Patent Publication Number: US-2003229020-A1

Title: Compositions for the treatment and diagnosis of breast cancer and methods for their use

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS  
     [0001] This application is a continuation-in-part of U.S. application Ser. No. 09/222,575, filed Dec. 28, 1998. 
    
    
     
       TECHNICAL FIELD  
       [0002] The present invention relates generally to compositions and methods for the treatment of breast cancer. The invention is more particularly related to polypeptides comprising at least a portion of a protein that is preferentially expressed in breast tumor tissue and to polynucleotides encoding such polypeptides. Such polypeptides and polynucleotides may be used in vaccines and pharmaceutical compositions for treatment of breast cancer.  
       BACKGROUND OF THE INVENTION  
       [0003] Breast cancer is a significant health problem for women in the United States and throughout the world. Although advances have been made in detection and treatment of the disease, breast cancer remains the second leading cause of cancer-related deaths in women, affecting more than 180,000 women in the United States each year. For women in North America, the life-time odds of getting breast cancer are one in eight.  
       [0004] No vaccine or other universally successful method for the prevention or treatment of breast cancer is currently available. Management of the disease currently relies on a combination of early diagnosis (through routine breast screening procedures) and aggressive treatment, which may include one or more of a variety of treatments such as surgery, radiotherapy, chemotherapy and hormone therapy. The course of treatment for a particular breast cancer is often selected based on a variety of prognostic parameters, including an analysis of specific tumor markers. See, e.g., Porter-Jordan and Lippman,  Breast Cancer  8:73-100 (1994). However, the use of established markers often leads to a result that is difficult to interpret, and the high mortality observed in breast cancer patients indicates that improvements are needed in the treatment, diagnosis and prevention of the disease.  
       [0005] Accordingly, there is a need in the art for improved methods for the treatment and diagnosis of breast cancer. The present invention fulfills these needs and further provides other related advantages.  
       SUMMARY OF THE INVENTION  
       [0006] The present invention provides compounds and methods for the treatment and diagnosis of breast cancer. In one aspect, isolated polypeptides are provided comprising at least an immunogenic portion of a breast tumor antigen or a variant thereof, wherein the antigen comprises an amino acid sequence encoded by a polynucleotide having a sequence selected from the group consisting of: (a) nucleotide sequences recited in SEQ ID NO: 1-61, 63-175, 178 and 180; (b) complements of said nucleotide sequences; and (c) sequences that hybridize to a sequence of (a) or (b) under moderately stringent conditions. In specific embodiments, the inventive polypeptides comprise an amino acid sequence selected from the group consisting of SEQ ID NO: 62, 176, 179 and 181.  
       [0007] In related aspects, isolated polynucleotides encoding the above polypeptides are provided. In specific embodiments, such polynucleotides comprise a sequence selected from the group consisting of sequences provided in SEQ ID NO: 1-61, 63-175, 178 and 180. The present invention further provides expression vectors comprising the above polynucleotides, together with host cells transformed or transfected with such expression vectors. In preferred embodiments, the host cells are selected from the group consisting of  E coli , yeast and mammalian cells.  
       [0008] In another aspect, the present invention provides fusion proteins comprising a first and a second inventive polypeptide or, alternatively, an inventive polypeptide and a known breast tumor antigen.  
       [0009] The present invention also provides pharmaceutical compositions comprising at least one of the above polypeptides, or a polynucleotide encoding such a polypeptide, and a physiologically acceptable carrier, together with vaccines comprising at least one such polypeptide or polynucleotide in combination with a non-specific immune response enhancer. Pharmaceutical compositions and vaccines comprising one or more of the above fusion proteins are also provided.  
       [0010] In yet another aspect, methods are provided for inhibiting the development of breast cancer in a patient, comprising administering an effective amount of at least one of the above pharmaceutical compositions and/or vaccines.  
       [0011] The polypeptides disclosed herein may be usefully employed in the diagnosis and monitoring of breast cancer. In one aspect of the present invention, methods are provided for detecting breast cancer in a patient, comprising: (a) contacting a biological sample obtained from a patient with a binding agent that is capable of binding to one of the above polypeptides; and (b) detecting in the sample a protein or polypeptide that binds to the binding agent. In preferred embodiments, the binding agent is an antibody, most preferably a monoclonal antibody.  
       [0012] In related aspects, methods are provided for monitoring the progression of breast cancer in a patient, comprising: (a) contacting a biological sample obtained from a patient with a binding agent that is capable of binding to one of the above polypeptides; (b) determining in the sample an amount of a protein or polypeptide that binds to the binding agent; (c) repeating steps (a) and (b); and comparing the amounts of polypeptide detected in steps (b) and (c).  
       [0013] Within related aspects, the present invention provides antibodies, preferably monoclonal antibodies, that bind to the inventive polypeptides, as well as diagnostic kits comprising such antibodies, and methods of using such antibodies to inhibit the development of breast cancer.  
       [0014] The present invention further provides methods for detecting breast cancer comprising: (a) obtaining a biological sample from a patient; (b) contacting the sample with a first and a second oligonucleotide primer in a polymerase chain reaction, at least one of the oligonucleotide primers being specific for a polynucleotide that encodes one of the above polypeptides; and (c) detecting in the sample a DNA sequence that amplifies in the presence of the first and second oligonucleotide primers. In a preferred embodiment, at least one of the oligonucleotide primers comprises at least about 10 contiguous nucleotides of a polynucleotide comprising a sequence selected from the group consisting of SEQ ID NO: 1-61, 63-175, 178 and 180.  
       [0015] In a further aspect, the present invention provides a method for detecting breast cancer in a patient comprising: (a) obtaining a biological sample from the patient; (b) contacting the sample with an oligonucleotide probe specific for a polynucleotide that encodes one of the above polypeptides; and (c) detecting in the sample a DNA sequence that hybridizes to the oligonucleotide probe. Preferably, the oligonucleotide probe comprises at least about 15 contiguous nucleotides of a polynucleotide comprising a sequence selected from the group consisting of SEQ ID NO: 1-61, 63-175, 178 and 180.  
       [0016] In related aspects, diagnostic kits comprising the above oligonucleotide probes or primers are provided.  
       [0017] These and other aspects of the present invention will become apparent upon reference to the following detailed description. All references disclosed herein are hereby incorporated by reference in their entirety as if each was incorporated individually. 
     
    
    
     BRIEF DESCRIPTION OF THE DRAWING AND SEQUENCE IDENTIFIERS  
     [0018]FIG. 1 shows the results of a Northern blot of the clone SYN18C6 (SEQ ID NO: 40).  
     [0019] SEQ ID NO: 1 is the determined cDNA sequence of JBT2.  
     [0020] SEQ ID NO: 2 is the determined cDNA sequence of JBT6.  
     [0021] SEQ ID NO: 3 is the determined cDNA sequence of JBT7.  
     [0022] SEQ ID NO: 4 is the determined cDNA sequence of JBT10.  
     [0023] SEQ ID NO: 5 is the determined cDNA sequence of JBT13.  
     [0024] SEQ ID NO: 6 is the determined cDNA sequence of JBT14.  
     [0025] SEQ ID NO: 7 is the determined cDNA sequence of JBT15.  
     [0026] SEQ ID NO: 8 is the determined cDNA sequence of JBT16.  
     [0027] SEQ ID NO: 9 is the determined cDNA sequence of JBT17.  
     [0028] SEQ ID NO: 10 is the determined cDNA sequence of JBT22.  
     [0029] SEQ ID NO: 11 is the determined cDNA sequence of JBT25.  
     [0030] SEQ ID NO: 12 is the determined cDNA sequence of JBT28.  
     [0031] SEQ ID NO: 13 is the determined cDNA sequence of JBT32.  
     [0032] SEQ ID NO: 14 is the determined cDNA sequence of JBT33.  
     [0033] SEQ ID NO: 15 is the determined cDNA sequence of JBT34.  
     [0034] SEQ ID NO: 16 is the determined cDNA sequence of JBT36.  
     [0035] SEQ ID NO: 17 is the determined cDNA sequence of JBT37.  
     [0036] SEQ ID NO: 18 is the determined cDNA sequence of JBT51.  
     [0037] SEQ ID NO: 19 is the determined cDNA sequence of JBTT1.  
     [0038] SEQ ID NO: 20 is the determined cDNA sequence of JBTT7.  
     [0039] SEQ ID NO: 21 is the determined cDNA sequence of JBTT11.  
     [0040] SEQ ID NO: 22 is the determined cDNA sequence of JBTT14.  
     [0041] SEQ ID NO: 23 is the determined cDNA sequence of JBTT18.  
     [0042] SEQ ID NO: 24 is the determined cDNA sequence of JBTT19.  
     [0043] SEQ ID NO: 25 is the determined cDNA sequence of JBTT20.  
     [0044] SEQ ID NO: 26 is the determined cDNA sequence of JBTT21.  
     [0045] SEQ ID NO: 27 is the determined cDNA sequence of JBTT22.  
     [0046] SEQ ID NO: 28 is the determined cDNA sequence of JBTT28.  
     [0047] SEQ ID NO: 29 is the determined cDNA sequence of JBTT29.  
     [0048] SEQ ID NO: 30 is the determined cDNA sequence of JBTT33.  
     [0049] SEQ ID NO: 31 is the determined cDNA sequence of JBTT37.  
     [0050] SEQ ID NO: 32 is the determined cDNA sequence of JBTT38.  
     [0051] SEQ ID NO: 33 is the determined cDNA sequence of JBTT47.  
     [0052] SEQ ID NO: 34 is the determined cDNA sequence of JBTT48.  
     [0053] SEQ ID NO: 35 is the determined cDNA sequence of JBTT50.  
     [0054] SEQ ID NO: 36 is the determined cDNA sequence of JBTT51.  
     [0055] SEQ ID NO: 37 is the determined cDNA sequence of JBTT52.  
     [0056] SEQ ID NO: 38 is the determined cDNA sequence of JBTT54.  
     [0057] SEQ ID NO: 39 is the determined cDNA sequence of SYN17F4.  
     [0058] SEQ ID NO: 40 is the determined cDNA sequence of SYN18C6.  
     [0059] SEQ ID NO: 41 is the determined cDNA sequence of SYN19A2.  
     [0060] SEQ ID NO: 42 is the determined cDNA sequence of SYN19C8.  
     [0061] SEQ ID NO: 43 is the determined cDNA sequence of SYN20A12.  
     [0062] SEQ ID NO: 44 is the determined cDNA sequence of SYN20G6.  
     [0063] SEQ ID NO: 45 is the determined cDNA sequence of SYN20G6-2.  
     [0064] SEQ ID NO: 46 is the determined cDNA sequence of SYN21B9.  
     [0065] SEQ ID NO: 47 is the determined cDNA sequence of SYN21B9-2.  
     [0066] SEQ ID NO: 48 is the determined cDNA sequence of SYN21C10.  
     [0067] SEQ ID NO: 49 is the determined cDNA sequence of SYN21G10.  
     [0068] SEQ ID NO: 50 is the determined cDNA sequence of SYN21G10-2.  
     [0069] SEQ ID NO: 51 is the determined cDNA sequence of SYN21G11.  
     [0070] SEQ ID NO: 52 is the determined cDNA sequence of SYN21G11-2.  
     [0071] SEQ ID NO: 53 is the determined cDNA sequence of SYN21H8.  
     [0072] SEQ ID NO: 54 is the determined cDNA sequence of SYN22A10.  
     [0073] SEQ ID NO: 55 is the determined cDNA sequence of SYN22A10-2.  
     [0074] SEQ ID NO: 56 is the determined cDNA sequence of SYN22A12.  
     [0075] SEQ ID NO: 57 is the determined cDNA sequence of SYN22A2.  
     [0076] SEQ ID NO: 58 is the determined cDNA sequence of SYN22B4.  
     [0077] SEQ ID NO: 59 is the determined cDNA sequence of SYN22C2.  
     [0078] SEQ ID NO: 60 is the determined cDNA sequence of SYN22E10.  
     [0079] SEQ ID NO: 61 is the determined cDNA sequence of SYN22F2.  
     [0080] SEQ ID NO: 62 is a predicted amino acid sequence for SYN18C6.  
     [0081] SEQ ID NO: 63 is the determined cDNA sequence of B723P.  
     [0082] SEQ ID NO: 64 is the determined cDNA sequence for B724P.  
     [0083] SEQ ID NO: 65 is the determined cDNA sequence of B770P.  
     [0084] SEQ ID NO: 66 is the determined cDNA sequence of B716P.  
     [0085] SEQ ID NO: 67 is the determined cDNA sequence of B725P.  
     [0086] SEQ ID NO: 68 is the determined cDNA sequence of B717P.  
     [0087] SEQ ID NO: 69 is the determined cDNA sequence of B771P.  
     [0088] SEQ ID NO: 70 is the determined cDNA sequence of B722P.  
     [0089] SEQ ID NO: 71 is the determined cDNA sequence of B726P.  
     [0090] SEQ ID NO: 72 is the determined cDNA sequence of B727P.  
     [0091] SEQ ID NO: 73 is the determined cDNA sequence of B728P.  
     [0092] SEQ ID NO: 74-87 are the determined cDNA sequences of isolated clones which show homology to known sequences.  
     [0093] SEQ ID NO: 88 is the determined cDNA sequence of 13053.  
     [0094] SEQ ID NO: 89 is the determined cDNA sequence of 13057.  
     [0095] SEQ ID NO: 90 is the determined cDNA sequence of 13059.  
     [0096] SEQ ID NO: 91 is the determined cDNA sequence of 13065.  
     [0097] SEQ ID NO: 92 is the determined cDNA sequence of 13067.  
     [0098] SEQ ID NO: 93 is the determined cDNA sequence of 13068.  
     [0099] SEQ ID NO: 94 is the determined cDNA sequence of 13071.  
     [0100] SEQ ID NO: 95 is the determined cDNA sequence of 13072.  
     [0101] SEQ ID NO: 96 is the determined cDNA sequence of 13073.  
     [0102] SEQ ID NO: 97 is the determined cDNA sequence of 13075.  
     [0103] SEQ ID NO: 98 is the determined cDNA sequence of 13078.  
     [0104] SEQ ID NO: 99 is the determined cDNA sequence of 13079.  
     [0105] SEQ ID NO: 100 is the determined cDNA sequence of 13081.  
     [0106] SEQ ID NO: 101 is the determined cDNA sequence of 13082.  
     [0107] SEQ ID NO: 102 is the determined cDNA sequence of 13092.  
     [0108] SEQ ID NO: 103 is the determined cDNA sequence of 13097.  
     [0109] SEQ ID NO: 104 is the determined cDNA sequence of 13101.  
     [0110] SEQ ID NO: 105 is the determined cDNA sequence of 13102.  
     [0111] SEQ ID NO: 106 is the determined cDNA sequence of 13119.  
     [0112] SEQ ID NO: 107 is the determined cDNA sequence of 13131.  
     [0113] SEQ ID NO: 108 is the determined cDNA sequence of 13133.  
     [0114] SEQ ID NO: 109 is the determined cDNA sequence of 13135.  
     [0115] SEQ ID NO: 110 is the determined cDNA sequence of 13139.  
     [0116] SEQ ID NO: 111 is the determined cDNA sequence of 13140.  
     [0117] SEQ ID NO: 112 is the determined cDNA sequence of 13146.  
     [0118] SEQ ID NO: 113 is the determined cDNA sequence of 13147.  
     [0119] SEQ ID NO: 114 is the determined cDNA sequence of 13148.  
     [0120] SEQ ID NO: 115 is the determined cDNA sequence of 13149.  
     [0121] SEQ ID NO: 116 is the determined cDNA sequence of 13151.  
     [0122] SEQ ID NO: 117 is the determined cDNA sequence of 13051  
     [0123] SEQ ID NO: 118 is the determined cDNA sequence of 13052  
     [0124] SEQ ID NO: 119 is the determined cDNA sequence of 13055  
     [0125] SEQ ID NO: 120 is the determined cDNA sequence of 13058  
     [0126] SEQ ID NO: 121 is the determined cDNA sequence of 13062  
     [0127] SEQ ID NO: 122 is the determined cDNA sequence of 13064  
     [0128] SEQ ID NO: 123 is the determined cDNA sequence of 13080  
     [0129] SEQ ID NO: 124 is the determined cDNA sequence of 13093  
     [0130] SEQ ID NO: 125 is the determined cDNA sequence of 13094  
     [0131] SEQ ID NO: 126 is the determined cDNA sequence of 13095  
     [0132] SEQ ID NO: 127 is the determined cDNA sequence of 13096  
     [0133] SEQ ID NO: 128 is the determined cDNA sequence of 13099  
     [0134] SEQ ID NO: 129 is the determined cDNA sequence of 13100  
     [0135] SEQ ID NO: 130 is the determined cDNA sequence of 13103  
     [0136] SEQ ID NO: 131 is the determined cDNA sequence of 13106  
     [0137] SEQ ID NO: 132 is the determined cDNA sequence of 13107  
     [0138] SEQ ID NO: 133 is the determined cDNA sequence of 13108  
     [0139] SEQ ID NO: 134 is the determined cDNA sequence of 13121  
     [0140] SEQ ID NO: 135 is the determined cDNA sequence of 13126  
     [0141] SEQ ID NO: 136 is the determined cDNA sequence of 13129  
     [0142] SEQ ID NO: 137 is the determined cDNA sequence of 13130  
     [0143] SEQ ID NO: 138 is the determined cDNA sequence of 13134  
     [0144] SEQ ID NO: 139 is the determined cDNA sequence of 13141  
     [0145] SEQ ID NO: 140 is the determined cDNA sequence of 13142  
     [0146] SEQ ID NO: 141 is the determined cDNA sequence of 14376  
     [0147] SEQ ID NO: 142 is the determined cDNA sequence of 14377  
     [0148] SEQ ID NO: 143 is the determined cDNA sequence of 14383  
     [0149] SEQ ID NO: 144 is the determined cDNA sequence of 14384  
     [0150] SEQ ID NO: 145 is the determined cDNA sequence of 14387  
     [0151] SEQ ID NO: 146 is the determined cDNA sequence of 14392  
     [0152] SEQ ID NO: 147 is the determined cDNA sequence of 14394  
     [0153] SEQ ID NO: 148 is the determined cDNA sequence of 14398  
     [0154] SEQ ID NO: 149 is the determined cDNA sequence of 14401  
     [0155] SEQ ID NO: 150 is the determined cDNA sequence of 14402  
     [0156] SEQ ID NO: 151 is the determined cDNA sequence of 14405  
     [0157] SEQ ID NO: 152 is the determined cDNA sequence of 14409  
     [0158] SEQ ID NO: 153 is the determined cDNA sequence of 14412  
     [0159] SEQ ID NO: 154 is the determined cDNA sequence of 14414  
     [0160] SEQ ID NO: 155 is the determined cDNA sequence of 14415  
     [0161] SEQ ID NO: 156 is the determined cDNA sequence of 14416  
     [0162] SEQ ID NO: 157 is the determined cDNA sequence of 14419  
     [0163] SEQ ID NO: 158 is the determined cDNA sequence of 14426  
     [0164] SEQ ID NO: 159 is the determined cDNA sequence of 14427  
     [0165] SEQ ID NO: 160 is the determined cDNA sequence of 14375  
     [0166] SEQ ID NO: 161 is the determined cDNA sequence of 14378  
     [0167] SEQ ID NO: 162 is the determined cDNA sequence of 14379  
     [0168] SEQ ID NO: 163 is the determined cDNA sequence of 14380  
     [0169] SEQ ID NO: 164 is the determined cDNA sequence of 14381  
     [0170] SEQ ID NO: 165 is the determined cDNA sequence of 14382  
     [0171] SEQ ID NO: 166 is the determined cDNA sequence of 14388  
     [0172] SEQ ID NO: 167 is the determined cDNA sequence of 14399  
     [0173] SEQ ID NO: 168 is the determined cDNA sequence of 14406  
     [0174] SEQ ID NO: 169 is the determined cDNA sequence of 14407  
     [0175] SEQ ID NO: 170 is the determined cDNA sequence of 14408  
     [0176] SEQ ID NO: 171 is the determined cDNA sequence of 14417  
     [0177] SEQ ID NO: 172 is the determined cDNA sequence of 14418  
     [0178] SEQ ID NO: 173 is the determined cDNA sequence of 14423  
     [0179] SEQ ID NO: 174 is the determined cDNA sequence of 14424  
     [0180] SEQ ID NO: 175 is the determined cDNA sequence of B726P-20  
     [0181] SEQ ID NO: 176 is the predicted amino acid sequence of B726P-20  
     [0182] SEQ ID NO: 177 is a PCR primer  
     [0183] SEQ ID NO: 178 is the determined cDNA sequence of B726P-74  
     [0184] SEQ ID NO: 179 is the predicted amino acid sequence of B726P-74  
     [0185] SEQ ID NO: 180 is the determined cDNA sequence of B726P-79  
     [0186] SEQ ID NO: 181 is the predicted amino acid sequence of B726P-79  
    
    
     DETAILED DESCRIPTION OF THE INVENTION  
     [0187] As noted above, the present invention is generally directed to compositions and methods for the treatment and diagnosis of breast cancer. The inventive compositions are generally isolated polypeptides that comprise at least a portion of a breast tumor antigen. Also included within the present invention are molecules (such as an antibody or fragment thereof) that bind to the inventive polypeptides. Such molecules are referred to herein as “binding agents.” In particular, the subject invention discloses polypeptides comprising at least a portion of a human breast tumor antigen, or a variant thereof, wherein the breast tumor antigen includes an amino acid sequence encoded by a polynucleotide including a sequence selected from the group consisting of: nucleotide sequences recited in SEQ ID NO: 1-61, 63-175, 178 and 180, the complements of said nucleotide sequences, and variants thereof. As used herein, the term “polypeptide” encompasses amino acid chains of any length, including full length proteins, wherein the amino acid residues are linked by covalent peptide bonds. Thus, a polypeptide comprising a portion of one of the above breast antigens may consist entirely of the portion, or the portion may be present within a larger polypeptide that contains additional sequences. The additional sequences may be derived from the native protein or may be heterologous, and such sequences may be immunoreactive and/or antigenic.  
     [0188] As used herein, an “immunogenic portion” of a human breast tumor antigen is a portion that is capable of eliciting an immune response in a patient inflicted with breast cancer and as such binds to antibodies present within sera from a breast cancer patient. Such immunogenic portions generally comprise at least about 5 amino acid residues, more preferably at least about 10, and most preferably at least about 20 amino acid residues. Immunogenic portions of the proteins described herein may be identified in antibody binding assays. Such assays may generally be performed using any of a variety of means known to those of ordinary skill in the art, as described, for example, in Harlow and Lane,  Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1988. For example, a polypeptide may be immobilized on a solid support (as described below) and contacted with patient sera to allow binding of antibodies within the sera to the immobilized polypeptide. Unbound sera may then be removed and bound antibodies detected using, for example,  125 I-labeled Protein A. Alternatively, a polypeptide may be used to generate monoclonal and polyclonal antibodies for use in detection of the polypeptide in blood or other fluids of breast cancer patients. Methods for preparing and identifying immunogenic portions of antigens of known sequence are well known in the art and include those summarized in Paul,  Fundamental Immunology , 3 rd  ed., Raven Press, 1993, pp. 243-247.  
     [0189] The term “polynucleotide(s),” as used herein, means a single or double-stranded polymer of deoxyribonucleotide or ribonucleotide bases and includes DNA and corresponding RNA molecules, including HnRNA and mRNA molecules, both sense and anti-sense strands, and comprehends cDNA, genomic DNA and recombinant DNA, as well as wholly or partially synthesized polynucleotides. An HnRNA molecule contains introns and corresponds to a polynucleotide in a generally one-to-one manner. An mRNA molecule corresponds to an HnRNA and polynucleotide from which the introns have been excised. A polynucleotide may consist of an entire gene, or any portion thereof. Operable anti-sense polynucleotides may comprise a fragment of the corresponding polynucleotide, and the definition of “polynucleotide” therefore includes all such operable anti-sense fragments.  
     [0190] The compositions and methods of the present invention also encompass variants of the above polypeptides and polynucleotides. Such variants include, but are not limited to, naturally occurring allelic variants of the inventive sequences.  
     [0191] A polypeptide “variant,” as used herein, is a polypeptide that differs from the recited polypeptide only in conservative substitutions and/or modifications, such that the antigenic properties of the polypeptide are retained. In a preferred embodiment, variant polypeptides differ from an identified sequence by substitution, deletion or addition of five amino acids or fewer. Such variants may generally be identified by modifying one of the above polypeptide sequences, and evaluating the antigenic properties of the modified polypeptide using, for example, the representative procedures described herein. Polypeptide variants preferably exhibit at least about 70%, more preferably at least about 90% and most preferably at least about 95% identity (determined as described below) to the identified polypeptides.  
     [0192] As used herein, a “conservative substitution” is one in which an amino acid is substituted for another amino acid that has similar properties, such that one skilled in the art of peptide chemistry would expect the secondary structure and hydropathic nature of the polypeptide to be substantially unchanged. In general, the following groups of amino acids represent conservative changes: (1) ala, pro, gly, glu, asp, gln, asn, ser, thr; (2) cys, ser, tyr, thr; (3) val, ile, leu, met, ala, phe; (4) lys, arg, his; and (5) phe, tyr, trp, his.  
     [0193] Variants may also, or alternatively, contain other modifications, including the deletion or addition of amino acids that have minimal influence on the antigenic properties, secondary structure and hydropathic nature of the polypeptide. For example, a polypeptide may be conjugated to a signal (or leader) sequence at the N-terminal end of the protein which co-translationally or post-translationally directs transfer of the protein. The polypeptide may also be conjugated to a linker or other sequence for ease of synthesis, purification or identification of the polypeptide (e.g., poly-His), or to enhance binding of the polypeptide to a solid support. For example, a polypeptide may be conjugated to an immunoglobulin Fc region.  
     [0194] A nucleotide “variant” is a sequence that differs from the recited nucleotide sequence in having one or more nucleotide deletions, substitutions or additions. Such modifications may be readily introduced using standard mutagenesis techniques, such as oligonucleotide-directed site-specific mutagenesis as taught, for example, by Adelman et al. ( DNA,  2:183, 1983). Nucleotide variants may be naturally occurring allelic variants, or non-naturally occurring variants. Variant nucleotide sequences preferably exhibit at least about 70%, more preferably at least about 80% and most preferably at least about 90% identity (determined as described below) to the recited sequence.  
     [0195] The breast tumor antigens provided by the present invention include variants that are encoded by DNA sequences which are substantially homologous to one or more of the DNA sequences specifically recited herein. “Substantial homology,” as used herein, refers to DNA sequences that are capable of hybridizing under moderately stringent conditions. Suitable moderately stringent conditions include prewashing in a solution of 5×SSC, 0.5% SDS, 1.0 mM EDTA (pH 8.0); hybridizing at 50° C.-65° C., 5×SSC, overnight or, in the event of cross-species homology, at 45° C. with 0.5×SSC; followed by washing twice at 65° C. for 20 minutes with each of 2×, 0.5× and 0.2×SSC containing 0.1% SDS. Such hybridizing DNA sequences are also within the scope of this invention, as are nucleotide sequences that, due to code degeneracy, encode an immunogenic polypeptide that is encoded by a hybridizing DNA sequence.  
     [0196] Two nucleotide or polypeptide sequences are said to be “identical” if the sequence of nucleotides or amino acid residues in the two sequences is the same when aligned for maximum correspondence as described below. Comparisons between two sequences are typically performed by comparing the sequences over a comparison window to identify and compare local regions of sequence similarity. A “comparison window” as used herein, refers to a segment of at least about 20 contiguous positions, usually 30 to about 75, 40 to about 50, in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned.  
     [0197] Optimal alignment of sequences for comparison may be conducted using the Megalign program in the Lasergene suite of bioinformatics software (DNASTAR, Inc., Madison, Wis.), using default parameters. This program embodies several alignment schemes described in the following references: Dayhoff, M. O. (1978) A model of evolutionary change in proteins—Matrices for detecting distant relationships. In Dayhoff, M. O. (ed.) Atlas of Protein Sequence and Structure, National Biomedical Resarch Foundaiton, Washington D.C. Vol. 5, Suppl. 3, pp. 345-358; Hein J. (1990) Unified Approach to Alignment and Phylogenes pp. 626-645  Methods in Enzymology  vol. 183, Academic Press, Inc., San Diego, Calif.; Higgins, D. G. and Sharp, P. M. (1989) Fast and sensitive multiple sequence alignments on a microcomputer  CABIOS  5:151-153; Myers, E. W. and Muller W. (1988) Optimal alignments in linear space  CABIOS  4:11-17; Robinson, E. D. (1971)  Comb. Theor  11:105; Santou, N. Nes, M. (1987) The neighbor joining method. A new method for reconstructing phylogenetic trees  Mol. Biol. Evol.  4:406-425; Sneath, P. H. A. and Sokal, R. R. (1973)  Numerical Taxonomy—the Principles and Practice of Numerical Taxonomy , Freeman Press, San Francisco, Calif.; Wilbur, W. J. and Lipman, D. J. (1983) Rapid similarity searches of nucleic acid and protein data banks  Proc. Natl. Acad., Sci. USA  80:726-730.  
     [0198] Preferably, the “percentage of sequence identity” is determined by comparing two optimally aligned sequences over a window of comparison of at least 20 positions, wherein the portion of the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e. gaps) of 20 percent or less, usually 5 to 15 percent, or 10 to 12 percent, as compared to the reference sequences (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid bases or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the reference sequence (i.e. the window size) and multiplying the results by 100 to yield the percentage of sequence identity.  
     [0199] The breast tumor antigens of the present invention, and polynucleotides encoding such antigens, may be isolated from breast tumor tissue using any of a variety of methods well known in the art. DNA sequences corresponding to a gene (or a portion thereof) encoding one of the inventive breast tumor antigens may be isolated from a breast tumor cDNA library using a subtraction technique as described in detail below. Examples of such DNA sequences are provided in SEQ ID NO: 1-61, 63-175, 178 and 180. Partial DNA sequences thus obtained may be used to design oligonucleotide primers for the amplification of full-length DNA sequences in a polymerase chain reaction (PCR), using techniques well known in the art (see, for example, Mullis et al.,  Cold Spring Harbor Symp. Quant. Biol.,  51:263, 1987; Erlich ed.,  PCR Technology , Stockton Press, NY, 1989). Once a DNA sequence encoding a polypeptide is obtained, any of the above modifications may be readily introduced using standard mutagenesis techniques, such as oligonucleotide-directed site-specific mutagenesis as taught, for example, by Adelman et al. ( DNA,  2:183, 1983).  
     [0200] The breast tumor polypeptides disclosed herein may also be generated by synthetic or recombinant means. Synthetic polypeptides having fewer than about 100 amino acids, and generally fewer than about 50 amino acids, may be generated using techniques well known to those of ordinary skill in the art. For example, such polypeptides may be synthesized using any of the commercially available solid-phase techniques, such as the Merrifield solid-phase synthesis method, where amino acids are sequentially added to a growing amino acid chain (see, for example, Merrifield,  J. Am. Chem. Soc.  85:2149-2146, 1963). Equipment for automated synthesis of polypeptides is commercially available from suppliers such as Perkin Elmer/Applied BioSystems Division (Foster City, Calif.), and may be operated according to the manufacturer&#39;s instructions.  
     [0201] Alternatively, any of the above polypeptides may be produced recombinantly by inserting a DNA sequence that encodes the polypeptide into an expression vector and expressing the protein in an appropriate host. Any of a variety of expression vectors known to those of ordinary skill in the art may be employed to express recombinant polypeptides of this invention. Expression may be achieved in any appropriate host cell that has been transformed or transfected with an expression vector containing a polynucleotide that encodes a recombinant polypeptide. Suitable host cells include prokaryotes, yeast and higher eukaryotic cells. Preferably, the host cells employed are  E. coli , yeast or a mammalian cell line, such as CHO cells. The DNA sequences expressed in this manner may encode naturally occurring polypeptides, portions of naturally occurring polypeptides, or other variants thereof.  
     [0202] In general, regardless of the method of preparation, the polypeptides disclosed herein are prepared in an isolated, substantially pure, form (i.e., the polypeptides are homogenous as determined by amino acid composition and primary sequence analysis). Preferably, the polypeptides are at least about 90% pure, more preferably at least about 95% pure and most preferably at least about 99% pure. In certain preferred embodiments, described in more detail below, the substantially pure polypeptides are incorporated into pharmaceutical compositions or vaccines for use in one or more of the methods disclosed herein.  
     [0203] In a related aspect, the present invention provides fusion proteins comprising a first and a second inventive polypeptide or, alternatively, a polypeptide of the present invention and a known breast tumor antigen, together with variants of such fusion proteins.  
     [0204] A DNA sequence encoding a fusion protein of the present invention is constructed using known recombinant DNA techniques to assemble separate DNA sequences encoding the first and second polypeptides into an appropriate expression vector. The 3′ end of a DNA sequence encoding the first polypeptide is ligated, with or without a peptide linker, to the 5′ end of a DNA sequence encoding the second polypeptide so that the reading frames of the sequences are in phase to permit mRNA translation of the two DNA sequences into a single fusion protein that retains the biological activity of both the first and the second polypeptides.  
     [0205] A peptide linker sequence may be employed to separate the first and the second polypeptides by a distance sufficient to ensure that each polypeptide folds into its secondary and tertiary structures. Such a peptide linker sequence is incorporated into the fusion protein using standard techniques well known in the art. Suitable peptide linker sequences may be chosen based on the following factors: (1) their ability to adopt a flexible extended conformation; (2) their inability to adopt a secondary structure that could interact with functional epitopes on the first and second polypeptides; and (3) the lack of hydrophobic or charged residues that might react with the polypeptide functional epitopes. Preferred peptide linker sequences contain Gly, Asn and Ser residues. Other near neutral amino acids, such as Thr and Ala may also be used in the linker sequence. Amino acid sequences which may be usefully employed as linkers include those disclosed in Maratea et al.,  Gene  40:39-46, 1985; Murphy et al.,  Proc. Natl. Acad. Sci. USA  83:8258-8262, 1986; U.S. Pat. No. 4,935,233 and U.S. Pat. No. 4,751,180. The linker sequence may be from 1 to about 50 amino acids in length. Peptide sequences are not required when the first and second polypeptides have non-essential N-terminal amino acid regions that can be used to separate the functional domains and prevent steric interference.  
     [0206] The ligated DNA sequences are operably linked to suitable transcriptional or translational regulatory elements. The regulatory elements responsible for expression of DNA are located only 5′ to the DNA sequence encoding the first polypeptides. Similarly, stop codons require to end translation and transcription termination signals are only present 3′ to the DNA sequence encoding the second polypeptide.  
     [0207] Fusion proteins are also provided that comprise a polypeptide of the present invention together with an unrelated immunogenic protein. Preferably the immunogenic protein is capable of eliciting a recall response. Examples of such proteins include tetanus, tuberculosis and hepatitis proteins (see, for example, Stoute et al.  New Engl. J. Med.,  336:86-91 (1997)).  
     [0208] Polypeptides of the present invention that comprise an immunogenic portion of a breast tumor antigen may generally be used for immunotherapy of breast cancer, wherein the polypeptide stimulates the patient&#39;s own immune response to breast tumor cells. The present invention thus provides methods for using one or more of the immunoreactive polypeptides encoded by a polynucleotide comprising a sequence of SEQ ID NO: 1-61, 63-175, 178 and 180 (or fusion proteins comprising one or more such polypeptides and/or DNA encoding such polypeptides) for immunotherapy of breast cancer in a patient. As used herein, a “patient” refers to any warm-blooded animal, preferably a human. A patient may be afflicted with a disease, or may be free of detectable disease. Accordingly, the above immunoreactive polypeptides (or fusion proteins or polynucleotides encoding such polypeptides) may be used to treat breast cancer or to inhibit the development of breast cancer. The polypeptides may be administered either prior to or following surgical removal of primary tumors and/or treatment by administration of radiotherapy and conventional chemotherapeutic drugs.  
     [0209] In these aspects, the polypeptide or fusion protein is generally present within a pharmaceutical composition and/or a vaccine. Pharmaceutical compositions may comprise one or more polypeptides, each of which may contain one or more of the inventive sequences (or variants thereof), and a physiologically acceptable carrier. The vaccines may comprise one or more such polypeptides and a non-specific immune response enhancer, wherein the non-specific immune response enhancer is capable of eliciting or enhancing an immune response to an exogenous antigen. Examples of non-specific-immune response enhancers include adjuvants, biodegradable microspheres (e.g., polylactic galactide) and liposomes (into which the polypeptide is incorporated). Pharmaceutical compositions and vaccines may also contain other epitopes of breast tumor antigens, either incorporated into a combination polypeptide (i.e., a single polypeptide that contains multiple epitopes) or present within a separate polypeptide.  
     [0210] Alternatively, a pharmaceutical composition or vaccine may contain DNA encoding one or more of the above polypeptides, such that the polypeptide is generated in situ. In such pharmaceutical compositions and vaccines, the DNA may be present within any of a variety of delivery systems known to those of ordinary skill in the art, including nucleic acid expression systems, bacteria and viral expression systems. Appropriate nucleic acid expression systems contain the necessary DNA sequences for expression in the patient (such as a suitable promoter). Bacterial delivery systems involve the administration of a bacterium (such as Bacillus-Calmette-Gruerrin) that expresses an epitope of a breast tumor cell antigen on its cell surface. In a preferred embodiment, the DNA may be introduced using a viral expression system (e.g., vaccinia or other pox virus, retrovirus, or adenovirus), which may involve the use of a non-pathogenic (defective), replication competent virus. Suitable systems are disclosed, for example, in Fisher-Hoch et al.,  PNAS  86:317-321, 1989; Flexner et al.,  Ann. N.Y Acad. Sci.  569:86-103, 1989; Flexner et al.,  Vaccine  8:17-21, 1990; U.S. Pat. Nos. 4,603,112, 4,769,330, and 5,017,487; WO 89/01973; U.S. Pat. No. 4,777,127; GB 2,200,651; EP 0,345,242; WO 91/02805; Berkner,  Biotechniques  6:616-627, 1988; Rosenfeld et al.,  Science  252:431-434, 1991; Kolls et al.,  PNAS  91:215-219, 1994; Kass-Eisler et al.,  PNAS  90:11498-11502, 1993; Guzman et al.,  Circulation  88:2838-2848, 1993; and Guzman et al.,  Cir. Res.  73:1202-1207, 1993. Techniques for incorporating DNA into such expression systems are well known to those of ordinary skill in the art. The DNA may also be “naked,” as described, for example, in published PCT application WO 90/11092, and Ulmer et al.,  Science  259:1745-1749, 1993, reviewed by Cohen,  Science  259:1691-1692, 1993. The uptake of naked DNA may be increased by coating the DNA onto biodegradable beads, which are efficiently transported into the cells.  
     [0211] Routes and frequency of administration, as well as dosage, will vary from individual to individual and may parallel those currently being used in immunotherapy of other diseases. In general, the pharmaceutical compositions and vaccines may be administered by injection (e.g., intracutaneous, intramuscular, intravenous or subcutaneous), intranasally (e.g., by aspiration) or orally. Between 1 and 10 doses may be administered over a 3-24 week period. Preferably, 4 doses are administered, at an interval of 3 months, and booster administrations may be given periodically thereafter. Alternate protocols may be appropriate for individual patients. A suitable dose is an amount of polypeptide or DNA that is effective to raise an immune response (cellular and/or humoral) against breast tumor cells in a treated patient. A suitable immune response is at least 10-50% above the basal (ie., untreated) level. In general, the amount of polypeptide present in a dose (or produced in situ by the DNA in a dose) ranges from about 1 pg to about 100 mg per kg of host, typically from about 10 pg to about 1 mg, and preferably from about 100 pg to about 1 μg. Suitable dose sizes will vary with the size of the patient, but will typically range from about 0.01 mL to about 5 mL.  
     [0212] While any suitable carrier known to those of ordinary skill in the art may be employed in the pharmaceutical compositions of this invention, the type of carrier will vary depending on the mode of administration. For parenteral administration, such as subcutaneous injection, the carrier preferably comprises water, saline, alcohol, a lipid, a wax and/or a buffer. For oral administration, any of the above carriers or a solid carrier, such as mannitol, lactose, starch, magnesium stearate, sodium saccharine, talcum, cellulose, glucose, sucrose, and/or magnesium carbonate, may be employed. Biodegradable microspheres (e.g., polylactic glycolide) may also be employed as carriers for the pharmaceutical compositions of this invention. Suitable biodegradable microspheres are disclosed, for example, in U.S. Pat. Nos. 4,897,268 and 5,075,109.  
     [0213] Any of a variety of non-specific immune response enhancers may be employed in the vaccines of this invention. For example, an adjuvant may be included. Most adjuvants contain a substance designed to protect the antigen from rapid catabolism, such as aluminum hydroxide or mineral oil, and a nonspecific stimulator of immune response, such as lipid A,  Bordella pertussis  or  Mycobacterium tuberculosis . Such adjuvants are commercially available as, for example, Freund&#39;s Incomplete Adjuvant and Complete Adjuvant (Difco Laboratories, Detroit, Mich.) and Merck Adjuvant 65 (Merck and Company, Inc., Rahway, N.J.).  
     [0214] Polypeptides disclosed herein may also be employed in adoptive immunotherapy for the treatment of cancer. Adoptive immunotherapy may be broadly classified into either active or passive immunotherapy. In active immunotherapy, treatment relies on the in vivo stimulation of the endogenous host immune system to react against tumors with the administration of immune response-modifying agents (for example, tumor vaccines, bacterial adjuvants, and/or cytokines).  
     [0215] In passive immunotherapy, treatment involves the delivery of biologic reagents with established tumor-immune reactivity (such as effector cells or antibodies) that can directly or indirectly mediate antitumor effects and does not necessarily depend on an intact host immune system. Examples of effector cells include T lymphocytes (for example, CD8+ cytotoxic T-lymphocyte, CD4+ T-helper, gamma/delta T lymphocytes, tumor-infiltrating lymphocytes), killer cells (such as Natural Killer cells, lymphokine-activated killer cells), B cells, or antigen presenting cells (such as dendritic cells and macrophages) expressing the disclosed antigens. The polypeptides disclosed herein may also be used to generate antibodies or anti-idiotypic antibodies (as in U.S. Pat. No. 4,918,164), for passive immunotherapy.  
     [0216] The predominant method of procuring adequate numbers of T-cells for adoptive immunotherapy is to grow immune T-cells in vitro. Culture conditions for expanding single antigen-specific T-cells to several billion in number with retention of antigen recognition in vivo are well known in the art. These in vitro culture conditions typically utilize intermittent stimulation with antigen, often in the presence of cytokines, such as IL-2, and non-dividing feeder cells. As noted above, the immunoreactive polypeptides described herein may be used to rapidly expand antigen-specific T cell cultures in order to generate sufficient number of cells for immunotherapy. In particular, antigen-presenting cells, such as dendritic, macrophage, monocyte, fibroblast or B-cells, may be pulsed with immunoreactive polypeptides or polynucleotide sequence(s) may be introduced into: antigen presenting cells, using standard techniques well known in the art. For example, antigen presenting cells may be transfected or transduced with a polynucleotide sequence, wherein said sequence contains a promoter region appropriate for inducing expression, and can be expressed as part of a recombinant virus or other expression system. Several viral vectors may be used to transduce an antigen presenting cell, including pox virus, vaccinia virus, and adenovirus. Antigen presenting cells may be transfected with polynucleotide sequences disclosed herein by a variety of means, including gene-gun technology, lipid-mediated delivery, electroporation, osmotic shock, and particulate delivery mechanisms, resulting in efficient and acceptable expression levels as determined by one of ordinary skill in the art. For cultured T-cells to be effective in therapy, the cultured T-cells must be able to grow and distribute widely and to survive long term in vivo. Studies have demonstrated that cultured T-cells can be induced to grow in vivo and to survive long term in substantial numbers by repeated stimulation with antigen supplemented with IL-2 (see, for example, Cheever et al.  Ibid ).  
     [0217] The polypeptides disclosed herein may also be employed to generate and/or isolate tumor-reactive T-cells, which can then be administered to the patient. In one technique, antigen-specific T-cell lines may be generated by in vivo immunization with short peptides corresponding to immunogenic portions of the disclosed polypeptides. The resulting antigen specific CD8+ CTL clones may be isolated from the patient, expanded using standard tissue culture techniques, and returned to the patient.  
     [0218] Alternatively, peptides corresponding to immunogenic portions of the polypeptides may be employed to generate tumor reactive T cell subsets by selective in vitro stimulation and expansion of autologous T cells to provide antigen-specific T cells which may be subsequently transferred to the patient as described, for example, by Chang et al. ( Crit. Rev. Oncol. Hematol.,  22(3), 213, 1996). Cells of the immune system, such as T cells, may be isolated from the peripheral blood of a patient, using a commercially available cell separation system. The separated cells are stimulated with one or more of the immunoreactive polypeptides contained within a delivery vehicle, such as a microsphere, to provide antigen-specific T cells. The population of tumor antigen-specific T cells is then expanded using standard techniques and the cells are administered back to the patient.  
     [0219] In other embodiments, T-cell and/or antibody receptors specific for the polypeptides disclosed herein can be cloned, expanded, and transferred into other vectors or effector cells for use in adoptive immunotherapy. In particular, T cells may be transfected with the appropriate genes to express the variable domains from tumor specific monoclonal antibodies as the extracellular recognition elements and joined to the T cell receptor signaling chains, resulting in T cell activation, specific lysis, and cytokine release. This enables the T cell to redirect its specificity in an MHC-independent manner. See for example, Eshhar, Z.,  Cancer Immunol Immunother,  45(3-4):131-6, 1997 and Hwu, P., et al,  Cancer Res,  55(15):3369-73, 1995. Another embodiment may include the transfection of tumor antigen specific alpha and beta T cell receptor chains into alternate T cells, as in Cole, D J, et al,  Cancer Res,  55(4):748-52, 1995.  
     [0220] In further embodiments, syngeneic or autologous dendritic cells may be pulsed with peptides corresponding to at least an immunogenic portion of a polypeptide disclosed herein. The resulting antigen-specific dendritic cells may either be transferred into a patient, or employed to stimulate T cells to provide antigen-specific T cells which may, in turn, be administered to a patient. The use of peptide-pulsed dendritic cells to generate antigen-specific T cells and the subsequent use of such antigen-specific T cells to eradicate tumors in a murine model has been demonstrated by Cheever et al. (“Therapy With Cultured T Cells: Principles Revisited,”  Immunological Reviews,  157:177, 1997). Additionally vectors expressing the disclosed polynucleotides may be introduced into stem cells taken from the patient and clonally propagated in vitro for autologous transplant back into the same patient.  
     [0221] In one specific embodiment, cells of the immune system, such as T cells, may be isolated from the peripheral blood of a patient, using a commercially available cell separation system, such as CellPro Incorporated&#39;s (Bothell, Wash.) CEPRATE™ system (see U.S. Pat. No. 5,240,856; U.S. Pat. No. 5,215,926; WO 89/06280; WO 91/16116 and WO 92/07243). The separated cells are stimulated with one or more of the immunoreactive polypeptides contained within a delivery vehicle, such as a microsphere, to provide antigen-specific T cells. The population of tumor antigen-specific T cells is then expanded using standard techniques and the cells are administered back to the patient.  
     [0222] Additionally vectors expressing the disclosed polynucleotides may be introduced into stem cells taken from the patient and clonally propagated in vitro for autologous transplant back into the same patient.  
     [0223] Polypeptides of the present invention may also, or alternatively, be used to generate binding agents, such as antibodies or fragments thereof, that are capable of detecting metastatic human breast tumors. Binding agents of the present invention may generally be prepared using methods known to those of ordinary skill in the art, including the representative procedures described herein. Binding agents are capable of differentiating between patients with and without breast cancer, using the representative assays described herein. In other words, antibodies or other binding agents raised against a breast tumor antigen, or a suitable portion thereof, will generate a signal indicating the presence of primary or metastatic breast cancer in at least about 20% of patients afflicted with the disease, and will generate a negative signal indicating the absence of the disease in at least about 90% of individuals without primary or metastatic breast cancer. Suitable portions of such breast tumor antigens are portions that are able to generate a binding agent that indicates the presence of primary or metastatic breast cancer in substantially all (i.e., at least about 80%, and preferably at least about 90%) of the patients for which breast cancer would be indicated using the full length antigen, and that indicate the absence of breast cancer in substantially all of those samples that would be negative when tested with full length antigen. The representative assays described below, such as the two-antibody sandwich assay, may generally be employed for evaluating the ability of a binding agent to detect metastatic human breast tumors.  
     [0224] The ability of a polypeptide prepared as described herein to generate antibodies capable of detecting primary or metastatic human breast tumors may generally be evaluated by raising one or more antibodies against the polypeptide (using, for example, a representative method described herein) and determining the ability of such antibodies to detect such tumors in patients. This determination may be made by assaying biological samples from patients with and without primary or metastatic breast cancer for the presence of a polypeptide that binds to the generated antibodies. Such test assays may be performed, for example, using a representative procedure described below. Polypeptides that generate antibodies capable of detecting at least 20% of primary or metastatic breast tumors by such procedures are considered to be useful in assays for detecting primary or metastatic human breast tumors Polypeptide specific antibodies may be used alone or in combination to improve sensitivity.  
     [0225] Polypeptides capable of detecting primary or metastatic human breast tumors may be used as markers for diagnosing breast cancer or for monitoring disease progression in patients. In one embodiment, breast cancer in a patient may be diagnosed by evaluating a biological sample obtained from the patient for the level of one or more of the above polypeptides, relative to a predetermined cut-off value. As used herein, suitable “biological samples” include blood, sera and urine.  
     [0226] The level of one or more of the above polypeptides may be evaluated using any binding agent specific for the polypeptide(s). A “binding agent,” in the context of this invention, is any agent (such as a compound or a cell) that binds to a polypeptide as described above. As used herein, “binding” refers to a noncovalent association between two separate molecules (each of which may be free (ie., in solution) or present on the surface of a cell or a solid support), such that a “complex” is formed. Such a complex may be free or immobilized (either covalently or noncovalently) on a support material. The ability to bind may generally be evaluated by determining a binding constant for the formation of the complex. The binding constant is the value obtained when the concentration of the complex is divided by the product of the component concentrations. In general, two compounds are said to “bind” in the context of the present invention when the binding constant for complex formation exceeds about 10 3  L/mol. The binding constant may be determined using methods well known to those of ordinary skill in the art.  
     [0227] Any agent that satisfies the above requirements may be a binding agent. For example, a binding agent may be a ribosome with or without a peptide component, an RNA molecule or a peptide. In a preferred embodiment, the binding partner is an antibody, or a fragment thereof. Such antibodies may be polyclonal, or monoclonal. In addition, the antibodies may be single chain, chimeric, CDR-grafted or humanized. Antibodies may be prepared by the methods described herein and by other methods well known to those of skill in the art.  
     [0228] There are a variety of assay formats known to those of ordinary skill in the art for using a binding partner to detect polypeptide markers in a sample. See, e.g., Harlow and Lane,  Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory, 1988. In a preferred embodiment, the assay involves the use of binding partner immobilized on a solid support to bind to and remove the polypeptide from the remainder of the sample. The bound polypeptide may then be detected using a second binding partner that contains a reporter group. Suitable second binding partners include antibodies that bind to the binding partner/polypeptide complex. Alternatively, a competitive assay may be utilized, in which a polypeptide is labeled with a reporter group and allowed to bind to the immobilized binding partner after incubation of the binding partner with the sample. The extent to which components of the sample inhibit the binding of the labeled polypeptide to the binding partner is indicative of the reactivity of the sample with the immobilized binding partner.  
     [0229] The solid support may be any material known to those of ordinary skill in the art to which the antigen may be attached. For example, the solid support may be a test well in a microtiter plate or a nitrocellulose or other suitable membrane. Alternatively, the support may be a bead or disc, such as glass, fiberglass, latex or a plastic material such as polystyrene or polyvinylchloride. The support may also be a magnetic particle or a fiber optic sensor, such as those disclosed, for example, in U.S. Pat. No. 5,359,681. The binding agent may be immobilized on the solid support using a variety of techniques known to those of skill in the art, which are amply described in the patent and scientific literature. In the context of the present invention, the term “immobilization” refers to both noncovalent association, such as adsorption, and covalent attachment (which may be a direct linkage between the antigen and functional groups on the support or may be a linkage by way of a cross-linking agent). Immobilization by adsorption to a well in a microtiter plate or to a membrane is preferred. In such cases, adsorption may be achieved by contacting the binding agent, in a suitable buffer, with the solid support for a suitable amount of time. The contact time varies with temperature, but is typically between about 1 hour and about 1 day. In general, contacting a well of a plastic microtiter plate (such as polystyrene or polyvinylchloride) with an amount of binding agent ranging from about 10 ng to about 10 μg, and preferably about 100 ng to about 1 μg,; is sufficient to immobilize an adequate amount of binding agent.  
     [0230] Covalent attachment of binding agent to a solid support may generally be achieved by first reacting the support with a bifunctional reagent that will react with both the support and a functional group, such as a hydroxyl or amino group, on the binding agent. For example, the binding agent may be covalently attached to supports having an appropriate polymer coating using benzoquinone or by condensation of an aldehyde group on the support with an amine and an active hydrogen on the binding partner (see, e.g., Pierce Immunotechnology Catalog and Handbook, 1991, at A12-A13).  
     [0231] In certain embodiments, the assay is a two-antibody sandwich assay. This assay may be performed by first contacting an antibody that has been immobilized on a solid support, commonly the well of a microtiter plate, with the sample, such that polypeptides within the sample are allowed to bind to the immobilized antibody. Unbound sample is then removed from the immobilized polypeptide-antibody complexes and a second antibody (containing a reporter group) capable of binding to a different site on the polypeptide is added. The amount of second antibody that remains bound to the solid support is then determined using a method appropriate for the specific reporter group.  
     [0232] More specifically, once the antibody is immobilized on the support as described above, the remaining protein binding sites on the support are typically blocked. Any suitable blocking agent known to those of ordinary skill in the art, such as bovine serum albumin or Tween 20™ (Sigma Chemical Co., St. Louis, Mo.). The immobilized antibody is then incubated with the sample, and polypeptide is allowed to bind to the antibody. The sample may be diluted with a suitable diluent, such as phosphate-buffered saline (PBS) prior to incubation. In general, an appropriate contact time (i.e., incubation time) is that period of time that is sufficient to detect the presence of polypeptide within a sample obtained from an individual with breast cancer. Preferably, the contact time is sufficient to achieve a level of binding that is at least about 95% of that achieved at equilibrium between bound and unbound polypeptide. Those of ordinary skill in the art will recognize that the time necessary to achieve equilibrium may be readily determined by assaying the level of binding that occurs over a period of time. At room temperature, an incubation time of about 30 minutes is generally sufficient.  
     [0233] Unbound sample may then be removed by washing the solid support with an appropriate buffer, such as PBS containing 0.1% Tween 20™. The second antibody, which contains a reporter group, may then be added to the solid support. Preferred reporter groups include enzymes (such as horseradish peroxidase), substrates, cofactors, inhibitors, dyes, radionuclides, luminescent groups, fluorescent groups and biotin. The conjugation of antibody to reporter group may be achieved using standard methods known to those of ordinary skill in the art.  
     [0234] The second antibody is then incubated with the immobilized antibody-polypeptide complex for an amount of time sufficient to detect the bound polypeptide. An appropriate amount of time may generally be determined by assaying the level of binding that occurs over a period of time. Unbound second antibody is then removed and bound second antibody is detected using the reporter group. The method employed for detecting the reporter group depends upon the nature of the reporter group. For radioactive groups, scintillation counting or autoradiographic methods are generally appropriate. Spectroscopic methods may be used to detect dyes, luminescent groups and fluorescent groups. Biotin may be detected using avidin, coupled to a different reporter group (commonly a radioactive or fluorescent group or an enzyme). Enzyme reporter groups may generally be detected by the addition of substrate (generally for a specific period of time), followed by spectroscopic or other analysis of the reaction products.  
     [0235] To determine the presence or absence of breast cancer, the signal detected from the reporter group that remains bound to the solid support is generally compared to a signal that corresponds to a predetermined cut-off value. In one preferred embodiment, the cut-off value is the average mean signal obtained when the immobilized antibody is incubated with samples from patients without breast cancer. In general, a sample generating a signal that is three standard deviations above the predetermined cut-off value is considered positive for breast cancer. In an alternate preferred embodiment, the cut-off value is determined using a Receiver Operator Curve, according to the method of Sackett et al.,  Clinical Epidemiology: A Basic Science for Clinical Medicine , Little Brown and Co., 1985, p. 106-7. Briefly, in this embodiment, the cut-off value may be determined from a plot of pairs of true positive rates (i.e., sensitivity) and false positive rates (100%-specificity) that correspond to each possible cut-off value for the diagnostic test result. The cut-off value on the plot that is the closest to the upper left-hand corner (i.e., the value that encloses the largest area) is the most accurate cut-off value, and a sample generating a signal that is higher than the cut-off value determined by this method may be considered positive. Alternatively, the cut-off value may be shifted to the left along the plot, to minimize the false positive rate, or to the right, to minimize the false negative rate. In general, a sample generating a signal that is higher than the cut-off value determined by this method is considered positive for breast cancer.  
     [0236] In a related embodiment, the assay is performed in a flow-through or strip test format, wherein the antibody is immobilized on a membrane, such as nitrocellulose. In the flow-through test, polypeptides within the sample bind to the immobilized antibody as the sample passes through the membrane. A second, labeled antibody then binds to the antibody-polypeptide complex as a solution containing the second antibody flows through the membrane. The detection of bound second antibody may then be performed as described above. In the strip test format, one end of the membrane to which antibody is bound is immersed in a solution containing the sample. The sample migrates along the membrane through a region containing second antibody and to the area of immobilized antibody. Concentration of second antibody at the area of immobilized antibody indicates the presence of breast cancer. Typically, the concentration of second antibody at that site generates a pattern, such as a line, that can be read visually. The absence of such a pattern indicates a negative result. In general, the amount of antibody immobilized on the membrane is selected to generate a visually discernible pattern when the biological sample contains a level of polypeptide that would be sufficient to generate a positive signal in the two-antibody sandwich assay, in the format discussed above. Preferably, the amount of antibody immobilized on the membrane ranges from about 25 ng to about 1 μg, and more preferably from about 50 ng to about 500 ng. Such tests can typically be performed with a very small amount of biological sample.  
     [0237] Of course, numerous other assay protocols exist that are suitable for use with the antigens or antibodies of the present invention. The above descriptions are intended to be exemplary only.  
     [0238] In another embodiment, the above polypeptides may be used as markers for the progression of breast cancer. In this embodiment, assays as described above for the diagnosis of breast cancer may be performed over time, and the change in the level of reactive polypeptide(s) evaluated. For example, the assays may be performed every 24-72 hours for a period of 6 months to 1 year, and thereafter performed as needed. In general, breast cancer is progressing in those patients in whom the level of polypeptide detected by the binding agent increases over time. In contrast, breast cancer is not progressing when the level of reactive polypeptide either remains constant or decreases with time.  
     [0239] Antibodies for use in the above methods may be prepared by any of a variety of techniques known to those of ordinary skill in the art. See, e.g., Harlow and Lane,  Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory, 1988. In one such technique, an immunogen comprising the antigenic polypeptide is initially injected into any of a wide variety of mammals (e.g., mice, rats, rabbits; sheep and goats). In this step, the polypeptides of this invention may serve as the immunogen without modification. Alternatively, particularly for relatively short polypeptides, a superior immune response may be elicited if the polypeptide is joined to a carrier protein, such as bovine serum albumin or keyhole limpet hemocyanin. The immunogen is injected into the animal host, preferably according to a predetermined schedule incorporating one or more booster immunizations, and the animals are bled periodically. Polyclonal antibodies specific for the polypeptide may then be purified from such antisera by, for example, affinity chromatography using the polypeptide coupled to a suitable solid support.  
     [0240] Monoclonal antibodies specific for the antigenic polypeptide of interest may be prepared, for example, using the technique of Kohler and Milstein,  Eur. J. Immunol.  6:511-519, 1976, and improvements thereto. Briefly, these methods involve the preparation of immortal cell lines capable of producing antibodies having the desired specificity (i.e., reactivity with the polypeptide of interest). Such cell lines may be produced, for example, from spleen cells obtained from an animal immunized as described above. The spleen cells are then immortalized by, for example, fusion with a myeloma cell fusion partner, preferably one that is syngeneic with the immunized animal. A variety of fusion techniques may be employed. For example, the spleen cells and myeloma cells may be combined with a nonionic detergent for a few minutes and then plated at low density on a selective medium that supports the growth of hybrid cells, but not myeloma cells. A preferred selection technique uses HAT (hypoxanthine, aminopterin, thymidine) selection. After a sufficient time, usually about 1 to 2 weeks, colonies of hybrids are observed. Single colonies are selected and tested for binding activity against the polypeptide. Hybridomas having high reactivity and specificity are preferred.  
     [0241] Monoclonal antibodies may be isolated from the supernatants of growing hybridoma colonies. In addition, various techniques may be employed to enhance the yield, such as injection of the hybridoma cell line into the peritoneal cavity of a suitable vertebrate host, such as a mouse. Monoclonal antibodies may then be harvested from the ascites fluid or the blood. Contaminants may be removed from the antibodies by conventional techniques, such as chromatography, gel filtration, precipitation, and extraction. The polypeptides of this invention may be used in the purification process in, for example, an affinity chromatography step.  
     [0242] Monoclonal antibodies of the present invention may also be used as therapeutic reagents, to diminish or eliminate breast tumors. The antibodies may be used on their own (for instance, to inhibit metastases) or coupled to one or more therapeutic agents. Suitable agents in this regard include radionuclides, differentiation inducers, drugs, toxins, and derivatives thereof. Preferred radionuclides include  90 Y,  123 I,  125 I,  131 I,  186 Re,  188 Re,  211 At, and  212 Bi. Preferred drugs include methotrexate, and pyrimidine and purine analogs. Preferred differentiation inducers include phorbol esters and butyric acid. Preferred toxins include ricin, abrin, diptheria toxin, cholera toxin, gelonin,  Pseudomonas exotoxin, Shigella toxin , and pokeweed antiviral protein.  
     [0243] A therapeutic agent may be coupled (e.g., covalently bonded) to a suitable monoclonal antibody either directly or indirectly (e.g., via a linker group). A direct reaction between an agent and an antibody is possible when each possesses a substituent capable of reacting with the other. For example, a nucleophilic group, such as an amino or sulfhydryl group, on one may be capable of reacting with a carbonyl-containing group, such as an anhydride or an acid halide, or with an alkyl group containing a good leaving group (e.g., a halide) on the other.  
     [0244] Alternatively, it may be desirable to couple a therapeutic agent and an antibody via a linker group. A linker group can function as a spacer to distance an antibody from an agent in order to avoid interference with binding capabilities. A linker group can also serve to increase the chemical reactivity of a substituent on an agent or an antibody, and thus increase the coupling efficiency. An increase in chemical reactivity may also facilitate the use of agents, or functional groups on agents, which otherwise would not be possible.  
     [0245] It will be evident to those skilled in the art that a variety of bifunctional or polyfunctional reagents, both homo- and hetero-functional (such as those described in the catalog of the Pierce Chemical Co., Rockford, Ill.), may be employed as the linker group. Coupling may be effected, for example, through amino groups, carboxyl groups, sulfhydryl groups or oxidized carbohydrate residues. There are numerous references describing such methodology, e.g., U.S. Pat. No. 4,671,958, to Rodwell et al.  
     [0246] Where a therapeutic agent is more potent when free from the antibody portion of the immunoconjugates of the present invention, it may be desirable to use a linker group which is cleavable during or upon internalization into a cell. A number of different cleavable linker groups have been described. The mechanisms for the intracellular release of an agent from these linker groups include cleavage by reduction of a disulfide bond (e.g., U.S. Pat. No. 4,489,710, to Spitler), by irradiation of a photolabile bond (e.g., U.S. Pat. No. 4,625,014, to Senter et al.), by hydrolysis of derivatized amino acid side chains (e.g., U.S. Pat. No. 4,638,045, to Kohn et al.), by serum complement-mediated hydrolysis (e.g., U.S. Pat. No. 4,671,958, to Rodwell et al.), and acid-catalyzed hydrolysis (e.g., U.S. Pat. No. 4,569,789, to Blattler et al.).  
     [0247] It may be desirable to couple more than one agent to an antibody. In one embodiment, multiple molecules of an agent are coupled to one antibody molecule. In another embodiment, more than one type of agent may be coupled to one antibody. Regardless of the particular embodiment, immunoconjugates with more than one agent may be prepared in a variety of ways. For example, more than one agent may be coupled directly to an antibody molecule, or linkers which provide multiple sites for attachment can be used. Alternatively, a carrier can be used.  
     [0248] A carrier may bear the agents in a variety of ways, including covalent bonding either directly or via a linker group. Suitable carriers include proteins such as albumins (e.g., U.S. Pat. No. 4,507,234, to Kato et al.), peptides and polysaccharides such as aminodextran (e.g., U.S. Pat. No. 4,699,784, to Shih et al.). A carrier may also bear an agent by noncovalent bonding or by encapsulation, such as within a liposome vesicle (e.g., U.S. Pat. Nos. 4,429,008 and 4,873,088). Carriers specific for radionuclide agents include radiohalogenated small molecules and chelating compounds. For example, U.S. Pat. No. 4,735,792 discloses representative radiohalogenated small molecules and their synthesis. A radionuclide chelate may be formed from chelating compounds that include those containing nitrogen and sulfur atoms as the donor atoms for binding the metal, or metal oxide, radionuclide. For example, U.S. Pat. No. 4,673,562, to Davison et al. discloses representative chelating compounds and their synthesis.  
     [0249] A variety of routes of administration for the antibodies and immunoconjugates may be used. Typically, administration will be intravenous, intramuscular, subcutaneous or in the bed of a resected tumor. It will be evident that the precise dose of the antibody/immunoconjugate will vary depending upon the antibody used, the antigen density on the tumor, and the rate of clearance of the antibody.  
     [0250] Diagnostic reagents of the present invention may also comprise DNA sequences encoding one or more of the above polypeptides, or one or more portions thereof. For example, at least two oligonucleotide primers may be employed in a polymerase chain reaction (PCR) based assay to amplify breast tumor-specific cDNA derived from a biological sample, wherein at least one of the oligonucleotide primers is specific for a polynucleotide encoding a breast tumor protein of the present invention. The presence of the amplified cDNA is then detected using techniques well known in the art, such as gel electrophoresis. Similarly, oligonucleotide probes specific for a polynucleotide encoding a breast tumor protein of the present invention may be used in a hybridization assay to detect the presence of an inventive polypeptide in a biological sample.  
     [0251] As used herein, the term “oligonucleotide primer/probe specific for a polynucleotide” means an oligonucleotide sequence that has at least about 60%, preferably at least about 75% and more preferably at least about 90%, identity to the polynucleotide in question. Oligonucleotide primers and/or probes which may be usefully employed in the inventive diagnostic methods preferably have at least about 10-40 nucleotides. In a preferred embodiment, the oligonucleotide primers comprise at least about 10 contiguous nucleotides of a polynucleotide comprising a sequence selected from SEQ ID NO: 1-61, 63-175, 178 and 180. Preferably, oligonucleotide probes for use in the inventive diagnostic methods comprise at least about 15 contiguous oligonucleotides of a polynucleotide comprising a sequence provided in SEQ ID NO: 1-61, 63-175, 178 and 180. Techniques for both PCR based assays and hybridization assays are well known in the art (see, for example, Mullis et al.  Ibid ; Ehrlich,  Ibid ). Primers or probes may thus be used to detect breast tumor-specific sequences in biological samples, including blood, urine and/or breast tumor tissue.  
     [0252] The following Examples are offered by way of illustration and not by way of limitation.  
     EXAMPLES  
     Example 1  
     [0253] Isolation and Characterization of Breast Tumor Polypeptides  
     [0254] This Example describes the isolation of breast tumor polypeptides from a breast tumor cDNA library.  
     [0255] A cDNA subtraction library containing cDNA from breast tumor subtracted with normal breast cDNA was constructed as follows. Total RNA was extracted from primary tissues using Trizol reagent (Gibco BRL Life Technologies, Gaithersburg, Md.) as described by the manufacturer. The polyA+RNA was purified using an oligo(dT) cellulose column according to standard protocols. First strand cDNA was synthesized using the primer supplied in a Clontech PCR-Select cDNA Subtraction Kit (Clontech, Palo Alto, Calif.). The driver DNA consisted of cDNAs from two normal breast tissues with the tester cDNA being from three primary breast tumors. Double-stranded cDNA was synthesized for both tester and driver, and digested with a combination of endonucleases (MluI, MscI, PvuII, SalI and StuI) which recognize six base pairs DNA. This modification increased the average cDNA size dramatically compared with cDNAs generated according to Clontech&#39;s protocol. The digested tester cDNAs were ligated to two different adaptors and the subtraction was performed according to Clontech&#39;s protocol. The subtracted cDNAs were subjected to two rounds of PCR amplification, following the manufacturer&#39;s protocol. The resulting PCR products were subcloned into the TA cloning vector, pCRII (Invitrogen, San Diego, Calif.) and transformed into ElectroMax  E. coli  DH10B cells (Gibco BRL Life, Technologies) by electroporation. DNA was isolated from independent clones and sequenced using a Perkin Elmer/Applied Biosystems Division (Foster City, Calif.) Automated Sequencer Model 373A.  
     [0256] Sixty-three distinct cDNA clones were found in the subtracted breast tumor-specific cDNA library. The determined one strand (5′ or 3′) cDNA sequences for the clones are provided in SEQ ID NO: 1-61, 72 and 73, respectively. Comparison of these cDNA sequences with known sequences in the gene bank using the EMBL and GenBank databases (Release 97) revealed no significant homologies to the sequences provided in SEQ ID NO: 14, 21, 22, 27, 29, 30, 32, 38, 44, 45, 53, 72 and 73. The sequences of SEQ ID NO: 1, 3, 16, 17, 34, 48, 57, 60 and 61 were found to represent known human genes. The sequences of SEQ ID NO: 2, 4, 23, 39 and 50 were found to show some similarity to previously identified non-human genes. The remaining clones (SEQ ID NO: 5-13, 15, 18-20, 24-26, 28, 31, 33, 35-37, 40-43, 46, 47, 49, 51, 52, 54-56, 58 and 59) were found to show at least some degree of homology to previously identified expressed sequence tags (ESTs).  
     [0257] To determine mRNA expression levels of the isolated cDNA clones, cDNA clones from the breast subtraction described above were randomly picked and colony PCR amplified. Their mRNA expression levels in breast tumor, normal breast and various other normal tissues were determined using microarray technology (Synteni, Palo Alto, Calif.). Briefly, the PCR amplification products were arrayed onto slides in an array format, with each product occupying a unique location in the array. mRNA was extracted from the tissue sample to be tested, reverse transcribed, and fluorescent-labeled cDNA probes were generated. The microarrays were probed with the labeled cDNA probes, the slides scanned and fluorescence intensity was measured. Data was analyzed using Synteni provided GEMTOOLS Software. Of the seventeen cDNA clones examined, those of SEQ ID NO: 40, 46, 59 and 73 were found to be over-expressed in breast tumor and expressed at low levels in all normal tissues tested (breast, PBMC, colon, fetal tissue, salivary gland, bone marrow, lung, pancreas, large intestine, spinal cord, adrenal gland, kidney, pancreas, liver, stomach, skeletal muscle, heart, small intestine, skin, brain and human mammary epithelial cells). The clones of SEQ ID NO: 41 and 48 were found to be over-expressed in breast tumor and expressed at low levels in all other tissues tested, with the exception of bone marrow. The clone of SEQ ID NO: 42 was found to be over-expressed in breast tumor and expressed at low levels in all other tissues tested except bone marrow and spinal cord. The clone of SEQ ID NO: 43 was found to be over-expressed in breast tumor and expressed at low levels in all other tissues tested with the exception of spinal cord, heart and small intestine The clone of SEQ ID NO: 51 was found to be over-expressed in breast tumor and expressed at low levels in all other tissues tested with the exception of large intestine. The clone of SEQ ID NO: 54 was found to be over-expressed in breast tumor and expressed at low levels in all other tissues tested with the exception of PBMC, stomach and small intestine. The clone of SEQ ID NO: 56 was found to be over-expressed in breast tumor and expressed at low levels in all other tissues tested with the exception of large and small intestine, human mammary epithelia cells and SCID mouse-passaged breast tumor. The clone of SEQ ID NO: 60 was found to be over-expressed in breast tumor and expressed at low levels in all other tissues tested with the exception of spinal cord and heart. The clone of SEQ ID NO: 61 was found to be over-expressed in breast tumor and expressed at low levels in all other tissues tested with the exception of small intestine. The clone of SEQ ID NO: 72 was found to be over-expressed in breast tumor and expressed at low levels in all other tissues tested with the exception of colon and salivary gland.  
     [0258] The results of a Northern blot analysis of the clone SYN18C6 (SEQ ID NO: 40) are shown in FIG. 1. A predicted protein sequence encoded by SYN18C6 is provided in SEQ ID NO: 62.  
     [0259] Additional cDNA clones that are over-expressed in breast tumor tissue were isolated from breast cDNA subtraction libraries as follows. Breast subtraction libraries were prepared, as described above, by PCR-based subtraction employing pools of breast tumor cDNA as the tester and pools of either normal breast cDNA or cDNA from other normal tissues as the driver. cDNA clones from breast subtraction were randomly picked and colony PCR amplified and their mRNA expression levels in breast tumor, normal breast and various other normal tissues were determined using the microarray technology described above. Twenty-four distinct cDNA clones were found to be over-expressed in breast tumor and expressed at low levels in all normal tissues tested (breast, brain, liver, pancreas, lung, salivary gland, stomach, colon, kidney, bone marrow, skeletal muscle, PBMC, heart, small intestine, adrenal gland, spinal cord, large intestine and skin). The determined partial cDNA sequences for these clones are provided in SEQ ID NO: 63-87. Comparison of the sequences of SEQ ID NO: 74-87 with those in the gene bank as described above, revealed homology to previously identified human genes. No significant homologies were found to the sequences of SEQ ID NO: 63-73.  
     [0260] Three DNA isoforms for the clone B726P (partial sequence provided in SEQ ID NO: 71) were isolated as follows. A radioactive probe was synthesized from B726P by excising B726P DNA from a pT7Blue vector (Novagen) by a BamHI/XbaI restriction digest and using the resulting DNA as the template in a single-stranded PCR in the presence of [α-32P]dCTP. The sequence of the primer employed for this PCR is provided in SEQ ID NO: 177. The resulting radioactive probe was used to probe a directional cDNA library and a random-primed cDNA library made using RNA isolated from breast tumors. Eighty-five clones were identified, excised, purified and sequenced. Of these 85 clones, three were found to each contain a significant open reading frame. The determined cDNA sequence of the isoform B726P-20 is provided in SEQ ID NO: 175, with the corresponding predicted amino acid sequence being provided in SEQ ID NO: 176. The determined cDNA sequence of the isoform B726P-74 is provided in SEQ ID NO: 178, with the corresponding predicted amino acid sequence being provided in SEQ ID NO: 179. The determined cDNA sequence of the isoform B726P-79 is provided in SEQ ID NO: 180, with the corresponding predicted amino acid sequence being provided in SEQ ID NO: 181.  
     Example 2  
     [0261] Isolation and Characterization of Breast Tumor Polypeptides Obtained by PCR-Based Subtraction Using SCID-Passaged Tumor RNA  
     [0262] Human breast tumor antigens were obtained by PCR-based subtraction using SCID mouse passaged breast tumor RNA as follows. Human breast tumor was implanted in SCID mice and harvested on the first or sixth serial passage, as described in patent application Ser. No. 08/556,659 filed Nov. 13, 1995, U.S. Pat. No. ______. Genes found to be differentially expressed between early and late passage SCID tumor may be stage specific and therefore useful in therapeutic and diagnostic applications. Total RNA was prepared from snap frozen SCID passaged human breast tumor from both the first and sixth passage.  
     [0263] PCR-based subtraction was performed essentially as described above. In the first subtraction (referred to as T9), RNA from first passage tumor was subtracted from sixth passage tumor RNA to identify more aggressive, later passage-specific antigens. Of the 64 clones isolated and sequenced from this subtraction, no significant homologies were found to 30 of these clones, hereinafter referred to as: 13053, 13057, 13059, 13065, 13067, 13068, 13071-13073, 13075, 13078, 13079, 13081, 13082, 13092, 13097, 13101, 13102, 13131, 13133, 13119, 13135, 13139, 13140, 13146-13149, and 13151, with the exception of some previously identified expressed sequence tags (ESTs). The determined cDNA sequences for these clones are provided in SEQ ID NO: 88-116, respectively. The isolated cDNA sequences of SEQ ID NO: 117-140 showed homology to known genes.  
     [0264] In a second PCR-based subtraction, RNA from sixth passage tumor was subtracted from first passage tumor RNA to identify antigens down-regulated over multiple passages. Of the 36 clones isolated and sequenced, no significant homologies were found to nineteen of these clones, hereinafter referred to as: 14376, 14377, 14383, 14384, 14387, 14392, 14394, 14398, 14401, 14402, 14405, 14409, 14412, 14414-14416, 14419, 14426, and 14427, with the exception of some previously identified expressed sequence tags (ESTs). The determined cDNA sequences for these clones are provided in SEQ ID NO: 141-159, respectively. The isolated cDNA sequences of SEQ ID NO: 160-174 were found to show homology to previously known genes.  
     Example 3  
     [0265] Synthesis of Polypeptides  
     [0266] Polypeptides may be synthesized on an Perkin Elmer/Applied Biosystems Division 430A peptide synthesizer using FMOC chemistry with HPTU (O-Benzotriazole-N,N,N′,N′-tetramethyluronium hexafluorophosphate) activation. A Gly-Cys-Gly sequence may be attached to the amino terminus of the peptide to provide a method of conjugation, binding to an immobilized surface, or labeling of the peptide. Cleavage of the peptides from the solid support may be carried out using the following cleavage mixture: trifluoroacetic acid:ethanedithiol:thioanisole:water:phenol (40:1:2:2:3). After cleaving for 2 hours, the peptides may be precipitated in cold methyl-t-butyl-ether. The peptide pellets may then be dissolved in water containing 0.1% trifluoroacetic acid (TFA) and lyophilized prior to purification by C18 reverse phase HPLC. A gradient of 0%-60% acetonitrile (containing 0.1% TFA) in water (containing 0.1% TFA) may be used to elute the peptides. Following lyophilization of the pure fractions, the peptides may be characterized using electrospray or other types of mass spectrometry and by amino acid analysis.  
     [0267] From the foregoing, it will be appreciated that, although specific embodiments of the invention have been described herein for the purposes of illustration, various modifications may be made without deviating from the spirit and scope of the invention.  
    
     
       
         1 
         
           
             181  
           
           
             1  
             281  
             DNA  
             Homo sapien  
           
            1 

caatgacagt caatctctat cgacagcctg cttcatattt agctattgtt cgtattgcct     60 

tctgtcctag gaacagtcat atctcaagtt caaatgccac aacctgagaa gcggtgggct    120 

aagataggtc ctactgcaaa ccacccctcc atatttccgt acgcaattac aattcagttt    180 

ctgtgacatc tctttacacc actggaggaa aaatgagata ttctctgatt tattctacta    240 

taacactcta catagagcta tggtgagtgc taaccacatc g                        281 

 
           
             2  
             300  
             DNA  
             Homo sapien  
           
            2 

gaggtcctgg gctaacctaa tggtttatta ttggtggaga gaaagatctg gaaatacttg     60 

aggttattac atactagatt agcttctaat gtgaaccatt tttcttttaa cagtgataaa    120 

ttattatttc cgaagttaac tgttcccttg gtcgtgatac acactcgatt aacaaacata    180 

ctgttgtatt ttttccagtt ttgtttggct atgccaccac agtcatcccc agggtctata    240 

catactatgt ctcaactgta ttatttgcca tttttggcat tagaatgctt cgggaaggct    300 

 
           
             3  
             302  
             DNA  
             Homo sapien  
           
            3 

ggccgaggta attggttaag tctaaagaga ttattattcc ttgatgtttg ctttgtattg     60 

gctacaaatg tgcagaggta atacatatgt gatgtcgatg tctctgtctt tttttttgtc    120 

tttaaaaaat aattggcagc aactgtattt gaataaaatg atttcttagt atgattgtac    180 

agtaatgaat gaaagtggaa catgtttctt tttgaaaggg agagaattga ccatttattg    240 

ttgtgatgtt taagttataa cttatcgagc acttttagta gtgataactg tttttaaact    300 

tg                                                                   302 

 
           
             4  
             293  
             DNA  
             Homo sapien  
           
            4 

tgtaccaatc ctttggcaca agaatatgta agaactatag ttgtttttat tggtttttgt     60 

tcttgagatt gttttcattc tgtttttgac tgtatctctt taggaggctg aggatggcat    120 

tattgcttat gatgactgtg gggtgaaact gactattgct tttcaagcca aggatgtgga    180 

aggatctact tctcctcaaa tacgagataa ggcaagataa ttctgctcat tcgagagagg    240 

gttaagagtt gtcatcttaa tcataaatcc tgcaggatgg gttcttcaaa ttt           293 

 
           
             5  
             275  
             DNA  
             Homo sapien  
           
            5 

cgaggtttgg aatcagactt ctgtgtccag taaaaaactc ctgcactgaa gtcattgtga     60 

cttgagtagt tacagactga ttccagtgaa cttgatctaa tttcttttga tctaatgaat    120 

gtgtctgctt accttgtctc cttttaattg ataagctcca agtagttgct aattttttga    180 

caactttaaa tgagtttcat tcacttcttt tacttaatgt tttaagtata gtaccaataa    240 

tttcattaac ctgttctcaa gtggtttagc tacca                               275 

 
           
             6  
             301  
             DNA  
             Homo sapien  
           
            6 

gaggtctggt ttcctgggta tgcctggact gttgcccagt gtaagatctg tgcaagccat     60 

attggatgga agtttacggc caccaaaaaa gacatgtcac ctcaaaaatt ttggggctta    120 

acgcgatctg ctctgttgcc cacgatccca gacactgaag atgaaataag tccagacaaa    180 

gtaatacttt gcttgtaaac agatgtgata gagataaagt tatctaacaa attggttata    240 

ttctaagatc tgctttggaa attattgcct ctgatacata cctaagtaaa cataacatta    300 

a                                                                    301 

 
           
             7  
             301  
             DNA  
             Homo sapien  
           
            7 

gtccagtttg tacacagtga ttccttatgc acgccgaaag ggtttccgta aaaatgacat     60 

tatatacaaa tctgtacacc catccaccag agcgattctc cagctcccag agggagttat    120 

caacttaaag caggatacct gaggtttcat gtctttagtt gccttatcat aatcccaaat    180 

atacatttca gggtttgttt ttgtttttaa agacactttc ctggaatatg tgcactatgg    240 

ttaaaattaa aaacaaaagt aataaaataa aatgatcgct ggaaggactg acctccccac    300 

c                                                                    301 

 
           
             8  
             301  
             DNA  
             Homo sapien  
           
            8 

ctgtcctcat ctctgcaaag ttcagcttcc ttccccaggt ctctgtgcac tctgtcttgg     60 

atgctctggg gagctcatgg gtggaggagt ctccaccaga gggaggctca ggggactggt    120 

tgggccaggg atgaatattt gagggataaa aattgtgtaa gagccaaaga attggtagta    180 

gggggagaac agagaggagc tgggctatgg gaaatgattt gaataatgga gctgggaata    240 

tggctggata tctggtacta aaaaagggtc tttaagaacc tacttcctaa tctcttcccc    300 

a                                                                    301 

 
           
             9  
             301  
             DNA  
             Homo sapien  
           
            9 

gaggtctgcc taagtagagg acaaagactt cctcctttca aaggagaact gagcccagga     60 

ttggtaagtt taaggcactt aaccttgacc agctctgtag gtctggagca ttctggtccc    120 

tggccgcttt caccaccagg cccttctcac ttatccacct cacatactgc cccagcattc    180 

ctttggcatt gcgagctgtg acttgacaca ttttaatgac aagattgaag tagctacctt    240 

gcaggataga ttttctgggg tataggggac aaaccaacag tgccatcagg tgtcttaaca    300 

c                                                                    301 

 
           
             10  
             301  
             DNA  
             Homo sapien  
           
            10 

ggcaggtcca acagttcttc cagttctggt cgagctttga atcgtccctt gaagtcttct     60 

tcagtgtgct ccttcactga cagtctgact ccttcaggaa gactgctttg gattatttcc    120 

aagaaaattt ctgcaaacgt agcactcaaa ccgctgatct gaaccactcg ctcatgggtg    180 

gtaagcactg agtccaggag cattttgctg ccttggtcct gcaactgcaa cacttctatg    240 

gttttggttg gcattgcata actttcctcg actttaatgg agagagattg cagaggttgt    300 

g                                                                    301 

 
           
             11  
             301  
             DNA  
             Homo sapien  
           
            11 

aggtctgtga ctttcaccca ggacccagga cgcagccctc cgtgggcact gccggcgcct     60 

tgtctgcaca ctggaggtcc tccattacag aggcccagcg cacatcgctg gccccacaaa    120 

cgttcagggg tacagccatg gcagctcctt cctctgccgt gagaaaagtg cttggagtac    180 

ggtttgccac acacgtgact ggacagtgtc caattcaaat ctttcagggc agagtccgag    240 

cagcgcttgg tgacagcctg tcctctcctg ctctccaaag gccctgctcc ctgtcctctc    300 

t                                                                    301 

 
           
             12  
             301  
             DNA  
             Homo sapien  
           
            12 

gaggtctggg attacaggca cgtgccacca cacctagcta atttttgagc atggggctca     60 

aaggaactgc tctctggggc atgtcagatt tcggatttgg ggctgcacac tgatactctc    120 

taagtggtgg aggaacttca tcccactgaa attcctttgg catttggggt tttgtttttc    180 

tttttttcct tcttcatcct cctccttttt taaaagtcaa cgagagcctt cgctgactcc    240 

accgaagaag tgcaccactg ggagccaccc cagtgccagg cgcccgtcca gggacacaca    300 

c                                                                    301 

 
           
             13  
             256  
             DNA  
             Homo sapien  
           
            13 

ttttttggca taaaaaacac aatgatttaa tttctaaagc acttatatta ttatggcatg     60 

gtttgggaaa caggttatta tattccacat aggtaattat gcagtgcttc tcatggaaaa    120 

aatgcttagg tattggcctt ttctctggaa accatatttt tcctttttta ataatcaact    180 

aaaatgtata tgttaaaaag cctcatcttt tgattttcaa tatacaaaat gctttcttta    240 

aaagaacaag attcaa                                                    256 

 
           
             14  
             301  
             DNA  
             Homo sapien  
           
            14 

ggtccttgat agaggaagag gaatatccaa ggcaaagcca ccaccacgtc caacctcctc     60 

atcctctacc tttcctgtcc ccagaggtat gagatagacc ccctggcctg gttcctgcac    120 

tgtgctaggc ccacagtgga cacttccacc ttaatggaga ataggcccca tggagtggag    180 

gtccctcctc catggcctgc aacccaatga ctatgggggt gacacaagtg acctctgccc    240 

tgtgatggct caacaccatc acacgcaact gtccagacaa gccccctcaa cgggctgctg    300 

t                                                                    301 

 
           
             15  
             259  
             DNA  
             Homo sapien  
           
            15 

gtcttgaaag tatttattgt ttaataattc tttctcccct cagccccatc cggccactct     60 

ctctttctgc ttttctgatc atcctaaagg ctgaatacat cctcctcctg tgtggaggac    120 

acgaagcaat actaaaatca atacactcga tcaggtcttc atcagatacc acgtcactgt    180 

gggtagagtg ctaattttca acaaatgtgg tgttcttagg gccccacaag gtagtccttt    240 

ctcaaggtcg ctgggccac                                                 259 

 
           
             16  
             301  
             DNA  
             Homo sapien  
           
            16 

cgaggttgtt cacattttca aataaataat actccccgta agtaataact gcaaccaatc     60 

agtgttattc agtgctatgc ctccttgtaa tgggtagtta ttaattattt tcagagcttt    120 

ctggaaatac tgtcctaact ggctatgttt aggatctttg ttatctctga agacaaagaa    180 

agaactagga ctcttaattt tggggtgctt cttgactctt agttgggaaa ctgaaaatat    240 

ttccaacctt ttacccacgt caatggcata ttctgggaat caccaccacc accaccacta    300 

c                                                                    301 

 
           
             17  
             301  
             DNA  
             Homo sapien  
           
            17 

gcccgggcag gtctggggcc tagggtggct ctttgcaaag ctgaggggca agctaaggaa     60 

gccaggcagg tcaggggccc tttcggcctt ctcaagcctc cacctgagtt ctcgtcaatg    120 

ccagtctccc tggtatgatt ggggacatta tcagagaaac atctaatagc gcacatctgg    180 

gcacccacac tctgcttcag ttgcatccat cctcccaccc caaattcaac tcctgaccca    240 

atacaaaaga cttttttaac caggatttct tcttgcagga aagctgactt ggaaacacgg    300 

g                                                                    301 

 
           
             18  
             301  
             DNA  
             Homo sapien  
           
            18 

attacaggca cgtgccacca cacctagcta atttttgagc atggggctca aaggaactgc     60 

tctctggggc atgtcagatt tcggatttgg ggctgcacac tgatactctc taagtggtgg    120 

aggaacttca tcccactgaa attcctttgg catttggggt tttgtttttc tttttttcct    180 

tcttcatcct cctccttttt taaaagtcaa cgagagcctt cgctgactcc accgaagaag    240 

tgcaccactg gggaccaccc agtgccaggc gcccgtccag ggacacacac agtcttcact    300 

g                                                                    301 

 
           
             19  
             301  
             DNA  
             Homo sapien  
           
            19 

agaatctctg cactgtcatc aggtacaaca aaagatcaaa cccctgtccc gatgttaact     60 

ttttaactta aaagaatgcc agaaaaccca gatcaacact ttccagctac gagccgtcca    120 

caaaggccac ccaaaggcca gtcagactcg tgcagatctt attttttaat agtagtaacc    180 

acaatacaca gctctttaaa gctgttcata ttcttccccc attaaacacc tgccccgggc    240 

ggccaagggc gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag    300 

a                                                                    301 

 
           
             20  
             290  
             DNA  
             Homo sapien  
           
            20 

aggttttttt tttttttttt tttttttttt tttttccctt tcaattcatt taatttcaac     60 

aatctgtcaa aaaacagcca ataaacaaat actgaattac attctgctgg gttttttaaa    120 

ggctctaaac tataaaaaca tcttgtgtct cccaccctga ccaccctgct acttttccat    180 

ataccacagg ccacccataa acacaaagcc agggggtgaa gctgacatgg tctatttgga    240 

gccagtaaac aggagggcga taagtcctga taagcactta tggacaatat               290 

 
           
             21  
             301  
             DNA  
             Homo sapien  
           
            21 

agaaaggtaa ctgccagcca ggcttgcatt gtttagccag aaattgctgc ttggttctag     60 

actctttaaa aaaaaaaaat acccagggtt tgtcatcatt ttcagaggca gagtgccaaa    120 

tatcacccaa agctcttgtg tctttttttt acccccttat tttattttta tttattaatt    180 

ttttgtgcaa acatcaaatg tcactggtgt tcacagaagg cttttttgac tagccttaaa    240 

ttcctgagtc aaaagattaa tcagattttc aggcagtgtt taatcaggtg ctttgtcctg    300 

t                                                                    301 

 
           
             22  
             301  
             DNA  
             Homo sapien  
           
            22 

gacgccatgc accctccggt aaccagcagc cgcctgtcca tcccccaaga ccggaaaggc     60 

agcagcagcc cccgggagcc cagggctgtc ctcggtgcat ctggctgcag agggaaattg    120 

atgaccttac acagcaacta gcggccatgc agtccttcac tgacaagttc caggaccttt    180 

gaagttggag ccagcgtccg gagctgcagc caagcgagtt tcctccttat cctccttagc    240 

cagggctttt tctcttccgc tgcatttgcc cccttcccaa cgcaattcaa agcagttgtg    300 

a                                                                    301 

 
           
             23  
             381  
             DNA  
             Homo sapien  
           
            23 

cgaggtccag acagtggacc aagagatacg ctacataaat tggggtttca caattcttac     60 

attatttgtc tgtcacagaa gagagctgct tatgattttg aaggggtcag ggagggtggg    120 

agttggtaaa gagtagggta tttctataac agatattatt cagtcttatt tcctaagatt    180 

ttgttgtaac ttaaggtatc ttgctacagt agacagaatt ggtaatagca acttttaaaa    240 

ttgtcattag ttctgcaata ttagctgaaa tgtagtacag aaaagaatgt acatttagac    300 

atttgggttc agttgcttgt agtctgtaaa tttaaaacag cttaatttgg tacaggttac    360 

acatatggac ctcccgggcg g                                              381 

 
           
             24  
             214  
             DNA  
             Homo sapien  
           
            24 

aatgatgtaa aaattaatca acagggctgc cacttgcgaa tcccctccaa ggatgctgtg     60 

caaagggtct cattggtcct gatgaataat cttgtgactg tacatattcc tgggtgcatg    120 

tccacaaata ctgaggtata gcctgcatgc cactaaaaat aacaaaggtt tcaggggtgg    180 

aaacattgtc caccacactg tcatgaccat cttt                                214 

 
           
             25  
             302  
             DNA  
             Homo sapien  
           
            25 

gggggcactg agaactccct ctggaattct tggggggtgt tggggagaga ctgtgggcct     60 

ggagataaaa cttgtctcct ctaccaccac cctgtaccct agcctgcacc tgtcctcatc    120 

tctgcaaagt tcagcttcct tccccaggtc tctgtgcact ctgtcttgga tgctctgggg    180 

agctcatggg tggaggagtc tccaccagag ggaggctcag gggactggtt gggccaggga    240 

tgaatatttg agggataaaa attgtgtaag aagccaaaga aattggtagt aggggggaga    300 

ac                                                                   302 

 
           
             26  
             301  
             DNA  
             Homo sapien  
           
            26 

ttggagaacg cgctgacata ctgctcggcc acagtcagtg aagctgctgc atctccatta     60 

tgttgtgtca gagctgcagc caggattcga atagcttcag ctttagcctt ggccttcgcc    120 

agaactgcac tggcctctcc tgctgcctga tttatctgtg cagccttttc tgcttcggag    180 

gccaggatct gggcctgttt cttcccttct gccacattga tggccgactc tcgggtcccc    240 

tcagactcta gaactgtggc ccgtttccgc cgctctgcct ccacctgcat ctgcatagac    300 

t                                                                    301 

 
           
             27  
             301  
             DNA  
             Homo sapien  
           
            27 

aaatcagtca tcacatctgt gaaaagagtg ctagttataa caaatgagat cacaaatttg     60 

accattttat tagacaccct ctattagtgt taacagacaa agatgaaggt taagttgaaa    120 

tcaaattgaa atcatcttcc ctctgtacag attgcaatat ctgataatac cctcaacttt    180 

cttggtgcaa attaattgcc tggtactcac agtccagtgt taacaggcaa taatggtgtg    240 

attccagagg agaggactag gtggcaggaa aataaatgag attagcagta tttgacttgg    300 

a                                                                    301 

 
           
             28  
             286  
             DNA  
             Homo sapien  
           
            28 

tttttttttg cacaggatgc acttattcta ttcattctcc cccacccttc ccatatttac     60 

atccttagag gaagagaggg gtaaggtgat aaagtaactg aaggaccgca agacgggtat    120 

gtcccttgtt caccaaatgg tcaaagggtc aaagatcgga ggaggtcagg gggtaacgca    180 

ggaacaggtg agggcgtttc gccctctctc cctctcccct tttcaacctc ttaatcactg    240 

gctaactcgc gacctcatgg gttaattcgt aagcttacac gcgttg                   286 

 
           
             29  
             301  
             DNA  
             Homo sapien  
           
            29 

gtcatgttct tgctcttcct tctttacaca tttgagttgt gccttctgtt cttaaagaga     60 

ttttcctttg ttcaaaggat ttattcctac catttcacaa atccgaaaat aattgaggaa    120 

acaggttaca tcattccaat tttgccttgg gtttgaagag tctctcatgg tggcacagtc    180 

ctccagggta gctatgttgt tgggctcccc tacatcccag aagctcagag actttgtcaa    240 

aggtgtgccg tccacccatt gccactgacc ctcgacaacc tggtctgaca gtccaataaa    300 

a                                                                    301 

 
           
             30  
             332  
             DNA  
             Homo sapien  
           
            30 

gagcagaatt gatgcctatg gctccaagtc aaatactgct aatctcattt attttcctgc     60 

cacctagtcc tctcctctgg aatcacacca ttattgcctg ttaacactgg actgtgagta    120 

ccaggcaatt aatttgcacc aagaaagttg agggtattat cagatattgc aatctgtaca    180 

gagggaagat gatttcaatt tgatttcaac ttaaccttca tctttgtctg ttaacactaa    240 

tagagggtgt ctaataaaat ggtcaaattt gtgatctcat ttgttataac tagcactctt    300 

ttcacagatg tgatgactga tttccagcag ac                                  332 

 
           
             31  
             141  
             DNA  
             Homo sapien  
           
            31 

aaaggctatc aagtactttg aaggacagga aggaatgaac acacccaggt ggacgtttgg     60 

tttcatttgc aggggttcag ggagggttgc aggggttcag ggagggctct tgtcccacaa    120 

ccgggggaag ggagagggca c                                              141 

 
           
             32  
             201  
             DNA  
             Homo sapien  
           
            32 

gagctgatct cacagcacat acagaatgat gctactatgt agaccctcac tcccttggga     60 

aatctgtcat ctaccttaaa gagagaaaaa agatggaaca taggcccacc tagtttcatc    120 

catccaccta cataaccaac atagatgtga ggtccactgc actgatagcc agactgcctg    180 

gggtaaacct tttcagggag g                                              201 

 
           
             33  
             181  
             DNA  
             Homo sapien  
           
            33 

tttcaaaaca ctcatatgtt gcaaaaaaca catagaaaaa taaagtttgg tgggggtgct     60 

gactaaactt caagtcacag acttttatgt gacagattgg agcagggttt gttatgcatg    120 

tagagaaccc aaactaattt attaaacagg atagaaacag gctgtctggg tgaaatggtt    180 

c                                                                    181 

 
           
             34  
             151  
             DNA  
             Homo sapien  
           
            34 

atgtcctgca cagtatagct tggacctctg ggcctgaacc agggtgagca tcaaggcccc     60 

catttctcct caccacgggg tcgcttgtca gctccaagaa ccagtctggc cccactgaga    120 

acttttcagt cgagggcctg atgaatcttg g                                   151 

 
           
             35  
             291  
             DNA  
             Homo sapien  
           
            35 

tctttagggc aaaatcatgt ttctgtgtac ctagcaatgt gttcccattt tattaagaaa     60 

agctttaaca cgtgtaatct gcagtcctta acagtggcgt aattgtacgt acctgttgtg    120 

tttcagtttg tttttcacct ataatgaatt gtaaaaacaa acatacttgt ggggtctgat    180 

agcaaacata gaaatgatgt atattgtttt ttgttatcta tttattttca tcaatacagt    240 

attttgatgt attgcaaaaa tagataataa tttatataac aggttttctg t             291 

 
           
             36  
             201  
             DNA  
             Homo sapien  
           
            36 

ctgatacaat tataataacg gttccctgaa ccttttagag tgcaattaag aacaaaaact     60 

aaattttgtt tacatgaata tggaataaat acaataatca aaatatgact ctccctaaaa    120 

gtgaaacaca caagccaatc cggaactgct gtgcgaaaga taaaatcgag aaaggcaagg    180 

tttcggtagg aggacgcgat g                                              201 

 
           
             37  
             121  
             DNA  
             Homo sapien  
           
            37 

catcacactg gcggccgctc gagcatgcat ctagagggcc caattcgccc tataatgagt     60 

cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta    120 

c                                                                    121 

 
           
             38  
             200  
             DNA  
             Homo sapien  
           
            38 

aaacatgtat tactctatat ccccaagtcc tagagcatga cctgcatgtt ggagatgttg     60 

tacagcaatg tatttatcca gacatacata tatgatattt agagacacag tgattctttt    120 

gataacacca cacatagaac attataatta cacacaaatt tatggtaaaa gaattaatat    180 

gctgtctggt gctgctgtta                                                200 

 
           
             39  
             760  
             DNA  
             Homo sapien  
           
            39 

gcgtggtcgt cggccgaggt cctgggctag acctaatggt ttattattgg tggagagaaa     60 

gatctggaaa tacttgaggt tattacatac tagattagct tctaatgtga accatttttc    120 

ttttaacagt gatcaaatta ttatttcgaa gttaatcgtt cccttggtgg ctgcatacac    180 

atcgcattaa caaacatact gttgtatttt ttcccagttt tgtttggcta tgccaccaca    240 

gtcatcccca gggtctatac atactatgtt tcaactgtat tatttgccat ttttggcatt    300 

agaatgcttc gggaaggctt aaagatgagc cctgatgagg gtcaagagga actggaagaa    360 

gttcaagctg aattaaagaa gaaagatgaa gaagtaagcc atggcactgt tgatctggac    420 

caaaaaggca ctcaactagg aataaacact ctacagaggt ttctcagtgg ccccatctgt    480 

gtgatatgcg gggctacaca aaaatagctt cttttgcttt gttctgttct tatacctgtc    540 

tgtgatctga cttggggttg gtgtgaatgt agtagagaaa ggaagctgac agatgaatac    600 

tgaacacagg taatcagttt ccttaattag gttgattata agctcctgaa aagcaggaac    660 

tgtattttat aattttacct gtttctcccg tggtgtctag gatagtaagt gagcagagca    720 

gtaaatactg tttggtttgt tcagacctgc ccgggcggcc                          760 

 
           
             40  
             452  
             DNA  
             Homo sapien  
           
            40 

aatcactaaa gatattgact agagaatgct gtgtgctatt tcaattacat ttgtttttct     60 

tttattaaca ggaattttga ttcttcaagg aagtggctca atttcaattt caggtgacca    120 

ggtttatcgt gacttttcct tcttgtttac ttttcgctag gaaggggagt tgtaggggca    180 

gattcaggta ttggaatagg aaaattacgt ctaaaccatg gaaatcttgg aaatggaatt    240 

ggtggaagtg ggcgaaatgg atatgggtaa gggaacacaa aaaaccctga agctaattca    300 

tcgctgtcac tgatacttct tttttctcgt tcctggtctt gagagactgg gaaaccaaca    360 

gccactgcca agatggctgt gatcaggagg agaactttct tcatctcaaa cgtttcagtc    420 

agttctttct ctcacctcgg ccgcgaccac gc                                  452 

 
           
             41  
             676  
             DNA  
             Homo sapien  
           
            41 

aatctttgaa tgccaagtct cttctgtact ttcttttatt aacatcatag tctttgcatc     60 

aagatacata gcaatgatag caggtttctt tttaaagctt agtattaata ttaaatattt    120 

ttccccattt aaattttaca ttacttgcca agaaaaaaaa aaaattaaaa ctcaagttac    180 

ttgaagcctg gacacacttc catgattagc cgggctaggt aaaagttggt ggctttattc    240 

ttcctgctct ataagcagat ccaggcccta gaaagatggg accagggtat ataattgttt    300 

ttgaaaagtg tgctacaaaa atggatggcc tgttataagc caggatacaa agttaaggat    360 

gggggtaagg gagggacatt ttcttccaga agaaaagaca gaatttctga agagtcccag    420 

tccataattt tcccaaaatg gttggaggag agggtaaaat ctcaacatga gtttcaaagt    480 

actgtctctg tgaggggccg gtagatgcct tgctgaggag ggatggctaa tttggaccat    540 

gccccatccc cagctaggag aatggaaatg gaaactttaa ttgcccagtg ggtgtgaaag    600 

tgggctgaag cttggttggt actgaattct ctaagaggtt tcttctagaa acagacaact    660 

cagacctgcc cgggcg                                                    676 

 
           
             42  
             468  
             DNA  
             Homo sapien  
           
            42 

agcgtggtcg cggccgaggt ttggccggga gcctgatcac ctgccctgct gagtcccagg     60 

ctgagcctca gtctccctcc cttggggcct atgcagaggt ccacaacaca cagatttgag    120 

ctcagccctg gtgggcagag aggtagggat ggggctgtgg ggatagtgag gcatcgcaat    180 

gtaagactcg ggattagtac acacttgttg attaatggaa atgtttacag atccccaagc    240 

ctggcaaggg aatttcttca actccctgcc ccccagccct ccttatcaaa ggacaccatt    300 

ttggcaagct ctatgaccaa ggagccaaac atcctacaag acacagtgac catactaatt    360 

aaaaccccct gcaaagccca gcttgaaacc ttcacttagg aacgtaatcg tgtcccctat    420 

cctacttccc cttcctaatt ccacagacct gcccgggcgg ccgctcga                 468 

 
           
             43  
             408  
             DNA  
             Homo sapien  
           
            43 

atcatatcaa aacactatct tcccatctgt ttctcaatgc ctgctacttc ttgtagatat     60 

ttcatttcag gagagcagca gttaaacccg tggattttgt agttaggaac ctgggttcaa    120 

acctctttcc actaattggc tatgtctctg gacagttttt tttttttttt ttttttttaa    180 

accctttctg aactttcact ttctatggct acctcaaaga attgttgtga ggcttgagat    240 

aatgcatttg taaagggtct gccagatagg aagatgctag ttatggattt acaaggttgt    300 

taaggctgta agagtctaaa acctacagtg aatcacaatg catttacccc cactgacttg    360 

gacataagtg aaaactagcc cgaagtctct ttttcaaatt acttacag                 408 

 
           
             44  
             160  
             DNA  
             Homo sapien  
           
            44 

tggtcgcggc cgaggtcttg tgtgccctgt ggtccagggg accaagaaca acaagatcca     60 

ctctctgtgc tacaatgatt gcaccttctc acgcaacact ccaaccagga ctttcaacta    120 

caacttctcc gctttggcaa acaccgtcac tcttgctgga                          160 

 
           
             45  
             231  
             DNA  
             Homo sapien  
           
            45 

cgagcggccg cccgggcagg tctggggagg tgattccatc cagagtcata tctgttgtca     60 

ccccaataag tcgatcagca aggctgacag gctgtgagga aaccccggcc ttgtagcctg    120 

tcacctctgg ggggatgatg actgcctggc agacgtaggc tgtgatagat ttgggagaaa    180 

acctgactca ccctcaggaa tccggaggtc ggtgacattg tcggtgcaca c             231 

 
           
             46  
             371  
             DNA  
             Homo sapien  
           
            46 

cccgggcagg tctgtgtaac atgccaaggc tttgcacttt ctgcagagca gttttttatt     60 

ttccttatca ggtacaggtt ttggtttttc ttgactatct ctgatgaatt tttcatgagt    120 

ctgtatatgc agaatctttt ccctaaatac tgcttcgtcc catgtctgaa ggcgtaaaat    180 

aaagtcattc atcatttttt ctttgtacat gtttatttgt tctttttcaa ttacaccaag    240 

cattactagt cagaaggaag cacttgctac ctcttgctct tcctctgcct ctggtttgga    300 

tcattttgat gacattgccc acattactca tgaaggatga caagattgca ctgtgcaatg    360 

tcaattgcct t                                                         371 

 
           
             47  
             261  
             DNA  
             Homo sapien  
           
            47 

gccctgtttt tatacacttc acatttgcag aaatataatg atgccctcat tatcagtgag     60 

catgcacgaa tgaaagatgc tctggattac ttgaaagact tcttcagcaa tgtccgagca    120 

gcaggattcg atgagattga gcaagatctt actcagagat ttgaagaaaa gctgcaggaa    180 

ctagaaagtg tttccaggga tcccagcaat gagaatccta aacttgaaga cctctgcttc    240 

atcttacaag aagagtacca c                                              261 

 
           
             48  
             701  
             DNA  
             Homo sapien  
           
            48 

cgagcggccc ccgggcaggt ccaattagta caagtctcat gatataatca ctgcctgcat     60 

acatatgcac agatccagtt agtgagtttg tcaagcttaa tctaattggt taagtctcaa    120 

agagattatt attcttgatg tttgctttgt attggctaac aaatgtgcag aggtaataca    180 

tatgtgatgt ccgatgtctc tgtctttttt tttgtcttta aaaaataatt ggcagcaact    240 

gtatttgaat aaaatgattt cttagtatga ttgtaccgta atgaatgaaa gtggaacatg    300 

tttctttttg aaagggagag aattgaccat ttattattgt gatgtttaag ttataactta    360 

ttgagcactt ttagtagtga taactgtttt taaacttgcc taataccttt cttgggtatt    420 

gtttgtaatg tgacttattt aacccccttt tttgtttgtt taagttgctg ctttaggtta    480 

acagcgtgtt ttagaagatt taaatttttt tcctgtctgc acaattagtt attcagagca    540 

agagggcctg attttataga agccccttga aaagaggtcc agatgagagc agagatacag    600 

tgagaaatta tgtgatctgt gtgttgtggg aagagaattt tcaatatgta actacggagc    660 

tgtagtgcca ttagaaactg tgaatttcca aataaatttg a                        701 

 
           
             49  
             270  
             DNA  
             Homo sapien  
           
            49 

agcggccgcc cgggcaggtc tgatattagt agctttgcaa ccctgataga gtaaataaat     60 

tttatgggcg ggtgccaaat actgctgtga atctatttgt atagtatcca tgaatgaatt    120 

tatggaaata gatatttgtg cagctcaatt tatgcagaga ttaaatgaca tcataatact    180 

ggatgaaaac ttgcatagaa ttctgattaa atagtgggtc tgtttcacat gtgcagtttg    240 

aagtatttaa attaaccact cctttcacag                                     270 

 
           
             50  
             271  
             DNA  
             Homo sapien  
           
            50 

atgcatttat ccatatgaac ttgattattc tgaattactg actataaaaa ggctattgtg     60 

aaagatatca cactttgaaa cagcaaatga attttcaatt ttacatttaa ttataagacc    120 

acaataaaaa gttgaacatg cgcatatcta tgcatttcac agaagattag taaaactgat    180 

ggcaacttca gaattatttc atgaagggta caaacagtct ttaccacaat tttcccatgg    240 

tcttatcctt caaaataaaa ttccacacac t                                   271 

 
           
             51  
             241  
             DNA  
             Homo sapien  
           
            51 

tggtcgcggc cgaggtgtga ggagatgaac tttgtgttaa tggggggcac tttaaatcga     60 

aatggcttat ccccaccgcc atgtaagtta ccatgcctgt ctcctccctc ctacacattt    120 

ccagctcctg ctgcagttat tcctacagaa gctgccattt accagccctc tgtgattttg    180 

aatccacgag cactgcaggc cctccacagc gttactaccc agcaggcact cagctcttca    240 

t                                                                    241 

 
           
             52  
             271  
             DNA  
             Homo sapien  
           
            52 

tccaagactt aaaacttagg aaacacctat gatgccactt taactggaag taatggagac     60 

atctgattcc aaattcacat tttaaatgcc tatttgcaat cagcaaagag ccaggtatgc    120 

tgcatgctgc ttgctgtaag ttacgatttg gcttcactag ctcaaatttt ttcactccac    180 

caaaagataa ggcacaggcc cgtttgtcca atcaagtttg ctgaaaatac tgcagcctga    240 

gtgtagacaa acttcccctg aatttgctag a                                   271 

 
           
             53  
             493  
             DNA  
             Homo sapien  
           
            53 

ttagcgtggt cgcggtccga ggtctggcct gactagctca ctctgaagag tgtctttcac     60 

atggattaac caaaaaatgc attactgcct ttggcacact gtcttgaata ttctttctga    120 

caatgagaaa atatgattta atggagtcgt tcaataacct cacaatctcg ctgttccgag    180 

cagatagttt tcgtgccaac aggaactggc acatctagca ggttcacggc atgacctttt    240 

tgtggactgg ctggcataat tggaatgggt tttgattttt cttctgctaa taactcttca    300 

agcttttgaa gttttcaagc attcctctcc agttgcctgt ggttggttct tgaacaccat    360 

ctccaacccc accacctcca gatgcaacct tgtctcgtga tacagacctg cccgggcggc    420 

cctcaagggc gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag    480 

agggcccaat tcg                                                       493 

 
           
             54  
             321  
             DNA  
             Homo sapien  
           
            54 

cgtggtcgcg gccgaggtct gtttgcttgt tggtgtgagt ttttcttctg gagactttgt     60 

actgaatgtc aataaactct gtgattttgt taggaagtaa aactgggatc tatttagcca    120 

ctggtaagct tctgaggtga aggattcagg gacatctcgt ggaacaaaca ctccccactg    180 

gactttctct ctggagatac ccttttgaat atacaatggc cttggctcac taggtttaaa    240 

tacaaacaag tctgaaaccc actgaagact gagagattgc agcaatattc tctgaattag    300 

gatcgggttc cataactcta a                                              321 

 
           
             55  
             281  
             DNA  
             Homo sapien  
           
            55 

ttgcaaatga aactgtggat gtataataag aaaacacaag ggtttattct taacactaaa     60 

attaacatgc cacacgaaga ctgcattaca gctctctgtt tctgtaatgc agaaaaatct    120 

gaacagccca ccttggttac agctagcaaa gatggttact tcaaagtatg gatattaaca    180 

gatgactctg acatatacaa aaaagctgtt ggctggacct gtgactttgt tggtagttat    240 

cacaagtatc aagcaactaa ctgttgtttc tccgaagatg g                        281 

 
           
             56  
             612  
             DNA  
             Homo sapien  
           
            56 

gcgtggtcgc ggccgaggtc ctgtccgggg gcactgagaa ctccctctgg aattcttggg     60 

gggtgttggg gagagactgt gggcctggag ataaaacttg tctcctctac caccaccctg    120 

taccctagcc tgcacctgtc ctcatctctg caaagttcag cttccttccc caggtctctg    180 

tgccactctg tcttggatgc tctggggagc tcatgggtgg aggagtctcc accagaggga    240 

ggctcagggg actggttggg ccagggatga atatttgagg gataaaaatt gtgtaagagc    300 

caaagaattg gtagtagggg gagaacagag aggagctggg ctatgggaaa tgatttgaat    360 

aatggagctg ggaatatggc tggatatctg gtactaaaaa agggtcttta agaacctact    420 

tcctaatctc ttccccaatc caaaccatag ctgtctgtcc agtgctctct tcctgcctcc    480 

agctctgccc caggctcctc ctagactctg tccctgggct agggcagggg aggagggaga    540 

gcagggttgg gggagaggct gaggagagtg tgacatgtgg ggagaggacc agacctgccc    600 

gggcggccgt cg                                                        612 

 
           
             57  
             363  
             DNA  
             Homo sapien  
           
            57 

gtcgcggccg aggtcctgag cgtcacccta gttctgcccc tttttagctg tgtagacttg     60 

gacaagacat ttgacttccc tttctccttg tctataaaat gtggacagtg gacgtctgtc    120 

acccaagaga gttgtgggag acaagatcac agctatgagc acctcgcacg gtgtccagga    180 

tgcacagcac aatccatgat gcgttttctc cccttacgca ctttgaaacc catgctagaa    240 

aagtgaatac atctgactgt gctccactcc aacctccagc gtggatgtcc ctgtctgggc    300 

cctttttctg ttttttattc tatgttcagc accactggca ccaaatacat tttaattcac    360 

cga                                                                  363 

 
           
             58  
             750  
             DNA  
             Homo sapien  
           
            58 

cgtggtcgcg gccgaggtct aattccacct gactggcaga acctgcgccc ctcgcctaac     60 

ctgcgccctt ctcccaactc gcgtgcctca cagaacccag gtgctgcaca gccccgagat    120 

gtggcccttc ttcaggaaag agcaaataag ttggtccaag tacttgatgc ttaaggaata    180 

cacaaaggtg cccatcaagc gctcagaaat gctgagagat atcatccgtg aatacactga    240 

tgtttatcca gaaatcattg aacgtgcatg ctttgtccta gagaagaaat ttgggattca    300 

actgaaagaa attgacaaag aagaacacct gtatattctc atcagtaccc ccgagtccct    360 

ggctggcata ctgggaacga ccaaagacac acccaagctc ggtctcttct tggtgattct    420 

gggtgtcatc ttcatgaatg gcaaccgtgc cagtgaggct gtcttttggg aggcactacg    480 

caagatggga ctgcgtcctg gggtgagaca tcccctccct tggagatcta aggaaacttc    540 

tcacctatga gtttgtaaag cagaaatacc tggactacag acgagtgccc aacagcaacc    600 

ccccggagta tgagttcctc tggggcctcc gtccctacca tgagactagc aagatgaaaa    660 

tgctgagatt cattgcagag gttcagaaaa gagaccctcg tgactggact gcacagttca    720 

tggaggctgc agatgaggac ctgcccgggc                                     750 

 
           
             59  
             505  
             DNA  
             Homo sapien  
           
            59 

tggccgcccg ggcaggtcca gtctacaagc agagcactct catggggagc accagatgag     60 

ttccagccgc agttctttta taagctttaa gtgcctcatg aagacgcgag gatctcttcc    120 

aagtgcaacc tggtcacatc agggcacatt cagcagcaga agtctgtttc cagtatagtc    180 

cttggtatgg ctaaattcca ctgtcccttt ctcagcagtc aataatccat gataaattct    240 

gtacaacact gtagtcaata acagcagcac cagacagcat attaattctt ttaccataaa    300 

tttgtgtgta attataatgt tctatgtgtg gtgttatcaa aagaatcact gtgtctctaa    360 

atatcatata tgtatgtctg gataaataca ttgctgtaca acatctccaa catgcaggtc    420 

atgctctaag acttggggat atagagtaat acatgtttcg tggacctcgg ccgcgaccac    480 

gctaagggcg aattctgcag atatc                                          505 

 
           
             60  
             520  
             DNA  
             Homo sapien  
           
            60 

cgtggtcgcg gccgaggtcc tcaggacaag gaaacaggta tcagcatgat ggtagcagaa     60 

accttatcac caaggtgcag gagctgactt cttccaaaga gttgtggttc cgggcagcgg    120 

tcattgcctg cccttgctgg agggctgatt ttagtgttgc ttattatgtt ggccctgagg    180 

atgcttcgaa gtgaaaataa gaggctgcag gatcagcggc aacagatgct ctcccgtttg    240 

cactacagct ttcacggaca ccattccaaa aaggggcagg ttgcaaagtt agacttggaa    300 

tgcatggtgc cggtcagtgg gcacgagaac tgctgtctga cctgtgataa aatgagacaa    360 

gcagacctca gcaacgataa gatcctctcg cttgttcact ggggcatgta cagtgggcac    420 

gggaagctgg aattcgtatg acggagtctt atctgaacta cacttactga acagcttgaa    480 

ggacctgccc gggcggccgc tcgaaagggg cgaattctgc                          520 

 
           
             61  
             447  
             DNA  
             Homo sapien  
           
            61 

agagaggtgt ttttattctt tggggacaaa gccgggttct gtgggtgtag gattctccag     60 

gttctccagg ctgtagggcc cagaggctta atcagaattt tcagacaaaa ctggaacctt    120 

tcttttttcc cgttggttta tttgtagtcc ttgggcaaac caatgtcttt gttcgaaaga    180 

gggaaaataa tccaaacgtt tttcttttaa cttttttttt aggttcaggg gcacatgtgt    240 

aggcttgcta tataggtaaa ttgcatgtca ccagggtttg ttgtacagat tatttcatca    300 

tccagataaa aagcatagta ccagataggt agttttttga tcctcaccct ccttccatgc    360 

tccgacctca ggtaggcccc agtgtctgac ctgcccggcg gcccgctcga aagggccaat    420 

tctgcagata tccatcacac tggccgg                                        447 

 
           
             62  
             83  
             PRT  
             Homo sapien  
           
            62 

Lys Lys Val Leu Leu Leu Ile Thr Ala Ile Leu Ala Val Ala Val Gly 
 1               5                  10                  15 

Phe Pro Val Ser Gln Asp Gln Glu Arg Glu Lys Arg Ser Ile Ser Asp 
            20                  25                  30 

Ser Asp Glu Leu Ala Ser Gly Phe Phe Val Phe Pro Tyr Pro Tyr Pro 
        35                  40                  45 

Phe Arg Pro Leu Pro Pro Ile Pro Phe Pro Arg Phe Pro Trp Phe Arg 
    50                  55                  60 

Arg Asn Phe Pro Ile Pro Ile Pro Ser Ala Pro Thr Thr Pro Leu Pro 
65                  70                  75                  80 

Ser Glu Lys 

 
           
             63  
             683  
             DNA  
             Homo sapien  
           
            63 

acaaagattg gtagctttta tattttttta aaaatgctat actaagagaa aaaacaaaag     60 

accacaacaa tattccaaat tataggttga gagaatgtga ctatgaagaa agtattctaa    120 

ccaactaaaa aaaatattga aaccactttt gattgaagca aaatgaataa tgctagattt    180 

aaaaacagtg tgaaatcaca ctttggtctg taaacatatt tagctttgct tttcattcag    240 

atgtatacat aaacttattt aaaatgtcat ttaagtgaac cattccaagg cataataaaa    300 

aaagwggtag caaatgaaaa ttaaagcatt tattttggta gttcttcaat aatgatrcga    360 

gaaactgaat tccatccagt agaagcatct ccttttgggt aatctgaaca agtrccaacc    420 

cagatagcaa catccactaa tccagcacca attccttcac aaagtccttc cacagaagaa    480 

gtgcgatgaa tattaattgt tgaattcatt tcagggcttc cttggtccaa ataaattata    540 

gcttcaatgg gaagaggtcc tgaacattca gctccattga atgtgaaata ccaacgctga    600 

cagcatgcat ttctgcattt tagccgaagt gagccactga acaaaactct tagagcacta    660 

tttgaacgca tctttgtaaa tgt                                            683 

 
           
             64  
             749  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(749)  
               n = A,T,C or G  
             
           
            64 

ctgttcattt gtccgccagc tcctggactg gatgtgtgaa aggcatcaca tttccatttt     60 

cctccgtgta aatgttttat gtgttcgcct actgatccca ttcgttgctt ctattgtaaa    120 

tatttgtcat ttgtatttat tatctctgtg ttttccccct aaggcataaa atggtttact    180 

gtgttcattt gaacccattt actgatctct gttgtatatt tttcatgcca ctgctttgtt    240 

ttctcctcag aagtcgggta gatagcattt ctatcccatc cctcacgtta ttggaagcat    300 

gcaacagtat ttattgctca gggtcttctg cttaaaactg aggaaggtcc acattcctgc    360 

aagcattgat tgagacattt gcacaatcta aaatgtaagc aaagtaagtc attaaaaata    420 

caccctctac ttgggcttta tactgcatac aaatttactc atgagccttc ctttgaggaa    480 

ggatgtggat ctccaaataa agatttagtg tttattttga gctctgcatc ttancaagat    540 

gatctgaaca cctctccttt gtatcaataa atagccctgt tattctgaag tgagaggacc    600 

aagtatagta aaatgctgac atctaaaact aaataaatag aaaacaccag gccagaacta    660 

tagtcatact cacacaaagg gagaaattta aactcgaacc aagcaaaagg cttcacggaa    720 

atagcatgga aaaacaatgc ttccagtgg                                      749 

 
           
             65  
             612  
             DNA  
             Homo sapien  
           
            65 

acagcagcag tagatggctg caacaacctt cctcctaccc cagcccagaa aatatttctg     60 

ccccacccca ggatccggga ccaaaataaa gagcaagcag gcccccttca ctgaggtgct    120 

gggtagggct cagtgccaca ttactgtgct ttgagaaaga ggaaggggat ttgtttggca    180 

ctttaaaaat agaggagtaa gcaggactgg agaggccaga gaagatacca aaattggcag    240 

ggagagacca tttggcgcca gtcccctagg agatgggagg agggagatag gtatgagggt    300 

aggcgctaag aagagtagga ggggtccact ccaagtggca gggtgctgaa atgggctagg    360 

accaacagga cactgactct aggtttatga cctgtccata cccgttccac agcagctggg    420 

tgggagaaat caccattttg tgacttctaa taaaataatg ggtctaggca acagttttca    480 

atggatgcta aaacgattag gtgaaaagtt gatggagaat tttaattcag gggaattagg    540 

ctgataccat ctgaaaccat ttggcatcat taaaaatgtg acaacctggt ggctgccagg    600 

gaggaagggg ag                                                        612 

 
           
             66  
             703  
             DNA  
             Homo sapien  
           
            66 

tagcgtggtc gcggccgagg tacattgatg ggctggagag cagggttggc agcctgttct     60 

gcacagaacc aagaattaca gaaaaaagtc caggagctgg agaggcacaa catctccttg    120 

gtagctcagc tccgccagct gcagacgcta attgctcaaa cttccaacaa agctgcccag    180 

accagcactt gtgttttgat tcttcttttt tccctggctc tcatcatcct gcccagcttc    240 

agtccattcc agagtcgacc agaagctggg tctgaggatt accagcctca cggagtgact    300 

tccagaaata tcctgaccca caaggacgta acagaaaatc tggagaccca agtggtagag    360 

tccagactga gggagccacc tggagccaag gatgcaaatg gctcaacaag gacactgctt    420 

gagaagatgg gagggaagcc aagacccagt gggcgcatcc ggtccgtgct gcatgcagat    480 

gagatgtgag ctggaacaga ccttcctggc ccacttcctg atcacaagga atcctgggct    540 

tccttatggc tttgcttccc actgggattc ctacttaggt gtctgccctc aggggtccaa    600 

atcacttcag gacaccccaa gagatgtcct ttagtctctg cctgaggcct agtctgcatt    660 

tgtttgcata tatgagaggg tacctgcccg ggcggccgct cga                      703 

 
           
             67  
             1022  
             DNA  
             Homo sapien  
           
            67 

cttgagaaag caggattgtt ttaagttcca agatttaaca aacttactgt tcagcatcat     60 

attcaagcct aaaaggaaga taggattttc aagatatatt tccaacttct ttaacatggc    120 

accatggatg aactgtttct cagcactgtg ctgcttcact tggaattaag gatgaattgg    180 

gaggagacag tatgacatag gtgggtaggt tgggtggtga ggggaaccag ttctaatagt    240 

cctcaactcc actccagctg ttcctgttcc acacggtcca ctgagctggc ccagtccctt    300 

tcactcagtg tgtcaccaaa ggcagcttca aggctcaatg gcaagagacc acctataacc    360 

tcttcacctt ctgctgcctc tttctgctgc cactgactgc catggccatc tgctatagcc    420 

gcattgtcct cagtgtgtcc aggccccaga caaggaaggg gagccatggt gagactccaa    480 

ttcccaggcc ttaatcctta accctagacc tgttgcctct agcatcattt atttatctac    540 

ctacctaata gctatctacc agtcattaaa ccatggtgag attctaacca tgtctagcac    600 

ctgatgctag agataatttt gttgaatccc ttcaattata aacagctgag ttagctggac    660 

aaggactagg gaggcaatca gtattattta ttcttgaaca ccatcaagtc tagacttggt    720 

ggcttcatat ttctatcata atccctgggg gtaagaaatc atatagcccc aggttgggaa    780 

ggggaaaacg gtttgcaaca ttctcctcct tgtaggaggc gagctctgtc tcactagcta    840 

tgcccctcca tcaattcacc ctatactcag atcagaagct gagtgtctga attacagtat    900 

attttctaaa ttcctagccc ctgctggtga atttgccctc ccccgctcct ttgacaattg    960 

tccccgtgtt cgtctccggg ccctgagact ggccctgctt atcttgctga ccttcatcct   1020 

ct                                                                  1022 

 
           
             68  
             449  
             DNA  
             Homo sapien  
           
            68 

ccagatccat tttcagtggt ctggatttct ttttattttc ttttcaactt gaaagaaact     60 

ggacattagg ccactatgtg ttgttactgc cactagtgtt caagtgcctc ttgttttccc    120 

agagatttcc tgggtctgcc agaggcccag acaggctcac tcaagctctt taactgaaaa    180 

gcaacaagcc actccaggac aaggttcaaa atggttacaa cagcctctac ctgtcgcccc    240 

agggagaaag gggtagtgat acaagtctca tagccagaga tggttttcca ctccttctag    300 

atattcccaa aaagaggctg agacaggagg ttattttcaa ttttattttg gaattaaata    360 

cttttttccc tttattactg ttgtagtccc tcacttggat atacctctgt tttcacgata    420 

gaaataaggg aggtctagag cttctattc                                      449 

 
           
             69  
             387  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(387)  
               n = A,T,C or G  
             
           
            69 

gcccttagcg tgggtcgcgg cncgangtct ggagcntatg tgatncctat ggtncncagg     60 

cnnatactgc tantctcatt tattctcctg cnacctantc ctctnctctg gaatcacacc    120 

attattgcct gttaacactg gactgtgagt accangcaat taatttgcac caanaaagtt    180 

gagggtatta tcanatattg caatctgtac agagggaaga tgatttcaat ttgatttcaa    240 

cttaaccttc atctttgtct gttaacacta atagagggtg tctaataaaa tggcaaattt    300 

gngatctcat tnggtataac tacactcttt ttcacagatg tgatgactga atttccanca    360 

acctgcccgg gcggncgntc naagggc                                        387 

 
           
             70  
             836  
             DNA  
             Homo sapien  
           
            70 

tattccattt acaaaataaa ttcagccctg cactttcttt agatgccttg atttccagaa     60 

tggagcttag tgctactgaa taccctggcc acagagccac ctcaggatat tcttttctcc    120 

accctagttt atttatttat agatatctgt ttacaaagtc tgtagtaaat cctgatgctg    180 

accatctgaa atgtactttt tttctgaatg ctgtttcaat ctaaaatagc agcttttgag    240 

aaaacaatga tgtaaattcc ttatgataaa aggatgattc tatatattct ttaatgatat    300 

taaatatgcc gaagccaagc acacagtctt tctaaagtgt gtgtatgttt gtgtgaatgt    360 

gaatgatact gatcttatat ctgttaaaag ttgttttaaa aagctgtggc atcccattgt    420 

tcatatttgc caagtcttct gtaaagatgt ctaggacgaa atattttatg tgctaatgca    480 

tgtatttgta aaccagattt gtttaccact caaaattaac ttgttttctt catccaaaaa    540 

agtttatttc ttccacgtac ttaaattttc tgtgtgggta taatatagct ttctaatttt    600 

tttctttcac aaaggcaggt tcaaaattct gttgaaagaa aaatgctttc tgaaactgag    660 

gtataacacc agagcttgct gtttaaagga ttatatgatg tacatcagtt ctataaatgt    720 

gctcagcagt ttaacatgtg aatcctgttt taaagtgctc agatttcaac tgtgtaagcc    780 

attgatataa cgctgtaatt aaaaatgttt atatgaaaaa aaaaaaaaaa aaaaaa        836 

 
           
             71  
             618  
             DNA  
             Homo sapien  
           
            71 

gttgcagtga gctcaagtgt tgggtgtatc agctcaaaac accatgtgat gccaatcatc     60 

tccacaggag caatttgttt accttttttt tctgatgctt tactaacttc atcttttaga    120 

tttaaatcat tagtagatcc tagaggagcc agtttcagaa aatatagatt ctagttcagc    180 

accacccgta gttgtgcatt gaaataatta tcattatgat tatgtatcag agcttctggt    240 

tttctcattc tttattcatt tattcaacaa ccacgtgaca aacactggaa ttacaggatg    300 

aagatgagat aatccgctcc ttggcagtgt tatactatta tataacctga aaaaacaaac    360 

aggtaatttt cacacaaagt aatagatatc atgacacatt taaaataggg cactactgga    420 

acacacagat aggacatcca ggttttgggt caatattgta gactttttgg tggatgagat    480 

atgcaggttg atrccagaag gacaacaaaa acatatgtca gatagaaggg aggagcaaat    540 

gccaagagct ggagctgagg aagatcactg tgaaattcta tgtagtctag ttggctggat    600 

gctagagcaa agaggtgg                                                  618 

 
           
             72  
             806  
             DNA  
             Homo sapien  
           
            72 

tctacgatgg ccatttgctc attgtctttc ctctgtgtgt agtgagtgac cctggcagtg     60 

tttgcctgct cagagtggcc cctcagaaca acagggctgg ccttggaaaa accccaaaac    120 

aggactgtgg tgacaactct ggtcaggtgt gatttgacat gagggccgga ggcggttgct    180 

gacggcagga ctggagaggc tgcgtgcccg gcactggcag cgaggctcgt gtgtccccca    240 

ggcagatctg ggcactttcc caacccaggt ttatgccgtc tccagggaag cctcggtgcc    300 

agagtggtgg gcagatctga ccatccccac agaccagaaa caaggaattt ctgggattac    360 

ccagtccccc ttcaacccag ttgatgtaac cacctcattt tttacaaata cagaatctat    420 

tctactcagg ctatgggcct cgtcctcact cagttattgc gagtgttgct gtccgcatgc    480 

tccgggcccc acgtggctcc tgtgctctag atcatggtga ctcccccgcc ctgtggttgg    540 

aatcgatgcc acggattgca ggccaaattt cagatcgtgt ttccaaacac ccttgctgtg    600 

ccctttaatg ggattgaaag cacttttacc acatggagaa atatattttt aatttgtgat    660 

gcttttctac aaggtccact atttctgagt ttaatgtgtt tccaacactt aaggagactc    720 

taatgaaagc tgatgaattt tcttttctgt ccaaacaagt aaaataaaaa taaaagtcta    780 

tttagatgtt gaaaaaaaaa aaaaaa                                         806 

 
           
             73  
             301  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(301)  
               n = A,T,C or G  
             
           
            73 

actctggtaa gcttgttgtt gtccaagtga agctccctca gatgaggcgt gttggccana     60 

gagccattgt caacagcaga gatgctgttg aaactcaatc ccaacttagc caaattattc    120 

agtcctttca ggctagctgc atcaactctg ctgattttgt tgccatcaag atgtaattcc    180 

gtaagggaag gaggaagacc ttgaggaatg ctggygatat tggyatcagc aatgcggatg    240 

tasgaagagc ttcttcmttc cctggaaagc cccattttca atyccttgag ctcttcakcg    300 

g                                                                    301 

 
           
             74  
             401  
             DNA  
             Homo sapien  
           
            74 

agtttacatg atccctgtaa cagccatggt ctcaaactca gatgcttcct ccatctgcca     60 

agtgtgttct ggatacagag cacatcgtgg cttctggggt cacactcagc ttaggctgtg    120 

ggtccacaga gcactcatct ggctgggcta tggtggtggt ggctctactc aagaagcaaa    180 

gcagttacca gcacattcaa acagtgtatt gaacatcttt taaatatcaa agtgagaaac    240 

aagaaggcaa cataataatg ttatcagaaa gatgttagga agtaaggaca gctgtgtaaa    300 

gcttgaggct gaaaagtagc ttgccagctt catttctttg gtttcttggg tagtgggccg    360 

ccggaacagc aagatgtgag gttctggttc atggatcata t                        401 

 
           
             75  
             612  
             DNA  
             Homo sapien  
           
            75 

ttatttttca atttttattt tggttttctt acaaaggttg acattttcca taacaggtgt     60 

aagagtgttg aaaaaaaaat tcaaattttt ggggagcgag ggaaggagtt aatgaaactg    120 

tattgcacaa tgctctgatc aatccttctt tttctctttt gcccacaatt taagcaagta    180 

gatgtgcaga agaaatggaa ggattcagct ttcagttaaa aaagaagaag aagaaatggc    240 

aaagagaaag ttttttcaaa tttctttctt ttttaattta gattgagttc atttatttga    300 

aacagactgg gccaatgtcc acaaagaatt cctggtcagc accaccgatg tccaaaggtg    360 

caatatcaag gaagggcagg cgtgatggct tatttgtttt gtattcaatg attgtctttc    420 

cccattcatt tgtcttttta gagcagccat ctacaagaac agtgtaagtg aacctgctgt    480 

tgccctcagc aacaagttca acatcattag agccctgtag aatgacagcc tttttcaggt    540 

tgccagtctc ctcatccatg tatgcaatgc tgttcttgca gtggtaggtg atgttctgag    600 

aggcatagtt gg                                                        612 

 
           
             76  
             844  
             DNA  
             Homo sapien  
           
            76 

ggctttcgag cggccgcccg ggcaggtctg atggttctcg taaaaacccc gctagaaact     60 

gcagagacct gaaattctgc catcctgaac tcaagagtgg agaatactgg gttgacccta    120 

accaaggatg caaattggat gctatcaagg tattctgtaa tatggaaact ggggaaacat    180 

gcataagtgc caatcctttg aatgttccac ggaaacactg gtggacagat tctagtgctg    240 

agaagaaaca cgtttggttt ggagagtcca tggatggtgg ttttcagttt agctacggca    300 

atcctgaact tcctgaagat gtccttgatg tgcagcykgc attccttcga cttctctcca    360 

gccgagcttc ccagaacatc acatatcact gcaaaaatag cattgcatac atggatcagg    420 

ccagtggaaa tgtaaagaag gccctgaagc tgatggggtc aaatgaaggt gaattcaagg    480 

ctgaaggaaa tagcaaattc acctacacag ttctggagga tggttgcacg aaacacactg    540 

gggaatggag caaaacagtc tttgaatatc gaacacgcaa tgctgttcct tgacattgca    600 

ccaccaatgt ccagaggtgc aatgtcaagg aacggcaggc gagatggctt atttgttttg    660 

tattcaatga ttgtcttgcc ccattcattt gtctttttgg agcagccatc gactaggaca    720 

gagtaggtga acctgctgtt gccctcagca acaagttcca catcgttgga accctgcaga    780 

agcacagcct tgttcaarct gcccgtctcc tcatccagat acctcggccg cgaccacgct    840 

aatc                                                                 844 

 
           
             77  
             314  
             DNA  
             Homo sapien  
           
            77 

ccagtcctcc acttggcctg atgagagtgg ggagtggcaa gggacgtttc tcctgcaata     60 

gacacttaga tttctctctt gtgggaagaa accacctgtc catccactga ctcttctaca    120 

ttgatgtgga aattgctgct gctaccacca cctcctgaag aggcttccct gatgccaatg    180 

ccagccatcc tggcatcctg gccctcgagc aggctgcggt aagtagcgat ctcctgctcc    240 

agccgtgtct ttatgtcaag cagcatcttg tactcctggt tctgagcctc catctcgcat    300 

cggagctcac tcag                                                      314 

 
           
             78  
             548  
             DNA  
             Homo sapien  
           
            78 

accaagagcc aagtgttaca caggatattt taaaaataaa atgtttttgg aatcctcacc     60 

tcccatgcta tcttctaaga taactacaaa tattcttcaa agatttaact gagttctgcc    120 

aaggacctcc caggactcta tccagaatga ttattgtaaa gctttacaaa tcccaccttg    180 

gccctagcga taattaggaa atcacaggca aacctcctct ctcggagacc aatgaccagg    240 

ccaatcagtc tgcacattgg ttttgttaga tactttgtgg agaaaaacaa aggctcgtga    300 

tagtgcagct ctgtgcctac agagagcctc ccttttggtt ctgaaattgc tgatgtgaca    360 

gagacaaagc tgctatgggt ctaaaacctt caataaagta actaatgaca ctcaaggtcc    420 

tgggactctg agacagacgg tggtaaaacc cacagctgcg attcacattt ccaatttatt    480 

ttgagctctt tctgaagctg ttgcttccta cctgagaatt cccatttaga gagctgcaca    540 

gcacagtc                                                             548 

 
           
             79  
             646  
             DNA  
             Homo sapien  
           
            79 

accccgtcac tatgtgaata aaggcagcta gaaaatggac tcaattctgc aagccttcat     60 

ggcaacagcc catattaaga cttctagaac aagttaaaaa aaatcttcca tttccatcca    120 

tgcatgggaa aagggcttta gtatagttta ggatggatgt gtgtataata ataaaatgat    180 

aagatatgca tagtggggga ataaagcctc agagtccttc cagtatgggg aatccattgt    240 

atcttagaac cgagggattt gtttagattg ttgatctact aatttttttc ttcacttata    300 

tttgaatttt caatgatagg acttattgga aattggggat aattctgttg tggtattaaa    360 

taatattcat tttttaaaaa ctcatcttgg tattgagtta gtgcattgac ttccaatgaa    420 

ttgacataag cccatatttc attttaacca gaaacaaaaa ctagaaaatg ttactcccta    480 

aataggcaac aatgtatttt ataagcactg cagagattta gtaaaaaaca tgtatagtta    540 

ctttagaaac aacttctgac acttgagggt tacccaatgg tctccttccc attctttata    600 

tgaggtaaat gcaaaccagg gagccaccga ataaacagcc ctgagt                   646 

 
           
             80  
             276  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(276)  
               n = A,T,C or G  
             
           
            80 

gtctgaatga gcttcnctgc gagatgganc ancataaccc agaantccaa aancntanng     60 

aacgnnaaaa cccgntngaa caagnaaacn gcaactnacg gccgcctgnt gnagggcgag    120 

gacgcccacc tctcctcctc ccagttctcc tctggatcgc agncatccan agatgtgacc    180 

tcttccagcc gccaaatccg caccaaggtc atggatgtgc acgatggcaa ggtgggtgtc    240 

cacccacgaa caggtccttc gcaccaagaa ctgagg                              276 

 
           
             81  
             647  
             DNA  
             Homo sapien  
           
            81 

gtcctgcctt tcatcttttc tttaaaaaaa ataaatgttt acaaaacatt tccctcagat     60 

tttaaaattc atggaagtaa taaacagtaa taaaatatgg atactatgaa aactgacaca    120 

cagaaaaaca taaccataaa atattgttcc aggatacaga tattaattaa gagtgacttc    180 

gttagcaaca cgtagacatt catacatatc cggtggaaga ctggtttctg agatgcgatt    240 

gccatccaaa cgcaaatgct tgatcttgga gtaggrtaat ggccccagga tcttgcagaa    300 

gctctttatg tcaaacttct caagttgatt gacctccagg taatagtttt caaggttttc    360 

attgacagtt ggtatgtttt taagcttgtt ataggacaga tccagctcaa ccagggatga    420 

cacattgaaa gaatttccag gtattccact atcagccagt tcgttgtgag ataaacgcag    480 

atactgcaat gcattaaaac gcttgaaata ctcatcaggg atgttgctga tcttattgtt    540 

gtctaagtag agagttagaa gagagacagg gagaccagaa ggcagtctgg ctatctgatt    600 

gaagctcaag tcaaggtatt cgagtgattt aagaccttta aaagcag                  647 

 
           
             82  
             878  
             DNA  
             Homo sapien  
           
            82 

ccttctttcc ccactcaatt cttcctgccc tgttattaat taagatatct tcagcttgta     60 

gtcagacaca atcagaatya cagaaaaatc ctgcctaagg caaagaaata taagacaaga    120 

ctatgatatc aatgaatgtg ggttaagtaa tagatttcca gctaaattgg tctaaaaaag    180 

aatattaagt gtggacagac ctatttcaaa ggagcttaat tgatctcact tgttttagtt    240 

ctgatccagg gagatcaccc ctctaattat ttctgaactt ggttaataaa agtttataag    300 

atttttatga agcagccact gtatgatatt ttaagcaaat atgttattta aaatattgat    360 

ccttcccttg gaccaccttc atgttagttg ggtattataa ataagagata caaccatgaa    420 

tatattatgt ttatacaaaa tcaatctgaa cacaattcat aaagatttct cttttatacc    480 

ttcctcactg gccccctcca cctgcccata gtcaccaaat tctgttttaa atcaatgacc    540 

taagatcaac aatgaagtat tttataaatg tatttatgct gctagactgt gggtcaaatg    600 

tttccatttt caaattattt agaattctta tgagtttaaa atttgtaaat ttctaaatcc    660 

aatcatgtaa aatgaaactg ttgctccatt ggagtagtct cccacctaaa tatcaagatg    720 

gctatatgct aaaaagagaa aatatggtca agtctaaaat ggctaattgt cctatgatgc    780 

tattatcata gactaatgac atttatcttc aaaacaccaa attgtcttta gaaaaattaa    840 

tgtgattaca ggtagagaac ctcggccgcg accacgct                            878 

 
           
             83  
             645  
             DNA  
             Homo sapien  
           
            83 

acaaacattt tacaaaaaag aacattacca atatcagtgg cagtaagggc aagctgaaga     60 

ataaatagac tgagtttccg ggcaatgtct gtcctcaaag acatccaaac tgcgttcagg    120 

cagctgaaac aggcttcttt cccagtgaca agcatatgtg gtcagtaata caaacgatgg    180 

taaatgaggc tactacatag gcccagttaa caaactcctc ttctcctcgg gtaggccatg    240 

atacaagtgg aactcatcaa ataatttaaa cccaaggcga taacaacgct atttcccatc    300 

taaactcatt taagccttca caatgtcgca atggattcag ttacttgcaa acgatcccgg    360 

gttgtcatac agatacttgt ttttacacat aacgctgtgc catcccttcc ttcactgccc    420 

cagtcaggtt tcctgttgtt ggaccgaaag gggatacatt ttagaaatgc ttccctcaag    480 

acagaagtga gaaagaaagg agaccctgag gccaggatct attaaacctg gtgtgtgcgc    540 

aaaagggagg gggaaggcag gaatttgaaa ggataaacgt ctcctttgcg ccgaggaatc    600 

aggaagcgtg actcacttgg gtctgggacg ataccgaaat ccggt                    645 

 
           
             84  
             301  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(301)  
               n = A,T,C or G  
             
           
            84 

tctgatgtca atcacaactt gaaggatgcc aatgatgtac caatccaatg tgaaatctct     60 

cctcttatct cctatgctgg agaaggatta gaaggttatg tggcagataa agaattccat    120 

gcacctctaa tcatcgatga gaatggagtt catgggctgg tgaaaaatgg tatttgaacc    180 

agataccaag ttttgtttgc cacgatagga atagctttta tttttgatag accaactgtg    240 

aacctacaag acgtcttgga caactgaagn ttaaatatcc acangggttt attttgcttg    300 

g                                                                    301 

 
           
             85  
             296  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(296)  
               n = A,T,C or G  
             
           
            85 

agcgtgggtc gcggcncgan gtagagaacc gactgaaacg tttgagatga agaaagttct     60 

cctcctgatc acagccatct tggcagtggc tgttggtttc ccagtctctc aagaccagga    120 

acgagaaaaa agaagtatca gtgacagcga tgaattagct tcagggtttt ttgtgttccc    180 

ttacccatat ccatttcgcc cacttccacc aattccattt ccaagatttc catggtttan    240 

acgtaatttt cctattccaa tacctgaatc tgcccctaca actccccttc ctagcg        296 

 
           
             86  
             806  
             DNA  
             Homo sapien  
           
            86 

tctacgatgg ccatttgctc attgtctttc ctctgtgtgt agtgagtgac cctggcagtg     60 

tttgcctgct cagagtggcc cctcagaaca acagggctgg ccttggaaaa accccaaaac    120 

aggactgtgg tgacaactct ggtcaggtgt gatttgacat gagggccgga ggcggttgct    180 

gacggcagga ctggagaggc tgcgtgcccg gcactggcag cgaggctcgt gtgtccccca    240 

ggcagatctg ggcactttcc caacccaggt ttatgccgtc tccagggaag cctcggtgcc    300 

agagtggtgg gcagatctga ccatccccac agaccagaaa caaggaattt ctgggattac    360 

ccagtccccc ttcaacccag ttgatgtaac cacctcattt tttacaaata cagaatctat    420 

tctactcagg ctatgggcct cgtcctcact cagttattgc gagtgttgct gtccgcatgc    480 

tccgggcccc acgtggctcc tgtgctctag atcatggtga ctcccccgcc ctgtggttgg    540 

aatcgatgcc acggattgca ggccaaattt cagatcgtgt ttccaaacac ccttgctgtg    600 

ccctttaatg ggattgaaag cacttttacc acatggagaa atatattttt aatttgtgat    660 

gcttttctac aaggtccact atttctgagt ttaatgtgtt tccaacactt aaggagactc    720 

taatgaaagc tgatgaattt tcttttctgt ccaaacaagt aaaataaaaa taaaagtcta    780 

tttagatgtt gaaaaaaaaa aaaaaa                                         806 

 
           
             87  
             620  
             DNA  
             Homo sapien  
           
            87 

tttttgcatc agatctgaaa tgtctgagag taatagtttc tgttgaattt ttttttgttc     60 

atttttctgc acagtccatt ctgtttttat tactatctag gcttgaaata tatagtttga    120 

aattatgaca tccttcctct ttgttatttt cctcatgatt gctttggcta ttcaaagttt    180 

attttagttt catgtaaatt tttgaattgt attttccatt attgtgaaaa tagtaccact    240 

gcaattttaa taggaagttt attgaatcta tagattactt tggataatat ggcacttcaa    300 

taatattcat gttttcaatt catagacaaa atattttaaa atttatttgt atcttttcta    360 

atttttcctt tttttattgt aaagatttac ctccttggtt aatattttcc tcagaaattt    420 

attatttaag gtatagtcaa taaaattttc ttcctctatt ttgtcagata gtttaagtgt    480 

atgaaaccat agatatactt gtatgttaat tttatatttt gctaatttac tgagtgtatt    540 

tattagttta gagaggtttt aatgtactgt ttatggtttt ttaaatataa gattacttat    600 

tttttaaaaa aaaaaaaaaa                                                620 

 
           
             88  
             308  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(308)  
               n = A,T,C or G  
             
           
            88 

tagctgtgnt cagcaggccg aggttttttt tttttttgag atggagtctc gccctgtcac     60 

ccaggctgga gtgcagtggc ctgatctcag ctcactgcaa gctccacctc ctggattcac    120 

gctattctcc tgcctcagcc tcccaagtag ctgggactac aggcgcccgc caccacgccc    180 

agctaattnt ttgnattttt agtacnagat gcggtttcat cgtgttagcc agcatggnct    240 

cgatctcctg acctcgtgaa ctgcccgcct cggcctccca aagacctgcc cgggcnggcc    300 

gctcgaaa                                                             308 

 
           
             89  
             492  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(492)  
               n = A,T,C or G  
             
           
            89 

agcggccgcc cgggcaggtc tgttaagtaa catacatatc accttaataa aaatcaagat     60 

gaaatgtttt agaaactatt ttatcaaaag tggctctgat acaaagactt gtacatgatt    120 

gttcacagca gcactattaa tgccaaaaag tagacaaaac ctaaatgtcc attaactgat    180 

aagcaaaatg tggtatatcc atacaatgga atattatgta gcccacaaca tggcatggag    240 

tactacaaca tggatgagcc tcaaaaacgt tatgctaaat gaaaaaagtc agatatagga    300 

aaccacatgt catatgatcc catttatatg aaatagccag aaaaggcaag tcatagaaac    360 

aagatagatc ggaaaatggg ttggaggact acaaatggca ccagggatct ttgaagttga    420 

tggaaatggt ctaaaatcag actgtggntg tggttgaaca agtctgtaaa tttaccaaaa    480 

tgcgttaata ca                                                        492 

 
           
             90  
             390  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(390)  
               n = A,T,C or G  
             
           
            90 

tcgagcggcc gcccgggcag gtacaagctt tttttttttt tttttttttt ttttctaaca     60 

gttctctgtt ttattgcaat acagcaaagt ctggttaata ttaagngata tcaacataaa    120 

gtattggtga ggagtctttt gtgacatttt ttaccatccc accttaaata tttctgtgca    180 

aaanaatcca catcattgtt tggtancana ggatctctta aaaagttccc taanacactg    240 

agggcataaa accaaacaaa ataaaataag gagtgatagg ctaaagcagt atcttcccct    300 

ccatccacat ttgncaagca ttatattcta accaaaaaat gatcacacca ggccatgcaa    360 

aactgtccaa tattaccgag aaaaaaccct                                     390 

 
           
             91  
             192  
             DNA  
             Homo sapien  
           
            91 

agcgtggtcg cggccgaggt ctgtcaatta atgctagtcc tcaggattta aaaaataatc     60 

ttaactcaaa gtccaatgca aaaacattaa gttggtaatt actcttgatc ttgaattact    120 

tccgttacga aagtccttca catttttcaa actaagctac tatatttaag gcctgcccgg    180 

gcggccgctc ga                                                        192 

 
           
             92  
             570  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(570)  
               n = A,T,C or G  
             
           
            92 

agcgtggtcg cggccgaggt ctgacaacta acaaagaagc aaaaactggc atcttggaca     60 

tcctagtatt acacttgcaa gcaattagaa cacaaggagg gccaaggaaa aagtttagct    120 

ttgaatcact tccaaatcta ctgattttga ggttccgcag tagttctaac aaaacttttc    180 

agacaatgtt aactttcgat taagaaagaa aaaaacccca aacatcttca ggaattccat    240 

gccaggttca gtctcttcca gtgagcccgc ttgctaaaag tccacgtgca ccattaatta    300 

gctgggctgg cagcaccatg taaaaagaag cctattcacc accaaccaca cagactagac    360 

atgtaaagta ggatcaagta atggatgaca accatggtcg tggaatatgg tcaatgagag    420 

tcagaaaagt acaggcacca gtacaagcag cagataacag aattgacggg ccaaaggata    480 

aaaataggct tatttaaata ggatgctaca gaacacatnc acttctaatt ggaagctgct    540 

ttacactggg tggcattgna ccatatgcat                                     570 

 
           
             93  
             446  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(446)  
               n = A,T,C or G  
             
           
            93 

tcgagcggcc gcccgggcag gtccaggttt ttatttagtt gtgtaatctt ggacaagtta     60 

cctaactttt ttgagtctga atatatttaa tctgcaaaat gagaatcatg ataatacgtc    120 

ataggcttaa ttaggaggat taaatgaaat aatttatagg tggtgccatg gttacataca    180 

agtattagta gttaattctt ttcctttgtt tacttttata gtataggttg gatgaaggtt    240 

ccagtatagg caaaaatact acttgggggt aaagtagagt gtgatacttt atttgaaatg    300 

ttccctgaat ctgatcttta ctttttgnta ctgctgcact acccaaatcc aaattttcat    360 

cccaacattc ttggatttgt gggacagcng tagcagcttt tccaatataa tctatactac    420 

atcttttctt actttggtgc tttttg                                         446 

 
           
             94  
             409  
             DNA  
             Homo sapien  
           
            94 

cgagcggccg cccgggcagg tccatcagct cttctgctta gaatacgagg cagacagtgg     60 

agaggtcaca tcagttatcg tctatcaggg tgatgaccca agaaaggtga gtgagaaggt    120 

gtcggcacac acgcctctgg atccacccat gcgagaagcc ctcaagttgc gtatccagga    180 

ggagattgca aagcgccaga gccaacactg accatgttga aggcgttctc tccaggctgg    240 

attcactgca ctcggaagaa ttctgcccag ggaatttagt gtgggggtac caggaccagt    300 

ttgtcttgat cttgagaccc ccagagctgc tgcatccata gggtgttgca ggactacacc    360 

tggcctgcct tgcagtcatt ctttcttata tgttgaccca tttgcccaa                409 

 
           
             95  
             490  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(490)  
               n = A,T,C or G  
             
           
            95 

tcgagcggcc gcccgggcag gtcctacttg tttgcagctt ccacacactg cacctaccta     60 

ctacctctct tccatgctta actgggttta gaaaggtgag ctatgcgtag aagaactact    120 

tgggatattc aagtgctgta tttgaacgat aagcctatag ataacagtct gaagctgcaa    180 

gggagacttt gttagtacac tactataaac aggtaaacta cctgtttgta cttgatatag    240 

tgcatatgaa atgactgatt taatacaaaa ctacagaaca tgcaaaattt tttctgagat    300 

gttaagtatt acttcagtgg agaacaaaac ttacttaacc tttcgctaat gcatgtagta    360 

ccagaaagca aacatggttt tagcttcctt tactcaaaat atgaacatta agtggttgtg    420 

aattttgtct gccaagtggt tcagaaaata cattataaat aacctaagtt aaaaaaaaga    480 

aactgngaac                                                           490 

 
           
             96  
             223  
             DNA  
             Homo sapien  
           
            96 

agcgtggtcg cggccgaggt ctggaagccc accctaggac ttgaatggca ccttgtcctt     60 

tctctgccag taatgcaatc caacacaata tgctacaggg aaaacagaat ttccacggtg    120 

ccgccctctg gtacaaggga aacagcacgc aaagcaaaag gccacagagg gctccctgag    180 

aatccagtac aactaagcga ggacctgccc gggcggccgc tcg                      223 

 
           
             97  
             527  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(527)  
               n = A,T,C or G  
             
           
            97 

tcgagcggcc gcccgggcag gtctgtgcag gagacactga agtgggtagt gtccataatc     60 

tttttagcct gttgctgaaa ttccagttgt actccttcaa accaaaatgc ttacaggatc    120 

atgggaaagc ctcggttgca gaaatcaaga caggcaagtg ggaagataac tcggctttga    180 

ggttaaacag atctgggttc aaagcatagt ttcactctct gtcttgtgaa gtgtcctggg    240 

tgaagtcatt tcctctcttg aatttcagag aggatgaaaa tataaaaagt ataataacta    300 

tcttcataat ctttgtgagg attaaagaag acgaagtgtg tgaaaagcta agcacagagc    360 

aggcattcta caataagtag ttattatttt tggaaccatc ccgnccctag ccccagccca    420 

attaccttct cttagnctct tcatatcgaa ngccgtaatc ttgaccttct cttgcnactg    480 

gattggtgct ggttgatgcc caaacttccc gagatgctgt ctgggaa                  527 

 
           
             98  
             514  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(514)  
               n = A,T,C or G  
             
           
            98 

tcgagcggcc gcccgggcag gtctggctcc catggccctt ggggtggcct gactctgtca     60 

ctattcctaa aaccttctag gacatctgct ccaggaagaa ctttcaacac caaaattcat    120 

ctcaatttta cagatgggaa aagtgattct gagaccagac cagggtcagg ccaaggtcat    180 

ccagcatcag tggctgggct gagactgggc ccagggaacc ctgtctgctc ctctttttcc    240 

cagagctgtg agttctctag ccaaggctgc actcttgagg gagagccagg aagcatagct    300 

gaggccatga caacctcact cttcacctga aaatttaacc cgtggcagag gatccaggca    360 

catataggct tcggagccaa acaggacctc ggccgcgacc acgctaagcc gaattccagc    420 

acactggcgg ccgttactag tggatcccga gcttnggtac caagcttggc gtaatcatgg    480 

gcatagctgg ttcctggggt gaaaatggta tccg                                514 

 
           
             99  
             530  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(530)  
               n = A,T,C or G  
             
           
            99 

tcgagcggcc gcccgggcag gtctgaagaa acaggtataa atttggcagc cagtaatttt     60 

gacagggaag ttacagcttg catgacttta aatatgtaaa tttgaaaata ctgaatttcg    120 

agtaatcatt gtgctttgtg ttgatctgaa aaatataaca ctggctgtcg aagaagcatg    180 

ttcaaaaata tttaattcac ttcaaaatgt catacaaatt atggtggttt ctatgcaccc    240 

ctaaagcttc aagtcattta gctcaggtac atactaaagt aatatattaa ttcttccagt    300 

acagtggtgt ttcataccat tgacatttgc ataccctaga ataatttaag aaagacatgt    360 

gtaatattca caatgttcag aaaagcaagc aaaaggtcaa ggaacctgct ttggttcttc    420 

tggagatggn ctcatatcag cttcataaac attcattcta caaaatagta agctaaccat    480 

ttgaacccca atttccagat taagcatatt ttctcataaa tnatgaagcc               530 

 
           
             100  
             529  
             DNA  
             Homo sapien  
           
            100 

agcgtggtcg cggccgaggt ccaggcacgg tggcttatgt gtgtaatccc agcacttggg     60 

gaggctgagg gaggtggatc acttgagtcc aggagtttga gaccagtctg ggcaacatgg    120 

cgaaacttca tcactaccaa agaagaaaaa aattagccag gtgtggtggt gtatgcctgt    180 

agtcccagat actctggtgg ctgaggtgag aggatagctt gagcccagga aattgaggct    240 

gcagtgaact atgattgcac tactgtgctc cagcttgggc aacagagtga gatcttgtct    300 

ccaaaagtcc ttgaaggatt ttaggaagtt gttaaaagtc ttgaaacgat gtttgggggc    360 

atgttagggt tcttgaatgt ttaattcctc taataactgc ttattcaaga gaagcatttc    420 

tgactgggtg cggggcagtg gcttcatgcc ccataatccc agtactttgg gaggctgaag    480 

caggaacatt gcttgagccc aggacttcaa gaacagcctg ggtaacata                529 

 
           
             101  
             277  
             DNA  
             Homo sapien  
           
            101 

tcgagcggcc gcccgggcag gtcgcaggaa gaggatggaa actgaggagt ccaggaagaa     60 

gagggaacga gatcttgagc tggaaatggg agatgattat attttggatc ttcagaagta    120 

ctgggattta atgaatttgt ctgaaaaaca tgataagata ccagaaatct gggaaggcca    180 

taatatagct gattatattg atccagccat catgaagaaa ttggaagaat tagaaaaaga    240 

agaagagctg agaacagacc tcggccgcga ccacgct                             277 

 
           
             102  
             490  
             DNA  
             Homo sapien  
           
            102 

gcgtggtcgc ggccgaggtc tgacggcttt gctgtcccag agccgcctaa acgcaagaaa     60 

agtcgatggg acagttagag gggatgtgct aaagcgtgaa atcagttgtc cttaattttt    120 

agaaagattt tggtaactag gtgtctcagg gctgggttgg ggtccaaagt gtaaggaccc    180 

cctgccctta gtggagagct ggagcttgga gacattaccc cttcatcaga aggaattttc    240 

ggatgttttc ttgggaagct gttttggtcc ttggaagcag tgagagctgg gaagcttctt    300 

ttggctctag gtgagttgtc atgtgggtaa gttgaggtta tcttgggata aagggtcttc    360 

tagggcacaa aactcactct aggtttatat tgtatgtagc ttatattttt tactaaggtg    420 

tcaccttata agcatctata aattgacttc tttttcttag ttgtatgacc tgccccgggc    480 

ggccgctcga                                                           490 

 
           
             103  
             490  
             DNA  
             Homo sapien  
           
            103 

gagcggccgc ccgggcaggt ccaaaccagc ttgctcataa gtcattaacc aaatccatta     60 

taggtaattt gttcagttca atgtttacaa ttcttatgga aaaaattagc aacacacaca    120 

tttaaaacgt gtgcatttac ctttgcgtga gtgcttaaaa tacatatttc tatttcaaga    180 

tgacatttaa aaattattct aatatatcag cagcaaaaat ataatttgca attacaaaaa    240 

actaaactag aatccttaag ttattctcat gtttacagtt gtgattcttt aataaatact    300 

attatgcagc tctattgttt aagctttctg gatttggttt aaacacatgc atatatattg    360 

tcaattgtgg gaagctttac aagttatatt ccatgcactt tttggacaga gttctaacag    420 

agccagccag tccacaaaac aggcaagaca aaagttgaat taactggggc aaaataggac    480 

tcttatgcaa                                                           490 

 
           
             104  
             489  
             DNA  
             Homo sapien  
           
            104 

cgtggtcgcg gccgaggtcc aggctggtct cgaactcctg accttgtgat ctgcccgcct     60 

cggcctccca aagtgttggg attacaggca tgagccactg cgcccgaccg agttgaacat    120 

ttaatgtcag actaggccag agtttctcaa tctttttatt ctcacttccc aaaggagccg    180 

ttggagattt tcccctcaat ctctctcctt catgaaattt cataccacaa atatagtatg    240 

ttttatttat gtactgtgac cctttgaagg atcacaaacc aatataatag tttttctttt    300 

taacccgtca aggaccaagt ttttgcccct gttggaaatg cataaactgg actgatgaat    360 

tggtatagat ggcttttatc atgaggatca gaaaaacttg aaattccttg gctacgacac    420 

tccatattta tcaccgtata gggaggacct tggtatgggg aagtagaaac acttctacac    480 

tttacagca                                                            489 

 
           
             105  
             479  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(479)  
               n = A,T,C or G  
             
           
            105 

gcgtggtcgc ggccgaggtc tgactggctt cagccccaga agttgagctg gcctttagac     60 

aaaataattg cacctccctc tgctgcttat tcccttccgt ttttcatttg agtgtgaaca    120 

gttagataaa atctgtggct gnctcttcca ccttgctcta gtttccattg ctgtgagcag    180 

gccctcctat gccccgcatt tagctacaat gctgtggact cacttgattc tttttctccg    240 

agctttgtct agaaatatgt gaaggtgagg ttaagtgctt ctctgtgtag atccacttag    300 

ccctgtctgc tgtctcgatg ggcgttgctt cgtctctcct ctcttccatc ctttccattt    360 

gcttctcacc accttctggc ttcttttctt aatgcaataa aggcagtttc taacaaagaa    420 

agaatgtggg ctttggagtt agacagacct ggntttaaat tctgcttctg gctctccaa     479 

 
           
             106  
             511  
             DNA  
             Homo sapien  
           
            106 

tcgcggccga ggtccaaaac gtggattcca atgacctgcc ttgagcccgc ggttgccagg     60 

agttggacct gcagtagtat gggaagctca cggcctaaat accgactgcc ctctgacccc    120 

accgtccagc gattctagaa catttctagt aggaaagaca tagcaaggga ttttcatgat    180 

tgggaaatac tgggagacaa gctgaagatt tgttaagggc tatgcttctg tcatctttta    240 

ggtatttaag gctactcctt tagctagcta ctttgagctg tttaaagtga ctatctccct    300 

acacagagtt acacaatgag catctctgaa agagaatatt accctggatt tccaaagatg    360 

tactctaaca ggatgaccag gcaaaaggtg acccggggga ggagtctgtt ataacactcg    420 

gacccacatg ttctcaaggc acttcagaac tttgggaaat cattttgtac cggatcctca    480 

gaaagcattt atggaaatac acatccttta g                                   511 

 
           
             107  
             451  
             DNA  
             Homo sapien  
           
            107 

ggccgcccgg gcaggtccag aatatcaaat caaaaggtca caaatgttca cttcctcctc     60 

caccctctta catattggat cttcaattgc aatagggagt gtaagatggg cattttagag    120 

acgtagttgc atcagcagaa gcaaacccat cttatacaaa tgggttttgg ggataggaaa    180 

aggctgctaa aaattcacaa gtcaccattc cccagaagca atgaatagcc gtagaagacc    240 

aaggaagatc aacaagtttc caaagtgcta aagccagaga tttggccctt ccaaaatacc    300 

accaggacgc ctggacccgt gggctctccg catgtcacca ctgactgcca ggatgctgct    360 

gcacctccct tccttgagac acaacagaga gacagtgaag tcacccaaga ctgggatcat    420 

cagaggctcc tcatgcttgc tacagagaag c                                   451 

 
           
             108  
             461  
             DNA  
             Homo sapien  
           
            108 

ccgcccgggc aggtcctgaa aacattcaga ctaatcaaaa tggtactact gtaacttctt     60 

ataatacata atataaaagt ttttgaaaga tatagacaca attaacccct aaacaacaca    120 

ctatctgatt ctcaaaagca atggctattt aacaagatgt aaaaggacaa taacatatca    180 

aagaactttc acacacctaa agatagcatt tagcagcaag ttagtcagac aaaacaaaca    240 

caaatatttt cacatttcct atgtttgttt ttaactttac ttcataaagc cactgataat    300 

tgaggtttct ttcaagtata agatttctaa aattaaaaac tgtttttgac atatttttat    360 

aaagaaataa aaagcaaaac gcaatccaac tatttatatg agtccctctt ctccaacagc    420 

tttagatggt tttctgagta cttttttaca cagaatattt t                        461 

 
           
             109  
             441  
             DNA  
             Homo sapien  
           
            109 

ggccgcccgg gcaggtctga ttataagaga aagaaatcca gtgacacgag ggcaggcagg     60 

ccccgctctg ctctgatcga gaaaagcttc ctgatgtcag ggagatggaa ctgccaccat    120 

cagaaccatg gcactttggg tgaaggtgtg tcagcgacca agggggcagg aaatgggcag    180 

tgactaaggg ggcaggaaac aggcaggcac atggcaaggt tctcccagcc catcagccca    240 

gtgatggcct cgattttgaa gctgcactac tgtctgaaaa gcacaattac tggtgactct    300 

taacaaactt cagcatactg gggaaggaga ctgtcaagta actgaattgg aaagatgaaa    360 

aagaaccatc tctaaaagtt gatgcttgtc agaagaataa cctcctttgt gcaagtcttg    420 

caacatcttc attcaaccac a                                              441 

 
           
             110  
             451  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(451)  
               n = A,T,C or G  
             
           
            110 

ggtcgcggcc gaggtctggg gaaggggtga gaatccctgg gccttgccca gtcctgagct     60 

ctgggtgtct gcagggaagc acagtggtga gttagtgtta aagaaagcat ccagagaggt    120 

aagaggggct tgggtagcac cctttgcctc tgtcacttcc gcaaaaactt cttgttgagg    180 

aggaagatga gaaggttgac attgactttg gccttgttga agagtttcat gacagccaca    240 

ccctcatact ggagctgcan gagatcctga tagtgaagct tgaaatcgct ccatgtccac    300 

acccaggaac ttggcattta cttcaaactt tcctgcctca tctcccggcg tgatgtcaaa    360 

natgacgttt cttgaagtga gaggcgggaa agatcttcaa tttccaccaa agacaccctt    420 

tttccaggaa gcttgagcaa caagtgtaat g                                   451 

 
           
             111  
             407  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(407)  
               n = A,T,C or G  
             
           
            111 

ggccgacgtt cgacctgact tctttngagc agntgncact acccgtcttg aggaatgccg     60 

actgcagaca gtggcccang gcaaagagtg tgcgtcatcg atganattgg naagatggag    120 

ctcttcagtc agnttttcat tcaagctgnt cgtcagacgc tgtctacccc agggactata    180 

atcctnggca caatcccagt tcctanagga aagccactgn ctcttgtaga agaaatcana    240 

cacanaaagg atgtgaacng tgtttaatgt caccaaggga aaacatgaaa ccaccttctg    300 

ccagatatcg ggacgttgcg tgcagatcaa gcacgnaagt gaagacgcgt gcattccttg    360 

ccttccgtga acgantgccc agntcaagaa gancctgatg gaaccct                  407 

 
           
             112  
             401  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(401)  
               n = A,T,C or G  
             
           
            112 

tcgcggccga ggtcggccga ggtctgacat ctgttgtctg tgataaccac ttctgtattg     60 

cgtcttaacc acttctgtat tgtgtggttt taactgccta aggcggcaat gggcagtggg    120 

cccctttccc ttaggatggg tatcaattca acaatattta taaggcattt actgtgtgct    180 

aagcatttgg aagacccagg ctacaaaata agacatagtt cctgccctcc aggccagcag    240 

agggaggcac aaatacccag gaatctctga tgggtgtgaa gtgcggtcgt gggccacaga    300 

aaatgaccgt catggagacc ctgctaaagg tcggaccctg agcccaaagg ggtattcaga    360 

agnggagatg attttggccc cactcataga tgggtggcaa a                        401 

 
           
             113  
             451  
             DNA  
             Homo sapien  
           
            113 

gtcgcggccg aggtccatat taaaaagtcc atcataaaca aagactcctc ctcatggtat     60 

gaatatgctc catatgccca taatggtgca taacggactt agaaattcca atgagtctta    120 

gggttgaaat ttccaatgac ctgagcaagg cagctcccta tagcttctgg ataacatttt    180 

acacccagag ttcaggctta aacagaccta tcaacacaat tattttcgga ttgtctgtct    240 

agaaaacggc aatgctcaaa ggaatataaa taagggtggg gggacatatg cttccagcct    300 

ggcctttctc catgtggtaa aaaacaatgg aatggctgtg ttaatttttt tttaatcttt    360 

tctgaccttt actatgtttg gtaatggaaa taagtcaggg aaaacaaaat gaacaggtct    420 

catcacttaa ttaatactgg gttttcttct t                                   451 

 
           
             114  
             441  
             DNA  
             Homo sapien  
           
            114 

ggccgcccgg gcaggtccat cctgtcagag atgggagaag tcacagacgg aatgatggat     60 

acaaagatgg ttcactttct tacacactat gctgacaaga ttgaatctgt tcatttttca    120 

gaccagttct ctggtccaaa aattatgcaa gaggaaggtc agcctttaaa gctacctgac    180 

actaagagga cactgttgtt tacatttaat gtgcctggct caggtaacac ttacccaaag    240 

gatatggagg cactgctacc cctgatgaac atggtgattt attctattga taaagccaaa    300 

aagttccgac tcaacagaga aggcaaacaa aaagcagata agaaccgtgc ccgagtagaa    360 

gagaacttct tgaaacttga cacatgtgca aagacaggaa gcagcacagt ctcggcggga    420 

ggaagaaaaa aagaacagag a                                              441 

 
           
             115  
             431  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(431)  
               n = A,T,C or G  
             
           
            115 

gccgcccggg caggtccatt ggcggtgaca aaaggaaaag aagcaaagag actcagtcca     60 

taatgctgat tagttagaag aaagggctag gattgagaaa gtaccaggaa cttttaatta    120 

tttaaaagag aatgctgact gttaatgttt taaatcttac tgttcaaatg tactaatatg    180 

aatttttacc ctttgtgcat gaatattcta aacaactaga agacctccac aatttagcag    240 

ttatgaaagt taaacttttt attataaaaa ttctaaacct tactgctcct ttaccaggaa    300 

catgacacac tatttancat cagttgcata cctcgccaat agtataattc aactgtcttg    360 

cccgaacaat catctccatc tggaagacgt aagcctttag aaacacattt ttctattaat    420 

ttctctagaa c                                                         431 

 
           
             116  
             421  
             DNA  
             Homo sapien  
           
            116 

gtcgcggccg aggtccagaa atgaagaaga agtttgcaga tgtatttgca aagaagacga     60 

aggcagagtg gtgtcaaatc tttgacggca cagatgcctg tgtgactccg gttctgactt    120 

ttgaggaggt tgttcatcat gatcacaaca aggaaccggg gctcgtttat caccagtgag    180 

gagcaggacg tgagcccccg ccctgcacct ctgctgttaa acaccccagc catcccttct    240 

ttcaaaaggg atcctttcat aggagaacac actgaggaga tacttgaaga atttggattc    300 

agcccgcgaa gagatttatc aagcttaact cagataaaat cattgaaagt aataaggtaa    360 

aagctaagtc tctaacttcc aggcccacgg ctcaagtgaa tttcgaatac tgcatttaca    420 

g                                                                    421 

 
           
             117  
             489  
             DNA  
             Homo sapien  
           
            117 

agcgtggtcg cggccgaggt aaggctgcga ggttgtggtg tctgggaaac tccgaggaca     60 

gagggctaaa tccatgaagt ttgtggatgg cctgatgatc cacagcggag accctgttaa    120 

ctactacgtt gacactgctg tgcgccacgt gttgctcaga cagggtgtgc tgggcatcaa    180 

ggtgaagatc atgctgccct gggacccaac tggtaagatt ggccctaaga agcccctgcc    240 

tgaccacgtg agcattgtgg aacccaaaga tgagatactg cccaccaccc ccatctcaga    300 

acagaagggt gggaagccag agccgcctgc catgccccag ccagtcccca cagcataaca    360 

gggtctcctt ggcagacctg cccgggcggc cgctcgaaag cccgaattcc agcacactgg    420 

cggccgttac tagtggatcc cagctcggta ccaagcttgg cgtaatcatg gtcatagctg    480 

gtttcctgt                                                            489 

 
           
             118  
             489  
             DNA  
             Homo sapien  
           
            118 

tcgagcggcc gcccgggcag gtattgaata cagcaaaatt ctatatacaa agtgacctgg     60 

acctgctgct tcaaaacatg atcctttctt actaatatct tgatagtcgg tccatagagc    120 

attagaaagc aattgactct taaataaaca gaaaagtgcc taatgcacat taaatgaatg    180 

gcctaactac tggaacttta gtagttctat aaggtgatta acataggtag gatccagttc    240 

ctatgacagg ctgctgaaga acagatatga gcatcaagag gccattttgt gcactgccac    300 

cgtgatgcca tcgtgtttct ggatcataat gttcccatta tctgattcta gacacaccac    360 

aggaatatca gtggggtcag aggttagctt agctgcttgc tgggctagaa cagatatcac    420 

tccagcatgc tcatctgaca gggtcccgcg gcaacccaga ttaagtcctt gtgaatctgt    480 

gcacaggga                                                            489 

 
           
             119  
             181  
             DNA  
             Homo sapien  
           
            119 

taggttccag agacttttgg cccaggagga atatttactt ttagctctgg acatcattac     60 

aaaaaggaat atttcccaaa cctcttcaga ccgagaatac atgggtaaaa ttattaaata    120 

gttgtataat aaaaataatt ttttccttaa aaaaaaaaaa aacctcggcc gcgaccacgc    180 

t                                                                    181 

 
           
             120  
             489  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(489)  
               n = A,T,C or G  
             
           
            120 

gcgtggtcgc ggccgaggtc catttaaaac aaagaaaaat actaaagcca ctagtaaaca     60 

tctgatgtgc aaaatacaac atcctctagt tggctttatg ccattattac ataagctcca    120 

aatagctcat cttaaattaa aaagaaaaag tggctgtccc atctctgctg cataaatcag    180 

attttttttt aaaggtttag agtactttaa ggaagggaag ttcaaaactg ccagtgaaat    240 

tcacagagaa tacaaattta gcaatttaat ttcccaaagc tctttgaaga agcaagagag    300 

tctctcttct taatgcagtg ttctcccaag aggaactgta attttgcttg gtacttatgc    360 

tgggagatat gcaaaatgtg tttttcaatg tttgctagaa tataatggtt cctcttcagt    420 

gnctggttca tcctggaact catgggttaa gaaggacttc ttggagccga actgcccggg    480 

cgggccntt                                                            489 

 
           
             121  
             531  
             DNA  
             Homo sapien  
           
            121 

cgagcggccg cccgggcagg tggccagcgc tggtcccgca gacgccgaga tggaggaaat     60 

atttgatgat gcgtcacctg gaaagcaaaa ggaaatccaa gaaccagatc ctacctatga    120 

agaaaaaatg caaactgacc gggcaaatag attcgagtat ttattaaagc agacagaact    180 

ttttgcacat ttcattcaac ctgctgctca gaagactcca acttcacctt tgaagatgaa    240 

accagggcgc ccacgaataa aaaaagatga gaagcagaac ttactatccg ttggcgatta    300 

ccgacaccgt agaacagagc aagaggagga tgaagagcta ttaacagaaa gctccaaagc    360 

aaccaatgtt tgcactcgat ttgaagactc tccatcgtat gtaaaatggg gtaaactgag    420 

agattatcag gtcccgagga ttaaactggc tcatttcttt gtatgagaat ggcatcaatg    480 

gtatccttgc agatgaaatg ggcctaggaa agactcttca acaatttctc t             531 

 
           
             122  
             174  
             DNA  
             Homo sapien  
           
            122 

tcgagcggcc gcccgggcag gtctgccaac agcagaggcg gggcctccgg catcttcaaa     60 

gcacctctga gcaggctcca gccctctggc tgcgggaggg gtctggggtc tcctctgagc    120 

tcggcagcaa agcagatgtt atttctctcc cgcgacctcg gccgcgacca cgct          174 

 
           
             123  
             531  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(531)  
               n = A,T,C or G  
             
           
            123 

agcgtggtcg cggccgaggt cctcaaccaa gagggttgat ggcctccagt caagaaactg     60 

tggctcatgc cagcagagct ctctcctcgt ccagcaggcg ccatgcaagg gcaggctaaa    120 

agacctccag tgcatcaaca tccatctagc anagagaaaa ggggcactga agcagctatg    180 

tctgccaggg gctaggggct cccttgcaga cagcaatgct acaataaagg acacagaaat    240 

gggggaggtg ggggaagccc tatttttata acaaagtcaa acagatctgt gccgttcatt    300 

cccccagaca cacaagtaga aaaaaaccaa tgcttgtggt ttctgccaag atggaatatt    360 

cctccttcct aanttccaca catggccgtt tgcaatgctc gacagcattg cactgggctg    420 

cttgtctctg tggtctgggc accagtagct tgggccccat atacacttct cagttcccac    480 

anggcttatg gccnangggc angctccaat tttcaagcac cacgaaggaa g             531 

 
           
             124  
             416  
             DNA  
             Homo sapien  
           
            124 

tcgagcggcc gcccgggcag gtccatctat actttctaga gcagtaaatc tcataaattc     60 

acttaccaag cccaggaata atgactttta aagccttgaa tatcaactaa gacaaattat    120 

gccaattctg atttctcaca tatacttaga ttacacaaag ataaagcttt agatgtgatc    180 

attgtttaat gtagacttat ctttaaagtt tttaattaaa aactacagaa gggagtaaac    240 

agcaagccaa atgatttaac caaatgattt aagagtaaaa ctcactcaga aagcattata    300 

cgtaactaaa tatacatgag catgattata tacatacatg aaactgcaat tttatggcat    360 

tctaagtaac tcatttaagt acatttttgg catttaaaca aagatcaaat caagct        416 

 
           
             125  
             199  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(199)  
               n = A,T,C or G  
             
           
            125 

agcgtggtcg cggccgaggt gctttttttt tttttttttt tttttttttt gctattctaa     60 

aggggaaggc ccctttttat taaacttgta cattttactt tccttctttc anaatgctaa    120 

taaaaaactt ttgtttatac ttaaaaaaac cataaatcan acaaacaaaa gaaacgattc    180 

caacatcact tctgngatg                                                 199 

 
           
             126  
             490  
             DNA  
             Homo sapien  
           
            126 

cgtggtcgcg gccgaggtcc agttgctcta agtggattgg atatggttgg agtggcacag     60 

actggatctg ggaaaacatt gtcttatttg cttcctgcca ttgtccacat caatcatcag    120 

ccattcctag agagaggcga tgggcctatt tgtttggtgc tggcaccaac tcgggaactg    180 

gcccaacagg tgcagcaagt agctgctgaa tattgtagag catgtcgctt gaagtctact    240 

tgtatctacg gtggtgctcc taagggacca caaatacgtg atttggagag aggtgtggaa    300 

atctgtattg caacacctgg aagactgatt gactttttag agtgtggaaa aaccaatctg    360 

agaagaacaa cctaccttgt ccttgatgaa gcagatagaa tgcttgatat gggctttgaa    420 

ccccaaataa ggaagattgt ggatcaaata agacctgata ggcaaactct aatgtggagt    480 

gcgacttggc                                                           490 

 
           
             127  
             490  
             DNA  
             Homo sapien  
           
            127 

cgtggtcgcg gccgaggtcg gccgaggtct ggagatctga gaacgggcag actgcctcct     60 

caagtgggtc cctgacccct gacccccgag cagcctaact gggaggcacc ccccagcagg    120 

ggcacactga cacctcacac ggcagggtat tccaacagac ctgaagctga gggtcctgtc    180 

tgttagaagg aaaactaaca agcagaaagg acagccacat caaaaaccca tctgtacatc    240 

accatcatca aagaccaaaa gtaaataaaa ccacaaagat gggaaaaaaa cagaacagaa    300 

aaactggaaa ctctaaaaag cagagcacct ctcctcttcc aaaggaacgc agttcctcac    360 

cagcaatgga acaaagctgg atggagaatg actttgacga gctgagaaaa gaacgcttca    420 

gacgatcaaa ttactctgag ctacgggagg acattcaaac caaaggcaaa gaagttgaaa    480 

actttgaaaa                                                           490 

 
           
             128  
             469  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(469)  
               n = A,T,C or G  
             
           
            128 

cgtggtcgcg gccgaggtgc tttttttttt tttttttttt tttttttttt tgctgattta     60 

ttttttctnt ttattgttac atacaatgta taaacacata aaacanaaaa cagtagggat    120 

cctctaggat ctctagggan acagtaaagt anaaagaggt ctcanaaaca tttttttaaa    180 

gtacaagaca ttcagngctc ggcccaaagg cgtaaaaggt ttanagccag canatagctg    240 

nactaaaggc tccgtctntn tccccanagc caggacaacc ccagggagct ntccattagc    300 

agccagtcca cgcaggcagg atgctgcgga aaaagctcta tgctganaac attccccttg    360 

atggaaagaa gggcaacaca aaaggggtaa ctaanagctc cttcctctcg tgagggcgac    420 

aactgaggaa cagaaaagga gtgtcccatg tcacttttga ccccctccc                469 

 
           
             129  
             419  
             DNA  
             Homo sapien  
           
            129 

gcgtggtcgc ggccgaggtc tgattttcat ttaaatattt cagagctata gcatttgcct     60 

ccatgctcaa atccacacca ttggggctta agccgctcat gccaacatta gcaaatgaca    120 

tgcagtttaa tccagagatc actgcttctg ggctgatgca tgccaacaca ctggcgtgat    180 

ccacgttatg tgcatttttc ttcactttag tgggagaatc aatttttact ccaaggcttc    240 

ttagttgctt aagagttgca ttaaggacac aatctttgtc caccagtctt gaatgatgtg    300 

tttttttctt tgtatggtaa acgttttggg ttctggtgca ttcatgactg ataattactg    360 

ctttggtaga cggctgctca agtttccttg gaggaactat ttaataggtg ggttacttg     419 

 
           
             130  
             354  
             DNA  
             Homo sapien  
           
            130 

agcgtggtcg cggccgaggt ccatctgagg agataaccac atcactaaca aagtgggagt     60 

gaccccgcag agcacgctgt ggaattccat agttggtctc atccctggtc agtttccaca    120 

tgatgatggt cttatctcga gaggcggaga ggatcatgtc cgggaactgc ggggtagtag    180 

cgatctgggt tacccagccg ttgtggccct tgagggtgcc acgaagggtc atctgctcag    240 

tcatggcggc ggcgagagcg tgtgtcgctg cagcgacgag gatggcactg gatggcttag    300 

agaaactagc accacaacct ctcctgccgc acctgcccgg gcggcccgct cgaa          354 

 
           
             131  
             474  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(474)  
               n = A,T,C or G  
             
           
            131 

cgagcggccg cccgggcagg tctggcagca gcttcctctg gaataattga cagctttgtg     60 

ctgcctgact aaaatttgaa atgacaaccg ctgaatgtaa aatgatgtac ctacaatgag    120 

agagatttag gaatactatc tgtcaatcca tagatgtaga aacaaaacaa actacagaat    180 

gaaaacaaac ttattttaaa ccaaagaaac aaatgtatcc aaaatatagt ccatgatata    240 

tttgattact agtataacca cagttgaaaa cttaaaaaaa aaaattgaca ttttttgtaa    300 

tgggtactaa tggatttata aaaggtttct gtttccaaag atgttattgg ggtccacata    360 

ttccttgaag acttcagcat cccaaagccc gacatcagag atactttcct ttagccattg    420 

nttcccgtaa cttgcccact ccatggtgat gtgacaggct tcccttcatt agca          474 

 
           
             132  
             474  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(474)  
               n = A,T,C or G  
             
           
            132 

ggccgaggtg gggaattcat gtggaggtca gagtggaagc aggtgtgaga gggtccagca     60 

gaaggaaaca tggctgccaa agtgtttgag tccattggca agtttggcct ggccttagct    120 

gttgcaggag gcgtggtgaa ctctgcctta tataatgtgg atgctgggca cagagctgtc    180 

atctttgacc gattccgtgg agtgcaggac attgtggtag gggaagggac tcattttctc    240 

atcccgtggg tacagaaacc aattatcttt gactgccgtt ctcgaccacg taatgtgcca    300 

gtcatcactg gtagcaaaga tttacagaat gtcaacatca cactgcgcat cctcttccgg    360 

cctgtcgcca gccagcttcc tcgcatcttc accagcatcg ganaggacta tgatgaaccg    420 

tgtgctgccg tccatcacaa ctgagatcct caagtcagtg gtggctcgct ttga          474 

 
           
             133  
             387  
             DNA  
             Homo sapien  
           
            133 

tgctcgagcg gccgccagtg tgatggatat ctgcagaatt cggcttagcg tggtcgcggc     60 

cgaggtctgc gggcccctta gcctgccctg cttccaagcg acggccatcc cagtagggga    120 

ctttcccaca ctgtgccttt acgatcagcg tgacagagta gaagctggag tgcctcacca    180 

cacggcccgg aaacagcggg aagtaactgg aaagagcttt aggacagctt agatgccgag    240 

tgggcgaatg ccagaccaat gatacccaga gctacctgcc gccaacttgt tgagatgtgt    300 

gtttgactgt gagagagtgt gtgtttgtgt gtgtgttttg ccatgaactg tggccccagt    360 

gtatagtgtt tcagtggggg agaactg                                        387 

 
           
             134  
             401  
             DNA  
             Homo sapien  
           
            134 

ggccgcccgg gcaggtctga tgaagaacac gggtgtgatc cttgccaatg acgccaatgc     60 

tgagcggctc aagagtgttg tgggcaactt gcatcggctg ggagtcacca acaccattat    120 

cagccactat gatgggcgcc agttccccaa ggtggtgggg ggctttgacc gagtactgct    180 

ggatgctccc tgcagtggca ctggggtcat ctccaaggat ccagccgtga agactaacaa    240 

ggatgagaag gacatcctgc gcttgtgctc acctccagaa ggaagttgct cctgagtgct    300 

attgactctt gtcaatgcga ccttcaagac aggaggctac ctggtttact gcacctgttc    360 

tatcacagtg agacctctgc catggcagaa caggggaagc t                        401 

 
           
             135  
             451  
             DNA  
             Homo sapien  
           
            135 

ggtcgcggcc gaggtctgtt cctgagaaca gcctgcattg gaatctacag agaggacaac     60 

taatgtgagt gaggaagtga ctgtatgtgg actgtggaga aagtaagtca cgtgggccct    120 

tgaggacctg gactgggtta ggaacagttg tactttcaga ggtgaggtgt cgagaaggga    180 

aagtgaatgt ggtctggagt gtgtccttgg ccttggctcc acagggtgtg ctttcctctg    240 

gggccgtcag ggagctcatc ccttgtgttc tgccagggtg gggtaccggg gtttgacact    300 

gaggagggta acctgctggc tggagcggca gaacagtggc cttgatttgt cttttggaag    360 

attttaaaaa ccaaaaagca taaacattct ggtccttcac aatgctttct ctgaagaaat    420 

acttaacgga aggacttctc cattcaccat t                                   451 

 
           
             136  
             411  
             DNA  
             Homo sapien  
           
            136 

ggccgcccgg gcaggtctga atcacgtaga atttgaagat caagatgatg aagccagagt     60 

tcagtatgag ggttttcgac ctgggatgta tgtccgcgtt gagattgaaa atgttccctg    120 

tgaatttgtg cagaactttg acccccttta ccccattatc ctgggtggct tgggcaacag    180 

tgagggaaat gttggacatg tgcaggtggg tccctttgct gcgtatttgg tgcctgaggc    240 

tctgtggatt tcccctccat caatcatctt accctctcat ccccctcaga tgcgtctgaa    300 

gaaacatctc tggtataaga aaatcctcaa gtcccaagat ccaatcatat tttctgtagg    360 

gtggaggaag tttcagacca tcctgctcta ttatatccga agaccacaat g             411 

 
           
             137  
             211  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(211)  
               n = A,T,C or G  
             
           
            137 

cggccgcccg ggcaggtcgg ttggtgcggc ctccattgtt cgtgttttaa ggcgccatga     60 

ggggtgacag aggccgtggt cgtggtgggc gctttggttc cagaggaggc ccaggaggag    120 

ggttcaggcc ctttgcacca catatcccat ttgacttcta tttgtgtgaa atggcctttc    180 

cccggntcaa gccagcacct cgatgaaact t                                   211 

 
           
             138  
             471  
             DNA  
             Homo sapien  
           
            138 

gccgcccggg caggtctggg ctggcgactg gcatccaggc cgtaactgca aatctatgct     60 

aggcggggtc tcccttctgt gtgttcaagt gttctcgact tggattctta actattttaa    120 

aaaatgcact gagtttgggt taaaaaccaa ccaccaaaat ggatttcaac acagctctaa    180 

agccaagggc gtggccggct ctcccaacac agcgactcct ggaggccagg tgcccatggg    240 

cctacatccc ctctcagcac tgaacagtga gttgattttt ctttttacaa taaaaaaagc    300 

tgagtaatat tgcataggag taccaagaaa ctgcctcatt ggaaacaaaa actatttaca    360 

ttaaataaaa agcctggccg caggctgcgt ctgccacatt tacagcacgg tgcgatgcac    420 

acggtgacca aaccacggag gcaagcttct ggcactcaca ccacgacccg c             471 

 
           
             139  
             481  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(481)  
               n = A,T,C or G  
             
           
            139 

gtcgcggccg aggtctgttc tttagctcag atttaaacct gctgtctctt ctttatttgc     60 

agaatgaatt cccagttcct gagcagttca agaccctatg gaacgggcag aagttggtca    120 

ccacagtgac agaaattgct ggataagcga agtgccactg ggttctttgc cctcccttca    180 

caccatggga taaatctgta tcaagacggt tcttttctag atttcctcta cctttttgct    240 

cttaaaactg cttctctgct ctgagaagca cagctacctg ccttcactga aatatacctc    300 

aggctgaaat ttggggtggg atagcaggtc agttgatctt ctgcaggaag gtgcagcttt    360 

tccatatcag ctcaaccacg ccgncagtcc attcttaagg aactgccgac taggactgat    420 

gatgcatttt agcttttgag cttttggggg gtattctacc aaccaacagt ccatttggaa    480 

a                                                                    481 

 
           
             140  
             421  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(421)  
               n = A,T,C or G  
             
           
            140 

gtcgcggccg aggtttccca tttaagaaaa atagatcttg agattctgat tcttttccaa     60 

acagtcccct gctttcatgt acagcttttt ctttacctta cccaaaattc tggccttgaa    120 

gcagttttcc tctatggctt tgcctttctg attttctcag aggctcgagt ctttaatata    180 

accccaaatg aaagaaccaa ggggaggggt gggatggcac ttttttttgt tggtcttgtt    240 

ttgttttgtt ttttggttgg ttgggttccg ttatttttta agattagcca ttctctgctg    300 

ctatttccct acataatgtc aatttttaac cataattttg acatgattga gatgtacttg    360 

aggctttttt gntttaattg agaaaagact ttgcaatttt ttttttagga tgagcctctc    420 

c                                                                    421 

 
           
             141  
             242  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(242)  
               n = A,T,C or G  
             
           
            141 

cgantngccc gcccgggcan gtctgtctaa ntttntcang gaccacgaac agaaactcgt     60 

gcttcaccga anaacaatat cttaaacatc gaanaattta aatattatga aaaaaaacat    120 

tgcaaaatat aaaataaata nnaaaaggaa aggaaacttt gaaccttatg taccgagcaa    180 

atccaggtct agcaaacagt gctagtccta nattacttga tntacaacaa cacatgaata    240 

ca                                                                   242 

 
           
             142  
             551  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(551)  
               n = A,T,C or G  
             
           
            142 

agcgtggtcg cggcncgang tccacagggc anatattctt ttagtgtctg gaattaaaat     60 

gtttgaggtt tangtttgcc attgtctttc caaaaggcca aataattcan atgtaaccac    120 

accaagtgca aacctgtgct ttctatttca cgtactgttg tccatacagt tctaaataca    180 

tgtgcagggg attgtagcta atgcattaca cagtcgttca gtcttctctg cagacacact    240 

aagtgatcat accaacgtgt tatacactca actagaanat aataagcttt aatctgaggg    300 

caagtacagt cctgacaaaa gggcaagttt gcataataga tcttcgatca attctctctc    360 

caaggggccc gcaactaggc tattattcat aaaacacaac tgaanagggg attggtttta    420 

ctggtaaatc atgtgntgct aaatcatttt ctgaacagtg gggtctaaat cantcattga    480 

tttagtggca gccacctgcc cggcggccgn tcgaagccca attctgcaga tatccatcac    540 

actggcggcc g                                                         551 

 
           
             143  
             515  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(515)  
               n = A,T,C or G  
             
           
            143 

cgagnggccc gcccgggcag gtatcttcac aaactcaaca aaggcactac atgagacttc     60 

acattcccct agtccaatag ctgacaaatt tttgcaacgt tctgcaatgc gaattaactc    120 

ttcatcaagt ggccgtaatc catttgcaca cactactagt tcaaccagtc tagggcatgt    180 

cattcccaca cggccaagca catctttgct tactgatctc ccaaagtaca gatgggtggc    240 

aggtatttca tagcgaaaga aggggtcaaa ttcttcttca tataanaaaa aatacatcac    300 

taagttcact ttgggtgaat gtctgatgaa agcatcccag ctactcttct gaatagtatg    360 

gaagtgtgtc tgtccaggat tctcactgac tacatcaatg cgcaaatgtt ctaatcgaac    420 

atgtttttca gaagacaatg caagtaacaa ctcatcactc aataagtggt aagttcaggg    480 

ctagttctct taagccgnga cactgatcag cacac                               515 

 
           
             144  
             247  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(247)  
               n = A,T,C or G  
             
           
            144 

tgcattctct ntggatgcan acctgcccgt tggtagggac tntgctcaca cggaacatgg     60 

acggttacac ctgtgccgtg ggtgacgtcc accagcttct ggatcatctc ggcgngggtg    120 

ttgtggaagg gcagactatc cacctccatg cncacgatgc ccganacgcc actccggact    180 

ntgtgctgca ccaanatgcc cagcattnta tcttcaagca nagcacttat cagggtcctt    240 

ggcacac                                                              247 

 
           
             145  
             309  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(309)  
               n = A,T,C or G  
             
           
            145 

cgtgggtcgc ggcccgangt ctgctgtaac aaaacaccat agtctgggca gctcatagac     60 

aatggaattt tatttctcac gcttctggag gctggattcc aagatcaagg ttccaggaga    120 

ctcagtgtct ggcaaggtct cggtttctgc ctcanagatg gtgccatctg gctgtgtcct    180 

cacaagtagg aaggtgcaag aagctcccct caggctctgt ctgtaagaca ctgatcccat    240 

tcatganggg gaaacgtaat gacctaatca gcccccagag accccacttc taacaccatc    300 

accttgggg                                                            309 

 
           
             146  
             486  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(486)  
               n = A,T,C or G  
             
           
            146 

agcgtgggtc gcggcncgac gtcctgtcca tatttcacag cccgagaact aatacaagat     60 

gctgacatca tattttgtcc ctacaactat cttctanatg cacaaataag ggaaagtatg    120 

gatttaaatc tgaaagaaca ggttgtcatt ttanatgaag ctcataacat cgaggactgt    180 

gctcgggaat cagcaagtta cagtgtaaca gaagttcagc ttcggtttgc tcgggatgaa    240 

ctanatagta tggtcaacaa taatataagg aaganagatc atgaacccct acgagctgtg    300 

tgctgtagcc tcattaattg gntagaagca aacgctgaat atcttgnana angagantat    360 

gaatcagctt gtaaaatatg gagtggaaat gaaatgctct taactttaca caaaatgggt    420 

atcaccactg ctacttttcc cattttgcng gtaagatatn ttttctacct gngaaacgta    480 

tttaag                                                               486 

 
           
             147  
             430  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(430)  
               n = A,T,C or G  
             
           
            147 

gccgcccggg cangttcgac attacntnga gttccatgat gtacaattct ttcacgaaaa     60 

acaatgaatg caagaatttg aggatctcct tactcctccc ttttacagat ggtctctcaa    120 

tcccttcttc ttcctcttca tcttcatctt cttctgaacg cgctgccggg taccacggct    180 

ttctttgtct ttatcgtgag atgaaggtga tgcttctgtt tcttctacca taactgaaga    240 

aatttcgctg caagtctctt gactggctgt ttctccgact tcgcctttnt gtcaaacgng    300 

agtcttttta cctcatgccc ctcagcttca cagcatcttc atctggatgt tnatttctca    360 

aagggctcac tgaggaaact tctgattcan atgtcgaana gcactgtgaa gttttctctt    420 

cattttgctg                                                           430 

 
           
             148  
             483  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(483)  
               n = A,T,C or G  
             
           
            148 

cccgggcagg tctgtgttgn tttncaaccg gtgtcctccc cagcgtccag aananggaaa     60 

tgtggagcgg gtgatgatga cccctcgctg tcctgtcacc tcctgcacag cttcgtatgt    120 

gggtctggtc tgggaccacc cgtacaggtt gtgcacgttg tagtgctcca cgggggagct    180 

gtccggcagg atctgctgac tctccatgca cagagtcttg ctgctcaggc ccttgtccct    240 

agattccaaa tatggcatat agggtggggt tatttagcat ttcattgctg cagcccctga    300 

cagatccatc cacaaaattt gatggctcat tcatatcaat ccacaatcca tcaaacttca    360 

agctcttctc tggntctcga nggtttgcat agaactcttc tatctctttc ttccaccacg    420 

canacctcgg ncgcgaccac gctaagccga attctgcana tatccatcac actggcggcc    480 

gct                                                                  483 

 
           
             149  
             439  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(439)  
               n = A,T,C or G  
             
           
            149 

ctttcacgaa nacaatgaat gcaagaattt gaggatctcc ttactcctcc cttttacaga     60 

tggtctctca atcccttctt cttcctcttc atcttcatct tcttctgaac gcgctgccgg    120 

gtaccacggc tttctttgtc tttatcgtga gatgaaggtg atgcttctgt ttcttctacc    180 

ataactgaag aaatttcgct gcaagtctct tgactggctg tttctccgac ttcgcctttt    240 

tgcaaacgtg agtcttttta cctcatgccc ctcagcttcc acagcatctt catctggatg    300 

ttcatttctc aaagggctca ctgaggaaac ttctgactca catgtcgaag aagcactgng    360 

agtttctctt catttgctgc aaanttgctc tttgctggct gngctctcag accacccatt    420 

tggctgcatg ggggctgac                                                 439 

 
           
             150  
             578  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(578)  
               n = A,T,C or G  
             
           
            150 

ggcncgcccg ggcangtcca ctccactttt gagctctgag ggaatacctt caggagggac     60 

agggtcaggg agtcctggca gctccgcagc agagattcac attcattcag agacttgttg    120 

tccagtgcaa tgccattgat cgcaacgatc ctgtctccca cagcaaggga cccttcttta    180 

gcggcagggc ttccaggcag cacagcggca gcatacactc cattctccag actgatgcca    240 

ctgtctttct gtccactgan gttgatgtgc agcggcgtga ccaccttccc acccagggac    300 

ttcctccgcc gcacgaccat gttgatgggc cccctnccca ttgaggagcg ccttgatggc    360 

ctgcttcttg nccttggtga tgaagtccac atcggtgatt ctcacagcca gtcattgacc    420 

cttaagcggn catcagcaat gcttcctttg gccactttag ngacaaatat gccacagtcc    480 

ccgggaaaca agggtcattc acaccttctg gcatatcaaa cacctcggcc gggancacta    540 

agccgaattc tgcagatatc catcacactg gngggccg                            578 

 
           
             151  
             503  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(503)  
               n = A,T,C or G  
             
           
            151 

cgagcggccc gcccgggcag gtctgggaga tcagcgactg ctgccacgtg cccagaaatg     60 

gctcgtcctt tcactacagc ggaatgcaat gagggtgggt gagaagatga tgggtcggtt    120 

atttcattcc ttttcttttt acaacttcac tttcagagac ttcagcgttc catgtctgct    180 

gtgctgtgga acccagagtg ctcttgcctg gatggctgag aatcccttgg accctggaag    240 

cacctactcc atgatggccc ggtatagtgc aggctcaata taatcttccc ggtatcttga    300 

gttgataact cgttgccgtt tcttttcttg cttaacctct ttctctgtga aaatctcatt    360 

gaagcgcatg tctgaagcta ctgacagtct anatttgact ctcttgggaa gctcttcatc    420 

cagtgtgtat acatcatctc tcttaaccac aagttggagc catncttaaa cttcacctgg    480 

tacatttgga tagggtggga ggc                                            503 

 
           
             152  
             553  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(553)  
               n = A,T,C or G  
             
           
            152 

agcgtggtcg cggcccgagg tccactgagc tccgccttcc ccgggctccc tgaggaagca     60 

gagtcctgac ttccaggaag gacaggacac agaggcaaga actcagcctg tgaggctctg    120 

ggtggctcct gaggccagag gacgccttcc gcgatccatg gctcagcatc gtccttctgg    180 

cttcccagcc ccgggccgaa cgttcgggtt aataagcaga gcagttattc ggctcctggc    240 

aggagctccc ccgttagttt ccacgttgtg agcacattca tacttaagac tgnttctctt    300 

tgtgttttaa gcgtctgtct ctgtagtaaa ctgaaatgtt aacagaaatg cagacctgcc    360 

cgggcggccg ctcgaaagcc gaattctgca gatatccatc acactggcgg ccgctcgagc    420 

atgcatctag anggcccaat tcgccctata gtgagtcgna ttacaattca ctgggccgcg    480 

ntttacaacg tcgtgactgg gaaaaccctg cggtacccac ttaatcgcct tgcagnacat    540 

ccccctttcg cca                                                       553 

 
           
             153  
             454  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(454)  
               n = A,T,C or G  
             
           
            153 

tcgagcggct cgcccgggca ggtccaccta gcatggctcc tctaaacacg caactcagcg     60 

aggggacccc cttcacctct ggcaagagag ctgggtagat cagaaacttg gtgacacctg    120 

gctagcacag agcaggctca cttgtcttgg tcccactacc cagattcctg cagacattgc    180 

aaaccaaatg aaggttgntg aatgacccct gtccccagcc acttgttttg gtatcatctg    240 

ctctgcagtg gaatgcctgt gtgtttgagt tcactctgca tctgtatatt tgagtataga    300 

aaccgantca agtgatctgt gcatncagac acactggggc acctgancac agaacaaatc    360 

accttaacga tctggaatga aactgnganc antgcccgcc tgggtgggtc tgganaaact    420 

gccgncttct tgttggacct tggccgcacc acct                                454 

 
           
             154  
             596  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(596)  
               n = A,T,C or G  
             
           
            154 

agcgtggtcg cggcccgang gcggcctcct gantganggg aagggacgtg ggggcggcca     60 

cggcaggatt aacctccatt tcagctaatc atgggagaga ttaaagtctc tcctgattat    120 

aactggttta naggtacagt tccccttaaa aagattattg tggatgatga tgacagtaag    180 

atatggtcgc tctatgacgc gggcccccga agtatcaggt gtcctctcat attcctgccc    240 

cctgtcagtg gaactgcaga tgtctttttc cggcagattt tggctctgac tggatggggt    300 

taccgggtta tcgctttgca gtatccagtt tattgggacc atctcgagtt cttgtgatgg    360 

attcacaaaa cttttanacc atttacaatt ggataaagtt catctttttg gcgcttcttt    420 

gggangcttt ttggcccana aatttgctga atacactcac aaatctccta gaagccattc    480 

cctaatcctc tgcaattcct tcagngacac ctctatcttc aaccaacttg gactggaaac    540 

agctttggct gatgcctgca tttatgctca aaaaatagtt cttggaaatt ttcatc        596 

 
           
             155  
             343  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(343)  
               n = A,T,C or G  
             
           
            155 

ctcganttgg cncgcccggg cangtctgcc tggtttttga ccgngcgagc tatttagnct     60 

ctggctctgt ttccggagct caaggnaaaa atcttgaana actcgagcag cttctgtgga    120 

tagccttggg tacacatact gccgagcata gccaatgtac tttctcaata gctggtgggg    180 

aatgggatct attgtttctc caggaaccac ctttagtctt tctgataatg gcttctcaga    240 

aactacttca agtacggaag tatttgaatc ttgactatnc atacgagcta ctgtggcact    300 

gctaatgggn tctctgctnt ccagctctta ttgcaatcac atg                      343 

 
           
             156  
             556  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(556)  
               n = A,T,C or G  
             
           
            156 

tcgagcggcc cgcccgggca ggtctggcac cacncagatc gattaactgg ctcatctgat     60 

ctcgtggccc ccaccctgga actgacttag cacaaaagga cacctcaatt ccttatgatt    120 

tcatctccga cccaaccaat caacaccctt gactcactgg ccttccccct cccaccaaat    180 

tatccttaaa aactctgatc cccgaatgct cagggagatc gatttgagta ctaataagac    240 

tccagtctcc tgcacaagca gctctgtgta ctcttcctct attgcaattc ctgtcttgat    300 

aaatcggctc tgtgtaggcg gcggaagaag tgaacctgtt gggcggttac cacctctgtc    360 

gtgtgtgaca gttgntttga atctctaatt gctcagtaca gatccacatg caggttaagt    420 

aagaagcttt tgaagaaaat ggaaagtctt aagtgatggc ttccaagaaa tcaaacctac    480 

attaattagg gaacaacgga ctttacgtat cacaaatgaa gagactgacn aagtaaatca    540 

acttggcctt ttctta                                                    556 

 
           
             157  
             333  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(333)  
               n = A,T,C or G  
             
           
            157 

ggtccacaaa aatatatnaa ataagctgga tatataaaan caaacactta acatngncan     60 

cattccttca gttattcaaa ctcactgata nctaacnggg agnagttggn attctggaag    120 

acttcctaag ctaaaagtat atttacatat ttacaacaca ngtaaatata acngaagaac    180 

tacttcaaat aangnngaaa ttccagaatt ctanagattt atagctatag ntnacaanta    240 

tcaccaattg gtttgcaatc aanngnccag cactacttat gannaangtt taactannaa    300 

accaaaaggg gagaaaacct ggnagggaaa nat                                 333 

 
           
             158  
             629  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(629)  
               n = A,T,C or G  
             
           
            158 

tcgagcggcc gcccgggcag gtctggtaca tttgtgcgag gtccggcact ctgttctcat     60 

ccagtaagtg gtcgagccct ttctgcagaa ttgctgttaa atgttctcct aatagctgtt    120 

tctccacaca agcaatcagt ggtttctgtg tgctgtggtc caagtaagtg attactctgt    180 

ctccctcttc ttctaagcgt ttacttacat ggttaagata ttctggaacc tctctttcct    240 

gcattaacct ttggccttcg gcagcatata agcaattagt ctcttccaaa aatttcagtt    300 

caaatgaatc tttatacacc tgcaggtcag acagcatgcc caggnaggct ccgcaacagg    360 

ctccggtcca cggcctcgcc gctcctctcg cgctcgatca gcagtaggat tccatcaatg    420 

gttttactct gaaccatttt atcactaata atatgggttc taaacagttc taatcccata    480 

tcccagatgg agggcagcgt ggagttctgc agcacatagg tgcggtccaa gaacaggaag    540 

atgcttctga tcatgaatca tttgnctggc aatggtcctg ccagcacgtg gtaatctttc    600 

ttttaaaaat aaacccttat ctaaacgtc                                      629 

 
           
             159  
             629  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(629)  
               n = A,T,C or G  
             
           
            159 

tcgagcggcc gcccgggcag gttctagagg ganaatctgg ctgatttggg aataaaatat     60 

aatcgaatat tcaacaccat gaagataaat cttattttgg aaatctactg accttaatac    120 

cccaagcttg ccctgaatac tttgattgga attggaatat atcaaaaaag gttagtattt    180 

ttgttgtagt taggatacta aaaggatatt agttacccaa gagatccaat ttgtttttct    240 

gatgaatagt gttcagtaaa atgaagcagt cttaagagtg actaataatt tcaaagtgat    300 

ttttcgtcta ttcttaatat tttttaatta tttattttta agagttttat accttgagca    360 

gatacaatga tccgctttag tgagaggaca atttctgatt gattgttttc tcttcaggcc    420 

atctcacctc ttcattctct tgttacattt gaagcagttg atataatggg tttatacttt    480 

aaaagataga catggtgcca tgaagtttgg ggaagttggg tgaattatcc cattctagtt    540 

acagangagc tttccttaaa tgccctttac ttctangttt ggtcaagaag tcattttctg    600 

agtaaaagtt attttcatat atgttgggg                                      629 

 
           
             160  
             519  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(519)  
               n = A,T,C or G  
             
           
            160 

tcgagcggcg cgcccgggca ggtctgctgg gattaatgcc aagttnttca gccataaggt     60 

agcgaaatct agcagaatcc agattacatc cacttccaat cacgcggtgt ttgggtaatc    120 

cacttagttt ccagataaca tacgtaagaa tgtccactgg gttggaaacc acaattatga    180 

tgcaatcagg actgtacttg acgatctgag gaataatgaa tttgaagaca ttaacatttc    240 

tctgcaccag attgagccga ctctcccctt cttgctgacg gactcctgca gttaccacta    300 

caatcttana attgggcggg tcacagaata atctttatct gccacaattt taggtgctga    360 

agaaataagc tcccatgctg cagatccatc atttctnctt taagcttatc ttccaaaaca    420 

tccacaagan caangttcat cagccagaga ctttcccaga atgctgatag nacacgccat    480 

accaacttgt ccaacancca ctacagcgat cttattggt                           519 

 
           
             161  
             446  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(446)  
               n = A,T,C or G  
             
           
            161 

cgagnggccc gcccgggcag gtccagtaag cntttnacga tgatgggaaa ggttatgcaa     60 

ggtcccagcg gtacaacgag ctgtttctac atcatttgta ttctgcatgg tacgtacaat    120 

agcagacacc atctgaggag aacgcatgat agcgtgtctg gaagcttcct ttttagaaag    180 

ctgatggacc ataactgcag ccttattaac caccacctgg tcctcgtcat ttagcagttt    240 

tgtcagttca gggattgcac gtgtggcang ttctgcatca tcttgatagt taatcaagtt    300 

tacaactggc atgtttcagc atctgcgatg ggctcagcaa acgctggaca ttantgggat    360 

gagcagcatc aaactgtgta natgggatct gcatgccctc atctaatgtc tcagggaaca    420 

tagcagctcg taccctctga gctcga                                         446 

 
           
             162  
             354  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(354)  
               n = A,T,C or G  
             
           
            162 

agcgtngtcg cggcccgang tcctgggaag cctttnttgc tgagcctcac agcctctgtc     60 

aggcggctgc ggatccagcg gtccaccagg ctctcatggc ctccgggctg ggaggngggt    120 

gagggcacaa aacccttccc aaggccacga anggcaaact tggtggcatt ccanagcttg    180 

ttgcanaagt ggcggnaacc cagtatccgg ttcacatcca ggntgatgtc acgaccctgg    240 

gacatgtang cacataatcc aaaccggaga gcatcggtgc cacattcacg aatccccgct    300 

gggaagtcag ctttctgccc ttctttggcc ttctccacct cgctgggatc cagg          354 

 
           
             163  
             258  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(258)  
               n = A,T,C or G  
             
           
            163 

tttttcncca agtcctcttg ccgngggatc tngactgcaa tttaagacac ttctaattag     60 

ttatacccag gccctgcaaa attgctgggt ttatataata tattcttgct gcacgaagat    120 

ttattattct gttggatgat tctattttaa ttntatttat tctggccaaa aaagaacctt    180 

ctccgctcgt caagagangc caatntgtct tgaaggacaa gagaaagatg ctaacacaca    240 

ctttcttctt cttgagga                                                  258 

 
           
             164  
             282  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(282)  
               n = A,T,C or G  
             
           
            164 

ggaacatatt acttttaaat tacttgggtc aatgaaacat ttaataaaaa catttgcttc     60 

tctatataat acgtatgtat aaaataagcc ttttcanaaa ctctggttct cataatcctc    120 

tataaatcan atgatctgac ttctaagagg aacaaattac agnaaggggt atacattnat    180 

gaatactggt agtactagag ganngacgct aaaccactct actaccactt gcggaactct    240 

cacagggtaa atgacaaagc caatgactga ctctaaaaac aa                       282 

 
           
             165  
             462  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(462)  
               n = A,T,C or G  
             
           
            165 

gcccgggcan gtcctgtaat cccagctact cangangctg agtcatgana atcgcctgaa     60 

tccgggaggt agaggccgca gcgagcaaag attaagccac tgcactccag tctgggtgac    120 

agagtgagaa tctgtctgtt gctcctctgg cattggtctg aaatgggttt gtagaacatg    180 

ccacagaagg accagcanca gcaacaaatg gatttgtgga angcgtagct ccaaatggag    240 

cangcacact tgatgaagca cgctgtgtct gtgcagangc aaccactggc actgttccaa    300 

aaacattgct gctagcatta cttgtggaag tatacgcatt actggaggtg gctgcanaac    360 

tgaaaacgct gtctagttct gccanagctg catacttgnc tgaanatgca cttgactgac    420 

tgggaactga accacanaac caacaggacc tttacctgtg ga                       462 

 
           
             166  
             365  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(365)  
               n = A,T,C or G  
             
           
            166 

cgtgggtcgc ggcncgangt ctgaaaccaa tccagaacta aacatcagca cacaaaaaat     60 

accaggatag atggaatcaa aagactctga agccaaaagg aggctaggga gagcaactga    120 

acttagcaag ctgaggactt cagtgtccat catccgatcc tgccctgtaa caacaggtct    180 

atatgataga gatattccat ctgagctgga ggccattatc cttagcaaac taacacagaa    240 

cagaaaacca aatacatgtt ctcatttaga agtaggagct aaatgatgag aactcaagga    300 

cacaaagaaa ggaacaacag acactggggc ctacttgagg gtggagggtg ggaggaggga    360 

gaaga                                                                365 

 
           
             167  
             364  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(364)  
               n = A,T,C or G  
             
           
            167 

agcgtggtcg cggcgcgang tccagcccta gcttgcctgt gactccgcct tcactgggtg     60 

ctctctctaa aagttgctga ctctttactg tatctcccaa ttcccactcc attggttcca    120 

taaggggagg ggtgtctcac tcaacatggt gttcctggta ccaagaactg gctgacgaag    180 

ctgggtgccg tggctcatgc ctgtaatccc agcacttttg ggaggccaag aagggcggat    240 

cacctgaggt ctggagttca agatcagcct gaccaacatg atgaaaccaa gtctccacta    300 

aaaatataaa acaattagcc aggcatggtg gtgggtgcct gnaatcccag ctactgggga    360 

ngct                                                                 364 

 
           
             168  
             447  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(447)  
               n = A,T,C or G  
             
           
            168 

cccgggcagg tcaaaaccca aaacctttca ttttagccca aaccagctca tgattaggta     60 

tacaaggata acagaaccag ttgtcaggac gagcatttga caagtaaaag caattcttgc    120 

aaagctgcag ttcatccagc tcatggcatg tgtctttata tagcatcctc gcaatgtcag    180 

cttgctcact gtctgctcca tagaaaatca cggtattgtg gagaagcaat tgggcatcag    240 

ctttgaactc ttcataactt cggtatttcc cttcattcac tttctcttga atggtgggaa    300 

cgtccacaga cctcggccgc gaccacgcta agcccgaatt ctgcagatat ccatcacact    360 

ggcggccgtt cgagcatggc atctagaagg cccaattcgc ctatagngag tcgnattacc    420 

aattcactgg ccgtcgnttt acaacgc                                        447 

 
           
             169  
             524  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(524)  
               n = A,T,C or G  
             
           
            169 

cgantngcgc gcccgggcag gtctgagcag cctttctgnn tgctggacta ttgggattgg     60 

gttcatccaa cagagactgt atggatgtta gaatggaaga cacatcatag gttggactcc    120 

aacggttctg aagtatgtcc agacatatac taccatctgc atagactaag aacaaagaag    180 

taggtacatt aaacgtaaca agaccactaa ggttttaaca ttatagacaa aacanaaata    240 

gtcaaganta ctttgctttt gaagtttaaa gattcctatg ttgcttccca gttaactgcc    300 

taaaaagata agncataacc accactagtg aaataatcan gatgatcaga gaatgtcana    360 

tgtgatcagt ataaaactgg angatattna gtgtcatcct ttggaaaagg ctgccctatn    420 

atccaggaaa tcanaaacat tnttgaacag ggnccctagc tatccacaga catgtgggaa    480 

attcattccc caaatngtag gctggatccc ctatctgaaa taac                     524 

 
           
             170  
             332  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(332)  
               n = A,T,C or G  
             
           
            170 

tcgancggcn cgcccgggca ggtgacaaac ctgttattga agatgttggt tctgatgagg     60 

aanaanatca gaagggatgg tgacaagaan aanaanaaga agattaagga aaagtacatc    120 

gatcaagaag agctcaacaa aacaaagccc atctggacca gaaatcccga cgatattact    180 

aatgangagt acggagaatt ctataanagc ttgaccaatg actgggaaga tcacttggca    240 

gtgaagcatt tttcagttga nggacagttg gaattcagag cccttctatn tgtcccacga    300 

cgtgctcctt ttgatctgtt tganancaga aa                                  332 

 
           
             171  
             334  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(334)  
               n = A,T,C or G  
             
           
            171 

cgagnggcnc gcccgggcag gtctgttgat agcgacttaa cagaaaagtc tagacaaaca     60 

taagcataaa aaattacagt ctttctaccc ttgggaatgg ggagaaaaag gaatctctac    120 

cccaagacca gaaataataa gtcctgtttc tggtcctgaa catccagaat tatggaggct    180 

ttggcctgac accacattan aatttggtct ggaaatcaaa ctttaganac angagatcgt    240 

aagccatttt atactatcga cctaaattcc agtctaacgg ttcctttaca aagttgcgga    300 

aagccctctt atatgctagc tgtaggaaat atag                                334 

 
           
             172  
             439  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(439)  
               n = A,T,C or G  
             
           
            172 

agcgtggtcg cggcccgang tctgcctata aaactagact tctgacgctg ggctccagct     60 

tcattctcac aggtcatcat cctcatccgg gagagcagtt gtctgagcaa cctctaagtc    120 

gtgctcatac tgtgctgcca aagctgggtc catgacaact tctggtgggg cgagagcagg    180 

catggcaaca aattccaagt tagggtctcc aatgagcttc ctagcaagcc agaggaaggg    240 

cttttcaaag ttgtagttac ttttggcaga aatgtcgtag tactgaagat tcttctttcg    300 

gtggaagaca atggatttcg ccttcacttt ctgccttaat atccactttg gtgccacaca    360 

acacaatggg gatgntttca cacacttngn accanatctc tatgccagnt aggccatttt    420 

ggaagnactt cganggtac                                                 439 

 
           
             173  
             599  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(599)  
               n = A,T,C or G  
             
           
            173 

cgatnggccg cccgggcagg tcctgtaaaa naggaaattc agacatcgta cgactcgtaa     60 

ttgaatgtgg agctgactgc aatattttgt caaagcacca gaatagtgcc ctgcactttg    120 

cgaagcagtc taacaatgtg cttgtgtacg acttgctgaa gaaccattta gagacacttt    180 

caagagtagc agaagagaca ataaaggatt actttgaagc tcgccttgct ctgctagaac    240 

cagtttttcc aatcgcatgt catcgactct gtgagggtcc agatttttca acagatttca    300 

attaccaacc cccacagaac ataccagaag gctctggcat cctgctgttt atcttccatg    360 

caaacttttt gggtaaagaa gttattgctc ggctctgtgg accgtgtagt gtacaagctg    420 

tagttctgaa tgataaattt cagcttcctg tttttctggg tctcgctctg ttgtccaggc    480 

tggagtgcag tggcgcggat tacagctcac tggagtcttg acttcccagg cacaagcaat    540 

cctcccacct cagcctccta actacctggg actaaaaatg caccgccacc acattccgg     599 

 
           
             174  
             458  
             DNA  
             Homo sapien  
             
               misc_feature  
               (1)...(458)  
               n = A,T,C or G  
             
           
            174 

tcgatttggc cgcccgggca ggtccatgcn gnttntgccc attcccatgg ngcccgacaa     60 

ncccatcccc gaggccgaca tccccatgtt catgttcatg cccaccatgc cctggctcat    120 

ccctgcgctg ttccccagag gggccattcc catggtgccc gtcattacac cgggcatgtt    180 

cataggcatg ggtcccccca ggagagggtt agnttgaggc cggacaggaa gcatgtttga    240 

tggagaactg aggttcacag nctccaaaac tttgagtcat cacattcata ggctgctgca    300 

tattctgtct gctgaatcca ttgtatncag tgatggcctg ctggggnttt ggaaggctng    360 

cataccaggt agtaagntcg tctaggctga tgtttacacc tggggtcaga ccaagtanga    420 

gggcaaggtt ttgctgactg attttctgga cccatatc                            458 

 
           
             175  
             1206  
             DNA  
             Homo sapien  
           
            175 

ggcacgagga agttttgtgt actgaaaaag aaactgtcag aagcaaaaga aataaaatca     60 

cagttagaga accaaaaagt taaatgggaa caagagctct gcagtgtgag gtttctcaca    120 

ctcatgaaaa tgaaaattat ctcttacatg aaaattgcat gttgaaaaag gaaattgcca    180 

tgctaaaact ggaaatagcc acactgaaac accaatacca ggaaaaggaa aataaatact    240 

ttgaggacat taagatttta aaagaaaaga atgctgaact tcagatgacc ctaaaactga    300 

aagaggaatc attaactaaa agggcatctc aatatagtgg gcagcttaaa gttctgatag    360 

ctgagaacac aatgctcact tctaaattga aggaaaaaca agacaaagaa atactagagg    420 

cagaaattga atcacaccat cctagactgg cttctgctgt acaagaccat gatcaaattg    480 

tgacatcaag aaaaagtcaa gaacctgctt tccacattgc aggagatgct tgtttgcaaa    540 

gaaaaatgaa tgttgatgtg agtagtacga tatataacaa tgaggtgctc catcaaccac    600 

tttctgaagc tcaaaggaaa tccaaaagcc taaaaattaa tctcaattat gccggagatg    660 

ctctaagaga aaatacattg gtttcagaac atgcacaaag agaccaacgt gaaacacagt    720 

gtcaaatgaa ggaagctgaa cacatgtatc aaaacgaaca agataatgtg aacaaacaca    780 

ctgaacagca ggagtctcta gatcagaaat tatttcaact acaaagcaaa aatatgtggc    840 

ttcaacagca attagttcat gcacataaga aagctgacaa caaaagcaag ataacaattg    900 

atattcattt tcttgagagg aaaatgcaac atcatctcct aaaagagaaa aatgaggaga    960 

tatttaatta caataaccat ttaaaaaacc gtatatatca atatgaaaaa gagaaagcag   1020 

aaacagaagt tatataatag tataacactg ccaaggagcg gattatctca tcttcatcct   1080 

gtaattccag tgtttgtcac gtggttgttg aataaatgaa taaagaatga gaaaaccaga   1140 

agctctgata cataatcata atgataatta tttcaatgca caactacggg tggtgctgct   1200 

cgtgcc                                                              1206 

 
           
             176  
             317  
             PRT  
             Homo sapien  
           
            176 

Met Gly Thr Arg Ala Leu Gln Cys Glu Val Ser His Thr His Glu Asn 
 1               5                  10                  15 

Glu Asn Tyr Leu Leu His Glu Asn Cys Met Leu Lys Lys Glu Ile Ala 
            20                  25                  30 

Met Leu Lys Leu Glu Ile Ala Thr Leu Lys His Gln Tyr Gln Glu Lys 
        35                  40                  45 

Glu Asn Lys Tyr Phe Glu Asp Ile Lys Ile Leu Lys Glu Lys Asn Ala 
    50                  55                  60 

Glu Leu Gln Met Thr Leu Lys Leu Lys Glu Glu Ser Leu Thr Lys Arg 
65                  70                  75                  80 

Ala Ser Gln Tyr Ser Gly Gln Leu Lys Val Leu Ile Ala Glu Asn Thr 
                85                  90                  95 

Met Leu Thr Ser Lys Leu Lys Glu Lys Gln Asp Lys Glu Ile Leu Glu 
            100                 105                 110 

Ala Glu Ile Glu Ser His His Pro Arg Leu Ala Ser Ala Val Gln Asp 
        115                 120                 125 

His Asp Gln Ile Val Thr Ser Arg Lys Ser Gln Glu Pro Ala Phe His 
    130                 135                 140 

Ile Ala Gly Asp Ala Cys Leu Gln Arg Lys Met Asn Val Asp Val Ser 
145                 150                 155                 160 

Ser Thr Ile Tyr Asn Asn Glu Val Leu His Gln Pro Leu Ser Glu Ala 
                165                 170                 175 

Gln Arg Lys Ser Lys Ser Leu Lys Ile Asn Leu Asn Tyr Ala Gly Asp 
            180                 185                 190 

Ala Leu Arg Glu Asn Thr Leu Val Ser Glu His Ala Gln Arg Asp Gln 
        195                 200                 205 

Arg Glu Thr Gln Cys Gln Met Lys Glu Ala Glu His Met Tyr Gln Asn 
    210                 215                 220 

Glu Gln Asp Asn Val Asn Lys His Thr Glu Gln Gln Glu Ser Leu Asp 
225                 230                 235                 240 

Gln Lys Leu Phe Gln Leu Gln Ser Lys Asn Met Trp Leu Gln Gln Gln 
                245                 250                 255 

Leu Val His Ala His Lys Lys Ala Asp Asn Lys Ser Lys Ile Thr Ile 
            260                 265                 270 

Asp Ile His Phe Leu Glu Arg Lys Met Gln His His Leu Leu Lys Glu 
        275                 280                 285 

Lys Asn Glu Glu Ile Phe Asn Tyr Asn Asn His Leu Lys Asn Arg Ile 
    290                 295                 300 

Tyr Gln Tyr Glu Lys Glu Lys Ala Glu Thr Glu Val Ile 
305                 310                 315 

 
           
             177  
             20  
             DNA  
             Artificial Sequence  
             
               Made in the Lab  
             
           
            177 

ccaatcatct ccacaggagc                                                 20 

 
           
             178  
             1665  
             DNA  
             Homo sapien  
           
            178 

gcaaactttc aagcagagcc tcccgagaag ccatctgcct tcgagcctgc cattgaaatg     60 

caaaagtctg ttccaaataa agccttggaa ttgaagaatg aacaaacatt gagagcagat    120 

cagatgttcc cttcagaatc aaaacaaaag aaggttgaag aaaattcttg ggattctgag    180 

agtctccgtg agactgtttc acagaaggat gtgtgtgtac ccaaggctac acatcaaaaa    240 

gaaatggata aaataagtgg aaaattagaa gattcaacta gcctatcaaa aatcttggat    300 

acagttcatt cttgtgaaag agcaagggaa cttcaaaaag atcactgtga acaacgtaca    360 

ggaaaaatgg aacaaatgaa aaagaagttt tgtgtactga aaaagaaact gtcagaagca    420 

aaagaaataa aatcacagtt agagaaccaa aaagttaaat gggaacaaga gctctgcagt    480 

gtgaggtttc tcacactcat gaaaatgaaa attatctctt acatgaaaat tgcatgttga    540 

aaaaggaaat tgccatgcta aaactggaaa tagccacact gaaacaccaa taccaggaaa    600 

aggaaaataa atactttgag gacattaaga ttttaaaaga aaagaatgct gaacttcaga    660 

tgaccctaaa actgaaagag gaatcattaa ctaaaagggc atctcaatat agtgggcagc    720 

ttaaagttct gatagctgag aacacaatgc tcacttctaa attgaaggaa aaacaagaca    780 

aagaaatact agaggcagaa attgaatcac accatcctag actggcttct gctgtacaag    840 

accatgatca aattgtgaca tcaagaaaaa gtcaagaacc tgctttccac attgcaggag    900 

atgcttgttt gcaaagaaaa atgaatgttg atgtgagtag tacgatatat aacaatgagg    960 

tgctccatca accactttct gaagctcaaa ggaaatccaa aagcctaaaa attaatctca   1020 

attatgccgg agatgctcta agagaaaata cattggtttc agaacatgca caaagagacc   1080 

aacgtgaaac acagtgtcaa atgaaggaag ctgaacacat gtatcaaaac gaacaagata   1140 

atgtgaacaa acacactgaa cagcaggagt ctctagatca gaaattattt caactacaaa   1200 

gcaaaaatat gtggcttcaa cagcaattag ttcatgcaca taagaaagct gacaacaaaa   1260 

gcaagataac aattgatatt cattttcttg agaggaaaat gcaacatcat ctcctaaaag   1320 

agaaaaatga ggagatattt aattacaata accatttaaa aaaccgtata tatcaatatg   1380 

aaaaagagaa agcagaaaca gaaaactcat gagagacaag cagtaagaaa cttcttttgg   1440 

agaaacaaca gaccagatct ttactcacaa ctcatgctag gaggccagtc ctagcattac   1500 

cttatgttga aaatcttacc aatagtctgt gtcaacagaa tacttatttt agaagaaaaa   1560 

ttcatgattt cttcctgaag cctgggcgac agagcgagac tctgtctcaa aaaaaaaaaa   1620 

aaaaaaagaa agaaagaaat gcctgtgctt acttcgcttc ccagg                   1665 

 
           
             179  
             179  
             PRT  
             Homo sapien  
           
            179 

Ala Asn Phe Gln Ala Glu Pro Pro Glu Lys Pro Ser Ala Phe Glu Pro 
 1               5                  10                  15 

Ala Ile Glu Met Gln Lys Ser Val Pro Asn Lys Ala Leu Glu Leu Lys 
            20                  25                  30 

Asn Glu Gln Thr Leu Arg Ala Asp Gln Met Phe Pro Ser Glu Ser Lys 
        35                  40                  45 

Gln Lys Lys Val Glu Glu Asn Ser Trp Asp Ser Glu Ser Leu Arg Glu 
    50                  55                  60 

Thr Val Ser Gln Lys Asp Val Cys Val Pro Lys Ala Thr His Gln Lys 
65                  70                  75                  80 

Glu Met Asp Lys Ile Ser Gly Lys Leu Glu Asp Ser Thr Ser Leu Ser 
                85                  90                  95 

Lys Ile Leu Asp Thr Val His Ser Cys Glu Arg Ala Arg Glu Leu Gln 
            100                 105                 110 

Lys Asp His Cys Glu Gln Arg Thr Gly Lys Met Glu Gln Met Lys Lys 
        115                 120                 125 

Lys Phe Cys Val Leu Lys Lys Lys Leu Ser Glu Ala Lys Glu Ile Lys 
    130                 135                 140 

Ser Gln Leu Glu Asn Gln Lys Val Lys Trp Glu Gln Glu Leu Cys Ser 
145                 150                 155                 160 

Val Arg Phe Leu Thr Leu Met Lys Met Lys Ile Ile Ser Tyr Met Lys 
                165                 170                 175 

Ile Ala Cys 

 
           
             180  
             1681  
             DNA  
             Homo sapien  
           
            180 

gatacagtca ttcttgtgaa agagcaaggg aacttcaaaa agatcactgt gaacaacgta     60 

caggaaaaat ggaacaaatg aaaaagaagt tttgtgtact gaaaaagaaa ctgtcagaag    120 

caaaagaaat aaaatcacag ttagagaacc aaaaagttaa atgggaacaa gagctctgca    180 

gtgtgagatt gactttaaac caagaagaag agaagagaag aaatgccgat atattaaatg    240 

aaaaaattag ggaagaatta ggaagaatcg aagagcagca taggaaagag ttagaagtga    300 

aacaacaact tgaacaggct ctcagaatac aagatataga attgaagagt gtagaaagta    360 

atttgaatca ggtttctcac actcatgaaa atgaaaatta tctcttacat gaaaattgca    420 

tgttgaaaaa ggaaattgcc atgctaaaac tggaaatagc cacactgaaa caccaatacc    480 

aggaaaagga aaataaatac tttgaggaca ttaagatttt aaaagaaaag aatgctgaac    540 

ttcagatgac cctaaaactg aaagaggaat cattaactaa aagggcatct caatatagtg    600 

ggcagcttaa agttctgata gctgagaaca caatgctcac ttctaaattg aaggaaaaac    660 

aagacaaaga aatactagag gcagaaattg aatcacacca tcctagactg gcttctgctg    720 

tacaagacca tgatcaaatt gtgacatcaa gaaaaagtca agaacctgct ttccacattg    780 

caggagatgc ttgtttgcaa agaaaaatga atgttgatgt gagtagtacg atatataaca    840 

atgaggtgct ccatcaacca ctttctgaag ctcaaaggaa atccaaaagc ctaaaaatta    900 

atctcaatta tgccggagat gctctaagag aaaatacatt ggtttcagaa catgcacaaa    960 

gagaccaacg tgaaacacag tgtcaaatga aggaagctga acacatgtat caaaacgaac   1020 

aagataatgt gaacaaacac actgaacagc aggagtctct agatcagaaa ttatttcaac   1080 

tacaaagcaa aaatatgtgg cttcaacagc aattagttca tgcacataag aaagctgaca   1140 

acaaaagcaa gataacaatt gatattcatt ttcttgagag gaaaatgcaa catcatctcc   1200 

taaaagagaa aaatgaggag atatttaatt acaataacca tttaaaaaac cgtatatatc   1260 

aatatgaaaa agagaaagca gaaacagaaa actcatgaga gacaagcagt aagaaacttc   1320 

ttttggagaa acaacagacc agatctttac tcacaactca tgctaggagg ccagtcctag   1380 

cattacctta tgttgaaaaa tcttaccaat agtctgtgtc aacagaatac ttattttaga   1440 

agaaaaattc atgatttctt cctgaagcct acagacataa aataacagtg tgaagaatta   1500 

cttgttcacg aattgcataa aagctgccca ggatttccat ctaccctgga tgatgccgga   1560 

gacatcattc aatccaacca gaatctcgct ctgtcactca ggctggagtg cagtgggcgc   1620 

aatctcggct cactgcaact ctgcctccca ggttcacgcc attctctggc acagcctccc   1680 

g                                                                   1681 

 
           
             181  
             432  
             PRT  
             Homo sapien  
           
            181 

Asp Thr Val His Ser Cys Glu Arg Ala Arg Glu Leu Gln Lys Asp His 
 1               5                  10                  15 

Cys Glu Gln Arg Thr Gly Lys Met Glu Gln Met Lys Lys Lys Phe Cys 
            20                  25                  30 

Val Leu Lys Lys Lys Leu Ser Glu Ala Lys Glu Ile Lys Ser Gln Leu 
        35                  40                  45 

Glu Asn Gln Lys Val Lys Trp Glu Gln Glu Leu Cys Ser Val Arg Leu 
    50                  55                  60 

Thr Leu Asn Gln Glu Glu Glu Lys Arg Arg Asn Ala Asp Ile Leu Asn 
65                  70                  75                  80 

Glu Lys Ile Arg Glu Glu Leu Gly Arg Ile Glu Glu Gln His Arg Lys 
                85                  90                  95 

Glu Leu Glu Val Lys Gln Gln Leu Glu Gln Ala Leu Arg Ile Gln Asp 
            100                 105                 110 

Ile Glu Leu Lys Ser Val Glu Ser Asn Leu Asn Gln Val Ser His Thr 
        115                 120                 125 

His Glu Asn Glu Asn Tyr Leu Leu His Glu Asn Cys Met Leu Lys Lys 
    130                 135                 140 

Glu Ile Ala Met Leu Lys Leu Glu Ile Ala Thr Leu Lys His Gln Tyr 
145                 150                 155                 160 

Gln Glu Lys Glu Asn Lys Tyr Phe Glu Asp Ile Lys Ile Leu Lys Glu 
                165                 170                 175 

Lys Asn Ala Glu Leu Gln Met Thr Leu Lys Leu Lys Glu Glu Ser Leu 
            180                 185                 190 

Thr Lys Arg Ala Ser Gln Tyr Ser Gly Gln Leu Lys Val Leu Ile Ala 
        195                 200                 205 

Glu Asn Thr Met Leu Thr Ser Lys Leu Lys Glu Lys Gln Asp Lys Glu 
    210                 215                 220 

Ile Leu Glu Ala Glu Ile Glu Ser His His Pro Arg Leu Ala Ser Ala 
225                 230                 235                 240 

Val Gln Asp His Asp Gln Ile Val Thr Ser Arg Lys Ser Gln Glu Pro 
                245                 250                 255 

Ala Phe His Ile Ala Gly Asp Ala Cys Leu Gln Arg Lys Met Asn Val 
            260                 265                 270 

Asp Val Ser Ser Thr Ile Tyr Asn Asn Glu Val Leu His Gln Pro Leu 
        275                 280                 285 

Ser Glu Ala Gln Arg Lys Ser Lys Ser Leu Lys Ile Asn Leu Asn Tyr 
    290                 295                 300 

Ala Gly Asp Ala Leu Arg Glu Asn Thr Leu Val Ser Glu His Ala Gln 
305                 310                 315                 320 

Arg Asp Gln Arg Glu Thr Gln Cys Gln Met Lys Glu Ala Glu His Met 
                325                 330                 335 

Tyr Gln Asn Glu Gln Asp Asn Val Asn Lys His Thr Glu Gln Gln Glu 
            340                 345                 350 

Ser Leu Asp Gln Lys Leu Phe Gln Leu Gln Ser Lys Asn Met Trp Leu 
        355                 360                 365 

Gln Gln Gln Leu Val His Ala His Lys Lys Ala Asp Asn Lys Ser Lys 
    370                 375                 380 

Ile Thr Ile Asp Ile His Phe Leu Glu Arg Lys Met Gln His His Leu 
385                 390                 395                 400 

Leu Lys Glu Lys Asn Glu Glu Ile Phe Asn Tyr Asn Asn His Leu Lys 
                405                 410                 415 

Asn Arg Ile Tyr Gln Tyr Glu Lys Glu Lys Ala Glu Thr Glu Asn Ser 
            420                 425                 430