Patent Publication Number: US-2017369904-A1

Title: Methods for increasing cell culture transfection efficiency and cellular reprogramming

Description:
CROSS REFERENCE 
     This application claims the benefit of U.S. Provisional Application No. 62/405,725, filed Oct. 7, 2016; U.S. Provisional Application No. 62/362,214, filed Jul. 14, 2016; and U.S. Provisional Application No. 62/353,435, filed Jun. 22, 2016, each of which is incorporated herein by reference in its entirety. 
    
    
     BACKGROUND 
     Transfection methods can be used to introduce nucleic acids into cultured cells. Transfection methods have become a mainstay of studies related to gene regulation, gene function, molecular therapy, signal transduction, drug screening, and gene therapy. Transfection efficiency can vary based on cell culture conditions, cell type, cell viability and health, cell confluency, cell culture media, serum, and type of nucleic acid used for transfection. A method for increasing cell culture transfection efficiency could lead to improvements in genetic manipulation of cells and, in turn, future therapeutic studies. 
     Stem cell reprogramming is a cell culture technique that can be used in the field of regenerative medicine. Induced pluripotent stem cells (iPSCs) can be used to replace those cells lost due to damage or disease in afflicted patients. Current methods of stem cell reprogramming can be inefficient and time-consuming. Thus, a method for increasing stem cell reprogramming efficiency could lead to improvements in future therapeutic studies. 
     SUMMARY OF THE INVENTION 
     In some embodiments, the invention provides a method for increasing transfection efficiency of a nucleic acid that is introduced into a cell, the method comprising culturing the cell in a hypoxic condition and a positive pressure condition, wherein culturing the cell in the hypoxic condition and the positive pressure condition increases expression of a polypeptide encoded by the nucleic acid that is introduced into the cell as compared to expression of the polypeptide encoded by a nucleic acid that is introduced into a cell that is cultured in the absence of the hypoxic condition and the positive pressure condition. 
     In some embodiments, the invention provides a method for reprogramming a cell, the method comprising culturing the cell in a hypoxic condition and a positive pressure condition, wherein the cell exhibits a rate of reprogramming that is higher than the rate of reprogramming of a cell cultured in the absence of the hypoxic condition and the positive pressure condition. 
     INCORPORATION BY REFERENCE 
     Each patent, publication, and non-patent literature cited in the application is hereby incorporated by reference in its entirety as if each was incorporated by reference individually. 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIG. 1  depicts an illustrative transfection workflow of the invention 
         FIG. 2  depicts an experimental procedure for comparison of electroporation versus a method described herein. 
         FIG. 3  depicts the results of transfection of cells using a method described herein. 
         FIG. 4  depicts the results of transfection of cells using a method described herein. 
         FIG. 5  depicts the results of transfection of PBMCs from two different donors using a method described herein. 
         FIG. 6  is an illustrative computer system to be used with a method described herein. 
         FIG. 7  depicts a workflow that can be used for the reprogramming of stem cells. 
         FIG. 8  illustrates the average fold increase in stem cell colony number using a method described herein. 
         FIG. 9  illustrates the distribution of stem cell colony area using a method described herein. 
         FIG. 10  depicts the cell morphology of cells cultured using a method described herein. 
         FIG. 11  provides the reprogramming kinetics of stem cells cultured using a method described herein. 
         FIG. 12  depicts cardiomyocyte differentiation using a method described herein. 
         FIG. 13  depicts the effect of conditions described herein on stem cell pluripotency and differentiation. 
         FIG. 14  depicts the effect of conditions described herein on stem cell differentiation markers. 
         FIG. 15  depicts immunofluorescence of stem cell markers using a method described herein. 
         FIG. 16  illustrates the average colony area size of differentiated stem cells using a method described herein. 
         FIG. 17  shows the gene expression profile of a population of cells as a function of oxygen concentration and pressure as compared to a standard cell culture incubator. 
         FIG. 18  depicts the change in transfection efficiency with changes in oxygen and pressure levels. 
         FIG. 19  depicts the change in transfection efficiency with changes in oxygen and pressure levels. 
         FIG. 20  provides a workflow for measuring transfection efficiency using a method disclosed herein. 
         FIG. 21  shows the change in transfection efficiency via GFP expression with changes in oxygen and pressure levels. 
         FIG. 22  shows the quantification of transfection efficiency via GFP expression of  FIG. 21  with changes in oxygen and pressure levels. 
         FIG. 23  shows a comparison between the transfection of CD8+ cells enriched from PBMCs and PBMCs with a GFP plasmid using a method described herein. 
         FIG. 24  shows the quantification of the results of  FIG. 23 . 
         FIG. 25  shows that the GFP-transfected CD8+ cells cultured under hypoxic and high pressure conditions developed more multicellular clusters than did cells grown at standard incubator conditions. 
         FIG. 26  shows the percent GFP in the multicellular clusters in cells grown under hypoxic and high pressure conditions compared to cells grown under standard incubator conditions. 
         FIG. 27  shows the quantification of the results of  FIG. 26 . 
         FIG. 28  depicts that when a CRISPR/Cas9 system was used to knockout CTLA4, and knock-in GFP using homology-directed repair, the transfection efficiency of the CRISPR/Cas9 was higher in the cells grown under hypoxic and high pressure conditions than in standard incubator conditions. 
         FIG. 29  shows that the cells grown under hypoxic and high pressure conditions developed a higher percentage of GFP-positive multicellular clusters than the cells grown at standard culture conditions. 
         FIG. 30  shows that the proliferation of the CD8+ cells grown under hypoxic and high pressure conditions was enriched over the cells grown under standard incubator conditions. 
         FIG. 31  depicts a limited dilution assay workflow to assess GFP-positive colonies using the CRISPR/Cas9 system. 
         FIG. 32  shows genome editing of the CD8-positive T-cells as indicated by the GFP signal. 
         FIG. 33  shows that a combination of low oxygen and high pressure enhances ectoderm commitment in defined medium, while causing changes in colony morphology to more mesoderm-like morphology. 
         FIG. 34  shows the change in various stem cell markers upon incubation of cells using a method disclosed herein. 
         FIG. 35  show that different combinations of tumor (disease) extracellular matrix (ECM), low oxygen, and high pressure can alter the gene expression of EGFR and other metabolic regulators in DU145 (prostate cancer) and Panc10 (pancreatic cell lines). 
         FIG. 36  shows that PDL1 expression increased in ARV7-positive, 22RV1 prostate cancer cells during low oxygen and high pressure culturing conditions. 
         FIG. 37  (top panel) provides a western blot showing increased PDL1 protein expression under various conditions of high pressure and hypoxia in both DU145 and 22Rv1 prostate cancer cells. The bottom panel of  FIG. 37  provides a quantification of the western blot results normalized to actin. 
         FIG. 38  shows identification of pressure and oxygen sensitive gene expression signatures in various cell lines. 
         FIG. 39  shows a workflow of taking a biopsy culture taken from a patient having prostate cancer. 
         FIG. 40  shows thst prostate cancer cells were able to form an organoid after two weeks of culture under high pressure and low oxygen conditions. 
         FIG. 41  shows a workflow of taking an apheresis culture taken from a patient having prostate cancer. 
         FIG. 42  shows the mutations found using the COSMIC database from pancreatic ductal adenocarcinoma (PDAC) and circulating tumor cells (CTC) using whole exome sequencing (top panels). 
         FIG. 43  shows that there was increased ex vivo expansion of primary cells under low oxygen and high pressure. 
         FIG. 44  shows that there was increased ex vivo expansion of primary cells under low oxygen and high pressure. 
         FIG. 45  shows the effect that various oxygen and pressure conditions had on the gene expression of immunotherapeutic targets in donor PBMCs. 
         FIG. 46  shows the results of the ex vivo culture and expansion of tumor-infiltrating lymphocytes (TILs) enriched from renal cell carcinoma tumors using high pressure and low conditions. 
         FIG. 47  shows that hypoxic and high pressure conditions can lead to greater enrichment of CD8+ cells from fresh blood samples than culture under standard incubator conditions. 
         FIG. 48  shows an expanded culture time, which indicated that the culture under hypoxic and high pressure conditions generates more CD8+ cells from whole blood than culture under standard conditions. 
         FIG. 49  shows induction of neural precursor markers, PAX6 and NESTIN, in iPSCs after two weeks in culture under 5% O 2  and 2 PSI in stem cell maintenance media. 
         FIG. 50  shows ex vivo cultures of pancreatic ductal adenocarcinoma colonies from a fine-needle aspirate. 
         FIG. 51  shows ex vivo cultures of pancreatic ductal adenocarcinoma colonies from a fine-needle aspirate. 
         FIG. 52  shows ex vivo cultures of pancreatic ductal adenocarcinoma colonies from a fine-needle aspirate. 
         FIG. 53  shows the transfection of human dermal fibroblasts using electroporation of a GFP plasmid. 
         FIG. 54  shows the transfection of PBMCs using electroporation of a GFP plasmid. 
         FIG. 55  shows the transfection of activated CD8+ T-cells using electroporation of a GFP plasmid. 
         FIG. 56  shows the post-transfection effects of CD8+ T-cells using low oxygen and high pressure conditions. 
         FIG. 57  shows a heatmap of the effect on pressure-sensitive genes under various experimental conditions. 
         FIG. 58  shows a heatmap of the effect on oxygen-sensitive genes under various experimental conditions. 
         FIG. 59  is a molecular confirmation of a genome editing experiment. 
         FIG. 60  is a molecular confirmation of a genome editing experiment. 
     
    
    
     DETAILED DESCRIPTION 
     Transfection. 
     A method described herein can be used to increase, for example, transfection and transduction efficiency in cells. Transduction can be used, for example, to introduce a viral vector in a cell. Viral nucleic acid delivery systems can use recombinant viruses to deliver nucleic acids for gene therapy. Non-limiting examples of viruses that can be used to deliver nucleic acids include retrovirus, adenovirus, herpes simplex virus, adeno-associated virus, vesicular stomatitis virus, reovirus, vaccinia, pox virus, lentivirus, and measles virus. 
     Transfection methods that can be used with methods of the invention include, for example, lipofection, electroporation, calcium phosphate transfection, chemical transfection, polymer transfection, gene gun, magnetofection, or sonoporation.  FIG. 1  depicts an illustrative transfection workflow of the invention.  FIG. 1  shows the transfection of, for example, DU145 (human prostate cancer), LnCaP (androgen-sensitive human prostate adenocarcinoma), U87 (human primary glioblastoma), PANC10 (pancreatic adenocarcinoma), or PBMCs (peripheral blood mononuclear cells) with a GFP (green fluorescent protein) plasmid. The cells can be cultured in hypoxic conditions, for example, at 1% or 5% oxygen, and at conditions that are about 2 PSI greater or less than normal pressure conditions. The transfection allows introduction of the GFP-expressing plasmid into the cell. 
     Viral nucleic acid delivery methods can use recombinant viruses for nucleic acid transfer. Non-viral nucleic acid delivery can comprise injecting naked DNA or RNA, use of carriers including lipid carriers, polymer carriers, chemical carriers and biological carriers such as biologic membranes, bacteria, and virus-like particles, and physical/mechanical approaches. A combination of viral and non-viral nucleic acid delivery methods can be used for efficient gene therapy. 
     Non-viral nucleic acid transfer can include injection of naked nucleic acid, for example, nucleic acid that is not protected or devoid of a carrier. Hydrodynamic injection methods can increase the targeting ability of naked nucleic acids. 
     Non-viral nucleic acid delivery systems can include chemical carriers. These systems can include lipoplexes, polyplexes, dendrimers, and inorganic nanoparticles. A lipoplex is a complex of a lipid and a nucleic-acid that protects the nucleic acid from degradation and facilitates entry into cells, and can be prepared from neutral, anionic, or cationic lipids. Lipoplexes can enter cells by endocytosis, and release the nucleic acid contents into the cytoplasm. A polyplex is a complex of a polymer and a nucleic acid, and are prepared from cationic polymers that facilitate assembly by ionic interactions between nucleic acids and polymers. Uptake of polyplexes into cells can occur by endocytosis. Inside the cells, polyplexes require co-transfected endosomal rupture agents such as inactivated adenovirus, for the release of the polyplex particle from the endocytic vesicle. Examples of polymeric carriers include polyethyleneimine, chitosan, poly(beta-amino esters) and polyphosphoramidate. Dendrimers can be constructed to have a positively-charged surface and/or carry functional groups that aid temporary association of the dendrimer with nucleic acids. These dendrimer-nucleic acid complexes can be used for gene therapy. The dendrimer-nucleic acid complex can enter the cell by endocytosis. Nanoparticles prepared from inorganic material can be used for nucleic acid delivery. Examples of inorganic material can include gold, silica/silicate, silver, iron oxide, and calcium phosphate. Inorganic nanoparticles with a size of less than 100 nm can be used to encapsulate nucleic acids efficiently. The nanoparticles can be taken up by the cell via endocytosis, and the nucleic acid can be released from the endosome without degradation. Nanoparticles based on quantum dots can be prepared and offers the use of a stable fluorescence marker coupled with gene therapy. Organically modified silica or silicate can be used to target nucleic acids to specific cells in an organism. 
     Non-viral nucleic acid delivery systems can include biological methods including bactofection, biological liposomes, and virus-like particles (VLPs). The bactofection method comprises using attenuated bacteria to deliver nucleic acids to a cell. Biological liposomes, such as erythrocyte ghosts and secretion exosomes, are derived from the subject receiving gene therapy to avoid an immune response. Virus-like particles (VLP) or empty viral particles are produced by transfecting cells with only the structural genes of a virus and harvesting the empty particles. The empty particles are loaded with nucleic acids to be transfected for gene therapy. 
     Examples of physical methods of transfection include electroporation, gene gun, sonoporation, and magnetofection. The electroporation method uses short high-voltage pulses to transfer nucleic acid across the cell membrane. These pulses can lead to formation of temporary pores in the cell membrane, thereby allowing nucleic acid to enter the cell. Electroporation can be efficient for a broad range of cells. Electron-avalanche transfection is a type of electroporation method that uses very short, for example, microsecond, pulses of high-voltage plasma discharge for increasing efficiency of nucleic acid delivery. The gene gun method utilizes nucleic acid-coated gold particles that are shot into the cell using high-pressure gas. Force generated by the gene gun allows penetration of nucleic acid into the cells, while the gold is left behind on a stopping disk. The sonoporation method uses ultrasonic frequencies to modify permeability of cell membrane. Change in permeability allows uptake of nucleic acid into cells. The magnetofection method uses a magnetic field to enhance nucleic acid uptake. In this method, nucleic acid is complexed with magnetic particles. A magnetic field is used to concentrate the nucleic acid complex and bring them in contact with cells. 
     Non-limiting examples of viruses that can be used to deliver nucleic acids include retrovirus, adenovirus, herpes simplex virus, adeno-associated virus, vesicular stomatitis virus, reovirus, vaccinia, pox virus, and measles virus. 
     Non-limiting examples of retroviral vectors include Moloney murine leukemia viral (MMLV) vectors, HIV-based viral vectors, gammaretroviral vectors, C-type retroviral vectors, and lentiviral vectors. Lentivirus is a subclass of retrovirus. While some retroviruses can infect only dividing cells, lentiviruses can infect and integrate into the genome of actively dividing cells and non-dividing cells. 
     An adenovirus is a non-enveloped virus with a linear double-stranded genome. Adenoviruses can enter host cells using interactions between viral surface proteins and host cell receptors that lead to endocytosis of the adenovirus particle. Once inside the host cell cytoplasm, the adenovirus particle is released by the degradation of the endosome. Using cellular microtubules, the adenovirus particle gains entry into the host cell nucleus, where adenoviral DNA is released. Inside the host cell nucleus, the adenoviral DNA is transcribed and translated, without integrating into the host cell genome. 
     Herpes simplex virus (HSV)-based vectors can be used in the disclosure. The HSV is an enveloped virus with a linear double-stranded DNA genome. Interactions between surface proteins on the host cell and HSV lead to pore formation in the host cell membrane. These pores allow HSV to enter the host cell cytoplasm, and once inside the host cell, the HSV uses the nuclear entry pore to enter the host cell nucleus where HSV DNA is released. HSV can persist in host cells in a state of latency. Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), also known as human herpes virus 1 and 2 (HHV-1 and HHV-2), are members of the herpes virus family. 
     Alphavirus-based vectors can be used to deliver nucleic acids. Examples of alphavirus-based vectors include vectors derived from semliki forest virus and sindbis virus. 
     Pox/vaccinia-based vectors such as orthopox or avipox vectors can be used in the present invention. Pox virus is a double stranded DNA virus that can infect diving and non-dividing cells. Pox viral genome can accommodate up to 25 kb transgenic sequence. Multiple genes can be delivered using a single vaccinia viral vector. 
     Adeno-associated virus (AAV) is a small, non-enveloped virus that belongs to the Parvoviridae family. The AAV genome is a linear single-stranded DNA molecule of about 4,800 nucleotides. The AAV DNA comprises two inverted terminal repeats (ITRs) at both ends of the genome and two sets of open reading frames. The ITRs serve as origins of replication for the viral DNA and as integration elements. The open reading frames encode for the Rep (non-structural replication) and Cap (structural capsid) proteins. AAV can infect dividing cells and quiescent cells. AAV can be engineered for use as a gene therapy vector by substituting the coding sequence for both AAV genes with a transgene (transferred nucleic acid) to be delivered to a cell. The substitution eliminates immunologic or toxic side effects due to expression of viral genes. The transgene can be placed between the two ITRs (145 bp) on the AAV DNA molecule. 
     A pseudotyped virus can be used for the delivery of nucleic acids. Pseudotyping involves substitution of endogenous envelope proteins of the virus by envelope proteins from other viruses or chimeric proteins. The foreign envelope proteins can confer a change in host tropism or alter stability of the virus. An example of a pseudotyped virus useful for gene therapy includes vesicular stomatitis virus G-pseudotyped lentivirus (VSV G-pseudotyped lentivirus) that is produced by coating the lentivirus with the envelope G-protein from Vesicular stomatitis virus. VSV G-pseudotyped lentivirus can transduce almost all mammalian cell types. 
     A hybrid vector having properties of two or more vectors can be used for nucleic acid delivery to a host cell. Hybrid vectors can be engineered to reduce toxicity or improve therapeutic transgene expression in target cells. Non-limiting examples of hybrid vectors include AAV/adenovirus hybrid vectors, AAV/phage hybrid vectors, and retrovirus/adenovirus hybrid vectors. 
     A viral vector can be replication-competent. A replication-competent vector contains all the genes necessary for replication, making the genome lengthier than replication-defective viral vectors. A viral vector can be replication-defective, wherein the coding region for the genes essential for replication and packaging are deleted or replaced with other genes. Replication-defective viruses can transduce host cells and transfer the genetic material, but do not replicate. A helper virus can be supplied to help a replication-defective virus replicate. 
     A viral vector can be derived from any source, for example, humans, non-human primates, dogs, fowl, mouse, cat, sheep, and pig. 
     The nucleic acid of the disclosure can be generated using any method. The nucleic acid can be synthetic, recombinant, isolated, and/or purified. 
     A vector of the present disclosure can comprise one or more types of nucleic acids. The nucleic acids can include DNA or RNA. RNA nucleic acids can include a transcript of a gene of interest. DNA nucleic acids can include the gene of interest, promoter sequences, untranslated regions, and termination sequences. A combination of DNA and RNA can be used. The nucleic acids can be double-stranded or single-stranded. The nucleic acid can include non-natural or altered nucleotides. 
     A vector of the disclosure can comprise nucleic acids encoding a selectable marker. The selectable marker can be positive, negative or bifunctional. The selectable marker can be an antibiotic-resistance gene. Examples of antibiotic resistance genes include markers conferring resistance to kanamycin, gentamicin, ampicillin, chloramphenicol, tetracycline, doxycycline, hygromycin, puromycin, zeomycin, or blasticidin. The selectable marker can allow imaging of the host cells, for example, a fluorescent protein. Examples of imaging marker genes include GFP, eGFP, RFP, CFP, YFP, dsRed, Venus, mCherry, mTomato, and mOrange. 
     The transfection can be a stable or transient transfection. The transfection can be used to transfect DNA plasmids, RNA, siRNA, shRNA, or any nucleic acid. The plasmids can encode, for example, green fluorescent protein (GFP), selectable markers, and other proteins of interest. The selectable markers can provide resistance to, for example, G418, hygromycin B, puromycin, and blasticidin. 
     A Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)—CRISPR associated (Cas) (CRISPR-Cas) system can be used to modify a target or deliver a nucleic acid of the disclosure. The CRIPSR-Cas system is a targeted genome-editing system comprising a Cas nuclease that is guided to specific DNA sequences, for example, a genomic locus in a subject, by a guide RNA molecule. The Cas nuclease can modify the genomic locus, for example, by cleaving the genomic locus, thus generating mutations that result in loss of function of the target sequence. The Cas nuclease can also modify the genomic locus, for example, by cleaving the genomic locus, and adding a transgene, for example, a therapeutic nucleic acid of the disclosure. The CRIPSR/Cas system can be used in conjunction with other nucleic acid delivery methods such as viral vectors and non-viral methods as described herein. 
     A CRISPR interference (CRISPRi) system can be used to modify the expression of a target of the disclosure. The CRISPRi system is a targeted gene regulatory system comprising a nuclease deficient Cas enzyme fused to a transcriptional regulatory domain that is guided to specific DNA sequences, for example, a genomic locus in a subject, by a guide RNA molecule. The Cas/regulator fusion protein can occupy the genomic locus and induce, for example, transcriptional repression of the target gene through the function of a negative regulatory domain fused to the Cas protein. The CRISPRi system can be used in conjunction with other nucleic acid delivery methods such as viral vectors and non-viral methods as described herein. 
     A method of the invention can increase the transfection or transduction efficiency by, for example, about 2-fold, about 3-fold, about 4-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 12-fold, about 14-fold, about 16-fold, about 18-fold, about 20-fold, about 25-fold, about 30-fold, about 35-fold, about 40-fold, about 45-fold, about 50-fold, about 60-fold, about 70-fold, about 80-fold, about 90-fold, or about 100-fold. 
     In some embodiments, a hypoxic or positive pressure condition is applied to a cell prior to transfection. In some embodiments, a hypoxic or positive pressure condition is applied to a cell after transfection. A method described herein can comprise a conditioning step, where the conditioning step is for 24-48 hours and comprises culturing the cell to be transfected in a hypoxic or high pressure condition prior to the transfection. A method described herein can comprise a recovery period, where the recovery period comprises culturing a cell post-transfection in a hypoxic or positive pressure condition. In some embodiments, a transfection method described herein comprises a conditioning step, where the conditioning step comprises culturing the cell prior to transfection in a hypoxic or positive pressure condition for 24-48 hours. In some embodiments, a transfection method described herein comprises a recovery period, where the recovery period comprises culturing the cell after transfection in a hypoxic or positive pressure condition. In some embodiments, a transfection method described herein comprises both a conditioning step and a recovery period. 
     In some embodiments, a conditioning step prior to transfection can use moderate oxygen and moderate pressure levels to efficiently propagate cells while maintaining, for example, pluripotency. The oxygen levels can vary from about 5% to about 15%. Pressure levels can vary from about 0.1 PSI to about 2 PSI. 
     In some embodiments, a recovery phase after a transfection can use low oxygen and high pressure levels to increase transfection and recovery of cells by increasing cell viability. The oxygen levels can vary from about 0.1% to about 2%. Pressure levels can vary from about 2 PSI to about 5 PSI. 
     In some embodiments, positive pressure is used to increase transfection efficiency. In some embodiments, hypoxia is used to increase transfection efficiency. In some embodiments, hypoxia and positive pressure are used to increase transfection efficiency. 
     Stem Cells. 
     A method disclosed herein can be used to reprogram, for example, fibroblasts to pluripotent stem cells. A method disclosed herein can, for example, increase the efficiency and increase the rate of cell reprogramming. A method disclosed herein can further increase, for example, the number and size of stem cell colonies that form as a result of the reprogramming protocol. The cells can be reprogrammed into, for example, totipotent, pluripotent, multipotent, oligopotent, or unipotent stem cells. 
     Reprogramming of cells into pluripotent stem cells can be enhanced by, for example, culturing the cells under hypoxic and positive pressure conditions. The cells can be reprogrammed by transfecting cells with, for example, an RNA replicon vector encoding several stem cell transformation factors. The stem cell transformation factors can include, for example, Oct4, Sox2, KLF-4, GLIS1, and c-MYC. Additional stem cell transformation factors include, for example, Nanog and Lin28. After transfection of the cells with the reprogramming factors, the cells can be maintained in media designed to differentiate and maintain stem cell populations. The cells can be grown under hypoxic and high pressure conditions as disclosed herein to induce differentiation of the cells. 
     Adult stem cells can be found in many organs and tissues including, for example, brain, bone marrow, peripheral blood, blood vessels, skeletal muscle, skin, teeth, heart, gut, liver, ovarian epithelium, and testis. The stem cells can reside in stem cell niches within the various areas of the body. In many tissues, some types of stem cells are pericytes, which are cells that compose the outermost layer of small blood vessels. Stem cells may remain quiescent non-dividing for long periods of time until they are activated by a normal need for more cells to maintain tissues, or by disease or tissue injury. 
     Markers that can be used to identify iPSCs include, for example, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, TRA-2-49/6E, Nanog, Oct3/4, Sox2, GDF3, REX1, FGF4, ESG1, DPPA2, DPPA4, and hTERT. 
     The iPSCs can be induced to differentiate into, for example, neuronal cells, hippocampal progenitors, dentate granule cell neurons, MGE progenitors, cortical interneurons, dorsal cortical progenitors, excitatory cortical neurons, glial progenitors, astrocytes, neural crest stem cells, dopaminergic neurons, oligodendrocytes, dopaminergic neurons, hematopoietic cells, B-cells, T-cells, NK cells, granulocytes, monocytes, macrophages, erythrocytes, megakaryocytes, platelets, cardiomyocytes, hepatocytes, skeletal muscle cells, adipocytes, pancreatic beta-cells, or cells from the ectoderm, mesoderm, or endoderm. 
     The stem cells obtained using a method disclosed herein can be cultured on, for example, a gelatin-coated culture dish. The cells can be in cultured in medium containing inactivated mouse embryonic fibroblast (MEF) medium, basic FGF solution, pluripotent culture medium, leukemia inhibitory factor, and a collagenase solution. The stem cells can additionally be grown over a layer of feeder cells, which can be, for example, MEFs, JK1 cells, or SNL 76/7 cells. 
     Expression markers that can be measured to assess the differentiation or gene expression profile of an initial cell culture to iPSCS can include, for example, IGF1, CTNNB1, AXIN1, KAT2A, CD4, CXCL12, FZD9, CD44, ACTC1, JAG1, BMP1, FZD2, IL6ST, FZD7, LIFR, SMAD4, DVL1, CTNNA1, FGFR1, WNT1, PPARG, COL1A1, FGF1, GLL, DNMT3B, PSEN1, ALDH1A1, JUND, SDAD1, NCSTN, FZD6, TCF7, NOTCH1, APC, RB1, NUMB, CREBBP, GATA6, PSEN2, HDAC2, CCND1, CCNE1, EP300, Notch2, MME, GLI2, BTRC, STAT3, PPARD, Notch3, Notch4, GLI3, CDC42, CCNA2, ISL1, BMP2, PAX6, S100B, CD3D, FZD5, Nanog, CDH1, Sox1, DLL1, CCND2, SMO, COL2AI, LIFR, or COX2. 
     Plant Cells. 
     A method disclosed herein can be used to genetically engineer or to reprogram plant cells. A method disclosed herein can be used to create plant cells with a particular genotype that alters the cell&#39;s ability to produce a specific molecule or that results in a specific phenotype. Some embodiments of the invention comprise modulating local pressure and oxygen conditions during transformation of plant cells. 
     A method disclosed herein can be applied to any type of plant cell or tissue. Plant cells or tissues used in the invention can include roots, leaves, monocotyledons such as cotton, soybean,  Brassica , and peanut, dicotyledons such as asparagus, barley, maize, oat, rice, sugarcane, tall fescue, and wheat, hypocotyl tissue, callus tissue, nodal explants, shoot meristem, cell cultures, immature embryos, scutellar tissue, and immature inflorescence. 
     In addition to or in conjunction with the methods described herein, the invention can include the use of  Agrobacterium  tumor-inducing (Ti) plasmid genes, which can contain a transfer DNA region (T-DNA), for engineering a plant cell&#39;s DNA.  Agrobacterium  can be used in the invention to produce Ti plasmid genes, and  Agrobacterium  strains used in the invention can include  Agrobacterium tumefaciens  strain C58, nopaline strains, octopine strains such as LBA4404, and agropine strains such as EHA101, EHA105, and EHA 109. 
     The invention can also include the use of promoters such as nopaline synthase (NOS) promoter, octopine synthase (OCS) promoter, caulimovirus promoters such as cauliflower mosaic virus (CaMV) 19S and 35S promoters, enhanced CaMV 35S promoter (e35S), figwort mosaic virus (FMV) 35S promoter, and promoters from the ribulose bisphosphate carboxylase (Rubisco) family such as Rubisco small subunit and Rubisco activase promoters in engineered plant cells. 
     Conditions Used in Methods Disclosed Herein. 
     The present invention can use a substrate to culture the cells during transfection. The cells can be applied to, for example, a culture dish coated with a substrate that can promote growth and enrichment of the cells. Cells that do not adhere to the substrate can be washed away with media. Once adhered, the cells can spread and begin dividing on the substrate. 
     The substrate can comprise, for example, 1, 2, 3, 4, or 5 layers. The distance between two substrates layers may range from about 0.1 to about 20 mm, about 1 to about 10 mm, or about 1 to about 5 mm and each layer can be about 0.1, about 1, about 2, about 3, about 4, about 5, about 6, about 7, about 8, about 9, about 10, about 12, about 15, about 17, or about 20 mm. 
     The cells can be plated on a material made of, for example, plastic, glass, gelatin, polyacrylamide, or any combination thereof. The dishes used to the plate the cells can be, for example, microscope slides, culture plates, culture dishes, Petri dishes, microscope coverslips, an enclosed environmental chamber, a sealed culture dish, or multi-well culture dishes. 
     The binding surface layer of the substrate can be the portion of the substrate that is in contact with the cells. In some instances, the binding surface layer is the only layer, adjacent to the base layer, or separated from the base layer by one or more middle layers. 
     The binding surface layer of the substrate can comprise, for example, cell monolayers, cell lysates, biological materials associated with the extracellular matrix (ECM), gelatin, or any combination thereof. 
     Biological materials associated with the ECM can include, for example, collagen type I, collagen type IV, laminin, fibronectin, elastin, reticulin, hygroscopic molecules, glycosaminoglycanse, roteoglycans, glycocalyx, bovine serum albumin, Poly-L-lysine, Poly-D-lysine, or Poly-L-ornithine. The gelatin can be from an animal source, for example, the gelatin can porcine or bovine. 
     The monolayer of cells used in the substrate can be, for example, mammalian cells, endothelial cells, vascular cells, venous cells, capillary cells, human umbilical vein endothelial cells (HUVEC), human lung microvascular endothelial cells (HLMVEC). The cell lines can be obtained from a primary source or from an immortalized cell line. The monolayer of cells can be irradiated by ultraviolet light or X-ray sources to cause senescence of cells. The monolayer can also contain a mixture of one or more different cell types. The different cell types may be co-cultured together. One non-limiting example of co-culture is a combination of primary human endothelial cells co-cultured with transgenic mouse embryonic fibroblasts mixed to form a monolayer. 
     The binding surface layer of the substrate can contain, for example, a mixture of intracellular components. One method that can be used to obtain a mixture of intracellular components is lysis of the cells and collection of the cytosolic components. The lysed cells can be primary or immortalized. The lysed cells can be from either mono- or co-cultures. 
     The binding surface layer of the substrate can contain biological materials associated with the extracellular matrix (ECM) or binding moieties. For example, gelatin can be mixed directly with cells, binding moieties, biological materials associated with the ECM, or any combination thereof, to make a binding surface layer for the substrate. For example, the binding surface layer can be comprised of a gelatin mixed with a collagen. 
     The substrate can have one or more middle layers. The middle layer of the substrate can be one or more monolayers of cells. The cells of the monolayer can be of varying origin. For example, the middle layer of the substrate can be made by growing a confluent monolayer of mouse embryonic fibroblasts on the base layer and then growing another layer of cells, for example, the binding surface layer, on top of the confluent mouse embryonic fibroblasts. 
     A feeder layer can be used in the substrate for growth or reprogramming of the cells. A feeder layer can sit adjacent to a base layer and can be separated from the binding surface layer of the substrate. The feeder layer can be a monolayer of feeder cells. The cells of the monolayer can be of varying origin. For example, the feeder layer can be made by growing a monolayer of human endothelial cells or mouse embryonic fibroblasts on a base layer. 
     Conjugation of layers of the substrate can be done by allowing cells to grow in a monolayer on top of the base layer or middle layer. Conjugation of layers can also be done by pre-treating the surface with a surface of either net positive, net negative, or net neutral charge. The conjugation procedure can be aided by chemical moieties, linkers, protein fragments, nucleotide fragments, or any combination thereof. 
     The media used for growing the cells can be supplemented or made with culture media that has been collected from cell cultures, blood plasma, or any combination thereof. The enrichment media can be, for example, Plating Culture Medium, Type R Long Term Growth Medium, Type DF Long Term Growth Medium, Type D Long Term Growth Medium, and MEF—Enrichment Medium, or any combination thereof. The enrichment medium can contain, for example, a primary nutrient source, animal serum, ions, elements, calcium, glutamate, magnesium, zinc, iron, potassium, sodium, amino acids, vitamins, glucose, growth factors, hormones, tissue extracts, proteins, small molecules, or any combination thereof. In some embodiments, the culture media used for transfection does not contain serum. 
     Non-limiting examples of amino acids include essential amino acids, phenylalanine, valine, threonine, tryptophan, isoleucine, methionine, leucine, lysine, and histidine, arginine, cysteine, glycine, glutamine, proline, serine, tyrosine, alanine, asparagine, aspartic acid, glutamic acid, or any combination thereof. 
     Non-limiting examples of growth factors include Epidermal Growth Factor (EGF), Nerve Growth Factor (NGF), Brain Derived Neurotrophic Factor (BDNF), Fibroblast Growth Factor (FGF), Stem Cell Factor (SCF), Insulin-like Growth Factor (IGF), Transforming Growth Factor-beta (TGF-β), or any combination thereof. 
     Non-limiting examples of hormones include peptide hormones, insulin, steroidal hormones, hydrocortisone, progesterone, testosterone, estrogen, dihydrotestosterone, or any combination thereof. 
     Non-limiting examples of tissue extracts include pituitary extract. Non-limiting examples of small molecule additives include sodium pyruvate, endothelin-1, transferrin, cholesterol, or any combination thereof. 
     The culturing conditions in a method of the invention can be adjusted to simulate oxygen and pressure levels found, for example, in pathological conditions. The oxygen level used during culturing conditions can be, for example, about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, about 10%, about 11%, about 12%, about 13%, about 14%, about 15%, about 16%, about 17%, about 18%, about 19%, about 20%, about 21%, about 22%, about 23%, about 24%, or about 25% oxygen in the incubator. In some embodiments, the cells can be grown under hypoxic conditions during transfection. 
     The culturing condition in a method of the invention can be adjusted to simulate the pressure found, for example, in pathological conditions. The pressure used during culturing conditions can be about 0 PSI, about 0.1 PSI, about 0.15 PSI about 0.2 PSI, about 0.25 PSI, about 0.3 PSI, about 0.35 PSI, about 0.4 PSI, about 0.45 PSI, 0.5 PSI, about 0.55 PSI, about 0.6 PSI, about 0.65 PSI, about 0.7 PSI, about 0.75 PSI, about 0.8 PSI, about 0.85 PSI, about 0.9 PSI, about 0.95 PSI, about 1 PSI, about 1.1 PSI, about 1.2 PSI, about 1.3 PSI, about 1.4 PSI, about 1.5 PSI, about 1.6 PSI, about 1.7 PSI, about 1.8 PSIG, about 1.9 PSI, about 2 PSI, about 2.1 PSI, about 2.2 PSI, about 2.3 PSI, about 2.4 PSI, about 2.5 PSI, about 2.6 PSI, about 2.7 PSI, about 2.8 PSI, about 2.9 PSI, about 3 PSI, about 3.5 PSI, about 4 PSI, about 4.5 PSI, about 5 PSI, about 6 PSI, about 7 PSI, about 8 PSI, about 9 PSI, or about 10 PSI. A pressure used in a method disclosed herein can be an above atmospheric pressure value. A pressure used in a method disclosed herein can be positive pressure. 
     The culturing condition in a method of the invention can be adjusted to simulate the pressure found, for example, in pathological conditions. The pressure used during culturing conditions can be a PSI gauge (PSIG) reading of, for example, about 0.5 PSIG, about 0.6 PSIG, about 0.7 PSIG, about 0.8 PSIG, about 0.9 PSIG, about 1 PSIG, about 1.1 PSIG, about 1.2 PSIG, about 1.3 PSIG, about 1.4 PSIG, about 1.5 PSIG, about 1.6 PSIG, about 1.7 PSIG, about 1.8 PSIG, about 1.9 PSIG, about 2 PSIG, about 2.5 PSIG, about 3 PSIG, about 3.5 PSIG, about 4 PSIG, about 4.5 PSIG, about 5 PSIG, about 6 PSIG, about 7 PSIG, about 8 PSIG, about 9 PSIG, about 10 PSIG, about 15 PSIG, about 20 PSIG, about 25 PSIG, about 30 PSIG, about 35 PSIG, about 40 PSIG, about 45 PSIG, about 50 PSIG, or about 55 PSIG. 
     The pressure used during culturing conditions can be, for example, about 3.45 kPa, about 4.14 kPa, about 4.83 kPa, about 5.52 kPa, about 6.21 kPa, about 6.89 kPa, about 7.58 kPa, about 8.27 kPa, about 8.96 kPa, about 9.65 kPa, about 10.3 kPa, about 11 kPa, about 11.7 kPa, about 12.4 kPa, about 13.1 kPa, about 13.8 kPa, about 17.2 kPa, about 20.7 kPa, about 24.1 kPa, about 27.6 kPa, about 31 kPa, about 34.4 kPa, about 41.4 kPa, about 48.3 kPa, about 55.2 kPa, about 62.1 kPa, about 68.9 kPa, about 103 kPa, about 138 kPa, about 172 kPa, about 207 kPa, about 241 kPa, about 276 kPa, about 310 kPa, about 345 kPa, or about 379 kPa. 
     The pressure used in a method of the invention can be delivered continuously or via pulses of pressure produced by repeated depressurizations and pressurizations of an incubator used in the method. The pulses of pressure can be separated by, for example, about 1 minute, about 1.5 minutes, about 2 minutes, about 2.5 minutes, about 3 minutes, about 3.5 minutes, about 4 minutes, about 4.5 minutes, about 5 minutes, about 6 minutes, about 7 minutes, about 8 minutes, about 9 minutes, about 10 minutes, about 11 minutes, about 12 minutes, about 13 minutes, about 14 minutes, about 15 minutes, about 16 minutes, about 17 minutes, about 18 minutes, about 19 minutes, or about 20 minutes, about 21 minutes, about 22 minutes, about 23 minutes, about 24 minutes, about 25 minutes, about 26 minutes, about 27 minutes, about 28 minutes, about 29 minutes, about 30 minutes, about 32 minutes, about 34 minutes, about 36 minutes, about 38 minutes, about 40 minutes, about 45 minutes, about 50 minutes, about 55 minutes, about 1 hour, about 1.5 hours, about 2 hours, about 2.5 hours, about 3 hours, about 3.5 hours, about 4 hours, about 4.5 hours, or about 5 hours. 
     The pH of the media used in a method of the invention can be, for example, about 2, about 2.1, about 2.2, about 2.3, about 2.4, about 2.5, about 2.6, about 2.7, about 2.8, about 2.9, about 3, about 3.1, about 3.2, about 3.3, about 3.4, about 3.5, about 3.6, about 3.7, about 3.8, about 3.9, about 4, about 4.1, about 4.2, about 4.3, about 4.4, about 4.5, about 4.55, about 4.6, about 4.7, about 4.8, about 4.9, about 5, about 5.5, about 6, about 6.5, about 6.6, about 6.7, about 6.8, about 6.9, about 7, about 7.1, about 7.2, about 7.3, about 7.4, about 7.5, about 7.6, about 7.7, about 7.8, about 7.9, about 8, about 8.5, about 9, about 9.5, about 10, about 10.5, or about 11 pH units. 
     The viscosity of the media can be adjusted by, for example, at least 0.001 Pascal-second (Pa·s), at least 0.001 Pa·s, at least 0.0009 Pa·s, at least 0.0008 Pa·s, at least 0.0007 Pa·s, at least 0.0006 Pa·s, at least 0.0005 Pa·s, at least 0.0004 Pa·s, at least 0.0003 Pa·s, at least 0.0002 Pa·s, at least 0.0001 Pa·s, at least 0.00005 Pa·s, or at least 0.00001 Pa·s depending on the cell types being cultured. 
     The oxygen solubility of the media can be, for example, about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, about 10%, about 11%, about 12%, about 13%, about 14%, about 15%, about 16%, about 17%, about 18%, about 19%, about 20%, about 21%, about 22%, about 23%, about 24%, about 25%, about 26%, about 27%, about 28%, about 29%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 96%, about 97%, about 98%, or about 99%. 
     In some embodiments, a culture media used for a method described herein can contain, for example, an L-alanine-L-glutamine dipeptide, B27 TM supplement, human bFGF, human EGF, human HGH, 1 mg/mL human insulin, 0.55 mg/mL human transferrin, 0.5 μg/mL sodium selenite, beta-mercaptoethanol, non-essential amina acids solution, and high glucose media. 
     In some embodiments, for PBMC or CD8+ cell culture, a culture media for a method described herein can contain, for example, PHA-P (10 μg/mL), IL-2 (100 U/mL), IL-4 (20 ng/mL), IL-15 (100 ng/mL), GM-CSF (20 ng/mL), and LPS (100 ng/mL). 
     The oxygen concentration used in a method disclosed herein can be used to mimic oxygen concentration found in, for example, solid tumors (about 1.1%), muscle (about 3.8%), prostate (about 3.9%), brain (about 4.4%), peripheral tissues (about 5.3%), venous blood (about 5.3%), lung (about 5.6%), bone marrow (about 6.4%), intestinal tissue (about 7.6%), kidney (about 9.5%), and arterial blood (about 13.2%). 
     The pressure conditions used in a method disclosed herein can be used to mimic the interstitial fluid pressure found in, for example, normal breast (about 0.02 PSI), normal skin (about 0.04 PSI), lymphoma (about 0.14 PSI), brain tumors (about 0.15 PSI), sarcoma (about 0.17 PSI), lung carcinoma (about 0.25 PSI), rectal carcinoma (about 0.33 PSI), breast carcinoma (about 0.37 PSI), head and neck carcinoma (about 0.41 PSI), metastatic melanoma (about 0.43 PSI), colorectal carcinoma liver metastases (about 0.43 PSI), cervical carcinoma (about 0.44 PSI), ovarian carcinoma (about 0.48 PSI), and renal cell carcinoma (about 0.72 PSI). 
     Therapeutic Uses. 
     Subjects can be, for example, elderly adults, adults, adolescents, pre-adolescents, children, toddlers, infants. Subjects can be non-human animals, for example, a subject can be a mouse, rat, cow, horse, donkey, pig, sheep, dog, cat, or goat. A subject can be a patient. 
     A method disclosed herein can be used to identify a therapeutic, a biomarker, a genetic mutation, or a therapeutic target for, for example, stem cell differentiation or differentiation of various cell types. 
     Genomic, proteomic, and metabolic analysis can be conducted on the transfected cells to, for example, identify biomarkers that can be used for development of cancer therapies, drug development, cancer vaccines, cancer screening, diagnostics, personalized antibody development, hematopoietic stem cell transplantation, organ transplantation, or cardiovascular disease treatment. A method described herein can be used to induce phenotypic and genotypic changes in cells to determine the effect of cancer therapies. The cancer therapies can include, for example, chemotherapeutics or gene therapy. 
     Non-limiting examples of cancers that can be analyzed in a method disclosed herein include: acute lymphoblastic leukemia, acute myeloid leukemia, adrenocortical carcinoma, AIDS-related cancers, AIDS-related lymphoma, anal cancer, appendix cancer, astrocytomas, basal cell carcinoma, bile duct cancer, bladder cancer, bone cancers, brain tumors, such as cerebellar astrocytoma, cerebral astrocytoma/malignant glioma, ependymoma, medulloblastoma, supratentorial primitive neuroectodermal tumors, visual pathway and hypothalamic glioma, breast cancer, bronchial adenomas, Burkitt lymphoma, carcinoma of unknown primary origin, central nervous system lymphoma, cerebellar astrocytoma, cervical cancer, childhood cancers, chronic lymphocytic leukemia, chronic myelogenous leukemia, chronic myeloproliferative disorders, colon cancer, cutaneous T-cell lymphoma, desmoplastic small round cell tumor, endometrial cancer, ependymoma, esophageal cancer, Ewing&#39;s sarcoma, germ cell tumors, gallbladder cancer, gastric cancer, gastrointestinal carcinoid tumor, gastrointestinal stromal tumor, gliomas, hairy cell leukemia, head and neck cancer, heart cancer, hepatocellular (liver) cancer, Hodgkin lymphoma, Hypopharyngeal cancer, intraocular melanoma, islet cell carcinoma, Kaposi sarcoma, kidney cancer, laryngeal cancer, lip and oral cavity cancer, liposarcoma, liver cancer, lung cancers, such as non-small cell and small cell lung cancer, lymphomas, leukemias, macroglobulinemia, malignant fibrous histiocytoma of bone/osteosarcoma, medulloblastoma, melanomas, mesothelioma, metastatic squamous neck cancer with occult primary, mouth cancer, multiple endocrine neoplasia syndrome, myelodysplastic syndromes, myeloid leukemia, nasal cavity and paranasal sinus cancer, nasopharyngeal carcinoma, neuroblastoma, non-Hodgkin lymphoma, non-small cell lung cancer, oral cancer, oropharyngeal cancer, osteosarcoma/malignant fibrous histiocytoma of bone, ovarian cancer, ovarian epithelial cancer, ovarian germ cell tumor, pancreatic cancer, pancreatic cancer islet cell, paranasal sinus and nasal cavity cancer, parathyroid cancer, penile cancer, pharyngeal cancer, pheochromocytoma, pineal astrocytoma, pineal germinoma, pituitary adenoma, pleuropulmonary blastoma, plasma cell neoplasia, primary central nervous system lymphoma, prostate cancer, rectal cancer, renal cell carcinoma, renal pelvis and ureter transitional cell cancer, retinoblastoma, rhabdomyo sarcoma, salivary gland cancer, sarcomas, skin cancers, skin carcinoma merkel cell, small intestine cancer, soft tissue sarcoma, squamous cell carcinoma, stomach cancer, T-cell lymphoma, throat cancer, thymoma, thymic carcinoma, thyroid cancer, trophoblastic tumor (gestational), cancers of unknown primary site, urethral cancer, uterine sarcoma, vaginal cancer, vulvar cancer, Waldenström macroglobulinemia, and Wilms tumor. 
     Methods that can be used to determine the presence of, for example, biological markers or transfection of desired genes can include, for example, qPCR, RT-PCR, immunofluorescence, immunohistochemistry, western blotting, high-throughput sequencing, or mRNA sequencing. 
     Computer Systems. 
     A method of the invention can be used to, for example, sequence, image, or characterize the transfected cells. Further methods can be found in PCT/US14/13048, the entirety of which is incorporated herein by reference. 
     The invention provides a computer system that is configured to implement the methods of the disclosure. The system can include a computer server (“server”) that is programmed to implement the methods described herein.  FIG. 6  depicts a system  600  adapted to enable a user to detect, analyze, and process images of cells and sequence cells. The system  600  includes a central computer server  601  that is programmed to implement exemplary methods described herein. The server  601  includes a central processing unit (CPU, also “processor”)  605  which can be a single core processor, a multi core processor, or plurality of processors for parallel processing. The server  601  also includes memory  610  (e.g. random access memory, read-only memory, flash memory); electronic storage unit  615  (e.g. hard disk); communications interface  620  (e.g. network adaptor) for communicating with one or more other systems; and peripheral devices  625  which may include cache, other memory, data storage, and/or electronic display adaptors. The memory  610 , storage unit  615 , interface  620 , and peripheral devices  625  are in communication with the processor  605  through a communications bus (solid lines), such as a motherboard. The storage unit  615  can be a data storage unit for storing data. The server  601  is operatively coupled to a computer network (“network”)  630  with the aid of the communications interface  620 . The network  630  can be the Internet, an intranet and/or an extranet, an intranet and/or extranet that is in communication with the Internet, a telecommunication or data network. The network  630  in some cases, with the aid of the server  601 , can implement a peer-to-peer network, which may enable devices coupled to the server  601  to behave as a client or a server. The microscope and micromanipulator can be peripheral devices  625  or remote computer systems  640 . 
     The storage unit  615  can store files, such as individual images, time lapse images, data about individual cells, cell colonies, or any aspect of data associated with the invention. The data storage unit  615  may be coupled with data relating to locations of cells in a virtual grid. 
     The server can communicate with one or more remote computer systems through the network  630 . The one or more remote computer systems may be, for example, personal computers, laptops, tablets, telephones, Smart phones, or personal digital assistants. 
     In some situations the system  600  includes a single server  601 . In other situations, the system includes multiple servers in communication with one another through an intranet, extranet and/or the Internet. 
     The server  601  can be adapted to store cell profile information, such as, for example, cell size, morphology, shape, migratory ability, proliferative capacity, kinetic properties, and/or other information of potential relevance. Such information can be stored on the storage unit  615  or the server  601  and such data can be transmitted through a network. 
     Methods as described herein can be implemented by way of machine (e.g., computer processor) computer readable medium (or software) stored on an electronic storage location of the server  601 , such as, for example, on the memory  610 , or electronic storage unit  615 . During use, the code can be executed by the processor  605 . In some cases, the code can be retrieved from the storage unit  615  and stored on the memory  610  for ready access by the processor  605 . In some situations, the electronic storage unit  615  can be precluded, and machine-executable instructions are stored on memory  610 . Alternatively, the code can be executed on a second computer system  640 . 
     Aspects of the systems and methods provided herein, such as the server  601 , can be embodied in programming. Various aspects of the technology may be thought of as “products” or “articles of manufacture” typically in the form of machine (or processor) executable code and/or associated data that is carried on or embodied in a type of machine readable medium (e.g., computer readable medium). Machine-executable code can be stored on an electronic storage unit, such memory (e.g., read-only memory, random-access memory, flash memory) or a hard disk. “Storage” type media can include any or all of the tangible memory of the computers, processors or the like, or associated modules thereof, such as various semiconductor memories, tape drives, disk drives and the like, which may provide non-transitory storage at any time for the software programming. All or portions of the software may at times be communicated through the Internet or various other telecommunication networks. Such communications, for example, may enable loading of the software from one computer or processor into another, for example, from a management server or host computer into the computer platform of an application server. Thus, another type of media that may bear the software elements includes optical, electrical, and electromagnetic waves, such as used across physical interfaces between local devices, through wired and optical landline networks and over various air-links. The physical elements that carry such waves, such as wired or wireless likes, optical links, or the like, also may be considered as media bearing the software. As used herein, unless restricted to non-transitory, tangible “storage” media, terms such as computer or machine “readable medium” refer to any medium that participates in providing instructions to a processor for execution. 
     Hence, a machine readable medium, such as computer-executable code, may take many forms, including but not limited to, tangible storage medium, a carrier wave medium, or physical transmission medium. Non-volatile storage media can include, for example, optical or magnetic disks, such as any of the storage devices in any computer(s) or the like, such may be used to implement the system. Tangible transmission media can include: coaxial cables, copper wires, and fiber optics (including the wires that comprise a bus within a computer system). Carrier-wave transmission media may take the form of electric or electromagnetic signals, or acoustic or light waves such as those generated during radio frequency (RF) and infrared (IR) data communications. Common forms of computer-readable media therefore include, for example: a floppy disk, a flexible disk, hard disk, magnetic tape, any other magnetic medium, a CD-ROM, DVD, DVD-ROM, any other optical medium, punch cards, paper tame, any other physical storage medium with patterns of holes, a RAM, a ROM, a PROM and EPROM, a FLASH-EPROM, any other memory chip or cartridge, a carrier wave transporting data or instructions, cables, or links transporting such carrier wave, or any other medium from which a computer may read programming code and/or data. Many of these forms of computer readable media may be involved in carrying one or more sequences of one or more instructions to a processor for execution. 
     EXAMPLES 
     Example 1: Transfection Efficiency Using a Method Disclosed Herein 
       FIG. 2  depicts an experimental procedure for comparison of electroporation versus a method of the invention, which includes hypoxic and high pressure conditions. The cells were cultured in either a standard CO 2  incubator or under hypoxic and positive pressure conditions. The cells were further cultured in media and a substrate that contained FBS or serum-free media and a serum-free substrate. 
       FIG. 3  depicts the results of the transfection of DU145 (human prostate cancer) cells. 5×10̂6 cells/mL were transfected with 0.5 μg GFP at 1260 V for 40 ms with 2 pulses. After transfection, the cells were evenly split across the tested conditions and cultured for 48 hours prior to imaging and assessment of transfection efficiency.  FIG. 3  shows that transfection of the cells under hypoxic and high pressure conditions, along with culturing in serum-free media allowed for the greatest transfection efficiency as indicated by the brightest GFP staining in the bottom-right panel. 
       FIG. 4  depicts the results of the transfection of human dermal fibroblast cells. 5×10̂6 cells/mL were transfected with 0.5 μg GFP at 1000 V for 30 ms with 1 pulse. After transfection, the cells were evenly split across the tested conditions and cultured for 48 hours prior to imaging and assessment of transfection efficiency.  FIG. 4  shows that transfection of the cells under hypoxic and high pressure conditions, along with culturing in serum-free media allowed for the greatest transfection efficiency as indicated by the brightest GFP staining in the bottom-right panel. 
       FIG. 5  depicts the results of the transfection of healthy donor peripheral blood mononuclear cells (PBMCs). 2×10̂7 cells/mL were transfected with 1 μg GFP at 1500 V for 10 ms with 1 pulse. After transfection, the cells were evenly split across the tested conditions and cultured for 48 hours prior to imaging and assessment of transfection efficiency.  FIG. 5  shows that transfection of the cells under hypoxic and high pressure conditions, along with culturing in serum-free media allowed for the greatest transfection efficiency as indicated by the brightest GFP staining in the bottom panels. 
     TABLE 1 below provides a quantitative analysis of the change in fold expression of the GFP plasmid using different methods. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Fold Expression change of GFP 
               
            
           
           
               
               
               
               
               
            
               
                 Transfection Condition 
                 DU145 
                 PC3 
                 Panc10 
                 Dermal fibroblasts 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Standard, FBS 
                 1 
                 1 
                 1 
                 1 
               
               
                 Standard, Serum-free 
                 5.56 
                 1.88 
                 0.574 
                 3.81 
               
               
                 media and substrate 
               
               
                 1% O 2  2 PSI, FBS 
                 2.55 
                 5.033 
                 15.731 
                 5.018 
               
               
                 1% O 2  2 PSI, Serum-free 
                 14.2 
                 4.234 
                 36.533 
                 6.642 
               
               
                 media and substrate 
               
               
                   
               
            
           
         
       
     
     Example 2: Transfection of Human Dermal Fibroblasts 
       FIG. 18  depicts the transfection of human dermal fibroblasts under 21% O 2  and 0 PSI, 5% O 2  and 0, 2, or 5 PSI, and 1% O 2 , and 0, 2, or 5 PSI. The cells were transfected with a GFP plasmid and imaged 48 hours post-transfection. The experiments were repeated in triplicate.  FIG. 18  shows that transfection efficiency increased at lower oxygen and higher pressure conditions as indicated by brighter GFP expression. 
       FIG. 19  provides a quantitative analysis of GFP transfection of human dermal fibroblasts grown under various hypoxic and high pressure conditions in  FIG. 18 . The results indicate that cells grown under the hypoxic and positive pressure conditions provided higher GFP transfection efficiency than cells grown under 21% O 2  and 0 PSI. 
       FIG. 53  shows the transfection of human dermal fibroblasts using electroporation of a GFP plasmid. The cells were cultured under several conditions of low oxygen and high pressure. The results indicated that 1% oxygen and 2 PSI pressure provided the greatest proportion of transfected cells (52.8% GFP+ cells versus 9.4% GFP+ under standard culture conditions). 
       FIG. 54  shows the transfection of PBMCs using electroporation of a GFP plasmid. The cells were cultured under several conditions of low oxygen and high pressure. The results indicated that 1% oxygen and 5 PSI pressure provided the greatest proportion of transfected cells (7.8% GFP+ cells versus 3.7% GFP+ under standard culture conditions). 
       FIG. 55  shows the transfection of activated CD8+ T-cells using electroporation of a GFP plasmid. The cells were cultured under several conditions of low oxygen and high pressure. The results indicated that 1% oxygen and 5 PSI pressure provided the greatest proportion of transfected cells (55.6% GFP+ cells versus 3.7% GFP+ under standard culture conditions). 
       FIG. 56  shows the post-transfection effects of CD8+ T-cells using low oxygen and high pressure conditions. The results indicated that lower oxygen (10% O 2 ) and positive pressure (5 PSI) promotes cell proliferation post-transfection (1.1×10 5  cells under higher oxygen and positive pressure versus 3×10 4  cells under standard conditions 2 days after transfection). 
     Example 3: Transfection of Immune Cells 
       FIG. 20  depicts a sample workflow for transfection of immune cells using a method disclosed herein.  FIG. 20  shows that after a sample is obtained from a donor, the sample can be enriched for CD8+ cells. A DNA plasmid is transfected using electroporation. The transfected cells are then subjected to decreasing oxygen levels and either 0 PSI or high pressure conditions. Then, the percent GFP-positive cells, percent viable cells, and relative expression of GFP in the cells are assessed. 
       FIG. 21  shows the transfection of peripheral blood mononuclear cells (PBMC) with a GFP plasmid using electroporation of cells at passage zero. The 2-5 PSI pulsed pressure condition was performed at a frequency of 30 minute pulses. The experiments were repeated four times.  FIG. 22  depicts a quantification of the results from  FIG. 21  indicating that there was an almost 2.5-fold increase in GFP-positive cells using the hypoxic and positive pressure conditions compared to standard incubator conditions. 
       FIG. 23  shows a comparison between the transfection of CD8+ cells enriched from PBMC and PBMC with a GFP plasmid using electroporation of cells at passage zero. The experiment was performed in triplicate.  FIG. 24  depicts a quantification of the results from  FIG. 23  indicating that enriched CD8+ cells have higher transfection efficiency than the PBMC. “ST” in  FIG. 24  denotes standard culture conditions. 
       FIG. 25  shows that the GFP-transfected CD8+ cells cultured under hypoxic and positive pressure conditions developed more multicellular clusters than did cells grown at standard incubator conditions. In each panel, the first number indicates the oxygen level; the second number represents the pressure level in PSI. The top left panel is a control without transfection.  FIG. 26  shows the percent GFP in the multicellular clusters in cells grown under hypoxic and positive pressure conditions compared to cells grown under standard incubator conditions.  FIG. 27  is a quantification of the results of  FIG. 26 . “ST” in  FIG. 27  denotes standard culture conditions. 
       FIG. 47  shows that hypoxic and positive pressure conditions can lead to greater enrichment of CD8+ cells from fresh blood samples than culture under standard incubator conditions. For example,  FIG. 47  shows that 8.3 million CD8+ cells were obtained after one week under hypoxic and positive pressure conditions versus 3 million CD8+ cells obtained under standard conditions. An expanded culture time up to 11 days is shown in  FIG. 48  indicating that the culture under hypoxic and positive pressure conditions generates more CD8+ cells from whole blood than culture under standard conditions. 
     Example 4: Immune Cell Genome Editing 
     A method disclosed herein can be used to introduce the CRISPR/Cas9 system into immune cells, for example, CD8+ T-cells, as shown in  FIGS. 28-30 . PBMCs were freshly isolated from healthy donors, and post-cell counting PBMCs were enriched for CD8+ cells. The CD8+ cells were cultured at 2 million cells/mL in IL-2 containing media in a standard incubator, or with PBMC media under low oxygen and positive pressure conditions. Once the cells demonstrated doubling times of 36-48 hours, the cells were transfected at 20 million cells/ml with 1 μg/μl CTLA4 CRISPR Knockout and a GFP HDR DNA plasmid. The cells were expanded under low oxygen and positive pressure conditions or under conventional culture conditions. Images were taken 1, 3, and 5 days post-transfection prior to cell splitting and counting. Quantification was performed through cell counting across entire wells and assessed as GFP+ multicellular clusters/total multicellular clusters at 5 days post-transfection as well as total cell count at 5 days post-transfection. 
       FIG. 28  shows that when a CRISPR/Cas9 system was used to knockout CTLA4, and knock-in GFP using homology-directed repair, the transfection efficiency of the CRISPR/Cas9 system was higher in the cells grown under hypoxic and high pressure conditions than in standard incubator conditions. This is indicated by the bright GFP signal seen in the top panels of  FIG. 28 . The results also indicate that the GFP expression persisted through subsequent expansion of the CD8+ cells for at least five days. The experiment was repeated four times, and the cells were transfected at passage 1+ while in the exponential growth phase. 
       FIGS. 29 and 30  provide a quantification of the results of from  FIG. 28 .  FIG. 29  shows that the cells grown under hypoxic and positive pressure conditions developed a higher percentage of GFP-positive multicellular clusters than the cells grown at standard culture conditions.  FIG. 30  shows that the proliferation of the CD8+ cells grown under hypoxic and positive pressure conditions was enriched over the cells grown under standard incubator conditions. The error bars represent standard deviation. 
       FIG. 31  depicts a limited dilution assay workflow to assess GFP-positive colonies using the CRISPR/Cas9 system. A sample is taken from a donor and then subjected to enrichment for CD8+ cells. Then, the cells are split to be cultured under either positive pressure and low oxygen conditions or under standard incubator conditions. The cells are then transfected using electroporation using a guide RNA and a homology-directed repair plasmid to effect the genomic GFP insertion. The cells are then expanded under either positive pressure and low oxygen conditions or under standard incubator conditions. After one week, the GFP-positive cells are assessed. 
       FIG. 32  shows that less input of T-cells still allowed for successful genome editing of the CD8-positive T-cells as indicated by the GFP signal visible in the cells even at the lowest concentration of 5000 cells. TABLE 2 below provides a quantification of the results of the  FIG. 32 . The results indicate that a use of hypoxic and positive pressure conditions to culture cells provided 45-times increased transfection efficiency over standard culture conditions. The p-value of the experiment was 1.68×10 −18 . 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 2 
               
             
            
               
                   
                   
               
               
                   
                 GFP+ Cell 
                   
               
            
           
           
               
               
               
            
               
                   
                   
                 Low Oxygen and Positive 
               
               
                 Cell Count 
                 Standard 
                 Pressure 
               
               
                   
               
            
           
           
               
               
               
            
               
                 5000 
                 0/12 
                  1/12 
               
               
                 10000 
                 0/12 
                 12/12 
               
               
                 50000 
                 1/12 
                 12/12 
               
               
                 100000 
                 4/12 
                 12/12 
               
               
                   
               
            
           
         
       
     
       FIGS. 59-60  provide molecular confirmation of the genome editing experiments performed above.  FIG. 59  provides data relating to the PD1 knockout, and  FIG. 60  provides data relating to the CTLA4 knockout. Both figures show that the highlighted bands in the DNA gels (upper panels) were extracted and used as a template for sequencing. For  FIG. 59 , genome editing was confirmed for Donors 76, 78, 82, and MOLT4. The PD1-pos-HDR was a positive control from previous MOLT4 knockout cells. For  FIG. 60 , genome editing was confirmed for Donors 74, 75, and 76. 
     Example 5: Reprogramming of Cells Using a Method of the Invention 
       FIG. 7  depicts a workflow of a stem cell reprogramming experiment using a method of the invention. Prior to initiation of the reprogramming process, human fibroblasts were plated in fibroblast medium until the cells reached a desired confluency. The cells were cultured either under standard conditions or under hypoxic and positive pressure conditions. The fibroblasts were then transfected with a RNA vector encoding Oct4, KLF-4, SOX2, GLIS1, and c-MYC, and a puromycin resistance gene. After 5 days of puromycin selection post-transfection, the cells were cultured in reprogramming media for the remainder of the reprogramming induction phase until the induced pluripotent stem cell (iPSC) colonies emerged. Recombinant B18R Protein was also added during the first 2 weeks after transfection to inhibit the interferon response and increase cell viability. After about 20 days, the iPSC colonies were isolated and propagated in maintenance media. The maintenance media was a complete, serum-free media designed for the feeder-free maintenance and expansion of stem cells. The maintenance media contained recombinant human basic fibroblast growth factor and recombinant human transforming growth factor β. The reprogramming media was a complete, xeno-free defined reprogramming media designed for generating iPSCs under feeder-free conditions. 
     The colonies formed from standard or hypoxic and high pressure conditions were assessed for various markers.  FIG. 8  shows that by day 19, the average fold increase in colony number was higher in cells grown under hypoxic and either standard or high pressure conditions as compared to standard conditions (18% oxygen and 0 PSI). For example, cells grown under 5% oxygen and 0 PSI had about a 13-fold increase in colony number as compared to cells grown in 18% oxygen and 0 PSI, which increase was statistically significant. Further, cells grown under 5% oxygen and 2 PSI had about a 10-fold increase in colony number as compared to cells grown in 18% oxygen and 0 PSI, which increase was statistically significant. 
       FIG. 9  depicts the frequency distribution of colony area between cells cultured in 18% oxygen; 0 PSI, 5% oxygen; 0 PSI, and 5% oxygen; 2 PSI conditions. The graph illustrates that the colony area (measured in μm 2 ), was greater in cells grown under hypoxic and either standard or positive pressure conditions. 
       FIG. 10  shows microscopy images of the morphology of cells grown at 18% oxygen; 0 PSI, 5% oxygen; 0 PSI, and 5% oxygen; 2 PSI conditions. The images show that cells grown under hypoxic and either standard or high pressure conditions had a greater cell area than those cells grown under standard conditions. 
       FIG. 11  shows the reprogramming kinetics of cells grown under 18% oxygen; 0 PSI (standard), 5% oxygen; 0 PSI, and 5% oxygen; 2 PSI conditions. The graph shows that cells grown under hypoxic and either standard or positive pressure conditions had a higher rate of reprogramming, as indicated by the increase in stem cell colony count per days post-transfection, as compared to cells grown under standard conditions. 
       FIG. 12  shows the effect of hypoxia and positive pressure conditions on pre-cardiomyocyte differentiation and morphology. H9c2 pre-cardiomyocytes were cultured under 20% oxygen; 0 PSI (standard conditions), 5% oxygen; 2 PSI, and 1% oxygen; 5 PSI. The cells were then stained with DAPI to identify the nuclei of the cells, F-actin to visualize the individual cells, and cardiac troponin, a marker of cardiomyocytes. The results indicated that with decreasing oxygen levels and increasing pressure levels, the cells expressed increasing levels of cardiac troponin as shown by the more intense staining in the cytoplasm of the cells. 
       FIG. 13  shows staining of fibroblasts with DAPI to identify the nuclei of the cells and Sox2, a stem cell marker. After removal of pluripotency supporting mouse embryonic fibroblasts (MEFs), the cells grown under standard conditions (20% oxygen and 0 PSI) differentiated, while the cells grown under 1% oxygen and 2 PSI conditions maintained their dedifferentiated state. The differentiation was indicated by the Sox2 staining and the morphology of the cells after culture in the varying oxygen and pressure conditions. The cells were assessed for other stem cells markers by qPCR as shown in  FIG. 14 . 
       FIG. 14  shows that the cells grown under 1% oxygen and 2 PSI had higher levels of Nanog, Oct4, and Sox2 as compared to cells grown under standard conditions. 
       FIG. 15  shows staining of fibroblasts 23 days post-transfection of the reprogramming RNA vector as described above under 20% oxygen; 0 PSI (standard conditions); 5% oxygen; 0 PSI, and 5% oxygen; 2 PSI. The cells were stained with DAPI, Sox2, and SSEA4, the latter two of which are stem cells markers. Cells of the same size from each condition were analyzed for expression of SSEA4, a human embryonic stem cell marker. The results indicated that the cells grown under hypoxic and either standard or high pressure conditions showed greater staining for SSEA4, as indicated by the more intense staining around the periphery of the cells in  FIG. 15 .  FIG. 16  shows the average colony area over the experimental period for the aforementioned conditions. 
       FIG. 49  shows induction of neural precursor markers, PAX6 and NESTIN, in iPSCs after two weeks in culture under 5% O 2  and 2 PSI in stem cell maintenance media. 
       FIG. 33  shows that a combination of low oxygen and positive pressure enhances ectoderm commitment in defined medium, while causing changes in colony morphology to more mesoderm-like morphology. The directed differentiation of iPSCs to all three germ layers was performed using defined medium under the indicated cell culture conditions. Each experiment was performed in triplicate using independent iPSC reprogrammed cells lines at passage 5. The results indicate that the brachyury (mesoderm indicator) was more prominent along the edges of the cells under culture at hypoxic and high pressure conditions. FOXA2 (endoderm indicator) was more prominent in cells under culture at hypoxic and high pressure conditions. Finally, PAX6 (ectoderm indicator) was more highly expressed in the cells under grown under low oxygen and high pressure conditions. 
       FIG. 34  shows that induction of PAX6 in iPSCs was accompanied by a loss of E-cadherin (indicated by CDH1 staining) under conditions of low oxygen and positive pressure (A; left panel is PAX6 staining; right panel is CDH1 staining). Additionally, SOX2, SSEA4, and NANOG staining decreased at low oxygen and positive pressure conditions, while OCT4 remained fairly constant throughout the three experimental conditions (B; left panel is SOX2 staining, right panel is SSEA4 staining. C; left panel is OCT4 staining, right panel is NANOG staining). 
       FIG. 17  shows the gene expression profiles as a function of oxygen concentration and pressure as compared to standard incubation conditions. The results indicated that oxygen concentration and pressure had an effect on the gene expression profile of several iPSC marker genes of interest.  FIG. 45  shows the effect that various oxygen and pressure conditions had on the gene expression of immunotherapeutic targets in donor PBMCs. The cell culture conditions were created to mimic the vasculature and tumor microenvironments. The sample size for each condition was 12. The results of  FIG. 45  are quantified (on a logarithmic scale) in TABLE 3 below. 
     
       
         
           
               
               
               
               
               
               
               
             
               
                   
                 TABLE 3 
               
               
                   
                   
               
               
                   
                 Donor 1 
                 Donor 2 
                 Donor 3 
                 Donor 4 
                 Donor 5 
                 Donor 6 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 TNFRSF4 
                 6.619909 
                 6.866825 
                 6.948752 
                 6.77406 
                 7.199577 
                 7.044961 
               
               
                 TNFRSF9 
                 7.11055 
                 7.182824 
                 7.046122 
                 7.770995 
                 7.907799 
                 8.281298 
               
               
                 TNFSF4 
                 6.959052 
                 7.238442 
                 6.69186 
                 7.131686 
                 7.270319 
                 7.912743 
               
               
                 IL10 
                 7.571088 
                 7.472796 
                 8.289168 
                 8.460592 
                 10.14985 
                 9.663655 
               
               
                 IL20 
                 3.923793 
                 3.7464 
                 3.066 
                 3.650412 
                 4.757204 
                 3.658009 
               
               
                 GNLY 
                 11.58694 
                 11.58746 
                 11.92236 
                 12.00283 
                 12.14272 
                 12.32933 
               
               
                 CD8A 
                 10.58403 
                 10.74872 
                 11.73939 
                 11.03479 
                 11.5482 
                 11.26316 
               
               
                 IL1B 
                 12.26734 
                 12.36899 
                 13.85733 
                 13.13851 
                 17.2512 
                 16.41079 
               
               
                 CTLA4 
                 7.218212 
                 7.548015 
                 7.96451 
                 8.191502 
                 8.123371 
                 8.438327 
               
               
                 ICOS 
                 8.459099 
                 8.514474 
                 9.260944 
                 9.310994 
                 9.568987 
                 9.989088 
               
               
                 PDCD1 
                 5.298568 
                 5.887669 
                 5.870134 
                 5.689587 
                 6.187069 
                 6.070108 
               
               
                 CX3CR1 
                 6.848331 
                 6.521741 
                 6.770097 
                 6.755467 
                 7.244198 
                 7.098837 
               
               
                 BTLA 
                 6.550678 
                 6.491376 
                 7.731419 
                 7.463986 
                 8.060028 
                 7.759103 
               
               
                 IL12A 
                 5.238696 
                 4.780255 
                 5.055161 
                 4.893 
                 4.910049 
                 5.020782 
               
               
                 CXCL9 
                 4.88878 
                 4.39225 
                 4.078183 
                 6.442779 
                 5.653539 
                 4.950788 
               
               
                 CXCL10 
                 6.477897 
                 6.717857 
                 5.181365 
                 7.381816 
                 4.957034 
                 4.986273 
               
               
                 CXCL13 
                 8.705631 
                 8.945058 
                 8.734548 
                 9.69251 
                 9.49176 
                 10.32852 
               
               
                 IL13 
                 3.67695 
                 4.39225 
                 4.078183 
                 4.453252 
                 3.883496 
                 4.668556 
               
               
                 IL4 
                 4.262466 
                 4.885367 
                 4.356257 
                 5.157029 
                 3.736401 
                 4.668556 
               
               
                 HAVCR2 
                 9.170135 
                 9.146919 
                 9.582496 
                 9.055441 
                 8.495221 
                 8.383747 
               
               
                 MICA 
                 9.5119 
                 9.485452 
                 8.457327 
                 8.581001 
                 8.713503 
                 8.310856 
               
               
                 VEGFA 
                 10.82009 
                 10.66857 
                 11.57516 
                 10.85657 
                 12.58646 
                 12.20046 
               
               
                 IL17A 
                 4.394877 
                 4.019712 
                 3.658276 
                 4.555729 
                 5.5696 
                 4.950788 
               
               
                 IL6 
                 10.67563 
                 10.36244 
                 9.83626 
                 11.30245 
                 13.4968 
                 12.44336 
               
               
                 FOXP3 
                 7.649995 
                 7.533283 
                 8.282547 
                 8.390411 
                 8.306643 
                 8.195861 
               
               
                 CD40LG 
                 6.142305 
                 6.637095 
                 6.475081 
                 7.027289 
                 6.392924 
                 7.242464 
               
               
                 IDO1 
                 10.16081 
                 10.41552 
                 9.648315 
                 11.00966 
                 9.463818 
                 10.17725 
               
               
                 IL7 
                 5.298568 
                 5.509989 
                 5.591429 
                 5.876287 
                 6.829225 
                 6.680652 
               
               
                 CD274 
                 9.902727 
                 9.830315 
                 8.97929 
                 9.246269 
                 10.94272 
                 10.59646 
               
               
                 PDCD1LG2 
                 9.004413 
                 8.817339 
                 6.071134 
                 7.61544 
                 6.963616 
                 6.700756 
               
               
                 IL33 
                 7.31835 
                 7.105141 
                 3.066 
                 10.27267 
                 3.883496 
                 3.066 
               
               
                 IL15 
                 7.450272 
                 7.687581 
                 7.290701 
                 7.714615 
                 7.036545 
                 7.434076 
               
               
                 PRF1 
                 9.608044 
                 9.799925 
                 10.05587 
                 10.23814 
                 9.88916 
                 10.17636 
               
               
                 CD27 
                 7.499824 
                 7.739823 
                 8.341071 
                 7.842887 
                 7.983301 
                 8.152978 
               
               
                 LAG3 
                 7.703818 
                 7.605478 
                 7.701923 
                 8.135379 
                 8.251017 
                 8.432363 
               
               
                 CD4 
                 9.963468 
                 10.03466 
                 10.39339 
                 10.6011 
                 10.25358 
                 10.45002 
               
               
                 IFNG 
                 3.066 
                 4.019712 
                 4.356257 
                 4.339943 
                 4.701601 
                 5.020782 
               
               
                 CXCL1 
                 13.46389 
                 13.51905 
                 14.50338 
                 14.0498 
                 15.36751 
                 14.96332 
               
               
                 CCL7 
                 10.178 
                 10.03205 
                 11.89334 
                 9.941749 
                 6.974265 
                 7.373004 
               
               
                 CCL5 
                 11.94975 
                 12.08067 
                 12.31532 
                 12.19939 
                 11.9531 
                 12.05798 
               
               
                 CD40 
                 8.757388 
                 8.690513 
                 9.051137 
                 8.895786 
                 8.983975 
                 8.414324 
               
               
                 CD70 
                 5.700041 
                 5.446335 
                 5.546533 
                 6.159697 
                 5.988991 
                 5.736881 
               
               
                 ICAM1 
                 10.56529 
                 10.50489 
                 9.923909 
                 10.34862 
                 10.86652 
                 10.23831 
               
               
                 TGFB1 
                 11.26375 
                 11.17533 
                 11.36419 
                 11.25554 
                 11.26814 
                 11.0061 
               
               
                 CD33 
                 7.582629 
                 7.291983 
                 9.093345 
                 7.724167 
                 7.278922 
                 6.414203 
               
               
                   
               
            
           
         
       
     
     TABLE 4 below shows the effect that high atmospheric pressure had on iPSCs grown under hypoxic conditions as assessed by digital PCR. The results indicate that that was a change in gene expression of various neuronal, bone, and cardiomyocyte factors. TABLE 4 shows relative gene expression changes with 1 being no change, and values above 1 indicating greater expression, and values below 1 indicating lower expression. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 4 
               
               
                   
                   
               
               
                   
                 iPSC 
                 iPSC 
               
               
                   
                 5% O 2  + 0 PSI 
                 5% O 2  + 2 PSI 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 PAX6 
                 13.167 
                 116.833 
               
               
                   
                 FABP7 
                 3.083 
                 7.488 
               
               
                   
                 LEFTY2 
                 3.047 
                 1.526 
               
               
                   
                 COL2A1 
                 2.997 
                 21.564 
               
               
                   
                 FOXA2 
                 2.761 
                 1.674 
               
               
                   
                 BMP2 
                 2.682 
                 2.071 
               
               
                   
                 OTX2 
                 2.179 
                 2.513 
               
               
                   
                 NCAM1 
                 2.038 
                 3.667 
               
               
                   
                 CDH2 
                 2.025 
                 7.926 
               
               
                   
                 GATA2 
                 2.000 
                 1.000 
               
               
                   
                 FGFR1 
                 1.994 
                 1.683 
               
               
                   
                 COL9A1 
                 1.791 
                 1.764 
               
               
                   
                 ACTC1 
                 1.784 
                 7.826 
               
               
                   
                 RUNX1 
                 1.667 
                 7.667 
               
               
                   
                 COMMD3 
                 1.656 
                 4.008 
               
               
                   
                 PTEN 
                 1.574 
                 1.902 
               
               
                   
                 COL1A1 
                 1.568 
                 2.147 
               
               
                   
                 NES 
                 1.535 
                 3.579 
               
               
                   
                 ALDH2 
                 1.444 
                 2.235 
               
               
                   
                 SOX2 
                 1.438 
                 1.472 
               
               
                   
                 CDC42 
                 1.387 
                 1.239 
               
               
                   
                 EP300 
                 1.382 
                 1.412 
               
               
                   
                 FGF2 
                 1.343 
                 0.948 
               
               
                   
                 REST 
                 1.323 
                 1.651 
               
               
                   
                 HSPA9 
                 1.276 
                 1.060 
               
               
                   
                 GPI 
                 1.274 
                 1.253 
               
               
                   
                 GABRB3 
                 1.264 
                 0.821 
               
               
                   
                 CCNA2 
                 1.259 
                 1.173 
               
               
                   
                 MYBL2 
                 1.258 
                 1.165 
               
               
                   
                 CDK1 
                 1.229 
                 1.233 
               
               
                   
                 LEFTY1 
                 1.225 
                 0.360 
               
               
                   
                 TCF3 
                 1.218 
                 1.439 
               
               
                   
                 ALPL 
                 1.209 
                 1.119 
               
               
                   
                 DPPA3 
                 1.208 
                 0.531 
               
               
                   
                 SFRP2 
                 1.197 
                 1.921 
               
               
                   
                 GRB7 
                 1.189 
                 0.533 
               
               
                   
                 PSMB4 
                 1.167 
                 0.997 
               
               
                   
                 NAT1 
                 1.151 
                 0.792 
               
               
                   
                 APC 
                 1.145 
                 1.361 
               
               
                   
                 GDF3 
                 1.138 
                 0.443 
               
               
                   
                 REEP5 
                 1.133 
                 0.996 
               
               
                   
                 LIN28A 
                 1.125 
                 1.065 
               
               
                   
                 BRIX1 
                 1.111 
                 0.957 
               
               
                   
                 PSMB2 
                 1.104 
                 1.016 
               
               
                   
                 NUMB 
                 1.090 
                 1.090 
               
               
                   
                 HPRT1 
                 1.089 
                 0.774 
               
               
                   
                 AFP 
                 1.082 
                 1.135 
               
               
                   
                 CCNE1 
                 1.073 
                 0.977 
               
               
                   
                 PODXL 
                 1.068 
                 0.737 
               
               
                   
                 GJA1 
                 1.067 
                 1.177 
               
               
                   
                 PARD6A 
                 1.060 
                 0.766 
               
               
                   
                 KAT7 
                 1.052 
                 1.037 
               
               
                   
                 KAT8 
                 1.019 
                 0.843 
               
               
                   
                 POU5F1 
                 0.982 
                 0.548 
               
               
                   
                 RAB7A 
                 0.973 
                 1.062 
               
               
                   
                 CDH1 
                 0.954 
                 0.365 
               
               
                   
                 KAT2A 
                 0.946 
                 0.996 
               
               
                   
                 DNMT3B 
                 0.935 
                 0.589 
               
               
                   
                 CD9 
                 0.894 
                 0.398 
               
               
                   
                 FOXD3 
                 0.880 
                 0.343 
               
               
                   
                 DPPA2 
                 0.876 
                 0.425 
               
               
                   
                 MYCN 
                 0.867 
                 1.018 
               
               
                   
                 KLF4 
                 0.806 
                 1.032 
               
               
                   
                 MYC 
                 0.759 
                 0.822 
               
               
                   
                 TDGF1 
                 0.757 
                 0.291 
               
               
                   
                 TBX3 
                 0.750 
                 1.250 
               
               
                   
                 HDAC2 
                 0.739 
                 0.773 
               
               
                   
                 ALDH1A1 
                 0.708 
                 1.542 
               
               
                   
                 RUNX2 
                 0.684 
                 0.947 
               
               
                   
                 EMX2 
                 0.667 
                 44.000 
               
               
                   
                 ZFP42 
                 0.606 
                 0.464 
               
               
                   
                 NODAL 
                 0.583 
                 0.313 
               
               
                   
                 GAL 
                 0.485 
                 0.175 
               
               
                   
                 NANOG 
                 0.485 
                 0.182 
               
               
                   
                 NR5A2 
                 0.218 
                 0.113 
               
               
                   
                 FGF5 
                   
                 1.500 
               
               
                   
                   
               
            
           
         
       
     
     Example 6: Change in Gene Expression in Cancer Cells 
       FIG. 35  show that different combinations of tumor (disease) extracellular matrix (ECM), low oxygen, and high pressure can alter the gene expression of EGFR and other metabolic regulators in DU145 (prostate cancer) and Panc10 (pancreatic cell lines). The markers measured included EGFR, ErbB2, LDHA, SLC1A5, and TRX1. The results indicate that, generally, gene expression increased with low oxygen, high pressure, and tumor ECM conditions. However, for ErbB2 in DU145 cells, the ErbB2 expression decreased compared to standard incubator conditions when the cells were cultured under hypoxic and high pressure conditions. 
       FIG. 36  shows that PDL1 expression increased in ARV7-positive, 22RV1 prostate cancer cells during low oxygen and positive pressure culturing conditions. The top panel of  FIG. 37  provides a western blot showing increased PDL1 protein expression under various conditions of high pressure and hypoxia in both DU145 and 22Rv1 prostate cancer cells. The bottom panel of  FIG. 37  provides a quantification of the western blot results normalized to actin. The results indicated that the change in PDL1 expression was more pronounced in the 22Rv1 prostate cancer cells, rather than the DU145 prostate cancer cells. 
     TABLES 5-10 below show the effect of varying oxygen and pressure on the gene expression profiles of various cancer targets when compared to traditional culturing approaches. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 LnCAP (2 PSI) 
               
            
           
           
               
               
               
               
            
               
                   
                 1% O 2   
                 10% O 2   
                 20% O 2   
               
               
                   
                   
               
               
                   
                 VEGFA (higher) 
                 EGFR (higher) 
                 PRPF40A (lower) 
               
               
                   
                 GPI (higher) 
                 GPI (higher) 
                 SSSCA1 (lower) 
               
               
                   
                 HK2 (higher) 
                 HSP90B1 (lower) 
                 VEGFA (higher) 
               
               
                   
                 HSP90B1 (lower) 
                 VEGFA (higher) 
                 EGFR (higher) 
               
               
                   
                 EPAS1 (lower) 
                 GPX1 (lower) 
                 NFκB1 (lower) 
               
               
                   
                 EGFR (higher) 
                 SPG7 (lower) 
                 PARP2 (lower) 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 LnCAP (5 PSI) 
               
            
           
           
               
               
               
               
            
               
                   
                 1% O 2   
                 10% O 2   
                 20% O 2   
               
               
                   
                   
               
               
                   
                 GPI (higher) 
                 EGFR (higher) 
                 NFκB1 (lower) 
               
               
                   
                 HK2 (higher) 
                 HDAC3 (higher) 
                 HDAC3 (higher) 
               
               
                   
                 ITGAV (lower) 
                 PK3C2A (higher) 
                 LAMA3 (lower) 
               
               
                   
                 EGFR (higher) 
                 PLOD3 (higher) 
                 PARP2 (lower) 
               
               
                   
                 VEGFA (higher) 
                 GP1 (higher) 
                 HSP90B1 (lower) 
               
               
                   
                 HDAC3 (higher) 
                 CDK5 (lower) 
                 SSSCA1 (higher) 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 PC-3 (2 PSI) 
               
            
           
           
               
               
               
               
            
               
                   
                 1% O 2   
                 10% O 2   
                 20% O 2   
               
               
                   
                   
               
               
                   
                 ATF2 (higher) 
                 ITGAV (lower) 
                 SSSCA1 (lower) 
               
               
                   
                 HDAC3 (higher) 
                 ATF2 (lower) 
                 PEA15 (higher) 
               
               
                   
                 GP1 (higher) 
                 PEA15 (lower) 
                 CDK2 (higher) 
               
               
                   
                 PEA15 (lower) 
                 IGF1R (lower) 
                 ATF2 (higher) 
               
               
                   
                 HK2 (higher) 
                 PLK4 (higher) 
                 NFκB1 (lower) 
               
               
                   
                 HPRT1 (higher) 
                 RHOB (lower) 
                 ABCC1 (lower) 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 PC-3 (5 PSI) 
               
            
           
           
               
               
               
               
            
               
                   
                 1% O 2   
                 10% O 2   
                 20% O 2   
               
               
                   
                   
               
               
                   
                 GP1 (higher) 
                 PI3KC2a (lower) 
                 P992CB (higher) 
               
               
                   
                 HK2 (higher) 
                 CDC25A (lower) 
                 CDK7 (lower) 
               
               
                   
                 PARP2 (lower) 
                 PLK4 (lower) 
                 HDAC3 (lower) 
               
               
                   
                 PLK1 (lower) 
                 PRPF40A (lower) 
                 ERBB2 (higher) 
               
               
                   
                 SSSCA1 (lower) 
                 MAN2B1 (higher) 
                 PEA15 (higher) 
               
               
                   
                 AURKA (lower) 
                 HSP90B1 (higher) 
                 HK2 (lower) 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 PANC-10.05 (2 PSI) 
               
            
           
           
               
               
               
               
            
               
                   
                 1% O 2   
                 10% O 2   
                 20% O 2   
               
               
                   
                   
               
               
                   
                 GP1 (higher) 
                 GP1 (higher) 
                 CTSB (lower) 
               
               
                   
                 ITGAV (lower) 
                 SPG7 (lower) 
                 ITGAL (lower) 
               
               
                   
                 VEGFA (higher) 
                 PARP2 (lower) 
                 ITGAV (lower) 
               
               
                   
                 CDK7 (lower) 
                 CDC25A (lower) 
                 CDK2 (higher) 
               
               
                   
                 MAN2B1 (higher) 
                 MMP1 (higher) 
                 GPI (higher) 
               
               
                   
                 HRAS (lower) 
                 COL12A1 (lower) 
                 ENO1 (lower) 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 PANC-10.05 (5 PSI) 
               
            
           
           
               
               
               
               
            
               
                   
                 1% O 2   
                 10% O 2   
                 20% O 2   
               
               
                   
                   
               
               
                   
                 GP1 (higher) 
                 GP1 (higher) 
                 RHOB (lower) 
               
               
                   
                 VEGFA (higher) 
                 CDK5 (higher) 
                 CTSB (lower) 
               
               
                   
                 ADM (higher) 
                 CDC25A (lower) 
                 BIRC5 (lower) 
               
               
                   
                 DR1 (lower) 
                 PPP2CB (higher) 
                 ITGAL (lower) 
               
               
                   
                 HK2 (higher) 
                 TXN_ALT (higher) 
                 PIK3C3 (lower) 
               
               
                   
                 NAA10 (lower) 
                 TXNRD1 (higher) 
                 ITGAV (lower) 
               
               
                   
                   
               
            
           
         
       
     
       FIG. 38  shows identification of pressure and oxygen sensitive gene expression signatures in various cell lines. The results indicated that there was an enrichment of metabolic processes involved in cell survival. The results in the top panel of  FIG. 38  were corrected for any false discovery rate. The top panel of  FIG. 38  provides various gene ontology terms that were enriched under low oxygen or positive pressure conditions. The bars to the right of the 0 on the x-axis indicated upregulated genes, and the bars to the left of the 0 on the x-axis indicated downregulated genes. The bottom panel of  FIG. 38  shows that over 130 genes were shown to be co-regulated by low oxygen and positive pressure conditions across various cell lines. 
       FIGS. 57 and 58  show the effect of various experimental conditions on various cells lines.  FIG. 57  shows pressure-sensitive genes, while  FIG. 58  depicts oxygen-sensitive genes. The cells lines tested for both figures was (from left to right) PANC10, LNCaP, PC3, and 22Rv1. Within each group of cell lines, the cells were exposed to high (&gt;18%; left-most columns of cells) and low (&lt;5%; right-most columns of cells) oxygen, low pressure (0 PSI; left-most columns of cells) and positive pressure (2 PSI; right-most columns of cells). TABLES 11-16 below provide the quantitative data for the heatmaps of  FIGS. 57-58 . 
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Oxygen-Sensitive Candidates 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 O2 
                 PSI 
                 Expt 
                 CellLine 
                 FUT11 
                 PFKFB4 
                 PPFIA4 
                 ANKZF1 
                 PFKFB3 
                 MIR210HG 
                 BNIP3L 
                 LDHA 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 LNCaP_25 
                 20 
                 0 
                 A 
                 LNCaP 
                 148.8648 
                 142.0982 
                 8.458226 
                 419.528 
                 316.3376 
                 10.14987 
                 1283.959 
                 11777.23 
               
               
                 22RV1_3 
                 20 
                 0 
                 A 
                 22RV1 
                 221.0045 
                 97.77776 
                 6.697107 
                 420.5783 
                 297.3515 
                 18.7519 
                 488.8888 
                 10510.44 
               
               
                 PC3_3 
                 20 
                 0 
                 A 
                 PC3 
                 982.6681 
                 450.2195 
                 9.330973 
                 306.7557 
                 521.9513 
                 20.4115 
                 713.2363 
                 19104.58 
               
               
                 DU145_3 
                 20 
                 0 
                 A 
                 DU145 
                 374.04 
                 107.181 
                 41.56 
                 192.4884 
                 1296.016 
                 28.43579 
                 3087.47 
                 17411.45 
               
               
                 PANC10_12 
                 20 
                 0 
                 B 
                 PANC10 
                 261.2557 
                 36.13111 
                 2.779316 
                 141.7451 
                 390.4939 
                 16.6759 
                 922.733 
                 18838.21 
               
               
                 LNCaP_1 
                 20 
                 0 
                 B 
                 LNCaP 
                 157.7928 
                 136.224 
                 1.1352 
                 407.5369 
                 294.0168 
                 21.5688 
                 1683.502 
                 11943.44 
               
               
                 22RV1_12 
                 20 
                 0 
                 B 
                 22RV1 
                 222.767 
                 104.2549 
                 6.237475 
                 440.1875 
                 308.3095 
                 24.9499 
                 619.2922 
                 9452.448 
               
               
                 DU145_12 
                 20 
                 0 
                 B 
                 DU145 
                 446.3176 
                 143.8638 
                 32.85079 
                 190.308 
                 1184.894 
                 52.10815 
                 3061.92 
                 23495.11 
               
               
                 PANC10_21 
                 20 
                 0 
                 C 
                 PANC10 
                 338.7363 
                 30.03138 
                 1.766552 
                 150.1569 
                 314.0046 
                 18.10716 
                 1237.028 
                 22815.9 
               
               
                 LNCaP_10 
                 20 
                 0 
                 C 
                 LNCaP 
                 159.8962 
                 153.9 
                 8.994158 
                 376.7553 
                 293.8092 
                 17.98832 
                 1323.141 
                 11189.73 
               
               
                 22RV1_21 
                 20 
                 0 
                 C 
                 22RV1 
                 195.1418 
                 84.6398 
                 2.351106 
                 391.4591 
                 246.8661 
                 28.21327 
                 739.4227 
                 8315.861 
               
               
                 PC3_25 
                 20 
                 0 
                 C 
                 PC3 
                 670.9391 
                 423.8815 
                 8.262797 
                 356.1266 
                 355.3003 
                 13.22048 
                 796.5337 
                 17632.81 
               
               
                 DU145_21 
                 20 
                 0 
                 C 
                 DU145 
                 371.5608 
                 119.1418 
                 33.31931 
                 209.0029 
                 1529.659 
                 36.34834 
                 2347.497 
                 18875.89 
               
               
                 PANC10_8 
                 20 
                 0 
                 A 
                 PANC10 
                 271.5097 
                 21.81774 
                 4.242339 
                 199.996 
                 283.6307 
                 12.12097 
                 994.5255 
                 21212.91 
               
               
                 LNCaP_26 
                 20 
                 0 
                 A 
                 LNCaP 
                 193.4126 
                 109.4788 
                 3.649293 
                 385.0005 
                 328.4364 
                 18.24647 
                 1591.092 
                 13212.27 
               
               
                 22RV1_6 
                 20 
                 0 
                 A 
                 22RV1 
                 236.1916 
                 112.9612 
                 4.107679 
                 465.1947 
                 291.6452 
                 15.4038 
                 626.4211 
                 9411.72 
               
               
                 PC3_6 
                 20 
                 0 
                 A 
                 PC3 
                 814.0391 
                 385.4435 
                 16.82201 
                 409.5794 
                 430.7898 
                 22.67315 
                 815.5019 
                 21881.78 
               
               
                 DU145_6 
                 20 
                 0 
                 A 
                 DU145 
                 390.234 
                 120.8689 
                 34.53398 
                 188.7858 
                 1281.211 
                 35.68512 
                 3075.827 
                 18655.26 
               
               
                 PANC10_15 
                 20 
                 0 
                 B 
                 PANC10 
                 266.3327 
                 11.38174 
                 1.138174 
                 161.6207 
                 357.3867 
                 15.93444 
                 1108.582 
                 20638.51 
               
               
                 LNCaP_4 
                 20 
                 0 
                 B 
                 LNCaP 
                 218.3846 
                 168.5067 
                 4.04416 
                 413.8524 
                 376.1069 
                 22.91691 
                 1480.163 
                 12063.73 
               
               
                 22RV1_15 
                 20 
                 0 
                 B 
                 22RV1 
                 224.198 
                 107.2251 
                 3.899096 
                 416.2285 
                 296.3313 
                 16.57116 
                 611.1833 
                 9461.156 
               
               
                 PC3_15 
                 20 
                 0 
                 B 
                 PC3 
                 1154.691 
                 535.3124 
                 26.03343 
                 434.9752 
                 612.328 
                 71.04957 
                 896.5263 
                 22944.13 
               
               
                 DU145_15 
                 20 
                 0 
                 B 
                 DU145 
                 500.0377 
                 221.7909 
                 62.50471 
                 264.1328 
                 1630.163 
                 87.70823 
                 2433.651 
                 26165.28 
               
               
                 PANC10_24 
                 20 
                 0 
                 C 
                 PANC10 
                 279.1962 
                 23.19056 
                 4.547169 
                 155.9679 
                 344.6754 
                 12.27736 
                 968.0923 
                 18497.43 
               
               
                 LNCaP_13 
                 20 
                 0 
                 C 
                 LNCaP 
                 191.8114 
                 184.7509 
                 5.883786 
                 398.9207 
                 303.6034 
                 25.88866 
                 1423.876 
                 11905.25 
               
               
                 22RV1_24 
                 20 
                 0 
                 C 
                 22RV1 
                 222.8496 
                 119.8661 
                 5.064763 
                 446.5433 
                 254.0823 
                 27.8562 
                 882.9571 
                 8997.552 
               
               
                 PC3_26 
                 20 
                 0 
                 C 
                 PC3 
                 752.2415 
                 421.9328 
                 6.159603 
                 333.3885 
                 432.7121 
                 12.31921 
                 878.5134 
                 18287.86 
               
               
                 DU145_24 
                 20 
                 0 
                 C 
                 DU145 
                 315.9268 
                 100.4485 
                 42.12358 
                 241.4005 
                 1213.483 
                 42.12358 
                 1699.524 
                 15059.18 
               
               
                 PANC10_9 
                 20 
                 0 
                 A 
                 PANC10 
                 308.4363 
                 25.97358 
                 0 
                 220.7754 
                 269.4759 
                 12.98679 
                 1042.19 
                 19941.22 
               
               
                 LNCaP_27 
                 20 
                 0 
                 A 
                 LNCaP 
                 187.4626 
                 94.73918 
                 0 
                 417.2555 
                 264.0603 
                 20.15727 
                 1408.993 
                 12487.43 
               
               
                 22RV1_9 
                 20 
                 0 
                 A 
                 22RV1 
                 221.6903 
                 108.7925 
                 0 
                 472.1183 
                 279.1656 
                 12.31613 
                 665.071 
                 9027.723 
               
               
                 PC3_9 
                 20 
                 0 
                 A 
                 PC3 
                 1047.944 
                 516.1348 
                 17.91357 
                 383.4624 
                 615.779 
                 34.14775 
                 1031.15 
                 25294.52 
               
               
                 DU145_9 
                 20 
                 0 
                 A 
                 DU145 
                 525.8409 
                 352.0274 
                 81.40633 
                 323.4251 
                 1627.027 
                 82.50642 
                 3651.184 
                 22824.57 
               
               
                 PANC10_18 
                 20 
                 0 
                 B 
                 PANC10 
                 294.2244 
                 34.51794 
                 9.862269 
                 198.8891 
                 295.8681 
                 32.87423 
                 1290.313 
                 22765.4 
               
               
                 LNCaP_7 
                 20 
                 0 
                 B 
                 LNCaP 
                 208.0811 
                 123.3848 
                 4.182534 
                 367.0174 
                 324.1464 
                 13.59324 
                 1846.589 
                 13925.75 
               
               
                 22RV1_18 
                 20 
                 0 
                 B 
                 22RV1 
                 251.6665 
                 100.153 
                 3.852038 
                 376.2157 
                 290.1869 
                 19.26019 
                 735.7393 
                 8831.439 
               
               
                 PC3_18 
                 20 
                 0 
                 B 
                 PC3 
                 876.1958 
                 531.8069 
                 48.03854 
                 466.1768 
                 508.1259 
                 40.59595 
                 883.6384 
                 21119.36 
               
               
                 DU145_18 
                 20 
                 0 
                 B 
                 DU145 
                 358.0772 
                 119.3591 
                 30.744 
                 233.2927 
                 888.8633 
                 55.15835 
                 2062.561 
                 18450.92 
               
               
                 PANC10_27 
                 20 
                 0 
                 C 
                 PANC10 
                 267.4496 
                 25.71631 
                 0 
                 161.1555 
                 330.8832 
                 22.28747 
                 865.7824 
                 20482.18 
               
               
                 LNCaP_16 
                 20 
                 0 
                 C 
                 LNCaP 
                 166.4081 
                 147.7803 
                 12.41851 
                 409.811 
                 294.3188 
                 33.52999 
                 1435.58 
                 11798.83 
               
               
                 22RV1_27 
                 20 
                 0 
                 C 
                 22RV1 
                 220.7981 
                 118.5017 
                 4.051341 
                 430.455 
                 238.0163 
                 16.20536 
                 751.5237 
                 8691.139 
               
               
                 PC3_27 
                 20 
                 0 
                 C 
                 PC3 
                 699.4571 
                 410.7506 
                 11.15457 
                 398.9399 
                 359.5708 
                 14.43532 
                 812.3151 
                 17792.19 
               
               
                 DU145_27 
                 20 
                 0 
                 C 
                 DU145 
                 996.1885 
                 0 
                 110.6876 
                 0 
                 1217.564 
                 221.3752 
                 1771.002 
                 19591.71 
               
               
                 PC3_PSI_1 
                 20 
                 0 
                 A 
                 PC3 
                 1664.168 
                 254.2092 
                 11.80753 
                 548.0084 
                 587.5984 
                 77.7908 
                 2127.44 
                 18376.69 
               
               
                 PC3_PSI_2 
                 20 
                 0 
                 B 
                 PC3 
                 1500.024 
                 210.9101 
                 5.913367 
                 488.1813 
                 424.4483 
                 96.585 
                 2270.733 
                 21171.83 
               
               
                 PC3_PSI_3 
                 20 
                 0 
                 C 
                 PC3 
                 1481.33 
                 225.2641 
                 12.73578 
                 491.1234 
                 602.5615 
                 61.29093 
                 2450.841 
                 18856.11 
               
               
                 PANC10_4 
                 10 
                 2 
                 A 
                 PANC10 
                 388.3242 
                 57.908 
                 13.62541 
                 170.3176 
                 530.2556 
                 40.87623 
                 1196.765 
                 12772.69 
               
               
                 PC3_2 
                 10 
                 2 
                 A 
                 PC3 
                 1127.084 
                 485.2885 
                 36.79082 
                 395.9393 
                 637.1236 
                 50.80638 
                 873.0521 
                 21860.76 
               
               
                 DU145_2 
                 10 
                 2 
                 A 
                 DU145 
                 414.3253 
                 80.87098 
                 69.79276 
                 168.3889 
                 1087.881 
                 86.41008 
                 4175.38 
                 18361.04 
               
               
                 PANC10_11 
                 10 
                 5 
                 B 
                 PANC10 
                 246.0487 
                 33.96941 
                 4.590461 
                 169.8471 
                 377.3359 
                 6.426645 
                 943.7988 
                 18659.31 
               
               
                 LNCaP_2 
                 10 
                 5 
                 B 
                 LNCaP 
                 247.6243 
                 405.2033 
                 14.06956 
                 419.2729 
                 478.3651 
                 66.12694 
                 2207.514 
                 15593.29 
               
               
                 22RV1_11 
                 10 
                 5 
                 B 
                 22RV1 
                 211.1379 
                 131.6928 
                 6.441494 
                 396.5097 
                 334.242 
                 19.32448 
                 704.9857 
                 10325.71 
               
               
                 DU145_11 
                 10 
                 5 
                 B 
                 DU145 
                 424.3177 
                 102.2594 
                 22.33251 
                 202.168 
                 983.8058 
                 75.22529 
                 3838.841 
                 24570.46 
               
               
                 PANC10_19 
                 20 
                 2 
                 C 
                 PANC10 
                 234.8746 
                 38.70092 
                 6.672573 
                 146.7966 
                 334.9632 
                 33.36287 
                 914.1425 
                 20149.84 
               
               
                 PANC10_20 
                 20 
                 5 
                 C 
                 PANC10 
                 253.3356 
                 45.50203 
                 5.53403 
                 153.1082 
                 348.029 
                 33.20418 
                 1054.54 
                 21815.15 
               
               
                 LNCaP_11 
                 20 
                 2 
                 C 
                 LNCaP 
                 161.5547 
                 163.0369 
                 4.446461 
                 339.4132 
                 250.484 
                 34.08953 
                 1551.815 
                 10816.76 
               
               
                 LNCaP_12 
                 20 
                 5 
                 C 
                 LNCaP 
                 160.2608 
                 163.7447 
                 0 
                 369.2966 
                 280.4564 
                 27.87144 
                 1477.186 
                 10847.22 
               
               
                 22RV1_19 
                 20 
                 2 
                 C 
                 22RV1 
                 197.8738 
                 119.4952 
                 6.424474 
                 409.8815 
                 267.2581 
                 23.12811 
                 1029.201 
                 8685.889 
               
               
                 PC3_19 
                 20 
                 5 
                 C 
                 PC3 
                 670.3506 
                 441.7176 
                 16.96196 
                 342.0661 
                 474.228 
                 29.68342 
                 804.9861 
                 18424.92 
               
               
                 PC3_22 
                 20 
                 2 
                 C 
                 PC3 
                 616.4342 
                 412.4344 
                 10.13664 
                 311.7016 
                 456.1486 
                 35.47823 
                 866.0488 
                 20432.29 
               
               
                 DU145_19 
                 20 
                 2 
                 C 
                 DU145 
                 283.305 
                 110.0012 
                 31.13242 
                 176.4171 
                 1270.203 
                 53.96286 
                 2028.796 
                 17877.27 
               
               
                 DU145_20 
                 20 
                 5 
                 C 
                 DU145 
                 272.9211 
                 81.08527 
                 29.66534 
                 288.7427 
                 812.8303 
                 55.3753 
                 2099.317 
                 18893.86 
               
               
                 LNCaP_23 
                 10 
                 2 
                 A 
                 LNCaP 
                 210.4404 
                 134.5439 
                 3.449843 
                 322.5603 
                 260.4631 
                 36.22335 
                 1540.355 
                 12221.07 
               
               
                 22RV1_5 
                 10 
                 2 
                 A 
                 22RV1 
                 231.5576 
                 109.639 
                 8.771119 
                 511.3563 
                 292.0783 
                 16.66513 
                 735.0198 
                 9873.649 
               
               
                 PC3_5 
                 10 
                 2 
                 A 
                 PC3 
                 932.2809 
                 406.043 
                 18.49152 
                 375.2238 
                 530.8608 
                 29.27824 
                 976.1983 
                 24877.26 
               
               
                 DU145_5 
                 10 
                 2 
                 A 
                 DU145 
                 531.7783 
                 186.5714 
                 121.7204 
                 222.4889 
                 1420.736 
                 130.6997 
                 4202.346 
                 19979.1 
               
               
                 PANC10_14 
                 10 
                 5 
                 B 
                 PANC10 
                 244.5717 
                 46.2249 
                 8.404527 
                 179.0164 
                 401.7364 
                 42.02264 
                 1121.164 
                 21281.94 
               
               
                 LNCaP_5 
                 10 
                 5 
                 B 
                 LNCaP 
                 236.4201 
                 149.1802 
                 3.489596 
                 343.7252 
                 305.3396 
                 28.78916 
                 2145.229 
                 14346.6 
               
               
                 22RV1_14 
                 10 
                 5 
                 B 
                 22RV1 
                 212.7266 
                 123.0226 
                 4.271619 
                 423.7446 
                 325.4974 
                 22.21242 
                 609.1329 
                 10182.69 
               
               
                 PC3_14 
                 10 
                 5 
                 B 
                 PC3 
                 1372.075 
                 660.5132 
                 42.33261 
                 462.546 
                 750.1588 
                 115.1696 
                 1169.127 
                 29175.88 
               
               
                 DU145_14 
                 10 
                 5 
                 B 
                 DU145 
                 718.0632 
                 286.0285 
                 113.6933 
                 319.5381 
                 1571.362 
                 183.1061 
                 3811.719 
                 26951.31 
               
               
                 PANC10_22 
                 20 
                 2 
                 C 
                 PANC10 
                 241.9556 
                 31.37496 
                 1.898129 
                 153.1902 
                 385.2086 
                 11.50043 
                 835.9584 
                 16977.98 
               
               
                 PANC10_23 
                 20 
                 5 
                 C 
                 PANC10 
                 224.4627 
                 34.78357 
                 1.086987 
                 107.6117 
                 385.3367 
                 7.608906 
                 980.4619 
                 18153.76 
               
               
                 LNCaP_14 
                 20 
                 2 
                 C 
                 LNCaP 
                 185.8022 
                 182.617 
                 0 
                 425.7525 
                 285.6046 
                 35.03699 
                 1472.615 
                 12031.49 
               
               
                 LNCaP_15 
                 20 
                 5 
                 C 
                 LNCaP 
                 210.3339 
                 161.9813 
                 3.626446 
                 388.0297 
                 233.3014 
                 20.54986 
                 1646.407 
                 13451.7 
               
               
                 22RV1_22 
                 20 
                 2 
                 C 
                 22RV1 
                 185.5875 
                 131.6668 
                 7.523819 
                 500.334 
                 259.5718 
                 35.11116 
                 852.6995 
                 8938.298 
               
               
                 22RV1_23 
                 20 
                 5 
                 C 
                 22RV1 
                 195.4212 
                 109.9976 
                 5.850934 
                 439.9902 
                 320.6312 
                 19.89317 
                 788.7058 
                 9155.541 
               
               
                 PC3_20 
                 20 
                 5 
                 C 
                 PC3 
                 961.2017 
                 488.5887 
                 13.31304 
                 398.7256 
                 536.5156 
                 34.61391 
                 896.6334 
                 22240.1 
               
               
                 PC3_23 
                 20 
                 2 
                 C 
                 PC3 
                 975.2113 
                 431.7469 
                 17.56259 
                 385.4012 
                 456.1394 
                 34.63732 
                 887.8863 
                 22027.38 
               
               
                 DU145_22 
                 20 
                 2 
                 C 
                 DU145 
                 352.0325 
                 123.6235 
                 72.99672 
                 243.7148 
                 1377.519 
                 65.93252 
                 2081.584 
                 15409.37 
               
               
                 DU145_23 
                 20 
                 5 
                 C 
                 DU145 
                 321.1689 
                 97.3239 
                 30.08193 
                 139.7925 
                 1235.129 
                 32.73622 
                 2099.542 
                 16100.91 
               
               
                 PANC10_6 
                 10 
                 2 
                 A 
                 PANC10 
                 275.0814 
                 24.77833 
                 4.619688 
                 152.4497 
                 294.4001 
                 27.71813 
                 1201.959 
                 24722.47 
               
               
                 LNCaP_24 
                 10 
                 2 
                 A 
                 LNCaP 
                 191.2567 
                 137.5476 
                 7.859865 
                 328.8044 
                 331.4243 
                 32.74944 
                 1923.047 
                 15162.99 
               
               
                 22RV1_8 
                 10 
                 2 
                 A 
                 22RV1 
                 210.2406 
                 129.0285 
                 4.553949 
                 465.2618 
                 245.1542 
                 15.93882 
                 881.9481 
                 10532.52 
               
               
                 PC3_8 
                 10 
                 2 
                 A 
                 PC3 
                 1268.069 
                 676.1431 
                 25.66617 
                 421.0856 
                 741.9127 
                 52.13441 
                 1305.766 
                 32049.02 
               
               
                 DU145_8 
                 10 
                 2 
                 A 
                 DU145 
                 484.3028 
                 162.0973 
                 97.45725 
                 226.7373 
                 1511.582 
                 72.5957 
                 3500.505 
                 20059.29 
               
               
                 PANC10_17 
                 10 
                 5 
                 B 
                 PANC10 
                 322.1212 
                 23.81882 
                 3.402689 
                 168.4331 
                 326.6581 
                 23.25171 
                 1291.32 
                 21974 
               
               
                 LNCaP_8 
                 10 
                 5 
                 B 
                 LNCaP 
                 177.4626 
                 216.7482 
                 10.83741 
                 356.2799 
                 376.6001 
                 40.64029 
                 1574.134 
                 14511.29 
               
               
                 22RV1_17 
                 10 
                 5 
                 B 
                 22RV1 
                 239.3598 
                 120.3411 
                 5.28972 
                 435.0795 
                 323.9953 
                 15.86916 
                 756.4299 
                 10143.04 
               
               
                 PC3_17 
                 10 
                 5 
                 B 
                 PC3 
                 972.7494 
                 524.2362 
                 29.85234 
                 401.9144 
                 527.1486 
                 72.81059 
                 1108.905 
                 25722.52 
               
               
                 DU145_17 
                 10 
                 5 
                 B 
                 DU145 
                 321.8763 
                 162.586 
                 56.02626 
                 221.9079 
                 1329.25 
                 71.40601 
                 2352.004 
                 19793.75 
               
               
                 PANC10_26 
                 20 
                 5 
                 C 
                 PANC10 
                 210.1558 
                 32.4252 
                 2.634548 
                 134.3619 
                 362.9596 
                 7.29567 
                 992.0085 
                 20848.8 
               
               
                 LNCaP_17 
                 20 
                 2 
                 C 
                 LNCaP 
                 144.6707 
                 201.7776 
                 6.345206 
                 418.7836 
                 253.8082 
                 31.72603 
                 1463.205 
                 10600.3 
               
               
                 LNCaP_18 
                 20 
                 5 
                 C 
                 LNCaP 
                 200.3051 
                 120.9572 
                 0.967658 
                 311.5857 
                 244.8174 
                 20.32081 
                 1815.326 
                 11864.45 
               
               
                 22RV1_25 
                 20 
                 2 
                 C 
                 22RV1 
                 208.1508 
                 121.137 
                 5.118463 
                 380.4724 
                 226.9185 
                 23.88616 
                 759.2387 
                 9999.771 
               
               
                 22RV1_26 
                 20 
                 5 
                 C 
                 22RV1 
                 192.6104 
                 118.7764 
                 1.605087 
                 393.2463 
                 287.3105 
                 16.05087 
                 828.2248 
                 10298.24 
               
               
                 PC3_21 
                 20 
                 5 
                 C 
                 PC3 
                 675.8017 
                 388.9932 
                 10.86022 
                 359.8681 
                 427.004 
                 23.69502 
                 776.9992 
                 21501.26 
               
               
                 PC3_24 
                 20 
                 2 
                 C 
                 PC3 
                 710.7665 
                 408.105 
                 10.25144 
                 335.8567 
                 405.6641 
                 20.01472 
                 809.8638 
                 21619.31 
               
               
                 DU145_25 
                 20 
                 2 
                 C 
                 DU145 
                 327.3233 
                 93.92135 
                 39.62393 
                 168.0582 
                 1223.67 
                 43.63578 
                 1988.066 
                 17555.27 
               
               
                 DU145_26 
                 20 
                 5 
                 C 
                 DU145 
                 0 
                 0 
                 0 
                 241.1813 
                 482.3626 
                 0 
                 1688.269 
                 6994.258 
               
               
                 PC3_PSI_4 
                 20 
                 0.5 
                 A 
                 PC3 
                 1470.105 
                 299.8807 
                 7.468294 
                 589.4208 
                 498.0778 
                 88.47056 
                 2114.676 
                 20392.47 
               
               
                 PC3_PSI_7 
                 20 
                 2 
                 A 
                 PC3 
                 913.9944 
                 249.5563 
                 6.27251 
                 402.3367 
                 398.7524 
                 49.28401 
                 1836.053 
                 21830.13 
               
               
                 PC3_PSI_10 
                 20 
                 5 
                 A 
                 PC3 
                 1082.442 
                 231.3616 
                 11.47627 
                 386.0616 
                 405.8008 
                 42.23267 
                 1590.611 
                 23326.66 
               
               
                 PC3_PSI_5 
                 20 
                 0.5 
                 B 
                 PC3 
                 1393.246 
                 294.2318 
                 10.64655 
                 573.4617 
                 486.8376 
                 80.33304 
                 2149.635 
                 19581.9 
               
               
                 PC3_PSI_8 
                 20 
                 2 
                 B 
                 PC3 
                 929.2346 
                 237.8557 
                 8.875211 
                 387.8467 
                 434.8853 
                 56.80135 
                 1786.58 
                 20405 
               
               
                 PC3_PSI_11 
                 20 
                 5 
                 B 
                 PC3 
                 955.0823 
                 239.8718 
                 12.81452 
                 364.4129 
                 430.8883 
                 49.25581 
                 1538.143 
                 23672.02 
               
               
                 PC3_PSI_6 
                 20 
                 0.5 
                 C 
                 PC3 
                 1275.758 
                 348.6979 
                 1.400393 
                 639.9797 
                 494.3388 
                 113.4318 
                 1960.55 
                 20218.88 
               
               
                 PC3_PSI_9 
                 20 
                 2 
                 C 
                 PC3 
                 867.4324 
                 285.8406 
                 11.47535 
                 366.6897 
                 484.0513 
                 45.90142 
                 1520.484 
                 22023.81 
               
               
                 PC3_PSI_12 
                 20 
                 5 
                 C 
                 PC3 
                 1123.57 
                 261.2875 
                 9.009913 
                 433.8106 
                 426.8029 
                 52.05727 
                 1841.359 
                 19512.13 
               
               
                 PANC10_1 
                 1 
                 2 
                 A 
                 PANC10 
                 731.0598 
                 187.9868 
                 26.10928 
                 313.3113 
                 1096.59 
                 219.3179 
                 1921.643 
                 20991.86 
               
               
                 LNCaP_19 
                 1 
                 2 
                 A 
                 LNCaP 
                 702.2483 
                 1298.139 
                 193.7739 
                 971.7834 
                 1169.928 
                 273.906 
                 5826.33 
                 49116.59 
               
               
                 22RV1_1 
                 1 
                 2 
                 A 
                 22RV1 
                 214.5837 
                 163.0836 
                 11.44447 
                 504.9871 
                 266.0838 
                 58.65289 
                 826.8627 
                 10547.51 
               
               
                 PC3_1 
                 1 
                 2 
                 A 
                 PC3 
                 2573.914 
                 1346.416 
                 88.59052 
                 662.4336 
                 1702.375 
                 122.9094 
                 1581.86 
                 31345.88 
               
               
                 DU145_1 
                 1 
                 2 
                 A 
                 DU145 
                 1051.173 
                 659.8602 
                 338.699 
                 599.574 
                 3639.096 
                 358.429 
                 6371.707 
                 26577.46 
               
               
                 PANC10_10 
                 1 
                 5 
                 B 
                 PANC10 
                 804.5866 
                 418.4743 
                 147.303 
                 571.3569 
                 1063.483 
                 446.3726 
                 2510.846 
                 31510.56 
               
               
                 LNCaP_3 
                 1 
                 5 
                 B 
                 LNCaP 
                 604.2898 
                 627.438 
                 92.59279 
                 855.265 
                 741.9607 
                 159.6007 
                 4498.061 
                 44259.36 
               
               
                 22RV1_10 
                 1 
                 5 
                 B 
                 22RV1 
                 580.0314 
                 467.6222 
                 151.7524 
                 784.6161 
                 753.1415 
                 229.3147 
                 2421.294 
                 24631.1 
               
               
                 DU145_10 
                 1 
                 5 
                 B 
                 DU145 
                 1203.968 
                 624.6599 
                 214.7803 
                 558.7712 
                 2454.143 
                 245.5855 
                 6173.865 
                 31692.51 
               
               
                 PANC10_2 
                 1 
                 2 
                 A 
                 PANC10 
                 1183.027 
                 307.4562 
                 149.4509 
                 695.9279 
                 1512.121 
                 346.7059 
                 5590.067 
                 78193.51 
               
               
                 22RV1_4 
                 1 
                 2 
                 A 
                 22RV1 
                 532.976 
                 494.5483 
                 162.0648 
                 826.1964 
                 652.4362 
                 311.5988 
                 2659.868 
                 25173.51 
               
               
                 PC3_4 
                 1 
                 2 
                 A 
                 PC3 
                 4049.774 
                 3173.175 
                 378.6605 
                 1172.901 
                 2545.545 
                 256.5425 
                 3821.631 
                 65465.66 
               
               
                 DU145_4 
                 1 
                 2 
                 A 
                 DU145 
                 1195.26 
                 788.3113 
                 406.9489 
                 642.1021 
                 3689.346 
                 383.7992 
                 8898.048 
                 36787.45 
               
               
                 PANC10_13 
                 1 
                 5 
                 B 
                 PANC10 
                 772.2797 
                 374.3285 
                 86.31362 
                 516.9731 
                 1433.715 
                 536.9616 
                 3733.291 
                 68947.32 
               
               
                 LNCaP_6 
                 1 
                 5 
                 B 
                 LNCaP 
                 526.9173 
                 551.0878 
                 56.80072 
                 831.4659 
                 807.2953 
                 123.2696 
                 4291.476 
                 43798.19 
               
               
                 22RV1_13 
                 1 
                 5 
                 B 
                 22RV1 
                 526.6807 
                 412.5491 
                 108.8962 
                 703.637 
                 692.1191 
                 216.7453 
                 2371.634 
                 24520.49 
               
               
                 PC3_13 
                 1 
                 5 
                 B 
                 PC3 
                 4524.925 
                 3367.721 
                 270.9948 
                 1122.156 
                 2841.377 
                 296.029 
                 4216.379 
                 64623.19 
               
               
                 DU145_13 
                 1 
                 5 
                 B 
                 DU145 
                 1366.53 
                 885.2882 
                 312.0682 
                 808.0923 
                 2534.322 
                 594.572 
                 6794.874 
                 49986.75 
               
               
                 PANC10_3 
                 1 
                 2 
                 A 
                 PANC10 
                 867.7696 
                 348.604 
                 80.79235 
                 647.8349 
                 1524.581 
                 323.1694 
                 3840.629 
                 65821.82 
               
               
                 LNCaP_21 
                 1 
                 2 
                 A 
                 LNCaP 
                 818.9263 
                 785.0397 
                 141.1942 
                 1016.598 
                 1157.792 
                 200.4958 
                 5475.511 
                 50954.16 
               
               
                 22RV1_7 
                 1 
                 2 
                 A 
                 22RV1 
                 719.2968 
                 771.1298 
                 254.6183 
                 940.2691 
                 1096.678 
                 405.5706 
                 4000.236 
                 34090.67 
               
               
                 PC3_7 
                 1 
                 2 
                 A 
                 PC3 
                 4536.24 
                 3476.616 
                 242.0746 
                 1253.534 
                 2829.208 
                 184.5271 
                 4467.433 
                 75690.53 
               
               
                 DU145_7 
                 1 
                 2 
                 A 
                 DU145 
                 1027.857 
                 677.8808 
                 337.3639 
                 457.1754 
                 3470.329 
                 259.5916 
                 6680.016 
                 32923.99 
               
               
                 PANC10_16 
                 1 
                 5 
                 B 
                 PANC10 
                 758.2926 
                 364.7887 
                 74.44668 
                 503.0468 
                 1655.907 
                 450.9342 
                 3356.482 
                 61379.16 
               
               
                 LNCaP_9 
                 1 
                 5 
                 B 
                 LNCaP 
                 570.9571 
                 438.3332 
                 47.20511 
                 745.1664 
                 690.0938 
                 122.5085 
                 4372.093 
                 45783.34 
               
               
                 22RV1_16 
                 1 
                 5 
                 B 
                 22RV1 
                 550.355 
                 384.2356 
                 128.3036 
                 709.0463 
                 815.7409 
                 237.0241 
                 1979.927 
                 29000.67 
               
               
                 PC3_16 
                 1 
                 5 
                 B 
                 PC3 
                 3915.276 
                 2814.212 
                 252.5061 
                 1071.863 
                 2282.289 
                 302.3202 
                 3744.648 
                 70023.21 
               
               
                 DU145_16 
                 1 
                 5 
                 B 
                 DU145 
                 961.737 
                 931.1199 
                 90.05028 
                 1010.364 
                 1732.567 
                 781.6364 
                 3632.628 
                 30377.56 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 12 
               
               
                   
               
               
                 Oxygen-Sensitive Candidates 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 O2 
                 PSI 
                 Expt 
                 CellLine 
                 P4HA1 
                 PDK1 
                 HK2 
                 SLC2A1 
               
               
                   
               
               
                 LNCaP_25 
                 20 
                 0 
                 A 
                 LNCaP 
                 649.5917 
                 517.6434 
                 827.2145 
                 779.8484 
               
               
                 22RV1_3 
                 20 
                 0 
                 A 
                 22RV1 
                 630.8675 
                 163.4094 
                 1745.266 
                 1078.234 
               
               
                 PC3_3 
                 20 
                 0 
                 A 
                 PC3 
                 2446.465 
                 314.3372 
                 1463.796 
                 1660.33 
               
               
                 DU145_3 
                 20 
                 0 
                 A 
                 DU145 
                 970.0978 
                 260.2968 
                 407.9442 
                 4884.393 
               
               
                 PANC10_12 
                 20 
                 0 
                 B 
                 PANC10 
                 380.7663 
                 162.59 
                 1438.296 
                 993.6055 
               
               
                 LNCaP_1 
                 20 
                 0 
                 B 
                 LNCaP 
                 743.5561 
                 615.2785 
                 860.4817 
                 648.1993 
               
               
                 22RV1_12 
                 20 
                 0 
                 B 
                 22RV1 
                 691.4687 
                 140.7887 
                 1638.674 
                 762.7541 
               
               
                 DU145_12 
                 20 
                 0 
                 B 
                 DU145 
                 1446.568 
                 546.0028 
                 391.9439 
                 3900.182 
               
               
                 PANC10_21 
                 20 
                 0 
                 C 
                 PANC10 
                 647.8829 
                 346.2442 
                 1475.071 
                 673.9396 
               
               
                 LNCaP_10 
                 20 
                 0 
                 C 
                 LNCaP 
                 632.5891 
                 510.6683 
                 658.5723 
                 1055.315 
               
               
                 22RV1_21 
                 20 
                 0 
                 C 
                 22RV1 
                 615.9897 
                 192.7907 
                 1689.269 
                 945.1445 
               
               
                 PC3_25 
                 20 
                 0 
                 C 
                 PC3 
                 1517.876 
                 222.2692 
                 1004.756 
                 1670.738 
               
               
                 DU145_21 
                 20 
                 0 
                 C 
                 DU145 
                 1021.792 
                 319.0576 
                 492.7219 
                 3892.301 
               
               
                 PANC10_8 
                 20 
                 0 
                 A 
                 PANC10 
                 610.8968 
                 233.3287 
                 1392.699 
                 760.5908 
               
               
                 LNCaP_26 
                 20 
                 0 
                 A 
                 LNCaP 
                 806.4939 
                 585.7116 
                 718.9108 
                 689.7165 
               
               
                 22RV1_6 
                 20 
                 0 
                 A 
                 22RV1 
                 688.0363 
                 169.4418 
                 1500.33 
                 909.8509 
               
               
                 PC3_6 
                 20 
                 0 
                 A 
                 PC3 
                 2284.137 
                 313.7671 
                 1392.57 
                 1420.363 
               
               
                 DU145_6 
                 20 
                 0 
                 A 
                 DU145 
                 1002.637 
                 309.6547 
                 439.7327 
                 4949.871 
               
               
                 PANC10_15 
                 20 
                 0 
                 B 
                 PANC10 
                 557.7053 
                 237.8784 
                 1526.291 
                 630.5484 
               
               
                 LNCaP_4 
                 20 
                 0 
                 B 
                 LNCaP 
                 676.7228 
                 544.6136 
                 812.8762 
                 1005.648 
               
               
                 22RV1_15 
                 20 
                 0 
                 B 
                 22RV1 
                 695.9886 
                 139.3927 
                 1692.208 
                 884.12 
               
               
                 PC3_15 
                 20 
                 0 
                 B 
                 PC3 
                 3292.687 
                 381.8236 
                 1419.364 
                 2126.063 
               
               
                 DU145_15 
                 20 
                 0 
                 B 
                 DU145 
                 1396.275 
                 404.2644 
                 595.8111 
                 4661.642 
               
               
                 PANC10_24 
                 20 
                 0 
                 C 
                 PANC10 
                 442.4396 
                 237.8169 
                 1573.321 
                 888.9716 
               
               
                 LNCaP_13 
                 20 
                 0 
                 C 
                 LNCaP 
                 701.3473 
                 571.904 
                 890.8053 
                 1093.208 
               
               
                 22RV1_24 
                 20 
                 0 
                 C 
                 22RV1 
                 732.7024 
                 187.3962 
                 1635.074 
                 869.451 
               
               
                 PC3_26 
                 20 
                 0 
                 C 
                 PC3 
                 1839.411 
                 267.9427 
                 989.3862 
                 2629.381 
               
               
                 DU145_24 
                 20 
                 0 
                 C 
                 DU145 
                 889.4556 
                 210.6179 
                 708.0002 
                 3149.548 
               
               
                 PANC10_9 
                 20 
                 0 
                 A 
                 PANC10 
                 454.5376 
                 347.3966 
                 1451.274 
                 811.6744 
               
               
                 LNCaP_27 
                 20 
                 0 
                 A 
                 LNCaP 
                 683.3315 
                 522.0734 
                 905.0615 
                 776.055 
               
               
                 22RV1_9 
                 20 
                 0 
                 A 
                 22RV1 
                 734.8624 
                 125.214 
                 1239.824 
                 927.8151 
               
               
                 PC3_9 
                 20 
                 0 
                 A 
                 PC3 
                 2725.102 
                 467.9921 
                 1644.13 
                 1780.161 
               
               
                 DU145_9 
                 20 
                 0 
                 A 
                 DU145 
                 1434.512 
                 503.8392 
                 614.9478 
                 4513.651 
               
               
                 PANC10_18 
                 20 
                 0 
                 B 
                 PANC10 
                 734.739 
                 325.4549 
                 1568.101 
                 761.0384 
               
               
                 LNCaP_7 
                 20 
                 0 
                 B 
                 LNCaP 
                 860.5564 
                 654.5666 
                 808.2747 
                 734.0347 
               
               
                 22RV1_18 
                 20 
                 0 
                 B 
                 22RV1 
                 647.1424 
                 179.7618 
                 1580.62 
                 837.1763 
               
               
                 PC3_18 
                 20 
                 0 
                 B 
                 PC3 
                 2106.253 
                 341.0059 
                 1355.905 
                 2117.755 
               
               
                 DU145_18 
                 20 
                 0 
                 B 
                 DU145 
                 1187.261 
                 348.1306 
                 602.2207 
                 3117.803 
               
               
                 PANC10_27 
                 20 
                 0 
                 C 
                 PANC10 
                 454.3215 
                 276.0217 
                 1273.815 
                 1016.651 
               
               
                 LNCaP_16 
                 20 
                 0 
                 C 
                 LNCaP 
                 706.6135 
                 599.8143 
                 702.8879 
                 1046.881 
               
               
                 22RV1_27 
                 20 
                 0 
                 C 
                 22RV1 
                 679.6124 
                 180.2847 
                 1556.728 
                 803.1783 
               
               
                 PC3_27 
                 20 
                 0 
                 C 
                 PC3 
                 1478.965 
                 232.9337 
                 1005.224 
                 1622.662 
               
               
                 DU145_27 
                 20 
                 0 
                 C 
                 DU145 
                 332.0628 
                 110.6876 
                 553.4381 
                 2324.44 
               
               
                 PC3_PSI_1 
                 20 
                 0 
                 A 
                 PC3 
                 5714.151 
                 404.2344 
                 1986.444 
                 2360.812 
               
               
                 PC3_PSI_2 
                 20 
                 0 
                 B 
                 PC3 
                 6109.165 
                 376.4844 
                 1645.887 
                 2259.563 
               
               
                 PC3_PSI_3 
                 20 
                 0 
                 C 
                 PC3 
                 5512.204 
                 439.3843 
                 2096.627 
                 2465.965 
               
               
                 PANC10_4 
                 10 
                 2 
                 A 
                 PANC10 
                 465.5349 
                 346.3125 
                 2496.856 
                 1017.364 
               
               
                 PC3_2 
                 10 
                 2 
                 A 
                 PC3 
                 3003.416 
                 527.9191 
                 1531.199 
                 1958.673 
               
               
                 DU145_2 
                 10 
                 2 
                 A 
                 DU145 
                 653.6147 
                 435.3739 
                 301.3275 
                 5786.152 
               
               
                 PANC10_11 
                 10 
                 5 
                 B 
                 PANC10 
                 370.9092 
                 186.3727 
                 1402.845 
                 747.3271 
               
               
                 LNCaP_2 
                 10 
                 5 
                 B 
                 LNCaP 
                 938.4397 
                 1060.845 
                 896.231 
                 1066.473 
               
               
                 22RV1_11 
                 10 
                 5 
                 B 
                 22RV1 
                 587.6074 
                 191.8134 
                 1524.487 
                 815.2069 
               
               
                 DU145_11 
                 10 
                 5 
                 B 
                 DU145 
                 1067.259 
                 684.08 
                 218.6235 
                 4109.182 
               
               
                 PANC10_19 
                 20 
                 2 
                 C 
                 PANC10 
                 358.9844 
                 322.9525 
                 1034.249 
                 899.4629 
               
               
                 PANC10_20 
                 20 
                 5 
                 C 
                 PANC10 
                 426.7352 
                 347.4141 
                 1170.755 
                 843.0173 
               
               
                 LNCaP_11 
                 20 
                 2 
                 C 
                 LNCaP 
                 641.7725 
                 672.8978 
                 542.4682 
                 1050.847 
               
               
                 LNCaP_12 
                 20 
                 5 
                 C 
                 LNCaP 
                 646.2691 
                 630.5914 
                 674.1405 
                 921.4995 
               
               
                 22RV1_19 
                 20 
                 2 
                 C 
                 22RV1 
                 481.8356 
                 168.3212 
                 1372.268 
                 898.1415 
               
               
                 PC3_19 
                 20 
                 5 
                 C 
                 PC3 
                 1454.841 
                 293.3005 
                 1160.127 
                 1879.95 
               
               
                 PC3_22 
                 20 
                 2 
                 C 
                 PC3 
                 1389.986 
                 332.6084 
                 1096.657 
                 2104.619 
               
               
                 DU145_19 
                 20 
                 2 
                 C 
                 DU145 
                 771.0463 
                 292.6448 
                 372.5513 
                 4544.296 
               
               
                 DU145_20 
                 20 
                 5 
                 C 
                 DU145 
                 777.2319 
                 282.8096 
                 354.0064 
                 3331.418 
               
               
                 LNCaP_23 
                 10 
                 2 
                 A 
                 LNCaP 
                 562.3244 
                 757.2405 
                 726.1919 
                 867.6354 
               
               
                 22RV1_5 
                 10 
                 2 
                 A 
                 22RV1 
                 526.2672 
                 200.8586 
                 1475.302 
                 942.0182 
               
               
                 PC3_5 
                 10 
                 2 
                 A 
                 PC3 
                 2330.702 
                 503.894 
                 1410.749 
                 1630.336 
               
               
                 DU145_5 
                 10 
                 2 
                 A 
                 DU145 
                 895.9417 
                 565.7004 
                 529.7829 
                 4733.126 
               
               
                 PANC10_14 
                 10 
                 5 
                 B 
                 PANC10 
                 469.8131 
                 350.4688 
                 1268.243 
                 837.9314 
               
               
                 LNCaP_5 
                 10 
                 5 
                 B 
                 LNCaP 
                 1092.243 
                 1116.671 
                 734.5599 
                 789.521 
               
               
                 22RV1_14 
                 10 
                 5 
                 B 
                 22RV1 
                 574.9599 
                 165.7388 
                 1411.343 
                 768.8914 
               
               
                 PC3_14 
                 10 
                 5 
                 B 
                 PC3 
                 3483.102 
                 719.0319 
                 1566.307 
                 2744.149 
               
               
                 DU145_14 
                 10 
                 5 
                 B 
                 DU145 
                 1287.727 
                 807.8211 
                 539.7442 
                 4438.828 
               
               
                 PANC10_22 
                 20 
                 2 
                 C 
                 PANC10 
                 305.3755 
                 231.9067 
                 1287.936 
                 823.788 
               
               
                 PANC10_23 
                 20 
                 5 
                 C 
                 PANC10 
                 376.0974 
                 271.2032 
                 1108.183 
                 748.3903 
               
               
                 LNCaP_14 
                 20 
                 2 
                 C 
                 LNCaP 
                 678.4435 
                 713.4805 
                 748.5175 
                 1030.937 
               
               
                 LNCaP_15 
                 20 
                 5 
                 C 
                 LNCaP 
                 724.0804 
                 889.6881 
                 620.1223 
                 956.173 
               
               
                 22RV1_22 
                 20 
                 2 
                 C 
                 22RV1 
                 605.6675 
                 225.7146 
                 1423.256 
                 872.7631 
               
               
                 22RV1_23 
                 20 
                 5 
                 C 
                 22RV1 
                 576.902 
                 196.5914 
                 1425.287 
                 903.3841 
               
               
                 PC3_20 
                 20 
                 5 
                 C 
                 PC3 
                 2397.013 
                 392.7347 
                 1262.742 
                 2501.521 
               
               
                 PC3_23 
                 20 
                 2 
                 C 
                 PC3 
                 2171.906 
                 408.3301 
                 1249.383 
                 2355.338 
               
               
                 DU145_22 
                 20 
                 2 
                 C 
                 DU145 
                 704.0651 
                 276.6811 
                 521.5733 
                 3496.778 
               
               
                 DU145_23 
                 20 
                 5 
                 C 
                 DU145 
                 755.5873 
                 307.8974 
                 391.9499 
                 3486.85 
               
               
                 PANC10_6 
                 10 
                 2 
                 A 
                 PANC10 
                 496.4065 
                 395.1933 
                 1267.474 
                 753.0091 
               
               
                 LNCaP_24 
                 10 
                 2 
                 A 
                 LNCaP 
                 818.736 
                 872.445 
                 791.2264 
                 755.857 
               
               
                 22RV1_8 
                 10 
                 2 
                 A 
                 22RV1 
                 557.8587 
                 231.4924 
                 1137.728 
                 997.3148 
               
               
                 PC3_8 
                 10 
                 2 
                 A 
                 PC3 
                 3033.42 
                 677.7473 
                 1817.486 
                 2119.063 
               
               
                 DU145_8 
                 10 
                 2 
                 A 
                 DU145 
                 782.6413 
                 521.0979 
                 348.0616 
                 3922.157 
               
               
                 PANC10_17 
                 10 
                 5 
                 B 
                 PANC10 
                 537.6248 
                 352.7454 
                 1374.119 
                 722.5042 
               
               
                 LNCaP_8 
                 10 
                 5 
                 B 
                 LNCaP 
                 732.88 
                 872.4116 
                 803.3231 
                 1007.879 
               
               
                 22RV1_17 
                 10 
                 5 
                 B 
                 22RV1 
                 677.0841 
                 207.6215 
                 1496.991 
                 763.0421 
               
               
                 PC3_17 
                 10 
                 5 
                 B 
                 PC3 
                 2249.119 
                 491.4714 
                 1362.286 
                 2311.736 
               
               
                 DU145_17 
                 10 
                 5 
                 B 
                 DU145 
                 949.1507 
                 449.3086 
                 342.7489 
                 3878.994 
               
               
                 PANC10_26 
                 20 
                 5 
                 C 
                 PANC10 
                 395.9928 
                 313.5112 
                 1105.091 
                 765.0321 
               
               
                 LNCaP_17 
                 20 
                 2 
                 C 
                 LNCaP 
                 540.6116 
                 719.5464 
                 697.9727 
                 1271.579 
               
               
                 LNCaP_18 
                 20 
                 5 
                 C 
                 LNCaP 
                 748.967 
                 979.2695 
                 737.3551 
                 769.2878 
               
               
                 22RV1_25 
                 20 
                 2 
                 C 
                 22RV1 
                 544.2632 
                 220.0939 
                 1337.625 
                 897.4372 
               
               
                 22RV1_26 
                 20 
                 5 
                 C 
                 22RV1 
                 598.6974 
                 218.2918 
                 1362.719 
                 882.7977 
               
               
                 PC3_21 
                 20 
                 5 
                 C 
                 PC3 
                 1519.443 
                 293.2259 
                 1094.414 
                 1690.245 
               
               
                 PC3_24 
                 20 
                 2 
                 C 
                 PC3 
                 1659.757 
                 289.4812 
                 1111.061 
                 1648.041 
               
               
                 DU145_25 
                 20 
                 2 
                 C 
                 DU145 
                 677.9484 
                 283.5227 
                 335.7317 
                 3924.363 
               
               
                 DU145_26 
                 20 
                 5 
                 C 
                 DU145 
                 723.5439 
                 0 
                 1447.088 
                 2411.813 
               
               
                 PC3_PSI_4 
                 20 
                 0.5 
                 A 
                 PC3 
                 5684.521 
                 567.5904 
                 2330.108 
                 2964.338 
               
               
                 PC3_PSI_7 
                 20 
                 2 
                 A 
                 PC3 
                 3168.962 
                 676.5351 
                 2363.84 
                 1947.166 
               
               
                 PC3_PSI_10 
                 20 
                 5 
                 A 
                 PC3 
                 2911.759 
                 659.1968 
                 2217.215 
                 2038.185 
               
               
                 PC3_PSI_5 
                 20 
                 0.5 
                 B 
                 PC3 
                 5978.036 
                 587.9798 
                 2240.13 
                 2297.235 
               
               
                 PC3_PSI_8 
                 20 
                 2 
                 B 
                 PC3 
                 2980.296 
                 670.966 
                 2292.467 
                 1868.232 
               
               
                 PC3_PSI_11 
                 20 
                 5 
                 B 
                 PC3 
                 2440.766 
                 653.941 
                 2114.396 
                 1842.888 
               
               
                 PC3_PSI_6 
                 20 
                 0.5 
                 C 
                 PC3 
                 5698.2 
                 504.1415 
                 2181.813 
                 3509.385 
               
               
                 PC3_PSI_9 
                 20 
                 2 
                 C 
                 PC3 
                 2633.072 
                 619.6691 
                 2340.451 
                 2198.052 
               
               
                 PC3_PSI_12 
                 20 
                 5 
                 C 
                 PC3 
                 3495.846 
                 623.3525 
                 2334.235 
                 2044.583 
               
               
                 PANC10_1 
                 1 
                 2 
                 A 
                 PANC10 
                 1154.03 
                 1091.368 
                 4140.931 
                 1968.64 
               
               
                 LNCaP_19 
                 1 
                 2 
                 A 
                 LNCaP 
                 2533.63 
                 3489.387 
                 2233.499 
                 2788.596 
               
               
                 22RV1_1 
                 1 
                 2 
                 A 
                 22RV1 
                 570.7928 
                 193.1254 
                 1821.101 
                 1194.516 
               
               
                 PC3_1 
                 1 
                 2 
                 A 
                 PC3 
                 5150.222 
                 1217.122 
                 2708.795 
                 3858.077 
               
               
                 DU145_1 
                 1 
                 2 
                 A 
                 DU145 
                 1531.27 
                 959.0991 
                 1274.78 
                 8591.336 
               
               
                 PANC10_10 
                 1 
                 5 
                 B 
                 PANC10 
                 1489.769 
                 1259.887 
                 3536.387 
                 2274.268 
               
               
                 LNCaP_3 
                 1 
                 5 
                 B 
                 LNCaP 
                 2708.339 
                 2442.744 
                 1751.953 
                 1772.665 
               
               
                 22RV1_10 
                 1 
                 5 
                 B 
                 22RV1 
                 1570.356 
                 460.8777 
                 3189.049 
                 1848.007 
               
               
                 DU145_10 
                 1 
                 5 
                 B 
                 DU145 
                 1849.165 
                 1211.669 
                 1052.509 
                 7289.696 
               
               
                 PANC10_2 
                 1 
                 2 
                 A 
                 PANC10 
                 3117.334 
                 2465.185 
                 3551.597 
                 2408.323 
               
               
                 22RV1_4 
                 1 
                 2 
                 A 
                 22RV1 
                 1187.918 
                 595.63 
                 2880.41 
                 1901.338 
               
               
                 PC3_4 
                 1 
                 2 
                 A 
                 PC3 
                 7777.686 
                 2626.01 
                 3321.799 
                 6459.001 
               
               
                 DU145_4 
                 1 
                 2 
                 A 
                 DU145 
                 1791.063 
                 1147.742 
                 1363.401 
                 8994.303 
               
               
                 PANC10_13 
                 1 
                 5 
                 B 
                 PANC10 
                 2011.562 
                 1618.153 
                 2430.41 
                 2528.535 
               
               
                 LNCaP_6 
                 1 
                 5 
                 B 
                 LNCaP 
                 2837.619 
                 2337.289 
                 1760.822 
                 1827.291 
               
               
                 22RV1_13 
                 1 
                 5 
                 B 
                 22RV1 
                 1331.884 
                 471.1855 
                 2938.103 
                 1777.94 
               
               
                 PC3_13 
                 1 
                 5 
                 B 
                 PC3 
                 8870.855 
                 2186.108 
                 3833.356 
                 8164.266 
               
               
                 DU145_13 
                 1 
                 5 
                 B 
                 DU145 
                 2156.555 
                 1241.703 
                 998.6182 
                 8236.958 
               
               
                 PANC10_3 
                 1 
                 2 
                 A 
                 PANC10 
                 2281.636 
                 1934.528 
                 2818.755 
                 2720.009 
               
               
                 LNCaP_21 
                 1 
                 2 
                 A 
                 LNCaP 
                 3128.863 
                 3035.675 
                 2180.038 
                 1843.996 
               
               
                 22RV1_7 
                 1 
                 2 
                 A 
                 22RV1 
                 1699.577 
                 959.3655 
                 3535.557 
                 2450.701 
               
               
                 PC3_7 
                 1 
                 2 
                 A 
                 PC3 
                 8564.561 
                 2882.376 
                 3866.938 
                 7705.728 
               
               
                 DU145_7 
                 1 
                 2 
                 A 
                 DU145 
                 2227.022 
                 982.6645 
                 873.3627 
                 7695.261 
               
               
                 PANC10_16 
                 1 
                 5 
                 B 
                 PANC10 
                 2214.257 
                 1636.763 
                 2495.027 
                 2823.656 
               
               
                 LNCaP_9 
                 1 
                 5 
                 B 
                 LNCaP 
                 2806.456 
                 2419.824 
                 1773.563 
                 1452.119 
               
               
                 22RV1_16 
                 1 
                 5 
                 B 
                 22RV1 
                 1160.135 
                 524.6943 
                 2679.52 
                 1509.931 
               
               
                 PC3_16 
                 1 
                 5 
                 B 
                 PC3 
                 7363.903 
                 1915.84 
                 3168.637 
                 7527.66 
               
               
                 DU145_16 
                 1 
                 5 
                 B 
                 DU145 
                 3322.855 
                 633.954 
                 520.4906 
                 5664.163 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                 TCAF2 
                 PGK1 
                 NDRG1 
                 RP11.61L23.2 
               
               
                   
                   
               
               
                   
                 LNCaP_25 
                 5.074935 
                 6913.754 
                 590.3842 
                 1.691645 
               
               
                   
                 22RV1_3 
                 13.39421 
                 5498.325 
                 375.038 
                 17.41248 
               
               
                   
                 PC3_3 
                 48.40442 
                 11756.44 
                 1036.321 
                 15.74602 
               
               
                   
                 DU145_3 
                 5.468421 
                 6583.978 
                 928.5378 
                 5.468421 
               
               
                   
                 PANC10_12 
                 8.337949 
                 8091.979 
                 3051.689 
                 15.28624 
               
               
                   
                 LNCaP_1 
                 13.6224 
                 6934.938 
                 532.4089 
                 1.1352 
               
               
                   
                 22RV1_12 
                 9.801746 
                 5673.429 
                 393.852 
                 18.71243 
               
               
                   
                 DU145_12 
                 4.531143 
                 12527.48 
                 1383.132 
                 3.398358 
               
               
                   
                 PANC10_21 
                 24.73173 
                 8389.797 
                 2002.828 
                 15.89897 
               
               
                   
                 LNCaP_10 
                 9.993509 
                 8129.72 
                 811.473 
                 7.994808 
               
               
                   
                 22RV1_21 
                 24.68661 
                 5088.968 
                 517.2432 
                 17.63329 
               
               
                   
                 PC3_25 
                 45.44538 
                 10514.41 
                 714.732 
                 23.96211 
               
               
                   
                 DU145_21 
                 7.067732 
                 6815.313 
                 1210.601 
                 6.058056 
               
               
                   
                 PANC10_8 
                 12.12097 
                 8984.668 
                 2512.071 
                 23.63589 
               
               
                   
                 LNCaP_26 
                 27.3697 
                 7249.322 
                 607.6074 
                 0 
               
               
                   
                 22RV1_6 
                 9.242278 
                 5890.412 
                 443.6294 
                 18.48456 
               
               
                   
                 PC3_6 
                 46.07768 
                 12791.31 
                 1051.741 
                 32.91263 
               
               
                   
                 DU145_6 
                 3.453398 
                 7278.613 
                 935.871 
                 13.81359 
               
               
                   
                 PANC10_15 
                 13.65809 
                 7513.087 
                 1914.409 
                 25.03983 
               
               
                   
                 LNCaP_4 
                 9.436374 
                 8479.256 
                 825.0087 
                 4.04416 
               
               
                   
                 22RV1_15 
                 11.69729 
                 5622.496 
                 439.6231 
                 6.823418 
               
               
                   
                 PC3_15 
                 75.93084 
                 14487.6 
                 1961.185 
                 35.2536 
               
               
                   
                 DU145_15 
                 9.073265 
                 9858.607 
                 2695.768 
                 7.056984 
               
               
                   
                 PANC10_24 
                 27.28301 
                 7942.54 
                 1993.479 
                 25.46415 
               
               
                   
                 LNCaP_13 
                 10.59082 
                 8366.744 
                 839.0279 
                 4.707029 
               
               
                   
                 22RV1_24 
                 24.47969 
                 5327.287 
                 538.5532 
                 15.19429 
               
               
                   
                 PC3_26 
                 50.04677 
                 11911.9 
                 878.5134 
                 23.09851 
               
               
                   
                 DU145_24 
                 16.20138 
                 5913.503 
                 959.1215 
                 1.620138 
               
               
                   
                 PANC10_9 
                 45.45376 
                 8035.576 
                 1733.736 
                 6.493395 
               
               
                   
                 LNCaP_27 
                 16.12582 
                 7077.218 
                 713.5674 
                 6.047182 
               
               
                   
                 22RV1_9 
                 10.26344 
                 5603.839 
                 504.9613 
                 16.42151 
               
               
                   
                 PC3_9 
                 82.85027 
                 13963.07 
                 1870.289 
                 48.70252 
               
               
                   
                 DU145_9 
                 14.30111 
                 9117.509 
                 1654.529 
                 5.500428 
               
               
                   
                 PANC10_18 
                 24.65567 
                 8032.818 
                 2118.744 
                 31.23052 
               
               
                   
                 LNCaP_7 
                 7.319434 
                 7679.132 
                 725.6696 
                 2.091267 
               
               
                   
                 22RV1_18 
                 20.5442 
                 5183.559 
                 493.0609 
                 11.55611 
               
               
                   
                 PC3_18 
                 74.4259 
                 13456.2 
                 2266.607 
                 43.97894 
               
               
                   
                 DU145_18 
                 12.65929 
                 7606.427 
                 939.5005 
                 4.521177 
               
               
                   
                 PANC10_27 
                 15.42979 
                 8119.496 
                 2350.471 
                 24.00189 
               
               
                   
                 LNCaP_16 
                 14.90222 
                 7503.267 
                 909.0353 
                 1.241851 
               
               
                   
                 22RV1_27 
                 31.39789 
                 5252.563 
                 513.5074 
                 17.2182 
               
               
                   
                 PC3_27 
                 38.71292 
                 10959.04 
                 728.3278 
                 22.30914 
               
               
                   
                 DU145_27 
                 0 
                 7858.821 
                 1106.876 
                 0 
               
               
                   
                 PC3_PSI_1 
                 136.1339 
                 11451.22 
                 8597.273 
                 67.37239 
               
               
                   
                 PC3_PSI_2 
                 101.8413 
                 12176.28 
                 8531.675 
                 62.41887 
               
               
                   
                 PC3_PSI_3 
                 99.49826 
                 10847.7 
                 8207.413 
                 55.71903 
               
               
                   
                 PANC10_4 
                 20.43812 
                 6899 
                 9683.125 
                 10.21906 
               
               
                   
                 PC3_2 
                 74.74961 
                 13316.53 
                 1232.785 
                 29.19907 
               
               
                   
                 DU145_2 
                 22.15643 
                 7367.014 
                 1418.012 
                 5.539108 
               
               
                   
                 PANC10_11 
                 27.54277 
                 7995.665 
                 2921.369 
                 19.27994 
               
               
                   
                 LNCaP_2 
                 28.13912 
                 11285.19 
                 1315.504 
                 5.627824 
               
               
                   
                 22RV1_11 
                 5.725772 
                 5800.923 
                 302.7502 
                 15.03015 
               
               
                   
                 DU145_11 
                 7.052371 
                 13331.33 
                 1395.194 
                 4.701581 
               
               
                   
                 PANC10_19 
                 21.35223 
                 8454.15 
                 2243.319 
                 16.01418 
               
               
                   
                 PANC10_20 
                 14.75741 
                 8604.187 
                 2191.476 
                 24.59569 
               
               
                   
                 LNCaP_11 
                 25.19661 
                 8225.953 
                 867.0599 
                 13.33938 
               
               
                   
                 LNCaP_12 
                 10.45179 
                 8051.363 
                 918.0156 
                 5.225895 
               
               
                   
                 22RV1_19 
                 20.55832 
                 5171.702 
                 395.7476 
                 25.6979 
               
               
                   
                 PC3_19 
                 50.17912 
                 10696.63 
                 830.4291 
                 22.26257 
               
               
                   
                 PC3_22 
                 43.0807 
                 11520.29 
                 807.7632 
                 21.54035 
               
               
                   
                 DU145_19 
                 7.264232 
                 6830.453 
                 906.9912 
                 0 
               
               
                   
                 DU145_20 
                 13.84383 
                 6998.054 
                 810.8527 
                 4.944224 
               
               
                   
                 LNCaP_23 
                 12.07445 
                 7374.039 
                 721.0171 
                 5.174764 
               
               
                   
                 22RV1_5 
                 11.40246 
                 5530.191 
                 318.3916 
                 7.016895 
               
               
                   
                 PC3_5 
                 63.17937 
                 13791.59 
                 1123.36 
                 30.04872 
               
               
                   
                 DU145_5 
                 22.94728 
                 6909.127 
                 2174.005 
                 21.94957 
               
               
                   
                 PANC10_14 
                 26.05403 
                 8333.929 
                 2506.23 
                 17.64951 
               
               
                   
                 LNCaP_5 
                 10.46879 
                 8439.587 
                 819.1825 
                 2.617197 
               
               
                   
                 22RV1_14 
                 12.81486 
                 5604.364 
                 309.2652 
                 11.96053 
               
               
                   
                 PC3_14 
                 132.6007 
                 19719.53 
                 3896.468 
                 67.85669 
               
               
                   
                 DU145_14 
                 21.5419 
                 12404.54 
                 3389.258 
                 13.16449 
               
               
                   
                 PANC10_22 
                 21.661 
                 7066.846 
                 1907.062 
                 21.88431 
               
               
                   
                 PANC10_23 
                 12.50035 
                 7958.916 
                 1831.572 
                 10.32637 
               
               
                   
                 LNCaP_14 
                 9.555543 
                 8537.347 
                 745.3323 
                 2.123454 
               
               
                   
                 LNCaP_15 
                 8.461708 
                 9301.835 
                 704.7394 
                 3.626446 
               
               
                   
                 22RV1_22 
                 13.79367 
                 5359.467 
                 392.4926 
                 21.31749 
               
               
                   
                 22RV1_23 
                 14.04224 
                 5195.629 
                 359.2473 
                 12.87205 
               
               
                   
                 PC3_20 
                 81.87521 
                 13993.34 
                 1294.028 
                 43.26739 
               
               
                   
                 PC3_23 
                 81.47088 
                 13702.72 
                 1202.549 
                 34.63732 
               
               
                   
                 DU145_22 
                 12.95103 
                 5573.652 
                 898.3305 
                 1.177366 
               
               
                   
                 DU145_23 
                 6.193339 
                 5824.393 
                 844.9484 
                 1.769525 
               
               
                   
                 PANC10_6 
                 20.99858 
                 7886.647 
                 1809.238 
                 29.39801 
               
               
                   
                 LNCaP_24 
                 7.859865 
                 7931.914 
                 858.0353 
                 11.7898 
               
               
                   
                 22RV1_8 
                 13.66185 
                 6635.862 
                 321.8124 
                 9.107898 
               
               
                   
                 PC3_8 
                 135.5495 
                 15911.42 
                 2852.153 
                 60.95715 
               
               
                   
                 DU145_8 
                 13.92246 
                 7322.221 
                 1228.16 
                 6.961232 
               
               
                   
                 PANC10_17 
                 10.20807 
                 7970.231 
                 1772.234 
                 30.6242 
               
               
                   
                 LNCaP_8 
                 21.67482 
                 8740.373 
                 957.7563 
                 4.064029 
               
               
                   
                 22RV1_17 
                 14.54673 
                 5682.482 
                 416.5654 
                 10.57944 
               
               
                   
                 PC3_17 
                 117.9531 
                 15416.19 
                 2191.599 
                 45.87067 
               
               
                   
                 DU145_17 
                 12.08409 
                 7421.831 
                 1130.412 
                 5.49277 
               
               
                   
                 PANC10_26 
                 7.498328 
                 8108.327 
                 1471.699 
                 14.794 
               
               
                   
                 LNCaP_17 
                 10.15233 
                 7925.162 
                 719.5464 
                 3.807124 
               
               
                   
                 LNCaP_18 
                 13.54721 
                 7599.983 
                 600.9154 
                 6.773603 
               
               
                   
                 22RV1_25 
                 22.18001 
                 5497.229 
                 406.0647 
                 15.35539 
               
               
                   
                 22RV1_26 
                 20.86613 
                 5348.149 
                 378.8005 
                 16.05087 
               
               
                   
                 PC3_21 
                 47.88369 
                 11973.88 
                 763.1771 
                 24.18867 
               
               
                   
                 PC3_24 
                 60.04415 
                 12247.05 
                 845.4998 
                 28.80167 
               
               
                   
                 DU145_25 
                 7.364225 
                 6414.625 
                 902.9969 
                 3.792026 
               
               
                   
                 DU145_26 
                 0 
                 4582.445 
                 1205.906 
                 0 
               
               
                   
                 PC3_PSI_4 
                 116.0458 
                 10641.75 
                 6466.968 
                 55.15048 
               
               
                   
                 PC3_PSI_7 
                 65.41332 
                 11098.76 
                 5544.003 
                 27.77826 
               
               
                   
                 PC3_PSI_10 
                 69.31666 
                 12269.51 
                 4685.072 
                 45.90507 
               
               
                   
                 PC3_PSI_5 
                 109.853 
                 10928.2 
                 7134.154 
                 59.52388 
               
               
                   
                 PC3_PSI_8 
                 65.67656 
                 11224.48 
                 5054.433 
                 49.70118 
               
               
                   
                 PC3_PSI_11 
                 75.28531 
                 11772.94 
                 4058.599 
                 29.63358 
               
               
                   
                 PC3_PSI_6 
                 138.6389 
                 11600.86 
                 6114.117 
                 60.21691 
               
               
                   
                 PC3_PSI_9 
                 72.50337 
                 10328.86 
                 5277.098 
                 31.81803 
               
               
                   
                 PC3_PSI_12 
                 64.40419 
                 10479.2 
                 6131.746 
                 27.02974 
               
               
                   
                 PANC10_1 
                 104.4371 
                 9649.989 
                 16746.49 
                 36.55299 
               
               
                   
                 LNCaP_19 
                 167.5489 
                 25812.72 
                 4163.954 
                 37.88061 
               
               
                   
                 22RV1_1 
                 14.30558 
                 5440.413 
                 343.334 
                 17.1667 
               
               
                   
                 PC3_1 
                 228.2603 
                 19178.65 
                 3507.706 
                 102.1584 
               
               
                   
                 DU145_1 
                 134.8219 
                 11298.74 
                 4204.691 
                 44.94065 
               
               
                   
                 PANC10_10 
                 249.9687 
                 14071.9 
                 14980.26 
                 214.2588 
               
               
                   
                 LNCaP_3 
                 65.78962 
                 19872.12 
                 3352.834 
                 45.07807 
               
               
                   
                 22RV1_10 
                 24.73002 
                 10677.75 
                 1065.639 
                 31.47457 
               
               
                   
                 DU145_10 
                 103.5395 
                 15795.34 
                 4245.976 
                 51.34191 
               
               
                   
                 PANC10_2 
                 311.985 
                 30121.65 
                 32468.58 
                 255.6264 
               
               
                   
                 22RV1_4 
                 37.59235 
                 12027.88 
                 614.0085 
                 33.41543 
               
               
                   
                 PC3_4 
                 642.7761 
                 54808.26 
                 21785.28 
                 307.6616 
               
               
                   
                 DU145_4 
                 197.3824 
                 18925.56 
                 10973 
                 73.1046 
               
               
                   
                 PANC10_13 
                 191.7071 
                 29308.47 
                 24094.22 
                 162.633 
               
               
                   
                 LNCaP_6 
                 65.2604 
                 20293.57 
                 3592.948 
                 26.58757 
               
               
                   
                 22RV1_13 
                 34.5536 
                 9575.536 
                 962.2655 
                 33.50652 
               
               
                   
                 PC3_13 
                 699.7048 
                 56612.26 
                 37270.24 
                 392.4105 
               
               
                   
                 DU145_13 
                 93.62045 
                 27383.16 
                 14038.14 
                 70.62596 
               
               
                   
                 PANC10_3 
                 204.9732 
                 30376.43 
                 24958.85 
                 187.0193 
               
               
                   
                 LNCaP_21 
                 163.7853 
                 26070.1 
                 3826.363 
                 14.11942 
               
               
                   
                 22RV1_7 
                 47.28626 
                 16204.64 
                 1003.924 
                 42.7395 
               
               
                   
                 PC3_7 
                 636.1495 
                 71921.8 
                 46350.09 
                 299.622 
               
               
                   
                 DU145_7 
                 91.43509 
                 15639.6 
                 9443.038 
                 52.5489 
               
               
                   
                 PANC10_16 
                 173.3544 
                 26166.94 
                 30138.14 
                 189.3073 
               
               
                   
                 LNCaP_9 
                 73.05553 
                 19577.76 
                 3049.225 
                 32.59401 
               
               
                   
                 22RV1_16 
                 23.63488 
                 11998.41 
                 1079.101 
                 41.19222 
               
               
                   
                 PC3_16 
                 550.2457 
                 61562.82 
                 36378.06 
                 275.4092 
               
               
                   
                 DU145_16 
                 34.21911 
                 17995.65 
                 11369.75 
                 36.02011 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 Oxygen-Sensitive Candidates 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 O2 
                 PSI 
                 Expt 
                 CellLine 
                 BNIP3 
                 PPP1R3G 
                 KDM3A 
                 BHLHE40 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 LNCaP_25 
                 20 
                 0 
                 A 
                 LNCaP 
                 1884.493 
                 0 
                 336.6374 
                 201.3058 
               
               
                 22RV1_3 
                 20 
                 0 
                 A 
                 22RV1 
                 377.7168 
                 1.339421 
                 310.7458 
                 2.678843 
               
               
                 PC3_3 
                 20 
                 0 
                 A 
                 PC3 
                 535.9478 
                 26.82655 
                 506.7885 
                 2151.956 
               
               
                 DU145_3 
                 20 
                 0 
                 A 
                 DU145 
                 1537.72 
                 43.74737 
                 534.8115 
                 313.8873 
               
               
                 PANC10_12 
                 20 
                 0 
                 B 
                 PANC10 
                 6.94829 
                 13.89658 
                 821.2879 
                 187.6038 
               
               
                 LNCaP_1 
                 20 
                 0 
                 B 
                 LNCaP 
                 2310.132 
                 1.1352 
                 353.0473 
                 188.4432 
               
               
                 22RV1_12 
                 20 
                 0 
                 B 
                 22RV1 
                 455.3357 
                 1.782136 
                 336.8237 
                 5.346407 
               
               
                 DU145_12 
                 20 
                 0 
                 B 
                 DU145 
                 2355.062 
                 58.90487 
                 412.3341 
                 404.4046 
               
               
                 PANC10_21 
                 20 
                 0 
                 C 
                 PANC10 
                 4.41638 
                 14.57405 
                 1080.247 
                 197.4122 
               
               
                 LNCaP_10 
                 20 
                 0 
                 C 
                 LNCaP 
                 2106.632 
                 0 
                 274.8215 
                 273.8222 
               
               
                 22RV1_21 
                 20 
                 0 
                 C 
                 22RV1 
                 384.4058 
                 2.351106 
                 355.0169 
                 8.22887 
               
               
                 PC3_25 
                 20 
                 0 
                 C 
                 PC3 
                 473.4583 
                 21.48327 
                 481.7211 
                 1764.933 
               
               
                 DU145_21 
                 20 
                 0 
                 C 
                 DU145 
                 1772.991 
                 56.54185 
                 465.4606 
                 379.6382 
               
               
                 PANC10_8 
                 20 
                 0 
                 A 
                 PANC10 
                 12.12097 
                 7.272581 
                 898.7699 
                 173.3299 
               
               
                 LNCaP_26 
                 20 
                 0 
                 A 
                 LNCaP 
                 1737.064 
                 0 
                 346.6829 
                 209.8344 
               
               
                 22RV1_6 
                 20 
                 0 
                 A 
                 22RV1 
                 375.8526 
                 0 
                 370.718 
                 1.02692 
               
               
                 PC3_6 
                 20 
                 0 
                 A 
                 PC3 
                 652.4015 
                 31.44985 
                 544.1555 
                 1584.195 
               
               
                 DU145_6 
                 20 
                 0 
                 A 
                 DU145 
                 1697.921 
                 55.25438 
                 534.1256 
                 370.6648 
               
               
                 PANC10_15 
                 20 
                 0 
                 B 
                 PANC10 
                 11.38174 
                 3.414522 
                 1059.64 
                 175.2788 
               
               
                 LNCaP_4 
                 20 
                 0 
                 B 
                 LNCaP 
                 2423.8 
                 0 
                 315.4445 
                 227.821 
               
               
                 22RV1_15 
                 20 
                 0 
                 B 
                 22RV1 
                 447.4213 
                 0.974774 
                 380.1618 
                 5.848644 
               
               
                 PC3_15 
                 20 
                 0 
                 B 
                 PC3 
                 859.6456 
                 43.93141 
                 709.411 
                 1654.75 
               
               
                 DU145_15 
                 20 
                 0 
                 B 
                 DU145 
                 2327.797 
                 101.8222 
                 502.054 
                 634.1204 
               
               
                 PANC10_24 
                 20 
                 0 
                 C 
                 PANC10 
                 9.549055 
                 6.820754 
                 841.681 
                 201.4396 
               
               
                 LNCaP_13 
                 20 
                 0 
                 C 
                 LNCaP 
                 2220.541 
                 0 
                 262.4169 
                 267.1239 
               
               
                 22RV1_24 
                 20 
                 0 
                 C 
                 22RV1 
                 497.1909 
                 4.220636 
                 406.8693 
                 11.81778 
               
               
                 PC3_26 
                 20 
                 0 
                 C 
                 PC3 
                 486.6086 
                 29.25811 
                 480.449 
                 1790.905 
               
               
                 DU145_24 
                 20 
                 0 
                 C 
                 DU145 
                 1195.662 
                 46.98399 
                 460.1191 
                 272.1831 
               
               
                 PANC10_9 
                 20 
                 0 
                 A 
                 PANC10 
                 9.740092 
                 6.493395 
                 986.996 
                 181.8151 
               
               
                 LNCaP_27 
                 20 
                 0 
                 A 
                 LNCaP 
                 1826.249 
                 0 
                 368.8781 
                 288.249 
               
               
                 22RV1_9 
                 20 
                 0 
                 A 
                 22RV1 
                 418.7484 
                 2.052688 
                 336.6409 
                 2.052688 
               
               
                 PC3_9 
                 20 
                 0 
                 A 
                 PC3 
                 882.2434 
                 50.94172 
                 802.1921 
                 2231.359 
               
               
                 DU145_9 
                 20 
                 0 
                 A 
                 DU145 
                 3016.435 
                 74.80582 
                 741.4577 
                 861.367 
               
               
                 PANC10_18 
                 20 
                 0 
                 B 
                 PANC10 
                 8.218557 
                 8.218557 
                 1145.667 
                 261.3501 
               
               
                 LNCaP_7 
                 20 
                 0 
                 B 
                 LNCaP 
                 2469.786 
                 2.091267 
                 397.3407 
                 248.8608 
               
               
                 22RV1_18 
                 20 
                 0 
                 B 
                 22RV1 
                 360.8076 
                 0 
                 382.6358 
                 1.284013 
               
               
                 PC3_18 
                 20 
                 0 
                 B 
                 PC3 
                 726.6674 
                 42.62574 
                 606.9094 
                 1504.756 
               
               
                 DU145_18 
                 20 
                 0 
                 B 
                 DU145 
                 1682.782 
                 56.06259 
                 432.2245 
                 348.1306 
               
               
                 PANC10_27 
                 20 
                 0 
                 C 
                 PANC10 
                 3.428841 
                 15.42979 
                 917.215 
                 231.4468 
               
               
                 LNCaP_16 
                 20 
                 0 
                 C 
                 LNCaP 
                 2055.264 
                 0 
                 289.3514 
                 295.5606 
               
               
                 22RV1_27 
                 20 
                 0 
                 C 
                 22RV1 
                 369.6848 
                 3.038506 
                 383.8645 
                 10.12835 
               
               
                 PC3_27 
                 20 
                 0 
                 C 
                 PC3 
                 432.4036 
                 14.43532 
                 459.9619 
                 1728.958 
               
               
                 DU145_27 
                 20 
                 0 
                 C 
                 DU145 
                 996.1885 
                 0 
                 0 
                 110.6876 
               
               
                 PC3_PSI_1 
                 20 
                 0 
                 A 
                 PC3 
                 1212.009 
                 49.31381 
                 1193.255 
                 2956.051 
               
               
                 PC3_PSI_2 
                 20 
                 0 
                 B 
                 PC3 
                 1408.695 
                 53.87734 
                 1015.128 
                 3076.922 
               
               
                 PC3_PSI_3 
                 20 
                 0 
                 C 
                 PC3 
                 1089.705 
                 60.49494 
                 1467.798 
                 3081.262 
               
               
                 PANC10_4 
                 10 
                 2 
                 A 
                 PANC10 
                 6.812705 
                 44.28258 
                 1556.703 
                 436.0131 
               
               
                 PC3_2 
                 10 
                 2 
                 A 
                 PC3 
                 756.8398 
                 47.30249 
                 706.0334 
                 1985.537 
               
               
                 DU145_2 
                 10 
                 2 
                 A 
                 DU145 
                 1657.301 
                 74.22405 
                 546.156 
                 310.19 
               
               
                 PANC10_11 
                 10 
                 5 
                 B 
                 PANC10 
                 7.344738 
                 8.26283 
                 893.3037 
                 181.7823 
               
               
                 LNCaP_2 
                 10 
                 5 
                 B 
                 LNCaP 
                 3307.754 
                 0 
                 434.7494 
                 406.6103 
               
               
                 22RV1_11 
                 10 
                 5 
                 B 
                 22RV1 
                 422.9914 
                 2.862886 
                 388.6368 
                 5.010051 
               
               
                 DU145_11 
                 10 
                 5 
                 B 
                 DU145 
                 2160.376 
                 45.84041 
                 443.124 
                 266.8147 
               
               
                 PANC10_19 
                 20 
                 2 
                 C 
                 PANC10 
                 6.672573 
                 14.67966 
                 898.1284 
                 218.8604 
               
               
                 PANC10_20 
                 20 
                 5 
                 C 
                 PANC10 
                 6.763815 
                 15.9872 
                 863.3087 
                 270.5526 
               
               
                 LNCaP_11 
                 20 
                 2 
                 C 
                 LNCaP 
                 2048.336 
                 1.482154 
                 385.3599 
                 183.7871 
               
               
                 LNCaP_12 
                 20 
                 5 
                 C 
                 LNCaP 
                 1867.387 
                 1.741965 
                 341.4252 
                 209.0358 
               
               
                 22RV1_19 
                 20 
                 2 
                 C 
                 22RV1 
                 388.0382 
                 6.424474 
                 489.5449 
                 3.854685 
               
               
                 PC3_19 
                 20 
                 5 
                 C 
                 PC3 
                 448.7851 
                 26.50305 
                 519.8133 
                 1988.082 
               
               
                 PC3_22 
                 20 
                 2 
                 C 
                 PC3 
                 454.248 
                 28.50929 
                 527.1051 
                 2062.805 
               
               
                 DU145_19 
                 20 
                 2 
                 C 
                 DU145 
                 1517.187 
                 52.92512 
                 459.7221 
                 214.8137 
               
               
                 DU145_20 
                 20 
                 5 
                 C 
                 DU145 
                 1555.453 
                 59.33068 
                 324.3411 
                 163.1594 
               
               
                 LNCaP_23 
                 10 
                 2 
                 A 
                 LNCaP 
                 1740.446 
                 0 
                 395.007 
                 222.5149 
               
               
                 22RV1_5 
                 10 
                 2 
                 A 
                 22RV1 
                 405.2257 
                 3.508448 
                 440.3102 
                 0.877112 
               
               
                 PC3_5 
                 10 
                 2 
                 A 
                 PC3 
                 759.6934 
                 52.39265 
                 621.007 
                 1768.252 
               
               
                 DU145_5 
                 10 
                 2 
                 A 
                 DU145 
                 2189.969 
                 97.77537 
                 631.5491 
                 451.9617 
               
               
                 PANC10_14 
                 10 
                 5 
                 B 
                 PANC10 
                 12.60679 
                 15.9686 
                 1015.267 
                 196.6659 
               
               
                 LNCaP_5 
                 10 
                 5 
                 B 
                 LNCaP 
                 2971.391 
                 2.617197 
                 423.1135 
                 247.7613 
               
               
                 22RV1_14 
                 10 
                 5 
                 B 
                 22RV1 
                 448.52 
                 3.417295 
                 387.0087 
                 2.562971 
               
               
                 PC3_14 
                 10 
                 5 
                 B 
                 PC3 
                 1152.941 
                 83.42015 
                 827.3535 
                 1848.939 
               
               
                 DU145_14 
                 10 
                 5 
                 B 
                 DU145 
                 2995.52 
                 122.0707 
                 499.0539 
                 581.6312 
               
               
                 PANC10_22 
                 20 
                 2 
                 C 
                 PANC10 
                 8.039135 
                 13.84518 
                 803.5786 
                 181.3272 
               
               
                 PANC10_23 
                 20 
                 5 
                 C 
                 PANC10 
                 12.50035 
                 11.41336 
                 705.9978 
                 197.8316 
               
               
                 LNCaP_14 
                 20 
                 2 
                 C 
                 LNCaP 
                 2168.046 
                 0 
                 295.1601 
                 237.8268 
               
               
                 LNCaP_15 
                 20 
                 5 
                 C 
                 LNCaP 
                 2407.96 
                 0 
                 291.3245 
                 273.1923 
               
               
                 22RV1_22 
                 20 
                 2 
                 C 
                 22RV1 
                 427.6037 
                 2.50794 
                 408.7942 
                 0 
               
               
                 22RV1_23 
                 20 
                 5 
                 C 
                 22RV1 
                 399.0337 
                 0 
                 342.8647 
                 7.02112 
               
               
                 PC3_20 
                 20 
                 5 
                 C 
                 PC3 
                 667.6491 
                 40.60478 
                 574.4578 
                 1722.042 
               
               
                 PC3_23 
                 20 
                 2 
                 C 
                 PC3 
                 632.2531 
                 49.76066 
                 530.2925 
                 1614.782 
               
               
                 DU145_22 
                 20 
                 2 
                 C 
                 DU145 
                 1321.005 
                 34.14363 
                 492.1391 
                 250.779 
               
               
                 DU145_23 
                 20 
                 5 
                 C 
                 DU145 
                 1298.832 
                 57.50957 
                 452.1137 
                 222.0754 
               
               
                 PANC10_6 
                 10 
                 2 
                 A 
                 PANC10 
                 15.11898 
                 16.79887 
                 1069.248 
                 212.9256 
               
               
                 LNCaP_24 
                 10 
                 2 
                 A 
                 LNCaP 
                 2202.072 
                 1.309978 
                 421.8128 
                 273.7853 
               
               
                 22RV1_8 
                 10 
                 2 
                 A 
                 22RV1 
                 425.7942 
                 1.517983 
                 412.8914 
                 3.035966 
               
               
                 PC3_8 
                 10 
                 2 
                 A 
                 PC3 
                 1170.217 
                 52.93647 
                 982.533 
                 2756.707 
               
               
                 DU145_8 
                 10 
                 2 
                 A 
                 DU145 
                 1988.923 
                 83.53478 
                 681.2063 
                 594.6881 
               
               
                 PANC10_17 
                 10 
                 5 
                 B 
                 PANC10 
                 5.104033 
                 15.3121 
                 1093.397 
                 250.0976 
               
               
                 LNCaP_8 
                 10 
                 5 
                 B 
                 LNCaP 
                 2407.26 
                 0 
                 306.1569 
                 268.2259 
               
               
                 22RV1_17 
                 10 
                 5 
                 B 
                 22RV1 
                 427.1449 
                 2.64486 
                 407.3084 
                 3.96729 
               
               
                 PC3_17 
                 10 
                 5 
                 B 
                 PC3 
                 858.4368 
                 53.15173 
                 736.115 
                 1716.145 
               
               
                 DU145_17 
                 10 
                 5 
                 B 
                 DU145 
                 1856.556 
                 97.77131 
                 515.2219 
                 582.2337 
               
               
                 PANC10_26 
                 20 
                 5 
                 C 
                 PANC10 
                 7.498328 
                 11.75414 
                 959.3807 
                 143.6842 
               
               
                 LNCaP_17 
                 20 
                 2 
                 C 
                 LNCaP 
                 2044.425 
                 1.269041 
                 280.4581 
                 291.8795 
               
               
                 LNCaP_18 
                 20 
                 5 
                 C 
                 LNCaP 
                 2672.67 
                 0 
                 345.4538 
                 241.9144 
               
               
                 22RV1_25 
                 20 
                 2 
                 C 
                 22RV1 
                 390.7094 
                 3.412309 
                 412.8894 
                 3.412309 
               
               
                 22RV1_26 
                 20 
                 5 
                 C 
                 22RV1 
                 420.5327 
                 1.605087 
                 418.9277 
                 3.210174 
               
               
                 PC3_21 
                 20 
                 5 
                 C 
                 PC3 
                 500.5573 
                 35.04888 
                 484.267 
                 1771.696 
               
               
                 PC3_24 
                 20 
                 2 
                 C 
                 PC3 
                 491.581 
                 24.89636 
                 471.0781 
                 1757.878 
               
               
                 DU145_25 
                 20 
                 2 
                 C 
                 DU145 
                 1469.877 
                 60.67242 
                 375.8503 
                 206.1983 
               
               
                 DU145_26 
                 20 
                 5 
                 C 
                 DU145 
                 964.7252 
                 0 
                 241.1813 
                 0 
               
               
                 PC3_PSI_4 
                 20 
                 0.5 
                 A 
                 PC3 
                 1100.137 
                 90.7685 
                 1469.531 
                 3095.321 
               
               
                 PC3_PSI_7 
                 20 
                 2 
                 A 
                 PC3 
                 918.0267 
                 43.90757 
                 1006.738 
                 2725.854 
               
               
                 PC3_PSI_10 
                 20 
                 5 
                 A 
                 PC3 
                 907.5433 
                 41.77361 
                 820.3236 
                 2921.858 
               
               
                 PC3_PSI_5 
                 20 
                 0.5 
                 B 
                 PC3 
                 1087.4 
                 70.17042 
                 1380.18 
                 3091.854 
               
               
                 PC3_PSI_8 
                 20 
                 2 
                 B 
                 PC3 
                 913.2592 
                 46.1511 
                 1042.837 
                 2678.539 
               
               
                 PC3_PSI_11 
                 20 
                 5 
                 B 
                 PC3 
                 872.1883 
                 46.45264 
                 863.7788 
                 3215.243 
               
               
                 PC3_PSI_6 
                 20 
                 0.5 
                 C 
                 PC3 
                 1179.131 
                 120.4338 
                 1625.856 
                 2820.392 
               
               
                 PC3_PSI_9 
                 20 
                 2 
                 C 
                 PC3 
                 888.8183 
                 68.85212 
                 973.8403 
                 2787.989 
               
               
                 PC3_PSI_12 
                 20 
                 5 
                 C 
                 PC3 
                 916.3415 
                 54.72688 
                 1090.533 
                 2917.877 
               
               
                 PANC10_1 
                 1 
                 2 
                 A 
                 PANC10 
                 10.44371 
                 130.5464 
                 2637.037 
                 877.2717 
               
               
                 LNCaP_19 
                 1 
                 2 
                 A 
                 LNCaP 
                 11168.95 
                 2.913893 
                 1041.717 
                 926.6181 
               
               
                 22RV1_1 
                 1 
                 2 
                 A 
                 22RV1 
                 467.7926 
                 1.430558 
                 702.4041 
                 0 
               
               
                 PC3_1 
                 1 
                 2 
                 A 
                 PC3 
                 1877.161 
                 114.1301 
                 1523.597 
                 3456.626 
               
               
                 DU145_1 
                 1 
                 2 
                 A 
                 DU145 
                 4441.451 
                 204.9732 
                 1043.5 
                 1381.103 
               
               
                 PANC10_10 
                 1 
                 5 
                 B 
                 PANC10 
                 10.04338 
                 92.62231 
                 2322.253 
                 790.0795 
               
               
                 LNCaP_3 
                 1 
                 5 
                 B 
                 LNCaP 
                 9018.051 
                 6.091631 
                 877.1949 
                 565.3034 
               
               
                 22RV1_10 
                 1 
                 5 
                 B 
                 22RV1 
                 2023.365 
                 16.86138 
                 919.5071 
                 21.35775 
               
               
                 DU145_10 
                 1 
                 5 
                 B 
                 DU145 
                 4712.332 
                 162.5827 
                 842.8631 
                 1135.512 
               
               
                 PANC10_2 
                 1 
                 2 
                 A 
                 PANC10 
                 11.57364 
                 82.02187 
                 3135.449 
                 946.5223 
               
               
                 22RV1_4 
                 1 
                 2 
                 A 
                 22RV1 
                 1756.816 
                 22.55541 
                 1064.281 
                 11.6954 
               
               
                 PC3_4 
                 1 
                 2 
                 A 
                 PC3 
                 5423.365 
                 169.4506 
                 1770.238 
                 5201.848 
               
               
                 DU145_4 
                 1 
                 2 
                 A 
                 DU145 
                 4238.848 
                 313.1314 
                 1129.466 
                 1706.992 
               
               
                 PANC10_13 
                 1 
                 5 
                 B 
                 PANC10 
                 6.359951 
                 131.7418 
                 2111.504 
                 914.9243 
               
               
                 LNCaP_6 
                 1 
                 5 
                 B 
                 LNCaP 
                 8195.015 
                 6.04263 
                 861.679 
                 576.4669 
               
               
                 22RV1_13 
                 1 
                 5 
                 B 
                 22RV1 
                 1859.612 
                 20.94158 
                 894.2053 
                 32.45944 
               
               
                 PC3_13 
                 1 
                 5 
                 B 
                 PC3 
                 4191.345 
                 268.4914 
                 2037.781 
                 5547.57 
               
               
                 DU145_13 
                 1 
                 5 
                 B 
                 DU145 
                 5865.239 
                 364.627 
                 652.0583 
                 1364.888 
               
               
                 PANC10_3 
                 1 
                 2 
                 A 
                 PANC10 
                 5.984618 
                 133.1578 
                 2558.424 
                 951.5543 
               
               
                 LNCaP_21 
                 1 
                 2 
                 A 
                 LNCaP 
                 8482.947 
                 2.823884 
                 1042.013 
                 883.8756 
               
               
                 22RV1_7 
                 1 
                 2 
                 A 
                 22RV1 
                 3168.179 
                 34.55534 
                 1164.879 
                 24.55248 
               
               
                 PC3_7 
                 1 
                 2 
                 A 
                 PC3 
                 5787.271 
                 200.7905 
                 2149.898 
                 6611.701 
               
               
                 DU145_7 
                 1 
                 2 
                 A 
                 DU145 
                 4169.23 
                 240.674 
                 1067.794 
                 1692.075 
               
               
                 PANC10_16 
                 1 
                 5 
                 B 
                 PANC10 
                 18.07991 
                 142.5122 
                 2173.843 
                 1168.813 
               
               
                 LNCaP_9 
                 1 
                 5 
                 B 
                 LNCaP 
                 7559.562 
                 6.743587 
                 987.9356 
                 560.8417 
               
               
                 22RV1_16 
                 1 
                 5 
                 B 
                 22RV1 
                 1931.307 
                 14.18093 
                 937.2917 
                 31.06298 
               
               
                 PC3_16 
                 1 
                 5 
                 B 
                 PC3 
                 4419.143 
                 254.2238 
                 1826.518 
                 4920.72 
               
               
                 DU145_16 
                 1 
                 5 
                 B 
                 DU145 
                 8228.795 
                 590.7299 
                 304.37 
                 633.954 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Pressure-Sensitive Candidates 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 O2 
                 PSI 
                 Expt 
                 CellLine 
                 RBM3 
                 CBX3P2 
                 TMEM260 
                 TBCK 
                 COX11 
                 FBXO36 
                 ACBD6 
                 EXOSC9 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 LNCaP_25 
                 20 
                 0 
                 A 
                 LNCaP 
                 2341.237 
                 20.29974 
                 228.3721 
                 221.6055 
                 676.6581 
                 42.29113 
                 338.329 
                 605.609 
               
               
                 22RV1_3 
                 20 
                 0 
                 A 
                 22RV1 
                 3323.104 
                 4.018264 
                 225.0228 
                 117.8691 
                 409.8629 
                 121.8873 
                 543.8051 
                 692.4809 
               
               
                 PC3_3 
                 20 
                 0 
                 A 
                 PC3 
                 4555.265 
                 7.581416 
                 397.1495 
                 82.2292 
                 964.0062 
                 51.32035 
                 285.7611 
                 415.8115 
               
               
                 DU145_3 
                 20 
                 0 
                 A 
                 DU145 
                 4080.535 
                 15.31158 
                 320.4494 
                 79.83894 
                 926.3505 
                 29.52947 
                 530.4368 
                 692.3021 
               
               
                 PANC10_8 
                 20 
                 0 
                 A 
                 PANC10 
                 6270.177 
                 0.606048 
                 235.7528 
                 93.33146 
                 307.2666 
                 54.54436 
                 1032.101 
                 522.4138 
               
               
                 LNCaP_26 
                 20 
                 0 
                 A 
                 LNCaP 
                 2751.567 
                 25.54505 
                 242.678 
                 215.3083 
                 824.7403 
                 58.3887 
                 326.6118 
                 571.1144 
               
               
                 22RV1_6 
                 20 
                 0 
                 A 
                 22RV1 
                 2628.915 
                 6.161519 
                 216.6801 
                 138.6342 
                 417.9564 
                 137.6073 
                 546.3213 
                 668.5248 
               
               
                 PC3_6 
                 20 
                 0 
                 A 
                 PC3 
                 4268.403 
                 5.851134 
                 368.6215 
                 136.7703 
                 1071.489 
                 65.09387 
                 277.9289 
                 398.6085 
               
               
                 DU145_6 
                 20 
                 0 
                 A 
                 DU145 
                 4347.829 
                 12.66246 
                 279.7253 
                 90.93949 
                 920.9063 
                 18.41813 
                 569.8107 
                 626.2163 
               
               
                 PANC10_9 
                 20 
                 0 
                 A 
                 PANC10 
                 5259.65 
                 0 
                 249.9957 
                 126.6212 
                 288.9561 
                 25.97358 
                 922.0621 
                 461.031 
               
               
                 LNCaP_27 
                 20 
                 0 
                 A 
                 LNCaP 
                 2539.816 
                 34.26736 
                 255.9974 
                 203.5885 
                 691.3944 
                 28.22018 
                 336.6264 
                 554.325 
               
               
                 22RV1_9 
                 20 
                 0 
                 A 
                 22RV1 
                 2625.388 
                 6.158065 
                 221.6903 
                 131.3721 
                 359.2205 
                 143.6882 
                 582.9635 
                 689.7033 
               
               
                 PC3_9 
                 20 
                 0 
                 A 
                 PC3 
                 3764.649 
                 4.478393 
                 378.4242 
                 124.2754 
                 1153.746 
                 75.01308 
                 274.8614 
                 372.8262 
               
               
                 DU145_9 
                 20 
                 0 
                 A 
                 DU145 
                 3828.298 
                 1.100086 
                 361.9281 
                 116.6091 
                 1103.386 
                 19.80154 
                 561.0436 
                 597.3464 
               
               
                 PC3_PSI_1 
                 20 
                 0 
                 A 
                 PC3 
                 3345.699 
                 18.75314 
                 585.5147 
                 181.9749 
                 1030.728 
                 113.908 
                 508.4185 
                 203.5063 
               
               
                 PANC10_12 
                 20 
                 0 
                 B 
                 PANC10 
                 6561.966 
                 4.168974 
                 244.5798 
                 75.04154 
                 286.2696 
                 66.70359 
                 926.9019 
                 397.4422 
               
               
                 LNCaP_1 
                 20 
                 0 
                 B 
                 LNCaP 
                 2565.552 
                 24.9744 
                 296.2872 
                 228.1752 
                 751.5025 
                 44.27281 
                 333.7488 
                 643.6585 
               
               
                 22RV1_12 
                 20 
                 0 
                 B 
                 22RV1 
                 2731.123 
                 7.128543 
                 272.6668 
                 145.2441 
                 412.5644 
                 127.4227 
                 539.9871 
                 676.3205 
               
               
                 DU145_12 
                 20 
                 0 
                 B 
                 DU145 
                 6787.653 
                 10.19507 
                 317.18 
                 138.1999 
                 1218.878 
                 44.17865 
                 456.5127 
                 872.2451 
               
               
                 PANC10_15 
                 20 
                 0 
                 B 
                 PANC10 
                 5285.68 
                 5.69087 
                 227.6348 
                 138.8572 
                 293.6489 
                 28.45435 
                 1040.291 
                 452.9933 
               
               
                 LNCaP_4 
                 20 
                 0 
                 B 
                 LNCaP 
                 2600.395 
                 16.17664 
                 203.5561 
                 188.7275 
                 644.3695 
                 52.57408 
                 358.5822 
                 474.5148 
               
               
                 22RV1_15 
                 20 
                 0 
                 B 
                 22RV1 
                 2673.805 
                 3.899096 
                 251.4917 
                 147.1909 
                 423.0519 
                 125.7458 
                 530.277 
                 669.6697 
               
               
                 PC3_15 
                 20 
                 0 
                 B 
                 PC3 
                 2805.644 
                 10.84726 
                 374.7729 
                 129.6248 
                 997.9482 
                 71.59193 
                 258.1648 
                 391.0438 
               
               
                 DU145_15 
                 20 
                 0 
                 B 
                 DU145 
                 4734.228 
                 9.073265 
                 275.2224 
                 104.8466 
                 858.9358 
                 31.25236 
                 499.0296 
                 725.8612 
               
               
                 PANC10_18 
                 20 
                 0 
                 B 
                 PANC10 
                 4834.155 
                 1.643711 
                 259.7064 
                 128.2095 
                 386.2722 
                 75.61073 
                 943.4904 
                 460.2392 
               
               
                 LNCaP_7 
                 20 
                 0 
                 B 
                 LNCaP 
                 3271.787 
                 40.77971 
                 240.4957 
                 174.6208 
                 737.1716 
                 37.64281 
                 388.9757 
                 673.388 
               
               
                 22RV1_18 
                 20 
                 0 
                 B 
                 22RV1 
                 2480.712 
                 0 
                 296.6069 
                 118.1292 
                 403.18 
                 116.8452 
                 463.5286 
                 680.5267 
               
               
                 PC3_18 
                 20 
                 0 
                 B 
                 PC3 
                 3387.732 
                 10.82559 
                 347.0953 
                 169.1498 
                 1035.873 
                 58.18752 
                 288.2312 
                 374.1593 
               
               
                 DU145_18 
                 20 
                 0 
                 B 
                 DU145 
                 3878.265 
                 9.042353 
                 272.1748 
                 134.7311 
                 993.7546 
                 41.59482 
                 646.5282 
                 697.1654 
               
               
                 PC3_PSI_2 
                 20 
                 0 
                 B 
                 PC3 
                 4171.552 
                 19.71122 
                 517.7481 
                 95.27091 
                 949.4239 
                 122.2096 
                 739.8279 
                 224.0509 
               
               
                 PANC10_21 
                 20 
                 0 
                 C 
                 PANC10 
                 6169.241 
                 5.299656 
                 268.0743 
                 135.1412 
                 317.9793 
                 61.82932 
                 1016.651 
                 535.7069 
               
               
                 LNCaP_10 
                 20 
                 0 
                 C 
                 LNCaP 
                 3535.704 
                 18.98767 
                 196.8721 
                 163.8936 
                 670.5645 
                 58.96171 
                 354.7696 
                 599.6106 
               
               
                 22RV1_21 
                 20 
                 0 
                 C 
                 22RV1 
                 3391.47 
                 12.93108 
                 228.0572 
                 124.6086 
                 410.2679 
                 136.3641 
                 557.212 
                 618.3408 
               
               
                 PC3_25 
                 20 
                 0 
                 C 
                 PC3 
                 3779.403 
                 14.04676 
                 329.6856 
                 147.0778 
                 1022.934 
                 69.4075 
                 303.2447 
                 444.5385 
               
               
                 DU145_21 
                 20 
                 0 
                 C 
                 DU145 
                 4444.594 
                 2.019352 
                 268.5738 
                 122.1708 
                 1058.14 
                 33.31931 
                 698.6958 
                 657.2991 
               
               
                 PANC10_24 
                 20 
                 0 
                 C 
                 PANC10 
                 6779.829 
                 1.364151 
                 243.7283 
                 105.949 
                 321.4849 
                 50.92829 
                 866.2357 
                 544.7509 
               
               
                 LNCaP_13 
                 20 
                 0 
                 C 
                 LNCaP 
                 3172.538 
                 24.7119 
                 182.3974 
                 167.0995 
                 728.4128 
                 48.24705 
                 358.911 
                 487.1775 
               
               
                 22RV1_24 
                 20 
                 0 
                 C 
                 22RV1 
                 2659.845 
                 5.064763 
                 244.7969 
                 152.787 
                 405.1811 
                 157.8518 
                 522.5148 
                 608.6157 
               
               
                 PC3_26 
                 20 
                 0 
                 C 
                 PC3 
                 3815.104 
                 10.00935 
                 309.52 
                 130.8916 
                 942.4193 
                 62.36598 
                 322.6092 
                 448.8811 
               
               
                 DU145_24 
                 20 
                 0 
                 C 
                 DU145 
                 3565.923 
                 8.100688 
                 251.1213 
                 132.8513 
                 852.1924 
                 43.74372 
                 665.8766 
                 638.3342 
               
               
                 PANC10_27 
                 20 
                 0 
                 C 
                 PANC10 
                 5134.69 
                 3.428841 
                 183.443 
                 132.0104 
                 294.8803 
                 39.43167 
                 872.6401 
                 375.4581 
               
               
                 LNCaP_16 
                 20 
                 0 
                 C 
                 LNCaP 
                 3575.29 
                 19.86962 
                 194.9707 
                 166.4081 
                 613.4746 
                 45.9485 
                 356.4114 
                 500.4661 
               
               
                 22RV1_27 
                 20 
                 0 
                 C 
                 22RV1 
                 2834.926 
                 5.064176 
                 231.9393 
                 131.6686 
                 408.1726 
                 127.6172 
                 508.4433 
                 645.176 
               
               
                 PC3_27 
                 20 
                 0 
                 C 
                 PC3 
                 3813.55 
                 12.46687 
                 361.5393 
                 154.8517 
                 1049.186 
                 84.6435 
                 299.2049 
                 374.0061 
               
               
                 DU145_27 
                 20 
                 0 
                 C 
                 DU145 
                 2988.566 
                 0 
                 110.6876 
                 442.7505 
                 1217.564 
                 0 
                 885.5009 
                 221.3752 
               
               
                 PC3_PSI_3 
                 20 
                 0 
                 C 
                 PC3 
                 3076.486 
                 13.53176 
                 567.5381 
                 179.8929 
                 992.5947 
                 144.0735 
                 473.6117 
                 220.4881 
               
               
                 PANC10_4 
                 10 
                 2 
                 A 
                 PANC10 
                 6386.911 
                 1.135451 
                 172.5885 
                 70.39795 
                 373.5633 
                 44.28258 
                 1051.428 
                 463.264 
               
               
                 PC3_2 
                 10 
                 2 
                 A 
                 PC3 
                 4264.816 
                 16.93546 
                 345.7169 
                 85.26127 
                 1086.205 
                 85.26127 
                 321.7737 
                 476.5288 
               
               
                 DU145_2 
                 10 
                 2 
                 A 
                 DU145 
                 5171.311 
                 40.9894 
                 187.2218 
                 62.03801 
                 1350.435 
                 73.11622 
                 820.8958 
                 1113.361 
               
               
                 LNCaP_23 
                 10 
                 2 
                 A 
                 LNCaP 
                 6032.05 
                 53.47256 
                 196.641 
                 134.5439 
                 705.4928 
                 60.37225 
                 464.0038 
                 679.619 
               
               
                 22RV1_5 
                 10 
                 2 
                 A 
                 22RV1 
                 5267.057 
                 18.41935 
                 207.8755 
                 102.6221 
                 545.5636 
                 175.4224 
                 621.8724 
                 892.8999 
               
               
                 PC3_5 
                 10 
                 2 
                 A 
                 PC3 
                 5504.31 
                 7.704801 
                 338.2408 
                 91.68713 
                 1168.048 
                 85.52329 
                 336.6998 
                 506.2054 
               
               
                 DU145_5 
                 10 
                 2 
                 A 
                 DU145 
                 6664.689 
                 49.88539 
                 160.631 
                 51.88081 
                 1336.929 
                 83.80746 
                 832.0884 
                 1152.353 
               
               
                 PANC10_6 
                 10 
                 2 
                 A 
                 PANC10 
                 7171.436 
                 6.719546 
                 202.8463 
                 88.19404 
                 308.6792 
                 103.733 
                 1004.992 
                 487.5871 
               
               
                 LNCaP_24 
                 10 
                 2 
                 A 
                 LNCaP 
                 6097.945 
                 69.42881 
                 182.0869 
                 142.7876 
                 865.8952 
                 94.31838 
                 427.0527 
                 685.1183 
               
               
                 22RV1_8 
                 10 
                 2 
                 A 
                 22RV1 
                 6350.482 
                 13.66185 
                 184.4349 
                 98.66889 
                 553.3048 
                 172.2911 
                 617.0601 
                 900.9229 
               
               
                 PC3_8 
                 10 
                 2 
                 A 
                 PC3 
                 4895.822 
                 13.63515 
                 320.025 
                 119.5081 
                 1160.592 
                 82.61298 
                 361.7326 
                 405.8463 
               
               
                 DU145_8 
                 10 
                 2 
                 A 
                 DU145 
                 6087.1 
                 52.70647 
                 264.5268 
                 81.54586 
                 1264.955 
                 48.72862 
                 782.6413 
                 959.6555 
               
               
                 PC3_PSI_4 
                 20 
                 0.5 
                 A 
                 PC3 
                 4525.786 
                 36.1925 
                 521.6316 
                 144.1955 
                 994.4321 
                 145.919 
                 503.8226 
                 238.9854 
               
               
                 PC3_PSI_7 
                 20 
                 2 
                 A 
                 PC3 
                 8340.647 
                 52.42027 
                 316.7618 
                 69.44565 
                 1302.89 
                 161.7412 
                 813.1862 
                 346.7802 
               
               
                 PC3_PSI_10 
                 20 
                 5 
                 A 
                 PC3 
                 7532.104 
                 33.96975 
                 339.2385 
                 64.72615 
                 1331.247 
                 147.3553 
                 691.3304 
                 316.2859 
               
               
                 PANC10_11 
                 10 
                 5 
                 B 
                 PANC10 
                 6243.027 
                 2.754277 
                 179.9461 
                 80.79211 
                 329.5951 
                 76.20165 
                 1043.871 
                 448.9471 
               
               
                 LNCaP_2 
                 10 
                 5 
                 B 
                 LNCaP 
                 5325.329 
                 26.73217 
                 175.8695 
                 112.5565 
                 699.2572 
                 68.94085 
                 448.819 
                 583.8868 
               
               
                 22RV1_11 
                 10 
                 5 
                 B 
                 22RV1 
                 3995.158 
                 9.30438 
                 193.2448 
                 106.6425 
                 491.7007 
                 130.977 
                 642.0022 
                 775.8422 
               
               
                 DU145_11 
                 10 
                 5 
                 B 
                 DU145 
                 9470.159 
                 52.89278 
                 228.0267 
                 106.961 
                 1691.394 
                 128.1181 
                 584.1714 
                 1290.584 
               
               
                 PANC10_14 
                 10 
                 5 
                 B 
                 PANC10 
                 6439.549 
                 6.723622 
                 181.5378 
                 82.36437 
                 305.0843 
                 90.76889 
                 1093.429 
                 451.3231 
               
               
                 LNCaP_5 
                 10 
                 5 
                 B 
                 LNCaP 
                 6890.206 
                 63.68512 
                 225.0789 
                 134.3494 
                 1019.834 
                 81.1331 
                 418.7515 
                 727.5807 
               
               
                 22RV1_14 
                 10 
                 5 
                 B 
                 22RV1 
                 4622.746 
                 11.10621 
                 210.1637 
                 109.3534 
                 473.2954 
                 134.9832 
                 633.054 
                 762.9112 
               
               
                 PC3_14 
                 10 
                 5 
                 B 
                 PC3 
                 4628.573 
                 14.94092 
                 275.162 
                 75.32715 
                 1221.42 
                 89.64553 
                 369.1653 
                 520.4421 
               
               
                 DU145_14 
                 10 
                 5 
                 B 
                 DU145 
                 12797.08 
                 55.05151 
                 192.6803 
                 71.80632 
                 1505.539 
                 106.5127 
                 578.0409 
                 1055.553 
               
               
                 PANC10_17 
                 10 
                 5 
                 B 
                 PANC10 
                 5897.427 
                 2.835574 
                 199.0573 
                 86.76856 
                 395.8461 
                 67.48666 
                 1159.183 
                 498.4939 
               
               
                 LNCaP_8 
                 10 
                 5 
                 B 
                 LNCaP 
                 5078.682 
                 37.93094 
                 188.3 
                 125.9849 
                 800.6138 
                 75.86188 
                 466.0087 
                 606.8951 
               
               
                 22RV1_17 
                 10 
                 5 
                 B 
                 22RV1 
                 3329.879 
                 9.25701 
                 259.1963 
                 100.5047 
                 403.3411 
                 148.1122 
                 536.9066 
                 720.7243 
               
               
                 PC3_17 
                 10 
                 5 
                 B 
                 PC3 
                 5029.755 
                 11.64969 
                 297.7953 
                 112.8564 
                 1199.19 
                 74.9949 
                 363.3248 
                 450.6975 
               
               
                 DU145_17 
                 10 
                 5 
                 B 
                 DU145 
                 6037.653 
                 24.16819 
                 253.766 
                 110.954 
                 1286.407 
                 48.33638 
                 781.0719 
                 853.5765 
               
               
                 PC3_PSI_5 
                 20 
                 0.5 
                 B 
                 PC3 
                 4611.407 
                 27.1003 
                 517.8093 
                 139.373 
                 1029.328 
                 146.1481 
                 630.082 
                 248.2581 
               
               
                 PC3_PSI_8 
                 20 
                 2 
                 B 
                 PC3 
                 8268.147 
                 33.7258 
                 360.3336 
                 72.77673 
                 1231.879 
                 170.4041 
                 657.6531 
                 305.3073 
               
               
                 PC3_PSI_11 
                 20 
                 5 
                 B 
                 PC3 
                 7663.484 
                 32.0363 
                 309.5508 
                 67.67669 
                 1285.457 
                 151.3715 
                 676.3664 
                 354.0011 
               
               
                 PANC10_19 
                 20 
                 2 
                 C 
                 PANC10 
                 6286.898 
                 8.007088 
                 149.4656 
                 72.06379 
                 344.3048 
                 109.4302 
                 1024.907 
                 464.4111 
               
               
                 PANC10_20 
                 20 
                 5 
                 C 
                 PANC10 
                 5962.61 
                 7.378707 
                 135.8912 
                 77.47642 
                 336.9609 
                 91.61894 
                 988.7467 
                 509.7457 
               
               
                 LNCaP_11 
                 20 
                 2 
                 C 
                 LNCaP 
                 5936.025 
                 74.10768 
                 123.0188 
                 105.2329 
                 812.2202 
                 111.1615 
                 545.4325 
                 690.6836 
               
               
                 LNCaP_12 
                 20 
                 5 
                 C 
                 LNCaP 
                 4783.436 
                 38.32323 
                 184.6483 
                 146.3251 
                 769.9486 
                 73.16253 
                 426.7815 
                 661.9467 
               
               
                 22RV1_19 
                 20 
                 2 
                 C 
                 22RV1 
                 4621.767 
                 16.70363 
                 156.7572 
                 104.0765 
                 535.8011 
                 179.8853 
                 719.5411 
                 846.7457 
               
               
                 PC3_19 
                 20 
                 5 
                 C 
                 PC3 
                 4385.726 
                 15.90183 
                 302.1348 
                 111.3128 
                 1166.488 
                 111.3128 
                 318.39 
                 461.5065 
               
               
                 PC3_22 
                 20 
                 2 
                 C 
                 PC3 
                 5214.032 
                 23.44097 
                 259.1178 
                 104.5341 
                 1242.371 
                 128.6086 
                 333.8754 
                 611.9994 
               
               
                 DU145_19 
                 20 
                 2 
                 C 
                 DU145 
                 5413.928 
                 16.60396 
                 189.9078 
                 64.34034 
                 1230.768 
                 47.73638 
                 777.2728 
                 801.141 
               
               
                 DU145_20 
                 20 
                 5 
                 C 
                 DU145 
                 6244.554 
                 21.75458 
                 173.0478 
                 78.11873 
                 1342.851 
                 51.41992 
                 997.7443 
                 972.0343 
               
               
                 PANC10_22 
                 20 
                 2 
                 C 
                 PANC10 
                 7881.479 
                 8.709063 
                 133.204 
                 64.31308 
                 340.3234 
                 104.0621 
                 1066.749 
                 517.1844 
               
               
                 PANC10_23 
                 20 
                 5 
                 C 
                 PANC10 
                 10103.54 
                 11.41336 
                 109.7856 
                 52.71885 
                 382.0758 
                 135.8733 
                 1322.863 
                 652.192 
               
               
                 LNCaP_14 
                 20 
                 2 
                 C 
                 LNCaP 
                 4922.166 
                 37.16044 
                 170.938 
                 128.469 
                 864.2458 
                 57.33326 
                 455.4809 
                 587.135 
               
               
                 LNCaP_15 
                 20 
                 5 
                 C 
                 LNCaP 
                 5734.62 
                 37.47328 
                 198.2457 
                 161.9813 
                 934.4143 
                 59.23196 
                 442.4264 
                 685.3983 
               
               
                 22RV1_22 
                 20 
                 2 
                 C 
                 22RV1 
                 4016.466 
                 11.28573 
                 208.159 
                 116.6192 
                 448.9212 
                 152.9843 
                 560.5245 
                 753.6359 
               
               
                 22RV1_23 
                 20 
                 5 
                 C 
                 22RV1 
                 4193.949 
                 7.02112 
                 208.2932 
                 106.487 
                 463.3939 
                 161.4858 
                 599.1356 
                 753.6002 
               
               
                 PC3_20 
                 20 
                 5 
                 C 
                 PC3 
                 4357.359 
                 9.31913 
                 295.5495 
                 114.4922 
                 1065.043 
                 102.5104 
                 340.8139 
                 451.3121 
               
               
                 PC3_23 
                 20 
                 2 
                 C 
                 PC3 
                 4707.749 
                 18.05044 
                 255.6332 
                 97.56992 
                 1064.976 
                 103.912 
                 384.4255 
                 515.657 
               
               
                 DU145_22 
                 20 
                 2 
                 C 
                 DU145 
                 4967.309 
                 18.83786 
                 289.6321 
                 83.59301 
                 1186.785 
                 28.25679 
                 676.9857 
                 766.4655 
               
               
                 DU145_23 
                 20 
                 5 
                 C 
                 DU145 
                 5566.042 
                 17.69525 
                 282.2393 
                 107.0563 
                 1304.14 
                 45.1229 
                 820.175 
                 728.1597 
               
               
                 PANC10_26 
                 20 
                 5 
                 C 
                 PANC10 
                 8129.403 
                 9.119588 
                 163.7473 
                 72.34873 
                 346.5443 
                 113.4882 
                 1121.507 
                 516.1687 
               
               
                 LNCaP_17 
                 20 
                 2 
                 C 
                 LNCaP 
                 6488.608 
                 32.99507 
                 156.0921 
                 110.4066 
                 736.0439 
                 72.33535 
                 445.4335 
                 588.8351 
               
               
                 LNCaP_18 
                 20 
                 5 
                 C 
                 LNCaP 
                 7181.955 
                 52.25351 
                 197.4021 
                 138.375 
                 1079.906 
                 64.83306 
                 566.0797 
                 755.7406 
               
               
                 22RV1_25 
                 20 
                 2 
                 C 
                 22RV1 
                 5585.949 
                 20.47385 
                 206.4447 
                 98.95695 
                 440.1878 
                 170.6154 
                 590.3294 
                 766.0633 
               
               
                 22RV1_26 
                 20 
                 5 
                 C 
                 22RV1 
                 5535.944 
                 9.630521 
                 195.8206 
                 104.3306 
                 455.8446 
                 216.6867 
                 563.3855 
                 781.6773 
               
               
                 PC3_21 
                 20 
                 5 
                 C 
                 PC3 
                 4587.455 
                 12.34116 
                 311.4908 
                 131.3099 
                 1080.592 
                 81.94528 
                 348.5143 
                 459.091 
               
               
                 PC3_24 
                 20 
                 2 
                 C 
                 PC3 
                 4373.948 
                 14.64492 
                 308.0314 
                 144.4965 
                 1114.478 
                 87.86949 
                 330.9751 
                 424.2144 
               
               
                 DU145_25 
                 20 
                 2 
                 C 
                 DU145 
                 6117.638 
                 22.03772 
                 206.3632 
                 86.722 
                 1293.521 
                 36.49138 
                 824.7932 
                 820.0669 
               
               
                 DU145_26 
                 20 
                 5 
                 C 
                 DU145 
                 2894.176 
                 0 
                 241.1813 
                 0 
                 241.1813 
                 0 
                 0 
                 2652.994 
               
               
                 PC3_PSI_6 
                 20 
                 0.5 
                 C 
                 PC3 
                 4629.7 
                 26.60747 
                 476.1337 
                 166.6468 
                 981.6756 
                 166.6468 
                 674.9895 
                 253.4712 
               
               
                 PC3_PSI_9 
                 20 
                 2 
                 C 
                 PC3 
                 7738.04 
                 31.81803 
                 320.7883 
                 68.85212 
                 1257.594 
                 162.7414 
                 730.2498 
                 336.4365 
               
               
                 PC3_PSI_12 
                 20 
                 5 
                 C 
                 PC3 
                 6790.805 
                 28.69824 
                 326.3591 
                 79.42071 
                 1058.832 
                 142.8238 
                 721.7941 
                 265.9593 
               
               
                 PANC10_1 
                 1 
                 2 
                 A 
                 PANC10 
                 6542.985 
                 15.66557 
                 177.5431 
                 31.33113 
                 344.6425 
                 20.88742 
                 1206.249 
                 386.4173 
               
               
                 LNCaP_19 
                 1 
                 2 
                 A 
                 LNCaP 
                 6474.671 
                 48.07924 
                 179.2044 
                 88.87375 
                 687.6788 
                 67.01955 
                 543.4411 
                 619.2023 
               
               
                 22RV1_1 
                 1 
                 2 
                 A 
                 22RV1 
                 4530.578 
                 25.75005 
                 167.3753 
                 90.12517 
                 497.8343 
                 175.9587 
                 716.7097 
                 1032.863 
               
               
                 PC3_1 
                 1 
                 2 
                 A 
                 PC3 
                 5083.18 
                 13.56792 
                 287.3206 
                 63.84902 
                 1140.503 
                 91.78297 
                 339.996 
                 620.1336 
               
               
                 DU145_1 
                 1 
                 2 
                 A 
                 DU145 
                 4611.349 
                 23.01838 
                 203.8771 
                 76.72793 
                 1121.324 
                 52.61344 
                 643.4185 
                 842.9111 
               
               
                 PANC10_2 
                 1 
                 2 
                 A 
                 PANC10 
                 9006.806 
                 11.57364 
                 198.2614 
                 81.51866 
                 371.3628 
                 150.9605 
                 1150.822 
                 517.7945 
               
               
                 22RV1_4 
                 1 
                 2 
                 A 
                 22RV1 
                 6490.111 
                 11.6954 
                 183.7848 
                 99.41089 
                 521.2806 
                 182.9495 
                 675.827 
                 860.4472 
               
               
                 PC3_4 
                 1 
                 2 
                 A 
                 PC3 
                 6749.623 
                 17.98637 
                 247.076 
                 81.412 
                 1196.567 
                 88.03856 
                 360.6741 
                 476.1655 
               
               
                 DU145_4 
                 1 
                 2 
                 A 
                 DU145 
                 5878.828 
                 43.86276 
                 194.9456 
                 86.50711 
                 1046.614 
                 71.88619 
                 661.5966 
                 952.7966 
               
               
                 PANC10_3 
                 1 
                 2 
                 A 
                 PANC10 
                 7205.48 
                 7.480773 
                 187.0193 
                 71.81542 
                 275.2924 
                 112.2116 
                 975.4928 
                 409.9463 
               
               
                 LNCaP_21 
                 1 
                 2 
                 A 
                 LNCaP 
                 6624.831 
                 31.06272 
                 189.2002 
                 121.427 
                 796.3352 
                 50.82991 
                 454.6453 
                 810.4547 
               
               
                 22RV1_7 
                 1 
                 2 
                 A 
                 22RV1 
                 6886.516 
                 12.73092 
                 185.5076 
                 92.75382 
                 509.2367 
                 176.4141 
                 649.2767 
                 845.6966 
               
               
                 PC3_7 
                 1 
                 2 
                 A 
                 PC3 
                 6622.96 
                 18.76547 
                 282.7331 
                 78.18946 
                 1148.447 
                 76.31292 
                 363.4246 
                 402.8321 
               
               
                 DU145_7 
                 1 
                 2 
                 A 
                 DU145 
                 6479.28 
                 55.70184 
                 243.8269 
                 102.9958 
                 1239.103 
                 58.85477 
                 715.716 
                 903.8411 
               
               
                 PANC10_10 
                 1 
                 5 
                 B 
                 PANC10 
                 6992.427 
                 6.695589 
                 189.7084 
                 70.30368 
                 302.4174 
                 104.8976 
                 1035.584 
                 464.2275 
               
               
                 LNCaP_3 
                 1 
                 5 
                 B 
                 LNCaP 
                 4050.935 
                 20.71155 
                 221.7354 
                 194.9322 
                 670.0794 
                 70.66292 
                 397.1744 
                 556.7751 
               
               
                 22RV1_10 
                 1 
                 5 
                 B 
                 22RV1 
                 3566.743 
                 13.4891 
                 256.2929 
                 130.3947 
                 465.374 
                 145.0078 
                 537.3159 
                 657.5937 
               
               
                 DU145_10 
                 1 
                 5 
                 B 
                 DU145 
                 7010.738 
                 26.52666 
                 258.421 
                 85.56986 
                 1233.917 
                 72.73438 
                 604.9789 
                 1029.405 
               
               
                 PANC10_13 
                 1 
                 5 
                 B 
                 PANC10 
                 7888.156 
                 3.634258 
                 158.9988 
                 53.6053 
                 268.9351 
                 99.94208 
                 1051.209 
                 480.6306 
               
               
                 LNCaP_6 
                 1 
                 5 
                 B 
                 LNCaP 
                 4510.219 
                 31.42167 
                 209.075 
                 196.9897 
                 685.2342 
                 51.96662 
                 404.8562 
                 663.4808 
               
               
                 22RV1_13 
                 1 
                 5 
                 B 
                 22RV1 
                 4500.345 
                 13.61203 
                 276.4288 
                 110.9904 
                 468.0443 
                 127.7436 
                 639.7652 
                 774.8384 
               
               
                 PC3_13 
                 1 
                 5 
                 B 
                 PC3 
                 5296.603 
                 20.02733 
                 262.2329 
                 83.23859 
                 1182.238 
                 103.2659 
                 369.2539 
                 473.7715 
               
               
                 DU145_13 
                 1 
                 5 
                 B 
                 DU145 
                 10515.06 
                 27.92189 
                 192.1683 
                 54.20132 
                 1005.188 
                 93.62045 
                 796.5951 
                 850.7964 
               
               
                 PANC10_16 
                 1 
                 5 
                 B 
                 PANC10 
                 5799.396 
                 17.01638 
                 226.5306 
                 57.43029 
                 307.3584 
                 82.95487 
                 916.7577 
                 398.8215 
               
               
                 LNCaP_9 
                 1 
                 5 
                 B 
                 LNCaP 
                 4866.622 
                 33.71794 
                 204.5555 
                 176.4572 
                 904.7646 
                 60.69229 
                 400.1195 
                 714.8203 
               
               
                 22RV1_16 
                 1 
                 5 
                 B 
                 22RV1 
                 3998.346 
                 9.453951 
                 249.8544 
                 94.53951 
                 292.3972 
                 145.1857 
                 644.8945 
                 759.0172 
               
               
                 PC3_16 
                 1 
                 5 
                 B 
                 PC3 
                 5563.723 
                 20.61274 
                 300.0299 
                 92.18477 
                 1156.031 
                 75.58006 
                 368.7391 
                 382.4809 
               
               
                 DU145_16 
                 1 
                 5 
                 B 
                 DU145 
                 8976.212 
                 7.204023 
                 198.1106 
                 59.43319 
                 1197.669 
                 5.403017 
                 1298.525 
                 617.7449 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 15 
               
             
            
               
                   
               
               
                 Pressure-Sensitive Candidates 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 O2 
                 PSI 
                 Expt 
                 CellLine 
                 SMYD3 
                 EIF4A2 
                 DDHD2 
                 PDIA5 
                 COPG2 
                 TNFRSF13C 
                 SLC6A6 
                 NRAV 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 LNCaP_25 
                 20 
                 0 
                 A 
                 LNCaP 
                 216.5306 
                 4092.09 
                 707.1077 
                 466.8941 
                 776.4651 
                 16.91645 
                 159.0146 
                 209.764 
               
               
                 22RV1_3 
                 20 
                 0 
                 A 
                 22RV1 
                 226.3622 
                 2118.965 
                 530.4109 
                 324.14 
                 358.9649 
                 57.59512 
                 349.589 
                 322.8006 
               
               
                 PC3_3 
                 20 
                 0 
                 A 
                 PC3 
                 301.5071 
                 2661.077 
                 534.7814 
                 355.1602 
                 315.5035 
                 2.915929 
                 1007.162 
                 145.2133 
               
               
                 DU145_3 
                 20 
                 0 
                 A 
                 DU145 
                 398.101 
                 4582.536 
                 999.6273 
                 230.7674 
                 550.1231 
                 4.374737 
                 1817.703 
                 283.2642 
               
               
                 PANC10_8 
                 20 
                 0 
                 A 
                 PANC10 
                 247.8738 
                 3734.471 
                 274.54 
                 177.5722 
                 453.3242 
                 0.606048 
                 451.5061 
                 223.0258 
               
               
                 LNCaP_26 
                 20 
                 0 
                 A 
                 LNCaP 
                 191.5879 
                 4698.465 
                 738.9819 
                 479.8821 
                 773.6502 
                 7.298587 
                 120.4267 
                 193.4126 
               
               
                 22RV1_6 
                 20 
                 0 
                 A 
                 22RV1 
                 234.1377 
                 1957.309 
                 602.8019 
                 281.376 
                 379.9603 
                 83.1805 
                 337.8566 
                 255.703 
               
               
                 PC3_6 
                 20 
                 0 
                 A 
                 PC3 
                 310.1101 
                 3292.726 
                 553.6636 
                 399.3399 
                 372.2784 
                 3.656959 
                 895.955 
                 130.9191 
               
               
                 DU145_6 
                 20 
                 0 
                 A 
                 DU145 
                 377.5716 
                 4293.725 
                 1016.45 
                 212.9596 
                 572.113 
                 6.906797 
                 1722.095 
                 310.8059 
               
               
                 PANC10_9 
                 20 
                 0 
                 A 
                 PANC10 
                 149.3481 
                 3389.552 
                 334.4098 
                 185.0618 
                 444.7976 
                 0 
                 389.6037 
                 204.5419 
               
               
                 LNCaP_27 
                 20 
                 0 
                 A 
                 LNCaP 
                 268.0917 
                 3942.762 
                 608.7496 
                 544.2464 
                 741.7876 
                 8.062909 
                 133.038 
                 237.8558 
               
               
                 22RV1_9 
                 20 
                 0 
                 A 
                 22RV1 
                 254.5334 
                 2052.688 
                 578.8581 
                 328.4301 
                 387.9581 
                 59.52796 
                 398.2215 
                 240.1645 
               
               
                 PC3_9 
                 20 
                 0 
                 A 
                 PC3 
                 346.5156 
                 3502.103 
                 538.5267 
                 361.6302 
                 401.376 
                 3.918594 
                 870.4876 
                 130.4332 
               
               
                 DU145_9 
                 20 
                 0 
                 A 
                 DU145 
                 375.1292 
                 3681.986 
                 1170.491 
                 178.2139 
                 694.154 
                 7.700599 
                 2293.678 
                 276.1215 
               
               
                 PC3_PSI_1 
                 20 
                 0 
                 A 
                 PC3 
                 557.0377 
                 5043.206 
                 582.7365 
                 510.5022 
                 357.0042 
                 4.861925 
                 977.247 
                 120.159 
               
               
                 PANC10_12 
                 20 
                 0 
                 B 
                 PANC10 
                 198.7211 
                 2358.25 
                 244.5798 
                 177.8762 
                 484.9907 
                 2.779316 
                 518.3425 
                 201.5004 
               
               
                 LNCaP_1 
                 20 
                 0 
                 B 
                 LNCaP 
                 211.1472 
                 4754.218 
                 711.7705 
                 463.1617 
                 779.8825 
                 14.7576 
                 148.7112 
                 174.8208 
               
               
                 22RV1_12 
                 20 
                 0 
                 B 
                 22RV1 
                 210.292 
                 2571.622 
                 549.7889 
                 320.7844 
                 372.4664 
                 65.93902 
                 400.0895 
                 326.1308 
               
               
                 DU145_12 
                 20 
                 0 
                 B 
                 DU145 
                 449.716 
                 6546.37 
                 835.996 
                 164.254 
                 408.9357 
                 5.663929 
                 1144.114 
                 306.985 
               
               
                 PANC10_15 
                 20 
                 0 
                 B 
                 PANC10 
                 201.4568 
                 3136.808 
                 349.4194 
                 188.9369 
                 503.0729 
                 2.276348 
                 413.1572 
                 180.9697 
               
               
                 LNCaP_4 
                 20 
                 0 
                 B 
                 LNCaP 
                 243.9977 
                 3823.079 
                 562.1383 
                 571.5746 
                 757.606 
                 13.48053 
                 136.1534 
                 186.0314 
               
               
                 22RV1_15 
                 20 
                 0 
                 B 
                 22RV1 
                 211.526 
                 2394.045 
                 582.9148 
                 311.9277 
                 357.742 
                 57.51166 
                 386.9853 
                 297.3061 
               
               
                 PC3_15 
                 20 
                 0 
                 B 
                 PC3 
                 319.4519 
                 2975.404 
                 522.8381 
                 355.7902 
                 319.4519 
                 3.796542 
                 984.9314 
                 167.5902 
               
               
                 DU145_15 
                 20 
                 0 
                 B 
                 DU145 
                 377.0446 
                 4683.821 
                 805.5043 
                 188.5223 
                 523.225 
                 3.024422 
                 1185.573 
                 243.97 
               
               
                 PANC10_18 
                 20 
                 0 
                 B 
                 PANC10 
                 190.6705 
                 3178.938 
                 317.2363 
                 184.0957 
                 567.0805 
                 1.643711 
                 427.365 
                 198.8891 
               
               
                 LNCaP_7 
                 20 
                 0 
                 B 
                 LNCaP 
                 220.6287 
                 4329.968 
                 680.7074 
                 508.1779 
                 775.8601 
                 3.1369 
                 93.06138 
                 181.9402 
               
               
                 22RV1_18 
                 20 
                 0 
                 B 
                 22RV1 
                 225.9862 
                 1863.102 
                 626.5982 
                 306.879 
                 340.2634 
                 48.79248 
                 430.1442 
                 273.4947 
               
               
                 PC3_18 
                 20 
                 0 
                 B 
                 PC3 
                 349.1251 
                 3015.602 
                 569.6964 
                 374.1593 
                 349.1251 
                 4.736194 
                 847.1021 
                 135.9964 
               
               
                 DU145_18 
                 20 
                 0 
                 B 
                 DU145 
                 406.9059 
                 3251.63 
                 768.6 
                 226.0588 
                 503.6591 
                 15.372 
                 1249.653 
                 245.952 
               
               
                 PC3_PSI_2 
                 20 
                 0 
                 B 
                 PC3 
                 629.4451 
                 5833.865 
                 511.8348 
                 660.983 
                 308.1521 
                 2.628163 
                 599.2212 
                 164.2602 
               
               
                 PANC10_21 
                 20 
                 0 
                 C 
                 PANC10 
                 219.9357 
                 3093.674 
                 298.9889 
                 173.1221 
                 557.7888 
                 2.649828 
                 340.9445 
                 158.1064 
               
               
                 LNCaP_10 
                 20 
                 0 
                 C 
                 LNCaP 
                 286.8137 
                 3509.721 
                 533.6534 
                 576.6255 
                 826.4632 
                 9.993509 
                 147.9039 
                 196.8721 
               
               
                 22RV1_21 
                 20 
                 0 
                 C 
                 22RV1 
                 186.9129 
                 1934.96 
                 625.3941 
                 345.6125 
                 403.2146 
                 70.53317 
                 237.4617 
                 303.2926 
               
               
                 PC3_25 
                 20 
                 0 
                 C 
                 PC3 
                 334.6433 
                 2751.511 
                 627.9726 
                 356.9528 
                 349.5163 
                 0.82628 
                 581.7009 
                 140.4676 
               
               
                 DU145_21 
                 20 
                 0 
                 C 
                 DU145 
                 350.3576 
                 4212.368 
                 971.3083 
                 209.0029 
                 442.2381 
                 5.04838 
                 1489.272 
                 284.7286 
               
               
                 PANC10_24 
                 20 
                 0 
                 C 
                 PANC10 
                 200.9849 
                 2964.754 
                 299.2037 
                 185.5245 
                 438.8018 
                 3.637735 
                 426.0697 
                 188.7075 
               
               
                 LNCaP_13 
                 20 
                 0 
                 C 
                 LNCaP 
                 218.8769 
                 3543.216 
                 601.323 
                 557.783 
                 776.6598 
                 11.76757 
                 85.90328 
                 245.9423 
               
               
                 22RV1_24 
                 20 
                 0 
                 C 
                 22RV1 
                 178.955 
                 2172.783 
                 612.8364 
                 324.989 
                 344.4039 
                 54.02414 
                 277.7179 
                 266.7442 
               
               
                 PC3_26 
                 20 
                 0 
                 C 
                 PC3 
                 307.9801 
                 2208.218 
                 486.6086 
                 331.0787 
                 298.7407 
                 2.309851 
                 722.2134 
                 145.5206 
               
               
                 DU145_24 
                 20 
                 0 
                 C 
                 DU145 
                 325.6477 
                 3784.642 
                 821.4098 
                 223.579 
                 429.3365 
                 12.9611 
                 1370.636 
                 262.4623 
               
               
                 PANC10_27 
                 20 
                 0 
                 C 
                 PANC10 
                 197.1584 
                 2083.021 
                 291.4515 
                 269.164 
                 444.0349 
                 3.428841 
                 402.8888 
                 207.4449 
               
               
                 LNCaP_16 
                 20 
                 0 
                 C 
                 LNCaP 
                 264.5144 
                 3108.354 
                 661.9068 
                 571.2517 
                 746.3527 
                 8.69296 
                 136.6037 
                 212.3566 
               
               
                 22RV1_27 
                 20 
                 0 
                 C 
                 22RV1 
                 222.8237 
                 2059.094 
                 600.6113 
                 346.3896 
                 342.3383 
                 53.68026 
                 260.2986 
                 325.1201 
               
               
                 PC3_27 
                 20 
                 0 
                 C 
                 PC3 
                 347.1039 
                 2788.642 
                 591.8483 
                 362.8516 
                 372.0377 
                 3.936907 
                 576.1007 
                 133.8548 
               
               
                 DU145_27 
                 20 
                 0 
                 C 
                 DU145 
                 0 
                 3984.754 
                 664.1257 
                 110.6876 
                 0 
                 0 
                 1549.627 
                 110.6876 
               
               
                 PC3_PSI_3 
                 20 
                 0 
                 C 
                 PC3 
                 514.207 
                 5309.227 
                 709.2236 
                 547.6384 
                 350.2339 
                 0 
                 949.6114 
                 183.8728 
               
               
                 PANC10_4 
                 10 
                 2 
                 A 
                 PANC10 
                 172.5885 
                 2530.92 
                 493.9211 
                 229.3611 
                 468.9412 
                 6.812705 
                 420.1168 
                 158.9631 
               
               
                 PC3_2 
                 10 
                 2 
                 A 
                 PC3 
                 331.7014 
                 2527.471 
                 597.4129 
                 463.6812 
                 364.4044 
                 5.255832 
                 1190.738 
                 99.86081 
               
               
                 DU145_2 
                 10 
                 2 
                 A 
                 DU145 
                 488.5493 
                 2515.863 
                 1301.69 
                 334.5621 
                 672.4477 
                 27.69554 
                 2384.032 
                 151.7716 
               
               
                 LNCaP_23 
                 10 
                 2 
                 A 
                 LNCaP 
                 303.5862 
                 2847.845 
                 907.3086 
                 488.1527 
                 1010.804 
                 20.69906 
                 222.5149 
                 196.641 
               
               
                 22RV1_5 
                 10 
                 2 
                 A 
                 22RV1 
                 235.9431 
                 1506.878 
                 785.8923 
                 355.2303 
                 416.6282 
                 275.4131 
                 532.4069 
                 209.6298 
               
               
                 PC3_5 
                 10 
                 2 
                 A 
                 PC3 
                 339.7817 
                 2495.585 
                 703.4483 
                 463.0585 
                 459.2061 
                 3.08192 
                 1162.654 
                 124.8178 
               
               
                 DU145_5 
                 10 
                 2 
                 A 
                 DU145 
                 426.0213 
                 2630.956 
                 1326.951 
                 343.2115 
                 786.1938 
                 26.93811 
                 2799.568 
                 157.6378 
               
               
                 PANC10_6 
                 10 
                 2 
                 A 
                 PANC10 
                 254.5028 
                 2378.719 
                 462.3888 
                 212.5056 
                 626.5977 
                 6.719546 
                 646.3363 
                 150.3498 
               
               
                 LNCaP_24 
                 10 
                 2 
                 A 
                 LNCaP 
                 313.0846 
                 2703.794 
                 1015.233 
                 466.352 
                 994.273 
                 26.19955 
                 256.7556 
                 192.5667 
               
               
                 22RV1_8 
                 10 
                 2 
                 A 
                 22RV1 
                 283.8628 
                 1546.825 
                 713.452 
                 379.4957 
                 428.8302 
                 207.2047 
                 531.294 
                 222.3845 
               
               
                 PC3_8 
                 10 
                 2 
                 A 
                 PC3 
                 404.2422 
                 2866.59 
                 692.9866 
                 372.1595 
                 535.7813 
                 1.604136 
                 1111.666 
                 117.904 
               
               
                 DU145_8 
                 10 
                 2 
                 A 
                 DU145 
                 435.5742 
                 3022.169 
                 1509.593 
                 266.5157 
                 802.5306 
                 21.87816 
                 2961.507 
                 160.1083 
               
               
                 PC3_PSI_4 
                 20 
                 0.5 
                 A 
                 PC3 
                 595.1656 
                 5259.403 
                 772.1067 
                 695.1259 
                 476.8219 
                 4.021389 
                 2031.376 
                 129.8334 
               
               
                 PC3_PSI_7 
                 20 
                 2 
                 A 
                 PC3 
                 921.611 
                 2887.595 
                 943.1167 
                 745.0846 
                 798.849 
                 4.032328 
                 1392.945 
                 105.7366 
               
               
                 PC3_PSI_10 
                 20 
                 5 
                 A 
                 PC3 
                 863.9334 
                 3103.642 
                 885.5088 
                 649.5568 
                 779.9272 
                 2.754304 
                 1422.598 
                 105.5817 
               
               
                 PANC10_11 
                 10 
                 5 
                 B 
                 PANC10 
                 248.803 
                 2225.455 
                 291.9533 
                 206.5707 
                 500.3602 
                 1.836184 
                 610.5313 
                 154.2395 
               
               
                 LNCaP_2 
                 10 
                 5 
                 B 
                 LNCaP 
                 315.1582 
                 2425.592 
                 704.885 
                 606.3981 
                 945.4745 
                 15.47652 
                 219.4851 
                 215.2643 
               
               
                 22RV1_11 
                 10 
                 5 
                 B 
                 22RV1 
                 275.5528 
                 1928.87 
                 726.4574 
                 335.6734 
                 404.3827 
                 73.0036 
                 539.6541 
                 249.7868 
               
               
                 DU145_11 
                 10 
                 5 
                 B 
                 DU145 
                 550.085 
                 5159.985 
                 964.9995 
                 230.3775 
                 530.1032 
                 18.80632 
                 1224.762 
                 258.5869 
               
               
                 PANC10_14 
                 10 
                 5 
                 B 
                 PANC10 
                 227.7627 
                 2120.462 
                 394.1723 
                 242.8908 
                 531.1661 
                 10.08543 
                 562.2629 
                 193.3041 
               
               
                 LNCaP_5 
                 10 
                 5 
                 B 
                 LNCaP 
                 318.4256 
                 3359.608 
                 896.826 
                 488.5434 
                 977.0867 
                 8.723989 
                 189.3106 
                 153.5422 
               
               
                 22RV1_14 
                 10 
                 5 
                 B 
                 22RV1 
                 253.7342 
                 1765.887 
                 685.1677 
                 353.6901 
                 411.7841 
                 80.30644 
                 498.0708 
                 263.1317 
               
               
                 PC3_14 
                 10 
                 5 
                 B 
                 PC3 
                 479.9771 
                 2038.191 
                 673.5865 
                 437.6445 
                 359.2047 
                 6.847922 
                 1572.532 
                 115.1696 
               
               
                 DU145_14 
                 10 
                 5 
                 B 
                 DU145 
                 485.8895 
                 5380.687 
                 964.5983 
                 271.6673 
                 579.2377 
                 11.96772 
                 1443.307 
                 305.1769 
               
               
                 PANC10_17 
                 10 
                 5 
                 B 
                 PANC10 
                 218.3392 
                 2764.117 
                 390.7421 
                 242.7251 
                 570.5175 
                 4.536918 
                 538.1919 
                 156.5237 
               
               
                 LNCaP_8 
                 10 
                 5 
                 B 
                 LNCaP 
                 323.7677 
                 2504.797 
                 732.88 
                 551.3533 
                 842.6088 
                 13.54676 
                 199.1374 
                 224.8763 
               
               
                 22RV1_17 
                 10 
                 5 
                 B 
                 22RV1 
                 223.4907 
                 1539.308 
                 752.4626 
                 312.0935 
                 374.2477 
                 75.37851 
                 518.3925 
                 260.5187 
               
               
                 PC3_17 
                 10 
                 5 
                 B 
                 PC3 
                 427.3981 
                 2114.419 
                 707.7189 
                 485.6466 
                 407.0112 
                 4.368635 
                 1372.48 
                 105.5753 
               
               
                 DU145_17 
                 10 
                 5 
                 B 
                 DU145 
                 429.5346 
                 3315.436 
                 1151.285 
                 237.2877 
                 703.0746 
                 9.886987 
                 2092.746 
                 240.5833 
               
               
                 PC3_PSI_5 
                 20 
                 0.5 
                 B 
                 PC3 
                 617.4997 
                 4780.3 
                 776.2301 
                 741.3868 
                 489.2572 
                 0.967868 
                 1652.634 
                 138.4051 
               
               
                 PC3_PSI_8 
                 20 
                 2 
                 B 
                 PC3 
                 831.6073 
                 2816.104 
                 970.0606 
                 634.5776 
                 678.9537 
                 1.775042 
                 1510.561 
                 102.9524 
               
               
                 PC3_PSI_11 
                 20 
                 5 
                 B 
                 PC3 
                 789.2944 
                 2774.344 
                 937.4623 
                 469.7323 
                 840.9529 
                 4.004538 
                 1509.31 
                 109.3239 
               
               
                 PANC10_19 
                 20 
                 2 
                 C 
                 PANC10 
                 205.5153 
                 1706.844 
                 345.6393 
                 294.9277 
                 596.528 
                 2.669029 
                 591.19 
                 134.786 
               
               
                 PANC10_20 
                 20 
                 5 
                 C 
                 PANC10 
                 215.2123 
                 1885.26 
                 327.1227 
                 261.3292 
                 539.2605 
                 4.304246 
                 493.7585 
                 142.655 
               
               
                 LNCaP_11 
                 20 
                 2 
                 C 
                 LNCaP 
                 352.7526 
                 2647.126 
                 668.4513 
                 628.4331 
                 967.8463 
                 40.01815 
                 198.6086 
                 154.144 
               
               
                 LNCaP_12 
                 20 
                 5 
                 C 
                 LNCaP 
                 350.135 
                 3013.6 
                 632.3333 
                 578.3324 
                 989.4362 
                 17.41965 
                 162.0028 
                 158.5188 
               
               
                 22RV1_19 
                 20 
                 2 
                 C 
                 22RV1 
                 227.4264 
                 1484.054 
                 728.5354 
                 409.8815 
                 459.9924 
                 138.7686 
                 398.3174 
                 187.5946 
               
               
                 PC3_19 
                 20 
                 5 
                 C 
                 PC3 
                 372.8096 
                 2153.108 
                 664.3432 
                 437.1237 
                 400.3728 
                 5.300611 
                 855.872 
                 114.1398 
               
               
                 PC3_22 
                 20 
                 2 
                 C 
                 PC3 
                 391.5276 
                 1848.669 
                 741.2415 
                 496.0616 
                 390.894 
                 7.602477 
                 947.1419 
                 77.92539 
               
               
                 DU145_19 
                 20 
                 2 
                 C 
                 DU145 
                 479.4393 
                 3235.696 
                 935.0104 
                 265.6633 
                 516.7982 
                 8.301979 
                 1805.68 
                 216.8892 
               
               
                 DU145_20 
                 20 
                 5 
                 C 
                 DU145 
                 537.9315 
                 2679.769 
                 654.6152 
                 354.9952 
                 415.3148 
                 17.7992 
                 962.1459 
                 261.055 
               
               
                 PANC10_22 
                 20 
                 2 
                 C 
                 PANC10 
                 257.9223 
                 1890.76 
                 367.6788 
                 237.1545 
                 542.3067 
                 9.490646 
                 633.8635 
                 165.8072 
               
               
                 PANC10_23 
                 20 
                 5 
                 C 
                 PANC10 
                 285.8775 
                 2162.016 
                 347.8357 
                 238.0501 
                 548.9282 
                 7.608906 
                 436.4251 
                 174.4613 
               
               
                 LNCaP_14 
                 20 
                 2 
                 C 
                 LNCaP 
                 278.1725 
                 2702.095 
                 706.0484 
                 679.5053 
                 829.2088 
                 16.98763 
                 149.7035 
                 198.5429 
               
               
                 LNCaP_15 
                 20 
                 5 
                 C 
                 LNCaP 
                 268.357 
                 3457.212 
                 646.7162 
                 633.4193 
                 796.6094 
                 9.670523 
                 151.1019 
                 217.5868 
               
               
                 22RV1_22 
                 20 
                 2 
                 C 
                 22RV1 
                 209.413 
                 1636.431 
                 756.1439 
                 403.7783 
                 453.9371 
                 125.397 
                 412.5561 
                 263.3337 
               
               
                 22RV1_23 
                 20 
                 5 
                 C 
                 22RV1 
                 212.974 
                 1630.07 
                 704.4524 
                 362.7579 
                 394.3529 
                 97.1255 
                 370.9492 
                 270.3131 
               
               
                 PC3_20 
                 20 
                 5 
                 C 
                 PC3 
                 362.7804 
                 2094.142 
                 571.1295 
                 421.3578 
                 350.133 
                 7.987825 
                 983.1682 
                 110.4983 
               
               
                 PC3_23 
                 20 
                 2 
                 C 
                 PC3 
                 420.0385 
                 1821.143 
                 588.8345 
                 466.3842 
                 356.1302 
                 7.317744 
                 1072.293 
                 112.2054 
               
               
                 DU145_22 
                 20 
                 2 
                 C 
                 DU145 
                 402.6593 
                 4565.827 
                 1045.501 
                 275.5037 
                 487.4297 
                 25.90206 
                 2083.938 
                 209.5712 
               
               
                 DU145_23 
                 20 
                 5 
                 C 
                 DU145 
                 444.1509 
                 3486.85 
                 1136.92 
                 291.9717 
                 518.4709 
                 10.61715 
                 1947.363 
                 224.7297 
               
               
                 PANC10_26 
                 20 
                 5 
                 C 
                 PANC10 
                 220.8967 
                 2337.046 
                 395.3848 
                 232.0429 
                 567.441 
                 10.13288 
                 452.7369 
                 172.6642 
               
               
                 LNCaP_17 
                 20 
                 2 
                 C 
                 LNCaP 
                 300.7628 
                 2307.117 
                 713.2012 
                 626.9064 
                 930.2072 
                 24.11178 
                 214.468 
                 189.0871 
               
               
                 LNCaP_18 
                 20 
                 5 
                 C 
                 LNCaP 
                 315.4564 
                 3161.337 
                 919.2747 
                 575.7563 
                 941.5308 
                 15.48252 
                 158.6958 
                 165.4694 
               
               
                 22RV1_25 
                 20 
                 2 
                 C 
                 22RV1 
                 255.9232 
                 1339.331 
                 759.2387 
                 397.534 
                 414.5955 
                 112.6062 
                 433.3632 
                 288.3401 
               
               
                 22RV1_26 
                 20 
                 5 
                 C 
                 22RV1 
                 235.9478 
                 1407.661 
                 669.3212 
                 390.0361 
                 513.6278 
                 99.51538 
                 431.7683 
                 258.419 
               
               
                 PC3_21 
                 20 
                 5 
                 C 
                 PC3 
                 352.4634 
                 2255.47 
                 633.3481 
                 413.6756 
                 390.9678 
                 8.391986 
                 894.9806 
                 108.1085 
               
               
                 PC3_24 
                 20 
                 2 
                 C 
                 PC3 
                 386.1376 
                 2445.213 
                 680.5004 
                 411.5221 
                 436.9066 
                 3.905311 
                 845.4998 
                 122.5291 
               
               
                 DU145_25 
                 20 
                 2 
                 C 
                 DU145 
                 436.3578 
                 3645.017 
                 950.5346 
                 314.0787 
                 550.5033 
                 11.26617 
                 1721.8 
                 234.3912 
               
               
                 DU145_26 
                 20 
                 5 
                 C 
                 DU145 
                 0 
                 964.7252 
                 964.7252 
                 241.1813 
                 723.5439 
                 0 
                 1688.269 
                 241.1813 
               
               
                 PC3_PSI_6 
                 20 
                 0.5 
                 C 
                 PC3 
                 714.2005 
                 5237.471 
                 603.5695 
                 701.597 
                 579.7628 
                 0 
                 1992.76 
                 145.6409 
               
               
                 PC3_PSI_9 
                 20 
                 2 
                 C 
                 PC3 
                 977.4915 
                 2647.156 
                 907.0746 
                 696.3453 
                 780.8457 
                 1.564821 
                 1726.519 
                 103.2782 
               
               
                 PC3_PSI_12 
                 20 
                 5 
                 C 
                 PC3 
                 808.5563 
                 2683.619 
                 886.9759 
                 740.1477 
                 674.075 
                 1.668502 
                 1572.063 
                 123.4692 
               
               
                 PANC10_1 
                 1 
                 2 
                 A 
                 PANC10 
                 172.3212 
                 2480.381 
                 511.7418 
                 224.5398 
                 443.8577 
                 5.221856 
                 454.3014 
                 208.8742 
               
               
                 LNCaP_19 
                 1 
                 2 
                 A 
                 LNCaP 
                 333.6408 
                 2291.777 
                 799.8637 
                 627.944 
                 872.7111 
                 39.33756 
                 335.0977 
                 237.4823 
               
               
                 22RV1_1 
                 1 
                 2 
                 A 
                 22RV1 
                 204.5698 
                 1570.753 
                 726.7236 
                 340.4729 
                 436.3203 
                 178.8198 
                 535.0288 
                 213.1532 
               
               
                 PC3_1 
                 1 
                 2 
                 A 
                 PC3 
                 359.1507 
                 2068.708 
                 656.0487 
                 511.5903 
                 374.3149 
                 8.779241 
                 1169.235 
                 104.5528 
               
               
                 DU145_1 
                 1 
                 2 
                 A 
                 DU145 
                 428.5803 
                 2791.801 
                 1272.588 
                 303.6234 
                 601.7662 
                 29.59506 
                 2236.071 
                 213.7421 
               
               
                 PANC10_2 
                 1 
                 2 
                 A 
                 PANC10 
                 360.7956 
                 2703.702 
                 559.057 
                 205.8095 
                 464.4551 
                 7.548024 
                 968.6631 
                 170.5854 
               
               
                 22RV1_4 
                 1 
                 2 
                 A 
                 22RV1 
                 275.6773 
                 1360.843 
                 883.0026 
                 392.6313 
                 466.1452 
                 405.9974 
                 636.5639 
                 230.5664 
               
               
                 PC3_4 
                 1 
                 2 
                 A 
                 PC3 
                 458.1792 
                 2178.244 
                 964.6375 
                 547.1644 
                 436.4062 
                 9.466512 
                 1539.255 
                 96.55842 
               
               
                 DU145_4 
                 1 
                 2 
                 A 
                 DU145 
                 425.2251 
                 2922.966 
                 1519.357 
                 318.005 
                 674.9992 
                 13.40251 
                 2932.713 
                 191.2904 
               
               
                 PANC10_3 
                 1 
                 2 
                 A 
                 PANC10 
                 291.7501 
                 2341.482 
                 504.2041 
                 230.4078 
                 529.6387 
                 10.47308 
                 782.4888 
                 227.4155 
               
               
                 LNCaP_21 
                 1 
                 2 
                 A 
                 LNCaP 
                 324.7466 
                 3035.675 
                 943.1772 
                 570.4245 
                 827.398 
                 45.18214 
                 299.3317 
                 242.854 
               
               
                 22RV1_7 
                 1 
                 2 
                 A 
                 22RV1 
                 311.9075 
                 1321.287 
                 918.4447 
                 410.1174 
                 428.3044 
                 321.9103 
                 773.8578 
                 233.7033 
               
               
                 PC3_7 
                 1 
                 2 
                 A 
                 PC3 
                 497.9105 
                 2513.322 
                 1117.171 
                 449.1203 
                 439.7376 
                 8.75722 
                 1715.164 
                 113.2183 
               
               
                 DU145_7 
                 1 
                 2 
                 A 
                 DU145 
                 463.4813 
                 3105.64 
                 1746.725 
                 318.4463 
                 838.6805 
                 34.68227 
                 2785.092 
                 194.4309 
               
               
                 PANC10_10 
                 1 
                 5 
                 B 
                 PANC10 
                 290.1422 
                 2337.876 
                 388.3442 
                 206.4473 
                 508.8648 
                 6.695589 
                 594.7915 
                 208.6792 
               
               
                 LNCaP_3 
                 1 
                 5 
                 B 
                 LNCaP 
                 307.0182 
                 3542.893 
                 738.3057 
                 575.05 
                 833.3352 
                 20.71155 
                 213.2071 
                 202.2422 
               
               
                 22RV1_10 
                 1 
                 5 
                 B 
                 22RV1 
                 256.2929 
                 2413.425 
                 754.2656 
                 301.2566 
                 372.0744 
                 68.5696 
                 548.5568 
                 291.1398 
               
               
                 DU145_10 
                 1 
                 5 
                 B 
                 DU145 
                 515.1305 
                 4297.318 
                 1125.244 
                 215.636 
                 508.2849 
                 12.83548 
                 1396.5 
                 266.1222 
               
               
                 PANC10_13 
                 1 
                 5 
                 B 
                 PANC10 
                 285.2892 
                 2433.135 
                 415.2139 
                 297.1006 
                 530.6016 
                 9.994208 
                 551.4986 
                 233.501 
               
               
                 LNCaP_6 
                 1 
                 5 
                 B 
                 LNCaP 
                 315.4253 
                 3247.309 
                 814.5465 
                 559.5475 
                 821.7977 
                 18.12789 
                 154.6913 
                 212.7006 
               
               
                 22RV1_13 
                 1 
                 5 
                 B 
                 22RV1 
                 274.3347 
                 1939.19 
                 723.5315 
                 313.0766 
                 395.7958 
                 56.54226 
                 475.3738 
                 288.9938 
               
               
                 PC3_13 
                 1 
                 5 
                 B 
                 PC3 
                 501.935 
                 2271.85 
                 918.1279 
                 490.6696 
                 371.1315 
                 5.632687 
                 2335.062 
                 105.7693 
               
               
                 DU145_13 
                 1 
                 5 
                 B 
                 DU145 
                 502.594 
                 4769.716 
                 878.7183 
                 292.3586 
                 512.4488 
                 16.42464 
                 962.484 
                 275.934 
               
               
                 PANC10_16 
                 1 
                 5 
                 B 
                 PANC10 
                 252.0552 
                 2705.605 
                 456.2518 
                 292.4691 
                 539.2067 
                 13.82581 
                 727.4504 
                 201.006 
               
               
                 LNCaP_9 
                 1 
                 5 
                 B 
                 LNCaP 
                 374.2691 
                 3716.841 
                 777.7604 
                 548.4784 
                 873.2946 
                 10.11538 
                 149.4829 
                 173.0854 
               
               
                 22RV1_16 
                 1 
                 5 
                 B 
                 22RV1 
                 283.6185 
                 1800.302 
                 791.4308 
                 341.0175 
                 415.2986 
                 94.53951 
                 576.691 
                 269.4376 
               
               
                 PC3_16 
                 1 
                 5 
                 B 
                 PC3 
                 479.2463 
                 2269.692 
                 984.8311 
                 472.3754 
                 391.0696 
                 5.725762 
                 1983.977 
                 113.3701 
               
               
                 DU145_16 
                 1 
                 5 
                 B 
                 DU145 
                 671.7751 
                 1577.681 
                 628.551 
                 297.1659 
                 515.0876 
                 9.005028 
                 394.4202 
                 300.7679 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 16 
               
             
            
               
                   
               
               
                 Pressure-Sensitive Candidates 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 O2 
                 PSI 
                 Expt 
                 CellLine 
                 CXorf38 
                 PPID 
                 HIST1H2AG 
                 AC112229.1 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 LNCaP_25 
                 20 
                 0 
                 A 
                 LNCaP 
                 157.323 
                 796.7649 
                 159.0146 
                 16.91645 
               
               
                 22RV1_3 
                 20 
                 0 
                 A 
                 22RV1 
                 300.0304 
                 661.6742 
                 45.54033 
                 8.036528 
               
               
                 PC3_3 
                 20 
                 0 
                 A 
                 PC3 
                 409.9796 
                 470.0478 
                 39.65664 
                 2.915929 
               
               
                 DU145_3 
                 20 
                 0 
                 A 
                 DU145 
                 150.9284 
                 185.9263 
                 76.55789 
                 12.03053 
               
               
                 PANC10_8 
                 20 
                 0 
                 A 
                 PANC10 
                 441.2033 
                 994.5255 
                 42.42339 
                 67.87743 
               
               
                 LNCaP_26 
                 20 
                 0 
                 A 
                 LNCaP 
                 162.3936 
                 915.9727 
                 149.621 
                 12.77253 
               
               
                 22RV1_6 
                 20 
                 0 
                 A 
                 22RV1 
                 344.0181 
                 634.6364 
                 44.15755 
                 17.45764 
               
               
                 PC3_6 
                 20 
                 0 
                 A 
                 PC3 
                 330.5891 
                 599.0099 
                 70.945 
                 1.462784 
               
               
                 DU145_6 
                 20 
                 0 
                 A 
                 DU145 
                 146.1939 
                 177.2745 
                 65.61457 
                 11.51133 
               
               
                 PANC10_9 
                 20 
                 0 
                 A 
                 PANC10 
                 370.1235 
                 1038.943 
                 35.71367 
                 81.16744 
               
               
                 LNCaP_27 
                 20 
                 0 
                 A 
                 LNCaP 
                 183.4312 
                 896.9986 
                 165.2896 
                 18.14155 
               
               
                 22RV1_9 
                 20 
                 0 
                 A 
                 22RV1 
                 283.271 
                 607.5958 
                 45.15914 
                 14.36882 
               
               
                 PC3_9 
                 20 
                 0 
                 A 
                 PC3 
                 358.2714 
                 525.0916 
                 66.61609 
                 2.798996 
               
               
                 DU145_9 
                 20 
                 0 
                 A 
                 DU145 
                 127.6099 
                 254.1198 
                 111.1086 
                 9.90077 
               
               
                 PC3_PSI_1 
                 20 
                 0 
                 A 
                 PC3 
                 346.5858 
                 366.7281 
                 66.67783 
                 0 
               
               
                 PANC10_12 
                 20 
                 0 
                 B 
                 PANC10 
                 362.7008 
                 715.6739 
                 119.5106 
                 31.96214 
               
               
                 LNCaP_1 
                 20 
                 0 
                 B 
                 LNCaP 
                 121.4664 
                 863.8873 
                 160.0632 
                 18.1632 
               
               
                 22RV1_12 
                 20 
                 0 
                 B 
                 22RV1 
                 310.9827 
                 687.9044 
                 60.59261 
                 5.346407 
               
               
                 DU145_12 
                 20 
                 0 
                 B 
                 DU145 
                 159.7228 
                 258.2752 
                 147.2622 
                 10.19507 
               
               
                 PANC10_15 
                 20 
                 0 
                 B 
                 PANC10 
                 363.0775 
                 1083.542 
                 50.07966 
                 71.70497 
               
               
                 LNCaP_4 
                 20 
                 0 
                 B 
                 LNCaP 
                 128.0651 
                 699.6397 
                 163.1145 
                 16.17664 
               
               
                 22RV1_15 
                 20 
                 0 
                 B 
                 22RV1 
                 297.3061 
                 620.931 
                 59.46121 
                 9.74774 
               
               
                 PC3_15 
                 20 
                 0 
                 B 
                 PC3 
                 318.9095 
                 532.0582 
                 42.84669 
                 2.711816 
               
               
                 DU145_15 
                 20 
                 0 
                 B 
                 DU145 
                 151.2211 
                 228.8479 
                 112.9117 
                 14.11397 
               
               
                 PANC10_18 
                 20 
                 0 
                 B 
                 PANC10 
                 350.1105 
                 1150.598 
                 93.69155 
                 37.80536 
               
               
                 LNCaP_7 
                 20 
                 0 
                 B 
                 LNCaP 
                 146.3887 
                 928.5225 
                 170.4383 
                 9.410701 
               
               
                 22RV1_18 
                 20 
                 0 
                 B 
                 22RV1 
                 294.0389 
                 608.622 
                 60.34859 
                 10.2721 
               
               
                 PC3_18 
                 20 
                 0 
                 B 
                 PC3 
                 255.0779 
                 515.5685 
                 71.7195 
                 2.706396 
               
               
                 DU145_18 
                 20 
                 0 
                 B 
                 DU145 
                 183.5598 
                 332.7586 
                 141.0607 
                 11.75506 
               
               
                 PC3_PSI_2 
                 20 
                 0 
                 B 
                 PC3 
                 334.4338 
                 421.1631 
                 76.21673 
                 0 
               
               
                 PANC10_21 
                 20 
                 0 
                 C 
                 PANC10 
                 352.8687 
                 1048.89 
                 70.22044 
                 51.67164 
               
               
                 LNCaP_10 
                 20 
                 0 
                 C 
                 LNCaP 
                 141.9078 
                 635.5872 
                 101.9338 
                 4.996755 
               
               
                 22RV1_21 
                 20 
                 0 
                 C 
                 22RV1 
                 353.8414 
                 587.7764 
                 62.3043 
                 4.702211 
               
               
                 PC3_25 
                 20 
                 0 
                 C 
                 PC3 
                 279.2825 
                 552.7811 
                 58.66586 
                 1.652559 
               
               
                 DU145_21 
                 20 
                 0 
                 C 
                 DU145 
                 148.4224 
                 277.6609 
                 101.9773 
                 15.14514 
               
               
                 PANC10_24 
                 20 
                 0 
                 C 
                 PANC10 
                 404.2433 
                 807.1225 
                 49.56414 
                 69.11697 
               
               
                 LNCaP_13 
                 20 
                 0 
                 C 
                 LNCaP 
                 131.7968 
                 669.5749 
                 147.0947 
                 9.414058 
               
               
                 22RV1_24 
                 20 
                 0 
                 C 
                 22RV1 
                 324.989 
                 683.743 
                 112.2689 
                 10.97365 
               
               
                 PC3_26 
                 20 
                 0 
                 C 
                 PC3 
                 354.1772 
                 461.9702 
                 37.72757 
                 3.849752 
               
               
                 DU145_24 
                 20 
                 0 
                 C 
                 DU145 
                 183.0756 
                 371.0115 
                 98.8284 
                 11.34096 
               
               
                 PANC10_27 
                 20 
                 0 
                 C 
                 PANC10 
                 298.3092 
                 756.0595 
                 30.85957 
                 39.43167 
               
               
                 LNCaP_16 
                 20 
                 0 
                 C 
                 LNCaP 
                 136.6037 
                 710.339 
                 103.0737 
                 3.725554 
               
               
                 22RV1_27 
                 20 
                 0 
                 C 
                 22RV1 
                 294.735 
                 653.2787 
                 100.2707 
                 10.12835 
               
               
                 PC3_27 
                 20 
                 0 
                 C 
                 PC3 
                 259.1797 
                 490.1449 
                 72.17662 
                 4.593058 
               
               
                 DU145_27 
                 20 
                 0 
                 C 
                 DU145 
                 110.6876 
                 221.3752 
                 221.3752 
                 0 
               
               
                 PC3_PSI_3 
                 20 
                 0 
                 C 
                 PC3 
                 331.9262 
                 322.3744 
                 66.86283 
                 0 
               
               
                 PANC10_4 
                 10 
                 2 
                 A 
                 PANC10 
                 600.6535 
                 778.9193 
                 39.74078 
                 38.60533 
               
               
                 PC3_2 
                 10 
                 2 
                 A 
                 PC3 
                 472.4409 
                 450.8336 
                 30.36703 
                 4.087869 
               
               
                 DU145_2 
                 10 
                 2 
                 A 
                 DU145 
                 275.8476 
                 124.076 
                 42.09722 
                 2.215643 
               
               
                 LNCaP_23 
                 10 
                 2 
                 A 
                 LNCaP 
                 162.1426 
                 550.2499 
                 81.0713 
                 10.34953 
               
               
                 22RV1_5 
                 10 
                 2 
                 A 
                 22RV1 
                 380.6666 
                 448.2042 
                 29.82181 
                 3.508448 
               
               
                 PC3_5 
                 10 
                 2 
                 A 
                 PC3 
                 372.9124 
                 481.5501 
                 50.85169 
                 0 
               
               
                 DU145_5 
                 10 
                 2 
                 A 
                 DU145 
                 246.4338 
                 149.6562 
                 30.92894 
                 0.997708 
               
               
                 PANC10_6 
                 10 
                 2 
                 A 
                 PANC10 
                 419.9716 
                 833.6437 
                 47.4568 
                 29.81799 
               
               
                 LNCaP_24 
                 10 
                 2 
                 A 
                 LNCaP 
                 157.1973 
                 672.0185 
                 58.94899 
                 2.619955 
               
               
                 22RV1_8 
                 10 
                 2 
                 A 
                 22RV1 
                 375.7008 
                 509.2833 
                 34.91361 
                 6.830923 
               
               
                 PC3_8 
                 10 
                 2 
                 A 
                 PC3 
                 401.836 
                 542.9999 
                 49.7282 
                 0 
               
               
                 DU145_8 
                 10 
                 2 
                 A 
                 DU145 
                 201.8757 
                 157.1249 
                 94.47386 
                 1.988923 
               
               
                 PC3_PSI_4 
                 20 
                 0.5 
                 A 
                 PC3 
                 328.605 
                 267.7096 
                 61.46981 
                 0 
               
               
                 PC3_PSI_7 
                 20 
                 2 
                 A 
                 PC3 
                 481.1912 
                 353.0527 
                 24.19397 
                 0 
               
               
                 PC3_PSI_10 
                 20 
                 5 
                 A 
                 PC3 
                 460.4279 
                 359.4367 
                 28.92019 
                 0 
               
               
                 PANC10_11 
                 10 
                 5 
                 B 
                 PANC10 
                 454.4556 
                 695.9139 
                 33.05132 
                 43.15033 
               
               
                 LNCaP_2 
                 10 
                 5 
                 B 
                 LNCaP 
                 182.9043 
                 536.0503 
                 63.31302 
                 14.06956 
               
               
                 22RV1_11 
                 10 
                 5 
                 B 
                 22RV1 
                 371.4595 
                 611.9419 
                 46.5219 
                 5.725772 
               
               
                 DU145_11 
                 10 
                 5 
                 B 
                 DU145 
                 228.0267 
                 211.5711 
                 117.5395 
                 9.403162 
               
               
                 PANC10_14 
                 10 
                 5 
                 B 
                 PANC10 
                 415.1836 
                 770.6951 
                 51.26762 
                 39.50128 
               
               
                 LNCaP_5 
                 10 
                 5 
                 B 
                 LNCaP 
                 153.5422 
                 827.0341 
                 89.85708 
                 5.234393 
               
               
                 22RV1_14 
                 10 
                 5 
                 B 
                 22RV1 
                 334.8949 
                 598.0267 
                 33.31863 
                 5.980267 
               
               
                 PC3_14 
                 10 
                 5 
                 B 
                 PC3 
                 423.3261 
                 421.4585 
                 32.99454 
                 3.112692 
               
               
                 DU145_14 
                 10 
                 5 
                 B 
                 DU145 
                 209.4351 
                 163.9578 
                 111.2998 
                 9.574176 
               
               
                 PANC10_17 
                 10 
                 5 
                 B 
                 PANC10 
                 421.9334 
                 943.679 
                 47.07053 
                 26.08728 
               
               
                 LNCaP_8 
                 10 
                 5 
                 B 
                 LNCaP 
                 146.3051 
                 621.7965 
                 86.69929 
                 10.83741 
               
               
                 22RV1_17 
                 10 
                 5 
                 B 
                 22RV1 
                 363.6682 
                 566 
                 31.73832 
                 6.61215 
               
               
                 PC3_17 
                 10 
                 5 
                 B 
                 PC3 
                 284.6894 
                 405.555 
                 58.97657 
                 0.728106 
               
               
                 DU145_17 
                 10 
                 5 
                 B 
                 DU145 
                 147.2062 
                 210.9224 
                 147.2062 
                 6.591324 
               
               
                 PC3_PSI_5 
                 20 
                 0.5 
                 B 
                 PC3 
                 335.8502 
                 326.6554 
                 55.16847 
                 0 
               
               
                 PC3_PSI_8 
                 20 
                 2 
                 B 
                 PC3 
                 469.4987 
                 316.845 
                 31.06324 
                 0 
               
               
                 PC3_PSI_11 
                 20 
                 5 
                 B 
                 PC3 
                 539.0108 
                 343.1889 
                 28.03176 
                 0.400454 
               
               
                 PANC10_19 
                 20 
                 2 
                 C 
                 PANC10 
                 483.0943 
                 533.8059 
                 40.03544 
                 20.01772 
               
               
                 PANC10_20 
                 20 
                 5 
                 C 
                 PANC10 
                 452.5607 
                 662.2389 
                 43.04246 
                 30.74461 
               
               
                 LNCaP_11 
                 20 
                 2 
                 C 
                 LNCaP 
                 240.1089 
                 422.4138 
                 59.28615 
                 4.446461 
               
               
                 LNCaP_12 
                 20 
                 5 
                 C 
                 LNCaP 
                 158.5188 
                 505.1699 
                 74.9045 
                 5.225895 
               
               
                 22RV1_19 
                 20 
                 2 
                 C 
                 22RV1 
                 442.0038 
                 444.5736 
                 50.1109 
                 2.56979 
               
               
                 PC3_19 
                 20 
                 5 
                 C 
                 PC3 
                 365.3888 
                 425.8157 
                 43.11164 
                 2.826993 
               
               
                 PC3_22 
                 20 
                 2 
                 C 
                 PC3 
                 442.2107 
                 392.7946 
                 48.14902 
                 3.167699 
               
               
                 DU145_19 
                 20 
                 2 
                 C 
                 DU145 
                 178.4925 
                 199.2475 
                 75.75556 
                 4.150989 
               
               
                 DU145_20 
                 20 
                 5 
                 C 
                 DU145 
                 176.0144 
                 205.6797 
                 74.16335 
                 0.988845 
               
               
                 PANC10_22 
                 20 
                 2 
                 C 
                 PANC10 
                 467.6097 
                 684.5547 
                 33.94302 
                 30.48172 
               
               
                 PANC10_23 
                 20 
                 5 
                 C 
                 PANC10 
                 519.5796 
                 717.4112 
                 30.97912 
                 13.58733 
               
               
                 LNCaP_14 
                 20 
                 2 
                 C 
                 LNCaP 
                 160.3208 
                 605.1844 
                 91.30852 
                 6.370362 
               
               
                 LNCaP_15 
                 20 
                 5 
                 C 
                 LNCaP 
                 130.5521 
                 777.2683 
                 126.9256 
                 6.044077 
               
               
                 22RV1_22 
                 20 
                 2 
                 C 
                 22RV1 
                 321.0163 
                 484.0324 
                 68.96835 
                 3.76191 
               
               
                 22RV1_23 
                 20 
                 5 
                 C 
                 22RV1 
                 349.8858 
                 559.3492 
                 65.53046 
                 1.170187 
               
               
                 PC3_20 
                 20 
                 5 
                 C 
                 PC3 
                 364.1117 
                 422.6891 
                 29.95435 
                 1.331304 
               
               
                 PC3_23 
                 20 
                 2 
                 C 
                 PC3 
                 389.304 
                 441.5039 
                 35.12517 
                 2.439248 
               
               
                 DU145_22 
                 20 
                 2 
                 C 
                 DU145 
                 191.9107 
                 206.0391 
                 70.64198 
                 9.418931 
               
               
                 DU145_23 
                 20 
                 5 
                 C 
                 DU145 
                 167.2201 
                 223.845 
                 98.20866 
                 11.50191 
               
               
                 PANC10_26 
                 20 
                 5 
                 C 
                 PANC10 
                 404.7071 
                 856.228 
                 55.93347 
                 14.99666 
               
               
                 LNCaP_17 
                 20 
                 2 
                 C 
                 LNCaP 
                 148.4778 
                 553.302 
                 60.91398 
                 7.614247 
               
               
                 LNCaP_18 
                 20 
                 5 
                 C 
                 LNCaP 
                 188.6932 
                 736.3874 
                 87.08918 
                 7.741261 
               
               
                 22RV1_25 
                 20 
                 2 
                 C 
                 22RV1 
                 365.117 
                 479.4294 
                 54.59694 
                 3.412309 
               
               
                 22RV1_26 
                 20 
                 5 
                 C 
                 22RV1 
                 346.6987 
                 537.7041 
                 64.20347 
                 1.605087 
               
               
                 PC3_21 
                 20 
                 5 
                 C 
                 PC3 
                 274.961 
                 448.2308 
                 56.76932 
                 0.493646 
               
               
                 PC3_24 
                 20 
                 2 
                 C 
                 PC3 
                 313.4012 
                 483.7704 
                 50.76904 
                 0.976328 
               
               
                 DU145_25 
                 20 
                 2 
                 C 
                 DU145 
                 164.7058 
                 204.7694 
                 73.14764 
                 4.451509 
               
               
                 DU145_26 
                 20 
                 5 
                 C 
                 DU145 
                 0 
                 241.1813 
                 0 
                 0 
               
               
                 PC3_PSI_6 
                 20 
                 0.5 
                 C 
                 PC3 
                 270.2759 
                 310.8873 
                 47.61337 
                 0 
               
               
                 PC3_PSI_9 
                 20 
                 2 
                 C 
                 PC3 
                 498.1347 
                 301.4888 
                 28.16678 
                 0 
               
               
                 PC3_PSI_12 
                 20 
                 5 
                 C 
                 PC3 
                 464.5111 
                 288.9846 
                 37.37445 
                 1.001101 
               
               
                 PANC10_1 
                 1 
                 2 
                 A 
                 PANC10 
                 579.626 
                 704.9505 
                 46.9967 
                 62.66227 
               
               
                 LNCaP_19 
                 1 
                 2 
                 A 
                 LNCaP 
                 166.0919 
                 463.309 
                 75.76123 
                 8.74168 
               
               
                 22RV1_1 
                 1 
                 2 
                 A 
                 22RV1 
                 376.2368 
                 414.8619 
                 38.62507 
                 2.861117 
               
               
                 PC3_1 
                 1 
                 2 
                 A 
                 PC3 
                 580.228 
                 418.2111 
                 35.11696 
                 3.990564 
               
               
                 DU145_1 
                 1 
                 2 
                 A 
                 DU145 
                 178.6665 
                 160.0325 
                 46.03676 
                 2.192227 
               
               
                 PANC10_2 
                 1 
                 2 
                 A 
                 PANC10 
                 587.7395 
                 823.2379 
                 30.1921 
                 19.12166 
               
               
                 22RV1_4 
                 1 
                 2 
                 A 
                 22RV1 
                 435.2359 
                 399.3143 
                 36.75697 
                 0.835386 
               
               
                 PC3_4 
                 1 
                 2 
                 A 
                 PC3 
                 383.3937 
                 441.1394 
                 46.38591 
                 0.946651 
               
               
                 DU145_4 
                 1 
                 2 
                 A 
                 DU145 
                 218.0954 
                 155.9565 
                 42.64435 
                 3.65523 
               
               
                 PANC10_3 
                 1 
                 2 
                 A 
                 PANC10 
                 408.4502 
                 715.1619 
                 91.26543 
                 19.45001 
               
               
                 LNCaP_21 
                 1 
                 2 
                 A 
                 LNCaP 
                 200.4958 
                 553.4812 
                 70.5971 
                 2.823884 
               
               
                 22RV1_7 
                 1 
                 2 
                 A 
                 22RV1 
                 361.0124 
                 435.5792 
                 35.4647 
                 1.818702 
               
               
                 PC3_7 
                 1 
                 2 
                 A 
                 PC3 
                 437.861 
                 455.3754 
                 41.28404 
                 1.251031 
               
               
                 DU145_7 
                 1 
                 2 
                 A 
                 DU145 
                 223.8583 
                 146.0859 
                 98.79193 
                 3.152934 
               
               
                 PANC10_10 
                 1 
                 5 
                 B 
                 PANC10 
                 398.3875 
                 639.4287 
                 20.08677 
                 49.10099 
               
               
                 LNCaP_3 
                 1 
                 5 
                 B 
                 LNCaP 
                 170.5657 
                 650.5862 
                 102.3394 
                 13.40159 
               
               
                 22RV1_10 
                 1 
                 5 
                 B 
                 22RV1 
                 338.3516 
                 611.506 
                 69.69369 
                 6.744551 
               
               
                 DU145_10 
                 1 
                 5 
                 B 
                 DU145 
                 158.3042 
                 209.6461 
                 89.84835 
                 8.556986 
               
               
                 PANC10_13 
                 1 
                 5 
                 B 
                 PANC10 
                 421.5739 
                 652.3492 
                 69.05089 
                 19.98842 
               
               
                 LNCaP_6 
                 1 
                 5 
                 B 
                 LNCaP 
                 160.734 
                 729.9497 
                 102.7247 
                 14.50231 
               
               
                 22RV1_13 
                 1 
                 5 
                 B 
                 22RV1 
                 362.2893 
                 560.1872 
                 41.88315 
                 3.141237 
               
               
                 PC3_13 
                 1 
                 5 
                 B 
                 PC3 
                 443.7305 
                 362.9954 
                 26.91172 
                 1.251708 
               
               
                 DU145_13 
                 1 
                 5 
                 B 
                 DU145 
                 225.0176 
                 197.0957 
                 80.48074 
                 4.927392 
               
               
                 PANC10_16 
                 1 
                 5 
                 B 
                 PANC10 
                 424.3461 
                 656.1943 
                 72.31963 
                 22.334 
               
               
                 LNCaP_9 
                 1 
                 5 
                 B 
                 LNCaP 
                 206.8033 
                 877.7903 
                 107.8974 
                 8.99145 
               
               
                 22RV1_16 
                 1 
                 5 
                 B 
                 22RV1 
                 360.6007 
                 596.2742 
                 47.26976 
                 2.701129 
               
               
                 PC3_16 
                 1 
                 5 
                 B 
                 PC3 
                 352.1344 
                 346.4086 
                 57.25762 
                 2.290305 
               
               
                 DU145_16 
                 1 
                 5 
                 B 
                 DU145 
                 203.5136 
                 72.04023 
                 102.6573 
                 0 
               
               
                   
               
            
           
         
       
     
     Example 7: Primary Tumor Culture 
     To culture primary tumors using a method disclosed herein, target cells are isolated from a patient tumor. The cells are enriched for, for example, T-cells, dendritic cells, macrophages, B-cells, neutrophils, cancer cells, cancer stem cells, fibroblasts, and endothelial cells. The isolated cells are then co-cultured to re-establish tumor heterogeneity. To replicate the metastatic microenvironment, the cells are grown under low oxygen and high pressure conditions in an ex vivo setting. The cells are then subcutaneously injected into mice and downstream molecular assays are performed to determine gene expression changes. 
       FIGS. 50-52  shows ex vivo cultures of pancreatic ductal adenocarcinoma colonies from a fine-needle aspirate. The cells were stained for DAPI. The cancerous cells were further stained for EpCAM (around periphery of cell) and CK7. The cells that did not get labeled with either CK7 or EpCAM represent the stromal cells derived from the biopsy. 
       FIG. 42  shows the mutations found using the COSMIC database from pancreatic ductal adenocarcinoma (PDAC) and circulating tumor cells (CTC) cultured under low oxygen (1% O 2 ) and positive pressure (2 PSI) using whole exome sequencing (top panels). The bottom panels of  FIG. 42  show that the NANOG and Wnt signaling pathways exhibited increased gene expression in both PDAC and CTC colonies as determined by mRNA sequencing of the cells. 
       FIG. 43  shows that there was increased ex vivo expansion of primary cells. The individual lines represent patient PBMC populations from cryopreserved blood samples. The viability of individual patient PBMC populations was tracked following transfection and subsequent recovery and expansion under low oxygen and positive pressure conditions.  FIG. 44  shows that there was increased ex vivo expansion of primary cells. The individual lines represent patient PBMC populations from cryopreserved blood samples. The viability of individual patient PBMC populations was tracked following transfection and subsequent recovery and expansion under low oxygen and positive pressure conditions. 
       FIG. 46  shows the results of the ex vivo culture and expansion of tumor-infiltrating lymphocytes (TILs) enriched from renal cell carcinoma tumors using positive pressure and low oxygen conditions. The experiments were performed in duplicate; RCC1 and RCC2 indicate the two different cell populations analyzed. For each data point, the left bar is RCC1 and the right bar is RCC2. The results indicate that 1% O 2  and 2 PSI maintained the immune cell viability of CD3+, CD4+, CD8+, and CD11b+ cell types, as indicated by FACS analysis. The two different tumors were additionally cultured in two different culture media formulations: Media A and Media B. Media A was supplemented with 10% fetal calf serum, while Media B was animal-component free, chemically defined, and composed of recombinant human growth factors. 
     Example 8: Three-Dimensional Cell Culturing Using a Method Disclosed Herein 
       FIG. 39  shows a biopsy culture taken from a patient having prostate cancer. The cells were cultured for 48 hours under either 21% oxygen and 0 PSI or 1% oxygen and 2 PSI. The results indicated that the cells had a two-fold increase in viable cell adherence under positive pressure and low oxygen conditions. The bottom panels of  FIG. 39  show that only cells grown under high pressure and low oxygen yielded enough cells for passaging at day 6, and were then able to form organoids by day 10. The tumor cell cultures were then subcutaneously injected into mice. 
       FIG. 40  shows prostate cancer cells obtained from another patient could form organoids after two weeks of culture under high pressure and low oxygen conditions. 
       FIG. 41  shows an apheresis culture taken from a patient having prostate cancer. The cells were cultured for 10 days under high pressure and low oxygen conditions to form organoids  FIG. 41  shows the results of culturing under both 2D and 3D conditions. The left panels of  FIG. 41  show viable and proliferating tumor cells that were positively selected for EpCAM (prostate cancer marker), and the right panels show viable and proliferating tumor cells that are EpCAM negative. After ten days in culture, the cells were subcutaneously injected into mice. 
     EMBODIMENTS 
     The following non-limiting embodiments provide illustrative examples of the invention, but do not limit the scope of the invention. 
     Embodiment 1 
     A method for increasing transfection efficiency of a nucleic acid that is introduced into a cell, the method comprising culturing the cell in a hypoxic condition and a positive pressure condition, wherein culturing the cell in the hypoxic condition and the positive pressure condition increases expression of a polypeptide encoded by the nucleic acid that is introduced into the cell as compared to expression of the polypeptide encoded by a nucleic acid that is introduced into a cell that is cultured in the absence of the hypoxic condition and the positive pressure condition. 
     Embodiment 2 
     The method of embodiment 1, wherein the cell is cultured in a culture medium that does not contain serum. 
     Embodiment 3 
     The method of any one of embodiments 1-2, wherein the cell is contacted with a substrate. 
     Embodiment 4 
     The method of any one of embodiments 1-3, wherein the substrate does not contain serum. 
     Embodiment 5 
     The method of any one of embodiments 1-4, wherein the hypoxic condition comprises an oxygen level of about 2%. 
     Embodiment 6 
     The method of any one of embodiments 1-4, wherein the hypoxic condition comprises an oxygen level of about 5%. 
     Embodiment 7 
     The method of any one of embodiments 1-6, wherein the positive pressure condition comprises a pressure level from about 2 PSI to about 10 PSI. 
     Embodiment 8 
     The method of any one of embodiments 1-7, wherein the nucleic acid is DNA. 
     Embodiment 9 
     The method of any one of embodiments 1-7, wherein the nucleic acid is RNA. 
     Embodiment 10 
     The method of any one of embodiments 1-7, wherein the nucleic acid is circular DNA. 
     Embodiment 11 
     The method of any one of embodiments 1-7, wherein the nucleic acid is supercoiled DNA. 
     Embodiment 12 
     The method of any one of embodiments 1-11, wherein the nucleic acid that is introduced into the cell is introduced via electroporation of the cell. 
     Embodiment 13 
     The method of any one of embodiments 1-11, wherein the nucleic acid that is introduced into the cell is introduced via encapsulation of the nucleic acid in a cationic liposome. 
     Embodiment 14 
     The method of any one of embodiments 1-13, wherein culturing the cell in the hypoxic condition and the positive pressure condition increases an entry rate of the nucleic acid into the cell as compared to the entry rate of the nucleic acid that is introduced into the cell that is cultured in the absence of the hypoxic condition and the positive pressure condition. 
     Embodiment 15 
     The method of any one of embodiments 1-14, wherein the positive pressure condition is applied continuously to the cell. 
     Embodiment 16 
     The method of any one of embodiments 1-14, wherein the positive pressure condition is applied in pulses of positive pressure to the cell. 
     Embodiment 17 
     The method of any one of embodiments 1-16, wherein the culturing of the cell in the hypoxic condition and the positive pressure condition occurs after the nucleic acid is introduced into the cell. 
     Embodiment 18 
     The method of any one of embodiments 1-16, wherein the culturing of the cell in the hypoxic condition and the positive pressure condition occurs before the nucleic acid is introduced into the cell. 
     Embodiment 19 
     The method of any one of embodiments 1-16, wherein the culturing of the cell in the hypoxic condition and the positive pressure condition occurs before the nucleic acid is introduced into the cell and after the nucleic acid is introduced into the cell. 
     Embodiment 20 
     The method of any one of embodiments 1-19, wherein the nucleic acid is introduced into the cell in the absence of the hypoxic condition and the positive pressure condition. 
     Embodiment 21 
     The method of any one of embodiments 1-20, wherein the cell is a mammalian cell. 
     Embodiment 22 
     A method for reprogramming a cell, the method comprising culturing the cell in a hypoxic condition and a positive pressure condition, wherein the cell exhibits a rate of reprogramming that is higher than the rate of reprogramming of a cell cultured in the absence of the hypoxic condition and the positive pressure condition. 
     Embodiment 23 
     The method of embodiment 22, wherein the hypoxic condition comprises an oxygen level of about 2%. 
     Embodiment 24 
     The method of embodiment 22, wherein the hypoxic condition comprises an oxygen level of about 5%. 
     Embodiment 25 
     The method of any one of embodiments 22-24, wherein the positive pressure condition comprises a pressure level of about 2 PSI to about 10 PSI. 
     Embodiment 26 
     The method of any one of embodiments 22-25, wherein the rate of reprogramming of the cell cultured in the hypoxic condition and the positive pressure condition is about 10% higher than the rate of reprogramming of the cell cultured in the absence of the hypoxic condition and the positive pressure condition. 
     Embodiment 27 
     The method of any one of embodiments 22-25, wherein the rate of reprogramming of the cell cultured in the hypoxic condition and the positive pressure condition is about 20% higher than the rate of reprogramming of the cell cultured in the absence of the hypoxic condition and the positive pressure condition. 
     Embodiment 28 
     The method of any one of embodiments 22-27, wherein the cell is a somatic cell. 
     Embodiment 29 
     The method of any one of embodiments 22-27, wherein the cell is a fibroblast. 
     Embodiment 30 
     The method of any one of embodiments 22-29, wherein the cell is reprogrammed into a stem cell. 
     Embodiment 31 
     The method of any one of embodiments 22-30, wherein the cell is reprogrammed into a pluripotent stem cell. 
     Embodiment 32 
     The method of any one of embodiments 22-29, wherein the cell is reprogrammed into an immune cell. 
     Embodiment 33 
     The method of any one of embodiments 22-32, wherein the cell cultured in the hypoxic condition and the positive pressure condition exhibits a greater expression level of a stem cell marker as compared to the expression level of the stem cell marker for a cell cultured in the absence of the hypoxic condition and the positive pressure condition. 
     Embodiment 34 
     The method of embodiment 33, wherein the stem cell marker is Oct4. 
     Embodiment 35 
     The method of embodiment 33, wherein the stem cell marker is Nanog. 
     Embodiment 36 
     The method of embodiment 33, wherein the stem cell marker is Sox2. 
     Embodiment 37 
     The method of any one of embodiments 22-36, wherein the cell is contacted with a substrate. 
     Embodiment 38 
     The method of any one of embodiments 22-37, wherein a nucleic acid encoding a reprogramming factor polypeptide is introduced into the cell.