Patent Publication Number: US-2005137129-A1

Title: Novel compounds

Description:
This application is a continuation-in-part of application Ser. No. 10/312,088 filed Dec. 20, 2002, (now pending) which is the National Stage of International Application No. PCT/US01/19929, filed Jun. 22, 2001, which claims the benefit of Provisional Application No. 60/213,161, filed Jun. 22, 2000, and Provisional Application No. 60/213,156, filed Jun. 22, 2000. 
    
    
     FIELD OF INVENTION  
      This invention relates to newly identified polypeptides and polynucleotides encoding such polypeptides, to their use in diagnosis and in identifying compounds that may be agonists, antagonists that are potentially useful in therapy, and to production of such polypeptides and polynucleotides. The polynucleotides and polypeptides of the present invention also relate to proteins with signal sequences which allow them to be secreted extracellularly or membrane-associated (hereinafter often referred collectively as secreted proteins or secreted polypeptides).  
     BACKGROUND OF THE INVENTION  
      The drug discovery process is currently undergoing a fundamental revolution as it embraces “functional genomics”, that is, high throughput genome- or gene-based biology. This approach as a means to identify genes and gene products as therapeutic targets is rapidly superseding earlier approaches based on “positional cloning”. A phenotype, that is a biological function or genetic disease, would be identified and this would then be tracked back to the responsible gene, based on its genetic map position.  
      Functional genomics relies heavily on high-throughput DNA sequencing technologies and the various tools of bioinformatics to identify gene sequences of potential interest from the many molecular biology databases now available. There is a continuing need to identify and characterise further genes and their related polypeptides/proteins, as targets for drug discovery.  
      Proteins and polypeptides that are naturally secreted into blood, lymph and other body fluids, or secreted into the cellular membrane are of primary interest for research and development of protein therapeutic agents. The reason for this interest is the relative ease to target secreted protein therapeutics into their place of action (body fluids or the cellular membrane). Secreted proteins, and the extracellular regions of transmembrane proteins, can be directly administered into body fluids, or can be directed to body fluids or membranes by a natural pathway. The natural pathway for protein secretion into extracellular space is the endoplasmic reticulum in eukaryotes and the inner membrane in prokaryotes (Palade, 1975, Science, 189, 347; Milstein, Brownlee, Harrison, and Mathews, 1972, Nature New Biol., 239, 117; Blobel, and Dobberstein, 1975, J. Cell. Biol., 67, 835). On the other hand, there is no known natural pathway for exporting a protein from the exterior of the cells into the cytosol (with the exception of pinocytosis, a mechanism of snake venom toxin intrusion into cells). Therefore targeting protein therapeutics into cells poses extreme difficulties.  
      The secreted and membrane-associated proteins include but are not limited to all peptide hormones and their receptors (including but not limited to insulin, growth hormones, chemokines, cytokines, colony-stimulating factors such as enythropoietin, neuropeptides, integrins, kallikreins, lamins, melanins, natriuretic hormones, neuropsin, neurotropins, pituitiary hormones, pleiotropins, prostaglandins, secretogranins, selectins, thromboglobulins, thymosins), cytokine receptors, the breast and colon cancer gene products, the obesity gene protein leptin and its receptors, serum albumin, superoxide dismutase, spliceosome proteins, 7TM (transmembrane) proteins also called as G-protein coupled receptors, immunoglobulins, several families of serine proteinases (including but not limited to proteins of the blood coagulation cascade, digestive enzymes), deoxyribonuclease I, etc.  
      Therapeutics based on secreted or membrane-associated proteins approved by FDA or foreign agencies include but are not limited to insulin, glucagon, growth hormone, chorionic gonadotropin, follicle stimulating hormone, luteinizing hormone, calcitonin, adrenocorticotropic hormone (ACTH), vasopressin, interleukines, interferons, erythropoietin, the extracellular region of the transmembrane cytokine receptor TNFalpha (soluble TNFalpha receptor or Enbrel), immunoglobulins, lactoferrin (diverse products marketed by several companies), tissue-type plasminogen activator (Alteplase by Genentech), hyaulorindase (Wydase by Wyeth-Ayerst), dornase alpha (Pulmozyme\ by Genentech), Chymodiactin (chymopapain by Knoll), alglucerase (Ceredase by Genzyme), streptokinase (Kabikinase by Pharmacia) (Streptase by Astra), etc. This indicates that secreted and membrane-associated proteins have an established, proven history as therapeutic targets. Clearly, there is a need for identification and characterization of further secreted and membrane-associated proteins which can play a role in preventing, ameliorating or correcting dysfunction or disease, including but not limited to diabetes, breast-, prostate-, colon cancer and other malignant tumors, hyper- and hypotension, obesity, bulimia, anorexia, growth abnormalities, asthma, manic depression, dementia, delirium, mental retardation, Huntington&#39;s disease, Tourette&#39;s syndrome, schizophrenia, growth, mental or sexual development disorders, and dysfunctions of the blood cascade system including those leading to stroke. The proteins of the present invention which include the signal sequences are also useful to further elucidate the mechanism of protein transport which at present is not entirely understood, and thus can be used as research tools. SUMMARY OF THE INVENTION  
      The present invention relates to particular polypeptides and polynucleotides of the genes set forth in Table I, including recombinant materials and methods for their production. Such polypeptides and polynucleotides are of interest in relation to methods of treatment of certain diseases, including, but not limited to, the diseases set forth in Tables III and V, hereinafter referred to as “diseases of the invention”. In a further aspect, the invention relates to methods for identifying agonists and antagonists (e.g., inhibitors) using the materials provided by the invention, and treating conditions associated with imbalance of polypeptides and/or polynucleotides of the genes set forth in Table I with the identified compounds. In still a further aspect, the invention relates to diagnostic assays for detecting diseases associated with inappropriate activity or levels the genes set forth in Table I. Another aspect of the invention concerns a polynucleotide comprising any of the nucleotide sequences set forth in the Sequence Listing and a polypeptide comprising a polypeptide encoded by the nucleotide sequence. In another aspect, the invention relates to a polypeptide comprising any of the polypeptide sequences set forth in the Sequence Listing and recombinant materials and methods for their production. Another aspect of the invention relates to methods for using such polypeptides and polynucleotides. Such uses include the treatment of diseases, abnormalities and disorders (hereinafter simply referred to as diseases) caused by abnormal expression, production, function and or metabolism of the genes of this invention, and such diseases are readily apparent by those skilled in the art from the homology to other proteins disclosed for each attached sequence. In still another aspect, the invention relates to methods to identify agonists and antagonists using the materials provided by the invention, and treating conditions associated with the imbalance with the identified compounds. Yet another aspect of the invention relates to diagnostic assays for detecting diseases associated with inappropriate activity or levels of the secreted proteins of the present invention. 
    
    
     BRIEF DESCRIPTION OF THE FIGURE  
       FIG. 1  demonstrates enhancement of would closure in ob/ob mice by administration of an adenovirus encoding in its genome β-Tectorin. 
    
    
     DESCRIPTION OF THE INVENTION  
      In a first aspect, the present invention relates to polypeptides the genes set forth in Table I. Such polypeptides include: 
          (a) an isolated polypeptide encoded by a polynucleotide comprising a sequence set forth in the Sequence Listing;     (b) an isolated polypeptide comprising a polypeptide sequence having at least 95%, 96%, 97%, 98%, or 99% identity to a polypeptide sequence set forth in the Sequence Listing;     (c) an isolated polypeptide comprising a polypeptide sequence set forth in the Sequence Listing;     (d) an isolated polypeptide having at least 95%, 96%, 97%, 98%, or 99% identity to a polypeptide sequence set forth in the Sequence Listing;     (e) a polypeptide sequence set forth in the Sequence Listing; and     (f) an isolated polypeptide having or comprising a polypeptide sequence that has an Identity Index of 0.95, 0.96, 0.97, 0.98, or 0.99 compared to a polypeptide sequence set forth in the Sequence Listing;     (g) fragments and variants of such polypeptides in (a) to (f). 
 
 Polypeptides of the present invention are believed to be members of the gene families set forth in Table II. They are therefore of therapeutic and diagnostic interest for the reasons set forth in Tables III and V. The biological properties of the polypeptides and polynucleotides of the genes set forth in Table I are hereinafter referred to as “the biological activity” of polypeptides and polynucleotides of the genes set forth in Table I. Preferably, a polypeptide of the present invention exhibits at least one biological activity of the genes set forth in Table I. 
       

      Polypeptides of the present invention also include variants of the aforementioned polypeptides, including all allelic forms and splice variants. Such polypeptides vary from the reference polypeptide by insertions, deletions, and substitutions that may be conservative or non-conservative, or any combination thereof. Particularly preferred variants are those in which several, for instance from 50 to 30, from 30 to 20, from 20 to 10, from 10 to 5, from 5 to 3, from 3 to 2, from 2 to 1 or 1 amino acids are inserted, substituted, or deleted, in any combination.  
      Preferred fragments of polypeptides of the present invention include an isolated polypeptide comprising an amino acid sequence having at least 30, 50 or 100 contiguous amino acids from an amino acid sequence set forth in the Sequence Listing, or an isolated polypeptide comprising an amino acid sequence having at least 30, 50 or 100 contiguous amino acids truncated or deleted from an amino acid sequence set forth in the Sequence Listing. Preferred fragments are biologically active fragments that mediate the biological activity of polypeptides and polynucleotides of the genes set forth in Table I, including those with a similar activity or an improved activity, or with a decreased undesirable activity. Also preferred are those fragments that are antigenic or immunogenic in an animal, especially in a human.  
      Fragments of a polypeptide of the invention may be employed for producing the corresponding full-length polypeptide by peptide synthesis; therefore, these variants may be employed as intermediates for producing the full-length polypeptides of the invention. A polypeptide of the present invention may be in the form of the “mature” protein or may be a part of a larger protein such as a precursor or a fusion protein. It is often advantageous to include an additional amino acid sequence that contains secretory or leader sequences, pro-sequences, sequences that aid in purification, for instance multiple histidine residues, or an additional sequence for stability during recombinant production.  
      Polypeptides of the present invention can be prepared in any suitable manner, for instance by isolation from naturally occurring sources, from genetically engineered host cells comprising expression systems (vide infra) or by chemical synthesis, using for instance automated peptide synthesizers, or a combination of such methods. Means for preparing such polypeptides are well understood in the art.  
      In a further aspect, the present invention relates to polynucleotides of the genes set forth in Table I. Such polynucleotides include: 
          (a) an isolated polynucleotide comprising a polynucleotide sequence having at least 95%, 96%, 97%, 98%, or 99% identity to a polynucleotide sequence set forth in the Sequence Listing;     (b) an isolated polynucleotide comprising a polynucleotide set forth in the Sequence Listing;     (c) an isolated polynucleotide having at least 95%, 96%, 97%, 98%, or 99% identity to a polynucleotide set forth in the Sequence Listing;     (d) an isolated polynucleotide set forth in the Sequence Listing;     (e) an isolated polynucleotide comprising a polynucleotide sequence encoding a polypeptide sequence having at least 95%, 96%, 97%, 98%, or 99% identity to a polypeptide sequence set forth in the Sequence Listing;     (f) an isolated polynucleotide comprising a polynucleotide sequence encoding a polypeptide set forth in the Sequence Listing;     (g) an isolated polynucleotide having a polynucleotide sequence encoding a polypeptide sequence having at least 95%, 96%, 97%, 98%, or 99% identity to a polypeptide sequence set forth in the Sequence Listing;     (h) an isolated polynucleotide encoding a polypeptide set forth in the Sequence Listing;     (i) an isolated polynucleotide having or comprising a polynucleotide sequence that has an Identity Index of 0.95, 0.96, 0.97, 0.98, or 0.99 compared to a polynucleotide sequence set forth in the Sequence Listing;     (j) an isolated polynucleotide having or comprising a polynucleotide sequence encoding a polypeptide sequence that has an Identity Index of 0.95, 0.96, 0.97, 0.98, or 0.99 compared to a polypeptide sequence set forth in the Sequence Listing; and     polynucleotides that are fragments and variants of the above mentioned polynucleotides or that are complementary to above mentioned polynucleotides, over the entire length thereof.        

      Preferred fragments of polynucleotides of the present invention include an isolated polynucleotide comprising an nucleotide sequence having at least 15, 30, 50 or 100 contiguous nucleotides from a sequence set forth in the Sequence Listing, or an isolated polynucleotide comprising a sequence having at least 30, 50 or 100 contiguous nucleotides truncated or deleted from a sequence set forth in the Sequence Listing.  
      Preferred variants of polynucleotides of the present invention include splice variants, allelic variants, and polymorphisms, including polynucleotides having one or more single nucleotide polymorphisms (SNPs).  
      Polynucleotides of the present invention also include polynucleotides encoding polypeptide variants that comprise an amino acid sequence set forth in the Sequence Listing and in which several, for instance from 50 to 30, from 30 to 20, from 20 to 10, from 10 to 5, from 5 to 3, from 3 to 2, from 2 to 1 or 1 amino acid residues are substituted, deleted or added, in any combination. In a further aspect, the present invention provides polynucleotides that are RNA transcripts of the DNA sequences of the present invention. Accordingly, there is provided an RNA polynucleotide that: 
          (a) comprises an RNA transcript of the DNA sequence encoding a polypeptide set forth in the Sequence Listing;     (b) is a RNA transcript of a DNA sequence encoding a polypeptide set forth in the Sequence Listing;     (c) comprises an RNA transcript of a DNA sequence set forth in the Sequence Listing; or     (d) is a RNA transcript of a DNA sequence set forth in the Sequence Listing; and RNA polynucleotides that are complementary thereto.        

      The polynucleotide sequences set forth in the Sequence Listing show homology with the polynucleotide sequences set forth in Table II. A polynucleotide sequence set forth in the Sequence Listing is a cDNA sequence that encodes a polypeptide set forth in the Sequence Listing. A polynucleotide sequence encoding a polypeptide set forth in the Sequence Listing may be identical to a polypeptide encoding a sequence set forth in the Sequence Listing or it may be a sequence other than a sequence set forth in the Sequence Listing, which, as a result of the redundancy (degeneracy) of the genetic code, also encodes a polypeptide set forth in the Sequence Listing. A polypeptide of a sequence set forth in the Sequence Listing is related to other proteins of the gene families set forth in Table II, having homology and/or structural similarity with the polypeptides set forth in Table II. Preferred polypeptides and polynucleotides of the present invention are expected to have, inter alia, similar biological functions/properties to their homologous polypeptides and polynucleotides. Furthermore, preferred polypeptides and polynucleotides of the present invention have at least one activity of the genes set forth in Table I.  
      Polynucleotides of the present invention may be obtained using standard cloning and screening techniques from a cDNA library derived from mRNA from the tissues set forth in Table IV (see for instance, Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989)). Polynucleotides of the invention can also be obtained from natural sources such as genomic DNA libraries or can be synthesized using well known and commercially available techniques.  
      When polynucleotides of the present invention are used for the recombinant production of polypeptides of the present invention, the polynucleotide may include the coding sequence for the mature polypeptide, by itself, or the coding sequence for the mature polypeptide in reading frame with other coding sequences, such as those encoding a leader or secretory sequence, a pre-, or pro- or prepro- protein sequence, or other fusion peptide portions. For example, a marker sequence that facilitates purification of the fused polypeptide can be encoded. In certain preferred embodiments of this aspect of the invention, the marker sequence is a hexa-histidine peptide, as provided in the pQE vector (Qiagen, Inc.) and described in Gentz et al., Proc Natl Acad Sci USA (1989) 86:821-824, or is an HA tag. A polynucleotide may also contain non-coding 5′ and 3′ sequences, such as transcribed, non-translated sequences, splicing and polyadenylation signals, ribosome binding sites and sequences that stabilize mRNA.  
      Polynucleotides that are identical, or have sufficient identity to a polynucleotide sequence set forth in the Sequence Listing, may be used as hybridization probes for cDNA and genomic DNA or as primers for a nucleic acid amplification reaction (for instance, PCR). Such probes and primers may be used to isolate full-length cDNAs and genomic clones encoding polypeptides of the present invention and to isolate cDNA and genomic clones of other genes (including genes encoding paralogs from human sources and orthologs and paralogs from other species) that have a high sequence similarity to sequences set forth in the Sequence Listing, typically at least 95% identity. Preferred probes and primers will generally comprise at least 15 nucleotides, preferably, at least 30 nucleotides and may have at least 50, if not at least 100 nucleotides. Particularly preferred probes will have between 30 and 50 nucleotides. Particularly preferred primers will have between 20 and 25 nucleotides.  
      A polynucleotide encoding a polypeptide of the present invention, including homologs from other species, may be obtained by a process comprising the steps of screening a library under stringent hybridization conditions with a labeled probe having a sequence set forth in the Sequence Listing or a fragment thereof, preferably of at least 15 nucleotides; and isolating full-length cDNA and genomic clones containing the polynucleotide sequence set forth in the Sequence Listing. Such hybridization techniques are well known to the skilled artisan. Preferred stringent hybridization conditions include overnight incubation at 42° C. in a solution comprising: 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5× Denhardt&#39;s solution, 10 % dextran sulfate, and 20 microgram/ml denatured, sheared salmon sperm DNA; followed by washing the filters in 0.1×SSC at about 65° C. Thus the present invention also includes isolated polynucleotides, preferably with a nucleotide sequence of at least 100, obtained by screening a library under stringent hybridization conditions with a labeled probe having the sequence set forth in the Sequence Listing or a fragment thereof, preferably of at least 15 nucleotides.  
      The skilled artisan will appreciate that, in many cases, an isolated cDNA sequence will be incomplete, in that the region coding for the polypeptide does not extend all the way through to the 5′ terminus. This is a consequence of reverse transcriptase, an enzyme with inherently low “processivity” (a measure of the ability of the enzyme to remain attached to the template during the polymerisation reaction), failing to complete a DNA copy of the mRNA template during first strand cDNA synthesis.  
      There are several methods available and well known to those skilled in the art to obtain full-length cDNAs, or extend short cDNAs, for example those based on the method of Rapid Amplification of cDNA ends (RACE) (see, for example, Frohman et al., Proc Nat Acad Sci USA 85, 8998-9002, 1988). Recent modifications of the technique, exemplified by the Marathon (trade mark) technology (Clontech Laboratories Inc.) for example, have significantly simplified the search for longer cDNAs. In the Marathon (trade mark) technology, cDNAs have been prepared from mRNA extracted from a chosen tissue and an ‘adaptor’ sequence ligated onto each end. Nucleic acid amplification (PCR) is then carried out to amplify the “missing” 5′ end of the cDNA using a combination of gene specific and adaptor specific oligonucleotide primers. The PCR reaction is then repeated using ‘nested’ primers, that is, primers designed to anneal within the amplified product (typically an adapter specific primer that anneals further 3′ in the adaptor sequence and a gene specific primer that anneals further 5′ in the known gene sequence). The products of this reaction can then be analyzed by DNA sequencing and a full-length cDNA constructed either by joining the product directly to the existing cDNA to give a complete sequence, or carrying out a separate full-length PCR using the new sequence information for the design of the 5′ primer.  
      Recombinant polypeptides of the present invention may be prepared by processes well known in the art from genetically engineered host cells comprising expression systems. Accordingly, in a further aspect, the present invention relates to expression systems comprising a polynucleotide or polynucleotides of the present invention, to host cells which are genetically engineered with such expression systems and to the production of polypeptides of the invention by recombinant techniques. Cell-free translation systems can also be employed to produce such proteins using RNAs derived from the DNA constructs of the present invention.  
      For recombinant production, host cells can be genetically engineered to incorporate expression systems or portions thereof for polynucleotides of the present invention. Polynucleotides may be introduced into host cells by methods described in many standard laboratory manuals, such as Davis et al., Basic Methods in Molecular Biology (1986) and Sambrook et al.(ibid). Preferred methods of introducing polynucleotides into host cells include, for instance, calcium phosphate transfection, DEAE-dextran mediated transfection, transvection, micro-injection, cationic lipid-mediated transfection, electroporation, transduction, scrape loading, ballistic introduction or infection.  
      Representative examples of appropriate hosts include bacterial cells, such as  Streptococci, Staphylococci, E. coli, Streptomyces  and  Bacillus subtilis  cells; fungal cells, such as yeast cells and  Aspergillus  cells; insect cells such as  Drosophila  S2 and  Spodoptera  Sf9 cells; animal cells such as CHO, COS, HeLa, C127, 3T3, BHK, HEK 293 and Bowes melanoma cells; and plant cells.  
      A great variety of expression systems can be used, for instance, chromosomal, episomal and virus-derived systems, e.g., vectors derived from bacterial plasmids, from bacteriophage, from transposons, from yeast episomes, from insertion elements, from yeast chromosomal elements, from viruses such as baculoviruses, papova viruses, such as SV40, vaccinia viruses, adenoviruses, adeno-associated viruses, fowl pox viruses, pseudorabies viruses and retroviruses, and vectors derived from combinations thereof, such as those derived from plasmid and bacteriophage genetic elements, such as cosmids and phagemids. The expression systems may contain control regions that regulate as well as engender expression. Generally, any system or vector that is able to maintain, propagate or express a polynucleotide to produce a polypeptide in a host may be used. The appropriate polynucleotide sequence may be inserted into an expression system by any of a variety of well-known and routine techniques, such as, for example, those set forth in Sambrook et al., (ibid). Appropriate secretion signals may be incorporated into the desired polypeptide to allow secretion of the translated protein into the lumen of the endoplasmic reticulum, the periplasmic space or the extracellular environment. These signals may be endogenous to the polypeptide or they may be heterologous signals.  
      If a polypeptide of the present invention is to be expressed for use in screening assays, it is generally preferred that the polypeptide be produced at the surface of the cell. In this event, the cells may be harvested prior to use in the screening assay. If the polypeptide is secreted into the medium, the medium can be recovered in order to recover and purify the polypeptide. If produced intracellularly, the cells must first be lysed before the polypeptide is recovered.  
      Polypeptides of the present invention can be recovered and purified from recombinant cell cultures by well-known methods including ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite chromatography and lectin chromatography. Most preferably, high performance liquid chromatography is employed for purification. Well known techniques for refolding proteins may be employed to regenerate active conformation when the polypeptide is denatured during intracellular synthesis, isolation and/or purification.  
      Polynucleotides of the present invention may be used as diagnostic reagents, through detecting mutations in the associated gene. Detection of a mutated form of a gene is characterized by the polynucleotides set forth in the Sequence Listing in the cDNA or genomic sequence and which is associated with a dysfunction. Will provide a diagnostic tool that can add to, or define, a diagnosis of a disease, or susceptibility to a disease, which results from under-expression, over-expression or altered spatial or temporal expression of the gene. Individuals carrying mutations in the gene may be detected at the DNA level by a variety of techniques well known in the art.  
      Nucleic acids for diagnosis may be obtained from a subject&#39;s cells, such as from blood, urine, saliva, tissue biopsy or autopsy material. The genomic DNA may be used directly for detection or it may be amplified enzymatically by using PCR, preferably RT-PCR, or other amplification techniques prior to analysis. RNA or cDNA may also be used in similar fashion. Deletions and insertions can be detected by a change in size of the amplified product in comparison to the normal genotype. Point mutations can be identified by hybridizing amplified DNA to labeled nucleotide sequences of the genes set forth in Table I. Perfectly matched sequences can be distinguished from mismatched duplexes by RNase digestion or by differences in melting temperatures. DNA sequence difference may also be detected by alterations in the electrophoretic mobility of DNA fragments in gels, with or without denaturing agents, or by direct DNA sequencing (see, for instance, Myers et al, Science (1985) 230:1242). Sequence changes at specific locations may also be revealed by nuclease protection assays, such as RNase and SI protection or the chemical cleavage method (see Cotton et al., Proc Natl Acad Sci USA (1985) 85: 4397-4401).  
      An array of oligonucleotides probes comprising polynucleotide sequences or fragments thereof of the genes set forth in Table I can be constructed to conduct efficient screening of e.g., genetic mutations. Such arrays are preferably high density arrays or grids. Array technology methods are well known and have general applicability and can be used to address a variety of questions in molecular genetics including gene expression, genetic linkage, and genetic variability, see, for example, M. Chee et al., Science, 274, 610-613 (1996) and other references cited therein. Detection of abnormally decreased or increased levels of polypeptide or mRNA expression may also be used for diagnosing or determining susceptibility of a subject to a disease of the invention. Decreased or increased expression can be measured at the RNA level using any of the methods well known in the art for the quantitation of polynucleotides, such as, for example, nucleic acid amplification, for instance PCR, RT-PCR, RNase protection, Northern blotting and other hybridization methods. Assay techniques that can be used to determine levels of a protein, such as a polypeptide of the present invention, in a sample derived from a host are well-known to those of skill in the art. Such assay methods include radio-immunoassays, competitive-binding assays, Western Blot analysis and ELISA assays.  
      Thus in another aspect, the present invention relates to a diagnostic kit comprising: 
          (a) a polynucleotide of the present invention, preferably the nucleotide sequence set forth in the Sequence Listing, or a fragment or an RNA transcript thereof;     (b) a nucleotide sequence complementary to that of (a);     (c) a polypeptide of the present invention, preferably the polypeptide set forth in the Sequence Listing or a fragment thereof; or     (d) an antibody to a polypeptide of the present invention, preferably to the polypeptide set forth in the Sequence Listing.        

      It will be appreciated that in any such kit, (a), (b), (c) or (d) may comprise a substantial component. Such a kit will be of use in diagnosing a disease or susceptibility to a disease, particularly diseases of the invention, amongst others.  
      The polynucleotide sequences of the present invention are valuable for chromosome localisation studies. The sequences set forth in the Sequence Listing are specifically targeted to, and can hybridize with, a particular location on an individual human chromosome. The mapping of relevant sequences to chromosomes according to the present invention is an important first step in correlating those sequences with gene associated disease. Once a sequence has been mapped to a precise chromosomal location, the physical position of the sequence on the chromosome can be correlated with genetic map data. Such data are found in, for example, V. McKusick, Mendelian Inheritance in Man (available on-line through Johns Hopkins University Welch Medical Library). The relationship between genes and diseases that have been mapped to the same chromosomal region are then identified through linkage analysis (co-inheritance of physically adjacent genes). Precise human chromosomal localisations for a genomic sequence (gene fragment etc.) can be determined using Radiation Hybrid (RH) Mapping (Walter, M. Spillett, D., Thomas, P., Weissenbach, J., and Goodfellow, P., (1994) A method for constructing radiation hybrid maps of whole genomes, Nature Genetics 7, 22-28). A number of RH panels are available from Research Genetics (Huntsville, Ala., USA) e.g. the GeneBridge4 RH panel (Hum Mol Genet 1996 Mar;5(3):339-46 A radiation hybrid map of the human genome. Gyapay G, Schmitt K, Fizames C, Jones H, Vega-Czarny N, Spillett D, Muselet D, Prud&#39;Homme J F, Dib C, Auffray C, Morissette J, Weissenbach J, Goodfellow P N). To determine the chromosomal location of a gene using this panel, 93 PCRs are performed using primers designed from the gene of interest on RH DNAs. Each of these DNAs contains random human genomic fragments maintained in a hamster background (human/hamster hybrid cell lines). These PCRs result in 93 scores indicating the presence or absence of the PCR product of the gene of interest. These scores are compared with scores created using PCR products from genomic sequences of known location. This comparison is conducted at http://www.genome.wi.mit.edu/.  
      The polynucleotide sequences of the present invention are also valuable tools for tissue expression studies. Such studies allow the determination of expression patterns of polynucleotides of the present invention which may give an indication as to the expression patterns of the encoded polypeptides in tissues, by detecting the mRNAs that encode them. The techniques used are well known in the art and include in situ hydridization techniques to clones arrayed on a grid, such as cDNA microarray hybridization (Schena et al, Science, 270, 467-470, 1995 and Shalon et al, Genome Res, 6, 639-645, 1996) and nucleotide amplification techniques such as PCR. A preferred method uses the TAQMAN (Trade mark) technology available from Perkin Elmer. Results from these studies can provide an indication of the normal function of the polypeptide in the organism. In addition, comparative studies of the normal expression pattern of mRNAs with that of mRNAs encoded by an alternative form of the same gene (for example, one having an alteration in polypeptide coding potential or a regulatory mutation) can provide valuable insights into the role of the polypeptides of the present invention, or that of inappropriate expression thereof in disease. Such inappropriate expression may be of a temporal, spatial or simply quantitative nature.  
      A further aspect of the present invention relates to antibodies. The polypeptides of the invention or their fragments, or cells expressing them, can be used as immunogens to produce antibodies that are immunospecific for polypeptides of the present invention. The term “immunospecific” means that the antibodies have substantially greater affinity for the polypeptides of the invention than their affinity for other related polypeptides in the prior art.  
      Antibodies generated against polypeptides of the present invention may be obtained by administering the polypeptides or epitope-bearing fragments, or cells to an animal, preferably a non-human animal, using routine protocols. For preparation of monoclonal antibodies, any technique which provides antibodies produced by continuous cell line cultures can be used. Examples include the hybridoma technique (Kohler, G. and Milstein, C., Nature (1975) 256:495-497), the trioma technique, the human B-cell hybridoma technique (Kozbor et al., Immunology Today (1983) 4:72) and the EBV-hybridoma technique (Cole et al., Monoclonal Antibodies and Cancer Therapy, 77-96, Alan R. Liss, Inc., 1985).  
      Techniques for the production of single chain antibodies, such as those described in U.S. Pat. No. 4,946,778, can also be adapted to produce single chain antibodies to polypeptides of this invention. Also, transgenic mice, or other organisms, including other mammals, may be used to express humanized antibodies.  
      The above-described antibodies may be employed to isolate or to identify clones expressing the polypeptide or to purify the polypeptides by affinity chromatography. Antibodies against polypeptides of the present invention may also be employed to treat diseases of the invention, amongst others.  
      Polypeptides and polynucleotides of the present invention may also be used as vaccines. Accordingly, in a further aspect, the present invention relates to a method for inducing an immunological response in a mammal that comprises inoculating the mammal with a polypeptide of the present invention, adequate to produce antibody and/or T cell immune response, including, for example, cytokine-producing T cells or cytotoxic T cells, to protect said animal from disease, whether that disease is already established within the individual or not. An immunological response in a mammal may also be induced by a method comprises delivering a polypeptide of the present invention via a vector directing expression of the polynucleotide and coding for the polypeptide in vivo in order to induce such an immunological response to produce antibody to protect said animal from diseases of the invention. One way of administering the vector is by accelerating it into the desired cells as a coating on particles or otherwise. Such nucleic acid vector may comprise DNA, RNA, a modified nucleic acid, or a DNA/RNA hybrid. For use a vaccine, a polypeptide or a nucleic acid vector will be normally provided as a vaccine formulation (composition). The formulation may further comprise a suitable carrier. Since a polypeptide may be broken down in the stomach, it is preferably administered parenterally (for instance, subcutaneous, intramuscular, intravenous, or intra-dermal injection). Formulations suitable for parenteral administration include aqueous and non-aqueous sterile injection solutions that may contain anti-oxidants, buffers, bacteriostats and solutes that render the formulation instonic with the blood of the recipient; and aqueous and non-aqueous sterile suspensions that may include suspending agents or thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example, sealed ampoules and vials and may be stored in a freeze-dried condition requiring only the addition of the sterile liquid carrier immediately prior to use. The vaccine formulation may also include adjuvant systems for enhancing the immunogenicity of the formulation, such as oil-in water systems and other systems known in the art. The dosage will depend on the specific activity of the vaccine and can be readily determined by routine experimentation.  
      Polypeptides of the present invention have one or more biological functions that are of relevance in one or more disease states, in particular the diseases of the invention hereinbefore mentioned. It is therefore useful to identify compounds that stimulate or inhibit the function or level of the polypeptide. Accordingly, in a further aspect, the present invention provides for a method of screening compounds to identify those that stimulate or inhibit the function or level of the polypeptide. Such methods identify agonists or antagonists that may be employed for therapeutic and prophylactic purposes for such diseases of the invention as hereinbefore mentioned. Compounds may be identified from a variety of sources, for example, cells, cell-free preparations, chemical libraries, collections of chemical compounds, and natural product mixtures. Such agonists or antagonists so-identified may be natural or modified substrates, ligands, receptors, enzymes, etc., as the case may be, of the polypeptide; a structural or functional mimetic thereof (see Coligan et al., Current Protocols in Immunology 1(2):Chapter 5 (1991)) or a small molecule. Such small molecules preferably have a molecular weight below 2,000 daltons, more preferably between 300 and 1,000 daltons, and most preferably between 400 and 700 daltons. It is preferred that these small molecules are organic molecules.  
      The screening method may simply measure the binding of a candidate compound to the polypeptide, or to cells or membranes bearing the polypeptide, or a fusion protein thereof, by means of a label directly or indirectly associated with the candidate compound. Alternatively, the screening method may involve measuring or detecting (qualitatively or quantitatively) the competitive binding of a candidate compound to the polypeptide against a labeled competitor (e.g agonist or antagonist). Further, these screening methods may test whether the candidate compound results in a signal generated by activation or inhibition of the polypeptide, using detection systems appropriate to the cells bearing the polypeptide. Inhibitors of activation are generally assayed in the presence of a known agonist and the effect on activation by the agonist by the presence of the candidate compound is observed. Further, the screening methods may simply comprise the steps of mixing a candidate compound with a solution containing a polypeptide of the present invention, to form a mixture, measuring an activity of the genes set forth in Table I in the mixture, and comparing activity of the mixture of the genes set forth in Table I to a control mixture which contains no candidate compound.  
      Polypeptides of the present invention may be employed in conventional low capacity screening methods and also in high-throughput screening (HTS) formats. Such HTS formats include not only the well-established use of 96- and, more recently, 384-well micotiter plates but also emerging methods such as the nanowell method described by Schullek et al, Anal Biochem., 246, 20-29, (1997).  
      Fusion proteins, such as those made from Fc portion and polypeptide of the genes set forth in Table I, as hereinbefore described, can also be used for high-throughput screening assays to identify antagonists for the polypeptide of the present invention (see D. Bennett et al., J Mol Recognition, 8:52-58 (1995); and K. Johanson et al., J Biol Chem, 270(16):9459-9471 (1995)).  
      The polynucleotides, polypeptides and antibodies to the polypeptide of the present invention may also be used to configure screening methods for detecting the effect of added compounds on the production of mRNA and polypeptide in cells. For example, an ELISA assay may be constructed for measuring secreted or cell associated levels of polypeptide using monoclonal and polyclonal antibodies by standard methods known in the art. This can be used to discover agents that may inhibit or enhance the production of polypeptide (also called antagonist or agonist, respectively) from suitably manipulated cells or tissues.  
      A polypeptide of the present invention may be used to identify membrane bound or soluble receptors, if any, through standard receptor binding techniques known in the art. These include, but are not limited to, ligand binding and crosslinking assays in which the polypeptide is labeled with a radioactive isotope (for instance,  125 I), chemically modified (for instance, biotinylated), labeled with a reare earth element (for instance, europium), or fused to a peptide sequence suitable for detection or purification, and incubated with a source of the putative receptor (cells, cell membranes, cell supernatants, tissue extracts, bodily fluids). Other methods include biophysical techniques such as surface plasmon resonance and spectroscopy. These screening methods may also be used to identify agonists and antagonists of the polypeptide that compete with the binding of the polypeptide to its receptors, if any. Standard methods for conducting such assays are well understood in the art.  
      Examples of antagonists of polypeptides of the present invention include antibodies or, in some cases, oligonucleotides or proteins that are closely related to the ligands, substrates, receptors, enzymes, etc., as the case may be, of the polypeptide, e.g., a fragment of the ligands, substrates, receptors, enzymes, etc.; or a small molecule that binds to the polypeptide of the present invention but does not elicit a response, so that the activity of the polypeptide is prevented.  
      Screening methods may also involve the use of transgenic technology and the genes set forth in Table I. The art of constructing transgenic animals is well established. For example, the genes set forth in Table I may be introduced through microinjection into the male pronucleus of fertilized oocytes, retroviral transfer into pre- or post-implantation embryos, or injection of genetically modified, such as by electroporation, embryonic stem cells into host blastocysts. Particularly useful transgenic animals are so-called “knock-in” animals in which an animal gene is replaced by the human equivalent within the genome of that animal. Knock-in transgenic animals are useful in the drug discovery process, for target validation, where the compound is specific for the human target. Other useful transgenic animals are so-called “knock-out” animals in which the expression of the animal ortholog of a polypeptide of the present invention and encoded by an endogenous DNA sequence in a cell is partially or completely annulled. The gene knock-out may be targeted to specific cells or tissues, may occur only in certain cells or tissues as a consequence of the limitations of the technology, or may occur in all, or substantially all, cells in the animal. Transgenic animal technology also offers a whole animal expression-cloning system in which introduced genes are expressed to give large amounts of polypeptides of the present invention  
      Screening kits for use in the above described methods form a further aspect of the present invention. Such screening kits comprise: 
          (a) a polypeptide of the present invention;     (b) a recombinant cell expressing a polypeptide of the present invention;     (c) a cell membrane expressing a polypeptide of the present invention; or     (d) an antibody to a polypeptide of the present invention;     which polypeptide is preferably that set forth in the Sequence Listing.        

      It will be appreciated that in any such kit, (a), (b), (c) or (d) may comprise a substantial component.  
      The polypeptides of the present invention can be formulated into pharmaceutical compositions and administered in the same manner as described for other polypeptides. See, e.g., International Patent Application, Publication No. WO90/02762. Generally, these compositions contain a therapeutically effective amount of a polypeptide of this invention and an acceptable pharmaceutical carrier. Suitable carriers are well known to those of skill in the art and include, for example, saline. Alternatively, such compositions may include conventional delivery systems into which polypeptide of the invention is incorporated. Optionally, these compositions may contain other active ingredients.  
      The polypeptides of this invention may be administered by any appropriate internal route, and may be repeated as needed, e.g., as frequently as one to three times daily for between 1 day to about three weeks to once per week or once biweekly. Alternatively, the peptide maybe altered to reduce charge density and thus allow oral bioavailability. The dose and duration of treatment relates to the relative duration of the molecules of the present invention in the human circulation, and can be adjusted by one of skill in the art depending upon the condition being treated and the general health of the patient.  
      As used herein, the term “pharmaceutical” includes veterinary applications of the invention. The term “therapeutically effective amount” refers to that amount of therapeutic agent, which is useful for alleviating a selected condition.  
      In a specific embodiment, nucleic acids comprising sequences encoding the instant polypeptides or functional derivatives thereof, are administered to treat, inhibit or prevent a disease or disorder associated with aberrant expression and/or activity of a polypeptide of the invention, by way of gene therapy. “Gene therapy” refers to therapy performed by the administration to a subject of an expressed or expressible nucleic acid. In this embodiment of the invention, the nucleic acids produce their encoded protein that mediates a therapeutic effect.  
      Any of the methods for gene therapy available in the art can be used according to the present invention. Exemplary methods are described below.  
      For general reviews of the methods of gene therapy, see Goldspiel et al., Clinical Pharmacy 12:488-505 (1993); Wu and Wu, Biotherapy 3:87-95 (1991); Tolstoshev, Ann. Rev. Pharmacol. Toxicol. 32:573-596 (1993); Mulligan, Science 260:926-932 (1993); and Morgan and Anderson, Ann. Rev. Biochem. 62:191-217 (1993); May, TIBTECH 11(5):155-215 (1993). Methods commonly known in the art of recombinant DNA technology which can be used are described in Ausubel et al. (eds.), Current Protocols in Molecular Biology, John Wiley &amp; Sons, New York (1993); and Kriegler, Gene Transfer and Expression, A Laboratory Manual, Stockton Press, New York (1990).  
      In a preferred aspect, the compound comprises nucleic acid sequences encoding a polypeptide, said nucleic acid sequences being part of expression vectors that express a polypeptide in a suitable host. In particular, such nucleic acid sequences have promoters operably linked to the polypeptide coding region, said promoter being inducible or constitutive, and, optionally, tissue-specific. In another particular embodiment, nucleic acid molecules are used in which the polypeptide coding sequences and any other desired sequences are flanked by regions that promote homologous recombination at a desired site in the genome, thus providing for intrachromosomal expression of the polypeptide-encoding nucleic acids (Koller and Smithies, Proc. Natl. Acad. Sci. USA 86:8932-8935 (1989); Zijlstra et al., Nature 342:435-438 (1989).  
      Delivery of the nucleic acids into a patient may be either direct, in which case the patient is directly exposed to the nucleic acid or nucleic acid-carrying vectors, or indirect, in which case, cells are first transformed with the nucleic acids in vitro, then transplanted into the patient. These two approaches are known, respectively, as in vivo or ex vivo gene therapy.  
      In a specific embodiment, the nucleic acid sequences are directly administered in vivo, where it is expressed to produce the encoded product. This can be accomplished by any of numerous methods known in the art, e.g., by constructing them as part of an appropriate nucleic acid expression vector and administering it so that they become intracellular, e.g., by infection using defective or attenuated retrovirals or other viral vectors (see U.S. Pat. No. 4,980,286), or by direct injection of naked DNA, or by use of microparticle bombardment (e.g., a gene gun; Biolistic, Dupont), or coating with lipids or cell-surface receptors or transfecting agents, encapsulation in liposomes, microparticles, or microcapsules, or by administering them in linkage to a peptide which is known to enter the nucleus, by administering it in linkage to a ligand subject to receptor-mediated endocytosis (see, e.g., Wu and Wu, J. Biol. Chem. 262:4429-4432 (1987)) (which can be used to target cell types specifically expressing the receptors), etc. In another embodiment, nucleic acid-ligand complexes can be formed in which the ligand comprises a fusogenic viral peptide to disrupt endosomes, allowing the nucleic acid to avoid lysosomal degradation. In yet another embodiment, the nucleic acid can be targeted in vivo for cell specific uptake and expression, by targeting a specific receptor (see, e.g., PCT Publications WO 92/06180; WO 92/22635; WO92/20316; WO93/14188, WO 93/20221). Alternatively, the nucleic acid can be introduced intracellularly and incorporated within host cell DNA for expression, by homologous recombination (Koller and Smithies, Proc. Natl. Acad. Sci. USA 86:8932-8935 (1989); Zijlstra et al., Nature 342:435-438 (1989)).  
      In a specific embodiment, viral vectors that contain nucleic acid sequences encoding a polypeptide of the invention are used. For example, a retroviral vector can be used (see Miller et al., Meth. Enzymol. 217:581-599 (1993)). These retroviral vectors contain the components necessary for the correct packaging of the viral genome and integration into the host cell DNA. The nucleic acid sequences encoding the antibody to be used in gene therapy are cloned into one or more vectors, which facilitates delivery of the gene into a patient. More detail about retroviral vectors can be found in Boesen et al., Biotherapy 6:291-302 (1994), which describes the use of a retroviral vector to deliver the mdr1 gene to hematopoietic stem cells in order to make the stem cells more resistant to chemotherapy. Other references illustrating the use of retroviral vectors in gene therapy are: Clowes et al., J. Clin. Invest. 93:644-651 (1994); Kiem et al., Blood 83:1467-1473 (1994); Salmons and Gunzberg, Human Gene Therapy 4:129-141 (1993); and Grossman and Wilson, Curr. Opin. in Genetics and Devel. 3:110-114 (1993).  
      Adenoviruses are other viral vectors that can be used in gene therapy. Adenoviruses are especially attractive vehicles for delivering genes to respiratory epithelia. Adenoviruses naturally infect respiratory epithelia where they cause a mild disease. Other targets for adenovirus-based delivery systems are liver, the central nervous system, endothelial cells, and muscle. Adenoviruses have the advantage of being capable of infecting non-dividing cells. Kozarsky and Wilson, Current Opinion in Genetics and Development 3:499-503 (1993) present a review of adenovirus-based gene therapy. Bout et al., Human Gene Therapy 5:3-10 (1994) demonstrated the use of adenovirus vectors to transfer genes to the respiratory epithelia of rhesus monkeys. Other instances of the use of adenoviruses in gene therapy can be found in Rosenfeld et al., Science 252:431-434 (1991); Rosenfeld et al., Cell 68:143-155 (1992); Mastrangeli et al., J. Clin. Invest. 91:225-234 (1993); PCT Publication WO94/12649; and Wang, et al., Gene Therapy 2:775-783 (1995). In a preferred embodiment, adenovirus vectors are used.  
      Adeno-associated virus (AAV) has also been proposed for use in gene therapy (Walsh et al., Proc. Soc. Exp. Biol. Med. 204:289-300 (1993); U.S. Pat. No. 5,436,146).  
      Another approach to gene therapy involves transferring a gene to cells in tissue culture by such methods as electroporation, lipofection, calcium phosphate mediated transfection, or viral infection. Usually, the method of transfer includes the transfer of a selectable marker to the cells. The cells are then placed under selection to isolate those cells that have taken up and are expressing the transferred gene. Those cells are then delivered to a patient.  
      In this embodiment, the nucleic acid is introduced into a cell prior to administration in vivo of the resulting recombinant cell. Such introduction can be carried out by any method known in the art, including but not limited to transfection, electroporation, microinjection, infection with a viral or bacteriophage vector containing the nucleic acid sequences, cell fusion, chromosome-mediated gene transfer, microcell-mediated gene transfer, spheroplast fusion, etc. Numerous techniques are known in the art for the introduction of foreign genes into cells (see, e.g., Loeffler and Behr, Meth. Enzymol. 217:599-618 (1993); Cohen et al., Meth. Enzymol. 217:618-644 (1993); Cline, Pharmac. Ther. 29:69-92m (1985) and may be used in accordance with the present invention, provided that the necessary developmental and physiological functions of the recipient cells are not disrupted. The technique should provide for the stable transfer of the nucleic acid to the cell, so that the nucleic acid is expressible by the cell and preferably heritable and expressible by its cell progeny.  
      The resulting recombinant cells can be delivered to a patient by various methods known in the art. Recombinant blood cells (e.g., hematopoietic stem or progenitor cells) are preferably administered intravenously. The amount of cells envisioned for use depends on the desired effect, patient state, etc., and can be determined by one skilled in the art. Cells into which a nucleic acid can be introduced for purposes of gene therapy encompass any desired, available cell type, and include but are not limited to epithelial cells, endothelial cells, keratinocytes, fibroblasts, muscle cells, hepatocytes; blood cells such as Tlymphocytes, Blymphocytes, monocytes, macrophages, neutrophils, eosinophils, megakaryocytes, granulocytes; various stem or progenitor cells, in particular hematopoietic stem or progenitor cells, e.g., as obtained from bone marrow, umbilical cord blood, peripheral blood, fetal liver, etc.  
      In a preferred embodiment, the cell used for gene therapy is autologous to the patient.  
      In an embodiment in which recombinant cells are used in gene therapy, nucleic acid sequences encoding a polypeptide are introduced into the cells such that they are expressible by the cells or their progeny, and the recombinant cells are then administered in vivo for therapeutic effect. In a specific embodiment, stem or progenitor cells are used. Any stem and/or progenitor cells which can be isolated and maintained in vitro can potentially be used in accordance with this embodiment of the present invention (see e.g. PCT Publication WO 94/08598; Stemple and Anderson, Cell 71:973-985 (1992); Rheinwald, Meth. Cell Bio. 21A:229 (1980); and Pittelkow and Scott, Mayo Clinic Proc. 61:771 (1986)).  
      In a specific embodiment, the nucleic acid to be introduced for purposes of gene therapy comprises an inducible promoter operably linked to the coding region, such that expression of the nucleic acid is controllable by controlling the presence or absence of the appropriate inducer of transcription.  
      The invention provides methods of treatment, inhibition and prophylaxis by administration to a subject of an effective amount of a compound or pharmaceutical composition of the invention, preferably a polypeptide of the invention. In a preferred aspect, the compound is substantially purified (e.g., substantially free from substances that limit its effect or produce undesired side-effects). The subject is preferably an animal, including but not limited to animals such as cows, pigs, horses, chickens, cats, dogs, etc., and is preferably a mammal, and most preferably human.  
      Formulations and methods of administration that can be employed when the compound comprises a nucleic acid or a polypeptide are described above; additional appropriate formulations and routes of administration can be selected from among those described herein below.  
      Various delivery systems are known and can be used to administer a compound of the invention, e.g., encapsulation in liposomes, microparticles, microcapsules, recombinant cells capable of expressing the compound, receptor-mediated endocytosis (see, e.g., Wu and Wu, J. Biol. Chem. 262:4429-4432 (1987)), construction of a nucleic acid as part of a retroviral or other vector, etc. Methods of introduction include but are not limited to intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, intranasal, epidural, and oral routes. The compounds or compositions may be administered by any convenient route, for example by infusion or bolus injection, by absorption through epithelial or mucocutaneous linings (e.g., oral mucosa, rectal and intestinal mucosa, etc.) and may be administered together with other biologically active agents. Administration can be systemic or local. In addition, it may be desirable to introduce the pharmaceutical compounds or compositions of the invention into the central nervous system by any suitable route, including intraventricular and intrathecal injection; intraventricular injection may be facilitated by an intraventricular catheter, for example, attached to a reservoir, such as an Ommaya reservoir. Pulmonary administration can also be employed, e.g., by use of an inhaler or nebulizer, and formulation with an aerosolizing agent.  
      In a specific embodiment, it may be desirable to administer the pharmaceutical compounds or compositions of the invention locally to the area in need of treatment; this may be achieved by, for example, and not by way of limitation, local infusion during surgery, topical application, e.g., in conjunction with a wound dressing after surgery, by injection, by means of a catheter, by means of a suppository, or by means of an implant, said implant being of a porous, non-porous, or gelatinous material, including membranes, such as sialastic membranes, or fibers. Preferably, when administering a protein, including an antibody, of the invention, care must be taken to use materials to which the protein does not absorb.  
      In another embodiment, the compound or composition can be delivered in a vesicle, in particular a liposome (see Langer, Science 249:1527-1533 (1990); Treat et al., in Liposomes in the Therapy of Infectious Disease and Cancer, Lopez-Berestein and Fidler (eds.), Liss, N.Y., pp. 353-365 (1989); Lopez-Berestein, ibid., pp. 317-327; see generally ibid.)  
      In yet another embodiment, the compound or composition can be delivered in a controlled release system. In one embodiment, a pump may be used (see Langer, supra; Sefton, CRC Crit. Ref. Biomed. Eng. 14:201 (1987); Buchwald et al., Surgery 88:507 (1980); Saudek et al., N. Engl. J. Med. 321:574 (1989)). In another embodiment, polymeric materials can be used (see Medical Applications of Controlled Release, Langer and Wise (eds.), CRC Pres., Boca Raton, Fla. (1974); Controlled Drug Bioavailability, Drug Product Design and Performance, Smolen and Ball (eds.), Wiley, N.Y. (1984); Ranger and Peppas, J., Macromol. Sci. Rev. Macromol. Chem. 23:61 (1983); see also Levy et al., Science 228:190 (1985); During et al., Ann. Neurol. 25:351 (1989); Howard et al., J.Neurosurg. 71:105 (1989)). In yet another embodiment, a controlled release system can be placed in proximity of the therapeutic target, i.e., the brain, thus requiring only a fraction of the systemic dose (see, e.g., Goodson, in Medical Applications of Controlled Release, supra, vol. 2, pp. 115-138 (1984)).  
      Other controlled release systems are discussed in the review by Langer (Science 249:1527-1533 (1990)).  
      In a specific embodiment where the compound of the invention is a nucleic acid encoding a polypeptide, the nucleic acid can be administered in vivo to promote expression of its encoded protein, by constructing it as part of an appropriate nucleic acid expression vector and administering it so that it becomes intracellular, e.g., by use of a retroviral vector (see U.S. Pat. No. 4,980,286), or by direct injection, or by use of microparticle bombardment (e.g., a gene gun; Biolistic, Dupont), or coating with lipids or cell-surface receptors or transfecting agents, or by administering it in linkage to a homeobox- like peptide which is known to enter the nucleus (see e.g., Joliot et al., Proc. Natl. Acad. Sci. USA 88:1864-1868 (1991)), etc. Alternatively, a nucleic acid can be introduced intracellularly and incorporated within host cell DNA for expression, by homologous recombination.  
      The present invention also provides pharmaceutical compositions. Such compositions comprise a therapeutically effective amount of a compound, and a pharmaceutically acceptable carrier. In a specific embodiment, the term “pharmaceutically acceptable” means approved by a regulatory agency of the Federal or a state government or listed in the U.S. Pharmacopeia or other generally recognized pharmacopeia for use in animals, and more particularly in humans. The term “carrier” refers to a diluent, adjuvant, excipient, or vehicle with which the therapeutic is administered. Such pharmaceutical carriers can be sterile liquids, such as water and oils, including those of petroleum, animal, vegetable or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like. Water is a preferred carrier when the pharmaceutical composition is administered intravenously. Saline solutions and aqueous dextrose and glycerol solutions can also be employed as liquid carriers, particularly for injectable solutions. Suitable pharmaceutical excipients include starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, dried skim milk, glycerol, propylene, glycol, water, ethanol and the like. The composition, if desired, can also contain minor amounts of wetting or emulsifying agents, or pH buffering agents. These compositions can take the form of solutions, suspensions, emulsion, tablets, pills, capsules, powders, sustained-release formulations and the like. The composition can be formulated as a suppository, with traditional binders and carriers such as triglycerides. Oral formulation can include standard carriers such as pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, magnesium carbonate, etc. Examples of suitable pharmaceutical carriers are described in “Remington&#39;s Pharmaceutical Sciences” by E. W. Martin. Such compositions will contain a therapeutically effective amount of the compound, preferably in purified form, together with a suitable amount of carrier so as to provide the form for proper administration to the patient. The formulation should suit the mode of administration.  
      In a preferred embodiment, the composition is formulated in accordance with routine procedures as a pharmaceutical composition adapted for intravenous administration to human beings. Typically, compositions for intravenous administration are solutions in sterile isotonic aqueous buffer. Where necessary, the composition may also include a solubilizing agent and a local anesthetic such as lignocaine to ease pain at the site of the injection. Generally, the ingredients are supplied either separately or mixed together in unit dosage form, for example, as a dry lyophilized powder or water free concentrate in a hermetically sealed container such as an ampoule or sachette indicating the quantity of active agent. Where the composition is to be administered by infusion, it can be dispensed with an infusion bottle containing sterile pharmaceutical grade water or saline. Where the composition is administered by injection, an ampoule of sterile water for injection or saline can be provided so that the ingredients may be mixed prior to administration.  
      The compounds of the invention can be formulated as neutral or salt forms. Pharmaceutically acceptable salts include those formed with anions such as those derived from hydrochloric, phosphoric, acetic, oxalic, tartaric acids, etc., and those formed with cations such as those derived from sodium, potassium, ammonium, calcium, ferric hydroxides, isopropylamine, triethylamine, 2-ethylamino ethanol, histidine, procaine, etc.  
      The amount of the compound of the invention which will be effective in the treatment, inhibition and prevention of a disease or disorder associated with aberrant expression and/or activity of a polypeptide of the invention can be determined by standard clinical techniques. In addition, in vitro assays may optionally be employed to help identify optimal dosage ranges. The precise dose to be employed in the formulation will also depend on the route of administration, and the seriousness of the disease or disorder, and should be decided according to the judgment of the practitioner and each patient&#39;s circumstances. Effective doses may be extrapolated from dose-response curves derived from in vitro or animal model test systems.  
      For polypeptides, the dosage administered to a patient is typically 0.1 mg/kg to 100 mg/kg of the patient&#39;s body weight. Preferably, the dosage administered to a patient is between 0.1 mg/kg and 20 mg/kg of the patient&#39;s body weight, more preferably 1 mg/kg to 10 mg/kg of the patient&#39;s body weight. Generally, human polypeptides have a longer half-life within the human body than polypeptides from other species due to the immune response to the foreign polypeptides. Thus, lower dosages of human polypeptides and less frequent administration is often possible. Further, the dosage and frequency of administration of polypeptides of the invention may be reduced by enhancing uptake and tissue penetration (e.g., into the brain) of the polypeptides by modifications such as, for example, lipidation.  
      The invention also provides a pharmaceutical pack or kit comprising one or more containers filled with one or more of the ingredients of the pharmaceutical compositions of the invention. Optionally associated with such container(s) can be a notice in the form prescribed by a governmental agency regulating the manufacture, use or sale of pharmaceuticals or biological products, which notice reflects approval by the agency of manufacture, use or sale for human administration.  
      This invention provides for a pharmaceutical composition which comprises a polypeptide of this invention and a pharmaceutically acceptable carrier, diluent or excipient. Accordingly, the polypeptide may be used in the manufacture of a medicament. Pharmaceutical compositions of the invention may be formulated as solutions or lyophilized powders for parenteral administration. Powders may be reconstituted by addition of a suitable diluent or other pharmaceutically acceptable carrier prior to use. The liquid formulation may be a buffered, isotonic, aqueous solution. Examples of suitable diluents are normal isotonic saline solution, standard 5% dextrose in water or buffered sodium or ammonium acetate solution. Such formulation is especially suitable for parenteral administration, but may also be used for oral administration or contained in a metered dose inhaler or nebulizer for insufflation. It may be desirable to add excipients such as polyvinylpyrrolidone, gelatin, hydroxy cellulose, acacia, polyethylene glycol, mannitol, sodium chloride or sodium citrate.  
      Alternately, the polypeptide may be encapsulated, tableted or prepared in an emulsion or syrup for oral administration. Pharmaceutically acceptable solid or liquid carriers may be added to enhance or stabilize the composition, or to facilitate preparation of the composition. Solid carriers include starch, lactose, calcium sulfate dihydrate, terra alba, magnesium stearate or stearic acid, talc, pectin, acacia, agar or gelatin. Liquid carriers include syrup, peanut oil, olive oil, saline and water. The carrier may also include a sustained release material such as glyceryl monostearate or glyceryl distearate, alone or with a wax. The amount of solid carrier varies but, preferably, will be between about 20 mg to about 1 g per dosage unit. The pharmaceutical preparations are made following the conventional techniques of pharmacy involving milling, mixing, granulating, and compressing, when necessary, for tablet forms; or milling, mixing and filling for hard gelatin capsule forms. When a liquid carrier is used, the preparation will be in the form of a syrup, elixir, emulsion or an aqueous or non-aqueous suspension. Such a liquid formulation may be administered directly p.o. or filled into a soft gelatin capsule.  
      The mode of administration of a polypeptide of the invention may be any suitable route which delivers the agent to the host. The polypeptides and pharmaceutical compositions of the invention are particularly useful for parenteral administration, i.e., subcutaneously, intramuscularly, intravenously or intranasally.  
      Polypeptide of the invention may be prepared as pharmaceutical compositions containing an effective amount of a polypeptide of the invention as an active ingredient in a pharmaceutically acceptable carrier. In the compositions of the invention, an aqueous suspension or solution containing the polypeptide, preferably buffered at physiological pH, in a form ready for injection is preferred. The compositions for parenteral administration will commonly comprise a solution of the polypeptide of the invention or a cocktail thereof dissolved in an pharmaceutically acceptable carrier, preferably an aqueous carrier. A variety of aqueous carriers may be employed, e.g., 0.4% saline, 0.3% glycine and the like. These solutions are sterile and generally free of particulate matter. These solutions may be sterilized by conventional, well known sterilization techniques (e.g., filtration). The compositions may contain pharmaceutically acceptable auxiliary substances as required to approximate physiological conditions such as pH adjusting and buffering agents, etc. The concentration of the polypeptide of the invention in such pharmaceutical formulation can vary widely, i.e., from less than about 0.5%, usually at or at least about 1% to as much as 15 or 20% by weight and will be selected primarily based on fluid volumes, viscosities, etc., according to the particular mode of administration selected.  
      Thus, a pharmaceutical composition of the invention for intramuscular injection could be prepared to contain 1 mL sterile buffered water, and between about 1 ng to about 100 mg, e.g. about 50 ng to about 30 mg or more preferably, about 5 mg to about 25 mg, of a polypeptide of the invention. Similarly, a pharmaceutical composition of the invention for intravenous infusion could be made up to contain about 250 mL of sterile Ringer&#39;s solution, and about 1 mg to about 30 mg and preferably 5 mg to about 25 mg of a polypeptide of the invention. Actual methods for preparing parenterally administrable compositions are well known or will be apparent to those skilled in the art and are described in more detail in, for example, “Remington&#39;s Pharmaceutical Science”, 15th ed., Mack Publishing Company, Easton, Pa.  
      It is preferred that the polypeptide of the invention, when in a pharmaceutical preparation, be present in unit dose forms. The appropriate therapeutically effective dose can be determined readily by those of skill in the art. Such dose may, if necessary, be repeated at appropriate time intervals selected as appropriate by a physician during the response period.  
      The present invention may be embodied in other specific forms, without departing from the spirit or essential attributes thereof, and, accordingly, reference should be made to the appended claims, rather than to the foregoing specification or following examples, as indicating the scope of the invention.  
      Glossary  
      The following definitions are provided to facilitate understanding of certain terms used frequently hereinbefore.  
      “Antibodies” as used herein includes polyclonal and monoclonal antibodies, chimeric, single chain, and humanized antibodies and fully human antibodies produced in transgeneic mice, where the mouse antibody coding genes have been suppressed or replaced and substituted with human antibody coding genes, as well as Fab fragments, including the products of an Fab or other immunoglobulin expression library.  
      “Isolated” means altered “by the hand of man” from its natural state, i.e., if it occurs in nature, it has been changed or removed from its original environment, or both. For example, a polynucleotide or a polypeptide naturally present in a living organism is not “isolated,” but the same polynucleotide or polypeptide separated from the coexisting materials of its natural state is “isolated”, as the term is employed herein. Moreover, a polynucleotide or polypeptide that is introduced into an organism by transformation, genetic manipulation or by any other recombinant method is “isolated” even if it is still present in said organism, which organism may be living or non-living.  
      “Secreted protein activity or secreted polypeptide activity” or “biological activity of the secreted protein or secreted polypeptide” refers to the metabolic or physiologic function of said secreted protein including similar activities or improved activities or these activities with decreased undesirable side-effects. Also included are antigenic and immunogenic activities of said secreted protein.  
      “Secreted protein gene” refers to a polynucleotide comprising any of the attached nucleotide sequences or allelic variants thereof and/or their complements.  
      “Polynucleotide” generally refers to any polyribonucleotide (RNA) or polydeoxribonucleotide (DNA), which may be unmodified or modified RNA or DNA. “Polynucleotides” include, without limitation, single- and double-stranded DNA, DNA that is a mixture of single- and double-stranded regions, single- and double-stranded RNA, and RNA that is mixture of single- and double-stranded regions, hybrid molecules comprising DNA and RNA that may be single-stranded or, more typically, double-stranded or a mixture of single- and double-stranded regions. In addition, “polynucleotide” refers to triple-stranded regions comprising RNA or DNA or both RNA and DNA. The term “polynucleotide” also includes DNAs or RNAs containing one or more modified bases and DNAs or RNAs with backbones modified for stability or for other reasons. “Modified” bases include, for example, tritylated bases and unusual bases such as inosine. A variety of modifications may be made to DNA and RNA; thus, “polynucleotide” embraces chemically, enzymatically or metabolically modified forms of polynucleotides as typically found in nature, as well as the chemical forms of DNA and RNA characteristic of viruses and cells. “Polynucleotide” also embraces relatively short polynucleotides, often referred to as oligonucleotides.  
      “Polypeptide” refers to any polypeptide comprising two or more amino acids joined to each other by peptide bonds or modified peptide bonds, i.e., peptide isosteres. “Polypeptide” refers to both short chains, commonly referred to as peptides, oligopeptides or oligomers, and to longer chains, generally referred to as proteins. Polypeptides may contain amino acids other than the 20 gene-encoded amino acids. “Polypeptides” include amino acid sequences modified either by natural processes, such as post-translational processing, or by chemical modification techniques that are well known in the art. Such modifications are well described in basic texts and in more detailed monographs, as well as in a voluminous research literature. Modifications may occur anywhere in a polypeptide, including the peptide backbone, the amino acid side-chains and the amino or carboxyl termini. It will be appreciated that the same type of modification may be present to the same or varying degrees at several sites in a given polypeptide. Also, a given polypeptide may contain many types of modifications. Polypeptides may be branched as a result of ubiquitination, and they may be cyclic, with or without branching. Cyclic, branched and branched cyclic polypeptides may result from post-translation natural processes or may be made by synthetic methods. Modifications include acetylation, acylation, ADP-ribosylation, amidation, biotinylation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent cross-links, formation of cystine, formation of pyroglutamate, formylation, gamma-carboxylation, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolytic processing, phosphorylation, prenylation, racemization, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to proteins such as arginylation, and ubiquitination (see, for instance, Proteins—Structure and Molecular Properties, 2nd Ed., T. E. Creighton, W. H. Freeman and Company, New York, 1993; Wold, F., Post-translational Protein Modifications: Perspectives and Prospects, 1-12, in Post-translational Covalent Modification of Proteins, B. C. Johnson, Ed., Academic Press, New York, 1983; Seifter et al., “Analysis for protein modifications and nonprotein cofactors”, Meth Enzymol, 182, 626-646, 1990, and Rattan et al., “Protein Synthesis: Post-translational Modifications and Aging”, Ann NY Acad Sci, 663, 48-62, 1992).  
      “Fragment” of a polypeptide sequence refers to a polypeptide sequence that is shorter than the reference sequence but that retains essentially the same biological function or activity as the reference polypeptide. “Fragment” of a polynucleotide sequence refers to a polynucleotide sequence that is shorter than the reference sequence set forth in the Sequence Listing.  
      “Variant” refers to a polynucleotide or polypeptide that differs from a reference polynucleotide or polypeptide, but retains the essential properties thereof. A typical variant of a polynucleotide differs in nucleotide sequence from the reference polynucleotide. Changes in the nucleotide sequence of the variant may or may not alter the amino acid sequence of a polypeptide encoded by the reference polynucleotide. Nucleotide changes may result in amino acid substitutions, additions, deletions, fusions and truncations in the polypeptide encoded by the reference sequence, as discussed below. A typical variant of a polypeptide differs in amino acid sequence from the reference polypeptide. Generally, alterations are limited so that the sequences of the reference polypeptide and the variant are closely similar overall and, in many regions, identical. A variant and reference polypeptide may differ in amino acid sequence by one or more substitutions, insertions, deletions in any combination. A substituted or inserted amino acid residue may or may not be one encoded by the genetic code. Typical conservative substitutions include Gly, Ala; Val, Ile, Leu; Asp, Glu; Asn, Gin; Ser, Thr; Lys, Arg; and Phe and Tyr. A variant of a polynucleotide or polypeptide may be naturally occurring such as an allele, or it may be a variant that is not known to occur naturally. Non-naturally occurring variants of polynucleotides and polypeptides may be made by mutagenesis techniques or by direct synthesis. Also included as variants are polypeptides having one or more post-translational modifications, for instance glycosylation, phosphorylation, methylation, ADP ribosylation and the like. Embodiments include methylation of the N-terminal amino acid, phosphorylations of serines and threonines and modification of C-terminal glycines.  
      “Allele” refers to one of two or more alternative forms of a gene occurring at a given locus in the genome.  
      “Polymorphism” refers to a variation in nucleotide sequence (and encoded polypeptide sequence, if relevant) at a given position in the genome within a population.  
      “Single Nucleotide Polymorphism” (SNP) refers to the occurrence of nucleotide variability at a single nucleotide position in the genome, within a population. An SNP may occur within a gene or within intergenic regions of the genome. SNPs can be assayed using Allele Specific Amplification (ASA). For the process at least 3 primers are required. A common primer is used in reverse complement to the polymorphism being assayed. This common primer can be between 50 and 1500 bps from the polymorphic base. The other two (or more) primers are identical to each other except that the final 3′ base wobbles to match one of the two (or more) alleles that make up the polymorphism. Two (or more) PCR reactions are then conducted on sample DNA, each using the common primer and one of the Allele Specific Primers.  
      “Splice Variant” as used herein refers to cDNA molecules produced from RNA molecules initially transcribed from the same genomic DNA sequence but which have undergone alternative RNA splicing. Alternative RNA splicing occurs when a primary RNA transcript undergoes splicing, generally for the removal of introns, which results in the production of more than one mRNA molecule each of that may encode different amino acid sequences. The term splice variant also refers to the proteins encoded by the above cDNA molecules.  
      “Identity” reflects a relationship between two or more polypeptide sequences or two or more polynucleotide sequences, determined by comparing the sequences. In general, identity refers to an exact nucleotide to nucleotide or amino acid to amino acid correspondence of the two polynucleotide or two polypeptide sequences, respectively, over the length of the sequences being compared.  
      “% Identity”—For sequences where there is not an exact correspondence, a “% identity” may be determined. In general, the two sequences to be compared are aligned to give a maximum correlation between the sequences. This may include inserting “gaps” in either one or both sequences, to enhance the degree of alignment. A % identity may be determined over the whole length of each of the sequences being compared (so-called global alignment), that is particularly suitable for sequences of the same or very similar length, or over shorter, defined lengths (so-called local alignment), that is more suitable for sequences of unequal length.  
      “Similarity” is a further, more sophisticated measure of the relationship between two polypeptide sequences. In general, “similarity” means a comparison between the amino acids of two polypeptide chains, on a residue by residue basis, taking into account not only exact correspondences between a between pairs of residues, one from each of the sequences being compared (as for identity) but also, where there is not an exact correspondence, whether, on an evolutionary basis, one residue is a likely substitute for the other. This likelihood has an associated “score” from which the “% similarity” of the two sequences can then be determined.  
      Methods for comparing the identity and similarity of two or more sequences are well known in the art. Thus for instance, programs available in the Wisconsin Sequence Analysis Package, version 9.1 (Devereux J et al, Nucleic Acids Res, 12, 387-395, 1984, available from Genetics Computer Group, Madison, Wis., USA), for example the programs BESTFIT and GAP, may be used to determine the % identity between two polynucleotides and the % identity and the % similarity between two polypeptide sequences. BESTFIT uses the “local homology” algorithm of Smith and Waterman (J Mol Biol, 147,195-197, 1981, Advances in Applied Mathematics, 2, 482-489, 1981) and finds the best single region of similarity between two sequences. BESTFIT is more suited to comparing two polynucleotide or two polypeptide sequences that are dissimilar in length, the program assuming that the shorter sequence represents a portion of the longer. In comparison, GAP aligns two sequences, finding a “maximum similarity”, according to the algorithm of Neddleman and Wunsch (J Mol Biol, 48, 443-453, 1970). GAP is more suited to comparing sequences that are approximately the same length and an alignment is expected over the entire length. Preferably, the parameters “Gap Weight” and “Length Weight” used in each program are 50 and 3, for polynucleotide sequences and 12 and 4 for polypeptide sequences, respectively. Preferably, % identities and similarities are determined when the two sequences being compared are optimally aligned.  
      Other programs for determining identity and/or similarity between sequences are also known in the art, for instance the BLAST family of programs (Altschul S F et al, J Mol Biol, 215, 403-410, 1990, Altschul S F et al, Nucleic Acids Res., 25:389-3402, 1997, available from the National Center for Biotechnology Information (NCBI), Bethesda, Md., USA and accessible through the home page of the NCBI at www.ncbi.nlm.nih.gov) and FASTA (Pearson W R, Methods in Enzymology, 183, 63-99, 1990; Pearson W R and Lipman D J, Proc Nat Acad Sci USA, 85, 2444-2448,1988, available as part of the Wisconsin Sequence Analysis Package).  
      Preferably, the BLOSUM62 amino acid substitution matrix (Henikoff S and Henikoff J G, Proc. Nat. Acad Sci. USA, 89, 10915-10919, 1992) is used in polypeptide sequence comparisons including where nucleotide sequences are first translated into amino acid sequences before comparison.  
      Preferably, the program BESTFIT is used to determine the % identity of a query polynucleotide or a polypeptide sequence with respect to a reference polynucleotide or a polypeptide sequence, the query and the reference sequence being optimally aligned and the parameters of the program set at the default value, as hereinbefore described.  
      “Identity Index” is a measure of sequence relatedness which may be used to compare a candidate sequence (polynucleotide or polypeptide) and a reference sequence. Thus, for instance, a candidate polynucleotide sequence having, for example, an Identity Index of 0.95 compared to a reference polynucleotide sequence is identical to the reference sequence except that the candidate polynucleotide sequence may include on average up to five differences per each 100 nucleotides of the reference sequence. Such differences are selected from the group consisting of at least one nucleotide deletion, substitution, including transition and transversion, or insertion. These differences may occur at the 5′ or 3′ terminal positions of the reference polynucleotide sequence or anywhere between these terminal positions, interspersed either individually among the nucleotides in the reference sequence or in one or more contiguous groups within the reference sequence. In other words, to obtain a polynucleotide sequence having an Identity Index of 0.95 compared to a reference polynucleotide sequence, an average of up to 5 in every 100 of the nucleotides of the in the reference sequence may be deleted, substituted or inserted, or any combination thereof, as hereinbefore described. The same applies  mutatis mutandis  for other values of the Identity Index, for instance 0.96, 0.97, 0.98 and 0.99.  
      Similarly, for a polypeptide, a candidate polypeptide sequence having, for example, an Identity Index of 0.95 compared to a reference polypeptide sequence is identical to the reference sequence except that the polypeptide sequence may include an average of up to five differences per each 100 amino acids of the reference sequence. Such differences are selected from the group consisting of at least one amino acid deletion, substitution, including conservative and non-conservative substitution, or insertion. These differences may occur at the amino- or carboxy-terminal positions of the reference polypeptide sequence or anywhere between these terminal positions, interspersed either individually among the amino acids in the reference sequence or in one or more contiguous groups within the reference sequence. In other words, to obtain a polypeptide sequence having an Identity Index of 0.95 compared to a reference polypeptide sequence, an average of up to 5 in every 100 of the amino acids in the reference sequence may be deleted, substituted or inserted, or any combination thereof, as hereinbefore described. The same applies  mutatis mutandis  for other values of the Identity Index, for instance 0.96, 0.97, 0.98 and 0.99.  
      The relationship between the number of nucleotide or amino acid differences and the Identity Index may be expressed in the following equation: 
 
 n   a   ≦x   a −( x   a   ·I),  
 
 in which: 
          n a  is the number of nucleotide or amino acid differences,     x a  is the total number of nucleotides or amino acids in a sequence set forth in the Sequence Listing,     I is the Identity Index,     · is the symbol for the multiplication operator, and 
 
 in which any non-integer product of x a  and I is rounded down to the nearest integer prior to subtracting it from x a . 
       

      “Homolog” is a generic term used in the art to indicate a polynucleotide or polypeptide sequence possessing a high degree of sequence relatedness to a reference sequence. Such relatedness may be quantified by determining the degree of identity and/or similarity between the two sequences as hereinbefore defined. Falling within this generic term are the terms “ortholog”, and “paralog”. “Ortholog” refers to a polynucleotide or polypeptide that is the functional equivalent of the polynucleotide or polypeptide in another species. “Paralog” refers to a polynucleotideor polypeptide that within the same species which is functionally similar.  
      “Fusion protein” refers to a protein encoded by two, often unrelated, fused genes or fragments thereof. In one example, EP-A-0 464 533-A discloses fusion proteins comprising various portions of constant region of immunoglobulin molecules together with another human protein or part thereof. In many cases, employing an immunoglobulin Fc region as a part of a fusion protein is advantageous for use in therapy and diagnosis resulting in, for example, improved pharmacokinetic properties [see, e.g., EP-A 0232 262]. On the other hand, for some uses it would be desirable to be able to delete the Fc part after the fusion protein has been expressed, detected and purified.  
      All publications and references, including but not limited to patents and patent applications, cited in this specification are herein incorporated by reference in their entirety as if each individual publication or reference were specifically and individually indicated to be incorporated by reference herein as being fully set forth. Any patent application to which this application claims priority is also incorporated by reference herein in its entirety in the manner described above for publications and references.  
                           TABLE I                                   Corresponding               Nucleic Acid   Protein       Gene Name   GSK Gene ID   SEQ ID NO&#39;s   SEQ ID NO&#39;s                  sbg237163LIPASE   237163   SEQ ID NO: 1   SEQ ID NO: 23       sbg251170CEAa   251170   SEQ ID NO: 2   SEQ ID NO: 24               SEQ ID NO: 3   SEQ ID NO: 25       sbg389686WNT15a   389686   SEQ ID NO: 4   SEQ ID NO: 26               SEQ ID NO: 5   SEQ ID NO: 27       sbg236015LIPASE   236015   SEQ ID NO: 6   SEQ ID NO: 28               SEQ ID NO: 7   SEQ ID NO: 29       sbg417005LAMININ_ALPHA   417005   SEQ ID NO: 8   SEQ ID NO: 30               SEQ ID NO: 9   SEQ ID NO: 31       sbg425649KINASEa   425649   SEQ ID NO: 10   SEQ ID NO: 32       sbg419582PROTOCADHERIN   419582   SEQ ID NO: 11   SEQ ID NO: 33               SEQ ID NO: 12   SEQ ID NO: 34       sbg453915TECTORINa   453915   SEQ ID NO: 13   SEQ ID NO: 35       SBh385630.antiinflam   385630   SEQ ID NO: 14   SEQ ID NO: 36               SEQ ID NO: 15   SEQ ID NO: 37       sbg471005nAChR   471005   SEQ ID NO: 16   SEQ ID NO: 38       sbg442445PROa   442445   SEQ ID NO: 17   SEQ ID NO: 39       sbg456548CytoRa   456548   SEQ ID NO: 18   SEQ ID NO: 40               SEQ ID NO: 19   SEQ ID NO: 41       sbg456548CytoRa   456548b   SEQ ID NO: 20   SEQ ID NO: 42       sbg442358PROa   442358   SEQ ID NO: 21   SEQ ID NO: 43               SEQ ID NO: 22   SEQ ID NO: 44                  
 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE II 
               
               
                   
               
               
                   
               
               
                   
                   
                   
                   
                 Cell 
               
               
                   
                 Gene 
                 Closest Polynuclotide 
                 Closest Polypeptide by 
                 Localization 
               
               
                 Gene Name 
                 Family 
                 by homology 
                 homology 
                 (by homology) 
               
               
                   
               
             
            
               
                 sbg237163LIPASE 
                 Pancreatic 
                 GB: AC011328 
                 Mouse pancreatic lipase 
                 Secreted 
               
               
                   
                 lipase 
                 Direct submitted (06- 
                 related protein 1, gi: 
               
               
                   
                   
                 OCT-1999) Genome 
                 9256628 
               
               
                   
                   
                 Therapeutics 
                 Remington, S. G., 
               
               
                   
                   
                 Corporation, 100 
                 Lima, P. H. and Nelson, J. D. 
               
               
                   
                   
                 Beaver Street, 
                 Invest. Ophthalmol. Vis. 
               
               
                   
                   
                 Waltham, MA 02453, 
                 Sci. 40 (6), 1081-1090 
               
               
                   
                   
                 USA 
                 (1999) 
               
               
                 sbg251170CEAa 
                 Carcinoembryonic 
                 GB: AC020914 
                 Mouse putative protein, 
                 Secreted 
               
               
                   
                 antigen 
                 Submitted (12-JAN- 
                 gi: 12842545 
               
               
                   
                   
                 2000) Production 
                 Carninci, P., Shibata, Y., 
               
               
                   
                   
                 Sequencing Facility, 
                 Hayatsu, N., Sugahara, Y., 
               
               
                   
                   
                 DOE Joint 
                 Shibata, K., Itoh, M., 
               
               
                   
                   
                 Genome Institute, 2800 
                 Konno, H., Okazaki, Y., 
               
               
                   
                   
                 Mitchell Drive, Walnut 
                 Muramatsu, M. and 
               
               
                   
                   
                 Creek, CA 94598, USA 
                 Hayashizaki, Y. 
               
               
                   
                   
                   
                 Genome Res. 10 (10), 
               
               
                   
                   
                   
                 1617-1630 (2000). 
               
               
                 sbg389686WNT15a 
                 WNT15 
                 GB: AC015855 
                 Chicken WNT14 protein, 
                 Secreted 
               
               
                   
                   
                 Directly submitted (17- 
                 gi: 3915306 
               
               
                   
                   
                 NOV-1999) Whitehead 
                 Bergstein I, Eisenberg L M, 
               
               
                   
                   
                 Institute/MIT Center 
                 Bhalerao J, Jenkins N A, 
               
               
                   
                   
                 for Genome Research, 
                 Copeland N G, Osborne 
               
               
                   
                   
                 320 Charles Street, 
                 M P, Bowcock A M, Brown 
               
               
                   
                   
                 Cambridge, MA 02141, 
                 A M; 1997; Genomics 
               
               
                   
                   
                 USA. 
                 46: 450-8. 
               
               
                 sbg236015LIPASE 
                 Lysosomal 
                 GB: AL358532 
                 Rat lingual lipase, 
                 Secreted 
               
               
                   
                 acid 
                 Directly submitted (15- 
                 gi: 126307 
               
               
                   
                 lipase 
                 DEC-2000) by Sanger 
                 Docherty, A. J., 
               
               
                   
                   
                 Centre, Hinxton, 
                 Bodmer, M. W., Angal, S., 
               
               
                   
                   
                 Cambridgeshire, CB10 
                 Verger, R., Riviere, C., 
               
               
                   
                   
                 1SA, UK. 
                 Lowe, P. A., Lyons, A., 
               
               
                   
                   
                   
                 Emtage, J. S. and Harris, T. J. 
               
               
                   
                   
                   
                 Nucleic Acids Res. 13 (6), 
               
               
                   
                   
                   
                 1891-1903 (1985) 
               
               
                 sbg417005LAMININ_ALPHA 
                 Laminin 
                 GB: AL354836 
                 Human laminin alpha 5, 
                 Secreted 
               
               
                   
                 alpha 
                 Direct submitted (02- 
                 gi: 12274842 
               
               
                   
                   
                 MAY-2000) Sanger 
                 Submitted (14-FEB-2001) 
               
               
                   
                   
                 Centre, Hinxton, 
                 by Sanger Centre, Hinxton, 
               
               
                   
                   
                 Cambridgeshire, CB10 
                 Cambridgeshire, CB10 
               
               
                   
                   
                 1SA 
                 1SA, UK. 
               
               
                 sbg425649KINASEa 
                 Casein 
                 GB: AL356107 
                 Human casein kinase I- 
                 Cytosolic 
               
               
                   
                 kinase I- 
                 Submitted (16-MAY- 
                 alpha, 
               
               
                   
                 alpha 
                 2000) by 
                 gi: 2134872 
               
               
                   
                   
                 Sanger Centre, 
                 Fish, K. J., 
               
               
                   
                   
                 Hinxton, 
                 Cegielska, A., 
               
               
                   
                   
                 Cambridgeshire, CB10 
                 Getman, M. E., 
               
               
                   
                   
                 1SA, UK. 
                 Landes, G. M. and 
               
               
                   
                   
                   
                 Virshup, D. M. 
               
               
                   
                   
                   
                 J. Biol. Chem. 270 (25), 
               
               
                   
                   
                   
                 14875-14883 (1995) 
               
               
                 sbg419582PROTOCADHERIN 
                 Protocadherin 
                 GB: AL355593 
                 Human protocadherin 68 
                 Secreted 
               
               
                   
                   
                 Direct submitted (17- 
                 gi: 11433373 
               
               
                   
                   
                 MAY-2000) Sanger 
                 Submitted (16-NOV-2000) 
               
               
                   
                   
                 Centre, Hinxton, 
                 by National Center for 
               
               
                   
                   
                 Cambridgeshire, CB10 
                 Biotechnology 
               
               
                   
                   
                 1SA, UK. 
                 Information, NIH, 
               
               
                   
                   
                   
                 Bethesda, MD 20894, USA 
               
               
                 sbg453915TECTORINa 
                 Tectorin 
                 SC:AL157786 
                 Mouse tectorin beta, 
                 Secreted 
               
               
                   
                 Beta 
                 Submitted (04-MAY- 
                 gi: 7363457 
               
               
                   
                   
                 2001) by Sanger 
                 Legan, P. K., Rau, A., 
               
               
                   
                   
                 Centre, Hinxton, 
                 Keen, J. N. and 
               
               
                   
                   
                 Cambridgeshire, CB10 
                 Richardson, G. P. 
               
               
                   
                   
                 1SA, UK. 
                 J. Biol. Chem. 272 (13), 
               
               
                   
                   
                   
                 8791-8801 (1997) 
               
               
                 SBh385630.antiinflam 
                 Lipase 
                 GB: AC015525 
                 Rabbit lacrimal lipase, 
                 Secreted 
               
               
                   
                   
                 Submitted (16-NOV- 
                 gi: 13560884 
               
               
                   
                   
                 1999) by Whitehead 
                 Submitted (20-FEB-2001) 
               
               
                   
                   
                 Institute/MIT Center 
                 Ophthalmology, Regions 
               
               
                   
                   
                 for Genome Research, 
                 Hospital, 640 Jackson 
               
               
                   
                   
                 320 Charles Street, 
                 Street, St. Paul, MN 55101, 
               
               
                   
                   
                 Cambridge, MA 02141, 
                 USA 
               
               
                   
                   
                 USA 
               
               
                 sbg471005nAChR 
                 Nicotinic 
                 GB: AC060812 
                 Human cholinergic 
                 Membrane- 
               
               
                   
                 acetylcholine 
                 Direct submitted 
                 receptor, nicotinic, alpha 
                 bound 
               
               
                   
                 receptor 
                 (20-APR-2000) 
                 polypeptide 10, 
               
               
                   
                   
                 Whitehead 
                 gi: 11138123 
               
               
                   
                   
                 Institute/MIT 
                 Lustig, L. R., Peng, H., 
               
               
                   
                   
                 Center for 
                 Hiel, H., Yamamoto, T. 
               
               
                   
                   
                 Genome 
                 and Fuchs, P. A. 
               
               
                   
                   
                 Research, 320 
                 Genomics 73 (3), 272- 
               
               
                   
                   
                 Charles Street, 
                 283 (2001) 
               
               
                   
                   
                 Cambridge, MA 
               
               
                   
                   
                 02141, USA 
               
               
                 sbg442445PROa 
                 Leucine rich 
                 GB: AC060234 
                 RIKEN cDNA mouse 
                 Cytosolic 
               
               
                   
                 repeat protein 
                 Submitted 
                 4930442L21 gene 
               
               
                   
                   
                 (20-APR-2000) 
                 Carninci, P., Shibata, Y., 
               
               
                   
                   
                 Genome 
                 Hayatsu, N., 
               
               
                   
                   
                 Therapeutics 
                 Sugahara, Y., Shibata, K., 
               
               
                   
                   
                 Corporation, 100 
                 Itoh, M., Konno, H., 
               
               
                   
                   
                 Beaver Street, 
                 Okazaki, Y., 
               
               
                   
                   
                 Waltham, 
                 Muramatsu, M. and 
               
               
                   
                   
                 MA 02453, USA 
                 Hayashizaki, Y. 
               
               
                   
                   
                   
                 Genome Res. 10 (10), 
               
               
                   
                   
                   
                 1617-1630 (2000) 
               
               
                 sbg456548CytoRa 
                 Cytokine 
                 GB: AL158138 
                 Human IL20 receptor, 
                 Membrane- 
               
               
                   
                 receptor 
                 Submitted (20- 
                 gi: 7657691 
                 bound 
               
               
                   
                   
                 JAN-2001) by 
                 Xie M H, Aggarwal S, 
               
               
                   
                   
                 Sanger Centre, 
                 Ho W H, Foster J, Zhang 
               
               
                   
                   
                 Hinxton, 
                 Z, Stinson J, Wood W I, 
               
               
                   
                   
                 Cambridgeshire, 
                 Goddard A D and Gurney 
               
               
                   
                   
                 CB10 1SA, UK. 
                 A L. 
               
               
                   
                   
                   
                 J. Biol. Chem. 275 (40), 
               
               
                   
                   
                   
                 31335-31339 (2000) 
               
               
                 sbg442358PROa 
                 Leucine rich 
                 GB: AL139099 
                 Human EXMAD-9 
                 Membrane- 
               
               
                   
                 repeat protein 
                 Submitted (23- 
                 geneseqp: AAB27231 
                 bound 
               
               
                   
                   
                 MAY-2000) by 
                 Submitted by INCYTE 
               
               
                   
                   
                 Genoscope - 
                 GENOMICS INC 
               
               
                   
                   
                 Centre National 
                 Application and 
               
               
                   
                   
                 de Sequencage : 
                 publication date: 
               
               
                   
                   
                 BP 191 91006 
                 WO200068380-A2, 16- 
               
               
                   
                   
                 EVRY cedex - 
                 NOV-00 
               
               
                   
                   
                 FRANCE 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE III 
               
               
                   
               
               
                   
               
               
                   
                   
                 Associated 
               
               
                 Gene Name 
                 Uses 
                 Diseases 
               
               
                   
               
             
            
               
                 sbg237163LIPASE 
                 An embodiment of the invention is the use of sbg237163 
                 Cancer, infection, 
               
               
                   
                 LIPASE as replacement enzymes for patients with chronic 
                 autoimmune 
               
               
                   
                 pancreatitis. A close homologue of sbg237163 LIPASE 
                 disorder, 
               
               
                   
                 is pancreatic lipase. Pancreatic lipase hydrolyzes dietary 
                 hematopoietic 
               
               
                   
                 long chain triacylglycerol to free fatty acids and 
                 disorder, wound 
               
               
                   
                 monoacylglycerols in the intestinal lumen (Lowe M E, 
                 healing disorders, 
               
               
                   
                 Rosenblum J L, and Strauss A W; 1989; J Biol Chem 
                 inflammation. 
               
               
                   
                 264: 20042-8). Pancreatic steatorrhea and pancreatic 
               
               
                   
                 diabetes are the dominant symptoms of patients in a 
               
               
                   
                 certain stage of chronic pancreatitis. In this stage, the 
               
               
                   
                 nutritional state is greatly disturbed and hypoglycemia and 
               
               
                   
                 labile infection are involved. Pancreatic enzyme 
               
               
                   
                 replacement therapy is the principal treatment method for 
               
               
                   
                 pancreatic steatorrhea (Nakamura T, Takeuchi T, and 
               
               
                   
                 Tando Y; 1998; Pancreas 16: 329-36. 
               
               
                 sbg251170CEAa 
                 An embodiment of the invention is the use of 
                 Cancer, 
               
               
                   
                 sbg251170CEAa as cell-surface molecules mediating 
                 autoimmune 
               
               
                   
                 cell-specific interactions in normal and neoplastic cells. A 
                 disorders, wound 
               
               
                   
                 close homologue of sbg251170CEAa is 
                 healing disorders, 
               
               
                   
                 carcinoembryonic antigen-related cell adhesion molecule 
                 hematopoietic 
               
               
                   
                 6. Carcinoembryonic antigen-related cell adhesion 
                 disorders and 
               
               
                   
                 molecule 6 is claimed to function as a cell-surface 
                 infection 
               
               
                   
                 molecules mediating cell-specific interactions in normal 
               
               
                   
                 and neoplastic cells (1. Barnett T, Goebel S J, Nothdurft 
               
               
                   
                 M A, Elting J J, Carcinoembryonic antigen family: 
               
               
                   
                 characterization of cDNAs coding for NCA and CEA and 
               
               
                   
                 suggestion of nonrandom sequence variation in their 
               
               
                   
                 conserved loop-domains. Genomics 1988 Jul; 3(1): 59-66. 
               
               
                   
                 2. Inazawa J, Abe T, Inoue K, Misawa S, Oikawa S, 
               
               
                   
                 Nakazato H, Yoshida M C. Regional assignment of 
               
               
                   
                 nonspecific cross-reacting antigen (NCA) of the CEA 
               
               
                   
                 gene family to chromosome 19 at band q13.2. Cytogenet 
               
               
                   
                 Cell Genet 1989; 52(1-2): 28-31). 
               
               
                 sbg389686WNT15a 
                 An embodiment of the invention is the use of 
                 Cancer, infection, 
               
               
                   
                 sbg389686WNT15a in regulation of cell growth and 
                 autoimmune 
               
               
                   
                 differentiation. Close homologues of 
                 disorder, 
               
               
                   
                 sbg389686WNT15a are Wnt proteins. Wnt proteins are 
                 hematopoietic 
               
               
                   
                 involved in critical developmental processes in both 
                 disorder, wound 
               
               
                   
                 vertebrates and invertebrates and are implicated in 
                 healing disorders, 
               
               
                   
                 regulation of cell growth and differentiation in certain 
                 and inflammation 
               
               
                   
                 adult mammalian tissues (Bergstein I, Eisenberg L M, 
               
               
                   
                 Bhalerao J, Jenkins N A, Copeland N G, Osborne M P, 
               
               
                   
                 Bowcock A M, Brown A M; 1997; Genomics 46: 450-8). 
               
               
                   
                 The Wnt gene family consists of at least 15 structurally 
               
               
                   
                 related genes that encode secreted extracellular 
               
               
                   
                 signaling factors. Wnt signaling is involved in many 
               
               
                   
                 mammalian developmental processes, including cell 
               
               
                   
                 proliferation, differentiation and epithelial-mesenchymal 
               
               
                   
                 interactions, through which they contribute to the 
               
               
                   
                 development of tissues and organs such as the limbs, the 
               
               
                   
                 brain, the reproductive tract and the kidney. Evidence 
               
               
                   
                 from tumor expression studies and transgenic animals 
               
               
                   
                 experiments suggests that inappropriate activation of the 
               
               
                   
                 Wnt signaling pathway is a major feature in human 
               
               
                   
                 neoplasia and that oncogenic activation of this pathway 
               
               
                   
                 can occur at many levels. Inappropriate expression of 
               
               
                   
                 the Wnt ligand and Wnt binding proteins have been 
               
               
                   
                 found in a variety of human tumors (Smalley M J, Dale 
               
               
                   
                 T C; 1999; Cancer Metastasis Rev 18: 215-30). 
               
               
                 sbg236015LIPASE 
                 An embodiment of the invention is the use of 
                 Cancer, infection, 
               
               
                   
                 sbg236015LIPASE for treating lipase deficiency. A 
                 autoimmune 
               
               
                   
                 close homologue of sbg236015LIPASE is lysosomal 
                 disorder, 
               
               
                   
                 acid lipase. The lysosomal acid lipase catalyzes the 
                 hematopoietic 
               
               
                   
                 deacylation of triacylglyceryl and cholesteryl ester core 
                 disorder, wound 
               
               
                   
                 lipids of endocytosed low density lipoproteins. This 
                 healing disorders, 
               
               
                   
                 activity is deficient in patients with Wolman disease and 
                 inflammation, 
               
               
                   
                 cholesteryl ester storage disease, which are caused by a 
                 Wolman disease, 
               
               
                   
                 deficiency of lysosomal acid lipase activity, resulting in 
                 and cholesteryl 
               
               
                   
                 massive accumulation of cholesteryl ester and 
                 ester storage 
               
               
                   
                 triglycerides (Anderson R A, Sando G N; 1991; J Biol 
                 disease 
               
               
                   
                 Chem 266: 22479-84). 
               
               
                 sbg417005LAMININ_ALPHA 
                 An embodiment of the invention is the use of 
                 Cancer, infection, 
               
               
                   
                 sbg417005LAMININ_ALPHA to promote myogenesis 
                 autoimmune 
               
               
                   
                 in skeletal muscle, outgrowth of neurites from central 
                 disorder, 
               
               
                   
                 and peripheral neurons, and mesenchymal to epithelial 
                 hematopoietic 
               
               
                   
                 transitions in kidney. A close homologue of 
                 disorder, wound 
               
               
                   
                 sbg417005LAMININ_ALPHA is laminin. Laminins 
                 healing disorders, 
               
               
                   
                 trimers, composed of alpha, beta, and gamma chains, are 
                 inflammation, 
               
               
                   
                 components of all basal laminae (BLs) throughout the 
                 congenital 
               
               
                   
                 bodies. In mammals they play at least three essential 
                 muscular 
               
               
                   
                 roles. First, they are major structural elements of BLs, 
                 dystrophy, and 
               
               
                   
                 forming one of two self-assembling networks to which 
                 junctional 
               
               
                   
                 other glycoproteins and proteoglycans of the BL attach. 
                 epidermolysis 
               
               
                   
                 Second, they interact with cell surface components such 
                 bullosa 
               
               
                   
                 as dystroglycan to attach cells to the extracellular 
               
               
                   
                 matrix. Third, they are signaling molecules that interact 
               
               
                   
                 with cellular receptors such as the integrins to convey 
               
               
                   
                 important information to the cell interior. The alpha 
               
               
                   
                 chains are ligands for most cellular laminin receptors. 
               
               
                   
                 (Miner J H, Patton B L, Lentz S I, Gilbert D J, Snider W D, 
               
               
                   
                 Jenkins N A, Copeland N G, Sanes J R; 1997; J Cell Biol 
               
               
                   
                 137: 685-701). 
               
               
                 sbg425649KINASEa 
                 An embodiment of the invention is the use of 
                 Cancer, wound 
               
               
                   
                 sbg425649KINASEa in DNA replication and repair, 
                 healing disorders, 
               
               
                   
                 membrane trafficking, neuroprotective, cytostatic, 
                 autoimmune 
               
               
                   
                 cardioactive, immunomodulatory, muscular, vulnerary, 
                 disorders, 
               
               
                   
                 gastrointestinal, nephrotropic, anti-infective, 
                 hematopoietic 
               
               
                   
                 gynaecological and antibacterial activities, and can be 
                 disorders and 
               
               
                   
                 used in gene therapy. Close homologues of 
                 infection 
               
               
                   
                 sbg425649KINASEa is mammalian casein kinases I 
               
               
                   
                 (CKI) and human prostate cancer associated protein. 
               
               
                   
                 CKI belongs to a family of serine/threonine protein 
               
               
                   
                 kinases involved in diverse cellular processes including 
               
               
                   
                 DNA replication and repair, membrane trafficking, 
               
               
                   
                 circadian rhythms and Wnt signaling. Human prostate 
               
               
                   
                 cancer associated proteins have neuroprotective, 
               
               
                   
                 cytostatic, cardioactive, immunomodulatory, muscular, 
               
               
                   
                 vulnerary, gastrointestinal, nephrotropic, anti-infective, 
               
               
                   
                 gynaecological and antibacterial activities, and can be 
               
               
                   
                 used in gene therapy. 
               
               
                 sbg419582PROTOCADHERIN 
                 An embodiment of the invention is the use of 
                 Cancer, infection, 
               
               
                   
                 sbg419582PROTOCADHERIN in functional systems of 
                 autoimmune 
               
               
                   
                 the nervous system, and may be involved in the 
                 disorder, 
               
               
                   
                 formation of the neural network. A close homologue of 
                 hematopoietic 
               
               
                   
                 sbg419582PROTOCADHERIN is protocadherin. The 
                 disorder, wound 
               
               
                   
                 expression of protocadherin is developmentally 
                 healing disorders, 
               
               
                   
                 regulated in a subset of the functional systems of the 
                 inflammation, 
               
               
                   
                 nervous system, and may be involved in the formation 
                 Parkinson&#39;s 
               
               
                   
                 of the neural network by segregation of the brain nuclei 
                 disease, 
               
               
                   
                 and mediation of the axonal connections (Hirano S, Yan 
                 Huntington&#39;s 
               
               
                   
                 Q, Suzuki S T; 1999; J Neurosci 19: 995-1005). The 
                 chorea, and 
               
               
                   
                 members of the cadherin superfamily are divided into 
                 multiple sclerosis 
               
               
                   
                 two groups: classical cadherin type and protocadherin 
               
               
                   
                 type. The current cadherins appear to have evolved from 
               
               
                   
                 protocadherin (Suzuki S T; 1996; J Cell Sci 109: 2609- 
               
               
                   
                 11). 
               
               
                 sbg453915TECTORINa 
                 An embodiment of the invention is the use of 
                 Infection, cancer, 
               
               
                   
                 sbg453915TECTORINa, a secreted protein, in cellular 
                 wound healing 
               
               
                   
                 adhesion. A close homologue of 
                 disorders, 
               
               
                   
                 sbg453915TECTORINa is mouse tectorin beta. The 
                 hemotopoietic 
               
               
                   
                 beta-tectorin is a protein of 36,074 Da that contains 4 
                 disorders and 
               
               
                   
                 consensus N glycosylation sites and a single zona 
                 autoimmune 
               
               
                   
                 pellucida domain. It is similar to components of the 
                 disorders. 
               
               
                   
                 sperm-egg adhesion system, and, as such may have a 
               
               
                   
                 similar functional role (Legan P K, Rau A, Keen J N, 
               
               
                   
                 Richardson G P, The mouse tectorins. Modular matrix 
               
               
                   
                 proteins of the inner ear homologous to components of 
               
               
                   
                 the sperm-egg adhesion system. J Biol Chem 1997 Mar 
               
               
                   
                 28; 272(13): 8791-801). 
               
               
                 SBh385630.antiinflam 
                 An embodiment of the invention is the use of 
                 Lematopoietic 
               
               
                   
                 SBh385630.antiinflam in gene therapy and are also 
                 disorders, wound 
               
               
                   
                 suggested to have cytokine and cell 
                 healing disorders, 
               
               
                   
                 proliferation/differentiation activity, immune 
                 viral and bacterial 
               
               
                   
                 stimulating (e.g. vaccines) or suppressing activity, 
                 infections, cancer, 
               
               
                   
                 haematopoiesis regulating activity, tissue growth 
                 and autoimmune 
               
               
                   
                 activity, activin/inhibinactivity, 
                 diseases 
               
               
                   
                 chemotactic/chemokinetic activity, haemostatic and 
               
               
                   
                 thrombolytic activity, receptor/ligand activity, anti- 
               
               
                   
                 inflammatory activity, cadherin/tumour invasion 
               
               
                   
                 suppressor activity, and tumour inhibition activity. 
               
               
                   
                 Lipases are also reported to be useful for gene therapy 
               
               
                   
                 (WO9957132-A1;. Agostino, M. J., filed by GENETICS 
               
               
                   
                 INST INC.). Close homologues of 
               
               
                   
                 SBh385630.antiinflam include lipases. 
               
               
                 sbg471005nAChR 
                 An embodiment of the invention is the use of 
                 Cancer, infection, 
               
               
                   
                 sbg471005nAChR in physiological and behavioural 
                 autoimmune 
               
               
                   
                 processes of the brain. A close homologue of 
                 disorder, 
               
               
                   
                 sbg471005nAChR is neuronal nicotinic acetylcholine 
                 hematopoietic 
               
               
                   
                 receptors. Neuronal nicotinic acetylcholine receptors 
                 disorder, wound 
               
               
                   
                 are a family of ion channels which are widely 
                 healing disorders, 
               
               
                   
                 distributed in the human brain. There are many 
                 inflammation, 
               
               
                   
                 subtypes, and each has individual pharmacological and 
                 Alzheimer&#39;s 
               
               
                   
                 functional profiles. They mediate the effects of nicotine, 
                 disease, 
               
               
                   
                 and are involved in a number of physiological and 
                 Parkinson&#39;s 
               
               
                   
                 behavioural processes. Additionally they may be 
                 disease, and 
               
               
                   
                 implicated in a number of pathological conditions such 
                 schizophrenia 
               
               
                   
                 as Alzheimer&#39;s disease, Parkinson&#39;s disease and 
               
               
                   
                 schizophrenia (Paterson D, Nordberg A; 2000; Prog 
               
               
                   
                 Neurobiol 61: 75-111). 
               
               
                 sbg442445PROa 
                 An embodiment of the invention is the use of 
                 Inflammation, 
               
               
                   
                 sbg442445PROa which may be involved in protein- 
                 autoimmune 
               
               
                   
                 protein interation and signal transduction in immune 
                 disorders, asthma, 
               
               
                   
                 system. sbg442445PROa was expressed predominantly 
                 allergies 
               
               
                   
                 in lung and spleen/lymph. It encodes a protein with 
                 and 
               
               
                   
                 leucine rich repeats which may be involved in protein- 
                 sbg442445PROa-associated 
               
               
                   
                 protein interation and signal transduction in immune 
                 disorders 
               
               
                   
                 systems. 
               
               
                 sbg456548CytoRa 
                 The present gene has been cloned. Sybrman data 
                 Chronic and acute 
               
               
                   
                 showed its high expression levels in placenta and 
                 inflammation, 
               
               
                   
                 moderate levels in spleen and lymph. A close 
                 allergy, arthritis 
               
               
                   
                 homologue of sbg456548CytoRa is another Class II 
                 (including 
               
               
                   
                 cytokine receptor, ZCYTOR7. An embodiment of the 
                 rheumatoid 
               
               
                   
                 invention is the use of sbg456548CytoRa, a decoy 
                 arthritis), 
               
               
                   
                 receptor, in the identification of other ligands, the 
                 septicemia, 
               
               
                   
                 promotion of anti-microbial activation of these cells, 
                 autoimmune 
               
               
                   
                 and/or potentiate the effectiveness of the natural ligand. 
                 diseases (e.g., 
               
               
                   
                 Growth factors are known to promote the progression of 
                 inflammatory 
               
               
                   
                 cancer. A decoy receptor could interfere with that 
                 bowel disease, 
               
               
                   
                 process. Proliferation, survival and differentiation can 
                 psoriasis), 
               
               
                   
                 be transduced from activated cytokine receptors (Cell 
                 transplant 
               
               
                   
                 Signal. 1998. 10(9): 619-628). Blocking these events 
                 rejection, graft vs. 
               
               
                   
                 could be crucial in modulating various diseases. 
                 host disease, 
               
               
                   
                 The decoy receptor could potentially interfere with 
                 infection, stroke, 
               
               
                   
                 binding of these or other putative ligands, preventing 
                 ischemia, acute 
               
               
                   
                 downstream effects (Blood. 1999. 94(6): 1943-1951). 
                 respiratory disease 
               
               
                   
                 GM-CSF also has anti-apoptotic activity. A decoy 
                 syndrome, asthma, 
               
               
                   
                 receptor might then be able to block GM-CSF&#39;s anti- 
                 restenosis, brain 
               
               
                   
                 apoptotic actions when appropriate (Mol Biol Cell. 
                 injury, AIDS, bone 
               
               
                   
                 1999. 10(11): 3959-3970). Roles for blocking the 
                 diseases, cancer, 
               
               
                   
                 activity of the decoy receptor can be envisioned. GM- 
                 atheroschlerosis, 
               
               
                   
                 CSF promotes anti-microbial functions of mature 
                 Alzheimers 
               
               
                   
                 neutrophils. Inhibiting the activity of an interfering 
                 disease,, 
               
               
                   
                 decoy receptor could promote anti-microbial activation 
                 hematopoietic 
               
               
                   
                 of these cells. Furthermore, rhGM-CSF is in wide 
                 disorder, and 
               
               
                   
                 clinical use to fight acute myeloid leukemia 
                 wound healing 
               
               
                   
                 (Haematologica. 1991. 82(2): 239-245). Inhibition of a 
                 disorder 
               
               
                   
                 decoy receptor could potentiate the effectiveness of the 
               
               
                   
                 natural ligand. 
               
               
                 sbg442358PROa 
                 An embodiment of the invention is the use of 
                 Cancer, 
               
               
                   
                 sbg442358PROa useful in the prevention and treatment 
                 autoimmune 
               
               
                   
                 of cancers, cell proliferation, cardiovascular, 
                 disorders, 
               
               
                   
                 reproductive, immune, musculoskeletal, developmental 
                 hemotopoietic 
               
               
                   
                 and gastrointestinal disorders and inflammation. Close 
                 disorders, wound 
               
               
                   
                 homologues of sbg442358PROa are human protein 
                 healing disorders 
               
               
                   
                 B27231 and  Drosophila  LRR47 that also contains 
                 and infections 
               
               
                   
                 leucine-rich repeats (LRRs) motifs. LRR has been 
               
               
                   
                 found in a variety of extracellular, membrane and 
               
               
                   
                 cytoplasmic proteins.and are believed to mediate 
               
               
                   
                 specific protein-protein interactions and to function in 
               
               
                   
                 cellular adhesion (Ntwasa, M., Buchanan, S. G. and 
               
               
                   
                 Gay, N. J. Biochim. Biophys. Acta 1218 (2), 181-186 
               
               
                   
                 (1994)). 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE IV 
               
               
                   
               
               
                   
               
               
                 Quantitative, Tissue-specific mRNA expression detected using SybrMan 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Quantitative, tissue-specific, mRNA expression patterns of the genes were measured using SYBR-Green Quantitative 
               
               
                 PCR (Applied Biosystems, Foster City, CA; see Schmittgen T. D. et al., Analytical Biochemistry 285: 194-204, 2000) 
               
               
                 and human cDNAs prepared from various human tissues. Gene-specific PCR primers were designed using the first 
               
               
                 nucleic acid sequence listed in the Sequence List for each gene. Results are presented as the number of copies of 
               
               
                 each specific gene&#39;s mRNA detected in 1 ng mRNA pool from each tissue. Two replicate mRNA measurements were 
               
               
                 made from each tissue RNA. 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg237163LIPASE 
               
            
           
           
               
               
            
               
                   
                 Tissue-Specific mRNA Expression 
               
               
                   
                 (copies per ng mRNA; avg. ± range for 2 data points per tissue) 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                 Skeletal 
                   
                   
                   
                   
               
               
                 Gene Name 
                 Brain 
                 Heart 
                 Lung 
                 Liver 
                 Kidney 
                 muscle 
                 Intestine 
                 Spleen/lymph 
                 Placenta 
                 Testis 
               
               
                   
               
               
                 sbg237163LIPASE 
                 5 ± 0 
                 8 ± 2 
                 7 ± 2 
                 −6 ± 1 
                 5 ± 1 
                 5 ± 2 
                 4 ± 6 
                 3 ± 2 
                 1 ± 1 
                 47 ± 1 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg251170CEAa 
               
            
           
           
               
               
            
               
                   
                 Tissue-Specific mRNA Expression 
               
               
                   
                 (copies per ng mRNA; avg. ± range for 2 data points per tissue) 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                 Skeletal 
                   
               
               
                 Gene Name 
                 Brain 
                 Heart 
                 Lung 
                 Liver 
                 Kidney 
                 muscle 
                 Intestine 
               
               
                   
               
               
                 sbg251170CEAa 
                 3 ± 1 
                 19 ± 1 
                 30 ± 5 
                 −5 ± 3 
                 3 ± 1 
                 5 ± 5 
                 21 ± 2 
               
               
                   
               
            
           
           
               
               
            
               
                   
                 Tissue-Specific mRNA Expression 
               
               
                   
                 (copies per ng mRNA; avg. ± range for 2 data points per tissue) 
               
            
           
           
               
               
               
               
            
               
                 Gene Name 
                 Spleen/lymph 
                 Placenta 
                 Testis 
               
               
                   
               
               
                 sbg237163LIPASE 
                 33 ± 4 
                 22 ± 3 
                 14 ± 0 
               
               
                   
               
            
           
           
               
            
               
                 In each gene&#39;s first subset table, two replicate measurements of gene of identification (GOI) mRNA were measured from 
               
               
                 various human tissues (column 2 and 3). The average GOI mRNA copies of the two replicates were made from each 
               
               
                 tissue RNA (column 4). The average amount of 18S rRNA from each tissue RNA was measured (column 5) and used for 
               
               
                 normalization. To make each tissue with the same amount of 50 ng of 18S rRNA, the normalization factor (column 6) 
               
               
                 was calculated by dividing 50 ng with the amount of 18S rRNA measured from each tissue (column 5). The mRNA 
               
               
                 copies per 50 ng of total RNA were obtained by multipling each GOI normalization factor and average mRNA copies 
               
               
                 (column7). 
               
               
                 Fold changes shown in each gene&#39;s second subset table were only calculated for disease tissues which have a 
               
               
                 normal counterpart. There are blanks in the fold change column for all samples that do not have counterparts. 
               
               
                 In addition, the fold change calculations are the fold change in the disease sample as compared to the normal 
               
               
                 sample. Accordingly, there will not be a fold change calculation next to any of the normal samples. For patient 
               
               
                 matched cancer pairs (colon, lung, and breast), each tumor is compared to its specific normal counterpart. When 
               
               
                 patient-matched normal/disease pairs do not exist, each disease sample was compared back to the average of all 
               
               
                 the normal samples of that same tissue type. For example, normal brain from the same patient that provided 
               
               
                 Alzheimer&#39;s brain is not applicable. Three normal brain samples and 4 Alzheimer&#39;s brain samples are used in 
               
               
                 the fold change. Three normal samples were averaged, and each of the Alzheimer&#39;s samples was compared back to 
               
               
                 that average. 
               
               
                   
               
               
                 Abbreviations 
               
               
                   
               
            
           
           
               
               
            
               
                 ALZ 
                 Alzheimer&#39;s Disease 
               
               
                 CT 
                 CLONTECH (1020 East Meadow Circle Palo Alto, CA 
               
               
                   
                 94303-4230, USA) 
               
               
                 KC 
                 Sample prepared by GSK investigator 
               
               
                 COPD 
                 chronic obstructive pulmonary disease 
               
               
                 endo 
                 endothelial 
               
               
                 VEGF 
                 vascular endothelial growth factor 
               
               
                 bFGF 
                 basic fibroblast growth factor 
               
               
                 BM 
                 bone marrow 
               
               
                 osteo 
                 osteoblast 
               
               
                 OA 
                 osteoarthritis 
               
               
                 RA 
                 rheumatoid arthritis 
               
               
                 PBL 
                 peripheral blood lymphocytes 
               
               
                 PBMNC 
                 peripheral blood mononuclear cells 
               
               
                 HIV 
                 human immunodeficiency virus 
               
               
                 HSV 
                 Herpes simplex virus 
               
               
                 HPV 
                 human papilloma virus 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg389686WNT15a 
               
            
           
           
               
            
               
                 Strong expression in Brain and dendritic cells. Brain expression may be from presence of glial cells. Expression 
               
               
                 in RA and OA synovium along with dendritic cells suggests a role for this protein in these diseases. Down 
               
               
                 regulation in ischemic and dilated heart indicates that replacement of protein could be therapeutic. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 sbg389686WNT15a 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 0.00 
                 0.00 
                 0.00 
                 3.06 
                 16.34 
                 0.00 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 0.00 
                 1.71 
                 0.86 
                 0.96 
                 52.36 
                 44.76 
               
               
                 Zenbio 
               
               
                 Adrenal Gland Clontech 
                 2.29 
                 4.18 
                 3.24 
                 0.61 
                 81.97 
                 265.16 
               
               
                 Whole Brain Clontech 
                 698.52 
                 625.01 
                 661.77 
                 7.24 
                 6.91 
                 4570.20 
               
               
                 Fetal Brain Clontech 
                 4.14 
                 6.78 
                 5.46 
                 0.48 
                 103.95 
                 567.57 
               
               
                 Cerebellum Clontech 
                 2.02 
                 3.63 
                 2.83 
                 2.17 
                 23.04 
                 65.09 
               
               
                 Cervix 
                 3.16 
                 10.14 
                 6.65 
                 2.42 
                 20.66 
                 137.40 
               
               
                 Colon 
                 2.48 
                 3.44 
                 2.96 
                 2.71 
                 18.45 
                 54.61 
               
               
                 Endometrium 
                 2.69 
                 5.20 
                 3.95 
                 0.73 
                 68.21 
                 269.10 
               
               
                 Esophagus 
                 10.67 
                 3.24 
                 6.96 
                 1.37 
                 36.50 
                 253.83 
               
               
                 Heart Clontech 
                 9.26 
                 6.07 
                 7.67 
                 1.32 
                 37.88 
                 290.34 
               
               
                 Hypothalamus 
                 7.10 
                 5.16 
                 6.13 
                 0.32 
                 155.28 
                 951.86 
               
               
                 Ileum 
                 2.04 
                 10.37 
                 6.21 
                 2.58 
                 19.38 
                 120.25 
               
               
                 Jejunum 
                 36.78 
                 27.16 
                 31.97 
                 6.60 
                 7.58 
                 242.20 
               
               
                 Kidney 
                 16.46 
                 16.55 
                 16.51 
                 2.12 
                 23.58 
                 389.27 
               
               
                 Liver 
                 14.07 
                 3.34 
                 8.71 
                 1.50 
                 33.33 
                 290.17 
               
               
                 Fetal Liver Clontech 
                 4.60 
                 8.89 
                 6.75 
                 10.40 
                 4.81 
                 32.43 
               
               
                 Lung 
                 3.11 
                 10.49 
                 6.80 
                 2.57 
                 19.46 
                 132.30 
               
               
                 Mammary Gland 
                 3.28 
                 10.61 
                 6.95 
                 13.00 
                 3.85 
                 26.71 
               
               
                 Clontech 
               
               
                 Myometrium 
                 1.79 
                 13.84 
                 7.82 
                 2.34 
                 21.37 
                 166.99 
               
               
                 Omentum 
                 1.96 
                 2.65 
                 2.31 
                 3.94 
                 12.69 
                 29.25 
               
               
                 Ovary 
                 4.50 
                 1.71 
                 3.11 
                 4.34 
                 11.52 
                 35.77 
               
               
                 Pancreas 
                 3.40 
                 2.41 
                 2.91 
                 0.81 
                 61.80 
                 179.54 
               
               
                 Head of Pancreas 
                 2.22 
                 4.63 
                 3.43 
                 1.57 
                 31.85 
                 109.08 
               
               
                 Parotid Gland 
                 5.48 
                 2.07 
                 3.78 
                 5.48 
                 9.12 
                 34.44 
               
               
                 Placenta Clontech 
                 15.15 
                 12.80 
                 13.98 
                 5.26 
                 9.51 
                 132.84 
               
               
                 Prostate 
                 3.39 
                 7.44 
                 5.42 
                 3.00 
                 16.67 
                 90.25 
               
               
                 Rectum 
                 2.98 
                 3.94 
                 3.46 
                 1.23 
                 40.65 
                 140.65 
               
               
                 Salivary Gland 
                 3.24 
                 1.61 
                 2.43 
                 7.31 
                 6.84 
                 16.59 
               
               
                 Clontech 
               
               
                 Skeletal Muscle 
                 2.01 
                 1.55 
                 1.78 
                 1.26 
                 39.68 
                 70.63 
               
               
                 Clontech 
               
               
                 Skin 
                 2.69 
                 3.45 
                 3.07 
                 1.21 
                 41.32 
                 126.86 
               
               
                 Small Intestine 
                 5.39 
                 1.67 
                 3.53 
                 0.98 
                 51.07 
                 180.29 
               
               
                 Clontech 
               
               
                 Spleen 
                 3.96 
                 2.52 
                 3.24 
                 4.92 
                 10.16 
                 32.93 
               
               
                 Stomach 
                 1.08 
                 5.33 
                 3.21 
                 2.73 
                 18.32 
                 58.70 
               
               
                 Testis Clontech 
                 3.27 
                 2.88 
                 3.08 
                 0.57 
                 87.87 
                 270.21 
               
               
                 Thymus Clontech 
                 5.43 
                 4.42 
                 4.93 
                 9.89 
                 5.06 
                 24.90 
               
               
                 Thyroid 
                 2.32 
                 3.01 
                 2.67 
                 2.77 
                 18.05 
                 48.10 
               
               
                 Trachea Clontech 
                 1.64 
                 4.25 
                 2.95 
                 9.71 
                 5.15 
                 15.16 
               
               
                 Urinary Bladder 
                 3.63 
                 6.81 
                 5.22 
                 5.47 
                 9.14 
                 47.71 
               
               
                 Uterus 
                 31.55 
                 11.10 
                 21.33 
                 5.34 
                 9.36 
                 199.67 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                 Mean 
                 copies of mRNA 
                   
                 Fold Change in 
               
               
                 Sample 
                 (GSK 
                 GOI 
                 detected/50 ng 
                   
                 Disease 
               
               
                 sbg389686WNT15a 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 36.16 
                 72.32 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 71.5 
                 143.00 
                 colon tumor 
                 1.977323009 
               
               
                 colon normal GW98-178 
                 22080 
                 2.09 
                 4.18 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 9.84 
                 19.68 
                 colon tumor 
                 4.708133971 
               
               
                 colon normal GW98-561 
                 23514 
                 13.09 
                 26.18 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 15.11 
                 30.22 
                 colon tumor 
                 1.154316272 
               
               
                 colon normal GW98-894 
                 24691 
                 8.62 
                 17.24 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 5.76 
                 11.52 
                 colon tumor 
                 −1.496527778 
               
               
                 lung normal GW98-3 
                 20742 
                 140.19 
                 280.38 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 1.67 
                 3.34 
                 lung tumor 
                 −83.94610778 
               
               
                 lung normal GW97-179 
                 20677 
                 60.54 
                 121.08 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 135.62 
                 271.24 
                 lung tumor 
                 2.240171787 
               
               
                 lung normal GW98-165 
                 21922 
                 257.96 
                 515.92 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 61.69 
                 123.38 
                 lung tumor 
                 −4.181552926 
               
               
                 lung normal GW98-282 
                 22584 
                 49.3 
                 98.60 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 12.39 
                 24.78 
                 lung tumor 
                 −3.979015335 
               
               
                 breast normal GW00-392 
                 28750 
                 71.94 
                 71.94 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 41.4 
                 82.80 
                 breast tumor 
                 1.150959133 
               
               
                 breast normal GW00-413 
                 28798 
                 19.37 
                 19.37 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 1.13 
                 2.26 
                 breast tumor 
                 −8.57079646 
               
               
                 breast normal GW00- 
                 27592-95 
                 8.19 
                 8.19 
                 breast 
               
               
                 235:238 
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00- 
                 27588-91 
                 38.27 
                 38.27 
                 breast tumor 
                 4.672771673 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 77.26 
                 154.52 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 37.57 
                 75.14 
                 breast tumor 
                 −2.056428001 
               
               
                 brain normal BB99-542 
                 25507 
                 597.17 
                 1194.34 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 104.34 
                 208.68 
                 brain normal 
               
               
                 brain normalBB99-904 
                 25546 
                 282.15 
                 564.30 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 84.26 
                 168.52 
                 brain stage 5 
                 −3.891367988 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 247.01 
                 494.02 
                 brain stage 5 
                 −1.327422641 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 173.02 
                 346.04 
                 brain stage 5 
                 −1.895079567 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 253.73 
                 507.46 
                 brain stage 5 
                 −1.292266057 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 146.22 
                 292.44 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 150.46 
                 150.46 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 0 
                 0.00 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 4.76 
                 4.76 
                 lung 24 
                 −23.36292017 
               
               
                 lung 28 KC 
                 COPD 
                 10.06 
                 10.06 
                 lung 28 
                 −11.05442346 
               
               
                 lung 23 KC 
                 COPD 
                 2.75 
                 2.75 
                 lung 23 
                 −40.43909091 
               
               
                 lung 25 KC 
                 COPD 
                 1.93 
                 1.93 
                 lung 25 
               
               
                 asthmatic lung 
                 29321 
                 20.88 
                 20.88 
                 asthmatic 
                 −5.326029693 
               
               
                 ODO3112 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29323 
                 133.29 
                 266.58 
                 asthmatic 
                 2.397140481 
               
               
                 ODO3433 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29322 
                 322.77 
                 645.54 
                 asthmatic 
                 5.804824315 
               
               
                 ODO3397 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29325 
                 43.52 
                 87.04 
                 asthmatic 
                 −1.277659697 
               
               
                 ODO4928 
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 1.89 
                 1.89 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 0 
                 0.00 
                 endo VEGF 
                 −1.89 
               
               
                 endo bFGF KC 
                   
                 1.17 
                 1.17 
                 endo bFGF 
                 −1.615384615 
               
               
                 heart Clontech 
                 normal 
                 153.9 
                 307.80 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 137.74 
                 275.48 
                 heart T-1 
                 −1.117322492 
               
               
                 heart (T-14) non- 
                 29422 
                 87.79 
                 175.58 
                 heart T-14 
                 −1.753047044 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399) DCM 
                 29426 
                 43.68 
                 87.36 
                 heart T-3399 
                 −3.523351648 
               
               
                 adenoid GW99-269 
                 26162 
                 17.62 
                 35.24 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 52.34 
                 104.68 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 8.45 
                 16.90 
                 T cells 
               
               
                 PBMNC KC 
                   
                 1.99 
                 1.99 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 4.74 
                 9.48 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 7.65 
                 15.30 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 194.97 
                 389.94 
                 dendritic 
               
               
                   
                   
                   
                   
                 cells 
               
               
                 neutrophils 
                 28440 
                 2.13 
                 2.13 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 7.25 
                 14.50 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 0 
                 0.00 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 0 
                 0.00 
                 BM stim 
                 0 
               
               
                 osteo dif KC 
                   
                 1.48 
                 1.48 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 7.41 
                 7.41 
                 osteo undif 
                 5.006756757 
               
               
                 chondrocytes 
                   
                 26.64 
                 66.60 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 476.3 
                 476.30 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 151.36 
                 302.72 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 165.01 
                 330.02 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 84.02 
                 168.04 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 184.75 
                 369.50 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 223.3 
                 446.60 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 72.31 
                 72.31 
                 OA bone (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 10.46 
                 20.92 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 111.79 
                 223.58 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 215.54 
                 431.08 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 81.85 
                 163.70 
                 Cartilage 
                 −2.633353696 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 2.31 
                 4.62 
                 PBL 
               
               
                   
                   
                   
                   
                 unifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 2.28 
                 4.56 
                 PBL HIV 
                 −1.013157895 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected 
                 29158 
                 2.37 
                 4.74 
                 MRC5 
               
               
                 (100%) 
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 37.5 
                 75.00 
                 MRC5 HSV 
                 15.82278481 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 0.93 
                 1.86 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 1.33 
                 2.66 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg389686WNT15a 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 1.98 
               
               
                   
                 colon tumor 
                 4.71 
               
               
                   
                 colon tumor 
                 1.15 
               
               
                   
                 colon tumor 
                 −1.50 
               
               
                   
                 lung tumor 
                 −83.95 
               
               
                   
                 lung tumor 
                 2.24 
               
               
                   
                 lung tumor 
                 −4.18 
               
               
                   
                 lung tumor 
                 −3.98 
               
               
                   
                 breast tumor 
                 1.15 
               
               
                   
                 breast tumor 
                 −8.57 
               
               
                   
                 breast tumor 
                 4.67 
               
               
                   
                 breast tumor 
                 −2.06 
               
               
                   
                 brain stage 5 ALZ 
                 −3.89 
               
               
                   
                 brain stage 5 ALZ 
                 −1.33 
               
               
                   
                 brain stage 5 ALZ 
                 −1.90 
               
               
                   
                 brain stage 5 ALZ 
                 −1.29 
               
               
                   
                 lung 24 
                 −23.36 
               
               
                   
                 lung 28 
                 −11.05 
               
               
                   
                 lung 23 
                 −40.44 
               
               
                   
                 asthmatic lung 
                 −5.33 
               
               
                   
                 asthmatic lung 
                 2.40 
               
               
                   
                 asthmatic lung 
                 5.80 
               
               
                   
                 asthmatic lung 
                 −1.28 
               
               
                   
                 endo VEGF 
                 −1.89 
               
               
                   
                 endo bFGF 
                 −1.62 
               
               
                   
                 heart T-1 
                 −1.12 
               
               
                   
                 heart T-14 
                 −1.75 
               
               
                   
                 heart T-3399 
                 −3.52 
               
               
                   
                 BM stim 
                 0.00 
               
               
                   
                 osteo undif 
                 5.01 
               
               
                   
                 Cartilage (pool) 
                 −2.63 
               
               
                   
                 PBL HIV IIIB 
                 −1.01 
               
               
                   
                 MRC5 HSV strain F 
                 15.82 
               
               
                   
                   
               
            
           
           
               
            
               
                 Gene Name sbg236015LIPASE 
               
            
           
           
               
            
               
                 Strongly expressed in neutrophils and eosinophils suggesting an immune system function. Additional expression 
               
               
                 is seen in RA and OA synovium and1/3 OA bone samples. This suggests an involvement of 236015 in RA and OA. The 
               
               
                 high expression in skin when taken together with expression in neutrophils and eosinophils suggests possible 
               
               
                 involvement in immune pathologies of the skin ie. Eosinophilia, psoriasis and eczema. The expression in 
               
               
                 eosinophils also suggests involvement in allergic reactions. Expression in neutrophils suggests role in 
               
               
                 anti-infectives. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 sbg236015LIPASE 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 0.00 
                 11.45 
                 5.73 
                 3.06 
                 16.34 
                 93.55 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 0.00 
                 1.33 
                 0.67 
                 0.96 
                 52.36 
                 34.82 
               
               
                 Zenbio 
               
               
                 Adrenal Gland Clontech 
                 0.52 
                 5.04 
                 2.78 
                 0.61 
                 81.97 
                 227.87 
               
               
                 Whole Brain Clontech 
                 15.73 
                 14.55 
                 15.14 
                 7.24 
                 6.91 
                 104.56 
               
               
                 Fetal Brain Clontech 
                 1.02 
                 0.94 
                 0.98 
                 0.48 
                 103.95 
                 101.87 
               
               
                 Cerebellum Clontech 
                 0.38 
                 0.39 
                 0.39 
                 2.17 
                 23.04 
                 8.87 
               
               
                 Cervix 
                 16.33 
                 20.03 
                 18.18 
                 2.42 
                 20.66 
                 375.62 
               
               
                 Colon 
                 32.41 
                 50.89 
                 41.65 
                 2.71 
                 18.45 
                 768.45 
               
               
                 Endometrium 
                 0.40 
                 0.42 
                 0.41 
                 0.73 
                 68.21 
                 27.97 
               
               
                 Esophagus 
                 5.45 
                 22.47 
                 13.96 
                 1.37 
                 36.50 
                 509.49 
               
               
                 Heart Clontech 
                 0.92 
                 0.00 
                 0.46 
                 1.32 
                 37.88 
                 17.42 
               
               
                 Hypothalamus 
                 0.50 
                 1.59 
                 1.05 
                 0.32 
                 155.28 
                 162.27 
               
               
                 Ileum 
                 41.95 
                 1.51 
                 21.73 
                 2.58 
                 19.38 
                 421.12 
               
               
                 Jejunum 
                 7.59 
                 15.40 
                 11.50 
                 6.60 
                 7.58 
                 87.08 
               
               
                 Kidney 
                 5.32 
                 6.82 
                 6.07 
                 2.12 
                 23.58 
                 143.16 
               
               
                 Liver 
                 12.64 
                 19.46 
                 16.05 
                 1.50 
                 33.33 
                 535.00 
               
               
                 Fetal Liver Clontech 
                 10.02 
                 5.90 
                 7.96 
                 10.40 
                 4.81 
                 38.27 
               
               
                 Lung 
                 22.86 
                 24.78 
                 23.82 
                 2.57 
                 19.46 
                 463.42 
               
               
                 Mammary Gland 
                 1.53 
                 20.56 
                 11.05 
                 13.00 
                 3.85 
                 42.48 
               
               
                 Clontech 
               
               
                 Myometrium 
                 16.05 
                 1.34 
                 8.70 
                 2.34 
                 21.37 
                 185.79 
               
               
                 Omentum 
                 8.33 
                 9.88 
                 9.11 
                 3.94 
                 12.69 
                 115.55 
               
               
                 Ovary 
                 8.22 
                 14.40 
                 11.31 
                 4.34 
                 11.52 
                 130.30 
               
               
                 Pancreas 
                 0.00 
                 1.58 
                 0.79 
                 0.81 
                 61.80 
                 48.83 
               
               
                 Head of Pancreas 
                 0.00 
                 1.98 
                 0.99 
                 1.57 
                 31.85 
                 31.53 
               
               
                 Parotid Gland 
                 5.30 
                 11.45 
                 8.38 
                 5.48 
                 9.12 
                 76.41 
               
               
                 Placenta Clontech 
                 11.93 
                 1.22 
                 6.58 
                 5.26 
                 9.51 
                 62.50 
               
               
                 Prostate 
                 0.00 
                 0.00 
                 0.00 
                 3.00 
                 16.67 
                 0.00 
               
               
                 Rectum 
                 6.96 
                 1.27 
                 4.12 
                 1.23 
                 40.65 
                 167.28 
               
               
                 Salivary Gland 
                 0.34 
                 0.53 
                 0.44 
                 7.31 
                 6.84 
                 2.98 
               
               
                 Clontech 
               
               
                 Skeletal Muscle 
                 176.88 
                 0.41 
                 88.65 
                 1.26 
                 39.68 
                 3517.66 
               
               
                 Clontech 
               
               
                 Skin 
                 95.17 
                 147.16 
                 121.17 
                 1.21 
                 41.32 
                 5006.82 
               
               
                 Small Intestine 
                 0.35 
                 1.31 
                 0.83 
                 0.98 
                 51.07 
                 42.39 
               
               
                 Clontech 
               
               
                 Spleen 
                 105.73 
                 80.76 
                 93.25 
                 4.92 
                 10.16 
                 947.61 
               
               
                 Stomach 
                 0.56 
                 3.73 
                 2.15 
                 2.73 
                 18.32 
                 39.29 
               
               
                 Testis Clontech 
                 0.79 
                 0.78 
                 0.79 
                 0.57 
                 87.87 
                 68.98 
               
               
                 Thymus Clontech 
                 22.00 
                 22.48 
                 22.24 
                 9.89 
                 5.06 
                 112.44 
               
               
                 Thyroid 
                 0.65 
                 0.48 
                 0.57 
                 2.77 
                 18.05 
                 10.20 
               
               
                 Trachea Clontech 
                 1.20 
                 0.00 
                 0.60 
                 9.71 
                 5.15 
                 3.09 
               
               
                 Urinary Bladder 
                 5.59 
                 8.67 
                 7.13 
                 5.47 
                 9.14 
                 65.17 
               
               
                 Uterus 
                 19.26 
                 27.10 
                 23.18 
                 5.34 
                 9.36 
                 217.04 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                 Mean 
                 copies of mRNA 
                   
                 Fold Change in 
               
               
                 Sample 
                 (GSK 
                 GOI 
                 detected/50 ng 
                   
                 Disease 
               
               
                 sbg236015LIPASE 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 58.7 
                 117.40 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 300.92 
                 601.84 
                 colon tumor 
                 5.126405451 
               
               
                 colon normal GW98-178 
                 22080 
                 8.78 
                 17.56 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 23.74 
                 47.48 
                 colon tumor 
                 2.703872437 
               
               
                 colon normal GW98-561 
                 23514 
                 27.1 
                 54.20 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 39.16 
                 78.32 
                 colon tumor 
                 1.44501845 
               
               
                 colon normal GW98-894 
                 24691 
                 10.15 
                 20.30 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 144.58 
                 289.16 
                 colon tumor 
                 14.24433498 
               
               
                 lung normal GW98-3 
                 20742 
                 165.8 
                 331.60 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 80.9 
                 161.80 
                 lung tumor 
                 −2.049443758 
               
               
                 lung normal GW97-179 
                 20677 
                 37.81 
                 75.62 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 109.72 
                 219.44 
                 lung tumor 
                 2.90187781 
               
               
                 lung normal GW98-165 
                 21922 
                 150.06 
                 300.12 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 169.73 
                 339.46 
                 lung tumor 
                 1.131080901 
               
               
                 lung normal GW98-282 
                 22584 
                 489.42 
                 978.84 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 188.22 
                 376.44 
                 lung tumor 
                 −2.600255021 
               
               
                 breast normal GW00-392 
                 28750 
                 44.86 
                 44.86 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 46.35 
                 92.70 
                 breast tumor 
                 2.06642889 
               
               
                 breast normal GW00-413 
                 28798 
                 16.35 
                 16.35 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 55.98 
                 111.96 
                 breast tumor 
                 6.847706422 
               
               
                 breast normal GW00- 
                 27592-95 
                 3.84 
                 3.84 
                 breast 
               
               
                 235:238 
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00- 
                 27588-91 
                 35.8 
                 35.80 
                 breast tumor 
                 9.322916667 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 12.14 
                 24.28 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 44.85 
                 89.70 
                 breast tumor 
                 3.694398682 
               
               
                 brain normal BB99-542 
                 25507 
                 26.03 
                 52.06 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 14.78 
                 29.56 
                 brain normal 
               
               
                 brain normal BB99-904 
                 25546 
                 3.39 
                 6.78 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 35.71 
                 71.42 
                 brain stage 5 
                 2.423755656 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 9.11 
                 18.22 
                 brain stage 5 
                 −1.617270399 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 8.18 
                 16.36 
                 brain stage 5 
                 −1.801140994 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 46.37 
                 92.74 
                 brain stage 5 
                 3.147285068 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 80.77 
                 161.54 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 233.65 
                 233.65 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 75.27 
                 75.27 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 68.64 
                 68.64 
                 lung 24 
                 −1.876821096 
               
               
                 lung 28 KC 
                 COPD 
                 94.1 
                 94.10 
                 lung 28 
                 −1.369022317 
               
               
                 lung 23 KC 
                 COPD 
                 88.48 
                 88.48 
                 lung 23 
                 −1.455978752 
               
               
                 lung 25 KC 
                 normal 
                 44.84 
                 44.84 
                 lung 25 
               
               
                 asthmatic lung ODO3112 
                 29321 
                 111.42 
                 111.42 
                 asthmatic 
                 −1.156210734 
               
               
                   
                   
                   
                   
                 lung 
               
               
                 asthmatic lung ODO3433 
                 29323 
                 566.5 
                 1133.00 
                 asthmatic 
                 8.794876771 
               
               
                   
                   
                   
                   
                 lung 
               
               
                 asthmatic lung ODO3397 
                 29322 
                 262.77 
                 525.54 
                 asthmatic 
                 4.079487677 
               
               
                   
                   
                   
                   
                 lung 
               
               
                 asthmatic lung ODO4928 
                 29325 
                 367.52 
                 735.04 
                 asthmatic 
                 5.70572482 
               
               
                   
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 3.23 
                 3.23 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 3.41 
                 3.41 
                 endo VEGF 
                 1.055727554 
               
               
                 endo bFGF KC 
                   
                 0 
                 0.00 
                 endo bFGF 
                 −3.23 
               
               
                 heart Clontech 
                 normal 
                 0 
                 0.00 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 35.96 
                 71.92 
                 heart T-1 
                 71.92 
               
               
                 heart (T-14) non- 
                 29422 
                 18.72 
                 37.44 
                 heart T-14 
                 37.44 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399) DCM 
                 29426 
                 37.97 
                 75.94 
                 heart T-3399 
                 75.94 
               
               
                 adenoid GW99-269 
                 26162 
                 14.17 
                 28.34 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 51.21 
                 102.42 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 111.1 
                 222.20 
                 T cells 
               
               
                 PBMNC KC 
                   
                 162.01 
                 162.01 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 90.49 
                 180.98 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 109.71 
                 219.42 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 2.44 
                 4.88 
                 dendritic 
               
               
                   
                   
                   
                   
                 cells 
               
               
                 neutrophils 
                 28440 
                 1110.91 
                 1110.91 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 835.72 
                 1671.44 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 181.05 
                 181.05 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 93.96 
                 93.96 
                 BM stim 
                 −1.92688378 
               
               
                 osteo dif KC 
                   
                 0 
                 0.00 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 0.72 
                 0.72 
                 osteo undif 
                 0.72 
               
               
                 chondrocytes 
                   
                 2.03 
                 5.08 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 27.82 
                 27.82 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 84.94 
                 169.88 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 46.58 
                 93.16 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 248.24 
                 496.48 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 148.32 
                 296.64 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 260.28 
                 520.56 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 10.27 
                 10.27 
                 OA bone 
               
               
                   
                   
                   
                   
                 (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 17.32 
                 34.64 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 657.01 
                 1314.02 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 59.17 
                 118.34 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 23.33 
                 46.66 
                 Cartilage 
                 −2.53621946 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 23.51 
                 47.02 
                 PBL 
               
               
                   
                   
                   
                   
                 unifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 5.86 
                 11.72 
                 PBL HIV 
                 −4.011945392 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected (100%) 
                 29158 
                 3.79 
                 7.58 
                 MRC5 
               
               
                   
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 80.19 
                 160.38 
                 MRC5 HSV 
                 21.15831135 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 95.42 
                 190.84 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 16.18 
                 32.36 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg236015LIPASE 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 5.13 
               
               
                   
                 colon tumor 
                 2.70 
               
               
                   
                 colon tumor 
                 1.45 
               
               
                   
                 colon tumor 
                 14.24 
               
               
                   
                 lung tumor 
                 −2.05 
               
               
                   
                 lung tumor 
                 2.90 
               
               
                   
                 lung tumor 
                 1.13 
               
               
                   
                 lung tumor 
                 −2.60 
               
               
                   
                 breast tumor 
                 2.07 
               
               
                   
                 breast tumor 
                 6.85 
               
               
                   
                 breast tumor 
                 9.32 
               
               
                   
                 breast tumor 
                 3.69 
               
               
                   
                 brain stage 5 ALZ 
                 2.42 
               
               
                   
                 brain stage 5 ALZ 
                 −1.62 
               
               
                   
                 brain stage 5 ALZ 
                 −1.80 
               
               
                   
                 brain stage 5 ALZ 
                 3.15 
               
               
                   
                 lung 24 
                 −1.88 
               
               
                   
                 lung 28 
                 −1.37 
               
               
                   
                 lung 23 
                 −1.46 
               
               
                   
                 asthmatic lung 
                 −1.16 
               
               
                   
                 asthmatic lung 
                 8.79 
               
               
                   
                 asthmatic lung 
                 4.08 
               
               
                   
                 asthmatic lung 
                 5.71 
               
               
                   
                 endo VEGF 
                 1.06 
               
               
                   
                 endo bFGF 
                 −3.23 
               
               
                   
                 heart T-1 
                 71.92 
               
               
                   
                 heart T-14 
                 37.44 
               
               
                   
                 heart T-3399 
                 75.94 
               
               
                   
                 BM stim 
                 −1.93 
               
               
                   
                 osteo undif 
                 0.72 
               
               
                   
                 Cartilage (pool) 
                 −2.54 
               
               
                   
                 PBL HIV IIIB 
                 −4.01 
               
               
                   
                 MRC5 HSV strain F 
                 21.16 
               
               
                   
                   
               
            
           
           
               
            
               
                 Gene Name sbg417005LAMININ 
               
            
           
           
               
            
               
                 Expression in adenoid, tonsil and B-cells with corroborating expression in RA/OA samples and asthmatic lung (1/4) 
               
               
                 suggests involvement in these diseases. Strong expression in brain with overexpression in Alzheimer&#39;s disease 
               
               
                 indicates a role in AD. Down regulation in HSV infected cells suggests potential host cell factor. Expression in 
               
               
                 colon and lung normal/tumor pairs without corroborating expression in normal tissues suggests immune cell 
               
               
                 infiltrates. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 sbg417005LAMININ 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 60.2785303 
                 73.59679955 
                 66.94 
                 3.06 
                 16.34 
                 1093.75 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 3.032572965 
                 1.985862153 
                 2.51 
                 0.96 
                 52.36 
                 131.37 
               
               
                 Zenbio 
               
               
                 Adrenal Gland 
                 0.965703497 
                 0.965703497 
                 0.97 
                 0.61 
                 81.97 
                 79.16 
               
               
                 Clontech 
               
               
                 Whole Brain Clontech 
                 4131.557992 
                 6997.879078 
                 5564.72 
                 7.24 
                 6.91 
                 38430.38 
               
               
                 Fetal Brain Clontech 
                 0.965703497 
                 3.268211325 
                 2.12 
                 0.48 
                 103.95 
                 220.06 
               
               
                 Cerebellum Clontech 
                 3.301057867 
                 17.3966665 
                 10.35 
                 2.17 
                 23.04 
                 238.45 
               
               
                 Cervix 
                 5.920484049 
                 7.517891571 
                 6.72 
                 2.42 
                 20.66 
                 138.83 
               
               
                 Colon 
                 35.48962684 
                 22.53180605 
                 29.01 
                 2.71 
                 18.45 
                 535.25 
               
               
                 Endometrium 
                 11.59757492 
                 0.965703497 
                 6.28 
                 0.73 
                 68.21 
                 428.49 
               
               
                 Esophagus 
                 7.098528857 
                 3.523216475 
                 5.31 
                 1.37 
                 36.50 
                 193.83 
               
               
                 Heart Clontech 
                 0.965703497 
                 5.368977287 
                 3.17 
                 1.32 
                 37.88 
                 119.98 
               
               
                 Hypothalamus 
                 0.965703497 
                 0.965703497 
                 0.97 
                 0.32 
                 155.28 
                 149.95 
               
               
                 Ileum 
                 30.81006847 
                 14.15032296 
                 22.48 
                 2.58 
                 19.38 
                 435.66 
               
               
                 Jejunum 
                 44.08994058 
                 30.29386314 
                 37.19 
                 6.60 
                 7.58 
                 281.76 
               
               
                 Kidney 
                 9.424973981 
                 15.68529125 
                 12.56 
                 2.12 
                 23.58 
                 296.11 
               
               
                 Liver 
                 3.742288161 
                 0.965703497 
                 2.35 
                 1.50 
                 33.33 
                 78.47 
               
               
                 Fetal Liver Clontech 
                 94.45949484 
                 93.8962252 
                 94.18 
                 10.40 
                 4.81 
                 452.78 
               
               
                 Lung 
                 13.84782444 
                 19.95367566 
                 16.90 
                 2.57 
                 19.46 
                 328.81 
               
               
                 Mammary Gland 
                 107.7956161 
                 95.02632495 
                 101.41 
                 13.00 
                 3.85 
                 390.04 
               
               
                 Clontech 
               
               
                 Myometrium 
                 12.50117866 
                 14.93742804 
                 13.72 
                 2.34 
                 21.37 
                 293.15 
               
               
                 Omentum 
                 13.998213 
                 22.03816357 
                 18.02 
                 3.94 
                 12.69 
                 228.66 
               
               
                 Ovary 
                 0.965703497 
                 0.965703497 
                 0.97 
                 4.34 
                 11.52 
                 11.13 
               
               
                 Pancreas 
                 2.254750425 
                 0.965703497 
                 1.61 
                 0.81 
                 61.80 
                 99.52 
               
               
                 Head of Pancreas 
                 0.965703497 
                 0.965703497 
                 0.97 
                 1.57 
                 31.85 
                 30.75 
               
               
                 Parotid Gland 
                 25.8930892 
                 14.85668173 
                 20.37 
                 5.48 
                 9.12 
                 185.90 
               
               
                 Placenta Clontech 
                 83.84029668 
                 95.02632495 
                 89.43 
                 5.26 
                 9.51 
                 850.13 
               
               
                 Prostate 
                 8.047386733 
                 15.18245262 
                 11.61 
                 3.00 
                 16.67 
                 193.58 
               
               
                 Rectum 
                 10.53572882 
                 20.06385011 
                 15.30 
                 1.23 
                 40.65 
                 621.94 
               
               
                 Salivary Gland 
                 62.43024331 
                 57.19623352 
                 59.81 
                 7.31 
                 6.84 
                 409.12 
               
               
                 Clontech 
               
               
                 Skeletal Muscle 
                 1.376746214 
                 0.965703497 
                 1.17 
                 1.26 
                 39.68 
                 46.48 
               
               
                 Clontech 
               
               
                 Skin 
                 0.965703497 
                 0.965703497 
                 0.97 
                 1.21 
                 41.32 
                 39.91 
               
               
                 Small Intestine 
                 0.965703497 
                 0.965703497 
                 0.97 
                 0.98 
                 51.07 
                 49.32 
               
               
                 Clontech 
               
               
                 Spleen 
                 0.965703497 
                 5.740147492 
                 3.35 
                 4.92 
                 10.16 
                 34.07 
               
               
                 Stomach 
                 0.965703497 
                 0.965703497 
                 0.97 
                 2.73 
                 18.32 
                 17.69 
               
               
                 Testis Clontech 
                 0.965703497 
                 0.965703497 
                 0.97 
                 0.57 
                 87.87 
                 84.86 
               
               
                 Thymus Clontech 
                 258.7386545 
                 207.7169358 
                 233.23 
                 9.89 
                 5.06 
                 1179.11 
               
               
                 Thyroid 
                 12.56849785 
                 19.09489343 
                 15.83 
                 2.77 
                 18.05 
                 285.77 
               
               
                 Trachea Clontech 
                 24.35330878 
                 31.87047641 
                 28.11 
                 9.71 
                 5.15 
                 144.76 
               
               
                 Urinary Bladder 
                 51.81831091 
                 57.53035871 
                 54.67 
                 5.47 
                 9.14 
                 499.77 
               
               
                 Uterus 
                 13.12099559 
                 14.61718971 
                 13.87 
                 5.34 
                 9.36 
                 129.86 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                   
                 copies of mRNA 
                   
                 Fold Change 
               
               
                 Sample 
                 (GSK 
                 Mean GOI 
                 detected/50 ng 
                   
                 in Disease 
               
               
                 sbg417005LAMININ 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 15446.92728 
                 30893.85 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 23910.90415 
                 47821.81 
                 colon tumor 
                 1.547939193 
               
               
                 colon normal GW98-178 
                 22080 
                 14621.97321 
                 29243.95 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 2058.30396 
                 4116.61 
                 colon tumor 
                 −7.10389403 
               
               
                 colon normal GW98-561 
                 23514 
                 5590.900474 
                 11181.80 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 12318.10362 
                 24636.21 
                 colon tumor 
                 2.203241442 
               
               
                 colon normal GW98-894 
                 24691 
                 4478.692403 
                 8957.38 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 7546.100944 
                 15092.20 
                 colon tumor 
                 1.684889308 
               
               
                 lung normal GW98-3 
                 20742 
                 23910.90415 
                 47821.81 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 35021.23317 
                 70042.47 
                 lung tumor 
                 1.464655328 
               
               
                 lung normal GW97-179 
                 20677 
                 23341.61421 
                 46683.23 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 24103.90252 
                 48207.81 
                 lung tumor 
                 1.032657909 
               
               
                 lung normal GW98-165 
                 21922 
                 18374.41273 
                 36748.83 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 34735.19726 
                 69470.39 
                 lung tumor 
                 1.890411289 
               
               
                 lung normal GW98-282 
                 22584 
                 3002.298467 
                 6004.60 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 3519.560955 
                 7039.12 
                 lung tumor 
                 1.172288829 
               
               
                 breast normal GW00-392 
                 28750 
                 5978.671937 
                 5978.67 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 5674.721186 
                 11349.44 
                 breast tumor 
                 1.898321649 
               
               
                 breast normal GW00-413 
                 28798 
                 1523.643258 
                 1523.64 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 956.0902914 
                 1912.18 
                 breast tumor 
                 1.255005444 
               
               
                 breast normal GW00- 
                 27592-95 
                 760.6128764 
                 760.61 
                 breast 
               
               
                 235:238 
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00- 
                 27588-91 
                 4192.50003 
                 4192.50 
                 breast tumor 
                 5.51200244 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 5674.721186 
                 11349.44 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 8017.202071 
                 16034.40 
                 breast tumor 
                 1.412792243 
               
               
                 brain normal BB99-542 
                 25507 
                 791.7818289 
                 1583.56 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 524.990001 
                 1049.98 
                 brain normal 
               
               
                 brain normal BB99-904 
                 25546 
                 396.8655236 
                 793.73 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 3203.498645 
                 6407.00 
                 brain stage 5 
                 5.608243725 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 3925.505917 
                 7851.01 
                 brain stage 5 
                 6.872234505 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 1502.651942 
                 3005.30 
                 brain stage 5 
                 2.630635833 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 1555.711325 
                 3111.42 
                 brain stage 5 
                 2.723524884 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 3730.249874 
                 7460.50 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 286.3143862 
                 286.31 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 72.30560941 
                 72.31 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 28.47771374 
                 28.48 
                 lung 24 
                 −69.25877363 
               
               
                 lung 28 KC 
                 COPD 
                 66.98006875 
                 66.98 
                 lung 28 
                 −29.44654382 
               
               
                 lung 23 KC 
                 COPD 
                 57.53035871 
                 57.53 
                 lung 23 
                 −34.28331708 
               
               
                 lung 25 KC 
                 COPD 
                 70.20637402 
                 70.21 
                 lung 25 
               
               
                 asthmatic lung 
                 29321 
                 2304.915385 
                 2304.92 
                 asthmatic 
                 1.168624722 
               
               
                 ODO3112 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29323 
                 3112.377018 
                 6224.75 
                 asthmatic 
                 3.156038395 
               
               
                 ODO3433 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29322 
                 21892.2071 
                 43784.41 
                 asthmatic 
                 22.19931768 
               
               
                 ODO3397 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29325 
                 5268.438364 
                 10536.88 
                 asthmatic 
                 5.34234563 
               
               
                 ODO4928 
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 396.8655236 
                 396.87 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 157.1987188 
                 157.20 
                 endo VEGF 
                 −2.524610421 
               
               
                 endo bFGF KC 
                   
                 518.1542863 
                 518.15 
                 endo bFGF 
                 1.305616778 
               
               
                 heart Clontech 
                 normal 
                 1865.302957 
                 3730.61 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 3757.505456 
                 7515.01 
                 heart T-1 
                 2.014421005 
               
               
                 heart (T-14) non- 
                 29422 
                 1633.333543 
                 3266.67 
                 heart T-14 
                 −1.142022072 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399) DCM 
                 29426 
                 2938.226492 
                 5876.45 
                 heart T-3399 
                 1.575200683 
               
               
                 adenoid GW99-269 
                 26162 
                 1238.725105 
                 2477.45 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 2288.625236 
                 4577.25 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 61.34444995 
                 122.69 
                 T cells 
               
               
                 PBMNC KC 
                   
                 5.341492957 
                 5.34 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 3.576686692 
                 7.15 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 716.2601536 
                 1432.52 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 32.23243314 
                 64.46 
                 dendritic 
               
               
                   
                   
                   
                   
                 cells 
               
               
                 neutrophils 
                 28440 
                 32.9693996 
                 32.97 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 1.444144312 
                 2.89 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 5.951115795 
                 5.95 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 11.72233235 
                 11.72 
                 BM stim 
                 1.969770503 
               
               
                 osteo dif KC 
                   
                 10.20495465 
                 10.20 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 8.526098078 
                 8.53 
                 osteo undif 
                 −1.196907959 
               
               
                 chondrocytes 
                   
                 14621.97321 
                 36554.93 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 5549.480142 
                 5549.48 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 3545.197127 
                 7090.39 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 4223.325454 
                 8446.65 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 1221.845309 
                 2443.69 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 4892.67872 
                 9785.36 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 1080.396739 
                 2160.79 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 995.7612933 
                 995.76 
                 OA bone 
               
               
                   
                   
                   
                   
                 (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 982.3483914 
                 1964.70 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 472.8535333 
                 945.71 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 1213.496434 
                 2426.99 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 697.4302173 
                 1394.86 
                 Cartilage 
                 −1.73995391 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 161.1142664 
                 322.23 
                 PBL 
               
               
                   
                   
                   
                   
                 unifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 191.5686557 
                 383.14 
                 PBL HIV 
                 1.189023542 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected 
                 29158 
                 5934.220593 
                 11868.44 
                 MRC5 
               
               
                 (100%) 
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 50.63206269 
                 101.26 
                 MRC5 HSV 
                 −117.2028213 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 13843.2955 
                 27686.59 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 11849.9156 
                 23699.83 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg417005LAMININ 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 1.55 
               
               
                   
                 colon tumor 
                 −7.10 
               
               
                   
                 colon tumor 
                 2.20 
               
               
                   
                 colon tumor 
                 1.68 
               
               
                   
                 lung tumor 
                 1.46 
               
               
                   
                 lung tumor 
                 1.03 
               
               
                   
                 lung tumor 
                 1.89 
               
               
                   
                 lung tumor 
                 1.17 
               
               
                   
                 breast tumor 
                 1.90 
               
               
                   
                 breast tumor 
                 1.26 
               
               
                   
                 breast tumor 
                 5.51 
               
               
                   
                 breast tumor 
                 1.41 
               
               
                   
                 brain stage 5 ALZ 
                 5.61 
               
               
                   
                 brain stage 5 ALZ 
                 6.87 
               
               
                   
                 brain stage 5 ALZ 
                 2.63 
               
               
                   
                 brain stage 5 ALZ 
                 2.72 
               
               
                   
                 lung 24 
                 −69.26 
               
               
                   
                 lung 28 
                 −29.45 
               
               
                   
                 lung 23 
                 −34.28 
               
               
                   
                 asthmatic lung 
                 1.17 
               
               
                   
                 asthmatic lung 
                 3.16 
               
               
                   
                 asthmatic lung 
                 22.20 
               
               
                   
                 asthmatic lung 
                 5.34 
               
               
                   
                 endo VEGF 
                 −2.52 
               
               
                   
                 endo bFGF 
                 1.31 
               
               
                   
                 heart T-1 
                 2.01 
               
               
                   
                 heart T-14 
                 −1.14 
               
               
                   
                 heart T-3399 
                 1.58 
               
               
                   
                 BM stim 
                 1.97 
               
               
                   
                 osteo undif 
                 −1.20 
               
               
                   
                 Cartilage (pool) 
                 −1.74 
               
               
                   
                 PBL HIV IIIB 
                 1.19 
               
               
                   
                 MRC5 HSV strain F 
                 −117.20 
               
               
                   
                   
               
            
           
           
               
            
               
                 Gene Name sbg425649KINASEa 
               
            
           
           
               
            
               
                 Strongly expressed in neutrophils and eosinophils suggesting function in immume system such as involvement in 
               
               
                 allergic reactions and anti-infective. Lower expression in T-cells. Expression in 2/3 OA bone samples indicate 
               
               
                 a role in OA. Strongly expressed in rectum and skeletal muscle, unknown function. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 sbg425649KINASEa 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 0.00 
                 0.03 
                 0.02 
                 3.06 
                 16.34 
                 0.25 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 0.00 
                 0.00 
                 0.00 
                 0.96 
                 52.36 
                 0.00 
               
               
                 Zenbio 
               
               
                 Adrenal Gland Clontech 
                 0.23 
                 0.00 
                 0.12 
                 0.61 
                 81.97 
                 9.43 
               
               
                 Whole Brain Clontech 
                 163.64 
                 47.63 
                 105.64 
                 7.24 
                 6.91 
                 729.52 
               
               
                 Fetal Brain Clontech 
                 0.47 
                 0.00 
                 0.24 
                 0.48 
                 103.95 
                 24.43 
               
               
                 Cerebellum Clontech 
                 0.00 
                 0.00 
                 0.00 
                 2.17 
                 23.04 
                 0.00 
               
               
                 Cervix 
                 5.54 
                 0.00 
                 2.77 
                 2.42 
                 20.66 
                 57.23 
               
               
                 Colon 
                 0.70 
                 0.00 
                 0.35 
                 2.71 
                 18.45 
                 6.46 
               
               
                 Endometrium 
                 0.33 
                 0.06 
                 0.20 
                 0.73 
                 68.21 
                 13.30 
               
               
                 Esophagus 
                 0.35 
                 0.47 
                 0.41 
                 1.37 
                 36.50 
                 14.96 
               
               
                 Heart Clontech 
                 0.00 
                 0.00 
                 0.00 
                 1.32 
                 37.88 
                 0.00 
               
               
                 Hypothalamus 
                 0.00 
                 0.00 
                 0.00 
                 0.32 
                 155.28 
                 0.00 
               
               
                 Ileum 
                 0.00 
                 4.49 
                 2.25 
                 2.58 
                 19.38 
                 43.51 
               
               
                 Jejunum 
                 0.29 
                 0.73 
                 0.51 
                 6.60 
                 7.58 
                 3.86 
               
               
                 Kidney 
                 0.00 
                 0.00 
                 0.00 
                 2.12 
                 23.58 
                 0.00 
               
               
                 Liver 
                 10.48 
                 5.64 
                 8.06 
                 1.50 
                 33.33 
                 268.67 
               
               
                 Fetal Liver Clontech 
                 8.56 
                 0.00 
                 4.28 
                 10.40 
                 4.81 
                 20.58 
               
               
                 Lung 
                 0.00 
                 0.00 
                 0.00 
                 2.57 
                 19.46 
                 0.00 
               
               
                 Mammary Gland 
                 0.00 
                 0.00 
                 0.00 
                 13.00 
                 3.85 
                 0.00 
               
               
                 Clontech 
               
               
                 Myometrium 
                 8.61 
                 5.00 
                 6.81 
                 2.34 
                 21.37 
                 145.41 
               
               
                 Omentum 
                 0.23 
                 10.99 
                 5.61 
                 3.94 
                 12.69 
                 71.19 
               
               
                 Ovary 
                 4.48 
                 4.62 
                 4.55 
                 4.34 
                 11.52 
                 52.42 
               
               
                 Pancreas 
                 0.27 
                 0.00 
                 0.14 
                 0.81 
                 61.80 
                 8.34 
               
               
                 Head of Pancreas 
                 0.11 
                 0.04 
                 0.08 
                 1.57 
                 31.85 
                 2.39 
               
               
                 Parotid Gland 
                 0.69 
                 4.51 
                 2.60 
                 5.48 
                 9.12 
                 23.72 
               
               
                 Placenta Clontech 
                 10.58 
                 0.14 
                 5.36 
                 5.26 
                 9.51 
                 50.95 
               
               
                 Prostate 
                 9.74 
                 6.18 
                 7.96 
                 3.00 
                 16.67 
                 132.67 
               
               
                 Rectum 
                 225.51 
                 76.99 
                 151.25 
                 1.23 
                 40.65 
                 6148.37 
               
               
                 Salivary Gland 
                 60.93 
                 67.22 
                 64.08 
                 7.31 
                 6.84 
                 438.27 
               
               
                 Clontech 
               
               
                 Skeletal Muscle 
                 749.28 
                 29.78 
                 389.53 
                 1.26 
                 39.68 
                 15457.54 
               
               
                 Clontech 
               
               
                 Skin 
                 0.00 
                 4.46 
                 2.23 
                 1.21 
                 41.32 
                 92.15 
               
               
                 Small Intestine 
                 0.73 
                 0.00 
                 0.37 
                 0.98 
                 51.07 
                 18.64 
               
               
                 Clontech 
               
               
                 Spleen 
                 4.10 
                 8.60 
                 6.35 
                 4.92 
                 10.16 
                 64.53 
               
               
                 Stomach 
                 4.24 
                 19.28 
                 11.76 
                 2.73 
                 18.32 
                 215.38 
               
               
                 Testis Clontech 
                 10.11 
                 6.34 
                 8.23 
                 0.57 
                 87.87 
                 722.76 
               
               
                 Thymus Clontech 
                 2.79 
                 5.35 
                 4.07 
                 9.89 
                 5.06 
                 20.58 
               
               
                 Thyroid 
                 0.00 
                 0.06 
                 0.03 
                 2.77 
                 18.05 
                 0.54 
               
               
                 Trachea Clontech 
                 5.24 
                 14.14 
                 9.69 
                 9.71 
                 5.15 
                 49.90 
               
               
                 Urinary Bladder 
                 0.09 
                 0.00 
                 0.05 
                 5.47 
                 9.14 
                 0.41 
               
               
                 Uterus 
                 27.26 
                 7.61 
                 17.44 
                 5.34 
                 9.36 
                 163.25 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                 Mean 
                 copies of mRNA 
                   
                 Fold Change 
               
               
                 Sample 
                 (GSK 
                 GOI 
                 detected/50 ng 
                   
                 in Disease 
               
               
                 sbg425649KINASEa 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 11.11 
                 22.22 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 7.3 
                 14.60 
                 colon tumor 
                 −1.521917808 
               
               
                 colon normal GW98-178 
                 22080 
                 0 
                 0.00 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 2.57 
                 5.14 
                 colon tumor 
                 5.14 
               
               
                 colon normal GW98-561 
                 23514 
                 0 
                 0.00 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 0 
                 0.00 
                 colon tumor 
                 0 
               
               
                 colon normal GW98-894 
                 24691 
                 2.71 
                 5.42 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 8.51 
                 17.02 
                 colon tumor 
                 3.140221402 
               
               
                 lung normal GW98-3 
                 20742 
                 1.78 
                 3.56 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 0 
                 0.00 
                 lung tumor 
                 −3.56 
               
               
                 lung normal GW97-179 
                 20677 
                 3.18 
                 6.36 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 2.64 
                 5.28 
                 lung tumor 
                 −1.204545455 
               
               
                 lung normal GW98-165 
                 21922 
                 6.46 
                 12.92 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 19.99 
                 39.98 
                 lung tumor 
                 3.094427245 
               
               
                 lung normal GW98-282 
                 22584 
                 31.56 
                 63.12 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 7.47 
                 14.94 
                 lung tumor 
                 −4.224899598 
               
               
                 breast normal GW00-392 
                 28750 
                 5.68 
                 5.68 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 2.87 
                 5.74 
                 breast tumor 
                 1.01056338 
               
               
                 breast normal GW00-413 
                 28798 
                 1.66 
                 1.66 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 1.99 
                 3.98 
                 breast tumor 
                 2.397590361 
               
               
                 breast normal GW00- 
                 27592-95 
                 0 
                 0.00 
                 breast 
               
               
                 235:238 
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00- 
                 27588-91 
                 2.19 
                 2.19 
                 breast tumor 
                 2.19 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 4.72 
                 9.44 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 0 
                 0.00 
                 breast tumor 
                 −9.44 
               
               
                 brain normal BB99-542 
                 25507 
                 28.9 
                 57.80 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 24.84 
                 49.68 
                 brain normal 
               
               
                 brain normal BB99-904 
                 25546 
                 6.92 
                 13.84 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 23.65 
                 47.30 
                 brain stage 5 
                 1.169634026 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 28.68 
                 57.36 
                 brain stage 5 
                 1.418397626 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 18.18 
                 36.36 
                 brain stage 5 
                 −1.112211221 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 14.18 
                 28.36 
                 brain stage 5 
                 −1.425952045 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 29.45 
                 58.90 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 2.47 
                 2.47 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 0 
                 0.00 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 0 
                 0.00 
                 lung 24 
                 −15.3425 
               
               
                 lung 28 KC 
                 COPD 
                 0.3 
                 0.30 
                 lung 28 
                 −51.14166667 
               
               
                 lung 23 KC 
                 COPD 
                 0 
                 0.00 
                 lung 23 
                 −15.3425 
               
               
                 lung 25 KC 
                 COPD 
                 0 
                 0.00 
                 lung 25 
               
               
                 asthmatic lung 
                 29321 
                 3.24 
                 3.24 
                 asthmatic 
                 −4.735339506 
               
               
                 ODO3112 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29323 
                 88.32 
                 176.64 
                 asthmatic 
                 11.51311716 
               
               
                 ODO3433 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29322 
                 55.65 
                 111.30 
                 asthmatic 
                 7.254358807 
               
               
                 ODO3397 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29325 
                 50.64 
                 101.28 
                 asthmatic 
                 6.601270979 
               
               
                 ODO4928 
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 0 
                 0.00 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 0 
                 0.00 
                 endo VEGF 
                 0 
               
               
                 endo bFGF KC 
                   
                 0 
                 0.00 
                 endo bFGF 
                 0 
               
               
                 heart Clontech 
                 normal 
                 15.26 
                 30.52 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 0 
                 0.00 
                 heart T-1 
                 −30.52 
               
               
                 heart (T-14) non- 
                 29422 
                 3.69 
                 7.38 
                 heart T-14 
                 −4.135501355 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399) DCM 
                 29426 
                 0 
                 0.00 
                 heart T-3399 
                 −30.52 
               
               
                 adenoid GW99-269 
                 26162 
                 0 
                 0.00 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 3.65 
                 7.30 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 167.51 
                 335.02 
                 T cells 
               
               
                 PBMNC KC 
                   
                 2.5 
                 2.50 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 2.37 
                 4.74 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 0 
                 0.00 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 0 
                 0.00 
                 dendritic 
               
               
                   
                   
                   
                   
                 cells 
               
               
                 neutrophils 
                 28440 
                 1576.76 
                 1576.76 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 755.1 
                 1510.20 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 14.87 
                 14.87 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 45.45 
                 45.45 
                 BM stim 
                 3.056489576 
               
               
                 osteo dif KC 
                   
                 0 
                 0.00 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 0 
                 0.00 
                 osteo undif 
                 0 
               
               
                 chondrocytes 
                   
                 7.48 
                 18.70 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 17.79 
                 17.79 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 14.09 
                 28.18 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 11.97 
                 23.94 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 6.84 
                 13.68 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 22.88 
                 45.76 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 1.64 
                 3.28 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 370.22 
                 370.22 
                 OA bone 
               
               
                   
                   
                   
                   
                 (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 3.21 
                 6.42 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 311.65 
                 623.30 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 32.23 
                 64.46 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 2.87 
                 5.74 
                 Cartilage 
                 −11.22996516 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 4.18 
                 8.36 
                 PBL 
               
               
                   
                   
                   
                   
                 unifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 0 
                 0.00 
                 PBL HIV 
                 −8.36 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected 
                 29158 
                 4.4 
                 8.80 
                 MRC5 
               
               
                 (100%) 
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 11.46 
                 22.92 
                 MRC5 HSV 
                 2.604545455 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 0 
                 0.00 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 0 
                 0.00 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg425649KINASEa 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 −1.52 
               
               
                   
                 colon tumor 
                 5.14 
               
               
                   
                 colon tumor 
                 0.00 
               
               
                   
                 colon tumor 
                 3.14 
               
               
                   
                 lung tumor 
                 −3.56 
               
               
                   
                 lung tumor 
                 −1.20 
               
               
                   
                 lung tumor 
                 3.09 
               
               
                   
                 lung tumor 
                 −4.22 
               
               
                   
                 breast tumor 
                 1.01 
               
               
                   
                 breast tumor 
                 2.40 
               
               
                   
                 breast tumor 
                 2.19 
               
               
                   
                 breast tumor 
                 −9.44 
               
               
                   
                 brain stage 5 ALZ 
                 1.17 
               
               
                   
                 brain stage 5 ALZ 
                 1.42 
               
               
                   
                 brain stage 5 ALZ 
                 −1.11 
               
               
                   
                 brain stage 5 ALZ 
                 −1.43 
               
               
                   
                 lung 24 
                 −15.34 
               
               
                   
                 lung 28 
                 −51.14 
               
               
                   
                 lung 23 
                 −15.34 
               
               
                   
                 asthmatic lung 
                 −4.74 
               
               
                   
                 asthmatic lung 
                 11.51 
               
               
                   
                 asthmatic lung 
                 7.25 
               
               
                   
                 asthmatic lung 
                 6.60 
               
               
                   
                 endo VEGF 
                 0.00 
               
               
                   
                 endo bFGF 
                 0.00 
               
               
                   
                 heart T-1 
                 −30.52 
               
               
                   
                 heart T-14 
                 −4.14 
               
               
                   
                 heart T-3399 
                 −30.52 
               
               
                   
                 BM stim 
                 3.06 
               
               
                   
                 osteo undif 
                 0.00 
               
               
                   
                 Cartilage (pool) 
                 −11.23 
               
               
                   
                 PBL HIV IIIB 
                 −8.36 
               
               
                   
                 MRC5 HSV strain F 
                 2.60 
               
               
                   
                   
               
            
           
           
               
            
               
                 Gene Name sbg419582PROTOCADHERIN 
               
            
           
           
               
            
               
                 Brain specific expression. No correlation with Alzheimer&#39;s disease. Low expression in RA and OA synovium but 
               
               
                 no corroborating expression in immune cells. Slightly upregulated in heart disease. Overexpressed in lung (1/4) 
               
               
                 and breast (1/4) tumors. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 sbg419582PROTOCADHERIN 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 18.18 
                 23.43 
                 20.81 
                 3.06 
                 16.34 
                 339.95 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 0.11 
                 0.33 
                 0.22 
                 0.96 
                 52.36 
                 11.52 
               
               
                 Zenbio 
               
               
                 Adrenal Gland Clontech 
                 1.8 
                 1.06 
                 1.43 
                 0.61 
                 81.97 
                 117.21 
               
               
                 Whole Brain Clontech 
                 10913.92 
                 10314.42 
                 10614.17 
                 7.24 
                 6.91 
                 73302.28 
               
               
                 Fetal Brain Clontech 
                 0.31 
                 4.68 
                 2.50 
                 0.48 
                 103.95 
                 259.36 
               
               
                 Cerebellum Clontech 
                 0.1 
                 4.58 
                 2.34 
                 2.17 
                 23.04 
                 53.92 
               
               
                 Cervix 
                 0.22 
                 1.22 
                 0.72 
                 2.42 
                 20.66 
                 14.88 
               
               
                 Colon 
                 0.31 
                 13.73 
                 7.02 
                 2.71 
                 18.45 
                 129.52 
               
               
                 Endometrium 
                 0.1 
                 0.58 
                 0.34 
                 0.73 
                 68.21 
                 23.19 
               
               
                 Esophagus 
                 2.21 
                 1.96 
                 2.09 
                 1.37 
                 36.50 
                 76.09 
               
               
                 Heart Clontech 
                 0.32 
                 0 
                 0.16 
                 1.32 
                 37.88 
                 6.06 
               
               
                 Hypothalamus 
                 0.15 
                 1.2 
                 0.68 
                 0.32 
                 155.28 
                 104.81 
               
               
                 Ileum 
                 2.77 
                 1.03 
                 1.90 
                 2.58 
                 19.38 
                 36.82 
               
               
                 Jejunum 
                 0.26 
                 1.18 
                 0.72 
                 6.60 
                 7.58 
                 5.45 
               
               
                 Kidney 
                 1.99 
                 0.28 
                 1.14 
                 2.12 
                 23.58 
                 26.77 
               
               
                 Liver 
                 7.59 
                 12.42 
                 10.01 
                 1.50 
                 33.33 
                 333.50 
               
               
                 Fetal Liver Clontech 
                 18.75 
                 11.04 
                 14.90 
                 10.40 
                 4.81 
                 71.61 
               
               
                 Lung 
                 7.19 
                 0.71 
                 3.95 
                 2.57 
                 19.46 
                 76.85 
               
               
                 Mammary Gland 
                 88.14 
                 97.88 
                 93.01 
                 13.00 
                 3.85 
                 357.73 
               
               
                 Clontech 
               
               
                 Myometrium 
                 0.51 
                 4.8 
                 2.66 
                 2.34 
                 21.37 
                 56.73 
               
               
                 Omentum 
                 7.52 
                 2.19 
                 4.86 
                 3.94 
                 12.69 
                 61.61 
               
               
                 Ovary 
                 13.46 
                 4.84 
                 9.15 
                 4.34 
                 11.52 
                 105.41 
               
               
                 Pancreas 
                 0.49 
                 1.02 
                 0.76 
                 0.81 
                 61.80 
                 46.66 
               
               
                 Head of Pancreas 
                 0.29 
                 0.15 
                 0.22 
                 1.57 
                 31.85 
                 7.01 
               
               
                 Parotid Gland 
                 6.09 
                 6.19 
                 6.14 
                 5.48 
                 9.12 
                 56.02 
               
               
                 Placenta Clontech 
                 10.67 
                 2.35 
                 6.51 
                 5.26 
                 9.51 
                 61.88 
               
               
                 Prostate 
                 2.02 
                 3.59 
                 2.81 
                 3.00 
                 16.67 
                 46.75 
               
               
                 Rectum 
                 0.54 
                 7.25 
                 3.90 
                 1.23 
                 40.65 
                 158.33 
               
               
                 Salivary Gland 
                 20.51 
                 13.73 
                 17.12 
                 7.31 
                 6.84 
                 117.10 
               
               
                 Clontech 
               
               
                 Skeletal Muscle 
                 1.06 
                 0.79 
                 0.93 
                 1.26 
                 39.68 
                 36.71 
               
               
                 Clontech 
               
               
                 Skin 
                 13.09 
                 0.6 
                 6.85 
                 1.21 
                 41.32 
                 282.85 
               
               
                 Small Intestine 
                 0.11 
                 2.47 
                 1.29 
                 0.98 
                 51.07 
                 65.88 
               
               
                 Clontech 
               
               
                 Spleen 
                 1.05 
                 11 
                 6.03 
                 4.92 
                 10.16 
                 61.23 
               
               
                 Stomach 
                 0.95 
                 1.3 
                 1.13 
                 2.73 
                 18.32 
                 20.60 
               
               
                 Testis Clontech 
                 2.82 
                 3.19 
                 3.01 
                 0.57 
                 87.87 
                 264.06 
               
               
                 Thymus Clontech 
                 117.82 
                 118.81 
                 118.32 
                 9.89 
                 5.06 
                 598.15 
               
               
                 Thyroid 
                 2.34 
                 2.29 
                 2.32 
                 2.77 
                 18.05 
                 41.79 
               
               
                 Trachea Clontech 
                 8.72 
                 9.37 
                 9.05 
                 9.71 
                 5.15 
                 46.58 
               
               
                 Urinary Bladder 
                 14.23 
                 16.82 
                 15.53 
                 5.47 
                 9.14 
                 141.91 
               
               
                 Uterus 
                 1.49 
                 27.26 
                 14.38 
                 5.34 
                 9.36 
                 134.60 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                 Mean 
                 copies of mRNA 
                   
                 Fold Change 
               
               
                 Sample 
                 (GSK 
                 GOI 
                 detected/50 ng 
                   
                 in Disease 
               
               
                 sbg419582PROTOCADHERIN 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 464.48 
                 928.96 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 84.22 
                 168.44 
                 colon tumor 
                 −5.515079554 
               
               
                 colon normal GW98-178 
                 22080 
                 32.8 
                 65.60 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 44.71 
                 89.42 
                 colon tumor 
                 1.363109756 
               
               
                 colon normal GW98-561 
                 23514 
                 135.5 
                 271.00 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 78.51 
                 157.02 
                 colon tumor 
                 −1.72589479 
               
               
                 colon normal GW98-894 
                 24691 
                 454.16 
                 908.32 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 51.37 
                 102.74 
                 colon tumor 
                 −8.840957757 
               
               
                 lung normal GW98-3 
                 20742 
                 60.35 
                 120.70 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 101.98 
                 203.96 
                 lung tumor 
                 1.689809445 
               
               
                 lung normal GW97-179 
                 20677 
                 264 
                 528.00 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 78.49 
                 156.98 
                 lung tumor 
                 −3.363485794 
               
               
                 lung normal GW98-165 
                 21922 
                 88.19 
                 176.38 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 7554.58 
                 15109.16 
                 lung tumor 
                 85.66254677 
               
               
                 lung normal GW98-282 
                 22584 
                 344.2 
                 688.40 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 45.51 
                 91.02 
                 lung tumor 
                 −7.563172929 
               
               
                 breast normal GW00-392 
                 28750 
                 132.43 
                 132.43 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 98.14 
                 196.28 
                 breast tumor 
                 1.482141509 
               
               
                 breast normal GW00-413 
                 28798 
                 154.37 
                 154.37 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 1289.09 
                 2578.18 
                 breast tumor 
                 16.70130207 
               
               
                 breast normal GW00- 
                 27592-95 
                 18.63 
                 18.63 
                 breast 
               
               
                 235:238 
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00- 
                 27588-91 
                 133.52 
                 133.52 
                 breast tumor 
                 7.166935051 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 1334.91 
                 2669.82 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 212.39 
                 424.78 
                 breast tumor 
                 −6.285182918 
               
               
                 brain normal BB99-542 
                 25507 
                 6816.47 
                 13632.94 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 1984.48 
                 3968.96 
                 brain normal 
               
               
                 brain normal BB99-904 
                 25546 
                 2805.82 
                 5611.64 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 467.59 
                 935.18 
                 brain stage 5 
                 −8.274178946 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 3104.22 
                 6208.44 
                 brain stage 5 
                 −1.24634315 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 1889.81 
                 3779.62 
                 brain stage 5 
                 −2.047255191 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 2902.29 
                 5804.58 
                 brain stage 5 
                 −1.333058837 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 103.32 
                 206.64 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 1.13 
                 1.13 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 1.51 
                 1.51 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 1.47 
                 1.47 
                 lung 24 
                 −35.82312925 
               
               
                 lung 28 KC 
                 COPD 
                 0 
                 0.00 
                 lung 28 
                 −52.66 
               
               
                 lung 23 KC 
                 COPD 
                 1.91 
                 1.91 
                 lung 23 
                 −27.57068063 
               
               
                 lung 25 KC 
                 COPD 
                 1.36 
                 1.36 
                 lung 25 
               
               
                 asthmatic lung 
                 29321 
                 2.68 
                 2.68 
                 asthmatic 
                 −19.64925373 
               
               
                 ODO3112 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29323 
                 3.25 
                 6.50 
                 asthmatic 
                 −8.101538462 
               
               
                 ODO3433 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29322 
                 26.23 
                 52.46 
                 asthmatic 
                 −1.003812429 
               
               
                 ODO3397 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29325 
                 7.15 
                 14.30 
                 asthmatic 
                 −3.682517483 
               
               
                 ODO4928 
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 15.9 
                 15.90 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 8.26 
                 8.26 
                 endo VEGF 
                 −1.924939467 
               
               
                 endo bFGF KC 
                   
                 2.01 
                 2.01 
                 endo bFGF 
                 −7.910447761 
               
               
                 heart Clontech 
                 normal 
                 7.9 
                 15.80 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 67.47 
                 134.94 
                 heart T-1 
                 8.540506329 
               
               
                 heart (T-14) non- 
                 29422 
                 106.83 
                 213.66 
                 heart T-14 
                 13.52278481 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399) DCM 
                 29426 
                 425.28 
                 850.56 
                 heart T-3399 
                 53.83291139 
               
               
                 adenoid GW99-269 
                 26162 
                 15.98 
                 31.96 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 17.95 
                 35.90 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 3.18 
                 6.36 
                 T cells 
               
               
                 PBMNC KC 
                   
                 0 
                 0.00 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 0.81 
                 1.62 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 2.74 
                 5.48 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 0 
                 0.00 
                 dendritic 
               
               
                   
                   
                   
                   
                 cells 
               
               
                 neutrophils 
                 28440 
                 0 
                 0.00 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 0 
                 0.00 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 0 
                 0.00 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 0 
                 0.00 
                 BM stim 
                 0 
               
               
                 osteo dif KC 
                   
                 2.34 
                 2.34 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 0 
                 0.00 
                 osteo undif 
                 −2.34 
               
               
                 chondrocytes 
                   
                 145.14 
                 362.85 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 320.78 
                 320.78 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 396.85 
                 793.70 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 329.87 
                 659.74 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 103.85 
                 207.70 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 617.72 
                 1235.44 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 63.13 
                 126.26 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 3.19 
                 3.19 
                 OA bone 
               
               
                   
                   
                   
                   
                 (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 126.87 
                 253.74 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 44.76 
                 89.52 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 502.66 
                 1005.32 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 206.76 
                 413.52 
                 Cartilage 
                 −2.431127878 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 0 
                 0.00 
                 PBLunifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 0 
                 0.00 
                 PBL HIV 
                 0 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected 
                 29158 
                 0 
                 0.00 
                 MRC5 
               
               
                 (100%) 
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 17.73 
                 35.46 
                 MRC5 HSV 
                 35.46 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 0.62 
                 1.24 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 22.63 
                 45.26 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg419582PROTOCADHERIN 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 −5.52 
               
               
                   
                 colon tumor 
                 1.36 
               
               
                   
                 colon tumor 
                 −1.73 
               
               
                   
                 colon tumor 
                 −8.84 
               
               
                   
                 lung tumor 
                 1.69 
               
               
                   
                 lung tumor 
                 −3.36 
               
               
                   
                 lung tumor 
                 85.66 
               
               
                   
                 lung tumor 
                 −7.56 
               
               
                   
                 breast tumor 
                 1.48 
               
               
                   
                 breast tumor 
                 16.70 
               
               
                   
                 breast tumor 
                 7.17 
               
               
                   
                 breast tumor 
                 −6.29 
               
               
                   
                 brain stage 5 ALZ 
                 −8.27 
               
               
                   
                 brain stage 5 ALZ 
                 −1.25 
               
               
                   
                 brain stage 5 ALZ 
                 −2.05 
               
               
                   
                 brain stage 5 ALZ 
                 −1.33 
               
               
                   
                 lung 24 
                 −35.82 
               
               
                   
                 lung 28 
                 −52.66 
               
               
                   
                 lung 23 
                 −27.57 
               
               
                   
                 asthmatic lung 
                 −19.65 
               
               
                   
                 asthmatic lung 
                 −8.10 
               
               
                   
                 asthmatic lung 
                 −1.00 
               
               
                   
                 asthmatic lung 
                 −3.68 
               
               
                   
                 endo VEGF 
                 −1.92 
               
               
                   
                 endo bFGF 
                 −7.91 
               
               
                   
                 heart T-1 
                 8.54 
               
               
                   
                 heart T-14 
                 13.52 
               
               
                   
                 heart T-3399 
                 53.83 
               
               
                   
                 BM stim 
                 0.00 
               
               
                   
                 osteo undif 
                 −2.34 
               
               
                   
                 Cartilage (pool) 
                 −2.43 
               
               
                   
                 PBL HIV IIIB 
                 0.00 
               
               
                   
                 MRC5 HSV strain F 
                 35.46 
               
               
                   
                   
               
            
           
           
               
            
               
                 Gene Name sbg453915TECTORINa 
               
            
           
           
               
            
               
                 Very low expression overall. Expression in female reproductive tissues suggests a protein that may be secreted 
               
               
                 by these tissue types. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 sbg453915TECTORINa 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 2.70 
                 5.41 
                 4.06 
                 3.06 
                 16.34 
                 66.26 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 0.00 
                 0.00 
                 0.00 
                 0.96 
                 52.36 
                 0.00 
               
               
                 Zenbio 
               
               
                 Adrenal Gland Clontech 
                 3.75 
                 5.67 
                 4.71 
                 0.61 
                 81.97 
                 386.07 
               
               
                 Whole Brain Clontech 
                 22.57 
                 27.88 
                 25.23 
                 7.24 
                 6.91 
                 174.21 
               
               
                 Fetal Brain Clontech 
                 2.42 
                 1.80 
                 2.11 
                 0.48 
                 103.95 
                 219.33 
               
               
                 Cerebellum Clontech 
                 0.00 
                 1.93 
                 0.97 
                 2.17 
                 23.04 
                 22.24 
               
               
                 Cervix 
                 2.90 
                 2.10 
                 2.50 
                 2.42 
                 20.66 
                 51.65 
               
               
                 Colon 
                 11.19 
                 2.68 
                 6.94 
                 2.71 
                 18.45 
                 127.95 
               
               
                 Endometrium 
                 4.79 
                 19.31 
                 12.05 
                 0.73 
                 68.21 
                 821.96 
               
               
                 Esophagus 
                 2.06 
                 2.93 
                 2.50 
                 1.37 
                 36.50 
                 91.06 
               
               
                 Heart Clontech 
                 5.42 
                 7.31 
                 6.37 
                 1.32 
                 37.88 
                 241.10 
               
               
                 Hypothalamus 
                 0.00 
                 3.70 
                 1.85 
                 0.32 
                 155.28 
                 287.27 
               
               
                 Ileum 
                 3.72 
                 18.75 
                 11.24 
                 2.58 
                 19.38 
                 217.73 
               
               
                 Jejunum 
                 28.49 
                 49.80 
                 39.15 
                 6.60 
                 7.58 
                 296.55 
               
               
                 Kidney 
                 2.12 
                 4.37 
                 3.25 
                 2.12 
                 23.58 
                 76.53 
               
               
                 Liver 
                 15.74 
                 39.80 
                 27.77 
                 1.50 
                 33.33 
                 925.67 
               
               
                 Fetal Liver Clontech 
                 27.96 
                 26.14 
                 27.05 
                 10.40 
                 4.81 
                 130.05 
               
               
                 Lung 
                 0.00 
                 2.37 
                 1.19 
                 2.57 
                 19.46 
                 23.05 
               
               
                 Mammary Gland 
                 19.68 
                 19.22 
                 19.45 
                 13.00 
                 3.85 
                 74.81 
               
               
                 Clontech 
               
               
                 Myometrium 
                 3.40 
                 1.71 
                 2.56 
                 2.34 
                 21.37 
                 54.59 
               
               
                 Omentum 
                 14.33 
                 138.99 
                 76.66 
                 3.94 
                 12.69 
                 972.84 
               
               
                 Ovary 
                 46.55 
                 37.80 
                 42.18 
                 4.34 
                 11.52 
                 485.89 
               
               
                 Pancreas 
                 4.26 
                 2.19 
                 3.23 
                 0.81 
                 61.80 
                 199.32 
               
               
                 Head of Pancreas 
                 1.93 
                 1.52 
                 1.73 
                 1.57 
                 31.85 
                 54.94 
               
               
                 Parotid Gland 
                 4.04 
                 5.93 
                 4.99 
                 5.48 
                 9.12 
                 45.48 
               
               
                 Placenta Clontech 
                 3.69 
                 15.48 
                 9.59 
                 5.26 
                 9.51 
                 91.11 
               
               
                 Prostate 
                 7.94 
                 28.75 
                 18.35 
                 3.00 
                 16.67 
                 305.75 
               
               
                 Rectum 
                 11.09 
                 3.41 
                 7.25 
                 1.23 
                 40.65 
                 294.72 
               
               
                 Salivary Gland 
                 0.00 
                 1.45 
                 0.73 
                 7.31 
                 6.84 
                 4.96 
               
               
                 Clontech 
               
               
                 Skeletal Muscle 
                 4.76 
                 0.00 
                 2.38 
                 1.26 
                 39.68 
                 94.44 
               
               
                 Clontech 
               
               
                 Skin 
                 0.00 
                 1.39 
                 0.70 
                 1.21 
                 41.32 
                 28.72 
               
               
                 Small Intestine 
                 2.20 
                 1.41 
                 1.81 
                 0.98 
                 51.07 
                 92.19 
               
               
                 Clontech 
               
               
                 Spleen 
                 7.15 
                 8.12 
                 7.64 
                 4.92 
                 10.16 
                 77.59 
               
               
                 Stomach 
                 1.98 
                 0.00 
                 0.99 
                 2.73 
                 18.32 
                 18.13 
               
               
                 Testis Clontech 
                 6.83 
                 2.61 
                 4.72 
                 0.57 
                 87.87 
                 414.76 
               
               
                 Thymus Clontech 
                 0.00 
                 0.00 
                 0.00 
                 9.89 
                 5.06 
                 0.00 
               
               
                 Thyroid 
                 2.38 
                 1.88 
                 2.13 
                 2.77 
                 18.05 
                 38.45 
               
               
                 Trachea Clontech 
                 1.71 
                 9.25 
                 5.48 
                 9.71 
                 5.15 
                 28.22 
               
               
                 Urinary Bladder 
                 3.72 
                 8.22 
                 5.97 
                 5.47 
                 9.14 
                 54.57 
               
               
                 Uterus 
                 74.31 
                 73.54 
                 73.93 
                 5.34 
                 9.36 
                 692.18 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                 Mean 
                 copies of mRNA 
                   
                 Fold Change in 
               
               
                 Sample 
                 (GSK 
                 GOI 
                 detected/50 ng 
                   
                 Disease 
               
               
                 sbg453915TECTORINa 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 131.15 
                 262.30 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 85.76 
                 171.52 
                 colon tumor 
                 −1.529267724 
               
               
                 colon normal GW98-178 
                 22080 
                 1.82 
                 3.64 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 10.14 
                 20.28 
                 colon tumor 
                 5.571428571 
               
               
                 colon normal GW98-561 
                 23514 
                 14.25 
                 28.50 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 9.89 
                 19.78 
                 colon tumor 
                 −1.440849343 
               
               
                 colon normal GW98-894 
                 24691 
                 32.05 
                 64.10 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 53.06 
                 106.12 
                 colon tumor 
                 1.655538222 
               
               
                 lung normal GW98-3 
                 20742 
                 6.9 
                 13.80 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 0.81 
                 1.62 
                 lung tumor 
                 −8.518518519 
               
               
                 lung normal GW97-179 
                 20677 
                 1.19 
                 2.38 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 0 
                 0.00 
                 lung tumor 
                 −2.38 
               
               
                 lung normal GW98-165 
                 21922 
                 0.91 
                 1.82 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 5.99 
                 11.98 
                 lung tumor 
                 6.582417582 
               
               
                 lung normal GW98-282 
                 22584 
                 5.93 
                 11.86 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 1.54 
                 3.08 
                 lung tumor 
                 −3.850649351 
               
               
                 breast normal GW00-392 
                 28750 
                 6.88 
                 6.88 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 4.24 
                 8.48 
                 breast tumor 
                 1.23255814 
               
               
                 breast normal GW00-413 
                 28798 
                 0 
                 0.00 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 13.96 
                 27.92 
                 breast tumor 
                 27.92 
               
               
                 breast normal GW00- 
                 27592-95 
                 14.42 
                 14.42 
                 breast 
               
               
                 235:238 
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00- 
                 27588-91 
                 0 
                 0.00 
                 breast tumor 
                 −14.42 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 5.81 
                 11.62 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 0 
                 0.00 
                 breast tumor 
                 −11.62 
               
               
                 brain normal BB99-542 
                 25507 
                 20.59 
                 41.18 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 15.98 
                 31.96 
                 brain normal 
               
               
                 brain normal BB99-904 
                 25546 
                 2.38 
                 4.76 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 25.45 
                 50.90 
                 brain stage 5 
                 1.960205392 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 35.78 
                 71.56 
                 brain stage 5 
                 2.755840822 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 13.83 
                 27.66 
                 brain stage 5 
                 1.06521181 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 21.67 
                 43.34 
                 brain stage 5 
                 1.669062901 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 6.52 
                 13.04 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 2.1 
                 2.10 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 0.84 
                 0.84 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 1.25 
                 1.25 
                 lung 24 
                 −3.432 
               
               
                 lung 28 KC 
                 COPD 
                 0 
                 0.00 
                 lung 28 
                 −4.29 
               
               
                 lung 23 KC 
                 COPD 
                 1.16 
                 1.16 
                 lung 23 
                 −3.698275862 
               
               
                 lung 25 KC 
                 COPD 
                 1.18 
                 1.18 
                 lung 25 
               
               
                 asthmatic lung ODO3112 
                 29321 
                 4.9 
                 4.90 
                 asthmatic 
                 1.142191142 
               
               
                   
                   
                   
                   
                 lung 
               
               
                 asthmatic lung ODO3433 
                 29323 
                 0.83 
                 1.66 
                 asthmatic 
                 −2.584337349 
               
               
                   
                   
                   
                   
                 lung 
               
               
                 asthmatic lung ODO3397 
                 29322 
                 2.46 
                 4.92 
                 asthmatic 
                 1.146853147 
               
               
                   
                   
                   
                   
                 lung 
               
               
                 asthmatic lung ODO4928 
                 29325 
                 6 
                 12.00 
                 asthmatic 
                 2.797202797 
               
               
                   
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 2.52 
                 2.52 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 1.28 
                 1.28 
                 endo VEGF 
                 −1.96875 
               
               
                 endo bFGF KC 
                   
                 0 
                 0.00 
                 endo bFGF 
                 −2.52 
               
               
                 heart Clontech 
                 normal 
                 0 
                 0.00 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 3.58 
                 7.16 
                 heart T-1 
                 7.16 
               
               
                 heart (T-14) non- 
                 29422 
                 0 
                 0.00 
                 heart T-14 
                 0 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399)DCM 
                 29426 
                 0 
                 0.00 
                 heart T-3399 
                 0 
               
               
                 adenoid GW99-269 
                 26162 
                 2.29 
                 4.58 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 1.85 
                 3.70 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 4.29 
                 8.58 
                 T cells 
               
               
                 PBMNC KC 
                   
                 0 
                 0.00 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 3.39 
                 6.78 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 6.04 
                 12.08 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 0.83 
                 1.66 
                 dendritic 
               
               
                   
                   
                   
                   
                 cells 
               
               
                 neutrophils 
                 28440 
                 34.69 
                 34.69 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 2.86 
                 5.72 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 0 
                 0.00 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 12.8 
                 12.80 
                 BM stim 
                 12.8 
               
               
                 osteo dif KC 
                   
                 0 
                 0.00 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 0 
                 0.00 
                 osteo undif 
                 0 
               
               
                 chondrocytes 
                   
                 4.78 
                 11.95 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 18.31 
                 18.31 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 0 
                 0.00 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 11.46 
                 22.92 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 0.87 
                 1.74 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 26.95 
                 53.90 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 18.91 
                 37.82 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 0 
                 0.00 
                 OA bone 
               
               
                   
                   
                   
                   
                 (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 8.66 
                 17.32 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 7.8 
                 15.60 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 16.93 
                 33.86 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 6.39 
                 12.78 
                 Cartilage 
                 −2.649452269 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 0 
                 0.00 
                 PBL 
               
               
                   
                   
                   
                   
                 unifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 1.15 
                 2.30 
                 PBL HIV 
                 2.3 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected (100%) 
                 29158 
                 0 
                 0.00 
                 MRC5 
               
               
                   
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 70.84 
                 141.68 
                 MRC5 HSV 
                 141.68 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 5.59 
                 11.18 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 0 
                 0.00 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg453915TECTORINa 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 −1.53 
               
               
                   
                 colon tumor 
                 5.57 
               
               
                   
                 colon tumor 
                 −1.44 
               
               
                   
                 colon tumor 
                 1.66 
               
               
                   
                 lung tumor 
                 −8.52 
               
               
                   
                 lung tumor 
                 −2.38 
               
               
                   
                 lung tumor 
                 6.58 
               
               
                   
                 lung tumor 
                 −3.85 
               
               
                   
                 breast tumor 
                 1.23 
               
               
                   
                 breast tumor 
                 27.92 
               
               
                   
                 breast tumor 
                 −14.42 
               
               
                   
                 breast tumor 
                 −11.62 
               
               
                   
                 brain stage 5 ALZ 
                 1.96 
               
               
                   
                 brain stage 5 ALZ 
                 2.76 
               
               
                   
                 brain stage 5 ALZ 
                 1.07 
               
               
                   
                 brain stage 5 ALZ 
                 1.67 
               
               
                   
                 lung 24 
                 −3.43 
               
               
                   
                 lung 28 
                 −4.29 
               
               
                   
                 lung 23 
                 −3.70 
               
               
                   
                 asthmatic lung 
                 1.14 
               
               
                   
                 asthmatic lung 
                 −2.58 
               
               
                   
                 asthmatic lung 
                 1.15 
               
               
                   
                 asthmatic lung 
                 2.80 
               
               
                   
                 endo VEGF 
                 −1.97 
               
               
                   
                 endo bFGF 
                 −2.52 
               
               
                   
                 heart T-1 
                 7.16 
               
               
                   
                 heart T-14 
                 0.00 
               
               
                   
                 heart T-3399 
                 0.00 
               
               
                   
                 BM stim 
                 12.80 
               
               
                   
                 osteo undif 
                 0.00 
               
               
                   
                 Cartilage (pool) 
                 −2.65 
               
               
                   
                 PBL HIV IIIB 
                 2.30 
               
               
                   
                 MRC5 HSV strain F 
                 141.68 
               
               
                   
                   
               
            
           
           
               
            
               
                 Gene Name SBh385630.antiinflam 
               
            
           
           
               
            
               
                 Some expression in adenoid, tonsils and T-cells suggesting a role in the immune system. Expression in GI 
               
               
                 tissues suggests a role in the digestive system and potential role in diseases of the GI system such as IBD. 
               
               
                 Overexpression in lung (1/4) and colon tumors (1/4) suggesting a role in lung and colon cancer. Increased 
               
               
                 expression in ischemic and dilated heart samples indicating a role in Cardiovascular diseases that are 
               
               
                 consistent with cardiac hypertrophy. Expression in whole brain but not localized to hypothalamus, cerebellum 
               
               
                 or cortex. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 SBh385630.antiinflam 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 0.00 
                 6.41 
                 3.21 
                 3.06 
                 16.34 
                 52.37 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 0.00 
                 0.00 
                 0.00 
                 0.96 
                 52.36 
                 0.00 
               
               
                 Zenbio 
               
               
                 Adrenal Gland Clontech 
                 8.40 
                 0.00 
                 4.20 
                 0.61 
                 81.97 
                 344.26 
               
               
                 Whole Brain Clontech 
                 817.17 
                 466.76 
                 641.97 
                 7.24 
                 6.91 
                 4433.46 
               
               
                 Fetal Brain Clontech 
                 3.80 
                 0.00 
                 1.90 
                 0.48 
                 103.95 
                 197.51 
               
               
                 Cerebellum Clontech 
                 6.66 
                 0.00 
                 3.33 
                 2.17 
                 23.04 
                 76.73 
               
               
                 Cervix 
                 11.99 
                 12.30 
                 12.15 
                 2.42 
                 20.66 
                 250.93 
               
               
                 Colon 
                 55.51 
                 211.32 
                 133.42 
                 2.71 
                 18.45 
                 2461.53 
               
               
                 Endometrium 
                 0.00 
                 0.00 
                 0.00 
                 0.73 
                 68.21 
                 0.00 
               
               
                 Esophagus 
                 11.75 
                 30.29 
                 21.02 
                 1.37 
                 36.50 
                 767.15 
               
               
                 Heart Clontech 
                 0.00 
                 0.00 
                 0.00 
                 1.32 
                 37.88 
                 0.00 
               
               
                 Hypothalamus 
                 0.00 
                 0.00 
                 0.00 
                 0.32 
                 155.28 
                 0.00 
               
               
                 Ileum 
                 40.37 
                 42.85 
                 41.61 
                 2.58 
                 19.38 
                 806.40 
               
               
                 Jejunum 
                 200.19 
                 263.82 
                 232.01 
                 6.60 
                 7.58 
                 1757.61 
               
               
                 Kidney 
                 18.38 
                 34.53 
                 26.46 
                 2.12 
                 23.58 
                 623.94 
               
               
                 Liver 
                 11.00 
                 17.20 
                 14.10 
                 1.50 
                 33.33 
                 470.00 
               
               
                 Fetal Liver Clontech 
                 150.74 
                 123.93 
                 137.34 
                 10.40 
                 4.81 
                 660.26 
               
               
                 Lung 
                 82.73 
                 77.24 
                 79.99 
                 2.57 
                 19.46 
                 1556.13 
               
               
                 Mammary Gland 
                 161.37 
                 155.19 
                 158.28 
                 13.00 
                 3.85 
                 608.77 
               
               
                 Clontech 
               
               
                 Myometrium 
                 5.79 
                 9.38 
                 7.59 
                 2.34 
                 21.37 
                 162.07 
               
               
                 Omentum 
                 36.14 
                 46.80 
                 41.47 
                 3.94 
                 12.69 
                 526.27 
               
               
                 Ovary 
                 59.25 
                 44.29 
                 51.77 
                 4.34 
                 11.52 
                 596.43 
               
               
                 Pancreas 
                 6.29 
                 6.70 
                 6.50 
                 0.81 
                 61.80 
                 401.42 
               
               
                 Head of Pancreas 
                 0.00 
                 26.25 
                 13.13 
                 1.57 
                 31.85 
                 417.99 
               
               
                 Parotid Gland 
                 8.77 
                 52.96 
                 30.87 
                 5.48 
                 9.12 
                 281.61 
               
               
                 Placenta Clontech 
                 4.11 
                 0.00 
                 2.06 
                 5.26 
                 9.51 
                 19.53 
               
               
                 Prostate 
                 100.91 
                 49.99 
                 75.45 
                 3.00 
                 16.67 
                 1257.50 
               
               
                 Rectum 
                 180.24 
                 305.61 
                 242.93 
                 1.23 
                 40.65 
                 9875.00 
               
               
                 Salivary Gland Clontech 
                 49.36 
                 70.01 
                 59.69 
                 7.31 
                 6.84 
                 408.24 
               
               
                 Skeletal Muscle 
                 0.00 
                 0.00 
                 0.00 
                 1.26 
                 39.68 
                 0.00 
               
               
                 Clontech 
               
               
                 Skin 
                 18.00 
                 3.22 
                 10.61 
                 1.21 
                 41.32 
                 438.43 
               
               
                 Small Intestine Clontech 
                 3.90 
                 2.55 
                 3.23 
                 0.98 
                 51.07 
                 164.71 
               
               
                 Spleen 
                 9.67 
                 5.60 
                 7.64 
                 4.92 
                 10.16 
                 77.59 
               
               
                 Stomach 
                 32.34 
                 83.60 
                 57.97 
                 2.73 
                 18.32 
                 1061.72 
               
               
                 Testis Clontech 
                 3.53 
                 0.00 
                 1.77 
                 0.57 
                 87.87 
                 155.10 
               
               
                 Thymus Clontech 
                 73.66 
                 60.02 
                 66.84 
                 9.89 
                 5.06 
                 337.92 
               
               
                 Thyroid 
                 15.87 
                 12.31 
                 14.09 
                 2.77 
                 18.05 
                 254.33 
               
               
                 Trachea Clontech 
                 98.68 
                 187.11 
                 142.90 
                 9.71 
                 5.15 
                 735.81 
               
               
                 Urinary Bladder 
                 118.92 
                 101.91 
                 110.42 
                 5.47 
                 9.14 
                 1009.28 
               
               
                 Uterus 
                 9.03 
                 24.21 
                 16.62 
                 5.34 
                 9.36 
                 155.62 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                 Mean 
                 copies of mRNA 
                   
                 Fold Change in 
               
               
                 Sample 
                 (GSK 
                 GOI 
                 detected/50 ng 
                   
                 Disease 
               
               
                 SBh385630.antiinflam 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 6479.77 
                 12959.54 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 7824.02 
                 15648.04 
                 colon tumor 
                 1.207453351 
               
               
                 colon normal GW98-178 
                 22080 
                 343.81 
                 687.62 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 3011.93 
                 6023.86 
                 colon tumor 
                 8.760449085 
               
               
                 colon normal GW98-561 
                 23514 
                 5457.38 
                 10914.76 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 4017.14 
                 8034.28 
                 colon tumor 
                 −1.358523726 
               
               
                 colon normal GW98-894 
                 24691 
                 14903.68 
                 29807.36 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 4814.19 
                 9628.38 
                 colon tumor 
                 −3.095781429 
               
               
                 lung normal GW98-3 
                 20742 
                 3731.84 
                 7463.68 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 719.6 
                 1439.20 
                 lung tumor 
                 −5.185992218 
               
               
                 lung normal GW97-179 
                 20677 
                 1090.56 
                 2181.12 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 6187.22 
                 12374.44 
                 lung tumor 
                 5.673433832 
               
               
                 lung normal GW98-165 
                 21922 
                 8416.82 
                 16833.64 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 4405.14 
                 8810.28 
                 lung tumor 
                 −1.910681613 
               
               
                 lung normal GW98-282 
                 22584 
                 2033.26 
                 4066.52 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 1785.69 
                 3571.38 
                 lung tumor 
                 −1.138641086 
               
               
                 breast normal GW00-392 
                 28750 
                 1583.49 
                 1583.49 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 1334.89 
                 2669.78 
                 breast tumor 
                 1.686010016 
               
               
                 breast normal GW00-413 
                 28798 
                 1225.92 
                 1225.92 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 1213.71 
                 2427.42 
                 breast tumor 
                 1.980080266 
               
               
                 breast normal GW00- 
                 27592-95 
                 862.26 
                 862.26 
                 breast 
               
               
                 235:238 
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00- 
                 27588-91 
                 1766.08 
                 1766.08 
                 breast tumor 
                 2.048198919 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 1420.57 
                 2841.14 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 760.05 
                 1520.10 
                 breast tumor 
                 −1.869048089 
               
               
                 brain normal BB99-542 
                 25507 
                 679.48 
                 1358.96 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 423.69 
                 847.38 
                 brain normal 
               
               
                 brain normal BB99-904 
                 25546 
                 401.34 
                 802.68 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 264.51 
                 529.02 
                 brain stage 5 
                 −1.895971167 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 648.88 
                 1297.76 
                 brain stage 5 
                 1.293869765 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 234.97 
                 469.94 
                 brain stage 5 
                 −2.134329205 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 404.55 
                 809.10 
                 brain stage 5 
                 −1.239657232 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 6620.85 
                 13241.70 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 320.43 
                 320.43 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 164.59 
                 164.59 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 141.57 
                 141.57 
                 lung 24 
                 −25.25392032 
               
               
                 lung 28 KC 
                 COPD 
                 323.8 
                 323.80 
                 lung 28 
                 −11.04137585 
               
               
                 lung 23 KC 
                 COPD 
                 363.35 
                 363.35 
                 lung 23 
                 −9.839541764 
               
               
                 lung 25 KC 
                 COPD 
                 574.07 
                 574.07 
                 lung 25 
               
               
                 asthmatic lung 
                 29321 
                 6073.99 
                 6073.99 
                 asthmatic 
                 1.698924325 
               
               
                 ODO3112 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29323 
                 4568.41 
                 9136.82 
                 asthmatic 
                 2.555612662 
               
               
                 ODO3433 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29322 
                 17389.11 
                 34778.22 
                 asthmatic 
                 9.727636026 
               
               
                 ODO3397 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29325 
                 4719.27 
                 9438.54 
                 asthmatic 
                 2.640005203 
               
               
                 ODO4928 
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 0 
                 0.00 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 0 
                 0.00 
                 endo VEGF 
                 0 
               
               
                 endo bFGF KC 
                   
                 0 
                 0.00 
                 endo bFGF 
                 0 
               
               
                 heart Clontech 
                 normal 
                 10.63 
                 21.26 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 599.01 
                 1198.02 
                 heart T-1 
                 56.3508937 
               
               
                 heart (T-14) non- 
                 29422 
                 666.41 
                 1332.82 
                 heart T-14 
                 62.69143932 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399) DCM 
                 29426 
                 142.85 
                 285.70 
                 heart T-3399 
                 13.43838194 
               
               
                 adenoid GW99-269 
                 26162 
                 1138 
                 2276.00 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 561.57 
                 1123.14 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 736.27 
                 1472.54 
                 T cells 
               
               
                 PBMNC KC 
                   
                 0 
                 0.00 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 30.38 
                 60.76 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 204.15 
                 408.30 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 57.66 
                 115.32 
                 dendritic 
               
               
                   
                   
                   
                   
                 cells 
               
               
                 neutrophils 
                 28440 
                 13.3 
                 13.30 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 5.71 
                 11.42 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 0 
                 0.00 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 50.38 
                 50.38 
                 BM stim 
                 50.38 
               
               
                 osteo dif KC 
                   
                 8.62 
                 8.62 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 0 
                 0.00 
                 osteo undif 
                 −8.62 
               
               
                 chondrocytes 
                   
                 14.98 
                 37.45 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 134.63 
                 134.63 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 73.89 
                 147.78 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 106.98 
                 213.96 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 26.59 
                 53.18 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 60.88 
                 121.76 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 60.81 
                 121.62 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 98.18 
                 98.18 
                 OA bone 
               
               
                   
                   
                   
                   
                 (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 78.3 
                 156.60 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 107.7 
                 215.40 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 72.21 
                 144.42 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 48.61 
                 97.22 
                 Cartilage 
                 −1.485496811 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 30.22 
                 60.44 
                 PBL unifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 21.89 
                 43.78 
                 PBL HIV 
                 −1.380539059 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected 
                 29158 
                 10.74 
                 21.48 
                 MRC5 
               
               
                 (100%) 
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 171.23 
                 342.46 
                 MRC5 HSV 
                 15.94320298 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 1143.85 
                 2287.70 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 388.06 
                 776.12 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name SBh385630.antiinflam 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 1.21 
               
               
                   
                 colon tumor 
                 8.76 
               
               
                   
                 colon tumor 
                 −1.36 
               
               
                   
                 colon tumor 
                 −3.10 
               
               
                   
                 lung tumor 
                 −5.19 
               
               
                   
                 lung tumor 
                 5.67 
               
               
                   
                 lung tumor 
                 −1.91 
               
               
                   
                 lung tumor 
                 −1.14 
               
               
                   
                 breast tumor 
                 1.69 
               
               
                   
                 breast tumor 
                 1.98 
               
               
                   
                 breast tumor 
                 2.05 
               
               
                   
                 breast tumor 
                 −1.87 
               
               
                   
                 brain stage 5 ALZ 
                 −1.90 
               
               
                   
                 brain stage 5 ALZ 
                 1.29 
               
               
                   
                 brain stage 5 ALZ 
                 −2.13 
               
               
                   
                 brain stage 5 ALZ 
                 −1.24 
               
               
                   
                 lung 24 
                 −25.25 
               
               
                   
                 lung 28 
                 −11.04 
               
               
                   
                 lung 23 
                 −9.84 
               
               
                   
                 asthmatic lung 
                 1.70 
               
               
                   
                 asthmatic lung 
                 2.56 
               
               
                   
                 asthmatic lung 
                 9.73 
               
               
                   
                 asthmatic lung 
                 2.64 
               
               
                   
                 endo VEGF 
                 0.00 
               
               
                   
                 endo bFGF 
                 0.00 
               
               
                   
                 heart T-1 
                 56.35 
               
               
                   
                 heart T-14 
                 62.69 
               
               
                   
                 heart T-3399 
                 13.44 
               
               
                   
                 BM stim 
                 50.38 
               
               
                   
                 osteo undif 
                 −8.62 
               
               
                   
                 Cartilage (pool) 
                 −1.49 
               
               
                   
                 PBL HIV IIIB 
                 −1.38 
               
               
                   
                 MRC5 HSV strain F 
                 15.94 
               
               
                   
                   
               
            
           
           
               
            
               
                 Gene Name sbg471005nAChR 
               
            
           
           
               
            
               
                 Expressed in immune cells with corroborating expression in OA and RA synovium suggesting a role in this disease. 
               
               
                 High expression in whole brain but not present in cortex, cerebellum, or hypothalamus suggesting localized brain 
               
               
                 expression. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 sbg471005nAChR 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 32.42 
                 2.90 
                 17.66 
                 3.06 
                 16.34 
                 288.56 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 0.00 
                 0.00 
                 0.00 
                 0.96 
                 52.36 
                 0.00 
               
               
                 Zenbio 
               
               
                 Adrenal Gland Clontech 
                 0.00 
                 0.00 
                 0.00 
                 0.61 
                 81.97 
                 0.00 
               
               
                 Whole Brain Clontech 
                 1606.00 
                 1058.07 
                 1332.04 
                 7.24 
                 6.91 
                 9199.14 
               
               
                 Fetal Brain Clontech 
                 0.00 
                 6.34 
                 3.17 
                 0.48 
                 103.95 
                 329.52 
               
               
                 Cerebellum Clontech 
                 10.65 
                 0.00 
                 5.33 
                 2.17 
                 23.04 
                 122.70 
               
               
                 Cervix 
                 0.00 
                 0.00 
                 0.00 
                 2.42 
                 20.66 
                 0.00 
               
               
                 Colon 
                 0.00 
                 0.00 
                 0.00 
                 2.71 
                 18.45 
                 0.00 
               
               
                 Endometrium 
                 0.00 
                 0.00 
                 0.00 
                 0.73 
                 68.21 
                 0.00 
               
               
                 Esophagus 
                 0.00 
                 2.52 
                 1.26 
                 1.37 
                 36.50 
                 45.99 
               
               
                 Heart Clontech 
                 4.05 
                 0.00 
                 2.03 
                 1.32 
                 37.88 
                 76.70 
               
               
                 Hypothalamus 
                 2.24 
                 0.00 
                 1.12 
                 0.32 
                 155.28 
                 173.91 
               
               
                 Ileum 
                 0.00 
                 0.00 
                 0.00 
                 2.58 
                 19.38 
                 0.00 
               
               
                 Jejunum 
                 20.32 
                 41.44 
                 30.88 
                 6.60 
                 7.58 
                 233.94 
               
               
                 Kidney 
                 14.56 
                 0.00 
                 7.28 
                 2.12 
                 23.58 
                 171.70 
               
               
                 Liver 
                 3.55 
                 10.72 
                 7.14 
                 1.50 
                 33.33 
                 237.83 
               
               
                 Fetal Liver Clontech 
                 127.95 
                 116.81 
                 122.38 
                 10.40 
                 4.81 
                 588.37 
               
               
                 Lung 
                 12.79 
                 0.00 
                 6.40 
                 2.57 
                 19.46 
                 124.42 
               
               
                 Mammary Gland 
                 30.53 
                 24.12 
                 27.33 
                 13.00 
                 3.85 
                 105.10 
               
               
                 Clontech 
               
               
                 Myometrium 
                 0.00 
                 7.10 
                 3.55 
                 2.34 
                 21.37 
                 75.85 
               
               
                 Omentum 
                 8.15 
                 0.00 
                 4.08 
                 3.94 
                 12.69 
                 51.71 
               
               
                 Ovary 
                 18.27 
                 7.02 
                 12.65 
                 4.34 
                 11.52 
                 145.68 
               
               
                 Pancreas 
                 0.00 
                 0.00 
                 0.00 
                 0.81 
                 61.80 
                 0.00 
               
               
                 Head of Pancreas 
                 0.00 
                 0.00 
                 0.00 
                 1.57 
                 31.85 
                 0.00 
               
               
                 Parotid Gland 
                 0.00 
                 0.00 
                 0.00 
                 5.48 
                 9.12 
                 0.00 
               
               
                 Placenta Clontech 
                 9.17 
                 0.00 
                 4.59 
                 5.26 
                 9.51 
                 43.58 
               
               
                 Prostate 
                 0.00 
                 1.35 
                 0.68 
                 3.00 
                 16.67 
                 11.25 
               
               
                 Rectum 
                 0.00 
                 0.00 
                 0.00 
                 1.23 
                 40.65 
                 0.00 
               
               
                 Salivary Gland 
                 0.00 
                 11.84 
                 5.92 
                 7.31 
                 6.84 
                 40.49 
               
               
                 Clontech 
               
               
                 Skeletal Muscle 
                 6.09 
                 7.36 
                 6.73 
                 1.26 
                 39.68 
                 266.87 
               
               
                 Clontech 
               
               
                 Skin 
                 0.00 
                 0.00 
                 0.00 
                 1.21 
                 41.32 
                 0.00 
               
               
                 Small Intestine 
                 0.00 
                 0.00 
                 0.00 
                 0.98 
                 51.07 
                 0.00 
               
               
                 Clontech 
               
               
                 Spleen 
                 5.20 
                 7.36 
                 6.28 
                 4.92 
                 10.16 
                 63.82 
               
               
                 Stomach 
                 12.85 
                 6.38 
                 9.62 
                 2.73 
                 18.32 
                 176.10 
               
               
                 Testis Clontech 
                 0.00 
                 2.25 
                 1.13 
                 0.57 
                 87.87 
                 98.86 
               
               
                 Thymus Clontech 
                 177.85 
                 168.23 
                 173.04 
                 9.89 
                 5.06 
                 874.82 
               
               
                 Thyroid 
                 6.44 
                 0.00 
                 3.22 
                 2.77 
                 18.05 
                 58.12 
               
               
                 Trachea Clontech 
                 5.07 
                 0.00 
                 2.54 
                 9.71 
                 5.15 
                 13.05 
               
               
                 Urinary Bladder 
                 0.00 
                 0.00 
                 0.00 
                 5.47 
                 9.14 
                 0.00 
               
               
                 Uterus 
                 29.20 
                 10.39 
                 19.80 
                 5.34 
                 9.36 
                 185.35 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                 Mean 
                 copies of mRNA 
                   
                 Fold Change 
               
               
                 Sample 
                 (GSK 
                 GOI 
                 detected/50 ng 
                   
                 in Disease 
               
               
                 sbg471005nAChR 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 1530.09 
                 3060.18 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 617.15 
                 1234.30 
                 colon tumor 
                 −2.479283805 
               
               
                 colon normal GW98-178 
                 22080 
                 406.03 
                 812.06 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 1231.53 
                 2463.06 
                 colon tumor 
                 3.033101002 
               
               
                 colon normal GW98-561 
                 23514 
                 844.37 
                 1688.74 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 633.99 
                 1267.98 
                 colon tumor 
                 −1.331834887 
               
               
                 colon normal GW98-894 
                 24691 
                 1130.51 
                 2261.02 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 721.29 
                 1442.58 
                 colon tumor 
                 −1.567344619 
               
               
                 lung normal GW98-3 
                 20742 
                 2433.65 
                 4867.30 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 334.04 
                 668.08 
                 lung tumor 
                 −7.28550473 
               
               
                 lung normal GW97-179 
                 20677 
                 823.51 
                 1647.02 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 1492 
                 2984.00 
                 lung tumor 
                 1.811756991 
               
               
                 lung normal GW98-165 
                 21922 
                 829.65 
                 1659.30 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 595.31 
                 1190.62 
                 lung tumor 
                 −1.393643648 
               
               
                 lung normal GW98-282 
                 22584 
                 357.69 
                 715.38 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 256.76 
                 513.52 
                 lung tumor 
                 −1.393090824 
               
               
                 breast normal GW00-392 
                 28750 
                 357.44 
                 357.44 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 280.98 
                 561.96 
                 breast tumor 
                 1.572179946 
               
               
                 breast normal GW00-413 
                 28798 
                 286.18 
                 286.18 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 195.5 
                 391.00 
                 breast tumor 
                 1.366272975 
               
               
                 breast normal GW00- 
                 27592-95 
                 161.68 
                 161.68 
                 breast 
               
               
                 235:238 
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00- 
                 27588-91 
                 217.83 
                 217.83 
                 breast tumor 
                 1.347290945 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 531.53 
                 1063.06 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 556.17 
                 1112.34 
                 breast tumor 
                 1.046356744 
               
               
                 brain normal BB99-542 
                 25507 
                 143.72 
                 287.44 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 569.17 
                 1138.34 
                 brain normal 
               
               
                 brain normal BB99-904 
                 25546 
                 106.85 
                 213.70 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 286.37 
                 572.74 
                 brain stage 5 
                 1.048027423 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 746.74 
                 1493.48 
                 brain stage 5 
                 2.732842121 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 382.97 
                 765.94 
                 brain stage 5 
                 1.401554151 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 367.49 
                 734.98 
                 brain stage 5 
                 1.344902042 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 175.41 
                 350.82 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 20.66 
                 20.66 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 13.06 
                 13.06 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 15.89 
                 15.89 
                 lung 24 
                 −6.182662052 
               
               
                 lung 28 KC 
                 COPD 
                 7.34 
                 7.34 
                 lung 28 
                 −13.38453678 
               
               
                 lung 23 KC 
                 COPD 
                 22.3 
                 22.30 
                 lung 23 
                 −4.405493274 
               
               
                 lung 25 KC 
                 COPD 
                 8.43 
                 8.43 
                 lung 25 
               
               
                 asthmatic lung 
                 29321 
                 264.47 
                 264.47 
                 asthmatic 
                 2.692012113 
               
               
                 ODO3112 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29323 
                 442.3 
                 884.60 
                 asthmatic 
                 9.004249688 
               
               
                 ODO3433 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29322 
                 670.04 
                 1340.08 
                 asthmatic 
                 13.64053236 
               
               
                 ODO3397 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29325 
                 414.13 
                 828.26 
                 asthmatic 
                 8.430770797 
               
               
                 ODO4928 
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 66.94 
                 66.94 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 18.49 
                 18.49 
                 endo VEGF 
                 −3.620335316 
               
               
                 endo bFGF KC 
                   
                 15.93 
                 15.93 
                 endo bFGF 
                 −4.202134338 
               
               
                 heart Clontech 
                 normal 
                 180.76 
                 361.52 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 161.9 
                 323.80 
                 heart T-1 
                 −1.116491662 
               
               
                 heart (T-14) non- 
                 29422 
                 141.03 
                 282.06 
                 heart T-14 
                 −1.281713111 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399) DCM 
                 29426 
                 321.32 
                 642.64 
                 heart T-3399 
                 1.777605665 
               
               
                 adenoid GW99-269 
                 26162 
                 193.61 
                 387.22 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 625.4 
                 1250.80 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 140.44 
                 280.88 
                 T cells 
               
               
                 PBMNC KC 
                   
                 0 
                 0.00 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 0 
                 0.00 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 476.72 
                 953.44 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 205.79 
                 411.58 
                 dendritic 
               
               
                   
                   
                   
                   
                 cells 
               
               
                 neutrophils 
                 28440 
                 1366.99 
                 1366.99 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 316.57 
                 633.14 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 29.41 
                 29.41 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 46.03 
                 46.03 
                 BM stim 
                 1.565113907 
               
               
                 osteo dif KC 
                   
                 17.47 
                 17.47 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 1.87 
                 1.87 
                 osteo undif 
                 −9.342245989 
               
               
                 chondrocytes 
                   
                 735.88 
                 1839.70 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 686.8 
                 686.80 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 4887.16 
                 9774.32 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 721.49 
                 1442.98 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 383.33 
                 766.66 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 780.94 
                 1561.88 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 543.62 
                 1087.24 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 780.12 
                 780.12 
                 OA bone 
               
               
                   
                   
                   
                   
                 (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 361.65 
                 723.30 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 197.57 
                 395.14 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 220.7 
                 441.40 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 75.52 
                 151.04 
                 Cartilage 
                 −2.922404661 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 1745.81 
                 3491.62 
                 PBL 
               
               
                   
                   
                   
                   
                 unifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 832.4 
                 1664.80 
                 PBL HIV 
                 −2.097321 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected 
                 29158 
                 147.92 
                 295.84 
                 MRC5 
               
               
                 (100%) 
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 146 
                 292.00 
                 MRC5 HSV 
                 −1.013150685 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 304.27 
                 608.54 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 139.44 
                 278.88 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg471005nAChR 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 −2.48 
               
               
                   
                 colon tumor 
                 3.03 
               
               
                   
                 colon tumor 
                 −1.33 
               
               
                   
                 colon tumor 
                 −1.57 
               
               
                   
                 lung tumor 
                 −7.29 
               
               
                   
                 lung tumor 
                 1.81 
               
               
                   
                 lung tumor 
                 −1.39 
               
               
                   
                 lung tumor 
                 −1.39 
               
               
                   
                 breast tumor 
                 1.57 
               
               
                   
                 breast tumor 
                 1.37 
               
               
                   
                 breast tumor 
                 1.35 
               
               
                   
                 breast tumor 
                 1.05 
               
               
                   
                 brain stage 5 ALZ 
                 1.05 
               
               
                   
                 brain stage 5 ALZ 
                 2.73 
               
               
                   
                 brain stage 5 ALZ 
                 1.40 
               
               
                   
                 brain stage 5 ALZ 
                 1.34 
               
               
                   
                 lung 24 
                 −6.18 
               
               
                   
                 lung 28 
                 −13.38 
               
               
                   
                 lung 23 
                 −4.41 
               
               
                   
                 asthmatic lung 
                 2.69 
               
               
                   
                 asthmatic lung 
                 9.00 
               
               
                   
                 asthmatic lung 
                 13.64 
               
               
                   
                 asthmatic lung 
                 8.43 
               
               
                   
                 endo VEGF 
                 −3.62 
               
               
                   
                 endo bFGF 
                 −4.20 
               
               
                   
                 heart T-1 
                 −1.12 
               
               
                   
                 heart T-14 
                 −1.28 
               
               
                   
                 heart T-3399 
                 1.78 
               
               
                   
                 BM stim 
                 1.57 
               
               
                   
                 osteo undif 
                 −9.34 
               
               
                   
                 Cartilage (pool) 
                 −2.92 
               
               
                   
                 PBL HIV IIIB 
                 −2.10 
               
               
                   
                 MRC5 HSV strain F 
                 −1.01 
               
               
                   
                   
               
            
           
           
               
            
               
                 Gene Name sbg442445PROa 
               
            
           
           
               
            
               
                 Strong expression in B-cells with expression in other immune cell types indicate function in immune system. 
               
               
                 Corroborating expression in RA and OA samples indicate role in disease. 2× increase in cells infected with 
               
               
                 HIV suggests possible marker in HIV infection. Expression in whole brain but not cortex or cerebellum suggests 
               
               
                 localized expression in brain. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 sbg442445PROa 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 1.13 
                 3.82 
                 2.48 
                 3.06 
                 16.34 
                 40.44 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 0.63 
                 0 
                 0.32 
                 0.96 
                 52.36 
                 16.49 
               
               
                 Zenbio 
               
               
                 Adrenal Gland Clontech 
                 0.64 
                 0.74 
                 0.69 
                 0.61 
                 81.97 
                 56.56 
               
               
                 Whole Brain Clontech 
                 368.87 
                 396.51 
                 382.69 
                 7.24 
                 6.91 
                 2642.89 
               
               
                 Fetal Brain Clontech 
                 1.57 
                 2.5 
                 2.04 
                 0.48 
                 103.95 
                 211.54 
               
               
                 Cerebellum Clontech 
                 1.63 
                 0 
                 0.82 
                 2.17 
                 23.04 
                 18.78 
               
               
                 Cervix 
                 4.57 
                 5.6 
                 5.09 
                 2.42 
                 20.66 
                 105.06 
               
               
                 Colon 
                 18.13 
                 7.38 
                 12.76 
                 2.71 
                 18.45 
                 235.33 
               
               
                 Endometrium 
                 4.23 
                 0 
                 2.12 
                 0.73 
                 68.21 
                 144.27 
               
               
                 Esophagus 
                 6.85 
                 12.66 
                 9.76 
                 1.37 
                 36.50 
                 356.02 
               
               
                 Heart Clontech 
                 12.83 
                 1.44 
                 7.14 
                 1.32 
                 37.88 
                 270.27 
               
               
                 Hypothalamus 
                 0.58 
                 7.26 
                 3.92 
                 0.32 
                 155.28 
                 608.70 
               
               
                 Ileum 
                 22.89 
                 6.34 
                 14.62 
                 2.58 
                 19.38 
                 283.24 
               
               
                 Jejunum 
                 6.67 
                 36.71 
                 21.69 
                 6.60 
                 7.58 
                 164.32 
               
               
                 Kidney 
                 2.82 
                 6.28 
                 4.55 
                 2.12 
                 23.58 
                 107.31 
               
               
                 Liver 
                 11.21 
                 1.24 
                 6.23 
                 1.50 
                 33.33 
                 207.50 
               
               
                 Fetal Liver Clontech 
                 118 
                 135.81 
                 126.91 
                 10.40 
                 4.81 
                 610.12 
               
               
                 Lung 
                 13.95 
                 37.87 
                 25.91 
                 2.57 
                 19.46 
                 504.09 
               
               
                 Mammary Gland 
                 15.77 
                 11.19 
                 13.48 
                 13.00 
                 3.85 
                 51.85 
               
               
                 Clontech 
               
               
                 Myometrium 
                 16.26 
                 49.21 
                 32.74 
                 2.34 
                 21.37 
                 699.47 
               
               
                 Omentum 
                 16.64 
                 25.59 
                 21.12 
                 3.94 
                 12.69 
                 267.96 
               
               
                 Ovary 
                 4.98 
                 7.48 
                 6.23 
                 4.34 
                 11.52 
                 71.77 
               
               
                 Pancreas 
                 1.23 
                 0 
                 0.62 
                 0.81 
                 61.80 
                 38.01 
               
               
                 Head of Pancreas 
                 3.57 
                 0 
                 1.79 
                 1.57 
                 31.85 
                 56.85 
               
               
                 Parotid Gland 
                 0.59 
                 0 
                 0.30 
                 5.48 
                 9.12 
                 2.69 
               
               
                 Placenta Clontech 
                 2.67 
                 2.75 
                 2.71 
                 5.26 
                 9.51 
                 25.76 
               
               
                 Prostate 
                 9.23 
                 7.92 
                 8.58 
                 3.00 
                 16.67 
                 142.92 
               
               
                 Rectum 
                 2.62 
                 4.28 
                 3.45 
                 1.23 
                 40.65 
                 140.24 
               
               
                 Salivary Gland 
                 1.02 
                 14.59 
                 7.81 
                 7.31 
                 6.84 
                 53.39 
               
               
                 Clontech 
               
               
                 Skeletal Muscle 
                 0 
                 0.98 
                 0.49 
                 1.26 
                 39.68 
                 19.44 
               
               
                 Clontech 
               
               
                 Skin 
                 2.72 
                 0 
                 1.36 
                 1.21 
                 41.32 
                 56.20 
               
               
                 Small Intestine 
                 0.99 
                 1 
                 1.00 
                 0.98 
                 51.07 
                 50.82 
               
               
                 Clontech 
               
               
                 Spleen 
                 31.29 
                 42.16 
                 36.73 
                 4.92 
                 10.16 
                 373.22 
               
               
                 Stomach 
                 15.74 
                   
                 7.87 
                 2.73 
                 18.32 
                 144.14 
               
               
                 Testis Clontech 
                 4.63 
                 111 
                 3.70 
                 0.57 
                 87.87 
                 325.13 
               
               
                 Thymus Clontech 
                 503.91 
                 615.6 
                 559.76 
                 9.89 
                 5.06 
                 2829.90 
               
               
                 Thyroid 
                 0.75 
                 10.38 
                 5.57 
                 2.77 
                 18.05 
                 100.45 
               
               
                 Trachea Clontech 
                 65.95 
                 52.98 
                 59.47 
                 9.71 
                 5.15 
                 306.20 
               
               
                 Urinary Bladder 
                 9.1 
                 3.76 
                 6.43 
                 5.47 
                 9.14 
                 58.78 
               
               
                 Uterus 
                 13.88 
                 4.35 
                 9.12 
                 5.34 
                 9.36 
                 85.35 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                 Mean 
                 copies of mRNA 
                   
                 Fold Change 
               
               
                 Sample 
                 (GSK 
                 GOI 
                 detected/50 ng 
                   
                 in Disease 
               
               
                 sbg442445PROa 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 392.89 
                 785.78 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 466.75 
                 933.50 
                 colon tumor 
                 1.18799155 
               
               
                 colon normal GW98-178 
                 22080 
                 113.54 
                 227.08 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 43.88 
                 87.76 
                 colon tumor 
                 −2.587511395 
               
               
                 colon normal GW98-561 
                 23514 
                 335.16 
                 670.32 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 173.85 
                 347.70 
                 colon tumor 
                 −1.927868852 
               
               
                 colon normal GW98-894 
                 24691 
                 288.76 
                 577.52 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 164.44 
                 328.88 
                 colon tumor 
                 −1.756020433 
               
               
                 lung normal GW98-3 
                 20742 
                 2119.16 
                 4238.32 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 33.63 
                 67.26 
                 lung tumor 
                 −63.01397562 
               
               
                 lung normal GW97-179 
                 20677 
                 1213.42 
                 2426.84 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 2011.79 
                 4023.58 
                 lung tumor 
                 1.657950256 
               
               
                 lung normal GW98-165 
                 21922 
                 2088.93 
                 4177.86 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 862.54 
                 1725.08 
                 lung tumor 
                 −2.421835509 
               
               
                 lung normal GW98-282 
                 22584 
                 499.54 
                 999.08 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 946.36 
                 1892.72 
                 lung tumor 
                 1.894462906 
               
               
                 breast normal GW00-392 
                 28750 
                 208.96 
                 208.96 
                 breast normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 259.34 
                 518.68 
                 breast tumor 
                 2.48219755 
               
               
                 breast normal GW00-413 
                 28798 
                 65.02 
                 65.02 
                 breast normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 493.02 
                 986.04 
                 breast tumor 
                 15.16517994 
               
               
                 breast normal GW00- 
                 27592-95 
                 24.18 
                 24.18 
                 breast normal 
               
               
                 235:238 
               
               
                 breast tumor GW00- 
                 27588-91 
                 126.63 
                 126.63 
                 breast tumor 
                 5.236972705 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 536.09 
                 1072.18 
                 breast normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 203.7 
                 407.40 
                 breast tumor 
                 −2.631762396 
               
               
                 brain normal BB99-542 
                 25507 
                 88.47 
                 176.94 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 147.87 
                 295.74 
                 brain normal 
               
               
                 brain normal BB99-904 
                 25546 
                 35.13 
                 70.26 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 75.02 
                 150.04 
                 brain stage 5 
                 −1.206211677 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 189 
                 378.00 
                 brain stage 5 
                 2.088628578 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 131.38 
                 262.76 
                 brain stage 5 
                 1.451873135 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 36.77 
                 73.54 
                 brain stage 5 
                 −2.46097362 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 1441.16 
                 2882.32 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 69.7 
                 69.70 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 59.95 
                 59.95 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 5.33 
                 5.33 
                 lung 24 
                 −142.0727017 
               
               
                 lung 28 KC 
                 COPD 
                 30.24 
                 30.24 
                 lung 28 
                 −25.04125331 
               
               
                 lung 23 KC 
                 COPD 
                 52.96 
                 52.96 
                 lung 23 
                 −14.29847998 
               
               
                 lung 25 KC 
                 COPD 
                 17.02 
                 17.02 
                 lung 25 
               
               
                 asthmatic lung 
                 29321 
                 309.94 
                 309.94 
                 asthmatic 
                 −2.44320675 
               
               
                 ODO3112 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29323 
                 532.32 
                 1064.64 
                 asthmatic 
                 1.405933991 
               
               
                 ODO3433 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29322 
                 1159.05 
                 2318.10 
                 asthmatic 
                 3.061218426 
               
               
                 ODO3397 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29325 
                 873.73 
                 1747.46 
                 asthmatic 
                 2.307647103 
               
               
                 ODO4928 
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 0 
                 0.00 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 0.93 
                 0.93 
                 endo VEGF 
                 0.93 
               
               
                 endo bFGF KC 
                   
                 5.16 
                 5.16 
                 endo bFGF 
                 5.16 
               
               
                 heart Clontech 
                 normal 
                 43.01 
                 86.02 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 81.55 
                 163.10 
                 heart T-1 
                 1.896070681 
               
               
                 heart (T-14) non- 
                 29422 
                 51.64 
                 103.28 
                 heart T-14 
                 1.200651011 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399) DCM 
                 29426 
                 90.27 
                 180.54 
                 heart T-3399 
                 2.098814229 
               
               
                 adenoid GW99-269 
                 26162 
                 982.05 
                 1964.10 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 3981.71 
                 7963.42 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 265.95 
                 531.90 
                 T cells 
               
               
                 PBMNC KC 
                   
                 40.89 
                 40.89 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 62.92 
                 125.84 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 9045.58 
                 18091.16 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 267.47 
                 534.94 
                 dendritic cells 
               
               
                 neutrophils 
                 28440 
                 1212.1 
                 1212.10 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 1563.76 
                 3127.52 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 56.55 
                 56.55 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 27.4 
                 27.40 
                 BM stim 
                 −2.063868613 
               
               
                 osteo dif KC 
                   
                 0 
                 0.00 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 0 
                 0.00 
                 osteo undif 
                 0 
               
               
                 chondrocytes 
                   
                 0.92 
                 2.30 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 524.44 
                 524.44 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 191.8 
                 383.60 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 461.09 
                 922.18 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 484.63 
                 969.26 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 698.08 
                 1396.16 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 1034.78 
                 2069.56 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 547.68 
                 547.68 
                 OA bone 
               
               
                   
                   
                   
                   
                 (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 286.6 
                 573.20 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 604.86 
                 1209.72 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 224.68 
                 449.36 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 113.78 
                 227.56 
                 Cartilage 
                 −1.974687994 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 966.68 
                 1933.36 
                 PBL 
               
               
                   
                   
                   
                   
                 unifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 1353.87 
                 2707.74 
                 PBL HIV 
                 1.400535855 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected 
                 29158 
                 1.28 
                 2.56 
                 MRC5 
               
               
                 (100%) 
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 34.07 
                 68.14 
                 MRC5 HSV 
                 26.6171875 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 3.55 
                 7.10 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 5.64 
                 11.28 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg442445PROa 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 1.19 
               
               
                   
                 colon tumor 
                 −2.59 
               
               
                   
                 colon tumor 
                 −1.93 
               
               
                   
                 colon tumor 
                 −1.76 
               
               
                   
                 lung tumor 
                 −63.01 
               
               
                   
                 lung tumor 
                 1.66 
               
               
                   
                 lung tumor 
                 −2.42 
               
               
                   
                 lung tumor 
                 1.89 
               
               
                   
                 breast tumor 
                 2.48 
               
               
                   
                 breast tumor 
                 15.17 
               
               
                   
                 breast tumor 
                 5.24 
               
               
                   
                 breast tumor 
                 −2.63 
               
               
                   
                 brain stage 5 ALZ 
                 −1.21 
               
               
                   
                 brain stage 5 ALZ 
                 2.09 
               
               
                   
                 brain stage 5 ALZ 
                 1.45 
               
               
                   
                 brain stage 5 ALZ 
                 −2.46 
               
               
                   
                 lung 24 
                 −142.07 
               
               
                   
                 lung 28 
                 −25.04 
               
               
                   
                 lung 23 
                 −14.30 
               
               
                   
                 asthmatic lung 
                 −2.44 
               
               
                   
                 asthmatic lung 
                 1.41 
               
               
                   
                 asthmatic lung 
                 3.06 
               
               
                   
                 asthmatic lung 
                 2.31 
               
               
                   
                 endo VEGF 
                 0.93 
               
               
                   
                 endo bFGF 
                 5.16 
               
               
                   
                 heart T-1 
                 1.90 
               
               
                   
                 heart T-14 
                 1.20 
               
               
                   
                 heart T-3399 
                 2.10 
               
               
                   
                 BM stim 
                 −2.06 
               
               
                   
                 osteo undif 
                 0.00 
               
               
                   
                 Cartilage (pool) 
                 −1.97 
               
               
                   
                 PBL HIV IIIB 
                 1.40 
               
               
                   
                 MRC5 HSV strain F 
                 26.62 
               
               
                   
                   
               
            
           
           
               
            
               
                 Gene Name sbg456548CytoRa 
               
            
           
           
               
            
               
                 Strongly expressed in adenoid/tonsils and dendritic cells. Overexpressed in stimulated bone marrow. Taken 
               
               
                 together, these data suggest a role in immune function. Expression in GI tract suggests potential role in 
               
               
                 diseases of the GI system like IBD, Chron&#39;s, etc. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 sbg456548CytoRa 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 0.00 
                 5.06 
                 2.53 
                 3.06 
                 16.34 
                 41.34 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 0.00 
                 0.00 
                 0.00 
                 0.96 
                 52.36 
                 0.00 
               
               
                 Zenbio 
               
               
                 Adrenal Gland Clontech 
                 0.00 
                 0.00 
                 0.00 
                 0.61 
                 81.97 
                 0.00 
               
               
                 Whole Brain Clontech 
                 0.00 
                 0.00 
                 0.00 
                 7.24 
                 6.91 
                 0.00 
               
               
                 Fetal Brain Clontech 
                 0.00 
                 0.00 
                 0.00 
                 0.48 
                 103.95 
                 0.00 
               
               
                 Cerebellum Clontech 
                 0.00 
                 0.00 
                 0.00 
                 2.17 
                 23.04 
                 0.00 
               
               
                 Cervix 
                 0.00 
                 7.86 
                 3.93 
                 2.42 
                 20.66 
                 81.20 
               
               
                 Colon 
                 9.12 
                 37.61 
                 23.37 
                 2.71 
                 18.45 
                 431.09 
               
               
                 Endometrium 
                 0.00 
                 0.00 
                 0.00 
                 0.73 
                 68.21 
                 0.00 
               
               
                 Esophagus 
                 0.00 
                 0.00 
                 0.00 
                 1.37 
                 36.50 
                 0.00 
               
               
                 Heart Clontech 
                 0.00 
                 0.00 
                 0.00 
                 1.32 
                 37.88 
                 0.00 
               
               
                 Hypothalamus 
                 0.00 
                 0.00 
                 0.00 
                 0.32 
                 155.28 
                 0.00 
               
               
                 Ileum 
                 not done 
                 39.63 
                 39.63 
                 2.58 
                 19.38 
                 768.02 
               
               
                 Jejunum 
                 9.16 
                 33.67 
                 21.42 
                 6.60 
                 7.58 
                 162.23 
               
               
                 Kidney 
                 0.00 
                 0.00 
                 0.00 
                 2.12 
                 23.58 
                 0.00 
               
               
                 Liver 
                 0.00 
                 13.75 
                 6.88 
                 1.50 
                 33.33 
                 229.17 
               
               
                 Fetal Liver Clontech 
                 0.00 
                 0.00 
                 0.00 
                 10.40 
                 4.81 
                 0.00 
               
               
                 Lung 
                 0.00 
                 0.00 
                 0.00 
                 2.57 
                 19.46 
                 0.00 
               
               
                 Mammary Gland 
                 136.73 
                 106.34 
                 121.54 
                 13.00 
                 3.85 
                 467.44 
               
               
                 Clontech 
               
               
                 Myometrium 
                 27.33 
                 17.56 
                 22.45 
                 2.34 
                 21.37 
                 479.59 
               
               
                 Omentum 
                 0.00 
                 12.61 
                 6.31 
                 3.94 
                 12.69 
                 80.01 
               
               
                 Ovary 
                 16.46 
                 17.90 
                 17.18 
                 4.34 
                 11.52 
                 197.93 
               
               
                 Pancreas 
                 0.00 
                 0.00 
                 0.00 
                 0.81 
                 61.80 
                 0.00 
               
               
                 Head of Pancreas 
                 0.00 
                 0.00 
                 0.00 
                 1.57 
                 31.85 
                 0.00 
               
               
                 Parotid Gland 
                 21.25 
                 23.72 
                 22.49 
                 5.48 
                 9.12 
                 205.16 
               
               
                 Placenta Clontech 
                 101.11 
                 73.40 
                 87.26 
                 5.26 
                 9.51 
                 829.42 
               
               
                 Prostate 
                 8.55 
                 0.00 
                 4.28 
                 3.00 
                 16.67 
                 71.25 
               
               
                 Rectum 
                 0.00 
                 0.00 
                 0.00 
                 1.23 
                 40.65 
                 0.00 
               
               
                 Salivary Gland 
                 0.00 
                 0.00 
                 0.00 
                 7.31 
                 6.84 
                 0.00 
               
               
                 Clontech 
               
               
                 Skeletal Muscle 
                 0.00 
                 0.00 
                 0.00 
                 1.26 
                 39.68 
                 0.00 
               
               
                 Clontech 
               
               
                 Skin 
                 0.00 
                 0.00 
                 0.00 
                 1.21 
                 41.32 
                 0.00 
               
               
                 Small Intestine 
                 0.00 
                 0.00 
                 0.00 
                 0.98 
                 51.07 
                 0.00 
               
               
                 Clontech 
               
               
                 Spleen 
                 31.60 
                 14.66 
                 23.13 
                 4.92 
                 10.16 
                 235.06 
               
               
                 Stomach 
                 0.00 
                 7.01 
                 3.51 
                 2.73 
                 18.32 
                 64.19 
               
               
                 Testis Clontech 
                 0.00 
                 0.00 
                 0.00 
                 0.57 
                 87.87 
                 0.00 
               
               
                 Thymus Clontech 
                 51.70 
                 103.21 
                 77.46 
                 9.89 
                 5.06 
                 391.58 
               
               
                 Thyroid 
                 0.00 
                 0.00 
                 0.00 
                 2.77 
                 18.05 
                 0.00 
               
               
                 Trachea Clontech 
                 0.00 
                 0.00 
                 0.00 
                 9.71 
                 5.15 
                 0.00 
               
               
                 Urinary Bladder 
                 0.00 
                 7.29 
                 3.65 
                 5.47 
                 9.14 
                 33.32 
               
               
                 Uterus 
                 5.98 
                 21.02 
                 13.50 
                 5.34 
                 9.36 
                 126.40 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                 Mean 
                 copies of mRNA 
                   
                 Fold Change in 
               
               
                 Sample 
                 (GSK 
                 GOI 
                 detected/50 ng 
                   
                 Disease 
               
               
                 sbg456548CytoRa 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 54.19 
                 108.38 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 242.87 
                 485.74 
                 colon tumor 
                 4.481823215 
               
               
                 colon normal GW98-178 
                 22080 
                 24.61 
                 49.22 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 17.37 
                 34.74 
                 colon tumor 
                 −1.416810593 
               
               
                 colon normal GW98-561 
                 23514 
                 120.13 
                 240.26 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 43.05 
                 86.10 
                 colon tumor 
                 −2.79047619 
               
               
                 colon normal GW98-894 
                 24691 
                 81.35 
                 162.70 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 16.94 
                 33.88 
                 colon tumor 
                 −4.802243211 
               
               
                 lung normal GW98-3 
                 20742 
                 12.83 
                 25.66 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 94.41 
                 188.82 
                 lung tumor 
                 7.358534684 
               
               
                 lung normal GW97-179 
                 20677 
                 519.7 
                 1039.40 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 46.83 
                 93.66 
                 lung tumor 
                 −11.09758702 
               
               
                 lung normal GW98-165 
                 21922 
                 7.95 
                 15.90 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 237.54 
                 475.08 
                 lung tumor 
                 29.87924528 
               
               
                 lung normal GW98-282 
                 22584 
                 251.04 
                 502.08 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 28.16 
                 56.32 
                 lung tumor 
                 −8.914772727 
               
               
                 breast normal GW00-392 
                 28750 
                 138.99 
                 138.99 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 147.66 
                 295.32 
                 breast tumor 
                 2.124757177 
               
               
                 breast normal GW00-413 
                 28798 
                 30.39 
                 30.39 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 37.64 
                 75.28 
                 breast tumor 
                 2.477130635 
               
               
                 breast normal GW00- 
                 27592-95 
                 218.09 
                 218.09 
                 breast 
               
               
                 235:238 
                   
                   
                   
                 normal 
               
               
                 breast tumor GW00- 
                 27588-91 
                 14.68 
                 14.68 
                 breast tumor 
                 −14.85626703 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 1888.3 
                 3776.60 
                 breast 
               
               
                   
                   
                   
                   
                 normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 877.2 
                 1754.40 
                 breast tumor 
                 −2.152644779 
               
               
                 brain normal BB99-542 
                 25507 
                 0 
                 0.00 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 0 
                 0.00 
                 brain normal 
               
               
                 brain normal BB99-904 
                 25546 
                 0 
                 0.00 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 0 
                 0.00 
                 brain stage 5 
                 0 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 7.32 
                 14.64 
                 brain stage 5 
                 14.64 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 0 
                 0.00 
                 brain stage 5 
                 0 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 0 
                 0.00 
                 brain stage 5 
                 0 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 10.31 
                 20.62 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 49.79 
                 49.79 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 4.11 
                 4.11 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 0.67 
                 0.67 
                 lung 24 
                 −38.10074627 
               
               
                 lung 28 KC 
                 COPD 
                 19.24 
                 19.24 
                 lung 28 
                 −1.326793139 
               
               
                 lung 23 KC 
                 COPD 
                 3.15 
                 3.15 
                 lung 23 
                 −8.103968254 
               
               
                 lung 25 KC 
                 COPD 
                 27.59 
                 27.59 
                 lung 25 
               
               
                 asthmatic lung 
                 29321 
                 2.95 
                 2.95 
                 asthmatic 
                 −8.653389831 
               
               
                 ODO3112 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29323 
                 9.86 
                 19.72 
                 asthmatic 
                 −1.294497972 
               
               
                 ODO3433 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29322 
                 24.39 
                 48.78 
                 asthmatic 
                 1.910880423 
               
               
                 ODO3397 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29325 
                 53.84 
                 107.68 
                 asthmatic 
                 4.218196063 
               
               
                 ODO4928 
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 0 
                 0.00 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 14.65 
                 14.65 
                 endo VEGF 
                 14.65 
               
               
                 endo bFGF KC 
                   
                 0 
                 0.00 
                 endo bFGF 
                 0 
               
               
                 heart Clontech 
                 normal 
                 0 
                 0.00 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 21.18 
                 42.36 
                 heart T-1 
                 42.36 
               
               
                 heart (T-14) non- 
                 29422 
                 27.4 
                 54.80 
                 heart T-14 
                 54.8 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399) DCM 
                 29426 
                 93.27 
                 186.54 
                 heart T-3399 
                 186.54 
               
               
                 adenoid GW99-269 
                 26162 
                 579.69 
                 1159.38 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 3780.08 
                 7560.16 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 5.86 
                 11.72 
                 T cells 
               
               
                 PBMNC KC 
                   
                 0 
                 0.00 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 0 
                 0.00 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 19.6 
                 39.20 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 580.67 
                 1161.34 
                 dendritic 
               
               
                   
                   
                   
                   
                 cells 
               
               
                 neutrophils 
                 28440 
                 19.76 
                 19.76 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 15.12 
                 30.24 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 0 
                 0.00 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 296.72 
                 296.72 
                 BM stim 
                 296.72 
               
               
                 osteo dif KC 
                   
                 0 
                 0.00 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 0 
                 0.00 
                 osteo undif 
                 0 
               
               
                 chondrocytes 
                   
                 15.31 
                 38.28 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 39.57 
                 39.57 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 0 
                 0.00 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 70.08 
                 140.16 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 23.73 
                 47.46 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 24.13 
                 48.26 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 51.88 
                 103.76 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 0 
                 0.00 
                 OA bone 
               
               
                   
                   
                   
                   
                 (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 0 
                 0.00 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 5.45 
                 10.90 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 0 
                 0.00 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 0 
                 0.00 
                 Cartilage 
                 0 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 76.67 
                 153.34 
                 PBLunifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 13.77 
                 27.54 
                 PBL HIV 
                 −5.567901235 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected 
                 29158 
                 0 
                 0.00 
                 MRC5 
               
               
                 (100%) 
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 0 
                 0.00 
                 MRC5 HSV 
                 0 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 0 
                 0.00 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 0 
                 0.00 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg456548CytoRa 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 4.48 
               
               
                   
                 colon tumor 
                 −1.42 
               
               
                   
                 colon tumor 
                 −2.79 
               
               
                   
                 colon tumor 
                 −4.80 
               
               
                   
                 lung tumor 
                 7.36 
               
               
                   
                 lung tumor 
                 −11.10 
               
               
                   
                 lung tumor 
                 29.88 
               
               
                   
                 lung tumor 
                 −8.91 
               
               
                   
                 breast tumor 
                 2.12 
               
               
                   
                 breast tumor 
                 2.48 
               
               
                   
                 breast tumor 
                 −14.86 
               
               
                   
                 breast tumor 
                 −2.15 
               
               
                   
                 brain stage 5 ALZ 
                 0.00 
               
               
                   
                 brain stage 5 ALZ 
                 14.64 
               
               
                   
                 brain stage 5 ALZ 
                 0.00 
               
               
                   
                 brain stage 5 ALZ 
                 0.00 
               
               
                   
                 lung 24 
                 −38.10 
               
               
                   
                 lung 28 
                 −1.33 
               
               
                   
                 lung 23 
                 −8.10 
               
               
                   
                 asthmatic lung 
                 −8.65 
               
               
                   
                 asthmatic lung 
                 −1.29 
               
               
                   
                 asthmatic lung 
                 1.91 
               
               
                   
                 asthmatic lung 
                 4.22 
               
               
                   
                 endo VEGF 
                 14.65 
               
               
                   
                 endo bFGF 
                 0.00 
               
               
                   
                 heart T-1 
                 42.36 
               
               
                   
                 heart T-14 
                 54.80 
               
               
                   
                 heart T-3399 
                 186.54 
               
               
                   
                 BM stim 
                 296.72 
               
               
                   
                 osteo undif 
                 0.00 
               
               
                   
                 Cartilage (pool) 
                 0.00 
               
               
                   
                 PBL HIV IIIB 
                 −5.57 
               
               
                   
                 MRC5 HSV strain F 
                 0.00 
               
               
                   
                   
               
            
           
           
               
            
               
                 Gene Name sbg442358PROa 
               
            
           
           
               
            
               
                 Expression in multiple immune cell types as well as stimulated bone marrow and thymus strongly suggests function 
               
               
                 in immune system. Overexpressed in breast tumors (1/4). Expression in RA and OA with corroborating expression in 
               
               
                 immune cells suggests role in these diseases. Overexpressed in heart disease suggesting role in CV diseases. 
               
               
                 Downregulated in HSV infected cells suggesting possible host cell factor. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Mean GOI 
                 Mean GOI 
                 Average 
                 18S 
                 50 ng/18S 
                 copies of mRNA 
               
               
                 Sample 
                 copies 
                 copies 
                 GOI 
                 rRNA 
                 rRNA 
                 detected/50 ng 
               
               
                 sbg442358PROa 
                 (sample 1) 
                 (sample 2) 
                 Copies 
                 (ng) 
                 (ng) 
                 total RNA 
               
               
                   
               
               
                 Subcutaneous 
                 1.86 
                 1.71 
                 1.79 
                 3.06 
                 16.34 
                 29.17 
               
               
                 Adipocytes Zenbio 
               
               
                 Subcutaneous Adipose 
                 0.71 
                 0.73 
                 0.72 
                 0.96 
                 52.36 
                 37.70 
               
               
                 Zenbio 
               
               
                 Adrenal Gland Clontech 
                 3.45 
                 1.89 
                 2.67 
                 0.61 
                 81.97 
                 218.85 
               
               
                 Whole Brain Clontech 
                 406.27 
                 496.60 
                 451.44 
                 7.24 
                 6.91 
                 3117.65 
               
               
                 Fetal Brain Clontech 
                 3.82 
                 1.68 
                 2.75 
                 0.48 
                 103.95 
                 285.86 
               
               
                 Cerebellum Clontech 
                 5.84 
                 30.51 
                 18.18 
                 2.17 
                 23.04 
                 418.78 
               
               
                 Cervix 
                 2.50 
                 0.48 
                 1.49 
                 2.42 
                 20.66 
                 30.79 
               
               
                 Colon 
                 18.45 
                 18.77 
                 18.61 
                 2.71 
                 18.45 
                 343.36 
               
               
                 Endometrium 
                 4.93 
                 0.30 
                 2.62 
                 0.73 
                 68.21 
                 178.38 
               
               
                 Esophagus 
                 8.97 
                 6.99 
                 7.98 
                 1.37 
                 36.50 
                 291.24 
               
               
                 Heart Clontech 
                 5.26 
                 16.53 
                 10.90 
                 1.32 
                 37.88 
                 412.69 
               
               
                 Hypothalamus 
                 2.10 
                 2.41 
                 2.26 
                 0.32 
                 155.28 
                 350.16 
               
               
                 Ileum 
                 18.94 
                 12.62 
                 15.78 
                 2.58 
                 19.38 
                 305.81 
               
               
                 Jejunum 
                 65.51 
                 95.24 
                 80.38 
                 6.60 
                 7.58 
                 608.90 
               
               
                 Kidney 
                 2.60 
                 3.81 
                 3.21 
                 2.12 
                 23.58 
                 75.59 
               
               
                 Liver 
                 7.19 
                 7.05 
                 7.12 
                 1.50 
                 33.33 
                 237.33 
               
               
                 Fetal Liver Clontech 
                 1252.22 
                 1363.06 
                 1307.64 
                 10.40 
                 4.81 
                 6286.73 
               
               
                 Lung 
                 27.57 
                 6.97 
                 17.27 
                 2.57 
                 19.46 
                 335.99 
               
               
                 Mammary Gland 
                 79.83 
                 72.99 
                 76.41 
                 13.00 
                 3.85 
                 293.88 
               
               
                 Clontech 
               
               
                 Myometrium 
                 2.46 
                 10.62 
                 6.54 
                 2.34 
                 21.37 
                 139.74 
               
               
                 Omentum 
                 10.40 
                 3.27 
                 6.84 
                 3.94 
                 12.69 
                 86.74 
               
               
                 Ovary 
                 17.71 
                 31.15 
                 24.43 
                 4.34 
                 11.52 
                 281.45 
               
               
                 Pancreas 
                 3.33 
                 1.74 
                 2.54 
                 0.81 
                 61.80 
                 156.67 
               
               
                 Head of Pancreas 
                 3.82 
                 6.17 
                 5.00 
                 1.57 
                 31.85 
                 159.08 
               
               
                 Parotid Gland 
                 22.77 
                 22.54 
                 22.66 
                 5.48 
                 9.12 
                 206.71 
               
               
                 Placenta Clontech 
                 14.71 
                 53.83 
                 34.27 
                 5.26 
                 9.51 
                 325.76 
               
               
                 Prostate 
                 16.71 
                 19.39 
                 18.05 
                 3.00 
                 16.67 
                 300.83 
               
               
                 Rectum 
                 6.71 
                 3.49 
                 5.10 
                 1.23 
                 40.65 
                 207.32 
               
               
                 Salivary Gland 
                 55.38 
                 9.30 
                 32.34 
                 7.31 
                 6.84 
                 221.20 
               
               
                 Clontech 
               
               
                 Skeletal Muscle 
                 3.79 
                 4.16 
                 3.98 
                 1.26 
                 39.68 
                 157.74 
               
               
                 Clontech 
               
               
                 Skin 
                 4.51 
                 14.47 
                 9.49 
                 1.21 
                 41.32 
                 392.15 
               
               
                 Small Intestine 
                 8.12 
                 7.87 
                 8.00 
                 0.98 
                 51.07 
                 408.32 
               
               
                 Clontech 
               
               
                 Spleen 
                 14.88 
                 17.12 
                 16.00 
                 4.92 
                 10.16 
                 162.60 
               
               
                 Stomach 
                 21.85 
                 11.68 
                 16.77 
                 2.73 
                 18.32 
                 307.05 
               
               
                 Testis Clontech 
                 22.77 
                 11.54 
                 17.16 
                 0.57 
                 87.87 
                 1507.47 
               
               
                 Thymus Clontech 
                 1990.82 
                 1374.71 
                 1682.77 
                 9.89 
                 5.06 
                 8507.41 
               
               
                 Thyroid 
                 16.85 
                 2.86 
                 9.86 
                 2.77 
                 18.05 
                 177.89 
               
               
                 Trachea Clontech 
                 29.69 
                 82.85 
                 56.27 
                 9.71 
                 5.15 
                 289.75 
               
               
                 Urinary Bladder 
                 2.32 
                 13.42 
                 7.87 
                 5.47 
                 9.14 
                 71.94 
               
               
                 Uterus 
                 8.86 
                 11.18 
                 10.02 
                 5.34 
                 9.36 
                 93.82 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Reg number 
                 Mean 
                 copies of mRNA 
                   
                 Fold Change 
               
               
                 Sample 
                 (GSK 
                 GOI 
                 detected/50 ng 
                   
                 in Disease 
               
               
                 sbg442358PROa 
                 identifier) 
                 copies 
                 total RNA 
                 Sample 
                 Population 
               
               
                   
               
               
                 colon normal GW98-167 
                 21941 
                 1232.32 
                 2464.64 
                 colon normal 
                   
               
               
                 colon tumor GW98-166 
                 21940 
                 2940.17 
                 5880.34 
                 colon tumor 
                 2.385881914 
               
               
                 colon normal GW98-178 
                 22080 
                 221.26 
                 442.52 
                 colon normal 
               
               
                 colon tumor GW98-177 
                 22060 
                 709.52 
                 1419.04 
                 colon tumor 
                 3.20672512 
               
               
                 colon normal GW98-561 
                 23514 
                 985.52 
                 1971.04 
                 colon normal 
               
               
                 colon tumor GW98-560 
                 23513 
                 829.67 
                 1659.34 
                 colon tumor 
                 −1.18784577 
               
               
                 colon normal GW98-894 
                 24691 
                 2738.17 
                 5476.34 
                 colon normal 
               
               
                 colon tumor GW98-893 
                 24690 
                 3022.06 
                 6044.12 
                 colon tumor 
                 1.103678734 
               
               
                 lung normal GW98-3 
                 20742 
                 536.82 
                 1073.64 
                 lung normal 
               
               
                 lung tumor GW98-2 
                 20741 
                 594.2 
                 1188.40 
                 lung tumor 
                 1.106888715 
               
               
                 lung normal GW97-179 
                 20677 
                 4382.61 
                 8765.22 
                 lung normal 
               
               
                 lung tumor GW97-178 
                 20676 
                 359.07 
                 718.14 
                 lung tumor 
                 −12.20544741 
               
               
                 lung normal GW98-165 
                 21922 
                 622.06 
                 1244.12 
                 lung normal 
               
               
                 lung tumor GW98-164 
                 21921 
                 1299.85 
                 2599.70 
                 lung tumor 
                 2.089589429 
               
               
                 lung normal GW98-282 
                 22584 
                 1782.09 
                 3564.18 
                 lung normal 
               
               
                 lung tumor GW98-281 
                 22583 
                 470.51 
                 941.02 
                 lung tumor 
                 −3.787570934 
               
               
                 breast normal GW00-392 
                 28750 
                 429 
                 429.00 
                 breast normal 
               
               
                 breast tumor GW00-391 
                 28746 
                 417.99 
                 835.98 
                 breast tumor 
                 1.948671329 
               
               
                 breast normal GW00-413 
                 28798 
                 16.03 
                 16.03 
                 breast normal 
               
               
                 breast tumor GW00-412 
                 28797 
                 1048.11 
                 2096.22 
                 breast tumor 
                 130.768559 
               
               
                 breast normal GW00- 
                 27592-95 
                 2.17 
                 2.17 
                 breast normal 
               
               
                 235:238 
               
               
                 breast tumor GW00- 
                 27588-91 
                 69.91 
                 69.91 
                 breast tumor 
                 32.21658986 
               
               
                 231:234 
               
               
                 breast normal GW98-621 
                 23656 
                 1037.08 
                 2074.16 
                 breast normal 
               
               
                 breast tumor GW98-620 
                 23655 
                 1010.59 
                 2021.18 
                 breast tumor 
                 −1.026212411 
               
               
                 brain normal BB99-542 
                 25507 
                 299.28 
                 598.56 
                 brain normal 
               
               
                 brain normal BB99-406 
                 25509 
                 250.85 
                 501.70 
                 brain normal 
               
               
                 brain normal BB99-904 
                 25546 
                 97.7 
                 195.40 
                 brain normal 
               
               
                 brain stage 5 ALZ BB99- 
                 25502 
                 125 
                 250.00 
                 brain stage 5 
                 −1.727546667 
               
               
                 874 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25503 
                 850.01 
                 1700.02 
                 brain stage 5 
                 3.936264143 
               
               
                 887 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25504 
                 347.91 
                 695.82 
                 brain stage 5 
                 1.611117114 
               
               
                 862 
                   
                   
                   
                 ALZ 
               
               
                 brain stage 5 ALZ BB99- 
                 25542 
                 147.11 
                 294.22 
                 brain stage 5 
                 −1.467903836 
               
               
                 927 
                   
                   
                   
                 ALZ 
               
               
                 CT lung KC 
                 normal 
                 130.37 
                 260.74 
                 CT lung 
               
               
                 lung 26 KC 
                 normal 
                 159.19 
                 159.19 
                 lung 26 
               
               
                 lung 27 KC 
                 normal 
                 0.49 
                 0.49 
                 lung 27 
               
               
                 lung 24 KC 
                 COPD 
                 2.37 
                 2.37 
                 lung 24 
                 −47.89873418 
               
               
                 lung 28 KC 
                 COPD 
                 45.72 
                 45.72 
                 lung 28 
                 −2.482939633 
               
               
                 lung 23 KC 
                 COPD 
                 20.36 
                 20.36 
                 lung 23 
                 −5.575638507 
               
               
                 lung 25 KC 
                 COPD 
                 33.66 
                 33.66 
                 lung 25 
               
               
                 asthmatic lung 
                 29321 
                 65.46 
                 65.46 
                 asthmatic 
                 −1.734188818 
               
               
                 ODO3112 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29323 
                 532.42 
                 1064.84 
                 asthmatic 
                 9.380197322 
               
               
                 ODO3433 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29322 
                 2865.67 
                 5731.34 
                 asthmatic 
                 50.48749119 
               
               
                 ODO3397 
                   
                   
                   
                 lung 
               
               
                 asthmatic lung 
                 29325 
                 494.27 
                 988.54 
                 asthmatic 
                 8.708069063 
               
               
                 ODO4928 
                   
                   
                   
                 lung 
               
               
                 endo cells KC 
                 control 
                 62.77 
                 62.77 
                 endo cells 
               
               
                 endo VEGF KC 
                   
                 22.41 
                 22.41 
                 endo VEGF 
                 −2.800981705 
               
               
                 endo bFGF KC 
                   
                 33.16 
                 33.16 
                 endo bFGF 
                 −1.892943305 
               
               
                 heart Clontech 
                 normal 
                 74.18 
                 148.36 
                 heart 
               
               
                 heart (T-1) ischemic 
                 29417 
                 270.07 
                 540.14 
                 heart T-1 
                 3.640738744 
               
               
                 heart (T-14) non- 
                 29422 
                 680.12 
                 1360.24 
                 heart T-14 
                 9.168509032 
               
               
                 obstructive DCM 
               
               
                 heart (T-3399) DCM 
                 29426 
                 414 
                 828.00 
                 heart T-3399 
                 5.581019143 
               
               
                 adenoid GW99-269 
                 26162 
                 781.46 
                 1562.92 
                 adenoid 
               
               
                 tonsil GW98-280 
                 22582 
                 2279.13 
                 4558.26 
                 tonsil 
               
               
                 T cells PC00314 
                 28453 
                 1129.27 
                 2258.54 
                 T cells 
               
               
                 PBMNC KC 
                   
                 27.98 
                 27.98 
                 PBMNC 
               
               
                 monocyte KC 
                   
                 3.55 
                 7.10 
                 monocyte 
               
               
                 B cells PC00665 
                 28455 
                 872.58 
                 1745.16 
                 B cells 
               
               
                 dendritic cells 28441 
                   
                 1055.22 
                 2110.44 
                 dendritic cells 
               
               
                 neutrophils 
                 28440 
                 740.39 
                 740.39 
                 neutrophils 
               
               
                 eosinophils 
                 28446 
                 1081.83 
                 2163.66 
                 eosinophils 
               
               
                 BM unstim KC 
                   
                 50.91 
                 50.91 
                 BM unstim 
               
               
                 BM stim KC 
                   
                 391.11 
                 391.11 
                 BM stim 
                 7.682380672 
               
               
                 osteo dif KC 
                   
                 161.31 
                 161.31 
                 osteo dif 
               
               
                 osteo undif KC 
                   
                 40.01 
                 40.01 
                 osteo undif 
                 −4.031742064 
               
               
                 chondrocytes 
                   
                 2250.59 
                 5626.48 
                 chondrocytes 
               
               
                 OA Synovium IP12/01 
                 29462 
                 229.19 
                 229.19 
                 OA Synovium 
               
               
                 OA Synovium NP10/01 
                 29461 
                 152.3 
                 304.60 
                 OA Synovium 
               
               
                 OA Synovium NP57/00 
                 28464 
                 413.06 
                 826.12 
                 OA Synovium 
               
               
                 RA Synovium NP03/01 
                 28466 
                 611.02 
                 1222.04 
                 RA Synovium 
               
               
                 RA Synovium NP71/00 
                 28467 
                 385.94 
                 771.88 
                 RA Synovium 
               
               
                 RA Synovium NP45/00 
                 28475 
                 1701.68 
                 3403.36 
                 RA Synovium 
               
               
                 OA bone (biobank) 
                 29217 
                 225.69 
                 225.69 
                 OA bone 
               
               
                   
                   
                   
                   
                 (biobank) 
               
               
                 OA bone Sample 1 
                 J. Emory 
                 306.63 
                 613.26 
                 OA bone 
               
               
                 OA bone Sample 2 
                 J. Emory 
                 1811.32 
                 3622.64 
                 OA bone 
               
               
                 Cartilage (pool) 
                 Normal 
                 384.44 
                 768.88 
                 Cartilage 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 Cartilage (pool) 
                 OA 
                 174.53 
                 349.06 
                 Cartilage 
                 −2.202715865 
               
               
                   
                   
                   
                   
                 (pool) 
               
               
                 PBL unifected 
                 28441 
                 9016.82 
                 18033.64 
                 PBL 
               
               
                   
                   
                   
                   
                 unifected 
               
               
                 PBL HIV IIIB 
                 28442 
                 4331.76 
                 8663.52 
                 PBL HIV 
                 −2.081560382 
               
               
                   
                   
                   
                   
                 IIIB 
               
               
                 MRC5 uninfected 
                 29158 
                 2232.48 
                 4464.96 
                 MRC5 
               
               
                 (100%) 
                   
                   
                   
                 uninfected 
               
               
                   
                   
                   
                   
                 (100%) 
               
               
                 MRC5 HSV strain F 
                 29178 
                 419.67 
                 839.34 
                 MRC5 HSV 
                 −5.319608264 
               
               
                   
                   
                   
                   
                 strain F 
               
               
                 W12 cells 
                 29179 
                 3336.07 
                 6672.14 
                 W12 cells 
               
               
                 Keratinocytes 
                 29180 
                 5568.91 
                 11137.82 
                 Keratinocytes 
               
               
                   
               
            
           
           
               
            
               
                 Gene Name sbg442358PROa 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fold Change in Disease 
               
               
                   
                 Disease tissues 
                 Population Relative to Normal 
               
               
                   
                   
               
               
                   
                 colon tumor 
                 2.39 
               
               
                   
                 colon tumor 
                 3.21 
               
               
                   
                 colon tumor 
                 −1.19 
               
               
                   
                 colon tumor 
                 1.10 
               
               
                   
                 lung tumor 
                 1.11 
               
               
                   
                 lung tumor 
                 −12.21 
               
               
                   
                 lung tumor 
                 2.09 
               
               
                   
                 lung tumor 
                 −3.79 
               
               
                   
                 breast tumor 
                 1.95 
               
               
                   
                 breast tumor 
                 130.77 
               
               
                   
                 breast tumor 
                 32.22 
               
               
                   
                 breast tumor 
                 −1.03 
               
               
                   
                 brain stage 5 ALZ 
                 −1.73 
               
               
                   
                 brain stage 5 ALZ 
                 3.94 
               
               
                   
                 brain stage 5 ALZ 
                 1.61 
               
               
                   
                 brain stage 5 ALZ 
                 −1.47 
               
               
                   
                 lung 24 
                 −47.90 
               
               
                   
                 lung 28 
                 −2.48 
               
               
                   
                 lung 23 
                 −5.58 
               
               
                   
                 asthmatic lung 
                 −1.73 
               
               
                   
                 asthmatic lung 
                 9.38 
               
               
                   
                 asthmatic lung 
                 50.49 
               
               
                   
                 asthmatic lung 
                 8.71 
               
               
                   
                 endo VEGF 
                 −2.80 
               
               
                   
                 endo bFGF 
                 −1.89 
               
               
                   
                 heart T-1 
                 3.64 
               
               
                   
                 heart T-14 
                 9.17 
               
               
                   
                 heart T-3399 
                 5.58 
               
               
                   
                 BM stim 
                 7.68 
               
               
                   
                 osteo undif 
                 −4.03 
               
               
                   
                 Cartilage (pool) 
                 −2.20 
               
               
                   
                 PBL HIV IIIB 
                 −2.08 
               
               
                   
                 MRC5 HSV strain F 
                 −5.32 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE V 
               
             
            
               
                   
               
               
                   
               
               
                 Additional diseases based on mRNA expression in specific tissues 
               
            
           
           
               
               
            
               
                 Tissue 
                   
               
               
                 Expression 
                 Additional Diseases 
               
               
                   
               
               
                 Brain 
                 Neurological and psychiatric diseases, including 
               
               
                   
                 Alzheimers, parasupranuclear palsey, Huntington&#39;s disease, 
               
               
                   
                 myotonic dystrophy, anorexia, depression, schizophrenia, 
               
               
                   
                 headache, amnesias, anxiety disorders, sleep disorders, 
               
               
                   
                 multiple sclerosis 
               
               
                 Heart 
                 Cardiovascular diseases, including congestive heart 
               
               
                   
                 failure, dilated cardiomyopathy, cardiac arrhythmias, 
               
               
                   
                 Hodgson&#39;s Disease, myocardial infarction, cardiac 
               
               
                   
                 arrhythmias 
               
               
                 Lung 
                 Respiratory diseases, including asthma, Chronic 
               
               
                   
                 Obstructive Pulmonary Disease, cystic fibrosis, acute 
               
               
                   
                 bronchitis, adult respiratory distress syndrome 
               
               
                 Liver 
                 Dyslipidemia, hypercholesterolemia, hypertriglyceridemia, 
               
               
                   
                 cirrhosis, hepatic encephalopathy, fatty hepatocirrhosis, 
               
               
                   
                 viral and nonviral hepatitis, Type II Diabetes Mellitis, 
               
               
                   
                 impaired glucose tolerance 
               
               
                 Kidney 
                 Renal diseases, including acute and chronic renal failure, 
               
               
                   
                 acute tubular necrosis, cystinuria, Fanconi&#39;s Syndrome, 
               
               
                   
                 glomerulonephritis, renal cell carcinoma, renovascular 
               
               
                   
                 hypertension 
               
               
                 Skeletal 
                 Eulenburg&#39;s Disease, hypoglycemia, obesity, tendinitis, 
               
               
                 muscle 
                 periodic paralyses, malignant hyperthermia, paramyotonia 
               
               
                   
                 congenita, myotonia congenita 
               
               
                 Intestine 
                 Gastrointestinal diseases, including Myotonia congenita, 
               
               
                   
                 Ileus, Intestinal Obstruction, Tropical Sprue, 
               
               
                   
                 Pseudomembranous Enterocolitis 
               
               
                 Spleen/ 
                 Lymphangiectasia, hypersplenism, angiomas, ankylosing 
               
               
                 lymph 
                 spondylitis, Hodgkin&#39;s Disease, macroglobulinemia, 
               
               
                   
                 malignant lymphomas, rheumatoid arthritis 
               
               
                 Placenta 
                 Choriocarcinoma, hydatidiform mole, placenta previa 
               
               
                 Testis 
                 Testicular cancer, male reproductive diseases, including 
               
               
                   
                 low testosterone and male infertility 
               
               
                 Pancreas 
                 Diabetic ketoacidosis, Type 1 &amp; 2 diabetes, obesity, 
               
               
                   
                 impaired glucose tolerance 
               
               
                   
               
            
           
         
       
     
     EXAMPLE  
     Enhanced Would Repair Mediated by sbg453915TECTORINa Homolog  
      Polypeptides involved in basement membrane matrix formation and survival, proliferation and/or differentiation of cells involved in cellular regeneration and wound or joint repair are of interest. Previously, connective tissue factors such as collagen, fibronectin, laminin polypeptides and others are known to be involved in wound repair. In addition, growth and differentiation factors such as VEGF, PDGF and members of the bone morphogenic protein family have been shown to be important in the overall tissue regeneration process. Therefore, novel polypeptides, which are related to or have the function of connective tissue proteins or growth factors or protein, which may have combined functionality of a connective tissue factor and a growth factor, are of interest. β-Tectorin is a naturally occurring compound that is produced in the inner ear during development and embryo growth. The function of β-Tectorin is not well understood but it is proposed to aid in the transmittal of sound from the eardrum through pressure sensitive ion channels (Legan et al. (1997) J. Biol. Chem. 272(13):8791-8801).  
      β-Tectorin is thus useful for treating conditions in which enhanced wound repair is required, for example, diabetic ulcers and vascular injuries resulting from trauma such as subcutaneous wounds. Being a non-collagenous connective tissue factor β-Tectorin enhances connective tissue matrixes allowing other cells and factors to nucleate on the β-Tectorin and thus lead to a stronger and faster deposition of other matrix proteins within the wound. β-Tectorin may also aid in the growth or formation of new or stronger blood vessels a process known as vascular neogenesis or angiogenesis. In addition, β-Tectorin may aid in the repair of damaged blood vessels within the wound or joint a process known as vasculogenesis. β-Tectorin would also find use in the regrowth and restoration of cartilage tissue in osteo or rheumatoid arthritic joints as well as other uses that can be deduced by a person knowledgeable in the art. The polypeptide encoded by sbg453915TECTORINa (SEQ ID NO:35) is the human homolog of mouse β-Tectorin (SEQ ID NO:46). The human and murine polypeptide homologs are 94.2% identical.  
      The ability of β-Tectorin to mediate wound repair was demonstrated in a murine model system using an adenoviral expression system to express the polypeptide in vivo. The open reading frame (ORF) of the gene encoding the murine β-Tectorin (SEQ ID NO:45; referred to herein as MPA190) was subcloned into the adenovirus shuttle vector pShuttle (ClonTech) using appropriate restriction sites, placing the ORF downstream of the CMV IE promoter in the correct orientation. An I-CeuI/PI-SceI fragment containing the expression cassette (CMV IE-ORF-BGH polyA) was isolated from the shuttle vector and was swapped with a GFP expression cassette driven by bacterial Lac promoter at the I-CeuI/PI-SceI sites of the adenovirus backbone plasmid pAdX derived from pAdeno-X (ClonTech). The cloning step was carried by a convenient green/white selection process, in which white colonies contained the recombinant construct, pAdX.MPA190. The purified molecular clone DNA of adenovirus vector was linearized by digesting with restriction enzyme PacI to expose ITRs, and transfected into HEK293 cells for adenovirus rescue. The adenovirus was amplified and purified by CsCl banding as described in Engelhardt, J. Methods in Molecular Medicine, Gene Therapy Protocols, 169-184 (P. Robbins ed., Humana Press 1999). Concentrated adenovirus was desalted by using a sterilized Bio-gel column (Bio-Rad) and stored in 1×PBS with 10% glycerol at −80° C.  
      Ob/ob mice are a naturally occurring strain of mice that have a natural deletion of the ob/ob gene, which codes for the cytokine protein Leptin. The resulting deletion of the ob/ob gene has numerous physiological consequences in the ob/ob mouse. These include a desire to consume food in an unrestricted manner with the result that these mice are approximately 100% heavier that the wild type mouse strain C57B1/6. Leptin binds to a cytokine class I receptor, obRb and activates intracellular signalling cascade which curtails feeding. In addition to these mice being obese, they also have a number of other metabolic defects including hyperphagia, reduced thermogenesis, decreased fertility, and inhibition of growth hormone production.  
      Leptin-deficient ob/ob mice have been used as a model system to analyze molecular characteristics of impaired wound healing. The severe wound-healing difficulties observed in ob/ob mice have been explained by the diabetic phenotype of the animals. However, growth factors and cytokines are also central to a normal wound-healing process and thus this strain of mice make a good model system for studying the human diabetic condition particularly as related to the wound repair process.  
      To determine the effect of β-Tectorin on wound repair, ob/ob mice were anesthetized and a 6 mm punch biopsy tool used to make two uniform punch biopsies on the back of the animal. Adenovirus (1×10 10  viral particles/wound or 2×10 10  viral particles/mouse) coding for of β-Tectorin or a control empty adenovirus were separately mixed with pluronic F127 gel 13% in PBS (Sigma Chemical Company Cat # P-2443) at 4° C. and directly applied to the wounded area. The wound was subsequently dressed with a transparent dressing. The mice were monitored on a two-day cycle and the wound area measured as it healed. Wound area measurements were conducted by tracing the outline/margin of the healing wound through transparency film every two days. At the end of the experiment when all animals had healed, either spontaneously or with the aid of β-Tectorin, the transparency with the out line of the wound areas were optically scanned using a commercial scanner (Hewlett-Packard model number 7400C) and the surface area determined using Scion Image software (Scion Corporation, Frederick, Md., USA).  
      Treatment of ob/ob mice with adenovirus coding for β-Tectorin substantially and statistically enhanced the rate and the day of wound closure above no treatment or treatment with an empty vector adenovirus as shown in  FIG. 1 .