Patent Publication Number: US-PP27051-P3

Title: Zoysia japonica L.×Zoysia tenuifolia L. plant named ‘BK-9’

Description:
Latin name of the genus and species: The Latin name of the genus and species of the novel variety disclosed herein is  Zoysia japonica  L.× Zoysia tenuifolia  L. and therefore characterizes a cross between two species of  Zoysia.    
     Variety denomination: The inventive variety of  Zoysia japonica  L× Zoysia tenuifolia  L. disclosed herein has been given the variety denomination ‘BK-9’. 
     BACKGROUND OF THE INVENTION 
     The present invention relates to a new and distinct perennial variety of  Zoysia japonica  L.× Zoysia tenuifolia  L. 
     ‘BK-9’ (also referred to herein as Geo) is the result of a selection done on a  Zoysia  production field by a sod producer named Craft Farms. The selection was made in 2006. The parent grasses are unknown and therefore are not named. The field which originated ‘BK-9’ was established from progeny selections made in the late 80&#39;s. The progenies were derived from crosses made between  Zoysia japonica  L. and  Zoysia tenuifolia  L. Parents for these crosses were selected from sources that had had good cold hardiness, shade tolerance and overall quality. Plants (progenies) originated from these crosses were selected based on desirable characteristics such as open habit of growth, fine texture and speed of growth. These plants (F2) were re-evaluated and the plants with the best cold hardiness and overall quality were selected and planted on a new field in Southern Alabama. This second field of selections was called ‘BK-7’. In the summer of 2006 a new selection was made on the ‘BK-7’ field based on superior cold hardiness, early spring green up, intense green color, fine texture, high shade tolerance and rapid re-growth after harvesting. This new selection was called ‘BK-9’. After being selected, ‘BK-9’ has been vegetatively propagated in Alabama near Foley. Being a fast lateral growing  Zoysia  grass with fine texture is one of the reasons that makes ‘BK-9’ a unique and different type of  Zoysia ; other characteristics that makes ‘BK-9’ unique are excellent cold hardiness, excellent shade tolerance and improved grass quality. It is anticipated that the plant of this invention will be marketed under the synonym Geo as a trade name. ‘BK-9’ is so identified in pictures and morphological and agronomic charts of this disclosure. 
     SUMMARY OF THE INVENTION 
     ‘BK-9’ is a distinctive variety of  Zoysia japonica  L.× Zoysia tenuifolia  L. having a brilliant green (10GY 5/4 on a Munsell Color Chart), fine leaf and stolon texture, small inflorescence, fast lateral growth, superior low temperature tolerance adaptation and excellent shade tolerance. ‘BK-9’ is a very poor seed producer, making it suitable for vegetative reproduction only, such as sprigs, rhizomes, plantlets, or sod. ‘BK-9’ is adapted for use in the hot-humid, and transition zone areas in the US, zones 6b to 9 of the Plant Heat Zone Map. 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIG. 1 : ‘BK-9’ (Geo) planted in pots at green-house in a research area: Starkville, Miss. 
         FIG. 2 : ‘BK-9’ (Geo) pot for evaluation inside a green-house near Starkville, Miss. 
         FIG. 3 : Leaf detail of ‘BK-9’ (Geo) and other varieties. 
         FIG. 4 : Stolon detail of ‘BK-9’ (Geo) and other varieties. 
         FIG. 5 : Inflorescences of ‘BK-9’ (Geo) and other varieties of  Zoysia.    
         FIG. 6 : ‘BK-9’ field showing flower structures blended in the canopy. 
         FIG. 7 : Test plots with ‘BK-9’ and other experimental  Zoysias  near E. Bernard Tex. 
         FIG. 8 : ‘BK-9’ test plots near E. Bernard, Tex. 
         FIG. 9 : Test plots with ‘BK-9’ near Lexington, Ky. 
     
    
    
     DESCRIPTION OF THE NEW VARIETY 
     The following is a detailed description of the new grass variety, based upon observations of the plant grown in field plots at a research area located near Starkville, Miss. ‘BK-9’ is an outstanding  Zoysia  grass cultivar with fine texture, improved shade tolerance and quick lateral speed of growth. ‘BK-9’ recovers very quickly after harvesting, regenerating a mature field in about half of the time compared to other fine bladed  Zoysias . Its fast recovery from wear make it suited for use on sports fields and golf. ‘BK-9’ has an improved cold hardiness exhibiting little winter damage and winter-kill after the winter, making it suitable for use in the transition zone. ‘BK-9’ has excellent shade tolerance requiring only 3 to 4 hours of sunlight to maintain good quality and color. ‘BK-9’ can only be established vegetatively by sprigs, rhizomes, plugs or sod. Its bright green color (10GY 5/4 on a Munsell Color Chart) and fine texture give it a highly appealing turfgrass quality and appearance. 
     Dimensions of Morphological Structures 
     ‘BK-9’ was compared to 3 other cultivars of  Zoysia  grass in a study planted near Starkville, Miss. starting in August 2010. The three cultivars chosen for comparison were ‘Meyer’, ‘SS-Z15’ and ‘El Toro’ because the parents of ‘BK-9’ are unknown and therefore a comparison to a parent was not possible. 
     Each variety was planted in 1 gallon pots, using potting mix: Redi-earth Plug and Seedling Mix (Sungro Horticulture, Bellevue, Wash.) and were kept at a green-house to produce plant material for morphological evaluations.  FIG. 1  shows ‘BK-9’ and Meyer  Zoysia  as grown in one gallon pots with ‘BK-9’ on the left.  FIG. 2  shows a gallon pot of ‘BK-9’ during evaluation. Plant material was collected using a completely random experiment design with 4 replications (pots). Greenhouse complex had natural sunlight conditions. The pots were allowed to grow for 4 weeks, and then were clipped once to encourage density and stolon development. They were allowed to grow un-mowed for another 4 weeks before measurement. The leaf blade measurements were conducted from Aug. 26-Oct. 7, 2010. Seventy randomly selected leaf blades from each cultivar were measured for length and width. Width was measured at the widest point of the blade. The internode measurements were conducted from Nov. 1-12, 2010. All the stolons that grew off each pot were used to measure the internode diameter and internode length. The inflorescence measurements were conducted from Dec. 14, 2010-Mar. 2, 2011. Twenty five seed-heads were randomly selected from each cultivar. Three florets were randomly selected from each head for measurement. 
     The Analysis of Variance (ANOVA) indicated that cultivars differed significantly for most variables measured (Table 1). 
     
       
         
           
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 Analysis of Variance for Zoysia grass cultivars comparisons with 
               
               
                 ‘13K-9’ conducted during the 2010 and 1011 growing seasons. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                   
                 Mean Squares of the traits 
               
            
           
           
               
               
               
               
               
               
            
               
                 Source 
                 ID (mm) 
                 IL (mm) 
                 LL (mm) 
                 LW (mm) 
                 TIL (mm) 
               
               
                   
               
               
                 Variety 
                 12.1** 
                 1986.9** 
                 20231.6** 
                 1128.4** 
                 34684.1** 
               
               
                 Error 
                 0.09 
                 69.30 
                 316.81 
                 0.203 
                 187.6 
               
               
                 CV 
                 17.18 
                 34.60 
                 23.99 
                 12.63 
                 14.61 
               
               
                   
               
            
           
           
               
               
            
               
                   
                 Mean Squares of the traits 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Source 
                 FRL (mm) 
                 PL (mm) 
                 NSI 
                 SL (mm) 
               
               
                   
               
               
                   
                 Variety 
                 2854.8** 
                 17989.1** 
                 2265.6** 
                 9.42* 
               
               
                   
                 Error 
                 12.28 
                 161.8 
                 21.15 
                 0.06 
               
               
                   
                 CV 
                 13.91 
                 18.56 
                 19.56 
                 8.58 
               
               
                   
               
               
                 ID - Internode Diameter, IL - Internode Length, LL - Leaf Length (mm), LW - Leaf Width (mm), TIL - Total Inflorescence Length, FRL - Floral Region Length, PL - Peduncle Length, NSI - Number of Spikelets per Inflorescence, SL - Spikelet Length. 
               
               
                 *Significant at 5% level 
               
               
                 **Significant at 1% level 
               
            
           
         
       
     
     Cultivars showed significant differences on many of the analyzed morphological characteristics (Table 1). Internode Diameter (ID), Internode Length (IL), Leaf Length (LL), Leaf Width (LW), Total Inflorescence Length (TIL), Floral Region Length (FRL), Peduncle Length (PL) and Number of Spikelets per Inflorescence (NSI) were significantly different at 1% level (Table 1). Spikelet Length (SL) was significantly at 5% level (Table 1). The evaluations were well conducted and produced reliable results as demonstrated by the low Coefficients of Variation (CV) (Table 1). 
     In comparison to ‘Meyer’ and ‘El Toro’, the new variety, ‘BK-9’, has a finer texture because it has narrower leaves, shorter leaves and smaller internode diameter. ‘BK-9’ has a smaller leaf blade length (48.8 mm) when compared to ‘Meyer’ and the other varieties used as comparisons (Table 2). ‘Meyer’ is the variety that looks more similar to ‘BK-9’ using the naked eye, being that both varieties are considered to have a ‘fine texture’ ( FIG. 3 ). ‘Meyer’, ‘SS-Z15’ and ‘El Toro’ have significantly longer leaf blades compared to ‘BK-9’. Leaf blade lengths for ‘Meyer’, ‘SS-Z15’ and ‘El Toro’ were 84.4, 83.4 and 80.1 mm, respectively, while ‘BK-9’ leaf blade length was nearly half (48.8 mm) of those (Table 2). ‘BK-9’ also has the narrower leaf blade width (2 mm) among the tested varieties (Table 2) with ‘Meyer’s&#39; leaf blade width being 3.0 mm and ‘El Toro’&#39;s leaf blade width being 4.2 mm. ‘BK-9’ has a smaller internode diameter (1.4 mm) when compared to ‘Meyer’&#39;s internode diameter (1.6 mm) and when compared to ‘El Toro’&#39;s internode diameter (2.0 mm). Thus, while ‘Meyer’ had the second narrowest leaf blade width (Table 2), it was still significantly different than ‘BK-9’. The leaf blade width difference between the two varieties was 1 mm; which is greater than 0.2 mm, the least significant difference (LSD) at 5% level (Table 2). 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                   Zoysia  cultivar comparisons with ‘BK-9’ conducted during 2010. 
               
            
           
           
               
               
               
            
               
                   
                 Internode 
                 Leaf Blade 
               
            
           
           
               
               
               
               
               
            
               
                 Cultivar 
                 Diameter (mm) 
                 Length (mm) 
                 Width (mm) 
                 Length (mm) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 ‘SS-Z15’ 
                 2.2 
                 25.8 
                 5.1 
                 83.4 
               
               
                 El Toro 
                 2 
                 30.2 
                 4.2 
                 80.1 
               
               
                 ‘BK-9’ 
                 1.4 
                 23.3 
                 2 
                 48.8 
               
               
                 Meyer 
                 1.6 
                 18 
                 3 
                 84.4 
               
               
                 LSD(0.05) 
                 0.1 
                 2.6 
                 0.2 
                 5.9 
               
               
                   
               
            
           
         
       
     
     ‘BK-9’ has a dark purple stolon, similar to ‘El Toro’, while ‘SS-Z15’ and ‘Meyer’ have light green stolons ( FIG. 4 ). Other distinctive characteristics of ‘BK-9’ are its small diameter and short internode. Table 2 shows that among the tested varieties, ‘Meyer’ has the shortest internode length (18.3 mm) and ‘BK-9’ has the second shortest internode length (23.3 mm). The difference between ‘Meyer’ and ‘BK-9’ (5.3 mm) was statistically different at 1% level (Table 1). ‘BK-9’ also exhibits the smallest internode diameter (1.4 mm), when compared to ‘SS-Z15’, ‘El Toro’ and ‘Meyer’ (Table 2). ‘Meyer’ exhibited the second smallest internode diameter (1.6 mm), which was still statistically different from ‘BK-9’ (0.20 mm) at the 5% level (Table 2). 
     Narrow and short leaf blades, combined with small internode diameter and short internode length (Table 2), allow ‘BK-9’ to exhibit a fine and dense aesthetic look. Normally, fine bladed  Zoysias  are spiked to the touch, making it unpleasant to step on or lie on top of. Despite having fine blades and short internodes, ‘BK-9’ is soft to the touch, providing a pleasant feeling when stepped on and touched. 
     Furthermore, the short and narrow leaf blades (Table 2) of ‘BK-9’ results in less scalping when maintained with a rotary mower, when other so called ‘fine bladed’  Zoysias  have to be maintained with a reel mower to avoid scalping, due to their longer and wider leaf blades. 
     In addition, ‘BK-9’ differs from ‘Meyer’ and ‘El Toro’ because it has smaller flowers (smaller total inflorescence length, smaller floral region length and smaller peduncle length) than ‘Meyer’ and ‘El Toro’. ‘BK-9’ has small inflorescences (42.3 mm) when compared to total inflorescence length for ‘Meyer’ and ‘El Toro’ of 90.7 mm and 117.3 mm, respectively. In particular, ‘BK-9’ has short seed-heads (floral region) and peduncles ( FIG. 5 ) compared to other  Zoysia  varieties. ‘BK-9’ floral region length is smaller (11.8 mm) when compared to the floral region length for ‘Meyer’ of 21.9 mm and for ‘El Toro’ of 31.1 mm. Further, ‘BK-9’ has a smaller peduncle length (30.5 mm) when compared to the peduncle length of ‘Meyer’ and ‘El Toro’ of 68.9 mm and 86.1 mm, respectively. 
     The total inflorescence structure of ‘BK-9’ easily hides in the plant canopy, making it difficult to see by a person walking the field.  FIG. 6  shows ‘BK-9’ inflorescences blended into the grass canopy. The result is a grass field having a more pleasant look-with the predominant color being green from the leaves and not the purple color from the floral structure. 
     In addition to having the smallest total inflorescence among the compared varieties, ‘BK-9’ also exhibits the smallest number of spikelets per inflorescence (9.5) and spikelet length (2.3 mm) as shown in Table 3. ‘El Toro’ and ‘SS-Z15’ had a significant longer spikelet: 3.0 and 3.7 mm, respectively; while ‘Meyer’ had a spikelet length (2.6 mm) closer to that of ‘BK-9’. Despite that, the difference between ‘Meyer’ and ‘BK-9’&#39;s spikelet lengths (0.3 mm) was greater than the LSD at 5% (0.1 mm) as shown in Table 3 and therefore is statistically different. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                   Zoysias  inflorescences compared to ‘BK-9’ conducted in 2010. 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Total 
                 Floral 
                   
                   
                   
               
               
                   
                 inflorescence 
                 region 
                 Peduncle 
                 Number of 
                 Spikelet 
               
               
                   
                 length  a   
                 length  b   
                 length  c   
                 spikelets per 
                 length  e   
               
               
                 Cultivar 
                 mm 
                 mm 
                 mm 
                 inflorescence  d   
                 mm 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 ‘SS-Z15’ 
                 124.7 
                 36.0 
                 88.7 
                 25.5 
                 3.7 
               
               
                 El Toro 
                 117.3 
                 31.1 
                 86.1 
                 29.6 
                 3.0 
               
               
                 Meyer 
                 90.7 
                 21.9 
                 68.9 
                 29.4 
                 2.6 
               
               
                 ‘BK-9’ 
                 42.3 
                 11.8 
                 30.5 
                 9.5 
                 2.3 
               
               
                 LSD(0.05) 
                 7.7 
                 2 
                 7.1 
                 2.6 
                 0.1 
               
               
                   
               
               
                   a  Total inflorescence length is the mean of 25 observations per cultivar including the floral region and peduncle. 
               
               
                   b  Floral region length is the mean of 25 observations per cultivar 
               
               
                   c  Peduncle length is the mean of 25 observations per cultivar measured from the base of the floral region to the first node. 
               
               
                   d  Number of spikelets per inflorescence is the mean of 25 observations per cultivar determined by count. 
               
               
                   e  Spikelet length is the mean of 3 observations per inflorescence and 75 per cultivar 
               
            
           
         
       
     
     Growth Habits 
     Lateral Growth 
     A comparison study was installed in a private research company near East Bernard, Tex. in Jul. 15, 2009 to evaluate the speed of lateral growth of ‘BK-9’ and other experimental varieties of  Zoysia  (see,  FIG. 7  and  FIG. 8 ). All entries were planted using plugs produced in 72 cell trays. Plugs were planted in 10×30 ft. plots, using 12 inch centers. Plots were fertilized with 10 lbs. of 10-05-05/1000 sq. ft. spread immediately after planting the plugs, and watered in. Irrigation was applied at least once a week, until plugs were fully grown in, Experimental varieties included: ‘Z-2’, ‘BK-9’, ‘SS-12’, ‘SS-13’, ‘Aloysia’ and ‘SS-Z15’ ( FIG. 7 ). Evaluations of percentage of ground covered (%) by the varieties and visual quality ratings (1 to 9, with 9=highest quality) were collected from July to October of 2009 (Tables 4 and 5). 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Ground cover of experimental Zoysias planted in 2009;  
               
               
                 near E. Bernard,TX. 
               
            
           
           
               
               
            
               
                   
                 Ground cover (%) 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Varieties 
                 8/26 
                 9/5 
                 9/15 
                 9/25 
                 10/6 
                 10/22 
               
               
                   
               
               
                 ‘SS-Z15’ 
                 25 
                 30 
                 30 
                 40 
                 60 
                  85 
               
               
                 Aloysia 
                 30 
                 40 
                 50 
                 85 
                 80 
                  90 
               
               
                 ‘SS 12’ 
                 45 
                 60 
                 90 
                 95 
                 95 
                  97 
               
               
                 ‘SS 13’ 
                 30 
                 50 
                 65 
                 80 
                 90 
                  95 
               
               
                 ‘BK-9’ 
                 40 
                 45 
                 50 
                 80 
                 90 
                 100 
               
               
                 ‘Z-2’ 
                 40 
                 50 
                 60 
                 70 
                 80 
                  95 
               
               
                   
               
               
                 Note: 
               
               
                 Jul. 15, 2009 to Oct. 22, 2009 = 109 days. 
               
            
           
         
       
     
     ‘BK-9’ grows faster than other varieties of  Zoysia . ‘BK-9’ was the first variety to reach 100% of ground cover (Table 4), and the variety with the best average visual quality ratings (8.17) recorded along the tested period (Table 5). Normally, fine bladed  Zoysias  such as ‘Emerald’, ‘Cavalier’ or ‘Diamond’, take 14 months or more to cover the ground after planting. These varieties also take, in average, the same amount of time to recover after harvesting and regenerate a field back to be harvestable again. ‘BK-9’ has demonstrated in a production field situation that can regrow back in 6-8 months after harvesting. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Quality Ratings of experimental Zoysias, 2009,  
               
               
                 planted near E. Bernard, TX. 
               
            
           
           
               
               
            
               
                   
                 Quality Visual Ratings (1-9) 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Varieties 
                 8/26 
                 9/5 
                 9/15 
                 9/25 
                 10/6 
                 10/22 
                 Average 
               
               
                   
               
               
                 ‘SS-Z15’ 
                 6 
                 7 
                 8 
                 6 
                 8 
                 8 
                 7.17 
               
               
                 Aloysia 
                 6 
                 7 
                 8 
                 8 
                 8 
                 8 
                 7.50 
               
               
                 ‘SS 12’ 
                 7 
                 7 
                 7 
                 7 
                 8 
                 8 
                 7.33 
               
               
                 ‘SS 13’ 
                 7 
                 7 
                 6 
                 8 
                 7 
                 8 
                 7.17 
               
               
                 ‘BK-9’ 
                 6 
                 8 
                 9 
                 9 
                 9 
                 8 
                 8.17 
               
               
                 ‘Z-2’ 
                 7 
                 7 
                 7 
                 8 
                 8 
                 8 
                 7.50 
               
               
                   
               
               
                 9 = Best possible rate 
               
            
           
         
       
     
     Fast regrowth is an important, unique and desirable characteristic for a fine bladed  Zoysia . It allows ‘BK-9’ to be produced on average, more than once a year; reducing its production cost and as a consequence making the market price more affordable for the end user. The fact that ‘BK-9’ grows from plugs to 100% cover ( FIG. 8 ), in 109 days (Table 4), indicates that it can regenerate a production field after harvesting more quickly than other fine bladed  Zoysias.    
     Cold Tolerance 
     ‘BK-9’ has less winter kill and faster spring green up when compared to other varieties of  Zoysia . In order to evaluate the cold tolerance of ‘BK-9’ and the amount of winter damage it sustain when used in the transition zone of the United States (Zone 7 and 6b USDA Plant Hardiness Zone Map), a replica of the experiment planted in central Texas was planted in a sod producer area near Raeford, N.C. (Zone 7) and in a private research company near Lexington, Ky. (Zone 6b) (see,  FIG. 9 ). Test plots were the same size as in Texas (10×30 ft.), planted with plugs using the same methodology, i.e. 12 inches center and identical fertilization regime. Test plots in North Carolina were planted in April 2009 and were planted in June 2009 in Kentucky ( FIG. 9 ). 
     Test plots near Lexington, Ky. provided the best information about cold hardiness and winter damage on ‘BK-9’ because the research was located on the southern part of Hardiness Zone 6, with average minimum temperatures ranging for −10 to 0 F. Plots were planted on late spring of 2009 and maintained throughout the growing season with good nutrition and irrigation. After the 2009/2010 winter, plots were evaluated with visual ratings for % of winter kill, spring green up and overall quality (Table 6). 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Evaluations on experimental  Zoysias  planted near Lexington, KY. 
               
            
           
           
               
               
            
               
                   
                 Visual Ratings 
               
            
           
           
               
               
               
               
            
               
                 Varieties 
                 Winter kill (%) 
                 Spring Green-up (1-9) 
                 Overall Quality (1-9) 
               
               
                   
               
               
                 ‘SS-Z15’ 
                 5 
                 5 
                 5 
               
               
                 Aloysia 
                 7 
                 6 
                 6 
               
               
                 ‘SS 12’ 
                 4 
                 3 
                 4 
               
               
                 ‘SS 13’ 
                 2 
                 4 
                 4 
               
               
                 ‘BK-9’ 
                 8 
                 7 
                 8 
               
               
                 ‘Z-2’ 
                 4 
                 4 
                 5 
               
               
                   
               
               
                 9 = Best possible rate 
               
            
           
         
       
     
     Results showed that ‘BK-9’ exhibit the least winter kill (8/9), the best spring green-up (7/9) and overall quality (8/9) among the tested varieties (Table 6).  FIG. 9  shows the evolution of two entries planted near Lexington, Ky.: ‘BK-9’ on the left column and ‘SS-12’ on the right column. The first row shows entries right after planting on June 2009, the second row shows pictures of the entries taken in September 2009 and the third and last row are pictures of the entries taken in the following summer, June 2010. Both entries (‘BK-9’ and ‘SS-12’) had a good establishment (first row of  FIG. 9 ) and development during the growing season (second row of  FIG. 9 ). However, after the 2009/2010 winter, the ‘SS-12’ entry suffered substantial winter kill (4/9—Table 6) while ‘BK-9’ had close to zero winter kill (8/9—Table 6). In addition, ‘BK-9’ had an early and uniform green-up (7/9—Table 6) compared to ‘SS-12’ (3/9—Table 6); resulting in a better overall quality for ‘BK-9’ (8/9—Table 6) over ‘SS-12’ (4/8—Table 6). The low winter kill, uniform green-up and best overall quality of ‘BK-9’ can be visualized on the third and last row of  FIG. 9 .