Patent Publication Number: US-2023145730-A1

Title: Detection cassette and detection system

Description:
CROSS-REFERENCE TO RELATED APPLICATION 
     This application claims the priority benefits of U.S. provisional application Ser. No. 63/278,111, filed on Nov. 11, 2021, and Taiwanese application serial no. 111132342, filed on Aug. 26, 2022. The entirety of each of the above-mentioned patent applications is hereby incorporated by reference herein and made a part of this specification. 
    
    
     BACKGROUND 
     Technical Field 
     The invention relates to a cassette, and particularly relates to a detection cassette. 
     Description of Related Art 
     In the field of biomedical testing, in order to detect different components in a specimen, such as plasma and blood cells in whole blood, it is usually necessary to perform tests on a plasma detection cassette and a blood cell detection cassette respectively. How to detect different components in the specimen by a single cassette is a research direction of the field. 
     SUMMARY 
     The invention is directed to a detection cassette, which is adapted to detect two components in a specimen (or referred to as a sample). 
     The invention provides a detection cassette adapted to detect a sample, the sample includes a first component and a second component. The detection cassette includes a sample injection hole, a first separation tank, a second separation tank, and a first detection tank and a second detection tank. The first separation tank is communicated with the sample injection hole, where a part of the sample is adapted to enter the first separation tank from the sample injection hole, and the part of the sample is separated into the first component and the second component in the first separation tank. The second separation tank is communicated with the sample injection hole, where another part of the sample is adapted to enter the second separation tank from the sample injection hole, and the another part of the sample is separated into the first component and the second component in the second separation tank. The first detection tank is communicated with the first separation tank, and the first component separated in the first separation tank flows to the first detection tank for detection. The second detection tank is communicated with the second separation tank, and the second component separated in the second separation tank flows to the second detection tank for detection. 
     In an embodiment of the invention, a density of the first component is less than a density of the second component, the first separation tank includes a first bottom portion and a first top portion, and the first component separated in the first separation tank is adapted to be located at the first top portion, and the second component separated in the first separation tank is adapted to be located at the first bottom portion. The second separation tank includes a second bottom portion and a second top portion, the first component separated in the second separation tank is adapted to be located at the second top portion, and the second component separated in the second separation tank is adapted to be located at the second bottom portion. 
     In an embodiment of the invention, the detection cassette further includes a first fluid tank and a first mixing tank. The first fluid tank is adapted to contain a first fluid. The first mixing tank is connected to the first top portion, and the first fluid tank is connected to the first top portion or the first mixing tank. The first mixing tank is located in a first direction of the first top portion and the first fluid tank, and the first component located at the first top portion and the first fluid located in the first fluid tank are adapted to be mixed in the first mixing tank. 
     In an embodiment of the invention, the detection cassette further includes a first quantitative tank and a first bending section. The first quantitative tank is connected to the first mixing tank. The first bending section includes a first section and a second section that are connected in a bending manner, where the first section is connected to the first quantitative tank, the second section is connected to the first detection tank, the first quantitative tank is located in a second direction of the first mixing tank and the first section, and the second direction is opposite to the first direction. 
     In an embodiment of the invention, the detection cassette further includes a quantitative flow channel, a first connection flow channel and an overflow tank. The quantitative flow channel is connected between the sample injection hole and the first separation tank. The first connection flow channel is connected between the first separation tank and the second separation tank. The overflow tank is communicated with the second separation tank. 
     In an embodiment of the invention, the detection cassette further includes a first temporary storage tank, a second temporary storage tank and a second connection flow channel. The first temporary storage tank is communicated between the first separation tank and the first connection flow channel. The first temporary storage tank is located in a first direction of the second separation tank and in a second direction of the first separation tank, and the second direction is opposite to the first direction. The second temporary storage tank is communicated with the overflow tank. The second connection flow channel is communicated with the first temporary storage tank and the second temporary storage tank. The second connection flow channel and the second temporary storage tank are located in the second direction of the first temporary storage tank. 
     In an embodiment of the invention, there is a first necked section between the first bottom portion and the first top portion, and there is a second necked section between the second bottom portion and the second top portion. 
     In an embodiment of the invention, the detection cassette further includes a third temporary storage tank connected to the second top portion, the third temporary storage tank is located in a first direction of the second top portion, and the first component located at the second top portion is adapted to flow into the third temporary storage tank. 
     In an embodiment of the invention, the detection cassette further includes a second fluid tank and a second mixing tank. The second fluid tank is adapted to contain a second fluid and is communicated with the second bottom portion. The second mixing tank is communicated with the second bottom portion. The second bottom portion is located in a second direction of the second fluid tank, the second direction is opposite to the first direction, the second mixing tank is located in the second direction of the second bottom portion, and the second fluid in the second fluid tank is adapted to flow to the second bottom portion, so as to flow to the second mixing tank together with the second component located at the second bottom portion. 
     In an embodiment of the invention, the detection cassette further includes a third fluid tank and a third mixing tank. The third fluid tank is adapted to contain a third fluid. The third mixing tank is communicated with the second mixing tank and the third fluid tank, the third mixing tank is located in a third direction of the third fluid tank and the second mixing tank, and the third direction is orthogonal to the first direction and the second direction, and the third fluid in the third fluid tank and the second component and the second fluid in the second mixing tank are adapted to flow to the third mixing tank. 
     In an embodiment of the invention, the detection cassette further comprises a second quantitative tank and a second bending section. The second quantitative tank is communicated with the third mixing tank. The second bending section includes a third section and a fourth section connected in a bending manner, where the third section is connected to the second quantitative tank, the fourth section is connected to the second detection tank, the second quantitative tank is located in a fourth direction of the third mixing tank and the third section, and the fourth direction is opposite to the third direction. 
     The invention provides a detection system includes a centrifugal platform and a detection cassette. The centrifugal platform has a carrier turntable driven by a driving module. The detection cassette above is placed on the carrier turntable. 
     In an embodiment of the invention, the centrifugal platform further includes a sub turntable arranged on the carrier turntable and driven by another driving module, wherein the detection cassette is placed on the sub turntable. 
     Based on the above description, in the detection cassette of the invention, a part of the sample is adapted to enter the first separation tank from the sample injection hole of the detection cassette, and separated into the first component and the second component in the first separation tank. Another part of the sample is adapted to enter the second separation tank from the sample injection hole, and separated into the first component and the second component in the second separation tank. The first detection tank is communicated with the first separation tank, and the first component separated in the first separation tank flows to the first detection tank for detection. The second detection tank is communicated with the second separation tank, and the second component separated in the second separation tank flows to the second detection tank for detection. Therefore, the invention allows detections of the first component and the second component of one sample be conducted on one detection cassette, which effectively reduces detection cost and time, and increases utilization of the sample. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification. The drawings illustrate embodiments of the invention and, together with the description, serve to explain the principles of the invention. 
         FIG.  1    is a schematic diagram of a detection cassette according to an embodiment of the invention. 
         FIG.  2    is an exploded schematic view of the detection cassette of  FIG.  1   . 
         FIG.  3    is a schematic diagram of  FIG.  2    from another viewing angle. 
         FIG.  4    to  FIG.  14    are schematic diagrams of a flow process of a sample in the detection cassette of  FIG.  1   . 
     
    
    
     DESCRIPTION OF THE EMBODIMENTS 
       FIG.  1    is a schematic diagram of a detection cassette according to an embodiment of the invention.  FIG.  2    is an exploded schematic view of the detection cassette of  FIG.  1   .  FIG.  3    is a schematic diagram of  FIG.  2    from another viewing angle. 
     Referring to  FIG.  1    to  FIG.  3   , the detection cassette  100  of the embodiment is adapted to test a sample  10  ( FIG.  4   ). The detection cassette  100  includes a main body  101  and an upper cover  180 . The upper cover  180  is aligned and assembled with the main body  101  through assembling holes  188 . The main body  101  is provided with a plurality of tanks and flow channels. The upper cover  180  may optionally be a transparent plate, so as to clearly see the flow of the sample  10  in the main body  101 . 
     In the embodiment, the sample  10  may flow into a place between the main body  101  and the upper cover  180  from a sample injection hole  102 , and flow in the main body  101 . In addition, a liquid used to mix with the sample  10  is stored within liquid cartridges  20 ,  22 ,  24  ( FIG.  2   ). 
     As shown in  FIG.  3   , the main body  101  includes liquid cartridge insertion ports  105 ,  106 , and  107 , and the liquid cartridges  20 ,  22 , and  24  may be respectively placed in the liquid cartridge insertion ports  105 ,  106 , and  107 . As shown in  FIG.  2   , the liquid cartridges  20 ,  22 ,  24  respectively include membranes  21 ,  23 ,  25 . The main body  101  further includes three corresponding spikes (not shown) located deep within the liquid cartridge insertion ports  105 ,  106 ,  107 . 
     When the liquid cartridges  20 ,  22  and  24  are placed in the liquid cartridge insertion ports  105 ,  106  and  107 , the three spikes will be next to the membranes  21 ,  23  and  25 , but are not yet contacted to the membranes  21 ,  23  and  25 . When it is necessary to make the liquid in the liquid cartridges  20 ,  22 ,  24  to flow into the main body  101 , the liquid cartridges  20 ,  22 ,  24  may be pushed inward by an external push rod (not shown) to deeper parts of the liquid cartridge insertion ports  105 ,  106 ,  107 , so that the membranes  21 ,  23 ,  25  are punctured by the spikes, and the fluids stored in the liquid cartridges  20 ,  22 ,  24  flow out. As shown in  FIG.  1   , the fluids stored in the liquid cartridges  20 ,  22  and  24  respectively flow into three corresponding tanks of the main body  101  through a first through hole  141 , a second through hole  151  and a third through hole  161 . 
     The detection cassette  100  of the embodiment may be placed on a centrifugal platform (not shown) which includes, for example, a carrier turntable independently driven by a driving module and a sub turntable arranged on the carrier turntable and independently driven by another driving module. The carrier turntable is configured to provide a centrifugal force, and the sub turntable is used to adjust an angle of the detection cassette  100  relative to the carrier turntable, so that the sample  10  in the detection cassette  100  flows under the influence of the centrifugal force for the detection to be conducted. 
     A detailed structure and a rotation direction of the detection cassette  100  and a flow process of the sample  10  in the detection cassette  100  will be described in detail below.  FIG.  4    to  FIG.  14    are schematic diagrams of a flow process of the sample in the detection cassette of  FIG.  1   . 
     Referring to  FIG.  4   , the detection cassette  100  includes a sample injection hole  102  and a quantitative flow channel  104 . The sample  10  may be injected from the sample injection hole  102 , the quantitative flow channel  104  is communicated with the sample injection hole  102 , and the sample  10  may be quantified in the quantitative flow channel  104 . In an embodiment, the quantitative flow channel  104  may only serve as a channel through which the sample  10  flows. As shown in  FIG.  4   , along with the continuous injection of the sample  10 , the sample  10  may move along the quantitative flow channel  104 . 
     As shown in  FIG.  5   , the detection cassette  100  includes a first separation tank  110 , a first temporary storage tank  120 , a first connection flow channel  122 , and a second separation tank  130 . The first separation tank  110  is communicated with the quantitative flow channel  104 , and the second separation tank  130  is communicated with the first separation tank  110 . 
     To be specific, the quantitative flow channel  104  is connected between the sample injection hole  102  and the first separation tank  110 . The first separation tank  110  is connected to the first temporary storage tank  120 , the first temporary storage tank  120  is connected to the first connection flow channel  122 , and the first connection flow channel  122  is connected to the second separation tank  130 . Namely, the first temporary storage tank  120  and the first connection flow channel  122  are located between the first separation tank  110  and the second separation tank  130 , and the first temporary storage tank  120  communicates between the first separation tank  110  and the first connection flow channel  122 . 
     At such phase, a centrifugal direction C is a downward direction in  FIG.  5   . Therefore, as shown in  FIG.  5   , the sample  10  flows downward from the quantitative flow channel  104  to the first separation tank  110 , a part of the sample  10  may stay in the first separation tank  110 , and another part of the sample  10  may overflow from the first separation tank  110  to the second separation tank  130  through the first temporary storage tank  120  and the first connection flow channel  122 . 
     In addition, in the embodiment, the sample  10  includes a first component  12  and a second component  14 , and a density of the first component  12  is smaller than that of the second component  14 . For example, the sample  10  may be whole blood, the first component  12  may be plasma, and the second component  14  may be blood cells. 
     Since the centrifugal direction C is a downward direction in  FIG.  5   , the sample  10  in the first separation tank  110  is separated into the first component  12  and the second component  14  and the sample  10  in the second separation tank  130  is separated into the first component  12  and the second component  14 , under the influence of the centrifugal force. 
     To be specific, the first separation tank  110  includes a first bottom portion  112  and a first top portion  116 . The first component  12  separated in the first separation tank  110  is adapted to be located at the first top portion  116 , and the second component  14  separated in the first separation tank  110  is adapted to be located at the first bottom portion  112 . A first necked section  114  may be optionally provided between the first bottom portion  112  and the first top portion  116 . A width of the first necked section  114  is smaller than that of the first bottom portion  112  and the first top portion  116 , so that the first bottom portion  112  and the first top portion  116  form two tanks. In other embodiments which no necked section  114  is provided between the first bottom portion  112  and the first top portion  116 , the first bottom portion  112  and the first top portion  116  commonly form a single tank. The width of the first bottom portion  112  may be substantially the same as the width of the first top portion  116 , or slightly smaller than the width of the first top portion  116 . 
     Similarly, the second separation tank  130  includes a second bottom portion  132  and a second top portion  136 . A second necked section  134  may be optionally provided between the second bottom portion  132  and the second top portion  136 . The first component  12  separated in the second separation tank  130  is adapted to be located at the second top portion  136 , and the second component  14  separated in the second separation tank  130  is adapted to be located at the second bottom portion  132 . Similarly, in other embodiments, no second necked section  134  is provided between the second bottom portion  132  and the second top portion  136 . 
     Referring to  FIG.  6   , in the embodiment, the detection cassette  100  further includes a first fluid tank  140 . The first fluid tank  140  is adapted to contain a first fluid  30 . As shown in  FIG.  2    and  FIG.  3   , the liquid cartridge  20  ( FIG.  3   ) located in the liquid cartridge insertion port  105  ( FIG.  3   ) may be pushed inward by an external push rod that is not shown, and the membrane  21  ( FIG.  2   ) of the liquid cartridge  20  is punctured by the spike in the main body  101 . As shown in  FIG.  6   , the first fluid  30  stored in the liquid cartridge  20  is moved in the centrifugal direction C by the centrifugal force to flow into the first fluid tank  140 . In the embodiment, the first fluid  30  may be a diluent, but the type of the first fluid  30  is not limited thereto. 
     Moreover, referring to  FIG.  7   , the detection cassette  100  further includes a first mixing tank  142 . The first mixing tank  142  is connected to the first top portion  116 , and the first fluid tank  140  is connected to the first top portion  116  or the first mixing tank  142 . 
     When the detection cassette  100  is rotated as shown in  FIG.  7   , the centrifugal direction C is in a first direction D 1 . The first mixing tank  142  is located in the first direction D 1  of the first top portion  116  and the first fluid tank  140 , i.e. at a lower position of  FIG.  7   . Namely, when the centrifugal direction C is in the first direction D 1 , the first mixing tank  142  is further away from a rotation center (not shown) than the first top portion  116  and the first fluid tank  140 . Therefore, the first component  12  located at the first top portion  116  and the first fluid  30  located in the first fluid tank  140  flow into the first mixing tank  142 , and are mixed in the first mixing tank  142  to form a first mixed solution  32 . 
     On the other hand, the detection cassette  100  further includes a third temporary storage tank  124  communicated with the second top portion  136 . The third temporary storage tank  124  is located in the first direction D 1  of the second top portion  136 , so that the first component  12  located at the second top portion  136  flows into the third temporary storage tank  124  and is separated from the second component  14  remained at the second bottom portion  132 . 
     It should be noted that, generally, about 50% to 60% of the whole blood is plasma, and about 40% to 50% is blood cells. Since the second component  14  (blood cells) is to be tested after it is separated from the sample  10  in the second separation tank  130 , in the embodiment, a volume of the second bottom portion  132  may be optionally smaller than a volume of the second top portion  136 . Preferably, the volume of the second bottom portion  132  is less than 35% of the second separation tank  130 , which may be, for example, 30%. Such design may ensure that the second bottom portion  132  contains only the second component  14 . In this way, when the detection cassette  100  is rotated from the position shown in  FIG.  6    to the position shown in  FIG.  7   , the part of the sample  10  at the second top portion  136  flows to the third temporary storage tank  124 , and only the part of the sample  10  at the second bottom portion  132  is remained in the second separation tank  130 , so as to prevent the first component  12  from remaining in the second separation tank  130  to interfere subsequent detection results of the second component  14 . 
     In addition, since the third temporary storage tank  124  is communicated with the overflow tank  128 , when the detection cassette  100  is rotated as shown in  FIG.  8   , the overflow tank  128  is located in the centrifugal direction C of the third temporary storage tank  124 , which is at a lower position of  FIG.  8   , so that the first component  12  in the third temporary storage tank  124  may flow to the overflow tank  128  under the centrifugal force. At this time, in addition to collection of the excess fluid, an optical detection may be conducted at the overflow tank  128  to detect optical background parameters of the overflowing fluid (the first component  12 ) before reacting with a reagent. 
     The detection cassette  100  further includes a second fluid tank  150 . Referring to  FIG.  2   ,  FIG.  3    and  FIG.  8   , the liquid cartridge  22  ( FIG.  3   ) located in the liquid cartridge insertion port  106  ( FIG.  3   ) may be pushed inward by the external push rod, and the membrane  23  ( FIG.  2   ) of the liquid cartridge  22  is punctured by the spike in the main body  101 . As shown in  FIG.  8   , the second fluid  40  stored in the liquid cartridge  22  is moved in the centrifugal direction C by the centrifugal force to flow into the second fluid tank  150 . The second fluid  40  may be a reagent solution for blood cells, but the type of the second fluid  40  is not limited thereto. 
     Then, referring to  FIG.  9   , the detection cassette  100  further includes a first quantitative tank  144 , a first bending section  145  and first detection tanks  148 ,  149 . In the embodiment, the number of the first quantitative tank  144 , the first bending section  145 , the first detection tank  148 , and the first detection tank  149  are each plural. Certainly, the detection cassette  100  may alternatively have only a single first quantitative tank  144 , a single first bending section  145 , a single first detection tank  148  and a single first detection tank  149 . The number of these elements is not limited thereto. 
     The first quantitative tank  144  is connected to the first mixing tank  142 . The first bending section  145  includes a first section  146  and a second section  147  connected in a bending manner. The first section  146  is connected to the first quantitative tank  144 , and the second section  147  is connected to the first detection tanks  148  and  149 . As shown in  FIG.  9   , when the centrifugal direction C of the detection cassette  100  is in a second direction D 2 , the first quantitative tank  144  is in the second direction D 2  of the first mixing tank  142 , i.e., at a lower position of  FIG.  9   . The second direction D 2  is the opposite direction to the first direction D 1 . Therefore, the first mixed solution  32  located in the first mixing tank  142  flows into the first quantitative tank  144 . 
     In addition, in the embodiment, the first quantitative tank  144  is located in the second direction D 2  of the first segment  146 . Therefore, the first mixed solution  32  does not flow to the first section  146  at this phase, but flows to other first quantitative tanks  144  connected in the centrifugal direction C (lower position of  FIG.  9   ) in sequence, so that a plurality of quantified first mixed solution  32  may be obtained for subsequent tests. 
     On the other hand, the detection cassette  100  further includes a second temporary storage tank  126  and a second connection flow channel  182 . The second connection flow channel  182  is connected to the first temporary storage tank  120  and the second temporary storage tank  126 , and the second temporary storage tank  126  is connected to the overflow tank  128 . In the embodiment, the second connection flow channel  182  is located on the upper cover  180 , and such design may save a space on the main body  101 . 
     As shown in  FIG.  9   , the centrifugal direction C of the detection cassette  100  is in the second direction D 2 . The first temporary storage tank  120  is located in the second direction D 2  of the first separation tank  110 , and the second connection flow channel  182  and the second temporary storage tank  126  are located in the second direction D 2  (a lower position of  FIG.  9   ) of the first temporary storage tank  120 . Namely, the first temporary storage tank  120  is farther from the rotation center (not shown) than the first separation tank  110 , and the second connection flow channel  182  and the second temporary storage tank  126  are farther from the rotation center than the first temporary storage tank  120 . Therefore, the second component  14  from the first bottom portion  112  of the first separation tank  110  may flow to the second temporary storage tank  126  along the second connection flow channel  182 , and then flow to the overflow tank  128 . At this time, the overflow tank  128  may be used to collect excess fluid. In this way, the first separation tank  110  and the second separation tank  130  may share the overflow tank  128 , thereby saving space on the detection cassette. 
     Moreover, in the embodiment, the detection cassette  100  further includes a second mixing tank  152 . The second fluid tank  150  is communicated with the second bottom portion  132 , and the second mixing tank  152  is communicated with the second bottom portion  132 . In  FIG.  9   , the second bottom portion  132  is located in the second direction D 2  (a lower position in  FIG.  9   ) of the second fluid tank  150 , and the second mixing tank  152  is located in the second direction D 2  (a lower position in  FIG.  9   ) of the second bottom portion  132 . Therefore, the second fluid  40  in the second fluid tank  150  is adapted to flow to the second bottom portion  132 , so as to flow to the second mixing tank  152  together with the second component  14  located at the second bottom portion  132  to form a second mixed solution  42 . 
     Then, referring to  FIG.  10   , in the embodiment, the first detection tanks  148  and  149  may be filled with a reagent or medicament, preferably a dry reagent or medicament. The detection cassette  100  is rotated so that the first bending section  145  is located in the centrifugal direction C of the first quantitative tank  144  (a lower position of  FIG.  10   ). Therefore, the first mixed solution  32  located in the first quantitative tank  144  flows from the first bending section  145  to the first detection tank  148 . The first mixed solution  32  may be mixed with the reagent or medicament in the first detection tank  148 . 
     On the other hand, in the embodiment, the detection cassette  100  further includes a third fluid tank  160 . The third fluid tank  160  is adapted to contain a third fluid  50 . Referring to  FIG.  2   ,  FIG.  3    and  FIG.  10   , the liquid cartridge  24  ( FIG.  3   ) located in the liquid cartridge insertion port  107  ( FIG.  3   ) may be pushed inward by the external push rod, and the membrane  25  ( FIG.  2   ) of the liquid cartridge  24  is punctured by the spike in the main body  101 . As shown in  FIG.  10   , the third fluid  50  stored in the liquid cartridge  24  is moved in the centrifugal direction C by the centrifugal force and flows into the third fluid tank  160 . The third fluid  50  may be another reagent liquid for blood cells, but the type of the third fluid  50  is not limited thereto. 
     Referring to  FIG.  11   , the detection cassette  100  is rotated. When the centrifugal direction C of the detection cassette  100  is in a third direction D 3  which is substantially orthogonal to the first direction D 1  and the second direction D 2 , the first mixed solution  32  located in the first detection tank  148  flows to the other first detection tank  149  to fully mix the first mixed solution  32  with the reagent or medicament in the first detection tanks  148  and  149 . 
     On the other hand, the detection cassette  100  further includes a third mixing tank  162 . The third mixing tank  162  is communicated with the second mixing tank  152  and the third fluid tank  160 . The third mixing tank  162  is located in the third direction D 3  of the third fluid tank  160  and the second mixing tank  152 . Therefore, the third fluid  50  located in the third fluid tank  160  and the second component  14  and the second fluid  40  located in the second mixing tank  152  flow to the third mixing tank  162  to form a third mixed solution  52 . 
     Then, referring to  FIG.  12   , the detection cassette  100  is rotated. When the centrifugal direction C of the detection cassette  100  is in a fourth direction D 4 , the first mixed solution  32  located in the first detection tank  149  flows back to the first detection tank  148 , so that the first mixed solution  32  can be mixed with the reagent or medicament between the two first detection tanks  148  and  149 . 
     On the other hand, in the embodiment, the detection cassette  100  further includes one or a plurality of second quantitative tanks  170 , second bending sections  172 , second detection tanks  178 , and second detection tanks  179 . The second quantitative tank  170  is communicated with the third mixing tank  162 . The second bending section  172  includes a third section  174  and a fourth section  176  connected in a bending manner. The third section  174  is connected to the second quantitative tank  170 , and the fourth section  176  is connected to the second detection tanks  178  and  179 . 
     When the centrifugal direction C of the detection cassette  100  is in the fourth direction D 4 , the second quantitative tank  170  is located in the fourth direction D 4  of the third mixing tank  162  and the third section  174 . The fourth direction D 4  is opposite to the third direction D 3 . Therefore, the third mixed solution  52  located in the third mixing tank  162  flows to the second quantitative tank  170 . 
     Referring to  FIG.  13   , the detection cassette  100  is rotated, and the first mixed solution  32  is mixed with the reagent or medicament in the first detection tanks  148  and  149 . On the other hand, the third mixed solution  52  located in the second quantitative tank  170  flows from the second bending section  172  to the second detection tank  178 . Similarly, the second detection tank  178  may be filled with a medicament or reagent, preferably a dry medicament or reagent, used for detecting the second component  14 , but the invention is not limited thereto. 
     Then, referring to  FIG.  14   , the detection cassette  100  is rotated, and the first mixed solution  32  located in the first detection tank  149  flows to the first detection tank  148  to be mixed with the reagent or medicament of the first component  12  again. At the same time, the third mixed solution  52  located in the second detection tank  178  flows to the second detection tank  179  to be mixed with the reagent or medicament of the second component  14 . 
     Referring back to  FIG.  1   , in the embodiment, the upper cover  180  further includes gas escape holes  184  and  186 . The gas escape holes  184  are communicated with the first detection tanks  148  and  149 , and a gas in the first detection tanks  148  and  149  ( FIG.  14   ) may be discharged from the gas escape holes  184 . The gas escape holes  186  are communicated with the second detection tanks  178  and  179 , and the gas in the second detection tanks  178  and  179  ( FIG.  14   ) may be discharged from the gas escape holes  186 . As a result, the first mixed solution  32  in the first detection tanks  148  and  149  and the third mixed solution  52  in the second detection tanks  178  and  179  can flow smoothly. 
     The steps shown in  FIG.  13    and  FIG.  14    can be repeated several times to ensure that the first mixed solution  32  and the reagent or medicament are completely mixed. Once mixing of the third mixed solution  52  and the reagent or medicament is completed, detection of the first component  12  may be performed in the first detection tanks  148  and  149 , and detection of the second component  14  may be performed in the second detection tanks  178  and  179 . The detection is, for example, optical detection or other detections. For example, an external optical device (not shown) may be used to detect the mixtures in the first detection tanks  148 ,  149  and the second detection tanks  178 ,  179  through the transparent upper cover  180 . Alternatively, the transparent upper cover  180  may be removed and then the quantified mixtures may be taken out from the detection cassette to perform other detections. 
     As describe above, after the sample  10  is injected into the detection cassette  100  of the embodiment, the sample  10  can be separated into the first component  12  and the second component  14  by centrifugal force by rotating the detection cassette  100  to different directions. After the first component  12  and the second component  14  are mixed with the corresponding fluids, the mixtures flow to the first detection tanks  148 ,  149  and the second detection tanks  178 ,  179 . Detections of the first component  12  and the second component  14  can be respectively performed in the first detection tanks  148 ,  149  and the second detection tanks  178 ,  179 . Therefore, the first component  12  and the second component  14  may be detected in one single detection cassette  100 , which greatly shortens the required detection time, sample amount, and amount of the detection cassettes. 
     In summary, in the detection cassette of the invention, a part of the sample is adapted to enter the first separation tank from the sample injection hole of the detection cassette, and separated into the first component and the second component in the first separation tank. Another part of the sample is adapted to enter the second separation tank from the sample injection hole, and separated into the first component and the second component in the second separation tank. The first detection tank is communicated with the first separation tank, and the first component separated in the first separation tank flows to the first detection tank for detection. The second detection tank is communicated with the second separation tank, and the second component separated in the second separation tank flows to the second detection tank for detection. Therefore, the invention allows detections of the first component and the second component of one sample be conducted on one detection cassette, which effectively reduces detection cost and time, and increases utilization of the sample.