Patent Publication Number: US-2023146455-A1

Title: Treatment of inflammatory diseases with peptides and pharmaceutical compositions

Description:
CROSS-REFERENCE TO A RELATED APPLICATION 
     This application claims the benefit of U.S. Provisional Application No. 62/985,603, filed on Mar. 5, 2020, which application is hereby incorporated by reference in its entirety. 
    
    
     TECHNICAL FIELD 
     The present invention relates to treating inflammatory diseases with peptides and pharmaceutical compositions. 
     BACKGROUND 
     Inflammation is a protective mechanism in mammals from invading pathogens. However uncontrolled inflammation can cause tissue damage and is the cause of many diseases. Inflammatory diseases, either chronic or acute in nature, afflict many patients every day and present an important problem in the health care industry. Diseases and disorders which have significant inflammatory components include skin disorders, bowel disorders, certain degenerative neurological disorders, arthritis, and autoimmune diseases. For some patients, dietary or environmental factors may trigger an autoimmune or inflammatory response. In other patients, genetic factors can play a key part in disease. In many inflammatory conditions, pro-inflammatory cytokines, particularly IL-1β, and IL-6, play an important role in the pathogenesis of various inflammation-related diseases. 
     Interleukin-6 (IL-6) is a major pro-inflammatory cytokine and consists of 212 amino acids with two N-linked glycosylation sites. The IL-6 glycoprotein has a molecular weight of about 26 kDa. IL-6 signaling is mediated by the binding of IL-6 to either soluble or surface bound IL-6 receptor chain (IL-6R), enabling interaction of the complex with the cell surface transmembrane gp130 subunit. The interaction mediates intracellular signaling and is responsible for the proliferation and differentiation of immune cells. 
     Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine that is produced as a precursor by activated macrophages. The molecular weight of the proteolytically processed IL-1β is 17.5 kDa. Upon proteolytic cleavage, signal transduction is initiated by binding of active IL-1β to IL-1 receptor type I (IL-1R1), which in turn associates with the transmembrane IL-1 receptor accessory protein (IL-1RAP). The formed complex triggers signal transduction. IL-1β is key mediator in the inflammatory response and the cytokine affects a number of cellular activities such as cell proliferation, differentiation, and apoptosis. 
     New therapies for reducing inflammation are needed. 
     SUMMARY OF INVENTION 
     In one aspect, provided herein are methods of preventing or treating an IL-1α, IL-1β, IL-2, IL-6, IL-8, IL-10, TNF-α, MMP3, CCL-2, CCL-3, CCL-4, Fas, TIMP-1, or a Th1, Th2, and/or Th17 derived cytokine or proinflammatory chemokine mediated disease or disorder in a subject, comprising administering to the subject a compound disclosed herein. 
     In a further aspect, provided are methods of reducing production of IL-1α, IL-1β, IL-2, IL-6, IL-8, IL-10, TNF-α, MMP3, CCL-2, CCL-3, CCL-4, Fas, TIMP-1, or a Th1, Th2, and/or Th17 derived cytokine or proinflammatory chemokine in cells of a subject, comprising administering to the subject a compound disclosed herein. 
     In yet another aspect, provided are methods of reducing NF-κB transcription activity in cells of a subject, comprising administering to the subject a compound disclosed herein. 
     In another aspect, provided are methods of preventing or treating Keratoconjunctivitis sicca (Dry Eye) in a subject, comprising administering to the subject a compound disclosed herein. 
     In yet another aspect, provided are methods of preventing or treating Dry Eye Disease (DED) in a subject, comprising administering to the subject a compound disclosed herein. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG.  1 A- 1 D  are diagrams showing attenuation of IL-6 release of poly I:C stimulated primary human corneal epithelial cells by test compounds in a dose dependent manner; Compounds tested: YDE-093, YDE-096, YDE-100, YDE-101, YDE-102, YDE-103, YDE-104, YDE-105, YDE-106, and YDE-107. 
         FIG.  2 A- 2 BF  are diagrams showing an effect on cell proliferation of primary corneal epithelial cells after 48 hrs and 72 hrs with the following test compounds: YDE-012, YDE-019, YDE-038, YDE-044, YDE-045, YDE-047, YDE-048, YDE-049, YDE-050, YDE-051, YDE-052, YDE-053, YDE-054, YDE-055, YDE-056, YDE-057, YDE-058, YDE-059, YDE-060, YDE-061, YDE-062, YDE-063, YDE-064, YDE-065, YDE-066, YDE-067, YDE-072, YDE-073, YDE-074, YDE-075, YDE-076, YDE-077, YDE-078, YDE-079, YDE-080, YDE-081, YDE-082, YDE-083, YDE-084, YDE-085, YDE-086, YDE-087, Diquas, and hEGF. 
         FIG.  3    is a diagram showing the effect of YDE-053, YDE-060, or YDE-065 on the IL-1beta, IL-6, IL-8, MIP-1 alpha, MIP-1 beta, RANTES, and TNF-alpha release of poly I:C stimulated primary human corneal epithelial cells. 
         FIG.  4    is a diagram showing the effect of YDE-053 on NF-κB (p65) transcription activity of poly I:C stimulated primary human corneal cells. 
         FIG.  5    is a diagram representing the effect of test compounds on IL-6 release of poly I:C stimulated primary human corneal epithelial cells; Compounds tested: YDE-053, YDE-048, YDE-056, YDE-057, YDE-058, YDE-067, YDE-079, YDE-011, YDE-093, YDE-096, YDE-053, and YDE-043. 
         FIG.  6    is a diagram showing the effect of various stimulants on the level of IL-6. 
         FIG.  7    is a diagram showing the effect of Xiidra® on the level of IL-6 induced by various stimulants. 
         FIG.  8    is a diagram showing the effect of YDE-011 on the level of IL-6 induced by various stimulants. 
         FIG.  9    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-2 induced by LPS. 
         FIG.  10    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-6 induced by LPS. 
         FIG.  11    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-8 induced by LPS. 
         FIG.  12    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-10 induced by LPS. 
         FIG.  13    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of TNF-α induced by LPS. 
         FIG.  14    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of MMP-3 induced by LPS. 
         FIG.  15    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of CCL2/MCP-1 induced by LPS. 
         FIG.  16    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of CCL3/MIP-1α induced by LPS. 
         FIG.  17    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of CCL4/MIP-1β induced by LPS. 
         FIG.  18    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-1α/IL-1F1 induced by LPS. 
         FIG.  19    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-1β/IL-1F2 induced by LPS. 
         FIG.  20    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of TIMP-1 induced by LPS. 
         FIG.  21    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of Leptin induced by LPS. 
         FIG.  22    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of MMP-9 induced by LPS. 
         FIG.  23    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-10 induced by LPS. 
         FIG.  24    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-8 induced by LPS. 
         FIG.  25    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-6 induced by LPS. 
         FIG.  26    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of TNF-α induced by LPS. 
         FIG.  27    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of CCL3/MIP-1α induced by LPS. 
         FIG.  28    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of CCL4/MIP-1β induced by LPS. 
         FIG.  29    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of CCL5/RANTES induced by LPS. 
         FIG.  30    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IFN-γ induced by LPS. 
         FIG.  31    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of CCL2/MCP-1 induced by LPS. 
         FIG.  32    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of Fas Ligand induced by LPS. 
         FIG.  33    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of CCL20/MIP-3a induced by LPS. 
         FIG.  34    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-1α induced by LPS. 
         FIG.  35    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-1β/IL-1F2 induced by LPS. 
         FIG.  36    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of TIMP-1 induced by LPS. 
         FIG.  37    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of GM-CSF induced by LPS. 
         FIG.  38    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-2 induced by LPS. 
         FIG.  39    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-17A induced by LPS. 
         FIG.  40    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of IL-4 induced by LPS. 
         FIG.  41    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of VEGF-A induced by LPS. 
         FIG.  42    is a diagram showing the effect of YY-101, YDE-011, and YDE-043 on the level of MMP-9 induced by LPS. 
         FIG.  43    is a diagram showing the effect of YY-101, YDE-011, YDE-043, YDE-048, and YDE-060 on the level of Eotaxin &amp; Gro-α_KC in rat tear. 
         FIG.  44    is a diagram showing the effect of YY-101, YDE-011, YDE-043, YDE-048, and YDE-060 on the level of IL-17A &amp; IL-1β in rat tear. 
         FIG.  45    is a diagram showing the effect of YY-101, YDE-011, YDE-043, YDE-048, and YDE-060 on the level of IL-21 &amp; IL-4 in rat tear. 
         FIG.  46    is a diagram showing the effect of YY-101, YDE-011, YDE-043, YDE-048, and YDE-060 on the level of MCP-1 &amp; MCP-3 in rat tear. 
         FIG.  47    is a diagram showing the effect of YY-101, YDE-011, YDE-043, YDE-048, and YDE-060 on the level of sVCAM-1 &amp; TNF-α in rat tear. 
         FIG.  48    is a diagram showing the effect of YY-101, YDE-011, YDE-043, YDE-048, and YDE-060 on the level of IL-12p70 &amp; VEGF-A in rat tear. 
         FIG.  49    is a diagram showing the effect of YY-101, YDE-011, YDE-043, YDE-048, and YDE-060 on the level of IL-1α &amp; IP-10 in rat tear. 
         FIG.  50    is a diagram showing the effect of YY-101, YDE-011, YDE-043, YDE-048, and YDE-060 on the level of bNGF &amp; Leptin in rat tear. 
         FIG.  51    is a diagram showing the effect of YY-101, YDE-011, YDE-043, YDE-048, and YDE-060 on the level of RANTES in rat tear. 
         FIG.  52    is a diagram showing the effect of YDE-048 and YDE-043 on the level of Gro-α_KC &amp; IL-1α in rat goblet cell. 
         FIG.  53    is a diagram showing the effect of YDE-048 and YDE-043 on the level of IL-1β &amp; Leptin in rat goblet cell. 
         FIG.  54    is a diagram showing the effect of YDE-048 and YDE-043 on the level of IP-10 &amp; VEGF-A in rat goblet cell. 
         FIG.  55    is a diagram showing the effect of YDE-048 and YDE-043 on the level of Eotaxin &amp; IL-17A in rat goblet cell. 
         FIG.  56    is a diagram showing the effect of YDE-048 and YDE-043 on the level of MCP-1 &amp; MCP-3 in rat goblet cell. 
         FIG.  57    is a diagram showing the effect of YDE-048 and YDE-043 on the level of RANTES &amp; sVCAM-1 in rat goblet cell. 
         FIG.  58    is a diagram showing the effect of YDE-048 and YDE-043 on the level of TGF-β &amp; TNF-α in rat goblet cell. 
         FIG.  59    is a diagram showing the effect of YDE-048 and YDE-043 on the level of IL-12p70, IL-21, bNGF in rat goblet cell. 
         FIG.  60    is a diagram showing the effect of YDE-048 and YDE-043 on the level of IL-4 in rat goblet cell. 
         FIG.  61    is a diagram showing the effect of YDE-048 and YDE-043 on the level of IL-6 in rat goblet cell. 
         FIG.  62    shows an experimental schematic timeline of Mouse IBD Model in Example 12. 
         FIG.  63    shows body weight &amp; BW change over time, disease activity index. a: found dead (G4; n=3); b: animals sacrificed moribund (G2; n=3, G3; n=1, G4; n=1, G5; n=1); a: found dead (G4; n=3); b: animals sacrificed moribund (G2; n=3, G3; n=1, G4; n=1, G5; n=1). 
         FIG.  64    shows clinical scores (individual scores of DAI). a: found dead (G4; n=3); b: animals sacrificed moribund (G2; n=3, G3; n=1, G4; n=1, G5; n=1); All values were presented as mean±standard error (SEM); All values were statistically analyzed by one-way ANOVA with LSD post-hoc analysis; *: 0.05&gt;p, **: 0.01&gt;p, ***: 0.01&gt;p; G2 vs G3, G4, G5. 
         FIG.  65    shows CRP Levels (C-Reactive Protein) from Plasma. G1; n=8, G2; n=8, G3; n=8, G4; n=5, G5; n=8. Statistical analysis. All values were presented as mean±standard error (SEM). All values were statistically analyzed by one-way ANOVA with LSD post-hoc analysis. *: 0.05&gt;p, **: 0.01&gt;p, ***: 0.001&gt;p; G2 vs G3, G4, G5. #: 0.05&gt;p, ##: 0.01&gt;p, ###: 0.001&gt;p; G1 vs G2, G3, G4, G5  FIG.  66    shows study design of Mouse CIA-induced Rheumatoid Arthritis Model. 
         FIG.  67    shows Body weight &amp; Hind Paw Thickness (Inflammation). 
         FIG.  68    shows overall clinical symptom &amp; score. Clinical score: 0—no change; 1—swelling and erythema of the digit; 2—mild swelling and erythema of the limb; 3—gross swelling and erythema of the digit; 4—gross deformity and inability to use the limb. 
         FIG.  69    shows a study scheme &amp; group information of OVX induced Osteoporosis disease model in C57bL6 mouse. 
         FIG.  70    shows Body weight and Uterus Weight &amp; Gross Observation. G1; n=7, G2; n=7, G3; n=7, G4; n=7. Statistical analysis: All values were presented as mean±standard error (SEM). All values were statistically analyzed by one-way ANOVA with LSD post-hoc analysis. *: 0.05&gt;p, **: 0.01&gt;p, ***: 0.001&gt;p; G1 vs G2, G3, G4. #: 0.05&gt;p, ##: 0.01&gt;p, ###: 0.001&gt;p; G2 vs G3, G4. 
         FIG.  71    shows results of Lumbar Vertebrae Bone Strength Test. 0.75 kgf/1 cm; 0.75*9.8 N*x cm=Force (N*cm). Top-right image shows device used for compress test. Middle-right image shows total weight. Bottom-right image shows sample before test. 
         FIG.  72    shows Micro CT Parameters. 
         FIG.  73    shows results of Micro CT: Trabecular Area Analysis. BMD: Bone Mineral Density; Tb.N: Trabecular Number; Tb.Th: Trabecular Thickness; Tb. Sp: Trabecular Separation; Conn.D: Connectivity density. TV: Total volume; BV: Trabecular Bone Volume; BS: Bone Separation; BV/TV: Bone Volume/Total Volume; BS/TV: Bone Separation/Total Volume; BS/BV: Bone Separation/Bone Volume. G1; n=7, G2; n=7, G3; n=7, G4; n=7. Statistical analysis: All values were presented as mean±standard error (SEM). All values were statistically analyzed by one-way ANOVA with LSD post-hoc analysis. *: 0.05&gt;p, **: 0.01&gt;p, ***: 0.001&gt;p; G1 vs G2, G3, G4. #: 0.05&gt;p, ##: 0.01&gt;p, ###: 0.001&gt;p; G2 vs G3, G4. 
         FIG.  74    shows results of Micro CT: Cortical Area Analysis. BMD: Bone Mineral Density. BV: Cortical Bone Volume. Tt/Ar: Total cross-sectional area inside the periosteal envelope. Ct.Ar: Cortical bone area. Ma.Ar: Medullary (or marrow) area. Ct.Ar/Tt.Ar: Cortical area fraction. Ct.Th: Average cortical thickness. Ps.Pm: Periosteal perimeter. Ec.Pm: Endocortical perimeter. G1; n=7, G2; n=7, G3; n=7, G4; n=7. Statistical analysis. All values were presented as mean±standard error (SEM). All values were statistically analyzed by one-way ANOVA with LSD post-hoc analysis. *: 0.05&gt;p, **: 0.01&gt;p, ***: 0.001&gt;p; G1 vs G2, G3, G4. #: 0.05&gt;p, ##: 0.01&gt;p, ###: 0.001&gt;p; G2 vs G3, G4. 
         FIG.  75    shows Micro CT: Femur 2D Images. 
         FIG.  76    shows Micro CT: Trabecular 3D Images. 
         FIG.  77    shows exemplary micrographs of inflammation and oxidative markers. 
         FIG.  78    shows an exemplary graph of TNF-α measurement. Statistical analysis: All values were presented as mean±standard error (SEM). All values were statistically analyzed by one-way ANOVA with LSD post-hoc analysis. *: 0.05&gt;p, **: 0.01&gt;p, ***: 0.001&gt;p; G1 vs All group. #: 0.05&gt;p, ##: 0.01&gt;p, ###: 0.001&gt;p; G2 vs G3, G4, G5, G6. 
         FIG.  79    shows exemplary nitrotyrosine histology stains. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Definitions 
     According to the convention used in the art, 
     
       
         
         
             
             
         
       
     
     in the formulae herein is used to indicate that a moiety or substituent “R” is attached to a backbone structure. 
     “Alkyl” is a hydrocarbon having primary, secondary, tertiary, and/or quaternary carbon atoms, and encompasses straight, branched, and cyclic groups, or a combination thereof. For example, an alkyl group may have 1 to 20 carbon atoms (i.e., C 1 -C 20  alkyl), 1 to 10 carbon atoms (i.e., C 1 -C 10  alkyl), or 1 to 6 carbon atoms (i.e., C 1 -C 6  alkyl). Examples of a suitable alkyl group include methyl (Me, —CH 3 ), ethyl (Et, —CH 2 CH 3 ), 1-propyl (n-Pr, n-propyl, —CH 2 CH 2 CH 3 ), 2-propyl (i-Pr, i-propyl, —CH(CH 3 ) 2 ), 1-butyl (n-Bu, n-butyl, —CH 2 CH 2 CH 2 CH 3 ), 2-methyl-1-propyl (i-Bu, i-butyl, —CH 2 CH(CH 3 ) 2 ), 2-butyl (s-Bu, s-butyl, —CH(CH 3 )CH 2 CH 3 ), 2-methyl-2-propyl (t-Bu, t-butyl, —C(CH 3 ) 3 ), 1-pentyl (n-pentyl, —CH 2 CH 2 CH 2 CH 2 CH 3 ), 2-pentyl (—CH(CH 3 )CH 2 CH 2 CH 3 ), 3-pentyl (—CH(CH 2 CH 3 ) 2 ), 2-methyl-2-butyl (—C(CH 3 ) 2 CH 2 CH 3 ), 3-methyl-2-butyl (—CH(CH 3 )CH(CH 3 ) 2 ), 3-methyl-1-butyl (—CH 2 CH 2 CH(CH 3 ) 2 ), 2-methyl-1-butyl (—CH 2 CH(CH 3 )CH 2 CH 3 ), 1-hexyl (—CH 2 CH 2 CH 2 CH 2 CH 2 CH 3 ), 2-hexyl (—CH(CH 3 )CH 2 CH 2 CH 2 CH 3 ), 3-hexyl (—CH(CH 2 CH 3 )(CH 2 CH 2 CH 3 )), 2-methyl-2-pentyl (—C(CH 3 ) 2 CH 2 CH 2 CH 3 ), 3-methyl-2-pentyl (—CH(CH 3 )CH(CH 3 )CH 2 CH 3 ), 4-methyl-2-pentyl (—CH(CH 3 )CH 2 CH(CH 3 ) 2 ), 3-methyl-3-pentyl (—C(CH 3 )(CH 2 CH 3 ) 2 ), 2-methyl-3-pentyl (—CH(CH 2 CH 3 )CH(CH 3 ) 2 ), 2,3-dimethyl-2-butyl (—C(CH 3 ) 2 CH(CH 3 ) 2 ), 3,3-dimethyl-2-butyl (—CH(CH 3 )C(CH 3 ) 3 ), and octyl (—(CH 2 ) 7 CH 3 ), but it is not limited thereto. 
     “Alkoxy” refers to a group having the formula —O-alkyl, wherein the alkyl group as defined above is attached to the parent compound via an oxygen atom. The alkyl moiety of the alkoxy group may have, for example, 1 to 20 carbon atoms (i.e., C 1 -C 20  alkoxy), 1 to 12 carbon atoms (i.e., C 1 -C 12  alkoxy), 1 to 10 carbon atoms (i.e., C 1 -C 10  alkoxy), or 1 to 6 carbon atoms (i.e., C 1 -C 6  alkoxy). Examples of a suitable alkoxy group include methoxy (—O—CH 3  or —OMe), ethoxy (—OCH 2 CH 3  or —OEt), and t-butoxy (—OC(CH 3 ) 3  or —O-tBu), but it is not limited thereto. 
     “Haloalkyl” is an alkyl group in which at least one of the hydrogen atoms of the alkyl group as defined above is replaced by a halogen atom. The alkyl moiety of the haloalkyl group may have 1 to 20 carbon atoms (i.e., C 1 -C 20  haloalkyl), 1 to 12 carbon atoms (i.e., C 1 -C 12  haloalkyl), 1 to 10 carbon atoms (i.e., C 1 -C 10  haloalkyl), or 1 to 6 carbon atoms (i.e., C 1 -C 6  haloalkyl). Examples of a suitable haloalkyl group include —CF 3 , —CHF 2 , —CFH 2 , and —CH 2 CF 3 , but it is not limited thereto. 
     “Alkenyl” is a hydrocarbon having primary, secondary, tertiary, and/or quaternary carbon atoms, and encompasses straight, branched, and cyclic groups, or a combination thereof, and having at least one unsaturated region, i.e., a carbon-carbon sp 2  double bond. For example, an alkenyl group may have 2 to 20 carbon atoms (i.e., C 2 -C 20  alkenyl), 2 to 12 carbon atoms (i.e., C 2 -C 12  alkenyl), 2 to 10 carbon atoms (i.e., C 2 -C 10  alkenyl), or 2 to 6 carbon atoms (i.e., C 2 -C 6  alkenyl). Examples of a suitable alkenyl group include vinyl (—CH═CH 2 ), allyl (—CH 2 CH═CH 2 ), cyclopentenyl (—C 5 H 7 ), and 5-hexenyl (—CH 2 CH 2 CH 2 CH 2 CH═CH 2 ), but it is not limited thereto. 
     “Alkynyl” is a hydrocarbon having primary, secondary, tertiary, and/or quaternary carbon atoms, and encompasses straight, branched, and cyclic groups, or a combination thereof, and having at least one carbon-carbon sp triple bond. For example, an alkynyl group may have 2 to 20 carbon atoms (i.e., C 2 -C 20  alkynyl), 2 to 12 carbon atoms (i.e., C 2 -C 12  alkynyl), 2 to 10 carbon atoms (i.e., C 2 -C 10  alkynyl), or 2 to 6 carbon atoms (i.e., C 2 -C 6  alkynyl). Examples of a suitable alkenyl group include acetylenic (—C≡CH) and propargyl (—CH 2 C≡CH), but it is not limited thereto. 
     “Alkylene” refers to a saturated hydrocarbon group that may be branched, straight, or cyclic (or may have a combination of branched, straight, or cyclic moeities) and has two valencies derived by a removal of two hydrogen atoms from the same carbon atom or two different carbon atoms of a parent alkane. For example, an alkylene group may have 1 to 20 carbon atoms, 1 to 10 carbon atoms, or 1 to 6 carbon atoms. Examples of a typical alkylene group include 1,2-ethylene (—CH 2 —CH 2 —), but it is not limited thereto. 
     “Alkenylene” refers to an unsaturated hydrocarbon group that may be branched, straight, or cyclic (or may have a combination of branched, straight, or cyclic moeities) and has two valencies derived by a removal of two hydrogen atoms from the same carbon atom or two different carbon atoms of a parent alkene. For example, an alkenylene group may have 1 to 20 carbon atoms, 1 to 10 carbon atoms, or 1 to 6 carbon atoms. Examples of a typical alkenylene group include 1,2-ethenylene (—CH═CH—), but it is not limited thereto. 
     “Alkynylene” refers to an unsaturated hydrocarbon group that may be branched, straight, or cyclic (or may have a combination of branched, straight, or cyclic moeities) and has two valencies derived by a removal of two hydrogen atoms from the same carbon atom or two different carbon atoms of a parent alkyne. For example, an alkynylene group may have 1 to 20 carbon atoms, 1 to 10 carbon atoms, or 1 to carbon atoms. Examples of a typical alkynylene radical include acetylenylene (—C≡C), propargylene (—CH 2 C≡C—), and 4-pentynylene (—CH 2 CH 2 CH 2 C≡C—), but it is not limited thereto. 
     “Aryl” refers to an aromatic hydrocarbon group. For example, an aryl group may have 6 to 20 carbon atoms, 6 to 14 carbon atoms, or 6 to 12 carbon atoms. Examples of a typical aryl group include a radical derived from benzene (e.g., phenyl), substituted benzene, substituted or unsubstituted naphthalene, substituted or unsubstituted anthracene, and the like, but it is not limited thereto. 
     “Arylalkyl” refers to an acyclic alkyl group in which one hydrogen atom bonded to a carbon atom, typically a terminal or other sp 3  carbon atom, is replaced by an aryl group. Examples of a typical arylalkyl group include benzyl, 2-phenylethan-1-yl, naphthylmethyl, 2-naphthylethan-1-yl, naphthobenzyl, 2-naphthophenylethan-1-yl, and the like (each of which may be substituted or unsubstituted), but it is not limited thereto. An arylalkyl group may have 7 to 20 carbon atoms. For example, the alkyl moiety thereof may have 1 to 6 carbon atoms, and the aryl moiety thereof may have 6 to 14 carbon atoms. 
     “Arylalkenyl” refers to an acyclic alkenyl group in which one of the hydrogen atoms bonded to a carbon atom, typically a terminal or other sp 3  carbon atom, although an sp 2  carbon atom may also be used, is replaced by an aryl group. The aryl moiety of the arylalkenyl may be, for example, any aryl group described herein, and the alkenyl moiety of the arylalkenyl may comprise, for example, any of the alkenyl groups described herein. An arylalkenyl group may have 8 to 20 carbon atoms. For example, the alkenyl moiety thereof may have 2 to 6 carbon atoms, and the aryl moiety thereof may have 6 to 14 carbon atoms. 
     “Arylalkynyl” refers to an acyclic alkynyl group in which one of the hydrogen atoms bonded to a carbon atom, typically a terminal or other sp 3  carbon atom, although an sp carbon atom may also be used, is replaced by an aryl group. The aryl moiety of the arylalkynyl may be, for example, any aryl group described herein, and the alkynyl moiety of the arylalkynyl may comprise, for example, any of the alkynyl groups described herein. An arylalkynyl group may have 8 to 20 carbon atoms. For example, the alkynyl moiety thereof may have 2 to 6 carbon atoms, and the aryl moiety thereof may have 6 to 14 carbon atoms. 
     “Cycloalkyl” refers to a saturated monocycle or polycycle that comprises only carbon atoms in the ring. A cycloalkyl group may have 3 to 7 carbon atoms as a monocycle, 7 to 12 carbon atoms as a bicycle, and up to about 20 carbon atoms as a polycycle. A monocyclic cycloalkyl has 3 to 7 ring atoms, more typically 5 or 6 ring atoms. A bicyclic cycloalkyl may have 7 to 12 ring atoms and may be a fused ring system, a spirocyclic ring system, or a bridged ring system. In exemplary cycloalkyl groups, the atoms may be arranged in a bicyclo[4,5], [5,5], [5,6], or [6,6] system. Non-limiting examples of a monocyclic cycloalkyl include cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl (each of which may be substituted or unsubstituted). 
     The term “substituted” with respect to alkyl, alkylene, aryl, arylalkyl, heterocyclyl, and the like, for example, “substituted alkyl,” “substituted alkylene,” “substituted aryl,” “substituted arylalkyl,” “substituted heterocyclyl,” and “substituted carbocyclyl (e.g., substituted cycloalkyl),” means that at least one hydrogen atom of the alkyl, alkylene, aryl, arylalkyl, heterocyclyl, or carbocyclyl (e.g., cycloalkyl) is each independently replaced by a non-hydrogen substituent. Examples of the typical substituent include halo, haloalkyl, oxo, —CN, —NO 2 , ═N—OH, —N 3 , —R, —OR, —SR, —N(R) 2 , —N(R) 3   + , ═NR, —NHC(═O)R, —C(═O)R, —C(═O)N(R) 2 , —S(═O) 2 R, —OS(═O) 2 OR, —S(═O) 2 OR, —S(═O) 2 N(R) 2 , —S(═O)R, —OP(═O)(OR) 2 , -(alkylene)-C(═O)R, —C(═S)R, —C(═O)OR, -(alkylene)-C(═O)OR, —C(═S)OR, —C(═O)SR, —C(═S)SR, -(alkylene)-C(═O)N(R) 2 , —C(═S)N(R) 2 , and —C(—NR)N(R) 2 , and R is independently H, alkyl, aryl, arylalkyl, or heterocyclyl, but it is not limited thereto. The alkylene, alkenylene, and alkynylene groups may also be similarly substituted. 
     Those skilled in the art will understand that when a moiety such as “alkyl,” “aryl,” “heterocyclyl,” and the like is substituted with at least one substituent, they may optionally be referred to as a moiety of “alkylene,” “arylene,” “heterocyclylene,” or the like (that is, at least one hydrogen atom of the parent “alkyl,” “aryl,” or “heterocyclyl” moiety is replaced by the substituent as described herein). If the moiety of “alkyl,” “aryl,” “heterocyclyl,” or the like is described herein as “substituted” or depicted in the drawings as substituted (or optionally substituted, for example, the number of substituents is 0 or a positive number), the term “alkyl,” “aryl,” “heterocyclyl,” or the like should be understood to be interchangeable with “alkylene,” “arylene,” “heterocyclylene,” or the like. 
     Those skilled in the art will recognize that the substituents and other moieties of the compound of Formula I should be selected so as to provide a compound that is sufficiently stable as a pharmaceutically useful compound that can be formulated into an acceptably stable pharmaceutical composition. The compound of Formula I having such stability is to be understood to fall within the scope of the present invention. 
     “Heteroalkyl” refers to an alkyl group in which at least one carbon atom is replaced by a heteroatom such as O, N, or S. For example, if a carbon atom of the alkyl group attached to a parent molecule is replaced by a heteroatom (e.g., O, N, or S), the resulting heteroalkyl group may be an alkoxy group (e.g., —OCH 3 ), an amine group (e.g., —NHCH 3 , —N(CH 3 ) 2 , or the like), or a thioalkyl group (e.g., —SCH 3 ), respectively. If a non-terminal carbon atom of the alkyl group that is not attached to a parent molecule is replaced by a heteroatom (e.g., O, N, or S), the resulting heteroalkyl group may be an alkyl ether (e.g., —CH 2 CH 2 —O—CH 3  or the like), an alkylamine (e.g., —CH 2 NHCH 3 , —CH 2 N(CH 3 ) 2 , or the like), or a thioalkyl ether (e.g., —CH 2 —S—CH 3 ), respectively. If the terminal carbon atom of the alkyl group is replaced by a heteroatom (for example, O, N, or S), the resulting heteroalkyl group may be a hydroxyalkyl group (e.g., —CH 2 CH 2 —OH), an aminoalkyl group (e.g., —CH 2 NH 2 ), or an alkylthiol group (e.g., —CH 2 CH 2 —SH), respectively. For example, a heteroalkyl group may have 1 to 20 carbon atoms, 1 to 10 carbon atoms, or 1 to 6 carbon atoms. Preferably, a heteroalkyl group has from 2 to 20, 2 to 10, or 2 to 6 total atoms in the chain (i.e., carbon atoms plus heteroatoms combined). A C 1 -C 6  heteroalkyl group refers to a heteroalkyl group having 1 to 6 carbon atoms. 
     The term “heterocycle” or “heterocyclyl” used herein includes those described in the documents such as Paquette, Leo A., Principles of Modern Heterocyclic Chemistry (W. A. Benjamin, New York, 1968), specifically Chapters 1, 3, 4, 6, 7, and 9; The Chemistry of Heterocyclic Compounds, A Series of Monographs (John Wiley &amp; Sons, New York, from 1950 to the present), specifically Volumes 13, 14, 16, 19, and 28; and J. Am. Chem. Soc. (1960) 82:5566, but it is not limited thereto. In a specific embodiment of the present invention, “heterocycle” includes carbocycle as defined herein in which at least one (e.g., 1, 2, 3, or 4) carbon atom is replaced by a heteroatom (e.g., O, N, or S). The term “heterocycle” or “heterocyclyl” includes saturated, partially unsaturated, and aromatic rings (i.e., a heteroaromatic ring). Substituted heterocycle, for example, includes a heterocyclic ring substituted with any of the substituents disclosed herein, inclusive of a carbonyl group. 
     Exemplary heterocycles include pyridyl, dihydropyridyl, tetrahydropyridyl(piperidyl), thiazolyl, tetrahydrothiophenyl, sulfur-oxidized tetrahydrothiophenyl, pyrimidinyl, furanyl, thienyl, pyrrolyl, pyrazolyl, imidazolyl, tetrazolyl, benzofuranyl, thianaphthenyl, indolyl, indolenyl, quinolinyl, isoquinolinyl, benzimidazolyl, piperidinyl, 4-piperidinyl, pyrrolidinyl, 2-pyrrolidonyl, pyrrolinyl, tetrahydrofuranyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, octahydroisoquinolinyl, azocynyl, triazinyl, 6H-1,2,5-thiadiazinyl, 2H,6H-1,5,2-dithiazinyl, thianthrenyl, pyranyl, isobenzofuranyl, chromenyl, xanthenyl, phenoxatinyl, 2H-pyrrolyl, isothiazolyl, isoxazolyl, pyrazinyl, pyridazinyl, indolizinyl, isoindolyl, 3H-indolyl, 1H-indazoly, purinyl, 4H-quinolizinyl, phthalazinyl, naphthyridinyl, quinoxalinyl, quinazolinyl, cinnolinyl, phtheridinyl, 4aH-carbazolyl, carbazolyl, β-carbolinyl, phenanthridinyl, acridinyl, pyrimidinyl, phenanthrolinyl, phenazinyl, phenothiazinyl, furazanyl, phenoxazinyl, isochromanyl, chromanyl, imidazolidinyl, imidazolinyl, pyrazolidinyl, pyrazolinyl, piperazinyl, indolinyl, isoindolinyl, quinuclidinyl, morpholinyl, oxazolidinyl, benzotriazolyl, benzisoxazolyl, oxindolyl, and benzoxazolinyl (each of which may be substituted or unsubstituted), but it is not limited thereto. 
     As an example, a carbon-bonded heterocycle may be bonded at the 2, 3, 4, 5, or 6-position of pyrazine, at the 3, 4, 5, or 6-position of pyridazine, at the 2, 4, 5, or 6-position of pyrimidine, at the 2, 3, 5, or 6-position of pyrazine, at the 2, 3, 4, or 5-position of furan, tetrahydrofuran, thiofuran, thiophene, pyrrole, or tetrahydropyrrole, at the 2, 4, or 5-position of oxazole, imidazole, or thiazole, at the 3, 4, or 5-position of isoxazole, pyrazole, or isothiazole, at the 2 or 3-position of aziridine, at the 2, 3, or 4-position of azetidine, at the 2, 3, 4, 5, 6, 7, or 8-position of quinoline, or at the 1, 3, 4, 5, 6, 7, or 8-position of isoquinoline, but it is not limited thereto. More typically, examples of a carbon-bonded heterocycle include 2-pyridyl, 3-pyridyl, 4-pyridyl, 5-pyridyl, 6-pyridyl, 3-pyridazinyl, 4-pyridazinyl, 5-pyridazinyl, 6-pyridazinyl, 2-pyrimidinyl, 4-pyrimidinyl, 5-pyrimidinyl, 6-pyrimidinyl, 2-pyrazinyl, 3-pyrazinyl, 5-pyrazinyl, 6-pyrazinyl, 2-thiazolyl, 4-thiazolyl, and 5-thiazolyl (each of which may be substituted or unsubstituted). 
     As an example, a nitrogen-bonded heterocycle may be bonded at the 1-position of aziridine, azetidine, pyrrole, pyrrolidine, 2-pyrroline, 3-pyrroline, imidazole, imidazolidine, 2-imidazoline, 3-imidazoline, pyrazole, pyrazoline, 2-pyrazoline, 3-pyrazoline, piperidine, piperazine, indole, indoline, or 1H-indazole, at the 2-position of isoindole or isoindoline, at the 4-position of morpholine, and at the 9-position of carbazole or β-carboline (each of which may be substituted or unsubstituted), but it is not limited thereto. More typically, examples of a nitrogen-bonded heterocycle include 1-aziridinyl, 1-azetidyl, 1-pyrrolyl, 1-imidazolyl, 1-pyrazolyl, and 1-piperidinyl (each of which may be substituted or unsubstituted). 
     “Heterocyclylalkyl” refers to an acyclic alkyl radical in which one hydrogen atom bonded to a carbon atom, typically a terminal or sp 3  carbon atom, is replaced by a heterocyclyl radical (i.e., a heterocyclyl-alkylene moiety). Examples of a typical heterocyclylalkyl group include heterocyclyl-CH 2 —, 2-(heterocyclyl)ethan-1-yl, and the like, but it is not limited thereto. The “heterocyclyl” moiety thereof used herein includes those described in the document such as “Principles of Modern Heterocyclic Chemistry” and any heterocyclyl group described above. Those skilled in the art will understand that if the resulting group is chemically stable, the heterocyclyl group may be attached to the alkyl moiety of the heterocyclylalkyl through a carbon-to-carbon bond or a carbon-to-heteroatom bond. A heterocyclylalkyl group may have 2 to 20 carbon atoms. For example, the alkyl moiety of the heterocyclylalkyl group may have 1 to 6 carbon atoms, and the heterocyclyl moiety thereof may have 2 to 14 carbon atoms. Examples of the heterocyclylalkyl include a 5-membered heterocycle containing sulfur, oxygen, and/or nitrogen such as thiazolylmethyl, 2-thiazolylethan-1-yl, imidazolylmethyl, oxazolylmethyl, thiadiazolylmethyl, and the like; and a 6-membered heterocycle containing sulfur, oxygen, and/or nitrogen such as piperidinylmethyl, piperazinylmethyl, morpholinylmethyl, pyridinylmethyl, pyridazylmethyl, pyrimidylmethyl, pyrazinylmethyl, and the like (each of which may be substituted or unsubstituted), but it is not limited thereto. 
     “Heterocyclylalkenyl” refers to an acyclic alkenyl radical in which one of the hydrogen atoms bonded to a carbon atom, typically a terminal or sp 3  carbon atom although an sp 2  carbon atom may also be used, is replaced by a heterocyclyl radical (i.e., a heterocyclyl-alkenylene moiety). The heterocyclyl moiety of the heterocyclylalkenyl group includes those described in the document such as “Principles of Modern Heterocyclic Chemistry” and any heterocyclyl group described herein. The alkenyl moiety of the heterocyclylalkenyl group includes any alkenyl group described herein. Those skilled in the art will understand that if the resulting group is chemically stable, the heterocyclyl group may be attached to the alkenyl moiety of the heterocyclylalkenyl via a carbon-to-carbon bond or a carbon-to-heteroatom bond. A heterocyclylalkenyl group may have 3 to 20 carbon atoms. For example, the alkenyl moiety of the heterocyclylalkenyl group may have 2 to 6 carbon atoms, and the heterocyclyl moiety thereof may have 2 to 14 carbon atoms. 
     “Heterocyclylalkynyl” refers to an acyclic alkynyl radical in which one of the hydrogen atoms bonded to a carbon atom, typically a terminal or sp 3  carbon atom although an sp carbon atom may also be used, is replaced by a heterocyclyl radical (i.e., a heterocyclyl-alkynylene moiety). The heterocyclyl moiety of the heterocyclylalkynyl group includes those described in the document such as “Principles of Modern Heterocyclic Chemistry” and any heterocyclyl group described herein. The alkynyl moiety of the heterocyclylalkynyl group includes any alkynyl group described herein. Those skilled in the art will understand that if the resulting group is chemically stable, the heterocyclyl group may be attached to the alkynyl moiety of the heterocyclylalkynyl via a carbon-to-carbon bond or a carbon-to-heteroatom bond. A heterocyclylalkynyl group may have 3 to 20 carbon atoms. For example, the alkynyl moiety of the heterocyclylalkynyl group may have 2 to 6 carbon atoms, and the heterocyclyl moiety thereof may have 2 to 14 carbon atoms. 
     “Heteroaryl” refers to an aromatic heterocyclyl containing at least one heteroatom in the ring. Non-limiting examples of a suitable heteroatom that may be contained in the aromatic ring include oxygen, sulfur, and nitrogen. Non-limiting examples of a heteroaryl ring include all of those enumerated in the definition of “heterocyclyl” herein, inclusive of pyridinyl, pyrrolyl, oxazolyl, indolyl, isoindolyl, furanyl, thienyl, benzofuranyl, benzothiophenyl, carbazolyl, imidazolyl, thiazolyl, isoxazolyl, pyrazolyl, isothiazolyl, quinolyl, isoquinolyl, pyridazyl, pyrimidyl, pyrazyl, and the like (each of which may be substituted or unsubstituted). 
     “Carbocycle” or “carbocyclyl” refers to a saturated, partially unsaturated, or aromatic ring having 3 to 7 carbon atoms as a monocycle, 7 to 12 carbon atoms as a bicycle, and up to about 20 carbon atoms as a polycycle. A monocyclic carbocycle has 3 to 7 ring atoms, more typically 5 or 6 ring atoms. A bicyclic cycloalkyl may have 7 to 12 ring atoms and may be a fused ring system, a spirocyclic ring system, or a bridged ring system. In exemplary cycloalkyl groups, the atoms are arranged in a bicyclo[4,5], [5,5], [5,6], or [6,6] system. Non-limiting examples of a monocyclic cycloalkyl include cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl (each of which may be substituted or unsubstituted). 
     “Acyl” refers to —C(═O)-alkyl, —C(═O)-carbocycle (which is substituted or unsubstituted), and —C(═O)-heterocycle (which is substituted or unsubstituted), wherein the alkyl, carbocycle, or heterocycle moiety is as defined herein. Non-limiting examples of “acyl” include —C(═O)CH 3 , —C(═O)CH 2 CH 3 , —C(═O)CH(CH 3 ) 2 , —C(═O)C(CH 3 ) 3 , —C(═O)-phenyl (which is substituted or unsubstituted), —C(═O)-cyclopropyl (which is substituted or unsubstituted), —C(═O)-cyclobutyl (which is substituted or unsubstituted), —C(═O)-cyclopentyl (which is substituted or unsubstituted), —C(═O)-cyclohexyl (which is substituted or unsubstituted), and —C(═O)-pyridyl (which is substituted or unsubstituted). 
     “Arylheteroalkyl” refers to a heteroalkyl as defined herein, wherein a hydrogen atom (which may be attached to either a carbon atom or a heteroatom) is replaced by an aryl group as defined herein. If the resulting group is chemically stable, the aryl group may be attached to a carbon atom of the heteroalkyl group or the heteroatom of the heteroalkyl group. For example, an arylheteroalkyl group may have a formula of -alkylene-O-aryl, -alkylene-O-alkylene-aryl, -alkylene-NH-aryl, -alkylene-NH-alkylene-aryl, -alkylene-S-aryl, -alkylene-S-alkylene-aryl, or the like. In addition, any alkylene moiety in the above formulae may be further substituted with any of the substituents defined or exemplified herein. 
     “Heteroarylalkyl” refers to an alkyl group as defined herein, wherein a hydrogen atom is replaced by a heteroaryl group as defined herein. Non-limiting examples of heteroarylalkyl include —CH 2 -pyridinyl, —CH 2 -pyrrolyl, —CH 2 -oxazolyl, —CH 2 -indolyl, —CH 2 -isoindolyl, —CH 2 -furanyl, —CH 2 -thienyl, —CH 2 -benzofuranyl, —CH 2 -benzothiophenyl, —CH 2 -carbazolyl, —CH 2 -imidazolyl, —CH 2 -thiazolyl, —CH 2 -isoxazolyl, —CH 2 -pyrazolyl, —CH 2 -isothiazolyl, —CH 2 -quinolyl, —CH 2 -isoquinolyl, —CH 2 -pyridazyl, —CH 2 -pyrimidyl, —CH 2 -pyrazyl, —CH(CH 3 )-pyridinyl, —CH(CH 3 )-pyrrolyl, —CH(CH 3 )-oxazolyl, —CH(CH 3 )-indolyl, —CH(CH 3 )-isoindolyl, —CH(CH 3 )-furanyl, —CH(CH 3 )-thienyl, —CH(CH 3 )-benzofuranyl, —CH(CH 3 )-benzothiophenyl, —CH(CH 3 )-carbazolyl, —CH(CH 3 )-imidazolyl, —CH(CH 3 )-thiazolyl, —CH(CH 3 )-isoxazolyl, —CH(CH 3 )-pyrazolyl, —CH(CH 3 )-isothiazolyl, —CH(CH 3 )-quinolyl, —CH(CH 3 )-isoquinolyl, —CH(CH 3 )-pyridazyl, —CH(CH 3 )-pyrimidyl, —CH(CH 3 )-pyrazyl, and the like. 
     “Silyloxy” refers to the group —O—SiR 3 , wherein each R independently is alkyl, aryl (which is substituted or unsubstituted), or heteroaryl (which is substituted or unsubstituted). Non-limiting examples of silyloxy include —O—Si(CH 3 ) 3 , —O—Si(CH 3 ) 2 tBu, —O—Si(tBu) 2 CH 3 , —O—Si(tBu) 3 , —O—Si(CH 3 ) 2 Ph, —O—Si(Ph) 2 CH 3 , and —O—Si(Ph) 3 . 
     The term “optionally substituted” refers to a particular moiety (e.g., an optionally substituted aryl group) of the compound of Formula I that optionally has one, two, or more substituents. 
     The term “ester thereof” refers to any ester of a compound wherein any —COOH functional group of the molecule is modified to be a —COOR functional group or any —OH functional group of the molecule is modified to be a —OC(═O)R. Here, the R moiety of the ester may be any carbon-containing group that forms a stable ester moiety, which includes, but is not limited to, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heterocyclyl, heterocyclylalkyl, and substituted derivatives thereof. Examples of the ester may also include an ester such as those described above of a “tautomeric enol” as described below. 
     Compounds 
     In certain embodiments, the invention provides a compound represented by Formula (I): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof
 
wherein:
 
     R 1 , R 2 , and R 3  are each independently H or substituted or unsubstituted alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl; 
     R 4 , independently for each occurrence, is selected from substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, oxo, —OR b , —CH 2 OR b , halo, hydroxyl, and hydroxyalkyl;
         R b  is substituted or unsubstituted alkyl, aryl, arylalkyl, or heterocyclyl;       

     p is 0, 1, or 2; 
     R 6  is hydrogen or substituted or unsubstituted alkyl; and 
     R 7 , R 8 , and R 9  are each independently hydrogen or alkyl. 
     preferably wherein the compound comprises at least one D-amino acid residue. 
     In certain embodiments, the invention provides a compound represented by Formula (I), or a pharmaceutically acceptable salt thereof, wherein the compound is not: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 1 , R 2 , and R 3  are each independently H or substituted or unsubstituted alkyl, arylalkyl, or heterocyclylalkyl; 
     R 4 , independently for each occurrence, is selected from substituted or unsubstituted alkyl, oxo, hydroxyl, —OR b , hydroxyalkyl, —CH 2 OR b , and halo;
         R b  is substituted or unsubstituted alkyl, aryl, arylalkyl, or heterocyclyl;       

     R 6  is hydrogen or substituted or unsubstituted alkyl; and 
     R 7 , R 8 , and R 9  are each independently hydrogen or alkyl. 
     In some embodiments, where indicated, alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl is unsubstituted or is substituted with one or more substituents selected from halo, haloalkyl, oxo, —CN, —NO 2 , ═N—OH, —N 3 , —R a , —OR a , —SR a , —N(R a ) 2 , —N(R a ) 3   + , —NR a , —NHC(═O)R c , —C(═O)R c , —C(═O)N(R a ) 2 , —S(═O) 2 R c , —OS(═O) 2 OR a , —S(═O) 2 OR a , —S(═O) 2 N(R a ) 2 , —S(═O)R c , —OP(═O)(OR a ) 2 , -(alkylene)-C(═O)R c , —C(═S)R c , —C(═O)OR a , -(alkylene)-C(═O)OR a , —C(═S)OR a , —C(═O)SR a , —C(═S)SR a , -(alkylene)-C(═O)N(R a ) 2 , —C(═S)N(R a ) 2 , and —C(—NR a )N(R a ) 2 ; 
     R a , independently for each occurrence, is hydrogen, or substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, heterocyclylalkyl, cycloalkyl, or (cycloalkyl)alkyl; and 
     R c , independently for each occurrence, is substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, heterocyclylalkyl, cycloalkyl, or (cycloalkyl)alkyl. 
     In more particular embodiments, where indicated, alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl is unsubstituted or is substituted with one or more substituents selected from halo, haloalkyl, oxo, —R a , —OR a , —N(R a ) 2 , —N(R a ) 3   + , —NHC(═O)R c , —C(═O)R c , —C(═O)N(R a ) 2 , —C(═O)OR a , -(alkylene)-C(═O)OR a , and -(alkylene)-C(═O)N(R a ) 2 ; 
     R a , independently for each occurrence, is hydrogen, or substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, heterocyclylalkyl, cycloalkyl, or (cycloalkyl)alkyl; and 
     R c , independently for each occurrence, is substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, heterocyclylalkyl, cycloalkyl, or (cycloalkyl)alkyl. 
     In certain such embodiments, R a , independently for each occurrence, is hydrogen, alkyl, aryl, arylalkyl, heterocyclyl, or heterocyclylalkyl; and R c , independently for each occurrence, is alkyl, aryl, arylalkyl, heterocyclyl, or heterocyclylalkyl. 
     In certain embodiments, the compound has the structure of formula (I-10L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-10D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 1  is substituted or unsubstituted alkyl, arylalkyl, or heterocyclylalkyl. 
     More specifically, R 1  may be selected from substituted or unsubstituted alkyl, 
     
       
         
         
             
             
         
       
     
     R a  is hydrogen or alkyl; and
 
n is an integer from 1 to 10, preferably 1-5, more preferably 1-3.
 
     Exemplary R 1  groups include 
     
       
         
         
             
             
         
       
     
     In some preferred embodiments, R 1  is 
     
       
         
         
             
             
         
       
     
     In alternative preferred embodiments, R 1  is 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-1L). 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-1D) 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 2  is H or substituted or unsubstituted alkyl, arylalkyl, or heterocyclylalkyl. 
     In some embodiments, R 2  is selected from hydrogen, substituted or unsubstituted alkyl, 
     
       
         
         
             
             
         
       
     
     R a  is hydrogen or alkyl; and
 
n is an integer from 1 to 10, preferably 1-5, more preferably 1-3.
 
     Exemplary R 2  groups include 
     
       
         
         
             
             
         
       
     
     Preferably, R 2  is hydrogen. 
     In certain embodiments, the compound has the structure of formula (I-2L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-2D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 3  is substituted or unsubstituted alkyl or arylalkyl. 
     In some embodiments, R 3  is selected from substituted or unsubstituted alkyl, 
     
       
         
         
             
             
         
       
     
     R a  is hydrogen or alkyl; and
 
n is an integer from 1 to 10, preferably 1-5, more preferably 1-3.
 
     Exemplary R 3  groups include 
     
       
         
         
             
             
         
       
     
     Preferably, R 3  is 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-3L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-3D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, p is 1 or 2; and R 4 , independently for each occurrence, is selected from substituted or unsubstituted alkyl, —OR b , —CH 2 OR b , halo, hydroxyl, and hydroxyalkyl. 
     In certain embodiments, p is 1 or 2; and R 4 , independently for each occurrence, is selected from —CH 3 , halo, hydroxyl, and hydroxyalkyl. 
     In certain preferred embodiments, R 4  is hydroxyl. In alternative preferred embodiments, R 4  is —CH 3 . 
     In any of the above embodiments, p may be 1. 
     In certain embodiments, the compound has the structure of formula (I-4Lg): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4La): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Lb): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Lc): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Lc), provided that R 4  is not hydroxyl. 
     In certain embodiments, the compound has the structure of formula (I-4Dg): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Da): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Db): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Dc): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Dc), provided that R 4  is not hydroxyl. 
     In certain embodiments, R 4  is oxo. 
     In certain embodiments, the compound has the structure of formula (I-4Ld): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Le): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Dd): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4De): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 6  is hydrogen or alkyl, wherein the alkyl is optionally substituted with one occurrence of —C(═O)NH 2 . In certain embodiments, wherein R 6  is alkyl optionally substituted with one occurrence of —C(═O)NH 2 . For example, R 6  may be —CH 3 . Alternatively, R 6  may be 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-6L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-6D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 7  is (C 1 -C 10 )alkyl, preferably 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-7L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-7D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-11L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-11D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 8  is —CH 3  or —H, preferably —H. 
     In certain embodiments, R 9  is —CH 3  or —H, preferably —H. 
     In certain embodiments, the compound comprises at least one, at least two, at least three, at least four, at least five, at least six, at least seven, or at least eight D-amino acid residues. 
     In certain embodiments, the invention provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, wherein the compound is selected from the following: 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     The present invention also provides a compound represented by Formula (I): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof; 
     wherein: 
     R 1 , R 2 , and R 3  are each independently H or substituted or unsubstituted alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl; 
     R 4 , independently for each occurrence, is selected from substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, oxo, —OR b , —CH 2 OR b , halo, hydroxyl, and hydroxyalkyl;
         R b  is substituted or unsubstituted alkyl, aryl, arylalkyl, or heterocyclyl;       

     p is 0, 1, or 2; 
     R 6  is hydrogen or substituted or unsubstituted alkyl; and 
     R 7 , R 8 , and R 9  are each independently hydrogen or alkyl; wherein at least one of: 
     (a) at least one of R 1 , R 2 , and R 3  is substituted or unsubstituted (C 2 -C 10 )haloalkyl; 
     (b) at least one of alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl is substituted with one or more substituents selected from —R a , —OR a , —SR a , —N(R a ) 2 , —N(R a ) 3   + , —NR a , —NHC(═O)R c , —C(═O)R c , —C(═O)N(R a ) 2 , —S(═O) 2 R c , —OS(═O) 2 OR a , —S(═O) 2 OR a , —S(═O) 2 N(R a ) 2 , —S(═O)R c , —OP(═O)(OR a ) 2 , -(alkylene)-C(═O)R c , —C(═S)R c , —C(═O)OR a , -(alkylene)-C(═O)OR a , —C(═S)OR a , —C(═O)SR a , —C(═S)SR a , -(alkylene)-C(═O)N(R a ) 2 , —C(═S)N(R a ) 2 , and —C(—NR a )N(R a ) 2 ; and 
     at least one occurrence of R a  or R c  is heterocyclylalkyl, cycloalkyl, or (cycloalkyl)alkyl; 
     (c) the compound comprises at least one D-amino acid residue; or 
     (d) at least two occurrences of R a ;
         at least two occurrences of R c ; or   at least one occurrence of R a  and at least one occurrence of R c ; and   at least one occurrence of R a  and/or R c  differs from the other occurrences.       

     In certain embodiments, at least one of R 1 , R 2 , and R 3  is substituted or unsubstituted (C 2 -C 10 )haloalkyl. 
     In certain embodiments, at least one of alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl is substituted with one or more substituents selected from —R a , OR a , —SR a , —N(R a ) 2 , —N(R a ) 3   + , ═NR a , —NHC(═O)R c , —C(═O)R c , —C(═O)N(R a ) 2 , —S(═O) 2 R c , —OS(═O) 2 OR a , —S(═O) 2 OR a , —S(═O) 2 N(R a ) 2 , —S(═O)R c , —OP(═O)(OR a ) 2 , -(alkylene)-C(═O)R c , —C(═S)R c , —C(═O)OR a , -(alkylene)-C(═O)OR a , —C(═S)OR a , —C(═O)SR a , —C(═S)SR a , -(alkylene)-C(═O)N(R a ) 2 , —C(═S)N(R a ) 2 , and —C(—NR a )N(R a ) 2 ; and at least one occurrence of R a  or R c  is heterocyclylalkyl, cycloalkyl, or (cycloalkyl)alkyl. 
     In certain embodiments, the compound comprises at least one D-amino acid residue. 
     In certain embodiments, the compound has: 
     at least two occurrences of R a ; 
     at least two occurrences of R c ; or 
     at least one occurrence of R a  and at least one occurrence of R c ; and 
     at least one occurrence of R a  and/or R c  differs from the other occurrences. 
     In certain embodiments: 
     R 1 , R 2 , and R 3  are each independently H or substituted or unsubstituted alkyl, arylalkyl, or heterocyclylalkyl; 
     R 4 , independently for each occurrence, is selected from substituted or unsubstituted alkyl, oxo, hydroxyl, —OR b , hydroxyalkyl, —CH 2 OR b , and halo;
         R b  is substituted or unsubstituted alkyl, aryl, arylalkyl, or heterocyclyl;       

     R 6  is hydrogen or substituted or unsubstituted alkyl; and 
     R 7 , R 8 , and R 9  are each independently hydrogen or alkyl. 
     In some embodiments, where indicated, alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl is unsubstituted or is substituted with one or more substituents selected from halo, haloalkyl, oxo, —CN, —NO 2 , ═N—OH, —N 3 , —R a , —OR a , —SR a , —N(R a ) 2 , —N(R a ) 3   + , —NR a , —NHC(═O)R c , —C(═O)R c , —C(═O)N(R a ) 2 , —S(═O) 2 R c , —OS(═O) 2 OR a , —S(═O) 2 OR a , —S(═O) 2 N(R a ) 2 , —S(═O)R c , —OP(═O)(OR a ) 2 , -(alkylene)-C(═O)R c , —C(═S)R c , —C(═O)OR a , -(alkylene)-C(═O)OR a , —C(═S)OR a , —C(═O)SR a , —C(═S)SR a , -(alkylene)-C(═O)N(R a ) 2 , —C(═S)N(R a ) 2 , and —C(—NR a )N(R a ) 2 ; 
     R a , independently for each occurrence, is hydrogen, or substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, heterocyclylalkyl, cycloalkyl, or (cycloalkyl)alkyl; and 
     R c , independently for each occurrence, is substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, heterocyclylalkyl, cycloalkyl, or (cycloalkyl)alkyl. 
     In more particular embodiments, where indicated, alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl is unsubstituted or is substituted with one or more substituents selected from halo, haloalkyl, oxo, —R a , —OR a , —N(R a ) 2 , —N(R a ) 3   + , —NHC(═O)R c , —C(═O)R c , —C(═O)N(R a ) 2 , —C(═O)OR a , -(alkylene)-C(═O)OR a , and -(alkylene)-C(═O)N(R a ) 2 ; 
     R a , independently for each occurrence, is hydrogen, or substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, heterocyclylalkyl, cycloalkyl, or (cycloalkyl)alkyl; and 
     R c , independently for each occurrence, is substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, heterocyclylalkyl, cycloalkyl, or (cycloalkyl)alkyl. 
     In certain such embodiments, R a , independently for each occurrence, is hydrogen, alkyl, aryl, arylalkyl, heterocyclyl, or heterocyclylalkyl; and R c , independently for each occurrence, is alkyl, aryl, arylalkyl, heterocyclyl, or heterocyclylalkyl. 
     In certain embodiments, the compound has the structure of formula (I-10L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-10D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 1  is substituted or unsubstituted (C 2 -C 10 )haloalkyl. 
     In certain embodiments, R 1  is substituted or unsubstituted alkyl, arylalkyl, or heterocyclylalkyl. 
     More specifically, R 1  may be selected from substituted or unsubstituted alkyl, 
     
       
         
         
             
             
         
       
     
     R a  is hydrogen or alkyl; and
 
n is an integer from 1 to 10, preferably 1-5, more preferably 1-3.
 
     Exemplary R 1  groups include 
     
       
         
         
             
             
         
       
     
     In some preferred embodiments, R 1  is 
     
       
         
         
             
             
         
       
     
     In alternative preferred embodiments, R 1  is 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-1L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-1D) 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 2  is substituted or unsubstituted (C 2 -C 10 )haloalkyl. 
     In certain embodiments, R 2  is H or substituted or unsubstituted alkyl, arylalkyl, or heterocyclylalkyl. 
     In some embodiments, R 2  is selected from hydrogen, substituted or unsubstituted alkyl, 
     
       
         
         
             
             
         
       
     
     R a  is hydrogen or alkyl; and 
     n is an integer from 1 to 10, preferably 1-5, more preferably 1-3. 
     Exemplary R 2  groups include 
     
       
         
         
             
             
         
       
     
     Preferably, R 2  is hydrogen. 
     In certain embodiments, the compound has the structure of formula I-2L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-2D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 3  is substituted or unsubstituted (C 2 -C 10 )haloalkyl. 
     In certain embodiments, R 3  is substituted or unsubstituted alkyl or arylalkyl. 
     In some embodiments, R 3  is selected from substituted or unsubstituted alkyl, 
     
       
         
         
             
             
         
       
     
     R a  is hydrogen or alkyl; and
 
n is an integer from 1 to 10, preferably 1-5, more preferably 1-3.
 
     Exemplary R 3  groups include 
     
       
         
         
             
             
         
       
     
     Preferably, R 3  is 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-3L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-3D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, p is 1 or 2; and R 4 , independently for each occurrence, is selected from substituted or unsubstituted alkyl, —OR b , —CH 2 OR b , halo, hydroxyl, and hydroxyalkyl. 
     In certain embodiments, p is 1 or 2; and R 4 , independently for each occurrence, is selected from —CH 3 , halo, hydroxyl, and hydroxyalkyl. 
     In certain preferred embodiments, R 4  is hydroxyl. In alternative preferred embodiments, R 4  is —CH 3 . 
     In any of the above embodiments, p may be 1. 
     In certain embodiments, the compound has the structure of formula (I-4Lg): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4La): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Lb): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Lc): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Lc), provided that R 4  is not hydroxyl. 
     In certain embodiments, the compound has the structure of formula (I-4Dg): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Da): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Db): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Dc): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Dc), provided that R 4  is not hydroxyl. 
     In certain embodiments, R 4  is oxo. 
     In certain embodiments, the compound has the structure of formula (I-4Ld): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Le): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4Dd): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-4De): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 6  is hydrogen or alkyl, wherein the alkyl is optionally substituted with one occurrence of —C(═O)NH 2 . In certain embodiments, wherein R 6  is alkyl optionally substituted with one occurrence of —C(═O)NH 2 . For example, R 6  may be —CH 3 . Alternatively, R 6  may be 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-6L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-6D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 7  is (C 1 -C 10 )alkyl, preferably 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-7L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-7D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (I-11L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (I-11D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 8  is —CH 3  or —H, preferably —H. 
     In certain embodiments, R 9  is —CH 3  or —H, preferably —H. 
     In certain embodiments, the compound is 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof. 
     In certain embodiments, the compound is a peptide having an amino acid sequence represented by HyP-Gly-Gln-Xaa-Gly-Leu-Ala-Gly-Pro-Lys; 
     or a pharmaceutically acceptable salt and/or stereoisomer thereof;
     wherein Xaa is selected from Glu, Asn, Gln, His, Lys, Ser, Thr, Ala, Val, Ile, Leu, Phe, Tyr, Trp, homo-Ser, Asp(Me), and Asn(Me).   

     In certain such embodiments, at least one, at least two, at least three, at least four, at least five, at least six, or at least seven amino acid residues in the peptide are D-amino acid residues. 
     The peptide may be a variant of a collagen type II α1-derived peptide. The collagen type II α1 may be isolated from the extracellular matrix derived from animal chondrocytes. 
     The term “peptide” used in the present invention refers to a compound in which two or more amino acids are linked by a peptide bond. Further, it is classified into dipeptide, tripeptide, tetrapeptide, and the like according to the number of constituent amino acids. An oligopeptide has about 10 or fewer peptide bonds, and a polypeptide has a plurality of peptide bonds. In addition, a peptide in the present invention includes a mutated peptide in which its amino acid residue is substituted. 
     The term “HyP” used in the present invention refers to an amino acid called hydroxyproline, in which a hydroxyl group (—OH) is bonded to the carbon atom at the 4-position of proline. HyP has a structure of C 5 H 9 NO 3  and may be depicted as follows: 
     
       
         
         
             
             
         
       
     
     HyP may include all isomers. In addition, HyP may be an isomer represented by the stereochemistry of “2S,4R” unless otherwise specified. 
     The term “homo-Ser” used in the present invention is called homoserine and refers to an α-amino acid having a hydroxyl group in the side chain. Homo-Ser is an intermediate present in the biosynthesis of threonine and methionine in microorganisms and plants. Homo-Ser may be depicted as follows: 
     
       
         
         
             
             
         
       
     
     The term “Asp(Me)” used in the present invention indicates an amino acid in which the hydrogen atom of the hydroxyl group (OH) bonded to the carbon atom at the 4-position of aspartic acid is substituted by a methyl group (CH 3 ). Asp(Me) may be depicted as follows: 
     
       
         
         
             
             
         
       
     
     The term “Asn(Me)” used in the present invention indicates an amino acid in which the hydrogen atom of the amine group (NH 2 ) bonded to the carbon atom at the 4-position of asparagine is substituted by a methyl group (CH 3 ). Asn(Me) may be depicted as follows: 
     
       
         
         
             
             
         
       
     
     The term “(N-Me)Gly” used in the present invention indicates an amino acid in which the hydrogen atom of the amine group (NH 2 ) bonded to the carbon atom at the 2-position of glycine is replaced by a methyl group (CH 3 ). (N-Me)Gly may be depicted as follows: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound is a peptide having an amino acid sequence represented by HyP-Gly-Gln-Asp-Xaa-Leu-Ala-Gly-Pro-Lys; 
     or a pharmaceutically acceptable salt and/or stereoisomer thereof;
     wherein Xaa is selected from Val, Ile, Leu, Ala, Phe, Tyr, Trp, Ser, Thr, and (N-Me)Gly.   

     In certain such embodiments, at least one, at least two, at least three, at least four, at least five, at least six, or at least seven amino acid residues in the peptide are D-amino acid residues. 
     In certain embodiments, the compound is a peptide having an amino acid sequence represented by HyP-Gly-Gln-Leu-Gly-Leu-Ala-Gly-Pro-Xaa; 
     or a pharmaceutically acceptable salt and/or stereoisomer thereof;
     wherein Xaa is selected from Tyr, Leu, Glu, Gln, Ala, and Nle(6-OH).   

     In certain such embodiments, at least one, at least two, at least three, at least four, at least five, at least six, or at least seven amino acid residues in the peptide are D-amino acid residues. 
     In certain embodiments, the compound is a peptide having an amino acid sequence represented by Xaa-Gly-Gln-Leu-Gly-Leu-Ala-Gly-Pro-Lys; 
     or a pharmaceutically acceptable salt and/or stereoisomer thereof;
     wherein Xaa is selected from:   

     
       
         
         
             
             
         
       
     
     In certain such embodiments, at least one, at least two, at least three, at least four, at least five, at least six, or at least seven amino acid residues in the peptide are D-amino acid residues. 
     In certain embodiments, the invention provides a compound having the following structure: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof. 
     In certain embodiments, the invention provides a compound represented by Formula (V): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof; 
     wherein: 
     R 1  and R 2  are each independently H or substituted or unsubstituted alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl; 
     R 4 , independently for each occurrence, is selected from substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, oxo, —OR b , —CH 2 OR b , halo, hydroxyl, and hydroxyalkyl;
         R b  is substituted or unsubstituted alkyl, aryl, arylalkyl, or heterocyclyl;       

     p is 0, 1, or 2; 
     R 6  is hydrogen or substituted or unsubstituted alkyl; and 
     R 9  is hydrogen or alkyl. 
     In certain embodiments, R 1  and R 2  are each independently H or substituted or unsubstituted alkyl; 
     R 4  for each occurrence is hydroxyl; 
     p is 1; 
     R 6  is alkyl optionally substituted with one occurrence of —C(═O)NH 2 ; and 
     R 9  is hydrogen. 
     In certain embodiments, R 1  is substituted or unsubstituted alkyl, such as 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (V-1L) 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (V-1D) 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 2  is H. 
     In certain embodiments, p is 1 and R 4  is hydroxyl. 
     In certain embodiments, the compound has the structure of formula (V-4La): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (V-4Lb): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (V-4Da): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (V-4Db): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 6  is alkyl substituted with one occurrence of —C(═O)NH 2 , such as 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (V-6L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (V-6D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 9  is —H. 
     In certain embodiments, the compound is selected from the following: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof. 
     In certain embodiments, the invention provides a compound represented by Formula (VI): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof; 
     wherein: 
     R 1  and R 2  are each independently H or substituted or unsubstituted alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl 
     R 4 , independently for each occurrence, is selected from substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, oxo, —OR b , —CH 2 OR b , halo, hydroxyl, and hydroxyalkyl;
         R b  is substituted or unsubstituted alkyl, aryl, arylalkyl, or heterocyclyl;       

     p is 0, 1, or 2; 
     R 6  is hydrogen or substituted or unsubstituted alkyl; 
     R 7  is hydrogen or alkyl; and 
     R 9  is hydrogen or alkyl. 
     In certain embodiments, the invention provides a compound represented by Formula (VI), wherein the compound is not: 
     
       
         
         
             
             
         
       
     
     In certain embodiments: 
     R 1  and R 2  are each independently H or substituted or unsubstituted alkyl; 
     R 4  for each occurrence is hydroxyl; 
     p is 1; 
     R 6  is alkyl optionally substituted with one occurrence of —C(═O)NH 2 ; and 
     R 9  is hydrogen. 
     In certain embodiments, R 1  is substituted or unsubstituted alkyl, such as 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VI-1L) 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (VI-1D) 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 2  is H. 
     In certain embodiments, p is 1 and R 4  is hydroxyl. 
     In certain embodiments, the compound has the structure of formula (VI-4La): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VI-4Lb): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VI-4Da): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VI-4Db): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 6  is alkyl substituted with one occurrence of —C(═O)NH 2 , such as 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VI-6L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (VI-6D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 9  is —H. 
     In certain embodiments, R 7  is (C 1 -C 10 )alkyl, such as 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VI-7L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (VI-7D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the invention provides a compound represented by Formula (VII): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof; 
     wherein: 
     R 1  and R 2  are each independently H or substituted or unsubstituted alkyl, alkoxy, haloalkyl, alkenyl, alkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroalkyl, cycloalkyl, heterocyclyl, or heterocyclylalkyl; 
     R 4 , independently for each occurrence, is selected from substituted or unsubstituted alkyl, aryl, arylalkyl, heterocyclyl, oxo, —OR b , —CH 2 OR b , halo, hydroxyl, and hydroxyalkyl;
         R b  is substituted or unsubstituted alkyl, aryl, arylalkyl, or heterocyclyl;       

     p is 0, 1, or 2; 
     R 6  is hydrogen or substituted or unsubstituted alkyl; 
     R 7  is hydrogen or alkyl; and 
     R 9  is hydrogen or alkyl. 
     In certain embodiments: 
     R 1  and R 2  are each independently H or substituted or unsubstituted alkyl; 
     R 4  for each occurrence is hydroxyl; 
     p is 1; 
     R 6  is alkyl optionally substituted with one occurrence of —C(═O)NH 2 ; and 
     R 9  is hydrogen. 
     In certain embodiments, R 1  is substituted or unsubstituted alkyl, such as 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VII-1L) 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (VII-1D) 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 2  is H. 
     In certain embodiments, p is 1 and R 4  is hydroxyl. 
     In certain embodiments, the compound has the structure of formula (VII-4La): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VII-4Lb): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VII-4Da): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VII-4Db): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 6  is alkyl substituted with one occurrence of —C(═O)NH 2 , such as 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VII-6L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (VII-6D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 9  is —H. 
     In certain embodiments, R 7  is (C 1 -C 10 )alkyl, such as 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VII-7L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula (VII-7D): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound has the structure of formula (VII-10L): 
     
       
         
         
             
             
         
       
     
     Alternatively, the compound may have the structure of formula VII-10D): 
     
       
         
         
             
             
         
       
     
     The invention also provides a salt of a compound represented by Formula 8: 
     [Formula 8]; and 
     a salt of a compound represented by Formula 10: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, the compound may be a prodrug, e.g., wherein a hydroxyl in the parent compound is presented as an ester or a carbonate, a carboxylic acid present in the parent compound is presented as an ester, or an amino group is presented as an amide. In certain such embodiments, the prodrug is metabolized to the active parent compound in vivo (e.g., the ester is hydrolyzed to the corresponding hydroxyl or carboxylic acid). 
     In certain embodiments, compounds of the invention may be racemic. In certain embodiments, compounds of the invention may be enriched in one enantiomer. For example, a compound of the invention may have greater than 30% ee, 40% ee, 50% ee, 60% ee, 70% ee, 80% ee, 90% ee, or even 95% or greater ee. 
     The compounds of the invention have more than one stereocenter. Accordingly, the compounds of the invention may be enriched in one or more diastereomers. For example, a compound of the invention may have greater than 30% de, 40% de, 50% de, 60% de, 70% de, 80% de, 90% de, or even 95% or greater de. In certain embodiments, the compounds of the invention have substantially one isomeric configuration at one or more stereogenic centers, and have multiple isomeric configurations at the remaining stereogenic centers. 
     In certain embodiments, the enantiomeric excess of a given stereocenter in the compound is at least 40% ee, 50% ee, 60% ee, 70% ee, 80% ee, 90% ee, 92% ee, 94% ee, 95% ee, 96% ee, 98% ee or greater ee. 
     As used herein, single bonds drawn without stereochemistry do not indicate the stereochemistry of the compound. The compound of formula (I) provides an example of a compound for which no stereochemistry is indicated. 
     As used herein, hashed or bolded wedge bonds indicate absolute stereochemical configuration. 
     In certain embodiments, a therapeutic preparation of the compound of the invention may be enriched to provide predominantly one enantiomer of a compound. An enantiomerically enriched mixture may comprise, for example, at least 60 mol percent of one enantiomer, or more preferably at least 75, 90, 95, or even 99 mol percent. In certain embodiments, the compound enriched in one enantiomer is substantially free of the other enantiomer, wherein substantially free means that the substance in question makes up less than 10%, or less than 5%, or less than 4%, or less than 3%, or less than 2%, or less than 1% as compared to the amount of the other enantiomer, e.g., in the composition or compound mixture. For example, if a composition or compound mixture contains 98 grams of a first enantiomer and 2 grams of a second enantiomer, it would be said to contain 98 mol percent of the first enantiomer and only 2% of the second enantiomer. 
     In certain embodiments, a therapeutic preparation may be enriched to provide predominantly one diastereomer of the compound of the invention. A diastereomerically enriched mixture may comprise, for example, at least 60 mol percent of one diastereomer, or more preferably at least 75, 90, 95, or even 99 mol percent. 
     Methods of Treatment 
     In certain aspect, provided herein are methods of preventing or treating an IL-1α, IL-1β, IL-2, IL-6, IL-8, IL-10, TNF-α, MMP3, CCL-2, CCL-3, CCL-4, Fas, TIMP-1, or a Th1, Th2, and/or Th17 derived cytokine or proinflammatory chemokine mediated disease or disorder in a subject, comprising administering to the subject a compound disclosed herein. 
     In some embodiments, the subject has elevated levels of IL-1α, IL-1β, IL-2, IL-6, IL-8, IL-10, TNF-α, MMP3, CCL-2, CCL-3, CCL-4, Fas, TIMP-1, or a Th1, Th2, and/or Th17 derived cytokine or proinflammatory chemokine. 
     In some embodiments, the IL-1α, IL-1β, IL-2, IL-6, IL-8, IL-10, TNF-α, MMP3, CCL-2, CCL-3, CCL-4, Fas, TIMP-1, or a Th1, Th2, and/or Th17 derived cytokine or proinflammatory chemokine mediated disease is an autoimmune disease, an inflammatory disease, or a cancer, such as Acute posterior multifocal placoid pigment epitheliopathy (APMPPE), Agammaglobulinemia, Alopecia Areata, Amyloidosis, Amyotrophic lateral sclerosis (ALS), Aniridia, Ankylosing spondylitis, Anti-GBM/Anti-TBM nephritis, Antiphospholipid syndrome, Autoimmune hepatitis, Autoimmune inner ear disease, Autoimmune Lymphoproliferative Syndrome, Atopic dermatitis, Asthma, Behçet&#39;s Disease, Best Disease, Birdshot Chorioretinopathy, Blepharitis, Bronchiolitis, Cancer (Chondrosarcoma, Cervical, Breast, Lung), Capillary Leak Syndrome, Castleman disease, Celiac disease, Chagas disease, Chalazia and Stye, Chandler&#39;s syndrome, Cholesteatoma of Middle Ear, Choroideremia, Chronic recurrent multifocal osteomyelitis, Cogan&#39;s syndrome, Collagen Induced Arthritis (CIA), Cold agglutinin disease, Cone Rod Dystrophies, Conjunctivitis, Corneal Wound Healing, CREST syndrome, Crohn&#39;s disease, Dermatomyositis, Devic&#39;s disease (neuromyelitis optica), Discoid lupus, Dry Eye Disease (DED), Dry macular degeneration (Dry AMD), Endometriosis, Eosinophilic esophagitis, Eosinophilic fasciitis, Essential Iris Atrophy, Evan&#39;s syndrome, Farmer&#39;s Lung, Fibromyalgia, Giant cell arteritis, Giant cell myocarditis, Giant Papillary Conjunctivitis, Glomerulonephritis, Goodpasture&#39;s syndrome, Graft-Versus-Host Disease, Granulomatosis with polyangiitis, Graves&#39; disease, Guillain-Barre syndrome, Gyrate Atrophy, Hashimoto&#39;s thyroiditis, Hemolytic anemia, Henoch-Schonlein purpura, Hypogammaglobulinemia, Hypoproliferative anemia, IgA Nephropathy, Inclusion body myositis, Interstitial cystitis, Inflammatory Bowel Disease, Iritis, Irritant Dermatitis, Juvenile arthritis, Juvenile/Type 1 Diabetes, Juvenile macular degeneration, Juvenile myositis, Juvenile X-linked Retinoschisis, Kawasaki syndrome, Keratitis, Keratoconjunctivitis sicca (Dry Eye), Late-Onset Retinal Degeneration (L-ORD), Lichen planus, Lichen sclerosus, Lupus (SLE), Macular Edema, Meniere&#39;s disease, Multiple sclerosis, Myasthenia gravis, Microscopic polyangiitis, Neuropathic Corneal Pain, Neurotropic Keratitis, Ocular Allergy, Ocular Inflammation (uveitis), Ocular Pain, Ocular Neurodegeneration, Optic Nerve Atrophy, Optic neuritis, Oral Submucous Fibrosis, Osteroarthritis (OA), Osteoporosis, Parkinson&#39;s disease, Pars Planitis, Pemphigus, Photokeratitis, Polyarteritis nodosa, Polymyalgia rheumatica, Polymyositis, Primary biliary cirrhosis, Primary sclerosing cholangitis, Psoriasis, Psoriatic arthritis, Rheumatic fever, Rheumatoid arthritis, Sarcoidosis, Scleritis, Sjogren&#39;s syndrome, Stargardt Disease, Stickler Syndrome, Temporal arteritis/Giant cell arteritis, Thyroid Eye Disease, Trachoma, Transverse myelitis, Trichiasis, Ulcerative colitis, Usher Syndrome, Uveitis, Vasculitis, Vitiligo, Viral myocarditis, or Wegener&#39;s granulomatosis (Granulomatosis with Polyangiitis (GPA)), Wet macular degeneration, or Wound Healing. 
     In some preferred embodiments, the IL-1α, IL-1, IL-2, IL-6, IL-8, IL-10, TNF-α, MMP3, CCL-2, CCL-3, CCL-4, Fas and/or TIMP-1 mediated disease is an autoimmune disease, an inflammatory disease, or a cancer, such as Acute posterior multifocal placoid pigment epitheliopathy (APMPPE), Agammaglobulinemia, Alopecia Areata, Amyloidosis, Amyotrophic lateral sclerosis (ALS), Aniridia, Ankylosing spondylitis, Anti-GBM/Anti-TBM nephritis, Antiphospholipid syndrome, Autoimmune hepatitis, Autoimmune inner ear disease, Autoimmune Lymphoproliferative Syndrome, Atopic dermatitis, Asthma, Behçet&#39;s Disease, Best Disease, Birdshot Chorioretinopathy, Blepharitis, Bronchiolitis, Cancer (Chondrosarcoma, Cervical, Breast, Lung), Capillary Leak Syndrome, Castleman disease, Celiac disease, Chagas disease, Chalazia and Stye, Chandler&#39;s syndrome, Cholesteatoma of Middle Ear, Choroideremia, Chronic recurrent multifocal osteomyelitis, Cogan&#39;s syndrome, Collagen Induced Arthritis (CIA), Cold agglutinin disease, Cone Rod Dystrophies, Conjunctivitis, Corneal Wound Healing, CREST syndrome, Crohn&#39;s disease, Dermatomyositis, Devic&#39;s disease (neuromyelitis optica), Discoid lupus, Dry macular degeneration (Dry AMD), Endometriosis, Eosinophilic esophagitis, Eosinophilic fasciitis, Essential Iris Atrophy, Evan&#39;s syndrome, Farmer&#39;s Lung, Fibromyalgia, Giant cell arteritis, Giant cell myocarditis, Giant Papillary Conjunctivitis, Glomerulonephritis, Goodpasture&#39;s syndrome, Graft-Versus-Host Disease, Granulomatosis with polyangiitis, Graves&#39; disease, Guillain-Barre syndrome, Gyrate Atrophy, Hashimoto&#39;s thyroiditis, Hemolytic anemia, Henoch-Schonlein purpura, Hypogammaglobulinemia, Hypoproliferative anemia, IgA Nephropathy, Inclusion body myositis, Interstitial cystitis, Inflammatory Bowel Disease, Iritis, Irritant Dermatitis, Juvenile arthritis, Juvenile/Type 1 Diabetes, Juvenile macular degeneration, Juvenile myositis, Juvenile X-linked Retinoschisis, Kawasaki syndrome, Keratitis, Late-Onset Retinal Degeneration (L-ORD), Lichen planus, Lichen sclerosus, Lupus (SLE), Macular Edema, Meniere&#39;s disease, Multiple sclerosis, Myasthenia gravis, Microscopic polyangiitis, Neuropathic Corneal Pain, Neurotropic Keratitis, Ocular Allergy, Ocular Inflammation (uveitis), Ocular Pain, Ocular Neurodegeneration, Optic Nerve Atrophy, Optic neuritis, Oral Submucous Fibrosis, Osteroarthritis (OA), Osteoporosis, Parkinson&#39;s disease, Pars Planitis, Pemphigus, Photokeratitis, Polyarteritis nodosa, Polymyalgia rheumatica, Polymyositis, Primary biliary cirrhosis, Primary sclerosing cholangitis, Psoriasis, Psoriatic arthritis, Rheumatic fever, Rheumatoid arthritis, Sarcoidosis, Scleritis, Sjogren&#39;s syndrome, Stargardt Disease, Stickler Syndrome, Temporal arteritis/Giant cell arteritis, Thyroid Eye Disease, Trachoma, Transverse myelitis, Trichiasis, Ulcerative colitis, Usher Syndrome, Uveitis, Vasculitis, Vitiligo, Viral myocarditis, or Wegener&#39;s granulomatosis (Granulomatosis with Polyangiitis (GPA)), Wound Healing. 
     In some preferred embodiments, the disease or disorder is Dry Eye Disease (DED), Inflammatory Bowel Disease, Keratoconjunctivitis sicca (Dry Eye), Osteoporosis, or Rheumatoid arthritis. 
     In further preferred embodiments, the disease or disorder is Inflammatory Bowel Disease. 
     In further preferred embodiments, the disease or disorder is Keratoconjunctivitis sicca (Dry Eye). 
     In further preferred embodiments, the disease or disorder is Osteoporosis. 
     In further preferred embodiments, the disease or disorder is Rheumatoid arthritis. 
     In further preferred embodiments, the disease or disorder is Dry Eye Disease (DED) 
     In a further aspect, provided are methods of reducing production of IL-1α, IL-1β, IL-2, IL-6, IL-8, IL-10, TNF-α, MMP3, CCL-2, CCL-3, CCL-4, Fas, TIMP-1, or a Th1, Th2, and/or Th17 derived cytokine or proinflammatory chemokine in cells of a subject, comprising administering to a subject a compound disclosed herein. 
     In some embodiments, administering the compound reduces the cytokine and/or chemokine levels by at least 30%, at least 50%, or at least 70% compared to the untreated control. 
     In yet another aspect, provided are methods of reducing NF-κB transcription activity in cells of a subject, comprising administering to a subject a compound disclosed herein. 
     In some embodiments, the subject is a mammal, such as a mouse or a human, preferably a human. 
     In another aspect, provided are methods of preventing or treating Keratoconjunctivitis sicca (Dry Eye) in a subject, comprising administering to the subject a compound disclosed herein. 
     In yet another aspect, provided are methods of preventing or treating Dry Eye Disease (DED) in a subject, comprising administering to the subject a compound disclosed herein. 
     Pharmaceutical Compositions 
     In certain embodiments, the invention provides a pharmaceutical composition comprising a salt or compound of the invention, or a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable carrier. 
     In certain embodiments, the pharmaceutical composition is formulated for topical administration to the eye, e.g., as eye drops. 
     In certain embodiments at least 50%, 60%, 70%, 80%, or 90% of the compound is present as a salt. Preferably, at least 95% of the compound is present as a salt. Even more preferably, at least 99% of the compound is present as a salt. 
     In certain embodiments, the present invention provides a pharmaceutical preparation suitable for use in a human patient, comprising any salt or compound of the invention, and one or more pharmaceutically acceptable excipients. In certain embodiments, the pharmaceutical preparations may be for use in treating or preventing a condition or disease as described herein. In certain embodiments, the pharmaceutical preparations have a low enough pyrogen activity to be suitable for use in a human patient. 
     One embodiment of the present invention provides a pharmaceutical kit comprising a salt or compound of the invention, or a pharmaceutically acceptable salt thereof, and optionally directions on how to administer the compound. 
     The compositions and methods of the present invention may be utilized to treat an individual in need thereof. In certain embodiments, the individual is a mammal such as a human, or a non-human mammal. When administered to an animal, such as a human, the composition or the compound is preferably administered as a pharmaceutical composition comprising, for example, a compound of the invention and a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers are well known in the art and include, for example, aqueous solutions such as water or physiologically buffered saline or other solvents or vehicles such as glycols, glycerol, oils such as olive oil, or injectable organic esters. In certain preferred embodiments, when such pharmaceutical compositions are for human administration, particularly for invasive routes of administration (i.e., routes, such as injection or implantation, that circumvent transport or diffusion through an epithelial barrier), the aqueous solution is pyrogen-free, or substantially pyrogen-free. The excipients can be chosen, for example, to effect delayed release of an agent or to selectively target one or more cells, tissues or organs. The pharmaceutical composition can be in dosage unit form such as tablet, capsule (including sprinkle capsule and gelatin capsule), granule, lyophile for reconstitution, powder, solution, syrup, suppository, injection or the like. The composition can also be present in a transdermal delivery system, e.g., a skin patch. The composition can also be present in a solution suitable for topical administration, such as an eye drop. 
     A pharmaceutically acceptable carrier can contain physiologically acceptable agents that act, for example, to stabilize, increase solubility or to increase the absorption of a compound such as a compound of the invention. Such physiologically acceptable agents include, for example, carbohydrates, such as glucose, sucrose or dextrans, antioxidants, such as ascorbic acid or glutathione, chelating agents, low molecular weight proteins or other stabilizers or excipients. The choice of a pharmaceutically acceptable carrier, including a physiologically acceptable agent, depends, for example, on the route of administration of the composition. The preparation or pharmaceutical composition can be a selfemulsifying drug delivery system or a selfmicroemulsifying drug delivery system. The pharmaceutical composition (preparation) also can be a liposome or other polymer matrix, which can have incorporated therein, for example, a compound of the invention. Liposomes, for example, which comprise phospholipids or other lipids, are nontoxic, physiologically acceptable and metabolizable carriers that are relatively simple to make and administer. 
     The phrase “pharmaceutically acceptable” is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio. 
     The phrase “pharmaceutically acceptable carrier” as used herein means a pharmaceutically acceptable material, composition or vehicle, such as a liquid or solid filler, diluent, excipient, solvent or encapsulating material. Each carrier must be “acceptable” in the sense of being compatible with the other ingredients of the formulation and not injurious to the patient. Some examples of materials which can serve as pharmaceutically acceptable carriers include: (1) sugars, such as lactose, glucose and sucrose; (2) starches, such as corn starch and potato starch; (3) cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; (4) powdered tragacanth; (5) malt; (6) gelatin; (7) talc; (8) excipients, such as cocoa butter and suppository waxes; (9) oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; (10) glycols, such as propylene glycol; (11) polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; (12) esters, such as ethyl oleate and ethyl laurate; (13) agar; (14) buffering agents, such as magnesium hydroxide and aluminum hydroxide; (15) alginic acid; (16) pyrogen-free water; (17) isotonic saline; (18) Ringer&#39;s solution; (19) ethyl alcohol; (20) phosphate buffer solutions; and (21) other non-toxic compatible substances employed in pharmaceutical formulations. 
     A pharmaceutical composition (preparation) can be administered to a subject by any of a number of routes of administration including, for example, orally (for example, drenches as in aqueous or non-aqueous solutions or suspensions, tablets, capsules (including sprinkle capsules and gelatin capsules), boluses, powders, granules, pastes for application to the tongue); absorption through the oral mucosa (e.g., sublingually); anally, rectally or vaginally (for example, as a pessary, cream or foam); parenterally (including intramuscularly, intravenously, subcutaneously or intrathecally as, for example, a sterile solution or suspension); nasally; intraperitoneally; subcutaneously; transdermally (for example as a patch applied to the skin); and topically (for example, as a cream, ointment or spray applied to the skin, or as an eye drop). The compound may also be formulated for inhalation. In certain embodiments, a compound may be simply dissolved or suspended in sterile water. Details of appropriate routes of administration and compositions suitable for same can be found in, for example, U.S. Pat. Nos. 6,110,973, 5,763,493, 5,731,000, 5,541,231, 5,427,798, 5,358,970 and 4,172,896, as well as in patents cited therein. 
     The formulations may conveniently be presented in unit dosage form and may be prepared by any methods well known in the art of pharmacy. The amount of active ingredient which can be combined with a carrier material to produce a single dosage form will vary depending upon the host being treated, the particular mode of administration. The amount of active ingredient that can be combined with a carrier material to produce a single dosage form will generally be that amount of the compound which produces a therapeutic effect. Generally, out of one hundred percent, this amount will range from about 1 percent to about ninety-nine percent of active ingredient, preferably from about 5 percent to about 70 percent, most preferably from about 10 percent to about 30 percent. 
     Methods of preparing these formulations or compositions include the step of bringing into association an active compound, such as a compound of the invention, with the carrier and, optionally, one or more accessory ingredients. In general, the formulations are prepared by uniformly and intimately bringing into association a compound of the present invention with liquid carriers, or finely divided solid carriers, or both, and then, if necessary, shaping the product. 
     Formulations of the invention suitable for oral administration may be in the form of capsules (including sprinkle capsules and gelatin capsules), cachets, pills, tablets, lozenges (using a flavored basis, usually sucrose and acacia or tragacanth), lyophile, powders, granules, or as a solution or a suspension in an aqueous or non-aqueous liquid, or as an oil-in-water or water-in-oil liquid emulsion, or as an elixir or syrup, or as pastilles (using an inert base, such as gelatin and glycerin, or sucrose and acacia) and/or as mouth washes and the like, each containing a predetermined amount of a compound of the present invention as an active ingredient. Compositions or compounds may also be administered as a bolus, electuary or paste. 
     To prepare solid dosage forms for oral administration (capsules (including sprinkle capsules and gelatin capsules), tablets, pills, dragees, powders, granules and the like), the active ingredient is mixed with one or more pharmaceutically acceptable carriers, such as sodium citrate or dicalcium phosphate, and/or any of the following: (1) fillers or extenders, such as starches, lactose, sucrose, glucose, mannitol, and/or silicic acid; (2) binders, such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinyl pyrrolidone, sucrose and/or acacia; (3) humectants, such as glycerol; (4) disintegrating agents, such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate; (5) solution retarding agents, such as paraffin; (6) absorption accelerators, such as quaternary ammonium compounds; (7) wetting agents, such as, for example, cetyl alcohol and glycerol monostearate; (8) absorbents, such as kaolin and bentonite clay; (9) lubricants, such a talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof, (10) complexing agents, such as, modified and unmodified cyclodextrins; and (11) coloring agents. In the case of capsules (including sprinkle capsules and gelatin capsules), tablets and pills, the pharmaceutical compositions may also comprise buffering agents. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugars, as well as high molecular weight polyethylene glycols and the like. 
     A tablet may be made by compression or molding, optionally with one or more accessory ingredients. Compressed tablets may be prepared using binder (for example, gelatin or hydroxypropylmethyl cellulose), lubricant, inert diluent, preservative, disintegrant (for example, sodium starch glycolate or cross-linked sodium carboxymethyl cellulose), surface-active or dispersing agent. Molded tablets may be made by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. 
     The tablets, and other solid dosage forms of the pharmaceutical compositions, such as dragees, capsules (including sprinkle capsules and gelatin capsules), pills and granules, may optionally be scored or prepared with coatings and shells, such as enteric coatings and other coatings well known in the pharmaceutical-formulating art. They may also be formulated so as to provide slow or controlled release of the active ingredient therein using, for example, hydroxypropylmethyl cellulose in varying proportions to provide the desired release profile, other polymer matrices, liposomes and/or microspheres. They may be sterilized by, for example, filtration through a bacteria-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions that can be dissolved in sterile water, or some other sterile injectable medium immediately before use. These compositions may also optionally contain opacifying agents and may be of a composition that they release the active ingredient(s) only, or preferentially, in a certain portion of the gastrointestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. The active ingredient can also be in micro-encapsulated form, if appropriate, with one or more of the above-described excipients. 
     Liquid dosage forms useful for oral administration include pharmaceutically acceptable emulsions, lyophiles for reconstitution, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active ingredient, the liquid dosage forms may contain inert diluents commonly used in the art, such as, for example, water or other solvents, cyclodextrins and derivatives thereof, solubilizing agents and emulsifiers, such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils (in particular, cottonseed, groundnut, corn, germ (e.g., wheat germ), olive, castor and sesame oils), glycerol, tetrahydrofuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. 
     Besides inert diluents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, coloring, perfuming and preservative agents. 
     Suspensions, in addition to the active compounds, may contain suspending agents as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar and tragacanth, and mixtures thereof. 
     Formulations of the pharmaceutical compositions for rectal, vaginal, or urethral administration may be presented as a suppository, which may be prepared by mixing one or more active compounds with one or more suitable nonirritating excipients or carriers comprising, for example, cocoa butter, polyethylene glycol, a suppository wax or a salicylate, and which is solid at room temperature, but liquid at body temperature and, therefore, will melt in the rectum or vaginal cavity and release the active compound. 
     Formulations of the pharmaceutical compositions for administration to the mouth may be presented as a mouthwash, or an oral spray, or an oral ointment. 
     Alternatively or additionally, compositions can be formulated for delivery via a catheter, stent, wire, or other intraluminal device. Delivery via such devices may be especially useful for delivery to the bladder, urethra, ureter, rectum, or intestine. 
     Formulations which are suitable for vaginal administration also include pessaries, tampons, creams, gels, pastes, foams or spray formulations containing such carriers as are known in the art to be appropriate. 
     Dosage forms for the topical or transdermal administration include powders, sprays, ointments, pastes, creams, lotions, gels, solutions, patches and inhalants. The active compound may be mixed under sterile conditions with a pharmaceutically acceptable carrier, and with any preservatives, buffers, or propellants that may be required. 
     The ointments, pastes, creams and gels may contain, in addition to an active compound, excipients, such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof. 
     Powders and sprays can contain, in addition to an active compound, excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and polyamide powder, or mixtures of these substances. Sprays can additionally contain customary propellants, such as chlorofluorohydrocarbons and volatile unsubstituted hydrocarbons, such as butane and propane. 
     Transdermal patches have the added advantage of providing controlled delivery of a compound of the present invention to the body. Such dosage forms can be made by dissolving or dispersing the active compound in the proper medium. Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate of such flux can be controlled by either providing a rate controlling membrane or dispersing the compound in a polymer matrix or gel. 
     Ophthalmic formulations, eye ointments, powders, solutions and the like, are also contemplated as being within the scope of this invention. Exemplary ophthalmic formulations are described in U.S. Publication Nos. 2005/0080056, 2005/0059744, 2005/0031697 and 2005/004074 and U.S. Pat. No. 6,583,124, the contents of which are incorporated herein by reference. If desired, liquid ophthalmic formulations have properties similar to that of lacrimal fluids, aqueous humor or vitreous humor or are compatable with such fluids. A preferred route of administration is local administration (e.g., topical administration, such as eye drops, or administration via an implant). 
     The phrases “parenteral administration” and “administered parenterally” as used herein means modes of administration other than enteral and topical administration, usually by injection, and includes, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal and intrasternal injection and infusion. 
     Pharmaceutical compositions suitable for parenteral administration comprise one or more active compounds in combination with one or more pharmaceutically acceptable sterile isotonic aqueous or nonaqueous solutions, dispersions, suspensions or emulsions, or sterile powders which may be reconstituted into sterile injectable solutions or dispersions just prior to use, which may contain antioxidants, buffers, bacteriostats, solutes which render the formulation isotonic with the blood of the intended recipient or suspending or thickening agents. 
     Examples of suitable aqueous and nonaqueous carriers that may be employed in the pharmaceutical compositions of the invention include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like), and suitable mixtures thereof, vegetable oils, such as olive oil, and injectable organic esters, such as ethyl oleate. Proper fluidity can be maintained, for example, by the use of coating materials, such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants. 
     These compositions may also contain adjuvants such as preservatives, wetting agents, emulsifying agents and dispersing agents. Prevention of the action of microorganisms may be ensured by the inclusion of various antibacterial and antifungal agents, for example, paraben, chlorobutanol, phenol sorbic acid, and the like. It may also be desirable to include isotonic agents, such as sugars, sodium chloride, and the like into the compositions. In addition, prolonged absorption of the injectable pharmaceutical form may be brought about by the inclusion of agents that delay absorption such as aluminum monostearate and gelatin. 
     In some cases, in order to prolong the effect of a drug, it is desirable to slow the absorption of the drug from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material having poor water solubility. The rate of absorption of the drug then depends upon its rate of dissolution, which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered drug form is accomplished by dissolving or suspending the drug in an oil vehicle. 
     Injectable depot forms are made by forming microencapsulated matrices of the subject compounds in biodegradable polymers such as polylactide-polyglycolide. Depending on the ratio of drug to polymer, and the nature of the particular polymer employed, the rate of drug release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations are also prepared by entrapping the drug in liposomes or microemulsions that are compatible with body tissue. 
     For use in the methods of this invention, active compounds can be given per se or as a pharmaceutical composition containing, for example, 0.1 to 99.5% (more preferably, 0.5 to 90%) of active ingredient in combination with a pharmaceutically acceptable carrier. 
     Methods of introduction may also be provided by rechargeable or biodegradable devices. Various slow release polymeric devices have been developed and tested in vivo in recent years for the controlled delivery of drugs, including proteinaceous biopharmaceuticals. A variety of biocompatible polymers (including hydrogels), including both biodegradable and non-degradable polymers, can be used to form an implant for the sustained release of a compound at a particular target site. 
     Actual dosage levels of the active ingredients in the pharmaceutical compositions may be varied so as to obtain an amount of the active ingredient that is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient. 
     The selected dosage level will depend upon a variety of factors including the activity of the particular compound or combination of compounds employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion of the particular compound(s) being employed, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular compound(s) employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors well known in the medical arts. 
     A physician or veterinarian having ordinary skill in the art can readily determine and prescribe the therapeutically effective amount of the pharmaceutical composition required. For example, the physician or veterinarian could start doses of the pharmaceutical composition or compound at levels lower than that required in order to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved. By “therapeutically effective amount” is meant the concentration of a compound that is sufficient to elicit the desired therapeutic effect. It is generally understood that the effective amount of the compound will vary according to the weight, sex, age, and medical history of the subject. Other factors which influence the effective amount may include, but are not limited to, the severity of the patient&#39;s condition, the disorder being treated, the stability of the compound, and, if desired, another type of therapeutic agent being administered with the compound of the invention. A larger total dose can be delivered by multiple administrations of the agent. Methods to determine efficacy and dosage are known to those skilled in the art (Isselbacher et al. (1996) Harrison&#39;s Principles of Internal Medicine 13 ed., 1814-1882, herein incorporated by reference). 
     In general, a suitable daily dose of an active compound used in the compositions and methods of the invention will be that amount of the compound that is the lowest dose effective to produce a therapeutic effect. Such an effective dose will generally depend upon the factors described above. 
     If desired, the effective daily dose of the active compound may be administered as one, two, three, four, five, six or more sub-doses administered separately at appropriate intervals throughout the day, optionally, in unit dosage forms. In certain embodiments of the present invention, the active compound may be administered two or three times daily. In preferred embodiments, the active compound will be administered once daily. 
     The patient receiving this treatment is any animal in need, including primates, in particular humans, and other mammals such as equines, cattle, swine and sheep; and poultry and pets in general. 
     In certain embodiments, compounds of the invention may be used alone or conjointly administered with another type of therapeutic agent. As used herein, the phrase “conjoint administration” refers to any form of administration of two or more different therapeutic compounds such that the second compound is administered while the previously administered therapeutic compound is still effective in the body (e.g., the two compounds are simultaneously effective in the patient, which may include synergistic effects of the two compounds). For example, the different therapeutic compounds can be administered either in the same formulation or in a separate formulation, either concomitantly or sequentially. In certain embodiments, the different therapeutic compounds can be administered within one hour, 12 hours, 24 hours, 36 hours, 48 hours, 72 hours, or a week of one another. Thus, an individual who receives such treatment can benefit from a combined effect of different therapeutic compounds. 
     In certain embodiments, conjoint administration of compounds of the invention with one or more additional therapeutic agent(s) provides improved efficacy relative to each individual administration of the compound of the invention (e.g., a compound of formula I, V, VI, or VII) or the one or more additional therapeutic agent(s). In certain such embodiments, the conjoint administration provides an additive effect, wherein an additive effect refers to the sum of each of the effects of individual administration of the compound of the invention and the one or more additional therapeutic agent(s). 
     This invention includes the use of pharmaceutically acceptable salts of compounds of the invention in the compositions and methods of the present invention. The term “pharmaceutically acceptable salt” as used herein includes salts derived from inorganic or organic acids including, for example, hydrochloric, hydrobromic, sulfuric, nitric, perchloric, phosphoric, formic, acetic, lactic, maleic, fumaric, succinic, tartaric, glycolic, salicylic, citric, methanesulfonic, benzenesulfonic, benzoic, malonic, trifluoroacetic, trichloroacetic, naphthalene-2-sulfonic, oxalic, mandelic and other acids. Pharmaceutically acceptable salt forms can include forms wherein the ratio of molecules comprising the salt is not 1:1. For example, the salt may comprise more than one inorganic or organic acid molecule per molecule of base, such as two hydrochloric acid molecules per molecule of compound of Formula I, V, VI, or VII. As another example, the salt may comprise less than one inorganic or organic acid molecule per molecule of base, such as two molecules of compound of Formula I, V, VI, or VII per molecule of tartaric acid. 
     In further embodiments, contemplated salts of the invention include, but are not limited to, alkyl, dialkyl, trialkyl or tetra-alkyl ammonium salts. In certain embodiments, contemplated salts of the invention include, but are not limited to, L-arginine, benenthamine, benzathine, betaine, calcium hydroxide, choline, deanol, diethanolamine, diethylamine, 2-(diethylamino)ethanol, ethanolamine, ethylenediamine, N-methylglucamine, hydrabamine, 1H-imidazole, lithium, L-lysine, magnesium, 4-(2-hydroxyethyl)morpholine, piperazine, potassium, 1-(2-hydroxyethyl)pyrrolidine, sodium, triethanolamine, tromethamine, and zinc salts. In certain embodiments, contemplated salts of the invention include, but are not limited to, Na, Ca, K, Mg, Zn or other metal salts. 
     The pharmaceutically acceptable acid addition salts can also exist as various solvates, such as with water, methanol, ethanol, dimethylformamide, and the like. Mixtures of such solvates can also be prepared. The source of such solvate can be from the solvent of crystallization, inherent in the solvent of preparation or crystallization, or adventitious to such solvent. 
     Wetting agents, emulsifiers and lubricants, such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, release agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the compositions. 
     Examples of pharmaceutically acceptable antioxidants include: (1) water-soluble antioxidants, such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulfite, sodium sulfite and the like; (2) oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), lecithin, propyl gallate, alpha-tocopherol, and the like; and (3) metal-chelating agents, such as citric acid, ethylenediamine tetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid, and the like. 
     EXAMPLES 
     Example 1: Preparation of YDE-093 to YDE-107 Peptides 
     YDE peptides (YDE-093 to YDE-107), derivatives of the amino acid sequence of the YDE-011 (WO2018/225961), were obtained through the C-terminal modification or fragmentation of YDE-011. 
     
       
         
         
             
             
         
       
     
     In order to prepare C-terminal modified peptide, Fmoc solid-phase peptide synthesis (SPPS) was conducted, based on a standard procedure described by prior invention WO2018/225961 and further a C-terminal amidation reaction was carried out. 
     YDE-093 to YDE-107 peptides were synthesized by ANYGEN (Gwangju, Korea) and IRBM (Rome, Italy) by substituting one or more different amino acid residues into the peptide (2S,4R)hydroxyproline-GQLGLAGPK(NH-PEG1-NH-Boc) (Table 1). 
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 Solubility 
               
               
                   
                 Lot 
                   
                   
                   
                 Purity 
                 Amount 
                 in Water 
               
               
                 # 
                 No. 
                 Peptide 
                 Structure / Sequence 
                 M. W. 
                 (%) 
                 (mg) 
                 (mg/mL) 
               
               
                   
               
             
            
               
                  1 
                 K182 143 
                 YDE- 093 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 1139.4 
                 99.1 
                 10 
                 10.0 
               
               
                   
               
               
                   
                   
                   
                 O*GQLGLAGPK(NH-PEG1-NH-Boc) 
                   
                   
                   
                   
               
               
                   
               
               
                  2 
                 K182 472 
                 YDE- 094 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                  672.7 
                 97.4 
                 30 
                 10.0 
               
               
                   
               
               
                   
                   
                   
                 O*GQDGLA 
                   
                   
                   
                   
               
               
                   
               
               
                  3 
                 I-0417 327- 001- 001 
                 YDE- 096 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 1173.4 
                 95.4 
                 10 
                 10.0 
               
               
                   
               
               
                   
                   
                   
                 O*GQLGLAGPK(NH-PEG1-NH-Cbz) 
                   
                   
                   
                   
               
               
                   
               
               
                  4 
                 K190 638 
                 YDE- 100 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                  824.9 
                 98.7 
                 20 
                 10.0 
               
               
                   
               
               
                   
                   
                   
                 O*GQLGLAGP 
                   
                   
                   
                   
               
               
                   
               
               
                  5 
                 K190 639 
                 YDE- 101 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                  852.0 
                 96.7 
                 20 
                 10.0 
               
               
                   
               
               
                   
                   
                   
                 O*GQLGLAGP(dimethyl) 
                   
                   
                   
                   
               
               
                   
               
               
                  6 
                 K190 640 
                 YDE- 102 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                  880.1 
                 97.2 
                 20 
                 10.0 
               
               
                   
               
               
                   
                   
                   
                 O*GQLGLAGP(diethyl) 
                   
                   
                   
                   
               
               
                   
               
               
                  7 
                 K190 641 
                 YDE- 103 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                  864.0 
                 97.9 
                 20 
                 10.0 
               
               
                   
               
               
                   
                   
                   
                 O*GQLGLAGP(azetidine) 
                   
                   
                   
                   
               
               
                   
               
               
                  8 
                 K190 643 
                 YDE- 105 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                  866.0 
                 97.4 
                 20 
                 10.0 
               
               
                   
               
               
                   
                   
                   
                 O*GQLGLAGP(methylethyl) 
                   
                   
                   
                   
               
               
                   
               
               
                  9 
                 K190 644 
                 YDE- 106 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                  878.0 
                 97.2 
                 20 
                 10.0 
               
               
                   
               
               
                   
                   
                   
                 O*GQLGLAGP(pyrroidine) 
                   
                   
                   
                   
               
               
                   
               
               
                 10 
                 K190 645 
                 YDE- 107 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                  894.1 
                 98.2 
                 20 
                 10.0 
               
               
                   
               
               
                   
                   
                   
                 O*GQLGLAGP(isopropylethyl) 
               
               
                   
               
               
                 O* = (2S,4R)hydroxyproline 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
            
           
         
       
     
     The process for synthesizing the YDE-093 to YDE-107 peptides and the purification procedure thereof conducted by ANYGEN and IRBM are depicted below. 

 
     Analysis of YDE Peptides 
     The YDE peptides prepared in Example 1 were analyzed by HPLC. As a result, it was confirmed that the purities of YDE-093, YDE-094, YDE-096, YDE-100, YDE-101, YDE-102, YDE-103, YDE-105, YDE-106 and YDE-107 synthesized were 99.1%, 97.4%, 95.4%, 98.7%, 96.7%, 97.2%, 97.9%, 97.4%, 97.2% and 98.2%, respectively. 
     In addition, the YDE derivatives prepared in Example 1 were analyzed by Ion-Mass. As a result, it was confirmed that the molecular weights of YDE-093, YDE-094, YDE-096, YDE-100, YDE-101, YDE-102, YDE-103, YDE-105, YDE-106 and YDE-107 synthesized were 1139.0, 673.2, 1173.6, 823.9, 851.9, 880.1, 864.5, 866.1, 878.4 and 894.3, respectively. 
     Example 2: Evaluation of the Effect of Peptides on Soluble IL-6 Cytokine Release in Poly I:C Stimulated Primary Human Corneal Epithelial Cells 
     Assay protocol as shown below was used to determine the significant effect of test compounds. The data was statistically analyzed by one-way ANOVA with a Bonferroni post test comparing all the columns. The significance was represented by the p value. 

 
     Selected YDE peptides (YDE-093, -096, -100, -101, -102, -103, -104, -105, -106 and -107) were evaluated by assessing the soluble IL-6 cytokine release in poly I:C stimulated primary human corneal epithelial cells by ELISA (Biolegend, 430504). 
     Specifically, primary corneal epithelial cells (ATCC, ATCC PCS-700-010) were seeded on a 6-well culture plate containing the Corneal Epithelial Cell Basal Medium (ATCC, ATCC PCS-700-030) in the Corneal Epithelial Cell Growth Kit (ATCC, ATCC PCS-700-040) in an amount of 1.2×10 5  cells per well, which was then cultured for 24 hours under the conditions of 37° C. and 5% CO 2 . Then, cells were washed with 1×PBS and replaced with serum free medium which was then cultured for 2 hours under the conditions of 37° C. and 5% CO 2 . 
     After 2 hours, cells were treated with compounds at 6 differential concentrations (Hinokitiol/YDE peptides at 30, 10, 1, 0.1, 0.01, and 0.001 μM) with the DMSO final 0.5% and then cultured for 1 hour under the conditions of 37° C. and 5% CO 2 . 
     After 1 hour, cells were further treated with 25 μg/mL of poly I:C and cultured for 24 hours under the conditions of 37° C. and 5% CO 2 . 
     Following incubation, the supernatant was collected for IL-6 levels was assessed by Sandwich ELISA (Enzyme-linked immunosorbent assay) following manufacturer&#39;s instructions. The data was statistically analyzed by one-way ANOVA with a Bonferroni post-test comparing all the columns. The significance was represented by the p value. 
     Results &amp; Conclusion 
     Human corneal epithelial cells on stimulation with Poly I:C resulted in a significantly increase of IL-6 cytokine levels. Reference compound, Hinokitiol was found to significantly attenuate the Poly I:C stimulated IL-6 cytokine release in a dose dependent manner. 
     As shown in  FIG.  1 A- 1 D , test peptides YDE-093 and YDE-096 were found to attenuate Poly I:C induced of IL-6 cytokine release in a dose dependent manner. Test compounds YDE103, and YDE104, were found to attenuate Poly I:C induced IL-6 cytokine release in a dose dependent manner. This was corroborated by the significant attenuation of IL-6 cytokine release at the tested concentrations that included; 30 μM, 10 μM, 1 μM and 0.1 μM respectively. Test compounds YDE101, YDE102, YDE105, YDE106 and YDE107 were found to attenuate Poly I:C induced IL-6 cytokine release in a dose dependent manner. This was corroborated by the significant attenuation of IL-6 cytokine release at the tested concentrations that included; 30 μM, 10 μM, and 1 μM respectively. Test compound YDE100 was found to attenuate Poly I:C induced IL-6 cytokine release in a dose dependent manner. This was corroborated by the significant attenuation of IL-6 cytokine release at the tested concentrations that included; 30 μM, and 10 μM respectively. 
     Example 3: Evaluation of the Effect of Peptides on Cell Proliferation Using Primary Corneal Epithelial Cells 
     Study Objective 
     To determine the cell proliferation assessment of peptides using primary corneal epithelial cells. 
     Study Plan 
     
         
         
           
             Test System: HCE cells from ATCC. 
             Assay Format: 96-well plate format 
             Method of Detection: CellTiter-Glo Luminescent Cell Viability Assay (Pro mega, Cat #G7573) 
             Assay Controls: Reference compound (hEGF) and untreated cells 
             Test Samples: 57 
             Media: Corneal Epithelial Cell Basal Medium+Cell growth kit components EXPERIMENTAL DESIGN 

 
           
         
       
    
     Selected YDE peptides (YDE-012, -019, -038, -044, -045, -047, -048, -049, -050, -051, -052, -053, -054, -055, -056, -057, -058, -059, -060, -061, -062, -063, -064, -065, -066, -067, -072, -073, -074, -075, -076, -077, -078, -079, -080, -081, -082, -083, -084, -085, -086, and -087) were evaluated by assessing the effect of peptides on cell proliferation of primary corneal epithelial cells. 
     Specifically, 5000 primary corneal epithelial cells/well were seeded in a white opaque 96-well plate and incubated for 24 hrs in a 37° C. incubator supplemented with 5% CO 2 . After 24 hours, test compounds were treated at 8 different concentrations. Cells treated with compounds were incubated for 48 &amp; 72 hrs at 37° C. in a 5% CO 2  incubator. Post appropriate incubation time points, CellTiter-Glo luminescent reagent (Promega, Cat #G7573) was added to the plates and incubated at room temperature for 30 min. Luminescence signal was captured using EnVision 2104 ® Multilabel reader. Assay controls were reference compound (hEGF) and untreated cells. 
     Results &amp; Conclusion 
     The proliferation effect of YDE-038 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-038 for 48 hrs and 72 hrs. At concentrations 30, 10, 3 and 1 μM, no significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations (0.03 &amp; 0.01 μM) an increase in cell proliferation (35-40%) in 72 hrs incubation was observed. Whereas after 48 hrs incubation there was no proliferation observed, even at lower concentrations ( FIG.  2 A ). 
     The proliferation effect of YDE-044 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-044 for 48 hrs and 72 hrs. At concentrations 30 μM, no significant proliferation of cells (&lt;20% over basal) was observed. At low concentrations of YDE-044, there was proliferation of cells (20-30%). Maximum 30% proliferation was observed at the lowest concentration (0.01 μM) ( FIG.  2 B ). 
     The proliferation effect of YDE-045 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-045 for 48 hrs and 72 hrs. At concentrations 30, 10, 3 and 1 μM, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated with YDE-045 for 48 hrs or 72 hrs. However, at lower concentrations an increase in cell proliferation (35-50%) was observed ( FIG.  2 C ). 
     The proliferation effect of YDE-049 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-049 for 48 hrs and 72 hrs. There was no significant proliferation of cells when YDE-049 was incubated at 48 hrs incubation. At 30, 10 and 3 μM the peptide showed toxic effect (˜25-40%). However, at 72 hrs incubation, at concentrations 30, 10, 3 and 1 μM, no significant proliferation of cells was observed (&lt;20% over basal). At lower concentrations, an increase in cell proliferation (25-40%) was observed when YDE-049 was incubated with primary corneal epithelial cells for 72 hrs ( FIG.  2 D ). 
     The proliferation effect of YDE-053 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-053 for 48 hrs and 72 hrs. At concentrations 30, 10 and 3 μM, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations an increase in cell proliferation (40-60%) was observed ( FIG.  2 E ). 
     The proliferation effect of YDE-054 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-054 for 48 hrs and 72 hrs. No significant proliferation at 48 hrs incubation was observed. At concentrations 30, 10, 3 and 1 μM, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 72 hrs. However, at lower concentrations an increase in cell proliferation (25-40%) at 72 hrs incubation was observed ( FIG.  2 F ). 
     The proliferation effect of YDE-058 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-058 for 48 hrs and 72 hrs. No significant proliferation at 48 hrs incubation was observed. At concentrations 30 and 10 μM, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 72 hrs. However, at lower concentrations moderate increase in cell proliferation (˜25%) at 72 hrs incubation was observed ( FIG.  2 G ). 
     The proliferation effect of YDE-059 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-059 for 48 hrs and 72 hrs. At concentrations 30 μM, no significant proliferation of cells (&lt;20% over basal) was observed. Dose dependent proliferation in tested concentrations in 72 hrs incubation with high proliferation at lower dilutions and lower proliferation at high dilutions of peptides was observed. Maximum 40% proliferation resulted in the lowest concentration 0.01 μM ( FIG.  2 H ). 
     The proliferation effect of YDE-063 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-063 for 48 hrs and 72 hrs. No significant proliferation of cells in 48 hrs incubation was observed. However, at lower concentrations increase in cell proliferation (˜30%) at 72 hrs incubation was observed ( FIG.  2 I ). 
     The proliferation effect of YDE-064 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-064 for 48 hrs and 72 hrs. No significant proliferation of cells in 48 hrs. incubation was observed. However, at lower concentrations (0.03 &amp; 0.01 μM) an increase in cell proliferation (˜35%) at 72 hrs incubation time point was observed ( FIG.  2 J ). 
     The proliferation effect of YDE-065 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-065 for 48 hrs and 72 hrs. At concentrations 30, 10, 3 and 1 μM, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations an increase in cell proliferation (˜35%) at 72 hrs incubation was observed ( FIG.  2 K ). 
     The proliferation effect of YDE-066 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-066 for 48 hrs and 72 hrs. There was no significant increase in cell proliferation when the peptide was incubated with cells for 48 hrs or 72 hrs ( FIG.  2 L ). 
     The proliferation effect of YDE-067 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-067 for 48 hrs and 72 hrs. At concentrations 30, 10, 3 and 1 μM, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations (0.03 &amp; 0.01 μM), an increase in cell proliferation (35%) in 72 hrs incubation was observed. Whereas 48 hrs incubation no significant proliferation was observed even at lower concentrations ( FIG.  2 M ). 
     The proliferation effect of YDE-045 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-045 for 48 hrs and 72 hrs. At concentrations 30, 10, μM, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations (0.1, 0.03 &amp; 0.01 μM) an increase in cell proliferation (35-40%) in 72 hrs incubation was observed ( FIG.  2 N ). 
     The proliferation effect of YDE-049 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-049 for 48 hrs and 72 hrs. At all tested concentrations, no significant proliferation of cells (&lt;20% over basal) at 48 hrs was observed. Proliferation at 0.03 &amp; 0.01 concentrations at 72 hrs incubation was observed. Maximum 30% proliferation was observed at the lowest concentration (0.01 μM) ( FIG.  2 O ). 
     The proliferation effect of YDE-053 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-053 for 48 hrs and 72 hrs. At concentrations 30, 10 and 3 μM, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations an increase in cell proliferation (40-60%) was observed ( FIG.  2 P ). 
     The proliferation effect of YDE-057 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-057 for 48 hrs and 72 hrs. Significant proliferation at 48 hrs incubation with 0.1, 0.03 &amp; 0.01 μM concentrations was observed. At concentrations 30, 10, 3 and 1 μM, no significant proliferation of cells (&lt;20% over basal) observed in 48 hrs incubation was observed. However, at lower concentrations an increase in cell proliferation (40-50%) at 72 hrs incubation was observed ( FIG.  2 Q ). 
     The proliferation effect of YDE-060 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-060 for 48 hrs and 72 hrs. At concentrations 30, 10, 3, 1 and 0.3 μM, significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 72 hrs. However, at lower concentrations an increase in cell proliferation (˜40-60%) at 72 hrs incubation was observed ( FIG.  2 R ). 
     The proliferation effect of YDE-065 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-065 for 48 hrs and 72 hrs. No significant proliferation of cells (&lt;20% over basal) in 48 hrs incubation was observed. Dose dependent proliferation in tested concentrations in 72 hrs incubation with high proliferation at lower dilutions was observed. Maximum 40% proliferation resulted in the lowest concentration 0.01 μM ( FIG.  2 S ). 
     The proliferation effect of YDE-067 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-067 for 48 hrs and 72 hrs. No significant proliferation of cells in 48 hrs Incubation. However, at lower concentrations an increase in cell proliferation (˜30%) at 72 hrs incubation was observed ( FIG.  2 T ) 
     The proliferation effect of YDE-072 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-072 for 48 hrs and 72 hrs. No significant proliferation of cells in 48 hrs. Incubation except 0.03 &amp; 0.01 μM concentrations. However, at lower concentrations (0.03 &amp; 0.01 μM) an increase in cell proliferation (˜30%) at 72 hrs incubation time point was observed ( FIG.  2 U ). 
     The proliferation effect of YDE-073 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-073 for 48 hrs and 72 hrs. No significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs. However, at lower concentrations an increase in cell proliferation (˜30%) at 72 hrs incubation was observed. 
     The proliferation effect of YDE-074 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-074 for 48 hrs and 72 hrs. There was no significant increase in cell proliferation when the peptide was incubated with cells for 48 hrs or 72 hrs ( FIG.  2 W ). 
     The proliferation effect of YDE-075 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-075 for 48 hrs and 72 hrs. There was no significant increase in cell proliferation when the peptide was incubated with cells for 48 hrs. However, at lower concentrations (0.03 &amp; 0.01 μM) an increase in cell proliferation (˜30%) at 72 hrs incubation time point was observed ( FIG.  2 X ). 
     The proliferation effect of Diquas was assessed using human primary corneal epithelial cells. Cells were incubated with Diquas for 48 hrs and 72 hrs. Dose dependent increase in cell proliferation when the peptide was incubated with cells for 48 hrs or 72 hrs was observed ( FIG.  2 Y ). 
     The proliferation effect of YDE-053 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-053 for 48 hrs and 72 hrs. An increased proliferation of cells up to 10M (&gt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations no significant proliferation (i.e from 0.1 nM to 0.0001 nM) was observed ( FIG.  2 Z ). 
     The proliferation effect of YDE-067 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-067 for 48 hrs and 72 hrs. No significant proliferation of cells with 48 hrs incubation of compound was observed. Whereas in 72 hrs an increased proliferation up to 10 nM was observed. However, at lower concentrations a decrease in cell proliferation (0.1 nM to 0.0001 nM) was observed ( FIG.  2 AA ). 
     The proliferation effect of Diquas was assessed using human primary corneal epithelial cells. Cells were incubated with Diquas for 48 hrs and 72 hrs. Dose dependent increase in cell proliferation when the peptide was incubated with cells for 48 hrs or 72 hrs was observed. There is no significant proliferation observed from 1 nM to 0.0001 nM ( FIG.  2 AB ). 
     The proliferation effect of YDE-045 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-045 for 48 hrs and 72 hrs. An increased proliferation of cells up to 10 nM (˜35% over basal) was observed. This effect was observed in 48 hrs incubation. In 72 hrs incubation concentration dependent increase proliferation observed up to 10 nM. However, at lower concentrations no significant proliferation was observed (i.e. 0.001 nM &amp; 0.0001 nM) ( FIG.  2 AC ). 
     The proliferation effect of YDE-053 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-053 for 48 hrs and 72 hrs. An increased proliferation of cells up to 10 nM (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations no significant proliferation was observed (i.e. 0.1 nM to 0.0001 nM) ( FIG.  2 AD ). 
     The proliferation effect of YDE-054 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-054 for 48 hrs and 72 hrs. An increased proliferation of cells up to 0.1 nM (˜30% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs, whereas at 72 hrs increased proliferation up to 10 nM with 40% proliferation. At higher concentration (1000 nM) there was no significant proliferation was observed ( FIG.  2 AE ). 
     The proliferation effect of YDE-057 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-057 for 48 hrs and 72 hrs. An increased proliferation of cells up to 10 nM was observed. This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations no significant proliferation was observed (i.e. 0.1 nM to 0.0001 nM) ( FIG.  2 AF ). 
     The proliferation effect of YDE-060 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-060 for 48 hrs and 72 hrs. Significant proliferation of cells in 48 hrs as well as 72 hrs was observed. Increased proliferation up to 10 nM with ˜60% proliferation. However, at lower concentrations no significant cell proliferation was observed (0.01 nM to 0.0001 nM) ( FIG.  2 AG ). 
     The proliferation effect of Diquas was assessed using human primary corneal epithelial cells. Cells were incubated with Diquas for 48 hrs and 72 hrs. Dose dependent increase in cell proliferation when the peptide was incubated with cells for 48 hrs or 72 hrs was observed. There is no significant proliferation observed from InM to 0.0001 nM ( FIG.  2 AH ). 
     The proliferation effect of YDE-012 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-012 for 48 hrs and 72 hrs. All tested concentration no significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs or 72 hrs ( FIG.  2 AI ). 
     The proliferation effect of YDE-019 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-019 for 48 hrs and 72 hrs. No significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations (0.1, 0.03 &amp; 0.01 μM) an increase in cell proliferation (˜20%) in 72 hrs incubation was observed ( FIG.  2 AJ ). 
     The proliferation effect of YDE-055 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-055 for 48 hrs and 72 hrs. No significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations (0.03 &amp; 0.01 μM) an increase in cell proliferation (25-30%) in 72 hrs incubation was observed ( FIG.  2 AK ). 
     The proliferation effect of YDE-076 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-076 for 48 hrs and 72 hrs. At concentrations 30, 10, μM, significant proliferation of cells was observed (&gt;20% over basal). This effect was observed when the cells were incubated for 48 hrs or 72 hrs. Other tested concentration no significant proliferation observed ( FIG.  2 AL ). 
     The proliferation effect of YDE-077 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-077 for 48 hrs and 72 hrs. No significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs. At 72 hrs incubation an increasing proliferation from 10 μM to 0.01 μM was observed ( FIG.  2 AM ). 
     The proliferation effect of YDE-078 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-078 for 48 hrs and 72 hrs. No significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs. At 72 hrs incubation an increasing proliferation from 3 μM to 0.01 μM was observed ( FIG.  2 AN ). 
     The proliferation effect of YDE-079 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-079 for 48 hrs and 72 hrs. No significant proliferation of cells (&lt;20% over basal) at higher concentration was observed (30-0.3 μM). This effect was observed when the cells were incubated for 48 hrs. At 72 hrs incubation an increasing significant proliferation from 30 μM to 0.01 μM (˜35% cell proliferation) was observed ( FIG.  2 AO ). 
     The proliferation effect of YDE-080 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-080 for 48 hrs and 72 hrs. At concentrations 30, 10 &amp;3 μM, dependent proliferation of cells was observed (&gt;20% over basal). This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentrations (1.0 to 0.01 μM) no significant cell proliferation in 48 hrs as well as 72 hrs incubation was observed ( FIG.  2 AP ). 
     The proliferation effect of YDE-081 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-081 for 48 hrs and 72 hrs. All tested concentrations no significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs or 72 hrs ( FIG.  2 AQ ). 
     The proliferation effect of YDE-082 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-082 for 48 hrs and 72 hrs. All tested concentrations, no significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs. At 72 hrs incubation an increasing proliferation from 1 μM to 0.01 μM was observed ( FIG.  2 AR ). 
     The proliferation effect of YDE-083 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-083 for 48 hrs and 72 hrs. All tested concentrations no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs. At 72 hrs incubation an increasing proliferation from 1 μM to 0.01 μM was observed ( FIG.  2 AS ). 
     The proliferation effect of YDE-084 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-084 for 48 hrs and 72 hrs. All tested concentrations, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs. At 72 hrs incubation an increasing proliferation from 10 μM to 0.01 μM was observed ( FIG.  2 AT ). 
     The proliferation effect of YDE-085 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-085 for 48 hrs and 72 hrs. Tested concentrations 30 μM to 0.1 μM, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs, whereas 0.03 &amp; 0.01 μM slightly increased proliferation. At 72 hrs incubation an increasing proliferation from 1 μM to 0.01 μM was observed ( FIG.  2 AU ). 
     The proliferation effect of YDE-086 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-086 for 48 hrs and 72 hrs. All tested concentrations, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs. At 72 hrs incubation an increasing proliferation from 1 μM to 0.01 μM ( FIG.  2 AV ). 
     The proliferation effect of YDE-087 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-087 for 48 hrs and 72 hrs. All tested concentrations, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs. At 72 hrs incubation an increasing proliferation from 3 μM to 0.01 μM was observed ( FIG.  2 AW ). 
     The proliferation effect of YDE-047 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-047 for 48 hrs and 72 hrs. No significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs. At lower concentrations (3 to 0.01 μM). An increase in cell proliferation (25-30%) in 72 hrs incubation was observed. ( FIG.  2 AX ). 
     The proliferation effect of YDE-048 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-048 for 48 hrs and 72 hrs. All tested concentrations, no significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs. At 72 hrs incubation an increasing proliferation from 1 μM to 0.01 μM was observed ( FIG.  2 AY ). 
     The proliferation effect of YDE-050 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-050 for 48 hrs and 72 hrs. All tested concentrations, no significant proliferation of cells was observed (&lt;20% over basal). This effect was observed when the cells were incubated for 48 hrs or 72 hrs. However, at lower concentration 0.01 μM, an increase in cell proliferation (30%) in 72 hrs incubation was observed ( FIG.  2 AZ ). 
     The proliferation effect of YDE-051 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-051 for 48 hrs and 72 hrs. All tested concentrations, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs or 72 hrs ( FIG.  2 BA ). 
     The proliferation effect of YDE-052 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-052 for 48 hrs and 72 hrs. All tested concentrations, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs or 72 hrs ( FIG.  2 BB ). 
     The proliferation effect of YDE-056 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-056 for 48 hrs and 72 hrs. At concentrations 30 to 0.01, μM, significant proliferation of cells (&gt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs whereas at 72 hrs incubation no significant proliferation was observed ( FIG.  2 BC ). 
     The proliferation effect of YDE-061 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-061 for 48 hrs and 72 hrs. At concentrations 30 to 0.01, μM, significant proliferation of cells (&gt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs whereas at 72 hrs incubation no significant proliferation was observed ( FIG.  2 BD ). 
     The proliferation effect of YDE-062 was assessed using human primary corneal epithelial cells. Cells were incubated with YDE-062 for 48 hrs and 72 hrs. All tested concentrations, no significant proliferation of cells (&lt;20% over basal) was observed. This effect was observed when the cells were incubated for 48 hrs where as 72 hrs showed slightly increase in proliferation ( FIG.  2 BE ). 
     Human epidermal growth factor (hEGF) as used as reference compound to assess the cell proliferation assays. Concentration dependent proliferation of primary corneal epithelial cells was observed. A maximum of ˜40% proliferation at highest tested concentration of 30 μM at 48 hrs incubation was observed ( FIG.  2 BF ). 
     Example 4: Assessment of the Effect of YDE053, YDE060, or YDE065 on Soluble Proteins Release in Poly I:C Stimulated Primary Human Corneal Epithelial Cells 
     Study Objective 
     To determine the effect of compound by assessing the panel of soluble proteins release in poly I:C stimulated primary human corneal epithelial cells by Multiplex. 
     Study Plan 
     
         
         
           
             Test System: Primary Human Corneal Epithelial cells 
             Assay Format: 96-well plate format 
             Method of Detection: Enzyme-linked immunosorbent assay 
             Assay Controls: Hinokitiol 
           
         
       
    
     Experimental Design 
     Assay protocol as described below was used to determine the significant effect of test compounds. The data was statistically analyzed by one-way ANOVA with a Bonferroni post test comparing all the columns. The significance was represented by the p value. 

 
     Results &amp; Conclusion 
     Results are shown in  FIG.  3   . IL-1beta, IL-6, IL-8, MIP-1alpha, MIP-1beta, RANTES, and TNF-alpha cytokine release was observed in human corneal epithelial cells. Stimulation with Poly I:C resulted in a significantly increase of IL-1beta, IL-6, IL-8, MIP-1alpha, MIP-1beta, RANTES, and TNF-alpha cytokine levels. 
     Reference compound, Hinokitiol, was found to significantly attenuate the Poly I:C stimulated cytokines release (IL-1beta, IL-6, IL-8, MIP-1alpha, MIP-1beta, RANTES, and TNF-alpha) in a dose dependent manner. 
     Test compounds YDE053, YDE060 and YDE065 were found to attenuate Poly I:C induced IL-6 cytokine release in a dose dependent manner. This was corroborated by the significant attenuation of IL-6 cytokine release at the tested concentrations that included 30 μM, 10 μM, and 1 μM respectively. 
     Effect of YDE053 on IL-6: Stimulated (MFI Mean±SD: 14801±407) vs Stimulated+30 μM Hinokitiol (MFI Mean±SD: 6759±500); p&lt;0.05, Stimulated vs Stimulated+10 μM Hinokitiol (MFI Mean±SD: 7011±471) p&lt;0.05, and Stimulated vs. Stimulated+1 μM Hinokitiol (MFI Mean±SD: 7004±376) p&lt;0.05. 
     Example 5: Determine the Effect of YDE053 Peptide on NF-kB (p65) Transcription Activity in Poly I:C Stimulated Primary Human Corneal Epithelial Cells 
     Study Objective 
     To determine the effect of YDE053 on NF-kB (p65) transcription activity in poly I:C stimulated primary human corneal epithelial cells. 
     Study Plan
         Test System: Primary Human Corneal Epithelial cells   Assay Format: 96-well plate format   Method of Detection: Enzyme-linked immunosorbent assay   Assay Controls: Hinokitiol       

     Experimental Design 
     Assay protocol as shown below was used to determine the significant effect of test compounds. The data was statistically analyzed by one-way ANOVA with a Bonferroni post test comparing all the columns. The significance was represented by the p value. 

 
     Results &amp; Conclusion 
     Results are shown in  FIG.  4   . Poly I:C stimulation on primary human corneal epithelial cells was found to result in the phosphorylation of (p65) NF-kB (Unstimulated cells: OD Mean±SD: 0.047±0.002 vs Stimulated: OD Mean±SD: 0.121±0.01; p&lt;0.05). 
     Reference compound, Hinokitiol was found to significantly attenuate the phosphorylation of (p65) NF-kB in Poly I:C stimulated cells in a dose dependent manner. The effect of YDE053 was found to significantly attenuate the phosphorylation of (p65) NF-kB in Poly I: C induced cells. This was corroborated by stimulated cells (OD Mean±SD: 0.121±0.01) vs cells treated with 30 μM YDE053 (OD Mean±SD: 0.049±0.002); p&lt;0.05, Stimulated cells (OD Mean±SD: 0.121±0.01) vs Cells treated with 10 μM YDE053 (OD Mean±SD: 0.06±0.006) p&lt;0.05. Test compound YDE053 reduced (p65) NF-kB transcription activity in a dose dependent manner at the tested concentrations. 
     Example 6: Determine the Effect of Compounds on Soluble IL6 Cytokine Release in Poly I:C Stimulated Primary Human Corneal Epithelial Cells 
     Study Objective 
     To determine the effect of compound by assessing the soluble IL-6 cytokine release in poly I:C stimulated primary human corneal epithelial cells by ELISA. 
     Study Plan 
     
         
         
           
             Test System: Primary Human Corneal Epithelial cells 
             Assay Format: 96-well plate format 
             Method of Detection: Enzyme-linked immunosorbent assay 
             Assay Controls: Hinokitiol 
           
         
       
    
     Experimental Design 
     Assay protocol as shown below was used to determine the significant effect of test compounds. The data was statistically analyzed by one-way ANOVA with a Bonferroni post test comparing all the columns. The significance was represented by the p value. 

 
     Results &amp; Conclusion 
     Results are shown in  FIG.  5   . Human corneal epithelial cells on stimulation with Poly I:C resulted in a significantly increase of IL-6 cytokine levels. Reference compound, Hinokitiol was found to significantly attenuate the Poly I:C stimulated IL-6 cytokine release in a dose dependent manner. Test compounds; YDE053, YDE048, YDE056, YDE057, YDE058, YDE067, YDE079, YDE011, YDE093, YDE096, YDE043 were found to attenuate Poly I:C induced of IL-6 cytokine release in a dose dependent manner. This was corroborated by the significant attenuation of IL-6 cytokine release at the tested concentrations that included; 30 μM, 10 μM, 1 μM and 0.1 μM respectively. 
     Example 7: In Vitro Evaluation of Test Compounds for Apparent Permeability Using 21 Day Cultured Caco-2 Cell Monolayer 
     Study Design &amp; Materials &amp; Methods 
       
     
       
         
           
               
               
             
               
                 TABLE 2 
               
               
                   
               
             
            
               
                 Cell origin 
                 American Type Culture Collection 
               
               
                   
                 (ATCC) 
               
               
                 Test System 
                 Caco-2 cells monolayer in 96 well format 
               
               
                 Passage number 
                 36 
               
               
                 Test plate 
                 Poly carbonate high pore density from 
               
               
                   
                 Millipore with 0.4 μm pore size, 0.11 
               
               
                   
                 cm 2  active membrane area 
               
               
                 Test compound concentration 
                 2 μM 
               
               
                 Bidirectional assay (A-B &amp; 
                 0 and 120 minutes 
               
               
                 B-A) time points 
               
               
                 Buffer in Receiver wells 
                 HBSS Buffer 
               
               
                 Replicates 
                 Two 
               
               
                 End Point 
                 Apparent permeability (Papp) 
               
               
                 Bio analysis 
                 LC-MS/MS 
               
               
                 QC Compounds 
                 Propranolol (High Permeable) and 
               
               
                   
                 Atenolol (Low Permeable) 
               
               
                 Assay Type 
                 Bi-directional (A-B and B-A) 
               
               
                 Cell Density 
                 12,000 cells/well (96 well plate) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 3 
               
               
                   
                   
               
               
                   
                 Test 
                   
                   
               
               
                   
                 Article 
                 Compound 
                 Molecular weight 
               
               
                   
                   
               
             
            
               
                   
                 1 
                 YDE-011 
                 953.10 
               
               
                   
                 2 
                 YDE-043 
                 486.50 
               
               
                   
                   
               
            
           
         
       
     
     Reagent Preparation 
     Preparation of (Dulbecco&#39;s Modified Eagles Medium) DMEM medium pH 7.4: 
     5 mL of 100 mM Sodium pyruvate, 5 mL of 100× non-essential amino acids, 5 mL of Penstrep was added to 100 mL of heat inactivated fetal bovine serum to 385 mL of DMEM aseptically and mixed thoroughly. 
     Preparation of Hank&#39;s Balanced Salt Solution (HBSS) pH 7.4: 
     One vial of Hank&#39;s balanced salt (Sigma-H1387) was dissolved in 900 mL of milli Q water; pH was adjusted to 7.4 and made up the volume to 1000 mL of water. The solution was filter sterilized and store at 4° C. 
     Preparation &amp; Dilution of Test Compound: 
     10 mM stock solution of test compound was prepared in DMSO. 10 mM stock was diluted with 100% DMSO to prepare 0.2 mM, 0.2 mM stock was diluted with HBSS buffer to a final concentration of 2 μM. 
     Assay Procedure 
     Seeding of the Cells: 
     Add 250 μL of DMEM to the basal compartment of 96 well multi-screen Caco-2 plates and seed 12000 cells/well (0.16×10 6  cells/ml) in all the apical wells required and 2 wells with only media as blank without cells. Place the Caco-2 plate in CO 2  incubator at 37° C. for proliferation of cells. 
     Media Change: 
     The utilized media was replenished every alternate day by fresh medium. On 21st day, utilized medium was removed and washed twice with HBSS Buffer and incubated with HBSS buffer 30 min in incubator and initiated the assay. 
     Apical to Basal Permeability: 
     75 μL of test compound was added to apical wells and 250 μL of HBSS buffer with 1% DMSO was added to basal wells. Samples were collected at 120 min and processed as stated below. 
     Basal to Apical Permeability: 
     250 μL of test compound was added to basal wells and 75 μL of HBSS buffer with 1% DMSO was added to apical wells. Samples were collected at 120 min and processed as stated below. 
     Sampling Processing: 
     Single point calibration curve in HBSS buffer was used. 100 μL of supernatant was diluted with 200 μL of water and submitted for LC-MS/MS analysis. 
     Calculations: 
       Papp= dQ/dT× 1/ Co× 1/ A,    
     dQ is amount collected in the basolateral compartment of the 96 well filter plate; 
     dT is Time of incubation of drug on the cell monolayer; 
     Co is initial concentration of drug in the apical compartment of the well; 
     A is surface area of the filter. 
       Efflux ratio=Papp of basal to apical samples/Papp of apical to basal samples 
       Recovery: {( dQ  of Apical+ dQ  of Basal)/Standard  dQ}* 100 
     Results 
     The Caco-2 Permeability results of the test compounds is represented in below Table 4. 
     
       
         
           
               
               
             
               
                   
                 TABLE 4 
               
             
            
               
                   
                   
               
               
                   
                 Average Values 
               
            
           
           
               
               
               
            
               
                   
                 Papp (10 −6  cm/sec) 
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Compound 
                 Apical to 
                 Basal to 
                 Efflux 
                 A to B % 
                 B to A % 
                   
               
               
                 Name 
                 Basal 
                 Apical 
                 Ratio 
                 Recovery 
                 Recovery 
                 Classification 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 YDE-011 
                 0.00 
                 0.00 
                 NC 
                 15.00 
                 88.50 
                 LOW 
               
               
                 YDE-043 
                 0.00 
                 0.00 
                 NC 
                 34.00 
                 67.25 
                 LOW 
               
               
                 Propranolol 
                 23.83 
                 37.07 
                 1.56 
                 80.07 
                 90.30 
                 HIGH 
               
               
                 Atenolol 
                 0.00 
                 0.57 
                 NC 
                 93.90 
                 85.33 
                 LOW 
               
               
                   
               
               
                 NC: denotes Not calculated 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Data Interpretation: Criteria for classification 
               
               
                 of the compounds (Caco-2 Permeability). 
               
            
           
           
               
               
            
               
                 Result of permeability Papp 
                   
               
               
                 (10 −6  cm/s) 
                 Range 
               
               
                   
               
               
                  &lt;1 
                 Low permeable 
               
               
                 1-10 
                 Medium permeable 
               
               
                 &gt;10 
                 High permeable 
               
               
                   
               
            
           
         
       
     
     Example 8: Investigate the Effects of YY-101, YDE-011 &amp; YDE-043 Compounds on Various Cytokine and Chemokine Release in Human Peripheral Blood Mononuclear Cells (PBMCs) (Study 1) 
     Background and Purpose 
     The objective of this study was to investigate the effects of YY-101, YDE-011 and YDE-043 compounds on various cytokine and chemokine release in human peripheral blood mononuclear cells (hPBMCs) stimulated by known stimulants such as LPS, poly I:C or PMA/Ionomycin. 
     To achieve this goal, a pilot study was conducted to establish a dose and time-dependent IL-6 cytokine release in hPBMCs by each stimulant. Upon selection of the dose and time point for the best stimulant, the main study was conducted to establish the efficacious dose-response curve of YY-101, YDE-011, YDE-043 on up to 30 cytokine and chemokine release in hPBMC induced by LPS. 
     Materials &amp; Methods 
     Reagents and Solutions 
     
         
         
           
             Human PBMCs (ATCC, USA) 
             Lipopolysaccharides from  Escherichia coli  (Sigma Aldrich, USA) 
             poly (I:C) (Sigma Aldrich, USA) 
             PMA (Sigma Aldrich, USA) 
             Ionomycin (Sigma Aldrich, USA) 
             Human IL-6 ELISA kit (Thermo fisher, USA) 
             Human magnetic Luminex assay kit (R&amp;D System, USA) 
             Xiidra® (Shire, USA) 
             Test materials (YY-101, YDE-011, YDE-043) were stored in a deep freezer (−20° C.). 
           
         
       
    
     Cell Culture 
     Human PBMCs frozen in a cryopreserve was thawed, washed with Hank&#39;s Balanced Salt Solution containing 10% Fetal bovine serum, and seeded onto a 24-well plate at a density of 1×10 6  cells/well with culture RPMI media containing 10% Fetal bovine serum in growth media in a 24 well plate for 12, 24, and 48 hrs and maintained at 37° C. in an atmosphere of 95% air and 5% CO 2 . 
     1 st  Pilot Study 
     Human PBMCs was pretreated with a reference compound; Xiidra® (the final concentration @ 10 uM) for 2 h before stimulation. An equal volume of 0.5% DMSO was used as a vehicle control. Then, the cells were treated with stimulant(s), poly(I:C) at 25 ug/ml, LPS at 5 and 25 ug/ml at and PMA (5 ng/ml)/Ionomycin (1 ug/ml). Triplicates of 100 ul of supernatants from treated cells was harvested at 12, 24, and 48 h and placed in 96 well for Human IL-6 ELISA measurement according to the manufacturer&#39;s instructions (Invitrogen, CA, USA). 
     The pilot study arm with triplicate wells per group in a 24-well plate:
         1. PBMC: 0.5% DMSO   2. PBMC+Poly I:C (25 ug/ml)   3. PBMC+LPS (5 ug/ml)   4. PBMC+LPS (25 ug/ml)   5. PBMC+PMA (5 ng/ml)/Ionomycin (1 ug/ml)   6. PBMC+Poly I:C (25 ug/ml)+Xiidra® 10 uM   7. PBMC+LPS (5 ug/ml)+Xiidra® 10 uM   8. PBMC+LPS (25 ug/ml)+Xiidra® 10 uM   9. PBMC+PMA (5 ng/ml)/Ionomycin (1 ug/ml)+Xiidra® 10 uM       

     2 nd  Pilot Study 
     Human PBMCs was pretreated with a test compound; YDE-011 at 30 uM, 1 μM for 2 h before stimulation. An equal volume of 0.5% DMSO was used as a vehicle control. Then, the cells were treated with stimulants, poly(I:C) at 25 ug/ml, LPS at 5 ug/ml at and PMA (5 ng/ml)/Ionomycin (1 ug/ml). The supernatants from treated cells were harvested at 24 h and placed in 96 well for Human IL-6 ELISAS measurement according to the manufacturer&#39;s instructions (Invitrogen, CA, USA). 
     The pilot study arm with duplicate wells per group in a 24-well plate:
         1. PBMC: 0.5% DMSO   2. PBMC+Poly I:C (25 ug/ml)   3. PBMC+LPS (5 ug/ml)   4. PBMC+PMA (5 ng/ml)/Ionomycin (1 ug/ml)   5. PBMC+Poly I:C (25 ug/ml)+Xiidra® 30 uM   6. PBMC+LPS (5 ug/ml)+Xiidra® 30 uM   7. PBMC+PMA (5 ng/ml)/Ionomycin (1 ug/ml)+Xiidra® 30 Um   8. PBMC+Poly I:C (25 ug/ml)+Xiidra® 1 uM   9. PBMC+LPS (5 ug/ml)+Xiidra® 1 uM   10. PBMC+PMA (5 ng/ml)/Ionomycin (1 ug/ml)+Xiidra® 1 uM       

     Human IL-6 ELISA 
     IL-6 measurement was performed by the sandwich ELISA method. The capture antibody was coated on a 96-well plate (Corning™ Costar™ 9018, NY, USA) at 100 ul/well and incubated overnight at 4° C. The interaction was blocked at room temperature for 2 h to prevent non-specific binding of the antigen-antibody. Then, the plate was incubated overnight at 4° C. with 1:200 diluted cell supernatants and standard dilute serial dilutions at 100 ul/well. Detection antibody was dispensed into the plate at 100 ul/well, and then allowed to incubate at a room temperature for 1 h. Finally, streptavidin-HRP (100 ul/well) was incubated at room temperature for 30 min. TMB substrate (100 ul/well) was added and allowed to incubate for 15 min until colored reaction was observed in the dark condition. The sample reading was measured at 450 nm wavelength and 570 nm, and the quantification of the sample was converted as concentration (ng/ml) based on the standard curve extrapolation. 
     The Main Study 
     Human PBMCs were pretreated with YY-101, YDE-011, YDE-043 or reference compound; Xiidra® at various concentration (ranging from 5 μM to 500 nM) for 2 h before stimulation. An equal volume of 0.5% DMSO was used as a vehicle control. Then, the cells were treated with LPS at 5 ug/ml, which was selected based on the pilot study. The supernatants from treated cells were harvested at 24 h and placed in a 96 well for Luminex cytokine &amp; chemokine profiling using a Luminex 200 multiplex assay (Luminex; R&amp;D system, USA). 
     The main study arm with triplicate wells per group in a 24-well plate:
         1. Blank   2. PBMC: 0.5% DMSO   3. PBMC+LPS (5 ug/ml)   4. PBMC+YY-101 500 nM   5. PBMC+YY-101 50 nM   6. PBMC+YY-101 5 nM   7. PBMC+YY-101 0.5 nM   8. PBMC+LPS (5 ug/ml)+Xiidra® 500 nM   9. PBMC+LPS (5 ug/ml)+Xiidra® 50 nM   10. PBMC+LPS (5 ug/ml)+Xiidra® 5 nM   11. PBMC+LPS (5 ug/ml)+Xiidra® 0.5 nM   12. PBMC+LPS (5 ug/ml)+Xiidra® 0.05 nM   13. PBMC+LPS (5 ug/ml)+Xiidra® 0.005 nM   14. PBMC+LPS (5 ug/ml)+YY-101 500 nM   15. PBMC+LPS (5 ug/ml)+YY-101 50 nM   16. PBMC+LPS (5 ug/ml)+YY-101 5 nM   17. PBMC+LPS (5 ug/ml)+YY-101 0.5 nM   18. PBMC+LPS (5 ug/ml)+YY-101 0.05 nM   19. PBMC+LPS (5 ug/ml)+YY-101 0.005 nM   20. PBMC+LPS (5 ug/ml)+YDE-011 500 nM   21. PBMC+LPS (5 ug/ml)+YDE-011 50 nM   22. PBMC+LPS (5 ug/ml)+YDE-011 5 nM   23. PBMC+LPS (5 ug/ml)+YDE-011 0.5 nM   24. PBMC+LPS (5 ug/ml)+YDE-011 0.05 nM   25. PBMC+LPS (5 ug/ml)+YDE-011 0.005 nM   26. PBMC+LPS (5 ug/ml)+YDE-043 500 nM   27. PBMC+LPS (5 ug/ml)+YDE-043 50 nM   28. PBMC+LPS (5 ug/ml)+YDE-043 5 nM   29. PBMC+LPS (5 ug/ml)+YDE-043 0.5 nM   30. PBMC+LPS (5 ug/ml)+YDE-043 0.05 nM   31. PBMC+LPS (5 ug/ml)+YDE-043 0.005 nM       

     Multiplex Assay 
     Multiplex assay was used to measure 30 cytokines and chemokines. The 1:200 diluted cell supernatant and serial dilution of the standard were dispensed into a 96 well plate at 50 ul/well. Then, the pre-mixed cocktail of antibody-coated magnetic beads was dispensed at 50 ul/well and incubated at room temperature for 2 h in a horizontal orbital microplate shaker at 800±500 rpm. The beads were washed using a magnetic device to prevent loss. The biotin-antibody was dispensed in 50 ul of each well and incubated at room temperature for 1 h in a shaker at 800±500 rpm. After washing, streptavidin-PE was added at 50 ul/well and incubated at room temperature for 30 min in a shaker under the same conditions. Finally, after washing, wash buffer (100 ul/well) was added to the plate and incubated in a shaker for 2 min. The Luminex™ 200 setting were set according to the manufacturer&#39;s protocol. The data was calculated with a standard five-parameter logistic nonlinear regression analysis of the data (xPonent software 4.2, USA). Results were presented as concentration (ng/ml or pg/ml). 
     Below is the list of cytokines and chemokines either in 26-plex or 4-plex. 
     
       
         
           
               
               
             
               
                 TABLE 6 
               
               
                   
               
               
                   
                 Cytokine/Chemokine 
               
               
                 Human 
                 list 
               
               
                   
               
             
            
               
                 26 plex 
                   
               
               
                 1 
                 MCP-1 (CCL2) 
               
               
                 2 
                 MIP-1 alpha (CCL3) 
               
               
                 3 
                 MIP-3 alpha (CCL20) 
               
               
                 4 
                 Fractalkine (CX3CL1) 
               
               
                 5 
                 Fas-L 
               
               
                 6 
                 GM-CSF 
               
               
                 7 
                 ICAM-1 
               
               
                 8 
                 IFN-gamma 
               
               
                 9 
                 IL-1 alpha 
               
               
                 10 
                 IL-1 beta 
               
               
                 11 
                 IL-2 
               
               
                 12 
                 IL-4 
               
               
                 13 
                 IL-5 
               
               
                 14 
                 IL-6 
               
               
                 15 
                 IL-8 (CXCL8) 
               
               
                 16 
                 IL-10 
               
               
                 17 
                 IL-13 
               
               
                 18 
                 IL-17A 
               
               
                 19 
                 Leptin 
               
               
                 20 
                 MMP-3 
               
               
                 21 
                 MMP-8 
               
               
                 22 
                 MMP-9 
               
               
                 23 
                 RAGE 
               
               
                 24 
                 L-Selectin 
               
               
                 25 
                 TNF-alpha 
               
               
                 26 
                 VEGF-D 
               
               
                 4 plex 
               
               
                 1 
                 MIP-1 beta (CCL4) 
               
               
                 2 
                 RANTES (CCL5) 
               
               
                 3 
                 TIMP-1 
               
               
                 4 
                 VEGF-A 
               
               
                   
               
            
           
         
       
     
     Compound Delivery &amp; Formulation 
     The concentration of compound was prepared according to the information provided by the client. 
     All compound was serially diluted in from the stock concentrations and the final concentration of DMSO was not exceed 0.5% DMSO. All compounds were prepared on the same day of the treatment. 
     Statistical Analysis 
     All values are presented as mean±standard error of mean (SEM). The statistical significance of the results was analyzed using one-way ANOVA with a Bonferroni post hoc. The statistical analyses were performed using the SPSS software (SPSS 22.0, USA). Each compound treated PBMCs was compared to that of the stimulant induced PBMCs. The significant threshold was fixed at 0.05 i.e. p value has to be lower than 0.05 to be significant. 
     Results 
     Results are shown in  FIGS.  6 - 22   . Human PBMCs frozen purchased from ATCC in a cryopreservative were thawed, washed with Hank&#39;s Balanced Salt Solution containing 10% fetal bovine serum, and seeded onto a 24-well plate at a density of 1×106 cells/well with culture RPMI media containing 10% fetal bovine serum in growth media in a 24 well plate for 24 hrs and maintained at 37° C. in an atmosphere of 95% air and 5% CO2. To establish, a pilot study was conducted to establish the timeline and pro-inflammatory stimulant, the cells are treated with stimulant(s), poly(I:C) at 25 ug/mL, LPS at 5 or 25 ug/mL at or PMA (5 ng/ml)/ionomycin (1 ug/ml), supernatants from treated cells are harvested at 12, 24 &amp; 48 h and placed in 96 well for IL-6 ELISA measurement. For the main study, at the end of 24 hr culture, cells are pretreated with YY-101, YDE-011, YDE-043 or reference compound, Xiidra® at various concentrations for 2 h before stimulation. An equal volume of 0.5% DMSO is used as a vehicle control. Then, the cells are treated with LPS. 20 uL of supernatants from treated cells are harvested at 24 hr post-LPS treatment and placed in 96 well for Luminex cytokine &amp; chemokine profiling using a Luminex 200 multiplex assay. 
     In the pilot studies, all three stimulants (poly I:C, LPS and PMA/Ionomycin) significantly induced IL-6 production in hPBMCs in a time-dependent manner. The reference compound, Xiidra® and YDE-011 significantly reduced IL-6 levels induced by LPS or poly I:C. In the main test, hPBMCs stimulated by LPS at 5 ug/mL for 24 hrs were co-treated with test and reference compounds with various concentrations and from which cytokine release was measured. Among 30 cytokines and chemokines evaluated, YY-101, YDE-011 and YDE-043 at as low as 5 μM significantly reduced LPS-induced pro-inflammatory cytokines and chemokines, IL-2, IL-6, IL-8, IL-10, TNF-α, MMP3, CCL-2, CCL-3 and CCL-4. Among the compounds, YDE-043 significantly lowered LPS-induced IL-1a and IL-1b levels. In contrast, Xiidra® up to 500 nM didn&#39;t affect any of LPS-induced cytokine/chemokine levels. Interestingly, the compound YY-101 didn&#39;t affect the basal levels of cytokine production. 
     IL-6 ELISA in 1 st  Pilot Test: 
     IL-6 was measured by sandwich ELISA assay. poly I:C, LPS and PMA/Ionomycin significantly induced IL-6 production in hPBMCs in a time-dependent manner. Among all, LPS induced the highest level of IL-6 at 5 and 25 ug/mL equally. 
     In the presence of Xiidra® at 30 μM, the reference compound, IL-6 induction was moderately reduced in all stimulants at 24 and 48 hr. Based on strong IL-6 stimulation induced by LPS and poly I:C at 24 and 48 hrs and moderate effects of Xiidra® in the first pilot study, the second pilot study was designed to repeat the stimulants&#39; effect and to elucidate the compound, YDE-011&#39;s modulatory effects. 
     IL-6 ELISA in 2 nd  Pilot Test. 
     Similar to the first pilot study, a similar level of IL-6 induction was observed when hPBMCs were stimulated by poly I:C, LPS at 5 ug/mL or PMA/Ionomycin. YDE-011 at 1 uM effectively and significantly reduced IL-6 induction in PBMCs stimulated by poly I:C, LPS or PMA/Ionomycin. The positive modulatory effect of YDE-011 was diminished at a higher concentration. 
     Multi-Cytokine Assessment in PBMCs Stimulated by LPS. 
     In the main test, hPBMCs were stimulated by LPS at 5 ug/mL for 24 hrs and co-treated with vehicle (0.5% DMSO), test articles and reference compound, Xiidra® at various concentrations (500 nM to 5 μM). After 24 hr treatment, aliquots of hPBMC media were extracted, diluted in 1:200, and measured for cytokine levels using Luminex multiplex system. 
     Among 30 cytokines and chemokines evaluated, levels of 14 cytokines/chemokines passed the detection QC and the levels of 16 cytokines below the detection levels. 
     Conclusions 
     LPS effectively induced various pro-inflammatory cytokines in human PBMCs and its induction was potently and significantly reduced by all the compounds. Among those compounds, YDE-043 more effectively lowered cytokine and chemokine production. The reference marketed compound, Xiidra® at similar concentrations didn&#39;t modulate any of cytokine/chemokine levels, suggesting that YY and YDE compounds are more potent and effective immunomodulators in lowering pro-inflammatory cytokine/chemokine productions in human PBMCs when stimulated by endotoxins, such as LPS. 
     In conclusion, all of the test compounds, YY-101, YDE-011, YDE-043, potently and significantly reduced pro-inflammatory cytokine/chemokine production in human PBMCs stimulated by LPS. 
     Annex 1: 1 st  Pilot Study Raw Data 
       
     
       
         
           
               
               
               
               
             
               
                 TABLE 7 
               
               
                   
               
               
                 Sample 
                 12 hr 
                 24 hr 
                 48 hr 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 PBMC + 0.5% DMSO 
                 1st 
                 0 
                 0 
                 0 
               
               
                   
                 2nd 
                 0 
                 0 
                 0 
               
               
                   
                 3rd 
                 0 
                 0 
                 0 
               
               
                   
                 Mean 
                 0 
                 0 
                 0 
               
               
                   
                 STDER 
                 0 
                 0 
                 0 
               
               
                 PBMC + Poly I:C(25 ug/ml) 
                 1st 
                 15338.9 
                 20950.9 
                 26257.3 
               
               
                   
                 2nd 
                 11022.2 
                 17695.4 
                 21275.3 
               
               
                   
                 3rd 
                 11068.1 
                 18514.6 
                 19606 
               
               
                   
                 Mean 
                 12476.4 
                 19053.6 
                 22379.5 
               
               
                   
                 STDER 
                 1431.3 
                 977.7 
                 1997.9 
               
               
                 PBMC + Poly I:C(25 ug/ml) + 
                 1st 
                 16701.1 
                 14320.1 
                 18990.1 
               
               
                 Xiidra ® 10 uM 
                 2nd 
                 15193.1 
                 15773 
                 19515.5 
               
               
                   
                 3rd 
                 14460 
                 16445.9 
                 23645 
               
               
                   
                 Mean 
                 15451.4 
                 15513 
                 20716.8 
               
               
                   
                 STDER 
                 659.7 
                 627.3 
                 1471.9 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 14335.4 
                 23909.6 
                 33897.3 
               
               
                   
                 2nd 
                 18694.5 
                 22492.3 
                 31019.9 
               
               
                   
                 3rd 
                 17440.3 
                 21943.3 
                 30259.1 
               
               
                   
                 Mean 
                 16823.4 
                 22781.7 
                 31725.4 
               
               
                   
                 STDER 
                 1295.6 
                 585.8 
                 1107.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 14546.1 
                 17436.8 
                 23737.8 
               
               
                 Xiidra ® 10 uM 
                 2nd 
                 14465.7 
                 18053 
                 24780.7 
               
               
                   
                 3rd 
                 13637.7 
                 17850.2 
                 27375.7 
               
               
                   
                 Mean 
                 14216.5 
                 17780 
                 25298.1 
               
               
                   
                 STDER 
                 290.3 
                 181.3 
                 1081.6 
               
               
                 PBMC + LPS(25 ug/ml) 
                 1st 
                 16538 
                 21652.8 
                 32026.4 
               
               
                   
                 2nd 
                 16547.8 
                 20628.4 
                 28839.7 
               
               
                   
                 3rd 
                 15060.5 
                 21084.2 
                 31748.5 
               
               
                   
                 Mean 
                 16048.8 
                 21121.8 
                 30871.5 
               
               
                   
                 STDER 
                 494.2 
                 296.3 
                 1019.1 
               
               
                 PBMC + LPS(25 ug/ml) + 
                 1st 
                 12065 
                 18166.2 
                 24395.9 
               
               
                 Xiidra ® 10 uM 
                 2nd 
                 20113.3 
                 16796.5 
                 24754.1 
               
               
                   
                 3rd 
                 17779.6 
                 17509.3 
                 29892.3 
               
               
                   
                 Mean 
                 16652.7 
                 17490.7 
                 26347.5 
               
               
                   
                 STDER 
                 2390.7 
                 395.5 
                 1775.5 
               
               
                 PBMC + PMA(5 
                 1st 
                 1265.6 
                 5302.9 
                 12535.4 
               
               
                 ng/ml)/Ionomycin(0.5 ug/ml) 
                 2nd 
                 1186.5 
                 4731.7 
                 12151.6 
               
               
                   
                 3rd 
                 1500.8 
                 4977.8 
                 13166.8 
               
               
                   
                 Mean 
                 1317.6 
                 5004.1 
                 12617.9 
               
               
                   
                 STDER 
                 94.4 
                 165.4 
                 296 
               
               
                 PBMC + PMA(5 
                 1st 
                 1150.9 
                 3528 
                 7815.5 
               
               
                 ng/ml)/Ionomycin(0.5 ug/ml) + 
                 2nd 
                 1196.5 
                 3455.6 
                 8506.2 
               
               
                 Xiidra ® 10 uM 
                 3rd 
                 1096.5 
                 3622.9 
                 9128.3 
               
               
                   
                 Mean 
                 1148 
                 3535.5 
                 8483.3 
               
               
                   
                 STDER 
                 28.9 
                 48.5 
                 379.1 
               
               
                   
               
            
           
         
       
     
     Annex 2: 2 nd  Pilot Study Raw Data 
       
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 8 
               
               
                   
                   
               
               
                   
                 Sample 
                 24 hr 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 PBMC + 0.5% DMSO 
                 1st 
                 0 
               
               
                   
                   
                 2nd 
                 0 
               
               
                   
                   
                 Mean 
                 0 
               
               
                   
                   
                 STDER 
                 0 
               
               
                   
                 PBMC + Poly I:C(25 ug/ml) 
                 1st 
                 24239.8 
               
               
                   
                   
                 2nd 
                 18619.3 
               
               
                   
                   
                 Mean 
                 21429.5 
               
               
                   
                   
                 STDER 
                 2810.2 
               
               
                   
                 PBMC + Poly I:C(25 ug/ml) + 
                 1st 
                 39230.7 
               
               
                   
                 YDE-011 30 uM 
                 2nd 
                 30634.7 
               
               
                   
                   
                 Mean 
                 34932.7 
               
               
                   
                   
                 STDER 
                 4298 
               
               
                   
                 PBMC + Poly I:C(25 ug/ml) + 
                 1st 
                 13701 
               
               
                   
                 YDE-011 1 uM 
                 2nd 
                 12585.1 
               
               
                   
                   
                 Mean 
                 13143.1 
               
               
                   
                   
                 STDER 
                 557.9 
               
               
                   
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 29610.6 
               
               
                   
                   
                 2nd 
                 22576 
               
               
                   
                   
                 Mean 
                 26093.3 
               
               
                   
                   
                 STDER 
                 3517.3 
               
               
                   
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 23199.5 
               
               
                   
                 YDE-011 30 uM 
                 2nd 
                 18310.8 
               
               
                   
                   
                 Mean 
                 20755.2 
               
               
                   
                   
                 STDER 
                 2444.3 
               
               
                   
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 14532.1 
               
               
                   
                 YDE-011 1 uM 
                 2nd 
                 12561.2 
               
               
                   
                   
                 Mean 
                 13546.7 
               
               
                   
                   
                 STDER 
                 985.4 
               
               
                   
                 PBMC + PMA(1 
                 1st 
                 3031.7 
               
               
                   
                 ng/ml)/Ionomycin(0.5 ug/ml) 
                 2nd 
                 5119.3 
               
               
                   
                   
                 Mean 
                 4075.5 
               
               
                   
                   
                 STDER 
                 1043.8 
               
               
                   
                 PBMC + PMA(1 
                 1st 
                 3114.6 
               
               
                   
                 ng/ml)/Ionomycin(0.5 ug/ml + 
                 2nd 
                 3025 
               
               
                   
                 YDE-011 30 uM 
                 Mean 
                 3069.8 
               
               
                   
                   
                 STDER 
                 44.8 
               
               
                   
                 PBMC + PMA(1 
                 1st 
                 2817.2 
               
               
                   
                 ng/ml)/Ionomycin(0.5 ug/ml + 
                 2nd 
                 3828.2 
               
               
                   
                 YDE-011 1 uM 
                 Mean 
                 3322.7 
               
               
                   
                   
                 STDER 
                 505.5 
               
               
                   
                   
               
            
           
         
       
     
     Annex 3. The Main Study Raw Data 
       
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Bead name: IL-2 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concentration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 Dilution 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 29.0 
                 67.0 
                 1.0 
                 &lt;0.45 
               
               
                   
                 2nd 
                 31.0 
                 67.0 
                 1.0 
                 0.45 
               
               
                   
                 Mean 
                 30.0 
                 67.0 
                 1.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 30.0 
                 87.0 
                 200.0 
                 5.9 
               
               
                   
                 2nd 
                 30.0 
                 86.0 
                 200.0 
                 5.9 
               
               
                   
                 3rd 
                 32.0 
                 107.0 
                 200.0 
                 173.5 
               
               
                   
                 Mean 
                 30.7 
                 93.3 
                 200.0 
                 61.8 
               
               
                   
                 STDER 
                 0.7 
                 6.8 
                 0.0 
                 55.9 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 88.5 
                 68.0 
                 200.0 
                 4607.6 
               
               
                   
                 2nd 
                 74.0 
                 85.0 
                 200.0 
                 3485.4 
               
               
                   
                 3rd 
                 79.0 
                 107.0 
                 200.0 
                 3873.0 
               
               
                   
                 Mean 
                 80.5 
                 86.7 
                 200.0 
                 3988.7 
               
               
                   
                 STDER 
                 4.3 
                 11.3 
                 0.0 
                 329.1 
               
               
                 PBMC + YY101 500 nM 
                 1st 
                 31.5 
                 86.0 
                 200.0 
                 132.3 
               
               
                   
                 2nd 
                 33.0 
                 102.0 
                 200.0 
                 255.5 
               
               
                   
                 Mean 
                 32.3 
                 94.0 
                 200.0 
                 193.9 
               
               
                   
                 STDER 
                 0.8 
                 8.0 
                 0.0 
                 61.6 
               
               
                 PBMC + YY101 50 nM 
                 1st 
                 30.5 
                 88.0 
                 200.0 
                 48.8 
               
               
                   
                 2nd 
                 32.0 
                 75.0 
                 200.0 
                 173.5 
               
               
                   
                 Mean 
                 31.3 
                 81.5 
                 200.0 
                 111.2 
               
               
                   
                 STDER 
                 0.8 
                 6.5 
                 0.0 
                 62.4 
               
               
                 PBMC + YY101 5 nM 
                 1st 
                 32.0 
                 86.0 
                 200.0 
                 173.5 
               
               
                   
                 2nd 
                 32.0 
                 101.0 
                 200.0 
                 173.5 
               
               
                   
                 Mean 
                 32.0 
                 93.5 
                 200.0 
                 173.5 
               
               
                   
                 STDER 
                 0.0 
                 7.5 
                 0.0 
                 0.0 
               
               
                 PBMC + YY101 0.5 nM 
                 1st 
                 32.0 
                 84.0 
                 200.0 
                 173.5 
               
               
                   
                 2nd 
                 34.0 
                 92.0 
                 200.0 
                 337.0 
               
               
                   
                 Mean 
                 33.0 
                 88.0 
                 200.0 
                 255.2 
               
               
                   
                 STDER 
                 1.0 
                 4.0 
                 0.0 
                 81.7 
               
               
                 PBMC + YY101 0.05 uM 
                 1st 
                 38.0 
                 105.0 
                 200.0 
                 659.4 
               
               
                   
                 2nd 
                 34.0 
                 92.0 
                 200.0 
                 337.0 
               
               
                   
                 Mean 
                 36.0 
                 98.5 
                 200.0 
                 498.2 
               
               
                   
                 STDER 
                 2.0 
                 6.5 
                 0.0 
                 161.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 88.0 
                 101.0 
                 200.0 
                 4569.0 
               
               
                 Xiidra 500 nM 
                 2nd 
                 86.0 
                 97.0 
                 200.0 
                 4414.5 
               
               
                   
                 Mean 
                 87.0 
                 99.0 
                 200.0 
                 4491.8 
               
               
                   
                 STDER 
                 1.0 
                 2.0 
                 0.0 
                 77.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 90.0 
                 69.0 
                 200.0 
                 4723.4 
               
               
                 Xiidra 50 nM 
                 2nd 
                 93.0 
                 95.0 
                 200.0 
                 4954.9 
               
               
                   
                 Mean 
                 91.5 
                 82.0 
                 200.0 
                 4839.1 
               
               
                   
                 STDER 
                 1.5 
                 13.0 
                 0.0 
                 115.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 83.0 
                 70.0 
                 200.0 
                 4182.6 
               
               
                 Xiidra 5 nM 
                 2nd 
                 95.0 
                 79.0 
                 200.0 
                 5109.1 
               
               
                   
                 Mean 
                 89.0 
                 74.5 
                 200.0 
                 4645.8 
               
               
                   
                 STDER 
                 6.0 
                 4.5 
                 0.0 
                 463.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 76.0 
                 87.0 
                 200.0 
                 3640.5 
               
               
                 Xiidra 0.5 nM 
                 2nd 
                 79.5 
                 80.0 
                 200.0 
                 3911.7 
               
               
                   
                 Mean 
                 77.8 
                 83.5 
                 200.0 
                 3776.1 
               
               
                   
                 STDER 
                 1.8 
                 3.5 
                 0.0 
                 135.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 74.0 
                 103.0 
                 200.0 
                 3485.4 
               
               
                 Xiidra 0.05 nM 
                 2nd 
                 81.5 
                 94.0 
                 200.0 
                 4066.6 
               
               
                   
                 Mean 
                 77.8 
                 98.5 
                 200.0 
                 3776.0 
               
               
                   
                 STDER 
                 3.8 
                 4.5 
                 0.0 
                 290.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 79.0 
                 74.0 
                 200.0 
                 3873.0 
               
               
                 Xiidra 0.005 nM 
                 2nd 
                 72.0 
                 67.0 
                 200.0 
                 3330.1 
               
               
                   
                 Mean 
                 75.5 
                 70.5 
                 200.0 
                 3601.6 
               
               
                   
                 STDER 
                 3.5 
                 3.5 
                 0.0 
                 271.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 64.0 
                 68.0 
                 200.0 
                 2707.7 
               
               
                 YY101 500 nM 
                 2nd 
                 66.0 
                 84.0 
                 200.0 
                 2863.5 
               
               
                   
                 3rd 
                 62.0 
                 84.0 
                 200.0 
                 2551.7 
               
               
                   
                 Mean 
                 64.0 
                 78.7 
                 200.0 
                 2707.6 
               
               
                   
                 STDER 
                 1.2 
                 5.3 
                 0.0 
                 90.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 61.0 
                 79.0 
                 200.0 
                 2473.6 
               
               
                 YY101 50 nM 
                 2nd 
                 64.0 
                 96.0 
                 200.0 
                 2707.7 
               
               
                   
                 3rd 
                 66.0 
                 99.0 
                 200.0 
                 2863.5 
               
               
                   
                 Mean 
                 63.7 
                 91.3 
                 200.0 
                 2681.6 
               
               
                   
                 STDER 
                 1.5 
                 6.2 
                 0.0 
                 113.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 61.0 
                 87.0 
                 200.0 
                 2473.6 
               
               
                 YY101 5 nM 
                 2nd 
                 63.0 
                 83.0 
                 200.0 
                 2629.7 
               
               
                   
                 3rd 
                 67.0 
                 67.0 
                 200.0 
                 2941.4 
               
               
                   
                 Mean 
                 63.7 
                 79.0 
                 200.0 
                 2681.6 
               
               
                   
                 STDER 
                 1.8 
                 6.1 
                 0.0 
                 137.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 66.0 
                 102.0 
                 200.0 
                 2863.5 
               
               
                 YY101 0.5 nM 
                 2nd 
                 65.0 
                 87.0 
                 200.0 
                 2785.6 
               
               
                   
                 3rd 
                 65.5 
                 74.0 
                 200.0 
                 2824.6 
               
               
                   
                 Mean 
                 65.5 
                 87.7 
                 200.0 
                 2824.6 
               
               
                   
                 STDER 
                 0.3 
                 8.1 
                 0.0 
                 22.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 65.0 
                 82.0 
                 200.0 
                 2785.6 
               
               
                 YY101 0.05 nM 
                 2nd 
                 70.0 
                 87.0 
                 200.0 
                 3174.7 
               
               
                   
                 3rd 
                 68.5 
                 96.0 
                 200.0 
                 3058.1 
               
               
                   
                 Mean 
                 67.8 
                 88.3 
                 200.0 
                 3006.1 
               
               
                   
                 STDER 
                 1.5 
                 4.1 
                 0.0 
                 115.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 64.5 
                 76.0 
                 200.0 
                 2746.7 
               
               
                 YY101 0.005 nM 
                 2nd 
                 64.0 
                 61.0 
                 200.0 
                 2707.7 
               
               
                   
                 3rd 
                 62.0 
                 68.0 
                 200.0 
                 2551.7 
               
               
                   
                 Mean 
                 63.5 
                 68.3 
                 200.0 
                 2668.7 
               
               
                   
                 STDER 
                 0.8 
                 4.3 
                 0.0 
                 59.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 67.0 
                 85.0 
                 200.0 
                 2941.4 
               
               
                 YDE011 500 nM 
                 2nd 
                 37.0 
                 95.0 
                 200.0 
                 579.2 
               
               
                   
                 3rd 
                 51.5 
                 86.0 
                 200.0 
                 1729.4 
               
               
                   
                 Mean 
                 51.8 
                 88.7 
                 200.0 
                 1750.0 
               
               
                   
                 STDER 
                 8.7 
                 3.2 
                 0.0 
                 682.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 56.0 
                 111.0 
                 200.0 
                 2082.5 
               
               
                 YDE011 50 nM 
                 2nd 
                 57.0 
                 71.0 
                 200.0 
                 2160.8 
               
               
                   
                 3rd 
                 38.0 
                 81.0 
                 200.0 
                 659.4 
               
               
                   
                 Mean 
                 50.3 
                 87.7 
                 200.0 
                 1634.3 
               
               
                   
                 STDER 
                 6.2 
                 12.0 
                 0.0 
                 487.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 49.0 
                 80.0 
                 200.0 
                 1532.6 
               
               
                 YDE011 5 nM 
                 2nd 
                 50.0 
                 83.0 
                 200.0 
                 1611.3 
               
               
                   
                 3rd 
                 62.0 
                 100.0 
                 200.0 
                 2551.7 
               
               
                   
                 Mean 
                 53.7 
                 87.7 
                 200.0 
                 1898.5 
               
               
                   
                 STDER 
                 4.2 
                 6.2 
                 0.0 
                 327.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 58.0 
                 85.0 
                 200.0 
                 2239.1 
               
               
                 YDE011 0.5 nM 
                 2nd 
                 54.0 
                 82.0 
                 200.0 
                 1925.7 
               
               
                   
                 3rd 
                 57.5 
                 92.0 
                 200.0 
                 2200.0 
               
               
                   
                 Mean 
                 56.5 
                 86.3 
                 200.0 
                 2121.6 
               
               
                   
                 STDER 
                 1.3 
                 3.0 
                 0.0 
                 98.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 64.0 
                 90.0 
                 200.0 
                 2707.7 
               
               
                 YDE011 0.05 nM 
                 2nd 
                 61.0 
                 86.0 
                 200.0 
                 2473.6 
               
               
                   
                 3rd 
                 65.5 
                 102.0 
                 200.0 
                 2824.6 
               
               
                   
                 Mean 
                 63.5 
                 92.7 
                 200.0 
                 2668.6 
               
               
                   
                 STDER 
                 1.3 
                 4.8 
                 0.0 
                 103.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 58.0 
                 79.0 
                 200.0 
                 2239.1 
               
               
                 YDE011 0.005 nM 
                 2nd 
                 56.0 
                 78.0 
                 200.0 
                 2082.5 
               
               
                   
                 3rd 
                 64.0 
                 100.0 
                 200.0 
                 2707.7 
               
               
                   
                 Mean 
                 59.3 
                 85.7 
                 200.0 
                 2343.1 
               
               
                   
                 STDER 
                 2.4 
                 7.2 
                 0.0 
                 187.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 55.0 
                 95.0 
                 200.0 
                 2004.2 
               
               
                 YDE043 500 nM 
                 2nd 
                 56.0 
                 84.0 
                 200.0 
                 2082.5 
               
               
                   
                 3rd 
                 58.0 
                 87.0 
                 200.0 
                 2239.1 
               
               
                   
                 Mean 
                 56.3 
                 88.7 
                 200.0 
                 2108.6 
               
               
                   
                 STDER 
                 0.9 
                 3.3 
                 0.0 
                 69.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 62.0 
                 90.0 
                 200.0 
                 2551.7 
               
               
                 YDE043 50 nM 
                 2nd 
                 60.0 
                 102.0 
                 200.0 
                 2395.5 
               
               
                   
                 3rd 
                 58.0 
                 87.0 
                 200.0 
                 2239.1 
               
               
                   
                 Mean 
                 60.0 
                 93.0 
                 200.0 
                 2395.4 
               
               
                   
                 STDER 
                 1.2 
                 4.6 
                 0.0 
                 90.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 65.0 
                 76.0 
                 200.0 
                 2785.6 
               
               
                 YDE043 5 nM 
                 2nd 
                 60.0 
                 77.0 
                 200.0 
                 2395.5 
               
               
                   
                 3rd 
                 61.0 
                 103.0 
                 200.0 
                 2473.6 
               
               
                   
                 Mean 
                 62.0 
                 85.3 
                 200.0 
                 2551.6 
               
               
                   
                 STDER 
                 1.5 
                 8.8 
                 0.0 
                 119.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 56.0 
                 83.0 
                 200.0 
                 2082.5 
               
               
                 YDE043 0.5 nM 
                 2nd 
                 59.0 
                 98.0 
                 200.0 
                 2317.3 
               
               
                   
                 3rd 
                 56.5 
                 92.0 
                 200.0 
                 2121.7 
               
               
                   
                 Mean 
                 57.2 
                 91.0 
                 200.0 
                 2173.8 
               
               
                   
                 STDER 
                 0.9 
                 4.4 
                 0.0 
                 72.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 55.0 
                 72.0 
                 200.0 
                 2004.2 
               
               
                 YDE043 0.05 nM 
                 2nd 
                 58.0 
                 80.0 
                 200.0 
                 2239.1 
               
               
                   
                 3rd 
                 58.0 
                 88.0 
                 200.0 
                 2239.1 
               
               
                   
                 Mean 
                 57.0 
                 80.0 
                 200.0 
                 2160.8 
               
               
                   
                 STDER 
                 1.0 
                 4.6 
                 0.0 
                 78.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 58.0 
                 82.0 
                 200.0 
                 2239.1 
               
               
                 YDE043 0.005 nM 
                 2nd 
                 59.0 
                 85.0 
                 200.0 
                 2317.3 
               
               
                   
                 3rd 
                 68.5 
                 66.0 
                 200.0 
                 3058.1 
               
               
                   
                 Mean 
                 61.8 
                 77.7 
                 200.0 
                 2538.2 
               
               
                   
                 STDER 
                 3.3 
                 5.9 
                 0.0 
                 260.9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 Bead name: IL-6 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concentration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 Dilution 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 20.5 
                 78.0 
                 1.0 
                 &lt;2.65 
               
               
                   
                 2nd 
                 20.0 
                 108.0 
                 1.0 
                 &lt;2.65 
               
               
                   
                 Mean 
                 20.3 
                 93.0 
                 1.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.3 
                 15.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 22.5 
                 94.0 
                 200.0 
                 16.1 
               
               
                   
                 2nd 
                 22.0 
                 93.0 
                 200.0 
                 5.0 
               
               
                   
                 3rd 
                 22.0 
                 111.0 
                 200.0 
                 5.0 
               
               
                   
                 Mean 
                 22.2 
                 99.3 
                 200.0 
                 8.7 
               
               
                   
                 STDER 
                 0.2 
                 5.8 
                 0.0 
                 3.7 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 1407.0 
                 93.0 
                 200.0 
                 32871.0 
               
               
                   
                 2nd 
                 1182.0 
                 74.0 
                 200.0 
                 27467.5 
               
               
                   
                 3rd 
                 1126.5 
                 114.0 
                 200.0 
                 26138.3 
               
               
                   
                 Mean 
                 1238.5 
                 93.7 
                 200.0 
                 28825.6 
               
               
                   
                 STDER 
                 85.8 
                 11.6 
                 0.0 
                 2058.8 
               
               
                 PBMC + YY101 500 nM 
                 1st 
                 21.0 
                 114.0 
                 200.0 
                 &lt;2.65 
               
               
                   
                 2nd 
                 21.0 
                 122.0 
                 200.0 
                 &lt;2.65 
               
               
                   
                 Mean 
                 21.0 
                 118.0 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 4.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + YY101 50 nM 
                 1st 
                 22.0 
                 84.0 
                 200.0 
                 5.0 
               
               
                   
                 2nd 
                 20.0 
                 106.0 
                 200.0 
                 &lt;2.65 
               
               
                   
                 Mean 
                 21.0 
                 95.0 
                 200.0 
                 5.0 
               
               
                   
                 STDER 
                 1.0 
                 11.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + YY101 5 nM 
                 1st 
                 22.0 
                 103.0 
                 200.0 
                 5.0 
               
               
                   
                 2nd 
                 22.0 
                 100.0 
                 200.0 
                 5.0 
               
               
                   
                 Mean 
                 22.0 
                 101.5 
                 200.0 
                 5.0 
               
               
                   
                 STDER 
                 0.0 
                 1.5 
                 0.0 
                 0.0 
               
               
                 PBMC + YY101 0.5 nM 
                 1st 
                 22.0 
                 99.0 
                 200.0 
                 5.0 
               
               
                   
                 2nd 
                 23.0 
                 101.0 
                 200.0 
                 27.3 
               
               
                   
                 Mean 
                 22.5 
                 100.0 
                 200.0 
                 16.2 
               
               
                   
                 STDER 
                 0.5 
                 1.0 
                 0.0 
                 11.1 
               
               
                 PBMC + YY101 0.05 uM 
                 1st 
                 28.0 
                 80.0 
                 200.0 
                 140.1 
               
               
                   
                 2nd 
                 20.0 
                 106.0 
                 200.0 
                 &lt;2.65 
               
               
                   
                 Mean 
                 24.0 
                 93.0 
                 200.0 
                 140.1 
               
               
                   
                 STDER 
                 4.0 
                 13.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1422.0 
                 94.0 
                 200.0 
                 33232.1 
               
               
                 Xiidra 500 nM 
                 2nd 
                 1420.0 
                 84.0 
                 200.0 
                 33183.9 
               
               
                   
                 Mean 
                 1421.0 
                 89.0 
                 200.0 
                 33208.0 
               
               
                   
                 STDER 
                 1.0 
                 5.0 
                 0.0 
                 24.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1365.0 
                 97.0 
                 200.0 
                 31860.4 
               
               
                 Xiidra 50 nM 
                 2nd 
                 1427.5 
                 122.0 
                 200.0 
                 33364.6 
               
               
                   
                 Mean 
                 1396.3 
                 109.5 
                 200.0 
                 32612.5 
               
               
                   
                 STDER 
                 31.3 
                 12.5 
                 0.0 
                 752.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1234.5 
                 114.0 
                 200.0 
                 28726.1 
               
               
                 Xiidra 5 nM 
                 2nd 
                 1306.0 
                 109.0 
                 200.0 
                 30442.3 
               
               
                   
                 Mean 
                 1270.3 
                 111.5 
                 200.0 
                 29584.2 
               
               
                   
                 STDER 
                 35.8 
                 2.5 
                 0.0 
                 858.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1094.5 
                 108.0 
                 200.0 
                 25372.4 
               
               
                 Xiidra 0.5 nM 
                 2nd 
                 1188.5 
                 102.0 
                 200.0 
                 27623.3 
               
               
                   
                 Mean 
                 1141.5 
                 105.0 
                 200.0 
                 26497.8 
               
               
                   
                 STDER 
                 47.0 
                 3.0 
                 0.0 
                 1125.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1112.0 
                 127.0 
                 200.0 
                 25791.2 
               
               
                 Xiidra 0.05 nM 
                 2nd 
                 1129.0 
                 104.0 
                 200.0 
                 26198.1 
               
               
                   
                 Mean 
                 1120.5 
                 115.5 
                 200.0 
                 25994.6 
               
               
                   
                 STDER 
                 8.5 
                 11.5 
                 0.0 
                 203.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1131.5 
                 108.0 
                 200.0 
                 26257.9 
               
               
                 Xiidra 0.005 nM 
                 2nd 
                 1091.0 
                 94.0 
                 200.0 
                 25288.7 
               
               
                   
                 Mean 
                 1111.3 
                 101.0 
                 200.0 
                 25773.3 
               
               
                   
                 STDER 
                 20.3 
                 7.0 
                 0.0 
                 484.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 749.0 
                 65.0 
                 200.0 
                 17129.3 
               
               
                 YY101 500 nM 
                 2nd 
                 775.0 
                 118.0 
                 200.0 
                 17748.1 
               
               
                   
                 3rd 
                 727.5 
                 84.0 
                 200.0 
                 16617.9 
               
               
                   
                 Mean 
                 750.5 
                 89.0 
                 200.0 
                 17165.1 
               
               
                   
                 STDER 
                 13.7 
                 15.5 
                 0.0 
                 326.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 715.0 
                 87.0 
                 200.0 
                 16320.6 
               
               
                 YY101 50 nM 
                 2nd 
                 730.5 
                 112.0 
                 200.0 
                 16689.2 
               
               
                   
                 3rd 
                 780.5 
                 98.0 
                 200.0 
                 17879.0 
               
               
                   
                 Mean 
                 742.0 
                 99.0 
                 200.0 
                 16962.9 
               
               
                   
                 STDER 
                 19.8 
                 7.2 
                 0.0 
                 470.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 727.0 
                 95.0 
                 200.0 
                 16606.0 
               
               
                 YY101 5 nM 
                 2nd 
                 720.0 
                 84.0 
                 200.0 
                 16439.5 
               
               
                   
                 3rd 
                 813.0 
                 105.0 
                 200.0 
                 18652.8 
               
               
                   
                 Mean 
                 753.3 
                 94.7 
                 200.0 
                 17232.8 
               
               
                   
                 STDER 
                 29.9 
                 6.1 
                 0.0 
                 711.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 788.5 
                 94.0 
                 200.0 
                 18069.4 
               
               
                 YY101 0.5 nM 
                 2nd 
                 754.0 
                 98.0 
                 200.0 
                 17248.3 
               
               
                   
                 3rd 
                 816.5 
                 96.0 
                 200.0 
                 18736.2 
               
               
                   
                 Mean 
                 786.3 
                 96.0 
                 200.0 
                 18018.0 
               
               
                   
                 STDER 
                 18.1 
                 1.2 
                 0.0 
                 430.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 769.0 
                 94.0 
                 200.0 
                 17605.3 
               
               
                 YY101 0.05 nM 
                 2nd 
                 832.0 
                 99.0 
                 200.0 
                 19105.4 
               
               
                   
                 3rd 
                 803.0 
                 87.0 
                 200.0 
                 18414.7 
               
               
                   
                 Mean 
                 801.3 
                 93.3 
                 200.0 
                 18375.1 
               
               
                   
                 STDER 
                 18.2 
                 3.5 
                 0.0 
                 433.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 817.5 
                 106.0 
                 200.0 
                 18760.0 
               
               
                 YY101 0.005 nM 
                 2nd 
                 772.0 
                 85.0 
                 200.0 
                 17676.7 
               
               
                   
                 3rd 
                 775.0 
                 97.0 
                 200.0 
                 17748.1 
               
               
                   
                 Mean 
                 788.2 
                 96.0 
                 200.0 
                 18061.6 
               
               
                   
                 STDER 
                 14.7 
                 6.1 
                 0.0 
                 349.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 843.0 
                 88.0 
                 200.0 
                 19367.5 
               
               
                 YDE011 500 nM 
                 2nd 
                 422.5 
                 96.0 
                 200.0 
                 9383.5 
               
               
                   
                 3rd 
                 657.5 
                 98.0 
                 200.0 
                 14953.9 
               
               
                   
                 Mean 
                 641.0 
                 94.0 
                 200.0 
                 14568.3 
               
               
                   
                 STDER 
                 121.7 
                 3.1 
                 0.0 
                 2888.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 732.0 
                 132.0 
                 200.0 
                 16724.9 
               
               
                 YDE011 50 nM 
                 2nd 
                 754.0 
                 97.0 
                 200.0 
                 17248.3 
               
               
                   
                 3rd 
                 444.0 
                 97.0 
                 200.0 
                 9892.0 
               
               
                   
                 Mean 
                 643.3 
                 108.7 
                 200.0 
                 14621.7 
               
               
                   
                 STDER 
                 99.9 
                 11.7 
                 0.0 
                 2369.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 537.0 
                 93.0 
                 200.0 
                 12094.3 
               
               
                 YDE011 5 nM 
                 2nd 
                 573.0 
                 105.0 
                 200.0 
                 12947.9 
               
               
                   
                 3rd 
                 684.0 
                 93.0 
                 200.0 
                 15583.6 
               
               
                   
                 Mean 
                 598.0 
                 97.0 
                 200.0 
                 13541.9 
               
               
                   
                 STDER 
                 44.2 
                 4.0 
                 0.0 
                 1050.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 673.0 
                 99.0 
                 200.0 
                 15322.2 
               
               
                 YDE011 0.5 nM 
                 2nd 
                 687.0 
                 97.0 
                 200.0 
                 15654.9 
               
               
                   
                 3rd 
                 676.0 
                 104.0 
                 200.0 
                 15393.5 
               
               
                   
                 Mean 
                 678.7 
                 100.0 
                 200.0 
                 15456.8 
               
               
                   
                 STDER 
                 4.3 
                 2.1 
                 0.0 
                 101.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 821.0 
                 96.0 
                 200.0 
                 18843.4 
               
               
                 YDE011 0.05 nM 
                 2nd 
                 800.0 
                 81.0 
                 200.0 
                 18343.3 
               
               
                   
                 3rd 
                 810.0 
                 84.0 
                 200.0 
                 18581.4 
               
               
                   
                 Mean 
                 810.3 
                 87.0 
                 200.0 
                 18589.3 
               
               
                   
                 STDER 
                 6.1 
                 4.6 
                 0.0 
                 144.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 737.0 
                 73.0 
                 200.0 
                 16843.8 
               
               
                 YDE011 0.005 nM 
                 2nd 
                 670.0 
                 89.0 
                 200.0 
                 15250.9 
               
               
                   
                 3rd 
                 712.0 
                 114.0 
                 200.0 
                 16249.2 
               
               
                   
                 Mean 
                 706.3 
                 92.0 
                 200.0 
                 16114.6 
               
               
                   
                 STDER 
                 19.5 
                 11.9 
                 0.0 
                 464.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 612.0 
                 95.0 
                 200.0 
                 13873.4 
               
               
                 YDE043 500 nM 
                 2nd 
                 612.0 
                 95.0 
                 200.0 
                 13873.4 
               
               
                   
                 3rd 
                 654.5 
                 86.0 
                 200.0 
                 14882.6 
               
               
                   
                 Mean 
                 626.2 
                 92.0 
                 200.0 
                 14209.8 
               
               
                   
                 STDER 
                 14.2 
                 3.0 
                 0.0 
                 336.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 742.0 
                 89.0 
                 200.0 
                 16962.8 
               
               
                 YDE043 50 nM 
                 2nd 
                 725.5 
                 94.0 
                 200.0 
                 16570.3 
               
               
                   
                 3rd 
                 645.5 
                 118.0 
                 200.0 
                 14668.8 
               
               
                   
                 Mean 
                 704.3 
                 100.3 
                 200.0 
                 16067.3 
               
               
                   
                 STDER 
                 29.8 
                 9.0 
                 0.0 
                 708.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 717.5 
                 80.0 
                 200.0 
                 16380.0 
               
               
                 YDE043 5 nM 
                 2nd 
                 753.0 
                 73.0 
                 200.0 
                 17224.5 
               
               
                   
                 3rd 
                 698.0 
                 121.0 
                 200.0 
                 15916.4 
               
               
                   
                 Mean 
                 722.8 
                 91.3 
                 200.0 
                 16507.0 
               
               
                   
                 STDER 
                 16.1 
                 15.0 
                 0.0 
                 382.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 673.0 
                 83.0 
                 200.0 
                 15322.2 
               
               
                 YDE043 0.5 nM 
                 2nd 
                 663.0 
                 101.0 
                 200.0 
                 15084.6 
               
               
                   
                 3rd 
                 618.5 
                 92.0 
                 200.0 
                 14027.7 
               
               
                   
                 Mean 
                 651.5 
                 92.0 
                 200.0 
                 14811.5 
               
               
                   
                 STDER 
                 16.8 
                 5.2 
                 0.0 
                 397.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 591.5 
                 90.0 
                 200.0 
                 13386.9 
               
               
                 YDE043 0.05 nM 
                 2nd 
                 647.5 
                 96.0 
                 200.0 
                 14716.3 
               
               
                   
                 3rd 
                 645.0 
                 99.0 
                 200.0 
                 14657.0 
               
               
                   
                 Mean 
                 628.0 
                 95.0 
                 200.0 
                 14253.4 
               
               
                   
                 STDER 
                 18.3 
                 2.6 
                 0.0 
                 433.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 690.0 
                 89.0 
                 200.0 
                 15726.2 
               
               
                 YDE043 0.005 nM 
                 2nd 
                 734.0 
                 87.0 
                 200.0 
                 16772.5 
               
               
                   
                 3rd 
                 892.0 
                 99.0 
                 200.0 
                 20535.6 
               
               
                   
                 Mean 
                 772.0 
                 91.7 
                 200.0 
                 17678.1 
               
               
                   
                 STDER 
                 61.3 
                 3.7 
                 0.0 
                 1460.3 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Bead name: IL-8/CXCL8 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concen- 
               
               
                   
                   
                   
                   
                 Dilu- 
                 tration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 tion 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 32.0 
                 76.0 
                 1.0 
                 0.3 
               
               
                   
                 2nd 
                 30.0 
                 90.0 
                 1.0 
                 0.2 
               
               
                   
                 Mean 
                 31.0 
                 83.0 
                 1.0 
                 0.3 
               
               
                   
                 STDER 
                 1.0 
                 7.0 
                 0.0 
                 0.1 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 287.5 
                 78.0 
                 200.0 
                 3955.9 
               
               
                   
                 2nd 
                 110.5 
                 86.0 
                 200.0 
                 1155.1 
               
               
                   
                 3rd 
                 113.0 
                 91.0 
                 200.0 
                 1192.3 
               
               
                   
                 Mean 
                 170.3 
                 85.0 
                 200.0 
                 2101.1 
               
               
                   
                 STDER 
                 58.6 
                 3.8 
                 0.0 
                 927.5 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 4811.0 
                 82.0 
                 200.0 
                 100889.1 
               
               
                   
                 2nd 
                 4193.0 
                 91.0 
                 200.0 
                 84243.2 
               
               
                   
                 3rd 
                 4083.0 
                 83.0 
                 200.0 
                 81501.2 
               
               
                   
                 Mean 
                 4362.3 
                 85.3 
                 200.0 
                 88877.9 
               
               
                   
                 STDER 
                 226.6 
                 2.8 
                 0.0 
                 6057.6 
               
               
                 PBMC + YY101 500 nM 
                 1st 
                 120.0 
                 87.0 
                 200.0 
                 1297.1 
               
               
                   
                 2nd 
                 165.0 
                 93.0 
                 200.0 
                 1986.4 
               
               
                   
                 Mean 
                 142.5 
                 90.0 
                 200.0 
                 1641.8 
               
               
                   
                 STDER 
                 22.5 
                 3.0 
                 0.0 
                 344.6 
               
               
                 PBMC + YY101 50 nM 
                 1st 
                 166.5 
                 76.0 
                 200.0 
                 2009.8 
               
               
                   
                 2nd 
                 117.0 
                 85.0 
                 200.0 
                 1252.1 
               
               
                   
                 Mean 
                 141.8 
                 80.5 
                 200.0 
                 1630.9 
               
               
                   
                 STDER 
                 24.8 
                 4.5 
                 0.0 
                 378.8 
               
               
                 PBMC + YY101 5 nM 
                 1st 
                 281.0 
                 93.0 
                 200.0 
                 3848.8 
               
               
                   
                 2nd 
                 151.0 
                 87.0 
                 200.0 
                 1769.4 
               
               
                   
                 Mean 
                 216.0 
                 90.0 
                 200.0 
                 2809.1 
               
               
                   
                 STDER 
                 65.0 
                 3.0 
                 0.0 
                 1039.7 
               
               
                 PBMC + YY101 0.5 nM 
                 1st 
                 164.0 
                 81.0 
                 200.0 
                 1970.8 
               
               
                   
                 2nd 
                 368.0 
                 89.0 
                 200.0 
                 5300.9 
               
               
                   
                 Mean 
                 266.0 
                 85.0 
                 200.0 
                 3635.9 
               
               
                   
                 STDER 
                 102.0 
                 4.0 
                 0.0 
                 1665.0 
               
               
                 PBMC + YY101 0.05 
                 1st 
                 836.5 
                 74.0 
                 200.0 
                 13586.7 
               
               
                 uM 
                 2nd 
                 207.0 
                 97.0 
                 200.0 
                 2648.7 
               
               
                   
                 Mean 
                 521.8 
                 85.5 
                 200.0 
                 8117.7 
               
               
                   
                 STDER 
                 314.8 
                 11.5 
                 0.0 
                 5469.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 4811.0 
                 90.0 
                 200.0 
                 100889.1 
               
               
                 Xiidra 500 nM 
                 2nd 
                 4424.0 
                 73.0 
                 200.0 
                 90193.3 
               
               
                   
                 Mean 
                 4617.5 
                 81.5 
                 200.0 
                 95541.2 
               
               
                   
                 STDER 
                 193.5 
                 8.5 
                 0.0 
                 5347.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 4604.0 
                 66.0 
                 200.0 
                 95040.0 
               
               
                 Xiidra 50 nM 
                 2nd 
                 4553.0 
                 99.0 
                 200.0 
                 93646.0 
               
               
                   
                 Mean 
                 4578.5 
                 82.5 
                 200.0 
                 94343.0 
               
               
                   
                 STDER 
                 25.5 
                 16.5 
                 0.0 
                 697.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 4476.0 
                 84.0 
                 200.0 
                 91572.9 
               
               
                 Xiidra 5 nM 
                 2nd 
                 4697.5 
                 74.0 
                 200.0 
                 97642.3 
               
               
                   
                 Mean 
                 4586.8 
                 79.0 
                 200.0 
                 94607.6 
               
               
                   
                 STDER 
                 110.8 
                 5.0 
                 0.0 
                 3034.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 4067.5 
                 78.0 
                 200.0 
                 81119.1 
               
               
                 Xiidra 0.5 nM 
                 2nd 
                 4450.5 
                 96.0 
                 200.0 
                 90894.4 
               
               
                   
                 Mean 
                 4259.0 
                 87.0 
                 200.0 
                 86006.8 
               
               
                   
                 STDER 
                 191.5 
                 9.0 
                 0.0 
                 4887.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 4169.0 
                 109.0 
                 200.0 
                 83640.3 
               
               
                 Xiidra 0.05 nM 
                 2nd 
                 4413.0 
                 73.0 
                 200.0 
                 89903.5 
               
               
                   
                 Mean 
                 4291.0 
                 91.0 
                 200.0 
                 86771.9 
               
               
                   
                 STDER 
                 122.0 
                 18.0 
                 0.0 
                 3131.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 4154.0 
                 91.0 
                 200.0 
                 83264.8 
               
               
                 Xiidra 0.005 nM 
                 2nd 
                 4103.5 
                 100.0 
                 200.0 
                 82008.2 
               
               
                   
                 Mean 
                 4128.8 
                 95.5 
                 200.0 
                 82636.5 
               
               
                   
                 STDER 
                 25.3 
                 4.5 
                 0.0 
                 628.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 3175.0 
                 77.0 
                 200.0 
                 60492.6 
               
               
                 YY101 500 nM 
                 2nd 
                 3150.0 
                 81.0 
                 200.0 
                 59945.6 
               
               
                   
                 3rd 
                 2895.0 
                 68.0 
                 200.0 
                 54438.6 
               
               
                   
                 Mean 
                 3073.3 
                 75.3 
                 200.0 
                 58292.3 
               
               
                   
                 STDER 
                 89.5 
                 3.8 
                 0.0 
                 1933.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2910.0 
                 67.0 
                 200.0 
                 54759.1 
               
               
                 YY101 50 nM 
                 2nd 
                 2830.5 
                 114.0 
                 200.0 
                 53065.0 
               
               
                   
                 3rd 
                 2978.0 
                 91.0 
                 200.0 
                 56217.2 
               
               
                   
                 Mean 
                 2906.2 
                 90.7 
                 200.0 
                 54680.4 
               
               
                   
                 STDER 
                 42.6 
                 13.6 
                 0.0 
                 910.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2948.5 
                 96.0 
                 200.0 
                 55583.6 
               
               
                 YY101 5 nM 
                 2nd 
                 2967.0 
                 77.0 
                 200.0 
                 55980.8 
               
               
                   
                 3rd 
                 3170.0 
                 75.0 
                 200.0 
                 60383.1 
               
               
                   
                 Mean 
                 3028.5 
                 82.7 
                 200.0 
                 57315.8 
               
               
                   
                 STDER 
                 71.0 
                 6.7 
                 0.0 
                 1537.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 3194.0 
                 89.0 
                 200.0 
                 60909.2 
               
               
                 YY101 0.5 nM 
                 2nd 
                 3056.0 
                 95.0 
                 200.0 
                 57900.7 
               
               
                   
                 3rd 
                 3241.0 
                 61.0 
                 200.0 
                 61943.2 
               
               
                   
                 Mean 
                 3163.7 
                 81.7 
                 200.0 
                 60251.0 
               
               
                   
                 STDER 
                 55.5 
                 10.5 
                 0.0 
                 1212.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 3031.0 
                 88.0 
                 200.0 
                 57359.8 
               
               
                 YY101 0.05 nM 
                 2nd 
                 3177.0 
                 77.0 
                 200.0 
                 60536.4 
               
               
                   
                 3rd 
                 3247.0 
                 77.0 
                 200.0 
                 62075.6 
               
               
                   
                 Mean 
                 3151.7 
                 80.7 
                 200.0 
                 59990.6 
               
               
                   
                 STDER 
                 63.6 
                 3.7 
                 0.0 
                 1388.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 3223.0 
                 64.0 
                 200.0 
                 61546.6 
               
               
                 YY101 0.005 nM 
                 2nd 
                 3009.0 
                 78.0 
                 200.0 
                 56884.9 
               
               
                   
                 3rd 
                 3015.0 
                 75.0 
                 200.0 
                 57014.3 
               
               
                   
                 Mean 
                 3082.3 
                 72.3 
                 200.0 
                 58481.9 
               
               
                   
                 STDER 
                 70.4 
                 4.3 
                 0.0 
                 1532.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 3436.0 
                 70.0 
                 200.0 
                 66288.2 
               
               
                 YDE011 500 nM 
                 2nd 
                 1788.0 
                 97.0 
                 200.0 
                 31741.3 
               
               
                   
                 3rd 
                 2708.0 
                 79.0 
                 200.0 
                 50476.2 
               
               
                   
                 Mean 
                 2644.0 
                 82.0 
                 200.0 
                 49501.9 
               
               
                   
                 STDER 
                 476.8 
                 7.9 
                 0.0 
                 9984.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2885.5 
                 86.0 
                 200.0 
                 54235.8 
               
               
                 YDE011 50 nM 
                 2nd 
                 2934.0 
                 74.0 
                 200.0 
                 55272.7 
               
               
                   
                 3rd 
                 1763.5 
                 68.0 
                 200.0 
                 31257.8 
               
               
                   
                 Mean 
                 2527.7 
                 76.0 
                 200.0 
                 46922.1 
               
               
                   
                 STDER 
                 382.3 
                 5.3 
                 0.0 
                 7837.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2473.5 
                 80.0 
                 200.0 
                 45587.7 
               
               
                 YDE011 5 nM 
                 2nd 
                 2353.0 
                 76.0 
                 200.0 
                 43107.6 
               
               
                   
                 3rd 
                 2728.5 
                 94.0 
                 200.0 
                 50907.7 
               
               
                   
                 Mean 
                 2518.3 
                 83.3 
                 200.0 
                 46534.3 
               
               
                   
                 STDER 
                 110.7 
                 5.5 
                 0.0 
                 2300.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2824.0 
                 110.0 
                 200.0 
                 52927.0 
               
               
                 YDE011 0.5 nM 
                 2nd 
                 2793.0 
                 107.0 
                 200.0 
                 52269.8 
               
               
                   
                 3rd 
                 2767.5 
                 88.0 
                 200.0 
                 51730.5 
               
               
                   
                 Mean 
                 2794.8 
                 101.7 
                 200.0 
                 52309.1 
               
               
                   
                 STDER 
                 16.3 
                 6.9 
                 0.0 
                 346.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 3224.0 
                 105.0 
                 200.0 
                 61568.6 
               
               
                 YDE011 0.05 nM 
                 2nd 
                 3187.0 
                 75.0 
                 200.0 
                 60755.7 
               
               
                   
                 3rd 
                 3203.0 
                 82.0 
                 200.0 
                 61106.8 
               
               
                   
                 Mean 
                 3204.7 
                 87.3 
                 200.0 
                 61143.7 
               
               
                   
                 STDER 
                 10.7 
                 9.1 
                 0.0 
                 235.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2963.5 
                 76.0 
                 200.0 
                 55905.6 
               
               
                 YDE011 0.005 nM 
                 2nd 
                 2780.0 
                 84.0 
                 200.0 
                 51994.7 
               
               
                   
                 3rd 
                 2980.0 
                 103.0 
                 200.0 
                 56260.2 
               
               
                   
                 Mean 
                 2907.8 
                 87.7 
                 200.0 
                 54720.2 
               
               
                   
                 STDER 
                 64.1 
                 8.0 
                 0.0 
                 1366.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2752.5 
                 58.0 
                 200.0 
                 51413.7 
               
               
                 YDE043 500 nM 
                 2nd 
                 2670.0 
                 97.0 
                 200.0 
                 49678.2 
               
               
                   
                 3rd 
                 2861.0 
                 86.0 
                 200.0 
                 53713.6 
               
               
                   
                 Mean 
                 2761.2 
                 80.3 
                 200.0 
                 51601.8 
               
               
                   
                 STDER 
                 55.3 
                 11.6 
                 0.0 
                 1168.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 3049.5 
                 72.0 
                 200.0 
                 57759.9 
               
               
                 YDE043 50 nM 
                 2nd 
                 3090.0 
                 89.0 
                 200.0 
                 58638.2 
               
               
                   
                 3rd 
                 2701.0 
                 90.0 
                 200.0 
                 50329.0 
               
               
                   
                 Mean 
                 2946.8 
                 83.7 
                 200.0 
                 55575.7 
               
               
                   
                 STDER 
                 123.5 
                 5.8 
                 0.0 
                 2635.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2993.0 
                 69.0 
                 200.0 
                 56540.0 
               
               
                 YDE043 5 nM 
                 2nd 
                 3073.0 
                 69.0 
                 200.0 
                 58269.2 
               
               
                   
                 3rd 
                 2846.0 
                 105.0 
                 200.0 
                 53394.4 
               
               
                   
                 Mean 
                 2970.7 
                 81.0 
                 200.0 
                 56067.9 
               
               
                   
                 STDER 
                 66.5 
                 12.0 
                 0.0 
                 1426.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2928.0 
                 66.0 
                 200.0 
                 55144.2 
               
               
                 YDE043 0.5 nM 
                 2nd 
                 2761.0 
                 89.0 
                 200.0 
                 51593.2 
               
               
                   
                 3rd 
                 2697.0 
                 81.0 
                 200.0 
                 50244.9 
               
               
                   
                 Mean 
                 2795.3 
                 78.7 
                 200.0 
                 52327.4 
               
               
                   
                 STDER 
                 68.9 
                 6.7 
                 0.0 
                 1461.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2613.0 
                 77.0 
                 200.0 
                 48485.5 
               
               
                 YDE043 0.05 nM 
                 2nd 
                 2832.5 
                 60.0 
                 200.0 
                 53107.5 
               
               
                   
                 3rd 
                 2773.0 
                 89.0 
                 200.0 
                 51846.7 
               
               
                   
                 Mean 
                 2739.5 
                 75.3 
                 200.0 
                 51146.6 
               
               
                   
                 STDER 
                 65.5 
                 8.4 
                 0.0 
                 1379.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2977.5 
                 86.0 
                 200.0 
                 56206.5 
               
               
                 YDE043 0.005 nM 
                 2nd 
                 2856.0 
                 81.0 
                 200.0 
                 53607.2 
               
               
                   
                 3rd 
                 3484.0 
                 72.0 
                 200.0 
                 67372.3 
               
               
                   
                 Mean 
                 3105.8 
                 79.7 
                 200.0 
                 59062.0 
               
               
                   
                 STDER 
                 192.3 
                 4.1 
                 0.0 
                 4222.4 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 Bead name: IL-10 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concen- 
               
               
                   
                   
                   
                   
                 Dilu- 
                 tration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 tion 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 63.5 
                 64.0 
                 1.0 
                 &lt;2.59 
               
               
                   
                 2nd 
                 61.0 
                 61.0 
                 1.0 
                 &lt;2.59 
               
               
                   
                 Mean 
                 62.3 
                 62.5 
                 1.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 1.3 
                 1.5 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 57.0 
                 61.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 2nd 
                 55.0 
                 63.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 3rd 
                 61.0 
                 70.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 Mean 
                 57.7 
                 64.7 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 1.8 
                 2.7 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 134.5 
                 54.0 
                 200.0 
                 1536.4 
               
               
                   
                 2nd 
                 127.5 
                 50.0 
                 200.0 
                 1367.3 
               
               
                   
                 3rd 
                 123.5 
                 60.0 
                 200.0 
                 1269.7 
               
               
                   
                 Mean 
                 128.5 
                 54.7 
                 200.0 
                 1391.1 
               
               
                   
                 STDER 
                 3.2 
                 2.9 
                 0.0 
                 77.9 
               
               
                 PBMC + YY101 500 nM 
                 1st 
                 59.0 
                 50.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 2nd 
                 67.5 
                 50.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 Mean 
                 63.3 
                 50.0 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 4.3 
                 0.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + YY101 50 nM 
                 1st 
                 57.5 
                 70.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 2nd 
                 59.0 
                 57.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 Mean 
                 58.3 
                 63.5 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.8 
                 6.5 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + YY101 5 nM 
                 1st 
                 61.0 
                 72.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 2nd 
                 66.0 
                 70.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 Mean 
                 63.5 
                 71.0 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 2.5 
                 1.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + YY101 0.5 nM 
                 1st 
                 61.0 
                 55.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 2nd 
                 61.0 
                 55.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 Mean 
                 61.0 
                 55.0 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + YY101 0.05 
                 1st 
                 59.5 
                 50.0 
                 200.0 
                 &lt;2.59 
               
               
                 uM 
                 2nd 
                 62.5 
                 64.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 Mean 
                 61.0 
                 57.0 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 1.5 
                 7.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 114.0 
                 67.0 
                 200.0 
                 1034.6 
               
               
                 Xiidra 500 nM 
                 2nd 
                 111.0 
                 62.0 
                 200.0 
                 959.2 
               
               
                   
                 Mean 
                 112.5 
                 64.5 
                 200.0 
                 996.9 
               
               
                   
                 STDER 
                 1.5 
                 2.5 
                 0.0 
                 37.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 126.0 
                 51.0 
                 200.0 
                 1330.8 
               
               
                 Xiidra 50 nM 
                 2nd 
                 147.0 
                 69.0 
                 200.0 
                 1833.9 
               
               
                   
                 Mean 
                 136.5 
                 60.0 
                 200.0 
                 1582.3 
               
               
                   
                 STDER 
                 10.5 
                 9.0 
                 0.0 
                 251.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 135.0 
                 70.0 
                 200.0 
                 1548.4 
               
               
                 Xiidra 5 nM 
                 2nd 
                 139.0 
                 63.0 
                 200.0 
                 1644.1 
               
               
                   
                 Mean 
                 137.0 
                 66.5 
                 200.0 
                 1596.3 
               
               
                   
                 STDER 
                 2.0 
                 3.5 
                 0.0 
                 47.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 114.5 
                 66.0 
                 200.0 
                 1047.1 
               
               
                 Xiidra 0.5 nM 
                 2nd 
                 129.5 
                 72.0 
                 200.0 
                 1415.8 
               
               
                   
                 Mean 
                 122.0 
                 69.0 
                 200.0 
                 1231.5 
               
               
                   
                 STDER 
                 7.5 
                 3.0 
                 0.0 
                 184.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 119.0 
                 78.0 
                 200.0 
                 1158.9 
               
               
                 Xiidra 0.05 nM 
                 2nd 
                 130.0 
                 50.0 
                 200.0 
                 1427.9 
               
               
                   
                 Mean 
                 124.5 
                 64.0 
                 200.0 
                 1293.4 
               
               
                   
                 STDER 
                 5.5 
                 14.0 
                 0.0 
                 134.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 117.5 
                 50.0 
                 200.0 
                 1121.8 
               
               
                 Xiidra 0.005 nM 
                 2nd 
                 128.0 
                 51.0 
                 200.0 
                 1379.4 
               
               
                   
                 Mean 
                 122.8 
                 50.5 
                 200.0 
                 1250.6 
               
               
                   
                 STDER 
                 5.3 
                 0.5 
                 0.0 
                 128.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 106.0 
                 71.0 
                 200.0 
                 832.3 
               
               
                 YY101 500 nM 
                 2nd 
                 108.0 
                 69.0 
                 200.0 
                 883.3 
               
               
                   
                 3rd 
                 103.0 
                 50.0 
                 200.0 
                 755.1 
               
               
                   
                 Mean 
                 105.7 
                 63.3 
                 200.0 
                 823.5 
               
               
                   
                 STDER 
                 1.5 
                 6.7 
                 0.0 
                 37.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 99.0 
                 50.0 
                 200.0 
                 651.0 
               
               
                 YY101 50 nM 
                 2nd 
                 106.5 
                 50.0 
                 200.0 
                 845.0 
               
               
                   
                 3rd 
                 104.5 
                 50.0 
                 200.0 
                 793.8 
               
               
                   
                 Mean 
                 103.3 
                 50.0 
                 200.0 
                 763.3 
               
               
                   
                 STDER 
                 2.2 
                 0.0 
                 0.0 
                 58.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 107.0 
                 65.0 
                 200.0 
                 857.8 
               
               
                 YY101 5 nM 
                 2nd 
                 110.0 
                 52.0 
                 200.0 
                 934.0 
               
               
                   
                 3rd 
                 113.0 
                 50.0 
                 200.0 
                 1009.5 
               
               
                   
                 Mean 
                 110.0 
                 55.7 
                 200.0 
                 933.8 
               
               
                   
                 STDER 
                 1.7 
                 4.7 
                 0.0 
                 43.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 107.5 
                 62.0 
                 200.0 
                 870.5 
               
               
                 YY101 0.5 nM 
                 2nd 
                 101.0 
                 54.0 
                 200.0 
                 703.2 
               
               
                   
                 3rd 
                 101.5 
                 58.0 
                 200.0 
                 716.2 
               
               
                   
                 Mean 
                 103.3 
                 58.0 
                 200.0 
                 763.3 
               
               
                   
                 STDER 
                 2.1 
                 2.3 
                 0.0 
                 53.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 105.0 
                 61.0 
                 200.0 
                 806.6 
               
               
                 YY101 0.05 nM 
                 2nd 
                 117.5 
                 52.0 
                 200.0 
                 1121.8 
               
               
                   
                 3rd 
                 108.0 
                 54.0 
                 200.0 
                 883.3 
               
               
                   
                 Mean 
                 110.2 
                 55.7 
                 200.0 
                 937.2 
               
               
                   
                 STDER 
                 3.8 
                 2.7 
                 0.0 
                 94.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 106.0 
                 52.0 
                 200.0 
                 832.3 
               
               
                 YY101 0.005 nM 
                 2nd 
                 100.0 
                 53.0 
                 200.0 
                 677.1 
               
               
                   
                 3rd 
                 104.0 
                 57.0 
                 200.0 
                 780.9 
               
               
                   
                 Mean 
                 103.3 
                 54.0 
                 200.0 
                 763.4 
               
               
                   
                 STDER 
                 1.8 
                 1.5 
                 0.0 
                 45.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 108.0 
                 70.0 
                 200.0 
                 883.3 
               
               
                 YDE011 500 nM 
                 2nd 
                 60.0 
                 83.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 3rd 
                 83.5 
                 50.0 
                 200.0 
                 225.5 
               
               
                   
                 Mean 
                 83.8 
                 67.7 
                 200.0 
                 554.4 
               
               
                   
                 STDER 
                 13.9 
                 9.6 
                 0.0 
                 328.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 94.0 
                 67.0 
                 200.0 
                 518.2 
               
               
                 YDE011 50 nM 
                 2nd 
                 106.5 
                 50.0 
                 200.0 
                 845.0 
               
               
                   
                 3rd 
                 58.0 
                 53.0 
                 200.0 
                 &lt;2.59 
               
               
                   
                 Mean 
                 86.2 
                 56.7 
                 200.0 
                 681.6 
               
               
                   
                 STDER 
                 14.5 
                 5.2 
                 0.0 
                 163.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 82.0 
                 73.0 
                 200.0 
                 181.1 
               
               
                 YDE011 5 nM 
                 2nd 
                 83.0 
                 56.0 
                 200.0 
                 210.8 
               
               
                   
                 3rd 
                 89.0 
                 61.0 
                 200.0 
                 381.9 
               
               
                   
                 Mean 
                 84.7 
                 63.3 
                 200.0 
                 257.9 
               
               
                   
                 STDER 
                 2.2 
                 5.0 
                 0.0 
                 62.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 90.0 
                 51.0 
                 200.0 
                 409.5 
               
               
                 YDE011 0.5 nM 
                 2nd 
                 86.0 
                 67.0 
                 200.0 
                 297.6 
               
               
                   
                 3rd 
                 90.0 
                 63.0 
                 200.0 
                 409.5 
               
               
                   
                 Mean 
                 88.7 
                 60.3 
                 200.0 
                 372.2 
               
               
                   
                 STDER 
                 1.3 
                 4.8 
                 0.0 
                 37.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 102.0 
                 71.0 
                 200.0 
                 729.2 
               
               
                 YDE011 0.05 nM 
                 2nd 
                 97.5 
                 56.0 
                 200.0 
                 611.5 
               
               
                   
                 3rd 
                 99.0 
                 64.0 
                 200.0 
                 651.0 
               
               
                   
                 Mean 
                 99.5 
                 63.7 
                 200.0 
                 663.9 
               
               
                   
                 STDER 
                 1.3 
                 4.3 
                 0.0 
                 34.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 98.0 
                 60.0 
                 200.0 
                 624.7 
               
               
                 YDE011 0.005 nM 
                 2nd 
                 90.0 
                 57.0 
                 200.0 
                 409.5 
               
               
                   
                 3rd 
                 94.5 
                 50.0 
                 200.0 
                 531.7 
               
               
                   
                 Mean 
                 94.2 
                 55.7 
                 200.0 
                 521.9 
               
               
                   
                 STDER 
                 2.3 
                 3.0 
                 0.0 
                 62.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 92.0 
                 54.0 
                 200.0 
                 464.2 
               
               
                 YDE043 500 nM 
                 2nd 
                 95.0 
                 71.0 
                 200.0 
                 545.0 
               
               
                   
                 3rd 
                 91.0 
                 53.0 
                 200.0 
                 436.9 
               
               
                   
                 Mean 
                 92.7 
                 59.3 
                 200.0 
                 482.0 
               
               
                   
                 STDER 
                 1.2 
                 5.8 
                 0.0 
                 32.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 96.0 
                 64.0 
                 200.0 
                 571.7 
               
               
                 YDE043 50 nM 
                 2nd 
                 97.0 
                 56.0 
                 200.0 
                 598.2 
               
               
                   
                 3rd 
                 94.0 
                 72.0 
                 200.0 
                 518.2 
               
               
                   
                 Mean 
                 95.7 
                 64.0 
                 200.0 
                 562.7 
               
               
                   
                 STDER 
                 0.9 
                 4.6 
                 0.0 
                 23.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 95.0 
                 54.0 
                 200.0 
                 545.0 
               
               
                 YDE043 5 nM 
                 2nd 
                 98.5 
                 50.0 
                 200.0 
                 637.8 
               
               
                   
                 3rd 
                 95.5 
                 80.0 
                 200.0 
                 558.4 
               
               
                   
                 Mean 
                 96.3 
                 61.3 
                 200.0 
                 580.4 
               
               
                   
                 STDER 
                 1.1 
                 9.4 
                 0.0 
                 29.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 91.0 
                 55.0 
                 200.0 
                 436.9 
               
               
                 YDE043 0.5 nM 
                 2nd 
                 93.0 
                 61.0 
                 200.0 
                 491.3 
               
               
                   
                 3rd 
                 98.0 
                 65.0 
                 200.0 
                 624.7 
               
               
                   
                 Mean 
                 94.0 
                 60.3 
                 200.0 
                 517.6 
               
               
                   
                 STDER 
                 2.1 
                 2.9 
                 0.0 
                 55.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 91.0 
                 57.0 
                 200.0 
                 436.9 
               
               
                 YDE043 0.05 nM 
                 2nd 
                 94.0 
                 60.0 
                 200.0 
                 518.2 
               
               
                   
                 3rd 
                 89.0 
                 51.0 
                 200.0 
                 381.9 
               
               
                   
                 Mean 
                 91.3 
                 56.0 
                 200.0 
                 445.7 
               
               
                   
                 STDER 
                 1.5 
                 2.6 
                 0.0 
                 39.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 99.0 
                 62.0 
                 200.0 
                 651.0 
               
               
                 YDE043 0.005 nM 
                 2nd 
                 105.0 
                 57.0 
                 200.0 
                 806.6 
               
               
                   
                 3rd 
                 118.0 
                 68.0 
                 200.0 
                 1134.2 
               
               
                   
                 Mean 
                 107.3 
                 62.3 
                 200.0 
                 863.9 
               
               
                   
                 STDER 
                 5.6 
                 3.2 
                 0.0 
                 142.4 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 Bead name: TNF-alpha 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concen- 
               
               
                   
                   
                   
                   
                 Dilu- 
                 tration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 tion 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 19.0 
                 79.0 
                 1.0 
                 &lt;7.70 
               
               
                   
                 2nd 
                 21.0 
                 89.0 
                 1.0 
                 &lt;7.70 
               
               
                   
                 Mean 
                 20.0 
                 84.0 
                 1.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 1.0 
                 5.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 21.0 
                 76.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 2nd 
                 19.0 
                 77.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 3rd 
                 19.5 
                 100.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 Mean 
                 19.8 
                 84.3 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.6 
                 7.8 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 78.0 
                 72.0 
                 200.0 
                 3554.3 
               
               
                   
                 2nd 
                 66.0 
                 87.0 
                 200.0 
                 2809.1 
               
               
                   
                 3rd 
                 61.0 
                 104.0 
                 200.0 
                 2495.5 
               
               
                   
                 Mean 
                 68.3 
                 87.7 
                 200.0 
                 2953.0 
               
               
                   
                 STDER 
                 5.0 
                 9.2 
                 0.0 
                 314.0 
               
               
                 PBMC + YY101 500 nM 
                 1st 
                 19.5 
                 82.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 2nd 
                 21.0 
                 112.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 Mean 
                 20.3 
                 97.0 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.8 
                 15.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + YY101 50 nM 
                 1st 
                 19.0 
                 63.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 2nd 
                 22.0 
                 70.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 Mean 
                 20.5 
                 66.5 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 1.5 
                 3.5 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + YY101 5 nM 
                 1st 
                 20.0 
                 84.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 2nd 
                 21.0 
                 76.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 Mean 
                 20.5 
                 80.0 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 4.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + YY101 0.5 nM 
                 1st 
                 20.0 
                 85.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 2nd 
                 19.0 
                 77.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 Mean 
                 19.5 
                 81.0 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 4.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + YY101 0.05 
                 1st 
                 21.0 
                 83.0 
                 200.0 
                 &lt;7.70 
               
               
                 uM 
                 2nd 
                 22.0 
                 95.0 
                 200.0 
                 &lt;7.70 
               
               
                   
                 Mean 
                 21.5 
                 89.0 
                 200.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 6.0 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 72.0 
                 71.0 
                 200.0 
                 3183.0 
               
               
                 Xiidra 500 nM 
                 2nd 
                 70.5 
                 74.0 
                 200.0 
                 3089.8 
               
               
                   
                 Mean 
                 71.3 
                 72.5 
                 200.0 
                 3136.4 
               
               
                   
                 STDER 
                 0.8 
                 1.5 
                 0.0 
                 46.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 71.0 
                 85.0 
                 200.0 
                 3120.8 
               
               
                 Xiidra 50 nM 
                 2nd 
                 73.0 
                 75.0 
                 200.0 
                 3245.0 
               
               
                   
                 Mean 
                 72.0 
                 80.0 
                 200.0 
                 3182.9 
               
               
                   
                 STDER 
                 1.0 
                 5.0 
                 0.0 
                 62.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 63.0 
                 80.0 
                 200.0 
                 2621.2 
               
               
                 Xiidra 5 nM 
                 2nd 
                 66.0 
                 77.0 
                 200.0 
                 2809.1 
               
               
                   
                 Mean 
                 64.5 
                 78.5 
                 200.0 
                 2715.2 
               
               
                   
                 STDER 
                 1.5 
                 1.5 
                 0.0 
                 94.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 59.0 
                 86.0 
                 200.0 
                 2369.4 
               
               
                 Xiidra 0.5 nM 
                 2nd 
                 66.5 
                 100.0 
                 200.0 
                 2840.4 
               
               
                   
                 Mean 
                 62.8 
                 93.0 
                 200.0 
                 2604.9 
               
               
                   
                 STDER 
                 3.8 
                 7.0 
                 0.0 
                 235.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 56.0 
                 79.0 
                 200.0 
                 2179.5 
               
               
                 Xiidra 0.05 nM 
                 2nd 
                 64.0 
                 84.0 
                 200.0 
                 2683.9 
               
               
                   
                 Mean 
                 60.0 
                 81.5 
                 200.0 
                 2431.7 
               
               
                   
                 STDER 
                 4.0 
                 2.5 
                 0.0 
                 252.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 61.0 
                 82.0 
                 200.0 
                 2495.5 
               
               
                 Xiidra 0.005 nM 
                 2nd 
                 57.0 
                 66.0 
                 200.0 
                 2242.9 
               
               
                   
                 Mean 
                 59.0 
                 74.0 
                 200.0 
                 2369.2 
               
               
                   
                 STDER 
                 2.0 
                 8.0 
                 0.0 
                 126.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 56.0 
                 77.0 
                 200.0 
                 2179.5 
               
               
                 YY101 500 nM 
                 2nd 
                 53.0 
                 89.0 
                 200.0 
                 1988.8 
               
               
                   
                 3rd 
                 50.0 
                 88.0 
                 200.0 
                 1797.0 
               
               
                   
                 Mean 
                 53.0 
                 84.7 
                 200.0 
                 1988.4 
               
               
                   
                 STDER 
                 1.7 
                 3.8 
                 0.0 
                 110.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 49.0 
                 85.0 
                 200.0 
                 1732.8 
               
               
                 YY101 50 nM 
                 2nd 
                 48.0 
                 83.0 
                 200.0 
                 1668.5 
               
               
                   
                 3rd 
                 53.0 
                 83.0 
                 200.0 
                 1988.8 
               
               
                   
                 Mean 
                 50.0 
                 83.7 
                 200.0 
                 1796.7 
               
               
                   
                 STDER 
                 1.5 
                 0.7 
                 0.0 
                 97.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 53.0 
                 69.0 
                 200.0 
                 1988.8 
               
               
                 YY101 5 nM 
                 2nd 
                 52.0 
                 83.0 
                 200.0 
                 1925.0 
               
               
                   
                 3rd 
                 57.0 
                 60.0 
                 200.0 
                 2242.9 
               
               
                   
                 Mean 
                 54.0 
                 70.7 
                 200.0 
                 2052.2 
               
               
                   
                 STDER 
                 1.5 
                 6.7 
                 0.0 
                 97.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 60.0 
                 72.0 
                 200.0 
                 2432.5 
               
               
                 YY101 0.5 nM 
                 2nd 
                 57.0 
                 93.0 
                 200.0 
                 2242.9 
               
               
                   
                 3rd 
                 55.0 
                 73.0 
                 200.0 
                 2116.1 
               
               
                   
                 Mean 
                 57.3 
                 79.3 
                 200.0 
                 2263.8 
               
               
                   
                 STDER 
                 1.5 
                 6.8 
                 0.0 
                 91.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 50.0 
                 78.0 
                 200.0 
                 1797.0 
               
               
                 YY101 0.05 nM 
                 2nd 
                 63.0 
                 87.0 
                 200.0 
                 2621.2 
               
               
                   
                 3rd 
                 57.0 
                 71.0 
                 200.0 
                 2242.9 
               
               
                   
                 Mean 
                 56.7 
                 78.7 
                 200.0 
                 2220.4 
               
               
                   
                 STDER 
                 3.8 
                 4.6 
                 0.0 
                 238.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 67.0 
                 81.0 
                 200.0 
                 2871.6 
               
               
                 YY101 0.005 nM 
                 2nd 
                 59.0 
                 79.0 
                 200.0 
                 2369.4 
               
               
                   
                 3rd 
                 56.0 
                 67.0 
                 200.0 
                 2179.5 
               
               
                   
                 Mean 
                 60.7 
                 75.7 
                 200.0 
                 2473.5 
               
               
                   
                 STDER 
                 3.3 
                 4.4 
                 0.0 
                 206.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 55.0 
                 85.0 
                 200.0 
                 2116.1 
               
               
                 YDE011 500 nM 
                 2nd 
                 32.5 
                 84.0 
                 200.0 
                 647.8 
               
               
                   
                 3rd 
                 41.5 
                 80.0 
                 200.0 
                 1246.7 
               
               
                   
                 Mean 
                 43.0 
                 83.0 
                 200.0 
                 1336.9 
               
               
                   
                 STDER 
                 6.5 
                 1.5 
                 0.0 
                 426.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 49.0 
                 91.0 
                 200.0 
                 1732.8 
               
               
                 YDE011 50 nM 
                 2nd 
                 56.0 
                 77.0 
                 200.0 
                 2179.5 
               
               
                   
                 3rd 
                 29.5 
                 82.0 
                 200.0 
                 442.1 
               
               
                   
                 Mean 
                 44.8 
                 83.3 
                 200.0 
                 1451.5 
               
               
                   
                 STDER 
                 7.9 
                 4.1 
                 0.0 
                 520.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 34.0 
                 95.0 
                 200.0 
                 749.3 
               
               
                 YDE011 5 nM 
                 2nd 
                 44.0 
                 87.0 
                 200.0 
                 1409.7 
               
               
                   
                 3rd 
                 48.0 
                 82.0 
                 200.0 
                 1668.5 
               
               
                   
                 Mean 
                 42.0 
                 88.0 
                 200.0 
                 1275.8 
               
               
                   
                 STDER 
                 4.2 
                 3.8 
                 0.0 
                 273.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 43.0 
                 90.0 
                 200.0 
                 1344.6 
               
               
                 YDE011 0.5 nM 
                 2nd 
                 53.0 
                 83.0 
                 200.0 
                 1988.8 
               
               
                   
                 3rd 
                 50.0 
                 81.0 
                 200.0 
                 1797.0 
               
               
                   
                 Mean 
                 48.7 
                 84.7 
                 200.0 
                 1710.1 
               
               
                   
                 STDER 
                 3.0 
                 2.7 
                 0.0 
                 191.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 51.0 
                 83.0 
                 200.0 
                 1861.0 
               
               
                 YDE011 0.05 nM 
                 2nd 
                 54.0 
                 85.0 
                 200.0 
                 2052.5 
               
               
                   
                 3rd 
                 54.0 
                 87.0 
                 200.0 
                 2052.5 
               
               
                   
                 Mean 
                 53.0 
                 85.0 
                 200.0 
                 1988.7 
               
               
                   
                 STDER 
                 1.0 
                 1.2 
                 0.0 
                 63.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 55.0 
                 74.0 
                 200.0 
                 2116.1 
               
               
                 YDE011 0.005 nM 
                 2nd 
                 53.0 
                 71.0 
                 200.0 
                 1988.8 
               
               
                   
                 3rd 
                 51.0 
                 98.0 
                 200.0 
                 1861.0 
               
               
                   
                 Mean 
                 53.0 
                 81.0 
                 200.0 
                 1988.6 
               
               
                   
                 STDER 
                 1.2 
                 8.5 
                 0.0 
                 73.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 47.0 
                 77.0 
                 200.0 
                 1604.0 
               
               
                 YDE043 500 nM 
                 2nd 
                 39.5 
                 82.0 
                 200.0 
                 1115.3 
               
               
                   
                 3rd 
                 43.0 
                 80.0 
                 200.0 
                 1344.6 
               
               
                   
                 Mean 
                 43.2 
                 79.7 
                 200.0 
                 1354.6 
               
               
                   
                 STDER 
                 2.2 
                 1.5 
                 0.0 
                 141.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 53.5 
                 76.0 
                 200.0 
                 2020.6 
               
               
                 YDE043 50 nM 
                 2nd 
                 50.0 
                 93.0 
                 200.0 
                 1797.0 
               
               
                   
                 3rd 
                 45.0 
                 88.0 
                 200.0 
                 1474.6 
               
               
                   
                 Mean 
                 49.5 
                 85.7 
                 200.0 
                 1764.1 
               
               
                   
                 STDER 
                 2.5 
                 5.0 
                 0.0 
                 158.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 44.5 
                 92.0 
                 200.0 
                 1442.2 
               
               
                 YDE043 5 nM 
                 2nd 
                 54.0 
                 74.0 
                 200.0 
                 2052.5 
               
               
                   
                 3rd 
                 45.0 
                 120.0 
                 200.0 
                 1474.6 
               
               
                   
                 Mean 
                 47.8 
                 95.3 
                 200.0 
                 1656.4 
               
               
                   
                 STDER 
                 3.1 
                 13.4 
                 0.0 
                 198.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 49.5 
                 54.0 
                 200.0 
                 1764.9 
               
               
                 YDE043 0.5 nM 
                 2nd 
                 44.0 
                 85.0 
                 200.0 
                 1409.7 
               
               
                   
                 3rd 
                 44.0 
                 73.0 
                 200.0 
                 1409.7 
               
               
                   
                 Mean 
                 45.8 
                 70.7 
                 200.0 
                 1528.1 
               
               
                   
                 STDER 
                 1.8 
                 9.0 
                 0.0 
                 118.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 40.5 
                 82.0 
                 200.0 
                 1181.1 
               
               
                 YDE043 0.05 nM 
                 2nd 
                 46.0 
                 79.0 
                 200.0 
                 1539.4 
               
               
                   
                 3rd 
                 45.0 
                 80.0 
                 200.0 
                 1474.6 
               
               
                   
                 Mean 
                 43.8 
                 80.3 
                 200.0 
                 1398.4 
               
               
                   
                 STDER 
                 1.7 
                 0.9 
                 0.0 
                 110.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 54.0 
                 80.0 
                 200.0 
                 2052.5 
               
               
                 YDE043 0.005 nM 
                 2nd 
                 50.0 
                 81.0 
                 200.0 
                 1797.0 
               
               
                   
                 3rd 
                 58.0 
                 95.0 
                 200.0 
                 2306.2 
               
               
                   
                 Mean 
                 54.0 
                 85.3 
                 200.0 
                 2051.9 
               
               
                   
                 STDER 
                 2.3 
                 4.8 
                 0.0 
                 147.0 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Bead name: MMP-3 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concen- 
               
               
                   
                   
                   
                   
                 Dilu- 
                 tration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 tion 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 48.0 
                 55.0 
                 1.0 
                 29.2 
               
               
                   
                 2nd 
                 48.0 
                 65.0 
                 1.0 
                 29.2 
               
               
                   
                 Mean 
                 48.0 
                 60.0 
                 1.0 
                 29.2 
               
               
                   
                 STDER 
                 0.0 
                 5.0 
                 0.0 
                 0.0 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 51.0 
                 64.0 
                 200.0 
                 6062.8 
               
               
                   
                 2nd 
                 47.0 
                 67.0 
                 200.0 
                 5771.5 
               
               
                   
                 3rd 
                 49.5 
                 58.0 
                 200.0 
                 5953.3 
               
               
                   
                 Mean 
                 49.2 
                 63.0 
                 200.0 
                 5929.2 
               
               
                   
                 STDER 
                 1.2 
                 2.6 
                 0.0 
                 84.9 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 96.5 
                 50.0 
                 200.0 
                 9511.1 
               
               
                   
                 2nd 
                 87.0 
                 61.0 
                 200.0 
                 8772.5 
               
               
                   
                 3rd 
                 84.0 
                 53.0 
                 200.0 
                 8541.1 
               
               
                   
                 Mean 
                 89.2 
                 54.7 
                 200.0 
                 8941.6 
               
               
                   
                 STDER 
                 3.8 
                 3.3 
                 0.0 
                 292.5 
               
               
                 PBMC + YY101 500 nM 
                 1st 
                 54.0 
                 82.0 
                 200.0 
                 6282.7 
               
               
                   
                 2nd 
                 56.0 
                 81.0 
                 200.0 
                 6429.9 
               
               
                   
                 Mean 
                 55.0 
                 81.5 
                 200.0 
                 6356.3 
               
               
                   
                 STDER 
                 1.0 
                 0.5 
                 0.0 
                 73.6 
               
               
                 PBMC + YY101 50 nM 
                 1st 
                 54.0 
                 66.0 
                 200.0 
                 6282.7 
               
               
                   
                 2nd 
                 49.5 
                 50.0 
                 200.0 
                 5953.3 
               
               
                   
                 Mean 
                 51.8 
                 58.0 
                 200.0 
                 6118.0 
               
               
                   
                 STDER 
                 2.3 
                 8.0 
                 0.0 
                 164.7 
               
               
                 PBMC + YY101 5 nM 
                 1st 
                 52.5 
                 64.0 
                 200.0 
                 6172.6 
               
               
                   
                 2nd 
                 54.5 
                 50.0 
                 200.0 
                 6319.4 
               
               
                   
                 Mean 
                 53.5 
                 57.0 
                 200.0 
                 6246.0 
               
               
                   
                 STDER 
                 1.0 
                 7.0 
                 0.0 
                 73.4 
               
               
                 PBMC + YY101 0.5 nM 
                 1st 
                 51.0 
                 68.0 
                 200.0 
                 6062.8 
               
               
                   
                 2nd 
                 56.0 
                 59.0 
                 200.0 
                 6429.9 
               
               
                   
                 Mean 
                 53.5 
                 63.5 
                 200.0 
                 6246.3 
               
               
                   
                 STDER 
                 2.5 
                 4.5 
                 0.0 
                 183.5 
               
               
                 PBMC + YY101 0.05 
                 1st 
                 57.0 
                 53.0 
                 200.0 
                 6503.7 
               
               
                 uM 
                 2nd 
                 53.5 
                 64.0 
                 200.0 
                 6245.9 
               
               
                   
                 Mean 
                 55.3 
                 58.5 
                 200.0 
                 6374.8 
               
               
                   
                 STDER 
                 1.8 
                 5.5 
                 0.0 
                 128.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 93.0 
                 55.0 
                 200.0 
                 9237.9 
               
               
                 Xiidra 500 nM 
                 2nd 
                 87.5 
                 56.0 
                 200.0 
                 8811.1 
               
               
                   
                 Mean 
                 90.3 
                 55.5 
                 200.0 
                 9024.5 
               
               
                   
                 STDER 
                 2.8 
                 0.5 
                 0.0 
                 213.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 93.0 
                 54.0 
                 200.0 
                 9237.9 
               
               
                 Xiidra 50 nM 
                 2nd 
                 99.0 
                 61.0 
                 200.0 
                 9707.0 
               
               
                   
                 Mean 
                 96.0 
                 57.5 
                 200.0 
                 9472.4 
               
               
                   
                 STDER 
                 3.0 
                 3.5 
                 0.0 
                 234.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 90.0 
                 73.0 
                 200.0 
                 9004.8 
               
               
                 Xiidra 5 nM 
                 2nd 
                 97.0 
                 67.0 
                 200.0 
                 9550.2 
               
               
                   
                 Mean 
                 93.5 
                 70.0 
                 200.0 
                 9277.5 
               
               
                   
                 STDER 
                 3.5 
                 3.0 
                 0.0 
                 272.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 79.0 
                 84.0 
                 200.0 
                 8157.7 
               
               
                 Xiidra 0.5 nM 
                 2nd 
                 87.0 
                 63.0 
                 200.0 
                 8772.5 
               
               
                   
                 Mean 
                 83.0 
                 73.5 
                 200.0 
                 8465.1 
               
               
                   
                 STDER 
                 4.0 
                 10.5 
                 0.0 
                 307.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 81.0 
                 70.0 
                 200.0 
                 8310.7 
               
               
                 Xiidra 0.05 nM 
                 2nd 
                 85.0 
                 73.0 
                 200.0 
                 8618.2 
               
               
                   
                 Mean 
                 83.0 
                 71.5 
                 200.0 
                 8464.4 
               
               
                   
                 STDER 
                 2.0 
                 1.5 
                 0.0 
                 153.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 81.0 
                 53.0 
                 200.0 
                 8310.7 
               
               
                 Xiidra 0.005 nM 
                 2nd 
                 83.5 
                 64.0 
                 200.0 
                 8502.7 
               
               
                   
                 Mean 
                 82.3 
                 58.5 
                 200.0 
                 8406.7 
               
               
                   
                 STDER 
                 1.3 
                 5.5 
                 0.0 
                 96.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 77.5 
                 60.0 
                 200.0 
                 8043.1 
               
               
                 YY101 500 nM 
                 2nd 
                 72.5 
                 68.0 
                 200.0 
                 7663.2 
               
               
                   
                 3rd 
                 71.0 
                 62.0 
                 200.0 
                 7549.7 
               
               
                   
                 Mean 
                 73.7 
                 63.3 
                 200.0 
                 7752.0 
               
               
                   
                 STDER 
                 2.0 
                 2.4 
                 0.0 
                 149.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 71.0 
                 53.0 
                 200.0 
                 7549.7 
               
               
                 YY101 50 nM 
                 2nd 
                 73.0 
                 61.0 
                 200.0 
                 7701.1 
               
               
                   
                 3rd 
                 78.0 
                 57.0 
                 200.0 
                 8081.3 
               
               
                   
                 Mean 
                 74.0 
                 57.0 
                 200.0 
                 7777.4 
               
               
                   
                 STDER 
                 2.1 
                 2.3 
                 0.0 
                 158.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 71.0 
                 78.0 
                 200.0 
                 7549.7 
               
               
                 YY101 5 nM 
                 2nd 
                 71.0 
                 54.0 
                 200.0 
                 7549.7 
               
               
                   
                 3rd 
                 85.0 
                 52.0 
                 200.0 
                 8618.2 
               
               
                   
                 Mean 
                 75.7 
                 61.3 
                 200.0 
                 7905.9 
               
               
                   
                 STDER 
                 4.7 
                 8.4 
                 0.0 
                 356.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 73.0 
                 87.0 
                 200.0 
                 7701.1 
               
               
                 YY101 0.5 nM 
                 2nd 
                 74.0 
                 60.0 
                 200.0 
                 7776.9 
               
               
                   
                 3rd 
                 74.0 
                 53.0 
                 200.0 
                 7776.9 
               
               
                   
                 Mean 
                 73.7 
                 66.7 
                 200.0 
                 7751.6 
               
               
                   
                 STDER 
                 0.3 
                 10.4 
                 0.0 
                 25.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 74.0 
                 53.0 
                 200.0 
                 7776.9 
               
               
                 YY101 0.05 nM 
                 2nd 
                 84.0 
                 51.0 
                 200.0 
                 8541.1 
               
               
                   
                 3rd 
                 76.0 
                 67.0 
                 200.0 
                 7928.9 
               
               
                   
                 Mean 
                 78.0 
                 57.0 
                 200.0 
                 8082.3 
               
               
                   
                 STDER 
                 3.1 
                 5.0 
                 0.0 
                 233.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 71.0 
                 51.0 
                 200.0 
                 7549.7 
               
               
                 YY101 0.005 nM 
                 2nd 
                 70.0 
                 60.0 
                 200.0 
                 7474.3 
               
               
                   
                 3rd 
                 72.0 
                 69.0 
                 200.0 
                 7625.3 
               
               
                   
                 Mean 
                 71.0 
                 60.0 
                 200.0 
                 7549.8 
               
               
                   
                 STDER 
                 0.6 
                 5.2 
                 0.0 
                 43.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 74.0 
                 50.0 
                 200.0 
                 7776.9 
               
               
                 YDE011 500 nM 
                 2nd 
                 41.5 
                 50.0 
                 200.0 
                 5374.6 
               
               
                   
                 3rd 
                 60.0 
                 55.0 
                 200.0 
                 6725.8 
               
               
                   
                 Mean 
                 58.5 
                 51.7 
                 200.0 
                 6625.8 
               
               
                   
                 STDER 
                 9.4 
                 1.7 
                 0.0 
                 695.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 67.0 
                 63.0 
                 200.0 
                 7248.5 
               
               
                 YDE011 50 nM 
                 2nd 
                 70.0 
                 53.0 
                 200.0 
                 7474.3 
               
               
                   
                 3rd 
                 43.0 
                 63.0 
                 200.0 
                 5482.5 
               
               
                   
                 Mean 
                 60.0 
                 59.7 
                 200.0 
                 6735.1 
               
               
                   
                 STDER 
                 8.5 
                 3.3 
                 0.0 
                 629.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 54.0 
                 50.0 
                 200.0 
                 6282.7 
               
               
                 YDE011 5 nM 
                 2nd 
                 61.0 
                 58.0 
                 200.0 
                 6800.1 
               
               
                   
                 3rd 
                 68.0 
                 63.0 
                 200.0 
                 7323.6 
               
               
                   
                 Mean 
                 61.0 
                 57.0 
                 200.0 
                 6802.1 
               
               
                   
                 STDER 
                 4.0 
                 3.8 
                 0.0 
                 300.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 67.0 
                 87.0 
                 200.0 
                 7248.5 
               
               
                 YDE011 0.5 nM 
                 2nd 
                 65.0 
                 50.0 
                 200.0 
                 7098.6 
               
               
                   
                 3rd 
                 64.5 
                 70.0 
                 200.0 
                 7061.2 
               
               
                   
                 Mean 
                 65.5 
                 69.0 
                 200.0 
                 7136.1 
               
               
                   
                 STDER 
                 0.8 
                 10.7 
                 0.0 
                 57.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 70.0 
                 67.0 
                 200.0 
                 7474.3 
               
               
                 YDE011 0.05 nM 
                 2nd 
                 76.0 
                 57.0 
                 200.0 
                 7928.9 
               
               
                   
                 3rd 
                 70.5 
                 50.0 
                 200.0 
                 7512.0 
               
               
                   
                 Mean 
                 72.2 
                 58.0 
                 200.0 
                 7638.4 
               
               
                   
                 STDER 
                 1.9 
                 4.9 
                 0.0 
                 145.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 70.5 
                 66.0 
                 200.0 
                 7512.0 
               
               
                 YDE011 0.005 nM 
                 2nd 
                 68.0 
                 67.0 
                 200.0 
                 7323.6 
               
               
                   
                 3rd 
                 66.0 
                 63.0 
                 200.0 
                 7173.5 
               
               
                   
                 Mean 
                 68.2 
                 65.3 
                 200.0 
                 7336.4 
               
               
                   
                 STDER 
                 1.3 
                 1.2 
                 0.0 
                 97.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 65.0 
                 57.0 
                 200.0 
                 7098.6 
               
               
                 YDE043 500 nM 
                 2nd 
                 72.5 
                 56.0 
                 200.0 
                 7663.2 
               
               
                   
                 3rd 
                 66.0 
                 71.0 
                 200.0 
                 7173.5 
               
               
                   
                 Mean 
                 67.8 
                 61.3 
                 200.0 
                 7311.7 
               
               
                   
                 STDER 
                 2.4 
                 4.8 
                 0.0 
                 177.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 73.0 
                 53.0 
                 200.0 
                 7701.1 
               
               
                 YDE043 50 nM 
                 2nd 
                 69.0 
                 51.0 
                 200.0 
                 7398.9 
               
               
                   
                 3rd 
                 62.5 
                 50.0 
                 200.0 
                 6911.8 
               
               
                   
                 Mean 
                 68.2 
                 51.3 
                 200.0 
                 7337.3 
               
               
                   
                 STDER 
                 3.1 
                 0.9 
                 0.0 
                 229.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 70.0 
                 55.0 
                 200.0 
                 7474.3 
               
               
                 YDE043 5 nM 
                 2nd 
                 70.0 
                 61.0 
                 200.0 
                 7474.3 
               
               
                   
                 3rd 
                 68.0 
                 75.0 
                 200.0 
                 7323.6 
               
               
                   
                 Mean 
                 69.3 
                 63.7 
                 200.0 
                 7424.0 
               
               
                   
                 STDER 
                 0.7 
                 5.9 
                 0.0 
                 50.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 65.0 
                 58.0 
                 200.0 
                 7098.6 
               
               
                 YDE043 0.5 nM 
                 2nd 
                 67.0 
                 69.0 
                 200.0 
                 7248.5 
               
               
                   
                 3rd 
                 66.0 
                 65.0 
                 200.0 
                 7173.5 
               
               
                   
                 Mean 
                 66.0 
                 64.0 
                 200.0 
                 7173.5 
               
               
                   
                 STDER 
                 0.6 
                 3.2 
                 0.0 
                 43.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 64.5 
                 60.0 
                 200.0 
                 7061.2 
               
               
                 YDE043 0.05 nM 
                 2nd 
                 67.0 
                 50.0 
                 200.0 
                 7248.5 
               
               
                   
                 3rd 
                 65.0 
                 73.0 
                 200.0 
                 7098.6 
               
               
                   
                 Mean 
                 65.5 
                 61.0 
                 200.0 
                 7136.1 
               
               
                   
                 STDER 
                 0.8 
                 6.7 
                 0.0 
                 57.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 66.0 
                 55.0 
                 200.0 
                 7173.5 
               
               
                 YDE043 0.005 nM 
                 2nd 
                 74.0 
                 50.0 
                 200.0 
                 7776.9 
               
               
                   
                 3rd 
                 78.0 
                 61.0 
                 200.0 
                 8081.3 
               
               
                   
                 Mean 
                 72.7 
                 55.3 
                 200.0 
                 7677.2 
               
               
                   
                 STDER 
                 3.5 
                 3.2 
                 0.0 
                 266.8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 15 
               
             
            
               
                   
               
               
                 Bead name: CCL2/MCP-1 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concentration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 Dilution 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 18.0 
                 79.0 
                 1.0 
                 21.6 
               
               
                   
                 2nd 
                 19.0 
                 96.0 
                 1.0 
                 22.1 
               
               
                   
                 Mean 
                 18.5 
                 87.5 
                 1.0 
                 21.9 
               
               
                   
                 STDER 
                 0.5 
                 8.5 
                 0.0 
                 0.3 
               
               
                 PBMC + 
                 1st 
                 17.0 
                 73.0 
                 200.0 
                 4216.3 
               
               
                 0.5% DMSO 
                 2nd 
                 18.0 
                 81.0 
                 200.0 
                 4321.5 
               
               
                   
                 3rd 
                 18.0 
                 107.0 
                 200.0 
                 4321.5 
               
               
                   
                 Mean 
                 17.7 
                 87.0 
                 200.0 
                 4286.5 
               
               
                   
                 STDER 
                 0.3 
                 10.3 
                 0.0 
                 35.1 
               
               
                 PBMC + 
                 1st 
                 36.0 
                 78.0 
                 200.0 
                 6053.2 
               
               
                 LPS(5 ug/ml) 
                 2nd 
                 31.0 
                 86.0 
                 200.0 
                 5597.9 
               
               
                   
                 3rd 
                 28.0 
                 93.0 
                 200.0 
                 5316.3 
               
               
                   
                 Mean 
                 31.7 
                 85.7 
                 200.0 
                 5655.8 
               
               
                   
                 STDER 
                 2.3 
                 4.3 
                 0.0 
                 214.7 
               
               
                 PBMC + 
                 1st 
                 18.0 
                 83.0 
                 200.0 
                 4321.5 
               
               
                 YY101 500 nM 
                 2nd 
                 21.0 
                 111.0 
                 200.0 
                 4630.2 
               
               
                   
                 Mean 
                 19.5 
                 97.0 
                 200.0 
                 4475.9 
               
               
                   
                 STDER 
                 1.5 
                 14.0 
                 0.0 
                 154.4 
               
               
                 PBMC + 
                 1st 
                 18.0 
                 86.0 
                 200.0 
                 4321.5 
               
               
                 YY101 50 nM 
                 2nd 
                 19.0 
                 87.0 
                 200.0 
                 4425.5 
               
               
                   
                 Mean 
                 18.5 
                 86.5 
                 200.0 
                 4373.5 
               
               
                   
                 STDER 
                 0.5 
                 0.5 
                 0.0 
                 52.0 
               
               
                 PBMC + 
                 1st 
                 19.0 
                 106.0 
                 200.0 
                 4425.5 
               
               
                 YY101 5 nM 
                 2nd 
                 19.0 
                 93.0 
                 200.0 
                 4425.5 
               
               
                   
                 Mean 
                 19.0 
                 99.5 
                 200.0 
                 4425.5 
               
               
                   
                 STDER 
                 0.0 
                 6.5 
                 0.0 
                 0.0 
               
               
                 PBMC + 
                 1st 
                 20.0 
                 78.0 
                 200.0 
                 4528.4 
               
               
                 YY101 0.5 nM 
                 2nd 
                 23.0 
                 90.0 
                 200.0 
                 4830.8 
               
               
                   
                 Mean 
                 21.5 
                 84.0 
                 200.0 
                 4679.6 
               
               
                   
                 STDER 
                 1.5 
                 6.0 
                 0.0 
                 151.2 
               
               
                 PBMC + 
                 1st 
                 33.0 
                 69.0 
                 200.0 
                 5782.0 
               
               
                 YY101 0.05 uM 
                 2nd 
                 17.5 
                 96.0 
                 200.0 
                 4269.1 
               
               
                   
                 Mean 
                 25.3 
                 82.5 
                 200.0 
                 5025.5 
               
               
                   
                 STDER 
                 7.8 
                 13.5 
                 0.0 
                 756.4 
               
               
                 PBMC + 
                 1st 
                 34.0 
                 94.0 
                 200.0 
                 5873.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 32.0 
                 76.0 
                 200.0 
                 5690.3 
               
               
                 Xiidra 500 nM 
                 Mean 
                 33.0 
                 85.0 
                 200.0 
                 5781.6 
               
               
                   
                 STDER 
                 1.0 
                 9.0 
                 0.0 
                 91.4 
               
               
                 PBMC + 
                 1st 
                 35.0 
                 88.0 
                 200.0 
                 5963.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 36.0 
                 101.0 
                 200.0 
                 6053.2 
               
               
                 Xiidra 50 nM 
                 Mean 
                 35.5 
                 94.5 
                 200.0 
                 6008.3 
               
               
                   
                 STDER 
                 0.5 
                 6.5 
                 0.0 
                 44.9 
               
               
                 PBMC + 
                 1st 
                 27.0 
                 90.0 
                 200.0 
                 5220.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 35.5 
                 90.0 
                 200.0 
                 6008.4 
               
               
                 Xiidra 5 nM 
                 Mean 
                 31.3 
                 90.0 
                 200.0 
                 5614.6 
               
               
                   
                 STDER 
                 4.3 
                 0.0 
                 0.0 
                 393.7 
               
               
                 PBMC + 
                 1st 
                 28.0 
                 92.0 
                 200.0 
                 5316.3 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 27.5 
                 90.0 
                 200.0 
                 5268.7 
               
               
                 Xiidra 0.5 nM 
                 Mean 
                 27.8 
                 91.0 
                 200.0 
                 5292.5 
               
               
                   
                 STDER 
                 0.3 
                 1.0 
                 0.0 
                 23.8 
               
               
                 PBMC + 
                 1st 
                 30.0 
                 91.0 
                 200.0 
                 5504.7 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 28.0 
                 86.0 
                 200.0 
                 5316.3 
               
               
                 Xiidra 0.05 nM 
                 Mean 
                 29.0 
                 88.5 
                 200.0 
                 5410.5 
               
               
                   
                 STDER 
                 1.0 
                 2.5 
                 0.0 
                 94.2 
               
               
                 PBMC + 
                 1st 
                 27.0 
                 93.0 
                 200.0 
                 5220.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 28.0 
                 70.0 
                 200.0 
                 5316.3 
               
               
                 Xiidra 0.005 nM 
                 Mean 
                 27.5 
                 81.5 
                 200.0 
                 5268.6 
               
               
                   
                 STDER 
                 0.5 
                 11.5 
                 0.0 
                 47.7 
               
               
                 PBMC + 
                 1st 
                 19.0 
                 69.0 
                 200.0 
                 4425.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 19.0 
                 94.0 
                 200.0 
                 4425.5 
               
               
                 YY101 500 nM 
                 3rd 
                 20.0 
                 107.0 
                 200.0 
                 4528.4 
               
               
                   
                 Mean 
                 19.3 
                 90.0 
                 200.0 
                 4459.8 
               
               
                   
                 STDER 
                 0.3 
                 11.2 
                 0.0 
                 34.3 
               
               
                 PBMC + 
                 1st 
                 20.5 
                 82.0 
                 200.0 
                 4579.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 21.0 
                 81.0 
                 200.0 
                 4630.2 
               
               
                 YY101 50 nM 
                 3rd 
                 21.0 
                 85.0 
                 200.0 
                 4630.2 
               
               
                   
                 Mean 
                 20.8 
                 82.7 
                 200.0 
                 4613.3 
               
               
                   
                 STDER 
                 0.2 
                 1.2 
                 0.0 
                 16.9 
               
               
                 PBMC + 
                 1st 
                 21.0 
                 106.0 
                 200.0 
                 4630.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 20.0 
                 81.0 
                 200.0 
                 4528.4 
               
               
                 YY101 5 nM 
                 3rd 
                 22.0 
                 71.0 
                 200.0 
                 4731.0 
               
               
                   
                 Mean 
                 21.0 
                 86.0 
                 200.0 
                 4629.9 
               
               
                   
                 STDER 
                 0.6 
                 10.4 
                 0.0 
                 58.5 
               
               
                 PBMC + 
                 1st 
                 21.0 
                 98.0 
                 200.0 
                 4630.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 20.0 
                 82.0 
                 200.0 
                 4528.4 
               
               
                 YY101 0.5 nM 
                 3rd 
                 21.0 
                 85.0 
                 200.0 
                 4630.2 
               
               
                   
                 Mean 
                 20.7 
                 88.3 
                 200.0 
                 4596.3 
               
               
                   
                 STDER 
                 0.3 
                 4.9 
                 0.0 
                 33.9 
               
               
                 PBMC + 
                 1st 
                 20.0 
                 87.0 
                 200.0 
                 4528.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 22.0 
                 86.0 
                 200.0 
                 4731.0 
               
               
                 YY101 0.05 nM 
                 3rd 
                 20.0 
                 94.0 
                 200.0 
                 4528.4 
               
               
                   
                 Mean 
                 20.7 
                 89.0 
                 200.0 
                 4596.0 
               
               
                   
                 STDER 
                 0.7 
                 2.5 
                 0.0 
                 67.5 
               
               
                 PBMC + 
                 1st 
                 20.0 
                 89.0 
                 200.0 
                 4528.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 21.0 
                 88.0 
                 200.0 
                 4630.2 
               
               
                 YY101 0.005 nM 
                 3rd 
                 19.0 
                 74.0 
                 200.0 
                 4425.5 
               
               
                   
                 Mean 
                 20.0 
                 83.7 
                 200.0 
                 4528.1 
               
               
                   
                 STDER 
                 0.6 
                 4.8 
                 0.0 
                 59.1 
               
               
                 PBMC + 
                 1st 
                 22.0 
                 89.0 
                 200.0 
                 4731.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 12.0 
                 101.0 
                 200.0 
                 3670.6 
               
               
                 YDE011 500 nM 
                 3rd 
                 16.0 
                 91.0 
                 200.0 
                 4109.9 
               
               
                   
                 Mean 
                 16.7 
                 93.7 
                 200.0 
                 4170.5 
               
               
                   
                 STDER 
                 2.9 
                 3.7 
                 0.0 
                 307.6 
               
               
                 PBMC + 
                 1st 
                 18.0 
                 89.0 
                 200.0 
                 4321.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 20.0 
                 79.0 
                 200.0 
                 4528.4 
               
               
                 YDE011 50 nM 
                 3rd 
                 12.0 
                 74.0 
                 200.0 
                 3670.6 
               
               
                   
                 Mean 
                 16.7 
                 80.7 
                 200.0 
                 4173.5 
               
               
                   
                 STDER 
                 2.4 
                 4.4 
                 0.0 
                 258.5 
               
               
                 PBMC + 
                 1st 
                 14.0 
                 92.0 
                 200.0 
                 3893.1 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 18.0 
                 88.0 
                 200.0 
                 4321.5 
               
               
                 YDE011 5 nM 
                 3rd 
                 18.0 
                 103.0 
                 200.0 
                 4321.5 
               
               
                   
                 Mean 
                 16.7 
                 94.3 
                 200.0 
                 4178.7 
               
               
                   
                 STDER 
                 1.3 
                 4.5 
                 0.0 
                 142.8 
               
               
                 PBMC + 
                 1st 
                 19.0 
                 115.0 
                 200.0 
                 4425.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 17.5 
                 86.0 
                 200.0 
                 4269.1 
               
               
                 YDE011 0.5 nM 
                 3rd 
                 17.0 
                 97.0 
                 200.0 
                 4216.3 
               
               
                   
                 Mean 
                 17.8 
                 99.3 
                 200.0 
                 4303.7 
               
               
                   
                 STDER 
                 0.6 
                 8.5 
                 0.0 
                 62.8 
               
               
                 PBMC + 
                 1st 
                 19.0 
                 95.0 
                 200.0 
                 4425.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 19.0 
                 71.0 
                 200.0 
                 4425.5 
               
               
                 YDE011 0.05 nM 
                 3rd 
                 19.5 
                 94.0 
                 200.0 
                 4477.1 
               
               
                   
                 Mean 
                 19.2 
                 86.7 
                 200.0 
                 4442.7 
               
               
                   
                 STDER 
                 0.2 
                 7.8 
                 0.0 
                 17.2 
               
               
                 PBMC + 
                 1st 
                 19.0 
                 69.0 
                 200.0 
                 4425.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 20.0 
                 92.0 
                 200.0 
                 4528.4 
               
               
                 YDE011 0.005 nM 
                 3rd 
                 18.0 
                 101.0 
                 200.0 
                 4321.5 
               
               
                   
                 Mean 
                 19.0 
                 87.3 
                 200.0 
                 4425.2 
               
               
                   
                 STDER 
                 0.6 
                 9.5 
                 0.0 
                 59.7 
               
               
                 PBMC + 
                 1st 
                 21.0 
                 90.0 
                 200.0 
                 4630.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 21.0 
                 92.0 
                 200.0 
                 4630.2 
               
               
                 YDE043 500 nM 
                 3rd 
                 21.0 
                 88.0 
                 200.0 
                 4630.2 
               
               
                   
                 Mean 
                 21.0 
                 90.0 
                 200.0 
                 4630.2 
               
               
                   
                 STDER 
                 0.0 
                 1.2 
                 0.0 
                 0.0 
               
               
                 PBMC + 
                 1st 
                 20.0 
                 92.0 
                 200.0 
                 4528.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 22.0 
                 90.0 
                 200.0 
                 4731.0 
               
               
                 YDE043 50 nM 
                 3rd 
                 19.5 
                 72.0 
                 200.0 
                 4477.1 
               
               
                   
                 Mean 
                 20.5 
                 84.7 
                 200.0 
                 4578.9 
               
               
                   
                 STDER 
                 0.8 
                 6.4 
                 0.0 
                 77.5 
               
               
                 PBMC + 
                 1st 
                 20.5 
                 74.0 
                 200.0 
                 4579.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 19.5 
                 68.0 
                 200.0 
                 4477.1 
               
               
                 YDE043 5 nM 
                 3rd 
                 21.0 
                 103.0 
                 200.0 
                 4630.2 
               
               
                   
                 Mean 
                 20.3 
                 81.7 
                 200.0 
                 4562.3 
               
               
                   
                 STDER 
                 0.4 
                 10.8 
                 0.0 
                 45.0 
               
               
                 PBMC + 
                 1st 
                 19.0 
                 71.0 
                 200.0 
                 4425.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 18.0 
                 86.0 
                 200.0 
                 4321.5 
               
               
                 YDE043 0.5 nM 
                 3rd 
                 19.0 
                 91.0 
                 200.0 
                 4425.5 
               
               
                   
                 Mean 
                 18.7 
                 82.7 
                 200.0 
                 4390.9 
               
               
                   
                 STDER 
                 0.3 
                 6.0 
                 0.0 
                 34.7 
               
               
                 PBMC + 
                 1st 
                 20.0 
                 95.0 
                 200.0 
                 4528.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 19.5 
                 76.0 
                 200.0 
                 4477.1 
               
               
                 YDE043 0.05 nM 
                 3rd 
                 21.0 
                 93.0 
                 200.0 
                 4630.2 
               
               
                   
                 Mean 
                 20.2 
                 88.0 
                 200.0 
                 4545.3 
               
               
                   
                 STDER 
                 0.4 
                 6.0 
                 0.0 
                 45.0 
               
               
                 PBMC + 
                 1st 
                 19.0 
                 71.0 
                 200.0 
                 4425.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 22.0 
                 78.0 
                 200.0 
                 4731.0 
               
               
                 YDE043 0.005 nM 
                 3rd 
                 22.0 
                 99.0 
                 200.0 
                 4731.0 
               
               
                   
                 Mean 
                 21.0 
                 82.7 
                 200.0 
                 4629.2 
               
               
                   
                 STDER 
                 1.0 
                 8.4 
                 0.0 
                 101.8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 16 
               
             
            
               
                   
               
               
                 Bead name: CCL3/MIP-1 alpha 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concentration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 Dilution 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 17.0 
                 64.0 
                 1.0 
                 70.1 
               
               
                   
                 2nd 
                 16.0 
                 70.0 
                 1.0 
                 65.0 
               
               
                   
                 Mean 
                 16.5 
                 67.0 
                 1.0 
                 67.5 
               
               
                   
                 STDER 
                 0.5 
                 3.0 
                 0.0 
                 2.6 
               
               
                 PBMC + 
                 1st 
                 18.0 
                 67.0 
                 200.0 
                 14981.7 
               
               
                 0.5% DMSO 
                 2nd 
                 18.5 
                 58.0 
                 200.0 
                 15447.9 
               
               
                   
                 3rd 
                 19.5 
                 62.0 
                 200.0 
                 16349.6 
               
               
                   
                 Mean 
                 18.7 
                 62.3 
                 200.0 
                 15593.1 
               
               
                   
                 STDER 
                 0.4 
                 2.6 
                 0.0 
                 401.5 
               
               
                 PBMC + 
                 1st 
                 96.0 
                 55.0 
                 200.0 
                 54195.6 
               
               
                 LPS(5 ug/ml) 
                 2nd 
                 66.0 
                 55.0 
                 200.0 
                 42643.2 
               
               
                   
                 3rd 
                 67.0 
                 59.0 
                 200.0 
                 43065.7 
               
               
                   
                 Mean 
                 76.3 
                 56.3 
                 200.0 
                 46634.8 
               
               
                   
                 STDER 
                 9.8 
                 1.3 
                 0.0 
                 3782.4 
               
               
                 PBMC + 
                 1st 
                 17.0 
                 77.0 
                 200.0 
                 14014.0 
               
               
                 YY101 500 nM 
                 2nd 
                 17.0 
                 87.0 
                 200.0 
                 14014.0 
               
               
                   
                 Mean 
                 17.0 
                 82.0 
                 200.0 
                 14014.0 
               
               
                   
                 STDER 
                 0.0 
                 5.0 
                 0.0 
                 0.0 
               
               
                 PBMC + 
                 1st 
                 16.5 
                 50.0 
                 200.0 
                 13510.6 
               
               
                 YY101 50 nM 
                 2nd 
                 18.0 
                 54.0 
                 200.0 
                 14981.7 
               
               
                   
                 Mean 
                 17.3 
                 52.0 
                 200.0 
                 14246.1 
               
               
                   
                 STDER 
                 0.8 
                 2.0 
                 0.0 
                 735.6 
               
               
                 PBMC + 
                 1st 
                 17.0 
                 54.0 
                 200.0 
                 14014.0 
               
               
                 YY101 5 nM 
                 2nd 
                 17.5 
                 62.0 
                 200.0 
                 14504.0 
               
               
                   
                 Mean 
                 17.3 
                 58.0 
                 200.0 
                 14259.0 
               
               
                   
                 STDER 
                 0.3 
                 4.0 
                 0.0 
                 245.0 
               
               
                 PBMC + 
                 1st 
                 19.0 
                 50.0 
                 200.0 
                 15903.7 
               
               
                 YY101 0.5 nM 
                 2nd 
                 20.0 
                 61.0 
                 200.0 
                 16786.4 
               
               
                   
                 Mean 
                 19.5 
                 55.5 
                 200.0 
                 16345.0 
               
               
                   
                 STDER 
                 0.5 
                 5.5 
                 0.0 
                 441.4 
               
               
                 PBMC + 
                 1st 
                 19.0 
                 58.0 
                 200.0 
                 15903.7 
               
               
                 YY101 0.05 uM 
                 2nd 
                 15.0 
                 50.0 
                 200.0 
                 11904.9 
               
               
                   
                 Mean 
                 17.0 
                 54.0 
                 200.0 
                 13904.3 
               
               
                   
                 STDER 
                 2.0 
                 4.0 
                 0.0 
                 1999.4 
               
               
                 PBMC + 
                 1st 
                 81.0 
                 63.0 
                 200.0 
                 48681.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 77.5 
                 54.0 
                 200.0 
                 47325.4 
               
               
                 Xiidra 500 nM 
                 Mean 
                 79.3 
                 58.5 
                 200.0 
                 48003.4 
               
               
                   
                 STDER 
                 1.8 
                 4.5 
                 0.0 
                 678.0 
               
               
                 PBMC + 
                 1st 
                 77.0 
                 56.0 
                 200.0 
                 47129.3 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 79.5 
                 50.0 
                 200.0 
                 48103.9 
               
               
                 Xiidra 50 nM 
                 Mean 
                 78.3 
                 53.0 
                 200.0 
                 47616.6 
               
               
                   
                 STDER 
                 1.3 
                 3.0 
                 0.0 
                 487.3 
               
               
                 PBMC + 
                 1st 
                 69.0 
                 61.0 
                 200.0 
                 43901.3 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 70.0 
                 50.0 
                 200.0 
                 44314.5 
               
               
                 Xiidra 5 nM 
                 Mean 
                 69.5 
                 55.5 
                 200.0 
                 44107.9 
               
               
                   
                 STDER 
                 0.5 
                 5.5 
                 0.0 
                 206.6 
               
               
                 PBMC + 
                 1st 
                 61.0 
                 65.0 
                 200.0 
                 40481.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 69.5 
                 54.0 
                 200.0 
                 44108.3 
               
               
                 Xiidra 0.5 nM 
                 Mean 
                 65.3 
                 59.5 
                 200.0 
                 42294.6 
               
               
                   
                 STDER 
                 4.3 
                 5.5 
                 0.0 
                 1813.6 
               
               
                 PBMC + 
                 1st 
                 66.5 
                 50.0 
                 200.0 
                 42854.8 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 67.0 
                 59.0 
                 200.0 
                 43065.7 
               
               
                 Xiidra 0.05 nM 
                 Mean 
                 66.8 
                 54.5 
                 200.0 
                 42960.3 
               
               
                   
                 STDER 
                 0.3 
                 4.5 
                 0.0 
                 105.4 
               
               
                 PBMC + 
                 1st 
                 61.5 
                 60.0 
                 200.0 
                 40701.1 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 61.0 
                 71.0 
                 200.0 
                 40481.0 
               
               
                 Xiidra 0.005 nM 
                 Mean 
                 61.3 
                 65.5 
                 200.0 
                 40591.0 
               
               
                   
                 STDER 
                 0.3 
                 5.5 
                 0.0 
                 110.1 
               
               
                 PBMC + 
                 1st 
                 46.5 
                 66.0 
                 200.0 
                 33635.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 49.0 
                 75.0 
                 200.0 
                 34887.9 
               
               
                 YY101 500 nM 
                 3rd 
                 46.0 
                 53.0 
                 200.0 
                 33380.1 
               
               
                   
                 Mean 
                 47.2 
                 64.7 
                 200.0 
                 33967.7 
               
               
                   
                 STDER 
                 0.9 
                 6.4 
                 0.0 
                 466.0 
               
               
                 PBMC + 
                 1st 
                 43.0 
                 69.0 
                 200.0 
                 31818.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 52.0 
                 50.0 
                 200.0 
                 36347.5 
               
               
                 YY101 50 nM 
                 3rd 
                 45.0 
                 67.0 
                 200.0 
                 32865.8 
               
               
                   
                 Mean 
                 46.7 
                 62.0 
                 200.0 
                 33677.2 
               
               
                   
                 STDER 
                 2.7 
                 6.0 
                 0.0 
                 1368.9 
               
               
                 PBMC + 
                 1st 
                 46.0 
                 65.0 
                 200.0 
                 33380.1 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 51.0 
                 61.0 
                 200.0 
                 35866.0 
               
               
                 YY101 5 nM 
                 3rd 
                 48.0 
                 61.0 
                 200.0 
                 34390.9 
               
               
                   
                 Mean 
                 48.3 
                 62.3 
                 200.0 
                 34545.7 
               
               
                   
                 STDER 
                 1.5 
                 1.3 
                 0.0 
                 721.8 
               
               
                 PBMC + 
                 1st 
                 51.5 
                 50.0 
                 200.0 
                 36107.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 46.0 
                 57.0 
                 200.0 
                 33380.1 
               
               
                 YY101 0.5 nM 
                 3rd 
                 50.0 
                 50.0 
                 200.0 
                 35379.6 
               
               
                   
                 Mean 
                 49.2 
                 52.3 
                 200.0 
                 34955.7 
               
               
                   
                 STDER 
                 1.6 
                 2.3 
                 0.0 
                 815.3 
               
               
                 PBMC + 
                 1st 
                 51.0 
                 58.0 
                 200.0 
                 35866.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 54.5 
                 72.0 
                 200.0 
                 37530.4 
               
               
                 YY101 0.05 nM 
                 3rd 
                 51.0 
                 60.0 
                 200.0 
                 35866.0 
               
               
                   
                 Mean 
                 52.2 
                 63.3 
                 200.0 
                 36420.8 
               
               
                   
                 STDER 
                 1.2 
                 4.4 
                 0.0 
                 554.8 
               
               
                 PBMC + 
                 1st 
                 49.5 
                 50.0 
                 200.0 
                 35134.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 46.0 
                 56.0 
                 200.0 
                 33380.1 
               
               
                 YY101 0.005 nM 
                 3rd 
                 45.0 
                 59.0 
                 200.0 
                 32865.8 
               
               
                   
                 Mean 
                 46.8 
                 55.0 
                 200.0 
                 33793.5 
               
               
                   
                 STDER 
                 1.4 
                 2.6 
                 0.0 
                 686.7 
               
               
                 PBMC + 
                 1st 
                 48.0 
                 61.0 
                 200.0 
                 34390.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 23.0 
                 73.0 
                 200.0 
                 19244.9 
               
               
                 YDE011 500 nM 
                 3rd 
                 39.5 
                 50.0 
                 200.0 
                 29918.7 
               
               
                   
                 Mean 
                 36.8 
                 61.3 
                 200.0 
                 27851.5 
               
               
                   
                 STDER 
                 7.3 
                 6.6 
                 0.0 
                 4492.8 
               
               
                 PBMC + 
                 1st 
                 41.5 
                 50.0 
                 200.0 
                 31015.1 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 45.0 
                 57.0 
                 200.0 
                 32865.8 
               
               
                 YDE011 50 nM 
                 3rd 
                 25.5 
                 50.0 
                 200.0 
                 21122.8 
               
               
                   
                 Mean 
                 37.3 
                 52.3 
                 200.0 
                 28334.6 
               
               
                   
                 STDER 
                 6.0 
                 2.3 
                 0.0 
                 3645.3 
               
               
                 PBMC + 
                 1st 
                 32.0 
                 69.0 
                 200.0 
                 25497.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 33.0 
                 56.0 
                 200.0 
                 26119.3 
               
               
                 YDE011 5 nM 
                 3rd 
                 40.5 
                 50.0 
                 200.0 
                 30470.7 
               
               
                   
                 Mean 
                 35.2 
                 58.3 
                 200.0 
                 27362.3 
               
               
                   
                 STDER 
                 2.7 
                 5.6 
                 0.0 
                 1564.5 
               
               
                 PBMC + 
                 1st 
                 42.0 
                 71.0 
                 200.0 
                 31284.6 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 39.0 
                 50.0 
                 200.0 
                 29639.8 
               
               
                 YDE011 0.5 nM 
                 3rd 
                 40.5 
                 58.0 
                 200.0 
                 30470.7 
               
               
                   
                 Mean 
                 40.5 
                 59.7 
                 200.0 
                 30465.1 
               
               
                   
                 STDER 
                 0.9 
                 6.1 
                 0.0 
                 474.8 
               
               
                 PBMC + 
                 1st 
                 48.0 
                 59.0 
                 200.0 
                 34390.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 45.0 
                 50.0 
                 200.0 
                 32865.8 
               
               
                 YDE011 0.05 nM 
                 3rd 
                 50.5 
                 70.0 
                 200.0 
                 35623.4 
               
               
                   
                 Mean 
                 47.8 
                 59.7 
                 200.0 
                 34293.4 
               
               
                   
                 STDER 
                 1.6 
                 5.8 
                 0.0 
                 797.5 
               
               
                 PBMC + 
                 1st 
                 43.0 
                 51.0 
                 200.0 
                 31818.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 37.5 
                 50.0 
                 200.0 
                 28790.7 
               
               
                 YDE011 0.005 nM 
                 3rd 
                 42.0 
                 69.0 
                 200.0 
                 31284.6 
               
               
                   
                 Mean 
                 40.8 
                 56.7 
                 200.0 
                 30631.2 
               
               
                   
                 STDER 
                 1.7 
                 6.2 
                 0.0 
                 933.1 
               
               
                 PBMC + 
                 1st 
                 37.0 
                 50.0 
                 200.0 
                 28503.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 33.5 
                 54.0 
                 200.0 
                 26426.3 
               
               
                 YDE043 500 nM 
                 3rd 
                 38.5 
                 64.0 
                 200.0 
                 29358.9 
               
               
                   
                 Mean 
                 36.3 
                 56.0 
                 200.0 
                 28096.2 
               
               
                   
                 STDER 
                 1.5 
                 4.2 
                 0.0 
                 870.7 
               
               
                 PBMC + 
                 1st 
                 46.0 
                 55.0 
                 200.0 
                 33380.1 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 46.0 
                 58.0 
                 200.0 
                 33380.1 
               
               
                 YDE043 50 nM 
                 3rd 
                 37.0 
                 70.0 
                 200.0 
                 28503.4 
               
               
                   
                 Mean 
                 43.0 
                 61.0 
                 200.0 
                 31754.6 
               
               
                   
                 STDER 
                 3.0 
                 4.6 
                 0.0 
                 1625.6 
               
               
                 PBMC + 
                 1st 
                 43.0 
                 54.0 
                 200.0 
                 31818.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 45.0 
                 59.0 
                 200.0 
                 32865.8 
               
               
                 YDE043 5 nM 
                 3rd 
                 41.0 
                 68.0 
                 200.0 
                 30743.9 
               
               
                   
                 Mean 
                 43.0 
                 60.3 
                 200.0 
                 31809.4 
               
               
                   
                 STDER 
                 1.2 
                 4.1 
                 0.0 
                 612.6 
               
               
                 PBMC + 
                 1st 
                 39.0 
                 53.0 
                 200.0 
                 29639.8 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 42.0 
                 57.0 
                 200.0 
                 31284.6 
               
               
                 YDE043 0.5 nM 
                 3rd 
                 38.0 
                 54.0 
                 200.0 
                 29075.9 
               
               
                   
                 Mean 
                 39.7 
                 54.7 
                 200.0 
                 30000.1 
               
               
                   
                 STDER 
                 1.2 
                 1.2 
                 0.0 
                 662.6 
               
               
                 PBMC + 
                 1st 
                 36.5 
                 56.0 
                 200.0 
                 28213.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 40.0 
                 70.0 
                 200.0 
                 30195.7 
               
               
                 YDE043 0.05 nM 
                 3rd 
                 40.0 
                 52.0 
                 200.0 
                 30195.7 
               
               
                   
                 Mean 
                 38.8 
                 59.3 
                 200.0 
                 29535.1 
               
               
                   
                 STDER 
                 1.2 
                 5.5 
                 0.0 
                 660.6 
               
               
                 PBMC + 
                 1st 
                 44.0 
                 50.0 
                 200.0 
                 32345.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 54.0 
                 63.0 
                 200.0 
                 37296.1 
               
               
                 YDE043 0.005 nM 
                 3rd 
                 74.0 
                 59.0 
                 200.0 
                 45939.1 
               
               
                   
                 Mean 
                 57.3 
                 57.3 
                 200.0 
                 38526.9 
               
               
                   
                 STDER 
                 8.8 
                 3.8 
                 0.0 
                 3972.1 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 17 
               
             
            
               
                   
               
               
                 Bead name: CCL4/MIP-1 beta 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concentration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 Dilution 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 6.0 
                 1.0 
                 65.0 
                 &lt;148.02 
               
               
                   
                 2nd 
                 6.0 
                 1.0 
                 58.0 
                 &lt;148.02 
               
               
                   
                 Mean 
                 6.0 
                 1.0 
                 61.5 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 3.5 
                 #DIV/0! 
               
               
                 PBMC + 
                 1st 
                 6.0 
                 200.0 
                 70.0 
                 &lt;148.02 
               
               
                 0.5% DMSO 
                 2nd 
                 6.0 
                 200.0 
                 70.0 
                 &lt;148.02 
               
               
                   
                 3rd 
                 6.0 
                 200.0 
                 94.0 
                 &lt;148.02 
               
               
                   
                 Mean 
                 6.0 
                 200.0 
                 78.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 8.0 
                 #DIV/0! 
               
               
                 PBMC + 
                 1st 
                 20.0 
                 200.0 
                 73.0 
                 36796.9 
               
               
                 LPS(5 ug/ml) 
                 2nd 
                 17.0 
                 200.0 
                 73.0 
                 31897.6 
               
               
                   
                 3rd 
                 18.0 
                 200.0 
                 104.0 
                 33639.0 
               
               
                   
                 Mean 
                 18.3 
                 200.0 
                 83.3 
                 34111.2 
               
               
                   
                 STDER 
                 0.9 
                 0.0 
                 10.3 
                 1433.9 
               
               
                 PBMC + 
                 1st 
                 6.0 
                 200.0 
                 59.0 
                 &lt;148.02 
               
               
                 YY101 500 nM 
                 2nd 
                 7.0 
                 200.0 
                 67.0 
                 &lt;148.02 
               
               
                   
                 Mean 
                 6.5 
                 200.0 
                 63.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 4.0 
                 #DIV/0! 
               
               
                 PBMC + 
                 1st 
                 8.0 
                 200.0 
                 62.0 
                 &lt;148.02 
               
               
                 YY101 50 nM 
                 2nd 
                 7.0 
                 200.0 
                 110.0 
                 &lt;148.02 
               
               
                   
                 Mean 
                 7.5 
                 200.0 
                 86.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 24.0 
                 #DIV/0! 
               
               
                 PBMC + 
                 1st 
                 7.0 
                 200.0 
                 107.0 
                 &lt;148.02 
               
               
                 YY101 5 nM 
                 2nd 
                 6.0 
                 200.0 
                 95.0 
                 &lt;148.02 
               
               
                   
                 Mean 
                 6.5 
                 200.0 
                 101.0 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 6.0 
                 #DIV/0! 
               
               
                 PBMC + 
                 1st 
                 6.0 
                 200.0 
                 73.0 
                 &lt;148.02 
               
               
                 YY101 0.5 nM 
                 2nd 
                 6.0 
                 200.0 
                 98.0 
                 &lt;148.02 
               
               
                   
                 Mean 
                 6.0 
                 200.0 
                 85.5 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 12.5 
                 #DIV/0! 
               
               
                 PBMC + 
                 1st 
                 6.0 
                 200.0 
                 72.0 
                 &lt;148.02 
               
               
                 YY101 0.05 uM 
                 2nd 
                 5.0 
                 200.0 
                 77.0 
                 &lt;148.02 
               
               
                   
                 Mean 
                 5.5 
                 200.0 
                 74.5 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 2.5 
                 #DIV/0! 
               
               
                 PBMC + 
                 1st 
                 20.0 
                 200.0 
                 66.0 
                 36796.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 18.0 
                 200.0 
                 70.0 
                 33639.0 
               
               
                 Xiidra 500 nM 
                 Mean 
                 19.0 
                 200.0 
                 68.0 
                 35218.0 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 2.0 
                 1578.9 
               
               
                 PBMC + 
                 1st 
                 15.0 
                 200.0 
                 63.0 
                 27945.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 16.0 
                 200.0 
                 68.0 
                 30013.2 
               
               
                 Xiidra 50 nM 
                 Mean 
                 15.5 
                 200.0 
                 65.5 
                 28979.2 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 2.5 
                 1034.0 
               
               
                 PBMC + 
                 1st 
                 16.0 
                 200.0 
                 70.0 
                 30013.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 16.0 
                 200.0 
                 90.0 
                 30013.2 
               
               
                 Xiidra 5 nM 
                 Mean 
                 16.0 
                 200.0 
                 80.0 
                 30013.2 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 10.0 
                 0.0 
               
               
                 PBMC + 
                 1st 
                 16.0 
                 200.0 
                 81.0 
                 30013.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 17.0 
                 200.0 
                 62.0 
                 31897.6 
               
               
                 Xiidra 0.5 nM 
                 Mean 
                 16.5 
                 200.0 
                 71.5 
                 30955.4 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 9.5 
                 942.2 
               
               
                 PBMC + 
                 1st 
                 15.5 
                 200.0 
                 88.0 
                 29005.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 17.0 
                 200.0 
                 97.0 
                 31897.6 
               
               
                 Xiidra 0.05 nM 
                 Mean 
                 16.3 
                 200.0 
                 92.5 
                 30451.5 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 4.5 
                 1446.1 
               
               
                 PBMC + 
                 1st 
                 16.0 
                 200.0 
                 85.0 
                 30013.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 14.0 
                 200.0 
                 62.0 
                 25629.7 
               
               
                 Xiidra 0.005 nM 
                 Mean 
                 15.0 
                 200.0 
                 73.5 
                 27821.4 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 11.5 
                 2191.8 
               
               
                 PBMC + 
                 1st 
                 11.0 
                 200.0 
                 60.0 
                 15257.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 12.0 
                 200.0 
                 71.0 
                 19701.2 
               
               
                 YY101 500 nM 
                 3rd 
                 12.0 
                 200.0 
                 72.0 
                 19701.2 
               
               
                   
                 Mean 
                 11.7 
                 200.0 
                 67.7 
                 18220.0 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 3.8 
                 1481.3 
               
               
                 PBMC + 
                 1st 
                 11.0 
                 200.0 
                 65.0 
                 15257.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 12.0 
                 200.0 
                 104.0 
                 19701.2 
               
               
                 YY101 50 nM 
                 3rd 
                 11.0 
                 200.0 
                 90.0 
                 15257.5 
               
               
                   
                 Mean 
                 11.3 
                 200.0 
                 86.3 
                 16738.7 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 11.4 
                 1481.3 
               
               
                 PBMC + 
                 1st 
                 11.5 
                 200.0 
                 76.0 
                 17703.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 11.0 
                 200.0 
                 92.0 
                 15257.5 
               
               
                 YY101 5 nM 
                 3rd 
                 11.0 
                 200.0 
                 79.0 
                 15257.5 
               
               
                   
                 Mean 
                 11.2 
                 200.0 
                 82.3 
                 16072.8 
               
               
                   
                 STDER 
                 0.2 
                 0.0 
                 4.9 
                 815.3 
               
               
                 PBMC + 
                 1st 
                 11.5 
                 200.0 
                 98.0 
                 17703.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 14.0 
                 200.0 
                 85.0 
                 25629.7 
               
               
                 YY101 0.5 nM 
                 3rd 
                 12.0 
                 200.0 
                 73.0 
                 19701.2 
               
               
                   
                 Mean 
                 12.5 
                 200.0 
                 85.3 
                 21011.4 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 7.2 
                 2380.0 
               
               
                 PBMC + 
                 1st 
                 14.0 
                 200.0 
                 50.0 
                 25629.7 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 11.5 
                 200.0 
                 74.0 
                 17703.4 
               
               
                 YY101 0.05 nM 
                 3rd 
                 12.0 
                 200.0 
                 83.0 
                 19701.2 
               
               
                   
                 Mean 
                 12.5 
                 200.0 
                 69.0 
                 21011.4 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 9.8 
                 2380.0 
               
               
                 PBMC + 
                 1st 
                 14.0 
                 200.0 
                 84.0 
                 25629.7 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 12.0 
                 200.0 
                 70.0 
                 19701.2 
               
               
                 YY101 0.005 nM 
                 3rd 
                 12.0 
                 200.0 
                 70.0 
                 19701.2 
               
               
                   
                 Mean 
                 12.7 
                 200.0 
                 74.7 
                 21677.4 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 4.7 
                 1976.1 
               
               
                 PBMC + 
                 1st 
                 12.0 
                 200.0 
                 61.0 
                 19701.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 10.0 
                 200.0 
                 89.0 
                 1874.9 
               
               
                 YDE011 500 nM 
                 3rd 
                 12.0 
                 200.0 
                 61.0 
                 19701.2 
               
               
                   
                 Mean 
                 11.3 
                 200.0 
                 70.3 
                 13759.1 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 9.3 
                 5942.1 
               
               
                 PBMC + 
                 1st 
                 13.0 
                 200.0 
                 77.0 
                 22956.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 14.0 
                 200.0 
                 102.0 
                 25629.7 
               
               
                 YDE011 50 nM 
                 3rd 
                 13.0 
                 200.0 
                 74.0 
                 22956.0 
               
               
                   
                 Mean 
                 13.3 
                 200.0 
                 84.3 
                 23847.2 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 8.9 
                 891.2 
               
               
                 PBMC + 
                 1st 
                 11.0 
                 200.0 
                 64.0 
                 15257.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 12.0 
                 200.0 
                 95.0 
                 19701.2 
               
               
                 YDE011 5 nM 
                 3rd 
                 11.0 
                 200.0 
                 103.0 
                 15257.5 
               
               
                   
                 Mean 
                 11.3 
                 200.0 
                 87.3 
                 16738.7 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 11.9 
                 1481.3 
               
               
                 PBMC + 
                 1st 
                 13.0 
                 200.0 
                 95.0 
                 22956.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 13.0 
                 200.0 
                 75.0 
                 22956.0 
               
               
                 YDE011 0.5 nM 
                 3rd 
                 13.0 
                 200.0 
                 76.0 
                 22956.0 
               
               
                   
                 Mean 
                 13.0 
                 200.0 
                 82.0 
                 22956.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 6.5 
                 0.0 
               
               
                 PBMC + 
                 1st 
                 13.0 
                 200.0 
                 53.0 
                 22956.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 13.0 
                 200.0 
                 62.0 
                 22956.0 
               
               
                 YDE011 0.05 nM 
                 3rd 
                 13.0 
                 200.0 
                 92.0 
                 22956.0 
               
               
                   
                 Mean 
                 13.0 
                 200.0 
                 69.0 
                 22956.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 11.8 
                 0.0 
               
               
                 PBMC + 
                 1st 
                 12.0 
                 200.0 
                 87.0 
                 19701.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 13.0 
                 200.0 
                 97.0 
                 22956.0 
               
               
                 YDE011 0.005 nM 
                 3rd 
                 12.0 
                 200.0 
                 74.0 
                 19701.2 
               
               
                   
                 Mean 
                 12.3 
                 200.0 
                 86.0 
                 20786.2 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 6.7 
                 1084.9 
               
               
                 PBMC + 
                 1st 
                 14.0 
                 200.0 
                 79.0 
                 25629.7 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 13.0 
                 200.0 
                 65.0 
                 22956.0 
               
               
                 YDE043 500 nM 
                 3rd 
                 12.0 
                 200.0 
                 74.0 
                 19701.2 
               
               
                   
                 Mean 
                 13.0 
                 200.0 
                 72.7 
                 22762.3 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 4.1 
                 1714.1 
               
               
                 PBMC + 
                 1st 
                 13.0 
                 200.0 
                 79.0 
                 22956.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 13.0 
                 200.0 
                 76.0 
                 22956.0 
               
               
                 YDE043 50 nM 
                 3rd 
                 11.5 
                 200.0 
                 96.0 
                 17703.4 
               
               
                   
                 Mean 
                 12.5 
                 200.0 
                 83.7 
                 21205.1 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 6.2 
                 1750.9 
               
               
                 PBMC + 
                 1st 
                 12.0 
                 200.0 
                 77.0 
                 19701.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 13.0 
                 200.0 
                 73.0 
                 22956.0 
               
               
                 YDE043 5 nM 
                 3rd 
                 12.0 
                 200.0 
                 85.0 
                 19701.2 
               
               
                   
                 Mean 
                 12.3 
                 200.0 
                 78.3 
                 20786.2 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 3.5 
                 1084.9 
               
               
                 PBMC + 
                 1st 
                 13.0 
                 200.0 
                 80.0 
                 22956.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 13.0 
                 200.0 
                 74.0 
                 22956.0 
               
               
                 YDE043 0.5 nM 
                 3rd 
                 12.0 
                 200.0 
                 57.0 
                 19701.2 
               
               
                   
                 Mean 
                 12.7 
                 200.0 
                 70.3 
                 21871.1 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 6.9 
                 1084.9 
               
               
                 PBMC + 
                 1st 
                 12.0 
                 200.0 
                 92.0 
                 19701.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 12.0 
                 200.0 
                 96.0 
                 19701.2 
               
               
                 YDE043 0.05 nM 
                 3rd 
                 12.0 
                 200.0 
                 78.0 
                 19701.2 
               
               
                   
                 Mean 
                 12.0 
                 200.0 
                 88.7 
                 19701.2 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 5.5 
                 0.0 
               
               
                 PBMC + 
                 1st 
                 13.0 
                 200.0 
                 69.0 
                 22956.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 16.0 
                 200.0 
                 73.0 
                 30013.2 
               
               
                 YDE043 0.005 nM 
                 3rd 
                 12.0 
                 200.0 
                 78.0 
                 19701.2 
               
               
                   
                 Mean 
                 13.7 
                 200.0 
                 73.3 
                 24223.5 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 2.6 
                 3043.5 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 18 
               
             
            
               
                   
               
               
                 Bead name: IL-1 alpha/IL-1F1 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concentration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 Dilution 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 20.0 
                 57.0 
                 1.0 
                 0.8 
               
               
                   
                 2nd 
                 16.0 
                 66.0 
                 1.0 
                 0.0 
               
               
                   
                 Mean 
                 18.0 
                 61.5 
                 1.0 
                 0.4 
               
               
                   
                 STDER 
                 2.0 
                 4.5 
                 0.0 
                 0.4 
               
               
                 PBMC + 
                 1st 
                 18.0 
                 63.0 
                 200.0 
                 84.2 
               
               
                 0.5% DMSO 
                 2nd 
                 18.5 
                 62.0 
                 200.0 
                 102.5 
               
               
                   
                 3rd 
                 18.0 
                 89.0 
                 200.0 
                 84.2 
               
               
                   
                 Mean 
                 18.2 
                 71.3 
                 200.0 
                 90.3 
               
               
                   
                 STDER 
                 0.2 
                 8.8 
                 0.0 
                 6.1 
               
               
                 PBMC + 
                 1st 
                 39.0 
                 64.0 
                 200.0 
                 740.9 
               
               
                 LPS(5 ug/ml) 
                 2nd 
                 31.0 
                 63.0 
                 200.0 
                 505.6 
               
               
                   
                 3rd 
                 31.0 
                 73.0 
                 200.0 
                 505.6 
               
               
                   
                 Mean 
                 33.7 
                 66.7 
                 200.0 
                 584.0 
               
               
                   
                 STDER 
                 2.7 
                 3.2 
                 0.0 
                 78.4 
               
               
                 PBMC + 
                 1st 
                 18.0 
                 57.0 
                 200.0 
                 84.2 
               
               
                 YY101 500 nM 
                 2nd 
                 18.0 
                 81.0 
                 200.0 
                 84.2 
               
               
                   
                 Mean 
                 18.0 
                 69.0 
                 200.0 
                 84.2 
               
               
                   
                 STDER 
                 0.0 
                 12.0 
                 0.0 
                 0.0 
               
               
                 PBMC + 
                 1st 
                 19.5 
                 64.0 
                 200.0 
                 138.1 
               
               
                 YY101 50 nM 
                 2nd 
                 18.0 
                 53.0 
                 200.0 
                 84.2 
               
               
                   
                 Mean 
                 18.8 
                 58.5 
                 200.0 
                 111.1 
               
               
                   
                 STDER 
                 0.8 
                 5.5 
                 0.0 
                 27.0 
               
               
                 PBMC + 
                 1st 
                 20.0 
                 75.0 
                 200.0 
                 155.5 
               
               
                 YY101 5 nM 
                 2nd 
                 20.0 
                 60.0 
                 200.0 
                 155.5 
               
               
                   
                 Mean 
                 20.0 
                 67.5 
                 200.0 
                 155.5 
               
               
                   
                 STDER 
                 0.0 
                 7.5 
                 0.0 
                 0.0 
               
               
                 PBMC + 
                 1st 
                 20.0 
                 70.0 
                 200.0 
                 155.5 
               
               
                 YY101 0.5 nM 
                 2nd 
                 20.0 
                 66.0 
                 200.0 
                 155.5 
               
               
                   
                 Mean 
                 20.0 
                 68.0 
                 200.0 
                 155.5 
               
               
                   
                 STDER 
                 0.0 
                 2.0 
                 0.0 
                 0.0 
               
               
                 PBMC + 
                 1st 
                 17.0 
                 74.0 
                 200.0 
                 45.8 
               
               
                 YY101 0.05 uM 
                 2nd 
                 19.5 
                 54.0 
                 200.0 
                 138.1 
               
               
                   
                 Mean 
                 18.3 
                 64.0 
                 200.0 
                 92.0 
               
               
                   
                 STDER 
                 1.3 
                 10.0 
                 0.0 
                 46.2 
               
               
                 PBMC + 
                 1st 
                 38.0 
                 73.0 
                 200.0 
                 712.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 37.0 
                 50.0 
                 200.0 
                 683.0 
               
               
                 Xiidra 500 nM 
                 Mean 
                 37.5 
                 61.5 
                 200.0 
                 697.5 
               
               
                   
                 STDER 
                 0.5 
                 11.5 
                 0.0 
                 14.5 
               
               
                 PBMC + 
                 1st 
                 37.5 
                 74.0 
                 200.0 
                 697.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 40.0 
                 82.0 
                 200.0 
                 769.6 
               
               
                 Xiidra 50 nM 
                 Mean 
                 38.8 
                 78.0 
                 200.0 
                 733.6 
               
               
                   
                 STDER 
                 1.3 
                 4.0 
                 0.0 
                 36.1 
               
               
                 PBMC + 
                 1st 
                 32.5 
                 66.0 
                 200.0 
                 550.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 35.5 
                 66.0 
                 200.0 
                 639.2 
               
               
                 Xiidra 5 nM 
                 Mean 
                 34.0 
                 66.0 
                 200.0 
                 594.9 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 0.0 
                 44.3 
               
               
                 PBMC + 
                 1st 
                 28.5 
                 64.0 
                 200.0 
                 429.6 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 32.0 
                 61.0 
                 200.0 
                 535.6 
               
               
                 Xiidra 0.5 nM 
                 Mean 
                 30.3 
                 62.5 
                 200.0 
                 482.6 
               
               
                   
                 STDER 
                 1.8 
                 1.5 
                 0.0 
                 53.0 
               
               
                 PBMC + 
                 1st 
                 29.0 
                 89.0 
                 200.0 
                 444.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 30.0 
                 70.0 
                 200.0 
                 475.3 
               
               
                 Xiidra 0.05 nM 
                 Mean 
                 29.5 
                 79.5 
                 200.0 
                 460.1 
               
               
                   
                 STDER 
                 0.5 
                 9.5 
                 0.0 
                 15.2 
               
               
                 PBMC + 
                 1st 
                 30.0 
                 66.0 
                 200.0 
                 475.3 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 27.0 
                 62.0 
                 200.0 
                 383.2 
               
               
                 Xiidra 0.005 nM 
                 Mean 
                 28.5 
                 64.0 
                 200.0 
                 429.3 
               
               
                   
                 STDER 
                 1.5 
                 2.0 
                 0.0 
                 46.1 
               
               
                 PBMC + 
                 1st 
                 29.5 
                 56.0 
                 200.0 
                 460.1 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 31.0 
                 74.0 
                 200.0 
                 505.6 
               
               
                 YY101 500nM 
                 3rd 
                 27.0 
                 71.0 
                 200.0 
                 383.2 
               
               
                   
                 Mean 
                 29.2 
                 67.0 
                 200.0 
                 449.6 
               
               
                   
                 STDER 
                 1.2 
                 5.6 
                 0.0 
                 35.7 
               
               
                 PBMC + 
                 1st 
                 27.0 
                 69.0 
                 200.0 
                 383.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 27.5 
                 58.0 
                 200.0 
                 398.7 
               
               
                 YY101 50 nM 
                 3rd 
                 31.0 
                 69.0 
                 200.0 
                 505.6 
               
               
                   
                 Mean 
                 28.5 
                 65.3 
                 200.0 
                 429.2 
               
               
                   
                 STDER 
                 1.3 
                 3.7 
                 0.0 
                 38.5 
               
               
                 PBMC + 
                 1st 
                 29.0 
                 64.0 
                 200.0 
                 444.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 30.0 
                 64.0 
                 200.0 
                 475.3 
               
               
                 YY101 5 nM 
                 3rd 
                 32.0 
                 57.0 
                 200.0 
                 535.6 
               
               
                   
                 Mean 
                 30.3 
                 61.7 
                 200.0 
                 485.3 
               
               
                   
                 STDER 
                 0.9 
                 2.3 
                 0.0 
                 26.7 
               
               
                 PBMC + 
                 1st 
                 29.5 
                 72.0 
                 200.0 
                 460.1 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 29.0 
                 80.0 
                 200.0 
                 444.9 
               
               
                 YY101 0.5 nM 
                 3rd 
                 29.5 
                 60.0 
                 200.0 
                 460.1 
               
               
                   
                 Mean 
                 29.3 
                 70.7 
                 200.0 
                 455.0 
               
               
                   
                 STDER 
                 0.2 
                 5.8 
                 0.0 
                 5.1 
               
               
                 PBMC + 
                 1st 
                 28.0 
                 74.0 
                 200.0 
                 414.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 34.5 
                 66.0 
                 200.0 
                 609.8 
               
               
                 YY101 0.05 nM 
                 3rd 
                 28.0 
                 61.0 
                 200.0 
                 414.2 
               
               
                   
                 Mean 
                 30.2 
                 67.0 
                 200.0 
                 479.4 
               
               
                   
                 STDER 
                 2.2 
                 3.8 
                 0.0 
                 65.2 
               
               
                 PBMC + 
                 1st 
                 28.0 
                 58.0 
                 200.0 
                 414.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 27.0 
                 55.0 
                 200.0 
                 383.2 
               
               
                 YY101 0.005 nM 
                 3rd 
                 30.0 
                 61.0 
                 200.0 
                 475.3 
               
               
                   
                 Mean 
                 28.3 
                 58.0 
                 200.0 
                 424.2 
               
               
                   
                 STDER 
                 0.9 
                 1.7 
                 0.0 
                 27.1 
               
               
                 PBMC + 
                 1st 
                 32.0 
                 60.0 
                 200.0 
                 535.6 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 16.0 
                 67.0 
                 200.0 
                 1.9 
               
               
                 YDE011 500 nM 
                 3rd 
                 24.0 
                 56.0 
                 200.0 
                 288.4 
               
               
                   
                 Mean 
                 24.0 
                 61.0 
                 200.0 
                 275.3 
               
               
                   
                 STDER 
                 4.6 
                 3.2 
                 0.0 
                 154.2 
               
               
                 PBMC + 
                 1st 
                 28.5 
                 74.0 
                 200.0 
                 429.6 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 25.0 
                 59.0 
                 200.0 
                 320.3 
               
               
                 YDE011 50 nM 
                 3rd 
                 18.0 
                 63.0 
                 200.0 
                 84.2 
               
               
                   
                 Mean 
                 23.8 
                 65.3 
                 200.0 
                 278.0 
               
               
                   
                 STDER 
                 3.1 
                 4.5 
                 0.0 
                 101.9 
               
               
                 PBMC + 
                 1st 
                 23.0 
                 75.0 
                 200.0 
                 256.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 24.0 
                 74.0 
                 200.0 
                 288.4 
               
               
                 YDE011 5 nM 
                 3rd 
                 28.5 
                 70.0 
                 200.0 
                 429.6 
               
               
                   
                 Mean 
                 25.2 
                 73.0 
                 200.0 
                 324.6 
               
               
                   
                 STDER 
                 1.7 
                 1.5 
                 0.0 
                 53.3 
               
               
                 PBMC + 
                 1st 
                 28.5 
                 84.0 
                 200.0 
                 429.6 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 27.0 
                 86.0 
                 200.0 
                 383.2 
               
               
                 YDE011 0.5 nM 
                 3rd 
                 27.5 
                 70.0 
                 200.0 
                 398.7 
               
               
                   
                 Mean 
                 27.7 
                 80.0 
                 200.0 
                 403.8 
               
               
                   
                 STDER 
                 0.4 
                 5.0 
                 0.0 
                 13.6 
               
               
                 PBMC + 
                 1st 
                 32.0 
                 74.0 
                 200.0 
                 535.6 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 32.5 
                 70.0 
                 200.0 
                 550.5 
               
               
                 YDE011 0.05 nM 
                 3rd 
                 29.0 
                 66.0 
                 200.0 
                 444.9 
               
               
                   
                 Mean 
                 31.2 
                 70.0 
                 200.0 
                 510.3 
               
               
                   
                 STDER 
                 1.1 
                 2.3 
                 0.0 
                 33.0 
               
               
                 PBMC + 
                 1st 
                 34.0 
                 60.0 
                 200.0 
                 595.1 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 29.5 
                 68.0 
                 200.0 
                 460.1 
               
               
                 YDE011 0.005 nM 
                 3rd 
                 30.0 
                 86.0 
                 200.0 
                 475.3 
               
               
                   
                 Mean 
                 31.2 
                 71.3 
                 200.0 
                 510.2 
               
               
                   
                 STDER 
                 1.4 
                 7.7 
                 0.0 
                 42.7 
               
               
                 PBMC + 
                 1st 
                 30.0 
                 67.0 
                 200.0 
                 475.3 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 28.0 
                 55.0 
                 200.0 
                 414.2 
               
               
                 YDE043 500 nM 
                 3rd 
                 27.0 
                 60.0 
                 200.0 
                 383.2 
               
               
                   
                 Mean 
                 28.3 
                 60.7 
                 200.0 
                 424.2 
               
               
                   
                 STDER 
                 0.9 
                 3.5 
                 0.0 
                 27.1 
               
               
                 PBMC + 
                 1st 
                 30.0 
                 76.0 
                 200.0 
                 475.3 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 29.0 
                 62.0 
                 200.0 
                 444.9 
               
               
                 YDE043 50 nM 
                 3rd 
                 27.0 
                 83.0 
                 200.0 
                 383.2 
               
               
                   
                 Mean 
                 28.7 
                 73.7 
                 200.0 
                 434.5 
               
               
                   
                 STDER 
                 0.9 
                 6.2 
                 0.0 
                 27.1 
               
               
                 PBMC + 
                 1st 
                 31.0 
                 64.0 
                 200.0 
                 505.6 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 29.0 
                 54.0 
                 200.0 
                 444.9 
               
               
                 YDE043 5 nM 
                 3rd 
                 29.0 
                 70.0 
                 200.0 
                 444.9 
               
               
                   
                 Mean 
                 29.7 
                 62.7 
                 200.0 
                 465.1 
               
               
                   
                 STDER 
                 0.7 
                 4.7 
                 0.0 
                 20.2 
               
               
                 PBMC + 
                 1st 
                 26.0 
                 57.0 
                 200.0 
                 351.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 26.5 
                 50.0 
                 200.0 
                 367.6 
               
               
                 YDE043 0.5 nM 
                 3rd 
                 25.5 
                 64.0 
                 200.0 
                 336.2 
               
               
                   
                 Mean 
                 26.0 
                 57.0 
                 200.0 
                 351.9 
               
               
                   
                 STDER 
                 0.3 
                 4.0 
                 0.0 
                 9.1 
               
               
                 PBMC + 
                 1st 
                 26.0 
                 74.0 
                 200.0 
                 351.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 25.0 
                 64.0 
                 200.0 
                 320.3 
               
               
                 YDE043 0.05 nM 
                 3rd 
                 25.0 
                 78.0 
                 200.0 
                 320.3 
               
               
                   
                 Mean 
                 25.3 
                 72.0 
                 200.0 
                 330.9 
               
               
                   
                 STDER 
                 0.3 
                 4.2 
                 0.0 
                 10.5 
               
               
                 PBMC + 
                 1st 
                 27.0 
                 71.0 
                 200.0 
                 383.2 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 30.5 
                 50.0 
                 200.0 
                 490.5 
               
               
                 YDE043 0.005 nM 
                 3rd 
                 34.0 
                 60.0 
                 200.0 
                 595.1 
               
               
                   
                 Mean 
                 30.5 
                 60.3 
                 200.0 
                 489.6 
               
               
                   
                 STDER 
                 2.0 
                 6.1 
                 0.0 
                 61.2 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 19 
               
             
            
               
                   
               
               
                 Bead name: IL-1 beta/IL-1F2 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concen- 
               
               
                   
                   
                   
                   
                 Dilu- 
                 tration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 tion 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 14.0 
                 55.0 
                 1.0 
                 2.1 
               
               
                   
                 2nd 
                 13.0 
                 69.0 
                 1.0 
                 1.8 
               
               
                   
                 Mean 
                 13.5 
                 62.0 
                 1.0 
                 2.0 
               
               
                   
                 STDER 
                 0.5 
                 7.0 
                 0.0 
                 0.1 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 12.0 
                 71.0 
                 200.0 
                 304.1 
               
               
                   
                 2nd 
                 12.0 
                 79.0 
                 200.0 
                 304.1 
               
               
                   
                 3rd 
                 14.0 
                 84.0 
                 200.0 
                 420.1 
               
               
                   
                 Mean 
                 12.7 
                 78.0 
                 200.0 
                 342.8 
               
               
                   
                 STDER 
                 0.7 
                 3.8 
                 0.0 
                 38.7 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 141.5 
                 74.0 
                 200.0 
                 7972.5 
               
               
                   
                 2nd 
                 117.0 
                 69.0 
                 200.0 
                 6511.1 
               
               
                   
                 3rd 
                 106.0 
                 72.0 
                 200.0 
                 5855.9 
               
               
                   
                 Mean 
                 121.5 
                 71.7 
                 200.0 
                 6779.8 
               
               
                   
                 STDER 
                 10.5 
                 1.5 
                 0.0 
                 625.6 
               
               
                 PBMC + YY101 500 nM 
                 1st 
                 14.0 
                 100.0 
                 200.0 
                 420.1 
               
               
                   
                 2nd 
                 14.0 
                 101.0 
                 200.0 
                 420.1 
               
               
                   
                 Mean 
                 14.0 
                 100.5 
                 200.0 
                 420.1 
               
               
                   
                 STDER 
                 0.0 
                 0.5 
                 0.0 
                 0.0 
               
               
                 PBMC + YY101 50 nM 
                 1st 
                 13.0 
                 80.0 
                 200.0 
                 362.0 
               
               
                   
                 2nd 
                 13.0 
                 78.0 
                 200.0 
                 362.0 
               
               
                   
                 Mean 
                 13.0 
                 79.0 
                 200.0 
                 362.0 
               
               
                   
                 STDER 
                 0.0 
                 1.0 
                 0.0 
                 0.0 
               
               
                 PBMC + YY101 5 nM 
                 1st 
                 13.0 
                 80.0 
                 200.0 
                 362.0 
               
               
                   
                 2nd 
                 14.0 
                 77.0 
                 200.0 
                 420.1 
               
               
                   
                 Mean 
                 13.5 
                 78.5 
                 200.0 
                 391.1 
               
               
                   
                 STDER 
                 0.5 
                 1.5 
                 0.0 
                 29.0 
               
               
                 PBMC + YY101 0.5 nM 
                 1st 
                 13.0 
                 63.0 
                 200.0 
                 362.0 
               
               
                   
                 2nd 
                 14.0 
                 84.0 
                 200.0 
                 420.1 
               
               
                   
                 Mean 
                 13.5 
                 73.5 
                 200.0 
                 391.1 
               
               
                   
                 STDER 
                 0.5 
                 10.5 
                 0.0 
                 29.0 
               
               
                 PBMC + YY101 0.05 uM 
                 1st 
                 14.0 
                 89.0 
                 200.0 
                 420.1 
               
               
                   
                 2nd 
                 14.0 
                 67.0 
                 200.0 
                 420.1 
               
               
                   
                 Mean 
                 14.0 
                 78.0 
                 200.0 
                 420.1 
               
               
                   
                 STDER 
                 0.0 
                 11.0 
                 0.0 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 144.5 
                 96.0 
                 200.0 
                 8151.7 
               
               
                 Xiidra 500 nM 
                 2nd 
                 149.0 
                 69.0 
                 200.0 
                 8420.4 
               
               
                   
                 Mean 
                 146.8 
                 82.5 
                 200.0 
                 8286.0 
               
               
                   
                 STDER 
                 2.3 
                 13.5 
                 0.0 
                 134.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 141.0 
                 71.0 
                 200.0 
                 7942.7 
               
               
                 Xiidra 50 nM 
                 2nd 
                 152.0 
                 84.0 
                 200.0 
                 8599.7 
               
               
                   
                 Mean 
                 146.5 
                 77.5 
                 200.0 
                 8271.2 
               
               
                   
                 STDER 
                 5.5 
                 6.5 
                 0.0 
                 328.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 125.0 
                 73.0 
                 200.0 
                 6988.0 
               
               
                 Xiidra 5 nM 
                 2nd 
                 142.0 
                 62.0 
                 200.0 
                 8002.4 
               
               
                   
                 Mean 
                 133.5 
                 67.5 
                 200.0 
                 7495.2 
               
               
                   
                 STDER 
                 8.5 
                 5.5 
                 0.0 
                 507.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 108.0 
                 99.0 
                 200.0 
                 5975.0 
               
               
                 Xiidra 0.5 nM 
                 2nd 
                 124.0 
                 78.0 
                 200.0 
                 6928.3 
               
               
                   
                 Mean 
                 116.0 
                 88.5 
                 200.0 
                 6451.7 
               
               
                   
                 STDER 
                 8.0 
                 10.5 
                 0.0 
                 476.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 116.5 
                 82.0 
                 200.0 
                 6481.3 
               
               
                 Xiidra 0.05 nM 
                 2nd 
                 113.0 
                 95.0 
                 200.0 
                 6272.8 
               
               
                   
                 Mean 
                 114.8 
                 88.5 
                 200.0 
                 6377.0 
               
               
                   
                 STDER 
                 1.8 
                 6.5 
                 0.0 
                 104.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 118.0 
                 71.0 
                 200.0 
                 6570.7 
               
               
                 Xiidra 0.005 nM 
                 2nd 
                 104.0 
                 67.0 
                 200.0 
                 5736.8 
               
               
                   
                 Mean 
                 111.0 
                 69.0 
                 200.0 
                 6153.8 
               
               
                   
                 STDER 
                 7.0 
                 2.0 
                 0.0 
                 416.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 96.0 
                 73.0 
                 200.0 
                 5260.9 
               
               
                 YY101 500 nM 
                 2nd 
                 107.0 
                 95.0 
                 200.0 
                 5915.4 
               
               
                   
                 3rd 
                 101.0 
                 81.0 
                 200.0 
                 5558.3 
               
               
                   
                 Mean 
                 101.3 
                 83.0 
                 200.0 
                 5578.2 
               
               
                   
                 STDER 
                 3.2 
                 6.4 
                 0.0 
                 189.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 95.0 
                 76.0 
                 200.0 
                 5201.4 
               
               
                 YY101 50 nM 
                 2nd 
                 95.0 
                 67.0 
                 200.0 
                 5201.4 
               
               
                   
                 3rd 
                 115.5 
                 66.0 
                 200.0 
                 6421.7 
               
               
                   
                 Mean 
                 101.8 
                 69.7 
                 200.0 
                 5608.2 
               
               
                   
                 STDER 
                 6.8 
                 3.2 
                 0.0 
                 406.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 100.0 
                 78.0 
                 200.0 
                 5498.8 
               
               
                 YY101 5 nM 
                 2nd 
                 101.0 
                 63.0 
                 200.0 
                 5558.3 
               
               
                   
                 3rd 
                 118.0 
                 75.0 
                 200.0 
                 6570.7 
               
               
                   
                 Mean 
                 106.3 
                 72.0 
                 200.0 
                 5875.9 
               
               
                   
                 STDER 
                 5.8 
                 4.6 
                 0.0 
                 347.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 104.0 
                 85.0 
                 200.0 
                 5736.8 
               
               
                 YY101 0.5 nM 
                 2nd 
                 108.0 
                 86.0 
                 200.0 
                 5975.0 
               
               
                   
                 3rd 
                 115.5 
                 74.0 
                 200.0 
                 6421.7 
               
               
                   
                 Mean 
                 109.2 
                 81.7 
                 200.0 
                 6044.5 
               
               
                   
                 STDER 
                 3.4 
                 3.8 
                 0.0 
                 200.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 104.0 
                 85.0 
                 200.0 
                 5736.8 
               
               
                 YY101 0.05 nM 
                 2nd 
                 117.0 
                 74.0 
                 200.0 
                 6511.1 
               
               
                   
                 3rd 
                 104.5 
                 78.0 
                 200.0 
                 5766.6 
               
               
                   
                 Mean 
                 108.5 
                 79.0 
                 200.0 
                 6004.8 
               
               
                   
                 STDER 
                 4.3 
                 3.2 
                 0.0 
                 253.3 
               
               
                 PBMC + LPS(5 ug/ml) +M 
                 1st 
                 108.0 
                 71.0 
                 200.0 
                 5975.0 
               
               
                 YY101 0.005 nM 
                 2nd 
                 108.5 
                 64.0 
                 200.0 
                 6004.7 
               
               
                   
                 3rd 
                 99.0 
                 58.0 
                 200.0 
                 5439.3 
               
               
                   
                 Mean 
                 105.2 
                 64.3 
                 200.0 
                 5806.3 
               
               
                   
                 STDER 
                 3.1 
                 3.8 
                 0.0 
                 183.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 105.0 
                 81.0 
                 200.0 
                 5796.4 
               
               
                 YDE011 500 nM 
                 2nd 
                 52.5 
                 74.0 
                 200.0 
                 2681.1 
               
               
                   
                 3rd 
                 80.0 
                 70.0 
                 200.0 
                 4310.2 
               
               
                   
                 Mean 
                 79.2 
                 75.0 
                 200.0 
                 4262.6 
               
               
                   
                 STDER 
                 15.2 
                 3.2 
                 0.0 
                 899.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 101.0 
                 81.0 
                 200.0 
                 5558.3 
               
               
                 YDE011 50 nM 
                 2nd 
                 102.0 
                 81.0 
                 200.0 
                 5617.8 
               
               
                   
                 3rd 
                 61.0 
                 63.0 
                 200.0 
                 3183.9 
               
               
                   
                 Mean 
                 88.0 
                 75.0 
                 200.0 
                 4786.7 
               
               
                   
                 STDER 
                 13.5 
                 6.0 
                 0.0 
                 801.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 67.0 
                 74.0 
                 200.0 
                 3539.2 
               
               
                 YDE011 5 nM 
                 2nd 
                 79.0 
                 61.0 
                 200.0 
                 4250.8 
               
               
                   
                 3rd 
                 96.0 
                 70.0 
                 200.0 
                 5260.9 
               
               
                   
                 Mean 
                 80.7 
                 68.3 
                 200.0 
                 4350.3 
               
               
                   
                 STDER 
                 8.4 
                 3.8 
                 0.0 
                 499.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 89.0 
                 89.0 
                 200.0 
                 4844.7 
               
               
                 YDE011 0.5 nM 
                 2nd 
                 91.0 
                 79.0 
                 200.0 
                 4963.6 
               
               
                   
                 3rd 
                 88.0 
                 79.0 
                 200.0 
                 4785.3 
               
               
                   
                 Mean 
                 89.3 
                 82.3 
                 200.0 
                 4864.5 
               
               
                   
                 STDER 
                 0.9 
                 3.3 
                 0.0 
                 52.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 105.0 
                 82.0 
                 200.0 
                 5796.4 
               
               
                 YDE011 0.05 nM 
                 2nd 
                 110.0 
                 62.0 
                 200.0 
                 6094.1 
               
               
                   
                 3rd 
                 102.0 
                 83.0 
                 200.0 
                 5617.8 
               
               
                   
                 Mean 
                 105.7 
                 75.7 
                 200.0 
                 5836.1 
               
               
                   
                 STDER 
                 2.3 
                 6.8 
                 0.0 
                 138.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 97.5 
                 60.0 
                 200.0 
                 5350.1 
               
               
                 YDE011 0.005 nM 
                 2nd 
                 92.0 
                 86.0 
                 200.0 
                 5023.0 
               
               
                   
                 3rd 
                 94.0 
                 85.0 
                 200.0 
                 5141.9 
               
               
                   
                 Mean 
                 94.5 
                 77.0 
                 200.0 
                 5171.7 
               
               
                   
                 STDER 
                 1.6 
                 8.5 
                 0.0 
                 95.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 82.0 
                 70.0 
                 200.0 
                 4428.9 
               
               
                 YDE043 500 nM 
                 2nd 
                 78.0 
                 86.0 
                 200.0 
                 4191.5 
               
               
                   
                 3rd 
                 90.0 
                 76.0 
                 200.0 
                 4904.1 
               
               
                   
                 Mean 
                 83.3 
                 77.3 
                 200.0 
                 4508.2 
               
               
                   
                 STDER 
                 3.5 
                 4.7 
                 0.0 
                 209.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 101.5 
                 78.0 
                 200.0 
                 5588.1 
               
               
                 YDE043 50 nM 
                 2nd 
                 99.5 
                 78.0 
                 200.0 
                 5469.1 
               
               
                   
                 3rd 
                 88.0 
                 88.0 
                 200.0 
                 4785.3 
               
               
                   
                 Mean 
                 96.3 
                 81.3 
                 200.0 
                 5280.8 
               
               
                   
                 STDER 
                 4.2 
                 3.3 
                 0.0 
                 250.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 98.0 
                 59.0 
                 200.0 
                 5379.8 
               
               
                 YDE043 5 nM 
                 2nd 
                 101.0 
                 74.0 
                 200.0 
                 5558.3 
               
               
                   
                 3rd 
                 95.0 
                 86.0 
                 200.0 
                 5201.4 
               
               
                   
                 Mean 
                 98.0 
                 73.0 
                 200.0 
                 5379.8 
               
               
                   
                 STDER 
                 1.7 
                 7.8 
                 0.0 
                 103.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 91.0 
                 71.0 
                 200.0 
                 4963.6 
               
               
                 YDE043 0.5 nM 
                 2nd 
                 88.0 
                 83.0 
                 200.0 
                 4785.3 
               
               
                   
                 3rd 
                 82.0 
                 65.0 
                 200.0 
                 4428.9 
               
               
                   
                 Mean 
                 87.0 
                 73.0 
                 200.0 
                 4725.9 
               
               
                   
                 STDER 
                 2.6 
                 5.3 
                 0.0 
                 157.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 83.0 
                 73.0 
                 200.0 
                 4488.3 
               
               
                 YDE043 0.05 nM 
                 2nd 
                 82.0 
                 71.0 
                 200.0 
                 4428.9 
               
               
                   
                 3rd 
                 82.5 
                 80.0 
                 200.0 
                 4458.6 
               
               
                   
                 Mean 
                 82.5 
                 74.7 
                 200.0 
                 4458.6 
               
               
                   
                 STDER 
                 0.3 
                 2.7 
                 0.0 
                 17.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 84.0 
                 76.0 
                 200.0 
                 4547.7 
               
               
                 YDE043 0.005 nM 
                 2nd 
                 97.0 
                 65.0 
                 200.0 
                 5320.3 
               
               
                   
                 3rd 
                 119.0 
                 61.0 
                 200.0 
                 6630.3 
               
               
                   
                 Mean 
                 100.0 
                 67.3 
                 200.0 
                 5499.4 
               
               
                   
                 STDER 
                 10.2 
                 4.5 
                 0.0 
                 607.8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 20 
               
             
            
               
                   
               
               
                 Bead name: TIMP-1 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concen- 
               
               
                   
                   
                   
                   
                 Dilu- 
                 tration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 tion 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 13.0 
                 1.0 
                 50.0 
                 11.8 
               
               
                   
                 2nd 
                 13.0 
                 1.0 
                 52.0 
                 11.8 
               
               
                   
                 Mean 
                 13.0 
                 1.0 
                 51.0 
                 11.8 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 1.0 
                 0.0 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 26.5 
                 200.0 
                 50.0 
                 6531.0 
               
               
                   
                 2nd 
                 23.5 
                 200.0 
                 50.0 
                 5800.6 
               
               
                   
                 3rd 
                 22.0 
                 200.0 
                 52.0 
                 5411.1 
               
               
                   
                 Mean 
                 24.0 
                 200.0 
                 50.7 
                 5914.2 
               
               
                   
                 STDER 
                 1.3 
                 0.0 
                 0.7 
                 328.2 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 26.5 
                 200.0 
                 50.0 
                 6531.0 
               
               
                   
                 2nd 
                 25.5 
                 200.0 
                 50.0 
                 6293.9 
               
               
                   
                 3rd 
                 25.5 
                 200.0 
                 50.0 
                 6293.9 
               
               
                   
                 Mean 
                 25.8 
                 200.0 
                 50.0 
                 6372.9 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.0 
                 79.0 
               
               
                 PBMC + YY101 500 nM 
                 1st 
                 29.0 
                 200.0 
                 50.0 
                 7100.2 
               
               
                   
                 2nd 
                 30.0 
                 200.0 
                 50.0 
                 7319.6 
               
               
                   
                 Mean 
                 29.5 
                 200.0 
                 50.0 
                 7209.9 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 0.0 
                 109.7 
               
               
                 PBMC + YY101 50 nM 
                 1st 
                 29.5 
                 200.0 
                 50.0 
                 7210.4 
               
               
                   
                 2nd 
                 27.0 
                 200.0 
                 68.0 
                 6647.4 
               
               
                   
                 Mean 
                 28.3 
                 200.0 
                 59.0 
                 6928.9 
               
               
                   
                 STDER 
                 1.3 
                 0.0 
                 9.0 
                 281.5 
               
               
                 PBMC + YY101 5 nM 
                 1st 
                 26.5 
                 200.0 
                 50.0 
                 6531.0 
               
               
                   
                 2nd 
                 24.0 
                 200.0 
                 52.0 
                 5926.6 
               
               
                   
                 Mean 
                 25.3 
                 200.0 
                 51.0 
                 6228.8 
               
               
                   
                 STDER 
                 1.3 
                 0.0 
                 1.0 
                 302.2 
               
               
                 PBMC + YY101 0.5 nM 
                 1st 
                 25.0 
                 200.0 
                 50.0 
                 6173.1 
               
               
                   
                 2nd 
                 28.5 
                 200.0 
                 64.0 
                 6988.8 
               
               
                   
                 Mean 
                 26.8 
                 200.0 
                 57.0 
                 6580.9 
               
               
                   
                 STDER 
                 1.8 
                 0.0 
                 7.0 
                 407.8 
               
               
                 PBMC + YY101 0.05 uM 
                 1st 
                 46.0 
                 200.0 
                 54.0 
                 10402.5 
               
               
                   
                 2nd 
                 29.0 
                 200.0 
                 50.0 
                 7100.2 
               
               
                   
                 Mean 
                 37.5 
                 200.0 
                 52.0 
                 8751.3 
               
               
                   
                 STDER 
                 8.5 
                 0.0 
                 2.0 
                 1651.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 25.0 
                 200.0 
                 50.0 
                 6173.1 
               
               
                 Xiidra 500 nM 
                 2nd 
                 23.0 
                 200.0 
                 57.0 
                 5672.8 
               
               
                   
                 Mean 
                 24.0 
                 200.0 
                 53.5 
                 5923.0 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 3.5 
                 250.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 23.0 
                 200.0 
                 50.0 
                 5672.8 
               
               
                 Xiidra 50 nM 
                 2nd 
                 22.5 
                 200.0 
                 50.0 
                 5543.0 
               
               
                   
                 Mean 
                 22.8 
                 200.0 
                 50.0 
                 5607.9 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.0 
                 64.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 25.0 
                 200.0 
                 58.0 
                 6173.1 
               
               
                 Xiidra 5 nM 
                 2nd 
                 26.0 
                 200.0 
                 52.0 
                 6413.2 
               
               
                   
                 Mean 
                 25.5 
                 200.0 
                 55.0 
                 6293.1 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 3.0 
                 120.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 24.5 
                 200.0 
                 50.0 
                 6050.7 
               
               
                 Xiidra 0.5 nM 
                 2nd 
                 25.0 
                 200.0 
                 50.0 
                 6173.1 
               
               
                   
                 Mean 
                 24.8 
                 200.0 
                 50.0 
                 6111.9 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.0 
                 61.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 23.5 
                 200.0 
                 50.0 
                 5800.6 
               
               
                 Xiidra 0.05 nM 
                 2nd 
                 24.0 
                 200.0 
                 55.0 
                 5926.6 
               
               
                   
                 Mean 
                 23.8 
                 200.0 
                 52.5 
                 5863.6 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 2.5 
                 63.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 24.0 
                 200.0 
                 50.0 
                 5926.6 
               
               
                 Xiidra 0.005 nM 
                 2nd 
                 22.5 
                 200.0 
                 50.0 
                 5543.0 
               
               
                   
                 Mean 
                 23.3 
                 200.0 
                 50.0 
                 5734.8 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 0.0 
                 191.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 17.0 
                 200.0 
                 52.0 
                 3935.4 
               
               
                 YY101 500 nM 
                 2nd 
                 17.0 
                 200.0 
                 67.0 
                 3935.4 
               
               
                   
                 3rd 
                 15.0 
                 200.0 
                 50.0 
                 3220.1 
               
               
                   
                 Mean 
                 16.3 
                 200.0 
                 56.3 
                 3696.9 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 5.4 
                 238.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 17.0 
                 200.0 
                 50.0 
                 3935.4 
               
               
                 YY101 50 nM 
                 2nd 
                 15.0 
                 200.0 
                 50.0 
                 3220.1 
               
               
                   
                 3rd 
                 17.0 
                 200.0 
                 54.0 
                 3935.4 
               
               
                   
                 Mean 
                 16.3 
                 200.0 
                 51.3 
                 3696.9 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 1.3 
                 238.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 17.0 
                 200.0 
                 50.0 
                 3935.4 
               
               
                 YY101 5 nM 
                 2nd 
                 17.0 
                 200.0 
                 57.0 
                 3935.4 
               
               
                   
                 3rd 
                 15.0 
                 200.0 
                 50.0 
                 3220.1 
               
               
                   
                 Mean 
                 16.3 
                 200.0 
                 52.3 
                 3696.9 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 2.3 
                 238.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 17.0 
                 200.0 
                 50.0 
                 3935.4 
               
               
                 YY101 0.5 nM 
                 2nd 
                 16.0 
                 200.0 
                 50.0 
                 3590.9 
               
               
                   
                 3rd 
                 20.0 
                 200.0 
                 50.0 
                 4859.3 
               
               
                   
                 Mean 
                 17.7 
                 200.0 
                 50.0 
                 4128.5 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 0.0 
                 378.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 17.0 
                 200.0 
                 55.0 
                 3935.4 
               
               
                 YY101 0.05 nM 
                 2nd 
                 16.0 
                 200.0 
                 50.0 
                 3590.9 
               
               
                   
                 3rd 
                 17.0 
                 200.0 
                 56.0 
                 3935.4 
               
               
                   
                 Mean 
                 16.7 
                 200.0 
                 53.7 
                 3820.5 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 1.9 
                 114.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 18.0 
                 200.0 
                 50.0 
                 4259.2 
               
               
                 YY101 0.005 nM 
                 2nd 
                 15.0 
                 200.0 
                 54.0 
                 3220.1 
               
               
                   
                 3rd 
                 18.0 
                 200.0 
                 57.0 
                 4259.2 
               
               
                   
                 Mean 
                 17.0 
                 200.0 
                 53.7 
                 3912.8 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 2.0 
                 346.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 17.0 
                 200.0 
                 50.0 
                 3935.4 
               
               
                 YDE011 500 nM 
                 2nd 
                 16.0 
                 200.0 
                 50.0 
                 3590.9 
               
               
                   
                 3rd 
                 17.0 
                 200.0 
                 52.0 
                 3935.4 
               
               
                   
                 Mean 
                 16.7 
                 200.0 
                 50.7 
                 3820.5 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.7 
                 114.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 17.0 
                 200.0 
                 50.0 
                 3935.4 
               
               
                 YDE011 50 nM 
                 2nd 
                 18.0 
                 200.0 
                 50.0 
                 4259.2 
               
               
                   
                 3rd 
                 19.0 
                 200.0 
                 55.0 
                 4566.3 
               
               
                   
                 Mean 
                 18.0 
                 200.0 
                 51.7 
                 4253.6 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 1.7 
                 182.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 20.0 
                 200.0 
                 50.0 
                 4859.3 
               
               
                 YDE011 5 nM 
                 2nd 
                 17.0 
                 200.0 
                 50.0 
                 3935.4 
               
               
                   
                 3rd 
                 16.0 
                 200.0 
                 50.0 
                 3590.9 
               
               
                   
                 Mean 
                 17.7 
                 200.0 
                 50.0 
                 4128.5 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 0.0 
                 378.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 16.0 
                 200.0 
                 50.0 
                 3590.9 
               
               
                 YDE011 0.5 nM 
                 2nd 
                 17.0 
                 200.0 
                 50.0 
                 3935.4 
               
               
                   
                 3rd 
                 19.5 
                 200.0 
                 54.0 
                 4714.4 
               
               
                   
                 Mean 
                 17.5 
                 200.0 
                 51.3 
                 4080.2 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 1.3 
                 332.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 18.5 
                 200.0 
                 50.0 
                 4414.6 
               
               
                 YDE011 0.05 nM 
                 2nd 
                 16.0 
                 200.0 
                 50.0 
                 3590.9 
               
               
                   
                 3rd 
                 20.0 
                 200.0 
                 50.0 
                 4859.3 
               
               
                   
                 Mean 
                 18.2 
                 200.0 
                 50.0 
                 4288.3 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 0.0 
                 371.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 18.0 
                 200.0 
                 50.0 
                 4259.2 
               
               
                 YDE011 0.005 nM 
                 2nd 
                 16.0 
                 200.0 
                 50.0 
                 3590.9 
               
               
                   
                 3rd 
                 18.0 
                 200.0 
                 50.0 
                 4259.2 
               
               
                   
                 Mean 
                 17.3 
                 200.0 
                 50.0 
                 4036.4 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 0.0 
                 222.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 19.0 
                 200.0 
                 60.0 
                 4566.3 
               
               
                 YDE043 500 nM 
                 2nd 
                 16.5 
                 200.0 
                 50.0 
                 3766.0 
               
               
                   
                 3rd 
                 19.0 
                 200.0 
                 50.0 
                 4566.3 
               
               
                   
                 Mean 
                 18.2 
                 200.0 
                 53.3 
                 4299.5 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 3.3 
                 266.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 17.0 
                 200.0 
                 50.0 
                 3935.4 
               
               
                 YDE043 50 nM 
                 2nd 
                 20.0 
                 200.0 
                 50.0 
                 4859.3 
               
               
                   
                 3rd 
                 18.0 
                 200.0 
                 58.0 
                 4259.2 
               
               
                   
                 Mean 
                 18.3 
                 200.0 
                 52.7 
                 4351.3 
               
               
                   
                 STDER 
                 0.9 
                 0.0 
                 2.7 
                 270.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 19.0 
                 200.0 
                 58.0 
                 4566.3 
               
               
                 YDE043 5 nM 
                 2nd 
                 19.0 
                 200.0 
                 60.0 
                 4566.3 
               
               
                   
                 3rd 
                 18.0 
                 200.0 
                 54.0 
                 4259.2 
               
               
                   
                 Mean 
                 18.7 
                 200.0 
                 57.3 
                 4463.9 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 1.8 
                 102.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 18.0 
                 200.0 
                 56.0 
                 4259.2 
               
               
                 YDE043 0.5 nM 
                 2nd 
                 18.5 
                 200.0 
                 50.0 
                 4414.6 
               
               
                   
                 3rd 
                 17.0 
                 200.0 
                 51.0 
                 3935.4 
               
               
                   
                 Mean 
                 17.8 
                 200.0 
                 52.3 
                 4203.1 
               
               
                   
                 STDER 
                 0.4 
                 0.0 
                 1.9 
                 141.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 18.0 
                 200.0 
                 50.0 
                 4259.2 
               
               
                 YDE043 0.05 nM 
                 2nd 
                 18.5 
                 200.0 
                 50.0 
                 4414.6 
               
               
                   
                 3rd 
                 16.0 
                 200.0 
                 50.0 
                 3590.9 
               
               
                   
                 Mean 
                 17.5 
                 200.0 
                 50.0 
                 4088.2 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 0.0 
                 252.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 17.0 
                 200.0 
                 50.0 
                 3935.4 
               
               
                 YDE043 0.005 nM 
                 2nd 
                 22.5 
                 200.0 
                 50.0 
                 5543.0 
               
               
                   
                 3rd 
                 19.0 
                 200.0 
                 54.0 
                 4566.3 
               
               
                   
                 Mean 
                 19.5 
                 200.0 
                 51.3 
                 4681.6 
               
               
                   
                 STDER 
                 1.6 
                 0.0 
                 1.3 
                 467.7 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 21 
               
             
            
               
                   
               
               
                 Bead name: Leptin 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concen- 
               
               
                   
                   
                   
                   
                 Dilu- 
                 tration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 tion 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 24.0 
                 71.0 
                 1.0 
                 110.4 
               
               
                   
                 2nd 
                 23.0 
                 63.0 
                 1.0 
                 99.0 
               
               
                   
                 Mean 
                 23.5 
                 67.0 
                 1.0 
                 104.7 
               
               
                   
                 STDER 
                 0.5 
                 4.0 
                 0.0 
                 5.7 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 23.0 
                 63.0 
                 200.0 
                 19789.4 
               
               
                   
                 2nd 
                 24.0 
                 77.0 
                 200.0 
                 22079.2 
               
               
                   
                 3rd 
                 22.0 
                 73.0 
                 200.0 
                 17222.7 
               
               
                   
                 Mean 
                 23.0 
                 71.0 
                 200.0 
                 19697.1 
               
               
                   
                 STDER 
                 0.6 
                 4.2 
                 0.0 
                 1402.7 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 23.0 
                 56.0 
                 200.0 
                 19789.4 
               
               
                   
                 2nd 
                 22.0 
                 51.0 
                 200.0 
                 17222.7 
               
               
                   
                 3rd 
                 24.0 
                 64.0 
                 200.0 
                 22079.2 
               
               
                   
                 Mean 
                 23.0 
                 57.0 
                 200.0 
                 19697.1 
               
               
                   
                 STDER 
                 0.6 
                 3.8 
                 0.0 
                 1402.7 
               
               
                 PBMC + YY101 500 nM 
                 1st 
                 23.0 
                 88.0 
                 200.0 
                 19789.4 
               
               
                   
                 2nd 
                 23.5 
                 84.0 
                 200.0 
                 20963.1 
               
               
                   
                 Mean 
                 23.3 
                 86.0 
                 200.0 
                 20376.3 
               
               
                   
                 STDER 
                 0.3 
                 2.0 
                 0.0 
                 586.9 
               
               
                 PBMC + YY101 50 nM 
                 1st 
                 26.0 
                 73.0 
                 200.0 
                 26106.3 
               
               
                   
                 2nd 
                 24.0 
                 63.0 
                 200.0 
                 22079.2 
               
               
                   
                 Mean 
                 25.0 
                 68.0 
                 200.0 
                 24092.8 
               
               
                   
                 STDER 
                 1.0 
                 5.0 
                 0.0 
                 2013.5 
               
               
                 PBMC + YY101 5 nM 
                 1st 
                 22.0 
                 74.0 
                 200.0 
                 17222.7 
               
               
                   
                 2nd 
                 23.5 
                 58.0 
                 200.0 
                 20963.1 
               
               
                   
                 Mean 
                 22.8 
                 66.0 
                 200.0 
                 19092.9 
               
               
                   
                 STDER 
                 0.8 
                 8.0 
                 0.0 
                 1870.2 
               
               
                 PBMC + YY101 0.5 nM 
                 1st 
                 21.0 
                 55.0 
                 200.0 
                 14234.9 
               
               
                   
                 2nd 
                 22.0 
                 62.0 
                 200.0 
                 17222.7 
               
               
                   
                 Mean 
                 21.5 
                 58.5 
                 200.0 
                 15728.8 
               
               
                   
                 STDER 
                 0.5 
                 3.5 
                 0.0 
                 1493.9 
               
               
                 PBMC + YY101 0.05 uM 
                 1st 
                 23.0 
                 72.0 
                 200.0 
                 19789.4 
               
               
                   
                 2nd 
                 22.0 
                 61.0 
                 200.0 
                 17222.7 
               
               
                   
                 Mean 
                 22.5 
                 66.5 
                 200.0 
                 18506.0 
               
               
                   
                 STDER 
                 0.5 
                 5.5 
                 0.0 
                 1283.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 26.0 
                 50.0 
                 200.0 
                 26106.3 
               
               
                 Xiidra 500 nM 
                 2nd 
                 22.0 
                 60.0 
                 200.0 
                 17222.7 
               
               
                   
                 Mean 
                 24.0 
                 55.0 
                 200.0 
                 21664.5 
               
               
                   
                 STDER 
                 2.0 
                 5.0 
                 0.0 
                 4441.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 25.0 
                 53.0 
                 200.0 
                 24169.3 
               
               
                 Xiidra 50 nM 
                 2nd 
                 22.0 
                 92.0 
                 200.0 
                 17222.7 
               
               
                   
                 Mean 
                 23.5 
                 72.5 
                 200.0 
                 20696.0 
               
               
                   
                 STDER 
                 1.5 
                 19.5 
                 0.0 
                 3473.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 22.0 
                 77.0 
                 200.0 
                 17222.7 
               
               
                 Xiidra 5 nM 
                 2nd 
                 22.0 
                 50.0 
                 200.0 
                 17222.7 
               
               
                   
                 Mean 
                 22.0 
                 63.5 
                 200.0 
                 17222.7 
               
               
                   
                 STDER 
                 0.0 
                 13.5 
                 0.0 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 20.0 
                 72.0 
                 200.0 
                 10481.4 
               
               
                 Xiidra 0.5 nM 
                 2nd 
                 20.0 
                 66.0 
                 200.0 
                 10481.4 
               
               
                   
                 Mean 
                 20.0 
                 69.0 
                 200.0 
                 10481.4 
               
               
                   
                 STDER 
                 0.0 
                 3.0 
                 0.0 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 22.0 
                 81.0 
                 200.0 
                 17222.7 
               
               
                 Xiidra 0.05 nM 
                 2nd 
                 22.5 
                 58.0 
                 200.0 
                 18547.4 
               
               
                   
                 Mean 
                 22.3 
                 69.5 
                 200.0 
                 17885.0 
               
               
                   
                 STDER 
                 0.3 
                 11.5 
                 0.0 
                 662.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 21.0 
                 66.0 
                 200.0 
                 14234.9 
               
               
                 Xiidra 0.005 nM 
                 2nd 
                 22.0 
                 55.0 
                 200.0 
                 17222.7 
               
               
                   
                 Mean 
                 21.5 
                 60.5 
                 200.0 
                 15728.8 
               
               
                   
                 STDER 
                 0.5 
                 5.5 
                 0.0 
                 1493.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 23.0 
                 53.0 
                 200.0 
                 19789.4 
               
               
                 YY101 500 nM 
                 2nd 
                 22.0 
                 72.0 
                 200.0 
                 17222.7 
               
               
                   
                 3rd 
                 23.0 
                 66.0 
                 200.0 
                 19789.4 
               
               
                   
                 Mean 
                 22.7 
                 63.7 
                 200.0 
                 18933.8 
               
               
                   
                 STDER 
                 0.3 
                 5.6 
                 0.0 
                 855.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 22.0 
                 59.0 
                 200.0 
                 17222.7 
               
               
                 YY101 50 nM 
                 2nd 
                 21.0 
                 65.0 
                 200.0 
                 14234.9 
               
               
                   
                 3rd 
                 21.0 
                 71.0 
                 200.0 
                 14234.9 
               
               
                   
                 Mean 
                 21.3 
                 65.0 
                 200.0 
                 15230.8 
               
               
                   
                 STDER 
                 0.3 
                 3.5 
                 0.0 
                 995.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 24.0 
                 60.0 
                 200.0 
                 22079.2 
               
               
                 YY101 5 nM 
                 2nd 
                 22.0 
                 74.0 
                 200.0 
                 17222.7 
               
               
                   
                 3rd 
                 25.0 
                 63.0 
                 200.0 
                 24169.3 
               
               
                   
                 Mean 
                 23.7 
                 65.7 
                 200.0 
                 21157.1 
               
               
                   
                 STDER 
                 0.9 
                 4.3 
                 0.0 
                 2057.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 22.0 
                 52.0 
                 200.0 
                 17222.7 
               
               
                 YY101 0.5 nM 
                 2nd 
                 22.0 
                 71.0 
                 200.0 
                 17222.7 
               
               
                   
                 3rd 
                 21.0 
                 81.0 
                 200.0 
                 14234.9 
               
               
                   
                 Mean 
                 21.7 
                 68.0 
                 200.0 
                 16226.8 
               
               
                   
                 STDER 
                 0.3 
                 8.5 
                 0.0 
                 995.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 22.0 
                 56.0 
                 200.0 
                 17222.7 
               
               
                 YY101 0.05 nM 
                 2nd 
                 21.5 
                 70.0 
                 200.0 
                 15795.3 
               
               
                   
                 3rd 
                 22.0 
                 52.0 
                 200.0 
                 17222.7 
               
               
                   
                 Mean 
                 21.8 
                 59.3 
                 200.0 
                 16746.9 
               
               
                   
                 STDER 
                 0.2 
                 5.5 
                 0.0 
                 475.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 23.0 
                 62.0 
                 200.0 
                 19789.4 
               
               
                 YY101 0.005 nM 
                 2nd 
                 20.0 
                 54.0 
                 200.0 
                 10481.4 
               
               
                   
                 3rd 
                 23.0 
                 59.0 
                 200.0 
                 19789.4 
               
               
                   
                 Mean 
                 22.0 
                 58.3 
                 200.0 
                 16686.7 
               
               
                   
                 STDER 
                 1.0 
                 2.3 
                 0.0 
                 3102.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 23.0 
                 63.0 
                 200.0 
                 19789.4 
               
               
                 YDE011 500 nM 
                 2nd 
                 13.0 
                 79.0 
                 200.0 
                 &lt;98.95 
               
               
                   
                 3rd 
                 19.5 
                 62.0 
                 200.0 
                 8003.2 
               
               
                   
                 Mean 
                 18.5 
                 68.0 
                 200.0 
                 13896.3 
               
               
                   
                 STDER 
                 2.9 
                 5.5 
                 0.0 
                 5893.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 20.0 
                 66.0 
                 200.0 
                 10481.4 
               
               
                 YDE011 50 nM 
                 2nd 
                 21.0 
                 65.0 
                 200.0 
                 14234.9 
               
               
                   
                 3rd 
                 12.0 
                 60.0 
                 200.0 
                 &lt;98.95 
               
               
                   
                 Mean 
                 17.7 
                 63.7 
                 200.0 
                 12358.1 
               
               
                   
                 STDER 
                 2.8 
                 1.9 
                 0.0 
                 1876.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 17.0 
                 52.0 
                 200.0 
                 &lt;98.95 
               
               
                 YDE011 5 nM 
                 2nd 
                 19.0 
                 60.0 
                 200.0 
                 4332.6 
               
               
                   
                 3rd 
                 22.0 
                 62.0 
                 200.0 
                 17222.7 
               
               
                   
                 Mean 
                 19.3 
                 58.0 
                 200.0 
                 10777.7 
               
               
                   
                 STDER 
                 1.5 
                 3.1 
                 0.0 
                 6445.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 20.0 
                 76.0 
                 200.0 
                 10481.4 
               
               
                 YDE011 0.5 nM 
                 2nd 
                 19.0 
                 91.0 
                 200.0 
                 4332.6 
               
               
                   
                 3rd 
                 21.0 
                 67.0 
                 200.0 
                 14234.9 
               
               
                   
                 Mean 
                 20.0 
                 78.0 
                 200.0 
                 9683.0 
               
               
                   
                 STDER 
                 0.6 
                 7.0 
                 0.0 
                 2886.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 23.0 
                 74.0 
                 200.0 
                 19789.4 
               
               
                 YDE011 0.05 nM 
                 2nd 
                 20.0 
                 80.0 
                 200.0 
                 10481.4 
               
               
                   
                 3rd 
                 21.0 
                 81.0 
                 200.0 
                 14234.9 
               
               
                   
                 Mean 
                 21.3 
                 78.3 
                 200.0 
                 14835.2 
               
               
                   
                 STDER 
                 0.9 
                 2.2 
                 0.0 
                 2703.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 22.0 
                 50.0 
                 200.0 
                 17222.7 
               
               
                 YDE011 0.005 nM 
                 2nd 
                 22.0 
                 59.0 
                 200.0 
                 17222.7 
               
               
                   
                 3rd 
                 20.0 
                 75.0 
                 200.0 
                 10481.4 
               
               
                   
                 Mean 
                 21.3 
                 61.3 
                 200.0 
                 14975.6 
               
               
                   
                 STDER 
                 0.7 
                 7.3 
                 0.0 
                 2247.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 22.0 
                 63.0 
                 200.0 
                 17222.7 
               
               
                 YDE043 500 nM 
                 2nd 
                 19.0 
                 65.0 
                 200.0 
                 4332.6 
               
               
                   
                 3rd 
                 20.0 
                 50.0 
                 200.0 
                 10481.4 
               
               
                   
                 Mean 
                 20.3 
                 59.3 
                 200.0 
                 10678.9 
               
               
                   
                 STDER 
                 0.9 
                 4.7 
                 0.0 
                 3722.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 21.5 
                 54.0 
                 200.0 
                 15795.3 
               
               
                 YDE043 50 nM 
                 2nd 
                 23.0 
                 65.0 
                 200.0 
                 19789.4 
               
               
                   
                 3rd 
                 23.0 
                 70.0 
                 200.0 
                 19789.4 
               
               
                   
                 Mean 
                 22.5 
                 63.0 
                 200.0 
                 18458.0 
               
               
                   
                 STDER 
                 0.5 
                 4.7 
                 0.0 
                 1331.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 23.0 
                 55.0 
                 200.0 
                 19789.4 
               
               
                 YDE043 5 nM 
                 2nd 
                 20.5 
                 60.0 
                 200.0 
                 12493.0 
               
               
                   
                 3rd 
                 21.0 
                 79.0 
                 200.0 
                 14234.9 
               
               
                   
                 Mean 
                 21.5 
                 64.7 
                 200.0 
                 15505.7 
               
               
                   
                 STDER 
                 0.8 
                 7.3 
                 0.0 
                 2200.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 20.5 
                 54.0 
                 200.0 
                 12493.0 
               
               
                 YDE043 0.5 nM 
                 2nd 
                 22.0 
                 64.0 
                 200.0 
                 17222.7 
               
               
                   
                 3rd 
                 22.0 
                 63.0 
                 200.0 
                 17222.7 
               
               
                   
                 Mean 
                 21.5 
                 60.3 
                 200.0 
                 15646.1 
               
               
                   
                 STDER 
                 0.5 
                 3.2 
                 0.0 
                 1576.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 20.0 
                 63.0 
                 200.0 
                 10481.4 
               
               
                 YDE043 0.05 nM 
                 2nd 
                 18.5 
                 60.0 
                 200.0 
                 &lt;98.95 
               
               
                   
                 3rd 
                 18.0 
                 62.0 
                 200.0 
                 &lt;98.95 
               
               
                   
                 Mean 
                 18.8 
                 61.7 
                 200.0 
                 10481.4 
               
               
                   
                 STDER 
                 0.6 
                 0.9 
                 0.0 
                 #DIV/0! 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 22.5 
                 58.0 
                 200.0 
                 18547.4 
               
               
                 YDE043 0.005 nM 
                 2nd 
                 24.0 
                 60.0 
                 200.0 
                 22079.2 
               
               
                   
                 3rd 
                 22.5 
                 56.0 
                 200.0 
                 18547.4 
               
               
                   
                 Mean 
                 23.0 
                 58.0 
                 200.0 
                 19724.6 
               
               
                   
                 STDER 
                 0.5 
                 1.2 
                 0.0 
                 1177.3 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 22 
               
             
            
               
                   
               
               
                 Bead name: MMP-9 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Concen- 
               
               
                   
                   
                   
                   
                 Dilu- 
                 tration 
               
               
                 Sample 
                   
                 MFI 
                 Count 
                 tion 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 29.5 
                 50.0 
                 1.0 
                 20.7 
               
               
                   
                 2nd 
                 34.0 
                 64.0 
                 1.0 
                 27.8 
               
               
                   
                 Mean 
                 31.8 
                 57.0 
                 1.0 
                 24.3 
               
               
                   
                 STDER 
                 2.3 
                 7.0 
                 0.0 
                 3.5 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 56.0 
                 66.0 
                 200.0 
                 12836.1 
               
               
                   
                 2nd 
                 39.0 
                 76.0 
                 200.0 
                 7158.5 
               
               
                   
                 3rd 
                 37.0 
                 93.0 
                 200.0 
                 6512.8 
               
               
                   
                 Mean 
                 44.0 
                 78.3 
                 200.0 
                 8835.8 
               
               
                   
                 STDER 
                 6.0 
                 7.9 
                 0.0 
                 2008.8 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 33.0 
                 87.0 
                 200.0 
                 5239.4 
               
               
                   
                 2nd 
                 35.0 
                 77.0 
                 200.0 
                 5872.9 
               
               
                   
                 3rd 
                 33.0 
                 83.0 
                 200.0 
                 5239.4 
               
               
                   
                 Mean 
                 33.7 
                 82.3 
                 200.0 
                 5450.5 
               
               
                   
                 STDER 
                 0.7 
                 2.9 
                 0.0 
                 211.2 
               
               
                 PBMC + YY101 500 nM 
                 1st 
                 48.0 
                 69.0 
                 200.0 
                 10126.8 
               
               
                   
                 2nd 
                 54.0 
                 92.0 
                 200.0 
                 12153.3 
               
               
                   
                 Mean 
                 51.0 
                 80.5 
                 200.0 
                 11140.0 
               
               
                   
                 STDER 
                 3.0 
                 11.5 
                 0.0 
                 1013.3 
               
               
                 PBMC + YY101 50 nM 
                 1st 
                 53.0 
                 89.0 
                 200.0 
                 11813.2 
               
               
                   
                 2nd 
                 46.0 
                 70.0 
                 200.0 
                 9459.2 
               
               
                   
                 Mean 
                 49.5 
                 79.5 
                 200.0 
                 10636.2 
               
               
                   
                 STDER 
                 3.5 
                 9.5 
                 0.0 
                 1177.0 
               
               
                 PBMC + YY101 5 nM 
                 1st 
                 67.5 
                 78.0 
                 200.0 
                 16823.6 
               
               
                   
                 2nd 
                 52.0 
                 81.0 
                 200.0 
                 11474.0 
               
               
                   
                 Mean 
                 59.8 
                 79.5 
                 200.0 
                 14148.8 
               
               
                   
                 STDER 
                 7.8 
                 1.5 
                 0.0 
                 2674.8 
               
               
                 PBMC + YY101 0.5 nM 
                 1st 
                 56.0 
                 82.0 
                 200.0 
                 12836.1 
               
               
                   
                 2nd 
                 77.0 
                 73.0 
                 200.0 
                 20186.4 
               
               
                   
                 Mean 
                 66.5 
                 77.5 
                 200.0 
                 16511.2 
               
               
                   
                 STDER 
                 10.5 
                 4.5 
                 0.0 
                 3675.1 
               
               
                 PBMC + YY101 0.05 uM 
                 1st 
                 79.0 
                 69.0 
                 200.0 
                 20901.3 
               
               
                   
                 2nd 
                 57.0 
                 74.0 
                 200.0 
                 13178.7 
               
               
                   
                 Mean 
                 68.0 
                 71.5 
                 200.0 
                 17040.0 
               
               
                   
                 STDER 
                 11.0 
                 2.5 
                 0.0 
                 3861.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 35.0 
                 73.0 
                 200.0 
                 5872.9 
               
               
                 Xiidra 500 nM 
                 2nd 
                 35.0 
                 65.0 
                 200.0 
                 5872.9 
               
               
                   
                 Mean 
                 35.0 
                 69.0 
                 200.0 
                 5872.9 
               
               
                   
                 STDER 
                 0.0 
                 4.0 
                 0.0 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 35.0 
                 63.0 
                 200.0 
                 5872.9 
               
               
                 Xiidra 50 nM 
                 2nd 
                 37.0 
                 79.0 
                 200.0 
                 6512.8 
               
               
                   
                 Mean 
                 36.0 
                 71.0 
                 200.0 
                 6192.8 
               
               
                   
                 STDER 
                 1.0 
                 8.0 
                 0.0 
                 319.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 32.0 
                 76.0 
                 200.0 
                 4925.2 
               
               
                 Xiidra 5 nM 
                 2nd 
                 37.0 
                 85.0 
                 200.0 
                 6512.8 
               
               
                   
                 Mean 
                 34.5 
                 80.5 
                 200.0 
                 5719.0 
               
               
                   
                 STDER 
                 2.5 
                 4.5 
                 0.0 
                 793.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 27.5 
                 72.0 
                 200.0 
                 3535.8 
               
               
                 Xiidra 0.5 nM 
                 2nd 
                 31.0 
                 83.0 
                 200.0 
                 4612.9 
               
               
                   
                 Mean 
                 29.3 
                 77.5 
                 200.0 
                 4074.3 
               
               
                   
                 STDER 
                 1.8 
                 5.5 
                 0.0 
                 538.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 30.0 
                 102.0 
                 200.0 
                 4302.5 
               
               
                 Xiidra 0.05 nM 
                 2nd 
                 31.5 
                 78.0 
                 200.0 
                 4768.8 
               
               
                   
                 Mean 
                 30.8 
                 90.0 
                 200.0 
                 4535.6 
               
               
                   
                 STDER 
                 0.8 
                 12.0 
                 0.0 
                 233.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 30.0 
                 77.0 
                 200.0 
                 4302.5 
               
               
                 Xiidra 0.005 nM 
                 2nd 
                 30.0 
                 76.0 
                 200.0 
                 4302.5 
               
               
                   
                 Mean 
                 30.0 
                 76.5 
                 200.0 
                 4302.5 
               
               
                   
                 STDER 
                 0.0 
                 0.5 
                 0.0 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 31.0 
                 64.0 
                 200.0 
                 4612.9 
               
               
                 YY101 500 nM 
                 2nd 
                 33.0 
                 71.0 
                 200.0 
                 5239.4 
               
               
                   
                 3rd 
                 34.0 
                 64.0 
                 200.0 
                 5555.3 
               
               
                   
                 Mean 
                 32.7 
                 66.3 
                 200.0 
                 5135.8 
               
               
                   
                 STDER 
                 0.9 
                 2.3 
                 0.0 
                 276.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 33.0 
                 80.0 
                 200.0 
                 5239.4 
               
               
                 YY101 50 nM 
                 2nd 
                 32.0 
                 76.0 
                 200.0 
                 4925.2 
               
               
                   
                 3rd 
                 34.0 
                 76.0 
                 200.0 
                 5555.3 
               
               
                   
                 Mean 
                 33.0 
                 77.3 
                 200.0 
                 5239.9 
               
               
                   
                 STDER 
                 0.6 
                 1.3 
                 0.0 
                 181.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 33.0 
                 76.0 
                 200.0 
                 5239.4 
               
               
                 YY101 5 nM 
                 2nd 
                 30.0 
                 74.0 
                 200.0 
                 4302.5 
               
               
                   
                 3rd 
                 35.0 
                 79.0 
                 200.0 
                 5872.9 
               
               
                   
                 Mean 
                 32.7 
                 76.3 
                 200.0 
                 5138.2 
               
               
                   
                 STDER 
                 1.5 
                 1.5 
                 0.0 
                 456.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 33.0 
                 73.0 
                 200.0 
                 5239.4 
               
               
                 YY101 0.5 nM 
                 2nd 
                 33.0 
                 74.0 
                 200.0 
                 5239.4 
               
               
                   
                 3rd 
                 33.0 
                 60.0 
                 200.0 
                 5239.4 
               
               
                   
                 Mean 
                 33.0 
                 69.0 
                 200.0 
                 5239.4 
               
               
                   
                 STDER 
                 0.0 
                 4.5 
                 0.0 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 33.0 
                 69.0 
                 200.0 
                 5239.4 
               
               
                 YY101 0.05 nM 
                 2nd 
                 32.0 
                 77.0 
                 200.0 
                 4925.2 
               
               
                   
                 3rd 
                 31.5 
                 68.0 
                 200.0 
                 4768.8 
               
               
                   
                 Mean 
                 32.2 
                 71.3 
                 200.0 
                 4977.8 
               
               
                   
                 STDER 
                 0.4 
                 2.8 
                 0.0 
                 138.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 28.0 
                 76.0 
                 200.0 
                 3688.0 
               
               
                 YY101 0.005 nM 
                 2nd 
                 32.0 
                 69.0 
                 200.0 
                 4925.2 
               
               
                   
                 3rd 
                 33.0 
                 57.0 
                 200.0 
                 5239.4 
               
               
                   
                 Mean 
                 31.0 
                 67.3 
                 200.0 
                 4617.5 
               
               
                   
                 STDER 
                 1.5 
                 5.5 
                 0.0 
                 473.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 31.0 
                 75.0 
                 200.0 
                 4612.9 
               
               
                 YDE011 500 nM 
                 2nd 
                 18.5 
                 76.0 
                 200.0 
                 931.9 
               
               
                   
                 3rd 
                 25.0 
                 77.0 
                 200.0 
                 2784.3 
               
               
                   
                 Mean 
                 24.8 
                 76.0 
                 200.0 
                 2776.4 
               
               
                   
                 STDER 
                 3.6 
                 0.6 
                 0.0 
                 1062.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 24.5 
                 74.0 
                 200.0 
                 2636.1 
               
               
                 YDE011 50 nM 
                 2nd 
                 28.0 
                 71.0 
                 200.0 
                 3688.0 
               
               
                   
                 3rd 
                 20.0 
                 62.0 
                 200.0 
                 1342.1 
               
               
                   
                 Mean 
                 24.2 
                 69.0 
                 200.0 
                 2555.4 
               
               
                   
                 STDER 
                 2.3 
                 3.6 
                 0.0 
                 678.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 22.0 
                 77.0 
                 200.0 
                 1907.3 
               
               
                 YDE011 5 nM 
                 2nd 
                 28.0 
                 65.0 
                 200.0 
                 3688.0 
               
               
                   
                 3rd 
                 30.0 
                 77.0 
                 200.0 
                 4302.5 
               
               
                   
                 Mean 
                 26.7 
                 73.0 
                 200.0 
                 3299.3 
               
               
                   
                 STDER 
                 2.4 
                 4.0 
                 0.0 
                 718.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 27.0 
                 92.0 
                 200.0 
                 3384.2 
               
               
                 YDE011 0.5 nM 
                 2nd 
                 28.0 
                 80.0 
                 200.0 
                 3688.0 
               
               
                   
                 3rd 
                 26.5 
                 66.0 
                 200.0 
                 3233.2 
               
               
                   
                 Mean 
                 27.2 
                 79.3 
                 200.0 
                 3435.2 
               
               
                   
                 STDER 
                 0.4 
                 7.5 
                 0.0 
                 133.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 31.0 
                 65.0 
                 200.0 
                 4612.9 
               
               
                 YDE011 0.05 nM 
                 2nd 
                 31.0 
                 79.0 
                 200.0 
                 4612.9 
               
               
                   
                 3rd 
                 30.0 
                 80.0 
                 200.0 
                 4302.5 
               
               
                   
                 Mean 
                 30.7 
                 74.7 
                 200.0 
                 4509.4 
               
               
                   
                 STDER 
                 0.3 
                 4.8 
                 0.0 
                 103.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 29.5 
                 60.0 
                 200.0 
                 4148.1 
               
               
                 YDE011 0.005 nM 
                 2nd 
                 31.0 
                 60.0 
                 200.0 
                 4612.9 
               
               
                   
                 3rd 
                 29.0 
                 82.0 
                 200.0 
                 3994.2 
               
               
                   
                 Mean 
                 29.8 
                 67.3 
                 200.0 
                 4251.7 
               
               
                   
                 STDER 
                 0.6 
                 7.3 
                 0.0 
                 186.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 28.0 
                 72.0 
                 200.0 
                 3688.0 
               
               
                 YDE043 500 nM 
                 2nd 
                 32.0 
                 70.0 
                 200.0 
                 4925.2 
               
               
                   
                 3rd 
                 28.0 
                 85.0 
                 200.0 
                 3688.0 
               
               
                   
                 Mean 
                 29.3 
                 75.7 
                 200.0 
                 4100.4 
               
               
                   
                 STDER 
                 1.3 
                 4.7 
                 0.0 
                 412.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 28.0 
                 75.0 
                 200.0 
                 3688.0 
               
               
                 YDE043 50 nM 
                 2nd 
                 28.0 
                 70.0 
                 200.0 
                 3688.0 
               
               
                   
                 3rd 
                 30.0 
                 81.0 
                 200.0 
                 4302.5 
               
               
                   
                 Mean 
                 28.7 
                 75.3 
                 200.0 
                 3892.8 
               
               
                   
                 STDER 
                 0.7 
                 3.2 
                 0.0 
                 204.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 30.0 
                 50.0 
                 200.0 
                 4302.5 
               
               
                 YDE043 5 nM 
                 2nd 
                 28.0 
                 67.0 
                 200.0 
                 3688.0 
               
               
                   
                 3rd 
                 27.5 
                 72.0 
                 200.0 
                 3535.8 
               
               
                   
                 Mean 
                 28.5 
                 63.0 
                 200.0 
                 3842.1 
               
               
                   
                 STDER 
                 0.8 
                 6.7 
                 0.0 
                 234.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 28.0 
                 60.0 
                 200.0 
                 3688.0 
               
               
                 YDE043 0.5 nM 
                 2nd 
                 28.0 
                 86.0 
                 200.0 
                 3688.0 
               
               
                   
                 3rd 
                 26.5 
                 64.0 
                 200.0 
                 3233.2 
               
               
                   
                 Mean 
                 27.5 
                 70.0 
                 200.0 
                 3536.4 
               
               
                   
                 STDER 
                 0.5 
                 8.1 
                 0.0 
                 151.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 29.0 
                 75.0 
                 200.0 
                 3994.2 
               
               
                 YDE043 0.05 nM 
                 2nd 
                 29.5 
                 70.0 
                 200.0 
                 4148.1 
               
               
                   
                 3rd 
                 28.0 
                 76.0 
                 200.0 
                 3688.0 
               
               
                   
                 Mean 
                 28.8 
                 73.7 
                 200.0 
                 3943.4 
               
               
                   
                 STDER 
                 0.4 
                 1.9 
                 0.0 
                 135.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 29.0 
                 53.0 
                 200.0 
                 3994.2 
               
               
                 YDE043 0.005 nM 
                 2nd 
                 34.0 
                 69.0 
                 200.0 
                 5555.3 
               
               
                   
                 3rd 
                 36.0 
                 79.0 
                 200.0 
                 6192.0 
               
               
                   
                 Mean 
                 33.0 
                 67.0 
                 200.0 
                 5247.2 
               
               
                   
                 STDER 
                 2.1 
                 7.6 
                 0.0 
                 652.9 
               
               
                   
               
            
           
         
       
     
     Example 9: Investigate the Effects of YY-101, YDE-011 &amp; YDE-043 Compounds on Various Cytokine and Chemokine Release in Human Peripheral Blood Mononuclear Cells (PBMCs) (Study 2) 
     Background and Purpose 
     The objective of this study was to investigate the effects of YY-101, YDE-011 and YDE-043 compound on various cytokine and chemokine release in human peripheral blood mononuclear cells (hPBMCs) stimulated by known stimulants LPS. 
     To achieve this goal, this study was conducted to establish the efficacious dose-response curve of YY-101, YDE-011 and YDE-043 up to 30 cytokine and chemokine release in hPBMC induced by LPS. 
     Materials &amp; Methods 
     Human PBMCs frozen purchased from ATCC in a cryo-preservative were thawed, washed with Hank&#39;s Balanced Salt Solution containing 10% fetal bovine serum, and seeded onto a 24-well plate at a density of 0.5×106 cells/well with culture RPMI media containing 10% fetal bovine serum in growth media in a 24 well plate for 24 hrs and maintained at 37° C. in an atmosphere of 95% air and 5% CO2. At the end of 24 hr culture, cells are pretreated with YY-101, YDE-011 and YDE-043 or reference compound, Xiidra® at various concentrations for 2 h before stimulation. An equal volume of 0.5% DMSO is used as a vehicle control. Then, the cells are treated with LPS. Supernatants from treated cells are harvested at 24 hr post-LPS treatment and placed in 96 well for Luminex cytokine &amp; chemokine profiling using a Luminex 200 multiplex assay. 
     Reagents and Solutions 
     
         
         
           
             Human PBMCs (ATCC, USA) 
             Lipopolysaccharides from  Escherichia coli  (Sigma Aldrich, USA) 
             Human magnetic Luminex assay kit (R&amp;D System, USA) 
             Xiidra® (Shire, USA) 
             Test materials (YY-101, YDE-011 and YDE-043) were stored in a deep freezer (−20° C.). 
           
         
       
    
     Cell Culture 
     Human PBMCs frozen in a cryopreserve was thawed, washed with Hank&#39;s Balanced Salt Solution containing 10% Fetal bovine serum, and seeded onto a 24-well plate at a density of 0.5×10 6  cells/well with culture RPMI media containing 10% Fetal bovine serum in growth media in a 24 well plate for 24 hrs and maintained at 37° C. in an atmosphere of 95% air and 5% CO2. 
     Treatment Human PBMCs were pretreated with YY-101, YDE-011 and YDE-043 or reference compound; Xiidra® at various concentration (ranging from 1 nM to 100 uM) for 2 h before stimulation. An equal volume of 0.5% DMSO was used as a vehicle control. Then, the cells were treated with LPS at 5 ug/ml. The supernatants from treated cells were harvested at 24 h and placed in a 96 well for Luminex cytokine &amp; chemokine profiling using a Luminex 200 multiplex assay (Luminex; R&amp;D system, USA). 
     The main study arm with triplicate wells per group in a 24-well plate:
         1. Blank   2. PBMC: 0.5% DMSO   3. PBMC+LPS (5 ug/ml)   4. PBMC+YY-101 100 uM   5. PBMC+YY-101 10 uM   6. PBMC+YY-101 1 uM   7. PBMC+YY-101 0.1 uM   8. PBMC+YY-101 0.01 uM   9. PBMC+LPS (5 ug/ml)+Xiidra®100 uM   10. PBMC+LPS (5 ug/ml)+Xiidra® 10 uM   11. PBMC+LPS (5 ug/ml)+Xiidra® 1 uM   12. PBMC+LPS (5 ug/ml)+Xiidra®0.1 uM   13. PBMC+LPS (5 ug/ml)+Xiidra®0.01 uM   14. PBMC+LPS (5 ug/ml)+Xiidra®0.001 uM   15. PBMC+LPS (5 ug/ml)+YY-101 100 uM   16. PBMC+LPS (5 ug/ml)+YY-101 10 uM   17. PBMC+LPS (5 ug/ml)+YY-101 1 uM   18. PBMC+LPS (5 ug/ml)+YY-101 0.1 uM   19. PBMC+LPS (5 ug/ml)+YY-101 0.01 uM   20. PBMC+LPS (5 ug/ml)+YY-101 0.001 uM   21. PBMC+LPS (5 ug/ml)+YDE-011 100 uM   22. PBMC+LPS (5 ug/ml)+YDE-011 10 uM   23. PBMC+LPS (5 ug/ml)+YDE-011 1 uM   24. PBMC+LPS (5 ug/ml)+YDE-011 0.1 uM   25. PBMC+LPS (5 ug/ml)+YDE-011 0.01 uM   26. PBMC+LPS (5 ug/ml)+YDE-011 0.001 uM   27. PBMC+LPS (5 ug/ml)+YDE-043 100 uM   28. PBMC+LPS (5 ug/ml)+YDE-043 10 uM   29. PBMC+LPS (5 ug/ml)+YDE-043 1 uM   30. PBMC+LPS (5 ug/ml)+YDE-043 0.1 uM   31. PBMC+LPS (5 ug/ml)+YDE-043 0.01 uM   32. PBMC+LPS (5 ug/ml)+YDE-043 0.001 uM       

     Cell Count 
     The number of PBMCs was measured after 24 hr of treatment. The culture both and trypan blue were diluted 1:1 and 10 ul was added to Countess cell counting chamber (Thermo fisher, USA). The number of PBMCs was measured using a Countess automated cell counter (Thermo fisher, USA). 
     Multiplex Assay 
     Multiplex assay was used to measure 30 cytokines and chemokines. The 1:2, 1:10 and 1:200 diluted cell supernatant and serial dilution of the standard were dispensed into a 96 well plate at 50 ul/well. Then, the pre-mixed cocktail of antibody-coated magnetic beads was dispensed at 50 ul/well and incubated at room temperature for 2 h in a horizontal orbital microplate shaker at 800±500 rpm. The beads were washed using a magnetic device to prevent loss. The biotin-antibody was dispensed in 50 ul of each well and incubated at room temperature for 1 h in a shaker at 800±500 rpm. After washing, streptavidin-PE was added at 50 ul/well and incubated at room temperature for 30 min in a shaker under the same conditions. Finally, after washing, wash buffer (100 ul/well) was added to the plate and incubated in a shaker for 2 min. The Luminex™ 200 setting were set according to the manufacturer&#39;s protocol. The data was calculated with a standard five-parameter logistic nonlinear regression analysis of the data (xPonent software 4.2, USA). The levels of cytokine/chemokine are normalized to the live cell counts (1×10 5  cells/mL) and presented as concentrations per mL (pg/mL or ng/mL). 
     Below is the list of cytokines and chemokines either in 21-plex, 5-plex and two 2-plex (Table 23). 
     
       
         
           
               
               
               
               
             
               
                 TABLE 23 
               
               
                   
               
             
            
               
                 21 plex 
                 1:2 dilution 
                 5 plex 
                 1:10 dilution 
               
               
                   
               
               
                 Human 
                 Cytokine/Chemokine 
                 Human 
                 Cytokine/Chemokine 
               
               
                 1 
                 MCP-1 (CCL2) 
                 1 
                 MIP-1 alpha (CCL3) 
               
               
                 2 
                 RANTES (CCL5) 
                 2 
                 MIP-1 beta (CCL4) 
               
               
                 3 
                 MIP-3alpha (CCL20) 
                 3 
                 IL-1 beta 
               
               
                 4 
                 Fractalkine (CX3CL1) 
                 4 
                 IL-10 
               
               
                 5 
                 Fas-L 
                 5 
                 TNF-alpha 
               
               
                 6 
                 GM-CSF 
               
               
                 7 
                 ICAM-1 
               
               
                 8 
                 IFN-gamma 
               
               
                 9 
                 IL-1 alpha 
               
               
                 10 
                 IL-2 
               
               
                 11 
                 IL-4 
               
               
                 12 
                 IL-5 
               
               
                 13 
                 IL-13 
               
               
                 14 
                 IL-17A 
               
               
                 15 
                 Leptin 
               
               
                 16 
                 MMP-3 
               
               
                 17 
                 MMP-8 
               
               
                 18 
                 MMP-9 
               
               
                 19 
                 RAGE 
               
               
                 20 
                 L-Selectin 
               
               
                 21 
                 VEGF-D 
               
               
                   
               
               
                 2-plex 
                 1:2 dilution 
                 2-plex 
                 1:200 dilution 
               
               
                   
               
               
                 Human 
                 Cytokine/Chemokine list 
                 Human 
                 Cytokine/Chemokine 
               
               
                 1 
                 TIMP-1 
                 1 
                 IL-6 
               
               
                 2 
                 VEGF-A 
                 2 
                 IL-8 (CXCL8) 
               
               
                   
               
            
           
         
       
     
     Compound Delivery &amp; Formulation 
     The concentration of compound was prepared according to the information provided by the client. 
     All compound was serially diluted in from the stock concentrations and the final concentration of DMSO was not exceed 0.5% DMSO. All compounds were prepared on the same day of the treatment. 
     Results 
     Results are shown in  FIGS.  23 - 42   . hPBMCs stimulated by LPS at 5 ug/mL for 24 hrs were co-treated with test and reference compounds with various concentrations and from which cytokine release was measured. Among 30 cytokines and chemokines evaluated, similar to the previous study (NS-Y0119 1st main test), all of the compounds, YY-101, YDE-011 and YDE-043 effectively and potently reduced LPS-induced various pro-inflammatory cytokines and chemokines in human PBMCs at as low as 1 nM. Among the compounds, both YDE-011 and YDE-043 significantly reduced various pro-inflammatory cytokines and chemokines such as TL-6, IL-8, IL-10, TNF-α, IFN-gamma, CCL2, CCL4, CCL5, CCL20, Fas, TIMP-1 more than 50% and CCL-3 over 70%. Interestingly, the compound, YY-101 didn&#39;t affect the basal levels of most of cytokine/chemokine production or the viability of hPBMCs. 
     Human PBMCs were stimulated by LPS at 5 ug/mL for 24 hrs and co-treated with vehicle (0.5% DMSO), test articles and reference compound, Xiidra® at various concentrations (100 uM to 1 nM). After 24 hr treatment, aliquots of hPBMC media were extracted, diluted in 1:2, 1:10 and 1:200, and measured for cytokine levels using a Luminex multiplex system. Additional aliquots were extracted to count the number of total and live PBMCs. 
     hPBMC Cell Counts with or without LPS and Test Articles 
     A range of the total and live cells after 24-hour treatment was between 5.8-13.0×10 5  and 4.6-7.2×10 5  cells per well (1 mL), respectively. The percentage of live over the total cells was between 54-89.5%, of which the majority falls within 7080% range (Table 1). Although some variability among groups exists, when compared to each treatment groups, little or no difference in the total and live cells or viability % was observed in any groups. In other words, none of LPS alone or YY-101 alone, LPS+Xiidra® LPS+YY-101, LPS+YDE-011, or LPS+YDE-043 consistently affected the total and live number of PBMCs up to 100 μM. Neither did the percent viability. 
     Multi-Cytokine Assessment in PBMCs Stimulated by LPS 
     Among 30 cytokines &amp; chemokines evaluated, 15 of 30 were detected within the standard curve range, 5 below the range and 10 were below the level of detection &amp; quantification even though the sample dilution was minimized (Table 2). 
     Among those 15 detectable cytokines/chemokines, similar to the previous study (NS-Y0119 1 st  main test), all of the compounds, YY-101, YDE-011 and YDE-043, effectively and potently reduced LPS-induced various pro-inflammatory cytokines and chemokines in human PBMCs at as low as 1 nM. Among the compounds, both YDE-011 and YDE-043 significantly reduced various pro-inflammatory cytokines and chemokines such as IL-6, IL-8, IL-10, TNF-α, IFN-gamma, CCL2, CCL4, CCL5, CCL20, Fas, TIMP-1 more than 50% and CCL-3 over 70%. YY-101 also significantly reduced LPS-induced various cytokines and chemokines (IL-10, TNF-alpha, CCL4) in human PBMCs at as low as 1 nM. 
     CCL3 is a chemokine ligand 3 known as macrophage inflammatory protein 1-alpha (MIP-1-alpha), which is involved in the acute inflammatory state in the recruitment and activation of leukocytes through binding to the receptors CCR1, CCR4 and CCR5. CCL3 has known to interact with CCL4 and attracts macrophages, monocytes and neutrophils. Interestingly, CCL3 concentrations were significantly increased in Sjogren&#39;s syndrome patients and CCL3 and CCL4 levels correlated significantly with basal tear secretion, tear clearance rate, keratoepitheliopathy score, and goblet cell density. The level correlates with various tear film and ocular surface parameters. (Choi et al., Curr Eye Res. 2012 37:12-7). 
     Although the detection level was low, all of the compounds potently reduced LPS-induced IL2, IL-4 and IL-17A levels in a dose-dependent manner. In contrast, YY-101 also further increased LPS-induced IL-1a, IL-I3, CCL20 and GM-CSF levels at 100.iM, which may due to the YY-101 mediated cellular toxicity. However, it is unlikely as YY-101 at 100.iM didn&#39;t affect any viability or the total number of PBMCs (Table 1). Similar to the previous finding, YY-101, even up to 100.iM didn&#39;t affect the basal levels of cytokine production except TIMP-1. 
     When the reference compound, Xiidra® was tested at higher concentrations than the previous study, it began to reduce some of cytokines induced by LPS. Xiidra® significantly reduced IL-10, CCL3, CCL4, IFN-γ. 
     
       
         
           
               
             
               
                 TABLE 24 
               
             
            
               
                   
               
               
                 PBMC Cell Counts with and without LPS and test articles. 
               
            
           
           
               
               
               
               
            
               
                   
                 Total cell 
                 Live cell 
                 Viability 
               
               
                   
                 (10 5 /ml) 
                 (10 5 /ml) 
                 (%) 
               
               
                   
                   
               
            
           
           
               
               
               
               
            
               
                 PBMC + 0.5% DMSO 
                 8.2 ± 0.4 
                 7.2 ± 0.3 
                 87.3 ± 0.9 
               
               
                 PBMC + LPS(5 ug/ml) 
                 6.8 ± .  
                 5.8 ± 0.2 
                 85.0 ± 0.6 
               
               
                 PBMC + YY101 0.01 uM 
                 5.9 ± 0.4 
                 5.0 ± 0.2 
                 85.0 ± 2.0 
               
               
                 PBMC + YY101 0.1 uM 
                 6.1 ± 0.8 
                 5.1 ± 0.2 
                 84.0 ± 0.0 
               
               
                 PBMC + YY101 1 uM 
                 6.1 ± 0.2 
                 5.5 ± 0.0 
                 89.5 ± 2.5 
               
               
                 PBMC + YY101 10 uM 
                 7.4 ± 0.3 
                 6.3 ± 0.2 
                 86.0 ± 1.0 
               
               
                 PBMC + YY101 100 uM 
                 7.8 ± 0.5 
                 6.7 ± 0.3 
                 86.5 ± 1.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 13.0 ± 3.0 
                 6.9 ± 0.7 
                 54.0 ± 8.0 
               
               
                 Xiidra ® 0.001 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 6.9 ± 0.6 
                 5.5 ± 0.3 
                 80.0 ± 2.0 
               
               
                 Xiidra ® 0.01 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 7.1 ± 0.7 
                 6.2 ± 0.5 
                 86.5 ± 1.5 
               
               
                 Xiidra ® 0.1 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 5.8 ± 0.3 
                 4.6 ± 0.2 
                 80.5 ± 0.5 
               
               
                 Xiidra ® 1 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 6.9 ± 0.2 
                 6.1 ± 0.3 
                 89.0 ± 2.0 
               
               
                 Xiidra ® 10 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 7.5 ± 0.9 
                 6.5 ± 0.7 
                 86.5 ± 1.5 
               
               
                 Xiidra ® 100 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 7.7 ± 0.6 
                 6.5 ± 0.4 
                 84.3 ± 2.6 
               
               
                 YY-101 0.001 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 8.1 ± 0.6 
                 6.2 ± 0.1 
                 77.3 ± 5.0 
               
               
                 YY-101 0.01 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 7.8 ± 0.8 
                 5.9 ± 0.3 
                 75.7 ± 4.7 
               
               
                 YY-101 0.1 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 9.8 ± 0.6 
                 6.5 ± 0.3 
                 66.7 ± 5.8 
               
               
                 YY-101 1 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 9.1 ± 1.1 
                 6.7 ± 0.5 
                 73.7 ± 4.8 
               
               
                 YY-101 10 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 7.8 ± 1.0 
                 5.8 ± 0.4 
                 76.7 ± 5.2 
               
               
                 YY-101 100 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 6.3 ± 0.2 
                 5.4 ± 0.4 
                 85.0 ± 5.1 
               
               
                 YDE-011 0.001 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 7.3 ± 0.4 
                 5.9 ± 0.4 
                 81.0 ± 1.2 
               
               
                 YDE-011 0.01 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 7.1 ± 0.7 
                 5.8 ± 0.5 
                 82.0 ± 4.0 
               
               
                 YDE-011 0.1 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 6.4 ± 0.3 
                 5.0 ± 0.2 
                 79.7 ± 5.8 
               
               
                 YDE-011 1 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 6.0 ± 0.4 
                 5.3 ± 0.3 
                 88.3 ± 1.5 
               
               
                 YDE-011 10 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 8.2 ± 0.4 
                 6.1 ± 0.0 
                 74.3 ± 4.1 
               
               
                 YDE-011 100 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 6.3 ± 0.7 
                 5.0 ± 0.5 
                 79.3 ± 1.5 
               
               
                 YDE-043 0.001 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 6.9 ± 0.4 
                 5.4 ± 0.6 
                 77.3 ± 6.9 
               
               
                 YDE-043 0.01 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 6.6 ± 0.1 
                 5.4 ± 0.2 
                 82.0 ± 4.0 
               
               
                 YDE-043 0.1 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 6.8 ± 0.6 
                 5.3 ± 0.3 
                 78.0 ± 5.0 
               
               
                 YDE-043 1 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 6.4 ± 0.1 
                 5.4 ± 0.1 
                 83.3 ± 3.3 
               
               
                 YDE-043 10 uM 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 7.6 ± 0.6 
                 6.3 ± 0.1 
                 84.5 ± 4.5 
               
               
                 YDE-043 100 uM 
               
               
                   
               
            
           
         
       
     
     Statistical Analysis 
     All values are presented as mean±standard error of mean (SEM). The statistical significance of the results was analyzed using one-way ANOVA with a Bonferroni post hoc. The statistical analyses were performed using the SPSS software (SPSS 22.0, USA). Each compound treated PBMCs was compared to that of the stimulant induced PBMCs. The significant threshold was fixed at 0.05 i.e. p value has to be lower than 0.05 to be significant. 
     Conclusions 
     LPS effectively induced various pro-inflammatory cytokines in human PBMCs and its induction was potently and significantly reduced by all compounds as low as 1 nM; whereas, the reference compound, Xiidra® was not as effective as the compounds. This finding is consistent with Example 8, suggesting that the compounds, especially YDE-011 and YDE043, are more potent and effective immunomodulators in lowering pro-inflammatory cytokine/chemokine productions in human PBMCs when stimulated by an endotoxin, LPS. 
     
       
         
           
               
             
               
                 TABLE 25 
               
             
            
               
                   
               
               
                 A list of cytokines/chemokines and QC results 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Dilution 
                   
               
               
                 No. 
                 Cytokine 
                 factor 
                 Result 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 1 
                 IL-10 
                  1:10 
                 Within standard curve range 
               
               
                 2 
                 IL-8 
                  1:200 
                 Within standard curve range 
               
               
                 3 
                 IL-6 
                  1:200 
                 Within standard curve range 
               
               
                 4 
                 TNF-α 
                  1:10 
                 Within standard curve range 
               
               
                 5 
                 CCL3/MIP-1 α 
                  1:10 
                 Within standard curve range 
               
               
                 6 
                 CCL4/MIP-1 β 
                  1:10 
                 Within standard curve range 
               
               
                 7 
                 CCL5/RANTES 
                 1:2 
                 Within standard curve range 
               
               
                 8 
                 IFN-γ 
                 1:2 
                 Within standard curve range 
               
               
                 9 
                 CCL2/JE/MCP-1 
                 1:2 
                 Within standard curve range 
               
               
                 10 
                 Fas Ligand 
                 1:2 
                 Within standard curve range 
               
               
                 11 
                 CCL20/MIP-3 α 
                 1:2 
                 Within standard curve range 
               
               
                 12 
                 IL-1 α 
                 1:2 
                 Within standard curve range 
               
               
                 13 
                 IL-1 β/IL-1F2 
                  1:10 
                 Within standard curve range 
               
               
                 14 
                 TIMP-1 
                 1:2 
                 Within standard curve range 
               
               
                 15 
                 GM-CSF 
                 1:2 
                 Within standard curve range 
               
               
                 16 
                 IL-3 
                 1:2 
                 Below standard curve range 
               
               
                 17 
                 IL-17A 
                 1:2 
                 Below standard curve range 
               
               
                 18 
                 IL-4 
                 1:2 
                 Below standard curve range 
               
               
                 19 
                 VEGF-A 
                 1:2 
                 Below standard curve range 
               
               
                 20 
                 MMP-9 
                 1:2 
                 Below standard curve range 
               
               
                 21 
                 MMP-3 
                 1:2 
                 BLQ 
               
               
                 22 
                 MMP-8 
                 1:2 
                 BLQ 
               
               
                 23 
                 L-Selectin 
                 1:2 
                 BLQ 
               
               
                 24 
                 RAGE 
                 1:2 
                 BLQ 
               
               
                 25 
                 Fractalkine 
                 1:2 
                 BLQ 
               
               
                 26 
                 IL-5 
                 1:2 
                 BLQ 
               
               
                 27 
                 IL-13 
                 1:2 
                 BLQ 
               
               
                 28 
                 Leptin 
                 1:2 
                 BLQ 
               
               
                 29 
                 ICAM-1 
                 1:2 
                 BLQ 
               
               
                 30 
                 VEGF-D 
                 1:2 
                 BLQ 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 26 
               
             
            
               
                   
               
               
                 Annex 1 - The count of PBMCs raw data 
               
               
                 PBMCs count 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                 Total cell 
                   
                   
               
               
                   
                   
                 count 
                 Live cell 
                 Viability 
               
               
                 Sample 
                   
                 (10{circumflex over ( )}5/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (%) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 PBMC + 0.5% DMSO 
                 1st 
                 8.7 
                 7.5 
                 86.0 
               
               
                   
                 2nd 
                 8.5 
                 7.4 
                 87.0 
               
               
                   
                 3rd 
                 7.5 
                 6.6 
                 89.0 
               
               
                   
                 Mean 
                 8.2 
                 7.2 
                 87.3 
               
               
                   
                 STDER 
                 0.4 
                 0.3 
                 0.9 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 7.3 
                 6.1 
                 84.0 
               
               
                   
                 2nd 
                 6.7 
                 5.8 
                 86.0 
               
               
                   
                 3rd 
                 6.4 
                 5.5 
                 85.0 
               
               
                   
                 Mean 
                 6.8 
                 5.8 
                 85.0 
               
               
                   
                 STDER 
                 0.3 
                 0.2 
                 0.6 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 6.3 
                 5.2 
                 83.0 
               
               
                   
                 2nd 
                 5.5 
                 4.8 
                 87.0 
               
               
                   
                 Mean 
                 5.9 
                 5.0 
                 85.0 
               
               
                   
                 STDER 
                 0.4 
                 0.2 
                 2.0 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 5.2 
                 4.4 
                 84.0 
               
               
                   
                 2nd 
                 6.9 
                 5.8 
                 84.0 
               
               
                   
                 Mean 
                 6.1 
                 5.1 
                 84.0 
               
               
                   
                 STDER 
                 0.8 
                 0.7 
                 0.0 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 5.9 
                 5.5 
                 92.0 
               
               
                   
                 2nd 
                 6.3 
                 5.5 
                 87.0 
               
               
                   
                 Mean 
                 6.1 
                 5.5 
                 89.5 
               
               
                   
                 STDER 
                 0.2 
                 0.0 
                 2.5 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 7.6 
                 6.4 
                 85.0 
               
               
                   
                 2nd 
                 7.1 
                 6.1 
                 87.0 
               
               
                   
                 Mean 
                 7.4 
                 6.3 
                 86.0 
               
               
                   
                 STDER 
                 0.3 
                 0.2 
                 1.0 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 7.3 
                 6.4 
                 88.0 
               
               
                   
                 2nd 
                 8.2 
                 7.0 
                 85.0 
               
               
                   
                 Mean 
                 7.8 
                 6.7 
                 86.5 
               
               
                   
                 STDER 
                 0.5 
                 0.3 
                 1.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 10.0 
                 6.2 
                 62.0 
               
               
                 Xiidra 0.001 uM 
                 2nd 
                 16.0 
                 7.6 
                 46.0 
               
               
                   
                 Mean 
                 13.0 
                 6.9 
                 54.0 
               
               
                   
                 STDER 
                 3.0 
                 0.7 
                 8.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 6.3 
                 5.2 
                 82.0 
               
               
                 Xiidra 0.01 uM 
                 2nd 
                 7.4 
                 5.8 
                 78.0 
               
               
                   
                 Mean 
                 6.9 
                 5.5 
                 80.0 
               
               
                   
                 STDER 
                 0.6 
                 0.3 
                 2.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 7.8 
                 6.6 
                 85.0 
               
               
                 Xiidra 0.1 uM 
                 2nd 
                 6.4 
                 5.7 
                 88.0 
               
               
                   
                 Mean 
                 7.1 
                 6.2 
                 86.5 
               
               
                   
                 STDER 
                 0.7 
                 0.5 
                 1.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 6.0 
                 4.8 
                 81.0 
               
               
                 Xiidra 1 uM 
                 2nd 
                 5.5 
                 4.4 
                 80.0 
               
               
                   
                 Mean 
                 5.8 
                 4.6 
                 80.5 
               
               
                   
                 STDER 
                 0.3 
                 0.2 
                 0.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 6.7 
                 5.8 
                 87.0 
               
               
                 Xiidra 10 uM 
                 2nd 
                 7.1 
                 6.4 
                 91.0 
               
               
                   
                 Mean 
                 6.9 
                 6.1 
                 89.0 
               
               
                   
                 STDER 
                 0.2 
                 0.3 
                 2.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 8.3 
                 7.1 
                 85.0 
               
               
                 Xiidra 100 uM 
                 2nd 
                 6.6 
                 5.8 
                 88.0 
               
               
                   
                 Mean 
                 7.5 
                 6.5 
                 86.5 
               
               
                   
                 STDER 
                 0.9 
                 0.7 
                 1.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 6.7 
                 5.7 
                 84.0 
               
               
                 YY101 0.001 uM 
                 2nd 
                 8.8 
                 7.1 
                 80.0 
               
               
                   
                 3rd 
                 7.5 
                 6.6 
                 89.0 
               
               
                   
                 Mean 
                 7.7 
                 6.5 
                 84.3 
               
               
                   
                 STDER 
                 0.6 
                 0.4 
                 2.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 9.2 
                 6.4 
                 70.0 
               
               
                 YY101 0.01 uM 
                 2nd 
                 8.1 
                 6.0 
                 75.0 
               
               
                   
                 3rd 
                 7.0 
                 6.1 
                 87.0 
               
               
                   
                 Mean 
                 8.1 
                 6.2 
                 77.3 
               
               
                   
                 STDER 
                 0.6 
                 0.1 
                 5.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 9.3 
                 6.2 
                 67.0 
               
               
                 YY101 0.1 uM 
                 2nd 
                 6.7 
                 5.2 
                 77.0 
               
               
                   
                 3rd 
                 7.5 
                 6.2 
                 83.0 
               
               
                   
                 Mean 
                 7.8 
                 5.9 
                 75.7 
               
               
                   
                 STDER 
                 0.8 
                 0.3 
                 4.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 8.8 
                 6.3 
                 72.0 
               
               
                 YY101 1 uM 
                 2nd 
                 9.7 
                 7.1 
                 73.0 
               
               
                   
                 3rd 
                 11.0 
                 6.2 
                 55.0 
               
               
                   
                 Mean 
                 9.8 
                 6.5 
                 66.7 
               
               
                   
                 STDER 
                 0.6 
                 0.3 
                 5.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 7.3 
                 6.0 
                 83.0 
               
               
                 YY101 10 uM 
                 2nd 
                 9.0 
                 6.4 
                 71.0 
               
               
                   
                 3rd 
                 11.0 
                 7.6 
                 67.0 
               
               
                   
                 Mean 
                 9.1 
                 6.7 
                 73.7 
               
               
                   
                 STDER 
                 1.1 
                 0.5 
                 4.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 8.7 
                 6.3 
                 73.0 
               
               
                 YY101 100 uM 
                 2nd 
                 8.8 
                 6.2 
                 70.0 
               
               
                   
                 3rd 
                 5.8 
                 5.0 
                 87.0 
               
               
                   
                 Mean 
                 7.8 
                 5.8 
                 76.7 
               
               
                   
                 STDER 
                 1.0 
                 0.4 
                 5.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 5.9 
                 4.5 
                 75.0 
               
               
                 YDE011 0.001 uM 
                 2nd 
                 6.3 
                 5.8 
                 92.0 
               
               
                   
                 3rd 
                 6.6 
                 5.8 
                 88.0 
               
               
                   
                 Mean 
                 6.3 
                 5.4 
                 85.0 
               
               
                   
                 STDER 
                 0.2 
                 0.4 
                 5.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 7.6 
                 6.3 
                 83.0 
               
               
                 YDE011 0.01 uM 
                 2nd 
                 7.8 
                 6.2 
                 81.0 
               
               
                   
                 3rd 
                 6.4 
                 5.1 
                 79.0 
               
               
                   
                 Mean 
                 7.3 
                 5.9 
                 81.0 
               
               
                   
                 STDER 
                 0.4 
                 0.4 
                 1.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 7.8 
                 6.6 
                 85.0 
               
               
                 YDE011 0.1 uM 
                 2nd 
                 5.7 
                 5.0 
                 87.0 
               
               
                   
                 3rd 
                 7.7 
                 5.7 
                 74.0 
               
               
                   
                 Mean 
                 7.1 
                 5.8 
                 82.0 
               
               
                   
                 STDER 
                 0.7 
                 0.5 
                 4.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 6.2 
                 5.5 
                 89.0 
               
               
                 YDE011 1 uM 
                 2nd 
                 7.0 
                 4.8 
                 69.0 
               
               
                   
                 3rd 
                 5.9 
                 4.8 
                 81.0 
               
               
                   
                 Mean 
                 6.4 
                 5.0 
                 79.7 
               
               
                   
                 STDER 
                 0.3 
                 0.2 
                 5.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 5.9 
                 5.4 
                 91.0 
               
               
                 YDE011 10 uM 
                 2nd 
                 6.7 
                 5.8 
                 86.0 
               
               
                   
                 3rd 
                 5.4 
                 4.7 
                 88.0 
               
               
                   
                 Mean 
                 6.0 
                 5.3 
                 88.3 
               
               
                   
                 STDER 
                 0.4 
                 0.3 
                 1.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 7.6 
                 6.1 
                 81.0 
               
               
                 YDE011 100 uM 
                 2nd 
                 8.1 
                 6.1 
                 75.0 
               
               
                   
                 3rd 
                 9.0 
                 6.0 
                 67.0 
               
               
                   
                 Mean 
                 8.2 
                 6.1 
                 74.3 
               
               
                   
                 STDER 
                 0.4 
                 0.0 
                 4.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 5.6 
                 4.4 
                 79.0 
               
               
                 YDE043 0.001 uM 
                 2nd 
                 7.8 
                 6.0 
                 77.0 
               
               
                   
                 3rd 
                 5.6 
                 4.6 
                 82.0 
               
               
                   
                 Mean 
                 6.3 
                 5.0 
                 79.3 
               
               
                   
                 STDER 
                 0.7 
                 0.5 
                 1.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 6.2 
                 4.5 
                 72.0 
               
               
                 YDE043 0.01 uM 
                 2nd 
                 7.3 
                 6.6 
                 91.0 
               
               
                   
                 3rd 
                 7.2 
                 5.0 
                 69.0 
               
               
                   
                 Mean 
                 6.9 
                 5.4 
                 77.3 
               
               
                   
                 STDER 
                 0.4 
                 0.6 
                 6.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 6.8 
                 5.0 
                 74.0 
               
               
                 YDE043 0.1 uM 
                 2nd 
                 6.4 
                 5.6 
                 87.0 
               
               
                   
                 3rd 
                 6.5 
                 5.6 
                 85.0 
               
               
                   
                 Mean 
                 6.6 
                 5.4 
                 82.0 
               
               
                   
                 STDER 
                 0.1 
                 0.2 
                 4.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 6.9 
                 5.8 
                 83.0 
               
               
                 YDE043 1 uM 
                 2nd 
                 5.7 
                 4.8 
                 83.0 
               
               
                   
                 3rd 
                 7.8 
                 5.3 
                 68.0 
               
               
                   
                 Mean 
                 6.8 
                 5.3 
                 78.0 
               
               
                   
                 STDER 
                 0.6 
                 0.3 
                 5.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 6.4 
                 5.2 
                 80.0 
               
               
                 YDE043 10 uM 
                 2nd 
                 6.7 
                 5.4 
                 80.0 
               
               
                   
                 3rd 
                 6.2 
                 5.6 
                 90.0 
               
               
                   
                 Mean 
                 6.4 
                 5.4 
                 83.3 
               
               
                   
                 STDER 
                 0.1 
                 0.1 
                 3.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 7.0 
                 6.2 
                 89.0 
               
               
                 YDE043 100 uM 
                 2nd 
                 8.1 
                 6.4 
                 80.0 
               
               
                   
                 Mean 
                 7.6 
                 6.3 
                 84.5 
               
               
                   
                 STDER 
                 0.6 
                 0.1 
                 4.5 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 27 
               
             
            
               
                   
               
               
                 Annex 2-The main study raw data 
               
               
                 Bead name : IL-10 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 −0.3 
                 10.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.3 
                 10.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 10.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 −0.3 
                 10.0 
                   
                 7.5 
                 0.0 
               
               
                   
                 2nd 
                 −0.3 
                 10.0 
                   
                 7.4 
                 0.0 
               
               
                   
                 3rd 
                 −0.3 
                 10.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 Mean 
                 −0.3 
                 10.0 
                 #DIV/0! 
                 7.2 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 503.8 
                 10.0 
                 743.5 
                 6.1 
                 121.9 
               
               
                   
                 2nd 
                 445.8 
                 10.0 
                 670.5 
                 5.8 
                 115.6 
               
               
                   
                 3rd 
                 467.8 
                 10.0 
                 698.3 
                 5.5 
                 127.0 
               
               
                   
                 Mean 
                 472.4 
                 10.0 
                 704.1 
                 5.8 
                 121.5 
               
               
                   
                 STDER 
                 16.9 
                 0.0 
                 21.3 
                 0.2 
                 3.3 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 0.8 
                 10.0 
                   
                 5.2 
                 0.0 
               
               
                   
                 2nd 
                 1.8 
                 10.0 
                 4.3 
                 4.8 
                 0.9 
               
               
                   
                 Mean 
                 1.3 
                 10.0 
                 4.3 
                 5.0 
                 0.5 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 0.2 
                 0.5 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 0.8 
                 10.0 
                   
                 4.4 
                 0.0 
               
               
                   
                 2nd 
                 1.8 
                 10.0 
                 4.3 
                 5.8 
                 0.7 
               
               
                   
                 Mean 
                 1.3 
                 10.0 
                 4.3 
                 5.1 
                 0.4 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 0.7 
                 0.4 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 0.8 
                 10.0 
                   
                 5.5 
                 0.0 
               
               
                   
                 2nd 
                 0.8 
                 10.0 
                   
                 5.5 
                 0.0 
               
               
                   
                 Mean 
                 0.8 
                 10.0 
                 #DIV/0! 
                 5.5 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.0 
                 0.0 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 7.8 
                 10.0 
                 23.2 
                 6.4 
                 3.6 
               
               
                   
                 2nd 
                 1.3 
                 10.0 
                 2.0 
                 6.1 
                 0.3 
               
               
                   
                 Mean 
                 4.5 
                 10.0 
                 12.6 
                 6.3 
                 2.0 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 10.6 
                 0.2 
                 1.6 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 2.8 
                 10.0 
                 8.2 
                 6.4 
                 1.3 
               
               
                   
                 2nd 
                 −0.3 
                 10.0 
                   
                 7.0 
                 0.0 
               
               
                   
                 Mean 
                 1.3 
                 10.0 
                 8.2 
                 6.7 
                 0.6 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.6 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 319.8 
                 10.0 
                 507.4 
                 6.2 
                 81.8 
               
               
                 Xiidra 0.001 uM 
                 2nd 
                 310.3 
                 10.0 
                 494.8 
                 7.6 
                 65.1 
               
               
                   
                 Mean 
                 315.0 
                 10.0 
                 501.1 
                 6.9 
                 73.5 
               
               
                   
                 STDER 
                 4.8 
                 0.0 
                 6.3 
                 0.7 
                 8.4 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 298.8 
                 10.0 
                 479.5 
                 5.2 
                 92.2 
               
               
                 Xiidra 0.01 uM 
                 2nd 
                 327.3 
                 10.0 
                 517.4 
                 5.8 
                 89.2 
               
               
                   
                 Mean 
                 313.0 
                 10.0 
                 498.4 
                 5.5 
                 90.7 
               
               
                   
                 STDER 
                 14.3 
                 0.0 
                 18.9 
                 0.3 
                 1.5 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 257.3 
                 10.0 
                 423.5 
                 6.6 
                 64.2 
               
               
                 Xiidra 0.1 uM 
                 2nd 
                 223.3 
                 10.0 
                 376.6 
                 5.7 
                 66.1 
               
               
                   
                 Mean 
                 240.3 
                 10.0 
                 400.0 
                 6.2 
                 65.1 
               
               
                   
                 STDER 
                 17.0 
                 0.0 
                 23.4 
                 0.5 
                 1.0 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 305.8 
                 10.0 
                 488.8 
                 4.8 
                 101.8 
               
               
                 Xiidra 1 uM 
                 2nd 
                 219.3 
                 10.0 
                 371.0 
                 4.4 
                 84.3 
               
               
                   
                 Mean 
                 262.5 
                 10.0 
                 429.9 
                 4.6 
                 93.1 
               
               
                   
                 STDER 
                 43.3 
                 0.0 
                 58.9 
                 0.2 
                 8.8 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 311.8 
                 10.0 
                 496.8 
                 5.8 
                 85.7 
               
               
                 Xiidra 10 uM 
                 2nd 
                 335.3 
                 10.0 
                 527.9 
                 6.4 
                 82.5 
               
               
                   
                 Mean 
                 323.5 
                 10.0 
                 512.4 
                 6.1 
                 84.1 
               
               
                   
                 STDER 
                 11.8 
                 0.0 
                 15.5 
                 0.3 
                 1.6 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 329.8 
                 10.0 
                 520.7 
                 7.1 
                 73.3 
               
               
                 Xiidra 100 uM 
                 2nd 
                 318.8 
                 10.0 
                 506.1 
                 5.8 
                 87.3 
               
               
                   
                 Mean 
                 324.3 
                 10.0 
                 513.4 
                 6.5 
                 80.3 
               
               
                   
                 STDER 
                 5.5 
                 0.0 
                 7.3 
                 0.7 
                 7.0 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 315.3 
                 10.0 
                 501.5 
                 5.7 
                 88.0 
               
               
                 YY101 0.001 uM 
                 2nd 
                 305.8 
                 10.0 
                 488.8 
                 7.1 
                 68.9 
               
               
                   
                 3rd 
                 275.3 
                 10.0 
                 447.9 
                 6.6 
                 67.9 
               
               
                   
                 Mean 
                 298.8 
                 10.0 
                 479.4 
                 6.5 
                 74.9 
               
               
                   
                 STDER 
                 12.1 
                 0.0 
                 16.2 
                 0.4 
                 6.5 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 308.8 
                 10.0 
                 492.8 
                 6.4 
                 77.0 
               
               
                 YY101 0.01 uM 
                 2nd 
                 313.8 
                 10.0 
                 499.5 
                 6.0 
                 83.2 
               
               
                   
                 3rd 
                 319.8 
                 10.0 
                 507.4 
                 6.1 
                 83.2 
               
               
                   
                 Mean 
                 314.1 
                 10.0 
                 499.9 
                 6.2 
                 81.1 
               
               
                   
                 STDER 
                 3.2 
                 0.0 
                 4.2 
                 0.1 
                 2.1 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 259.8 
                 10.0 
                 426.9 
                 6.2 
                 68.9 
               
               
                 YY101 0.1 uM 
                 2nd 
                 283.8 
                 10.0 
                 459.4 
                 5.2 
                 88.3 
               
               
                   
                 3rd 
                 277.3 
                 10.0 
                 450.6 
                 6.2 
                 72.7 
               
               
                   
                 Mean 
                 273.6 
                 10.0 
                 445.6 
                 5.9 
                 76.6 
               
               
                   
                 STDER 
                 7.2 
                 0.0 
                 9.7 
                 0.3 
                 6.0 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 357.3 
                 10.0 
                 556.7 
                 6.3 
                 88.4 
               
               
                 YY101 1 uM 
                 2nd 
                 302.8 
                 10.0 
                 484.8 
                 7.1 
                 68.3 
               
               
                   
                 3rd 
                 327.8 
                 10.0 
                 518.0 
                 6.2 
                 83.6 
               
               
                   
                 Mean 
                 329.3 
                 10.0 
                 519.9 
                 6.5 
                 80.1 
               
               
                   
                 STDER 
                 15.8 
                 0.0 
                 20.8 
                 0.3 
                 6.1 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 287.8 
                 10.0 
                 464.8 
                 6.0 
                 77.5 
               
               
                 YY101 10 uM 
                 2nd 
                 342.3 
                 10.0 
                 537.1 
                 6.4 
                 83.9 
               
               
                   
                 3rd 
                 255.8 
                 10.0 
                 421.4 
                 7.6 
                 55.4 
               
               
                   
                 Mean 
                 295.3 
                 10.0 
                 474.4 
                 6.7 
                 72.3 
               
               
                   
                 STDER 
                 25.3 
                 0.0 
                 33.7 
                 0.5 
                 8.6 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 86.8 
                 10.0 
                 173.5 
                 6.3 
                 27.5 
               
               
                 YY101 100 uM 
                 2nd 
                 83.8 
                 10.0 
                 168.6 
                 6.2 
                 27.2 
               
               
                   
                 3rd 
                 87.8 
                 10.0 
                 175.1 
                 5.0 
                 35.0 
               
               
                   
                 Mean 
                 86.1 
                 10.0 
                 172.4 
                 5.8 
                 29.9 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 2.0 
                 0.4 
                 2.6 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 163.3 
                 10.0 
                 291.0 
                 4.5 
                 64.7 
               
               
                 YDE011 0.001 uM 
                 2nd 
                 179.3 
                 10.0 
                 314.2 
                 5.8 
                 54.2 
               
               
                   
                 3rd 
                 173.8 
                 10.0 
                 306.3 
                 5.8 
                 52.8 
               
               
                   
                 Mean 
                 172.1 
                 10.0 
                 303.8 
                 5.4 
                 57.2 
               
               
                   
                 STDER 
                 4.7 
                 0.0 
                 6.8 
                 0.4 
                 3.7 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 192.8 
                 10.0 
                 333.6 
                 6.3 
                 52.9 
               
               
                 YDE011 0.01 uM 
                 2nd 
                 186.8 
                 10.0 
                 325.0 
                 6.2 
                 52.4 
               
               
                   
                 3rd 
                 166.3 
                 10.0 
                 295.4 
                 5.1 
                 57.9 
               
               
                   
                 Mean 
                 181.9 
                 10.0 
                 318.0 
                 5.9 
                 54.4 
               
               
                   
                 STDER 
                 8.0 
                 0.0 
                 11.6 
                 0.4 
                 1.7 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 168.8 
                 10.0 
                 299.0 
                 6.6 
                 45.3 
               
               
                 YDE011 0.1 uM 
                 2nd 
                 181.8 
                 10.0 
                 317.8 
                 5.0 
                 63.6 
               
               
                   
                 3rd 
                 176.3 
                 10.0 
                 309.9 
                 5.7 
                 54.4 
               
               
                   
                 Mean 
                 175.6 
                 10.0 
                 308.9 
                 5.8 
                 54.4 
               
               
                   
                 STDER 
                 3.8 
                 0.0 
                 5.5 
                 0.5 
                 5.3 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 179.3 
                 10.0 
                 314.2 
                 5.5 
                 57.1 
               
               
                 YDE011 1 uM 
                 2nd 
                 182.8 
                 10.0 
                 319.3 
                 4.8 
                 66.5 
               
               
                   
                 3rd 
                 176.8 
                 10.0 
                 310.6 
                 4.8 
                 64.7 
               
               
                   
                 Mean 
                 179.6 
                 10.0 
                 314.7 
                 5.0 
                 62.8 
               
               
                   
                 STDER 
                 1.7 
                 0.0 
                 2.5 
                 0.2 
                 2.9 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 168.8 
                 10.0 
                 299.0 
                 5.4 
                 55.4 
               
               
                 YDE011 10 uM 
                 2nd 
                 166.3 
                 10.0 
                 295.4 
                 5.8 
                 50.9 
               
               
                   
                 3rd 
                 174.8 
                 10.0 
                 307.7 
                 4.7 
                 65.5 
               
               
                   
                 Mean 
                 169.9 
                 10.0 
                 300.7 
                 5.3 
                 57.3 
               
               
                   
                 STDER 
                 2.5 
                 0.0 
                 3.7 
                 0.3 
                 4.3 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 43.8 
                 10.0 
                 99.4 
                 6.1 
                 16.3 
               
               
                 YDE011 100 uM 
                 2nd 
                 51.8 
                 10.0 
                 113.9 
                 6.1 
                 18.7 
               
               
                   
                 3rd 
                 55.8 
                 10.0 
                 121.1 
                 6.0 
                 20.2 
               
               
                   
                 Mean 
                 50.4 
                 10.0 
                 111.4 
                 6.1 
                 18.4 
               
               
                   
                 STDER 
                 3.5 
                 0.0 
                 6.4 
                 0.0 
                 1.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 194.3 
                 10.0 
                 335.7 
                 4.4 
                 76.3 
               
               
                   
                 2nd 
                 168.3 
                 10.0 
                 298.3 
                 6.0 
                 49.7 
               
               
                   
                 3rd 
                 157.8 
                 10.0 
                 282.9 
                 4.6 
                 61.5 
               
               
                   
                 Mean 
                 173.4 
                 10.0 
                 305.6 
                 5.0 
                 62.5 
               
               
                   
                 STDER 
                 10.8 
                 0.0 
                 15.7 
                 0.5 
                 7.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 200.8 
                 10.0 
                 344.9 
                 4.5 
                 76.7 
               
               
                   
                 2nd 
                 213.3 
                 10.0 
                 362.6 
                 6.6 
                 54.9 
               
               
                   
                 3rd 
                 188.3 
                 10.0 
                 327.1 
                 5.0 
                 65.4 
               
               
                   
                 Mean 
                 200.8 
                 10.0 
                 344.9 
                 5.4 
                 65.7 
               
               
                   
                 STDER 
                 7.2 
                 0.0 
                 10.2 
                 0.6 
                 6.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 190.3 
                 10.0 
                 330.0 
                 5.0 
                 66.0 
               
               
                   
                 2nd 
                 178.3 
                 10.0 
                 312.8 
                 5.6 
                 55.9 
               
               
                   
                 3rd 
                 186.3 
                 10.0 
                 324.3 
                 5.6 
                 57.9 
               
               
                   
                 Mean 
                 184.9 
                 10.0 
                 322.4 
                 5.4 
                 59.9 
               
               
                   
                 STDER 
                 3.5 
                 0.0 
                 5.1 
                 0.2 
                 3.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 189.3 
                 10.0 
                 328.6 
                 5.8 
                 56.7 
               
               
                   
                 2nd 
                 178.3 
                 10.0 
                 312.8 
                 4.8 
                 65.2 
               
               
                   
                 3rd 
                 198.3 
                 10.0 
                 341.4 
                 5.3 
                 64.4 
               
               
                   
                 Mean 
                 188.6 
                 10.0 
                 327.6 
                 5.3 
                 62.1 
               
               
                   
                 STDER 
                 5.8 
                 0.0 
                 8.3 
                 0.3 
                 2.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 182.3 
                 10.0 
                 318.5 
                 5.2 
                 61.3 
               
               
                   
                 2nd 
                 153.8 
                 10.0 
                 277.0 
                 5.4 
                 51.3 
               
               
                   
                 3rd 
                 167.8 
                 10.0 
                 297.5 
                 5.6 
                 53.1 
               
               
                   
                 Mean 
                 167.9 
                 10.0 
                 297.7 
                 5.4 
                 55.2 
               
               
                   
                 STDER 
                 8.2 
                 0.0 
                 12.0 
                 0.1 
                 3.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 47.8 
                 10.0 
                 106.7 
                 6.2 
                 17.2 
               
               
                   
                 2nd 
                 50.8 
                 10.0 
                 112.1 
                 6.4 
                 17.5 
               
               
                   
                 Mean 
                 49.3 
                 10.0 
                 109.4 
                 6.3 
                 17.4 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 2.7 
                 0.1 
                 0.2 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 28 
               
             
            
               
                   
               
               
                 Bead name : IL-8 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 −0.5 
                 200.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.5 
                 200.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 200.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 68.0 
                 200.0 
                 2052.8 
                 7.5 
                 273.7 
               
               
                   
                 2nd 
                 70.5 
                 200.0 
                 2126.4 
                 7.4 
                 287.4 
               
               
                   
                 3rd 
                 89.0 
                 200.0 
                 2671.9 
                 6.6 
                 404.8 
               
               
                   
                 Mean 
                 75.8 
                 200.0 
                 2283.7 
                 7.2 
                 322.0 
               
               
                   
                 STDER 
                 6.6 
                 0.0 
                 195.3 
                 0.3 
                 41.6 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 1590.5 
                 200.0 
                 48853.0 
                 6.1 
                 8008.7 
               
               
                   
                 2nd 
                 1546.5 
                 200.0 
                 47437.7 
                 5.8 
                 8178.9 
               
               
                   
                 3rd 
                 1575.5 
                 200.0 
                 48370.0 
                 5.5 
                 8794.5 
               
               
                   
                 Mean 
                 1570.8 
                 200.0 
                 48220.2 
                 5.8 
                 8327.4 
               
               
                   
                 STDER 
                 12.9 
                 0.0 
                 415.4 
                 0.2 
                 238.7 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 95.5 
                 200.0 
                 2863.8 
                 5.2 
                 550.7 
               
               
                   
                 2nd 
                 230.0 
                 200.0 
                 6854.4 
                 4.8 
                 1428.0 
               
               
                   
                 Mean 
                 162.8 
                 200.0 
                 4859.1 
                 5.0 
                 989.4 
               
               
                   
                 STDER 
                 67.3 
                 0.0 
                 1995.3 
                 0.2 
                 438.6 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 90.0 
                 200.0 
                 2701.4 
                 4.4 
                 614.0 
               
               
                   
                 2nd 
                 205.5 
                 200.0 
                 6125.1 
                 5.8 
                 1056.0 
               
               
                   
                 Mean 
                 147.8 
                 200.0 
                 4413.2 
                 5.1 
                 835.0 
               
               
                   
                 STDER 
                 57.8 
                 0.0 
                 1711.8 
                 0.7 
                 221.0 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 149.5 
                 200.0 
                 4461.8 
                 5.5 
                 811.2 
               
               
                   
                 2nd 
                 157.5 
                 200.0 
                 4699.1 
                 5.5 
                 854.4 
               
               
                   
                 Mean 
                 153.5 
                 200.0 
                 4580.5 
                 5.5 
                 832.8 
               
               
                   
                 STDER 
                 4.0 
                 0.0 
                 118.6 
                 0.0 
                 21.6 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 1227.5 
                 200.0 
                 37311.2 
                 6.4 
                 5829.9 
               
               
                   
                 2nd 
                 166.5 
                 200.0 
                 4966.2 
                 6.1 
                 814.1 
               
               
                   
                 Mean 
                 697.0 
                 200.0 
                 21138.7 
                 6.3 
                 3322.0 
               
               
                   
                 STDER 
                 530.5 
                 0.0 
                 16172.5 
                 0.2 
                 2507.9 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 664.5 
                 200.0 
                 19929.7 
                 6.4 
                 3114.0 
               
               
                   
                 2nd 
                 358.5 
                 200.0 
                 10693.9 
                 7.0 
                 1527.7 
               
               
                   
                 Mean 
                 511.5 
                 200.0 
                 15311.8 
                 6.7 
                 2320.9 
               
               
                   
                 STDER 
                 153.0 
                 0.0 
                 4617.9 
                 0.3 
                 793.2 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1631.5 
                 200.0 
                 50176.1 
                 6.2 
                 8092.9 
               
               
                 Xiidra 0.001 uM 
                 2nd 
                 1424.5 
                 200.0 
                 43537.7 
                 7.6 
                 5728.6 
               
               
                   
                 Mean 
                 1528.0 
                 200.0 
                 46856.9 
                 6.9 
                 6910.8 
               
               
                   
                 STDER 
                 103.5 
                 0.0 
                 3319.2 
                 0.7 
                 1182.1 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1533.0 
                 200.0 
                 47004.4 
                 5.2 
                 9039.3 
               
               
                 Xiidra 0.01 uM 
                 2nd 
                 1638.0 
                 200.0 
                 50386.3 
                 5.8 
                 8687.3 
               
               
                   
                 Mean 
                 1585.5 
                 200.0 
                 48695.3 
                 5.5 
                 8863.3 
               
               
                   
                 STDER 
                 52.5 
                 0.0 
                 1691.0 
                 0.3 
                 176.0 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1396.5 
                 200.0 
                 42647.5 
                 6.6 
                 6461.7 
               
               
                 Xiidra 0.1 uM 
                 2nd 
                 1433.5 
                 200.0 
                 43824.2 
                 5.7 
                 7688.5 
               
               
                   
                 Mean 
                 1415.0 
                 200.0 
                 43235.9 
                 6.2 
                 7075.1 
               
               
                   
                 STDER 
                 18.5 
                 0.0 
                 588.4 
                 0.5 
                 613.4 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1382.5 
                 200.0 
                 42203.1 
                 4.8 
                 8792.3 
               
               
                 Xiidra 1 uM 
                 2nd 
                 1548.0 
                 200.0 
                 47485.8 
                 4.4 
                 10792.2 
               
               
                   
                 Mean 
                 1465.3 
                 200.0 
                 44844.5 
                 4.6 
                 9792.3 
               
               
                   
                 STDER 
                 82.8 
                 0.0 
                 2641.4 
                 0.2 
                 1000.0 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1418.5 
                 200.0 
                 43346.8 
                 5.8 
                 7473.6 
               
               
                 Xiidra 10 uM 
                 2nd 
                 1370.5 
                 200.0 
                 41822.5 
                 6.4 
                 6534.8 
               
               
                   
                 Mean 
                 1394.5 
                 200.0 
                 42584.6 
                 6.1 
                 7004.2 
               
               
                   
                 STDER 
                 24.0 
                 0.0 
                 762.2 
                 0.3 
                 469.4 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1356.0 
                 200.0 
                 41363.0 
                 7.1 
                 5825.8 
               
               
                 Xiidra 100 uM 
                 2nd 
                 1346.5 
                 200.0 
                 41062.3 
                 5.8 
                 7079.7 
               
               
                   
                 Mean 
                 1351.3 
                 200.0 
                 41212.6 
                 6.5 
                 6452.7 
               
               
                   
                 STDER 
                 4.8 
                 0.0 
                 150.4 
                 0.7 
                 627.0 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1479.5 
                 200.0 
                 45291.5 
                 5.7 
                 7945.9 
               
               
                 YY101 0.001 uM 
                 2nd 
                 1465.5 
                 200.0 
                 44844.4 
                 7.1 
                 6316.1 
               
               
                   
                 3rd 
                 1677.0 
                 200.0 
                 51649.5 
                 6.6 
                 7825.7 
               
               
                   
                 Mean 
                 1540.7 
                 200.0 
                 47261.8 
                 6.5 
                 7362.6 
               
               
                   
                 STDER 
                 68.3 
                 0.0 
                 2197.6 
                 0.4 
                 524.4 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1486.0 
                 200.0 
                 45499.3 
                 6.4 
                 7109.3 
               
               
                 YY101 0.01 uM 
                 2nd 
                 1465.5 
                 200.0 
                 44844.4 
                 6.0 
                 7474.1 
               
               
                   
                 3rd 
                 1515.5 
                 200.0 
                 46443.3 
                 6.1 
                 7613.7 
               
               
                   
                 Mean 
                 1489.0 
                 200.0 
                 45595.7 
                 6.2 
                 7399.0 
               
               
                   
                 STDER 
                 14.5 
                 0.0 
                 464.1 
                 0.1 
                 150.4 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1353.5 
                 200.0 
                 41283.9 
                 6.2 
                 6658.7 
               
               
                 YY101 0.1 uM 
                 2nd 
                 1407.5 
                 200.0 
                 42997.0 
                 5.2 
                 8268.7 
               
               
                   
                 3rd 
                 1359.5 
                 200.0 
                 41473.9 
                 6.2 
                 6689.3 
               
               
                   
                 Mean 
                 1373.5 
                 200.0 
                 41918.3 
                 5.9 
                 7205.6 
               
               
                   
                 STDER 
                 17.1 
                 0.0 
                 542.2 
                 0.3 
                 531.6 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1476.5 
                 200.0 
                 45195.7 
                 6.3 
                 7173.9 
               
               
                 YY101 1 uM 
                 2nd 
                 1478.5 
                 200.0 
                 45259.6 
                 7.1 
                 6374.6 
               
               
                   
                 3rd 
                 1560.0 
                 200.0 
                 47871.4 
                 6.2 
                 7721.2 
               
               
                   
                 Mean 
                 1505.0 
                 200.0 
                 46108.9 
                 6.5 
                 7089.9 
               
               
                   
                 STDER 
                 27.5 
                 0.0 
                 881.4 
                 0.3 
                 391.0 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1511.5 
                 200.0 
                 46315.2 
                 6.0 
                 7719.2 
               
               
                 YY101 10 uM 
                 2nd 
                 1649.0 
                 200.0 
                 50742.2 
                 6.4 
                 7928.5 
               
               
                   
                 3rd 
                 1407.0 
                 200.0 
                 42981.1 
                 7.6 
                 5655.4 
               
               
                   
                 Mean 
                 1522.5 
                 200.0 
                 46679.5 
                 6.7 
                 7101.0 
               
               
                   
                 STDER 
                 70.1 
                 0.0 
                 2247.8 
                 0.5 
                 725.3 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 1574.5 
                 200.0 
                 48337.8 
                 6.3 
                 7672.7 
               
               
                 YY101 100 uM 
                 2nd 
                 1532.5 
                 200.0 
                 46988.3 
                 6.2 
                 7578.8 
               
               
                   
                 3rd 
                 1444.5 
                 200.0 
                 44174.7 
                 5.0 
                 8834.9 
               
               
                   
                 Mean 
                 1517.2 
                 200.0 
                 46500.3 
                 5.8 
                 8028.8 
               
               
                   
                 STDER 
                 38.3 
                 0.0 
                 1226.3 
                 0.4 
                 404.0 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 861.5 
                 200.0 
                 25949.1 
                 4.5 
                 5766.5 
               
               
                 YDE011 0.001 uM 
                 2nd 
                 838.5 
                 200.0 
                 25243.2 
                 5.8 
                 4352.3 
               
               
                   
                 3rd 
                 906.5 
                 200.0 
                 27333.0 
                 5.8 
                 4712.6 
               
               
                   
                 Mean 
                 868.8 
                 200.0 
                 26175.1 
                 5.4 
                 4943.8 
               
               
                   
                 STDER 
                 20.0 
                 0.0 
                 613.8 
                 0.4 
                 424.3 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 906.0 
                 200.0 
                 27317.6 
                 6.3 
                 4336.1 
               
               
                 YDE011 0.01 uM 
                 2nd 
                 964.0 
                 200.0 
                 29106.4 
                 6.2 
                 4694.6 
               
               
                   
                 3rd 
                 900.5 
                 200.0 
                 27148.3 
                 5.1 
                 5323.2 
               
               
                   
                 Mean 
                 923.5 
                 200.0 
                 27857.4 
                 5.9 
                 4784.6 
               
               
                   
                 STDER 
                 20.3 
                 0.0 
                 626.4 
                 0.4 
                 288.5 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 852.5 
                 200.0 
                 25672.8 
                 6.6 
                 3889.8 
               
               
                 YDE011 0.1 uM 
                 2nd 
                 870.5 
                 200.0 
                 26225.6 
                 5.0 
                 5245.1 
               
               
                   
                 3rd 
                 853.5 
                 200.0 
                 25703.5 
                 5.7 
                 4509.4 
               
               
                   
                 Mean 
                 858.8 
                 200.0 
                 25867.3 
                 5.8 
                 4548.1 
               
               
                   
                 STDER 
                 5.8 
                 0.0 
                 179.4 
                 0.5 
                 391.7 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 872.0 
                 200.0 
                 26271.7 
                 5.5 
                 4776.7 
               
               
                 YDE011 1 uM 
                 2nd 
                 875.5 
                 200.0 
                 26379.3 
                 4.8 
                 5495.7 
               
               
                   
                 3rd 
                 805.5 
                 200.0 
                 24231.7 
                 4.8 
                 5048.3 
               
               
                   
                 Mean 
                 851.0 
                 200.0 
                 25627.6 
                 5.0 
                 5106.9 
               
               
                   
                 STDER 
                 22.8 
                 0.0 
                 698.6 
                 0.2 
                 209.6 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 851.0 
                 200.0 
                 25626.7 
                 5.4 
                 4745.7 
               
               
                 YDE011 10 uM 
                 2nd 
                 841.5 
                 200.0 
                 25335.2 
                 5.8 
                 4368.1 
               
               
                   
                 3rd 
                 809.5 
                 200.0 
                 24354.2 
                 4.7 
                 5181.8 
               
               
                   
                 Mean 
                 834.0 
                 200.0 
                 25105.4 
                 5.3 
                 4765.2 
               
               
                   
                 STDER 
                 12.6 
                 0.0 
                 384.9 
                 0.3 
                 235.1 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 918.5 
                 200.0 
                 27702.6 
                 6.1 
                 4541.4 
               
               
                 YDE011 100 uM 
                 2nd 
                 881.5 
                 200.0 
                 26563.8 
                 6.1 
                 4354.7 
               
               
                   
                 3rd 
                 793.0 
                 200.0 
                 23849.1 
                 6.0 
                 3974.8 
               
               
                   
                 Mean 
                 864.3 
                 200.0 
                 26038.5 
                 6.1 
                 4290.3 
               
               
                   
                 STDER 
                 37.2 
                 0.0 
                 1143.0 
                 0.0 
                 166.7 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 841.0 
                 200.0 
                 25319.8 
                 4.4 
                 5754.5 
               
               
                 YDE043 0.001 uM 
                 2nd 
                 932.5 
                 200.0 
                 28134.2 
                 6.0 
                 4689.0 
               
               
                   
                 3rd 
                 973.0 
                 200.0 
                 29384.5 
                 4.6 
                 6387.9 
               
               
                   
                 Mean 
                 915.5 
                 200.0 
                 27612.8 
                 5.0 
                 5610.5 
               
               
                   
                 STDER 
                 39.0 
                 0.0 
                 1202.0 
                 0.5 
                 495.7 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 928.5 
                 200.0 
                 28010.8 
                 4.5 
                 6224.6 
               
               
                 YDE043 0.01 uM 
                 2nd 
                 897.5 
                 200.0 
                 27056.0 
                 6.6 
                 4099.4 
               
               
                   
                 3rd 
                 939.5 
                 200.0 
                 28350.1 
                 5.0 
                 5670.0 
               
               
                   
                 Mean 
                 921.8 
                 200.0 
                 27805.6 
                 5.4 
                 5331.3 
               
               
                   
                 STDER 
                 12.6 
                 0.0 
                 387.4 
                 0.6 
                 636.4 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 912.0 
                 200.0 
                 27502.4 
                 5.0 
                 5500.5 
               
               
                 YDE043 0.1 uM 
                 2nd 
                 822.0 
                 200.0 
                 24737.2 
                 5.6 
                 4417.4 
               
               
                   
                 3rd 
                 926.5 
                 200.0 
                 27949.2 
                 5.6 
                 4990.9 
               
               
                   
                 Mean 
                 886.8 
                 200.0 
                 26729.6 
                 5.4 
                 4969.6 
               
               
                   
                 STDER 
                 32.7 
                 0.0 
                 1004.5 
                 0.2 
                 312.9 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 912.5 
                 200.0 
                 27517.8 
                 5.8 
                 4744.4 
               
               
                 YDE043 1 uM 
                 2nd 
                 765.5 
                 200.0 
                 23008.1 
                 4.8 
                 4793.4 
               
               
                   
                 3rd 
                 954.0 
                 200.0 
                 28797.5 
                 5.3 
                 5433.5 
               
               
                   
                 Mean 
                 877.3 
                 200.0 
                 26441.2 
                 5.3 
                 4990.4 
               
               
                   
                 STDER 
                 57.2 
                 0.0 
                 1755.8 
                 0.3 
                 222.0 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 960.5 
                 200.0 
                 28998.3 
                 5.2 
                 5576.6 
               
               
                 YDE043 10 uM 
                 2nd 
                 1141.5 
                 200.0 
                 34618.6 
                 5.4 
                 6410.8 
               
               
                   
                 3rd 
                 812.5 
                 200.0 
                 24446.1 
                 5.6 
                 4365.4 
               
               
                   
                 Mean 
                 971.5 
                 200.0 
                 29354.3 
                 5.4 
                 5450.9 
               
               
                   
                 STDER 
                 95.1 
                 0.0 
                 2941.9 
                 0.1 
                 593.8 
               
               
                 PBMC + LPS(5 ug/ml) +  
                 1st 
                 895.5 
                 200.0 
                 26994.4 
                 6.2 
                 4353.9 
               
               
                 YDE043 100 uM 
                 2nd 
                 798.5 
                 200.0 
                 24017.4 
                 6.4 
                 3752.7 
               
               
                   
                 Mean 
                 847.0 
                 200.0 
                 25505.9 
                 6.3 
                 4053.3 
               
               
                   
                 STDER 
                 48.5 
                 0.0 
                 1488.5 
                 0.1 
                 300.6 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 29 
               
             
            
               
                   
               
               
                 Bead name: IL-6 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.3 
                 200.0 
                 NaN 
                   
                   
               
               
                   
                  2nd 
                 −0.3 
                 200.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 200.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 2.3 
                 200.0 
                   
                 7.5 
                 0.0 
               
               
                   
                  2nd 
                 3.8 
                 200.0 
                   
                 7.4 
                 0.0 
               
               
                   
                 3rd 
                 2.3 
                 200.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 Mean 
                 2.8 
                 200.0 
                 #DIV/0! 
                 7.2 
                 0.0 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 431.3 
                 200.0 
                 15083.7 
                 6.1 
                 2472.7 
               
               
                   
                  2nd 
                 423.3 
                 200.0 
                 14810.7 
                 5.8 
                 2553.6 
               
               
                   
                 3rd 
                 407.3 
                 200.0 
                 14264.9 
                 5.5 
                 2593.6 
               
               
                   
                 Mean 
                 420.6 
                 200.0 
                 14719.8 
                 5.8 
                 2540.0 
               
               
                   
                 STDER 
                 7.1 
                 0.0 
                 240.7 
                 0.2 
                 35.5 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 4.3 
                 200.0 
                   
                 5.2 
                 0.0 
               
               
                   
                  2nd 
                 2.3 
                 200.0 
                   
                 4.8 
                 0.0 
               
               
                   
                 Mean 
                 3.3 
                 200.0 
                 #DIV/0! 
                 5.0 
                 0.0 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 #DIV/0! 
                 0.2 
                 0.0 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 1.3 
                 200.0 
                   
                 4.4 
                 0.0 
               
               
                   
                  2nd 
                 1.3 
                 200.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 Mean 
                 1.3 
                 200.0 
                 #DIV/0! 
                 5.1 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.7 
                 0.0 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 2.3 
                 200.0 
                   
                 5.5 
                 0.0 
               
               
                   
                  2nd 
                 1.3 
                 200.0 
                   
                 5.5 
                 0.0 
               
               
                   
                 Mean 
                 1.8 
                 200.0 
                 #DIV/0! 
                 5.5 
                 0.0 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 0.0 
                 0.0 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 27.3 
                 200.0 
                 880.7 
                 6.4 
                 137.6 
               
               
                   
                  2nd 
                 3.3 
                 200.0 
                   
                 6.1 
                 0.0 
               
               
                   
                 Mean 
                 15.3 
                 200.0 
                 880.7 
                 6.3 
                 68.8 
               
               
                   
                 STDER 
                 12.0 
                 0.0 
                 #DIV/0! 
                 0.2 
                 68.8 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 19.8 
                 200.0 
                 524.7 
                 6.4 
                 82.0 
               
               
                   
                  2nd 
                 11.8 
                 200.0 
                   
                 7.0 
                 0.0 
               
               
                   
                 Mean 
                 15.8 
                 200.0 
                 524.7 
                 6.7 
                 41.0 
               
               
                   
                 STDER 
                 4.0 
                 0.0 
                 #DIV/0! 
                 0.3 
                 41.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 415.8 
                 200.0 
                 14554.8 
                 6.2 
                 2347.5 
               
               
                 Xiidra 0.001 uM 
                  2nd 
                 365.3 
                 200.0 
                 12833.4 
                 7.6 
                 1688.6 
               
               
                   
                 Mean 
                 390.5 
                 200.0 
                 13694.1 
                 6.9 
                 2018.1 
               
               
                   
                 STDER 
                 25.3 
                 0.0 
                 860.7 
                 0.7 
                 329.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 427.3 
                 200.0 
                 14947.2 
                 5.2 
                 2874.5 
               
               
                 Xiidra 0.01 uM 
                  2nd 
                 380.3 
                 200.0 
                 13344.5 
                 5.8 
                 2300.8 
               
               
                   
                 Mean 
                 403.8 
                 200.0 
                 14145.8 
                 5.5 
                 2587.6 
               
               
                   
                 STDER 
                 23.5 
                 0.0 
                 801.4 
                 0.3 
                 286.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 367.8 
                 200.0 
                 12918.6 
                 6.6 
                 1957.4 
               
               
                 Xiidra 0.1 uM 
                  2nd 
                 384.3 
                 200.0 
                 13480.8 
                 5.7 
                 2365.0 
               
               
                   
                 Mean 
                 376.0 
                 200.0 
                 13199.7 
                 6.2 
                 2161.2 
               
               
                   
                 STDER 
                 8.3 
                 0.0 
                 281.1 
                 0.5 
                 203.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 405.8 
                 200.0 
                 14213.7 
                 4.8 
                 2961.2 
               
               
                 Xiidra 1 uM 
                  2nd 
                 428.8 
                 200.0 
                 14998.4 
                 4.4 
                 3408.7 
               
               
                   
                 Mean 
                 417.3 
                 200.0 
                 14606.0 
                 4.6 
                 3185.0 
               
               
                   
                 STDER 
                 11.5 
                 0.0 
                 392.3 
                 0.2 
                 223.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 431.3 
                 200.0 
                 15083.7 
                 5.8 
                 2600.6 
               
               
                 Xiidra 10 uM 
                  2nd 
                 400.3 
                 200.0 
                 14026.2 
                 6.4 
                 2191.6 
               
               
                   
                 Mean 
                 415.8 
                 200.0 
                 14554.9 
                 6.1 
                 2396.1 
               
               
                   
                 STDER 
                 15.5 
                 0.0 
                 528.8 
                 0.3 
                 204.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 407.8 
                 200.0 
                 14281.9 
                 7.1 
                 2011.5 
               
               
                 Xiidra 100 uM 
                  2nd 
                 426.3 
                 200.0 
                 14913.1 
                 5.8 
                 2571.2 
               
               
                   
                 Mean 
                 417.0 
                 200.0 
                 14597.5 
                 6.5 
                 2291.4 
               
               
                   
                 STDER 
                 9.3 
                 0.0 
                 315.6 
                 0.7 
                 279.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 371.3 
                 200.0 
                 13037.8 
                 5.7 
                 2287.3 
               
               
                 YY101 0.001 uM 
                  2nd 
                 359.8 
                 200.0 
                 12646.1 
                 7.1 
                 1781.1 
               
               
                   
                 3rd 
                 335.3 
                 200.0 
                 11811.7 
                 6.6 
                 1789.6 
               
               
                   
                 Mean 
                 355.4 
                 200.0 
                 12498.5 
                 6.5 
                 1952.7 
               
               
                   
                 STDER 
                 10.6 
                 0.0 
                 361.6 
                 0.4 
                 167.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 378.3 
                 200.0 
                 13276.3 
                 6.4 
                 2074.4 
               
               
                 YY101 0.01 uM 
                  2nd 
                 385.8 
                 200.0 
                 13531.9 
                 6.0 
                 2255.3 
               
               
                   
                 3rd 
                 373.3 
                 200.0 
                 13106.0 
                 6.1 
                 2148.5 
               
               
                   
                 Mean 
                 379.1 
                 200.0 
                 13304.7 
                 6.2 
                 2159.4 
               
               
                   
                 STDER 
                 3.6 
                 0.0 
                 123.8 
                 0.1 
                 52.5 
               
               
                   
                 1st 
                 353.8 
                 200.0 
                 12441.7 
                 6.2 
                 2006.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                  2nd 
                 374.8 
                 200.0 
                 13157.1 
                 5.2 
                 2530.2 
               
               
                 YY101 0.1 uM 
                 3rd 
                 365.8 
                 200.0 
                 12850.5 
                 6.2 
                 2072.7 
               
               
                   
                 Mean 
                 364.8 
                 200.0 
                 12816.4 
                 5.9 
                 2203.2 
               
               
                   
                 STDER 
                 6.1 
                 0.0 
                 207.2 
                 0.3 
                 164.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 377.3 
                 200.0 
                 13242.2 
                 6.3 
                 2101.9 
               
               
                 YY101 1 uM 
                  2nd 
                 366.3 
                 200.0 
                 12867.5 
                 7.1 
                 1812.3 
               
               
                   
                 3rd 
                 368.8 
                 200.0 
                 12952.6 
                 6.2 
                 2089.1 
               
               
                   
                 Mean 
                 370.8 
                 200.0 
                 13020.8 
                 6.5 
                 2001.1 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 113.4 
                 0.3 
                 94.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 398.8 
                 200.0 
                 13975.0 
                 6.0 
                 2329.2 
               
               
                 YY101 10 uM 
                  2nd 
                 430.3 
                 200.0 
                 15049.6 
                 6.4 
                 2351.5 
               
               
                   
                 3rd 
                 371.3 
                 200.0 
                 13037.8 
                 7.6 
                 1715.5 
               
               
                   
                 Mean 
                 400.1 
                 200.0 
                 14020.8 
                 6.7 
                 2132.1 
               
               
                   
                 STDER 
                 17.0 
                 0.0 
                 581.2 
                 0.5 
                 208.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 462.3 
                 200.0 
                 16142.7 
                 6.3 
                 2562.3 
               
               
                 YY101 100 uM 
                  2nd 
                 442.3 
                 200.0 
                 15459.3 
                 6.2 
                 2493.4 
               
               
                   
                 3rd 
                 428.8 
                 200.0 
                 14998.4 
                 5.0 
                 2999.7 
               
               
                   
                 Mean 
                 444.4 
                 200.0 
                 15533.5 
                 5.8 
                 2685.2 
               
               
                   
                 STDER 
                 9.7 
                 0.0 
                 332.4 
                 0.4 
                 158.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 179.8 
                 200.0 
                 6493.4 
                 4.5 
                 1443.0 
               
               
                 YDE011 0.001 uM 
                  2nd 
                 185.3 
                 200.0 
                 6683.4 
                 5.8 
                 1152.3 
               
               
                   
                 3rd 
                 194.8 
                 200.0 
                 7011.0 
                 5.8 
                 1208.8 
               
               
                   
                 Mean 
                 186.6 
                 200.0 
                 6729.3 
                 5.4 
                 1268.0 
               
               
                   
                 STDER 
                 4.4 
                 0.0 
                 151.1 
                 0.4 
                 89.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 201.3 
                 200.0 
                 7234.7 
                 6.3 
                 1148.4 
               
               
                 YDE011 0.01 uM 
                  2nd 
                 198.3 
                 200.0 
                 7131.5 
                 6.2 
                 1150.2 
               
               
                   
                 3rd 
                 192.3 
                 200.0 
                 6924.8 
                 5.1 
                 1357.8 
               
               
                   
                 Mean 
                 197.3 
                 200.0 
                 7097.0 
                 5.9 
                 1218.8 
               
               
                   
                 STDER 
                 2.6 
                 0.0 
                 91.1 
                 0.4 
                 69.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 178.8 
                 200.0 
                 6458.9 
                 6.6 
                 978.6 
               
               
                 YDE011 0.1 uM 
                  2nd 
                 191.8 
                 200.0 
                 6907.6 
                 5.0 
                 1381.5 
               
               
                   
                 3rd 
                 186.8 
                 200.0 
                 6735.2 
                 5.7 
                 1181.6 
               
               
                   
                 Mean 
                 185.8 
                 200.0 
                 6700.6 
                 5.8 
                 1180.6 
               
               
                   
                 STDER 
                 3.8 
                 0.0 
                 130.7 
                 0.5 
                 116.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 189.3 
                 200.0 
                 6821.4 
                 5.5 
                 1240.3 
               
               
                 YDE011 1 uM 
                  2nd 
                 200.8 
                 200.0 
                 7217.5 
                 4.8 
                 1503.6 
               
               
                   
                 3rd 
                 179.3 
                 200.0 
                 6476.2 
                 4.8 
                 1349.2 
               
               
                   
                 Mean 
                 189.8 
                 200.0 
                 6838.4 
                 5.0 
                 1364.4 
               
               
                   
                 STDER 
                 6.2 
                 0.0 
                 214.2 
                 0.2 
                 76.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 191.3 
                 200.0 
                 6890.4 
                 5.4 
                 1276.0 
               
               
                 YDE011 10 uM 
                  2nd 
                 185.8 
                 200.0 
                 6700.7 
                 5.8 
                 1155.3 
               
               
                   
                 3rd 
                 186.3 
                 200.0 
                 6717.9 
                 4.7 
                 1429.3 
               
               
                   
                 Mean 
                 187.8 
                 200.0 
                 6769.7 
                 5.3 
                 1286.9 
               
               
                   
                 STDER 
                 1.8 
                 0.0 
                 60.6 
                 0.3 
                 79.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 217.8 
                 200.0 
                 7801.4 
                 6.1 
                 1278.9 
               
               
                 YDE011 100 uM 
                  2nd 
                 215.3 
                 200.0 
                 7715.7 
                 6.1 
                 1264.9 
               
               
                   
                 3rd 
                 200.3 
                 200.0 
                 7200.3 
                 6.0 
                 1200.1 
               
               
                   
                 Mean 
                 211.1 
                 200.0 
                 7572.5 
                 6.1 
                 1247.9 
               
               
                   
                 STDER 
                 5.5 
                 0.0 
                 187.7 
                 0.0 
                 24.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 175.8 
                 200.0 
                 6355.1 
                 4.4 
                 1444.3 
               
               
                 YDE043 0.001 uM 
                  2nd 
                 211.3 
                 200.0 
                 7578.4 
                 6.0 
                 1263.1 
               
               
                   
                 3rd 
                 204.3 
                 200.0 
                 7337.9 
                 4.6 
                 1595.2 
               
               
                   
                 Mean 
                 197.1 
                 200.0 
                 7090.4 
                 5.0 
                 1434.2 
               
               
                   
                 STDER 
                 10.9 
                 0.0 
                 374.2 
                 0.5 
                 96.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 196.3 
                 200.0 
                 7062.6 
                 4.5 
                 1569.5 
               
               
                 YDE043 0.01 uM 
                  2nd 
                 194.8 
                 200.0 
                 7011.0 
                 6.6 
                 1062.3 
               
               
                   
                 3rd 
                 187.3 
                 200.0 
                 6752.4 
                 5.0 
                 1350.5 
               
               
                   
                 Mean 
                 192.8 
                 200.0 
                 6942.0 
                 5.4 
                 1327.4 
               
               
                   
                 STDER 
                 2.8 
                 0.0 
                 96.0 
                 0.6 
                 146.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 180.8 
                 200.0 
                 6528.0 
                 5.0 
                 1305.6 
               
               
                 YDE043 0.1 uM 
                  2nd 
                 169.8 
                 200.0 
                 6147.3 
                 5.6 
                 1097.7 
               
               
                   
                 3rd 
                 197.3 
                 200.0 
                 7097.1 
                 5.6 
                 1267.3 
               
               
                   
                 Mean 
                 182.6 
                 200.0 
                 6590.8 
                 5.4 
                 1223.6 
               
               
                   
                 STDER 
                 8.0 
                 0.0 
                 276.0 
                 0.2 
                 63.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 198.3 
                 200.0 
                 7131.5 
                 5.8 
                 1229.6 
               
               
                 YDE043 1 uM 
                  2nd 
                 170.3 
                 200.0 
                 6164.6 
                 4.8 
                 1284.3 
               
               
                   
                 3rd 
                 214.3 
                 200.0 
                 7681.3 
                 5.3 
                 1449.3 
               
               
                   
                 Mean 
                 194.3 
                 200.0 
                 6992.5 
                 5.3 
                 1321.1 
               
               
                   
                 STDER 
                 12.9 
                 0.0 
                 443.3 
                 0.3 
                 66.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 203.3 
                 200.0 
                 7303.5 
                 5.2 
                 1404.5 
               
               
                 YDE043 10 uM 
                  2nd 
                 415.8 
                 200.0 
                 14554.8 
                 5.4 
                 2695.3 
               
               
                   
                 3rd 
                 185.3 
                 200.0 
                 6683.4 
                 5.6 
                 1193.5 
               
               
                   
                 Mean 
                 268.1 
                 200.0 
                 9513.9 
                 5.4 
                 1764.4 
               
               
                   
                 STDER 
                 74.0 
                 0.0 
                 2526.8 
                 0.1 
                 469.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 205.8 
                 200.0 
                 7389.4 
                 6.2 
                 1191.8 
               
               
                 YDE043 100 uM 
                  2nd 
                 199.8 
                 200.0 
                 7183.1 
                 6.4 
                 1122.4 
               
               
                   
                 Mean 
                 202.8 
                 200.0 
                 7286.3 
                 6.3 
                 1157.1 
               
               
                   
                 STDER 
                 3.0 
                 0.0 
                 103.2 
                 0.1 
                 34.7 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 30 
               
             
            
               
                   
               
               
                 Bead name: TNF-alpha 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.0 
                 10.0 
                 NaN 
                   
                   
               
               
                   
                  2nd 
                 0.0 
                 10.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 10.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 1.0 
                 10.0 
                 16.4 
                 7.5 
                 2.2 
               
               
                   
                  2nd 
                 2.0 
                 10.0 
                 19.0 
                 7.4 
                 2.6 
               
               
                   
                 3rd 
                 1.5 
                 10.0 
                 17.7 
                 6.6 
                 2.7 
               
               
                   
                 Mean 
                 1.5 
                 10.0 
                 17.7 
                 7.2 
                 2.5 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.7 
                 0.3 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 1068.5 
                 10.0 
                 3196.1 
                 6.1 
                 524.0 
               
               
                   
                  2nd 
                 986.0 
                 10.0 
                 2941.3 
                 5.8 
                 507.1 
               
               
                   
                 3rd 
                 943.5 
                 10.0 
                 2810.4 
                 5.5 
                 511.0 
               
               
                   
                 Mean 
                 999.3 
                 10.0 
                 2982.6 
                 5.8 
                 514.0 
               
               
                   
                 STDER 
                 36.7 
                 0.0 
                 113.2 
                 0.2 
                 5.1 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 5.0 
                 10.0 
                 26.8 
                 5.2 
                 5.1 
               
               
                   
                  2nd 
                 6.0 
                 10.0 
                 29.4 
                 4.8 
                 6.1 
               
               
                   
                 Mean 
                 5.5 
                 10.0 
                 28.1 
                 5.0 
                 5.6 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 1.3 
                 0.2 
                 0.5 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 5.0 
                 10.0 
                 26.8 
                 4.4 
                 6.1 
               
               
                   
                  2nd 
                 7.0 
                 10.0 
                 32.0 
                 5.8 
                 5.5 
               
               
                   
                 Mean 
                 6.0 
                 10.0 
                 29.4 
                 5.1 
                 5.8 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 2.6 
                 0.7 
                 0.3 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 7.0 
                 10.0 
                 32.0 
                 5.5 
                 5.8 
               
               
                   
                  2nd 
                 8.0 
                 10.0 
                 34.6 
                 5.5 
                 6.3 
               
               
                   
                 Mean 
                 7.5 
                 10.0 
                 33.3 
                 5.5 
                 6.1 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 1.3 
                 0.0 
                 0.2 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 29.0 
                 10.0 
                 90.9 
                 6.4 
                 14.2 
               
               
                   
                  2nd 
                 7.0 
                 10.0 
                 32.0 
                 6.1 
                 5.2 
               
               
                   
                 Mean 
                 18.0 
                 10.0 
                 61.4 
                 6.3 
                 9.7 
               
               
                   
                 STDER 
                 11.0 
                 0.0 
                 29.4 
                 0.2 
                 4.5 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 30.5 
                 10.0 
                 95.0 
                 6.4 
                 14.8 
               
               
                   
                  2nd 
                 14.0 
                 10.0 
                 50.5 
                 7.0 
                 7.2 
               
               
                   
                 Mean 
                 22.3 
                 10.0 
                 72.7 
                 6.7 
                 11.0 
               
               
                   
                 STDER 
                 8.3 
                 0.0 
                 22.2 
                 0.3 
                 3.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 861.0 
                 10.0 
                 2557.1 
                 6.2 
                 412.4 
               
               
                 Xiidra 0.001 uM 
                  2nd 
                 841.0 
                 10.0 
                 2495.8 
                 7.6 
                 328.4 
               
               
                   
                 Mean 
                 851.0 
                 10.0 
                 2526.4 
                 6.9 
                 370.4 
               
               
                   
                 STDER 
                 10.0 
                 0.0 
                 30.6 
                 0.7 
                 42.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1000.0 
                 10.0 
                 2984.4 
                 5.2 
                 573.9 
               
               
                 Xiidra 0.01 uM 
                  2nd 
                 837.0 
                 10.0 
                 2483.6 
                 5.8 
                 428.2 
               
               
                   
                 Mean 
                 918.5 
                 10.0 
                 2734.0 
                 5.5 
                 501.1 
               
               
                   
                 STDER 
                 81.5 
                 0.0 
                 250.4 
                 0.3 
                 72.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 869.0 
                 10.0 
                 2581.6 
                 6.6 
                 391.1 
               
               
                 Xiidra 0.1 uM 
                  2nd 
                 751.0 
                 10.0 
                 2221.0 
                 5.7 
                 389.6 
               
               
                   
                 Mean 
                 810.0 
                 10.0 
                 2401.3 
                 6.2 
                 390.4 
               
               
                   
                 STDER 
                 59.0 
                 0.0 
                 180.3 
                 0.5 
                 0.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 942.5 
                 10.0 
                 2807.3 
                 4.8 
                 584.9 
               
               
                 Xiidra 1 uM 
                  2nd 
                 670.0 
                 10.0 
                 1974.8 
                 4.4 
                 448.8 
               
               
                   
                 Mean 
                 806.3 
                 10.0 
                 2391.0 
                 4.6 
                 516.8 
               
               
                   
                 STDER 
                 136.3 
                 0.0 
                 416.3 
                 0.2 
                 68.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1003.5 
                 10.0 
                 2995.2 
                 5.8 
                 516.4 
               
               
                 Xiidra 10 uM 
                  2nd 
                 964.0 
                 10.0 
                 2873.5 
                 6.4 
                 449.0 
               
               
                   
                 Mean 
                 983.8 
                 10.0 
                 2934.4 
                 6.1 
                 482.7 
               
               
                   
                 STDER 
                 19.8 
                 0.0 
                 60.9 
                 0.3 
                 33.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 802.0 
                 10.0 
                 2376.5 
                 7.1 
                 334.7 
               
               
                 Xiidra 100 uM 
                  2nd 
                 778.0 
                 10.0 
                 2303.3 
                 5.8 
                 397.1 
               
               
                   
                 Mean 
                 790.0 
                 10.0 
                 2339.9 
                 6.5 
                 365.9 
               
               
                   
                 STDER 
                 12.0 
                 0.0 
                 36.6 
                 0.7 
                 31.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 854.5 
                 10.0 
                 2537.1 
                 5.7 
                 445.1 
               
               
                 YY101 0.001 uM 
                  2nd 
                 878.0 
                 10.0 
                 2609.2 
                 7.1 
                 367.5 
               
               
                   
                 3rd 
                 729.0 
                 10.0 
                 2154.0 
                 6.6 
                 326.4 
               
               
                   
                 Mean 
                 820.5 
                 10.0 
                 2433.4 
                 6.5 
                 379.7 
               
               
                   
                 STDER 
                 46.3 
                 0.0 
                 141.3 
                 0.4 
                 34.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 946.5 
                 10.0 
                 2819.6 
                 6.4 
                 440.6 
               
               
                 YY101 0.01 uM 
                  2nd 
                 935.0 
                 10.0 
                 2784.2 
                 6.0 
                 464.0 
               
               
                   
                 3rd 
                 887.0 
                 10.0 
                 2636.8 
                 6.1 
                 432.3 
               
               
                   
                 Mean 
                 922.8 
                 10.0 
                 2746.9 
                 6.2 
                 445.6 
               
               
                   
                 STDER 
                 18.2 
                 0.0 
                 56.0 
                 0.1 
                 9.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 937.5 
                 10.0 
                 2791.9 
                 6.2 
                 450.3 
               
               
                 YY101 0.1 uM 
                  2nd 
                 963.0 
                 10.0 
                 2870.4 
                 5.2 
                 552.0 
               
               
                   
                 3rd 
                 971.0 
                 10.0 
                 2895.0 
                 6.2 
                 466.9 
               
               
                   
                 Mean 
                 957.2 
                 10.0 
                 2852.4 
                 5.9 
                 489.7 
               
               
                   
                 STDER 
                 10.1 
                 0.0 
                 31.1 
                 0.3 
                 31.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 920.0 
                 10.0 
                 2738.1 
                 6.3 
                 434.6 
               
               
                 YY101 1 uM 
                  2nd 
                 844.5 
                 10.0 
                 2506.5 
                 7.1 
                 353.0 
               
               
                   
                 3rd 
                 814.0 
                 10.0 
                 2413.2 
                 6.2 
                 389.2 
               
               
                   
                 Mean 
                 859.5 
                 10.0 
                 2552.6 
                 6.5 
                 392.3 
               
               
                   
                 STDER 
                 31.5 
                 0.0 
                 96.6 
                 0.3 
                 23.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 921.0 
                 10.0 
                 2741.2 
                 6.0 
                 456.9 
               
               
                 YY101 10 uM 
                  2nd 
                 942.5 
                 10.0 
                 2807.3 
                 6.4 
                 438.6 
               
               
                   
                 3rd 
                 905.0 
                 10.0 
                 2692.0 
                 7.6 
                 354.2 
               
               
                   
                 Mean 
                 922.8 
                 10.0 
                 2746.8 
                 6.7 
                 416.6 
               
               
                   
                 STDER 
                 10.9 
                 0.0 
                 33.4 
                 0.5 
                 31.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 865.0 
                 10.0 
                 2569.3 
                 6.3 
                 407.8 
               
               
                 YY101 100 uM 
                  2nd 
                 783.0 
                 10.0 
                 2318.5 
                 6.2 
                 374.0 
               
               
                   
                 3rd 
                 823.5 
                 10.0 
                 2442.3 
                 5.0 
                 488.5 
               
               
                   
                 Mean 
                 823.8 
                 10.0 
                 2443.4 
                 5.8 
                 423.4 
               
               
                   
                 STDER 
                 23.7 
                 0.0 
                 72.4 
                 0.4 
                 34.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 427.5 
                 10.0 
                 1245.3 
                 4.5 
                 276.7 
               
               
                 YDE011 0.001 uM 
                  2nd 
                 426.0 
                 10.0 
                 1240.9 
                 5.8 
                 213.9 
               
               
                   
                 3rd 
                 441.0 
                 10.0 
                 1285.6 
                 5.8 
                 221.7 
               
               
                   
                 Mean 
                 431.5 
                 10.0 
                 1257.3 
                 5.4 
                 237.4 
               
               
                   
                 STDER 
                 4.8 
                 0.0 
                 14.2 
                 0.4 
                 19.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 449.0 
                 10.0 
                 1309.5 
                 6.3 
                 207.9 
               
               
                 YDE011 0.01 uM 
                  2nd 
                 441.0 
                 10.0 
                 1285.6 
                 6.2 
                 207.4 
               
               
                   
                 3rd 
                 441.0 
                 10.0 
                 1285.6 
                 5.1 
                 252.1 
               
               
                   
                 Mean 
                 443.7 
                 10.0 
                 1293.5 
                 5.9 
                 222.4 
               
               
                   
                 STDER 
                 2.7 
                 0.0 
                 8.0 
                 0.4 
                 14.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 437.0 
                 10.0 
                 1273.7 
                 6.6 
                 193.0 
               
               
                 YDE011 0.1 uM 
                  2nd 
                 438.0 
                 10.0 
                 1276.6 
                 5.0 
                 255.3 
               
               
                   
                 3rd 
                 432.0 
                 10.0 
                 1258.7 
                 5.7 
                 220.8 
               
               
                   
                 Mean 
                 435.7 
                 10.0 
                 1269.7 
                 5.8 
                 223.0 
               
               
                   
                 STDER 
                 1.9 
                 0.0 
                 5.5 
                 0.5 
                 18.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 440.0 
                 10.0 
                 1282.6 
                 5.5 
                 233.2 
               
               
                 YDE011 1 uM 
                  2nd 
                 415.0 
                 10.0 
                 1208.1 
                 4.8 
                 251.7 
               
               
                   
                 3rd 
                 439.0 
                 10.0 
                 1279.6 
                 4.8 
                 266.6 
               
               
                   
                 Mean 
                 431.3 
                 10.0 
                 1256.8 
                 5.0 
                 250.5 
               
               
                   
                 STDER 
                 8.2 
                 0.0 
                 24.4 
                 0.2 
                 9.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 479.0 
                 10.0 
                 1399.2 
                 5.4 
                 259.1 
               
               
                 YDE011 10 uM 
                  2nd 
                 431.0 
                 10.0 
                 1255.8 
                 5.8 
                 216.5 
               
               
                   
                 3rd 
                 458.0 
                 10.0 
                 1336.4 
                 4.7 
                 284.3 
               
               
                   
                 Mean 
                 456.0 
                 10.0 
                 1330.4 
                 5.3 
                 253.3 
               
               
                   
                 STDER 
                 13.9 
                 0.0 
                 41.5 
                 0.3 
                 19.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 340.0 
                 10.0 
                 985.6 
                 6.1 
                 161.6 
               
               
                 YDE011 100 uM 
                  2nd 
                 331.5 
                 10.0 
                 960.5 
                 6.1 
                 157.5 
               
               
                   
                 3rd 
                 331.0 
                 10.0 
                 959.0 
                 6.0 
                 159.8 
               
               
                   
                 Mean 
                 334.2 
                 10.0 
                 968.4 
                 6.1 
                 159.6 
               
               
                   
                 STDER 
                 2.9 
                 0.0 
                 8.6 
                 0.0 
                 1.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 428.5 
                 10.0 
                 1248.3 
                 4.4 
                 283.7 
               
               
                 YDE043 0.001 uM 
                  2nd 
                 481.0 
                 10.0 
                 1405.1 
                 6.0 
                 234.2 
               
               
                   
                 3rd 
                 453.0 
                 10.0 
                 1321.4 
                 4.6 
                 287.3 
               
               
                   
                 Mean 
                 454.2 
                 10.0 
                 1325.0 
                 5.0 
                 268.4 
               
               
                   
                 STDER 
                 15.2 
                 0.0 
                 45.3 
                 0.5 
                 17.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 440.0 
                 10.0 
                 1282.6 
                 4.5 
                 285.0 
               
               
                 YDE043 0.01 uM 
                  2nd 
                 453.5 
                 10.0 
                 1322.9 
                 6.6 
                 200.4 
               
               
                   
                 3rd 
                 471.5 
                 10.0 
                 1376.7 
                 5.0 
                 275.3 
               
               
                   
                 Mean 
                 455.0 
                 10.0 
                 1327.4 
                 5.4 
                 253.6 
               
               
                   
                 STDER 
                 9.1 
                 0.0 
                 27.3 
                 0.6 
                 26.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 430.5 
                 10.0 
                 1254.3 
                 5.0 
                 250.9 
               
               
                 YDE043 0.1 uM 
                  2nd 
                 457.0 
                 10.0 
                 1333.4 
                 5.6 
                 238.1 
               
               
                   
                 3rd 
                 469.0 
                 10.0 
                 1369.2 
                 5.6 
                 244.5 
               
               
                   
                 Mean 
                 452.2 
                 10.0 
                 1319.0 
                 5.4 
                 244.5 
               
               
                   
                 STDER 
                 11.4 
                 0.0 
                 34.0 
                 0.2 
                 3.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 456.0 
                 10.0 
                 1330.4 
                 5.8 
                 229.4 
               
               
                 YDE043 1 uM 
                  2nd 
                 391.0 
                 10.0 
                 1136.7 
                 4.8 
                 236.8 
               
               
                   
                 3rd 
                 451.0 
                 10.0 
                 1315.4 
                 5.3 
                 248.2 
               
               
                   
                 Mean 
                 432.7 
                 10.0 
                 1260.8 
                 5.3 
                 238.1 
               
               
                   
                 STDER 
                 20.9 
                 0.0 
                 62.2 
                 0.3 
                 5.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 404.0 
                 10.0 
                 1175.4 
                 5.2 
                 226.0 
               
               
                 YDE043 10 uM 
                  2nd 
                 429.0 
                 10.0 
                 1249.8 
                 5.4 
                 231.4 
               
               
                   
                 3rd 
                 452.0 
                 10.0 
                 1318.4 
                 5.6 
                 235.4 
               
               
                   
                 Mean 
                 428.3 
                 10.0 
                 1247.9 
                 5.4 
                 231.0 
               
               
                   
                 STDER 
                 13.9 
                 0.0 
                 41.3 
                 0.1 
                 2.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 330.0 
                 10.0 
                 956.0 
                 6.2 
                 154.2 
               
               
                 YDE043 100 uM 
                  2nd 
                 293.0 
                 10.0 
                 847.0 
                 6.4 
                 132.4 
               
               
                   
                 Mean 
                 311.5 
                 10.0 
                 901.5 
                 6.3 
                 143.3 
               
               
                   
                 STDER 
                 18.5 
                 0.0 
                 54.5 
                 0.1 
                 10.9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 31 
               
             
            
               
                   
               
               
                 Bead name: CCL3/MIP-1 alpha 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.5 
                 10.0 
                 NaN 
                   
                   
               
               
                   
                  2nd 
                 −0.5 
                 10.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 10.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 1st 
                 −0.5 
                 10.0 
                   
                 7.5 
                 0.0 
               
               
                 PBMC + 0.5% DMSO 
                  2nd 
                 −0.5 
                 10.0 
                   
                 7.4 
                 0.0 
               
               
                   
                 3rd 
                 −0.5 
                 10.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 Mean 
                 −0.5 
                 10.0 
                 #DIV/0! 
                 7.2 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 2832.5 
                 10.0 
                 33012.9 
                 6.1 
                 5411.9 
               
               
                   
                  2nd 
                 2469.5 
                 10.0 
                 30553.0 
                 5.8 
                 5267.8 
               
               
                   
                 3rd 
                 2329.0 
                 10.0 
                 29577.8 
                 5.5 
                 5377.8 
               
               
                   
                 Mean 
                 2543.7 
                 10.0 
                 31047.9 
                 5.8 
                 5352.5 
               
               
                   
                 STDER 
                 150.0 
                 0.0 
                 1022.0 
                 0.2 
                 43.5 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 0.5 
                 10.0 
                   
                 5.2 
                 0.0 
               
               
                   
                  2nd 
                 −1.0 
                 10.0 
                   
                 4.8 
                 0.0 
               
               
                   
                 Mean 
                 −0.3 
                 10.0 
                 #DIV/0! 
                 5.0 
                 0.0 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 #DIV/0! 
                 0.2 
                 0.0 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 −0.5 
                 10.0 
                   
                 4.4 
                 0.0 
               
               
                   
                  2nd 
                 −0.5 
                 10.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 Mean 
                 −0.5 
                 10.0 
                 #DIV/0! 
                 5.1 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.7 
                 0.0 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 1.5 
                 10.0 
                 588.0 
                 5.5 
                 106.9 
               
               
                   
                  2nd 
                 0.5 
                 10.0 
                   
                 5.5 
                 0.0 
               
               
                   
                 Mean 
                 1.0 
                 10.0 
                 588.0 
                 5.5 
                 53.5 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 0.0 
                 53.5 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 7.5 
                 10.0 
                 1997.0 
                 6.4 
                 312.0 
               
               
                   
                  2nd 
                 −0.5 
                 10.0 
                   
                 6.1 
                 0.0 
               
               
                   
                 Mean 
                 3.5 
                 10.0 
                 1997.0 
                 6.3 
                 156.0 
               
               
                   
                 STDER 
                 4.0 
                 0.0 
                 #DIV/0! 
                 0.2 
                 156.0 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 2.5 
                 10.0 
                 1030.2 
                 6.4 
                 161.0 
               
               
                   
                  2nd 
                 −0.5 
                 10.0 
                   
                 7.0 
                 0.0 
               
               
                   
                 Mean 
                 1.0 
                 10.0 
                 1030.2 
                 6.7 
                 80.5 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 #DIV/0! 
                 0.3 
                 80.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2391.5 
                 10.0 
                 30013.4 
                 6.2 
                 4840.9 
               
               
                 Xiidra 0.001 uM 
                  2nd 
                 2296.5 
                 10.0 
                 29350.1 
                 7.6 
                 3861.9 
               
               
                   
                 Mean 
                 2344.0 
                 10.0 
                 29681.8 
                 6.9 
                 4351.4 
               
               
                   
                 STDER 
                 47.5 
                 0.0 
                 331.7 
                 0.7 
                 489.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2786.0 
                 10.0 
                 32701.9 
                 5.2 
                 6288.8 
               
               
                 Xiidra 0.01 uM 
                  2nd 
                 2444.5 
                 10.0 
                 30380.5 
                 5.8 
                 5238.0 
               
               
                   
                 Mean 
                 2615.3 
                 10.0 
                 31541.2 
                 5.5 
                 5763.4 
               
               
                   
                 STDER 
                 170.8 
                 0.0 
                 1160.7 
                 0.3 
                 525.4 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2010.0 
                 10.0 
                 27301.7 
                 6.6 
                 4136.6 
               
               
                 Xiidra 0.1 uM 
                  2nd 
                 1672.5 
                 10.0 
                 24770.7 
                 5.7 
                 4345.7 
               
               
                   
                 Mean 
                 1841.3 
                 10.0 
                 26036.2 
                 6.2 
                 4241.2 
               
               
                   
                 STDER 
                 168.8 
                 0.0 
                 1265.5 
                 0.5 
                 104.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1962.5 
                 10.0 
                 26954.1 
                 4.8 
                 5615.4 
               
               
                 Xiidra 1 uM 
                  2nd 
                 1468.5 
                 10.0 
                 23157.9 
                 4.4 
                 5263.2 
               
               
                   
                 Mean 
                 1715.5 
                 10.0 
                 25056.0 
                 4.6 
                 5439.3 
               
               
                   
                 STDER 
                 247.0 
                 0.0 
                 1898.1 
                 0.2 
                 176.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1875.5 
                 10.0 
                 26310.6 
                 5.8 
                 4536.3 
               
               
                 Xiidra 10 uM 
                  2nd 
                 1893.5 
                 10.0 
                 26444.5 
                 6.4 
                 4131.9 
               
               
                   
                 Mean 
                 1884.5 
                 10.0 
                 26377.5 
                 6.1 
                 4334.1 
               
               
                   
                 STDER 
                 9.0 
                 0.0 
                 66.9 
                 0.3 
                 202.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 1943.0 
                 10.0 
                 26810.6 
                 7.1 
                 3776.1 
               
               
                 Xiidra 100 uM 
                  2nd 
                 1557.5 
                 10.0 
                 23870.5 
                 5.8 
                 4115.6 
               
               
                   
                 Mean 
                 1750.3 
                 10.0 
                 25340.6 
                 6.5 
                 3945.9 
               
               
                   
                 STDER 
                 192.8 
                 0.0 
                 1470.0 
                 0.7 
                 169.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2104.5 
                 10.0 
                 27986.1 
                 5.7 
                 4909.8 
               
               
                 YY101 0.001 uM 
                  2nd 
                 1995.5 
                 10.0 
                 27195.8 
                 7.1 
                 3830.4 
               
               
                   
                 3rd 
                 1764.5 
                 10.0 
                 25475.7 
                 6.6 
                 3860.0 
               
               
                   
                 Mean 
                 1954.8 
                 10.0 
                 26885.9 
                 6.5 
                 4200.1 
               
               
                   
                 STDER 
                 100.2 
                 0.0 
                 741.1 
                 0.4 
                 355.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2293.5 
                 10.0 
                 29329.0 
                 6.4 
                 4582.7 
               
               
                 YY101 0.01 uM 
                  2nd 
                 2303.0 
                 10.0 
                 29395.7 
                 6.0 
                 4899.3 
               
               
                   
                 3rd 
                 2116.5 
                 10.0 
                 28072.3 
                 6.1 
                 4602.0 
               
               
                   
                 Mean 
                 2237.7 
                 10.0 
                 28932.4 
                 6.2 
                 4694.7 
               
               
                   
                 STDER 
                 60.6 
                 0.0 
                 430.4 
                 0.1 
                 102.5 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2157.0 
                 10.0 
                 28362.4 
                 6.2 
                 4574.6 
               
               
                 YY101 0.1 uM 
                  2nd 
                 2199.5 
                 10.0 
                 28665.1 
                 5.2 
                 5512.5 
               
               
                   
                 3rd 
                 2154.5 
                 10.0 
                 28344.5 
                 6.2 
                 4571.7 
               
               
                   
                 Mean 
                 2170.3 
                 10.0 
                 28457.4 
                 5.9 
                 4886.3 
               
               
                   
                 STDER 
                 14.6 
                 0.0 
                 104.0 
                 0.3 
                 313.1 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2206.5 
                 10.0 
                 28714.8 
                 6.3 
                 4557.9 
               
               
                 YY101 1 uM 
                  2nd 
                 2029.0 
                 10.0 
                 27440.0 
                 7.1 
                 3864.8 
               
               
                   
                 3rd 
                 2109.5 
                 10.0 
                 28022.0 
                 6.2 
                 4519.7 
               
               
                   
                 Mean 
                 2115.0 
                 10.0 
                 28059.0 
                 6.5 
                 4314.1 
               
               
                   
                 STDER 
                 51.3 
                 0.0 
                 368.5 
                 0.3 
                 224.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 2406.5 
                 10.0 
                 30117.5 
                 6.0 
                 5019.6 
               
               
                 YY101 10 uM 
                  2nd 
                 2501.0 
                 10.0 
                 30769.7 
                 6.4 
                 4807.8 
               
               
                   
                 3rd 
                 2251.0 
                 10.0 
                 29029.8 
                 7.6 
                 3819.7 
               
               
                   
                 Mean 
                 2386.2 
                 10.0 
                 29972.3 
                 6.7 
                 4549.0 
               
               
                   
                 STDER 
                 72.9 
                 0.0 
                 507.5 
                 0.5 
                 369.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 3190.5 
                 10.0 
                 35374.6 
                 6.3 
                 5615.0 
               
               
                 YY101 100 uM 
                  2nd 
                 2648.5 
                 10.0 
                 31775.7 
                 6.2 
                 5125.1 
               
               
                   
                 3rd 
                 2798.0 
                 10.0 
                 32782.2 
                 5.0 
                 6556.4 
               
               
                   
                 Mean 
                 2879.0 
                 10.0 
                 33310.8 
                 5.8 
                 5765.5 
               
               
                   
                 STDER 
                 161.6 
                 0.0 
                 1072.0 
                 0.4 
                 420.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 134.5 
                 10.0 
                 7525.9 
                 4.5 
                 1672.4 
               
               
                 YDE011 0.001 uM 
                  2nd 
                 126.5 
                 10.0 
                 7321.9 
                 5.8 
                 1262.4 
               
               
                   
                 3rd 
                 137.0 
                 10.0 
                 7588.4 
                 5.8 
                 1308.3 
               
               
                   
                 Mean 
                 132.7 
                 10.0 
                 7478.7 
                 5.4 
                 1414.4 
               
               
                   
                 STDER 
                 3.2 
                 0.0 
                 80.5 
                 0.4 
                 129.7 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 138.5 
                 10.0 
                 7625.5 
                 6.3 
                 1210.4 
               
               
                 YDE011 0.01 uM 
                  2nd 
                 137.5 
                 10.0 
                 7600.8 
                 6.2 
                 1225.9 
               
               
                   
                 3rd 
                 134.5 
                 10.0 
                 7525.9 
                 5.1 
                 1475.7 
               
               
                   
                 Mean 
                 136.8 
                 10.0 
                 7584.1 
                 5.9 
                 1304.0 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 29.9 
                 0.4 
                 86.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 113.5 
                 10.0 
                 6974.8 
                 6.6 
                 1056.8 
               
               
                 YDE011 0.1 uM 
                  2nd 
                 127.0 
                 10.0 
                 7334.8 
                 5.0 
                 1467.0 
               
               
                   
                 3rd 
                 114.5 
                 10.0 
                 7002.2 
                 5.7 
                 1228.5 
               
               
                   
                 Mean 
                 118.3 
                 10.0 
                 7103.9 
                 5.8 
                 1250.7 
               
               
                   
                 STDER 
                 4.3 
                 0.0 
                 115.7 
                 0.5 
                 118.9 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 129.5 
                 10.0 
                 7399.2 
                 5.5 
                 1345.3 
               
               
                 YDE011 1 uM 
                  2nd 
                 138.0 
                 10.0 
                 7613.2 
                 4.8 
                 1586.1 
               
               
                   
                 3rd 
                 137.5 
                 10.0 
                 7600.8 
                 4.8 
                 1583.5 
               
               
                   
                 Mean 
                 135.0 
                 10.0 
                 7537.7 
                 5.0 
                 1505.0 
               
               
                   
                 STDER 
                 2.8 
                 0.0 
                 69.4 
                 0.2 
                 79.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 152.5 
                 10.0 
                 7962.5 
                 5.4 
                 1474.5 
               
               
                 YDE011 10 uM 
                  2nd 
                 128.5 
                 10.0 
                 7373.5 
                 5.8 
                 1271.3 
               
               
                   
                 3rd 
                 148.5 
                 10.0 
                 7868.0 
                 4.7 
                 1674.0 
               
               
                   
                 Mean 
                 143.2 
                 10.0 
                 7734.7 
                 5.3 
                 1473.3 
               
               
                   
                 STDER 
                 7.4 
                 0.0 
                 182.6 
                 0.3 
                 116.3 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 164.5 
                 10.0 
                 8238.4 
                 6.1 
                 1350.6 
               
               
                 YDE011 100 uM 
                  2nd 
                 157.0 
                 10.0 
                 8067.3 
                 6.1 
                 1322.5 
               
               
                   
                 3rd 
                 137.5 
                 10.0 
                 7600.8 
                 6.0 
                 1266.8 
               
               
                   
                 Mean 
                 153.0 
                 10.0 
                 7968.8 
                 6.1 
                 1313.3 
               
               
                   
                 STDER 
                 8.0 
                 0.0 
                 190.5 
                 0.0 
                 24.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 139.0 
                 10.0 
                 7637.9 
                 4.4 
                 1735.9 
               
               
                 YDE043 0.001 uM 
                  2nd 
                 163.0 
                 10.0 
                 8204.6 
                 6.0 
                 1367.4 
               
               
                   
                 3rd 
                 152.5 
                 10.0 
                 7962.5 
                 4.6 
                 1731.0 
               
               
                   
                 Mean 
                 151.5 
                 10.0 
                 7935.0 
                 5.0 
                 1611.4 
               
               
                   
                 STDER 
                 6.9 
                 0.0 
                 164.2 
                 0.5 
                 122.0 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 145.5 
                 10.0 
                 7796.2 
                 4.5 
                 1732.5 
               
               
                 YDE043 0.01 uM 
                  2nd 
                 153.5 
                 10.0 
                 7985.9 
                 6.6 
                 1210.0 
               
               
                   
                 3rd 
                 148.5 
                 10.0 
                 7868.0 
                 5.0 
                 1573.6 
               
               
                   
                 Mean 
                 149.2 
                 10.0 
                 7883.4 
                 5.4 
                 1505.4 
               
               
                   
                 STDER 
                 2.3 
                 0.0 
                 55.3 
                 0.6 
                 154.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 134.0 
                 10.0 
                 7513.4 
                 5.0 
                 1502.7 
               
               
                 YDE043 0.1 uM 
                  2nd 
                 138.5 
                 10.0 
                 7625.5 
                 5.6 
                 1361.7 
               
               
                   
                 3rd 
                 145.5 
                 10.0 
                 7796.2 
                 5.6 
                 1392.2 
               
               
                   
                 Mean 
                 139.3 
                 10.0 
                 7645.0 
                 5.4 
                 1418.9 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 82.2 
                 0.2 
                 42.8 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 152.5 
                 10.0 
                 7962.5 
                 5.8 
                 1372.8 
               
               
                 YDE043 1 uM 
                  2nd 
                 110.5 
                 10.0 
                 6891.7 
                 4.8 
                 1435.8 
               
               
                   
                 3rd 
                 156.5 
                 10.0 
                 8055.7 
                 5.3 
                 1520.0 
               
               
                   
                 Mean 
                 139.8 
                 10.0 
                 7636.6 
                 5.3 
                 1442.9 
               
               
                   
                 STDER 
                 14.7 
                 0.0 
                 373.5 
                 0.3 
                 42.6 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 133.5 
                 10.0 
                 7500.8 
                 5.2 
                 1442.5 
               
               
                 YDE043 10 uM 
                  2nd 
                 131.5 
                 10.0 
                 7450.2 
                 5.4 
                 1379.7 
               
               
                   
                 3rd 
                 148.5 
                 10.0 
                 7868.0 
                 5.6 
                 1405.0 
               
               
                   
                 Mean 
                 137.8 
                 10.0 
                 7606.3 
                 5.4 
                 1409.0 
               
               
                   
                 STDER 
                 5.4 
                 0.0 
                 131.6 
                 0.1 
                 18.2 
               
               
                 PBMC + LPS(5 ug/ml) + 
                 1st 
                 152.5 
                 10.0 
                 7962.5 
                 6.2 
                 1284.3 
               
               
                 YDE043 100 uM 
                  2nd 
                 136.5 
                 10.0 
                 7575.9 
                 6.4 
                 1183.7 
               
               
                   
                 Mean 
                 144.5 
                 10.0 
                 7769.2 
                 6.3 
                 1234.0 
               
               
                   
                 STDER 
                 8.0 
                 0.0 
                 193.3 
                 0.1 
                 50.3 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 32 
               
             
            
               
                   
               
               
                 Bead name: CCL4/MIP-1 beta 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.0 
                 10.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.0 
                 10.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 10.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 0.0 
                 10.0 
                   
                 7.5 
                 0.0 
               
               
                   
                 2nd 
                 0.0 
                 10.0 
                   
                 7.4 
                 0.0 
               
               
                   
                 3rd 
                 0.0 
                 10.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 Mean 
                 0.0 
                 10.0 
                 #DIV/0! 
                 7.2 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 794.0 
                 10.0 
                 18382.1 
                 6.1 
                 3013.5 
               
               
                   
                 2nd 
                 724.0 
                 10.0 
                 17299.3 
                 5.8 
                 2982.6 
               
               
                   
                 3rd 
                 697.5 
                 10.0 
                 16886.0 
                 5.5 
                 3070.2 
               
               
                   
                 Mean 
                 738.5 
                 10.0 
                 17522.4 
                 5.8 
                 3022.1 
               
               
                   
                 STDER 
                 28.8 
                 0.0 
                 446.1 
                 0.2 
                 25.6 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 0.0 
                 10.0 
                   
                 5.2 
                 0.0 
               
               
                   
                 2nd 
                 0.0 
                 10.0 
                   
                 4.8 
                 0.0 
               
               
                   
                 Mean 
                 0.0 
                 10.0 
                 #DIV/0! 
                 5.0 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.2 
                 0.0 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 0.0 
                 10.0 
                   
                 4.4 
                 0.0 
               
               
                   
                 2nd 
                 0.0 
                 10.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 Mean 
                 0.0 
                 10.0 
                 #DIV/0! 
                 5.1 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.7 
                 0.0 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 −1.0 
                 10.0 
                   
                 5.5 
                 0.0 
               
               
                   
                 2nd 
                 −0.5 
                 10.0 
                   
                 5.5 
                 0.0 
               
               
                   
                 Mean 
                 −0.8 
                 10.0 
                 #DIV/0! 
                 5.5 
                 0.0 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 #DIV/0! 
                 0.0 
                 0.0 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 10.0 
                 10.0 
                 1948.5 
                 6.4 
                 304.4 
               
               
                   
                 2nd 
                 1.0 
                 10.0 
                   
                 6.1 
                 0.0 
               
               
                   
                 Mean 
                 5.5 
                 10.0 
                 1948.5 
                 6.3 
                 152.2 
               
               
                   
                 STDER 
                 4.5 
                 0.0 
                 #DIV/0! 
                 0.2 
                 152.2 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 10.0 
                 10.0 
                 1948.5 
                 6.4 
                 304.4 
               
               
                   
                 2nd 
                 3.0 
                 10.0 
                 772.9 
                 7.0 
                 110.4 
               
               
                   
                 Mean 
                 6.5 
                 10.0 
                 1360.7 
                 6.7 
                 207.4 
               
               
                   
                 STDER 
                 3.5 
                 0.0 
                 587.8 
                 0.3 
                 97.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 615.0 
                 10.0 
                 15584.4 
                 6.2 
                 2513.6 
               
               
                   
                 2nd 
                 573.0 
                 10.0 
                 14911.0 
                 7.6 
                 1962.0 
               
               
                   
                 Mean 
                 594.0 
                 10.0 
                 15247.7 
                 6.9 
                 2237.8 
               
               
                   
                 STDER 
                 21.0 
                 0.0 
                 336.7 
                 0.7 
                 275.8 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 700.0 
                 10.0 
                 16925.1 
                 5.2 
                 3254.8 
               
               
                   
                 2nd 
                 628.0 
                 10.0 
                 15791.2 
                 5.8 
                 2722.6 
               
               
                   
                 Mean 
                 664.0 
                 10.0 
                 16358.1 
                 5.5 
                 2988.7 
               
               
                   
                 STDER 
                 36.0 
                 0.0 
                 566.9 
                 0.3 
                 266.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 524.0 
                 10.0 
                 14113.8 
                 6.6 
                 2138.5 
               
               
                   
                 2nd 
                 442.0 
                 10.0 
                 12744.4 
                 5.7 
                 2235.9 
               
               
                   
                 Mean 
                 483.0 
                 10.0 
                 13429.1 
                 6.2 
                 2187.2 
               
               
                   
                 STDER 
                 41.0 
                 0.0 
                 684.7 
                 0.5 
                 48.7 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 599.0 
                 10.0 
                 15328.8 
                 4.8 
                 3193.5 
               
               
                   
                 2nd 
                 397.0 
                 10.0 
                 11968.5 
                 4.4 
                 2720.1 
               
               
                   
                 Mean 
                 498.0 
                 10.0 
                 13648.6 
                 4.6 
                 2956.8 
               
               
                   
                 STDER 
                 101.0 
                 0.0 
                 1680.2 
                 0.2 
                 236.7 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 630.0 
                 10.0 
                 15822.9 
                 5.8 
                 2728.1 
               
               
                   
                 2nd 
                 629.0 
                 10.0 
                 15807.1 
                 6.4 
                 2469.9 
               
               
                   
                 Mean 
                 629.5 
                 10.0 
                 15815.0 
                 6.1 
                 2599.0 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 7.9 
                 0.3 
                 129.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 631.5 
                 10.0 
                 15846.7 
                 7.1 
                 2231.9 
               
               
                   
                 2nd 
                 565.5 
                 10.0 
                 14789.8 
                 5.8 
                 2550.0 
               
               
                   
                 Mean 
                 598.5 
                 10.0 
                 15318.3 
                 6.5 
                 2391.0 
               
               
                   
                 STDER 
                 33.0 
                 0.0 
                 528.5 
                 0.7 
                 159.0 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 551.5 
                 10.0 
                 14562.9 
                 5.7 
                 2554.9 
               
               
                   
                 2nd 
                 527.0 
                 10.0 
                 14163.0 
                 7.1 
                 1994.8 
               
               
                   
                 3rd 
                 469.0 
                 10.0 
                 13200.9 
                 6.6 
                 2000.1 
               
               
                   
                 Mean 
                 515.8 
                 10.0 
                 13975.6 
                 6.5 
                 2183.3 
               
               
                   
                 STDER 
                 24.5 
                 0.0 
                 404.2 
                 0.4 
                 185.8 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 611.0 
                 10.0 
                 15520.6 
                 6.4 
                 2425.1 
               
               
                   
                 2nd 
                 596.5 
                 10.0 
                 15288.8 
                 6.0 
                 2548.1 
               
               
                   
                 3rd 
                 560.0 
                 10.0 
                 14700.8 
                 6.1 
                 2410.0 
               
               
                   
                 Mean 
                 589.2 
                 10.0 
                 15170.0 
                 6.2 
                 2461.1 
               
               
                   
                 STDER 
                 15.2 
                 0.0 
                 244.0 
                 0.1 
                 43.8 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 576.0 
                 10.0 
                 14959.4 
                 6.2 
                 2412.8 
               
               
                   
                 2nd 
                 592.0 
                 10.0 
                 15216.6 
                 5.2 
                 2926.3 
               
               
                   
                 3rd 
                 584.0 
                 10.0 
                 15088.1 
                 6.2 
                 2433.6 
               
               
                   
                 Mean 
                 584.0 
                 10.0 
                 15088.0 
                 5.9 
                 2590.9 
               
               
                   
                 STDER 
                 4.6 
                 0.0 
                 74.3 
                 0.3 
                 167.8 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 594.0 
                 10.0 
                 15248.7 
                 6.3 
                 2420.4 
               
               
                   
                 2nd 
                 556.5 
                 10.0 
                 14644.0 
                 7.1 
                 2062.5 
               
               
                   
                 3rd 
                 547.0 
                 10.0 
                 14489.7 
                 6.2 
                 2337.0 
               
               
                   
                 Mean 
                 565.8 
                 10.0 
                 14794.1 
                 6.5 
                 2273.3 
               
               
                   
                 STDER 
                 14.3 
                 0.0 
                 231.6 
                 0.3 
                 108.1 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 614.0 
                 10.0 
                 15568.4 
                 6.0 
                 2594.7 
               
               
                   
                 2nd 
                 651.0 
                 10.0 
                 16155.4 
                 6.4 
                 2524.3 
               
               
                   
                 3rd 
                 560.0 
                 10.0 
                 14700.8 
                 7.6 
                 1934.3 
               
               
                   
                 Mean 
                 608.3 
                 10.0 
                 15474.9 
                 6.7 
                 2351.1 
               
               
                   
                 STDER 
                 26.4 
                 0.0 
                 422.5 
                 0.5 
                 209.4 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 537.0 
                 10.0 
                 14326.7 
                 6.3 
                 2274.1 
               
               
                   
                 2nd 
                 474.5 
                 10.0 
                 13293.2 
                 6.2 
                 2144.1 
               
               
                   
                 3rd 
                 488.0 
                 10.0 
                 13518.7 
                 5.0 
                 2703.7 
               
               
                   
                 Mean 
                 499.8 
                 10.0 
                 13712.9 
                 5.8 
                 2374.0 
               
               
                   
                 STDER 
                 19.0 
                 0.0 
                 313.7 
                 0.4 
                 169.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 158.0 
                 10.0 
                 7267.3 
                 4.5 
                 1615.0 
               
               
                   
                 2nd 
                 156.0 
                 10.0 
                 7220.3 
                 5.8 
                 1244.9 
               
               
                   
                 3rd 
                 158.0 
                 10.0 
                 7267.3 
                 5.8 
                 1253.0 
               
               
                   
                 Mean 
                 157.3 
                 10.0 
                 7251.7 
                 5.4 
                 1370.9 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 15.7 
                 0.4 
                 122.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 167.5 
                 10.0 
                 7487.6 
                 6.3 
                 1188.5 
               
               
                   
                 2nd 
                 164.0 
                 10.0 
                 7407.0 
                 6.2 
                 1194.7 
               
               
                   
                 3rd 
                 159.0 
                 10.0 
                 7290.8 
                 5.1 
                 1429.6 
               
               
                   
                 Mean 
                 163.5 
                 10.0 
                 7395.1 
                 5.9 
                 1270.9 
               
               
                   
                 STDER 
                 2.5 
                 0.0 
                 57.1 
                 0.4 
                 79.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 152.0 
                 10.0 
                 7125.7 
                 6.6 
                 1079.6 
               
               
                   
                 2nd 
                 151.0 
                 10.0 
                 7101.9 
                 5.0 
                 1420.4 
               
               
                   
                 3rd 
                 141.0 
                 10.0 
                 6860.2 
                 5.7 
                 1203.6 
               
               
                   
                 Mean 
                 148.0 
                 10.0 
                 7029.3 
                 5.8 
                 1234.5 
               
               
                   
                 STDER 
                 3.5 
                 0.0 
                 84.8 
                 0.5 
                 99.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 151.0 
                 10.0 
                 7101.9 
                 5.5 
                 1291.2 
               
               
                   
                 2nd 
                 152.0 
                 10.0 
                 7125.7 
                 4.8 
                 1484.5 
               
               
                   
                 3rd 
                 158.0 
                 10.0 
                 7267.3 
                 4.8 
                 1514.0 
               
               
                   
                 Mean 
                 153.7 
                 10.0 
                 7165.0 
                 5.0 
                 1429.9 
               
               
                   
                 STDER 
                 2.2 
                 0.0 
                 51.7 
                 0.2 
                 69.9 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 166.0 
                 10.0 
                 7453.1 
                 5.4 
                 1380.2 
               
               
                   
                 2nd 
                 153.5 
                 10.0 
                 7161.3 
                 5.8 
                 1234.7 
               
               
                   
                 3rd 
                 161.5 
                 10.0 
                 7349.0 
                 4.7 
                 1563.6 
               
               
                   
                 Mean 
                 160.3 
                 10.0 
                 7321.1 
                 5.3 
                 1392.8 
               
               
                   
                 STDER 
                 3.7 
                 0.0 
                 85.4 
                 0.3 
                 95.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 119.0 
                 10.0 
                 6303.5 
                 6.1 
                 1033.4 
               
               
                   
                 2nd 
                 121.5 
                 10.0 
                 6368.7 
                 6.1 
                 1044.1 
               
               
                   
                 3rd 
                 115.0 
                 10.0 
                 6197.9 
                 6.0 
                 1033.0 
               
               
                   
                 Mean 
                 118.5 
                 10.0 
                 6290.1 
                 6.1 
                 1036.8 
               
               
                   
                 STDER 
                 1.9 
                 0.0 
                 49.8 
                 0.0 
                 3.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 165.5 
                 10.0 
                 7441.6 
                 4.4 
                 1691.3 
               
               
                   
                 2nd 
                 185.0 
                 10.0 
                 7881.5 
                 6.0 
                 1313.6 
               
               
                   
                 3rd 
                 182.5 
                 10.0 
                 7826.1 
                 4.6 
                 1701.3 
               
               
                   
                 Mean 
                 177.7 
                 10.0 
                 7716.4 
                 5.0 
                 1568.7 
               
               
                   
                 STDER 
                 6.1 
                 0.0 
                 138.3 
                 0.5 
                 127.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 178.0 
                 10.0 
                 7725.6 
                 4.5 
                 1716.8 
               
               
                   
                 2nd 
                 177.0 
                 10.0 
                 7703.2 
                 6.6 
                 1167.1 
               
               
                   
                 3rd 
                 183.0 
                 10.0 
                 7837.2 
                 5.0 
                 1567.4 
               
               
                   
                 Mean 
                 179.3 
                 10.0 
                 7755.3 
                 5.4 
                 1483.8 
               
               
                   
                 STDER 
                 1.9 
                 0.0 
                 41.4 
                 0.6 
                 164.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 153.5 
                 10.0 
                 7161.3 
                 5.0 
                 1432.3 
               
               
                   
                 2nd 
                 159.5 
                 10.0 
                 7302.4 
                 5.6 
                 1304.0 
               
               
                   
                 3rd 
                 173.0 
                 10.0 
                 7612.9 
                 5.6 
                 1359.5 
               
               
                   
                 Mean 
                 162.0 
                 10.0 
                 7358.9 
                 5.4 
                 1365.2 
               
               
                   
                 STDER 
                 5.8 
                 0.0 
                 133.4 
                 0.2 
                 37.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 175.0 
                 10.0 
                 7658.2 
                 5.8 
                 1320.4 
               
               
                   
                 2nd 
                 137.0 
                 10.0 
                 6761.7 
                 4.8 
                 1408.7 
               
               
                   
                 3rd 
                 175.0 
                 10.0 
                 7658.2 
                 5.3 
                 1444.9 
               
               
                   
                 Mean 
                 162.3 
                 10.0 
                 7359.3 
                 5.3 
                 1391.3 
               
               
                   
                 STDER 
                 12.7 
                 0.0 
                 298.8 
                 0.3 
                 37.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 159.0 
                 10.0 
                 7290.8 
                 5.2 
                 1402.1 
               
               
                   
                 2nd 
                 158.0 
                 10.0 
                 7267.3 
                 5.4 
                 1345.8 
               
               
                   
                 3rd 
                 161.0 
                 10.0 
                 7337.4 
                 5.6 
                 1310.3 
               
               
                   
                 Mean 
                 159.3 
                 10.0 
                 7298.5 
                 5.4 
                 1352.7 
               
               
                   
                 STDER 
                 0.9 
                 0.0 
                 20.6 
                 0.1 
                 26.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 118.5 
                 10.0 
                 6290.4 
                 6.2 
                 1014.6 
               
               
                   
                 2nd 
                 104.0 
                 10.0 
                 5899.5 
                 6.4 
                 921.8 
               
               
                   
                 Mean 
                 111.3 
                 10.0 
                 6094.9 
                 6.3 
                 968.2 
               
               
                   
                 STDER 
                 7.3 
                 0.0 
                 195.4 
                 0.1 
                 46.4 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 33 
               
             
            
               
                   
               
               
                 Bead name: CCL5/RANTES 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.5 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 −0.5 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 49.5 
                 2.0 
                 35.7 
                 7.5 
                 4.8 
               
               
                   
                 2nd 
                 42.5 
                 2.0 
                 31.0 
                 7.4 
                 4.2 
               
               
                   
                 3rd 
                 31.0 
                 2.0 
                 22.5 
                 6.6 
                 3.4 
               
               
                   
                 Mean 
                 41.0 
                 2.0 
                 29.7 
                 7.2 
                 4.1 
               
               
                   
                 STDER 
                 5.4 
                 0.0 
                 3.9 
                 0.3 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 1262.5 
                 2.0 
                 448.6 
                 6.1 
                 73.5 
               
               
                   
                 2nd 
                 1084.5 
                 2.0 
                 396.8 
                 5.8 
                 68.4 
               
               
                   
                 3rd 
                 1296.0 
                 2.0 
                 458.2 
                 5.5 
                 83.3 
               
               
                   
                 Mean 
                 1214.3 
                 2.0 
                 434.5 
                 5.8 
                 75.1 
               
               
                   
                 STDER 
                 65.6 
                 0.0 
                 19.1 
                 0.2 
                 4.4 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 37.5 
                 2.0 
                 27.5 
                 5.2 
                 5.3 
               
               
                   
                 2nd 
                 66.5 
                 2.0 
                 46.1 
                 4.8 
                 9.6 
               
               
                   
                 Mean 
                 52.0 
                 2.0 
                 36.8 
                 5.0 
                 7.4 
               
               
                   
                 STDER 
                 14.5 
                 0.0 
                 9.3 
                 0.2 
                 2.2 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 43.5 
                 2.0 
                 31.7 
                 4.4 
                 7.2 
               
               
                   
                 2nd 
                 57.5 
                 2.0 
                 40.7 
                 5.8 
                 7.0 
               
               
                   
                 Mean 
                 50.5 
                 2.0 
                 36.2 
                 5.1 
                 7.1 
               
               
                   
                 STDER 
                 7.0 
                 0.0 
                 4.5 
                 0.7 
                 0.1 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 45.5 
                 2.0 
                 33.0 
                 5.5 
                 6.0 
               
               
                   
                 2nd 
                 52.5 
                 2.0 
                 37.6 
                 5.5 
                 6.8 
               
               
                   
                 Mean 
                 49.0 
                 2.0 
                 35.3 
                 5.5 
                 6.4 
               
               
                   
                 STDER 
                 3.5 
                 0.0 
                 2.3 
                 0.0 
                 0.4 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 201.5 
                 2.0 
                 109.6 
                 6.4 
                 17.1 
               
               
                   
                 2nd 
                 70.5 
                 2.0 
                 48.3 
                 6.1 
                 7.9 
               
               
                   
                 Mean 
                 136.0 
                 2.0 
                 79.0 
                 6.3 
                 12.5 
               
               
                   
                 STDER 
                 65.5 
                 0.0 
                 30.6 
                 0.2 
                 4.6 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 131.5 
                 2.0 
                 79.2 
                 6.4 
                 12.4 
               
               
                   
                 2nd 
                 82.5 
                 2.0 
                 55.0 
                 7.0 
                 7.9 
               
               
                   
                 Mean 
                 107.0 
                 2.0 
                 67.1 
                 6.7 
                 10.1 
               
               
                   
                 STDER 
                 24.5 
                 0.0 
                 12.1 
                 0.3 
                 2.3 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 1222.5 
                 2.0 
                 437.0 
                 6.2 
                 70.5 
               
               
                   
                 2nd 
                 1145.5 
                 2.0 
                 414.6 
                 7.6 
                 54.6 
               
               
                   
                 Mean 
                 1184.0 
                 2.0 
                 425.8 
                 6.9 
                 62.5 
               
               
                   
                 STDER 
                 38.5 
                 0.0 
                 11.2 
                 0.7 
                 8.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 1212.5 
                 2.0 
                 434.1 
                 5.2 
                 83.5 
               
               
                   
                 2nd 
                 1054.0 
                 2.0 
                 387.9 
                 5.8 
                 66.9 
               
               
                   
                 Mean 
                 1133.3 
                 2.0 
                 411.0 
                 5.5 
                 75.2 
               
               
                   
                 STDER 
                 79.3 
                 0.0 
                 23.1 
                 0.3 
                 8.3 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 904.5 
                 2.0 
                 343.6 
                 6.6 
                 52.1 
               
               
                   
                 2nd 
                 898.0 
                 2.0 
                 341.6 
                 5.7 
                 59.9 
               
               
                   
                 Mean 
                 901.3 
                 2.0 
                 342.6 
                 6.2 
                 56.0 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 1.0 
                 0.5 
                 3.9 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 1248.5 
                 2.0 
                 444.5 
                 4.8 
                 92.6 
               
               
                   
                 2nd 
                 763.5 
                 2.0 
                 300.9 
                 4.4 
                 68.4 
               
               
                   
                 Mean 
                 1006.0 
                 2.0 
                 372.7 
                 4.6 
                 80.5 
               
               
                   
                 STDER 
                 242.5 
                 0.0 
                 71.8 
                 0.2 
                 12.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 959.5 
                 2.0 
                 360.0 
                 5.8 
                 62.1 
               
               
                   
                 2nd 
                 1078.5 
                 2.0 
                 395.1 
                 6.4 
                 61.7 
               
               
                   
                 Mean 
                 1019.0 
                 2.0 
                 377.5 
                 6.1 
                 61.9 
               
               
                   
                 STDER 
                 59.5 
                 0.0 
                 17.5 
                 0.3 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 903.5 
                 2.0 
                 343.3 
                 7.1 
                 48.4 
               
               
                   
                 2nd 
                 947.5 
                 2.0 
                 356.4 
                 5.8 
                 61.5 
               
               
                   
                 Mean 
                 925.5 
                 2.0 
                 349.9 
                 6.5 
                 54.9 
               
               
                   
                 STDER 
                 22.0 
                 0.0 
                 6.6 
                 0.7 
                 6.6 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 1120.5 
                 2.0 
                 407.4 
                 5.7 
                 71.5 
               
               
                   
                 2nd 
                 1139.5 
                 2.0 
                 412.9 
                 7.1 
                 58.2 
               
               
                   
                 3rd 
                 1049.5 
                 2.0 
                 386.6 
                 6.6 
                 58.6 
               
               
                   
                 Mean 
                 1103.2 
                 2.0 
                 402.3 
                 6.5 
                 62.7 
               
               
                   
                 STDER 
                 27.4 
                 0.0 
                 8.0 
                 0.4 
                 4.4 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 1358.5 
                 2.0 
                 476.2 
                 6.4 
                 74.4 
               
               
                   
                 2nd 
                 1246.5 
                 2.0 
                 443.9 
                 6.0 
                 74.0 
               
               
                   
                 3rd 
                 1240.5 
                 2.0 
                 442.2 
                 6.1 
                 72.5 
               
               
                   
                 Mean 
                 1281.8 
                 2.0 
                 454.1 
                 6.2 
                 73.6 
               
               
                   
                 STDER 
                 38.4 
                 0.0 
                 11.0 
                 0.1 
                 0.6 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 1206.5 
                 2.0 
                 432.4 
                 6.2 
                 69.7 
               
               
                   
                 2nd 
                 1188.5 
                 2.0 
                 427.1 
                 5.2 
                 82.1 
               
               
                   
                 3rd 
                 1179.5 
                 2.0 
                 424.5 
                 6.2 
                 68.5 
               
               
                   
                 Mean 
                 1191.5 
                 2.0 
                 428.0 
                 5.9 
                 73.5 
               
               
                   
                 STDER 
                 7.9 
                 0.0 
                 2.3 
                 0.3 
                 4.4 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 1289.0 
                 2.0 
                 456.2 
                 6.3 
                 72.4 
               
               
                   
                 2nd 
                 1161.5 
                 2.0 
                 419.3 
                 7.1 
                 59.1 
               
               
                   
                 3rd 
                 1151.5 
                 2.0 
                 416.4 
                 6.2 
                 67.2 
               
               
                   
                 Mean 
                 1200.7 
                 2.0 
                 430.6 
                 6.5 
                 66.2 
               
               
                   
                 STDER 
                 44.3 
                 0.0 
                 12.8 
                 0.3 
                 3.9 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 1173.5 
                 2.0 
                 422.8 
                 6.0 
                 70.5 
               
               
                   
                 2nd 
                 1264.5 
                 2.0 
                 449.1 
                 6.4 
                 70.2 
               
               
                   
                 3rd 
                 1158.5 
                 2.0 
                 418.4 
                 7.6 
                 55.1 
               
               
                   
                 Mean 
                 1198.8 
                 2.0 
                 430.1 
                 6.7 
                 65.2 
               
               
                   
                 STDER 
                 33.1 
                 0.0 
                 9.6 
                 0.5 
                 5.1 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 875.0 
                 2.0 
                 334.7 
                 6.3 
                 53.1 
               
               
                   
                 2nd 
                 860.5 
                 2.0 
                 330.4 
                 6.2 
                 53.3 
               
               
                   
                 3rd 
                 909.5 
                 2.0 
                 345.1 
                 5.0 
                 69.0 
               
               
                   
                 Mean 
                 881.7 
                 2.0 
                 336.7 
                 5.8 
                 58.5 
               
               
                   
                 STDER 
                 14.5 
                 0.0 
                 4.4 
                 0.4 
                 5.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 455.5 
                 2.0 
                 202.5 
                 4.5 
                 45.0 
               
               
                   
                 2nd 
                 455.5 
                 2.0 
                 202.5 
                 5.8 
                 34.9 
               
               
                   
                 3rd 
                 520.5 
                 2.0 
                 224.1 
                 5.8 
                 38.6 
               
               
                   
                 Mean 
                 477.2 
                 2.0 
                 209.7 
                 5.4 
                 39.5 
               
               
                   
                 STDER 
                 21.7 
                 0.0 
                 7.2 
                 0.4 
                 2.9 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 476.0 
                 2.0 
                 209.4 
                 6.3 
                 33.2 
               
               
                   
                 2nd 
                 497.5 
                 2.0 
                 216.5 
                 6.2 
                 34.9 
               
               
                   
                 3rd 
                 508.5 
                 2.0 
                 220.2 
                 5.1 
                 43.2 
               
               
                   
                 Mean 
                 494.0 
                 2.0 
                 215.3 
                 5.9 
                 37.1 
               
               
                   
                 STDER 
                 9.5 
                 0.0 
                 3.2 
                 0.4 
                 3.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 468.0 
                 2.0 
                 206.7 
                 6.6 
                 31.3 
               
               
                   
                 2nd 
                 463.5 
                 2.0 
                 205.2 
                 5.0 
                 41.0 
               
               
                   
                 3rd 
                 475.0 
                 2.0 
                 209.0 
                 5.7 
                 36.7 
               
               
                   
                 Mean 
                 468.8 
                 2.0 
                 207.0 
                 5.8 
                 36.3 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 1.1 
                 0.5 
                 2.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 464.5 
                 2.0 
                 205.5 
                 5.5 
                 37.4 
               
               
                   
                 2nd 
                 446.5 
                 2.0 
                 199.5 
                 4.8 
                 41.6 
               
               
                   
                 3rd 
                 431.5 
                 2.0 
                 194.4 
                 4.8 
                 40.5 
               
               
                   
                 Mean 
                 447.5 
                 2.0 
                 199.8 
                 5.0 
                 39.8 
               
               
                   
                 STDER 
                 9.5 
                 0.0 
                 3.2 
                 0.2 
                 1.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 492.0 
                 2.0 
                 214.7 
                 5.4 
                 39.8 
               
               
                   
                 2nd 
                 453.5 
                 2.0 
                 201.8 
                 5.8 
                 34.8 
               
               
                   
                 3rd 
                 442.5 
                 2.0 
                 198.1 
                 4.7 
                 42.1 
               
               
                   
                 Mean 
                 462.7 
                 2.0 
                 204.9 
                 5.3 
                 38.9 
               
               
                   
                 STDER 
                 15.0 
                 0.0 
                 5.0 
                 0.3 
                 2.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 362.5 
                 2.0 
                 170.4 
                 6.1 
                 27.9 
               
               
                   
                 2nd 
                 315.5 
                 2.0 
                 153.5 
                 6.1 
                 25.2 
               
               
                   
                 3rd 
                 337.5 
                 2.0 
                 161.5 
                 6.0 
                 26.9 
               
               
                   
                 Mean 
                 338.5 
                 2.0 
                 161.8 
                 6.1 
                 26.7 
               
               
                   
                 STDER 
                 13.6 
                 0.0 
                 4.9 
                 0.0 
                 0.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 445.5 
                 2.0 
                 199.1 
                 4.4 
                 45.3 
               
               
                   
                 2nd 
                 497.5 
                 2.0 
                 216.5 
                 6.0 
                 36.1 
               
               
                   
                 3rd 
                 465.0 
                 2.0 
                 205.7 
                 4.6 
                 44.7 
               
               
                   
                 Mean 
                 469.3 
                 2.0 
                 207.1 
                 5.0 
                 42.0 
               
               
                   
                 STDER 
                 15.2 
                 0.0 
                 5.1 
                 0.5 
                 3.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 506.0 
                 2.0 
                 219.3 
                 4.5 
                 48.7 
               
               
                   
                 2nd 
                 486.5 
                 2.0 
                 212.9 
                 6.6 
                 32.3 
               
               
                   
                 3rd 
                 483.0 
                 2.0 
                 211.7 
                 5.0 
                 42.3 
               
               
                   
                 Mean 
                 491.8 
                 2.0 
                 214.6 
                 5.4 
                 41.1 
               
               
                   
                 STDER 
                 7.2 
                 0.0 
                 2.4 
                 0.6 
                 4.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 508.5 
                 2.0 
                 220.2 
                 5.0 
                 44.0 
               
               
                   
                 2nd 
                 448.5 
                 2.0 
                 200.1 
                 5.6 
                 35.7 
               
               
                   
                 3rd 
                 530.5 
                 2.0 
                 227.4 
                 5.6 
                 40.6 
               
               
                   
                 Mean 
                 495.8 
                 2.0 
                 215.9 
                 5.4 
                 40.1 
               
               
                   
                 STDER 
                 24.5 
                 0.0 
                 8.1 
                 0.2 
                 2.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 502.5 
                 2.0 
                 218.2 
                 5.8 
                 37.6 
               
               
                   
                 2nd 
                 486.5 
                 2.0 
                 212.9 
                 4.8 
                 44.3 
               
               
                   
                 3rd 
                 484.5 
                 2.0 
                 212.2 
                 5.3 
                 40.0 
               
               
                   
                 Mean 
                 491.2 
                 2.0 
                 214.4 
                 5.3 
                 40.7 
               
               
                   
                 STDER 
                 5.7 
                 0.0 
                 1.9 
                 0.3 
                 2.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 486.5 
                 2.0 
                 212.9 
                 5.2 
                 40.9 
               
               
                   
                 2nd 
                 429.5 
                 2.0 
                 193.7 
                 5.4 
                 35.9 
               
               
                   
                 3rd 
                 453.5 
                 2.0 
                 201.8 
                 5.6 
                 36.0 
               
               
                   
                 Mean 
                 456.5 
                 2.0 
                 202.8 
                 5.4 
                 37.6 
               
               
                   
                 STDER 
                 16.5 
                 0.0 
                 5.6 
                 0.1 
                 1.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 353.0 
                 2.0 
                 167.0 
                 6.2 
                 26.9 
               
               
                   
                 2nd 
                 330.5 
                 2.0 
                 159.0 
                 6.4 
                 24.8 
               
               
                   
                 Mean 
                 341.8 
                 2.0 
                 163.0 
                 6.3 
                 25.9 
               
               
                   
                 STDER 
                 11.3 
                 0.0 
                 4.0 
                 0.1 
                 1.1 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 34 
               
             
            
               
                   
               
               
                 Bead name: IFN-gamma 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.0 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 1.0 
                 2.0 
                   
                 7.5 
                 0.0 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                   
                 7.4 
                 0.0 
               
               
                   
                 3rd 
                 0.0 
                 2.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 Mean 
                 0.7 
                 2.0 
                 #DIV/0! 
                 7.2 
                 0.0 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 473.0 
                 2.0 
                 1833.4 
                 6.1 
                 300.6 
               
               
                   
                 2nd 
                 476.0 
                 2.0 
                 1844.0 
                 5.8 
                 317.9 
               
               
                   
                 3rd 
                 552.5 
                 2.0 
                 2113.7 
                 5.5 
                 384.3 
               
               
                   
                 Mean 
                 500.5 
                 2.0 
                 1930.4 
                 5.8 
                 334.3 
               
               
                   
                 STDER 
                 26.0 
                 0.0 
                 91.7 
                 0.2 
                 25.5 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 5.0 
                 2.0 
                 12.5 
                 5.2 
                 2.4 
               
               
                   
                 2nd 
                 8.0 
                 2.0 
                 29.1 
                 4.8 
                 6.1 
               
               
                   
                 Mean 
                 6.5 
                 2.0 
                 20.8 
                 5.0 
                 4.2 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 8.3 
                 0.2 
                 1.8 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 5.0 
                 2.0 
                 12.5 
                 4.4 
                 2.8 
               
               
                   
                 2nd 
                 8.0 
                 2.0 
                 29.1 
                 5.8 
                 5.0 
               
               
                   
                 Mean 
                 6.5 
                 2.0 
                 20.8 
                 5.1 
                 3.9 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 8.3 
                 0.7 
                 1.1 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 5.0 
                 2.0 
                 12.5 
                 5.5 
                 2.3 
               
               
                   
                 2nd 
                 7.0 
                 2.0 
                 23.7 
                 5.5 
                 4.3 
               
               
                   
                 Mean 
                 6.0 
                 2.0 
                 18.1 
                 5.5 
                 3.3 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 5.6 
                 0.0 
                 1.0 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 15.5 
                 2.0 
                 67.3 
                 6.4 
                 10.5 
               
               
                   
                 2nd 
                 9.0 
                 2.0 
                 34.4 
                 6.1 
                 5.6 
               
               
                   
                 Mean 
                 12.3 
                 2.0 
                 50.9 
                 6.3 
                 8.1 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 16.5 
                 0.2 
                 2.4 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 12.0 
                 2.0 
                 49.9 
                 6.4 
                 7.8 
               
               
                   
                 2nd 
                 7.0 
                 2.0 
                 23.7 
                 7.0 
                 3.4 
               
               
                   
                 Mean 
                 9.5 
                 2.0 
                 36.8 
                 6.7 
                 5.6 
               
               
                   
                 STDER 
                 2.5 
                 0.0 
                 13.1 
                 0.3 
                 2.2 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 635.0 
                 2.0 
                 2400.7 
                 6.2 
                 387.2 
               
               
                   
                 2nd 
                 634.5 
                 2.0 
                 2399.0 
                 7.6 
                 315.7 
               
               
                   
                 Mean 
                 634.8 
                 2.0 
                 2399.8 
                 6.9 
                 351.4 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.9 
                 0.7 
                 35.8 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 690.0 
                 2.0 
                 2590.1 
                 5.2 
                 498.1 
               
               
                   
                 2nd 
                 498.5 
                 2.0 
                 1923.7 
                 5.8 
                 331.7 
               
               
                   
                 Mean 
                 594.3 
                 2.0 
                 2256.9 
                 5.5 
                 414.9 
               
               
                   
                 STDER 
                 95.8 
                 0.0 
                 333.2 
                 0.3 
                 83.2 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 360.0 
                 2.0 
                 1427.4 
                 6.6 
                 216.3 
               
               
                   
                 2nd 
                 428.0 
                 2.0 
                 1672.9 
                 5.7 
                 293.5 
               
               
                   
                 Mean 
                 394.0 
                 2.0 
                 1550.1 
                 6.2 
                 254.9 
               
               
                   
                 STDER 
                 34.0 
                 0.0 
                 122.8 
                 0.5 
                 38.6 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 344.0 
                 2.0 
                 1369.0 
                 4.8 
                 285.2 
               
               
                   
                 2nd 
                 285.0 
                 2.0 
                 1151.6 
                 4.4 
                 261.7 
               
               
                   
                 Mean 
                 314.5 
                 2.0 
                 1260.3 
                 4.6 
                 273.5 
               
               
                   
                 STDER 
                 29.5 
                 0.0 
                 108.7 
                 0.2 
                 11.7 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 208.0 
                 2.0 
                 861.4 
                 5.8 
                 148.5 
               
               
                   
                 2nd 
                 269.0 
                 2.0 
                 1092.0 
                 6.4 
                 170.6 
               
               
                   
                 Mean 
                 238.5 
                 2.0 
                 976.7 
                 6.1 
                 159.6 
               
               
                   
                 STDER 
                 30.5 
                 0.0 
                 115.3 
                 0.3 
                 11.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 123.0 
                 2.0 
                 529.0 
                 7.1 
                 74.5 
               
               
                   
                 2nd 
                 146.0 
                 2.0 
                 620.6 
                 5.8 
                 107.0 
               
               
                   
                 Mean 
                 134.5 
                 2.0 
                 574.8 
                 6.5 
                 90.8 
               
               
                   
                 STDER 
                 11.5 
                 0.0 
                 45.8 
                 0.7 
                 16.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 619.0 
                 2.0 
                 2345.3 
                 5.7 
                 411.5 
               
               
                   
                 2nd 
                 724.0 
                 2.0 
                 2706.6 
                 7.1 
                 381.2 
               
               
                   
                 3rd 
                 596.0 
                 2.0 
                 2265.5 
                 6.6 
                 343.3 
               
               
                   
                 Mean 
                 646.3 
                 2.0 
                 2439.1 
                 6.5 
                 378.6 
               
               
                   
                 STDER 
                 39.4 
                 0.0 
                 135.7 
                 0.4 
                 19.7 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 940.0 
                 2.0 
                 3435.7 
                 6.4 
                 536.8 
               
               
                   
                 2nd 
                 906.0 
                 2.0 
                 3322.0 
                 6.0 
                 553.7 
               
               
                   
                 3rd 
                 705.0 
                 2.0 
                 2641.6 
                 6.1 
                 433.0 
               
               
                   
                 Mean 
                 850.3 
                 2.0 
                 3133.1 
                 6.2 
                 507.8 
               
               
                   
                 STDER 
                 73.3 
                 0.0 
                 247.9 
                 0.1 
                 37.7 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 527.0 
                 2.0 
                 2024.2 
                 6.2 
                 326.5 
               
               
                   
                 2nd 
                 408.0 
                 2.0 
                 1601.1 
                 5.2 
                 307.9 
               
               
                   
                 3rd 
                 491.5 
                 2.0 
                 1899.0 
                 6.2 
                 306.3 
               
               
                   
                 Mean 
                 475.5 
                 2.0 
                 1841.4 
                 5.9 
                 313.6 
               
               
                   
                 STDER 
                 35.3 
                 0.0 
                 125.5 
                 0.3 
                 6.5 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 658.0 
                 2.0 
                 2480.1 
                 6.3 
                 393.7 
               
               
                   
                 2nd 
                 645.5 
                 2.0 
                 2437.0 
                 7.1 
                 343.2 
               
               
                   
                 3rd 
                 531.0 
                 2.0 
                 2038.3 
                 6.2 
                 328.8 
               
               
                   
                 Mean 
                 611.5 
                 2.0 
                 2318.4 
                 6.5 
                 355.2 
               
               
                   
                 STDER 
                 40.4 
                 0.0 
                 140.6 
                 0.3 
                 19.7 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 827.5 
                 2.0 
                 3058.1 
                 6.0 
                 509.7 
               
               
                   
                 2nd 
                 691.0 
                 2.0 
                 2593.6 
                 6.4 
                 405.2 
               
               
                   
                 3rd 
                 609.5 
                 2.0 
                 2312.4 
                 7.6 
                 304.3 
               
               
                   
                 Mean 
                 709.3 
                 2.0 
                 2654.7 
                 6.7 
                 406.4 
               
               
                   
                 STDER 
                 63.6 
                 0.0 
                 217.4 
                 0.5 
                 59.3 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 504.0 
                 2.0 
                 1943.2 
                 6.3 
                 308.4 
               
               
                   
                 2nd 
                 555.0 
                 2.0 
                 2122.4 
                 6.2 
                 342.3 
               
               
                   
                 3rd 
                 450.0 
                 2.0 
                 1751.5 
                 5.0 
                 350.3 
               
               
                   
                 Mean 
                 503.0 
                 2.0 
                 1939.0 
                 5.8 
                 333.7 
               
               
                   
                 STDER 
                 30.3 
                 0.0 
                 107.1 
                 0.4 
                 12.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 147.0 
                 2.0 
                 624.6 
                 4.5 
                 138.8 
               
               
                   
                 2nd 
                 174.0 
                 2.0 
                 730.3 
                 5.8 
                 125.9 
               
               
                   
                 3rd 
                 235.0 
                 2.0 
                 964.1 
                 5.8 
                 166.2 
               
               
                   
                 Mean 
                 185.3 
                 2.0 
                 773.0 
                 5.4 
                 143.6 
               
               
                   
                 STDER 
                 26.0 
                 0.0 
                 100.3 
                 0.4 
                 11.9 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 205.0 
                 2.0 
                 849.9 
                 6.3 
                 134.9 
               
               
                   
                 2nd 
                 185.0 
                 2.0 
                 773.0 
                 6.2 
                 124.7 
               
               
                   
                 3rd 
                 180.0 
                 2.0 
                 753.6 
                 5.1 
                 147.8 
               
               
                   
                 Mean 
                 190.0 
                 2.0 
                 792.2 
                 5.9 
                 135.8 
               
               
                   
                 STDER 
                 7.6 
                 0.0 
                 29.4 
                 0.4 
                 6.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 216.0 
                 2.0 
                 892.0 
                 6.6 
                 135.1 
               
               
                   
                 2nd 
                 180.0 
                 2.0 
                 753.6 
                 5.0 
                 150.7 
               
               
                   
                 3rd 
                 190.0 
                 2.0 
                 792.3 
                 5.7 
                 139.0 
               
               
                   
                 Mean 
                 195.3 
                 2.0 
                 812.6 
                 5.8 
                 141.6 
               
               
                   
                 STDER 
                 10.7 
                 0.0 
                 41.2 
                 0.5 
                 4.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 268.0 
                 2.0 
                 1088.2 
                 5.5 
                 197.9 
               
               
                   
                 2nd 
                 220.5 
                 2.0 
                 909.1 
                 4.8 
                 189.4 
               
               
                   
                 3rd 
                 235.0 
                 2.0 
                 964.1 
                 4.8 
                 200.9 
               
               
                   
                 Mean 
                 241.2 
                 2.0 
                 987.2 
                 5.0 
                 196.0 
               
               
                   
                 STDER 
                 14.1 
                 0.0 
                 53.0 
                 0.2 
                 3.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 237.5 
                 2.0 
                 973.6 
                 5.4 
                 180.3 
               
               
                   
                 2nd 
                 208.0 
                 2.0 
                 861.4 
                 5.8 
                 148.5 
               
               
                   
                 3rd 
                 163.5 
                 2.0 
                 689.4 
                 4.7 
                 146.7 
               
               
                   
                 Mean 
                 203.0 
                 2.0 
                 841.5 
                 5.3 
                 158.5 
               
               
                   
                 STDER 
                 21.5 
                 0.0 
                 82.6 
                 0.3 
                 10.9 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 142.0 
                 2.0 
                 604.8 
                 6.1 
                 99.1 
               
               
                   
                 2nd 
                 114.0 
                 2.0 
                 492.8 
                 6.1 
                 80.8 
               
               
                   
                 3rd 
                 117.0 
                 2.0 
                 504.9 
                 6.0 
                 84.1 
               
               
                   
                 Mean 
                 124.3 
                 2.0 
                 534.1 
                 6.1 
                 88.0 
               
               
                   
                 STDER 
                 8.9 
                 0.0 
                 35.5 
                 0.0 
                 5.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 208.5 
                 2.0 
                 863.3 
                 4.4 
                 196.2 
               
               
                   
                 2nd 
                 230.0 
                 2.0 
                 945.2 
                 6.0 
                 157.5 
               
               
                   
                 3rd 
                 185.5 
                 2.0 
                 774.9 
                 4.6 
                 168.5 
               
               
                   
                 Mean 
                 208.0 
                 2.0 
                 861.2 
                 5.0 
                 174.1 
               
               
                   
                 STDER 
                 12.8 
                 0.0 
                 49.2 
                 0.5 
                 11.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 207.0 
                 2.0 
                 857.6 
                 4.5 
                 190.6 
               
               
                   
                 2nd 
                 190.0 
                 2.0 
                 792.3 
                 6.6 
                 120.0 
               
               
                   
                 3rd 
                 211.5 
                 2.0 
                 874.8 
                 5.0 
                 175.0 
               
               
                   
                 Mean 
                 202.8 
                 2.0 
                 841.6 
                 5.4 
                 161.9 
               
               
                   
                 STDER 
                 6.5 
                 0.0 
                 25.1 
                 0.6 
                 21.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 216.0 
                 2.0 
                 892.0 
                 5.0 
                 178.4 
               
               
                   
                 2nd 
                 148.0 
                 2.0 
                 628.5 
                 5.6 
                 112.2 
               
               
                   
                 3rd 
                 211.0 
                 2.0 
                 872.9 
                 5.6 
                 155.9 
               
               
                   
                 Mean 
                 191.7 
                 2.0 
                 797.8 
                 5.4 
                 148.8 
               
               
                   
                 STDER 
                 21.9 
                 0.0 
                 84.8 
                 0.2 
                 19.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 175.0 
                 2.0 
                 734.2 
                 5.8 
                 126.6 
               
               
                   
                 2nd 
                 201.0 
                 2.0 
                 834.6 
                 4.8 
                 173.9 
               
               
                   
                 3rd 
                 241.5 
                 2.0 
                 988.7 
                 5.3 
                 186.5 
               
               
                   
                 Mean 
                 205.8 
                 2.0 
                 852.5 
                 5.3 
                 162.3 
               
               
                   
                 STDER 
                 19.3 
                 0.0 
                 74.0 
                 0.3 
                 18.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 199.5 
                 2.0 
                 828.9 
                 5.2 
                 159.4 
               
               
                   
                 2nd 
                 199.0 
                 2.0 
                 826.9 
                 5.4 
                 153.1 
               
               
                   
                 3rd 
                 209.0 
                 2.0 
                 865.3 
                 5.6 
                 154.5 
               
               
                   
                 Mean 
                 202.5 
                 2.0 
                 840.3 
                 5.4 
                 155.7 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 12.5 
                 0.1 
                 1.9 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 111.0 
                 2.0 
                 480.6 
                 6.2 
                 77.5 
               
               
                   
                 2nd 
                 102.0 
                 2.0 
                 444.0 
                 6.4 
                 69.4 
               
               
                   
                 Mean 
                 106.5 
                 2.0 
                 462.3 
                 6.3 
                 73.4 
               
               
                   
                 STDER 
                 4.5 
                 0.0 
                 18.3 
                 0.1 
                 4.1 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 35 
               
             
            
               
                   
               
               
                 Bead name: CCL2/JE/MCP-1 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Con- 
                 Live  
                 conc/  
               
               
                   
                   
                   
                   
                 cen- 
                 cell 
                 live 
               
               
                   
                   
                   
                 Dilu- 
                 tration 
                 (10{circumflex over ( )}5/ 
                 cell (pg/ 
               
               
                 Sample 
                   
                 MFI 
                 tion 
                 (pg/ml) 
                 ml) 
                 10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.0 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC +  
                 1st 
                 56.0 
                 2.0 
                 109.1 
                 7.5 
                 14.5 
               
               
                 0.5% DMSO 
                 2nd 
                 176.0 
                 2.0 
                 202.1 
                 7.4 
                 27.3 
               
               
                   
                 3rd 
                 181.0 
                 2.0 
                 205.3 
                 6.6 
                 31.1 
               
               
                   
                 Mean 
                 137.7 
                 2.0 
                 172.2 
                 7.2 
                 24.3 
               
               
                   
                 STDER 
                 40.9 
                 0.0 
                 31.5 
                 0.3 
                 5.0 
               
               
                 PBMC +  
                 1st 
                 10850.5 
                 2.0 
                 3125.9 
                 6.1 
                 512.4 
               
               
                 LPS(5 ug/ml) 
                 2nd 
                 10117.0 
                 2.0 
                 2900.2 
                 5.8 
                 500.0 
               
               
                   
                 3rd 
                 10150.0 
                 2.0 
                 2910.1 
                 5.5 
                 529.1 
               
               
                   
                 Mean 
                 10372.5 
                 2.0 
                 2978.7 
                 5.8 
                 513.9 
               
               
                   
                 STDER 
                 239.2 
                 0.0 
                 73.7 
                 0.2 
                 8.4 
               
               
                 PBMC +  
                 1st 
                 4825.5 
                 2.0 
                 1522.2 
                 5.2 
                 292.7 
               
               
                 YY101  
                 2nd 
                 16019.0 
                 2.0 
                 5242.2 
                 4.8 
                 1092.1 
               
               
                 0.01 uM 
                 Mean 
                 10422.3 
                 2.0 
                 3382.2 
                 5.0 
                 692.4 
               
               
                   
                 STDER 
                 5596.8 
                 0.0 
                 1860.0 
                 0.2 
                 399.7 
               
               
                 PBMC +  
                 1st 
                 4052.5 
                 2.0 
                 1338.6 
                 4.4 
                 304.2 
               
               
                 YY101 0.1 uM 
                 2nd 
                 9626.0 
                 2.0 
                 2755.6 
                 5.8 
                 475.1 
               
               
                   
                 Mean 
                 6839.3 
                 2.0 
                 2047.1 
                 5.1 
                 389.7 
               
               
                   
                 STDER 
                 2786.8 
                 0.0 
                 708.5 
                 0.7 
                 85.4 
               
               
                 PBMC +  
                 1st 
                 4611.0 
                 2.0 
                 1471.3 
                 5.5 
                 267.5 
               
               
                 YY101 1 uM 
                 2nd 
                 7230.0 
                 2.0 
                 2108.8 
                 5.5 
                 383.4 
               
               
                   
                 Mean 
                 5920.5 
                 2.0 
                 1790.1 
                 5.5 
                 325.5 
               
               
                   
                 STDER 
                 1309.5 
                 0.0 
                 318.8 
                 0.0 
                 58.0 
               
               
                 PBMC +  
                 1st 
                 22755.0 
                 2.0 
                 12584.2 
                 6.4 
                 1966.3 
               
               
                 YY101 10 uM 
                 2nd 
                 10032.5 
                 2.0 
                 2874.9 
                 6.1 
                 471.3 
               
               
                   
                 Mean 
                 16393.8 
                 2.0 
                 7729.5 
                 6.3 
                 1218.8 
               
               
                   
                 STDER 
                 6361.3 
                 0.0 
                 4854.6 
                 0.2 
                 747.5 
               
               
                 PBMC +  
                 1st 
                 4286.0 
                 2.0 
                 1394.1 
                 6.4 
                 217.8 
               
               
                 YY101 100 uM 
                 2nd 
                 1867.5 
                 2.0 
                 796.7 
                 7.0 
                 113.8 
               
               
                   
                 Mean 
                 3076.8 
                 2.0 
                 1095.4 
                 6.7 
                 165.8 
               
               
                   
                 STDER 
                 1209.3 
                 0.0 
                 298.7 
                 0.3 
                 52.0 
               
               
                 PBMC +  
                 1st 
                 14536.5 
                 2.0 
                 4509.4 
                 6.2 
                 727.3 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 13875.0 
                 2.0 
                 4222.1 
                 7.6 
                 555.5 
               
               
                 Xiidra  
                 Mean 
                 14205.8 
                 2.0 
                 4365.8 
                 6.9 
                 641.4 
               
               
                 0.001 uM 
                 STDER 
                 330.8 
                 0.0 
                 143.7 
                 0.7 
                 85.9 
               
               
                 PBMC +  
                 1st 
                 12993.5 
                 2.0 
                 3869.5 
                 5.2 
                 744.1 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 14776.0 
                 2.0 
                 4619.0 
                 5.8 
                 796.4 
               
               
                 Xiidra 0.01 uM 
                 Mean 
                 13884.8 
                 2.0 
                 4244.3 
                 5.5 
                 770.3 
               
               
                   
                 STDER 
                 891.3 
                 0.0 
                 374.7 
                 0.3 
                 26.1 
               
               
                 PBMC +  
                 1st 
                 10785.0 
                 2.0 
                 3105.2 
                 6.6 
                 470.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 10732.0 
                 2.0 
                 3088.6 
                 5.7 
                 541.9 
               
               
                 Xiidra 0.1 uM 
                 Mean 
                 10758.5 
                 2.0 
                 3096.9 
                 6.2 
                 506.2 
               
               
                   
                 STDER 
                 26.5 
                 0.0 
                 8.3 
                 0.5 
                 35.7 
               
               
                 PBMC +  
                 1st 
                 10830.0 
                 2.0 
                 3119.4 
                 4.8 
                 649.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 11231.0 
                 2.0 
                 3248.1 
                 4.4 
                 738.2 
               
               
                 Xiidra 1 uM 
                 Mean 
                 11030.5 
                 2.0 
                 3183.8 
                 4.6 
                 694.0 
               
               
                   
                 STDER 
                 200.5 
                 0.0 
                 64.4 
                 0.2 
                 44.2 
               
               
                 PBMC +  
                 1st 
                 8984.0 
                 2.0 
                 2573.5 
                 5.8 
                 443.7 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 10594.0 
                 2.0 
                 3045.6 
                 6.4 
                 475.9 
               
               
                 Xiidra 10 uM 
                 Mean 
                 9789.0 
                 2.0 
                 2809.5 
                 6.1 
                 459.8 
               
               
                   
                 STDER 
                 805.0 
                 0.0 
                 236.0 
                 0.3 
                 16.1 
               
               
                 PBMC +  
                 1st 
                 8030.0 
                 2.0 
                 2315.5 
                 7.1 
                 326.1 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 8710.5 
                 2.0 
                 2498.1 
                 5.8 
                 430.7 
               
               
                 Xiidra 100 uM 
                 Mean 
                 8370.3 
                 2.0 
                 2406.8 
                 6.5 
                 378.4 
               
               
                   
                 STDER 
                 340.3 
                 0.0 
                 91.3 
                 0.7 
                 52.3 
               
               
                 PBMC +  
                 1st 
                 12717.0 
                 2.0 
                 3765.3 
                 5.7 
                 660.6 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 12912.0 
                 2.0 
                 3838.5 
                 7.1 
                 540.6 
               
               
                 YY101  
                 3rd 
                 13837.0 
                 2.0 
                 4206.2 
                 6.6 
                 637.3 
               
               
                 0.001 uM 
                 Mean 
                 13155.3 
                 2.0 
                 3936.7 
                 6.5 
                 612.8 
               
               
                   
                 STDER 
                 345.5 
                 0.0 
                 136.4 
                 0.4 
                 36.7 
               
               
                 PBMC +  
                 1st 
                 13686.0 
                 2.0 
                 4143.7 
                 6.4 
                 647.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 13444.5 
                 2.0 
                 4045.9 
                 6.0 
                 674.3 
               
               
                 YY101  
                 3rd 
                 13067.0 
                 2.0 
                 3897.7 
                 6.1 
                 639.0 
               
               
                 0.01 uM 
                 Mean 
                 13399.2 
                 2.0 
                 4029.1 
                 6.2 
                 653.6 
               
               
                   
                 STDER 
                 180.1 
                 0.0 
                 71.5 
                 0.1 
                 10.7 
               
               
                 PBMC +  
                 1st 
                 12267.0 
                 2.0 
                 3601.3 
                 6.2 
                 580.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 12562.0 
                 2.0 
                 3708.0 
                 5.2 
                 713.1 
               
               
                 YY101 0.1 uM 
                 3rd 
                 11811.0 
                 2.0 
                 3442.0 
                 6.2 
                 555.2 
               
               
                   
                 Mean 
                 12213.3 
                 2.0 
                 3583.8 
                 5.9 
                 616.4 
               
               
                   
                 STDER 
                 218.4 
                 0.0 
                 77.3 
                 0.3 
                 48.9 
               
               
                 PBMC +  
                 1st 
                 13078.5 
                 2.0 
                 3902.2 
                 6.3 
                 619.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 13103.0 
                 2.0 
                 3911.6 
                 7.1 
                 550.9 
               
               
                 YY101 1 uM 
                 3rd 
                 13960.0 
                 2.0 
                 4257.8 
                 6.2 
                 686.7 
               
               
                   
                 Mean 
                 13380.5 
                 2.0 
                 4023.9 
                 6.5 
                 619.0 
               
               
                   
                 STDER 
                 289.8 
                 0.0 
                 117.0 
                 0.3 
                 39.2 
               
               
                 PBMC +  
                 1st 
                 11415.0 
                 2.0 
                 3308.6 
                 6.0 
                 551.4 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 12533.0 
                 2.0 
                 3697.4 
                 6.4 
                 577.7 
               
               
                 YY101 10 uM 
                 3rd 
                 11609.0 
                 2.0 
                 3373.4 
                 7.6 
                 443.9 
               
               
                   
                 Mean 
                 11852.3 
                 2.0 
                 3459.8 
                 6.7 
                 524.3 
               
               
                   
                 STDER 
                 344.9 
                 0.0 
                 120.3 
                 0.5 
                 40.9 
               
               
                 PBMC +  
                 1st 
                 992.0 
                 2.0 
                 541.0 
                 6.3 
                 85.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 1275.0 
                 2.0 
                 629.1 
                 6.2 
                 101.5 
               
               
                 YY101 100 uM 
                 3rd 
                 1263.0 
                 2.0 
                 625.5 
                 5.0 
                 125.1 
               
               
                   
                 Mean 
                 1176.7 
                 2.0 
                 598.6 
                 5.8 
                 104.2 
               
               
                   
                 STDER 
                 92.4 
                 0.0 
                 28.8 
                 0.4 
                 11.4 
               
               
                 PBMC +  
                 1st 
                 6216.5 
                 2.0 
                 1856.7 
                 4.5 
                 412.6 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 6415.5 
                 2.0 
                 1905.5 
                 5.8 
                 328.5 
               
               
                 YDE011  
                 3rd 
                 6318.0 
                 2.0 
                 1881.6 
                 5.8 
                 324.4 
               
               
                 0.001 uM 
                 Mean 
                 6316.7 
                 2.0 
                 1881.3 
                 5.4 
                 355.2 
               
               
                   
                 STDER 
                 57.5 
                 0.0 
                 14.1 
                 0.4 
                 28.7 
               
               
                 PBMC +  
                 1st 
                 6375.0 
                 2.0 
                 1895.6 
                 6.3 
                 300.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 7050.0 
                 2.0 
                 2063.3 
                 6.2 
                 332.8 
               
               
                 YDE011  
                 3rd 
                 6660.0 
                 2.0 
                 1965.9 
                 5.1 
                 385.5 
               
               
                 0.01 uM 
                 Mean 
                 6695.0 
                 2.0 
                 1974.9 
                 5.9 
                 339.7 
               
               
                   
                 STDER 
                 195.6 
                 0.0 
                 48.6 
                 0.4 
                 24.7 
               
               
                 PBMC +  
                 1st 
                 5566.0 
                 2.0 
                 1699.1 
                 6.6 
                 257.4 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 5817.0 
                 2.0 
                 1759.6 
                 5.0 
                 351.9 
               
               
                 YDE011  
                 3rd 
                 5428.0 
                 2.0 
                 1666.0 
                 5.7 
                 292.3 
               
               
                 0.1 uM 
                 Mean 
                 5603.7 
                 2.0 
                 1708.3 
                 5.8 
                 300.6 
               
               
                   
                 STDER 
                 113.9 
                 0.0 
                 27.4 
                 0.5 
                 27.6 
               
               
                 PBMC +  
                 1st 
                 5965.5 
                 2.0 
                 1795.6 
                 5.5 
                 326.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 6315.0 
                 2.0 
                 1880.8 
                 4.8 
                 391.8 
               
               
                 YDE011 1 uM 
                 3rd 
                 5630.0 
                 2.0 
                 1714.5 
                 4.8 
                 357.2 
               
               
                   
                 Mean 
                 5970.2 
                 2.0 
                 1797.0 
                 5.0 
                 358.5 
               
               
                   
                 STDER 
                 197.8 
                 0.0 
                 48.0 
                 0.2 
                 18.9 
               
               
                   
                 1st 
                 4743.0 
                 2.0 
                 1502.6 
                 5.4 
                 278.3 
               
               
                 PBMC +  
                 2nd 
                 5215.5 
                 2.0 
                 1615.2 
                 5.8 
                 278.5 
               
               
                 LPS(5 ug/ml) + 
                 3rd 
                 5049.0 
                 2.0 
                 1575.4 
                 4.7 
                 335.2 
               
               
                 YDE011  
                 Mean 
                 5002.5 
                 2.0 
                 1564.4 
                 5.3 
                 297.3 
               
               
                 10 uM 
                 STDER 
                 138.4 
                 0.0 
                 33.0 
                 0.3 
                 18.9 
               
               
                 PBMC +  
                 1st 
                 262.0 
                 2.0 
                 252.0 
                 6.1 
                 41.3 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 360.0 
                 2.0 
                 301.1 
                 6.1 
                 49.4 
               
               
                 YDE011  
                 3rd 
                 342.0 
                 2.0 
                 292.6 
                 6.0 
                 48.8 
               
               
                 100 uM 
                 Mean 
                 321.3 
                 2.0 
                 281.9 
                 6.1 
                 46.5 
               
               
                   
                 STDER 
                 30.1 
                 0.0 
                 15.2 
                 0.0 
                 2.6 
               
               
                 PBMC +  
                 1st 
                 7127.0 
                 2.0 
                 2082.8 
                 4.4 
                 473.4 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 7646.5 
                 2.0 
                 2215.5 
                 6.0 
                 369.2 
               
               
                 YDE043  
                 3rd 
                 6916.0 
                 2.0 
                 2029.7 
                 4.6 
                 441.2 
               
               
                 0.001 uM 
                 Mean 
                 7229.8 
                 2.0 
                 2109.3 
                 5.0 
                 427.9 
               
               
                   
                 STDER 
                 217.1 
                 0.0 
                 55.2 
                 0.5 
                 30.8 
               
               
                 PBMC +  
                 1st 
                 6942.0 
                 2.0 
                 2036.2 
                 4.5 
                 452.5 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 6650.0 
                 2.0 
                 1963.4 
                 6.6 
                 297.5 
               
               
                 YDE043  
                 3rd 
                 7082.0 
                 2.0 
                 2071.4 
                 5.0 
                 414.3 
               
               
                 0.01 uM 
                 Mean 
                 6891.3 
                 2.0 
                 2023.7 
                 5.4 
                 388.1 
               
               
                   
                 STDER 
                 127.3 
                 0.0 
                 31.8 
                 0.6 
                 46.6 
               
               
                 PBMC +  
                 1st 
                 7234.0 
                 2.0 
                 2109.9 
                 5.0 
                 422.0 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 5852.5 
                 2.0 
                 1768.2 
                 5.6 
                 315.8 
               
               
                 YDE043  
                 3rd 
                 6350.5 
                 2.0 
                 1889.5 
                 5.6 
                 337.4 
               
               
                 0.1 uM 
                 Mean 
                 6479.0 
                 2.0 
                 1922.5 
                 5.4 
                 358.4 
               
               
                   
                 STDER 
                 403.9 
                 0.0 
                 100.0 
                 0.2 
                 32.4 
               
               
                 PBMC +  
                 1st 
                 7486.0 
                 2.0 
                 2174.1 
                 5.8 
                 374.9 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 7253.0 
                 2.0 
                 2114.7 
                 4.8 
                 440.6 
               
               
                 YDE043 1 uM 
                 3rd 
                 7122.0 
                 2.0 
                 2081.5 
                 5.3 
                 392.7 
               
               
                   
                 Mean 
                 7287.0 
                 2.0 
                 2123.4 
                 5.3 
                 402.7 
               
               
                   
                 STDER 
                 106.4 
                 0.0 
                 27.1 
                 0.3 
                 19.6 
               
               
                 PBMC +  
                 1st 
                 6348.5 
                 2.0 
                 1889.0 
                 5.2 
                 363.3 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 5845.0 
                 2.0 
                 1766.4 
                 5.4 
                 327.1 
               
               
                 YDE043  
                 3rd 
                 5753.0 
                 2.0 
                 1744.2 
                 5.6 
                 311.5 
               
               
                 10 uM 
                 Mean 
                 5982.2 
                 2.0 
                 1799.9 
                 5.4 
                 333.9 
               
               
                   
                 STDER 
                 185.1 
                 0.0 
                 45.0 
                 0.1 
                 15.3 
               
               
                 PBMC +  
                 1st 
                 275.0 
                 2.0 
                 258.9 
                 6.2 
                 41.8 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 364.0 
                 2.0 
                 303.0 
                 6.4 
                 47.3 
               
               
                 YDE043  
                 Mean 
                 319.5 
                 2.0 
                 281.0 
                 6.3 
                 44.6 
               
               
                 100 uM 
                 STDER 
                 44.5 
                 0.0 
                 22.1 
                 0.1 
                 2.8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 36 
               
             
            
               
                   
               
               
                 Bead name: Fas Ligand 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Con- 
                 Live  
                 conc/  
               
               
                   
                   
                   
                   
                 cen- 
                 cell 
                 live 
               
               
                   
                   
                   
                 Dilu- 
                 tration 
                 (10{circumflex over ( )}5/ 
                 cell (pg/ 
               
               
                 Sample 
                   
                 MFI 
                 tion 
                 (pg/ml) 
                 ml) 
                 10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.0 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC +  
                 1st 
                 4.0 
                 2.0 
                 4.6 
                 7.5 
                 0.6 
               
               
                 0.5% DMSO 
                 2nd 
                 4.0 
                 2.0 
                 4.6 
                 7.4 
                 0.6 
               
               
                   
                 3rd 
                 4.0 
                 2.0 
                 4.6 
                 6.6 
                 0.7 
               
               
                   
                 Mean 
                 4.0 
                 2.0 
                 4.6 
                 7.2 
                 0.6 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.3 
                 0.0 
               
               
                 PBMC +  
                 1st 
                 32.0 
                 2.0 
                 23.1 
                 6.1 
                 3.8 
               
               
                 LPS(5 ug/ml) 
                 2nd 
                 47.0 
                 2.0 
                 33.4 
                 5.8 
                 5.8 
               
               
                   
                 3rd 
                 45.0 
                 2.0 
                 32.0 
                 5.5 
                 5.8 
               
               
                   
                 Mean 
                 41.3 
                 2.0 
                 29.5 
                 5.8 
                 5.1 
               
               
                   
                 STDER 
                 4.7 
                 0.0 
                 3.2 
                 0.2 
                 0.7 
               
               
                 PBMC + YY101  
                 1st 
                 7.0 
                 2.0 
                 6.5 
                 5.2 
                 1.3 
               
               
                 0.01 uM 
                 2nd 
                 10.5 
                 2.0 
                 8.8 
                 4.8 
                 1.8 
               
               
                   
                 Mean 
                 8.8 
                 2.0 
                 7.7 
                 5.0 
                 1.5 
               
               
                   
                 STDER 
                 1.8 
                 0.0 
                 1.1 
                 0.2 
                 0.3 
               
               
                 PBMC + YY101  
                 1st 
                 7.0 
                 2.0 
                 6.5 
                 4.4 
                 1.5 
               
               
                 0.1 uM 
                 2nd 
                 9.0 
                 2.0 
                 7.8 
                 5.8 
                 1.3 
               
               
                   
                 Mean 
                 8.0 
                 2.0 
                 7.2 
                 5.1 
                 1.4 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 0.6 
                 0.7 
                 0.1 
               
               
                 PBMC + YY101  
                 1st 
                 7.0 
                 2.0 
                 6.5 
                 5.5 
                 1.2 
               
               
                 1 uM 
                 2nd 
                 10.0 
                 2.0 
                 8.5 
                 5.5 
                 1.5 
               
               
                   
                 Mean 
                 8.5 
                 2.0 
                 7.5 
                 5.5 
                 1.4 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 1.0 
                 0.0 
                 0.2 
               
               
                 PBMC + YY101  
                 1st 
                 18.5 
                 2.0 
                 14.0 
                 6.4 
                 2.2 
               
               
                 10 uM 
                 2nd 
                 16.0 
                 2.0 
                 12.4 
                 6.1 
                 2.0 
               
               
                   
                 Mean 
                 17.3 
                 2.0 
                 13.2 
                 6.3 
                 2.1 
               
               
                   
                 STDER 
                 1.3 
                 0.0 
                 0.8 
                 0.2 
                 0.1 
               
               
                 PBMC + YY101  
                 1st 
                 9.0 
                 2.0 
                 7.8 
                 6.4 
                 1.2 
               
               
                 100 uM 
                 2nd 
                 6.0 
                 2.0 
                 5.9 
                 7.0 
                 0.8 
               
               
                   
                 Mean 
                 7.5 
                 2.0 
                 6.9 
                 6.7 
                 1.0 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 1.0 
                 0.3 
                 0.2 
               
               
                 PBMC +  
                 1st 
                 77.0 
                 2.0 
                 54.4 
                 6.2 
                 8.8 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 45.0 
                 2.0 
                 32.0 
                 7.6 
                 4.2 
               
               
                 Xiidra 0.001 uM 
                 Mean 
                 61.0 
                 2.0 
                 43.2 
                 6.9 
                 6.5 
               
               
                   
                 STDER 
                 16.0 
                 0.0 
                 11.2 
                 0.7 
                 2.3 
               
               
                 PBMC +  
                 1st 
                 56.0 
                 2.0 
                 39.7 
                 5.2 
                 7.6 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 41.0 
                 2.0 
                 29.3 
                 5.8 
                 5.0 
               
               
                 Xiidra 0.01 uM 
                 Mean 
                 48.5 
                 2.0 
                 34.5 
                 5.5 
                 6.3 
               
               
                   
                 STDER 
                 7.5 
                 0.0 
                 5.2 
                 0.3 
                 1.3 
               
               
                 PBMC +  
                 1st 
                 36.5 
                 2.0 
                 26.2 
                 6.6 
                 4.0 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 37.5 
                 2.0 
                 26.9 
                 5.7 
                 4.7 
               
               
                 Xiidra 0.1 uM 
                 Mean 
                 37.0 
                 2.0 
                 26.5 
                 6.2 
                 4.3 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 0.3 
                 0.5 
                 0.4 
               
               
                 PBMC +  
                 1st 
                 37.0 
                 2.0 
                 26.5 
                 4.8 
                 5.5 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 24.5 
                 2.0 
                 18.1 
                 4.4 
                 4.1 
               
               
                 Xiidra 1 uM 
                 Mean 
                 30.8 
                 2.0 
                 22.3 
                 4.6 
                 4.8 
               
               
                   
                 STDER 
                 6.3 
                 0.0 
                 4.2 
                 0.2 
                 0.7 
               
               
                 PBMC +  
                 1st 
                 34.0 
                 2.0 
                 24.5 
                 5.8 
                 4.2 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 44.5 
                 2.0 
                 31.7 
                 6.4 
                 5.0 
               
               
                 Xiidra 10 uM 
                 Mean 
                 39.3 
                 2.0 
                 28.1 
                 6.1 
                 4.6 
               
               
                   
                 STDER 
                 5.3 
                 0.0 
                 3.6 
                 0.3 
                 0.4 
               
               
                 PBMC +  
                 1st 
                 42.0 
                 2.0 
                 30.0 
                 7.1 
                 4.2 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 40.0 
                 2.0 
                 28.6 
                 5.8 
                 4.9 
               
               
                 Xiidra 100 uM 
                 Mean 
                 41.0 
                 2.0 
                 29.3 
                 6.5 
                 4.6 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 0.7 
                 0.7 
                 0.4 
               
               
                 PBMC +  
                 1st 
                 50.5 
                 2.0 
                 35.8 
                 5.7 
                 6.3 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 46.0 
                 2.0 
                 32.7 
                 7.1 
                 4.6 
               
               
                 YY101  
                 3rd 
                 33.5 
                 2.0 
                 24.1 
                 6.6 
                 3.7 
               
               
                 0.001 uM 
                 Mean 
                 43.3 
                 2.0 
                 30.9 
                 6.5 
                 4.9 
               
               
                   
                 STDER 
                 5.1 
                 0.0 
                 3.5 
                 0.4 
                 0.8 
               
               
                 PBMC +  
                 1st 
                 43.0 
                 2.0 
                 30.7 
                 6.4 
                 4.8 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 55.5 
                 2.0 
                 39.3 
                 6.0 
                 6.6 
               
               
                 YY101 0.01 uM 
                 3rd 
                 44.0 
                 2.0 
                 31.3 
                 6.1 
                 5.1 
               
               
                   
                 Mean 
                 47.5 
                 2.0 
                 33.8 
                 6.2 
                 5.5 
               
               
                   
                 STDER 
                 4.0 
                 0.0 
                 2.8 
                 0.1 
                 0.5 
               
               
                 PBMC +  
                 1st 
                 46.0 
                 2.0 
                 32.7 
                 6.2 
                 5.3 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 43.0 
                 2.0 
                 30.7 
                 5.2 
                 5.9 
               
               
                 YY101 0.1 uM 
                 3rd 
                 35.0 
                 2.0 
                 25.2 
                 6.2 
                 4.1 
               
               
                   
                 Mean 
                 41.3 
                 2.0 
                 29.5 
                 5.9 
                 5.1 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 2.3 
                 0.3 
                 0.5 
               
               
                 PBMC +  
                 1st 
                 50.0 
                 2.0 
                 35.5 
                 6.3 
                 5.6 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 51.0 
                 2.0 
                 36.2 
                 7.1 
                 5.1 
               
               
                 YY101 1 uM 
                 3rd 
                 44.5 
                 2.0 
                 31.7 
                 6.2 
                 5.1 
               
               
                   
                 Mean 
                 48.5 
                 2.0 
                 34.5 
                 6.5 
                 5.3 
               
               
                   
                 STDER 
                 2.0 
                 0.0 
                 1.4 
                 0.3 
                 0.2 
               
               
                 PBMC +  
                 1st 
                 82.0 
                 2.0 
                 58.0 
                 6.0 
                 9.7 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 76.0 
                 2.0 
                 53.7 
                 6.4 
                 8.4 
               
               
                 YY101 10 uM 
                 3rd 
                 40.0 
                 2.0 
                 28.6 
                 7.6 
                 3.8 
               
               
                   
                 Mean 
                 66.0 
                 2.0 
                 46.7 
                 6.7 
                 7.3 
               
               
                   
                 STDER 
                 13.1 
                 0.0 
                 9.2 
                 0.5 
                 1.8 
               
               
                 PBMC +  
                 1st 
                 55.0 
                 2.0 
                 39.0 
                 6.3 
                 6.2 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 21.5 
                 2.0 
                 16.0 
                 6.2 
                 2.6 
               
               
                 YY101 100 uM 
                 3rd 
                 29.0 
                 2.0 
                 21.1 
                 5.0 
                 4.2 
               
               
                   
                 Mean 
                 35.2 
                 2.0 
                 25.4 
                 5.8 
                 4.3 
               
               
                   
                 STDER 
                 10.2 
                 0.0 
                 7.0 
                 0.4 
                 1.0 
               
               
                 PBMC +  
                 1st 
                 21.0 
                 2.0 
                 15.7 
                 4.5 
                 3.5 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 25.0 
                 2.0 
                 18.4 
                 5.8 
                 3.2 
               
               
                 YDE011 0.001  
                 3rd 
                 22.0 
                 2.0 
                 16.4 
                 5.8 
                 2.8 
               
               
                 uM 
                 Mean 
                 22.7 
                 2.0 
                 16.8 
                 5.4 
                 3.2 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 0.8 
                 0.4 
                 0.2 
               
               
                 PBMC +  
                 1st 
                 23.0 
                 2.0 
                 17.1 
                 6.3 
                 2.7 
               
               
                 LPS(5 ug/ml) + 
                 2nd 
                 18.0 
                 2.0 
                 13.7 
                 6.2 
                 2.2 
               
               
                 YDE011  
                 3rd 
                 21.0 
                 2.0 
                 15.7 
                 5.1 
                 3.1 
               
               
                 0.01 uM 
                 Mean 
                 20.7 
                 2.0 
                 15.5 
                 5.9 
                 2.7 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 1.0 
                 0.4 
                 0.3 
               
               
                 PBMC +  
                 1st 
                 26.0 
                 2.0 
                 19.1 
                 6.6 
                 2.9 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 24.0 
                 2.0 
                 17.7 
                 5.0 
                 3.5 
               
               
                 YDE011 0.1 uM 
                 3rd 
                 20.0 
                 2.0 
                 15.0 
                 5.7 
                 2.6 
               
               
                   
                 Mean 
                 23.3 
                 2.0 
                 17.3 
                 5.8 
                 3.0 
               
               
                   
                 STDER 
                 1.8 
                 0.0 
                 1.2 
                 0.5 
                 0.3 
               
               
                 PBMC +  
                 1st 
                 23.5 
                 2.0 
                 17.4 
                 5.5 
                 3.2 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 20.5 
                 2.0 
                 15.4 
                 4.8 
                 3.2 
               
               
                 YDE011 1 uM 
                 3rd 
                 20.0 
                 2.0 
                 15.0 
                 4.8 
                 3.1 
               
               
                   
                 Mean 
                 21.3 
                 2.0 
                 15.9 
                 5.0 
                 3.2 
               
               
                   
                 STDER 
                 1.1 
                 0.0 
                 0.7 
                 0.2 
                 0.0 
               
               
                 PBMC +  
                 1st 
                 27.0 
                 2.0 
                 19.7 
                 5.4 
                 3.7 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 24.0 
                 2.0 
                 17.7 
                 5.8 
                 3.1 
               
               
                 YDE011 10 uM 
                 3rd 
                 19.0 
                 2.0 
                 14.4 
                 4.7 
                 3.1 
               
               
                   
                 Mean 
                 23.3 
                 2.0 
                 17.3 
                 5.3 
                 3.3 
               
               
                   
                 STDER 
                 2.3 
                 0.0 
                 1.6 
                 0.3 
                 0.2 
               
               
                 PBMC +  
                 1st 
                 15.0 
                 2.0 
                 11.7 
                 6.1 
                 1.9 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 17.0 
                 2.0 
                 13.1 
                 6.1 
                 2.1 
               
               
                 YDE011  
                 3rd 
                 20.0 
                 2.0 
                 15.0 
                 6.0 
                 2.5 
               
               
                 100 uM 
                 Mean 
                 17.3 
                 2.0 
                 13.3 
                 6.1 
                 2.2 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 1.0 
                 0.0 
                 0.2 
               
               
                 PBMC +  
                 1st 
                 23.0 
                 2.0 
                 17.1 
                 4.4 
                 3.9 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 28.0 
                 2.0 
                 20.4 
                 6.0 
                 3.4 
               
               
                 YDE043  
                 3rd 
                 17.0 
                 2.0 
                 13.1 
                 4.6 
                 2.8 
               
               
                 0.001 uM 
                 Mean 
                 22.7 
                 2.0 
                 16.8 
                 5.0 
                 3.4 
               
               
                   
                 STDER 
                 3.2 
                 0.0 
                 2.1 
                 0.5 
                 0.3 
               
               
                 PBMC +  
                 1st 
                 26.0 
                 2.0 
                 19.1 
                 4.5 
                 4.2 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 21.0 
                 2.0 
                 15.7 
                 6.6 
                 2.4 
               
               
                 YDE043  
                 3rd 
                 43.5 
                 2.0 
                 31.0 
                 5.0 
                 6.2 
               
               
                 0.01 uM 
                 Mean 
                 30.2 
                 2.0 
                 21.9 
                 5.4 
                 4.3 
               
               
                   
                 STDER 
                 6.8 
                 0.0 
                 4.6 
                 0.6 
                 1.1 
               
               
                 PBMC +  
                 1st 
                 19.0 
                 2.0 
                 14.4 
                 5.0 
                 2.9 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 16.5 
                 2.0 
                 12.7 
                 5.6 
                 2.3 
               
               
                 YDE043 0.1 uM 
                 3rd 
                 16.0 
                 2.0 
                 12.4 
                 5.6 
                 2.2 
               
               
                   
                 Mean 
                 17.2 
                 2.0 
                 13.2 
                 5.4 
                 2.5 
               
               
                   
                 STDER 
                 0.9 
                 0.0 
                 0.6 
                 0.2 
                 0.2 
               
               
                 PBMC +  
                 1st 
                 18.0 
                 2.0 
                 13.7 
                 5.8 
                 2.4 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 21.0 
                 2.0 
                 15.7 
                 4.8 
                 3.3 
               
               
                 YDE043 1 uM 
                 3rd 
                 28.0 
                 2.0 
                 20.4 
                 5.3 
                 3.9 
               
               
                   
                 Mean 
                 22.3 
                 2.0 
                 16.6 
                 5.3 
                 3.2 
               
               
                   
                 STDER 
                 3.0 
                 0.0 
                 2.0 
                 0.3 
                 0.4 
               
               
                 PBMC +  
                 1st 
                 22.0 
                 2.0 
                 16.4 
                 5.2 
                 3.2 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 23.0 
                 2.0 
                 17.1 
                 5.4 
                 3.2 
               
               
                 YDE043 10 uM 
                 3rd 
                 21.0 
                 2.0 
                 15.7 
                 5.6 
                 2.8 
               
               
                   
                 Mean 
                 22.0 
                 2.0 
                 16.4 
                 5.4 
                 3.0 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 0.4 
                 0.1 
                 0.1 
               
               
                 PBMC +  
                 1st 
                 20.0 
                 2.0 
                 15.0 
                 6.2 
                 2.4 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 14.5 
                 2.0 
                 11.4 
                 6.4 
                 1.8 
               
               
                 YDE043  
                 Mean 
                 17.3 
                 2.0 
                 13.2 
                 6.3 
                 2.1 
               
               
                 100 uM 
                 STDER 
                 2.8 
                 0.0 
                 1.8 
                 0.1 
                 0.3 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 37 
               
             
            
               
                   
               
               
                 Bead name: CCL20/MIP-3 alpha 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Con- 
                 Live  
                 conc/  
               
               
                   
                   
                   
                   
                 cen- 
                 cell 
                 live 
               
               
                   
                   
                   
                 Dilu- 
                 tration 
                 (10{circumflex over ( )}5/ 
                 cell (pg/ 
               
               
                 Sample 
                   
                 MFI 
                 tion 
                 (pg/ml) 
                 ml) 
                 10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.5 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 −0.5 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC +  
                 1st 
                 3.5 
                 2.0 
                   
                 7.5 
                 0.0 
               
               
                 0.5% DMSO 
                 2nd 
                 2.0 
                 2.0 
                   
                 7.4 
                 0.0 
               
               
                   
                 3rd 
                 3.5 
                 2.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 Mean 
                 3.0 
                 2.0 
                 #DIV/0! 
                 7.2 
                 0.0 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC +  
                 1st 
                 432.0 
                 2.0 
                 384.5 
                 6.1 
                 63.0 
               
               
                 LPS(5 ug/ml) 
                 2nd 
                 390.5 
                 2.0 
                 351.1 
                 5.8 
                 60.5 
               
               
                   
                 3rd 
                 428.5 
                 2.0 
                 381.7 
                 5.5 
                 69.4 
               
               
                   
                 Mean 
                 417.0 
                 2.0 
                 372.4 
                 5.8 
                 64.3 
               
               
                   
                 STDER 
                 13.3 
                 0.0 
                 10.7 
                 0.2 
                 2.6 
               
               
                 PBMC + YY101  
                 1st 
                 7.5 
                 2.0 
                 7.3 
                 5.2 
                 1.4 
               
               
                 0.01 uM 
                 2nd 
                 14.5 
                 2.0 
                 16.8 
                 4.8 
                 3.5 
               
               
                   
                 Mean 
                 11.0 
                 2.0 
                 12.1 
                 5.0 
                 2.5 
               
               
                   
                 STDER 
                 3.5 
                 0.0 
                 4.7 
                 0.2 
                 1.0 
               
               
                 PBMC +  
                 1st 
                 7.5 
                 2.0 
                 7.3 
                 4.4 
                 1.7 
               
               
                 YY101 0.1 uM 
                 2nd 
                 10.5 
                 2.0 
                 11.6 
                 5.8 
                 2.0 
               
               
                   
                 Mean 
                 9.0 
                 2.0 
                 9.5 
                 5.1 
                 1.8 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 2.1 
                 0.7 
                 0.2 
               
               
                 PBMC +  
                 1st 
                 7.5 
                 2.0 
                 7.3 
                 5.5 
                 1.3 
               
               
                 YY101 1 uM 
                 2nd 
                 9.5 
                 2.0 
                 10.2 
                 5.5 
                 1.9 
               
               
                   
                 Mean 
                 8.5 
                 2.0 
                 8.8 
                 5.5 
                 1.6 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 1.4 
                 0.0 
                 0.3 
               
               
                 PBMC +  
                 1st 
                 51.5 
                 2.0 
                 57.4 
                 6.4 
                 9.0 
               
               
                 YY101 10 uM 
                 2nd 
                 21.5 
                 2.0 
                 25.3 
                 6.1 
                 4.1 
               
               
                   
                 Mean 
                 36.5 
                 2.0 
                 41.3 
                 6.3 
                 6.6 
               
               
                   
                 STDER 
                 15.0 
                 0.0 
                 16.1 
                 0.2 
                 2.4 
               
               
                 PBMC +  
                 1st 
                 25.5 
                 2.0 
                 29.8 
                 6.4 
                 4.7 
               
               
                 YY101 100 uM 
                 2nd 
                 13.0 
                 2.0 
                 14.9 
                 7.0 
                 2.1 
               
               
                   
                 Mean 
                 19.3 
                 2.0 
                 22.4 
                 6.7 
                 3.4 
               
               
                   
                 STDER 
                 6.3 
                 0.0 
                 7.5 
                 0.3 
                 1.3 
               
               
                 PBMC +  
                 1st 
                 453.5 
                 2.0 
                 401.8 
                 6.2 
                 64.8 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 392.5 
                 2.0 
                 352.7 
                 7.6 
                 46.4 
               
               
                 Xiidra 0.001 uM 
                 Mean 
                 423.0 
                 2.0 
                 377.2 
                 6.9 
                 55.6 
               
               
                   
                 STDER 
                 30.5 
                 0.0 
                 24.5 
                 0.7 
                 9.2 
               
               
                 PBMC +  
                 1st 
                 485.5 
                 2.0 
                 427.3 
                 5.2 
                 82.2 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 377.5 
                 2.0 
                 340.5 
                 5.8 
                 58.7 
               
               
                 Xiidra 0.01 uM 
                 Mean 
                 431.5 
                 2.0 
                 383.9 
                 5.5 
                 70.4 
               
               
                   
                 STDER 
                 54.0 
                 0.0 
                 43.4 
                 0.3 
                 11.7 
               
               
                 PBMC +  
                 1st 
                 306.5 
                 2.0 
                 282.4 
                 6.6 
                 42.8 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 248.5 
                 2.0 
                 234.1 
                 5.7 
                 41.1 
               
               
                 Xiidra 0.1 uM 
                 Mean 
                 277.5 
                 2.0 
                 258.3 
                 6.2 
                 41.9 
               
               
                   
                 STDER 
                 29.0 
                 0.0 
                 24.2 
                 0.5 
                 0.9 
               
               
                 PBMC +  
                 1st 
                 459.5 
                 2.0 
                 406.6 
                 4.8 
                 84.7 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 258.5 
                 2.0 
                 242.5 
                 4.4 
                 55.1 
               
               
                 Xiidra 1 uM 
                 Mean 
                 359.0 
                 2.0 
                 324.5 
                 4.6 
                 69.9 
               
               
                   
                 STDER 
                 100.5 
                 0.0 
                 82.0 
                 0.2 
                 14.8 
               
               
                 PBMC +  
                 1st 
                 356.5 
                 2.0 
                 323.4 
                 5.8 
                 55.8 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 353.0 
                 2.0 
                 320.6 
                 6.4 
                 50.1 
               
               
                 Xiidra 10 uM 
                 Mean 
                 354.8 
                 2.0 
                 322.0 
                 6.1 
                 52.9 
               
               
                   
                 STDER 
                 1.8 
                 0.0 
                 1.4 
                 0.3 
                 2.8 
               
               
                 PBMC +  
                 1st 
                 228.5 
                 2.0 
                 217.2 
                 7.1 
                 30.6 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 253.0 
                 2.0 
                 237.9 
                 5.8 
                 41.0 
               
               
                 Xiidra 100 uM 
                 Mean 
                 240.8 
                 2.0 
                 227.6 
                 6.5 
                 35.8 
               
               
                   
                 STDER 
                 12.3 
                 0.0 
                 10.3 
                 0.7 
                 5.2 
               
               
                 PBMC +  
                 1st 
                 329.0 
                 2.0 
                 301.0 
                 5.7 
                 52.8 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 315.5 
                 2.0 
                 289.9 
                 7.1 
                 40.8 
               
               
                 YY101  
                 3rd 
                 395.5 
                 2.0 
                 355.1 
                 6.6 
                 53.8 
               
               
                 0.001 uM 
                 Mean 
                 346.7 
                 2.0 
                 315.3 
                 6.5 
                 49.1 
               
               
                   
                 STDER 
                 24.7 
                 0.0 
                 20.2 
                 0.4 
                 4.2 
               
               
                 PBMC +  
                 1st 
                 431.0 
                 2.0 
                 383.7 
                 6.4 
                 60.0 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 375.5 
                 2.0 
                 338.9 
                 6.0 
                 56.5 
               
               
                 YY101 0.01 uM 
                 3rd 
                 464.5 
                 2.0 
                 410.6 
                 6.1 
                 67.3 
               
               
                   
                 Mean 
                 423.7 
                 2.0 
                 377.7 
                 6.2 
                 61.2 
               
               
                   
                 STDER 
                 26.0 
                 0.0 
                 20.9 
                 0.1 
                 3.2 
               
               
                 PBMC +  
                 1st 
                 424.5 
                 2.0 
                 378.5 
                 6.2 
                 61.0 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 407.0 
                 2.0 
                 364.4 
                 5.2 
                 70.1 
               
               
                 YY101 0.1 uM 
                 3rd 
                 388.5 
                 2.0 
                 349.4 
                 6.2 
                 56.4 
               
               
                   
                 Mean 
                 406.7 
                 2.0 
                 364.1 
                 5.9 
                 62.5 
               
               
                   
                 STDER 
                 10.4 
                 0.0 
                 8.4 
                 0.3 
                 4.0 
               
               
                 PBMC +  
                 1st 
                 356.0 
                 2.0 
                 323.0 
                 6.3 
                 51.3 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 364.5 
                 2.0 
                 330.0 
                 7.1 
                 46.5 
               
               
                 YY101 1 uM 
                 3rd 
                 404.5 
                 2.0 
                 362.4 
                 6.2 
                 58.4 
               
               
                   
                 Mean 
                 375.0 
                 2.0 
                 338.5 
                 6.5 
                 52.1 
               
               
                   
                 STDER 
                 15.0 
                 0.0 
                 12.1 
                 0.3 
                 3.5 
               
               
                 PBMC +  
                 1st 
                 357.5 
                 2.0 
                 324.3 
                 6.0 
                 54.0 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 423.5 
                 2.0 
                 377.7 
                 6.4 
                 59.0 
               
               
                 YY101 10 uM 
                 3rd 
                 454.5 
                 2.0 
                 402.6 
                 7.6 
                 53.0 
               
               
                   
                 Mean 
                 411.8 
                 2.0 
                 368.2 
                 6.7 
                 55.3 
               
               
                   
                 STDER 
                 28.6 
                 0.0 
                 23.1 
                 0.5 
                 1.9 
               
               
                 PBMC +  
                 1st 
                 692.5 
                 2.0 
                 590.1 
                 6.3 
                 93.7 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 694.5 
                 2.0 
                 591.7 
                 6.2 
                 95.4 
               
               
                 YY101 100 uM 
                 3rd 
                 646.0 
                 2.0 
                 553.9 
                 5.0 
                 110.8 
               
               
                   
                 Mean 
                 677.7 
                 2.0 
                 578.6 
                 5.8 
                 100.0 
               
               
                   
                 STDER 
                 15.8 
                 0.0 
                 12.4 
                 0.4 
                 5.4 
               
               
                 PBMC +  
                 1st 
                 284.5 
                 2.0 
                 264.2 
                 4.5 
                 58.7 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 235.5 
                 2.0 
                 223.2 
                 5.8 
                 38.5 
               
               
                 YDE011  
                 3rd 
                 270.0 
                 2.0 
                 252.1 
                 5.8 
                 43.5 
               
               
                 0.001 uM 
                 Mean 
                 263.3 
                 2.0 
                 246.5 
                 5.4 
                 46.9 
               
               
                   
                 STDER 
                 14.5 
                 0.0 
                 12.2 
                 0.4 
                 6.1 
               
               
                 PBMC +  
                 1st 
                 247.5 
                 2.0 
                 233.3 
                 6.3 
                 37.0 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 278.0 
                 2.0 
                 258.8 
                 6.2 
                 41.7 
               
               
                 YDE011  
                 3rd 
                 297.0 
                 2.0 
                 274.6 
                 5.1 
                 53.8 
               
               
                 0.01 uM 
                 Mean 
                 274.2 
                 2.0 
                 255.6 
                 5.9 
                 44.2 
               
               
                   
                 STDER 
                 14.4 
                 0.0 
                 12.0 
                 0.4 
                 5.0 
               
               
                 PBMC +  
                 1st 
                 253.5 
                 2.0 
                 238.3 
                 6.6 
                 36.1 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 245.5 
                 2.0 
                 231.6 
                 5.0 
                 46.3 
               
               
                 YDE011 0.1 uM 
                 3rd 
                 265.5 
                 2.0 
                 248.4 
                 5.7 
                 43.6 
               
               
                   
                 Mean 
                 254.8 
                 2.0 
                 239.4 
                 5.8 
                 42.0 
               
               
                   
                 STDER 
                 5.8 
                 0.0 
                 4.9 
                 0.5 
                 3.1 
               
               
                 PBMC + 
                 1st 
                 212.5 
                 2.0 
                 203.6 
                 5.5 
                 37.0 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 218.5 
                 2.0 
                 208.7 
                 4.8 
                 43.5 
               
               
                 YDE011 1 uM 
                 3rd 
                 233.5 
                 2.0 
                 221.5 
                 4.8 
                 46.1 
               
               
                   
                 Mean 
                 221.5 
                 2.0 
                 211.3 
                 5.0 
                 42.2 
               
               
                   
                 STDER 
                 6.2 
                 0.0 
                 5.3 
                 0.2 
                 2.7 
               
               
                 PBMC +  
                 1st 
                 248.5 
                 2.0 
                 234.1 
                 5.4 
                 43.4 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 234.5 
                 2.0 
                 222.3 
                 5.8 
                 38.3 
               
               
                 YDE011 10 uM 
                 3rd 
                 249.0 
                 2.0 
                 234.5 
                 4.7 
                 49.9 
               
               
                   
                 Mean 
                 244.0 
                 2.0 
                 230.3 
                 5.3 
                 43.9 
               
               
                   
                 STDER 
                 4.8 
                 0.0 
                 4.0 
                 0.3 
                 3.4 
               
               
                 PBMC +  
                 1st 
                 379.5 
                 2.0 
                 342.2 
                 6.1 
                 56.1 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 351.5 
                 2.0 
                 319.4 
                 6.1 
                 52.4 
               
               
                 YDE011  
                 3rd 
                 338.5 
                 2.0 
                 308.7 
                 6.0 
                 51.5 
               
               
                 100 uM 
                 Mean 
                 356.5 
                 2.0 
                 323.4 
                 6.1 
                 53.3 
               
               
                   
                 STDER 
                 12.1 
                 0.0 
                 9.9 
                 0.0 
                 1.4 
               
               
                 PBMC +  
                 1st 
                 252.5 
                 2.0 
                 237.5 
                 4.4 
                 54.0 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 225.0 
                 2.0 
                 214.3 
                 6.0 
                 35.7 
               
               
                 YDE043  
                 3rd 
                 225.5 
                 2.0 
                 214.7 
                 4.6 
                 46.7 
               
               
                 0.001 uM 
                 Mean 
                 234.3 
                 2.0 
                 222.1 
                 5.0 
                 45.5 
               
               
                   
                 STDER 
                 9.1 
                 0.0 
                 7.7 
                 0.5 
                 5.3 
               
               
                 PBMC +  
                 1st 
                 294.0 
                 2.0 
                 272.1 
                 4.5 
                 60.5 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 250.5 
                 2.0 
                 235.8 
                 6.6 
                 35.7 
               
               
                 YDE043  
                 3rd 
                 284.5 
                 2.0 
                 264.2 
                 5.0 
                 52.8 
               
               
                 0.01 uM 
                 Mean 
                 276.3 
                 2.0 
                 257.4 
                 5.4 
                 49.7 
               
               
                   
                 STDER 
                 13.2 
                 0.0 
                 11.0 
                 0.6 
                 7.3 
               
               
                 PBMC +  
                 1st 
                 291.0 
                 2.0 
                 269.6 
                 5.0 
                 53.9 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 287.5 
                 2.0 
                 266.7 
                 5.6 
                 47.6 
               
               
                 YDE043 0.1 uM 
                 3rd 
                 294.0 
                 2.0 
                 272.1 
                 5.6 
                 48.6 
               
               
                   
                 Mean 
                 290.8 
                 2.0 
                 269.5 
                 5.4 
                 50.0 
               
               
                   
                 STDER 
                 1.9 
                 0.0 
                 1.6 
                 0.2 
                 2.0 
               
               
                 PBMC +  
                 1st 
                 288.0 
                 2.0 
                 267.1 
                 5.8 
                 46.1 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 269.5 
                 2.0 
                 251.7 
                 4.8 
                 52.4 
               
               
                 YDE043 1 uM 
                 3rd 
                 272.5 
                 2.0 
                 254.2 
                 5.3 
                 48.0 
               
               
                   
                 Mean 
                 276.7 
                 2.0 
                 257.7 
                 5.3 
                 48.8 
               
               
                   
                 STDER 
                 5.7 
                 0.0 
                 4.8 
                 0.3 
                 1.9 
               
               
                 PBMC +  
                 1st 
                 279.5 
                 2.0 
                 260.1 
                 5.2 
                 50.0 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 280.5 
                 2.0 
                 260.9 
                 5.4 
                 48.3 
               
               
                 YDE043 10 uM 
                 3rd 
                 306.5 
                 2.0 
                 282.4 
                 5.6 
                 50.4 
               
               
                   
                 Mean 
                 288.8 
                 2.0 
                 267.8 
                 5.4 
                 49.6 
               
               
                   
                 STDER 
                 8.8 
                 0.0 
                 7.3 
                 0.1 
                 0.6 
               
               
                 PBMC +  
                 1st 
                 428.5 
                 2.0 
                 381.7 
                 6.2 
                 61.6 
               
               
                 LPS(5 ug/ml) +  
                 2nd 
                 370.5 
                 2.0 
                 334.8 
                 6.4 
                 52.3 
               
               
                 YDE043  
                 Mean 
                 399.5 
                 2.0 
                 358.3 
                 6.3 
                 56.9 
               
               
                 100 uM 
                 STDER 
                 29.0 
                 0.0 
                 23.4 
                 0.1 
                 4.6 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 38 
               
             
            
               
                   
               
               
                 Bead name: IL-1 alpha 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.5 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 −0.5 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 0.0 
                 2.0 
                 3.3 
                 7.5 
                 0.4 
               
               
                   
                 2nd 
                 −1.0 
                 2.0 
                 1.0 
                 7.4 
                 0.1 
               
               
                   
                 3rd 
                 −1.0 
                 2.0 
                 1.0 
                 6.6 
                 0.1 
               
               
                   
                 Mean 
                 −0.7 
                 2.0 
                 1.8 
                 7.2 
                 0.2 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.8 
                 0.3 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 516.5 
                 2.0 
                 435.0 
                 6.1 
                 71.3 
               
               
                   
                 2nd 
                 483.0 
                 2.0 
                 410.3 
                 5.8 
                 70.7 
               
               
                   
                 3rd 
                 466.5 
                 2.0 
                 398.1 
                 5.5 
                 72.4 
               
               
                   
                 Mean 
                 488.7 
                 2.0 
                 414.5 
                 5.8 
                 71.5 
               
               
                   
                 STDER 
                 14.7 
                 0.0 
                 10.9 
                 0.2 
                 0.5 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 −1.0 
                 2.0 
                 1.0 
                 5.2 
                 0.2 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                 5.3 
                 4.8 
                 1.1 
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 3.1 
                 5.0 
                 0.6 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 2.2 
                 0.2 
                 0.5 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 −1.0 
                 2.0 
                 1.0 
                 4.4 
                 0.2 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                 5.3 
                 5.8 
                 0.9 
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 3.1 
                 5.1 
                 0.6 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 2.2 
                 0.7 
                 0.3 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 1.0 
                 2.0 
                 5.3 
                 5.5 
                 1.0 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                 5.3 
                 5.5 
                 1.0 
               
               
                   
                 Mean 
                 1.0 
                 2.0 
                 5.3 
                 5.5 
                 1.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.0 
                 0.0 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 20.0 
                 2.0 
                 31.7 
                 6.4 
                 5.0 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                 7.1 
                 6.1 
                 1.2 
               
               
                   
                 Mean 
                 11.0 
                 2.0 
                 19.4 
                 6.3 
                 3.1 
               
               
                   
                 STDER 
                 9.0 
                 0.0 
                 12.3 
                 0.2 
                 1.9 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 4.0 
                 2.0 
                 10.4 
                 6.4 
                 1.6 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                 5.3 
                 7.0 
                 0.8 
               
               
                   
                 Mean 
                 2.5 
                 2.0 
                 7.9 
                 6.7 
                 1.2 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 2.5 
                 0.3 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 599.0 
                 2.0 
                 495.8 
                 6.2 
                 80.0 
               
               
                   
                 2nd 
                 545.0 
                 2.0 
                 456.0 
                 7.6 
                 60.0 
               
               
                   
                 Mean 
                 572.0 
                 2.0 
                 475.9 
                 6.9 
                 70.0 
               
               
                   
                 STDER 
                 27.0 
                 0.0 
                 19.9 
                 0.7 
                 10.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 651.0 
                 2.0 
                 534.1 
                 5.2 
                 102.7 
               
               
                   
                 2nd 
                 539.0 
                 2.0 
                 451.6 
                 5.8 
                 77.9 
               
               
                   
                 Mean 
                 595.0 
                 2.0 
                 492.8 
                 5.5 
                 90.3 
               
               
                   
                 STDER 
                 56.0 
                 0.0 
                 41.3 
                 0.3 
                 12.4 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 471.0 
                 2.0 
                 401.4 
                 6.6 
                 60.8 
               
               
                   
                 2nd 
                 473.5 
                 2.0 
                 403.3 
                 5.7 
                 70.8 
               
               
                   
                 Mean 
                 472.3 
                 2.0 
                 402.4 
                 6.2 
                 65.8 
               
               
                   
                 STDER 
                 1.3 
                 0.0 
                 0.9 
                 0.5 
                 5.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 619.0 
                 2.0 
                 510.5 
                 4.8 
                 106.4 
               
               
                   
                 2nd 
                 421.0 
                 2.0 
                 364.4 
                 4.4 
                 82.8 
               
               
                   
                 Mean 
                 520.0 
                 2.0 
                 437.5 
                 4.6 
                 94.6 
               
               
                   
                 STDER 
                 99.0 
                 0.0 
                 73.0 
                 0.2 
                 11.8 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 493.5 
                 2.0 
                 418.0 
                 5.8 
                 72.1 
               
               
                   
                 2nd 
                 528.0 
                 2.0 
                 443.5 
                 6.4 
                 69.3 
               
               
                   
                 Mean 
                 510.8 
                 2.0 
                 430.8 
                 6.1 
                 70.7 
               
               
                   
                 STDER 
                 17.3 
                 0.0 
                 12.7 
                 0.3 
                 1.4 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 378.0 
                 2.0 
                 332.4 
                 7.1 
                 46.8 
               
               
                   
                 2nd 
                 397.5 
                 2.0 
                 346.9 
                 5.8 
                 59.8 
               
               
                   
                 Mean 
                 387.8 
                 2.0 
                 339.7 
                 6.5 
                 53.3 
               
               
                   
                 STDER 
                 9.8 
                 0.0 
                 7.3 
                 0.7 
                 6.5 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 504.5 
                 2.0 
                 426.2 
                 5.7 
                 74.8 
               
               
                   
                 2nd 
                 553.0 
                 2.0 
                 461.9 
                 7.1 
                 65.1 
               
               
                   
                 3rd 
                 496.0 
                 2.0 
                 419.9 
                 6.6 
                 63.6 
               
               
                   
                 Mean 
                 517.8 
                 2.0 
                 436.0 
                 6.5 
                 67.8 
               
               
                   
                 STDER 
                 17.8 
                 0.0 
                 13.1 
                 0.4 
                 3.5 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 613.0 
                 2.0 
                 506.1 
                 6.4 
                 79.1 
               
               
                   
                 2nd 
                 572.0 
                 2.0 
                 475.9 
                 6.0 
                 79.3 
               
               
                   
                 3rd 
                 584.0 
                 2.0 
                 484.7 
                 6.1 
                 79.5 
               
               
                   
                 Mean 
                 589.7 
                 2.0 
                 488.9 
                 6.2 
                 79.3 
               
               
                   
                 STDER 
                 12.2 
                 0.0 
                 9.0 
                 0.1 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 593.0 
                 2.0 
                 491.3 
                 6.2 
                 79.2 
               
               
                   
                 2nd 
                 580.0 
                 2.0 
                 481.8 
                 5.2 
                 92.6 
               
               
                   
                 3rd 
                 581.0 
                 2.0 
                 482.5 
                 6.2 
                 77.8 
               
               
                   
                 Mean 
                 584.7 
                 2.0 
                 485.2 
                 5.9 
                 83.2 
               
               
                   
                 STDER 
                 4.2 
                 0.0 
                 3.1 
                 0.3 
                 4.7 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 538.5 
                 2.0 
                 451.2 
                 6.3 
                 71.6 
               
               
                   
                 2nd 
                 506.0 
                 2.0 
                 427.3 
                 7.1 
                 60.2 
               
               
                   
                 3rd 
                 515.5 
                 2.0 
                 434.3 
                 6.2 
                 70.0 
               
               
                   
                 Mean 
                 520.0 
                 2.0 
                 437.6 
                 6.5 
                 67.3 
               
               
                   
                 STDER 
                 9.6 
                 0.0 
                 7.1 
                 0.3 
                 3.6 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 568.5 
                 2.0 
                 473.3 
                 6.0 
                 78.9 
               
               
                   
                 2nd 
                 565.0 
                 2.0 
                 470.7 
                 6.4 
                 73.6 
               
               
                   
                 3rd 
                 582.5 
                 2.0 
                 483.6 
                 7.6 
                 63.6 
               
               
                   
                 Mean 
                 572.0 
                 2.0 
                 475.9 
                 6.7 
                 72.0 
               
               
                   
                 STDER 
                 5.3 
                 0.0 
                 3.9 
                 0.5 
                 4.5 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 816.0 
                 2.0 
                 656.9 
                 6.3 
                 104.3 
               
               
                   
                 2nd 
                 762.0 
                 2.0 
                 616.5 
                 6.2 
                 99.4 
               
               
                   
                 3rd 
                 759.0 
                 2.0 
                 614.2 
                 5.0 
                 122.8 
               
               
                   
                 Mean 
                 779.0 
                 2.0 
                 629.2 
                 5.8 
                 108.8 
               
               
                   
                 STDER 
                 18.5 
                 0.0 
                 13.9 
                 0.4 
                 7.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 383.0 
                 2.0 
                 336.1 
                 4.5 
                 74.7 
               
               
                   
                 2nd 
                 382.0 
                 2.0 
                 335.4 
                 5.8 
                 57.8 
               
               
                   
                 3rd 
                 416.5 
                 2.0 
                 361.1 
                 5.8 
                 62.3 
               
               
                   
                 Mean 
                 393.8 
                 2.0 
                 344.2 
                 5.4 
                 64.9 
               
               
                   
                 STDER 
                 11.3 
                 0.0 
                 8.4 
                 0.4 
                 5.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 392.0 
                 2.0 
                 342.8 
                 6.3 
                 54.4 
               
               
                   
                 2nd 
                 414.0 
                 2.0 
                 359.2 
                 6.2 
                 57.9 
               
               
                   
                 3rd 
                 444.5 
                 2.0 
                 381.8 
                 5.1 
                 74.9 
               
               
                   
                 Mean 
                 416.8 
                 2.0 
                 361.3 
                 5.9 
                 62.4 
               
               
                   
                 STDER 
                 15.2 
                 0.0 
                 11.3 
                 0.4 
                 6.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 378.0 
                 2.0 
                 332.4 
                 6.6 
                 50.4 
               
               
                   
                 2nd 
                 352.0 
                 2.0 
                 312.9 
                 5.0 
                 62.6 
               
               
                   
                 3rd 
                 398.0 
                 2.0 
                 347.3 
                 5.7 
                 60.9 
               
               
                   
                 Mean 
                 376.0 
                 2.0 
                 330.9 
                 5.8 
                 58.0 
               
               
                   
                 STDER 
                 13.3 
                 0.0 
                 10.0 
                 0.5 
                 3.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 400.5 
                 2.0 
                 349.2 
                 5.5 
                 63.5 
               
               
                   
                 2nd 
                 363.0 
                 2.0 
                 321.1 
                 4.8 
                 66.9 
               
               
                   
                 3rd 
                 396.0 
                 2.0 
                 345.8 
                 4.8 
                 72.0 
               
               
                   
                 Mean 
                 386.5 
                 2.0 
                 338.7 
                 5.0 
                 67.5 
               
               
                   
                 STDER 
                 11.8 
                 0.0 
                 8.8 
                 0.2 
                 2.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 410.0 
                 2.0 
                 356.2 
                 5.4 
                 66.0 
               
               
                   
                 2nd 
                 372.0 
                 2.0 
                 327.9 
                 5.8 
                 56.5 
               
               
                   
                 3rd 
                 396.0 
                 2.0 
                 345.8 
                 4.7 
                 73.6 
               
               
                   
                 Mean 
                 392.7 
                 2.0 
                 343.3 
                 5.3 
                 65.4 
               
               
                   
                 STDER 
                 11.1 
                 0.0 
                 8.3 
                 0.3 
                 4.9 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 532.0 
                 2.0 
                 446.4 
                 6.1 
                 73.2 
               
               
                   
                 2nd 
                 472.0 
                 2.0 
                 402.2 
                 6.1 
                 65.9 
               
               
                   
                 3rd 
                 518.0 
                 2.0 
                 436.1 
                 6.0 
                 72.7 
               
               
                   
                 Mean 
                 507.3 
                 2.0 
                 428.2 
                 6.1 
                 70.6 
               
               
                   
                 STDER 
                 18.1 
                 0.0 
                 13.4 
                 0.0 
                 2.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 387.0 
                 2.0 
                 339.1 
                 4.4 
                 77.1 
               
               
                   
                 2nd 
                 2522.0 
                 2.0 
                 369.6 
                 6.0 
                 61.6 
               
               
                   
                 3rd 
                 412.0 
                 2.0 
                 357.7 
                 4.6 
                 77.8 
               
               
                   
                 Mean 
                 1107.0 
                 2.0 
                 355.5 
                 5.0 
                 72.1 
               
               
                   
                 STDER 
                 707.5 
                 0.0 
                 8.9 
                 0.5 
                 5.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 405.0 
                 2.0 
                 352.5 
                 4.5 
                 78.3 
               
               
                   
                 2nd 
                 391.5 
                 2.0 
                 342.5 
                 6.6 
                 51.9 
               
               
                   
                 3rd 
                 429.0 
                 2.0 
                 370.4 
                 5.0 
                 74.1 
               
               
                   
                 Mean 
                 408.5 
                 2.0 
                 355.1 
                 5.4 
                 68.1 
               
               
                   
                 STDER 
                 11.0 
                 0.0 
                 8.2 
                 0.6 
                 8.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 417.0 
                 2.0 
                 361.4 
                 5.0 
                 72.3 
               
               
                   
                 2nd 
                 373.5 
                 2.0 
                 329.0 
                 5.6 
                 58.8 
               
               
                   
                 3rd 
                 445.5 
                 2.0 
                 382.6 
                 5.6 
                 68.3 
               
               
                   
                 Mean 
                 412.0 
                 2.0 
                 357.7 
                 5.4 
                 66.5 
               
               
                   
                 STDER 
                 20.9 
                 0.0 
                 15.6 
                 0.2 
                 4.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 394.5 
                 2.0 
                 344.7 
                 5.8 
                 59.4 
               
               
                   
                 2nd 
                 378.0 
                 2.0 
                 332.4 
                 4.8 
                 69.2 
               
               
                   
                 3rd 
                 386.5 
                 2.0 
                 338.7 
                 5.3 
                 63.9 
               
               
                   
                 Mean 
                 386.3 
                 2.0 
                 338.6 
                 5.3 
                 64.2 
               
               
                   
                 STDER 
                 4.8 
                 0.0 
                 3.6 
                 0.3 
                 2.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 411.5 
                 2.0 
                 357.4 
                 5.2 
                 68.7 
               
               
                   
                 2nd 
                 391.0 
                 2.0 
                 342.1 
                 5.4 
                 63.3 
               
               
                   
                 3rd 
                 403.0 
                 2.0 
                 351.0 
                 5.6 
                 62.7 
               
               
                   
                 Mean 
                 401.8 
                 2.0 
                 350.2 
                 5.4 
                 64.9 
               
               
                   
                 STDER 
                 5.9 
                 0.0 
                 4.4 
                 0.1 
                 1.9 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 543.0 
                 2.0 
                 454.5 
                 6.2 
                 73.3 
               
               
                   
                 2nd 
                 488.5 
                 2.0 
                 414.4 
                 6.4 
                 64.7 
               
               
                   
                 Mean 
                 515.8 
                 2.0 
                 434.4 
                 6.3 
                 69.0 
               
               
                   
                 STDER 
                 27.3 
                 0.0 
                 20.1 
                 0.1 
                 4.3 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 39 
               
             
            
               
                   
               
               
                 Bead name: IL-1 beta/IL-1F2 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.5 
                 10.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 −0.5 
                 10.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 10.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 1.5 
                 10.0 
                   
                 7.5 
                 0.0 
               
               
                   
                 2nd 
                 1.5 
                 10.0 
                   
                 7.4 
                 0.0 
               
               
                   
                 3rd 
                 1.5 
                 10.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 Mean 
                 1.5 
                 10.0 
                 #DIV/0! 
                 7.2 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 1906.0 
                 10.0 
                 7351.0 
                 6.1 
                 1205.1 
               
               
                   
                 2nd 
                 1803.0 
                 10.0 
                 6969.3 
                 5.8 
                 1201.6 
               
               
                   
                 3rd 
                 1657.5 
                 10.0 
                 6432.1 
                 5.5 
                 1169.5 
               
               
                   
                 Mean 
                 1788.8 
                 10.0 
                 6917.5 
                 5.8 
                 1192.1 
               
               
                   
                 STDER 
                 72.1 
                 0.0 
                 266.5 
                 0.2 
                 11.3 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 0.5 
                 10.0 
                   
                 5.2 
                 0.0 
               
               
                   
                 2nd 
                 4.5 
                 10.0 
                 5.5 
                 4.8 
                 1.2 
               
               
                   
                 Mean 
                 2.5 
                 10.0 
                 5.5 
                 5.0 
                 0.6 
               
               
                   
                 STDER 
                 2.0 
                 0.0 
                 #DIV/0! 
                 0.2 
                 0.6 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 1.5 
                 10.0 
                   
                 4.4 
                 0.0 
               
               
                   
                 2nd 
                 1.5 
                 10.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 Mean 
                 1.5 
                 10.0 
                 #DIV/0! 
                 5.1 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.7 
                 0.0 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 3.5 
                 10.0 
                   
                 5.5 
                 0.0 
               
               
                   
                 2nd 
                 0.5 
                 10.0 
                   
                 5.5 
                 0.0 
               
               
                   
                 Mean 
                 2.0 
                 10.0 
                 #DIV/0! 
                 5.5 
                 0.0 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 #DIV/0! 
                 0.0 
                 0.0 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 23.5 
                 10.0 
                 122.9 
                 6.4 
                 19.2 
               
               
                   
                 2nd 
                 2.5 
                 10.0 
                   
                 6.1 
                 0.0 
               
               
                   
                 Mean 
                 13.0 
                 10.0 
                 122.9 
                 6.3 
                 9.6 
               
               
                   
                 STDER 
                 10.5 
                 0.0 
                 #DIV/0! 
                 0.2 
                 9.6 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 53.5 
                 10.0 
                 278.4 
                 6.4 
                 43.5 
               
               
                   
                 2nd 
                 9.5 
                 10.0 
                 40.7 
                 7.0 
                 5.8 
               
               
                   
                 Mean 
                 31.5 
                 10.0 
                 159.6 
                 6.7 
                 24.7 
               
               
                   
                 STDER 
                 22.0 
                 0.0 
                 118.9 
                 0.3 
                 18.8 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 2154.5 
                 10.0 
                 8278.0 
                 6.2 
                 1335.2 
               
               
                   
                 2nd 
                 2155.5 
                 10.0 
                 8281.8 
                 7.6 
                 1089.7 
               
               
                   
                 Mean 
                 2155.0 
                 10.0 
                 8279.9 
                 6.9 
                 1212.4 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 1.9 
                 0.7 
                 122.7 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 2558.5 
                 10.0 
                 9809.5 
                 5.2 
                 1886.4 
               
               
                   
                 2nd 
                 2145.5 
                 10.0 
                 8244.3 
                 5.8 
                 1421.4 
               
               
                   
                 Mean 
                 2352.0 
                 10.0 
                 9026.9 
                 5.5 
                 1653.9 
               
               
                   
                 STDER 
                 206.5 
                 0.0 
                 782.6 
                 0.3 
                 232.5 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 2013.5 
                 10.0 
                 7750.8 
                 6.6 
                 1174.4 
               
               
                   
                 2nd 
                 1975.5 
                 10.0 
                 7609.3 
                 5.7 
                 1335.0 
               
               
                   
                 Mean 
                 1994.5 
                 10.0 
                 7680.1 
                 6.2 
                 1254.7 
               
               
                   
                 STDER 
                 19.0 
                 0.0 
                 70.8 
                 0.5 
                 80.3 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 1857.5 
                 10.0 
                 7171.1 
                 4.8 
                 1494.0 
               
               
                   
                 2nd 
                 1850.0 
                 10.0 
                 7143.3 
                 4.4 
                 1623.5 
               
               
                   
                 Mean 
                 1853.8 
                 10.0 
                 7157.2 
                 4.6 
                 1558.7 
               
               
                   
                 STDER 
                 3.8 
                 0.0 
                 13.9 
                 0.2 
                 64.7 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 1858.0 
                 10.0 
                 7172.9 
                 5.8 
                 1236.7 
               
               
                   
                 2nd 
                 1864.5 
                 10.0 
                 7197.0 
                 6.4 
                 1124.5 
               
               
                   
                 Mean 
                 1861.3 
                 10.0 
                 7185.0 
                 6.1 
                 1180.6 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 12.0 
                 0.3 
                 56.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 1437.5 
                 10.0 
                 5623.1 
                 7.1 
                 792.0 
               
               
                   
                 2nd 
                 1392.5 
                 10.0 
                 5457.9 
                 5.8 
                 941.0 
               
               
                   
                 Mean 
                 1415.0 
                 10.0 
                 5540.5 
                 6.5 
                 866.5 
               
               
                   
                 STDER 
                 22.5 
                 0.0 
                 82.6 
                 0.7 
                 74.5 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 2055.5 
                 10.0 
                 7907.5 
                 5.7 
                 1387.3 
               
               
                   
                 2nd 
                 2063.0 
                 10.0 
                 7935.5 
                 7.1 
                 1117.7 
               
               
                   
                 3rd 
                 1811.5 
                 10.0 
                 7000.7 
                 6.6 
                 1060.7 
               
               
                   
                 Mean 
                 1976.7 
                 10.0 
                 7614.6 
                 6.5 
                 1188.6 
               
               
                   
                 STDER 
                 82.6 
                 0.0 
                 307.0 
                 0.4 
                 100.7 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 2188.5 
                 10.0 
                 8405.7 
                 6.4 
                 1313.4 
               
               
                   
                 2nd 
                 2227.5 
                 10.0 
                 8552.3 
                 6.0 
                 1425.4 
               
               
                   
                 3rd 
                 2137.0 
                 10.0 
                 8212.4 
                 6.1 
                 1346.3 
               
               
                   
                 Mean 
                 2184.3 
                 10.0 
                 8390.1 
                 6.2 
                 1361.7 
               
               
                   
                 STDER 
                 26.2 
                 0.0 
                 98.4 
                 0.1 
                 33.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 2151.5 
                 10.0 
                 8266.8 
                 6.2 
                 1333.4 
               
               
                   
                 2nd 
                 2277.5 
                 10.0 
                 8740.8 
                 5.2 
                 1680.9 
               
               
                   
                 3rd 
                 2220.5 
                 10.0 
                 8526.0 
                 6.2 
                 1375.2 
               
               
                   
                 Mean 
                 2216.5 
                 10.0 
                 8511.2 
                 5.9 
                 1463.1 
               
               
                   
                 STDER 
                 36.4 
                 0.0 
                 137.0 
                 0.3 
                 109.6 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 2138.5 
                 10.0 
                 8218.0 
                 6.3 
                 1304.4 
               
               
                   
                 2nd 
                 2032.5 
                 10.0 
                 7821.7 
                 7.1 
                 1101.6 
               
               
                   
                 3rd 
                 1939.5 
                 10.0 
                 7475.4 
                 6.2 
                 1205.7 
               
               
                   
                 Mean 
                 2036.8 
                 10.0 
                 7838.4 
                 6.5 
                 1203.9 
               
               
                   
                 STDER 
                 57.5 
                 0.0 
                 214.5 
                 0.3 
                 58.6 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 2218.5 
                 10.0 
                 8518.5 
                 6.0 
                 1419.7 
               
               
                   
                 2nd 
                 2297.5 
                 10.0 
                 8816.3 
                 6.4 
                 1377.5 
               
               
                   
                 3rd 
                 2251.5 
                 10.0 
                 8642.7 
                 7.6 
                 1137.2 
               
               
                   
                 Mean 
                 2255.8 
                 10.0 
                 8659.2 
                 6.7 
                 1311.5 
               
               
                   
                 STDER 
                 22.9 
                 0.0 
                 86.4 
                 0.5 
                 88.0 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 3353.5 
                 10.0 
                 12944.3 
                 6.3 
                 2054.7 
               
               
                   
                 2nd 
                 3064.5 
                 10.0 
                 11783.1 
                 6.2 
                 1900.5 
               
               
                   
                 3rd 
                 3071.5 
                 10.0 
                 11811.0 
                 5.0 
                 2362.2 
               
               
                   
                 Mean 
                 3163.2 
                 10.0 
                 12179.5 
                 5.8 
                 2105.8 
               
               
                   
                 STDER 
                 95.2 
                 0.0 
                 382.5 
                 0.4 
                 135.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 1675.5 
                 10.0 
                 6498.5 
                 4.5 
                 1444.1 
               
               
                   
                 2nd 
                 1688.0 
                 10.0 
                 6544.6 
                 5.8 
                 1128.4 
               
               
                   
                 3rd 
                 1649.5 
                 10.0 
                 6402.7 
                 5.8 
                 1103.9 
               
               
                   
                 Mean 
                 1671.0 
                 10.0 
                 6481.9 
                 5.4 
                 1225.5 
               
               
                   
                 STDER 
                 11.3 
                 0.0 
                 41.8 
                 0.4 
                 109.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 1721.5 
                 10.0 
                 6668.1 
                 6.3 
                 1058.4 
               
               
                   
                 2nd 
                 1691.5 
                 10.0 
                 6557.5 
                 6.2 
                 1057.7 
               
               
                   
                 3rd 
                 1669.5 
                 10.0 
                 6476.4 
                 5.1 
                 1269.9 
               
               
                   
                 Mean 
                 1694.2 
                 10.0 
                 6567.3 
                 5.9 
                 1128.7 
               
               
                   
                 STDER 
                 15.1 
                 0.0 
                 55.6 
                 0.4 
                 70.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 1623.0 
                 10.0 
                 6305.1 
                 6.6 
                 955.3 
               
               
                   
                 2nd 
                 1642.5 
                 10.0 
                 6376.9 
                 5.0 
                 1275.4 
               
               
                   
                 3rd 
                 1636.5 
                 10.0 
                 6354.8 
                 5.7 
                 1114.9 
               
               
                   
                 Mean 
                 1634.0 
                 10.0 
                 6345.6 
                 5.8 
                 1115.2 
               
               
                   
                 STDER 
                 5.8 
                 0.0 
                 21.2 
                 0.5 
                 92.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 1690.5 
                 10.0 
                 6553.8 
                 5.5 
                 1191.6 
               
               
                   
                 2nd 
                 1663.5 
                 10.0 
                 6454.2 
                 4.8 
                 1344.6 
               
               
                   
                 3rd 
                 1744.5 
                 10.0 
                 6753.1 
                 4.8 
                 1406.9 
               
               
                   
                 Mean 
                 1699.5 
                 10.0 
                 6587.0 
                 5.0 
                 1314.4 
               
               
                   
                 STDER 
                 23.8 
                 0.0 
                 87.8 
                 0.2 
                 64.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 1814.0 
                 10.0 
                 7010.0 
                 5.4 
                 1298.1 
               
               
                   
                 2nd 
                 1752.5 
                 10.0 
                 6782.6 
                 5.8 
                 1169.4 
               
               
                   
                 3rd 
                 1813.0 
                 10.0 
                 7006.3 
                 4.7 
                 1490.7 
               
               
                   
                 Mean 
                 1793.2 
                 10.0 
                 6933.0 
                 5.3 
                 1319.4 
               
               
                   
                 STDER 
                 20.3 
                 0.0 
                 75.2 
                 0.3 
                 93.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 2213.0 
                 10.0 
                 8497.8 
                 6.1 
                 1393.1 
               
               
                   
                 2nd 
                 2324.5 
                 10.0 
                 8918.4 
                 6.1 
                 1462.0 
               
               
                   
                 3rd 
                 2242.5 
                 10.0 
                 8608.8 
                 6.0 
                 1434.8 
               
               
                   
                 Mean 
                 2260.0 
                 10.0 
                 8675.0 
                 6.1 
                 1430.0 
               
               
                   
                 STDER 
                 33.4 
                 0.0 
                 125.8 
                 0.0 
                 20.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 1640.5 
                 10.0 
                 6369.5 
                 4.4 
                 1447.6 
               
               
                   
                 2nd 
                 1821.5 
                 10.0 
                 7037.7 
                 6.0 
                 1173.0 
               
               
                   
                 3rd 
                 1673.5 
                 10.0 
                 6491.1 
                 4.6 
                 1411.1 
               
               
                   
                 Mean 
                 1711.8 
                 10.0 
                 6632.8 
                 5.0 
                 1343.9 
               
               
                   
                 STDER 
                 55.7 
                 0.0 
                 205.5 
                 0.5 
                 86.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 1657.5 
                 10.0 
                 6432.1 
                 4.5 
                 1429.4 
               
               
                   
                 2nd 
                 1718.0 
                 10.0 
                 6655.2 
                 6.6 
                 1008.4 
               
               
                   
                 3rd 
                 1708.5 
                 10.0 
                 6620.2 
                 5.0 
                 1324.0 
               
               
                   
                 Mean 
                 1694.7 
                 10.0 
                 6569.2 
                 5.4 
                 1253.9 
               
               
                   
                 STDER 
                 18.8 
                 0.0 
                 69.3 
                 0.6 
                 126.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 1553.5 
                 10.0 
                 6049.3 
                 5.0 
                 1209.9 
               
               
                   
                 2nd 
                 1644.5 
                 10.0 
                 6384.2 
                 5.6 
                 1140.0 
               
               
                   
                 3rd 
                 1686.5 
                 10.0 
                 6539.0 
                 5.6 
                 1167.7 
               
               
                   
                 Mean 
                 1628.2 
                 10.0 
                 6324.2 
                 5.4 
                 1172.5 
               
               
                   
                 STDER 
                 39.3 
                 0.0 
                 144.5 
                 0.2 
                 20.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 1622.5 
                 10.0 
                 6303.2 
                 5.8 
                 1086.8 
               
               
                   
                 2nd 
                 1524.5 
                 10.0 
                 5942.7 
                 4.8 
                 1238.1 
               
               
                   
                 3rd 
                 1632.5 
                 10.0 
                 6340.0 
                 5.3 
                 1196.2 
               
               
                   
                 Mean 
                 1593.2 
                 10.0 
                 6195.3 
                 5.3 
                 1173.7 
               
               
                   
                 STDER 
                 34.5 
                 0.0 
                 126.8 
                 0.3 
                 45.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 1644.0 
                 10.0 
                 6382.4 
                 5.2 
                 1227.4 
               
               
                   
                 2nd 
                 1712.5 
                 10.0 
                 6634.9 
                 5.4 
                 1228.7 
               
               
                   
                 3rd 
                 1717.5 
                 10.0 
                 6653.4 
                 5.6 
                 1188.1 
               
               
                   
                 Mean 
                 1691.3 
                 10.0 
                 6556.9 
                 5.4 
                 1214.7 
               
               
                   
                 STDER 
                 23.7 
                 0.0 
                 87.4 
                 0.1 
                 13.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 2278.5 
                 10.0 
                 8744.5 
                 6.2 
                 1410.4 
               
               
                   
                 2nd 
                 1993.0 
                 10.0 
                 7674.4 
                 6.4 
                 1199.1 
               
               
                   
                 Mean 
                 2135.8 
                 10.0 
                 8209.5 
                 6.3 
                 1304.8 
               
               
                   
                 STDER 
                 142.8 
                 0.0 
                 535.0 
                 0.1 
                 105.6 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 40 
               
             
            
               
                   
               
               
                 Bead name: TIMP-1 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 conc/live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.0 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 2918.0 
                 2.0 
                 4364.2 
                 7.5 
                 581.9 
               
               
                   
                 2nd 
                 3072.0 
                 2.0 
                 4558.5 
                 7.4 
                 616.0 
               
               
                   
                 3rd 
                 2936.5 
                 2.0 
                 4387.5 
                 6.6 
                 664.8 
               
               
                   
                 Mean 
                 2975.5 
                 2.0 
                 4436.7 
                 7.2 
                 620.9 
               
               
                   
                 STDER 
                 48.5 
                 0.0 
                 61.3 
                 0.3 
                 24.1 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 2018.0 
                 2.0 
                 3219.0 
                 6.1 
                 527.7 
               
               
                   
                 2nd 
                 2060.5 
                 2.0 
                 3273.7 
                 5.8 
                 564.4 
               
               
                   
                 3rd 
                 2159.5 
                 2.0 
                 3400.7 
                 5.5 
                 618.3 
               
               
                   
                 Mean 
                 2079.3 
                 2.0 
                 3297.8 
                 5.8 
                 570.1 
               
               
                   
                 STDER 
                 41.9 
                 0.0 
                 53.8 
                 0.2 
                 26.3 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 3456.0 
                 2.0 
                 5042.4 
                 5.2 
                 969.7 
               
               
                   
                 2nd 
                 3876.0 
                 2.0 
                 5571.4 
                 4.8 
                 1160.7 
               
               
                   
                 Mean 
                 3666.0 
                 2.0 
                 5306.9 
                 5.0 
                 1065.2 
               
               
                   
                 STDER 
                 210.0 
                 0.0 
                 264.5 
                 0.2 
                 95.5 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 3084.0 
                 2.0 
                 4573.6 
                 4.4 
                 1039.5 
               
               
                   
                 2nd 
                 3614.5 
                 2.0 
                 5242.0 
                 5.8 
                 903.8 
               
               
                   
                 Mean 
                 3349.3 
                 2.0 
                 4907.8 
                 5.1 
                 971.6 
               
               
                   
                 STDER 
                 265.3 
                 0.0 
                 334.2 
                 0.7 
                 67.8 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 3546.0 
                 2.0 
                 5155.8 
                 5.5 
                 937.4 
               
               
                   
                 2nd 
                 3491.5 
                 2.0 
                 5087.1 
                 5.5 
                 924.9 
               
               
                   
                 Mean 
                 3518.8 
                 2.0 
                 5121.4 
                 5.5 
                 931.2 
               
               
                   
                 STDER 
                 27.3 
                 0.0 
                 34.3 
                 0.0 
                 6.2 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 5828.5 
                 2.0 
                 8049.3 
                 6.4 
                 1257.7 
               
               
                   
                 2nd 
                 3505.0 
                 2.0 
                 5104.1 
                 6.1 
                 836.7 
               
               
                   
                 Mean 
                 4666.8 
                 2.0 
                 6576.7 
                 6.3 
                 1047.2 
               
               
                   
                 STDER 
                 1161.8 
                 0.0 
                 1472.6 
                 0.2 
                 210.5 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 4917.0 
                 2.0 
                 6886.9 
                 6.4 
                 1076.1 
               
               
                   
                 2nd 
                 4391.5 
                 2.0 
                 6221.7 
                 7.0 
                 888.8 
               
               
                   
                 Mean 
                 4654.3 
                 2.0 
                 6554.3 
                 6.7 
                 982.4 
               
               
                   
                 STDER 
                 262.8 
                 0.0 
                 332.6 
                 0.3 
                 93.6 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 1751.5 
                 2.0 
                 2874.2 
                 6.2 
                 463.6 
               
               
                   
                 2nd 
                 1703.0 
                 2.0 
                 2810.9 
                 7.6 
                 369.9 
               
               
                   
                 Mean 
                 1727.3 
                 2.0 
                 2842.6 
                 6.9 
                 416.7 
               
               
                   
                 STDER 
                 24.3 
                 0.0 
                 31.6 
                 0.7 
                 46.9 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 1905.5 
                 2.0 
                 3073.9 
                 5.2 
                 591.1 
               
               
                   
                 2nd 
                 1832.5 
                 2.0 
                 2979.4 
                 5.8 
                 513.7 
               
               
                   
                 Mean 
                 1869.0 
                 2.0 
                 3026.7 
                 5.5 
                 552.4 
               
               
                   
                 STDER 
                 36.5 
                 0.0 
                 47.3 
                 0.3 
                 38.7 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 1721.0 
                 2.0 
                 2834.4 
                 6.6 
                 429.5 
               
               
                   
                 2nd 
                 1669.0 
                 2.0 
                 2766.5 
                 5.7 
                 485.4 
               
               
                   
                 Mean 
                 1695.0 
                 2.0 
                 2800.5 
                 6.2 
                 457.4 
               
               
                   
                 STDER 
                 26.0 
                 0.0 
                 34.0 
                 0.5 
                 27.9 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 1780.5 
                 2.0 
                 2911.9 
                 4.8 
                 606.6 
               
               
                   
                 2nd 
                 1783.0 
                 2.0 
                 2915.2 
                 4.4 
                 662.5 
               
               
                   
                 Mean 
                 1781.8 
                 2.0 
                 2913.5 
                 4.6 
                 634.6 
               
               
                   
                 STDER 
                 1.3 
                 0.0 
                 1.6 
                 0.2 
                 27.9 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 1805.0 
                 2.0 
                 2943.7 
                 5.8 
                 507.5 
               
               
                   
                 2nd 
                 1994.0 
                 2.0 
                 3188.1 
                 6.4 
                 498.1 
               
               
                   
                 Mean 
                 1899.5 
                 2.0 
                 3065.9 
                 6.1 
                 502.8 
               
               
                   
                 STDER 
                 94.5 
                 0.0 
                 122.2 
                 0.3 
                 4.7 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 2052.5 
                 2.0 
                 3263.4 
                 7.1 
                 459.6 
               
               
                   
                 2nd 
                 1981.5 
                 2.0 
                 3172.0 
                 5.8 
                 546.9 
               
               
                   
                 Mean 
                 2017.0 
                 2.0 
                 3217.7 
                 6.5 
                 503.3 
               
               
                   
                 STDER 
                 35.5 
                 0.0 
                 45.7 
                 0.7 
                 43.6 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 1596.0 
                 2.0 
                 2670.8 
                 5.7 
                 468.6 
               
               
                   
                 2nd 
                 1464.5 
                 2.0 
                 2497.3 
                 7.1 
                 351.7 
               
               
                   
                 3rd 
                 1648.5 
                 2.0 
                 2739.7 
                 6.6 
                 415.1 
               
               
                   
                 Mean 
                 1569.7 
                 2.0 
                 2635.9 
                 6.5 
                 411.8 
               
               
                   
                 STDER 
                 54.7 
                 0.0 
                 72.1 
                 0.4 
                 33.8 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 1569.5 
                 2.0 
                 2636.0 
                 6.4 
                 411.9 
               
               
                   
                 2nd 
                 1506.0 
                 2.0 
                 2552.3 
                 6.0 
                 425.4 
               
               
                   
                 3rd 
                 1668.0 
                 2.0 
                 2765.2 
                 6.1 
                 453.3 
               
               
                   
                 Mean 
                 1581.2 
                 2.0 
                 2651.1 
                 6.2 
                 430.2 
               
               
                   
                 STDER 
                 47.1 
                 0.0 
                 61.9 
                 0.1 
                 12.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 1634.5 
                 2.0 
                 2721.3 
                 6.2 
                 438.9 
               
               
                   
                 2nd 
                 1465.5 
                 2.0 
                 2498.6 
                 5.2 
                 480.5 
               
               
                   
                 3rd 
                 1526.0 
                 2.0 
                 2578.7 
                 6.2 
                 415.9 
               
               
                   
                 Mean 
                 1542.0 
                 2.0 
                 2599.6 
                 5.9 
                 445.1 
               
               
                   
                 STDER 
                 49.4 
                 0.0 
                 65.1 
                 0.3 
                 18.9 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 1553.5 
                 2.0 
                 2614.9 
                 6.3 
                 415.1 
               
               
                   
                 2nd 
                 1585.0 
                 2.0 
                 2656.4 
                 7.1 
                 374.1 
               
               
                   
                 3rd 
                 1654.5 
                 2.0 
                 2747.5 
                 6.2 
                 443.2 
               
               
                   
                 Mean 
                 1597.7 
                 2.0 
                 2672.9 
                 6.5 
                 410.8 
               
               
                   
                 STDER 
                 29.8 
                 0.0 
                 39.2 
                 0.3 
                 20.0 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 1650.0 
                 2.0 
                 2741.7 
                 6.0 
                 456.9 
               
               
                   
                 2nd 
                 1695.5 
                 2.0 
                 2801.1 
                 6.4 
                 437.7 
               
               
                   
                 3rd 
                 1495.5 
                 2.0 
                 2538.4 
                 7.6 
                 334.0 
               
               
                   
                 Mean 
                 1613.7 
                 2.0 
                 2693.7 
                 6.7 
                 409.5 
               
               
                   
                 STDER 
                 60.5 
                 0.0 
                 79.6 
                 0.5 
                 38.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 2179.0 
                 2.0 
                 3425.7 
                 6.3 
                 543.8 
               
               
                   
                 2nd 
                 2135.0 
                 2.0 
                 3369.3 
                 6.2 
                 543.4 
               
               
                   
                 3rd 
                 1987.0 
                 2.0 
                 3179.1 
                 5.0 
                 635.8 
               
               
                   
                 Mean 
                 2100.3 
                 2.0 
                 3324.7 
                 5.8 
                 574.3 
               
               
                   
                 STDER 
                 58.1 
                 0.0 
                 74.6 
                 0.4 
                 30.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 974.0 
                 2.0 
                 1831.6 
                 4.5 
                 407.0 
               
               
                   
                 2nd 
                 852.5 
                 2.0 
                 1659.9 
                 5.8 
                 286.2 
               
               
                   
                 3rd 
                 1013.5 
                 2.0 
                 1886.6 
                 5.8 
                 325.3 
               
               
                   
                 Mean 
                 946.7 
                 2.0 
                 1792.7 
                 5.4 
                 339.5 
               
               
                   
                 STDER 
                 48.4 
                 0.0 
                 68.3 
                 0.4 
                 35.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 936.0 
                 2.0 
                 1778.3 
                 6.3 
                 282.3 
               
               
                   
                 2nd 
                 959.5 
                 2.0 
                 1811.3 
                 6.2 
                 292.1 
               
               
                   
                 3rd 
                 1132.0 
                 2.0 
                 2050.0 
                 5.1 
                 402.0 
               
               
                   
                 Mean 
                 1009.2 
                 2.0 
                 1879.9 
                 5.9 
                 325.5 
               
               
                   
                 STDER 
                 61.8 
                 0.0 
                 85.6 
                 0.4 
                 38.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 1008.5 
                 2.0 
                 1879.7 
                 6.6 
                 284.8 
               
               
                   
                 2nd 
                 865.0 
                 2.0 
                 1677.8 
                 5.0 
                 335.6 
               
               
                   
                 3rd 
                 1049.0 
                 2.0 
                 1935.9 
                 5.7 
                 339.6 
               
               
                   
                 Mean 
                 974.2 
                 2.0 
                 1831.1 
                 5.8 
                 320.0 
               
               
                   
                 STDER 
                 55.8 
                 0.0 
                 78.4 
                 0.5 
                 17.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 1001.5 
                 2.0 
                 1869.9 
                 5.5 
                 340.0 
               
               
                   
                 2nd 
                 916.5 
                 2.0 
                 1750.8 
                 4.8 
                 364.7 
               
               
                   
                 3rd 
                 918.5 
                 2.0 
                 1753.6 
                 4.8 
                 365.3 
               
               
                   
                 Mean 
                 945.5 
                 2.0 
                 1791.4 
                 5.0 
                 356.7 
               
               
                   
                 STDER 
                 28.0 
                 0.0 
                 39.3 
                 0.2 
                 8.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 966.0 
                 2.0 
                 1820.4 
                 5.4 
                 337.1 
               
               
                   
                 2nd 
                 947.5 
                 2.0 
                 1794.4 
                 5.8 
                 309.4 
               
               
                   
                 3rd 
                 873.5 
                 2.0 
                 1689.9 
                 4.7 
                 359.5 
               
               
                   
                 Mean 
                 929.0 
                 2.0 
                 1768.2 
                 5.3 
                 335.3 
               
               
                   
                 STDER 
                 28.3 
                 0.0 
                 39.9 
                 0.3 
                 14.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 1336.5 
                 2.0 
                 2326.8 
                 6.1 
                 381.4 
               
               
                   
                 2nd 
                 1184.5 
                 2.0 
                 2121.7 
                 6.1 
                 347.8 
               
               
                   
                 3rd 
                 1172.0 
                 2.0 
                 2104.6 
                 6.0 
                 350.8 
               
               
                   
                 Mean 
                 1231.0 
                 2.0 
                 2184.4 
                 6.1 
                 360.0 
               
               
                   
                 STDER 
                 52.9 
                 0.0 
                 71.4 
                 0.0 
                 10.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 1014.0 
                 2.0 
                 1887.3 
                 4.4 
                 428.9 
               
               
                   
                 2nd 
                 1129.5 
                 2.0 
                 2046.6 
                 6.0 
                 341.1 
               
               
                   
                 3rd 
                 1147.0 
                 2.0 
                 2070.5 
                 4.6 
                 450.1 
               
               
                   
                 Mean 
                 1096.8 
                 2.0 
                 2001.5 
                 5.0 
                 406.7 
               
               
                   
                 STDER 
                 41.7 
                 0.0 
                 57.5 
                 0.5 
                 33.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 1101.0 
                 2.0 
                 2007.5 
                 4.5 
                 446.1 
               
               
                   
                 2nd 
                 1089.5 
                 2.0 
                 1991.7 
                 6.6 
                 301.8 
               
               
                   
                 3rd 
                 1067.0 
                 2.0 
                 1960.7 
                 5.0 
                 392.1 
               
               
                   
                 Mean 
                 1085.8 
                 2.0 
                 1986.7 
                 5.4 
                 380.0 
               
               
                   
                 STDER 
                 10.0 
                 0.0 
                 13.7 
                 0.6 
                 42.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 987.5 
                 2.0 
                 1850.4 
                 5.0 
                 370.1 
               
               
                   
                 2nd 
                 893.5 
                 2.0 
                 1718.3 
                 5.6 
                 306.8 
               
               
                   
                 3rd 
                 1037.5 
                 2.0 
                 1919.9 
                 5.6 
                 342.8 
               
               
                   
                 Mean 
                 972.8 
                 2.0 
                 1829.5 
                 5.4 
                 339.9 
               
               
                   
                 STDER 
                 42.2 
                 0.0 
                 59.2 
                 0.2 
                 18.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 1043.5 
                 2.0 
                 1928.2 
                 5.8 
                 332.5 
               
               
                   
                 2nd 
                 958.5 
                 2.0 
                 1809.9 
                 4.8 
                 377.1 
               
               
                   
                 3rd 
                 935.0 
                 2.0 
                 1776.9 
                 5.3 
                 335.3 
               
               
                   
                 Mean 
                 979.0 
                 2.0 
                 1838.3 
                 5.3 
                 348.3 
               
               
                   
                 STDER 
                 33.0 
                 0.0 
                 46.0 
                 0.3 
                 14.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 990.0 
                 2.0 
                 1853.9 
                 5.2 
                 356.5 
               
               
                   
                 2nd 
                 1032.0 
                 2.0 
                 1912.3 
                 5.4 
                 354.1 
               
               
                   
                 3rd 
                 929.0 
                 2.0 
                 1768.4 
                 5.6 
                 315.8 
               
               
                   
                 Mean 
                 983.7 
                 2.0 
                 1844.9 
                 5.4 
                 342.1 
               
               
                   
                 STDER 
                 29.9 
                 0.0 
                 41.8 
                 0.1 
                 13.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 1277.5 
                 2.0 
                 2247.5 
                 6.2 
                 362.5 
               
               
                   
                 2nd 
                 1143.0 
                 2.0 
                 2065.1 
                 6.4 
                 322.7 
               
               
                   
                 Mean 
                 1210.3 
                 2.0 
                 2156.3 
                 6.3 
                 342.6 
               
               
                   
                 STDER 
                 67.3 
                 0.0 
                 91.2 
                 0.1 
                 19.9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 41 
               
             
            
               
                   
               
               
                 Bead name: GM-CSF 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                 conc/ 
               
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 −1.5 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 1.5 
                 2.0 
                 NaN 
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 1.5 
                 2.0 
                   
                 7.5 
                 0.0 
               
               
                   
                 2nd 
                 3.5 
                 2.0 
                   
                 7.4 
                 0.0 
               
               
                   
                 3rd 
                 2.5 
                 2.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 Mean 
                 2.5 
                 2.0 
                 #DIV/0! 
                 7.2 
                 0.0 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 142.5 
                 2.0 
                 123.7 
                 6.1 
                 20.3 
               
               
                   
                 2nd 
                 133.5 
                 2.0 
                 116.3 
                 5.8 
                 20.0 
               
               
                   
                 3rd 
                 132.5 
                 2.0 
                 115.4 
                 5.5 
                 21.0 
               
               
                   
                 Mean 
                 136.2 
                 2.0 
                 118.5 
                 5.8 
                 20.4 
               
               
                   
                 STDER 
                 3.2 
                 0.0 
                 2.6 
                 0.2 
                 0.3 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 8.5 
                 2.0 
                 5.3 
                 5.2 
                 1.0 
               
               
                   
                 2nd 
                 9.5 
                 2.0 
                 6.3 
                 4.8 
                 1.3 
               
               
                   
                 Mean 
                 9.0 
                 2.0 
                 5.8 
                 5.0 
                 1.2 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 0.5 
                 0.2 
                 0.2 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 5.5 
                 2.0 
                 1.9 
                 4.4 
                 0.4 
               
               
                   
                 2nd 
                 10.0 
                 2.0 
                 6.9 
                 5.8 
                 1.2 
               
               
                   
                 Mean 
                 7.8 
                 2.0 
                 4.4 
                 5.1 
                 0.8 
               
               
                   
                 STDER 
                 2.3 
                 0.0 
                 2.5 
                 0.7 
                 0.4 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 8.0 
                 2.0 
                 4.7 
                 5.5 
                 0.9 
               
               
                   
                 2nd 
                 7.5 
                 2.0 
                 4.2 
                 5.5 
                 0.8 
               
               
                   
                 Mean 
                 7.8 
                 2.0 
                 4.4 
                 5.5 
                 0.8 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.3 
                 0.0 
                 0.1 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 37.5 
                 2.0 
                 33.5 
                 6.4 
                 5.2 
               
               
                   
                 2nd 
                 16.5 
                 2.0 
                 13.5 
                 6.1 
                 2.2 
               
               
                   
                 Mean 
                 27.0 
                 2.0 
                 23.5 
                 6.3 
                 3.7 
               
               
                   
                 STDER 
                 10.5 
                 0.0 
                 10.0 
                 0.2 
                 1.5 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 17.5 
                 2.0 
                 14.5 
                 6.4 
                 2.3 
               
               
                   
                 2nd 
                 10.5 
                 2.0 
                 7.4 
                 7.0 
                 1.1 
               
               
                   
                 Mean 
                 14.0 
                 2.0 
                 11.0 
                 6.7 
                 1.7 
               
               
                   
                 STDER 
                 3.5 
                 0.0 
                 3.6 
                 0.3 
                 0.6 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 174.5 
                 2.0 
                 149.9 
                 6.2 
                 24.2 
               
               
                   
                 2nd 
                 160.5 
                 2.0 
                 138.5 
                 7.6 
                 18.2 
               
               
                   
                 Mean 
                 167.5 
                 2.0 
                 144.2 
                 6.9 
                 21.2 
               
               
                   
                 STDER 
                 7.0 
                 0.0 
                 5.7 
                 0.7 
                 3.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 193.5 
                 2.0 
                 165.2 
                 5.2 
                 31.8 
               
               
                   
                 2nd 
                 189.5 
                 2.0 
                 162.0 
                 5.8 
                 27.9 
               
               
                   
                 Mean 
                 191.5 
                 2.0 
                 163.6 
                 5.5 
                 29.9 
               
               
                   
                 STDER 
                 2.0 
                 0.0 
                 1.6 
                 0.3 
                 1.9 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 124.5 
                 2.0 
                 108.8 
                 6.6 
                 16.5 
               
               
                   
                 2nd 
                 133.0 
                 2.0 
                 115.9 
                 5.7 
                 20.3 
               
               
                   
                 Mean 
                 128.8 
                 2.0 
                 112.3 
                 6.2 
                 18.4 
               
               
                   
                 STDER 
                 4.3 
                 0.0 
                 3.5 
                 0.5 
                 1.9 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 125.5 
                 2.0 
                 109.6 
                 4.8 
                 22.8 
               
               
                   
                 2nd 
                 109.0 
                 2.0 
                 95.8 
                 4.4 
                 21.8 
               
               
                   
                 Mean 
                 117.3 
                 2.0 
                 102.7 
                 4.6 
                 22.3 
               
               
                   
                 STDER 
                 8.3 
                 0.0 
                 6.9 
                 0.2 
                 0.5 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 97.5 
                 2.0 
                 86.1 
                 5.8 
                 14.9 
               
               
                   
                 2nd 
                 103.5 
                 2.0 
                 91.2 
                 6.4 
                 14.3 
               
               
                   
                 Mean 
                 100.5 
                 2.0 
                 88.7 
                 6.1 
                 14.6 
               
               
                   
                 STDER 
                 3.0 
                 0.0 
                 2.5 
                 0.3 
                 0.3 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 96.5 
                 2.0 
                 85.3 
                 7.1 
                 12.0 
               
               
                   
                 2nd 
                 98.5 
                 2.0 
                 87.0 
                 5.8 
                 15.0 
               
               
                   
                 Mean 
                 97.5 
                 2.0 
                 86.1 
                 6.5 
                 13.5 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 0.8 
                 0.7 
                 1.5 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 161.0 
                 2.0 
                 138.9 
                 5.7 
                 24.4 
               
               
                   
                 2nd 
                 187.5 
                 2.0 
                 160.4 
                 7.1 
                 22.6 
               
               
                   
                 3rd 
                 212.0 
                 2.0 
                 180.1 
                 6.6 
                 27.3 
               
               
                   
                 Mean 
                 186.8 
                 2.0 
                 159.8 
                 6.5 
                 24.7 
               
               
                   
                 STDER 
                 14.7 
                 0.0 
                 11.9 
                 0.4 
                 1.4 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 192.5 
                 2.0 
                 164.4 
                 6.4 
                 25.7 
               
               
                   
                 2nd 
                 186.5 
                 2.0 
                 159.6 
                 6.0 
                 26.6 
               
               
                   
                 3rd 
                 178.5 
                 2.0 
                 153.1 
                 6.1 
                 25.1 
               
               
                   
                 Mean 
                 185.8 
                 2.0 
                 159.0 
                 6.2 
                 25.8 
               
               
                   
                 STDER 
                 4.1 
                 0.0 
                 3.3 
                 0.1 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 175.5 
                 2.0 
                 150.7 
                 6.2 
                 24.3 
               
               
                   
                 2nd 
                 157.0 
                 2.0 
                 135.6 
                 5.2 
                 26.1 
               
               
                   
                 3rd 
                 153.5 
                 2.0 
                 132.7 
                 6.2 
                 21.4 
               
               
                   
                 Mean 
                 162.0 
                 2.0 
                 139.7 
                 5.9 
                 23.9 
               
               
                   
                 STDER 
                 6.8 
                 0.0 
                 5.6 
                 0.3 
                 1.4 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 159.0 
                 2.0 
                 137.2 
                 6.3 
                 21.8 
               
               
                   
                 2nd 
                 154.0 
                 2.0 
                 133.1 
                 7.1 
                 18.8 
               
               
                   
                 3rd 
                 178.5 
                 2.0 
                 153.1 
                 6.2 
                 24.7 
               
               
                   
                 Mean 
                 163.8 
                 2.0 
                 141.2 
                 6.5 
                 21.7 
               
               
                   
                 STDER 
                 7.5 
                 0.0 
                 6.1 
                 0.3 
                 1.7 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 178.5 
                 2.0 
                 153.1 
                 6.0 
                 25.5 
               
               
                   
                 2nd 
                 167.0 
                 2.0 
                 143.8 
                 6.4 
                 22.5 
               
               
                   
                 3rd 
                 171.5 
                 2.0 
                 147.4 
                 7.6 
                 19.4 
               
               
                   
                 Mean 
                 172.3 
                 2.0 
                 148.1 
                 6.7 
                 22.5 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 2.7 
                 0.5 
                 1.8 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 287.0 
                 2.0 
                 239.6 
                 6.3 
                 38.0 
               
               
                   
                 2nd 
                 257.5 
                 2.0 
                 216.3 
                 6.2 
                 34.9 
               
               
                   
                 3rd 
                 257.5 
                 2.0 
                 216.3 
                 5.0 
                 43.3 
               
               
                   
                 Mean 
                 267.3 
                 2.0 
                 224.0 
                 5.8 
                 38.7 
               
               
                   
                 STDER 
                 9.8 
                 0.0 
                 7.8 
                 0.4 
                 2.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 105.0 
                 2.0 
                 92.5 
                 4.5 
                 20.6 
               
               
                   
                 2nd 
                 109.5 
                 2.0 
                 96.3 
                 5.8 
                 16.6 
               
               
                   
                 3rd 
                 116.5 
                 2.0 
                 102.1 
                 5.8 
                 17.6 
               
               
                   
                 Mean 
                 110.3 
                 2.0 
                 97.0 
                 5.4 
                 18.3 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 2.8 
                 0.4 
                 1.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 102.5 
                 2.0 
                 90.4 
                 6.3 
                 14.3 
               
               
                   
                 2nd 
                 123.5 
                 2.0 
                 108.0 
                 6.2 
                 17.4 
               
               
                   
                 3rd 
                 127.5 
                 2.0 
                 111.3 
                 5.1 
                 21.8 
               
               
                   
                 Mean 
                 117.8 
                 2.0 
                 103.2 
                 5.9 
                 17.9 
               
               
                   
                 STDER 
                 7.8 
                 0.0 
                 6.5 
                 0.4 
                 2.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 114.5 
                 2.0 
                 100.5 
                 6.6 
                 15.2 
               
               
                   
                 2nd 
                 103.0 
                 2.0 
                 90.8 
                 5.0 
                 18.2 
               
               
                   
                 3rd 
                 108.5 
                 2.0 
                 95.4 
                 5.7 
                 16.7 
               
               
                   
                 Mean 
                 108.7 
                 2.0 
                 95.6 
                 5.8 
                 16.7 
               
               
                   
                 STDER 
                 3.3 
                 0.0 
                 2.8 
                 0.5 
                 0.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 109.5 
                 2.0 
                 96.3 
                 5.5 
                 17.5 
               
               
                   
                 2nd 
                 105.0 
                 2.0 
                 92.5 
                 4.8 
                 19.3 
               
               
                   
                 3rd 
                 105.5 
                 2.0 
                 92.9 
                 4.8 
                 19.4 
               
               
                   
                 Mean 
                 106.7 
                 2.0 
                 93.9 
                 5.0 
                 18.7 
               
               
                   
                 STDER 
                 1.4 
                 0.0 
                 1.2 
                 0.2 
                 0.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 117.5 
                 2.0 
                 103.0 
                 5.4 
                 19.1 
               
               
                   
                 2nd 
                 105.5 
                 2.0 
                 92.9 
                 5.8 
                 16.0 
               
               
                   
                 3rd 
                 112.5 
                 2.0 
                 98.8 
                 4.7 
                 21.0 
               
               
                   
                 Mean 
                 111.8 
                 2.0 
                 98.2 
                 5.3 
                 18.7 
               
               
                   
                 STDER 
                 3.5 
                 0.0 
                 2.9 
                 0.3 
                 1.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 173.5 
                 2.0 
                 149.0 
                 6.1 
                 24.4 
               
               
                   
                 2nd 
                 147.5 
                 2.0 
                 127.8 
                 6.1 
                 21.0 
               
               
                   
                 3rd 
                 150.5 
                 2.0 
                 130.3 
                 6.0 
                 21.7 
               
               
                   
                 Mean 
                 157.2 
                 2.0 
                 135.7 
                 6.1 
                 22.4 
               
               
                   
                 STDER 
                 8.2 
                 0.0 
                 6.7 
                 0.0 
                 1.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 96.5 
                 2.0 
                 85.3 
                 4.4 
                 19.4 
               
               
                   
                 2nd 
                 118.5 
                 2.0 
                 103.8 
                 6.0 
                 17.3 
               
               
                   
                 3rd 
                 115.5 
                 2.0 
                 101.3 
                 4.6 
                 22.0 
               
               
                   
                 Mean 
                 110.2 
                 2.0 
                 96.8 
                 5.0 
                 19.6 
               
               
                   
                 STDER 
                 6.9 
                 0.0 
                 5.8 
                 0.5 
                 1.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 107.5 
                 2.0 
                 94.6 
                 4.5 
                 21.0 
               
               
                   
                 2nd 
                 97.5 
                 2.0 
                 86.1 
                 6.6 
                 13.1 
               
               
                   
                 3rd 
                 111.5 
                 2.0 
                 97.9 
                 5.0 
                 19.6 
               
               
                   
                 Mean 
                 105.5 
                 2.0 
                 92.9 
                 5.4 
                 17.9 
               
               
                   
                 STDER 
                 4.2 
                 0.0 
                 3.5 
                 0.6 
                 2.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 99.5 
                 2.0 
                 87.8 
                 5.0 
                 17.6 
               
               
                   
                 2nd 
                 103.5 
                 2.0 
                 91.2 
                 5.6 
                 16.3 
               
               
                   
                 3rd 
                 112.0 
                 2.0 
                 98.4 
                 5.6 
                 17.6 
               
               
                   
                 Mean 
                 105.0 
                 2.0 
                 92.5 
                 5.4 
                 17.1 
               
               
                   
                 STDER 
                 3.7 
                 0.0 
                 3.1 
                 0.2 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 101.5 
                 2.0 
                 89.5 
                 5.8 
                 15.4 
               
               
                   
                 2nd 
                 104.5 
                 2.0 
                 92.1 
                 4.8 
                 19.2 
               
               
                   
                 3rd 
                 106.0 
                 2.0 
                 93.3 
                 5.3 
                 17.6 
               
               
                   
                 Mean 
                 104.0 
                 2.0 
                 91.6 
                 5.3 
                 17.4 
               
               
                   
                 STDER 
                 1.3 
                 0.0 
                 1.1 
                 0.3 
                 1.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 116.5 
                 2.0 
                 102.1 
                 5.2 
                 19.6 
               
               
                   
                 2nd 
                 109.5 
                 2.0 
                 96.3 
                 5.4 
                 17.8 
               
               
                   
                 3rd 
                 119.5 
                 2.0 
                 104.6 
                 5.6 
                 18.7 
               
               
                   
                 Mean 
                 115.2 
                 2.0 
                 101.0 
                 5.4 
                 18.7 
               
               
                   
                 STDER 
                 3.0 
                 0.0 
                 2.5 
                 0.1 
                 0.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 162.5 
                 2.0 
                 140.1 
                 6.2 
                 22.6 
               
               
                   
                 2nd 
                 155.5 
                 2.0 
                 134.4 
                 6.4 
                 21.0 
               
               
                   
                 Mean 
                 159.0 
                 2.0 
                 137.2 
                 6.3 
                 21.8 
               
               
                   
                 STDER 
                 3.5 
                 0.0 
                 2.9 
                 0.1 
                 0.8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 42 
               
             
            
               
                   
               
               
                 Bead name: IL-2 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                 conc/ 
               
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.0 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 NaN 
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 11.5 
                 2.0 
                 2.3 
                 7.5 
                 0.3 
               
               
                   
                 2nd 
                 14.5 
                 2.0 
                 4.4 
                 7.4 
                 0.6 
               
               
                   
                 3rd 
                 12.0 
                 2.0 
                 2.6 
                 6.6 
                 0.4 
               
               
                   
                 Mean 
                 12.7 
                 2.0 
                 3.1 
                 7.2 
                 0.4 
               
               
                   
                 STDER 
                 0.9 
                 0.0 
                 0.7 
                 0.3 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 26.0 
                 2.0 
                 12.5 
                 6.1 
                 2.1 
               
               
                   
                 2nd 
                 23.5 
                 2.0 
                 10.8 
                 5.8 
                 1.9 
               
               
                   
                 3rd 
                 26.0 
                 2.0 
                 12.5 
                 5.5 
                 2.3 
               
               
                   
                 Mean 
                 25.2 
                 2.0 
                 11.9 
                 5.8 
                 2.1 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 0.6 
                 0.2 
                 0.1 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 92.5 
                 2.0 
                 56.9 
                 5.2 
                 10.9 
               
               
                   
                 2nd 
                 94.0 
                 2.0 
                 57.9 
                 4.8 
                 12.1 
               
               
                   
                 Mean 
                 93.3 
                 2.0 
                 57.4 
                 5.0 
                 11.5 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 0.5 
                 0.2 
                 0.6 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 75.0 
                 2.0 
                 45.4 
                 4.4 
                 10.3 
               
               
                   
                 2nd 
                 117.5 
                 2.0 
                 73.2 
                 5.8 
                 12.6 
               
               
                   
                 Mean 
                 96.3 
                 2.0 
                 59.3 
                 5.1 
                 11.5 
               
               
                   
                 STDER 
                 21.3 
                 0.0 
                 13.9 
                 0.7 
                 1.1 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 97.0 
                 2.0 
                 59.8 
                 5.5 
                 10.9 
               
               
                   
                 2nd 
                 106.0 
                 2.0 
                 65.7 
                 5.5 
                 11.9 
               
               
                   
                 Mean 
                 101.5 
                 2.0 
                 62.8 
                 5.5 
                 11.4 
               
               
                   
                 STDER 
                 4.5 
                 0.0 
                 2.9 
                 0.0 
                 0.5 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 112.0 
                 2.0 
                 69.6 
                 6.4 
                 10.9 
               
               
                   
                 2nd 
                 115.0 
                 2.0 
                 71.5 
                 6.1 
                 11.7 
               
               
                   
                 Mean 
                 113.5 
                 2.0 
                 70.6 
                 6.3 
                 11.3 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 1.0 
                 0.2 
                 0.4 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 27.0 
                 2.0 
                 13.2 
                 6.4 
                 2.1 
               
               
                   
                 2nd 
                 31.0 
                 2.0 
                 16.0 
                 7.0 
                 2.3 
               
               
                   
                 Mean 
                 29.0 
                 2.0 
                 14.6 
                 6.7 
                 2.2 
               
               
                   
                 STDER 
                 2.0 
                 0.0 
                 1.4 
                 0.3 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 25.0 
                 2.0 
                 11.8 
                 6.2 
                 1.9 
               
               
                   
                 2nd 
                 16.0 
                 2.0 
                 5.5 
                 7.6 
                 0.7 
               
               
                   
                 Mean 
                 20.5 
                 2.0 
                 8.7 
                 6.9 
                 1.3 
               
               
                   
                 STDER 
                 4.5 
                 0.0 
                 3.2 
                 0.7 
                 0.6 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 14.0 
                 2.0 
                 4.1 
                 5.2 
                 0.8 
               
               
                   
                 2nd 
                 9.0 
                 2.0 
                 0.4 
                 5.8 
                 0.1 
               
               
                   
                 Mean 
                 11.5 
                 2.0 
                 2.2 
                 5.5 
                 0.4 
               
               
                   
                 STDER 
                 2.5 
                 0.0 
                 1.9 
                 0.3 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 6.0 
                 2.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 2nd 
                 10.0 
                 2.0 
                 1.1 
                 5.7 
                 0.2 
               
               
                   
                 Mean 
                 8.0 
                 2.0 
                 1.1 
                 6.2 
                 0.1 
               
               
                   
                 STDER 
                 2.0 
                 0.0 
                 #DIV/0! 
                 0.5 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 6.0 
                 2.0 
                   
                 4.8 
                 0.0 
               
               
                   
                 2nd 
                 3.0 
                 2.0 
                   
                 4.4 
                 0.0 
               
               
                   
                 Mean 
                 4.5 
                 2.0 
                 #DIV/0! 
                 4.6 
                 0.0 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 #DIV/0! 
                 0.2 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 5.0 
                 2.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 2nd 
                 4.0 
                 2.0 
                   
                 6.4 
                 0.0 
               
               
                   
                 Mean 
                 4.5 
                 2.0 
                 #DIV/0! 
                 6.1 
                 0.0 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 2.0 
                 2.0 
                   
                 7.1 
                 0.0 
               
               
                   
                 2nd 
                 3.0 
                 2.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 Mean 
                 2.5 
                 2.0 
                 #DIV/0! 
                 6.5 
                 0.0 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 0.7 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 13.0 
                 2.0 
                 3.4 
                 5.7 
                 0.6 
               
               
                   
                 2nd 
                 19.0 
                 2.0 
                 7.6 
                 7.1 
                 1.1 
               
               
                   
                 3rd 
                 17.0 
                 2.0 
                 6.2 
                 6.6 
                 0.9 
               
               
                   
                 Mean 
                 16.3 
                 2.0 
                 5.7 
                 6.5 
                 0.9 
               
               
                   
                 STDER 
                 1.8 
                 0.0 
                 1.3 
                 0.4 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 18.0 
                 2.0 
                 6.9 
                 6.4 
                 1.1 
               
               
                   
                 2nd 
                 25.0 
                 2.0 
                 11.8 
                 6.0 
                 2.0 
               
               
                   
                 3rd 
                 18.0 
                 2.0 
                 6.9 
                 6.1 
                 1.1 
               
               
                   
                 Mean 
                 20.3 
                 2.0 
                 8.6 
                 6.2 
                 1.4 
               
               
                   
                 STDER 
                 2.3 
                 0.0 
                 1.6 
                 0.1 
                 0.3 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 8.0 
                 2.0 
                   
                 6.2 
                 0.0 
               
               
                   
                 2nd 
                 3.0 
                 2.0 
                   
                 5.2 
                 0.0 
               
               
                   
                 3rd 
                 8.0 
                 2.0 
                   
                 6.2 
                 0.0 
               
               
                   
                 Mean 
                 6.3 
                 2.0 
                 #DIV/0! 
                 5.9 
                 0.0 
               
               
                   
                 STDER 
                 1.7 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 15.5 
                 2.0 
                 5.2 
                 6.3 
                 0.8 
               
               
                   
                 2nd 
                 13.5 
                 2.0 
                 3.7 
                 7.1 
                 0.5 
               
               
                   
                 3rd 
                 12.0 
                 2.0 
                 2.6 
                 6.2 
                 0.4 
               
               
                   
                 Mean 
                 13.7 
                 2.0 
                 3.8 
                 6.5 
                 0.6 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 0.7 
                 0.3 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 19.5 
                 2.0 
                 8.0 
                 6.0 
                 1.3 
               
               
                   
                 2nd 
                 14.0 
                 2.0 
                 4.1 
                 6.4 
                 0.6 
               
               
                   
                 3rd 
                 13.5 
                 2.0 
                 3.7 
                 7.6 
                 0.5 
               
               
                   
                 Mean 
                 15.7 
                 2.0 
                 5.3 
                 6.7 
                 0.8 
               
               
                   
                 STDER 
                 1.9 
                 0.0 
                 1.4 
                 0.5 
                 0.3 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 11.0 
                 2.0 
                 1.9 
                 6.3 
                 0.3 
               
               
                   
                 2nd 
                 9.0 
                 2.0 
                 0.4 
                 6.2 
                 0.1 
               
               
                   
                 3rd 
                 7.0 
                 2.0 
                   
                 5.0 
                 0.0 
               
               
                   
                 Mean 
                 9.0 
                 2.0 
                 1.1 
                 5.8 
                 0.1 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 0.8 
                 0.4 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 4.0 
                 2.0 
                   
                 4.5 
                 0.0 
               
               
                   
                 2nd 
                 4.0 
                 2.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 3rd 
                 4.0 
                 2.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 Mean 
                 4.0 
                 2.0 
                 #DIV/0! 
                 5.4 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.4 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 5.0 
                 2.0 
                   
                 6.3 
                 0.0 
               
               
                   
                 2nd 
                 5.0 
                 2.0 
                   
                 6.2 
                 0.0 
               
               
                   
                 3rd 
                 5.0 
                 2.0 
                   
                 5.1 
                 0.0 
               
               
                   
                 Mean 
                 5.0 
                 2.0 
                 #DIV/0! 
                 5.9 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.4 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 7.0 
                 2.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 2nd 
                 8.0 
                 2.0 
                   
                 5.0 
                 0.0 
               
               
                   
                 3rd 
                 5.0 
                 2.0 
                   
                 5.7 
                 0.0 
               
               
                   
                 Mean 
                 6.7 
                 2.0 
                 #DIV/0! 
                 5.8 
                 0.0 
               
               
                   
                 STDER 
                 0.9 
                 0.0 
                 #DIV/0! 
                 0.5 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 6.0 
                 2.0 
                   
                 5.5 
                 0.0 
               
               
                   
                 2nd 
                 4.0 
                 2.0 
                   
                 4.8 
                 0.0 
               
               
                   
                 3rd 
                 6.0 
                 2.0 
                   
                 4.8 
                 0.0 
               
               
                   
                 Mean 
                 5.3 
                 2.0 
                 #DIV/0! 
                 5.0 
                 0.0 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 #DIV/0! 
                 0.2 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 6.0 
                 2.0 
                   
                 5.4 
                 0.0 
               
               
                   
                 2nd 
                 5.0 
                 2.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 3rd 
                 5.0 
                 2.0 
                   
                 4.7 
                 0.0 
               
               
                   
                 Mean 
                 5.3 
                 2.0 
                 #DIV/0! 
                 5.3 
                 0.0 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 4.0 
                 2.0 
                   
                 6.1 
                 0.0 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                   
                 6.1 
                 0.0 
               
               
                   
                 3rd 
                 6.0 
                 2.0 
                   
                 6.0 
                 0.0 
               
               
                   
                 Mean 
                 4.0 
                 2.0 
                 #DIV/0! 
                 6.1 
                 0.0 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 #DIV/0! 
                 0.0 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 3.0 
                 2.0 
                   
                 4.4 
                 0.0 
               
               
                   
                 2nd 
                 8.0 
                 2.0 
                   
                 6.0 
                 0.0 
               
               
                   
                 3rd 
                 8.0 
                 2.0 
                   
                 4.6 
                 0.0 
               
               
                   
                 Mean 
                 6.3 
                 2.0 
                 #DIV/0! 
                 5.0 
                 0.0 
               
               
                   
                 STDER 
                 1.7 
                 0.0 
                 #DIV/0! 
                 0.5 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 6.0 
                 2.0 
                   
                 4.5 
                 0.0 
               
               
                   
                 2nd 
                 4.0 
                 2.0 
                   
                 6.6 
                 0.0 
               
               
                   
                 3rd 
                 4.0 
                 2.0 
                   
                 5.0 
                 0.0 
               
               
                   
                 Mean 
                 4.7 
                 2.0 
                 #DIV/0! 
                 5.4 
                 0.0 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 #DIV/0! 
                 0.6 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 6.0 
                 2.0 
                   
                 5.0 
                 0.0 
               
               
                   
                 2nd 
                 3.0 
                 2.0 
                   
                 5.6 
                 0.0 
               
               
                   
                 3rd 
                 8.0 
                 2.0 
                   
                 5.6 
                 0.0 
               
               
                   
                 Mean 
                 5.7 
                 2.0 
                 #DIV/0! 
                 5.4 
                 0.0 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 #DIV/0! 
                 0.2 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 3.0 
                 2.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                   
                 4.8 
                 0.0 
               
               
                   
                 3rd 
                 4.0 
                 2.0 
                   
                 5.3 
                 0.0 
               
               
                   
                 Mean 
                 3.0 
                 2.0 
                 #DIV/0! 
                 5.3 
                 0.0 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 #DIV/0! 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 3.0 
                 2.0 
                   
                 5.2 
                 0.0 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                   
                 5.4 
                 0.0 
               
               
                   
                 3rd 
                 5.0 
                 2.0 
                   
                 5.6 
                 0.0 
               
               
                   
                 Mean 
                 3.3 
                 2.0 
                 #DIV/0! 
                 5.4 
                 0.0 
               
               
                   
                 STDER 
                 0.9 
                 0.0 
                 #DIV/0! 
                 0.1 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 2.0 
                 2.0 
                   
                 6.2 
                 0.0 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                   
                 6.4 
                 0.0 
               
               
                   
                 Mean 
                 2.0 
                 2.0 
                 #DIV/0! 
                 6.3 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.1 
                 0.0 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 43 
               
             
            
               
                   
               
               
                 Bead name: IL-17A 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                 conc/ 
               
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.5 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 −0.5 
                 2.0 
                 NaN 
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 0.5 
                 2.0 
                 1.0 
                 7.5 
                 0.1 
               
               
                   
                 2nd 
                 2.5 
                 2.0 
                 4.3 
                 7.4 
                 0.6 
               
               
                   
                 3rd 
                 1.5 
                 2.0 
                 2.7 
                 6.6 
                 0.4 
               
               
                   
                 Mean 
                 1.5 
                 2.0 
                 2.7 
                 7.2 
                 0.4 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 1.0 
                 0.3 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 4.5 
                 2.0 
                 7.5 
                 6.1 
                 1.2 
               
               
                   
                 2nd 
                 3.5 
                 2.0 
                 6.0 
                 5.8 
                 1.0 
               
               
                   
                 3rd 
                 4.5 
                 2.0 
                 7.5 
                 5.5 
                 1.4 
               
               
                   
                 Mean 
                 4.2 
                 2.0 
                 7.0 
                 5.8 
                 1.2 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.5 
                 0.2 
                 0.1 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 4.5 
                 2.0 
                 7.5 
                 5.2 
                 1.5 
               
               
                   
                 2nd 
                 4.5 
                 2.0 
                 7.5 
                 4.8 
                 1.6 
               
               
                   
                 Mean 
                 4.5 
                 2.0 
                 7.5 
                 5.0 
                 1.5 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.2 
                 0.1 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 2.5 
                 2.0 
                 4.3 
                 4.4 
                 1.0 
               
               
                   
                 2nd 
                 7.5 
                 2.0 
                 12.2 
                 5.8 
                 2.1 
               
               
                   
                 Mean 
                 5.0 
                 2.0 
                 8.3 
                 5.1 
                 1.5 
               
               
                   
                 STDER 
                 2.5 
                 0.0 
                 3.9 
                 0.7 
                 0.6 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 3.5 
                 2.0 
                 6.0 
                 5.5 
                 1.1 
               
               
                   
                 2nd 
                 2.5 
                 2.0 
                 4.3 
                 5.5 
                 0.8 
               
               
                   
                 Mean 
                 3.0 
                 2.0 
                 5.2 
                 5.5 
                 0.9 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 0.8 
                 0.0 
                 0.1 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 14.5 
                 2.0 
                 22.9 
                 6.4 
                 3.6 
               
               
                   
                 2nd 
                 8.5 
                 2.0 
                 13.8 
                 6.1 
                 2.3 
               
               
                   
                 Mean 
                 11.5 
                 2.0 
                 18.3 
                 6.3 
                 2.9 
               
               
                   
                 STDER 
                 3.0 
                 0.0 
                 4.6 
                 0.2 
                 0.7 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 1.5 
                 2.0 
                 2.7 
                 6.4 
                 0.4 
               
               
                   
                 2nd 
                 2.5 
                 2.0 
                 4.3 
                 7.0 
                 0.6 
               
               
                   
                 Mean 
                 2.0 
                 2.0 
                 3.5 
                 6.7 
                 0.5 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 0.8 
                 0.3 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 7.5 
                 2.0 
                 12.2 
                 6.2 
                 2.0 
               
               
                   
                 2nd 
                 2.5 
                 2.0 
                 4.3 
                 7.6 
                 0.6 
               
               
                   
                 Mean 
                 5.0 
                 2.0 
                 8.3 
                 6.9 
                 1.3 
               
               
                   
                 STDER 
                 2.5 
                 0.0 
                 3.9 
                 0.7 
                 0.7 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 3.5 
                 2.0 
                 6.0 
                 5.2 
                 1.1 
               
               
                   
                 2nd 
                 3.5 
                 2.0 
                 6.0 
                 5.8 
                 1.0 
               
               
                   
                 Mean 
                 3.5 
                 2.0 
                 6.0 
                 5.5 
                 1.1 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.3 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 3.5 
                 2.0 
                 6.0 
                 6.6 
                 0.9 
               
               
                   
                 2nd 
                 2.5 
                 2.0 
                 4.3 
                 5.7 
                 0.8 
               
               
                   
                 Mean 
                 3.0 
                 2.0 
                 5.2 
                 6.2 
                 0.8 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 0.8 
                 0.5 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 0.5 
                 2.0 
                 1.0 
                 4.8 
                 0.2 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                 1.9 
                 4.4 
                 0.4 
               
               
                   
                 Mean 
                 0.8 
                 2.0 
                 1.5 
                 4.6 
                 0.3 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.4 
                 0.2 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 1.5 
                 2.0 
                 2.7 
                 5.8 
                 0.5 
               
               
                   
                 2nd 
                 1.5 
                 2.0 
                 2.7 
                 6.4 
                 0.4 
               
               
                   
                 Mean 
                 1.5 
                 2.0 
                 2.7 
                 6.1 
                 0.4 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 1.5 
                 2.0 
                 2.7 
                 7.1 
                 0.4 
               
               
                   
                 2nd 
                 1.5 
                 2.0 
                 2.7 
                 5.8 
                 0.5 
               
               
                   
                 Mean 
                 1.5 
                 2.0 
                 2.7 
                 6.5 
                 0.4 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.7 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 2.5 
                 2.0 
                 4.3 
                 5.7 
                 0.8 
               
               
                   
                 2nd 
                 2.5 
                 2.0 
                 4.3 
                 7.1 
                 0.6 
               
               
                   
                 3rd 
                 1.5 
                 2.0 
                 2.7 
                 6.6 
                 0.4 
               
               
                   
                 Mean 
                 2.2 
                 2.0 
                 3.8 
                 6.5 
                 0.6 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.5 
                 0.4 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 3.5 
                 2.0 
                 6.0 
                 6.4 
                 0.9 
               
               
                   
                 2nd 
                 3.5 
                 2.0 
                 6.0 
                 6.0 
                 1.0 
               
               
                   
                 3rd 
                 1.5 
                 2.0 
                 2.7 
                 6.1 
                 0.4 
               
               
                   
                 Mean 
                 2.8 
                 2.0 
                 4.9 
                 6.2 
                 0.8 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 1.1 
                 0.1 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 3.5 
                 2.0 
                 6.0 
                 6.2 
                 1.0 
               
               
                   
                 2nd 
                 0.5 
                 2.0 
                 1.0 
                 5.2 
                 0.2 
               
               
                   
                 3rd 
                 3.5 
                 2.0 
                 6.0 
                 6.2 
                 1.0 
               
               
                   
                 Mean 
                 2.5 
                 2.0 
                 4.3 
                 5.9 
                 0.7 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 1.6 
                 0.3 
                 0.3 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 2.5 
                 2.0 
                 4.3 
                 6.3 
                 0.7 
               
               
                   
                 2nd 
                 2.5 
                 2.0 
                 4.3 
                 7.1 
                 0.6 
               
               
                   
                 3rd 
                 4.5 
                 2.0 
                 7.5 
                 6.2 
                 1.2 
               
               
                   
                 Mean 
                 3.2 
                 2.0 
                 5.4 
                 6.5 
                 0.8 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 1.1 
                 0.3 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 2.5 
                 2.0 
                 4.3 
                 6.0 
                 0.7 
               
               
                   
                 2nd 
                 1.5 
                 2.0 
                 2.7 
                 6.4 
                 0.4 
               
               
                   
                 3rd 
                 1.5 
                 2.0 
                 2.7 
                 7.6 
                 0.4 
               
               
                   
                 Mean 
                 1.8 
                 2.0 
                 3.3 
                 6.7 
                 0.5 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.5 
                 0.5 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 0.5 
                 2.0 
                 1.0 
                 6.3 
                 0.2 
               
               
                   
                 2nd 
                 1.5 
                 2.0 
                 2.7 
                 6.2 
                 0.4 
               
               
                   
                 3rd 
                 0.5 
                 2.0 
                 1.0 
                 5.0 
                 0.2 
               
               
                   
                 Mean 
                 0.8 
                 2.0 
                 1.6 
                 5.8 
                 0.3 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.6 
                 0.4 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 2.5 
                 2.0 
                 4.3 
                 4.5 
                 1.0 
               
               
                   
                 2nd 
                 1.5 
                 2.0 
                 2.7 
                 5.8 
                 0.5 
               
               
                   
                 3rd 
                 1.5 
                 2.0 
                 2.7 
                 5.8 
                 0.5 
               
               
                   
                 Mean 
                 1.8 
                 2.0 
                 3.3 
                 5.4 
                 0.6 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.5 
                 0.4 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 0.5 
                 2.0 
                 1.0 
                 6.3 
                 0.2 
               
               
                   
                 2nd 
                 0.5 
                 2.0 
                 1.0 
                 6.2 
                 0.2 
               
               
                   
                 3rd 
                 2.5 
                 2.0 
                 4.3 
                 5.1 
                 0.9 
               
               
                   
                 Mean 
                 1.2 
                 2.0 
                 2.1 
                 5.9 
                 0.4 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 1.1 
                 0.4 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 1.5 
                 2.0 
                 2.7 
                 6.6 
                 0.4 
               
               
                   
                 2nd 
                 0.5 
                 2.0 
                 1.0 
                 5.0 
                 0.2 
               
               
                   
                 3rd 
                 0.5 
                 2.0 
                 1.0 
                 5.7 
                 0.2 
               
               
                   
                 Mean 
                 0.8 
                 2.0 
                 1.6 
                 5.8 
                 0.3 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.6 
                 0.5 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 luM 
                 1st 
                 2.5 
                 2.0 
                 4.3 
                 5.5 
                 0.8 
               
               
                   
                 2nd 
                 2.5 
                 2.0 
                 4.3 
                 4.8 
                 0.9 
               
               
                   
                 3rd 
                 1.5 
                 2.0 
                 2.7 
                 4.8 
                 0.6 
               
               
                   
                 Mean 
                 2.2 
                 2.0 
                 3.8 
                 5.0 
                 0.8 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.5 
                 0.2 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 1.5 
                 2.0 
                 2.7 
                 5.4 
                 0.5 
               
               
                   
                 2nd 
                 1.5 
                 2.0 
                 2.7 
                 5.8 
                 0.5 
               
               
                   
                 3rd 
                 −0.5 
                 2.0 
                   
                 4.7 
                 0.0 
               
               
                   
                 Mean 
                 0.8 
                 2.0 
                 2.7 
                 5.3 
                 0.3 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 0.0 
                 0.3 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 0.5 
                 2.0 
                 1.0 
                 6.1 
                 0.2 
               
               
                   
                 2nd 
                 0.5 
                 2.0 
                 1.0 
                 6.1 
                 0.2 
               
               
                   
                 3rd 
                 −0.5 
                 2.0 
                   
                 6.0 
                 0.0 
               
               
                   
                 Mean 
                 0.2 
                 2.0 
                 1.0 
                 6.1 
                 0.1 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.0 
                 0.0 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 0.5 
                 2.0 
                 1.0 
                 4.4 
                 0.2 
               
               
                   
                 2nd 
                 −0.5 
                 2.0 
                   
                 6.0 
                 0.0 
               
               
                   
                 3rd 
                 0.5 
                 2.0 
                 1.0 
                 4.6 
                 0.2 
               
               
                   
                 Mean 
                 0.2 
                 2.0 
                 1.0 
                 5.0 
                 0.2 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.0 
                 0.5 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 0.5 
                 2.0 
                 1.0 
                 4.5 
                 0.2 
               
               
                   
                 2nd 
                 0.5 
                 2.0 
                 1.0 
                 6.6 
                 0.2 
               
               
                   
                 3rd 
                 0.5 
                 2.0 
                 1.0 
                 5.0 
                 0.2 
               
               
                   
                 Mean 
                 0.5 
                 2.0 
                 1.0 
                 5.4 
                 0.2 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.6 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 1.5 
                 2.0 
                 2.7 
                 5.0 
                 0.5 
               
               
                   
                 2nd 
                 0.5 
                 2.0 
                 1.0 
                 5.6 
                 0.2 
               
               
                   
                 3rd 
                 0.5 
                 2.0 
                 1.0 
                 5.6 
                 0.2 
               
               
                   
                 Mean 
                 0.8 
                 2.0 
                 1.6 
                 5.4 
                 0.3 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.6 
                 0.2 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 0.5 
                 2.0 
                 1.0 
                 5.8 
                 0.2 
               
               
                   
                 2nd 
                 −0.5 
                 2.0 
                   
                 4.8 
                 0.0 
               
               
                   
                 3rd 
                 1.5 
                 2.0 
                 2.7 
                 5.3 
                 0.5 
               
               
                   
                 Mean 
                 0.5 
                 2.0 
                 1.9 
                 5.3 
                 0.2 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 0.8 
                 0.3 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 0.0 
                 2.0 
                 0.1 
                 5.2 
                 0.0 
               
               
                   
                 2nd 
                 1.5 
                 2.0 
                 2.7 
                 5.4 
                 0.5 
               
               
                   
                 3rd 
                 0.5 
                 2.0 
                 1.0 
                 5.6 
                 0.2 
               
               
                   
                 Mean 
                 0.7 
                 2.0 
                 1.3 
                 5.4 
                 0.2 
               
               
                   
                 STDER 
                 0.4 
                 0.0 
                 0.8 
                 0.1 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 −0.5 
                 2.0 
                   
                 6.2 
                 0.0 
               
               
                   
                 2nd 
                 −0.5 
                 2.0 
                   
                 6.4 
                 0.0 
               
               
                   
                 Mean 
                 −0.5 
                 2.0 
                 #DIV/0! 
                 6.3 
                 0.0 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 0.1 
                 0.0 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
             
               
                 TABLE 44 
               
               
                   
               
               
                   
                   
                   
                   
                   
                   
                 conc/ 
               
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Bead name: IL-4 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 0.0 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 NaN 
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 −1.0 
                 2.0 
                 7.7 
                 7.5 
                 1.0 
               
               
                   
                 2nd 
                 −0.5 
                 2.0 
                 13.6 
                 7.4 
                 1.8 
               
               
                   
                 3rd 
                 −1.0 
                 2.0 
                 7.7 
                 6.6 
                 1.2 
               
               
                   
                 Mean 
                 −0.8 
                 2.0 
                 9.7 
                 7.2 
                 1.3 
               
               
                   
                 STDER 
                 0.2 
                 0.0 
                 2.0 
                 0.3 
                 0.3 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 2.0 
                 2.0 
                 33.2 
                 6.1 
                 5.4 
               
               
                   
                 2nd 
                 3.0 
                 2.0 
                 39.4 
                 5.8 
                 6.8 
               
               
                   
                 3rd 
                 2.0 
                 2.0 
                 33.2 
                 5.5 
                 6.0 
               
               
                   
                 Mean 
                 2.3 
                 2.0 
                 35.3 
                 5.8 
                 6.1 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 2.1 
                 0.2 
                 0.4 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 0.5 
                 2.0 
                 22.6 
                 5.2 
                 4.3 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                 33.2 
                 4.8 
                 6.9 
               
               
                   
                 Mean 
                 1.3 
                 2.0 
                 27.9 
                 5.0 
                 5.6 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 5.3 
                 0.2 
                 1.3 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 1.0 
                 2.0 
                 26.4 
                 4.4 
                 6.0 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                 33.2 
                 5.8 
                 5.7 
               
               
                   
                 Mean 
                 1.5 
                 2.0 
                 29.8 
                 5.1 
                 5.9 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 3.4 
                 0.7 
                 0.1 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 0.0 
                 2.0 
                 18.4 
                 5.5 
                 3.3 
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 18.4 
                 5.5 
                 3.3 
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 18.4 
                 5.5 
                 3.3 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.0 
                 0.0 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 6.0 
                 2.0 
                 55.5 
                 6.4 
                 8.7 
               
               
                   
                 2nd 
                 3.0 
                 2.0 
                 39.4 
                 6.1 
                 6.5 
               
               
                   
                 Mean 
                 4.5 
                 2.0 
                 47.4 
                 6.3 
                 7.6 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 8.1 
                 0.2 
                 1.1 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 −1.0 
                 2.0 
                 7.7 
                 6.4 
                 1.2 
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 18.4 
                 7.0 
                 2.6 
               
               
                   
                 Mean 
                 −0.5 
                 2.0 
                 13.0 
                 6.7 
                 1.9 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 5.3 
                 0.3 
                 0.7 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 2.0 
                 2.0 
                 33.2 
                 6.2 
                 5.4 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                 33.2 
                 7.6 
                 4.4 
               
               
                   
                 Mean 
                 2.0 
                 2.0 
                 33.2 
                 6.9 
                 4.9 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.7 
                 0.5 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 2.0 
                 2.0 
                 33.2 
                 5.2 
                 6.4 
               
               
                   
                 2nd 
                 3.0 
                 2.0 
                 39.4 
                 5.8 
                 6.8 
               
               
                   
                 Mean 
                 2.5 
                 2.0 
                 36.3 
                 5.5 
                 6.6 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 3.1 
                 0.3 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 1.0 
                 2.0 
                 26.4 
                 6.6 
                 4.0 
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 18.4 
                 5.7 
                 3.2 
               
               
                   
                 Mean 
                 0.5 
                 2.0 
                 22.4 
                 6.2 
                 3.6 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 4.0 
                 0.5 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 2.0 
                 2.0 
                 33.2 
                 4.8 
                 6.9 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                 26.4 
                 4.4 
                 6.0 
               
               
                   
                 Mean 
                 1.5 
                 2.0 
                 29.8 
                 4.6 
                 6.5 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 3.4 
                 0.2 
                 0.5 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 2.0 
                 2.0 
                 33.2 
                 5.8 
                 5.7 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                 26.4 
                 6.4 
                 4.1 
               
               
                   
                 Mean 
                 1.5 
                 2.0 
                 29.8 
                 6.1 
                 4.9 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 3.4 
                 0.3 
                 0.8 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 0.0 
                 2.0 
                 18.4 
                 7.1 
                 2.6 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                 33.2 
                 5.8 
                 5.7 
               
               
                   
                 Mean 
                 1.0 
                 2.0 
                 25.8 
                 6.5 
                 4.2 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 7.4 
                 0.7 
                 1.6 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 0.0 
                 2.0 
                 18.4 
                 5.7 
                 3.2 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                 33.2 
                 7.1 
                 4.7 
               
               
                   
                 3rd 
                 1.0 
                 2.0 
                 26.4 
                 6.6 
                 4.0 
               
               
                   
                 Mean 
                 1.0 
                 2.0 
                 26.0 
                 6.5 
                 4.0 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 4.3 
                 0.4 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 2.0 
                 2.0 
                 33.2 
                 6.4 
                 5.2 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                 33.2 
                 6.0 
                 5.5 
               
               
                   
                 3rd 
                 2.0 
                 2.0 
                 33.2 
                 6.1 
                 5.4 
               
               
                   
                 Mean 
                 2.0 
                 2.0 
                 33.2 
                 6.2 
                 5.4 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.1 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 3.0 
                 2.0 
                 39.4 
                 6.2 
                 6.4 
               
               
                   
                 2nd 
                 4.0 
                 2.0 
                 45.1 
                 5.2 
                 8.7 
               
               
                   
                 3rd 
                 2.0 
                 2.0 
                 33.2 
                 6.2 
                 5.4 
               
               
                   
                 Mean 
                 3.0 
                 2.0 
                 39.2 
                 5.9 
                 6.8 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 3.4 
                 0.3 
                 1.0 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 2.0 
                 2.0 
                 33.2 
                 6.3 
                 5.3 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                 33.2 
                 7.1 
                 4.7 
               
               
                   
                 3rd 
                 2.0 
                 2.0 
                 33.2 
                 6.2 
                 5.4 
               
               
                   
                 Mean 
                 2.0 
                 2.0 
                 33.2 
                 6.5 
                 5.1 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.3 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 1.0 
                 2.0 
                 26.4 
                 6.0 
                 4.4 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                 26.4 
                 6.4 
                 4.1 
               
               
                   
                 3rd 
                 0.0 
                 2.0 
                 18.4 
                 7.6 
                 2.4 
               
               
                   
                 Mean 
                 0.7 
                 2.0 
                 23.7 
                 6.7 
                 3.6 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 2.7 
                 0.5 
                 0.6 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 0.0 
                 2.0 
                 18.4 
                 6.3 
                 2.9 
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 18.4 
                 6.2 
                 3.0 
               
               
                   
                 3rd 
                 −1.0 
                 2.0 
                 7.7 
                 5.0 
                 1.5 
               
               
                   
                 Mean 
                 −0.3 
                 2.0 
                 14.8 
                 5.8 
                 2.5 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 3.6 
                 0.4 
                 0.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 0.0 
                 2.0 
                 18.4 
                 4.5 
                 4.1 
               
               
                   
                 2nd 
                 −1.0 
                 2.0 
                 7.7 
                 5.8 
                 1.3 
               
               
                   
                 3rd 
                 −0.5 
                 2.0 
                 13.6 
                 5.8 
                 2.3 
               
               
                   
                 Mean 
                 −0.5 
                 2.0 
                 13.2 
                 5.4 
                 2.6 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 3.1 
                 0.4 
                 0.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 0.0 
                 2.0 
                 18.4 
                 6.3 
                 2.9 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                 26.4 
                 6.2 
                 4.3 
               
               
                   
                 3rd 
                 1.0 
                 2.0 
                 26.4 
                 5.1 
                 5.2 
               
               
                   
                 Mean 
                 0.7 
                 2.0 
                 23.7 
                 5.9 
                 4.1 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 2.7 
                 0.4 
                 0.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 0.0 
                 2.0 
                 18.4 
                 6.6 
                 2.8 
               
               
                   
                 2nd 
                 −2.0 
                 2.0 
                   
                 5.0 
                 0.0 
               
               
                   
                 3rd 
                 1.0 
                 2.0 
                 26.4 
                 5.7 
                 4.6 
               
               
                   
                 Mean 
                 −0.3 
                 2.0 
                 22.4 
                 5.8 
                 2.5 
               
               
                   
                 STDER 
                 0.9 
                 0.0 
                 4.0 
                 0.5 
                 1.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 0.0 
                 2.0 
                 18.4 
                 5.5 
                 3.3 
               
               
                   
                 2nd 
                 −1.5 
                 2.0 
                   
                 4.8 
                 0.0 
               
               
                   
                 3rd 
                 0.0 
                 2.0 
                 18.4 
                 4.8 
                 3.8 
               
               
                   
                 Mean 
                 −0.5 
                 2.0 
                 18.4 
                 5.0 
                 2.4 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 0.0 
                 0.2 
                 1.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 0.0 
                 2.0 
                 18.4 
                 5.4 
                 3.4 
               
               
                   
                 2nd 
                 −0.5 
                 2.0 
                 13.6 
                 5.8 
                 2.3 
               
               
                   
                 3rd 
                 0.0 
                 2.0 
                 18.4 
                 4.7 
                 3.9 
               
               
                   
                 Mean 
                 −0.2 
                 2.0 
                 16.8 
                 5.3 
                 3.2 
               
               
                   
                 STDER 
                 0.2 
                 0.0 
                 1.6 
                 0.3 
                 0.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 −1.0 
                 2.0 
                 7.7 
                 6.1 
                 1.3 
               
               
                   
                 2nd 
                 −1.5 
                 2.0 
                   
                 6.1 
                 0.0 
               
               
                   
                 3rd 
                 −1.0 
                 2.0 
                 7.7 
                 6.0 
                 1.3 
               
               
                   
                 Mean 
                 −1.2 
                 2.0 
                 7.7 
                 6.1 
                 0.8 
               
               
                   
                 STDER 
                 0.2 
                 0.0 
                 0.0 
                 0.0 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 1.0 
                 2.0 
                 26.4 
                 4.4 
                 6.0 
               
               
                   
                 2nd 
                 2.0 
                 2.0 
                 33.2 
                 6.0 
                 5.5 
               
               
                   
                 3rd 
                 0.0 
                 2.0 
                 18.4 
                 4.6 
                 4.0 
               
               
                   
                 Mean 
                 1.0 
                 2.0 
                 26.0 
                 5.0 
                 5.2 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 4.3 
                 0.5 
                 0.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 −1.0 
                 2.0 
                 7.7 
                 4.5 
                 1.7 
               
               
                   
                 2nd 
                 −1.0 
                 2.0 
                 7.7 
                 6.6 
                 1.2 
               
               
                   
                 3rd 
                 0.0 
                 2.0 
                 18.4 
                 5.0 
                 3.7 
               
               
                   
                 Mean 
                 −0.7 
                 2.0 
                 11.3 
                 5.4 
                 2.2 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 3.6 
                 0.6 
                 0.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 1.0 
                 2.0 
                 26.4 
                 5.0 
                 5.3 
               
               
                   
                 2nd 
                 0.0 
                 2.0 
                 18.4 
                 5.6 
                 3.3 
               
               
                   
                 3rd 
                 −1.0 
                 2.0 
                 7.7 
                 5.6 
                 1.4 
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 17.5 
                 5.4 
                 3.3 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 5.4 
                 0.2 
                 1.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 −1.5 
                 2.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 2nd 
                 1.0 
                 2.0 
                 26.4 
                 4.8 
                 5.5 
               
               
                   
                 3rd 
                 1.0 
                 2.0 
                 26.4 
                 5.3 
                 5.0 
               
               
                   
                 Mean 
                 0.2 
                 2.0 
                 26.4 
                 5.3 
                 3.5 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 0.0 
                 0.3 
                 1.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 0.0 
                 2.0 
                 18.4 
                 5.2 
                 3.5 
               
               
                   
                 2nd 
                 −1.0 
                 2.0 
                 7.7 
                 5.4 
                 1.4 
               
               
                   
                 3rd 
                 0.0 
                 2.0 
                 18.4 
                 5.6 
                 3.3 
               
               
                   
                 Mean 
                 −0.3 
                 2.0 
                 14.8 
                 5.4 
                 2.7 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 3.6 
                 0.1 
                 0.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 −1.0 
                 2.0 
                 7.7 
                 6.2 
                 1.2 
               
               
                   
                 2nd 
                 −1.0 
                 2.0 
                 7.7 
                 6.4 
                 1.2 
               
               
                   
                 Mean 
                 −1.0 
                 2.0 
                 7.7 
                 6.3 
                 1.2 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.1 
                 0.0 
               
            
           
           
               
            
               
                 Bead name: VEGF-A 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 −0.3 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.3 
                 2.0 
                 NaN 
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 2.3 
                 2.0 
                 10.7 
                 7.5 
                 1.4 
               
               
                   
                 2nd 
                 2.3 
                 2.0 
                 10.7 
                 7.4 
                 1.4 
               
               
                   
                 3rd 
                 1.3 
                 2.0 
                 8.5 
                 6.6 
                 1.3 
               
               
                   
                 Mean 
                 1.9 
                 2.0 
                 10.0 
                 7.2 
                 1.4 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.7 
                 0.3 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 0.3 
                 2.0 
                 6.1 
                 6.1 
                 1.0 
               
               
                   
                 2nd 
                 0.3 
                 2.0 
                 6.1 
                 5.8 
                 1.1 
               
               
                   
                 3rd 
                 −0.8 
                 2.0 
                 3.6 
                 5.5 
                 0.7 
               
               
                   
                 Mean 
                 −0.1 
                 2.0 
                 5.3 
                 5.8 
                 0.9 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.8 
                 0.2 
                 0.1 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 2.3 
                 2.0 
                 10.7 
                 5.2 
                 2.1 
               
               
                   
                 2nd 
                 0.3 
                 2.0 
                 6.1 
                 4.8 
                 1.3 
               
               
                   
                 Mean 
                 1.3 
                 2.0 
                 8.4 
                 5.0 
                 1.7 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 2.3 
                 0.2 
                 0.4 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 0.3 
                 2.0 
                 6.1 
                 4.4 
                 1.4 
               
               
                   
                 2nd 
                 1.3 
                 2.0 
                 8.5 
                 5.8 
                 1.5 
               
               
                   
                 Mean 
                 0.8 
                 2.0 
                 7.3 
                 5.1 
                 1.4 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 1.2 
                 0.7 
                 0.0 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 1.3 
                 2.0 
                 8.5 
                 5.5 
                 1.5 
               
               
                   
                 2nd 
                 0.3 
                 2.0 
                 6.1 
                 5.5 
                 1.1 
               
               
                   
                 Mean 
                 0.8 
                 2.0 
                 7.3 
                 5.5 
                 1.3 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 1.2 
                 0.0 
                 0.2 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 0.8 
                 2.0 
                 7.3 
                 6.4 
                 1.1 
               
               
                   
                 2nd 
                 1.3 
                 2.0 
                 8.5 
                 6.1 
                 1.4 
               
               
                   
                 Mean 
                 1.0 
                 2.0 
                 7.9 
                 6.3 
                 1.3 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.6 
                 0.2 
                 0.1 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 −0.8 
                 2.0 
                 3.6 
                 6.4 
                 0.6 
               
               
                   
                 2nd 
                 −0.8 
                 2.0 
                 3.6 
                 7.0 
                 0.5 
               
               
                   
                 Mean 
                 −0.8 
                 2.0 
                 3.6 
                 6.7 
                 0.5 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.3 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 0.3 
                 2.0 
                 6.1 
                 6.2 
                 1.0 
               
               
                   
                 2nd 
                 −0.8 
                 2.0 
                 3.6 
                 7.6 
                 0.5 
               
               
                   
                 Mean 
                 −0.3 
                 2.0 
                 4.9 
                 6.9 
                 0.7 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 1.3 
                 0.7 
                 0.3 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 −0.3 
                 2.0 
                 4.9 
                 5.2 
                 0.9 
               
               
                   
                 2nd 
                 −0.8 
                 2.0 
                 3.6 
                 5.8 
                 0.6 
               
               
                   
                 Mean 
                 −0.5 
                 2.0 
                 4.2 
                 5.5 
                 0.8 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.6 
                 0.3 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 0.3 
                 2.0 
                 6.1 
                 6.6 
                 0.9 
               
               
                   
                 2nd 
                 −2.8 
                 2.0 
                   
                 5.7 
                 0.0 
               
               
                   
                 Mean 
                 −1.3 
                 2.0 
                 6.1 
                 6.2 
                 0.5 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 #DIV/0! 
                 0.5 
                 0.5 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 −0.8 
                 2.0 
                 3.6 
                 4.8 
                 0.7 
               
               
                   
                 2nd 
                 −2.3 
                 2.0 
                   
                 4.4 
                 0.0 
               
               
                   
                 Mean 
                 −1.5 
                 2.0 
                 3.6 
                 4.6 
                 0.4 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 #DIV/0! 
                 0.2 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 −0.8 
                 2.0 
                 3.6 
                 5.8 
                 0.6 
               
               
                   
                 2nd 
                 −1.8 
                 2.0 
                 0.6 
                 6.4 
                 0.1 
               
               
                   
                 Mean 
                 −1.3 
                 2.0 
                 2.1 
                 6.1 
                 0.4 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 1.5 
                 0.3 
                 0.3 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 −0.8 
                 2.0 
                 3.6 
                 7.1 
                 0.5 
               
               
                   
                 2nd 
                 −0.8 
                 2.0 
                 3.6 
                 5.8 
                 0.6 
               
               
                   
                 Mean 
                 −0.8 
                 2.0 
                 3.6 
                 6.5 
                 0.6 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.7 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 −1.3 
                 2.0 
                 2.2 
                 5.7 
                 0.4 
               
               
                   
                 2nd 
                 0.3 
                 2.0 
                 6.1 
                 7.1 
                 0.9 
               
               
                   
                 3rd 
                 −1.8 
                 2.0 
                 0.6 
                 6.6 
                 0.1 
               
               
                   
                 Mean 
                 −0.9 
                 2.0 
                 3.0 
                 6.5 
                 0.4 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 1.6 
                 0.4 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 −1.8 
                 2.0 
                 0.6 
                 6.4 
                 0.1 
               
               
                   
                 2nd 
                 −3.8 
                 2.0 
                   
                 6.0 
                 0.0 
               
               
                   
                 3rd 
                 −3.8 
                 2.0 
                   
                 6.1 
                 0.0 
               
               
                   
                 Mean 
                 −3.1 
                 2.0 
                 0.6 
                 6.2 
                 0.0 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 #DIV/0! 
                 0.1 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 1st 
                 −0.8 
                 2.0 
                 3.6 
                 6.2 
                 0.6 
               
               
                   
                 2nd 
                 −1.8 
                 2.0 
                 0.6 
                 5.2 
                 0.1 
               
               
                   
                 3rd 
                 −0.8 
                 2.0 
                 3.6 
                 6.2 
                 0.6 
               
               
                   
                 Mean 
                 −1.1 
                 2.0 
                 2.6 
                 5.9 
                 0.4 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 1.0 
                 0.3 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 −2.3 
                 2.0 
                   
                 6.3 
                 0.0 
               
               
                   
                 2nd 
                 −1.3 
                 2.0 
                 2.2 
                 7.1 
                 0.3 
               
               
                   
                 3rd 
                 −0.8 
                 2.0 
                 3.6 
                 6.2 
                 0.6 
               
               
                   
                 Mean 
                 −1.4 
                 2.0 
                 2.9 
                 6.5 
                 0.3 
               
               
                   
                 STDER 
                 0.4 
                 0.0 
                 0.7 
                 0.3 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 −1.3 
                 2.0 
                 2.2 
                 6.0 
                 0.4 
               
               
                   
                 2nd 
                 −1.3 
                 2.0 
                 2.2 
                 6.4 
                 0.3 
               
               
                   
                 3rd 
                 −0.8 
                 2.0 
                 3.6 
                 7.6 
                 0.5 
               
               
                   
                 Mean 
                 −1.1 
                 2.0 
                 2.7 
                 6.7 
                 0.4 
               
               
                   
                 STDER 
                 0.2 
                 0.0 
                 0.5 
                 0.5 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 0.3 
                 2.0 
                 6.1 
                 6.3 
                 1.0 
               
               
                   
                 2nd 
                 0.3 
                 2.0 
                 6.1 
                 6.2 
                 1.0 
               
               
                   
                 3rd 
                 −1.8 
                 2.0 
                 0.6 
                 5.0 
                 0.1 
               
               
                   
                 Mean 
                 −0.4 
                 2.0 
                 4.3 
                 5.8 
                 0.7 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 1.8 
                 0.4 
                 0.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 0.3 
                 2.0 
                 6.1 
                 4.5 
                 1.4 
               
               
                   
                 2nd 
                 −1.8 
                 2.0 
                 0.6 
                 5.8 
                 0.1 
               
               
                   
                 3rd 
                 0.3 
                 2.0 
                 6.1 
                 5.8 
                 1.1 
               
               
                   
                 Mean 
                 −0.4 
                 2.0 
                 4.3 
                 5.4 
                 0.8 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 1.8 
                 0.4 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 −0.3 
                 2.0 
                 4.9 
                 6.3 
                 0.8 
               
               
                   
                 2nd 
                 −2.8 
                 2.0 
                   
                 6.2 
                 0.0 
               
               
                   
                 3rd 
                 −1.8 
                 2.0 
                 0.6 
                 5.1 
                 0.1 
               
               
                   
                 Mean 
                 −1.6 
                 2.0 
                 2.8 
                 5.9 
                 0.3 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 2.1 
                 0.4 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 −1.8 
                 2.0 
                 0.6 
                 6.6 
                 0.1 
               
               
                   
                 2nd 
                 −2.8 
                 2.0 
                   
                 5.0 
                 0.0 
               
               
                   
                 3rd 
                 −1.8 
                 2.0 
                 0.6 
                 5.7 
                 0.1 
               
               
                   
                 Mean 
                 −2.1 
                 2.0 
                 0.6 
                 5.8 
                 0.1 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.0 
                 0.5 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 −0.3 
                 2.0 
                 4.9 
                 5.5 
                 0.9 
               
               
                   
                 2nd 
                 −2.8 
                 2.0 
                   
                 4.8 
                 0.0 
               
               
                   
                 3rd 
                 −1.8 
                 2.0 
                 0.6 
                 4.8 
                 0.1 
               
               
                   
                 Mean 
                 −1.6 
                 2.0 
                 2.8 
                 5.0 
                 0.3 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 2.1 
                 0.2 
                 0.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 −0.8 
                 2.0 
                 3.6 
                 5.4 
                 0.7 
               
               
                   
                 2nd 
                 −2.8 
                 2.0 
                   
                 5.8 
                 0.0 
               
               
                   
                 3rd 
                 −1.8 
                 2.0 
                 0.6 
                 4.7 
                 0.1 
               
               
                   
                 Mean 
                 −1.8 
                 2.0 
                 2.1 
                 5.3 
                 0.3 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 1.5 
                 0.3 
                 0.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 −2.8 
                 2.0 
                   
                 6.1 
                 0.0 
               
               
                   
                 2nd 
                 −2.3 
                 2.0 
                   
                 6.1 
                 0.0 
               
               
                   
                 3rd 
                 −2.3 
                 2.0 
                   
                 6.0 
                 0.0 
               
               
                   
                 Mean 
                 −2.4 
                 2.0 
                 #DIV/0! 
                 6.1 
                 0.0 
               
               
                   
                 STDER 
                 0.2 
                 0.0 
                 #DIV/0! 
                 0.0 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 −0.8 
                 2.0 
                 3.6 
                 4.4 
                 0.8 
               
               
                   
                 2nd 
                 −0.8 
                 2.0 
                 3.6 
                 6.0 
                 0.6 
               
               
                   
                 3rd 
                 −0.8 
                 2.0 
                 3.6 
                 4.6 
                 0.8 
               
               
                   
                 Mean 
                 −0.8 
                 2.0 
                 3.6 
                 5.0 
                 0.7 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.5 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 −1.8 
                 2.0 
                 0.6 
                 4.5 
                 0.1 
               
               
                   
                 2nd 
                 −1.8 
                 2.0 
                 0.6 
                 6.6 
                 0.1 
               
               
                   
                 3rd 
                 −1.8 
                 2.0 
                 0.6 
                 5.0 
                 0.1 
               
               
                   
                 Mean 
                 −1.8 
                 2.0 
                 0.6 
                 5.4 
                 0.1 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.6 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 −1.8 
                 2.0 
                 0.6 
                 5.0 
                 0.1 
               
               
                   
                 2nd 
                 −1.8 
                 2.0 
                 0.6 
                 5.6 
                 0.1 
               
               
                   
                 3rd 
                 −1.8 
                 2.0 
                 0.6 
                 5.6 
                 0.1 
               
               
                   
                 Mean 
                 −1.8 
                 2.0 
                 0.6 
                 5.4 
                 0.1 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.2 
                 0.0 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 0.3 
                 2.0 
                 6.1 
                 5.8 
                 1.1 
               
               
                   
                 2nd 
                 −0.8 
                 2.0 
                 3.6 
                 4.8 
                 0.7 
               
               
                   
                 3rd 
                 −0.8 
                 2.0 
                 3.6 
                 5.3 
                 0.7 
               
               
                   
                 Mean 
                 −0.4 
                 2.0 
                 4.4 
                 5.3 
                 0.8 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 0.8 
                 0.3 
                 0.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 −2.3 
                 2.0 
                   
                 5.2 
                 0.0 
               
               
                   
                 2nd 
                 0.3 
                 2.0 
                 6.1 
                 5.4 
                 1.1 
               
               
                   
                 3rd 
                 −0.3 
                 2.0 
                 4.9 
                 5.6 
                 0.9 
               
               
                   
                 Mean 
                 −0.8 
                 2.0 
                 5.5 
                 5.4 
                 0.7 
               
               
                   
                 STDER 
                 0.8 
                 0.0 
                 0.6 
                 0.1 
                 0.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 −0.3 
                 2.0 
                 4.9 
                 6.2 
                 0.8 
               
               
                   
                 2nd 
                 −2.8 
                 2.0 
                   
                 6.4 
                 0.0 
               
               
                   
                 Mean 
                 −1.5 
                 2.0 
                 4.9 
                 6.3 
                 0.4 
               
               
                   
                 STDER 
                 1.3 
                 0.0 
                 #DIV/0! 
                 0.1 
                 0.4 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 45 
               
             
            
               
                   
               
               
                 Bead name: MMP-9 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                 conc/ 
               
               
                   
                   
                   
                   
                 Concentration 
                 Live cell 
                 live cell 
               
               
                 Sample 
                   
                 MFI 
                 Dilution 
                 (pg/ml) 
                 (10{circumflex over ( )}5/ml) 
                 (pg/10{circumflex over ( )}5) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blank 
                 1st 
                 −0.5 
                 2.0 
                 NaN 
                   
                   
               
               
                   
                 2nd 
                 0.5 
                 2.0 
                 NaN 
               
               
                   
                 Mean 
                 0.0 
                 2.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 #DIV/0! 
                 #DIV/0! 
                 #DIV/0! 
               
               
                 PBMC + 0.5% DMSO 
                 1st 
                 337.5 
                 2.0 
                 1614.0 
                 7.5 
                 215.2 
               
               
                   
                 2nd 
                 556.5 
                 2.0 
                 2589.3 
                 7.4 
                 349.9 
               
               
                   
                 3rd 
                 755.5 
                 2.0 
                 3475.0 
                 6.6 
                 526.5 
               
               
                   
                 Mean 
                 549.8 
                 2.0 
                 2559.4 
                 7.2 
                 363.9 
               
               
                   
                 STDER 
                 120.7 
                 0.0 
                 537.4 
                 0.3 
                 90.1 
               
               
                 PBMC + LPS(5 ug/ml) 
                 1st 
                 29.5 
                 2.0 
                 109.0 
                 6.1 
                 17.9 
               
               
                   
                 2nd 
                 29.5 
                 2.0 
                 109.0 
                 5.8 
                 18.8 
               
               
                   
                 3rd 
                 27.5 
                 2.0 
                 94.9 
                 5.5 
                 17.3 
               
               
                   
                 Mean 
                 28.8 
                 2.0 
                 104.3 
                 5.8 
                 18.0 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 4.7 
                 0.2 
                 0.4 
               
               
                 PBMC + YY101 0.01 uM 
                 1st 
                 1408.5 
                 2.0 
                 6464.0 
                 5.2 
                 1243.1 
               
               
                   
                 2nd 
                 1612.0 
                 2.0 
                 7431.3 
                 4.8 
                 1548.2 
               
               
                   
                 Mean 
                 1510.3 
                 2.0 
                 6947.7 
                 5.0 
                 1395.6 
               
               
                   
                 STDER 
                 101.8 
                 0.0 
                 483.7 
                 0.2 
                 152.6 
               
               
                 PBMC + YY101 0.1 uM 
                 1st 
                 1206.5 
                 2.0 
                 5521.9 
                 4.4 
                 1255.0 
               
               
                   
                 2nd 
                 1844.5 
                 2.0 
                 8560.2 
                 5.8 
                 1475.9 
               
               
                   
                 Mean 
                 1525.5 
                 2.0 
                 7041.0 
                 5.1 
                 1365.4 
               
               
                   
                 STDER 
                 319.0 
                 0.0 
                 1519.1 
                 0.7 
                 110.5 
               
               
                 PBMC + YY101 1 uM 
                 1st 
                 1451.5 
                 2.0 
                 6666.8 
                 5.5 
                 1212.2 
               
               
                   
                 2nd 
                 1598.5 
                 2.0 
                 7366.6 
                 5.5 
                 1339.4 
               
               
                   
                 Mean 
                 1525.0 
                 2.0 
                 7016.7 
                 5.5 
                 1275.8 
               
               
                   
                 STDER 
                 73.5 
                 0.0 
                 349.9 
                 0.0 
                 63.6 
               
               
                 PBMC + YY101 10 uM 
                 1st 
                 2812.5 
                 2.0 
                 13551.9 
                 6.4 
                 2117.5 
               
               
                   
                 2nd 
                 1599.0 
                 2.0 
                 7369.0 
                 6.1 
                 1208.0 
               
               
                   
                 Mean 
                 2205.8 
                 2.0 
                 10460.4 
                 6.3 
                 1662.8 
               
               
                   
                 STDER 
                 606.8 
                 0.0 
                 3091.5 
                 0.2 
                 454.7 
               
               
                 PBMC + YY101 100 uM 
                 1st 
                 1309.5 
                 2.0 
                 6000.1 
                 6.4 
                 937.5 
               
               
                   
                 2nd 
                 1013.0 
                 2.0 
                 4635.0 
                 7.0 
                 662.1 
               
               
                   
                 Mean 
                 1161.3 
                 2.0 
                 5317.6 
                 6.7 
                 799.8 
               
               
                   
                 STDER 
                 148.3 
                 0.0 
                 682.5 
                 0.3 
                 137.7 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.001 uM 
                 1st 
                 37.5 
                 2.0 
                 161.5 
                 6.2 
                 26.1 
               
               
                   
                 2nd 
                 38.5 
                 2.0 
                 167.8 
                 7.6 
                 22.1 
               
               
                   
                 Mean 
                 38.0 
                 2.0 
                 164.7 
                 6.9 
                 24.1 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 3.1 
                 0.7 
                 2.0 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.01 uM 
                 1st 
                 34.5 
                 2.0 
                 142.4 
                 5.2 
                 27.4 
               
               
                   
                 2nd 
                 42.0 
                 2.0 
                 189.4 
                 5.8 
                 32.7 
               
               
                   
                 Mean 
                 38.3 
                 2.0 
                 165.9 
                 5.5 
                 30.0 
               
               
                   
                 STDER 
                 3.8 
                 0.0 
                 23.5 
                 0.3 
                 2.6 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 0.1 uM 
                 1st 
                 28.5 
                 2.0 
                 102.0 
                 6.6 
                 15.5 
               
               
                   
                 2nd 
                 28.5 
                 2.0 
                 102.0 
                 5.7 
                 17.9 
               
               
                   
                 Mean 
                 28.5 
                 2.0 
                 102.0 
                 6.2 
                 16.7 
               
               
                   
                 STDER 
                 0.0 
                 0.0 
                 0.0 
                 0.5 
                 1.2 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 1 uM 
                 1st 
                 21.5 
                 2.0 
                 48.9 
                 4.8 
                 10.2 
               
               
                   
                 2nd 
                 30.5 
                 2.0 
                 115.8 
                 4.4 
                 26.3 
               
               
                   
                 Mean 
                 26.0 
                 2.0 
                 82.3 
                 4.6 
                 18.3 
               
               
                   
                 STDER 
                 4.5 
                 0.0 
                 33.5 
                 0.2 
                 8.1 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 10 uM 
                 1st 
                 20.5 
                 2.0 
                 40.2 
                 5.8 
                 6.9 
               
               
                   
                 2nd 
                 21.5 
                 2.0 
                 48.9 
                 6.4 
                 7.6 
               
               
                   
                 Mean 
                 21.0 
                 2.0 
                 44.5 
                 6.1 
                 7.3 
               
               
                   
                 STDER 
                 0.5 
                 0.0 
                 4.3 
                 0.3 
                 0.4 
               
               
                 PBMC + LPS(5 ug/ml) + Xiidra 100 uM 
                 1st 
                 24.0 
                 2.0 
                 69.0 
                 7.1 
                 9.7 
               
               
                   
                 2nd 
                 24.5 
                 2.0 
                 72.8 
                 5.8 
                 12.6 
               
               
                   
                 Mean 
                 24.3 
                 2.0 
                 70.9 
                 6.5 
                 11.1 
               
               
                   
                 STDER 
                 0.3 
                 0.0 
                 1.9 
                 0.7 
                 1.4 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.001 uM 
                 1st 
                 36.0 
                 2.0 
                 152.0 
                 5.7 
                 26.7 
               
               
                   
                 2nd 
                 36.5 
                 2.0 
                 155.2 
                 7.1 
                 21.9 
               
               
                   
                 3rd 
                 50.5 
                 2.0 
                 239.8 
                 6.6 
                 36.3 
               
               
                   
                 Mean 
                 41.0 
                 2.0 
                 182.3 
                 6.5 
                 28.3 
               
               
                   
                 STDER 
                 4.8 
                 0.0 
                 28.7 
                 0.4 
                 4.3 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.01 uM 
                 1st 
                 37.5 
                 2.0 
                 161.5 
                 6.4 
                 25.2 
               
               
                   
                 2nd 
                 36.5 
                 2.0 
                 155.2 
                 6.0 
                 25.9 
               
               
                   
                 3rd 
                 33.5 
                 2.0 
                 135.8 
                 6.1 
                 22.3 
               
               
                   
                 Mean 
                 35.8 
                 2.0 
                 150.9 
                 6.2 
                 24.5 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 7.7 
                 0.1 
                 1.1 
               
               
                   
                 1st 
                 28.5 
                 2.0 
                 102.0 
                 6.2 
                 16.5 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 0.1 uM 
                 2nd 
                 31.0 
                 2.0 
                 119.2 
                 5.2 
                 22.9 
               
               
                   
                 3rd 
                 31.5 
                 2.0 
                 122.6 
                 6.2 
                 19.8 
               
               
                   
                 Mean 
                 30.3 
                 2.0 
                 114.6 
                 5.9 
                 19.7 
               
               
                   
                 STDER 
                 0.9 
                 0.0 
                 6.4 
                 0.3 
                 1.9 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 1 uM 
                 1st 
                 36.5 
                 2.0 
                 155.2 
                 6.3 
                 24.6 
               
               
                   
                 2nd 
                 37.5 
                 2.0 
                 161.5 
                 7.1 
                 22.8 
               
               
                   
                 3rd 
                 41.5 
                 2.0 
                 186.3 
                 6.2 
                 30.1 
               
               
                   
                 Mean 
                 38.5 
                 2.0 
                 167.7 
                 6.5 
                 25.8 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 9.5 
                 0.3 
                 2.2 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 10 uM 
                 1st 
                 37.5 
                 2.0 
                 161.5 
                 6.0 
                 26.9 
               
               
                   
                 2nd 
                 38.5 
                 2.0 
                 167.8 
                 6.4 
                 26.2 
               
               
                   
                 3rd 
                 34.5 
                 2.0 
                 142.4 
                 7.6 
                 18.7 
               
               
                   
                 Mean 
                 36.8 
                 2.0 
                 157.2 
                 6.7 
                 24.0 
               
               
                   
                 STDER 
                 1.2 
                 0.0 
                 7.7 
                 0.5 
                 2.6 
               
               
                 PBMC + LPS(5 ug/ml) + YY101 100 uM 
                 1st 
                 28.0 
                 2.0 
                 98.5 
                 6.3 
                 15.6 
               
               
                   
                 2nd 
                 28.5 
                 2.0 
                 102.0 
                 6.2 
                 16.5 
               
               
                   
                 3rd 
                 26.5 
                 2.0 
                 87.7 
                 5.0 
                 17.5 
               
               
                   
                 Mean 
                 27.7 
                 2.0 
                 96.1 
                 5.8 
                 16.5 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 4.3 
                 0.4 
                 0.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.001 uM 
                 1st 
                 48.5 
                 2.0 
                 228.2 
                 4.5 
                 50.7 
               
               
                   
                 2nd 
                 41.5 
                 2.0 
                 186.3 
                 5.8 
                 32.1 
               
               
                   
                 3rd 
                 44.5 
                 2.0 
                 204.5 
                 5.8 
                 35.3 
               
               
                   
                 Mean 
                 44.8 
                 2.0 
                 206.3 
                 5.4 
                 39.4 
               
               
                   
                 STDER 
                 2.0 
                 0.0 
                 12.1 
                 0.4 
                 5.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.01 uM 
                 1st 
                 39.5 
                 2.0 
                 174.0 
                 6.3 
                 27.6 
               
               
                   
                 2nd 
                 39.5 
                 2.0 
                 174.0 
                 6.2 
                 28.1 
               
               
                   
                 3rd 
                 42.5 
                 2.0 
                 192.4 
                 5.1 
                 37.7 
               
               
                   
                 Mean 
                 40.5 
                 2.0 
                 180.2 
                 5.9 
                 31.1 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 6.1 
                 0.4 
                 3.3 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 0.1 uM 
                 1st 
                 42.5 
                 2.0 
                 192.4 
                 6.6 
                 29.2 
               
               
                   
                 2nd 
                 39.5 
                 2.0 
                 174.0 
                 5.0 
                 34.8 
               
               
                   
                 3rd 
                 39.5 
                 2.0 
                 174.0 
                 5.7 
                 30.5 
               
               
                   
                 Mean 
                 40.5 
                 2.0 
                 180.2 
                 5.8 
                 31.5 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 6.1 
                 0.5 
                 1.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 1 uM 
                 1st 
                 42.5 
                 2.0 
                 192.4 
                 5.5 
                 35.0 
               
               
                   
                 2nd 
                 40.5 
                 2.0 
                 180.2 
                 4.8 
                 37.5 
               
               
                   
                 3rd 
                 42.5 
                 2.0 
                 192.4 
                 4.8 
                 40.1 
               
               
                   
                 Mean 
                 41.8 
                 2.0 
                 188.3 
                 5.0 
                 37.5 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 4.1 
                 0.2 
                 1.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 10 uM 
                 1st 
                 41.5 
                 2.0 
                 186.3 
                 5.4 
                 34.5 
               
               
                   
                 2nd 
                 43.5 
                 2.0 
                 198.5 
                 5.8 
                 34.2 
               
               
                   
                 3rd 
                 41.5 
                 2.0 
                 186.3 
                 4.7 
                 39.6 
               
               
                   
                 Mean 
                 42.2 
                 2.0 
                 190.4 
                 5.3 
                 36.1 
               
               
                   
                 STDER 
                 0.7 
                 0.0 
                 4.0 
                 0.3 
                 1.8 
               
               
                 PBMC + LPS(5 ug/ml) + YDE011 100 uM 
                 1st 
                 38.5 
                 2.0 
                 167.8 
                 6.1 
                 27.5 
               
               
                   
                 2nd 
                 39.5 
                 2.0 
                 174.0 
                 6.1 
                 28.5 
               
               
                   
                 3rd 
                 37.5 
                 2.0 
                 161.5 
                 6.0 
                 26.9 
               
               
                   
                 Mean 
                 38.5 
                 2.0 
                 167.8 
                 6.1 
                 27.7 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 3.6 
                 0.0 
                 0.5 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.001 uM 
                 1st 
                 39.0 
                 2.0 
                 170.9 
                 4.4 
                 38.8 
               
               
                   
                 2nd 
                 49.5 
                 2.0 
                 234.0 
                 6.0 
                 39.0 
               
               
                   
                 3rd 
                 54.5 
                 2.0 
                 262.8 
                 4.6 
                 57.1 
               
               
                   
                 Mean 
                 47.7 
                 2.0 
                 222.6 
                 5.0 
                 45.0 
               
               
                   
                 STDER 
                 4.6 
                 0.0 
                 27.1 
                 0.5 
                 6.1 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.01 uM 
                 1st 
                 40.5 
                 2.0 
                 180.2 
                 4.5 
                 40.0 
               
               
                   
                 2nd 
                 41.5 
                 2.0 
                 186.3 
                 6.6 
                 28.2 
               
               
                   
                 3rd 
                 42.5 
                 2.0 
                 192.4 
                 5.0 
                 38.5 
               
               
                   
                 Mean 
                 41.5 
                 2.0 
                 186.3 
                 5.4 
                 35.6 
               
               
                   
                 STDER 
                 0.6 
                 0.0 
                 3.5 
                 0.6 
                 3.7 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 0.1 uM 
                 1st 
                 42.5 
                 2.0 
                 192.4 
                 5.0 
                 38.5 
               
               
                   
                 2nd 
                 36.5 
                 2.0 
                 155.2 
                 5.6 
                 27.7 
               
               
                   
                 3rd 
                 39.5 
                 2.0 
                 174.0 
                 5.6 
                 31.1 
               
               
                   
                 Mean 
                 39.5 
                 2.0 
                 173.9 
                 5.4 
                 32.4 
               
               
                   
                 STDER 
                 1.7 
                 0.0 
                 10.7 
                 0.2 
                 3.2 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 1 uM 
                 1st 
                 43.5 
                 2.0 
                 198.5 
                 5.8 
                 34.2 
               
               
                   
                 2nd 
                 36.5 
                 2.0 
                 155.2 
                 4.8 
                 32.3 
               
               
                   
                 3rd 
                 40.5 
                 2.0 
                 180.2 
                 5.3 
                 34.0 
               
               
                   
                 Mean 
                 40.2 
                 2.0 
                 178.0 
                 5.3 
                 33.5 
               
               
                   
                 STDER 
                 2.0 
                 0.0 
                 12.5 
                 0.3 
                 0.6 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 10 uM 
                 1st 
                 43.5 
                 2.0 
                 198.5 
                 5.2 
                 38.2 
               
               
                   
                 2nd 
                 42.5 
                 2.0 
                 192.4 
                 5.4 
                 35.6 
               
               
                   
                 3rd 
                 40.0 
                 2.0 
                 177.1 
                 5.6 
                 31.6 
               
               
                   
                 Mean 
                 42.0 
                 2.0 
                 189.3 
                 5.4 
                 35.1 
               
               
                   
                 STDER 
                 1.0 
                 0.0 
                 6.4 
                 0.1 
                 1.9 
               
               
                 PBMC + LPS(5 ug/ml) + YDE043 100 uM 
                 1st 
                 35.5 
                 2.0 
                 148.8 
                 6.2 
                 24.0 
               
               
                   
                 2nd 
                 32.5 
                 2.0 
                 129.2 
                 6.4 
                 20.2 
               
               
                   
                 Mean 
                 34.0 
                 2.0 
                 139.0 
                 6.3 
                 22.1 
               
               
                   
                 STDER 
                 1.5 
                 0.0 
                 9.8 
                 0.1 
                 1.9 
               
               
                   
               
            
           
         
       
     
     Example 10: Establish Effects of YY-101, YDE-011, YDE-043, YDE-048 and YDE-060 Compounds on 25 Selected Cytokine and Chemokine Release in Tears from Rats with ELGE Dry Eye Condition 
     Objective: 
     
         
         
           
             To establish effects of YY-101, YDE-011, YDE-043, YDE-048 and YDE-060 compounds on 25 selected cytokine and chemokine release in tears from rats with ELGE dry eye condition. 
           
         
       
    
     Materials: 
     
         
         
           
             Rat IL-6 ELISA kit (Thermo fisher, USA) 
             Rat magnetic Luminex assay kit—Procartaplex (Thermo fisher, USA) 
           
         
       
    
     Reference Compound Used: 
     
         
         
           
             Xiidra® (Shire, USA) 
           
         
       
    
     Test Compound 
     
         
         
           
             0.3% YY-101, 0.3% YDE-011, 0.3% YDE-043, 0.3% YDE-048 and 0.3% YDE-060. 
           
         
       
    
     Summary of the Protocol 
     
         
         
           
             1. Rats with ELGE were treated with YY &amp; YDE compound in 5 ul eye drops twice a day for 2 weeks. 
             2. Tear samples were collected using a capillary tube and stored in −80° C. freezer until analysis. 
             3. Tear samples were combined within the group and protein were quantified using the BCA assay. 
             4. Cytokines level is assessed by multiplex assay following manufacturer&#39;s instructions. 
           
         
       
    
     Statistical Analysis 
     
         
         
           
             All values are presented as mean±standard deviation (SD). 
           
         
       
    
     Results 
     The results are shown in  FIGS.  43 - 51    and are presented as concentration (pg/mg protein). All data was normalized to their protein levels. Out of 25 cytokines &amp; chemokines evaluated, 14 of them were within the standard curve range, 3 were below the range, 1 over the range and 7 were below the level of detection &amp; quantification. Since the amount of sample was very small (2-10 uL), each dilution factor was selected in order to generate min. volume for Luminex measurement. 
     
       
         
           
               
               
               
             
               
                 TABLE 46 
               
               
                   
               
               
                 No. 
                 Group 
                 Dilution factor 
               
               
                   
               
             
            
               
                 1 
                 Sham 
                 1/12 
               
               
                 2 
                 ELGE 
                 1/12 
               
               
                 3 
                 Xiidra 
                 1/12 
               
               
                 4 
                 YY-101 
                 1/12 
               
               
                 5 
                 YDE-011 
                 1/14/75 
               
               
                 6 
                 YDE-043 
                 1/12 
               
               
                 S 
                 YDE-048 
                 1/19/33 
               
               
                 8 
                 YDE-060 
                 1/12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 47 
               
               
                   
               
               
                 No. 
                 Cytokine 
                 Result 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 Eotaxin 
                 Within standard curve range 
               
               
                 2 
                 Gro-α_KC 
                 Within standard curve range 
               
               
                 3 
                 IL-17A 
                 Within standard curve range 
               
               
                 4 
                 IL-1 13 
                 Within standard curve range 
               
               
                 5 
                 IL-21 
                 Within standard curve range 
               
               
                 6 
                 IL-4 
                 Within standard curve range 
               
               
                 7 
                 MCP-1 
                 Within standard curve range 
               
               
                 8 
                 MCP-3 
                 Within standard curve range 
               
               
                 9 
                 sVCAM-1 
                 Within standard curve range 
               
               
                 10 
                 TNF-α 
                 Within standard curve range 
               
               
                 11 
                 IL-12p70 
                 Within standard curve range 
               
               
                 12 
                 IL-1 α 
                 Within standard curve range 
               
               
                 13 
                 IP-10 
                 Within standard curve range 
               
               
                 14 
                 VEGF-A 
                 Within standard curve range 
               
               
                 15 
                 bNGF 
                 Below standard curve range 
               
               
                 16 
                 Leptin 
                 Below standard curve range 
               
               
                 17 
                 RANTES 
                 Below standard curve range 
               
               
                 18 
                 CRP 
                 OLQ 
               
               
                 19 
                 TGF-13 
                 BLQ 
               
               
                 20 
                 IFN-y 
                 BLQ 
               
               
                 21 
                 IL-10 
                 BLQ 
               
               
                 22 
                 IL-2 
                 BLQ 
               
               
                 23 
                 IL-6 
                 BLQ 
               
               
                 24 
                 MIP-1 α 
                 BLQ 
               
               
                 25 
                 sRANKL 
                 BLQ 
               
               
                   
               
               
                 Key: 
               
               
                 OLQ = Over the level of quantification 
               
               
                 BLQ = Below the level of Quantification 
               
            
           
         
       
     
     Summary &amp; Conclusion 
     
         
         
           
             Due to small samples of tears, all individual samples were pooled within the groups, thus, the statistical analysis was not performed. Despite of the small sample amount, most of selected cytokine and chemokine levels in the conjunctival epithelial cells were detected within the normal standard range. 
             As expected, a 2 week ELGE induced dry eye condition in rats led to a significant increase of proinflammatory cytokine/chemokine levels in tears of ELGE animals compared to those in the sham control groups. 
             Both the test compounds and the reference compound, Xiidra, significantly reduced most of Th1, Th2 &amp; Th17 derived cytokines and proinflammatory chemokines, suggesting that improvement of corneal damages in ELGE rat dry condition is, at least partly, due to the effective treatment in reducing the proinflammatory cytokine and chemokines in the eye. 
           
         
       
    
     Example 11: Establish Effects of YDE-048 and YDE-043 Compounds on 25 Selected Cytokine and Chemokine Release from Conjunctival Epithelial Cells (Including Goblet Cells) of Rats with ELGE Dry Eye Condition 
     Objective 
     
         
         
           
             To establish effects of YDE-048 and YDE-043 compounds on 25 selected cytokine and chemokine release from conjunctival epithelial cells (including goblet cells) of rats with ELGE dry eye condition (from NSY0319 study). 
           
         
       
    
     Materials 
     
         
         
           
             Rat IL-6 ELISA kit (Thermo fisher, USA, cat no: BMS625) 
             Rat magnetic Luminex assay kit—Procartaplex (Thermo fisher, USA, cat no: PPX-25-MX9HJJU) 
           
         
       
    
     Test Compound 
     
         
         
           
             0.1%, 0.3%, 1%, 3% of YDE-048 &amp; 1% of YDE-043. 
           
         
       
    
     Statistical Analysis 
     
         
         
           
             All values are presented as mean±standard deviation (SD). 
             All values were statistically analyzed by one-way ANOVA with a Tukey, Dunnett&#39;s post test comparing all the groups. 
           
         
       
    
     Results 
     The results are shown in  FIGS.  52 - 61    and are presented as concentration (pg/mg protein). All data was normalized to their protein levels. Out of 25 cytokines &amp; chemokines evaluated, 14 of them were within the standard curve range, 4 were below the range, 1 over the range and 6 were below the level of detection &amp; quantification. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 48 
               
               
                   
               
               
                   
                   
                 Dilution 
                   
               
               
                 No. 
                 Cytokine 
                 factor 
                 Result 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 1 
                 Gro-α_KC 
                 1:01 
                 Within standard curve range 
               
               
                 2 
                 IL-1 α 
                 1:01 
                 Within standard curve range 
               
               
                 3 
                 IL-1 13 
                 1:01 
                 Within standard curve range 
               
               
                 4 
                 Leptin 
                 1:01 
                 Within standard curve range 
               
               
                 5 
                 IP-10 
                 1:01 
                 Within standard curve range 
               
               
                 6 
                 VEGF-A 
                 1:01 
                 Within standard curve range 
               
               
                 7 
                 Eotaxin 
                 1:01 
                 Within standard curve range 
               
               
                 8 
                 IL-17A 
                 1:01 
                 Within standard curve range 
               
               
                 9 
                 MCP-1 
                 1:01 
                 Within standard curve range 
               
               
                 10 
                 MCP-3 
                 1:01 
                 Within standard curve range 
               
               
                 11 
                 RANTES 
                 1:01 
                 Within standard curve range 
               
               
                 12 
                 sVCAM-1 
                 1:01 
                 Within standard curve range 
               
               
                 13 
                 TGF-13 
                 1:01 
                 Within standard curve range 
               
               
                 14 
                 TNF-α 
                 1:01 
                 Within standard curve range 
               
               
                 15 
                 IL-12p70 
                 1:01 
                 Below standard curve range 
               
               
                 16 
                 IL-21 
                 1:01 
                 Below standard curve range 
               
               
                 17 
                 bNGF 
                 1:01 
                 Below standard curve range 
               
               
                 18 
                 IL-4 
                 1:01 
                 Below standard curve range 
               
               
                 19 
                 CRP 
                 1:01 
                 OLQ 
               
               
                 20 
                 IFN-γ 
                 1:01 
                 BLQ 
               
               
                 21 
                 IL-10 
                 1:01 
                 BLQ 
               
               
                 22 
                 IL-2 
                 1:01 
                 BLQ 
               
               
                 23 
                 IL-6 
                 1:01 
                 BLQ 
               
               
                 24 
                 MIP-1 α 
                 1:01 
                 BLQ 
               
               
                 25 
                 sRANKL 
                 1:01 
                 BLQ 
               
               
                   
               
               
                 Key: 
               
               
                 OLQ = Over the level of quantification 
               
               
                 BLQ = Below the level of Quantification 
               
            
           
         
       
     
     Summary &amp; Conclusion 
     
         
         
           
             Most of selected cytokine and chemokine levels in the conjunctival epithelial cells were detected within the normal standard range. 
             Literature suggests that the dry eye condition leads to increase the level of proinflammatory cytokines (e.g., TNF-alpha, IL-6, IL-17) in the conjunctival epithelial cells dry eye disease. However, a 2 week ELGE induced dry eye condition in rats didn&#39;t elicit proinflammatory cytokine/chemokine induction in conjunctival epithelial cells in ELGE animals compared to those in the sham control groups. 
             None of the compounds significantly affected cytokine/chemokine production, suggesting that the compounds have little or no effect the basal level of proinflammatory cytokine/chemokines (including IL-6 levels) in conjunctival epithelial cells although YDE-048 and YDE-043 were effective in improving corneal damaged induced by ELGE. 
           
         
       
    
     Example 12: Efficacy Evaluation of the Compounds of the Invention in Mouse IBD Model 
     DSS Induced Colitis: 
     Experimental schematic timeline is shown in  FIG.  62   . 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 49 
               
               
                   
               
               
                 Group 
                 mpk 
                 N 
                 Route 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 1 
                 Control (No DSS) 
                   
                 6 + 2 
                 SC 
                 B.I.D 
               
               
                 2 
                 DSS 
                   
                 6 + 2 
                 SC 
               
               
                 3 
                 DSS + Cyclosporin A 
                 20 
                 6 + 2 
                 PO 
               
               
                 4 
                 DSS + YDE-048 
                 10 
                 6 + 2 
                 SC 
               
               
                 5 
                 DSS + YDE-048 
                 100 
                 6 + 2 
                 SC 
               
               
                   
               
               
                 YDE-048 is soluble in saline 
               
            
           
         
       
     
     End-point termination—tissue and handling hours after last dosing, collect terminal blood sample, colon
         Blood chemistry evaluation (i.e., CRP)   Histology and pathology evaluation—H&amp;E   Inflammation and oxidative markers (e.g, TNF-α, nitrotyrosine)       

     
       
         
           
               
             
               
                 TABLE 50 
               
               
                   
               
             
            
               
                 Body weight (BW) loss (%) score: 
               
            
           
           
               
               
            
               
                 0-1% 
                 =0 
               
               
                 1-5% 
                 =1 
               
               
                  5-10% 
                 =2 
               
               
                 10-20% 
                 =3 
               
               
                 20% 
                 =4 
               
            
           
           
               
            
               
                 Stool consistency (SC) score: 
               
            
           
           
               
               
            
               
                 Normal 
                 =0 
               
               
                 Soft, but still formed 
                 =1 
               
               
                 Very soft 
                 =2 
               
               
                 Diarrhea 
                 =4 
               
            
           
           
               
            
               
                 Fecal blood occurrence (FB) score: 
               
            
           
           
               
               
            
               
                 Normal 
                 =0 
               
               
                 Visible traces of blood - slight 
                 =1 
               
               
                 Visible traces of blood - critical 
                 =2 
               
               
                 Gross rectal bleeding 
                 =4 
               
               
                 Disease Activity Index (DAI) 
                 =BW score + SC score + FB score 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 51 
               
               
                   
               
               
                 Group 
                 Mpk 
                 N 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 G1 
                 Control (No DSS) 
                   
                 4 
               
               
                 G2 
                 DSS 
                   
                 8 
               
               
                 G3 
                 DSS + Cyclosporin A 
                 20 
                 8 
               
               
                 G4 
                 DSS + YDE-048 
                 10 
                 5 
               
               
                 G5 
                 DSS + YDE-048 
                 100 
                 8 
               
               
                   
               
            
           
         
       
     
     DSS Induced Colitis 
     
         
         
           
             Histology pathology evaluation—H&amp;E 
             Inflammation and oxidative markers—TNF-α, Nitrotyrosine
           TNF-α: 3 image (different 3 layer of on direction) per sample (area: Intestinal villus)   Nitrotyrosine: 2 image (including all layer in Right, Left) per sample   
         
           
         
       
    
     Summary 
     
         
         
           
             3% DSS for five days induced body weight loss, increased disease activity index (DSI) in mice 
             Increased disease index includes body weight loss and increased appearance of loose stools and fecal blood 
             A positive reference compound, cyclosporin A, significantly reduced DSS-induced body weight and increased DSI. 
             YDE-048 at 100 mg/kg significantly reduced DSS-induced increased DSI. In particular, YDE-048 reduced DSS-induced occurrence of loose stools and fecal blood. 
             The level of C-reactive protein (pro-inflammation marker) is highly elevated in plasma of mice with IBD, and YDE-048 partially reduced the level. 
             Future studies will focus on increased sample size, higher dose (e.g., 300 mg/kg) and/or alternative drug delivery (Alzet pump or Matrigel)
 
Immunohistological evaluation on intestines show that TNF-α level is significantly elevated in mice with IBD, and YDE-048 significantly reduced both TNF-α (pro-inflammatory cytokine) and nitrotyrosine (a marker for protein oxidation) levels.
 
The data and results are shown in  FIGS.  62 - 65    and  FIGS.  77 - 79   .
 
           
         
       
    
     Example 13: Mouse Rheumatoid Arthritis Model 
     Summary: 
     
         
         
           
             CII injection led to marked induction of arthritis-like symptoms in mice as early as one day after a CII booster injection and continued to escalated symptoms up to 3 weeks. 
             A reference compound, dexamethasone, strongly reduced inflammation and clinical scores in hind paws although it also lead to a decease in body weight in mice. 
             YDE-048 reduced the clinical symptoms and scores in the first week (an early phase of inflammation) 
             Recommend to repeat the study with increased sample size, higher dose (e.g., 300 mg/kg) and/or alternative drug delivery (Alzet pump or Matrigel)
 
The data and results are shown in  FIGS.  66 - 68   .
 
           
         
       
    
     Example 14: Mouse Osteoporosis Model 
     OVX induced Osteoporosis disease model in C57bL6 mouse 
     Experiment schematic timeline is shown in  FIG.  69   . 
     Study scheme and group information:
         12 week old C57bl6/J Female and generate OVX-induced osteoporosis model   Study Length: 9 Weeks (1 week model generation+8 weeks of Test article dosing)
           Acclamation: 1 week   Dosing: B.I.D/8 weeks   Body Weight and General observation: 8 weeks   
           Report preparation: 7 days   Formulation and test article handling and storage (according to client MSDS)
           Body weight measurement (weekly); uterus weight at the end of experiment   End-point termination:
               Lumbar vertebrae bone strength test   Femurs fixed in 4% paraformaldehyde for μ-CT (e.g., bone mineral density, bone structure, bone quality and volume)   Fixed femurs decalcified in 12% EDTA2Na at RT for approximately 3 weeks and embedded in paraffin. Their slices used for TRAP staining, H&amp;E (hematoxylin and eosin) staining, and immunohistochemical staining.   Body weight   
               Total N=28
               4 groups, n=7 in each group, total 28 mice:   Vehicle, Test article 1, Test article 2   WTN=7   Osteoporosis model N=21   
               
               

     End point sampling:
         body weight   sampling time after 1 hr compound injection   serum   saline perfusion   uterus-&gt;weight, gross   femur (L/R)
           L—in 4% PFA for u-CT (get a sample back)   R—in 10% NBF for staining   
           Lumbar vertebrae in PBS for bone test       

     
       
         
           
               
               
               
               
               
             
               
                 TABLE 52 
               
               
                   
               
               
                 Group 
                 mpk 
                 N 
                 Route 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 1 
                 WT 
                   
                 7 
                 S.C 
                 B.I.D 
               
               
                 2 
                 OVX + Vehicle 
                   
                 7 
                 S.C 
               
               
                 3 
                 OVX + YDE-048 
                 10 
                 7 
                 S.C 
               
               
                 4 
                 OVX + YDE-048 
                 100 
                 7 
                 S.C 
               
               
                   
               
               
                 YDE-048 is soluble in saline 
               
            
           
         
       
     
     Summary 
     
         
         
           
             Ovariectomy (OVX) led to a body weight increase, uterus weight loss in female C57BL/6 mice. 
             Micro-CT scan showed OVX also led to trabecular bone density and connectivity loss in femur with little loss in cortical bone loss in 2 months. 
             OVX didn&#39;t affect lumbar vertebrae bone strength within 2 months. 
             YDE-048 significantly protected trabecular bone loss (both density and volume) in a dose-dependent manner 
             Recommend to repeat the study with increased sample size, higher dose (e.g., 300 mg/kg) and/or alternative drug delivery (Alzet pump or Matrigel)
 
The data and results are shown in  FIGS.  69 - 76   .
 
           
         
       
    
     INCORPORATION BY REFERENCE 
     All publications and patents mentioned herein are hereby incorporated by reference in their entirety as if each individual publication or patent was specifically and individually indicated to be incorporated by reference. In case of conflict, the present application, including any definitions herein, will control. 
     EQUIVALENTS 
     While specific embodiments of the subject invention have been discussed, the above specification is illustrative and not restrictive. Many variations of the invention will become apparent to those skilled in the art upon review of this specification and the claims below. The full scope of the invention should be determined by reference to the claims, along with their full scope of equivalents, and the specification, along with such variations.