Patent Publication Number: US-2021171630-A1

Title: Bispecific CD16-Binding Molecules and Their Use in the Treatment of Disease

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application claims priority to U.S. Patent Appln. Ser. No. 62/597,800 (filed on Dec. 12, 2017; pending), which application is herein incorporated by reference in its entirety. 
     REFERENCE TO SEQUENCE LISTING 
     This application includes one or more Sequence Listings pursuant to 37 C.F.R. 1.821 et seq., which are disclosed in computer-readable media (file name: 1301_0153PCT_Sequence_Listing_ST25.txt, created on Dec. 4, 2018, and having a size of 276,384 bytes), which file is herein incorporated by reference in its entirety. 
     FIELD OF THE INVENTION 
     The present invention is directed to molecules (e.g., an antibody, a diabody, an scFv, an antibody, a TandAb, etc.) capable of binding an epitope of human CD16 (a “CD16 Binding Molecule”). The present invention is further directed to CD16 Binding Molecules that are capable of binding an epitope of human CD16 and one or more epitope(s) of a Disease Antigen (“DA”) (e.g., a “CD16 x DA Binding Molecule”). The present invention is particularly directed to such CD16 x DA Binding Molecules that are antibodies, or that comprise an Epitope Binding Domain thereof, or are diabodies (including DART® diabodies), bispecific antibodies, TandAbs, other multispecific binding molecules (e.g., trivalent TRIDENT™ molecules), etc. The invention particularly concerns CD16 x DA Binding Molecules that are capable of binding a Disease Antigen that is a Cancer Antigen or a Pathogen-Associated Antigen in addition to being able to bind CD16. The invention particularly concerns the use of such CD16 and CD16 x DA Binding Molecules in the treatment of cancer and pathogen-associated diseases. The present invention is also directed to pharmaceutical compositions that comprise such molecule(s). 
    
    
     BACKGROUND OF THE INVENTION 
     The mammalian immune system serves as a defense against a variety of conditions, including, e.g., injury, infection and neoplasia. The efficiency with which humans and other mammals develop an immunological response to pathogens, foreign substances and cancer antigens rests on two characteristics: the exquisite specificity of the immune response for antigen recognition, and the immunological memory that allows for faster and more vigorous responses upon re-activation with the same antigen (Portolés, P. et al. (2009) “ The TCR/CD 3  Complex: Opening the Gate to Successful Vaccination ,” Current Pharmaceutical Design 15:3290-3300; Guy, C. S. et al. (2009) “ Organization of Proximal Signal Initiation at the TCR:CD 3  Complex ,” Immunol Rev. 232(1):7-21; Topalian, S. L. et al. (2015) “ Immune Checkpoint Blockade: A Common Denominator Approach to Cancer Therapy ,” Cancer Cell 27:450-461). 
     In healthy individuals, the immune system is in a quiescent state, inhibited by a repertoire of diverse inhibitory receptors and receptor ligands. Upon recognition of a cancer antigen, microbial pathogen, or an allergen, an array of activating receptors and receptor ligands are triggered to induce the activation of the immune system. Such activation leads to the activation of macrophages, Natural Killer (NK) cells and antigen-specific, cytotoxic, T-cells, and promotes the release of various cytokines, all of which act to counter the perceived threat to the health of the subject (Dong, C. et al. (2003) “ Immune Regulation by Novel Costimulatory Molecules ,” Immunolog. Res. 28(1):39-48; Viglietta, V. et al. (2007) “ Modulating Co - Stimulation ,” Neurotherapeutics 4:666-675; Korman, A. J. et al. (2007) “ Checkpoint Blockade in Cancer Immunotherapy ,” Adv. Immunol. 90:297-339). The immune system is capable of returning to its normal quiescent state when the countervailing inhibitory immune signals outweigh the activating immune signals. 
     Thus, the disease state of cancer (and indeed the disease states of infectious diseases) may be considered to reflect a failure to adequately activate a subject&#39;s immune system. Such failure may reflect an inadequate presentation of activating immune signals, or it may reflect an inadequate ability to alleviate inhibitory immune signals in the subject. In some instances, researchers have determined that cancer cells can co-opt the immune system to evade being detected by the immune system (Topalian, S. L. et al. (2015) “Immune Checkpoint Blockade:  A Common Denominator Approach to Cancer Therapy ,” Cancer Cell 27:450-461). 
     Among the receptors involved in the activation of the immune system are the Fc Receptors:CD16, CD32 and CD64. These Fc receptors are found on the surfaces of multiple types of immune system cells (e.g., B lymphocytes, follicular dendritic cells, natural killer cells, macrophages, neutrophils, eosinophils, basophils and mast cells). Such receptors have an “extracellular” portion (which is thus capable of ligating to an Fc Domain), a “transmembrane” portion (which extends through the cellular membrane), and a “cytoplasmic” portion (positioned inside the cell). Multiple types of FcγRs have been identified: CD16A (FcγRIIIA), CD16B (FcγRIIIB), CD32A (FcγRIIA), CD32B (FcγRIIB), and CD64 (FcγRI). These receptors bind the Fc portion of IgG antibodies, thereby triggering the transduction of activating or inhibitory signals to the immune system. 
     CD16 is a generic name for the activating Fc receptors, FcγRIIIA (CD16A) and FcγRIIIB (CD16B). These receptors bind the Fc portion of IgG antibodies, thereby triggering the release of cytokines. If such antibodies are bound to a Disease Antigen that is expressed on the surface of a cell (e.g., a cancer cell, pathogen-infected cell, etc.), then such release mediates the killing of the targeted cell. 
     CD16A is expressed by Natural Killer (NK) cells and tissue macrophages that bind aggregated but not monomeric human IgG (Selvaraj, P. et al. (2004) “ Functional Regulation Of Human Neutrophil Fc Gamma Receptors ,” Immunol Res. 29(1-3):219-230; Peltz, G. A. et al. (1989) “ Human Fc Gamma RIII: Cloning, Expression, And Identification Of The Chromosomal Locus Of Two Fc Receptors For IgG ,” Proc. Natl. Acad. Sci. (U.S.A.) 86(3):1013-1017; Bachanova, V. et al. (2014) “ NK Cells In Therapy Of Cancer ,” Crit. Rev. Oncog. 19(1-2): 133-141; Miller, J. S. (2013) “ Therapeutic Applications: Natural Killer Cells In The Clinic ,” Hematology Am. Soc. Hematol. Educ. Program. 2013:247-253; Youinou, P. et al. (2002) “ Pathogenic Effects Of Anti - Fc Gamma Receptor IIIB  ( CD 16)  On Polymorphonuclear Neutrophils In Non - Organ - Specific Autoimmune Diseases ,” Autoimmun Rev. 1(1-2):13-19; Peipp, M. et al. (2002) “ Bispecific Antibodies Targeting Cancer Cells ,” Biochem. Soc. Trans. 30(4):507-511; Unkeless, J. C. et al. (1995) “ Function Of Human Fc Gamma RIIA And Fc Gamma RIIIB ,” Semin. Immunol. 7(1):37-44). 
     The expression of CD16A by Natural Killer (NK) cells has particular relevance to the present invention, since such cells release cytokines when their CD16 molecules bind to the Fc Domain of an antibody. Thus, when a natural antibody binds to a Disease Antigen of a target cell, its Fc Domain can be recognized by a CD16 molecule of a Natural Killer cell, which then mediates the killing of the target cell. Since such killing is antibody-dependent, it is termed antibody-dependent cell-mediated cytotoxicity (ADCC). ADCC thus depends on a prior antibody response, and, as stated, requires the presence and participation of an FcγR-expressing effector cell (typically natural killer (NK) cells, but also macrophages, neutrophils and eosinophils). CD16A signals ADCC through interactions with the CD ξ protein of NK cells, and signals macrophage-mediated killing through interactions with macrophage FcγR chains. 
     CD16A possesses two major polymorphic forms, F158 and V158, which differ by possessing a phenylalanine or a valine at residue 158 (shown as residue 160 of the extracellular domain of CD16A ( FIG. 7 ), which corresponds to residue 176 of the full-length protein (Wu, J. et al. (1997) “ A Novel Polymorphism of FcγRIIIa  ( CD 16)  Alters Receptor Function and Predisposes to Autoimmune Disease ,” J. Clin.Invest. 100(5):1059-1070; Ravetch, J. V. et al. (1989) “ Alternative Membrane Forms Of FcγRIII ( CD 16)  On Human Natural Killer Cells And Neutrophils ,” J. Exper. Med. 170:481-497; Koene, H. R. et al. (1997) “ FcγRIIIa -158 V/F Polymorphism Influences the Binding of IgG by Natural Killer Cell FcγRIIIa, Independently of the FcγRIIIa -48 L/R/H Phenotype ,” Blood, 90(3):1109-1114; van Sorge, N. M. (2003) “ FcgammaR Polymorphisms: Implications For Function, Disease Susceptibility And Immunotherapy ,” Tissue Antigens 61(3):189-202; Fijen, C. A. (2000) “ The Role Of Fcgamma Receptor Polymorphisms And C 3  In The Immune Defence Against Neisseria Meningitidis In Complement - Deficient Individuals ,” Clin. Exp. Immunol. 120(2):338-345). 50% of Caucasians are homozygous for the phenylalanine polymorphism (F158/F158), whereas 39% of Caucasians are heterozygous for this polymorphism (F158/V158) and 11% of Caucasians are homozygous for the valine polymorphism (V158/V158). 
     CD16B is expressed on neutrophils, and is anchored to glycophosphatidylinositol (“GPI-anchored”) (Meknache, N. et al. (2009) “ Human Basophils Express The Glycosylphosphatidylinositol - Anchored Low Affinity IgG Receptor FcgammaRIIIB  ( CD 16 B ),” J. Immunol. 182(4):2542-2550 Fernandes, M. J. et al. (2006) “ CD 16 b Associates With High - Density, Detergent - Resistant Membranes In Human Neutrophils ,” Biochem. J. 393(Pt 1):351-359; Selvaraj, P. et al. (2004) “ Functional Regulation Of Human Neutrophil Fc Gamma Receptors ,” Immunol Res. 29(1-3):219-230; Unkeless, J. C. et al. (1995) “ Function Of Human Fc Gamma RIIA And Fc Gamma RIIIB ,” Semin. Immunol. 7(1):37-44). Although thought to be a decoy receptor, it can also transmit signals (Fernandes, M. J. (2005) “ Signaling Through CD 16 b In Human Neutrophils Involves The Tec Family Of Tyrosine Kinases ,” J. Leukoc. Biol. 78(2):524-532), and is downregulated/cleaved by ADAM17 after cell activation (Wang, Y. et al. (2013) “ ADAM 17  Cleaves CD 16 b  ( FcγRIIIb )  In Human Neutrophils ,” Biochim. Biophys. Acta 1833(3):680-685; Guo, S. et al. (2012) “ Role of ADAM 10  and ADAM 17  in CD 16 b Shedding Mediated By Different Stimulators ,” Chin. Med. Sci. J. 27(2):73-79). 
     CD16B possesses two major polymorphic forms, NA1 and NA2, which exhibit different binding affinities for IgG1 and IgG3 subclasses (Bournazos, S. et al. (2010) “ Fcγ Receptor IIIb  ( CD 16 b )  Polymorphisms Are Associated With Susceptibility To Idiopathic Pulmonary Fibrosis ,” Lung 188(6):475-481; van Sorge, N. M. (2003) “ FcgammaR Polymorphisms: Implications For Function, Disease Susceptibility And Immunotherapy ,” Tissue Antigens 61(3):189-202). 13% of Caucasians and 16% of Indians are homozygous for the NA1 polymorphism (NA1/NA1), whereas 55% of Caucasians and 28% of Indians are heterozygous for this polymorphism (NA1/NA2) and 32% of Caucasians and 55% of Indians are homozygous for the NA2 polymorphism (NA2/NA2). The alignment of human CD16A and CD16B allotypes is shown in  FIG. 7 . 
     CD32A (FcγRIIA) (Brandsma, A. M. (2015) “ Fc Receptor Inside - Out Signaling And Possible Impact On Antibody Therapy ,” Immunol Rev. 268(1):74-87; van Sorge, N. M. et al. (2003) “ FcgammaR Polymorphisms: Implications For Function, Disease Susceptibility And Immunotherapy ,” Tissue Antigens 61(3):189-202; Selvaraj, P. et al. (2004) “ Functional Regulation Of Human Neutrophil Fc Gamma Receptors ,” Immunol. Res. 29(1-3):219-230) and CD64 (FcγRI) (Lu, S. et al. (2015) “ Structural Mechanism Of High Affinity FcγRI recognition Of Immunoglobulin G ,” Immunol. Rev. 268(1):192-200; Swisher, J. F. et al. (2015) “ The Many Faces Of FcγRI: Implications For Therapeutic Antibody Function ,” Immunol. Rev. 268(1):160-174; Thepen, T. et al. (2009) “ Fcgamma Receptor  1 ( CD 64),  A Target Beyond Cancer ,” Curr. Pharm. Des. 15(23):2712-2718; Rouard, H. et al. (1997) “ Fc Receptors As Targets For Immunotherapy ,” Int. Rev. Immunol. 16(1-2):147-185) are activating Fc receptors that are expressed on macrophages, neutrophils, eosinophils and dendritic cells (and for CD32A, also on platelets and Langerhan cells). In contrast, CD32B (FcγRIIB) is an inhibiting Fc receptor on B lymphocytes (macrophages, neutrophils, and eosinophils) (Stopforth, R. J. et al. (2016) “ Regulation of Monoclonal Antibody Immunotherapy by FcγRIIB ,” J. Clin. Immunol. [2016 Feb. 27 Epub], pp. 1-7; Bruhns, P. et al. (2009) “ Specificity And Affinity Of Human Fcgamma Receptors And Their Polymorphic Variants For Human IgG Subclasses ,” Blood. 113(16):3716-3725; White, A. L. et al. (2014) “FcγRIIB  As A Key Determinant Of Agonistic Antibody Efficacy ,” Curr. Top. Microbiol. Immunol. 382:355-372; Selvaraj, P. et al. (2004) “ Functional Regulation Of Human Neutrophil Fc Gamma Receptors ,” Immunol. Res. 29(1-3):219-230). 
     The ability of the different FcγRs to mediate diametrically opposing functions reflects their structural differences, and in particular whether the FcγR possesses an immunoreceptor tyrosine-based activation motif (“ITAM”) or an immunoreceptor tyrosine-based inhibitory motif (“ITIM”). The recruitment of different cytoplasmic enzymes to these structures dictates the outcome of the FcγR-mediated cellular responses. ITAM-containing FcγRs include FcγRT, FcγRIIA, FcγRIIIA, and activate the immune system when bound to Fc Domains (e.g., aggregated Fc Domains present in an immune complex). FcγRIIB is the only currently known natural ITIM-containing FcγR; it acts to dampen or inhibit the immune system when bound to aggregated Fc Domains. 
     Although natural IgG antibodies directed to an epitope of a particular Disease Antigen possess Fc Domains that can interact with CD16 molecules to activate a subject&#39;s immune response, in the case of many diseases and many subjects, such immune system activation is not sufficient to provide an effective therapy for the disease. Thus, despite prior advances in identifying the molecules involved in mammalian immune responses, a need remains for improved therapies for treating cancers and infectious diseases. The CD16-Binding Molecules of the present invention, and particularly, the CD16 x DA Binding Molecules of the present invention that comprise a CD16 Binding Domain and a Binding Domain specific for a Disease Antigen expressed on a target cell are capable of co-localizing CD16-expressing cells to the site(s) of cells expressing the Disease Antigen. Such co-localization enhances the ADCC-mediated killing of target cells by increasing the likelihood that an Fc portion of an antibody directed against an epitope of the Disease Antigen will bind to a CD16-expressing effector cell and, via such Fc-CD16 interaction, trigger immune system activation and the release of cytokines against the target cell. Thus, the present invention is directed to improving the activation of a subject&#39;s immune response to a Disease antigen and other goals. 
     SUMMARY OF THE INVENTION 
     The present invention is directed to molecules (e.g., an antibody, a diabody, an scFv, an antibody, a TandAb, etc.) capable of binding an epitope of human CD16 (a “CD16 Binding Molecule”). The present invention is further directed to CD16 Binding Molecules that are capable of binding an epitope of human CD16 and one or more epitope(s) of a Disease Antigen (“DA”) (e.g., a “CD16 x DA Binding Molecule”). The present invention is particularly directed to such CD16 x DA Binding Molecules that are antibodies, or that comprise an Epitope Binding Domain thereof, or are diabodies (including DART® diabodies), bispecific antibodies, TandAbs, other multispecific binding molecules (e.g., trivalent TRIDENT™ molecules), etc. The invention particularly concerns CD16 x DA Binding Molecules that are capable of binding a Disease Antigen that is a Cancer Antigen or a Pathogen-Associated Antigen in addition to being able to bind CD16. The invention particularly concerns the use of such CD16 and CD16 x DA Binding Molecules in the treatment of cancer and pathogen-associated diseases. The present invention is also directed to pharmaceutical compositions that comprise such molecule(s). 
     In detail, the invention provides a CD16 x Disease Antigen (CD16 x DA) Binding Molecule comprising a CD16 Binding Domain capable of binding an epitope of CD16 and also a Disease Antigen-Binding Domain capable of binding an epitope of a Disease Antigen, wherein the CD16 Binding Domain comprises one or more of:
     (I) (A) a CDR H 1 Domain comprising the amino acid sequence of SEQ ID NO:66;
       (B) a CDR H 2 Domain comprising the amino acid sequence of SEQ ID NO:67;   (C) a CDR H 3 Domain comprising the amino acid sequence of SEQ ID NO:68 or SEQ ID NO:60;   (D) a CDR L 1 Domain comprising the amino acid sequence of SEQ ID NO:69 or SEQ ID NO:74;   (E) a CDR L 2 Domain comprising the amino acid sequence of SEQ ID NO:70; and   (F) a CDR L 3 Domain comprising the amino acid sequence of SEQ ID NO:71 or SEQ ID NO:61;   
       (II) (A) a CDR H 1 Domain comprising the amino acid sequence of SEQ ID NO:77;
       (B) a CDR H 2 Domain comprising the amino acid sequence of SEQ ID NO:78;   (C) a CDR H 3 Domain comprising the amino acid sequence of SEQ ID NO:79;   (D) a CDR L 1 Domain comprising the amino acid sequence of SEQ ID NO:80;   (E) a CDR L 2 Domain comprising the amino acid sequence of SEQ ID NO:81; and   (F) a CDR L 3 Domain comprising the amino acid sequence of SEQ ID NO:82;   
       (III) a VH Domain comprising the amino acid sequence of SEQ ID NO:72, SEQ ID NO:83, SEQ ID NO:84, or SEQ ID NO:58;   (IV) a VL Domain comprising the amino acid sequence of SEQ ID NO:73, SEQ ID NO:85, or SEQ ID NO:59;   (V) a VH Domain comprising the amino acid sequence of SEQ ID NO:72 and a VL Domain comprising the amino acid sequence of SEQ ID NO:73; and   (VI) a VH Domain comprising the amino acid sequence of SEQ ID NO:83 or SEQ ID NO:84 and a VL Domain comprising the amino acid sequence of SEQ ID NO:85   (VII) a VH Domain comprising the amino acid sequence of SEQ ID NO:58 and a VL Domain comprising the amino acid sequence of SEQ ID NO:73;   (VIII) a VH Domain comprising the amino acid sequence of SEQ ID NO:72 and a VL Domain comprising the amino acid sequence of SEQ ID NO:59; and   (IX) a VH Domain comprising the amino acid sequence of SEQ ID NO:58 and a VL Domain comprising the amino acid sequence of SEQ ID NO:59.   

     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecule, wherein the Molecule is a bispecific antibody, a bispecific diabody, a bispecific TandAb, a bispecific trivalent molecule, or a bispecific CAR. 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the Molecule is capable of binding more than one Disease Antigen and/or more than one epitope of CD16. 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the CD16 Binding Domain comprises:
         (A) a CDR H 1 Domain comprising the amino acid sequence of SEQ ID NO:66;   (B) a CDR H 2 Domain comprising the amino acid sequence of SEQ ID NO:67;   (C) a CDR H 3 Domain comprising the amino acid sequence of SEQ ID NO:68, or SEQ ID NO:60;   (D) a CDR L 1 Domain comprising the amino acid sequence of SEQ ID NO:69 or SEQ ID NO:74;   (E) a CDR L 2 Domain comprising the amino acid sequence of SEQ ID NO:70; and   (F) a CDR L 3 Domain comprising the amino acid sequence of SEQ ID NO:71, or SEQ ID NO:61.       

     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the CD16 Binding Domain comprises:
         (A) a VH Domain comprising the amino acid sequence of SEQ ID NO:72, or SEQ ID NO:58;   (B) a VL Domain comprising the amino acid sequence of SEQ ID NO:73, or SEQ ID NO:59; or   (C) a VH Domain comprising the amino acid sequence of SEQ ID NO:72 and a VL Domain comprising the amino acid sequence of SEQ ID NO:73   (D) a VH Domain comprising the amino acid sequence of SEQ ID NO:58 and a VL Domain comprising the amino acid sequence of SEQ ID NO:73;   (E) a VH Domain comprising the amino acid sequence of SEQ ID NO:72 and a VL Domain comprising the amino acid sequence of SEQ ID NO:59; or   (F) a VH Domain comprising the amino acid sequence of SEQ ID NO:58 and a VL Domain comprising the amino acid sequence of SEQ ID NO:59.       

     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the CD16 Binding Domain comprises:
         (A) a CDR H 1 Domain comprising the amino acid sequence of SEQ ID NO:77;   (B) a CDR H 2 Domain comprising the amino acid sequence of SEQ ID NO:78;   (C) a CDR H 3 Domain comprising the amino acid sequence of SEQ ID NO:79;   (D) a CDR L 1 Domain comprising the amino acid sequence of SEQ ID NO:80;   (E) a CDR L 2 Domain comprising the amino acid sequence of SEQ ID NO:81; and   (F) a CDR L 3 Domain comprising the amino acid sequence of SEQ ID NO:82.       

     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the CD16 Binding Domain comprises:
         (A) a VH Domain comprising the amino acid sequence of SEQ ID NO:83, or SEQ ID NO:84;   (B) a VL Domain comprising the amino acid sequence of or SEQ ID NO:85; or   (C) a VH Domain comprising the amino acid sequence of SEQ ID NO:83 or SEQ ID NO:84 and a VL Domain comprising the amino acid sequence of SEQ ID NO:85.       

     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the Disease Antigen is a Cancer Antigen and the disease is cancer. 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the cancer is selected from the group consisting of adrenal cancer, bladder cancer, breast cancer, colorectal cancer, gastric cancer, glioblastoma, kidney cancer, non-small-cell lung cancer, acute lymphocytic leukemia, acute myeloid leukemia, chronic lymphocytic leukemia, chronic myeloid leukemia, hairy cell leukemia, Burkett&#39;s lymphoma, diffuse large B cell lymphoma, follicular lymphoma, mantle cell lymphoma, marginal zone lymphoma, non-Hodgkin&#39;s lymphoma, small lymphocytic lymphoma, multiple myeloma, melanoma, ovarian cancer, pancreatic cancer, prostate cancer, skin cancer, renal cell carcinoma, testicular cancer, and uterine cancer. 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the Cancer Antigen is selected from the group consisting of the Cancer Antigens: 19.9, 4.2, A33, ADAM-9, AH6, ALCAM, B1, B7-H3, BAGE, beta-catenin, blood group ALe b /Le y , Burkitt&#39;s lymphoma antigen-38.13, C14, CA125, Carboxypeptidase M, CD5, CD19, CD20, CD22, CD23, CD25, CD27, CD28, CD33, CD36, CD40/CD154, CD45, CD56, CD46, CD52, CD56, CD79a/CD79b, CD103, CD123, CD317, CDK4, CEA, CEACAM5/CEACAM6, CO17-1A, CO-43, CO-514, CTA-1, CTLA-4, Cytokeratin 8, D1.1, Di56-22, DR5, E 1  series, EGFR, an Ephrin receptor, EphA2, Erb, GAGE, a GD2/GD3/GM2 ganglioside, GICA 19-9, gp100, Gp37, gp75, gpA33, HER2/neu, HMFG, human papillomavirus-E6/human papillomavirus-E7, HMW-MAA, I antigen, IL13Rα2, Integrin (36, JAM-3, KID3, KID31, KS 1/4 pan-carcinoma antigen, L6,L20, LEA, LUCA-2, M1:22:25:8, M18, M39, MAGE, MART, mesothelin, MUC-1, MUM-1, Myl, N-acetylglucosaminyltransferase, neoglycoprotein, NS-10, OFA-1, OFA-2, Oncostatin M, p15, p97, PEM, PEMA, PIPA, PSA, PSMA, prostatic acid phosphate, R 24 , ROR1, a sphingolipid, SSEA-1, SSEA-3, SSEA-4, sTn, the T cell receptor derived peptide, T 5 A 7 , TAG-72, TL5, TNF-receptor, TNF-γ receptor, TRA-1-85, a Transferrin Receptor, 5T4, TSTA, VEGF, a VEGF Receptor, VEP8, VEP9, VIM-D5, and Y hapten, Le y . 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the Disease Antigen is 5T4, B7-H3, CEACAM5/CEACAM6, CD19, CD123, EGRF, EphA2, HER2/neu, IL13Rα2 or VEGF. 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the Disease Antigen is a Pathogen-Associated Antigen. 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the Pathogen-Associated Antigen is selected from the group consisting of the Pathogen-Associated Antigens: Herpes Simplex Virus infected cell protein (ICP)47, Herpes Simplex Virus gD, Epstein-Barr Virus LMP-1, Epstein-Barr Virus LMP-2A, Epstein-Barr Virus LMP-2B, Human Immunodeficiency Virus gp160, Human Immunodeficiency Virus gp120, Human Immunodeficiency Virus gp41, etc.), Human Papillomavirus E6, Human Papillomavirus E7, human T-cell leukemia virus gp64, human T-cell leukemia virus gp46, and human T-cell leukemia virus gp21. 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the Disease Antigen is an HIV env antigen. 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the molecule is:
         (A) a diabody, said diabody being a covalently bonded complex that comprises two, or three, four or five polypeptide chains; or   (B) a trivalent binding molecule, said trivalent binding molecule being a covalently bonded complex that comprises three, four or five polypeptide chains, or   (C) a bispecific antibody.       

     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the molecule comprises an Fc Region. 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the Fc Region, is of the IgG1, IgG2, IgG3, or IgG4 isotype. 
     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules, wherein the Fc Region is a variant Fc Region that comprises:
         (A) one or more amino acid modifications that reduces the affinity of the variant Fc Region for an FcγR; and/or   (B) one or more amino acid modifications that enhances the serum half-life of the variant Fc Region.       

     The invention additionally concerns the embodiment of such CD16 x Disease Antigen Binding Molecules:
         (A) said modifications that reduces the affinity of the variant Fc Region for an FcγR comprise the substitution of L234A; L235A; or L234A and L235A; and   (B) said modifications that that enhances the serum half-life of the variant Fc Region comprise the substitution of M252Y; M252Y and S254T; M252Y and T256E; M252Y, S254T and T256E; or K288D and H435K, wherein said numbering is that of the EU index as in Kabat.       

     The invention additionally concerns a CD16 Binding Molecule, that comprises:
         (A) a CDR H 1 Domain comprising the amino acid sequence of SEQ ID NO:66;   (B) a CDR H 2 Domain comprising the amino acid sequence of SEQ ID NO:67;   (C) a CDR H 3 Domain comprising the amino acid sequence of SEQ ID NO:68, or SEQ ID NO:60;   (D) a CDR L 1 Domain comprising the amino acid sequence of SEQ ID NO:69 or SEQ ID NO:74;   (E) a CDR L 2 Domain comprising the amino acid sequence of SEQ ID NO:70; and   (F) a CDR L 3 Domain comprising the amino acid sequence of SEQ ID NO:71, or SEQ ID NO:61.       

     The invention additionally concerns the embodiment of such a CD16 Binding Molecule wherein the molecule comprises:
         (A) a VH Domain comprising the amino acid sequence of SEQ ID NO:72, or SEQ ID NO:58;   (B) a VL Domain comprising the amino acid sequence of SEQ ID NO:73, or SEQ ID NO:59;   (C) a VH Domain comprising the amino acid sequence of SEQ ID NO:72 and a VL Domain comprising the amino acid sequence of SEQ ID NO:73; or   (D) a VH Domain comprising the amino acid sequence of SEQ ID NO:58 and a VL Domain comprising the amino acid sequence of SEQ ID NO:73;   (E) a VH Domain comprising the amino acid sequence of SEQ ID NO:72 and a VL Domain comprising the amino acid sequence of SEQ ID NO:59; or   (F) a VH Domain comprising the amino acid sequence of SEQ ID NO:58 and a VL Domain comprising the amino acid sequence of SEQ ID NO:59.       

     The invention additionally concerns a CD16 Binding Molecule that comprises:
         (A) a CDR H 1 Domain comprising the amino acid sequence of SEQ ID NO:77;   (B) a CDR H 2 Domain comprising the amino acid sequence of SEQ ID NO:78;   (C) a CDR H 3 Domain comprising the amino acid sequence of SEQ ID NO:79;   (D) a CDR L 1 Domain comprising the amino acid sequence of SEQ ID NO:80;   (E) a CDR L 2 Domain comprising the amino acid sequence of SEQ ID NO:81; and   (F) a CDR L 3 Domain comprising the amino acid sequence of SEQ ID NO:82.       

     The invention additionally concerns the embodiment of such CD16 Binding Molecule wherein the molecule comprises:
         (A) a VH Domain comprising the amino acid sequence of SEQ ID NO:83 or SEQ ID NO:84;   (B) a VL Domain comprising the amino acid sequence of or SEQ ID NO:85; or (C) a VH Domain comprising the amino acid sequence of SEQ ID NO:83 or SEQ ID NO:84 and a VL Domain comprising the amino acid sequence of SEQ ID NO:85.       

     The invention additionally concerns the embodiment of such CD16 Binding Molecules wherein the molecule is selected from the group consisting of: an antibody, a multispecific antibody, a Fab′ fragment, a F(ab′)2 fragment, a (Fv) fragment, a single-chain (scFv), a single-chain antibody, a disulfide-linked bispecific Fv (sdFv), a diabody, a trivalent binding molecule, and a CAR-T molecule. 
     The invention additionally concerns a pharmaceutical composition that comprises any of the above-described CD16 x Disease Antigen Binding Molecules, or CD16 Binding Molecules, and a pharmaceutically acceptable carrier. 
     The invention additionally concerns the use of the above-described pharmaceutical composition in the treatment of a disease characterized by the expression of the Disease Antigen. 
     The invention additionally concerns a method for the treatment of a disease characterized by the expression of the Disease Antigen, comprising administering to a subject in need thereof a therapeutically effective amount of the above-described pharmaceutical composition. 
     The invention additionally concerns the embodiment of such use or method wherein the CD16 x Disease Antigen Binding Molecule is capable of binding more than one Disease Antigen and/or more than one epitope of CD16. 
     The invention additionally concerns such use or method wherein the CD16 x Disease Antigen Binding Molecule, wherein the Disease Antigen is a Cancer Antigen, and the disease is cancer. 
     The invention additionally concerns such use or method wherein the cancer is selected from the group consisting of adrenal cancer, bladder cancer, breast cancer, colorectal cancer, gastric cancer, glioblastoma, kidney cancer, non-small-cell lung cancer, acute lymphocytic leukemia, acute myeloid leukemia, chronic lymphocytic leukemia, chronic myeloid leukemia, hairy cell leukemia, Burkett&#39;s lymphoma, diffuse large B cell lymphoma, follicular lymphoma, mantle cell lymphoma, marginal zone lymphoma, non-Hodgkin&#39;s lymphoma, small lymphocytic lymphoma, multiple myeloma, melanoma, ovarian cancer, pancreatic cancer, prostate cancer, skin cancer, renal cell carcinoma, testicular cancer, and uterine cancer. 
     The invention additionally concerns such use or method wherein the Cancer Antigen is selected from the group consisting of the Cancer Antigens: 19.9, 4.2, A33, ADAM-9, AH6, ALCAM, B1, B7-H3, BAGE, beta-catenin, blood group ALe b /Le y , Burkitt&#39;s lymphoma antigen-38.13, C14, CA125, Carboxypeptidase M, CD5, CD19, CD20, CD22, CD23, CD25, CD27, CD28, CD33, CD36, CD40/CD154, CD45, CD56, CD46, CD52, CD56, CD79a/CD79b, CD103, CD123, CD317, CDK4, CEA, CEACAM5/CEACAM6, CO17-1A, CO-43, CO-514, CTA-1, CTLA-4, Cytokeratin 8, D1.1, Di56-22, DR5, E 1  series, EGFR, an Ephrin receptor, EphA2, Erb, GAGE, a GD2/GD3/GM2 ganglioside, GICA 19-9, gp100, Gp37, gp75, gpA33, HER2/neu, HMFG, human papillomavirus-E6/human papillomavirus-E7, HMW-MAA, I antigen, IL13Rα2, Integrin (36, JAM-3, KID3, KID31, KS 1/4 pan-carcinoma antigen, L6,L20, LEA, LUCA-2, M1:22:25:8, M18, M39, MAGE, MART, mesothelin, MUC-1, MUM-1, Myl, N-acetylglucosaminyltransferase, neoglycoprotein, NS-10, OFA-1, OFA-2, Oncostatin M, p15, p97, PEM, PEMA, PIPA, PSA, PSMA, prostatic acid phosphate, R 24 , ROR1, a sphingolipid, SSEA-1, SSEA-3, SSEA-4, sTn, the T cell receptor derived peptide, T5A7, TAG-72, TL5, TNF-receptor, TNF-γ receptor, TRA-1-85, a Transferrin Receptor, 5T4, TSTA, VEGF, a VEGF Receptor, VEP8, VEP9, VIM-D5, and Y hapten, Le y . 
     The invention additionally concerns such use or method wherein the Disease Antigen is 5T4, B7-H3, CEACAM5/CEACAM6, CD19, CD123, EGRF, EphA2, HER2/neu, IL13Rα2 or VEGF. 
     The invention additionally concerns such use or method wherein the CD16 x Disease Antigen Binding Molecule, wherein the Disease Antigen is a Pathogen-Associated Antigen. 
     The invention additionally concerns such use or method wherein the Pathogen-Associated Antigen is selected from the group consisting of the Pathogen-Associated Antigens: Herpes Simplex Virus infected cell protein (ICP)47, Herpes Simplex Virus gD, Epstein-Barr Virus LMP-1, Epstein-Barr Virus LMP-2A, Epstein-Barr Virus LMP-2B, Human Immunodeficiency Virus gp160, Human Immunodeficiency Virus gp120, Human Immunodeficiency Virus gp41, etc.), Human Papillomavirus E6, Human Papillomavirus E7, human T-cell leukemia virus gp64, human T-cell leukemia virus gp46, and human T-cell leukemia virus gp21. 
     The invention additionally concerns such use or method wherein the Disease Antigen is an HIV env antigen. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIGS. 1A-1B  provide a schematic of a representative covalently bonded diabody having two epitope-binding sites composed of two polypeptide chains, each having an E-coil or K-coil Heterodimer-Promoting Domain (alternative Heterodimer-Promoting Domains are provided below). A cysteine residue may be present in a linker ( FIG. 1A ) and/or in the Heterodimer-Promoting Domain ( FIG. 1B ). VL and VH Domains that recognize the same epitope are shown using the same shading or fill pattern. The wavy line (VWWV) in this and all of the Figures providing schematic presentations of binding molecule domains represents one or more optional Heterodimer-Promoting Domains, that is/are preferably present. 
         FIG. 2  provides a schematic of a representative covalently bonded diabody molecule having two epitope-binding sites composed of two polypeptide chains, each having a CH2 and CH3 Domain, such that the associated chains form all or part of an Fc Region. VL and VH Domains that recognize the same epitope are shown using the same shading or fill pattern. 
         FIGS. 3A-3E  provide schematics showing representative covalently bonded tetravalent diabodies having four epitope-binding sites composed of two pairs of polypeptide chains (i.e., four polypeptide chains in all). One polypeptide chain of each pair possesses a CH2 and CH3 Domain, such that the associated chains form all or part of an Fc Region. VL and VH Domains that recognize the same epitope are shown using the same shading or fill pattern. The two pairs of polypeptide chains may be same. In such embodiments, wherein the two pairs of polypeptide chains are the same and the VL and VH Domains recognize different epitopes (as shown in  FIGS. 3A-3B ), the resulting molecule possesses four epitope-binding sites and is bispecific and bivalent with respect to each bound epitope. In such embodiments, wherein the VL and VH Domains recognize the same epitope (e.g., the same VL Domain CDRs and the same VH Domain CDRs are used on both chains) the resulting molecule possesses four epitope-binding sites and is monospecific and tetravalent with respect to a single epitope. Alternatively, the two pairs of polypeptides may be different. In such embodiments, wherein the two pairs of polypeptide chains are different and the VL and VH Domains of each pair of polypeptides recognize different epitopes (as shown by the different shading and patterns in  FIG. 3C ), the resulting molecule possesses four epitope-binding sites and is tetraspecific and monovalent with respect to each bound epitope.  FIG. 3A  shows an Fc Region-containing diabody which contains a peptide Heterodimer-Promoting Domain comprising a cysteine residue.  FIG. 3B  shows an Fc Region-containing diabody, which contains E-coil and K-coil Heterodimer-Promoting Domains comprising a cysteine residue and a linker (with an optional cysteine residue).  FIG. 3C , shows an Fc-Region-Containing diabody, which contains antibody CH1 and CL domains.  FIGS. 3D-3E  illustrate how selection of the binding domains shown in  FIG. 3B  can result in a CD16 x DA Binding Molecule having two binding sites specific for an epitope of CD16 and two binding sites specific for an epitope of a DA.  FIGS. 3D-3E  illustrate non-limiting examples of how domains may be selected to yield CD16 x DA Binding Molecules having differing orientations (i.e.,  FIG. 3D  employs, a VL CD16 Domain as the VL1 Domain of the Binding Molecule, a VH CD16 Domain as the VH1 Domain of the Binding Molecule, a VL DA Domain as the VL2 Domain of the Binding Molecule, and a VH DA Domain as the VH2 Domain of the Binding Molecule. In contrast,  FIG. 3E  employs, a VL DA Domain as the VL1 Domain of the Binding Molecule, a VH DA Domain as the VH1 Domain of the Binding Molecule, a VL CD16 Domain as the VL2 Domain of the Binding Molecule, and a VH CD16 Domain as the VH2 Domain of the Binding Molecule). As provided below, the VL/VH binding sites formed by the association of the polypeptide chains may be the same or different so as to permit tetravalent binding that is monospecific, bispecific, trispecific or tetraspecific. 
         FIGS. 4A and 4B  provide schematics of a representative covalently bonded diabody molecule having two epitope-binding sites composed of three polypeptide chains. Two of the polypeptide chains possess a CH2 and CH3 Domain, such that the associated chains form all or part of an Fc Region. The polypeptide chains comprising the VL and VH Domain further comprise a Heterodimer-Promoting Domain. VL and VH Domains that recognize the same epitope are shown using the same shading or fill pattern. 
         FIGS. 5A-5D  provide schematics of a representative covalently bonded Binding Molecule having four epitope-binding sites composed of five polypeptide chains.  FIG. 5A  shows the general structure of such a CD16 x DA Binding Molecule. Two of the polypeptide chains possess a CH2 and CH3 Domain, such that the associated chains form an Fc Region that comprises all or part of an Fc Region. The polypeptide chains comprising the linked VL and VH Domains further comprise a Heterodimer-Promoting Domain. VL and VH Domains that recognize the same epitope are shown using the same shading or fill pattern.  FIG. 5B  shows the structure of an alternative CD16 x DA Binding Molecule in which the variable domains shown in  FIG. 5A  have been selected to yield a resultant CD16 x DA Binding Molecule that possesses two non-diabody type binding domains specific for an illustrative DA, HER2/neu, and two diabody-type binding domains specific for CD16.  FIG. 5C  shows the structure of an alternative CD16 x DA Binding Molecule in which the variable domains shown in  FIG. 5A  have been selected to yield a resultant CD16 x DA Binding Molecule that possesses two non-diabody type binding domains specific for CD16 and two diabody-type binding domains specific for HER2/neu.  FIG. 5D  shows the structure of an alternative CD16 x DA Binding Molecule in which the variable domains shown in  FIG. 5A  have been selected to yield a resultant CD16 x DA Binding Molecule that possesses two non-diabody type binding domains specific for an epitope of CD16, one diabody-type binding domains specific for an epitope of HER2/neu and a second diabody-type binding domain specific for an epitope of CD16. Such CD16 epitopes may be the same or different. As will be appreciated, by proper selection of the binding domains shown in  FIG. 5A , any three of the binding domains could have been selected to bind an epitope of CD16. Likewise, any three of the binding domains could have been selected to bind an epitope of HER2/neu. As provided below, the VL/VH binding sites formed by the association of the polypeptide chains may be the same or different so as to permit tetravalent binding that is monospecific, bispecific, trispecific or tetraspecific. 
         FIGS. 6A-6H  provide schematics of representative Fc Region-containing trivalent binding molecules having three epitope-binding sites.  FIG. 6A  schematically the domains of trivalent binding molecules comprising two diabody-type binding domains and a Fab-type binding domain having different domain orientations in which the diabody-type binding domains are N-terminal or C-terminal to an Fc Region.  FIGS. 6B-6C  show the structure of illustrative non-limiting examples of CD16 x DA Binding Molecules in which the variable domains shown in  FIG. 6A  have been selected to yield a resultant CD16 x DA Binding Molecule that possesses a non-diabody type binding domains specific for CD16, a diabody-type binding domain that is specific for an illustrative DA, HER2/neu, and a second diabody-type binding domain that is specific for CD16.  FIG. 6D  illustrates schematically the domains of trivalent binding molecules comprising two diabody-type binding domains and a Fab-type binding domain having different domain orientations in which the diabody-type binding domains are N-terminal or C-terminal to an Fc Region. The molecules in  FIGS. 6A-6D  comprise four chains.  FIGS. 6E and 6F , respectively, illustrate schematically the domains of trivalent binding molecules comprising two diabody-type binding domains N-terminal to an Fc Region, and a Fab-type binding domain in which the light chain and heavy chain are linked via a polypeptide spacer, or an scFv-type binding domain. The trivalent binding molecules in  FIGS. 6G and 611 , respectively, illustrate schematically the domains of trivalent binding molecules comprising two diabody-type binding domains C-terminal to an Fc Region, and a Fab-type binding domain in which the light chain and heavy chain are linked via a polypeptide spacer, or an scFv-type binding domain. The trivalent binding molecules in  FIGS. 6E-611  comprise three chains. VL and VH Domains that recognize the same epitope are shown using the same shading or fill pattern. 
         FIG. 7  shows an alignment of the extracellular domains (ECD) of the 158F allotype of human CD16A (SEQ ID NO:146), the 158V allotype of human CD16A (SEQ ID NO:147), the NA1 allotype of human CD16B (SEQ ID NO:148), the NA2 allotype of human CD16B (SEQ ID NO:149), and the CD16 of cynomolgus monkey (SEQ ID NO:150). Differences in sequence relative to that of the 158F allotype of human CD16A are shown in underlined boldface. In the portion of the human CD16A molecule presented, the 158F/158V polymorphism is found at position 160. 
         FIG. 8  shows the effect of increasing concentrations of human IgG on the ability of CD16 Binding Domains of LNK16, DJ130c and CD16-M1 to bind CD16. 
         FIGS. 9A-9C  show the ability of CD16 Binding Molecules: DART-F and DART-G to bind to NK cells ( FIG. 9A ; CD16A: na), neutrophils ( FIG. 9B ; CD16B:na, and T Cells ( FIG. 9C ) in whole blood of donor subjects, compared with an HIV x CD3 diabody (as a positive control for T-cell binding via its CD3 Binding Domain), an HIV x RSV diabody (as a negative control for all binding), and an h3G8 x RSV diabody comparator molecule. 
         FIGS. 10A-10B  show the ability of CD16 Binding Molecules: DART-F and DART-G to bind to CD16A-expressing cells of a gated lymphocyte population ( FIG. 10A ) and a gated granulocyte population ( FIG. 10B ), compared with an HIV x RSV diabody (as a negative control for all binding), and an h3G8 x RSV diabody comparator molecule. 
         FIGS. 11A-11B  show the ability of CD16 Binding Domains of CD16-M1 and h3G8 to distinguish glycosylation allotypes NA1 ( FIG. 11A ) and NA2 ( FIG. 11B ). 
         FIG. 12  shows the ability of CD16 Binding Domains of antibody hCD16-M1 (present in DART-C) to preferentially bind to NK cells in whole blood. 
         FIG. 13  presents an alignment of the amino acid sequences of human CD16 (SEQ ID NO:183), cynomolgus monkey CD16 (SEQ ID NO:184) and murine CD16 (SEQ ID NO:185). Differences in sequence relative to that of human CD16 are shown in underlined boldface. 
         FIGS. 14A-14E  show the percentage cytotoxicity resulting from a 24 hour incubation of target cancer cells expressing different levels of HER2/neu and Effector cells (either human PBMC (E:T=30:1) or purified human NK cells (E:T=2:1)) in the presence of the CD16 x HER2/neu Binding Molecules: DART-C (having an hCD16-M1 CD16 Binding Domain), DART-D (having an hCD16-M2 CD16 Binding Domain), DART-1 (having an h3G8 CD16 Binding Domain), a negative control HER2/neu x RSV diabody or a positive h3G8 x RSV diabody.  FIG. 14A : N87 HER2/neu target cells (HER2/neu expression: +++)/NK cells; CD16A allotype 158F/158F;  FIG. 14B : MCF7 HER2/neu target cells (HER2/neu expression: +/−)/NK cells; CD16A allotype 158F/158F;  FIG. 14C : MDA-MB-231 HER2/neu target cells (HER2/neu expression: +/−)/PBMCs; CD16A allotype not assessed;  FIG. 14D : N87 HER2/neu target cells (HER2/neu expression: +++)/PBMCs; CD16A allotype 158F/158V;  FIG. 14E : Hs700T HER2/neu target cells (HER2/neu expression: +/−)/PBMCs; CD16A allotype 158F/158V. 
         FIGS. 15A-15C  show the percentage cytotoxicity resulting from a 24 hour incubation of HEK/D371 target cells expressing subtype HIV Env protein and Effector cells (either human PBMC (E:T=30:1) or purified human NK cells (E:T=3:1)) in the presence of the CD16 x HIV env CD16 Binding Molecules: DART-F (having an hCD16-M1 CD16 Binding Domain), DART-G (having an hCD16-M2 CD16 Binding Domain), or DART-2 (having an h3G8 CD16 Binding Domain).  FIG. 15A : 293HEK D371 target cells/PBMCs; CD16A allotype 158F/158V;  FIG. 15B : 293HEK D371 target cells/PBMCs; CD16A allotype 158F/158F;  FIG. 15C : 293HEK D371 target cells/NK cells; CD16A allotype 158F/158V. 
         FIGS. 16A-16B  show the percentage cytotoxicity of target HEK/D371 cells, which express the HIV env protein, in the presence of Effector cells (either Jurkat/CD16A 158F ( FIG. 16A ) or 158V/NFAT-Luc cells ( FIG. 16B ) upon incubation with DART-X (having an hCD16-M1 CD16 Binding Domain), DART-Y (having an hCD16-M2 CD16 Binding Domain), DART-0 (having an h3G8 CD16 Binding Domain) or DART-3 (a CD16 x RSV diabody having an hCD16-M1 CD16 Binding Domain). 
         FIGS. 17A-17C  show the ability of the optimized CD16 Binding Domains of hCD16-M1A (present in DART-I) hCD16-M1B (present in DART-J), hCD16-M1AB (present in DART-K), to bind to human NK cells ( FIG. 17A ), cynomolgus monkey NK cells ( FIG. 17B ), and rhesus monkey NK cells ( FIG. 17C ) present in PBMC samples as compared to parental CD16 Binding Domain of hCD16-M1 (present in DART-C an and DART-3) and the CD16 Binding Domain of h3G8 (present in DART-1). The HER2/neu x RSV diabody (lacking a CD16 Binding Domain) is included as a negative control. 
         FIGS. 18A-18D  show the ability of the optimized CD16 Binding Domains of hCD16-M1A (present in DART-I) hCD16-M1B (present in DART-J), and hCD16-M1AB (present in DART-K) to mediate redirected cell killing of HER2/neu expressing target cells with both human and cynomolgus monkey Effector cells. Plotted is the cytotoxicity resulting from a 24 hour incubation of JIMT-1-Luc target cancer cells and Effector cells (either human PBMCs ( FIGS. 18A-18B ) or cynomolgus monkey PMBCs ( FIGS. 18C-18D )) (E:T=30:1) in the presence of the CD16 x HER2/neu Binding Molecules: DART-C (having an hCD16-M1 CD16 Binding Domain), DART-I (having an hCD16-M1A CD16 Binding Domain), DART-J (having a hCD16-M1B Binding Domain), DART-K (having a hCD16-M1AB Binding Domain), DART-1 (having an h3G8 CD16 Binding Domain), or a negative control HER2/neu x RSV diabody as measured in an LDH redirected cell killing assay (plotted as percent cytotoxicity,  FIGS. 18A and 18C ) or a LUM redirected cell killing assay (luminescence (LUM) is plotted in relative light units (RLU),  FIGS. 18B and 18D ). 
         FIGS. 19A-19D  show the ability of the optimized CD16 Binding Domains of hCD16-M1B (present in DART-M), and hCD16-M1AB (present in DART-N) to mediate redirected cell killing of CD19 expressing target cells with human Effector cells. Cytotoxicity measured after 24 hour ( FIGS. 19A and 19C ) and 48 hour incubations ( FIGS. 19B and 19D ) of Raji-Luc target cancer cells and human PBMC Effector cells (E:T=30:1) in the presence of the CD16 x CD19 Binding Molecules: DART-L (having an hCD16-M1 CD16 Binding Domain), DART-M (having a hCD16-M1B Binding Domain), DART-N (having a hCD16-M1AB Binding Domain), duvortuxizumab (a positive control CD3 x CD19 diabody), or negative control CD16 x RSV diabodies DART-5 (having an hCD16-M1 CD16 Binding Domain), DART-6 (having a hCD16-M1B Binding Domain), DART-7 (having a hCD16-M1AB Binding Domain), as measured in an LDH redirected cell killing assay (plotted as percent cytotoxicity,  FIGS. 19A and 19B ) or a LUM redirected cell killing assay (LUM is plotted in RLU,  FIGS. 19C and 19D ). 
         FIGS. 20A-20D  show the ability of the optimized CD16 Binding Domains of hCD16-M1B (present in DART-M), and hCD16-M1AB (present in DART-N) to mediate autologous B-cell depletion in vitro both human and cynomolgus monkey PBMC samples. The B-cell counts (CD3 − /CD20 +  cells) after 72 hour ( FIGS. 20A and 20C ), 96 hour ( FIG. 20B ), and 144 hour ( FIG. 20D ) incubations of human ( FIGS. 20A-20B ) or cynomolgus monkey ( FIGS. 20C-20D ) PBMCs presence of the CD16 x CD19 Binding Molecules: DART-L (having an hCD16-M1 CD16 Binding Domain), DART-M (having a hCD16-M1B Binding Domain), DART-N (having a hCD16-M1AB Binding Domain), or negative control CD16 x RSV diabodies DART-5 (having an hCD16-M1 CD16 Binding Domain), DART-6 (having a hCD16-M1B Binding Domain) as measured by flow cytometry. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The present invention is directed to molecules (e.g., an antibody, a diabody, an scFv, an antibody, a TandAb, etc.) capable of binding an epitope of human CD16 (a “CD16 Binding Molecule”). The present invention is further directed to CD16 Binding Molecules that are capable of binding an epitope of human CD16 and one or more epitope(s) of a Disease Antigen (“DA”) (e.g., a “CD16 x DA Binding Molecule”). The present invention is particularly directed to such CD16 x DA Binding Molecules that are antibodies, or that comprise an Epitope Binding Domain thereof, or are diabodies (including DART® diabodies), bispecific antibodies, TandAbs, other multispecific binding molecules (e.g., trivalent TRIDENT™ molecules), etc. The invention particularly concerns CD16 x DA Binding Molecules that are capable of binding a Disease Antigen that is a Cancer Antigen or a Pathogen-Associated Antigen in addition to being able to bind CD16. The invention particularly concerns the use of such CD16 and CD16 x DA Binding Molecules in the treatment of cancer and pathogen-associated diseases. The present invention is also directed to pharmaceutical compositions that comprise such molecule(s). 
     I. Antibodies and Other Binding Molecules 
     A. Antibodies 
     The CD16 x DA Binding Molecules of the present invention may be antibodies, or be derivable from antibodies (e.g., by fragmentation, cleavage, etc. of antibody polypeptides, or from use of the amino acid sequences of antibody molecules or of polynucleotides (or their sequences) that encode such polynucleotides, etc.). 
     Antibodies are immunoglobulin molecules capable of specific binding to a particular domain or moiety or conformation (an “epitope”) of a molecule, such as a carbohydrate, polynucleotide, lipid, polypeptide, etc. An epitope-containing molecule may have immunogenic activity, such that it elicits an antibody production response in an animal; such molecules are termed “antigens.” As used herein, the terms “antibody” and “antibodies” refer to monoclonal antibodies, multispecific antibodies, human antibodies, humanized antibodies, synthetic antibodies, chimeric antibodies, polyclonal antibodies, camelized antibodies, single-chain Fvs (scFv), single-chain antibodies, Fab fragments, F(ab′) fragments, disulfide-linked bispecific Fvs (sdFv), intrabodies, and Epitope Binding Domains of any of the above. Such Immunoglobulin molecules can be of any type (e.g., IgG, IgE, IgM, IgD, IgA and IgY), class (e.g., IgG 1 , IgG 2 , IgG 3 , IgG 4 , IgA 1  and IgA 2 ) or subclass. 
     The term “monoclonal antibody” refers to a homogeneous antibody population wherein the monoclonal antibody is comprised of amino acids (naturally occurring or non-naturally occurring) that are involved in the selective binding of an antigen. Monoclonal antibodies are highly specific, being directed against a single epitope (or antigenic site). The term “monoclonal antibody” encompasses not only intact monoclonal antibodies and full-length monoclonal antibodies, but also fragments thereof (such as Fab, Fab′, F(ab′) 2 , (Fv), single-chain (scFv), mutants thereof, fusion proteins comprising an antibody portion, humanized monoclonal antibodies, chimeric monoclonal antibodies, and any other modified configuration of the immunoglobulin molecule that comprises an antigen recognition site of the required specificity and the ability to bind to an antigen. It is not intended to be limited as regards to the source of the antibody or the manner in which it is made (e.g., by hybridoma, phage selection, recombinant expression, transgenic animals, etc.). The term includes whole immunoglobulins as well as the fragments etc. described above under the definition of “antibody.” Methods of making monoclonal antibodies are known in the art. One method which may be employed is the method of Kohler, G. et al. (1975) “ Continuous Cultures Of Fused Cells Secreting Antibody Of Predefined Specificity ,” Nature 256:495-497 or a modification thereof. Typically, monoclonal antibodies are developed in mice, rats or rabbits. The antibodies are produced by immunizing an animal with an immunogenic amount of cells, cell extracts, or protein preparations that contain the desired epitope. The immunogen can be, but is not limited to, primary cells, cultured cell lines, cancerous cells, proteins, peptides, nucleic acids, or tissue. Cells used for immunization may be cultured for a period of time (e.g., at least 24 hours) prior to their use as an immunogen. Cells may be used as immunogens by themselves or in combination with a non-denaturing adjuvant, such as Ribi (see, e.g., Jennings, V. M. (1995) “ Review of Selected Adjuvants Used in Antibody Production ,” ILAR J. 37(3):119-125). In general, cells should be kept intact and preferably viable when used as immunogens. Intact cells may allow antigens to be better detected than ruptured cells by the immunized animal. Use of denaturing or harsh adjuvants, e.g., Freund&#39;s adjuvant, may rupture cells and therefore is discouraged. The immunogen may be administered multiple times at periodic intervals such as, bi weekly, or weekly, or may be administered in such a way as to maintain viability in the animal (e.g., in a tissue recombinant). Alternatively, existing monoclonal antibodies and any other equivalent antibodies that are immunospecific for a desired pathogenic epitope can be sequenced and produced recombinantly by any means known in the art. In one embodiment, such an antibody is sequenced and the polynucleotide sequence is then cloned into a vector for expression or propagation. The sequence encoding the antibody of interest may be maintained in a vector in a host cell and the host cell can then be expanded and frozen for future use. The polynucleotide sequence of such antibodies may be used for genetic manipulation to generate the monospecific or multispecific (e.g., bispecific, trispecific and tetraspecific) molecules of the invention as well as an affinity optimized, a chimeric antibody, a humanized antibody, and/or a caninized antibody, to improve the affinity, or other characteristics of the antibody as detailed below. 
     Antibodies and the Binding Molecules of the present invention bind epitopes via their Binding Domains in an “immunospecific” manner. As used herein, a molecule is said to bind an epitope of another molecule in an immunospecific manner (or “immunospecifically”) if it binds or associates more frequently, more rapidly, with greater duration and/or with greater affinity with that epitope relative to alternative epitopes. For example, an antibody that immunospecifically binds to a viral epitope is an antibody that binds this viral epitope with greater affinity, avidity, more readily, and/or with greater duration than it immunospecifically binds to other viral epitopes or non-viral epitopes. It is also understood by reading this definition that, for example, an antibody (or moiety or epitope) that immunospecifically binds to a first target may or may not specifically or preferentially bind a second target. As such, “immunospecific binding” does not necessarily require (although it can include) exclusive binding. Generally, but not necessarily, reference to binding means “immunospecific” binding. Natural antibodies are capable of binding to only one epitope species (i.e., they are “monospecific”), although they can immunospecifically bind multiple copies of that species (i.e., exhibiting “bivalency” or “multivalency”). Two molecules are said to be capable of binding one another in a “physiospecific” manner, if such binding exhibits the specificity with which receptors bind their respective ligands. 
     The last few decades have seen a revival of interest in the therapeutic potential of antibodies, and antibodies have become one of the leading classes of biotechnology-derived drugs (Chan, C. E. et al. (2009) “ The Use Of Antibodies In The Treatment Of Infectious Diseases ,” Singapore Med. J. 50(7):663-666). Over 200 antibody-based drugs have been approved for use or are under development. 
     1. General Structural Attributes of Antibodies 
     The basic structural unit of naturally occurring immunoglobulins (e.g., IgG) is a tetramer composed of two shorter “Light Chains” complexed with two longer “Heavy Chains” and is usually expressed as a glycoprotein of about 150,000 Da. Each chain is composed of an amino-terminal (“N-terminal”) portion that comprises a “Variable Domain” and a carboxy-terminal (“C-terminal”) portion that comprises at least one “Constant Domain.” An IgG Light Chain is composed of a single “Light Chain Variable Domain” (“VL”) and a single “Light Chain Constant Domain” (“CL”). Thus, the structure of the light chains of an IgG molecule is n-VL-CL-c (where n and c represent, respectively, the N-terminus and the C-terminus of the polypeptide). An IgG Heavy Chain is composed of a single “Heavy Chain Variable Domain” (“VH”), three “Heavy Chain Constant Domains” (“CH1,” “CH2” and “CH3”), and a “Hinge” Region (“H”), located between the CH1 and CH2 Domains. Thus, the structure of an IgG heavy chain is n-VH—CH1-H—CH2-CH3-c (where n and c represent, respectively, the N-terminus and the C-terminus of the polypeptide). The ability of an intact, unmodified antibody (e.g., an IgG antibody) to bind an epitope of an antigen depends upon the presence and sequences of the Variable Domains. Unless specifically noted, the order of domains of the protein molecules described herein is in the “N-terminal to C-terminal” direction. 
     (a) Constant Domains 
     (i) Light Chain Constant Domain 
     A preferred CL Domain is a human IgG CL Kappa Domain. The amino acid sequence of an exemplary human CL Kappa Domain is (SEQ ID NO:1): 
     
       
         
           
               
               
            
               
                   
                 RTVAAPSVFI FPPSDEQLKS GTASVVCLLN NFYPREAKVQ 
               
               
                   
                   
               
               
                   
                 WKVDNALQSG NSQESVTEQD SKDSTYSLSS TLTLSKADYE 
               
               
                   
                   
               
               
                   
                 KHKVYACEVT HQGLSSPVTK SFNRGEC 
               
            
           
         
       
     
     Alternatively, an exemplary CL Domain is a human IgG CL Lambda Domain. The amino acid sequence of an exemplary human CL Lambda Domain is (SEQ ID NO:2): 
     
       
         
           
               
               
            
               
                   
                 QPKAAPSVTL FPPSSEELQA NKATLVCLIS DFYPGAVTVA 
               
               
                   
                   
               
               
                   
                 WKADSSPVKA GVETTPSKQS NNKYAASSYL SLTPEQWKSH 
               
               
                   
                   
               
               
                   
                 RSYSCQVTHE GSTVEKTVAP TECS 
               
            
           
         
       
     
     (ii) Heavy Chain CH1 Domains 
     The CH1 Domains of the two Heavy Chains of an antibody complex with the antibody&#39;s Light Chain&#39;s “CL” constant region, and are attached to the Heavy Chains CH2 Domains via an intervening Hinge Domain. 
     An exemplary CH1 Domain is a human IgG1 CH1 Domain. The amino acid sequence of an exemplary human IgG1 CH1 Domain is (SEQ ID NO:3): 
     
       
         
           
               
               
            
               
                   
                 ASTKGPSVFP LAPSSKSTSG GTAALGCLVK 
               
               
                   
               
               
                   
                 DYFPEPVTVS WNSGALTSGV HTFPAVLQSS 
               
               
                   
               
               
                   
                 GLYSLSSVVT VPSSSLGTQT YICNVNHKPS 
               
               
                   
               
               
                   
                 NTKVDKRV 
               
            
           
         
       
     
     An exemplary CH1 Domain is a human IgG2 CH1 Domain. The amino acid sequence of an exemplary human IgG2 CH1 Domain is (SEQ ID NO:4): 
     
       
         
           
               
               
            
               
                   
                 ASTKGPSVFP LAPCSRSTSE STAALGCLVK 
               
               
                   
               
               
                   
                 DYFPEPVTVS WNSGALTSGV HTFPAVLQSS 
               
               
                   
               
               
                   
                 GLYSLSSVVT VPSSNFGTQT YTCNVDHKPS 
               
               
                   
               
               
                   
                 NTKVDKTV 
               
            
           
         
       
     
     An exemplary CH1 Domain is a human IgG3 CH1 Domain. The amino acid sequence of an exemplary human IgG3 CH1 Domain is (SEQ ID NO:5): 
     
       
         
           
               
               
            
               
                   
                 ASTKGPSVFP LAPCSRSTSG GTAALGCLVK 
               
               
                   
               
               
                   
                 DYFPEPVTVS WNSGALTSGV HTFPAVLQSS 
               
               
                   
               
               
                   
                 GLYSLSSVVT VPSSSLGTQT YTCNVNHKPS 
               
               
                   
               
               
                   
                 NTKVDKRV 
               
            
           
         
       
     
     An exemplary CH1 Domain is a human IgG4 CH1 Domain. The amino acid sequence of an exemplary human IgG4 CH1 Domain is (SEQ ID NO:6): 
     
       
         
           
               
               
            
               
                   
                 ASTKGPSVFP LAPCSRSTSE STAALGCLVK 
               
               
                   
               
               
                   
                 DYFPEPVTVS WNSGALTSGV HTFPAVLQSS 
               
               
                   
               
               
                   
                 GLYSLSSVVT VPSSSLGTKT YTCNVDHKPS 
               
               
                   
               
               
                   
                 NTKVDKRV 
               
            
           
         
       
     
     (b) Heavy Chain Hinge Regions 
     One exemplary Hinge Domain is a human IgG1 Hinge Domain. The amino acid sequence of an exemplary human IgG1 Hinge Domain is (SEQ ID NO:7): 
     
       
         
           
               
               
            
               
                   
                 EPKSCDKTHTCPPCP. 
               
            
           
         
       
     
     Another exemplary Hinge Domain is a human IgG2 Hinge Domain. The amino acid sequence of an exemplary human IgG2 Hinge Domain is (SEQ ID NO:8): 
     
       
         
           
               
               
            
               
                   
                 ERKCCVECPPCP. 
               
            
           
         
       
     
     Another exemplary Hinge Domain is a human IgG3 Hinge Domain. The amino acid sequence of an exemplary human IgG2 Hinge Domain is (SEQ ID NO:9): 
     
       
         
           
               
               
            
               
                   
                 ELKTPLGDTT HTCPRCPEPK SCDTPPPCPR CPEPKSCDTP 
               
               
                   
               
               
                   
                 PPCPRCPEPK SCDTPPPCPR CP. 
               
            
           
         
       
     
     Another exemplary Hinge Domain is a human IgG4 Hinge Domain. The amino acid sequence of an exemplary human IgG4 Hinge Domain is (SEQ ID NO:10): 
                            ESKYGPPCPSCP.            
As described herein, an IgG4 Hinge Domain may comprise a stabilizing mutation such as the S228P substitution. The amino acid sequence of an exemplary S228P-stabilized human IgG4 Hinge Domain is (SEQ ID NO:11):
 
     
       
         
           
               
               
            
               
                   
                 ESKYGPPCPPCP. 
               
            
           
         
       
     
     (c) Heavy Chain CH2 and CH3 Domains 
     The CH2 and CH3 Domains of the two heavy chains interact to form the “Fc Domain” of IgG antibodies that is recognized by cellular Fc Receptors, including but not limited to Fc gamma Receptors (FcγRs). As used herein, the term “Fc Region” is used to define a C-terminal region of an IgG heavy chain. A portion of an Fc Region (including a portion that encompasses an entire Fc Region) is referred to herein as an “Fc Domain.” An Fc Region is said to be of a particular IgG isotype, class or subclass if its amino acid sequence is most homologous to that isotype relative to other IgG isotypes. In addition to their known uses in diagnostics, antibodies have been shown to be useful as therapeutic agents. 
     The amino acid sequence of the CH2-CH3 Domain of an exemplary human IgG1 is (SEQ ID NO:12): 
                            231      240        250       260        270           APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED                            280        290       300       310           PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH                            320        330        340       350                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT                            360        370        380       390           LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN                            400        410        420       430           YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE                           440      447           ALHNHYTQKS LSLSPG   X                
as numbered by the EU index as set forth in Kabat, wherein  X  is lysine (K) or is absent.
 
     The amino acid sequence of the CH2-CH3 Domain of an exemplary human IgG2 is (SEQ ID NO:13): 
                            231      240        250       260        270           APPVA-GPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED                            280        290        300       310           PEVQFNWYVD GVEVHNAKTK PREEQFNSTF RVVSVLTVVH                            320        330        340       350                 QDWLNGKEYK CKVSNKGLPA PIEKTISKTK GQPREPQVYT                            360        370        380       390           LPPSREEMTK NQVSLTCLVK GFYPSDISVE WESNGQPENN                           400         410        420       430           YKTTPPMLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE                           440      447           ALHNHYTQKS LSLSPG   X                
as numbered by the EU index as set forth in Kabat, wherein  X  is lysine (K) or is absent.
 
     The amino acid sequence of the CH2-CH3 Domain of an exemplary human IgG3 is (SEQ ID NO:14): 
                            231      240        250       260        270           APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED                            280        290        300       310           PEVQFKWYVD GVEVHNAKTK PREEQYNSTF RVVSVLTVLH                            320        330        340       350                 QDWLNGKEYK CKVSNKALPA PIEKTISKTK GQPREPQVYT                            360        370        380       390           LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESSGQPENN                            400        410        420       430           YNTTPPMLDS DGSFFLYSKL TVDKSRWQQG NIFSCSVMHE                            440     447           ALHNRFTQKS LSLSPG   X                
as numbered by the EU index as set forth in Kabat, wherein  X  is lysine (K) or is absent.
 
     The amino acid sequence of the CH2-CH3 Domain of an exemplary human IgG4 is (SEQ ID NO:15): 
                            231      240        250       260        270           APEFLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSQED                            280        290        300       310           PEVQFNWYVD GVEVHNAKTK PREEQFNSTY RVVSVLTVLH                            320        330        340       350                 QDWLNGKEYK CKVSNKGLPS SIEKTISKAK GQPREPQVYT                            360        370        380       390           LPPSQEEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN                            400         410       420       430           YKTTPPVLDS DGSFFLYSRL TVDKSRWQEG NVFSCSVMHE                            440     447           ALHNHYTQKS LSLSLG   X                
as numbered by the EU index as set forth in Kabat, wherein  X  is lysine (K) or is absent.
 
     Throughout the present specification, the numbering of the residues in the constant region of an IgG heavy chain is that of the EU index as in Kabat et al., S EQUENCES OF  P ROTEINS OF  I MMUNOLOGICAL  I NTEREST , 5 th  Ed. Public Health Service, NH1, MD (1991) (“Kabat”), expressly incorporated herein by reference. The term “EU index as in Kabat” refers to the numbering of the constant domains of human IgG1 EU antibody. Amino acids from the Variable Domains of the mature heavy and light chains of immunoglobulins are also designated by the position of an amino acid in the chain. Kabat described numerous amino acid sequences for antibodies, identified an amino acid consensus sequence for each subgroup, and assigned a residue number to each amino acid, and the CDRs are identified as defined by Kabat (it will be understood that CDR H 1 as defined by Chothia, C. &amp; Lesk, A. M. ((1987) “ Canonical Structures For The Hypervariable Regions Of Immunoglobulins ,” J. Mol. Biol. 196:901-917) begins five residues earlier). Kabat&#39;s numbering scheme is extendible to antibodies not included in his compendium by aligning the antibody in question with one of the consensus sequences in Kabat by reference to conserved amino acids. This method for assigning residue numbers has become standard in the field and readily identifies amino acids at equivalent positions in different antibodies, including chimeric or humanized variants. For example, an amino acid at position 50 of a human antibody light chain occupies the equivalent position to an amino acid at position 50 of a mouse antibody light chain. 
     Polymorphisms have been observed at a number of different positions within antibody constant regions (e.g., Fc positions, including but not limited to positions 270, 272, 312, 315, 356, and 358 as numbered by the EU index as set forth in Kabat), and thus slight differences between the presented sequence and sequences in the prior art can exist. Polymorphic forms of human immunoglobulins have been well-characterized. At present, 18 Gm allotypes are known: G1m (1, 2, 3, 17) or G1m (a, x, f, z), G2m (23) or G2m (n), G3m (5, 6, 10, 11, 13, 14, 15, 16, 21, 24, 26, 27, 28) or G3m (b1, c3, b3, b0, b3, b4, s, t, g1, c5, u, v, g5) (Lefranc et al., “ The Human IgG Subclasses: Molecular Analysis Of Structure, Function And Regulation .” Pergamon, Oxford, pp. 43-78 (1990); Lefranc, G. et al., 1979, Hum. Genet.: 50, 199-211). It is specifically contemplated that the antibodies of the present invention may incorporate any allotype, isoallotype, or haplotype of any immunoglobulin gene, and are not limited to the allotype, isoallotype or haplotype of the sequences provided herein. Furthermore, in some expression systems the C-terminal amino acid residue (bolded above) of the CH3 Domain may be post-translationally removed. Accordingly, the C-terminal residue of the CH3 Domain is an optional amino acid residue in the CD16 x DA Binding Molecules of the invention. Specifically encompassed by the instant invention are CD16 x DA Binding Molecules lacking the C-terminal residue of the CH3 Domain. Also specifically encompassed by the instant invention are such constructs comprising the C-terminal lysine residue of the CH3 Domain. 
     (d) Variable Domains 
     The Variable Domains of an IgG molecule consist of three “complementarity determining regions” (“CDRs”), which contain the amino acid residues of the antibody that will be in contact with the epitope, as well as intervening non-CDR segments, referred to as “framework regions” (“FRs”), which, in general maintain the structure and determine the positioning of the CDR loops so as to permit such contacting (although certain framework residues may also contact the epitope). Thus, the VL and VH Domains have the structure n-FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4-c. The amino acid sequences of the CDRs determine whether an antibody will be able to bind to a particular epitope. Interaction of an antibody light chain with an antibody heavy chain and, in particular, interaction of their VL and VH Domains, forms an epitope-binding site of the antibody. 
     Polypeptides that are (or may serve as) the first, second and third CDR of the Light Chain of an antibody are herein respectively designated as: CDR L 1 Domain, CDR L 2 Domain, and CDR L 3 Domain. Similarly, polypeptides that are (or may serve as) the first, second and third CDR of the Heavy Chain of an antibody are herein respectively designated as: CDR H 1 Domain, CDR H 2 Domain, and CDR H 3 Domain. Thus, the terms CDR L 1 Domain, CDR L 2 Domain, CDR L 3 Domain, CDR H 1 Domain, CDR H 2 Domain, and CDR H 3 Domain are directed to polypeptides that when incorporated into a protein cause that protein to be able to bind to a specific epitope regardless of whether such protein is an antibody having light and heavy chains or is a diabody or a single-chain binding molecule (e.g., an scFv, a BiTe, etc.), or is another type of protein. 
     The term “Epitope Binding Domain” denotes a fragment or portion of a binding molecule (or a polypeptide having the amino acid sequence of such a fragment or portion) that contributes to the ability of the binding molecule to immunospecifically bind to an epitope. An Epitope Binding Domain may contain a VL or VH Domain of an antibody, or any 1, 2, 3, 4, or 5 of the CDR Domains of an antibody, or may contain all 6 of the CDR Domains of an antibody and, although capable of immunospecifically binding such epitope, may exhibit an immunospecificity, affinity or selectivity towards such epitope that differs from that of such antibody. An Epitope Binding Domain may contain only part of a CDR, namely the subset of CDR residues required for binding, termed the SDRs (Kim, J. H. et al. (2012) “ Humanization By CDR Grafting And Specificity - Determining Residue Grafting ,” Methods Mol. Biol. 907:237-245; Kim, K. S. et al. (2010) “ Construction Of A Humanized Antibody To Hepatitis B Surface Antigen By Specificity - Determining Residues  ( SDR )-Grafting And De Immunization,” Biochem. Biophys. Res. Commun. 396(2):231-237; Kashmiri, S. V. et al. (2005) “ SDR Grafting—A New Approach To Antibody Humanization ,” Methods 36(1):25-34; Gonzales, N. R. et al. (2004) “ SDR Grafting Of A Murine Antibody Using Multiple Human Germline Templates To Minimize Its Immunogenicity ,” Mol. Immunol. 41:863-872). Preferably, however, an Epitope Binding Domain will contain all 6 of the CDR Domains of such antibody. An Epitope Binding Domain may be a single polypeptide chain (e.g., an scFv), or may comprise two or more polypeptide chains, which may each have an amino terminus and a carboxy terminus (e.g., a diabody, a Fab fragment, an Fab 2  fragment, etc.), and which may be covalently bonded to one another via a disulfide bond. 
     2. Humanization of Antibodies 
     The invention also particularly encompasses Binding Molecules that comprise a VL or VH Domain of an antibody, and preferably both a VL and a VH Domain of an antibody. Preferably, such antibody is a humanized antibody. Monoclonal antibodies are typically prepared in non-human species, such as mouse or rabbit. The Variable and/or Constant Domains of such antibodies may be recognized as immunogens, thus provoking an immune response against them. Such molecules may however be “humanized” by introducing one or more amino acid substitutions in order to render such antibodies more like antibodies produced by humans. thereby reducing or eliminating their immunogenicity. The term “humanized” antibody refers to a chimeric molecule, generally prepared using recombinant techniques, having an epitope-binding site of an immunoglobulin from a non-human species and a remaining immunoglobulin structure of the molecule that is based upon the structure and/or sequence of a human immunoglobulin. The polynucleotide sequence of the variable domains of such antibodies may be used for genetic manipulation to generate such derivatives and to improve the affinity, or other characteristics of such antibodies. Application of this approach to various antibodies has been reported by LoBuglio, A. F. et al. (1989) “ Mouse/Human Chimeric Monoclonal Antibody In Man: Kinetics And Immune Response ,” Proc. Natl. Acad. Sci. (U.S.A.) 86:4220-4224; Sato, K. et al. (1993) Cancer Res 53:851-856. Riechmann, L. et al. (1988) “ Reshaping Human Antibodies for Therapy ,” Nature 332:323-327; Verhoeyen, M. et al. (1988) “ Reshaping Human Antibodies: Grafting An Antilysozyme Activity ,” Science 239:1534-1536; Kettleborough, C. A. et al. (1991) “ Humanization Of A Mouse Monoclonal Antibody By CDR - Grafting: The Importance Of Framework Residues On Loop Conformation ,” Protein Engineering 4:773-3783; Maeda, H. et al. (1991) “ Construction Of Reshaped Human Antibodies With HIV - Neutralizing Activity ,” Human Antibodies Hybridoma 2:124-134; Gorman, S. D. et al. (1991) “ Reshaping A Therapeutic CD 4  Antibody ,” Proc. Natl. Acad. Sci. (U.S.A.) 88:4181-4185; Tempest, P. R. et al. (1991) “ Reshaping A Human Monoclonal Antibody To Inhibit Human Respiratory Syncytial Virus Infection in vivo ,” Bio/Technology 9:266-271; Co, M. S. et al. (1991) “ Humanized Antibodies For Antiviral Therapy ,” Proc. Natl. Acad. Sci. (U.S.A.) 88:2869-2873; Carter, P. et al. (1992) “ Humanization Of An Anti - p 185 her 2  Antibody For Human Cancer Therapy ,” Proc. Natl. Acad. Sci. (U.S.A.) 89:4285-4289; and Co, M. S. et al. (1992) “ Chimeric And Humanized Antibodies With Specificity For The CD 33  Antigen ,” J. Immunol. 148:1149-1154. In some embodiments, humanized antibodies preserve all CDR sequences (for example, a humanized mouse antibody which contains all six CDRs from the mouse antibodies). In other embodiments, humanized antibodies have one or more CDRs (one, two, three, four, five, and/or six) which differ in sequence relative to the original antibody. 
     The general principle in humanizing an antibody involves retaining the basic sequence of the Epitope Binding Domain of the antibody, while swapping the non-human remainder of the antibody with human antibody sequences. There are four general steps to humanize a monoclonal antibody. These are: (1) determining the nucleotide and predicted amino acid sequence of the starting antibody light and heavy variable domains (2) designing the humanized antibody or caninized antibody, i.e., deciding which antibody framework region to use during the humanizing or canonizing process (3) the actual humanizing or caninizing methodologies/techniques and (4) the transfection and expression of the humanized antibody. See, for example, U.S. Pat. Nos. 4,816,567; 5,807,715; 5,866,692; and 6,331,415. 
     A number of humanized antibody molecules comprising an Epitope Binding Domain derived from a non-human immunoglobulin have been described, including chimeric antibodies having rodent or modified rodent Variable Domain and their associated complementarity determining regions (CDRs) fused to human constant domains (see, for example, Winter et al. (1991) “ Man - made Antibodies ,” Nature 349:293-299; Lobuglio et al. (1989) “ Mouse/Human Chimeric Monoclonal Antibody In Man: Kinetics And Immune Response ,” Proc. Natl. Acad. Sci. (U.S.A.) 86:4220-4224 (1989), Shaw et al. (1987) “ Characterization Of A Mouse/Human Chimeric Monoclonal Antibody  (17-1 A )  To A Colon Cancer Tumor Associated Antigen ,” J. Immunol. 138:4534-4538, and Brown et al. (1987) “ Tumor - Specific Genetically Engineered Murine/Human Chimeric Monoclonal Antibody ,” Cancer Res. 47:3577-3583). Other references describe rodent CDRs grafted into a human supporting framework region (FR) prior to fusion with an appropriate human antibody Constant Domain (see, for example, Riechmann, L. et al. (1988) “ Reshaping Human Antibodies for Therapy ,” Nature 332:323-327; Verhoeyen, M. et al. (1988) “ Reshaping Human Antibodies: Grafting An Antilysozyme Activity ,” Science 239:1534-1536; and Jones et al. (1986) “ Replacing The Complementarity - Determining Regions In A Human Antibody With Those From A Mouse ,” Nature 321:522-525). Another reference describes rodent CDRs supported by recombinantly veneered rodent framework regions. See, for example, European Patent Publication No. 519,596. These “humanized” molecules are designed to minimize unwanted immunological response towards rodent anti-human antibody molecules, which limits the duration and effectiveness of therapeutic applications of those moieties in human recipients. Other methods of humanizing antibodies that may also be utilized are disclosed by Daugherty et al. (1991) “ Polymerase Chain Reaction Facilitates The Cloning, CDR - Grafting, And Rapid Expression Of A Murine Monoclonal Antibody Directed Against The CD 18  Component Of Leukocyte Integrins ,” Nucl. Acids Res. 19:2471-2476 and in U.S. Pat. Nos. 6,180,377; 6,054,297; 5,997,867; and 5,866,692. The invention particularly encompasses binding molecules (including antibodies and diabodies) that comprise a VL and/or VH Domain of a “humanized” antibody. 
     Notwithstanding such successes, the production of stable, functional heterodimeric, non-monospecific diabodies optimized for therapeutic use can be further improved by the careful consideration and placement of the domains employed in the polypeptide chains. The present invention is thus directed to the provision of specific polypeptides that are particularly designed to form, via covalent bonding, stable and therapeutically useful heterodimeric diabodies and heterodimeric Fc diabodies that are capable of simultaneously binding CD16 and a Disease Antigen. 
     B. Bispecific Antibodies 
     As indicated above, natural antibodies are capable of binding to only one epitope species, although they can bind multiple copies of that species. The ability of an antibody to bind an epitope of an antigen depends upon the presence and amino acid sequence of the antibody&#39;s VL and VH Domains. Interaction of an antibody&#39;s Light Chain and Heavy Chain and, in particular, interaction of its VL and VH Domains forms one of the two Epitope Binding Domains of a natural antibody, such as an IgG. Natural antibodies are capable of binding only one epitope species (i.e., they are mono-specific), although they can bind multiple copies of that species (i.e., exhibiting bi-valency or multi-valency). 
     The functionality of antibodies can be enhanced by generating multispecific antibody-based molecules that can simultaneously bind two separate and distinct antigens (or different epitopes of the same antigen) and/or by generating antibody-based molecule having higher valency (i.e., more than two Binding Domains) for the same epitope and/or antigen. 
     In order to provide molecules having greater capability than natural antibodies, a wide variety of recombinant bispecific antibody formats have been developed (see, e.g., PCT Publication Nos. WO 2008/003116, WO 2009/132876, WO 2008/003103, WO 2007/146968, WO 2009/018386, WO 2012/009544, WO 2013/070565). Most of such approaches use linker peptides to fuse a further binding domain (e.g. an scFv, VL, VH, etc.) to, or within the antibody core (IgA, IgD, IgE, IgG or IgM), or to fuse multiple antibody binding portions to one another (e.g. two Fab fragments or scFv). Alternative formats use linker peptides to fuse a binding protein (e.g., an scFv, VL, VH, etc.) to a dimerization domain such as the CH2-CH3 Domain or alternative polypeptides (WO 2005/070966, WO 2006/107786A WO 2006/107617A, WO 2007/046893). Typically, such approaches involve compromises and trade-offs. For example, PCT Publication Nos. WO 2013/174873, WO 2011/133886 and WO 2010/136172 disclose that the use of linkers may cause problems in therapeutic settings, and teaches a trispecific antibody in which the CL and CH1 Domains are switched from their respective natural positions and the VL and VH Domains have been diversified (WO 2008/027236; WO 2010/108127) to allow them to bind to more than one antigen. Thus, the molecules disclosed in these documents trade binding specificity for the ability to bind additional antigen species. PCT Publication Nos. WO 2013/163427 and WO 2013/119903 disclose modifying the CH2 Domain to contain a fusion protein adduct comprising a binding domain. The document notes that the CH2 Domain likely plays only a minimal role in mediating effector function. PCT Publication Nos. WO 2010/028797, WO2010028796 and WO 2010/028795 disclose recombinant antibodies whose Fc Regions have been replaced with additional VL and VH Domains, so as to form trivalent binding molecules. PCT Publication Nos. WO 2003/025018 and WO2003012069 disclose recombinant diabodies whose individual chains contain scFv domains. PCT Publication Nos. WO 2013/006544 discloses multi-valent Fab molecules that are synthesized as a single polypeptide chain and then subjected to proteolysis to yield heterodimeric structures. Thus, the molecules disclosed in these documents trade all or some of the capability of mediating effector function for the ability to bind additional antigen species. PCT Publication Nos. WO 2014/022540, WO 2013/003652, WO 2012/162583, WO 2012/156430, WO 2011/086091, WO 2008/024188, WO 2007/024715, WO 2007/075270, WO 1998/002463, WO 1992/022583 and WO 1991/003493 disclose adding additional Binding Domains or functional groups to an antibody or an antibody portion (e.g., adding a diabody to the antibody&#39;s light chain, or adding additional VL and VH Domains to the antibody&#39;s light and heavy chains, or adding a heterologous fusion protein or chaining multiple Fab Domains to one another). Thus, the molecules disclosed in these documents trade native antibody structure for the ability to bind additional antigen species. 
     C. Chimeric Antigen Receptors 
     The binding molecules of the present invention may be Chimeric Antigen Receptors (“CARs”) that comprise a single chain variable fragment (scFv) capable of binding CD16 and a Disease Antigen. As indicated above, scFvs are made by linking Light and Heavy Chain Variable Domains together via a short linking peptide. First-generation CARs typically had the intracellular domain from the CD3 chain, which is the primary transmitter of signals from endogenous TCRs. Second-generation CARs possessed additional intracellular signaling domains from various costimulatory protein receptors (e.g., CD28, 41BB, ICOS, etc.) to the cytoplasmic tail of the CAR in order to provide additional signals to the T-cell. Third-generation CARs combine multiple signaling domains, such as CD3z-CD28-41BB or CD3z-CD28-OX40, in order to further augment potency (Tettamanti, S. et al. (2013) “ Targeting Of Acute Myeloid Leukaemia By Cytokine - Induced Killer Cells Redirected With A Novel CD 123- Specific Chimeric Antigen Receptor ,” Br. J. Haematol. 161:389-401; Gill, S. et al. (2014) “ Efficacy Against Human Acute Myeloid Leukemia And Myeloablation Of Normal Hematopoiesis In A Mouse Model Using Chimeric Antigen Receptor Modified T Cells ,” Blood 123(15): 2343-2354; Mardiros, A. et al. (2013) “ T Cells Expressing CD 123- Specific Chimeric Antigen Receptors Exhibit Specific Cytolytic Effector Functions And Antitumor Effects Against Human Acute Myeloid Leukemia ,” Blood 122:3138-3148; Pizzitola, I. et al. (2014) “ Chimeric Antigen Receptors Against CD 33 /CD 123  Antigens Efficiently Target Primary Acute Myeloid Leukemia Cells in vivo ,” Leukemia doi:10.1038/leu.2014.62. 
     The intracellular domain of the CARs of the present invention is preferably selected from the intracellular domain of any of: 41BB-CD3ζ, b2c-CD3ζ, CD28, CD28-4-1BB-CD3ζ, CD28-CD3ζ, CD28-FcεRIγ, CD28mut-CD3ζ, CD28-OX40-CD3ζ, CD28-OX40-CD3ζ, CD3, CD4-CD3ζ, CD4-FcεRIγ, CD8-CD3ζ, FcεRIγ, FcεRIγCAIX, Heregulin-CD3ζ, IL-13-CD3ζ, or Ly49H-CD3ζ (Tettamanti, S. et al. (2013) “ Targeting Of Acute Myeloid Leukaemia By Cytokine - Induced Killer Cells Redirected With A Novel CD 123- Specific Chimeric Antigen Receptor ,” Br. J. Haematol. 161:389-401; Gill, S. et al. (2014) “ Efficacy Against Human Acute Myeloid Leukemia And Myeloablation Of Normal Hematopoiesis In A Mouse Model Using Chimeric Antigen Receptor Modified T Cells ,” Blood 123(15): 2343-2354; Mardiros, A. et al. (2013) “ T Cells Expressing CD 123- Specific Chimeric Antigen Receptors Exhibit Specific Cytolytic Effector Functions And Antitumor Effects Against Human Acute Myeloid Leukemia ,” Blood 122:3138-3148; Pizzitola, I. et al. (2014) “ Chimeric Antigen Receptors Against CD 33 /CD 123  Antigens Efficiently Target Primary Acute Myeloid Leukemia Cells in vivo ,” Leukemia doi:10.1038/leu.2014.62). 
     II. Bispecific Diabodies 
     The art has additionally noted the capability of producing diabodies that differ from natural antibodies in being capable of binding two or more different epitope species (i.e., exhibiting bispecificity or multispecificity in addition to bi-valency or multi-valency) (see, e.g., Holliger et al. (1993) “′ Diabodies&#39;: Small Bivalent And Bispecific Antibody Fragments ,” Proc. Natl. Acad. Sci. (U.S.A.) 90:6444-6448; US 2004/0058400 (Hollinger et al.); US 2004/0220388 (Mertens et al.); Alt et al. (1999) FEBS Lett. 454(1-2):90-94; Lu, D. et al. (2005) “ A Fully Human Recombinant IgG - Like Bispecific Antibody To Both The Epidermal Growth Factor Receptor And The Insulin - Like Growth Factor Receptor For Enhanced Antitumor Activity ,” J. Biol. Chem. 280(20):19665-19672; WO 02/02781 (Mertens et al.); Olafsen, T. et al. (2004) “ Covalent Disulfide - Linked Anti - CEA Diabody Allows Site - Specific Conjugation And Radiolabeling For Tumor Targeting Applications ,” Protein Eng Des Sel. 17(1):21-27; Wu, A. et al. (2001) “ Multimerization Of A Chimeric Anti - CD 20  Single Chain Fv - Fv Fusion Protein Is Mediated Through Variable Domain Exchange ,” Protein Engineering 14(2):1025-1033; Asano et al. (2004) “ A Diabody For Cancer Immunotherapy And Its Functional Enhancement By Fusion Of Human Fc Domain ,” Abstract 3P-683, J. Biochem. 76(8):992; Takemura, S. et al. (2000) “ Construction Of A Diabody  ( Small Recombinant Bispecific Antibody )  Using A Refolding System ,” Protein Eng. 13(8):583-588; Baeuerle, P. A. et al. (2009) “ Bispecific T cell Engaging Antibodies For Cancer Therapy ,” Cancer Res. 69(12):4941-4944). 
     The design of a diabody is based on the structure of the single-chain Variable Domain fragment (scFv), in which Light and Heavy Chain Variable Domains are linked to one another using a short linking peptide. Bird et al. (1988) (“ Single - Chain Antigen - Binding Proteins ,” Science 242:423-426) describes example of linking peptides which bridge approximately 3.5 nm between the carboxy terminus of one Variable Domain and the amino terminus of the other Variable Domain. Linkers of other sequences have been designed and used (Bird et al. (1988) “ Single - Chain Antigen Binding Proteins ,” Science 242:423-426). Linkers can in turn be modified for additional functions, such as attachment of drugs or attachment to solid supports. The single-chain variants can be produced either recombinantly or synthetically. For synthetic production of scFv, an automated synthesizer can be used. For recombinant production of scFv, a suitable plasmid containing polynucleotide that encodes the scFv can be introduced into a suitable host cell, either eukaryotic, such as yeast, plant, insect or mammalian cells, or prokaryotic, such as  E. coli . Polynucleotides encoding the scFv of interest can be made by routine manipulations such as ligation of polynucleotides. The resultant scFv can be isolated using standard protein purification techniques known in the art. 
     The provision of non-monospecific “diabodies” provides a significant advantage over antibodies: the capacity to co-ligate and co-localize cells that express different epitopes. Bispecific diabodies thus have wide-ranging applications including therapy and immunodiagnosis. Bispecificity allows for great flexibility in the design and engineering of the diabody in various applications, providing enhanced avidity to multimeric antigens, the cross-linking of differing antigens, and directed targeting to specific cell types relying on the presence of both target antigens. Due to their bivalency, low dissociation rates and rapid clearance from the circulation (for diabodies of small size, at or below ˜50 kDa), diabody molecules known in the art have also shown particular use in the field of tumor imaging (Fitzgerald et al. (1997) “ Improved Tumour Targeting By Disulphide Stabilized Diabodies Expressed In Pichia pastoris ,” Protein Eng. 10:1221-1225). 
     The ability to produce bispecific diabodies has led to their use (in “trans”) to co-ligate two cells together, for example, by co-ligating receptors that are present on the surface of different cells (e.g., cross-linking cytotoxic T-cells to target cells, such as cancer cells or pathogen-infected cells, that express a Disease Antigen) (Staerz et al. (1985) “ Hybrid Antibodies Can Target Sites For Attack By T Cells ,” Nature 314:628-631, and Holliger et al. (1996) “ Specific Killing Of Lymphoma Cells By Cytotoxic T - Cells Mediated By A Bispecific Diabody ,” Protein Eng. 9:299-305; Marvin et al. (2005) “ Recombinant Approaches To IgG - Like Bispecific Antibodies ,” Acta Pharmacol. Sin. 26:649-658; Sloan et al. (2015) “ Targeting HIV Reservoir in Infected CD 4  T Cells by Dual - Affinity Re - targeting Molecules  ( DARTs )  that Bind HIV Envelope and Recruit Cytotoxic T Cells ,” PLoS Pathog 11(11): e1005233. doi:10.1371/journal.ppat.1005233)). Alternatively (or additionally), bispecific (or multispecific) diabodies can be used (in “cis”) to co-ligate molecules, such as receptors, etc., that are present on the surface of the same cell. Co-ligation of different cells and/or receptors is useful to modulate effector functions and/or immune cell signaling. Multispecific molecules (e.g., bispecific diabodies) comprising Epitope Binding Domains may be directed to a surface determinant of any immune cell such as CD2, CD3, CD8, CD16, TCR, NKG2D, etc., which are expressed on T lymphocytes, Natural Killer (NK) cells, Antigen-Presenting Cells or other mononuclear cells, or to a surface determinant of a B cell, such as CD19, CD20, CD22, CD30, CD37, CD40, and CD74 (Moore, P. A. et al. (2011) “ Application Of Dual Affinity Retargeting Molecules To Achieve Optimal Redirected T cell Killing Of B - Cell Lymphoma ,” Blood 117(17):4542-4551; Cheson, B. D. et al. (2008) “ Monoclonal Antibody Therapy For B - Cell Non Hodgkin&#39;s Lymphoma ,” N. Engl. J. Med. 359(6):613-626; Castillo, J. et al. (2008) “ Newer Monoclonal Antibodies For Hematological Malignancies ,” Exp. Hematol. 36(7):755-768). In particular, Epitope Binding Domains directed to a cell surface receptor that is present on immune effector cells, are useful in the generation of multispecific binding molecules capable of mediating redirected cell killing. 
     In many studies, diabody binding to effector cell determinants, e.g., Fcγ receptors (FcγR), was also found to activate the effector cell (Holliger et al. (1996) “ Specific Killing Of Lymphoma Cells By Cytotoxic T cells Mediated By A Bispecific Diabody ,” Protein Eng. 9:299-305; Holliger et al. (1999) “ Carcinoembryonic Antigen  ( CEA )-Specific T cell Activation In Colon Carcinoma Induced By Anti- CD 3  x Anti - CEA Bispecific Diabodies And B 7  x Anti - CEA Bispecific Fusion Proteins ,” Cancer Res. 59:2909-2916; WO 2006/113665; WO 2008/157379; WO 2010/080538; WO 2012/018687; WO 2012/162068). Normally, effector cell activation is triggered by the binding of an antigen-bound antibody to an effector cell via an Fc Domain-FcγR interaction; thus, in this regard, diabody molecules may exhibit Ig-like functionality independent of whether they comprise an Fc Domain (e.g., as assayed in any effector function assay known in the art or exemplified herein (e.g., ADCC assay)). By cross-linking tumor and effector cells, the diabody not only brings the effector cell within the proximity of a tumor cell but leads to effective tumor killing (see e.g., Cao et al. (2003) “ Bispecific Antibody Conjugates In Therapeutics ,” Adv. Drug. Deliv. Rev. 55:171-197). 
     However, the advantages of the above-described bispecific diabodies come at a salient cost. The formation of such non-mono-specific diabodies requires the successful assembly of two or more distinct and different polypeptides (i.e., such formation requires that the diabodies be formed through the heterodimerization of different polypeptide chain species). This fact is in contrast to mono-specific diabodies, which are formed through the homodimerization of identical polypeptide chains. Because at least two dissimilar polypeptides (i. e., two polypeptide species) must be provided in order to form a non-mono-specific diabody, and because homodimerization of such polypeptides leads to inactive molecules (Takemura, S. et al. (2000) “ Construction Of A Diabody  ( Small Recombinant Bispecific Antibody )  Using A Refolding System ,” Protein Eng. 13(8):583-588), the production of such polypeptides must be accomplished in such a way as to prevent covalent bonding between polypeptides of the same species (i. e., so as to prevent homodimerization) (Takemura, S. et al. (2000) “ Construction Of A Diabody  ( Small Recombinant Bispecific Antibody )  Using A Refolding System ,” Protein Eng. 13(8):583-588). The art has therefore taught the non-covalent association of such polypeptides (see, e.g., Olafsen et al. (2004) “ Covalent Disulfide - Linked Anti - CEA Diabody Allows Site - Specific Conjugation And Radiolabeling For Tumor Targeting Applications ,” Prot. Engr. Des. Sel. 17:21-27; Asano et al. (2004) “ A Diabody For Cancer Immunotherapy And Its Functional Enhancement By Fusion Of Human Fc Domain ,” Abstract 3P-683, J. Biochem. 76(8):992; Takemura, S. et al. (2000) “ Construction Of A Diabody  ( Small Recombinant Bispecific Antibody )  Using A Refolding System ,” Protein Eng. 13(8):583-588; Lu, D. et al. (2005) “ A Fully Human Recombinant IgG - Like Bispecific Antibody To Both The Epidermal Growth Factor Receptor And The Insulin - Like Growth Factor Receptor For Enhanced Antitumor Activity ,” J. Biol. Chem. 280(20):19665-19672). 
     However, the art has recognized that bispecific diabodies composed of non-covalently associated polypeptides are unstable and readily dissociate into non-functional single polypeptide chain monomers (see, e.g., Lu, D. et al. (2005) “ A Fully Human Recombinant IgG - Like Bispecific Antibody To Both The Epidermal Growth Factor Receptor And The Insulin - Like Growth Factor Receptor For Enhanced Antitumor Activity ,” J. Biol. Chem. 280(20):19665-19672). 
     In the face of this challenge, the art has succeeded in developing stable, covalently bonded heterodimeric non-mono-specific diabodies, termed DART® diabodies, see, e.g., Sloan, D. D. et al. (2015) “ Targeting HIV Reservoir in Infected CD 4  T Cells by Dual - Affinity Re - targeting Molecules  ( DARTs )  that Bind HIV Envelope and Recruit Cytotoxic T Cells ,” PLoS Pathog. 11(11):e1005233. doi: 10.1371/journal.ppat.1005233; Al Hussaini, M. et al. (2015) “ Targeting CD 123  In AML Using A T - Cell Directed Dual - Affinity Re - Targeting  ( DART ®)  Platform ,” Blood pii: blood-2014-05-575704; Chichili, G. R. et al. (2015) “ A CD 3 xCD 123  Bispecific DART For Redirecting Host T Cells To Myelogenous Leukemia: Preclinical Activity And Safety In Nonhuman Primates ,” Sci. Transl. Med. 7(289):289ra82; Johnson, S. et al. (2010) “ Effector Cell Recruitment With Novel Fv - Based Dual - Affinity Re - Targeting Protein Leads To Potent Tumor Cytolysis And In Vivo B - Cell Depletion ,” J. Molec. Biol. 399(3):436-449; Veri, M. C. et al. (2010) “ Therapeutic Control Of B Cell Activation Via Recruitment Of Fcgamma Receptor IIB  ( CD 32 B )  Inhibitory Function With A Novel Bispecific Antibody Scaffold ,” Arthritis Rheum. 62(7):1933-1943; Moore, P. A. et al. (2011) “ Application Of Dual Affinity Retargeting Molecules To Achieve Optimal Redirected T cell Killing Of B - Cell Lymphoma ,” Blood 117(17):4542-4551; U.S. Pat. Nos. 8,044,180; 8,133,982; 8,187,593; 8,193,318; 8,530,627; 8,669,349; 8,778,339; 8,784,808; 8,795,667; 8,802,091; 8,802,093; 8,946,387; 8,968,730; and 8,993,730; US Patent Publication Nos. 2009/0060910; 2010/0174053; 2011/0081347; 2011/0097323; 2011/0117089; 2012/0009186; 2012/0034221; 2012/0141476; 2012/0294796; 2013/0149236; 2013/0295121; 2014/0017237; and 2014/0099318; European Patent Documents No. EP 1868650; EP 2158221; EP 2247304; EP 2252631; EP 2282770; EP 2328934; EP 2376109; EP 2542256; EP 2601216; EP 2714079; EP 2714733; EP 2786762; EP 2839842; EP 2840091; and PCT Publication Nos. WO 2006/113665; WO 2008/157379; WO 2010/027797; WO 2010/033279; WO 2010/080538; WO 2011/109400; WO 2012/018687; WO 2012/162067; WO 2012/162068; WO 2014/159940; WO 2015/021089; WO 2015/026892; and WO 2015/026894). Such diabodies comprise two or more covalently complexed polypeptides and involve engineering one or more cysteine residues into each of the employed polypeptide species that permit disulfide bonds to form and thereby covalently bond one or more pairs of such polypeptide chains to one another. For example, the addition of a cysteine residue to the C-terminus of such constructs has been shown to allow disulfide bonding between the involved polypeptide chains, stabilizing the resulting diabody without interfering with the diabody&#39;s binding characteristics. 
     The simplest DART® diabody comprises two polypeptide chains each comprising three Domains ( FIGS. 1A-1B ). The first polypeptide chain comprises: (i) a Domain that comprises a binding region of a light chain variable Domain of the a first immunoglobulin (VL1), (ii) a second Domain that comprises a binding region of a heavy chain variable Domain of a second immunoglobulin (VH2), and (iii) a third Domain that serves to promote heterodimerization (a “Heterodimer-Promoting Domain”) with the second polypeptide chain and to covalently bond the first polypeptide to the second polypeptide chain of the diabody. The second polypeptide chain contains a complementary first Domain (a VL2 Domain), a complementary second Domain (a VH1 Domain) and a third Domain that complexes with the third Domain of the first polypeptide chain in order to promote heterodimerization (a “Heterodimer-Promoting Domain”) and covalent bonding with the first polypeptide chain. Such molecules are stable, potent and have the ability to simultaneously bind two or more antigens. In one embodiment, the third Domains of the first and second polypeptide chains each contain a cysteine (“©”) residue, which serves to bind the polypeptides together via a disulfide bond. The third Domain of one or both of the polypeptide chains may additionally possess the sequence of a CH2-CH3 Domain, such that complexing of the diabody polypeptides forms an Fc Domain that is capable of binding to the Fc receptor of cells (such as B lymphocytes, dendritic cells, natural killer cells, macrophages, neutrophils, eosinophils, basophils and mast cells). Many variations of such molecules have been described (see, e.g., United States Patent Publication Nos. 2013-0295121; 2010-0174053; 2007-0004909; 2009-0060910; European Patent Publication No. EP 2714079; EP 2601216; EP 2376109; EP 2158221 and PCT Publication Nos. WO 2012/162068; WO 2012/018687; WO 2010/080538; WO 2006/113665) and are provided herein. Many variations of such molecules have been described (see, e.g., United States Patent Publication Nos. 2015/0175697; 2014/0255407; 2014/0099318; 2013/0295121; 2010/0174053; 2009/0060910; 2007-0004909; European Patent Publication Nos. EP 2714079; EP 2601216; EP 2376109; EP 2158221; EP 1868650; and PCT Publication Nos. WO 2012/162068; WO 2012/018687; WO 2010/080538; WO 2006/113665), and are provided herein. 
     Alternative constructs are known in the art for applications where a bispecific or tetravalent molecule is desirable but an Fc is not required including, but not limited to, Bispecific T cell Engager molecules, also referred to as “BITE® antibodies” (see, e.g., PCT Publication Nos: WO 1993/11161; and WO 2004/106381) and tetravalent tandem antibodies, also referred to as “TandAbs®” (see, e.g. United States Patent Publication No: 2011-0206672; European Patent Publication No. EP 2371866, and; PCT Publication Nos. WO 1999/057150, WO 2003/025018, and WO 2013/013700). BiTEs are formed from a single polypeptide chain comprising tandem linked scFvs, while TandAbs are formed by the homo-dimerization of two identical polypeptide chains, each possessing a VH1, VL2, VH2, and VL2 Domain. 
     The present invention provides bispecific binding molecules that are capable of enhancing an immune response directed to the killing of a target cell (e.g., a cancer cell or a pathogen-infected cell, a pathogen, etc.) expressing a Disease Antigen. Such bispecific binding molecules are capable of binding a “First Epitope” and a “Second Epitope,” wherein one of such epitopes is an epitope of CD16 and the other of such epitopes is an epitope of a Disease Antigen (“DA”). It is irrelevant whether a particular epitope is designated as the first vs. the Second Epitope; such notation having relevance only with respect to the presence and orientation of the domains of the polypeptide chains of the binding molecules of the present invention. Thus, the bispecific molecules of the present invention comprise “VL CD16 ”/“VH CD16 ” Domains that are capable of binding an epitope of CD16, and “VL DA ”/“VH DA ” Domains that are capable of binding an epitope of a Disease Antigen. The instant invention particular encompasses bispecific diabodies, BiTEs, antibodies, and TandAbs produced using any of the methods provided herein. 
     A. Diabodies Lacking Fc Domains 
     In one embodiment, the CD16 Binding Molecules of the present invention will be bispecific diabodies and will comprise domains capable of binding both a first and a Second Epitope, but will lack an Fc Domain, and thus will be unable to bind FcγR molecules via an Fc-FcγR interaction. Such molecules are, however, able to bind to CD16 via the SDRs or CDRs of their CD16 Binding Domains. The absence of Fc domains thus serves to prevent the molecules from binding to non-CD16 FcγRs, such as the inhibitory receptor CD32B. 
     The first polypeptide chain of such an embodiment of bispecific diabodies preferably comprises, in the N-terminal to C-terminal direction: an N-terminus, the VL Domain of a monoclonal antibody capable of binding either the First or Second Epitope (i.e., either VL CD16  or VL DA ), a first intervening spacer peptide (Linker 1), a VH Domain of a monoclonal antibody capable of binding the epitope of the Disease Antigen (if such first polypeptide chain contains VL CD16 ) or a VH Domain of a monoclonal antibody capable of binding CD16 (if such first polypeptide chain contains VL DA ), a second intervening spacer peptide (Linker 2) optionally containing a cysteine residue, a Heterodimer-Promoting Domain and a C-terminus ( FIGS. 1A-1B ). 
     The second polypeptide chain of this embodiment of bispecific diabodies comprises, in the N-terminal to C-terminal direction: an N-terminus, the VL Domain of a monoclonal antibody capable of binding the First or Second Epitope (i.e., VL CD16  or VL DA , and being the VL Domain not selected for inclusion in the first polypeptide chain of the diabody), an intervening spacer peptide (Linker 1), a VH Domain of a monoclonal antibody capable of binding either the First or Second Epitope (i.e., VH CD16  or VH DA , and being the VH Domain not selected for inclusion in the first polypeptide chain of the diabody), a second intervening spacer peptide (Linker 2) optionally containing a cysteine residue, a Heterodimer-Promoting Domain and a C-terminus ( FIGS. 1A-1B ). The employed VL and VH Domains specific for a particular epitope are preferably obtained or derived from the same monoclonal antibody. However, such domains may be derived from different monoclonal antibodies provided that they associate to form a functional Binding Domain capable of immunospecifically binding such epitope. Such different antibodies are referred to herein as being “corresponding” antibodies. 
     The VL Domain of the first polypeptide chain interacts with the VH Domain of the second polypeptide chain to form a first functional Epitope Binding Domain that is specific for one of the epitopes (e.g., the First Epitope). Likewise, the VL Domain of the second polypeptide chain interacts with the VH Domain of the first polypeptide chain in order to form a second functional Epitope Binding Domain that is specific for the other epitope (i.e., the Second Epitope). Thus, the selection of the VL and VH Domains of the first and second polypeptide chains is “coordinated,” such that the two polypeptide chains of the diabody collectively comprise VL and VH Domains capable of binding both the First Epitope and the Second Epitope (i.e., they collectively comprise VL CD16 /VH CD16  and VL DA /VH DA ). 
     Most preferably, the length of the intervening spacer peptide (i.e., “Linker 1,” which separates such VL and VH Domains) is selected to substantially or completely prevent the VL and VH Domains of the polypeptide chain from binding one another (for example consisting of from 0, 1, 2, 3, 4, 5, 6, 7, 8 or 9 intervening linker amino acid residues). Thus, the VL and VH Domains of the first polypeptide chain are substantially or completely incapable of binding one another. Likewise, the VL and VH Domains of the second polypeptide chain are substantially or completely incapable of binding one another. A preferred intervening spacer peptide (Linker 1) has the sequence (SEQ ID NO:16): 
     
       
         
           
               
               
            
               
                   
                 GGGS GGGG. 
               
            
           
         
       
     
     The length and composition of the second intervening spacer peptide (“Linker 2”) is selected based on the choice of one or more polypeptide domains that promote such dimerization (i.e., a “Heterodimer-Promoting Domain”). Typically, the second intervening spacer peptide (Linker 2) will comprise 3-20 amino acid residues. In particular, where the employed Heterodimer-Promoting Domain(s) do/does not comprise a cysteine residue a cysteine-containing second intervening spacer peptide (Linker 2) is utilized. A cysteine-containing second intervening spacer peptide (Linker 2) will contain 1, 2, 3 or more cysteines. A preferred cysteine-containing spacer peptide (Linker 2) has the sequence GGCGGG (SEQ ID NO:17). Alternatively, Linker 2 does not comprise a cysteine (e.g., GGG, GGGS (SEQ ID NO:18), LGGGSG (SEQ ID NO:19), GGGSGGGSGGG (SEQ ID NO:20), AS TKG (SEQ ID NO:21), LEPKSS (SEQ ID NO:22), APSSS (SEQ ID NO:23), etc.) and a cysteine-containing Heterodimer-Promoting Domain, as described below is used. Optionally, both a cysteine-containing Linker 2 and a cysteine-containing Heterodimer-Promoting Domain are used. 
     The Heterodimer-Promoting Domains may be GVEPKSC (SEQ ID NO:24) or VEPKSC (SEQ ID NO:25) or AEPKSC (SEQ ID NO:26) on one polypeptide chain and GFNRGEC (SEQ ID NO:27) or FNRGEC (SEQ ID NO:28) on the other polypeptide chain (US2007/0004909). 
     In a preferred embodiment, the Heterodimer-Promoting Domains will comprise tandemly repeated coil domains of opposing charge for example, an “E-coil” Heterodimer-Promoting Domain (SEQ ID NO:29:  E VAAL E K- E VAAL E K- E VAAL E K- E VAAL E K), whose glutamate residues will form a negative charge at pH 7, or a “K-coil” Heterodimer-Promoting Domain (SEQ ID NO:30:  K VAAL K E- K VAAL K E- K VAAL K E  K VAAL K E), whose lysine residues will form a positive charge at pH 7. The presence of such charged domains promotes association between the first and second polypeptides, and thus fosters heterodimer formation. Heterodimer-Promoting Domains that comprise modifications of the above-described E-coil and K-coil sequences so as to include one or more cysteine residues may be utilized. The presence of such cysteine residues permits the coil present on one polypeptide chain to become covalently bonded to a complementary coil present on another polypeptide chain, thereby covalently bonding the polypeptide chains to one another and increasing the stability of the diabody. Examples of such particularly preferred are Heterodimer-Promoting Domains include a Modified E-Coil having the amino acid sequence  E VAA CE K- E VAAL E K- E VAAL E K- E VAAL E K (SEQ ID NO:31), and a modified K-coil having the amino acid sequence  K VAA CK E- K VAAL K E- K VAAL K E- K VAAL K E (SEQ ID NO:32). 
     As disclosed in WO 2012/018687, in order to improve the in vivo pharmacokinetic properties of diabodies, a diabody may be modified to contain a polypeptide portion of a serum-binding protein at one or more of the termini of the diabody. Most preferably, such polypeptide portion of a serum-binding protein will be installed at the C-terminus of a polypeptide chain of the diabody. Albumin is the most abundant protein in plasma and has a half-life of 19 days in humans. Albumin possesses several small molecule binding domains that permit it to non-covalently bind other proteins and thereby extend their serum half-lives. The Albumin-Binding Domain 3 (ABD3) of protein G of  Streptococcus  strain G148 consists of 46 amino acid residues forming a stable three-helix bundle and has broad albumin-binding specificity (Johansson, M. U. et al. (2002) “ Structure, Specificity, And Mode Of Interaction For Bacterial Albumin - Binding Modules ,” J. Biol. Chem. 277(10):8114-8120). Thus, a particularly preferred polypeptide portion of a serum-binding protein for improving the in vivo pharmacokinetic properties of a diabody is the Albumin-Binding Domain (ABD) from streptococcal protein G, and more preferably, the Albumin-Binding Domain 3 (ABD3) of protein G of  Streptococcus  strain G148 (SEQ ID NO:33): 
     
       
         
           
               
               
            
               
                   
                 LAEAKVLANR ELDKYGVSDY YKNLIDNAKS 
               
               
                   
               
               
                   
                 AEGVKALIDE ILAALP. 
               
            
           
         
       
     
     As disclosed in WO 2012/162068 (herein incorporated by reference), “deimmunized” variants of SEQ ID NO:33 have the ability to attenuate or eliminate MHC class II binding. Based on combinational mutation results, the following combinations of substitutions are considered to be preferred substitutions for forming such a deimmunized ABD: 66D/70S+71A; 66S/70S+71A; 66S/70S+79A; 64A/65A/71A; 64A/65A/71A+66S; 64A/65A/71A+66D; 64A/65A/71A+66E; 64A/65A/79A+66S; 64A/65A/79A+66D; 64A/65A/79A+66E. Variant ABDs having the modifications L64A, I65A and D79A or the modifications N66S, T7OS and D79A. Variant deimmunized ABD having the amino acid sequence: 
                            (SEQ ID NO: 34)           LAEAKVLANR ELDKYGVSDY YKNLI   D     66 NAK   S     70                         A     71 EGVKALIDE ILAALP,            
or the amino acid sequence:
 
                            (SEQ ID NO: 35)           LAEAKVLANR ELDKYGVSDY YKN   A     64     A     65 NNAKT                   VEGVKALI   A     79 E ILAALP,            
or the amino acid sequence:
 
                            (SEQ ID NO: 36)           LAEAKVLANR ELDKYGVSDY YKNLI   S     66 NAK   S     70                     VEGVKALI   A     79 E ILAALP,            
are particularly preferred as such deimmunized ABD exhibit substantially wild-type binding while providing attenuated MHC class II binding. Thus, the first polypeptide chain of such a diabody having an ABD contains a third linker (Linker 3) preferably positioned C-terminally to the E-coil (or K-coil) Domain of such polypeptide chain so as to intervene between the E-coil (or K-coil) Domain and the ABD (which is preferably a deimmunized ABD). A preferred sequence for such Linker 3 is SEQ ID NO:18:
 
     
       
         
           
               
               
            
               
                   
                 GGGS. 
               
            
           
         
       
     
     B. Diabodies Comprising Fc Domains 
     One embodiment of the present invention relates to multi-specific diabodies (e.g., bispecific, trispecific, tetraspecific, etc.) that comprise an Fc Domain and that are capable of simultaneously binding an epitope of CD16 and an epitope of a Disease Antigen. The Fc Domain of such molecules may be of any isotype (e.g., IgG1, IgG2, IgG3, or IgG4). The molecules may further comprise a CH1 Domain and/or a Hinge Domain. When present, the CH1 Domain and/or Hinge Domain may be of any isotype (e.g., IgG1, IgG2, IgG3, or IgG4), and is preferably of the same isotype as the desired Fc Domain. 
     The addition of an IgG CH2-CH3 Domain to one or both of the diabody polypeptide chains, such that the complexing of the diabody chains results in the formation of an Fc Domain, increases the biological half-life and/or alters the valency of the diabody. Such diabodies comprise, two or more polypeptide chains whose sequences permit the polypeptide chains to covalently bind each other to form a covalently associated diabody that is capable of simultaneously binding the First Epitope and the Second Epitope. Incorporating an IgG CH2-CH3 Domains onto both of the diabody polypeptides will permit a two-chain bispecific Fc Domain-containing diabody to form ( FIG. 2 ). 
     Alternatively, incorporating IgG CH2-CH3 Domains onto only one of the diabody polypeptides will permit a more complex four-chain bispecific Fc Domain-containing diabody to form ( FIGS. 3A-3C ).  FIG. 3C  shows a representative four-chain diabody possessing the Constant Light (CL) Domain and the Constant Heavy CH1 Domain, however fragments of such domains as well as other polypeptides may alternatively be employed (see, e.g.,  FIGS. 3A and 3B , United States Patent Publication Nos. 2013-0295121; 2010-0174053 and 2009-0060910; European Patent Publication No. EP 2714079; EP 2601216; EP 2376109; EP 2158221 and PCT Publication Nos. WO 2012/162068; WO 2012/018687; WO 2010/080538). Thus, for example, in lieu of the CH1 Domain, one may employ a peptide having the amino acid sequence GVEPKSC (SEQ ID NO:24), VEPKSC (SEQ ID NO:25), or AEPKSC (SEQ ID NO:26), derived from the Hinge Domain of a human IgG, and in lieu of the CL Domain, one may employ the C-terminal 6 amino acids of the human kappa Light Chain, GFNRGEC (SEQ ID NO:27) or FNRGEC (SEQ ID NO:28). A representative peptide containing four-chain diabody is shown in  FIG. 3A . Alternatively, or in addition, one may employ a peptide comprising tandem coil domains of opposing charge such as the “E-coil” helical domains 
                            (SEQ ID NO: 29:                E   VAAL   E   K-   E   VAAL   E   K-   E   VAAL   E   K-   E   VAAL   E   K           or                   SEQ ID NO: 31:                E   VAA   CE   K-   E   VAAL   E   K-   E   VAAL   E   K-   E   VAAL   E   K);           and                   the “K-coil” domains           (SEQ ID NO: 30:                K   VAAL   K   E-   K   VAAL   K   E-   K   VAAL   K   E-   K   VAAL   K   E           or                   SEQ ID NO: 32:                K   VAA   CK   E-   K   VAAL   K   E-   K   VAAL   K   E-   K   VAAL   K   E).            
A representative coil domain containing four-chain diabody is shown in  FIG. 3B .
 
     Fc Domain-containing diabody molecules of the present invention may include additional intervening spacer peptides (Linkers), generally such Linkers will be incorporated between a Heterodimer-Promoting Domain (e.g., an E-coil or K-coil) and a CH2-CH3 Domain and/or between a CH2-CH3 Domain and a Variable Domain (i.e., VH or VL). Typically, the additional Linkers will comprise 3-20 amino acid residues and may optionally contain all or a portion of an IgG Hinge Domain (preferably a cysteine-containing portion of an IgG Hinge Domain possessing 1, 2, 3 or more cysteine residues). Linkers that may be employed in the bispecific Fc Domain-containing diabody molecules of the present invention include: GGGS (SEQ ID NO:18), LGGGSG (SEQ ID NO:19), GGGSGGGSGGG (SEQ ID NO:20), AS TKG (SEQ ID NO:21), LEPKSS (SEQ ID NO:22), APSSS (SEQ ID NO:23), APSSSPME (SEQ ID NO:37), VEPKSADKTHTCPPCP (SEQ ID NO:38), LEPKSADKTHTCPPCP (SEQ ID NO:39), DKTHTCPPCP (SEQ ID NO:40), the scFv linker: GGGGSGGGGSGGGGS (SEQ ID NO:41); the “long” linker: GGGGSGGGSGGG (SEQ ID NO:42), GGC, and GGG. LEPKSS (SEQ ID NO:22) may be used in lieu of GGG or GGC for ease of cloning. Additionally, the amino acids GGG, or LEPKSS (SEQ ID NO:22) may be immediately followed by DKTHTCPPCP (SEQ ID NO:40) to form the alternate linkers: GGGDKTHTCPPCP (SEQ ID NO:43); and LEPKSSDKTHTCPPCP (SEQ ID NO:44). 
     Bispecific Fc Domain-containing molecules of the present invention may incorporate an IgG Hinge Domain in addition to or in place of a linker. Exemplary Hinge Domains include: EPKSCDKTHTCPPCP (SEQ ID NO:5) from IgG1, ERKCCVECPPCP (SEQ ID NO:6) from IgG2, ELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPEPKS CDTPPPCPRCP (SEQ ID NO:7) from IgG3, ESKYGPPCPSCP (SEQ ID NO:8) from IgG4, and ESKYGPPCP P CP (SEQ ID NO:9) an IgG4 Hinge variant comprising a stabilizing S228P substitution (as numbered by the EU index as set forth in Kabat) to reduce strand exchange. 
     As provided in  FIG. 3A-3C , Fc Domain-containing diabodies of the invention may comprise four chains. The first and third polypeptide chains of such a diabody contain three domains: (i) a VL1-containing Domain, (ii) a VH2-containing Domain, (iii) a Heterodimer-Promoting Domain, and (iv) a Domain containing a CH2-CH3 sequence. The second and fourth polypeptide chains contain: (i) a VL2-containing Domain, (ii) a VH1-containing Domain, and (iii) a Heterodimer-Promoting Domain, where the Heterodimer-Promoting Domains promote the dimerization of the first/third polypeptide chains with the second/fourth polypeptide chains. The VL and/or VH Domains of the third and fourth polypeptide chains, and VL and/or VH Domains of the first and second polypeptide chains may be the same or different so as to permit tetravalent binding that is either mono-specific, bispecific or tetraspecific. The notation “VL3” and “VH3” denote respectively, the Light Chain Variable Domain and Variable Heavy Chain Domain that bind a “third” epitope of such diabody. Similarly, the notation “VL4” and “VH4” denote respectively, the Light Chain Variable Domain and Variable Heavy Chain Domain that bind a “fourth” epitope of such diabody. The general structure of the polypeptide chains of a representative four-chain bispecific Fc Domain-containing diabodies of invention is provided in Table 1: 
     
       
         
           
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
             
            
               
                 Bispecific 
                 2 nd  Chain 
                 NH 2 -VL2-VH1-HPD-COOH 
               
               
                   
                 1 s  Chain 
                 NH 2 -VL1-VH2-HPD-CH2-CH3-COOH 
               
               
                   
                 1 st  Chain  
                 NH 2 -VL1-VH2-HPD-CH2-CH3-COOH 
               
               
                   
                 2 nd  Chain 
                 NH 2 -VL2-VH1-HPD-COOH 
               
               
                 Tetraspecific 
                 2 nd  Chain 
                 NH 2 -VL2-VH1-HPD-COOH 
               
               
                   
                 1 st  Chain  
                 NH 2 -VL1-VH2-HPD-CH2-CH3-COOH 
               
               
                   
                 3 rd  Chain  
                 NH 2 -VL3-VH4-HPD-CH2-CH3-COOH 
               
               
                   
                 4 th  Chain 
                 NH 2 -VL4-VH3-HPD-COOH 
               
               
                   
               
               
                 HPD = Heterodimer-Promoting Domain 
               
            
           
         
       
     
     In a specific embodiment, diabodies of the present invention are bispecific, tetravalent (i.e., possess four Epitope Binding Domains), Fc-containing diabodies that are composed of four total polypeptide chains ( FIGS. 3A-3C ). The bispecific, tetravalent, Fc-containing diabodies of the invention comprise two First Epitope Binding Domains and two Second Epitope Binding Domains. 
     In a further embodiment, the Fc Domain-containing diabodies of the present invention may comprise three polypeptide chains. The first polypeptide of such a diabody contains three domains: (i) a VL1-containing Domain, (ii) a VH2-containing Domain and (iii) a Domain containing a CH2-CH3 sequence. The second polypeptide of such a diabody contains: (i) a VL2-containing Domain, (ii) a VH1-containing Domain and (iii) a Domain that promotes heterodimerization and covalent bonding with the diabody&#39;s first polypeptide chain. The third polypeptide of such a diabody comprises a CH2-CH3 sequence. Thus, the first and second polypeptide chains of such a diabody associate together to form a VL1/VH1 Epitope Binding Domain that is capable of binding either the First or Second Epitope, as well as a VL2/VH2 Epitope Binding Domain that is capable of binding the other of such epitopes. The first and second polypeptides are bonded to one another through a disulfide bond involving cysteine residues in their respective Third Domains. Notably, the first and third polypeptide chains complex with one another to form an Fc Domain that is stabilized via a disulfide bond. Such bispecific diabodies have enhanced potency.  FIGS. 4A and 4B  illustrate the structures of such diabodies. Such Fc Domain-containing diabodies may have either of two orientations (Table 2): 
     
       
         
           
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
             
            
               
                 First 
                 3 rd  Chain  
                 NH 2 -CH2-CH3-COOH 
               
               
                 Orientation 
                 1 st  Chain  
                 NH 2 -VL1-VH2-HPD-CH2-CH3-COOH 
               
               
                   
                 2 nd  Chain 
                 NH 2 -VL2-VH1-HPD-COOH 
               
               
                 Second 
                 3 rd  Chain  
                 NH 2 -CH2-CH3-COOH 
               
               
                 Orientation 
                 1 st  Chain 
                 NH 2 -CH2-CH3-VL1-VH2-HPD-COOH 
               
               
                   
                 2 nd  Chain 
                 NH 2 -VL2-VH1-HPD-COOH 
               
               
                   
               
               
                 HPD = Heterodimer-Promoting Domain 
               
            
           
         
       
     
     In a specific embodiment, diabodies of the present invention are bispecific, bivalent (i.e., possess two Epitope Binding Domains), Fc-containing diabodies that are composed of three total polypeptide chains ( FIGS. 4A-4B ). The bispecific, bivalent Fc-containing diabodies of the invention comprise one Epitope Binding Domain immunospecific for either the First or Second Epitope, as well as a VL2/VH2 Epitope Binding Domain that is capable of binding the other of such epitopes. 
     In a further embodiment, the Fc Domain-containing diabodies may comprise a total of five polypeptide chains. In a particular embodiment, two of the five polypeptide chains have the same amino acid sequence. The first polypeptide chain of such a diabody contains: (i) a VH1-containing Domain, (ii) a CH1-containing Domain, and (iii) a Domain containing a CH2-CH3 sequence. The first polypeptide chain may be the Heavy Chain of an antibody that contains a VH1 and a Heavy Chain constant region. The second and fifth polypeptide chains of such a diabody contain: (i) a VL1-containing Domain, and (ii) a CL-containing Domain. The second and/or fifth polypeptide chains of such a diabody may be Light Chains of an antibody that contains a VL1 complementary to the VH1 of the first/third polypeptide chain. The first, second and/or fifth polypeptide chains may be isolated from a naturally occurring antibody. Alternatively, they may be constructed recombinantly. The third polypeptide chain of such a diabody contains: (i) a VH1-containing Domain, (ii) a CH1-containing Domain, (iii) a Domain containing a CH2-CH3 sequence, (iv) a VL2-containing Domain, (v) a VH3-containing Domain and (vi) a Heterodimer-Promoting Domain, where the Heterodimer-Promoting Domains promote the dimerization of the third chain with the fourth chain. The fourth polypeptide of such diabodies contains: (i) a VL3-containing Domain, (ii) a VH2-containing Domain and (iii) a Domain that promotes heterodimerization and covalent bonding with the diabody&#39;s third polypeptide chain. 
     Thus, the first and second, and the third and fifth, polypeptide chains of such diabodies associate together to form two VL1/VH1 Epitope Binding Domains capable of binding a First Epitope. The third and fourth polypeptide chains of such diabodies associate together to form a VL2/VH2 Epitope Binding Domain that is capable of binding a Second Epitope, as well as a VL3/VH3 Epitope Binding Domain that is capable of binding a Third Epitope. The first and third polypeptides are bonded to one another through a disulfide bond involving cysteine residues in their respective constant regions. Notably, the first and third polypeptide chains complex with one another to form an Fc Domain. Such multispecific diabodies have enhanced potency.  FIG. 5  illustrates the structure of such diabodies. It will be understood that the VL1/VH1, VL2/VH2, and VL3/VH3 Domains may be the same or different so as to permit binding that is mono-specific, bispecific or trispecific. 
     The VL and VH Domains of the polypeptide chains are selected so as to form VL/VH Epitope Binding Domains specific for a desired epitope. The VL/VH Epitope Binding Domains formed by the association of the polypeptide chains may be the same or different so as to permit tetravalent binding that is mono-specific, bispecific, trispecific or tetraspecific. In particular, the VL and VH Domains maybe selected such that a multivalent diabody may comprise two Binding Domains for a First Epitope and two Binding Domains for a Second Epitope, or three Binding Domains for a First Epitope and one Binding Domain for a Second Epitope, or two Binding Domains for a First Epitope, one Binding Domain for a Second Epitope and one Binding Domain for a Third Epitope (as depicted in  FIG. 5 ). The general structure of the polypeptide chains of representative five-chain Fc Domain-containing diabodies of invention is provided in Table 3: 
     
       
         
           
               
               
               
             
               
                 TABLE 3 
               
               
                   
               
             
            
               
                 Bispecific  
                 2 nd  Chain 
                 NH 2 -VL1-CL-COOH 
               
               
                 (2 × 2) 
                 1 st  Chain  
                 NH 2 -VH1-CH1-CH2-CH3-COOH 
               
               
                   
                 3 rd  Chain  
                 NH 2 -VH1-CH1-CH2-CH3- 
               
               
                   
                   
                 VL2-VH2-HPD-COOH 
               
               
                   
                 5 nd  Chain 
                 NH 2 -VL1-CL-COOH 
               
               
                   
                 4t th  Chain 
                 NH 2 -VL2-VH2-HPD-COOH 
               
               
                 Bispecific  
                 2 nd  Chain 
                 NH 2 -VL1-CL-COOH 
               
               
                 (3 × 1) 
                 1 st  Chain 
                 NH 2 -VH1-CH1-CH2-CH3-COOH 
               
               
                   
                 3 rd  Chain  
                 NH 2 -VH1-CH1-CH2-CH3- 
               
               
                   
                   
                 VL1-VH2-HPD-COOH 
               
               
                   
                 5 nd  Chain 
                 NH 2 -VL1-CL-COOH 
               
               
                   
                 4 th  Chain 
                 NH 2 -VL2-VH1-HPD-COOH 
               
               
                 Trispecific  
                 2 nd  Chain 
                 NH 2 -VL1-CL-COOH 
               
               
                 (2 × 1 × 1) 
                 1 st  Chain 
                 NH 2 -VH1-CH1-CH2-CH3-COOH 
               
               
                   
                 3 rd  Chain  
                 NH 2 -VH1-CH1-CH2-CH3- 
               
               
                   
                   
                 VL2-VH3-HPD-COOH 
               
               
                   
                 5 nd  Chain 
                 NH 2 -VL1-CL-COOH 
               
               
                   
                 4 th  Chain 
                 NH 2 -VL3-VH2-HPD-COOH 
               
               
                   
               
               
                 HPD = Heterodimer-Promoting Domain 
               
            
           
         
       
     
     In a specific embodiment, diabodies of the present invention are bispecific, tetravalent (i.e., possess four Epitope Binding Domains), Fc-containing diabodies that are composed of five total polypeptide chains having two Epitope Binding Domains immunospecific for the First Epitope, and two Epitope Binding Domains specific for the Second Epitope. In another embodiment, the bispecific, tetravalent, Fc-containing diabodies of the invention comprise three Epitope Binding Domains immunospecific for the First Epitope and one Epitope Binding Domain specific for the Second Epitope. As provided above, the VL and VH Domains may be selected to permit trispecific binding. Accordingly, the invention also encompasses trispecific, tetravalent, Fc-containing diabodies. The trispecific, tetravalent, Fc-containing diabodies of the invention comprise two Epitope Binding Domains immunospecific for the First Epitope, one Epitope Binding Domain immunospecific for the Second Epitope, and one Epitope Binding Domain immunospecific for the Third Epitope. 
     In traditional immune function, the interaction of antibody-antigen complexes with cells of the immune system results in a wide array of responses, ranging from effector functions such as antibody-dependent cytotoxicity, mast cell degranulation, and phagocytosis to immunomodulatory signals such as regulating lymphocyte proliferation and antibody secretion. All of these interactions are initiated through the binding of the Fc Domain of antibodies or immune complexes to specialized cell surface receptors on hematopoietic cells. As discussed above, the diversity of cellular responses triggered by antibodies and immune complexes results from the structural heterogeneity of the three Fc receptors: FcγRT (CD64), FcγRII (CD32), and FcγRIII (CD16). FcγRT (CD64), FcγRIIA (CD32A) and FcγRIII (CD16) are activating (i.e., immune system enhancing) receptors; FcγRIIB (CD32B) is an inhibiting (i.e., immune system dampening) receptor. In addition, interaction with the neonatal Fc Receptor (FcRn) mediates the recycling of IgG molecules from the endosome to the cell surface and release into the blood. The amino acid sequence of exemplary wild-type IgG1 (SEQ ID NO:12), IgG2 (SEQ ID NO:13), IgG3 (SEQ ID NO:14), and IgG4 (SEQ ID NO:15) are presented above. 
     Modification of the Fc Domain may lead to an altered phenotype, for example altered serum half-life, altered stability, altered susceptibility to cellular enzymes or altered effector function. It may therefore be desirable to modify an Fc Domain-containing binding molecule of the present invention with respect to effector function, for example, so as to enhance the effectiveness of such molecule in treating cancer. Reduction or elimination of Fc Domain-mediated effector function is desirable in certain cases, for example in the case of antibodies whose mechanism of action involves blocking or antagonism, but not killing of the cells bearing a target antigen. Increased effector function is generally desirable when directed to undesirable cells, such as tumor and foreign cells, where the FcγRs are expressed at low levels, for example, tumor-specific B cells with low levels of FcγRIIB (e.g., non-Hodgkin&#39;s lymphoma, CLL, and Burkitt&#39;s lymphoma). Molecules of the invention possessing such conferred or altered effector function activity are useful for the treatment and/or prevention of a disease, disorder or infection in which an enhanced efficacy of effector function activity is desired. 
     Accordingly, in certain embodiments, the Fc Domain of the Fc Domain-containing molecules of the present invention may be an engineered variant Fc Domain. Although the Fc Domain of the bispecific Fc Domain-containing molecules of the present invention may possess the ability to bind one or more Fc receptors (e.g., FcγR(s)), more preferably such variant Fc Domain have altered binding FcγRIA (CD64), FcγRIIA (CD32A), FcγRIIB (CD32B), FcγRIIIA (CD16a) or FcγRIIIB (CD16b) (relative to the binding exhibited by a wild-type Fc Domain), e.g., will have enhanced binding an activating receptor and/or will have substantially reduced or no ability to bind inhibitory receptor(s). Thus, the Fc Domain of the Fc Domain-containing molecules of the present invention may include some or all of the CH2 Domain and/or some or all of the CH3 Domain of a complete Fc Domain, or may comprise a variant CH2 and/or a variant CH3 sequence (that may include, for example, one or more insertions and/or one or more deletions with respect to the CH2 or CH3 domains of a complete Fc Domain). Such Fc Domains may comprise non-Fc polypeptide portions, or may comprise portions of non-naturally complete Fc Domains, or may comprise non-naturally occurring orientations of CH2 and/or CH3 Domains (such as, for example, two CH2 Domains or two CH3 Domains, or in the N-terminal to C-terminal direction, a CH3 Domain linked to a CH2 Domain, etc.). 
     Fc Domain modifications identified as altering effector function are known in the art, including modifications that increase binding activating receptors (e.g., FcγRIIA (CD16A) and reduce binding inhibitory receptors (e.g., FcγRIIB (CD32B) (see, e.g., Stavenhagen, J. B. et al. (2007) “ Fc Optimization Of Therapeutic Antibodies Enhances Their Ability To Kill Tumor Cells In Vitro And Controls Tumor Expansion In Vivo Via Low - Affinity Activating Fcgamma Receptors ,” Cancer Res. 57(18):8882-8890). Table 4 lists exemplary single, double, triple, quadruple and quintuple substitutions (numbering (according to the EU index) and substitutions are relative to the amino acid sequence of SEQ ID NO:12 as presented above) of exemplary modification that increase binding activating receptors and/or reduce binding inhibitory receptors. 
     
       
         
           
               
             
               
                 TABLE 4 
               
               
                   
               
               
                 Variations of Preferred Activating Fc Domains† 
               
               
                   
               
             
            
               
                 Single-Site Variations 
               
            
           
           
               
               
               
               
            
               
                 F243L 
                 R292G 
                 D270E 
                 R292P 
               
               
                 Y300L 
                 P396L 
                   
                   
               
            
           
           
               
            
               
                 Double-SiteVariations 
               
            
           
           
               
               
               
               
            
               
                 F243L and  
                 F243L and  
                 F243L and  
                 R292P and  
               
               
                 R292P 
                 Y300L 
                 P396L 
                 Y300L 
               
               
                 D270E and  
                 R292P and  
                 P396L and  
                 P247L and 
               
               
                 P396L 
                 V305I 
                 Q419H 
                 N421K 
               
               
                 R292P and  
                 Y300L and  
                 R255L and  
                 R292P and 
               
               
                 P396L 
                 P396L 
                 P396L 
                 P305I 
               
               
                 K392T and  
                   
                   
                   
               
               
                 P396L 
                   
                   
                   
               
            
           
           
               
            
               
                 Triple-Site Variations 
               
            
           
           
               
               
            
               
                 F243L, P247L and N421K 
                 P247L, D270E and N421K 
               
               
                 F243L, R292P and Y300L 
                 R255L, D270E and P396L 
               
               
                 F243L, R292P and V305I 
                 D270E, G316D and R416G 
               
               
                 F243L, R292P and P396L 
                 D270E, K392T and P396L 
               
               
                 F243L, Y300L and P396L 
                 D270E, P396L and Q419H 
               
               
                 V284M, R292L and K370N 
                 R292P, Y300L and P396L 
               
            
           
           
               
            
               
                 Quadruple-Site Variations 
               
            
           
           
               
               
            
               
                 L234F, F243L, R292P and Y300L 
                 F243L, P247L, D270E and N421K 
               
               
                 L234F, F243L, R292P and Y300L 
                 F243L, R255L, D270E and P396L 
               
               
                 L235I, F243L, R292P and Y300L 
                 F243L, D270E, G316D and R416G 
               
               
                 L235Q, F243L, R292P and Y300L 
                 F243L, D270E, K392T and P396L 
               
               
                 P247L, D270E, Y300L and N421K 
                 F243L, R292P, Y300L, and P396L 
               
               
                 R255L, D270E, R292G and P396L 
                 F243L, R292P, V305I and P396L 
               
               
                 R255L, D270E, Y300L and P396L 
                 F243L, D270E, P396L and Q419H 
               
               
                 D270E, G316D, P396L and R416G 
                   
               
            
           
           
               
            
               
                 Quintuple-SiteVariations 
               
            
           
           
               
               
            
               
                 L235V, F243L, R292P,  
                 F243L, R292P, V305I,  
               
               
                 Y300L and P396L 
                 Y300L and P396L 
               
               
                 L235P, F243L, R292P,  
                   
               
               
                 Y300L and P396L 
               
               
                   
               
               
                 †numbering is according to the EU index as in Kabat 
               
            
           
         
       
     
     Exemplary variants of human IgG1 Fc Domains with reduced binding CD32B and/or increased binding CD16A contain F243L, R292P, Y300L, V305I or P396L substitutions, wherein the numbering is that of the EU index as in Kabat. These amino acid substitutions may be present in a human IgG1 Fc Domain in any combination. In one embodiment, the variant human IgG1 Fc Domain contains a F243L, R292P and Y300L substitution. In another embodiment, the variant human IgG1 Fc Domain contains a F243L, R292P, Y300L, V305I and P396L substitution. 
     In certain embodiments, it is preferred for the Fc Domains of the Fc Domain-containing binding molecules of the present invention to exhibit decreased (or substantially no) binding FcγRIA (CD64), FcγRIIA (CD32A), FcγRIIB (CD32B), FcγRIIIA (CD16a) or FcγRIIIB (CD16b) (relative to the binding exhibited by the wild-type IgG1 Fc Domain (SEQ ID NO:12). In a specific embodiment, the Fc Domain-containing binding molecules of the present invention comprise an IgG Fc Domain that exhibits reduced antibody-dependent cell-mediated cytotoxicity (ADCC) effector function. In a preferred embodiment, the CH2-CH3 Domains of such binding molecules include any 1, 2, 3, or 4 of the substitutions: L234A, L235A, D265A, N297Q, and N297G, wherein the numbering is that of the EU index as in Kabat. In another embodiment, the CH2-CH3 Domains contain an N297Q substitution, an N297G substitution, L234A and L235A substitutions or a D265A substitution, as these mutations abolish FcR binding. Alternatively, a CH2-CH3 Domain of a naturally occurring Fc Domain that inherently exhibits decreased (or substantially no) binding FcγRIIIA (CD16a) and/or reduced effector function (relative to the binding and effector function exhibited by the wild-type IgG1 Fc Domain (SEQ ID NO:12)) is utilized. In a specific embodiment, the Fc Domain-containing binding molecules of the present invention comprise an IgG2 Fc Domain (SEQ ID NO:13), an IgG3 Fc Domain (SEQ ID NO:14) or an IgG4 Fc Domain (SEQ ID NO:15). When an IgG4 Fc Domain is utilized, the instant invention also encompasses the introduction of a stabilizing mutation, such as the Hinge Region S228P substitution described above (see, e.g., SEQ ID NO:11). Since the N297G, N297Q, L234A, L235A and D265A substitutions abolish effector function, in circumstances in which effector function is desired, these substitutions would preferably not be employed. 
     A preferred IgG1 sequence for the CH2 and CH3 Domains of the Fc Domain-containing molecules of the present invention having reduced or abolished effector function will comprise the substitutions L234A/L235A (SEQ ID NO:45): 
     
       
         
           
               
               
            
               
                   
                 APE   AA   GGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED 
               
               
                   
               
               
                   
                 PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 
               
               
                   
               
               
                   
                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 
               
               
                   
               
               
                   
                 LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN 
               
               
                   
               
               
                   
                 YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE 
               
               
                   
               
               
                   
                 ALHNHYTQKS LSLSPG   X     
               
            
           
         
       
     
     wherein  X  is lysine (K) or is absent. 
     A second preferred IgG1 sequence for the CH2 and CH3 Domains of the Fc Region-containing molecules of the present invention comprises an S442C substitution (shown underlined), so as to permit two CH3 domains to be covalently bonded to one another via a disulfide bond or to permit conjugation of a drug moiety. The amino acid sequence of such molecule is (SEQ ID NO:46): 
                            APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED                   PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH                   QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT                   LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN                   YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE                   ALHNHYTQKS L   C   LSPG   X                
wherein  X  is lysine (K) or is absent.
 
     A third preferred IgG1 sequence for the CH2 and CH3 Domains of the Fc Region-containing molecules of the present invention comprises the L234A/L235A substitutions (shown underlined) that reduce or abolish effector function and the S442C substitution (shown underlined) that permits two CH3 domains to be covalently bonded to one another via a disulfide bond or conjugation of a drug moiety. The amino acid sequence of such molecule is (SEQ ID NO:47): 
                            APE   AA   GGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED                   PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH                   QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT                   LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN                   YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE                   ALHNHYTQKS L   C   LSPG   X                
wherein  X  is lysine (K) or is absent.
 
     The serum half-life of proteins comprising Fc Domains may be increased by increasing the binding affinity of the Fc Domain for FcRn. The term “half-life” as used herein means a pharmacokinetic property of a molecule that is a measure of the mean survival time of the molecules following their administration. Half-life can be expressed as the time required to eliminate fifty percent (50%) of a known quantity of the molecule from a subject&#39;s body (e.g., a human patient or other mammal) or a specific compartment thereof, for example, as measured in serum, i.e., circulating half-life, or in other tissues. In general, an increase in half-life results in an increase in mean residence time (MRT) in circulation for the molecule administered. 
     In some embodiments, the Fc Domain-containing binding molecules of the present invention comprise a variant Fc Domain that comprises at least one amino acid modification relative to a wild-type Fc Domain, such that the molecule has an increased half-life (relative to such molecule if comprising a wild-type Fc Domain). In some embodiments, the Fc Domain-containing binding molecules of the present invention comprise a variant IgG Fc Domain that comprises a half-life extending amino acid substitution at one or more positions selected from the group consisting of 238, 250, 252, 254, 256, 257, 256, 265, 272, 286, 288, 303, 305, 307, 308, 309, 311, 312, 317, 340, 356, 360, 362, 376, 378, 380, 382, 413, 424, 428, 433, 434, 435, and 436, wherein the numbering is that of the EU index as in Kabat. Numerous mutations capable of increasing the half-life of an Fc Domain-containing molecule are known in the art and include, for example M252Y, S254T, T256E, and combinations thereof. For example, see the mutations described in U.S. Pat. Nos. 6,277,375, 7,083,784; 7,217,797, 8,088,376; U.S. Publication Nos. 2002/0147311; 2007/0148164; and PCT Publication Nos. WO 98/23289; WO 2009/058492; and WO 2010/033279, which are herein incorporated by reference in their entireties. 
     In some embodiments, the Fc Domain-containing binding molecules of the present invention exhibiting enhanced half-life possess a variant Fc Domain comprising substitutions at two or more of Fc Domain residues 250, 252, 254, 256, 257, 288, 307, 308, 309, 311, 378, 428, 433, 434, 435 and 436. In particular, two or more substitutions selected from: T250Q, M252Y, S254T, T256E, K288D, T307Q, V308P, A378V, M428L, N434A, H435K, and Y436I, wherein the numbering is that of the EU index as in Kabat. In a specific embodiment, such molecules may possess a variant IgG Fc Domain comprising the substitution:
         (A) M252Y, S254T and T256E;   (B) M252Y and S254T;   (C) M252Y and T256E;   (D) T250Q and M428L;   (E) T307Q and N434A;   (F) A378V and N434A;   (G) N434A and Y436I;   (H) V308P and N434A; or   (I) K288D and H435K.       

     In a preferred embodiment, an Fc Domain-containing CD16 x DA Binding Molecule of the present invention possesses a variant IgG Fc Region comprising any 1, 2, or 3 of the substitutions: M252Y, S254T and T256E. The invention further encompasses CD16 x DA Binding Molecules possessing variant Fc Regions comprising:
         (A) one or more mutations which alter effector function and/or FcγR; and   (B) one or more mutations which extend serum half-life.       

     An IgG1 sequence for the CH2 and CH3 Domains of the Fc Domain-containing molecules of the present invention that provides an increased half-life (and that has a 10-fold increase in binding to both cynomolgus monkey and human FcRn) (Dall&#39;Acqua, W. F. et al. (2006) “ Properties of Human IgG 1 s Engineered for Enhanced Binding to the Neonatal Fc Receptor  ( FcRn ),” J. Biol. Chem. 281(33):23514-23524) will comprise the substitutions M252Y/S254T/T256E (SEQ ID NO:48): 
     
       
         
           
               
               
            
               
                   
                 APELLGGPSV FLFPPKPKDT L   Y   I   T   R   E   PEVT CVVVDVSHED 
               
               
                   
               
               
                   
                 PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 
               
               
                   
               
               
                   
                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 
               
               
                   
               
               
                   
                 LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN 
               
               
                   
               
               
                   
                 YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE 
               
               
                   
               
               
                   
                 ALHNHYTQKS LSLSPG   X     
               
            
           
         
       
     
     wherein  X  is lysine (K) or is absent. 
     An alternative IgG1 sequence for the CH2 and CH3 Domains of the Fc Domain-containing molecules of the present invention combining the reduced or abolished effector function provided by the substitutions L234A/L235A and the increased serum half-life provided by the substitutions M252Y/S254T/T256E is provided by SEQ ID NO: 49: 
     
       
         
           
               
               
            
               
                   
                 APE   AA   GGPSV FLFPPKPKDT L   Y   I   T   R   E   PEVT CVVVDVSHED  
               
               
                   
               
               
                   
                 PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 
               
               
                   
               
               
                   
                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 
               
               
                   
               
               
                   
                 LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN 
               
               
                   
               
               
                   
                 YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE 
               
               
                   
               
               
                   
                 ALHNHYTQKS LSLSPG   X     
               
            
           
         
       
     
     wherein  X  is lysine (K) or is absent. 
     A further preferred IgG1 sequence for the CH2 and CH3 Domains of the Fc Region-containing molecules of the present invention comprises the L234A/L235A substitutions (shown underlined) that reduce or abolish effector function and the M252Y, S254T and T256E substitutions (shown underlined), so as to extend the serum half-life and the S442C substitution (shown underlined), so as to permit two CH3 domains to be covalently bonded to one another via a disulfide bond or to permit conjugation of a drug moiety. The amino acid sequence of such molecule is (SEQ ID NO:50): 
                            APE   AA   GGPSV FLFPPKPKDT L   Y   I   T   R   E   PEVT CVVVDVSHED                   PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH                   QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT                   LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN                   YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE                   ALHNHYTQKS L   C   LSPG   X                
wherein  X  is lysine (K) or is absent.
 
     For certain antibodies, diabodies and trivalent binding molecules that are desired to have Fc-Domain-containing polypeptide chains of differing amino acid sequence (e.g., whose Fc Domain-containing polypeptide chains are desired to not be identical), it is desirable to reduce or prevent homodimerization from occurring between the CH2-CH3 Domains of identical chains (e.g., two first polypeptide chains or between the CH2-CH3 Domains of two third polypeptide chains). The CH2 and/or CH3 Domains of such polypeptide chains need not be identical in sequence, and advantageously are modified to foster heterodimer complexing between the two polypeptide chains. For example, an amino acid substitution (preferably a substitution with an amino acid comprising a bulky side group forming a “knob”, e.g., tryptophan) can be introduced into the CH2 or CH3 Domain such that steric interference will prevent interaction with a similarly mutated domain and will obligate the mutated domain to pair with a domain into which a complementary, or accommodating mutation has been engineered, i.e., “the hole” (e.g., a substitution with glycine). Such sets of mutations can be engineered into any pair of polypeptides comprising CH2-CH3 Domains that forms an Fc Domain to foster heterodimerization. Methods of protein engineering to favor heterodimerization over homodimerization are well-known in the art, in particular with respect to the engineering of immunoglobulin-like molecules, and are encompassed herein (see e.g., Ridgway et al. (1996) “‘ Knobs - Into - Holes’ Engineering Of Antibody CH 3  Domains For Heavy Chain Heterodimerization ,” Protein Engr. 9:617-621, Atwell et al. (1997) “ Stable Heterodimers From Remodeling The Domain Interface Of A Homodimer Using A Phage Display Library ,” J. Mol. Biol. 270: 26-35, and Xie et al. (2005) “ A New Format Of Bispecific Antibody: Highly Efficient Heterodimerization, Expression And Tumor Cell Lysis ,” J. Immunol. Methods 296:95-101; each of which is hereby incorporated herein by reference in its entirety). 
     A preferred knob is created by modifying an IgG Fc Domain to contain the modification T366W. A preferred hole is created by modifying an IgG Fc Domain to contain the modification T366S, L368A and Y407V. To aid in purifying a hole-bearing polypeptide chain homodimer from the final bispecific heterodimeric Fc Domain-containing molecule, the Protein A Binding Domain of the hole-bearing CH2 and CH3 Domains of a polypeptide chain is preferably mutated by amino acid substitution at position 435 (H435R). Thus, the hole-bearing polypeptide chain homodimer will not bind protein A, whereas the bispecific heterodimer will retain its ability to bind protein A via the Protein A Binding Domain. In an alternative embodiment, the hole-bearing polypeptide chain may incorporate amino acid substitutions at positions 434 and 435 (N434A/N435K). 
     A preferred IgG1 amino acid sequence for the CH2 and CH3 Domains of one Fc Domain-containing polypeptide chain of an Fc Domain-containing molecule of the present invention will have the “knob-bearing” sequence (SEQ ID NO:51): 
     
       
         
           
               
               
            
               
                   
                 APE   AA   GGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED 
               
               
                   
               
               
                   
                 PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 
               
               
                   
               
               
                   
                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 
               
               
                   
               
               
                   
                 LPPSREEMTK NQVSL   W   CLVK GFYPSDIAVE WESNGQPENN 
               
               
                   
               
               
                   
                 YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE 
               
               
                   
               
               
                   
                 ALHNHYTQKS LSLSPG   X     
               
            
           
         
       
     
     wherein  X  is lysine (K) or is absent. 
     An alternative IgG1 amino acid sequence for the CH2 and CH3 Domains of one Fc Domain-containing polypeptide chain of an Fc Domain-containing molecule of the present invention having a M252Y/S254T/T256E substitution and a “knob-bearing” sequence is SEQ ID NO:52: 
     
       
         
           
               
               
            
               
                   
                 APE   AA   GGPSV FLFPPKPKDT L   Y   I   T   R   E   PEVT CVVVDVSHED 
               
               
                   
               
               
                   
                 PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 
               
               
                   
               
               
                   
                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 
               
               
                   
               
               
                   
                 LPPSREEMTK NQVSL   W   CLVK GFYPSDIAVE WESNGQPENN 
               
               
                   
               
               
                   
                 YKTTPPVLDS DGSFFLYSKL TVDKSTWQQG NVFSCSVMHE 
               
               
                   
               
               
                   
                 ALHNHYTQKS LSLSPG   X     
               
            
           
         
       
     
     wherein  X  is lysine (K) or is absent. 
     A preferred IgG1 amino acid sequence for the CH2 and CH3 Domains of the other Fc Domain-containing polypeptide chain of an Fc Domain-containing molecule of the present invention having two polypeptide chains (or the third polypeptide chain of an Fc Domain-containing molecule having three, four, or five polypeptide chains) will have the “hole-bearing” sequence (SEQ ID NO:53): 
     
       
         
           
               
               
            
               
                   
                 APE   AA   GGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED 
               
               
                   
               
               
                   
                 PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 
               
               
                   
               
               
                   
                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 
               
               
                   
               
               
                   
                 LPPSREEMTK NQV   S   LSC   A   VK GFYPSDIAVE WESNGQPENN 
               
               
                   
               
               
                   
                 YKTTPPVLDS DGSFFL   V   SKL TVDKSRWQQG NVFSCSVMHE 
               
               
                   
               
               
                   
                 ALHN   R   YTQKS LSLSPG   X     
               
            
           
         
       
     
     wherein  X  is lysine (K) or is absent. 
     An alternative IgG1 amino acid sequence for the CH2 and CH3 Domains of the other Fc Domain-containing polypeptide chain of an Fc Domain-containing molecule of the present invention having a M252Y/S254T/T256E substitution and a “hole-bearing” sequence is SEQ ID NO:54: 
     
       
         
           
               
               
            
               
                   
                 APE   AA   GGPSV FLFPPKPKDT L   Y   I   T   R   E   PEVT CVVVDVSHED 
               
               
                   
               
               
                   
                 PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 
               
               
                   
               
               
                   
                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 
               
               
                   
               
               
                   
                 LPPSREEMTK NQV   S   LSC   A   VK GFYPSDIAVE WESNGQPENN 
               
               
                   
               
               
                   
                 YKTTPPVLDS DGSFFL V SKL TVDKSRWQQG NVFSCSVMHE 
               
               
                   
               
               
                   
                 ALHNRYTQKS LSLSPG   X     
               
            
           
         
       
     
     wherein  X  is lysine (K) or is absent. 
     As will be noted, the CH2-CH3 Domains of SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, and SEQ ID NO:54 include a substitution at position 234 with alanine and 235 with alanine, and thus form an Fc Domain exhibit decreased (or substantially no) binding FcγRIA (CD64), FcγRIIA (CD32A), FcγRIIB (CD32B), FcγRIIIA (CD16a) or FcγRIIIB (CD16b) (relative to the binding exhibited by the wild-type Fc Domain (SEQ ID NO:12). The invention also encompasses such CH2-CH3 Domains, which comprise the wild-type alanine residues, alternative and/or additional substitutions which modify effector function and/or FγR binding activity of the Fc Domain. The invention also encompasses such CH2-CH3 Domains, which further comprise one or more half-live extending amino acid substitutions. In particular, the invention encompasses such hole-bearing and such knob-bearing CH2-CH3 Domains which further comprise the M252Y/S254T/T256E. 
     An IgG4 amino acid sequence for the CH2 and CH3 Domains of the one Fc Domain-containing polypeptide chain of an Fc Domain-containing molecule of the present invention has enhanced serum half-life (relative to IgG1 CH2 and CH3 Domains) due to its possession of Y252/T254/E256 (SEQ ID NO:55): 
     
       
         
           
               
               
            
               
                   
                 APEFLGGPSV FLFPPKPKDT L   Y   I   T   R   E   PEVT CVVVDVSQED 
               
               
                   
                   
               
               
                   
                 PEVQFNWYVD GVEVHNAKTK PREEQFNSTY RVVSVLTVLH 
               
               
                   
                   
               
               
                   
                 QDWLNGKEYK CKVSNKGLPS SIEKTISKAK GQPREPQVYT 
               
               
                   
                   
               
               
                   
                 LPPSQEEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN 
               
               
                   
                   
               
               
                   
                 YKTTPPVLDS DGSFFLYSRL TVDKSRWQEG NVFSCSVMHE 
               
               
                   
                   
               
               
                   
                 ALHNHYTQKS LSLSLG   X     
               
            
           
         
       
     
     wherein  X  is lysine (K) or is absent. 
     A “knob-bearing” variant of such an IgG4 CH2-CH3 amino acid sequence has the amino acid sequence of SEQ ID NO:56: 
     
       
         
           
               
               
            
               
                   
                 APEFLGGPSV FLFPPKPKDT L   Y   I   T   R   E   PEVT CVVVDVSQED 
               
               
                   
                   
               
               
                   
                 PEVQFNWYVD GVEVHNAKTK PREEQFNSTY RVVSVLTVLH 
               
               
                   
                   
               
               
                   
                 QDWLNGKEYK CKVSNKGLPS SIEKTISKAK GQPREPQVYT 
               
               
                   
                   
               
               
                   
                 LPPSQEEMTK NQVSL   W   CLVK GFYPSDIAVE WESNGQPENN 
               
               
                   
                   
               
               
                   
                 YKTTPPVLDS DGSFFLYSRL TVDKSRWQEG NVFSCSVMHE 
               
               
                   
                   
               
               
                   
                 ALHNHYTQKS LSLSLG   X     
               
            
           
         
       
     
     wherein  X  is lysine (K) or is absent. 
     A “hole-bearing” variant of such an IgG4 CH2-CH3 amino acid sequence has the amino acid sequence of SEQ ID NO:57: 
     
       
         
           
               
               
            
               
                   
                 APEFLGGPSV FLFPPKPKDT L   Y   I   T   R   E   PEVT CVVVDVSQED 
               
               
                   
                   
               
               
                   
                 PEVQFNWYVD GVEVHNAKTK PREEQFNSTY RVVSVLTVLH 
               
               
                   
                   
               
               
                   
                 QDWLNGKEYK CKVSNKGLPS SIEKTISKAK GQPREPQVYT 
               
               
                   
                   
               
               
                   
                 LPPSQEEMTK NQVSL   S   CA   V   K GFYPSDIAVE WESNGQPENN 
               
               
                   
                   
               
               
                   
                 YKTTPPVLDS DGSFFL   V   SRL TVDKSRWQEG NVFSCSVMHE 
               
               
                   
                   
               
               
                   
                 ALHN   R   YTQKS LSLSLG   X     
               
            
           
         
       
     
     wherein  X  is lysine (K) or is absent. 
     It is preferred that the first polypeptide chain will have a “knob-bearing” CH2-CH3 sequence, such as that of SEQ ID NO:51 or SEQ ID NO:52. However, as will be recognized, a “hole-bearing” CH2-CH3 Domain (e.g., SEQ ID NO:53 or SEQ ID NO:54) could be employed in the first polypeptide chain, in which case, a “knob-bearing” CH2-CH3 Domain (e.g., SEQ ID NO:51 or SEQ ID NO:52) would be employed in the second polypeptide chain of an Fc Domain-containing molecule of the present invention having two polypeptide chains (or in the third polypeptide chain of an Fc Domain-containing molecule having three, four, or five polypeptide chains). 
     In other embodiments, the invention encompasses Fc Domain-containing binding molecules comprising CH2 and/or CH3 Domains that have been engineered to favor heterodimerization over homodimerization using mutations known in the art, such as those disclosed in PCT Publication No. WO 2007/110205; WO 2011/143545; WO 2012/058768; WO 2013/06867, all of which are incorporated herein by reference in their entirety. 
     III. Trivalent Binding Molecules Containing Fc Domains 
     A further embodiment of the present invention relates to trivalent binding molecules comprising an Fc Domain capable of simultaneously binding a First Epitope, a Second Epitope and a Third Epitope, wherein at least one of such epitopes is not identical to another. Such trivalent binding molecules comprise three Epitope Binding Domains, two of which are Diabody-Type Binding Domains, which provide Binding Domain A and Binding Domain B, and one of which is a Fab-Type Binding Domain, or an scFv-Type Binding Domain, which provides Binding Domain C (see, e.g.,  FIGS. 6A-6H , PCT Publication Nos. WO 2015/184207 and WO 2015/184203). Such trivalent binding molecules thus comprise “VL1”/“V111” domains that are capable of binding the First Epitope and “VL2”/“VH2” domains that are capable of binding the Second Epitope and “VL3” and “VH3” domains that are capable of binding the “third” epitope of such trivalent binding molecule. A “Diabody-Type Binding Domain” is the type of Epitope Binding Domain present in a diabody, as described above. Each of a “Fab-Type Binding Domain” and an “scFv-Type Binding Domain” are Epitope Binding Domains that are formed by the interaction of the VL Domain of an immunoglobulin Light Chain and a complementing VH Domain of an immunoglobulin Heavy Chain. Fab-Type Binding Domains differ from Diabody-Type Binding Domains in that the two polypeptide chains that form a Fab-Type Binding Domain comprise only a single Epitope Binding Domain, whereas the two polypeptide chains that form a Diabody-Type Binding Domain comprise at least two Epitope Binding Domains. Similarly, scFv-Type Binding Domains also differ from Diabody-Type Binding Domains in that they comprise only a single Epitope Binding Domain. Thus, as used herein Fab-Type, and scFv-Type Binding Domains are distinct from Diabody-Type Binding Domains. 
     Typically, the trivalent Binding Molecules of the present invention will comprise four different polypeptide chains (see  FIGS. 6A-6B ), however, the molecules may comprise fewer or greater numbers of polypeptide chains, for example by fusing such polypeptide chains to one another (e.g., via a peptide bond) or by dividing such polypeptide chains to form additional polypeptide chains, or by associating fewer or additional polypeptide chains via disulfide bonds.  FIGS. 6E-6F  illustrate this aspect of the present invention by schematically depicting such molecules having three polypeptide chains. As provided in  FIGS. 6A-611 , the trivalent binding molecules of the present invention may have alternative orientations in which the Diabody-Type Binding Domains are N-terminal ( FIGS. 6A, 6B, 6C, 6E and 6F ) or C-terminal ( FIGS. 6D, 6G and 6H ) to an Fc Domain. CH2 and CH3 Domains useful for the generation of trivalent binding molecules are provided above and include knob-bearing and hole-bearing domains. 
     In certain embodiments, the first polypeptide chain of such trivalent binding molecules of the present invention contains: (i) a VL1-containing Domain, (ii) a VH2-containing Domain, (iii) a Heterodimer-Promoting Domain, and (iv) a Domain containing a CH2-CH3 sequence. The VL1 and VL2 Domains are located N-terminal or C-terminal to the CH2-CH3-containing domain as presented in Table 4 (also see,  FIGS. 6A and 6B ). The second polypeptide chain of such embodiments contains: (i) a VL2-containing Domain, (ii) a VH1-containing Domain, and (iii) a Heterodimer-Promoting Domain. The third polypeptide chain of such embodiments contains: (i) a VH3-containing Domain, (ii) a CH1-containing Domain and (iii) a Domain containing a CH2-CH3 sequence. The third polypeptide chain may be the Heavy Chain of an antibody that contains a VH3 and a Heavy Chain constant region, or a polypeptide that contains such domains. The fourth polypeptide of such embodiments contains: (i) a VL3-containing Domain and (ii) a CL-containing Domain. The fourth polypeptide chains may be a Light Chain of an antibody that contains a VL3 complementary to the VH3 of the third polypeptide chain, or a polypeptide that contains such domains. The third or fourth polypeptide chains may be isolated from naturally occurring antibodies. Alternatively, they may be constructed recombinantly, synthetically or by other means. 
     The Light Chain Variable Domain of the first and second polypeptide chains are separated from the Heavy Chain Variable Domains of such polypeptide chains by an intervening spacer peptide having a length that is too short to permit their VL1/VH2 (or their VL2/VH1) domains to associate together to form an Epitope Binding Domain capable of binding either the First or Second Epitope. A preferred intervening spacer peptide (Linker 1) for this purpose has the sequence (SEQ ID NO:16): 
                            GGGSGGGG.            
Other Domains of the trivalent binding molecules may be separated by one or more intervening spacer peptides (Linkers), optionally comprising a cysteine residue. In particular, as provided above, such Linkers will typically be incorporated between Variable Domains (i.e., VH or VL) and peptide Heterodimer-Promoting Domains (e.g., an E-coil or K-coil) and between such peptide Heterodimer-Promoting Domains (e.g., an E-coil or K-coil) and CH2-CH3 Domains. Exemplary linkers useful for the generation of trivalent binding molecules are provided above and are also provided in PCT Application Nos: PCT/US15/33081; and PCT/US15/33076. Thus, the first and second polypeptide chains of such trivalent binding molecules associate together to form a VL1/VH1 Binding Domain capable of binding a First Epitope, as well as a VL2/VH2 Binding Domain that is capable of binding a Second Epitope. The third and fourth polypeptide chains of such trivalent binding molecules associate together to form a VL3/VH3 Binding Domain that is capable of binding a Third Epitope.
 
     As described above, the trivalent binding molecules of the present invention may comprise three polypeptides. Trivalent binding molecules comprising three polypeptide chains may be obtained by linking the domains of the fourth polypeptide N-terminal to the VH3-containing Domain of the third polypeptide (e.g., using an intervening spacer peptide (Linker 4)). Alternatively, a third polypeptide chain of a trivalent binding molecule of the invention containing the following domains is utilized: (i) a VL3-containing Domain, (ii) a VH3-containing Domain, and (iii) a Domain containing a CH2-CH3 sequence, wherein the VL3 and VH3 are spaced apart from one another by an intervening spacer peptide that is sufficiently long (at least 9 or more amino acid residues) so as to allow the association of these domains to form an Epitope Binding Domain. One preferred intervening spacer peptide for this purpose has the sequence: 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 41) 
               
               
                   
                 GGGGSGGGGSGGGGS. 
               
            
           
         
       
     
     It will be understood that the VL1/VH1, VL2/VH2, and VL3/VH3 Domains of such trivalent binding molecules may be different so as to permit binding that is mono-specific, bispecific or trispecific. In particular, the VL and VH Domains may be selected such that a trivalent binding molecule comprises two Binding Domains for a First Epitope and one Binding Domains for a Second Epitope, or one Binding Domain for a First Epitope and two Binding Domains for a Second Epitope, or one Binding Domain for a First Epitope, one Binding Domain for a Second Epitope and one Binding Domain for a Third Epitope. 
     The general structure of the polypeptide chains of representative trivalent binding molecules of invention is provided in  FIGS. 6A-6H  and in Table 5: 
     
       
         
           
               
               
               
             
               
                 TABLE 5 
               
               
                   
               
             
            
               
                 Four Chain 
                 2 nd   Chain 
                 NH 2 -VL2-VH1-HPD-COOH 
               
               
                 1 st   
                 1 st   Chain  
                 NH 2 -VL1-VH2-HPD-CH2-CH3-COOH 
               
               
                 Orientation 
                 3 rd  Chain  
                 NH 2 -VH3-CH1-CH2-CH3-COOH 
               
               
                   
                 2 nd  Chain 
                 NH 2 -VL3-CL-COOH 
               
               
                 Four Chain 
                 2 nd  Chain 
                 NH 2 -VL2-VH1-HPD-COOH 
               
               
                 2nd 
                 1 st  Chain  
                 NH 2 -CH2-CH3-VL1-VH2-HPD-COOH 
               
               
                 Orientation 
                 3 rd  Chain  
                 NH 2 -VH3-CH1-CH2-CH3-COOH 
               
               
                   
                 2 nd  Chain 
                 NH 2 -VL3-CL-COOH 
               
               
                 Three Chain 
                 2 nd   Chain 
                 NH 2 -VL2-VH1-HPD-COOH 
               
               
                 1st 
                 1 st  Chain  
                 NH 2 -VL1-VH2-HPD-CH2-CH3-COOH 
               
               
                 Orientation 
                 3 rd  Chain  
                 NH 2 -VL3-VH3-HPD-CH2-CH3-COOH 
               
               
                 Three Chain 
                 2 nd  Chain 
                 NH 2 -VL2-VH1-HPD-COOH 
               
               
                 2 nd    
                 1 st   Chain  
                 NH 2 -CH2-CH3-VL1-VH2-HPD-COOH 
               
               
                 Orientation 
                 3 rd  Chain  
                 NH 2 -VL3-VH3-HPD-CH2-CH3-COOH 
               
               
                   
               
               
                 HPD = Heterodimer-Promoting Domain 
               
            
           
         
       
     
     As provided above, such trivalent binding molecules may comprise three, four, five, or more polypeptide chains. 
     IV. Embodiments of the Invention 
     As stated above, the present invention is directed to a CD16 x DA Binding Molecule (e.g., an antibody, a diabody, an scFv, an antibody, a TandAb, etc.) comprising a Binding Domain capable of binding an epitope of CD16 (i.e., a “CD16-Binding Domain”) and a Binding Domain capable of binding an epitope of a Disease Antigen (i.e., a “Disease Antigen-Binding Domain”). The invention thus encompasses binding molecules comprising one or more of the VH and/or VL Domains of an antibody that binds to CD16, or more preferably, the CDR H 1, CDR H 2, and CDR H 3, and the CDR L 1, CDR L 2 and CDR L 3 portions of such Domains. In a preferred embodiment of the invention, such binding molecules will additionally contain Binding Domains sufficient to permit such molecules to bind to epitopes of one, two, three or or more than three Disease Antigens. The present invention is also directed to pharmaceutical compositions that comprise such molecule(s). 
     By possessing Binding Domains sufficient to immunospecifically bind CD16 and a Disease Antigen, the molecules of the present invention have the ability to co-localize CD16-expressing cells (and especially Natural Killer cells) to the site(s) of cells expressing the Disease Antigen so as to enhance the likelihood of ADCC-mediated killing of the target cell. As discussed above, such molecules may be bispecific, or may be capable of binding more than two epitopes. 
     In one embodiment, such CD16-binding molecules of the present invention will be mono-specific so as to possess the ability to bind to only a single epitope of CD16 and only a single epitope of a Disease Antigen. 
     Alternatively, such molecules may be multi-specific, i.e., capable of binding one, two, three or four total epitopes, which may be apportioned in any manner to bind one, two or three epitope(s) of CD16 (which two or three CD16 epitopes may be the same or different) and three, two or one epitope(s) of one or more Disease Antigen(s). 
     Thus, where such molecules are capable of immunospecifically binding to only a single Disease Antigen, they may be capable of immunospecifically binding to only one CD16 epitope and to one, two or three epitope(s) of the Disease Antigen (which two Disease Antigen epitopes may be the same or different, and which three epitopes may be the same, or may be different, or may be two epitopes that are the same and one epitope that is different), or they may be capable of immunospecifically binding to only two CD16 epitopes (which two epitopes may be the same or different) and one or two epitope(s) of the Disease Antigen (which two Disease Antigen epitopes may be the same or different), or they may be capable of immunospecifically binding to three CD16 epitopes (which three epitopes may be the same, or may be different or may be two epitopes that are the same and one epitope that is different) and 1 epitope of the Disease Antigen. 
     Similarly, where such molecules are capable of immunospecifically binding to two different Disease Antigens (e.g., a First Disease Antigen and a Second Disease Antigen), they may be capable of immunospecifically binding to only one CD16 epitope and to one or two epitope(s) of the First Disease Antigen (which two First Disease Antigen epitopes may be the same or different) and two or one epitope(s) of the Second Disease Antigen (which two Second Disease Antigen epitopes may be the same or different), or they may be capable of immunospecifically binding to only two CD16 epitopes (which two epitopes may be the same or different) and one epitope of the First Disease Antigen and one epitope of the Second Disease Antigen. 
     Similarly, such molecules may be capable of immunospecifically binding to three different Disease Antigens (e.g., a First Disease Antigen, a Second Disease Antigen and a Third Disease Antigen) and only one CD16 epitope. 
     Thus, for example, such molecules may bind:
         (1) a single epitope of CD16 and a single epitope of a Disease Antigen that is arrayed on the surface of the target cell;   (2) a single epitope of CD16 and two epitopes of a Disease Antigen that is arrayed on the surface of the target cell   (3) a single epitope of CD16 and three epitopes of a Disease Antigen that is arrayed on the surface of the target cell;   (4) a single epitope of CD16, one epitope of a First Disease Antigen that is arrayed on the surface of the target cell, and one epitope of a Second Disease Antigen that is arrayed on the surface of the target cell;   (5) a single epitope of CD16, two epitopes of a First Disease Antigen that is arrayed on the surface of the target cell, and one epitope of a Second Disease Antigen that is arrayed on the surface of the target cell;   (6) two epitopes of CD16 and a single epitope of a Disease Antigen that is arrayed on the surface of the target cell;   (7) two epitopes of CD16 and two epitopes of a Disease Antigen that is arrayed on the surface of the target cell;   (8) two epitopes of CD16 and one epitope of a First Disease Antigen that is arrayed on the surface of the target cell and one epitope of a Second Disease Antigen that is arrayed on the surface of the target cell;   (9) three epitopes of CD16 and one epitope of a Disease Antigen that is arrayed on the surface of the target cell;   in all cases in which binding is to more than one epitope of CD16 or a Disease Antigen, such epitopes may be the same or may be different or may be the same as one such epitope and different from another such epitope.       

     Table 6 illustrates possible combination binding specificities of exemplary molecules of the invention. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Number of Epitopes Recognized by Exemplary CD16 × DA 
               
               
                 Binding Molecules of the Invention Possessing  
               
               
                 Two, Three or Four Epitope Binding Domains That Are 
               
               
                 Capable of Mediating the Redirected Killing of a Target Cell 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                 Number  
                 Number  
                 Number  
               
               
                   
                   
                 Number  
                 of 
                 of 
                 of 
               
               
                 Total 
                   
                 of 
                 Epitopes 
                 Epitopes 
                 Epitopes 
               
               
                 Number  
                 Number  
                 Epitopes  
                 of 
                 of 
                 of 
               
               
                 of 
                 of 
                 of 
                 2 nd    
                 3 rd    
                 Non-CD16 
               
               
                 Binding 
                 CD16 
                 1 st  Disease 
                 Disease 
                 Disease 
                 Cell Surface 
               
               
                 Domains 
                 Epitope(s) 
                 Antigen 
                 Antigen 
                 Antigen 
                 Molecule 
               
               
                   
               
               
                 2 
                 1 
                 1 
                 0 
                 0 
                 0 
               
               
                 3 
                 1 
                 1 
                 1 
                 0 
                 0 
               
               
                 3 
                 1 
                 1 
                 0 
                 0 
                 1 
               
               
                 3 
                 1 
                 2 
                 0 
                 0 
                 0 
               
               
                 3 
                 2 
                 1 
                 0 
                 0 
                 0 
               
               
                 4 
                 1 
                 1 
                 1 
                 0 
                 1 
               
               
                 4 
                 1 
                 1 
                 1 
                 1 
                 0 
               
               
                 4 
                 1 
                 2 
                 0 
                 0 
                 1 
               
               
                 4 
                 1 
                 2 
                 1 
                 0 
                 0 
               
               
                 4 
                 2 
                 1 
                 1 
                 0 
                 0 
               
               
                 4 
                 2 
                 1 
                 0 
                 0 
                 1 
               
               
                   
               
            
           
         
       
     
     By forming more complex molecules, one may obtain CD16-binding molecules that are capable of binding one or more Disease Antigens and that possess more than four epitope binding domains. Thus, no limitation is placed on the nature of epitopes or additional epitopes that may be bound by the molecules of the present invention other than that such additional binding capability does not prevent the molecule or Binding Domain thereof that is capable of binding to an epitope of CD16 from such binding and does not prevent the molecule or Binding Domain thereof that is capable of binding to an epitope of a Disease Antigen from such binding. Thus, the CD16 Binding molecules of the present invention may possess Epitope Binding Domains alternative or additional Epitope Binding Domains. As an example, the invention contemplates a binding molecule that comprises a First Epitope Binding Domain capable of immunospecifically binding an epitope of CD16 and a Second Epitope Binding Domain that is capable of immunospecifically binding an epitope of a Disease Antigen that is arrayed on the surface of such target cell and a Third Epitope Binding Domain capable of immunospecifically binding a different cell surface molecule, such as a non-CD16 cell surface molecule of a Natural Killer cell. 
     V. Exemplary Binding Molecules 
     The present invention is directed molecules (e.g., an antibody, a diabody, an scFv, an antibody, a TandAb, etc.) capable of binding human CD16 by virtue of their possession of a CD16 Binding Domain. The present invention is particularly directed to such CD-16 Binding Molecules that are CD16 x DA Binding Molecules. Listed below are exemplary antibodies that may be used to produce the binding molecules and combination therapy of the present invention. 
     A. Exemplary Anti-Human CD16 Antibodies 
     1. CD16-M1 and CD16-M2 and Their Humanized Derivatives hCD16-M1 and hCD16-M2 
     The present invention provides murine anti-human CD16 monoclonal antibodies: CD16-M1 and CD16-M2, and their humanized derivatives: hCD16-M1 and hCD16-M2, which are novel, high affinity anti-human CD16 monoclonal antibodies that bind well to both the CD16 158F allotype and the CD16 158V allotype, and that bind CD16 at a site that does not block CD16-IgG binding. Such antibodies are particularly preferred for the purposes of the present invention since they can be readily employed in a patient population irrespective of its 158F/158V CD16A polymorphisms. 
     (a) Anti-Human CD16 Monoclonal Antibody CD16-M1 
     The amino acid sequence of the VH Domain of murine anti-human CD16 monoclonal antibody CD16-M1 (SEQ ID NO:64) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVKLVESGGT LVKPGGSLKL SCAASGFTFN    NYGMS   WVRQT 
               
               
                   
                   
               
               
                   
                 PEKRLEWVA   T       ISGGGSYTFY       PDSVKG   RFTI SRDNAKNSLY 
               
               
                   
                   
               
               
                   
                 LQMSSLRSED TALYYCIR   QS       ARAPEPY   WGQ GTLVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of murine anti-human CD16 monoclonal antibody CD16-M1 (SEQ ID NO:65) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIVMTQSQKF MSTSVGDRVS VTC   KASQNVG       THVA   WYQQKS 
               
               
                   
                   
               
               
                   
                 GQSPKSLLY   S       ASYRYS   GVPD RFSGSGSGTD FTLTISNVQS 
               
               
                   
                   
               
               
                   
                 EDLAEYFC   QQ       YKSYPLT   FGA GTKLELK 
               
            
           
         
       
     
     The CDRs of anti-human CD16 monoclonal antibody CD16-M1 are shown in Table 7. 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 CDRs of Anti-Human CD16-Monoclonal Antibody 
               
               
                 CD16-M1 
               
            
           
           
               
               
               
            
               
                 CDR 
                 Sequence 
                 SEQ ID NO 
               
               
                   
               
               
                 CDR H 1 
                 
                   NYGMS 
                 
                 SEQ ID NO: 66 
               
               
                   
               
               
                 CDR H 2 
                 
                   TISGGGSYTFYPDSVKG 
                 
                 SEQ ID NO: 67 
               
               
                   
               
               
                 CDR H 3 
                 
                   QSARAPEPY 
                 
                 SEQ ID NO: 68 
               
               
                   
               
               
                 CDR L 1 
                 
                   KASQNVGTHVA 
                 
                 SEQ ID NO: 69 
               
               
                   
               
               
                 CDR L 2 
                 
                   SASYRYS 
                 
                 SEQ ID NO: 70 
               
               
                   
               
               
                 CDR L 3 
                 
                   QQYKSYPLT 
                 
                 SEQ ID NO: 71 
               
               
                   
               
            
           
         
       
     
     hCD16-M1 is a humanized derivative of murine anti-human CD16 monoclonal antibody CD16-M1. The amino acid sequence of the VH Domain of hCD16-M1 (SEQ ID NO:72) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLVESGGG LVKPGGSLRL SCAASGFTFS    NYGMS   WVRQA 
               
               
                   
                   
               
               
                   
                 PGKGLEWVA   T       ISGGGSYTFY       PDSVKG   RFTI SRDNAKNSLY 
               
               
                   
                   
               
               
                   
                 LQMNSLRTED TALYYCVR   QS       ARAPEPY   WGQ GTLVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of hCD16-M1 (SEQ ID NO:73) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSF LSASVGDRVT ITC   RASQNVG       THVA   WYQQKP 
               
               
                   
                   
               
               
                   
                 GKAPKSLLY   S       ASYRYS   GVPS RFSGSGSGTD FTLTISSLQS 
               
               
                   
                   
               
               
                   
                 EDIATYYC   QQ       YKSYPLT   FGQ GTKLEIK 
               
            
           
         
       
     
     hCD16-M1A is an optimized derivative of the humanized anti-human CD16 monoclonal antibody hCD16-M1. hCD16-M1A comprises the VL Domain of hCD16-M1 (SEQ ID NO:73) and an optimized VH Domain comprising mutations in CDR H 3. The amino acid sequence of the optimized VH Domain of hCD16-M1A (SEQ ID NO:58) is shown below (the mutated CDR H 3 residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLVESGGG LVKPGGSLRL SCAASGFTFS NYGMSWVRQA 
               
               
                   
                   
               
               
                   
                 PGKGLEWVAT ISGGGSYTFY PDSVKGRFTI SRDNAKNSLY 
               
               
                   
                   
               
               
                   
                 LQMNSLRTED TALYYCVRQS A   NS   P   V   PYWGQ GTLVTVSS 
               
            
           
         
       
     
     hCD16-M1B is an optimized derivative of the humanized anti-human CD16 monoclonal antibody hCD16-M1. hCD16-M1B comprises the VH Domain of hCD16-M1 (SEQ ID NO:72) and an optimized VL Domain comprising mutations in CDR L 3. The amino acid sequence of the optimized VL Domain of hCD16-M1B (SEQ ID NO:59) is shown below (the mutated CDR L 3 residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSF LSASVGDRVT ITCRASQNVG THVAWYQQKP 
               
               
                   
                   
               
               
                   
                 GKAPKSLLYS ASYRYSGVPS RFSGSGSGTD FTLTISSLQS 
               
               
                   
                   
               
               
                   
                 EDIATYYCQ   D    Y   TN   YPLTFGQ GTKLEIK 
               
            
           
         
       
     
     hCD16-M1AB is an optimized derivative of the humanized anti-human CD16 monoclonal antibody hCD16-M1. hCD16-M1AB comprises the optimized VH Domain of hCD16-M1A (SEQ ID NO:58) and the optimized VL Domain of hCD16-M1B (SEQ ID NO:59). 
     The CDRs of humanized anti-human CD16 monoclonal antibody hCD16-M1 and the optimized anti-human CD16 monoclonal antibodies hCD16-M1A, hCD16-M1B, hCD16-M1AB are shown in Table 8. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 CDRs of Anti-Human CD16-Monoclonal Antibody 
               
               
                 hCD16-M1 
               
            
           
           
               
               
               
            
               
                 CDR 
                 Sequence 
                 SEQ ID NO 
               
               
                   
               
               
                 CDR H 1 
                 
                   NYGMS 
                 
                 SEQ ID NO: 66 
               
               
                   
               
               
                 CDR H 2 
                 
                   TISGGGSYTFYPDSVKG 
                 
                 SEQ ID NO: 67 
               
               
                   
               
               
                 CDR H 3 
                 
                   QSARAPEPY 
                 
                 SEQ ID NO: 68 
               
               
                   
               
               
                 CDR L 1 
                 
                   RASQNVGTHVA 
                 
                 SEQ ID NO: 74 
               
               
                   
               
               
                 CDR L 2 
                 
                   SASYRYS 
                 
                 SEQ ID NO: 70 
               
               
                   
               
               
                 CDR L 3 
                 
                   QQYKSYPLT 
                 
                 SEQ ID NO: 71 
               
               
                   
               
            
           
           
               
            
               
                 hCD16-M1A 
               
            
           
           
               
               
               
            
               
                 CDR H 1 
                 NYGMS 
                 SEQ ID NO: 66 
               
               
                   
               
               
                 CDR H 2 
                 TISGGGSYTFYPDSVKG 
                 SEQ ID NO: 67 
               
               
                   
               
               
                 CDR H 3 
                 QSA   NS   P   V   PY 
                 SEQ ID NO: 60 
               
               
                   
               
               
                 CDR L 1 
                 RASQNVGTHVA 
                 SEQ ID NO: 74 
               
               
                   
               
               
                 CDR L 2 
                 SASYRYS 
                 SEQ ID NO: 70 
               
               
                   
               
               
                 CDR L 3 
                 QQYKSYPLT 
                 SEQ ID NO: 71 
               
               
                   
               
            
           
           
               
            
               
                 hCD16-M1B 
               
            
           
           
               
               
               
            
               
                 CDR H 1 
                 NYGMS 
                 SEQ ID NO: 66 
               
               
                   
               
               
                 CDR H 2 
                 TISGGGSYTFYPDSVKG 
                 SEQ ID NO: 67 
               
               
                   
               
               
                 CDR H 3 
                 QSARAPEPY 
                 SEQ ID NO: 68 
               
               
                   
               
               
                 CDR L 1 
                 RASQNVGTHVA 
                 SEQ ID NO: 74 
               
               
                   
               
               
                 CDR L 2 
                 SASYRYS 
                 SEQ ID NO: 70 
               
               
                   
               
               
                 CDR L 3 
                 Q   D   Y   TN   YPLT 
                 SEQ ID NO: 61 
               
               
                   
               
            
           
           
               
            
               
                 hCD16-M1AB 
               
            
           
           
               
               
               
            
               
                 CDR H 1 
                 NYGMS 
                 SEQ ID NO: 66 
               
               
                   
               
               
                 CDR H 2 
                 TISGGGSYTFYPDSVKG 
                 SEQ ID NO: 67 
               
               
                   
               
               
                 CDR H 3 
                 QSA   NS   P   V   PY 
                 SEQ ID NO: 60 
               
               
                   
               
               
                 CDR L I 
                 RASQNVGTHVA 
                 SEQ ID NO: 74 
               
               
                   
               
               
                 CDR L 2 
                 SASYRYS 
                 SEQ ID NO: 70 
               
               
                   
               
               
                 CDR L 3 
                 Q   D   Y   TN   YPLT 
                 SEQ ID NO: 61 
               
               
                   
               
            
           
         
       
     
     As will be recognized, CDR L 1 of hCD16-M1, hCD16-M1A, hCD16-M1B, and hCD16-M1AB (RASQNVGTHVA; SEQ ID NO:74) differs from CDR L 1 of CD16-M1 (KASQNVGTHVA; SEQ ID NO:69) in its first residue. Either CDR L 1 may be employed interchangeably, and the present invention encompasses humanized, and optimized CD16 Binding Molecules that comprise a CD16 Epitope Binding Domain having the amino acid sequence of 1, 2 or 3 of the following CDR H s, and/or 1, 2 or 3 of the such CDR L s. 
     (b) Anti-Human CD16 Monoclonal Antibody CD16-M2 
     The amino acid sequence of the VH Domain of murine anti-human CD16 monoclonal antibody CD16-M2 (SEQ ID NO:75) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLQQSGPE LVKPGASVKM SCKASGYTFT    SSAMH   WVKKN 
               
               
                   
                   
               
               
                   
                 PGQGLEWIG   Y       INHYNDGIKY       NERFKG   KATL TSDKSSSTAY 
               
               
                   
                   
               
               
                   
                 MELSSLTSED SAVYYCAT   GY       RYASWFAS   WG QGTLVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of murine anti-human CD16 monoclonal antibody CD16-M2 (SEQ ID NO:76) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DILLTQSPAI LSVSPGERVS FSC   RASQNIG       TSIH   WYQQRT 
               
               
                   
                   
               
               
                   
                 DGSPRLLIK   S       VSESIS   GIPS RFSGSGSGTD FTLTINGVES 
               
               
                   
                   
               
               
                   
                 GDISDYYC   QQ       SNSWPLT   FGA GTKLELK 
               
            
           
         
       
     
     The CDRs of anti-human CD16 monoclonal antibody CD16-M2 are shown in Table 9: 
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 CDRs of Anti-Human CD16 Monoclonal 
               
               
                 Antibody CD16-M2 
               
            
           
           
               
               
               
               
            
               
                   
                 CDR 
                 Sequence 
                 SEQ ID NO 
               
               
                   
               
               
                   
                 CDR H 1 
                 SSAMH 
                 SEQ ID NO: 77 
               
               
                   
               
               
                   
                 CDR H 2 
                 YINHYNDGIKYNERFKG 
                 SEQ ID NO: 78 
               
               
                   
               
               
                   
                 CDR H 3 
                 GYRYASWFAS 
                 SEQ ID NO: 79 
               
               
                   
               
               
                   
                 CDR L 1 
                 RASQNIGTSIH 
                 SEQ ID NO: 80 
               
               
                   
               
               
                   
                 CDR L 2 
                 SVSESIS 
                 SEQ ID NO: 81 
               
               
                   
               
               
                   
                 CDR L 3 
                 QQSNSWPLT 
                 SEQ ID NO: 82 
               
               
                   
               
            
           
         
       
     
     hCD16-M2 is a humanized derivative of murine anti-human CD16 monoclonal antibody CD16-M2. Humanization resulted in two suitable VH Domains (hCD16-M2 VH1 and hCD16-M2 VH2), either of which may be employed with the obtained humanized VL Domain (hCD16-M2 VL1). 
     The amino acid sequence of VH Domain hCD16-M2 VH1 (SEQ ID NO:83) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLVQSGAE VKKPGASVKV SCKASGYTFT 
               
               
                   
               
               
                   
                     SSAMH   WVRQA PGQGLEWMG   Y       INHYNDGIKY     
               
               
                   
               
               
                   
                     NERFKG   RVTI TADKSTSTAY MELSSLRSED 
               
               
                   
               
               
                   
                 TAVYYCAT   GY       RYASWFAS   WG QGTLVTVSS 
               
            
           
         
       
     
     The amino acid sequence of VH Domain hCD16-M2 VH2 (SEQ ID NO:84) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLVQSGAE VKKPGASVKV SCKASGYTFT 
               
               
                   
               
               
                   
                     SSAMH   WVRQA PGQGLEWMG   Y       INHYNDGIKY     
               
               
                   
               
               
                   
                     NERFKG   RVTI TADKSTSTAY MELSSLRSED 
               
               
                   
               
               
                   
                 TAVYYCA     GY       RYASWFAS   WG QGTLVTVSS 
               
            
           
         
       
     
     As will be recognized, the amino acid sequence of hCD16-M2 VH1 (SEQ ID NO:83) differs from that of hCD16-M2 VH2 (SEQ ID NO:84) in possessing a T98R substitution in the residue that immediately precedes CDR H 3 (shown boxed above). 
     The amino acid sequence of the VL Domain hCD16-M2 VL1 (SEQ ID NO:85) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EIVLTQSPAT LSVSPGERAT LSC   RASQNIG     
               
               
                   
               
               
                   
                     TSIH   WYQQKP DQSPKLLIK   S       VSESIS   GVPS 
               
               
                   
               
               
                   
                 RFSGSGSGTD FTLTINSLEA EDFATYYC   QQ     
               
               
                   
               
               
                   
                     SNSWPLT   FGQ GTKLEIK 
               
            
           
         
       
     
     The CDRs of humanized anti-human CD16 monoclonal antibody hCD16-M2 are shown in Table 10. 
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 CDRs of Anti-Human CD16 Monoclonal 
               
               
                 Antibody hCD16-M2 
               
            
           
           
               
               
               
               
            
               
                   
                 CDR 
                 Sequence 
                 SEQ ID NO 
               
               
                   
               
               
                   
                 CDR H 1 
                 SSAMH 
                 SEQ ID NO: 77 
               
               
                   
               
               
                   
                 CDR H 2 
                 YINHYNDGIKYNERFKG 
                 SEQ ID NO: 78 
               
               
                   
               
               
                   
                 CDR H 3 
                 GYRYASWFAS 
                 SEQ ID NO: 79 
               
               
                   
               
               
                   
                 CDR L 1 
                 RASQNIGTSIH 
                 SEQ ID NO: 80 
               
               
                   
               
               
                   
                 CDR L 2 
                 SVSESIS 
                 SEQ ID NO: 81 
               
               
                   
               
               
                   
                 CDR L 3 
                 QQSNSWPLT 
                 SEQ ID NO: 82 
               
               
                   
               
            
           
         
       
     
     B. Exemplary Antibodies that Bind to the Cell Surface of Effector Cells 
     The CD16 x DA Binding Molecules of the present invention, and particularly the trispecific CD16 x DA Binding Molecules of the present invention may comprise a binding site for a non-CD16 cell surface molecule of an effector cell. As used herein, the term “effector cell” denotes a cell that directly or indirectly mediates the killing of target cells (e.g., foreign cells, infected cells or cancer cells). Examples of effector cells include helper T Cells, cytotoxic T Cells, Natural Killer (NK) cells, plasma cells (antibody-secreting B cells), macrophages and granulocytes. Preferred cell surface molecules of such cells include CD2, CD3, CD8, CD16, TCR, and the NKG2D receptor. Accordingly, molecules capable of immunospecifically binding an epitope of such molecules, or to other effector cell surface molecules may be used in accordance with the principles of the present invention. Exemplary antibodies, whose VH and VL Domains may be used to construct molecules capable of mediating the redirected killing of a target cell are provided below. 
     1. Exemplary Anti-NKG2D Antibodies 
     A preferred non-CD16 cell surface molecule of a Natural Killer effector cell is the NKG2D receptor. The NKG2D receptor is expressed on all human (and other mammalian) Natural Killer cells (Bauer, S. et al. (1999) “ Activation Of NK Cells And T Cells By NKG 2 D, A Receptor For Stress - Inducible MICA ,” Science 285(5428):727-729; Jamieson, A. M. et al. (2002) “ The Role Of The NKG 2 D Immunoreceptor In Immune Cell Activation And Natural Killing ,” Immunity 17(1):19-29) as well as on all CD8 +  T cells (Groh, V. et al. (2001) “ Costimulation Of CD 8 αβ T Cells By NKG 2 D Via Engagement By MIC Induced On Virus - Infected Cells ,” Nat. Immunol. 2(3):255-260; Jamieson, A. M. et al. (2002) “ The Role Of The NKG 2 D Immunoreceptor In Immune Cell Activation And Natural Killing ,” Immunity 17(1):19-29). Such binding ligands, and particularly those which are not expressed on normal cells, include the histocompatibility 60 (H60) molecule, the product of the retinoic acid early inducible gene-1 (RAE-1), and the murine UL16-binding proteinlike transcript 1 (MULTI) (Raulet D. H. (2003) “ Roles Of The NKG 2 D Immunoreceptor And Its Ligands ,” Nature Rev. Immunol. 3:781-790; Coudert, J. D. et al. (2005) “ Altered NKG 2 D Function In NK Cells Induced By Chronic Exposure To Altered NKG 2 D Ligand - Expressing Tumor Cells ,” Blood 106:1711-1717). Molecules that specifically bind to the NKG2D Receptor include the anti-NKG2D antibodies “KYK-1.0” and “KYK-2.0” (Kwong, K Y et al. (2008) “ Generation, Affinity Maturation, And Characterization Of A Human Anti - Human NKG 2 D Monoclonal Antibody With Dual Antagonistic And Agonistic Activity ,” J. Mol. Biol. 384:1143-1156). 
     The amino acid sequence of the VH Domain of KYK-1.0 (SEQ ID NO:86) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLVESGGG VVQPGGSLRL SCAASGFTFS 
               
               
                   
               
               
                   
                     SYGMH   WVRQA PGKGLEWVA   F       IRYDGSNKYY     
               
               
                   
               
               
                   
                     ADSVKG   RFTI SRDNSKNTKY LQMNSLRAED 
               
               
                   
               
               
                   
                 TAVYYCAK   DR       FGYYLDY   WGQ GTLVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of KYK-1.0 (SEQ ID NO:87) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QPVLTQPSSV SVAPGETARI PC   GGDDIETK     
               
               
                   
               
               
                   
                     SV   H   WYQQKPG QAPVLVIY   DD       DDRPS   GIPER 
               
               
                   
               
               
                   
                 FFGSNSGNTA TLSISRVEAG DEADYYC   QVW     
               
               
                   
               
               
                   
                     DDNNDEWV   FG GGTQLTVL 
               
            
           
         
       
     
     The amino acid sequence of a VH Domain of KYK-2.0 (SEQ ID NO:88) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLVESGGG LVKPGGSLRL SCAASGFTFS 
               
               
                   
               
               
                   
                     SYGMH   WVRQA PGKGLEWVA   F       IRYDGSNKYY     
               
               
                   
               
               
                   
                     ADSVKG   RFTI SRDNSKNTLY LQMNSLRAED 
               
               
                   
               
               
                   
                 TAVYYCAKD   R       GLGDGTYFDY    WGQGTTVTVS 
               
               
                   
               
               
                   
                 S 
               
            
           
         
       
     
     The amino acid sequence of a VL Domain of KYK-2.0 (SEQ ID NO:89) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QSALTQPASV SGSPGQSITI SC   SGSSSNIG     
               
               
                   
               
               
                   
                     NNAVN   WYQQL PGKAPKLLIY    YDDLLPS   GVS 
               
               
                   
               
               
                   
                 DRFSGSKSGT SAFLAISGLQ SEDEADYYC   A     
               
               
                   
               
               
                   
                     AWDDSLNGPV    FGGGTKLTVL 
               
            
           
         
       
     
     Other exemplary antibodies that bind to the cell surface of a Natural Killer cell include antibodies: A1, AC2, EPR3678(2), EPR20461, EPR20627 and IMG17B5F11 (which bind CD39); TB01, HNK-1/Leu-7 and NK1 (which bind CD57); FN50 (which binds CD69); 5B5, B-L2, TS82b and C33 (which bind CD82); 3B3, B199.2 and EP7169 (which bind CD161); 17D9 (which binds CLEC1B); 2F9 (which binds KIR2DL1); EPR8825 (which binds KIR2DL2); mAb 33 (which binds KIR2DL4); 11E3, 17B4, EPR4392(2), EPR20261 and EPR 20627 (which bind Lymphocyte Activation Gene 3); A10, C7, CX5, 1D11 and MM0489-10R27 (which bind NKG2D); BMK13 (which binds PRG2); EPR9916 (which binds SLAMF6); etc. Antibodies capable of binding to each of such exemplary non-CD16 cell surface molecule are commercially available from Abcam plc and other sources, and may be readily adapted to the purposes of the present invention. 
     2. Exemplary Anti-CD2 Antibodies 
     In one embodiment, the molecules of the present invention that are capable of mediating the redirected killing of a target cell will bind an effector cell by immunospecifically binding an epitope of CD2 present on the surface of such effector cell. Molecules that specifically bind CD2 include the anti-CD2 antibody “CD2 mAb Lo-CD2a.” 
     The amino acid sequence of the VH Domain of CD2 mAb Lo-CD2a (ATCC Accession No: 11423); SEQ ID NO:90) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLQQSGPE LQRPGASVKL SCKASGYIFT 
               
               
                   
               
               
                   
                     EYYMY   WVKQR PKQGLELVG   R       IDPEDGSIDY     
               
               
                   
               
               
                   
                     VEKFKK   KATL TADTSSNTAY MQLSSLTSED 
               
               
                   
               
               
                   
                 TATYFCAR   GK       FNYRFAY   WGQ GTLVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of CD2 mAb Lo-CD2a (ATCC Accession No: 11423; SEQ ID NO:91) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DVVLTQTPPT LLATIGQSVS ISC   RSSQSLL     
               
               
                   
               
               
                   
                     HSSGNTYLN   W LLQRTGQSPQ PLIY   LVSKLE     
               
               
                   
               
               
                   
                     S   GVPNRFSGS GSGTDFTLKI SGVEAEDLGV 
               
               
                   
               
               
                   
                 YYC   MQFTHYP       YT   FGAGTKLE LK 
               
            
           
         
       
     
     3. Exemplary Anti-CD8 Antibodies 
     In one embodiment, the molecules of the present invention that are capable of mediating the redirected killing of a target cell will bind an effector cell by immunospecifically binding an epitope of CD8 present on the surface of such effector cell. Antibodies that specifically bind CD8 include the anti-CD8 antibodies “OKT8” and “TRX2.” 
     The amino acid sequence of the VH Domain of OKT8 (SEQ ID NO:92) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLLESGPE LLKPGASVKM SCKA   SGYTFT     
               
               
                   
               
               
                   
                     DYNMH   WVKQS HGKSLEWIG   Y       IYPYTGGTGY     
               
               
                   
               
               
                   
                     NQKFKN   KATL TVDSSSSTAY MELRSLTSED 
               
               
                   
               
               
                   
                 SAVYYCARNF RYTYWYFDVW GQGTTVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of OKT8 (SEQ ID NO:93) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIVMTQSPAS LAVSLGQRAT ISCRASESVD 
               
               
                   
               
               
                   
                     SYDNSLMH   WY QQKPGQPPKV LIY   LASNLES     
               
               
                   
               
               
                   
                 GVPARFSGSG SRTDFTLTID PVEADDAATY 
               
               
                   
               
               
                   
                 YC   QQNNEDPY       T   FGGGTKLEI KR 
               
            
           
         
       
     
     The amino acid sequence of the VH Domain of TRX2 (SEQ ID NO:94) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLVESGGG VVQPGRSLRL SCAASGFTFS 
               
               
                   
               
               
                   
                     DFGMN   WVRQA PGKGLEWVA   L       IYYDGSNKFY     
               
               
                   
               
               
                   
                     ADSVKG   RFTI SRDNSKNTLY LQMNSLRAED 
               
               
                   
               
               
                   
                 TAVYYCAK   PH       YDGYYHFFDS    WGQGTLVTVS 
               
               
                   
               
               
                   
                 S 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of TRX2 (SEQ ID NO:95) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   KGSQDIN     
               
               
                   
               
               
                   
                     NYLA   WYQQKP GKAPKLLIY   N       TDILHT   GVPS 
               
               
                   
               
               
                   
                 RFSGSGSGTD FTFTISSLQP EDIATYYC   YQ     
               
               
                   
               
               
                   
                     YNNGYT   FGQG TKVEIK 
               
            
           
         
       
     
     VI. Exemplary Disease Antigens 
     The Disease Antigens of the present invention comprise cell surface antigens that are characteristic of a cancer cell (“Cancer Antigens”) as well as cell surface antigens that are characteristic of a pathogen cell or a cell infected by a pathogen (Pathogen-Associated Antigens”). 
     A. Exemplary Cancer Antigens Arrayed on the Surface of Cancer Cells 
     As used herein, the term “Cancer Antigen” denotes an antigen that is characteristically expressed on the surface of a cancer cell, and that may thus be treated with an Antibody-Based Molecule or an Immunomodulatory Molecule. Examples of Cancer Antigens include, but are not limited to: 19.9 as found in colon cancer, gastric cancer mucins; 4.2; A33 (a colorectal carcinoma antigen; Almqvist, Y. (2006) “ In vitro and in vivo Characterization of  177 Lu - huA 33 : A Radioimmunoconjugate Against Colorectal Cancer ,” Nucl. Med. Biol. 33(8):991-998); ADAM-9 (United States Patent Publication No. 2006/0172350; PCT Publication No. WO 06/084075); AH6 as found in gastric cancer; ALCAM (PCT Publication No. WO 03/093443); APO-1 (malignant human lymphocyte antigen) (Trauth, B. C. et al. (1989) “ Monoclonal Antibody - Mediated Tumor Regression By Induction Of Apoptosis ,” Science 245:301-304); B1 (Egloff, A. M. et al. (2006) “ Cyclin B 1  And Other Cyclins As Tumor Antigens In Immunosurveillance And Immunotherapy Of Cancer ,” Cancer Res. 66(1):6-9); B7-H3 (Collins, M. et al. (2005) “ The B 7  Family Of Immune - Regulatory Ligands ,” Genome Biol. 6:223.1-223.7). Chapoval, A. et al. (2001) “ B 7- H 3 : A Costimulatory Molecule For T Cell Activation and IFN - γ Production ,” Nature Immunol. 2:269-274; Sun, M. et al. (2002) “ Characterization of Mouse and Human B 7- H 3  Genes ,” J. Immunol. 168:6294-6297); BAGE (Bodey, B. (2002) “ Cancer - Testis Antigens: Promising Targets For Antigen Directed Antineoplastic Immunotherapy ,” Expert Opin. Biol. Ther. 2(6):577-584); beta-catenin (Prange W. et al. (2003) “ Beta - Catenin Accumulation In The Progression Of Human Hepatocarcinogenesis Correlates With Loss Of E - Cadherin And Accumulation Of P 53 , But Not With Expression Of Conventional WNT -1  Target Genes ,” J. Pathol. 201(2):250-259); blood group ALe b /Le y  as found in colonic adenocarcinoma; Burkitt&#39;s lymphoma antigen-38.13; C14 as found in colonic adenocarcinoma; CA125 (ovarian carcinoma antigen) (Bast, R. C. Jr. et al. (2005) “ New Tumor Markers: CA 125  And Beyond ,” Int. J. Gynecol. Cancer 15(Suppl 3):274-281; Yu et al. (1991) “ Coexpression Of Different Antigenic Markers On Moieties That Bear CA  125  Determinants ,” Cancer Res. 51(2):468-475); Carboxypeptidase M (United States Patent Publication No. 2006/0166291); CD5 (Calin, G. A. et al. (2006) “ Genomics Of Chronic Lymphocytic Leukemia MicroRNAs As New Players With Clinical Significance ,” Semin. Oncol. 33(2):167-173; CD19 (Ghetie et al. (1994) “ Anti - CD 19  Inhibits The Growth Of Human B - Cell Tumor Lines In Vitro And Of Daudi Cells In SCID Mice By Inducing Cell Cycle Arrest ,” Blood 83:1329-1336; Troussard, X. et al. 1998 Hematol Cell Ther. 40(4):139-48); CD20 (Reff et al. (1994) “ Depletion Of B Cells In Vivo By A Chimeric Mouse Human Monoclonal Antibody To CD 20,” Blood 83:435-445; Thomas, D. A. et al. 2006 Hematol Oncol Clin North Am. 20(5):1125-36); CD22 (Kreitman, R. J. (2006) “ Immunotoxins For Targeted Cancer Therapy ,” AAPS J. 8(3):E532-51); CD23 (Rosati, S. et al. (2005) “ Chronic Lymphocytic Leukaemia: A Review Of The Immuno - Architecture ,” Curr. Top. Microbiol. Immunol. 294:91-107); CD25 (Troussard, X. et al. (1998) “ Hairy Cell Leukemia. What Is New Forty Years After The First Description ?” Hematol. Cell. Ther. 40(4):139-148); CD27 (Bataille, R. (2006) “ The Phenotype Of Normal, Reactive And Malignant Plasma Cells. Identification Of “Many And Multiple Myelomas” And Of New Targets For Myeloma Therapy ,” Haematologica 91(9):1234-1240); CD28 (Bataille, R. (2006) “ The Phenotype Of Normal, Reactive And Malignant Plasma Cells. Identification Of “Many And Multiple Myelomas” And Of New Targets For Myeloma Therapy ,” Haematologica 91(9):1234-1240); CD33 (Sgouros et al. (1993) “ Modeling And Dosimetry Of Monoclonal Antibody M 195 ( Anti - CD 33)  In Acute Myelogenous Leukemia ,” J. Nucl. Med. 34:422-430); CD36 (Ge, Y. (2005) “ CD 36 : A Multiligand Molecule,” Lab Hematol.  11(1):31-7); CD40/CD154 (Messmer, D. et al. (2005) “ CD 154  Gene Therapy For Human B - Cell Malignancies ,” Ann. N. Y. Acad. Sci. 1062:51-60); CD45 (Jurcic, J. G. (2005) “ Immunotherapy For Acute Myeloid Leukemia ,” Curr. Oncol. Rep. 7(5):339-346); CD56 (Bataille, R. (2006) “ The Phenotype Of Normal, Reactive And Malignant Plasma Cells. Identification Of “Many And Multiple Myelomas” And Of New Targets For Myeloma Therapy ,” Haematologica 91(9):1234-1240); CD46 (U.S. Pat. No. 7,148,038; PCT Publication No. WO 03/032814); CD52 (Eketorp, S. S. et al. (2014) “ Alemtuzumab  ( Anti - CD 52  Monoclonal Antibody )  As Single - Agent Therapy In Patients With Relapsed/Refractory Chronic Lymphocytic Leukaemia  ( CLL )- A Single Region Experience On Consecutive Patients ,” Ann Hematol. 93(10):1725-1733; Suresh, T. et al. (2014) “ New Antibody Approaches To Lymphoma Therapy ,” J. Hematol. Oncol. 7:58; Hoelzer, D. (2013) “ Targeted Therapy With Monoclonal Antibodies In Acute Lymphoblastic Leukemia ,” Curr. Opin. Oncol. 25(6):701-706); CD56 (Bataille, R. (2006) “ The Phenotype Of Normal, Reactive And Malignant Plasma Cells. Identification Of “Many And Multiple Myelomas” And Of New Targets For Myeloma Therapy ,” Haematologica 91(9):1234-1240); CD79a/CD79b (Troussard, X. et al. (1998) “ Hairy Cell Leukemia. What Is New Forty Years After The First Description ?” Hematol. Cell. Ther. 40(4): 139-148; Chu, P. G. et al. (2001) “ CD 79 : A Review ,” Appl. Immunohistochem. Mol. Morphol. 9(2):97-106); CD103 (Troussard, X. et al. (1998) “ Hairy Cell Leukemia. What Is New Forty Years After The First Description ?” Hematol. Cell. Ther. 40(4):139-148); CD317 (Kawai, S. et al. (2008) “ Interferon - A Enhances CD 317  Expression And The Antitumor Activity Of Anti - CD 317  Monoclonal Antibody In Renal Cell Carcinoma Xenograft Models ,” Cancer Science 99(12):2461-2466; Wang, W. et al. (2009)  HM 1.24 ( CD 317)  Is A Novel Target Against Lung Cancer For Immunotherapy Using Anti - HM 1.24  Antibody ,” Cancer Immunology, Immunotherapy 58(6):967-976; Wang, W. et al. (2009) “ Chimeric And Humanized Anti - HM 1.24  Antibodies Mediate Antibody - Dependent Cellular Cytotoxicity Against Lung Cancer Cells. Lung Cancer,”  63(1):23-31; Sayeed, A. et al. (2013) “ Aberrant Regulation Of The BST 2 ( Tetherin )  Promoter Enhances Cell Proliferation And Apoptosis Evasion In High Grade Breast Cancer Cells ,” PLoS ONE 8(6)e67191, pp. 1-10); CDK4 (Lee, Y. M. et al. (2006) “ Targeting Cyclins And Cyclin - Dependent Kinases In Cancer: Lessons From Mice, Hopes For Therapeutic Applications In Human ,” Cell Cycle 5(18):2110-2114); CEA (carcinoembryonic antigen; Foon et al. (1995) “ Immune Response To The Carcinoembryonic Antigen In Patients Treated With An Anti - Idiotype Antibody Vaccine ,” J. Clin. Invest. 96(1):334-42); Mathelin, C. (2006) “ Circulating Proteinic Biomarkers And Breast Cancer ,” Gynecol. Obstet. Fertil. 34(7-8):638-646; Tellez-Avila, F. I. et al. (2005) “ The Carcinoembryonic Antigen: Apropos Of An Old Friend ,” Rev. Invest. Clin. 57(6):814-819); CEACAM5/CEACAM6 (Zheng, C. et al. (2011) “ A Novel Anti - CEACAM 5  Monoclonal Antibody, CC 4 , Suppresses Colorectal Tumor Growth and Enhances NK Cells - Mediated Tumor Immunity ,” PLoS One 6(6):e21146, pp. 1-11); CO17-1A (Ragnhammar et al. (1993) “ Effect Of Monoclonal Antibody  17-1 A And GM - CSF In Patients With Advanced Colorectal Carcinoma—Long - Lasting, Complete Remissions Can Be Induced ,” Int. J. Cancer 53:751-758); CO-43 (blood group Le b ); CO-514 (blood group Le a ) as found in adenocarcinoma; CTA-1; CTLA-4 (Peggs, K. S. et al. (2006) “ Principles And Use Of Anti - CTLA 4  Antibody In Human Cancer Immunotherapy ,” Curr. Opin. Immunol. 18(2):206-13); Cytokeratin 8 (PCT Publication No. WO 03/024191); D1.1; D 1 56-22; DR5 (Abdulghani, J. et al. (2010) “ TRAIL Receptor Signaling And Therapeutics ,” Expert Opin. Ther. Targets 14(10): 1091-1108; Andera, L. (2009) “ Signaling Activated By The Death Receptors Of The TNFR Family ,” Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech. Repub. 153(3): 173-180; Carlo-Stella, C. et al. (2007) “ Targeting TRAIL Agonistic Receptors for Cancer Therapy ,” Clin, Cancer 13(8):2313-2317; Chaudhari, B. R. et al. (2006) “ Following the TRAIL to Apoptosis ,” Immunologic Res. 35(3):249-262); E 1  series (blood group B) as found in pancreatic cancer; EGFR (Epidermal Growth Factor Receptor; Adenis, A. et al. (2003) “ Inhibitors Of Epidermal Growth Factor Receptor And Colorectal Cancer ,” Bull. Cancer. 90 Spec No: 5228-5232); Ephrin receptors (and in particular EphA2 (U.S. Pat. No. 7,569,672; PCT Publication No. WO 06/084226); Erb (ErbB1; ErbB3; ErbB4; Zhou, H. et al. (2002) “ Lung Tumorigenesis Associated With Erb - B -2  And Erb - B -3  Overexpression In Human Erb - B -3  Transgenic Mice Is Enhanced By Methylnitrosourea,” Oncogene  21(57):8732-8740; Rimon, E. et al. (2004) “ Gonadotropin Induced Gene Regulation In Human Granulosa Cells Obtained From IVF Patients: Modulation Of Genes Coding For Growth Factors And Their Receptors And Genes Involved In Cancer And Other Diseases ,” Int. J. Oncol. 24(5):1325-1338); GAGE (GAGE-1; GAGE-2; Akcakanat, A. et al. (2006) “ Heterogeneous Expression Of GAGE, NY - ESO -1 , MAGE - A and SSX Proteins In Esophageal Cancer: Implications For Immunotherapy ,” Int. J. Cancer. 118(1): 123-128); GD2/GD3/GM2 (Livingston, P. O. et al. (2005) “ Selection Of GM 2 , Fucosyl GML Globo H And Polysialic Acid As Targets On Small Cell Lung Cancers For Antibody Mediated Immunotherapy ,” Cancer Immunol. Immunother. 54(10):1018-1025); ganglioside GD2 (GD2; Saleh et al. (1993) “ Generation Of A Human Anti - Idiotypic Antibody That Mimics The GD 2  Antigen ,” J. Immunol., 151, 3390-3398); ganglioside GD3 (G D3 ; Shitara et al. (1993) “ A Mouse/Human Chimeric Anti -(Ganglioside GD3)  Antibody With Enhanced Antitumor Activities ,” Cancer Immunol. Immunother. 36:373-380); ganglioside GM2 (G M2 ; Livingston et al. (1994) “ Improved Survival In Stage III Melanoma Patients With GM 2  Antibodies: A Randomized Trial Of Adjuvant Vaccination With GM 2  Ganglioside ,” J. Clin. Oncol. 12:1036-1044); ganglioside GM3 (GM3; Hoon et al. (1993) “ Molecular Cloning Of A Human Monoclonal Antibody Reactive To Ganglioside GM 3  Antigen On Human Cancers ,” Cancer Res. 53:5244-5250); GICA 19-9 (Herlyn et al. (1982) “ Monoclonal Antibody Detection Of A Circulating Tumor Associated Antigen. I. Presence Of Antigen In Sera Of Patients With Colorectal, Gastric, And Pancreatic Carcinoma ,” J. Clin. Immunol. 2:135-140); gp100 (Lotem, M. et al. (2006) “ Presentation Of Tumor Antigens By Dendritic Cells Genetically Modified With Viral And Nonviral Vectors ,” J. Immunother. 29(6):616-27); Gp37 (human leukemia T cell antigen; Bhattacharya-Chatterjee et al. (1988) “ Idiotype Vaccines Against Human T Cell Leukemia. II. Generation And Characterization Of A Monoclonal Idiotype Cascade  ( Ab 1,  Ab 2 , and Ab 3),” J. Immunol. 141:1398-1403); gp75 (melanoma antigen; Vijayasardahl et al. (1990) “ The Melanoma Antigen Gp 75  Is The Human Homologue Of The Mouse B  ( Brown )  Locus Gene Product ,” J. Exp. Med. 171(4):1375-1380); gpA33 (Heath, J. K. et al. (1997) “ The Human A 33  Antigen Is A Transmembrane Glycoprotein And A Novel Member Of The Immunoglobulin  Superfamily,” Proc. Natl. Acad. Sci. (U.S.A.) 94(2):469-474; Ritter, G. et al. (1997) “ Characterization Of Posttranslational Modifications Of Human A 33  Antigen, A Novel Palmitoylated Surface Glycoprotein Of Human Gastrointestinal Epithelium ,” Biochem. Biophys. Res. Commun. 236(3):682-686; Wong, N. A. et al. (2006) “ EpCAM and gpA 33  Are Markers Of Barrett&#39;s Metaplasia ,” J. Clin. Pathol. 59(3):260-263); HER2 antigen (HER2/neu, p185 HER2 ; Pal, S. K. et al. (2006) “ Targeting HER 2  Epitopes ,” Semin. Oncol. 33(4):386-391); HMFG (human milk fat globule antigen; WO1995015171); human papillomavirus-E6/human papillomavirus-E7 (DiMaio, D. et al. (2006) “ Human Papillomaviruses And Cervical Cancer ,” Adv. Virus Res. 66:125-59; HMW-MAA (high molecular weight melanoma antigen; Natali et al. (1987) “ Immunohistochemical Detection Of Antigen In Human Primary And Metastatic Melanomas By The Monoclonal Antibody  140.240  And Its Possible Prognostic Significance ,” Cancer 59:55-63; Mittelman et al. (1990) “ Active Specific Immunotherapy In Patients With Melanoma. A Clinical Trial With Mouse Antiidiotypic Monoclonal Antibodies Elicited With Syngeneic Anti - High - Molecular - Weight - Melanoma - Associated Antigen Monoclonal Antibodies ,” J. Clin. Invest. 86:2136-2144); I antigen (differentiation antigen; Feizi (1985) “ Demonstration By Monoclonal Antibodies That Carbohydrate Structures Of Glycoproteins And Glycolipids Are Onco - Developmental Antigens ,” Nature 314:53-57); IL13Rα2 (PCT Publication No. WO 2008/146911; Brown, C. E. et al. (2013) “ Glioma IL 13 Rα 2  Is Associated With Mesenchymal Signature Gene Expression And Poor Patient Prognosis ,” PLoS One. 18; 8(10):e77769; Barderas, R. et al. (2012) “ High Expression Of IL -13  Receptor A 2  In Colorectal Cancer Is Associated With Invasion, Liver Metastasis, And Poor Prognosis ,” Cancer Res. 72(11):2780-2790; Kasaian, M. T. et al. (2011) “ IL -13  Antibodies Influence IL -13  Clearance In Humans By Modulating Scavenger Activity Of IL -13 R α2,” J. Immunol. 187(1): 561-569; Bozinov, O. et al. (2010) “ Decreasing Expression Of The Interleukin -13  Receptor IL -13 Ralpha 2  In Treated Recurrent Malignant Gliomas ,” Neurol. Med. Chir. (Tokyo) 50(8):617-621; Fujisawa, T. et al. (2009) “ A novel role of interleukin -13  receptor alpha 2  in pancreatic cancer invasion and metastasis ,” Cancer Res. 69(22):8678-8685); Integrin β6 (PCT Publication No. WO 03/087340); JAM-3 (PCT Publication No. WO 06/084078); KID3 (PCT Publication No. WO 05/028498); KID31 (PCT Publication No. WO 06/076584); KS 1/4 pan-carcinoma antigen (Perez et al. (1989) “ Isolation And Characterization Of A cDNA Encoding The Ks 1/4  Epithelial Carcinoma Marker ,” J. Immunol. 142:3662-3667; Möller et al. (1991) “ Bispecific - Monoclonal - Antibody - Directed Lysis Of Ovarian Carcinoma Cells By Activated Human T Lymphocytes ,” Cancer Immunol. Immunother. 33(4):210-216; Ragupathi, G. 2005 Cancer Treat Res. 123:157-80); L6 and L20 (human lung carcinoma antigens; Hellström et al. (1986) “ Monoclonal Mouse Antibodies Raised Against Human Lung Carcinoma ,” Cancer Res. 46:3917-3923); LEA; LUCA-2 (United States Patent Publication No. 2006/0172349; PCT Publication No. WO 06/083852); M1:22:25:8; M18; M39; MAGE (MAGE-1; MAGE-3; (Bodey, B. (2002) “ Cancer - Testis Antigens: Promising Targets For Antigen Directed Antineoplastic Immunotherapy ,” Expert Opin. Biol. Ther. 2(6):577-584); MART (Kounalakis, N. et al. (2005) “ Tumor Cell And Circulating Markers In Melanoma: Diagnosis, Prognosis, And Management ,” Curr. Oncol. Rep. 7(5):377-382; mesothelin (Chang, K. et al. (1996) “ Molecular Cloning Of Mesothelin, A Differentiation Antigen Present On Mesothelium, Mesotheliomas, And Ovarian Cancers ,” Proc. Natl. Acad. Sci. (U.S.A.) 93:136-140); MUC-1 (Mathelin, C. (2006) “ Circulating Proteinic Biomarkers And Breast Cancer ,” Gynecol. Obstet. Fertil. 34(7-8):638-646); MUM-1 (Castelli, C. et al. (2000) “ T - Cell Recognition Of Melanoma - Associated Antigens ,” J. Cell. Physiol. 182(3):323-331); Myl; N-acetylglucosaminyltransferase (Dennis, J. W. (1999) “ Glycoprotein Glycosylation And Cancer Progression ,” Biochim. Biophys. Acta. 6; 1473(1):21-34); neoglycoprotein; NS-10 as found in adenocarcinomas; OFA-1; OFA-2; Oncostatin M (Oncostatin Receptor Beta; U.S. Pat. No. 7,572,896; PCT Publication No. WO 06/084092); p15 (Gil, J. et al. (2006) “ Regulation Of The INK 4 b - ARF - INK 4 a Tumour Suppressor Locus: All For One Or One For All ,” Nat. Rev. Mol. Cell Biol. 7(9):667-677); p97 (melanoma-associated antigen; Estin et al. (1989) “ Transfected Mouse Melanoma Lines That Express Various Levels Of Human Melanoma - Associated Antigen p 97,” J. Natl. Cancer Instit. 81(6):445-454); PEM (polymorphic epithelial mucin; Hilkens et al. (1992) “ Cell Membrane - Associated Mucins And Their Adhesion Modulating Property ,” Trends in Biochem. Sci. 17:359-363); PEMA (polymorphic epithelial mucin antigen); PIPA (U.S. Pat. No. 7,405,061; PCT Publication No. WO 04/043239); PSA (prostate-specific antigen; Henttu et al. (1989) “ cDNA Coding For The Entire Human Prostate Specific Antigen Shows High Homologies To The Human Tissue Kallikrein Genes ,” Biochem. Biophys. Res. Comm. 10(2):903-910; Israeli et al. (1993) “ Molecular Cloning Of A Complementary DNA Encoding A Prostate - Specific Membrane Antigen ,” Cancer Res. 53:227-230; Cracco, C. M. et al. (2005) “ Immune Response In Prostate Cancer ,” Minerva Urol. Nefrol. 57(4):301-311); PSMA (prostate-specific membrane antigen; Ragupathi, G. (2005) “ Antibody Inducing Polyvalent Cancer Vaccines ,” Cancer Treat. Res. 123:157-180); prostatic acid phosphate (Tailor et al. (1990) “ Nucleotide Sequence Of Human Prostatic Acid Phosphatase Determined From A Full - Length cDNA Clone ,” Nucl. Acids Res. 18(16):4928); R24 as found in melanoma; ROR1 (U.S. Pat. No. 5,843,749); sphingolipids; SSEA-1; SSEA-3; SSEA-4; sTn (Holmberg, L. A. (2001) “ Theratope Vaccine  ( STn - KLH ),” Expert Opin. Biol. Ther. 1(5):881-91); T cell receptor derived peptide from a cutaneous T cell lymphoma (see Edelson (1998) “ Cutaneous T - Cell Lymphoma: A Model For Selective Immunotherapy ,” Cancer J. Sci. Am. 4:62-71); T 5 A 7  found in myeloid cells; TAG-72 (Yokota et al. (1992) “ Rapid Tumor Penetration Of A Single - Chain Fv And Comparison With Other Immunoglobulin Forms ,” Cancer Res. 52:3402-3408); TL5 (blood group A); TNF-receptor (TNF-α receptor, TNF-β receptor; TNF-γ receptor (van Horssen, R. et al. (2006) “ TNF - Alpha In Cancer Treatment: Molecular Insights, Antitumor Effects, And Clinical Utility ,” Oncologist 11(4):397-408; Gardnerova, M. et al. (2000) “ The Use Of TNF Family Ligands And Receptors And Agents Which Modify Their Interaction As Therapeutic Agents ,” Curr. Drug Targets 1(4):327-364); TRA-1-85 (blood group H); Transferrin Receptor (U.S. Pat. No. 7,572,895; PCT Publication No. WO 05/121179); 5T4 (TPBG, trophoblast glycoprotein; Boghaert, E. R. et al. (2008) “ The Oncofetal Protein,  5 T 4 , Is A Suitable Target For Antibody - Guided Anti - Cancer Chemotherapy With Calicheamicin ,” Int. J. Oncol. 32(1):221-234; Eisen, T. et al. (2014) “ Naptumomab Estafenatox: Targeted Immunotherapy with a Novel Immunotoxin ,” Curr. Oncol. Rep. 16:370, pp. 1-6); TSTA (tumor-specific transplantation antigen) such as virally-induced tumor antigens including T-antigen DNA tumor viruses and envelope antigens of RNA tumor viruses, oncofetal antigen-alpha-fetoprotein such as CEA of colon, bladder tumor oncofetal antigen (Hellström et al. (1985) “ Monoclonal Antibodies To Cell Surface Antigens Shared By Chemically Induced Mouse Bladder Carcinomas ,” Cancer. Res. 45:2210-2188); VEGF (Pietrantonio, F. et al. (2015) “ Bevacizumab Based Neoadjuvant Chemotherapy For Colorectal Cancer Liver Metastases: Pitfalls And Helpful Tricks In A Review For Clinicians ,” Crit. Rev. Oncol. Hematol. 95(3): 272-281; Grabowski, J. P. (2015) “ Current Management Of Ovarian Cancer ,” Minerva Med. 106(3):151-156; Field, K. M. (2015) “ Bevacizumab And Glioblastoma: Scientific Review, Newly Reported Updates, And Ongoing Controversies ,” Cancer 121(7):997-1007; Suh, D. H. et al. (2015) “ Major Clinical Research Advances In Gynecologic Cancer In  2014,” J. Gynecol. Oncol. 26(2):156-167; Liu, K. J. et al. (2015) “ Bevacizumab In Combination With Anticancer Drugs For Previously Treated Advanced Non - Small Cell Lung Cancer ,” Tumour Biol. 36(3):1323-1327; Di Bartolomeo, M. et al. (2015) “ Bevacizumab Treatment In The Elderly Patient With Metastatic Colorectal Cancer ,” Clin. Interv. Aging 10:127-133); VEGF Receptor (O&#39;Dwyer. P. J. (2006) “ The Present And Future Of Angiogenesis - Directed Treatments Of Colorectal Cancer ,” Oncologist 11(9):992-998); VEP8; VEP9; VIM-D5; and Y hapten, Le y  as found in embryonal carcinoma cells. Additional Cancer Antigens, and molecules (e.g., antibodies) that bind them are disclosed in Table 11. 5T4, B7-H3, CEACAM5/CEACAM6, CD123, DR5, EGFR, an Ephrin receptor, gpA33, HER2/neu, IL13Rα2, ROR1, and VEGF are particularly preferred “Cancer Antigens” of the present invention. 
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Antibody and Antibody-Based Molecules 
               
            
           
           
               
               
               
            
               
                 Antibody Name 
                 Cancer Antigens 
                 Therapeutic Target Application 
               
               
                   
               
               
                 3F8 
                 Gd2 
                 Neuroblastoma 
               
               
                 8H9 
                 B7-H3 
                 Neuroblastoma, Sarcoma,  
               
               
                   
                   
                 Metastatic Brain Cancers 
               
               
                 Abagovomab 
                 CA-125 
                 Ovarian Cancer 
               
               
                 Adecatumumab 
                 Epcam 
                 Prostate and Breast Cancer 
               
               
                 Afutuzumab 
                 CD20 
                 Lymphoma 
               
               
                 Alacizumab 
                 VEGFR2 
                 Cancer 
               
               
                 Altumomab 
                 CEA 
                 Colorectal Cancer 
               
               
                 Amatuximab 
                 Mesothelin 
                 Cancer 
               
               
                 Anatumomab 
                 TAG-72 
                 Non-Small Cell Lung Carcinoma 
               
               
                 Mafenatox 
                   
                   
               
               
                 Anifrolumab 
                 Interferon A/B 
                 Systemic Lupus Erythematosus 
               
               
                   
                 Receptor 
                   
               
               
                 Anrukinzumab 
                 IL-13 
                 Cancer 
               
               
                 Apolizumab 
                 HLA-DR 
                 Hematological Cancers 
               
               
                 Arcitumomab 
                 CEA 
                 Gastrointestinal Cancer 
               
               
                 Atinumab 
                 RTN4 
                 Cancer 
               
               
                 Bectumomab 
                 CD22 
                 Non-Hodgkin&#39;s  
               
               
                   
                   
                 Lymphoma (Detection) 
               
               
                 Belimumab 
                 BAFF 
                 Non-Hodgkin Lymphoma 
               
               
                 Bevacizumab 
                 VEGF-A 
                 Metastatic Cancer, Retinopathy  
               
               
                   
                   
                 of Prematurity 
               
               
                 Bivatuzumab 
                 CD44 V6 
                 Squamous Cell Carcinoma 
               
               
                 Blinatumomab 
                 CD19 
                 Cancer 
               
               
                 Brentuximab 
                 CD30 (TNFRSF8)  
                 Hematologic Cancers 
               
               
                 Cantuzumab 
                 MUC1 
                 Cancers 
               
               
                 Cantuzumab 
                 Mucin Canag 
                 Colorectal Cancer 
               
               
                 Mertansine 
                   
                   
               
               
                 Caplacizumab 
                 VWF 
                 Cancers 
               
               
                 Capromab 
                 Prostatic  
                 Prostate Cancer (Detection) 
               
               
                   
                 Carcinoma Cells 
                   
               
               
                 Carlumab 
                 MCP-1 
                 Oncology/Immune Indications 
               
               
                 Catumaxomab 
                 Epcam, CD3  
                 Ovarian Cancer,  
               
               
                   
                   
                 Malignant Ascites, 
               
               
                   
                   
                 Gastric Cancer 
               
               
                 Cc49 
                 Tag-72 
                 Tumor Detection 
               
               
                 Cetuximab 
                 EGFR 
                 Metastatic Colorectal  
               
               
                   
                   
                 Cancer and Head 
               
               
                   
                   
                 and Neck Cancer 
               
               
                 Ch.14.18 
                 Undetermined 
                 Neuroblastoma 
               
               
                 
                   Citatuzumab 
                 
                 Epcam 
                 Ovarian Cancer and  
               
               
                   
                   
                 other Solid Tumors 
               
               
                 Cixutumumab 
                 IGF-1 Receptor 
                 Solid Tumors 
               
               
                 Clivatuzumab 
                 MUC1 
                 Pancreatic Cancer 
               
               
                 Conatumumab 
                 TRAIL-R2 
                 Cancer 
               
               
                 Dacetuzumab 
                 CD40 
                 Hematologic Cancers 
               
               
                 Dalotuzumab 
                 Insulin-Like  
                 Cancer 
               
               
                   
                 Growth 
                   
               
               
                   
                 Factor I Receptor 
                   
               
               
                 Daratumumab 
                 CD38 
                 Cancer 
               
               
                 Demcizumab 
                 DLL4 
                 Cancer 
               
               
                 Detumomab 
                 B-Lymphoma Cell  
                 Lymphoma 
               
               
                 Drozitumab 
                 DR5 
                 Cancer 
               
               
                 Duligotumab 
                 HER3 
                 Cancer 
               
               
                 Dusigitumab 
                 ILGF2 
                 Cancer 
               
               
                 Ecromeximab 
                 GD3 
                 Ganglioside Malignant  
               
               
                   
                   
                 Melanoma 
               
               
                 Eculizumab 
                 C5 
                 Paroxysmal Nocturnal  
               
               
                   
                   
                 Hemoglobinuria 
               
               
                 Edrecolomab 
                 Epcam 
                 Colorectal Carcinoma 
               
               
                 Elotuzumab 
                 SLAMF7 
                 Multiple Myeloma 
               
               
                 Elsilimomab 
                 IL-6 
                 Cancer 
               
               
                 Enavatuzumab 
                 TWEAK Receptor  
                 Cancer 
               
               
                 Enlimomab 
                 ICAM-1 (CD54)  
                 Cancer 
               
               
                 Enokizumab 
                 IL9 
                 Asthma 
               
               
                 Enoticumab 
                 DLL4 
                 Cancer 
               
               
                 Ensituximab 
                 5AC 
                 Cancer 
               
               
                 Epitumomab 
                 Episialin 
                 Cancer 
               
               
                 Cituxetan 
                   
                   
               
               
                 Epratuzumab 
                 CD22 
                 Cancer, SLE 
               
               
                 Ertumaxomab 
                 HER2/neu, CD3 
                 Breast Cancer 
               
               
                 Etaracizumab 
                 Integrin A v β 3   
                 Melanoma, Prostate  
               
               
                   
                   
                 Cancer, Ovarian 
               
               
                   
                   
                 Cancer 
               
               
                 Faralimomab 
                 Interferon Receptor  
                 Cancer 
               
               
                 Farletuzumab 
                 Folate Receptor 1 
                 Ovarian Cancer 
               
               
                 Fasinumab 
                 HNGF 
                 Cancer 
               
               
                 Fbta05 
                 CD20 
                 Chronic Lymphocytic  
               
               
                   
                   
                 Leukaemia 
               
               
                 Ficlatuzumab 
                 HGF 
                 Cancer 
               
               
                 Figitumumab 
                 IGF-1 Receptor 
                 Adrenocortical  
               
               
                   
                   
                 Carcinoma, Non-Small 
               
               
                   
                   
                 Cell Lung Carcinoma 
               
               
                 Flanvotumab 
                 TYRP1 
                 Melanoma 
               
               
                   
                 (Glycoprotein 75) 
                   
               
               
                 Fontolizumab 
                 IFN-γ 
                 Crohn&#39;s Disease 
               
               
                 Fresolimumab 
                 TGF-B 
                 Idiopathic Pulmonary  
               
               
                   
                   
                 Fibrosis, Focal 
               
               
                   
                   
                 Segmental Glomerulosclerosis,  
               
               
                   
                   
                 Cancer 
               
               
                 Futuximab 
                 EGFR 
                 Cancer 
               
               
                 Galiximab 
                 CD80 
                 B Cell Lymphoma 
               
               
                 Ganitumab 
                 IGF-I 
                 Cancer 
               
               
                 Gemtuzumab 
                 CD33 
                 Acute Myelogenous Leukemia 
               
               
                 Ozogamicin 
                   
                   
               
               
                 Gevokizumab 
                 IL-β 
                 Diabetes 
               
               
                 Girentuximab 
                 Carbonic  
                 Clear Cell Renal Cell Carcinoma 
               
               
                   
                 Anhydrase 
                   
               
               
                   
                 9 (CA-IX) 
                   
               
               
                 Glembatumumab 
                 GPNMB 
                 Melanoma, Breast Cancer 
               
               
                 Vedotin 
                   
                   
               
               
                 Golimumab 
                 TNF-A 
                 Rheumatoid Arthritis,  
               
               
                   
                   
                 Psoriatic Arthritis, 
               
               
                   
                   
                 Ankylosing Spondylitis 
               
               
                 Ibritumomab 
                 CD20 
                 Non-Hodgkin&#39;s Lymphoma 
               
               
                 Tiuxetan 
                   
                   
               
               
                 Icrucumab 
                 VEGFR-1 
                 Cancer 
               
               
                 Igovomab 
                 CA-125 
                 Ovarian Cancer (Diagnosis) 
               
               
                 Imab362 
                 Cldn18.2 
                 Gastrointestinal  
               
               
                   
                   
                 Adenocarcinomas and 
               
               
                   
                   
                 Pancreatic Tumor 
               
               
                 Imgatuzumab 
                 EGFR 
                 Cancer 
               
               
                 Inclacumab 
                 Selectin P 
                 Cancer 
               
               
                 Indatuximab 
                 SDC1 
                 Cancer 
               
               
                 Ravtansine 
                   
                   
               
               
                 Inotuzumab 
                 CD22 
                 Cancer 
               
               
                 Ozogamicin 
                   
                   
               
               
                 Intetumumab 
                 CD51 
                 Solid Tumors (Prostate Cancer, 
               
               
                   
                   
                 Melanoma) 
               
               
                 Ipilimumab 
                 CD152 
                 Melanoma 
               
               
                 Iratumumab 
                 CD30 (TNFRSF8)  
                 Hodgkin&#39;s Lymphoma 
               
               
                 Itolizumab 
                 CD6 
                 Cancer 
               
               
                 Labetuzumab 
                 CEA 
                 Colorectal Cancer 
               
               
                 Lambrolizumab 
                 PDCD1 
                 Antineoplastic Agent 
               
               
                 Lampalizumab 
                 CFD 
                 Cancer 
               
               
                 Lexatumumab 
                 TRAIL-R2 
                 Cancer 
               
               
                 Libivirumab 
                 Hepatitis B Surface 
                 Hepatitis B 
               
               
                   
                 Antigen 
                   
               
               
                 Ligelizumab 
                 IGHE 
                 Cancer 
               
               
                 Lintuzumab 
                 CD33 
                 Cancer 
               
               
                 Lirilumab 
                 KIR2D 
                 Cancer 
               
               
                 Lorvotuzumab 
                 CD56 
                 Cancer 
               
               
                 Lucatumumab 
                 CD40 
                 Multiple Myeloma,  
               
               
                   
                   
                 Non-Hodgkin&#39;s 
               
               
                   
                   
                 Lymphoma, Hodgkin&#39;s  
               
               
                   
                   
                 Lymphoma 
               
               
                 Lumiliximab 
                 CD23 
                 Chronic Lymphocytic Leukemia 
               
               
                 Mapatumumab 
                 TRAIL-R1 
                 Cancer 
               
               
                 Margetuximab 
                 Ch4d5 
                 Cancer 
               
               
                 Matuzumab 
                 EGFR 
                 Colorectal, Lung and  
               
               
                   
                   
                 Stomach Cancer 
               
               
                 Milatuzumab 
                 CD74 
                 Multiple Myeloma and Other 
               
               
                   
                   
                 Hematological Malignancies 
               
               
                 Minretumomab 
                 TAG-72 
                 Cancer 
               
               
                 Mitumomab 
                 GD3 Ganglioside 
                 Small Cell Lung Carcinoma 
               
               
                 Mogamulizumab 
                 CCR4 
                 Cancer 
               
               
                 Morolimumab 
                 Rhesus Factor 
                 Cancer 
               
               
                 Moxetumomab 
                 CD22 
                 Cancer 
               
               
                 Pasudotox 
                   
                   
               
               
                 Nacolomab 
                 C242 Antigen 
                 Colorectal Cancer 
               
               
                 Tafenatox 
                   
                   
               
               
                 Namilumab 
                 C SF2 
                 Cancer 
               
               
                 Naptumomab 
                 5T4 
                 Non-Small Cell Lung Carcinoma,  
               
               
                 Estafenatox 
                   
                 Renal Cell Carcinoma 
               
               
                 Narnatumab 
                 RON 
                 Cancer 
               
               
                 Nebacumab 
                 Endotoxin 
                 Sepsis 
               
               
                 Necitumumab 
                 EGFR 
                 Non-Small Cell Lung Carcinoma 
               
               
                 Nerelimomab 
                 TNF-A 
                 Cancer 
               
               
                 Nesvacumab 
                 Angiopoietin 2  
                 Cancer 
               
               
                 Nimotuzumab 
                 EGFR 
                 Squamous Cell  
               
               
                   
                   
                 Carcinoma, Head and 
               
               
                   
                   
                 Neck Cancer,  
               
               
                   
                   
                 Nasopharyngeal Cancer, 
               
               
                   
                   
                 Glioma 
               
               
                 Nivolumab 
                 PD-1 
                 Cancer 
               
               
                 Nofetumomab 
                 Undetermined 
                 Cancer 
               
               
                 Merpentan 
                   
                   
               
               
                 Ocaratuzumab 
                 CD20 
                 Cancer 
               
               
                 Ofatumumab 
                 CD20 
                 Chronic Lymphocytic Leukemia 
               
               
                 Olaratumab 
                 PDGF-R A  
                 Cancer 
               
               
                 Olokizumab 
                 IL6 
                 Cancer 
               
               
                 Onartuzumab 
                 Human Scatter 
                 Cancer 
               
               
                   
                 Factor Receptor 
                   
               
               
                   
                 Kinase 
                   
               
               
                 Ontuxizumab 
                 TEM1 
                 Cancer 
               
               
                 Oportuzumab 
                 Epcam 
                 Cancer 
               
               
                 Monatox 
                   
                   
               
               
                 Oregovomab 
                 CA-125 
                 Ovarian Cancer 
               
               
                 Orticumab 
                 Oxldl 
                 Cancer 
               
               
                 Otlertuzumab 
                 CD37 
                 Cancer 
               
               
                 Panitumumab 
                 EGFR 
                 Colorectal Cancer 
               
               
                 Pankomab 
                 Tumor Specific 
                 Ovarian Cancer 
               
               
                   
                 Glycosylation of 
                   
               
               
                   
                 MUC1 
                   
               
               
                 Parsatuzumab 
                 EGFL7 
                 Cancer 
               
               
                 Patritumab 
                 HER3 
                 Cancer 
               
               
                 Pembrolizumab 
                 PD-1 
                 Cancer 
               
               
                 Pemtumomab 
                 MUC1 
                 Cancer 
               
               
                 Perakizumab 
                 IL17A 
                 Arthritis 
               
               
                 Pertuzumab 
                 HER2/neu 
                 Cancer 
               
               
                 Pidilizumab 
                 PD-1 
                 Cancer and Infectious Diseases 
               
               
                 Pinatuzumab 
                 CD22 
                 Cancer 
               
               
                 Vedotin 
                   
                   
               
               
                 Pintumomab 
                 Adenocarcinoma 
                 Adenocarcinoma 
               
               
                   
                 Antigen 
                   
               
               
                 Placulumab 
                 Human TNF  
                 Cancer 
               
               
                 Polatuzumab 
                 CD79B 
                 Cancer 
               
               
                 Vedotin 
                   
                   
               
               
                 Pritoxaximab 
                   E. Coli  Shiga  
                 Cancer 
               
               
                   
                 Toxin Type-1 
                   
               
               
                 Pritumumab 
                 Vimentin 
                 Brain Cancer 
               
               
                 Quilizumab 
                 IGHE 
                 Cancer 
               
               
                 Racotumomab 
                 N- 
                 Cancer 
               
               
                   
                 Glycolylneuraminic 
                   
               
               
                   
                 Acid 
                   
               
               
                 Radretumab 
                 Fibronectin Extra 
                 Cancer 
               
               
                   
                 Domain-B 
                   
               
               
                 Ramucirumab 
                 VEGFR2 
                 Solid Tumors 
               
               
                 Rilotumumab 
                 HGF 
                 Solid Tumors 
               
               
                 Rituximab 
                 CD20 
                 Lymphomas, Leukemias, Some 
               
               
                   
                   
                 Autoimmune Disorders 
               
               
                 Robatumumab 
                 IGF-1 Receptor 
                 Cancer 
               
               
                 Roledumab 
                 RHD 
                 Cancer 
               
               
                 Samalizumab 
                 CD200 
                 Cancer 
               
               
                 Satumomab 
                 TAG-72 
                 Cancer 
               
               
                 Pendetide 
                   
                   
               
               
                 Seribantumab 
                 ERBB3 
                 Cancer 
               
               
                 Setoxaximab 
                   E. Coli  Shiga  
                 Cancer 
               
               
                   
                 Toxin Type-1 
                   
               
               
                 Sgn-CD19a 
                 CD19 
                 Acute Lymphoblastic  
               
               
                   
                   
                 Leukemia and B 
               
               
                   
                   
                 Cell Non-Hodgkin Lymphoma 
               
               
                 Sgn-CD33 a 
                 CD33 
                 Acute Myeloid Leukemia 
               
               
                 Sibrotuzumab 
                 FAP 
                 Cancer 
               
               
                 Siltuximab 
                 IL-6 
                 Cancer 
               
               
                 Solitomab 
                 Epcam 
                 Cancer 
               
               
                 Sontuzumab 
                 Episialin 
                 Cancer 
               
               
                 Tab alumab 
                 BAFF 
                 B Cell Cancers 
               
               
                 Tacatuzumab 
                 Alpha-Fetoprotein 
                 Cancer 
               
               
                 Tetraxetan 
                   
                   
               
               
                 Taplitumomab 
                 CD19 
                 Cancer 
               
               
                 Paptox 
                   
                   
               
               
                 Telimomab 
                 Undetermined 
                 Cancer 
               
               
                 Tenatumomab 
                 Tenascin C 
                 Cancer 
               
               
                 Teneliximab 
                 CD40 
                 Cancer 
               
               
                 Teprotumumab 
                 CD221 
                 Hematologic Tumors 
               
               
                 Ticilimumab 
                 CTLA-4 
                 Cancer 
               
               
                 Tigatuzumab 
                 TRAIL-R2 
                 Cancer 
               
               
                 Tnx-650 
                 I1-13 
                 Hodgkin&#39;s Lymphoma 
               
               
                 Tositumomab 
                 CD20 
                 Follicular Lymphoma 
               
               
                 Tovetumab 
                 CD140a 
                 Cancer 
               
               
                 Trastuzumab 
                 HER2/neu 
                 Breast Cancer 
               
               
                 Trbs07 
                 Gd2 
                 Melanoma 
               
               
                 Tremelimumab 
                 CTLA-4 
                 Cancer 
               
               
                 Tucotuzumab 
                 Epcam 
                 Cancer 
               
               
                 Celmoleukin 
                   
                   
               
               
                 Ublituximab 
                 MS4A1 
                 Cancer 
               
               
                 Urelumab 
                 4-1BB 
                 Cancer 
               
               
                 Vantictumab 
                 Frizzled Receptor 
                 Cancer 
               
               
                 Vapaliximab 
                 AOC3 (VAP-1)  
                 Cancer 
               
               
                 Vatelizumab 
                 ITGA2 
                 Cancer 
               
               
                 Veltuzumab 
                 CD20 
                 Non-Hodgkin&#39;s Lymphoma 
               
               
                 Vesencumab 
                 NRP1 
                 Cancer 
               
               
                 Volociximab 
                 Integrin A5β1 
                 Solid Tumors 
               
               
                 Vorsetuzumab 
                 CD70 
                 Cancer 
               
               
                 Votumumab 
                 Tumor Antigen 
                 Colorectal Tumors 
               
               
                   
                 CTAA16.88 
                   
               
               
                 Zalutumumab 
                 EGFR 
                 Squamous Cell Carcinoma  
               
               
                   
                   
                 of The Head And Neck 
               
               
                 Zatuximab 
                 HER1 
                 Cancer 
               
               
                 Ziralimumab 
                 CD147 
                 Cancer 
               
               
                   
               
            
           
         
       
     
     Exemplary antibodies, whose VH and VL Domains may be used to construct the binding molecules of the present invention that are capable of binding a Cancer Antigen arrayed on the surface of a cancer cell and mediating the redirected killing of such cancer cells are listed in Table 11, additional antibodies that may be used to construct molecules capable of binding a Cancer Antigen arrayed on the surface of a cancer cell and mediating the redirected killing of such cancer cells are provided below. 
     1. Exemplary Anti-B7-H3 Antibodies 
     B7-H3 is a Cancer Antigen that is over-expressed on a wide variety of solid tumor types and is a member of the B7 family of molecules that are involved in immune regulation (see, U.S. Pat. No. 8,802,091; US 2014/0328750; US 2013/0149236; Loo, D. et al. (2012) “ Development Of An Fc - Enhanced Anti - B 7- H 3 Monoclonal Antibody With Potent Antitumor Activity ,” Clin. Cancer Res. 18(14):3834-3845). In particular, several independent studies have shown that human malignant cancer cells (e.g., cancer cells of neuroblastomas and gastric, ovarian and non-small cell lung cancers) exhibit a marked increase in expression of B7-H3 protein and that this increased expression was associated with increased disease severity (Zang, X. et al. (2007) “ The B 7  Family And Cancer Therapy: Costimulation And Coinhibition ,” Clin. Cancer Res. 13:5271-5279), suggesting that B7-H3 is exploited by tumors as an immune evasion pathway (Hofmeyer, K. et al. (2008) “ The Contrasting Role Of B 7- H 3,” Proc. Natl. Acad. Sci. (U.S.A.) 105(30):10277-10278). 
     B7-H3 has also been found to co-stimulate CD4+ and CD8+ T-cell proliferation. B7-H3 also stimulates IFN-γ production and CD8+ lytic activity (Chapoval, A. et al. (2001) “ B 7- H 3 : A Costimulatory Molecule For T Cell Activation and IFN - γ Production ,” Nature Immunol. 2:269-274; Sharpe, A. H. et al. (2002) “ The B 7- CD 28  Superfamily ,” Nature Rev. Immunol. 2:116-126). However, the protein also possibly acts through NFAT (nuclear factor for activated T cells), NF-κB (nuclear factor kappa B), and AP-1 (Activator Protein-1) factors to inhibit T-cell activation (Yi. K. H. et al. (2009) “Fine  Tuning The Immune Response Through B 7- H 3  And B 7- H 4,” Immunol. Rev. 229:145-151). B7-H3 is also believed to inhibit Th1, Th2, or Th17 in vivo (Prasad, D. V. et al. (2004) “ Murine B 7- H 3  Is A Negative Regulator Of T Cells ,” J. Immunol. 173:2500-2506; Fukushima, A. et al. (2007) “B7-H3 Regulates The Development Of Experimental Allergic Conjunctivitis In Mice,” Immunol. Lett. 113:52-57; Yi. K. H. et al. (2009) “ Fine Tuning The Immune Response Through B 7- H 3  And B 7- H 4,” Immunol. Rev. 229:145-151). 
     Preferred B7-H3-binding molecules possess the VL and/or VH Domains, of humanized anti-human B7-H3 monoclonal antibody “B7-H3 mAb-B,” “B7-H3 mAb-C,” “B7-H3 mAb-D,” and more preferably possess 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of such anti-B7-H3 monoclonal antibodies. 
     Upon humanization, antibody B7-H3 mAb-B yielded two variant VH Domains, B7-H3 mAb-B VIII and B7-H3 mAb-B VH2; and two variant VL Domains B7-113 mAb-B VL1 and B7-H3 mAb-B VL2, which may be used in any combination of VH/VL Domains to yield a functional B7-H3 Binding Domain. 
     The amino acid sequence of the VH Domain of B7-H3 mAb-B VIII (SEQ ID NO:96) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLVQSGAE VKKPGASVKV SCKASGYTFT 
               
               
                   
               
               
                   
                     SYWMQ   WVRQA PGQGLEWMG   T       IYPGDGDTRY     
               
               
                   
               
               
                   
                     TQKFKG   RVTI TADKSTSTAY MELSSLRSED 
               
               
                   
               
               
                   
                 TAVYYCAR   RG       IPRLWYFDV   W GQGTTVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VH Domain of B7-H3 mAb-B VH2 (SEQ ID NO:97) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLVQSGAE VKKPGASVKV SCKASGYTFT 
               
               
                   
               
               
                   
                     SYWMQ   WVRQA PGQGLEWMG   T       IYPGGGDTRY     
               
               
                   
               
               
                   
                     TQKFQG   RVTI TADKSTSTAY MELSSLRSED 
               
               
                   
               
               
                   
                 TAVYYCAR   RG       IPRLWYFDV   W GQGTTVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of B7-H3 mAb-B VL1 (SEQ ID NO:98) is shown below (CDR L  residues are shown underlined). 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   RASQDIS     
               
               
                   
               
               
                   
                     NYLN   WYQQKP GKAPKLLIY   Y       TSRLHS   GVPS 
               
               
                   
               
               
                   
                 RFSGSGSGTD FTLTISSLQP EDIATYYC   QQ     
               
               
                   
               
               
                   
                     GNTLPPT   FGG GTKLEIK 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of B7-H3 mAb-B VL2 (SEQ ID NO:99) is shown below (CDR L  residues are shown underlined). 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   RASQSIS     
               
               
                   
               
               
                   
                     SYLN   WYQQKP GKAPKLLIY   Y       TSRLQS   GVPS 
               
               
                   
               
               
                   
                 RFSGSGSGTD FTLTISSLQP EDIATYYC   QQ     
               
               
                   
               
               
                   
                     GNTLPPT   FGG GTKLEIK 
               
            
           
         
       
     
     The amino acid sequence of the VH Domain of humanized B7-H3 mAb-C (SEQ ID NO:100) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLVESGGG LVKPGGSLRL SCAASGFTFS 
               
               
                   
               
               
                   
                     SYGMS   WVRQA PGKGLEWVA   T       INSGGSNTYY     
               
               
                   
               
               
                   
                     PDSLKG   RFTI SRDNAKNSLY LQMNSLRAED 
               
               
                   
               
               
                   
                 TAVYYCAR   HD       GGAMDY   WGQG TTVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of humanized B7-H3 mAb-C (SEQ ID NO:101) is shown below (CDR L  residues are shown underlined). 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   RASESIY     
               
               
                   
               
               
                   
                     SYLA   WYQQKP GKAPKLLVY   N       TKTLPE   GVPS 
               
               
                   
               
               
                   
                 RFSGSGSGTD FTLTISSLQP EDFATYYC   QH     
               
               
                   
               
               
                   
                     HYGTPPWT   FG QGTRLEIK 
               
            
           
         
       
     
     The amino acid sequence of the VH Domain of B7-H3 mAb-D (SEQ ID NO:102) is shown below (CDR H  residues are shown underlined). 
     
       
         
           
               
               
            
               
                   
                 EVQLVESGGG LVQPGGSLRL SCAASGFTFS 
               
               
                   
               
               
                   
                     SFGMH   WVRQA PGKGLEWVA Y   ISSGSGTIYY   
               
               
                   
               
               
                   
                   ADTVKG RFTI SRDNAKNSLY LQMNSLRAED 
               
               
                   
               
               
                   
                 TAVYYCAR   HG       YRYEGFDY   WG QGTTVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of B7-H3 mAb-D (SEQ ID NO:103) is shown below (CDR L  residues are shown underlined). 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSF LSASVGDRVT ITC   KASQNVD     
               
               
                   
               
               
                   
                     TNVA   WYQQKP GKAPKALIY   S       ASYRYS   GVPS 
               
               
                   
               
               
                   
                 RFSGSGSGTD FTLTISSLQP EDFAEYFC   QQ     
               
               
                   
               
               
                   
                     YNNYPFT   FGQ GTKLEIK 
               
            
           
         
       
     
     Particularly preferred, are B7-H3-binding molecules which possess a humanized VH and/or VL Domain including but not limited to “Enoblituzumab” (also known as MGA271; CAS Reg No. 1353485-38-7). Enoblituzumab is an Fc-optimized monoclonal antibody that binds to HER2/neu and mediates enhanced ADCC activity. The amino acid sequences of the complete Heavy and Light Chains of Enoblituzumab are known in the art (see., e.g., WHO Drug Information, 2017, Recommended INN: List 77, 31(1):49). 
     The amino acid sequence of the VH Domain of Enoblituzumab (SEQ ID NO:104) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLVESGGG LVQPGGSLRL SCAASGFTFS 
               
               
                   
               
               
                   
                     SFGMH   WVRQA PGKGLEWVA   Y       ISSDSSAIYY     
               
               
                   
               
               
                   
                     ADTVKG   RFTI SRDNAKNSLY LQMNSLRDED 
               
               
                   
               
               
                   
                 TAVYYCGR   GR       ENIYYGSRLD Y   WGQGTTVTV 
               
               
                   
               
               
                   
                 SS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of Enoblituzumab (SEQ ID NO:105) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQLTQSPSF LSASVGDRVT ITC   KASQNVD     
               
               
                   
               
               
                   
                     TNVA   WYQQKP GKAPKALIY   S       ASYRYS   GVPS 
               
               
                   
               
               
                   
                 RFSGSGSGTD FTLTISSLQP EDFATYYC   QQ     
               
               
                   
               
               
                   
                     YNNYPFT   FGQ GTKLEIK 
               
            
           
         
       
     
     In addition to the above-identified preferred anti-B7-H3 Binding Molecules, the invention contemplates the use of any of the following anti-B7-H3 Binding Molecules: LUCA1; BLA8; PA20; or SKN2 (see, U.S. Pat. Nos. 7,527,969; 8,779,098 and PCT Patent Publication WO 2004/001381); M30; cM30; M30-H1-L1; M30-H1-L2; M30-H1-L3; M30-H1-L4; M30-H1-L5; M30-H1-L6; M30-H1-L7; M30-H4-L1; M30-H4-L2; M30-H4-L3; and M30-H4-L4 (see, US Patent Publication 2013/0078234 and PCT Patent Publication WO 2012/147713); and 8119 (see U.S. Pat. Nos. 7,666,424; 7,737,258; 7,740,845; 8,148,154; 8,414,892; 8,501,471; 9,062,110; US Patent Publication 2010/0143245 and PCT Patent Publication WO 2008/116219). 
     The present invention specifically includes and encompasses CD16 x B7-H3 Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of any of B7-H3 mAb-B, B7-H3 mAb-B VH1, B7-H3 mAb-B VH2, B7-H3 mAb-B VL1, B7-H3 mAb-B VL2, B7-H3 mAb-C, B7-H3 mAb-D, or Enoblituzumab, or any of the other anti-B7-H3 antibodies provided herein; and more preferably possess 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of such anti-B7-H3 monoclonal antibodies. 
     2. Exemplary Anti-CEACAM5 and Anti-CEACAM6 Antibodies 
     Carcinoembryonic Antigen-Related Cell Adhesion Molecules 5 (CEACAM5) and 6 (CEACAM6) have been found to be associated with various types of cancers including medullary thyroid cancer, colorectal cancer, pancreatic cancer, hepatocellular carcinoma, gastric cancer, lung cancer, head and neck cancers, urinary bladder cancer, prostate cancer, uterine cancer, endometrial cancer, breast cancer, hematopoietic cancer, leukemia and ovarian cancer (PCT Pubmication No. WO 2011/034660), and particularly colorectal, gastrointestinal, pancreatic, non-small cell lung cancer (NSCL), breast, thyroid, stomach, ovarian and uterine carcinomas (Zheng, C. et al. (2011) “ A Novel Anti - CEACAM 5  Monoclonal Antibody, CC 4 , Suppresses Colorectal Tumor Growth and Enhances NK Cells - Mediated Tumor Immunity ,” PLoS One 6(6):e21146, pp. 1-11). 
     CEACAM5 has been found to be overexpressed in 90% of gastrointestinal, colorectal and pancreatic cancers, 70% of non-small cell lung cancer cells and 50% of breast cancers (Thompson, J. A. et al. (1991) “ Carcinoembryonic Antigen Gene Family: Molecular Biology And Clinical Perspectives ,” J. Clin. Lab. Anal. 5:344-366). Overexpressed carcinoembryonic antigen-related cellular adhesion molecule 6 (CEACAM6) plays important roles in the invasion and metastasis of a variety of human cancers, including medullary thyroid cancer, colorectal cancer, pancreatic cancer, hepatocellular carcinoma, gastric cancer, lung cancer, head and neck cancers, urinary bladder cancer, prostate cancer, uterine cancer, endometrial cancer, breast cancer, hematopoietic cancer, leukemia and ovarian cancer (PCT Pubmication No. WO 2011/034660; Deng, X. et al. (2014) “ Expression Profiling Of CEACAM 6  Associated With The Tumorigenesis And Progression In Gastric Adenocarcinoma ,” Genet. Mol. Res. 13(3):7686-7697; Cameron, S. et al. (2012) “ Focal Overexpression Of CEACAM 6  Contributes To Enhanced Tumourigenesis In Head And Neck Cancer Via Suppression Of Apoptosis ,” Mol. Cancer 11:74, pp. 1-11; Chapin, C. et al. (2012) “ Distribution And Surfactant Association Of Carcinoembryonic Cell Adhesion Molecule  6  In Human Lung ,” Amer. J. Physiol. Lung Cell. Mol. Physiol. 302(2):L216-L25; Riley, C. J. et al. (2009) “ Design And Activity Of A Murine And Humanized Anti - CEACAM 6  Single - Chain Variable Fragment In The Treatment Of Pancreatic Cancer ,” Cancer Res. 69(5): 1933-1940; Lewis-Wambi, J. S. et al. (2008) “ Overexpression Of CEACAM 6  Promotes Migration And Invasion Of Oestrogen - Deprived Breast Cancer Cells ,” Eur. J. Cancer 44(12):1770-1779; Blumenthal, R. D. et al. (2007) “ Expression Patterns Of CEACAM 5  And CEACAM 6  In Primary And Metastatic Cancers ,” BMC Cancer. 7:2, pp. 1-15). Antibodies that immunospecifically bind CEACAM5 and CEACAM6 are commercially available (Santa Cruz Biotechnology, Inc., Novus Biologicals LLC; Abnova Corporation). 
     The amino acid sequence of the VH Domain of the humanized anti-CEACAM5/ANTI-CEACAM6 antibody 16C3 (EP 2585476) (SEQ ID NO:106) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLQQSGPE VVRPGVSVKI SCKGS   GYTFT     
               
               
                   
               
               
                   
                     DYAMH   WVKQS HAKSLEWIG   L       ISTYSGDTKY     
               
               
                   
               
               
                   
                     NQNFKG   KATM TVDKSASTAY MELSSLRSED 
               
               
                   
               
               
                   
                 TAVYYCAR   GD       YSGSRYWFAY    WGQGTLVTVS 
               
               
                   
               
               
                   
                 S 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of the humanized anti-CEACAM5/ANTI-CEACAM6 antibody 16C3 (EP 2585476) (SEQ ID NO:107) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   GASENIY     
               
               
                   
               
               
                   
                     GALN   WYQRKP GKSPKLLIW   G       ASNLAD   GMPS 
               
               
                   
               
               
                   
                 RFSGSGSGRQ YTLTISSLQP EDVATYY   CQN     
               
               
                   
               
               
                   
                     VLSSPYT   FGG GTKLEIK 
               
            
           
         
       
     
     The amino acid sequence of the VH Domain of the humanized anti-CEACAM5/CEACAM6 antibody hMN15 (WO 2011/034660) (SEQ ID NO:108) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLVESGGG VVQPGRSLRL SCS   SSGFALT     
               
               
                   
               
               
                   
                     DYYMS   WVRQA PGKGLEWLG   F       IANKANGHTT     
               
               
                   
               
               
                   
                     DYSPSVKG   RF TISRDNSKNT LFLQMDSLRP 
               
               
                   
               
               
                   
                 EDTGVYFCAR    DMGIRWNFDV    WGQGTPVTVS 
               
               
                   
               
               
                   
                 S 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of the humanized anti-CEACAM5/CEACAM6 antibody hMN15 (WO 2011/034660) (SEQ ID NO:109) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQLTQSPSS LSASVGDRVT MTC   SASSRVS     
               
               
                   
               
               
                   
                     YIH   WYQQKPG KAPKRWIY   GT       STLAS   GVPAR 
               
               
                   
               
               
                   
                 FSGSGSGTDF TFTISSLQPE DIATYYC   QQW     
               
               
                   
               
               
                   
                     SYNPPT   FGQG TKVEIKR 
               
            
           
         
       
     
     The present invention specifically includes and encompasses CD16 x CEACAM5/CEACAM6 Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-CEACAM5/CEACAM6 monoclonal antibodies 16C3 or hMN15. 
     3. Exemplary Anti-EGRF Antibodies 
     Epidermal Growth Factor Receptor (EGFR) is a Cancer Antigen of certain metastatic colorectal cancer, metastatic non-small cell lung cancer and head and neck cancer. Exemplary antibodies that bind human EGRF are “Cetuximab” and “Panitumumab.” Cetuximab is a recombinant human-mouse chimeric epidermal growth factor receptor (EGFR) IgG1 monoclonal antibody (Govindan R. (2004) “ Cetuximab In Advanced Non - Small Cell Lung Cancer ,” Clin. Cancer Res. 10(12 Pt 2):42415-4244s; Bou-Assaly, W. et al. (2010) “ Cetuximab  ( Erbitux ),” Am. J. Neuroradiol. 31(4):626-627). Panitumumab (Vectibix®, Amgen) is a fully humanized epidermal growth factor receptor (EGFR) IgG2 monoclonal antibody (Foon, K. A. et al. (2004) “ Preclinical And Clinical Evaluations Of ABX - EGF, A Fully Human Anti - Epidermal Growth Factor Receptor Antibody ,” Int. J. Radiat. Oncol. Biol. Phys. 58(3):984-990; Yazdi, M. H. et al. (2015) “ A Comprehensive Review of Clinical Trials on EGFR Inhibitors Such as Cetuximab and Panitumumab as Monotherapy and in Combination for Treatment of Metastatic Colorectal Cancer ,” Avicenna J. Med. Biotechnol. 7(4):134-144). 
     The amino acid sequence of the VH Domain of the chimeric anti-EGFR antibody Cetuximab (SEQ ID NO:110) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLKQSGPG LVQPSQSLSI TCTVS   GFSLT     
               
               
                   
               
               
                   
                     NYGVH   WVRQS PGKGLEWLG   V       IWSGGNTDYN     
               
               
                   
               
               
                   
                     TPFTS   RLSIN KDNSKSQVFF KMNSLQSNDT 
               
               
                   
               
               
                   
                 AIYYCAR   ALT       YYDYEFAY   WG QGTLVTVSA 
               
               
                   
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of the chimeric anti-EGFR antibody Cetuximab (SEQ ID NO:111) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DILLTQSPVI LSVSPGERVS FSC   RASQSIG TNIH   WYQQRT 
               
               
                   
                   
               
               
                   
                 NGSPRLLIK   Y       ASESIS   GIPS RFSGSGSGTD FTLSINSVES 
               
               
                   
                   
               
               
                   
                 EDIADYYC   QQ NNNWPTT   FGA GTKLELKR 
               
            
           
         
       
     
     The amino acid sequence of the VH Domain of Panitumumab (SEQ ID NO:112) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLQESGPG LVKPSETLSL TCTVS   GGSVS       SGDYY   WTWIR 
               
               
                   
                   
               
               
                   
                 QSPGKGLEWI G   HIYYSGNTN       YNPSLKS   RLT ISIDTSKTQF 
               
               
                   
                   
               
               
                   
                 SLKLSSVTAA DTAIYYCVR   D       RVTGAFDI   WG QGTMVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of Panitumumab (SEQ ID NO:113) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   QASQDIS       NYLN   WYQQKP 
               
               
                   
                   
               
               
                   
                 GKAPKLLIY   D       ASNLET   GVPS RFSGSGSGTD FTFTISSLQP 
               
               
                   
                   
               
               
                   
                 EDIATYFC   QH       FDHLPLA   FGG GTKVEIKR 
               
            
           
         
       
     
     The present invention specifically includes and encompasses CD16 x EGFR Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-EGFR monoclonal antibodies Cetuximab or Panitumumab. 
     4. Exemplary Anti-EphA2 Antibodies 
     The receptor tyrosine kinase, Ephrin type-A receptor 2 (EphA2) is normally expressed at sites of cell-to-cell contact in adult epithelial tissues, however, recent studies have shown that it is also overexpressed in various types of epithelial carcinomas, with the greatest level of EphA2 expression observed in metastatic lesions. High expression levels of EphA2 have been found in a wide range of cancers and in numerous cancer cell lines, including prostate cancer, breast cancer, non-small cell lung cancer and melanoma (Xu, J. et al. (2014) “ High EphA 2  Protein Expression In Renal Cell Carcinoma Is Associated With A Poor Disease Outcome ,” Oncol. Lett. August 2014; 8(2): 687-692; Miao, B. et al. (2014) “ EphA 2  is a Mediator of Vemurafenib Resistance and a Novel Therapeutic Target in Melanoma ,” Cancer Discov. pii: CD-14-0295). EphA2 does not appear to be merely a marker for cancer, but rather appears to be persistently overexpressed and functionally changed in numerous human cancers (Chen, P. et al. (2014) “ EphA 2  Enhances The Proliferation And Invasion Ability Of LnCap Prostate Cancer Cells ,” Oncol. Lett. 8(1):41-46). Exemplary antibodies that bind human EphA2 are “EphA2 mAb 1,” “EphA2 mAb 2” and “EphA2 mAb 3.” 
     The amino acid sequence of the VH Domain of EphA2 mAb 1 (SEQ ID NO:114) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLKESGPG LVAPSQSLSI TCTVSGFSLS    RYSVH   WVRQP 
               
               
                   
                   
               
               
                   
                 PGKGLEWLG   M       IWGGGSTDYN   SALKSRLSIS KDNSKSQVFL 
               
               
                   
                   
               
               
                   
                 KMNSLQTDDT AMYYCAR   KHG       NYYTMDY   WGQ GTSVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of EphA2 mAb 1 (SEQ ID NO:115) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQTTSS LSASLGDRIT ISC   RASQDIS NYLN   WYQQKP 
               
               
                   
                   
               
               
                   
                 DGTVKLLIY   Y       TSRLHS   GVPS RFSGSGSGTD YSLTISNLEQ 
               
               
                   
                   
               
               
                   
                 EDIATYFC   QQ GYTLYT   FGGG TKLEIK 
               
            
           
         
       
     
     The amino acid sequence of the VH Domain of EphA2 mAb 2 (SEQ ID NO:116) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QIQLVQSGPE LKKPGETVKI SCKASGFTFT    NYGMN   WVKQA 
               
               
                   
                   
               
               
                   
                 PGKGLKWMG   W       INTYIGEPTY ADDFKG   RFVF SLETSASTAY 
               
               
                   
                   
               
               
                   
                 LQINNLKNED MATYFCAR   EL       GPYYFDY   WGQ GTTLTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of EphA2 mAb 2 (SEQ ID NO:117) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DVVMTQTPLS LPVSLGDQAS ISC   RSSQSLV HSSGNTYLH   W 
               
               
                   
                   
               
               
                   
                 YLQKPGQSPK LLIY   KVSNRF S   GVPDRFSGS GSGTDFTLKI 
               
               
                   
                   
               
               
                   
                 SRVEAEDLGV YFC   SQSTHVP       T   FGSGTKLEI K 
               
            
           
         
       
     
     The amino acid sequence of the VH Domain of EphA2 mAb 3 (SEQ ID NO:118) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLVESGGG SVKPGGSLKL SCAASGFTFT    DHYMY   WVRQT 
               
               
                   
                   
               
               
                   
                 PEKRLEWVA   T       ISDGGSFTSY PDSVKG   RFTI SRDIAKNNLY 
               
               
                   
                   
               
               
                   
                 LQMSSLKSED TAMYYCTR   DE       SDRPFPY   WGQ GTLVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of EphA2 mAb 3 (SEQ ID NO:119) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIVLTQSHRS MSTSVGDRVN ITC   KASQDVT TAVA   WYQQKP 
               
               
                   
                   
               
               
                   
                 GQSPKLLIF   W       ASTRHA   GVPD RFTGSGSGTD FTLTISSVQA 
               
               
                   
                   
               
               
                   
                 GDLALYYC   QQ HYSTPYT   FGG GTKLEIK 
               
            
           
         
       
     
     The present invention specifically includes and encompasses CD16 x EphA2 Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L 5 of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of anti-EphA2 monoclonal antibodies EphA2 mAb 1, EphA2 mAb 2 and EphA2 mAb 3. 
     5. Exemplary Anti-gpA33 Antibodies 
     The 43 kD transmembrane glycoprotein A33 (gpA33) is expressed in &gt;95% of all colorectal carcinomas (Heath, J. K. et al. (1997) “ The Human A 33  Antigen Is A Transmembrane Glycoprotein And A Novel Member Of The Immunoglobulin Superfamily ,” Proc. Natl. Acad. Sci. (U.S.A.) 94(2):469-474; Ritter, G. et al. (1997) “ Characterization Of Posttranslational Modifications Of Human A 33  Antigen, A Novel Palmitoylated Surface Glycoprotein Of Human Gastrointestinal Epithelium ,” Biochem. Biophys. Res. Commun. 236(3):682-686; Wong, N. A. et al. (2006) “ EpCAM and gpA 33  Are Markers Of Barrett&#39;s Metaplasia ,” J. Clin. Pathol. 59(3):260-263). An exemplary antibody that binds to human gpA33 is “gpA33 mAb 1.” 
     The amino acid sequence of the VH Domain of gpA33 mAb 1 (SEQ ID NO:120) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLVQSGAE VKKPGASVKV SCKASGYTFT    GSWMN   WVRQA 
               
               
                   
                   
               
               
                   
                 PGQGLEWIG   R       IYPGDGETNY NGKFKD   RVTI TADKSTSTAY 
               
               
                   
                   
               
               
                   
                 MELSSLRSED TAVYYCAR   IY       GNNVYFDV   WG QGTTVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of gpA33 mAb 1 (SEQ ID NO:121) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQLTQSPSF LSASVGDRVT ITC   SARSSIS FMY   WYQQKPG 
               
               
                   
                   
               
               
                   
                 KAPKLLIY   DT       SNLAS   GVPSR FSGSGSGTEF TLTISSLEAE 
               
               
                   
                   
               
               
                   
                 DAATYYC   QQW SSYPLT   FGQG TKLEIK 
               
            
           
         
       
     
     The present invention specifically includes and encompasses CD16 x gpA33 Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of anti-gpA33 monoclonal antibodies gpA33 mAb 1, or of any of the anti-gpA33 monoclonal antibodies provided in WO 2015/026894. 
     6. Exemplary Anti HER2/Neu Antibodies 
     HER2/neu is a 185 kDa receptor protein that was originally identified as the product of the transforming gene from neuroblastomas of chemically treated rats. HER2/neu has been extensively investigated because of its role in several human carcinomas and in mammalian development (Hynes et al. (1994) Biochim. Biophys. Acta 1198:165-184; Dougall et al. (1994) Oncogene 9:2109-2123; Lee et al. (1995) Nature 378:394-398). Exemplary antibodies that bind human HER2/neu include “Margetuximab,” “Trastuzumab” and “Pertuzumab.” Margetuximab (also known as MGAH22; CAS Reg No. 1350624-75-7) is an Fc-optimized monoclonal antibody that binds to HER2/neu and mediates enhanced ADCC activity. Trastuzumab (also known as rhuMAB4D5, and marketed as H ERCEPTIN ®; CAS Reg No 180288-69-1; see, U.S. Pat. No. 5,821,337) is the humanized version of antibody 4D5, having IgG1/kappa constant regions. Pertuzumab (also known as rhuMAB2C4, and marketed as P ERJETA ™; CAS Reg No 380610-27-5; see for example, WO2001/000245) is a humanized version of antibody 2C4 having IgG1/kappa constant regions. 
     The present invention specifically includes and encompasses CD16 x HER2/neu Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-HER2/neu monoclonal antibodies Margetuximab, Trastuzumab or Pertuzumab. 
     The amino acid sequence of the VH Domain of Margetuximab (SEQ ID NO:122 is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLQQSGPE LVKPGASLKL SCTASGFNIK    DTYIH   WVKQR 
               
               
                   
                   
               
               
                   
                 PEQGLEWIG   R       IYPTNGYTRY DPKFQD   KATI TADTSSNTAY 
               
               
                   
                   
               
               
                   
                 LQVSRLTSED TAVYYCSR   WG       GDGFYAMDY   W GQGASVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of Margetuximab (SEQ ID NO:123) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIVMTQSHKF MSTSVGDRVS ITC   KASQDVN TAVA   WYQQKP 
               
               
                   
                   
               
               
                   
                 GHSPKLLIY   S       ASFRYT   GVPD RFTGSRSGTD FTFTISSVQA 
               
               
                   
                   
               
               
                   
                 EDLAVYYC   QQ HYTTPPT   FGG GTKVEIK 
               
            
           
         
       
     
     The amino acid sequences of the complete Heavy and Light Chains of Margetuximab are known in the art (see., e.g., WHO Drug Information, 2014, Recommended INN: List 71, 28(1):93-94). 
     The amino acid sequence of the VH Domain of Trastuzumab (SEQ ID NO:124) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLVESGGG LVQPGGSLRL SCAASGFNIK    DTYIH   WVRQA 
               
               
                   
                   
               
               
                   
                 PGKGLEWVA   R       IYPTNGYTRY ADSVKG   RFTI SADTSKNTAY 
               
               
                   
                   
               
               
                   
                 LQMNSLRAED TAVYYCSR   WG       GDGFYAMDY   W GQGTLVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of Trastuzumab (SEQ ID NO:125) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   RASQDVN TAVA   WYQQKP 
               
               
                   
                   
               
               
                   
                 GKAPKLLIY   S       ASFLY   SGVPS RFSGSRSGTD FTLTISSLQP 
               
               
                   
                   
               
               
                   
                 EDFATYYC   QQ HYTTPPT   FGQ GTKVEIK 
               
            
           
         
       
     
     The amino acid sequence of the VH Domain of Pertuzumab (SEQ ID NO:126) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLVESGGG LVQPGGSLRL SCAASGFTFT    DYTMD   WVRQA 
               
               
                   
                   
               
               
                   
                 PGKGLEWVA   D       VNPNSGGSIY NQRFKG   RFTL SVDRSKNTLY 
               
               
                   
                   
               
               
                   
                 LQMNSLRAED TAVYYCAR   NL       GPSFYFDY   WG QGTLVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of Pertuzumab (SEQ ID NO:127) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   KASQDVS IGVA   WYQQKP 
               
               
                   
                   
               
               
                   
                 GKAPKLLIY   S       ASYRYT   GVPS RFSGSGSGTD FTLTISSLQP 
               
               
                   
                   
               
               
                   
                 EDFATYYC   QQ YYIYPYT   FGQ GTKVEIK 
               
            
           
         
       
     
     In addition to the above-identified preferred anti-HER2/neu Binding Molecules, the invention includes and encompasses CD16 x HER2/neu Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of any of the following anti-HER2/neu Binding Molecules: 1.44.1; 1.140; 1.43; 1.14.1; 1.100.1; 1.96; 1.18.1; 1.20; 1.39; 1.24; and 1.71.3 (U.S. Pat. Nos. 8,350,011; 8,858,942; and PCT Patent Publication WO 2008/019290); F5 and C1 (U.S. Pat. Nos. 7,892,554; 8,173,424; 8,974,792; and PCT Patent Publication WO 99/55367); and also the anti-HER2/neu Binding Molecules of US Patent Publication US2013017114 and PCT Patent Publications WO2011/147986 and WO 2012/143524). 
     The present invention specifically includes and encompasses CD16 x HER2/neu Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of any of Margetuximab, Trastuzumab or Pertuzumab, or any of the other anti-HER2/neu antibodies provided herein; and more preferably possess 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of such anti-HER2/neu monoclonal antibodies. 
     7. Exemplary Anti-VEGF Antibodies 
     VEGF-A is a chemical signal that stimulates angiogenesis in a variety of diseases, especially in certain metastatic cancers such as metastatic colon cancer, and in certain lung cancers, renal cancers, ovarian cancers, and glioblastoma multiforme of the brain. An exemplary antibody that binds to human VEGF-A is “Bevacizumab” (Avastin®). Bevacizumab is a recombinant humanized IgG1 monoclonal antibody (Midgley, R. et al. (2005) “ Bevacizumab—Current Status And Future Directions ,” Ann. Oncol. 16(7):999-1004; Hall, R. D. et al. (2015) “ Angiogenesis Inhibition As A Therapeutic Strategy In Non - Small Cell Lung Cancer  ( NSCLC ),” Transl. Lung Cancer Res. 4(5):515-523; Narita, Y. (2015) “Bevacizumab For Glioblastoma,” Ther. Clin. Risk Manag. 11:1759-1765). 
     The amino acid sequence of the VH Domain of Bevacizumab (SEQ ID NO:128) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
            
               
                 EVQLVESGGG LVQPGGSLRL SCAASGYTFT    NYGMN   WVRQA 
               
               
                   
               
               
                 PGKGLEWVG   W       INTYTGEPTY AADFKR   RFTF SLDTSKSTAY 
               
               
                   
               
               
                 LQMNSLRAED TAVYYCA   KYP       HYYGSSHWYF DV   WGQGTLVT VSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of Bevacizumab (SEQ ID NO:129) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   SASQDIS NYLN   WYQQKP 
               
               
                   
                   
               
               
                   
                 GKAPKVLIY   F       TSSLHS   GVPS RFSGSGSGTD FTLTISSLQP 
               
               
                   
                   
               
               
                   
                 EDFATYYC   QQ YSTVPWT   FGQ GTKVEIKR 
               
            
           
         
       
     
     The present invention specifically includes and encompasses CD16 x VEGF Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L 5 of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-VEGF monoclonal antibody Bevacizumab. 
     8. Exemplary Anti-5T4 Antibodies 
     The oncofetal protein, 5T4, is a tumor-associated protein displayed on the cell membrane of many carcinomas, including kidney, colon, prostate, lung, carcinoma and in acute lymphoblastic leukemia (see, Boghaert, E. R. et al. (2008) “ The Oncofetal Protein,  5 T 4 , Is A Suitable Target For Antibody - Guided Anti - Cancer Chemotherapy With Calicheamicin ,” Int. J. Oncol. 32(1):221-234; Eisen, T. et al. (2014) “ Naptumomab Estafenatox: Targeted Immunotherapy with a Novel Immunotoxin ,” Curr. Oncol. Rep. 16:370, pp. 1-6). Exemplary antibodies that bind to human 5T4 include “5T4 mAb 1” and “5T4 mAb 2.” 
     The amino acid sequence of the VH Domain of 5T4 mAb 1 (SEQ ID NO:130) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLVQSGAE VKKPGASVKV SCKAS   GYTFT       SFWMH   WVRQA 
               
               
                   
                   
               
               
                   
                 PGQGLEWMG   R       IDPNRGGTEY       NEKAKS   RVTM TADKSTSTAY 
               
               
                   
                   
               
               
                   
                 MELSSLRSED TAVYYCAG   GN       PYYPMDY   WGQ GTTVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of an 5T4 mAb 1 (SEQ ID NO:131) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   RASQGIS       NYLA   WFQQKP 
               
               
                   
                   
               
               
                   
                 GKAPKSLIY   R       ANRLQS   GVPS RFSGSGSGTD FTLTISSLQP 
               
               
                   
                   
               
               
                   
                 EDVATYYC   LQ       YDDFPWT   FGQ GTKLEIK 
               
            
           
         
       
     
     The amino acid sequence of the VH Domain of 5T4 mAb 2 (SEQ ID NO:132) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
            
               
                 QVQLQQPGAE LVKPGASVKM SCKAS   GYTFT       SYWIT   WVKQR 
               
               
                   
               
               
                 PGQGLEWIG   D       IYPGSGRANY       NEKFKS   KATL TVDTSSSTAY 
               
               
                   
               
               
                 MQLSSLTSED SAVYNCAR   YG       PLFTTVVDPN       SYAMDY   WGQG TSVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of 5T4 mAb 2 (SEQ ID NO:133) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DVLMTQTPLS LPVSLGDQAS ISC   RSSQSIV       YSNGNTYLE   W 
               
               
                   
                   
               
               
                   
                 YLQKPGQSPK LLIY   KVSNRF       S   GVPDRFSGS GSGTDFTLKI 
               
               
                   
                   
               
               
                   
                 SRVEAEDLGV YYC   FQGSHVP       FT   FGSGTKLE IK 
               
            
           
         
       
     
     The present invention specifically includes and encompasses CD16 x 5T4 Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-5T4 monoclonal antibodies 5T4 mAb 1 or 5T4 mAb 2, or of any of the anti-5T4 antibodies provided in WO 2013/041687 or WO 2015/184203. 
     The present application additionally specifically includes and encompasses CD16 x 5T4 Trispecific Binding Molecules that are capable of binding to 5T4, to CD16 and to CD8, and particularly such trispecific binding molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-5T4 monoclonal antibodies 5T4 mAb 1 or 5T4 mAb 2 or of any of the anti-5T4 monoclonal antibodies provided in WO 2015/184203, and/or the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of any of the anti-CD8 monoclonal antibodies provided herein. 
     9. Exemplary Anti-IL13Rα2 Antibodies 
     Interleukin-13 Receptor α2 (IL13Rα2) is overexpressed in a variety of cancers, including glioblastoma, colorectal cancer, cervical cancer, pancreatic cencer, multiple melanoma, osteosarcoma, leukemia, lymphoma, prostate cancer and lung cancer (PCT Pubmication No. WO 2008/146911; Brown, C. E. et al. (2013) “ Glioma IL 13 Rα 2  Is Associated With Mesenchymal Signature Gene Expression And Poor Patient Prognosis ,” PLoS One. 18; 8(10):e77769; Barderas, R. et al. (2012) “ High Expression Of IL -13  Receptor A 2  In Colorectal Cancer Is Associated With Invasion, Liver Metastasis, And Poor Prognosis ,” Cancer Res. 72(11):2780-2790; Kasaian, M. T. et al. (2011) “ IL -13  Antibodies Influence IL -13  Clearance In Humans By Modulating Scavenger Activity Of IL -13 Rα 2,” J. Immunol. 187(1):561-569; Bozinov, O. et al. (2010) “ Decreasing Expression Of The Interleukin -13  Receptor IL -13 Ralpha 2  In Treated Recurrent Malignant Gliomas ,” Neurol. Med. Chir. (Tokyo) 50(8):617-621; Fujisawa, T. et al. (2009) “ A Novel Role Of Interleukin -13  Receptor Alpha 2  In Pancreatic Cancer Invasion And Metastasis ,” Cancer Res. 69(22):8678-8685). Antibodies that immunospecifically bind to IL13Rα2 are commercially available and have been described in the art (Abnova Corporation, Biorbyt, LifeSpan BioSciences, United States Biologicals; see also PCT Publication No. WO 2008/146911). Exemplary antibodies that bind to human IL13Rα2 include “hu08” (see, e.g., PCT Publication No. WO 2014/072888). 
     The amino acid sequence of the VH Domain of hu08 (SEQ ID NO:134) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLVESGGG LVQPGGSLRL SCAAS   GFTFS RNGMS   WVRQA 
               
               
                   
                   
               
               
                   
                 PGKGLEWVA   T       VSSGGSYIYY ADSVKG   RFTI SRDNAKNSLY 
               
               
                   
                   
               
               
                   
                 LQMNSLRAED TAVYYCAR   QG       TTALATRFFD V   WGQGTLVTV SS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of hu08 (SEQ ID NO:135) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITC   KASQDVG TAVA   WYQQKP 
               
               
                   
                   
               
               
                   
                 GKAPKLLIY   S       ASYRST   GVPS RFSGSGSGTD FTLTISSLQP 
               
               
                   
                   
               
               
                   
                 EDFATYYC   QH HYSAPWT   FGG GTKVEIK 
               
            
           
         
       
     
     The present invention specifically includes and encompasses CD16 x IL13Rα2 Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-IL13Rα2 monoclonal antibody hu08. 
     10. Exemplary Anti-CD123 Antibodies 
     CD123 (interleukin 3 receptor alpha, IL-3Ra) is a 40 kDa molecule and is part of the interleukin 3 receptor complex (Stomski, F. C. et al. (1996) “ Human Interleukin -3 ( IL -3)  Induces Disulfide - Linked IL -3  Receptor Alpha - And Beta - Chain Heterodimerization, Which Is Required For Receptor Activation But Not High - Affinity Binding ,” Mol. Cell. Biol. 16(6):3035-3046). Interleukin 3 (IL-3) drives early differentiation of multipotent stem cells into cells of the erythroid, myeloid and lymphoid progenitors. CD123 has been reported to be overexpressed on malignant cells in a wide range of hematologic malignancies including acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) (Muñoz, L. et al. (2001) “ Interleukin -3  Receptor Alpha Chain  ( CD 123)  Is Widely Expressed In Hematologic Malignancies ,” Haematologica 86(12):1261-1269). Overexpression of CD123 is associated with poorer prognosis in AML (Tettamanti, M. S. et al. (2013) “ Targeting Of Acute Myeloid Leukaemia By Cytokine - Induced Killer Cells Redirected With A Novel CD 123- Specific Chimeric Antigen Receptor ,” Br. J. Haematol. 161:389-401). 
     An exemplary antibody that binds to human CD123, and that may be employed in the present invention, is “CD123 mAb 1” (see, e.g., PCT Patent Publication WO 2015/026892). 
     The amino acid sequence of the VH Domain of CD123 mAb 1 (SEQ ID NO:136) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 EVQLVQSGAE LKKPGASVKV SCKASGYTFT    DYYMK   WVRQA 
               
               
                   
                   
               
               
                   
                 PGQGLEWIG   D       IIPSNGATFY       NQKFKG   RVTI TVDKSTSTAY 
               
               
                   
                   
               
               
                   
                 MELSSLRSED TAVYYCAR   SH       LLRASWFAY   W GQGTLVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of CD123 mAb 1 (SEQ ID NO:137) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DFVMTQSPDS LAVSLGERVT MSC   KSSQSLL NSGNQKNYLT     
               
               
                   
                   
               
               
                   
                 WYQQKPGQPP KLLIY   WASTR ES   GVPDRFSG SGSGTDFTLT 
               
               
                   
                   
               
               
                   
                 ISSLQAEDVA VYYC   QNDYSY       PYT   FGQGTKL EIK 
               
            
           
         
       
     
     The present invention specifically includes and encompasses CD16 x CD123 Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-CD123 monoclonal antibody CD123 mAb 1 or any of the anti-CD123 antibodies disclosed in US 2017/081424 and WO 2016/036937. 
     11. Exemplary Anti-CD19 Antibodies 
     CD19 (B lymphocyte surface antigen B4, Genbank accession number M28170) is a component of the B cell-receptor (BCR) complex, and is a positive regulator of B cell signaling that modulates the threshold for B cell activation and humoral immunity. CD19 is one of the most ubiquitously expressed antigens in the B cell lineage and is expressed on &gt;95% of B cell malignancies, including acute lymphoblastic leukemia (ALL), chronic lymphocytic leukemia (CLL), and non-Hodgkin&#39;s Lymphoma (NHL). Notably, CD19 expression is maintained on B cell lymphomas that become resistant to anti-CD20 therapy (Davis et al. (1999) “ Therapy of B - cell Lymphoma With Anti - CD 20  Antibodies Can Result In The Loss Of CD 20  Antigen Expression .” Clin Cancer Res, 5:611-615, 1999). CD19 has also been suggested as a target to treat autoimmune diseases (Tedder (2009) “ CD 19 : A Promising B Cell Target For Rheumatoid Arthritis ,” Nat. Rev. Rheumatol. 5:572-577). 
     An exemplary antibody that binds to human CD19, and that may be employed in the present invention, is the anti-CD19 antibody disclosed in WO 2016/048938 (referred to herein as “CD19 mAb 1”). 
     The amino acid sequence of the VH Domain of CD19 mAb 1 (SEQ ID NO:123) is shown below (CDR H  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVTLRESGPA LVKPTQTLTL TCTFSGFSLS    TSGMGVG   WIR 
               
               
                   
                   
               
               
                   
                 QPPGKALEWL A   HIWWDDDKR       YNPALKS   RLT ISKDTSKNQV 
               
               
                   
                   
               
               
                   
                 FLTMTNMDPV DTATYYCAR   M       ELWSYYFDY   W GQGTTVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of CD19 mAb 1 (SEQ ID NO:139) is shown below (CDR L  residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 ENVLTQSPAT LSVTPGEKAT ITC   RASQSVS YMH   WYQQKPG 
               
               
                   
                   
               
               
                   
                 QAPRLLIY   DA       SNRAS   GVPSR FSGSGSGTDH TLTISSLEAE 
               
               
                   
                   
               
               
                   
                 DAATYYC   FQG SVYPF     T FGQG TKLEIK 
               
            
           
         
       
     
     The present invention specifically includes and encompasses CD16 x CD19 Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L 5 of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-CD19 monoclonal antibody CD19 mAb 1, or any of the anti-CD19 antibodies disclosed in U.S. Pat. No. 7,112,324. 
     B. Exemplary Pathogen-Associated Antigens 
     As used herein, the term “Pathogen-Associated Antigen” denotes an antigen that is characteristically expressed on the surface of a pathogen-infected cell, and that may thus be treated with an Antibody-Based Molecule or an Immunomodulatory Molecule. Examples of Pathogen-Associated Antigens include, but are not limited to antigens expressed on the surface of a cell infected with: a Herpes Simplex Virus (e.g., infected cell protein (ICP)47, gD, etc.), a varicella-zoster virus, a Kaposi&#39;s sarcoma-associated herpesvirus, an Epstein-Barr Virus (e.g., LMP-1, LMP-2A, LMP-2B, etc.), a Cytomegalovirus (e.g., UL11, etc.), Human Immunodeficiency Virus (e.g., env proteins gp160, gp120, gp41, etc.), a Human Papillomavirus (e.g., E6, E7, etc.), a human T-cell leukemia virus (e.g., env proteins gp64, gp46, gp21, etc.), Hepatitis A Virus, Hepatitis B Virus, Hepatitis C Virus, Vesicular Stomatitis Virus (VSV), Bacilli,  Citrobacter , Cholera, Diphtheria,  Enterobacter , Gonococci,  Helicobacter pylori, Klebsiella, Legionella , Meningococci, mycobacteria,  Pseudomonas , Pneumonococci,  rickettsia  bacteria,  Salmonella, Serratia , Staphylococci, Streptococci, Tetanus,  Aspergillus  ( fumigatus, niger , etc.),  Blastomyces dermatitidis, Candida  ( albicans, krusei, glabrata, tropicalis , etc.),  Cryptococcus neoformans , Genus Mucorales ( mucor, absidia, rhizopus ),  Sporothrix schenkii, Paracoccidioides brasiliensis, Coccidioides immitis, Histoplasma capsulatum , Leptospirosis,  Borrelia burgdorferi , helminth parasite (hookworm, tapeworms, flukes, flatworms (e.g. Schistosomia), Giardia Zambia,  trichinella, Dientamoeba Fragilis, Trypanosoma brucei, Trypanosoma cruzi , and  Leishmania donovani ). Such antibodies are available commercially from a wide number of sources, or can be obtained by immunizing mice or other animals (including for the production of monoclonal antibodies) with such antigens. 
     Exemplary antibodies, whose VH and VL Domains may be used to construct molecules capable of binding a Pathogen-Associated Antigen arrayed on the surface of a pathogen-infected cell are antibodies are provided below, additional antibodies are known in the art. 
     1. Exemplary Anti-HIV Antibodies 
     The env protein of HIV is an exemplary Pathogen-Associated Antigen, and antibodies that bind the env protein of HIV are exemplary of antibodies capable of binding a Pathogen-Associated Antigen. 
     The initial step in HIV-1 infection occurs with the binding of cell surface CD4 to trimeric HIV-1 envelope glycoproteins (env), a heterodimer of a transmembrane glycoprotein (gp41) and a surface glycoprotein (gp120). The gp120 and gp41 glycoproteins are initially synthesized as a single gp160 polypeptide that is subsequently cleaved to generate the non-covalently associated gp120/gp41 complex. The ectodomain of env is a heterodimer with mass of approximately 140 kDa, composed of the entire gp120 component, and approximately 20 kDa of gp41 (Harris, A. et al. (2011) “ Trimeric HIV -1  Glycoprotein Gp 140  Immunogens And Native HIV -1  Envelope Glycoproteins Display The Same Closed And Open Quaternary Molecular Architectures ,” Proc. Natl. Acad. Sci. (U.S.A.) 108(28):11440-11445). Antibodies that that immunospecifically bind to env proteins are commercially available and have been described in the art (see, e.g., GenBank Accession No. AFQ31503; Buchacher, A. et al. (1994) “ Generation Of Human Monoclonal Antibodies Against HIV -1  Proteins; Electrofusion And Epstein - Barr Virus Transformation For Peripheral Blood Lymphocyte Immortalization ,” AIDS Res. Hum. Retroviruses 10(4):359-369; Shen, R. (2010) “ GP 41- Specific Antibody Blocks Cell - Free HIV Type  1  Transcytosis Through Human Rectal Mucosa And Model Colonic Epithelium ,” J. Immunol. 184(7):3648-3655; WO 2012/162068; and WO 2016/054101). Exemplary antibodies that bind to HIV env include “7B2” (GenBank Accession No. AFQ31503) and “A32” (PCT Publication No. WO 2014/159940). 
     Antibody 7B2 (Genbank accession numbers JX188438 and JX188439) is an anti-HIV env human IgG1 antibody that binds HIV gp41 at 598-604 in the immunodominant helix-loop-helix region of the molecule (Sadraeian, M. et al. (2017) “ Selective Cytotoxicity Of A Novel Immunotoxin Based On Pulchellin A Chain For Cells Expressing HIV Envelope ,” Sci. Rep. 7(1):7579 doi: 10.1038/s41598-017-08037-3). The antibody was isolated from an HIV-1 chronically infected subject using Epstein-Barr (EB) virus B cell transformation and heterohybridoma production (Pincus, S. H. et al. (2003) “ In Vivo Efficacy Of Anti - Glycoprotein  41 , But Not Anti - Glycoprotein  120 , Immunotoxins In A Mouse Model Of HIV Infection ,” J. Immunol. 170(4):2236-2241). Antibody 7B2 has been found to be capable of recognizing both virus particles and infected cells (Santra, S. et al. (2015) “ Human Non - neutralizing HIV -1  Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques ,” PLoS Pathog. 11(8):e1005042. doi: 10.1371/journal.ppat.1005042; Tay, M. Z. et al. (2016) “ Antibody - Mediated Internalization of Infectious HIV -1  Virions Differs among Antibody Isotypes and Subclasses ,” PLoS Pathog. 12(8):e1005817. doi: 10.1371/journal.ppat.1005817). 
     The amino acid sequence of the VH Domain of 7B2 (SEQ ID NO:140) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
            
               
                 QVQLVQSGGG VFKPGGSLRL SCEASGFTFT    EYYMT   WVRQA 
               
               
                   
               
               
                 PGKGLEWLAY    ISKNGEYSKY       SPSSNG   RFTI SRDNAKNSVF 
               
               
                   
               
               
                 LQLDRLSADD TAVYYCAR   AD       GLTYFSELLQ       YIFDL   WGQGA RVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of 7B2 (SEQ ID NO:141) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIVMTQSPDS LAVSPGERAT IHCK   SSQTLL       YSSNNRHSIA     
               
               
                   
               
               
                   
                 WYQQRPGQPP KLLLY   WASMR       LS   GVPDRFSG SGSGTDFTLT 
               
               
                   
               
               
                   
                 INNLQAEDVA IYYC   HQYSSH       PPT   FGHGTRV EIK 
               
            
           
         
       
     
     Monoclonal antibody A32 recognizes a conformational epitope in the C1 region of HIV-1 Env gp120 (Wyatt et al. (1995) “ Involvement Of The V 1 /V 2  Variable Loop Structure In The Exposure Of Human Immunodeficiency Virus Type  1  gp 120  Epitopes Induced By Receptor Binding ,” J. Virol. 69:5723-5733) and mediates potent ADCC activity and could block a significant proportion of ADCC-mediating Ab activity detectable in HIV-1 infected individuals (Ferrari, G. et al. (2011) “ An HIV -1  gp 120  Envelope Human Monoclonal Antibody That Recognizes a C 1  Conformational Epitope Mediates Potent Antibody Dependent Cellular Cytotoxicity  ( ADCC )  Activity and Defines a Common ADCC Epitope in Human HIV -1  Serum ,” J. Virol. 85(14): 7029-7036). 
     Multiple VH Domains of Antibody A32 have been reported in the art that possess minor changes in framework regions 1 and/or 4 reported (see, e.g., Protein Data Base Accession number PDB: 4YBL_H, US 2015/0239961 and WO 2006/044410). Any of these variant Antibody A32 VH Domains may be employed in accordance with the present invention. The amino acid sequence of an illustrative VH Domain of A32 (SEQ ID NO:142) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVQLQESGPG LVKPSQTLSL SCTVSGGSSS    SGAHYWS   WIR 
               
               
                   
               
               
                   
                 QYPGKGLEWI G   YIHYSGNTY       YNPSLKS   RIT ISQHTSENQF 
               
               
                   
               
               
                   
                 SLKLNSVTVA DTAVYYCAR   G       TRLRTLRNAF DI   WGQGTLVT 
               
               
                   
               
               
                   
                 VSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of A32 (SEQ ID NO:143) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QSALTQPPSA SGSPGQSVTI SC   TGTSSDVG GYNYVS   WYQH 
               
               
                   
               
               
                   
                 HPGKAPKLII S   EVNNRPS   GV PDRFSGSKSG NTASLTVSGL 
               
               
                   
               
               
                   
                 QAEDEAEYYC    SSYTDIHNFV    FGGGTKLTVL 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of A32 (SEQ ID NO:143) may be employed with the illustrative VH Domain of A32 (SEQ ID NO:142) or with any of the variant Antibody A32 VH Domains (see, e.g., Protein Data Base Accession number PDB: 4YBL_H, US 2015/0239961 and WO 2006/044410) to form an anti-HIV-1 Env gp120 Epitope Binding Site. 
     The present invention specifically includes and encompasses CD16 x HIV Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-HIV monoclonal antibodies 7B2 or A32, or of any of the anti-HIV antibodies disclosed in WO 2016196975, WO 2016/149710, WO 2016/149698, WO 2016/149695, WO 2015/048610, WO 2012/030904, WO 2013/163427, WO 2013/192589, WO 2014/063059, WO 20170/11413, WO 2016/054101, WO 2014/159940, or WO 2017/011414. 
     The present application additionally specifically includes and encompasses CD16 x HIV Binding Molecules that are capable of binding to HIV, to CD16 and to CD8, and particularly such trispecific binding molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-HIV monoclonal antibodies 7B2 or A32 or of any of the anti-HIV monoclonal antibodies provided in WO 2015/184203, WO 2016/054101, WO 2017/011413, WO 2017/011414. 
     2. Exemplary Anti-RSV Antibody 
     A further illustrative Pathogen-Associated Antigen is RSV glycoprotein F. An exemplary anti-RSV glycoprotein F antibody is Palivizumab (see, e.g., Protein Data Bank (PDB) ID No. 2HWZ). Alternative anti-RSV glycoprotein F antibodies include motavizumab (see, e.g., PDB ID No. 3IXT) and a variant of palivizumab (also referred to herein as “vPalivizumab”) that has been engineered to remove a cysteine residue from palivizumab&#39; s CDR L 1. The amino acid sequence of the VH Domain of the variant of palivizumab (SEQ ID NO:144) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 QVTLRESGPA LVKPTQTLTL TCTFSGFSLS    TSGMSVG   WIR 
               
               
                   
               
               
                   
                 QPPGKALEWL A   DIWWDDKKD       YNPSLKS   RLT ISKDTSKNQV 
               
               
                   
               
               
                   
                 VLKVTNMDPA DTATYYCAR   S       MITNWYFDV   W GAGTTVTVSS 
               
            
           
         
       
     
     The amino acid sequence of the VL Domain of the variant of palivizumab (SEQ ID NO:145) is shown below (CDR residues are shown underlined): 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPST LSASVGDRVT ITC   RASQSVG       YMH   WYQQKPG 
               
               
                   
               
               
                   
                 KAPKLLIY   DT       SKLAS   GVPSR FSGSGSGTEF TLTISSLQPD 
               
               
                   
               
               
                   
                 DFATYYC   FQG       SGYPFT   FGGG TKLEIK 
               
            
           
         
       
     
     The present invention specifically includes and encompasses CD16 x RSV Binding Molecules that comprise the VL and/or VH Domain, and/or 1, 2 or all 3 of the CDR L s of the VL Region and/or 1, 2 or all 3 of the CDR H s of the VH Domain of the anti-RSV monoclonal antibody palivizumab or vPalivizumab. 
     VII. Exemplary Binding Molecules of the Present Invention 
     The principles of the present invention are illustrated by a series of exemplary CD16 x DA Binding Molecules incorporating different murine or humanized anti-CD16 binding domains and having a Binding Domain that is immunospecific for a Disease Antigen. The covalent diabody structures and sequences of such illustrative CD16 x DA Binding Molecules are summarized in Table 12, and are described in detail below. As will be recognized, analogous diabodies and other bispecific molecules may likewise be constructed (by employing the VL and VH domains of desired antibodies in lieu of the VL and VH domains used in the illustrative CD16 x DA Binding Molecules. 
     
       
         
           
               
               
               
               
               
               
               
               
               
             
               
                 TABLE 12 
               
               
                   
               
               
                 Diabody 
                 Polypeptide 
                 SEQ ID 
                   
                   
                 SEQ ID 
                   
                   
                 SEQ ID 
               
               
                 Name 
                 Chain 
                 NO 
                 Antibody 
                 Domain 
                 NO 
                 Antibody 
                 Domain 
                 NO 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-A 
                 1 
                 151 
                 Trastuzumab 
                 VL 
                 125 
                 CD16-M1 
                 VH 
                 64 
               
               
                   
                 2 
                 152 
                 CD16-M1 
                 VL 
                 65 
                 Trastuzumab 
                 VH 
                 124 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-B 
                 1 
                 154 
                 Trastuzumab 
                 VL 
                 125 
                 CD16-M2 
                 VH 
                 75 
               
               
                   
                 2 
                 155 
                 CD16-M2 
                 VL 
                 76 
                 Trastuzumab 
                 VH 
                 124 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-C 
                 1 
                 156 
                 Trastuzumab 
                 VL 
                 125 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 157 
                 hCD16-M1 
                 VL 
                 73 
                 Trastuzumab 
                 VH 
                 124 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-D 
                 1 
                 158 
                 Trastuzumab 
                 VL 
                 125 
                 hCD16-M2 
                 VH1 
                 83 
               
               
                   
                 2 
                 159 
                 hCD16-M2 
                 VL 
                 85 
                 Trastuzumab 
                 VH 
                 124 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-E 
                 1 
                 160 
                 Trastuzumab 
                 VL 
                 125 
                 hCD16-M2 
                 VH2 
                 84 
               
               
                   
                 2 
                 159 
                 hCD16-M2 
                 VL 
                 85 
                 Trastuzumab 
                 VH 
                 124 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-F 
                 1 
                 161 
                 A32 
                 VL 
                 143 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 162 
                 hCD16-M1 
                 VL 
                 73 
                 A32 
                 VH 
                 142 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-G 
                 1 
                 163 
                 A32 
                 VL 
                 143 
                 hCD16-M2 
                 VH1 
                 83 
               
               
                   
                 2 
                 164 
                 hCD16-M2 
                 VL 
                 85 
                 A32 
                 VH 
                 142 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-H 
                 1 
                 165 
                 7B2 
                 VL 
                 141 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 166 
                 hCD16-M1 
                 VL 
                 73 
                 7B2 
                 VH 
                 140 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-I 
                 1 
                 186 
                 Trastuzumab 
                 VL 
                 125 
                 hCD16-M1A 
                 VH 
                 58 
               
               
                   
                 2 
                 157 
                 hCD16-M1 
                 VL 
                 73 
                 Trastuzumab 
                 VH 
                 124 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-J 
                 1 
                 156 
                 Trastuzumab 
                 VL 
                 125 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 187 
                 hCD16-M1B 
                 VL 
                 59 
                 Trastuzumab 
                 VH 
                 124 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-K 
                 1 
                 186 
                 Trastuzumab 
                 VL 
                 125 
                 hCD16-M1A 
                 VH 
                 58 
               
               
                   
                 2 
                 187 
                 hCD16-M1B 
                 VL 
                 59 
                 Trastuzumab 
                 VH 
                 124 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-L 
                 1 
                 188 
                 CD19 mAb 1 
                 VL 
                 139 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 189 
                 hCD16-M1 
                 VL 
                 73 
                 CD19 mAb 1 
                 VH 
                 138 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-M 
                 1 
                 188 
                 CD19 mAb 1 
                 VL 
                 139 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 190 
                 hCD16-M1B 
                 VL 
                 59 
                 CD19 mAb 1 
                 VH 
                 138 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-N 
                 1 
                 191 
                 CD19 mAb 1 
                 VL 
                 139 
                 hCD16-M1A 
                 VH 
                 58 
               
               
                   
                 2 
                 190 
                 hCD16-M1B 
                 VL 
                 59 
                 CD19 mAb 1 
                 VH 
                 138 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-1 
                 1 
                 167 
                 Trastuzumab 
                 VL 
                 125 
                 h3G8 
                 VH 
                 62 
               
               
                   
                 2 
                 168 
                 h3G8 
                 VL 
                 63 
                 Trastuzumab 
                 VH 
                 124 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-2 
                 1 
                 — 
                 vPalivizumab 
                 VL 
                 145 
                 h3G8 
                 VH 
                 62 
               
               
                   
                 2 
                 — 
                 h3G8 
                 VL 
                 63 
                 vPalivizumab 
                 VH 
                 144 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-3 
                 1 
                 169 
                 vPalivizumab 
                 VL 
                 145 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 170 
                 hCD16-M1 
                 VL 
                 73 
                 vPalivizumab 
                 VH 
                 144 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-4 
                 1 
                 — 
                 7B2 
                 VL 
                 141 
                 h3G8 
                 VH 
                 62 
               
               
                   
                 2 
                 — 
                 h3G8 
                 VL 
                 63 
                 7B2 
                 VH 
                 140 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-5 
                 1 
                 — 
                 vPalivizumab 
                 VL 
                 145 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 — 
                 hCD16-M1 
                 VL 
                 73 
                 vPalivizumab 
                 VH 
                 144 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-6 
                 1 
                 — 
                 vPalivizumab 
                 VL 
                 145 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 — 
                 hCD16-M1B 
                 VL 
                 59 
                 vPalivizumab 
                 VH 
                 144 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-7 
                 1 
                 — 
                 vPalivizumab 
                 VL 
                 145 
                 hCD16-M1A 
                 VH 
                 58 
               
               
                   
                 2 
                 — 
                 hCD16-M1B 
                 VL 
                 59 
                 vPalivizumab 
                 VH 
                 144 
               
            
           
           
               
               
               
               
            
               
                   
                 3 
                 153 
                 Common Diabody Polypeptide Chain 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 DART-X 
                 1 
                 171 
                 7B2 
                 VL 
                 141 
                 CD16-M1 
                 VH 
                 64 
               
               
                   
                 2 
                 172 
                 CD16-M1 
                 VL 
                 65 
                 7B2 
                 VH 
                 140 
               
               
                 DART-Y 
                 1 
                 173 
                 7B2 
                 VL 
                 141 
                 CD16-M2 
                 VH 
                 75 
               
               
                   
                 2 
                 174 
                 CD16-M2 
                 VL 
                 76 
                 7B2 
                 VH 
                 140 
               
               
                 DART-Z 
                 1 
                 — 
                 A32 
                 VL 
                 143 
                 4-LSN1 
                 VH 
                 175 
               
               
                   
                 2 
                 — 
                 4-LSN1 
                 VL 
                 176 
                 A32 
                 VH 
                 142 
               
               
                 DART-0 
                 1 
                 — 
                 7B2 
                 VL 
                 141 
                 h3G8 
                 VH 
                 62 
               
               
                   
                 2 
                 — 
                 h3G8 
                 VL 
                 63 
                 7B2 
                 VH 
                 140 
               
               
                   
               
            
           
         
       
     
     A. CD16 x HER2/neu Binding Molecule, DART-A 
     The CD16 x HER2/neu Binding Molecule designated “DART-A” is a first illustrative CD16 x DA Binding Molecule. DART-A is an Fc Domain-containing, bispecific diabody capable of binding CD16 and the HER2/neu cancer antigen. DART-A is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of anti-human CD16 antibody CD16-M1 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of Trastuzumab (and is thus immunospecific for an epitope of the HER2/neu Cancer Antigen). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HER2/neu Cancer Antigen (see, e.g.,  FIG. 4A ). 
     The first polypeptide chain of DART-A has the amino acid sequence of SEQ ID NO:151: 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITCRASQDVN TAVAWYQQKP 
               
               
                   
               
               
                   
                 GKAPKLLIYS ASFLYSGVPS RFSGSRSGTD FTLTISSLQP 
               
               
                   
               
               
                   
                 EDFATYYCQQ HYTTPPTFGQ GTKVEIK   GGG       SGGGG   EVKLV 
               
               
                   
               
               
                   
                 ESGGTLVKPG GSLKLSCAAS GFTFNNYGMS WVRQTPEKRL 
               
               
                   
               
               
                   
                 EWVATISGGG SYTFYPDSVK GRFTISRDNA KNSLYLQMSS 
               
               
                   
               
               
                   
                 LRSEDTALYY CIRQSARAPE PYWGQGTLVT VSS   ASTKG   EV 
               
               
                   
               
               
                   
                 AACEKEVAAL EKEVAALEKE VAALEKGGGD KTHTCPPCPA 
               
               
                   
               
               
                   
                 PEAAGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP 
               
               
                   
               
               
                   
                 EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ 
               
               
                   
               
               
                   
                 DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL 
               
               
                   
               
               
                   
                 PPSREEMTKN QVSLWCLVKG FYPSDIAVEW ESNGQPENNY 
               
               
                   
               
               
                   
                 KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA 
               
               
                   
               
               
                   
                 LHNHYTQKSL SLSPGK 
               
            
           
         
       
     
     Residues 1-107 of the first polypeptide chain (SEQ ID NO:151) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Trastuzumab (SEQ ID NO:125). Residues 108-115 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-233 of the first polypeptide chain correspond to the VH Domain of anti-human CD16 antibody CD16-M1 (SEQ ID NO:64). Residues 234-238 correspond to a linker (SEQ ID NO:21, underlined). Residues 239-266 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 267-279 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 280-496 of the first polypeptide chain correspond to a “knob-bearing” (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of DART-A has the amino acid sequence of SEQ ID NO:152: 
     
       
         
           
               
               
            
               
                   
                 DIVMTQSQKF MSTSVGDRVS VTCKASQNVG THVAWYQQKS 
               
               
                   
               
               
                   
                 GQSPKSLLYS ASYRYSGVPD RFSGSGSGTD FTLTISNVQS 
               
               
                   
               
               
                   
                 EDLAEYFCQQ YKSYPLTFGA GTKLELK   GGG       SGGGG   EVQLV 
               
               
                   
               
               
                   
                 ESGGGLVQPG GSLRLSCAAS GFNIKDTYIH WVRQAPGKGL 
               
               
                   
               
               
                   
                 EWVARIYPTN GYTRYADSVK GRFTISADTS KNTAYLQMNS 
               
               
                   
               
               
                   
                 LRAEDTAVYY CSRWGGDGFY AMDYWGQGTL VTVSS   ASTKG     
               
               
                   
               
               
                   
                 KVAACKEKVA ALKEKVAALK EKVAALKE 
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:152) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of anti-human CD16 antibody CD16-M1 (SEQ ID NO:65). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-235 of the second polypeptide chain correspond to the VH Domain of Trastuzumab (SEQ ID NO:124). Residues 236-240 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 241-268 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of the DART-A has the amino acid sequence of SEQ ID NO:153: 
     
       
         
           
               
               
            
               
                   
                 DKTHTCPPCP APEAAGGPSV FLFPPKPKDT LMISRTPEVT 
               
               
                   
               
               
                   
                 CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY 
               
               
                   
               
               
                   
                 RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK 
               
               
                   
               
               
                   
                 GQPREPQVYT LPPSREEMTK NQVSLSCAVK GFYPSDIAVE 
               
               
                   
               
               
                   
                 WESNGQPENN YKTTPPVLDS DGSFFLVSKL TVDKSRWQQG 
               
               
                   
               
               
                   
                 NVFSCSVMHE ALHN   R   YTQKS LSLSPGK 
               
            
           
         
       
     
     Residues 1-10 of the third polypeptide chain (SEQ ID NO:153) of such illustrative CD16 x DA Binding Molecule correspond to a linker (SEQ ID NO:40). Residues 11-227 of the third polypeptide chain correspond to a “hole-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:53), containing the H435R substitution (shown underlined), and in which the final residue is lysine. As stated above, the H435R substitution eliminates the ability of the molecule to bind to bind protein A. 
     As will be recognized, the third polypeptide chain of DART-A does not contain any Epitope Binding sites and may thus be employed in various CD16 x DA Binding Molecules. Accordingly, the third polypeptide chain of DART-A is referred to as a “Common Diabody Polypeptide Chain.” 
     B. CD16 x HER2/neu Binding Molecule, “DART-B” 
     The CD16 x Her2/neu Binding Molecule designated “DART-B” is a further illustrative CD16 x DA Binding Molecule. DART-B is an Fc Domain-containing, bispecific diabody capable of binding CD16 and the HER2/neu Cancer Antigen. DART-B is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of anti-human CD16 antibody CD16-M2 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of Trastuzumab (and is thus immunospecific for an epitope of the HER2/neu Cancer Antigen). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HER2/neu Cancer Antigen (see, e.g.,  FIG. 4A ). 
     The first polypeptide chain of DART-B has the amino acid sequence of SEQ ID NO:154: 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITCRASQDVN TAVAWYQQKP 
               
               
                   
               
               
                   
                 GKAPKLLIYS ASFLYSGVPS RFSGSRSGTD FTLTISSLQP 
               
               
                   
               
               
                   
                 EDFATYYCQQ HYTTPPTFGQ GTKVEIK     EVQLQ 
               
               
                   
               
               
                   
                 QSGPELVKPG ASVKMSCKAS GYTFTSSAMH WVKKNPGQGL 
               
               
                   
               
               
                   
                 EWIGYINHYN DGIKYNERFK GKATLTSDKS SSTAYMELSS 
               
               
                   
               
               
                   
                 LTSEDSAVYY CATGYRYASW FASWGQGTLV TVSS   ASTKG   E 
               
               
                   
               
               
                   
                 VAACEKEVAA LEKEVAALEK EVAALEKGGG DKTHTCPPCP 
               
               
                   
               
               
                   
                 APEAAGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED 
               
               
                   
               
               
                   
                 PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 
               
               
                   
               
               
                   
                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 
               
               
                   
               
               
                   
                 LPPSREEMTK NQVSLWCLVK GFYPSDIAVE WESNGQPENN 
               
               
                   
               
               
                   
                 YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE 
               
               
                   
               
               
                   
                 ALHNHYTQKS LSLSPGK 
               
            
           
         
       
     
     Residues 1-107 of the first polypeptide chain (SEQ ID NO:154) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Trastuzumab (SEQ ID NO:125). Residues 108-115 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-234 of the first polypeptide chain correspond to the VH Domain of anti-human CD16 antibody CD16-M2 (SEQ ID NO:75). Residues 235-239 correspond to a linker (SEQ ID NO:21, underlined). Residues 240-267 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 268-280 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 281-497 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of DART-B has the amino acid sequence of SEQ ID NO:155: 
     
       
         
           
               
               
            
               
                   
                 DILLTQSPAI LSVSPGERVS FSCRASQNIG TSIHWYQQRT 
               
               
                   
               
               
                   
                 DGSPRLLIKS VSESISGIPS RFSGSGSGTD FTLTINGVES 
               
               
                   
               
               
                   
                 GDISDYYCQQ SNSWPLTFGA GTKLELK     EVQLV 
               
               
                   
               
               
                   
                 ESGGGLVQPG GSLRLSCAAS GFNIKDTYIH WVRQAPGKGL 
               
               
                   
               
               
                   
                 EWVARIYPTN GYTRYADSVK GRFTISADTS KNTAYLQMNS 
               
               
                   
               
               
                   
                 LRAEDTAVYY CSRWGGDGFY AMDYWGQGTL VTVSS   ASTKG     
               
               
                   
               
               
                   
                 KVAACKEKVA ALKEKVAALK EKVAALKE 
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:155) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of anti-human CD16 antibody CD16-M2 (SEQ ID NO:76). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-235 of the second polypeptide chain correspond to the VH Domain of Trastuzumab (SEQ ID NO:124). Residues 236-240 of the second polypeptide chain correspond to a linker (SEQ ID NO: 21, underlined). Residues 241-268 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of DART-B has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     C. CD16 x HER2/neu Binding Molecule “DART-C” 
     The CD16 x HER2/neu Binding Molecule designated “DART-C” is a further illustrative CD16 x DA Binding Molecule. DART-C is an Fc Domain-containing, bispecific diabody capable of binding CD16 and the HER2/neu Cancer Antigen. DART-C is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of humanized anti-human CD16 antibody hCD16-M1 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of Trastuzumab (and is thus immunospecific for an epitope of the HER2/neu Cancer Antigen). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HER2/neu Cancer Antigen (see, e.g.,  FIG. 4A ). 
     The first polypeptide chain of DART-C has the amino acid sequence of SEQ ID NO:156: 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITCRASQDVN TAVAWYQQKP 
               
               
                   
               
               
                   
                 GKAPKLLIYS ASFLYSGVPS RFSGSRSGTD FTLTISSLQP 
               
               
                   
               
               
                   
                 EDFATYYCQQ HYTTPPTFGQ GTKVEIK     EVQLV 
               
               
                   
               
               
                   
                 ESGGGLVKPG GSLRLSCAAS GFTFSNYGMS WVRQAPGKGL 
               
               
                   
               
               
                   
                 EWVATISGGG SYTFYPDSVK GRFTISRDNA KNSLYLQMNS 
               
               
                   
               
               
                   
                 LRTEDTALYY CVRQSARAPE PYWGQGTLVT VSS   ASTKG   EV 
               
               
                   
               
               
                   
                 AACEKEVAAL EKEVAALEKE VAALEKGGGD KTHTCPPCPA 
               
               
                   
               
               
                   
                 PEAAGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP 
               
               
                   
               
               
                   
                 EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ 
               
               
                   
               
               
                   
                 DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL 
               
               
                   
               
               
                   
                 PPSREEMTKN QVSLWCLVKG FYPSDIAVEW ESNGQPENNY 
               
               
                   
               
               
                   
                 KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA 
               
               
                   
               
               
                   
                 LHNHYTQKSL SLSPGK 
               
            
           
         
       
     
     Residues 1-107 of the first polypeptide chain (SEQ ID NO:156) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Trastuzumab (SEQ ID NO:125). Residues 108-115 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-233 of the first polypeptide chain correspond to the VH Domain of the humanized anti-human CD16 antibody hCD16-M1 (SEQ ID NO:72). Residues 234-238 correspond to a linker (SEQ ID NO:21, underlined). Residues 239-266 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 267-279 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 280-496 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of DART-C has the amino acid sequence of SEQ ID NO:157: 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSF LSASVGDRVT ITCRASQNVG THVAWYQQKP 
               
               
                   
               
               
                   
                 GKAPKSLLYS ASYRYSGVPS RFSGSGSGTD FTLTISSLQS 
               
               
                   
               
               
                   
                 EDIATYYCQQ YKSYPLTFGQ GTKLEIK     EVQLV 
               
               
                   
               
               
                   
                 ESGGGLVQPG GSLRLSCAAS GFNIKDTYIH WVRQAPGKGL 
               
               
                   
               
               
                   
                 EWVARIYPTN GYTRYADSVK GRFTISADTS KNTAYLQMNS 
               
               
                   
               
               
                   
                 LRAEDTAVYY CSRWGGDGFY AMDYWGQGTL VTVSS   ASTKG     
               
               
                   
               
               
                   
                 KVAACKEKVA ALKEKVAALK EKVAALKE 
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:157) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of humanized anti-human CD16 antibody hCD16-M1 (SEQ ID NO:73). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-235 of the second polypeptide chain correspond to the VH Domain of Trastuzumab (SEQ ID NO:124). Residues 236-240 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 241-268 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of DART-C has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     D. CD16 x HER2/neu Binding Molecule “DART-D” 
     The CD16 x HER2/neu Binding Molecule designated “DART-D” is a further illustrative CD16 x DA Binding Molecule. DART-D is an Fc Domain-containing, bispecific diabody capable of binding CD16 and the HER2/neu Cancer Antigen. DART-D is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of humanized anti-human CD16 antibody hCD16-M2 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of Trastuzumab (and is thus immunospecific for an epitope of the HER2/neu Cancer Antigen). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HER2/neu Cancer Antigen (see, e.g.,  FIG. 4A ). 
     The first polypeptide chain of DART-D has the amino acid sequence of SEQ ID NO:158: 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITCRASQDVN TAVAWYQQKP 
               
               
                   
               
               
                   
                 GKAPKLLIYS ASFLYSGVPS RFSGSRSGTD FTLTISSLQP 
               
               
                   
               
               
                   
                 EDFATYYCQQ HYTTPPTFGQ GTKVEIK     QVQLV 
               
               
                   
               
               
                   
                 QSGAEVKKPG ASVKVSCKAS GYTFTSSAMH WVRQAPGQGL 
               
               
                   
               
               
                   
                 EWMGYINHYN DGIKYNERFK GRVTITADKS TSTAYMELSS 
               
               
                   
               
               
                   
                 LRSEDTAVYY CATGYRYASW FASWGQGTLV TVSS   ASTKG   E 
               
               
                   
               
               
                   
                 VAACEKEVAA LEKEVAALEK EVAALEKGGG DKTHTCPPCP 
               
               
                   
               
               
                   
                 APEAAGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED 
               
               
                   
               
               
                   
                 PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 
               
               
                   
               
               
                   
                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 
               
               
                   
               
               
                   
                 LPPSREEMTK NQVSLWCLVK GFYPSDIAVE WESNGQPENN 
               
               
                   
               
               
                   
                 YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE 
               
               
                   
               
               
                   
                 ALHNHYTQKS LSLSPGK 
               
            
           
         
       
     
     Residues 1-107 of the first polypeptide chain (SEQ ID NO:158) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Trastuzumab (SEQ ID NO:125). Residues 108-115 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-234 of the first polypeptide chain correspond to the VH1 Domain of the humanized anti-human CD16 antibody hCD16-M2 (SEQ ID NO:83). Residues 235-239 correspond to a linker (SEQ ID NO:21, underlined). Residues 240-267 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 268-280 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 281-497 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of DART-D has the amino acid sequence of SEQ ID NO:159: 
     
       
         
           
               
               
            
               
                   
                 EIVLTQSPAT LSVSPGERAT LSCRASQNIG TSIHWYQQKP 
               
               
                   
               
               
                   
                 DQSPKLLIKS VSESISGVPS RFSGSGSGTD FTLTINSLEA 
               
               
                   
               
               
                   
                 EDFATYYCQQ SNSWPLTFGQ GTKLEIK     EVQLV 
               
               
                   
               
               
                   
                 ESGGGLVQPG GSLRLSCAAS GFNIKDTYIH WVRQAPGKGL 
               
               
                   
               
               
                   
                 EWVARIYPTN GYTRYADSVK GRFTISADTS KNTAYLQMNS 
               
               
                   
               
               
                   
                 LRAEDTAVYY CSRWGGDGFY AMDYWGQGTL VTVSS   ASTKG     
               
               
                   
               
               
                   
                 KVAACKEKVA ALKEKVAALK EKVAALKE 
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:159) of such illustrative CD16 x DA Binding Molecule correspond to the VL1 Domain of humanized anti-human CD16 antibody hCD16-M2 (SEQ ID NO:85). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-235 of the second polypeptide chain correspond to the VH Domain of Trastuzumab (SEQ ID NO:124). Residues 236-240 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 241-268 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of DART-D has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     E. CD16 x HER2/Neu Binding Molecule “DART-E” 
     The CD16 x HER2/neu Binding Molecule designated “DART-E” is a further illustrative CD16 x DA Binding Molecule. DART-E is a bispecific diabody capable of binding CD16 and the HER2/neu Cancer Antigen. DART-E is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of humanized anti-human CD16 antibody hCD16-M2 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of Trastuzumab (and is thus immunospecific for an epitope of the HER2/neu Cancer Antigen). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HER2/neu Cancer Antigen (see, e.g.,  FIG. 4A ). 
     The first polypeptide chain of DART-E has the amino acid sequence of SEQ ID NO:160: 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSS LSASVGDRVT ITCRASQDVN TAVAWYQQKP 
               
               
                   
               
               
                   
                 GKAPKLLIYS ASFLYSGVPS RFSGSRSGTD FTLTISSLQP 
               
               
                   
               
               
                   
                 EDFATYYCQQ HYTTPPTFGQ GTKVEIK     QVQLV 
               
               
                   
               
               
                   
                 QSGAEVKKPG ASVKVSCKAS GYTFTSSAMH WVRQAPGQGL 
               
               
                   
               
               
                   
                 EWMGYINHYN DGIKYNERFK GRVTITADKS TSTAYMELSS 
               
               
                   
               
               
                   
                 LRSEDTAVYY CARGYRYASW FASWGQGTLV TVSS   ASTKG   E 
               
               
                   
               
               
                   
                 VAACEKEVAA LEKEVAALEK EVAALEKGGG DKTHTCPPCP 
               
               
                   
               
               
                   
                 APEAAGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED 
               
               
                   
               
               
                   
                 PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 
               
               
                   
               
               
                   
                 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 
               
               
                   
               
               
                   
                 LPPSREEMTK NQVSLWCLVK GFYPSDIAVE WESNGQPENN 
               
               
                   
               
               
                   
                 YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE 
               
               
                   
               
               
                   
                 ALHNHYTQKS LSLSPGK 
               
            
           
         
       
     
     Residues 1-107 of the first polypeptide chain (SEQ ID NO:160) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Trastuzumab (SEQ ID NO:125). Residues 108-115 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-234 of the first polypeptide chain correspond to the VH2 Domain of the humanized anti-human CD16 antibody hCD16-M2 (SEQ ID NO:84). Residues 235-239 correspond to a linker (SEQ ID NO:21, underlined). Residues 240-267 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 268-280 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 281-497 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of DART-E is identical in sequence to the second polypeptide chain of DART-D (SEQ ID NO:159). 
     The third polypeptide chain of DART-E has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     F. CD16 x HIV Env Binding Molecule “DART-F” 
     The CD16 x HIV env Binding Molecule designated “DART-F” is a further illustrative CD16 x DA Binding Molecule. DART-F is a bispecific diabody capable of binding CD16 and the HIV env protein. DART-F is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of humanized anti-human CD16 antibody hCD16-M1 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of Antibody A32 (and is thus immunospecific for an epitope of the HIV env protein). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HIV env protein (see, e.g.,  FIG. 4A ). 
     The first polypeptide chain of DART-F has the amino acid sequence of SEQ ID NO:161: 
     
       
         
           
               
               
            
               
                 QSALTQPPSA SGSPGQSVTI SCTGTSSDVG GYNYVSWYQH HPGKAPKLII  
                   
               
               
                   
               
               
                 SEVNNRPSGV PDRFSGSKSG NTASLTVSGL QAEDEAEYYC SSYTDIHNFV  
               
               
                   
               
               
                 FGGGTKLTVL    EV QLVESGGGLV KPGGSLRLSC AASGFTFSNY  
               
               
                   
               
               
                 GMSWVRQAPG KGLEWVATIS GGGSYTFYPD SVKGRFTISR DNAKNSLYLQ  
               
               
                   
               
               
                 MNSLRTEDTA LYYCVRQSAR APEPYWGQGT LVTVSS   ASTK       G   EVAACEKEV  
               
               
                   
               
               
                 AALEKEVAAL EKEVAALEKG GGDKTHTCPP CPAPEAAGGP SVFLFPPKPK  
               
               
                   
               
               
                 DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS  
               
               
                   
               
               
                 TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV  
               
               
                   
               
               
                 YTLPPSREEM TKNQVSLWCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL  
               
               
                   
               
               
                 DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK  
               
            
           
         
       
     
     Residues 1-110 of the first polypeptide chain (SEQ ID NO:161) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Antibody A32 (SEQ ID NO:143). Residues 111-118 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 119-236 of the first polypeptide chain correspond to the VH Domain of the humanized anti-human CD16 antibody hCD16-M1 (SEQ ID NO:72). Residues 237-241 correspond to a linker (SEQ ID NO:21, underlined). Residues 242-269 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 270-282 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 283-499 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of DART-F has the amino acid sequence of SEQ ID NO:162: 
     
       
         
           
               
               
            
               
                 DIQMTQSPSF LSASVGDRVT ITCRASQNVG THVAWYQQKP GKAPKSLLYS  
                   
               
               
                   
               
               
                 ASYRYSGVPS RFSGSGSGTD FTLTISSLQS EDIATYYCQQ YKSYPLTFGQ  
               
               
                   
               
               
                 GTKLEIK     VQLQ ESGPGLVKPS QTLSLSCTVS GGSSSSGAHY  
               
               
                   
               
               
                 WSWIRQYPGK GLEWIGYIHY SGNTYYNPSL KSRITISQHT SENQFSLKLN  
               
               
                   
               
               
                 SVTVADTAVY YCARGTRLRT LRNAFDIWGQ GTLVTVSS   AS       TKG   KVAACKE  
               
               
                   
               
               
                 KVAALKEKVA ALKEKVAALK E  
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:162) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of humanized anti-human CD16 antibody hCD16-M1 (SEQ ID NO:73). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-238 of the second polypeptide chain correspond to the VH Domain of Antibody A32 (SEQ ID NO:142). Residues 239-243 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 244-271 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of DART-F has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     G. CD16 x HIV Env Binding Molecule “DART-G” 
     The CD16 x HIV env Binding Molecule designated “DART-G” is a further illustrative CD16 x DA Binding Molecule. DART-G is a bispecific diabody capable of binding CD16 and the HIV env protein. DART-G is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of humanized anti-human CD16 antibody hCD16-M2 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of Antibody A32 (and is thus immunospecific for an epitope of the HIV env protein). The three polypeptide chains associate to form a covalently bonded DART® diabody composed capable of immunospecifically binding the epitope of CD16 and the epitope of the HIV env protein (see, e.g.,  FIG. 4A ). 
     The first polypeptide chain of the DART-G has the amino acid sequence of SEQ ID NO:163: 
     
       
         
           
               
               
            
               
                 QSALTQPPSA SGSPGQSVTI SCTGTSSDVG GYNYVSWYQH HPGKAPKLII  
                   
               
               
                   
               
               
                 SEVNNRPSGV PDRFSGSKSG NTASLTVSGL QAEDEAEYYC SSYTDIHNFV  
               
               
                   
               
               
                 FGGGTKLTVL   QV QLVQSGAEVK KPGASVKVSC KASGYTFTSS  
               
               
                   
               
               
                 AMHWVRQAPG QGLEWMGYIN HYNDGIKYNE RFKGRVTITA DKSTSTAYME  
               
               
                   
               
               
                 LSSLRSEDTA VYYCATGYRY ASWFASWGQG TLVTVSS   AST       KG   EVAACEKE  
               
               
                   
               
               
                 VAALEKEVAA LEKEVAALEK GGGDKTHTCP PCPAPEAAGG PSVFLFPPKP  
               
               
                   
               
               
                 KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN  
               
               
                   
               
               
                 STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ  
               
               
                   
               
               
                 VYTLPPSREE MTKNQVSLWC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV  
               
               
                   
               
               
                 LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK  
               
            
           
         
       
     
     Residues 1-110 of the first polypeptide chain (SEQ ID NO:163) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Antibody A32 (SEQ ID NO:143). Residues 111-118 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 119-237 of the first polypeptide chain correspond to the VH Domain of the humanized anti-human CD16 antibody hCD16-M2 (SEQ ID NO:83). Residues 238-242 correspond to a linker (SEQ ID NO:21, underlined). Residues 243-270 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 271-283 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 284-500 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of the DART-G has the amino acid sequence of SEQ ID NO:164: 
     
       
         
           
               
               
            
               
                 EIVLTQSPAT LSVSPGERAT LSCRASQNIG TSIHWYQQKP DQSPKLLIKS  
                   
               
               
                   
               
               
                 VSESISGVPS RFSGSGSGTD FTLTINSLEA EDFATYYCQQ SNSWPLTFGQ  
               
               
                   
               
               
                 GTKLEIK     QVQLQ ESGPGLVKPS QTLSLSCTVS GGSSSSGAHY  
               
               
                   
               
               
                 WSWIRQYPGK GLEWIGYIHY SGNTYYNPSL KSRITISQHT SENQFSLKLN  
               
               
                   
               
               
                 SVTVADTAVY YCARGTRLRT LRNAFDIWGQ GTLVTVSS   AS       TKG   KVAACKE  
               
               
                   
               
               
                 KVAALKEKVA ALKEKVAALK E  
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:164) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of humanized anti-human CD16 antibody hCD16-M2 (SEQ ID NO:85). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-238 of the second polypeptide chain correspond to the VH Domain of Antibody A32 (SEQ ID NO:142). Residues 239-243 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 244-271 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of DART-G has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     H. CD16 x HIV Env Binding Molecule “DART-H” 
     The CD16 x HIV env Binding Molecule designated “DART-H” is a further illustrative CD16 x DA Binding Molecule. DART-H is a bispecific diabody capable of binding CD16 and the HIV env protein. DART-H is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of humanized anti-human CD16 antibody hCD16-M1 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of Antibody 7B2 (and is thus immunospecific for an epitope of the HIV env protein). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HIV env protein (see, e.g.,  FIG. 4A ). 
     The first polypeptide chain of DART-H has the amino acid sequence of SEQ ID NO:165: 
     
       
         
           
               
               
            
               
                 DIVMTQSPDS LAVSPGERAT IHCKSSQTLL YSSNNRHSIA WYQQRPGQPP  
                   
               
               
                   
               
               
                 KLLLYWASMR LSGVPDRFSG SGSGTDFTLT INNLQAEDVA IYYCHQYSSH  
               
               
                   
               
               
                 PPTFGHGTRV EIK     EVQLVESGG GLVKPGGSLR LSCAASGFTF  
               
               
                   
               
               
                 SNYGMSWVRQ APGKGLEWVA TISGGGSYTF YPDSVKGRFT ISRDNAKNSL  
               
               
                   
               
               
                 YLQMNSLRTE DTALYYCVRQ SARAPEPYWG QGTLVTVSS   A       STKG   EVAACE  
               
               
                   
               
               
                 KEVAALEKEV AALEKEVAAL EKGGGDKTHT CPPCPAPEAA GGPSVFLFPP  
               
               
                   
               
               
                 KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ  
               
               
                   
               
               
                 YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE  
               
               
                   
               
               
                 PQVYTLPPSR EEMTKNQVSL WCLVKGFYPS DIAVEWESNG QPENNYKTTP  
               
               
                   
               
               
                 PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP  
               
               
                 GK  
               
            
           
         
       
     
     Residues 1-113 of the first polypeptide chain (SEQ ID NO:165) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Antibody 7B2 (SEQ ID NO:141). Residues 114-121 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 122-239 of the first polypeptide chain correspond to the VH Domain of the humanized anti-human CD16 antibody hCD16-M1 (SEQ ID NO:72). Residues 240-244 correspond to a linker (SEQ ID NO:21, underlined). Residues 245-272 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 273-285 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 286-502 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of DART-H has the amino acid sequence of SEQ ID NO:166: 
     
       
         
           
               
               
            
               
                 DIQMTQSPSF LSASVGDRVT ITCRASQNVG THVAWYQQKP GKAPKSLLYS  
                   
               
               
                   
               
               
                 ASYRYSGVPS RFSGSGSGTD FTLTISSLQS EDIATYYCQQ YKSYPLTFGQ  
               
               
                   
               
               
                 GTKLEIK     QVQLV QSGGGVFKPG GSLRLSCEAS GFTFTEYYMT  
               
               
                   
               
               
                 WVRQAPGKGL EWLAYISKNG EYSKYSPSSN GRFTISRDNA KNSVFLQLDR  
               
               
                   
               
               
                 LSADDTAVYY CARADGLTYF SELLQYIFDL WGQGARVTVS S   ASTKG   KVAA  
               
               
                   
               
               
                 CKEKVAALKE KVAALKEKVA ALKE  
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:166) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of humanized anti-human CD16 antibody hCD16-M1 (SEQ ID NO:73). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-241 of the second polypeptide chain correspond to the VH Domain of Antibody 7B2 (SEQ ID NO:140). Residues 242-246 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 247-274 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of DART-H has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     I. CD16 x HER2/Neu Binding Molecule “DART-I” 
     The CD16 x HER2/neu Binding Molecule designated “DART-I” is a further illustrative CD16 x DA Binding Molecule. DART-I is similar to the above-described DART-C, but contains the VH of hCD16-M1A (comprising a mutated CDR H 3). As indicated above, the VL Domain of hCD16-M1A has the same amino acid sequence as the VL Domain of hCD16-M1. 
     The first polypeptide chain of DART-I has the amino acid sequence of SEQ ID NO:186: 
     
       
         
           
               
               
            
               
                 DIQMTQSPSS LSASVGDRVT ITCRASQDVN TAVAWYQQKP GKAPKLLIYS  
                   
               
               
                   
               
               
                 ASFLYSGVPS RFSGSRSGTD FTLTISSLQP EDFATYYCQQ HYTTPPTFGQ  
               
               
                   
               
               
                 GTKVEIK     EVQLV ESGGGLVKPG GSLRLSCAAS GFTFSNYGMS  
               
               
                   
               
               
                 WVRQAPGKGL EWVATISGGG SYTFYPDSVK GRFTISRDNA KNSLYLQMNS  
               
               
                   
               
               
                 LRTEDTALYY CVRQSANSPV PYWGQGTLVT VSS  EV AACEKEVAAL  
               
               
                   
               
               
                 EKEVAALEKE VAALEK GGGD KTHTCPPCP A PEAAGGPSVF LFPPKPKDTL  
               
               
                   
               
               
                 MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR  
               
               
                   
               
               
                 VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL  
               
               
                   
               
               
                 PPSREEMTKN QVSLWCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD  
               
               
                   
               
               
                 GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK  
               
            
           
         
       
     
     Residues 1-107 of the first polypeptide chain (SEQ ID NO:186) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Trastuzumab (SEQ ID NO:125). Residues 108-115 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-233 of the first polypeptide chain correspond to the VH Domain of the optimized anti-human CD16 antibody hCD16-M1A (SEQ ID NO:58). Residues 234-238 correspond to a linker (SEQ ID NO:21, underlined). Residues 239-266 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 267-279 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 280-496 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     Since the VL Domain of hCD16-M1A is the same as that of hCD16-M1, the amino acid sequence of the second polypeptide chain of DART-I is the same as that of the second polypeptide chain of the DART-C (i.e., SEQ ID NO:157). Similarly, the third polypeptide chain of DART-I has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     J. CD16 x HER2/Neu Binding Molecule “DART-J” 
     The CD16 x HER2/neu Binding Molecule designated “DART-J” is a further illustrative CD16 x DA Binding Molecule. DART-J is similar to the above-described DART-C, but contains the VL of hCD16-M1B (comprising a mutated CDR L 3). As indicated above, the VH Domain of hCD16-M1B has the same amino acid sequence as the VH Domain of hCD16-M1. 
     Since the VH Domain of hCD16-M1B is the same as that of hCD16-M1, the amino acid sequence of the first polypeptide chain of DART-J is the same as that of the first polypeptide chain of the DART-C (i.e., SEQ ID NO:156). 
     The second polypeptide chain of DART-J has the amino acid sequence of SEQ ID NO:187: 
     
       
         
           
               
               
            
               
                 DIQMTQSPSF LSASVGDRVT ITCRASQNVG THVAWYQQKP GKAPKSLLYS  
                   
               
               
                   
               
               
                 ASYRYSGVPS RFSGSGSGTD FTLTISSLQS EDIATYYCQD YTNYPLTFGQ  
               
               
                   
               
               
                 GTKLEIK   EVQLV ESGGGLVQPG GSLRLSCAAS GFNIKDTYIH  
               
               
                   
               
               
                 WVRQAPGKGL EWVARIYPTN GYTRYADSVK GRFTISADTS KNTAYLQMNS  
               
               
                   
               
               
                 LRAEDTAVYY CSRWGGDGFY AMDYWGQGTL VTVSS   ASTKG    KVAACKEKVA  
               
               
                   
               
               
                 ALKEKVAALK EKVAALKE  
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:157) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of humanized anti-human CD16 antibody hCD16-M1B (SEQ ID NO:59). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-235 of the second polypeptide chain correspond to the VH Domain of Trastuzumab (SEQ ID NO:124). Residues 236-240 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 241-268 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of DART-J has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     K. CD16 x HER2/Neu Binding Molecule “DART-K” 
     The CD16 x HER2/neu Binding Molecule designated “DART-K” is a further illustrative CD16 x DA Binding Molecule. DART-K is similar to the above-described DART-J, but also contains VH of hCD16-M1A (comprising a mutated CDR H 3) Thus, DART-K contains the VL and VH of hCD16-M1AB (comprising a mutated CDR L 3 and a mutated CDR H 3). 
     Since DART-K contains the VH of hCD16-M1A the amino acid sequence of the first polypeptide chain of DART-K is the same as that of the first polypeptide chain of the DART-I (i.e., SEQ ID NO:186). 
     Since DART-K contains VL Domain of hCD16-M1B, the amino acid sequence of the second polypeptide chain of DART-K is the same as that of the second polypeptide chain of the DART-J (i.e., SEQ ID NO:187). 
     The third polypeptide chain of DART-J has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     L. CD16 x CD19 Binding Molecule “DART-L” 
     The CD16 x CD19 Binding Molecule designated “DART-L” is another illustrative CD16 x DA Binding Molecule. DART-L is an Fc Domain-containing, bispecific diabody capable of binding CD16 and the CD19 B-cell tumor antigen. DART-L is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of anti-human CD16 antibody hCD16-M1 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of CD19 mAb 1 (and is thus immunospecific for an epitope of the CD19 Cancer Antigen). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the CD19 Cancer Antigen (see, e.g.,  FIG. 4A ). 
     The first polypeptide chain of DART-L has the amino acid sequence of SEQ ID NO:188: 
     
       
         
           
               
               
            
               
                 ENVLTQSPAT LSVTPGEKAT ITCRASQSVS YMHWYQQKPG QAPRLLIYDA  
                   
               
               
                   
               
               
                 SNRASGVPSR FSGSGSGTDH TLTISSLEAE DAATYYCFQG SVYPFTFGQG  
               
               
                   
               
               
                 TKLEIK     EVQLVE SGGGLVKPGG SLRLSCAASG FTFSNYGMSW  
               
               
                   
               
               
                 VRQAPGKGLE WVATISGGGS YTFYPDSVKG RFTISRDNAK NSLYLQMNSL  
               
               
                   
               
               
                 RTEDTALYYC VRQSARAPEP YWGQGTLVTV SS  EV AACEKEVAAL  
               
               
                   
               
               
                 EKEVAALEKE VAALEK GGGD   KTHTCPPCP A PEAAGGPSVF LFPPKPKDTL  
               
               
                   
               
               
                 MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR  
               
               
                   
               
               
                 VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL  
               
               
                   
               
               
                 PPSREEMTKN QVSLWCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD  
               
               
                   
               
               
                 GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK  
               
            
           
         
       
     
     Residues 1-106 of the first polypeptide chain (SEQ ID NO:188) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of CD19 mAb 1 (SEQ ID NO:139). Residues 107-114 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 115-232 of the first polypeptide chain correspond to the VH Domain of anti-human CD16 antibody hCD16-M1 (SEQ ID NO:72). Residues 233-238 correspond to a linker (SEQ ID NO:17, underlined). Residues 239-266 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 267-279 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 280-496 of the first polypeptide chain correspond to a “knob-bearing” (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of DART-L has the amino acid sequence of SEQ ID NO:189: 
     
       
         
           
               
               
            
               
                 DIQMTQSPSF LSASVGDRVT ITCRASQNVG THVAWYQQKP GKAPKSLLYS  
                   
               
               
                   
               
               
                 ASYRYSGVPS RFSGSGSGTD FTLTISSLQS EDIATYYCQQ YKSYPLTFGQ  
               
               
                   
               
               
                 GTKLEIK  QVTLR ESGPALVKPT QTLTLTCTFS GFSLSTSGMG  
               
               
                   
               
               
                 VGWIRQPPGK ALEWLAHIWW DDDKRYNPAL KSRLTISKDT SKNQVFLTMT  
               
               
                   
               
               
                 NMDPVDTATY YCARMELWSY YFDYWGQGTT VTVSS   GGCGG G   KVAACKEKV  
               
               
                   
               
               
                 AALKEKVAAL KEKVAALKE  
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:189) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of anti-human CD16 antibody hCD16-M1 (SEQ ID NO:73). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-235 of the second polypeptide chain correspond to the VH Domain of CD19 mAb 1 (SEQ ID NO:138). Residues 236-241 of the second polypeptide chain correspond to a linker (SEQ ID NO:17, underlined). Residues 242-269 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of DART-L has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     M. CD16 x CD19 Binding Molecule “DART-M” 
     The CD16 x CD19 Binding Molecule designated “DART-M” is a further illustrative CD16 x DA Binding Molecule. DART-M is similar to the above-described DART-L, but contains the VL of hCD16-M1B (comprising a mutated CDR L 3). As indicated above, the VH Domain of hCD16-M1B has the same amino acid sequence as the VH Domain of hCD16-M1. 
     Since the VH Domain of hCD16-M1B is the same as that of hCD16-M1, the amino acid sequence of the first polypeptide chain of DART-M is the same as that of the first polypeptide chain of the DART-L (i.e., SEQ ID NO:188). 
     The second polypeptide chain of DART-M has the amino acid sequence of SEQ ID NO:190: 
     
       
         
           
               
               
            
               
                 DIQMTQSPSF LSASVGDRVT ITCRASQNVG THVAWYQQKP GKAPKSLLYS  
                   
               
               
                   
               
               
                 ASYRYSGVPS RFSGSGSGTD FTLTISSLQS EDIATYYCQD YTNYPLTFGQ  
               
               
                   
               
               
                 GTKLEIK  QVTLR ESGPALVKPT QTLTLTCTFS GFSLSTSGMG  
               
               
                   
               
               
                 VGWIRQPPGK ALEWLAHIWW DDDKRYNPAL KSRLTISKDT SKNQVFLTMT  
               
               
                   
               
               
                 NMDPVDTATY YCARMELWSY YFDYWGQGTT VTVSS   GGCGG               G   KVAACKEKV  
               
               
                   
               
               
                 AALKEKVAAL KEKVAALKE  
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:190) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of anti-human CD16 antibody hCD16-M1B (SEQ ID NO:59). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-235 of the second polypeptide chain correspond to the VH Domain of CD19 mAb 1 (SEQ ID NO:138). Residues 236-241 of the second polypeptide chain correspond to a linker (SEQ ID NO:17, underlined). Residues 242-269 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of DART-M has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     N. CD16 x CD19 Binding Molecule “DART-N” 
     The CD16 x CD19 Binding Molecule designated “DART-N” is a further illustrative CD16 x DA Binding Molecule. DART-N is similar to the above-described DART-M, but also contains the VH of hCD16-M1A (comprising a mutated CDR H 3). Thus, DART-N contains the VL and VH of hCD16-M1AB (comprising a mutated CDR L 3 and a mutated CDR H 3)). 
     The first polypeptide chain of DART-N has the amino acid sequence of SEQ ID NO:191: 
     
       
         
           
               
               
            
               
                 ENVLTQSPAT LSVTPGEKAT ITCRASQSVS YMHWYQQKPG QAPRLLIYDA  
                   
               
               
                   
               
               
                 SNRASGVPSR FSGSGSGTDH TLTISSLEAE DAATYYCFQG SVYPFTFGQG  
               
               
                   
               
               
                 TKLEIK     EVQLVE SGGGLVKPGG SLRLSCAASG FTFSNYGMSW  
               
               
                   
               
               
                 VRQAPGKGLE WVATISGGGS YTFYPDSVKG RFTISRDNAK NSLYLQMNSL  
               
               
                   
               
               
                 RTEDTALYYC VRQSANSPVP YWGQGTLVTV SS   GGCGGG   EV AACEKEVAAL  
               
               
                   
               
               
                 EKEVAALEKE VAALEKGGGD KTHTCPPCPA PEAAGGPSVF LFPPKPKDTL  
               
               
                   
               
               
                 MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR  
               
               
                   
               
               
                 VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL  
               
               
                   
               
               
                 PPSREEMTKN QVSLWCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD  
               
               
                   
               
               
                 GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK  
               
            
           
         
       
     
     Residues 1-106 of the first polypeptide chain (SEQ ID NO:191) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of CD19 mAb 1 (SEQ ID NO:139). Residues 107-114 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 115-232 of the first polypeptide chain correspond to the VH Domain of anti-human CD16 antibody hCD16-M1A (SEQ ID NO:58). Residues 233-238 correspond to a linker (SEQ ID NO:17, underlined). Residues 239-266 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 267-279 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 280-496 of the first polypeptide chain correspond to a “knob-bearing” (SEQ ID NO:51), in which the final residue is lysine. 
     Since DART-N contains VL Domain of hCD16-M1B, the amino acid sequence of the second polypeptide chain of DART-N is the same as that of the second polypeptide chain of the DART-M (i.e., SEQ ID NO:190). 
     The third polypeptide chain of DART-N has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     O. CD16 x HER2/Neu Binding Molecule “DART-1” 
     The CD16 x HER2/neu Binding Molecule designated “DART-1” is a further illustrative CD16 x DA Binding Molecule. DART-1 is a bispecific diabody capable of binding CD16 and the HER2/neu Cancer Antigen. DART-1 is essentially the same as DART-A, except that it possesses the VL and VH Domains of the humanized anti-human CD16 antibody h3G8 (see, e.g., U.S. Patent Publication No. 2004/0010124; PCT Publication No. WO 2017/142928; Li, W. et al. (2016) “ Identification Of High - Affinity Anti - CD 16 A Allotype Independent Human Antibody Domains ,” Exp. Mol. Pathol. 101(2):281-289):
         VH Domain of humanized Anti-Human CD16 mAb h3G8 (SEQ ID NO:62) is shown below (CDR H  residues are shown underlined):       

     
       
         
           
               
               
            
               
                 QVTLRESGPA LVKPTQTLTL TCTFSGFSLS    TSGMGVG   WIR QPPGKALEWL  
                   
               
               
                   
               
               
                 A   HIWWDDDKR       YNPALKS   RLT ISKDTSKNQV VLTMTNMDPV DTATYYCAQ   I     
               
               
                   
               
               
                     NPAWFAY   WGQ GTLVTVSS  
               
            
           
         
       
         
         
           
             VL Domain of humanized CD16 mAb h3G8 (SEQ ID NO:63) is shown below (CDR L  residues are shown underlined): 
           
         
       
    
                        DIVMTQSPDS LAVSLGERAT INC   KASQSVD       FDGDSFMN   WY QQKPGQPPKL                    LIY   TTSNLES    GVPDRFSGSG SGTDFTLTIS SLQAEDVAVY YC   QQSNEDPY                       T   FGQGTKLEI K             
instead of the VH and VL Domains of anti-human CD16 antibody CD16-M1 (SEQ ID NO:64 and SEQ ID NO:65, respectively) that are present in the DART-A. The VH and VL Domains of h3G8 are used herein as a comparator CD16 binding site. DART-1 is composed of three polypeptide chains having two Binding Domains that comprises the VL and VH Domains of anti-human CD16 antibody CD16-M1 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of Trastuzumab (and is thus immunospecific for an epitope of the HER2/neu Cancer Antigen). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HER2/neu Cancer Antigen (see, e.g.,  FIG. 4A ).
 
     The first polypeptide chain of DART-1 has the amino acid sequence of SEQ ID NO:167: 
     
       
         
           
               
               
            
               
                 DIQMTQSPSS LSASVGDRVT ITCRASQDVN TAVAWYQQKP GKAPKLLIYS  
                   
               
               
                   
               
               
                 ASFLYSGVPS RFSGSRSGTD FTLTISSLQP EDFATYYCQQ HYTTPPTFGQ  
               
               
                   
               
               
                 GTKVEIK     QVTLR ESGPALVKPT QTLTLTCTFS GFSLSTSGMG  
               
               
                   
               
               
                 VGWIRQPPGK ALEWLAHIWW DDDKRYNPAL KSRLTISKDT SKNQVVLTMT  
               
               
                   
               
               
                 NMDPVDTATY YCAQINPAWF AYWGQGTLVT VSS   ASTKG   EV AACEKEVAAL  
               
               
                   
               
               
                 EKEVAALEKE VAALEKGGGD KTHTCPPCPA PEAAGGPSVF LFPPKPKDTL  
               
               
                   
               
               
                 MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR  
               
               
                   
               
               
                 VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL  
               
               
                   
               
               
                 PPSREEMTKN QVSLWCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD  
               
               
                   
               
               
                 GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK  
               
            
           
         
       
     
     Residues 1-107 of the first polypeptide chain (SEQ ID NO:167) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Trastuzumab (SEQ ID NO:125). Residues 108-115 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-233 of the first polypeptide chain correspond to the VH Domain of the humanized anti-human CD16 antibody h3G8 (SEQ ID NO:62). Residues 234-238 correspond to a linker (SEQ ID NO:21, underlined). Residues 239-266 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 267-279 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 280-496 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of DART-1 has the amino acid sequence of SEQ ID NO:168: 
     
       
         
           
               
               
            
               
                   
                 DIVMTQSPDS LAVSLGERAT INCKASQSVD FDGDSFMNWY 
               
               
                   
                   
               
               
                   
                 QQKPGQPPKL LIYTTSNLES GVPDRFSGSG SGTDFTLTIS 
               
               
                   
                   
               
               
                   
                 SLQAEDVAVY YCQQSNEDPY TFGQGTKLEI K  E 
               
               
                   
                   
               
               
                   
                 VQLVESGGGL VQPGGSLRLS CAASGFNIKD TYIHWVRQAP 
               
               
                   
                   
               
               
                   
                 GKGLEWVARI YPTNGYTRYA DSVKGRFTIS ADTSKNTAYL 
               
               
                   
                   
               
               
                   
                 QMNSLRAEDT AVYYCSRWGG DGFYAMDYWG QGTLVTVSS   A     
               
               
                   
                   
               
               
                   
                     STKG   KVAACK EKVAALKEKV AALKEKVAAL KE 
               
            
           
         
       
     
     Residues 1-111 of the second polypeptide chain (SEQ ID NO:168) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of humanized anti-human CD16 antibody h3G8 (SEQ ID NO:63). Residues 112-119 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 120-239 of the second polypeptide chain correspond to the VH Domain of Trastuzumab (SEQ ID NO:124). Residues 240-244 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 244-272 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of DART-1 has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     P. CD16 x RSV Binding Molecule “DART-2” 
     The CD16 x RSV Binding Molecule designated “DART-2” is a further illustrative CD16 x DA Binding Molecule. DART-2 is a bispecific diabody capable of binding CD16 and the RSV glycoprotein F. DART-2 is essentially the same as the DART-1, except that it possesses the VH and VL Domains of the anti-RSV glycoprotein F antibody vPalivizumab (SEQ ID NO:144 and SEQ ID NO:145, respectively) instead of the VH and VL Domains of Trastuzumab (SEQ ID NO:124 and SEQ ID NO:125, respectively) that are present in DART-1 Diabody. Thus, DART-2 is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of the humanized anti-human CD16 antibody h3G8 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of vPalivizumab (and is thus immunospecific for an epitope of the RSV glycoprotein F). The third polypeptide chain has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of RSV glycoprotein F (see, e.g.,  FIG. 4A ). As noted above, the VH and VL Domains of h3G8 are used herein as a comparator CD16 binding site. 
     Q. CD16 x RSV Binding Molecule “DART-3” 
     The CD16 x RSV Binding Molecule designated “DART-3” is a further illustrative CD16 x DA Binding Molecule. The CD16 x RSV DART-3 is a bispecific diabody capable of binding CD16 and the RSV glycoprotein F. DART-3 is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of the humanized anti-human CD16 antibody hCD16-M1 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of vPalivizumab (and is thus immunospecific for an epitope of the RSV glycoprotein F). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the RSV glycoprotein F (see, e.g.,  FIG. 4A ). 
     The first polypeptide chain of DART-3 has the amino acid sequence of SEQ ID NO:169: 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPST LSASVGDRVT ITCRASQSVG YMHWYQQKPG 
               
               
                   
                   
               
               
                   
                 KAPKLLIYDT SKLASGVPSR FSGSGSGTEF TLTISSLQPD 
               
               
                   
                   
               
               
                   
                 DFATYYCFQG SGYPFTFGGG TKLEIK     EVQLVE 
               
               
                   
                   
               
               
                   
                 SGGGLVKPGG SLRLSCAASG FTFSNYGMSW VRQAPGKGLE 
               
               
                   
                   
               
               
                   
                 WVATISGGGS YTFYPDSVKG RFTISRDNAK NSLYLQMNSL 
               
               
                   
                   
               
               
                   
                 RTEDTALYYC VRQSARAPEP YWGQGTLVTV SS   ASTKG   EVA 
               
               
                   
                   
               
               
                   
                 ACEKEVAALE KEVAALEKEV AALEKGGGDK THTCPPCPAP 
               
               
                   
                   
               
               
                   
                 EAAGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE 
               
               
                   
                   
               
               
                   
                 VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD 
               
               
                   
                   
               
               
                   
                 WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP 
               
               
                   
                   
               
               
                   
                 PSREEMTKNQ VSLWCLVKGF YPSDIAVEWE SNGQPENNYK 
               
               
                   
                   
               
               
                   
                 TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL 
               
               
                   
                   
               
               
                   
                 HNHYTQKSLS LSPGK 
               
            
           
         
       
     
     Residues 1-106 of the first polypeptide chain (SEQ ID NO:169) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of vPalivizumab (SEQ ID NO:145). Residues 107-114 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 115-232 of the first polypeptide chain correspond to the VH Domain of the humanized anti-human CD16 antibody hCD16-M1 (SEQ ID NO:72). Residues 233-237 correspond to a linker (SEQ ID NO:21, underlined). Residues 238-265 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 266-278 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 279-495 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of DART-3 has the amino acid sequence of SEQ ID NO:170: 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSF LSASVGDRVT ITCRASQNVG THVAWYQQKP 
               
               
                   
                   
               
               
                   
                 GKAPKSLLYS ASYRYSGVPS RFSGSGSGTD FTLTISSLQS 
               
               
                   
                   
               
               
                   
                 EDIATYYCQQ YKSYPLTFGQ GTKLEIK     QVTLR 
               
               
                   
                   
               
               
                   
                 ESGPALVKPT QTLTLTCTFS GFSLSTSGMS VGWIRQPPGK 
               
               
                   
                   
               
               
                   
                 ALEWLADIWW DDKKDYNPSL KSRLTISKDT SKNQVVLKVT 
               
               
                   
                   
               
               
                   
                 NMDPADTATY YCARSMITNW YFDVWGAGTT VTVSS   ASTKG     
               
               
                   
                   
               
               
                   
                 KVAACKEKVA ALKEKVAALK EKVAALKE 
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:170) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of humanized anti-human CD16 antibody hCD16-M1 (SEQ ID NO:73). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-235 of the second polypeptide chain correspond to the VH Domain of vPalivizumab (SEQ ID NO:144). Residues 236-240 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 241-268 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of the DART-3 has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). 
     R. CD16 x HIV Env Binding Molecule “DART-4 
     The CD16 x HIV env Binding Molecule designated “DART-4” is a further illustrative CD16 x DA Binding Molecule. DART-4 is a bispecific diabody capable of binding CD16 and the HIV env protein. DART-4 is essentially the same as DART-1, except that it possesses the VH and VL Domains of the anti-HIV env protein antibody 7B2 (SEQ ID NO:140 and SEQ ID NO:141, respectively) instead of the VH and VL Domains of Trastuzumab (SEQ ID NO:124 and SEQ ID NO:125, respectively) that are present in DART-1. Thus, DART-4 is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of the humanized anti-human CD16 antibody h3G8 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of 7B2 (and is thus immunospecific for an epitope of the HIV Env protein). The third polypeptide chain has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HIV env protein (see, e.g.,  FIG. 4A ). As noted above, the VH and VL Domains of h3G8 are used herein as a comparator CD16 binding site. 
     S. CD16 x RSV Binding Molecule “DART-5” 
     The CD16 x RSV Binding Molecule designated “DART-5” is a further illustrative CD16 x DA Binding Molecule. DART-5 is a bispecific diabody capable of binding CD16 and the RSV glycoprotein F. DART-5 is essentially the same as DART-L, except that it possesses the VL and VH Domains of the anti-RSV glycoprotein F antibody vPalivizumab (SEQ ID NO:144 and SEQ ID NO:145, respectively) instead of the VH and VL Domains of CD19 mAb 1 (SEQ ID NO:138 and SEQ ID NO:139, respectively) that are present in DART-L. Thus, DART-5 is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of the humanized anti-human CD16 antibody hCD16-M1 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of vPalivizumab (and is thus immunospecific for an epitope of the RSV glycoprotein F). The third polypeptide chain has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of RSV glycoprotein F (see, e.g.,  FIG. 4A ). 
     T. CD16 x RSV Binding Molecule “DART-6” 
     The CD16 x RSV Binding Molecule designated “DART-6” is a further illustrative CD16 x DA Binding Molecule. DART-6 is a bispecific diabody capable of binding CD16 and the RSV glycoprotein F. DART-6 is essentially the same as DART-M, except that it possesses the VL and VH Domains of the anti-RSV glycoprotein F antibody vPalivizumab (SEQ ID NO:144 and SEQ ID NO:145, respectively) instead of the VH and VL Domains of CD19 mAb 1 (SEQ ID NO:138 and SEQ ID NO:139, respectively) that are present in DART-M. Thus, DART-6 is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of the optimized anti-human CD16 antibody hCD16-M1B (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of vPalivizumab (and is thus immunospecific for an epitope of the RSV glycoprotein F). The third polypeptide chain has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of RSV glycoprotein F (see, e.g.,  FIG. 4A ). 
     U. CD16 x RSV Binding Molecule “DART-7” 
     The CD16 x RSV Binding Molecule designated “DART-7” is a further illustrative CD16 x DA Binding Molecule. DART-7 is a bispecific diabody capable of binding CD16 and the RSV glycoprotein F. DART-7 is essentially the same as DART-N, except that it possesses the VL and VH Domains of the anti-RSV glycoprotein F antibody vPalivizumab (SEQ ID NO:144 and SEQ ID NO:145, respectively) instead of the VH and VL Domains of CD19 mAb 1 (SEQ ID NO:138 and SEQ ID NO:139, respectively) that are present in DART-N. Thus, DART-7 is composed of three polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of the optimized anti-human CD16 antibody hCD16-M1AB (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of vPalivizumab (and is thus immunospecific for an epitope of the RSV glycoprotein F). The third polypeptide chain has the amino acid sequence of the Common Diabody Polypeptide Chain (SEQ ID NO:153). The three polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of RSV glycoprotein F (see, e.g.,  FIG. 4A ). 
     V. CD16 x HIV Env Molecule “DART-X” 
     The CD16 x HIV env Binding Molecule designated “DART-X” is a further illustrative CD16 x DA Binding Molecule. DART-X is a bispecific diabody capable of binding CD16 and the HIV env protein. DART-X is composed of two polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of the anti-human CD16 antibody CD16-M1 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of 7B2 (and is thus immunospecific for an epitope of the HIV env protein). The two polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HIV env protein (see, e.g.,  FIGS. 1A-1B ). 
     The first polypeptide chain of DART-X has the amino acid sequence of SEQ ID NO:171: 
     
       
         
           
               
               
            
               
                   
                 DIVMTQSPDS LAVSPGERAT IHCKSSQTLL YSSNNRHSIA 
               
               
                   
                   
               
               
                   
                 WYQQRPGQPP KLLLYWASMR LSGVPDRFSG SGSGTDFTLT 
               
               
                   
                   
               
               
                   
                 INNLQAEDVA IYYCHQYSSH PPTFGHGTRV EIK   
               
               
                   
                   
               
               
                   
                    EVKLVESGG TLVKPGGSLK LSCAASGFTF NNYGMSWVRQ 
               
               
                   
                   
               
               
                   
                 TPEKRLEWVA TISGGGSYTF YPDSVKGRFT ISRDNAKNSL 
               
               
                   
                   
               
               
                   
                 YLQMSSLRSE DTALYYCIRQ SARAPEPYWG QGTLVTVSS   A     
               
               
                   
                   
               
               
                   
                     STKG   EVAACE KEVAALEKEV AALEKEVAAL EK 
               
            
           
         
       
     
     Residues 1-113 of the first polypeptide chain (SEQ ID NO:171) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of 7B2 (SEQ ID NO:141). Residues 114-121 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 122-239 of the first polypeptide chain correspond to the VH Domain of the anti-human CD16 antibody CD16-M1 (SEQ ID NO:64). Residues 240-244 correspond to a linker (SEQ ID NO:21, underlined). Residues 245-272 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). 
     The second polypeptide chain of DART-X has the amino acid sequence of SEQ ID NO:172: 
     
       
         
           
               
               
            
               
                   
                 DIVMTQSQKF MSTSVGDRVS VTCKASQNVG THVAWYQQKS 
               
               
                   
                   
               
               
                   
                 GQSPKSLLYS ASYRYSGVPD RFSGSGSGTD FTLTISNVQS 
               
               
                   
                   
               
               
                   
                 EDLAEYFCQQ YKSYPLTFGA GTKLELK     QVQLV 
               
               
                   
                   
               
               
                   
                 QSGGGVFKPG GSLRLSCEAS GFTFTEYYMT WVRQAPGKGL 
               
               
                   
                   
               
               
                   
                 EWLAYISKNG EYSKYSPSSN GRFTISRDNA KNSVFLQLDR 
               
               
                   
                   
               
               
                   
                 LSADDTAVYY CARADGLTYF SELLQYIFDL WGQGARVTVS 
               
               
                   
                   
               
               
                   
                 S   ASTKG   KVAA CKEKVAALKE KVAALKEKVA ALKE 
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:172) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of anti-human CD16 antibody CD16-M1 (SEQ ID NO:65). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-241 of the second polypeptide chain correspond to the VH Domain of 7B2 (SEQ ID NO:140). Residues 242-246 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 247-274 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     W. CD16 x HIV Env Binding Molecule “DART-Y” 
     The CD16 x HIV env Binding Molecule designated “DART-Y” is a further illustrative CD16 x DA Binding Molecule. DART-Y is a bispecific diabody capable of binding CD16 and the HIV env protein. DART-Y is composed of two polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of the anti-human CD16 antibody CD16-M2 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of 7B2 (and is thus immunospecific for an epitope of the HIV env protein). The two polypeptide chains associate to form a covalently bonded DART® diabody capable of immunospecifically binding the epitope of CD16 and the epitope of the HIV env protein (see, e.g.,  FIG. 1B ). 
     The first polypeptide chain of DART-Y has the amino acid sequence of SEQ ID NO:173: 
     
       
         
           
               
               
            
               
                   
                 DIVMTQSPDS LAVSPGERAT IHCKSSQTLL YSSNNRHSIA 
               
               
                   
                   
               
               
                   
                 WYQQRPGQPP KLLLYWASMR LSGVPDRFSG SGSGTDFTLT 
               
               
                   
                   
               
               
                   
                 INNLQAEDVA IYYCHQYSSH PPTFGHGTRV EIK   
               
               
                   
                   
               
               
                   
                    EVQLQQSGP ELVKPGASVK MSCKASGYTF TSSAMHWVKK 
               
               
                   
                   
               
               
                   
                 NPGQGLEWIG YINHYNDGIK YNERFKGKAT LTSDKSSSTA 
               
               
                   
                   
               
               
                   
                 YMELSSLTSE DSAVYYCATG YRYASWFASW GQGTLVTVSS 
               
               
                   
                   
               
               
                   
                     ASTKG   EVAAC EKEVAALEKE VAALEKEVAA LEK 
               
            
           
         
       
     
     Residues 1-113 of the first polypeptide chain (SEQ ID NO:173) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of 7B2 (SEQ ID NO:141). Residues 114-121 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 122-240 of the first polypeptide chain correspond to the VH Domain of the anti-human CD16 antibody CD16-M2 (SEQ ID NO:75). Residues 241-245 correspond to a linker (SEQ ID NO:21, underlined). Residues 246-273 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). 
     The second polypeptide chain of DART-Y has the amino acid sequence of SEQ ID NO:174: 
     
       
         
           
               
               
            
               
                   
                 DILLTQSPAI LSVSPGERVS FSCRASQNIG TSIHWYQQRT 
               
               
                   
                   
               
               
                   
                 DGSPRLLIKS VSESISGIPS RFSGSGSGTD FTLTINGVES 
               
               
                   
                   
               
               
                   
                 GDISDYYCQQ SNSWPLTFGA GTKLELK     QVQLV 
               
               
                   
                   
               
               
                   
                 QSGGGVFKPG GSLRLSCEAS GFTFTEYYMT WVRQAPGKGL 
               
               
                   
                   
               
               
                   
                 EWLAYISKNG EYSKYSPSSN GRFTISRDNA KNSVFLQLDR 
               
               
                   
                   
               
               
                   
                 LSADDTAVYY CARADGLTYF SELLQYIFDL WGQGARVTVS 
               
               
                   
                   
               
               
                   
                 S   ASTKG   KVAA CKEKVAALKE KVAALKEKVA ALKE 
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:174) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of anti-human CD16 antibody CD16-M2 (SEQ ID NO:76). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-241 of the second polypeptide chain correspond to the VH Domain of 7B2 (SEQ ID NO:140). Residues 242-246 of the second polypeptide chain correspond to a linker (SEQ ID NO:21, underlined). Residues 247-274 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     X. CD16 x HIV Env Binding Molecule “DART-0” 
     The CD16 x HIV env Binding Molecule designated “DART-0 is a further illustrative CD16 x DA Binding Molecule. DART-0 is a bispecific diabody capable of binding CD16 and the HIV env protein. DART-0 is essentially the same as DART-X, except that it possesses the VH and VL Domains of the humanized anti-human CD16 antibody h3G8 (SEQ ID NO:62 and SEQ ID NO:63, respectively) instead of the VH and VL Domains of anti-human CD16 antibody CD16-M1 (SEQ ID NO:64 and SEQ ID NO:65, respectively) that are present in the DART-X. Thus, DART-0 is composed of two polypeptide chains having one Binding Domain that comprises the VL and VH Domains of the humanized anti-human CD16 antibody h3G8 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of 7B2 (and is thus immunospecific for an epitope of the HIV Env protein). These polypeptide chains associate to form a covalently bonded DART® diabody composed of two polypeptide chains that possesses one Binding Domain immunospecific for the epitope of CD16 and one Binding Domain immunospecific for the epitope of the HIV env protein (see, e.g.,  FIG. 1A-1B ). As noted above, the VH and VL Domains of h3G8 are used herein as a comparator CD16 binding site. 
     Y. CD16 x HIV Env Binding Molecule “DART-Z” 
     The CD16 x HIV env Binding Molecule designated “DART-Z” is a further illustrative CD16 x DA Binding Molecule. DART-Z is a bispecific diabody capable of binding CD16 and the HIV env protein. DART-Z is essentially the same as DART-X, except that it possesses the VH and VL Domains of the anti-HIV env antibody A32 (SEQ ID NOs:142 and 143, respectively) rather than the VH and VL Domains of the anti-HIV env antibody 7B2, and it possesses the VH and VL Domains of the anti-human CD16A scFv 4-LS21 (U.S. Patent Publn. No. 2015/0218275): 
     VH Domain of Anti-Human CD16A scFv 4-LS21 (SEQ ID NO:175): 
                            EEVQLVQSGA EVKKPGESLK VSCKASGYTF TSYYMHWVRQ                       APGQGLEWMG IINPSGGSTS YAQKFQGRVT MTRDTSTSTV                       YMELSSLRSE DTAVYYCARG SAYYYDFADY WGQGTLVTVS S            
VL Domain of Anti-Human CD16A scFv 4-LS21 (SEQ ID NO:176):
 
                            QPVLTQPSSV SVAPGQTATI SCGGHNIGSK NVHWYQQRPG                       QSPVLVIYQD NKRPSGIPER FSGSNSGNTA TLTISGTQAM                       DEADYYCQVW DNYSVLFGGG TKLTVL            
instead of the VH and VL Domains of anti-human CD16 antibody CD16-M1 (SEQ ID NO:64 and SEQ ID NO:65, respectively) that are present in DART-X. Thus, DART-Z is composed of two polypeptide chains having one Binding Domain that comprises the VL and VH Domains of the anti-human CD16A scFv 4-LS21 (and is thus immunospecific for an epitope of CD16) and one Binding Domain that comprises the VL and VH Domains of A32 (and is thus immunospecific for an epitope of the HIV Env protein). These polypeptide chains associate to form a covalently bonded DART® diabody composed of two polypeptide chains that possesses one Binding Domain immunospecific for the epitope of CD16 and one Binding Domain immunospecific for the epitope of the HIV env protein (see, e.g.,  FIG. 1A-1B ). The VH and VL Domains of 4-LS21 are used herein as a comparator CD16 binding sites.
 
     Z. CD16 x HIV Env x HIV Env Trivalent Molecules 
     The CD16 x DA Binding Molecules of the present invention are further illustrated by Fc Domain-containing, bispecific or trispecific, CD16 x HIV env x HIV env Trivalent Molecules that comprise two Binding Domains capable of binding to an epitope of the HIV env protein and one Binding Domain capable of binding to an epitope of CD16. Such epitopes of the HIV env protein may be the same (as in the CD16 x HIV env x HIV env Trivalent Molecule “TRIDENT-A”) or they may be different (as in the CD16 x HIV env x HIV env Trivalent Molecule “TRIDENT-B”). The structures and sequences of illustrative Trivalent TRIDENT™ Molecules are summarized in Table 13, and are described in detail below. 
     
       
         
           
               
               
               
               
               
               
               
               
               
             
               
                 TABLE 13 
               
               
                   
               
               
                 Trivalent 
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 Molecule 
                 Polypeptide 
                 SEQ ID 
                   
                   
                 SEQ ID 
                   
                   
                 SEQ ID 
               
               
                 Name 
                 Chain 
                 NO 
                 Antibody 
                 Domain 
                 NO 
                 Antibody 
                 Domain 
                 NO 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 TRIDENT-A 
                 1 
                 177 
                 A32 
                 VL 
                 143 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 178 
                 hCD16-M1 
                 VL 
                 73 
                 A32 
                 VH 
                 142 
               
               
                   
                 3 
                 179 
                 A32 
                 VH 
                 142 
               
               
                   
                 4 
                 180 
                 A32 
                 VL 
                 143 
               
               
                 TRIDENT-B 
                 1 
                 177 
                 A32 
                 VL 
                 143 
                 hCD16-M1 
                 VH 
                 72 
               
               
                   
                 2 
                 178 
                 hCD16-M1 
                 VL 
                 73 
                 A32 
                 VH 
                 142 
               
               
                   
                 3 
                 181 
                 7B2 
                 VH 
                 140 
               
               
                   
                 4 
                 182 
                 7B2 
                 VL 
                 141 
               
               
                   
               
            
           
         
       
     
     1. CD16 x HIV Env x HIV Env Trivalent Molecule “TRIDENT-A” 
     CD16 x HIV env x HIV env Trivalent Molecule designated “TRIDENT-A” is composed of four polypeptide chains and possesses one Binding Domain that comprises the VL and VH Domains of anti-human CD16 antibody hCD16-M1 (and is thus immunospecific for an epitope of CD16) and two Binding Domains that each comprise the VL and VH Domains of A32 (and is thus immunospecific for the epitope of the HIV env protein recognized by antibody A32). The four polypeptide chains associate to form a covalently bonded TRIDENT™ molecule capable of immunospecifically binding one or two copies of the HIV env protein epitope recognized by antibody A32, and an epitope of CD16 (see  FIG. 6A ). 
     The first polypeptide chain of TRIDENT-A has the amino acid sequence of SEQ ID NO:177: 
     
       
         
           
               
               
            
               
                   
                 QSALTQPPSA SGSPGQSVTI SCTGTSSDVG GYNYVSWYQH 
               
               
                   
                   
               
               
                   
                 HPGKAPKLII SEVNNRPSGV PDRFSGSKSG NTASLTVSGL 
               
               
                   
                   
               
               
                   
                 QAEDEAEYYC SSYTDIHNFV FGGGTKLTVL   EV 
               
               
                   
                   
               
               
                   
                 QLVESGGGLV KPGGSLRLSC AASGFTFSNY GMSWVRQAPG 
               
               
                   
                   
               
               
                   
                 KGLEWVATIS GGGSYTFYPD SVKGRFTISR DNAKNSLYLQ 
               
               
                   
                   
               
               
                   
                 MNSLRTEDTA LYYCVRQSAR APEPYWGQGT LVTVSS   ASTK     
               
               
                   
                   
               
               
                   
                     G   EVAACEKEV AALEKEVAAL EKEVAALEKG GGDKTHTCPP 
               
               
                   
                   
               
               
                   
                 CPAPEAAGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH 
               
               
                   
                   
               
               
                   
                 EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV 
               
               
                   
                   
               
               
                   
                 LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV 
               
               
                   
                   
               
               
                   
                 YTLPPSREEM TKNQVSLWCL VKGFYPSDIA VEWESNGQPE 
               
               
                   
                   
               
               
                   
                 NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM 
               
               
                   
                   
               
               
                   
                 HEALHNHYTQ KSLSLSPGK 
               
            
           
         
       
     
     Residues 1-110 of the first polypeptide chain (SEQ ID NO:177) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Antibody A32 (SEQ ID NO:143). Residues 111-118 (double underlined) of the first polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 119-236 of the first polypeptide chain correspond to the VH Domain of anti-human CD16 antibody hCD16-M1 (SEQ ID NO:72). Residues 237-241 correspond to a linker (SEQ ID NO:21, underlined). Residues 242-269 of the first polypeptide chain correspond to a cysteine-containing E-coil (SEQ ID NO:31). Residues 270-282 of the first polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 283-499 of the first polypeptide chain correspond to a “knob-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:51), in which the final residue is lysine. 
     The second polypeptide chain of TRIDENT-A has the amino acid sequence of SEQ ID NO:178: 
     
       
         
           
               
               
            
               
                   
                 DIQMTQSPSF LSASVGDRVT ITCRASQNVG THVAWYQQKP 
               
               
                   
                   
               
               
                   
                 GKAPKSLLYS ASYRYSGVPS RFSGSGSGTD FTLTISSLQS 
               
               
                   
                   
               
               
                   
                 EDIATYYCQQ YKSYPLTFGQ GTKLEIK     QVQLQ 
               
               
                   
                   
               
               
                   
                 ESGPGLVKPS QTLSLSCTVS GGSSSSGAHY WSWIRQYPGK 
               
               
                   
                   
               
               
                   
                 GLEWIGYIHY SGNTYYNPSL KSRITISQHT SENQFSLKLN 
               
               
                   
                   
               
               
                   
                 SVTVADTAVY YCARGTRLRT LRNAFDIWGQ GTLVTVSS   AS     
               
               
                   
                   
               
               
                   
                     TKG   KVAACKE KVAALKEKVA ALKEKVAALK E 
               
            
           
         
       
     
     Residues 1-107 of the second polypeptide chain (SEQ ID NO:178) of such illustrative CD16 x DA Binding Molecule correspond to the VL Domain of Antibody hCD16-M1 (SEQ ID NO:73). Residues 108-115 (double underlined) of the second polypeptide chain correspond to Linker 1 (GGGSGGGG; SEQ ID NO:16). Residues 116-238 of the second polypeptide chain correspond to the VH Domain of Antibody A32 (SEQ ID NO:142). Residues 239-243 of the second polypeptide chain correspond to a linker (SEQ ID NO:43). Residues 244-271 of the second polypeptide chain correspond to a cysteine-containing K-coil (SEQ ID NO:32). 
     The third polypeptide chain of TRIDENT-A has the amino acid sequence of SEQ ID NO:179: 
     
       
         
           
               
               
            
               
                   
                 QVQLQESGPG LVKPSQTLSL SCTVSGGSSS SGAHYWSWIR 
               
               
                   
                   
               
               
                   
                 QYPGKGLEWI GYIHYSGNTY YNPSLKSRIT ISQHTSENQF 
               
               
                   
                   
               
               
                   
                 SLKLNSVTVA DTAVYYCARG TRLRTLRNAF DIWGQGTLVT 
               
               
                   
                   
               
               
                   
                 VSSASTKGPS VFPLAPSSKS TSGGTAALGC LVKDYFPEPV 
               
               
                   
                   
               
               
                   
                 TVSWNSGALT SGVHTFPAVL QSSGLYSLSS VVTVPSSSLG 
               
               
                   
                   
               
               
                   
                 TQTYICNVNH KPSNTKVDKR V   EPKSCDKTH       TCPPCP   APEA 
               
               
                   
                   
               
               
                   
                 AGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK 
               
               
                   
                   
               
               
                   
                 FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL 
               
               
                   
                   
               
               
                   
                 NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS 
               
               
                   
                   
               
               
                   
                 REEMTKNQVS LSCAVKGFYP SDIAVEWESN GQPENNYKTT 
               
               
                   
                   
               
               
                   
                 PPVLDSDGSF FLVSKLTVDK SRWQQGNVFS CSVMHEALHN 
               
               
                   
                   
               
               
                   
                 RYTQKSLSLS PGK 
               
            
           
         
       
     
     Residues 1-123 of the third polypeptide chain (SEQ ID NO:179) correspond to the VH Domain of Antibody A32 (SEQ ID NO:142). Residues 124-221 of the third polypeptide chain correspond to a human IgG1 CH1 Domain (SEQ ID NO:3). Residues 222-236 of the third polypeptide chain correspond to an IgG Hinge Domain (SEQ ID NO:7, underlined). Residues 237-453 of the third polypeptide chain correspond to a “hole-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:53), in which the final residue is lysine. 
     The fourth polypeptide chain of TRIDENT-A has the amino acid sequence of SEQ ID NO:180: 
     
       
         
           
               
               
            
               
                   
                 QSALTQPPSA SGSPGQSVTI SCTGTSSDVG GYNYVSWYQH 
               
               
                   
                   
               
               
                   
                 HPGKAPKLII SEVNNRPSGV PDRFSGSKSG NTASLTVSGL 
               
               
                   
                   
               
               
                   
                 QAEDEAEYYC SSYTDIHNFV FGGGTKLTVL RTVAAPSVFI 
               
               
                   
                   
               
               
                   
                 FPPSDEQLKS GTASVVCLLN NFYPREAKVQ WKVDNALQSG 
               
               
                   
                   
               
               
                   
                 NSQESVTEQD SKDSTYSLSS TLTLSKADYE KHKVYACEVT 
               
               
                   
                   
               
               
                   
                 HQGLSSPVTK SFNRGEC 
               
            
           
         
       
     
     Residues 1-110 of the fourth polypeptide chain (SEQ ID NO:180) correspond to the VL Domain of Antibody A32 (SEQ ID NO:143). Residues 111-217 of the fourth polypeptide chain correspond to a human CL Kappa Domain (SEQ ID NO:1). 
     2. CD16 x HIV Env x HIV Env Trivalent Molecule “TRIDENT-B” 
     The illustrative CD16 x HIV env x HIV env Trivalent Molecule designated “TRIDENT-B” is also composed of four polypeptide chains. It possesses one Binding Domain that comprises the VL and VH Domains of anti-human CD16 antibody hCD16-M1 (and is thus immunospecific for an epitope of CD16), a Binding Domain that comprises the VL and VH Domains of A32 (and is thus immunospecific for the epitope of the HIV env protein recognized by antibody A32) and a further Binding Domain that comprises the VL and VH Domains of 7B2 (and is thus immunospecific for the epitope of the HIV env protein recognized by antibody 7B2). The four polypeptide chains associate to form a covalently bonded TRIDENT™ molecule capable of immunospecifically binding the epitope of the HIV env protein recognized by antibody A32 and/or the epitope of the HIV env protein recognized by antibody 7B2, and an epitope of CD16 (see  FIG. 6A ). 
     The first polypeptide chain of TRIDENT-B is identical to the first polypeptide chain of TRIDENT-A (SEQ ID NO:177). 
     The second polypeptide chain of TRIDENT-B is identical to the second polypeptide chain of TRIDENT-A (SEQ ID NO:178). 
     The third polypeptide chain of TRIDENT-B has the amino acid sequence of SEQ ID NO:181: 
     
       
         
           
               
               
            
               
                   
                 QVQLVQSGGG VFKPGGSLRL SCEASGFTFT EYYMTWVRQA 
               
               
                   
                   
               
               
                   
                 PGKGLEWLAY ISKNGEYSKY SPSSNGRFTI SRDNAKNSVF 
               
               
                   
                   
               
               
                   
                 LQLDRLSADD TAVYYCARAD GLTYFSELLQ YIFDLWGQGA 
               
               
                   
                   
               
               
                   
                 RVTVSSASTK GPSVFPLAPS SKSTSGGTAA LGCLVKDYFP 
               
               
                   
                   
               
               
                   
                 EPVTVSWNSG ALTSGVHTFP AVLQSSGLYS LSSVVTVPSS 
               
               
                   
                   
               
               
                   
                 SLGTQTYICN VNHKPSNTKV DKRV   EPKSCD       KTHTCPPCP   A 
               
               
                   
                   
               
               
                   
                 PEAAGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP 
               
               
                   
                   
               
               
                   
                 EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ 
               
               
                   
                   
               
               
                   
                 DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL 
               
               
                   
                   
               
               
                   
                 PPSREEMTKN QVSLSCAVKG FYPSDIAVEW ESNGQPENNY 
               
               
                   
                   
               
               
                   
                 KTTPPVLDSD GSFFLVSKLT VDKSRWQQGN VFSCSVMHEA 
               
               
                   
                   
               
               
                   
                 LHNRYTQKSL SLSPGK 
               
            
           
         
       
     
     Residues 1-126 of the third polypeptide chain (SEQ ID NO:181) correspond to the VH Domain of Antibody 7B2 (SEQ ID NO:140). Residues 127-224 of the third polypeptide chain correspond to a human IgG1 CH1 Domain (SEQ ID NO:3). Residues 225-239 of the third polypeptide chain correspond to an IgG Hinge Domain (SEQ ID NO:7, underlined). Residues 240-456 of the third polypeptide chain correspond to a “hole-bearing” IgG1 CH2-CH3 Domain (SEQ ID NO:53), in which the final residue is lysine. 
     The fourth polypeptide chain of TRIDENT-B has the amino acid sequence of SEQ ID NO:182: 
     
       
         
           
               
               
            
               
                   
                 DIVMTQSPDS LAVSPGERAT IHCKSSQTLL YSSNNRHSIA 
               
               
                   
                   
               
               
                   
                 WYQQRPGQPP KLLLYWASMR LSGVPDRFSG SGSGTDFTLT 
               
               
                   
                   
               
               
                   
                 INNLQAEDVA IYYCHQYSSH PPTFGHGTRV EIKRTVAAPS 
               
               
                   
                   
               
               
                   
                 VFIFPPSDEQ LKSGTASVVC LLNNFYPREA KVQWKVDNAL 
               
               
                   
                   
               
               
                   
                 QSGNSQESVT EQDSKDSTYS LSSTLTLSKA DYEKHKVYAC 
               
               
                   
                   
               
               
                   
                 EVTHQGLSSP VTKSFNRGEC 
               
            
           
         
       
     
     Residues 1-113 of the fourth polypeptide chain (SEQ ID NO:182) correspond to the VL Domain of Antibody 7B2 (SEQ ID NO:141). Residues 114-220 of the fourth polypeptide chain correspond to a human CL Kappa Domain (SEQ ID NO:1). 
     VIII. Methods of Production 
     The molecules of the present invention are most preferably produced through the recombinant expression of nucleic acid molecules that encode such polypeptides, as is well-known in the art. 
     Polypeptides of the invention may be conveniently prepared using solid phase peptide synthesis (Merrifield, B. (1986) “ Solid Phase Synthesis ,” Science 232(4748):341-347; Houghten, R. A. (1985) “ General Method For The Rapid Solid - Phase Synthesis Of Large Numbers Of Peptides: Specificity Of Antigen Antibody Interaction At The Level Of Individual Amino Acids ,” Proc. Natl. Acad. Sci. (U.S.A.) 82(15):5131-5135; Ganesan, A. (2006) “ Solid - Phase Synthesis In The Twenty - First Century ,” Mini Rev. Med. Chem. 6(1):3-10). 
     Antibodies may be made recombinantly and expressed using any method known in the art. Antibodies may be made recombinantly by first isolating the antibodies made from host animals, obtaining the gene sequence, and using the gene sequence to express the antibody recombinantly in host cells (e.g., CHO cells). Another method that may be employed is to express the antibody sequence in plants (e.g., tobacco) or transgenic milk. Suitable methods for expressing antibodies recombinantly in plants or milk have been disclosed (see, for example, Peeters et al. (2001) “ Production Of Antibodies And Antibody Fragments In Plants ,” Vaccine 19:2756; Lonberg, N. et al. (1995) “ Human Antibodies From Transgenic Mice ,” Int. Rev. Immunol 13:65-93; and Pollock et al. (1999) “ Transgenic Milk As A Method For The Production Of Recombinant Antibodies ,” J. Immunol. Methods 231:147-157). Suitable methods for making derivatives of antibodies, e.g., humanized, single-chain, etc. are known in the art, and have been described above. In another alternative, antibodies may be made recombinantly by phage display technology (see, for example, U.S. Pat. Nos. 5,565,332; 5,580,717; 5,733,743; 6,265,150; and Winter, G. et al. (1994) “ Making Antibodies By Phage Display Technology ,” Annu. Rev. Immunol. 12.433-455). 
     Vectors containing polynucleotides of interest (e.g., polynucleotides encoding the polypeptide chains of the binding molecules of the present invention) can be introduced into the host cell by any of a number of appropriate means, including electroporation, transfection employing calcium chloride, rubidium chloride, calcium phosphate, DEAE-dextran, or other substances; microprojectile bombardment; lipofection; and infection (e.g., where the vector is an infectious agent such as vaccinia virus). The choice of introducing vectors or polynucleotides will often depend on features of the host cell. 
     Any host cell capable of overexpressing heterologous DNAs can be used for the purpose of expressing a polypeptide or protein of interest. Non-limiting examples of suitable mammalian host cells include but are not limited to COS, HeLa, and CHO cells. 
     The invention includes polypeptides comprising an amino acid sequence of a binding molecule of this invention. The polypeptides of this invention can be made by procedures known in the art. The polypeptides can be produced by proteolytic or other degradation of the antibodies, by recombinant methods (i.e., single or fusion polypeptides) as described above or by chemical synthesis. Polypeptides of the antibodies, especially shorter polypeptides up to about 50 amino acids, are conveniently made by chemical synthesis. Methods of chemical synthesis are known in the art and are commercially available. 
     The invention includes variants of the disclosed binding molecules, including functionally equivalent polypeptides that do not significantly affect the properties of such molecules as well as variants that have enhanced or decreased activity. Modification of polypeptides is routine practice in the art and need not be described in detail herein. Examples of modified polypeptides include polypeptides with conservative substitutions of amino acid residues, one or more deletions or additions of amino acids which do not significantly or deleteriously change the functional activity, or use of chemical analogs. Amino acid residues that can be conservatively substituted for one another include but are not limited to: glycine/alanine; serine/threonine; valine/isoleucine/leucine; asparagine/glutamine; aspartic acid/glutamic acid; lysine/arginine; and phenylalanine/tyrosine. These polypeptides also include glycosylated and non-glycosylated polypeptides, as well as polypeptides with other post-translational modifications, such as, for example, glycosylation with different sugars, acetylation, and phosphorylation. Preferably, the amino acid substitutions would be conservative, i.e., the substituted amino acid would possess similar chemical properties as that of the original amino acid. Such conservative substitutions are known in the art, and examples have been provided above. Amino acid modifications can range from changing or modifying one or more amino acids to complete redesign of a region, such as the Variable Domain. Changes in the Variable Domain can alter binding affinity and/or specificity. Other methods of modification include using coupling techniques known in the art, including, but not limited to, enzymatic means, oxidative substitution and chelation. Modifications can be used, for example, for attachment of labels for immunoassay, such as the attachment of radioactive moieties for radioimmunoassay. Modified polypeptides are made using established procedures in the art and can be screened using standard assays known in the art. 
     In one embodiment, a fusion polypeptide is provided that comprises a Light Chain, a Heavy Chain or both a Light and Heavy Chain. In another embodiment, the fusion polypeptide contains a heterologous immunoglobulin constant region. In another embodiment, the fusion polypeptide contains a VH and a VL Domain of an antibody produced from a publicly-deposited hybridoma. For purposes of this invention, an antibody fusion protein contains polypeptide domains that enable the protein to immunospecifically bind both CD16 and a Disease Antigen, and which contains another amino acid sequence to which it is not attached in the native molecule, for example, a heterologous sequence or a homologous sequence from another region (e.g., a deimmunized albumin-binding domain, a Protein A recognition sequence, a peptide tag, etc.). 
     The present invention particularly encompasses such binding molecules (e.g., antibodies, diabodies, trivalent binding molecules, etc.) conjugated to a diagnostic or therapeutic moiety. For diagnostic purposes, the binding molecules of the invention may be coupled to a detectable substance. Such binding molecules are useful for monitoring and/or prognosing the development or progression of a disease as part of a clinical testing procedure, such as determining the efficacy of a particular therapy. Examples of detectable substances include various enzymes (e.g., horseradish peroxidase, beta-galactosidase, etc.), prosthetic groups (e.g., avidin/biotin), fluorescent materials (e.g., umbelliferone, fluorescein, or phycoerythrin), luminescent materials (e.g., luminol), bioluminescent materials (e.g., luciferase or aequorin), radioactive materials (e.g., carbon-14, manganese-54, strontium-85 or zinc-65), positron emitting metals, and nonradioactive paramagnetic metal ions. The detectable substance may be coupled or conjugated either directly to the binding molecule or indirectly, through an intermediate (e.g., a linker) using techniques known in the art. 
     For therapeutic purposes, the binding molecules of the invention may be conjugated to a therapeutic moiety such as a cytotoxin, (e.g., a cytostatic or cytocidal agent), a therapeutic agent or a radioactive metal ion, e.g., alpha-emitters. A cytotoxin or cytotoxic agent includes any agent that is detrimental to cells such as, for example,  Pseudomonas  exotoxin, Diptheria toxin, a botulinum toxin A through F, ricin abrin, saporin, and cytotoxic fragments of such agents. A therapeutic agent includes any agent having a therapeutic effect to prophylactically or therapeutically treat a disorder. Such therapeutic agents may be chemical therapeutic agents, protein or polypeptide therapeutic agents, and include therapeutic agents that possess a desired biological activity and/or modify a given biological response. Examples of therapeutic agents include alkylating agents, angiogenesis inhibitors, anti-mitotic agents, hormone therapy agents, and antibodies useful for the treatment of cell proliferative disorders. The therapeutic moiety may be coupled or conjugated either directly to the binding molecule or indirectly, through an intermediate (e.g., a linker) using techniques known in the art. 
     IX. Uses of the Binding Molecules of the Present Invention 
     As discussed above, molecules capable of binding CD16 and a Disease Antigen are capable of mediating the redirected cell killing of a target cell (i.e., a cancer cell, or a pathogen-infected cell) that expresses such Disease Antigen on its cell surface. Such molecules may be used for therapeutic purposes, for example in subjects with cancer or an infection. Thus, binding molecules of the present invention have the ability to treat any disease or condition associated with or characterized by the expression of a Disease Antigen, particularly a Cancer Antigen or a Pathogen-Associated Antigen, on the surface of such target cell. Thus, without limitation, the binding molecules of the present invention may be employed in the treatment of cancer, particularly a cancer characterized by the expression of a Cancer Antigen. The binding molecules of the present invention may be employed in the treatment of infection, particularly an infection characterized by the expression of a Pathogen-Associated Antigen. 
     In particular, the present invention encompasses such methods wherein the molecule capable of binding CD16 comprises an Epitope-Binding Domain of an antibody that is capable of binding CD16 and also comprises an Epitope-Binding Domain capable of binding a Disease Antigen (in particular a Cancer Antigen or a Pathogen-Associated Antigen) on the surface of a target cell so as to mediate the redirected killing of the target cell (for example, by mediating redirected cell killing (e.g., redirected T-cell or redirected NK-cell cytotoxicity)). 
     In a specific embodiment, the molecule capable of binding CD16 and the Disease Antigen is a bispecific antibody, or the binding portions thereof, (including an scFv), a BiTe, a TandAb, and a CAR. 
     In a specific embodiment, the molecule capable of binding CD16 and the Disease Antigen is a bispecific diabody. 
     In a specific embodiment, the molecule capable of binding CD16 and the Disease Antigen is a trivalent binding molecule. 
     As used herein, the terms: “providing a therapy” and “treating” refer to any administration of a composition that is associated with any indicia of beneficial or desired result, including, without limitation, any clinical result such as decreasing symptoms resulting from the disease, attenuating a symptom of infection (e.g., viral load, fever, pain, sepsis, etc.) a shrinking of the size of a tumor (in the cancer context, for example, a tumor of breast, gastric or prostate cancer), a retardation of cancer cell growth, a delaying of the onset, development or progression of metastasis, a decreasing of a symptom resulting from the disease, an increasing of the quality of life of the recipient subject, a decreasing of the dose of other medications being provided to treat a subject&#39;s disease, an enhancing of the effect of another medication such as via targeting and/or internalization, a delaying of the progression of the disease, and/or a prolonging of the survival of recipient subject. 
     Subjects for treatment include animals, most preferably mammalian species such as non-primate (e.g., bovine, equine, feline, canine, rodent, etc.) or a primate (e.g., monkey such as, a cynomolgus monkey, human, etc.). In a preferred embodiment, the subject is a human. 
     Exemplary disorders that may be treated by various embodiments of the present invention include, but are not limited to, proliferative disorders, cell proliferative disorders, and cancer (especially a cancer expressing a Cancer Antigen bound by a molecule capable of mediating redirected cell killing), pathogen-associated diseases (especially a chronic viral infection associated with expression of a Pathogen-Associated Antigen bound by a molecule capable of mediating redirected cell killing). In various embodiments, the invention encompasses methods and compositions for treatment, prevention or management of a disease or disorder in a subject, comprising administering to the subject a therapeutically effective amount the binding molecules of the present invention. Such molecules are particularly useful for the prevention, inhibition, reduction of growth, or regression of primary tumors, and metastasis of tumors, and for reducing pathogen load, or eliminating pathogen-infected cells. Although not intending to be bound by a particular mechanism of action, such molecules may mediate effector function against target cells, promote the activation of the immune system against target cells, cross-link cell-surface antigens and/or receptors on target cells and enhance apoptosis or negative growth regulatory signaling, or a combination thereof, resulting in clearance and/or reduction in the number of target cells. 
     The cancers that may be treated by molecules of the present invention, and by the methods of the present invention, include, but are not limited to: an adrenal gland cancer, including but not limited to, a pheochromocytom or an adrenocortical carcinoma; an AIDS-associated cancer; an alveolar soft part sarcoma; an astrocytic tumor; a basal cancer; a bladder cancer, including but not limited to, a transitional cell carcinoma, a squamous cell cancer, an adenocarcinoma, or a carcinosarcoma; a bone and connective tissue sarcoma, such as but not limited to, a bone sarcoma, osteosarcoma, chondrosarcoma, Ewing&#39;s sarcoma, malignant giant cell tumor, fibrosarcoma of bone, chordoma, periosteal sarcoma, soft-tissue sarcomas, angiosarcoma (hemangiosarcoma), fibrosarcoma, Kaposi&#39;s sarcoma, leiomyosarcoma, liposarcoma, lymphangiosarcoma, neurilemmoma, rhabdomyosarcoma, or a synovial sarcoma; a brain cancer, including, but not limited to, a glioma, astrocytoma, brain stem glioma, ependymoma, oligodendroglioma, nonglial tumor, acoustic neurinoma, craniopharyngioma, medulloblastoma, meningioma, pineocytoma, pineoblastoma, or a primary brain lymphoma; a brain and spinal cord cancer; a breast cancer, including, but not limited to, an adenocarcinoma, lobular (small cell) carcinoma, intraductal carcinoma, medullary breast cancer, mucinous breast cancer, tubular breast cancer, papillary breast cancer, Paget&#39;s disease, or an inflammatory breast cancer; a carotid body tumor; a cervical cancer, including but not limited to, a squamous cell carcinoma, or a adenocarcinoma; a cholangiocarcinoma, including but not limited to, a papillary, nodular, or diffuse cholangiocarcinoma; a chondrosarcoma; a chordoma; a chromophobe renal cell carcinoma; a clear cell carcinoma; a colon cancer; a colorectal cancer; a cutaneous benign fibrous histiocytoma; a desmoplastic small round cell tumor; an ependymoma; an eye cancer, including, but not limited to, an ocular melanoma such as iris melanoma, choroidal melanoma, and cilliary body melanoma, and retinoblastoma; an esophageal cancer, including but not limited to, a squamous cancer, adenocarcinoma, adenoid cyctic carcinoma, mucoepidermoid carcinoma, adenosquamous carcinoma, sarcoma, melanoma, plasmacytoma, verrucous carcinoma, and an oat cell (small cell) carcinoma; a Ewing&#39;s tumor; an extraskeletal myxoid chondrosarcoma; a fibrogenesis imperfecta ossium; a fibrous dysplasia of the bone; a gallbladder or bile duct cancer, including but not limited to, an adenocarcinoma; a gastric cancer; a gestational trophoblastic disease; a germ cell tumor; a head and neck cancer; a hepatocellular carcinoma; Heavy Chain disease; an islet cell tumor; a Kaposi&#39;s sarcoma; a leukemia, including, but not limited to, an acute leukemia; acute lymphocytic leukemia; an acute myelocytic leukemia, such as, but not limited to, a myeloblastic, promyelocytic, myelomonocytic, monocytic, or erythroleukemia leukemia or a myelodysplastic syndrome; a chronic leukemia, such as but not limited to, a chronic myelocytic (granulocytic) leukemia, a chronic lymphocytic leukemia, a hairy cell leukemia; a lipoma/benign lipomatous tumor; a liposarcoma/malignant lipomatous tumor; a liver cancer, including but not limited to, a hepatocellular carcinoma, or a hepatoblastoma; a lymphoma, such as but not limited to, Hodgkin&#39;s disease; non-Hodgkin&#39;s disease; a lung cancer, including but not limited to, a non-small cell lung cancer, squamous cell carcinoma (epidermoid carcinoma), adenocarcinoma, large-cell carcinoma or a small-cell lung cancer; a medulloblastoma; a melanoma; a meningioma; a benign monoclonal gammopathy; a monoclonal gammopathy of undetermined significance; a multiple endocrine neoplasia; a multiple myeloma, such as but not limited to, a smoldering multiple myeloma, nonsecretory myeloma, osteosclerotic myeloma, plasma cell leukemia, solitary plasmacytoma and extramedullary plasmacytoma; a myelodysplastic syndrome; a neuroblastoma; a neuroendocrine tumor; an oral cancer, including but not limited to, a squamous cell carcinoma; an ovarian cancer; including, but not limited to, an ovarian epithelial carcinoma, borderline tumor, germ cell tumor, and stromal tumor; a pancreatic cancer, including but not limited to, an insulinoma, gastrinoma, glucagonoma, vipoma, somatostatin-secreting tumor, or a carcinoid or islet cell tumor; a parathyroid tumor; a pediatric cancer; a penal cancer; a peripheral nerve sheath tumor; a phaeochromocytoma; a pharynx cancer, including but not limited to, a squamous cell cancer, or a verrucous cancer; a pituitary cancer, including but not limited to, Cushing&#39;s disease, a prolactin-secreting tumor, acromegaly, or a diabetes insipius tumor; a prostate cancer, including but not limited to, an adenocarcinoma, leiomyosarcoma, or rhabdomyosarcoma; polycythemia vera; a posterious uveal melanoma; a rare hematologic disorder; a renal cancer, including but not limited to, an adenocarcinoma, hypernephroma, fibrosarcoma, a renal metastatic cancer, or a transitional cell cancer (renal pelvis and/or uterer); a rhabdoid tumor; a rhabdomysarcoma; a salivary gland cancer, including but not limited to, an adenocarcinoma, mucoepidermoid carcinoma, or an adenoidcystic carcinoma; a sarcoma; a skin cancer, including but not limited to, a basal cell carcinoma, a squamous cell carcinoma and melanoma, a superficial spreading melanoma, a nodular melanoma, a lentigo malignant melanoma, or an acral lentiginous melanoma; a soft-tissue sarcoma; a squamous cell cancer; a stomach cancer, including but not limited to, an adenocarcinoma, a fungating (polypoid), ulcerating, superficial spreading, diffusely spreading, or malignant lymphoma, liposarcoma, fibrosarcoma, and carcinosarcoma; a synovial sarcoma; a testicular cancer, including but not limited to, a germinal tumor, seminoma, anaplastic, classic (typical), spermatocytic, nonseminoma, embryonal carcinoma, teratoma carcinoma, or a choriocarcinoma (yolk-sac tumor); a thymic carcinoma; a thymoma; a thyroid cancer, such as but not limited to, papillary or follicular thyroid cancer, metastatic thyroid cancer, medullary thyroid cancer or anaplastic thyroid cancer; a uterine cancer, including but not limited to, an endometrial carcinoma or a uterine sarcoma; a vaginal cancer, including but not limited to, a squamous cell carcinoma, adenocarcinoma, or melanoma; a vulvar cancer, including but not limited to, a squamous cell carcinoma, melanoma, adenocarcinoma, basal cell carcinoma, sarcoma, or Paget&#39;s disease; a Waldenström&#39;s macroglobulinemia, or Wilms&#39; tumor. In addition, cancers include myxosarcoma, osteogenic sarcoma, endotheliosarcoma, lymphangio-endotheliosarcoma, mesothelioma, synovioma, hemangioblastoma, epithelial carcinoma, cystadenocarcinoma, bronchogenic carcinoma, sweat gland carcinoma, sebaceous gland carcinoma, papillary carcinoma and papillary adenocarcinomas (for a review of such disorders, see Fishman et al., 1985, Medicine, 2d Ed., J.B. Lippincott Co., Philadelphia and Murphy et al., 1997, Informed Decisions: The Complete Book of Cancer Diagnosis, Treatment, and Recovery, Viking Penguin, Penguin Books U.S.A., Inc.). 
     In particular, the binding molecules of the present invention may be used in the treatment of adrenal cancer, bladder cancer, breast cancer, colorectal cancer, gastric cancer, glioblastoma, kidney cancer, non-small-cell lung cancer, acute lymphocytic leukemia, acute myeloid leukemia, chronic lymphocytic leukemia, chronic myeloid leukemia, hairy cell leukemia, Burkett&#39;s lymphoma, diffuse large B cell lymphoma, follicular lymphoma, mantle cell lymphoma, marginal zone lymphoma, non-Hodgkin&#39;s lymphoma, small lymphocytic lymphoma, multiple myeloma, melanoma, ovarian cancer, pancreatic cancer, prostate cancer, skin cancer, renal cell carcinoma, testicular cancer, and uterine cancer. 
     Pathogen-associated diseases that may be treated by the CD16 Binding Molecules of the present invention include chronic viral, bacterial, fungal and parasitic infections. Chronic infections that may be treated by the CD16 Binding Molecules of the present invention include Epstein Barr virus, Hepatitis A Virus (HAV); Hepatitis B Virus (HBV); Hepatitis C Virus (HCV); herpes viruses (e.g. HSV-1, HSV-2, CMV), Human Immunodeficiency Virus (HIV), Vesicular Stomatitis Virus (VSV), Bacilli,  Citrobacter , Cholera, Diphtheria,  Enterobacter , Gonococci,  Helicobacter pylori, Klebsiella, Legionella , Meningococci, mycobacteria,  Pseudomonas , Pneumonococci,  rickettsia  bacteria,  Salmonella, Serratia , Staphylococci, Streptococci, Tetanus,  Aspergillus  ( fumigatus, niger , etc.),  Blastomyces dermatitidis, Candida  ( albicans, krusei, glabrata, tropicalis , etc.),  Cryptococcus neoformans , Genus Mucorales ( mucor, absidia, rhizopus ),  Sporothrix schenkii, Paracoccidioides brasiliensis, Coccidioides immitis, Histoplasma capsulatum , Leptospirosis,  Borrelia burgdorferi , helminth parasite (hookworm, tapeworms, flukes, flatworms (e.g. Schistosomia), Giardia Zambia,  trichinella, Dientamoeba Fragilis, Trypanosoma brucei, Trypanosoma cruzi , and  Leishmania donovani ). 
     X. Pharmaceutical Compositions 
     The present invention encompasses compositions comprising a molecule capable of binding CD16 and also capable of binding to a Disease Antigen. The compositions of the invention include bulk drug compositions useful in the manufacture of pharmaceutical compositions (e.g., impure or non-sterile compositions) and pharmaceutical compositions (i.e., compositions that are suitable for administration to a subject or patient) that can be used in the preparation of unit dosage forms. Such compositions comprise a prophylactically or therapeutically effective amount of a molecule capable of binding CD16 and also capable of binding to a Disease Antigen so as to be capable of mediating the redirected killing of a target cell (e.g., a cancer cell, a pathogen-infected cell, etc.), or a combination of such agents and a pharmaceutically acceptable carrier. Preferably, compositions of the invention comprise a prophylactically or therapeutically effective amount of the binding molecules of the present invention and a pharmaceutically acceptable carrier. In a preferred aspect, such compositions are substantially purified (i.e., substantially free from substances that limit its effect or produce undesired side effects). 
     Various formulations of such compositions may be used for administration. In addition to the pharmacologically active agent(s), the compositions of the present invention may contain suitable pharmaceutically acceptable carriers comprising excipients and auxiliaries that are well-known in the art and are relatively inert substances that facilitate administration of a pharmacologically effective substance or which facilitate processing of the active compounds into preparations that can be used pharmaceutically for delivery to the site of action. For example, an excipient can give form or consistency, or act as a diluent. Suitable excipients include but are not limited to stabilizing agents, wetting and emulsifying agents, salts for varying osmolarity, encapsulating agents, buffers, and skin penetration enhancers. 
     In a specific embodiment, the term “pharmaceutically acceptable” means approved by a regulatory agency of the Federal or a state government or listed in the U.S. Pharmacopeia or other generally recognized pharmacopeia for use in animals, and more particularly in humans. The term “carrier” refers to a diluent, adjuvant (e.g., Freund&#39;s adjuvant (complete and incomplete), excipient, or vehicle with which the therapeutic is administered. Generally, the ingredients of compositions of the invention are supplied either separately or mixed together in unit dosage form, for example, as a dry lyophilized powder or water free concentrate in a hermetically sealed container such as an ampoule or sachette indicating the quantity of active agent. Where the composition is to be administered by infusion, it can be dispensed with an infusion bottle containing sterile pharmaceutical grade water or saline. Where the composition is administered by injection, an ampoule of sterile water for injection or saline can be provided so that the ingredients may be mixed prior to administration. 
     The invention also provides a pharmaceutical pack or kit comprising one or more containers filled with a binding molecule of the present invention, alone or with such pharmaceutically acceptable carrier. Additionally, one or more other prophylactic or therapeutic agents useful for the treatment of a disease can also be included in the pharmaceutical pack or kit. The invention also provides a pharmaceutical pack or kit comprising one or more containers filled with one or more of the ingredients of the pharmaceutical compositions of the invention. Optionally associated with such container(s) can be a notice in the form prescribed by a governmental agency regulating the manufacture, use or sale of pharmaceuticals or biological products, which notice reflects approval by the agency of manufacture, use or sale for human administration. 
     The present invention provides kits that can be used in the above methods. A kit can comprise any of the binding molecules of the present invention. The kit can further comprise one or more other prophylactic and/or therapeutic agents useful for the treatment of cancer, in one or more containers. 
     XI. Methods of Administration 
     The compositions of the present invention may be provided for the treatment, prophylaxis, and amelioration of one or more symptoms associated with a disease, disorder or infection by administering to a subject an effective amount of a CD16 x DA pharmaceutical composition of the present invention. In a preferred aspect, such compositions are substantially purified (i.e., substantially free from substances that limit its effect or produce undesired side effects). In a specific embodiment, the subject is an animal, preferably a mammal such as non-primate (e.g., bovine, equine, feline, canine, rodent, etc.) or a primate (e.g., monkey such as, a cynomolgus monkey, human, etc.). In a preferred embodiment, the subject is a human. 
     Methods of administering a CD16 x DA Binding Molecule or pharmaceutical composition of the invention include, but are not limited to, parenteral administration (e.g., intradermal, intramuscular, intraperitoneal, intravenous and subcutaneous), epidural, and mucosal (e.g., intranasal and oral routes). In a specific embodiment, the binding molecules of the present invention are administered intramuscularly, intravenously, or subcutaneously. The compositions may be administered by any convenient route, for example, by infusion or bolus injection, by absorption through epithelial or mucocutaneous linings (e.g., oral mucosa, rectal and intestinal mucosa, etc.) and may be administered together with other biologically active agents. Administration can be systemic or local. 
     The invention also provides that preparations of the binding molecules of the present invention are packaged in a hermetically sealed container such as an ampoule or sachette indicating the quantity of the molecule. In one embodiment, such molecules are supplied as a dry sterilized lyophilized powder or water free concentrate in a hermetically sealed container and can be reconstituted, e.g., with water or saline to the appropriate concentration for administration to a subject. Preferably, the binding molecules of the present invention are supplied as a dry sterile lyophilized powder in a hermetically sealed container. 
     The lyophilized preparations of the binding molecules of the present invention should be stored at between 2° C. and 8° C. in their original container and the molecules should be administered within 12 hours, preferably within 6 hours, within 5 hours, within 3 hours, or within 1 hour after being reconstituted. In an alternative embodiment, such molecules are supplied in liquid form in a hermetically sealed container indicating the quantity and concentration of the molecule, fusion protein, or conjugated molecule. Preferably, such binding molecules, when provided in liquid form, are supplied in a hermetically sealed container. 
     The amount of such preparations of the invention that will be effective in the treatment, prevention or amelioration of one or more symptoms associated with a disorder can be determined by standard clinical techniques. The precise dose to be employed in the formulation will also depend on the route of administration, and the seriousness of the condition, and should be decided according to the judgment of the practitioner and each patient&#39;s circumstances. Effective doses may be extrapolated from dose-response curves derived from in vitro or animal model test systems. 
     As used herein, an “effective amount” of a pharmaceutical composition is an amount sufficient to effect beneficial or desired results including, without limitation, clinical results such as decreasing symptoms resulting from the disease, attenuating a symptom of infection (e.g., viral load, fever, pain, sepsis, etc.) or a symptom of cancer (e.g., the proliferation, of cancer cells, tumor presence, tumor metastases, etc.), thereby increasing the quality of life of those suffering from the disease, decreasing the dose of other medications required to treat the disease, enhancing the effect of another medication such as via targeting and/or internalization, delaying the progression of the disease, and/or prolonging survival of individuals. When applied to an individual active ingredient, administered alone, the term refers to that ingredient alone. When applied to a combination, the term refers to combined amounts of the active ingredients that result in the therapeutic effect, whether administered in combination, serially, or simultaneously. 
     An effective amount can be administered in one or more administrations. For purposes of this invention, an effective amount of drug, compound, or pharmaceutical composition is an amount sufficient: to kill and/or reduce the proliferation of cancer cells, and/or to eliminate, reduce and/or delay the development of metastasis from a primary site of cancer; or to reduce the proliferation of (or the effect of) an infectious pathogen and to reduce and/or delay the development of the pathogen-mediated disease, either directly or indirectly. In some embodiments, an effective amount of a drug, compound, or pharmaceutical composition may or may not be achieved in conjunction with another drug, compound, or pharmaceutical composition. Thus, an “effective amount” may be considered in the context of administering one or more chemotherapeutic agents, and a single agent may be considered to be given in an effective amount if, in conjunction with one or more other agents, a desirable result may be or is achieved. While individual needs vary, determination of optimal ranges of effective amounts of each component is within the skill of the art. 
     For the binding molecules encompassed by the invention, the dosage administered to a patient is preferably determined based upon the body weight (kg) of the recipient subject. For the binding molecules encompassed by the invention, the dosage administered to a patient is typically from about 0.01 μg/kg to about 30 mg/kg or more of the subject&#39;s body weight. 
     The dosage and frequency of administration of a binding molecule of the present invention may be reduced or altered by enhancing uptake and tissue penetration of the molecule by modifications such as, for example, lipidation. 
     The dosage of a binding molecule of the invention administered to a patient may be calculated for use as a single agent therapy. Alternatively, the molecule may be used in combination with other therapeutic compositions and the dosage administered to a patient are lower than when said molecules are used as a single agent therapy. 
     The pharmaceutical compositions of the invention may be administered locally to the area in need of treatment; this may be achieved by, for example, and not by way of limitation, local infusion, by injection, or by means of an implant, said implant being of a porous, non-porous, or gelatinous material, including membranes, such as SIALASTIC® membranes, or fibers. Preferably, when administering a molecule of the invention, care must be taken to use materials to which the molecule does not absorb. 
     The compositions of the invention can be delivered in a vesicle, in particular a liposome (See Langer (1990) “ New Methods Of Drug Delivery ,” Science 249:1527-1533); Treat et al., in L IPOSOMES IN THE  T HERAPY OF  I NFECTIOUS  D ISEASE AND  C ANCER , Lopez-Berestein and Fidler (eds.), Liss, New York, pp. 353-365 (1989); Lopez-Berestein, ibid., pp. 317-327). 
     Treatment of a subject with a therapeutically or prophylactically effective amount of a binding molecule of the present invention can include a single treatment or, preferably, can include a series of treatments. In a preferred example, a subject is treated with a pharmaceutical composition of the invention for between about 1 to 10 weeks, preferably between 2 to 8 weeks, more preferably between about 3 to 7 weeks, and even more preferably for about 4, 5, or 6 weeks. The pharmaceutical compositions of the invention can be administered once a day with such administration occurring once a week, twice a week, once every two weeks, once a month, once every six weeks, once every two months, twice a year or once per year, etc. Alternatively, the pharmaceutical compositions of the invention can be administered twice a day with such administration occurring once a week, twice a week, once every two weeks, once a month, once every six weeks, once every two months, twice a year or once per year, etc. Alternatively, the pharmaceutical compositions of the invention can be administered three times a day with such administration occurring once a week, twice a week, once every two weeks, once a month, once every six weeks, once every two months, twice a year or once per year, etc. It will also be appreciated that the effective dosage of the molecules used for treatment may increase or decrease over the course of a particular treatment. 
     EXAMPLES 
     Having now generally described the invention, the same will be more readily understood through reference to the following examples, which are provided by way of illustration and are not intended to be limiting of the present invention unless specified. 
     Example 1 
     Characterization of CD16 Binding Molecules 
     As discussed above, a series of DART® Diabody CD16 x DA Binding Molecules were generated incorporating different murine, humanized, or optimized anti-CD16 binding domains and having a Binding Domain that is immunospecific for either: (1) the HER2/neu tumor antigen (Binding Domain from Trastuzumab), (2) a Binding Domain that is immunospecific for an HIV antigen (Binding Domain from A32 or 7B2), (3) the CD19 B-cell antigen (Binding Domain from CD19 mAb 1), or (4) a Binding Domain that is immunospecific for an RSV antigen (Binding Domain from Palivizumab) (Table 14). 
     
       
         
           
               
               
               
               
             
               
                 TABLE 14 
               
               
                   
               
               
                 Designation 
                 Specificities 
                 CD16 VH/VL 
                 Target VH/VL 
               
               
                   
               
             
            
               
                 DART-A 
                 CD16 × HER2/neu 
                 CD16-M1 
                 Trastuzumab 
               
               
                 DART-B 
                 CD16 × HER2/neu 
                 CD16-M2 
                 Trastuzumab 
               
               
                 DART-C 
                 CD16 × HER2/neu 
                 hCD16-M1 
                 Trastuzumab 
               
               
                 DART-D 
                 CD16 × HER2/neu 
                 hCD16-M2 (VH1) 
                 Trastuzumab 
               
               
                 DART-E 
                 CD16 × HER2/neu 
                 hCD16-M2 (VH2) 
                 Trastuzumab 
               
               
                 DART-F 
                 CD16 × HIV 
                 hCD16-M1 
                 A32 
               
               
                 DART-G 
                 CD16 × HIV 
                 hCD16-M2 (VH1) 
                 A32 
               
               
                 DART-H 
                 CD16 × HIV 
                 hCD16-M1 
                 7B2 
               
               
                 DART-I 
                 CD16 × HER2/neu 
                 hCD16-M1A 
                 Trastuzumab 
               
               
                 DART-J 
                 CD16 × HER2/neu 
                 hCD16-M1B 
                 Trastuzumab 
               
               
                 DART-K 
                 CD16 × HER2/neu 
                 hCD16-M1AB 
                 Trastuzumab 
               
               
                 DART-L 
                 CD16 × CD19 
                 hCD16-M1 
                 CD19 mAb 1 
               
               
                 DART-M 
                 CD16 × CD19 
                 hCD16-M1B 
                 CD19 mAb 1 
               
               
                 DART-N 
                 CD16 × CD19 
                 hCD16-M1AB 
                 CD19 mAb 1 
               
               
                 DART-1 
                 CD16 × HER2/neu 
                 h3G8 
                 Trastuzumab 
               
               
                 DART-2 
                 CD16 × RSV 
                 h3G8 
                 Palivizumab  
               
               
                   
                   
                   
                 variant 
               
               
                 DART-3 
                 CD16 × RSV 
                 hCD16-M1 
                 vPalivizumab 
               
               
                 DART-4 
                 CD16 × HIV 
                 h3G8 
                 7B2 
               
               
                 DART-5 
                 CD16 × RSV 
                 hCD16-M1 
                 vPalivizumab 
               
               
                 DART-6 
                 CD16 × RSV 
                 hCD16-M1B 
                 vPalivizumab 
               
               
                 DART-7 
                 CD16 × RSV 
                 hCD16-M1AB 
                 vPalivizumab 
               
               
                 DART-X 
                 CD16 × HIV 
                 CD16-M1 
                 7B2 
               
               
                 DART-Y 
                 CD16 × HIV 
                 CD16-M2 
                 7B2 
               
               
                 DART-Z 
                 CD16 × HIV 
                 4-LSN1 
                 A32 
               
               
                 DART-0 
                 CD16 × HIV 
                 h3G8 
                 7B2 
               
               
                   
               
            
           
         
       
     
     Competition studies were performed with the two chain diabodies (DART-X, DART-Y and DART-0) comprising murine antibody CD16-M1, murine antibody CD16-M2, humanized antibody 3G8 and two widely-available commercial antibodies: LNK16 and DJ130c (Abcam, etc.) A ProteOn analysis was conducted to assess the binding of test articles to CD16 molecules that had been immobilized on a chip. The CD16 molecules were first incubated with 5 nM CD16-Fc (human IgG2) Fusion captured, followed by incubation with 10 nM of a first test article (test article 1), followed by an incubation with 10 nM of a second test article (test article 2). Binding was detected using anti-human Fc F(ab′)2 fragments. 
     This study revealed that the CD16-M1, CD16-M2, LNK16 and 3G8 binding domains each bound different epitopes of CD16. CD16-M2 was found to compete with antibody DJ130c for binding to CD16; DJ130c has been reported to bind CD16 at a site that is non-overlapping with CD16&#39;s Fc binding site (Tamm, A. et al. (1996) “ The Binding Epitopes Of Human CD 16 ( Fc Gamma RIII )  Monoclonal Antibodies. Implications For Ligand Binding ,” J. Immunol. 157(4):1576-1581; Tamm, A. et al. (1996) “The  Igg Binding Site Of Human FcgammaRIIIB Receptor Involves CC′ And FG Loops Of The Membrane - Proximal Domain ,” J. Biol. Chem. 271(7):3659-3666). 
     The ability of CD16-M1, DJ130c and LNK16 to bind CD16A in presence of IgG was evaluated. It was found that IgG presence inhibited binding by NK16, but that IgG presence did not inhibit binding between CD16 and CD16-M1 was not inhibited by the presence of IgG, whereas L. The 3G8 epitope has been reported to overlap with CD16&#39;s Fc binding site (Tamm, A. et al. (1996) J. Biol. Chem. 271(7):3659-3666). 
     Increasing concentrations of human IgG (hIgG) descreased the binding of antibody LNK16 to CD16, thus indicating that the epitope recognized by LNK16 overlaps with CD16&#39;s Fc binding site; in contrast, binding by the CD16 Binding domains of DJ130c or CD16-M1 was substantially unaffected, thus indicating that the CD16 epitopes recognized by these molecules did not overlap with CD16&#39;s Fc binding site ( FIG. 8 ). 
     As discussed above, CD16A possesses two major allotypes, reflecting an 158F vs. 158V polymorphism. As shown in Table 15, CD16 Binding Domains of CD16-M1 and CD16-M2 were found to bind to both CD16 allotypes with similar high affinity (approximately 3-25-fold better than h3G8, which has a 10-fold lower affinity to the CD16 158F allotype, relative to the CD16 158V allotype. The binding domain of h3G8 was found to exhibit a fast off-rate. The analysis was conducted using a BIACORE® format in which the diabody molecules were captured on a goat (Fab′)2 anti-human Fc surface, and the human CD16A V/F molecules (labeled with Avitag) were passed over the captured diabodies (normalized; 1:1 binding fit). 
     
       
         
           
               
               
               
               
               
             
               
                   
                 TABLE 15 
               
               
                   
                   
               
               
                   
                 Molecule 
                 ka 
                 kd 
                 K D  (nM) 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Human CD16A 158F 
               
            
           
           
               
               
               
               
               
            
               
                   
                 DART-1 
                 1.7 × 10 6   
                 1.1 × 10 −1   
                 65.9 
               
               
                   
                 DART-C 
                 1.6 × 10 6   
                 3.0 × 10 −4   
                 0.2 
               
               
                   
                 DART-D 
                 4.7 × 10 5   
                 1.2 × 10 −3   
                 2.5 
               
               
                   
                 DART-F 
                 1.5 × 10 6   
                 3.4 × 10 −4   
                 0.2 
               
               
                   
                 DART-G 
                 4.7 × 10 5   
                 1.2 × 10 −3   
                 2.6 
               
            
           
           
               
            
               
                 Human CD16A 158V 
               
            
           
           
               
               
               
               
               
            
               
                   
                 DART-1 
                 1.3 × 10 6   
                 9.0 × 10 −3   
                 6.9 
               
               
                   
                 DART-C 
                 1.4 × 10 6   
                 3.9 × 10 −4   
                 0.3 
               
               
                   
                 DART-D 
                 5.2 × 10 5   
                 1.2 × 10 −3   
                 2.3 
               
               
                   
                 DART-F 
                 1.4 × 10 6   
                 3.7 × 10 −4   
                 0.3 
               
               
                   
                 DART-G 
                 5.4 × 10 5   
                 1.2 × 10 −3   
                 2.2 
               
               
                   
                   
               
            
           
         
       
     
     A ProteOn analysis was conducted to assess the binding of the CD16 Binding Domains of the constructed diabodies to CD16-His tagged molecules (R&amp;D Systems) that had been immobilized on a chip. The CD16 molecules were immobilized via an anti-PentaHis antibody, followed by incubation incubation with the diabodies. The results of the CD16 binding studies are summarized in Table 16 (the CD16 Binding Domain of DART-Z derives from scFv 4-LS21, which is specific for CD16A). 
     
       
         
           
               
               
               
               
               
             
               
                   
                 TABLE 16 
               
               
                   
                   
               
             
            
               
                   
                 Molecule 
                 ka 
                 kd 
                 K D  (nM) 
               
               
                   
                   
               
            
           
           
               
            
               
                 Human CD16A V158F 
               
            
           
           
               
               
               
               
               
            
               
                   
                 DART-A 
                 4.2 × 10 6   
                 3.3 × 10 −4   
                 0.1 
               
               
                   
                 DART-B 
                 2.9 × 10 5   
                 4.8 × 10 −4   
                 1.7 
               
               
                   
                 DART-1 
                 5.3 × 10 5   
                 3.4 × 10 −2   
                 63.1 
               
               
                   
                 DART-C 
                 2.8 × 10 6   
                 2.6 × 10 −4   
                 0.1 
               
               
                   
                 DART-D 
                 4.3 × 10 5   
                 1.0 × 10 −3   
                 2.4 
               
               
                   
                 DART-E 
                 2.3 × 10 5   
                 1.6 × 10 −3   
                 7.2 
               
               
                   
                 DART-F 
                 4.2 × 10 6   
                 2.4 × 10 −4   
                 0.1 
               
               
                   
                 DART-G 
                 4.7 × 10 5   
                 8.9 × 10 −4   
                 1.9 
               
               
                   
                 DART-H 
                 4.5 × 10 6   
                 1.9 × 10 −4   
                 0.04 
               
               
                   
                 DART-4 
                 6.1 × 10 5   
                 4.7× 10 −2   
                 76.8 
               
               
                   
                 DART-Z 
                 2.9 × 10 5   
                 4.3 × 10 −3   
                 15.2 
               
            
           
           
               
            
               
                 Human CD16A 158V 
               
            
           
           
               
               
               
               
               
            
               
                   
                 DART-A 
                 3.9 × 10 6   
                 4.5 × 10 −4   
                 0.1 
               
               
                   
                 DART-B 
                 3.2 × 10 5   
                 4.1 × 10 −4   
                 1.3 
               
               
                   
                 DART-1 
                 2.4 × 10 5   
                 6.5 × 10 −3   
                 26.7 
               
               
                   
                 DART-C 
                 2.4 × 10 6   
                 3.2 × 10 −4   
                 0.1 
               
               
                   
                 DART-D 
                 5.6 × 10 5   
                 7.1 × 10 −4   
                 1.3 
               
               
                   
                 DART-E 
                 3.3 × 10 5   
                 1.1 × 10 −3   
                 3.3 
               
               
                   
                 DART-F 
                 4.0 × 10 6   
                 4.0 × 10 −4   
                 0.1 
               
               
                   
                 DART-G 
                 6.6 × 10 5   
                 5.5 × 10 −4   
                 0.8 
               
               
                   
                 DART-H 
                 3.9 × 10 6   
                 3.7 × 10 −4   
                 0.1 
               
               
                   
                 DART-4 
                 2.9 × 10 6   
                 6.9 × 10 −3   
                 23.3 
               
               
                   
                 DART-Z 
                 2.6 × 10 5   
                 4.2 × 10 −3   
                 15.7 
               
            
           
           
               
            
               
                 Human CD16B 
               
            
           
           
               
               
               
               
               
            
               
                   
                 DART-A 
                 2.6 × 10 6   
                 1.3 × 10 −3   
                 0.5 
               
               
                   
                 DART-B 
                 2.2 × 10 5   
                 7.4 × 10 −4   
                 3.3 
               
               
                   
                 DART-1 
                 8.9 × 10 5   
                 5.5 × 10 −3   
                 6.1 
               
               
                   
                 DART-C 
                 1.5 × 10 6   
                 6.9 × 10 −4   
                 0.5 
               
               
                   
                 DART-D 
                 3.5 × 10 5   
                 1.3 × 10 −3   
                 3.6 
               
               
                   
                 DART-E 
                 1.5 × 10 5   
                 1.9 × 10 −3   
                 12.5 
               
               
                   
                 DART-F 
                 2.8 × 10 6   
                 1.1 × 10 −3   
                 0.4 
               
               
                   
                 DART-G 
                 4.6 × 10 5   
                 1.2 × 10 −3   
                 2.6 
               
               
                   
                 DART-H 
                 2.5 × 10 6   
                 8.9 × 10 −4   
                 0.4 
               
               
                   
                 DART-4 
                 7.8 × 10 5   
                 6.1 × 10 −3   
                 7.8 
               
               
                   
                 DART-Z 
                 N/A* 
                 N/A* 
                 N/A* 
               
               
                   
                   
               
               
                   
                 * The CD16 Binding Domain of DART-Z derives from scFv 4-LS21, which is specific for CD16A 
               
            
           
         
       
     
     The results indicate that diabodies comprising CD16-M1 or CD16-M2 CD16 Binding Domains exhibited higher affinity to both alleles of CD16A and higher affinity than comparator molecules. Diabodies comprising hCD16-M2 (VH1) CD16 Binding Domains exhibited slightly better binding than diabodies comprising the hCD16-M2 (VH2) CD16 Binding Domains (compare DART-D vs. DART-E). 
     Example 2 
     Binding of DART-F and DART-G CD16 Binding Molecules to NK Cells, Neutrophils and T Cells 
     In order to demonstrate the ability of the CD16 x DA Binding Molecules of the invention to bind CD16, whole blood of donor subjects was incubated in the presence of CD16-binding diabodies: DART-F and DART-G, an HIV x CD3 diabody (as a positive control for T-cell binding via its CD3 Binding Domain), an HIV x RSV diabody (as a negative control for all binding), and an h3G8 x RSV diabody comparator molecule. Following incubation, the cells were labled with: anti-CD56-allophycocyanin (CD56 APC, NK cell marker), anti-CD66b-fluorescein isothiocyanate (CD66b FITC, neutrophil cell marker) and anti-CD3-peridinin chlorophyll protein-Cy5.5 (CD3 PerCP Cy5.5, T-cell marker), and anti-humanFc-Phycoerythrin (αhFc PE) to detect diabody binding and the cell surface. The labeled cells were analyzed by flow cytometry gated on NK cells. The co-staining results of the investigation for a first subject are shown in  FIGS. 9A-9C , and indicate that DART-F (hCD16-M1) had a higher affinity for CD16A on the surface of NK cells relative to DART-G (hCD16-M2), both of which had a higher affinity than the h3G8 x RSV diabody comparator molecule ( FIG. 9A ). In contrast, whereas the h3G8 x RSV diabody comparator molecule was able to bind to CD16B of neutrophils, DART-G had lower binding ability, and DART-F had essentially no binding ability ( FIG. 9B ). As shown in  FIG. 9C , the CD16 Binding Molecules were unable to bind to T cells. 
     Similar binding was observed using whole blood of a second donor subject. Lymphocyte binding (CD16A F/V) and Neutrophil binding (CD16B, allotype undetermined) was assessed essentially as described using DART-F (hCD16-M1), DART-G (hCD16-M1), and the h3G8 x RSV diabody comparator. The results of the investigation are shown in  FIGS. 10A-10B . As shown in  FIG. 10A , DART-F (hCD16-M1) and DART-G (hCD16-M1) both exhibited binding to CD16A-expressing cells of the gated lymphocyte population, with DART-F exhibiting greater binding; the h3G8 x RSV diabody comparator molecule exhibited much weaker binding. As shown in  FIG. 10B , DART-F failed to bind CD16B-expressing cells of the gated granulocyte population, in contrast to DART-G and the h3G8 x RSV diabody comparator molecule, which both exhibited binding. 
     Example 3 
     CD16B Allotype Specificity of Anti-CD16 Binding Molecules CD16-M1 and CD16-M2 
     In order to demonstrate the ability of the CD16 x DA Binding Molecules of the invention to distinguish glycosylation allotypes NA1 and NA2 of CD16B, DART-F (hCD16-M1) and the h3G8 x RSV diabody comparator were incubated in the presence of whole blood and analyzed essentially as described above. The results show diabody staining of whole blood that had been gated on CD66b+ cells (i.e., neutrophils and eosinophils).  FIGS. 11A-11B  show the results of such an analysis, and indicate that the h3G8 x RSV diabody comparator exhibited strong binding to NA1 and medium binding to NA2. In contrast, DART-F exhibited strong binding to NA2 and weak binding to NA1. 
     Example 4 
     CD16B Allotype Specificity of CD16 Binding Molecules CD16-M1 and CD16-M2 
     In order to further evaluate the ability of the CD16 x DA Binding Molecules of the invention to bind leukocytes, the ability of CD16 Binding Domains of CD16-M1 to bind NK cells and granulocytes from whole blood was assessed. DART-C was incubated in the presence of whole blood leukocyte cells and analyzed essentially as described above. The results show diabody staining of whole blood that had been gated on NK cells (0.6% of leukocytres) or on granulocytes (49% of leukocytes).  FIG. 12  shows the results of such an analysis, and indicates that CD16-M1 preferentially binds to NK cells. 
     Example 5 
     Binding of CD16 Binding Molecules to Human CD16, Cynomolgus Monkey CD16 and Murine CD16 
     Since the amino acid sequences of human CD16, cynomolgus monkey CD16 and murine CD16 share varying degrees of homology ( FIG. 13 ; SEQ ID NOs:183, 184, and 185, respectively), the ability of CD16 x DA Binding Molecules possessing CD16 Binding Domains of antibodies CD16-M1, CD16-M2 and 3G8 to bind human CD16, cynomolgus monkey CD16 and murine CD16 was investigated. The analysis was conducted using a BIACORE® format in which the diabodies molecules were captured on a Her2-His-tag surface, and the CD16 molecules (IgG2-Fc fusion proteins, 25 and 100 nM) were passed over the captured diabodies (normalized; bivalent binding fit estimate). The results of the investigation are summarized in Table 17. 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 17 
               
               
                   
               
               
                   
                   
                   
                 Cynomolgus 
                   
               
               
                 CD16 
                 CD16 
                 Human 
                 Monkey 
                 Murine 
               
               
                 Binding 
                 Binding 
                 CD16 158F 
                 CD16 
                 CD16 
               
               
                 Molecule 
                 Domain 
                 K D  (nM) 
                 K D  (nM) 
                 K D  (nM) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 DART-1 
                 h3G8 
                 13 
                 29 
                 No Binding 
               
               
                 DART-C 
                 hCD16-M1 
                 12 
                 571 
                 No Binding 
               
               
                 DART-D 
                 hCD16-M2 
                 4 
                 597 
                 No Binding 
               
               
                   
               
            
           
         
       
     
     The CD16 binding domain of h3G8 was found to be able to bind CD16a of cynomolgus monkey with somewhat reduced affinity relative to its binding to human CD16a. The CD16 binding domains of DART-C (hCD16-M1) and DART-D (hCD16-M2) were found to have low affinity to cynomolgus monkey CD16. None of the tested CD16 binding molecules were found to be capable of binding to murine CD16, commensurate with its lower homology to human CD16. 
     Example 6 
     Evaluation of CD16 Binding Molecule-Mediated Cytotoxicity of HER2/Neu-Positive Cells 
     In order to demonstrate the ability of the CD16 x DA Binding Molecules of the invention to mediate cell killing, the CD16 x HER2/neu Binding Molecules:
         DART-C (having an hCD16-M1 CD16 Binding Domain);   DART-D (having an hCD16-M2 CD16 Binding Domain);   DART-1 (having an h3G8 CD16 Binding Domain);
 
or a HER2/Neux RSV diabody (negative control) were separately incubated with target cancer cells expressing different levels of HER2/neu in the presence of Effector cells (either human PBMC (E:T=30:1) or purified human NK cells (E:T=2:1)) for 24 hours. The percentage cytotoxicity (i.e., redirected cell killing) was determined by measuring the release of lactate dehydrogenase (LDH) into the media by damaged cells essentially as described below. The results of this investigation are shown in  FIGS. 14A-14E .  FIG. 14A  shows the cytotoxicity exhibited against N87 HER2/neu target cells ((HER2/neu expression: +++) by purified NK cells; the CD16A allotype of such NK cells was 158F/158F.  FIG. 14B  shows the cytotoxicity exhibited against MCF7 HER2/neu target cells (HER2/neu expression: +/−) by purified NK cells; the CD16A allotype of such NK cells was 158F/158F.  FIG. 14C  shows the cytotoxicity exhibited against MDA-MB-231 HER2/neu target cells (HER2/neu expression: +/−) by PBMCs; the CD16A allotype of the NK cells of such PBMC preparation was not assessed.  FIG. 14D  shows the cytotoxicity exhibited against N87 HER2/neu target cells (HER2/neu expression: +++) by PBMCs; the CD16A allotype of the NK cells of such PBMC preparation was 158F/158V.  FIG. 14E  shows the cytotoxicity exhibited against Hs700T HER2/neu target cells (HER2/neu expression: +/−) by PBMCs; the CD16A allotype of the NK cells of such PBMC preparation was 158F/158V. The results show that CD16 x DA Binding Molecules comprising the hCD16-M1 CD16 Binding Domain exhibited greater cytotoxicity against the HER2/neu-expressing cancer cells than CD16 x DA Binding Molecules comprising the hCD16-M2 CD16 Binding Domain, and that molecules comprising such CD16 Binding Domains exhibited greater cytotoxicity than CD16 x DA Binding Molecules comprising the h3G8 CD16 Binding Domain.
       

     Example 7 
     Evaluation of CD16 Binding Molecule-Mediated Cytotoxicity of HIV-Infected Cells 
     In order to further demonstrate the ability of the CD16 x DA Binding Molecules of the invention to mediate cell killing, the CD16 x HIV env Binding Molecules:
         DART-F (having an hCD16-M1 CD16 Binding Domain);   DART-G (having an hCD16-M2 CD16 Binding Domain);
 
or DART-2 (a h3G8 x RSV diabody used as a negative control here)), were separately incubated with target 293HEK D371, which express HIV Env, in the presence of Effector cells (either human PBMC (E:T=30:1) or purified human NK cells (E:T=3:1) for 24 hours. The percentage cytotoxicity (i.e. redirected cell killing) was determined by measuring the release of lactate dehydrogenase (LDH) into the media by damaged cells using an LDH redirected cell killing assay essentially as described below. The results of this investigation are shown in  FIGS. 15A-15C .  FIG. 15A  shows the cytotoxicity exhibited against the 293HEK D371 target cells by PBMCs of a first donor; the CD16A allotype the NK cells of such PBMC preparation was 158F/158V.  FIG. 15B  shows the cytotoxicity exhibited against the 293HEK D371 target cells by PBMCs of a second donor; the CD16A allotype of the NK cells of such PBMC preparation was 158F/158F.  FIG. 15C  shows the cytotoxicity exhibited against the 293HEK D371 target cells MC by purified NK cells; the CD16A allotype of the NK cells was 158F/158V. The results again showed that CD16 x DA Binding Molecules comprising the hCD16-M1 CD16 Binding Domain exhibited greater cytotoxicity against the HIV env-espressing cells than CD16 x DA Binding Molecules comprising the hCD16-M2 CD16 Binding Domain.
       

     As indicated above, DART-F and DART-G are both Fc Domain-containing diabodies composed of three polypeptide chains. In order to demonstrate the ability of CD16 x DA Binding Molecules of the invention that lack Fc Domains to mediate cell killing, the CD16 x HIV env Binding Molecules:
         DART-X (having an hCD16-M1 CD16 Binding Domain);   DART-Y (having an hCD16-M2 CD16 Binding Domain);   DART-0 (having an h3G8 CD16 Binding Domain);
 
or DART-3 (a CD16 x RSV diabody having an hCD16-M1 CD16 Binding Domain used as a negative control here), were separately incubated with target HEK/D371 cells, which express the HIV env protein, in the presence of Effector cells (either Jurkat/CD16A 158F ( FIG. 16A ) or 158V/NFAT-Luc cells ( FIG. 16B )). The percentage cytotoxicity (i.e. cell killing) was determined by measuring the release of lactate dehydrogenase (LDH) into the media by damaged cells using an LDH redirected cell killing assay essentially as described below. The results of this investigation are shown in  FIGS. 16A-16B .
       

       FIGS. 16A-16B  show that DART-X (having an hCD16-M1 CD16 Binding Domain) exhibited a higher percentage cytotoxicity than DART-Y (having an hCD16-M2 CD16 Binding Domain). DART-0 (having an h3G8 CD16 Binding Domain) was found to mediate a higher level of cytotoxicity than DART-Y, but this effect was dependent on the effector cells having a 158V allotype ( FIG. 16A  vs.  FIG. 16B ). In contrast, the cytotoxicity mediated by DART-X or DART-Y was independent of CD16A allotype ( FIG. 16A  vs.  FIG. 16B ). 
     Example 8 
     Optimization of Binding to Non-Human Primate CD16 
     As noted above, hCD16-M1 was found to have low affinity to cynomolgus monkey CD16 (cynoCD16). Random mutagenesis was used to introduce substitutions within the CDR L 3 (Kabat positions 90-95) and CDR H 3 (Kabat positions 96-100) Domains of hCD16-M1. The mutants were screened to identify clones having enhanced binding to non-human primate CD16 (e.g., cynoCD16) and that retained binding affinity binding to both alleles of human CD16. A variant designated “hCD16-M1A” having a mutated CDR H 3, and a variant designated “hCD16-M1B” having a mutated CDR L 3 were selected for further analysis. In addition, a third variant combining the CDR H 3 and CDR L 3 mutations was generated and designated “hCD16-M1AB.” The amino acid sequence of the CDRs and VH and VL Domains have been provided above (see, e.g., Table 8). Exemplary CD16 x DA Binding Molecules incorporating the optimized anti-CD16 binding domains: hCD16-M1A, hCD16-M1B, or hCD16-M1AB, and having an anti-HER2/neu Binding Domain were generated and designated DART-I, DART-J, and DART-K, respectively (see, Table 12 for summary and above for detailed description and full amino acid sequences). 
     The ability of the exemplary CD16 x HER2/neu Binding Molecules comprising the optimized hCD16-M1A, hCD16-M1B, and hCD16-M1AB, to bind CD16 expressed on the surface of human ( FIG. 17A ), cynomolgus monkey ( FIG. 17B ), and rhesus monkey ( FIG. 17C ) NK cells was examined by flow cytometry. Briefly, PBMCs were isolated and incubated in the presence of CD16-binding diabodies: DART-C (having the parental hCD16-M1 VH and VL domains); DART-I; DART-J; DART-K (having the optimized hCD16-M1A, hCD16-M1B, and hCD16-M1AB VH/VL Domains, respectively); a HER2/neu x RSV diabody (as a negative control for CD16 binding); DART-3 (a CD16 x RSV diabody control having the having the parental hCD16-M1 VH and VL domains); or the DART-1 (h3G8 x HER2/neu diabody) comparator molecule. Following incubation, the cells were labeled with: anti-CD56-allophycocyanin (CD56 APC, NK cell marker), and anti-CD3-peridinin chlorophyll protein-Cy5.5 (CD3 PerCP Cy5.5, T-cell marker), and anti-human Fc-Phycoerythrin (αhFc PE) to detect diabody binding and the cell surface. The labeled cells were analyzed by flow cytometry gated on NK cells. 
     The co-staining results of the investigation are shown in  FIGS. 17A-17C  and indicate that the CD16 x DA Binding Molecules comprising the optimized variants, particularly hCD16-M1AB (DART-K), exhibited improved affinity for non-human primate CD16 ( FIGS. 17B-17C ), although molecules comprising hCD16-M1B (DART-J) and hCD16-M1AB (DART-K) exhibited some reduction in binding to human CD16 ( FIG. 17A ) in this assay. No binding was observed by the negative control. 
     The ability of CD16 x DA Binding Molecules having optimized hCD16-M1A, hCD16-M1B, and hCD16-M1AB binding domains, to mediate redirected cell killing of JIMT-1-Luc target cells with human PBMCs (huPBMCs) or cynomolgus monkey PBMC (cynoPBMCs) effector cells from several donors was evaluated using two different redirected cell killing assays. Representative data from these studies are presented in  FIGS. 18A-18D  and summarized in Table 18. In both assays, DART-C (having the parental hCD16-M1 VH and VL domains), DART-I, DART-J, or DART-K (having the optimized hCD16-M1A, hCD16-M1B, and hCD16-M1AB VH/VL Domains, respectively); a negative control (either HER2/neu x RSV diabody or the CD16 x RSV diabody, DART-3); or DART-1 (h3G8 x HER2/neu diabody) comparator molecule, were incubated with huPMBCs ( FIG. 18A-18B ) or cynoPBMCs ( FIGS. 18C-18D ) and JIMT-1-Luc target tumor cells at an E:T ration of 30:1 and the percentage cytotoxicity (i.e., cell killing) was determined. In one assay cell killing was determined by measuring the release of lactate dehydrogenase (LDH) into the media by damaged cells using an LDH redirected cell killing assay essentially as described below ( FIGS. 18A and 18C )). In another assay cytotoxicity was determined by luminescence (LUM) assay measuring cellular luciferase activity of the target cells using a LUM redirected cell killing assay essentially as described below ( FIGS. 18B and 18D ). The EC50 values (ng/mL) for the LDH assay after 24 hour and 48 hour incubation is presented in Table 18. 
     
       
         
           
               
               
               
             
               
                 TABLE 18 
               
             
            
               
                   
               
               
                   
                 24 hour Assay/LDH 
                 48 hour Assay/LDH 
               
               
                   
                 EC50, ng/mL 
                 EC50, ng/mL 
               
            
           
           
               
               
               
               
               
            
               
                   
                 HuPBMCs 
                 CynoPBMCs 
                 HuPBMCs 
                 CynoPBMCs 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 DART-1 
                 131 
                 170 
                 156.1 
                 101 
               
               
                 DART-C 
                 1.42 
                 51.47 
                 0.822 
                 70.6 
               
               
                 DART-I 
                 1.05 
                 36.98 
                 0.492 
                 13.31 
               
               
                 DART-J 
                 4.4 
                 39.91 
                 1.06 
                 10.88 
               
               
                 DART-K 
                 10.8 
                 29.44 
                 2.24 
                 6.66 
               
               
                   
               
            
           
         
       
     
     These data show that, although the CD16 x DA Binding Molecule comprising the CD16 binding domain of hCD16-M1 (DART-C) binds cynoCD16 with apparent low affinity, it is capable of mediating redirected cell killing. In addition, CD16 x DA Binding Molecules comprising the optimized CD16 binding domains hCD16-M1A (DART-I), hCD16-M1B (DART-J), or hCD16-M1AB (DART-K) exhibited improved cytotoxicity with CynoPBMCs while exhibiting only a slight reduction in cytotoxicity with huPBMCs as compared to the same molecule comprising hCD16-M1 (DART-C). 
     Example 9 
     CD16 x CD19 Binding Molecules 
     In further studies, exemplary CD16 x DA Binding Molecules having an anti-CD19 Binding Domain and incorporating the anti-CD16 binding domain of hCD16-M1, or the optimized anti-CD16 binding domain of hCD16-M1B or hCD16-M1AB, were generated and designated DART-L, DART-M, and DART-N, respectively (see, Table 12 for summary and above for detailed description and full amino acid sequences). Three additional molecules were generated and designated DART-5 (comprising hCD16-M1), DART-6 (comprising hCD16-M1A), and DART-7 (comprising hCD16-M1AB), in which the CD19 Binding Domain was replaced with an anti-RSV binding domain (see, Table 12 for summary and above for detailed description), such exemplary CD16 x RSV Binding Molecules are used below as negative controls for CD19 binding. 
     The ability of: DART-L, DART-M, and DART-N; the control molecules: DART-5, DART-6, and DART-7; or the CD3 x CD19 DART® diabody duvortuxizumab (also known as MGD011; amino acid sequence found in WHO Drug Information, 2016, Proposed INN: List 116, 30(4):627-629) to mediate redirected cell killing of Raji-Luc target cells with human PBMCs (huPBMCs) effector cells (E:T=30:1) was evaluated in the LDH and LUM cell killing assays essentially as described below. Representative data from these studies are presented in  FIGS. 19A-19D . The results of the LDH assays show that after 24 hours ( FIG. 19A ) and 48 hour ( FIG. 19B ) incubations the CD16 x DA Binding Molecules exhibited similar cytotoxicity activity as MGD011, with the CD16 x DA Binding Molecules comprising the optimized CD16 binding domains hCD16-M1B (DART-M), or hCD16-M1AB (DART-N), exhibiting similar cytotoxicity with huPBMCs as compared to the same molecule comprising hCD16-M1 (DART-L). Similar results were observed after 24 hours ( FIG. 19C ) and 48 hours ( FIG. 19D ) in the LUM assays. Minimal cytotoxicity was observed for the control molecules lacking a CD19 binding domain (DART-5, DART-6, and DART-7) in these studies. 
     The exemplary CD16 x DA Binding Molecules DART-L, DART-M, DART-N (having a binding site for the B-cell antigen CD19) and the negative controls: DART-5 and DART-6 (having a binding site for CD16 and a binding site for RSV) were evaluated for their ability to mediate autologous B-cell depletion in vitro. Briefly, PMBCs isolated from human or cynomolgus monkey were incubated in supplemented medium in the presence of increasing concentrations DART-L, DART-M, DART-N and the control molecules lacking a CD19 binding domain DART-5 and DART-6. B-cell levels were analyzed by flow cytometry (using CD3 for negative selection and CD20 as a B-cell marker; CD3/CD20 + ) at 72 hours and 96 hours post incubation for huPBMCs ( FIGS. 20A-20B , respectively) and at 72 hours and 144 hours post incubation for cynoPBMCs ( FIGS. 20C-20D , respectively). Representative data from these studies are presented in  FIGS. 20A-20D , and show that all the CD16 x DA Binding Molecules were able to deplete autologous B-cells from both huPBMCs ( FIGS. 20A-20B ) and cynoPBMCs ( FIGS. 20C-20D ). 
     CD16 x DA Binding Molecules comprising the optimized CD16 binding domains hCD16-M1B (DART-M), or hCD16-M1AB (DART-N), mediated a larger reduction in B-cells, with both huPBMCs and cynoPBMCs as compared to the same molecule comprising hCD16-M1 (DART-L). However, higher concentrations were required to deplete B-cells from cynoPBMCs. This trend was observed for multiple PBMC donors. Minimal B-cell depletion was observed for the control molecules lacking a CD19 binding domain (DART-5 and DART-6). 
     Example 10 
     Exemplary Redirected Cell Killing Assays 
     LDH redirected cell killing assay: These assays may be performed using the CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (Promega), or similar, that quantitatively measures LDH release essentially as described below. Target cells (e.g., tumor target cells) are resuspended at a density of 4×10 5  cells/mL (or appropriate density to achieve the desired E:T ratio) in assay media (e.g., RPMI 1640 without phenol red, 10% FBS, 1% pen/strep) and preferably a viability of higher than 90% at assay initiation, and isolated purified effector cells (e.g., PBMC or NK cells purified from human or non-human primate (e.g., cynomolgus monkey) donor) suspended in the assay media at the appropriate density (e.g., 4×10 5  cells/mL) to achieve an effector-to-target (E:T) cell ratio of 10:1, or 30:1 (or other desired E:T ratio) are used. An aliquot of target cell suspension (e.g., 50 μL, 20,000 cells), an aliquot of effector cell suspension (e.g., 100 μL, 200,000 cells for 10:1 E:T ratio), and an aliquot (e.g., 50 μL) of serially diluted test article (e.g., 5 fold, or 10 fold) are added to duplicate wells of a microtiter plate and incubated (37° C. with 5% CO2) for 24-96 hours, or longer if desired. At the end of the incubation an aliquot of lysis solution (e.g., 30 μL) is added and the plates are incubated for ˜10 minutes to completely lyse the target cells. The plates are then centrifuged to pellet the cell debris (e.g., 212 x g for 5 minutes) and an aliquot (e.g., 40 μL) of supernatant of is transferred from each well of the assay plate to a flat-bottom ELISA plate and an aliquot (e.g., 40 μL) of LDH substrate solution is added to each well. Plates are incubated for 10-20 minutes at room temperature in the dark and an aliquot (e.g., 40 μL) of stop solution (Promega Cat #G183A) is added. The optical density is measured at 490 nm within 1 hour on a Victor2 Multilabel plate reader (Perkin Elmer #1420-014), or similar. Specific cell lysis is calculated from optical density (OD) data using the following formula: 
     
       
         
           
             
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     wherein “AICC” is antibody-independent cellular cytotoxicity, “MR” is maximal release and “SR” is spontaneous release. The dose-response curves are generated using GraphPad Prism 6 software (or similar) by curve fitting the cytotoxicity values to the sigmoidal dose-response function. 
     Luminescence (LUM) redirected cell killing assay: These assays may be performed using the Steady-Glo luciferase substrate (Promega), or a similar sustrate, and quantitatively measure celluar luciferase activity in living target cells engineered to express the luciferase (luc) reporter gene (e.g., JIMT-1-Luc, Raji-Luc cells) essentially as described below. The preparation and set up for these assays is essentially identical to the LDH assay described above. Following incubation, an aliquot (e.g., 100 μL) of culture medium is removed from each well and an aliquot (e.g., 100 μL) of Steady-Glo luciferase substrate ((Promega), or similar) is subsequently added to each well, followed by pipetting up/down several times for complete lysis of target cells. The plates are incubated at room temperature in the dark for 10 minutes and then luminescence intensity is measured using a VictorX4 Multilabel plate reader (Perkin Elmer #1420-014, or similar) with luminescence relative light unit (RLU) as the read-out. RLU is indicative of relative viability of the target cells. Dose-response curves are generated using GraphPad Prism 6 software (or similar) by curve fitting the RLU values to the sigmoidal dose-response function. 
     All publications and patents mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference in its entirety. While the invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications and this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains and as may be applied to the essential features hereinbefore set forth.