Patent Publication Number: US-11661632-B2

Title: Compositions and methods for diagnosing lung cancers using gene expression profiles

Description:
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT 
     This invention was made with government support under Grant No. CA010815 awarded by the National Institutes of Health. The government has certain rights in the invention. 
    
    
     BACKGROUND OF THE INVENTION 
     Lung cancer is the most common worldwide cause of cancer mortality. In the United States, lung cancer is the second most prevalent cancer in both men and women and will account for more than 174,000 new cases per year and more than 162,000 cancer deaths. In fact, lung cancer accounts for more deaths each year than from breast, prostate and colorectal cancers combined. 
     The high mortality (80-85% in five years), which has shown little or no improvement in the past 30 years, emphasizes the fact that new and effective tools to facilitate early diagnosis prior to metastasis to regional nodes or beyond the lung are needed. 
     High risk populations include smokers, former smokers, and individuals with markers associated with genetic predispositions. Because surgical removal of early stage tumors remains the most effective treatment for lung cancer, there has been great interest in screening high-risk patients with low dose spiral CT (LDCT). This strategy identifies non-calcified pulmonary nodules in approximately 30-70% of high risk individuals but only a small proportion of detected nodules are ultimately diagnosed as lung cancers (0.4 to 2.7%). Currently, the only way to differentiate subjects with lung nodules of benign etiology from subjects with malignant nodules is an invasive biopsy, surgery, or prolonged observation with repeated scanning Even using the best clinical algorithms, 20-55% of patients selected to undergo surgical lung biopsy for indeterminate lung nodules, are found to have benign disease and those that do not undergo immediate biopsy or resection require sequential imaging studies. The use of serial CT in this group of patients runs the risk of delaying potential curable therapy, along with the costs of repeat scans, the not-insignificant radiation doses, and the anxiety of the patient. 
     Ideally, a diagnostic test would be easily accessible, inexpensive, demonstrate high sensitivity and specificity, and result in improved patient outcomes (medically and financially). Others have shown that classifiers which utilize epithelial cells have high accuracy. However, harvesting these cells requires an invasive bronchoscopy. See, Silvestri et al, N Engl J Med. 2015 Jul. 16; 373(3): 243-251, which is incorporated herein by reference. 
     Efforts are in progress to develop non-invasive diagnostics using sputum, blood or serum and analyzing for products of tumor cells, methylated tumor DNA, single nucleotide polymorphism (SNPs) expressed messenger RNA or proteins. This broad array of molecular tests with potential utility for early diagnosis of lung cancer has been discussed in the literature. Although each of these approaches has its own merits, none has yet passed the exploratory stage in the effort to detect patients with early stage lung cancer, even in high-risk groups, or patients which have a preliminary diagnosis based on radiological and other clinical factors. A simple blood test, a routine event associated with regular clinical office visits, would be an ideal diagnostic test. 
     SUMMARY OF THE INVENTION 
     In one aspect, a composition or kit for diagnosing or evaluating a lung cancer in a mammalian subject includes ten (10) or more polynucleotides or oligonucleotides, wherein each polynucleotide or oligonucleotide hybridizes to a different gene, gene fragment, gene transcript or expression product in a patient sample. Each gene, gene fragment, gene transcript or expression product is selected from the genes of Table I or Table II. In one embodiment, at least one polynucleotide or oligonucleotide is attached to a detectable label. In one embodiment, the composition or kit includes polynucleotides or oligonucleotides which detect the gene, gene fragment, gene transcript or expression product of each of the 559 genes in Table I. In another embodiment, the composition or kit includes polynucleotides or oligonucleotides which detect the gene, gene fragment, gene transcript or expression product of each of the 100 genes in Table II. 
     In another aspect, a composition or kit for diagnosing or evaluating a lung cancer in a mammalian subject includes ten (10) or more ligands, wherein each ligand hybridizes to a different gene expression product in a patient sample. Each gene expression product is selected from the genes of Table I or Table II. In one embodiment, at least one ligand is attached to a detectable label. In one embodiment, the composition or kit includes ligands which detect the expression products of each of the 559 genes in Table I. In another embodiment, the composition or kit includes ligands which detect the expression products of each of the 100 genes in Table II. 
     The compositions described herein enable detection of changes in expression in the genes in the subject&#39;s gene expression profile from that of a reference gene expression profile. The various reference gene expression profiles are described below. In one embodiment, the composition provides the ability to distinguish a cancerous tumor from a non-cancerous nodule. 
     In another aspect, a method for diagnosing or evaluating a lung cancer in a mammalian subject involves identifying changes in the expression of three or more genes in the sample of a subject, said genes selected from the genes of Table I or Table II, and comparing that subject&#39;s gene expression levels with the levels of the same genes in a reference or control, wherein changes in expression of said gene expression correlates with a diagnosis or evaluation of a lung cancer. In one embodiment, the changes in expression of said gene expression provides the ability to distinguish a cancerous tumor from a non-cancerous nodule. 
     In another aspect, a method for diagnosing or evaluating a lung cancer in a mammalian subject involves identifying a gene expression profile in the blood of a subject, the gene expression profile comprising 10 or more gene expression products of 10 or more informative genes as described herein. The 10 or more informative genes are selected from the genes of Table I or Table II. In one embodiment, the gene expression profile contains all 559 genes of Table I. In another embodiment, the gene expression profile contains all 100 genes of Table II. The subject&#39;s gene expression profile is compared with a reference gene expression profile from a variety of sources described below. Changes in expression of the informative genes correlate with a diagnosis or evaluation of a lung cancer. In one embodiment, the changes in expression of said gene expression provides the ability to distinguish a cancerous tumor from a non-cancerous nodule. 
     In another aspect, a method of detecting lung cancer in a patient is provided. The method includes obtaining a sample from the patient; and detecting a change in expression in at least 10 genes selected from Table I or Table II in the patient sample as compared to a control by contacting the sample with a composition comprising oligonucleotides, polynucleotides or ligands specific for each different gene transcript or expression product of the at least 10 gene of Table I or Table II and detecting binding between the oligonucleotide, polynucleotide or ligand and the gene product or expression product. 
     In yet another aspect, a method of diagnosing lung cancer in a subject is provided. The method includes obtaining a blood sample from a subject; detecting a change in expression in at least 10 genes selected from Table I or Table II in the patient sample as compared to a control by contacting the sample with a composition comprising oligonucleotides, polynucleotides or ligands specific for each different gene transcript or expression product of the at least 10 gene of Table I or Table II and detecting binding between the oligonucleotide, polynucleotide or ligand and the gene product or expression product; and diagnosing the subject with cancer when changes in expression of the subject&#39;s genes from those of the reference are detected. 
     In another aspect, a method of diagnosing and treating lung cancer in a subject having a neoplastic growth is provided. The method includes obtaining a blood sample from a subject; detecting a change in expression in at least 10 genes selected from Table I or Table II in the patient sample as compared to a control by contacting the sample with a composition comprising oligonucleotides, polynucleotides or ligands specific for each different gene transcript or expression product of the at least 10 gene of Table I or Table II and detecting binding between the oligonucleotide, polynucleotide or ligand and the gene product or expression product; diagnosing the subject with cancer when changes in expression of the subject&#39;s genes from those of the reference are detected; and removing the neoplastic growth. Other appropriate treatments may also be provided. 
     Other aspects and advantages of these compositions and methods are described further in the following detailed description of the preferred embodiments thereof. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG.  1    is a table showing patient characteristics for the samples used in Example 1. 
         FIGS.  2 A and  2 B  are graphs showing the cross validated support vector machine classifier (CV SVM) of all 610 samples ( FIG.  2 A , Accuracy=0.75, ROC Area=0.81. According to the curve, when the sensitivity is 0.91, the specificity is 0.46; when the sensitivity is 0.72, the specificity is 0.77) and a balanced set of 556 samples ( FIG.  2 B , Accuracy=0.76, ROC Area=0.81, According to the curve, when the sensitivity is 0.90, the specificity is 0.48; when the sensitivity is 0.76, the specificity is 0.77), using the 559 Classifier. The full and balanced sets show similar performance. 
         FIG.  3    is a bar graph showing sensitivity of the classifier by nodule size groups (x-axis). Data shows that larger nodules are more likely to be misclassified (p=1.54*10 −4 ). 
         FIGS.  4 A to  4 C  show the classification of samples groups (cancer,  FIG.  4 B , n=204; and nodule,  FIG.  4 C , n=331) stratified by lesion size. Over cancers &gt;5 mm and higher, r=0.95. For nodules of all sizes, r=0.97. The chart ( FIG.  4 A ) shows the sensitivity and specificity of the classification of cancers and nodules based on lesion size. These numbers are shown in bar graph form below. 
         FIGS.  5 A and  5 B  are graphs showing the cross validated support vector machine classifier (CV SVM) of all cancer samples (n=278) vs. small nodules (&lt;10 mm) (n=244) ( FIG.  5 A , Accuracy=0.79, ROC Area=0.85. According to the curve, when the sensitivity is 0.90, the specificity is 0.54; when the sensitivity is 0.77, the specificity is 0.82) and 10-fold CV SVM using all cancer samples (n=278) vs. large nodules (≥10 mm) (n=88) ( FIG.  5 B , Accuracy=0.76, ROC Area=0.71. According to the curve, when the sensitivity is 0.90, the specificity is 0.24; when the sensitivity is 0.87, the specificity is 0.42). 
         FIG.  6    is a graph showing the cross validated support vector machine classifier (CV SVM) of 25% of the data set used for the 559 Classifier, used as a testing set for the 100 Classifier. ROC Area=0.82. According to the curve, when the sensitivity is 0.90, the specificity is 0.62; when the sensitivity is 0.79, the specificity is 0.68; and when the sensitivity is 0.71, the specificity is 0.75. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The methods and compositions described herein apply gene expression technology to blood screening for the detection and diagnosis of lung cancer. The compositions and methods described herein provide the ability to distinguish a cancerous tumor from a non-cancerous nodule, by determining a characteristic RNA expression profile of the genes of the blood of a mammalian, preferably human, subject. The profile is compared with the profile of one or more subjects of the same class (e.g., patients having lung cancer or a non-cancerous nodule) or a control to provide a useful diagnosis. 
     These methods of lung cancer screening employ compositions suitable for conducting a simple and cost-effective and non-invasive blood test using gene expression profiling that could alert the patient and physician to obtain further studies, such as a chest radiograph or CT scan, in much the same way that the prostate specific antigen is used to help diagnose and follow the progress of prostate cancer. The application of these profiles provides overlapping and confirmatory diagnoses of the type of lung disease, beginning with the initial test for malignant vs. non-malignant disease. 
     “Patient” or “subject” as used herein means a mammalian animal, including a human, a veterinary or farm animal, a domestic animal or pet, and animals normally used for clinical research. In one embodiment, the subject of these methods and compositions is a human. 
     “Control” or “Control subject” as used herein refers to the source of the reference gene expression profiles as well as the particular panel of control subjects described herein. In one embodiment, the control or reference level is from a single subject. In another embodiment, the control or reference level is from a population of individuals sharing a specific characteristic. In yet another embodiment, the control or reference level is an assigned value which correlates with the level of a specific control individual or population, although not necessarily measured at the time of assaying the test subject&#39;s sample. In one embodiment, the control subject or reference is from a patient (or population) having a non-cancerous nodule. In another embodiment, the control subject or reference is from a patient (or population) having a cancerous tumor. In other embodiments, the control subject can be a subject or population with lung cancer, such as a subject who is a current or former smoker with malignant disease, a subject with a solid lung tumor prior to surgery for removal of same; a subject with a solid lung tumor following surgical removal of said tumor; a subject with a solid lung tumor prior to therapy for same; and a subject with a solid lung tumor during or following therapy for same. In other embodiments, the controls for purposes of the compositions and methods described herein include any of the following classes of reference human subject with no lung cancer. Such non-healthy controls (NHC) include the classes of smoker with non-malignant disease, a former smoker with non-malignant disease (including patients with lung nodules), a non-smoker who has chronic obstructive pulmonary disease (COPD), and a former smoker with COPD. In still other embodiments, the control subject is a healthy non-smoker with no disease or a healthy smoker with no disease. 
     “Sample” as used herein means any biological fluid or tissue that contains immune cells and/or cancer cells. The most suitable sample for use in this invention includes whole blood. Other useful biological samples include, without limitation, peripheral blood mononuclear cells, plasma, saliva, urine, synovial fluid, bone marrow, cerebrospinal fluid, vaginal mucus, cervical mucus, nasal secretions, sputum, semen, amniotic fluid, bronchoscopy sample, bronchoalveolar lavage fluid, and other cellular exudates from a patient having cancer. Such samples may further be diluted with saline, buffer or a physiologically acceptable diluent. Alternatively, such samples are concentrated by conventional means. 
     As used herein, the term “cancer” refers to or describes the physiological condition in mammals that is typically characterized by unregulated cell growth. More specifically, as used herein, the term “cancer” means any lung cancer. In one embodiment, the lung cancer is non-small cell lung cancer (NSCLC). In a more specific embodiment, the lung cancer is lung adenocarcinoma (AC or LAC). In another more specific embodiment, the lung cancer is lung squamous cell carcinoma (SCC or LSCC). In another embodiment, the lung cancer is a stage I or stage II NSCLC. In still another embodiment, the lung cancer is a mixture of early and late stages and types of NSCLC. 
     The term “tumor,” as used herein, refers to all neoplastic cell growth and proliferation, whether malignant or benign, and all pre-cancerous and cancerous cells and tissues. The term “nodule” refers to an abnormal buildup of tissue which is benign. The term “cancerous tumor” refers to a malignant tumor. 
     By “diagnosis” or “evaluation” it is meant a diagnosis of a lung cancer, a diagnosis of a stage of lung cancer, a diagnosis of a type or classification of a lung cancer, a diagnosis or detection of a recurrence of a lung cancer, a diagnosis or detection of a regression of a lung cancer, a prognosis of a lung cancer, or an evaluation of the response of a lung cancer to a surgical or non-surgical therapy. In one embodiment, “diagnosis” or “evaluation” refers to distinguishing between a cancerous tumor and a benign pulmonary nodule. 
     As used herein, “sensitivity” (also called the true positive rate), measures the proportion of positives that are correctly identified as such (e.g., the percentage of sick people who are correctly identified as having the condition). 
     As used herein, “specificity” (also called the true negative rate) measures the proportion of negatives that are correctly identified as such (e.g., the percentage of healthy people who are correctly identified as not having the condition). 
     By “change in expression” is meant an upregulation of one or more selected genes in comparison to the reference or control; a downregulation of one or more selected genes in comparison to the reference or control; or a combination of certain upregulated genes and down regulated genes. 
     By “therapeutic reagent” or “regimen” is meant any type of treatment employed in the treatment of cancers with or without solid tumors, including, without limitation, chemotherapeutic pharmaceuticals, biological response modifiers, radiation, diet, vitamin therapy, hormone therapies, gene therapy, surgical resection, etc. 
     By “informative genes” as used herein is meant those genes the expression of which changes (either in an up-regulated or down-regulated manner) characteristically in the presence of lung cancer. A statistically significant number of such informative genes thus form suitable gene expression profiles for use in the methods and compositions. Such genes are shown in Table I and Table II below. Such genes make up the “expression profile”. 
     The term “statistically significant number of genes” in the context of this invention differs depending on the degree of change in gene expression observed. The degree of change in gene expression varies with the type of cancer and with the size or spread of the cancer or solid tumor. The degree of change also varies with the immune response of the individual and is subject to variation with each individual. For example, in one embodiment of this invention, a large change, e.g., 2-3 fold increase or decrease in a small number of genes, e.g., in about 10 to 20 genes, is statistically significant. In another embodiment, a smaller relative change in about 15 more genes is statistically significant. 
     Thus, the methods and compositions described herein contemplate examination of the expression profile of a “statistically significant number of genes” ranging from 5 to about 559 genes in a single profile. In one embodiment, the genes are selected from Table I. In another embodiment, the genes are selected from Table II. In one embodiment, the gene profile is formed by a statistically significant number of 5 or more genes. In one embodiment, the gene profile is formed by a statistically significant number of 10 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 15 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 20 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 25 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 30 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 35 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 40 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 45 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 50 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 60 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 65 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 70 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 75 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 80 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 85 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 90 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 95 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 100 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 200 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 300 or more genes. In another embodiment, the gene profile is formed by a statistically significant number of 350 or more genes. In still another embodiment, the gene profile is formed by 400 or more genes. In still another embodiment, the gene profile is formed by 539 genes. In still another embodiment, the gene profile is formed by 559 genes. In still other embodiments, the gene profiles examined as part of these methods contain, as statistically significant numbers of genes, from 10 to 559 genes, and any numbers therebetween. In another embodiment, the gene profile is formed by 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 547, 548, 549, 550, 551, 552, 553, 554, 555, 556, 557, 558, or all 559 genes of Table I. In another embodiment, the gene profile is formed by 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or all 100 genes of Table II. 
     Table I and Table II below refer to a collection of known genes useful in discriminating between a subject having a lung cancer, e.g., NSCLC, and subjects having benign (non-malignant) lung nodules. The sequences of the genes identified in Table I and Table II are publicly available. One skilled in the art may readily reproduce the compositions and methods described herein by use of the sequences of the genes, all of which are publicly available from conventional sources, such as GenBank. The GenBank accession number for each gene is provided. 
     The term “microarray” refers to an ordered arrangement of hybridizable array elements, preferably polynucleotide or oligonucleotide probes, on a substrate. 
     The term “polynucleotide,” when used in singular or plural form, generally refers to any polyribonucleotide or polydeoxribonucleotide, which may be unmodified RNA or DNA or modified RNA or DNA. Thus, for instance, polynucleotides as defined herein include, without limitation, single- and double-stranded DNA, DNA including single- and double-stranded regions, single- and double-stranded RNA, and RNA including single- and double-stranded regions, hybrid molecules comprising DNA and RNA that may be single-stranded or, more typically, double-stranded or include single- and double-stranded regions. In addition, the term “polynucleotide” as used herein refers to triple-stranded regions comprising RNA or DNA or both RNA and DNA. The strands in such regions may be from the same molecule or from different molecules. The regions may include all of one or more of the molecules, but more typically involve only a region of some of the molecules. One of the molecules of a triple-helical region often is an oligonucleotide. The term “polynucleotide” specifically includes cDNAs. The term includes DNAs (including cDNAs) and RNAs that contain one or more modified bases. Thus, DNAs or RNAs with backbones modified for stability or for other reasons are “polynucleotides” as that term is intended herein. Moreover, DNAs or RNAs comprising unusual bases, such as inosine, or modified bases, such as tritiated bases, are included within the term “polynucleotides” as defined herein. In general, the term “polynucleotide” embraces all chemically, enzymatically and/or metabolically modified forms of unmodified polynucleotides, as well as the chemical forms of DNA and RNA characteristic of viruses and cells, including simple and complex cells. 
     The term “oligonucleotide” refers to a relatively short polynucleotide, including, without limitation, single-stranded deoxyribonucleotides, single- or double-stranded ribonucleotides, RNA:DNA hybrids and double-stranded DNAs. Oligonucleotides, such as single-stranded DNA probe oligonucleotides, are often synthesized by chemical methods, for example using automated oligonucleotide synthesizers that are commercially available. However, oligonucleotides can be made by a variety of other methods, including in vitro recombinant DNA-mediated techniques and by expression of DNAs in cells and organisms. 
     The terms “differentially expressed gene”, “differential gene expression” and their synonyms, which are used interchangeably, refer to a gene whose expression is activated to a higher or lower level in a subject suffering from a disease, specifically cancer, such as lung cancer, relative to its expression in a control subject, such as a subject having a benign nodule. The terms also include genes whose expression is activated to a higher or lower level at different stages of the same disease. It is also understood that a differentially expressed gene may be either activated or inhibited at the nucleic acid level or protein level, or may be subject to alternative splicing to result in a different polypeptide product. Such differences may be evidenced by a change in mRNA levels, surface expression, secretion or other partitioning of a polypeptide, for example. Differential gene expression may include a comparison of expression between two or more genes or their gene products, or a comparison of the ratios of the expression between two or more genes or their gene products, or even a comparison of two differently processed products of the same gene, which differ between normal subjects, non-health controls and subjects suffering from a disease, specifically cancer, or between various stages of the same disease. Differential expression includes both quantitative, as well as qualitative, differences in the temporal or cellular expression pattern in a gene or its expression products among, for example, normal and diseased cells, or among cells which have undergone different disease events or disease stages. For the purpose of this invention, “differential gene expression” is considered to be present when there is a statistically significant (p&lt;0.05) difference in gene expression between the subject and control samples. 
     The term “over-expression” with regard to an RNA transcript is used to refer to the level of the transcript determined by normalization to the level of reference mRNAs, which might be all measured transcripts in the specimen or a particular reference set of mRNAs. 
     The phrase “gene amplification” refers to a process by which multiple copies of a gene or gene fragment are formed in a particular cell or cell line. The duplicated region (a stretch of amplified DNA) is often referred to as “amplicon.” Usually, the amount of the messenger RNA (mRNA) produced, i.e., the level of gene expression, also increases in the proportion of the number of copies made of the particular gene expressed. 
     In the context of the compositions and methods described herein, reference to “10 or more”, “at least 10” etc. of the genes listed in Table I or Table II means any one or any and all combinations of the genes listed. For example, suitable gene expression profiles include profiles containing any number between at least 5 through 559 genes from Table I. In another example, suitable gene expression profiles include profiles containing any number between at least 5 through 100 genes from Table II. In one embodiment, gene profiles formed by genes selected from a table are used in rank order, e.g., genes ranked in the top of the list demonstrated more significant discriminatory results in the tests, and thus may be more significant in a profile than lower ranked genes. However, in other embodiments the genes forming a useful gene profile do not have to be in rank order and may be any gene from the table. As used herein, the term “100 Classifier” or “100 Biomarker Classifier” refers to the 100 genes of Table II. As used herein, the term “559 Classifier” or “559 Biomarker Classifier” refers to the 559 genes of Table I. However, subsets of the genes of Table I or Table II, as described herein, are also useful, and, in another embodiment, the terms may refer to those subsets as well. 
     As used herein, “labels” or “reporter molecules” are chemical or biochemical moieties useful for labeling a nucleic acid (including a single nucleotide), polynucleotide, oligonucleotide, or protein ligand, e.g., amino acid or antibody. “Labels” and “reporter molecules” include fluorescent agents, chemiluminescent agents, chromogenic agents, quenching agents, radionucleotides, enzymes, substrates, cofactors, inhibitors, magnetic particles, and other moieties known in the art. “Labels” or “reporter molecules” are capable of generating a measurable signal and may be covalently or noncovalently joined or bound to an oligonucleotide or nucleotide (e.g., a non-natural nucleotide) or ligand. 
     Unless defined otherwise in this specification, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs and by reference to published texts, which provide one skilled in the art with a general guide to many of the terms used in the present application. 
     I. GENE EXPRESSION PROFILES 
     The inventors have shown that the gene expression profiles of the whole blood of lung cancer patients differ significantly from those seen in patients having non-cancerous lung nodules. For example, changes in the gene expression products of the genes of Table I and/or Table II can be observed and detected by the methods of this invention in the normal circulating blood of patients with early stage solid lung tumors. 
     The gene expression profiles described herein provide new diagnostic markers for the early detection of lung cancer and could prevent patients from undergoing unnecessary procedures relating to surgery or biopsy for a benign nodule. Since the risks are very low, the benefit to risk ratio is very high. In one embodiment, the methods and compositions described herein may be used in conjunction with clinical risk factors to help physicians make more accurate decisions about how to manage patients with lung nodules. Another advantage of this invention is that diagnosis may occur early since diagnosis is not dependent upon detecting circulating tumor cells which are present in only vanishing small numbers in early stage lung cancers. 
     In one aspect, a composition is provided for classifying a nodule as cancerous or benign in a mammalian subject. In one embodiment, the composition includes at least 10 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In another embodiment, the composition includes at least 100 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In one embodiment, the polynucleotide or oligonucleotide or ligand hybridizes to an mRNA. 
     
       
         
           
               
               
               
               
             
               
                 TABLE I 
               
               
                   
               
               
                 Rank 
                 Sequence ID# 
                 Gene 
                 Class Name 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 1 
                 PLEKHG4 
                 NM_015432.3 
                 Endogenous 
               
               
                 2 
                 SLC25A20 
                 NM_000387.5 
                 Endogenous 
               
               
                 3 
                 LETM2 
                 NM_144652.3 
                 Endogenous 
               
               
                 4 
                 GLIS3 
                 NM_001042413.1 
                 Endogenous 
               
               
                 5 
                 LOC100132797 
                 XR_036994.1 
                 Endogenous 
               
               
                 6 
                 ARHGEF5 
                 NM_005435.3 
                 Endogenous 
               
               
                 7 
                 TCF7L2 
                 NM_030756.4 
                 Endogenous 
               
               
                 8 
                 SFRS2IP 
                 NM_004719.2 
                 Endogenous 
               
               
                 9 
                 CFD 
                 NM_001928.2 
                 Endogenous 
               
               
                 10 
                 AZI2 
                 NM_022461.4 
                 Endogenous 
               
               
                 11 
                 STOM 
                 NM_004099.5 
                 Endogenous 
               
               
                 12 
                 CD1A 
                 NM_001763.2 
                 Endogenous 
               
               
                 13 
                 PANK2 
                 NM_153640.2 
                 Endogenous 
               
               
                 14 
                 CNIH4 
                 NM_014184.3 
                 Endogenous 
               
               
                 15 
                 EVI2A 
                 NM_014210.3 
                 Endogenous 
               
               
                 16 
                 BATF 
                 NM_006399.3 
                 Endogenous 
               
               
                 17 
                 TCP1 
                 NM_030752.2 
                 Endogenous 
               
               
                 18 
                 BX108566 
                 BX108566.1 
                 Endogenous 
               
               
                 19 
                 ANXA1 
                 NM_000700.2 
                 Endogenous 
               
               
                 20 
                 PSMA3 
                 NM_152132.2 
                 Endogenous 
               
               
                 21 
                 IRF4 
                 NM_002460.1 
                 Endogenous 
               
               
                 22 
                 STAG3 
                 NM_012447.3 
                 Endogenous 
               
               
                 23 
                 NDUFS4 
                 NM_002495.2 
                 Endogenous 
               
               
                 24 
                 HAT1 
                 NM_003642.3 
                 Endogenous 
               
               
                 25 
                 ANXA1 b 
                 NM_000700.1 
                 Endogenous 
               
               
                 26 
                 LOC148137 
                 NM_144692.1 
                 Endogenous 
               
               
                 27 
                 LDHA 
                 NM_001165416.1 
                 Endogenous 
               
               
                 28 
                 PSME3 
                 NM_005789.3 
                 Endogenous 
               
               
                 29 
                 REPS1 
                 NM_001128617.2 
                 Endogenous 
               
               
                 30 
                 CDH5 
                 NM_001795.3 
                 Endogenous 
               
               
                 31 
                 NAT5 
                 NM_181528.3 
                 Endogenous 
               
               
                 32 
                 PLAC8 
                 NM_001130715.1 
                 Endogenous 
               
               
                 33 
                 GSTO1 
                 NM_004832.2 
                 Endogenous 
               
               
                 34 
                 DGUOK 
                 NM_080916.2 
                 Endogenous 
               
               
                 35 
                 OLR1 
                 NM_002543.3 
                 Endogenous 
               
               
                 36 
                 MYST4 
                 NM_012330.3 
                 Endogenous 
               
               
                 37 
                 TIMM8B 
                 ENST00000504148.1 
                 Endogenous 
               
               
                 38 
                 LY96 
                 NM_015364.4 
                 Endogenous 
               
               
                 39 
                 CCDC72 
                 NM_015933.4 
                 Endogenous 
               
               
                 40 
                 ATP5I 
                 NM_007100.2 
                 Endogenous 
               
               
                 41 
                 WDR91 
                 NM_014149.3 
                 Endogenous 
               
               
                 42 
                 MAGEA3 
                 NM_005362.3 
                 Endogenous 
               
               
                 43 
                 AK093878 
                 AK093878.1 
                 Endogenous 
               
               
                 44 
                 EYA3 
                 NM_001990.3 
                 Endogenous 
               
               
                 45 
                 ACAA2 
                 NM_006111.2 
                 Endogenous 
               
               
                 46 
                 ETFDH 
                 NM_004453.3 
                 Endogenous 
               
               
                 47 
                 CCT6A 
                 NM_001762.3 
                 Endogenous 
               
               
                 48 
                 HSCB 
                 NM_172002.3 
                 Endogenous 
               
               
                 49 
                 EMR4 
                 NM_001080498.2 
                 Endogenous 
               
               
                 50 
                 USP5 
                 NM_003481.2 
                 Endogenous 
               
               
                 51 
                 SIK1 
                 NM_173354.3 
                 Endogenous 
               
               
                 52 
                 SYNJ1 
                 NM_003895.3 
                 Endogenous 
               
               
                 53 
                 KLRB1 
                 NM_002258.2 
                 Endogenous 
               
               
                 54 
                 CLK2 
                 XM_941392.1 
                 Endogenous 
               
               
                 55 
                 SNORA56 
                 NR_002984.1 
                 Endogenous 
               
               
                 56 
                 TP53BP1 
                 NM_005657.2 
                 Endogenous 
               
               
                 57 
                 RBX1 
                 NM_014248.3 
                 Endogenous 
               
               
                 58 
                 CNPY2 
                 NM_014255.5 
                 Endogenous 
               
               
                 59 
                 RELA 
                 NM_021975.2 
                 Endogenous 
               
               
                 60 
                 LOC732371 
                 XM_001133019.1 
                 Endogenous 
               
               
                 61 
                 TMEM218 
                 NM_001080546.2 
                 Endogenous 
               
               
                 62 
                 LOC91431 
                 NM_001099776.1 
                 Endogenous 
               
               
                 63 
                 GZMB 
                 NM_004131.3 
                 Endogenous 
               
               
                 64 
                 CAMP 
                 NM_004345.4 
                 Endogenous 
               
               
                 65 
                 RBM16 
                 NM_014892.4 
                 Endogenous 
               
               
                 66 
                 MID1IP1 
                 NM_021242.5 
                 Endogenous 
               
               
                 67 
                 LOC399942 
                 XM_934471.1 
                 Endogenous 
               
               
                 68 
                 COMMD6 
                 NM_203497.3 
                 Endogenous 
               
               
                 69 
                 PPP6C 
                 NM_002721.4 
                 Endogenous 
               
               
                 70 
                 BCOR 
                 NM_017745.5 
                 Endogenous 
               
               
                 71 
                 PDCD10 
                 NM_145859.1 
                 Endogenous 
               
               
                 72 
                 HLA-DMB 
                 NM_002118.3 
                 Endogenous 
               
               
                 73 
                 DNAJB1 
                 NM_006145.2 
                 Endogenous 
               
               
                 74 
                 KYNU 
                 NM_001032998.1 
                 Endogenous 
               
               
                 75 
                 TM2D2 
                 NM_078473.2 
                 Endogenous 
               
               
                 76 
                 FAM179A 
                 NM_199280.2 
                 Endogenous 
               
               
                 77 
                 FAM43A 
                 NM_153690.4 
                 Endogenous 
               
               
                 78 
                 QTRTD1 
                 NM_024638.3 
                 Endogenous 
               
               
                 79 
                 MARCKSL1 
                 NM_023009.5 
                 Endogenous 
               
               
                 80 
                 FAM193A 
                 NM_003704.3 
                 Endogenous 
               
               
                 81 
                 AK026725 
                 AK026725.1 
                 Endogenous 
               
               
                 82 
                 SERPINB10 
                 NM_005024.1 
                 Endogenous 
               
               
                 83 
                 OSBP 
                 ILMN_1706376.1 
                 Endogenous 
               
               
                 84 
                 ST6GAL1 
                 NM_003032.2 
                 Endogenous 
               
               
                 85 
                 NDUFAF2 
                 NM_174889.4 
                 Endogenous 
               
               
                 86 
                 UBE2I 
                 NM_194259.2 
                 Endogenous 
               
               
                 87 
                 CTAG1B 
                 NM_001327.2 
                 Endogenous 
               
               
                 88 
                 TRAF6 
                 NM_145803.1 
                 Endogenous 
               
               
                 89 
                 REPIN1 
                 NM_014374.3 
                 Endogenous 
               
               
                 90 
                 LAMA5 
                 NM_005560.4 
                 Endogenous 
               
               
                 91 
                 TBC1D12 
                 NM_015188.1 
                 Endogenous 
               
               
                 92 
                 TGIF1 b 
                 NM_173208.1 
                 Endogenous 
               
               
                 93 
                 LOC728533 
                 XR_015610.3 
                 Endogenous 
               
               
                 94 
                 CLN8 
                 NM_018941.3 
                 Endogenous 
               
               
                 95 
                 COX7B 
                 NM_001866.2 
                 Endogenous 
               
               
                 96 
                 DYNC2LI1 
                 NM_016008.3 
                 Endogenous 
               
               
                 97 
                 ANP32B 
                 NM_006401.2 
                 Endogenous 
               
               
                 98 
                 PTGDR2 
                 NM_004778.1 
                 Endogenous 
               
               
                 99 
                 MRPS16 
                 NM_016065.3 
                 Endogenous 
               
               
                 100 
                 NIPBL 
                 NM_133433.3 
                 Endogenous 
               
               
                 101 
                 PPP2R5C 
                 NM_178588.1 
                 Endogenous 
               
               
                 102 
                 DPF2 
                 NM_006268.4 
                 Endogenous 
               
               
                 103 
                 RAB10 
                 NM_016131.4 
                 Endogenous 
               
               
                 104 
                 MYADM 
                 NM_001020820.1 
                 Endogenous 
               
               
                 105 
                 CCND3 
                 NM_001760.2 
                 Endogenous 
               
               
                 106 
                 CC2D1B 
                 NM_032449.2 
                 Endogenous 
               
               
                 107 
                 HLA-G 
                 NM_002127.4 
                 Endogenous 
               
               
                 108 
                 CKS2 
                 NM_001827.1 
                 Endogenous 
               
               
                 109 
                 HPSE 
                 NM_006665.5 
                 Endogenous 
               
               
                 110 
                 UBE2G1 
                 NM_003342.4 
                 Endogenous 
               
               
                 111 
                 MED16 
                 NM_005481.2 
                 Endogenous 
               
               
                 112 
                 LOC339674 
                 XM_934917.1 
                 Endogenous 
               
               
                 113 
                 RNF114 
                 NM_018683.3 
                 Endogenous 
               
               
                 114 
                 KIR2DS3 
                 NM_012313.1 
                 Endogenous 
               
               
                 115 
                 AMD1 
                 NM_001634.4 
                 Endogenous 
               
               
                 116 
                 S100A8 
                 NM_002964.4 
                 Endogenous 
               
               
                 117 
                 NFATC4 
                 NM_001136022.2 
                 Endogenous 
               
               
                 118 
                 RPL39L 
                 NM_052969.1 
                 Endogenous 
               
               
                 119 
                 LOC399753 
                 XM_930634.1 
                 Endogenous 
               
               
                 120 
                 FKBP1A 
                 NM_054014.3 
                 Endogenous 
               
               
                 121 
                 CHMP5 
                 NM_016410.5 
                 Endogenous 
               
               
                 122 
                 CABC1 
                 NM_020247.4 
                 Endogenous 
               
               
                 123 
                 HLA-B 
                 NM_005514.6 
                 Endogenous 
               
               
                 124 
                 TRIM39 
                 NM_021253.3 
                 Endogenous 
               
               
                 125 
                 LOC645914 
                 XM_928884.1 
                 Endogenous 
               
               
                 126 
                 CD79A 
                 NM_021601.3 
                 Endogenous 
               
               
                 127 
                 GLRX 
                 ILMN_1737308.1 
                 Endogenous 
               
               
                 128 
                 RPL26L1 
                 NM_016093.2 
                 Endogenous 
               
               
                 129 
                 USP21 
                 NM_012475.4 
                 Endogenous 
               
               
                 130 
                 CD70 
                 NM_001252.2 
                 Endogenous 
               
               
                 131 
                 SPINK5 
                 NM_006846.3 
                 Endogenous 
               
               
                 132 
                 HUWE1 
                 NM_031407.6 
                 Endogenous 
               
               
                 133 
                 STK38 
                 NM_007271.3 
                 Endogenous 
               
               
                 134 
                 SEMG1 
                 NM_003007.2 
                 Endogenous 
               
               
                 135 
                 NDUFA4 
                 NM_002489.3 
                 Endogenous 
               
               
                 136 
                 MYADM b 
                 NM_001020820.1 
                 Endogenous 
               
               
                 137 
                 SGK1 b 
                 NM_005627.3 
                 Endogenous 
               
               
                 138 
                 SLAMF8 
                 NM_020125.2 
                 Endogenous 
               
               
                 139 
                 LOC653773 
                 XM_938755.1 
                 Endogenous 
               
               
                 140 
                 RPS24 
                 NM_001026.4 
                 Endogenous 
               
               
                 141 
                 LOC338799 
                 NR_002809.2 
                 Endogenous 
               
               
                 142 
                 MAP3K7 
                 NM_145333.1 
                 Endogenous 
               
               
                 143 
                 KLRD1 
                 NM_002262.3 
                 Endogenous 
               
               
                 144 
                 LOC732111 
                 XM_001134275.1 
                 Endogenous 
               
               
                 145 
                 CD69 
                 NM_001781.2 
                 Endogenous 
               
               
                 146 
                 DDIT4 
                 NM_019058.2 
                 Endogenous 
               
               
                 147 
                 C1orf222 
                 NM_001003808.1 
                 Endogenous 
               
               
                 148 
                 PFAS 
                 NM_012393.2 
                 Endogenous 
               
               
                 149 
                 USP9Y 
                 NM_004654.3 
                 Endogenous 
               
               
                 150 
                 COLEC12 
                 NM_130386.2 
                 Endogenous 
               
               
                 151 
                 VPS37C 
                 NM_017966.4 
                 Endogenous 
               
               
                 152 
                 SAP130 
                 NM_024545.3 
                 Endogenous 
               
               
                 153 
                 CDC42EP2 
                 NM_006779.3 
                 Endogenous 
               
               
                 154 
                 LOC643319 
                 XM_927980.1 
                 Endogenous 
               
               
                 155 
                 ASF1B 
                 NM_018154.2 
                 Endogenous 
               
               
                 156 
                 AK094576 
                 AK094576.1 
                 Endogenous 
               
               
                 157 
                 BANP 
                 NM_079837.2 
                 Endogenous 
               
               
                 158 
                 TBK1 
                 NM_013254.2 
                 Endogenous 
               
               
                 159 
                 GNS 
                 NM_002076.3 
                 Endogenous 
               
               
                 160 
                 IL1R2 
                 NM_173343.1 
                 Endogenous 
               
               
                 161 
                 CLEC4C 
                 NM_203503.1 
                 Endogenous 
               
               
                 162 
                 TM9SF1 
                 NM_006405.6 
                 Endogenous 
               
               
                 163 
                 PTGDR 
                 NM_000953.2 
                 Endogenous 
               
               
                 164 
                 GOLGA3 
                 NM_005895.3 
                 Endogenous 
               
               
                 165 
                 CLEC4A 
                 NM_194448.2 
                 Endogenous 
               
               
                 166 
                 TSC1 
                 NM_000368.4 
                 Endogenous 
               
               
                 167 
                 SFMBT1 
                 NM_001005158.2 
                 Endogenous 
               
               
                 168 
                 GLT25D1 
                 NM_024656.2 
                 Endogenous 
               
               
                 169 
                 LOC100130229 
                 XM_001717158.1 
                 Endogenous 
               
               
                 170 
                 PHF8 
                 NM_015107.2 
                 Endogenous 
               
               
                 171 
                 PUM1 
                 NM_001020658.1 
                 Endogenous 
               
               
                 172 
                 SMARCC1 
                 NM_003074.3 
                 Endogenous 
               
               
                 173 
                 AK126342 
                 AK126342.1 
                 Endogenous 
               
               
                 174 
                 ACSL5 
                 NM_203379.1 
                 Endogenous 
               
               
                 175 
                 TGIF1 
                 NM_003244.2 
                 Endogenous 
               
               
                 176 
                 BF375676 
                 BF375676.1 
                 Endogenous 
               
               
                 177 
                 SPA17 
                 NM_017425.3 
                 Endogenous 
               
               
                 178 
                 FLNB 
                 NM_001457.3 
                 Endogenous 
               
               
                 179 
                 FAM105B 
                 NM_138348.4 
                 Endogenous 
               
               
                 180 
                 CPPED1 
                 NM_018340.2 
                 Endogenous 
               
               
                 181 
                 TRIM32 
                 NM_012210.3 
                 Endogenous 
               
               
                 182 
                 RNF34 
                 NM_025126.3 
                 Endogenous 
               
               
                 183 
                 SLC45A3 
                 NM_033102.2 
                 Endogenous 
               
               
                 184 
                 P2RY10 
                 NM_198333.1 
                 Endogenous 
               
               
                 185 
                 AKR1C3 
                 NM_003739.4 
                 Endogenous 
               
               
                 186 
                 NME1-NME2 
                 NM_001018136.2 
                 Endogenous 
               
               
                 187 
                 AMPD3 
                 NM_000480.2 
                 Endogenous 
               
               
                 188 
                 HSP90AB1 
                 NM_007355.3 
                 Endogenous 
               
               
                 189 
                 RBM4B 
                 NM_031492.3 
                 Endogenous 
               
               
                 190 
                 DMBT1 
                 NM_007329.2 
                 Endogenous 
               
               
                 191 
                 TMCO1 
                 NM_019026.3 
                 Endogenous 
               
               
                 192 
                 CASP2 
                 NM_032983.3 
                 Endogenous 
               
               
                 193 
                 C1orf103 
                 NM_018372.3 
                 Endogenous 
               
               
                 194 
                 ARHGAP17 
                 NM_018054.5 
                 Endogenous 
               
               
                 195 
                 IFNA17 
                 NM_021268.2 
                 Endogenous 
               
               
                 196 
                 CTSZ 
                 NM_001336.3 
                 Endogenous 
               
               
                 197 
                 DBI 
                 NM_001079862.1 
                 Endogenous 
               
               
                 198 
                 TXNRD1 b 
                 NM_182743.2 
                 Endogenous 
               
               
                 199 
                 KIAA0460 
                 NM_015203.4 
                 Endogenous 
               
               
                 200 
                 PDGFD 
                 NM_033135.3 
                 Endogenous 
               
               
                 201 
                 ATG5 
                 NM_004849.2 
                 Endogenous 
               
               
                 202 
                 ITFG2 
                 NM_018463.3 
                 Endogenous 
               
               
                 203 
                 HERC1 
                 NM_003922.3 
                 Endogenous 
               
               
                 204 
                 MEN1 
                 NM_130799.2 
                 Endogenous 
               
               
                 205 
                 IFI27L2 
                 NM_032036.2 
                 Endogenous 
               
               
                 206 
                 LOC729887 
                 XR_040891.2 
                 Endogenous 
               
               
                 207 
                 PI4K2A 
                 NM_018425.3 
                 Endogenous 
               
               
                 208 
                 RAG1 
                 NM_000448.2 
                 Endogenous 
               
               
                 209 
                 CREB5 
                 NM_182898.3 
                 Endogenous 
               
               
                 210 
                 SLC6A12 
                 NM_003044.4 
                 Endogenous 
               
               
                 211 
                 CDKN1A 
                 NM_000389.2 
                 Endogenous 
               
               
                 212 
                 AW173314 
                 AW173314.1 
                 Endogenous 
               
               
                 213 
                 SAP130 b 
                 NM_024545.3 
                 Endogenous 
               
               
                 214 
                 ABCA5 
                 NM_018672.4 
                 Endogenous 
               
               
                 215 
                 SLC25A37 
                 NM_016612.2 
                 Endogenous 
               
               
                 216 
                 MYLIP 
                 NM_013262.3 
                 Endogenous 
               
               
                 217 
                 GATA2 
                 NM_001145662.1 
                 Endogenous 
               
               
                 218 
                 ATP5L 
                 NM_006476.4 
                 Endogenous 
               
               
                 219 
                 RPS27L 
                 NM_015920.3 
                 Endogenous 
               
               
                 220 
                 DB338252 
                 DB338252.1 
                 Endogenous 
               
               
                 221 
                 FRAT2 
                 NM_012083.2 
                 Endogenous 
               
               
                 222 
                 CCL4 
                 NM_002984.2 
                 Endogenous 
               
               
                 223 
                 CD79B 
                 NM_000626.2 
                 Endogenous 
               
               
                 224 
                 MBD1 
                 NM_015844.2 
                 Endogenous 
               
               
                 225 
                 TIAM1 
                 NM_003253.2 
                 Endogenous 
               
               
                 226 
                 HSD11B1 
                 NM_181755.1 
                 Endogenous 
               
               
                 227 
                 TPR 
                 NM_003292.2 
                 Endogenous 
               
               
                 228 
                 EID2B 
                 NM_152361.2 
                 Endogenous 
               
               
                 229 
                 PDSS1 
                 NM_014317.3 
                 Endogenous 
               
               
                 230 
                 C9orf164 
                 NM_182635.1 
                 Endogenous 
               
               
                 231 
                 ARHGEF18 
                 NM_015318.3 
                 Endogenous 
               
               
                 232 
                 TXNRD1 
                 NM_001093771.2 
                 Endogenous 
               
               
                 233 
                 HNRNPAB 
                 NM_004499.3 
                 Endogenous 
               
               
                 234 
                 TTN 
                 NM_133378.4 
                 Endogenous 
               
               
                 235 
                 EP300 
                 NM_001429.2 
                 Endogenous 
               
               
                 236 
                 CCDC97 
                 NM_052848.1 
                 Endogenous 
               
               
                 237 
                 HK3 
                 NM_002115.2 
                 Endogenous 
               
               
                 238 
                 CRKL 
                 NM_005207.3 
                 Endogenous 
               
               
                 239 
                 NCOA5 
                 NM_020967.2 
                 Endogenous 
               
               
                 240 
                 AK124143 
                 AK124143.1 
                 Endogenous 
               
               
                 241 
                 LBA1 
                 NM_014831.2 
                 Endogenous 
               
               
                 242 
                 SLC9A3R1 
                 NM_004252.3 
                 Endogenous 
               
               
                 243 
                 CRY2 
                 NM_021117.3 
                 Endogenous 
               
               
                 244 
                 ATG4B 
                 NM_178326.2 
                 Endogenous 
               
               
                 245 
                 CD97 
                 NM_078481.3 
                 Endogenous 
               
               
                 246 
                 TTC9 
                 NM_015351.1 
                 Endogenous 
               
               
                 247 
                 BMPR2 
                 NM_001204.6 
                 Endogenous 
               
               
                 248 
                 LPIN2 
                 NM_014646.2 
                 Endogenous 
               
               
                 249 
                 UBA1 
                 NM_003334.3 
                 Endogenous 
               
               
                 250 
                 SETD1B 
                 XM_037523.11 
                 Endogenous 
               
               
                 251 
                 PRPF8 
                 NM_006445.3 
                 Endogenous 
               
               
                 252 
                 RNASE2 
                 NM_002934.2 
                 Endogenous 
               
               
                 253 
                 KIAA0101 
                 NM_014736.4 
                 Endogenous 
               
               
                 254 
                 ARG1 
                 NM_000045.3 
                 Endogenous 
               
               
                 255 
                 UBTF 
                 NM_001076683.1 
                 Endogenous 
               
               
                 256 
                 MFSD1 
                 NM_022736.2 
                 Endogenous 
               
               
                 257 
                 IDO1 
                 NM_002164.3 
                 Endogenous 
               
               
                 258 
                 MS4A6A 
                 NM_022349.3 
                 Endogenous 
               
               
                 259 
                 C22orf30 
                 NM_173566.2 
                 Endogenous 
               
               
                 260 
                 HNRNPK 
                 NM_031263.2 
                 Endogenous 
               
               
                 261 
                 ARL8B 
                 NM_018184.2 
                 Endogenous 
               
               
                 262 
                 SETD2 
                 NM_014159.6 
                 Endogenous 
               
               
                 263 
                 NCAPG 
                 NM_022346.4 
                 Endogenous 
               
               
                 264 
                 EEF1B2 
                 NM_001037663.1 
                 Endogenous 
               
               
                 265 
                 TRIM39 b 
                 NM_172016.2 
                 Endogenous 
               
               
                 266 
                 EHD4 
                 NM_139265.3 
                 Endogenous 
               
               
                 267 
                 IRF1 
                 NM_002198.1 
                 Endogenous 
               
               
                 268 
                 LOC100129022 
                 XM_001716591.1 
                 Endogenous 
               
               
                 269 
                 TRAF3IP2 
                 NM_147686.3 
                 Endogenous 
               
               
                 270 
                 PSMA6 
                 NM_002791.2 
                 Endogenous 
               
               
                 271 
                 RHOG 
                 NM_001665.3 
                 Endogenous 
               
               
                 272 
                 CN312986 
                 CN312986.1 
                 Endogenous 
               
               
                 273 
                 PSMB8 
                 NM_004159.4 
                 Endogenous 
               
               
                 274 
                 ZNF239 
                 NM_001099283.1 
                 Endogenous 
               
               
                 275 
                 CLPTM1 
                 NM_001294.3 
                 Endogenous 
               
               
                 276 
                 NADK 
                 NM_023018.4 
                 Endogenous 
               
               
                 277 
                 C8orf76 
                 NM_032847.2 
                 Endogenous 
               
               
                 278 
                 LIF 
                 NM_002309.3 
                 Endogenous 
               
               
                 279 
                 EGR1 
                 NM_001964.2 
                 Endogenous 
               
               
                 280 
                 ARG1 b 
                 NM_000045.2 
                 Endogenous 
               
               
                 281 
                 MERTK 
                 NM_006343.2 
                 Endogenous 
               
               
                 282 
                 RHOU 
                 NM_021205.5 
                 Endogenous 
               
               
                 283 
                 PFDN5 b 
                 NM_145897.2 
                 Endogenous 
               
               
                 284 
                 MAGEA1 
                 NM_004988.4 
                 Endogenous 
               
               
                 285 
                 SEC24C 
                 NM_198597.2 
                 Endogenous 
               
               
                 286 
                 SLC11A1 
                 NM_000578.3 
                 Endogenous 
               
               
                 287 
                 TCF20 
                 NM_181492.2 
                 Endogenous 
               
               
                 288 
                 AHCYL1 
                 NM_001242676.1 
                 Endogenous 
               
               
                 289 
                 TPT1 
                 NM_003295.3 
                 Endogenous 
               
               
                 290 
                 KIR2DL5A 
                 XM_001126354.1 
                 Endogenous 
               
               
                 291 
                 IRAK2 
                 NM_001570.3 
                 Endogenous 
               
               
                 292 
                 C17orf51 
                 XM_944416.1 
                 Endogenous 
               
               
                 293 
                 C14orf156 
                 NM_031210.5 
                 Endogenous 
               
               
                 294 
                 ATP2C1 
                 NM_014382.3 
                 Endogenous 
               
               
                 295 
                 SOCS1 
                 NM_003745.1 
                 Endogenous 
               
               
                 296 
                 JAK1 
                 NM_002227.1 
                 Endogenous 
               
               
                 297 
                 RSL24D1 
                 NM_016304.2 
                 Endogenous 
               
               
                 298 
                 AP2S1 
                 NM_021575.3 
                 Endogenous 
               
               
                 299 
                 PHRF1 
                 NM_020901.3 
                 Endogenous 
               
               
                 300 
                 GPI 
                 NM_000175.2 
                 Endogenous 
               
               
                 301 
                 NCR1 
                 NM_004829.5 
                 Endogenous 
               
               
                 302 
                 AKAP4 
                 NM_139289.1 
                 Endogenous 
               
               
                 303 
                 CD160 
                 NM_007053.3 
                 Endogenous 
               
               
                 304 
                 DDX23 
                 NM_004818.2 
                 Endogenous 
               
               
                 305 
                 GNL3 
                 NM_014366.4 
                 Endogenous 
               
               
                 306 
                 NFKB2 
                 NM_002502.2 
                 Endogenous 
               
               
                 307 
                 CSK 
                 NM_004383.2 
                 Endogenous 
               
               
                 308 
                 PELP1 
                 NM_014389.2 
                 Endogenous 
               
               
                 309 
                 KLRF1 b 
                 NM_016523.2 
                 Endogenous 
               
               
                 310 
                 CS 
                 NM_004077.2 
                 Endogenous 
               
               
                 311 
                 PHCA 
                 NM_018367.6 
                 Endogenous 
               
               
                 312 
                 LOC644315 
                 XR_017529.2 
                 Endogenous 
               
               
                 313 
                 NUDT18 
                 NM_024815.3 
                 Endogenous 
               
               
                 314 
                 XCL2 
                 NM_003175.3 
                 Endogenous 
               
               
                 315 
                 KLRC1 
                 NM_002259.3 
                 Endogenous 
               
               
                 316 
                 ARHGAP18 
                 NM_033515.2 
                 Endogenous 
               
               
                 317 
                 CTDSP2 
                 NM_005730.3 
                 Endogenous 
               
               
                 318 
                 P2RY5 
                 NM_005767.5 
                 Endogenous 
               
               
                 319 
                 CREB1 
                 NM_004379.3 
                 Endogenous 
               
               
                 320 
                 RHOB 
                 NM_004040.3 
                 Endogenous 
               
               
                 321 
                 DCAF7 
                 NM_005828.4 
                 Endogenous 
               
               
                 322 
                 NUP153 
                 NM_005124.3 
                 Endogenous 
               
               
                 323 
                 AFTPH 
                 NM_017657.4 
                 Endogenous 
               
               
                 324 
                 EWSR1 
                 NM_005243.3 
                 Endogenous 
               
               
                 325 
                 LYN 
                 NM_002350.1 
                 Endogenous 
               
               
                 326 
                 CYBB 
                 NM_000397.3 
                 Endogenous 
               
               
                 327 
                 TMEM70 
                 NM_017866.5 
                 Endogenous 
               
               
                 328 
                 PPP1R3E 
                 XM_927029.1 
                 Endogenous 
               
               
                 329 
                 PSMB1 
                 NM_002793.3 
                 Endogenous 
               
               
                 330 
                 RERE b 
                 NM_012102.3 
                 Endogenous 
               
               
                 331 
                 RXRA 
                 NM_002957.5 
                 Endogenous 
               
               
                 332 
                 GZMA 
                 NM_006144.3 
                 Endogenous 
               
               
                 333 
                 ERLIN1 
                 NM_006459.3 
                 Endogenous 
               
               
                 334 
                 KRTAP10-3 
                 NM_198696.2 
                 Endogenous 
               
               
                 335 
                 SAMSN1 
                 NM_022136.3 
                 Endogenous 
               
               
                 336 
                 LRRC47 
                 NM_020710.2 
                 Endogenous 
               
               
                 337 
                 MARCKS 
                 NM_002356.6 
                 Endogenous 
               
               
                 338 
                 HOPX 
                 NM_139211.4 
                 Endogenous 
               
               
                 339 
                 KLRF1 
                 NM_016523.1 
                 Endogenous 
               
               
                 340 
                 NFAT5 
                 NM_138713.3 
                 Endogenous 
               
               
                 341 
                 SLC15A2 
                 NM_021082.3 
                 Endogenous 
               
               
                 342 
                 STK16 
                 NM_003691.2 
                 Endogenous 
               
               
                 343 
                 KIR_Activating_Subgroup_2 
                 NM_014512.1 
                 Endogenous 
               
               
                 344 
                 TBCE 
                 NM_001079515.2 
                 Endogenous 
               
               
                 345 
                 BAG3 
                 NM_004281.3 
                 Endogenous 
               
               
                 346 
                 SFRS4 
                 NM_005626.4 
                 Endogenous 
               
               
                 347 
                 AW270402 
                 AW270402.1 
                 Endogenous 
               
               
                 348 
                 CCL3L1 
                 NM_021006.4 
                 Endogenous 
               
               
                 349 
                 HERC3 
                 NM_014606.2 
                 Endogenous 
               
               
                 350 
                 RPL34 
                 NM_000995.3 
                 Endogenous 
               
               
                 351 
                 ALAS1 
                 NM_000688.4 
                 Endogenous 
               
               
                 352 
                 CCR9 
                 NM_031200.1 
                 Endogenous 
               
               
                 353 
                 CORO1C 
                 ILMN_1745954.1 
                 Endogenous 
               
               
                 354 
                 FAIM3 
                 NM_005449.4 
                 Endogenous 
               
               
                 355 
                 SFPQ 
                 NM_005066.2 
                 Endogenous 
               
               
                 356 
                 HOOK3 
                 NM_032410.3 
                 Endogenous 
               
               
                 357 
                 CD36 
                 NM_000072.3 
                 Endogenous 
               
               
                 358 
                 IL7 
                 NM_000880.2 
                 Endogenous 
               
               
                 359 
                 CBLL1 
                 NM_024814.3 
                 Endogenous 
               
               
                 360 
                 HVCN1 
                 NM_032369.3 
                 Endogenous 
               
               
                 361 
                 HMGB1 
                 NM_002128.4 
                 Endogenous 
               
               
                 362 
                 SIN3A 
                 NM_015477.2 
                 Endogenous 
               
               
                 363 
                 CASP3 
                 NM_032991.2 
                 Endogenous 
               
               
                 364 
                 BQ189294 
                 BQ189294.1 
                 Endogenous 
               
               
                 365 
                 NDRG2 
                 NM_016250.2 
                 Endogenous 
               
               
                 366 
                 BX400436 
                 BX400436.2 
                 Endogenous 
               
               
                 367 
                 IFNAR2 
                 NM_000874.3 
                 Endogenous 
               
               
                 368 
                 MS4A6A b 
                 NM_152851.2 
                 Endogenous 
               
               
                 369 
                 KLRC2 
                 NM_002260.3 
                 Endogenous 
               
               
                 370 
                 S100A12 b 
                 NM_005621.1 
                 Endogenous 
               
               
                 371 
                 ATM 
                 NM_000051.3 
                 Endogenous 
               
               
                 372 
                 NLRP3 
                 NM_001079821.2 
                 Endogenous 
               
               
                 373 
                 HAVCR2 
                 NM_032782.3 
                 Endogenous 
               
               
                 374 
                 C4B 
                 NM_001002029.3 
                 Endogenous 
               
               
                 375 
                 CTSW 
                 NM_001335.3 
                 Endogenous 
               
               
                 376 
                 TMEM170B 
                 NM_001100829.2 
                 Endogenous 
               
               
                 377 
                 EIF4ENIF1 
                 NM_019843.2 
                 Endogenous 
               
               
                 378 
                 CCL3 
                 NM_002983.2 
                 Endogenous 
               
               
                 379 
                 CHCHD3 
                 NM_017812.2 
                 Endogenous 
               
               
                 380 
                 CST7 
                 NM_003650.3 
                 Endogenous 
               
               
                 381 
                 SFRS15 
                 NM_020706.2 
                 Endogenous 
               
               
                 382 
                 STIP1 
                 NM_006819.2 
                 Endogenous 
               
               
                 383 
                 MPDU1 
                 NM_004870.3 
                 Endogenous 
               
               
                 384 
                 DHX16 b 
                 NM_001164239.1 
                 Endogenous 
               
               
                 385 
                 INTS4 
                 NM_033547.3 
                 Endogenous 
               
               
                 386 
                 USP16 
                 NM_001032410.1 
                 Endogenous 
               
               
                 387 
                 IFNAR1 
                 NM_000629.2 
                 Endogenous 
               
               
                 388 
                 ITCH 
                 NM_001257138.1 
                 Endogenous 
               
               
                 389 
                 FOXK2 
                 NM_004514.3 
                 Endogenous 
               
               
                 390 
                 LOC642812 
                 XR_036892.1 
                 Endogenous 
               
               
                 391 
                 KIAA1967 
                 NM_021174.5 
                 Endogenous 
               
               
                 392 
                 LOC440928 
                 XM_942885.1 
                 Endogenous 
               
               
                 393 
                 NDUFV2 
                 NM_021074.4 
                 Endogenous 
               
               
                 394 
                 IL4 
                 NM_000589.2 
                 Endogenous 
               
               
                 395 
                 CIAPIN1 
                 NM_020313.3 
                 Endogenous 
               
               
                 396 
                 CXCL2 
                 NM_002089.3 
                 Endogenous 
               
               
                 397 
                 TXN 
                 NM_003329.3 
                 Endogenous 
               
               
                 398 
                 PRG2 
                 NM_002728.4 
                 Endogenous 
               
               
                 399 
                 MS4A2 
                 NM_000139.3 
                 Endogenous 
               
               
                 400 
                 YPEL1 
                 NM_013313.4 
                 Endogenous 
               
               
                 401 
                 POLR2A 
                 NM_000937.4 
                 Endogenous 
               
               
                 402 
                 C19orf10 
                 NM_019107.3 
                 Endogenous 
               
               
                 403 
                 IGFBP7 
                 NM_001553.2 
                 Endogenous 
               
               
                 404 
                 ITGAE 
                 NM_002208.4 
                 Endogenous 
               
               
                 405 
                 CXCR5 b 
                 NM_001716.3 
                 Endogenous 
               
               
                 406 
                 BID 
                 NM_001196.2 
                 Endogenous 
               
               
                 407 
                 LOC100133273 
                 XR_039238.1 
                 Endogenous 
               
               
                 408 
                 FNBP1 
                 NM_015033.2 
                 Endogenous 
               
               
                 409 
                 IFNGR1 
                 NM_000416.1 
                 Endogenous 
               
               
                 410 
                 STAT6 
                 NM_003153.4 
                 Endogenous 
               
               
                 411 
                 CR2 
                 NM_001006658.2 
                 Endogenous 
               
               
                 412 
                 CCL3L3 
                 NM_001001437.3 
                 Endogenous 
               
               
                 413 
                 RFWD2 
                 NM_022457.6 
                 Endogenous 
               
               
                 414 
                 SP2 
                 NM_003110.5 
                 Endogenous 
               
               
                 415 
                 BAT2D1 
                 NM_015172.3 
                 Endogenous 
               
               
                 416 
                 CX3CL1 
                 NM_002996.3 
                 Endogenous 
               
               
                 417 
                 GPATCH3 
                 NM_022078.2 
                 Endogenous 
               
               
                 418 
                 CASP1 
                 NM_033294.3 
                 Endogenous 
               
               
                 419 
                 NAGK 
                 NM_017567.4 
                 Endogenous 
               
               
                 420 
                 IER5 
                 NM_016545.4 
                 Endogenous 
               
               
                 421 
                 PHLPP2 
                 NM_015020.3 
                 Endogenous 
               
               
                 422 
                 RPL31 
                 NM_000993.4 
                 Endogenous 
               
               
                 423 
                 SPEN 
                 NM_015001.2 
                 Endogenous 
               
               
                 424 
                 TMSB4X 
                 NM_021109.3 
                 Endogenous 
               
               
                 425 
                 IL8RB 
                 NM_001557.3 
                 Endogenous 
               
               
                 426 
                 XPC 
                 NR_027299.1 
                 Endogenous 
               
               
                 427 
                 SNX11 
                 NM_152244.1 
                 Endogenous 
               
               
                 428 
                 SPN 
                 NM_003123.3 
                 Endogenous 
               
               
                 429 
                 ANKHD1 
                 NM_017747.2 
                 Endogenous 
               
               
                 430 
                 CCR6 
                 NM_031409.2 
                 Endogenous 
               
               
                 431 
                 DZIP3 
                 NM_014648.3 
                 Endogenous 
               
               
                 432 
                 MRPL27 
                 NM_148571.1 
                 Endogenous 
               
               
                 433 
                 SREBF1 
                 NM_001005291.2 
                 Endogenous 
               
               
                 434 
                 CD14 
                 NM_000591.2 
                 Endogenous 
               
               
                 435 
                 TNFSF8 
                 NM_001244.3 
                 Endogenous 
               
               
                 436 
                 C3 
                 NM_000064.2 
                 Endogenous 
               
               
                 437 
                 FAM50B 
                 NM_012135.1 
                 Endogenous 
               
               
                 438 
                 RASSF5 
                 NM_182664.2 
                 Endogenous 
               
               
                 439 
                 BU743228 
                 BU743228.1 
                 Endogenous 
               
               
                 440 
                 NFATC1 
                 NM_172389.1 
                 Endogenous 
               
               
                 441 
                 DOCK5 
                 NM_024940.6 
                 Endogenous 
               
               
                 442 
                 PACS1 
                 NM_018026.3 
                 Endogenous 
               
               
                 443 
                 CYP1B1 
                 NM_000104.3 
                 Endogenous 
               
               
                 444 
                 CLIC3 
                 ILMN_1796423.1 
                 Endogenous 
               
               
                 445 
                 PSMA4 
                 NM_002789.3 
                 Endogenous 
               
               
                 446 
                 ZNF341 
                 NM_032819.4 
                 Endogenous 
               
               
                 447 
                 PRPF3 
                 NM_004698.2 
                 Endogenous 
               
               
                 448 
                 PSMA6 b 
                 NM_002791.2 
                 Endogenous 
               
               
                 449 
                 LOC648927 
                 XR_038906.2 
                 Endogenous 
               
               
                 450 
                 KCTD12 
                 NM_138444.3 
                 Endogenous 
               
               
                 451 
                 LOC440389 
                 XM_498648.3 
                 Endogenous 
               
               
                 452 
                 U2AF2 
                 NM_007279.2 
                 Endogenous 
               
               
                 453 
                 CLEC5A 
                 NM_013252.2 
                 Endogenous 
               
               
                 454 
                 PRRG4 
                 NM_024081.5 
                 Endogenous 
               
               
                 455 
                 TNFRSF9 
                 NM_001561.5 
                 Endogenous 
               
               
                 456 
                 NDUFB3 
                 NM_002491.2 
                 Endogenous 
               
               
                 457 
                 BCL6 
                 NM_001130845.1 
                 Endogenous 
               
               
                 458 
                 SGK1 
                 NM_005627.3 
                 Endogenous 
               
               
                 459 
                 CIP29 
                 NM_033082.3 
                 Endogenous 
               
               
                 460 
                 CD160 b 
                 NM_007053.2 
                 Endogenous 
               
               
                 461 
                 ARCN1 
                 NM_001655.4 
                 Endogenous 
               
               
                 462 
                 LOC151162 
                 NR_024275.1 
                 Endogenous 
               
               
                 463 
                 GPR65 
                 NM_003608.3 
                 Endogenous 
               
               
                 464 
                 CCR1 
                 NM_001295.2 
                 Endogenous 
               
               
                 465 
                 TFCP2 
                 NM_005653.4 
                 Endogenous 
               
               
                 466 
                 SGK 
                 NM_005627.3 
                 Endogenous 
               
               
                 467 
                 RNF214 
                 NM_207343.3 
                 Endogenous 
               
               
                 468 
                 TMC8 
                 NM_152468.4 
                 Endogenous 
               
               
                 469 
                 RBM14 
                 NM_006328.3 
                 Endogenous 
               
               
                 470 
                 USP34 
                 NM_014709.3 
                 Endogenous 
               
               
                 471 
                 BACH2 
                 NM_021813.3 
                 Endogenous 
               
               
                 472 
                 LILRA5 
                 NM_021250.3 
                 Endogenous 
               
               
                 473 
                 C5orf21 
                 NM_032042.5 
                 Endogenous 
               
               
                 474 
                 LOC441073 
                 XR_018937.2 
                 Endogenous 
               
               
                 475 
                 TAX1BP1 
                 NM_001079864.2 
                 Endogenous 
               
               
                 476 
                 TNFSF13 
                 NM_003808.3 
                 Endogenous 
               
               
                 477 
                 PIM2 
                 NM_006875.3 
                 Endogenous 
               
               
                 478 
                 RNF19B 
                 NM_153341.3 
                 Endogenous 
               
               
                 479 
                 EPHX2 
                 NM_001979.5 
                 Endogenous 
               
               
                 480 
                 LILRA5 b 
                 NM_181879.2 
                 Endogenous 
               
               
                 481 
                 ABCF1 
                 NM_001025091.1 
                 Endogenous 
               
               
                 482 
                 C4orf27 
                 NM_017867.2 
                 Endogenous 
               
               
                 483 
                 PSMB7 
                 NM_002799.2 
                 Endogenous 
               
               
                 484 
                 LPCAT4 
                 NM_153613.2 
                 Endogenous 
               
               
                 485 
                 TRIM21 
                 NM_003141.3 
                 Endogenous 
               
               
                 486 
                 LOC728835 
                 XM_001133190.1 
                 Endogenous 
               
               
                 487 
                 NFKB1 
                 NM_003998.3 
                 Endogenous 
               
               
                 488 
                 CR2 b 
                 NM_001006658.1 
                 Endogenous 
               
               
                 489 
                 HMGB2 
                 NM_002129.3 
                 Endogenous 
               
               
                 490 
                 IL1B 
                 NM_000576.2 
                 Endogenous 
               
               
                 491 
                 C20orf52 
                 NM_080748.2 
                 Endogenous 
               
               
                 492 
                 DNAJB6 
                 NM_058246.3 
                 Endogenous 
               
               
                 493 
                 PFDN5 
                 NM_145897.2 
                 Endogenous 
               
               
                 494 
                 RPS6 
                 NM_001010.2 
                 Endogenous 
               
               
                 495 
                 LEF1 
                 NM_016269.4 
                 Endogenous 
               
               
                 496 
                 DKFZp761P0423 
                 XM_291277.4 
                 Endogenous 
               
               
                 497 
                 LOC647340 
                 XR_018104.1 
                 Endogenous 
               
               
                 498 
                 FTHL16 
                 XR_041433.1 
                 Endogenous 
               
               
                 499 
                 COX6C 
                 NM_004374.2 
                 Endogenous 
               
               
                 500 
                 BCL10 
                 NM_003921.2 
                 Endogenous 
               
               
                 501 
                 CD48 
                 NM_001778.2 
                 Endogenous 
               
               
                 502 
                 ZMIZ1 
                 NM_020338.3 
                 Endogenous 
               
               
                 503 
                 GZMH 
                 NM_033423.4 
                 Endogenous 
               
               
                 504 
                 TRRAP 
                 NM_003496.3 
                 Endogenous 
               
               
                 505 
                 SH2D3C 
                 NM_170600.2 
                 Endogenous 
               
               
                 506 
                 UBC 
                 NM_021009.3 
                 Endogenous 
               
               
                 507 
                 TXNDC17 
                 NM_032731.3 
                 Endogenous 
               
               
                 508 
                 ATP5J2 
                 NM_004889.3 
                 Endogenous 
               
               
                 509 
                 KIAA1267 
                 NM_015443.3 
                 Endogenous 
               
               
                 510 
                 RFX1 
                 NM_002918.4 
                 Endogenous 
               
               
                 511 
                 WDR1 
                 NM_005112.4 
                 Endogenous 
               
               
                 512 
                 LOC100129697 
                 XM_001732822.2 
                 Endogenous 
               
               
                 513 
                 TOMM7 
                 NM_019059.2 
                 Endogenous 
               
               
                 514 
                 ARHGAP26 
                 NM_015071.4 
                 Endogenous 
               
               
                 515 
                 HSPA6 
                 NM_002155.4 
                 Endogenous 
               
               
                 516 
                 FLJ10357 
                 NM_018071.4 
                 Endogenous 
               
               
                 517 
                 ITGAL 
                 NM_002209.2 
                 Endogenous 
               
               
                 518 
                 BX089765 
                 BX089765.1 
                 Endogenous 
               
               
                 519 
                 RERE 
                 NM_001042682.1 
                 Endogenous 
               
               
                 520 
                 C15orf39 
                 NM_015492.4 
                 Endogenous 
               
               
                 521 
                 BX436458 
                 BX436458.2 
                 Endogenous 
               
               
                 522 
                 RWDD1 
                 NM_001007464.2 
                 Endogenous 
               
               
                 523 
                 TMBIM6 
                 NM_003217.2 
                 Endogenous 
               
               
                 524 
                 SLC6A6 
                 NM_003043.5 
                 Endogenous 
               
               
                 525 
                 KIAA0174 
                 NM_014761.3 
                 Endogenous 
               
               
                 526 
                 IL16 
                 NM_004513.4 
                 Endogenous 
               
               
                 527 
                 EGLN1 
                 NM_022051.1 
                 Endogenous 
               
               
                 528 
                 LOC391126 
                 XR_017684.2 
                 Endogenous 
               
               
                 529 
                 TAPBP 
                 NM_003190.4 
                 Endogenous 
               
               
                 530 
                 NUMB 
                 NM_001005744.1 
                 Endogenous 
               
               
                 531 
                 CENTD2 
                 NM_001040118.2 
                 Endogenous 
               
               
                 532 
                 CLSTN1 
                 NM_001009566.2 
                 Endogenous 
               
               
                 533 
                 PSMA4 b 
                 NM_002789.4 
                 Endogenous 
               
               
                 534 
                 LOC648000 
                 XM_371757.4 
                 Endogenous 
               
               
                 535 
                 COX7C 
                 NM_001867.2 
                 Endogenous 
               
               
                 536 
                 PIK3CD 
                 NM_005026.3 
                 Endogenous 
               
               
                 537 
                 UQCRQ 
                 NM_014402.4 
                 Endogenous 
               
               
                 538 
                 IDS 
                 NM_006123.4 
                 Endogenous 
               
               
                 539 
                 C19orf59 
                 NM_174918.2 
                 Endogenous 
               
               
                 540 
                 MYL12A 
                 NM_006471.3 
                 Housekeeping 
               
               
                 541 
                 EIF2B4 
                 NM_015636.3 
                 Housekeeping 
               
               
                 542 
                 DGUOK b 
                 NM_080916.2 
                 Housekeeping 
               
               
                 543 
                 PSMC1 
                 NM_002802.2 
                 Housekeeping 
               
               
                 544 
                 CHFR 
                 NM_018223.2 
                 Housekeeping 
               
               
                 545 
                 ARPC2 
                 NM_005731.2 
                 Housekeeping 
               
               
                 546 
                 ATP5B 
                 NM_001686.3 
                 Housekeeping 
               
               
                 547 
                 RPL3 
                 NM_001033853.1 
                 Housekeeping 
               
               
                 548 
                 ZNF143 
                 NM_003442.5 
                 Housekeeping 
               
               
                 549 
                 PSMD7 
                 NM_002811.4 
                 Housekeeping 
               
               
                 550 
                 TBP 
                 NM_003194.4 
                 Housekeeping 
               
               
                 551 
                 DHX16 
                 NM_003587.4 
                 Housekeeping 
               
               
                 552 
                 TUG1 
                 NR_002323.2 
                 Housekeeping 
               
               
                 553 
                 GUSB 
                 NM_000181.3 
                 Housekeeping 
               
               
                 554 
                 HDAC3 
                 NM_003883.3 
                 Housekeeping 
               
               
                 555 
                 SDHA 
                 NM_004168.3 
                 Housekeeping 
               
               
                 556 
                 PGK1 
                 NM_000291.3 
                 Housekeeping 
               
               
                 557 
                 STAMBP 
                 NM_006463.4 
                 Housekeeping 
               
               
                 558 
                 MTCH1 
                 NM_014341.2 
                 Housekeeping 
               
               
                 559 
                 TUBB 
                 NM_178014.2 
                 Housekeeping 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                 TABLE II 
               
               
                   
               
               
                 Rank 
                 Sequence ID# 
                 Gene 
                 Class Name 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 1 
                 TPR 
                 NM_003292.2 
                 Endogenous 
               
               
                 2 
                 DNAJB1 
                 NM_006145.2 
                 Endogenous 
               
               
                 3 
                 PDCD10 
                 NM_145859.1 
                 Endogenous 
               
               
                 4 
                 PSMB7 
                 NM_002799.2 
                 Endogenous 
               
               
                 5 
                 MERTK 
                 NM_006343.2 
                 Endogenous 
               
               
                 6 
                 AFTPH 
                 NM_017657.4 
                 Endogenous 
               
               
                 7 
                 BCOR 
                 NM_017745.5 
                 Endogenous 
               
               
                 8 
                 RASSF5 
                 NM_182664.2 
                 Endogenous 
               
               
                 9 
                 SNX11 
                 NM_152244.1 
                 Endogenous 
               
               
                 10 
                 ANP32B 
                 NM_006401.2 
                 Endogenous 
               
               
                 11 
                 C4B 
                 NM_001002029.3 
                 Endogenous 
               
               
                 12 
                 NME1-NME2 
                 NM_001018136.2 
                 Endogenous 
               
               
                 13 
                 DGUOK 
                 NM_080916.2 
                 Endogenous 
               
               
                 14 
                 CYP1B1 
                 NM_000104.3 
                 Endogenous 
               
               
                 15 
                 MPDU1 
                 NM_004870.3 
                 Endogenous 
               
               
                 16 
                 MED16 
                 NM_005481.2 
                 Endogenous 
               
               
                 17 
                 FAM179A 
                 NM_199280.2 
                 Endogenous 
               
               
                 18 
                 CPPED1 
                 NM_018340.2 
                 Endogenous 
               
               
                 19 
                 LOC648927 
                 XR_038906.2 
                 Endogenous 
               
               
                 20 
                 ANKHD1 
                 NM_017747.2 
                 Endogenous 
               
               
                 21 
                 CN312986 
                 CN312986.1 
                 Endogenous 
               
               
                 22 
                 PHCA 
                 NM_018367.6 
                 Endogenous 
               
               
                 23 
                 CD1A 
                 NM_001763.2 
                 Endogenous 
               
               
                 24 
                 NCOA5 
                 NM_020967.2 
                 Endogenous 
               
               
                 25 
                 SLC6A12 
                 NM_003044.4 
                 Endogenous 
               
               
                 26 
                 LOC728533 
                 XR_015610.3 
                 Endogenous 
               
               
                 27 
                 TRAF3IP2 
                 NM_147686.3 
                 Endogenous 
               
               
                 28 
                 TBCE 
                 NM_001079515.2 
                 Endogenous 
               
               
                 29 
                 CCT6A 
                 NM_001762.3 
                 Endogenous 
               
               
                 30 
                 P2RY5 
                 NM_005767.5 
                 Endogenous 
               
               
                 31 
                 RNASE2 
                 NM_002934.2 
                 Endogenous 
               
               
                 32 
                 CLN8 
                 NM_018941.3 
                 Endogenous 
               
               
                 33 
                 REPS1 
                 NM_001128617.2 
                 Endogenous 
               
               
                 34 
                 TPT1 
                 NM_003295.3 
                 Endogenous 
               
               
                 35 
                 LOC100129022 
                 XM_001716591.1 
                 Endogenous 
               
               
                 36 
                 KLRC1 
                 NM_002259.3 
                 Endogenous 
               
               
                 37 
                 AZI2 
                 NM_022461.4 
                 Endogenous 
               
               
                 38 
                 FAM193A 
                 NM_003704.3 
                 Endogenous 
               
               
                 39 
                 PLAC8 
                 NM_001130715.1 
                 Endogenous 
               
               
                 40 
                 LDHA 
                 NM_001165416.1 
                 Endogenous 
               
               
                 41 
                 GPATCH3 
                 NM_022078.2 
                 Endogenous 
               
               
                 42 
                 RBM14 
                 NM_006328.3 
                 Endogenous 
               
               
                 43 
                 KYNU 
                 NM_001032998.1 
                 Endogenous 
               
               
                 44 
                 PPP2R5C 
                 NM_178588.1 
                 Endogenous 
               
               
                 45 
                 S100A12 b 
                 NM_005621.1 
                 Endogenous 
               
               
                 46 
                 SFMBT1 
                 NM_001005158.2 
                 Endogenous 
               
               
                 47 
                 CCR6 
                 NM_031409.2 
                 Endogenous 
               
               
                 48 
                 TRIM39 
                 NM_021253.3 
                 Endogenous 
               
               
                 49 
                 AK126342 
                 AK126342.1 
                 Endogenous 
               
               
                 50 
                 SLC45A3 
                 NM_033102.2 
                 Endogenous 
               
               
                 51 
                 IL4 
                 NM_000589.2 
                 Endogenous 
               
               
                 52 
                 UBE2I 
                 NM_194259.2 
                 Endogenous 
               
               
                 53 
                 PRPF3 
                 NM_004698.2 
                 Endogenous 
               
               
                 54 
                 NDUFB3 
                 NM_002491.2 
                 Endogenous 
               
               
                 55 
                 CRKL 
                 NM_005207.3 
                 Endogenous 
               
               
                 56 
                 IDO1 
                 NM_002164.3 
                 Endogenous 
               
               
                 57 
                 PUM1 
                 NM_001020658.1 
                 Endogenous 
               
               
                 58 
                 BCL10 
                 NM_003921.2 
                 Endogenous 
               
               
                 59 
                 TMBIM6 
                 NM_003217.2 
                 Endogenous 
               
               
                 60 
                 C17orf51 
                 XM_944416.1 
                 Endogenous 
               
               
                 61 
                 BANP 
                 NM_079837.2 
                 Endogenous 
               
               
                 62 
                 HAVCR2 
                 NM_032782.3 
                 Endogenous 
               
               
                 63 
                 BAG3 
                 NM_004281.3 
                 Endogenous 
               
               
                 64 
                 DBI 
                 NM_001079862.1 
                 Endogenous 
               
               
                 65 
                 C4orf27 
                 NM_017867.2 
                 Endogenous 
               
               
                 66 
                 TSC1 
                 NM_000368.4 
                 Endogenous 
               
               
                 67 
                 LPCAT4 
                 NM_153613.2 
                 Endogenous 
               
               
                 68 
                 SAMSN1 
                 NM_022136.3 
                 Endogenous 
               
               
                 69 
                 SNORA56 
                 NR_002984.1 
                 Endogenous 
               
               
                 70 
                 ARG1 
                 NM_000045.3 
                 Endogenous 
               
               
                 71 
                 IL1R2 
                 NM_173343.1 
                 Endogenous 
               
               
                 72 
                 CCND3 
                 NM_001760.2 
                 Endogenous 
               
               
                 73 
                 USP9Y 
                 NM_004654.3 
                 Endogenous 
               
               
                 74 
                 ATP2C1 
                 NM_014382.3 
                 Endogenous 
               
               
                 75 
                 PSMB1 
                 NM_002793.3 
                 Endogenous 
               
               
                 76 
                 NDUFAF2 
                 NM_174889.4 
                 Endogenous 
               
               
                 77 
                 VPS37C 
                 NM_017966.4 
                 Endogenous 
               
               
                 78 
                 HAT1 
                 NM_003642.3 
                 Endogenous 
               
               
                 79 
                 LOC732371 
                 XM_001133019.1 
                 Endogenous 
               
               
                 80 
                 LOC148137 
                 NM_144692.1 
                 Endogenous 
               
               
                 81 
                 CCR1 
                 NM_001295.2 
                 Endogenous 
               
               
                 82 
                 CCDC97 
                 NM_052848.1 
                 Endogenous 
               
               
                 83 
                 PPP6C 
                 NM_002721.4 
                 Endogenous 
               
               
                 84 
                 GPI 
                 NM_000175.2 
                 Endogenous 
               
               
                 85 
                 PIM2 
                 NM_006875.3 
                 Endogenous 
               
               
                 86 
                 STAT6 
                 NM_003153.4 
                 Endogenous 
               
               
                 87 
                 BATF 
                 NM_006399.3 
                 Endogenous 
               
               
                 88 
                 EIF4ENIF1 
                 NM_019843.2 
                 Endogenous 
               
               
                 89 
                 HSP90AB1 
                 NM_007355.3 
                 Endogenous 
               
               
                 90 
                 U2AF2 
                 NM_007279.2 
                 Endogenous 
               
               
                 91 
                 CYBB 
                 NM_000397.3 
                 Endogenous 
               
               
                 92 
                 WDR1 
                 NM_005112.4 
                 Endogenous 
               
               
                 93 
                 PSMB8 
                 NM_004159.4 
                 Endogenous 
               
               
                 94 
                 TBC1D12 
                 NM_015188.1 
                 Endogenous 
               
               
                 95 
                 LOC648000 
                 XM_371757.4 
                 Endogenous 
               
               
                 96 
                 XCL2 
                 NM_003175.3 
                 Endogenous 
               
               
                 97 
                 PTGDR 
                 NM_000953.2 
                 Endogenous 
               
               
                 98 
                 ACSL5 
                 NM_203379.1 
                 Endogenous 
               
               
                 99 
                 CASP1 
                 NM_033294.3 
                 Endogenous 
               
               
                 100 
                 UBTF 
                 NM_001076683.1 
                 Endogenous 
               
               
                   
               
            
           
         
       
     
     In one embodiment, a novel gene expression profile or signature can identify and distinguish patients having cancerous tumors from patients having benign nodules. See for example the genes identified in Table I and Table II which may form a suitable gene expression profile. In another embodiment, a portion of the genes of Table I form a suitable profile. In yet another embodiment, a portion of the genes of Table II form a suitable profile. As discussed herein, these profiles are used to distinguish between cancerous and non-cancerous tumors by generating a discriminant score based on differences in gene expression profiles as exemplified below. The validity of these signatures was established on samples collected at different locations by different groups in a cohort of patients with undiagnosed lung nodules. See Example 7 and  FIGS.  2 A- 2 B  and  FIG.  6   . The lung cancer signatures or gene expression profiles identified herein (i.e., Table I or Table II) may be further optimized to reduce the numbers of gene expression products necessary and increase accuracy of diagnosis. 
     In one embodiment, the composition includes 10 to 559 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In another embodiment, the composition includes 10 to 100 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table II. In another embodiment, the composition includes 10 to 559 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In another embodiment, the composition includes 10 to 100 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table II. In another embodiment, the composition includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 547, 548, 549, 550, 551, 552, 553, 554, 555, 556, 557, 558, or 559 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In another embodiment, the composition includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table II. In one embodiment, the composition includes at least 3 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 5 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 10 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 15 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 20 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 25 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 30 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 35 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 40 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 45 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 50 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 55 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 60 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 65 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 70 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 75 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 80 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 85 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 90 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 95 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 100 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I or Table II. In one embodiment, the composition includes at least 150 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In one embodiment, the composition includes at least 200 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In one embodiment, the composition includes at least 250 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In one embodiment, the composition includes at least 300 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In one embodiment, the composition includes at least 350 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In one embodiment, the composition includes at least 400 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In one embodiment, the composition includes at least 450 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In one embodiment, the composition includes at least 500 polynucleotides or oligonucleotides or ligands, wherein each polynucleotide or oligonucleotide or ligand hybridizes to a different gene, gene fragment, gene transcript or expression product in a sample selected from the genes of Table I. In one embodiment, the composition includes polynucleotides or oligonucleotides or ligands capable of hybridizing to each different gene, gene fragment, gene transcript or expression product listed in Table I. In another embodiment, the composition includes polynucleotides or oligonucleotides or ligands capable of hybridizing to each different gene, gene fragment, gene transcript or expression product listed in Table II. 
     In yet another embodiment, the expression profile is formed by the first 3 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 5 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 10 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 15 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 20 genes in rank order of Table I or Table II. In another embodiment, the expression profile is formed by the first 25 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 30 genes in rank order of Table I or Table II. In another embodiment, the expression profile is formed by the first 35 genes in rank order of Table I or Table II. In another embodiment, the expression profile is formed by the first 40 genes in rank order of Table I or Table II. In another embodiment, the expression profile is formed by the first 45 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 50 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 55 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 60 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 65 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 70 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 75 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 80 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 85 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 90 genes in rank order of Table I or Table II. In yet another embodiment, the expression profile is formed by the first 95 genes in rank order of Table I or Table II. In another embodiment, the expression profile is formed by the first 100 genes in rank order of Table I or Table II. In another embodiment, the expression profile is formed by the first 150 genes in rank order of Table I. In another embodiment, the expression profile is formed by the first 200 genes in rank order of Table I. In another embodiment, the expression profile is formed by the first 250 genes in rank order of Table I. In another embodiment, the expression profile is formed by the first 300 genes in rank order of Table I. In another embodiment, the expression profile is formed by the first 350 genes in rank order of Table I. In another embodiment, the expression profile is formed by the first 400 genes in rank order of Table I. In yet another embodiment, the expression profile is formed by the first 539 genes in rank order of Table I. 
     As discussed below, the compositions described herein can be used with the gene expression profiling methods which are known in the art. Thus, the compositions can be adapted accordingly to suit the method for which they are intended to be used. In one embodiment, at least one polynucleotide or oligonucleotide or ligand is attached to a detectable label. In certain embodiments, each polynucleotide or oligonucleotide is attached to a different detectable label, each capable of being detected independently. Such reagents are useful in assays such as the nCounter, as described below. 
     In another embodiment, the composition comprises a capture oligonucleotide or ligand, which hybridizes to at least one polynucleotide or oligonucleotide or ligand. In one embodiment, such capture oligonucleotide or ligand may include a nucleic acid sequence which is specific for a portion of the oligonucleotide or polynucleotide or ligand which is specific for the gene of interest. The capture ligand may be a peptide or polypeptide which is specific for the ligand to the gene of interest. In one embodiment, the capture ligand is an antibody, as in a sandwich ELISA. 
     The capture oligonucleotide also includes a moiety which allows for binding with a substrate. Such substrate includes, without limitation, a plate, bead, slide, well, chip or chamber. In one embodiment, the composition includes a capture oligonucleotide for each different polynucleotide or oligonucleotide which is specific to a gene of interest. Each capture oligonucleotide may contain the same moiety which allows for binding with the same substrate. In one embodiment, the binding moiety is biotin. 
     Thus, a composition for such diagnosis or evaluation in a mammalian subject as described herein can be a kit or a reagent. For example, one embodiment of a composition includes a substrate upon which the ligands used to detect and quantitate mRNA are immobilized. The reagent, in one embodiment, is an amplification nucleic acid primer (such as an RNA primer) or primer pair that amplifies and detects a nucleic acid sequence of the mRNA. In another embodiment, the reagent is a polynucleotide probe that hybridizes to the target sequence. In another embodiment, the target sequences are illustrated in Table III. In another embodiment, the reagent is an antibody or fragment of an antibody. The reagent can include multiple said primers, probes or antibodies, each specific for at least one gene, gene fragment or expression product of Table I or Table II. Optionally, the reagent can be associated with a conventional detectable label. 
     In another embodiment, the composition is a kit containing the relevant multiple polynucleotides or oligonucleotide probes or ligands, optional detectable labels for same, immobilization substrates, optional substrates for enzymatic labels, as well as other laboratory items. In still another embodiment, at least one polynucleotide or oligonucleotide or ligand is associated with a detectable label. In certain embodiments, the reagent is immobilized on a substrate. Exemplary substrates include a microarray, chip, microfluidics card, or chamber. 
     In one embodiment, the composition is a kit designed for use with the nCounter Nanostring system, as further discussed below. 
     II. GENE EXPRESSION PROFILING METHODS 
     Methods of gene expression profiling that were used in generating the profiles useful in the compositions and methods described herein or in performing the diagnostic steps using the compositions described herein are known and well summarized in U.S. Pat. No. 7,081,340. Such methods of gene expression profiling include methods based on hybridization analysis of polynucleotides, methods based on sequencing of polynucleotides, and proteomics-based methods. The most commonly used methods known in the art for the quantification of mRNA expression in a sample include northern blotting and in situ hybridization; RNAse protection assays; nCounter® Analysis; and PCR-based methods, such as RT-PCR. Alternatively, antibodies may be employed that can recognize specific duplexes, including DNA duplexes, RNA duplexes, and DNA-RNA hybrid duplexes or DNA-protein duplexes. Representative methods for sequencing-based gene expression analysis include Serial Analysis of Gene Expression (SAGE), and gene expression analysis by massively parallel signature sequencing (MPSS). 
     In certain embodiments, the compositions described herein are adapted for use in the methods of gene expression profiling described herein, and those known in the art. 
     A. Patient Sample 
     The “sample” or “biological sample” as used herein means any biological fluid or tissue that contains immune cells and/or cancer cells. In one embodiment, a suitable sample is whole blood. In another embodiment, the sample may be venous blood. In another embodiment, the sample may be arterial blood. In another embodiment, a suitable sample for use in the methods described herein includes peripheral blood, more specifically peripheral blood mononuclear cells. Other useful biological samples include, without limitation, plasma or serum. In still other embodiment, the sample is saliva, urine, synovial fluid, bone marrow, cerebrospinal fluid, vaginal mucus, cervical mucus, nasal secretions, sputum, semen, amniotic fluid, bronchoalveolar lavage fluid, and other cellular exudates from a subject suspected of having a lung disease. Such samples may further be diluted with saline, buffer or a physiologically acceptable diluent. Alternatively, such samples are concentrated by conventional means. It should be understood that the use or reference throughout this specification to any one biological sample is exemplary only. For example, where in the specification the sample is referred to as whole blood, it is understood that other samples, e.g., serum, plasma, etc., may also be employed in another embodiment. 
     In one embodiment, the biological sample is whole blood, and the method employs the PaxGene Blood RNA Workflow system (Qiagen). That system involves blood collection (e.g., single blood draws) and RNA stabilization, followed by transport and storage, followed by purification of Total RNA and Molecular RNA testing. This system provides immediate RNA stabilization and consistent blood draw volumes. The blood can be drawn at a physician&#39;s office or clinic, and the specimen transported and stored in the same tube. Short term RNA stability is 3 days at between 18-25° C. or 5 days at between 2-8° C. Long term RNA stability is 4 years at −20 to −70° C. This sample collection system enables the user to reliably obtain data on gene expression in whole blood. In one embodiment, the biological sample is whole blood. While the PAXgene system has more noise than the use of PBMC as a biological sample source, the benefits of PAXgene sample collection outweighs the problems. Noise can be subtracted bioinformatically by the person of skill in the art. 
     In one embodiment, the biological samples may be collected using the proprietary PaxGene Blood RNA System (PreAnalytiX, a Qiagen, BD company). The PAXgene Blood RNA System comprises two integrated components: PAXgene Blood RNA Tube and the PAXgene Blood RNA Kit. Blood samples are drawn directly into PAXgene Blood RNA Tubes via standard phlebotomy technique. These tubes contain a proprietary reagent that immediately stabilizes intracellular RNA, minimizing the ex-vivo degradation or up-regulation of RNA transcripts. The ability to eliminate freezing, batch samples, and to minimize the urgency to process samples following collection, greatly enhances lab efficiency and reduces costs. Thereafter, the miRNA is detected and/or measured using a variety of assays. 
     B. Nanostring Analysis 
     A sensitive and flexible quantitative method that is suitable for use with the compositions and methods described herein is the nCounter® Analysis system (NanoString Technologies, Inc., Seattle Wash.). The nCounter Analysis System utilizes a digital color-coded barcode technology that is based on direct multiplexed measurement of gene expression and offers high levels of precision and sensitivity (&lt;1 copy per cell). The technology uses molecular “barcodes” and single molecule imaging to detect and count hundreds of unique transcripts in a single reaction. Each color-coded barcode is attached to a single target-specific probe (i.e., polynucleotide, oligonucleotide or ligand) corresponding to a gene of interest, i.e., a gene of Table I. Mixed together with controls, they form a multiplexed CodeSet. In one embodiment, the CodeSet includes all 559 genes of Table I. In another embodiment, the CodeSet includes all 100 genes of Table II. In another embodiment, the CodeSet includes at least 3 genes of Table I or Table II. In another embodiment, the CodeSet includes at least 5 genes of Table I or Table II. In another embodiment, the CodeSet includes at least 10 genes of Table I or Table II. In another embodiment, the CodeSet includes at least 15 genes of Table I or Table II. In another embodiment, the CodeSet includes at least 20 genes of Table I or Table II. In another embodiment, the CodeSet includes at least 25 genes of Table I or Table II. In another embodiment, the CodeSet includes at least 30 genes of Table I or Table II. In yet another embodiment, the CodeSet includes at least 40 genes of Table I or Table II. In yet another embodiment, the CodeSet includes at least 50 genes of Table I or Table II. In another embodiment, the CodeSet includes at least 60 genes of Table I or Table II. In another embodiment, the CodeSet includes at least 70 genes of Table I or Table II. In yet another embodiment, the CodeSet includes at least 80 genes of Table I or Table II. In yet another embodiment, the CodeSet includes at least 90 genes of Table I or Table II. In another embodiment, the CodeSet includes at least 100 genes of Table I. In another embodiment, the CodeSet includes at least 200 genes of Table I. In another embodiment, the CodeSet includes at least 300 genes of Table I. In yet another embodiment, the CodeSet includes at least 400 genes of Table I. In yet another embodiment, the CodeSet includes at least 500 genes of Table I. In yet another embodiment, the CodeSet is formed by the first 539 genes in rank order of Table I. In yet another embodiment, the CodeSet includes any subset of genes of Table I, as described herein. In another embodiment, the CodeSet includes any subset of genes of Table II, as described herein. 
     The NanoString platform employs two ˜50 base probes per mRNA that hybridizes in solution. The Reporter Probe carries the signal; the Capture Probe allows the complex to be immobilized for data collection. The probes are mixed with the patient sample. After hybridization, the excess probes are removed and the probe/target complexes aligned and immobilized to a substrate, e.g., in the nCounter Cartridge. 
     The target sequences utilized in the Examples below for each of the genes of Table I and Table II are shown in Table III below, and are reproduced in the sequence listing. These sequences are portions of the published sequences of these genes. Suitable alternatives may be readily designed by one of skill in the art. 
     Sample Cartridges are placed in the Digital Analyzer for data collection. Color codes on the surface of the cartridge are counted and tabulated for each target molecule. 
     A benefit of the use of the NanoString nCounter system is that no amplification of mRNA is necessary in order to perform the detection and quantification. However, in alternate embodiments, other suitable quantitative methods are used. See, e.g., Geiss et al, Direct multiplexed measurement of gene expression with color-coded probe pairs, Nat Biotechnol. 2008 March; 26(3):317-25. doi: 10.1038/nbt1385. Epub 2008 Feb. 17, which is incorporated herein by reference in its entirety. 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE III 
               
               
                   
               
               
                   
                 Se- 
                   
                   
                   
               
               
                   
                 quence 
                   
                 Posi- 
                   
               
               
                   
                 ID# 
                 Gene 
                 tion 
                 Target Sequence 
               
               
                   
               
             
            
               
                   1 
                 ABCA5 
                 NM_018672.4 
                  6839- 
                 AAGGAAGACTGTGTGTAGAATCT 
               
               
                   
                   
                   
                  6938 
                 TACGTAATAGTCTGATTCTTTGA 
               
               
                   
                   
                   
                   
                 CTCTGTGGCTAGAATGACAGTTA 
               
               
                   
                   
                   
                   
                 TCTATGGAGGTGGTAGAATTAAG 
               
               
                   
                   
                   
                   
                 CCATACCT 
               
               
                   
               
               
                   2 
                 ABCF1 
                 NM_00102509 
                  2875- 
                 CCTAAACAAACAAGAGGTGACC 
               
               
                   
                   
                 1.1 
                  2974  
                 ACCTTATTGTGAGGTTCCATCCA 
               
               
                   
                   
                   
                   
                 GCCAAGTTTATGTGGCCTATTGT 
               
               
                   
                   
                   
                   
                 CTCAGGACTCTCATCACTCAGAA 
               
               
                   
                   
                   
                   
                 GCCTGCCTC 
               
               
                   
               
               
                   3 
                 ACAA2 
                 NM_006111.2 
                  1605- 
                 CTCACTGTGACCCATCCTTACTC 
               
               
                   
                   
                   
                  1704 
                 TACTTGGCCAGGCCACAGTAAAA 
               
               
                   
                   
                   
                   
                 CAAGTGACCTTCAGAGCAGCTGC 
               
               
                   
                   
                   
                   
                 CACAACTGGCCATGCCCTGCCAT 
               
               
                   
                   
                   
                   
                 TGAAACAG 
               
               
                   
               
               
                   4 
                 PHCA 
                 NM_018367.6 
                  3324- 
                 AGCCAATAGTGATTTGTTTGCAT 
               
               
                   
                   
                   
                  3423 
                 ATCACCTAATGTGAAAAGTGCTC 
               
               
                   
                   
                   
                   
                 ATCTGTGAACTCTACAGCAAATT 
               
               
                   
                   
                   
                   
                 ATATTTTAGAAAATACTTTGTGA 
               
               
                   
                   
                   
                   
                 GGCCGGGC 
               
               
                   
               
               
                   5 
                 ACSL5 
                 NM_203379.1 
                  2701- 
                 CTATCACTCATGTCAATCATATC 
               
               
                   
                   
                   
                  2800 
                 TATGAGACAAATGTCTCCGATGC 
               
               
                   
                   
                   
                   
                 TCTTCTGCGTAAATTAAATTGTG 
               
               
                   
                   
                   
                   
                 TACTGAAGGGAAAAGTTTGATCA 
               
               
                   
                   
                   
                   
                 TACCAAAC 
               
               
                   
               
               
                   6 
                 CABC1 
                 NM_020247.4 
                  2536- 
                 TTCTAGAGTGAGATTTGTGTTTT 
               
               
                   
                   
                   
                  2635 
                 CTGCCCTTTTCCTCTCCAGCCGA 
               
               
                   
                   
                   
                   
                 TGGGCTGGAGCTGGGAGAGGTGC 
               
               
                   
                   
                   
                   
                 TGAGCTAACAGTGCCAACAAGT 
               
               
                   
                   
                   
                   
                 GCTCCTTAA 
               
               
                   
               
               
                   7 
                 CD97 
                 NM_078481.3 
                  3186- 
                 GCCAGTACTCGGGACAGACTAA 
               
               
                   
                   
                   
                  3285 
                 GGGCGCTTGTCCCATCCTGGACT 
               
               
                   
                   
                   
                   
                 TTTCCTCTCATGTCTTTGCTGCA 
               
               
                   
                   
                   
                   
                 GAACTGAAGAGACTAGGCGCTGG 
               
               
                   
                   
                   
                   
                 GGCTCAGCT 
               
               
                   
               
               
                   8 
                 AFTPH 
                 NM_017657.4 
                  2741- 
                 CTACCACCCGTCCAGTTTGACTG 
               
               
                   
                   
                   
                  2840 
                 GAGTAGCAGTGGCCTTACTAACC 
               
               
                   
                   
                   
                   
                 CTTTAGATGGTGTGGATCCGGAG 
               
               
                   
                   
                   
                   
                 TTGTATGAGTTAACAACTTCTAA 
               
               
                   
                   
                   
                   
                 GCTGGAAA 
               
               
                   
               
               
                   9 
                 AHCYL1 
                 NM_00124267 
                  2401- 
                 CTACCCGGCAGGTAGGTTAGATG 
               
               
                   
                   
                 6.1 
                  2500 
                 TGGGTGGTGCATGTTAATTTCCC 
               
               
                   
                   
                   
                   
                 TTAGAAGTTCCAAGCCCTGTTTC 
               
               
                   
                   
                   
                   
                 CTGCGTAAAGGTGGTATGTCCAG 
               
               
                   
                   
                   
                   
                 TTCAGAGA 
               
               
                   
               
               
                  10 
                 AK 
                 AK026725.1 
                  1869- 
                 AATGAAATTACTGTAGAGTCAGC 
               
               
                   
                 026725 
                   
                  1968 
                 AAAGAAGTAGAGAAGAAAAAAC 
               
               
                   
                   
                   
                   
                 ACCAAGAATGAGGAGAACCTAG 
               
               
                   
                   
                   
                   
                 CAAGGGCAGGCTTTTGGAAGCA 
               
               
                   
                   
                   
                   
                 AGAGGTAGATA 
               
               
                   
               
               
                  11 
                 AK 
                 AK093878.1 
                  1554- 
                 AGAATTTCTTGGTAGCTTTACAC 
               
               
                   
                 093878 
                   
                  1653 
                 CGAAAAATGCGTGTAACTAAAT 
               
               
                   
                   
                   
                   
                 ACCAGACATCTTGACCATTCAGC 
               
               
                   
                   
                   
                   
                 TAGAACCCTGGCAGCAACAGAG 
               
               
                   
                   
                   
                   
                 CTATTTAATT 
               
               
                   
               
               
                  12 
                 AK 
                 AK094576.1 
                  1765- 
                 CCCCTCCAGCCAGCCCTGCGTGG 
               
               
                   
                 094576 
                   
                  1864 
                 TTGTGGCCCCACTGCAGAAACGC 
               
               
                   
                   
                   
                   
                 CTCCGCTTAACACTCCAGCCTCT 
               
               
                   
                   
                   
                   
                 CTTCTATTCGGTCAGGCCACAGC 
               
               
                   
                   
                   
                   
                 TGCTGACT 
               
               
                   
               
               
                  13 
                 AK 
                 AK124143.1 
                  2252- 
                 GTACCTGGTAGAAATTGTGTCTT 
               
               
                   
                 124143 
                   
                  2351 
                 GGAATGACCCTTTCGAGTTATTG 
               
               
                   
                   
                   
                   
                 ACATGGCTCTGATGAATAGAACA 
               
               
                   
                   
                   
                   
                 TGAGCCCCAAAACTAAATCCAA 
               
               
                   
                   
                   
                   
                 AAGGAATTT 
               
               
                   
               
               
                  14 
                 AK 
                 AK126342.1 
                  2906- 
                 CTTATTGATTAGTGAATGTAGCT 
               
               
                   
                 126342 
                   
                  3005 
                 TAAGCCTTTGTATGTGTCCTCAG 
               
               
                   
                   
                   
                   
                 GGGGCAGACCGACTTTAAGAGG 
               
               
                   
                   
                   
                   
                 GACCAGATAACGTTTGAATGGA 
               
               
                   
                   
                   
                   
                 GGGATTATAT 
               
               
                   
               
               
                  15 
                 AKAP4 
                 NM_139289.1 
                   417- 
                 CTGTAAGTGTCCTCAACTGGCTT 
               
               
                   
                   
                   
                   516 
                 CTCAGTGATCTCCAGAAGTATGC 
               
               
                   
                   
                   
                   
                 CTTGGGTTTCCAACATGCACTGA 
               
               
                   
                   
                   
                   
                 GCCCCTCAACCTCTACCTGTAAA 
               
               
                   
                   
                   
                   
                 CATAAAGT 
               
               
                   
               
               
                  16 
                 AKR1C3 
                 NM_003739.4 
                  1097- 
                 GAGGACGTCTCTATGCCGGTGAC 
               
               
                   
                   
                   
                  1196 
                 TGGACATATCACCTCTACTTAAA 
               
               
                   
                   
                   
                   
                 TCCGTCCTGTTTAGCGACTTCAG 
               
               
                   
                   
                   
                   
                 TCAACTACAGCTGAGTCCATAGG 
               
               
                   
                   
                   
                   
                 CCAGAAAG 
               
               
                   
               
               
                  17 
                 ALAS1 
                 NM_000688.4 
                  1616- 
                 GGGGATCGGGATGGAGTCATGC 
               
               
                   
                   
                   
                  1715 
                 CAAAAATGGACATCATTTCTGGA 
               
               
                   
                   
                   
                   
                 ACACTTGGCAAAGCCTTTGGTTG 
               
               
                   
                   
                   
                   
                 TGTTGGAGGGTACATCGCCAGCA 
               
               
                   
                   
                   
                   
                 CGAGTTCTC 
               
               
                   
               
               
                  18 
                 AMD1 
                 NM_001634.4 
                   572- 
                 ACCACCCTCTTGCTGAAAGCACT 
               
               
                   
                   
                   
                   671 
                 GGTTCCCCTGTTGAAGCTTGCTA 
               
               
                   
                   
                   
                   
                 GGGATTACAGTGGGTTTGACTCA 
               
               
                   
                   
                   
                   
                 ATTCAAAGCTTCTTTTATTCTCG 
               
               
                   
                   
                   
                   
                 TAAGAATT 
               
               
                   
               
               
                  19 
                 AMPD3 
                 NM_000480.2 
                  3389- 
                 GTGATGCTCAGGGGCTGTCAAAG 
               
               
                   
                   
                   
                  3488 
                 TGACTGCGTTCATCAGTTTTACA 
               
               
                   
                   
                   
                   
                 CTGGGGCTGCTACATAATATTTT 
               
               
                   
                   
                   
                   
                 CATTTGAACGAAGAACTTCAAAA 
               
               
                   
                   
                   
                   
                 AGCACAGG 
               
               
                   
               
               
                  20 
                 ANKHD1 
                 NM_017747.2 
                  7665- 
                 CTTGGAACCCTATGATAAAAGTT 
               
               
                   
                   
                   
                  7764 
                 ATCCAAAATTCAACTGAATGCAC 
               
               
                   
                   
                   
                   
                 TGATGCCCAGCAGATTTGGCCTG 
               
               
                   
                   
                   
                   
                 GCACGTGGGCACCTCATATTGGA 
               
               
                   
                   
                   
                   
                 AACATGCA 
               
               
                   
               
               
                  21 
                 ANP32B 
                 NM_006401.2 
                   661- 
                 CACCTTGGAACCTTTGAAAAAGT 
               
               
                   
                   
                   
                   760 
                 TAGAATGTCTGAAAAGCCTGGAC 
               
               
                   
                   
                   
                   
                 CTCTTTAACTGTGAGGTTACCAA 
               
               
                   
                   
                   
                   
                 CCTGAATGACTACCGAGAGAGT 
               
               
                   
                   
                   
                   
                 GTCTTCAAG 
               
               
                   
               
               
                  22 
                 ANXA1  
                 NM_000700.1 
                   516- 
                 GAAATCAGAGACATTAACAGGG 
               
               
                   
                 b 
                   
                   615 
                 TCTACAGAGAGGAACTGAAGAG 
               
               
                   
                   
                   
                   
                 AGATCTGGCCAAAGACATAACCT 
               
               
                   
                   
                   
                   
                 CAGACACATCTGGAGATTTTCGG 
               
               
                   
                   
                   
                   
                 AACGCTTTGC 
               
               
                   
               
               
                  23 
                 ANXA1 
                 NM_000700.2 
                  1191- 
                 TGGATGAAACCAAAGGAGATTA 
               
               
                   
                   
                   
                  1290 
                 TGAGAAAATCCTGGTGGCTCTTT 
               
               
                   
                   
                   
                   
                 GTGGAGGAAACTAAACATTCCCT 
               
               
                   
                   
                   
                   
                 TGATGGTCTCAAGCTATGATCAG 
               
               
                   
                   
                   
                   
                 AAGACTTTA 
               
               
                   
               
               
                  24 
                 AP2S1 
                 NM_021575.3 
                   746- 
                 CGAGTAACCGTGCCGTTGTCGTG 
               
               
                   
                   
                   
                   845 
                 TGATGCCATAAGCGTCTGTGCGT 
               
               
                   
                   
                   
                   
                 GGAGTCCCCAATAAACCTGTGGT 
               
               
                   
                   
                   
                   
                 CCTGCCTGGCCTTGCCGTCAAAA 
               
               
                   
                   
                   
                   
                 AAAAAAAA 
               
               
                   
               
               
                  25 
                 CENTD2 
                 NM_00104011 
                  4923- 
                 AAACTCCAGAACAGCAGAAAGC 
               
               
                   
                   
                 8.2 
                  5022 
                 GGGTGCTGTAGAGGAGCACTCA 
               
               
                   
                   
                   
                   
                 GCTCACGGGGAGGGAGCTCTTG 
               
               
                   
                   
                   
                   
                 GCTGAGCTTCTACAGGGCTGAGA 
               
               
                   
                   
                   
                   
                 GCTGCGCTTTG 
               
               
                   
               
               
                  26 
                 ARCN1 
                 NM_001655.4 
                  3437- 
                 CACTTTTAGCTGGTTGAAAAGTA 
               
               
                   
                   
                   
                  3536 
                 CCACTCCCACTCTGAACATCTGG 
               
               
                   
                   
                   
                   
                 CCGTCCCTGCAAAGAGTGTACTG 
               
               
                   
                   
                   
                   
                 TGCTTGAAGCAGAGCACTCACAC 
               
               
                   
                   
                   
                   
                 ATAAATGG 
               
               
                   
               
               
                  27 
                 ARG1  
                 NM_000045.2 
                   506- 
                 AAGGAACTAAAAGGAAAGATTC 
               
               
                   
                 b 
                   
                   605 
                 CCGATGTGCCAGGATTCTCCTGG 
               
               
                   
                   
                   
                   
                 GTGACTCCCTGTATATCTGCCAA 
               
               
                   
                   
                   
                   
                 GGATATTGTGTATATTGGCTTGA 
               
               
                   
                   
                   
                   
                 GAGACGTGG 
               
               
                   
               
               
                  28 
                 ARG1 
                 NM_000045.3 
                   989- 
                 TTCGGACTTGCTCGGGAGGGTAA 
               
               
                   
                   
                   
                  1088 
                 TCACAAGCCTATTGACTACCTTA 
               
               
                   
                   
                   
                   
                 ACCCACCTAAGTAAATGTGGAA 
               
               
                   
                   
                   
                   
                 ACATCCGATATAAATCTCATAGT 
               
               
                   
                   
                   
                   
                 TAATGGCAT 
               
               
                   
               
               
                  29 
                 ARHGAP 
                 NM_018054.5 
                  3027- 
                 CATGTATGGTCTGTGTCTCCCCA 
               
               
                   
                 17 
                   
                  3126 
                 GTCCCCTCAGAACCATGCCCATG 
               
               
                   
                   
                   
                   
                 GATGGTGACTGCTGGCTCTGTCA 
               
               
                   
                   
                   
                   
                 CCTCATCAAACTGGATGTGACCC 
               
               
                   
                   
                   
                   
                 ATGCCGCC 
               
               
                   
               
               
                  30 
                 ARHGAP 
                 NM_033515.2 
                  2499- 
                 TTTTTGACCAAAAAGATAACAAA 
               
               
                   
                 18 
                   
                  2598 
                 TACCAGGTATGGCAAGTTGTGAA 
               
               
                   
                   
                   
                   
                 GACAGCACATTAAAACATACCTA 
               
               
                   
                   
                   
                   
                 ATTTCACAGTATTCCTGTCACGA 
               
               
                   
                   
                   
                   
                 CAGAATGT 
               
               
                   
               
               
                  31 
                 ARHGAP 
                 NM_015071.4 
                  6088- 
                 TCCCTGAGCTTTCCCAGTAGCCT 
               
               
                   
                 26 
                   
                  6187 
                 CCAGTTTCCTTTGTAAGACCCAG 
               
               
                   
                   
                   
                   
                 GGATCACTTAGCCATAGCCTGAA 
               
               
                   
                   
                   
                   
                 TCTTTTAGGGGTATTAAGGTCAG 
               
               
                   
                   
                   
                   
                 CCTCTCAC 
               
               
                   
               
               
                  32 
                 ARHGEF 
                 NM_015318.3 
                  5128- 
                 GATTACAACATTTCCTCACTGCG 
               
               
                   
                 18 
                   
                  5227 
                 GGATATTTCTGACCCGCTTTAGA 
               
               
                   
                   
                   
                   
                 ACTTAAGACCTGATTCTAGCAAT 
               
               
                   
                   
                   
                   
                 AAACGTGTCCGAGATGAGCGGT 
               
               
                   
                   
                   
                   
                 GAAAAAAAA 
               
               
                   
               
               
                  33 
                 FLJ 
                 NM_018071.4 
                  5402- 
                 GAATGTGTCTCCTCCACAGTGGC 
               
               
                   
                 10357 
                   
                  5501 
                 TCCCAGAGGTTCCACACACTCTC 
               
               
                   
                   
                   
                   
                 TGAAGCTCCTTCTCCCACACTGC 
               
               
                   
                   
                   
                   
                 ACCTACTCCTTGAGGCTGAACTG 
               
               
                   
                   
                   
                   
                 GTCACAGA 
               
               
                   
               
               
                  34 
                 ARHGEF 
                 NM_005435.3 
                  5151- 
                 GGGGGACCATTGGGGCCTGAGC 
               
               
                   
                 5 
                   
                  5250 
                 CAAGGAACTTTCCTTCTACTGCC 
               
               
                   
                   
                   
                   
                 TTATAGTGCTTAAACATTCTCCG 
               
               
                   
                   
                   
                   
                 CCTCCAGGGTGCAGATTCAGAGC 
               
               
                   
                   
                   
                   
                 TGGCCAGAG 
               
               
                   
               
               
                  35 
                 ARL8B 
                 NM_018184.2 
                  2491- 
                 ACCATTACAAAGAATGTGGCAA 
               
               
                   
                   
                   
                  2590 
                 CTTGCTTGTGCCTAAAAGGAGGA 
               
               
                   
                   
                   
                   
                 ATTGGAACTAGAATGTGTGACTC 
               
               
                   
                   
                   
                   
                 TGTGGGGACTGCATAGGTTTGTT 
               
               
                   
                   
                   
                   
                 AATTGACCT 
               
               
                   
               
               
                  36 
                 ARPC2 
                 NM_005731.2 
                   951- 
                 ACGGGGAAGACGTTTTCATCCCG 
               
               
                   
                   
                   
                  1050 
                 CTAATCTTGGGAATAAGAGGAG 
               
               
                   
                   
                   
                   
                 GAAGCGGCTGGCAACTGAAGGC 
               
               
                   
                   
                   
                   
                 TGGAACACTTGCTACTGGATAAT 
               
               
                   
                   
                   
                   
                 CGTAGCTTTT 
               
               
                   
               
               
                  37 
                 ASF1B 
                 NM_018154.2 
                  1476- 
                 CTGTCTCCGGGCCAGGGTCAGGG 
               
               
                   
                   
                   
                  1575 
                 ACCCTCTGCCTCTGGCAGCCTTA 
               
               
                   
                   
                   
                   
                 ACCTGTCCTCTGCTAGGACCAGG 
               
               
                   
                   
                   
                   
                 GTGATTTCAAGCCAGGGAAGCA 
               
               
                   
                   
                   
                   
                 ACTGGGACC 
               
               
                   
               
               
                  38 
                 ATG4B 
                 NM_178326.2 
                   106- 
                 GGACGCAGCTACTCTGACCTACG 
               
               
                   
                   
                   
                   205 
                 ACACTCTCCGGTTTGCTGAGTTT 
               
               
                   
                   
                   
                   
                 GAAGATTTTCCTGAGACCTCAGA 
               
               
                   
                   
                   
                   
                 GCCCGTTTGGATACTGGGTAGAA 
               
               
                   
                   
                   
                   
                 AATACAGC 
               
               
                   
               
               
                  39 
                 ATG5 
                 NM_004849.2 
                  1105- 
                 TGCAGTGGCTGAGTGAACATCTG 
               
               
                   
                   
                   
                  1204 
                 AGCTACCCGGATAATTTTCTTCA 
               
               
                   
                   
                   
                   
                 TATTAGTATCATCCCACAGCCAA 
               
               
                   
                   
                   
                   
                 CAGATTGAAGGATCAACTATTTG 
               
               
                   
                   
                   
                   
                 CCTGAACA 
               
               
                   
               
               
                  40 
                 ATM 
                 NM_000051.3 
                    31- 
                 ACGCTAAGTCGCTGGCCATTGGT 
               
               
                   
                   
                   
                   130 
                 GGACATGGCGCAGGCGCGTTTGC 
               
               
                   
                   
                   
                   
                 TCCGACGGGCCGAATGTTTTGGG 
               
               
                   
                   
                   
                   
                 GCAGTGTTTTGAGCGCGGAGACC 
               
               
                   
                   
                   
                   
                 GCGTGATA 
               
               
                   
               
               
                  41 
                 ATP2C1 
                 NM_014382.3 
                  4070- 
                 TAAAAAGTCCCCAAACCCAAAC 
               
               
                   
                   
                   
                  4169 
                 AAATGGTTTATGAACCAGAGTAT 
               
               
                   
                   
                   
                   
                 ATGTGGAAGATTCTTTGCTGGTC 
               
               
                   
                   
                   
                   
                 TTGCTCTGTGTGCATCTGAAGCT 
               
               
                   
                   
                   
                   
                 TCTTTGGCC 
               
               
                   
               
               
                  42 
                 ATP5B 
                 NM_001686.3 
                  1626- 
                 CTATATGGTGGGACCCATTGAAG 
               
               
                   
                   
                   
                  1725 
                 AAGCTGTGGCAAAAGCTGATAA 
               
               
                   
                   
                   
                   
                 GCTGGCTGAAGAGCATTCATCGT 
               
               
                   
                   
                   
                   
                 GAGGGGTCTTTGTCCTCTGTACT 
               
               
                   
                   
                   
                   
                 GTCTCTCTC 
               
               
                   
               
               
                  43 
                 ATP5I 
                 NM_007100.2 
                   256- 
                 TTGCCAGAGAATTGGCAGAAGA 
               
               
                   
                   
                   
                   355 
                 TGACAGCATATTAAAGTGAGTGA 
               
               
                   
                   
                   
                   
                 CCCTGCGACCCACTCTTTGGACC 
               
               
                   
                   
                   
                   
                 AGCAGCGGATGAATAAAGCTTC 
               
               
                   
                   
                   
                   
                 CTGTGTTGTG 
               
               
                   
               
               
                  44 
                 ATP5J2 
                 NM_004889.3 
                   267- 
                 GCTGGCATGCTACGTGCTCTTTA 
               
               
                   
                   
                   
                   366 
                 GCTACTCCTTTTCCTACAAGCAT 
               
               
                   
                   
                   
                   
                 CTCAAGCACGAGCGGCTCCGCA 
               
               
                   
                   
                   
                   
                 AATACCACTGAAGAGGACACAC 
               
               
                   
                   
                   
                   
                 TCTGCACCCC 
               
               
                   
               
               
                  45 
                 ATP5L 
                 NM_006476.4 
                   196- 
                 GGGACGGGGTCCTGCAGCGGGT 
               
               
                   
                   
                   
                   295 
                 CCTTCCGGCGGGTGACATTCAGC 
               
               
                   
                   
                   
                   
                 CGGCGGTTCGGGGCGACGGACT 
               
               
                   
                   
                   
                   
                 CTCCATTCCAGAACCATGGCCCA 
               
               
                   
                   
                   
                   
                 ATTTGTCCGT 
               
               
                   
               
               
                  46 
                 AW 
                 AW173314.1 
                   419- 
                 AGCAGAAGGCAGGGGAGTCCAC 
               
               
                   
                 173314 
                   
                   518 
                 ACAGGGCAAGCAGCAACCAGGC 
               
               
                   
                   
                   
                   
                 TTCTGAGGACAGGAAAGGAGGG 
               
               
                   
                   
                   
                   
                 AGCATCTGGTGGGAAGCTGGCG 
               
               
                   
                   
                   
                   
                 AGGAGGGGCTGG 
               
               
                   
               
               
                  47 
                 AW 
                 AW270402.1 
                   203- 
                 GATATCTCACACACGGAATAATC 
               
               
                   
                 270402 
                   
                   302 
                 ATTAAGAAACAACCACTGTTGAG 
               
               
                   
                   
                   
                   
                 CAAAGTTGATAGGCAGTAAGGA 
               
               
                   
                   
                   
                   
                 AATAAAGTGGACATAAACACAG 
               
               
                   
                   
                   
                   
                 CAGTACTAAT 
               
               
                   
               
               
                  48 
                 AZI2 
                 NM_022461.4 
                  3031- 
                 GAATTGGTGTCAGATGCTGGAAT 
               
               
                   
                   
                   
                  3130 
                 TTATTCTGACCAATGAACACAGC 
               
               
                   
                   
                   
                   
                 TGACTCAGGGGAGTACAATCTCC 
               
               
                   
                   
                   
                   
                 TGCCAAGTAATAGAACCAAACC 
               
               
                   
                   
                   
                   
                 CAATATGCA 
               
               
                   
               
               
                  49 
                 BACH2 
                 NM_021813.3 
                  8696- 
                 TCCAGAACCAGTCTGATGCAAGT 
               
               
                   
                   
                   
                  8795 
                 GCACCTCTAATATATGCCTTACA 
               
               
                   
                   
                   
                   
                 AACTCCAGAGGCCATATTCAAAA 
               
               
                   
                   
                   
                   
                 CAGGGTCTTCTCAGTGTATGCAA 
               
               
                   
                   
                   
                   
                 GGGGCTGC 
               
               
                   
               
               
                  50 
                 BAG3 
                 NM_004281.3 
                  2304- 
                 CCCCACCACCTGTTAGCTGTGGT 
               
               
                   
                   
                   
                  2403 
                 TGTGCACTGTCTTTTGTAGCTCT 
               
               
                   
                   
                   
                   
                 GGACTGGAGGGGTAGATGGGGAG 
               
               
                   
                   
                   
                   
                 TCAATTACCCATCACATAAATAT 
               
               
                   
                   
                   
                   
                 GAAACATT 
               
               
                   
               
               
                  51 
                 BANP 
                 NM_079837.2 
                  2125- 
                 GGAGCCCTTTGCTGTGTGCTCTG 
               
               
                   
                   
                   
                  2224 
                 TCCAGTGTCATGAGGCAGGTGTT 
               
               
                   
                   
                   
                   
                 TGCAAAGCCAGCTCTCGGTTCCG 
               
               
                   
                   
                   
                   
                 ATGGGGTATTGCTGACCTACTTT 
               
               
                   
                   
                   
                   
                 TCTAGGGG 
               
               
                   
               
               
                  52 
                 BATF 
                 NM_006399.3 
                   294- 
                 CCTGGCAAACAGGACTCATCTGA 
               
               
                   
                   
                   
                   393 
                 TGATGTGAGAAGAGTTCAGAGG 
               
               
                   
                   
                   
                   
                 AGGGAGAAAAATCGTATTGCCG 
               
               
                   
                   
                   
                   
                 CCCAGAAGAGCCGACAGAGGCA 
               
               
                   
                   
                   
                   
                 GACACAGAAGG 
               
               
                   
               
               
                  53 
                 BCL10 
                 NM_003921.2 
                  1251- 
                 TGAAAATACCATCTTCTCTTCAA 
               
               
                   
                   
                   
                  1350 
                 CTACACTTCCCAGACCTGGGGAC 
               
               
                   
                   
                   
                   
                 CCAGGGGCTCCTCCTTTGCCACC 
               
               
                   
                   
                   
                   
                 AGATCTACAGTTAGAAGAAGAA 
               
               
                   
                   
                   
                   
                 GGAACTTGT 
               
               
                   
               
               
                  54 
                 BCL6 
                 NM_00113084 
                  3401- 
                 CCTCACGGTGCCTTTTTTCACGG 
               
               
                   
                   
                 5.1 
                  3500 
                 AAGTTTTCAATGATGGGCGAGCG 
               
               
                   
                   
                   
                   
                 TGCACCATCCCTTTTTGAAGTGT 
               
               
                   
                   
                   
                   
                 AGGCAGACACAGGGACTTGAAG 
               
               
                   
                   
                   
                   
                 TTGTTACTA 
               
               
                   
               
               
                  55 
                 BCOR 
                 NM_017745.5 
                  5794- 
                 ATACAAAGCTCTGATGACAGGCC 
               
               
                   
                   
                   
                  5893 
                 ATGACTGTAGAGTGGTCAGAACT 
               
               
                   
                   
                   
                   
                 GTGTGGTTGGTTTGAGGGAGCGA 
               
               
                   
                   
                   
                   
                 ATTCGGGGAAGGCACTTGGTGAT 
               
               
                   
                   
                   
                   
                 ATAACTTT 
               
               
                   
               
               
                  56 
                 BF 
                 BF375676.1 
                   141- 
                 TGTATTTCTGTGCAATGAGAGAG 
               
               
                   
                 375676 
                   
                   240 
                 GCTCTTTATGGTGGTGCTACAAA 
               
               
                   
                   
                   
                   
                 CAAGCTCATCTTTGGAACTGGCA 
               
               
                   
                   
                   
                   
                 CTCTGCTTGCTGTCCAGCCAAAT 
               
               
                   
                   
                   
                   
                 ATCCAGAA 
               
               
                   
               
               
                  57 
                 BID 
                 NM_001196.2 
                  1876- 
                 AAGCACGACAGTGGATGCTGGG 
               
               
                   
                   
                   
                  1975 
                 TCCATATCACACACATTGCTGTG 
               
               
                   
                   
                   
                   
                 AACAGGAAACTCCTGTGACCAC 
               
               
                   
                   
                   
                   
                 AACATGAGGCCACTGGAGACGC 
               
               
                   
                   
                   
                   
                 ATATGAGTAAG 
               
               
                   
               
               
                  58 
                 BMPR2 
                 NM_001204.6 
                  1164- 
                 CAGCGGCCCTGGCGGGTGCCCTG 
               
               
                   
                   
                   
                  1263 
                 GCTACCATGGACCATCCTGCTGG 
               
               
                   
                   
                   
                   
                 TCAGCACTGCGGCTGCTTCGCAG 
               
               
                   
                   
                   
                   
                 AATCAAGAACGGCTATGTGCGTT 
               
               
                   
                   
                   
                   
                 TAAAGATC 
               
               
                   
               
               
                  59 
                 BQ 
                 BQ189294.1 
                   416- 
                 GCTGGAGTGATTGGCCCTGATGA 
               
               
                   
                 189294 
                   
                   515 
                 CCATGGAGAAAAGAGAGTAGGG 
               
               
                   
                   
                   
                   
                 AGAACAGTATAACCAGAAGTCA 
               
               
                   
                   
                   
                   
                 GGGGGGTCTCCTGGAATCCCTCC 
               
               
                   
                   
                   
                   
                 TCACAATACC 
               
               
                   
               
               
                  60 
                 BU 
                 BU743228.1 
                   154- 
                 CCCTGTGGGCCTTGCAGGCCAGT 
               
               
                   
                 743228 
                   
                   253 
                 CCAGGCAGGTCTTTCACACTGTT 
               
               
                   
                   
                   
                   
                 GTCCCACATAACAGAAAAAGCT 
               
               
                   
                   
                   
                   
                 GAGCAGACAGGGTAGGAAACAC 
               
               
                   
                   
                   
                   
                 ACTTGCATCT 
               
               
                   
               
               
                  61 
                 BX 
                 BX089765.1 
                   106- 
                 TTAAGCAACTTGCTCCAGTGACG 
               
               
                   
                 089765 
                   
                   205 
                 CAGCTGGTAAGCAGCAGAGCTG 
               
               
                   
                   
                   
                   
                 GGATTAAAACCCAGGCATTCTGA 
               
               
                   
                   
                   
                   
                 TTCCACCACCTACACACTTAGCC 
               
               
                   
                   
                   
                   
                 ATTCCGCCC 
               
               
                   
               
               
                  62 
                 BX 
                 BX108566.1 
                   365- 
                 ATTTAGGGTGAGAGCTTCACAGC 
               
               
                   
                 108566 
                   
                   464 
                 TGAAAATCTCCTTTAAAGAAAAC 
               
               
                   
                   
                   
                   
                 GCGGCCCAAATGTGCTGGGAGG 
               
               
                   
                   
                   
                   
                 AGAAGCCAGTGGATCTAGGAGG 
               
               
                   
                   
                   
                   
                 GGGCCCGGCG 
               
               
                   
               
               
                  63 
                 BX 
                 BX400436.2 
                     1- 
                 ATATTTTGGAGAGGGAAGTTGGC 
               
               
                   
                 400436 
                   
                   100 
                 TCACTGTTGTAGAGGACCTGAAC 
               
               
                   
                   
                   
                   
                 AAGGTGTTCCCACCCGAGGTCGC 
               
               
                   
                   
                   
                   
                 TGTGTTTGAGCCATCAGAAGCAG 
               
               
                   
                   
                   
                   
                 AGATCTCC 
               
               
                   
               
               
                  64 
                 BX 
                 BX436458.2 
                   518- 
                 ATGCAGACAATTTGCCTGTGAGA 
               
               
                   
                 436458 
                   
                   617 
                 TGAGGAAAATTCTCTGGAAGATT 
               
               
                   
                   
                   
                   
                 TAGGCCCTGAGAGCTGAAAAGG 
               
               
                   
                   
                   
                   
                 GACCCTAAACATTACCTGGTGAC 
               
               
                   
                   
                   
                   
                 AACTGCCCT 
               
               
                   
               
               
                  65 
                 C15orf 
                 NM_015492.4 
                  3535- 
                 CCTGAGCTTTTAACGTGAGGGTC 
               
               
                   
                 39 
                   
                  3634 
                 TTTATTGGATAGGACTACTCCCT 
               
               
                   
                   
                   
                   
                 ATTTCTTGCCTAGAGAACACACA 
               
               
                   
                   
                   
                   
                 TGGGCTTTGGAGCCCGACAGACC 
               
               
                   
                   
                   
                   
                 TGGGCTTG 
               
               
                   
               
               
                  66 
                 C17orf 
                 XM_944416.1 
                  4909- 
                 AAGGATGGGGGTGGATTGACCA 
               
               
                   
                 51 
                   
                  5008 
                 AGCTGGGCCAGAGGTGCGAGGA 
               
               
                   
                   
                   
                   
                 GCTGATCTGCGAGCCCTGTGTGC 
               
               
                   
                   
                   
                   
                 CTGTGAGTCCTGGCGGAGTGGCC 
               
               
                   
                   
                   
                   
                 GTGCGTGGTG 
               
               
                   
               
               
                  67 
                 C3 
                 NM_000064.2 
                  4397- 
                 CATCTACCTGGACAAGGTCTCAC 
               
               
                   
                   
                   
                  4496 
                 ACTCTGAGGATGACTGTCTAGCT 
               
               
                   
                   
                   
                   
                 TTCAAAGTTCACCAATACTTTAA 
               
               
                   
                   
                   
                   
                 TGTAGAGCTTATCCAGCCTGGAG 
               
               
                   
                   
                   
                   
                 CAGTCAAG 
               
               
                   
               
               
                  68 
                 C4B 
                 NM_00100202 
                  4438- 
                 GAGTCCAGGGTGCACTACACCGT 
               
               
                   
                   
                 9.3 
                  4537 
                 GTGCATCTGGCGGAACGGCAAG 
               
               
                   
                   
                   
                   
                 GTGGGGCTGTCTGGCATGGCCAT 
               
               
                   
                   
                   
                   
                 CGCGGACGTCACCCTCCTGAGTG 
               
               
                   
                   
                   
                   
                 GATTCCACG 
               
               
                   
               
               
                  69 
                 C4orf 
                 NM_017867.2 
                   682- 
                 GAACCGTGAAGATGAAACAGAG 
               
               
                   
                 27 
                   
                   781 
                 AGATAAGAAAGTTGTGACAAAG 
               
               
                   
                   
                   
                   
                 ACCTTTCATGGTGCAGGCTTGGT 
               
               
                   
                   
                   
                   
                 TGTTCCAGTAGATAAAAATGATG 
               
               
                   
                   
                   
                   
                 TTGGGTACCG 
               
               
                   
               
               
                  70 
                 C8orf 
                 NM_032847.2 
                  1029- 
                 TAAAAGATGAAGTTCACCCAGA 
               
               
                   
                 76 
                   
                  1128 
                 GGTGAAGTGTGTTGGCTCCGTAG 
               
               
                   
                   
                   
                   
                 CCCTGACTGCCTTGGTGACTGTA 
               
               
                   
                   
                   
                   
                 TCCTCAGAAGAATTTGAAGACAA 
               
               
                   
                   
                   
                   
                 GTGGTTCAG 
               
               
                   
               
               
                  71 
                 C9orf 
                 NM_182635.1 
                   529- 
                 CGCTGGCCATGGGGAAGCCACCT 
               
               
                   
                 164 
                   
                   628 
                 CCAGGGCAGTCCCAGGGACTGA 
               
               
                   
                   
                   
                   
                 ATTGGAAGTTGTCCCAAGTCACT 
               
               
                   
                   
                   
                   
                 TCAGGTCCAACTGGGACAGCAG 
               
               
                   
                   
                   
                   
                 AGGTAACCCC 
               
               
                   
               
               
                  72 
                 CAMP 
                 NM_004345.4 
                   623- 
                 TTGTCCAGAGAATCAAGGATTTT 
               
               
                   
                   
                   
                   722 
                 TTGCGGAATCTTGTACCCAGGAC 
               
               
                   
                   
                   
                   
                 AGAGTCCTAGTGTGTGCCCTACC 
               
               
                   
                   
                   
                   
                 CTGGCTCAGGCTTCTGGGCTCTG 
               
               
                   
                   
                   
                   
                 AGAAATAA 
               
               
                   
               
               
                  73 
                 CASP1 
                 NM_033294.3 
                   219- 
                 ATTTATCCAATAATGGACAAGTC 
               
               
                   
                   
                   
                   318 
                 AAGCCGCACACGTCTTGCTCTCA 
               
               
                   
                   
                   
                   
                 TTATCTGCAATGAAGAATTTGAC 
               
               
                   
                   
                   
                   
                 AGTATTCCTAGAAGAACTGGAGC 
               
               
                   
                   
                   
                   
                 TGAGGTTG 
               
               
                   
               
               
                  74 
                 CASP2 
                 NM_032983.3 
                  3347- 
                 CCCACCACTCTTGACTCAGGTGG 
               
               
                   
                   
                   
                  3446 
                 TGTCCTTCTTCCTCAAGTCTTGA 
               
               
                   
                   
                   
                   
                 CAATTCCCGGGCCCTTCAGTCCC 
               
               
                   
                   
                   
                   
                 TGAGCAGTCTACTTCTGTGTCT 
               
               
                   
                   
                   
                   
                 GTCACCACA 
               
               
                   
               
               
                  75 
                 CASP3 
                 NM_032991.2 
                   686- 
                 ACTCCACAGCACCTGGTTATTAT 
               
               
                   
                   
                   
                   785 
                 TCTTGGCGAAATTCAAAGGATGG 
               
               
                   
                   
                   
                   
                 CTCCTGGTTCATCCAGTCGCTTT 
               
               
                   
                   
                   
                   
                 GTGCCATGCTGAAACAGTATGCC 
               
               
                   
                   
                   
                   
                 GACAAGCT 
               
               
                   
               
               
                  76 
                 CBLL1 
                 NM_024814.3 
                  1967- 
                 ATGAGGGGGAAAAAAACTTATG 
               
               
                   
                   
                   
                  2066 
                 TGTAGTCAATCTTTTAAGCTTTG 
               
               
                   
                   
                   
                   
                 ACTGTTTTGGGAAGGAAGAGTAC 
               
               
                   
                   
                   
                   
                 CTCTTATCGAGGTAGTATAAAAC 
               
               
                   
                   
                   
                   
                 ACATAGGGT 
               
               
                   
               
               
                  77 
                 CC2D1B 
                 NM_032449.2 
                  4183- 
                 TTGCATAAGCACAGCTCAAGAAC 
               
               
                   
                   
                   
                  4282 
                 TGAGCTTTGTATGTGTCCTTTTG 
               
               
                   
                   
                   
                   
                 GGGGATAACAGGGCTGGACCATG 
               
               
                   
                   
                   
                   
                 CTTCCCTGCCCTTAAACGCAGAG 
               
               
                   
                   
                   
                   
                 CTTTTAGT 
               
               
                   
               
               
                  78 
                 KIAA 
                 NM_021174.5 
                   201- 
                 GGGAGAGGGCCCACACAGTCTC 
               
               
                   
                 1967 
                   
                   300 
                 CTCGCCGGCACCGGCCTCCTCCA 
               
               
                   
                   
                   
                   
                 TTTTTCCGGGCCTTGCGTGGAGG 
               
               
                   
                   
                   
                   
                 GTTTTGGCGGATGTTTTTGAACG 
               
               
                   
                   
                   
                   
                 AAGGAATGT 
               
               
                   
               
               
                  79 
                 CCDC97 
                 NM_052848.1 
                  2867- 
                 ATCCAGAGTGAGACAGCATTGG 
               
               
                   
                   
                   
                  2966 
                 AGGGACAAGTGTGCATGCAGAT 
               
               
                   
                   
                   
                   
                 GTCCTCAGACGGGAAGGTTTGAG 
               
               
                   
                   
                   
                   
                 AAGGGTCAGATGGTAGGCGGGC 
               
               
                   
                   
                   
                   
                 CTAACAAGGGC 
               
               
                   
               
               
                  80 
                 CCL3 
                 NM_002983.2 
                   160- 
                 CAGTTCTCTGCATCACTTGCTGC 
               
               
                   
                   
                   
                   259 
                 TGACACGCCGACCGCCTGCTGCT 
               
               
                   
                   
                   
                   
                 TCAGCTACACCTCCCGGCAGATT 
               
               
                   
                   
                   
                   
                 CCACAGAATTTCATAGCTGACTA 
               
               
                   
                   
                   
                   
                 CTTTGAGA 
               
               
                   
               
               
                  81 
                 CCL3L1 
                 NM_021006.4 
                   422- 
                 GGAGCCTGAGCCTTGGGAACAT 
               
               
                   
                   
                   
                   521 
                 GCGTGTGACCTCTACAGCTACCT 
               
               
                   
                   
                   
                   
                 CTTCTATGGACTGGTTATTGCCA 
               
               
                   
                   
                   
                   
                 AACAGCCACACTGTGGGACTCTT 
               
               
                   
                   
                   
                   
                 CTTAACTTA 
               
               
                   
               
               
                  82 
                 CCL3L3 
                 NM_00100143 
                   402- 
                 GGGGAGGAGCAGGAGCCTGAGC 
               
               
                   
                   
                 7.3 
                   501 
                 CTTGGGAACATGCGTGTGACCTC 
               
               
                   
                   
                   
                   
                 CACAGCTACCTCTTCTATGGACT 
               
               
                   
                   
                   
                   
                 GGTTATTGCCAAACAGCCACACT 
               
               
                   
                   
                   
                   
                 GTGGGACTC 
               
               
                   
               
               
                  83 
                 CCL4 
                 NM_002984.2 
                    36- 
                 TTCTGCAGCCTCACCTCTGAGAA 
               
               
                   
                   
                   
                   135 
                 AACCTCTTTGCCACCAATACCAT 
               
               
                   
                   
                   
                   
                 GAAGCTCTGCGTGACTGTCCTGT 
               
               
                   
                   
                   
                   
                 CTCTCCTCATGCTAGTAGCTGCC 
               
               
                   
                   
                   
                   
                 TTCTGCTC 
               
               
                   
               
               
                  84 
                 CCND3 
                 NM_001760.2 
                  1216- 
                 GGCCAGCCATGTCTGCATTTCGG 
               
               
                   
                   
                   
                  1315 
                 TGGCTAGTCAAGCTCCTCCTCCC 
               
               
                   
                   
                   
                   
                 TGCATCTGACCAGCAGCGCCTTT 
               
               
                   
                   
                   
                   
                 CCCAACTCTAGCTGGGGGTGGGC 
               
               
                   
                   
                   
                   
                 CAGGCTGA 
               
               
                   
               
               
                  85 
                 CCR1 
                 NM_001295.2 
                   536- 
                 CATCATTTGGGCCCTGGCCATCT 
               
               
                   
                   
                   
                   635 
                 TGGCTTCCATGCCAGGCTTATAC 
               
               
                   
                   
                   
                   
                 TTTTCCAAGACCCAATGGGAATT 
               
               
                   
                   
                   
                   
                 CACTCACCACACCTGCAGCCTTC 
               
               
                   
                   
                   
                   
                 ACTTTCCT 
               
               
                   
               
               
                  86 
                 CCR6 
                 NM_031409.2 
                   936- 
                 CTTTAACTGCGGGATGCTGCTCC 
               
               
                   
                   
                   
                  1035 
                 TGACTTGCATTAGCATGGACCGG 
               
               
                   
                   
                   
                   
                 TACATCGCCATTGTACAGGCGAC 
               
               
                   
                   
                   
                   
                 TAAGTCATTCCGGCTCCGATCCA 
               
               
                   
                   
                   
                   
                 GAACACTA 
               
               
                   
               
               
                  87 
                 CCR9 
                 NM_031200.1 
                  1096- 
                 CCCTGTTCTCTATGTTTTTGTGG 
               
               
                   
                   
                   
                  1195 
                 GTGAGAGATTCCGCCGGGATCTC 
               
               
                   
                   
                   
                   
                 GTGAAAACCCTGAAGAACTTGGG 
               
               
                   
                   
                   
                   
                 TTGCATCAGCCAGGCCCAGTGGG 
               
               
                   
                   
                   
                   
                 TTTCATTT 
               
               
                   
               
               
                  88 
                 CCT6A 
                 NM_001762.3 
                   281- 
                 GCCCAAGGGCACCATGAAGATG 
               
               
                   
                   
                   
                   380 
                 CTCGTTTCTGGCGCTGGAGACAT 
               
               
                   
                   
                   
                   
                 CAAACTTACTAAAGACGGCAAT 
               
               
                   
                   
                   
                   
                 GTGCTGCTTCACGAAATGCAAAT 
               
               
                   
                   
                   
                   
                 TCAACACCCA 
               
               
                   
               
               
                  89 
                 CD14 
                 NM_000591.2 
                   886- 
                 GCCCAAGCACACTCGCCTGCCTT 
               
               
                   
                   
                   
                   985 
                 TTCCTGCGAACAGGTTCGCGCCT 
               
               
                   
                   
                   
                   
                 TCCCGGCCCTTACCAGCCTAGAC 
               
               
                   
                   
                   
                   
                 CTGTCTGACAATCCTGGACTGGG 
               
               
                   
                   
                   
                   
                 CGAACGCG 
               
               
                   
               
               
                  90 
                 CD160  
                 NM_007053.2 
                   501- 
                 TTGATGTTCACCATAAGCCAAGT 
               
               
                   
                 b 
                   
                   600 
                 CACACCGTTGCACAGTGGGACCT 
               
               
                   
                   
                   
                   
                 ACCAGTGTTGTGCCAGAAGCCAG 
               
               
                   
                   
                   
                   
                 AAGTCAGGTATCCGCCTTCAGGG 
               
               
                   
                   
                   
                   
                 CCATTTTT 
               
               
                   
               
               
                  91 
                 CD160 
                 NM_007053.3 
                  1286- 
                 AAAGGAAGACAGCCAGATCCAG 
               
               
                   
                   
                   
                  1385 
                 TGATTGACTTGGCATGAAAATGA 
               
               
                   
                   
                   
                   
                 GAAAATGCAGACAGACCTCAAC 
               
               
                   
                   
                   
                   
                 ATTCAACAACATCCATACAGCAC 
               
               
                   
                   
                   
                   
                 TGCTGGAGGA 
               
               
                   
               
               
                  92 
                 CD1A 
                 NM_001763.2 
                  1816- 
                 CCTGTTTTAGATATCCCTTACTC 
               
               
                   
                   
                   
                  1915 
                 CAGAGGGCCTTCCCTGACTTACA 
               
               
                   
                   
                   
                   
                 AGTGGGAAGCAGTCTCTTCCTGG 
               
               
                   
                   
                   
                   
                 TCTGAACTCCCGCCACATTTTAG 
               
               
                   
                   
                   
                   
                 CCGTACTT 
               
               
                   
               
               
                  93 
                 CD36 
                 NM_000072.3 
                  1619- 
                 TAAAGAATCTGAAGAGGAACTA 
               
               
                   
                   
                   
                  1718 
                 TATTGTGCCTATTCTTTGGCTTA 
               
               
                   
                   
                   
                   
                 ATGAGACTGGGACCATTGGTGAT 
               
               
                   
                   
                   
                   
                 GAGAAGGCAAACATGTTCAGAAG 
               
               
                   
                   
                   
                   
                 TCAAGTAAC 
               
               
                   
               
               
                  94 
                 CD48 
                 NM_001778.2 
                   271- 
                 AATTTAAAGGCAGGGTCAGACTT 
               
               
                   
                   
                   
                   370 
                 GATCCTCAGAGTGGCGCACTGTA 
               
               
                   
                   
                   
                   
                 CATCTCTAAGGTCCAGAAAGAG 
               
               
                   
                   
                   
                   
                 GACAACAGCACCTACATCATGA 
               
               
                   
                   
                   
                   
                 GGGTGTTGAA 
               
               
                   
               
               
                  95 
                 CD69 
                 NM_001781.2 
                  1360- 
                 TATACAGTGTCTTACAGAGAAAA 
               
               
                   
                   
                   
                  1459 
                 GACATAAGCAAAGACTATGAGG 
               
               
                   
                   
                   
                   
                 AATATTTGCAAGACATAGAATAG 
               
               
                   
                   
                   
                   
                 TGTTGGAAAATGTGCAATATGTG 
               
               
                   
                   
                   
                   
                 ATGTGGCAA 
               
               
                   
               
               
                  96 
                 CD70 
                 NM_001252.2 
                   191- 
                 CCTATGGGTGCGTCCTGCGGGCT 
               
               
                   
                   
                   
                   290 
                 GCTTTGGTCCCATTGGTCGCGGG 
               
               
                   
                   
                   
                   
                 CTTGGTGATCTGCCTCGTGGTGT 
               
               
                   
                   
                   
                   
                 GCATCCAGCGCTTCGCACAGGCT 
               
               
                   
                   
                   
                   
                 CAGCAGCA 
               
               
                   
               
               
                  97 
                 CD79A 
                 NM_021601.3 
                   617- 
                 TGAAGATGAAAACCTTTATGAAG 
               
               
                   
                   
                   
                   716 
                 GCCTGAACCTGGACGACTGCTCC 
               
               
                   
                   
                   
                   
                 ATGTATGAGGACATCTCCCGGGG 
               
               
                   
                   
                   
                   
                 CCTCCAGGGCACCTACCAGGATG 
               
               
                   
                   
                   
                   
                 TGGGCAGC 
               
               
                   
               
               
                  98 
                 CD79B 
                 NM_000626.2 
                   350- 
                 GAAGCTGGAAAAGGGCCGCATG 
               
               
                   
                   
                   
                   449 
                 GAAGAGTCCCAGAACGAATCTCT 
               
               
                   
                   
                   
                   
                 CGCCACCCTCACCATCCAAGGCA 
               
               
                   
                   
                   
                   
                 TCCGGTTTGAGGACAATGGCATC 
               
               
                   
                   
                   
                   
                 TACTTCTGT 
               
               
                   
               
               
                  99 
                 CDC 
                 NM_006779.3 
                  1779- 
                 AGGGCTTTGTGGAGGACAGGCCT 
               
               
                   
                 42EP2 
                   
                  1878 
                 TGCCCTCAAGAACGTCGTACCTG 
               
               
                   
                   
                   
                   
                 ACGCTGAGCCTGTCATGAGAATG 
               
               
                   
                   
                   
                   
                 CAACAGGAGCAAACCAAGTGTT 
               
               
                   
                   
                   
                   
                 GCTGTGACA 
               
               
                   
               
               
                 100 
                 CDH5 
                 NM_001795.3 
                  3406- 
                 TCTCCCCTTCTCTGCCTCACCTG 
               
               
                   
                   
                   
                  3505 
                 GTCGCCAATCCATGCTCTCTTTC 
               
               
                   
                   
                   
                   
                 TTTTCTCTGTCTACTCCTTATCC 
               
               
                   
                   
                   
                   
                 CTTGGTTTAGAGGAACCCAAGAT 
               
               
                   
                   
                   
                   
                 GTGGCCTT 
               
               
                   
               
               
                 101 
                 CDKN1A 
                 NM_000389.2 
                  1976- 
                 CATGTGTCCTGGTTCCCGTTTCT 
               
               
                   
                   
                   
                  2075 
                 CCACCTAGACTGTAAACCTCTCG 
               
               
                   
                   
                   
                   
                 AGGGCAGGGACCACACCCTGTAC 
               
               
                   
                   
                   
                   
                 TGTTCTGTGTCTTTCACAGCTCC 
               
               
                   
                   
                   
                   
                 TCCCACAA 
               
               
                   
               
               
                 102 
                 CFD 
                 NM_001928.2 
                   860- 
                 CTGGTTGGTCTTTATTGAGCACC 
               
               
                   
                   
                   
                   959 
                 TACTATATGCAGAAGGGGAGGC 
               
               
                   
                   
                   
                   
                 CGAGGTGGGAGGATCATTGGAT 
               
               
                   
                   
                   
                   
                 CTCAGGAGTTCGAGATCAGCATG 
               
               
                   
                   
                   
                   
                 GGCCACGTAG 
               
               
                   
               
               
                 103 
                 CHCHD3 
                 NM_017812.2 
                  1173- 
                 TCCACCCTAACAAAGTAGGATGG 
               
               
                   
                   
                   
                  1272 
                 GGTTGGGGGCTAAATTAATTGGA 
               
               
                   
                   
                   
                   
                 GTGGGGCGAGGAGAGAGCCAGA 
               
               
                   
                   
                   
                   
                 AAACATAGATCCGAGGGCAGCA 
               
               
                   
                   
                   
                   
                 GTGCTGGGTG 
               
               
                   
               
               
                 104 
                 CHFR 
                 NM_018223.2 
                  2836- 
                 CGCCGCTCCCTCATGCTGCCCGG 
               
               
                   
                   
                   
                  2935 
                 GCCCTTCCTCCAAGACCCTACAG 
               
               
                   
                   
                   
                   
                 AGCCTGAGGGGCACCTTGGCTTC 
               
               
                   
                   
                   
                   
                 CGCCTGTGCTAGCTTTGCCATGT 
               
               
                   
                   
                   
                   
                 CATCTGGA 
               
               
                   
               
               
                 105 
                 CHMP5 
                 NM_016410.5 
                  1148- 
                 ACTAAGGAAATGGAATCTTAAA 
               
               
                   
                   
                   
                  1247 
                 AGTCTATGACAGTGTAACTCTAC 
               
               
                   
                   
                   
                   
                 AGTCTCAAAATGACCTGATAAAT 
               
               
                   
                   
                   
                   
                 TGATAAGACAAAGATGAGATTA 
               
               
                   
                   
                   
                   
                 TTGGGGCTGT 
               
               
                   
               
               
                 106 
                 CIAPIN 
                 NM_020313.3 
                  1816- 
                 GCATGTCTTGTAAAGAGAGGGG 
               
               
                   
                 1 
                   
                  1915 
                 ATGTGCATTTGTGTGTGATGTTG 
               
               
                   
                   
                   
                   
                 GATAGTCATCCACGCTCAGTTTG 
               
               
                   
                   
                   
                   
                 GACCATTGGAGGAACTTAGTGTC 
               
               
                   
                   
                   
                   
                 ACGCACAAA 
               
               
                   
               
               
                 107 
                 CKS2 
                 NM_001827.1 
                   228- 
                 AGACTTGGTGTCCAACAGAGTCT 
               
               
                   
                   
                   
                   327 
                 AGGCTGGGTTCATTACATGATTC 
               
               
                   
                   
                   
                   
                 ATGAGCCAGAACCACATATTCTT 
               
               
                   
                   
                   
                   
                 CTCTTTAGACGACCTCTTCCAAA 
               
               
                   
                   
                   
                   
                 AGATCAAC 
               
               
                   
               
               
                 108 
                 CLEC4A 
                 NM_194448.2 
                   389- 
                 ATTTCTACTGAATCAGCATCTTG 
               
               
                   
                   
                   
                   488 
                 GCAAGACAGTGAGAAGGACTGT 
               
               
                   
                   
                   
                   
                 GCTAGAATGGAGGCTCACCTGCT 
               
               
                   
                   
                   
                   
                 GGTGATAAACACTCAAGAAGAG 
               
               
                   
                   
                   
                   
                 CAGGATTTCA 
               
               
                   
               
               
                 109 
                 CLEC4C 
                 NM_203503.1 
                   571- 
                 TACGAGAGTATCAACAGTATCAT 
               
               
                   
                   
                   
                   670 
                 CCAAGCCTGACCTGCGTCATGGA 
               
               
                   
                   
                   
                   
                 AGGAAAGGACATAGAAGATTGG 
               
               
                   
                   
                   
                   
                 AGCTGCTGCCCAACCCCTTGGAC 
               
               
                   
                   
                   
                   
                 TTCATTTCA 
               
               
                   
               
               
                 110 
                 CLEC5A 
                 NM_013252.2 
                  3251- 
                 CCCCATCCAACCCTTAGACTCAC 
               
               
                   
                   
                   
                  3350 
                 GAACAAATCCACCTGAGATCAG 
               
               
                   
                   
                   
                   
                 CAGAGCCACCCTAGATCAGCTGA 
               
               
                   
                   
                   
                   
                 AACTCTAAGCACAAAAATAAAA 
               
               
                   
                   
                   
                   
                 ACTTATCACT 
               
               
                   
               
               
                 111 
                 CLIC3 
                 ILMN_179642 
                    99- 
                 CGTACGCCGCTACCTGGACAGCG 
               
               
                   
                   
                 3.1 
                   198 
                 CGATGCAGGAGAAAGAGTTCAA 
               
               
                   
                   
                   
                   
                 ATACACGTGTCCGCACAGCGCCG 
               
               
                   
                   
                   
                   
                 AGATCCTGGCGGCCTACCGGCCC 
               
               
                   
                   
                   
                   
                 GCCGTGCAC 
               
               
                   
               
               
                 112 
                 CLK2 
                 XM_941392.1 
                   552- 
                 GATTATAGCCGGGATCGGGGAG 
               
               
                   
                   
                   
                   651 
                 ATGCCTACTATGACACAGACTAT 
               
               
                   
                   
                   
                   
                 CGGCATTCCTATGAATATCAGCG 
               
               
                   
                   
                   
                   
                 GGAGAACAGCAGTTACCGCAGC 
               
               
                   
                   
                   
                   
                 CAGCGCAGCA 
               
               
                   
               
               
                 113 
                 CLN8 
                 NM_018941.3 
                  4486- 
                 GGCGCCAGAGCTGGGCTCTTCAA 
               
               
                   
                   
                   
                  4585 
                 CACGGCATTTAGCGCAGAAAGTC 
               
               
                   
                   
                   
                   
                 GTGGTTCAGGCAGTATGGGCCGC 
               
               
                   
                   
                   
                   
                 TGTGACAAAACACCTAAGACTG 
               
               
                   
                   
                   
                   
                 GGTAGTTTA 
               
               
                   
               
               
                 114 
                 CLPTM1 
                 NM_001294.3 
                  2389- 
                 TCTGTGTTTCCAGCCATCTCGCC 
               
               
                   
                   
                   
                  2488 
                 CTGCCAGCCCAGCACCACTGGGA 
               
               
                   
                   
                   
                   
                 ATCATGGTGAAGCTGATGCAGCG 
               
               
                   
                   
                   
                   
                 TTGCCGAGGGGGTGGGTTGGGC 
               
               
                   
                   
                   
                   
                 GGGGGTGGG 
               
               
                   
               
               
                 115 
                 CLSTN1 
                 NM_00100956 
                  4990- 
                 TTGAATACTGTTCTGTGACCCTG 
               
               
                   
                   
                 6.2 
                  5089 
                 ACTGCTAGTTCTGAGGACACTGG 
               
               
                   
                   
                   
                   
                 TGGCTGTGCTATGTGTGGCCATC 
               
               
                   
                   
                   
                   
                 CTCCATGTCCCGTCCCTGTAGCT 
               
               
                   
                   
                   
                   
                 GCTCTGTT 
               
               
                   
               
               
                 116 
                 CN 
                 CN312986.1 
                   491- 
                 AGGAAACTAAGACATGGAAAGG 
               
               
                   
                 312986 
                   
                   590 
                 TTAGGTAACTTGCCCAAGGTCGC 
               
               
                   
                   
                   
                   
                 ACAGCTAGTAAGTGGCAGACAT 
               
               
                   
                   
                   
                   
                 CCAGAGTCTCTGCTCTGCTCTTA 
               
               
                   
                   
                   
                   
                 ACTCTCACCA 
               
               
                   
               
               
                 117 
                 CNIH4 
                 NM_014184.3 
                   526- 
                 AATGACTGAAGCTGGAGAAGCC 
               
               
                   
                   
                   
                   625 
                 GTGGTTGAAGTCAGCCTACACTA 
               
               
                   
                   
                   
                   
                 CAGTGCACAGTTGAGGAGCCAG 
               
               
                   
                   
                   
                   
                 AGACTTCTTAAATCATCCTTAGA 
               
               
                   
                   
                   
                   
                 ACCGTGACCA 
               
               
                   
               
               
                 118 
                 CNPY2 
                 NM_014255.5 
                  1038- 
                 TTGCAGTAAGCGAACAGATCTTT 
               
               
                   
                   
                   
                  1137 
                 GTGACCATGCCCTGCACATATCG 
               
               
                   
                   
                   
                   
                 CATGATGAGCTATGAACCACTGG 
               
               
                   
                   
                   
                   
                 AGCAGCCCACACTGGCTTGATGG 
               
               
                   
                   
                   
                   
                 ATCACCCC 
               
               
                   
               
               
                 119 
                 COLEC 
                 NM_130386.2 
                   901- 
                 ACACAAGCCAGGCTATCCAGCG 
               
               
                   
                 12 
                   
                  1000 
                 AATCAAGAACGACTTTCAAAATC 
               
               
                   
                   
                   
                   
                 TGCAGCAGGTTTTTCTTCAAGCC 
               
               
                   
                   
                   
                   
                 AAGAAGGACACGGATTGGCTGA 
               
               
                   
                   
                   
                   
                 AGGAGAAAGT 
               
               
                   
               
               
                 120 
                 GLT25D 
                 NM_024656.2 
                  3067- 
                 CTGTGTGCCAGGCCTCACAGACT 
               
               
                   
                 1 
                   
                  3166 
                 CCCAGTTGGGTTGAAGAATGGTT 
               
               
                   
                   
                   
                   
                 GACTGAGTTTGATTCTTCCTGTA 
               
               
                   
                   
                   
                   
                 CCCTCGGTCGTCTGAGCTGTGTG 
               
               
                   
                   
                   
                   
                 CGGACAAC 
               
               
                   
               
               
                 121 
                 COMMD6 
                 NM_203497.3 
                    32- 
                 CTCTCGAGTCCGGGCCGCAAGTC 
               
               
                   
                   
                   
                   131 
                 CCAGACGCTGCCCATGGAGGCGT 
               
               
                   
                   
                   
                   
                 CCAGCGAGCCGCCGCTGGATGCT 
               
               
                   
                   
                   
                   
                 AAGTCCGATGTCACCAACCAGCT 
               
               
                   
                   
                   
                   
                 TGTAGATT 
               
               
                   
               
               
                 122 
                 CORO1C 
                 ILMN_174595 
                    98- 
                 AAGTAAAGTTGTTGATGGTGGTG 
               
               
                   
                   
                 4.1 
                   197 
                 AAACACCGTAGGGCATGTGGTTC 
               
               
                   
                   
                   
                   
                 AAAGAGAAGCAGGAGGGCAAGG 
               
               
                   
                   
                   
                   
                 GAAAGTTACCCTGATCTTAGTTT 
               
               
                   
                   
                   
                   
                 GTAGCTTAT 
               
               
                   
               
               
                 123 
                 COX6C 
                 NM_004374.2 
                    70- 
                 GAAGTTTTGCCAAAACCTCGGAT 
               
               
                   
                   
                   
                   169 
                 GCGTGGCCTTCTGGCCAGGCGTC 
               
               
                   
                   
                   
                   
                 TGCGAAATCATATGGCTGTAGCA 
               
               
                   
                   
                   
                   
                 TTCGTGCTATCCCTGGGGGTTGC 
               
               
                   
                   
                   
                   
                 AGCTTTGT 
               
               
                   
               
               
                 124 
                 COX7B 
                 NM_001866.2 
                   160- 
                 CAGAGCCACCAGAAACGTACAC 
               
               
                   
                   
                   
                   259 
                 CTGATTTTCATGACAAATACGGT 
               
               
                   
                   
                   
                   
                 AATGCTGTATTAGCTAGTGGAGC 
               
               
                   
                   
                   
                   
                 CACTTTCTGTATTGTTACATGGA 
               
               
                   
                   
                   
                   
                 CATATGTAG 
               
               
                   
               
               
                 125 
                 COX7C 
                 NM_001867.2 
                     1- 
                 CAAGGTCGTGAAAAAAAAGGTC 
               
               
                   
                   
                   
                   100 
                 TTGGTGAGGTGCCGCCATTTCAT 
               
               
                   
                   
                   
                   
                 CTGTCCTCATTCTCTGCGCCTTT 
               
               
                   
                   
                   
                   
                 CGCAGAGCTTCCAGCAGCGGTAT 
               
               
                   
                   
                   
                   
                 GTTGGGCCA 
               
               
                   
               
               
                 126 
                 CPPED1 
                 NM_018340.2 
                  2494- 
                 TGTATTTGTTTCTTTACAACAGG 
               
               
                   
                   
                   
                  2593 
                 TGTAGGTATAGGAGGTCAAGAAA 
               
               
                   
                   
                   
                   
                 AGGAGTTCGGTAAAGGGCATAG 
               
               
                   
                   
                   
                   
                 CTAATAACAACCACACATTGGGC 
               
               
                   
                   
                   
                   
                 CAGGCACAG 
               
               
                   
               
               
                 127 
                 CR2 b 
                 NM_00100665 
                   486- 
                 GGTGTCAAGCAAATAATATGTGG 
               
               
                   
                   
                 8.1 
                   585 
                 GGGCCGACACGACTACCAACCT 
               
               
                   
                   
                   
                   
                 GTGTAAGTGTTTTCCCTCTCGAG 
               
               
                   
                   
                   
                   
                 TGTCCAGCACTTCCTATGATCCA 
               
               
                   
                   
                   
                   
                 CAATGGACA 
               
               
                   
               
               
                 128 
                 CR2 
                 NM_00100665 
                  3581- 
                 AGCCCAGTTTCACTGCCATATAC 
               
               
                   
                   
                 8.2 
                  3680 
                 TCTTCAAGGACTTTCTGAAGCCT 
               
               
                   
                   
                   
                   
                 CACTTATGAGATGCCTGAAGCCA 
               
               
                   
                   
                   
                   
                 GGCCATGGCTATAAACAATTACA 
               
               
                   
                   
                   
                   
                 TGGCTCTA 
               
               
                   
               
               
                 129 
                 CREB1 
                 NM_004379.3 
                  4856- 
                 TTTGATGGTAGGTCAGCAGCAGT 
               
               
                   
                   
                   
                  4955 
                 GCTAGTCTCTGAAAGCACAATAC 
               
               
                   
                   
                   
                   
                 CAGTCAGGCAGCCTATCCCATCA 
               
               
                   
                   
                   
                   
                 GATGTCATCTGGCTGAAGTTTAT 
               
               
                   
                   
                   
                   
                 CTCTGTCT 
               
               
                   
               
               
                 130 
                 CREB5 
                 NM_182898.3 
                  7898- 
                 ACCTACTCACCTTTTTCCCTTCT 
               
               
                   
                   
                   
                  7997 
                 AAGTTCTGCTAAATCACATCTGC 
               
               
                   
                   
                   
                   
                 CTCATAGAGAAAGGAATGTTGCC 
               
               
                   
                   
                   
                   
                 TTTGAGAACTGTCTTGGAGAACA 
               
               
                   
                   
                   
                   
                 GATAAGCT 
               
               
                   
               
               
                 131 
                 CRKL 
                 NM_005207.3 
                  4901- 
                 TTCTAAAGGAGCAGAAGGACAG 
               
               
                   
                   
                   
                  5000 
                 GTCTCTGAGACAGGATCGTTGTC 
               
               
                   
                   
                   
                   
                 CCTACAGGAGGAACAGTGGCCTT 
               
               
                   
                   
                   
                   
                 GCTTCTTAGACGGTCTTCACTGT 
               
               
                   
                   
                   
                   
                 GTGTTTTAA 
               
               
                   
               
               
                 132 
                 CRY2 
                 NM_021117.3 
                  4013- 
                 CAGCTCAGGTGGCCCTGAGGGCT 
               
               
                   
                   
                   
                  4112 
                 CCCTCGGAACAGTGCCTCAAATC 
               
               
                   
                   
                   
                   
                 CTGACCCAAGGGCCAGCATGGG 
               
               
                   
                   
                   
                   
                 GAAGAGATGGTTGCAGGCAAAA 
               
               
                   
                   
                   
                   
                 TGCACTTTAT 
               
               
                   
               
               
                 133 
                 CS 
                 NM_004077.2 
                  2080- 
                 CCTCCTAGCAAGACCTGTTGGTT 
               
               
                   
                   
                   
                  2179 
                 AGCTGGACATGCTTTGGCAATTT 
               
               
                   
                   
                   
                   
                 TTTTATACTACCAAGTGACCATA 
               
               
                   
                   
                   
                   
                 AAGGCATGGCATTTGTTGTGACT 
               
               
                   
                   
                   
                   
                 GGCACCCA 
               
               
                   
               
               
                 134 
                 CSK 
                 NM_004383.2 
                  2501- 
                 TCTAGGGACCCCTCGCCCCAGCC 
               
               
                   
                   
                   
                  2600 
                 TCATTCCCCATTCTGTGTCCCAT 
               
               
                   
                   
                   
                   
                 GTCCCGTGTCTCCTCGGTCGCCC 
               
               
                   
                   
                   
                   
                 CGTGTTTGCGCTTGACCATGTTG 
               
               
                   
                   
                   
                   
                 CACTGTTT 
               
               
                   
               
               
                 135 
                 CST7 
                 NM_003650.3 
                   618- 
                 CAACCACACCTTGAAGCAGACTC 
               
               
                   
                   
                   
                   717 
                 TGAGCTGCTACTCTGAAGTCTGG 
               
               
                   
                   
                   
                   
                 GTCGTGCCCTGGCTCCAGCACTT 
               
               
                   
                   
                   
                   
                 CGAGGTGCCTGTTCTCCGTTGTC 
               
               
                   
                   
                   
                   
                 ACTGACCC 
               
               
                   
               
               
                 136 
                 CTAG1B 
                 NM_001327.2 
                   286- 
                 GCGGGGCCAGGGGGCCGGAGAG 
               
               
                   
                   
                   
                   385 
                 CCGCCTGCTTGAGTTCTACCTCG 
               
               
                   
                   
                   
                   
                 CCATGCCTTTCGCGACACCCATG 
               
               
                   
                   
                   
                   
                 GAAGCAGAGCTGGCCCGCAGGA 
               
               
                   
                   
                   
                   
                 GCCTGGCCCA 
               
               
                   
               
               
                 137 
                 CTDSP2 
                 NM_005730.3 
                  4685- 
                 GAGGTCGGGCCAGCTGCCCCATT 
               
               
                   
                   
                   
                  4784 
                 CTTTTAACGTTGTAGGGCCTGCC 
               
               
                   
                   
                   
                   
                 CATGGAGCGGACCCTCCTCTTTG 
               
               
                   
                   
                   
                   
                 GGCCTCGTGAGCTTTTTTGCTTA 
               
               
                   
                   
                   
                   
                 TCATGTTC 
               
               
                   
               
               
                 138 
                 CTSW 
                 NM_001335.3 
                  1076- 
                 TGCACCGAGGGAGCAATACCTGT 
               
               
                   
                   
                   
                  1175 
                 GGCATCACCAAGTTCCCGCTCAC 
               
               
                   
                   
                   
                   
                 TGCCCGTGTGCAGAAACCGGATA 
               
               
                   
                   
                   
                   
                 TGAAGCCCCGAGTCTCCTGCCCT 
               
               
                   
                   
                   
                   
                 CCCTGAAC 
               
               
                   
               
               
                 139 
                 CTSZ 
                 NM_001336.3 
                  1174- 
                 CACTGGCTGCGAGTGTTCCTGAG 
               
               
                   
                   
                   
                  1273 
                 AGTTGAAAGTGGGATGACTTATG 
               
               
                   
                   
                   
                   
                 ACACTTGCACAGCATGGCTCTGC 
               
               
                   
                   
                   
                   
                 CTCACAATGATGCAGTCAGCCAC 
               
               
                   
                   
                   
                   
                 CTGGTGAA 
               
               
                   
               
               
                 140 
                 CX3CL1 
                 NM_002996.3 
                   141- 
                 AGCACCACGGTGTGACGAAATG 
               
               
                   
                   
                   
                   240 
                 CAACATCACGTGCAGCAAGATG 
               
               
                   
                   
                   
                   
                 ACATCAAAGATACCTGTAGCTTT 
               
               
                   
                   
                   
                   
                 GCTCATCCACTATCAACAGAACC 
               
               
                   
                   
                   
                   
                 AGGCATCATG 
               
               
                   
               
               
                 141 
                 CXCL2 
                 NM_002089.3 
                   855- 
                 ATCACATGTCAGCCACTGTGATA 
               
               
                   
                   
                   
                   954 
                 GAGGCTGAGGAATCCAAGAAAA 
               
               
                   
                   
                   
                   
                 TGGCCAGTGAGATCAATGTGACG 
               
               
                   
                   
                   
                   
                 GCAGGGAAATGTATGTGTGTCTA 
               
               
                   
                   
                   
                   
                 TTTTGTAAC 
               
               
                   
               
               
                 142 
                 IL8RB 
                 NM_001557.3 
                   410- 
                 ACCTCAAAAATGGAAGATTTTAA 
               
               
                   
                   
                   
                   509 
                 CATGGAGAGTGACAGCTTTGAA 
               
               
                   
                   
                   
                   
                 GATTTCTGGAAAGGTGAAGATCT 
               
               
                   
                   
                   
                   
                 TAGTAATTACAGTTACAGCTCTA 
               
               
                   
                   
                   
                   
                 CCCTGCCCC 
               
               
                   
               
               
                 143 
                 CXCR5  
                 NM_001716.3 
                  2619- 
                 ACGTCCCTTTTTTCTCTGAGTAT 
               
               
                   
                 b 
                   
                  2718 
                 CTCCTCGCAAGCTGGGTAATCGA 
               
               
                   
                   
                   
                   
                 TGGGGGAGTCTGAAGCAGATGCA 
               
               
                   
                   
                   
                   
                 AAGAGGCAAGAGGCTGGATTTT 
               
               
                   
                   
                   
                   
                 GAATTTTCT 
               
               
                   
               
               
                 144 
                 CYBB 
                 NM_000397.3 
                  3787- 
                 ACTGGAGAGGGTACCTCAGTTAT 
               
               
                   
                   
                   
                  3886 
                 AAGGAGTCTGAGAATATTGGCCC 
               
               
                   
                   
                   
                   
                 TTTCTAACCTATGTGCATAATTA 
               
               
                   
                   
                   
                   
                 AAACCAGCTTCATTTGTTGCTCC 
               
               
                   
                   
                   
                   
                 GAGAGTGT 
               
               
                   
               
               
                 145 
                 CYP1B1 
                 NM_000104.3 
                  2361- 
                 CTTACACCAAACTACTGAATGAA 
               
               
                   
                   
                   
                  2460 
                 GCAGTATTTTGGTAACCAGGCCA 
               
               
                   
                   
                   
                   
                 TTTTTGGTGGGAATCCAAGATTG 
               
               
                   
                   
                   
                   
                 GTCTCCCATATGCAGAAATAGAC 
               
               
                   
                   
                   
                   
                 AAAAAGTA 
               
               
                   
               
               
                 146 
                 DB 
                 DB338252.1 
                   436- 
                 GTTCTTGGTCTGTATGTGTAGGT 
               
               
                   
                 338252 
                   
                   535 
                 GGAGGGAGGCAAAGTTGTGGTA 
               
               
                   
                   
                   
                   
                 ATAAAGTGGGAAGGCCCGGGAA 
               
               
                   
                   
                   
                   
                 GAACAGCTAACTGTATAGGGGT 
               
               
                   
                   
                   
                   
                 GAAATGACGCT 
               
               
                   
               
               
                 147 
                 DBI 
                 NM_00107986 
                   241- 
                 CATAAATACAGAACGGCCCGGG 
               
               
                   
                   
                 2.1 
                   340 
                 ATGTTGGACTTCACGGGCAAGGC 
               
               
                   
                   
                   
                   
                 CAAGTGGGATGCCTGGAATGAG 
               
               
                   
                   
                   
                   
                 CTGAAAGGGACTTCCAAGGAAG 
               
               
                   
                   
                   
                   
                 ATGCCATGAAA 
               
               
                   
               
               
                 148 
                 DCAF7 
                 NM_005828.4 
                  6155- 
                 TTAACACTGTGCTGTGAAACAAC 
               
               
                   
                   
                   
                  6254 
                 TATGGGGAATCTCCATTGAAGGC 
               
               
                   
                   
                   
                   
                 TACTTCATGGGCACCTGAAAGTG 
               
               
                   
                   
                   
                   
                 GAGTGTTATAGCTATGACTTTCT 
               
               
                   
                   
                   
                   
                 ATTTCTTG 
               
               
                   
               
               
                 149 
                 DDIT4 
                 NM_019058.2 
                  1414- 
                 GACCTGTTGTAGGCAGCTATCTT 
               
               
                   
                   
                   
                  1513 
                 ACAGACGCATGAATGTAAGAGT 
               
               
                   
                   
                   
                   
                 AGGAAGGGGTGGGTGTCAGGGA 
               
               
                   
                   
                   
                   
                 TCACTTGGGATCTTTGACACTTG 
               
               
                   
                   
                   
                   
                 AAAAATTACA 
               
               
                   
               
               
                 150 
                 DDX23 
                 NM_004818.2 
                  2811- 
                 ATTGCACTGGGCCATCAGCTCAT 
               
               
                   
                   
                   
                  2910 
                 GCCAGGCTATGGGGGCAGCCAG 
               
               
                   
                   
                   
                   
                 TTGGCATTGCTCCCCAGACTGAA 
               
               
                   
                   
                   
                   
                 CAGAAACCTGGCCGCCGGATGG 
               
               
                   
                   
                   
                   
                 GACCTCCTTT 
               
               
                   
               
               
                 151 
                 DGUOK 
                 NM_080916.2 
                   573- 
                 ACATCGAGTGGCATATCTATCAG 
               
               
                   
                   
                   
                   672 
                 GACTGGCATTCTTTTCTCCTGTG 
               
               
                   
                   
                   
                   
                 GGAGTTTGCCAGCCGGATCACAT 
               
               
                   
                   
                   
                   
                 TACATGGCTTCATCTACCTCCAG 
               
               
                   
                   
                   
                   
                 GCTTCTCC 
               
               
                   
               
               
                 152 
                 DGUOK  
                 NM_080916.2 
                   903- 
                 TTGTAAAGAATCTGTAACCAATA 
               
               
                   
                 b 
                   
                  1002 
                 CCATGAAGTTCAGGCTGTGATCT 
               
               
                   
                   
                   
                   
                 GGGCTCCCTGACTTTCTGAAGCT 
               
               
                   
                   
                   
                   
                 AGAAAAATGTTGTGTCTCCCAAC 
               
               
                   
                   
                   
                   
                 CACCTTTC 
               
               
                   
               
               
                 153 
                 DHX16  
                 NM_00116423 
                  2491- 
                 CCCGTGTCAACTTCTTTCTCCCT 
               
               
                   
                 b 
                 9.1 
                  2590 
                 GGCGGTGACCACCTGGTTCTGCT 
               
               
                   
                   
                   
                   
                 AAATGTTTACACACAGTGGGCTG 
               
               
                   
                   
                   
                   
                 AGAGTGGTTACTCTTCCCAGTGG 
               
               
                   
                   
                   
                   
                 TGCTATGA 
               
               
                   
               
               
                 154 
                 DHX16 
                 NM_003587.4 
                  3189- 
                 ACCAAAGAGTTCATGAGACAGG 
               
               
                   
                   
                   
                  3288 
                 TACTGGAGATTGAGAGCAGTTGG 
               
               
                   
                   
                   
                   
                 CTTCTGGAGGTGGCTCCCCATTA 
               
               
                   
                   
                   
                   
                 TTATAAGGCCAAGGAGCTAGAA 
               
               
                   
                   
                   
                   
                 GATCCCCATG 
               
               
                   
               
               
                 155 
                 DKFZp 
                 XM_291277.4 
                  4192- 
                 CTCCTGCAGCTTCTGTGAGCCAA 
               
               
                   
                 761PO4 
                   
                  4291 
                 GCCCCAGCCTGCACCGTCGCTGC 
               
               
                   
                 23 
                   
                   
                 CCCTTCCCTGCCTAACCCTTTCC 
               
               
                   
                   
                   
                   
                 TGTCTCGCCTTGGAAGCACCCAT 
               
               
                   
                   
                   
                   
                 GTCTCCCT 
               
               
                   
               
               
                 156 
                 DMBT1 
                 NM_007329.2 
                  3713- 
                 CACAATGGCTGGCTCTCCCACAA 
               
               
                   
                   
                   
                  3812 
                 CTGTGGCCATCATGAAGACGCTG 
               
               
                   
                   
                   
                   
                 GTGTCATCTGCTCAGCTTCCCAG 
               
               
                   
                   
                   
                   
                 TCCCAGCCGACACCCAGCCCAGA 
               
               
                   
                   
                   
                   
                 CACTTGGC 
               
               
                   
               
               
                 157 
                 DNAJB1 
                 NM_006145.2 
                  1904- 
                 GACCTCTGGCTCCAGTGAAGCTG 
               
               
                   
                   
                   
                  2003 
                 AATGTCCTCACTTTGTGGGTCAC 
               
               
                   
                   
                   
                   
                 ACTCTTTACATTTCTGTAAGGCA 
               
               
                   
                   
                   
                   
                 ATCTTGGCACACGTGGGGCTTAC 
               
               
                   
                   
                   
                   
                 CAGTGGCC 
               
               
                   
               
               
                 158 
                 DNAJB6 
                 NM_058246.3 
                  2087- 
                 CTTCCCTGCATGCTCCCTCCCAG 
               
               
                   
                   
                   
                  2186 
                 TGACTTTCCTTCCCTTTCACATG 
               
               
                   
                   
                   
                   
                 AGGATCTGCCGTTCATGTTGCTT 
               
               
                   
                   
                   
                   
                 TCTCCTTTGTCCTCTTGGACTTG 
               
               
                   
                   
                   
                   
                 AGGGCATT 
               
               
                   
               
               
                 159 
                 DOCK5 
                 NM_024940.6 
                  7201- 
                 AAAGAGATTTCCATTTCTGCTGC 
               
               
                   
                   
                   
                  7300 
                 CAGAGCTGGTATTTGCCTGCCTG 
               
               
                   
                   
                   
                   
                 ATTCTCTGTGTTTCCTGTTTCAC 
               
               
                   
                   
                   
                   
                 CGCCACCCTTTCAGGAGAGAACT 
               
               
                   
                   
                   
                   
                 ACACCAGT 
               
               
                   
               
               
                 160 
                 DPF2 
                 NM_006268.4 
                  2249- 
                 TCTCAGCTCATGGGGAAGCCACA 
               
               
                   
                   
                   
                  2348 
                 TAGACATCCCTTTCTTCCCTTGC 
               
               
                   
                   
                   
                   
                 ACGCTCGCTAGCAGCTGGTAAGG 
               
               
                   
                   
                   
                   
                 TCTTCACACCCTGATTCCTCAAG 
               
               
                   
                   
                   
                   
                 TTTTCTGC 
               
               
                   
               
               
                 161 
                 DYNC2 
                 NM_016008.3 
                   351- 
                 TTTGGGAACTCGGTGGAGGAACC 
               
               
                   
                 LI1 
                   
                   450 
                 TCTTTATTGGACTTAATCAGCAT 
               
               
                   
                   
                   
                   
                 ACCCATCACAGGTGACACCTTAC 
               
               
                   
                   
                   
                   
                 GGACGTTTTCTCTTGTTCTCGTT 
               
               
                   
                   
                   
                   
                 CTGGATCT 
               
               
                   
               
               
                 162 
                 DZIP3 
                 NM_014648.3 
                  4323- 
                 CCCAGTGTCTTGCCCAGTAGATA 
               
               
                   
                   
                   
                  4422 
                 CAAGATAAATATTGCCAGAATCA 
               
               
                   
                   
                   
                   
                 GATATCAGGAAGTAGTAAGAAA 
               
               
                   
                   
                   
                   
                 AGGAGTTAATATGCAAACTAAAT 
               
               
                   
                   
                   
                   
                 CACTCGCTC 
               
               
                   
               
               
                 163 
                 EEF1B2 
                 NM_00103766 
                   699- 
                 GGATACGGAATTAAGAAACTTC 
               
               
                   
                   
                 3.1 
                   798 
                 AAATACAGTGTGTAGTTGAAGAT 
               
               
                   
                   
                   
                   
                 GATAAAGTTGGAACAGATATGCT 
               
               
                   
                   
                   
                   
                 GGAGGAGCAGATCACTGCTTTTG 
               
               
                   
                   
                   
                   
                 AGGACTATG 
               
               
                   
               
               
                 164 
                 EGLN1 
                 NM_022051.1 
                  3976- 
                 AGCAGCATGGACGACCTGATAC 
               
               
                   
                   
                   
                  4075 
                 GCCACTGTAACGGGAAGCTGGG 
               
               
                   
                   
                   
                   
                 CAGCTACAAAATCAATGGCCGG 
               
               
                   
                   
                   
                   
                 ACGAAAGCCATGGT 
               
               
                   
               
               
                 165 
                 EGR1 
                 NM_001964.2 
                  1506- 
                 GAGGCATACCAAGATCCACTTGC 
               
               
                   
                   
                   
                  1605 
                 GGCAGAAGGACAAGAAAGCAGA 
               
               
                   
                   
                   
                   
                 CAAAAGTGTTGTGGCCTCTTCGG 
               
               
                   
                   
                   
                   
                 CCACCTCCTCTCTCTCTTCCTAC 
               
               
                   
                   
                   
                   
                 CCGTCCCCG 
               
               
                   
               
               
                 166 
                 EHD4 
                 NM_139265.3 
                  2605- 
                 TCAAACATTAAATATCCCGAGGT 
               
               
                   
                   
                   
                  2704 
                 CTCCTTGGTGGGTGGCAGGATTT 
               
               
                   
                   
                   
                   
                 AAATTCAATCAAATCCTGTCCTA 
               
               
                   
                   
                   
                   
                 GTGTGTGCAGTGTCTTCGGCCCT 
               
               
                   
                   
                   
                   
                 GTGGACAC 
               
               
                   
               
               
                 167 
                 EID2B 
                 NM_152361.2 
                   628- 
                 GCCAGTTTAGTTAACTCAGTCAT 
               
               
                   
                   
                   
                   727 
                 TAGGGGGAATGCAAACTGGAAG 
               
               
                   
                   
                   
                   
                 GGAATACGGCAATGTGCAATTG 
               
               
                   
                   
                   
                   
                 AAGGAGGAAGCACACTCCGAAA 
               
               
                   
                   
                   
                   
                 TGGAAACAGAC 
               
               
                   
               
               
                 168 
                 EIF2B4 
                 NM_015636.3 
                  1497- 
                 GTCTCTAATGAGCTAGATGACCC 
               
               
                   
                   
                   
                  1596 
                 TGATGATCTGCAATGTAAGCGGG 
               
               
                   
                   
                   
                   
                 GAGAACATGTTGCGCTGGCTAAC 
               
               
                   
                   
                   
                   
                 TGGCAGAACCACGCATCCCTACG 
               
               
                   
                   
                   
                   
                 GTTGTTGA 
               
               
                   
               
               
                 169 
                 EIF4EN 
                 NM_019843.2 
                  3051- 
                 CACACTGGGCAGGACCCTGCTTC 
               
               
                   
                 IF1 
                   
                  3150 
                 ATCTCGGGTTGGTTTATGGGCTT 
               
               
                   
                   
                   
                   
                 TTACTTTGGAGCACTCTGTGTGA 
               
               
                   
                   
                   
                   
                 AGCTGTTTGGTGGAACCCATGCA 
               
               
                   
                   
                   
                   
                 TCTGGTGT 
               
               
                   
               
               
                 170 
                 EMR4 
                 NM_00108049 
                  1719- 
                 GGGAAGACGATTGGATCAATCA 
               
               
                   
                   
                 8.2 
                  1818 
                 TTGCATACTCATTCACCATCATC 
               
               
                   
                   
                   
                   
                 AACACCCTTCAGGGAGTGTTGCT 
               
               
                   
                   
                   
                   
                 CTTTGTGGTACACTGTCTCCTTA 
               
               
                   
                   
                   
                   
                 ATCGCCAGG 
               
               
                   
               
               
                 171 
                 EP300 
                 NM_001429.2 
                   716- 
                 CCAGCCAGGCCCAACAGAGCAG 
               
               
                   
                   
                   
                   815 
                 TCCTGGATTAGGTTTGATAAATA 
               
               
                   
                   
                   
                   
                 GCATGGTCAAAAGCCCAATGAC 
               
               
                   
                   
                   
                   
                 ACAGGCAGGCTTGACTTCTCCCA 
               
               
                   
                   
                   
                   
                 ACATGGGGAT 
               
               
                   
               
               
                 172 
                 EPHX2 
                 NM_001979.5 
                  1909- 
                 CATCCTTCCACCTGCTGGGGCAC 
               
               
                   
                   
                   
                  2008 
                 CATTCTTAGTATACAGAGGTGGC 
               
               
                   
                   
                   
                   
                 CTTACACACATCTTGCATGGATG 
               
               
                   
                   
                   
                   
                 GCAGCATTGTTCTGAAGGGGTTT 
               
               
                   
                   
                   
                   
                 GCAGAAAA 
               
               
                   
               
               
                 173 
                 ERLIN1 
                 NM_006459.3 
                  3197- 
                 TGATGGCCCTGGAGGCGGGGCT 
               
               
                   
                   
                   
                  3296 
                 GAGGAACAGGGAAATGCCGCTG 
               
               
                   
                   
                   
                   
                 TGAAGTCTTAAAGCACTTCTGCT 
               
               
                   
                   
                   
                   
                 TAAACTCCCATGTGTGAGGAGTG 
               
               
                   
                   
                   
                   
                 TGCCTCCCTG 
               
               
                   
               
               
                 174 
                 ETFDH 
                 NM_004453.3 
                  1904- 
                 TGACCTCTTGTCATCTGTGGCTC 
               
               
                   
                   
                   
                  2003 
                 TGAGTGGTACTAATCATGAACAT 
               
               
                   
                   
                   
                   
                 GACCAGCCGGCACACTTAACCTT 
               
               
                   
                   
                   
                   
                 AAGGGATGACAGTATACCTGTAA 
               
               
                   
                   
                   
                   
                 ATAGAAAT 
               
               
                   
               
               
                 175 
                 EVI2A 
                 NM_014210.3 
                  1410- 
                 GAGAGAGCTAAACTGTGTAATTT 
               
               
                   
                   
                   
                  1509 
                 AATGGTATCTTCCTTGCTGGATG 
               
               
                   
                   
                   
                   
                 TGGCAGAATCCACACCAGCTTAT 
               
               
                   
                   
                   
                   
                 CAACCAACACAGCTAATTTTAGA 
               
               
                   
                   
                   
                   
                 ATAGATCC 
               
               
                   
               
               
                 176 
                 EWSR1 
                 NM_005243.3 
                  2248- 
                 AAAAATGGATAAAGGCGAGCAC 
               
               
                   
                   
                   
                  2347 
                 CGTCAGGAGCGCAGAGATCGGC 
               
               
                   
                   
                   
                   
                 CCTACTAGATGCAGAGACCCCGC 
               
               
                   
                   
                   
                   
                 AGAGCTGCATTGACTACCAGATT 
               
               
                   
                   
                   
                   
                 TATTTTTTAA 
               
               
                   
               
               
                 177 
                 EYA3 
                 NM_001990.3 
                  1551- 
                 GATTCCTGGTTAGGAACTGCATT 
               
               
                   
                   
                   
                  1650 
                 AAAGTCCTTACTTCTCATCCAGT 
               
               
                   
                   
                   
                   
                 CCAGAAAGAATTGTGTGAATGTT 
               
               
                   
                   
                   
                   
                 CTGATCACTACCACCCAGCTGGT 
               
               
                   
                   
                   
                   
                 TCCAGCCC 
               
               
                   
               
               
                 178 
                 C5orf 
                 NM_032042.5 
                  4058- 
                 TTAGAACAAGTAGAATGGGAAA 
               
               
                   
                 21 
                   
                  4157 
                 GGAGTGACTGATAAATCTAAGAT 
               
               
                   
                   
                   
                   
                 TCAAAATAGTCCCGTCGAAACTT 
               
               
                   
                   
                   
                   
                 AAAGGCCAGATTATTGCTTTGGA 
               
               
                   
                   
                   
                   
                 GCTTTCTAT 
               
               
                   
               
               
                 179 
                 FAM179 
                 NM_199280.2 
                  3306- 
                 ACTCTTAGACTCAGAGTCCTTGG 
               
               
                   
                 A 
                   
                  3405 
                 GAGGCAGCCGCAAGGCCACTGA 
               
               
                   
                   
                   
                   
                 CAGAGGGGTGGCCCCTGACAGC 
               
               
                   
                   
                   
                   
                 AAGACAACTGGCAGCTCATACCC 
               
               
                   
                   
                   
                   
                 TTTTCAGCTG 
               
               
                   
               
               
                 180 
                 FAM193 
                 NM_003704.3 
                  4523- 
                 CCCTGACTTGTAGCCAGCTTGTG 
               
               
                   
                 A 
                   
                  4622 
                 TAAGATCCCTTGCAGAACGAGA 
               
               
                   
                   
                   
                   
                 AAGTTAAAAACAAGCCCACCCA 
               
               
                   
                   
                   
                   
                 GTACTCACACCATCAAGTCTGTT 
               
               
                   
                   
                   
                   
                 ATAGAGTGTA 
               
               
                   
               
               
                 181 
                 FAM43A 
                 NM_153690.4 
                  2741- 
                 AGACCCCTGAAATGTTGCCAAAT 
               
               
                   
                   
                   
                  2840 
                 TCTTCAAATAACTGTTTGGGGGG 
               
               
                   
                   
                   
                   
                 TGGGGGGAGATGAAAGAGAGTC 
               
               
                   
                   
                   
                   
                 GCGTTTTGTTTACAGTTAAAGAC 
               
               
                   
                   
                   
                   
                 ATCCAATAT 
               
               
                   
               
               
                 182 
                 FAM50B 
                 NM_012135.1 
                  1273- 
                 TTCTGAGTATTTTAGTGTTGCCA 
               
               
                   
                   
                   
                  1372 
                 CCTGGATTTGCTGCATTGCTCTG 
               
               
                   
                   
                   
                   
                 CTGAGCTGTATTGAAACCATGAC 
               
               
                   
                   
                   
                   
                 TGGGCCCACTGTCAGACAGAAAT 
               
               
                   
                   
                   
                   
                 TAGAATAG 
               
               
                   
               
               
                 183 
                 FAIM3 
                 NM_005449.4 
                  1689- 
                 CAGGCTCTAGATCACATGGCATC 
               
               
                   
                   
                   
                  1788 
                 AGGCTGGGGCAGAGGCATAGCT 
               
               
                   
                   
                   
                   
                 ATTGTCTCGGGCATCCTTCCCAG 
               
               
                   
                   
                   
                   
                 GGTTGGGTCTTACACAAATAGAA 
               
               
                   
                   
                   
                   
                 GGCTCTTGC 
               
               
                   
               
               
                 184 
                 FKBP1A 
                 NM_054014.3 
                   301- 
                 AGAAACAAGCCCTTTAAGTTTAT 
               
               
                   
                   
                   
                   400 
                 GCTAGGCAAGCAGGAGGTGATC 
               
               
                   
                   
                   
                   
                 CGAGGCTGGGAAGAAGGGGTTG 
               
               
                   
                   
                   
                   
                 CCCAGATGAGTGTGGGTCAGAG 
               
               
                   
                   
                   
                   
                 AGCCAAACTGA 
               
               
                   
               
               
                 185 
                 FLNB 
                 NM_001457.3 
                  9148- 
                 CAGACCTGAGCTGGCTTTGGAAT 
               
               
                   
                   
                   
                  9247 
                 GAGGTTAAAGTGTCAGGGACGTT 
               
               
                   
                   
                   
                   
                 GCCTGAGCCCAAATGTGTAGTGT 
               
               
                   
                   
                   
                   
                 GGTCTGGGCAGGCAGACCTTTAG 
               
               
                   
                   
                   
                   
                 GTTTTGCT 
               
               
                   
               
               
                 186 
                 FNBP1 
                 NM_015033.2 
                  5237- 
                 TGTGTGTTGCACTAATTCTAAAC 
               
               
                   
                   
                   
                  5336 
                 TTTGGAGGCATTTTGCTGTGTGA 
               
               
                   
                   
                   
                   
                 GGCCGATCGCCACTGTAAAGGTC 
               
               
                   
                   
                   
                   
                 CTAGAGTTGCCTGTTTGTCTCTG 
               
               
                   
                   
                   
                   
                 GAGATGGA 
               
               
                   
               
               
                 187 
                 FOXK2 
                 NM_004514.3 
                  4387- 
                 TTTTTTGCCGTAGGCACCATTCT 
               
               
                   
                   
                   
                  4486 
                 GCATCTTGAACCCAGACTGAAGT 
               
               
                   
                   
                   
                   
                 GTGCCTCTCACAGATGGAAGGTG 
               
               
                   
                   
                   
                   
                 CACACGCTCCTGTCTCCTCCTCA 
               
               
                   
                   
                   
                   
                 CTCTGCCA 
               
               
                   
               
               
                 188 
                 FRAT2 
                 NM_012083.2 
                  1769- 
                 CTTGTCCTCCCAGCTGAGCTTTC 
               
               
                   
                   
                   
                  1868 
                 TTATTCCACCCTTTCTGGTGTCT 
               
               
                   
                   
                   
                   
                 ATAGGAATGCATGAGAGACCCTG 
               
               
                   
                   
                   
                   
                 GACGTTTTTCTGCTCTCTTCTGG 
               
               
                   
                   
                   
                   
                 CCCTCCAT 
               
               
                   
               
               
                 189 
                 FTHL16 
                 XR_041433.1 
                   255- 
                 GGACTCAGAGGCCGCCATCAAC 
               
               
                   
                   
                   
                   354 
                 CGCCAGATCAACCTAGAGCTCTG 
               
               
                   
                   
                   
                   
                 TGCCTCCTACGTTTACCTGTCCA 
               
               
                   
                   
                   
                   
                 TGTCTTACTGCTTTGACCGTGAT 
               
               
                   
                   
                   
                   
                 GATGTGGCT 
               
               
                   
               
               
                 190 
                 GATA2 
                 NM_00114566 
                  2573- 
                 GTCCAGTTGATTGTACGTAGCCA 
               
               
                   
                   
                 2.1 
                  2672 
                 CAGGAGCCCTGCTATGAAAGGA 
               
               
                   
                   
                   
                   
                 ATAAAACCTACACACAAGGTTG 
               
               
                   
                   
                   
                   
                 GAGCTTTGCAATTCTTTTTGGAA 
               
               
                   
                   
                   
                   
                 AAGAGCTGGG 
               
               
                   
               
               
                 191 
                 GLIS3 
                 NM_00104241 
                   548- 
                 ACTCGCGCTGGCCGGCCGGGGG 
               
               
                   
                   
                 3.1 
                   647 
                 AAGGGACCCGCACGCCGGGCTTT 
               
               
                   
                   
                   
                   
                 GTTGTGGAAATCCCGGTTACCTG 
               
               
                   
                   
                   
                   
                 GCTTATAACCCACACCATGGATA 
               
               
                   
                   
                   
                   
                 ACTTATTGG 
               
               
                   
               
               
                 192 
                 GLRX 
                 ILMN_173730 
                   119- 
                 AAAGCATAGTTGGTCTTGGTGTC 
               
               
                   
                   
                 8.1 
                   218 
                 ATATGGATCAGAGGCACAAGTG 
               
               
                   
                   
                   
                   
                 CAGAGGCTGTGGTCATGCGGAA 
               
               
                   
                   
                   
                   
                 CACTCTGTTATTTAAGATGGCTA 
               
               
                   
                   
                   
                   
                 TCCAGATAAT 
               
               
                   
               
               
                 193 
                 GNL3 
                 NM_014366.4 
                  1733- 
                 TACAGCAGGTGAACAGTCTACA 
               
               
                   
                   
                   
                  1832 
                 AGGTCTTTTATCTTGGATAAAAT 
               
               
                   
                   
                   
                   
                 CATTGAAGAGGATGATGCTTATG 
               
               
                   
                   
                   
                   
                 ACTTCAGTACAGATTATGTGTAA 
               
               
                   
                   
                   
                   
                 CAGAACAAT 
               
               
                   
               
               
                 194 
                 GNS 
                 NM_002076.3 
                  4988- 
                 CCTGTGTTTGCATCCTCTGTTCC 
               
               
                   
                   
                   
                  5087 
                 TATTCTGCCCTTGCTCTGTGTCA 
               
               
                   
                   
                   
                   
                 TCTCAGTCATTTGACTTAGAAAG 
               
               
                   
                   
                   
                   
                 TGCCCTTCAAAAGGACCCTGTTC 
               
               
                   
                   
                   
                   
                 ACTGCTGC 
               
               
                   
               
               
                 195 
                 GOLGA3 
                 NM_005895.3 
                  8961- 
                 CTCACTGACCGGAAGGTCCAGGT 
               
               
                   
                   
                   
                  9060 
                 GAATCTCGTCATAAGTGATCTCA 
               
               
                   
                   
                   
                   
                 GGCTCTCACAGGATCCGGAGGG 
               
               
                   
                   
                   
                   
                 AAATGTGTTAGAGGGTCTGGAA 
               
               
                   
                   
                   
                   
                 AATTCAGTGC 
               
               
                   
               
               
                 196 
                 GPATCH 
                 NM_022078.2 
                  1686- 
                 AGTCTGGGAGCAGCAGTCTTCGT 
               
               
                   
                 3 
                   
                  1785 
                 GGCTGGTTCAGGGTGTTTTGTTC 
               
               
                   
                   
                   
                   
                 CGAGCCTGCCTGCCTGCCGGTTC 
               
               
                   
                   
                   
                   
                 TATACCTCAGGGGCATTTTTACA 
               
               
                   
                   
                   
                   
                 AAAAGCCC 
               
               
                   
               
               
                 197 
                 GPI 
                 NM_000175.2 
                  1696- 
                 CAGTGCTCAAGTGACCTCTCACG 
               
               
                   
                   
                   
                  1795 
                 ACGCTTCTACCAATGGGCTCATC 
               
               
                   
                   
                   
                   
                 AACTTCATCAAGCAGCAGCGCG 
               
               
                   
                   
                   
                   
                 AGGCCAGAGTCCAATAAACTCGT 
               
               
                   
                   
                   
                   
                 GCTCATCTG 
               
               
                   
               
               
                 198 
                 GPR65 
                 NM_003608.3 
                  1899- 
                 TATGATTTTTCTCACTCTTTCTT 
               
               
                   
                   
                   
                  1998 
                 TGGACTCCAGGGTGTCAGCCATC 
               
               
                   
                   
                   
                   
                 AGGTCTCCTAATTTTGTGTACCG 
               
               
                   
                   
                   
                   
                 GTCTCCAACAACCCCAGCTACTG 
               
               
                   
                   
                   
                   
                 AATACTGC 
               
               
                   
               
               
                 199 
                 GSTO1 
                 NM_004832.2 
                   897- 
                 AGAGCTCTACTTACAGAACAGCC 
               
               
                   
                   
                   
                   996 
                 CTGAGGCCTGTGACTATGGGCTC 
               
               
                   
                   
                   
                   
                 TGAAGGGGGCAGGAGTCAGCAA 
               
               
                   
                   
                   
                   
                 TAAAGCTATGTCTGATATTTTCC 
               
               
                   
                   
                   
                   
                 TTCACTAAT 
               
               
                   
               
               
                 200 
                 GUSH 
                 NM_000181.3 
                  2032- 
                 GGTATCCCCACTCAGTAGCCAAG 
               
               
                   
                   
                   
                  2131 
                 TCACAATGTTTGGAAAACAGCCT 
               
               
                   
                   
                   
                   
                 GTTTACTTGAGCAAGACTGATAC 
               
               
                   
                   
                   
                   
                 CACCTGCGTGTCCCTTCCTCCCC 
               
               
                   
                   
                   
                   
                 GAGTCAGG 
               
               
                   
               
               
                 201 
                 GZMA 
                 NM_006144.3 
                   636- 
                 GCCTCCGAGGTGGAAGAGACTC 
               
               
                   
                   
                   
                   735 
                 GTGCAATGGAGATTCTGGAAGCC 
               
               
                   
                   
                   
                   
                 CTTTGTTGTGCGAGGGTGTTTTC 
               
               
                   
                   
                   
                   
                 CGAGGGGTCACTTCCTTTGGCCT 
               
               
                   
                   
                   
                   
                 TGAAAATAA 
               
               
                   
               
               
                 202 
                 GZMB 
                 NM_004131.3 
                   541- 
                 ACACTACAAGAGGTGAAGATGA 
               
               
                   
                   
                   
                   640 
                 CAGTGCAGGAAGATCGAAAGTG 
               
               
                   
                   
                   
                   
                 CGAATCTGACTTACGCCATTATT 
               
               
                   
                   
                   
                   
                 ACGACAGTACCATTGAGTTGTGC 
               
               
                   
                   
                   
                   
                 GTGGGGGACC 
               
               
                   
               
               
                 203 
                 GZMH 
                 NM_033423.4 
                   718- 
                 GGCCCCTCGTGTGTAAGGACGTA 
               
               
                   
                   
                   
                   817 
                 GCCCAAGGTATTCTCTCCTATGG 
               
               
                   
                   
                   
                   
                 AAACAAAAAAGGGACACCTCCA 
               
               
                   
                   
                   
                   
                 GGAGTCTACATCAAGGTCTCACA 
               
               
                   
                   
                   
                   
                 CTTCCTGCC 
               
               
                   
               
               
                 204 
                 HAT1 
                 NM_003642.3 
                  1235- 
                 AACCAAATAGAAATAAGCATGC 
               
               
                   
                   
                   
                  1334 
                 AACATGAACAGCTGGAAGAGAG 
               
               
                   
                   
                   
                   
                 TTTTCAGGAACTAGTGGAAGATT 
               
               
                   
                   
                   
                   
                 ACCGGCGTGTTATTGAACGACTT 
               
               
                   
                   
                   
                   
                 GCTCAAGAGT 
               
               
                   
               
               
                 205 
                 HAVCR2 
                 NM_032782.3 
                   956- 
                 TATATGAAGTGGAGGAGCCCAA 
               
               
                   
                   
                   
                  1055 
                 TGAGTATTATTGCTATGTCAGCA 
               
               
                   
                   
                   
                   
                 GCAGGCAGCAACCCTCACAACCT 
               
               
                   
                   
                   
                   
                 TTGGGTTGTCGCTTTGCAATGCC 
               
               
                   
                   
                   
                   
                 ATAGATCCA 
               
               
                   
               
               
                 206 
                 HDAC3 
                 NM_003883.3 
                  1765- 
                 AAGATGAAGAGAGAGAGATTTG 
               
               
                   
                   
                   
                  1864 
                 GAAGGGGCTCTGGCTCCCTAACA 
               
               
                   
                   
                   
                   
                 CCTGAATCCCAGATGATGGGAA 
               
               
                   
                   
                   
                   
                 GTATGTTTTCAAGTGTGGGGAGG 
               
               
                   
                   
                   
                   
                 ATATGAAAAT 
               
               
                   
               
               
                 207 
                 HERC1 
                 NM_003922.3 
                 14664- 
                 CAATCGACATGGACAACTACATG 
               
               
                   
                   
                   
                 14763 
                 CTCTCGAGAAACGTGGACAACG 
               
               
                   
                   
                   
                   
                 CCGAGGGCTCCGACACTGACTAC 
               
               
                   
                   
                   
                   
                 TGACCGTGCGGGTGCTCTCACCC 
               
               
                   
                   
                   
                   
                 TCCCTTCTC 
               
               
                   
               
               
                 208 
                 HERC3 
                 NM_014606.2 
                  3796- 
                 TAAGAATGATTTAGACTGACCTG 
               
               
                   
                   
                   
                  3895 
                 TCCTTTTTTATCTGCGCATGCGA 
               
               
                   
                   
                   
                   
                 GAACATCACCTTCCTCTGTACAC 
               
               
                   
                   
                   
                   
                 TTGGAAATGCCTCTGGCTTGTTG 
               
               
                   
                   
                   
                   
                 CAGCCCTC 
               
               
                   
               
               
                 209 
                 HK3 
                 NM_002115.2 
                  2785- 
                 AGTCAGAGGATGGGTCCGGCAA 
               
               
                   
                   
                   
                  2884 
                 AGGTGCGGCCCTGGTCACCGCTG 
               
               
                   
                   
                   
                   
                 TTGCCTGCCGCCTTGCGCAGTTG 
               
               
                   
                   
                   
                   
                 ACTCGTGTCTGAGGAAACCTCCA 
               
               
                   
                   
                   
                   
                 GGCTGAGGA 
               
               
                   
               
               
                 210 
                 HLA-B 
                 NM_005514.6 
                   938- 
                 CCCTGAGATGGGAGCCGTCTTCC 
               
               
                   
                   
                   
                  1037 
                 CAGTCCACCGTCCCCATCGTGGG 
               
               
                   
                   
                   
                   
                 CATTGTTGCTGGCCTGGCTGTCC 
               
               
                   
                   
                   
                   
                 TAGCAGTTGTGGTCATCGGAGCT 
               
               
                   
                   
                   
                   
                 GTGGTCGC 
               
               
                   
               
               
                 211 
                 HLA- 
                 NM_002118.3 
                    21- 
                 CCCGTGAGCTGGAAGGAACAGA 
               
               
                   
                 DMB 
                   
                   120 
                 TTTAATATCTAGGGGCTGGGTAT 
               
               
                   
                   
                   
                   
                 CCCCACATCACTCATTTGGGGGG 
               
               
                   
                   
                   
                   
                 TCAAGGGACCCGGGCAATATAG 
               
               
                   
                   
                   
                   
                 TATTCTGCTC 
               
               
                   
               
               
                 212 
                 HLA-G 
                 NM_002127.4 
                  1181- 
                 AAGAGCTCAGATTGAAAAGGAG 
               
               
                   
                   
                   
                  1280 
                 GGAGCTACTCTCAGGCTGCAATG 
               
               
                   
                   
                   
                   
                 TGAAACAGCTGCCCTGTGTGGGA 
               
               
                   
                   
                   
                   
                 CTGAGTGGCAAGTCCCTTTGTGA 
               
               
                   
                   
                   
                   
                 CTTCAAGAA 
               
               
                   
               
               
                 213 
                 HMGB1 
                 NM_002128.4 
                   209- 
                 TATGCATTTTTTGTGCAAACTTG 
               
               
                   
                   
                   
                   308 
                 TCGGGAGGAGCATAAGAAGAAGC 
               
               
                   
                   
                   
                   
                 ACCCAGATGCTTCAGTCAACTTC 
               
               
                   
                   
                   
                   
                 TCAGAGTTTTCTAAGAAGTGCTC 
               
               
                   
                   
                   
                   
                 AGAGAGGT 
               
               
                   
               
               
                 214 
                 HMGB2 
                 NM_002129.3 
                   670- 
                 TGCTGCATATCGTGCCAAGGGCA 
               
               
                   
                   
                   
                   769 
                 AAAGTGAAGCAGGAAAGAAGGG 
               
               
                   
                   
                   
                   
                 CCCTGGCAGGCCAACAGGCTCA 
               
               
                   
                   
                   
                   
                 AAGAAGAAGAACGAACCAGAAG 
               
               
                   
                   
                   
                   
                 ATGAGGAGGAG 
               
               
                   
               
               
                 215 
                 HNRNPA 
                 NM_004499.3 
                  1246- 
                 CCCCATGGAAATCACTCTCCTGT 
               
               
                   
                 B 
                   
                  1345 
                 TGACTATTTCCAGAGCTCTAGGT 
               
               
                   
                   
                   
                   
                 GTTTAGGCAGCGTGTGGTGTCTG 
               
               
                   
                   
                   
                   
                 AGAGGCCATAGCGCCATCATGG 
               
               
                   
                   
                   
                   
                 GCTGATTTT 
               
               
                   
               
               
                 216 
                 HNRNPK 
                 NM_031263.2 
                   538- 
                 TCCCTACCTTGGAAGAGGGCCTG 
               
               
                   
                   
                   
                   637 
                 CAGTTGCCATCACCCACTGCAAC 
               
               
                   
                   
                   
                   
                 CAGCCAGCTCCCGCTCGAATCTG 
               
               
                   
                   
                   
                   
                 ATGCTGTGGAATGCTTAAATTAC 
               
               
                   
                   
                   
                   
                 CAACACTA 
               
               
                   
               
               
                 217 
                 HOOK3 
                 NM_032410.3 
                  2391- 
                 GCAAGGTAGAGAAGTTGTGCCG 
               
               
                   
                   
                   
                  2490 
                 CTCAATCACAGACACCTGCACCC 
               
               
                   
                   
                   
                   
                 ACAACATACTTCTGTTACACACA 
               
               
                   
                   
                   
                   
                 AGAACATTTCAGGAAACTCAGCC 
               
               
                   
                   
                   
                   
                 AGCTTATTT 
               
               
                   
               
               
                 218 
                 HOPX 
                 NM_139211.4 
                   590- 
                 AACAATAGGAAGCTATGTGTATC 
               
               
                   
                   
                   
                   689 
                 TTCTGTGTAAAGCAGTGGCTTCA 
               
               
                   
                   
                   
                   
                 CTGGAAAAATGGTGTGGCTAGC 
               
               
                   
                   
                   
                   
                 ATTTCCCTTTGAGTCATGATGAC 
               
               
                   
                   
                   
                   
                 AGATGGTGT 
               
               
                   
               
               
                 219 
                 HPSE 
                 NM_006665.5 
                  3920- 
                 GAGGTTCCTATAATTGTCTCTGA 
               
               
                   
                   
                   
                  4019 
                 GTAACCCTTTGGAATGGAGAGG 
               
               
                   
                   
                   
                   
                 GTGTTGGTCAGTCTACAAACTGA 
               
               
                   
                   
                   
                   
                 ACACTGCAGTTCTGCGCTTTTTA 
               
               
                   
                   
                   
                   
                 CCAGTGAAA 
               
               
                   
               
               
                 220 
                 HSCB 
                 NM_172002.3 
                   343- 
                 TCCACCCAGATTTCTTCAGCCAG 
               
               
                   
                   
                   
                   442 
                 AGGTCTCAGACTGAAAAGGACTT 
               
               
                   
                   
                   
                   
                 CTCAGAGAAGCATTCGACCCTGG 
               
               
                   
                   
                   
                   
                 TGAATGATGCCTATAAGACCCTC 
               
               
                   
                   
                   
                   
                 CTGGCCCC 
               
               
                   
               
               
                 221 
                 HSD11B 
                 NM_181755.1 
                   156- 
                 GCCTACTACTACTATTCTGCAAA 
               
               
                   
                 1 
                   
                   255 
                 CGAGGAATTCAGACCAGAGATG 
               
               
                   
                   
                   
                   
                 CTCCAAGGAAAGAAAGTGATTG 
               
               
                   
                   
                   
                   
                 TCACAGGGGCCAGCAAAGGGAT 
               
               
                   
                   
                   
                   
                 CGGAAGAGAGA 
               
               
                   
               
               
                 222 
                 HSP90 
                 NM_007355.3 
                  1531- 
                 GGCATTCTCTAAAAATCTCAAGC 
               
               
                   
                 AB1 
                   
                  1630 
                 TTGGAATCCACGAAGACTCCACT 
               
               
                   
                   
                   
                   
                 AACCGCCGCCGCCTGTCTGAGCT 
               
               
                   
                   
                   
                   
                 GCTGCGCTATCATACCTCCCAGT 
               
               
                   
                   
                   
                   
                 CTGGAGAT 
               
               
                   
               
               
                 223 
                 HSPA6 
                 NM_002155.4 
                  1990- 
                 GTGGCACTCAAGCCCGCCAGGG 
               
               
                   
                   
                   
                  2089 
                 GGACCCCAGCACCGGCCCCATCA 
               
               
                   
                   
                   
                   
                 TTGAGGAGGTTGATTGAATGGCC 
               
               
                   
                   
                   
                   
                 CTTCGTGATAAGTCAGCTGTGAC 
               
               
                   
                   
                   
                   
                 TGTCAGGGC 
               
               
                   
               
               
                 224 
                 HUWE1 
                 NM_031407.6 
                 13637- 
                 CCACCAACTCACCGTGTGTGTCC 
               
               
                   
                   
                   
                 13736 
                 CAGCTGCCCCATCTTCCCCAGCG 
               
               
                   
                   
                   
                   
                 CATACCTGTTCCTCTTCTCATTC 
               
               
                   
                   
                   
                   
                 TCTCCCCGCCGCCTGTTTCCTCA 
               
               
                   
                   
                   
                   
                 CCTTCTCT 
               
               
                   
               
               
                 225 
                 HVCN1 
                 NM_032369.3 
                   747- 
                 TGTTCCAGGAGCACCAGTTTGAG 
               
               
                   
                   
                   
                   846 
                 GCTCTGGGCCTGCTGATTCTGCT 
               
               
                   
                   
                   
                   
                 CCGGCTGTGGCGGGTGGCCCGG 
               
               
                   
                   
                   
                   
                 ATCATCAATGGGATTATCATCTC 
               
               
                   
                   
                   
                   
                 AGTTAAGAC 
               
               
                   
               
               
                 226 
                 IDO1 
                 NM_002164.3 
                    51- 
                 CTATTATAAGATGCTCTGAAAAC 
               
               
                   
                   
                   
                   150 
                 TCTTCAGACACTGAGGGGCACCA 
               
               
                   
                   
                   
                   
                 GAGGAGCAGACTACAAGAATGG 
               
               
                   
                   
                   
                   
                 CACACGCTATGGAAAACTCCTGG 
               
               
                   
                   
                   
                   
                 ACAATCAGT 
               
               
                   
               
               
                 227 
                 IDS 
                 NM_006123.4 
                  1016- 
                 TGGATGGACATCAGGCAACGGG 
               
               
                   
                   
                   
                  1115 
                 AAGACGTCCAAGCCTTAAACATC 
               
               
                   
                   
                   
                   
                 AGTGTGCCGTATGGTCCAATTCC 
               
               
                   
                   
                   
                   
                 TGTGGACTTTCAGCGGAAAATCC 
               
               
                   
                   
                   
                   
                 GCCAGAGCT 
               
               
                   
               
               
                 228 
                 IER5 
                 NM_016545.4 
                  1802- 
                 ACTTTACACCTACCCCTCACCGG 
               
               
                   
                   
                   
                  1901 
                 AAAGCTAGACCCGCTTCAGGGCC 
               
               
                   
                   
                   
                   
                 AGGAGTGGCGTTTCCGCACAGG 
               
               
                   
                   
                   
                   
                 ATTTCCTAAGACGAGAGGGATTT 
               
               
                   
                   
                   
                   
                 AGCCAAGAG 
               
               
                   
               
               
                 229 
                 IFI27L 
                 NM_032036.2 
                   305- 
                 GTCAGTGTTGGGGGCCTGCTTGG 
               
               
                   
                 2 
                   
                   404 
                 GGAATTCACCTTCTTCTTCTCTC 
               
               
                   
                   
                   
                   
                 CCAGCTGAACCCGAGGCTAAAGA 
               
               
                   
                   
                   
                   
                 AGATGAGGCAAGAGAAAATGTA 
               
               
                   
                   
                   
                   
                 CCCCAAGGT 
               
               
                   
               
               
                 230 
                 IFNA17 
                 NM_021268.2 
                   292- 
                 TGAGATGATCCAGCAGACCTTCA 
               
               
                   
                   
                   
                   391 
                 ATCTCTTCAGCACAGAGGACTCA 
               
               
                   
                   
                   
                   
                 TCTGCTGCTTGGGAACAGAGCCT 
               
               
                   
                   
                   
                   
                 CCTAGAAAAATTTTCCACTGAAC 
               
               
                   
                   
                   
                   
                 TTTACCAG 
               
               
                   
               
               
                 231 
                 IFNAR1 
                 NM_000629.2 
                  3124- 
                 CTAATCAGCTCTCAGTGATCAAC 
               
               
                   
                   
                   
                  3223 
                 CCACTCTTGTTATGGGTGGTCTC 
               
               
                   
                   
                   
                   
                 TGTCACTTTGAATGCCAGGCTGG 
               
               
                   
                   
                   
                   
                 CTTCTCGTCTAGCAGTATTCAGA 
               
               
                   
                   
                   
                   
                 TACCCCTT 
               
               
                   
               
               
                 232 
                 IFNAR2 
                 NM_000874.3 
                   632- 
                 AAATACCACAAGATCATTTTGTG 
               
               
                   
                   
                   
                   731 
                 ACCTCACAGATGAGTGGAGAAG 
               
               
                   
                   
                   
                   
                 CACACACGAGGCCTATGTCACCG 
               
               
                   
                   
                   
                   
                 TCCTAGAAGGATTCAGCGGGAA 
               
               
                   
                   
                   
                   
                 CACAACGTTG 
               
               
                   
               
               
                 233 
                 IFNGR1 
                 NM_000416.1 
                  1141- 
                 CCCGGGCAGCCATCTGACTCCAA 
               
               
                   
                   
                   
                  1240 
                 TAGAGAGAGAGAGTTCTTCACCT 
               
               
                   
                   
                   
                   
                 TTAAGTAGTAACCAGTCTGAACC 
               
               
                   
                   
                   
                   
                 TGGCAGCATCGCTTTAAACTCGT 
               
               
                   
                   
                   
                   
                 ATCACTCC 
               
               
                   
               
               
                 234 
                 IGFBP7 
                 NM_001553.2 
                   584- 
                 ATCGGAATCCCGACACCTGTCCT 
               
               
                   
                   
                   
                   683 
                 CATCTGGAACAAGGTAAAAAGG 
               
               
                   
                   
                   
                   
                 GGTCACTATGGAGTTCAAAGGAC 
               
               
                   
                   
                   
                   
                 AGAACTCCTGCCTGGTGACCGGG 
               
               
                   
                   
                   
                   
                 ACAACCTGG 
               
               
                   
               
               
                 235 
                 IL16 
                 NM_004513.4 
                  1263- 
                 GGCATCTCCAACATCATCATCCA 
               
               
                   
                   
                   
                  1362 
                 ACGAAGACTCAGCTGCAAATGG 
               
               
                   
                   
                   
                   
                 TTCTGCTGAAACATCTGCCTTGG 
               
               
                   
                   
                   
                   
                 ACACAGGGTTCTCGCTCAACCTT 
               
               
                   
                   
                   
                   
                 TCAGAGCTG 
               
               
                   
               
               
                 236 
                 IL1B 
                 NM_000576.2 
                   841- 
                 GGGACCAAAGGCGGCCAGGATA 
               
               
                   
                   
                   
                   940 
                 TAACTGACTTCACCATGCAATTT 
               
               
                   
                   
                   
                   
                 GTGTCTTCCTAAAGAGAGCTGTA 
               
               
                   
                   
                   
                   
                 CCCAGAGAGTCCTGTGCTGAATG 
               
               
                   
                   
                   
                   
                 TGGACTCAA 
               
               
                   
               
               
                 237 
                 IL1R2 
                 NM_173343.1 
                   114- 
                 TGCTTCTGCCACGTGCTGCTGGG 
               
               
                   
                   
                   
                   213 
                 TCTCAGTCCTCCACTTCCCGTGT 
               
               
                   
                   
                   
                   
                 CCTCTGGAAGTTGTCAGGAGCAA 
               
               
                   
                   
                   
                   
                 TGTTGCGCTTGTACGTGTTGGTA 
               
               
                   
                   
                   
                   
                 ATGGGAGT 
               
               
                   
               
               
                 238 
                 IL4 
                 NM_000589.2 
                   626- 
                 GACACTCGCTGCCTGGGTGCGAC 
               
               
                   
                   
                   
                   725 
                 TGCACAGCAGTTCCACAGGCACA 
               
               
                   
                   
                   
                   
                 AGCAGCTGATCCGATTCCTGAAA 
               
               
                   
                   
                   
                   
                 CGGCTCGACAGGAACCTCTGGG 
               
               
                   
                   
                   
                   
                 GCCTGGCGG 
               
               
                   
               
               
                 239 
                 IL7 
                 NM_000880.2 
                    39- 
                 AATAACCCAGCTTGCGTCCTGCA 
               
               
                   
                   
                   
                   138 
                 CACTTGTGGCTTCCGTGCACACA 
               
               
                   
                   
                   
                   
                 TTAACAACTCATGGTTCTAGCTC 
               
               
                   
                   
                   
                   
                 CCAGTCGCCAAGCGTTGCCAAGG 
               
               
                   
                   
                   
                   
                 CGTTGAGA 
               
               
                   
               
               
                 240 
                 INTS4 
                 NM_033547.3 
                   652- 
                 CCCACGTGTCAGAACAGCAGCTA 
               
               
                   
                   
                   
                   751 
                 TAAAAGCCATGTTGCAGCTCCAT 
               
               
                   
                   
                   
                   
                 GAAAGAGGACTGAAATTACACC 
               
               
                   
                   
                   
                   
                 AAACAATTTATAATCAGGCCTGT 
               
               
                   
                   
                   
                   
                 AAATTACTC 
               
               
                   
               
               
                 241 
                 IRAK2 
                 NM_001570.3 
                  1286- 
                 GTGTTGGCCGAGGTCCTCACGGG 
               
               
                   
                   
                   
                  1385 
                 CATCCCTGCAATGGATAACAACC 
               
               
                   
                   
                   
                   
                 GAAGCCCGGTTTACCTGAAGGAC 
               
               
                   
                   
                   
                   
                 TTACTCCTCAGTGATATTCCAAG 
               
               
                   
                   
                   
                   
                 CAGCACCG 
               
               
                   
               
               
                 242 
                 IRF1 
                 NM_002198.1 
                   511- 
                 CTGTGCGAGTGTACCGGATGCTT 
               
               
                   
                   
                   
                   610 
                 CCACCTCTCACCAAGAACCAGAG 
               
               
                   
                   
                   
                   
                 AAAAGAAAGAAAGTCGAAGTCC 
               
               
                   
                   
                   
                   
                 AGCCGAGATGCTAAGAGCAAGG 
               
               
                   
                   
                   
                   
                 CCAAGAGGAA 
               
               
                   
               
               
                 243 
                 IRF4 
                 NM_002460.1 
                   326- 
                 GGGCACTGTTTAAAGGAAAGTTC 
               
               
                   
                   
                   
                   425 
                 CGAGAAGGCATCGACAAGCCGG 
               
               
                   
                   
                   
                   
                 ACCCTCCCACCTGGAAGACGCGC 
               
               
                   
                   
                   
                   
                 CTGCGGTGCGCTTTGAACAAGAG 
               
               
                   
                   
                   
                   
                 CAATGACTT 
               
               
                   
               
               
                 244 
                 KIAA 
                 NM_014761.3 
                  2187- 
                 ATGGATGGGACTCTTATGTCATA 
               
               
                   
                 0174 
                   
                  2286 
                 ACTTCTGTTACTCCTTTGGCCCA 
               
               
                   
                   
                   
                   
                 TAGCTAAGGTCATCCTTCCCCAC 
               
               
                   
                   
                   
                   
                 AGGGGTGGCTTTGGGATTGGATG 
               
               
                   
                   
                   
                   
                 ATACAGCT 
               
               
                   
               
               
                 245 
                 ITCH 
                 NM_00125713 
                   439- 
                 GAGGTGACAAAGAGCCAACAGA 
               
               
                   
                   
                 8.1 
                   538 
                 GACAATAGGAGACTTGTCAATTT 
               
               
                   
                   
                   
                   
                 GTCTTGATGGGCTACAGTTAGAG 
               
               
                   
                   
                   
                   
                 TCTGAAGTTGTTACCAATGGTGA 
               
               
                   
                   
                   
                   
                 AACTACATG 
               
               
                   
               
               
                 246 
                 ITFG2 
                 NM_018463.3 
                  1985- 
                 GTCTGGTCTTACCCATGTTCCTA 
               
               
                   
                   
                   
                  2084 
                 GCAACCCTGAGATGATTTTCTTC 
               
               
                   
                   
                   
                   
                 CATTTACCAAAGCAGCCGGGTCA 
               
               
                   
                   
                   
                   
                 GTGCTTTCTCACGTTGCCGTATT 
               
               
                   
                   
                   
                   
                 CTTCAGGT 
               
               
                   
               
               
                 247 
                 ITGAE 
                 NM_002208.4 
                  3406- 
                 CTGAATGCAGAGAACCACAGAA 
               
               
                   
                   
                   
                  3505 
                 CTAAGATCACTGTCGTCTTCCTG 
               
               
                   
                   
                   
                   
                 AAAGATGAGAAGTACCATTCTTT 
               
               
                   
                   
                   
                   
                 GCCTATCATCATTAAAGGCAGCG 
               
               
                   
                   
                   
                   
                 TTGGTGGAC 
               
               
                   
               
               
                 248 
                 ITGAL 
                 NM_002209.2 
                  3906- 
                 GTGAGGGCTTGTCATTACCAGAC 
               
               
                   
                   
                   
                  4005 
                 GGTTCACCAGCCTCTCTTGGTTT 
               
               
                   
                   
                   
                   
                 CCTTCCTTGGAAGAGAATGTCTG 
               
               
                   
                   
                   
                   
                 ATCTAAATGTGGAGAAACTGTAG 
               
               
                   
                   
                   
                   
                 TCTCAGGA 
               
               
                   
               
               
                 249 
                 JAK1 
                 NM_002227.1 
                   286- 
                 GAGAACACCAAGCTCTGGTAGC 
               
               
                   
                   
                   
                   385 
                 TCCAAATCGCACCATCACCGTTG 
               
               
                   
                   
                   
                   
                 ATGACAAGATGTCCCTCCGGCTC 
               
               
                   
                   
                   
                   
                 CACTACCGGATGAGGTTCTATTT 
               
               
                   
                   
                   
                   
                 CACCAATT 
               
               
                   
               
               
                 250 
                 KIAA 
                 NM_015443.3 
                  4402- 
                 CCTTCACATCCAGATCCCTGTCG 
               
               
                   
                 1267 
                   
                  4501 
                 GTGTTAGTTCCACTCTTGGTCTT 
               
               
                   
                   
                   
                   
                 TCACGCTCCCCTTGCCTGTGGAA 
               
               
                   
                   
                   
                   
                 CATTGTCTGGTCCTAGCTGTGGT 
               
               
                   
                   
                   
                   
                 TCCCATTG 
               
               
                   
               
               
                 251 
                 MYST4 
                 NM_012330.3 
                  6541- 
                 CCCAGACTGTAGCCATGCAGGGT 
               
               
                   
                   
                   
                  6640 
                 CCTGCACGGACTTTAACGATGCA 
               
               
                   
                   
                   
                   
                 AAGAGGCATGAACATGAGTGTG 
               
               
                   
                   
                   
                   
                 AACCTGATGCCAGCGCCAGCCTA 
               
               
                   
                   
                   
                   
                 CAATGTCAA 
               
               
                   
               
               
                 252 
                 KCTD12 
                 NM_138444.3 
                  4208- 
                 ACAAGTAAAATAACTTGACATG 
               
               
                   
                   
                   
                  4307 
                 AGCACCTTTAGATCCCTTCCCCT 
               
               
                   
                   
                   
                   
                 CCATGGGCTTTGGGCCACAGAAT 
               
               
                   
                   
                   
                   
                 GAACCTTTGAGGCCTGTAAAGTG 
               
               
                   
                   
                   
                   
                 GATTGTAAT 
               
               
                   
               
               
                 253 
                 KIAA 
                 NM_014736.4 
                   236- 
                 CGACATCAGTTTCATCGAGGAAA 
               
               
                   
                 0101 
                   
                   335 
                 GCTGAAAATAAATATGCAGGAG 
               
               
                   
                   
                   
                   
                 GGAACCCCGTTTGCGTGCGCCCA 
               
               
                   
                   
                   
                   
                 ACTCCCAAGTGGCAAAAAGGAA 
               
               
                   
                   
                   
                   
                 TTGGAGAATT 
               
               
                   
               
               
                 254 
                 SETD1B 
                 XM_ 
                  7779- 
                 ATCGTGCCCAGTGTTAACCTCGG 
               
               
                   
                   
                 037523.11 
                  7878 
                 CTGGCCTTCACTAAGGGGACTAG 
               
               
                   
                   
                   
                   
                 ACCTCCCTCTCCCCAGGAGCCCC 
               
               
                   
                   
                   
                   
                 AGCCCCAGAGTGGTTTGCAATAA 
               
               
                   
                   
                   
                   
                 TCAAGATA 
               
               
                   
               
               
                 255 
                 KIR2DL 
                 XM_00112635 
                   265- 
                 GAGGTGACATATGCACAGTTGG 
               
               
                   
                 5A 
                 4.1 
                   364 
                 ATCACTGCGTTTTCACACAGACA 
               
               
                   
                   
                   
                   
                 AAAATCACTTCCCCTTCTCAGAG 
               
               
                   
                   
                   
                   
                 GCCCAAGACACCTCCAACAGAT 
               
               
                   
                   
                   
                   
                 ACCACCATGT 
               
               
                   
               
               
                 256 
                 KIR_ 
                 NM_014512.1 
                   719- 
                 TCCGAAACCGGTAACCCCAGAC 
               
               
                   
                 Acti- 
                   
                   818 
                 ACCTACATGTTCTGATTGGGACC 
               
               
                   
                 vat- 
                   
                   
                 TCAGTGGTCAAAATCCCTTTCAC 
               
               
                   
                 ing_ 
                   
                   
                 CATCCTCCTCTTCTTTCTCCTTC 
               
               
                   
                 Sub- 
                   
                   
                 ATCGCTGGT 
               
               
                   
                 group_ 
                   
                   
                   
               
               
                   
                 2 
                   
                   
                   
               
               
                   
               
               
                 257 
                 KIR2D  
                 NM_012313.1 
                     1- 
                 CCGGCAGCACCATGTCGCTCATG 
               
               
                   
                 S3 
                   
                   100 
                 GTCATCAGCATGGCATGTGTTGG 
               
               
                   
                   
                   
                   
                 GTTCTTCTGGCTGCAGGGGGCCT 
               
               
                   
                   
                   
                   
                 GGCCACATGAGGGATTCCGCAG 
               
               
                   
                   
                   
                   
                 AAAACCTTC 
               
               
                   
               
               
                 258 
                 KLRB1 
                 NM_002258.2 
                   357- 
                 CAGCAACTCCGAGAGAAATGCTT 
               
               
                   
                   
                   
                   456 
                 GTTATTTTCTCACACTGTCAACC 
               
               
                   
                   
                   
                   
                 CTTGGAATAACAGTCTAGCTGAT 
               
               
                   
                   
                   
                   
                 TGTTCCACCAAAGAATCCAGCCT 
               
               
                   
                   
                   
                   
                 GCTGCTTA 
               
               
                   
               
               
                 259 
                 KLRC1 
                 NM_002259.3 
                   336- 
                 ACCTATCACTGCAAAGATTTACC 
               
               
                   
                   
                   
                   435 
                 ATCAGCTCCAGAGAAGCTCATTG 
               
               
                   
                   
                   
                   
                 TTGGGATCCTGGGAATTATCTGT 
               
               
                   
                   
                   
                   
                 CTTATCTTAATGGCCTCTGTGGT 
               
               
                   
                   
                   
                   
                 AACGATAG 
               
               
                   
               
               
                 260 
                 KLRC2 
                 NM_002260.3 
                   943- 
                 TATGTGAGTCAGCTTATAGGAAG 
               
               
                   
                   
                   
                  1042 
                 TACCAAGAACAGTCAAACCCAT 
               
               
                   
                   
                   
                   
                 GGAGACAGAAAGTAGAATAGTG 
               
               
                   
                   
                   
                   
                 GTTGCCAATGTCTCAGGGAGGTT 
               
               
                   
                   
                   
                   
                 GAAATAGGAG 
               
               
                   
               
               
                 261 
                 KLRD1 
                 NM_002262.3 
                   597- 
                 CAATTTTACTGGATTGGACTCTC 
               
               
                   
                   
                   
                   696 
                 TTACAGTGAGGAGCACACCGCCT 
               
               
                   
                   
                   
                   
                 GGTTGTGGGAGAATGGCTCTGCA 
               
               
                   
                   
                   
                   
                 CTCTCCCAGTATCTATTTCCATC 
               
               
                   
                   
                   
                   
                 ATTTGAAA 
               
               
                   
               
               
                 262 
                 KLRF1 
                 NM_016523.1 
                   544- 
                 TATACAGAAAAACCTAAGACAA 
               
               
                   
                   
                   
                   643 
                 TTAAACTACGTATGGATTGGGCT 
               
               
                   
                   
                   
                   
                 TAACTTTACCTCCTTGAAAATGA 
               
               
                   
                   
                   
                   
                 CATGGACTTGGGTGGATGGTTCT 
               
               
                   
                   
                   
                   
                 CCAATAGAT 
               
               
                   
               
               
                 263 
                 KLRF1  
                 NM_016523.2 
                   849- 
                 AAGTGCAATTAAATGCCAAAATC 
               
               
                   
                 b 
                   
                   948 
                 TCTTCTCCCTTCTCCCTCCATCA 
               
               
                   
                   
                   
                   
                 TCGACACTGGTCTAGCCTCAGAG 
               
               
                   
                   
                   
                   
                 TAACCCCTGTTAACAAACTAAAA 
               
               
                   
                   
                   
                   
                 TGTACACT 
               
               
                   
               
               
                 264 
                 KRTAP 
                 NM_198696.2 
                   213- 
                 CTGCTGCCAGGCGGCCTGTGAGC 
               
               
                   
                 10-3 
                   
                   312 
                 CCAGCCCCTGCCAGTCAGGCTGC 
               
               
                   
                   
                   
                   
                 ACCAGCTCCTGCACGCCCTCGTG 
               
               
                   
                   
                   
                   
                 CTGCCAGCAGTCTAGCTGCCAGC 
               
               
                   
                   
                   
                   
                 CAGCTTGC 
               
               
                   
               
               
                 265 
                 KYNU 
                 NM_00103299 
                   936- 
                 TTGCCTGCTGGTGTTCCTACAAG 
               
               
                   
                   
                 8.1 
                  1035 
                 TATTTAAATGCAGGAGCAGGAG 
               
               
                   
                   
                   
                   
                 GAATTGCTGGTGCCTTCATTCAT 
               
               
                   
                   
                   
                   
                 GAAAAGCATGCCCATACGATTA 
               
               
                   
                   
                   
                   
                 AACCTGCGAG 
               
               
                   
               
               
                 266 
                 LAMA5 
                 NM_005560.4 
                 11163- 
                 CCAACCCCGGCCCCTGGTCAGGC 
               
               
                   
                   
                   
                 11262 
                 CCCTGCAGCTGCCTCACACCGCC 
               
               
                   
                   
                   
                   
                 CCTTGTGCTCGCCTCATAGGTGT 
               
               
                   
                   
                   
                   
                 CTATTTGGACTCTAAGCTCTACG 
               
               
                   
                   
                   
                   
                 GGTGACAG 
               
               
                   
               
               
                 267 
                 LDHA 
                 NM_00116541 
                  1348- 
                 ATCTTGTGTAGTCTTCAACTGGT 
               
               
                   
                   
                 6.1 
                  1447 
                 TAGTGTGAAATAGTTCTGCCACC 
               
               
                   
                   
                   
                   
                 TCTGACGCACCACTGCCAATGCT 
               
               
                   
                   
                   
                   
                 GTACGTACTGCATTTGCCCCTTG 
               
               
                   
                   
                   
                   
                 AGCCAGGT 
               
               
                   
               
               
                 268 
                 LEF1 
                 NM_016269.4 
                  3136- 
                 AACACATAGTGGCTTCTCCGCCC 
               
               
                   
                   
                   
                  3235 
                 TTGTAAGGTGTTCAGTAGAGCTA 
               
               
                   
                   
                   
                   
                 AATAAATGTAATAGCCAAACCC 
               
               
                   
                   
                   
                   
                 ACTCTGTTGGTAGCAATTGGCAG 
               
               
                   
                   
                   
                   
                 CCCTATTTC 
               
               
                   
               
               
                 269 
                 LETM2 
                 NM_144652.3 
                  1331- 
                 AAAGGACCCATCACTTCTTCTGA 
               
               
                   
                   
                   
                  1430 
                 AGAACCTACACTCCAGGCCAAAT 
               
               
                   
                   
                   
                   
                 CACAAATGACGGCCCAGAACAG 
               
               
                   
                   
                   
                   
                 CAAGGCTAGTTCAAAAGGAGCA 
               
               
                   
                   
                   
                   
                 TAAAGGACTA 
               
               
                   
               
               
                 270 
                 LIF 
                 NM_002309.3 
                  1241- 
                 GGGATGGAAGGCTGTCTTCTTTT 
               
               
                   
                   
                   
                  1340 
                 GAGGATGATCAGAGAACTTGGG 
               
               
                   
                   
                   
                   
                 CATAGGAACAATCTGGCAGAAG 
               
               
                   
                   
                   
                   
                 TTTCCAGAAGGAGGTCACTTGGC 
               
               
                   
                   
                   
                   
                 ATTCAGGCTC 
               
               
                   
               
               
                 271 
                 LILRA5 
                 NM_021250.3 
                  1044- 
                 TTGAATGCTGGAGCCTTGGAAGC 
               
               
                   
                   
                   
                  1143 
                 GAATCTGATGGTCCTAGGAGGTT 
               
               
                   
                   
                   
                   
                 CGGGAAGACCATCTGAGGCCTAT 
               
               
                   
                   
                   
                   
                 GCCATCTGGACTGTCTGCTGGCA 
               
               
                   
                   
                   
                   
                 ATTTCTTT 
               
               
                   
               
               
                 272 
                 LILRA5  
                 NM_181879.2 
                   546- 
                 CACCCTCTCAGCCCTGCCCAGTC 
               
               
                   
                 b 
                   
                   645 
                 CTGTGGTGACCTCAGGAGAGAA 
               
               
                   
                   
                   
                   
                 CGTGACCCTCCAGTGTGGCTCAC 
               
               
                   
                   
                   
                   
                 GGCTGAGATTCGACAGGTTCATT 
               
               
                   
                   
                   
                   
                 CTGACTGAG 
               
               
                   
               
               
                 273 
                 LOC 
                 NR_002809.2 
                   471- 
                 GCGGCAGCCAATCAGCGCGCGG 
               
               
                   
                 338799 
                   
                   570 
                 CTTCTATAGGGCTTGAGTTATTA 
               
               
                   
                   
                   
                   
                 GACGCTGATCTCAAAACATCCTT 
               
               
                   
                   
                   
                   
                 CATCAGACACGAAGGAGAGGCC 
               
               
                   
                   
                   
                   
                 AACAGATGAG 
               
               
                   
               
               
                 274 
                 LOC100 
                 XM_00171659 
                   568- 
                 AGGGTCATGCAGCTACTGAGGTC 
               
               
                   
                 129022 
                 1.1 
                   667 
                 ACAGCCTGGATTCATACACAGGT 
               
               
                   
                   
                   
                   
                 CTGACTCCTGAGCACTTAGCCAG 
               
               
                   
                   
                   
                   
                 GTGGCTGTAACAGTGTTCCCAGA 
               
               
                   
                   
                   
                   
                 AACACAGG 
               
               
                   
               
               
                 275 
                 LOC100 
                 XM_00173282 
                  1148- 
                 ACCTGTCTTCCGGGTCTGTTCAC 
               
               
                   
                 129697 
                 2.2 
                  1247 
                 CCGTCCCCTGGACTGGCACCAGC 
               
               
                   
                   
                   
                   
                 ACAGAGGGTCGAGTGTTGGCAC 
               
               
                   
                   
                   
                   
                 CTGTCTTCTGGGTCTCCATCCCT 
               
               
                   
                   
                   
                   
                 CCCTTTGTT 
               
               
                   
               
               
                 276 
                 LOC100 
                 XM_00171715 
                  1469- 
                 GAGAATGTCTGCGCGGAGACAG 
               
               
                   
                 130229 
                 8.1 
                  1568 
                 CATAGCTCTGTAGAAATGAGTGG 
               
               
                   
                   
                   
                   
                 CAGCGTATGTAACCTGGCATTTT 
               
               
                   
                   
                   
                   
                 GAACCCAGGAGCACAATTTTATT 
               
               
                   
                   
                   
                   
                 AAAGGAAAA 
               
               
                   
               
               
                 277 
                 LOC100 
                 XR_036994.1 
                    15- 
                 GAGTAGTAGGTGGACAGCCGTC 
               
               
                   
                 132797 
                   
                   114 
                 CCACACAAGGGTTTGTATCTGGG 
               
               
                   
                   
                   
                   
                 CTACACAGATTCCCTTCAGAAAA 
               
               
                   
                   
                   
                   
                 GCACCAATGTAAGCAACTCCCTT 
               
               
                   
                   
                   
                   
                 ACAGTTGCT 
               
               
                   
               
               
                 278 
                 LOC100 
                 XR_039238.1 
                   342- 
                 GAGATAGCTTCCTGAAATGTGTG 
               
               
                   
                 133273 
                   
                   441 
                 AAGGAAAATGATCAGAAAAAGA 
               
               
                   
                   
                   
                   
                 AAGAAGCCAAAGAGAAAGGTAC 
               
               
                   
                   
                   
                   
                 CTGGGTTCAACTAAAGTGCCAGC 
               
               
                   
                   
                   
                   
                 CTGCTCCACC 
               
               
                   
               
               
                 279 
                 LOC 
                 NM_144692.1 
                  3367- 
                 GCTCTGTCCTTTGCCGCTCAGAC 
               
               
                   
                 148137 
                   
                  3466 
                 CAAAAACCTTAGAGCTGTCTTTG 
               
               
                   
                   
                   
                   
                 ACTTCTGTCTTTCCCTTCCACCC 
               
               
                   
                   
                   
                   
                 ACAGTTAACCAGGAAATCCTGCC 
               
               
                   
                   
                   
                   
                 ATCTCCGC 
               
               
                   
               
               
                 280 
                 LOC 
                 NR_024275.1 
                  5062- 
                 GGTTACAGCCATTTTGTGTGATT 
               
               
                   
                 151162 
                   
                  5161 
                 CACTTCGGGGGTTAAGTAATGCA 
               
               
                   
                   
                   
                   
                 GGATTCTGCAAACAAGGTGTCGC 
               
               
                   
                   
                   
                   
                 CGTCCAAATGTACTGTCCTGGCA 
               
               
                   
                   
                   
                   
                 TAGAGAGC 
               
               
                   
               
               
                 281 
                 C1orf 
                 NM_00100380 
                  2561- 
                 ACATGGCGCCACGGCCACTTCCT 
               
               
                   
                 222 
                 8.1 
                  2660 
                 GCTGCCCTGGACCCCGCAAGCCC 
               
               
                   
                   
                   
                   
                 AGGGACATCCAAGAGCACCCCT 
               
               
                   
                   
                   
                   
                 CCTGAGACCCCAGACTCAGAAG 
               
               
                   
                   
                   
                   
                 CAGCGAGAAG 
               
               
                   
               
               
                 282 
                 LOC 
                 XM_934917.1 
                   376- 
                 CCCCTGGTGGACCGCGACCTCCG 
               
               
                   
                 339674 
                   
                   475 
                 CAAGACGCTAATGGTGCGCGAC 
               
               
                   
                   
                   
                   
                 AACCTGGCCTTCGGCGGCCCGGA 
               
               
                   
                   
                   
                   
                 GGTCTGAGCCGACTTGCAAAGG 
               
               
                   
                   
                   
                   
                 GGATAGGCGG 
               
               
                   
               
               
                 283 
                 LOC 
                 XM_371757.4 
                   210- 
                 GCAAAGCACTATCACAAGGAAT 
               
               
                   
                 648000 
                   
                   309 
                 ATAGGCAAATGTACAGAACTGA 
               
               
                   
                   
                   
                   
                 AATTCGAGTGGCGAGGATGGCA 
               
               
                   
                   
                   
                   
                 AGAAAAGCTGGCAACTTCTATGT 
               
               
                   
                   
                   
                   
                 ACCTGCAGAAC 
               
               
                   
               
               
                 284 
                 LOC 
                 XR_017684.2 
                    82- 
                 AAGATTATGTCTTCCCCTGTTTC 
               
               
                   
                 391126 
                   
                   181 
                 CAAAGAGCTGAGACAGAAGTACA 
               
               
                   
                   
                   
                   
                 ATGTGCAATCCATGCCCATCCGA 
               
               
                   
                   
                   
                   
                 AAGGATGATGAAGTTCAGGTTGT 
               
               
                   
                   
                   
                   
                 ACGAGGGC 
               
               
                   
               
               
                 285 
                 LOC 
                 XM_930634.1 
                  1448- 
                 ATGGGACCCACTCTACTGAGGCT 
               
               
                   
                 399753 
                   
                  1547 
                 TTATGTAGAACTCATAGAGGAAG 
               
               
                   
                   
                   
                   
                 CTGGCTTTGAGGAATGAACTACC 
               
               
                   
                   
                   
                   
                 CTGTGCTTTTCTTAGGACTAAAA 
               
               
                   
                   
                   
                   
                 TCTCAGGA 
               
               
                   
               
               
                 286 
                 LOC 
                 XM_934471.1 
                    21- 
                 GACGGTAACCGGGACCCAGTGT 
               
               
                   
                 399942 
                   
                   120 
                 CTGCTCCTGTCACCTTCGCCTCC 
               
               
                   
                   
                   
                   
                 TAATCCCTAGCCACTATGCGAGA 
               
               
                   
                   
                   
                   
                 TGACTCCTTCAACACCTTCAGTG 
               
               
                   
                   
                   
                   
                 AGACGGGTG 
               
               
                   
               
               
                 287 
                 LOC 
                 XM_498648.3 
                   552- 
                 GAGTTTTCCAAACCCTGGATTTC 
               
               
                   
                 440389 
                   
                   651 
                 CTTCGGAGAGAGCTAGATTCTAT 
               
               
                   
                   
                   
                   
                 TCCATTCTTGGAATTCAGCTCCT 
               
               
                   
                   
                   
                   
                 TGCCCTTCTCTGTGACCCCGGAT 
               
               
                   
                   
                   
                   
                 CGCGAATG 
               
               
                   
               
               
                 288 
                 LOC 
                 XM_942885.1 
                  1533- 
                 TGTTGCAAAAGCCAACTACCACT 
               
               
                   
                 440928 
                   
                  1632 
                 GTCAAACTTAGCCCGTTTACAAC 
               
               
                   
                   
                   
                   
                 ATGGGGAAAGGCGTATTTCTTAC 
               
               
                   
                   
                   
                   
                 TAATATCTCAACAACGATAACAA 
               
               
                   
                   
                   
                   
                 TGCTGTAT 
               
               
                   
               
               
                 289 
                 LOC 
                 XR_018937.2 
                   287- 
                 CGGGTGCAGCGGGAAAAGGCTA 
               
               
                   
                 441073 
                   
                   386 
                 ATGGCACAACTGTCCACGTAGGC 
               
               
                   
                   
                   
                   
                 ATTCACCCCAGCAAGGTGGTTAT 
               
               
                   
                   
                   
                   
                 CACTAGGCTAAAACTGGACAAA 
               
               
                   
                   
                   
                   
                 GACTGTGAAA 
               
               
                   
               
               
                 290 
                 LOC 
                 XR_036892.1 
                   591- 
                 GGTGAAGAATTTGTTCTATTATG 
               
               
                   
                 642812 
                   
                   690 
                 AAGATACTGTCTGGGCTAAAAA 
               
               
                   
                   
                   
                   
                 GCTTACAGTGAGTGGAAGATAG 
               
               
                   
                   
                   
                   
                 CAACTTGTAGGGTTGGTGGCTGA 
               
               
                   
                   
                   
                   
                 ACAGGCCGAC 
               
               
                   
               
               
                 291 
                 LOC 
                 XM_927980.1 
                   255- 
                 CTGGCTCAAGGATGGCACGGTGT 
               
               
                   
                 643319 
                   
                   354 
                 TATGTGAGCTCAATAATGCACTG 
               
               
                   
                   
                   
                   
                 TACCCCAAGGGGCAGGTCCCAGT 
               
               
                   
                   
                   
                   
                 AAAGAAGATCCAGGCCTCCACC 
               
               
                   
                   
                   
                   
                 ATGGCCTTC 
               
               
                   
               
               
                 292 
                 LOC 
                 XR_017529.2 
                    38- 
                 CAGGCGCTGCAAGTTCTCCCAGG 
               
               
                   
                 644315 
                   
                   137 
                 AGAAAGCCATGTTCAGTTCGAGC 
               
               
                   
                   
                   
                   
                 GCCAAGATCGTGAAGCCCAATG 
               
               
                   
                   
                   
                   
                 GCGAGAAGCCGGACGAGTTCGA 
               
               
                   
                   
                   
                   
                 GTCCGGCCAT 
               
               
                   
               
               
                 293 
                 LOC 
                 XM_928884.1 
                    13- 
                 GAAGCACTGGTAAATGTCTGCTG 
               
               
                   
                 645914 
                   
                   112 
                 CATTAACTCACTCAGACCAAACT 
               
               
                   
                   
                   
                   
                 TTCTCTTATCTAGGTCCAAAAGG 
               
               
                   
                   
                   
                   
                 AAGCTGCTCGGCTGGAAGGAAC 
               
               
                   
                   
                   
                   
                 CTGGTGAGG 
               
               
                   
               
               
                 294 
                 LOC 
                 XR_018104.1 
                   670- 
                 AGGTGCTGCAAAATTACCAGGA 
               
               
                   
                 647340 
                   
                   769 
                 ATACAGTCTGGCCAACAGCATCT 
               
               
                   
                   
                   
                   
                 ACTACTCTCTGAAGGAGTCCACC 
               
               
                   
                   
                   
                   
                 ACTAGTGAGCAGAGTGCCAGGA 
               
               
                   
                   
                   
                   
                 TGACAGCCAT 
               
               
                   
               
               
                 295 
                 LOC 
                 XR_038906.2 
                  1638- 
                 TGGAGAGAAGAATGAAGAGGTG 
               
               
                   
                 648927 
                   
                  1737 
                 GTGGTTCTGGGTTTGATTTGAGT 
               
               
                   
                   
                   
                   
                 TCACCTGTGGGCAGTGGGCAGTG 
               
               
                   
                   
                   
                   
                 TCTTGGTGAAAGGGAGCGGATA 
               
               
                   
                   
                   
                   
                 CTACTTTTTG 
               
               
                   
               
               
                 296 
                 LOC 
                 XM_938755.1 
                    38- 
                 GCCCTTCTGCCATCAACGAGGTG 
               
               
                   
                 653773 
                   
                   137 
                 GTGACCCAAGAACATACCATCA 
               
               
                   
                   
                   
                   
                 ACATTCACAAGCGCATCCATGGA 
               
               
                   
                   
                   
                   
                 GAGGGCTTCAAGAAGCGTGCTCC 
               
               
                   
                   
                   
                   
                 TCGGGCACT 
               
               
                   
               
               
                 297 
                 LOC 
                 XR_015610.3 
                  1861- 
                 GTAGTTGTCCACTGCTTTCCTGG 
               
               
                   
                 728533 
                   
                  1960 
                 ATGGATGGGACTCTTATGTCATA 
               
               
                   
                   
                   
                   
                 ACTTCTATACTCCTTTGGCCCAT 
               
               
                   
                   
                   
                   
                 AGCTAAGGTCATCCTTCCCCACA 
               
               
                   
                   
                   
                   
                 GGGGTGGC 
               
               
                   
               
               
                 298 
                 LOC 
                 XM_00113319 
                   510- 
                 CCAAACCAAAAGAGGCAAGCAA 
               
               
                   
                 728835 
                 0.1 
                   609 
                 GTCTGCGCTGACCCCAGTGAGTC 
               
               
                   
                   
                   
                   
                 CTGGGTCCAGGAGTACGTGTATG 
               
               
                   
                   
                   
                   
                 ACCTGGAACTGAACTGAGCTGCT 
               
               
                   
                   
                   
                   
                 CAGAGACAG 
               
               
                   
               
               
                 299 
                 LOC 
                 XR_040891.2 
                   625- 
                 CCCTGGGTGCCCCTTAACCCGGG 
               
               
                   
                 729887 
                   
                   724 
                 CGGTAGCTCGTTAAGATGGCGAA 
               
               
                   
                   
                   
                   
                 GTGTCCGGTCCGGAACACGCGA 
               
               
                   
                   
                   
                   
                 AACCCCAAATCCCGCCTGCCCGA 
               
               
                   
                   
                   
                   
                 CCTCCTGAC 
               
               
                   
               
               
                 300 
                 LOC 
                 XM_00113427 
                   765- 
                 GCGCGGTTGCGGTTAGCGGGCGC 
               
               
                   
                 732111 
                 5.1 
                   864 
                 GGTGCCAAAGCTGCCATCCCCAG 
               
               
                   
                   
                   
                   
                 CTCACAGCTCCTCATATCCACCC 
               
               
                   
                   
                   
                   
                 TGCCCTCATCTTTATGAATTGCG 
               
               
                   
                   
                   
                   
                 TGTAGACC 
               
               
                   
               
               
                 301 
                 LOC 
                 XM_00113301 
                   182- 
                 GCCCTTCAGAGCTGCGGGAGATC 
               
               
                   
                 732371 
                 9.1 
                   281 
                 ATTGATGAGTGCCGGGCCCATGA 
               
               
                   
                   
                   
                   
                 TCCCTCTGTGCGGCCCTCTGTGG 
               
               
                   
                   
                   
                   
                 ATGAGCAGAAGCGCAGACTTAA 
               
               
                   
                   
                   
                   
                 TGATGTGTT 
               
               
                   
               
               
                 302 
                 LOC 
                 NM_00109977 
                  2666- 
                 ATGTTGCATTGACTAGAGGAAAG 
               
               
                   
                 91431 
                 6.1 
                  2765 
                 AGGCATTTGTTGATTGTGGGAAA 
               
               
                   
                   
                   
                   
                 TTTAGCCTGTTTGAGGAAAAATC 
               
               
                   
                   
                   
                   
                 AACTTTGGGGACGAGTGATCCAA 
               
               
                   
                   
                   
                   
                 CACTGCGA 
               
               
                   
               
               
                 303 
                 P2RY5 
                 NM_005767.5 
                  2026- 
                 AGATTGTTTGCACTGGCGTGTGG 
               
               
                   
                   
                   
                  2125 
                 TTAACTGTGATCGGAGGAAGTGC 
               
               
                   
                   
                   
                   
                 ACCCGCCGTTTTTGTTCAGTCTA 
               
               
                   
                   
                   
                   
                 CCCACTCTCAGGGTAACAATGCC 
               
               
                   
                   
                   
                   
                 TCAGAAGC 
               
               
                   
               
               
                 304 
                 LPCAT4 
                 NM_153613.2 
                  1560- 
                 CCCCACACACCTCTCGAGGCACC 
               
               
                   
                   
                   
                  1659 
                 TCCCAGACACCAAATGCCTCATC 
               
               
                   
                   
                   
                   
                 CCCAGGCAACCCCACTGCTCTGG 
               
               
                   
                   
                   
                   
                 CCAATGGGACTGTGCAAGCACCC 
               
               
                   
                   
                   
                   
                 AAGCAGAA 
               
               
                   
               
               
                 305 
                 LPIN2 
                 NM_014646.2 
                  5620- 
                 AGAAAAAACTTAAAAATGGGAT 
               
               
                   
                   
                   
                  5719 
                 GTCCTAAAATGAAAGCTGCTCAA 
               
               
                   
                   
                   
                   
                 AGTCACAGAACAACCGAGGGAC 
               
               
                   
                   
                   
                   
                 AAAGGAGATTGGATGACTGGGA 
               
               
                   
                   
                   
                   
                 AGCGCTGGCCC 
               
               
                   
               
               
                 306 
                 C1orf 
                 NM_018372.3 
                  1543- 
                 TTCCAATACCCAGCTTGCTTCCA 
               
               
                   
                 103 
                   
                  1642 
                 TGGCCAATCTAAGGGCAGAGAA 
               
               
                   
                   
                   
                   
                 GAATAAAGTGGAGAAACCATCT 
               
               
                   
                   
                   
                   
                 CCTTCTACCACAAATCCACATAT 
               
               
                   
                   
                   
                   
                 GAACCAATCC 
               
               
                   
               
               
                 307 
                 LRRC47 
                 NM_020710.2 
                  2461- 
                 GGGTCAGTGACGGACACTTACCT 
               
               
                   
                   
                   
                  2560 
                 GACAGCGGATCCACAATATTCTC 
               
               
                   
                   
                   
                   
                 GTGCAGTGTGTTTGGAATCCTGG 
               
               
                   
                   
                   
                   
                 TCTGGGCTCTCGTCGTTGGCCTT 
               
               
                   
                   
                   
                   
                 GTAGATCA 
               
               
                   
               
               
                 308 
                 LY96 
                 NM_015364.4 
                   439- 
                 AAGGGAGAGACTGTGAATACAA 
               
               
                   
                   
                   
                   538 
                 CAATATCATTCTCCTTCAAGGGA 
               
               
                   
                   
                   
                   
                 ATAAAATTTTCTAAGGGAAAATA 
               
               
                   
                   
                   
                   
                 CAAATGTGTTGTTGAAGCTATTT 
               
               
                   
                   
                   
                   
                 CTGGGAGCC 
               
               
                   
               
               
                 309 
                 LYN 
                 NM_002350.1 
                  1286- 
                 TCCTGAAGAGCGATGAAGGTGG 
               
               
                   
                   
                   
                  1385 
                 CAAAGTGCTGCTTCCAAAGCTCA 
               
               
                   
                   
                   
                   
                 TTGACTTTTCTGCTCAGATTGCA 
               
               
                   
                   
                   
                   
                 GAGGGAATGGCATACATCGAGC 
               
               
                   
                   
                   
                   
                 GGAAGAACTA 
               
               
                   
               
               
                 310 
                 MAGEA1 
                 NM_004988.4 
                   477- 
                 AGGGGCCAAGCACCTCTTGTATC 
               
               
                   
                   
                   
                   576 
                 CTGGAGTCCTTGTTCCGAGCAGT 
               
               
                   
                   
                   
                   
                 AATCACTAAGAAGGTGGCTGATT 
               
               
                   
                   
                   
                   
                 TGGTTGGTTTTCTGCTCCTCAAA 
               
               
                   
                   
                   
                   
                 TATCGAGC 
               
               
                   
               
               
                 311 
                 MAGEA3 
                 NM_005362.3 
                   850- 
                 ACTGTGCCCCTGAGGAGAAAATC 
               
               
                   
                   
                   
                   949 
                 TGGGAGGAGCTGAGTGTGTTAG 
               
               
                   
                   
                   
                   
                 AGGTGTTTGAGGGGAGGGAAGA 
               
               
                   
                   
                   
                   
                 CAGTATCTTGGGGGATCCCAAGA 
               
               
                   
                   
                   
                   
                 AGCTGCTCAC 
               
               
                   
               
               
                 312 
                 MAP3K7 
                 NM_145333.1 
                   671- 
                 GCCATATTATACTGCTGCCCACG 
               
               
                   
                   
                   
                   770 
                 CAATGAGTTGGTGTTTACAGTGT 
               
               
                   
                   
                   
                   
                 TCCCAAGGAGTGGCTTATCTTCA 
               
               
                   
                   
                   
                   
                 CAGCATGCAACCCAAAGCGCTA 
               
               
                   
                   
                   
                   
                 ATTCACAGG 
               
               
                   
               
               
                 313 
                 MARCKS 
                 NM_002356.6 
                  1800- 
                 GTCAAAAAGGGATATCAAATGA 
               
               
                   
                   
                   
                  1899 
                 AGTGATGGGGTCACAATGGGGA 
               
               
                   
                   
                   
                   
                 AATTGAAGTGGTGCATAACATTG 
               
               
                   
                   
                   
                   
                 CCAAAATAGTGTGCCACTAGAA 
               
               
                   
                   
                   
                   
                 ATGGTGTAAAG 
               
               
                   
               
               
                 314 
                 MARCKS 
                 NM_023009.5 
                  1117- 
                 TCCAAGTAGGTTTTGTTTACCCT 
               
               
                   
                 L1 
                   
                  1216 
                 ACTCCCCAAATCCCTGAGCCAGA 
               
               
                   
                   
                   
                   
                 AGTGGGGTGCTTATACTCCCAAA 
               
               
                   
                   
                   
                   
                 CCTTGAGTGTCCAGCCTTCCCCT 
               
               
                   
                   
                   
                   
                 GTTGTTTT 
               
               
                   
               
               
                 315 
                 MBD1 
                 NM_015844.2 
                  2380- 
                 TGGCTGCAGGCCTGACTACTGCC 
               
               
                   
                   
                   
                  2479 
                 CACACCAACGAGGTGATCTAGC 
               
               
                   
                   
                   
                   
                 AGATACATGGCAACGTGTGAACT 
               
               
                   
                   
                   
                   
                 GCAACAACGCCTGGTGCCCCAGC 
               
               
                   
                   
                   
                   
                 ACCAACCTT 
               
               
                   
               
               
                 316 
                 C19orf 
                 NM_174918.2 
                  1062- 
                 CATACTAGAGTATACTGCGGCGT 
               
               
                   
                 59 
                   
                  1161 
                 GTTTTCTGTCTACCCATGTCATG 
               
               
                   
                   
                   
                   
                 GTGGGGGAGATTTATCTCCGTAC 
               
               
                   
                   
                   
                   
                 ATGTGGGTGTCGCCATGTGTGCC 
               
               
                   
                   
                   
                   
                 CTGTCACT 
               
               
                   
               
               
                 317 
                 MED16 
                 NM_005481.2 
                  2152- 
                 TCTGAAGCCCAGCTGCCTGCCCG 
               
               
                   
                   
                   
                  2251 
                 TGTATACGGCCACCTCGGATACC 
               
               
                   
                   
                   
                   
                 CAGGACAGCATGTCCCTGCTCTT 
               
               
                   
                   
                   
                   
                 CCGCCTGCTCACCAAGCTCTGGA 
               
               
                   
                   
                   
                   
                 TCTGCTGT 
               
               
                   
               
               
                 318 
                 MEN1 
                 NM_130799.2 
                  2222- 
                 CCCAGCCCCTAGAAACCCAAGCT 
               
               
                   
                   
                   
                  2321 
                 CCTCCTCGGAACCGCTCACCTAG 
               
               
                   
                   
                   
                   
                 AGCCAGACCAACGTTACTCAGG 
               
               
                   
                   
                   
                   
                 GCTCCTCCCAGCTTGTAGGAGCT 
               
               
                   
                   
                   
                   
                 GAGGTTTCA 
               
               
                   
               
               
                 319 
                 MERTK 
                 NM_006343.2 
                   666- 
                 GAAGAGATCGTGTCTGATCCCAT 
               
               
                   
                   
                   
                   765 
                 CTACATCGAAGTACAAGGACTTC 
               
               
                   
                   
                   
                   
                 CTCACTTTACTAAGCAGCCTGAG 
               
               
                   
                   
                   
                   
                 AGCATGAATGTCACCAGAAACA 
               
               
                   
                   
                   
                   
                 CAGCCTTCA 
               
               
                   
               
               
                 320 
                 MFSD1 
                 NM_022736.2 
                  2023- 
                 AAGGGCTGCGTTACACAAAATA 
               
               
                   
                   
                   
                  2122 
                 AACAATGGCATTGTCATAGGCCT 
               
               
                   
                   
                   
                   
                 TCCTTTTACTAGTAGGGCATAAT 
               
               
                   
                   
                   
                   
                 GCTAGGGAATATGTGAAGATGTT 
               
               
                   
                   
                   
                   
                 TTTATGAAG 
               
               
                   
               
               
                 321 
                 MID1IP 
                 NM_021242.5 
                  3472- 
                 AGCTGGCATTTCGCCAGCTTGTA 
               
               
                   
                 1 
                   
                  3571 
                 CGTAGCTTGCCACTCAGTGAAAA 
               
               
                   
                   
                   
                   
                 TAATAACATTATTATGAGAAAGT 
               
               
                   
                   
                   
                   
                 GGACTTAACCGAAATGGAACCA 
               
               
                   
                   
                   
                   
                 ACTGACATT 
               
               
                   
               
               
                 322 
                 MPDU1 
                 NM_004870.3 
                  1226- 
                 CATTCAGCCAAGCCTCCTCCTCT 
               
               
                   
                   
                   
                  1325 
                 AGCAGCAATTTCCAGCTGTGTAA 
               
               
                   
                   
                   
                   
                 CACTATCCTGGGCAAATGTTTTA 
               
               
                   
                   
                   
                   
                 CCCTGTCCTCCAGCCTCCCTGCT 
               
               
                   
                   
                   
                   
                 TCCCTTCT 
               
               
                   
               
               
                 323 
                 MRPL27 
                 NM_148571.1 
                  2189- 
                 TCAAACTGGTAGCTATGCTTTGA 
               
               
                   
                   
                   
                  2288 
                 TGTCCTGTTGAGGCCATCGGACA 
               
               
                   
                   
                   
                   
                 GAGACTGGAGCCCAGGTGACAG 
               
               
                   
                   
                   
                   
                 GAGATGGTGATACCAGAAGTCA 
               
               
                   
                   
                   
                   
                 AGGGTTGGGG 
               
               
                   
               
               
                 324 
                 MRPS16 
                 NM_016065.3 
                  1811- 
                 ATTCAAATGTGGCTGTGATTTCT 
               
               
                   
                   
                   
                  1910 
                 GCATATATCATAGATGGGATCCT 
               
               
                   
                   
                   
                   
                 TCTGAGAATACTGGAATAGGGA 
               
               
                   
                   
                   
                   
                 ATTAGGACACCAAGCCAATTCAG 
               
               
                   
                   
                   
                   
                 CTGTGAACC 
               
               
                   
               
               
                 325 
                 MS4A2 
                 NM_000139.3 
                   662- 
                 TTCTCACCATTCTGGGACTTGGT 
               
               
                   
                   
                   
                   761 
                 AGTGCTGTGTCACTCACAATCTG 
               
               
                   
                   
                   
                   
                 TGGAGCTGGGGAAGAACTCAAA 
               
               
                   
                   
                   
                   
                 GGAAACAAGGTTCCAGAGGATC 
               
               
                   
                   
                   
                   
                 GTGTTTATGA 
               
               
                   
               
               
                 326 
                 MS4A6A 
                 NM_022349.3 
                  1290- 
                 CTGGGAAGTTAAATGACTGGCCT 
               
               
                   
                   
                   
                  1389 
                 GGCATTATGCTATGAGTTTGTGC 
               
               
                   
                   
                   
                   
                 CTTTGCTGAGGACACTAGAACCT 
               
               
                   
                   
                   
                   
                 GGCTTGCCTCCCTTATAAGCAGA 
               
               
                   
                   
                   
                   
                 AACAATTT 
               
               
                   
               
               
                 327 
                 MS4A6A  
                 NM_152851.2 
                   880- 
                 CTGCGGTGGAAACAGGCTTACTC 
               
               
                   
                 b 
                   
                   979 
                 TGACTTCCCTGGGAGTGTACTTT 
               
               
                   
                   
                   
                   
                 TCCTGCCTCACAGTTACATTGGT 
               
               
                   
                   
                   
                   
                 AATTCTGGCATGTCCTCAAAAAT 
               
               
                   
                   
                   
                   
                 GACTCATG 
               
               
                   
               
               
                 328 
                 MTCH1 
                 NM_014341.2 
                  2081- 
                 TCCTCCTCATCTAATGCTCATCT 
               
               
                   
                   
                   
                  2180 
                 GTTTAATGGTGATGCCTCGCGTA 
               
               
                   
                   
                   
                   
                 CAGGATCTGGTTACCTGTGCAGT 
               
               
                   
                   
                   
                   
                 TGTGAATACCCAGAGGTTGGGCA 
               
               
                   
                   
                   
                   
                 GATCAGTG 
               
               
                   
               
               
                 329 
                 MYADM 
                 NM_00102082 
                  2656- 
                 TCTTTTTCCTGGCCATGAGGACA 
               
               
                   
                   
                 0.1 
                  2755 
                 AAAATTACTGAGTGGCCCTTAAA 
               
               
                   
                   
                   
                   
                 GAGGGAAGTTTGTTTTCAGCTGT 
               
               
                   
                   
                   
                   
                 TCTCTTTTGCCCGTAGGTGGGAG 
               
               
                   
                   
                   
                   
                 GGTGGGGA 
               
               
                   
               
               
                 330 
                 MYADM  
                 NM_00102082 
                  2789- 
                 TGAATGTGTAGTGCACACGCACG 
               
               
                   
                 b 
                 0.1 
                  2888 
                 GGTGTTTCTGTGTGCTAGTTGCT 
               
               
                   
                   
                   
                   
                 TCTTGCTGCTGCTTCCTGCTTGT 
               
               
                   
                   
                   
                   
                 CTGGGACTCACATACATAACGTG 
               
               
                   
                   
                   
                   
                 ATATATAT 
               
               
                   
               
               
                 331 
                 C19orf 
                 NM_019107.3 
                   649- 
                 TGTCCCTGAAAGGGCCAGCACAT 
               
               
                   
                 10 
                   
                   748 
                 CACTGGTTTTCTAGGAGGGACTC 
               
               
                   
                   
                   
                   
                 TTAAGTTTTCTACCTGGGCTGAC 
               
               
                   
                   
                   
                   
                 GTTGCCTTGTCCGGAGGGGCTTG 
               
               
                   
                   
                   
                   
                 CAGGGTGG 
               
               
                   
               
               
                 332 
                 MYL12A 
                 NM_006471.3 
                   305- 
                 TCTCTGGGTAGCAGGGTGGTGTG 
               
               
                   
                   
                   
                   404 
                 ATAGCGGCAGCGAGGGGCTCGG 
               
               
                   
                   
                   
                   
                 AGAGGTGCTCGGATTCTCGTAGC 
               
               
                   
                   
                   
                   
                 TGTGCCGGGACTTAACCACCACC 
               
               
                   
                   
                   
                   
                 ATGTCGAGC 
               
               
                   
               
               
                 333 
                 MYLIP 
                 NM_013262.3 
                  2701- 
                 TTGGGCATTTTGGAAGCTGGTCA 
               
               
                   
                   
                   
                  2800 
                 GCTAGCAGGTTTTCTGGGATGTC 
               
               
                   
                   
                   
                   
                 GGGAGACCTAGATGACCTTATCG 
               
               
                   
                   
                   
                   
                 GGTGCAATACTAGCTAAGGTAA 
               
               
                   
                   
                   
                   
                 AGCTAGAAA 
               
               
                   
               
               
                 334 
                 NAT5 
                 NM_181528.3 
                   735- 
                 AAACATACCACTCTCATGGTTCA 
               
               
                   
                   
                   
                   834 
                 TAGTATTCACTGTATGTATGCTA 
               
               
                   
                   
                   
                   
                 GGGAAAAGACTTGCTCCAGTCTC 
               
               
                   
                   
                   
                   
                 CTCCTCAGTTCTGTGCCTGAGAA 
               
               
                   
                   
                   
                   
                 CCACTGCT 
               
               
                   
               
               
                 335 
                 NADK 
                 NM_023018.4 
                  2449- 
                 TCCGGGGCTAGTGATCGTGATCC 
               
               
                   
                   
                   
                  2548 
                 CTTTTATTTGCAACTGTAATGAG 
               
               
                   
                   
                   
                   
                 AATTTTTCACACTAACACAGCGA 
               
               
                   
                   
                   
                   
                 GGGACTCAACACGCTGATTCTCC 
               
               
                   
                   
                   
                   
                 TCCTGCCT 
               
               
                   
               
               
                 336 
                 NAGK 
                 NM_017567.4 
                  1362- 
                 GGGCCAGGCACATCGGGCACCT 
               
               
                   
                   
                   
                  1461 
                 CCTCCCCATGGACTATAGCGCCA 
               
               
                   
                   
                   
                   
                 ATGCCATTGCCTTCTATTCCTAC 
               
               
                   
                   
                   
                   
                 ACCTTTTCCTAGGGGGCTGGTCC 
               
               
                   
                   
                   
                   
                 CGGCTCCAC 
               
               
                   
               
               
                 337 
                 NCAPG 
                 NM_022346.4 
                  3080- 
                 ACCCAAGCATCAAAGTCTACTCA 
               
               
                   
                   
                   
                  3179 
                 GCTAAAGACTAACAGAGGACAG 
               
               
                   
                   
                   
                   
                 AGAAAAGTGACAGTTTCAGCTA 
               
               
                   
                   
                   
                   
                 GGACGAACAGGAGGTGTCAGAC 
               
               
                   
                   
                   
                   
                 TGCTGAAGCCG 
               
               
                   
               
               
                 338 
                 NCOA5 
                 NM_020967.2 
                  2837- 
                 TGGACATGTTCTCGAGATGGGTG 
               
               
                   
                   
                   
                  2936 
                 GCTGTTCGCGACTTTTGTACCAG 
               
               
                   
                   
                   
                   
                 AGTGAAATTGTTAGAAGGAGGG 
               
               
                   
                   
                   
                   
                 TTTCTGGCTGTGGTTCTAAATGG 
               
               
                   
                   
                   
                   
                 AGCCCCAGG 
               
               
                   
               
               
                 339 
                 NCR1 
                 NM_004829.5 
                   603- 
                 CGATGTTTTGGCTCCTATAACAA 
               
               
                   
                   
                   
                   702 
                 CCATGCCTGGTCTTTCCCCAGTG 
               
               
                   
                   
                   
                   
                 AGCCAGTGAAGCTCCTGGTCACA 
               
               
                   
                   
                   
                   
                 GGCGACATTGAGAACACCAGCC 
               
               
                   
                   
                   
                   
                 TTGCACCTG 
               
               
                   
               
               
                 340 
                 NDRG2 
                 NM_016250.2 
                  1516- 
                 TATGCATCCTCTGTCCTGATCTA 
               
               
                   
                   
                   
                  1615 
                 GGTGTCTATAGCTGAGGGGTAAG 
               
               
                   
                   
                   
                   
                 AGGTTGTTGTAGTTGTCCTGGTG 
               
               
                   
                   
                   
                   
                 CCTCCATCAGACTCTCCCTACTT 
               
               
                   
                   
                   
                   
                 GTCCCATA 
               
               
                   
               
               
                 341 
                 NDUFA4 
                 NM_002489.3 
                   262- 
                 TGGGACAGAAATAACCCAGAGC 
               
               
                   
                   
                   
                   361 
                 CCTGGAACAAACTGGGTCCCAAT 
               
               
                   
                   
                   
                   
                 GATCAATACAAGTTCTACTCAGT 
               
               
                   
                   
                   
                   
                 GAATGTGGATTACAGCAAGCTG 
               
               
                   
                   
                   
                   
                 AAGAAGGAAC 
               
               
                   
               
               
                 342 
                 NDUFAF 
                 NM_174889.4 
                   486- 
                 TCCTGCCTCCACCAGTTCAAACT 
               
               
                   
                 2 
                   
                   585 
                 CAAATTAAAGGCCATGCCTCTGC 
               
               
                   
                   
                   
                   
                 TCCATACTTTGGAAAGGAAGAAC 
               
               
                   
                   
                   
                   
                 CCTCAGTGGCTCCCAGCAGCACT 
               
               
                   
                   
                   
                   
                 GGTAAAAC 
               
               
                   
               
               
                 343 
                 NDUFB3 
                 NM_002491.2 
                   383- 
                 ACAATGGAAGATAGAAGGGACA 
               
               
                   
                   
                   
                   482 
                 CCATTAGAAACTATCCAGAAGA 
               
               
                   
                   
                   
                   
                 AGCTGGCTGCAAAAGGGCTAAG 
               
               
                   
                   
                   
                   
                 GGATCCATGGGGCCGCAATGAA 
               
               
                   
                   
                   
                   
                 GCTTGGAGATAC 
               
               
                   
               
               
                 344 
                 NDUFS4 
                 NM_002495.2 
                   326- 
                 GAGTTTGATACCAGAGAGCGAT 
               
               
                   
                   
                   
                   425 
                 GGGAAAATCCTTTGATGGGTTGG 
               
               
                   
                   
                   
                   
                 GCATCAACGGCTGATCCCTTATC 
               
               
                   
                   
                   
                   
                 CAACATGGTTCTAACCTTCAGTA 
               
               
                   
                   
                   
                   
                 CTAAAGAAG 
               
               
                   
               
               
                 345 
                 NDUFV2 
                 NM_021074.4 
                   687- 
                 TTACTATGAGGATTTGACAGCTA 
               
               
                   
                   
                   
                   786 
                 AGGATATTGAAGAAATTATTGAT 
               
               
                   
                   
                   
                   
                 GAGCTCAAGGCTGGCAAAATCC 
               
               
                   
                   
                   
                   
                 CAAAACCAGGGCCAAGGAGTGG 
               
               
                   
                   
                   
                   
                 ACGCTTCTCT 
               
               
                   
               
               
                 346 
                 NFAT5 
                 NM_138713.3 
                  3857- 
                 CCCAAGAAGCATTTTTTGCAGCA 
               
               
                   
                   
                   
                  3956 
                 CCGAACTCAATTTCTCCACTTCA 
               
               
                   
                   
                   
                   
                 GTCAACATCAAACAGTGAACAA 
               
               
                   
                   
                   
                   
                 CAAGCTGCTTTCCAACAGCAAGC 
               
               
                   
                   
                   
                   
                 TCCAATATC 
               
               
                   
               
               
                 347 
                 NFATC1 
                 NM_172389.1 
                  1985- 
                 CGAATTCTCTGGTGGTTGAGATC 
               
               
                   
                   
                   
                  2084 
                 CCGCCATTTCGGAATCAGAGGAT 
               
               
                   
                   
                   
                   
                 AACCAGCCCCGTTCACGTCAGTT 
               
               
                   
                   
                   
                   
                 TCTACGTCTGCAACGGGAAGAG 
               
               
                   
                   
                   
                   
                 AAAGCGAAG 
               
               
                   
               
               
                 348 
                 NFATC4 
                 NM_00113602 
                  2297- 
                 ACAAGAGGGTTTCCCGGCCAGTC 
               
               
                   
                   
                 2.2 
                  2396 
                 CAGGTCTACTTTTATGTCTCCAA 
               
               
                   
                   
                   
                   
                 TGGGCGGAGGAAACGCAGTCCT 
               
               
                   
                   
                   
                   
                 ACCCAGAGTTTCAGGTTTCTGCC 
               
               
                   
                   
                   
                   
                 TGTGATCTG 
               
               
                   
               
               
                 349 
                 NFKB1 
                 NM_003998.3 
                  3606- 
                 CGGATGCATCTGGGGATGAGGTT 
               
               
                   
                   
                   
                  3705 
                 GCTTACTAAGCTTTGCCAGCTGC 
               
               
                   
                   
                   
                   
                 TGCTGGATCACAGCTGCTTTCTG 
               
               
                   
                   
                   
                   
                 TTGTCATTGCTGTTGTCCCTCTG 
               
               
                   
                   
                   
                   
                 CTACGTTC 
               
               
                   
               
               
                 350 
                 NFKB2 
                 NM_002502.2 
                   826- 
                 ATCTCCGGGGGCATCAAACCTGA 
               
               
                   
                   
                   
                   925 
                 AGATTTCTCGAATGGACAAGACA 
               
               
                   
                   
                   
                   
                 GCAGGCTCTGTGCGGGGTGGAG 
               
               
                   
                   
                   
                   
                 ATGAAGTTTATCTGCTTTGTGAC 
               
               
                   
                   
                   
                   
                 AAGGTGCAG 
               
               
                   
               
               
                 351 
                 NIPBL 
                 NM_133433.3 
                  8755- 
                 GCGCCGTGATGGCCGCAAACTG 
               
               
                   
                   
                   
                  8854 
                 GTGCCTTGGGTAGACACTATTAA 
               
               
                   
                   
                   
                   
                 AGAGTCAGACATTATTTACAAAA 
               
               
                   
                   
                   
                   
                 AAATTGCTCTAACGAGTGCTAAT 
               
               
                   
                   
                   
                   
                 AAGCTGACT 
               
               
                   
               
               
                 352 
                 NLRP3 
                 NM_00107982 
                   416- 
                 AGTGGGGTTCAGATAATGCACGT 
               
               
                   
                   
                 1.2 
                   515 
                 GTTTCGAATCCCACTGTGATATG 
               
               
                   
                   
                   
                   
                 CCAGGAAGACAGCATTGAAGAG 
               
               
                   
                   
                   
                   
                 GAGTGGATGGGTTTACTGGAGTA 
               
               
                   
                   
                   
                   
                 CCTTTCGAG 
               
               
                   
               
               
                 353 
                 NME1- 
                 NM_00101813 
                   484- 
                 ACCTGGAGCGCACCTTCATCGCC 
               
               
                   
                 NME2 
                 6.2 
                   583 
                 ATCAAGCCGGACGGCGTGCAGC 
               
               
                   
                   
                   
                   
                 GCGGCCTGGTGGGCGAGATCATC 
               
               
                   
                   
                   
                   
                 AAGCGCTTCGAGCAGAAGGGAT 
               
               
                   
                   
                   
                   
                 TCCGCCTCGT 
               
               
                   
               
               
                 354 
                 NUDT18 
                 NM_024815.3 
                  1369- 
                 CCCCAGTGGCATCTCCTCATCAC 
               
               
                   
                   
                   
                  1468 
                 GTTCTGTGCCGTCCTTGGGAAAG 
               
               
                   
                   
                   
                   
                 GCCTGCATTCTGATCCTTCCAGG 
               
               
                   
                   
                   
                   
                 CCCTTCGAGCATGGAGGGGCACT 
               
               
                   
                   
                   
                   
                 GGGGAAGG 
               
               
                   
               
               
                 355 
                 NUMB 
                 NM_00100574 
                  2833- 
                 CATAAGATTGATTTATCATTGAT 
               
               
                   
                   
                 4.1 
                  2932 
                 GCCTACTGAAATAAAAAGAGGA 
               
               
                   
                   
                   
                   
                 AAGGCTGGAAGCTGCAGACAGG 
               
               
                   
                   
                   
                   
                 ATCCCTAGCTTGTTTTCTGTCAG 
               
               
                   
                   
                   
                   
                 TCATTCATTG 
               
               
                   
               
               
                 356 
                 NUP153 
                 NM_005124.3 
                  5104- 
                 TTTATGATCCAGCAGATTATTCA 
               
               
                   
                   
                   
                  5203 
                 CTGATTTGACATAGTCTGGCTGT 
               
               
                   
                   
                   
                   
                 ACCCAGGAATGGAGCCTGCACG 
               
               
                   
                   
                   
                   
                 GTGAATGGCTTTGTATAGAACCT 
               
               
                   
                   
                   
                   
                 CTTTGTCTA 
               
               
                   
               
               
                 357 
                 OLR1 
                 NM_002543.3 
                  1524- 
                 ACACATTTTGGGACAAGTGGGG 
               
               
                   
                   
                   
                  1623 
                 AGCCCAAGAAAGTAATTAGTAA 
               
               
                   
                   
                   
                   
                 GTGAGTGGTCTTTTCTGTAAGCT 
               
               
                   
                   
                   
                   
                 AATCCACAACCTGTTACCACTTC 
               
               
                   
                   
                   
                   
                 CTGAATCAGT 
               
               
                   
               
               
                 358 
                 OSBP 
                 ILMN_170637 
                   130- 
                 TTCTCTTCCTTCACCATCTGCAC 
               
               
                   
                   
                 6.1 
                   229 
                 TACATTTCTGGCTGATCCCAATC 
               
               
                   
                   
                   
                   
                 AGATTCCCGCTAATGGAAGAAGT 
               
               
                   
                   
                   
                   
                 TTAGAATCTTTCAGGTGGAATAA 
               
               
                   
                   
                   
                   
                 AGTCACAT 
               
               
                   
               
               
                 359 
                 FAM105 
                 NM_138348.4 
                  2537- 
                 TGCAGATGGTGTTCACATGAACC 
               
               
                   
                 B 
                   
                  2636 
                 GGAGACATCACTCTTTAGGATTC 
               
               
                   
                   
                   
                   
                 TACTGGCAGCCCCTGAATTGGCT 
               
               
                   
                   
                   
                   
                 CAACGTTTGTGGAGGTGGTATTT 
               
               
                   
                   
                   
                   
                 CCCTGAAG 
               
               
                   
               
               
                 360 
                 P2RY10 
                 NM_198333.1 
                   972- 
                 TTACACCATGGTAAAGGAAACC 
               
               
                   
                   
                   
                  1071 
                 ATCATTAGCAGTTGTCCCGTTGT 
               
               
                   
                   
                   
                   
                 CCGAATCGCACTGTATTTCCACC 
               
               
                   
                   
                   
                   
                 CTTTTTGCCTGTGCCTTGCAAGT 
               
               
                   
                   
                   
                   
                 CTCTGCTGC 
               
               
                   
               
               
                 361 
                 PACS1 
                 NM_018026.3 
                  3830- 
                 CGCTGTCTTCGTGGCTTCCACCC 
               
               
                   
                   
                   
                  3929 
                 TTGTTAATGATGCTCCTGCCTCT 
               
               
                   
                   
                   
                   
                 GCCTCCCAGCCCCTCACCCAGCA 
               
               
                   
                   
                   
                   
                 CAGCTCTGCCTGGACTTGGAGAG 
               
               
                   
                   
                   
                   
                 ATGGGAGG 
               
               
                   
               
               
                 362 
                 PANK2 
                 NM_153640.2 
                   824- 
                 AGTGGATAAACTAGTACGAGAT 
               
               
                   
                   
                   
                   923 
                 ATTTATGGAGGGGACTATGAGA 
               
               
                   
                   
                   
                   
                 GGTTTGGACTGCCAGGCTGGGCT 
               
               
                   
                   
                   
                   
                 GTGGCTTCAAGCTTTGGAAACAT 
               
               
                   
                   
                   
                   
                 GATGAGCAAG 
               
               
                   
               
               
                 363 
                 PDCD10 
                 NM_145859.1 
                   901- 
                 AAGAGATGTACTTCTCAGTGGCA 
               
               
                   
                   
                   
                  1000 
                 GTATTGAACTGCCTTTATCTGTA 
               
               
                   
                   
                   
                   
                 AATTTTAAAGTTTGACTGTATAA 
               
               
                   
                   
                   
                   
                 ATTATCAGTCCCTCCTGAAGGGA 
               
               
                   
                   
                   
                   
                 TCTAATCC 
               
               
                   
               
               
                 364 
                 PDGFD 
                 NM_033135.3 
                  3394- 
                 CCTGTGAAAACATCAGTTTCCTG 
               
               
                   
                   
                   
                  3493 
                 TACCAAAGTCAAAATGAACGTTA 
               
               
                   
                   
                   
                   
                 CATCACTCTAACCTGAACAGCTC 
               
               
                   
                   
                   
                   
                 ACAATGTAGCTGTAAATATAAAA 
               
               
                   
                   
                   
                   
                 AATGAGAG 
               
               
                   
               
               
                 365 
                 PDSS1 
                 NM_014317.3 
                  1199- 
                 CATGAAGCAATAAGAGAGATCA 
               
               
                   
                   
                   
                  1298 
                 GTAAACTTCGACCATCCCCAGAA 
               
               
                   
                   
                   
                   
                 AGAGATGCCCTCATTCAGCTTTC 
               
               
                   
                   
                   
                   
                 AGAAATTGTACTCACAAGAGAT 
               
               
                   
                   
                   
                   
                 AAATGACAAC 
               
               
                   
               
               
                 366 
                 PELP1 
                 NM_014389.2 
                  1989- 
                 TGGCCCCGTCTCCTCGCTGCCCA 
               
               
                   
                   
                   
                  2088 
                 CCTCCTCTTGCCTGTGCCCTGCA 
               
               
                   
                   
                   
                   
                 AGCCTTCTCCCTCGGCCAGCGAG 
               
               
                   
                   
                   
                   
                 AAGATAGCCTTGAGGTCTCCTCT 
               
               
                   
                   
                   
                   
                 TTCTGCTC 
               
               
                   
               
               
                 367 
                 PFAS 
                 NM_012393.2 
                  5109- 
                 CATCCCTAGATCCTAACCCTTTA 
               
               
                   
                   
                   
                  5208 
                 GTATGCTGGAATTCTACTCTTCA 
               
               
                   
                   
                   
                   
                 CTTACTGCATTGACTGTTGTTGA 
               
               
                   
                   
                   
                   
                 TTAGTTATTATTGCAAAGCACTG 
               
               
                   
                   
                   
                   
                 TCACCGGC 
               
               
                   
               
               
                 368 
                 PFDN5 
                 NM_145897.2 
                   232- 
                 ATCGATGTGGGAACTGGGTACTA 
               
               
                   
                   
                   
                   331 
                 TGTAGAGAAGACAGCTGAGGAT 
               
               
                   
                   
                   
                   
                 GCCAAGGACTTCTTCAAGAGGA 
               
               
                   
                   
                   
                   
                 AGATAGATTTTCTAACCAAGCAG 
               
               
                   
                   
                   
                   
                 ATGGAGAAAA 
               
               
                   
               
               
                 369 
                 PFDN5  
                 NM_145897.2 
                   331- 
                 ATCCAACCAGCTCTTCAGGAGAA 
               
               
                   
                 b 
                   
                   430 
                 GCACGCCATGAAACAGGCCGTC 
               
               
                   
                   
                   
                   
                 ATGGAAATGATGAGTCAGAAGA 
               
               
                   
                   
                   
                   
                 TTCAGCAGCTCACAGCCCTGGGG 
               
               
                   
                   
                   
                   
                 GCAGCTCAGG 
               
               
                   
               
               
                 370 
                 PGK1 
                 NM_000291.3 
                  1122- 
                 GTCCTGAAAGCAGCAAGAAGTA 
               
               
                   
                   
                   
                  1221 
                 TGCTGAGGCTGTCACTCGGGCTA 
               
               
                   
                   
                   
                   
                 AGCAGATTGTGTGGAATGGTCCT 
               
               
                   
                   
                   
                   
                 GTGGGGGTATTTGAATGGGAAG 
               
               
                   
                   
                   
                   
                 CTTTTGCCCG 
               
               
                   
               
               
                 371 
                 PHF8 
                 NM_015107.2 
                  5704- 
                 ATCAAGGTTTAGAACACCATGAG 
               
               
                   
                   
                   
                  5803 
                 ATAGTTACCCCTGATCTCCAGTC 
               
               
                   
                   
                   
                   
                 CCTAGCTGGGGGCTGGACAGGG 
               
               
                   
                   
                   
                   
                 GGAAGGGAGAGAGGATTTCTAT 
               
               
                   
                   
                   
                   
                 TCACCTTTAA 
               
               
                   
               
               
                 372 
                 PHLPP2 
                 NM_015020.3 
                  7601- 
                 CCAGTTGGGTGTGGCAGATCTAC 
               
               
                   
                   
                   
                  7700 
                 TGAATATCAAATGATGCTCTTCT 
               
               
                   
                   
                   
                   
                 TCCCATGTAGACCTTCAGCAAAA 
               
               
                   
                   
                   
                   
                 GCCGGTACTTGGAAGCCACAGG 
               
               
                   
                   
                   
                   
                 CTCACCTTC 
               
               
                   
               
               
                 373 
                 PHRF1 
                 NM_020901.3 
                  5239- 
                 GGGAAATGGGGGGCATCACCAT 
               
               
                   
                   
                   
                  5338 
                 GCCTGCCGTCGGGTTCCTGCGCT 
               
               
                   
                   
                   
                   
                 GACACCTGGTCTGTGCACCTGTG 
               
               
                   
                   
                   
                   
                 TTGCTCACAGTTGAAAACTGGAC 
               
               
                   
                   
                   
                   
                 ACTTTTGTA 
               
               
                   
               
               
                 374 
                 PI4K2A 
                 NM_018425.3 
                  3886- 
                 TCCATGGAATTGCTGAGACGTGG 
               
               
                   
                   
                   
                  3985 
                 CTCCTGGGGCTATTTCTCCCTAA 
               
               
                   
                   
                   
                   
                 TAAAGGATGATCCAGGTCCTCAT 
               
               
                   
                   
                   
                   
                 TTCCAAAGTCCCAATGCTCTGAA 
               
               
                   
                   
                   
                   
                 AACCAAAA 
               
               
                   
               
               
                 375 
                 PIK3CD 
                 NM_005026.3 
                  4799- 
                 GAGCCAGAAGTAGCCGCCCGCT 
               
               
                   
                   
                   
                  4898 
                 CAGCGGCTCAGGTGCCAGCTCTG 
               
               
                   
                   
                   
                   
                 TTCTGATTCACCAGGGGTCCGTC 
               
               
                   
                   
                   
                   
                 AGTAGTCATTGCCACCCGCGGGG 
               
               
                   
                   
                   
                   
                 CACCTCCCT 
               
               
                   
               
               
                 376 
                 PIM2 
                 NM_006875.3 
                  1947- 
                 TTTTTGGGGGATGGGCTAGGGGA 
               
               
                   
                   
                   
                  2046 
                 AATAAGGCTTGCTGTTTGTTCTC 
               
               
                   
                   
                   
                   
                 CTGGGGCGCTCCCTCCAACTTTT 
               
               
                   
                   
                   
                   
                 GCAGATTCTTGCAACCTCCTCCT 
               
               
                   
                   
                   
                   
                 GAGCCGGG 
               
               
                   
               
               
                 377 
                 PLAC8 
                 NM_00113071 
                   289- 
                 CTGATATGAATGAATGCTGTCTG 
               
               
                   
                   
                 5.1 
                   388 
                 TGTGGAACAAGCGTCGCAATGA 
               
               
                   
                   
                   
                   
                 GGACTCTCTACAGGACCCGATAT 
               
               
                   
                   
                   
                   
                 GGCATCCCTGGATCTATTTGTGA 
               
               
                   
                   
                   
                   
                 TGACTATAT 
               
               
                   
               
               
                 378 
                 PLEKHG 
                 NM_015432.3 
                  6365- 
                 CCAGTTGTGGGTTAAGAATAGGC 
               
               
                   
                 4 
                   
                  6464 
                 TAGAGCAGACATTGGGTGTTTCC 
               
               
                   
                   
                   
                   
                 ATGCTGTAGGCTGGTGGGGGACC 
               
               
                   
                   
                   
                   
                 ATGTGCCTCTAGGCAGTGACTAG 
               
               
                   
                   
                   
                   
                 GGTGCCCC 
               
               
                   
               
               
                 379 
                 POLR2A 
                 NM_000937.4 
                  6539- 
                 CCCCTGCCTGTCCCCAAATTGAA 
               
               
                   
                   
                   
                  6638 
                 GATCCTTCCTTGCCTGTGGCTTG 
               
               
                   
                   
                   
                   
                 ATGCGGGGCGGGTAAAGGGTAT 
               
               
                   
                   
                   
                   
                 TTTAACTTAGGGGTAGTTCCTGC 
               
               
                   
                   
                   
                   
                 TGTGAGTGG 
               
               
                   
               
               
                 380 
                 PPP1R3 
                 XM_927029.1 
                  4342- 
                 CAGAACCTCCTCAGTTCCTTCAC 
               
               
                   
                 E 
                   
                  4441 
                 AGTGCAACCCTGTGTACTTGGCC 
               
               
                   
                   
                   
                   
                 CGCAACCCAATAGTATTGTGCCT 
               
               
                   
                   
                   
                   
                 CACTTCACCTTCCATGGGCAACT 
               
               
                   
                   
                   
                   
                 GCCCTCCC 
               
               
                   
               
               
                 381 
                 PPP2R5 
                 NM_178588.1 
                   941- 
                 ACAGCACCCTCACGGAACCAGT 
               
               
                   
                 C 
                   
                  1040 
                 GGTGATGGCACTTCTCAAATACT 
               
               
                   
                   
                   
                   
                 GGCCAAAGACTCACAGTCCAAA 
               
               
                   
                   
                   
                   
                 AGAAGTAATGTTCTTAAACGAAT 
               
               
                   
                   
                   
                   
                 TAGAAGAGAT 
               
               
                   
               
               
                 382 
                 PPP6C 
                 NM_002721.4 
                  1536- 
                 TTAAGAAATTTCAGCAGCAAAGT 
               
               
                   
                   
                   
                  1635 
                 TGTTATTCAGTGGGCACGATGGA 
               
               
                   
                   
                   
                   
                 CTCCAAATGCCTCAAGTTATGTA 
               
               
                   
                   
                   
                   
                 TACCTGTCCCAGATGTAAACTTC 
               
               
                   
                   
                   
                   
                 ATTGTCCT 
               
               
                   
               
               
                 383 
                 PRG2 
                 NM_002728.4 
                   257- 
                 CTCTGGAAGTGAAGATGCCTCCA 
               
               
                   
                   
                   
                   356 
                 AGAAAGATGGGGCTGTTGAGTCT 
               
               
                   
                   
                   
                   
                 ATCTCAGTGCCAGATATGGTGGA 
               
               
                   
                   
                   
                   
                 CAAAAACCTTACGTGTCCTGAGG 
               
               
                   
                   
                   
                   
                 AAGAGGAC 
               
               
                   
               
               
                 384 
                 PRPF3 
                 NM_004698.2 
                  2116- 
                 CCTACAGAGAACATGGCTCGTGA 
               
               
                   
                   
                   
                  2215 
                 GCATTTCAAAAAGCATGGGGCTG 
               
               
                   
                   
                   
                   
                 AACACTACTGGGACCTTGCGCTG 
               
               
                   
                   
                   
                   
                 AGTGAATCTGTGTTAGAGTCCAC 
               
               
                   
                   
                   
                   
                 TGATTGAG 
               
               
                   
               
               
                 385 
                 PRPF8 
                 NM_006445.3 
                  7091- 
                 ACTCTGCGGATCGGGAGGACCTG 
               
               
                   
                   
                   
                  7190 
                 TATGCCTGACCGTTTCCCTGCCT 
               
               
                   
                   
                   
                   
                 CCTGCTTCAGCCTCCCGAGGCCG 
               
               
                   
                   
                   
                   
                 AAGCCTCAGCCCCTCCAGACAGG 
               
               
                   
                   
                   
                   
                 CCGCTGAC 
               
               
                   
               
               
                 386 
                 C22orf 
                 NM_173566.2 
                 10495- 
                 CCCGTTGAGCTGGCCATCTAGTG 
               
               
                   
                 30 
                   
                 10594 
                 CAGTGTGCTCTCAGATTCCATGT 
               
               
                   
                   
                   
                   
                 TTGTTGATTGTGTGTCTTCACAA 
               
               
                   
                   
                   
                   
                 GCCCCTCTCTGGTGCTGAATTGG 
               
               
                   
                   
                   
                   
                 ATTTGAAT 
               
               
                   
               
               
                 387 
                 BAT2D1 
                 NM_015172.3 
                  9620- 
                 AGAACAGTGAGTACCTAGAACT 
               
               
                   
                   
                   
                  9719 
                 GTGCCACTAATTAAAGGAAATCC 
               
               
                   
                   
                   
                   
                 TAAGAAGGTGCATTTCTTTACAG 
               
               
                   
                   
                   
                   
                 AGCTGTGTCATGCCATCCTTTGG 
               
               
                   
                   
                   
                   
                 GCCCTCTGC 
               
               
                   
               
               
                 388 
                 PRRG4 
                 NM_024081.5 
                   761- 
                 GAAGACCTGAGGAGGCTGCCTT 
               
               
                   
                   
                   
                   860 
                 GTCTCCATTGCCGCCTTCTGTGG 
               
               
                   
                   
                   
                   
                 AGGATGCAGGATTACCTTCTTAT 
               
               
                   
                   
                   
                   
                 GAACAGGCAGTGGCGCTGACCA 
               
               
                   
                   
                   
                   
                 GAAAACACAG 
               
               
                   
               
               
                 389 
                 PSMA3 
                 NM_152132.2 
                   422- 
                 CTTTGGCTACAACATTCCACTAA 
               
               
                   
                   
                   
                   521 
                 AACATCTTGCAGACAGAGTGGCC 
               
               
                   
                   
                   
                   
                 ATGTATGTGCATGCATATACACT 
               
               
                   
                   
                   
                   
                 CTACAGTGCTGTTAGACCTTTTG 
               
               
                   
                   
                   
                   
                 GCTGCAGT 
               
               
                   
               
               
                 390 
                 PSMA4 
                 NM_002789.3 
                   541- 
                 GTACATTGGCTGGGATAAGCACT 
               
               
                   
                   
                   
                   640 
                 ATGGCTTTCAGCTCTATCAGAGT 
               
               
                   
                   
                   
                   
                 GACCCTAGTGGAAATTACGGGG 
               
               
                   
                   
                   
                   
                 GATGGAAGGCCACATGCATTGG 
               
               
                   
                   
                   
                   
                 AAATAATAGC 
               
               
                   
               
               
                 391 
                 PSMA4  
                 NM_002789.4 
                   879- 
                 GAGGAAGAAGAAGCCAAAGCTG 
               
               
                   
                 b 
                   
                   978 
                 AGCGTGAGAAGAAAGAAAAAGA 
               
               
                   
                   
                   
                   
                 ACAGAAAGAAAAGGATAAATAG 
               
               
                   
                   
                   
                   
                 AATCAGAGATTTTATTACTCATT 
               
               
                   
                   
                   
                   
                 TGGGGCACCAT 
               
               
                   
               
               
                 392 
                 PSMA6 
                 NM_002791.2 
                   218- 
                 GGTCGGCTCTACCAAGTAGAATA 
               
               
                   
                   
                   
                   317 
                 TGCTTTTAAGGCTATTAACCAGG 
               
               
                   
                   
                   
                   
                 GTGGCCTTACATCAGTAGCTGTC 
               
               
                   
                   
                   
                   
                 AGAGGGAAAGACTGTGCAGTAA 
               
               
                   
                   
                   
                   
                 TTGTCACAC 
               
               
                   
               
               
                 393 
                 PSMA6  
                 NM_002791.2 
                   866- 
                 GATGCTCACCTTGTTGCTCTAGC 
               
               
                   
                 b 
                   
                   965 
                 AGAGAGAGACTAAACATTGTCG 
               
               
                   
                   
                   
                   
                 TTAGTTTACCAGATCCGTGATGC 
               
               
                   
                   
                   
                   
                 CACTTACCTGTGTGTTTGGTAAC 
               
               
                   
                   
                   
                   
                 AACAAACCA 
               
               
                   
               
               
                 394 
                 PSMB1 
                 NM_002793.3 
                   687- 
                 GCGGCTGGTGAAAGATGTCTTCA 
               
               
                   
                   
                   
                   786 
                 TTTCTGCGGCTGAGAGAGATGTG 
               
               
                   
                   
                   
                   
                 TACACTGGGGACGCACTCCGGAT 
               
               
                   
                   
                   
                   
                 CTGCATAGTGACCAAAGAGGGC 
               
               
                   
                   
                   
                   
                 ATCAGGGAG 
               
               
                   
               
               
                 395 
                 PSMB7 
                 NM_002799.2 
                   421- 
                 GTTACATTGGTGCAGCCCTAGTT 
               
               
                   
                   
                   
                   520 
                 TTAGGGGGAGTAGATGTTACTGG 
               
               
                   
                   
                   
                   
                 ACCTCACCTCTACAGCATCTATC 
               
               
                   
                   
                   
                   
                 CTCATGGATCAACTGATAAGTTG 
               
               
                   
                   
                   
                   
                 CCTTATGT 
               
               
                   
               
               
                 396 
                 PSMB8 
                 NM_004159.4 
                  1216- 
                 ACTCACAGAGACAGCTATTCTGG 
               
               
                   
                   
                   
                  1315 
                 AGGCGTTGTCAATATGTACCACA 
               
               
                   
                   
                   
                   
                 TGAAGGAAGATGGTTGGGTGAA 
               
               
                   
                   
                   
                   
                 AGTAGAAAGTACAGATGTCAGT 
               
               
                   
                   
                   
                   
                 GACCTGCTGC 
               
               
                   
               
               
                 397 
                 PSMC1 
                 NM_002802.2 
                  1487- 
                 CATCCTGTGTCTTTTGGAGTACG 
               
               
                   
                   
                   
                  1586 
                 ATGTGTAAGTGCCCATTGGGTGG 
               
               
                   
                   
                   
                   
                 CCTGTTGGTCACTGTGCAGCAGT 
               
               
                   
                   
                   
                   
                 CTGCTTCCCAATAAAGCGTGCTC 
               
               
                   
                   
                   
                   
                 TTTCACAA 
               
               
                   
               
               
                 398 
                 PSMD7 
                 NM_002811.4 
                  1231- 
                 GAGCTCTCTGCCTCCGGTCACTC 
               
               
                   
                   
                   
                  1330 
                 TTGCTGTGGTGCTACGTGGAAGT 
               
               
                   
                   
                   
                   
                 GAATGGAGACTGATCTCAAATCT 
               
               
                   
                   
                   
                   
                 GAACTGCAGCTTTCGCTGCTGTG 
               
               
                   
                   
                   
                   
                 AGTTGGGG 
               
               
                   
               
               
                 399 
                 PSME3 
                 NM_005789.3 
                  3203- 
                 TCCCGAGTGATACCCATGAACTG 
               
               
                   
                   
                   
                  3302 
                 CCAGTAGAGGCTGCTATCGTTCC 
               
               
                   
                   
                   
                   
                 ATGTGTAAGGAATGAACTGGTTC 
               
               
                   
                   
                   
                   
                 AAGGCGCGTCCTACCCAGTCATT 
               
               
                   
                   
                   
                   
                 TTCTTTAC 
               
               
                   
               
               
                 400 
                 PTGDR 
                 NM_000953.2 
                  2341- 
                 TATGATGACTGAAAGGGAAAAG 
               
               
                   
                   
                   
                  2440 
                 TGGAGGAAACGCAGCTGCAACT 
               
               
                   
                   
                   
                   
                 GAAGCGGAGACTCTAAACCCAG 
               
               
                   
                   
                   
                   
                 CTTGCAGGTAAGAGCTTTCACCT 
               
               
                   
                   
                   
                   
                 TTGGTAAAAGA 
               
               
                   
               
               
                 401 
                 PTGDR2 
                 NM_004778.1 
                  1836- 
                 GCCAATGCTTACTGCGCTAGACG 
               
               
                   
                   
                   
                  1935 
                 CTTCATCCCACAATCTTAAGGGG 
               
               
                   
                   
                   
                   
                 CAGCTTCTATTAGCCAGTCTTTA 
               
               
                   
                   
                   
                   
                 CAGCTGAGCACATTCTGGCTCAG 
               
               
                   
                   
                   
                   
                 GGAGGTTA 
               
               
                   
               
               
                 402 
                 PUM1 
                 NM_00102065 
                  3753- 
                 AAATGTTCTAGTGTAGAGTCTGA 
               
               
                   
                   
                 8.1 
                  3852 
                 GACGGGCAAGTGGTTGCTCCAG 
               
               
                   
                   
                   
                   
                 GATTACTCCCTCCTCCAAAAAAG 
               
               
                   
                   
                   
                   
                 GAATCAAATCCACGAGTGGAAA 
               
               
                   
                   
                   
                   
                 AGCCTTTGTA 
               
               
                   
               
               
                 403 
                 QTRTD1 
                 NM_024638.3 
                  2508- 
                 TTAGATTAGAGTCATAGCCTTAA 
               
               
                   
                   
                   
                  2607 
                 TAGCCCTAGTTGTCATCCTGGGA 
               
               
                   
                   
                   
                   
                 GACAGGCAACAGTAGAGATATT 
               
               
                   
                   
                   
                   
                 TGAGAGCCTAAAGAGAGGTTTG 
               
               
                   
                   
                   
                   
                 GCCTGTGGGT 
               
               
                   
               
               
                 404 
                 RAB10 
                 NM_016131.4 
                  3593- 
                 AGGGCTTTGCCCCTTTTCTGTAA 
               
               
                   
                   
                   
                  3692 
                 GTCTCTTGGGATCCTGTGTAGAA 
               
               
                   
                   
                   
                   
                 GCTGTTCTCATTAAACACCAAAC 
               
               
                   
                   
                   
                   
                 AGTTAAGTCCATTCTCTGGTACT 
               
               
                   
                   
                   
                   
                 AGCTACAA 
               
               
                   
               
               
                 405 
                 RAG1 
                 NM_000448.2 
                  2301- 
                 CAGTCTACATTTGTACTCTTTGT 
               
               
                   
                   
                   
                  2400 
                 GATGCCACCCGTCTGGAAGCCTC 
               
               
                   
                   
                   
                   
                 TCAAAATCTTGTCTTCCACTCTA 
               
               
                   
                   
                   
                   
                 TAACCAGAAGCCATGCTGAGAAC 
               
               
                   
                   
                   
                   
                 CTGGAACG 
               
               
                   
               
               
                 406 
                 RASSF5 
                 NM_182664.2 
                  3061- 
                 TCGTCCTGCATGTCTCTAACATT 
               
               
                   
                   
                   
                  3160 
                 AATAGAAGGCATGGCTCCTGCTG 
               
               
                   
                   
                   
                   
                 CAACCGCTGTGAATGCTGCTGAG 
               
               
                   
                   
                   
                   
                 AACCTCCCTCTATGGGGATGGCT 
               
               
                   
                   
                   
                   
                 ATTTTATT 
               
               
                   
               
               
                 407 
                 RBM14 
                 NM_006328.3 
                  2661- 
                 TGGTATGTATCCAAGTCCCTGCT 
               
               
                   
                   
                   
                  2760 
                 GACCACTAATGTTCTAGCTGATG 
               
               
                   
                   
                   
                   
                 GTGAGCGGCACAGTCCCACTTCC 
               
               
                   
                   
                   
                   
                 CCATCTCCCCAAGTAGGTGGTGT 
               
               
                   
                   
                   
                   
                 TAGAAAAC 
               
               
                   
               
               
                 408 
                 RBM4B 
                 NM_031492.3 
                  1557- 
                 TAGGAGTTGAATCCTTCTCCCTG 
               
               
                   
                   
                   
                  1656 
                 CCTACCTGCAGCATCTCCTTTCC 
               
               
                   
                   
                   
                   
                 CTTTAAAATGACCATGTAGTGGC 
               
               
                   
                   
                   
                   
                 AAGCAGCCTTTTACTCTTCTGTT 
               
               
                   
                   
                   
                   
                 AGCTCTGG 
               
               
                   
               
               
                 409 
                 RBX1 
                 NM_014248.3 
                   158- 
                 GATATTGTGGTTGATAACTGTGC 
               
               
                   
                   
                   
                   257 
                 CATCTGCAGGAACCACATTATGG 
               
               
                   
                   
                   
                   
                 ATCTTTGCATAGAATGTCAAGCT 
               
               
                   
                   
                   
                   
                 AACCAGGCGTCCGCTACTTCAGA 
               
               
                   
                   
                   
                   
                 AGAGTGTA 
               
               
                   
               
               
                 410 
                 RELA 
                 NM_021975.2 
                   361- 
                 GATGGCTTCTATGAGGCTGAGCT 
               
               
                   
                   
                   
                   460 
                 CTGCCCGGACCGCTGCATCCACA 
               
               
                   
                   
                   
                   
                 GTTTCCAGAACCTGGGAATCCAG 
               
               
                   
                   
                   
                   
                 TGTGTGAAGAAGCGGGACCTGG 
               
               
                   
                   
                   
                   
                 AGCAGGCTA 
               
               
                   
               
               
                 411 
                 REPIN1 
                 NM_014374.3 
                  2491- 
                 TGTGTCCAGGCTCTTGTCTGAAC 
               
               
                   
                   
                   
                  2590 
                 ACCGCAGCCCCTCCTTCGCTCCT 
               
               
                   
                   
                   
                   
                 TCCAGAGCTCAGCATGTCACGGC 
               
               
                   
                   
                   
                   
                 AAGGACTGCCGCATTGGTGATGG 
               
               
                   
                   
                   
                   
                 AGGGCCAG 
               
               
                   
               
               
                 412 
                 REPS1 
                 NM_00112861 
                  1289- 
                 CACCAACCAGTACTCTTTTAACC 
               
               
                   
                   
                 7.2 
                  1388 
                 ATGCATCCTGCTTCTGTCCAGGA 
               
               
                   
                   
                   
                   
                 CCAGACAACAGTACGAACTGTA 
               
               
                   
                   
                   
                   
                 GCATCAGCTACAACTGCCATTGA 
               
               
                   
                   
                   
                   
                 AATTCGTAG 
               
               
                   
               
               
                 413 
                 RERE 
                 NM_00104268 
                  5916- 
                 AACCCTCGACCCGAAACCCTCAC 
               
               
                   
                   
                 2.1 
                  6015 
                 CAGATAAACTACAGTTTGTTTAG 
               
               
                   
                   
                   
                   
                 GAGGCCCTGACCTTCATGGTGTC 
               
               
                   
                   
                   
                   
                 TTTGAAGCCCAACCACTCGGTTT 
               
               
                   
                   
                   
                   
                 CCTTCGGA 
               
               
                   
               
               
                 414 
                 RERE  
                 NM_012102.3 
                  7734- 
                 GCATTCTTGTTAGCTTTGCTTTT 
               
               
                   
                 b 
                   
                  7833 
                 CTCCCCATATCCCAAGGCGAAGC 
               
               
                   
                   
                   
                   
                 GCTGAGATTCTTCCATCTAAAAA 
               
               
                   
                   
                   
                   
                 ACCCTCGACCCGAAACCCTCACC 
               
               
                   
                   
                   
                   
                 AGATAAAC 
               
               
                   
               
               
                 415 
                 RFWD2 
                 NM_022457.6 
                  2606- 
                 TTTTCTTTTCCCTCCTTTATGAC 
               
               
                   
                   
                   
                  2705 
                 CTTTGGGACATTGGGAATACCCA 
               
               
                   
                   
                   
                   
                 GCCAACTCTCCACCATCAATGTA 
               
               
                   
                   
                   
                   
                 ACTCCATGGACATTGCTGCTCTT 
               
               
                   
                   
                   
                   
                 GGTGGTGT 
               
               
                   
               
               
                 416 
                 RFX1 
                 NM_002918.4 
                  4187- 
                 ATAAAAATCACTATTTTGTGTGC 
               
               
                   
                   
                   
                  4286 
                 TCCGCGTGCTATAGCTTTTGGGG 
               
               
                   
                   
                   
                   
                 CGGCCCTGCCCAGTCCCCGTGCC 
               
               
                   
                   
                   
                   
                 CACGGGGCTCCCTCTCCCGGTGG 
               
               
                   
                   
                   
                   
                 TGAAAGTG 
               
               
                   
               
               
                 417 
                 RHOB 
                 NM_004040.3 
                  1707- 
                 GGGAGGAGGGAGGATGCGCTGT 
               
               
                   
                   
                   
                  1806 
                 GGGGTTGTTTTTGCCATAAGCGA 
               
               
                   
                   
                   
                   
                 ACTTTGTGCCTGTCCTAGAAGTG 
               
               
                   
                   
                   
                   
                 AAAATTGTTCAGTCCAAGAAACT 
               
               
                   
                   
                   
                   
                 GATGTTATT 
               
               
                   
               
               
                 418 
                 RHOG 
                 NM_001665.3 
                  1045- 
                 CTTTCCACACAGTTGTTGCTGCC 
               
               
                   
                   
                   
                  1144 
                 TATTGTGGTGCCGCCTCAGGTTA 
               
               
                   
                   
                   
                   
                 GGGGCTCTCAGCCATCTCTAACC 
               
               
                   
                   
                   
                   
                 TCTGCCCTCGCTGCTCTTGGAAT 
               
               
                   
                   
                   
                   
                 TGCGCCCC 
               
               
                   
               
               
                 419 
                 RHOU 
                 NM_021205.5 
                  4174- 
                 TTGACAGACTCAAGAGAAACTA 
               
               
                   
                   
                   
                  4273 
                 CCCAGGTATTACACAAGCCAAA 
               
               
                   
                   
                   
                   
                 ATGGGAGCAAGGCCTTCTCTCCA 
               
               
                   
                   
                   
                   
                 GACTATCGTAACCTGGTGCCTTA 
               
               
                   
                   
                   
                   
                 CCAAGTTGTG 
               
               
                   
               
               
                 420 
                 RNASE2 
                 NM_002934.2 
                   331- 
                 TGACCTGTCCTAGTAACAAAACT 
               
               
                   
                   
                   
                   430 
                 CGCAAAAATTGTCACCACAGTGG 
               
               
                   
                   
                   
                   
                 AAGCCAGGTGCCTTTAATCCACT 
               
               
                   
                   
                   
                   
                 GTAACCTCACAACTCCAAGTCCA 
               
               
                   
                   
                   
                   
                 CAGAATAT 
               
               
                   
               
               
                 421 
                 RNF114 
                 NM_018683.3 
                  2246- 
                 AATTCAGATCATCTCAGAAGTCT 
               
               
                   
                   
                   
                  2345 
                 GGAGGGAAATCTGGCGAAACCT 
               
               
                   
                   
                   
                   
                 TCGTTTGAGGGACTGATGTGAGT 
               
               
                   
                   
                   
                   
                 GTATGTCCACCTCACTGGTGGCA 
               
               
                   
                   
                   
                   
                 CCGAGAAAC 
               
               
                   
               
               
                 422 
                 RNF19B 
                 NM_153341.3 
                  2222- 
                 CCCCAGAGCCCAAGGTGCACCG 
               
               
                   
                   
                   
                  2321 
                 AGCCCAAGTGCCCATATGAACCT 
               
               
                   
                   
                   
                   
                 CTCTGCCCTAGCCGAGGGACAAA 
               
               
                   
                   
                   
                   
                 CTGTCTTGAAGCCAGAAGGTGGA 
               
               
                   
                   
                   
                   
                 GAAGCCAGA 
               
               
                   
               
               
                 423 
                 RNF214 
                 NM_207343.3 
                  2068- 
                 ACCTGTAAGCTATGTCTAATGTG 
               
               
                   
                   
                   
                  2167 
                 CCAGAAACTCGTCCAGCCCAGTG 
               
               
                   
                   
                   
                   
                 AGCTGCATCCAATGGCGTGTACC 
               
               
                   
                   
                   
                   
                 CATGTATTGCACAAGGAGTGTAT 
               
               
                   
                   
                   
                   
                 CAAATTCT 
               
               
                   
               
               
                 424 
                 RNF34 
                 NM_025126.3 
                  1619- 
                 CTTCTGTCCTCTTTGGATGAGAT 
               
               
                   
                   
                   
                  1718 
                 CAGTGTCCACAAGTGGCCGACAT 
               
               
                   
                   
                   
                   
                 GGAACATGCTGAGCAGTGGCTCC 
               
               
                   
                   
                   
                   
                 TCTGAATGTTCACTTTATTAGTC 
               
               
                   
                   
                   
                   
                 ATGTATAT 
               
               
                   
               
               
                 425 
                 C20orf 
                 NM_080748.2 
                   274- 
                 CTCAGGATCGGAATGCGGGGTC 
               
               
                   
                 52 
                   
                   373 
                 GAGAGCTGATGGGCGGCATTGG 
               
               
                   
                   
                   
                   
                 GAAAACCATGATGCAGAGTGGC 
               
               
                   
                   
                   
                   
                 GGCACCTTTGGCACATTCATGGC 
               
               
                   
                   
                   
                   
                 CATTGGGATGG 
               
               
                   
               
               
                 426 
                 RPL26 
                 NM_016093.2 
                     4- 
                 CACTCAGGGTCTGAGGCAGCTAG 
               
               
                   
                 L1 
                   
                   103 
                 TAGCCGGAGGGTCACCATGAAG 
               
               
                   
                   
                   
                   
                 TTCAATCCCTTCGTTACCTCGGA 
               
               
                   
                   
                   
                   
                 CCGCAGTAAAAACCGCAAACGT 
               
               
                   
                   
                   
                   
                 CACTTCAATG 
               
               
                   
               
               
                 427 
                 RPL3 
                 NM_00103385 
                  1072- 
                 AGAAGAAAGCATTCATGGGACC 
               
               
                   
                   
                 3.1 
                  1171 
                 ACTGAAGAAAGACCGAATTGCA 
               
               
                   
                   
                   
                   
                 AAGGAAGAAGGAGCTTAATGCC 
               
               
                   
                   
                   
                   
                 AGGAACAGATTTTGCAGTTGGTG 
               
               
                   
                   
                   
                   
                 GGGTCTCAATA 
               
               
                   
               
               
                 428 
                 RPL31 
                 NM_000993.4 
                    20- 
                 CTTGCAACTGCGGCTTTCCTTCT 
               
               
                   
                   
                   
                   119 
                 CCCACAATCCTTCGCGCTCTTCC 
               
               
                   
                   
                   
                   
                 TTTCCAACTTGGACGCTGCAGAA 
               
               
                   
                   
                   
                   
                 TGGCTCCCGCAAAGAAGGGTGGC 
               
               
                   
                   
                   
                   
                 GAGAAGAA 
               
               
                   
               
               
                 429 
                 RPL34 
                 NM_000995.3 
                   471- 
                 ACCTCACCTCAGCTTGAGAGAGC 
               
               
                   
                   
                   
                   570 
                 CAGTTGTGTGCATCTCTTTCCAG 
               
               
                   
                   
                   
                   
                 TTTTGCATCCAGTGACGTCTGCT 
               
               
                   
                   
                   
                   
                 TGGCATCTTGAGATTGTTATGGT 
               
               
                   
                   
                   
                   
                 GAGAGTAT 
               
               
                   
               
               
                 430 
                 RPL39L 
                 NM_052969.1 
                   139- 
                 GCGGGTTCGGGTCGGTGACACGC 
               
               
                   
                   
                   
                   238 
                 AGACCTGAGGGAGCTGGGCCCG 
               
               
                   
                   
                   
                   
                 CCTTTTCCGCCCGCGCCCCAGGC 
               
               
                   
                   
                   
                   
                 CCTTGCAGATCGAGATTTGCGTC 
               
               
                   
                   
                   
                   
                 CTAGAGTGG 
               
               
                   
               
               
                 431 
                 KIAA0 
                 NM_015203.4 
                  4795- 
                 CCCCTTGGGTCCCTCACACAGAG 
               
               
                   
                 460 
                   
                  4894 
                 ACACCATCAGCCGGAGTGGTATA 
               
               
                   
                   
                   
                   
                 ATCTTACGGAGTCCCCGGCCAGA 
               
               
                   
                   
                   
                   
                 CTTTCGGCCTAGGGAACCTTTTC 
               
               
                   
                   
                   
                   
                 TCAGCAGA 
               
               
                   
               
               
                 432 
                 RPS24 
                 NM_001026.4 
                   482- 
                 ATGAAGAAAGTCAGGGGGACTG 
               
               
                   
                   
                   
                   581 
                 CAAAGGCCAATGTTGGTGCTGGC 
               
               
                   
                   
                   
                   
                 AAAAAGCCGAAGGAGTAAAGGT 
               
               
                   
                   
                   
                   
                 GCTGCAATGATGTTAGCTGTGGC 
               
               
                   
                   
                   
                   
                 CACTGTGGAT 
               
               
                   
               
               
                 433 
                 RPS27L 
                 NM_015920.3 
                   241- 
                 TAAAATGTCCAGGTTGCTACAAG 
               
               
                   
                   
                   
                   340 
                 ATCACCACGGTTTTCAGCCATGC 
               
               
                   
                   
                   
                   
                 TCAGACAGTGGTTCTTTGTGTAG 
               
               
                   
                   
                   
                   
                 GTTGTTCAACAGTGTTGTGCCAG 
               
               
                   
                   
                   
                   
                 CCTACAGG 
               
               
                   
               
               
                 434 
                 RPS6 
                 NM_001010.2 
                   172- 
                 GAATGGAAGGGTTATGTGGTCCG 
               
               
                   
                   
                   
                   271 
                 AATCAGTGGTGGGAACGACAAA 
               
               
                   
                   
                   
                   
                 CAAGGTTTCCCCATGAAGCAGGG 
               
               
                   
                   
                   
                   
                 TGTCTTGACCCATGGCCGTGTCC 
               
               
                   
                   
                   
                   
                 GCCTGCTAC 
               
               
                   
               
               
                 435 
                 RSL24D 
                 NM_016304.2 
                  1232- 
                 TGGAGTGACACTACACTCTAGAA 
               
               
                   
                 1 
                   
                  1331 
                 TTTCCACTTTGGAGAATACTCAG 
               
               
                   
                   
                   
                   
                 TTCCAACTTGTGATTCCTGATAG 
               
               
                   
                   
                   
                   
                 AACAGACTTTACTTTTCTAGCCC 
               
               
                   
                   
                   
                   
                 AGCATTGA 
               
               
                   
               
               
                 436 
                 RWDD1 
                 NM_00100746 
                   998- 
                 TGGAGGATGATGAAGATGATCC 
               
               
                   
                   
                 4.2 
                  1097 
                 AGACTATAATCCTGCTGACCCAG 
               
               
                   
                   
                   
                   
                 AGAGTGACTCAGCTGACTAATGG 
               
               
                   
                   
                   
                   
                 ACTGTCCCCATCTGCAGAGAGGC 
               
               
                   
                   
                   
                   
                 TTGACTGCC 
               
               
                   
               
               
                 437 
                 RXRA 
                 NM_002957.5 
                  5301- 
                 AGTAATTTTTAAAGCCTTGCTCT 
               
               
                   
                   
                   
                  5400 
                 GTTGTGTCCTGTTGCCGGCTCTG 
               
               
                   
                   
                   
                   
                 GCCTTCCTGTGACTGACTGTGAA 
               
               
                   
                   
                   
                   
                 GTGGCTTCTCCGTACGATTGTCT 
               
               
                   
                   
                   
                   
                 CTGAAACA 
               
               
                   
               
               
                 438 
                 S100A  
                 NM_005621.1 
                   261- 
                 CAAGATGAACAGGTCGACTTTCA 
               
               
                   
                 12 b 
                   
                   360 
                 AGAATTCATATCCCTGGTAGCCA 
               
               
                   
                   
                   
                   
                 TTGCGCTGAAGGCTGCCCATTAC 
               
               
                   
                   
                   
                   
                 CACACCCACAAAGAGTAGGTAG 
               
               
                   
                   
                   
                   
                 CTCTCTGAA 
               
               
                   
               
               
                 439 
                 S100A8 
                 NM_002964.4 
                   366- 
                 GTTAACTTCCAGGAGTTCCTCAT 
               
               
                   
                   
                   
                   465 
                 TCTGGTGATAAAGATGGGCGTGG 
               
               
                   
                   
                   
                   
                 CAGCCCACAAAAAAAGCCATGA 
               
               
                   
                   
                   
                   
                 AGAAAGCCACAAAGAGTAGCTG 
               
               
                   
                   
                   
                   
                 AGTTACTGGG 
               
               
                   
               
               
                 440 
                 SAMSN1 
                 NM_022136.3 
                  1024- 
                 ACCTGAGCCCCTATCCTTGAGCT 
               
               
                   
                   
                   
                  1123 
                 CAGACATCTCCTTAAATAAGTCA 
               
               
                   
                   
                   
                   
                 CAGTTAGATGACTGCCCAAGGG 
               
               
                   
                   
                   
                   
                 ACTCTGGTTGCTATATCTCATCA 
               
               
                   
                   
                   
                   
                 GGAAATTCA 
               
               
                   
               
               
                 441 
                 SAP130  
                 NM_024545.3 
                  3091- 
                 GATCTCCACCGAATAAACGAACT 
               
               
                   
                 b 
                   
                  3190 
                 GATACAGGGAAATATGCAGAGG 
               
               
                   
                   
                   
                   
                 TGTAAACTTGTGATGGATCAAAT 
               
               
                   
                   
                   
                   
                 CAGTGAAGCCAGAGACTCCATG 
               
               
                   
                   
                   
                   
                 CTTAAGGTTT 
               
               
                   
               
               
                 442 
                 SAP130 
                 NM_024545.3 
                  3720- 
                 CGGTTCTTCTGCCTGACCTTCAA 
               
               
                   
                   
                   
                  3819 
                 ATGCCCATGTTGGCCTTTTACAG 
               
               
                   
                   
                   
                   
                 CAGTGCCACGGCACCAAGCGAG 
               
               
                   
                   
                   
                   
                 CTGCCACATCTCACACTCTAAAG 
               
               
                   
                   
                   
                   
                 GGTTTGAAC 
               
               
                   
               
               
                 443 
                 CIP29 
                 NM_033082.3 
                   622- 
                 AACTGGAACCACAGAGGATACA 
               
               
                   
                   
                   
                   721 
                 GAGGCAAAGAAGAGGAAAAGAG 
               
               
                   
                   
                   
                   
                 CAGAGCGCTTTGGGATTGCCTGA 
               
               
                   
                   
                   
                   
                 TGAAAAGTTCCTGATACTTTCTG 
               
               
                   
                   
                   
                   
                 TTCTCCAGTG 
               
               
                   
               
               
                 444 
                 SFRS 
                 NM_004719.2 
                  4203- 
                 AGTTCTTCTCATGTAAGTAATAA 
               
               
                   
                 2IP 
                   
                  4302 
                 CATGAGTACACCAGTTTTGCCTG 
               
               
                   
                   
                   
                   
                 CTCCGACAGCAGCCCCAGGAAA 
               
               
                   
                   
                   
                   
                 TACGGGAATGGTTCAGGGACCA 
               
               
                   
                   
                   
                   
                 AGTTCTGGTA 
               
               
                   
               
               
                 445 
                 SFRS15 
                 NM_020706.2 
                  3635- 
                 GAGAGAAGGAAGAAGCCCGAGG 
               
               
                   
                   
                   
                  3734 
                 AAAGGAAAAGCCTGAGGTGACA 
               
               
                   
                   
                   
                   
                 GACAGGGCAGGTGGTAACAAAA 
               
               
                   
                   
                   
                   
                 CCGTTGAACCTCCCATTAGCCAA 
               
               
                   
                   
                   
                   
                 GTGGGAAATGT 
               
               
                   
               
               
                 446 
                 RBM16 
                 NM_014892.4 
                  4111- 
                 TGATTATTTTGAAGGGGCCACTT 
               
               
                   
                   
                   
                  4210 
                 CTCAACGAAAAGGTGATAATGT 
               
               
                   
                   
                   
                   
                 GCCTCAGGTTAATGGTGAAAATA 
               
               
                   
                   
                   
                   
                 CAGAGAGACATGCTCAGCCACC 
               
               
                   
                   
                   
                   
                 ACCTATACCA 
               
               
                   
               
               
                 447 
                 SDHA 
                 NM_004168.3 
                  2042- 
                 GTCACTCTGGAATATAGACCCGT 
               
               
                   
                   
                   
                  2141 
                 GATCGACAAAACTTTGAACGAG 
               
               
                   
                   
                   
                   
                 GCTGACTGTGCCACCGTCCCGCC 
               
               
                   
                   
                   
                   
                 AGCCATTCGCTCCTACTGATGAG 
               
               
                   
                   
                   
                   
                 ACAAGATGT 
               
               
                   
               
               
                 448 
                 SEC24C 
                 NM_198597.2 
                  4194- 
                 AGGCAGAGGCAGCTGGAGCGCC 
               
               
                   
                   
                   
                  4293 
                 GTTCTCTCCTGCTGGGACACCGC 
               
               
                   
                   
                   
                   
                 TTGGGCTTTGGTATTGACTGAGT 
               
               
                   
                   
                   
                   
                 GGCTGACAGTTATCTTCCAACCC 
               
               
                   
                   
                   
                   
                 CAACTGGCT 
               
               
                   
               
               
                 449 
                 SEMG1 
                 NM_003007.2 
                  1291- 
                 GGCAGACACCAACATGGATCTC 
               
               
                   
                   
                   
                  1390 
                 ATGGGGGATTGGATATTGTAATT 
               
               
                   
                   
                   
                   
                 ATAGAGCAGGAAGATGACAGTG 
               
               
                   
                   
                   
                   
                 ATCGTCATTTGGCACAACATCTT 
               
               
                   
                   
                   
                   
                 AACAACGACC 
               
               
                   
               
               
                 450 
                 SERPIN 
                 NM_005024.1 
                   891- 
                 AGACAGTTATGATCTCAAGTCAA 
               
               
                   
                 B10 
                   
                   990 
                 CCCTGAGCAGTATGGGGATGAGT 
               
               
                   
                   
                   
                   
                 GATGCCTTCAGCCAAAGCAAAG 
               
               
                   
                   
                   
                   
                 CTGATTTCTCAGGAATGTCTTCA 
               
               
                   
                   
                   
                   
                 GCAAGAAAC 
               
               
                   
               
               
                 451 
                 SETD2 
                 NM_014159.6 
                  7956- 
                 TGGTTAGAAGCCATCAGAGGTGC 
               
               
                   
                   
                   
                  8055 
                 AAGGGCTTAGAAAAGACCCTGG 
               
               
                   
                   
                   
                   
                 CCAGACCTGACTCCACTCTTAAA 
               
               
                   
                   
                   
                   
                 CCTGGGTCTTCTCCTTGGCGGTG 
               
               
                   
                   
                   
                   
                 CTGTCAGCG 
               
               
                   
               
               
                 452 
                 SFMBT1 
                 NM_00100515 
                  2844- 
                 AAGGATCGAAGTTGCTGAAAGG 
               
               
                   
                   
                 8.2 
                  2943 
                 CTTCACCTGGACAGTAACCCCTT 
               
               
                   
                   
                   
                   
                 GAAGTGGAGTGTGGCAGACGTT 
               
               
                   
                   
                   
                   
                 GTGCGGTTCATCAGATCCACTGA 
               
               
                   
                   
                   
                   
                 CTGTGCTCCA 
               
               
                   
               
               
                 453 
                 SFPQ 
                 NM_005066.2 
                  2800- 
                 GGTTATGTAAGCAAAGCTGAACT 
               
               
                   
                   
                   
                  2899 
                 GTAAATCTTCAGGAATATGTATT 
               
               
                   
                   
                   
                   
                 AAGATTGTGGAATGGGTGTAAG 
               
               
                   
                   
                   
                   
                 ACAATTGGTAGGGGGTGAAAGT 
               
               
                   
                   
                   
                   
                 GGGTTTGATT 
               
               
                   
               
               
                 454 
                 SGK1 
                 NM _05627.3 
                  1622- 
                 ACGAGCGTTAGAGTGCCGCCTTA 
               
               
                   
                   
                   
                  1721 
                 GACGGAGGCAGGAGTTTCGTTA 
               
               
                   
                   
                   
                   
                 GAAAGCGGACGCTGTTCTAAAA 
               
               
                   
                   
                   
                   
                 AAGGTCTCCTGCAGATCTGTCTG 
               
               
                   
                   
                   
                   
                 GGCTGTGATG 
               
               
                   
               
               
                 455 
                 SGK 
                 NM_005627.3 
                   173- 
                 GAAGCAGAGGAGGATGGGTCTG 
               
               
                   
                   
                   
                   272 
                 AACGACTTTATTCAGAAGATTGC 
               
               
                   
                   
                   
                   
                 CAATAACTCCTATGCATGCAAAC 
               
               
                   
                   
                   
                   
                 ACCCTGAAGTTCAGTCCATCTTG 
               
               
                   
                   
                   
                   
                 AAGATCTCC 
               
               
                   
               
               
                 456 
                 SGK1 b 
                 NM_005627.3 
                  1814- 
                 GGATATGCTGTGTGAACCGTCGT 
               
               
                   
                   
                   
                  1913 
                 GTGAGTGTGGTATGCCTGATCAC 
               
               
                   
                   
                   
                   
                 AGATGGATTTTGTTATAAGCATC 
               
               
                   
                   
                   
                   
                 AATGTGACACTTGCAGGACACTA 
               
               
                   
                   
                   
                   
                 CAACGTGG 
               
               
                   
               
               
                 457 
                 SH2D3C 
                 NM_170600.2 
                  2795- 
                 AGCACCCCAAGGACACTGTGATC 
               
               
                   
                   
                   
                  2894 
                 AACCCGAGAATGTTCTGGGTTCA 
               
               
                   
                   
                   
                   
                 ACTCAAGCATCTCCCTTGCACCT 
               
               
                   
                   
                   
                   
                 CCAGGGTCCTGCGTGGACTCTGG 
               
               
                   
                   
                   
                   
                 GTTCCATC 
               
               
                   
               
               
                 458 
                 SIK1 
                 NM_173354.3 
                  4185- 
                 TCGCTCATAAAGAAGTTTTTGGG 
               
               
                   
                   
                   
                  4284 
                 ATGGGAGAGAATCCAGACCATC 
               
               
                   
                   
                   
                   
                 TTGGGGCAGCCAGGCCCTTGCCT 
               
               
                   
                   
                   
                   
                 TCATTTTTACAGAGGTAGCACAA 
               
               
                   
                   
                   
                   
                 CTGATTCCA 
               
               
                   
               
               
                 459 
                 SIN3A 
                 NM_015477.2 
                  4666- 
                 TTTATTCCTGACGATTCCCTTGC 
               
               
                   
                   
                   
                  4765 
                 TGCCTACCCTTTTCTCTCCTCTG 
               
               
                   
                   
                   
                   
                 GTTCTCAACCTCAACGAGTTCAA 
               
               
                   
                   
                   
                   
                 ATCAGTTGTCCTTTTTAGCTCCC 
               
               
                   
                   
                   
                   
                 GTGGAACT 
               
               
                   
               
               
                 460 
                 SLAMF8 
                 NM_020125.2 
                  3173- 
                 AACAAATATTGATTGAGGGCGCT 
               
               
                   
                   
                   
                  3272 
                 GCATGTGCTGGGTACATTTCTTG 
               
               
                   
                   
                   
                   
                 GCACTTGGGAATCAGTAGTCAAG 
               
               
                   
                   
                   
                   
                 CGAAACCCTTGCCTTTGAGAGTT 
               
               
                   
                   
                   
                   
                 TATGGTCT 
               
               
                   
               
               
                 461 
                 SLC11A 
                 NM_000578.3 
                  2072- 
                 GCAGGATAGAGTGGGACAGTTC 
               
               
                   
                 1 
                   
                  2171 
                 CTGAGACCAGCCAACCTGGGGG 
               
               
                   
                   
                   
                   
                 CTTTAGGGACCTGCTGTTTCCTA 
               
               
                   
                   
                   
                   
                 GCGCAGCCATGTGATTACCCTCT 
               
               
                   
                   
                   
                   
                 GGGTCTCAGT 
               
               
                   
               
               
                 462 
                 SLC15A 
                 NM_021082.3 
                  2548- 
                 AACTCATTAAAACTTGTGCAGTG 
               
               
                   
                 2 
                   
                  2647 
                 TTGCTGGAGCTGGCCTGGTGTCT 
               
               
                   
                   
                   
                   
                 CCAAATGACCATGAAAATACAC 
               
               
                   
                   
                   
                   
                 ACGTATAATGGAGATCATTCTCT 
               
               
                   
                   
                   
                   
                 GTGGGTATG 
               
               
                   
               
               
                 463 
                 SLC25A 
                 NM_000387.5 
                  1511- 
                 ATCTTCTTCAGTCCCTAGCCAGG 
               
               
                   
                 20 
                   
                  1610 
                 AATACCCATTTGATTTCCAGGGT 
               
               
                   
                   
                   
                   
                 GCCATCTAATCCTGGGCTGTACA 
               
               
                   
                   
                   
                   
                 TGTGGATATGGACTTGAGGCCCA 
               
               
                   
                   
                   
                   
                 CCTCTGTG 
               
               
                   
               
               
                 464 
                 SLC25A 
                 NM_016612.2 
                  1217- 
                 TCCAGCCCCTTGCCCTCTCCTCA 
               
               
                   
                 37 
                   
                  1316 
                 CACGTAGATCATTTTTTTTTTGC 
               
               
                   
                   
                   
                   
                 AGGGTGCTGCCTATGGGCCCTCT 
               
               
                   
                   
                   
                   
                 GCTCCCCAATGCCTTAGAGAGAG 
               
               
                   
                   
                   
                   
                 GAGGGGAC 
               
               
                   
               
               
                 465 
                 SLC45A 
                 NM_033102.2 
                  2455- 
                 AGTTTCTAGGATGAAACACTCCT 
               
               
                   
                 3 
                   
                  2554 
                 CCATGGGATTTGAACATATGAAA 
               
               
                   
                   
                   
                   
                 GTTATTTGTAGGGGAAGAGTCCT 
               
               
                   
                   
                   
                   
                 GAGGGGCAACACACAAGAACCA 
               
               
                   
                   
                   
                   
                 GGTCCCCTC 
               
               
                   
               
               
                 466 
                 SLC6A 
                 NM_003044.4 
                  3220- 
                 GATATTGCTAACTGATCACAGAT 
               
               
                   
                 12 
                   
                  3319 
                 TCTTTCCCACCTCACAATCCTTC 
               
               
                   
                   
                   
                   
                 CGAATGTGCTCCAGGCAGCACCA 
               
               
                   
                   
                   
                   
                 TTTGCCATCCTGCTTCTAACGCA 
               
               
                   
                   
                   
                   
                 AACCCCTG 
               
               
                   
               
               
                 467 
                 SLC6A6 
                 NM_003043.5 
                  4438- 
                 ATTCTAGACCAAAGACACAGGC 
               
               
                   
                   
                   
                  4537 
                 AGACCAAGTCCCCAGGCCCCGCC 
               
               
                   
                   
                   
                   
                 TGGAAGGAAGTCGTTCCTCAACT 
               
               
                   
                   
                   
                   
                 CTCCCCAAGGCACCTGTCTCCAA 
               
               
                   
                   
                   
                   
                 TCAGAGCCC 
               
               
                   
               
               
                 468 
                 SLC9A3 
                 NM_004252.3 
                  1811- 
                 ATTAACATGATTTTCCTGGTTGT 
               
               
                   
                 R1 
                   
                  1910 
                 TACATCCAGGGCATGGCAGTGGC 
               
               
                   
                   
                   
                   
                 CTCAGCCTTAAACTTTTGTTCCT 
               
               
                   
                   
                   
                   
                 ACTCCCACCCTCAGCGAACTGGG 
               
               
                   
                   
                   
                   
                 CAGCACGG 
               
               
                   
               
               
                 469 
                 C14orf 
                 NM_031210.5 
                    46- 
                 CGGCCTCAGCAGCGAGAGGTGC 
               
               
                   
                 156 
                   
                   145 
                 TGCGGCGCTGCGTAGAAGTATCA 
               
               
                   
                   
                   
                   
                 ATCAGCCGGTTGCTTTTGTGAGA 
               
               
                   
                   
                   
                   
                 AGAATTCCTTGGACTGCGGCGTC 
               
               
                   
                   
                   
                   
                 GAGTCAGCT 
               
               
                   
               
               
                 470 
                 SMARCC 
                 NM_003074.3 
                  5281- 
                 CAATGGCCAGGGTTTTACCTACT 
               
               
                   
                 1 
                   
                  5380 
                 TCCTGCCAGTCTTTCCCAAAGGA 
               
               
                   
                   
                   
                   
                 AACTCATTCCAAATACTTCTTTT 
               
               
                   
                   
                   
                   
                 TTCCCCTGGAGTCCGAGAAGGAA 
               
               
                   
                   
                   
                   
                 AATGGAAT 
               
               
                   
               
               
                 471 
                 SNORA 
                 NR_002984.1 
                    30- 
                 CTCGTGGGACTCTAGAGGGAGTC 
               
               
                   
                 56 
                   
                   129 
                 AGTCTGCAACAGTAAGTGGTGA 
               
               
                   
                   
                   
                   
                 GTTCTTCTGTCCAGCGTCAGTAT 
               
               
                   
                   
                   
                   
                 TTTGATGGTGGCTTTAGACTTGC 
               
               
                   
                   
                   
                   
                 CAGATAACA 
               
               
                   
               
               
                 472 
                 SNX11 
                 NM_152244.1 
                  2261- 
                 CCCTCCCTGTCGCCCACTCCTCC 
               
               
                   
                   
                   
                  2360 
                 CTCCTCTGGCTATCCTACCCTGT 
               
               
                   
                   
                   
                   
                 CTGTGGGCTCTTTTACTACCAGC 
               
               
                   
                   
                   
                   
                 CTATGCTGTGGGACTGTCATGGC 
               
               
                   
                   
                   
                   
                 ATTTAGTT 
               
               
                   
               
               
                 473 
                 SOCS1 
                 NM_003745.1 
                  1026- 
                 TTAACTGTATCTGGAGCCAGGAC 
               
               
                   
                   
                   
                  1125 
                 CTGAACTCGCACCTCCTACCTCT 
               
               
                   
                   
                   
                   
                 TCATGTTTACATATACCCAGTAT 
               
               
                   
                   
                   
                   
                 CTTTGCACAAACCAGGGGTTGGG 
               
               
                   
                   
                   
                   
                 GGAGGGTC 
               
               
                   
               
               
                 474 
                 SP2 
                 NM_003110.5 
                  2701- 
                 GGGGGCAATGATGAGCATATGA 
               
               
                   
                   
                   
                  2800 
                 ATTTTTTCTCACTCTAGCAATTC 
               
               
                   
                   
                   
                   
                 CCTTTTCTAAATGACACAGCATT 
               
               
                   
                   
                   
                   
                 TAAACTCAAATCTGGATTCAGAT 
               
               
                   
                   
                   
                   
                 AACAGCACC 
               
               
                   
               
               
                 475 
                 SPA17 
                 NM_017425.3 
                   176- 
                 CAAGGATTTGGGAATCTTCTTGA 
               
               
                   
                   
                   
                   275 
                 AGGGCTGACACGCGAGATTCTG 
               
               
                   
                   
                   
                   
                 AGAGAGCAACCGGACAATATAC 
               
               
                   
                   
                   
                   
                 CAGCTTTTGCAGCAGCCTATTTT 
               
               
                   
                   
                   
                   
                 GAGAGCCTTC 
               
               
                   
               
               
                 476 
                 SPEN 
                 NM_015001.2 
                 11995- 
                 GTATTGCCCACTCATTTGTATAA 
               
               
                   
                   
                   
                 12094 
                 GTGCGCTTCGGTACAGCACGGGT 
               
               
                   
                   
                   
                   
                 CCTGCTCCCGCGATGTGGAAGTG 
               
               
                   
                   
                   
                   
                 TCACACGGCACCTGTACAAAAA 
               
               
                   
                   
                   
                   
                 GACTGGCTA 
               
               
                   
               
               
                 477 
                 SPINK5 
                 NM_006846.3 
                  2596- 
                 GAGCAATGACAAAGAGGATCTG 
               
               
                   
                   
                   
                  2695 
                 TGTCGTGAATTTCGAAGCATGCA 
               
               
                   
                   
                   
                   
                 GAGAAATGGAAAGCTTATCTGC 
               
               
                   
                   
                   
                   
                 ACCAGAGAAAATAACCCTGTTCG 
               
               
                   
                   
                   
                   
                 AGGCCCATAT 
               
               
                   
               
               
                 478 
                 SPN 
                 NM_003123.3 
                  2346- 
                 AGTGCCTGCGTGTGTCCACTCGT 
               
               
                   
                   
                   
                  2445 
                 GGGTGTGGTTTGTGTGCAAGAGC 
               
               
                   
                   
                   
                   
                 TGAGGATTTGGCGATGCTTGGGA 
               
               
                   
                   
                   
                   
                 GGGGTAGTTGTGGGTACAGACG 
               
               
                   
                   
                   
                   
                 GTGTGGGGG 
               
               
                   
               
               
                 479 
                 SREBF1 
                 NM_00100529 
                  3985- 
                 CCCCTCCTTGCTCTGCAGGCACC 
               
               
                   
                   
                 1.2 
                  4084 
                 TTAGTGGCTTTTTTCCTCCTGTG 
               
               
                   
                   
                   
                   
                 TACAGGGAAGAGAGGGGTACATT 
               
               
                   
                   
                   
                   
                 TCCCTGTGCTGACGGAAGCCAAC 
               
               
                   
                   
                   
                   
                 TTGGCTTT 
               
               
                   
               
               
                 480 
                 SFRS4 
                 NM_005626.4 
                  2080- 
                 TACTCATGGCCCACAGTAGAATA 
               
               
                   
                   
                   
                  2179 
                 TCCAAAACGCCTTGGCTTTCAGG 
               
               
                   
                   
                   
                   
                 CCTGGCCTTTCCTACAGGGAGCT 
               
               
                   
                   
                   
                   
                 CAGTAACCTGGACGGCTCTAAGG 
               
               
                   
                   
                   
                   
                 CTGGAATG 
               
               
                   
               
               
                 481 
                 ST6GAL 
                 NM_003032.2 
                  3783- 
                 CTGATTTTAATCTTCGAATCATG 
               
               
                   
                 1 
                   
                  3882 
                 ACACTGAGTGCAGAGGAGGTGG 
               
               
                   
                   
                   
                   
                 CATTCCGACAGCAGGACATACAT 
               
               
                   
                   
                   
                   
                 GTTGGTGTGAAGACTGGGACGA 
               
               
                   
                   
                   
                   
                 CACTGGGTAG 
               
               
                   
               
               
                 482 
                 STAG3 
                 NM_012447.3 
                  3424- 
                 AAGTGCCTGCAGCATGTCTCCCA 
               
               
                   
                   
                   
                  3523 
                 GGCACCTGGCCATCCCTGGGGCC 
               
               
                   
                   
                   
                   
                 CAGTCACCACCTACTGCCACTCC 
               
               
                   
                   
                   
                   
                 CTCAGCCCTGTGGAGAACACAGC 
               
               
                   
                   
                   
                   
                 AGAGACCA 
               
               
                   
               
               
                 483 
                 STAMBP 
                 NM_006463.4 
                  1926- 
                 TTTCCTGTGGTTTATGGCAATAT 
               
               
                   
                   
                   
                  2025 
                 GAATGGAGCTTATTACTGGGGTG 
               
               
                   
                   
                   
                   
                 AGGGACAGCTTACTCCATTTGAC 
               
               
                   
                   
                   
                   
                 CAGATTGTTTGGCTAACACATCC 
               
               
                   
                   
                   
                   
                 CGAAGAAT 
               
               
                   
               
               
                 484 
                 STAT6 
                 NM_003153.4 
                  3725- 
                 ACTGTGCCCAAGTGGGTCCAAGT 
               
               
                   
                   
                   
                  3824 
                 GGCTGTGACATCTACGTATGGCT 
               
               
                   
                   
                   
                   
                 CCACACCTCCAATGCTGCCTGGG 
               
               
                   
                   
                   
                   
                 AGCCAGGGTGAGAGTCTGGGTC 
               
               
                   
                   
                   
                   
                 CAGGCCTGG 
               
               
                   
               
               
                 485 
                 STIP1 
                 NM_006819.2 
                  1906- 
                 CCCGGGGAAGACACAGAGACTC 
               
               
                   
                   
                   
                  2005 
                 GTACCTGCGCTGTTTGTGCCGCC 
               
               
                   
                   
                   
                   
                 GCTGCCTCTGGGCCCTCCCAGCA 
               
               
                   
                   
                   
                   
                 CACGCATGGTCTCTTCACCGCTG 
               
               
                   
                   
                   
                   
                 CCCTCGAGT 
               
               
                   
               
               
                 486 
                 STK16 
                 NM_003691.2 
                  1420- 
                 GGGGTAGCGGGGTCAGGACAAT 
               
               
                   
                   
                   
                  1519 
                 CATCTCAGTCCTGCATCTTTTCT 
               
               
                   
                   
                   
                   
                 TCTGCTTTCTTCCCTCCAAGAGC 
               
               
                   
                   
                   
                   
                 AAAACCTGGGCAAGGGGACTTAC 
               
               
                   
                   
                   
                   
                 TGAGTGGGG 
               
               
                   
               
               
                 487 
                 STK38 
                 NM_007271.3 
                  3269- 
                 TTGTCAGTGAAACTACTTTGGAT 
               
               
                   
                   
                   
                  3368 
                 TTTAACCTCTTAGAGGAAGAAAA 
               
               
                   
                   
                   
                   
                 AAGGTTAGGGAAGTGTCAACTCT 
               
               
                   
                   
                   
                   
                 GGATGAAGGTGATGTGTTTGCCT 
               
               
                   
                   
                   
                   
                 CTCAGTCT 
               
               
                   
               
               
                 488 
                 STOM 
                 NM_004099.5 
                  2953- 
                 TTCTGCCTTGTGAATTCGTAGTC 
               
               
                   
                   
                   
                  3052 
                 CAATCAGCTGAAATTAAATCACT 
               
               
                   
                   
                   
                   
                 TGGGAGGGACGCATAGAAGGAG 
               
               
                   
                   
                   
                   
                 CTCTAGGAACACAGTGCCAGTGC 
               
               
                   
                   
                   
                   
                 AGAAGTTTC 
               
               
                   
               
               
                 489 
                 SYNJ1 
                 NM_003895.3 
                  4746- 
                 CCCTCTGCTCCCGCCCGGCACCA 
               
               
                   
                   
                   
                  4845 
                 GCCCTCCAGTAGATCCTTTCACG 
               
               
                   
                   
                   
                   
                 ACCTTGGCCTCTAAGGCTTCACC 
               
               
                   
                   
                   
                   
                 CACACTGGACTTTACAGAAAGAT 
               
               
                   
                   
                   
                   
                 AACGCCAT 
               
               
                   
               
               
                 490 
                 TAPBP 
                 NM_003190.4 
                  3397- 
                 CTTGCCCTCCCTGGGTCGCAGAC 
               
               
                   
                   
                   
                  3496 
                 GAGGTCGGCCTCGTCATTCCCCG 
               
               
                   
                   
                   
                   
                 CAGACCGCCGCGCGTCCCTCTTG 
               
               
                   
                   
                   
                   
                 TGCGGTTCACCACAGTTGTATTT 
               
               
                   
                   
                   
                   
                 AAGTGATC 
               
               
                   
               
               
                 491 
                 TAX1BP 
                 NM_00107986 
                  2081- 
                 CAGCCAGCCTGCTCGAAACTTTA 
               
               
                   
                 1 
                 4.2 
                  2180 
                 GTCGGCCTGATGGCTTAGAGGAC 
               
               
                   
                   
                   
                   
                 TCTGAGGATAGCAAAGAAGATG 
               
               
                   
                   
                   
                   
                 AGAATGTGCCTACTGCTCCTGAT 
               
               
                   
                   
                   
                   
                 CCTCCAAGT 
               
               
                   
               
               
                 492 
                 TBC1D 
                 NM_015188.1 
                  5451- 
                 TTCCAAGGAATGCACTAAGCCTT 
               
               
                   
                 12 
                   
                  5550 
                 CAGTCTTTTTAGACTGACAGTAC 
               
               
                   
                   
                   
                   
                 TGGCAGCTAAAATATTGTACTGT 
               
               
                   
                   
                   
                   
                 ATCTTCTCTTGAGCCCAGTATGT 
               
               
                   
                   
                   
                   
                 AGGAAATA 
               
               
                   
               
               
                 493 
                 TBCE 
                 NM_00107951 
                  1541- 
                 TATGCTGAAAAACCAGCTACTAA 
               
               
                   
                   
                 5.2 
                  1640 
                 CACTGAAGATAAAATACCCTCAT 
               
               
                   
                   
                   
                   
                 CAACTTGATCAGAAAGTCCTGGA 
               
               
                   
                   
                   
                   
                 GAAACAACTGCCGGGCTCCATG 
               
               
                   
                   
                   
                   
                 ACAATTCAA 
               
               
                   
               
               
                 494 
                 TBK1 
                 NM_013254.2 
                  1611- 
                 ACCAGTCTTCAGGATATCGACAG 
               
               
                   
                   
                   
                  1710 
                 CAGATTATCTCCAGGTGGATCAC 
               
               
                   
                   
                   
                   
                 TGGCAGACGCATGGGCACATCA 
               
               
                   
                   
                   
                   
                 AGAAGGCACTCATCCGAAAGAC 
               
               
                   
                   
                   
                   
                 AGAAATGTAG 
               
               
                   
               
               
                 495 
                 TBP 
                 NM_003194.4 
                  1441- 
                 TGTAAGTGCCCACCGCGGGATGC 
               
               
                   
                   
                   
                  1540 
                 CGGGAAGGGGCATTATTTGTGCA 
               
               
                   
                   
                   
                   
                 CTGAGAACACCGCGCAGCGTGA 
               
               
                   
                   
                   
                   
                 CTGTGAGTTGCTCATACCGTGCT 
               
               
                   
                   
                   
                   
                 GCTATCTGG 
               
               
                   
               
               
                 496 
                 TCF20 
                 NM_181492.2 
                  6765- 
                 CCAGGCCTGTGTTGCCAGAGCTG 
               
               
                   
                   
                   
                  6864 
                 GCAGTGTGAGCTGTAGGCAGGG 
               
               
                   
                   
                   
                   
                 ACGGGGAGGGACTGTCGCTGTG 
               
               
                   
                   
                   
                   
                 ATCAGAGTGGGTTAAGCTGACCA 
               
               
                   
                   
                   
                   
                 GGAACACCCA 
               
               
                   
               
               
                 497 
                 TCF7L2 
                 NM_030756.4 
                  2067- 
                 GGCCCACCTGTCCATGATGCCTC 
               
               
                   
                   
                   
                  2166 
                 CGCCACCCGCCCTCCTGCTCGCT 
               
               
                   
                   
                   
                   
                 GAGGCCACCCACAAGGCCTCCG 
               
               
                   
                   
                   
                   
                 CCCTCTGTCCCAACGGGGCCCTG 
               
               
                   
                   
                   
                   
                 GACCTGCCC 
               
               
                   
               
               
                 498 
                 TCP1 
                 NM_030752.2 
                   254- 
                 GTGTTCGGTGACCGCAGCACTGG 
               
               
                   
                   
                   
                   353 
                 GGAAACGATCCGCTCCCAAAAC 
               
               
                   
                   
                   
                   
                 GTTATGGCTGCAGCTTCGATTGC 
               
               
                   
                   
                   
                   
                 CAATATTGTAAAAAGTTCTCTTG 
               
               
                   
                   
                   
                   
                 GTCCAGTTG 
               
               
                   
               
               
                 499 
                 TFCP2 
                 NM_005653.4 
                  2271- 
                 CCTCTGAAAACGGCCCTCTTGAA 
               
               
                   
                   
                   
                  2370 
                 GGGGGATATGAATGGAGATTTG 
               
               
                   
                   
                   
                   
                 AAGGTCTGCAAGAACCTGACTCG 
               
               
                   
                   
                   
                   
                 TCTGACTGTGTGTGGAGGAGTCC 
               
               
                   
                   
                   
                   
                 AGGCCATGG 
               
               
                   
               
               
                 500 
                 TGIF1 
                 NM_003244.2 
                  1041- 
                 ACCTCAACCAGGACTTCAGTGGA 
               
               
                   
                   
                   
                  1140 
                 TTTCAGCTTCTAGTGGATGTTGC 
               
               
                   
                   
                   
                   
                 ACTCAAACGGGCTGCAGAGATG 
               
               
                   
                   
                   
                   
                 GAGCTTCAGGCAAAACTTACAGC 
               
               
                   
                   
                   
                   
                 TTAACCCAT 
               
               
                   
               
               
                 501 
                 TGIF1  
                 NM_173208.1 
                   691- 
                 CCCCGGGATCAGTTTTGGCTCGT 
               
               
                   
                 b 
                   
                   790 
                 CCATCAGTGATCTGCCATACCAC 
               
               
                   
                   
                   
                   
                 TGTGACTGCATTGAAAGATGTCC 
               
               
                   
                   
                   
                   
                 CTTTCTCTCTCTGCCAGTCGGTC 
               
               
                   
                   
                   
                   
                 GGTGTGGG 
               
               
                   
               
               
                 502 
                 TIAM1 
                 NM_003253.2 
                  5293- 
                 CCTAACTCTGCCCACCCTCCTGT 
               
               
                   
                   
                   
                  5392 
                 ACCGTCGACAAGAATGTCCCCTT 
               
               
                   
                   
                   
                   
                 AGGTCGCGCTCTTGCACACACGG 
               
               
                   
                   
                   
                   
                 TTTTGGCAGCTGACTTGGTTCTG 
               
               
                   
                   
                   
                   
                 AAGCCATG 
               
               
                   
               
               
                 503 
                 TIMM8B 
                 ENST0000050 
                   339- 
                 GAATGACAGAAGCAAAGGACTT 
               
               
                   
                   
                 4148.1 
                   438 
                 GTTACTAAGCAGATTTAAGGGTC 
               
               
                   
                   
                   
                   
                 AGTGGGGGAAGGCTATCAACCC 
               
               
                   
                   
                   
                   
                 ATTGTCAGATCAGCATCAGGCTG 
               
               
                   
                   
                   
                   
                 TTATCAAGTC 
               
               
                   
               
               
                 504 
                 TM2D2 
                 NM_078473.2 
                  2970- 
                 ACCCATCATCCATCTGCCCACAA 
               
               
                   
                   
                   
                  3069 
                 ACCTGGCCAAATGTGATACAACC 
               
               
                   
                   
                   
                   
                 TGAAAACCTGATGGACTAAAGG 
               
               
                   
                   
                   
                   
                 AGTACTATTTAACAATTGATTGC 
               
               
                   
                   
                   
                   
                 CTTTGCACT 
               
               
                   
               
               
                 505 
                 TM9SF1 
                 NM_006405.6 
                  1996- 
                 CGCTGGTGGTGGCGATCTGTGCT 
               
               
                   
                   
                   
                  2095 
                 GAGTGTTGGCTCCACCGGCCTCT 
               
               
                   
                   
                   
                   
                 TCATCTTCCTCTACTCAGTTTTC 
               
               
                   
                   
                   
                   
                 TATTATGCCCGGCGCTCCAACAT 
               
               
                   
                   
                   
                   
                 GTCTGGGG 
               
               
                   
               
               
                 506 
                 CCDC72 
                 NM_015933.4 
                   124- 
                 GAGGAGCAGAAGAAACTCGAGG 
               
               
                   
                   
                   
                   223 
                 AGCTAAAAGCGAAGGCCGCGGG 
               
               
                   
                   
                   
                   
                 GAAGGGGCCCTTGGCCACAGGT 
               
               
                   
                   
                   
                   
                 GGAATTAAGAAATCTGGCAAAA 
               
               
                   
                   
                   
                   
                 AGTAAGCTGTTC 
               
               
                   
               
               
                 507 
                 TMBIM6 
                 NM_003217.2 
                  2282- 
                 CTCTCCCTATTCACAACCAGTGC 
               
               
                   
                   
                   
                  2381 
                 ACAGTTTGACACAGTGGCCTCAG 
               
               
                   
                   
                   
                   
                 GTTCACAGTGCACCATGTCACTG 
               
               
                   
                   
                   
                   
                 TGCTATCCTACGAAATCATTTGT 
               
               
                   
                   
                   
                   
                 TTCTAAGT 
               
               
                   
               
               
                 508 
                 TMC8 
                 NM_152468.4 
                  2238- 
                 AGGCCAATGCCAGGGCCATCCA 
               
               
                   
                   
                   
                  2337 
                 CAGGCTCCGGAAGCAGCTGGTGT 
               
               
                   
                   
                   
                   
                 GGCAGGTTCAGGAGAAGTGGCA 
               
               
                   
                   
                   
                   
                 CCTGGTGGAGGACCTGTCGCGAC 
               
               
                   
                   
                   
                   
                 TGCTGCCGGA 
               
               
                   
               
               
                 509 
                 TMCO1 
                 NM_019026.3 
                   992- 
                 TCATTTACATAAGTATTTTCTGT 
               
               
                   
                   
                   
                  1091 
                 GGGACCGACTCTCAAGGCACTGT 
               
               
                   
                   
                   
                   
                 GTATGCCCTGCAAGTTGGCTGTC 
               
               
                   
                   
                   
                   
                 TATGAGCATTTAGAGATTTAGAA 
               
               
                   
                   
                   
                   
                 GAAAAATT 
               
               
                   
               
               
                 510 
                 TMEM 
                 NM_00110082 
                  7652- 
                 AGGAGAATAAATGTTGGAGGGG 
               
               
                   
                 170B 
                 9.2 
                  7751 
                 TAATACACAAAAACAAAGGCAT 
               
               
                   
                   
                   
                   
                 ATTTGATGAAGTACCCTGTGTTA 
               
               
                   
                   
                   
                   
                 TGTGAACACAATTTCCCCTTCTG 
               
               
                   
                   
                   
                   
                 TTAAGACTAT 
               
               
                   
               
               
                 511 
                 TMEM 
                 NM_00108054 
                  1313- 
                 GCTCTGTGAAGGCAATGAGTGTC 
               
               
                   
                 218 
                 6.2 
                  1412 
                 ACTTCCCTCTGCTCTAATAAAGC 
               
               
                   
                   
                   
                   
                 AATAAATAATAGCTAAAGGGCT 
               
               
                   
                   
                   
                   
                 GACTTTCACTTCGAACTCTTGGC 
               
               
                   
                   
                   
                   
                 CACGGCTTT 
               
               
                   
               
               
                 512 
                 TMEM70 
                 NM_017866.5 
                  1952- 
                 GGTGGTTAGCTATACGGGAAATG 
               
               
                   
                   
                   
                  2051 
                 GTAAGTAGTGTTGTCTTCAGTAT 
               
               
                   
                   
                   
                   
                 CTTAATTTGTTTCTGCAACTGTG 
               
               
                   
                   
                   
                   
                 CACTCCTCCCTTGGTGGCACCCT 
               
               
                   
                   
                   
                   
                 ATGGGTGT 
               
               
                   
               
               
                 513 
                 TMSB4X 
                 NM_021109.3 
                   286- 
                 TTAACTTTGTAAGATGCAAAGAG 
               
               
                   
                   
                   
                   385 
                 GTTGGATCAAGTTTAAATGACTG 
               
               
                   
                   
                   
                   
                 TGCTGCCCCTTTCACATCAAAGA 
               
               
                   
                   
                   
                   
                 ACTACTGACAACGAAGGCCGCG 
               
               
                   
                   
                   
                   
                 CCTGCCTTT 
               
               
                   
               
               
                 514 
                 TNFRSF 
                 NM_001561.5 
                  1848- 
                 GCCTGGAGGAAGTTTTGGAAAG 
               
               
                   
                 9 
                   
                  1947 
                 AGTTCAAGTGTCTGTATATCCTA 
               
               
                   
                   
                   
                   
                 TGGTCTTCTCCATCCTCACACCT 
               
               
                   
                   
                   
                   
                 TCTGCCTTTGTCCTGCTCCCTTT 
               
               
                   
                   
                   
                   
                 TAAGCCAGG 
               
               
                   
               
               
                 515 
                 TNFSF 
                 NM_003808.3 
                   811- 
                 AGTCAGAGAGCCGGCACTCTCA 
               
               
                   
                 13 
                   
                   910 
                 GTTGCCCTCTGGTTGAGTTGGGG 
               
               
                   
                   
                   
                   
                 GGCAGCTCTGGGGGCCGTGGCTT 
               
               
                   
                   
                   
                   
                 GTGCCATGGCTCTGCTGACCCAA 
               
               
                   
                   
                   
                   
                 CAAACAGAG 
               
               
                   
               
               
                 516 
                 TNFSF8 
                 NM_001244.3 
                   519- 
                 CCCTCAAAGGAGGAAATTGCTCA 
               
               
                   
                   
                   
                   618 
                 GAAGACCTCTTATGTATCCTGAA 
               
               
                   
                   
                   
                   
                 AAGGGCTCCATTCAAGAAGTCAT 
               
               
                   
                   
                   
                   
                 GGGCCTACCTCCAAGTGGCAAA 
               
               
                   
                   
                   
                   
                 GCATCTAAA 
               
               
                   
               
               
                 517 
                 TOMM7 
                 NM_019059.2 
                   251- 
                 TCTGGCTCGGATAAGAGATGGG 
               
               
                   
                   
                   
                   350 
                 ACATCATTCAGTCACTAGTTGGA 
               
               
                   
                   
                   
                   
                 TGGCACAAGGCTCTTCACAGACG 
               
               
                   
                   
                   
                   
                 CATCTGTAGCAGAGTGGATCTTG 
               
               
                   
                   
                   
                   
                 TACTAACTT 
               
               
                   
               
               
                 518 
                 TP53BP 
                 NM_005657.2 
                  5591- 
                 TACTTCCTGTGCCTTGCCAGTGG 
               
               
                   
                 1 
                   
                  5690 
                 GATTCCTTGTGTGTCTCATGTCT 
               
               
                   
                   
                   
                   
                 GGGTCCATGATAGTTGCCATGCC 
               
               
                   
                   
                   
                   
                 AACCAGCTCCAGAACTACCGTAA 
               
               
                   
                   
                   
                   
                 TTATCTGT 
               
               
                   
               
               
                 519 
                 TPR 
                 NM_003292.2 
                  7194- 
                 TCTCCCCTCCACCAGCCAGGATC 
               
               
                   
                   
                   
                  7293 
                 CTCCTTCTAGCTCATCTGTAGAT 
               
               
                   
                   
                   
                   
                 ACTAGTAGTAGTCAACCAAAGCC 
               
               
                   
                   
                   
                   
                 TTTCAGACGAGTAAGACTTCAGA 
               
               
                   
                   
                   
                   
                 CAACATTG 
               
               
                   
               
               
                 520 
                 TPT1 
                 NM_003295.3 
                    18- 
                 GCCTGCGTCGCTTCCGGAGGCGC 
               
               
                   
                   
                   
                   117 
                 AGCGGGCGATGACGTAGAGGGA 
               
               
                   
                   
                   
                   
                 CGTGCCCTCTATATGAGGTTGGG 
               
               
                   
                   
                   
                   
                 GAGCGGCTGAGTCGGCCTTTTCC 
               
               
                   
                   
                   
                   
                 GCCCGCTCC 
               
               
                   
               
               
                 521 
                 TRAF 
                 NM_147686.3 
                  2449- 
                 GCCAGTGTCCCATATGTTCCTCC 
               
               
                   
                 3IP2 
                   
                  2548 
                 TGACAGTTTGATGTGTCCATTCT 
               
               
                   
                   
                   
                   
                 GGGCCTCTCAGTGCTTAGCAAGT 
               
               
                   
                   
                   
                   
                 AGATAATGTAAGGGATGTGGCA 
               
               
                   
                   
                   
                   
                 GCAAATGGA 
               
               
                   
               
               
                 522 
                 TRAF6 
                 NM_145803.1 
                  1840- 
                 CACCCGCTTTGACATGGGTAGCC 
               
               
                   
                   
                   
                  1939 
                 TTCGGAGGGAGGGTTTTCAGCCA 
               
               
                   
                   
                   
                   
                 CGAAGTACTGATGCAGGGGTAT 
               
               
                   
                   
                   
                   
                 AGCTTGCCCTCACTTGCTCAAAA 
               
               
                   
                   
                   
                   
                 ACAACTACC 
               
               
                   
               
               
                 523 
                 LBA1 
                 NM_014831.2 
                 10132- 
                 CTGGGAAACCTTCATGCCTCTCT 
               
               
                   
                   
                   
                 10231 
                 GATGGTTACTGCCCACCCTTACC 
               
               
                   
                   
                   
                   
                 CCACCCCTCAGCTCAGCCTGGTA 
               
               
                   
                   
                   
                   
                 TGGAAAGCAAGGTGCACGTTGG 
               
               
                   
                   
                   
                   
                 TCTTTGATT 
               
               
                   
               
               
                 524 
                 TRIM21 
                 NM_003141.3 
                  1637-  
                 TCTGCAGAGGCATCCGGATCCCA 
               
               
                   
                   
                   
                  1736 
                 GCAAGCGAGCTTTAGCAGGGAA 
               
               
                   
                   
                   
                   
                 GTCACTTCACCATCAACATTCCT 
               
               
                   
                   
                   
                   
                 GCCCCAGATGGCTTTGTGATTCC 
               
               
                   
                   
                   
                   
                 CTCCAGTGA 
               
               
                   
               
               
                 525 
                 TRIM32 
                 NM_012210.3 
                  2681- 
                 GTGCTACCAAAGGGGATACACA 
               
               
                   
                   
                   
                  2780 
                 AGCCCTTTAGGAAGCAGTACCTC 
               
               
                   
                   
                   
                   
                 TCGCCTGGAGGATCTGTGCCATC 
               
               
                   
                   
                   
                   
                 TTGGATTGAGAATTGCAGATGTG 
               
               
                   
                   
                   
                   
                 ACAGAATGG 
               
               
                   
               
               
                 526 
                 TRIM39 
                 NM_021253.3 
                  3141- 
                 CTGCTATTCGGGTAATCTTCACA 
               
               
                   
                   
                   
                  3240 
                 GAAATGACTGAGAGAAGAATCT 
               
               
                   
                   
                   
                   
                 GCAGTTTACTGAGGGCATTTCAG 
               
               
                   
                   
                   
                   
                 TTCCTCCTACCACCTCAACAGGA 
               
               
                   
                   
                   
                   
                 CTTTGTCCA 
               
               
                   
               
               
                 527 
                 TRIM39  
                 NM_172016.2 
                  2841- 
                 CTCTATACCAATAAGTCAGTCAC 
               
               
                   
                 b 
                   
                  2940 
                 CTTGCTCCTCTCCAGAGGCAAAG 
               
               
                   
                   
                   
                   
                 TGGAAGAGATCCTGCAAGACAC 
               
               
                   
                   
                   
                   
                 ATCTATCCTTTCACAGTGTTCCC 
               
               
                   
                   
                   
                   
                 AAGGGAACT 
               
               
                   
               
               
                 528 
                 TRRAP 
                 NM_003496.3 
                 12169- 
                 AGTTGATGAACCCATCATGCTGG 
               
               
                   
                   
                   
                 12268 
                 TTTTTCTCTGAGCACAAAGTTTT 
               
               
                   
                   
                   
                   
                 AGGCTGTACACAGCCAGCCTTGG 
               
               
                   
                   
                   
                   
                 GAATCTCGTTGAGCGTTCGGCGT 
               
               
                   
                   
                   
                   
                 GGATCCAC 
               
               
                   
               
               
                 529 
                 TSC1 
                 NM_000368.4 
                  8068- 
                 CCCCAGACCAACCCTTCCCTCCC 
               
               
                   
                   
                   
                  8167 
                 TTTCCCCACCTCTTACAGTGTTT 
               
               
                   
                   
                   
                   
                 GGACAGGAGGGTATGGTGCTGCT 
               
               
                   
                   
                   
                   
                 CTGTGTAGCAAGTACTTTGGCTT 
               
               
                   
                   
                   
                   
                 ATGAAAGA 
               
               
                   
               
               
                 530 
                 TTC9 
                 NM_015351.1 
                  4050- 
                 TACTAATCAGGCATCTGACCTGC 
               
               
                   
                   
                   
                  4149 
                 ACTGTCATCCCCTGCCTGGACTT 
               
               
                   
                   
                   
                   
                 TTGCGATGGACTCTTTGGGGGAA 
               
               
                   
                   
                   
                   
                 AAACTAACGCTTTTTAATTATTG 
               
               
                   
                   
                   
                   
                 TGAAAGCA 
               
               
                   
               
               
                 531 
                 TTN 
                 NM_133378.4 
                   850- 
                 TCGACTGCTCAGATCTCAGAATC 
               
               
                   
                   
                   
                   949 
                 AAGACAAACCCGAATTGAAAAG 
               
               
                   
                   
                   
                   
                 AAGATTGAAGCCCACTTTGATGC 
               
               
                   
                   
                   
                   
                 CAGATCAATTGCAACAGTTGAGA 
               
               
                   
                   
                   
                   
                 TGGTCATAG 
               
               
                   
               
               
                 532 
                 TUBB 
                 NM_178014.2 
                  2223- 
                 CAAAAAAGAATGAACACCCCTG 
               
               
                   
                   
                   
                  2322 
                 ACTCTGGAGTGGTGTATACTGCC 
               
               
                   
                   
                   
                   
                 ACATCAGTGTTTGAGTCAGTCCC 
               
               
                   
                   
                   
                   
                 CAGAGGAGAGGGGAACCCTCCT 
               
               
                   
                   
                   
                   
                 CCATCTTTTT 
               
               
                   
               
               
                 533 
                 TUG1 
                 NR_002323.2 
                  7082- 
                 TAAGCTAGAGGTCATGGTCACTG 
               
               
                   
                   
                   
                  7181 
                 AAATTACTTTCCAAAGTGGAAGA 
               
               
                   
                   
                   
                   
                 CAAAATGAAACAGGAACTGAGG 
               
               
                   
                   
                   
                   
                 GAATATTTAAGATCCCACAGAAG 
               
               
                   
                   
                   
                   
                 CGTAAAAAT 
               
               
                   
               
               
                 534 
                 TXN 
                 NM_003329.3 
                   152- 
                 TTGGATCCATTTCCATCGGTCCT 
               
               
                   
                   
                   
                   251 
                 TACAGCCGCTCGTCAGACTCCAG 
               
               
                   
                   
                   
                   
                 CAGCCAAGATGGTGAAGCAGATC 
               
               
                   
                   
                   
                   
                 GAGAGCAAGACTGCTTTTCAGGA 
               
               
                   
                   
                   
                   
                 AGCCTTGG 
               
               
                   
               
               
                 535 
                 TXNDC 
                 NM_032731.3 
                   378- 
                 TCATCTACTGCCAAGTAGGAGAA 
               
               
                   
                 17 
                   
                   477 
                 AAGCCTTATTGGAAAGATCCAAA 
               
               
                   
                   
                   
                   
                 TAATGACTTCAGAAAAAACTTGA 
               
               
                   
                   
                   
                   
                 AAGTAACAGCAGTGCCTACACTA 
               
               
                   
                   
                   
                   
                 CTTAAGTA 
               
               
                   
               
               
                 536 
                 TXNRD1 
                 NM_00109377 
                  3348- 
                 CTCAGTTGCAGCACTGAGTGGTC 
               
               
                   
                   
                 1.2 
                  3447 
                 AAAATACATTTCTGGGCCACCTC 
               
               
                   
                   
                   
                   
                 AGGGAACCCATGCATCTGCCTGG 
               
               
                   
                   
                   
                   
                 CATTTAGGCAGCAGAGCCCCTGA 
               
               
                   
                   
                   
                   
                 CCGTCCCC 
               
               
                   
               
               
                 537 
                 TXNRD1  
                 NM_182743.2 
                  2438- 
                 TGTTGCATGGAAGGGATAGTTTG 
               
               
                   
                 b 
                   
                  2537 
                 GCTCCCTTGGAGGCTATGTAGGC 
               
               
                   
                   
                   
                   
                 TTGTCCCGGGAAAGAGAACTGTC 
               
               
                   
                   
                   
                   
                 CTGCAGCTGAAATGGACTGTTCT 
               
               
                   
                   
                   
                   
                 TTACTGAC 
               
               
                   
               
               
                 538 
                 U2AF2 
                 NM_007279.2 
                  2871- 
                 TTTATGGCCAAACTATTTTGAAT 
               
               
                   
                   
                   
                  2970 
                 TTTGTTGTCCGGCCCTCAGTGCC 
               
               
                   
                   
                   
                   
                 CTGCCCTCTCCCTTACCAGGACC 
               
               
                   
                   
                   
                   
                 ACAGCTCTGTTCCTTCGGCCTCT 
               
               
                   
                   
                   
                   
                 GGTCCTCT 
               
               
                   
               
               
                 539 
                 UBA1 
                 NM_003334.3 
                  3307- 
                 CCGCCACGTGCGGGCGCTGGTGC 
               
               
                   
                   
                   
                  3406 
                 TTGAGCTGTGCTGTAACGACGAG 
               
               
                   
                   
                   
                   
                 AGCGGCGAGGATGTCGAGGTTC 
               
               
                   
                   
                   
                   
                 CCTATGTCCGATACACCATCCGC 
               
               
                   
                   
                   
                   
                 TGACCCCGT 
               
               
                   
               
               
                 540 
                 UBC 
                 NM_021009.3 
                  1876- 
                 TGCAGATCTTCGTGAAGACCCTG 
               
               
                   
                   
                   
                  1975 
                 ACTGGTAAGACCATCACTCTCGA 
               
               
                   
                   
                   
                   
                 AGTGGAGCCGAGTGACACCATT 
               
               
                   
                   
                   
                   
                 GAGAATGTCAAGGCAAAGATCC 
               
               
                   
                   
                   
                   
                 AAGACAAGGA 
               
               
                   
               
               
                 541 
                 UBE2G1 
                 NM_003342.4 
                   685- 
                 ACGCTGGCTCCCTATCCACACTG 
               
               
                   
                   
                   
                   784 
                 TGGAAACCATCATGATTAGTGTC 
               
               
                   
                   
                   
                   
                 ATTTCTATGCTGGCAGACCCTAA 
               
               
                   
                   
                   
                   
                 TGGAGACTCACCTGCTAATGTTG 
               
               
                   
                   
                   
                   
                 ATGCTGCG 
               
               
                   
               
               
                 542 
                 UBE2I 
                 NM_194259.2 
                   288- 
                 CTGCTCTGCTGACTGGGGAAGTC 
               
               
                   
                   
                   
                   387 
                 ATCGTGCCACCCAGAACCTGAGT 
               
               
                   
                   
                   
                   
                 GCGGGCCTCTCAGAGCTCCTTCG 
               
               
                   
                   
                   
                   
                 TCCGTGGGTCTGCCGGGGACTGG 
               
               
                   
                   
                   
                   
                 GCCTTGTC 
               
               
                   
               
               
                 543 
                 UBTF 
                 NM_00107668 
                  2724- 
                 GGGGGTCCCAAAGAGTTTGATG 
               
               
                   
                   
                 3.1 
                  2823 
                 AGGCCCTCCACACCTGCGGCCCA 
               
               
                   
                   
                   
                   
                 ATCCAAGGTGGGGTGGAAGCTT 
               
               
                   
                   
                   
                   
                 GGGGAAGACCCATTCCTTCCCAG 
               
               
                   
                   
                   
                   
                 AGGGGCCTGC 
               
               
                   
               
               
                 544 
                 UQCRQ 
                 NM_014402.4 
                    97- 
                 TGACGCGGATGCGGCATGTGATC 
               
               
                   
                   
                   
                   196 
                 AGCTACAGCTTGTCACCGTTCGA 
               
               
                   
                   
                   
                   
                 GCAGCGCGCCTATCCGCACGTCT 
               
               
                   
                   
                   
                   
                 TCACTAAAGGAATCCCCAATGTT 
               
               
                   
                   
                   
                   
                 CTGCGCCG 
               
               
                   
               
               
                 545 
                 USP16 
                 NM_00103241 
                  2487- 
                 TCTATTCCTTATATGGAGTTGTT 
               
               
                   
                   
                 0.1 
                  2586 
                 GAACACAGTGGTACTATGAGGTC 
               
               
                   
                   
                   
                   
                 GGGGCATTACACTGCCTATGCCA 
               
               
                   
                   
                   
                   
                 AGGCAAGAACCGCAAATAGTCAT 
               
               
                   
                   
                   
                   
                 CTCTCTAA 
               
               
                   
               
               
                 546 
                 USP21 
                 NM_012475.4 
                  1499- 
                 CCTTTTCACTAAGGAAGAAGAGC 
               
               
                   
                   
                   
                  1598 
                 TAGAGTCGGAGAATGCCCCAGT 
               
               
                   
                   
                   
                   
                 GTGTGACCGATGTCGGCAGAAA 
               
               
                   
                   
                   
                   
                 ACTCGAAGTACCAAAAAGTTGA 
               
               
                   
                   
                   
                   
                 CAGTACAAAGA 
               
               
                   
               
               
                 547 
                 USP34 
                 NM_014709.3 
                 10104- 
                 AGGAGCACACTGTAGACAGCTG 
               
               
                   
                   
                   
                 10203 
                 CATCAGTGACATGAAAACAGAA 
               
               
                   
                   
                   
                   
                 ACCAGGGAGGTCCTGACCCCAA 
               
               
                   
                   
                   
                   
                 CGAGCACTTCTGACAATGAGACC 
               
               
                   
                   
                   
                   
                 AGAGACTCCTC 
               
               
                   
               
               
                 548 
                 USP5 
                 NM_003481.2 
                  2720- 
                 AGAGCAGAGGGGCAGCGATAGA 
               
               
                   
                   
                   
                  2819 
                 CTCTGGGGATGGAGCAGGACGG 
               
               
                   
                   
                   
                   
                 GGACGGGAGGGGCCGGCCACCT 
               
               
                   
                   
                   
                   
                 GTCTGTAAGGAGACTTTGTTGCT 
               
               
                   
                   
                   
                   
                 TCCCCTGCCCC 
               
               
                   
               
               
                 549 
                 USP9Y 
                 NM_004654.3 
                    86- 
                 GGTGTGGAAAGACTTTTCTGGGC 
               
               
                   
                   
                   
                   185 
                 TCAGAGGTGAAACTGACCCTTGT 
               
               
                   
                   
                   
                   
                 GTATCAGCAGCATTTCTGACTGA 
               
               
                   
                   
                   
                   
                 CTGAGAGAGTGTAGTGATTAACA 
               
               
                   
                   
                   
                   
                 GAGTTGTG 
               
               
                   
               
               
                 550 
                 VPS37C 
                 NM_017966.4 
                  2579- 
                 TTATAAAGAGAAATCACTAATGG 
               
               
                   
                   
                   
                  2678 
                 ACTCTACTGGTTTGAGTGCTTCT 
               
               
                   
                   
                   
                   
                 GAGCTGGATGACCGACCGCCTGT 
               
               
                   
                   
                   
                   
                 ATGTTTGTGTAATTAATTGCCAT 
               
               
                   
                   
                   
                   
                 AATAAACT 
               
               
                   
               
               
                 551 
                 WDR1 
                 NM_005112.4 
                  2325- 
                 AACTGTTGCCTGTCAGTGTTTAC 
               
               
                   
                   
                   
                  2424 
                 AAACTAGTGCGTTGACGGCACCG 
               
               
                   
                   
                   
                   
                 TGTCCAAGTTTTTAGAACCCTTG 
               
               
                   
                   
                   
                   
                 TTAGCCAGACCGAGGTGTCCTGG 
               
               
                   
                   
                   
                   
                 TCACCGTT 
               
               
                   
               
               
                 552 
                 WDR91 
                 NM_014149.3 
                  2777- 
                 CAGGCTCTCCTGTTGCTTTGCCA 
               
               
                   
                   
                   
                  2876 
                 TGGAGCCAGGTCAGCTCTCTGTC 
               
               
                   
                   
                   
                   
                 TGTTCTGCTGGGTAACAAGGTTT 
               
               
                   
                   
                   
                   
                 GGCAGTTCCTGTTTCTCTGGGCT 
               
               
                   
                   
                   
                   
                 TAAGTCAA 
               
               
                   
               
               
                 553 
                 XCL2 
                 NM_003175.3 
                   378- 
                 GTAGTCTCTGGCACCCTGTCCGT 
               
               
                   
                   
                   
                   477 
                 CTCCAGCCAGCCAGCTCATTTCA 
               
               
                   
                   
                   
                   
                 CTTTACACCCTCATGGACTGAGA 
               
               
                   
                   
                   
                   
                 TTATACTCACCTTTTATGAAAGC 
               
               
                   
                   
                   
                   
                 ACTGCATG 
               
               
                   
               
               
                 554 
                 XPC 
                 NR_027299.1 
                  3168-  
                 CTGGATGGTGGTGCATCCGTGAA 
               
               
                   
                   
                   
                  3267 
                 TGCGCTGATCGTTTCTTCCAGTT 
               
               
                   
                   
                   
                   
                 AGAGTCTTCATCTGTCCGACAAG 
               
               
                   
                   
                   
                   
                 TTCACTCGCCTCGGTTGCGGACC 
               
               
                   
                   
                   
                   
                 TAGGACCA 
               
               
                   
               
               
                 555 
                 YPEL1 
                 NM_013313.4 
                  3672- 
                 GCTCATTTTTAAACCAAATGAAC 
               
               
                   
                   
                   
                  3771 
                 AGACCATGAGCTGGCTTCAGGG 
               
               
                   
                   
                   
                   
                 GAAGTGCTATTCACAGGACCATA 
               
               
                   
                   
                   
                   
                 TCCACCACCCTCTTAAATTCCTA 
               
               
                   
                   
                   
                   
                 AACAATATC 
               
               
                   
               
               
                 556 
                 ZMIZ1 
                 NM_020338.3 
                  7171- 
                 ATGATCACAGGTGATTCACACGT 
               
               
                   
                   
                   
                  7270 
                 ACACACATAAACACACCCACCA 
               
               
                   
                   
                   
                   
                 GTGCAGCCTGAAGTAACTCCCAC 
               
               
                   
                   
                   
                   
                 AGAAACCATCATCGTCTTTGTAC 
               
               
                   
                   
                   
                   
                 ATCGTATGT 
               
               
                   
               
               
                 557 
                 ZNF143 
                 NM_003442.5 
                  2292- 
                 TATCAGATCACAAACTCCTAGAG 
               
               
                   
                   
                   
                  2391 
                 TCTACATGCAAGACTAGTAAAGT 
               
               
                   
                   
                   
                   
                 CTTATGGAGTCTTATGATGGATT 
               
               
                   
                   
                   
                   
                 TTTAACTTCCCGTGGAAAAAAAA 
               
               
                   
                   
                   
                   
                 ATAAAGGC 
               
               
                   
               
               
                 558 
                 ZNF239 
                 NM_00109928 
                  1496- 
                 AGAGCTCCAACCTTCACATCCAC 
               
               
                   
                   
                 3.1 
                  1595 
                 CAGCGGGTTCACAAGAAAGATC 
               
               
                   
                   
                   
                   
                 CTCGCTAACTGACATTAGCCCAT 
               
               
                   
                   
                   
                   
                 TCAGGTCTTCACAGCGCTCATAC 
               
               
                   
                   
                   
                   
                 TGTAAAAAC 
               
               
                   
               
               
                 559 
                 ZNF341 
                 NM_032819.4 
                  3247- 
                 CAGACGGTTCCCCACAGCATCCT 
               
               
                   
                   
                   
                  3346 
                 CAGACAGCTCTGTGATGTAGCTT 
               
               
                   
                   
                   
                   
                 TTAGGAGGCACTCAGGTGTCACG 
               
               
                   
                   
                   
                   
                 GCTAGACTGCAGCTATGAGACA 
               
               
                   
                   
                   
                   
                 GATCTGGCT 
               
               
                   
               
            
           
         
       
     
     C. Polymerase Chain Reaction (PCR) Techniques 
     Another suitable quantitative method is RT-PCR, which can be used to compare mRNA levels in different sample populations, in normal and tumor tissues, to characterize patterns of gene expression, to discriminate between closely related mRNAs, and to analyze RNA structure. The first step is the isolation of mRNA from a target sample (e.g., typically total RNA isolated from human PBMC). mRNA can be extracted, for example, from frozen or archived paraffin-embedded and fixed (e.g. formalin-fixed) tissue samples. 
     General methods for mRNA extraction are well known in the art, such standard textbooks of molecular biology. In particular, RNA isolation can be performed using a purification kit, buffer set and protease from commercial manufacturers, according to the manufacturer&#39;s instructions. Exemplary commercial products include TRI-REAGENT, Qiagen RNeasy mini-columns, MASTERPURE Complete DNA and RNA Purification Kit (EPICENTRE®, Madison, Wis.), Paraffin Block RNA Isolation Kit (Ambion, Inc.) and RNA Stat-60 (Tel-Test). Conventional techniques such as cesium chloride density gradient centrifugation may also be employed. 
     The first step in gene expression profiling by RT-PCR is the reverse transcription of the RNA template into cDNA, followed by its exponential amplification in a PCR reaction. The two most commonly used reverse transcriptases are avilo myeloblastosis virus reverse transcriptase (AMV-RT) and Moloney murine leukemia virus reverse transcriptase (MMLV-RT). The reverse transcription step is typically primed using specific primers, random hexamers, or oligo-dT primers, depending on the circumstances and the goal of expression profiling. See, e.g., manufacturer&#39;s instructions accompanying the product GENEAMP RNA PCR kit (Perkin Elmer, Calif., USA). The derived cDNA can then be used as a template in the subsequent RT-PCR reaction. 
     The PCR step generally uses a thermostable DNA-dependent DNA polymerase, such as the Taq DNA polymerase, which has a 5′-3′ nuclease activity but lacks a 3′-5′ proofreading endonuclease activity. Thus, TAQMAN® PCR typically utilizes the 5′-nuclease activity of Taq or Tth polymerase to hydrolyze a hybridization probe bound to its target amplicon, but any enzyme with equivalent 5′ nuclease activity can be used. Two oligonucleotide primers are used to generate an amplicon typical of a PCR reaction. In one embodiment, the target sequence is shown in Table III. A third oligonucleotide, or probe, is designed to detect nucleotide sequence located between the two PCR primers. The probe is non-extendible by Taq DNA polymerase enzyme, and is labeled with a reporter fluorescent dye and a quencher fluorescent dye. Any laser-induced emission from the reporter dye is quenched by the quenching dye when the two dyes are located close together as they are on the probe. During the amplification reaction, the Taq DNA polymerase enzyme cleaves the probe in a template-dependent manner. The resultant probe fragments disassociate in solution, and signal from the released reporter dye is free from the quenching effect of the second fluorophore. One molecule of reporter dye is liberated for each new molecule synthesized, and detection of the unquenched reporter dye provides the basis for quantitative interpretation of the data. 
     TaqMan® RT-PCR can be performed using commercially available equipment. In a preferred embodiment, the 5′ nuclease procedure is run on a real-time quantitative PCR device such as the ABI PRISM 7900® Sequence Detection System®. The system amplifies samples in a 96-well format on a thermocycler. During amplification, laser-induced fluorescent signal is collected in real-time through fiber optic cables for all 96 wells, and detected at the CCD. The system includes software for running the instrument and for analyzing the data. 5′-Nuclease assay data are initially expressed as Ct, or the threshold cycle. As discussed above, fluorescence values are recorded during every cycle and represent the amount of product amplified to that point in the amplification reaction. The point when the fluorescent signal is first recorded as statistically significant is the threshold cycle (C t ). 
     To minimize errors and the effect of sample-to-sample variation, RT-PCR is usually performed using an internal standard. The ideal internal standard is expressed at a constant level among different tissues, and is unaffected by the experimental treatment. RNAs most frequently used to normalize patterns of gene expression are mRNAs for the housekeeping genes glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) and β-actin. 
     Real time PCR is comparable both with quantitative competitive PCR, where internal competitor for each target sequence is used for normalization, and with quantitative comparative PCR using a normalization gene contained within the sample, or a housekeeping gene for RT-PCR. 
     In another PCR method, i.e., the MassARRAY-based gene expression profiling method (Sequenom, Inc., San Diego, Calif.), following the isolation of RNA and reverse transcription, the obtained cDNA is spiked with a synthetic DNA molecule (competitor), which matches the targeted cDNA region in all positions, except a single base, and serves as an internal standard. The cDNA/competitor mixture is PCR amplified and is subjected to a post-PCR shrimp alkaline phosphatase (SAP) enzyme treatment, which results in the dephosphorylation of the remaining nucleotides. After inactivation of the alkaline phosphatase, the PCR products from the competitor and cDNA are subjected to primer extension, which generates distinct mass signals for the competitor- and cDNA-derived PCR products. After purification, these products are dispensed on a chip array, which is pre-loaded with components needed for analysis with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. The cDNA present in the reaction is then quantified by analyzing the ratios of the peak areas in the mass spectrum generated. 
     Still other embodiments of PCR-based techniques which are known to the art and may be used for gene expression profiling include, e.g., differential display, amplified fragment length polymorphism (iAFLP), and BeadArray™ technology (Illumina, San Diego, Calif.) using the commercially available Luminex100 LabMAP system and multiple color-coded microspheres (Luminex Corp., Austin, Tex.) in a rapid assay for gene expression; and high coverage expression profiling (HiCEP) analysis. 
     D. Microarrays 
     Differential gene expression can also be identified, or confirmed using the microarray technique. Thus, the expression profile of lung cancer-associated genes can be measured in either fresh or paraffin-embedded tissue, using microarray technology. In this method, polynucleotide sequences of interest (including cDNAs and oligonucleotides) are plated, or arrayed, on a microchip substrate. The arrayed sequences are then hybridized with specific DNA probes from cells or tissues of interest. Just as in the other methods and compositions herein, the source of mRNA is total RNA isolated from whole blood of controls and patient subjects. 
     In one embodiment of the microarray technique, PCR amplified inserts of cDNA clones are applied to a substrate in a dense array. In one embodiment, all 559 nucleotide sequences from Table III are applied to the substrate. The microarrayed genes, immobilized on the microchip, are suitable for hybridization under stringent conditions. Fluorescently labeled cDNA probes may be generated through incorporation of fluorescent nucleotides by reverse transcription of RNA extracted from tissues of interest. Labeled cDNA probes applied to the chip hybridize with specificity to each spot of DNA on the array. After stringent washing to remove non-specifically bound probes, the chip is scanned by confocal laser microscopy or by another detection method, such as a CCD camera. Quantitation of hybridization of each arrayed element allows for assessment of corresponding mRNA abundance. With dual color fluorescence, separately labeled cDNA probes generated from two sources of RNA are hybridized pairwise to the array. The relative abundance of the transcripts from the two sources corresponding to each specified gene is thus determined simultaneously. The miniaturized scale of the hybridization affords a convenient and rapid evaluation of the expression pattern for large numbers of genes. Such methods have been shown to have the sensitivity required to detect rare transcripts, which are expressed at a few copies per cell, and to reproducibly detect at least approximately two-fold differences in the expression levels. Microarray analysis can be performed by commercially available equipment, following manufacturer&#39;s protocols. 
     Other useful methods summarized by U.S. Pat. No. 7,081,340, and incorporated by reference herein include Serial Analysis of Gene Expression (SAGE) and Massively Parallel Signature Sequencing (MPSS). Briefly, serial analysis of gene expression (SAGE) is a method that allows the simultaneous and quantitative analysis of a large number of gene transcripts, without the need of providing an individual hybridization probe for each transcript. First, a short sequence tag (about 10 to 14 bp) is generated that contains sufficient information to uniquely identify a transcript, provided that the tag is obtained from a unique position within each transcript. Then, many transcripts are linked together to form long serial molecules, that can be sequenced, revealing the identity of the multiple tags simultaneously. The expression pattern of any population of transcripts can be quantitatively evaluated by determining the abundance of individual tags, and identifying the gene corresponding to each tag. For more details see, e.g. Velculescu et al., Science 270:484 487 (1995); and Velculescu et al., Cell 88:243 51 (1997), both of which are incorporated herein by reference. 
     Gene Expression Analysis by Massively Parallel Signature Sequencing (MPSS), described by Brenner et al., Nature Biotechnology 18:630 634 (2000) (which is incorporated herein by reference), is a sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 μm diameter microbeads. First, a microbead library of DNA templates is constructed by in vitro cloning. This is followed by the assembly of a planar array of the template-containing microbeads in a flow cell at a high density (typically greater than 3×10 6  microbeads/cm 2 ). The free ends of the cloned templates on each microbead are analyzed simultaneously, using a fluorescence-based signature sequencing method that does not require DNA fragment separation. This method has been shown to simultaneously and accurately provide, in a single operation, hundreds of thousands of gene signature sequences from a yeast cDNA library. 
     E. Immunohistochemistry 
     Immunohistochemistry methods are also suitable for detecting the expression levels of the gene expression products of the informative genes described for use in the methods and compositions herein. Antibodies or antisera, preferably polyclonal antisera, and most preferably monoclonal antibodies, or other protein-binding ligands specific for each marker are used to detect expression. The antibodies can be detected by direct labeling of the antibodies themselves, for example, with radioactive labels, fluorescent labels, hapten labels such as, biotin, or an enzyme such as horse radish peroxidase or alkaline phosphatase. Alternatively, unlabeled primary antibody is used in conjunction with a labeled secondary antibody, comprising antisera, polyclonal antisera or a monoclonal antibody specific for the primary antibody. Protocols and kits for immunohistochemical analyses are well known in the art and are commercially available. 
     III. COMPOSITIONS OF THE INVENTION 
     The methods for diagnosing lung cancer described herein which utilize defined gene expression profiles permit the development of simplified diagnostic tools for diagnosing lung cancer, e.g., NSCLC vs. non-cancerous nodule. Thus, a composition for diagnosing lung cancer in a mammalian subject as described herein can be a kit or a reagent. For example, one embodiment of a composition includes a substrate upon which said polynucleotides or oligonucleotides or ligands or ligands are immobilized. In another embodiment, the composition is a kit containing the relevant 5 or more polynucleotides or oligonucleotides or ligands, optional detectable labels for same, immobilization substrates, optional substrates for enzymatic labels, as well as other laboratory items. In still another embodiment, at least one polynucleotide or oligonucleotide or ligand is associated with a detectable label. 
     In one embodiment, a composition for diagnosing lung cancer in a mammalian subject includes 5 or more PCR primer-probe sets. Each primer-probe set amplifies a different polynucleotide sequence from a gene expression product of 5 or more informative genes found in the blood of the subject. These informative genes are selected to form a gene expression profile or signature which is distinguishable between a subject having lung cancer and a subject having a non-cancerous nodule. Changes in expression in the genes in the gene expression profile from that of a reference gene expression profile are correlated with a lung cancer, such as non-small cell lung cancer (NSCLC). 
     In one embodiment of this composition, the informative genes are selected from among the genes identified in Table I. In another embodiment of this composition, the informative genes are selected from among the genes identified in Table II. This collection of genes is those for which the gene product expression is altered (i.e., increased or decreased) versus the same gene product expression in the blood of a reference control (i.e., a patient having a non-cancerous nodule). In one embodiment, polynucleotide or oligonucleotide or ligands, i.e., probes, are generated to 5 or more informative genes from Table I or Table II for use in the composition (the CodeSet). An example of such a composition contains probes to a targeted portion of the 559 genes of Table I. In another embodiment, probes are generated to all 559 genes from Table I for use in the composition. In another embodiment, probes are generated to the first 539 genes from Table I for use in the composition. In another embodiment, probes are generated to the first 3 genes from Table I or Table II for use in the composition. In another embodiment, probes are generated to the first 5 genes from Table I or Table II for use in the composition. In another embodiment, probes are generated to the first 10 genes from Table I or Table II for use in the composition. In another embodiment, probes are generated to the first 15 genes from Table I or Table II for use in the composition. In another embodiment, probes are generated to the first 20 genes from Table I or Table II for use in the composition. In another embodiment, probes are generated to the first 25 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 30 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 35 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 40 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 45 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 50 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 60 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 65 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 70 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 75 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 80 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 85 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 90 genes from Table I or Table II for use in the composition. In yet another embodiment, probes are generated to the first 95 genes from Table I or Table II for use in the composition. In another embodiment, probes are generated to the first 100 genes from Table I or Table II for use in the composition. In another embodiment, probes are generated to the first 200 genes from Table I for use in the composition. In yet another embodiment, probes are generated to 300 genes from Table I for use in the composition. Still other embodiments employ probes to a targeted portion of other combinations of the genes in Table I or Table II. The selected genes from the Table need not be in rank order; rather any combination that clearly shows a difference in expression between the reference control to the diseased patient is useful in such a composition. 
     In one embodiment of the compositions described above, the reference control is a non-healthy control (NHC) as described above. In other embodiments, the reference control may be any class of controls as described above in “Definitions”. 
     The compositions based on the genes selected from Table I or Table II described herein, optionally associated with detectable labels, can be presented in the format of a microfluidics card, a chip or chamber, or a kit adapted for use with the Nanostring, PCR, RT-PCR or Q PCR techniques described above. In one aspect, such a format is a diagnostic assay using TAQMAN® Quantitative PCR low density arrays. In another aspect, such a format is a diagnostic assay using the Nanostring nCounter platform. 
     For use in the above-noted compositions the PCR primers and probes are preferably designed based upon intron sequences present in the gene(s) to be amplified selected from the gene expression profile. Exemplary target sequences are shown in Table III. The design of the primer and probe sequences is within the skill of the art once the particular gene target is selected. The particular methods selected for the primer and probe design and the particular primer and probe sequences are not limiting features of these compositions. A ready explanation of primer and probe design techniques available to those of skill in the art is summarized in U.S. Pat. No. 7,081,340, with reference to publically available tools such as DNA BLAST software, the Repeat Masker program (Baylor College of Medicine), Primer Express (Applied Biosystems); MGB assay-by-design (Applied Biosystems); Primer3 (Steve Rozen and Helen J. Skaletsky (2000) Primer3 on the WWW for general users and for biologist programmers. 
     In general, optimal PCR primers and probes used in the compositions described herein are generally 17-30 bases in length, and contain about 20-80%, such as, for example, about 50-60% G+C bases. Melting temperatures of between 50 and 80° C., e.g. about 50 to 70° C. are typically preferred. 
     In another aspect, a composition for diagnosing lung cancer in a mammalian subject contains a plurality of polynucleotides immobilized on a substrate, wherein the plurality of genomic probes hybridize to 100 or more gene expression products of 100 or more informative genes selected from a gene expression profile in the blood of the subject, the gene expression profile comprising genes selected from Table I. In another embodiment, a composition for diagnosing lung cancer in a mammalian subject contains a plurality of polynucleotides immobilized on a substrate, wherein the plurality of genomic probes hybridize to 10 or more gene expression products of 10 or more informative genes selected from a gene expression profile in the blood of the subject, the gene expression profile comprising genes selected from Table I or Table II. This type of composition relies on recognition of the same gene profiles as described above for the Nanostring compositions but employs the techniques of a cDNA array. Hybridization of the immobilized polynucleotides in the composition to the gene expression products present in the blood of the patient subject is employed to quantitate the expression of the informative genes selected from among the genes identified in Tables I or Table II to generate a gene expression profile for the patient, which is then compared to that of a reference sample. As described above, depending upon the identification of the profile (i.e., that of genes of Table I or subsets thereof, that of genes of Table II or subsets thereof), this composition enables the diagnosis and prognosis of NSCLC lung cancers. Again, the selection of the polynucleotide sequences, their length and labels used in the composition are routine determinations made by one of skill in the art in view of the teachings of which genes can form the gene expression profiles suitable for the diagnosis and prognosis of lung cancers. 
     In yet another aspect, a composition or kit useful in the methods described herein contain a plurality of ligands that bind to 100 or more gene expression products of 100 or more informative genes selected from a gene expression profile in the blood of the subject. In another embodiment, a composition or kit useful in the methods described herein contain a plurality of ligands that bind to 10 or more gene expression products of 10 or more informative genes selected from a gene expression profile in the blood of the subject. The gene expression profile contains the genes of Table I or Table II, as described above for the other compositions. This composition enables detection of the proteins expressed by the genes in the indicated Tables. While preferably the ligands are antibodies to the proteins encoded by the genes in the profile, it would be evident to one of skill in the art that various forms of antibody, e.g., polyclonal, monoclonal, recombinant, chimeric, as well as fragments and components (e.g., CDRs, single chain variable regions, etc.) may be used in place of antibodies. Such ligands may be immobilized on suitable substrates for contact with the subject&#39;s blood and analyzed in a conventional fashion. In certain embodiments, the ligands are associated with detectable labels. These compositions also enable detection of changes in proteins encoded by the genes in the gene expression profile from those of a reference gene expression profile. Such changes correlate with lung cancer in a manner similar to that for the PCR and polynucleotide-containing compositions described above. 
     For all of the above forms of diagnostic/prognostic compositions, the gene expression profile can, in one embodiment, include at least the first 25 of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 10 or more of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 15 or more of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 20 or more of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 30 or more of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 40 or more of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 50 or more of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 60 or more of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 70 or more of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 80 or more of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 90 or more of the informative genes of Table I or Table II. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include all 100 of the informative genes of Table II. In one embodiment, for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include at least the first 100 of the informative genes of Table I. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 200 or more of the informative genes of Table I. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 300 or more of the informative genes of Table I. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 400 or more of the informative genes of Table I. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 500 or more of the informative genes of Table I. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include 539 or more of the informative genes of Table I. In another embodiment for all of the above forms of diagnostic/prognostic compositions, the gene expression profile can include all 559 of the informative genes of Table I. 
     These compositions may be used to diagnose lung cancers, such as stage I or stage II NSCLC. Further these compositions are useful to provide a supplemental or original diagnosis in a subject having lung nodules of unknown etiology. 
     IV. DIAGNOSTIC METHODS OF THE INVENTION 
     All of the above-described compositions provide a variety of diagnostic tools which permit a blood-based, non-invasive assessment of disease status in a subject. Use of these compositions in diagnostic tests, which may be coupled with other screening tests, such as a chest X-ray or CT scan, increase diagnostic accuracy and/or direct additional testing. 
     Thus, in one aspect, a method is provided for diagnosing lung cancer in a mammalian subject. This method involves identifying a gene expression profile in the blood of a mammalian, preferably human, subject. In one embodiment, the gene expression profile includes 100 or more gene expression products of 100 or more informative genes having increased or decreased expression in lung cancer. The gene expression profiles are formed by selection of 100 or more informative genes from the genes of Table I. In another embodiment, the gene expression profile includes 10 or more gene expression products of 10 or more informative genes having increased or decreased expression in lung cancer. The gene expression profiles are formed by selection of 10 or more informative genes from the genes of Table I. In another embodiment, the gene expression profiles are formed by selection of 10 or more informative genes from the genes of Table II. In another embodiment, the gene expression profile includes 10 or more gene expression products of 5 or more informative genes having increased or decreased expression in lung cancer. The gene expression profiles are formed by selection of 5 or more informative genes from the genes of Table I. In another embodiment, the gene expression profiles are formed by selection of 5 or more informative genes from the genes of Table II. Comparison of a subject&#39;s gene expression profile with a reference gene expression profile permits identification of changes in expression of the informative genes that correlate with a lung cancer (e.g., NSCLC). This method may be performed using any of the compositions described above. In one embodiment, the method enables the diagnosis of a cancerous tumor from a benign nodule. 
     In another aspect, use of any of the compositions described herein is provided for diagnosing lung cancer in a subject. 
     The diagnostic compositions and methods described herein provide a variety of advantages over current diagnostic methods. Among such advantages are the following. As exemplified herein, subjects with cancerous tumors are distinguished from those with benign nodules. These methods and compositions provide a solution to the practical diagnostic problem of whether a patient who presents at a lung clinic with a small nodule has malignant disease. Patients with an intermediate-risk nodule would clearly benefit from a non-invasive test that would move the patient into either a very low-likelihood or a very high-likelihood category of disease risk. An accurate estimate of malignancy based on a genomic profile (i.e. estimating a given patient has a 90% probability of having cancer versus estimating the patient has only a 5% chance of having cancer) would result in fewer surgeries for benign disease, more early stage tumors removed at a curable stage, fewer follow-up CT scans, and reduction of the significant psychological costs of worrying about a nodule. The economic impact would also likely be significant, such as reducing the current estimated cost of additional health care associated with CT screening for lung cancer, i.e., $116,000 per quality adjusted life-year gained. A non-invasive blood genomics test that has a sufficient sensitivity and specificity would significantly alter the post-test probability of malignancy and thus, the subsequent clinical care. 
     A desirable advantage of these methods over existing methods is that they are able to characterize the disease state from a minimally-invasive procedure, i.e., by taking a blood sample. In contrast, current practice for classification of cancer tumors from gene expression profiles depends on a tissue sample, usually a sample from a tumor. In the case of very small tumors a biopsy is problematic and clearly if no tumor is known or visible, a sample from it is impossible. No purification of tumor is required, as is the case when tumor samples are analyzed. A recently published method depends on brushing epithelial cells from the lung during bronchoscopy, a method which is also considerably more invasive than taking a blood sample. Blood samples have an additional advantage, which is that the material is easily prepared and stabilized for later analysis, which is important when messenger RNA is to be analyzed. 
     The 559 classifier described herein showed a ROC-AUC of 0.81 over all tested samples. In one embodiment, when the sensitivity is about 90%, the specificity is about 46%. When the nodule classification accuracy is assessed by size without using a specific threshold for sensitivity, as nodules size and the cancer risk factor increases, the number of benign nodules classified as cancer increases. In one embodiment, the accuracy of the gene classifier is about 89% for nodules ≤8 mm. In another embodiment, the accuracy of the gene classifier is about 75% for nodules &gt;8 to about ≤12 mm. In yet another embodiment, the accuracy of the gene classifier is about 68% for nodules &gt;12 to about ≤16 mm. In another embodiment, the accuracy of the gene classifier is about 53% for &gt;16 mm. See examples below. 
     In one embodiment, for nodules about &lt;10 mm, the specificity is about 54% and the ROC-AUC to 0.85 at about 90% sensitivity. In another embodiment, for larger nodules, about &gt;10 mm, the specificity is about 24% and the ROC-AUC about 0.71 at about 90% sensitivity. 
     The 100 Classifier described herein showed a ROC-AUC of 0.82 over all tested samples. In one embodiment, when the sensitivity is about 90%, the specificity is about 62%. In another embodiment, when the sensitivity is about 79%, the specificity is about 68%. In one embodiment, when the sensitivity is about 71%, the specificity is about 75%. See examples below. 
     These compositions and methods allow for more accurate diagnosis and treatment of lung cancer. Thus, in one embodiment, the methods described include treatment of the lung cancer. Treatment may removal of the neoplastic growth, chemotherapy and/or any other treatment known in the art or described herein. 
     In one embodiment, a method for diagnosing the existence or evaluating a lung cancer in a mammalian subject is provided, which includes identifying changes in the expression of 5, 10, 15 or more genes in the sample of said subject, said genes selected from the genes of Table I or the genes of Table II. The subject&#39;s gene expression levels are compare with the levels of the same genes in a reference or control, wherein changes in expression of the subject&#39;s genes from those of the reference correlates with a diagnosis or evaluation of a lung cancer. 
     In one embodiment, the diagnosis or evaluation comprise one or more of a diagnosis of a lung cancer, a diagnosis of a benign nodule, a diagnosis of a stage of lung cancer, a diagnosis of a type or classification of a lung cancer, a diagnosis or detection of a recurrence of a lung cancer, a diagnosis or detection of a regression of a lung cancer, a prognosis of a lung cancer, or an evaluation of the response of a lung cancer to a surgical or non-surgical therapy. In another embodiment, the changes comprise an upregulation of one or more selected genes in comparison to said reference or control or a downregulation of one or more selected genes in comparison to said reference or control. 
     In one embodiment, the method includes the size of a lung nodule in the subject. The specificity and sensitivity may be variable based on the size of the nodule. In one embodiment, the specificity is about 46% at about 90% sensitivity. In another embodiment, the specificity is about 54% at about 90% sensitivity for nodules &lt;10 mm. In yet another embodiment, the accuracy is about 88% for nodules ≤8 mm, about 75% for nodules &gt;8 mm and ≤12 mm, about 68% for nodules &gt;12 mm and ≤16 mm, and about 53% for nodules &gt;16 mm. 
     In another embodiment, the reference or control comprises three or more genes of Table I sample of at least one reference subject. The reference subject may be selected from the group consisting of: (a) a smoker with malignant disease, (b) a smoker with non-malignant disease, (c) a former smoker with non-malignant disease, (d) a healthy non-smoker with no disease, (e) a non-smoker who has chronic obstructive pulmonary disease (COPD), (f) a former smoker with COPD, (g) a subject with a solid lung tumor prior to surgery for removal of same; (h) a subject with a solid lung tumor following surgical removal of said tumor; (i) a subject with a solid lung tumor prior to therapy for same; and (j) a subject with a solid lung tumor during or following therapy for same. In one embodiment, the reference or control subject (a)-(j) is the same test subject at a temporally earlier timepoint. 
     The sample is selected from those described herein. In one embodiment, the sample is peripheral blood. The nucleic acids in the sample are, in some embodiments, stabilized prior to identifying changes in the gene expression levels. Such stabilization may be accomplished, e.g., using the Pax Gene system, described herein. 
     In one embodiment, the method of detecting lung cancer in a patient includes 
     a. obtaining a sample from the patient; and 
     b. detecting a change in expression in at least 10 genes selected from Table I or Table II in the patient sample as compared to a control by contacting the sample with a composition comprising oligonucleotides, polynucleotides or ligands specific for each different gene transcript or expression product of the at least 10 gene of Table I or Table II and detecting binding between the oligonucleotide, polynucleotide or ligand and the gene product or expression product. 
     In another embodiment, the method of diagnosing lung cancer in a subject includes 
     a. obtaining a blood sample from a subject; 
     b. detecting a change in expression in at least 10 genes selected from Table I or Table II in the patient sample as compared to a control by contacting the sample with a composition comprising oligonucleotides, polynucleotides or ligands specific for each different gene transcript or expression product of the at least 100 gene of Table I or Table II and detecting binding between the oligonucleotide, polynucleotide or ligand and the gene product or expression product; and 
     c. diagnosing the subject with cancer when changes in expression of the subject&#39;s genes from those of the reference are detected. 
     In yet another embodiment, the method includes 
     a. obtaining a blood sample from a subject; 
     b. detecting a change in expression in at least 10 genes selected from Table I or Table II in the patient sample as compared to a control by contacting the sample with a composition comprising oligonucleotides, polynucleotides or ligands specific for each different gene transcript or expression product of the at least 10 genes of Table I or Table II and detecting binding between the oligonucleotide, polynucleotide or ligand and the gene product or expression product; 
     c. diagnosing the subject with cancer when changes in expression of the subject&#39;s genes from those of the reference are detected; and 
     d. removing the neoplastic growth. 
     V. EXAMPLES 
     The invention is now described with reference to the following examples. These examples are provided for the purpose of illustration only and the invention should in no way be construed as being limited to these examples but rather should be construed to encompass any and all variations that become evident as a result of the teaching provided herein. 
     Example 1: Patient Population—Analysis A 
     For development of the gene classifier described herein, blood samples and clinical information were collected from 150 subjects, 73 having a diagnosis of lung cancer and 77 having a diagnosis of benign nodule. Patient characteristics are shown in  FIG.  1   . 
     Patients with lung cancer included newly diagnosed male and female patients with early stage lung cancer. They were in moderately good health (ambulatory), although with medical illness. They were excluded if they have had previous cancers, chemotherapy, radiation, or cancer surgery. They must have had a lung cancer diagnosis within preceding 6 months, histologic confirmation, and no systemic therapy, such as chemotherapy, radiation therapy or cancer surgery as biomarker levels may change with therapy. Thus the majority of the cancer patients were early stage (i.e., Stage I and Stage II). 
     The “control” cohort was derived from patients with benign lung nodules (e.g. ground glass opacities, single nodules, granulomas or hamartomas). These patients were evaluated at pulmonary clinics, or underwent thoracic surgery for a lung nodule. All samples were collected prior to surgery. 
     Example 2: Patient Population—Analysis B 
     Further blood samples and clinical information were collected from 120 subjects, 60 having a diagnosis of lung cancer and 60 having a diagnosis of benign nodule. Patients with lung cancer included newly diagnosed male and female patients with early stage lung cancer. They were in moderately good health (ambulatory), although with medical illness. They were excluded if they have had previous cancers, chemotherapy, radiation, or cancer surgery. They must have had a lung cancer diagnosis within preceding 6 months, histologic confirmation, and no systemic therapy, such as chemotherapy, radiation therapy or cancer surgery as biomarker levels may change with therapy. Thus the majority of the cancer patients were early stage (i.e., Stage I and Stage II). 
     The “control” cohort was derived from patients with benign lung nodules (e.g. granulomas or hamartomas). These patients were evaluated at pulmonary clinics, or underwent thoracic surgery for a lung nodule. All samples were collected prior to surgery. 
     Example 3: Sample Collection Protocols and Processing 
     Blood samples were collected in the clinic by the tissue acquisition technician. Blood samples were drawn directly into PAXgene Blood RNA Tubes via standard phlebotomy technique. These tubes contain a proprietary reagent that immediately stabilizes intracellular RNA, minimizing the ex-vivo degradation or up-regulation of RNA transcripts. The ability to eliminate freezing, batch samples, and to minimize the urgency to process samples following collection, greatly enhances lab efficiency and reduces costs. 
     Example 4—RNA Purification and Quality Assessment 
     PAXgene RNA is prepared using a standard commercially available kit from Qiagen™ that allows purification of mRNA. The resulting RNA is used for mRNA profiling. The RNA quality is determined using a Bioanalyzer. Only samples with RNA Integrity numbers &gt;3 were used. 
     Briefly, RNA is isolated as follows. Turn shaker-incubator on and set to 55° C. before beginning. Unless otherwise noted, all steps in this protocol including centrifugation steps, should be carried out at room temp (15-25° C.). This protocol assumes samples are stores at −80° C. Unfrozen samples that have been left a RT per the Qiagen protocol of a minimum of 2 hours should be processed in the same way. 
     Thaw Paxgene tubes upright in a plastic rack. Invert tubes at least 10 times to mix before starting isolation. Prepare all necessary tubes. For each sample, the following are needed: 2 numbered 1.5 ml Eppendorf tubes; 1 Eppendorf tube with the sample information (this is the final tube); 1 Lilac Paxgene spin column; 1 Red Paxgene Spin column; and 5 Processing tubes. 
     Centrifuge the PAXgene Blood RNA Tube for 10 minutes at 5000×g using a swing-out rotor in Qiagen centrifuge. (Sigma 4-15° C. Centrifuge., Rotor: Sigma Nr. 11140, 7/01, 5500/min, Holder: Sigma 13115, 286 g 14/D, Inside tube holder: 18010, 125 g). Note: After thawed, ensure that the blood sample has been incubated in the PAXgene Blood RNA Tube for a minimum of 2 hours at room temperature (15-25° C.), in order to achieve complete lysis of blood cells. 
     Under the hood—remove the supernatant by decanting into bleach. When the supernatant is decanted, take care not to disturb the pellet, and dry the rim of the tube with a clean paper towel. Discard the decanted supernatant by placing the clotted blood into a bag and then into the infectious waste and discard the fluid portion down the sink and wash down with a lot of water. Add 4 ml RNase-free water to the pellet, and close the tube using a fresh secondary Hemogard closure. 
     Vortex until the pellet is visibly dissolved. Weigh the tubes in the centrifuge holder again to ensure they are balanced, and centrifuge for 10 minutes at 5000×g using a swing-out rotor Qiagen centrifuge Small debris remaining in the supernatant after vortexing but before centrifugation will not affect the procedure. 
     Remove and discard the entire supernatant. Leave tube upside-down for 1 min to drain off all supernatant. Incomplete removal of the supernatant will inhibit lysis and dilute the lysate, and therefore affect the conditions for binding RNA to the PAXgene membrane. 
     Add 350 μl Buffer BM1 and pipet up and down lyse the pellet. 
     Pipet the re-suspended sample into a labeled 1.5 ml microcentrifuge tube. Add 300 μl Buffer BM2. Then add 40 μl proteinase K. Mix by vortexing for 5 seconds, and incubate for 10 minutes at 55° C. using a shaker-incubator at the highest possible speed, 800 rpm on Eppendorf thermomixer. (If using a shaking water bath instead of a thermomixer, quickly vortex the samples every 2-3 minutes during the incubation. Keep the vortexer next to the incubator). 
     Pipet the lysate directly into a PAXgene Shredder spin column (lilac tube) placed in a 2 ml processing tube, and centrifuge for 3 minutes at 24 C at 18,500×g in the TOMY Microtwin centrifuge. Carefully pipet the lysate into the spin column and visually check that the lysate is completely transferred to the spin column. To prevent damage to columns and tubes, do not exceed 20,000×g. 
     Carefully transfer the entire supernatant of the flow-through fraction to a fresh 1.5 ml microcentrifuge tube without disturbing the pellet in the processing tube. Discard the pellet in the processing tube. 
     Add 700 μl isopropanol (100%) to the supernatant. Mix by vortexing. 
     Pipet 690 μl sample into the PAXgene RNA spin column (red) placed in a 2 ml processing tube, and centrifuge for 1 minute at 10,000×g. Place the spin column in a new 2 ml processing tube, and discard the old processing tube containing flow-through. 
     Pipet the remaining sample into the PAXgene RNA spin column (red), and centrifuge for 1 minute at 18,500×g. Place the spin column in a new 2 ml processing tube, and discard the old processing tube containing flow-through. Carefully pipet the sample into the spin column and visually check that the sample is completely transferred to the spin column. 
     Pipet 350 μl Buffer BM3 into the PAXgene RNA spin column. Centrifuge for 15 sec at 10,000×g. Place the spin column in a new 2 ml processing tube, and discard the old processing tube containing flow-through. 
     Prepare DNase I incubation mix for step 13. Add 10 μl DNase I stock solution to 70 μl Buffer RDD in a 1.5 ml microcentrifuge tube. Mix by gently flicking the tube, and centrifuge briefly to collect residual liquid from the sides of the tube. 
     Pipet the DNase I incubation mix (80 μl) directly onto the PAXgene RNA spin column membrane, and place on the benchtop (20-30° C.) for 15 minutes. Ensure that the DNase I incubation mix is placed directly onto the membrane. DNase digestion will be incomplete if part of the mix is applied to and remains on the walls or the O-ring of the spin column. 
     Pipet 350 μl Buffer BM3 into the PAXgene RNA spin column, and centrifuge for 15 sec at 18,500×g. Place the spin column in a new 2 ml processing tube, and discard the old processing tube containing flow-through. 
     Pipet 500 μl Buffer BM4 to the PAXgene RNA spin column, and centrifuge for 15 sec at 10,000×g. Place the spin column in a new 2 ml processing tube, and discard the old processing tube containing flow-through. 
     Add another 500 μl Buffer BM4 to the PAXgene RNA spin column. Centrifuge for 2 minutes at 18,500×g. 
     Discard the tube containing the flow-through, and place the PAXgene RNA spin column in a new 2 ml processing tube. Centrifuge for 1 minute at 18,500×g. 
     Discard the tube containing the flow-through. Place the PAXgene RNA spin column in a labeled 1.5 ml microcentrifuge tube (final tube), and pipet 40 μl Buffer BR5 directly onto the PAXgene RNA spin column membrane. Centrifuge for 1 minute at 10,000×g to elute the RNA. It is important to wet the entire membrane with Buffer BR5 in order to achieve maximum elution efficiency. 
     Repeat the elution step as described, using 40 μl Buffer BR5 and the same microcentrifuge tube. Centrifuge for 1 minute at 20,000×g to elute the RNA. 
     Incubate the eluate for 5 minutes at 65° C. in the shaker-incubator without shaking. After incubation, chill immediately on ice. This incubation at 65° C. denatures the RNA for downstream applications. Do not exceed the incubation time or temperature. 
     If the RNA samples will not be used immediately, store at −20° C. or −70° C. Since the RNA remains denatured after repeated freezing and thawing, it is not necessary to repeat the incubation at 65° C. 
     Example 5: Measurement of RNA Levels 
     To provide a biomarker signature that can be used in clinical practice to diagnose lung cancer, a gene expression profile with the smallest number of genes that maintain satisfactory accuracy is provided by the use of 100 more of the genes identified in Table I as well as by the use of 10 or more of the genes identified in Table II. These gene profiles or signatures permit simpler and more practical tests that are easy to use in a standard clinical laboratory. Because the number of discriminating genes is small enough, NanoString nCounter® platforms are developed using these gene expression profiles. 
     A. Nanostring nCounter® Platform Gene Expression Assay Protocol 
     Total RNA was isolated from whole blood using the Paxgene Blood miRNA Kit, as described above, and samples were checked for RNA quality. Samples were analyzed with the Agilent 2100 Bioanalyzer on a RNA Nano chip, using the RIN score and electropherogram picture as indicators for good sample integrity. Samples were also quantitated on the Nanodrop (ND-1000 Spectrophotometer) where 260/280 and 260/230 readings were recorded and evaluated for Nanostring-compatibility. From the concentrations taken by Nanodrop, total RNA samples were normalized to contain 100 ng in 5 μL, using Nuclease-free water as diluent, into Nanostring-provided tube strips. An 8 μL aliquot of a mixture of the Nanostring nCounter Reporter CodeSet and Hybridization Buffer (70 μL Hybridization Buffer, 42 μL Reporter CodeSet per 12 assays) and 2 μL of Capture ProbeSet was added to each 5 μL RNA sample. Samples were hybridized for 19 hours at 65° C. in the Thermocycler (Eppendorf). During hybridization, Reporter Probes, which have fluorescent barcodes specific to each mRNA of interest to the user, and biotinylated Capture Probes bound to their associated target mRNA to create target-probe complexes. After hybridization was complete, samples were then transferred to the nCounter Prep Station for processing using the Standard Protocol setting (Run Time: 2 hr35 min). The Prep Station robot, during the Standard Protocol, washed samples to remove excess Reporter and Capture Probes. Samples were moved to a streptavidin-coated cartridge where purified target-probe complexes were immobilized in preparation for imaging by the nCounter Digital Analyzer. Upon completion, the cartridge was sealed and placed in the Digital Analyzer using a Field of View (FOV) setting at 555. A fluorescent microscope tabulated the raw counts for each unique barcode associated with a target mRNA. Data collected was stored in .csv files and then transferred to the Bioinformatics Facility for analysis according to the manufacturer&#39;s instructions. 
     Example 6: Biomarker Selection 
     Support Vector Machine (SVM) can be applied to gene expression datasets for gene function discovery and classification. SVM has been found to be most efficient at distinguishing the more closely related cases and controls that reside in the margins. Primarily SVM-RFE (48, 54) was used to develop gene expression classifiers which distinguish clinically defined classes of patients from clinically defined classes of controls (smokers, non-smokers, COPD, granuloma, etc). SVM-RFE is a SVM based model utilized in the art that removes genes, recursively based on their contribution to the discrimination, between the two classes being analyzed. The lowest scoring genes by coefficient weights were removed and the remaining genes were scored again and the procedure was repeated until only a few genes remained. This method has been used in several studies to perform classification and gene selection tasks. However, choosing appropriate values of the algorithm parameters (penalty parameter, kernel-function, etc.) can often influence performance. 
     SVM-RCE is a related SVM based model, in that it, like SVM-RFE assesses the relative contributions of the genes to the classifier. SVM-RCE assesses the contributions of groups of correlated genes instead of individual genes. Additionally, although both methods remove the least important genes at each step, SVM-RCE scores and removes clusters of genes, while SVM-RFE scores and removes a single or small numbers of genes at each round of the algorithm. 
     The SVM-RCE method is briefly described here. Low expressing genes (average expression less than 2× background) were removed, quantile normalization performed, and then “outlier” arrays whose median expression values differ by more than 3 sigma from the median of the dataset were removed. The remaining samples were subject to SVM-RCE using ten repetitions of 10-fold cross-validation of the algorithm. The genes were reduced by t-test (applied on the training set) to an experimentally determined optimal value which produces highest accuracy in the final result. These starting genes were clustered by K-means into clusters of correlated genes whose average size is 3-5 genes. SVM classification scoring was carried out on each cluster using 3-fold resampling repeated 5 times, and the worst scoring clusters eliminated. Accuracy is determined on the surviving pool of genes using the left-out 10% of samples (testing set) and the top-scoring 100 genes were recorded. The procedure was repeated from the clustering step to an end point of 2 clusters. The optimal gene panel was taken to be the minimal number of genes which gives the maximal accuracy starting with the most frequently selected gene. The identity of the individual genes in this panel is not fixed, since the order reflects the number of times a given gene was selected in the top 100 informative genes and this order is subject to some variation. 
     A. Biomarker Selection. 
     Genes which score highest (by SVM) in discriminating cancerous tumors from benign nodules were examined for their utility for clinical tests. Factors considered include, higher differences in expression levels between classes, and low variability within classes. When selecting biomarkers for validation an effort was made to select genes with distinct expression profiles to avoid selection of correlated genes and to identify genes with differential expression levels that were robust by alternative techniques including PCR and/or immuno-histochemistry. 
     B. Validation. 
     Three methods of validation were considered. 
     Cross-Validation: To minimize over-fitting within a dataset, K-fold cross-validation (K usually equal to 10) was used, when the dataset is split on K parts randomly and K−1 parts were used for training and 1 for testing. Thus, for K=10 the algorithm was trained on a random selection of 90% of the patients and 90% of the controls and then tested on the remaining 10%. This was repeated until all of the samples have been employed as test subjects and the cumulated classifier makes use of all of the samples, but no sample is tested using a training set of which it is a part. To reduce the randomization impact, K-fold separation was performed M times producing different combinations of patients and controls in each of K folds each time. Therefore, for individual dataset M*K rounds of permuted selection of training and testing sets were used for each set of genes. 
     Independent Validation: To estimate the reproducibility of the data and the generality of the classifier, one needs to examine the classifier that was built using one dataset and tested using another dataset to estimate the performance of the classifier. To estimate the performance, validation on the second set was performed using the classifier developed with the original dataset. 
     Resampling (permutation): To demonstrate dependence of the classifier on the disease state, patients and controls from the dataset were chosen at random (permuted) and the classification was repeated. The accuracy of classification using randomized samples was compared to the accuracy of the developed classifier to determine the p value for the classifier, i.e., the possibility that the classifier might have been chosen by chance. In order to test the generality of a classifier developed in this manner, it was used to classify independent sets of samples that were not used in developing the classifier. The cross-validation accuracies of the permuted and original classifier were compared on independent test sets to confirm its validity in classifying new samples. 
     C. Classifier Performance 
     Performance of each classifier was estimated by different methods and several performance measurements were used for comparing classifiers between each other. These measurements include accuracy, area under ROC curve, sensitivity, specificity, true positive rate and true negative rate. Based on the required properties of the classification of interest, different performance measurements can be used to pick the optimal classifier, e.g. classifier to use in screening of the whole population would require better specificity to compensate for small (˜1%) prevalence of the disease and therefore avoid large number of false positive hits, while a diagnostic classifier of patients in hospital should be more sensitive. 
     For diagnosing cancerous tumors from benign nodules, higher sensitivity is more desirable than specificity, as the patients are already at high risk. 
     Example 7: Testing of the Classifiers 
     Peripheral blood samples were all collected in PAXgene RNA stabilizations tubes and RNA was extracted according to the manufacturer. Samples were tested on a Nanostring nCounter™ (as described above) against a custom panel of 559 probes (Table III). In addition, they were tested against a 100 probe subset of 559 marker panel. 
     For the 559 Classifier, 432 were selected based on previous microarray data, 107 probes were selected from Nanostring studies and 20 were housekeeping genes. We analyzed 610 PAXgene RNA samples (278 cancers, 332 controls) derived from 5 collection sites. For QC, a Universal RNA standard (Agilent) was included in each batch of 36 samples tested. Probe expression values were normalized using the 20 housekeeping genes as well as spike-in positive and negative controls supplied by Nanostring (included in classifier). Zscores were calculated for probe count values and served as the input to a Support Vector Machine (SVM) classifier using a polynomial kernel. Classification performance was evaluated by 10-fold cross-validation of the samples. 
     A. 559 Classifier 
     As shown in  FIGS.  2 A to  2 B , the 559 classifier developed on all the samples showed a ROC-AUC of 0.81 ( FIG.  2 A ). With the Sensitivity set at 90%, the specificity is 46%. When performed on a balanced set of 556 samples (278 cancer, 278 nodule), similar performance is shown ( FIG.  2 B ). For both sets, UHR controls, post samples, and patients with other cancers were excluded. 
     When nodule classification accuracy is assessed by size without using a specific threshold for sensitivity, we find that as nodules size and the cancer risk factor increases, the number of benign nodules classified as cancer increases.  FIG.  3   . In this analysis, nodules ≤8 mm were correctly classified 88.9% of the time, for nodules &gt;8, ≤12 mm accuracy was 75%, for nodules &gt;12, ≤16 mm accuracy was 68%, for nodules &gt;16 mm accuracy is 53.6%. See Table IV below. 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE IV 
               
               
                   
               
               
                 Nodule Size 
                 Correct 
                 Incorrect 
                 Total 
                 Specificity 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 &lt;=5 mm 
                 108 
                 19 
                 127 
                 85.0% 
               
               
                 &gt;5, &lt;=8 mm 
                 88 
                 11 
                 99 
                 88.9% 
               
               
                  &gt;8, &lt;=12 mm 
                 40 
                 13 
                 53 
                 75.5% 
               
               
                 &gt;12, &lt;=16 mm 
                 17 
                 8 
                 25 
                 68.0% 
               
               
                 &gt;16 mm 
                 15 
                 13 
                 28 
                 53.6% 
               
               
                 Total 
                 268 
                 64 
                 332 
                 80.7% 
               
               
                   
               
            
           
         
       
     
     A second set of nodules was tested and the accuracy of the classifier for size groups was determined by sample group (cancer vs benign nodule). Similarly, as nodule size and the cancer risk factor increases, the number of benign nodules classified as cancer increases ( FIGS.  4 A to  4 C ). For cancers &gt;5 mm and higher, r=0.95. For nodules of all sizes, r=0.97. The chart shows the sensitivity and specificity of the classification of cancers and nodules based on lesion size. These numbers are shown in bar graph form below. 
     Since classification accuracy was found to be negatively correlated with benign nodule size, we reanalyzed the data using only nodules &lt;10 mm (n=244) ( FIG.  5 A ) and sensitivity fixed at 90%, in this case the specificity rises to 54% and the ROC-AUC to 0.85. For larger nodules, &gt;10 mm (n=88) the specificity drops to 24% and the ROC-AUC drops to 0.71 ( FIG.  5 B ). See Table V below. 
     
       
         
           
               
               
               
               
             
               
                 TABLE V 
               
               
                   
               
               
                   
                 Small 
                 Large 
                   
               
               
                   
                 ≤10 mm 
                 &gt;10 mm 
                 All nodules 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 N (nodules) 
                 244 
                 88 
                 332 
               
               
                   
                 min 
                 1 
                 10.4 
                 1 
               
               
                   
                 max 
                 10 
                 90 
                 90 
               
               
                   
                 mean 
                 6.07 
                 17.8 
                 8.7 
               
               
                   
                 median 
                 6 
                 15 
                 6 
               
               
                   
                 std 
                 1.73 
                 10.6 
                 7.13 
               
               
                   
                 ROC Area 
                 0.85 
                 0.71 
                 0.81 
               
               
                   
                 Specificity at 
                 54% 
                 42% 
                 46% 
               
               
                   
                 90% Sensitivity 
               
               
                   
               
            
           
         
       
     
     B. 100 Marker Classifier 
     We now reanalyzed the data from the 633 samples analyzed by W559 on the Nanostring platform in order to identify the minimal number of probes required to maintain performance attained with the whole panel. We used SVM-RFE for probe selection as previously described. We used 75% of the data for the training set with SVM-RFE and the tested the performance of top 100 probes (Table II) selected by this process on an independent testing set composed of 25% of the samples. Samples were randomly selected for training and testing sets Table VI below. The accuracy obtained on the testing set is shown in  FIG.  6   . In this analysis, at a sensitivity of 90%, specificity was 62%; at a sensitivity of 79%, specificity was 68%; and at a sensitivity of 71%, specificity was 75% ( FIG.  6   ). In summary the ROC-AUC is 0.82 and at a sensitivity of 0.90 we achieve a specificity of 0.62. 
     
       
         
           
               
               
               
             
               
                 TABLE VI 
               
             
            
               
                   
               
               
                   
                 nodules 
                 cancer 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 &gt; 
                 &lt;= 
                 n 
                 &gt; 
                 &lt;= 
                 n 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 0 
                 5 
                 130 
                 0 
                 14 
                 86 
               
               
                   
                 5 
                 8 
                 109 
                 14 
                 22 
                 75 
               
               
                   
                 8 
                 12.5 
                 65 
                 22 
                 33 
                 64 
               
               
                   
                 12.5 
                   
                 57 
                 33 
                   
                 47 
               
               
                   
               
            
           
         
       
     
     Each and every patent, patent application, and publication, including the priority application, U.S. Provisional Patent Application No. 62/352,865, filed Jun. 21, 2017, and publically available gene sequence cited throughout the disclosure is expressly incorporated herein by reference in its entirety. While this invention has been disclosed with reference to specific embodiments, it is apparent that other embodiments and variations of this invention are devised by others skilled in the art without departing from the true spirit and scope of the invention. The appended claims include such embodiments and equivalent variations.