Patent Publication Number: US-2022228088-A1

Title: Alpha-amylase variants and polynucleotides encoding same

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is a divisional of U.S. application Ser. No. 16/847,773 filed on Apr. 14, 2020 (now pending) which is a divisional of U.S. application Ser. No. 15/571,219 filed May 9, 2016, now U.S. Pat. No. 10,647,946 which is a 35 U.S.C. 371 national application of PCT/EP2016/060266 filed May 9, 2016, which claims priority or the benefit under 35 U.S.C. 119 of Indian application no. 2335/CHE/2015 filed May 8, 2015, the contents of which are fully incorporated herein by reference. 
    
    
     REFERENCE TO A SEQUENCE LISTING 
     This application contains a Sequence Listing in computer readable form, which is incorporated herein by reference. 
     BACKGROUND OF THE INVENTION 
     Field of the Invention 
     The present invention relates to alpha-amylase variants, polynucleotides encoding the variants, methods of producing the variants, and methods of using the variants. 
     Description of the Related Art 
     Alpha-amylases have for many years been used in laundry where is it well-known that alpha-amylases have a beneficial effect in removal of starch containing, or starch-based, stains. 
     WO95/26397 discloses alkaline  Bacillus  amylases having good wash performance measured at temperatures in the range of 30-60° C. 
     WO00/60060 and WO00/60058 discloses further bacterial alpha-amylases having good wash performance. 
     In recent years there has been a desire to reduce the temperature of the laundry in order to reduce the energy consumption. Lowering the temperature in laundry often means that the performance of the detergent composition and the enzyme is reduced and a lower wash performance is therefore obtained at low temperature. It is therefore desired to find new alpha-amylases having good wash performance at low temperature. Accordingly, it is an object of the present invention to provide alpha-amylases which have good wash performance at low temperature, such as at 15° C. Thus, the present invention provides such further improved alpha-amylase variants with improved properties compared to its parent. 
     SUMMARY OF THE INVENTION 
     The present invention relates to an alpha-amylase variant of a parent polypeptide having alpha-amylase activity, wherein the variant is a fusion polypeptide which has an improved wash performance at low temperature, and wherein said variant has alpha-amylase activity. 
     The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of producing the variants. 
     The present invention also relates to methods of improving wash performance of a parent alpha-amylase. 
     Conventions for Designation of Variants 
     For purposes of the present invention, the mature polypeptide disclosed in SEQ ID NO: 7 is used to determine the corresponding amino acid residue in another alpha-amylase. Alternatively, the mature polypeptide disclosed in SEQ ID NO: 13 and 14 may be used to determine the corresponding amino acid residue in another alpha-amylase. The amino acid sequence of another alpha-amylase is aligned with the mature polypeptide disclosed in SEQ ID NO: 7, and based on the alignment, the amino acid position number corresponding to any amino acid residue in the mature polypeptide disclosed in any of SEQ ID NOs: 13 and 14 is determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970 , J. Mol. Biol.  48: 443-453) as implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000 , Trends Genet.  16: 276-277), preferably version 5.0.0 or later. The parameters used are gap open penalty of 10, gap extension penalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix. 
     Identification of the corresponding amino acid residue in another alpha-amylase can be determined by an alignment of multiple polypeptide sequences using several computer programs including, but not limited to, MUSCLE (multiple sequence comparison by log-expectation; version 3.5 or later; Edgar, 2004 , Nucleic Acids Research  32: 1792-1797), MAFFT (version 6.857 or later; Katoh and Kura, 2002 , Nucleic Acids Research  30: 3059-3066; Katoh et al., 2005 , Nucleic Acids Research  33: 511-518; Katoh and Toh, 2007 , Bioinformatics  23: 372-374; Katoh et al., 2009,  Methods in Molecular Biology  537:_39-64; Katoh and Toh, 2010 , Bioinformatics  26: 1899-1900), and EMBOSS EMMA employing ClustalW (1.83 or later; Thompson et al., 1994 , Nucleic Acids Research  22: 4673-4680), using their respective default parameters. 
     When the other enzyme has diverged from the mature polypeptide of SEQ ID NO: 7 such that traditional sequence-based comparison fails to detect their relationship (Lindahl and Elofsson, 2000 , J. Mol, Bial,  295: 613-615), other pairwise sequence comparison algorithms can be used. Greater sensitivity in sequence-based searching can be attained using search programs that utilize probabilistic representations of polypeptide families (profiles) to search databases: For example, the PSI-BLAST program generates profiles through an iterative database search process and is capable of detecting remote homologs (Atschul et al., 1997 , Nucleic Acids Res.  25: 3389-3402). Even greater sensitivity can be achieved if the family or superfamily for the polypeptide has one or more representatives in the protein structure databases. Programs such as GenTHREADER (Jones, 1999 , J. Mol. Biol.  287: 797-815; McGuffin and Jones, 2003 , Bioinformatics  19: 874-881) utilize information from a variety of sources (PSI-BLAST, secondary structure prediction, structural alignment profiles, and solvation potentials) as input to a neural network that predicts the structural fold for a query sequence. Similarly, the method of Gough et al., 2000 , J. Mol. Biol.  313: 903-919, can be used to align a sequence of unknown structure with the superfamily models present in the SCOP database. These alignments can in turn be used to generate homology models for the polypeptide, and such models can be assessed for accuracy using a variety of tools developed for that purpose. 
     For proteins of known structure, several tools and resources are available for retrieving and generating structural alignments. For example the SCOP superfamilies of proteins have been structurally aligned, and those alignments are accessible and downloadable. Two or more protein structures can be aligned using a variety of algorithms such as the distance alignment matrix (Holm and Sander, 1998 . Proteins  33: 88-96) or combinatorial extension (Shindyalov and Bourne, 1998 , Protein Engineering  11: 739-747), and implementation of these algorithms can additionally be utilized to query structure databases with a structure of interest in order to discover possible structural homologs (e.g., Holm and Park, 2000 , Bioinformatics  16: 566-567). 
     In describing the variants of the present invention, the nomenclature described below is adapted for ease of reference. The accepted IUPAC single letter or three letter amino acid abbreviation is employed. 
     Substitutions: For an amino acid substitution, the following nomenclature is used: Original amino acid, position, substituted amino acid. Accordingly, the substitution of threonine at position 226 with alanine is designated as “Thr226Ala” or “T226A”. Multiple mutations are separated by addition marks (“+”), e.g., “Gly205Arg+Ser411Phe” or “G205R+S411F”, representing substitutions at positions 205 and 411 of glycine (G) with arginine (R) and serine (S) with phenylalanine (F), respectively. 
     Deletions: For an amino acid deletion, the following nomenclature is used: Original amino acid, position, *. Accordingly, the deletion of glycine at position 195 Is designated as “Gly195*” or “G195*”. Multiple deletions are separated by addition marks (“+”), e. “Gly195*+Ser411*” or “G195*+S411*” Multiple modifications: Variants comprising multiple modifications are separated by addition marks (“+”), “Arg170Tyr+Gly195Glu” or “R170Y+G195E” representing a substitution of arginine and glycine at positions 170 and 195 with tyrosine and glutamic acid, respectively. Different modifications: Where different modifications can be introduced at a position, the different alterations are separated by a comma, e.g., “Arg170Tyr,Glu” represents a substitution of arginine at position 170 with tyrosine or glutamic acid. Thus, “Tyr167Gly,Ala+Arg170Gly,Ala” designates the following variants:
     “Tyr167Gly+Arg170Gly”, “Tyr167Gly+Arg170Ala” “Tyr167Ala+Arg170Gly”, and “Tyr167Ala+Arg170Ala”.   

    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The present invention relates to a set of sequences. For ease, these are listed below; 
     SEQ ID NO: 1 is the nucleotide sequence of an alpha-amylase (AA110) 
     SEQ ID NO: 2 is the nucleotide sequence of an alpha-amylase originating from  Alicyclobacillus  sp. 
     SEQ ID NO: 3 is the nucleotide sequence of an alpha-amylase originating from  Bacillus amyloliquefaciens.    
     SEQ ID NO: 4 is the amino acid sequence of AAI10 including the signal peptide. 
     SEQ ID NO: 5 is the amino acid sequence of  Alicyclobacillus  sp. including the signal peptide. 
     SEQ ID NO: 6 is the amino acid sequence of  Bacillus amyloliquefaciens  including the signal peptide. 
     SEQ ID NO: 7 is the amino acid sequence of the mature AAI10 SEQ ID NO: 8 is the amino acid sequence of the mature  Alicyclobacillus  sp. 
     SEQ ID NO: 9 is the amino acid sequence of the mature  Bacillus amyloliquefaciens    
     SEQ ID NO: 10 is the amino acid sequence corresponding the A and B domains of AAI10. 
     SEQ ID NO: 11 is the amino acid sequence corresponding to the C domain of  Alicyclobacillus  sp. 
     SEQ ID NO: 12 is the amino acid sequence responding to the C domain of  Bacillus amyloliquefaciens    
     SEQ ID NO: 13 is the amino acid sequence of the fusion polypeptide consisting of the A and B domain of AAI10 and the C domain of  Alicyclobacillus  sp. 
     SEQ ID NO: 14 is the amino acid sequence of the fusion polypeptide consisting of the A and the B domains of AAI10 and the C domain of  Bacillus amyloliquefaciens    
     SEQ ID NO: 15 is the amino acid sequence of the mature AA110 alpha-amylase comprising a double deletion at positions corresponding to positions 182 and 183 of SEQ ID NO: 7. 
     Variants of the Invention 
     In one aspect, the present invention relates to variants of a parent polypeptide having alpha-amylase activity. Thus, in particular aspect, the present invention relates to variant of a parent polypeptide having alpha-amylase activity, wherein said variant is a fusion polypeptide which has an improved wash performance at low temperature, and wherein said variant has alpha-amylase activity. 
     It has been shown that variants according to the present invention have improved performance, such as wash performance, at a low temperature, in particular at 15° C., in liquid detergent compositions. 
     The term “variant” as used herein, refers to a polypeptide having alpha-amylase activity comprising an alteration, i.e., a substitution, insertion, and/or deletion, at one or more (e.g., several) positions. A substitution means replacement of the amino acid occupying a position with a different amino acid; a deletion means removal of the amino acid occupying a position; and an insertion means adding an amino acid adjacent to and immediately following the amino acid occupying a position, all as defined above. The variants of the present invention have at least 20%, e.g., at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 100% of the alpha-amylase activity of the polypeptide of SEQ ID NOs: 7, 13, 14, 15, or the mature polypeptide of SEQ ID NO: 4. The variants of the present invention have at least 65%, such as at least 70%, such as at least 75%, such as at least 80%, such as at least 85%, such at least 90%, such as at least 95%, such as at least 97%, such as at least 99%, but less than 100% sequence identity to any one of SEQ ID NOs; 4, 7, 13, 14, or 15 
     The term “parent polypeptide” as used herein, refers to a polypeptide to which an alteration is made to produce enzyme variants. The polypeptide having any of SEQ ID NOs: 4, 7, 13, 14, and 15 may e.g. be a parent for the variants of the present invention. Any polypeptide having at least 65%, such as at least 70%, such as at least 75%, such as at least 80%, such as at least 85%, such at least 90%, such as at least 95%, such as at least 97%, such as at least 99%, sequence identity to any one of SEQ ID NOs: 4, 7, 13, 14, or 15 may also be a parent polypeptide for the variants of the present invention. 
     The parent polypeptide may be a fusion polypeptide or cleavable fusion polypeptide. Such fusion polypeptide may consist of a subsequence of one parent polypeptide and a subsequence of second parent polypeptide. In particular, a fusion polypeptide may consist of an A and B domain of one species of alpha-amylase and a C domain of another species of alpha-amylase, and thereby providing a parent polypeptide to generate a variant of the present invention. Techniques for producing fusion polypeptides are known in the art, and include ligating the coding sequences encoding the polypeptides so that they are in frame and that expression of the fusion polypeptide is under control of the same promoter(s) and terminator. Fusion polypeptides may also be constructed using intein technology in which fusion polypeptides are created post-translationally (Cooper et al., 1993 , EMBO J.  12: 2575-2583; Dawson et al., 1994 , Science  266: 776-779). 
     A fusion polypeptide may further comprise a cleavage site between the two polypeptides. Upon secretion of the fusion protein, the site is cleaved releasing the two polypeptides. Examples of cleavage sites include, but are not limited to, the sites disclosed in Martin et al., 2003 , J. Ind. Microbiol. Biotechnol.  3: 568-576; Svetina et al., 2000 , J. Biotechnol,  76: 245-251; Rasmussen-Wilson et al., 1997 , App. Environ, Microbiol.  63: 3488-3493; Ward et al., 1995 , Biotechnology  13: 498-503; and Contreras et al., 1991, Biotechnology 9; 378-381; Eaton et al., 1986 . Biochemistry  25: 505-512; Collins-Racie et a, 1995 , Biotechnology  13: 982-987; Carter et al., 1989, Proteins: Structure,  Function, and Genetics  6: 240-248; and Stevens, 2003 , Drug Discovery World  4: 35-48. 
     The parent polypeptide may be obtained from microorganisms of any genus. For purposes of the present invention, the term “obtained from” as used herein in connection with a given source shall mean that the parent polypeptide encoded by a polynucleotide is produced by the source or by a strain in which the polynucleotide from the source has been inserted. 
     In some aspects of the present invention, the parent polypeptide is  Bacillus  sp. alpha-amylase, e.g., the alpha-amylase of SEQ ID NOs: 4, the mature polypeptide thereof, ie. SEQ ID NO: 7. In particular aspects, the parent polypeptide is a fusion protein, and originates from both a  Bacillus  sp and a  Alicyclobacilius  sp., e.g. the alpha-amylase of SEQ ID NO: 13. In other aspects, the parent polypeptide is a fusion protein and originates from two different  Bacillus  sps., e.g, resulting in the alpha-amylase of SEQ ID NO: 14. 
     It will be understood that for the aforementioned species, the invention encompasses both the perfect and imperfect states, and other taxonomic equivalents, e.g., anamorphs, regardless of the species name by which they are known. Those skilled in the art will readily recognize the identity of appropriate equivalents. 
     Strains of these species are readily accessible to the public in a number of culture collections, such as the American Type Culture Collection (ATCC), Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ), Centraalbureau Voor Schimmelcultures (CBS), and Agricultural Research Service Patent Culture Collection, Northern Regional Research Center (NRRL). 
     The parent polypeptide may be identified and obtained from other sources including microorganisms isolated from nature (e.g., soil, composts, water, etc.) or DNA samples obtained directly from natural materials (e.g., soil, composts, water, etc.) using the above-mentioned probes. Techniques for isolating microorganisms and DNA directly from natural habitats are well known in the art. A polynucleotide encoding a parent polypeptide may then be obtained by similarly screening a genomic DNA or cDNA library of another microorganism or mixed DNA sample. Once a polynucleotide encoding a parent polypeptide has been detected with the probe(s), the polynucleotide can be isolated or cloned by utilizing techniques that are known to those of ordinary skill in the art (see, e.g., Sambrook et al., 1989, supra). 
     In one embodiment, the parent polypeptide comprises or consists of the amino acid sequence set forth in SEQ ID NO: 7, 13, or 15. In another embodiment, the parent polypeptide comprises or consists of the mature polypeptide of SEQ ID NO: 4. In particular, the parent polypeptide may comprise or consist of amino acids 1 to 485 of SEQ ID NO: 4. 
     In another embodiment, the parent polypeptide is a fragment of the mature polypeptide of SEQ ID NO: 4 containing at least 350, such as at least 390 amino acid residues, e.g., at least 395 and at least 397 amino acid residues of SEQ ID NO: 4. 
     In another embodiment, the parent polypeptide is an allelic variant of the mature polypeptide of SEQ ID NO: 4. 
     The term “sequence identity” as used herein, refers to the relatedness between two amino acid sequences or between two nucleotide sequences. For purposes of the present invention, the sequence identity between two amino acid sequences is determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970 , J. Mol. Biol.  48: 443-453) as implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000 , Trends Genet.  16: 276-277), preferably version 5.0.0 or later. The parameters used are gap open penalty of 10, gap extension penalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix. The output of Needle labeled “longest identity” (obtained using the—nobrief option) is used as the percent identity and is calculated as follows: 
       (Identical Residues×100)/(Length of Alignment Total Number of Gaps in Alignment)
 
     For purposes of the present invention, the sequence identity between two deoxyribonucleotide sequences is determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, supra) as implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, supra), preferably version 5.0.0 or later. The parameters used are gap open penalty of 10, gap extension penalty of 0.5, and the EDNAFULL (EMBOSS version of NCBI NUC4.4) substitution matrix. The output of Needle labeled “longest identity” (obtained using the nobrief option) is used as the percent identity and is calculated as follows: 
       (Identical Deoxyribonucleotides×100)/(Length of Alignment Total Number of Gaps in Alignment)
 
     The term “alpha-amylase activity” as used herein, refers to the activity of alpha-1,4-glucan-4-glucanohydrolases, E.C. 3.2.1.1, which constitute a group of enzymes, catalyzing hydrolysis of starch and other linear and branched 1,4-glucosidic oligo- and polysaccharides. Thus, the term “alpha-amylase” as used herein, refers to an enzyme that has alpha-amylase activity (Enzyme Class; EC 3.2.1.1) that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. The terms “alpha-amylase” and “amylase” may be used interchangeably and constitute the same meaning and purpose within the scope of the present invention. For purposes of the present invention, alpha-amylase activity is determined according to the procedure described in the Examples. In one embodiment, the variants of the present invention have at least 20%, e.g.; at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 100% of the alpha-amylase activity of the polypeptide of SEQ ID NOs: 7, 13, 14, or 15; or the mature polypeptide of SEQ ID NO: 4. 
     The term “fusion polypeptide” as used herein, refers to a polypeptide which comprises amino acid sequences originating from more than one, such as two, three, or four, species. Such a fusion polypeptide may have been generated by molecular techniques well-Known to the skilled person. A fusion polypeptide of the present invention has alpha-amylase activity. In particular, a fusion polypeptide of the present invention, comprises e.g. an A and B domain of one alpha-amylase and a C domain from another alpha-amylase. 
     The term “wash performance” as used herein, refers to an enzyme&#39;s ability to remove starch or starch-containing stains present on the object to be cleaned during e.g. laundry or hard surface cleaning, such as dish wash. The term “wash performance” includes cleaning in general e.g, hard surface cleaning as in dish wash, but also wash performance on textiles such as laundry, and also industrial and institutional cleaning. The wash performance may be quantified by calculating the so-called Intensity value, and results may be displayed as “Improvement Factor” (IF). Wash performance may be determined as in described in the Examples herein. 
     The term “Intensity value” as used herein, refers to the wash performance measured as the brightness expressed as the intensity of the light reflected from the sample when illuminated with white light. When the sample is stained the intensity of the reflected light is lower, than that of a clean sample. Therefore the intensity of the reflected light can be used to measure wash performance, where a higher intensity value correlates with higher wash performance. 
     Color measurements are made with a professional flatbed scanner (Kodak iQsmart, Kodak) used to capture an image of the washed textile. 
     To extract a value for the light intensity from the scanned images, 24-bit pixel values from the image are converted into values for red, green and blue (RGB). The intensity value (Int) is calculated by adding the RGB values together as vectors and then taking the length of the resulting vector: 
     
       
         
           
             Int 
             = 
             
               
                 
                   r 
                   2 
                 
                 + 
                 
                   g 
                   2 
                 
                 + 
                 
                   b 
                   2 
                 
               
             
           
         
       
     
     The term “improved wash performance” as used herein, refers to an improvement of the wash performance of an alpha-amylase of the present invention relative to the wash performance of the parent polypeptide. Improved wash performance may be measured by comparing of the so-called Intensity value and calculating the Improvement Factor (IF). The improved wash performance is determined according to the section “Wash performance of alpha-amylases using Automatic Mechanical Stress Assay” and using model detergent J at 15° C., 30° C. or 40° C. Other model detergents may be used, such as Model detergent A. 
     Thus, in one embodiment, the improved wash performance is determined by a method comprising the steps of;
         a) washing a fabric stained with starch with an alpha-amylase variant and a parent polypeptide sample added, respectively, to a model detergent composition, such as Model A or Model J;   b) measuring the intensity of light reflected from the sample when illuminated with white light; and   c) optionally, calculating the improvement factor (IF) as the ratio of delta intensity of the alpha-amylase sample over the delta intensity of the parent polypeptide sample.       

     In one embodiment, the improved wash performance is determined by a method comprising the steps of;
         a) washing a fabric stained with starch with an alpha-amylase variant and a parent polypeptide sample added, respectively, to a model detergent composition, such as Model A or Model J for 20 minutes at 15° C., 30° C., and 40° C.;   b) measuring the intensity of light reflected from the sample when illuminated with white light; and   c) optionally, calculating the improvement factor (IF) as the ration of delta intensity of the alpha-amylase sample over the delta intensity of the parent polypeptide sample.       

     The term “low temperature” as used herein, refers to is a temperature of 5-40° C., such as 5-35° C., preferably 5-30° C., more preferably 5-25° C., more preferably 5-20° C., most preferably 5-15° C., and in particular 5-10° C., In a preferred embodiment, “Low temperature” is a temperature of 10-35° C., preferably 10-30° C. more preferably 10-25° C., most preferably 10-20° C., and in particular 10-15° C. Most preferred, low temperature means 15° C. 
     Thus, in one embodiment, the low temperature is 5° C. to 40° C., preferably 10° C. to 30°, event more preferred 15° C. to 20° C. 
     In one embodiment, the improved wash performance has an Improvement Factor (IF) of ≥1 when compared to said parent polypeptide having alpha-amylase activity. 
     The term “Improvement Factor” as used herein, refers to a quantitative way of calculating the improvement of a particular property of a variant according to the present invention. Determination of the Improvement Factor may be according to the following formula: 
     
       
         
           
             
               
                 Intensity 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 value 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 of 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 variant 
               
               - 
               
                 Intensity 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 value 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 of 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 blank 
               
             
             
               
                 Intensity 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 value 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 of 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 parent 
               
               - 
               
                 Intensity 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 value 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 of 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 blank 
               
             
           
         
       
     
     Other formulas may be used to determine the Improvement Factor. The skilled person knows the presently presented formula as well as alternative ways of calculating the Improvement Factor. 
     According to the present invention, a value of 1.0 corresponds to the performance observed for the parent polypeptide. A value above 1.0 indicates an improvement of performance of the variant tested compared to the parent polypeptide. Accordingly, any value of ≥1.0 is indicative for improvement of property, such as performance, of the variant compared to the parent polypeptide. 
     According to the present invention, a variant showing improvement of property under at least one condition tested, is considered a variant having improved property as compared to the parent polypeptide. 
     In one embodiment, said parent polypeptide comprises an A and a B domain comprising the amino acid sequence of SEQ ID NO: 10, or an A and a B domain having at least 80%, such as 85%, such as 90%, such as 95%, such as 97% sequence identity to SEQ ID NO: 10, and a C domain of a low temperature alpha-amylase. 
     The terms “A domain”, “B domain” and “C domain” as used herein, refers to three distinct domains A, B and C, all part of the alpha-amylase structure, see, e.g., Machius et al., 1995 , J. Mol. Biol.  246: 545-559, Thus, an alpha-amylase, such as a parent polypeptide and a variant according to the invention, may comprise both an A, 8, and C domain. The term “domain” means a region of a polypeptide that in itself forms a distinct and independent substructure of the whole molecule. Alpha-amylases consist of a beta/alpha-8 barrel harboring the active site residues, which is denoted the A domain, a rather long loop between the beta-sheet 3 and alpha-helix 3, which is denoted the B domain (together; “A and B domain”), and a C-domain and in some cases also a carbohydrate binding domain (e.g., WO 2005/001064; Machius et al., supra). 
     The domains of an alpha-amylase may be determined by structure analysis such as using crystallographically techniques. An alternative method for determining the domains of an alpha-amylase is by sequence alignment of the amino acid sequence of the alpha-amylase with another alpha-amylase for which the domains have been determined. The sequence that aligns with, e.g., the C-domain sequence in the alpha-amylase for which the C-domain has been determined can be considered the C domain for the given alpha-amylase. 
     The term “A and B domain” as used herein, refers to two domains of an alpha-amylase taken as one unit, whereas the C domain is another unit of the alpha-amylases. Thus, the amimo acid sequence of the “A and B domain” is understood as one sequence or one part of a sequence of an alpha-amylase comprising an “A and B domain” and other domains (such as the C domain). Thus, the term “the A and B domain domain has at least 65% sequence identity to SEQ ID NO: 10” means that the amino acid sequence that form the A and B domain has at least 65% sequence identity to SEQ ID NO: 10. As used herein, the “A and B domain” of a parent polypeptide corresponds to amino acids 1 to 399 of SEQ ID NO: 7, or to amino acids 1 to 397 of SEQ ID NO: 15. The A and B domain of a parent polypeptide may have the amino acid sequence of SEQ ID NO: 10. 
     The term “C domain” as used herein, refers to a domain of an alpha-amylase as one unit. The “C domain” of an alpha-amylase corresponds to amino acids 400 to 485 of SEQ ID NO: 7, or to amino acid 398 to 483 of SEQ ID NO: 15, Thus, the C domain of an alpha amylase may be found by alignment of said alpha-amylase with the polypeptide of SEQ ID NO: 7 or 15. The part of said alpha-amylase that aligns with amino acids 400 to 485 of SEQ ID NO: 7 or to amino acids 398 to 483 of SEQ ID NO: 15 is according to the present invention “the C domain” of the alpha-amylase. The C domain of a parent polypeptide may have the amino acid sequence of SEQ ID NOs: 11 or 12. 
     The term “C domain of a low temperature alpha-amylase” as used herein, refers to the C domain of an alpha-amylase showing particular properties at low temperatures. Non-limiting examples of such low temperature alpha-amylases are those originating from  Alicyclobacillus  sp. as well as from  Bacillus amyloliquefaciens  (BAN). 
     In one embodiment, the parent polypeptide comprises a C domain comprising the amino acid sequence of SEQ ID NOs: 11 or 12, or a C domain having at 80%, such as 85%, such as 90%, such as 95%, such as 97%, sequence identity to SEQ ID NO: 11 or 12. 
     In one embodiment, the parent polypeptide comprises the amino acid sequence of SEQ ID NOs: 13 or 14, or a sequence having at least 80%, such as 85%, such as 90%, such as 95%, such as 97% sequence identity to SEQ ID NOs: 13 or 14. 
     As stated elsewhere herein, the parent polypeptide may be any polypeptide having alpha-amylase activity and at least 65% sequence identity to any one of the amino acid sequences as set forth in SEQ ID NOs: 13 and 14. In certain embodiments, the parent polypeptide comprises the amino acid sequences as set forth in SEQ ID NOs: 10, 11, and 12. 
     In one embodiment, the parent polypeptide a sequence identity to the amino acid sequence set forth in SEQ ID NOs: 13 or 14 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%, and which parent polypeptide has alpha-amylase activity. In one embodiment, the amino acid sequence of the parent polypeptide differs by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the amino acid sequences set forth in SEQ ID NOs: 7, 13, and 14. 
     The present invention provides variants of such parent polypeptides. 
     The variants according to the present invention may advantageously comprise alterations in specific positions of the A and B domain. Thus, in a particular embodiment, the variant comprises amino acid alterations in amino acid positions corresponding the amino acid sequence set forth in SEQ ID NO: 10, or a sequence having at least 65% sequence identity to the amino acid sequence set forth in SEQ ID NO: 10. 
     In particular, the present invention relates to an embodiment, the A and B domains comprise a modification in one or more of the following positions corresponding to positions; H1, T5, G7, Q11, N16, V17, Q32, A37, 140, P45, W48, G50, T51, N54, V56, A60, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, I165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, I193, N195, G196, A204, V206, P211, I214, V215, L217, L219, A225, L228, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, A265, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, N295, Q299, S304, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, and Q395 of SEQ ID NOs: 7 or 10. 
     Such variants have shown to have improved wash performance in at least one assay wherein the temperature under which the variants have been tested was a low temperature, such as 15° C., 30° and 40° C. Specific conditions may be seen in the Examples herein. 
     The term “modification” as used herein, refers to both substitutions and deletions of amino acid within the amino acid sequence of a polypeptide. The terms “alteration” and “modification” may be used interchangeably herein. This should not be understood as any limitation and thus, the terms constitute the same meaning and purpose unless explicitly stated otherwise. 
     The term “corresponding to” as used herein, refers to way of determining the specific amino acid of a sequence wherein reference is made to a specific amino acid sequence. E.g. for the purposes of the present invention, when references are made to specific amino acid positions, the skilled person would be able to align another amino acid sequence to said amino acid sequence that reference has been made to, in order to determine which specific amino acid may be of interest in said another amino acid sequence. Alignment of another amino acid sequence with e.g. the sequence as set forth in SEQ ID NO: 4, 7, or any other sequence listed herein, has been described elsewhere herein. Alternative alignment methods may be used, and are well-known for the skilled person. 
     In a particular embodiment, the variant comprises an alteration in two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, or sixteen positions corresponding to the following positions; H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, A60, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, G196, A204, V206, P211, I214, V215, L217, L219, A225, L228, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, A265, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, S304, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, and Q395 of SEQ ID NOs: 7 or 10. 
     In a particular embodiment, the variant comprises an alteration in the following positions; 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
             
            
               
                 H1 + T5 
                 T40 + L217 
                 N54 + T285 
                 Q125 + G255 
                 G184 + P211 
                 M248 + Q299 
               
               
                 H1 + G7 
                 T40 + L219 
                 N54 + M286 
                 Q125 + Q256 
                 G184 + I214 
                 M248 + S304 
               
               
                 H1 + Q11 
                 T40 + L228 
                 N54 + F289 
                 Q125 + A263 
                 G184 + V215 
                 M248 + R320 
               
               
                 H1 + N16 
                 T40 + L235 
                 N54 + V291 
                 Q125 + V264 
                 G184 + L217 
                 M248 + H321 
               
               
                 H1 + V17 
                 T40 + V238 
                 N54 + Q299 
                 Q125 + A265 
                 G184 + L219 
                 M248 + S323 
               
               
                 H1 + Q32 
                 T40 + M246 
                 N54 + S304 
                 Q125 + Y267 
                 G184 + L228 
                 M248 + H324 
               
               
                 H1 + A37 
                 T40 + M248 
                 N54 + R320 
                 Q125 + N270 
                 G184 + L235 
                 M248 + F328 
               
               
                 H1 + T40 
                 T40 + L250 
                 N54 + H321 
                 Q125 + G273 
                 G184 + V238 
                 M248 + T334 
               
               
                 H1 + P45 
                 T40 + G255 
                 N54 + S323 
                 Q125 + S280 
                 G184 + M246 
                 M248 + D337 
               
               
                 H1 + W48 
                 T40 + Q256 
                 N54 + H324 
                 Q125 + T285 
                 G184 + M248 
                 M248 + Q345 
               
               
                 H1 + G50 
                 T40 + A263 
                 N54 + F328 
                 Q125 + M286 
                 G184 + L250 
                 M248 + G346 
               
               
                 H1 + T51 
                 T40 + V264 
                 N54 + T334 
                 Q125 + F289 
                 G184 + G255 
                 M248 + G348 
               
               
                 H1 + N54 
                 T40 + A265 
                 N54 + D337 
                 Q125 + V291 
                 G184 + Q256 
                 M248 + T355 
               
               
                 H1 + V56 
                 T40 + Y267 
                 N54 + Q345 
                 Q125 + Q299 
                 G184 + A263 
                 M248 + S376 
               
               
                 H1 + A60 
                 T40 + N270 
                 N54 + G346 
                 Q125 + S304 
                 G184 + V264 
                 M248 + D377 
               
               
                 H1 + K72 
                 T40 + G273 
                 N54 + G348 
                 Q125 + R320 
                 G184 + A265 
                 M248 + D379 
               
               
                 H1 + R87 
                 T40 + S280 
                 N54 + T355 
                 Q125 + H321 
                 G184 + Y267 
                 M248 + Y382 
               
               
                 H1 + Q98 
                 T40 + T285 
                 N54 + S376 
                 Q125 + S323 
                 G184 + N270 
                 M248 + S383 
               
               
                 H1 + M105 
                 T40 + M286 
                 N54 + D377 
                 Q125 + H324 
                 G184 + G273 
                 M248 + Q385 
               
               
                 H1 + G109 
                 T40 + F289 
                 N54 + D379 
                 Q125 + F328 
                 G184 + S280 
                 M248 + K391 
               
               
                 H1 + F113 
                 T40 + V291 
                 N54 + Y382 
                 Q125 + T334 
                 G184 + T285 
                 M248 + K393 
               
               
                 H1 + R116 
                 T40 + Q299 
                 N54 + S383 
                 Q125 + D337 
                 G184 + M286 
                 M248 + Q395 
               
               
                 H1 + Q118 
                 T40 + S304 
                 N54 + Q385 
                 Q125 + Q345 
                 G184 + F289 
                 L250 + G255 
               
               
                 H1 + Q125 
                 T40 + R320 
                 N54 + K391 
                 Q125 + G346 
                 G184 + V291 
                 L250 + Q256 
               
               
                 H1 + G133 
                 T40 + H321 
                 N54 + K393 
                 Q125 + G348 
                 G184 + Q299 
                 L250 + A263 
               
               
                 H1 + T134 
                 T40 + S323 
                 N54 + Q395 
                 Q125 + T355 
                 G184 + S304 
                 L250 + V264 
               
               
                 H1 + W140 
                 T40 + H324 
                 V56 + A60 
                 Q125 + S376 
                 G184 + R320 
                 L250 + A265 
               
               
                 H1 + G142 
                 T40 + F328 
                 V56 + K72 
                 Q125 + D377 
                 G184 + H321 
                 L250 + Y267 
               
               
                 H1 + G149 
                 T40 + T334 
                 V56 + R87 
                 Q125 + D379 
                 G184 + S323 
                 L250 + N270 
               
               
                 H1 + T165 
                 T40 + D337 
                 V56 + Q98 
                 Q125 + Y382 
                 G184 + H324 
                 L250 + G273 
               
               
                 H1 + W167 
                 T40 + Q345 
                 V56 + M105 
                 Q125 + S383 
                 G184 + F328 
                 L250 + S280 
               
               
                 H1 + R171 
                 T40 + G346 
                 V56 + G109 
                 Q125 + Q385 
                 G184 + T334 
                 L250 + T285 
               
               
                 H1 + Q172 
                 T40 + G348 
                 V56 + F113 
                 Q125 + K391 
                 G184 + D337 
                 L250 + M286 
               
               
                 H1 + L173 
                 T40 + T355 
                 V56 + R116 
                 Q125 + K393 
                 G184 + Q345 
                 L250 + F289 
               
               
                 H1 + A174 
                 T40 + S376 
                 V56 + Q118 
                 Q125 + Q395 
                 G184 + G346 
                 L250 + V291 
               
               
                 H1 + G184 
                 T40 + D377 
                 V56 + Q125 
                 G133 + T134 
                 G184 + G348 
                 L250 + Q299 
               
               
                 H1 + T193 
                 T40 + D379 
                 V56 + G133 
                 G133 + W140 
                 G184 + T355 
                 L250 + S304 
               
               
                 H1 + N195 
                 T40 + Y382 
                 V56 + T134 
                 G133 + G142 
                 G184 + S376 
                 L250 + R320 
               
               
                 H1 + G196 
                 T40 + S383 
                 V56 + W140 
                 G133 + G149 
                 G184 + D377 
                 L250 + H321 
               
               
                 H1 + A204 
                 T40 + Q385 
                 V56 + G142 
                 G133 + T165 
                 G184 + D379 
                 L250 + S323 
               
               
                 H1 + V206 
                 T40 + K391 
                 V56 + G149 
                 G133 + W167 
                 G184 + Y382 
                 L250 + H324 
               
               
                 H1 + P211 
                 T40 + K393 
                 V56 + T165 
                 G133 + R171 
                 G184 + S383 
                 L250 + F328 
               
               
                 H1 + I214 
                 T40 + Q395 
                 V56 + W167 
                 G133 + Q172 
                 G184 + Q385 
                 L250 + T334 
               
               
                 H1 + V215 
                 P45 + W48 
                 V56 + R171 
                 G133 + L173 
                 G184 + K391 
                 L250 + D337 
               
               
                 H1 + L217 
                 P45 + G50 
                 V56 + Q172 
                 G133 + A174 
                 G184 + K393 
                 L250 + Q345 
               
               
                 H1 + L219 
                 P45 + T51 
                 V56 + L173 
                 G133 + G184 
                 G184 + Q395 
                 L250 + G346 
               
               
                 H1 + L228 
                 P45 + N54 
                 V56 + A174 
                 G133 + T193 
                 T193 + N195 
                 L250 + G348 
               
               
                 H1 + L235 
                 P45 + V56 
                 V56 + G184 
                 G133 + N195 
                 T193 + G196 
                 L250 + T355 
               
               
                 H1 + V238 
                 P45 + A60 
                 V56 + T193 
                 G133 + G196 
                 T193 + A204 
                 L250 + S376 
               
               
                 H1 + M246 
                 P45 + K72 
                 V56 + N195 
                 G133 + A204 
                 T193 + V206 
                 L250 + D377 
               
               
                 H1 + M248 
                 P45 + R87 
                 V56 + G196 
                 G133 + V206 
                 T193 + P211 
                 L250 + D379 
               
               
                 H1 + L250 
                 P45 + Q98 
                 V56 + A204 
                 G133 + P211 
                 T193 + I214 
                 L250 + Y382 
               
               
                 H1 + G255 
                 P45 + M105 
                 V56 + V206 
                 G133 + I214 
                 T193 + V215 
                 L250 + S383 
               
               
                 H1 + Q256 
                 P45 + G109 
                 V56 + P211 
                 G133 + V215 
                 T193 + L217 
                 L250 + Q385 
               
               
                 H1 + A263 
                 P45 + F113 
                 V56 + I214 
                 G133 + L217 
                 T193 + L219 
                 L250 + K391 
               
               
                 H1 + V264 
                 P45 + R116 
                 V56 + V215 
                 G133 + L219 
                 T193 + L228 
                 L250 + K393 
               
               
                 H1 + A265 
                 P45 + Q118 
                 V56 + L217 
                 G133 + L228 
                 T193 + L235 
                 L250 + Q395 
               
               
                 H1 + Y267 
                 P45 + Q125 
                 V56 + L219 
                 G133 + L235 
                 T193 + V238 
                 G255 + Q256 
               
               
                 H1 + N270 
                 P45 + G133 
                 V56 + L228 
                 G133 + V238 
                 T193 + M246 
                 G255 + A263 
               
               
                 H1 + G273 
                 P45 + T134 
                 V56 + L235 
                 G133 + M246 
                 T193 + M248 
                 G255 + V264 
               
               
                 H1 + S280 
                 P45 + W140 
                 V56 + V238 
                 G133 + M248 
                 T193 + L250 
                 G255 + A265 
               
               
                 H1 + T285 
                 P45 + G142 
                 V56 + M246 
                 G133 + L250 
                 T193 + G255 
                 G255 + Y267 
               
               
                 H1 + M286 
                 P45 + G149 
                 V56 + M248 
                 G133 + G255 
                 T193 + Q256 
                 G255 + N270 
               
               
                 H1 + F289 
                 P45 + T165 
                 V56 + L250 
                 G133 + Q256 
                 T193 + A263 
                 G255 + G273 
               
               
                 H1 + V291 
                 P45 + W167 
                 V56 + G255 
                 G133 + A263 
                 T193 + V264 
                 G255 + S280 
               
               
                 H1 + Q299 
                 P45 + R171 
                 V56 + Q256 
                 G133 + V264 
                 T193 + A265 
                 G255 + T285 
               
               
                 H1 + S304 
                 P45 + Q172 
                 V56 + A263 
                 G133 + A265 
                 T193 + Y267 
                 G255 + M286 
               
               
                 H1 + R320 
                 P45 + L173 
                 V56 + V264 
                 G133 + Y267 
                 T193 + N270 
                 G255 + F289 
               
               
                 H1 + H321 
                 P45 + A174 
                 V56 + A265 
                 G133 + N270 
                 T193 + G273 
                 G255 + V291 
               
               
                 H1 + S323 
                 P45 + G184 
                 V56 + Y267 
                 G133 + G273 
                 T193 + S280 
                 G255 + Q299 
               
               
                 H1 + H324 
                 P45 + T193 
                 V56 + N270 
                 G133 + S280 
                 T193 + T285 
                 G255 + S304 
               
               
                 H1 + F328 
                 P45 + N195 
                 V56 + G273 
                 G133 + T285 
                 T193 + M286 
                 G255 + R320 
               
               
                 H1 + T334 
                 P45 + G196 
                 V56 + S280 
                 G133 + M286 
                 T193 + F289 
                 G255 + H321 
               
               
                 H1 + D337 
                 P45 + A204 
                 V56 + T285 
                 G133 + F289 
                 T193 + V291 
                 G255 + S323 
               
               
                 H1 + Q345 
                 P45 + V206 
                 V56 + M286 
                 G133 + V291 
                 T193 + Q299 
                 G255 + H324 
               
               
                 H1 + G346 
                 P45 + P211 
                 V56 + F289 
                 G133 + Q299 
                 T193 + S304 
                 G255 + F328 
               
               
                 H1 + G348 
                 P45 + I214 
                 V56 + V291 
                 G133 + S304 
                 T193 + R320 
                 G255 + T334 
               
               
                 H1 + T355 
                 P45 + V215 
                 V56 + Q299 
                 G133 + R320 
                 T193 + H321 
                 G255 + D337 
               
               
                 H1 + S376 
                 P45 + L217 
                 V56 + S304 
                 G133 + H321 
                 T193 + S323 
                 G255 + Q345 
               
               
                 H1 + D377 
                 P45 + L219 
                 V56 + R320 
                 G133 + S323 
                 T193 + H324 
                 G255 + G346 
               
               
                 H1 + D379 
                 P45 + L228 
                 V56 + H321 
                 G133 + H324 
                 T193 + F328 
                 G255 + G348 
               
               
                 H1 + Y382 
                 P45 + L235 
                 V56 + S323 
                 G133 + F328 
                 T193 + T334 
                 G255 + T355 
               
               
                 H1 + S383 
                 P45 + V238 
                 V56 + H324 
                 G133 + T334 
                 T193 + D337 
                 G255 + S376 
               
               
                 H1 + Q385 
                 P45 + M246 
                 V56 + F328 
                 G133 + D337 
                 T193 + Q345 
                 G255 + D377 
               
               
                 H1 + K391 
                 P45 + M248 
                 V56 + T334 
                 G133 + Q345 
                 T193 + G346 
                 G255 + D379 
               
               
                 H1 + K393 
                 P45 + L250 
                 V56 + D337 
                 G133 + G346 
                 T193 + G348 
                 G255 + Y382 
               
               
                 H1 + Q395 
                 P45 + G255 
                 V56 + Q345 
                 G133 + G348 
                 T193 + T355 
                 G255 + S383 
               
               
                 T5 + G7 
                 P45 + Q256 
                 V56 + G346 
                 G133 + T355 
                 T193 + S376 
                 G255 + Q385 
               
               
                 T5 + Q11 
                 P45 + A263 
                 V56 + G348 
                 G133 + S376 
                 T193 + D377 
                 G255 + K391 
               
               
                 T5 + N16 
                 P45 + V264 
                 V56 + T355 
                 G133 + D377 
                 T193 + D379 
                 G255 + K393 
               
               
                 T5 + V17 
                 P45 + A265 
                 V56 + S376 
                 G133 + D379 
                 T193 + Y382 
                 G255 + Q395 
               
               
                 T5 + Q32 
                 P45 + Y267 
                 V56 + D377 
                 G133 + Y382 
                 T193 + S383 
                 Q256 + A263 
               
               
                 T5 + A37 
                 P45 + N270 
                 V56 + D379 
                 G133 + S383 
                 T193 + Q385 
                 Q256 + V264 
               
               
                 T5 + T40 
                 P45 + G273 
                 V56 + Y382 
                 G133 + Q385 
                 T193 + K391 
                 Q256 + A265 
               
               
                 T5 + P45 
                 P45 + S280 
                 V56 + S383 
                 G133 + K391 
                 T193 + K393 
                 Q256 + Y267 
               
               
                 T5 + W48 
                 P45 + T285 
                 V56 + Q385 
                 G133 + K393 
                 T193 + Q395 
                 Q256 + N270 
               
               
                 T5 + G50 
                 P45 + M286 
                 V56 + K391 
                 G133 + Q395 
                 N195 + G196 
                 Q256 + G273 
               
               
                 T5 + T51 
                 P45 + F289 
                 V56 + K393 
                 T134 + W140 
                 N195 + A204 
                 Q256 + S280 
               
               
                 T5 + N54 
                 P45 + V291 
                 V56 + Q395 
                 T134 + G142 
                 N195 + V206 
                 Q256 + T285 
               
               
                 T5 + V56 
                 P45 + Q299 
                 A60 + K72 
                 T134 + G149 
                 N195 + P211 
                 Q256 + M286 
               
               
                 T5 + A60 
                 P45 + S304 
                 A60 + R87 
                 T134 + T165 
                 N195 + I214 
                 Q256 + F289 
               
               
                 T5 + K72 
                 P45 + R320 
                 A60 + Q98 
                 T134 + W167 
                 N195 + V215 
                 Q256 + V291 
               
               
                 T5 + R87 
                 P45 + H321 
                 A60 + M105 
                 T134 + R171 
                 N195 + L217 
                 Q256 + Q299 
               
               
                 T5 + Q98 
                 P45 + S323 
                 A60 + G109 
                 T134 + Q172 
                 N195 + L219 
                 Q256 + S304 
               
               
                 T5 + M105 
                 P45 + H324 
                 A60 + F113 
                 T134 + L173 
                 N195 + L228 
                 Q256 + R320 
               
               
                 T5 + G109 
                 P45 + F328 
                 A60 + R116 
                 T134 + A174 
                 N195 + L235 
                 Q256 + H321 
               
               
                 T5 + F113 
                 P45 + T334 
                 A60 + Q118 
                 T134 + G184 
                 N195 + V238 
                 Q256 + S323 
               
               
                 T5 + R116 
                 P45 + D337 
                 A60 + Q125 
                 T134 + T193 
                 N195 + M246 
                 Q256 + H324 
               
               
                 T5 + Q118 
                 P45 + Q345 
                 A60 + G133 
                 T134 + N195 
                 N195 + M248 
                 Q256 + F328 
               
               
                 T5 + Q125 
                 P45 + G346 
                 A60 + T134 
                 T134 + G196 
                 N195 + L250 
                 Q256 + T334 
               
               
                 T5 + G133 
                 P45 + G348 
                 A60 + W140 
                 T134 + A204 
                 N195 + G255 
                 Q256 + D337 
               
               
                 T5 + T134 
                 P45 + T355 
                 A60 + G142 
                 T134 + V206 
                 N195 + Q256 
                 Q256 + Q345 
               
               
                 T5 + W140 
                 P45 + S376 
                 A60 + G149 
                 T134 + P211 
                 N195 + A263 
                 Q256 + G346 
               
               
                 T5 + G142 
                 P45 + D377 
                 A60 + T165 
                 T134 + I214 
                 N195 + V264 
                 Q256 + G348 
               
               
                 T5 + G149 
                 P45 + D379 
                 A60 + W167 
                 T134 + V215 
                 N195 + A265 
                 Q256 + T355 
               
               
                 T5 + T165 
                 P45 + Y382 
                 A60 + R171 
                 T134 + L217 
                 N195 + Y267 
                 Q256 + S376 
               
               
                 T5 + W167 
                 P45 + S383 
                 A60 + Q172 
                 T134 + L219 
                 N195 + N270 
                 Q256 + D377 
               
               
                 T5 + R171 
                 P45 + Q385 
                 A60 + L173 
                 T134 + L228 
                 N195 + G273 
                 Q256 + D379 
               
               
                 T5 + Q172 
                 P45 + K391 
                 A60 + A174 
                 T134 + L235 
                 N195 + S280 
                 Q256 + Y382 
               
               
                 T5 + L173 
                 P45 + K393 
                 A60 + G184 
                 T134 + V238 
                 N195 + T285 
                 Q256 + S383 
               
               
                 T5 + A174 
                 P45 + Q395 
                 A60 + T193 
                 T134 + M246 
                 N195 + M286 
                 Q256 + Q385 
               
               
                 T5 + G184 
                 W48 + G50 
                 A60 + N195 
                 T134 + M248 
                 N195 + F289 
                 Q256 + K391 
               
               
                 T5 + T193 
                 W48 + T51 
                 A60 + G196 
                 T134 + L250 
                 N195 + V291 
                 Q256 + K393 
               
               
                 T5 + N195 
                 W48 + N54 
                 A60 + A204 
                 T134 + G255 
                 N195 + Q299 
                 Q256 + Q395 
               
               
                 T5 + G196 
                 W48 + V56 
                 A60 + V206 
                 T134 + Q256 
                 N195 + S304 
                 A263 + V264 
               
               
                 T5 + A204 
                 W48 + A60 
                 A60 + P211 
                 T134 + A263 
                 N195 + R320 
                 A263 + A265 
               
               
                 T5 + V206 
                 W48 + K72 
                 A60 + I214 
                 T134 + V264 
                 N195 + H321 
                 A263 + Y267 
               
               
                 T5 + P211 
                 W48 + R87 
                 A60 + V215 
                 T134 + A265 
                 N195 + S323 
                 A263 + N270 
               
               
                 T5 + I214 
                 W48 + Q98 
                 A60 + L217 
                 T134 + Y267 
                 N195 + H324 
                 A263 + G273 
               
               
                 T5 + V215 
                 W48 + M105 
                 A60 + L219 
                 T134 + N270 
                 N195 + F328 
                 A263 + S280 
               
               
                 T5 + L217 
                 W48 + G109 
                 A60 + L228 
                 T134 + G273 
                 N195 + T334 
                 A263 + T285 
               
               
                 T5 + L219 
                 W48 + F113 
                 A60 + L235 
                 T134 + S280 
                 N195 + D337 
                 A263 + M286 
               
               
                 T5 + L228 
                 W48 + R116 
                 A60 + V238 
                 T134 + T285 
                 N195 + Q345 
                 A263 + F289 
               
               
                 T5 + L235 
                 W48 + Q118 
                 A60 + M246 
                 T134 + M286 
                 N195 + G346 
                 A263 + V291 
               
               
                 T5 + V238 
                 W48 + Q125 
                 A60 + M248 
                 T134 + F289 
                 N195 + G348 
                 A263 + Q299 
               
               
                 T5 + M246 
                 W48 + G133 
                 A60 + L250 
                 T134 + V291 
                 N195 + T355 
                 A263 + S304 
               
               
                 T5 + M248 
                 W48 + T134 
                 A60 + G255 
                 T134 + Q299 
                 N195 + S376 
                 A263 + R320 
               
               
                 T5 + L250 
                 W48 + W140 
                 A60 + Q256 
                 T134 + S304 
                 N195 + D377 
                 A263 + H321 
               
               
                 T5 + G255 
                 W48 + G142 
                 A60 + A263 
                 T134 + R320 
                 N195 + D379 
                 A263 + S323 
               
               
                 T5 + Q256 
                 W48 + G149 
                 A60 + V264 
                 T134 + H321 
                 N195 + Y382 
                 A263 + H324 
               
               
                 T5 + A263 
                 W48 + T165 
                 A60 + A265 
                 T134 + S323 
                 N195 + S383 
                 A263 + F328 
               
               
                 T5 + V264 
                 W48 + W167 
                 A60 + Y267 
                 T134 + H324 
                 N195 + Q385 
                 A263 + T334 
               
               
                 T5 + A265 
                 W48 + R171 
                 A60 + N270 
                 T134 + F328 
                 N195 + K391 
                 A263 + D337 
               
               
                 T5 + Y267 
                 W48 + Q172 
                 A60 + G273 
                 T134 + T334 
                 N195 + K393 
                 A263 + Q345 
               
               
                 T5 + N270 
                 W48 + L173 
                 A60 + S280 
                 T134 + D337 
                 N195 + Q395 
                 A263 + G346 
               
               
                 T5 + G273 
                 W48 + A174 
                 A60 + T285 
                 T134 + Q345 
                 G196 + A204 
                 A263 + G348 
               
               
                 T5 + S280 
                 W48 + G184 
                 A60 + M286 
                 T134 + G346 
                 G196 + V206 
                 A263 + T355 
               
               
                 T5 + T285 
                 W48 + T193 
                 A60 + F289 
                 T134 + G348 
                 G196 + P211 
                 A263 + S376 
               
               
                 T5 + M286 
                 W48 + N195 
                 A60 + V291 
                 T134 + T355 
                 G196 + I214 
                 A263 + D377 
               
               
                 T5 + F289 
                 W48 + G196 
                 A60 + Q299 
                 T134 + S376 
                 G196 + V215 
                 A263 + D379 
               
               
                 T5 + V291 
                 W48 + A204 
                 A60 + S304 
                 T134 + D377 
                 G196 + L217 
                 A263 + Y382 
               
               
                 T5 + Q299 
                 W48 + V206 
                 A60 + R320 
                 T134 + D379 
                 G196 + L219 
                 A263 + S383 
               
               
                 T5 + S304 
                 W48 + P211 
                 A60 + H321 
                 T134 + Y382 
                 G196 + L228 
                 A263 + Q385 
               
               
                 T5 + R320 
                 W48 + I214 
                 A60 + S323 
                 T134 + S383 
                 G196 + L235 
                 A263 + K391 
               
               
                 T5 + H321 
                 W48 + V215 
                 A60 + H324 
                 T134 + Q385 
                 G196 + V238 
                 A263 + K393 
               
               
                 T5 + S323 
                 W48 + L217 
                 A60 + F328 
                 T134 + K391 
                 G196 + M246 
                 A263 + Q395 
               
               
                 T5 + H324 
                 W48 + L219 
                 A60 + T334 
                 T134 + K393 
                 G196 + M248 
                 V264 + A265 
               
               
                 T5 + F328 
                 W48 + L228 
                 A60 + D337 
                 T134 + Q395 
                 G196 + L250 
                 V264 + Y267 
               
               
                 T5 + T334 
                 W48 + L235 
                 A60 + Q345 
                 W140 + G142 
                 G196 + G255 
                 V264 + N270 
               
               
                 T5 + D337 
                 W48 + V238 
                 A60 + G346 
                 W140 + G149 
                 G196 + Q256 
                 V264 + G273 
               
               
                 T5 + Q345 
                 W48 + M246 
                 A60 + G348 
                 W140 + T165 
                 G196 + A263 
                 V264 + S280 
               
               
                 T5 + G346 
                 W48 + M248 
                 A60 + T355 
                 W140 + W167 
                 G196 + V264 
                 V264 + T285 
               
               
                 T5 + G348 
                 W48 + L250 
                 A60 + S376 
                 W140 + R171 
                 G196 + A265 
                 V264 + M286 
               
               
                 T5 + T355 
                 W48 + G255 
                 A60 + D377 
                 W140 + Q172 
                 G196 + Y267 
                 V264 + F289 
               
               
                 T5 + S376 
                 W48 + Q256 
                 A60 + D379 
                 W140 + L173 
                 G196 + N270 
                 V264 + V291 
               
               
                 T5 + D377 
                 W48 + A263 
                 A60 + Y382 
                 W140 + A174 
                 G196 + G273 
                 V264 + Q299 
               
               
                 T5 + D379 
                 W48 + V264 
                 A60 + S383 
                 W140 + G184 
                 G196 + S280 
                 V264 + S304 
               
               
                 T5 + Y382 
                 W48 + A265 
                 A60 + Q385 
                 W140 + T193 
                 G196 + T285 
                 V264 + R320 
               
               
                 T5 + S383 
                 W48 + Y267 
                 A60 + K391 
                 W140 + N195 
                 G196 + M286 
                 V264 + H321 
               
               
                 T5 + Q385 
                 W48 + N270 
                 A60 + K393 
                 W140 + G196 
                 G196 + F289 
                 V264 + S323 
               
               
                 T5 + K391 
                 W48 + G273 
                 A60 + Q395 
                 W140 + A204 
                 G196 + V291 
                 V264 + H324 
               
               
                 T5 + K393 
                 W48 + S280 
                 K72 + R87 
                 W140 + V206 
                 G196 + Q299 
                 V264 + F328 
               
               
                 T5 + Q395 
                 W48 + T285 
                 K72 + Q98 
                 W140 + P211 
                 G196 + S304 
                 V264 + T334 
               
               
                 G7 + Q11 
                 W48 + M286 
                 K72 + M105 
                 W140 + I214 
                 G196 + R320 
                 V264 + D337 
               
               
                 G7 + N16 
                 W48 + F289 
                 K72 + G109 
                 W140 + V215 
                 G196 + H321 
                 V264 + Q345 
               
               
                 G7 + V17 
                 W48 + V291 
                 K72 + F113 
                 W140 + L217 
                 G196 + S323 
                 V264 + G346 
               
               
                 G7 + Q32 
                 W48 + Q299 
                 K72 + R116 
                 W140 + L219 
                 G196 + H324 
                 V264 + G348 
               
               
                 G7 + A37 
                 W48 + S304 
                 K72 + Q118 
                 W140 + L228 
                 G196 + F328 
                 V264 + T355 
               
               
                 G7 + T40 
                 W48 + R320 
                 K72 + Q125 
                 W140 + L235 
                 G196 + T334 
                 V264 + S376 
               
               
                 G7 + P45 
                 W48 + H321 
                 K72 + G133 
                 W140 + V238 
                 G196 + D337 
                 V264 + D377 
               
               
                 G7 + W48 
                 W48 + S323 
                 K72 + T134 
                 W140 + M246 
                 G196 + Q345 
                 V264 + D379 
               
               
                 G7 + G50 
                 W48 + H324 
                 K72 + W140 
                 W140 + M248 
                 G196 + G346 
                 V264 + Y382 
               
               
                 G7 + T51 
                 W48 + F328 
                 K72 + G142 
                 W140 + L250 
                 G196 + G348 
                 V264 + S383 
               
               
                 G7 + N54 
                 W48 + T334 
                 K72 + G149 
                 W140 + G255 
                 G196 + T355 
                 V264 + Q385 
               
               
                 G7 + V56 
                 W48 + D337 
                 K72 + T165 
                 W140 + Q256 
                 G196 + S376 
                 V264 + K391 
               
               
                 G7 + A60 
                 W48 + Q345 
                 K72 + W167 
                 W140 + A263 
                 G196 + D377 
                 V264 + K393 
               
               
                 G7 + K72 
                 W48 + G346 
                 K72 + R171 
                 W140 + V264 
                 G196 + D379 
                 V264 + Q395 
               
               
                 G7 + R87 
                 W48 + G348 
                 K72 + Q172 
                 W140 + A265 
                 G196 + Y382 
                 A265 + Y267 
               
               
                 G7 + Q98 
                 W48 + T355 
                 K72 + L173 
                 W140 + Y267 
                 G196 + S383 
                 A265 + N270 
               
               
                 G7 + M105 
                 W48 + S376 
                 K72 + A174 
                 W140 + N270 
                 G196 + Q385 
                 A265 + G273 
               
               
                 G7 + G109 
                 W48 + D377 
                 K72 + G184 
                 W140 + G273 
                 G196 + K391 
                 A265 + S280 
               
               
                 G7 + F113 
                 W48 + D379 
                 K72 + T193 
                 W140 + S280 
                 G196 + K393 
                 A265 + T285 
               
               
                 G7 + R116 
                 W48 + Y382 
                 K72 + N195 
                 W140 + T285 
                 G196 + Q395 
                 A265 + M286 
               
               
                 G7 + Q118 
                 W48 + S383 
                 K72 + G196 
                 W140 + M286 
                 A204 + V206 
                 A265 + F289 
               
               
                 G7 + Q125 
                 W48 + Q385 
                 K72 + A204 
                 W140 + F289 
                 A204 + P211 
                 A265 + V291 
               
               
                 G7 + G133 
                 W48 + K391 
                 K72 + V206 
                 W140 + V291 
                 A204 + I214 
                 A265 + Q299 
               
               
                 G7 + T134 
                 W48 + K393 
                 K72 + P211 
                 W140 + Q299 
                 A204 + V215 
                 A265 + S304 
               
               
                 G7 + W140 
                 W48 + Q395 
                 K72 + I214 
                 W140 + S304 
                 A204 + L217 
                 A265 + R320 
               
               
                 G7 + G142 
                 G50 + T51 
                 K72 + V215 
                 W140 + R320 
                 A204 + L219 
                 A265 + H321 
               
               
                 G7 + G149 
                 G50 + N54 
                 K72 + L217 
                 W140 + H321 
                 A204 + L228 
                 A265 + S323 
               
               
                 G7 + T165 
                 G50 + V56 
                 K72 + L219 
                 W140 + S323 
                 A204 + L235 
                 A265 + H324 
               
               
                 G7 + W167 
                 G50 + A60 
                 K72 + L228 
                 W140 + H324 
                 A204 + V238 
                 A265 + F328 
               
               
                 G7 + R171 
                 G50 + K72 
                 K72 + L235 
                 W140 + F328 
                 A204 + M246 
                 A265 + T334 
               
               
                 G7 + Q172 
                 G50 + R87 
                 K72 + V238 
                 W140 + T334 
                 A204 + M248 
                 A265 + D337 
               
               
                 G7 + L173 
                 G50 + Q98 
                 K72 + M246 
                 W140 + D337 
                 A204 + L250 
                 A265 + Q345 
               
               
                 G7 + A174 
                 G50 + M105 
                 K72 + M248 
                 W140 + Q345 
                 A204 + G255 
                 A265 + G346 
               
               
                 G7 + G184 
                 G50 + G109 
                 K72 + L250 
                 W140 + G346 
                 A204 + Q256 
                 A265 + G348 
               
               
                 G7 + T193 
                 G50 + F113 
                 K72 + G255 
                 W140 + G348 
                 A204 + A263 
                 A265 + T355 
               
               
                 G7 + N195 
                 G50 + R116 
                 K72 + Q256 
                 W140 + T355 
                 A204 + V264 
                 A265 + S376 
               
               
                 G7 + G196 
                 G50 + Q118 
                 K72 + A263 
                 W140 + S376 
                 A204 + A265 
                 A265 + D377 
               
               
                 G7 + A204 
                 G50 + Q125 
                 K72 + V264 
                 W140 + D377 
                 A204 + Y267 
                 A265 + D379 
               
               
                 G7 + V206 
                 G50 + G133 
                 K72 + A265 
                 W140 + D379 
                 A204 + N270 
                 A265 + Y382 
               
               
                 G7 + P211 
                 G50 + T134 
                 K72 + Y267 
                 W140 + Y382 
                 A204 + G273 
                 A265 + S383 
               
               
                 G7 + I214 
                 G50 + W140 
                 K72 + N270 
                 W140 + S383 
                 A204 + S280 
                 A265 + Q385 
               
               
                 G7 + V215 
                 G50 + G142 
                 K72 + G273 
                 W140 + Q385 
                 A204 + T285 
                 A265 + K391 
               
               
                 G7 + L217 
                 G50 + G149 
                 K72 + S280 
                 W140 + K391 
                 A204 + M286 
                 A265 + K393 
               
               
                 G7 + L219 
                 G50 + T165 
                 K72 + T285 
                 W140 + K393 
                 A204 + F289 
                 A265 + Q395 
               
               
                 G7 + L228 
                 G50 + W167 
                 K72 + M286 
                 W140 + Q395 
                 A204 + V291 
                 Y267 + N270 
               
               
                 G7 + L235 
                 G50 + R171 
                 K72 + F289 
                 G142 + G149 
                 A204 + Q299 
                 Y267 + G273 
               
               
                 G7 + V238 
                 G50 + Q172 
                 K72 + V291 
                 G142 + T165 
                 A204 + S304 
                 Y267 + S280 
               
               
                 G7 + M246 
                 G50 + L173 
                 K72 + Q299 
                 G142 + W167 
                 A204 + R320 
                 Y267 + T285 
               
               
                 G7 + M248 
                 G50 + A174 
                 K72 + S304 
                 G142 + R171 
                 A204 + H321 
                 Y267 + M286 
               
               
                 G7 + L250 
                 G50 + G184 
                 K72 + R320 
                 G142 + Q172 
                 A204 + S323 
                 Y267 + F289 
               
               
                 G7 + G255 
                 G50 + T193 
                 K72 + H321 
                 G142 + L173 
                 A204 + H324 
                 Y267 + V291 
               
               
                 G7 + Q256 
                 G50 + N195 
                 K72 + S323 
                 G142 + A174 
                 A204 + F328 
                 Y267 + Q299 
               
               
                 G7 + A263 
                 G50 + G196 
                 K72 + H324 
                 G142 + G184 
                 A204 + T334 
                 Y267 + S304 
               
               
                 G7 + V264 
                 G50 + A204 
                 K72 + F328 
                 G142 + T193 
                 A204 + D337 
                 Y267 + R320 
               
               
                 G7 + A265 
                 G50 + V206 
                 K72 + T334 
                 G142 + N195 
                 A204 + Q345 
                 Y267 + H321 
               
               
                 G7 + Y267 
                 G50 + P211 
                 K72 + D337 
                 G142 + G196 
                 A204 + G346 
                 Y267 + S323 
               
               
                 G7 + N270 
                 G50 + I214 
                 K72 + Q345 
                 G142 + A204 
                 A204 + G348 
                 Y267 + H324 
               
               
                 G7 + G273 
                 G50 + V215 
                 K72 + G346 
                 G142 + V206 
                 A204 + T355 
                 Y267 + F328 
               
               
                 G7 + S280 
                 G50 + L217 
                 K72 + G348 
                 G142 + P211 
                 A204 + S376 
                 Y267 + T334 
               
               
                 G7 + T285 
                 G50 + L219 
                 K72 + T355 
                 G142 + I214 
                 A204 + D377 
                 Y267 + D337 
               
               
                 G7 + M286 
                 G50 + L228 
                 K72 + S376 
                 G142 + V215 
                 A204 + D379 
                 Y267 + Q345 
               
               
                 G7 + F289 
                 G50 + L235 
                 K72 + D377 
                 G142 + L217 
                 A204 + Y382 
                 Y267 + G346 
               
               
                 G7 + V291 
                 G50 + V238 
                 K72 + D379 
                 G142 + L219 
                 A204 + S383 
                 Y267 + G348 
               
               
                 G7 + Q299 
                 G50 + M246 
                 K72 + Y382 
                 G142 + L228 
                 A204 + Q385 
                 Y267 + T355 
               
               
                 G7 + S304 
                 G50 + M248 
                 K72 + S383 
                 G142 + L235 
                 A204 + K391 
                 Y267 + S376 
               
               
                 G7 + R320 
                 G50 + L250 
                 K72 + Q385 
                 G142 + V238 
                 A204 + K393 
                 Y267 + D377 
               
               
                 G7 + H321 
                 G50 + G255 
                 K72 + K391 
                 G142 + M246 
                 A204 + Q395 
                 Y267 + D379 
               
               
                 G7 + S323 
                 G50 + Q256 
                 K72 + K393 
                 G142 + M248 
                 V206 + P211 
                 Y267 + Y382 
               
               
                 G7 + H324 
                 G50 + A263 
                 K72 + Q395 
                 G142 + L250 
                 V206 + I214 
                 Y267 + S383 
               
               
                 G7 + F328 
                 G50 + V264 
                 R87 + Q98 
                 G142 + G255 
                 V206 + V215 
                 Y267 + Q385 
               
               
                 G7 + T334 
                 G50 + A265 
                 R87 + M105 
                 G142 + Q256 
                 V206 + L217 
                 Y267 + K391 
               
               
                 G7 + D337 
                 G50 + Y267 
                 R87 + G109 
                 G142 + A263 
                 V206 + L219 
                 Y267 + K393 
               
               
                 G7 + Q345 
                 G50 + N270 
                 R87 + F113 
                 G142 + V264 
                 V206 + L228 
                 Y267 + Q395 
               
               
                 G7 + G346 
                 G50 + G273 
                 R87 + R116 
                 G142 + A265 
                 V206 + L235 
                 N270 + G273 
               
               
                 G7 + G348 
                 G50 + S280 
                 R87 + Q118 
                 G142 + Y267 
                 V206 + V238 
                 N270 + S280 
               
               
                 G7 + T355 
                 G50 + T285 
                 R87 + Q125 
                 G142 + N270 
                 V206 + M246 
                 N270 + T285 
               
               
                 G7 + S376 
                 G50 + M286 
                 R87 + G133 
                 G142 + G273 
                 V206 + M248 
                 N270 + M286 
               
               
                 G7 + D377 
                 G50 + F289 
                 R87 + T134 
                 G142 + S280 
                 V206 + L250 
                 N270 + F289 
               
               
                 G7 + D379 
                 G50 + V291 
                 R87 + W140 
                 G142 + T285 
                 V206 + G255 
                 N270 + V291 
               
               
                 G7 + Y382 
                 G50 + Q299 
                 R87 + G142 
                 G142 + M286 
                 V206 + Q256 
                 N270 + Q299 
               
               
                 G7 + S383 
                 G50 + S304 
                 R87 + G149 
                 G142 + F289 
                 V206 + A263 
                 N270 + S304 
               
               
                 G7 + Q385 
                 G50 + R320 
                 R87 + T165 
                 G142 + V291 
                 V206 + V264 
                 N270 + R320 
               
               
                 G7 + K391 
                 G50 + H321 
                 R87 + W167 
                 G142 + Q299 
                 V206 + A265 
                 N270 + H321 
               
               
                 G7 + K393 
                 G50 + S323 
                 R87 + R171 
                 G142 + S304 
                 V206 + Y267 
                 N270 + S323 
               
               
                 G7 + Q395 
                 G50 + H324 
                 R87 + Q172 
                 G142 + R320 
                 V206 + N270 
                 N270 + H324 
               
               
                 Q11 + N16 
                 G50 + F328 
                 R87 + L173 
                 G142 + H321 
                 V206 + G273 
                 N270 + F328 
               
               
                 Q11 + V17 
                 G50 + T334 
                 R87 + A174 
                 G142 + S323 
                 V206 + S280 
                 N270 + T334 
               
               
                 Q11 + Q32 
                 G50 + D337 
                 R87 + G184 
                 G142 + H324 
                 V206 + T285 
                 N270 + D337 
               
               
                 Q11 + A37 
                 G50 + Q345 
                 R87 + T193 
                 G142 + F328 
                 V206 + M286 
                 N270 + Q345 
               
               
                 Q11 + T40 
                 G50 + G346 
                 R87 + N195 
                 G142 + T334 
                 V206 + F289 
                 N270 + G346 
               
               
                 Q11 + P45 
                 G50 + G348 
                 R87 + G196 
                 G142 + D337 
                 V206 + V291 
                 N270 + G348 
               
               
                 Q11 + W48 
                 G50 + T355 
                 R87 + A204 
                 G142 + Q345 
                 V206 + Q299 
                 N270 + T355 
               
               
                 Q11 + G50 
                 G50 + S376 
                 R87 + V206 
                 G142 + G346 
                 V206 + S304 
                 N270 + S376 
               
               
                 Q11 + T51 
                 G50 + D377 
                 R87 + P211 
                 G142 + G348 
                 V206 + R320 
                 N270 + D377 
               
               
                 Q11 + N54 
                 G50 + D379 
                 R87 + I214 
                 G142 + T355 
                 V206 + H321 
                 N270 + D379 
               
               
                 Q11 + V56 
                 G50 + Y382 
                 R87 + V215 
                 G142 + S376 
                 V206 + S323 
                 N270 + Y382 
               
               
                 Q11 + A60 
                 G50 + S383 
                 R87 + L217 
                 G142 + D377 
                 V206 + H324 
                 N270 + S383 
               
               
                 Q11 + K72 
                 G50 + Q385 
                 R87 + L219 
                 G142 + D379 
                 V206 + F328 
                 N270 + Q385 
               
               
                 Q11 + R87 
                 G50 + K391 
                 R87 + L228 
                 G142 + Y382 
                 V206 + T334 
                 N270 + K391 
               
               
                 Q11 + Q98 
                 G50 + K393 
                 R87 + L235 
                 G142 + S383 
                 V206 + D337 
                 N270 + K393 
               
               
                 Q11 + M105 
                 G50 + Q395 
                 R87 + V238 
                 G142 + Q385 
                 V206 + Q345 
                 N270 + Q395 
               
               
                 Q11 + G109 
                 T51 + N54 
                 R87 + M246 
                 G142 + K391 
                 V206 + G346 
                 G273 + S280 
               
               
                 Q11 + F113 
                 T51 + V56 
                 R87 + M248 
                 G142 + K393 
                 V206 + G348 
                 G273 + T285 
               
               
                 Q11 + R116 
                 T51 + A60 
                 R87 + L250 
                 G142 + Q395 
                 V206 + T355 
                 G273 + M286 
               
               
                 Q11 + Q118 
                 T51 + K72 
                 R87 + G255 
                 G149 + T165 
                 V206 + S376 
                 G273 + F289 
               
               
                 Q11 + Q125 
                 T51 + R87 
                 R87 + Q256 
                 G149 + W167 
                 V206 + D377 
                 G273 + V291 
               
               
                 Q11 + G133 
                 T51 + Q98 
                 R87 + A263 
                 G149 + R171 
                 V206 + D379 
                 G273 + Q299 
               
               
                 Q11 + T134 
                 T51 + M105 
                 R87 + V264 
                 G149 + Q172 
                 V206 + Y382 
                 G273 + S304 
               
               
                 Q11 + W140 
                 T51 + G109 
                 R87 + A265 
                 G149 + L173 
                 V206 + S383 
                 G273 + R320 
               
               
                 Q11 + G142 
                 T51 + F113 
                 R87 + Y267 
                 G149 + A174 
                 V206 + Q385 
                 G273 + H321 
               
               
                 Q11 + G149 
                 T51 + R116 
                 R87 + N270 
                 G149 + G184 
                 V206 + K391 
                 G273 + S323 
               
               
                 Q11 + T165 
                 T51 + Q118 
                 R87 + G273 
                 G149 + T193 
                 V206 + K393 
                 G273 + H324 
               
               
                 Q11 + W167 
                 T51 + Q125 
                 R87 + S280 
                 G149 + N195 
                 V206 + Q395 
                 G273 + F328 
               
               
                 Q11 + R171 
                 T51 + G133 
                 R87 + T285 
                 G149 + G196 
                 P211 + I214 
                 G273 + T334 
               
               
                 Q11 + Q172 
                 T51 + T134 
                 R87 + M286 
                 G149 + A204 
                 P211 + V215 
                 G273 + D337 
               
               
                 Q11 + L173 
                 T51 + W140 
                 R87 + F289 
                 G149 + V206 
                 P211 + L217 
                 G273 + Q345 
               
               
                 Q11 + A174 
                 T51 + G142 
                 R87 + V291 
                 G149 + P211 
                 P211 + L219 
                 G273 + G346 
               
               
                 Q11 + G184 
                 T51 + G149 
                 R87 + Q299 
                 G149 + I214 
                 P211 + L228 
                 G273 + G348 
               
               
                 Q11 + T193 
                 T51 + T165 
                 R87 + S304 
                 G149 + V215 
                 P211 + L235 
                 G273 + T355 
               
               
                 Q11 + N195 
                 T51 + W167 
                 R87 + R320 
                 G149 + L217 
                 P211 + V238 
                 G273 + S376 
               
               
                 Q11 + G196 
                 T51 + R171 
                 R87 + H321 
                 G149 + L219 
                 P211 + M246 
                 G273 + D377 
               
               
                 Q11 + A204 
                 T51 + Q172 
                 R87 + S323 
                 G149 + L228 
                 P211 + M248 
                 G273 + D379 
               
               
                 Q11 + V206 
                 T51 + L173 
                 R87 + H324 
                 G149 + L235 
                 P211 + L250 
                 G273 + Y382 
               
               
                 Q11 + P211 
                 T51 + A174 
                 R87 + F328 
                 G149 + V238 
                 P211 + G255 
                 G273 + S383 
               
               
                 Q11 + I214 
                 T51 + G184 
                 R87 + T334 
                 G149 + M246 
                 P211 + Q256 
                 G273 + Q385 
               
               
                 Q11 + V215 
                 T51 + T193 
                 R87 + D337 
                 G149 + M248 
                 P211 + A263 
                 G273 + K391 
               
               
                 Q11 + L217 
                 T51 + N195 
                 R87 + Q345 
                 G149 + L250 
                 P211 + V264 
                 G273 + K393 
               
               
                 Q11 + L219 
                 T51 + G196 
                 R87 + G346 
                 G149 + G255 
                 P211 + A265 
                 G273 + Q395 
               
               
                 Q11 + L228 
                 T51 + A204 
                 R87 + G348 
                 G149 + Q256 
                 P211 + Y267 
                 S280 + T285 
               
               
                 Q11 + L235 
                 T51 + V206 
                 R87 + T355 
                 G149 + A263 
                 P211 + N270 
                 S280 + M286 
               
               
                 Q11 + V238 
                 T51 + P211 
                 R87 + S376 
                 G149 + V264 
                 P211 + G273 
                 S280 + F289 
               
               
                 Q11 + M246 
                 T51 + I214 
                 R87 + D377 
                 G149 + A265 
                 P211 + S280 
                 S280 + V291 
               
               
                 Q11 + M248 
                 T51 + V215 
                 R87 + D379 
                 G149 + Y267 
                 P211 + T285 
                 S280 + Q299 
               
               
                 Q11 + L250 
                 T51 + L217 
                 R87 + Y382 
                 G149 + N270 
                 P211 + M286 
                 S280 + S304 
               
               
                 Q11 + G255 
                 T51 + L219 
                 R87 + S383 
                 G149 + G273 
                 P211 + F289 
                 S280 + R320 
               
               
                 Q11 + Q256 
                 T51 + L228 
                 R87 + Q385 
                 G149 + S280 
                 P211 + V291 
                 S280 + H321 
               
               
                 Q11 + A263 
                 T51 + L235 
                 R87 + K391 
                 G149 + T285 
                 P211 + Q299 
                 S280 + S323 
               
               
                 Q11 + V264 
                 T51 + V238 
                 R87 + K393 
                 G149 + M286 
                 P211 + S304 
                 S280 + H324 
               
               
                 Q11 + A265 
                 T51 + M246 
                 R87 + Q395 
                 G149 + F289 
                 P211 + R320 
                 S280 + F328 
               
               
                 Q11 + Y267 
                 T51 + M248 
                 Q98 + M105 
                 G149 + V291 
                 P211 + H321 
                 S280 + T334 
               
               
                 Q11 + N270 
                 T51 + L250 
                 Q98 + G109 
                 G149 + Q299 
                 P211 + S323 
                 S280 + D337 
               
               
                 Q11 + G273 
                 T51 + G255 
                 Q98 + F113 
                 G149 + S304 
                 P211 + H324 
                 S280 + Q345 
               
               
                 Q11 + S280 
                 T51 + Q256 
                 Q98 + R116 
                 G149 + R320 
                 P211 + F328 
                 S280 + G346 
               
               
                 Q11 + T285 
                 T51 + A263 
                 Q98 + Q118 
                 G149 + H321 
                 P211 + T334 
                 S280 + G348 
               
               
                 Q11 + M286 
                 T51 + V264 
                 Q98 + Q125 
                 G149 + S323 
                 P211 + D337 
                 S280 + T355 
               
               
                 Q11 + F289 
                 T51 + A265 
                 Q98 + G133 
                 G149 + H324 
                 P211 + Q345 
                 S280 + S376 
               
               
                 Q11 + V291 
                 T51 + Y267 
                 Q98 + T134 
                 G149 + F328 
                 P211 + G346 
                 S280 + D377 
               
               
                 Q11 + Q299 
                 T51 + N270 
                 Q98 + W140 
                 G149 + T334 
                 P211 + G348 
                 S280 + D379 
               
               
                 Q11 + S304 
                 T51 + G273 
                 Q98 + G142 
                 G149 + D337 
                 P211 + T355 
                 S280 + Y382 
               
               
                 Q11 + R320 
                 T51 + S280 
                 Q98 + G149 
                 G149 + Q345 
                 P211 + S376 
                 S280 + S383 
               
               
                 Q11 + H321 
                 T51 + T285 
                 Q98 + T165 
                 G149 + G346 
                 P211 + D377 
                 S280 + Q385 
               
               
                 Q11 + S323 
                 T51 + M286 
                 Q98 + W167 
                 G149 + G348 
                 P211 + D379 
                 S280 + K391 
               
               
                 Q11 + H324 
                 T51 + F289 
                 Q98 + R171 
                 G149 + T355 
                 P211 + Y382 
                 S280 + K393 
               
               
                 Q11 + F328 
                 T51 + V291 
                 Q98 + Q172 
                 G149 + S376 
                 P211 + S383 
                 S280 + Q395 
               
               
                 Q11 + T334 
                 T51 + Q299 
                 Q98 + L173 
                 G149 + D377 
                 P211 + Q385 
                 T285 + M286 
               
               
                 Q11 + D337 
                 T51 + S304 
                 Q98 + A174 
                 G149 + D379 
                 P211 + K391 
                 T285 + F289 
               
               
                 Q11 + Q345 
                 T51 + R320 
                 Q98 + G184 
                 G149 + Y382 
                 P211 + K393 
                 T285 + V291 
               
               
                 Q11 + G346 
                 T51 + H321 
                 Q98 + T193 
                 G149 + S383 
                 P211 + Q395 
                 T285 + Q299 
               
               
                 Q11 + G348 
                 T51 + S323 
                 Q98 + N195 
                 G149 + Q385 
                 I214 + V215 
                 T285 + S304 
               
               
                 Q11 + T355 
                 T51 + H324 
                 Q98 + G196 
                 G149 + K391 
                 I214 + L217 
                 T285 + R320 
               
               
                 Q11 + S376 
                 T51 + F328 
                 Q98 + A204 
                 G149 + K393 
                 I214 + L219 
                 T285 + H321 
               
               
                 Q11 + D377 
                 T51 + T334 
                 Q98 + V206 
                 G149 + Q395 
                 I214 + L228 
                 T285 + S323 
               
               
                 Q11 + D379 
                 T51 + D337 
                 Q98 + P211 
                 T165 + W167 
                 I214 + L235 
                 T285 + H324 
               
               
                 Q11 + Y382 
                 T51 + Q345 
                 Q98 + I214 
                 T165 + R171 
                 I214 + V238 
                 T285 + F328 
               
               
                 Q11 + S383 
                 T51 + G346 
                 Q98 + V215 
                 T165 + Q172 
                 I214 + M246 
                 T285 + T334 
               
               
                 Q11 + Q385 
                 T51 + G348 
                 Q98 + L217 
                 T165 + L173 
                 I214 + M248 
                 T285 + D337 
               
               
                 Q11 + K391 
                 T51 + T355 
                 Q98 + L219 
                 T165 + A174 
                 I214 + L250 
                 T285 + Q345 
               
               
                 Q11 + K393 
                 T51 + S376 
                 Q98 + L228 
                 T165 + G184 
                 I214 + G255 
                 T285 + G346 
               
               
                 Q11 + Q395 
                 T51 + D377 
                 Q98 + L235 
                 T165 + T193 
                 I214 + Q256 
                 T285 + G348 
               
               
                 N16 + V17 
                 T51 + D379 
                 Q98 + V238 
                 T165 + N195 
                 I214 + A263 
                 T285 + T355 
               
               
                 N16 + Q32 
                 T51 + Y382 
                 Q98 + M246 
                 T165 + G196 
                 I214 + V264 
                 T285 + S376 
               
               
                 N16 + A37 
                 T51 + S383 
                 Q98 + M248 
                 T165 + A204 
                 I214 + A265 
                 T285 + D377 
               
               
                 N16 + T40 
                 T51 + Q385 
                 Q98 + L250 
                 T165 + V206 
                 I214 + Y267 
                 T285 + D379 
               
               
                 N16 + P45 
                 T51 + K391 
                 Q98 + G255 
                 T165 + P211 
                 I214 + N270 
                 T285 + Y382 
               
               
                 N16 + W48 
                 T51 + K393 
                 Q98 + Q256 
                 T165 + I214 
                 I214 + G273 
                 T285 + S383 
               
               
                 N16 + G50 
                 T51 + Q395 
                 Q98 + A263 
                 T165 + V215 
                 I214 + S280 
                 T285 + Q385 
               
               
                 N16 + T51 
                 N54 + V56 
                 Q98 + V264 
                 T165 + L217 
                 I214 + T285 
                 T285 + K391 
               
               
                 N16 + N54 
                 N54 + A60 
                 Q98 + A265 
                 T165 + L219 
                 I214 + M286 
                 T285 + K393 
               
               
                 N16 + V56 
                 N54 + K72 
                 Q98 + Y267 
                 T165 + L228 
                 I214 + F289 
                 T285 + Q395 
               
               
                 N16 + A60 
                 N54 + R87 
                 Q98 + N270 
                 T165 + L235 
                 I214 + V291 
                 M286 + F289 
               
               
                 N16 + K72 
                 N54 + Q98 
                 Q98 + G273 
                 T165 + V238 
                 I214 + Q299 
                 M286 + V291 
               
               
                 N16 + R87 
                 N54 + M105 
                 Q98 + S280 
                 T165 + M246 
                 I214 + S304 
                 M286 + Q299 
               
               
                 N16 + Q98 
                 N54 + G109 
                 Q98 + T285 
                 T165 + M248 
                 I214 + R320 
                 M286 + S304 
               
               
                 N16 + M105 
                 N54 + F113 
                 Q98 + M286 
                 T165 + L250 
                 I214 + H321 
                 M286 + R320 
               
               
                 N16 + G109 
                 N54 + R116 
                 Q98 + F289 
                 T165 + G255 
                 I214 + S323 
                 M286 + H321 
               
               
                 N16 + F113 
                 N54 + Q118 
                 Q98 + V291 
                 T165 + Q256 
                 I214 + H324 
                 M286 + S323 
               
               
                 N16 + R116 
                 N54 + Q125 
                 Q98 + Q299 
                 T165 + A263 
                 I214 + F328 
                 M286 + H324 
               
               
                 N16 + Q118 
                 N54 + G133 
                 Q98 + S304 
                 T165 + V264 
                 I214 + T334 
                 M286 + F328 
               
               
                 N16 + Q125 
                 N54 + T134 
                 Q98 + R320 
                 T165 + A265 
                 I214 + D337 
                 M286 + T334 
               
               
                 N16 + G133 
                 N54 + W140 
                 Q98 + H321 
                 T165 + Y267 
                 I214 + Q345 
                 M286 + D337 
               
               
                 N16 + T134 
                 N54 + G142 
                 Q98 + S323 
                 T165 + N270 
                 I214 + G346 
                 M286 + Q345 
               
               
                 N16 + W140 
                 N54 + G149 
                 Q98 + H324 
                 T165 + G273 
                 I214 + G348 
                 M286 + G346 
               
               
                 N16 + G142 
                 N54 + T165 
                 Q98 + F328 
                 T165 + S280 
                 I214 + T355 
                 M286 + G348 
               
               
                 N16 + G149 
                 N54 + W167 
                 Q98 + T334 
                 T165 + T285 
                 I214 + S376 
                 M286 + T355 
               
               
                 N16 + T165 
                 N54 + R171 
                 Q98 + D337 
                 T165 + M286 
                 I214 + D377 
                 M286 + S376 
               
               
                 N16 + W167 
                 N54 + Q172 
                 Q98 + Q345 
                 T165 + F289 
                 I214 + D379 
                 M286 + D377 
               
               
                 N16 + R171 
                 N54 + L173 
                 Q98 + G346 
                 T165 + V291 
                 I214 + Y382 
                 M286 + D379 
               
               
                 N16 + Q172 
                 N54 + A174 
                 Q98 + G348 
                 T165 + Q299 
                 I214 + S383 
                 M286 + Y382 
               
               
                 N16 + L173 
                 N54 + G184 
                 Q98 + T355 
                 T165 + S304 
                 I214 + Q385 
                 M286 + S383 
               
               
                 N16 + A174 
                 N54 + T193 
                 Q98 + S376 
                 T165 + R320 
                 I214 + K391 
                 M286 + Q385 
               
               
                 N16 + G184 
                 N54 + N195 
                 Q98 + D377 
                 T165 + H321 
                 I214 + K393 
                 M286 + K391 
               
               
                 N16 + T193 
                 N54 + G196 
                 Q98 + D379 
                 T165 + S323 
                 I214 + Q395 
                 M286 + K393 
               
               
                 N16 + N195 
                 N54 + A204 
                 Q98 + Y382 
                 T165 + H324 
                 V215 + L217 
                 M286 + Q395 
               
               
                 N16 + G196 
                 N54 + V206 
                 Q98 + S383 
                 T165 + F328 
                 V215 + L219 
                 F289 + V291 
               
               
                 N16 + A204 
                 N54 + P211 
                 Q98 + Q385 
                 T165 + T334 
                 V215 + L228 
                 F289 + Q299 
               
               
                 N16 + V206 
                 N54 + I214 
                 Q98 + K391 
                 T165 + D337 
                 V215 + L235 
                 F289 + S304 
               
               
                 N16 + P211 
                 N54 + V215 
                 Q98 + K393 
                 T165 + Q345 
                 V215 + V238 
                 F289 + R320 
               
               
                 N16 + I214 
                 N54 + L217 
                 Q98 + Q395 
                 T165 + G346 
                 V215 + M246 
                 F289 + H321 
               
               
                 N16 + V215 
                 N54 + L219 
                 M105 + G109 
                 T165 + G348 
                 V215 + M248 
                 F289 + S323 
               
               
                 N16 + L217 
                 N54 + L228 
                 M105 + F113 
                 T165 + T355 
                 V215 + L250 
                 F289 + H324 
               
               
                 N16 + L219 
                 N54 + L235 
                 M105 + R116 
                 T165 + S376 
                 V215 + G255 
                 F289 + F328 
               
               
                 N16 + L228 
                 N54 + V238 
                 M105 + Q118 
                 T165 + D377 
                 V215 + Q256 
                 F289 + T334 
               
               
                 N16 + L235 
                 N54 + M246 
                 M105 + Q125 
                 T165 + D379 
                 V215 + A263 
                 F289 + D337 
               
               
                 N16 + V238 
                 N54 + M248 
                 M105 + G133 
                 T165 + Y382 
                 V215 + V264 
                 F289 + Q345 
               
               
                 N16 + M246 
                 N54 + L250 
                 M105 + T134 
                 T165 + S383 
                 V215 + A265 
                 F289 + G346 
               
               
                 N16 + M248 
                 N54 + G255 
                 M105 + W140 
                 T165 + Q385 
                 V215 + Y267 
                 F289 + G348 
               
               
                 N16 + L250 
                 N54 + Q256 
                 M105 + G142 
                 T165 + K391 
                 V215 + N270 
                 F289 + T355 
               
               
                 N16 + G255 
                 N54 + A263 
                 M105 + G149 
                 T165 + K393 
                 V215 + G273 
                 F289 + S376 
               
               
                 N16 + Q256 
                 N54 + V264 
                 M105 + T165 
                 T165 + Q395 
                 V215 + S280 
                 F289 + D377 
               
               
                 N16 + A263 
                 N54 + A265 
                 M105 + W167 
                 W167 + R171 
                 V215 + T285 
                 F289 + D379 
               
               
                 N16 + V264 
                 N54 + Y267 
                 M105 + R171 
                 W167 + Q172 
                 V215 + M286 
                 F289 + Y382 
               
               
                 N16 + A265 
                 N54 + N270 
                 M105 + Q172 
                 W167 + L173 
                 V215 + F289 
                 F289 + S383 
               
               
                 N16 + Y267 
                 N54 + G273 
                 M105 + L173 
                 W167 + A174 
                 V215 + V291 
                 F289 + Q385 
               
               
                 N16 + N270 
                 N54 + S280 
                 M105 + A174 
                 W167 + G184 
                 V215 + Q299 
                 F289 + K391 
               
               
                 N16 + G273 
                 Q118 + V215 
                 M105 + G184 
                 W167 + T193 
                 V215 + S304 
                 F289 + K393 
               
               
                 N16 + S280 
                 Q118 + L217 
                 M105 + T193 
                 W167 + N195 
                 V215 + R320 
                 F289 + Q395 
               
               
                 N16 + T285 
                 Q118 + L219 
                 M105 + N195 
                 W167 + G196 
                 V215 + H321 
                 V291 + Q299 
               
               
                 N16 + M286 
                 Q118 + L228 
                 M105 + G196 
                 W167 + A204 
                 V215 + S323 
                 V291 + S304 
               
               
                 N16 + F289 
                 Q118 + L235 
                 M105 + A204 
                 W167 + V206 
                 V215 + H324 
                 V291 + R320 
               
               
                 N16 + V291 
                 Q118 + V238 
                 M105 + V206 
                 W167 + P211 
                 V215 + F328 
                 V291 + H321 
               
               
                 N16 + Q299 
                 Q118 + M246 
                 M105 + P211 
                 W167 + I214 
                 V215 + T334 
                 V291 + S323 
               
               
                 N16 + S304 
                 Q118 + M248 
                 M105 + I214 
                 W167 + V215 
                 V215 + D337 
                 V291 + H324 
               
               
                 N16 + R320 
                 Q118 + L250 
                 M105 + V215 
                 W167 + L217 
                 V215 + Q345 
                 V291 + F328 
               
               
                 N16 + H321 
                 Q118 + G255 
                 M105 + L217 
                 W167 + L219 
                 V215 + G346 
                 V291 + T334 
               
               
                 N16 + S323 
                 Q118 + Q256 
                 M105 + L219 
                 W167 + L228 
                 V215 + G348 
                 V291 + D337 
               
               
                 N16 + H324 
                 Q118 + A263 
                 M105 + L228 
                 W167 + L235 
                 V215 + T355 
                 V291 + Q345 
               
               
                 N16 + F328 
                 Q118 + V264 
                 M105 + L235 
                 W167 + V238 
                 V215 + S376 
                 V291 + G346 
               
               
                 N16 + T334 
                 Q118 + A265 
                 M105 + V238 
                 W167 + M246 
                 V215 + D377 
                 V291 + G348 
               
               
                 N16 + D337 
                 Q118 + Y267 
                 M105 + M246 
                 W167 + M248 
                 V215 + D379 
                 V291 + T355 
               
               
                 N16 + Q345 
                 Q118 + N270 
                 M105 + M248 
                 W167 + L250 
                 V215 + Y382 
                 V291 + S376 
               
               
                 N16 + G346 
                 Q118 + G273 
                 M105 + L250 
                 W167 + G255 
                 V215 + S383 
                 V291 + D377 
               
               
                 N16 + G348 
                 Q118 + S280 
                 M105 + G255 
                 W167 + Q256 
                 V215 + Q385 
                 V291 + D379 
               
               
                 N16 + T355 
                 Q118 + T285 
                 M105 + Q256 
                 W167 + A263 
                 V215 + K391 
                 V291 + Y382 
               
               
                 N16 + S376 
                 Q118 + M286 
                 M105 + A263 
                 W167 + V264 
                 V215 + K393 
                 V291 + S383 
               
               
                 N16 + D377 
                 Q118 + F289 
                 M105 + V264 
                 W167 + A265 
                 V215 + Q395 
                 V291 + Q385 
               
               
                 N16 + D379 
                 Q118 + V291 
                 M105 + A265 
                 W167 + Y267 
                 L217 + L219 
                 V291 + K391 
               
               
                 N16 + Y382 
                 Q118 + Q299 
                 M105 + Y267 
                 W167 + N270 
                 L217 + L228 
                 V291 + K393 
               
               
                 N16 + S383 
                 Q118 + S304 
                 M105 + N270 
                 W167 + G273 
                 L217 + L235 
                 V291 + Q395 
               
               
                 N16 + Q385 
                 Q118 + R320 
                 M105 + G273 
                 W167 + S280 
                 L217 + V238 
                 Q299 + S304 
               
               
                 N16 + K391 
                 Q118 + H321 
                 M105 + S280 
                 W167 + T285 
                 L217 + M246 
                 Q299 + R320 
               
               
                 N16 + K393 
                 Q118 + S323 
                 M105 + T285 
                 W167 + M286 
                 L217 + M248 
                 Q299 + H321 
               
               
                 N16 + Q395 
                 Q118 + H324 
                 M105 + M286 
                 W167 + F289 
                 L217 + L250 
                 Q299 + S323 
               
               
                 V17 + Q32 
                 Q118 + F328 
                 M105 + F289 
                 W167 + V291 
                 L217 + G255 
                 Q299 + H324 
               
               
                 V17 + A37 
                 Q118 + T334 
                 M105 + V291 
                 W167 + Q299 
                 L217 + Q256 
                 Q299 + F328 
               
               
                 V17 + T40 
                 Q118 + D337 
                 M105 + Q299 
                 W167 + S304 
                 L217 + A263 
                 Q299 + T334 
               
               
                 V17 + P45 
                 Q118 + Q345 
                 M105 + S304 
                 W167 + R320 
                 L217 + V264 
                 Q299 + D337 
               
               
                 V17 + W48 
                 Q118 + G346 
                 M105 + R320 
                 W167 + H321 
                 L217 + A265 
                 Q299 + Q345 
               
               
                 V17 + G50 
                 Q118 + G348 
                 M105 + H321 
                 W167 + S323 
                 L217 + Y267 
                 Q299 + G346 
               
               
                 V17 + T51 
                 Q118 + T355 
                 M105 + S323 
                 W167 + H324 
                 L217 + N270 
                 Q299 + G348 
               
               
                 V17 + N54 
                 Q118 + S376 
                 M105 + H324 
                 W167 + F328 
                 L217 + G273 
                 Q299 + T355 
               
               
                 V17 + V56 
                 Q118 + D377 
                 M105 + F328 
                 W167 + T334 
                 L217 + S280 
                 Q299 + S376 
               
               
                 V17 + A60 
                 Q118 + D379 
                 M105 + T334 
                 W167 + D337 
                 L217 + T285 
                 Q299 + D377 
               
               
                 V17 + K72 
                 Q118 + Y382 
                 M105 + D337 
                 W167 + Q345 
                 L217 + M286 
                 Q299 + D379 
               
               
                 V17 + R87 
                 Q118 + S383 
                 M105 + Q345 
                 W167 + G346 
                 L217 + F289 
                 Q299 + Y382 
               
               
                 V17 + Q98 
                 Q118 + Q385 
                 M105 + G346 
                 W167 + G348 
                 L217 + V291 
                 Q299 + S383 
               
               
                 V17 + M105 
                 Q118 + K391 
                 M105 + G348 
                 W167 + T355 
                 L217 + Q299 
                 Q299 + Q385 
               
               
                 V17 + G109 
                 Q118 + K393 
                 M105 + T355 
                 W167 + S376 
                 L217 + S304 
                 Q299 + K391 
               
               
                 V17 + F113 
                 Q118 + Q395 
                 M105 + S376 
                 W167 + D377 
                 L217 + R320 
                 Q299 + K393 
               
               
                 V17 + R116 
                 Q125 + G133 
                 M105 + D377 
                 W167 + D379 
                 L217 + H321 
                 Q299 + Q395 
               
               
                 V17 + Q118 
                 Q125 + T134 
                 M105 + D379 
                 W167 + Y382 
                 L217 + S323 
                 S304 + R320 
               
               
                 V17 + Q125 
                 Q125 + W140 
                 M105 + Y382 
                 W167 + S383 
                 L217 + H324 
                 S304 + H321 
               
               
                 V17 + G133 
                 Q125 + G142 
                 M105 + S383 
                 W167 + Q385 
                 L217 + F328 
                 S304 + S323 
               
               
                 V17 + T134 
                 Q125 + G149 
                 M105 + Q385 
                 W167 + K391 
                 L217 + T334 
                 S304 + H324 
               
               
                 V17 + W140 
                 Q125 + T165 
                 M105 + K391 
                 W167 + K393 
                 L217 + D337 
                 S304 + F328 
               
               
                 V17 + G142 
                 Q125 + W167 
                 M105 + K393 
                 W167 + Q395 
                 L217 + Q345 
                 S304 + T334 
               
               
                 V17 + G149 
                 Q125 + R171 
                 M105 + Q395 
                 R171 + Q172 
                 L217 + G346 
                 S304 + D337 
               
               
                 V17 + T165 
                 Q125 + Q172 
                 G109 + F113 
                 R171 + L173 
                 L217 + G348 
                 S304 + Q345 
               
               
                 V17 + W167 
                 Q125 + L173 
                 G109 + R116 
                 R171 + A174 
                 L217 + T355 
                 S304 + G346 
               
               
                 V17 + R171 
                 Q125 + A174 
                 G109 + Q118 
                 R171 + G184 
                 L217 + S376 
                 S304 + G348 
               
               
                 V17 + Q172 
                 Q125 + G184 
                 G109 + Q125 
                 R171 + T193 
                 L217 + D377 
                 S304 + T355 
               
               
                 V17 + L173 
                 Q125 + T193 
                 G109 + G133 
                 R171 + N195 
                 L217 + D379 
                 S304 + S376 
               
               
                 V17 + A174 
                 Q125 + N195 
                 G109 + T134 
                 R171 + G196 
                 L217 + Y382 
                 S304 + D377 
               
               
                 V17 + G184 
                 Q125 + G196 
                 G109 + W140 
                 R171 + A204 
                 L217 + S383 
                 S304 + D379 
               
               
                 V17 + T193 
                 Q125 + A204 
                 G109 + G142 
                 R171 + V206 
                 L217 + Q385 
                 S304 + Y382 
               
               
                 V17 + N195 
                 Q125 + V206 
                 G109 + G149 
                 R171 + P211 
                 L217 + K391 
                 S304 + S383 
               
               
                 V17 + G196 
                 Q125 + P211 
                 G109 + T165 
                 R171 + I214 
                 L217 + K393 
                 S304 + Q385 
               
               
                 V17 + A204 
                 Q125 + I214 
                 G109 + W167 
                 R171 + V215 
                 L217 + Q395 
                 S304 + K391 
               
               
                 V17 + V206 
                 Q125 + V215 
                 G109 + R171 
                 R171 + L217 
                 L219 + L228 
                 S304 + K393 
               
               
                 V17 + P211 
                 Q125 + L217 
                 G109 + Q172 
                 R171 + L219 
                 L219 + L235 
                 S304 + Q395 
               
               
                 V17 + I214 
                 Q125 + L219 
                 G109 + L173 
                 R171 + L228 
                 L219 + V238 
                 R320 + H321 
               
               
                 V17 + V215 
                 Q125 + L228 
                 G109 + A174 
                 R171 + L235 
                 L219 + M246 
                 R320 + S323 
               
               
                 V17 + L217 
                 Q125 + L235 
                 G109 + G184 
                 R171 + V238 
                 L219 + M248 
                 R320 + H324 
               
               
                 V17 + L219 
                 Q125 + V238 
                 G109 + T193 
                 R171 + M246 
                 L219 + L250 
                 R320 + F328 
               
               
                 V17 + L228 
                 Q125 + M246 
                 G109 + N195 
                 R171 + M248 
                 L219 + G255 
                 R320 + T334 
               
               
                 V17 + L235 
                 Q125 + M248 
                 G109 + G196 
                 R171 + L250 
                 L219 + Q256 
                 R320 + D337 
               
               
                 V17 + V238 
                 Q125 + L250 
                 G109 + A204 
                 R171 + G255 
                 L219 + A263 
                 R320 + Q345 
               
               
                 V17 + M246 
                 A37 + Q395 
                 G109 + V206 
                 R171 + Q256 
                 L219 + V264 
                 R320 + G346 
               
               
                 V17 + M248 
                 T40 + P45 
                 G109 + P211 
                 R171 + A263 
                 L219 + A265 
                 R320 + G348 
               
               
                 V17 + L250 
                 T40 + W48 
                 G109 + I214 
                 R171 + V264 
                 L219 + Y267 
                 R320 + T355 
               
               
                 V17 + G255 
                 T40 + G50 
                 G109 + V215 
                 R171 + A265 
                 L219 + N270 
                 R320 + S376 
               
               
                 V17 + Q256 
                 T40 + T51 
                 G109 + L217 
                 R171 + Y267 
                 L219 + G273 
                 R320 + D377 
               
               
                 V17 + A263 
                 T40 + N54 
                 G109 + L219 
                 R171 + N270 
                 L219 + S280 
                 R320 + D379 
               
               
                 V17 + V264 
                 T40 + V56 
                 G109 + L228 
                 R171 + G273 
                 L219 + T285 
                 R320 + Y382 
               
               
                 V17 + A265 
                 T40 + A60 
                 G109 + L235 
                 R171 + S280 
                 L219 + M286 
                 R320 + S383 
               
               
                 V17 + Y267 
                 T40 + K72 
                 G109 + V238 
                 R171 + T285 
                 L219 + F289 
                 R320 + Q385 
               
               
                 V17 + N270 
                 T40 + R87 
                 G109 + M246 
                 R171 + M286 
                 L219 + V291 
                 R320 + K391 
               
               
                 V17 + G273 
                 T40 + Q98 
                 G109 + M248 
                 R171 + F289 
                 L219 + Q299 
                 R320 + K393 
               
               
                 V17 + S280 
                 T40 + M105 
                 G109 + L250 
                 R171 + V291 
                 L219 + S304 
                 R320 + Q395 
               
               
                 V17 + T285 
                 T40 + G109 
                 G109 + G255 
                 R171 + Q299 
                 L219 + R320 
                 H321 + S323 
               
               
                 V17 + M286 
                 T40 + F113 
                 G109 + Q256 
                 R171 + S304 
                 L219 + H321 
                 H321 + H324 
               
               
                 V17 + F289 
                 T40 + R116 
                 G109 + A263 
                 R171 + R320 
                 L219 + S323 
                 H321 + F328 
               
               
                 V17 + V291 
                 T40 + Q118 
                 G109 + V264 
                 R171 + H321 
                 L219 + H324 
                 H321 + T334 
               
               
                 V17 + Q299 
                 T40 + Q125 
                 G109 + A265 
                 R171 + S323 
                 L219 + F328 
                 H321 + D337 
               
               
                 V17 + S304 
                 T40 + G133 
                 G109 + Y267 
                 R171 + H324 
                 L219 + T334 
                 H321 + Q345 
               
               
                 V17 + R320 
                 T40 + T134 
                 G109 + N270 
                 R171 + F328 
                 L219 + D337 
                 H321 + G346 
               
               
                 V17 + H321 
                 T40 + W140 
                 G109 + G273 
                 R171 + T334 
                 L219 + Q345 
                 H321 + G348 
               
               
                 V17 + S323 
                 T40 + G142 
                 G109 + S280 
                 R171 + D337 
                 L219 + G346 
                 H321 + T355 
               
               
                 V17 + H324 
                 T40 + G149 
                 G109 + T285 
                 R171 + Q345 
                 L219 + G348 
                 H321 + S376 
               
               
                 V17 + F328 
                 T40 + T165 
                 G109 + M286 
                 R171 + G346 
                 L219 + T355 
                 H321 + D377 
               
               
                 V17 + T334 
                 T40 + W167 
                 G109 + F289 
                 R171 + G348 
                 L219 + S376 
                 H321 + D379 
               
               
                 V17 + D337 
                 T40 + R171 
                 G109 + V291 
                 R171 + T355 
                 L219 + D377 
                 H321 + Y382 
               
               
                 V17 + Q345 
                 T40 + Q172 
                 G109 + Q299 
                 R171 + S376 
                 L219 + D379 
                 H321 + S383 
               
               
                 V17 + G346 
                 T40 + L173 
                 G109 + S304 
                 R171 + D377 
                 L219 + Y382 
                 H321 + Q385 
               
               
                 V17 + G348 
                 T40 + A174 
                 G109 + R320 
                 R171 + D379 
                 L219 + S383 
                 H321 + K391 
               
               
                 V17 + T355 
                 T40 + G184 
                 G109 + H321 
                 R171 + Y382 
                 L219 + Q385 
                 H321 + K393 
               
               
                 V17 + S376 
                 T40 + T193 
                 G109 + S323 
                 R171 + S383 
                 L219 + K391 
                 H321 + Q395 
               
               
                 V17 + D377 
                 T40 + N195 
                 G109 + H324 
                 R171 + Q385 
                 L219 + K393 
                 S323 + H324 
               
               
                 V17 + D379 
                 T40 + G196 
                 G109 + F328 
                 R171 + K391 
                 L219 + Q395 
                 S323 + F328 
               
               
                 V17 + Y382 
                 T40 + A204 
                 G109 + T334 
                 R171 + K393 
                 L228 + L235 
                 S323 + T334 
               
               
                 V17 + S383 
                 T40 + V206 
                 G109 + D337 
                 R171 + Q395 
                 L228 + V238 
                 S323 + D337 
               
               
                 V17 + Q385 
                 T40 + P211 
                 G109 + Q345 
                 Q172 + L173 
                 L228 + M246 
                 S323 + Q345 
               
               
                 V17 + K391 
                 T40 + I214 
                 G109 + G346 
                 Q172 + A174 
                 L228 + M248 
                 S323 + G346 
               
               
                 V17 + K393 
                 T40 + V215 
                 G109 + G348 
                 Q172 + G184 
                 L228 + L250 
                 S323 + G348 
               
               
                 V17 + Q395 
                 H1 + Q169 
                 G109 + T355 
                 Q172 + T193 
                 L228 + G255 
                 S323 + T355 
               
               
                 Q32 + A37 
                 H1 + A186 
                 G109 + S376 
                 Q172 + N195 
                 L228 + Q256 
                 S323 + S376 
               
               
                 Q32 + T40 
                 H1 + E190 
                 G109 + D377 
                 Q172 + G196 
                 L228 + A263 
                 S323 + D377 
               
               
                 Q32 + P45 
                 H1 + A225 
                 G109 + D379 
                 Q172 + A204 
                 L228 + V264 
                 S323 + D379 
               
               
                 Q32 + W48 
                 H1 + K242 
                 G109 + Y382 
                 Q172 + V206 
                 L228 + A265 
                 S323 + Y382 
               
               
                 Q32 + G50 
                 H1 + S244 
                 G109 + S383 
                 Q172 + P211 
                 L228 + Y267 
                 S323 + S383 
               
               
                 Q32 + T51 
                 H1 + N260 
                 G109 + Q385 
                 Q172 + I214 
                 L228 + N270 
                 S323 + Q385 
               
               
                 Q32 + N54 
                 H1 + K269 
                 G109 + K391 
                 Q172 + V215 
                 L228 + G273 
                 S323 + K391 
               
               
                 Q32 + V56 
                 H1 + W284 
                 G109 + K393 
                 Q172 + L217 
                 L228 + S280 
                 S323 + K393 
               
               
                 Q32 + A60 
                 H1 + Y295 
                 G109 + Q395 
                 Q172 + L219 
                 L228 + T285 
                 S323 + Q395 
               
               
                 Q32 + K72 
                 H1 + V326 
                 F113 + R116 
                 Q172 + L228 
                 L228 + M286 
                 H324 + F328 
               
               
                 Q32 + R87 
                 T5 + Q169 
                 F113 + Q118 
                 Q172 + L235 
                 L228 + F289 
                 H324 + T334 
               
               
                 Q32 + Q98 
                 T5 + A186 
                 F113 + Q125 
                 Q172 + V238 
                 L228 + V291 
                 H324 + D337 
               
               
                 Q32 + M105 
                 T5 + E190 
                 F113 + G133 
                 Q172 + M246 
                 L228 + Q299 
                 H324 + Q345 
               
               
                 Q32 + G109 
                 T5 + A225 
                 F113 + T134 
                 Q172 + M248 
                 L228 + S304 
                 H324 + G346 
               
               
                 Q32 + F113 
                 T5 + K242 
                 F113 + W140 
                 Q172 + L250 
                 L228 + R320 
                 H324 + G348 
               
               
                 Q32 + R116 
                 T5 + S244 
                 F113 + G142 
                 Q172 + G255 
                 L228 + H321 
                 H324 + T355 
               
               
                 Q32 + Q118 
                 T5 + N260 
                 F113 + G149 
                 Q172 + Q256 
                 L228 + S323 
                 H324 + S376 
               
               
                 Q32 + Q125 
                 T5 + K269 
                 F113 + T165 
                 Q172 + A263 
                 L228 + H324 
                 H324 + D377 
               
               
                 Q32 + G133 
                 T5 + W284 
                 F113 + W167 
                 Q172 + V264 
                 L228 + F328 
                 H324 + D379 
               
               
                 Q32 + T134 
                 T5 + Y295 
                 F113 + R171 
                 Q172 + A265 
                 L228 + T334 
                 H324 + Y382 
               
               
                 Q32 + W140 
                 T5 + V326 
                 F113 + Q172 
                 Q172 + Y267 
                 L228 + D337 
                 H324 + S383 
               
               
                 Q32 + G142 
                 G7 + Q169 
                 F113 + L173 
                 Q172 + N270 
                 L228 + Q345 
                 H324 + Q385 
               
               
                 Q32 + G149 
                 G7 + A186 
                 F113 + A174 
                 Q172 + G273 
                 L228 + G346 
                 H324 + K391 
               
               
                 Q32 + T165 
                 G7 + E190 
                 F113 + G184 
                 Q172 + S280 
                 L228 + G348 
                 H324 + K393 
               
               
                 Q32 + W167 
                 G7 + A225 
                 F113 + T193 
                 Q172 + T285 
                 L228 + T355 
                 H324 + Q395 
               
               
                 Q32 + R171 
                 G7 + K242 
                 F113 + N195 
                 Q172 + M286 
                 L228 + S376 
                 F328 + T334 
               
               
                 Q32 + Q172 
                 G7 + S244 
                 F113 + G196 
                 Q172 + F289 
                 L228 + D377 
                 F328 + D337 
               
               
                 Q32 + L173 
                 G7 + N260 
                 F113 + A204 
                 Q172 + V291 
                 L228 + D379 
                 F328 + Q345 
               
               
                 Q32 + A174 
                 G7 + K269 
                 F113 + V206 
                 Q172 + Q299 
                 L228 + Y382 
                 F328 + G346 
               
               
                 Q32 + G184 
                 G7 + W284 
                 F113 + P211 
                 Q172 + S304 
                 L228 + S383 
                 F328 + G348 
               
               
                 Q32 + T193 
                 G7 + Y295 
                 F113 + I214 
                 Q172 + R320 
                 L228 + Q385 
                 F328 + T355 
               
               
                 Q32 + N195 
                 G7 + V326 
                 F113 + V215 
                 Q172 + H321 
                 L228 + K391 
                 F328 + S376 
               
               
                 Q32 + G196 
                 Q11 + Q169 
                 F113 + L217 
                 Q172 + S323 
                 L228 + K393 
                 F328 + D377 
               
               
                 Q32 + A204 
                 Q11 + A186 
                 F113 + L219 
                 Q172 + H324 
                 L228 + Q395 
                 F328 + D379 
               
               
                 Q32 + V206 
                 Q11 + E190 
                 F113 + L228 
                 Q172 + F328 
                 L235 + V238 
                 F328 + Y382 
               
               
                 Q32 + P211 
                 Q11 + A225 
                 F113 + L235 
                 Q172 + T334 
                 L235 + M246 
                 F328 + S383 
               
               
                 Q32 + I214 
                 Q11 + K242 
                 F113 + V238 
                 Q172 + D337 
                 L235 + M248 
                 F328 + Q385 
               
               
                 Q32 + V215 
                 Q11 + S244 
                 F113 + M246 
                 Q172 + Q345 
                 L235 + L250 
                 F328 + K391 
               
               
                 Q32 + L217 
                 Q11 + N260 
                 F113 + M248 
                 Q172 + G346 
                 L235 + G255 
                 F328 + K393 
               
               
                 Q32 + L219 
                 Q11 + K269 
                 F113 + L250 
                 Q172 + G348 
                 L235 + Q256 
                 F328 + Q395 
               
               
                 Q32 + L228 
                 Q11 + W284 
                 F113 + G255 
                 Q172 + T355 
                 L235 + A263 
                 T334 + D337 
               
               
                 Q32 + L235 
                 Q11 + Y295 
                 F113 + Q256 
                 Q172 + S376 
                 L235 + V264 
                 T334 + Q345 
               
               
                 Q32 + V238 
                 Q11 + V326 
                 F113 + A263 
                 Q172 + D377 
                 L235 + A265 
                 T334 + G346 
               
               
                 Q32 + M246 
                 N16 + Q169 
                 F113 + V264 
                 Q172 + D379 
                 L235 + Y267 
                 T334 + G348 
               
               
                 Q32 + M248 
                 N16 + A186 
                 F113 + A265 
                 Q172 + Y382 
                 L235 + N270 
                 T334 + T355 
               
               
                 Q32 + L250 
                 N16 + E190 
                 F113 + Y267 
                 Q172 + S383 
                 L235 + G273 
                 T334 + S376 
               
               
                 Q32 + G255 
                 N16 + A225 
                 F113 + N270 
                 Q172 + Q385 
                 L235 + S280 
                 T334 + D377 
               
               
                 Q32 + Q256 
                 N16 + K242 
                 F113 + G273 
                 Q172 + K391 
                 L235 + T285 
                 T334 + D379 
               
               
                 Q32 + A263 
                 N16 + S244 
                 F113 + S280 
                 Q172 + K393 
                 L235 + M286 
                 T334 + Y382 
               
               
                 Q32 + V264 
                 N16 + N260 
                 F113 + T285 
                 Q172 + Q395 
                 L235 + F289 
                 T334 + S383 
               
               
                 Q32 + A265 
                 N16 + K269 
                 F113 + M286 
                 L173 + A174 
                 L235 + V291 
                 T334 + Q385 
               
               
                 Q32 + Y267 
                 N16 + W284 
                 F113 + F289 
                 L173 + G184 
                 L235 + Q299 
                 T334 + K391 
               
               
                 Q32 + N270 
                 N16 + Y295 
                 F113 + V291 
                 L173 + T193 
                 L235 + S304 
                 T334 + K393 
               
               
                 Q32 + G273 
                 N16 + V326 
                 F113 + Q299 
                 L173 + N195 
                 L235 + R320 
                 T334 + Q395 
               
               
                 Q32 + S280 
                 V17 + Q169 
                 F113 + S304 
                 L173 + G196 
                 L235 + H321 
                 D337 + Q345 
               
               
                 Q32 + T285 
                 V17 + A186 
                 F113 + R320 
                 L173 + A204 
                 L235 + S323 
                 D337 + G346 
               
               
                 Q32 + M286 
                 V17 + E190 
                 F113 + H321 
                 L173 + V206 
                 L235 + H324 
                 D337 + G348 
               
               
                 Q32 + F289 
                 V17 + A225 
                 F113 + S323 
                 L173 + P211 
                 L235 + F328 
                 D337 + T355 
               
               
                 Q32 + V291 
                 V17 + K242 
                 F113 + H324 
                 L173 + I214 
                 L235 + T334 
                 D337 + S376 
               
               
                 Q32 + Q299 
                 V17 + S244 
                 F113 + F328 
                 L173 + V215 
                 L235 + D337 
                 D337 + D377 
               
               
                 Q32 + S304 
                 V17 + N260 
                 F113 + T334 
                 L173 + L217 
                 L235 + Q345 
                 D337 + D379 
               
               
                 Q32 + R320 
                 V17 + K269 
                 F113 + D337 
                 L173 + L219 
                 L235 + G346 
                 D337 + Y382 
               
               
                 Q32 + H321 
                 V17 + W284 
                 F113 + Q345 
                 L173 + L228 
                 L235 + G348 
                 D337 + S383 
               
               
                 Q32 + S323 
                 V17 + Y295 
                 F113 + G346 
                 L173 + L235 
                 L235 + T355 
                 D337 + Q385 
               
               
                 Q32 + H324 
                 V17 + V326 
                 F113 + G348 
                 L173 + V238 
                 L235 + S376 
                 D337 + K391 
               
               
                 Q32 + F328 
                 Q32 + Q169 
                 F113 + T355 
                 L173 + M246 
                 L235 + D377 
                 D337 + K393 
               
               
                 Q32 + T334 
                 Q32 + A186 
                 F113 + S376 
                 L173 + M248 
                 L235 + D379 
                 D337 + Q395 
               
               
                 Q32 + D337 
                 Q32 + E190 
                 F113 + D377 
                 L173 + L250 
                 L235 + Y382 
                 Q345 + G346 
               
               
                 Q32 + Q345 
                 Q32 + A225 
                 F113 + D379 
                 L173 + G255 
                 L235 + S383 
                 Q345 + G348 
               
               
                 Q32 + G346 
                 Q32 + K242 
                 F113 + Y382 
                 L173 + Q256 
                 L235 + Q385 
                 Q345 + T355 
               
               
                 Q32 + G348 
                 Q32 + S244 
                 F113 + S383 
                 L173 + A263 
                 L235 + K391 
                 Q345 + S376 
               
               
                 Q32 + T355 
                 Q32 + N260 
                 F113 + Q385 
                 L173 + V264 
                 L235 + K393 
                 Q345 + D377 
               
               
                 Q32 + S376 
                 Q32 + K269 
                 F113 + K391 
                 L173 + A265 
                 L235 + Q395 
                 Q345 + D379 
               
               
                 Q32 + D377 
                 Q32 + W284 
                 F113 + K393 
                 L173 + Y267 
                 V238 + M246 
                 Q345 + Y382 
               
               
                 Q32 + D379 
                 Q32 + Y295 
                 F113 + Q395 
                 L173 + N270 
                 V238 + M248 
                 Q345 + S383 
               
               
                 Q32 + Y382 
                 Q32 + V326 
                 R116 + Q118 
                 L173 + G273 
                 V238 + L250 
                 Q345 + Q385 
               
               
                 Q32 + S383 
                 A37 + Q169 
                 R116 + Q125 
                 L173 + S280 
                 V238 + G255 
                 Q345 + K391 
               
               
                 Q32 + Q385 
                 A37 + A186 
                 R116 + G133 
                 L173 + T285 
                 V238 + Q256 
                 Q345 + K393 
               
               
                 Q32 + K391 
                 A37 + E190 
                 R116 + T134 
                 L173 + M286 
                 V238 + A263 
                 Q345 + Q395 
               
               
                 Q32 + K393 
                 A37 + A225 
                 R116 + W140 
                 L173 + F289 
                 V238 + V264 
                 G346 + G348 
               
               
                 Q32 + Q395 
                 A37 + K242 
                 R116 + G142 
                 L173 + V291 
                 V238 + A265 
                 G346 + T355 
               
               
                 A37 + T40 
                 A37 + S244 
                 R116 + G149 
                 L173 + Q299 
                 V238 + Y267 
                 G346 + S376 
               
               
                 A37 + P45 
                 A37 + N260 
                 R116 + T165 
                 L173 + S304 
                 V238 + N270 
                 G346 + D377 
               
               
                 A37 + W48 
                 A37 + K269 
                 R116 + W167 
                 L173 + R320 
                 V238 + G273 
                 G346 + D379 
               
               
                 A37 + G50 
                 A37 + W284 
                 R116 + R171 
                 L173 + H321 
                 V238 + S280 
                 G346 + Y382 
               
               
                 A37 + T51 
                 A37 + Y295 
                 R116 + Q172 
                 L173 + S323 
                 V238 + T285 
                 G346 + S383 
               
               
                 A37 + N54 
                 A37 + V326 
                 R116 + L173 
                 L173 + H324 
                 V238 + M286 
                 G346 + Q385 
               
               
                 A37 + V56 
                 T40 + Q169 
                 R116 + A174 
                 L173 + F328 
                 V238 + F289 
                 G346 + K391 
               
               
                 A37 + A60 
                 T40 + A186 
                 R116 + G184 
                 L173 + T334 
                 V238 + V291 
                 G346 + K393 
               
               
                 A37 + K72 
                 T40 + E190 
                 R116 + T193 
                 L173 + D337 
                 V238 + Q299 
                 G346 + Q395 
               
               
                 A37 + R87 
                 T40 + A225 
                 R116 + N195 
                 L173 + Q345 
                 V238 + S304 
                 G348 + T355 
               
               
                 A37 + Q98 
                 T40 + K242 
                 R116 + G196 
                 L173 + G346 
                 V238 + R320 
                 G348 + S376 
               
               
                 A37 + M105 
                 T40 + S244 
                 R116 + A204 
                 L173 + G348 
                 V238 + H321 
                 G348 + D377 
               
               
                 A37 + G109 
                 T40 + N260 
                 R116 + V206 
                 L173 + T355 
                 V238 + S323 
                 G348 + D379 
               
               
                 A37 + F113 
                 T40 + K269 
                 R116 + P211 
                 L173 + S376 
                 V238 + H324 
                 G348 + Y382 
               
               
                 A37 + R116 
                 T40 + W284 
                 R116 + I214 
                 L173 + D377 
                 V238 + F328 
                 G348 + S383 
               
               
                 A37 + Q118 
                 T40 + Y295 
                 R116 + V215 
                 L173 + D379 
                 V238 + T334 
                 G348 + Q385 
               
               
                 A37 + Q125 
                 T40 + V326 
                 R116 + L217 
                 L173 + Y382 
                 V238 + D337 
                 G348 + K391 
               
               
                 A37 + G133 
                 P45 + Q169 
                 R116 + L219 
                 L173 + S383 
                 V238 + Q345 
                 G348 + K393 
               
               
                 A37 + T134 
                 P45 + A186 
                 R116 + L228 
                 L173 + Q385 
                 V238 + G346 
                 G348 + Q395 
               
               
                 A37 + W140 
                 P45 + E190 
                 R116 + L235 
                 L173 + K391 
                 V238 + G348 
                 T355 + S376 
               
               
                 A37 + G142 
                 P45 + A225 
                 R116 + V238 
                 L173 + K393 
                 V238 + T355 
                 T355 + D377 
               
               
                 A37 + G149 
                 P45 + K242 
                 R116 + M246 
                 L173 + Q395 
                 V238 + S376 
                 T355 + D379 
               
               
                 A37 + T165 
                 P45 + S244 
                 R116 + M248 
                 A174 + G184 
                 V238 + D377 
                 T355 + Y382 
               
               
                 A37 + W167 
                 P45 + N260 
                 R116 + L250 
                 A174 + T193 
                 V238 + D379 
                 T355 + S383 
               
               
                 A37 + R171 
                 P45 + K269 
                 R116 + G255 
                 A174 + N195 
                 V238 + Y382 
                 T355 + Q385 
               
               
                 A37 + Q172 
                 P45 + W284 
                 R116 + Q256 
                 A174 + G196 
                 V238 + S383 
                 T355 + K391 
               
               
                 A37 + L173 
                 P45 + Y295 
                 R116 + A263 
                 A174 + A204 
                 V238 + Q385 
                 T355 + K393 
               
               
                 A37 + A174 
                 P45 + V326 
                 R116 + V264 
                 A174 + V206 
                 V238 + K391 
                 T355 + Q395 
               
               
                 A37 + G184 
                 W48 + Q169 
                 R116 + A265 
                 A174 + P211 
                 V238 + K393 
                 S376 + D377 
               
               
                 A37 + T193 
                 W48 + A186 
                 R116 + Y267 
                 A174 + I214 
                 V238 + Q395 
                 S376 + D379 
               
               
                 A37 + N195 
                 W48 + E190 
                 R116 + N270 
                 A174 + V215 
                 M246 + M248 
                 S376 + Y382 
               
               
                 A37 + G196 
                 W48 + A225 
                 R116 + G273 
                 A174 + L217 
                 M246 + L250 
                 S376 + S383 
               
               
                 A37 + A204 
                 W48 + K242 
                 R116 + S280 
                 A174 + L219 
                 M246 + G255 
                 S376 + Q385 
               
               
                 A37 + V206 
                 W48 + S244 
                 R116 + T285 
                 A174 + L228 
                 M246 + Q256 
                 S376 + K391 
               
               
                 A37 + P211 
                 W48 + N260 
                 R116 + M286 
                 A174 + L235 
                 M246 + A263 
                 S376 + K393 
               
               
                 A37 + I214 
                 W48 + K269 
                 R116 + F289 
                 A174 + V238 
                 M246 + V264 
                 S376 + Q395 
               
               
                 A37 + V215 
                 W48 + W284 
                 R116 + V291 
                 A174 + M246 
                 M246 + A265 
                 D377 + D379 
               
               
                 A37 + L217 
                 W48 + Y295 
                 R116 + Q299 
                 A174 + M248 
                 M246 + Y267 
                 D377 + Y382 
               
               
                 A37 + L219 
                 W48 + V326 
                 R116 + S304 
                 A174 + L250 
                 M246 + N270 
                 D377 + S383 
               
               
                 A37 + L228 
                 G50 + Q169 
                 R116 + R320 
                 A174 + G255 
                 M246 + G273 
                 D377 + Q385 
               
               
                 A37 + L235 
                 G50 + A186 
                 R116 + H321 
                 A174 + Q256 
                 M246 + S280 
                 D377 + K391 
               
               
                 A37 + V238 
                 G50 + E190 
                 R116 + S323 
                 A174 + A263 
                 M246 + T285 
                 D377 + K393 
               
               
                 A37 + M246 
                 G50 + A225 
                 R116 + H324 
                 A174 + V264 
                 M246 + M286 
                 D377 + Q395 
               
               
                 A37 + M248 
                 G50 + K242 
                 R116 + F328 
                 A174 + A265 
                 M246 + F289 
                 D379 + Y382 
               
               
                 A37 + L250 
                 G50 + S244 
                 R116 + T334 
                 A174 + Y267 
                 M246 + V291 
                 D379 + S383 
               
               
                 A37 + G255 
                 G50 + N260 
                 R116 + D337 
                 A174 + N270 
                 M246 + Q299 
                 D379 + Q385 
               
               
                 A37 + Q256 
                 G50 + K269 
                 R116 + Q345 
                 A174 + G273 
                 M246 + S304 
                 D379 + K391 
               
               
                 A37 + A263 
                 G50 + W284 
                 R116 + G346 
                 A174 + S280 
                 M246 + R320 
                 D379 + K393 
               
               
                 A37 + V264 
                 G50 + Y295 
                 R116 + G348 
                 A174 + T285 
                 M246 + H321 
                 D379 + Q395 
               
               
                 A37 + A265 
                 G50 + V326 
                 R116 + T355 
                 A174 + M286 
                 M246 + S323 
                 Y382 + S383 
               
               
                 A37 + Y267 
                 T51 + Q169 
                 R116 + S376 
                 A174 + F289 
                 M246 + H324 
                 Y382 + Q385 
               
               
                 A37 + N270 
                 T51 + A186 
                 R116 + D377 
                 A174 + V291 
                 M246 + F328 
                 Y382 + K391 
               
               
                 A37 + G273 
                 T51 + E190 
                 R116 + D379 
                 A174 + Q299 
                 M246 + T334 
                 Y382 + K393 
               
               
                 A37 + S280 
                 T51 + A225 
                 R116 + Y382 
                 A174 + S304 
                 M246 + D337 
                 Y382 + Q395 
               
               
                 A37 + T285 
                 T51 + K242 
                 R116 + S383 
                 A174 + R320 
                 M246 + Q345 
                 S383 + Q385 
               
               
                 A37 + M286 
                 T51 + S244 
                 R116 + Q385 
                 A174 + H321 
                 M246 + G346 
                 S383 + K391 
               
               
                 A37 + F289 
                 T51 + N260 
                 R116 + K391 
                 A174 + S323 
                 M246 + G348 
                 S383 + K393 
               
               
                 A37 + V291 
                 T51 + K269 
                 R116 + K393 
                 A174 + H324 
                 M246 + T355 
                 S383 + Q395 
               
               
                 A37 + Q299 
                 T51 + W284 
                 R116 + Q395 
                 A174 + F328 
                 M246 + S376 
                 Q385 + K391 
               
               
                 A37 + S304 
                 T51 + Y295 
                 Q118 + Q125 
                 A174 + T334 
                 M246 + D377 
                 Q385 + K393 
               
               
                 A37 + R320 
                 T51 + V326 
                 Q118 + G133 
                 A174 + D337 
                 M246 + D379 
                 Q385 + Q395 
               
               
                 A37 + H321 
                 N54 + Q169 
                 Q118 + T134 
                 A174 + Q345 
                 M246 + Y382 
                 K391 + K393 
               
               
                 A37 + S323 
                 N54 + A186 
                 Q118 + W140 
                 A174 + G346 
                 M246 + S383 
                 K391 + Q395 
               
               
                 A37 + H324 
                 N54 + E190 
                 Q118 + G142 
                 A174 + G348 
                 M246 + Q385 
                 K393 + Q395 
               
               
                 A37 + F328 
                 N54 + A225 
                 Q118 + G149 
                 A174 + T355 
                 M246 + K391 
                 M248 + F289 
               
               
                 A37 + T334 
                 N54 + K242 
                 Q118 + T165 
                 A174 + S376 
                 M246 + K393 
                 M248 + V291 
               
               
                 A37 + D337 
                 N54 + S244 
                 Q118 + W167 
                 A174 + D377 
                 M246 + Q395 
                 G109 + Q169 
               
               
                 A37 + Q345 
                 N54 + N260 
                 Q118 + R171 
                 A174 + D379 
                 M248 + L250 
                 G109 + A186 
               
               
                 A37 + G346 
                 N54 + K269 
                 Q118 + Q172 
                 A174 + Y382 
                 M248 + G255 
                 G109 + E190 
               
               
                 A37 + G348 
                 N54 + W284 
                 Q118 + L173 
                 A174 + S383 
                 M248 + Q256 
                 G109 + A225 
               
               
                 A37 + T355 
                 N54 + Y295 
                 Q118 + A174 
                 A174 + Q385 
                 M248 + A263 
                 G109 + K242 
               
               
                 A37 + S376 
                 N54 + V326 
                 Q118 + G184 
                 A174 + K391 
                 M248 + V264 
                 G109 + S244 
               
               
                 A37 + D377 
                 V56 + Q169 
                 Q118 + T193 
                 A174 + K393 
                 M248 + A265 
                 G109 + N260 
               
               
                 A37 + D379 
                 V56 + A186 
                 Q118 + N195 
                 A174 + Q395 
                 M248 + Y267 
                 G109 + K269 
               
               
                 A37 + Y382 
                 V56 + E190 
                 Q118 + G196 
                 G184 + T193 
                 M248 + N270 
                 G109 + W284 
               
               
                 A37 + S383 
                 V56 + A225 
                 Q118 + A204 
                 G184 + N195 
                 M248 + G273 
                 G109 + Y295 
               
               
                 A37 + Q385 
                 V56 + K242 
                 Q118 + V206 
                 G184 + G196 
                 M248 + S280 
                 G109 + V326 
               
               
                 A37 + K391 
                 V56 + S244 
                 Q118 + P211 
                 G184 + A204 
                 M248 + T285 
                 F113 + Q169 
               
               
                 A37 + K393 
                 V56 + N260 
                 Q118 + I214 
                 G184 + V206 
                 M248 + M286 
                 F113 + A186 
               
               
                 A60 + Q169 
                 V56 + K269 
                 G133 + Q169 
                 W167 + Q169 
                 R171 + A186 
                 F113 + A225 
               
               
                 A60 + A186 
                 V56 + W284 
                 G133 + A186 
                 W167 + A186 
                 R171 + E190 
                 F113 + E190 
               
               
                 A60 + E190 
                 V56 + Y295 
                 G133 + E190 
                 W167 + E190 
                 R171 + A225 
                 F113 + K242 
               
               
                 A60 + A225 
                 V56 + V326 
                 G133 + A225 
                 W167 + A225 
                 R171 + K242 
                 F113 + S244 
               
               
                 A60 + K242 
                 G184 + A186 
                 G133 + K242 
                 W167 + K242 
                 R171 + S244 
                 F113 + N260 
               
               
                 A60 + S244 
                 G184 + E190 
                 G133 + S244 
                 W167 + S244 
                 R171 + N260 
                 F113 + K269 
               
               
                 A60 + N260 
                 G184 + A225 
                 G133 + N260 
                 W167 + N260 
                 R171 + K269 
                 F113 + W284 
               
               
                 A60 + K269 
                 G184 + K242 
                 G133 + K269 
                 W167 + K269 
                 R171 + W284 
                 F113 + Y295 
               
               
                 A60 + W284 
                 G184 + S244 
                 G133 + W284 
                 W167 + W284 
                 R171 + Y295 
                 F113 + V326 
               
               
                 A60 + Y295 
                 G184 + N260 
                 G133 + Y295 
                 W167 + Y295 
                 R171 + V326 
                 R116 + Q169 
               
               
                 A60 + V326 
                 G184 + K269 
                 G133 + V326 
                 W167 + V326 
                 Q172 + A186 
                 R116 + A186 
               
               
                 K72 + Q169 
                 G184 + W284 
                 T134 + Q169 
                 Q169 + A186 
                 Q172 + E190 
                 R116 + E190 
               
               
                 K72 + A186 
                 G184 + Y295 
                 T134 + A186 
                 Q169 + E190 
                 Q172 + A225 
                 R116 + A225 
               
               
                 K72 + E190 
                 G184 + V326 
                 T134 + E190 
                 Q169 + L173 
                 Q172 + K242 
                 R116 + K242 
               
               
                 K72 + A225 
                 A186 + E190 
                 T134 + A225 
                 Q169 + A174 
                 Q172 + S244 
                 R116 + S244 
               
               
                 K72 + K242 
                 A186 + T193 
                 T134 + K242 
                 Q169 + G184 
                 Q172 + N260 
                 R116 + N260 
               
               
                 K72 + S244 
                 A186 + N195 
                 T134 + S244 
                 Q169 + T193 
                 Q172 + K269 
                 R116 + K269 
               
               
                 K72 + N260 
                 A186 + G196 
                 T134 + N260 
                 Q169 + N195 
                 Q172 + W284 
                 R116 + W284 
               
               
                 K72 + K269 
                 A186 + A204 
                 T134 + K269 
                 Q169 + G196 
                 Q172 + Y295 
                 R116 + Y295 
               
               
                 K72 + W284 
                 A186 + V206 
                 T134 + W284 
                 Q169 + A204 
                 Q172 + V326 
                 R116 + V326 
               
               
                 K72 + Y295 
                 A186 + P211 
                 T134 + Y295 
                 Q169 + V206 
                 L173 + A186 
                 Q118 + Q169 
               
               
                 K72 + V326 
                 A186 + I214 
                 T134 + V326 
                 Q169 + P211 
                 L173 + E190 
                 Q118 + A186 
               
               
                 R87 + Q169 
                 A186 + V215 
                 W140 + Q169 
                 Q169 + I214 
                 L173 + A225 
                 Q118 + A225 
               
               
                 R87 + A186 
                 A186 + L217 
                 W140 + A186 
                 Q169 + V215 
                 L173 + K242 
                 Q118 + K242 
               
               
                 R87 + E190 
                 A186 + L219 
                 W140 + E190 
                 Q169 + L217 
                 L173 + S244 
                 Q118 + S244 
               
               
                 R87 + A225 
                 A186 + A225 
                 W140 + A225 
                 Q169 + L219 
                 L173 + N260 
                 Q118 + N260 
               
               
                 R87 + K242 
                 A186 + L228 
                 W140 + K242 
                 Q169 + A225 
                 L173 + K269 
                 Q118 + K269 
               
               
                 R87 + S244 
                 A186 + L235 
                 W140 + S244 
                 Q169 + L228 
                 L173 + W284 
                 Q118 + W284 
               
               
                 R87 + N260 
                 A186 + V238 
                 W140 + N260 
                 Q169 + L235 
                 L173 + Y295 
                 Q118 + Y295 
               
               
                 R87 + K269 
                 A186 + K242 
                 W140 + K269 
                 Q169 + V238 
                 L173 + V326 
                 Q118 + V326 
               
               
                 R87 + W284 
                 A186 + S244 
                 W140 + W284 
                 Q169 + K242 
                 A174 + A186 
                 Q125 + Q169 
               
               
                 R87 + Y295 
                 A186 + M246 
                 W140 + Y295 
                 Q169 + S244 
                 A174 + E190 
                 Q125 + A186 
               
               
                 R87 + V326 
                 A186 + M248 
                 W140 + V326 
                 Q169 + M246 
                 A174 + A225 
                 Q125 + E190 
               
               
                 Q98 + Q169 
                 A186 + L250 
                 G142 + Q169 
                 Q169 + M248 
                 A174 + K242 
                 Q125 + A225 
               
               
                 Q98 + A186 
                 A186 + G255 
                 G142 + A186 
                 Q169 + L250 
                 A174 + S244 
                 Q125 + K242 
               
               
                 Q98 + E190 
                 A186 + Q256 
                 G142 + E190 
                 Q169 + G255 
                 A174 + N260 
                 Q125 + S244 
               
               
                 Q98 + A225 
                 A186 + N260 
                 G142 + A225 
                 Q169 + Q256 
                 A174 + K269 
                 Q125 + N260 
               
               
                 Q98 + K242 
                 A186 + A263 
                 G142 + K242 
                 Q169 + N260 
                 A174 + W284 
                 Q125 + K269 
               
               
                 Q98 + S244 
                 A186 + V264 
                 G142 + S244 
                 Q169 + A263 
                 A174 + Y295 
                 Q125 + W284 
               
               
                 Q98 + N260 
                 A186 + A265 
                 G142 + N260 
                 Q169 + V264 
                 A174 + V326 
                 Q125 + Y295 
               
               
                 Q98 + K269 
                 A186 + Y267 
                 G142 + K269 
                 Q169 + A265 
                 T193 + A225 
                 Q125 + V326 
               
               
                 Q98 + W284 
                 A186 + K269 
                 G142 + W284 
                 Q169 + Y267 
                 T193 + K242 
                 V215 + A225 
               
               
                 Q98 + Y295 
                 A186 + N270 
                 G142 + Y295 
                 Q169 + K269 
                 T193 + S244 
                 V215 + K242 
               
               
                 Q98 + V326 
                 A186 + G273 
                 G142 + V326 
                 Q169 + N270 
                 T193 + N260 
                 V215 + S244 
               
               
                 M105 + Q169 
                 A186 + S280 
                 G149 + Q169 
                 Q169 + G273 
                 T193 + K269 
                 V215 + N260 
               
               
                 M105 + A186 
                 A186 + W284 
                 G149 + A186 
                 Q169 + S280 
                 T193 + W284 
                 V215 + K269 
               
               
                 M105 + E190 
                 A186 + T285 
                 G149 + E190 
                 Q169 + W284 
                 T193 + Y295 
                 V215 + W284 
               
               
                 M105 + A225 
                 A186 + M286 
                 G149 + A225 
                 Q169 + T285 
                 T193 + V326 
                 V215 + Y295 
               
               
                 M105 + K242 
                 A186 + F289 
                 G149 + K242 
                 Q169 + M286 
                 N195 + A225 
                 V215 + V326 
               
               
                 M105 + S244 
                 A186 + V291 
                 G149 + S244 
                 Q169 + F289 
                 N195 + K242 
                 L217 + A225 
               
               
                 M105 + N260 
                 A186 + Y295 
                 G149 + N260 
                 Q169 + V291 
                 N195 + S244 
                 L217 + K242 
               
               
                 M105 + K269 
                 A186 + Q299 
                 G149 + K269 
                 Q169 + Y295 
                 N195 + N260 
                 L217 + S244 
               
               
                 M105 + W284 
                 A186 + S304 
                 G149 + W284 
                 Q169 + Q299 
                 N195 + K269 
                 L217 + N260 
               
               
                 M105 + Y295 
                 A186 + R320 
                 G149 + Y295 
                 Q169 + S304 
                 N195 + W284 
                 L217 + K269 
               
               
                 M105 + V326 
                 A186 + H321 
                 G149 + V326 
                 Q169 + R320 
                 N195 + Y295 
                 L217 + W284 
               
               
                 A225 + L228 
                 A186 + S323 
                 T165 + Q169 
                 Q169 + H321 
                 N195 + V326 
                 L217 + Y295 
               
               
                 A225 + L235 
                 A186 + H324 
                 T165 + A186 
                 Q169 + S323 
                 G196 + A225 
                 L217 + V326 
               
               
                 A225 + V238 
                 A186 + V326 
                 T165 + E190 
                 Q169 + H324 
                 G196 + K242 
                 L219 + A225 
               
               
                 A225 + K242 
                 A186 + F328 
                 T165 + A225 
                 Q169 + V326 
                 G196 + K242 
                 L219 + K242 
               
               
                 A225 + S244 
                 A186 + T334 
                 T165 + K242 
                 Q169 + F328 
                 G196 + S244 
                 L219 + S244 
               
               
                 A225 + M246 
                 A186 + D337 
                 T165 + S244 
                 Q169 + T334 
                 G196 + N260 
                 L219 + N260 
               
               
                 A225 + M248 
                 A186 + Q345 
                 T165 + N260 
                 Q169 + D337 
                 G196 + K269 
                 L219 + K269 
               
               
                 A225 + L250 
                 A186 + G346 
                 T165 + K269 
                 Q169 + Q345 
                 G196 + W284 
                 L219 + W284 
               
               
                 A225 + G255 
                 A186 + G348 
                 T165 + W284 
                 Q169 + G346 
                 G196 + Y295 
                 L219 + Y295 
               
               
                 A225 + Q256 
                 A186 + T355 
                 T165 + Y295 
                 Q169 + G348 
                 G196 + V326 
                 L219 + V326 
               
               
                 A225 + N260 
                 A186 + S376 
                 T165 + V326 
                 Q169 + T355 
                 A204 + A225 
                 M246 + N260 
               
               
                 A225 + A263 
                 A186 + D377 
                 L228 + K242 
                 Q169 + S376 
                 A204 + K242 
                 M246 + K269 
               
               
                 A225 + V264 
                 A186 + D379 
                 L228 + S244 
                 Q169 + D377 
                 A204 + S244 
                 M246 + W284 
               
               
                 A225 + A265 
                 A186 + Y382 
                 L228 + N260 
                 Q169 + D379 
                 A204 + N260 
                 M246 + Y295 
               
               
                 A225 + Y267 
                 A186 + S383 
                 L228 + K269 
                 Q169 + Y382 
                 A204 + K269 
                 M246 + V326 
               
               
                 A225 + K269 
                 A186 + Q385 
                 L228 + W284 
                 Q169 + S383 
                 A204 + W284 
                 M248 + N260 
               
               
                 A225 + N270 
                 A186 + K391 
                 L228 + Y295 
                 Q169 + Q385 
                 A204 + Y295 
                 M248 + K269 
               
               
                 A225 + G273 
                 A186 + K393 
                 L228 + V326 
                 Q169 + K391 
                 A204 + V326 
                 M248 + W284 
               
               
                 A225 + S280 
                 A186 + Q395 
                 L235 + K242 
                 Q169 + K393 
                 V206 + A225 
                 M248 + Y295 
               
               
                 A225 + W284 
                 E190 + T193 
                 L235 + S244 
                 Q169 + Q395 
                 A206 + K242 
                 M248 + V326 
               
               
                 A225 + T285 
                 E190 + N195 
                 L235 + N260 
                 Q256 + N260 
                 A206 + S244 
                 L250 + N260 
               
               
                 A225 + M286 
                 E190 + G196 
                 L235 + K269 
                 G256 + K269 
                 A206 + N260 
                 L250 + K269 
               
               
                 A225 + F289 
                 E190 + A204 
                 L235 + W284 
                 Q256 + W284 
                 A206 + K269 
                 L250 + W284 
               
               
                 A225 + V291 
                 E190 + V206 
                 L235 + Y295 
                 Q256 + Y295 
                 A206 + W284 
                 L250 + Y295 
               
               
                 A225 + Y295 
                 E190 + P211 
                 L235 + V326 
                 Q256 + V326 
                 A206 + Y295 
                 L250 + V326 
               
               
                 A225 + Q299 
                 E190 + I214 
                 V238 + K242 
                 N260 + A263 
                 A206 + V326 
                 G255 + N260 
               
               
                 A225 + S304 
                 E190 + V215 
                 V238 + S244 
                 N260 + V264 
                 P211 + A225 
                 G255 + K269 
               
               
                 A225 + R320 
                 E190 + L217 
                 V238 + N260 
                 N260 + A265 
                 P211 + K242 
                 G255 + W284 
               
               
                 A225 + H321 
                 E190 + L219 
                 V238 + K269 
                 N260 + Y267 
                 P211 + S244 
                 G255 + Y295 
               
               
                 A225 + S323 
                 E190 + A225 
                 V238 + W284 
                 N260 + K269 
                 P211 + N206 
                 G255 + V326 
               
               
                 A225 + H324 
                 E190 + L228 
                 V238 + Y295 
                 N260 + N270 
                 P211 + K269 
                 N270 + W284 
               
               
                 A225 + V326 
                 E190 + L235 
                 V238 + V326 
                 N260 + G273 
                 P211 + W284 
                 N270 + Y295 
               
               
                 A225 + F328 
                 E190 + V238 
                 K242 + S244 
                 N260 + S280 
                 P211 + Y295 
                 N270 + V326 
               
               
                 A225 + T334 
                 E190 + K242 
                 K242 + M246 
                 N260 + W284 
                 P211 + V326 
                 G273 + W284 
               
               
                 A225 + D337 
                 E190 + S244 
                 K242 + M248 
                 N260 + T285 
                 I214 + A225 
                 G273 + Y295 
               
               
                 A225 + Q345 
                 E190 + M246 
                 K242 + L250 
                 N260 + M286 
                 I214 + K242 
                 G273 + V326 
               
               
                 A225 + G346 
                 E190 + M248 
                 K242 + G255 
                 N260 + F289 
                 I214 + S244 
                 S280 + W284 
               
               
                 A225 + G348 
                 E190 + L250 
                 K242 + Q256 
                 N260 + V291 
                 I214 + N260 
                 S280 + Y295 
               
               
                 A225 + T355 
                 E190 + G255 
                 K242 + N260 
                 N260 + Y295 
                 I214 + K269 
                 S280 + V326 
               
               
                 A225 + S376 
                 E190 + Q256 
                 K242 + A263 
                 N260 + Q299 
                 I214 + W284 
                 W284 + T285 
               
               
                 A225 + D377 
                 E190 + N260 
                 K242 + V264 
                 N260 + S304 
                 I214 + Y295 
                 W284 + M286 
               
               
                 A225 + D379 
                 E190 + A263 
                 K242 + A265 
                 N260 + R320 
                 I214 + V326 
                 W284 + F289 
               
               
                 A225 + Y382 
                 E190 + V264 
                 K242 + Y267 
                 N260 + H321 
                 K269 + N270 
                 W284 + V291 
               
               
                 A225 + S383 
                 E190 + A265 
                 K242 + K269 
                 N260 + S323 
                 K269 + G273 
                 W284 + Y295 
               
               
                 A225 + Q385 
                 E190 + Y267 
                 K242 + N270 
                 N260 + H324 
                 K269 + S280 
                 W284 + Q299 
               
               
                 A225 + K391 
                 E190 + K269 
                 K242 + G273 
                 N260 + V326 
                 K269 + W284 
                 W284 + S304 
               
               
                 A225 + K393 
                 E190 + N270 
                 K242 + S280 
                 N260 + F328 
                 K269 + T285 
                 W284 + R320 
               
               
                 A225 + Q395 
                 E190 + G273 
                 K242 + W284 
                 N260 + T334 
                 K269 + M286 
                 W284 + H321 
               
               
                 A263 + K269 
                 E190 + S280 
                 K242 + T285 
                 N260 + D337 
                 K269 + F289 
                 W284 + S323 
               
               
                 A263 + W284 
                 E190 + W284 
                 K242 + M286 
                 N260 + Q345 
                 K269 + V291 
                 W284 + H324 
               
               
                 A263 + Y295 
                 E190 + T285 
                 K242 + F289 
                 N260 + G346 
                 K269 + Y295 
                 W284 + V326 
               
               
                 A263 + V326 
                 E190 + M286 
                 K242 + V291 
                 N260 + G348 
                 K269 + Q299 
                 W284 + F328 
               
               
                 V264 + K269 
                 E190 + F289 
                 K242 + Y295 
                 N260 + T355 
                 K269 + S304 
                 W284 + T334 
               
               
                 V264 + W284 
                 E190 + V291 
                 K242 + Q299 
                 N260 + S376 
                 K269 + R320 
                 W284 + D337 
               
               
                 V264 + Y295 
                 E190 + Y295 
                 K242 + S304 
                 N260 + D377 
                 K269 + H321 
                 W284 + Q345 
               
               
                 V264 + V326 
                 E190 + Q299 
                 K242 + R320 
                 N260 + D379 
                 K269 + S323 
                 W284 + G346 
               
               
                 A265 + K269 
                 E190 + S304 
                 K242 + H321 
                 N260 + Y382 
                 K269 + H324 
                 W284 + G348 
               
               
                 A265 + W284 
                 E190 + R320 
                 K242 + S323 
                 N260 + S383 
                 K269 + V326 
                 W284 + T355 
               
               
                 A265 + Y295 
                 E190 + H321 
                 K242 + H324 
                 N260 + Q385 
                 K269 + F328 
                 W284 + S376 
               
               
                 A265 + V326 
                 E190 + S323 
                 K242 + V326 
                 N260 + K391 
                 K269 + T334 
                 W284 + D377 
               
               
                 A265 + K269 
                 E190 + H324 
                 K242 + F328 
                 N260 + K393 
                 K269 + D337 
                 W284 + D379 
               
               
                 A265 + W284 
                 E190 + V326 
                 K242 + T334 
                 N260 + Q395 
                 K269 + Q345 
                 W284 + Y382 
               
               
                 A265 + Y295 
                 E190 + F328 
                 K242 + D337 
                 Y295 + S376 
                 K269 + G346 
                 W284 + S383 
               
               
                 A265 + V326 
                 E190 + T334 
                 K242 + Q345 
                 V326 + S376 
                 K269 + G348 
                 W284 + Q385 
               
               
                 Y295 + D377 
                 E190 + D337 
                 K242 + G346 
                 V326 + D377 
                 K269 + T355 
                 W284 + K391 
               
               
                 Y295 + D379 
                 E190 + Q345 
                 K242 + G348 
                 V326 + D379 
                 K269 + S376 
                 W284 + K393 
               
               
                 Y295 + Y382 
                 E190 + G346 
                 K242 + T355 
                 V326 + Y382 
                 K269 + D377 
                 W284 + Q395 
               
               
                 Y295 + S383 
                 E190 + G348 
                 K242 + S376 
                 V326 + S383 
                 K269 + D379 
                 T285 + Y295 
               
               
                 Y295 + Q385 
                 E190 + T355 
                 K242 + D377 
                 V326 + Q385 
                 K269 + Y382 
                 T285 + V326 
               
               
                 Y295 + K391 
                 E190 + S376 
                 K242 + D379 
                 V326 + K391 
                 K269 + S383 
                 M286 + Y295 
               
               
                 Y295 + K393 
                 E190 + D377 
                 K242 + Y382 
                 V326 + K393 
                 K269 + Q385 
                 M286 + V326 
               
               
                 Y295 + Q395 
                 E190 + D379 
                 K242 + S383 
                 V326 + Q395 
                 K269 + K391 
                 F289 + Y295 
               
               
                 Q299 + V326 
                 E190 + Y382 
                 K242 + Q385 
                 S244 + K393 
                 K269 + K393 
                 F289 + V326 
               
               
                 S304 + V326 
                 E190 + S383 
                 K242 + K391 
                 S244 + Q395 
                 K269 + Q395 
                 V291 + Y295 
               
               
                 R320 + V326 
                 E190 + Q385 
                 K242 + K393 
                 S244 + Y267 
                 S244 + R320 
                 V294 + V326 
               
               
                 H321 + V326 
                 E190 + K391 
                 K242 + Q395 
                 S244 + K269 
                 S244 + H321 
                 Y295 + Q299 
               
               
                 S323 + V326 
                 E190 + K393 
                 S244 + M246 
                 S244 + N270 
                 S244 + S323 
                 Y295 + S304 
               
               
                 H324 + V326 
                 E190 + Q395 
                 S244 + M248 
                 S244 + G273 
                 S244 + H324 
                 Y295 + R320 
               
               
                 V326 + F328 
                 S244 + F289 
                 S244 + L250 
                 S244 + S280 
                 S244 + V326 
                 Y295 + H321 
               
               
                 V326 + T334 
                 S244 + V291 
                 S244 + G255 
                 S244 + W284 
                 S244 + F328 
                 Y295 + S323 
               
               
                 V326 + D337 
                 S244 + Y295 
                 S244 + Q256 
                 S244 + T285 
                 S244 + T334 
                 Y295 + H324 
               
               
                 V326 + Q345 
                 S244 + Q299 
                 S244 + N260 
                 S244 + S376 
                 S244 + D337 
                 Y295 + V326 
               
               
                 V326 + G346 
                 S244 + S304 
                 S244 + A263 
                 S244 + D377 
                 S244 + Q345 
                 Y295 + F328 
               
               
                 V326 + G348 
                 S244 + K391 
                 S244 + V264 
                 S244 + D379 
                 S244 + G346 
                 Y295 + T334 
               
               
                 V326 + T355 
                 S244 + M286 
                 S244 + A265 
                 S244 + Y382 
                 S244 + G348 
                 Y295 + F328 
               
               
                 Y295 + G348 
                 Y295 + Q345 
                   
                 S244 + S383 
                 S244 + T355 
                 Y295 + T334 
               
               
                 Y295 + T355 
                 Y295 + G346 
                   
                 S244 + Q385 
                   
                 Y295 + D337 
               
               
                   
               
            
           
         
       
     
     In one particular embodiment, the modifications in one or more positions correspond to the following; H1*, T5K, G7K, G7A, Q11H, N16S, N16H, V17L, Q323, A37H, A37M, A37V, A373, A37Y, A37R, A37L, T40D, T40G, T40K, P45A, W48G, W48Y, W48F, G50A, T51K, T51E, T51G, T51A, T51S, T51G, T51D, N54S, V56T, A60V, K72R, K72H, K72S, K72Q, K72E, K72N, K72A, K72M, R87S, Q983, Q98A, M105F, M105I, M105V, M105L, G109A, G109S, F113W, F113S, F113N, F113Y, F113R, F113L, F113Q, R116Q, R116V, R116K, R116W, R116L, R116A, R116H, R116M, R116E, R116S, R116I, R116G, Q118N, Q118K, Q118G, Q118S, Q118F, Q118R, Q125P, Q125K, Q125A, Q125T, N125D, G133S, F133Q, T134E, W140Y, G142T, G149Q, T165S, T165G, T165V, W167I, W167G, W167F, W167R, W167S, W167H, W167L, W167M, W167Y, Q169E, R171H, Q172G, Q172R, Q172N, Q172D, Q172Y, Q172M, Q172S, Q172T, Q172K, Q172H, Q172E, L173V, L173G, L173H, L173A, L173I, L173P, L173T, L173F, L173M, A174S, A174D, A174P, A174M, A174T, A174H, A174K, A174G, A174Q, A174N, A174V, A174L, G184T, A186D, A186N, A186E, A186Q, A186H, E190P, T193K, N195F, G196R, A204T, A204V, A204S, A204G, V206L, V206S, V206Y, P211D, I214G, I214H, I214S, 214T, I214L, I214E, I214W, V215T, L217T, L217Q, L219V, L219H, A225V, L228I, L235V, L235A, V238A, V238T, V238G, K242Q, S244Q, M246L, M246A, M246I, M246F, M246V, M246S, M248T, L250I, L250V, L250T, L250A, L250F, L250M, G255N, G255A, G255A, Q256A, N260G, A263G, V264I, V264T, A265G, Y267I, Y267M, Y267H, Y267L, K269Q, N270G, N270I, G273R, S280W, S280L, S280T, S280A, S280K, S280Q, W284H, T285L, T285Q, M286F, M286L, A288L, A288V, F289I, F289L, V291G, V291A, V291T, Y295N, Y295F, Q299V, S304N, S304R, R320A, R320V, H321Y, S323N, H324L, V326L, F328L, F328M, F328V, F328I, T334S, D337H, Q345D, G346T, G346D, G346P, G348S, G348P, T355L, T355F, S376H, S376I, S376V, D377H, D377S, D377A, D377Q, D379S, D379G, D379R, D379A, Y382M, Y382I, Y382L, Y382F, S383G, S383A, Q385L, K391A, K391Y, K391V, K391M, K391E, K391D, K391R, K391H, K391W, K391I, K391Q, K391L, K393Y, K393R, K393Q, K393S, and Q395P, wherein said positions correspond to positions of SEQ ID NOs: 7 or 10. 
     The inventors of the present invention have identified that these specific alterations within the A and B domain (as defined by SEQ ID NO: 10) of the amino acid sequence as set forth in SEQ ID NOs: 7 and 10, are particularly relevant for improving the performance of a variant alpha-amylase having at least 65% sequence identity to the parent polypeptide. 
     In one embodiment, the variant comprises an alteration in two, three, four, five, six, seven, eight, nine, ten, eleven, twelf, thirteen, fourteen, fifteen, sixteen, or seventeen positions corresponding to the following positions: H1*, T5K, G7K, G7A, Q11H, N16S, N16H, V17L, Q32S, A37H, A37M, A37V, A37S, A37Y, A37R, A37L, T40D, T40G, T40K, P45A, W48G, W48Y, W48F, G50A, T51K, T51E, T51G, T51A, T51S, T51G, T51D, N54S, V56T, A60V, K72R, K72H, K72S, K72Q, K72E, K72N, K72A, K72M, R87S, Q98S, Q98A, M105F, M105I, M105V, M105L, G109A, G109S, F113W, F113S, F113N, F113Y, F113R, F113L, F113Q, R116Q, R116V, R116K, R116W, R116L, R116A, R116H, R116M, R116E, R116S, S116I, R116G, Q118N, Q118K, Q118G, Q118S, Q118F, Q118R, Q125P, Q125K, Q125A, Q125T, N125D, G133S, F133Q, T134E, W140Y, G142T, G149Q, T165S, T165G, I165V, W167I, W167G, W167F, W167R, W167S, W167H, W167L, W167M, W167Y, Q169E, R171H, Q172G, Q172R, Q172N, Q172D, Q172Y, Q172M, Q172S, Q172I, Q172K, Q172H, Q172E, L173V, L173G, L173H, L173A, L173I, L173P, L173T, L173F, L173M, A174S, A174D, A174P, A174M, A174T, A174H, A174K, A174G, A174Q, A174N, A174V, A174L, G184I, A186D, A186N, A186E, A186Q, A186H, E190P, T193K, N195F, G196R, A204T, A204V, A204S, A204G, V206L, V206S, V206Y, P211D, I214G, I214H, I214S, I214T, I214L, I214E, I214W, V215T, L217T, L217Q, L219V, L219H, A225V, L228I, L235V, L235A, V238A, V238T, V238G, K242Q, S244Q, M246L, M246A, M246I, M246F, M246V, M246S, M248T, L250I, L250V, L250T, L250A, L250F, L250M, G255N, G255A, G255S, Q256A, N260G, A263G, V264I, V264T, A265G, Y267I, Y267M, Y267H, Y267L, K269Q, N270G, N270T, G273R, S280W, S280L, S280T, S280A, S280K, S280Q, W284H, T285L, T285Q, M286F, M286L, A288L, A288V, F289I, F289L, V291G, V291A, V291T, Y295N, Y295F, Q299V, S304N, S304R, R320A, R320V, H321Y, S323N, H324L, V326L, F328L, F328M, F328V, F328I, T334S, D337H, Q345D, G346T, G346D, G346P, G348S, G348P, T355L, T355F, S376H, S376T, S376V, D377H, D377S, D377A, D377Q, D379S, D379G, D379R, D379A, Y382M, Y382I, Y382L, Y382F, S383G, S383A, Q385L, K391A, K391Y, K391V, K391M, K391E, K391D, K391R, K391H, K391W, K391I, K391Q, K391L, K393Y, K393R, K393Q, K393S, and Q395P, wherein said positions correspond to positions of SEQ ID NOs: 7 or 10. 
     The C domain of a variant according to the present invention may also comprise alterations. Thus, in one embodiment, the variant comprises an alteration in one or more amino acid positions corresponding to the amino acid positions of the amino acid sequence set forth in SEQ ID NOs: 11 or 12, or an amino acid sequence having at least 65% sequence identity to an amino acid sequence as set forth in SEQ ID NOs: 11 or 12. 
     In one particular embodiment, the C domain comprises an alteration in one or more of the following positions corresponding to positions; A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ1D NO: 13 or I405, A421, A422, A428, R439 G448, W467, D476, and G477 of SEQ ID NO: 14. 
     In one embodiment, the variant comprises an alteration in two, three, four, five, six, seven, or eight positions corresponding to the following positions; A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13 or I405, A421, A422, A428, R439 G448, W467, D476, and G477 of SEQ ID NO: 14. 
     The inventors of the present invention have identified that specific amino acid substitutions within the C domain of parent polypeptide have an improved effect on performance of the variants. Thus, in a particular embodiment, the alteration in one or more positions correspond to the following T400P, H402R, A420Q, A420S, A420K, A420L, G423H, T444Q, T444S, T444D, T444Y, T444H, T444V, T444R, T444A, A445Q, Q449T, T459N, P473R, P473A, P473G, P473T, P473K, C474V, G476K, G477A, G477Q, K484A, K484G, K484P, K484E, K484Q, and K484S, wherein said positions correspond to positions of SEQ ID NO: 13; or I405L. A421H, A422P, A428T, G448D, D476K, D476G, D476N, D476Y, G477S, G477A, G477T, and G477Q, wherein said positions correspond to positions of SEQ ID NO: 14. 
     In a particular embodiment, the variant comprises a modification in the following positions; 
                                                    H1 + T5   T40 + A174   R87 + N270   W140 + K393   V206 + V215   Y267 + C474       H1 + G7   T40 + G184   R87 + G273   W140 + Q395   V206 + L217   Y267 + G476       H1 + Q11   T40 + T193   R87 + S280   W140 + A420   V206 + L219   Y267 + G477       H1 + N16   T40 + N195   R87 + T285   W140 + G423   V206 + L235   Y267 + K484       H1 + V17   T40 + A204   R87 + M286   W140 + T444   V206 + V238   N270 + G273       H1 + Q32   T40 + V206   R87 + F289   W140 + A445   V206 + M246   N270 + S280       H1 + A37   T40 + P211   R87 + V291   W140 + Q449   V206 + M248   N270 + T285       H1 + T40   T40 + I214   R87 + Q299   W140 + T459   V206 + L250   N270 + M286       H1 + P45   T40 + V215   R87 + R320   W140 + P473   V206 + G255   N270 + F289       H1 + W48   T40 + L217   R87 + H321   W140 + C474   V206 + Q256   N270 + V291       H1 + G50   T40 + L219   R87 + S323   W140 + G476   V206 + A263   N270 + Q299       H1 + T51   T40 + L235   R87 + H324   W140 + G477   V206 + V264   N270 + R320       H1 + N54   T40 + V238   R87 + F328   W140 + K484   V206 + Y267   N270 + H321       H1 + V56   T40 + M246   R87 + T334   G142 + G149   V206 + N270   N270 + S323       H1 + K72   T40 + M248   R87 + D337   G142 + T165   V206 + G273   N270 + H324       H1 + R87   T40 + L250   R87 + Q345   G142 + W167   V206 + S280   N270 + F328       H1 + Q98   T40 + G255   R87 + G346   G142 + R171   V206 + T285   N270 + T334       H1 + M105   T40 + Q256   R87 + G348   G142 + Q172   V206 + M286   N270 + D337       H1 + G109   T40 + A263   R87 + T355   G142 + L173   V206 + F289   N270 + Q345       H1 + F113   T40 + V264   R87 + S376   G142 + A174   V206 + V291   N270 + G346       H1 + R116   T40 + Y267   R87 + D377   G142 + G184   V206 + Q299   N270 + G348       H1 + Q118   T40 + N270   R87 + D379   G142 + T193   V206 + R320   N270 + T355       H1 + Q125   T40 + G273   R87 + Y382   G142 + N195   V206 + H321   N270 + S376       H1 + G133   T40 + S280   R87 + S383   G142 + A204   V206 + S323   N270 + D377       H1 + T134   T40 + T285   R87 + Q385   G142 + V206   V206 + H324   N270 + D379       H1 + W140   T40 + M286   R87 + K391   G142 + P211   V206 + F328   N270 + Y382       H1 + G142   T40 + F289   R87 + K393   G142 + I214   V206 + T334   N270 + S383       H1 + G149   T40 + V291   R87 + Q395   G142 + V215   V206 + D337   N270 + Q385       H1 + T165   T40 + Q299   R87 + A420   G142 + L217   V206 + Q345   N270 + K391       H1 + W167   T40 + R320   R87 + G423   G142 + L219   V206 + G346   N270 + K393       H1 + R171   T40 + H321   R87 + T444   G142 + L235   V206 + G348   N270 + Q395       H1 + Q172   T40 + S323   R87 + A445   G142 + V238   V206 + T355   N270 + A420       H1 + L173   T40 + H324   R87 + Q449   G142 + M246   V206 + S376   N270 + G423       H1 + A174   T40 + F328   R87 + T459   G142 + M248   V206 + D377   N270 + T444       H1 + G184   T40 + T334   R87 + P473   G142 + L250   V206 + D379   N270 + A445       H1 + T193   T40 + D337   R87 + C474   G142 + G255   V206 + Y382   N270 + Q449       H1 + N195   T40 + Q345   R87 + G476   G142 + Q256   V206 + S383   N270 + T459       H1 + A204   T40 + G346   R87 + G477   G142 + A263   V206 + Q385   N270 + P473       H1 + V206   T40 + G348   R87 + K484   G142 + V264   V206 + K391   N270 + C474       H1 + P211   T40 + T355   Q98 + M105   G142 + Y267   V206 + K393   N270 + G476       H1 + I214   T40 + S376   Q98 + G109   G142 + N270   V206 + Q395   N270 + G477       H1 + V215   T40 + D377   Q98 + F113   G142 + G273   V206 + A420   N270 + K484       H1 + L217   T40 + D379   Q98 + R116   G142 + S280   V206 + G423   G273 + S280       H1 + L219   T40 + Y382   Q98 + Q118   G142 + T285   V206 + T444   G273 + T285       H1 + L235   T40 + S383   Q98 + Q125   G142 + M286   V206 + A445   G273 + M286       H1 + V238   T40 + Q385   Q98 + G133   G142 + F289   V206 + Q449   G273 + F289       H1 + M246   T40 + K391   Q98 + T134   G142 + V291   V206 + T459   G273 + V291       H1 + M248   T40 + K393   Q98 + W140   G142 + Q299   V206 + P473   G273 + Q299       H1 + L250   T40 + Q395   Q98 + G142   G142 + R320   V206 + C474   G273 + R320       H1 + G255   T40 + A420   Q98 + G149   G142 + H321   V206 + G476   G273 + H321       H1 + Q256   T40 + G423   Q98 + T165   G142 + S323   V206 + G477   G273 + S323       H1 + A263   T40 + T444   Q98 + W167   G142 + H324   V206 + K484   G273 + H324       H1 + V264   T40 + A445   Q98 + R171   G142 + F328   P211 + I214   G273 + F328       H1 + Y267   T40 + Q449   Q98 + Q172   G142 + T334   P211 + V215   G273 + T334       H1 + N270   T40 + T459   Q98 + L173   G142 + D337   P211 + L217   G273 + D337       H1 + G273   T40 + P473   Q98 + A174   G142 + Q345   P211 + L219   G273 + Q345       H1 + S280   T40 + C474   Q98 + G184   G142 + G346   P211 + L235   G273 + G346       H1 + T285   T40 + G476   Q98 + T193   G142 + G348   P211 + V238   G273 + G348       H1 + M286   T40 + G477   Q98 + N195   G142 + T355   P211 + M246   G273 + T355       H1 + F289   T40 + K484   Q98 + A204   G142 + S376   P211 + M248   G273 + S376       H1 + V291   P45 + W48   Q98 + V206   G142 + D377   P211 + L250   G273 + D377       H1 + Q299   P45 + G50   Q98 + P211   G142 + D379   P211 + G255   G273 + D379       H1 + R320   P45 + T51   Q98 + I214   G142 + Y382   P211 + Q256   G273 + Y382       H1 + H321   P45 + N54   Q98 + V215   G142 + S383   P211 + A263   G273 + S383       H1 + S323   P45 + V56   Q98 + L217   G142 + Q385   P211 + V264   G273 + Q385       H1 + H324   P45 + K72   Q98 + L219   G142 + K391   P211 + Y267   G273 + K391       H1 + F328   P45 + R87   Q98 + L235   G142 + K393   P211 + N270   G273 + K393       H1 + T334   P45 + Q98   Q98 + V238   G142 + Q395   P211 + G273   G273 + Q395       H1 + D337   P45 + M105   Q98 + M246   G142 + A420   P211 + S280   G273 + A420       H1 + Q345   P45 + G109   Q98 + M248   G142 + G423   P211 + T285   G273 + G423       H1 + G346   P45 + F113   Q98 + L250   G142 + T444   P211 + M286   G273 + T444       H1 + G348   P45 + R116   Q98 + G255   G142 + A445   P211 + F289   G273 + A445       H1 + T355   P45 + Q118   Q98 + Q256   G142 + Q449   P211 + V291   G273 + Q449       H1 + S376   P45 + Q125   Q98 + A263   G142 + T459   P211 + Q299   G273 + T459       H1 + D377   P45 + G133   Q98 + V264   G142 + P473   P211 + R320   G273 + P473       H1 + D379   P45 + T134   Q98 + Y267   G142 + C474   P211 + H321   G273 + C474       H1 + Y382   P45 + W140   Q98 + N270   G142 + G476   P211 + S323   G273 + G476       H1 + S383   P45 + G142   Q98 + G273   G142 + G477   P211 + H324   G273 + G477       H1 + Q385   P45 + G149   Q98 + S280   G142 + K484   P211 + F328   G273 + K484       H1 + K391   P45 + T165   Q98 + T285   G149 + T165   P211 + T334   S280 + T285       H1 + K393   P45 + W167   Q98 + M286   G149 + W167   P211 + D337   S280 + M286       H1 + Q395   P45 + R171   Q98 + F289   G149 + R171   P211 + Q345   S280 + F289       H1 + A420   P45 + Q172   Q98 + V291   G149 + Q172   P211 + G346   S280 + V291       H1 + G423   P45 + L173   Q98 + Q299   G149 + L173   P211 + G348   S280 + Q299       H1 + T444   P45 + A174   Q98 + R320   G149 + A174   P211 + T355   S280 + R320       H1 + A445   P45 + G184   Q98 + H321   G149 + G184   P211 + S376   S280 + H321       H1 + Q449   P45 + T193   Q98 + S323   G149 + T193   P211 + D377   S280 + S323       H1 + T459   P45 + N195   Q98 + H324   G149 + N195   P211 + D379   S280 + H324       H1 + P473   P45 + A204   Q98 + F328   G149 + A204   P211 + Y382   S280 + F328       H1 + C474   P45 + V206   Q98 + T334   G149 + V206   P211 + S383   S280 + T334       H1 + G476   P45 + P211   Q98 + D337   G149 + P211   P211 + Q385   S280 + D337       H1 + G477   P45 + I214   Q98 + Q345   G149 + I214   P211 + K391   S280 + Q345       H1 + K484   P45 + V215   Q98 + G346   G149 + V215   P211 + K393   S280 + G346       T5 + G7   P45 + L217   Q98 + G348   G149 + L217   P211 + Q395   S280 + G348       T5 + Q11   P45 + L219   Q98 + T355   G149 + L219   P211 + A420   S280 + T355       T5 + N16   P45 + L235   Q98 + S376   G149 + L235   P211 + G423   S280 + S376       T5 + V17   P45 + V238   Q98 + D377   G149 + V238   P211 + T444   S280 + D377       T5 + Q32   P45 + M246   Q98 + D379   G149 + M246   P211 + A445   S280 + D379       T5 + A37   P45 + M248   Q98 + Y382   G149 + M248   P211 + Q449   S280 + Y382       T5 + T40   P45 + L250   Q98 + S383   G149 + L250   P211 + T459   S280 + S383       T5 + P45   P45 + G255   Q98 + Q385   G149 + G255   P211 + P473   S280 + Q385       T5 + W48   P45 + Q256   Q98 + K391   G149 + Q256   P211 + C474   S280 + K391       T5 + G50   P45 + A263   Q98 + K393   G149 + A263   P211 + G476   S280 + K393       T5 + T51   P45 + V264   Q98 + Q395   G149 + V264   P211 + G477   S280 + Q395       T5 + N54   P45 + Y267   Q98 + A420   G149 + Y267   P211 + K484   S280 + A420       T5 + V56   P45 + N270   Q98 + G423   G149 + N270   I214 + V215   S280 + G423       T5 + K72   P45 + G273   Q98 + T444   G149 + G273   I214 + L217   S280 + T444       T5 + R87   P45 + S280   Q98 + A445   G149 + S280   I214 + L219   S280 + A445       T5 + Q98   P45 + T285   Q98 + Q449   G149 + T285   I214 + L235   S280 + Q449       T5 + M105   P45 + M286   Q98 + T459   G149 + M286   I214 + V238   S280 + T459       T5 + G109   P45 + F289   Q98 + P473   G149 + F289   I214 + M246   S280 + P473       T5 + F113   P45 + V291   Q98 + C474   G149 + V291   I214 + M248   S280 + C474       T5 + R116   P45 + Q299   Q98 + G476   G149 + Q299   I214 + L250   S280 + G476       T5 + Q118   P45 + R320   Q98 + G477   G149 + R320   I214 + G255   S280 + G477       T5 + Q125   P45 + H321   Q98 + K484   G149 + H321   I214 + Q256   S280 + K484       T5 + G133   P45 + S323   M105 + G109   G149 + S323   I214 + A263   T285 + M286       T5 + T134   P45 + H324   M105 + F113   G149 + H324   I214 + V264   T285 + F289       T5 + W140   P45 + F328   M105 + R116   G149 + F328   I214 + Y267   T285 + V291       T5 + G142   P45 + T334   M105 + Q118   G149 + T334   I214 + N270   T285 + Q299       T5 + G149   P45 + D337   M105 + Q125   G149 + D337   I214 + G273   T285 + R320       T5 + T165   P45 + Q345   M105 + G133   G149 + Q345   I214 + S280   T285 + H321       T5 + W167   P45 + G346   M105 + T134   G149 + G346   I214 + T285   T285 + S323       T5 + R171   P45 + G348   M105 + W140   G149 + G348   I214 + M286   T285 + H324       T5 + Q172   P45 + T355   M105 + G142   G149 + T355   I214 + F289   T285 + F328       T5 + L173   P45 + S376   M105 + G149   G149 + S376   I214 + V291   T285 + T334       T5 + A174   P45 + D377   M105 + T165   G149 + D377   I214 + Q299   T285 + D337       T5 + G184   P45 + D379   M105 + W167   G149 + D379   I214 + R320   T285 + Q345       T5 + T193   P45 + Y382   M105 + R171   G149 + Y382   I214 + H321   T285 + G346       T5 + N195   P45 + S383   M105 + Q172   G149 + S383   I214 + S323   T285 + G348       T5 + A204   P45 + Q385   M105 + L173   G149 + Q385   I214 + H324   T285 + T355       T5 + V206   P45 + K391   M105 + A174   G149 + K391   I214 + F328   T285 + S376       T5 + P211   P45 + K393   M105 + G184   G149 + K393   I214 + T334   T285 + D377       T5 + I214   P45 + Q395   M105 + T193   G149 + Q395   I214 + D337   T285 + D379       T5 + V215   P45 + A420   M105 + N195   G149 + A420   I214 + Q345   T285 + Y382       T5 + L217   P45 + G423   M105 + A204   G149 + G423   I214 + G346   T285 + S383       T5 + L219   P45 + T444   M105 + V206   G149 + T444   I214 + G348   T285 + Q385       T5 + L235   P45 + A445   M105 + P211   G149 + A445   I214 + T355   T285 + K391       T5 + V238   P45 + Q449   M105 + I214   G149 + Q449   I214 + S376   T285 + K393       T5 + M246   P45 + T459   M105 + V215   G149 + T459   I214 + D377   T285 + Q395       T5 + M248   P45 + P473   M105 + L217   G149 + P473   I214 + D379   T285 + A420       T5 + L250   P45 + C474   M105 + L219   G149 + C474   I214 + Y382   T285 + G423       T5 + G255   P45 + G476   M105 + L235   G149 + G476   I214 + S383   T285 + T444       T5 + Q256   P45 + G477   M105 + V238   G149 + G477   I214 + Q385   T285 + A445       T5 + A263   P45 + K484   M105 + M246   G149 + K484   I214 + K391   T285 + Q449       T5 + V264   W48 + G50   M105 + M248   T165 + W167   I214 + K393   T285 + T459       T5 + Y267   W48 + T51   M105 + L250   T165 + R171   I214 + Q395   T285 + P473       T5 + N270   W48 + N54   M105 + G255   T165 + Q172   I214 + A420   T285 + C474       T5 + G273   W48 + V56   M105 + Q256   T165 + L173   I214 + G423   T285 + G476       T5 + S280   W48 + K72   M105 + A263   T165 + A174   I214 + T444   T285 + G477       T5 + T285   W48 + R87   M105 + V264   T165 + G184   I214 + A445   T285 + K484       T5 + M286   W48 + Q98   M105 + Y267   T165 + T193   I214 + Q449   M286 + F289       T5 + F289   W48 + M105   M105 + N270   T165 + N195   I214 + T459   M286 + V291       T5 + V291   W48 + G109   M105 + G273   T165 + A204   I214 + P473   M286 + Q299       T5 + Q299   W48 + F113   M105 + S280   T165 + V206   I214 + C474   M286 + R320       T5 + R320   W48 + R116   M105 + T285   T165 + P211   I214 + G476   M286 + H321       T5 + H321   W48 + Q118   M105 + M286   T165 + I214   I214 + G477   M286 + S323       T5 + S323   W48 + Q125   M105 + F289   T165 + V215   I214 + K484   M286 + H324       T5 + H324   W48 + G133   M105 + V291   T165 + L217   V215 + L217   M286 + F328       T5 + F328   W48 + T134   M105 + Q299   T165 + L219   V215 + L219   M286 + T334       T5 + T334   W48 + W140   M105 + R320   T165 + L235   V215 + L235   M286 + D337       T5 + D337   W48 + G142   M105 + H321   T165 + V238   V215 + V238   M286 + Q345       T5 + Q345   W48 + G149   M105 + S323   T165 + M246   V215 + M246   M286 + G346       T5 + G346   W48 + T165   M105 + H324   T165 + M248   V215 + M248   M286 + G348       T5 + G348   W48 + W167   M105 + F328   T165 + L250   V215 + L250   M286 + T355       T5 + T355   W48 + R171   M105 + T334   T165 + G255   V215 + G255   M286 + S376       T5 + S376   W48 + Q172   M105 + D337   T165 + Q256   V215 + Q256   M286 + D377       T5 + D377   W48 + L173   M105 + Q345   T165 + A263   V215 + A263   M286 + D379       T5 + D379   W48 + A174   M105 + G346   T165 + V264   V215 + V264   M286 + Y382       T5 + Y382   W48 + G184   M105 + G348   T165 + Y267   V215 + Y267   M286 + S383       T5 + S383   W48 + T193   M105 + T355   T165 + N270   V215 + N270   M286 + Q385       T5 + Q385   W48 + N195   M105 + S376   T165 + G273   V215 + G273   M286 + K391       T5 + K391   W48 + A204   M105 + D377   T165 + S280   V215 + S280   M286 + K393       T5 + K393   W48 + V206   M105 + D379   T165 + T285   V215 + T285   M286 + Q395       T5 + Q395   W48 + P211   M105 + Y382   T165 + M286   V215 + M286   M286 + A420       T5 + A420   W48 + I214   M105 + S383   T165 + F289   V215 + F289   M286 + G423       T5 + G423   W48 + V215   M105 + Q385   T165 + V291   V215 + V291   M286 + T444       T5 + T444   W48 + L217   M105 + K391   T165 + Q299   V215 + Q299   M286 + A445       T5 + A445   W48 + L219   M105 + K393   T165 + R320   V215 + R320   M286 + Q449       T5 + Q449   W48 + L235   M105 + Q395   T165 + H321   V215 + H321   M286 + T459       T5 + T459   W48 + V238   M105 + A420   T165 + S323   V215 + S323   M286 + P473       T5 + P473   W48 + M246   M105 + G423   T165 + H324   V215 + H324   M286 + C474       T5 + C474   W48 + M248   M105 + T444   T165 + F328   V215 + F328   M286 + G476       T5 + G476   W48 + L250   M105 + A445   T165 + T334   V215 + T334   M286 + G477       T5 + G477   W48 + G255   M105 + Q449   T165 + D337   V215 + D337   M286 + K484       T5 + K484   W48 + Q256   M105 + T459   T165 + Q345   V215 + Q345   F289 + V291       G7 + Q11   W48 + A263   M105 + P473   T165 + G346   V215 + G346   F289 + Q299       G7 + N16   W48 + V264   M105 + C474   T165 + G348   V215 + G348   F289 + R320       G7 + V17   W48 + Y267   M105 + G476   T165 + T355   V215 + T355   F289 + H321       G7 + Q32   W48 + N270   M105 + G477   T165 + S376   V215 + S376   F289 + S323       G7 + A37   W48 + G273   M105 + K484   T165 + D377   V215 + D377   F289 + H324       G7 + T40   W48 + S280   G109 + F113   T165 + D379   V215 + D379   F289 + F328       G7 + P45   W48 + T285   G109 + R116   T165 + Y382   V215 + Y382   F289 + T334       G7 + W48   W48 + M286   G109 + Q118   T165 + S383   V215 + S383   F289 + D337       G7 + G50   W48 + F289   G109 + Q125   T165 + Q385   V215 + Q385   F289 + Q345       G7 + T51   W48 + V291   G109 + G133   T165 + K391   V215 + K391   F289 + G346       G7 + N54   W48 + Q299   G109 + T134   T165 + K393   V215 + K393   F289 + G348       G7 + V56   W48 + R320   G109 + W140   T165 + Q395   V215 + Q395   F289 + T355       G7 + K72   W48 + H321   G109 + G142   T165 + A420   V215 + A420   F289 + S376       G7 + R87   W48 + S323   G109 + G149   T165 + G423   V215 + G423   F289 + D377       G7 + Q98   W48 + H324   G109 + T165   T165 + T444   V215 + T444   F289 + D379       G7 + M105   W48 + F328   G109 + W167   T165 + A445   V215 + A445   F289 + Y382       G7 + G109   W48 + T334   G109 + R171   T165 + Q449   V215 + Q449   F289 + S383       G7 + F113   W48 + D337   G109 + Q172   T165 + T459   V215 + T459   F289 + Q385       G7 + R116   W48 + Q345   G109 + L173   T165 + P473   V215 + P473   F289 + K391       G7 + Q118   W48 + G346   G109 + A174   T165 + C474   V215 + C474   F289 + K393       G7 + Q125   W48 + G348   G109 + G184   T165 + G476   V215 + G476   F289 + Q395       G7 + G133   W48 + T355   G109 + T193   T165 + G477   V215 + G477   F289 + A420       G7 + T134   W48 + S376   G109 + N195   T165 + K484   V215 + K484   F289 + G423       G7 + W140   W48 + D377   G109 + A204   W167 + R171   L217 + L219   F289 + T444       G7 + G142   W48 + D379   G109 + V206   W167 + Q172   L217 + L235   F289 + A445       G7 + G149   W48 + Y382   G109 + P211   W167 + L173   L217 + V238   F289 + Q449       G7 + T165   W48 + S383   G109 + I214   W167 + A174   L217 + M246   F289 + T459       G7 + W167   W48 + Q385   G109 + V215   W167 + G184   L217 + M248   F289 + P473       G7 + R171   W48 + K391   G109 + L217   W167 + T193   L217 + L250   F289 + C474       G7 + Q172   W48 + K393   G109 + L219   W167 + N195   L217 + G255   F289 + G476       G7 + L173   W48 + Q395   G109 + L235   W167 + A204   L217 + Q256   F289 + G477       G7 + A174   W48 + A420   G109 + V238   W167 + V206   L217 + A263   F289 + K484       G7 + G184   W48 + G423   G109 + M246   W167 + P211   L217 + V264   V291 + Q299       G7 + T193   W48 + T444   G109 + M248   W167 + I214   L217 + Y267   V291 + R320       G7 + N195   W48 + A445   G109 + L250   W167 + V215   L217 + N270   V291 + H321       G7 + A204   W48 + Q449   G109 + G255   W167 + L217   L217 + G273   V291 + S323       G7 + V206   W48 + T459   G109 + Q256   W167 + L219   L217 + S280   V291 + H324       G7 + P211   W48 + P473   G109 + A263   W167 + L235   L217 + T285   V291 + F328       G7 + I214   W48 + C474   G109 + V264   W167 + V238   L217 + M286   V291 + T334       G7 + V215   W48 + G476   G109 + Y267   W167 + M246   L217 + F289   V291 + D337       G7 + L217   W48 + G477   G109 + N270   W167 + M248   L217 + V291   V291 + Q345       G7 + L219   W48 + K484   G109 + G273   W167 + L250   L217 + Q299   V291 + G346       G7 + L235   G50 + T51   G109 + S280   W167 + G255   L217 + R320   V291 + G348       G7 + V238   G50 + N54   G109 + T285   W167 + Q256   L217 + H321   V291 + T355       G7 + M246   G50 + V56   G109 + M286   W167 + A263   L217 + S323   V291 + S376       G7 + M248   G50 + K72   G109 + F289   W167 + V264   L217 + H324   V291 + D377       G7 + L250   G50 + R87   G109 + V291   W167 + Y267   L217 + F328   V291 + D379       G7 + G255   G50 + Q98   G109 + Q299   W167 + N270   L217 + T334   V291 + Y382       G7 + Q256   G50 + M105   G109 + R320   W167 + G273   L217 + D337   V291 + S383       G7 + A263   G50 + G109   G109 + H321   W167 + S280   L217 + Q345   V291 + Q385       G7 + V264   G50 + F113   G109 + S323   W167 + T285   L217 + G346   V291 + K391       G7 + Y267   G50 + R116   G109 + H324   W167 + M286   L217 + G348   V291 + K393       G7 + N270   G50 + Q118   G109 + F328   W167 + F289   L217 + T355   V291 + Q395       G7 + G273   G50 + Q125   G109 + T334   W167 + V291   L217 + S376   V291 + A420       G7 + S280   G50 + G133   G109 + D337   W167 + Q299   L217 + D377   V291 + G423       G7 + T285   G50 + T134   G109 + Q345   W167 + R320   L217 + D379   V291 + T444       G7 + M286   G50 + W140   G109 + G346   W167 + H321   L217 + Y382   V291 + A445       G7 + F289   G50 + G142   G109 + G348   W167 + S323   L217 + S383   V291 + Q449       G7 + V291   G50 + G149   G109 + T355   W167 + H324   L217 + Q385   V291 + T459       G7 + Q299   G50 + T165   G109 + S376   W167 + F328   L217 + K391   V291 + P473       G7 + R320   G50 + W167   G109 + D377   W167 + T334   L217 + K393   V291 + C474       G7 + H321   G50 + R171   G109 + D379   W167 + D337   L217 + Q395   V291 + G476       G7 + S323   G50 + Q172   G109 + Y382   W167 + Q345   L217 + A420   V291 + G477       G7 + H324   G50 + L173   G109 + S383   W167 + G346   L217 + G423   V291 + K484       G7 + F328   G50 + A174   G109 + Q385   W167 + G348   L217 + T444   Q299 + R320       G7 + T334   G50 + G184   G109 + K391   W167 + T355   L217 + A445   Q299 + H321       G7 + D337   G50 + T193   G109 + K393   W167 + S376   L217 + Q449   Q299 + S323       G7 + Q345   G50 + N195   G109 + Q395   W167 + D377   L217 + T459   Q299 + H324       G7 + G346   G50 + A204   G109 + A420   W167 + D379   L217 + P473   Q299 + F328       G7 + G348   G50 + V206   G109 + G423   W167 + Y382   L217 + C474   Q299 + T334       G7 + T355   G50 + P211   G109 + T444   W167 + S383   L217 + G476   Q299 + D337       G7 + S376   G50 + I214   G109 + A445   W167 + Q385   L217 + G477   Q299 + Q345       G7 + D377   G50 + V215   G109 + Q449   W167 + K391   L217 + K484   Q299 + G346       G7 + D379   G50 + L217   G109 + T459   W167 + K393   L219 + L235   Q299 + G348       G7 + Y382   G50 + L219   G109 + P473   W167 + Q395   L219 + V238   Q299 + T355       G7 + S383   G50 + L235   G109 + C474   W167 + A420   L219 + M246   Q299 + S376       G7 + Q385   G50 + V238   G109 + G476   W167 + G423   L219 + M248   Q299 + D377       G7 + K391   G50 + M246   G109 + G477   W167 + T444   L219 + L250   Q299 + D379       G7 + K393   G50 + M248   G109 + K484   W167 + A445   L219 + G255   Q299 + Y382       G7 + Q395   G50 + L250   F113 + R116   W167 + Q449   L219 + Q256   Q299 + S383       G7 + A420   G50 + G255   F113 + Q118   W167 + T459   L219 + A263   Q299 + Q385       G7 + G423   G50 + Q256   F113 + Q125   W167 + P473   L219 + V264   Q299 + K391       G7 + T444   G50 + A263   F113 + G133   W167 + C474   L219 + Y267   Q299 + K393       G7 + A445   G50 + V264   F113 + T134   W167 + G476   L219 + N270   Q299 + Q395       G7 + Q449   G50 + Y267   F113 + W140   W167 + G477   L219 + G273   Q299 + A420       G7 + T459   G50 + N270   F113 + G142   W167 + K484   L219 + S280   Q299 + G423       G7 + P473   G50 + G273   F113 + G149   R171 + Q172   L219 + T285   Q299 + T444       G7 + C474   G50 + S280   F113 + T165   R171 + L173   L219 + M286   Q299 + A445       G7 + G476   G50 + T285   F113 + W167   R171 + A174   L219 + F289   Q299 + Q449       G7 + G477   G50 + M286   F113 + R171   R171 + G184   L219 + V291   Q299 + T459       G7 + K484   G50 + F289   F113 + Q172   R171 + T193   L219 + Q299   Q299 + P473       Q11 + N16   G50 + V291   F113 + L173   R171 + N195   L219 + R320   Q299 + C474       Q11 + V17   G50 + Q299   F113 + A174   R171 + A204   L219 + H321   Q299 + G476       Q11 + Q32   G50 + R320   F113 + G184   R171 + V206   L219 + S323   Q299 + G477       Q11 + A37   G50 + H321   F113 + T193   R171 + P211   L219 + H324   Q299 + K484       Q11 + T40   G50 + S323   F113 + N195   R171 + I214   L219 + F328   R320 + H321       Q11 + P45   G50 + H324   F113 + A204   R171 + V215   L219 + T334   R320 + S323       Q11 + W48   G50 + F328   F113 + V206   R171 + L217   L219 + D337   R320 + H324       Q11 + G50   G50 + T334   F113 + P211   R171 + L219   L219 + Q345   R320 + F328       Q11 + T51   G50 + D337   F113 + I214   R171 + L235   L219 + G346   R320 + T334       Q11 + N54   G50 + Q345   F113 + V215   R171 + V238   L219 + G348   R320 + D337       Q11 + V56   G50 + G346   F113 + L217   R171 + M246   L219 + T355   R320 + Q345       Q11 + K72   G50 + G348   F113 + L219   R171 + M248   L219 + S376   R320 + G346       Q11 + R87   G50 + T355   F113 + L235   R171 + L250   L219 + D377   R320 + G348       Q11 + Q98   G50 + S376   F113 + V238   R171 + G255   L219 + D379   R320 + T355       Q11 + M105   G50 + D377   F113 + M246   R171 + Q256   L219 + Y382   R320 + S376       Q11 + G109   G50 + D379   F113 + M248   R171 + A263   L219 + S383   R320 + D377       Q11 + F113   G50 + Y382   F113 + L250   R171 + V264   L219 + Q385   R320 + D379       Q11 + R116   G50 + S383   F113 + G255   R171 + Y267   L219 + K391   R320 + Y382       Q11 + Q118   G50 + Q385   F113 + Q256   R171 + N270   L219 + K393   R320 + S383       Q11 + Q125   G50 + K391   F113 + A263   R171 + G273   L219 + Q395   R320 + Q385       Q11 + G133   G50 + K393   F113 + V264   R171 + S280   L219 + A420   R320 + K391       Q11 + T134   G50 + Q395   F113 + Y267   R171 + T285   L219 + G423   R320 + K393       Q11 + W140   G50 + A420   F113 + N270   R171 + M286   L219 + T444   R320 + Q395       Q11 + G142   G50 + G423   F113 + G273   R171 + F289   L219 + A445   R320 + A420       Q11 + G149   G50 + T444   F113 + S280   R171 + V291   L219 + Q449   R320 + G423       Q11 + T165   G50 + A445   F113 + T285   R171 + Q299   L219 + T459   R320 + T444       Q11 + W167   G50 + Q449   F113 + M286   R171 + R320   L219 + P473   R320 + A445       Q11 + R171   G50 + T459   F113 + F289   R171 + H321   L219 + C474   R320 + Q449       Q11 + Q172   G50 + P473   F113 + V291   R171 + S323   L219 + G476   R320 + T459       Q11 + L173   G50 + C474   F113 + Q299   R171 + H324   L219 + G477   R320 + P473       Q11 + A174   G50 + G476   F113 + R320   R171 + F328   L219 + K484   R320 + C474       Q11 + G184   G50 + G477   F113 + H321   R171 + T334   L235 + V238   R320 + G476       Q11 + T193   G50 + K484   F113 + S323   R171 + D337   L235 + M246   R320 + G477       Q11 + N195   T51 + N54   F113 + H324   R171 + Q345   L235 + M248   R320 + K484       Q11 + A204   T51 + V56   F113 + F328   R171 + G346   L235 + L250   H321 + S323       Q11 + V206   T51 + K72   F113 + T334   R171 + G348   L235 + G255   H321 + H324       Q11 + P211   T51 + R87   F113 + D337   R171 + T355   L235 + Q256   H321 + F328       Q11 + I214   T51 + Q98   F113 + Q345   R171 + S376   L235 + A263   H321 + T334       Q11 + V215   T51 + M105   F113 + G346   R171 + D377   L235 + V264   H321 + D337       Q11 + L217   T51 + G109   F113 + G348   R171 + D379   L235 + Y267   H321 + Q345       Q11 + L219   T51 + F113   F113 + T355   R171 + Y382   L235 + N270   H321 + G346       Q11 + L235   T51 + R116   F113 + S376   R171 + S383   L235 + G273   H321 + G348       Q11 + V238   T51 + Q118   F113 + D377   R171 + Q385   L235 + S280   H321 + T355       Q11 + M246   T51 + Q125   F113 + D379   R171 + K391   L235 + T285   H321 + S376       Q11 + M248   T51 + G133   F113 + Y382   R171 + K393   L235 + M286   H321 + D377       Q11 + L250   T51 + T134   F113 + S383   R171 + Q395   L235 + F289   H321 + D379       Q11 + G255   T51 + W140   F113 + Q385   R171 + A420   L235 + V291   H321 + Y382       Q11 + Q256   T51 + G142   F113 + K391   R171 + G423   L235 + Q299   H321 + S383       Q11 + A263   T51 + G149   F113 + K393   R171 + T444   L235 + R320   H321 + Q385       Q11 + V264   T51 + T165   F113 + Q395   R171 + A445   L235 + H321   H321 + K391       Q11 + Y267   T51 + W167   F113 + A420   R171 + Q449   L235 + S323   H321 + K393       Q11 + N270   T51 + R171   F113 + G423   R171 + T459   L235 + H324   H321 + Q395       Q11 + G273   T51 + Q172   F113 + T444   R171 + P473   L235 + F328   H321 + A420       Q11 + S280   T51 + L173   F113 + A445   R171 + C474   L235 + T334   H321 + G423       Q11 + T285   T51 + A174   F113 + Q449   R171 + G476   L235 + D337   H321 + T444       Q11 + M286   T51 + G184   F113 + T459   R171 + G477   L235 + Q345   H321 + A445       Q11 + F289   T51 + T193   F113 + P473   R171 + K484   L235 + G346   H321 + Q449       Q11 + V291   T51 + N195   F113 + C474   Q172 + L173   L235 + G348   H321 + T459       Q11 + Q299   T51 + A204   F113 + G476   Q172 + A174   L235 + T355   H321 + P473       Q11 + R320   T51 + V206   F113 + G477   Q172 + G184   L235 + S376   H321 + C474       Q11 + H321   T51 + P211   F113 + K484   Q172 + T193   L235 + D377   H321 + G476       Q11 + S323   T51 + I214   R116 + Q118   Q172 + N195   L235 + D379   H321 + G477       Q11 + H324   T51 + V215   R116 + Q125   Q172 + A204   L235 + Y382   H321 + K484       Q11 + F328   T51 + L217   R116 + G133   Q172 + V206   L235 + S383   S323 + H324       Q11 + T334   T51 + L219   R116 + T134   Q172 + P211   L235 + Q385   S323 + F328       Q11 + D337   T51 + L235   R116 + W140   Q172 + I214   L235 + K391   S323 + T334       Q11 + Q345   T51 + V238   R116 + G142   Q172 + V215   L235 + K393   S323 + D337       Q11 + G346   T51 + M246   R116 + G149   Q172 + L217   L235 + Q395   S323 + Q345       Q11 + G348   T51 + M248   R116 + T165   Q172 + L219   L235 + A420   S323 + G346       Q11 + T355   T51 + L250   R116 + W167   Q172 + L235   L235 + G423   S323 + G348       Q11 + S376   T51 + G255   R116 + R171   Q172 + V238   L235 + T444   S323 + T355       Q11 + D377   T51 + Q256   R116 + Q172   Q172 + M246   L235 + A445   S323 + S376       Q11 + D379   T51 + A263   R116 + L173   Q172 + M248   L235 + Q449   S323 + D377       Q11 + Y382   T51 + V264   R116 + A174   Q172 + L250   L235 + T459   S323 + D379       Q11 + S383   T51 + Y267   R116 + G184   Q172 + G255   L235 + P473   S323 + Y382       Q11 + Q385   T51 + N270   R116 + T193   Q172 + Q256   L235 + C474   S323 + S383       Q11 + K391   T51 + G273   R116 + N195   Q172 + A263   L235 + G476   S323 + Q385       Q11 + K393   T51 + S280   R116 + A204   Q172 + V264   L235 + G477   S323 + K391       Q11 + Q395   T51 + T285   R116 + V206   Q172 + Y267   L235 + K484   S323 + K393       Q11 + A420   T51 + M286   R116 + P211   Q172 + N270   V238 + M246   S323 + Q395       Q11 + G423   T51 + F289   R116 + I214   Q172 + G273   V238 + M248   S323 + A420       Q11 + T444   T51 + V291   R116 + V215   Q172 + S280   V238 + L250   S323 + G423       Q11 + A445   T51 + Q299   R116 + L217   Q172 + T285   V238 + G255   S323 + T444       Q11 + Q449   T51 + R320   R116 + L219   Q172 + M286   V238 + Q256   S323 + A445       Q11 + T459   T51 + H321   R116 + L235   Q172 + F289   V238 + A263   S323 + Q449       Q11 + P473   T51 + S323   R116 + V238   Q172 + V291   V238 + V264   S323 + T459       Q11 + C474   T51 + H324   R116 + M246   Q172 + Q299   V238 + Y267   S323 + P473       Q11 + G476   T51 + F328   R116 + M248   Q172 + R320   V238 + N270   S323 + C474       Q11 + G477   T51 + T334   R116 + L250   Q172 + H321   V238 + G273   S323 + G476       Q11 + K484   T51 + D337   R116 + G255   Q172 + S323   V238 + S280   S323 + G477       N16 + V17   T51 + Q345   R116 + Q256   Q172 + H324   V238 + T285   S323 + K484       N16 + Q32   T51 + G346   R116 + A263   Q172 + F328   V238 + M286   H324 + F328       N16 + A37   T51 + G348   R116 + V264   Q172 + T334   V238 + F289   H324 + T334       N16 + T40   T51 + T355   R116 + Y267   Q172 + D337   V238 + V291   H324 + D337       N16 + P45   T51 + S376   R116 + N270   Q172 + Q345   V238 + Q299   H324 + Q345       N16 + W48   T51 + D377   R116 + G273   Q172 + G346   V238 + R320   H324 + G346       N16 + G50   T51 + D379   R116 + S280   Q172 + G348   V238 + H321   H324 + G348       N16 + T51   T51 + Y382   R116 + T285   Q172 + T355   V238 + S323   H324 + T355       N16 + N54   T51 + S383   R116 + M286   Q172 + S376   V238 + H324   H324 + S376       N16 + V56   T51 + Q385   R116 + F289   Q172 + D377   V238 + F328   H324 + D377       N16 + K72   T51 + K391   R116 + V291   Q172 + D379   V238 + T334   H324 + D379       N16 + R87   T51 + K393   R116 + Q299   Q172 + Y382   V238 + D337   H324 + Y382       N16 + Q98   T51 + Q395   R116 + R320   Q172 + S383   V238 + Q345   H324 + S383       N16 + M105   T51 + A420   R116 + H321   Q172 + Q385   V238 + G346   H324 + Q385       N16 + G109   T51 + G423   R116 + S323   Q172 + K391   V238 + G348   H324 + K391       N16 + F113   T51 + T444   R116 + H324   Q172 + K393   V238 + T355   H324 + K393       N16 + R116   T51 + A445   R116 + F328   Q172 + Q395   V238 + S376   H324 + Q395       N16 + Q118   T51 + Q449   R116 + T334   Q172 + A420   V238 + D377   H324 + A420       N16 + Q125   T51 + T459   R116 + D337   Q172 + G423   V238 + D379   H324 + G423       N16 + G133   T51 + P473   R116 + Q345   Q172 + T444   V238 + Y382   H324 + T444       N16 + T134   T51 + C474   R116 + G346   Q172 + A445   V238 + S383   H324 + A445       N16 + W140   T51 + G476   R116 + G348   Q172 + Q449   V238 + Q385   H324 + Q449       N16 + G142   T51 + G477   R116 + T355   Q172 + T459   V238 + K391   H324 + T459       N16 + G149   T51 + K484   R116 + S376   Q172 + P473   V238 + K393   H324 + P473       N16 + T165   N54 + V56   R116 + D377   Q172 + C474   V238 + Q395   H324 + C474       N16 + W167   N54 + K72   R116 + D379   Q172 + G476   V238 + A420   H324 + G476       N16 + R171   N54 + R87   R116 + Y382   Q172 + G477   V238 + G423   H324 + G477       N16 + Q172   N54 + Q98   R116 + S383   Q172 + K484   V238 + T444   H324 + K484       N16 + L173   N54 + M105   R116 + Q385   L173 + A174   V238 + A445   F328 + T334       N16 + A174   N54 + G109   R116 + K391   L173 + G184   V238 + Q449   F328 + D337       N16 + G184   N54 + F113   R116 + K393   L173 + T193   V238 + T459   F328 + Q345       N16 + T193   N54 + R116   R116 + Q395   L173 + N195   V238 + P473   F328 + G346       N16 + N195   N54 + Q118   R116 + A420   L173 + A204   V238 + C474   F328 + G348       N16 + A204   N54 + Q125   R116 + G423   L173 + V206   V238 + G476   F328 + T355       N16 + V206   N54 + G133   R116 + T444   L173 + P211   V238 + G477   F328 + S376       N16 + P211   N54 + T134   R116 + A445   L173 + I214   V238 + K484   F328 + D377       N16 + I214   N54 + W140   R116 + Q449   L173 + V215   M246 + M248   F328 + D379       N16 + V215   N54 + G142   R116 + T459   L173 + L217   M246 + L250   F328 + Y382       N16 + L217   N54 + G149   R116 + P473   L173 + L219   M246 + G255   F328 + S383       N16 + L219   N54 + T165   R116 + C474   L173 + L235   M246 + Q256   F328 + Q385       N16 + L235   N54 + W167   R116 + G476   L173 + V238   M246 + A263   F328 + K391       N16 + V238   N54 + R171   R116 + G477   L173 + M246   M246 + V264   F328 + K393       N16 + M246   N54 + Q172   R116 + K484   L173 + M248   M246 + Y267   F328 + Q395       N16 + M248   N54 + L173   Q118 + Q125   L173 + L250   M246 + N270   F328 + A420       N16 + L250   N54 + A174   Q118 + G133   L173 + G255   M246 + G273   F328 + G423       N16 + G255   N54 + G184   Q118 + T134   L173 + Q256   M246 + S280   F328 + T444       N16 + Q256   N54 + T193   Q118 + W140   L173 + A263   M246 + T285   F328 + A445       N16 + A263   N54 + N195   Q118 + G142   L173 + V264   M246 + M286   F328 + Q449       N16 + V264   N54 + A204   Q118 + G149   L173 + Y267   M246 + F289   F328 + T459       N16 + Y267   N54 + V206   Q118 + T165   L173 + N270   M246 + V291   F328 + P473       N16 + N270   N54 + P211   Q118 + W167   L173 + G273   M246 + Q299   F328 + C474       N16 + G273   N54 + I214   Q118 + R171   L173 + S280   M246 + R320   F328 + G476       N16 + S280   N54 + V215   Q118 + Q172   L173 + T285   M246 + H321   F328 + G477       N16 + T285   N54 + L217   Q118 + L173   L173 + M286   M246 + S323   F328 + K484       N16 + M286   N54 + L219   Q118 + A174   L173 + F289   M246 + H324   T334 + D337       N16 + F289   N54 + L235   Q118 + G184   L173 + V291   M246 + F328   T334 + Q345       N16 + V291   N54 + V238   Q118 + T193   L173 + Q299   M246 + T334   T334 + G346       N16 + Q299   N54 + M246   Q118 + N195   L173 + R320   M246 + D337   T334 + G348       N16 + R320   N54 + M248   Q118 + A204   L173 + H321   M246 + Q345   T334 + T355       N16 + H321   N54 + L250   Q118 + V206   L173 + S323   M246 + G346   T334 + S376       N16 + S323   N54 + G255   Q118 + P211   L173 + H324   M246 + G348   T334 + D377       N16 + H324   N54 + Q256   Q118 + I214   L173 + F328   M246 + T355   T334 + D379       N16 + F328   N54 + A263   Q118 + V215   L173 + T334   M246 + S376   T334 + Y382       N16 + T334   N54 + V264   Q118 + L217   L173 + D337   M246 + D377   T334 + S383       N16 + D337   N54 + Y267   Q118 + L219   L173 + Q345   M246 + D379   T334 + Q385       N16 + Q345   N54 + N270   Q118 + L235   L173 + G346   M246 + Y382   T334 + K391       N16 + G346   N54 + G273   Q118 + V238   L173 + G348   M246 + S383   T334 + K393       N16 + G348   N54 + S280   Q118 + M246   L173 + T355   M246 + Q385   T334 + Q395       N16 + T355   N54 + T285   Q118 + M248   L173 + S376   M246 + K391   T334 + A420       N16 + S376   N54 + M286   Q118 + L250   L173 + D377   M246 + K393   T334 + G423       N16 + D377   N54 + F289   Q118 + G255   L173 + D379   M246 + Q395   T334 + T444       N16 + D379   N54 + V291   Q118 + Q256   L173 + Y382   M246 + A420   T334 + A445       N16 + Y382   N54 + Q299   Q118 + A263   L173 + S383   M246 + G423   T334 + Q449       N16 + S383   N54 + R320   Q118 + V264   L173 + Q385   M246 + T444   T334 + T459       N16 + Q385   N54 + H321   Q118 + Y267   L173 + K391   M246 + A445   T334 + P473       N16 + K391   N54 + S323   Q118 + N270   L173 + K393   M246 + Q449   T334 + C474       N16 + K393   N54 + H324   Q118 + G273   L173 + Q395   M246 + T459   T334 + G476       N16 + Q395   N54 + F328   Q118 + S280   L173 + A420   M246 + P473   T334 + G477       N16 + A420   N54 + T334   Q118 + T285   L173 + G423   M246 + C474   T334 + K484       N16 + G423   N54 + D337   Q118 + M286   L173 + T444   M246 + G476   D337 + Q345       N16 + T444   N54 + Q345   Q118 + F289   L173 + A445   M246 + G477   D337 + G346       N16 + A445   N54 + G346   Q118 + V291   L173 + Q449   M246 + K484   D337 + G348       N16 + Q449   N54 + G348   Q118 + Q299   L173 + T459   M248 + L250   D337 + T355       N16 + T459   N54 + T355   Q118 + R320   L173 + P473   M248 + G255   D337 + S376       N16 + P473   N54 + S376   Q118 + H321   L173 + C474   M248 + Q256   D337 + D377       N16 + C474   N54 + D377   Q118 + S323   L173 + G476   M248 + A263   D337 + D379       N16 + G476   N54 + D379   Q118 + H324   L173 + G477   M248 + V264   D337 + Y382       N16 + G477   N54 + Y382   Q118 + F328   L173 + K484   M248 + Y267   D337 + S383       N16 + K484   N54 + S383   Q118 + T334   A174 + G184   M248 + N270   D337 + Q385       V17 + Q32   N54 + Q385   Q118 + D337   A174 + T193   M248 + G273   D337 + K391       V17 + A37   N54 + K391   Q118 + Q345   A174 + N195   M248 + S280   D337 + K393       V17 + T40   N54 + K393   Q118 + G346   A174 + A204   M248 + T285   D337 + Q395       V17 + P45   N54 + Q395   Q118 + G348   A174 + V206   M248 + M286   D337 + A420       V17 + W48   N54 + A420   Q118 + T355   A174 + P211   M248 + F289   D337 + G423       V17 + G50   N54 + G423   Q118 + S376   A174 + I214   M248 + V291   D337 + T444       V17 + T51   N54 + T444   Q118 + D377   A174 + V215   M248 + Q299   D337 + A445       V17 + N54   N54 + A445   Q118 + D379   A174 + L217   M248 + R320   D337 + Q449       V17 + V56   N54 + Q449   Q118 + Y382   A174 + L219   M248 + H321   D337 + T459       V17 + K72   N54 + T459   Q118 + S383   A174 + L235   M248 + S323   D337 + P473       V17 + R87   N54 + P473   Q118 + Q385   A174 + V238   M248 + H324   D337 + C474       V17 + Q98   N54 + C474   Q118 + K391   A174 + M246   M248 + F328   D337 + G476       V17 + M105   N54 + G476   Q118 + K393   A174 + M248   M248 + T334   D337 + G477       V17 + G109   N54 + G477   Q118 + Q395   A174 + L250   M248 + D337   D337 + K484       V17 + F113   N54 + K484   Q118 + A420   A174 + G255   M248 + Q345   Q345 + G346       V17 + R116   V56 + K72   Q118 + G423   A174 + Q256   M248 + G346   Q345 + G348       V17 + Q118   V56 + R87   Q118 + T444   A174 + A263   M248 + G348   Q345 + T355       V17 + Q125   V56 + Q98   Q118 + A445   A174 + V264   M248 + T355   Q345 + S376       V17 + G133   V56 + M105   Q118 + Q449   A174 + Y267   M248 + S376   Q345 + D377       V17 + T134   V56 + G109   Q118 + T459   A174 + N270   M248 + D377   Q345 + D379       V17 + W140   V56 + F113   Q118 + P473   A174 + G273   M248 + D379   Q345 + Y382       V17 + G142   V56 + R116   Q118 + C474   A174 + S280   M248 + Y382   Q345 + S383       V17 + G149   V56 + Q118   Q118 + G476   A174 + T285   M248 + S383   Q345 + Q385       V17 + T165   V56 + Q125   Q118 + G477   A174 + M286   M248 + Q385   Q345 + K391       V17 + W167   V56 + G133   Q118 + K484   A174 + F289   M248 + K391   Q345 + K393       V17 + R171   V56 + T134   Q125 + G133   A174 + V291   M248 + K393   Q345 + Q395       V17 + Q172   V56 + W140   Q125 + T134   A174 + Q299   M248 + Q395   Q345 + A420       V17 + L173   V56 + G142   Q125 + W140   A174 + R320   M248 + A420   Q345 + G423       V17 + A174   V56 + G149   Q125 + G142   A174 + H321   M248 + G423   Q345 + T444       V17 + G184   V56 + T165   Q125 + G149   A174 + S323   M248 + T444   Q345 + A445       V17 + T193   V56 + W167   Q125 + T165   A174 + H324   M248 + A445   Q345 + Q449       V17 + N195   V56 + R171   Q125 + W167   A174 + F328   M248 + Q449   Q345 + T459       V17 + A204   V56 + Q172   Q125 + R171   A174 + T334   M248 + T459   Q345 + P473       V17 + V206   V56 + L173   Q125 + Q172   A174 + D337   M248 + P473   Q345 + C474       V17 + P211   V56 + A174   Q125 + L173   A174 + Q345   M248 + C474   Q345 + G476       V17 + I214   V56 + G184   Q125 + A174   A174 + G346   M248 + G476   Q345 + G477       V17 + V215   V56 + T193   Q125 + G184   A174 + G348   M248 + G477   Q345 + K484       V17 + L217   V56 + N195   Q125 + T193   A174 + T355   M248 + K484   G346 + G348       V17 + L219   V56 + A204   Q125 + N195   A174 + S376   L250 + G255   G346 + T355       V17 + L235   V56 + V206   Q125 + A204   A174 + D377   L250 + Q256   G346 + S376       V17 + V238   V56 + P211   Q125 + V206   A174 + D379   L250 + A263   G346 + D377       V17 + M246   V56 + I214   Q125 + P211   A174 + Y382   L250 + V264   G346 + D379       V17 + M248   V56 + V215   Q125 + I214   A174 + S383   L250 + Y267   G346 + Y382       V17 + L250   V56 + L217   Q125 + V215   A174 + Q385   L250 + N270   G346 + S383       V17 + G255   V56 + L219   Q125 + L217   A174 + K391   L250 + G273   G346 + Q385       V17 + Q256   V56 + L235   Q125 + L219   A174 + K393   L250 + S280   G346 + K391       V17 + A263   V56 + V238   Q125 + L235   A174 + Q395   L250 + T285   G346 + K393       V17 + V264   V56 + M246   Q125 + V238   A174 + A420   L250 + M286   G346 + Q395       V17 + Y267   V56 + M248   Q125 + M246   A174 + G423   L250 + F289   G346 + A420       V17 + N270   V56 + L250   Q125 + M248   A174 + T444   L250 + V291   G346 + G423       V17 + G273   V56 + G255   Q125 + L250   A174 + A445   L250 + Q299   G346 + T444       V17 + S280   V56 + Q256   Q125 + G255   A174 + Q449   L250 + R320   G346 + A445       V17 + T285   V56 + A263   Q125 + Q256   A174 + T459   L250 + H321   G346 + Q449       V17 + M286   V56 + V264   Q125 + A263   A174 + P473   L250 + S323   G346 + T459       V17 + F289   V56 + Y267   Q125 + V264   A174 + C474   L250 + H324   G346 + P473       V17 + V291   V56 + N270   Q125 + Y267   A174 + G476   L250 + F328   G346 + C474       V17 + Q299   V56 + G273   Q125 + N270   A174 + G477   L250 + T334   G346 + G476       V17 + R320   V56 + S280   Q125 + G273   A174 + K484   L250 + D337   G346 + G477       V17 + H321   V56 + T285   Q125 + S280   G184 + T193   L250 + Q345   G346 + K484       V17 + S323   V56 + M286   Q125 + T285   G184 + N195   L250 + G346   G348 + T355       V17 + H324   V56 + F289   Q125 + M286   G184 + A204   L250 + G348   G348 + S376       V17 + F328   V56 + V291   Q125 + F289   G184 + V206   L250 + T355   G348 + D377       V17 + T334   V56 + Q299   Q125 + V291   G184 + P211   L250 + S376   G348 + D379       V17 + D337   V56 + R320   Q125 + Q299   G184 + I214   L250 + D377   G348 + Y382       V17 + Q345   V56 + H321   Q125 + R320   G184 + V215   L250 + D379   G348 + S383       V17 + G346   V56 + S323   Q125 + H321   G184 + L217   L250 + Y382   G348 + Q385       V17 + G348   V56 + H324   Q125 + S323   G184 + L219   L250 + S383   G348 + K391       V17 + T355   V56 + F328   Q125 + H324   G184 + L235   L250 + Q385   G348 + K393       V17 + S376   V56 + T334   Q125 + F328   G184 + V238   L250 + K391   G348 + Q395       V17 + D377   V56 + D337   Q125 + T334   G184 + M246   L250 + K393   G348 + A420       V17 + D379   V56 + Q345   Q125 + D337   G184 + M248   L250 + Q395   G348 + G423       V17 + Y382   V56 + G346   Q125 + Q345   G184 + L250   L250 + A420   G348 + T444       V17 + S383   V56 + G348   Q125 + G346   G184 + G255   L250 + G423   G348 + A445       V17 + Q385   V56 + T355   Q125 + G348   G184 + Q256   L250 + T444   G348 + Q449       V17 + K391   V56 + S376   Q125 + T355   G184 + A263   L250 + A445   G348 + T459       V17 + K393   V56 + D377   Q125 + S376   G184 + V264   L250 + Q449   G348 + P473       V17 + Q395   V56 + D379   Q125 + D377   G184 + Y267   L250 + T459   G348 + C474       V17 + A420   V56 + Y382   Q125 + D379   G184 + N270   L250 + P473   G348 + G476       V17 + G423   V56 + S383   Q125 + Y382   G184 + G273   L250 + C474   G348 + G477       V17 + T444   V56 + Q385   Q125 + S383   G184 + S280   L250 + G476   G348 + K484       V17 + A445   V56 + K391   Q125 + Q385   G184 + T285   L250 + G477   T355 + S376       V17 + Q449   V56 + K393   Q125 + K391   G184 + M286   L250 + K484   T355 + D377       V17 + T459   V56 + Q395   Q125 + K393   G184 + F289   G255 + Q256   T355 + D379       V17 + P473   V56 + A420   Q125 + Q395   G184 + V291   G255 + A263   T355 + Y382       V17 + C474   V56 + G423   Q125 + A420   G184 + Q299   G255 + V264   T355 + S383       V17 + G476   V56 + T444   Q125 + G423   G184 + R320   G255 + Y267   T355 + Q385       V17 + G477   V56 + A445   Q125 + T444   G184 + H321   G255 + N270   T355 + K391       V17 + K484   V56 + Q449   Q125 + A445   G184 + S323   G255 + G273   T355 + K393       Q32 + A37   V56 + T459   Q125 + Q449   G184 + H324   G255 + S280   T355 + Q395       Q32 + T40   V56 + P473   Q125 + T459   G184 + F328   G255 + T285   T355 + A420       Q32 + P45   V56 + C474   Q125 + P473   G184 + T334   G255 + M286   T355 + G423       Q32 + W48   V56 + G476   Q125 + C474   G184 + D337   G255 + F289   T355 + T444       Q32 + G50   V56 + G477   Q125 + G476   G184 + Q345   G255 + V291   T355 + A445       Q32 + T51   V56 + K484   Q125 + G477   G184 + G346   G255 + Q299   T355 + Q449       Q32 + N54   K72 + R87   Q125 + K484   G184 + G348   G255 + R320   T355 + T459       Q32 + V56   K72 + Q98   G133 + T134   G184 + T355   G255 + H321   T355 + P473       Q32 + K72   K72 + M105   G133 + W140   G184 + S376   G255 + S323   T355 + C474       Q32 + R87   K72 + G109   G133 + G142   G184 + D377   G255 + H324   T355 + G476       Q32 + Q98   K72 + F113   G133 + G149   G184 + D379   G255 + F328   T355 + G477       Q32 + M105   K72 + R116   G133 + T165   G184 + Y382   G255 + T334   T355 + K484       Q32 + G109   K72 + Q118   G133 + W167   G184 + S383   G255 + D337   S376 + D377       Q32 + F113   K72 + Q125   G133 + R171   G184 + Q385   G255 + Q345   S376 + D379       Q32 + R116   K72 + G133   G133 + Q172   G184 + K391   G255 + G346   S376 + Y382       Q32 + Q118   K72 + T134   G133 + L173   G184 + K393   G255 + G348   S376 + S383       Q32 + Q125   K72 + W140   G133 + A174   G184 + Q395   G255 + T355   S376 + Q385       Q32 + G133   K72 + G142   G133 + G184   G184 + A420   G255 + S376   S376 + K391       Q32 + T134   K72 + G149   G133 + T193   G184 + G423   G255 + D377   S376 + K393       Q32 + W140   K72 + T165   G133 + N195   G184 + T444   G255 + D379   S376 + Q395       Q32 + G142   K72 + W167   G133 + A204   G184 + A445   G255 + Y382   S376 + A420       Q32 + G149   K72 + R171   G133 + V206   G184 + Q449   G255 + S383   S376 + G423       Q32 + T165   K72 + Q172   G133 + P211   G184 + T459   G255 + Q385   S376 + T444       Q32 + W167   K72 + L173   G133 + I214   G184 + P473   G255 + K391   S376 + A445       Q32 + R171   K72 + A174   G133 + V215   G184 + C474   G255 + K393   S376 + Q449       Q32 + Q172   K72 + G184   G133 + L217   G184 + G476   G255 + Q395   S376 + T459       Q32 + L173   K72 + T193   G133 + L219   G184 + G477   G255 + A420   S376 + P473       Q32 + A174   K72 + N195   G133 + L235   G184 + K484   G255 + G423   S376 + C474       Q32 + G184   K72 + A204   G133 + V238   T193 + N195   G255 + T444   S376 + G476       Q32 + T193   K72 + V206   G133 + M246   T193 + A204   G255 + A445   S376 + G477       Q32 + N195   K72 + P211   G133 + M248   T193 + V206   G255 + Q449   S376 + K484       Q32 + A204   K72 + I214   G133 + L250   T193 + P211   G255 + T459   D377 + D379       Q32 + V206   K72 + V215   G133 + G255   T193 + I214   G255 + P473   D377 + Y382       Q32 + P211   K72 + L217   G133 + Q256   T193 + V215   G255 + C474   D377 + S383       Q32 + I214   K72 + L219   G133 + A263   T193 + L217   G255 + G476   D377 + Q385       Q32 + V215   K72 + L235   G133 + V264   T193 + L219   G255 + G477   D377 + K391       Q32 + L217   K72 + V238   G133 + Y267   T193 + L235   G255 + K484   D377 + K393       Q32 + L219   K72 + M246   G133 + N270   T193 + V238   Q256 + A263   D377 + Q395       Q32 + L235   K72 + M248   G133 + G273   T193 + M246   Q256 + V264   D377 + A420       Q32 + V238   K72 + L250   G133 + S280   T193 + M248   Q256 + Y267   D377 + G423       Q32 + M246   K72 + G255   G133 + T285   T193 + L250   Q256 + N270   D377 + T444       Q32 + M248   K72 + Q256   G133 + M286   T193 + G255   Q256 + G273   D377 + A445       Q32 + L250   K72 + A263   G133 + F289   T193 + Q256   Q256 + S280   D377 + Q449       Q32 + G255   K72 + V264   G133 + V291   T193 + A263   Q256 + T285   D377 + T459       Q32 + Q256   K72 + Y267   G133 + Q299   T193 + V264   Q256 + M286   D377 + P473       Q32 + A263   K72 + N270   G133 + R320   T193 + Y267   Q256 + F289   D377 + C474       Q32 + V264   K72 + G273   G133 + H321   T193 + N270   Q256 + V291   D377 + G476       Q32 + Y267   K72 + S280   G133 + S323   T193 + G273   Q256 + Q299   D377 + G477       Q32 + N270   K72 + T285   G133 + H324   T193 + S280   Q256 + R320   D377 + K484       Q32 + G273   K72 + M286   G133 + F328   T193 + T285   Q256 + H321   D379 + Y382       Q32 + S280   K72 + F289   G133 + T334   T193 + M286   Q256 + S323   D379 + S383       Q32 + T285   K72 + V291   G133 + D337   T193 + F289   Q256 + H324   D379 + Q385       Q32 + M286   K72 + Q299   G133 + Q345   T193 + V291   Q256 + F328   D379 + K391       Q32 + F289   K72 + R320   G133 + G346   T193 + Q299   Q256 + T334   D379 + K393       Q32 + V291   K72 + H321   G133 + G348   T193 + R320   Q256 + D337   D379 + Q395       Q32 + Q299   K72 + S323   G133 + T355   T193 + H321   Q256 + Q345   D379 + A420       Q32 + R320   K72 + H324   G133 + S376   T193 + S323   Q256 + G346   D379 + G423       Q32 + H321   K72 + F328   G133 + D377   T193 + H324   Q256 + G348   D379 + T444       Q32 + S323   K72 + T334   G133 + D379   T193 + F328   Q256 + T355   D379 + A445       Q32 + H324   K72 + D337   G133 + Y382   T193 + T334   Q256 + S376   D379 + Q449       Q32 + F328   K72 + Q345   G133 + S383   T193 + D337   Q256 + D377   D379 + T459       Q32 + T334   K72 + G346   G133 + Q385   T193 + Q345   Q256 + D379   D379 + P473       Q32 + D337   K72 + G348   G133 + K391   T193 + G346   Q256 + Y382   D379 + C474       Q32 + Q345   K72 + T355   G133 + K393   T193 + G348   Q256 + S383   D379 + G476       Q32 + G346   K72 + S376   G133 + Q395   T193 + T355   Q256 + Q385   D379 + G477       Q32 + G348   K72 + D377   G133 + A420   T193 + S376   Q256 + K391   D379 + K484       Q32 + T355   K72 + D379   G133 + G423   T193 + D377   Q256 + K393   Y382 + S383       Q32 + S376   K72 + Y382   G133 + T444   T193 + D379   Q256 + Q395   Y382 + Q385       Q32 + D377   K72 + S383   G133 + A445   T193 + Y382   Q256 + A420   Y382 + K391       Q32 + D379   K72 + Q385   G133 + Q449   T193 + S383   Q256 + G423   Y382 + K393       Q32 + Y382   K72 + K391   G133 + T459   T193 + Q385   Q256 + T444   Y382 + Q395       Q32 + S383   K72 + K393   G133 + P473   T193 + K391   Q256 + A445   Y382 + A420       Q32 + Q385   K72 + Q395   G133 + C474   T193 + K393   Q256 + Q449   Y382 + G423       Q32 + K391   K72 + A420   G133 + G476   T193 + Q395   Q256 + T459   Y382 + T444       Q32 + K393   K72 + G423   G133 + G477   T193 + A420   Q256 + P473   Y382 + A445       Q32 + Q395   K72 + T444   G133 + K484   T193 + G423   Q256 + C474   Y382 + Q449       Q32 + A420   K72 + A445   T134 + W140   T193 + T444   Q256 + G476   Y382 + T459       Q32 + G423   K72 + Q449   T134 + G142   T193 + A445   Q256 + G477   Y382 + P473       Q32 + T444   K72 + T459   T134 + G149   T193 + Q449   Q256 + K484   Y382 + C474       Q32 + A445   K72 + P473   T134 + T165   T193 + T459   A263 + V264   Y382 + G476       Q32 + Q449   K72 + C474   T134 + W167   T193 + P473   A263 + Y267   Y382 + G477       Q32 + T459   K72 + G476   T134 + R171   T193 + C474   A263 + N270   Y382 + K484       Q32 + P473   K72 + G477   T134 + Q172   T193 + G476   A263 + G273   S383 + Q385       Q32 + C474   K72 + K484   T134 + L173   T193 + G477   A263 + S280   S383 + K391       Q32 + G476   R87 + Q98   T134 + A174   T193 + K484   A263 + T285   S383 + K393       Q32 + G477   R87 + M105   T134 + G184   N195 + A204   A263 + M286   S383 + Q395       Q32 + K484   R87 + G109   T134 + T193   N195 + V206   A263 + F289   S383 + A420       A37 + T40   R87 + F113   T134 + N195   N195 + P211   A263 + V291   S383 + G423       A37 + P45   R87 + R116   T134 + A204   N195 + I214   A263 + Q299   S383 + T444       A37 + W48   R87 + Q118   T134 + V206   N195 + V215   A263 + R320   S383 + A445       A37 + G50   R87 + Q125   T134 + P211   N195 + L217   A263 + H321   S383 + Q449       A37 + T51   R87 + G133   T134 + I214   N195 + L219   A263 + S323   S383 + T459       A37 + N54   R87 + T134   T134 + V215   N195 + L235   A263 + H324   S383 + P473       A37 + V56   R87 + W140   T134 + L217   N195 + V238   A263 + F328   S383 + C474       A37 + K72   R87 + G142   T134 + L219   N195 + M246   A263 + T334   S383 + G476       A37 + R87   R87 + G149   T134 + L235   N195 + M248   A263 + D337   S383 + G477       A37 + Q98   R87 + T165   T134 + V238   N195 + L250   A263 + Q345   S383 + K484       A37 + M105   R87 + W167   T134 + M246   N195 + G255   A263 + G346   Q385 + K391       A37 + G109   R87 + R171   T134 + M248   N195 + Q256   A263 + G348   Q385 + K393       A37 + F113   R87 + Q172   T134 + L250   N195 + A263   A263 + T355   Q385 + Q395       A37 + R116   R87 + L173   T134 + G255   N195 + V264   A263 + S376   Q385 + A420       A37 + Q118   R87 + A174   T134 + Q256   N195 + Y267   A263 + D377   Q385 + G423       A37 + Q125   R87 + G184   T134 + A263   N195 + N270   A263 + D379   Q385 + T444       A37 + G133   R87 + T193   T134 + V264   N195 + G273   A263 + Y382   Q385 + A445       A37 + T134   R87 + N195   T134 + Y267   N195 + S280   A263 + S383   Q385 + Q449       A37 + W140   R87 + A204   T134 + N270   N195 + T285   A263 + Q385   Q385 + T459       A37 + G142   R87 + V206   T134 + G273   N195 + M286   A263 + K391   Q385 + P473       A37 + G149   R87 + P211   T134 + S280   N195 + F289   A263 + K393   Q385 + C474       A37 + T165   R87 + I214   T134 + T285   N195 + V291   A263 + Q395   Q385 + G476       A37 + W167   R87 + V215   T134 + M286   N195 + Q299   A263 + A420   Q385 + G477       A37 + R171   R87 + L217   T134 + F289   N195 + R320   A263 + G423   Q385 + K484       A37 + Q172   R87 + L219   T134 + V291   N195 + H321   A263 + T444   K391 + K393       A37 + L173   R87 + L235   T134 + Q299   N195 + S323   A263 + A445   K391 + Q395       A37 + A174   R87 + V238   T134 + R320   N195 + H324   A263 + Q449   K391 + A420       A37 + G184   R87 + M246   T134 + H321   N195 + F328   A263 + T459   K391 + G423       A37 + T193   R87 + M248   T134 + S323   N195 + T334   A263 + P473   K391 + T444       A37 + N195   R87 + L250   T134 + H324   N195 + D337   A263 + C474   K391 + A445       A37 + A204   R87 + G255   T134 + F328   N195 + Q345   A263 + G476   K391 + Q449       A37 + V206   R87 + Q256   T134 + T334   N195 + G346   A263 + G477   K391 + T459       A37 + P211   R87 + A263   T134 + D337   N195 + G348   A263 + K484   K391 + P473       A37 + I214   R87 + V264   T134 + Q345   N195 + T355   V264 + Y267   K391 + C474       A37 + V215   R87 + Y267   T134 + G346   N195 + S376   V264 + N270   K391 + G476       A37 + L217   H1 + Q169   T134 + G348   N195 + D377   V264 + G273   K391 + G477       A37 + L219   H1 + A186   T134 + T355   N195 + D379   V264 + S280   K391 + K484       A37 + L235   H1 + E190   T134 + S376   N195 + Y382   V264 + T285   K393 + Q395       A37 + V238   H1 + A225   T134 + D377   N195 + S383   V264 + M286   K393 + A420       A37 + M246   H1 + K242   T134 + D379   N195 + Q385   V264 + F289   K393 + G423       A37 + M248   H1 + S244   T134 + Y382   N195 + K391   V264 + V291   K393 + T444       A37 + L250   H1 + G258   T134 + S383   N195 + K393   V264 + Q299   K393 + A445       A37 + G255   H1 + N260   T134 + Q385   N195 + Q395   V264 + R320   K393 + Q449       A37 + Q256   H1 + K269   T134 + K391   N195 + A420   V264 + H321   K393 + T459       A37 + A263   H1 + W284   T134 + K393   N195 + G423   V264 + S323   K393 + P473       A37 + V264   H1 + Y295   T134 + Q395   N195 + T444   V264 + H324   K393 + C474       A37 + Y267   H1 + S304   T134 + A420   N195 + A445   V264 + F328   K393 + G476       A37 + N270   H1 + V326   T134 + G423   N195 + Q449   V264 + T334   K393 + G477       A37 + G273   T5 + Q169   T134 + T444   N195 + T459   V264 + D337   K393 + K484       A37 + S280   T5 + A186   T134 + A445   N195 + P473   V264 + Q345   Q395 + A420       A37 + T285   T5 + E190   T134 + Q449   N195 + C474   V264 + G346   Q395 + G423       A37 + M286   T5 + A225   T134 + T459   N195 + G476   V264 + G348   Q395 + T444       A37 + F289   T5 + K242   T134 + P473   N195 + G477   V264 + T355   Q395 + A445       A37 + V291   T5 + S244   T134 + C474   N195 + K484   V264 + S376   Q395 + Q449       A37 + Q299   T5 + G258   T134 + G476   A204 + V206   V264 + D377   Q395 + T459       A37 + R320   T5 + N260   T134 + G477   A204 + P211   V264 + D379   Q395 + P473       A37 + H321   T5 + K269   T134 + K484   A204 + I214   V264 + Y382   Q395 + C474       A37 + S323   T5 + W284   W140 + G142   A204 + V215   V264 + S383   Q395 + G476       A37 + H324   T5 + Y295   W140 + G149   A204 + L217   V264 + Q385   Q395 + G477       A37 + F328   T5 + S304   W140 + T165   A204 + L219   V264 + K391   Q395 + K484       A37 + T334   T5 + V326   W140 + W167   A204 + L235   V264 + K393   A420 + G423       A37 + D337   G7 + Q169   W140 + R171   A204 + V238   V264 + Q395   A420 + T444       A37 + Q345   G7 + A186   W140 + Q172   A204 + M246   V264 + A420   A420 + A445       A37 + G346   G7 + E190   W140 + L173   A204 + M248   V264 + G423   A420 + Q449       A37 + G348   G7 + A225   W140 + A174   A204 + L250   V264 + T444   A420 + T459       A37 + T355   G7 + K242   W140 + G184   A204 + G255   V264 + A445   A420 + P473       A37 + S376   G7 + S244   W140 + T193   A204 + Q256   V264 + Q449   A420 + C474       A37 + D377   G7 + G258   W140 + N195   A204 + A263   V264 + T459   A420 + G476       A37 + D379   G7 + N260   W140 + A204   A204 + V264   V264 + P473   A420 + G477       A37 + Y382   G7 + A265   W140 + V206   A204 + Y267   V264 + C474   A420 + K484       A37 + S383   G7 + K269   W140 + P211   A204 + N270   V264 + G476   G423 + T444       A37 + Q385   G7 + W284   W140 + I214   A204 + G273   V264 + G477   G423 + A445       A37 + K391   G7 + Y295   W140 + V215   A204 + S280   V264 + K484   G423 + Q449       A37 + K393   G7 + S304   W140 + L217   A204 + T285   Y267 + N270   G423 + T459       A37 + Q395   G7 + V326   W140 + L219   A204 + M286   Y267 + G273   G423 + P473       A37 + A420   Q11 + Q169   W140 + L235   A204 + F289   Y267 + S280   G423 + C474       A37 + G423   Q11 + A186   W140 + V238   A204 + V291   Y267 + T285   G423 + G476       A37 + T444   Q11 + E190   W140 + M246   A204 + Q299   Y267 + M286   G423 + G477       A37 + A445   Q11 + A225   W140 + M248   A204 + R320   Y267 + F289   G423 + K484       A37 + Q449   Q11 + K242   W140 + L250   A204 + H321   Y267 + V291   T444 + A445       A37 + T459   Q11 + S244   W140 + G255   A204 + S323   Y267 + Q299   T444 + Q449       A37 + P473   Q11 + G258   W140 + Q256   A204 + H324   Y267 + R320   T444 + T459       A37 + C474   Q11 + N260   W140 + A263   A204 + F328   Y267 + H321   T444 + P473       A37 + G476   Q11 + K269   W140 + V264   A204 + T334   Y267 + S323   T444 + C474       A37 + G477   Q11 + W284   W140 + Y267   A204 + D337   Y267 + H324   T444 + G476       A37 + K484   Q11 + Y295   W140 + N270   A204 + Q345   Y267 + F328   T444 + G477       T40 + P45   Q11 + S304   W140 + G273   A204 + G346   Y267 + T334   T444 + K484       T40 + W48   Q11 + V326   W140 + S280   A204 + G348   Y267 + D337   A445 + Q449       T40 + G50   N16 + Q169   W140 + T285   A204 + T355   Y267 + Q345   A445 + T459       T40 + T51   N16 + A186   W140 + M286   A204 + S376   Y267 + G346   A445 + P473       T40 + N54   N16 + E190   W140 + F289   A204 + D377   Y267 + G348   A445 + C474       T40 + V56   N16 + A225   W140 + V291   A204 + D379   Y267 + T355   A445 + G476       T40 + K72   N16 + K242   W140 + Q299   A204 + Y382   Y267 + S376   A445 + G477       T40 + R87   N16 + S244   W140 + R320   A204 + S383   Y267 + D377   A445 + K484       T40 + Q98   N16 + G258   W140 + H321   A204 + Q385   Y267 + D379   Q449 + T459       T40 + M105   N16 + N260   W140 + S323   A204 + K391   Y267 + Y382   Q449 + P473       T40 + G109   N16 + K269   W140 + H324   A204 + K393   Y267 + S383   Q449 + C474       T40 + F113   N16 + W284   W140 + F328   A204 + Q395   Y267 + Q385   Q449 + G476       T40 + R116   N16 + Y295   W140 + T334   A204 + A420   Y267 + K391   Q449 + G477       T40 + Q118   N16 + S304   W140 + D337   A204 + G423   Y267 + K393   Q449 + K484       T40 + Q125   N16 + V326   W140 + Q345   A204 + T444   Y267 + Q395   T459 + P473       T40 + G133   V17 + Q169   W140 + G346   A204 + A445   Y267 + A420   T459 + C474       T40 + T134   V17 + A186   W140 + G348   A204 + Q449   Y267 + G423   T459 + G476       T40 + W140   V17 + E190   W140 + T355   A204 + T459   Y267 + T444   T459 + G477       T40 + G142   V17 + A225   W140 + S376   A204 + P473   Y267 + A445   T459 + K484       T40 + G149   V17 + K242   W140 + D377   A204 + C474   Y267 + Q449   P473 + C474       T40 + T165   V17 + S244   W140 + D379   A204 + G476   Y267 + T459   P473 + G476       T40 + W167   V17 + G258   W140 + Y382   A204 + G477   Y267 + P473   P473 + G477       T40 + R171   V17 + N260   W140 + S383   A204 + K484   G109 + Q169   P473 + K484       T40 + Q172   V17 + K269   W140 + Q385   V206 + P211   G109 + A186   C474 + G476       T40 + L173   V17 + W284   W140 + K391   V206 + I214   G109 + E190   C474 + G477       T40 + Q169   V17 + Y295   N54 + Q169   K72 + Q169   G109 + A225   C474 + K484       T40 + A186   V17 + S304   N54 + A186   K72 + A186   G109 + K242   G476 + G477       T40 + E190   V17 + V326   N54 + E190   K72 + E190   G109 + S244   G476 + K484       T40 + A225   Q32 + Q169   N54 + A225   K72 + A225   G109 + G258   G477 + K484       T40 + K242   Q32 + A186   N54 + K242   K72 + K242   G109 + N260   G133 + Q169       T40 + S244   Q32 + E190   N54 + S244   K72 + S244   G109 + K269   G133 + A186       T40 + G258   Q32 + A225   N54 + G258   K72 + G258   G109 + W284   G133 + E190       T40 + N260   Q32 + K242   N54 + N260   K72 + N260   G109 + Y295   G133 + A225       T40 + K269   Q32 + S244   N54 + K269   K72 + K269   G109 + S304   G133 + K242       T40 + W284   Q32 + G258   N54 + W284   K72 + W284   G109 + V326   G133 + S244       T40 + Y295   Q32 + N260   N54 + Y295   K72 + Y295   F113 + Q169   G133 + G258       T40 + S304   Q32 + K269   N54 + S304   K72 + S304   F113 + A186   G133 + N260       T40 + V326   Q32 + W284   N54 + V326   K72 + V326   F113 + E190   G133 + K269       P45 + Q169   Q32 + Y295   V56 + Q169   R87 + Q169   F113 + A225   G133 + W284       P45 + A186   Q32 + S304   V56 + A186   R87 + A186   F113 + K242   G133 + Y295       P45 + E190   Q32 + V326   V56 + E190   R87 + E190   F113 + S244   G133 + S304       P45 + A225   A37 + Q169   V56 + A225   R87 + A225   F113 + G258   G133 + V326       P45 + K242   A37 + A186   V56 + K242   R87 + K242   F113 + N260   T134 + Q169       P45 + S244   A37 + E190   V56 + S244   R87 + S244   F113 + K269   T134 + A186       P45 + G258   A37 + A225   V56 + G258   R87 + G258   F113 + W284   T134 + E190       P45 + N260   A37 + K242   V56 + N260   R87 + N260   F113 + Y295   T134 + A225       P45 + K269   A37 + S244   V56 + K269   R87 + K269   F113 + S304   T134 + K242       P45 + W284   A37 + G258   V56 + W284   R87 + W284   F113 + V326   T134 + S244       P45 + Y295   A37 + N260   V56 + Y295   R87 + Y295   R116 + Q169   T134 + G258       P45 + S304   A37 + K269   V56 + S304   R87 + S304   R116 + A186   T134 + N260       P45 + V326   A37 + W284   V56 + V326   R87 + V326   R116 + E190   T134 + K269       W48 + Q169   A37 + Y295   Q169 + R171   Q98 + Q169   R116 + A225   T134 + W284       W48 + A186   A37 + S304   Q169 + Q172   Q98 + A186   R116 + K242   T134 + Y295       W48 + E190   A37 + V326   Q169 + L173   Q98 + E190   R116 + S244   T134 + S304       W48 + A225   G142 + Q169   Q169 + A174   Q98 + A225   R116 + G258   T134 + V326       W48 + K242   G142 + A186   Q169 + G184   Q98 + K242   R116 + N260   W140 + Q169       W48 + S244   G142 + E190   Q169 + A186   Q98 + S244   R116 + K269   W140 + A186       W48 + G258   G142 + A225   Q169 + E190   Q98 + G258   R116 + W284   W140 + E190       W48 + N260   G142 + K242   Q169 + T193   Q98 + N260   R116 + Y295   W140 + A225       W48 + K269   G142 + S244   Q169 + N195   Q98 + K269   R116 + S304   W140 + K242       W48 + W284   G142 + G258   Q169 + A204   Q98 + W284   R116 + V326   W140 + S244       W48 + Y295   G142 + N260   Q169 + V206   Q98 + Y295   Q118 + Q169   W140 + G258       W48 + S304   G142 + K269   Q169 + P211   Q98 + S304   Q118 + A186   W140 + N260       W48 + V326   G142 + W284   Q169 + I214   Q98 + V326   Q118 + E190   W140 + K269       G50 + Q169   G142 + Y295   Q169 + V215   M105 + Q169   Q118 + A225   W140 + W284       G50 + A186   G142 + S304   Q169 + L217   M105 + A186   Q118 + K242   W140 + Y295       G50 + E190   G142 + V326   Q169 + L219   M105 + E190   Q118 + S244   W140 + S304       G50 + A225   G149 + Q169   Q169 + A225   M105 + A225   Q118 + G258   W140 + V326       G50 + K242   G149 + A186   Q169 + L235   M105 + K242   Q118 + N260   E190 + L235       G50 + S244   G149 + E190   Q169 + V238   M105 + S244   Q118 + K269   A225 + V238       G50 + G258   G149 + A225   Q169 + K242   M105 + G258   Q118 + W284   A225 + K242       G50 + N260   G149 + K242   Q169 + S244   M105 + N260   Q118 + Y295   A225 + S244       G50 + K269   G149 + S244   Q169 + M246   M105 + K269   Q118 + S304   A225 + M246       G50 + W284   G149 + G258   Q169 + M248   M105 + W284   Q118 + V326   A225 + M248       G50 + Y295   G149 + N260   Q169 + L250   M105 + Y295   Q125 + Q169   A225 + L250       G50 + S304   G149 + K269   Q169 + G255   M105 + S304   Q125 + A186   A225 + G255       G50 + V326   G149 + W284   Q169 + Q256   M105 + V326   Q125 + E190   A225 + Q256       T51 + Q169   G149 + Y295   Q169 + G258   R171 + A186   Q125 + A225   A225 + G258       T51 + A186   G149 + S304   Q169 + N260   R171 + E190   Q125 + K242   A225 + N260       T51 + E190   G149 + V326   Q169 + A263   R171 + A225   Q125 + S244   A225 + A263       T51 + A225   T165 + Q169   Q169 + V264   R171 + K242   Q125 + G258   A225 + V264       T51 + K242   T165 + A186   Q169 + Y267   R171 + S244   Q125 + N260   A225 + Y267       T51 + S244   T165 + E190   Q169 + K269   R171 + G258   Q125 + K269   A225 + K269       T51 + G258   T165 + A225   Q169 + N270   R171 + N260   Q125 + W284   A225 + N270       T51 + N260   T165 + K242   Q169 + G273   R171 + K269   Q125 + Y295   A225 + G273       T51 + K269   T165 + S244   Q169 + S280   R171 + W284   Q125 + S304   A225 + S280       T51 + W284   T165 + G258   Q169 + W284   R171 + Y295   Q125 + V326   A225 + W284       T51 + Y295   T165 + N260   Q169 + T285   R171 + S304   G184 + A186   A225 + T285       T51 + S304   T165 + K269   Q169 + M286   R171 + V326   G184 + E190   A225 + M286       T51 + V326   T165 + W284   Q169 + F289   Q172 + A186   G184 + A225   A225 + F289       A186 + E190   T165 + Y295   Q169 + V291   Q172 + E190   G184 + K242   A225 + V291       A186 + T193   T165 + S304   Q169 + Y295   Q172 + A225   G184 + S244   A225 + Y295       A186 + N195   T165 + V326   Q169 + Q299   Q172 + K242   G184 + G258   A225 + Q299       A186 + A204   W167 + Q169   Q169 + S304   Q172 + S244   G184 + N260   A225 + S304       A186 + V206   W167 + A186   Q169 + R320   Q172 + G258   G184 + K269   A225 + R320       A186 + P211   W167 + E190   Q169 + H321   Q172 + N260   G184 + W284   A225 + H321       A186 + I214   W167 + A225   Q169 + S323   Q172 + K269   G184 + Y295   A225 + S323       A186 + V215   W167 + K242   Q169 + H324   Q172 + W284   G184 + S304   A225 + H324       A186 + L217   W167 + S244   Q169 + V326   Q172 + Y295   G184 + V326   A225 + V326       A186 + L219   W167 + G258   Q169 + F328   Q172 + S304   L235 + K242   A225 + F328       A186 + A225   W167 + N260   Q169 + T334   Q172 + V326   L235 + S244   A225 + T334       A186 + L235   W167 + K269   Q169 + D337   L173 + A186   L235 + G258   A225 + D337       A186 + V238   W167 + W284   Q169 + Q345   L173 + E190   L235 + N260   A225 + Q345       A186 + K242   W167 + Y295   Q169 + G346   L173 + A225   L235 + K269   A225 + G346       A186 + S244   W167 + S304   Q169 + G348   L173 + K242   L235 + W284   A225 + G348       A186 + M246   W167 + V326   Q169 + T355   L173 + S244   L235 + Y295   A225 + T355       A186 + M248   E190 + T193   Q169 + S376   L173 + G258   L235 + S304   A225 + S376       A186 + L250   E190 + N195   Q169 + D377   L173 + N260   L235 + V326   A225 + D377       A186 + G255   E190 + A204   Q169 + D379   L173 + K269   V238 + K242   A225 + D379       A186 + Q256   E190 + V206   Q169 + Y382   L173 + W284   V238 + S244   A225 + Y382       A186 + G258   E190 + P211   Q169 + S383   L173 + Y295   V238 + G258   A225 + S383       A186 + N260   E190 + I214   Q169 + Q385   L173 + S304   V238 + N260   A225 + Q385       A186 + A263   E190 + V215   Q169 + K391   L173 + V326   V238 + K269   A225 + K391       A186 + V264   E190 + L217   Q169 + K393   A174 + A186   V238 + W284   A225 + K393       A186 + Y267   E190 + L219   Q169 + Q395   A174 + E190   V238 + Y295   A225 + Q395       A186 + K269   E190 + A225   Q169 + A420   A174 + A225   V238 + S304   A225 + A420       A186 + N270   E190 + L235   Q169 + G423   A174 + K242   V238 + V326   A225 + G423       A186 + G273   E190 + V238   Q169 + T444   A174 + S244   K242 + S244   A225 + T444       A186 + S280   E190 + K242   Q169 + A445   A174 + G258   K242 + M246   A225 + A445       A186 + W284   E190 + S244   Q169 + Q449   A174 + N260   K242 + M248   A225 + Q449       A186 + T285   E190 + M246   Q169 + T459   A174 + K269   K242 + L250   A225 + T459       A186 + M286   E190 + M248   Q169 + P473   A174 + W284   K242 + G255   A225 + P473       A186 + F289   E190 + L250   Q169 + C474   A174 + Y295   K242 + Q256   A225 + C474       A186 + V291   E190 + G255   Q169 + G476   A174 + S304   K242 + G258   A225 + G476       A186 + Y295   E190 + Q256   Q169 + G477   A174 + V326   K242 + N260   A225 + G477       A186 + Q299   E190 + G258   Q169 + K484   L217 + A225   K242 + A263   A225 + K484       A186 + S304   E190 + N260   T193 + A225   L217 + K242   K242 + V264   S244 + M248       A186 + R320   E190 + A263   T193 + K242   L217 + S244   K242 + Y267   S244 + L250       A186 + H321   E190 + V264   T193 + S244   L217 + G258   K242 + K269   S244 + G255       A186 + S323   E190 + Y267   T193 + G258   L217 + N260   K242 + N270   S244 + Q256       A186 + H324   E190 + K269   T193 + N260   L217 + K269   K242 + G273   S244 + G258       A186 + V326   E190 + N270   T193 + K269   L217 + W284   K242 + S280   S244 + N260       A186 + F328   E190 + G273   T193 + W284   L217 + Y295   K242 + W284   S244 + A263       A186 + T334   E190 + S280   T193 + Y295   L217 + S304   K242 + T285   S244 + V264       A186 + D337   E190 + W284   T193 + S304   L217 + V326   K242 + M286   S244 + Y267       A186 + Q345   E190 + T285   T193 + V326   L219 + A225   K242 + F289   S244 + K269       A186 + G346   E190 + M286   N195 + A225   L219 + K242   K242 + V291   S244 + N270       A186 + G348   E190 + F289   N195 + K242   L219 + S244   K242 + Y295   S244 + G273       A186 + T355   E190 + V291   N195 + S244   L219 + G258   K242 + Q299   S244 + S280       A186 + S376   E190 + Y295   N195 + G258   L219 + N260   K242 + S304   S244 + W284       A186 + D377   E190 + Q299   N195 + N260   L219 + K269   K242 + R320   S244 + T285       A186 + D379   E190 + S304   N195 + K269   L219 + W284   K242 + H321   S244 + M286       A186 + Y382   E190 + R320   N195 + W284   L219 + Y295   K242 + S323   S244 + F289       A186 + S383   E190 + H321   N195 + Y295   L219 + S304   K242 + H324   S244 + V291       A186 + Q385   E190 + S323   N195 + S304   L219 + V326   K242 + V326   S244 + Y295       A186 + K391   E190 + H324   N195 + V326   M246 + G258   K242 + F328   S244 + Q299       A186 + K393   E190 + V326   A204 + A225   M246 + N260   K242 + T334   S244 + S304       A186 + Q395   E190 + F328   A204 + K242   M246 + K269   K242 + D337   S244 + R320       A186 + A420   E190 + T334   A204 + S244   M246 + W284   K242 + Q345   S244 + H321       A186 + G423   E190 + D337   A204 + G258   M246 + Y295   K242 + G346   S244 + S323       A186 + T444   E190 + Q345   A204 + N260   M246 + S304   K242 + G348   S244 + H324       A186 + A445   E190 + G346   A204 + K269   M246 + V326   K242 + T355   S244 + V326       A186 + Q449   E190 + G348   A204 + W284   M248 + G258   K242 + S376   S244 + F328       A186 + T459   E190 + T355   A204 + Y295   M248 + N260   K242 + D377   S244 + T334       A186 + P473   E190 + S376   A204 + S304   M248 + K269   K242 + D379   S244 + D337       A186 + C474   E190 + D377   A204 + V326   M248 + W284   K242 + Y382   S244 + Q345       A186 + G476   E190 + D379   V206 + A225   M248 + Y295   K242 + S383   S244 + G346       A186 + G477   E190 + Y382   V206 + K242   M248 + S304   K242 + Q385   S244 + G348       A186 + K484   E190 + S383   V206 + S244   M248 + V326   K242 + K391   S244 + T355       I214 + A225   E190 + Q385   V206 + G258   L250 + G258   K242 + K393   S244 + S376       I214 + K242   E190 + K391   V206 + N260   L250 + N260   K242 + Q395   S244 + D377       I214 + S244   E190 + K393   V206 + K269   L250 + K269   K242 + A420   S244 + D379       I214 + G258   E190 + Q395   V206 + W284   L250 + W284   K242 + G423   S244 + Y382       I214 + N260   E190 + A420   V206 + Y295   L250 + Y295   K242 + T444   S244 + S383       I214 + K269   E190 + G423   V206 + S304   L250 + S304   K242 + A445   S244 + Q385       I214 + W284   E190 + T444   V206 + V326   L250 + V326   K242 + Q449   S244 + K391       I214 + Y295   E190 + A445   P211 + A225   G255 + G258   K242 + T459   S244 + K393       I214 + S304   E190 + Q449   P211 + K242   G255 + N260   K242 + P473   S244 + Q395       I214 + V326   E190 + T459   P211 + S244   G255 + K269   K242 + C474   S244 + A420       V215 + A225   E190 + P473   P211 + G258   G255 + W284   K242 + G476   S244 + G423       V215 + K242   E190 + C474   P211 + N260   G255 + Y295   K242 + G477   S244 + T444       V215 + S244   E190 + G476   P211 + K269   G255 + S304   K242 + K484   S244 + A445       V215 + G258   E190 + G477   P211 + W284   G255 + V326   N260 + A263   S244 + Q449       V215 + N260   E190 + K484   P211 + Y295   Q256 + G258   N260 + V264   S244 + T459       V215 + K269   G258 + N260   P211 + S304   Q256 + N260   N260 + Y267   S244 + P473       V215 + W284   G258 + A263   P211 + V326   Q256 + K269   N260 + K269   S244 + C474       V215 + Y295   G258 + V264   A236 + K269   Q256 + W284   N260 + N270   S244 + G476       V215 + S304   G258 + Y267   A236 + W284   Q256 + Y295   N260 + G273   S244 + G477       V215 + V326   G258 + K269   A236 + Y295   Q256 + S304   N260 + S280   S244 + K484       N270 + W284   G258 + N270   A236 + S304   Q256 + V326   N260 + W284   K269 + N270       N270 + Y295   G258 + G273   A236 + V326   W284 + T285   N260 + T285   K269 + G273       N270 + S304   G258 + S280   V264 + K269   W284 + M286   N260 + M286   K269 + S280       N270 + V326   G258 + W284   V264 + W284   W284 + F289   N260 + F289   K269 + W284       G273 + W284   G258 + T285   V264 + Y295   W284 + V291   N260 + V291   K269 + T285       G273 + Y295   G258 + M286   V264 + S304   W284 + Y295   N260 + Y295   K269 + M286       G273 + S304   G258 + F289   V267 + V326   W284 + Q299   N260 + Q299   K269 + F289       G273 + V326   G258 + V291   Y267 + K269   W284 + S304   N260 + S304   K269 + V291       S280 + W284   G258 + Y295   Y267 + W284   W284 + R320   N260 + R320   K269 + Y295       S280 + Y295   G258 + Q299   Y267 + Y295   W284 + H321   N260 + H321   K269 + Q299       S280 + S304   G258 + S304   Y267 + S304   W284 + S323   N260 + S323   K269 + S304       S280 + V326   G258 + R320   Y267 + V326   W284 + H324   N260 + H324   K269 + R320       T285 + Y295   G258 + H321   A288 + F289   W284 + V326   N260 + V326   K269 + H321       T285 + S304   G258 + S323   A288 + V291   W284 + F328   N260 + F328   K269 + S323       T285 + V326   G258 + H324   A288 + Y295   W284 + T334   N260 + T334   K269 + H324       M286 + Y295   G258 + V326   A288 + Q299   W284 + D337   N260 + D337   K269 + V326       M286 + S304   G258 + F328   A288 + S304   W284 + Q345   N260 + Q345   K269 + F328       M286 + V326   G258 + T334   A288 + R320   W284 + G346   N260 + G346   K269 + T334       F289 + Y295   G258 + D337   A288 + H321   W284 + G348   N260 + G348   K269 + D337       F289 + S304   G258 + Q345   A288 + S323   W284 + T355   N260 + T355   K269 + Q345       F289 + V326   G258 + G346   A288 + H324   W284 + S376   N260 + S376   K269 + G346       V291 + Y295   G258 + G348   A288 + V326   W284 + D377   N260 + D377   K269 + G348       V291 + S304   G258 + T355   A288 + F328   W284 + D379   N260 + D379   K269 + T355       V291 + V326   G258 + S376   A288 + T334   W284 + Y382   N260 + Y382   K269 + S376       Y295 + Q299   G258 + D377   A288 + D337   W284 + S383   N260 + S383   K269 + D377       Y295 + S304   G258 + D379   A288 + Q345   W284 + Q385   N260 + Q385   K269 + D379       Y295 + R320   G258 + Y382   A288 + G346   W284 + K391   N260 + K391   K269 + Y382       Y295 + H321   G258 + S383   A288 + G348   W284 + K393   N260 + K393   K269 + S383       Y295 + S323   G258 + Q385   A288 + T355   W284 + Q395   N260 + Q395   K269 + Q385       Y295 + H324   G258 + K391   A288 + S376   W284 + A420   N260 + A420   K269 + K391       Y295 + V326   G258 + K393   A288 + D377   W284 + G423   N260 + G423   K269 + K393       Y295 + F328   G258 + Q395   A288 + D379   W284 + T444   N260 + T444   K269 + Q395       Y295 + T334   G258 + A420   A288 + Y382   W284 + A445   N260 + A445   K269 + A420       Y295 + D337   G258 + G423   A288 + S383   W284 + Q449   N260 + Q449   K269 + G423       Y295 + Q345   G258 + T444   A288 + Q385   W284 + T459   N260 + T459   K269 + T444       Y295 + G346   G258 + A445   A288 + K391   W284 + P473   N260 + P473   K269 + A445       Y295 + G348   G258 + Q449   A288 + K393   W284 + C474   N260 + C474   K269 + Q449       Y295 + T355   G258 + T459   A288 + Q395   W284 + G476   N260 + G476   K269 + T459       Y295 + S376   G258 + P473   A288 + A420   W284 + G477   N260 + G477   K269 + P473       Y295 + D377   G258 + C474   A288 + G423   W284 + K484   N260 + K484   K269 + C474       Y295 + D379   G258 + G476   A288 + T444   S304 + V326   S304 + K393   K269 + G476       Y295 + Y382   G258 + G477   A288 + A445   S304 + Y295   S304 + Q395   K269 + G477       Y295 + S383   G258 + K484   A288 + Q449   S304 + S304   S304 + A420   K269 + K484       Y295 + Q385   Q299 + S304   A288 + T459   S304 + V326   S304 + G423   S304 + T355       Y295 + K391   Q299 + V326   A288 + P473   S304 + Q299   S304 + T444   S304 + S376       Y295 + K393   R320 + V326   A288 + C474   S304 + S304   S304 + A445   S304 + D377       Y295 + Q395   H321 + V326   A288 + G476   S304 + R320   S304 + Q449   S304 + D379       Y295 + A420   S323 + V326   A288 + G477   S304 + H321   S304 + T459   S304 + Y382       Y295 + G423   H324 + V326   A288 + K484   S304 + S323   S304 + P473   S304 + S383       Y295 + T444   Y295 + P473   S304 + Q345   S304 + H324   S304 + C474   S304 + Q385       Y295 + A445   Y295 + C474   S304 + G346   S304 + V326   S304 + G476   S304 + K391       Y295 + Q449   Y295 + G476   S304 + G348   S304 + F328   S304 + G477   S304 + D337       Y295 + T459   Y295 + G477   Y295 + K484   S304 + T334   S304 + K484                    
wherein numbering is according to SEQ ID NO; 13; or
 
                                                    H1 + A37   N54 + F113   K72 + G109   R116 + A174   T165 + A174   N195 + V206       H1 + T40   N54 + R116   K72 + F113   R116 + G184   T165 + G184   N195 + L228       H1 + N54   N54 + N125   K72 + R116   R116 + N195   T165 + N195   N195 + G255       H1 + V56   N54 + F133   K72 + N125   R116 + G196   T165 + G196   N195 + A265       H1 + A60   N54 + T134   K72 + F133   R116 + A204   T165 + A204   N195 + S280       H1 + K72   N54 + T165   K72 + T134   R116 + V206   T165 + V206   N195 + S304       H1 + G109   N54 + Q172   K72 + T165   R116 + L228   T165 + L228   N195 + R320       H1 + F113   N54 + L173   K72 + Q172   R116 + G255   T165 + G255   N195 + H321       H1 + R116   N54 + A174   K72 + L173   R116 + A265   T165 + A265   N195 + S323       H1 + N125   N54 + G184   K72 + A174   R116 + S280   T165 + S280   N195 + K391       H1 + F133   N54 + N195   K72 + G184   R116 + S304   T165 + S304   G196 + A204       H1 + T134   N54 + G196   K72 + N195   R116 + R320   T165 + R320   G196 + V206       H1 + T165   N54 + A204   K72 + G196   R116 + H321   T165 + H321   G196 + L228       H1 + Q172   N54 + V206   K72 + A204   R116 + S323   T165 + S323   G196 + G255       H1 + L173   N54 + L228   K72 + V206   R116 + K391   T165 + K391   G196 + A265       H1 + A174   N54 + G255   K72 + L228   N125 + F133   Q172 + L173   G196 + S280       H1 + G184   N54 + A265   K72 + G255   N125 + T134   Q172 + A174   G196 + S304       H1 + N195   N54 + S280   K72 + A265   N125 + T165   Q172 + G184   G196 + R320       H1 + G196   N54 + S304   K72 + S280   N125 + Q172   Q172 + N195   G196 + H321       H1 + A204   N54 + R320   K72 + S304   N125 + L173   Q172 + G196   G196 + S323       H1 + V206   N54 + H321   K72 + R320   N125 + A174   Q172 + A204   G196 + K391       H1 + L228   N54 + S323   K72 + H321   N125 + G184   Q172 + V206   A204 + V206       H1 + G255   N54 + K391   K72 + S323   N125 + N195   Q172 + L228   A204 + L228       H1 + A265   V56 + A60   K72 + K391   N125 + G196   Q172 + G255   A204 + G255       H1 + S280   V56 + K72   G109 + F113   N125 + A204   Q172 + A265   A204 + A265       H1 + S304   V56 + G109   G109 + R116   N125 + V206   Q172 + S280   A204 + S280       H1 + R320   V56 + F113   G109 + N125   N125 + L228   Q172 + S304   A204 + S304       H1 + H321   V56 + R116   G109 + F133   N125 + G255   Q172 + R320   A204 + R320       H1 + S323   V56 + N125   G109 + T134   N125 + A265   Q172 + H321   A204 + H321       H1 + K391   V56 + F133   G109 + T165   N125 + S280   Q172 + S323   A204 + S323       A37 + T40   V56 + T134   G109 + Q172   N125 + S304   Q172 + K391   A204 + K391       A37 + N54   V56 + T165   G109 + L173   N125 + R320   L173 + A174   V206 + L228       A37 + V56   V56 + Q172   G109 + A174   N125 + H321   L173 + G184   V206 + G255       A37 + A60   V56 + L173   G109 + G184   N125 + S323   L173 + N195   V206 + A265       A37 + K72   V56 + A174   G109 + N195   N125 + K391   L173 + G196   V206 + S280       A37 + G109   V56 + G184   G109 + G196   F133 + T134   L173 + A204   V206 + S304       A37 + F113   V56 + N195   G109 + A204   F133 + T165   L173 + V206   V206 + R320       A37 + R116   V56 + G196   G109 + V206   F133 + Q172   L173 + L228   V206 + H321       A37 + N125   V56 + A204   G109 + L228   F133 + L173   L173 + G255   V206 + S323       A37 + F133   V56 + V206   G109 + G255   F133 + A174   L173 + A265   V206 + K391       A37 + T134   V56 + L228   G109 + A265   F133 + G184   L173 + S280   L228 + G255       A37 + T165   V56 + G255   G109 + S280   F133 + N195   L173 + S304   L228 + A265       A37 + Q172   V56 + A265   G109 + S304   F133 + G196   L173 + R320   L228 + S280       A37 + L173   V56 + S280   G109 + R320   F133 + A204   L173 + H321   L228 + S304       A37 + A174   V56 + S304   G109 + H321   F133 + V206   L173 + S323   L228 + R320       A37 + G184   V56 + R320   G109 + S323   F133 + L228   L173 + K391   L228 + H321       A37 + N195   V56 + H321   G109 + K391   F133 + G255   A174 + G184   L228 + S323       A37 + G196   V56 + S323   F113 + R116   F133 + A265   A174 + N195   L228 + K391       A37 + A204   V56 + K391   F113 + N125   F133 + S280   A174 + G196   G255 + A265       A37 + V206   A60 + K72   F113 + F133   F133 + S304   A174 + A204   G255 + S280       A37 + L228   A60 + G109   F113 + T134   F133 + R320   A174 + V206   G255 + S304       A37 + G255   A60 + F113   F113 + T165   F133 + H321   A174 + L228   G255 + R320       A37 + A265   A60 + R116   F113 + Q172   F133 + S323   A174 + G255   G255 + H321       A37 + S280   A60 + N125   F113 + L173   F133 + K391   A174 + A265   G255 + S323       A37 + S304   A60 + F133   F113 + A174   T134 + T165   A174 + S280   G255 + K391       A37 + R320   A60 + T134   F113 + G184   T134 + Q172   A174 + S304   A265 + S280       A37 + H321   A60 + T165   F113 + N195   T134 + L173   A174 + R320   A265 + S304       A37 + S323   A60 + Q172   F113 + G196   T134 + A174   A174 + H321   A265 + R320       A37 + K391   A60 + L173   F113 + A204   T134 + G184   A174 + S323   A265 + H321       T40 + N54   A60 + A174   F113 + V206   T134 + N195   A174 + K391   A265 + S323       T40 + V56   A60 + G184   F113 + L228   T134 + G196   G184 + N195   A265 + K391       T40 + A60   A60 + N195   F113 + G255   T134 + A204   G184 + G196   S280 + S304       T40 + K72   A60 + G196   F113 + A265   T134 + V206   G184 + A204   S280 + R320       T40 + G109   A60 + A204   F113 + S280   T134 + L228   G184 + V206   S280 + H321       T40 + F113   A60 + V206   F113 + S304   T134 + G255   G184 + L228   S280 + S323       T40 + R116   A60 + L228   F113 + R320   T134 + A265   G184 + G255   S280 + K391       T40 + N125   A60 + G255   F113 + H321   T134 + S280   G184 + A265   S304 + R320       T40 + F133   A60 + A265   F113 + S323   T134 + S304   G184 + S280   S304 + H321       T40 + T134   A60 + S280   F113 + K391   T134 + R320   G184 + S304   S304 + S323       T40 + T165   A60 + S304   R116 + N125   T134 + H321   G184 + R320   S304 + K391       T40 + Q172   A60 + R320   R116 + F133   T134 + S323   G184 + H321   R320 + H321       T40 + L173   A60 + H321   R116 + T134   T134 + K391   G184 + S323   R320 + S323       T40 + A174   A60 + S323   R116 + T165   T165 + Q172   G184 + K391   R320 + K391       T40 + G184   A60 + K391   R116 + Q172   T165 + L173   N195 + G196   H321 + S323       T40 + N195   T40 + S323   R116 + L173   H1 + Q169   N195 + A204   H321 + K391       T40 + G196   T40 + K391   T40 + A265   H1 + A186   S244 + G255   S323 + K391       T40 + A204   N54 + V56   T40 + S280   H1 + E190   S244 + G258   A265 + K269       T40 + V206   N54 + A60   T40 + S304   H1 + A225   S244 + N260   A265 + W284       T40 + L228   N54 + K72   T40 + R320   H1 + K242   S244 + A265   A265 + Y295       T40 + G255   N54 + G109   T40 + H321   H1 + S244   S244 + K269   A265 + S304       T40 + Q169   R116 + Q169   A204 + A225   H1 + G258   S244 + S280   A265 + V326       T40 + A186   R116 + A186   A204 + K242   H1 + N260   S244 + W284   K269 + S280       T40 + E190   R116 + E190   A204 + S244   H1 + K269   S244 + Y295   K269 + W284       T40 + A225   R116 + A225   A204 + G258   H1 + W284   S244 + S304   K269 + Y295       T40 + K242   R116 + K242   A204 + N260   H1 + Y295   S244 + R320   K269 + S304       T40 + S244   R116 + S244   A204 + K269   H1 + S304   S244 + H321   K269 + R320       T40 + G258   R116 + G258   A204 + W284   H1 + V326   S244 + S323   K269 + H321       T40 + N260   R116 + N260   A204 + Y295   A37 + Q169   S244 + V326   K269 + S323       T40 + K269   R116 + K269   A204 + S304   A37 + A186   S244 + K391   K269 + V326       T40 + W284   R116 + W284   V206 + A225   A37 + E190   S244 + I405   K269 + K391       T40 + Y295   R116 + Y295   V206 + K242   A37 + A225   S244 + A421   K269 + I405       T40 + S304   R116 + S304   V206 + S244   A37 + K242   S244 + A422   K269 + A421       T40 + V326   N125 + Q169   V206 + G258   A37 + S244   S244 + A428   K269 + A422       N54 + Q169   N125 + A186   V206 + N260   A37 + G258   S244 + G448   K269 + A428       N54 + A186   N125 + E190   V206 + K269   A37 + N260   S244 + D476   K269 + G448       N54 + E190   N125 + A225   V206 + W284   A37 + K269   S244 + G477   K269 + D476       N54 + A225   N125 + K242   V206 + Y295   A37 + W284   G255 + G258   K269 + G477       N54 + K242   N125 + S244   V206 + S304   A37 + Y295   G255 + N260   S280 + W284       N54 + S244   N125 + G258   V206 + V326   A37 + S304   G255 + K269   S280 + Y295       N54 + G258   N125 + N260   A225 + L228   A37 + V326   G255 + W284   S280 + S304       N54 + N260   N125 + K269   A225 + K242   Q172 + A186   G255 + Y295   S280 + V326       N54 + K269   N125 + W284   A225 + S244   Q172 + E190   G255 + S304   W284 + Y295       N54 + W284   N125 + Y295   A225 + G255   Q172 + A225   G255 + V326   W284 + S304       N54 + Y295   N125 + S304   A225 + G258   Q172 + K242   G258 + N260   W284 + R320       N54 + S304   F133 + Q169   A225 + N260   Q172 + S244   G258 + A265   W284 + H321       N54 + V326   F133 + A186   A225 + A265   Q172 + G258   G258 + K269   W284 + S323       V56 + Q169   F133 + E190   A225 + K269   Q172 + N260   G258 + S280   W284 + V326       V56 + A186   F133 + A225   A225 + S280   Q172 + K269   G258 + W284   W284 + K391       V56 + E190   F133 + K242   A225 + W284   Q172 + W284   G258 + Y295   W284 + I405       V56 + A225   F133 + S244   A225 + Y295   Q172 + Y295   G258 + S304   W284 + A421       V56 + K242   F133 + G258   A225 + S304   Q172 + S304   G258 + R320   W284 + A422       V56 + S244   F133 + N260   A225 + R320   L173 + A186   G258 + H321   W284 + A428       V56 + G258   F133 + K269   A225 + H321   L173 + E190   G258 + S323   W284 + G448       V56 + N260   F133 + W284   A225 + S323   L173 + A225   G258 + V326   W284 + D476       V56 + K269   F133 + Y295   A225 + V326   L173 + K242   G258 + K391   W284 + G477       V56 + W284   F133 + S304   A225 + K391   L173 + S244   G258 + I405   Y295 + S304       V56 + Y295   T134 + Q169   A225 + I405   L173 + G258   G258 + A421   Y295 + R320       V56 + S304   T134 + A186   A225 + A421   L173 + N260   G258 + A422   Y295 + H321       V56 + V326   T134 + E190   A225 + A422   L173 + K269   G258 + A428   Y295 + S323       A60 + Q169   T134 + A225   A225 + A428   L173 + W284   G258 + G448   Y295 + V326       A60 + A186   T134 + K242   A225 + G448   L173 + Y295   G258 + D476   Y295 + K391       A60 + E190   T134 + S244   A225 + D476   L173 + S304   G258 + G477   Y295 + I405       A60 + A225   T134 + G258   A225 + G477   A174 + A186   N260 + A265   Y295 + A421       A60 + K242   T134 + N260   L228 + K242   A174 + E190   N260 + K269   Y295 + A422       A60 + S244   T134 + K269   L228 + S244   A174 + A225   N260 + S280   Y295 + A428       A60 + G258   T134 + W284   L228 + G258   A174 + K242   N260 + W284   Y295 + G448       A60 + N260   T134 + Y295   L228 + N260   A174 + S244   N260 + Y295   Y295 + D476       A60 + K269   T134 + S304   L228 + K269   A174 + G258   N260 + S304   Y295 + G477       A60 + W284   T134 + V326   L228 + W284   A174 + N260   N260 + R320   S304 + R320       A60 + Y295   T165 + Q169   L228 + Y295   A174 + K269   N260 + H321   S304 + H321       A60 + S304   T165 + A186   L228 + S304   A174 + W284   N260 + S323   S304 + S323       A60 + V326   T165 + E190   L228 + V326   A174 + Y295   N260 + V326   S304 + V326       K72 + Q169   T165 + N195   K242 + S244   A174 + S304   N260 + K391   S304 + K391       K72 + A186   T165 + A225   K242 + G255   G184 + A186   N260 + I405   S304 + I405       K72 + E190   T165 + K242   K242 + G258   G184 + E190   N260 + A421   S304 + A421       K72 + A225   T165 + S244   K242 + N260   G184 + A225   N260 + A422   S304 + A422       K72 + K242   T165 + G258   K242 + A265   G184 + K242   N260 + A428   S304 + A428       K72 + S244   T165 + N260   K242 + K269   G184 + S244   N260 + G448   S304 + G448       K72 + G258   T165 + K269   K242 + S280   G184 + G258   N260 + D476   S304 + D476       K72 + N260   T165 + W284   K242 + W284   G184 + N260   N260 + G477   S304 + G477       K72 + K269   T165 + Y295   K242 + Y295   G184 + K269   N195 + A225   R320 + H321       K72 + W284   T165 + S304   K242 + S304   G184 + W284   N195 + K242   R320 + V326       K72 + Y295   Q169 + Q172   K242 + R320   G184 + Y295   N195 + S244   H321 + V326       K72 + S304   Q169 + L173   K242 + H321   G184 + S304   N195 + G258   S323 + V326       G109 + Q169   Q169 + A174   K242 + S323   A186 + E190   N195 + N260   V326 + K391       G109 + A186   Q169 + G184   K242 + V326   A186 + N195   N195 + K269   V326 + I405       G109 + E190   Q169 + A186   K242 + K391   A186 + G196   N195 + W284   V326 + A421       G109 + A225   Q169 + E190   K242 + I405   A186 + A204   N195 + Y295   V326 + A422       G109 + K242   Q169 + N195   K242 + A421   A186 + V206   N195 + S304   V326 + A428       G109 + S244   Q169 + G196   K242 + A422   A186 + A225   N195 + V326   V326 + G448       G109 + G258   Q169 + A204   K242 + A428   A186 + L228   G196 + A225   V326 + D476       G109 + N260   Q169 + V206   K242 + G448   A186 + K242   G196 + K242   V326 + G477       G109 + K269   Q169 + A225   K242 + D476   A186 + S244   G196 + S244   E190 + Y295       G109 + W284   Q169 + L228   K242 + G477   A186 + G255   G196 + G258   E190 + S304       G109 + Y295   Q169 + K242   E190 + A204   A186 + G258   G196 + N260   E190 + R320       G109 + S304   Q169 + S244   E190 + V206   A186 + N260   G196 + K269   E190 + H321       G109 + V326   Q169 + G255   E190 + A225   A186 + A265   G196 + W284   E190 + S323       F113 + Q169   Q169 + G258   E190 + L228   A186 + K269   G196 + Y295   E190 + V326       F113 + A186   Q169 + N260   E190 + K242   A186 + S280   G196 + S304   E190 + K391       F113 + E190   Q169 + A265   E190 + S244   A186 + W284   G196 + V326   E190 + I405       F113 + A225   Q169 + K269   E190 + G255   A186 + Y295   A186 + G448   E190 + A421       F113 + K242   Q169 + S280   E190 + G258   A186 + S304   A186 + D476   E190 + A422       F113 + S244   Q169 + W284   E190 + N260   A186 + R320   A186 + G477   E190 + A428       F113 + G258   Q169 + Y295   E190 + A265   A186 + H321   E190 + N195   E190 + G448       F113 + N260   Q169 + S304   E190 + K269   A186 + S323   E190 + G196   E190 + D476       F113 + K269   Q169 + R320   E190 + S280   A186 + V326   Q169 + A422   E190 + G477       F113 + W284   Q169 + H321   E190 + W284   A186 + K391   Q169 + A428   A186 + A422       F113 + Y295   Q169 + S323   Q169 + I405   A186 + I405   Q169 + G448   A186 + A428       F113 + S304   Q169 + V326   Q169 + A421   A186 + A421   Q169 + D476       F113 + V326   Q169 + K391           Q169 + G477                    
wherein numbering is according to SEQ ID NO: 14.
 
     Stability of a parent polypeptide may be improved by introducing a pairwise deletion within the B domain of parent polypeptide. Thus, in one embodiment, the said variant comprises
         a) a deletion and/or a substitution at two or more positions corresponding to positions R181, G182, D183, and G184 of the amino acid sequence as set forth in SEQ ID NOs: 7 or 10, and   b) an alteration at one or more positions corresponding to positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50. T51, N54, V56, K72. R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328. T334, D337, Q345, G346, G348, T355, S376. D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q172, L173, A174, G184, N195, G196, A204, V206, L228, G255, A265, S280, S304, R320, H321, S323, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14.       

     The term “pairwise deletion” as used herein, refers to a deletion of an amino acid in two positions. Said two positions may be adjacent to one another but may also be separated by one, two, three, four, or five amino acids. 
     In a particular embodiment, the deletion a) is selected from the group consting of R18I+G182, R18I+D183, R18I+G184, G182+D183, G182+G184, or D18S+G184. 
     In an embodiment, variant comprises one or more of the following alterations; H1*, T5K, G7K, G7A, Q11H, N16S, N16H, V17L, Q32S, A37H, A37M, A37V, A37S, A37Y, A37R, A37L, T40G, T40K, P45A, W48G, W48Y, W48F, G50A, T51K, T51E, T51G, T51A, T51S, T51G, T51D. N54S, V56T, K72R, K72H, K72S, K72Q, K72E, K72N, K72A, K72M, R87S, Q98S, Q98A, M105F, M105I, M105V, M105L, G109A, G109S, F113W, F113S, F113N, F113Y, F113R, F113L, F113Q, R116Q, R116V, R116K, R116W, R116L, R116A, R116H, R116M, R116E, R116S, R116I, R116G, Q118N, Q118K, Q118G, Q118S, Q118F, Q118R, Q125P, Q125K, Q125A, Q125T, G133S, T134E, W140Y, G142T, G149Q, T165S, T165G, T165V, W167I, W167G, W167F, W167R, W167S, W167H, W167L, W167M, W167Y, Q169E, R171H, Q172G, Q172R, Q172N, Q172D, Q172Y, Q172M, Q172S, Q172T, Q172K, Q172H, Q172E, L173V, L173G, L173H, L173A, L173I, L173P, L173I, L173F, L173M, A174S, A174D, A174P, A174M, A174T, A174H, A174K, A174G, A174Q, A174N, A174V, A174L, G184T, A186D, A186N, A186E, A186Q, A186H, E190P, T193K, N195F, A204T, A204V, A204G, A204G, V206L, V206S, V206Y, P211D, I214G, I214H. I214S, I214T, I214L, I214E, I214W, V215T, L217T, L217Q, L219V, L219H, A225V, L235V, L235A, V238A, V238I, V238G, K242Q, S244Q, M246L, M246A, M246I, M246F, M246V, M246S, M248T, L250I, L250V, L250I, L250A, L250F, L250M, G255N, G255A, G255S, Q256A, N260G, A263G, V264I, V264T, Y267I, Y267M, Y267H, Y267L, K269Q, N270G, N270T, G273R, S280W, S280L, S280T, S280A, S280K, S280Q, W284H, T285L, T285Q, M286F, M286L, A288L, A288V, F289I, F289L. V291G, V291A, V291T, Y295F, Y295N. Q299V. S304R, S304N, R320A, R320V, H321Y, S323N, H324L, V326L, F328L, F328M, F328V, F328I, T334S, D337H, Q345D, G346T, G346D, G346P, G348S, G348P, T355L, T355F, S376H, S376T, S376V, D377H, D377S, D377A, D377Q, D379S, D379G, D379R, D379A, Y382M, Y382I, Y382L, Y382F, S383G, S383A, Q385L, K391A, K391Y, K391V, K391M, K391E, K391D, K391R, K391H, K391W, K391I, K391Q, K391L, K393Y, K393R, K393Q, K393S, Q395P, T400P, H402R, A420Q, A420S, A420K, A420L, G423H, T444Q, T444S, T444D, T444Y, T444H, T444V, T444R, T444A, A445Q, Q449T, T459N, P473R, P473A, P473G, P473T, P473K, Q474V, G476K, G477A, G477Q, K484A, K484G, K484P, K484E, K484Q, and K484S of SEQ ID NO: 13; or H1*, G7A, A37H, T40D, W148Y, W48F, N54S, V56T, A60V, K72R, Q98A, G109A, F113Q, R116H, R116V, R116Q, N125D, F133Q, T134E, T165G, Q169E, Q172D, Q172G, Q172N, L173V, A174S, G184T, A186D, A186N, A186E, A186Q, A186H, E190P, N195F, G196R, A204G, V206L, V206Y, A225V, L228I, K242Q, S244Q, G255A, G255S, N260G, A265G, K269Q, N270T, S280Q, W284H, A288L, A288V, F289L, Y295F, Y295N, S304N, S304R, S304N, R320A, H321Y, S323N, V326L, K391A, I405L, A421H, A422P, A428T, G448D, D476K, D476G, D476N, D476Y, G477S, G477A, G477T, and G477Q of SEQ ID NO: 14. 
     In a particular embodiment, the variant has an IF of &gt;1 when compared to said parent polypeptide, and wherein said variant is selected from
     G182*+D183*+N195F   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116W+A174S+G182A+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1A+N54S+V56T+G109A+G142T+A174S+G182A+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+V213I+K391A+G476K   H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q118K+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1A+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+D377R+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K   H1*+N54S+V56T+G109A+W167I+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1A+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+G346P+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L-H214H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+I214T+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391A+P473R+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280I+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255N+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285Q+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q449T+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473K+G476K   H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K   H1*+G50A+N54S+V5 G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484E   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484Q   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S   H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K   H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+H324L+K391A+G476K   H1*+N54S+V56T+G109A+G149Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A   H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q   H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G423H+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y   H1*+T5K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K   H1*+G50A+N54S+V56I+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K   H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K   H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280K+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A   H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G337H+Q385L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+M105F+G109A+Q118F+T143E+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K   H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K   H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355F+K391A+G476K   H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K   H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K   H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K   H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51A+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51 S+N54S+V56T+G109A+A174P+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51G+N54S+V56T+G109A+A174M+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72A+G109A+W167S+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+Q11H+N54S+V56T+G109A+Q118R+T134E+A174S+G184T+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184I+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A   H1*+N54S+V56T+K72A+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+W167S+G182*+D183*+N195F+A174S+V206L+K391A+G476K   H1*+N54S+V56T+K72H+G109A+W167S+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T K72R+G109A+W167T+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72E+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+W167M+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184I+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K   H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184A+T193K+N195F+V206L+K391A+G476K   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183A+N195F+V206L+R320A+S323N+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291G+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328M+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+F328L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328V+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328I+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291T+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q125T+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N16S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72A+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184I+N195F+V206L+R320A+S323N+K391A+G476K   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217I+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K   H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217Q+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346D+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D*+G184T+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K   H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K   H1*+N54S+V56T+G109A+R116I+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174F+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K   H1*+T51G+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51G+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182+*D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51D+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+T51A+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T K72R+G109A+Q172R+A174S+K179E+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K   H1*+N54S+V56T+K72R+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N5F+V206L+K391A+G476K   H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391S+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K   H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L S323N+K391A+G476K   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225F+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+M246A+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382I+K391A+G476K   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D376H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K   H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+A204T+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+T165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+M286F+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267I+D377H+K39A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K   H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+M105L+G109A+A174S+G182A+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246I+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250I+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391V+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K   H1*+N54R+V56T+G109A+A174S+G182*+D183K+N195F+V206L+K391A+G476K   H1*+N54L+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109S+A174S+G182*+D183A+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174R+G182*+D183K+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206S+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+K391H+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391 W+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391I+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+L250F+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Q+G476K   H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172E+L173A+A174R+G182*+D183A+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72Q+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K   H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376I+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K   H1*+N54S+V56T+G109A+Q172E+L173I+A174N+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L-FV238T+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+K391A+G476K   H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K   H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172K+L173V+A174T+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172E+I173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172E+L173T+A174R+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+K391A+G476K   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286F+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267I+K391A+G476K   H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*4N195F+A204G+V206L+G255A+K391A+P473R+G476K   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184I+N195F+A204G+V206L+K391A+P473R+G476K   H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K   H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A   H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G2*+D1*+N195F+V206L+K391A+G346T+G477A+G476K   H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K   H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D*+N195F+V206L+V264I+K391A+C474V+G476K   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182 D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F289I+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+R6H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+Q172N+A174S+G182W+D183*+N195F+V206L+K391A+G476K   H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+R6H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A74S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37V+N54S+V56I+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K   H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K   H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+F113Q+67F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K   H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37V+N54S+V56T+A60V+G109A+R6Q+I165G+A174S+G182*+D183*+N195F+V206+K391A+G476K   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206+K391A+G476K   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K   H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L.   N54S+V56I+G109A+G182*+D183*+N195E   N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L   H1*W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K   N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A   H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K   H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K   H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37V+N54S+V56I+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K   H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+G476K   G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391T+G476K   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K   H1*+N54S+V56T+G109A+F113Q+67F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K   H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K   H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37H+N54S+V56T+G109A+R6H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K   H1*+G109A+G182*+D183*+N195F+V206L+K391A+G476K   G109A+G182*+D183K+N195F+V206L+G476K   H1*+G109A+G182*+D183*+N195F+K391A   H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H   H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K   H1A+N54S+V56T+G109A+P124A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K   H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+V264F+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K   H1A+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K   H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183A+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1A+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K   H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K   H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184I+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K,   H1A+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174K+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K,   H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K.   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   G182*+D183*+N195F,   H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K,   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182+D183*+G184I+N195F+V206L+K391A+P473R,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174K+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183K+N195F+V206L+Y295F+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K,   F113Q+R116H+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   Q169E+Q172K+A174*+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   F113Q+R116Q+D183*+G182*+N195F,   N54S+V56T+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   Q169E+Q172K+G182*+D183*+N195F,   G182*+D183*+A186D,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K,   G182*+D183*+A186N,   H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K,   H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S244Q+K391A+G476K,   G182*+D183*+F328L,   G182*+D183*+N195F+A288V,   G182*+D183*+A186N+N195F,   G182*+D183*+E190P+N195F,   W140Y+G182*+D183*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225V+K391A+G476K,   G182*+D183*+A186D+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+W48Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F+V238A,   G182*+D183*+Y295N,   Q172G+G182*+D183*,   F113Q+G182*+D183*+N195F,   H1*+N54S+V56T+Q98A+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F+Y295F,   G182*+D183*+A186E,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206Y+K391A+G476K,   G182*+D183*+N195F+\A/284H,   G182*+D183*+A186Q+N195F,   K72R+G182*+D183*,   D183*+G182*+N195F+V326L,   D183*+G182*+A186E+N195F,   Q169E+G182*+D183*+N195F,   D183*+G182*+N195F+K242Q,   G7A+G182*+D183*+N195F,   D183*+G182*+N195F+N260G,   D183*+G182*+R321A+S323N,   D183*+G182*+W284H,   H1*+D183*+G182*,   D183*+G182*+A186H+N195F,   D183*+G182*+F289V,   D183*+G182*+K391A,   D183*+G182*+S304R,   W167F+G182*+D183*+N195F,   F113Q+R116H+D183*+G182*,   D183*+G182*+N270G,   D183*+G182*+S244Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   D183*+G182*+N195F+Y295N,   W48F+D183*+G182*,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   D183*+G182*I-A186Q,   W140Y+D183*+G182*,   D183*+G182*+K242Q,   D183*+G182*+N206L,   D183*+G182*+N195F+V206F,   Q169E+D183*+G182*,   D183*+G182*+N195F+R320A,   G182*I-D183*+G184T+N195F,   D183*+G182*+G184T,   N54S+V56T+D183*1-G182*,   D183*+G182*+P473R,   W48Y+D183*+G182*,   D183*+G182*+R323A,   G7A+D183*+G182*,   G109A+D183+G182*,   W167F+D183*+G182*,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   A174S+D183*4-G182*,   R116Q+D183*+G182*,   D183*+G182*+N195+V291A,   Q172K+D183*+G182*,   N54S+D183*+G182*,   D183*+G182*+N195F+S323N,   D183*+G182*+N195F+M246V,   W48Y+D183*+G182*+N195F,   D183*+G182*+N195F+S304R,
       V56T+D183*+G182*, wherein numbering is according to SEQ ID NO; 13; and   
       N54S+G182*+D183*+N195F,   G109A+G182*+D183*+N195F,   G182*+D183*+N195F+K391A,   G182*+D183*+N195F+G476K,   H1*+G182*+D183*+N195F,   N54S+G182*+D183*+N195F,   V56T+G182*+D183*A-N195F,   G182*+D183*+N195F+I405L,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+D476K,   A174S+G182*+D183*+N195F,   H1*+N54S+V56I+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477S,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S304N+I405L+A421H+A422P+A428T+D476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G+G477T,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477Q.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476N,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476Y,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206I+I405L+A421H+A422P+A428I   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+G196R+V206L+G255A+I405L+A421H+A422P+A428T+N475S,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116Q+G133Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+G448D,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+I173V+A174S+G182*+D1*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+F116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+M105F+G182*+D183*+N195F+V206L+L228I+R320A+S323N+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO:14.   

     As can be seen from the data obtained in Examples 3 and 4, all tested variants have an Improvement Factor (IF) of at least 1.0, i.e. at least on par with a parent polypeptide having SEQ ID NO: 15, when tested in either (i) a detergent Model A at 15° C. and an enzyme concentration of 0.05 mg/L, (ii) a detergent Model A at 15° C. and an enzyme concentration of 0.2 mg/L, (iii) a detergent Model J at 15° C. and an enzyme concentration of 0.05 mg/L, or (iv) a detergent Model J at 15° C. and an enzyme concentration of 0.2 mg/L. Thus, the variants of the present invention have even in different model detergent compositions, at a low temperature as well as in a low concentrations, a wash performance at least that of the parent polypeptide having SEQ ID NO: 15. Specifications of the model detergents and specific test conditions for the AMSA, can be seen in Examples 3 and 4. 
     In another embodiment, the variant is selected from the group consisting of;
     G182*+D183*+N195F+V206F+N260P+L312I+L313I+R320A+T355L+L389I+T400P,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G50A+T51A+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+T40K+N54S+V56T+G109A+G149H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+Q22N+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+H28N+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N29S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+H28N+N29S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+R176K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+R176K+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+N175G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+Y295N+Q299T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269S+A274K+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235I+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+V238A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q365S+K391A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+H402R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320S+H321N+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125S+A174S+G182*+D183A+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+G476K,   H1*+T51Q+N54S+V56T+G109A+A174S+G182*+D183*4N195F+Y203F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N175Q+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+H28N+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+M202V+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+Y203G+V206L+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+G110N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125S+A174S+N175G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+V291A+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+A265G+V291A+F328L+Q365S+K391A+G476K,   H1*+N16Y+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+G337E+D377H+K391A+G476K,   H1*+I9L+N54S+V56T+G109A+Q125S+V131T+A174*+G182*+D183*+V206L+Y267F+F289H+Q361E+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182 D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184I+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+C) 172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K,   H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F,   H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K,   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184I+N195F+V206L+K391A+P473R,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K,   F113Q+R116H+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   Q169E+Q172K+A174*+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K269Q+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   F113Q+R116Q+D183*+G182*+N195F,   N54S+V56T+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   Q169E+Q172K+G182*+D183*+N195F,   G182*+D183*+A186D,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K,   G182*+D183*+A186N,   H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K,   H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S244Q+K391A+G476K.   G182*+D183*+F328L,   G182*+D183*+N195F+A288V,   G182*+D183*+A186N+N195F,   G182*+D183*+E190P+N195F,   W140Y+G182*+D183*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+25V+K391A+G476K,   G182*+D183*+A186D+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+W48Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F+V238A,   G182*+D183*+Y295N,   Q172G+G182K+D183*,   F113Q+G182*+D183*+N195F,   H1*+N54S+V56T+C298A+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F+Y295F,   G182*+D183*+A186E.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206Y+K391A+G476K,   G182K+D183K+N195F+W284H,   G182*+D183*+A186Q+N195F.   K72R+G182*+D183*,   D183*+G182*A-N195F+V326L,   D183*+G182*+A186E+N195F,   Q169E+G182*+D183*+N195F,   D183*+G182*+N195F+K242Q,   G7A+G182*+D183K+N195F,   D183*+G182*+N195F+N260G,   D183A+G182*+R321A+S323N,   D183*+G182*+W284H,   H1*+D183*+G182*,   D183*+G182*+A186H+N195F,   D183*+G182*A+289V,   D183*+G182*+K391A,   D183*+G182*+S304R,   W167F+G182*+D183*+N195F.   F113Q+R116H+D183*+G182*,   D183*+G182*+N270G.   D183A+G182*+S244Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   D183*+G182*+N195F+Y295N,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K   D183*+G182*+A186Q,   D183*+G182*+N195F+V206F,   D183*+G182*+N195F+R320A,   G182*+D183*+G184T+N195F,   N54S+V56T+D183*+G182*,   W48Y+D183*+G182*,   A174S+D183*+G182*,   G7A+D183*+G182*,   N54S+D18S+G182*,   D183*+G182*+N195F+M246V,   W48Y+D183*+G182*+N195F,   D183*+G182*+N195F+S304R,
       wherein numbering is according to SEQ ID NO: 13, and wherein the IF is determined by use of Model A detergent.   
       

     In one embodiment, the IF is determined by use of Model A detergent at a temperature of 15° C. In a particular embodiment, the variants have an IF of &gt;1 as compared to the parent polypeptide, wherein the IF is determined by use of Model A detergent at a temperature of 15° C. and a concentration of 0.05 mg/L. It can be seen from the Tables in the Examples herein disclosed which such variants may be. 
     In another embodiment, the variant has an IF of at least 1.0 when compared to the parent polypeptide when tested in Model J detergent, and wherein the variant is selected from the group consisting of:
     G182*+D183*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+G142T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+M246T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K,   H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K,   H1*+N54S+V56T+G109A+W167I+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+G346S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182A+D183*+N195F+V206L+K391A+T444S+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+S280L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280T+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+G255N+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q449T+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391A+P473K+G476K,   H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K,   H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484E.   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391A+G476K+K484Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S,   H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183A+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+H324L+K391A+G476K,   H1*+N54S+V56T+G109A+G149Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A,   H1*+N54S+V56T+G109A+G255A++A174S+G182K+D183*+N195F+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q,   H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G423H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y,   H1*+T5K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391 Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K,   H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280K+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A.   H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G337H+Q385L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+Y382M+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*-M54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182K+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+M105F+G109A+Q118F+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+R320A+S323N+D377H+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K.   H1A+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+M195F+V206L+T355F+K391A+G476K.   H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K.   H1*-M54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174D+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+A174P+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+A174M+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D*+N195F+V206L+D377H+K391A+G476K,   H1*+Q11H+N54S+V56T+G109A+Q118R+T134E+A174S+G184I+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A,   H1*+N54S+V56T+K72A+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167S+G182*+D183*+N195F+A174S+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+W167S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72E+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184A+T193K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+V291G+D377H+K391A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K,   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184I+N195F+V206L+K391A+G47   6K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+V238A+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F328M+D377H+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328V+D377H+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K.   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K.   H1*+N54S+V56I+G109A+L173H+A174S+G182*+D183A+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1A+N16S+N54S+V56T+G109A+A174S+G182A+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72A+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183K+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*4N195F+G184T+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184I+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*4N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K,   H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346D+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K,   H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K,   H1*+N54S+V56T+G109A+R116I+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174F+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51D+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172R+A174S+K179E+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182 D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+A174G+G182A+D183A+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391A+K391S+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K,   H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+A225F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382I+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+I214E+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L-H214W+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+A204T+V206L+D377H+K391A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V26L+Y267I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K,   H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1A+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D18S+N195F+V206L+M24 D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K,   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54R+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54L+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391 W+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391I+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250 T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+L250F+D377H+K39A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Q+G476K,   H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56I+G109A+Q172E+L173A+A174R+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+K72Q+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184I+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173I+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+K391A+G476K,   H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K,   H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+L173V+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173T+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267I+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G12W+D*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K,   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182+D183*+N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A,   H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K,   H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V264I+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D15F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+67F+Q172R+A174S+G182*+D183*+N195F+V206L+F289I+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+I67F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R6H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184I+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+N5F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184I+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+\N167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A74S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+k 167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+\N167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L,   N54S+V56T+G109A+G182*+D183*+N195F,   N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K,   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L,   H1*+W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A,   H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+T165 G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+G476K.   G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391T+G476K,   H1A+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444 Q+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1A+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444 Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D*+N195F+V206L+M246F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K,   H1*+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   G109A+G182*+D183*+N195F+V206L+G476K,   H1*+G109A+G182*+D183*+N195F+K391A,   H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H,   H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K.   H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183K+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+P364A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1A+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K.   H1*+G7K+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T165V+A174S+G182K+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K,   H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   N54S+G182*+D183*+N195F,   G109A+G182*+D183*+N195F.   A174S+G182*+D183K+N195F,   G182*+D183*+N195F+K391A.   G182*+D183A+N195F+G476K,   H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K;   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q72K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K,   H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q72K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F,   H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K,   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174 G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K, F113Q+R116H+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   Q169E+Q172K+A174W+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   F113Q+R116Q+D183*+G182*+N195F,   N54S+V56T+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   Q169E+Q172K+G182*+D183*+N195F,   G182*+D183*+A186D,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K,   G182*+D183*+A186N,   H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K,   H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S244Q+K391A+G476K,   G182*+D183*+F328L,   G182*+D183*+N195F+A288V,   G182*+D183*+A186N+N195F,   G182*+D183*+E190P+N195F,   W140Y+G182*+D183*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225V+K391A+G476K,   G182*+D183*+A186D+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+W48Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F+V238A,   G182*+D183*+Y295N,   Q172G+G182*+D183*,   F113Q+G182*+D183*+N195F,   H1*+N54S+V56T+Q98A+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F+Y295F,   G182*+D183*+A186E,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206Y+K391A+G476K,   G182*+D183*+N195F+W284H,   G182*+D183*+A186Q+N195F,   K72R+G182*+D183*,   D183*+G182*+N195F+V326L,   D183*+G182*+A186E+N195F,   Q169E+G182*+D183*+N195F,   D183*+G182*+N195F+K242Q,   G7A+G182*+D183*+N195F,   D183*+G182*+N195F+N260G,   D183*+G182*+R321A+S323N,   D183*+G182*+W284H,   H1*+D183*+G182*,   D183*+G182*+A186H+N195F,   D183*+G182*+F289V,   D183*+G182*+K391A,   D183*+G182*+S304R,   W167F+G182*+D183*+N195F,   F113Q+R116H+D183*+G182*,   D183*+G182*+N270G,   D183*+G182*+S244Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   D183*+G182*+N195F+Y295N,   W48F+D183*+G182*,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   D183*+G182*+A186Q,   W140Y+D183*+G182*,   D183*+G182*+K242Q,   D183*+G182*+V206L,   D183*+G182*+N195F+V206F,   Q169E+D183*+G182*,   D183*+G182*+N195F+R320A,   G182*+D183*+G184T+N195F,   D183*+G182*+G184T,   D183*+G182*+P473R,   W48Y+D183*+G182*,   D183*+G182*+R323A,   G7A+D183*+G182*,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   A174S+D183*+G182*,   D183*+G182*+N195F+S323N,   D183*+G182*+N195F+M246V,   W48Y+D183*+G182*+N195F,   V56T+D183*+G182*,
 
wherein numbering is according to SEQ ID NO: 13,
   G182*+D183*+N195F+A428T,   G182*+D183*+N195F,   H1*+G182*+D183*+N195F,   N54S+G182*+D183*+N195F,   V56T+G182*+D183*+N195F,   G182*+D183*+N195F+I405L,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+D476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477S;   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K,   H1*+N54S+V56T+K72R+G109A+\Ail 67F+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L&#39;S280Q+H321Y+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S304N+I405L+A421H+A422P+A428T+D476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G+G477T,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422   P+A428T+G477A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476N,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476Y,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206I+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+G196R+V206L+G255A+I405L+A421H+A422P+A428T+N475S,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116Q+G133Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+G448D,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*D183*+N195F+V206L+S+A421H+A422P+A428T,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+M105F+G182*+D183*+N195F+V206L+L228I+R320A+S323N+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+O172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14.   

     In one embodiment, the IF is determined by use of Model J detergent at a temperature of 15° C. In a particular embodiment, the variants have an IF of &gt;1 as compared to the parent polypeptide, wherein the IF is determined by use of Model J detergent at a temperature of 15° C. and a concentration of 0.05 mg/L. It can be seen from the Tables in the Examples herein disclosed which such variants may be. 
     In another embodiment, the variant has an IF of at least &gt;1.5 when compared to said parent polypeptide, and wherein said variant is selected from
     H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+G142T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K,   H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K,   H1*+N54S+V56T+G109A+W167I+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255N+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q449T+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473K+G476K,   H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K,   H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484E,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S,   H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+H324L+K391A+G476K,   H1*+N54S+V56T+G109A+G149Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A,   H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q,   H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G423H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y,   H1*+T5K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K,   H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K   H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280K+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A,   H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+I134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G337H+Q385L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+Q118F+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K,   H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I355L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+A174P+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72A+G109A+W167S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+Q11H+N54S+V56T+G109A+Q118R+T134E+A174S+G184I+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A,   H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+D377H+K391A+G476K;   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328V+D377H+K391A+G476K;   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291T+D377H+K391A+G476K;   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K;   H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N16S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72A+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182A+D183*+N195F+G184T+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K,   H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217 Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K,   H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K,   H1*+N54S+V56T+G109A+R116I+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174F+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+I51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*4D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51D+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172R+A174S+K179E+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391S+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391A+T444A+G476K,   H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183A+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382I+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+I214W+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+D377H+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T16 S A174S+G182A+D195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N5F+V206L+M286F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182A+D183A+N195F+V206L+L235V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K,   H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K A+G476K,   H1*+N54S+V56T+M105V+G109A+A174S+G182*4D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K,   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54L+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391I+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Q+G476K,   H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+W167R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376I+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173I+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+K391A+G476K,   H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K,   H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+L173V+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173T+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*4D183*+N195F+i-\204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K,   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A,   H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K,   H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V264I+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+F113Q+R6H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F289I+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R6H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+\N167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D1*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182 D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172N+A74S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N5F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+67F+Q172N+A174S+G182 D183*+N195F+V206L+K391A+T459N+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F V206L+K391A+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+I173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L,   N54S+V56T+G109A+G182*+D183*+N195F,   N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*4N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K,   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L,   H1*+W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+G476K,   G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   N54S+V56I+G109A+G182*+D183*+N195F+V206L+K391T+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+K391A+G476K,   H1*+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   G109A+G182*+D183*+N195F+V206L+G476K.   H1*+G109A+G182K+D183*+N195F+K391A,   H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H,   H1A+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182A+D18S+N195F+V206L+K391A+G476K.   H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+P364A+K391A+C474V+G476K.   H1A+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+G184T+N195F+V206L+K391A+G476K,   H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+Y382L+K391A+G476K,   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174N+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K,   H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   G109A+G182*+D183*+N195F,   G182*+D183*+N195F+K391A,   H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R6Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184I+N195F+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K,   H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F,   H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K,   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K,   F113Q+R116H+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   Q169E+Q172K+A174*+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   F113Q+R116Q+D183*+G182*+N195F,   N54S+V56T+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   Q169E+Q172K+G182*+D183*+N195F,   G182*+D183*+A186D,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K,   G182*+D183*+A186N,   H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K,   H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   G182*+D183*+A186N+N195F,   G182*+D183*+A186D+N195F,   G182*+D183*+N195F+W284H,   D183*+G182*+N195F+V326L,
 
wherein numbering is according to SEQ ID NO: 13, and
   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G+G477T,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476N,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476Y,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206I+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+T40D+N54S+V56T+G09A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G195F+V296L+I405L+A421H+A422P+A428T,   H1*+N54S+V56I+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G173V+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I4+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+F116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183* N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14, and wherein the IF is determined by use of Model A detergent.   

     In one embodiment, the IF is determined by use of Model A detergent at a temperature of 15° C. In a particular embodiment, the variants have an IF of &gt;1.5 as compared to the parent polypeptide, wherein the IF is determined by use of Model A detergent at a temperature of 15° C. and a concentration of 0.05 mg/L. It can be seen from the Tables in the Examples herein disclosed which such variants may be. 
     In another embodiment, the variant has an IF of at least 1.5 when compared to the parent polypeptide and when determined by use of Model J detergent, wherein the variant is selected from the group consisting of:
     G182*+D183*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T40 G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37Y+N54S+V56I+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+G142T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K,   H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118K+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K,   H1+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K,   H1*+N54S+V56T+G109A+W167I+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K,   H1+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K,   H1+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444 Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K,   H1+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255N+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q449T+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473K+G476K,   H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K,   H1*+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484E,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S,   H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+H324L+K391A+G476K,   H1*+N54S+V56T+G109A+G149Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A,   H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q,   H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G423H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y,   H1*+T5K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K,   H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280K+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A,   H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G337H+Q385L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+Q118F+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K,   H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355F+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+V56T+M105k-G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+A174M+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+Q11H+N54S+V56T+G109A+Q118R+T134E+A174S+G184T+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*4N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A,   H1*+N54S+V56T K72A+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167S+G182*+D183*+N195F+A174S+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T K72R+G109A+\A/167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291 G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K,   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G47   6K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328H-D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291 T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N16S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72A+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K,   H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346D+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K,   H1*+N54S+V56T+G109A+I134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K,   H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K,   H1*+N54S+V56T+G109A+R116I+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174F+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51D+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174Q+G182*4D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391S+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K,   H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382I+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204S+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N5F+V206L+L235A+D377H+K391A+G476K,   H1*+N54S+V56T+M105L+G109A+A174S+G182 D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+M246I+D377H+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K,   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K.   H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174L+G182*+D183*+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391D+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391I+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L L250M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Q+G476K,   H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173A+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A-1-W167R-1-A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184I+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173I+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+K391A+G476K,   H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+K391A+G476K,   H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+L173V+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173 T+A174R+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D1*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+L250F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+A204S+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I334S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+Y267I+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+K391L+G476K   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1A+N54S+V56T+K72R+G109A+T134E+A174S+G182A+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K.   H1A+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K,   H1A+N54S+V56T+G109A+I134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+D377H+K391A+C474V+G476K   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+G476K+G477A,   H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K.   H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182K+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183A+N195F+V206L+G346T+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V264I+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321 Y+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F289I+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1+N54S+V56T+G109A+F113Q+R H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K,   H1+N54S+V56T+G109A+F113Q+R H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+i-\265G+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56I+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+67F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*4D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L,   N54S+V56T+G109A+G182*+D183*+N195F,   N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K,   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L,   H1*+W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A,   H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+G476K,   G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391T+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+I165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1A+A37H+N54S+V56T+G109A+R116H+T165 G+A174 S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K,   H1*+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   G109A+G182*+D183*+N195F+V206L+G476K.   H1*+G109A+G182*+D183A+N195F+K391A,   H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H,   H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K.   H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183K+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+P364A+K391A+C474V+G476K.   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1A+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+Y382L+K391A+G476K,   H1*+G7K+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L K391A+G476K.   H1A+N54S+V56T+G109A+W167H+A174S+G182A+D183A+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T165V+A174S+G182K+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174N+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K,   H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   N54S+G182*+D183*+N195F,   G109A+G182*+D183*+N195F,   G182*+D183*+N195F+G476K,   H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G478K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K,   H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F,   H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K,   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K,   F113Q+R116H+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   Q169E+Q172K+A174*+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   F113Q+R116Q+D183*+G182*+N195F,   N54S+V56T+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*4N195F+V206L+K391A+G476K,   Q169E+Q172K+G182*+D183*+N195F,   G182*+D183*+A186D,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K,   G182*+D183*+A186N,   H1*+N54S+V56T+G109A+A174S+V206L+G182*+D183*+K391A+G476K,   H1*+N54S+V56T+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F289L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y295F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S244Q+K391A+G476K,   G182*+D183*+F328L,   G182*+D183*+N195F+A288V,   G182*+D183*+A186N+N195F,   G182*+D183*+E190P+N195F,   W140Y+G182*+D183*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A225V+K391A+G476K,   G182*+D183*+A186D+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+W48Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F+V238A,   G182*+D183*+Y295N,   Q172G+G182*+D183*,   F113Q+G182*+D183*+N195F,   H1*+N54S+V56T+Q98A+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F+Y295F,   G182*+D183*+A186E,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206Y+K391A+G476K,   G182*+D183*+N195F+W284H,   G182*+D183*+A186Q+N195F,   K72R+G182*+D183*,   D183*+G182*+N195F+V326L,   D183*+G182*+A186E+N195F,   Q169E+G182*+D183*+N195F,   D183*+G182*+N195F+K242Q,   G7A+G182*+D183*+N195F,   D183*+G182*+N195F+N260G,   D183*+G182*+R321A+S323N,   D183*+G82*+W284H,   D183*+G182*+A186H+N195F,   D183*+G182*+K391A.   wherein numbering is according to SEQ ID NO: 13,   V56T+G182*+D183*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G INA-FRI 74S+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+G182*+D163*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477S,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476G+G477T,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+G477A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476K+G477Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476N,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+I405L+A421H+A422P+A428T+D476Y,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206I+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*4N195F+G196R+V206L+G255A+405L+A421H+A422P+A428T+N475S,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T.   H1*+N54S+V56T+G109A+R116Q+G133Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+G448D,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+i-\265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A74S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172G+A174S+G D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+A174S+M105F+G182*+D183*+N195F+V206L+L228I+R320A+S323N+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1+A37H+N54S+V56T+A60V+G109A+R116Q+\N167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14.   

     In one embodiment, the IF is determined by use of Model J detergent at a temperature of 15° C. In a particular embodiment, the variants have an IF of &gt;1.5 as compared to the parent polypeptide, wherein the IF is determined by use of Model J detergent at a temperature of 15° C. and a concentration of 0.05 mg/L. It can be seen from the Tables in the Examples herein disclosed which such variants may be. 
     Some variants according to the invention have an even further improved wash performance indicated by an IF of at least 2.0 when compared the a parent polypeptide having an amino acid sequence set forth in SEQ ID NO: 15, and wherein the IF is determined by use of Model A detergent at a temperature of 15° C. 
     Thus, in one embodiment, the variant has an IF of at least &gt;2.0 when compared to said parent polypeptide, and wherein said variant is selected from
     H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K,   H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172R+A174S+G182*4D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K,   H1*+G50A+N54S+V56T+G109A++C) 172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S,   H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A,   H1*+N54S+V56T+G109AG255A++A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y,   H1*+N54S+V56T+G109A+R116M+A174S+G182K+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+I214L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+P211D+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444R+G476K,   H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K.   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+R320A+K391A+G476K   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A,   H1*+N54S+V56T+G109A+Q118S+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+Y382M+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K.   H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1A+N54S+V56T+M105F+G109A+I134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q299V+K391A+G476K   H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T355L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1A+N54S+V56T+K72R+G109A+A174S+G182A+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183A+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A74S+G182*+D183A+G184T+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72N+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K, H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K,   H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116I+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G2*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K721V1+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1A+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+S323N+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382I+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+A204S+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K,   H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1A+N54S+V56T+M105I+G109A+A174S+G182*+D183*+N195F V206L+D377H+K391A+G476K,   H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391V+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+K391M+G476K,   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174V+G182*+D183A+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+M246V+D377H+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391W+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183+N195F+V206L+K391I+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+L250A+D377H+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183+N195F+V206L+L250M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q172H+L173A+A174P+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+S376T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376V+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173I+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+K391A+G476K,   H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G476K,   H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K,   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391L+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+04G+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K,   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183A+N195F+V206L+R320A+S323N+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182+D183*4N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+A265G+K391A+T444Q+G476K+G477A,   H1*+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K,   H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183A+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*4N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V264I+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+F289I+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R6H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N19F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182 D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D1*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182 D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+73V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+I444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183A+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183+N195F+V206L+K391A+T459N+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183A+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+k 167F+Q172R+A174S+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+T165 G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L,   N54S+V56T+G109A+G182*+D183*+N195F,   N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K,   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L,   N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116C)+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+V56A+G109A+A174S+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+\N167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K,   H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H,   H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K,   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K,   H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K,   H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G2 S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,
 
wherein numbering is according to SEQ ID NO: 13, and
   H1*+T40D+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T   H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T   H1*+N54S+V56T+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D183*+G184T+N195F+V206L+I405L+A421H+A422P+A428T   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183F+V206L+A265G+I405L+A421H+A422P+A428T   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14, and wherein the IF is determined by use of Model A detergent.   

     In one embodiment, the variant has an IF of at least 2.0 when compared to the parent polypeptide, wherein the IF is determined by use of Model A detergent at a temperature of 15° C. and a concentration of 0.05 mg enzyme/L. 
     In one embodiment, the variant has an IF of at least 2.0 when compared to the parent polypeptide and when determined by use of Model J detergent, and wherein the variant is selected from the group consisting of:
     H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T40G+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37M+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37Y+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37R+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125P+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V213T+K391A+G476K,   H1*+A37L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377R+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214G+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S381A+K391A+G476K,   H1*+N54S+V56T+G109A+W167I+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420S+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420K+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+A420L+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473T+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444S+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391A+T444Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280W+K391A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+S280T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255N+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T285L+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+T285Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473K+G476K,   H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+T40K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K.   H1A+G50A+N54S+V56T+G109A++Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484G   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484P.   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391A+G476K+K484E,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484Q   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+K484S.   H1*K+G50A+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+Q98S+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+H324L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A,   H1*+N54S+V56T+G109AG255A++A174S+G182K+D183*+N195F+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+F113L+R116L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K+G477Q,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q345D+K391A+G476K+G477Q,   H1*+G50A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G423H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320V+K391A+G476Y,   H1*+N54S+V56T+G109A+R116M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P211D+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+K391Q+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444V+G476K,   H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+Q118G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382M+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*4N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+M105F+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+D377H+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173A+A174S+G182*4D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+Q299V+K391A+G476K,   H1*+N54S+V56T+G109A+L173G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I355L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+A174S+G182*+D183K+N195F+A204T+V206L+K391A+G476K.   H1*+G50A+N54S+V56T+G109A+A174E+G182*+D183*+N195F+A204T+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+T51G+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1A+Q11H+N54S+V56T+G109A+Q1R T134E+A174S+G184T+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183.*+G184T+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1A+N54S+V56T+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A74S+G182*+D183A+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A,   H1*+N54S+V56T+K72R+G109A+W167L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183+N195F+V206L+G255A+D377H+K391A+G476K   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L V238G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+F328I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D1*+G184T+N195F V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+L173H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N16S+N54S+V56T+G109A+A174S+G182*+D183*4N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72N+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+G184T+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+A174S+G182*+D183*+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L217T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377S+K391A+G476K,   H1*+N54S+V56T+G109A+R116E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377A+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G346P+K391A+G476K,   H1*+N54S+V56T+G109A+R116S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377Q+K391A+G476K,   H1*+N54S+V56T+G109A+R116I+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51S+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51A+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51E+N54S+V56T+G109A+Q172L+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72H+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72M+G109A+Q172S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72Q+G109A+A174G+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72S+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444A+G476K,   H1*+N54S+V56T+G109A+F113L+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214E+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+I214W+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+A204T+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+A204S+V206L+D377H+K391A+G476K.   H1A+N54S+V56T+G109A+I165S+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M286F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235V+D377H+K391A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L235A+D377H+K391A+G476K,   H1*+N54S+V56T+M105L+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+M105I+G109A+A174S+G182*+D183.*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246I+D377H+K391A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250I+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183+N195F+V206L L250V+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391Y+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183+N195F+V206L+K391V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391M+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391E+G476K,   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109S+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174V+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182A+D183+N195F+V206L+M246F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174L+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+K391D+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391R+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391H+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+K391W+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391I+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250T+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+L250A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q172H+L173A+A74P+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183+G184T+N195F+V206L+G255A+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S376T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+S376V+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173I+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+L173A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267M+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183+N195F+V206L+Y267H+D377H+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+V238G+K391A+G476K,   H1*+N54S+V56T+M105V+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264T+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264I+K391A+G476K.   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+L235V+K391A+G476K,   H1*+N54S+V56T+G109A+W167H+Q172N+L173A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+L173T+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173F+A174R+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109A+Q172E+L173T+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+L173V+A174R+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173M+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172E+L173F+A174P+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+Q172E+L173V+A174H+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+L173A+A174T+G182K+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V291A+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+L250V+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250F+K391A+G476K.   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+T334S+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A263G+M286L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267H+K391A+G476K,   H1A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y267L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182K+D183*+N195F+V206L+K391L+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*4N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+G184T+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473G+G476K,   H1*+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183K+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183A+N195F+V206L+R320A+S323N+K391A+C474V+G476K.   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*4N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183A+N195F+V206L+A265G+K391A+T444Q+G476K+G477A.   H1A+A37V+N54S+V56T+G109A+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G346T+G477A+G476K,   H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G346I+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109S+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V264I+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R6Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+R116H+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D1*+N195F+V206L+F289I+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+Q395P+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+A265G+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172R+A174S+G182*+D183*+N195F+V2   06L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+T51V+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+S280Q+H321Y+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R6H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+A445Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+A37H+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+\Ail 67F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+T459N+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172R+L173V+A74S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G478K,   H1*+A37V+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116A+W167F+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+G182*+D N195F+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L,   N54S+V56T+G109A+G182*+D183*+N195F,   N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+G476K,   H1*+N54S+V56T+A174S+G182*+D183*+N195F+V206L+G273R+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L,   H1*+W48G+G109A+G182*+D183*+N195F+V206L+K391A+G476K,   N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A,   H1*+N54S+V56T+G109A+F113Q+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D1+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+V56A+G109A+A174S+G182*+D183*4N195F+V206L+G476K,   H1*+N54S+V56T+G109A+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+67F+Q172R+A174S+G182*+D1*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+R116H+Q172R+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+K391A+G476K,   G109A+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+G133S+A174S+G182*+D183*+N195F+K391A+G476H,   H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+P124A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+P364A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382L+K391A+G476K,   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W167H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T165V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174N+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174Q+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Y382F+K391A+G476K,   H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*4N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q72K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+A186D+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A225V+K391A+G476K,   H1*+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255S+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   G182*+D183*+N195F,   H1*+N54S+V56T+G109A+Q169E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+W284H+K391A+G476K,   H1*+N54S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+S304R+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+M246V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295F+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+R320A+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+W140Y+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+F328L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G476K,   F113Q+R116H+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+A288L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270G+K391A+G476K,   Q169E+Q172K+A174*+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K269Q+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   F113Q+R116Q+D183*+G182*+N195F,   N54S+V56T+D183*+G182*+N195F,   H1*+N54S+V56T+G109A+A174T+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116W+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   Q169E+Q172K+G182*+D183*+N195F,   G182*+D183*+A186D,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238A+K391A+G476K,   G182*+D183*+A186N,
 
wherein numbering is according to SEQ ED NO: 13,
   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206I+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+G196R+V206L+G255A+I405L+A421H+A422P+A428T+N475S,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+N125D+A174S+G182+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+R116Q+G133Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T+G448D;   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182 D183*+N195F+V206L+G255A+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+K72R+G109A+R116Q+V120L+A174S+G182*+D1 G184T+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A26   5G+I405L+A421H+A422P+A428T,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14.   

     In one embodiment, the variant has an IF of at least 2.0 when compared to the parent polypeptide, wherein the IF is determined by use of Model J detergent at a temperature of 15° C. and an enzymes concentration of 0.05 mg/L. 
     Other variants according to the invention have an IF of at least 3.0, indicating that at least these variants have a significantly improved wash performance when compared the a parent polypeptide having an amino acid sequence set forth in SEQ ID NO: 15. 
     In a particular embodiment, the variant is selected from a group consisting of:
     H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K, and   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K, wherein numbering is according to SEQ ID NO: 13.   

     In another embodiment, the variant has an IF of at least 3.0 when compared to the parent polypeptide and determined by use of Model J detergent, and wherein the variant is selected from the group consisting of:
     H1*+T51K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q125A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+P473A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+T444D+G476K,   H1*+N54S+V56T+K72S+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+G50A+N54S+V56T+G109A+L173V+A174S+G182*+D183*+N195F+A204V+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+G476K+G477A,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+G184T+N195F V206L+K391A+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+D377H+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174Q+G182*+D183*+N195F+V206L+K391A+G476K+G477A,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*4N195F+G184T+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V238G+K391A+G476K,   H1A+N54S+V56T+G109A+W167F+Q172E+L173P+A174K+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+L250M+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K.   H1*+N54S+V56T+G109S+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q172D+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+A204G+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+W167F+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+M105F+G109A+A174S+G182*+D183*+N195F+V206L+R320A+S323N+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+T134E+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+D377H+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+R116Q+Q172D+A174S+G182*+D183*+N195F+V206L+G346T+K391A+T444Q+G477A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172N+A174S+G182*+D183A+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+G109S+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172G+A174S+G182*4D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q172N+A174S+G182*+D183*+N195F+V206L+V264I+K391A+C474V+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+T165G+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+Q172N+A174S+G182*+D183*+N195F+V206L K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+Q172M+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+T165G+A174S+G182*+D1+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R6H+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116Q+W167F+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+G133Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+Q395P+K391A+G476K,   H1*+A37V+N54S+V56T+K72R+G109A+R116H+W167F+Q172R+A174S+G182 D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109S+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109S+F113Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109S+F113Q+W167F+Q172R+A174S+G182*+D183*N195F+V206L+K391A+G476K   H1*+N54S+V56T+K72R+G109A+R116H+T134E+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+G255A+K391A+Q395P+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+T134E+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+G255A+K391A+T444Q+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+T134E+W167F+Q172N+A174S+G182K+D183*+N195F+V206L+G255A+A265G+K391A+T444Q+P473R+G476K,   H1+N54S+V56T+K72R+G109A+G133Q+W167F+Q172R A74S+G182*+D183A+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+N195F+A204G+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+A174S+G182*+D183*+N195F+V206L K391A+P473R+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116H+Q172R+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+A60V+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+P473R+G476K,   H1+N54S+V56T+A60V+G109A+R116Q+W167F+Q172N+L173V A74S+G182A+D183*+N195F+V206L+K391A+G476K,   H1A+N54S+V56T+A60V+G109A+R116Q+W167F+Q172R+A174S+G182*+D183*+N195F+V206L+K391A+G476K   H1*+N54S+V56T+G109A+R116A+A174S+G182*+D183*+N195F+A204G+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+T134E+A174S+G182*+D183*+N195F+V206L+G255A+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+R116Q+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116A+T165G+A174S+G182*+D183*4N195F+V206L+K391A+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+A174S+G182*+D183*+G184T+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109S+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+T444Q+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+W167F+Q172G+L173V+A174S+G182*+D183*+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+K391A+P473R+G476K,   H1*+A37V+N54S+V56T+G109A+R116Q+T165G+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+A37H+N54S+V56T+G109A+R116H+T165G+A174S+G182*+D183*+N195F+V206L+M246F+K391A+G476K,   H1*+N54S+V56T+G109A+R116A+Q172N+L173V+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+P45A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+C474V+G476K,   H1*+N54S+V56T+G109A+Q118R+T134E+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+G50A+T51A+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+V264F+K391A+G476K,   H1*+G7K+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+G255A+Q256A+K391A+G476K,   H1*+N54S+V56T+G109A+W167Y+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N16H+V17L+N54S+V56T+G109A+A174S+G182A+D183*+N195F+V206L+Y382F+K391A+G476K,   H1*+Q32S+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+Q385L+K391A+G476K,   H1*+N54S+V56T+R87S+G109A+R171H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+R116H+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R6Q167F+Q172G A174S+G182*+D183*+G184T+N195F+V206L+P473R+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172G+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116Q+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+R116W+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206F+K391A+G476K,   H1*+V56T+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+G184T+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+Y295N+K391A+G476K,   H1*+N54S+V56T+G109A+W167F+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+A186N+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+A288V+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G255A+K391A+G476K,   H1*+N54S+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+W48F+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q72K+A174*+G182*+D183*+N195F+V206L+V291A+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+A174S+G182*+D183*+N195F+V206L+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+R320A+S323N+K391A+G476K,   H1*+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F V206L+K391A+G476K,   H1*+N54S+V56T+K72R+G109A+F113Q+R116H+W167F+Q172G+A174S+G182*+D183*+G184T+N195F+V206L+K391A+P473R+G476K,   H1*+N54S+V56T+G109A+A174S+G182*+D183*+N195F+V206L+N270T+K391A+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+G476K,   H1*+N54S+V56T+G109A+Q169E+Q172K+A174*+G182*+D183*+N195F+V206L+N260G+K391A+G476K,   H1*+N54S+V56T+G109A+F113Q+R116H+A174S+G182*+D183*+N195F+V206L+K391A+G476K,
 
wherein numbering is according to SEQ ID NO: 13,
   H1*+N54S+V56T+G109A+F113Q+R116H+Q172G+A174S+G182*+D183*+N195F+V206L+I405L+A421H+A422P+A428T,   H1*+N54S+V56T+G109A+F113Q+R116Q+Q172N+A174S+G182*+D183*+N195F+V206L+A265G+I405L+A421H+A422P+A428T, wherein numbering is according to SEQ ID NO: 14.   

     The variants according to the present invention may further comprise one or more additional alterations than those described above at one or more (e.g., several) other positions. 
     The amino acid changes may be of a minor nature, that is conservative amino acid substitutions or insertions that do not significantly affect the folding and/or activity of the protein: small deletions, typically of 1-30 amino acids; small amino- or carboxyl-terminal extensions, such as an amino-terminal methionine residue; a small linker peptide of up to 20-25 residues; or a small extension that facilitates purification by changing net charge or another function, such as a poly-histidine tract, an antigenic epitope or a binding domain. 
     Examples of conservative substitutions are within the groups of basic amino acids (arginine, lysine and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagine), hydrophobic amino acids (leucine, isoleucine and valine), aromatic amino acids (phenylalanine, tryptophan and tyrosine), and small amino acids (glycine, alanine, serine, threonine and methionine). Amino acid substitutions that do not generally alter specific activity are known in the art and are described, for example, by H. Neurath and R. L. Hill, 1979,  in, The Proteins , Academic Press, New York. Common substitutions are Ala/Ser, Val/Ile, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr, Ser/Asn, Ala/Val, Ser/Gly, Tyr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/Ile, Leu/Val, Ala/Glu, and Asp/Gly. 
     Alternatively, the amino acid changes are of such a nature that the physico-chemical properties of the polypeptides are altered. For example, amino acid changes may improve the thermal stability of the polypeptide, alter the substrate specificity, change the pH optimum, and the like. 
     Essential amino acids in a polypeptide may be identified according to procedures known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis (Cunningham and Wells, 1989 , Science  244: 1081-1085). In the latter technique, single alanine mutations are introduced at every residue in the molecule, and the resultant mutant molecules are tested for alpha-amylase activity to identify amino acid residues that are critical to the activity of the molecule. See also, Hilton et al., 1996 , J. Biol. Chem.  271: 4699-4708. The active site of the enzyme or other biological interaction can also be determined by physical analysis of structure, as determined by such techniques as nuclear magnetic resonance, crystallography, electron diffraction, or photoaffinity labeling, in conjunction with mutation of putative contact site amino acids. See, for example, de Vos et al., 1992 , Science  255: 306-312; Smith et al., 1992 , J. Mol. Biol.  224: 899-904; Wlodaver et al., 1992 , FEBS Lett.  309: 59-64. The identity of essential amino acids can also be inferred from an alignment with a related polypeptide. 
     The variants according to the present invention may consist of 400 to 485 amino acids, e.g., 410 to 485, 425 to 485, and 440 to 485 amino acids. 
     Polynucleotides 
     The present invention also relates to polynucleotides encoding a variant of the present invention. Thus, in particular, the present invention relates to a polynucleotide endocing a variant comprising an alteration in one or more positions corresponding to the H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40. N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280. W284, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, G448, D476, and G477 of SEQ ID NO: 14. 
     The term “polynucleotides encoding” as used herein, refers to a polynucleotide that encodes a mature polypeptide having alpha-amylase having alpha-amylase activity. In one aspect, the polypeptide coding sequence is the nucleotide sequence set forth in SEQ ID NO: 1, 2, or 3. 
     In one embodiment, the polynucleotide encoding a variant according to the present invention as at least 60%, such as at least 65%, such as at least 70%, such as at least 75%, such as at least 80%, such as at least 85%, such as at least 90%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99% but less than 100% sequence identity to the polynucleotide of SEQ ID NOs: 1, 2 and 3. 
     Nucleic Acid Constructs 
     The present invention also relates to nucleic acid constructs comprising a polynucleotide encoding a variant of the present invention operably linked to one or more control sequences that direct the expression of the coding sequence in a suitable host cell under conditions compatible with the control sequences. Thus, in particular, the present invention relates to a nucleic acid construct comprising a polynucleotide encoding a variant comprising an alteration in one or more positions corresponding to the positions H1 T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, wherein the polynucleotide is operately linked to one or more control sequences. 
     The term “nucleic acid construct” as used herein, refers to a nucleic acid molecule, either single- or double-stranded, which is isolated from a naturally occurring gene or is modified to contain segments of nucleic acids in a manner that would not otherwise exist in nature or which is synthetic, which comprises one or more control sequences. 
     The term “operably linked” as used herein, refers to a configuration in which a control sequence is placed at an appropriate position relative to the coding sequence of a polynucleotide such that the control sequence directs expression of the coding sequence. 
     The polynucleotide may be manipulated in a variety of ways to provide for expression of a variant. Manipulation of the polynucleotide prior to its insertion into a vector may be desirable or necessary depending on the expression vector. The techniques for modifying polynucleotides utilizing recombinant DNA methods are well known in the art. 
     The control sequence may be a promoter, a polynucleotide which is recognized by a host cell for expression of the polynucleotide. The promoter comprises transcriptional control sequences that mediate the expression of the variant. The promoter may be any polynucleotide that shows transcriptional activity in the host cell including mutant, truncated, and hybrid promoters, and may be obtained from genes encoding extracellular or intracellular polypeptides either homologous or heterologous to the host cell. 
     Expression Vectors 
     The present invention also relates to recombinant expression vectors comprising a polynucleotide encoding a variant of the present invention, a promoter, and transcriptional and translational stop signals. Thus, the present invention relates to an expression vector, optionally recombinant, comprising a polynucleotide encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, a promoter, and transcriptional and translational stop signals. 
     The term “expression vector” as used herein, refers to a linear or circular DNA molecule that comprises a polynucleotide encoding a variant and is operably linked to control sequences that provide for its expression. 
     The various nucleotide and control sequences may be joined together to produce a recombinant expression vector that may include one or more convenient restriction sites to allow for insertion or substitution of the polynucleotide encoding the variant at such sites. Alternatively, the polynucleotide may be expressed by inserting the polynucleotide or a nucleic acid construct comprising the polynucleotide into an appropriate vector for expression. In creating the expression vector, the coding sequence is located in the vector so that the coding sequence is operably linked with the appropriate control sequences for expression. 
     The recombinant expression vector may be any vector (e.g., a plasmid or virus) that can be conveniently subjected to recombinant DNA procedures and can bring about expression of the polynucleotide. The choice of the vector will typically depend on the compatibility of the vector with the host cell into which the vector is to be introduced. The vector may be a linear or closed circular plasmid. 
     The vector may be an autonomously replicating vector, i.e., a vector that exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g., a plasmid, an extrachromosomal element, a minichromosome, or an artificial chromosome. The vector may contain any means for assuring self-replication. Alternatively, the vector may be one that, when introduced into the host cell, is integrated into the genome and replicated together with the chromosome(s) into which it has been integrated. Furthermore, a single vector or plasmid or two or more vectors or plasmids that together contain the total DNA to be introduced into the genome of the host cell, or a transposon, may be used. 
     The vector preferably contains one or more selectable markers that permit easy selection of transformed, transfected, transduced, or the like cells. A selectable marker is a gene the product of which provides for biocide or viral resistance, resistance to heavy metals, prototrophy to auxotrophs, and the like. 
     The skilled person would know which expression vector is the most suitable for specific expression systems. Thus, the present invention is not limited to any specific expression vector, but any expression vector comprising the polynucleotide encoding a variant according to the invention is considered part of the present invention. 
     Host Cells 
     The present invention also relates to recombinant host cells, comprising a polynucleotide encoding a variant of the present invention operably linked to one or more control sequences that direct the production of a variant of the present invention. Thus, the present invention relates to a host cell, optionally a recombinant host cell, comprising a polynucleotide encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, operably linked to one or more control sequences that direct the production of the variant. 
     The term “host cell” as used herein, refers to any cell type that is susceptible to transformation, transfection, transduction, or the like with a nucleic acid construct or expression vector comprising a polynucleotide of the present invention. The term “host cell” encompasses any progeny of a parent cell that is not identical to the parent cell due to mutations that occur during replication. 
     A construct or vector comprising a polynucleotide is introduced into a host cell so that the construct or vector is maintained as a Chromosomal integrant or as a self-replicating extra-chromosomal vector as described earlier. The choice of a host cell will to a large extent depend upon the gene encoding the variant and its source. 
     The host cell may be any cell useful in the recombinant production of a variant, e.g., a prokaryote or a eukaryote. 
     The prokaryotic host cell may be any Gram-positive or Gram-negative bacterium. Gram-positive bacteria include, but are not limited to,  Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus , and  Streptomyces . Gram-negative bacteria include, but are not limited to,  Campylobacter, E. coli, Flavobacterium, Fusobacterium, Helicobacter, ilyobacter, Neisseria, Pseudomonas, Salmonella , and  Ureaplasma.    
     Methods according to the Invention 
     The present invention also relates to methods of producing a variant, comprising: (a) cultivating a host cell of the present invention under conditions suitable for expression of the variant; and (b) recovering the variant. Thus, the present invention relates to a method of producing a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, P116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, wherein the method comprises the steps of a) cultivating a host cell according to the invention under conditions suitable for expression of the variant, and b) recovering the variant. 
     The host cells are cultivated in a nutrient medium suitable for production of the variant using methods known in the art. For example, the cell may be cultivated by shake flask cultivation, or small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium and under conditions allowing the variant to be expressed and/or isolated. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection), If the variant is secreted into the nutrient medium, the variant can be recovered directly from the medium. If the variant is not secreted, it can be recovered from cell lysates. 
     The variant may be detected using methods known in the art that are specific for the polypeptides having alpha amylase activity. These detection methods include, but are not limited to, use of specific antibodies, formation of an enzyme product, or disappearance of an enzyme substrate. For example, an enzyme assay may be used to determine the activity of the variant. 
     The variant may be recovered using methods known in the art. For example, the variant may be recovered from the nutrient medium by conventional procedures including, but not limited to, collection, centrifugation, filtration, extraction, spray-drying, evaporation, or precipitation. 
     The variant may be purified by a variety of procedures known in the art including, but not limited to, chromatography (e.g., ion exchange, affinity, hydrophobic, chromatofocusing, and size exclusion), electrophoretic procedures (e.g., preparative isoelectric focusing), differential solubility (e.g.; ammonium sulfate precipitation), SDS-PAGE, or extraction (see, e.g.,  Protein Purification , Janson and Ryden, editors, VCH Publishers, New York, 1989) to obtain substantially pure variants. 
     In an alternative aspect, the variant is not recovered, but rather a host cell of the present invention expressing the variant is used as a source of the variant. 
     Furthermore, the present invention relates to methods for obtaining a variant, comprising introducing into a parent alpha-amylase having at least 80% sequence identity to the polypeptide of SEQ ID NO: 4
         (a) a substitution and/or deletion of two or more positions in the parent alpha-amylase said positions corresponding to positions 181, 182, 183, and 184 of the mature polypeptide of SEQ ID NO: 4; and   (b) an alteration at one or more positions corresponding to positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14,   wherein the resulting variant has at least 80%, such as at least 85%, such as at least 90%, such as at least 95%, such as at least 97%, but less than 100% sequence identity with the mature polypeptide of SEQ ID NO: 4, wherein said variant has alpha-amylase activity;       

     (c) recovering said variant. 
     The variants may be prepared using any mutagenesis procedure known in the art, such as site-directed mutagenesis, synthetic gene construction, semi-synthetic gene construction, random mutagenesis, shuffling, etc. 
     Site-directed mutagenesis is a technique in which one or more (e.g., several) mutations are introduced at one or more defined sites in a polynucleotide encoding the parent. 
     Site-directed mutagenesis can be accomplished in vitro by PCR involving the use of oligonucleotide primers containing the desired mutation. Site-directed mutagenesis can also be performed in vitro by cassette mutagenesis involving the cleavage by a restriction enzyme at a site in the plasmid comprising a polynucleotide encoding the parent polypeptide and subsequent ligation of an oligonucleotide containing the mutation in the polynucleotide. Usually the restriction enzyme that digests the plasmid and the oligonucleotide is the same, permitting sticky ends of the plasmid and the insert to ligate to one another. See, e.g., Scherer and Davis, 1979 , Proc. Natl. Acad. Sci. USA  76: 4949-4955; and Barton et al., 1990 , Nucleic Acids Res.  18: 7349-4966. 
     Site-directed mutagenesis can also be accomplished in vivo by methods known in the art. See, e.g., U.S. Patent Application Publication No. 2004/0171154; Storici et al., 2001 , Nature Biotechnol.  19: 773-776; Kren et al., 1998 , Nat. Med.  4: 285-290; and Calissano and Macino, 1996 , Fungal Genet, Newslett.  43: 15-16. 
     Any site-directed mutagenesis procedure may be used in the present invention. There are many commercial kits available that can be used to prepare variants. The skilled person in the art is well-aware of such commercial kits and how to use them. 
     Synthetic gene construction entails in vitro synthesis of a designed polynucleotide molecule to encode a polypeptide of interest, Gene synthesis may be performed utilizing a number of techniques, such as the multiplex microchip-based technology described by Tian et al. (2004 . Nature  432: 1050-1054) and similar technologies wherein oligonucleotides are synthesized and assembled upon photo-programmable microfluidic chips. 
     Single or multiple amino acid substitutions, deletions, and/or insertions may be made and tested using known methods of mutagenesis, recombination, and/or shuffling, followed by a relevant screening procedure, such as those disclosed by Reidhaar-Olson and Sauer, 1988 , Science  241: 53-57; Bowie and Sauer, 1989 , Proc, Natl, Acad. Sci. USA  86: 2152-2156; WO 95/17413; or WO 95/22625. Other methods that may be used include error-prone FOR, phage display (e.g., Lowman et al., 1991 , Biochemistry  30: 10832-10837; U.S. Pat. No. 5,223,409; WO 92/06204) and region-directed mutagenesis (Derbyshire et al., 1986 , Gene  46: 145; Ner et al., 1988 . DNA  7: 127). 
     Mutagenesis/shuffling methods may be combined with high-throughput, automated screening methods to detect activity of cloned, mutagenized polypeptides expressed by host cells (Ness et al., 1999 , Nature Biotechnology  17: 893-896), Mutagenized DNA molecules that encode active polypeptides may be recovered from the host cells and rapidly sequenced using standard methods in the art. These methods allow the rapid determination of the importance of individual amino acid residues in a polypeptide. 
     Semi-synthetic gene construction is accomplished by combining aspects of synthetic gene construction, and/or site-directed mutagenesis, and/or random mutagenesis, and/or shuffling. Semi-synthetic construction is typified by a process utilizing polynucleotide fragments that are synthesized, in combination with PCR techniques. Defined regions of genes may thus be synthesized de novo, while other regions may be amplified using site-specific mutagenic primers, while yet other regions may be subjected to error-prone PCR or non-error prone PCR amplification. Polynucleotide subsequences may then be shuffled. 
     The present invention also relates to a method of improving wash performance of a parent polypeptide having the amino acid sequence of SEQ ID NO: 13 or 14, or having at least 80% sequence identity thereto, said method comprising the steps of:
         a) a substitution and/or deletion of two, three or four positions in the parent alpha-amylase said positions corresponding to positions 181, G182, D183, and G184 of the mature polypeptide of SEQ ID NO: 4; and   b) an alteration at one or more positions corresponding to positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14,   wherein the variant has at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 98%, at least 99%, but less than 100% sequence identity with the polypeptide of SEQ ID NO: 4 or 7, and wherein the variant has alpha-amylase activity and improved wash performance compared to the parent polypeptide.       

     In one embodiment, the variant has at least 50%, such as at least 60%, or at least 70%, or at least 80%, or at least 90%, or at least 100% of the activity of the parent polypeptide having the amino acid sequence of SEQ ID NO: 7. 
     In one embodiment, the activity is determined according to a Phadebas assay. 
     The alpha-amylase activity may be determined by a method using the Phadebas substrate (from for example Magle Life Sciences, Lund, Sweden). A Phadebas tablet includes interlinked starch polymers that are in the form of globular microspheres that are insoluble in water. A blue dye is covalently bound to these microspheres. The interlinked starch polymers in the microsphere are degraded at a speed that is proportional to the alpha-amylase activity. When the alpha-amylase degrades the starch polymers, the released blue dye is water soluble and concentration of dye can be determined by measuring absorbance at 620 nm. The concentration of blue is proportional to the alpha-amylase activity in the sample. 
     The variant sample to be analyzed is diluted in activity buffer with the desired pH. Two substrate tablets are suspended in 5 mL activity buffer and mixed on magnetic stirrer. During mixing of substrate transfer 150 μl to microtiter plate (MTP) or PCR-MTP, Add 30 μl diluted amylase sample to 150 μl substrate and mix. Incubate for 15 minutes at 37° C. The reaction is stopped by adding 30 μl 1M NaOH and mix. Centrifuge MTP for 5 minutes at 4000×g. Transfer 100 μl to new MTP and measure absorbance at 620 nm. 
     The alpha-amylase sample should be diluted so that the absorbance at 620 nm is between 0 and 2.2, and is within the linear range of the activity assay. 
     Thus, in one embodiment, the activity is determined by by a method comprising the steps of;
         a) incubating an alpha-amylase variant according to the invention with a dyed amylose substrate for 15 minute at 37° C.: and   b) measuring the absorption at OD 620 nm.       

     In a further embodiment, the activity is determined by a method comprising the steps of;
         a) incubating an alpha-amylase variant according to the invention with a dyed amylose substrate for 15 minute at 37° C.: and   b) centrifuging the sample;   c) transferring the supernatant to reader plate, and measuring the absorption at OD 620 nm,       

     Fermentation Broth Formulations or Cell Compositions 
     The present invention also relates to a fermentation broth formulation or a cell composition comprising a polypeptide of the present invention. Thus, in one embodiment, the fermentation broth formulation or the cell composition comprises a polynucleotide encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267. K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13, or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, I242, S244, G255, N260, A265, K269, S280, W284, Y295, R304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, a nucleic acid construct encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, or an expression vector encoding a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q269, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, R280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14. The fermentation broth product may further comprise additional ingredients used in the fermentation process, such as, for example, cells (including, the host cells containing the gene encoding the polypeptide of the present Invention which are used to produce the polypeptide of interest), cell debris, biomass, fermentation media and/or fermentation products. In some embodiments, the composition is a cell-Filled whole broth containing organic acid(s), killed cells and/or cell debris, and culture medium. 
     The term “fermentation broth” as used herein refers to a preparation produced by cellular fermentation that undergoes no or minimal recovery and/or purification. For example, fermentation broths are produced when microbial cultures are grown to saturation, incubated under carbon-limiting conditions to allow protein synthesis (e.g., expression of enzymes by host cells) and secretion into cell culture medium. The fermentation broth can contain unfractionated or fractionated contents of the fermentation materials derived at the end of the fermentation, Typically, the fermentation broth is unfractionated and comprises the spent culture medium and cell debris present after the microbial cells (e.g., filamentous fungal cells) are removed, e.g., by centrifugation. In some embodiments, the fermentation broth contains spent cell culture medium, extracellular enzymes, and viable and/or nonviable microbial cells. 
     In one embodiment, the fermentation broth formulation and cell compositions comprise a first organic acid component comprising at least one 1-5 carbon organic acid and/or a salt thereof and a second organic acid component comprising at least one 6 or more carbon organic acid and/or a salt thereof. In a particular embodiment, the first organic acid component is acetic acid, formic acid, propionic acid, a salt thereof, or a mixture of two or more of the foregoing and the second organic acid component is benzoic acid, cyclohexanecarboxylic acid, 4-methylvaleric acid, phenylacetic acid, a salt thereof, or a mixture of two or more of the foregoing. 
     In one embodiment, the composition contains an organic acid(s), and optionally further contains killed cells and/or cell debris. In one embodiment, the killed cells and/or cell debris are removed from a cell-killed whole broth to provide a composition that is free of these components. 
     The fermentation broth formulations or cell compositions may further comprise a preservative and/or anti-microbial (e.g., bacteriostatic) agent, including, but not limited to, sorbitol, sodium chloride, potassium sorbate, and others known in the art. 
     The cell-killed whole broth or composition may comprise the unfractionated contents of the fermentation materials derived at the end of the fermentation. Typically, the cell-killed whole broth or composition comprises the spent culture medium and cell debris present after the microbial cells (e.g., filamentous fungal cells) are grown to saturation, incubated under carbon-limiting conditions to allow protein synthesis. In some embodiments, the cell-killed whole broth or composition comprises the spent cell culture medium, extracellular enzymes, and killed filamentous fungal cells. In some embodiments, the microbial cells present in the cell-killed whole broth or composition may be permeabilized and/or lysed using methods known in the art. 
     A whole broth or cell composition as described herein is typically a liquid, but may comprise insoluble components, such as killed cells, cell debris, culture media components, and/or insoluble enzyme(s). In some embodiments, insoluble components may be removed to provide a clarified liquid composition. 
     The whole broth formulations and cell compositions of the present invention may be produced by a method described in WO 90/15861 or WO 2010/096673, 
     Enzyme Compositions 
     The present invention also relates to compositions comprising a variant according to the invention. Thus, the invention relates to a composition comprising a variant comprising an alteration in one or more positions corresponding to the positions 1-11, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476. G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14. 
     In one embodiment, the composition comprises a variant comprising
         a) a deletion and/or a substitution at two or more positions corresponding to positions R181. G182, D183, and G184 of the amino acid sequence as set forth in SEQ ID NOs; 7 or 10, and   b) an alteration at one or more positions corresponding to positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, T173, A174, A186, E190, T193, N195, A204, V206, P211, V213, I214. L217, A225, L235, V238, K242, S244, M246, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, R280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, G337, Q345, G346, T355, R376, D377, S381, Y382, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, R323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14. Preferably, the compositions are enriched in such a variant. The term “enriched” as used herein, refers to that the alpha-amylase activity of the composition has been increased, e.g., with an enrichment factor of at least 1.1.       

     The compositions may comprise a variant of the present invention as the major enzymatic component, e.g., a mono-component composition. In another embodiment, the composition further comprises at least one further active component. 
     The term “active component” as used herein, refers to any biological or non-biological molecule which in itself is active. For example, an active component is an enzyme. 
     Thus, in one embodiment, the further active component is an enzyme, such as a protease, lipase, cellulose, pectate lyase and mannanase. Thus, the compositions may comprise multiple enzymatic activities, such as one or more (e.g., several) enzymes selected from the group consisting of hydrolase, isomerase, ligase, lyase, oxidoreductase, or transferase, e.g., an alpha-galactosidase, alpha-glucosidase, aminopeptidase, amylase, beta-galactosidase, beta-glucosidase, beta-xylosidase, carbohydrase, carboxypeptidase, catalase, cellobiohydrolase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, endoglucanase, esterase, glucoamylase, invertase, laccase, lipase, mannosidase, mutanase, oxidase, pectinolytic enzyme, peroxidase, phytase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transglutaminase, or xylanase. 
     In one embodiment, the composition is a liquid laundry or liquid dish wash composition, such as an Automatic Dish Wash (ADW) liquid detergent composition, or a powder laundry, such as a soap bar, or powder dish wash composition, such as an ADW unit dose detergent composition and such as a Hand Dish Wash (HDW) detergent composition. 
     The choice of additional components is within the skills of the skilled person in the art and includes conventional ingredients, including the exemplary non-limiting components set forth below. The choice of components may include, for fabric care, the consideration of the type of fabric to be cleaned, the type and/or degree of soiling, the temperature at which cleaning is to take place, and the formulation of the detergent product. Although components mentioned below are categorized by general header according to a particular functionality, this is not to be construed as a limitation, as a component may comprise additional functionalities as will be appreciated by the skilled artisan. 
     In one embodiment of the present invention, the variant of the present invention may be added to a detergent composition in an amount corresponding to 0.001-100 mg of protein, such as 0.01-100 mg of protein, preferably 0.005-50 mg of protein, more preferably 0.01-25 mg of protein, even more preferably 0.05-10 mg of protein, most preferably 0.05-5 mg of protein, and even most preferably 0.01-1 mg of protein per liter of wash liquor. The term “protein” in this context is contemplated to be understood to include a variant according to the present invention. 
     A composition for use in automatic dish wash (ADW), for example, may include 0.0001%-50%, such as 0.001%-20%, such as 0.01%-10%, such as 0.05-5% of enzyme protein by weight of the composition. 
     A composition for use in laundry granulation, for example, may include 0.0001%-50%, such as 0.001%-20%, such as 0.01%-10%, such as 0.05%-5% of enzyme protein by weight of the composition. 
     A composition for use in laundry liquid, for example, may include 0.0001%-10%, such as 0.001-7%, such as 0.1%-5% of enzyme protein by weight of the composition. 
     The variants of the invention as well as the further active components, such as additional enzymes, may be stabilized using conventional stabilizing agents, e.g., a polyol such as propylene glycol or glycerol, a sugar or sugar alcohol, lactic acid, boric acid, or a boric acid derivative, e.g., an aromatic borate ester, or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid, and the composition may be formulated as described in, for example, WO92/19709 and WO92/19708. 
     In certain markets different wash conditions and, as such, different types of detergents are used. This is disclosed in e.g. EP 1 025 240. For example, In Asia (Japan) a low detergent concentration system is used, while the United States uses a medium detergent concentration system, and Europe uses a high detergent concentration system. 
     A low detergent concentration system includes detergents where less than about 800 ppm of detergent components are present in the wash water. Japanese detergents are typically considered low detergent concentration system as they have approximately 667 ppm of detergent components present in the wash water. 
     A medium detergent concentration includes detergents where between about 800 ppm and about 2000 ppm of detergent components are present in the wash water. North American detergents are generally considered to be medium detergent concentration systems as they have approximately 975 ppm of detergent components present in the wash water. 
     A high detergent concentration system includes detergents where greater than about 2000 ppm of detergent components are present in the wash water. European detergents are generally considered to be high detergent concentration systems as they have approximately 4500-5000 ppm of detergent components in the wash water. 
     Latin American detergents are generally high suds phosphate builder detergents and the range of detergents used in Latin America can fall in both the medium and high detergent concentrations as they range from 1500 ppm to 6000 ppm of detergent components in the wash water. Such detergent compositions are all embodiments of the invention. 
     A variant of the present invention may also be incorporated in the detergent formulations disclosed in WO97/07202, which is hereby incorporated by reference. 
     Examples are given herein of preferred uses of the compositions of the present invention. The dosage of the composition and other conditions under which the composition is used may be determined on the basis of methods known in the art. 
     In particular, a composition according to the present invention further comprises a chelator. 
     The term “chelator” as used herein, refers to chemicals which form molecules with certain metal ions, inactivating the ions so that they cannot react with other elements. Thus, a chelator may be defined as a binding agent that suppresses chemical activity by forming chelates. 
     Chelation is the formation or presence of two or more separate bindings between a ligand and a single central atom. The ligand may be any organic compound, a silicate or a phosphate. In the present context the term “chelating agents” comprises chelants, chelating agent, chelating agents, complexing agents, or sequestering agents that forms water-soluble complexes with metal ions such as calcium and magnesium. The chelate effect describes the enhanced affinity of chelating ligands for a metal ion compared to the affinity of a collection of similar nonchelating ligands for the same metal. Chelating agents having binding capacity with metal ions, in particular calcium (Ca2+) ions, and has been used widely in detergents and compositions in general for wash, such as laundry or dish wash. Chelating agents have however shown themselves to inhibit enzymatic activity. The term chelating agent is used in the present application interchangeably with “complexing agent” or “chelating agent” or “chelant”. 
     Since most alpha-amylases are calcium sensitive the presence of chelating agents these may impair the enzyme activity. The calcium sensitivity of alpha-amylases can be determined by incubating a given alpha-amylase in the presence of a strong chelating agent and analyze the impact of this incubation on the activity of the alpha-amylase in question. A calcium sensitive alpha-amylase will lose a major part or all of its activity during the incubation. Chelating agent may be present in the composition in an amount from 0.0001 wt % to 20 wt %, preferably from 0.01 to 10 wt %, more preferably from 0.1 to 5 wt %. 
     Non-limiting examples of chelating agents are; EDTA, DTMPA, HEDP, and citrate. Thus, in one embodiment, the composition comprises a variant according to the invention and a chelating agent, such as EDTA, DTMPA, HEDP or citrate. 
     The term “EDTA” as used herein, refers to ethylene-diamine-tetra-acetic acid which falls under the definition of “strong chelating agents”. 
     The term “DTMPA” as used herein, refers to diethylenetriamine penta(methylene phosphonic acid). DTMPA can inhibit the scale formation of carbonate, sulfate and phosphate. 
     The term “HEDP” as used herein, refers to hydroxy-ethane diphosphonic acid, which falls under the definition of “strong chelating agents”. 
     The chelate effect or the chelating effect describes the enhanced affinity of chelating ligands for a metal ion compared to the affinity of a collection of similar nonchelating ligands for the same metal. However, the strength of this chelate effect can be determined by various types of assays or measure methods thereby differentiating or ranking the chelating agents according to their chelating effect (or strength). 
     In an assay the chelating agents may be characterized by their ability to reduce the concentration of free calcium ions (Ca2+) from 2.0 mM to 0.10 mM or less at pH 8.0, e.g. by using a test based on the method described by M. K. Nagarajan et al., JAOCS, Vol. 61, no. 9 (September 1984), pp. 1475-1478. 
     For reference, a chelator having the same ability to reduce the concentration of free calcium ions (Ca2+) from 2.0 mM to 0.10 mM at pH as EDTA at equal concentrations of the chelator are said to be strong chelators. 
     The composition of the present invention may be in any convenient form, e.g., a bar, a homogenous tablet, a tablet having two or more layers, a pouch having one or more compartments, a regular or compact powder, a granule, a paste, a gel, or a regular, compact or concentrated liquid. There are a number of detergent formulation forms such as layers (same or different phases), pouches, as well as forms for machine dosing unit. 
     Pouches can be configured as single or multicompartments. It can be of any form, shape and material which is suitable for hold the composition, e.g. without allowing the release of the composition from the pouch prior to water contact. The pouch is made from water soluble film which encloses an inner volume. Said inner volume can be divided into compartments of the pouch. Preferred films are polymeric materials preferably polymers which are formed into a film or sheet. Preferred polymers, copolymers or derivatives thereof are selected polyacrylates, and water soluble acrylate copolymers, methyl cellulose, carboxy methyl cellulose, sodium dextrin, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, malto dextrin, poly methacrylates, most preferably polyvinyl alcohol copolymers and, hydroxypropyl methyl cellulose (HPMC). Preferably the level of polymer in the film for example PVA is at least about 60%. Preferred average molecular weight will typically be about 20,000 to about 150,000. Films can also be of blend compositions comprising hydrolytically degradable and water soluble polymer blends such as polyactide and polyvinyl alcohol (known under the Trade reference M8630 as sold by Chris Craft In. Prod, Of Gary, Ind., US) plus plasticisers like glycerol, ethylene glycerol, Propylene glycol, sorbitol and mixtures thereof. The pouches can comprise a solid laundry cleaning composition or part components and/or a liquid cleaning composition or part components separated by the water soluble film. The compartment for liquid components can be different in composition than compartments containing solids. Ref: (US2009/0011970 A1). 
     Detergent ingredients may be separated physically from each other by compartments in water dissolvable pouches or in different layers of tablets. Thereby negative storage interaction between components may be avoided. Different dissolution profiles of each of the compartments can also give rise to delayed dissolution of selected components in the wash solution. 
     A liquid or gel detergent, which is not unit dosed, may be aqueous, typically containing at least 20% by weight and up to 95% water, such as up to about 70% water, up to about 65% water, up to about 55% water, up to about 45% water, up to about 35% water. Other types of liquids, including without limitation, alkanols, amines, dials, ethers and polyols may be included in an aqueous liquid or gel. An aqueous liquid or gel detergent may contain from 0-30% organic solvent. A liquid or gel detergent may be non-aqueous. 
     Another form of composition is in the form of a soap bar, such as a laundry soap bar, and may be used for hand washing laundry, fabrics and/or textiles. The term “soap bar” as used herein, refers to includes laundry bars, soap bars, combo bars, syndet bars and detergent bars. The types of bar usually differ in the type of surfactant they contain, and the term laundry soap bar includes those containing soaps from fatty acids and/or synthetic soaps. The laundry soap bar has a physical form which is solid and not a liquid, gel or a powder at room temperature. The term “solid” as used herein, refers to a physical form which does not significantly change over time, i.e. if a solid object (e.g. laundry soap bar) is placed inside a container, the solid object does not change to fill the container it is placed in. The bar is a solid typically in bar form but can be in other solid shapes such as round or oval. 
     The soap bar may also comprise complexing agents like EDTA and HEDP, perfumes and/or different type of fillers, surfactants e.g. anionic synthetic surfactants, builders, polymeric soil release agents, detergent chelators, stabilizing agents, fillers, dyes, colorants, dye transfer inhibitors, alkoxylated polycarbonates, suds suppressers, structurants, binders, leaching agents, bleaching activators, clay soil removal agents, anti-redeposition agents, polymeric dispersing agents, brighteners, fabric softeners, perfumes and/or other compounds known in the art. 
     The soap bar may be processed in conventional laundry soap bar making equipment such as but not limited to: mixers, plodders, e.g. a two stage vacuum plodder, extruders, cutters, logo-stampers, cooling tunnels and wrappers. The invention is not limited to preparing the soap bars by any single method. The premix of the invention may be added to the soap at different stages of the process. For example, the premix comprising a soap, an enzyme, optionally one or more additional enzymes, a protease inhibitor, and a salt of a monovalent cation and an organic anion may be prepared and the mixture may then plodded. The enzyme and optional additional enzymes may be added at the same time as an enzyme inhibitor, e.g. a protease inhibitor, for example in liquid form. Besides the mixing step and the plodding step, the process may further comprise the steps of milling, extruding, cutting, stamping, cooling and/or wrapping. 
     Uses 
     The present invention further relates to the use of a variant according to the present invention in a cleaning process such as laundry or hard surface cleaning including automated dish wash and industrial cleaning. The soils and stains that are important for cleaning are composed of many different substances, and a range of different enzymes, all with different substrate specificities, have been developed for use in detergents both in relation to laundry and hard surface cleaning, such as dishwashing. These enzymes are considered to provide an enzyme detergency benefit, since they specifically improve stain removal in the cleaning process that they are used in, compared to the same process without enzymes. Stain removing enzymes that are known in the art include enzymes such as proteases, amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases, catalases and mannanases. 
     In one embodiment, the invention relates the use of variants of the present invention in detergent compositions, for use in cleaning hard-surfaces, such as dish wash, or in laundering or for stain removal. In another embodiment, the invention relates to the use of an alpha-amylase variant according to the invention in a cleaning process such as laundry or hard surface cleaning including, but not limited to, dish wash and industrial cleaning. Thus, in one embodiment, the invention relates to the use of a variant comprising an alteration in one or more positions corresponding to the positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14 in a cleaning process such as laundry or hard surface cleaning including dish wash and industrial cleaning. 
     In a particular embodiment, the invention relates to the use of a variant comprising a) a substitution and/or deletion of two, three or four positions in the parent alpha-amylase said positions corresponding to positions 181, G182, D183, and G184 of the mature polypeptide of SEQ ID NO: 4; and
         b) an alteration at one or more positions corresponding to positions positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, Y295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13: or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, Y295, S304, R320, H321, S323, V326, K391, I405, A421, A422, A428, R439, G448, W467, D476, and G477 of SEQ ID NO: 14, in a cleaning process such as laundry or hard surface cleaning including dish wash and industrial cleaning.       

     In one embodiment of the invention relates the use of a composition according to the invention comprising a variant of the present invention together with one or more surfactants and optionally one or more detergent components, selected from the list comprising of hydrotropes, builders and co-builders, bleaching systems, polymers, fabric hoeing agents and adjunct materials, or any mixture thereof in detergent compositions and in detergent applications. 
     A further embodiment is the use of the composition according to the invention comprising a variant of the present invention together with one or more surfactants, and one or more additional enzymes selected from the group comprising of proteases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases, catalases and mannanases, or any mixture thereof in detergent compositions and in detergent applications. 
     In another aspect, the invention relates to a laundering process which may be for household laundering as well as industrial laundering. Furthermore, the invention relates to a process for the laundering of textiles (e.g. fabrics, garments, cloths etc.) where the process comprises treating the textile with a washing solution containing a detergent composition and an alpha-amylase of the present invention. The laundering can for example be carried out using a household or an industrial washing machine or be carried out by hand using a detergent composition containing a glucoamylase of the invention. 
     In another aspect, the invention relates to a dish wash process which may be for household dish wash as well as industrial dish wash. The term “dish wash” as used herein, refers to both manual dish wash and automated dish wash. Furthermore, the invention relates to a process for the washing of hard surfaces (e.g. cutlery such as knives, forks, spoons; crockery such as plates, glasses, bowls; and pans) where the process comprises treating the hard surface with a washing solution containing a detergent composition and an alpha-amylase variant of the present invention. The hard surface washing can for example be carried out using a household or an industrial dishwasher or be carried out by hand using a detergent composition containing an alpha-amylase of the invention, optionally together with one or more further enzymes selected from the group comprising of proteases, amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases, catalases, mannanases, or any mixture thereof. 
     In a further aspect, the invention relates to a method for removing a stain from a surface comprising contacting the surface with a composition comprising an alpha-amylase of the present invention together with one or more surfactants and optionally one or more detergent components selected from the list comprising of hydrotropes, builders and co-builders, bleaching systems, polymers, fabric hoeing agents and adjunct materials, or any mixture thereof in detergent compositions and in detergent applications. A further aspect is a method for removing a stain from a surface comprising contacting the surface with a composition comprising an alpha-amylase variant of the present invention together with one or more surfactants, one or more additional enzymes selected from the group comprising of proteases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases, catalases and mannanases, or any mixture thereof in detergent compositions and in detergent applications. 
     The present invention is further described by the following examples that should not be construed as limiting the scope of the invention. 
     EXAMPLES 
     Example 1a: Construction of the Parent Polypeptide as Set Forth in SEQ ID NO: 13 
     Construction of a hybrid between the A and B domain from a polypeptide having the amino acid sequence set forth SEQ ID NO: 10 (the first parent polypeptide) and the C domain from a polypeptide having the amino acid sequence set forth SEQ ID NO: 11 (the second parent polypeptide). 
     Based on 3D structural alignment of the two amylases, amino acid no 1 to amino acid no 397 of the first parent polypeptide are defined as domain A and B, and amino acid 398 to amino acid no. 485 of the second parent polypeptide are defined as the C domain. Synthetic DNA fragments coding for part of the A domain from the first parent polypeptide and the C domain from the second parent polypeptide were designed and purchased from an external vendor. In addition to combining fragments of the two different amylases, the following stabilizing substitutions were designed into the synthetic amylase gene: G182* and D183*. 
     The new gene consisting of a gene fragment encoding the A and B domain of the first parent polypeptide and the C domain of the second parent polypeptide coded by the synthetic gene fragments was constructed by triple SOE (splicing by overlap extension) PCR method. The two fragments were assembled by splicing by overlap extension polymerase chain reaction and transformed into a competent  B. subtilis  host for overexpression. The resulting amylase consisting of the A and B domain from the first parent polypeptide and the C domain from the second parent polypeptide, and having the deletions of G182* and D183* is the polypeptide as set forth in SEQ ID NO: 13. 
     Example 1b: Construction of the Parent Polypeptide as Set Forth in SEQ ID NO: 14 
     Construction of a hybrid between the A and B domain from a polypeptide having the amino acid sequence set forth SEQ ID NO: 10 (the first parent polypeptide) and the C domain from a polypeptide having the amino acid sequence set forth SEQ ID NO: 12 (the third parent polypeptide). 
     Based on 3D structural alignment of the two amylases, amino acid no 1 to amino acid no 397 of the first parent polypeptide are defined as domain A and B, and amino acid 398 to amino acid no. 485 of the third parent polypeptide are defined as the C domain. Synthetic DNA fragments coding for part of the A domain from the first parent polypeptide and the C domain from the second parent polypeptide were designed and purchased from an external vendor. In addition to combining fragments of the two different amylases, the following stabilizing substitutions were designed into the synthetic amylase gene: G182* and D183*. 
     The new gene consisting of a gene fragment encoding the A and B domain of the first parent polypeptide and the C domain of the second parent polypeptide coded by the synthetic gene fragments was constructed by triple SOE (splicing by overlap extension) PCR method. A DNA fragment was amplified from an expression clone of the first amylase. The fragments were finally assembled by triple SOE and the derived PCR fragment was transformed into a suitable  B. subtilis  host and the gene integrated into the  B. subtilis  chromosome by homologous recombination into the pectate lyase (pel) locus. The gene coding for chloramphenicol acetyltransferase was used as maker (as described in (Diderichsen et al., 1993, Plasmid 30: 312-315), chloramphenicol resistant clones were analyzed by DNA sequencing to verify the correct DNA sequence of the construct. The resulting amylase consisting of the A and B domain from the first parent polypeptide and the C domain from the second parent polypeptide, and having the deletions of G182* and D183* is the polypeptide as set forth in SEQ ID NO: 14. 
     Example 2: Generation of Variants According to the Invention 
     The variants of the present invention have been generated by site-directed mutagenesis. Genomic DNA prepared from the organism containing amylase gene at the Pel locus was used as template for generating the site-directed mutants. 
     Mutagenic forward primer and PnMi4490 (CAATCCAAGAGAACCCTGATACGGATG—SEQ ID NO: 16) reverse primer was used to generate a ˜3.8 kb fragment. This fragment was used as a megaprimer along with PnMi4491 (CGGAACGCCTGGCTGACAACACG—SEQ ID NO: 17) forward primer to get 6 kb insertion cassette. To enable integration in the Pel locus by double cross-over upon transformation, along with the amylase and cat genes, the cassette contained upstream and downstream Pei sequences at the ends, Selection was done on LB Agar containing chloramphenicol and the mutation was confirmed by DNA sequencing of amylase gene. 
     A library of variants were generated where different combinations of alterations in the following positions were made: H1, T5, G7. Q11, N16, V17, Q32, A37, I40, P45, W48, G50, T51, N54, V56, A60, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, G196, A204, V206, P211, V213, I214, L217, A225, L228, L235, V238, K242, S244, M246, L2502, G255, N260, A263, V264, A265, Y267, K269. N270, G273, R280, W284, T285, M286, F289, V291, Y295, Q299, S304, R320, H321, S323, H324, V326, F328, T334, G337, Q345, G346, T355, R376, D377, D381. Y382, Q385, K391, and Q395 of SEQ ID NOs: 7 or 10: A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO: 13; and I405, A421, A422, A428, G448, D476, and G477 of SEQ ID NO: 14. 
     The generated variants are listed in the following examples. 
     Example 3: Wash Performance of Generated Variants Using Automatic Mechanical Stress Assay (AMSA) 
     In order to assess the wash performance of the variants of the present invention in a detergent base composition, washing experiments may be performed using Automatic Mechanical Stress Assay (AMSA). With the AMSA test the wash performance of a large quantity of small volume enzyme-detergent solutions can be examined. The AMSA plate has a number of slots for test solutions and a lid firmly squeezing the textile swatch to be washed against all the slot openings. During the washing time, the plate, test solutions, textile and lid were vigorously shaken to bring the test solution in contact with the textile and apply mechanical stress in a regular, periodic oscillating manner. For further description see WO 02/42740, especially the paragraph “Special method embodiments” at page 23-24. 
     General Wash Performance Description 
     A test solution comprising water (6° dH), 0.79 g/L detergent, e.g. Model detergent J as described below, and the enzyme of the invention at concentration of 0, 0.05 or 0.2 mg enzyme protein/L, is prepared. Fabrics stained with starch (CS-28 from Center For Test materials BY, P.O. Box 120, 3133 KT, Vlaardingen, The Netherlands) was added and washed for 20 minutes at 15° C. and 30° C., or alternatively 20 minutes at 15° C. and 40° C. as specified below. After thorough rinse under running tap water and drying in the dark, the light intensity values of the stained fabrics were subsequently measured as a measure for wash performance. The test with 0 mg enzyme protein/L was used as a blank and corresponded to the contribution from the detergent. Preferably mechanical action is applied during the wash step, e.g. in the form of shaking, rotating or stirring the wash solution with the fabrics. The AMSA wash performance experiments was conducted under the experimental conditions specified below: 
     
       
         
           
               
             
               
                 TABLE A 
               
               
                   
               
               
                 Experimental condition 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Detergent 
                 Liquid Model detergent J (see Table B) 
               
               
                 Detergent dosage 
                 0.79 g/L 
               
               
                 Test solution volume 
                 160 micro L 
               
               
                 pH 
                 As is 
               
               
                 Wash time 
                 20 minutes 
               
               
                 Temperature 
                 15° C. or 30° C. 
               
               
                 Water hardness 
                 6° dH 
               
               
                 Enzyme concentration 
                 0.2 mg enzyme protein/L and 0.05 mg enzyme 
               
               
                 in test 
                 protein/L 
               
               
                 Test material 
                 CS-28 (Rice starch cotton) 
               
               
                   
               
            
           
         
       
     
                     TABLE B                  Model detergent J                             Content of compound   % active component       Compound   (% w/w)   (% w/w)                                 LAS   5.15   5.00       linear alkylbenzene       sulfonates       AS   5.00   4.50       alkylbenzene sulfonates       AEOS   14.18   10.00       alkyl ethoxy sulfate       Coco fatty acid   1.00   1.00       AEO   5.00   5.00       alkyl ethoxylate       MEA   0.30   0.30       monoethanolamine       MPG   3.00   3.00       monopropylene glycol       Ethanol   1.50   1.35       DTPA (as Na5 salt)   0.25   0.10       pentasodium       diethylenetriaminepenta-       acetic acid       Sodium citrate   4.00   4.00       Sodium formate   1.00   1.00       Sodium hydroxide   0.66   0.66       H 2 O, ion exchanged   58.95   58.95                    
Water hardness was adjusted to 6° dH by addition of CaCl 2 ), MgCi 2 , and NaHCO 2 , (Ca 2+ :Mg 2+ :HCO 3+ =2:1:4.5) to the test system. After washing the textiles were flushed in tap water and dried.
 
     
       
         
           
               
             
               
                 TABLE C 
               
               
                   
               
               
                 Experimental condition 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Detergent 
                 Liquid Model detergent A (see Table D) 
               
               
                 Detergent dosage 
                 3.33 g/L 
               
               
                 Test solution volume 
                 160 micro L 
               
               
                 pH 
                 As is 
               
               
                 Wash time 
                 20 minutes 
               
               
                 Temperature 
                 15° C. or 40° C. 
               
               
                 Water hardness 
                 15° dH 
               
               
                 Enzyme concentration 
                 0.2 mg enzyme protein/L, 0.05 mg enzyme 
               
               
                 in test 
                 protein/L 
               
               
                 Test material 
                 CS-28 (Rice starch cotton) 
               
               
                   
               
            
           
         
       
     
                     TABLE D                  Model detergent A                             Content of compound   % active component       Compound   (% w/w)   (% w/w)                                 LAS   12.00   11.60       (linear alkylbenzene       sulfonates)       AEOS   17.63   4.90       (alkyl ethoxy sulfate),       SLES       (sodium lauryl ether       sulfate)       Soy fatty acid   2.75   2.48       Coco fatty acid   2.75   2.80       AEO (alkyl ethoxylate)   11.00   11.00       Sodium hydroxide   1.75   1.80       Ethanol/Propan-2-ol   3.00   2.70/0.30       MPG   6.00   6.00       monopropylene glycol       Glycerol   1.71   1.70       TEA (triethanolamine)   3.33   3.30       Sodium formate   1.00   1.00       Sodium citrate   2.00   2.00       DTMPA   0.48   0.20       (diethylenetriaminepenta-       acetic acid)       PCA   0.46   0.18       polycarboxylic acid type       polymer       Phenoxy ethanol   0.50   0.50       H 2 O, ion exchanged   33.64   33.64                    
Water hardness was adjusted to 15° dH by addition of CaCl 2 ), MgCl 2 , and NaHCO 3  (Ca 2+ :Mg 2+ :HCO 3 =4:1:7.5) to the test system. After washing the textiles were flushed in tap water and dried.
 
     The wash performance was measured as the brightness expressed as the intensity of the light reflected from the sample when illuminated with white light. When the sample was stained the intensity of the reflected light was lower, than that of a clean sample. Therefore the intensity of the reflected light can be used to measure wash performance. 
     Color measurements were made with a professional flatbed scanner (EPSON Expression 10000XL, EPSON) used to capture an image of the washed textile. 
     To extract a value for the light intensity from the scanned images, 48→24 Bit Color pixel values from the image were converted into values for red, green and blue (RGB). The intensity value (Int) is calculated by adding the RGB values together as vectors and then taking the length of the resulting vector: 
     
       
         
           
             Int 
             = 
             
               
                 
                   r 
                   2 
                 
                 + 
                 
                   g 
                   2 
                 
                 + 
                 
                   b 
                   2 
                 
               
             
           
         
       
     
     The term “improved wash performance” of the present experiment is defined as displaying an alteration of the wash performance of a variant of the present invention relative to the wash performance of the polypeptide having an amino acid sequence as set forth in SEQ ID NO: 15, The alteration may e.g. be seen as increased stain removal. Improved wash performance was determined as described above. The wash performance was considered to be improved if the Improvement Factor (IF) is at least 1.0, preferably at least 1.05 in one or more of the conditions listed above; i.e. either in Model detergent A at 15° C. or 40° C., where the variant concentration was 0.05 or 0.2 mg/L or in Model detergent J at 15° C. or 30° C., where the variant concentration was 0.05 or 0.2 mg/L. The wash conditions were as described above in Tables A and C. 
     The terms “Delta intensity” or “Delta intensity value” as used herein refers to the result of an intensity measurement of a test material, e.g. a swatch CS-28 (Center For Testmaterials BY, P.O. Box 120, 3133 KT Vlaardingen, the Netherlands)—. The swatch was measured with a portion of the swatch, washed under identical conditions, as background. The delta intensity is the intensity value of the test material washed with amylase subtracting the intensity value of the test material washed without amylase. 
     The wash performance of the variants according to the invention obtained by AMSA are the following; 
     
       
         
           
               
               
               
               
               
               
               
               
               
             
               
                   
               
             
            
               
                   
                 A-15- 
                 A-15- 
                 A-40- 
                 A-40- 
                 J-15- 
                 J-15- 
                 J-30- 
                 J-30- 
               
               
                   
                 0.05 
                 0.2 
                 0.05 
                 0.2 
                 0.05 
                 0.2 
                 0.05 
                 0.2 
               
               
                 Modifications in SEQ ID NO: 13 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
               
               
                   
               
               
                 Reference 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
               
               
                 G182* + D183* + N195F 
                 1.0 
                 1.3 
                 1.1 
                 1.0 
                 1.5 
                 1.3 
                 1.0 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.9 
                 1.2 
                 1.2 
                 2.6 
                 2.0 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + R87S + G109A + A174S + G182* + 
                 2.8 
                 2.2 
                 1.3 
                 1.1 
                 2.6 
                 2.4 
                 1.9 
                 1.5 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T40G + N54S + V56T + G109A + A174S + G182* + 
                 2.2 
                 1.7 
                 1.3 
                 1.1 
                 2.5 
                 2.2 
                 1.9 
                 1.6 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51K + N54S + V56T + G109A + A174S + G182* + 
                 2.7 
                 2.1 
                 1.3 
                 1.1 
                 3.2 
                 2.3 
                 2.0 
                 1.5 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 2.6 
                 2.2 
                 1.2 
                 1.0 
                 2.6 
                 2.5 
                 1.7 
                 1.5 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72H + G109A + A174S + G182* + 
                 2.5 
                 2.4 
                 1.4 
                 1.1 
                 2.6 
                 2.3 
                 1.7 
                 1.6 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + A37H + N54S + V56T + G109A + A174S + G182* + 
                 2.8 
                 2.7 
                 1.4 
                 1.2 
                 2.8 
                 2.8 
                 1.9 
                 1.6 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + A37M + N54S + V56T + G109A + A174S + G182* + 
                 2.0 
                 2.3 
                 1.4 
                 1.2 
                 2.7 
                 2.5 
                 2.1 
                 1.6 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + A37V + N54S + V56T + G109A + A174S + G182* + 
                 3.0 
                 2.6 
                 1.4 
                 1.2 
                 3.1 
                 2.5 
                 2.2 
                 1.7 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + A37S + N54S + V56T + G109A + A174S + G182* + 
                 2.9 
                 2.5 
                 1.2 
                 1.2 
                 2.9 
                 2.3 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + A37Y + N54S + V56T + G109A + A174S + G182* + 
                 2.6 
                 2.0 
                 1.3 
                 1.2 
                 2.5 
                 2.1 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + A37R + N54S + V56T + G109A + A174S + G182* + 
                 2.2 
                 1.9 
                 1.3 
                 1.2 
                 2.5 
                 2.1 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113W + A174S + G182* + 
                 2.8 
                 2.2 
                 1.3 
                 1.2 
                 2.7 
                 2.1 
                 1.5 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113S + A174S + G182* + 
                 2.3 
                 1.9 
                 1.3 
                 1.1 
                 2.4 
                 1.7 
                 1.3 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113N + A174S + G182* + 
                 2.0 
                 1.9 
                 1.2 
                 1.2 
                 2.3 
                 2.1 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Y + A174S + G182* + 
                 1.9 
                 2.1 
                 1.2 
                 1.2 
                 3.1 
                 2.3 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113R + A174S + G182* + 
                 1.0 
                 1.4 
                 1.2 
                 1.2 
                 2.0 
                 1.8 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118N + A174S + G182* + 
                 1.9 
                 1.8 
                 1.3 
                 1.2 
                 2.9 
                 2.3 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116Q + A174S + G182* + 
                 2.6 
                 2.0 
                 1.2 
                 1.2 
                 3.5 
                 2.6 
                 1.7 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116V + A174S + G182* + 
                 2.1 
                 2.0 
                 1.1 
                 1.1 
                 2.7 
                 2.3 
                 1.6 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116K + A174S + G182* + 
                 2.2 
                 2.1 
                 1.2 
                 1.1 
                 2.4 
                 2.2 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116W + A174S + G182* + 
                 1.8 
                 2.0 
                 0.7 
                 0.9 
                 1.1 
                 1.9 
                 1.0 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116L + A174S + G182* + 
                 1.8 
                 1.8 
                 1.2 
                 1.1 
                 1.9 
                 2.0 
                 1.3 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + G142T + A174S + G182* + 
                 1.6 
                 1.7 
                 1.2 
                 1.1 
                 1.9 
                 2.0 
                 1.3 
                 1.1 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q125P + A174S + G182* + 
                 2.1 
                 2.1 
                 1.2 
                 1.1 
                 2.4 
                 2.0 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q125K + A174S + G182* + 
                 1.9 
                 1.8 
                 1.3 
                 1.1 
                 2.6 
                 1.9 
                 1.5 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 2.1 
                 1.0 
                 1.0 
                 2.5 
                 2.2 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + S381G + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.1 
                 1.0 
                 2.6 
                 1.8 
                 1.5 
                 1.2 
               
               
                 N195F + V206L + M246T + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.9 
                 1.1 
                 1.1 
                 2.8 
                 2.1 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + V213T + K391A + G476K 
               
               
                 H1* + A37L + N54S + V56T + G109A + A174S + G182* + 
                 2.5 
                 2.2 
                 1.2 
                 1.1 
                 2.5 
                 2.2 
                 1.8 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118K + A174S + G182* + 
                 1.8 
                 2.0 
                 1.2 
                 1.1 
                 1.5 
                 1.6 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116A + A174S + G182* + 
                 2.7 
                 2.3 
                 1.2 
                 1.1 
                 2.4 
                 2.1 
                 1.6 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116H + A174S + G182* + 
                 2.9 
                 2.4 
                 1.3 
                 1.2 
                 2.4 
                 2.3 
                 1.9 
                 1.5 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q125A + A174S + G182* + 
                 2.1 
                 2.0 
                 1.1 
                 1.2 
                 3.1 
                 2.1 
                 1.5 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172G + A174S + G182* + 
                 2.6 
                 2.1 
                 1.4 
                 1.2 
                 2.7 
                 2.2 
                 2.1 
                 1.5 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172R + A174S + G182* + 
                 2.6 
                 2.2 
                 1.4 
                 1.2 
                 2.7 
                 2.4 
                 2.0 
                 1.5 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.4 
                 1.7 
                 1.3 
                 1.2 
                 2.8 
                 1.8 
                 1.7 
                 1.3 
               
               
                 N195F + V206L + D377S + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.7 
                 1.2 
                 1.1 
                 2.3 
                 1.5 
                 1.7 
                 1.3 
               
               
                 N195F + V206L + D377G + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.5 
                 1.1 
                 1.1 
                 2.6 
                 1.6 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + D377R + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.2 
                 1.0 
                 2.8 
                 1.9 
                 1.5 
                 1.2 
               
               
                 N195F + V206L + D377A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.6 
                 1.3 
                 1.1 
                 2.9 
                 1.7 
                 1.5 
                 1.1 
               
               
                 N195F + V206L + I214G + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 1.6 
                 1.4 
                 1.2 
                 2.8 
                 1.9 
                 1.6 
                 1.4 
               
               
                 N195F + V206L + S381A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167I + A174S + G182* + 
                 1.7 
                 1.9 
                 1.3 
                 1.2 
                 2.8 
                 1.8 
                 1.6 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167G + A174S + G182* + 
                 1.5 
                 1.8 
                 1.2 
                 1.1 
                 2.5 
                 1.8 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.8 
                 1.3 
                 1.2 
                 2.8 
                 2.0 
                 1.6 
                 1.3 
               
               
                 N195F + V206L + G346S + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.9 
                 1.3 
                 1.1 
                 2.9 
                 2.1 
                 1.6 
                 1.3 
               
               
                 N195F + V206L + G346P + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.7 
                 1.3 
                 1.1 
                 2.2 
                 2.1 
                 1.7 
                 1.3 
               
               
                 N195F + V206L + I214H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 1.8 
                 1.3 
                 1.1 
                 2.9 
                 2.0 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + I214S + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.7 
                 1.3 
                 1.2 
                 2.4 
                 1.7 
                 1.6 
                 1.3 
               
               
                 N195F + V206L + I214T + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.7 
                 1.2 
                 1.1 
                 1.9 
                 1.7 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + K391A + A420Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.8 
                 1.2 
                 1.1 
                 2.6 
                 1.8 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + K391A + A420S + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.9 
                 1.3 
                 1.2 
                 2.3 
                 1.8 
                 1.7 
                 1.4 
               
               
                 N195F + V206L + K391A + A420K + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 2.0 
                 1.3 
                 1.2 
                 2.4 
                 1.9 
                 1.2 
                 1.3 
               
               
                 N195F + V206L + K391A + A420L + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.3 
                 1.2 
                 2.9 
                 1.9 
                 1.1 
                 1.3 
               
               
                 N195F + V206L + K391A + K391Y + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.9 
                 1.3 
                 1.2 
                 2.5 
                 1.7 
                 1.4 
                 1.4 
               
               
                 N195F + V206L + K391A + K391R + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.9 
                 1.2 
                 1.2 
                 3.4 
                 2.0 
                 2.1 
                 1.3 
               
               
                 N195F + V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.5 
                 1.8 
                 1.5 
                 1.1 
                 3.4 
                 1.9 
                 1.9 
                 1.4 
               
               
                 N195F + V206L + K391A + P473A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.5 
                 1.9 
                 1.3 
                 1.2 
                 2.8 
                 1.9 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + K391A + P473G + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.7 
                 1.3 
                 1.2 
                 2.5 
                 1.9 
                 1.5 
                 1.2 
               
               
                 N195F + V206L + K391A + P473T + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.6 
                 1.3 
                 1.1 
                 2.3 
                 1.8 
                 1.8 
                 1.4 
               
               
                 N195F + V206L + K391A + Q395P + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.8 
                 1.3 
                 1.2 
                 2.8 
                 2.0 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + K391A + T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.8 
                 1.3 
                 1.2 
                 2.8 
                 2.0 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + K391A + T444S + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 1.8 
                 1.3 
                 1.3 
                 3.0 
                 2.3 
                 1.7 
                 1.4 
               
               
                 N195F + V206L + K391A + T444D + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.7 
                 1.5 
                 1.2 
                 2.0 
                 2.1 
                 1.8 
                 1.5 
               
               
                 N195F + V206L + K391A + T444Y + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 1.7 
                 1.3 
                 1.1 
                 2.5 
                 2.0 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + S280W + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.7 
                 1.2 
                 1.1 
                 2.5 
                 1.8 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + S280L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.6 
                 1.1 
                 1.1 
                 2.4 
                 1.6 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + S280T + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.4 
                 1.2 
                 1.0 
                 2.3 
                 1.8 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + S280A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.7 
                 1.2 
                 1.2 
                 2.6 
                 2.1 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + G255N + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.8 
                 1.3 
                 1.2 
                 2.3 
                 2.2 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + T285L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.7 
                 1.2 
                 1.2 
                 2.2 
                 2.1 
                 1.6 
                 1.3 
               
               
                 N195F + V206L + T285Q + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.6 
                 1.1 
                 1.1 
                 1.8 
                 1.6 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + K391A + Q449T + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.7 
                 1.3 
                 1.1 
                 2.2 
                 2.0 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + P473K + G476K 
               
               
                 H1* + N54S + V56T + K72S + G109A + A174S + G182* + 
                 2.5 
                 2.3 
                 1.2 
                 1.1 
                 3.7 
                 2.2 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T40K + N54S + V56T + G109A + A174S + G182* + 
                 2.3 
                 2.1 
                 1.2 
                 1.1 
                 2.0 
                 1.7 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + G346T + K391A + G476K 
               
               
                 H1* + G50A + N54S + V56T + G109A ++ Q172N + 
                 2.4 
                 2.1 
                 1.2 
                 1.2 
                 2.0 
                 1.9 
                 1.4 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.7 
                 1.3 
                 1.0 
                 2.4 
                 1.6 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + K391A + G476K + K484A 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.8 
                 1.2 
                 1.0 
                 2.2 
                 1.8 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + K391A + G476K + K484G 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.7 
                 1.1 
                 1.0 
                 2.4 
                 2.0 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + K391A + G476K + K484P 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.4 
                 1.2 
                 1.1 
                 2.5 
                 1.7 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + G476K + K484E 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.5 
                 1.1 
                 1.0 
                 2.0 
                 1.3 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + G476K + K484Q 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 2.0 
                 1.2 
                 1.2 
                 2.7 
                 1.7 
                 1.6 
                 1.4 
               
               
                 N195F + V206L + K391A + G476K + K484S 
               
               
                 H1*K + G50A + N54S + V56T + G109A + W167F + A174S + 
                 2.1 
                 1.9 
                 1.1 
                 1.1 
                 2.2 
                 1.7 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + L173V + A174S + G182* + 
                 2.2 
                 1.8 
                 1.1 
                 1.1 
                 2.2 
                 1.7 
                 1.1 
                 1.1 
               
               
                 D183* + N195F + A204T + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W140Y + A174S + G182* + 
                 2.2 
                 1.9 
                 1.2 
                 1.1 
                 2.5 
                 1.9 
                 1.3 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + Q98S + G109A + A174S + G182* + 
                 1.9 
                 1.8 
                 1.2 
                 1.1 
                 2.1 
                 1.8 
                 1.1 
                 1.1 
               
               
                 D183* + N195F + V206L + R320A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.7 
                 1.1 
                 1.1 
                 2.1 
                 1.5 
                 1.2 
                 1.1 
               
               
                 N195F + V206L + H324L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + G149Q + A174S + G182* + 
                 1.7 
                 1.9 
                 1.2 
                 1.2 
                 1.6 
                 1.4 
                 1.0 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 2.0 
                 1.2 
                 1.2 
                 2.3 
                 1.7 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + G346T + K391A + G476K + G477A 
               
               
                 H1* + N54S + V56T + G109AG255A ++ A174S + G182* + 
                 2.1 
                 2.0 
                 1.2 
                 1.2 
                 2.3 
                 1.8 
                 1.2 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + G50A + N54S + V56T + G109A + F113L + R116L + 
                 2.3 
                 2.2 
                 1.2 
                 1.1 
                 2.6 
                 2.2 
                 1.3 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167F + A174S + G182* + 
                 2.0 
                 1.9 
                 1.1 
                 1.0 
                 2.3 
                 2.0 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + L173V + A174S + G182* + 
                 1.8 
                 1.9 
                 1.0 
                 1.0 
                 2.2 
                 1.8 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + W167F + A174S + 
                 2.1 
                 1.7 
                 1.1 
                 1.1 
                 2.9 
                 2.0 
                 1.4 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.4 
                 1.0 
                 1.1 
                 2.4 
                 1.9 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + K391A + G476K + G477Q 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.5 
                 1.0 
                 1.0 
                 2.2 
                 1.7 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + Q345D + K391A + G476K + G477Q 
               
               
                 H1* + G50A + N54S + V56T + G109A + Q172D + A174S + 
                 1.9 
                 1.9 
                 0.9 
                 1.1 
                 2.1 
                 1.9 
                 1.6 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.9 
                 0.7 
                 1.0 
                 2.2 
                 2.0 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + V264I + K391A + G423H + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 2.0 
                 0.8 
                 1.0 
                 2.1 
                 2.1 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + R320V + K391A + G476Y 
               
               
                 H1* + T5K + N54S + V56T + G109A + A174S + G182* + 
                 1.7 
                 1.8 
                 0.9 
                 1.0 
                 1.8 
                 1.7 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116M + A174S + G182* + 
                 2.9 
                 2.2 
                 1.2 
                 1.1 
                 2.8 
                 2.1 
                 1.2 
                 1.1 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172Y + A174S + G182* + 
                 2.1 
                 1.8 
                 1.1 
                 1.1 
                 1.9 
                 1.8 
                 1.4 
                 1.1 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.7 
                 1.1 
                 1.1 
                 1.9 
                 1.7 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + I214L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.9 
                 1.2 
                 1.2 
                 2.2 
                 2.2 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + L217V + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.7 
                 1.2 
                 1.2 
                 2.3 
                 2.1 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + L217H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 2.0 
                 1.1 
                 1.1 
                 2.3 
                 2.1 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + P211D + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.9 
                 1.1 
                 1.2 
                 2.3 
                 1.9 
                 1.3 
                 1.3 
               
               
                 N195F + V206L + K391A + K391Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.1 
                 1.1 
                 2.3 
                 1.9 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + K391A + T444H + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.7 
                 1.1 
                 1.0 
                 2.3 
                 1.8 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + K391A + T444V + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.6 
                 1.2 
                 1.1 
                 1.9 
                 1.8 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + T444R + G476K 
               
               
                 H1* + G50A + N54S + V56T + G109A + L173V + A174S + 
                 2.4 
                 2.1 
                 1.2 
                 1.2 
                 3.2 
                 2.2 
                 1.6 
                 1.4 
               
               
                 G182* + D183* + N195F + A204V + V206L + K391A + G476K 
               
               
                 H1* + G50A + N54S + V56T + G109A + F113L + W167F + 
                 1.9 
                 1.7 
                 1.0 
                 1.1 
                 2.1 
                 1.8 
                 1.2 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + G7K + N54S + V56T + G109A + A174S + G182* + 
                 2.0 
                 1.8 
                 1.1 
                 1.1 
                 2.7 
                 2.1 
                 1.6 
                 1.2 
               
               
                 D183* + N195F + V206L + R320A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118G + A174S + G182* + 
                 1.7 
                 1.8 
                 1.1 
                 1.1 
                 2.4 
                 1.6 
                 1.5 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.6 
                 1.0 
                 1.1 
                 1.6 
                 1.6 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + S280K + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.4 
                 1.6 
                 1.1 
                 1.1 
                 1.8 
                 1.6 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + N270G + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172D + A174S + G182* + 
                 2.1 
                 1.9 
                 1.2 
                 1.1 
                 3.1 
                 2.3 
                 1.6 
                 1.4 
               
               
                 D183* + N195F + V206L + G346T + K391A + G476K + G477A 
               
               
                 H1* + N54S + V56T + G109A + Q118S + A174S + G182* + 
                 2.1 
                 1.9 
                 1.1 
                 1.1 
                 1.8 
                 1.5 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.2 
                 1.2 
                 2.3 
                 1.7 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + R320A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + T134E + A174S + G182* + 
                 2.4 
                 2.1 
                 1.2 
                 1.1 
                 2.8 
                 2.4 
                 1.4 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.8 
                 1.0 
                 1.0 
                 1.8 
                 1.8 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + G337H + Q385L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.8 
                 1.1 
                 1.1 
                 2.2 
                 1.9 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + Y382M + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 1.9 
                 1.9 
                 1.1 
                 1.1 
                 2.7 
                 2.2 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + G255A + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + A174S + 
                 2.4 
                 2.0 
                 1.2 
                 1.1 
                 3.0 
                 2.3 
                 1.4 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + G255A + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + M105F + G109A + A174S + 
                 2.0 
                 1.7 
                 1.2 
                 1.1 
                 2.5 
                 2.0 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + G255A + K391A + G476K 
               
               
                 H1* + N54S + V56T + M105F + G109A + T134E + A174S + 
                 2.0 
                 1.9 
                 1.0 
                 1.0 
                 2.3 
                 2.1 
                 1.2 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + M105F + G109A + Q118F + T134E + 
                 1.9 
                 1.7 
                 1.1 
                 1.1 
                 1.7 
                 1.9 
                 1.3 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.1 
                 1.0 
                 2.3 
                 2.0 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + R320A + S323N + D377H + K391A + G476K 
               
               
                 H1* + T51E + N54S + V56T + G109A + A174H + G182* + 
                 2.3 
                 1.9 
                 1.2 
                 1.1 
                 2.6 
                 2.2 
                 1.6 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + L173A + A174S + G182* + 
                 2.4 
                 2.0 
                 1.3 
                 1.2 
                 2.4 
                 2.0 
                 1.6 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.5 
                 2.0 
                 1.3 
                 1.2 
                 2.4 
                 1.6 
                 1.6 
                 1.4 
               
               
                 N195F + V206L + Q299V + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + L173G + A174S + G182* + 
                 2.2 
                 2.0 
                 1.3 
                 1.2 
                 2.3 
                 1.9 
                 1.7 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 2.0 
                 1.3 
                 1.2 
                 2.4 
                 2.0 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + T355L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.3 
                 1.7 
                 1.0 
                 1.0 
                 1.9 
                 1.6 
                 1.2 
                 1.1 
               
               
                 N195F + V206L + T355F + K391A + G476K 
               
               
                 H1* + G50A + N54S + V56T + G109A + A174S + G182* + 
                 2.3 
                 2.0 
                 1.2 
                 1.1 
                 2.1 
                 1.8 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + A204T + V206L + K391A + G476K 
               
               
                 H1* + G50A + N54S + V56T + G109A + A174E + G182* + 
                 1.8 
                 1.6 
                 1.1 
                 1.1 
                 2.1 
                 1.8 
                 1.2 
                 1.2 
               
               
                 D183* + N195F + A204T + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 2.2 
                 1.8 
                 1.2 
                 1.1 
                 2.7 
                 2.1 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + V206L + R320A + S323N + K391A + G476K 
               
               
                 H1* + N54S + V56T + M105I + G109A + A174S + G182* + 
                 1.8 
                 1.8 
                 1.1 
                 1.1 
                 1.5 
                 1.5 
                 1.3 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.8 
                 1.1 
                 1.0 
                 1.8 
                 1.6 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + M246L + K391A + G476K 
               
               
                 H1* + T51G + N54S + V56T + G109A + A174N + G182* + 
                 1.6 
                 1.5 
                 1.0 
                 1.0 
                 2.1 
                 1.7 
                 1.3 
                 1.1 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51A + N54S + V56T + G109A + A174G + G182* + 
                 1.4 
                 1.5 
                 0.9 
                 0.9 
                 1.6 
                 1.5 
                 1.3 
                 1.0 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174D + G182* + D183* + 
                 1.9 
                 1.5 
                 1.0 
                 1.0 
                 1.5 
                 1.5 
                 1.1 
                 1.0 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + T51S + N54S + V56T + G109A + A174P + G182* + 
                 1.7 
                 1.7 
                 0.9 
                 1.0 
                 1.1 
                 1.2 
                 0.8 
                 0.9 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51G + N54S + V56T + G109A + A174M + G182* + 
                 1.3 
                 1.5 
                 1.0 
                 1.0 
                 1.6 
                 1.5 
                 0.9 
                 1.1 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + K72A + W167R + A174S + 
                 0.9 
                 1.2 
                 0.5 
                 0.7 
                 0.7 
                 0.8 
                 0.4 
                 0.7 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72A + G109A + W167S + A174S + 
                 1.6 
                 1.8 
                 0.4 
                 0.8 
                 0.7 
                 1.2 
                 0.6 
                 0.8 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + W167R + A174S + 
                 1.1 
                 1.1 
                 0.8 
                 0.8 
                 0.7 
                 0.8 
                 0.7 
                 0.7 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72Q + G109A + W167H + A174S + 
                 2.4 
                 2.2 
                 0.9 
                 1.0 
                 1.8 
                 1.7 
                 1.0 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118R + T134E + A174S + 
                 1.8 
                 1.7 
                 1.1 
                 1.0 
                 2.1 
                 1.7 
                 1.2 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + Q11H + N54S + V56T + G109A + Q118R + T134E + 
                 1.9 
                 1.6 
                 1.1 
                 1.0 
                 2.2 
                 1.6 
                 1.3 
                 1.3 
               
               
                 A174S + G184T + G182* + D183* + N195F + V206L + 
               
               
                 D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + A174S + 
                 2.3 
                 2.1 
                 1.2 
                 1.1 
                 3.2 
                 1.9 
                 1.3 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + A174S + 
                 2.2 
                 2.0 
                 1.1 
                 1.0 
                 3.8 
                 1.9 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + A174S + 
                 2.2 
                 2.0 
                 1.2 
                 1.1 
                 3.9 
                 2.4 
                 1.4 
                 1.3 
               
               
                 G182* + D183* + G184T + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118R + T134E + A174S + 
                 2.0 
                 1.9 
                 1.0 
                 1.2 
                 2.5 
                 2.0 
                 1.2 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + G255A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118R + A174S + G182* + 
                 2.2 
                 1.9 
                 1.1 
                 1.1 
                 2.8 
                 1.8 
                 1.2 
                 1.2 
               
               
                 D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + T134E + A174S + G182* + 
                 2.1 
                 2.1 
                 1.0 
                 1.1 
                 3.4 
                 2.3 
                 1.3 
                 1.3 
               
               
                 D183* + G184T + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172D + A174Q + G182* + 
                 2.0 
                 1.9 
                 1.2 
                 1.1 
                 3.0 
                 2.2 
                 1.1 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K + G477A 
               
               
                 H1* + N54S + V56T + K72A + G109A + W167F + A174S + 
                 1.1 
                 1.4 
                 0.6 
                 0.8 
                 1.5 
                 1.3 
                 1.0 
                 0.8 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167S + G182* + D183* + 
                 1.4 
                 1.3 
                 0.8 
                 1.0 
                 1.5 
                 1.6 
                 1.3 
                 1.1 
               
               
                 N195F + A174S + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72H + G109A + W167S + A174S + 
                 1.4 
                 1.4 
                 0.5 
                 1.0 
                 1.0 
                 1.4 
                 0.8 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + W167T + A174S + 
                 1.3 
                 1.2 
                 0.8 
                 0.8 
                 1.2 
                 1.0 
                 1.1 
                 1.0 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72E + G109A + A174S + G182* + 
                 1.3 
                 1.4 
                 0.8 
                 0.9 
                 1.4 
                 1.3 
                 0.9 
                 0.9 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + W167L + A174S + 
                 1.6 
                 1.6 
                 1.1 
                 1.1 
                 2.0 
                 1.5 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + W167M + A174S + 
                 1.5 
                 1.6 
                 0.8 
                 1.0 
                 1.5 
                 1.3 
                 1.2 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + W167H + A174S + 
                 1.3 
                 1.4 
                 0.6 
                 1.0 
                 1.6 
                 1.3 
                 0.9 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 2.0 
                 1.9 
                 1.2 
                 1.1 
                 2.5 
                 2.0 
                 1.4 
                 1.3 
               
               
                 D183* + G184T + N195F + V206L + G255A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.9 
                 1.1 
                 1.1 
                 2.3 
                 1.9 
                 1.5 
                 1.4 
               
               
                 G184T + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 2.0 
                 1.2 
                 1.0 
                 2.1 
                 1.7 
                 1.3 
                 1.3 
               
               
                 N195F + V206L + G255A + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118R + A174S + G182* + 
                 1.2 
                 1.5 
                 1.0 
                 1.0 
                 1.8 
                 1.2 
                 1.0 
                 1.0 
               
               
                 D183* + N195F + V206L + G255A + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.2 
                 1.3 
                 0.9 
                 1.0 
                 1.3 
                 1.3 
                 1.0 
                 1.0 
               
               
                 G184A + T193K + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + M105F + G109A + A174S + G182* + 
                 1.9 
                 2.0 
                 1.1 
                 1.2 
                 2.1 
                 1.9 
                 1.4 
                 1.3 
               
               
                 D183* + G184T + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + T134E + A174S + G182* + 
                 1.7 
                 2.1 
                 1.1 
                 1.1 
                 2.1 
                 2.0 
                 1.3 
                 1.3 
               
               
                 D183* + N195F + V206L + R320A + S323N + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.8 
                 1.2 
                 1.1 
                 2.1 
                 1.9 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + V291G + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.9 
                 1.1 
                 1.1 
                 2.1 
                 1.9 
                 1.3 
                 1.3 
               
               
                 N195F + V206L + V291A + F328L + D377H + K391A + G476K 
               
               
                 H1* + V56T + G109A + A174S + G182* + D183* + N195F + 
                 1.3 
                 1.8 
                 1.1 
                 1.1 
                 1.5 
                 1.5 
                 1.3 
                 1.2 
               
               
                 V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + T134E + A174S + G182* + 
                 2.4 
                 2.2 
                 1.1 
                 1.1 
                 3.0 
                 2.4 
                 1.5 
                 1.3 
               
               
                 D183* + G184T + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.8 
                 1.2 
                 1.1 
                 2.1 
                 1.9 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + V238A + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.8 
                 1.1 
                 1.1 
                 2.5 
                 1.9 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + V238T + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.6 
                 1.1 
                 1.1 
                 2.3 
                 1.8 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + V238G + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.8 
                 1.0 
                 1.0 
                 2.1 
                 1.8 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + F328M + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.7 
                 0.9 
                 1.0 
                 1.7 
                 1.5 
                 1.0 
                 1.1 
               
               
                 N195F + V206L + F328L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.6 
                 0.8 
                 1.0 
                 1.8 
                 1.7 
                 1.0 
                 1.1 
               
               
                 N195F + V206L + F328V + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.6 
                 0.9 
                 1.1 
                 2.0 
                 1.6 
                 1.1 
                 1.0 
               
               
                 N195F + V206L + F328I + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.8 
                 1.2 
                 1.1 
                 2.4 
                 1.9 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + V291T + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.4 
                 1.9 
                 1.2 
                 1.2 
                 2.9 
                 2.1 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + V291A + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + A174S + 
                 2.1 
                 1.9 
                 1.0 
                 1.1 
                 2.1 
                 2.0 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + G184T + N195F + V206L + G255A + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + L173H + A174S + G182* + 
                 2.0 
                 1.8 
                 1.2 
                 1.1 
                 2.6 
                 2.0 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q125T + A174S + G182* + 
                 1.4 
                 1.7 
                 0.9 
                 1.0 
                 2.0 
                 1.8 
                 1.2 
                 1.0 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N16S + N54S + V56T + G109A + A174S + G182* + 
                 1.9 
                 1.6 
                 1.0 
                 1.0 
                 2.1 
                 1.9 
                 1.3 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72N + G109A + A174S + G182* + 
                 2.1 
                 1.6 
                 1.0 
                 1.0 
                 2.1 
                 2.2 
                 1.3 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72N + G109A + W167L + A174S + 
                 2.2 
                 1.9 
                 0.9 
                 1.2 
                 1.9 
                 2.1 
                 1.2 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72A + G109A + W167Y + A174S + 
                 1.7 
                 1.8 
                 0.8 
                 1.1 
                 1.6 
                 2.0 
                 1.1 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118R + T134E + A174S + 
                 2.0 
                 1.9 
                 1.0 
                 1.1 
                 2.0 
                 2.0 
                 1.2 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + G255A + D377H + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 2.2 
                 2.1 
                 1.1 
                 1.1 
                 3.3 
                 2.5 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + G184T + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q118R + A174S + 
                 0.9 
                 1.3 
                 0.8 
                 0.9 
                 1.3 
                 1.5 
                 1.0 
                 1.1 
               
               
                 G182* + D183* + G184T + N195F + V206L + D377H + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q118R + A174S + 
                 2.4 
                 2.1 
                 1.2 
                 1.2 
                 2.8 
                 2.2 
                 1.5 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + G255A + D377H + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.8 
                 1.2 
                 1.1 
                 2.5 
                 2.0 
                 1.5 
                 1.4 
               
               
                 G184T + N195F + V206L + R320A + S323N + K391A + G476K 
               
               
                 H1* + N54S + G109A + A174S + G182* + D183* + N195F + 
                 1.5 
                 1.7 
                 1.1 
                 1.1 
                 2.3 
                 1.8 
                 1.4 
                 1.3 
               
               
                 V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + A174S + G182* + D183* + N195F + 
                 1.8 
                 1.7 
                 1.1 
                 0.9 
                 2.1 
                 1.7 
                 1.4 
                 1.2 
               
               
                 V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + G182* + D183* + N195F + 
                 2.0 
                 1.8 
                 1.2 
                 1.2 
                 2.6 
                 1.7 
                 1.3 
                 1.2 
               
               
                 V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.6 
                 1.3 
                 1.1 
                 2.8 
                 2.1 
                 1.5 
                 1.2 
               
               
                 N195F + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.6 
                 1.1 
                 1.1 
                 2.6 
                 2.0 
                 1.3 
                 1.1 
               
               
                 N195F + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 2.0 
                 1.2 
                 1.1 
                 2.6 
                 1.9 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + D377H + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.2 
                 1.1 
                 2.5 
                 1.8 
                 1.6 
                 1.4 
               
               
                 N195F + V206L + D377H + K391A 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.4 
                 1.6 
                 1.1 
                 1.0 
                 1.5 
                 1.7 
                 1.3 
                 1.0 
               
               
                 N195F + V206L + D377R + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.5 
                 1.7 
                 1.1 
                 1.0 
                 2.3 
                 1.2 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + L217T + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.8 
                 1.3 
                 1.2 
                 2.4 
                 1.8 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + D377S + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116E + A174S + G182* + 
                 2.0 
                 1.9 
                 1.2 
                 1.1 
                 2.3 
                 2.0 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.5 
                 1.7 
                 1.0 
                 1.1 
                 1.7 
                 1.4 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + L217Q + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.3 
                 1.5 
                 1.0 
                 1.0 
                 1.7 
                 1.6 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + G346D + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.7 
                 1.1 
                 1.2 
                 2.5 
                 1.7 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + D377A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + T134E + A174S + G182* + 
                 2.2 
                 2.1 
                 1.2 
                 1.1 
                 2.3 
                 2.1 
                 1.1 
                 1.2 
               
               
                 D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118R + T134E + A174S + 
                 1.9 
                 1.7 
                 1.3 
                 1.2 
                 2.0 
                 1.7 
                 1.2 
                 1.2 
               
               
                 G182* + D183* + G184T + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q118R + T134E + 
                 1.9 
                 1.7 
                 1.1 
                 1.1 
                 2.1 
                 1.9 
                 1.0 
                 1.1 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.6 
                 1.2 
                 1.1 
                 2.3 
                 1.9 
                 1.2 
                 1.1 
               
               
                 N195F + V206L + G346P + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116S + A174S + G182* + 
                 2.0 
                 1.8 
                 1.2 
                 1.1 
                 2.1 
                 1.9 
                 1.1 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.8 
                 1.2 
                 1.1 
                 2.0 
                 2.0 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + D377Q + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116I + A174S + G182* + 
                 2.2 
                 1.8 
                 1.2 
                 1.1 
                 2.1 
                 2.0 
                 1.3 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174F + G182* + D183* + 
                 1.7 
                 1.5 
                 1.1 
                 1.0 
                 1.5 
                 1.6 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116G + A174S + G182* + 
                 2.0 
                 1.9 
                 1.2 
                 1.2 
                 2.1 
                 2.0 
                 1.0 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174T + G182* + D183* + 
                 1.9 
                 1.8 
                 1.2 
                 1.2 
                 1.9 
                 2.0 
                 1.1 
                 1.2 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118R + T134E + A174S + 
                 1.5 
                 1.8 
                 1.1 
                 1.0 
                 1.9 
                 1.9 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + G184T + N195F + V206L + G255A + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q118R + T134E + 
                 1.7 
                 1.8 
                 1.1 
                 1.0 
                 2.2 
                 1.8 
                 1.5 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + G255A + 
               
               
                 K391A + G476K 
               
               
                 H1* + T51G + N54S + V56T + G109A + Q172R + A174S + 
                 1.2 
                 1.6 
                 1.0 
                 1.0 
                 1.6 
                 1.4 
                 1.3 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51A + N54S + V56T + G109A + Q172D + A174S + 
                 1.5 
                 1.7 
                 1.0 
                 1.0 
                 2.3 
                 1.9 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51S + N54S + V56T + G109A + Q172M + A174S + 
                 1.7 
                 1.6 
                 1.0 
                 1.0 
                 2.0 
                 1.8 
                 1.2 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51A + N54S + V56T + G109A + Q172S + A174S + 
                 2.4 
                 1.8 
                 1.3 
                 1.1 
                 2.8 
                 2.1 
                 1.7 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51E + N54S + V56T + G109A + Q172T + A174S + 
                 2.0 
                 1.8 
                 0.9 
                 1.0 
                 2.6 
                 1.9 
                 1.6 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51A + N54S + V56T + G109A + Q172A + A174S + 
                 1.9 
                 1.8 
                 1.0 
                 1.1 
                 2.3 
                 1.9 
                 1.6 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51S + N54S + V56T + G109A + Q172N + A174S + 
                 2.2 
                 1.9 
                 1.1 
                 1.1 
                 2.4 
                 1.9 
                 1.5 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51G + N54S + V56T + G109A + Q172K + A174S + 
                 1.4 
                 1.3 
                 1.0 
                 0.9 
                 1.7 
                 1.5 
                 1.2 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51A + N54S + V56T + G109A + Q172G + A174S + 
                 1.7 
                 1.7 
                 1.2 
                 1.0 
                 2.2 
                 1.9 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51E + N54S + V56T + G109A + Q172L + A174S + 
                 2.0 
                 1.9 
                 1.0 
                 1.1 
                 2.2 
                 1.8 
                 1.2 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51E + N54S + V56T + G109A + Q172R + A174S + 
                 2.0 
                 1.9 
                 1.1 
                 1.1 
                 1.8 
                 1.7 
                 1.4 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172K + A174S + G182* + 
                 2.1 
                 1.8 
                 1.0 
                 1.1 
                 2.0 
                 1.6 
                 1.3 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51D + N54S + V56T + G109A + Q172N + A174S + 
                 1.8 
                 1.7 
                 1.0 
                 1.0 
                 1.9 
                 1.6 
                 1.3 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51A + N54S + V56T + G109A + Q172K + A174S + 
                 1.7 
                 1.7 
                 1.2 
                 1.1 
                 1.8 
                 1.4 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q172S + A174S + 
                 2.0 
                 1.8 
                 1.3 
                 1.1 
                 2.6 
                 1.8 
                 1.4 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72H + G109A + Q172T + A174S + 
                 2.0 
                 1.8 
                 1.2 
                 1.1 
                 2.0 
                 1.9 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q172R + A174S + 
                 1.8 
                 1.6 
                 1.1 
                 1.1 
                 1.4 
                 1.5 
                 1.1 
                 1.1 
               
               
                 K179E + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q172M + A174S + 
                 1.9 
                 1.8 
                 1.2 
                 1.1 
                 2.1 
                 1.7 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + Y295N + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q172R + A174S + 
                 1.4 
                 1.4 
                 1.0 
                 1.0 
                 1.5 
                 1.4 
                 1.2 
                 0.9 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72Q + G109A + Q172R + A174S + 
                 2.0 
                 2.0 
                 1.0 
                 1.0 
                 2.6 
                 2.1 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q172T + A174S + 
                 2.1 
                 2.1 
                 1.3 
                 1.1 
                 2.3 
                 2.0 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72M + G109A + Q172S + A174S + 
                 2.3 
                 1.9 
                 1.2 
                 1.1 
                 2.4 
                 2.0 
                 1.2 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72Q + G109A + Q172M + A174S + 
                 2.1 
                 2.1 
                 1.3 
                 1.1 
                 2.8 
                 2.2 
                 1.5 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172M + A174S + G182* + 
                 1.9 
                 2.0 
                 1.2 
                 1.1 
                 2.4 
                 1.7 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72S + G109A + A174H + G182* + 
                 2.3 
                 2.0 
                 1.2 
                 1.1 
                 2.2 
                 2.1 
                 1.4 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72Q + G109A + A174D + G182* + 
                 2.3 
                 2.1 
                 1.1 
                 1.1 
                 2.2 
                 1.9 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174D + G182* + 
                 2.5 
                 2.0 
                 1.1 
                 1.1 
                 2.3 
                 2.1 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72Q + G109A + A174K + G182* + 
                 2.2 
                 1.9 
                 1.2 
                 1.1 
                 2.6 
                 2.0 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174G + G182* + D183* + 
                 2.3 
                 1.9 
                 1.2 
                 1.1 
                 2.6 
                 2.2 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174Q + G182* + 
                 2.5 
                 2.1 
                 1.2 
                 1.2 
                 2.3 
                 2.1 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72Q + G109A + A174G + G182* + 
                 2.3 
                 2.1 
                 1.1 
                 1.1 
                 2.6 
                 2.2 
                 1.4 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72S + G109A + A174N + G182* + 
                 2.6 
                 2.1 
                 1.3 
                 1.1 
                 2.6 
                 2.3 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174H + G182* + 
                 2.1 
                 1.9 
                 1.3 
                 1.2 
                 2.5 
                 2.2 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.7 
                 1.1 
                 1.1 
                 1.9 
                 1.6 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + K391A + K391S + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.6 
                 1.2 
                 1.1 
                 2.2 
                 1.6 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + K391A + T444A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113L + W167F + A174S + 
                 1.7 
                 1.6 
                 1.0 
                 1.0 
                 2.1 
                 1.7 
                 1.2 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + G50A + N54S + V56T + G109A + A174E + G182* + 
                 2.0 
                 1.8 
                 1.0 
                 1.1 
                 1.5 
                 1.7 
                 1.3 
                 1.2 
               
               
                 D183* + N195F + A204T + V206L + S323N + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q118R + A174S + 
                 1.6 
                 1.6 
                 1.1 
                 1.0 
                 1.2 
                 1.3 
                 1.2 
                 1.2 
               
               
                 G182* + D183* + G184T + N195F + V206L + G255A + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + M105F + G109A + A174S + G182* + 
                 1.8 
                 1.9 
                 1.2 
                 1.1 
                 2.6 
                 1.9 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q118R + A174S + 
                 1.5 
                 1.6 
                 1.1 
                 1.1 
                 1.4 
                 1.3 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.7 
                 2.1 
                 1.2 
                 1.2 
                 2.9 
                 2.6 
                 1.6 
                 1.4 
               
               
                 N195F + A204G + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.8 
                 1.0 
                 0.9 
                 1.8 
                 1.7 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + A225F + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.6 
                 0.9 
                 0.9 
                 1.7 
                 1.8 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + M246A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.7 
                 0.9 
                 0.9 
                 1.8 
                 1.5 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + Y382I + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.7 
                 1.1 
                 0.9 
                 2.1 
                 2.0 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + S376H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.5 
                 1.1 
                 0.9 
                 2.1 
                 2.1 
                 1.2 
                 1.3 
               
               
                 N195F + V206L + I214E + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.9 
                 1.1 
                 1.1 
                 2.4 
                 2.0 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + I214W + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + Q172K + A174S + 
                 1.7 
                 1.4 
                 0.9 
                 1.0 
                 2.1 
                 1.7 
                 1.2 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.9 
                 1.1 
                 1.1 
                 2.7 
                 2.1 
                 1.5 
                 1.3 
               
               
                 N195F + A204T + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.6 
                 1.2 
                 1.1 
                 2.2 
                 1.8 
                 1.3 
                 1.3 
               
               
                 N195F + A204S + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + T165S + A174S + G182* + 
                 1.9 
                 1.8 
                 1.1 
                 1.1 
                 2.3 
                 2.0 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.6 
                 1.0 
                 1.0 
                 2.3 
                 1.7 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + T334S + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.6 
                 1.0 
                 1.0 
                 2.1 
                 1.6 
                 1.4 
                 1.1 
               
               
                 N195F + V206L + A263G + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.6 
                 1.1 
                 1.0 
                 2.3 
                 1.7 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + M286F + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.6 
                 1.0 
                 1.0 
                 1.8 
                 1.7 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + Y267I + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.8 
                 1.1 
                 1.0 
                 2.5 
                 2.1 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + L235V + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.6 
                 0.8 
                 0.9 
                 2.3 
                 1.9 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + L235A + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + M105L + G109A + A174S + G182* + 
                 2.1 
                 1.9 
                 1.1 
                 1.1 
                 2.9 
                 2.1 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + M105I + G109A + A174S + G182* + 
                 2.2 
                 2.0 
                 1.1 
                 1.1 
                 2.3 
                 2.0 
                 1.6 
                 1.4 
               
               
                 D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + M105V + G109A + A174S + G182* + 
                 2.3 
                 2.1 
                 1.2 
                 1.1 
                 2.6 
                 2.1 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.9 
                 1.3 
                 1.1 
                 2.6 
                 2.0 
                 1.7 
                 1.3 
               
               
                 N195F + V206L + M246I + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 2.0 
                 1.3 
                 1.1 
                 2.3 
                 1.9 
                 1.6 
                 1.3 
               
               
                 N195F + V206L + L250I + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.9 
                 1.3 
                 1.2 
                 2.5 
                 2.0 
                 1.6 
                 1.3 
               
               
                 N195F + V206L + L250V + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.9 
                 1.2 
                 1.1 
                 2.1 
                 1.9 
                 1.6 
                 1.4 
               
               
                 N195F + V206L + K391Y + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.9 
                 1.0 
                 1.0 
                 2.1 
                 1.8 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + K391V + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.8 
                 1.3 
                 1.1 
                 2.6 
                 2.1 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + K391M + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.6 
                 1.2 
                 1.0 
                 2.1 
                 1.9 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + K391E + G476K 
               
               
                 H1* + N54S + V56T + G109A + T165G + A174S + G182* + 
                 2.3 
                 2.0 
                 1.3 
                 1.1 
                 2.9 
                 2.0 
                 1.7 
                 1.3 
               
               
                 D183* + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54R + V56T + G109A + A174S + G182* + D183* + 
                 1.3 
                 1.4 
                 1.1 
                 1.1 
                 1.1 
                 1.1 
                 1.1 
                 0.8 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54L + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.4 
                 1.1 
                 1.0 
                 1.3 
                 1.6 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109S + A174S + G182* + D183* + 
                 2.4 
                 2.4 
                 1.4 
                 1.2 
                 2.3 
                 2.0 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174V + G182* + D183* + 
                 2.2 
                 2.2 
                 1.3 
                 1.3 
                 2.3 
                 2.0 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.6 
                 2.3 
                 1.3 
                 1.1 
                 2.5 
                 2.0 
                 1.6 
                 1.2 
               
               
                 N195F + V206L + M246F + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.8 
                 1.3 
                 1.2 
                 1.9 
                 1.8 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + M246V + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 1.9 
                 1.2 
                 1.2 
                 1.8 
                 1.6 
                 1.4 
                 1.1 
               
               
                 N195F + V206L + M246S + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174R + G182* + D183* + 
                 1.7 
                 1.6 
                 1.2 
                 1.1 
                 1.7 
                 1.6 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174L + G182* + D183* + 
                 1.9 
                 1.7 
                 1.2 
                 1.2 
                 2.2 
                 1.7 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.6 
                 1.0 
                 1.1 
                 2.0 
                 1.7 
                 1.3 
                 1.3 
               
               
                 N195F + V206S + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 2.0 
                 1.3 
                 1.2 
                 2.2 
                 2.0 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + K391D + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 1.8 
                 1.3 
                 1.2 
                 2.5 
                 1.9 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + K391R + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.9 
                 1.2 
                 1.2 
                 2.3 
                 2.0 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + K391H + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.6 
                 1.3 
                 1.2 
                 2.5 
                 2.0 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + K391W + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.8 
                 1.2 
                 1.2 
                 2.0 
                 1.8 
                 1.3 
                 1.3 
               
               
                 N195F + V206L + K391I + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.6 
                 1.1 
                 1.1 
                 2.6 
                 2.0 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + L250T + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.8 
                 1.0 
                 1.0 
                 2.6 
                 2.0 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + L250A + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.7 
                 1.1 
                 1.1 
                 2.4 
                 2.0 
                 1.6 
                 1.2 
               
               
                 N195F + V206L + L250F + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.7 
                 1.1 
                 1.1 
                 2.0 
                 1.9 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + L250M + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.4 
                 1.1 
                 1.0 
                 1.5 
                 1.7 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + K391Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172H + L173A + A174P + 
                 2.5 
                 1.8 
                 1.3 
                 1.2 
                 2.3 
                 2.1 
                 1.6 
                 1.4 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172E + L173A + A174R + 
                 1.1 
                 1.4 
                 0.7 
                 1.0 
                 1.8 
                 1.7 
                 1.2 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72Q + G109A + W167R + A174S + 
                 1.9 
                 1.7 
                 1.2 
                 1.1 
                 1.6 
                 1.6 
                 1.0 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.7 
                 1.1 
                 1.0 
                 2.0 
                 1.7 
                 1.3 
                 1.2 
               
               
                 G184T + N195F + V206L + G255A + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 1.7 
                 1.6 
                 1.1 
                 1.1 
                 1.9 
                 1.6 
                 1.1 
                 1.0 
               
               
                 D183* + G184T + N195F + V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.4 
                 2.1 
                 1.3 
                 1.2 
                 2.3 
                 2.0 
                 1.4 
                 1.2 
               
               
                 G184T + N195F + V206L + G255A + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118R + A174S + G182* + 
                 1.6 
                 1.5 
                 1.1 
                 1.2 
                 0.7 
                 1.1 
                 0.8 
                 1.0 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 2.0 
                 1.3 
                 1.2 
                 2.3 
                 1.8 
                 1.3 
                 1.3 
               
               
                 N195F + V206L + S376T + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.8 
                 1.2 
                 1.2 
                 2.1 
                 1.8 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + S376V + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172E + L173I + A174N + 
                 2.3 
                 1.9 
                 1.1 
                 1.1 
                 2.1 
                 1.9 
                 1.1 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172D + L173A + A174T + 
                 2.3 
                 2.0 
                 1.1 
                 1.1 
                 2.2 
                 2.0 
                 1.1 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 2.0 
                 1.2 
                 1.2 
                 2.6 
                 2.0 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + Y267M + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.6 
                 1.1 
                 1.0 
                 2.5 
                 1.9 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + Y267H + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.5 
                 1.1 
                 1.0 
                 2.3 
                 1.7 
                 1.2 
                 1.1 
               
               
                 N195F + V206L + V238A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.5 
                 1.3 
                 1.1 
                 0.9 
                 1.7 
                 1.5 
                 1.2 
                 1.1 
               
               
                 N195F + V206L + V238T + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 1.7 
                 1.2 
                 1.1 
                 3.0 
                 2.1 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + V238G + K391A + G476K 
               
               
                 H1* + N54S + V56T + M105V + G109A + A174S + G182* + 
                 2.0 
                 1.7 
                 1.2 
                 1.1 
                 2.3 
                 2.1 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 2.1 
                 1.2 
                 1.0 
                 2.9 
                 1.9 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + V264T + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.4 
                 1.8 
                 1.3 
                 1.1 
                 2.9 
                 2.3 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + V264I + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.7 
                 1.1 
                 1.0 
                 2.4 
                 1.9 
                 1.3 
                 1.3 
               
               
                 N195F + V206L + L235V + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167H + Q172N + L173A + 
                 2.0 
                 1.7 
                 1.2 
                 1.1 
                 2.9 
                 2.1 
                 1.4 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167F + Q172E + L173P + 
                 1.7 
                 1.9 
                 1.2 
                 1.1 
                 3.3 
                 2.2 
                 1.5 
                 1.3 
               
               
                 A174K + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172N + L173T + A174S + 
                 2.0 
                 1.7 
                 1.2 
                 1.1 
                 2.8 
                 2.0 
                 1.4 
                 1.4 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172K + L173V + A174T + 
                 1.6 
                 1.4 
                 1.0 
                 1.1 
                 1.6 
                 1.6 
                 1.0 
                 1.0 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172E + L173F + A174R + 
                 1.9 
                 1.6 
                 1.1 
                 1.0 
                 2.0 
                 1.9 
                 1.3 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172E + L173T + A174R + 
                 1.9 
                 1.8 
                 1.2 
                 1.2 
                 2.5 
                 2.1 
                 0.9 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172N + L173V + A174R + 
                 1.9 
                 1.7 
                 1.3 
                 1.2 
                 2.2 
                 1.8 
                 0.9 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + L173V + A174R + G182* + 
                 1.7 
                 1.8 
                 1.3 
                 1.2 
                 1.7 
                 1.6 
                 0.8 
                 1.1 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172E + L173M + A174H + 
                 2.1 
                 2.1 
                 1.3 
                 1.2 
                 2.8 
                 2.1 
                 1.3 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172E + L173F + A174P + 
                 1.9 
                 1.9 
                 1.2 
                 1.2 
                 2.7 
                 2.3 
                 0.8 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167Y + Q172E + L173V + 
                 2.0 
                 1.8 
                 1.1 
                 1.0 
                 2.8 
                 2.0 
                 1.4 
                 1.3 
               
               
                 A174H + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + L173A + A174T + G182* + 
                 2.0 
                 1.8 
                 1.0 
                 1.0 
                 2.4 
                 1.8 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.6 
                 1.1 
                 1.0 
                 2.9 
                 1.7 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + V291A + F328L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.7 
                 1.0 
                 1.0 
                 2.8 
                 1.7 
                 1.2 
                 1.2 
               
               
                 N195F + V206L + M246L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.8 
                 1.2 
                 1.1 
                 2.9 
                 2.0 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + M246S + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.3 
                 1.2 
                 2.8 
                 2.0 
                 1.6 
                 1.4 
               
               
                 N195F + V206L + L250V + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.8 
                 1.1 
                 1.0 
                 2.9 
                 2.1 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + L250F + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 1.7 
                 1.2 
                 1.1 
                 3.0 
                 2.1 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + L250M + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.8 
                 1.3 
                 1.0 
                 2.8 
                 2.0 
                 1.3 
                 1.3 
               
               
                 N195F + V206L + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.6 
                 1.0 
                 1.0 
                 1.7 
                 1.8 
                 1.4 
                 1.3 
               
               
                 N195F + A204S + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + T165G + A174S + G182* + 
                 2.3 
                 1.9 
                 1.2 
                 1.1 
                 2.9 
                 2.2 
                 1.3 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.7 
                 1.0 
                 1.0 
                 2.6 
                 2.0 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + T334S + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.1 
                 1.3 
                 1.0 
                 0.9 
                 1.4 
                 1.3 
                 1.1 
                 1.1 
               
               
                 N195F + V206L + A263G + M286F + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.5 
                 1.1 
                 1.0 
                 2.2 
                 1.9 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + A263G + M286L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.9 
                 1.2 
                 1.2 
                 2.7 
                 2.2 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + Y267H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.8 
                 1.0 
                 1.1 
                 2.4 
                 2.2 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + Y267L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.2 
                 1.5 
                 0.7 
                 0.9 
                 1.7 
                 1.9 
                 1.3 
                 1.3 
               
               
                 N195F + V206L + Y267I + K391A + G476K 
               
               
                 H1* + T51A + N54S + V56T + G109A + Q172R + A174S + 
                 2.1 
                 1.5 
                 1.1 
                 1.0 
                 1.5 
                 1.6 
                 1.4 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.4 
                 1.9 
                 1.1 
                 1.1 
                 2.5 
                 1.9 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + K391L + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + Q172G + 
                 2.6 
                 2.1 
                 1.1 
                 1.0 
                 3.1 
                 2.1 
                 1.4 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + Q172G + A174S + 
                 1.9 
                 1.8 
                 0.9 
                 0.9 
                 2.7 
                 1.9 
                 1.2 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109S + F113Q + R116Q + A174S + 
                 2.9 
                 2.2 
                 1.2 
                 1.1 
                 3.3 
                 2.6 
                 1.5 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172D + A174S + G182* + 
                 2.7 
                 2.2 
                 1.2 
                 1.1 
                 3.1 
                 2.1 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + A204G + V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + A174S + 
                 2.8 
                 2.3 
                 1.2 
                 1.1 
                 3.4 
                 2.4 
                 1.7 
                 1.3 
               
               
                 G182* + D183* + N195F + A204G + V206L + G255A + 
               
               
                 K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + W167F + A174S + 
                 2.7 
                 2.2 
                 1.2 
                 1.1 
                 3.1 
                 2.2 
                 1.6 
                 1.3 
               
               
                 G182* + D183* + N195F + A204G + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 2.3 
                 2.1 
                 1.2 
                 1.1 
                 2.7 
                 2.0 
                 1.4 
                 1.3 
               
               
                 D183* + G184T + N195F + A204G + V206L + K391A + 
               
               
                 P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167F + Q172N + A174S + 
                 2.5 
                 1.8 
                 1.3 
                 1.2 
                 2.6 
                 1.8 
                 1.6 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167F + Q172G + A174S + 
                 2.4 
                 2.0 
                 1.3 
                 1.2 
                 2.8 
                 2.0 
                 1.6 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + P473G + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167F + Q172G + A174S + 
                 2.1 
                 1.8 
                 1.3 
                 1.1 
                 2.3 
                 1.8 
                 1.5 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + W167F + Q172R + 
                 2.2 
                 1.9 
                 1.1 
                 1.1 
                 2.0 
                 1.8 
                 1.2 
                 1.1 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + R116H + W167F + 
                 2.4 
                 1.8 
                 1.2 
                 1.1 
                 2.4 
                 1.9 
                 1.5 
                 1.2 
               
               
                 Q172R + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + M105F + G109A + A174S + G182* + 
                 2.6 
                 2.2 
                 1.3 
                 1.3 
                 3.4 
                 2.4 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + R320A + S323N + K391A + 
               
               
                 C474V + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 2.4 
                 2.1 
                 1.4 
                 1.3 
                 3.2 
                 2.2 
                 1.7 
                 1.3 
               
               
                 D183* + N195F + V206L + G255A + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + T134E + A174S + G182* + 
                 2.8 
                 2.3 
                 1.3 
                 1.2 
                 3.7 
                 2.3 
                 1.6 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.6 
                 2.0 
                 1.3 
                 1.2 
                 3.0 
                 2.1 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + D377H + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 2.8 
                 2.1 
                 1.3 
                 1.2 
                 3.3 
                 2.4 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.9 
                 1.0 
                 1.1 
                 2.6 
                 2.0 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + A265G + K391A + T444Q + G476K + G477A 
               
               
                 H1* + A37V + N54S + V56T + G109A + W167F + Q172G + 
                 2.3 
                 2.0 
                 1.2 
                 1.2 
                 2.8 
                 2.2 
                 1.5 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 G346T + G477A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116Q + Q172D + A174S + 
                 2.3 
                 2.1 
                 0.9 
                 1.1 
                 3.1 
                 2.4 
                 1.4 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + G346T + K391A + 
               
               
                 T444Q + G477A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116Q + W167F + Q172N + 
                 2.2 
                 2.2 
                 1.1 
                 1.2 
                 2.7 
                 2.4 
                 1.3 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + G346T + 
               
               
                 K391A + T444Q + G477A + G476K 
               
               
                 H1* + N54S + V56T + G109S + W167F + Q172R + A174S + 
                 1.9 
                 1.4 
                 1.2 
                 1.1 
                 2.1 
                 1.6 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109S + R116Q + W167F + Q172N + 
                 2.8 
                 2.2 
                 1.4 
                 1.2 
                 3.2 
                 3.0 
                 1.5 
                 1.5 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109S + W167F + Q172G + A174S + 
                 2.6 
                 1.9 
                 1.4 
                 1.1 
                 3.2 
                 2.2 
                 1.6 
                 1.5 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + Q172G + A174S + 
                 2.5 
                 2.0 
                 1.5 
                 1.2 
                 3.2 
                 2.2 
                 1.5 
                 1.4 
               
               
                 G182* + D183* + N195F + V206L + K391A + T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + Q172G + 
                 2.6 
                 2.2 
                 1.3 
                 1.1 
                 3.5 
                 2.6 
                 1.7 
                 1.4 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + A174S + G182* + 
                 2.5 
                 2.0 
                 1.3 
                 1.1 
                 3.1 
                 2.6 
                 1.6 
                 1.5 
               
               
                 D183* + N195F + V206L + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172N + A174S + G182* + 
                 2.4 
                 1.7 
                 1.4 
                 1.1 
                 3.2 
                 2.4 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + V264I + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172N + A174S + G182* + 
                 2.5 
                 1.7 
                 1.3 
                 1.1 
                 2.0 
                 2.1 
                 1.3 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.8 
                 1.2 
                 1.1 
                 2.9 
                 2.1 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + S280Q + H321Y + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + A174S + 
                 2.1 
                 1.8 
                 1.2 
                 1.0 
                 3.3 
                 2.3 
                 1.6 
                 1.4 
               
               
                 G182* + D183* + N195F + V206L + G255A + K391A + 
               
               
                 C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + Q172G + 
                 2.4 
                 2.0 
                 1.2 
                 1.1 
                 3.3 
                 2.3 
                 1.7 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 T444Q + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116Q + Q172G + 
                 2.8 
                 2.1 
                 1.3 
                 1.1 
                 3.1 
                 2.3 
                 1.5 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116H + W167F + Q172N + 
                 2.4 
                 2.0 
                 1.2 
                 1.1 
                 2.8 
                 2.2 
                 1.5 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + T165G + 
                 2.4 
                 2.0 
                 1.2 
                 1.1 
                 3.1 
                 2.2 
                 1.6 
                 1.2 
               
               
                 Q172G + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116Q + T165G + 
                 2.5 
                 2.1 
                 1.3 
                 1.2 
                 3.4 
                 2.3 
                 1.7 
                 1.4 
               
               
                 Q172G + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + W167F + 
                 2.3 
                 1.8 
                 1.2 
                 1.1 
                 2.6 
                 2.0 
                 1.4 
                 1.3 
               
               
                 Q172R + A174S + G182* + D183* + N195F + V206L + 
               
               
                 F289I + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + Q172N + A174S + 
                 2.6 
                 2.2 
                 1.3 
                 1.2 
                 3.3 
                 2.3 
                 1.6 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + Q172N + 
                 2.6 
                 2.0 
                 1.3 
                 1.2 
                 3.2 
                 2.2 
                 1.7 
                 1.4 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116Q + Q172M + 
                 2.2 
                 2.0 
                 1.2 
                 1.1 
                 2.8 
                 2.2 
                 1.3 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + Q172M + 
                 2.2 
                 1.9 
                 1.2 
                 1.0 
                 3.0 
                 2.4 
                 1.5 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116H + Q172M + A174S + 
                 2.3 
                 2.1 
                 1.1 
                 1.0 
                 2.7 
                 2.1 
                 1.5 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + T165G + 
                 1.9 
                 1.7 
                 1.2 
                 1.1 
                 2.6 
                 1.8 
                 1.5 
                 1.3 
               
               
                 Q172M + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + Q395P + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + A174S + G182* + 
                 1.7 
                 1.3 
                 0.9 
                 1.0 
                 1.9 
                 1.6 
                 0.9 
                 1.0 
               
               
                 D183* + N195F + V206L + K391A + T444Q + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116Q + A174S + G182* + 
                 2.4 
                 2.2 
                 1.3 
                 1.2 
                 2.7 
                 2.4 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + A265G + K391A + T444Q + 
               
               
                 P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116Q + A174S + G182* + 
                 2.4 
                 2.1 
                 1.3 
                 1.1 
                 2.9 
                 2.3 
                 1.3 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + T444Q + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + T165G + 
                 2.5 
                 2.1 
                 1.2 
                 1.1 
                 3.0 
                 2.3 
                 1.4 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + G255A + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + W167F + 
                 2.1 
                 2.0 
                 1.2 
                 1.2 
                 2.9 
                 2.3 
                 1.5 
                 1.3 
               
               
                 Q172R + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116Q + W167F + Q172R + 
                 2.0 
                 1.8 
                 1.2 
                 1.1 
                 2.2 
                 2.0 
                 1.3 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + A265G + 
               
               
                 K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + A174S + G182* + 
                 2.1 
                 1.9 
                 1.0 
                 1.0 
                 2.6 
                 2.0 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172N + A174S + G182* + 
                 2.0 
                 1.7 
                 1.2 
                 1.1 
                 2.5 
                 1.9 
                 1.5 
                 1.1 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + T51V + N54S + V56T + G109A + Q172D + A174S + 
                 2.4 
                 1.9 
                 1.2 
                 1.1 
                 2.6 
                 1.9 
                 1.6 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.3 
                 1.9 
                 1.2 
                 1.1 
                 2.5 
                 1.8 
                 1.5 
                 1.2 
               
               
                 N195F + V206L + S280Q + H321Y + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + Q172N + 
                 2.6 
                 2.3 
                 1.3 
                 1.2 
                 3.1 
                 2.4 
                 1.7 
                 1.4 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + Q172G + 
                 2.3 
                 2.0 
                 1.3 
                 1.2 
                 2.9 
                 2.3 
                 1.6 
                 1.3 
               
               
                 L173V + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + T444Q + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116Q + W167F + Q172N + 
                 2.6 
                 2.2 
                 1.3 
                 1.1 
                 3.3 
                 2.5 
                 1.8 
                 1.3 
               
               
                 L173V + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + G133Q + W167F + Q172N + 
                 2.5 
                 2.3 
                 1.3 
                 1.2 
                 3.3 
                 2.4 
                 1.8 
                 1.4 
               
               
                 A174S + G182* + D183* + N195F + V206L + Q395P + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + W167F + 
                 2.3 
                 2.0 
                 1.2 
                 1.2 
                 2.8 
                 2.3 
                 1.6 
                 1.3 
               
               
                 Q172N + L173V + A174S + G182* + D183* + N195F + 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + A37V + N54S + V56T + K72R + G109A + R116H + 
                 2.5 
                 2.2 
                 1.3 
                 1.1 
                 3.3 
                 2.5 
                 1.7 
                 1.2 
               
               
                 W167F + Q172R + A174S + G182* + D183* + G184T + 
               
               
                 N195F + V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116H + 
                 2.2 
                 2.2 
                 1.2 
                 1.1 
                 3.1 
                 2.4 
                 1.7 
                 1.3 
               
               
                 W167F + Q172G + L173V + A174S + G182* + D183* + 
               
               
                 G184T + N195F + V206L + K391A + T444Q + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109S + R116Q + W167F + Q172R + 
                 2.5 
                 2.2 
                 1.2 
                 1.0 
                 3.1 
                 2.5 
                 1.6 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109S + R116Q + W167F + Q172G + 
                 2.5 
                 2.1 
                 1.2 
                 1.1 
                 3.0 
                 2.3 
                 1.6 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 P473R + G476K 
               
               
                 H1* + N54S + V56T + G109S + F113Q + W167F + Q172N + 
                 2.7 
                 2.4 
                 1.4 
                 1.3 
                 3.1 
                 2.5 
                 1.7 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + A37V + N54S + V56T + G109S + F113Q + W167F + 
                 2.8 
                 2.4 
                 1.3 
                 1.2 
                 3.5 
                 2.5 
                 1.7 
                 1.4 
               
               
                 Q172R + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + R116H + T134E + 
                 2.2 
                 1.9 
                 1.2 
                 1.1 
                 2.7 
                 2.4 
                 1.6 
                 1.3 
               
               
                 W167F + Q172G + L173V + A174S + G182* + D183* + 
               
               
                 N195F + V206L + G255A + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + R116H + T134E + 
                 2.5 
                 2.0 
                 1.2 
                 1.1 
                 3.5 
                 2.5 
                 1.6 
                 1.3 
               
               
                 W167F + Q172G + L173V + A174S + G182* + D183* + 
               
               
                 N195F + V206L + G255A + K391A + Q395P + T444Q + 
               
               
                 P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116Q + 
                 2.5 
                 2.1 
                 1.3 
                 1.1 
                 3.4 
                 2.5 
                 1.6 
                 1.3 
               
               
                 W167F + Q172G + A174S + G182* + D183* + G184T + 
               
               
                 N195F + V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116Q + 
                 2.3 
                 2.0 
                 1.1 
                 1.1 
                 3.1 
                 2.6 
                 1.5 
                 1.3 
               
               
                 T134E + W167F + Q172R + A174S + G182* + D183* + 
               
               
                 N195F + V206L + G255A + K391A + T444Q + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + T134E + 
                 1.9 
                 1.9 
                 1.1 
                 1.1 
                 2.7 
                 2.1 
                 1.6 
                 1.3 
               
               
                 W167F + Q172R + A174S + G182* + D183* + N195F + 
               
               
                 V206L + G255A + K391A + Q395P + A445Q + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116H + 
                 2.1 
                 2.2 
                 1.3 
                 1.2 
                 2.9 
                 2.4 
                 1.6 
                 1.4 
               
               
                 T134E + W167F + Q172R + A174S + G182* + D183* + 
               
               
                 N195F + V206L + G255A + K391A + Q395P + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + W167F + 
                 2.6 
                 2.4 
                 1.3 
                 1.2 
                 3.2 
                 2.5 
                 1.7 
                 1.4 
               
               
                 Q172N + A174S + G182* + D183* + N195F + V206L + 
               
               
                 G255A + A265G + K391A + T444Q + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + G133Q + W167F + 
                 2.4 
                 2.2 
                 1.3 
                 1.2 
                 3.2 
                 2.4 
                 1.6 
                 1.4 
               
               
                 Q172R + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116Q + 
                 2.9 
                 2.3 
                 1.3 
                 1.2 
                 3.7 
                 2.8 
                 1.8 
                 1.4 
               
               
                 W167F + Q172G + A174S + G182* + D183* + N195F + 
               
               
                 A204G + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116Q + 
                 2.0 
                 2.0 
                 1.3 
                 1.2 
                 2.7 
                 2.1 
                 1.5 
                 1.3 
               
               
                 W167F + Q172N + L173V + A174S + G182* + D183* + 
               
               
                 G184T + N195F + V206L + K391A + T444Q + P473R + G476K 
               
               
                 H1* + A37H + N54S + V56T + G109A + F113Q + W167F + 
                 2.5 
                 1.9 
                 1.2 
                 1.1 
                 2.6 
                 1.8 
                 1.6 
                 1.3 
               
               
                 Q172N + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + G476K 
               
               
                 H1* + A37H + N54S + V56T + G109A + F113Q + A174S + 
                 1.9 
                 1.7 
                 1.3 
                 1.2 
                 2.8 
                 2.0 
                 1.3 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + W167F + Q172N + 
                 2.3 
                 1.6 
                 1.3 
                 1.2 
                 2.8 
                 1.8 
                 1.5 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 T459N + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + Q172R + L173V + 
                 1.9 
                 1.5 
                 1.1 
                 1.0 
                 2.6 
                 1.5 
                 1.3 
                 1.1 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116Q + A174S + 
                 2.3 
                 2.2 
                 1.1 
                 1.1 
                 3.2 
                 2.3 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + A60V + G109A + R116Q + W167F + 
                 2.4 
                 2.2 
                 1.2 
                 1.2 
                 3.3 
                 2.4 
                 1.3 
                 1.4 
               
               
                 Q172N + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + A60V + G109A + R116H + Q172R + 
                 2.7 
                 2.0 
                 1.4 
                 1.2 
                 3.2 
                 2.5 
                 1.3 
                 1.4 
               
               
                 L173V + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + G476K 
               
               
                 H1* + A37V + N54S + V56T + A60V + G109A + R116Q + 
                 2.5 
                 1.9 
                 1.1 
                 1.2 
                 2.9 
                 2.1 
                 1.1 
                 1.3 
               
               
                 T165G + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + G476K 
               
               
                 H1* + A37H + N54S + V56T + A60V + G109A + R116Q + 
                 2.7 
                 2.2 
                 1.2 
                 1.2 
                 3.6 
                 2.5 
                 1.7 
                 1.4 
               
               
                 T165G + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + A60V + G109A + R116Q + W167F + 
                 2.3 
                 2.3 
                 1.0 
                 1.2 
                 2.5 
                 2.3 
                 1.2 
                 1.3 
               
               
                 Q172G + L173V + A174S + G182* + D183* + N195F + 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + A60V + G109A + R116Q + A174S + 
                 2.3 
                 2.1 
                 0.9 
                 1.1 
                 3.1 
                 2.4 
                 1.4 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + A37H + N54S + V56T + A60V + G109A + R116Q + 
                 3.0 
                 2.4 
                 1.2 
                 1.1 
                 3.6 
                 2.4 
                 1.7 
                 1.3 
               
               
                 W167F + Q172R + A174S + G182* + D183* + N195F + 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + A37H + N54S + V56T + A60V + G109A + R116Q + 
                 2.7 
                 2.7 
                 1.1 
                 1.1 
                 3.0 
                 2.4 
                 1.4 
                 1.3 
               
               
                 W167F + Q172N + A174S + G182* + D183* + N195F + 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + A60V + G109A + R116Q + T165G + 
                 2.4 
                 2.2 
                 1.2 
                 1.2 
                 3.0 
                 2.2 
                 1.3 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 T444Q + P473R + G476K 
               
               
                 H1* + N54S + V56T + A60V + G109A + R116Q + A174S + 
                 2.5 
                 1.8 
                 0.9 
                 1.0 
                 2.2 
                 2.0 
                 1.1 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + T444Q + 
               
               
                 P473R + G476K 
               
               
                 H1* + N54S + V56T + A60V + G109A + R116Q + W167F + 
                 2.7 
                 2.8 
                 1.0 
                 1.1 
                 3.3 
                 2.7 
                 1.6 
                 1.4 
               
               
                 Q172N + L173V + A174S + G182* + D183* + N195F + 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + A60V + G109A + R116Q + W167F + 
                 2.8 
                 2.5 
                 1.3 
                 1.2 
                 3.6 
                 2.7 
                 1.7 
                 1.4 
               
               
                 Q172R + A174S + G182* + D183* + N195F + V206L + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116A + W167F + Q172R + 
                 2.3 
                 2.0 
                 1.1 
                 1.1 
                 2.5 
                 2.0 
                 1.6 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + A204G + V206L + 
               
               
                 K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116A + A174S + G182* + 
                 2.8 
                 2.5 
                 1.3 
                 1.2 
                 3.4 
                 2.3 
                 1.6 
                 1.5 
               
               
                 D183* + N195F + A204G + V206L + K391A + P473R + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + R116A + T165G + A174S + 
                 2.5 
                 2.1 
                 1.2 
                 1.1 
                 2.8 
                 2.0 
                 1.6 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + T444Q + 
               
               
                 P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + G182* + D183* + 
                 2.2 
                 2.2 
                 1.2 
                 1.1 
                 2.3 
                 2.1 
                 1.5 
                 1.5 
               
               
                 N195F + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + G182* + D183* + 
                 2.2 
                 2.0 
                 1.3 
                 1.2 
                 2.4 
                 1.9 
                 1.5 
                 1.4 
               
               
                 N195F + V206L 
               
               
                 N54S + V56T + G109A + G182* + D183* + N195F 
                 2.2 
                 2.0 
                 1.3 
                 1.2 
                 2.3 
                 2.0 
                 1.5 
                 1.4 
               
               
                 N54S + V56T + G109A + A174S + G182* + D183* + N195F + 
                 2.4 
                 2.0 
                 1.3 
                 1.2 
                 2.5 
                 2.0 
                 1.5 
                 1.4 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + G109A + A174S + G182* + D183* + N195F + V206L + 
                 1.9 
                 1.9 
                 1.3 
                 1.2 
                 2.4 
                 2.0 
                 1.5 
                 1.3 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + G182* + D183* + N195F + 
                 2.2 
                 1.9 
                 1.2 
                 1.1 
                 2.5 
                 1.7 
                 1.3 
                 1.3 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.2 
                 1.1 
                 2.2 
                 1.8 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + K391A 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.2 
                 1.1 
                 2.5 
                 1.9 
                 1.4 
                 1.3 
               
               
                 N195F + G476K 
               
               
                 H1* + N54S + V56T + A174S + G182* + D183* + N195F + 
                 2.3 
                 2.0 
                 1.2 
                 1.1 
                 2.6 
                 2.1 
                 1.4 
                 1.4 
               
               
                 V206L + G273R + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.4 
                 2.1 
                 1.3 
                 1.1 
                 2.7 
                 2.1 
                 1.6 
                 1.4 
               
               
                 N195F + V206L 
               
               
                 H1* + W48G + G109A + G182* + D183* + N195F + V206L + 
                 1.7 
                 1.6 
                 1.2 
                 1.1 
                 2.2 
                 1.7 
                 1.3 
                 1.2 
               
               
                 K391A + G476K 
               
               
                 N54S + V56T + G109A + A174S + G182* + D183* + N195F + 
                 2.0 
                 1.7 
                 1.2 
                 1.2 
                 2.9 
                 2.2 
                 1.5 
                 1.3 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.4 
                 1.5 
                 1.1 
                 1.1 
                 2.3 
                 1.8 
                 1.3 
                 1.3 
               
               
                 N195F + K391A 
               
               
                 H1* + N54S + V56T + G109A + F113Q + Q172M + A174S + 
                 2.0 
                 1.9 
                 1.2 
                 1.1 
                 2.9 
                 2.2 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116Q + A174S + G182* + 
                 1.8 
                 1.6 
                 0.9 
                 1.0 
                 1.7 
                 1.8 
                 1.0 
                 1.1 
               
               
                 D183* + N195F + A204G + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + T134E + 
                 2.5 
                 2.2 
                 1.2 
                 1.2 
                 2.9 
                 2.5 
                 1.4 
                 1.4 
               
               
                 A174S + G182* + D183* + N195F + V206L + G255A + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + T134E + 
                 2.7 
                 2.3 
                 1.3 
                 1.2 
                 3.3 
                 2.5 
                 1.7 
                 1.4 
               
               
                 A174S + G182* + D183* + N195F + V206L + G255A + 
               
               
                 K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + R116Q + A174S + 
                 2.7 
                 2.2 
                 1.2 
                 1.2 
                 3.4 
                 2.4 
                 1.6 
                 1.3 
               
               
                 G182* + D183* + G184T + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + A37V + N54S + V56T + G109A + R116A + T165G + 
                 2.4 
                 2.2 
                 1.2 
                 1.2 
                 3.1 
                 2.5 
                 1.5 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + A37V + N54S + V56T + G109A + R116Q + T165G + 
                 2.5 
                 2.2 
                 1.2 
                 1.2 
                 3.0 
                 2.3 
                 1.5 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + M246F + 
               
               
                 K391A + G476K 
               
               
                 H1* + V56A + G109A + A174S + G182* + D183* + 
                 2.0 
                 2.0 
                 1.1 
                 1.1 
                 2.5 
                 2.1 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + G476K 
               
               
                 H1* + N54S + V56T + G109A + G182* + D183* + 
                 1.8 
                 2.0 
                 1.2 
                 1.0 
                 2.2 
                 1.9 
                 1.6 
                 1.4 
               
               
                 N195F + V206L + K391A 
               
               
                 H1* + N54S + V56T + G109A + G182* + D183* + 
                 1.8 
                 1.7 
                 1.0 
                 1.1 
                 1.9 
                 1.9 
                 1.4 
                 1.3 
               
               
                 N195F + V206L + G476K 
               
               
                 G109A + A174S + G182* + D183* + N195F + V206L + 
                 1.5 
                 1.6 
                 1.0 
                 1.0 
                 1.7 
                 1.7 
                 1.2 
                 1.2 
               
               
                 K391A + G476K 
               
               
                 N54S + V56T + G109A + G182* + D183* + N195F + 
                 1.7 
                 1.7 
                 1.2 
                 1.0 
                 1.6 
                 1.7 
                 1.4 
                 1.3 
               
               
                 V206L + K391T + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116H + 
                 2.4 
                 2.2 
                 1.2 
                 1.2 
                 3.4 
                 2.3 
                 1.4 
                 1.4 
               
               
                 A174S + G182* + D183* + G184T + N195F + V206L + 
               
               
                 K391A + T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109S + F113Q + A174S + G182* + 
                 2.4 
                 2.0 
                 1.3 
                 1.2 
                 3.1 
                 2.2 
                 1.6 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + T444Q + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + W167F + 
                 2.9 
                 2.3 
                 1.3 
                 1.2 
                 3.8 
                 2.6 
                 1.8 
                 1.4 
               
               
                 Q172G + L173V + A174S + G182* + D183* + N195F + 
               
               
                 V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116Q + Q172N + 
                 2.8 
                 2.3 
                 1.2 
                 1.2 
                 3.5 
                 2.6 
                 1.7 
                 1.5 
               
               
                 A174S + G182* + D183* + N195F + V206L + A265G + 
               
               
                 K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + W167F + Q172N + 
                 2.5 
                 2.0 
                 1.2 
                 1.2 
                 2.9 
                 2.2 
                 1.7 
                 1.4 
               
               
                 A174S + G182* + D183* + N195F + A204G + V206L + 
               
               
                 K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + W167F + 
                 2.1 
                 1.9 
                 1.2 
                 1.1 
                 2.3 
                 1.8 
                 1.3 
                 1.3 
               
               
                 Q172R + A174S + G182* + D183* + N195F + A204G + 
               
               
                 V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116A + A174S + G182* + 
                 2.1 
                 1.9 
                 1.1 
                 1.1 
                 2.7 
                 2.2 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + T444Q + P473R + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + R116H + Q172R + A174S + 
                 2.2 
                 1.6 
                 1.2 
                 1.1 
                 2.5 
                 1.8 
                 1.5 
                 1.2 
               
               
                 G182* + D183* + N195F + A204G + V206L + K391A + 
               
               
                 P473R + G476K 
               
               
                 H1* + A37V + N54S + V56T + G109A + R116Q + T165G + 
                 2.3 
                 2.0 
                 1.2 
                 1.1 
                 3.0 
                 2.1 
                 1.5 
                 1.4 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + A37H + N54S + V56T + G109A + R116H + T165G + 
                 2.3 
                 2.0 
                 1.3 
                 1.1 
                 3.2 
                 2.2 
                 1.5 
                 1.4 
               
               
                 A174S + G182* + D183* + N195F + V206L + M246F + 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.9 
                 1.1 
                 1.1 
                 2.1 
                 1.9 
                 1.2 
                 1.2 
               
               
                 N195F + K391A + G476K 
               
               
                 H1* + G109A + G182* + D183* + N195F + V206L + 
                 1.7 
                 1.6 
                 1.1 
                 1.1 
                 1.8 
                 1.5 
                 1.3 
                 1.2 
               
               
                 K391A + G476K 
               
               
                 G109A + G182* + D183* + N195F + V206L + G476K 
                 1.9 
                 1.7 
                 1.2 
                 1.1 
                 2.1 
                 1.5 
                 1.2 
                 1.1 
               
               
                 H1* + G109A + G182* + D183* + N195F + K391A 
                 1.9 
                 1.9 
                 1.2 
                 1.2 
                 1.6 
                 1.7 
                 1.3 
                 1.3 
               
               
                 H1* + N54S + V56T + G109A + G133S + A174S + G182* + 
                 2.2 
                 1.9 
                 1.2 
                 1.1 
                 2.4 
                 2.0 
                 1.3 
                 1.3 
               
               
                 D183* + N195F + K391A + G476H 
               
               
                 H1* + N54S + V56T + G109A + R116A + Q172N + L173V + 
                 2.5 
                 2.3 
                 1.2 
                 1.2 
                 3.2 
                 2.5 
                 1.6 
                 1.4 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + A37V + N54S + V56T + G109A + R116H + T165G + 
                 2.5 
                 2.1 
                 1.1 
                 1.2 
                 2.6 
                 2.3 
                 1.4 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + P45A + N54S + V56T + G109A + A174S + G182* + 
                 2.3 
                 2.1 
                 1.3 
                 1.2 
                 3.1 
                 2.1 
                 1.5 
                 1.4 
               
               
                 D183* + N195F + V206L + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + P124A + A174S + G182* + 
                 1.9 
                 2.2 
                 1.3 
                 1.2 
                 2.8 
                 2.2 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 2.0 
                 1.3 
                 1.2 
                 2.4 
                 2.1 
                 1.5 
                 1.4 
               
               
                 N195F + V206L + P364A + K391A + C474V + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q118R + T134E + A174S + 
                 3.7 
                 2.5 
                 1.6 
                 1.2 
                 3.2 
                 2.6 
                 1.5 
                 1.6 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 3.4 
                 2.4 
                 1.6 
                 1.3 
                 3.9 
                 2.7 
                 1.6 
                 1.7 
               
               
                 G184T + N195F + V206L + K391A + G476K 
               
               
                 H1* + G50A + T51A + N54S + V56T + G109A + A174S + 
                 3.2 
                 2.2 
                 1.5 
                 1.2 
                 3.1 
                 2.4 
                 1.8 
                 1.5 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 3.2 
                 2.3 
                 1.6 
                 1.3 
                 3.4 
                 2.3 
                 1.7 
                 1.5 
               
               
                 N195F + V206L + V264F + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 3.0 
                 2.2 
                 1.6 
                 1.2 
                 2.9 
                 2.4 
                 1.4 
                 1.4 
               
               
                 N195F + V206L + Y382L + K391A + G476K 
               
               
                 H1* + G7K + N54S + V56T + G109A + A174S + G182* + 
                 3.7 
                 2.2 
                 1.5 
                 1.1 
                 3.7 
                 2.6 
                 1.7 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 3.0 
                 2.2 
                 1.5 
                 1.2 
                 3.2 
                 2.6 
                 1.7 
                 1.5 
               
               
                 N195F + V206L + G255A + Q256A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167Y + A174S + G182* + 
                 3.4 
                 2.3 
                 1.4 
                 1.3 
                 3.0 
                 2.4 
                 1.8 
                 1.5 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W167H + A174S + G182* + 
                 2.9 
                 2.0 
                 1.4 
                 1.1 
                 2.5 
                 2.1 
                 1.8 
                 1.5 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + T165V + A174S + G182* + 
                 2.6 
                 2.0 
                 1.4 
                 1.2 
                 2.5 
                 2.2 
                 1.6 
                 1.5 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 3.2 
                 2.4 
                 1.3 
                 1.2 
                 3.0 
                 2.1 
                 1.9 
                 1.5 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174N + G182* + D183* + 
                 2.8 
                 2.2 
                 1.4 
                 1.2 
                 2.7 
                 1.8 
                 1.4 
                 1.4 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174Q + G182* + D183* + 
                 3.5 
                 2.0 
                 1.6 
                 1.2 
                 2.4 
                 2.1 
                 1.8 
                 1.5 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N16H + V17L + N54S + V56T + G109A + A174S + 
                 2.7 
                 1.9 
                 1.3 
                 1.2 
                 4.6 
                 2.3 
                 1.6 
                 1.4 
               
               
                 G182* + D183* + N195F + V206L + Y382F + K391A + 
               
               
                 G476K 
               
               
                 H1* + Q32S + N54S + V56T + G109A + A174S + G182* + 
                 2.7 
                 1.8 
                 1.3 
                 1.1 
                 3.5 
                 2.2 
                 1.4 
                 1.3 
               
               
                 D183* + N195F + V206L + Q385L + K391A + G476K 
               
               
                 H1* + N54S + V56T + R87S + G109A + R171H + A174S + 
                 2.5 
                 1.9 
                 1.1 
                 1.1 
                 3.4 
                 2.1 
                 1.4 
                 1.4 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 N54S + G182* + D183* + N195F 
                 1.1 
                 1.1 
                 1.1 
                 1.0 
                 1.6 
                 1.5 
                 1.0 
                 1.0 
               
               
                 G109A + G182* + D183* + N195F 
                 1.6 
                 1.4 
                 1.3 
                 1.0 
                 1.8 
                 1.4 
                 1.0 
                 1.1 
               
               
                 A174S + G182* + D183* + N195F 
                 0.8 
                 1.1 
                 1.3 
                 0.9 
                 1.2 
                 1.2 
                 0.9 
                 1.0 
               
               
                 G182* + D183* + N195F + V206L 
                 0.7 
                 0.7 
                 1.0 
                 1.0 
                 0.9 
                 1.3 
                 0.8 
                 1.0 
               
               
                 G182* + D183* + N195F + K391A 
                 1.6 
                 1.4 
                 1.3 
                 1.0 
                 1.4 
                 1.5 
                 1.0 
                 1.1 
               
               
                 G182* + D183* + N195F + G476K 
                 1.3 
                 1.4 
                 1.3 
                 1.0 
                 1.8 
                 1.5 
                 1.1 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + R116H + Q169E + Q172K + 
                 3.5 
                 1.9 
                 1.2 
                 1.1 
                 3.4 
                 2.2 
                 1.6 
                 1.2 
               
               
                 A174* + G182* + D183* + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + A174* + G182* + D183* + 
                 3.4 
                 2.1 
                 1.3 
                 1.1 
                 3.2 
                 2.1 
                 1.5 
                 1.1 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + K72R + G109A + F113Q + R116Q + W167F + Q172G + 
                 3.1 
                 2.0 
                 1.1 
                 1.0 
                 3.0 
                 2.1 
                 1.7 
                 1.4 
               
               
                 A174S + G182* + D183* + G184T + N195F + 
               
               
                 V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116Q + 
                 3.1 
                 1.9 
                 1.2 
                 1.0 
                 3.0 
                 1.9 
                 1.6 
                 1.3 
               
               
                 W167F + Q172G + A174S + G182* + D183* + G184T + 
               
               
                 N195F + V206L + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172G + A174* + 
                 3.0 
                 1.9 
                 1.1 
                 1.0 
                 2.7 
                 2.0 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116Q + 
                 2.9 
                 1.8 
                 1.1 
                 1.0 
                 2.9 
                 1.9 
                 1.4 
                 1.1 
               
               
                 W167F + Q172G + A174S + G182* + D183* + G184T + 
               
               
                 N195F + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116W + Q169E + Q172K + 
                 2.8 
                 1.9 
                 1.1 
                 1.0 
                 3.2 
                 2.1 
                 1.5 
                 1.1 
               
               
                 A174* + G182* + D183* + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.7 
                 1.8 
                 1.3 
                 1.0 
                 3.3 
                 2.0 
                 1.7 
                 1.2 
               
               
                 G182* + D183* + N195F + V206F + K391A + G476K 
               
               
                 H1* + V56T + G109A + A174S + G182* + D183* + N195F + 
                 2.6 
                 1.6 
                 1.2 
                 1.1 
                 2.4 
                 1.6 
                 1.4 
                 1.1 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.6 
                 1.7 
                 1.2 
                 1.0 
                 3.4 
                 2.2 
                 1.4 
                 1.1 
               
               
                 N195F + V206L + N260G + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 2.6 
                 1.5 
                 1.2 
                 1.0 
                 3.1 
                 1.8 
                 1.6 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.5 
                 1.8 
                 1.2 
                 1.0 
                 2.2 
                 1.7 
                 1.6 
                 1.2 
               
               
                 G182* + D183* + G184T + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.4 
                 1.6 
                 1.1 
                 1.0 
                 2.6 
                 1.9 
                 1.5 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + Y295N + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + W167F + A174S + G182* + 
                 2.4 
                 1.7 
                 1.2 
                 1.0 
                 2.5 
                 1.9 
                 1.7 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.4 
                 1.6 
                 1.4 
                 1.0 
                 4.9 
                 1.8 
                 1.8 
                 1.2 
               
               
                 G182* + D183* + A186N + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.4 
                 1.6 
                 1.1 
                 1.0 
                 2.4 
                 1.6 
                 1.3 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + A288V + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.4 
                 1.9 
                 1.2 
                 1.0 
                 3.2 
                 2.2 
                 2.0 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + G255A + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + G109A + Q169E + Q172K + A174* + G182* + 
                 2.3 
                 1.7 
                 1.1 
                 1.1 
                 3.2 
                 2.2 
                 1.5 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + W48F + N54S + V56T + G109A + Q169E + Q172K + 
                 2.3 
                 1.6 
                 1.1 
                 1.0 
                 2.3 
                 1.6 
                 1.4 
                 1.2 
               
               
                 A174* + G182* + D183* + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.3 
                 1.8 
                 1.2 
                 1.1 
                 1.8 
                 1.8 
                 1.6 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + V291A + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + A174S + G182* + 
                 2.3 
                 1.7 
                 1.3 
                 1.0 
                 2.3 
                 2.0 
                 1.7 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.3 
                 1.6 
                 1.2 
                 1.0 
                 3.3 
                 2.0 
                 1.6 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + R320A + S323N + 
               
               
                 K391A + G476K 
               
               
                 H1* + V56T + G109A + Q169E + Q172K + A174* + G182* + 
                 2.3 
                 1.6 
                 1.2 
                 0.9 
                 2.0 
                 1.7 
                 1.6 
                 1.1 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116H + 
                 2.3 
                 1.7 
                 1.1 
                 1.0 
                 2.0 
                 1.7 
                 1.7 
                 1.2 
               
               
                 W167F + Q172G + A174S + G182* + D183* + G184T + 
               
               
                 N195F + V206L + K391A + P473R + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.2 
                 1.7 
                 1.1 
                 1.0 
                 2.6 
                 2.1 
                 1.8 
                 1.4 
               
               
                 N195F + V206L + N270T + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.2 
                 1.6 
                 1.2 
                 1.1 
                 2.4 
                 1.6 
                 1.3 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.2 
                 1.6 
                 1.1 
                 1.0 
                 3.2 
                 2.0 
                 1.7 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + N260G + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + A174S + 
                 2.2 
                 1.7 
                 1.1 
                 1.1 
                 3.4 
                 2.1 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172G + A174S + G182* + 
                 2.2 
                 1.8 
                 1.2 
                 1.1 
                 2.3 
                 1.9 
                 1.6 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + A186D + G182* + 
                 2.1 
                 1.7 
                 1.2 
                 1.0 
                 2.2 
                 1.7 
                 1.5 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.1 
                 1.8 
                 1.0 
                 0.9 
                 2.0 
                 2.0 
                 1.9 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + A225V + K391A + 
               
               
                 G476K 
               
               
                 H1* + G109A + Q169E + Q172K + A174* + G182* + 
                 2.1 
                 1.5 
                 1.1 
                 0.9 
                 1.8 
                 1.4 
                 1.3 
                 1.1 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.1 
                 1.6 
                 1.2 
                 1.0 
                 3.3 
                 2.1 
                 1.6 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + G255S + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.1 
                 1.7 
                 1.0 
                 1.1 
                 2.0 
                 1.8 
                 1.4 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K269Q + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.1 
                 1.5 
                 1.1 
                 1.0 
                 2.0 
                 1.5 
                 1.2 
                 1.0 
               
               
                 N195F + V206L + G255S + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174S + 
                 2.1 
                 1.6 
                 1.1 
                 1.1 
                 2.9 
                 2.1 
                 1.5 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 G182* + D183* + N195F 
                 2.0 
                 1.7 
                 1.2 
                 0.9 
                 2.9 
                 1.8 
                 1.8 
                 1.2 
               
               
                 H1* + N54S + V56T + G109A + Q169E + A174S + G182* + 
                 2.0 
                 1.6 
                 1.1 
                 1.1 
                 2.0 
                 1.8 
                 1.6 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.0 
                 1.5 
                 1.1 
                 1.0 
                 2.8 
                 1.9 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + W284H + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.5 
                 1.1 
                 1.0 
                 2.3 
                 1.8 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 2.0 
                 1.5 
                 1.4 
                 1.0 
                 5.8 
                 1.7 
                 1.9 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + S304R + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116Q + 
                 2.0 
                 1.6 
                 1.2 
                 1.1 
                 2.0 
                 1.6 
                 1.3 
                 1.1 
               
               
                 W167F + Q172G + A174S + G182* + D183* + G184T + 
               
               
                 N195F + V206L + K391A + P473R 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 2.0 
                 1.7 
                 1.2 
                 1.1 
                 1.9 
                 1.9 
                 1.5 
                 1.3 
               
               
                 N195F + V206L + M246V + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 1.9 
                 1.4 
                 1.2 
                 1.1 
                 2.7 
                 2.0 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + K391A 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 1.9 
                 1.5 
                 1.1 
                 1.0 
                 2.2 
                 1.7 
                 1.4 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + N270G + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 1.9 
                 1.6 
                 1.2 
                 1.0 
                 2.7 
                 1.8 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + Y295F + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.5 
                 1.2 
                 1.1 
                 2.9 
                 1.8 
                 1.3 
                 1.3 
               
               
                 N195F + V206L + R320A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174T + 
                 1.9 
                 1.9 
                 1.2 
                 1.0 
                 2.4 
                 2.1 
                 1.6 
                 1.4 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + G182* + 
                 1.9 
                 1.7 
                 1.1 
                 1.0 
                 2.6 
                 1.7 
                 1.8 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + W140Y + Q169E + Q172K + 
                 1.9 
                 1.4 
                 1.1 
                 1.0 
                 2.1 
                 1.8 
                 1.5 
                 1.4 
               
               
                 A174* + G182* + D183* + N195F + V206L + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.9 
                 1.6 
                 1.1 
                 1.1 
                 2.2 
                 1.9 
                 1.7 
                 1.4 
               
               
                 N195F + V206L + K391A 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 1.8 
                 1.6 
                 1.1 
                 1.0 
                 1.7 
                 1.4 
                 1.5 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + F328L + K391A + 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 2.0 
                 1.5 
                 1.1 
                 5.2 
                 2.0 
                 1.8 
                 1.5 
               
               
                 N195F + V206L + G476K 
               
               
                 F113Q + R116H + D183* + G182* + N195F 
                 1.8 
                 1.7 
                 1.1 
                 1.0 
                 1.9 
                 1.9 
                 1.7 
                 1.3 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.3 
                 1.1 
                 1.0 
                 2.6 
                 1.8 
                 1.5 
                 1.2 
               
               
                 N195F + V206L + A288L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.6 
                 1.2 
                 1.1 
                 1.9 
                 1.8 
                 1.6 
                 1.2 
               
               
                 N195F + V206L + N270G + K391A + G476K 
               
               
                 Q169E + Q172K + A174* + D183* + G182* + N195F 
                 1.8 
                 1.2 
                 1.2 
                 1.0 
                 3.3 
                 1.4 
                 1.5 
                 1.3 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.8 
                 1.0 
                 1.1 
                 0.9 
                 1.8 
                 1.4 
                 1.5 
                 1.1 
               
               
                 N195F + V206L + K269Q + K391A + G476K 
               
               
                 H1* + W48F + N54S + V56T + G109A + A174S + G182* + 
                 1.8 
                 1.4 
                 1.1 
                 1.0 
                 2.2 
                 1.7 
                 1.5 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 F113Q + R116Q + D183* + G182* + N195F 
                 1.8 
                 1.7 
                 1.1 
                 1.0 
                 2.1 
                 1.7 
                 1.7 
                 1.4 
               
               
                 N54S + V56T + D183* + G182* + N195F 
                 1.8 
                 1.2 
                 0.8 
                 0.9 
                 1.3 
                 1.3 
                 1.0 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + A174T + G182* + D183* + 
                 1.7 
                 1.7 
                 1.2 
                 1.1 
                 2.4 
                 1.9 
                 1.8 
                 1.3 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + R116W + A174S + G182* + 
                 1.7 
                 1.6 
                 1.2 
                 1.1 
                 1.8 
                 1.8 
                 1.6 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 Q169E + Q172K + G182* + D183* + N195F 
                 1.7 
                 1.4 
                 1.3 
                 1.0 
                 4.9 
                 1.6 
                 1.8 
                 1.3 
               
               
                 G182* + D183* + A186D 
                 1.7 
                 1.6 
                 1.0 
                 1.0 
                 1.6 
                 1.7 
                 1.3 
                 1.1 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.5 
                 1.1 
                 1.0 
                 1.8 
                 1.6 
                 1.4 
                 1.1 
               
               
                 N195F + V206L + V238A + K391A + G476K 
               
               
                 G182* + D183* + A186N 
                 1.7 
                 1.8 
                 1.2 
                 1.1 
                 3.8 
                 1.8 
                 1.6 
                 1.5 
               
               
                 H1* + N54S + V56T + G109A + A174S + V206L + G182* + 
                 1.7 
                 1.1 
                 1.1 
                 1.0 
                 3.3 
                 1.1 
                 1.3 
                 1.1 
               
               
                 D183* + K391A + G476K 
               
               
                 H1* + N54S + V56T + Q169E + Q172K + A174* + G182* + 
                 1.7 
                 1.4 
                 1.3 
                 1.0 
                 3.6 
                 1.4 
                 1.8 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.7 
                 1.2 
                 1.0 
                 1.7 
                 1.7 
                 1.6 
                 1.3 
               
               
                 N195F + V206F + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + G182* + D183* + N195F + 
                 1.7 
                 1.4 
                 1.1 
                 1.0 
                 1.9 
                 1.6 
                 1.2 
                 1.0 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172K + A174S + G182* + 
                 1.7 
                 1.5 
                 1.1 
                 1.1 
                 3.6 
                 1.6 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q172K + A174* + G182* + 
                 1.6 
                 1.4 
                 1.1 
                 1.1 
                 1.6 
                 1.4 
                 1.3 
                 1.1 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174N + G182* + D183* + 
                 1.6 
                 1.8 
                 1.2 
                 1.2 
                 4.6 
                 2.0 
                 2.0 
                 1.7 
               
               
                 N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + A186N + G182* + 
                 1.6 
                 1.8 
                 1.1 
                 1.0 
                 1.5 
                 1.9 
                 1.7 
                 1.3 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.5 
                 1.0 
                 1.0 
                 1.7 
                 1.7 
                 1.4 
                 1.2 
               
               
                 N195F + V206L + F289L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.2 
                 1.0 
                 0.8 
                 1.2 
                 1.2 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + Y295F + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.2 
                 1.1 
                 1.0 
                 1.2 
                 1.0 
                 1.2 
                 1.0 
               
               
                 N195F + V206L + V291A + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.5 
                 1.4 
                 1.1 
                 1.0 
                 2.1 
                 1.5 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + S244Q + K391A + G476K 
               
               
                 G182* + D183* + F328L 
                 1.5 
                 1.5 
                 1.1 
                 1.1 
                 1.8 
                 1.8 
                 1.6 
                 1.3 
               
               
                 G182* + D183* + N195F + A288V 
                 1.5 
                 1.3 
                 1.2 
                 1.0 
                 1.8 
                 1.6 
                 1.5 
                 1.1 
               
               
                 G182* + D183* + A186N + N195F 
                 1.5 
                 1.6 
                 0.9 
                 0.9 
                 2.4 
                 1.7 
                 1.5 
                 1.2 
               
               
                 G182* + D183* + E190P + N195F 
                 1.5 
                 1.4 
                 0.9 
                 1.0 
                 2.1 
                 1.7 
                 1.1 
                 1.1 
               
               
                 W140Y + G182* + D183* + N195F 
                 1.5 
                 1.4 
                 1.1 
                 1.1 
                 1.4 
                 1.7 
                 1.5 
                 1.3 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.5 
                 1.0 
                 1.1 
                 0.9 
                 1.5 
                 1.0 
                 1.5 
                 1.0 
               
               
                 N195F + V206L + A225V + K391A + G476K 
               
               
                 G182* + D183* + A186D + N195F 
                 1.5 
                 1.7 
                 1.4 
                 1.1 
                 3.3 
                 2.0 
                 1.8 
                 1.6 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.5 
                 1.2 
                 1.1 
                 1.0 
                 1.5 
                 1.2 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + P473R + G476K 
               
               
                 H1* + W48Y + N54S + V56T + G109A + A174S + G182* + 
                 1.5 
                 1.3 
                 1.0 
                 1.1 
                 1.2 
                 1.4 
                 1.3 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + 
                 1.5 
                 1.3 
                 1.4 
                 1.1 
                 3.8 
                 1.4 
                 1.5 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 G182* + D183* + N195F + V238A 
                 1.5 
                 1.5 
                 1.0 
                 1.1 
                 1.9 
                 1.4 
                 1.1 
                 1.0 
               
               
                 G182* + D183* + Y295N 
                 1.4 
                 1.1 
                 1.3 
                 1.0 
                 2.5 
                 1.2 
                 1.6 
                 1.2 
               
               
                 Q172G + G182* + D183* 
                 1.4 
                 1.4 
                 1.1 
                 1.0 
                 1.4 
                 1.3 
                 1.5 
                 1.3 
               
               
                 F113Q + G182* + D183* + N195F 
                 1.4 
                 0.8 
                 1.0 
                 0.8 
                 1.0 
                 1.0 
                 1.3 
                 1.0 
               
               
                 H1* + N54S + V56T + Q98A + G109A + A174S + G182* + 
                 1.4 
                 1.3 
                 1.2 
                 1.0 
                 2.7 
                 1.1 
                 1.5 
                 1.2 
               
               
                 D183* + N195F + V206L + K391A + G476K 
               
               
                 G182* + D183* + N195F + Y295F 
                 1.3 
                 1.3 
                 1.2 
                 1.1 
                 1.3 
                 1.2 
                 1.4 
                 1.1 
               
               
                 G182* + D183* + A186E 
                 1.3 
                 1.2 
                 1.2 
                 1.0 
                 2.0 
                 1.4 
                 1.4 
                 1.2 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.3 
                 1.1 
                 1.2 
                 1.1 
                 2.8 
                 1.2 
                 1.3 
                 1.2 
               
               
                 N195F + V206Y + K391A + G476K 
               
               
                 G182* + D183* + N195F + W284H 
                 1.3 
                 1.6 
                 1.1 
                 1.2 
                 3.3 
                 1.8 
                 1.6 
                 1.6 
               
               
                 G182* + D183* + A186Q + N195F 
                 1.3 
                 1.2 
                 1.1 
                 1.0 
                 2.0 
                 1.7 
                 1.5 
                 1.1 
               
               
                 K72R + G182* + D183* 
                 1.3 
                 1.3 
                 1.0 
                 1.0 
                 1.4 
                 1.5 
                 1.3 
                 1.1 
               
               
                 D183* + G182* + N195F + V326L 
                 1.3 
                 1.6 
                 1.2 
                 1.2 
                 3.5 
                 1.8 
                 1.5 
                 1.6 
               
               
                 D183* + G182* + A186E + N195F 
                 1.3 
                 1.3 
                 1.1 
                 1.0 
                 1.7 
                 1.4 
                 1.5 
                 1.2 
               
               
                 Q169E + G182* + D183* + N195F 
                 1.2 
                 1.0 
                 1.1 
                 1.0 
                 1.7 
                 0.8 
                 1.4 
                 1.2 
               
               
                 D183* + G182* + N195F + K242Q 
                 1.2 
                 0.8 
                 0.9 
                 0.9 
                 2.0 
                 1.2 
                 0.9 
                 1.0 
               
               
                 G7A + G182* + D183* + N195F 
                 1.2 
                 1.2 
                 1.1 
                 1.0 
                 1.5 
                 1.6 
                 1.4 
                 1.1 
               
               
                 D183* + G182* + N195F + N260G 
                 1.2 
                 1.4 
                 1.0 
                 0.9 
                 1.6 
                 1.2 
                 1.3 
                 1.1 
               
               
                 D183* + G182* + R321A + S323N 
                 1.2 
                 1.3 
                 1.1 
                 1.1 
                 1.2 
                 1.1 
                 1.5 
                 1.2 
               
               
                 D183* + G182* + W284H 
                 1.2 
                 0.9 
                 1.2 
                 1.0 
                 0.9 
                 1.0 
                 1.1 
                 1.1 
               
               
                 H1* + D183* + G182* 
                 1.2 
                 1.5 
                 1.0 
                 1.0 
                 3.6 
                 1.7 
                 1.6 
                 1.3 
               
               
                 D183* + G182* + A186H + N195F 
                 1.1 
                 1.3 
                 1.1 
                 1.0 
                 2.0 
                 1.2 
                 1.4 
                 1.0 
               
               
                 D183* + G182* + F289V 
                 1.1 
                 1.3 
                 1.0 
                 1.0 
                 2.2 
                 1.3 
                 1.7 
                 1.4 
               
               
                 D183* + G182* + K391A 
                 1.1 
                 0.7 
                 1.0 
                 0.8 
                 0.7 
                 0.7 
                 1.0 
                 0.8 
               
               
                 D183* + G182* + S304R 
                 1.1 
                 1.2 
                 0.9 
                 0.9 
                 0.6 
                 0.9 
                 1.0 
                 1.0 
               
               
                 W167F + G182* + D183* + N195F 
                 1.1 
                 1.0 
                 1.0 
                 0.9 
                 3.3 
                 1.1 
                 1.3 
                 1.1 
               
               
                 F113Q + R116H + D183* + G182* 
                 1.1 
                 0.7 
                 1.0 
                 0.8 
                 1.2 
                 0.8 
                 1.3 
                 0.9 
               
               
                 D183* + G182* + N270G 
                 1.1 
                 1.0 
                 0.9 
                 0.9 
                 1.0 
                 1.3 
                 1.0 
                 1.0 
               
               
                 D183* + G182* + S244Q 
                 1.0 
                 1.1 
                 1.1 
                 0.9 
                 1.8 
                 1.6 
                 1.5 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.0 
                 1.0 
                 1.1 
                 1.0 
                 0.6 
                 0.6 
                 0.9 
                 0.9 
               
               
                 G184T + N195F + V206L + K391A + G476K 
               
               
                 D183* + G182* + N195F + Y295N 
                 1.0 
                 1.1 
                 1.0 
                 1.0 
                 1.6 
                 0.9 
                 1.2 
                 1.3 
               
               
                 W48F + D183* + G182* 
                 1.0 
                 0.9 
                 1.0 
                 0.9 
                 1.8 
                 1.0 
                 1.3 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.0 
                 1.2 
                 1.0 
                 1.0 
                 1.8 
                 1.1 
                 1.2 
                 1.1 
               
               
                 N195F + V206L + G255A + K391A + G476K 
               
               
                 D183* + G182* + A186Q 
                 1.0 
                 0.7 
                 1.1 
                 0.9 
                 0.4 
                 0.5 
                 1.0 
                 0.9 
               
               
                 W140Y + D183* + G182* 
                 0.9 
                 0.6 
                 0.9 
                 0.9 
                 0.5 
                 0.9 
                 1.1 
                 1.0 
               
               
                 D183* + G182* + K242Q 
                 0.9 
                 0.8 
                 1.0 
                 0.9 
                 0.8 
                 0.9 
                 1.0 
                 1.1 
               
               
                 D183* + G182* + V206L 
                 0.9 
                 0.8 
                 0.9 
                 0.9 
                 1.1 
                 0.9 
                 0.9 
                 0.9 
               
               
                 D183* + G182* + N195F + V206F 
                 0.9 
                 0.9 
                 0.9 
                 1.1 
                 0.5 
                 0.8 
                 1.1 
                 1.0 
               
               
                 Q169E + D183* + G182* 
                 0.9 
                 0.8 
                 1.0 
                 0.9 
                 1.1 
                 0.8 
                 0.9 
                 0.9 
               
               
                 D183* + G182* + N195F + R320A 
                 0.8 
                 0.9 
                 1.1 
                 1.0 
                 0.8 
                 0.9 
                 1.0 
                 1.0 
               
               
                 G182* + D183* + G184T + N195F 
                 0.8 
                 1.1 
                 0.9 
                 1.0 
                 0.9 
                 1.0 
                 1.2 
                 1.0 
               
               
                 D183* + G182* + G184T 
                 0.8 
                 0.9 
                 1.0 
                 0.9 
                 0.7 
                 0.8 
                 1.0 
                 1.1 
               
               
                 N54S + V56T + D183* + G182* 
                 0.8 
                 0.8 
                 1.1 
                 1.0 
                 0.9 
                 0.7 
                 1.1 
                 0.9 
               
               
                 D183* + G182* + P473R 
                 0.8 
                 0.9 
                 1.0 
                 1.0 
                 1.1 
                 0.8 
                 1.0 
                 1.0 
               
               
                 W48Y + D183* + G182* 
                 0.8 
                 0.9 
                 1.1 
                 1.1 
                 1.0 
                 0.6 
                 1.0 
                 0.9 
               
               
                 D183* + G182* + R323A 
                 0.8 
                 0.8 
                 0.9 
                 1.0 
                 1.1 
                 0.8 
                 1.1 
                 0.8 
               
               
                 G7A + D183* + G182* 
                 0.8 
                 0.8 
                 1.1 
                 0.9 
                 1.3 
                 1.1 
                 1.2 
                 1.0 
               
               
                 G109A + D183* + G182* 
                 0.8 
                 0.8 
                 0.8 
                 0.8 
                 0.0 
                 0.6 
                 1.0 
                 1.1 
               
               
                 W167F + D183* + G182* 
                 0.7 
                 0.7 
                 1.0 
                 0.9 
                 1.4 
                 0.9 
                 1.1 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174S + 
                 0.7 
                 0.7 
                 0.8 
                 0.9 
                 1.1 
                 0.5 
                 0.8 
                 0.8 
               
               
                 G182* + D183* + N195F + V206L + K391A + G476K 
               
               
                 A174S + D183* + G182* 
                 0.7 
                 1.2 
                 0.8 
                 0.9 
                 2.2 
                 0.9 
                 1.1 
                 1.3 
               
               
                 R116Q + D183* + G182* 
                 0.7 
                 0.7 
                 0.9 
                 0.9 
                 0.7 
                 0.6 
                 1.1 
                 0.9 
               
               
                 D183* + G182* + N195F + V291A 
                 0.7 
                 0.9 
                 1.0 
                 1.1 
                 0.6 
                 0.9 
                 0.9 
                 1.0 
               
               
                 Q172K + D183* + G182* 
                 0.7 
                 0.9 
                 0.9 
                 1.0 
                 0.4 
                 0.5 
                 1.1 
                 0.9 
               
               
                 N54S + D183* + G182* 
                 0.7 
                 1.2 
                 1.0 
                 1.1 
                 1.7 
                 1.1 
                 1.0 
                 1.3 
               
               
                 D183* + G182* + N195F + S323N 
                 0.7 
                 0.9 
                 0.8 
                 0.9 
                 1.8 
                 1.0 
                 1.0 
                 1.0 
               
               
                 D183* + G182* + N195F + M246V 
                 0.7 
                 0.8 
                 1.1 
                 1.0 
                 0.6 
                 0.5 
                 0.9 
                 0.9 
               
               
                 W48Y + D183* + G182* + N195F 
                 0.6 
                 1.2 
                 1.1 
                 1.1 
                 2.0 
                 1.5 
                 1.2 
                 1.4 
               
               
                 D183* + G182* + N195F + S304R 
                 0.6 
                 1.1 
                 0.7 
                 1.0 
                 1.6 
                 0.7 
                 1.0 
                 1.2 
               
               
                 V56T + D183* + G182* 
                 0.3 
                 0.8 
                 0.2 
                 0.6 
                 1.9 
                 0.6 
                 0.3 
                 0.7 
               
               
                   
               
               
                   
                 A-15- 
                 A-15- 
                 A-40- 
                 A-40- 
                 J-15- 
                 J-15- 
                 J-30- 
                 J-30- 
               
               
                   
                 0.05 
                 0.2 
                 0.05 
                 0.2 
                 0.05 
                 0.2 
                 0.05 
                 0.2 
               
               
                 Modifications in SEQ ID NO: 14 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
               
               
                   
               
               
                 Reference 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
               
               
                 G182* + D183* + N195F 
                 1.4 
                 1.2 
                 1.0 
                 1.0 
                 1.2 
                 1.3 
                 1.1 
                 1.1 
               
               
                 H1* + G182* + D183* + N195F 
                 1.1 
                 1.0 
                 1.0 
                 1.1 
                 1.1 
                 1.0 
                 1.1 
                 1.1 
               
               
                 N54S + G182* + D183* + N195F 
                 1.2 
                 1.1 
                 0.9 
                 1.1 
                 1.3 
                 1.1 
                 1.1 
                 1.1 
               
               
                 V56T + G182* + D183* + N195F 
                 1.4 
                 1.1 
                 1.0 
                 1.0 
                 1.5 
                 1.2 
                 1.1 
                 1.1 
               
               
                 G182* + D183* + N195F + I405L 
                 1.1 
                 1.1 
                 1.0 
                 1.0 
                 1.1 
                 1.1 
                 1.0 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.0 
                 1.1 
                 0.9 
                 0.9 
                 1.4 
                 1.0 
                 1.1 
                 0.9 
               
               
                 N195F + V206L + I405L + A421H + A422P + A428T + 
               
               
                 D476K 
               
               
                 A174S + G182* + D183* + N195F 
                 1.2 
                 1.0 
                 1.0 
                 0.9 
                 0.9 
                 0.9 
                 1.0 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.4 
                 1.3 
                 1.1 
                 1.0 
                 1.6 
                 1.3 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + A174S + D183* + N195F + 
                 1.2 
                 1.3 
                 1.0 
                 1.0 
                 1.6 
                 1.3 
                 1.2 
                 1.1 
               
               
                 V206L + K391A + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.3 
                 1.3 
                 1.1 
                 1.0 
                 1.4 
                 1.3 
                 1.1 
                 1.0 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + D476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.2 
                 1.1 
                 1.1 
                 1.0 
                 1.4 
                 1.2 
                 1.2 
                 1.1 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + D476G 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.3 
                 1.3 
                 1.0 
                 1.0 
                 1.5 
                 1.2 
                 1.1 
                 1.0 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + D476K + G477S 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.3 
                 1.2 
                 1.0 
                 1.0 
                 1.5 
                 1.2 
                 1.2 
                 1.0 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + D476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + W167F + A174S + 
                 1.1 
                 1.2 
                 1.0 
                 1.1 
                 1.4 
                 1.4 
                 1.3 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + I405L + A421H + 
               
               
                 A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 0.9 
                 1.2 
                 1.1 
                 0.9 
                 1.3 
                 1.2 
                 1.3 
                 1.0 
               
               
                 N195F + V206L + S280Q + H321Y + I405L + A421H + 
               
               
                 A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.1 
                 1.2 
                 1.0 
                 1.0 
                 1.2 
                 1.1 
                 1.1 
                 1.0 
               
               
                 N195F + V206L + S304N + I405L + A421H + A422P + 
               
               
                 A428T + D476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.2 
                 1.2 
                 1.0 
                 1.0 
                 1.2 
                 1.0 
                 1.0 
                 0.9 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + D476K + G477A 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.6 
                 1.4 
                 1.1 
                 1.1 
                 1.9 
                 1.4 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + D476G + G477T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116Q + Q172N + 
                 0.9 
                 1.2 
                 0.8 
                 1.1 
                 0.8 
                 1.7 
                 0.9 
                 1.1 
               
               
                 A174S + G182* + D183* + N195F + V206L + I405L + 
               
               
                 A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.7 
                 1.3 
                 1.1 
                 1.0 
                 1.9 
                 1.4 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + G477Q 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.5 
                 1.4 
                 1.1 
                 1.0 
                 1.9 
                 1.4 
                 1.3 
                 1.1 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + G477A 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.3 
                 1.3 
                 1.0 
                 1.0 
                 1.7 
                 1.2 
                 1.1 
                 1.0 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + D476K + G477Q 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.5 
                 1.3 
                 1.1 
                 1.1 
                 1.7 
                 1.4 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + D476N 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.5 
                 1.3 
                 1.1 
                 1.0 
                 1.7 
                 1.3 
                 1.3 
                 1.2 
               
               
                 N195F + V206L + K391A + I405L + A421H + A422P + 
               
               
                 A428T + D476Y 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + 
                 1.5 
                 1.2 
                 1.1 
                 1.1 
                 2.2 
                 1.4 
                 1.4 
                 1.1 
               
               
                 N195F + V206I + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + Q172D + A174S + G182* + 
                 1.4 
                 1.3 
                 1.1 
                 1.0 
                 2.8 
                 2.0 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + A204G + V206L + I405L + A421H + 
               
               
                 A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + A174S + 
                 1.3 
                 1.1 
                 1.0 
                 1.0 
                 2.1 
                 2.1 
                 1.3 
                 1.0 
               
               
                 G182* + D183* + N195F + A204G + V206L + G255A + 
               
               
                 I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + T165G + 
                 1.3 
                 1.3 
                 1.0 
                 1.0 
                 2.9 
                 1.9 
                 1.3 
                 1.1 
               
               
                 Q172G + A174S + G182* + D183* + N195F + V206L + 
               
               
                 I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + Q172N + A174S + 
                 1.3 
                 1.3 
                 1.1 
                 1.1 
                 1.9 
                 2.2 
                 1.5 
                 1.3 
               
               
                 G182* + D183* + N195F + V206L + I405L + A421H + 
               
               
                 A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + A174S + 
                 1.4 
                 1.3 
                 1.1 
                 1.1 
                 2.5 
                 2.3 
                 1.5 
                 1.3 
               
               
                 G182* + D183* + N195F + G196R + V206L + G255A + 
               
               
                 I405L + A421H + A422P + A428T + N475S 
               
               
                 H1* + N54S + V56T + G109A + T134E + A174S + G182* + 
                 1.8 
                 1.5 
                 1.2 
                 1.0 
                 2.3 
                 1.8 
                 1.5 
                 1.2 
               
               
                 D183* + N195F + V206L + I405L + A421H + A422P + 
               
               
                 A428T 
               
               
                 H1* + T40D + N54S + V56T + G109A + A174S + G182* + 
                 2.0 
                 1.5 
                 1.2 
                 1.1 
                 1.8 
                 1.6 
                 1.5 
                 1.3 
               
               
                 D183* + N195F + V206L + I405L + A421H + A422P + 
               
               
                 A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + N125D + A174S + 
                 2.1 
                 1.6 
                 1.3 
                 1.0 
                 2.2 
                 1.7 
                 1.5 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + I405L + A421H + 
               
               
                 A422P + A428T 
               
               
                 H1* + N54S + V56T + A60V + G109A + R116Q + A174S + 
                 1.6 
                 1.6 
                 1.1 
                 1.0 
                 1.9 
                 1.6 
                 1.4 
                 1.2 
               
               
                 G182* + D183* + N195F + V206L + I405L + A421H + 
               
               
                 A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + R116V + A174S + G182* + 
                 1.4 
                 1.4 
                 1.0 
                 1.0 
                 2.1 
                 1.4 
                 1.4 
                 1.2 
               
               
                 D183* + N195F + V206L + I405L + A421H + A422P + 
               
               
                 A428T 
               
               
                 H1* + N54S + V56T + G109A + R116Q + G133Q + T165G + 
                 1.1 
                 1.4 
                 1.0 
                 1.1 
                 2.0 
                 2.3 
                 1.5 
                 1.3 
               
               
                 Q172G + A174S + G182* + D183* + N195F + V206L + 
               
               
                 I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + Q172G + 
                 1.7 
                 1.6 
                 1.2 
                 1.1 
                 3.0 
                 1.9 
                 1.5 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + I405L + 
               
               
                 A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116Q + Q172G + 
                 1.4 
                 1.5 
                 1.1 
                 1.1 
                 2.1 
                 1.8 
                 1.3 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + I405L + 
               
               
                 A421H + A422P + A428T + G448D 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + Q172N + 
                 2.2 
                 1.6 
                 1.3 
                 1.2 
                 2.5 
                 1.6 
                 1.6 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + I405L + 
               
               
                 A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + T134E + W167F + 
                 2.2 
                 1.6 
                 1.3 
                 1.2 
                 2.4 
                 1.6 
                 1.7 
                 1.2 
               
               
                 Q172N + A174S + G182* + D183* + N195F + V206L + 
               
               
                 G255A + A265G + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + R116Q + W167F + 
                 2.0 
                 1.6 
                 1.2 
                 1.2 
                 2.3 
                 1.6 
                 1.7 
                 1.2 
               
               
                 Q172R + L173V + A174S + G182* + D183* + N195F + 
               
               
                 V206L + A265G + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + R116Q + W167F + 
                 2.1 
                 1.4 
                 1.3 
                 1.2 
                 2.3 
                 1.6 
                 1.7 
                 1.2 
               
               
                 Q172R + A174S + G182* + D183* + N195F + V206L + 
               
               
                 I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + T134E + 
                 2.0 
                 1.5 
                 1.1 
                 1.1 
                 2.6 
                 1.9 
                 1.4 
                 1.2 
               
               
                 W167F + Q172G + A174S + G182* + D183* + N195F + 
               
               
                 V206L + G255A + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116H + 
                 1.7 
                 1.6 
                 1.0 
                 0.9 
                 1.8 
                 1.5 
                 1.1 
                 1.1 
               
               
                 W167F + Q172G + L173V + A174S + G182* + D183* + 
               
               
                 G184T + N195F + V206L + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + W167F + Q172G + 
                 1.2 
                 1.2 
                 1.0 
                 0.9 
                 2.2 
                 1.3 
                 1.2 
                 1.1 
               
               
                 A174S + G182* + D183* + N195F + V206L + I405L + 
               
               
                 A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + 
                 1.5 
                 1.2 
                 1.2 
                 1.0 
                 2.2 
                 1.1 
                 1.5 
                 1.1 
               
               
                 D183* + N195F + V206L + G255A + I405L + A421H + 
               
               
                 A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116Q + A174S + 
                 1.6 
                 1.3 
                 1.1 
                 1.0 
                 1.4 
                 1.2 
                 1.3 
                 1.1 
               
               
                 G182* + D183* + N195F + V206L + I405L + A421H + 
               
               
                 A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + F116H + 
                 1.8 
                 1.6 
                 1.0 
                 1.0 
                 0.9 
                 1.4 
                 0.9 
                 1.1 
               
               
                 W167F + Q172G + L173V + A174S + G182* + D183* + 
               
               
                 N195F + V206L + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + R116Q + 
                 1.8 
                 1.5 
                 1.1 
                 1.0 
                 1.9 
                 1.5 
                 1.3 
                 1.1 
               
               
                 W167F + Q172G + A174S + G182* + D183* + G184T + 
               
               
                 N195F + V206L + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116Q + W167F + 
                 2.0 
                 1.5 
                 1.0 
                 1.0 
                 1.8 
                 1.5 
                 1.2 
                 1.0 
               
               
                 Q172G + A174S + G182* + D183* + N195F + A204G + 
               
               
                 V206L + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + W167F + 
                 1.5 
                 1.3 
                 0.9 
                 0.9 
                 1.4 
                 1.3 
                 1.2 
                 1.1 
               
               
                 Q172G + A174S + G182* + D183* + N195F + V206L + 
               
               
                 A265G + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + R116Q + A174S + G182* + 
                 1.8 
                 1.6 
                 1.0 
                 0.9 
                 1.6 
                 1.3 
                 1.4 
                 1.1 
               
               
                 D183* + N195F + V206L + I405L + A421H + A422P + 
               
               
                 A428T 
               
               
                 H1* + N54S + V56T + A60V + G109A + R116Q + W167F + 
                 1.4 
                 1.4 
                 0.7 
                 0.9 
                 1.1 
                 1.3 
                 0.8 
                 1.0 
               
               
                 Q172N + L173V + A174S + G182* + D183* + N195F + 
               
               
                 V206L + I405L + A421H + A422P + A428T 
               
               
                 H1* + A37H + N54S + V56T + A60V + G109A + R116Q + 
                 1.9 
                 1.6 
                 1.1 
                 1.1 
                 1.7 
                 1.6 
                 1.4 
                 1.2 
               
               
                 T165G + A174S + G182* + D183* + N195F + V206L + 
               
               
                 I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + A174S + M105F + G182* + 
                 1.4 
                 1.2 
                 1.0 
                 1.0 
                 1.5 
                 1.2 
                 1.2 
                 1.0 
               
               
                 D183* + N195F + V206L + L228I + R320A + S323N + 
               
               
                 I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + F113Q + T134E + 
                 1.8 
                 1.5 
                 1.1 
                 1.0 
                 1.7 
                 2.1 
                 1.4 
                 1.2 
               
               
                 A174S + G182* + D183* + N195F + V206L + G255A + 
               
               
                 I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + K72R + G109A + R116Q + V120L + 
                 2.1 
                 1.8 
                 1.2 
                 1.1 
                 2.8 
                 2.5 
                 1.6 
                 1.3 
               
               
                 A174S + G182* + D183* + G184T + N195F + V206L + 
               
               
                 I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116H + W167F + 
                 2.6 
                 1.9 
                 1.2 
                 1.1 
                 2.8 
                 2.6 
                 1.6 
                 1.3 
               
               
                 Q172G + L173V + A174S + G182* + D183* + N195F + 
               
               
                 V206L + I405L + A421H + A422P + A428T 
               
               
                 H1* + N54S + V56T + G109A + F113Q + R116Q + Q172N + 
                 2.3 
                 1.6 
                 1.1 
                 1.1 
                 3.0 
                 2.1 
                 1.5 
                 1.3 
               
               
                 A174S + G182* + D183* + N195F + V206L + A265G + 
               
               
                 I405L + A421H + A422P + A428T 
               
               
                 H1* + A37H + N54S + V56T + A60V + G109A + R116Q + 
                 2.1 
                 1.7 
                 1.2 
                 1.1 
                 2.5 
                 2.1 
                 1.5 
                 1.2 
               
               
                 W167F + Q172R + A174S + G182* + D183* + N195F + 
               
               
                 V206L + I405L + A421H + A422P + A428T 
               
               
                   
               
            
           
         
       
     
     Example 4: Wash Performance of a Subset of Variants According to the Invention (AMSA) 
     The following variants have been tested as described above. The color measurements were done by use of a professional flatbed scanner (Kodak iQsmart, Kodak) used to capture an image of the washed textile and with a controlled digital imaging system (DigiEye) for capture an image of the washed melamine plates. 
     To extract a value for the light intensity from the scanned images, 24-bit pixel values from the image are converted into values for red, green and blue (RGB). The intensity value (Int) is calculated by adding the RGB values together as vectors and then taking the length of the resulting vector: 
     
       
         
           
             Int 
             = 
             
               
                 
                   r 
                   2 
                 
                 + 
                 
                   g 
                   2 
                 
                 + 
                 
                   b 
                   2 
                 
               
             
           
         
       
     
     The results obtained were the following; 
     
       
         
           
               
               
               
               
               
               
               
               
               
             
               
                   
               
               
                   
                 A-15- 
                 A-15- 
                 A-40- 
                 A-40- 
                 J-15- 
                 J-15- 
                 J-30- 
                 J-30- 
               
               
                   
                 0.05 
                 0.2 
                 0.05 
                 0.02 
                 0.05 
                 0.2 
                 0.05 
                 0.2 
               
               
                 Modifications in SEQ ID NO: 13 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
                 mg/L 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 Reference 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
               
               
                 G182* + D183* + N195F + V206F + N260P + L312I + L313I + R320A + T355L + 
                 0.7 
                 1.0 
                 0.6 
                 0.7 
                 1.3 
                 0.9 
                 0.8 
                 1.0 
               
               
                 L389I + T400P 
               
               
                 G132* + D183* + N195F 
                 0.1 
                 0.7 
                 1.0 
                 1.0 
                 1.0 
                 0.8 
                 1.0 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + K391A + 
                 1.7 
                 1.9 
                 1.7 
                 1.3 
                 2.5 
                 1.9 
                 1.2 
                 1.2 
               
               
                 G476K 
               
               
                 H1* + G50A + T51A + V56T + G109A + A174S + G182* + D183* + N195F + V206L + 
                 1.5 
                 1.6 
                 1.5 
                 1.4 
                 2.5 
                 1.6 
                 1.3 
                 1.2 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + M105F + G109A + A174S + G182* + D183* + N195F + V206L + 
                 2.5 
                 2.2 
                 1.6 
                 1.4 
                 3.8 
                 1.9 
                 1.2 
                 1.1 
               
               
                 R320A + S323N + K391A + G476K 
               
               
                 H1* + T40K + N54S + V56T + G109A + G149H + A174S + G182* + D183* + N195F + 
                 0.9 
                 1.2 
                 1.0 
                 1.0 
                 0.8 
                 1.2 
                 0.9 
                 1.1 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + K72R + G109A + A174S + G182* + D183* + N195F + V206L + 
                 1.9 
                 1.7 
                 1.4 
                 1.4 
                 2.8 
                 1.5 
                 1.2 
                 1.1 
               
               
                 G255A + K391A + G476K 
               
               
                 H1* + N16H + V17L + N54S + V56T + G109A + A174S + G182* + D183* + N195F + 
                 2.6 
                 1.8 
                 1.7 
                 1.4 
                 3.6 
                 1.8 
                 1.3 
                 1.1 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + Q22N + N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + 
                 2.5 
                 1.9 
                 1.8 
                 1.4 
                 3.8 
                 2.1 
                 1.3 
                 1.1 
               
               
                 K391A + G476K 
               
               
                 H1* + Q32S + N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + 
                 2.3 
                 1.9 
                 1.9 
                 1.5 
                 3.3 
                 2.0 
                 1.2 
                 1.2 
               
               
                 K391A + G476K 
               
               
                 H1* + H28N + N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + 
                 2.7 
                 1.7 
                 1.6 
                 1.5 
                 4.2 
                 2.0 
                 1.3 
                 1.1 
               
               
                 K391A + G476K 
               
               
                 H1* + N29S + N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + 
                 1.6 
                 1.3 
                 1.3 
                 1.4 
                 2.6 
                 1.6 
                 1.2 
                 1.0 
               
               
                 K391A + G476K 
               
               
                 H1* + H28N + N29S + N54S + V56T + G109A + A174S + G182* + D183* + N195F + 
                 1.8 
                 1.9 
                 1.8 
                 1.4 
                 3.1 
                 1.8 
                 1.2 
                 1.1 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + R176K + N195F + V206L + K391A + G476K 
                 2.3 
                 2.0 
                 1.7 
                 1.5 
                 5.1 
                 1.9 
                 1.3 
                 1.2 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + R176K + N195F + V206L + 
                 3.8 
                 2.4 
                 2.1 
                 1.7 
                 5.6 
                 2.8 
                 1.4 
                 1.2 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + N175G + N195F + V206L + K391A + G476K 
                 2.6 
                 1.8 
                 1.8 
                 1.4 
                 4.9 
                 2.0 
                 1.3 
                 1.2 
               
               
                 H1* + N54S + V56T + G109A + A174S + N195F + V206L + V291A + Y295N + Q299T + 
                 1.7 
                 1.4 
                 1.4 
                 1.3 
                 2.7 
                 1.6 
                 1.0 
                 1.1 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + N195F + V206L + K269S + A274K + K391A + 
                 1.7 
                 1.4 
                 1.6 
                 1.2 
                 1.4 
                 1.8 
                 1.0 
                 1.0 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + N195F + V206L + L235I + K391A + G476K 
                 2.6 
                 1.5 
                 2.0 
                 1.3 
                 1.3 
                 2.1 
                 1.0 
                 1.1 
               
               
                 H1* + N54S + V56T + G109A + A174S + N195F + V206L + L235V + V238A + K391A + 
                 2.8 
                 1.4 
                 1.7 
                 1.2 
                 0.6 
                 2.2 
                 1.1 
                 1.0 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + N195F + V206L + Q365S + K391A + G476K 
                 1.9 
                 1.5 
                 1.9 
                 1.3 
                 2.0 
                 1.7 
                 1.2 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + A174S + N195F + V206L + K391A + H402R + G476K 
                 2.2 
                 1.4 
                 2.0 
                 1.2 
                 0.6 
                 1.2 
                 1.1 
                 1.1 
               
               
                 H1* + N54S + V56T + G109A + A174S + N19SF + V206L + R320S + H321N + K391A + 
                 5.6 
                 2.7 
                 2.5 
                 1.6 
                 4.2 
                 2.9 
                 1.2 
                 1.2 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N195F + V206L + K391A + 
                 3.8 
                 2.6 
                 3.0 
                 1.7 
                 3.6 
                 2.8 
                 1.3 
                 1.2 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q125S + A174S + N195F + V206L + K391A + G476K 
                 3.1 
                 2.2 
                 2.4 
                 1.4 
                 2.0 
                 2.3 
                 1.3 
                 1.1 
               
               
                 H1* + N54S + V56T + G109A + A174S + N19SF + V206L + L235V + K391A + G476K 
                 4.2 
                 1.9 
                 1.9 
                 1.5 
                 1.8 
                 2.4 
                 1.0 
                 1.0 
               
               
                 H1* + N54S + V56T + G109A + A174S + N195F + V206L + A265G + K391A + G476K 
                 4.2 
                 1.5 
                 2.2 
                 1.4 
                 1.2 
                 2.6 
                 1.1 
                 1.1 
               
               
                 H1* + T51Q + N54S + V56T + G109A + A174S + N195F + Y203F + V206L + K391A + 
                 3.7 
                 2.1 
                 2.6 
                 1.6 
                 3.6 
                 2.7 
                 1.3 
                 1.1 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q169E + Q172K + A174* + N175Q + N195F + V206L + 
                 4.0 
                 2.0 
                 2.7 
                 1.5 
                 3.3 
                 2.8 
                 1.2 
                 1.2 
               
               
                 K391A + G476K 
               
               
                 H1* + H28N + N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + 
                 4.5 
                 2.3 
                 2.5 
                 1.6 
                 3.1 
                 2.7 
                 1.2 
                 1.1 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + T134E + A174S + G182* + D183* + N195F + V206L + 
                 3.9 
                 2.1 
                 2.6 
                 1.6 
                 3.3 
                 3.0 
                 1.4 
                 1.1 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + D377H + 
                 5.3 
                 2.9 
                 3.0 
                 1.7 
                 3.8 
                 2.9 
                 1.3 
                 1.2 
               
               
                 K391A + G476K 
               
               
                 H1* + G50A + T51A + N54S + V56T + G109A + A174S + G182* + D183* + N195F + 
                 4.4 
                 2.1 
                 2.3 
                 1.4 
                 2.6 
                 2.3 
                 1.3 
                 1.2 
               
               
                 V206L + K391A + G476K 
               
               
                 H1* + G7K + N54S + V56T + G109A + A174S + G182* + D183* + N195F + V206L + 
                 4.1 
                 2.3 
                 2.7 
                 1.6 
                 2.4 
                 2.5 
                 1.3 
                 1.2 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + G182* + D183* + N195F + M202V + V206L + 
                 2.7 
                 1.9 
                 1.4 
                 1.4 
                 1.9 
                 2.2 
                 0.9 
                 1.0 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + M105F + G109A + A174S + N195F + V206L + M246F + K391A + 
                 3.7 
                 2.1 
                 2.4 
                 1.5 
                 2.7 
                 2.5 
                 1.2 
                 1.1 
               
               
                 G476K 
               
               
                 H1* + N16H + V17L + N54S + V56T + G109A + A174S + G182* + D183* + N195F + 
                 4.5 
                 2.7 
                 1.8 
                 1.4 
                 4.3 
                 2.2 
                 1.5 
                 1.3 
               
               
                 V206L + D377H + K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + N195F + V206L 
                 1.1 
                 1.9 
                 1.3 
                 1.1 
                 3.0 
                 1.9 
                 1.3 
                 1.1 
               
               
                 H1* + T51G + N54S + V56T + G109A + A174S + N195F + Y203G + V206L + K391A + 
                 2.2 
                 1.8 
                 1.1 
                 1.0 
                 2.7 
                 2.0 
                 0.9 
                 1.1 
               
               
                 G476K 
               
               
                 H1* + T51G + N54S + V56T + G109A + G110N + A174S + N195F + V206L + K391A + 
                 1.6 
                 2.1 
                 1.0 
                 1.1 
                 1.9 
                 1.9 
                 1.1 
                 1.1 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + Q125S + A174S + N175G + N195F + V206L + K391A + 
                 2.0 
                 2.3 
                 1.5 
                 1.2 
                 2.6 
                 1.8 
                 1.3 
                 1.2 
               
               
                 G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + N195F + V206L + A265G + V291A + F328L + 
                 0.7 
                 2.1 
                 1.1 
                 1.0 
                 1.8 
                 1.7 
                 1.0 
                 1.1 
               
               
                 K391A + G476K 
               
               
                 H1* + N54S + V56T + G109A + A174S + N195F + V206L + M246F + A265G + V291A + 
                 3.9 
                 2.6 
                 1.9 
                 1.4 
                 4.0 
                 2.1 
                 1.5 
                 1.2 
               
               
                 F328L + Q365S + K391A + G476K 
               
               
                 H1* + N16Y + N54S + V56T + G109A + R116H + A174S + N195F + V206L + G337E + 
                 4.8 
                 3.5 
                 2.0 
                 1.5 
                 4.9 
                 2.7 
                 1.5 
                 1.3 
               
               
                 D377H + K391A + G476K 
               
               
                 H1* + I9L + N54S + V56T + G109A + Q125S + V131T + A174* + V206L + Y267F + 
                 3.1 
                 2.9 
                 1.4 
                 1.2 
                 2.5 
                 2.0 
                 1.2 
                 1.1 
               
               
                 F289H + Q361E + K391A + G476K 
               
               
                   
               
            
           
         
       
     
     The invention described and claimed herein is not to be limited in scope by the specific aspects herein disclosed, since these aspects are intended as illustrations of several aspects of the invention. Any equivalent aspects are intended to be within the scope of this invention. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims. In the case of conflict, the present disclosure including definitions will control.