Patent Publication Number: US-2010113393-A1

Title: Boronic Acid Salts

Description:
This application is a continuation of U.S. application Ser. No. 11/980,398, filed Oct. 31, 2007, which is a divisional of U.S. application Ser. No. 10/659,178, filed Oct. 9, 2003, each of which are hereby incorporated by reference in their entireties. 
    
    
     BACKGROUND 
     The present disclosure relates to pharmaceutically useful products obtainable from organoboronic acids. The disclosure also relates to the use of members of the aforesaid class of products, to their formulation, their preparation, their synthetic intermediates and to other subject matter. 
     The disclosure further relates to oral pharmaceutical formulations containing the described products. 
     Boronic Acid Compounds 
     It has been known for some years that boronic acid compounds and their derivatives, e.g. esters, have biological activities, notably as inhibitors or substrates of proteases. For example, Koehler et al.  Biochemistry  10:2477, 1971 report that 2-phenylethane boronic acid inhibits the serine protease chymotrypsin at millimolar levels. The inhibition of chymotrypsin and subtilisin by arylboronic acids (phenylboronic acid, m-nitro-phenylboronic acid, m-aminophenylboronic acid, m-bromophenylboronic acid) is reported by Phillip et al,  Proc. Nat. Acad. Sci. USA  68:478-480, 1971. A study of the inhibition of subtilisin Carlsberg by a variety of boronic acids, especially phenyl boronic acids substituted by Cl, Br, CH 3 , H 2 N, MeO and others, is described by Seufer-Wasserthal et al,  Biorg. Med. Chem.  2(1):35-48, 1994. 
     In describing inhibitors or substrates of proteases, P1, P2, P3, etc. designate substrate or inhibitor residues which are amino-terminal to the scissile peptide bond, and S1, S2, S3, etc., designate the corresponding subsites of the cognate protease in accordance with: Schechter, I. and Berger, A. On the Size of the Active Site in Proteases,  Biochem.Biophys.Res.Comm.,  27:157-162, 1967. In thrombin, the S1 binding site or “specificity pocket” is a well defined slit in the enzyme, whilst the S2 and S3 binding subsites (also respectively called the proximal and distal hydrophobic pockets) are hydrophobic and interact strongly with, respectively, Pro and (R)-Phe, amongst others. 
     Pharmaceutical research into serine protease inhibitors has moved from the simple arylboronic acids to boropeptides, i.e. peptides containing a boronic acid analogue of an α-amino carboxylic acid. The boronic acid may be derivatised, often to form an ester. Shenvi (EP-A-145441 and U.S. Pat. No. 4,499,082) disclosed that peptides containing an α-aminoboronic acid with a neutral side chain were effective inhibitors of elastase and has been followed by numerous patent publications relating to boropeptide inhibitors of serine proteases. Specific, tight binding boronic acid inhibitors have been reported for elastase (K i , 0.25 nM), chymotrypsin (K i , 0.25 nM), cathepsin G (K i , 21 nM), α-lytic protease (K i , 0.25 nM), dipeptidyl aminopeptidase type IV (K i , 16 pM) and more recently thrombin (Ac-D-Phe-Pro-boroArg-OH (DuP 714 Initial K i , 1.2 nM). 
     Claeson et al (U.S. Pat. No. 5,574,014 and others) and Kakkar et al (WO 92/07869 and family members Including U.S. Pat. No. 5,648,338) disclose thrombin inhibitors having a neutral C-terminal side chain, for example an alkyl or alkoxyalkyl side chain. 
     Modifications of the compounds described by Kakkar et al are included in WO 96/25427, directed to peptidyl serine protease inhibitors in which the P2-P1 natural peptide linkage is replaced by another linkage. As examples of non-natural peptide linkages may be mentioned —CO 2 —, —CH 2 O—, —NHCO—, —CHYCH 2 —, —CH═CH—, —CO(CH 2 ) p CO— where p is 1, 2 or 3, —COCHY—, —CO 2 —CH 2 NH—, —CHY—NX—, —N(X) CH 2 —N(X) CO—, —CH═C(CN)CO—, —CH(OH)—NH—, —CH(CN)—NH—, —CH(OH)—CH 2 — or —NH—CHOH—, where X is H or an amino protecting group and Y is H or halogen, especially F. Particular non-natural peptide linkages are —CO 2 — or —CH 2 O—. 
     Metternich (EP 471651 and U.S. Pat. No. 5,288,707, the latter being assigned to Trigen Limited) discloses variants of Phe-Pro-BoroArg boropeptides in which the P3 Phe is replaced by an unnatural hydrophobic amino acid such as trimethylsilylalanine, p-tert.butyl-diphenyl-silyloxymethylphenylalanine or p-hydroxymethylphenylalanine and the P1 side chain may be neutral (alkoxyalkyl, alkylthioalkyl or trimethylsilylalkyl). 
     The replacement of the P2 Pro residue of borotripeptide thrombin inhibitors by an N-substituted glycine is described in Fevig J M et al  Bioorg. Med. Chem.  8: 301-306 and Rupin A et al  Thromb. Haemost.  78(4):1221-1227, 1997. See also U.S. Pat. No. 5,585,360 (de Nanteuil et al). 
     Amparo (WO 96/20698 and family members including U.S. Pat. No. 5,698,538) discloses peptidomimetics of the structure Aryl-linker-Boro(Aa), where Boro(Aa) may be an aminoboronate residue with a non-basic side chain, for example BoroMpg. The linker is of the formula —(CH 2 ) m CONR— (where m is 0 to 8 and R is H or certain organic groups) or analogues thereof in which the peptide linkage —CONR— is replaced by —CSNR—, —SO 2 NR—, —CO 2 —, —C(S)O— or —SO 2 O—. Aryl is phenyl, naphthyl or biphenyl substituted by one, two or three moieties selected from a specified group. Most typically these compounds are of the structure Aryl-(CH 2 ) n —CONH—CHR 2 —BY 1 Y 2 , where R 2  is for example a neutral side chain as described above and n is 0 or 1. 
     Non-peptide boronates have been proposed as inhibitors of proteolytic enzymes in detergent compositions. WO 92/19707 and WO 95/12655 report that arylboronates can be used as inhibitors of proteolytic enzymes in detergent compositions. WO 92/19707 discloses compounds substituted meta to the boronate group by a hydrogen bonding group, especially acetamido (—NHCOCH 3 ), sulfonamido (—NHSO 2 CH 3 ) and alkylamino. WO 95/12655 teaches that ortho-substituted compounds are superior. 
     Boronate enzyme inhibitors have wide application, from detergents to bacterial sporulation inhibitors to pharmaceuticals. In the pharmaceutical field, there is patent literature describing boronate inhibitors of serine proteases, for example thrombin, factor Xa, kallikrein, elastase, plasmin as well as other serine proteases like prolyl endopeptidase and Ig AI Protease. Thrombin is the last protease in the coagulation pathway and acts to hydrolyse four small peptides form each molecule of fibrinogen, thus deprotecting its polymerisation sites. Once formed, the linear fibrin polymers may be cross-linked by factor XIIIa, which is itself activated by thrombin. In addition, thrombin is a potent activator of platelets, upon which it acts at specific receptors. Thrombin also potentiates its own production by the activation of factors V and VIII. 
     Other aminoboronate or peptidoboronate inhibitors or substrates of serine proteases are described in: 
     U.S. Pat. No. 4,935,493 
     EP 341661 
     WO 94/25049 
     WO 95/09859 
     WO 96/12499 
     WO 96/20689 
     Lee S-L et al, Biochemistry 36:13180-13186, 1997 
     Dominguez C et al  Bioorg. Med. Chem. Lett.  7:79-84, 1997 
     EP 471651 
     WO 94/20526 
     WO 95/20603 
     W097/05161 
     U.S. Pat. No. 4,450,105 
     U.S. Pat. No. 5,106,948 
     U.S. Pat. No. 5,169,841. 
     Peptide boronic acid inhibitors of hepatic C. virus protease are described in WO 01/02424. 
     Matteson D S  Chem, Rev.  89: 1535-1551, 1989 reviews the use of α-halo boronic esters as intermediates for the synthesis of inter alia amino boronic acids and their derivatives, Matteson describes the use of pinacol boronic esters in non-chiral synthesis and the use of pinanediol boronic esters for chiral control, including in the synthesis of amino and amido boronate esters. 
     Contreras et al  J. Organomet. Chem.  246: 213-217, 1983 describe how intramolecular N→B coordination was demonstrated by spectroscopic studies on cyclic boronic esters prepared by reacting Me 2 CHCMe 2 -BH 2  with diethanolamines. 
     Boronic acid and ester compounds have displayed promise as inhibitors of the proteasome, a multicatalytic protease responsible for the majority of intracellular protein turnover. Ciechanover,  Cell,  79:13-21, 1994, teaches that the proteasome is the proteolytic component of the ubiquitinproteasome pathway, in which proteins are targeted for degradation by conjugation to multiple molecules of ubiquitin. Clechanover also teaches that the ubiquitin-proteasome pathway plays a key role in a variety of important physiological processes. 
     Adams et al, U.S. Pat. No. 5,780,454 (1998), U.S. Pat. No. 6,066,730 (2000), U.S. Pat. No. 6,083,903 (2000) and equivalent WO 96/13266, and U.S. Pat. No. 6,297,217 (2001) describe peptide boronic ester and acid compounds useful as proteasome inhibitors. These documents also describe the use of boronic ester and acid compounds to reduce the rate of muscle protein degradation, to reduce the activity of NF- K B in a cell, to reduce the rate of degradation of p53 protein in a cell, to inhibit cyclin degradation in a cell, to inhibit the growth of a cancer cell, to inhibit antigen presentation in a tell, to inhibit NF- K B dependent cell adhesion, and to inhibit HIV replication. Brand et al, WO 98/35691, teaches that proteasorne inhibitors, including boronic acid compounds, are useful for treating infarcts such as occur during stroke or myocardial infarction. Elliott et al, WO 99/15183, teaches that proteasome inhibitors are useful for treating inflammatory and autoimmune diseases. 
     Unfortunately, organoboronic acids can be relatively difficult to obtain in analytically pure form. Thus, alkylboronic acids and their boroxines are often air-sensitive. Korcek et al,  J. Chem. Soc. Perkin Trans  2:242, 1972, teaches that butylboronic acid is readily oxidized by air to generate 1-butanol and boric acid. 
     It is known that derivatisation of boronic acids as cyclic esters provides oxidation resistance. For example, Martichonok V et al  J. Am. Chem. Soc.  118: 950-958, 1996 state that diethanolamine derivatisation provides protection against possible boronic acid oxidation. 
     Wu et al,  J. Pharm. Sci,  89:758-765, 2000, discuss the stability of the compound N-(2-pyrazine) carbonyl-phenylalanine-leucine boronic acid (LDP-341, also known as bortezomib), an anti-cancer agent. It is described how “during an effort to formulate [LDP-341] for parenteral administration, the compound showed erratic stability behaviour”. The degradation pathways were investigated and it was concluded that the degradation was oxidative, the initial oxidation being attributed to peroxides or molecular oxygen and its radicals. 
     WO 02/059131 discloses boronic add products which are described as stable. In particular, these products are certain boropeptides and/or boropeptidomimetics in which the boronic acid group has been derivatised with a sugar. The disclosed sugar derivatives, which have hydrophobic amino acid side chains, are of the formula 
     
       
         
         
             
             
         
       
     
     wherein: 
     P is hydrogen or an amino-group protecting moiety; 
     R is hydrogen or alkyl; 
     A is 0, 1 or 2; 
     R 1 , R 2  and R 3  are independently hydrogen, alkyl, cycloalkyl, aryl or —CH 2 —R 5 ; 
     R 5 , in each instance, is one of aryl, aralkyl, alkaryl, cycloalkyl, heterocyclyl, heteroaryl, or —W—R 6 , where W is a chalcogen and R 6  is alkyl; 
     where the ring portion of any of said aryl, aralkyl, alkaryl, cycloalkyl, heterocyclyl, or heteroaryl in R 1 , R 2 , R 3  or R 5  can be optionally substituted; and 
     Z 1  and Z 2  together form a moiety derived from a sugar, wherein the atom attached to boron in each case is an oxygen atom. 
     Some of the disclosed compounds are sugar derivatives of LDP-341 (see above). 
     Many drugs comprise an active moiety which is a carboxylic acid. There are a number of differences between carboxylic acids and boronic acids, whose effects on drug delivery, stability and transport (amongst others) have not been investigated. One feature of trivalent boron compounds is that the boron atom is sp 2  hybridised, which leaves an empty 2p z  orbital on the boron atom. A molecule of the type BX 3  can therefore act as an electron-pair acceptor, or Lewis acid. It can use the empty 2p z , orbital to pick up a pair of nonbonding electrons from a Lewis base to form a covalent bond. BF 3  therefore reacts with Lewis bases such as NH 3  to form acid-base complexes in which all of the atoms have a filled shell of valence electrons. 
     Boric acid, accordingly, can act as a Lewis acid, accepting OH − : 
       B(OH) 3 +H 2 O→B(OH) 4   − +H +   
     Further, boronic acids of the type RB(OH) 2  are dibasic and have two pKa&#39;s. Another point of distinction about boron compounds is the unusually short length of bonds to boron, for which three factors may be responsible:
         1. Formation of pπ-pπ bonds;   2. Ionic-covalent resonance;   3. Reduced repulsions between non-bonding electrons.       

     The presumed equilibria of boronic and carboxylic acids in aqueous KOH are shown below (excluding formation of RBO 2   2 ): 
     
       
         
         
             
             
         
       
     
     Thrombosis 
     Hemostasis is the normal physiological condition of blood in which its components exist in dynamic equilibrium. When the equilibrium is disturbed, for instance following injury to a blood vessel, certain biochemical pathways are triggered leading, in this example, to arrest of bleeding via clot formation (coagulation). Coagulation is a dynamic and complex process in which proteolytic enzymes such as thrombin play a key role. Blood coagulation may occur through either of two cascades of zymogen activations, the extrinsic and intrinsic pathways of the coagulation cascade. Factor VIIa in the extrinsic pathway, and Factor IXa in the intrinsic pathway are important determinants of the activation of factor X to factor Xa, which itself catalyzes the activation of prothrombin to thrombin, whilst thrombin in turn catalyses the polymerization of fibrinogen monomers to fibrin polymer. The last protease in each pathway is therefore thrombin, which acts to hydrolyze four small peptides (two FpA and two FpB) from each molecule of fibrinogen, thus deprotecting its polymerization sites. Once formed, the linear fibrin polymers may be cross-linked by factor XIIIa, which is itself activated by thrombin. In addition, thrombin is a potent activator of platelets, upon which it acts at specific receptors. Thrombin activation of platelets leads to aggregation of the cells and secretion of additional factors that further accelerate the creation of a hemostatic plug. Thrombin also potentiates its own production by the activation of factors V and VIII (see Hemker and Beguin in: Jolles, et. al., “Biology and Pathology of Platelet Vessel Wall Interactions,” pp. 219-26 (1986), Crawford and Scrutton in: Bloom and Thomas, “Haemostasis and Thrombosis,” pp. 47-77, (1987), Bevers, et. al.,  Eur. J. Biochem.  122:429-36, 1982, Mann,  Trends Biochem. Sci.  12:229-33, 1987). 
     Proteases are enzymes which cleave proteins at specific peptide bonds. Cuypers et al.,  J. Blol. Chem.  257:7086, 1982, and the references cited therein, dassify proteases on a mechanistic basis into five classes: serine, cysteinyl or thiol, acid or aspartyl, threonine and metalloproteases. Members of each class catalyse the hydrolysis of peptide bonds by a similar mechanism, have similar active site amino acid residues and are susceptible to class-specific inhibitors. For example, all serine proteases that have been characterised have an active site serine residue. 
     The coagulation proteases thrombin, factor Xa, factor VIIa, and factor IXa are serine proteases having trypsin-like specificity for the cleavage of sequence-specific Arg-Xxx peptide bonds. As with other serine proteases, the cleavage event begins with an attack of the active site serine on the scissile bond of the substrate, resulting in the formation of a tetrahedral intermediate. This is followed by collapse of the tetrahedral intermediate to form an acyl enzyme and release of the amino terminus of the cleaved sequence. Hydrolysis of the acyl enzyme then releases the carboxy terminus. 
     As indicated above, platelets play two important roles in normal hemostasis. First, by aggregating, they constitute the initial hemostatic plug which immediately curtails bleeding from broken blood vessels. Secondly, the platelet surface can become activated and potentiate blood clotting, a property referred to as platelet procoagulant activity. This may be observed as an increase in the rate of activation of prothrombin by factor Xa In the presence of factor Va and Ca 2+ , referred to as the prothrombinase reaction. Normally, there are few (if any) clotting factors on the surface of unstimulated platelets but, when platelets are activated, negatively charged phospholipids (phosphatidylserine and phospatidylinositol) that are normally on the cytoplasmic side of the membrane become available and provide a surface on which two steps of the coagulation sequence occur. The phospholipid on the surface of activated platelets profoundly accelerates the reactions leading to the formation of thrombin, so that thrombin can be generated at a rate faster than its neutralisation by antithrombin III. The reactions that occur on the platelet surfaces are not easily inhibited by the natural anticoagulants in blood such as antithrombin III, either with or without heparin. (See Kelton and Hirsch in: Bloom and Thomas, “Haemostasis and Thrombosis,” pp. 737-760, (1981); Mustard et al in: Bloom and Thomas, “Haemostasis and Thrombosis,” pp. 503526, (1981); Goodwin et al; Biochem. J. 308:15-21, 1995). 
     A thrombus can be considered as an abnormal product of a normal mechanism and can be defined as a mass or deposit formed from blood constituents on a surface of the cardiovascular system, for example of the heart or a blood vessel. Thrombosis can be regarded as the pathological condition wherein improper activity of the hemostatic mechanism results In intravascular thrombus formation. Three basic types of thrombi are recognised:
         the white thrombus which is usually seen in arteries and consists chiefly of platelets;   the red thrombus which is found in veins and is composed predominantly of fibrin and red cells;   the mixed thrombus which is composed of components of both white and red thrombi.       

     The composition of thrombi is influenced by the velocity of blood flow at their sites of formation. In general white platelet-rich thrombi form in high flow systems, while red coagulation thrombi form in regions of stasis. The high shear rate In arteries prevents the accumulation of coagulation intermediates on the arterial side of the circulation: only platelets have the capacity to form thrombi binding to the area of damage via von Willebrand factor. Such thrombi composed only of platelets are not stable and disperse. If the stimulus is strong then the thrombi will form again and then disperse continually until the stimulus has diminished. For the thrombus to stabilise, fibrin must form. In this respect, small amounts of thrombin can accumulate within the platelet thrombus and activate factor Va and stimulate the platelet procoagulant activity. These two events lead to an overall increase in the rate of activation of prothrombin by factor Xa of 300,000 fold. Fibrin deposition stabilises the platelet thrombus. Indirect thrombin inhibitors, for example heparin, are not dinically effective at inhibiting stimulation of platelet procoagulant activity. Accordingly, a therapeutic agent which inhibits platelet procoagulant activity would be useful for treating or preventing arterial thrombotic conditions. 
     On the venous side of circulation, the thrombus is comprised of fibrin: thrombin can accumulate because of the slower flow on the venous side and platelets play only a minor role. 
     Thrombosis is thus not considered to be a single indication but, rather, is a class of indications embracing distinct sub-classes for which differing therapeutic agents and/or protocols may be appropriate. Thus, regulatory authorities treat disorders such as, for example, deep vein thrombosis, cerebrovascular arterial thrombosis and pulmonary embolism as distinct indications for the purposes of licensing medicines. Two main sub-classes of thrombosis are arterial thrombosis and venous thrombosis. Arterial thrombosis includes such specific disorders as acute coronary syndromes [for example acute myocardial infarction (heart attack, caused by thrombosis in a coronary artery)], cerebrovascular arterial thrombosis (stroke, caused by thrombosis in the cerebrovascular arterial system) and peripheral arterial thrombosis. Examples of conditions caused by venous thrombosis are deep vein thrombosis and pulmonary embolism. 
     The management of thrombosis commonly involves the use of antiplatelet drugs (inhibitors of platelet aggregation) to control future thrombogenesis and thrombolytic agents to lyse the newly formed clot, either or both such agents being used in conjunction or combination with anticoagulants. Anticoagulants are used also preventatively (prophylactically) in the treatment of patients thought susceptible to thrombosis. 
     Currently, two of the most effective classes of drugs in clinical use as anticoagulants are the heparins and the vitamin K antagonists. The heparins are ill-defined mixtures of sulfated polysaccharides that bind to, and thus potentiate, the action of antithrombin III, Antithrombin III is a naturally occurring inhibitor of the activated clotting factors IXa, Xa, XIa, thrombin and probably XIIa (see Jaques,  Pharmacol. Rev.  31:99-166, 1980). The vitamin K antagonists, of which warfarin is the most well-known example, act indirectly by inhibiting the post-ribosomal carboxylations of the vitamin K dependent coagulation factors II, VII, IX and X (see Hirsch,  Semin. Thromb. Hemostasis  12:1-11, 1986). While effective therapies for the treatment of thrombosis, heparins and vitamin K antagonists have the unfortunate side effects of bleeding, heparin-induced thrombocytopenla (in the case of heparin) and marked interpatient variability, resulting in a small and unpredictable therapeutic safety margin. 
     The use of direct acting inhibitors of thrombin and other serine protease enzymes of the coagulation system is expected to alleviate these problems, To that end, a wide variety of serine protease inhibitors have been tested, including boropeptides, i.e. peptides containing a boronic acid analogue of an a-amino acid. Whilst direct acting boronic acid thrombin inhibitors have been discussed earlier in this specification, they are further described in the following section. 
     Neutral PI Residue Boropeptide Thrombin Inhibitors 
     Claeson et al (U.S. Pat. No. 5,574,014 and others) and Kakkar et al (WO 92/07869 and family members including U.S. Pat. No. 5,648,338) disclose lipophilic thrombin inhibitors having a neutral (uncharged) C-terminal (P1) side chain, for example an alkoxyalkyl side chain. 
     The Claeson et al and Kakkar et al patent families disclose boronate esters containing the amino acid sequence D-Phe-Pro-BoroMpg [(R)-Phe-Pro-BoroMpg], which are highly specific inhibitors of thrombin. Of these compounds may be mentioned in particular Cbz-(R)-Phe-Pro-BoroMpg-OPinacol (also known as TRI 50b). The corresponding free boronic acid is known as TRI 50c. For further information relating to TRI 50b and related compounds, the reader is referred to the following documents:
         Elgendy S et al., in  The Design of Synthetic Inhibitors of Thrombin , Claeson G et al Eds,  Advances in Experimental Medicine,  340:173-178, 1993.   Ciaeson G et al,  Biocheml.  290:309-312, 1993   Tapparelli C et al,  J. Biol. Chem.,  268:4734-4741, 1993   Claeson G, in  The Design of Synthetic Inhibitors of Thrombin , Claeson G et al Eds,  Advances in Experimental Medicine,  390:83-91, 1993   Phillip et al, in  The Design of Synthetic Inhibitors of Thrombin , Claeson G et al Eds,  Advances in Experimental Medicine,  340:67-77, 1993   Tapparelli C et al,  Trends Pharmacol. Sci.  14:366-376, 1993   Claeson G,  Blood Coagulation and Fibrinolysis  5:411-436, 1994   Elgendy et al,  Tetrahedron  50:3803-3812, 1994   Deadman 3 et al,  J. Enzyme Inhibition  9:29-41, 1995   Deadman 3 et al,  J. Medicinal Chemistry  38:1511-1522, 1995.       

     The tripeptide sequence of TRI 50b has three chiral centres. The Phe residue is considered to be of (R)-configuration and the Pro residue of natural (S)-configuration, at least in compounds with commercially useful inhibitor activity; the Mpg residue is believed to be of (R)-configuration in isomers with commercially useful Inhibitor activity. Thus, the active, or most active, TRI 50b stereoisomer is considered to be of R,S,R configuration and may be represented as: 
     
       
         
         
             
             
         
       
     
     Whilst indirect acting thrombin inhibitors have been found useful for the treatment of patients susceptible to or suffering from venous thrombosis, the same is not true of arterial thrombosis, because it would be necessary to raise the dosage used in the treatment of venous thrombosis by many times in order to treat (prevent) arterial thrombosis. Such raised dosages typically cause bleeding, which makes indirect acting thrombin inhibitors unsuitable or less preferable for treating arterial thrombosis. Heparin and its low molecular weight derivatives are indirect thrombin inhibitors, and so are unsuitable to treat arterial thrombosis. Oral direct thrombin inhibitors are in development for arterial indications but may have lower than desirable therapeutic indices, i.e. may have higher than desirable levels of bleeding at therapeutic doses. 
     Many organoboronic acid compounds may be classified as lipophilic or hydrophobic. Typically, such compounds include amongst others:
         boropeptides of which all or a majority of the amino acids are hydrophobic   boropeptides of which at least half of the amino acids are hydrophobic and which have a hydrophobic N-terminal substituent (amino protecting group)   non-peptides based on hydrophobic moieties.       

     Oral Absorption 
     Absorption in the gastro-intestinal tract can be by an active or a passive route. Active absorption by transport mechanisms tends to be variable between individuals and with intestinal content (Gustafsson et al,  Thrombosis Research,  2001, 101, 171-181). The upper intestine has been identified as the principal site of oral drug absorption, In particular, the duodenum is the customary target site for absorption of orally administered drugs because of its large surface area. The intestinal mucosa acts as a barrier that controls passive transcellular absorption: the absorption of ionic species Is blocked whilst the transcellular absorption of lipophilic molecules is favoured (Palm K et al.,  J. Pharmacol and Exp. Therapeutics,  1999, 291, 435-443). 
     Orally administered drugs are required to be consistently and adequately absorbed. Variability of absorption between individuals or between different occasions in the same individual is unwelcome. Similarly, drugs which have a low level of bioavailability (only a small portion of the administered active agent is absorbed) are generally unacceptable. 
     Non-ionised compounds are favoured for passive absorption, a route associated with invariability, and are therefore preferred for consistent absorption. Lipophilic species are particularly favoured by passive absorption mechanisms and, accordingly, non-ionic, lipophilic drugs are indicated to be most favoured for consistent and high oral absorption. 
     Typical functionalities required for interaction of drugs with their physiological targets are functional groups such as carboxylic and sulphonic acids. These groups exist as the protonated form in the stomach (at pH 2-3), but will be ionised to some extent at the higher pH of the Intestinal fluid. One strategy that has been used to avoid the ionisation of the carboxylates or sulphonates is to present them as ester forms, which are cleaved once absorbed into the vascular lumen. 
     For example, the direct acting thrombin inhibitor rnelagatran, which has sub-optimal gastrointestinal absorption, has terminal carboxy and amidino groups and is a pure zwitterion at pH 8-10 when the carboxylic acid and amidino groups are both charged. A prodrug H 376/95 was therefore developed which has protecting groups for the carboxylic acid and for the amidine and is a more lipophilic molecule than melagatran. The prodrug has a permeability coefficient across cultured epithelial Caco-2 cells 80 times higher than that of melagatran and oral bioavailability 2.7-5.5 times higher than that of melagatran as well as much smaller variability in the area under the drug plasma concentration vs. time curve (Gustafsson et al,  Thrombosis Research,  2001, 101, 171-181). 
     Oral Absorption of Boropeptides, Boropeptidomimetics and Other Organoboronates 
     The boronate ester group of TRI 50b is rapidly cleaved in the conditions of the plasma to form the corresponding boronic acid group, which is considered to be the active moiety which inhibits the catalytic site of thrombin. 
     Boronic acids are divalent functional groups, with boron-oxygen bond lengths (1.6 Å) more typical of single bonds, unlike superficially comparable C—O and S—O bonds in carboxylic and sulphonic adds. Consequently the boronic acid group has two ionisation potentials. The boronic acid group will be partly ionised at pH&#39;s of the duodenal fluid and not suited to the desired passive duodenal uptake. Thus, a charged boronate inhibitor H-D-PheProBoroArg is absorbed by a predominantly active transport mechanism (Saitoh, H. and Aungst, B. J.,  Pharm. Res,  1999, 16, 1786-1789). 
     The peptide boronic acid formed by such cleavage of TRI 50b (the acid is designated TRI 50c) Is relatively insoluble in water, especially at acidic or neutral pH, and tends to be poorly absorbed in the stomach and duodenum. The acid has the structure Cbz-Phe-Pro-BoroMpg-OH. 
     Whereas the peptide boronic add Cbz-Phe-Pro-BoroMpg-OH is partly ionised under duodenal conditions and, to that extent, unfavoured for passive transport, esters of the acid are designed for a high rate of passive (thus consistent) transport. The tripeptide sequence Phe-Pro-Mpg has a non-basic Pi side chain (specifically, methoxypropyl), such that the tripeptide consists of three non-polar amino acids. The esters of the peptide boronic acid are non-lonisable and the ester-forming species further impart lipophilic properties, so encouraging a high rate of passive transport. 
     Computational techniques have confirmed that TRI 50b and other diol esters of Cbz-Phe-Pro-BoroMpg-OH can be predicted to have good bioavailability. Thus, polar surface area (PSAd) is a parameter predictive of bioavailability and PSAd values of greater than 60A correlate well with passive transcellular transport and with bioavailability of known drugs (Kelder,  J. Pharm. Res.,  1999, 16, 1514-1519). Measurements for diol esters of the above peptide boronic acid, including the p1 nacoi ester TRI 50b, show that the did esters have PSAd values well above 60 Å, predictive of passive transport and good bioavailability as shown in Table 1: 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 PSAd values of selected diol esters of Cbz-Phe-Pro-BoroMpg-OH 
               
            
           
           
               
               
               
            
               
                   
                 Diol 
                 PSAd 
               
               
                   
                   
               
               
                   
                 Pinacol 
                 98.74 
               
               
                   
                 Pinanediol 
                 90.64 
               
               
                   
                   
               
            
           
         
       
     
     The corresponding monohydroxy alcohol (e.g. alkanol) esters were considered too unstable, spontaneously cleaving to liberate the acid in-vitro. Esters of diols such as pinanediol and pinacol have enhanced kinetic stability over esters of monohydroxy alcohols, in that after partial hydrolysis to the mono-ester derivative they will tend to reassociate by a facile intra-molecular reaction. 
     BRIEF SUMMARY OF THE DISCLOSURE 
     To counterbalance these highly desirable features of TRI 50b, it has been discovered that TRI 50b tends to hydrolyse. Thus in the acid conditions of an HPLC assay, TRI 50b is converted to the acid form with a short half life, which implies potential intraduodenal hydrolysis in the duodenum and elsewhere in the gastro-intestinal tract into ionic species which would resist passive transport and, if anything, be absorbed by active transport, indicative at best of variable bioavailability. 
     The instability of TRI 50b to hydrolysis also presents potential disadvantages In preparation of the compound and its formulation, as well as in the storage of pharmaceutical formulations containing it. 
     Another challenging difficulty which has been posed by TRI 50b is that the data show significant variation in bioavailability between subjects. Such variability can make a drug candidate unacceptable and it would therefore be desirable to reduce the observed variability. 
     An ideal solution to the instability of TRI 50b would be development of a diol ester more stable to hydrolysis, as such a diol ester like TRI 50b can be predicted to be oxidation resistant as compared with TRI 50c. In this regard, it is known that ring size can affect boronate stability and glycolato boron has been shown to have enhanced aqueous stability compared to pinacol (D. S. Matteson, Stereodirected Synthesis with Organoboranes, Springer-Verlag, 1995, ch.1). Similarly, the pinanediol ester is more stable than the pinacol; this is believed to be because the pinanediol group is highly sterically hindered and disfavours nucleophilic attack on the boron. In fact transesterification from pinacol to pinanediol has been reported (Brosz, C S,  Tet. Assym,  8:1435-1440, 1997) whereas the reverse process is unfavourable. The pinanediol ester however is considered too slow to cleave in plasma and there remains a need to provide an improved diol ester. 
     Another solution to the instability of TRI 50b would be to administer in its place TRI 50c. However, TRI 50c data suggest that TRI 50c too suffers from variability in bioavailability, 
     TRI 50c suffers further from instability, in that there is a problematic tendency for the boropeptide moiety itself to degrade via de-boronation (carbon-boron bond cleavage), by a pathway previously considered to be oxidative as taught by the literature (e.g. Wu et al, discussed above). The level of degradation can be remarkably high. 
     The properties discussed above of TRI 50b and TRI 50c will not be restricted to such compounds but will be shared by other boropeptide esters and acids, even if the properties of such other boropeptides differ quantitatively. 
     The present disclosure is predicated on, amongst other things, the finding that certain organoboronic acid products are indicated to be of enhanced stability. 
     The benefits of the present disclosure include a solution to the problem of boronate diol ester and especially TRI 50b instability, that is to say the presently disclosed products provide inter alia pharmacologically active compounds which are more stable than TRI 50b and other comparable esters in the sense of stability to hydrolysis. The disclosure further includes a solution to the problem of organoboronic acid instability, that is to say the presently disclosed products provide inter alia pharmacologically active compounds which are more stable to deboronation than TRI 50c. The stability provided within the framework of the disclosure is not absolute but is improved relative to the comparator compounds. The benefits offered by the disclosure further include the provision of unexpected products which have usefulness in oral formulations. 
     There is disclosed an amino boronic acid derivative which avoids the disadvantages of pinacol esters. The disclosure further includes a peptide boronic acid derivative which is indicated to be of enhanced stability. In particular, the disclosure includes amongst other subject a pharmaceutical formulation which comprises an amino boronic acid derivative and which can provide in the duodenum an ionic boropeptide species whilst avoiding the disadvantages of pinacol esters, and can provide bioavailability. Further included are boronic acid derivatives which are of relative stability to hydrolysis and deboronation and are useful in oral formulations for inhibiting thrombin. 
     The disclosure concerns a pharmaceutically acceptable base addition salt of certain organoboronic acid drugs, specifically hydrophobic boropeptides (e.g di- or tri-peptides), and more specifically thrombin inhibitors having a non-basic P1 group. As a class, such salts are not only contrary to the direction of the prior art but additionally have an improved level of stability which cannot be explained or predicted on the basis of known chemistry. 
     In one aspect, the disclosure relates to pharmaceutically acceptable base addition salts of boronic acids which have a neutral aminoboronic acid residue capable of binding to the thrombin S1 subsite linked through a peptide linkage to a hydrophobic moiety capable of binding to the thrombin S2 and S3 subsites. In a first embodiment, there is disclosed a pharmaceutically acceptable base addition salt of a boronic acid of, for example, formula (I): 
     
       
         
         
             
             
         
       
     
     wherein 
     Y comprises a hydrophobic moiety which, together with the aminoboronic acid residue 
     —NHCH(R 9 )—B(OH) 2 , has affinity for the substrate binding site of thrombin; and 
     R 9  is a straight chain alkyl group interrupted by one or more ether linkages (e.g. 1 or 2) and in which the total number of oxygen and carbon atoms is 3, 4, 5 or 6 (e.g. 5) or R 9  is —(CH 2 ) m —W where m is 2, 3, 4 or 5 (e.g. 4) and W is —OH or halogen (F, Cl, Br or I). R 9  is an alkoxyalkyl group in one subset of compounds, e.g. alkoxyalkyl containing 4 carbon atoms. 
     The disclosure comprises pharmaceutically acceptable base addition salts of hydrophobic boronic acid inhibitors of thrombin, and therefore includes such salts of peptide boronic acids which have a partition coefficient between 1-n-octanol and water expressed as log P of greater than 1.0 at physiological pH and 25° C. Some peptide boronic acids useful in the disclosure have a partition coefficient of at least 1.5. A class of hydrophobic peptide boronic acids useful in the disclosure has a partition coefficient of no more than 5. 
     Disclosed as certain examples are pharmaceutically acceptable base addition salts of hydrophobic boronic acid inhibitors of thrombin. Such inhibitors may contain hydrophobic amino acids, and this class of amino acids includes those whose side chain is hydrocarbyl, hydrocarbyl containing an in-chain oxygen and/or linked to the remainder of the molecule by an in-chain oxygen or heteroaryl, or any of the aforesaid groups when substituted by hydroxy, halogen or trifluoromethyl. Representative hydrophobic side chains include alkyl, alkoxyalkyl, either of the aforesaid when substituted by at least one aryl or heteroaryl, aryl, heteroaryl, aryl substituted by at least one alkyl and heteroaryl substituted by at least one alkyl. Proline and other imino acids which are ring-substituted by nothing or by one of the moieties listed in the previous sentence are also hydrophobic. 
     Some hydrophobic side chains contain from 1 to 20 carbon atoms, e.g. non-cyclic moieties having 1, 2, 3 or 4 carbon atoms. Side chains comprising a cyclic group typically but not necessarily contain from 5 to 13 ring members and in many cases are phenyl or alkyl substituted by one or two phenyls. 
     Hydrophobic non-peptides are typically based on moieties which may form a side chain of a hydrophobic amino acid, as described above. 
     Hydrophobic compounds may contain, for example, one amino group and/or one acid group (e.g. —COOH, —B(OH) 2 ). Generally, they do not contain multiple polar groups of any one type. 
     One class of hydrophobic organoboronic acids have a partition coefficient between 1-n-octanol and water expressed as log P of greater than 1 at physiological pH and 25′C. For example, TRI 50c has a partition coefficient of approximately 2. 
     Some sub-classes of hydrophobic organoboronic acids are those described by Formulae (I) and (III)) below, under the heading “Detailed Description of Several Examples”. 
     Also disclosed as another embodiment is a pharmaceutically acceptable base addition salt of a 
     
       
         
         
             
             
         
       
     
     peptide boronic add of formula (II):
 
where:
 
X is a moiety bonded to the N-terminal amino group and may be H to form NH 2 . The identity of X is not critical but may be a particular X moiety described above. In one example there may be mentioned benzyloxycarbonyl.
 
aa 1  is an amino acid having a hydrocarbyl side chain containing no more than 20 carbon atoms (e.g. up to 15 and optionally up to 13 C atoms) and comprising at least one cyclic group having up to 13 carbon atoms. In certain examples, the cyclic group(s) of aa 1  have/has 5 or 6 ring members. For instance, the cyclic group(s) of aa 1  may be aryl groups, particularly phenyl. Typically, there are one or two cyclic groups in the aa 1  side chain. Certain side chains comprise, or consist of, methyl substituted by one or two 5- or 6-membered rings.
 
     More particularly, aa 1  is Phe, Dpa or a wholly or partially hydrogenated analogue thereof. The wholly hydrogenated analogues are Cha and The disclosure therefore includes medicaments comprising salts, e.g. metal salts, of organoboronic acids which are thrombin inhibitors, particularly selective thrombin inhibitors, having a neutral P1 (Si-binding) moiety. For more information about moieties which bind to the S3, S2 and Si sites of thrombin, see for example Tapparelli C et al,  Trends Pharmacol. Sci.  14: 366-376, 1993; Sanderson P et al,  Current Medicinal Chemistry,  5: 289-304, 1998; Rewinkel 3 et al,  Current Pharmaceutical Design,  5:1043-1075, 1999; and Coburn C  Exp. Opin. Ther. Patents  11(5): 721-738, 2001. The thrombin inhibitory salts of the disclosure are not limited to those having S3, S2 and S1 affinity groups described in the publications listed In the preceding sentence. 
     Dcha. 
     aa 2  is an imino acid having from 4 to 6 ring members. Alternatively, aa 2  is Gly N-substituted by a C 3 -C 13  hydrocarbyl group, e.g. a C 3 -C 8  hydrocarbyl group comprising a C 3 -C 6  hydrocarbyl ring; the hydrocarbyl group may be saturated, for example exemplary N-substituents are cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. As a hydrocarbyl group containing one or more unsaturated bonds may be mentioned phenyl and methyl or ethyl substituted by phenyl, e.g. 2-phenylethyl, as well as β, β-dialkylphenylethyl. 
     There is a debate in the literature as to whether boronates in aqueous solution form the ‘trigonal’ B(OH) 2  or ‘tetrahedral’ B(OH) 3   −  boron species, but NMR evidence seems to indicate that at a pH below the first pKa of the boronic acid the main boron species is the neutral B(OH) 2 . In the duodenum the pH is likely to be between 6 and 7, so the trigonal species is likely to be predominant here. In any event, the symbol —B(OH) 2  includes tetrahedral as well as trigonal boron species, and throughout this specification symbols indicating trigonal boron species embrace also tetrahedral species. The symbol further may further include boronic groups in anhydride form. 
     The salts may be in the form of solvates, particularly hydrates. 
     The salts may comprise, or consist essentially of, acid salts in which the boronic acid is singly deprotonated. The disclosure therefore includes products having a metal/boronate stoichiometry consistent with the boronate groups in the product predominantly (more than 50 mol %) carrying a single negative charge. 
     Oral formulations of the salts are also provided herein. In particular, there are provided oral formulations comprising the salts in the solid phase, for example particulate salts formulated as compressed tablets or as capsules. 
     According to a further aspect of the present disclosure, there is provided a method of treatment of a condition where anti-thrombotic activity is required which method comprises oral administration of a therapeutically effective amount of a salt of a boronic acid of formula (I) to a person suffering from, or at risk of suffering from, such a condition. 
     As described further hereinafter, there are provided also haemodialysis solutions comprising a salt of the disclosure. 
     The salts described herein include products obtainable by (having the characteristics of a product obtained by) reaction of the boronic acid with a strong base and the term “salt” herein is to be understood accordingly. The term “salt” In relation to the disclosed products, therefore, does not necessarily imply that the products contain discrete cations and anions and is to be understood as embracing products which are obtainable using a reaction of a boronic acid and a base. The disclosure embraces products which, to a greater or lesser extent, are in the form of a coordination compound. The disclosure thus provides also products obtainable by (having the characteristics of a product obtained by) reaction of a boronic acid (I) with a strong base a well as the therapeutic, including prophylactic, use of such products. 
     The present disclosure is not limited as to the method of preparation of the salts, provided that they contain a boronate species derived from boronic acid (I) and a counter-ion. Such boronate species may be boronate anions in any equilibrium form thereof. The term “equilibrium form” refers to differing forms of the same compounds which may be represented in an equilibrium equation (e.g. boronic acid in equilibrium with a boronic anhydride and in equilibrium with different boronate ions). Boronates in the solid phase may form anhydrides and the disclosed boronate salts when in the solid phase may comprise boronate anhydrides, as a boronic equilibrium species. It is not required that the salts be prepared by reaction of a base containing the counter-ion and the boronic acid (I). Further, the disclosure includes salt products which might be regarded as indirectly prepared by such an acid/base reaction as well as salts obtainable by (having the characteristics of products obtained by) such indirect preparation. As examples of possibly indirect preparation may be mentioned processes in which, after initial recovery of the salt, it is purified and/or treated to modify its physicochemical properties, for example to modify solid form or hydrate form, or both. 
     In some embodiments, the cations of the salts are monovalent. 
     In some embodiments the salts comprise anhydride species; in others they are essentially free of anhydride species. 
     The salts may be in isolated form. The salts may have a purity, e.g. as determined by the method of Example 28, of at least about 90%, e.g. of greater than or equal to about 95%. In the case of pharmaceutical formulations, such salt forms may be combined with pharmaceutically acceptable diluents, excipients or carriers. 
     The disclosure includes a method for preparing the salts from the corresponding boronic acid as an intermediate, as well as the intermediate boronic acid of Formula (I) and a method for preparing it. 
     Further aspects and embodiments of the disclosure are set forth in the following description and claims. 
     Throughout the description and claims of this specification, the words “comprise” and “contain” and variations of the words, for example “comprising” and “comprises”, mean “including but not limited to”, and are not intended to (and do not) exclude other moieties, additives, components, integers or steps. 
     This patent application contains data indicating that the stability (resistance to deboronation) of organoboronic acids may be increased by providing them in the form of salts, e.g. metal salts. In single experiments, the ammonium salt of TRI 50c appeared to decompose on drying to yield ammonia, whilst the choline salt demonstrated rapid decomposition to a deboronated impurity. Although experiments have not been conducted to reproduce these unrepeated observations, there is provided a sub-class in which the ammonium and choline salts are excluded. The salt may be an acid salt. In any event, this stabilisation technique forms part of the disclosure and is applicable, inter alia, to organoboronic acids described under the heading “BACKGROUND” and to organoboronic adds described in publications mentioned under that heading. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a plot referred to in Example 32, showing oral phase clearance and kinetics following p.o. dosing with TRI S0b or TRI 50c. 
         FIG. 2  is a second plot referred to in Example 32, showing oral phase clearance and kinetics following intraduodenal dosing with TRI 50b or TRI 50c. 
     
    
    
     DETAILED DESCRIPTION OF SEVERAL EXAMPLES 
     Glossary 
     The following terms and abbreviations are used in this specification: 
     The expression “acid salt” as applied to a salt of a boronic acid refers to salts of which a single —OH group of the trigonally-represented acid group —B(OH) 2  is deprotonated. Thus salts wherein the boronate group carries a single negative charge and may be represented as —B(OH)(O − ) or as [—B(OH) 3 ] −  are acid salts. The expression encompasses salts of a cation having a valency n wherein the molar ratio of boronic acid to cation is approximately n to 1. In practical terms, the observed stoichiometry is unlikely to be exactly n:1 but will be consistent with a notional n:1 stoichiometry. For example, the observed mass of the cation might vary from the calculated mass for a n:1 stoichiometry by no more than about 10%, e.g. no more than about 7.5%; in some cases an observed mass of a cation might vary from the calculated mass by no more than about 1%. Calculated masses are suitably based on the trigonal form of the boronate. (At an atomic level, a salt stoichiometrically consistent with being an acid salt might contain boronates in a mix of protonation states, whose average approximates to single deprotonation and such “mixed” salts are included in the term “acid salt”). Examples of acid salts are monosodium salts and hemizinc salts. 
     α-Aminoboronic acid or Boro(aa) refers to an amino acid in which the CO 2  group has been replaced by BO 2 . 
     The term “amino-group protecting moiety” refers to any group used to derivatise an amino group, especially an N-terminal amino group of a peptide or amino acid. Such groups include, without limitation, alkyl, acyl, alkoxycarbonyl, aminocarbonyl, and sulfonyl moieties. However, the term “amino-group protecting moiety” is not intended to be limited to those particular protecting groups that are commonly employed in organic synthesis, nor is it intended to be limited to groups that are readily cleavable. 
     The phrase “pharmaceutically acceptable” is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings or animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio. 
     The expression “thrombin inhibitor” refers to a product which, within the scope of sound pharmacological judgement, is potentially or actually pharmaceutically useful as an inhibitor of thrombin, and includes reference to substance which comprises a pharmaceutically active species and is described, promoted or authorised as a thrombin inhibitor. Such thrombin inhibitors may be selective, that is they are regarded, within the scope of sound pharmacological judgement, as selective towards thrombin in contrast to other proteases; the term “selective thrombin inhibitor” includes reference to substance which comprises a pharmaceutically active species and is described, promoted or authorised as a selective thrombin inhibitor. 
     The term “heteroaryl” refers to a ring system which has at least one (e.g. 1, 2 or 3) in-ring heteroatoms and has a conjugated in-ring double bond system. The term “heteroatom” includes oxygen, sulfur and nitrogen, of which sulfur is sometimes less preferred. 
     “Natural amino acid” means an L-amino acid (or residue thereof) selected from the following group of neutral (hydrophobic or polar), positively charged and negatively charged amino acids: 
     Hydrophobic Amino Acids 
     A=Ala=alanine 
     V=Val=valine 
     I=Ile=isoleucine 
     L=Leu=leucine 
     M=Met=methionine 
     F=Phe=phenylalanine 
     P=Pro=proline 
     W=Trp=tryptophan 
     Polar (Neutral or Uncharged) Amino Acids 
     N=Asn=asparagine 
     C=Cys=cysteine 
     Q=Gin=glutamine 
     G=Gly=glycine 
     S=Ser=serine 
     T=Thr=threonine Y=Tyr=tyrosine 
     Positively Charged (Basic) Amino Acids 
     R=Arg=arginine 
     H=His=histidine K=Lys=lysine 
     Negatively Charged Amino Acids 
     D=Asp=aspartic acid 
     E=Glu=glutamic acid. 
     ACN=acetonitrile
 
Amino acid=α-amino acid
 
Base addition salt=a salt which is prepared from addition of an inorganic base or an organic base to a free acid (in this case the boronic acid).
 
Cbz=benzyloxycarbonyl
 
Cha=cyclohexylalanine (a hydrophobic unnatural amino acid)
 
Charged (as applied to drugs or fragments of drug molecules, e.g. amino acid residues)=carrying a charge at physiological pH, as in the case of an amino, amidino or carboxy group
 
Dcha=dicyclohexylalanine (a hydrophobic unnatural amino acid)
 
Dpa=diphenylalanine (a hydrophobic unnatural amino acid)
 
Drug=a pharmaceutically useful substance, whether the active in vivo principle or a prodrug
 
i.v.=intravenous
 
Mpg=3-methoxypropylglycine (a hydrophobic unnatural amino acid)
 
Multivalent=valency of at least two, for example two or three
 
Neutral (as applied to drugs or fragments of drug molecules, e.g. amino acid residues)=uncharged=not carrying a charge at physiological pH
 
Pinac=Pinacol=2,3-dimethyl-2,3-butanediol
 
Pinanediol=2,3-pinanediol=2,6,6-trimethylbicyclo[3.1.1]heptane-2,3-diol
 
Pip=pipecolinic acid
 
p.o.=per os= by way of the mouth (thus an oral formulation is administered p.o.)
 
s.c.=subcutaneous
 
Strong base=a base having a sufficiently high pKb to react with a boronic acid. Suitably such bases have a pKb of 7 or more, e.g. 7.5 or more, for example about 8 or more
 
THE=tetrahydrofuran
 
Thr=thrombin
 
     The Compounds 
     The products of the disclosure comprise salts of boronic acids which have a neutral aminoboronic acid residue capable of binding to the thrombin S1 subsite linked through a peptide linkage to a hydrophobic moiety capable of binding to the thrombin S2 and S3 subsites. The disclosure includes salts of acids of formula (I): 
     
       
         
         
             
             
         
       
     
     wherein 
     Y comprises a hydrophobic moiety which, together with the aminoboronic acid residue —NHCH(R 9 )—B(OH) 2 , has affinity for the substrate binding site of thrombin; and 
     R 9  is a straight chain alkyl group interrupted by one or more ether linkages and in which the total number of oxygen and carbon atoms is 3, 4, 5 or 6 (e.g. 5) or R 9  is —(CH 2 )m-W where m is From 2, 3, 4 or 5 (e.g. 4) and W is —OH or halogen (F, Cl, Br or I). As examples of straight chain alkyl interrupted by one or more ether linkages (-0-) may be mentioned alkoxyalkyl (one interruption) and alkoxyalkoxyalkyl (two interruptions). R 9  is an alkoxyalkyl group In one subset of compounds, e.g. alkoxyalkyl containing 4 carbon atoms. 
     Typically, YCO— comprises an amino acid residue (whether natural or unnatural) which binds to the S2 subsite of thrombin, the amino acid residue being N-terminally linked to a moiety which binds the S3 subsite of thrombin. 
     In one class of Formula (I) acids, YCO— is an optionally N-terminally protected dipeptide residue which binds to the S3 and S2 binding sites of thrombin and the peptide linkages In the acid are optionally and independently N-substituted by a C 1 -C 13  hydrocarbyl group optionally containing in-chain and/or in-ring nitrogen, oxygen or sulfur and optionally substituted by a substituent selected from halo, hydroxy and trifluaromethyl. The N-terminal protecting group, when present, may be a group X as defined above (other than hydrogen). Normally, the add contains no N-substituted peptide linkages; where there is an N-substituted peptide linkage, the substituent is often 1 C to 6 C hydrocarbyl, e.g. saturated hydrocarbyl; the N-substituent comprises a ring in some embodiments, e.g. cycloalkyl, and may be cyclopentyl, for example. One class of acids has an N-terminal protecting group (e.g. an X group) and unsubstituted peptide linkages. 
     Where YCO— is a dipeptide residue (whether or not N-terminally protected), the S3-binding amino acid residue may be of R configuration and/or the S2-binding residue may of S configuration. The fragment —NHCH(R 9 )—B(OH) may of R configuration. The disclosure is not restricted to chiral centres of these conformations, however. 
     In one class of compounds, the side chain of P3 (S3-binding) amino acid and/or the P2 (S2-binding) amino acid is a moiety other than hydrogen selected from a group of formula A or B: 
       —(CO) a —(CH 2 ) b -D c -(CH 2 ) d   −E   (A) 
       —(CO) a —(CH 2 ) b -D c -C e (E 1 )(E 2 )(E 3 )  (B) 
     wherein 
     a is 0 or 1; 
     e is 1; 
     b and d are independently 0 or an integer such that (b+d) is from 0 to 4 or, as the case may be, (b+e) is from 1 to 4; 
     c is 0 or 1; 
     D is O or S; 
     E is H, C 1 -C 6  alkyl, or a saturated or unsaturated cyclic group which normally contains up to 14 members and particularly is a 5-6 membered ring (e.g. phenyl) or an 8-14 membered fused ring system (e.g. naphthyl), which alkyl or cyclic group is optionally substituted by up to 3 groups (e.g. 1 group) independently selected from C 1 -C 6  trialkylsilyl, —CN, —R 13 ,  − R 1-20 R 13 , —R 12 COR 13 , —R 12 CO 2 R 13  and —R 12 O 2 CR 13 , wherein R 12  is —(CH 2 )f and R 13  is —(CH 2 )gH or by a moiety whose non-hydrogen atoms consist of carbon atoms and in-ring heteroatoms and number from 5 to 14 and which contains a ring system (e.g. an aryl group) and optionally an alkyl and/or alkylene group, wherein f and g are each independently from 0 to 10, g particularly being at least 1 (although —OH may also be mentioned as a substituent), provided that (f+g) does not exceed 10, more particularly does not exceed 6 and most particularly is 1, 2, 3 or 4, and provided that there is only a single substituent if the substituent is a said moiety containing a ring system, or E is C 1 -C 6  trialkylsilyl; and E 1 , E 2  and E 3  are each independently selected from —R 15  and -J-R 15 , where J is a 5-6 membered ring and R 15  is selected from C 1 -C 6  trialkylsilyl, —CN, —R 13 , —R 1-20 R 13 , —R 12 COR 13 , —R 12 CO 2 R 13 , —R 12 O 2 CR 13 , and one or two halogens (e.g. in the latter case to form a -J-R 15  moiety which is dichlorophenyl), where R 12  and R 13  are, respectively, an R 12  moiety and an R 13  moiety as defined above (in some acids where E 1 , E 2  and E 3  contain an R 13  group, g is 0 or 1); 
     in which moiety of Formula (A) or (B) any ring is carbocyclic or aromatic, or both, and any one or more hydrogen atoms bonded to a carbon atom is optionally replaced by halogen, especially F. 
     In certain examples, a is 0. If a is 1, c may be 0. In particular examples, (a+b+c+d) and (a+b+c+e) are no more than 4 and are more especially 1, 2 or 3. (a+b+c+d) may be 0. 
     Exemplary groups for E, E 1 , E 2  and E 3  include aromatic rings such as phenyl, naphthyl, pyridyl, quinolinyl and furanyl, for example; non-aromatic unsaturated rings, for example cyclohexenyl; saturated rings such as cyclohexyl, for example. E may be a fused ring system containing both aromatic and non-aromatic rings, for example fluorenyl. One class of E, E 1 , E 2  and E 3  groups are aromatic (including heteroaromatic) rings, especially 6-membered aromatic rings. In some compounds, E 1  is H whilst E 2  and E 3  are not H; in those compounds, examples of E 2  and E 3  groups are phenyl (substituted or unsubstituted) and C 1 -C 4  alkyl, e.g. methyl. 
     In one class of embodiments, E contains a substituent which is C 1 -C 6  alkyl, (C 1 -C 5  alkyl)carbonyl, carboxy C 1 -C 5  alkyl, aryl (including heteroaryl), especially 5-membered or preferably 6-membered aryl (e.g. phenyl or pyridyl), or arylalkyl (e.g. arylmethyl or arylethyl where aryl may be heterocyclic and is preferably 6-membered). 
     In another class of embodiments, E contains a substituent which is OR 13 , wherein R 13  can be a 6-membered ring, which may be aromatic (e.g. phenyl) or is alkyl (e.g. methyl or ethyl) substituted by such a 6-membered ring. 
     A class of moieties of formula A or B are those in which E is a 6-membered aromatic ring optionally substituted, particularly at the 2-position or 4-position, by —R 13  or —OR 13 . 
     The disclosure includes salts in which the P3 and/or P2 side chain comprises a cyclic group in which 1 or 2 hydrogens have been replaced by halogen, e.g. F or Cl. 
     The disclosure includes a class of salts in which the side chains of formula (A) or (B) are of the following formulae (C), (D) or (E): 
     
       
         
         
             
             
         
       
     
     wherein q is from 0 to 5, e.g. is 0, 1 or 2, and each T is independently hydrogen, halogen (e.g. F or CI), —SiMe 3 , —R 13 , —OR 13 , —COR 13 , —CO 2 R 13  or —O 2 CR 13 . In some embodiments of structures (D) and (E), T is at the 4-position of the phenyl group(s) and is —R 13 , —OR 13 , —COR 13 , —CO 2 R 13  or —O 2 CR 13 , and R 13  is C 1 -C 10  alkyl and more particularly C 1 -C 6  alkyl. In one sub-class, T is —R 13  or —OR 13 , for example in which f and g are each independently 0, 1, 2 or 3; in some side chains groups of this sub-class, T is —R 12 OR 13  and R 13  is H. 
     In one class of the moieties, the side chain is of formula (C) and each T is independently R 13  or OR 13  and R 13  is C 1 -C 4  alkyl. In some of these compounds, R 13  is branched alkyl and in others it is straight chain. In some moieties, the number of carbon atoms is from 1 to 4. 
     In many dipeptide fragments YCO— (which dipeptides may be N-terminally protected or not), the P3 amino acid has a side chain of formula (A) or (B) as described above and the P2 residue is of an imino acid. 
     The disclosure therefore includes medicaments comprising salts, e.g. metal salts, of organoboronic acids which are thrombin inhibitors, particularly selective thrombin inhibitors, having a neutral P1 (S1-binding) moiety. For more information about moieties which bind to the S3, S2 and S1 sites of thrombin, see for example Tapparelli C et al,  Trends Pharmacol. Sci.  14: 366-376, 1993; Sanderson P et al,  Current Medicinal Chemistry,  5: 289-304, 1998; Rewinkel J et al,  Current Pharmaceutical Design,  5:1043-1075, 1999; and Coburn C  Exp. Opin. Ther. Patents  11(5): 721-738, 2001. The thrombin inhibitory salts of the disclosure are not limited to those having S3, S2 and S1 affinity groups described in the publications listed in the preceding sentence. 
     The boronic acids may have a Ki for thrombin of about 100 nM or less, e.g. about 20 nM or less. A subset of the Formula (I) acids comprises the acids of Formula (III): 
     
       
         
         
             
             
         
       
     
     X is a moiety bonded to the N-terminal amino group and may be H to form NH 2 . The identity of X is not critical but may be a particular X moiety described above. In one example there may be mentioned benzyloxycarbonyl. 
     In certain examples X is R 6 —(CH 2 )p-C(O)—, R 6 —(CH 2 )p S(O) 2 —, R 6 —(CH 2 )p NH—C(O)— or R 6 —(CH 2 )p-O—C(O)— wherein p is 0, 1, 2, 3, 4, 5 or 6 (of which 0 and 1 are preferred) and R 6  is H or a 5 to 13-membered cyclic group optionally substituted by 1, 2 or 3 substituents selected from halogen, amino, nitro, hydroxy, a C 5 -C 6  cyclic group, C 1 -C 4  alkyl and C 1 -C 4  alkyl containing, and/or linked to the 5 to 13-membered cyclic group through, an in-chain 0, the aforesaid alkyl groups optionally being substituted by a substituent selected from halogen, amino, nitro, hydroxy and a C 5 -C 6  cyclic group. More particularly X is R 6 —(CH 2 ) p  C(O)— or R 6 —(CH 2 )p O—C(O)— and p is 0 or 1. Said 5 to 13-membered cyclic group is often aromatic or heteroaromatic, for example is a 6-membered aromatic or heteroaromatic group. In many cases, the group is not substituted. 
     Exemplary X groups are (2-pyrazine) carbonyl, (2-pyrazine) sulfonyl and particularly benzyloxycarbonyl. 
     aa 1  is an amino acid residue having a hydrocarbyl side chain containing no more than 20 carbon atoms (e.g. up to 15 and optionally up to 13 C atoms) and comprising at least one cyclic group having up to 13 carbon atoms. In certain examples, the cyclic group(s) of aa 1  have/has 5 or 6 ring members. For instance, the cyclic group(s) of aa 1  may be aryl groups, particularly phenyl. Typically, there are one or two cyclic groups in the aa 1  side chain. Certain side chains comprise, or consist of, methyl substituted by one or two 5- or 6-membered rings. 
     More particularly, aa 1  is Phe, Dpa or a wholly or partially hydrogenated analogue thereof. The wholly hydrogenated analogues are Cha and Dcha. aa 2  is an imino acid residue having from 4 to 6 ring members. 
     An exemplary class of products comprises those in which aa 2  is a residue of an imino acid of formula (IV) 
     
       
         
         
             
             
         
       
     
     where R 11  is —CH 2 —, CH 2 —CH 2 —, —S—CH 2 — or —CH 2 —CH 2 —CH 2 —, which group when the ring is 5 or 6-membered is optionally substituted at one or more —CH 2 — groups by from 1 to 3 C 1 -C 3  alkyl groups, for example to form the R 11  group —S—C(CH 3 ) 2 —. Of these imino acids, azetidine-2-carboxylic acid, especially (s)-azetidine-2-carboxylic acid, and more particularly proline are illustrative. 
     It will be appreciated from the above that a very preferred class of products consists of those in which aa 1 -aa 2  is Phe-Pro. In another preferred class, aa 1 -aa 2  is Dpa-Pro. In other products, aa 1 aa 2  is Cha-Pro or Dcha-Pro. Of course, also included are corresponding product classes in which Pro is replaced by (s)-azetidine-2-carboxylic acid. 
     R 9  is as defined previously and may be a moiety R 1  of the formula —(CH 2 )s Z. Integer s is 2, 3 or 4 and W is —OH, —OMe, —OEt or halogen (F, Cl, I or, preferably, Br). Particularly illustrative Z groups are —OMe and —OEt, especially —OMe. In certain examples s is 3 for all Z groups and, indeed, for all compounds of the disclosure. Particular R 1  groups are 2-bromoethyl, 2-chloroethyl, 2-methoxyethyl, 4-bromobutyl, 4-chlorobutyl, 4-methoxybutyl and, especially, 3-bromopropyl, 3-chloropropyl and 3-methoxypropyl. Most preferably, R 1  is 3-methoxypropyl. 2-Ethoxyethyl is another preferred R 1  group. 
     Accordingly, a very preferred class of salts consists of those of acids of the formula X-Phe-Pro-Mpg-B(OH) 2 , especially Cbz-Phe-Pro-Mpg-B(OH) 2 ; also preferred are analogues of these compounds in which Mpg is replaced by a residue with another of the particularly preferred R 1  groups and/or Phe is replaced by Dpa or another aa 1  residue. 
     The aa 1  moiety of the salt is preferably of R configuration. The aa 2  moiety is preferably of (S)-configuration. Particularly preferred salts have aa 1  of (R)-configuration and aa 2  of (S)-configuration. The chiral centre —NH—CH(R 1 )—B— is preferably of (R)-configuration. It is considered that commercial formulations will have the chiral centres in (R,S,R) arrangement, as for example in the case of salts of Cbz-Phe-Pro-BoroMpg-OH: 
     
       
         
         
             
             
         
       
     
     The disclosure includes salts of Cbz-(R)-Phe-(S)-Pro-(R)-boroMpg-OH (and of other compounds of the formula X—(R)-Phe-(S)-Pro-(R)-boroMpg-OH) which are at least 90% pure, e.g. at least 95% pure. 
     In broad terms, the salts described herein may be considered to correspond to reaction products of an organoboronic acid as described above with a strong base, e,g. a basic metal compound; the salts are however not limited to products resulting from such a reaction and may be obtained by alternative routes. 
     The salts are therefore obtainable by contacting an acid of formula (I) with a strong base. The disclosure thus contemplates products (compositions of matter) having the characteristics of a reaction product of an acid of formula (I) and a strong base. The base is pharmaceutically acceptable. 
     As suitable salts may be mentioned salts of metals, e.g. of monovalent or divalent metals, and stronger organic bases, for example: 
     1. Alkali metal salts;
 
2. Divalent, e.g. alkaline earth metal, salts;
 
3. Group III metals;
 
4. Salts of strongly basic organic nitrogen-containing compounds, including:
         4A. Salts of guanidines and their analogues;   4B. Salts of strongly basic amine, examples of which include (i) aminosugars and (ii) other amines.       

     Of the above salts, particularly illustrative are alkali metals, especially Na and Li. Also illustrative are aminosugars. 
     Specific salts are of the acid boronate though in practice the acid salts may contain a very small proportion of the doubly deprotonated boronate. The term “acid boronate” refers to trigonal —B(OH) 2  groups in which one of the B—OH groups is deprotonated as well as to corresponding tetrahedral groups in equilibrium therewith. Acid boronates have a stoichiometry consistent with single deprotonation. 
     The disclosure includes therefore products (compositions of matter) which comprise salts which may be represented by formula (V): 
     
       
         
         
             
             
         
       
     
     where Y n+  is a pharmaceutically acceptable cation obtainable from a strong base, and aa 1 , aa 2 , X and R 1  are as defined above. Also included are products in which R 1  is replaced by another R 9  group. 
     One class of salts have a solubility of about 10 mM or more, e.g. of at least about 20 mM, when their solubility is determined as described in the examples at a dissolution of 25 mg/ml. More particularly yet they have a solubility of least 50 mM when their solubility is determined as described in the examples at a dissolution of 50 mg/ml. 
     The disclosure includes salts of boronic acids (I) having an observed stoichiometry consistent with the salt being of (being representable by) the formula “(boronate − )n cation n+ ”. One class of such salts are represented by the formula: 
       [Cbz-(R)-Phe-(S)-Pro-(R)-Mpg-B(OH)(O − )]M +   
     where M +  represents a monovalent cation, especially an alkali metal cation. It will be understood that the above representation is a notional representation of a product whose observed stoichiometry is unlikely to be literally and exactly 1:1. In any event, a particular salt is Cbz-(R)-Phe-(S)-Pro-(R)-Mpg-B(OH) 2  monosodium salt (TGN 255). In the above formula, the trigonally-represented boronate represents, as always, boronates which are trigonal, tetrahedral or mixed trigonal/tetrahedral. 
     Particularly exemplary are products which comprise: 
     (i) species selected from (a) acids of formula (VIII): X—(R.)-Phe-(S)-Pro-(R)-Mpg-B(OH) 2  where X is H or an amino-protecting group, especially Cbz, (b) boronate anions thereof, and (c) any equilibrium form of the aforegoing (e.g. an anhydride); and 
     (ii) ions having a valency n in combination with said species, the species and said ions having an observed stoichiometry consistent with a notional species:1 on stolchiometry of n:1. In one class of salts, n is 1. 
     Considering the counter-ions in turn: 
     1. Monovalent Metal, Especially Alkali Metal Salts 
     Suitable alkali metals include lithium, sodium and potassium. All of these are remarkably soluble. Lithium and sodium are illustrative because of their high solubility. The lithium and particularly sodium salts are of surprisingly high solubility in relation to potassium amongst others. Sodium is most used in many instances. Salts containing mixtures of alkali metals are contemplated by the disclosure. 
     The disclosure includes products comprising salts of the formula (VI) 
     
       
         
         
             
             
         
       
     
     where M +  is an alkali metal ion and aa 1 , aa 2 , X and R 1  are as defined above, as well as salts in which both hydroxy groups of the boronate group are in salt form (preferably with another identical M +  group) and mixtures of such salts. Included also are products wherein R 1  is replaced by another R 9  group.
 
2. Divalent, e.g. Alkaline Earth Metal (Group II Metal) Salts
 
     One example of a divalent metal is calcium. Another suitable divalent metal is magnesium. Also contemplated is zinc. The divalent metals are usually used in a boronic acid:metal ratio of substantially 2:1, in order to achieve the preferred monovalent boronate moiety. Salts containing mixtures of divalent metals, e.g. mixtures of alkaline earth metals, are also contemplated. 
     Further disclosed are products (compositions of matter) which comprise salts which may be represented by the formula (VII): 
     
       
         
         
             
             
         
       
     
     where M 2+  is a divalent metal cation, e.g. an alkaline earth metal or zinc cation, and aa 1 , aa 2′ , X and R 9  are as defined above, as well as salts in which both hydroxy groups of the boronate group are deprotonated and mixtures of such salts. As previously indicated, the boronate may comprise a tetrahedral species. 
     Thus the disclosure includes salts of the formula [Cbz-(R)-Phe-(S)-Pro-(R)-Mpg-B(OH)(O—)] 2 M 2+ , for example oral formulations containing [Cbz-(R)-Phe-(S)-Pro-(R)-Mpg-B(OH)(O — )] 2 Ca 2+ , suitably adapted for the salt to enter the duodenum without suffering gastric hydrolysis, e.g. by enteric coating. 
     3. Group III Metals 
     Suitable Group III metals include aluminium and gallium. Salts containing mixtures of Group III metals are also contemplated. 
     The disclosure includes products comprising salts of the formula (VIII): 
     
       
         
         
             
             
         
       
     
     where M 3+  is a Group III metal ion and aa 1 , aa 2′ , X and R 9  are as defined above, as well as salts in which both hydroxy groups of the boronate group are in salt form and mixtures of such salts. As previously indicated, the boronate may comprise a tetrahedral species. 
     4. Strongly Basic Organic Nitrogen-Containing Compounds 
     The disclosure includes products obtainable by (having the characteristics of a product obtained by) reaction of a peptide boronic acid as defined above and a strong organic base. Two illustrative dasses of organic base are described in sections 4A and 4B below. Particularly preferred are acid salts (in which one of the two boronic —OH groups is deprotonated). Most commonly, the salts contain a single type of organic counter-ion (disregarding trace contaminants) but the disclosure contemplates salts containing mixtures of organic counter-ions; in one sub-lass, the different counter-ions all fall within the section 4A family described below or, as the case may be, in the section 4B family below; in another subclass, the salts comprise a mixture of organic counter-ions which are not all from the same family (4A or 4B). 
     Suitable organic bases include those with a pKb of 7 or more, e.g. 7.5 or more, for example in the region of 8 or more. Bases which are less lipophilic [e.g. have at least one polar functional group (e.g. 1, 2 or 3 such groups) for example hydroxyl are favoured; thus aminosugars are one favoured class of base. 
     4A. Guanidines and their Analogues 
     The guanidino compound (guanidine) may in principle be any soluble and pharmaceutically acceptable compound having a guanidino or a substituted guanidino group, or a substituted or unsubstituted guanidine analogue. Suitable substituents include aryl (e.g. phenyl), alkyl or alkyl interrupted by an ether or thioether linkage and, in any event, typically contain from 1 to 6 and especially 1, 2, 3, or 4 carbon atoms, as in the case of methyl or ethyl. The guanidino group may have 1, 2, 3 or 4 substituent groups but more usually has 1 or 2 substituent groups, for instance on a terminal nitrogen. One class of guanidines is monoalkylated; another class is dialkylated. As guanidine analogues may be mentioned thioguanidines and 2-amino pyridines. Compounds having unsubstituted guanidino groups, for example guanidine and arginine, form one particular class. 
     Salts containing mixtures of guanidines are contemplated by the disclosure. 
     A particular guanidino compound is L-arginine or an L-arginine analogue, for example D-arginine, or the D- or, preferably, L-isomers of homoarginine or agmatine [(4-aminobutyl) guanidine]. Less preferred arginine analogues are NG-nitro-L-arginine methyl ester, for example, and constrained guanidine analogues, particularly 2-amino pyrimidines, for example 2,6-quinazolinediamines such as 5,6,7,8-tetrahydro-2,6-quinazolinediamine, for example. The guanidino compound may also be a peptide, for example a dipeptide, containing arginine; one such dipeptide is L-tyrosyl-L-arginine. 
     Some particular guanidino compounds are compounds of formula (VII): 
     
       
         
         
             
             
         
       
     
     where n is from 1 to 6 and for example at least 2, e.g. 3 or more, and In many instances no more than 5. Most particularly, n is 3, 4 or 5. R 2  is H or carboxylate or derivatised carboxylate, for example to form an ester (e.g. a C 1 -C 4  alkyl ester) or amide. R 3  is H, C 1 -C 4  alkyl or a residue of a natural or unnatural amino acid (e.g. tyrosine). The compounds of formula (IV) are usually of L-configuration. The compounds of formula (IV) are arginine (n=3; R 2 =carboxyl; R 3 =H) and arginine derivatives or analogues. 
     The disclosure includes products comprising salts of the formula (IX) 
     
       
         
         
             
             
         
       
     
     where aa 1 , aa 2 , X and R 1  are as defined previously and G +  is the protonated form of a pharmaceutically acceptable organic compound comprising a guanidino group or an analogue thereof, as well as salts in which both hydroxy groups of the boronate group are in salt form (preferably with another identical G +  group) and mixtures of such salts. Also included are products wherein R 1  is replaced by another R 9  group. 
     4B. Strongly Basic Amines 
     The disclosure includes products obtainable by (having the characteristics of a product obtained by) reaction of a peptide boronic acid as defined above and a strong organic base which is an amine. The amine may in principle be any soluble and pharmaceutically acceptable amine. 
     It is envisaged that a desirable class of amine includes those having polar functional groups in addition to a single amine group, as such compounds will be more hydrophilic and thus more soluble than others. In certain salts, the or each additional functional group is hydroxy. Some amines have 1, 2, 3, 4, 5 or 6 additional functional groups, especially hydroxy groups. In one illustrative class of amines the ratio of (amino plus hydroxy groups):carbon atoms is from 1:2 to 1:1, the latter ratio being particularly preferred. These amines with one or more additional polar functional groups may be a hydrocarbon, especially an alkane, substituted by the amino group and the additional polar group(s). The amino group may be substituted or unsubstituted and, excluding amino substituents, the polar base may contain, for example, up to 10 carbon atoms; usually there are no less than three such carbon atoms, e.g. 4, 5 or 6. Aminosugars are included in this category of polar bases. 
     The disclosure includes products comprising salts of the formula (X) 
     
       
         
         
             
             
         
       
     
     where aa 1 , aa 2 , X and R 1  are as defined previously and A +  is the protonated form of a pharmaceutically acceptable amine, as well as salts in which both hydroxy groups of the boronate group are in salt form (preferably with another identical A +  group) and mixtures of such salts. In one class of such products, A +  is the protonated form of an amine described in section 4B(i) below; in another class A +  is the protonated form of an amine described in 4B(ii) below. Also included are products in which R 1  is replaced by another R 9  group. 
     Two illustrative classes of amine base are described in sections 4B(i) and 4B(ii) below. Particularly preferred are acid salts (in which one of the two boronic —OH groups is deprotonated). Most commonly, the salts contain a single type of amine counter-ion (disregarding trace contaminants) but the disclosure contemplates salts containing mixtures of amine counter-ions; in one sub-class, the different counter-ions all fall within the sub-section 4B(i) family described below or, as the case may be, in the sub-section 4B(ii) family below; in another subclass, the salts comprise a mixture of organic counter-ions which are not all from the same family (4B(i) or 4B(ii)). 
     4B(i) Aminosugars 
     The identity of the aminosugar is not critical. Preferred aminosugars include ring-opened sugars, especially glucamines. Cyclic aminosugars are also envisaged as useful. One class of the aminosugars is N-unsubstituted and another, preferred, class is N-substituted by one or two N-substituents (e.g. one). Suitable substituents are hydrocarbyl groups, for example and without limitation containing from 1 to 12 carbon atoms; the substituents may comprise alkyl or aryl moieties or both. Exemplary substituents are C 1 , C 2 , C 3 , C 4 , C 5  C 6 , C 7  and C 8  alkyl groups, in particular methyl and ethyl, of which methyl is illustrative. Data indicate that aminosugars, especially N-methyl-D-glucamine, are of surprisingly high solubility. 
     A most preferred aminosugar is N-methyl-D-glucamine: 
     
       
         
         
             
             
         
       
     
     4B(ii) Other Amines 
     Other suitable amines include amino acids (whether naturally occurring or not) whose side chain is substituted by an amino group, especially lysine. 
     Some amines are compounds of formula (XI): 
     
       
         
         
             
             
         
       
     
     where n, R 2  and R 3  are as defined in relation to formula (IV). The compounds of formula (VI) are usually of L-configuration. The compounds of formula (VI) are lysine (n=4; R 2 =carboxyl; R 3 =H) and lysine derivatives or analogues. A most preferred amine is L-lysine. 
     Other suitable amines are nitrogen-containing heterocycles. At least usually, such heterocyclic compounds are alicyclic; one class of the heterocyclic compounds is N-substituted and another, preferred, class is N-unsubstituted. The heterocycles may contain 6 ring-forming atoms, as in the cases of piperidine, piperazine and morpholine. One class of amines includes N-containing heterocycles substituted by polar substituents, especially hydroxy, e.g. 1, 2 or 3 times, 
     The disclosure therefore includes amines other than aminosugars which have one or more (e.g. 1, 2, 3, 4, 5 or 6) polar substituents, especially hydroxy, in addition to one amine group. Such compounds may have a ratio of (amino plus hydroxy groups):carbon atoms of 1:2 to 1:1, the latter ratio being particularly preferred. 
     The disclosure includes mixed salts, i.e. salts containing a mixture of boropeptide moieties and/or counterions but single salts are preferred. 
     The salts in solid form may contain a solvent, e.g. water. There are included a class of products in which the salts are essentially anhydrous. Also included is a class in which the salts are hydrates. 
     Use of the Products of the Disclosure 
     The salts of the disclosure are thrombin inhibitors. They are therefore useful for inhibiting thrombin. There are therefore provided compounds which have potential for controlling haemostasis and especially for inhibiting coagulation, for example in the treatment or prevention of secondary events after myocardial infarction. The medical use of the compounds may be prophylactic (including to treat thrombosis as well as to prevent occurrence of thrombosis) as well as therapeutic (Including to prevent re-occurrence of thrombosis or secondary thrombotic events). 
     The salts may be employed when an anti-thrombogenic agent is needed. Further, it has been found that the salts, including those of boronic acids of Formula (III), are beneficial in that the class is useful for treating arterial thrombosis by therapy or prophylaxis. The disclosed salts are thus indicated in the treatment or prophylaxis of thrombosis and hypercoagulability in blood and tissues of animals including man. The term “thrombosis” includes inter alia atrophic thrombosis, arterial thrombosis, cardiac thrombosis, coronary thrombosis, creeping thrombosis, infective thrombosis, mesenteric thrombosis, placental thrombosis, propagating thrombosis, traumatic thrombosis and venous thrombosis. 
     It is known that hypercoagulability may lead to thromboembolic diseases. 
     Examples of venous thromboembolism which may be treated or prevented with compounds of the disclosure include obstruction of a vein, obstruction of a lung artery (pulmonary embolism), deep vein thrombosis, thrombosis associated with cancer and cancer chemotherapy, thrombosis inherited with thrombophilic diseases such as Protein C deficiency, Protein S deficiency, antithrombin III deficiency, and Factor V Leiden, and thrombosis resulting from acquired thrombophilic disorders such as systemic lupus erythematosus (inflammatory connective tissue disease). Also with regard to venous thromboembolism, compounds of the disclosure are useful for maintaining patency of indwelling catheters. 
     Examples of cardiogenic thromboembolism which may be treated or prevented with compounds of the disclosure include thromboembolic stroke (detached thrombus causing neurological affliction related to impaired cerebral blood supply), cardiogenic thromboembolism associated with atrial fibrillation (rapid, irregular twitching of upper heart chamber muscular fibrils), cardiogenic thromboembolism associated with prosthetic heart valves such as mechanical heart valves, and cardiogenic thromboembolism associated with heart disease. 
     Examples of conditions involving arterial thrombosis include unstable angina (severe constrictive pain in chest of coronary origin), myocardial infarction (heart muscle cell death resulting from insufficient blood supply), ischemic heart disease (local ischemia due to obstruction (such as by arterial narrowing) of blood supply), reocclusion during or after percutaneous transluminal coronary angioplasty, restenosis after percutaneous transluminal coronary angioplasty, occlusion of coronary artery bypass grafts, and occlusive cerebrovascular disease. Also with regard to arterio-venous (mixed) thrombosis, anti-thrombotic compounds of the disclosure are useful for maintaining patency in arteriovenous shunts. 
     Other conditions associated with hypercoagulability and thromboembolic diseases which may be mentioned inherited or acquired deficiencies in heparin cofactor II, circulating antiphospholipid antibodies (Lupus anticoagulant), homocysteinemia, heparin induced thrombocytopenia and defects in fibrinolysis. 
     Particular uses which may be mentioned include the therapeutic and/or prophylactic treatment of venous thrombosis and pulmonary embolism. Preferred indications envisaged for the products of the disclosure (notably the salts of TRI 50c) include:
         Prevention of venous thromboembolic events (e.g. deep vein thrombosis and/or pulmonary embolism). Examples include patients undergoing orthopedic surgery such as total hip replacement, total knee replacement, major hip or knee surgery; patients undergoing general surgery at high risk for thrombosis, such as abdominal or pelvic surgery for cancer; and in patients bedridden for more than 3 days and with acute cardiac failure, acute respiratory failure, infection.   Prevention of thrombosis in the haemodialysls circuit in patients, in patients with end stage renal disease.   Prevention of cardiovascular events (death, myocardial infarction, etc) in patients with end stage renal disease, whether or not requiring haemodialysis sessions.   Prevention of venous thrombo-embolic events in patients receiving chemotherapy through an indwelling catheter.   Prevention of thromboembolic events in patients undergoing lower limb arterial reconstructive procedures (bypass, endarterectomy, transluminal angioplasty, etc).   Treatment of venous thromboembolic events.   Prevention of cardiovascular events in acute coronary syndromes (e.g. unstable angina, non Q wave myocardial ischaemia/infarction), in combination with another cardiovascular agent, for example aspirin (acetylsalicylic acid; aspirin is a registered trade mark in Germany), thrombolytics (see below for examples), antiplatelet agents (see below for examples).   Treatment of patients with acute myocardial Infarction in combination with acetylsalicylic acid, thrombolytics (see below for examples).       

     The thrombin inhibitors of the disclosure are thus indicated both in the therapeutic and/or prophylactic treatment of all the aforesaid disorders. 
     In one method, the products of the disclosure are used for the treatment of patients by haemodialysis, by providing the product in the dialysis solution, as described in relation to other thrombin inhibitors in WO 00/41715. The disclosure therefore includes dialysing solutions and dialysing concentrates which comprise a product of the disclosure, as well as a method of treatment by dialysis of a patient in need of such treatment, which method comprises the use of a dialysing solution including a low molecular weight thrombin inhibitor. Also included is the use of an anti-thrombotic product of the disclosure for the manufacture of a medicament for the treatment by dialysis of a patient, in which the anti-thrombotic product of the disclosure is provided in the dialysing solution. 
     In another method, the products of the disclosure are used to combat undesirable cell proliferation, as described in relation to other thrombin inhibitors in WO 01/41796. The undesirable cell proliferation is typically undesirable hyperplastic cell proliferation, for example proliferation of smooth muscle cells, especially vascular smooth muscle cells. The products of the disclosure particularly find application in the treatment of intimal hyperplasia, one component of which is proliferation of smooth muscle cells. Restenosis can be considered to be due to neointimal hyperplasia; accordingly intimal hyperplasia in the context of the disclosure includes restenosis. 
     The products of the disclosure are also contemplated for the treatment of ischemic disorders, More particularly, they may be used in the treatment (whether therapeutic or prophylactic) of an ischemic disorder in a patient having, or at risk of, non-valvular atrial fibrillation (NVAF) as described In relation to other thrombin inhibitors in WO 02/36157. Ischemic disorders are conditions whose results include a restriction in blood flow to a part of the body. The term will be understood to include thrombosis and hypercoagulability in blood, tissues and/or organs. Particular uses that may be mentioned include the prevention and/or treatment of ischemic heart disease, myocardial infarction, systemic embolic events in e.g. the kidneys or spleen, and more particularly of cerebral ischemia, including cerebral thrombosis, cerebral embolism and/or cerebral ischemia associated with non-cerebral thrombosis or embolism (in other words the treatment (whether therapeutic or prophylactic) of thrombotic or ischemic stroke and of transient ischemic attack), particularly in patients with, or at risk of, NVAF. 
     The products of the disclosure are also contemplated for the treatment of rheumatic/arthritic disorders, as described in relation to other thrombin inhibitors in WO 03/007984. Thus, the products of the disclosure may be used in the treatment of chronic arthritis, rheumatoid arthritis, osteoarthritis or ankylosing spondylitis 
     Moreover, the products of the disclosure are expected to have utility in prophylaxis of re-occlusion (i.e. thrombosis) after thrombolysis, percutaneous trans-luminal angioplasty (PTA) and coronary bypass operations; the prevention of re-thrombosis after microsurgery and vascular surgery in general. Further indications include the therapeutic and/or prophylactic treatment of disseminated intravascular coagulation caused by bacteria, multiple trauma, intoxication or any other mechanism; anticoagulant treatment when blood is in contact with foreign surfaces In the body such as vascular grafts, vascular stents, vascular catheters, mechanical and biological prosthetic valves or any other medical device; and anticoagulant treatment when blood is in contact with medical devices outside the body such as during cardiovascular surgery using a heart-lung machine or in haemodialysis. 
     The products of the disclosure are further indicated in the treatment of conditions where there is an undesirable excess of thrombin without signs of hypercoagulability, for example in neurodegenerative diseases such as Alzheimer&#39;s disease. In addition to its effects on the coagulation process, thrombin is known to activate a large number of cells (such as neutrophils, fibroblasts, endothelial cells and smooth muscle cells). Therefore, the compounds of the disclosure may also be useful for the therapeutic and/or prophylactic treatment of idiopathic and adult respiratory distress syndrome, pulmonary fibrosis following treatment with radiation or chemotherapy, septic shock, septicaemia, inflammatory responses, which include, but are not limited to, edema, acute or chronic atherosclerosis such as coronary arterial disease, cerebral arterial disease, peripheral arterial disease, reperfusion damage, and restenosis after percutaneous transluminal angioplasty (PTA). 
     The salts may also be useful in the treatment of pancreatitis. 
     The salts described herein are further considered to be useful for inhibiting platelet procoagulant activity. The disclosure provides a method for inhibiting platelet pro-coagulant activity by administering a salt of a boronic acid described herein to a mammal at risk of, or suffering from, arterial thrombosis, particularly a human patient. Also provided is the use of such salts for the manufacture of medicaments for inhibiting platelet procoagulant activity. 
     The use of products of the disclosure as inhibitors of platelet pro-coagulant activity is predicated on the observation that the boronic acids described herein are indicated to be effective at inhibiting arterial thrombosis as well as venous thrombosis. 
     Indications involving arterial thrombosis include acute coronary syndromes (especially myocardial infarction and unstable angina), cerebrovascular thrombosis and peripheral arterial occlusion and arterial thrombosis occurring as a result of atrial fibrillation, valvular heart disease, arterio-venous shunts, indwelling catheters or coronary stents, Accordingly, in another aspect there is provided a method of treating a disease or condition selected from this group of indications, comprising administering to a mammal, especially a human patient, a salt of the disclosure. The disclosure includes products for use in an arterial environment, e.g. a coronary stent or other arterial implant, having a coating which comprises a salt according to the disclosure. 
     The salts of the disclosure may be used prophylactically to treat an individual believed to be at risk of suffering from arterial thrombosis or a condition or disease involving arterial thrombosis or therapeutically (including to prevent re-occurrence of thrombosis or secondary thrombotic events). 
     There is therefore included the use of selective thrombin inhibitors (organoboronic acid salts) described herein for treatment of the above disorders by prophylaxis or therapy as well as their use in pharmaceutical formulations and the manufacture of pharmaceutical formulations. 
     Administration and Pharmaceutical Formulations 
     The salts may be administered to a host, for example, in the case where the drug has antithrombogenic activity, to obtain an anti-thrombogenic effect. In the case of larger animals, such as humans, the compounds may be administered alone or in combination with pharmaceutically acceptable diluents, excipients or carriers. The term “pharmaceutically acceptable” includes acceptability for both human and veterinary purposes, of which acceptability for human pharmaceutical use is preferred. In the case of oral administration, the compounds are preferably administered in a form which prevents the salt of the disclosure from contact with the acidic gastric juice, such as enterically coated formulations, which thus prevent release of the salt of the disclosure until it reaches the duodenum. 
     The enteric coating is suitably made of carbohydrate polymers or polyvinyl polymers, for example. Examples of enteric coating materials include, but are not limited to, cellulose acetate phthalate, cellulose acetate succinate, cellulose hydrogen phthalate, cellulose acetate trimellitate, ethyl cellulose, hydroxypropyl-methylcellulose phthalate, hydroxypropylmethylcellulo se acetate succinate, carboxymethyl ethylcellulose, starch acetate phthalate, amylose acetate phthalate, polyvinyl acetate phthalate, polyvinyl butyrate phthalate, styrene-maleic acid copolymer, methyl-acrylate-methacrylic acid copolymer (MPM-05), methylacrylate-methacrylic acid-methylmethacrylate copolymer (MPM-06), and methylmethacrylate-methacrylic acid co-polymer (Eudragit® L &amp; S). Optionally, the enteric coating contains a plasticiser. Examples of the plasticiser include, but are not limited to, triethyl citrate, triacetin, and diethyl phthalate. 
     The salts of the disclosure may be combined and/or co-administered with any cardiovascular treatment agent. There are large numbers of cardiovascular treatment agents available In commercial use, in clinical evaluation and in pre-clinical development, which could be selected for use with a product of the disclosure for the prevention of cardiovascular disorders by combination drug therapy. Such agent can be one or more agents selected from, but not limited to several major categories, namely, a lipid-lowering drug, including an IBAT (ileal Na + /bile acid cotransporter) inhibitor, a fibrate, niacin, a sta tin, a CETP (cholesteryl ester transfer protein) inhibitor, and a bile acid sequestrant, an anti-oxidant, including vitamin E and probucol, a IIb/IIIa antagonist (e.g. abciximab, eptifibatide, tirofiban), an aldosterone inhibitor (e.g, spirolactone and epoxymexrenone), an adenosine A2 receptor antagonist (e.g. losartan), an adenosine A3 receptor agonist, a betablocker, acetylsalicylic acid, a loop diuretic and an ACE (angiotensin converting enzyme) inhibitor. 
     The salts of the disclosure may be combined and/or co-administered with any antithrombotic agent with a different mechanism of action, such as the antiplatelet agents acetylsalicylic acid, ticlopidine, clopidogrel, thromboxane receptor and/or synthetase inhibitors, prostacyclin mimetics and phosphodiesterase inhibitors and ADP-receptor (P 2  T) antagonists. 
     The products of the disclosure may further be combined and/or co-administered with thrombolytics such as tissue plasminogen activator (natural, recombinant or modified), streptokinase, urokinase, prourokinase, anisoylated plasminogen-streptokinase activator complex (APSAC), animal salivary gland plasminogen activators, and the like, in the treatment of thrombotic diseases, in particular myocardial infarction. 
     The salts of the disclosure may be combined and/or co-administered with a cardioprotectant, for example an adenosine A1 or A3 receptor agonist. 
     There is also provided a method for treating an inflammatory disease in a patient that comprises treating the patient with a product of the disclosure and an NSAID, e.g., a COX-2 inhibitor. Such diseases include but are not limited to nephritis, systemic lupus, erythematosus, rheumatoid arthritis, glomerulonephritis, vasculitis and sarcoldosis. Accordingly, the anti-thrombotic salts of the disclosure may be combined and/or co-administered with an NSAID. 
     Typically, therefore, the salts described herein may be administered to a host to obtain a thrombin-inhibitory effect, or in any other thrombin-Inhibitory or anti-thrombotic context mentioned herein. 
     Actual dosage levels of active ingredients in the pharmaceutical compositions of this disclosure may be varied so as to obtain an amount of the active compound(s) that is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration (referred to herein as a “therapeutically effective amount”). The selected dosage level will depend upon the activity of the particular compound, the severity of the condition being treated and the condition and prior medical history of the patient being treated. However, it is within the skill of the art to start doses of the compound at levels lower than required for to achieve the desired therapeutic effect and to gradually increase the dosage until the desired effect is achieved. 
     For example, it is currently contemplated that, in the case of oral administration of salts of TRI 50c, the salts might for instance be administered in an amount of from 0.5 to 2.5 mg/Kg twice daily, calculated as TRI 50c. Other salts might be administered in equivalent molar amounts. The disclosure is not limited to administration in such quantities or regimens and includes dosages and regimens outside those described in the previous sentence. 
     According to a further aspect of the disclosure there is provided an oral pharmaceutical formulation including a product of the disclosure, in admixture with a pharmaceutically acceptable adjuvant, diluent or carrier. 
     Solid dosage forms for oral administration include capsules, tablets (also called pills), powders and granules. In such solid dosage forms, the active compound is typically mixed with at least one inert, pharmaceutically acceptable exciplent or carrier such as sodium citrate or dicalcium phosphate and/or one or more: a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol and silicic acid; b) binders such as carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidone, sucrose and acacia; c) humectants such as glycerol; d) disintegrating agents such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates and sodium carbonate; e) solution retarding agents such as paraffin; f) absorption accelerators such as quaternary ammonium compounds; g) wetting agents such as cetyl alcohol and glycerol monostearate; h) absorbents such as kaolin and bentonite clay and i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate and mixtures thereof. In the case of capsules and tablets, the dosage form may also comprise buffering agents. Solid compositions of a similar type may also be employed as fillers In soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycol, for example. 
     Suitably, the oral formulations may contain a dissolution aid. The dissolution aid is not limited as to Its identity so long as it is pharmaceutically acceptable. Examples include nonionic surface active agents, such as sucrose fatty acid esters, glycerol fatty acid esters, sorbitan fatty acid esters (e.g., sorbitan trioleate), polyethylene glycol, polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene alkyl ethers, methoxypolyoxyethylene alkyl ethers, polyoxyethylene alkylphenyl ethers, polyethylene glycol fatty acid esters, polyoxyethylene alkylamines, polyoxyethylene alkyl thloethers, polyoxyethylene polyoxypropylene copolymers, polyoxyethylene glycerol fatty acid esters, pentaerythritol fatty add esters, propylene glycol monofatty acid esters, polyoxyethylene propylene glycol monofatty acid esters, polyoxyethylene sorbitol fatty acid esters, fatty acid alkylolamides, and alkylamine oxides; bile acid and salts thereof (e.g., chenodeoxycholic acid, cholic acid, deoxycholic acid, dehydrocholic acid and salts thereof, and glycine or taurine conjugate thereof); Ionic surface active agents, such as sodium laurylsulfate, fatty acid soaps, alkylsulfonates, alkylphosphates, ether phosphates, fatty add salts of basic amino acids; triethanolamine soap, and alkyl quaternary ammonium salts; and amphoteric surface active agents, such as betaines and aminocarboxylic acid salts. 
     The active compounds may also be in micro-encapsulated form, if appropriate, with one or more of the above-mentioned excipients. 
     Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups and elixirs. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly used in the art such as water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethyl formamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan and mixtures thereof. Besides inert diluents, the oral compositions may also include adjuvants such as, wetting agents, emulsifying and suspending agents, sweetening, flavouring and perfuming agents. Suspensions, in addition to the active compounds, may contain suspending agents such as ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminium metahydroxide, bentonite, agar-agar, and tragacanth and mixtures thereof. 
     The product of the disclosure may be presented as solids in finely divided solid form, for example they may be micronised. Powders or finely divided solids may be encapsulated. 
     The active compound may be given as a single dose, in multiple doses or as a sustained release formulation. 
     It will be understood from the aforegoing that there are provided pharmaceutical products comprising a metal salt, e.g. an alkali metal salt, of a boronic acid of Formula (I) in dry fine particle form, suitable FOR administration. The metal salt may be in a form appropriate for oral administration. The metal salt is suitably an acid salt. 
     Synthesis 
     1. Peptide/Peatidomimetic Synthesis 
     The synthesis of boropeptides, including, for example, Cbz-D-Phe-Pro-BoroMpg-OPinacol is familiar to those skilled in the art and described in the prior art mentioned above, including Claeson et al (U.S. Pat. No. 5,574,014 and others) and Kakkar et al (WO 92/07869 and family members including U.S. Pat. No. 5,648,338). It is described also by Elgendy et al  Adv. Exp. Med. Biol . ( USA ) 340:173-178, 1993; Claeson, G. et al  Biochem. J.  290:309-312, 1993; Deadman et a 11  Enzyme Inhibition  9:29-41, 1995, and by Deadman et al  J. Med. Chem.  38:1511-1522, 1995. 
     Stereoselective synthesis with S or R configuration at the chiral B-terminal carbon may be conducted using established methodology (Elgendy et al  Tetrahedron. Lett.  33:4209-4212, 1992; WO 92/07869 and family members including U.S. Pat. No. 5,648,338) using (+) or (−)-pinanediol as the chiral director (Matteson et al  J. Am. Chem. Soc.  108:810-819, 1986; Matteson et al  Organometallics.  3:1284-1288, 1984). Another approach is to resolve the requisite aminoboronate intermediate (e.g. Mpg-BOPinacol) to selectively obtain the desired (R)-isomer and couple It to the dipeptide moiety (e.g. Cbz-(R)-Phe-(S)-Pro, which is the same as Cbz-D-Phe-L-Pro) which will form the remainder of the molecule. 
     The boropeptides may be synthesised initially in the form of boronic acid esters, particularly esters with diols. Such diol esters may be converted to the peptide boronic acid as described next. 
     2. Ester to Acid Conversion 
     A peptide boronate ester such as Cbz-(R)-Phe-Pro-BoroMpg-OPinacol may be hydrolysed to form the corresponding acid. 
     A novel technique for converting a dial ester of a peptide boronic acid of formula (I) into the acid comprises dissolving the diol ester in an ether and particularly a dialkyl ether, reacting the thus-dissolved diol with a diolamine, for example a dialkanolamine, to form a product precipitate, recovering the precipitate, dissolving it in a polar organic solvent and reacting the thus-dissolved product with an aqueous medium, e.g. an aqueous acid, to form the peptide boronic acid. The boronic acid may be recovered from the organic layer of the mixture resulting from the reaction, for example by removing the solvent, e.g. by evaporation under vacuum or distillation. The reaction between the diol ester and the diolamine may be carried out under reflux, for example. 
     The identity of the dial is not critical. As suitable dials may be mentioned aliphatic and aromatic compounds having hydroxy groups that are substituted on adjacent carbon atoms or on carbon atoms substituted by another carbon. That is to say, suitable diols include compounds having at least two hydroxy groups separated by at least two connecting carbon atoms in a chain or ring. One class of diols comprises hydrocarbons substituted by exactly two hydroxy groups. One such diol is pinacol and another is pinanediol; there may also be mentioned neopentylglycol, 1,2-ethanediol, 1,2-propanediol, 1,3-propanediol, 2,3-butanediol, 1,2-diisopropylethanediol, 5,6-decanediol and 1,2-dicyclohexylethanediol. 
     The alkyl groups of the dialkyl ether preferably have 1, 2, 3 or 4 carbon atoms and the alkyl groups may be the same or different. An exemplary ether is diethyl ether. 
     The alkyl groups of the dialkanolamine preferably have 1, 2, 3 or 4 carbon atoms and the alkyl groups may be the same or different. An exemplary dialkanolamine is diethanolamine. The diethanolamine/boronic acid reaction product hydrolyses in water at room temperature and the rate of hydrolysis may be accelerated by adding acid or base. 
     The polar organic solvent is preferably CHCl 3 . Other examples are polyhalogenated alkanes generally and ethyl acetate. In principle, any polar organic solvent is acceptable other than alcohols. 
     The aqueous acid is suitably a strong inorganic acid at a pH in the region of 1 such as hydrochloric acid, for example. 
     After reaction with the acid, the reaction mixture is suitably washed with, for example, NH 4 Cl or another mild base. 
     An example of a specific procedure is as follows
         1. The pinacol or pinanediol ester of the selected peptide boronic acid is dissolved in diethylether.   2. Diethanolamine is added and the mixture is refluxed at 40° C.   3. The precipitated product is removed (filtered), washed (usually several times) with diethyl ether or another polar organic solvent other than an alcohol, and dried (e.g. by evaporation under vacuum).   4. The dry product is dissolved in a polar organic solvent other than an alcohol, e.g. CHCl 3 . Aqueous acid or base is added, e.g hydrochloric acid (pH 1), and the mixture is stirred for e.g. approximately 1 h at room temperature.   5. The organic layer is removed and washed with NH 4 Cl solution.   6. The organic solvent is distilled off and the residual solid product is dried.       

     The above process results in the formation of what may conveniently be referred to as a “diolamine adduct” of the peptide boronic acids of formula (I), especially such adducts with diethanolamine, and such adducts are themselves included in the disclosure. The molecular structure of such adducts is not known: they might comprise a compound in which the two oxygens and the nitrogen of the diolamine are all coordinated to the boron; they might comprise ions. The adducts are however considered to be esters. A particular novel product included In the disclosure is that obtainable by reacting a pinacol or pinanediol ester of a compound of Formula VIII, particularly (R,S,R)-TRI 50c, and diethanolamine, i.e. the novel product is an (R,S,R)-TRI 50c/diethanolamine “adduct” where the acid Is (R,S,R)-TRI 50c. 
     The diolamine materials of the disclosure may be defined as a composition of matter comprising: 
     
       
         
         
             
             
         
       
     
     wherein X is H or an amino protecting group, the boron atom is optionally coordinated additionally with a nitrogen atom, and the valency status of the terminal oxygens is open (they may be attached to a second covalent bond, be ionised as —O—, or have some other, for example intermediate, status); and, in bonding association therewith 
     
       
         
         
             
             
         
       
     
     wherein the valency status of the nitrogen atom and the two oxygen atoms is open. It will be appreciated that the terminal oxygen atoms of the species of formula (IX) and the oxygen atoms of the species of formula (X) may be the same oxygen atoms, in which case the species of formula (X) forms a diol ester with the species of formula (IX). 
     It will be appreciated that the aforegoing technique comprises an example of a method for recovering an organoboronic acid product, the method comprising providing in a solvent a dissolved mixture comprising the organoboronic acid in a soluble form and a compound having two hydroxy groups and an amino group (i.e. a diolamine), causing or allowing the organoboronic acid and the diolamine to react to form a precipitate, and recovering the precipitate. The soluble form of the organoboronic acid may be a diol ester, as discussed above. The solvent may be an ether, as discussed above. The organoboronic acid may be one of the organoboronic acids referred to in this specification, for example it may be of Formula (I), (II) or (III). The method described in this paragraph is novel and forms an aspect of the disclosure. A recovery method is filtration. 
     The reaction between the diolamine and the soluble form of the organoboronic acid is suitable carried out at an elevated temperature, for example under reflux. 
     Another aspect of the disclosure is a method for recovering an organoboron species, comprising 
     providing, in a form soluble in an ether, an organoboronic acid, for example a drug such as, e.g., a compound of formula (III); 
     forming a solution of the soluble form in the ether; 
     combining the solution with a dialkanolamine and allowing or causing the dialkanolamine to react with the soluble form of the organoboronic acid to form an insoluble precipitate; and recovering the precipitate. 
     The term “soluble” in the preceding paragraph refers to species which are substantially more soluble in the reaction medium than is the precipitated product. In variants of the method, the ether is replaced by toluene or another aromatic solvent. 
     The diethanolamine precipitation technique described above is an example of another novel method, which is a method for recovering from ether solution a pinacol or pinanediol ester of a peptide boronic acid, comprising dissolving diethanolamine in the solution, allowing or causing a precipitate to form and recovering the precipitate. The disclosure encompasses variants of this methods in which another diol than pinacol or pinanediol is used. 
     The precipitated material, i.e. the “adduct”, may be converted into the free organoboronic acid, for example by contacting it with an acid. The acid may be an aqueous acid, for example an aqueous inorganic acid, e.g. as described above. The precipitate may be dissolved, for example in an organic solvent, prior to being contacted with the acid. 
     The disclosure therefore provides a method for making an organoboronic acid, comprising converting its diolamine reaction product to the acid. 
     The acid resulting from the methods described in the previous two paragraphs may be converted to a salt of the acid with a multivalent metal, which salt may in turn be formulated into a pharmaceutical composition in oral dosage form. 
     3. Salt Synthesis 
     In general, the salts may be prepared by contacting the relevant peptide boronic acid with a strong base appropriate to form the desired salt. In the case of metal salts, the metal hydroxides are suitable bases (alternatively, metal carbonates might be used, for example), whilst sometimes it is more convenient to contact the acid with a relevant metal alkoxide (eg methoxide), for which purpose the corresponding alkanol is a suitable solvent. Salts with organic bases may be prepared by contacting the peptide boronic acid with the organic base itself. Illustrative salts are acid salts (one —BOH proton replaced) and, to make acid salts with a monovalent cation, the acid and the base are suitably reacted in substantially equimolar quantities. Generally stated, therefore, the usual acid:base molar ratio is substantially n:1, where n is the valency of the cation of the base. 
     In one procedure, a solution of the peptide boronic acid in a water-miscible organic solvent, for example acetonitrile or an alcohol (e.g. ethanol, methanol, a propanol, for example iso-propanol, or another alkanol), is combined with an aqueous solution of the base. The acid and the base are allowed to react and the salt is recovered. The reaction is typically carried out at ambient temperature (e.g. at a temperature of from 15 to 30° C., e.g. 15 to 25° C.), but an elevated temperature may be used, for example up to the boiling point of the reaction mixture but more usually lower, e.g. a temperature of up to 40° C. or 50° C. The reaction mixture may be allowed to stand or be agitated (usually stirred). 
     The time during which the acid and the base are allowed to react is not critical but it has been found desirable to maintain the reaction mixture for at least one hour. A period of from one to two hours is usually suitable but longer reaction times may be employed. 
     The salt may be recovered from the reaction mixture by any suitable method, for example evaporation or precipitation. Precipitation may be carried out by adding an excess of a miscible solvent in which the salt has limited solubility. In one preferred technique, the salt is recovered by evacuating the reaction mixture to dryness. The salt is preferably thereafter purified, for example by redissolving the salt before filtering the resulting solution and drying it, for example by evacuating it to dryness. The redissolution may be performed using water, e.g. distilled water. The salt may then be further purified, for example in order to remove residual water by further redissolution in a suitable solvent, which is advantageously ethyl acetate or THF followed by evaporating to dryness. The purification procedure may be carried out at ambient temperature (say, 15 to 30° C., e.g. 1.5 to 25° C.), or at a modestly elevated temperature, such as e.g. a temperature not exceeding 40° C. or 50° C.; for example the salt may be dissolved in water and/or solvent by agitating with or without warming to, for example, 37° C. 
     Also included is a method for drying the salts of the disclosure and other peptide boronic acid salts, comprising dissolving them in an organic solvent, e.g. ethyl acetate or THF, and then evaporating to dryness, e.g. by evacuation. 
     Generally, preferred solvents for use in purifying the salts are ethyl acetate or THF, or perhaps another organic solvent. 
     A general procedure for synthesising salts of Cbz-Phe-Pro-BoroMpg-OH is as follows: 
     Cbz-Phe-Pro-BoroMpg-OH (20.00 g, 38.1 mM) is dissolved in acetonitrile (200 ml) with stirring at room temperature. To this solution is added the requisite base in solution in distilled water (190 ml); the base is added as a 0.2M solution for a monovalent cation. The resultant clear solution is allowed to react for example by being left to stand or being agitated, for a usual period, in either case, of from one to two hours. The reaction is typically carried out at ambient temperature (e.g. 15-30° C., e.g. 15 to 25° C.) but alternatively the temperature may be elevated (e.g. up to 30° C., 40° C. or 50° C.). The reaction mixture is then evacuated to dryness under vacuum with its temperature not exceeding 37° C., typically to yield a white brittle solid or an oil/tacky liquid. The oil/tacky liquid is redissolved in the minimum amount of distilled water necessary (200 ml to 4 L), typically with warming (e.g. to 30-40° C.), usually for up to 2 hours. The solution is filtered, suitably through filter paper, and evacuated to dryness, again with the temperature of the solution not exceeding 37° C., or freeze dried. The resultant product is dried under vacuum overnight to normally yield a white brittle solid. If the product is present as an oil or tacky solid then it is dissolved in ethyl acetate and evacuated to dryness to produce the product as a white solid. The white solid is typically a coarse, amorphous powder. 
     In variations of the aforegoing general procedure, the acetonitrile is replaced by another water-miscible organic solvent, notably an alcohol, as discussed above, especially ethanol, methanol, isopropanol or another propanol. 
     Where a boronic acid salt is less soluble in a selected reaction medium for salt formation such that its direct preparation from the corresponding acid and base is inconvenient, the less soluble salt may be prepared from a salt more soluble in the reaction medium. 
     There is provided also the use of a boronic acid to make a salt of the disclosure. Included also is a method of preparing a product of the disclosure, comprising contacting a boronic acid, e.g. of formula (I), (II) or (III), with a base capable of making such a salt. 
     The peptide boronic acid of formula (I) used to prepare the pharmaceutical preparations is typically of GLP or GMP quality, or in compliance with GLP (good laboratory practice) or GMP (good manufacturing practice); such acids are included in the disclosure. 
     Similarly the acids are usually sterile and/or acceptable for pharmaceutical use, and one aspect of the disclosure reside in a composition of matter which is sterile or acceptable for pharmaceutical use, or both, and comprises a peptide boronic acid of formula (I). Such a composition of matter may be in particulate form or in the form of a liquid solution or dispersion. 
     The intermediate acid may be in isolated form and such isolated acids are included in the disclosure, especially isolated adds which are a peptide boronic acid of formula (VIII): 
       X—(R)-Phe-(S)-Pro-(R)-Mpg-B(OH) 2   (VIII) 
     wherein X is H (to form NH 2 ) or an amino-protecting group. 
     One typical way of providing the intermediate acids is as a particulate composition consisting predominantly of such a peptide boronic acid, and these compositions are included in the disclosure. The peptide boronic acid often forms at least 75% by weight of the composition and typically at least 85% by weight of the composition, e.g. at least 95% by weight of the composition. 
     Another typical way of providing the intermediate acids is as a liquid composition consisting of, or consisting essentially of, a peptide boronic acid of formula (II) and a liquid vehicle in which it is dissolved or suspended. The liquid vehicle may be an aqueous medium, e.g. water, or an alcohol, for example methanol, ethanol, isopropanol, or another propanol, another alkanol or a mixture of the aforegoing. 
     The compositions of the intermediate acids are generally sterile. The compositions may contain the peptide boronic acid in finely divided form, to facilitate further processing. 
     Separation of Stereoisomers 
     The stereoisomers of a peptide boronic ester or a synthetic intermediate aminoboronate may be resolved in, for example, any known way. In particular, stereoisomers of boronic esters may be resolved by HPLC. 
     EXAMPLES 
     Examples 1 to 3 
     Introductory Remarks 
     Apparatus 
     Throughout the following procedures of Examples 1 to 3 and 33, standard laboratory glassware and, where appropriate, specialised apparatus for handling and transferring of air sensitive reagents are used. 
     All glassware is heated at 140-160° C. for at least 4 hours before use and then cooled either in a desiccator or by assembling hot and purging with a stream of dry nitrogen. 
     Solvents 
     The organic solvents used in the procedures of Examples 1 to 3 and 33 are all dry. Suitably, they are dried over sodium wire before use. 
     Dryness 
     In the drying procedures of Example 1 to 3 and 33, products are tested for dryness (including dryness in terms of organic solvent) by observing weight loss on drying. The following procedure was followed to determine loss on drying: a sample was placed in a vacuum drier and dried at 40° C. at 100 mbar for 2 hours. Products are considered dry when the decrease in weight upon drying is less than 0.5% of the total weight of the starting material. 
     Examples 1 to 3 and 33 describe performance of the following reaction scheme and conversion of the resultant TRI 50c to sodium and calcium salts thereof: 
     
       
         
         
             
             
         
       
     
     Example 1 
     Synthesis of TRI 50D 
     Step 1: Z-DIPIN B 
     Procedure A 
     17.8 g (732.5 mmole) magnesium turnings, 0.1 g (0.4 mmole) iodine and 127 ml dry tetrahydrofuran are charged and heated to reflux. Then 15 ml of a solution of 66 g (608 mmole) 1-chloro-3-methoxypropane in 185 ml dry tetrahydrofuran are added and stirred under reflux until the vigorous reaction starts. After the initial exotherm ceases, the solution of 1-chloro-3-methoxypropane is added slowly to maintain gentle reflux until all the magnesium is consumed, After the reaction is finished, the reaction mixture is cooled to ambient temperature and slowly added to a solution of 64.4 g (620 mmole) trimethylborate in 95 ml dry tetrahydrofuran; the latter solution is cooled to below 0° C. and, if it warms up during the course of the reaction, the reaction mixture must be added to it sufficiently slowly to maintain the temperature of this solution below 65° C. Upon complete addition, the reaction mixture is allowed to warm to about 0° C. and stirred for another 60 minutes. Then a solution of 22.4 ml sulfuric acid in 400 ml water is added slowly so as to maintain the temperature below 20° C. The layers are allowed to settle and the phases are separated. The aqueous layer is rewashed three times with 200 ml tert-butylmethylether. The combined organic layers are allowed to settle and additional water separated from this solution is removed. The organic layer is dried over magnesium sulfate, filtered and evaporated to dryness. The evaporation residue is filtered from the precipitated solid and the filtrate dissolved in 175 ml toluene. 34.8 g (292 mmole) pinacol is charged to the solution followed by stirring at ambient temperature for not less than 10 hours. The solution is evaporated to dryness, dissolved in 475 ml n-heptane and washed three times with 290 ml saturated aqueous solution of sodium hydrogen carbonate. The n-heptane solution is evaporated to dryness and the evaporation residue distilled and the fraction with Bp 40-50° C. at 0.1-0.5 mbar recovered. 
     Boiling point: 40-50° C./0.1-0.5 mbar 
     Yield: 40.9 g (70%) Z-DIPIN B (oil) 
     Procedure B 
     17.8 g (732.5 mmole) magnesium turnings, 0.1 g (0,4 mmole) iodine and 127 ml dry tetrahydrofuran are charged and heated to reflux. Then 15 ml of a solution of 66 g (608 mmole) 1-chloro-3-methoxypropane in 185 ml dry tetrahydrofuran are added and stirred under reflux until the vigorous reaction starts. After the initial exotherm ceases, the solution of 1-chloro-3-methoxypropane is added slowly to maintain gentle reflux. After the reaction is finished, the reaction mixture is cooled to ambient temperature and slowly added to a solution of 64.4 g (620 mmole) trimethylborate in 95 ml dry tetrahydrofuran, maintaining the temperature of this solution below minus 65° C. Upon complete addition, the reaction mixture is allowed to warm to about 0° C. and stirred for another 60 minutes. Then a solution of 22.4 ml sulfuric acid in 400 ml water is added slowly so as to maintain the temperature below 20° C. The organic solvent is removed by distillation under vacuum. 300 ml n-heptane is charged to the aqueous solution of the evaporation residue followed by addition of 34.8 g (292 mmole) pinacol. The two-phase-mixture is stirred at ambient temperature for not less than 2 hours. After allowing the layers to settle, the aqueous phase is separated. 300 ml n-heptane is charged to the aqueous solution and the two-phase-mixture is stirred at ambient temperature for not less than 2 hours. After allowing the layers to settle, the aqueous phase is separated. The organic layers are combined and washed once with 200 ml water, followed by 200 ml saturated sodium hydrogen carbonate solution and two further washes with 200 ml water each. The n-heptane solution is evaporated to dryness and the evaporation residue distilled and the fraction with Bp 40-50° C. at 0.1-0.5 mbar recovered. 
     Boiling point: 40-50° C./0.1-0.5 mbar 
     Yield: 40.9 g (70-85%) Z-DIPIN B (oil) 
     Step 2: Z-DIPIN C 
     16.6 g (164 mmole) dilsopropylamine and 220 ml tetrahydrofuran are charged and cooled to −30 to −40° C. To this solution 41.8 g (163 mmole) n-butyl lithium, 25% in n-heptane is added, followed by stirring at 0 to −5° C. for one hour. This freshly prepared solution of lithium diisopropylamide is cooled to −30° C. and then added to a solution of 27.9 g (139 mmole) Z-DIPIN B in 120 ml tetrahydrofuran and 35.5 g (418 mmole) dichloromethane at a temperature between −60 and −75° C. The solution is stirred at that temperature for half an hour followed by addition of 480 ml (240 mmole) 0.5 N anhydrous Zinc(II)-chloride in tetrahydrofuran or 32.5 g (240 mmole) anhydrous solid Zinc(II)-chloride. After stirring at −65° C. for one hour, the reaction mixture is allowed to warm to ambient temperature and stirred for another 16-18 hours. The reaction mixture is evaporated to dryness (i.e, until solvent is removed) and followed by addition of 385 ml n-heptane. The reaction mixture is washed with 150 ml 5% sulfuric acid, with 190 ml saturated sodium hydrogen carbonate solution, and 180 ml saturated sodium chloride solution. The organic layer is dried over magnesium sulfate, filtered and evaporated to dryness (i.e. until solvent is removed). The oily residue is transferred into the next step without further purification. 
     Yield: 19 g (55%) Z-DIPIN C 
     Step 3: Z-DIPIN D 
     To a solution of 23.8 g (148 mmole) hexamethyldisilazane in 400 ml tetrahydrofuran at −15° C. is added 34.7 g (136 mmole) n-butyl lithium, 25% in n-heptane and stirred for one hour. The solution is cooled to −55° C. followed by the addition of 30.6 g (123 mmole) Z-DIPIN C dissolved in 290 ml tetrahydrofuran and 35 ml tetrahydrofuran to this freshly prepared solution of LIHMDS. The solution is allowed to warm to ambient temperature and stirred for 12 hours. The reaction mixture is evaporated to dryness, the evaporation residue dissolved in 174 ml n-heptane, washed with 170 ml water and 75 ml saturated sodium chloride solution. The organic phase Is dried over magnesium sulfate, filtered and evaporated to complete dryness (i.e. until solvent is removed). The oily residue is dissolved in 100 g n-heptane. This solution is carried over into the next step without further purification. 
     Yield: 32.2 g (70%) Z-DIPIN D 
     Step 4: Z-DIPIN (TRI50b, Crude) 
     A solution of 26.6 g (71 mmole) Z-DIPIN D in 82.6 g n-heptane is diluted with 60 ml n-heptane and cooled to −60° C. followed by introduction of 10.5 g (285 mmole) hydrogen chloride. The reaction mixture is subsequently evacuated and flushed with nitrogen, while the temperature is increased in increments of about 20° C. to ambient temperature. The solvent is removed from the oily precipitate and replaced several times by 60 ml fresh n-heptane. The oily residue is dissolved in 60 ml tetrahydrofuran (Solution A). 
     To a different flask 130 ml tetrahydrofuran, 24.5 g (61.5 mmole) Z-D-Phe-Pro-OH and 6.22 g (61.5 mmole) N-methylmorpholine are charged and cooled to −20° C. To this solution a solution of 8.4 g (61.5 mmole) isobutylchloroformate in 20 ml tetrahydrofuran Is added and stirred for 30 minutes, followed by addition of Solution A at −25° C. Upon complete addition, up to 16 ml (115 mmole) trlethylamine is added to adjust the pH to 9-10, measured using a pH stick. The reaction mixture is allowed to warm to ambient temperature and stirred for 3 hours, still under nitrogen. The solvent is evaporated to dryness and the evaporation residue dissolved in 340 ml tert.-butylmethylether (t-BME). The solution of Z-DIPIN in t-BME is washed twice with 175 ml 1.5% hydrochloric acid. The combined acidic washes are given a rewash with 175 ml t-BME. The combined organic layers are washed with 175 ml water, with 175 ml saturated sodium hydrogen carbonate solution, with 175 ml 25% sodium chloride solution, dried over magnesium sulfate and filtered. This solution is carried over into the next step without further purification. 
     Yield: 29.9 g (80%) Z-DIPIN 
     Example 2 
     Synthesis of TRI 50D (Diethanolamine Adduct of TRI 50C) 
     The starting material used in this Example is the solution of TRI 50b (“Z-DIPIN”) obtained in Example 1. The solution is carried forward to the synthesis of TRI 50d without further purification. The solution of Z-DIPIN in t-BME (containing 7.0 g (11.5 mmole) (R,S,R) TRI 50b, calculated based on HPLC results of Z-DIPIN) Is evaporated to dryness and the evaporation residue dissolved in BO ml diethylether, 1.51 g (14.4 mmole) diethanolamine is added and the mixture heated at reflux for at least 10 hours, during which process the product precipitates. The suspension is cooled to 5-10° C., filtered and the filter residue washed with diethylether. 
     To improve chiral and chemical purity the wet filter cake (7 g) is dissolved in 7 ml dichloromethane, cooled to 0-5° C. and the product precipitated by addition of 42 ml diethylether and filtered. The isolated wet product is dried at 35° C. in vacuum or at least 4 hours, until day. 
     Yield: 5.5 g (80%) Tri50d 
     Melting Point: 140-145° C. 
     Example 3 
     Preparation of Sodium Salt of TRI50C 
     1.5 kg (2.5 mole) TRI50d Example 2 is dissolved in 10.5 L dichloromethane. 11. L 2% hydrochloric acid is added and the mixture is stirred for at most 30 minutes (optimally about 20 minutes) at room temperature. A precipitate forms in the organic phase. After stirring, the layers are allowed to settle and separated. The aqueous layer is rewashed twice with 2.2 L dichloromethane. The combined organic layers are washed with a solution of 625 g ammonium chloride in 2.25 L water. (The ammonium chloride buffers the pH of the aqueous extractions to be within a range of from about pH 1-2 to about pH 4-5, as strongly acidic conditions might cleave peptide bonds). The organic phase is dried over magnesium sulfate, filtered and the filtrate evaporated to dryness. An assay of the free boronic acid is performed (by the RP HPLC method of Example 28 for at most 30 mins (optionally about 20 min) at room temperature) and the amounts of the solvents and base for conversion of the acid to the salt are calculated. If 2.5 mol of the free acid is obtained, the evaporation residue is dissolved in 5 L acetonitrile followed by addition of a solution of 100 g (2.5 mole) sodium hydroxide as a 5% solution in 2.2 L water. The solution is stirred for two hours at ambient temperature (e.g. 15-30° C., optimally room temperature) and then evaporated in vacuum (of ca. 10 mmHg) at a temperature not exceeding 35° C. The evaporation residue is repeatedly dissolved in 3.5 L fresh acetonitrile and evaporated to dryness to remove traces of water. If the evaporation residue is dry, it is dissolved in 3 L acetonitrile (or alternatively in 6 L THF) and slowly added to a mixture of 32 L n-heptane and 32 L diethylether. The addition is performed slowly enough to avoid lumping or sticking of the product and is carried out over a period of not less than 30 minutes. The precipitated product is filtered off, washed with n-heptane and dried under vacuum at a temperature initially of about 10° C. and then increasing to a limit of about 35° C.:, until dry. 
     Yield: 1.0 kg (70%) Tri50c sodium salt. 
     Example 4 
     Alternative Conversion of TRI 50B to TRI 50C 
     The synthetic procedures described in this and subsequent synthetic examples were generally performed under nitrogen and using dry solvents as supplied from commercial sources. 
     1. Approximately 300 g of TRI 50b, obtained by the HPLC purification of racemic TRI 50b, were dissolved in approximately 2.5 L diethylether. It is estimated that different batches of TRI 50b had isomeric purities ranging from 85% R,S,R to in excess of 95% R,S,R. 
     2. Approximately 54 ml diethanolamine were added (1:1 stolchiometry with total TRI 50b content), and the mixture was refluxed at 40° C. 
     3. The precipitated product was removed, washed several times with diethylether and dried. 
     4. The dry product was dissolved in CHCl 3 . Hydrochloric acid (pH 1) was added and the mixture was stirred approximately 1 h at room temperature. 
     5. The organic layer was removed and washed with NH 4 Cl solution. 
     6. The organic solvent was distilled off and the residual solid product was dried. 
     Typical yield: Approximately 230 g 
     Example 5 
     Preparation of Lithium Salt of TRI 50C 
     Cbz-Phe-Pro-BoroMpg-OH obtained by the method of Example 4 (20.00 g, 38.1 mM) is dissolved in acetonitrile (200 ml) with stirring at room temperature. To this solution is added LiOH as a 0.2M solution in distilled water (190 ml). The resultant clear solution is stirred for 2 hours at room temperature and then evacuated to dryness under vacuum with its temperature not exceeding 37° C. The resultant oil/tacky liquid is redissolved in 500 ml distilled water necessary with light warming for about 20 minutes. The solution is filtered through filter paper and evacuated to dryness, again with the temperature of the solution not exceeding 37° C. The resultant product is dried under vacuum overnight to normally yield a white brittle solid. 
     The salt was then dried under vacuum over silica to constant weight (72 h). 
     Yield 17.89 g. 
     Microanalysis: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 C % Found 
                 H % Found 
                 N % Found 
                 B % Found 
                 Metal % Found 
               
               
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
                 (Calc,) 
                 (Calc.) 
               
               
                   
               
             
            
               
                 57.14 
                 6.60 
                 7.34 
                 2.07 
                 Li 1.26 
               
               
                 (61.03) 
                 (6.64) 
                 (7.90) 
                 (2.03) 
                 (1.31) 
               
               
                   
               
            
           
         
       
     
     Example 6 
     UV/Visible Spectra of Lithium Salt of TRISOC 
     UV/Visible spectra of the salt resulting from the procedure of Example 5 were recorded in distilled water at 20° C. from 190 nm to 400 nm. The salt gave λ max  at 210 and 258 nm. The weight of the dried salt was then measured for the purposes of calculating the extinction coefficient. The λ max  at 258 nm was used. The extinction coefficient was calculated using the formula:—
         A=εc1 where A is the absorbance   C is the concentration   1 the path length of the UV cell   and ε is the extinction coefficient.   Extinction coefficient: 451       

     Example 7 
     Aqueous Solubility of Lithium Salt of TRI5OC 
     The salt used in this Example was made using a modification of the process described in Example 5. The modified process differs from that described in that 100 mg of TRI 50c was used as starting material, the product of the redissolution in water was dried by freeze drying and the filtration was carried out through a 0.2 μm filter. The salt is believed to contain about 85% of R,S,R isomer. 
     To determine maximum aqueous solubility 25 mg of the dried salt were shaken in water at 37° C., the sample filtered and the UV spectrum measured. The salt left a white residue of undissolved material. The lithium salt was comparatively soluble and so was redissolved at 50 mg/ml in the same manner previously described. 
     Solubility when dissolved at 25 mg/ml: 43 mM (23 mg/ml). 
     Solubility when dissolved at 50 mg/ml: 81 mM (43 mg/ml). 
     Example 8 
     Preparation of Sodium Salt of TRI50C (TGN 2551 
     Cbz-Phe-Pro-BoroMpg-OH obtained by the method of Example 4 (20.00 g, 38.1 mM) is dissolved in acetonitrile (200 ml) with stirring at room temperature. To this solution is added NaOH as a 0.2M solution in distilled water (190 ml). The resultant clear solution is stirred for 2 hours at room temperature and then evacuated to dryness under vacuum with its temperature not exceeding 37° C. The resultant oil/tacky liquid is redissolved in 500 ml distilled water with light warming for about 15-20 minutes. The solution is filtered through filter paper and evacuated to dryness, again with the temperature of the solution not exceeding 37° C. The resultant product is dried under vacuum overnight to normally yield a white brittle solid. The product may be present as an oil or tacky solid due to residual water, in which case it is dissolved in ethyl acetate and evacuated to dryness to produce the product as a white solid. 
     The salt was then dried under vacuum over silica to constant weight (72 h). 
     Yield: Over 50%. 
     Microanalysis: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 C % Found 
                 H % Found 
                 N % Found 
                 B % Found 
                 Metal % 
               
               
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
               
               
                   
               
             
            
               
                 59.93 
                 6.47 
                 7.31 
                 1.91 
                 Na 3.81 
               
               
                 (59.24) 
                 (6.44) 
                 (7.67) 
                 (1.98) 
                 (4.20) 
               
               
                   
               
            
           
         
       
     
     Example 9 
     UV/Visible Spectra of Sodium Salt of TRI50C 
     UV/Visible spectra of the sodium salt resulting from the procedure of Example 8 were recorded In distilled water at 20° C. from 190 nm to 400 nm. The salt gave λ max  at 210 and 258 nm. The weight of the dried salt was then measured for the purposes of calculating the extinction coefficient. The λ max  at 258 nm was used. The extinction coefficient was calculated using the formula:—
         A=εc1 where A is the absorbance   C is the concentration   1 the path length of the UV cell   and ε is the extinction coefficient.   Extinction coefficient: 415.       

     Example 10 
     Aqueous Solubility of Sodium Salt of TRI50C 
     The salt used in this Example was made using a modification of the process described in Example 8. The modified process differs from that described in that 100 mg of TRI 50c was used as starting material, the product of the redissolution in water was dried by freeze drying and the filtration was carried out through a 0.2 pm filter. The salt is believed to contain about 85% of R,S,R isomer. 
     To determine maximum aqueous solubility 25 mg of the dried salt were shaken in water at 37° C., the sample filtered and the UV spectrum measured. The salt left a white residue of undissolved material. The sodium salt was comparatively soluble and so was redissolved at 50 mg/ml in the same manner previously described. 
     Solubility when dissolved at 25 mg/ml: 44 mM (25 mg/ml). 
     Solubility when dissolved at 50 mg/ml: 90 mM (50 mg/ml). 
     Example 11 
     Preparation of Potassium Salt of TRI50C 
     Cbz-Phe-Pro-BoroMpg-OH obtained by the method of Example 4 (20.00 g, 38.1 mM) is dissolved in acetonitrile (200 ml) with stirring at room temperature. To this solution is added KOH as a 0.2M solution in distilled water (190 ml). The resultant clear solution is stirred for 2 hours at room temperature and then evacuated to dryness under vacuum with its temperature not exceeding 37° C. The resultant oil/tacky liquid is redissolved in 1 L distilled water with warming to 37° C. for about 2 hours. The solution is filtered through filter paper and evacuated to dryness, again with the temperature of the solution not exceeding 37° C. The resultant product is dried under vacuum overnight to normally yield a white brittle solid. 
     Yield: 14.45 mg. 
     The salt was then dried under vacuum over silica to constant weight (72 h). 
     Microanalysis: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 C % Found 
                 H % Found 
                 N % Found 
                 B % Found 
                 Metal % 
               
               
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
               
               
                   
               
             
            
               
                 54.84 
                 6.25 
                 7.02 
                 2.01 
                 K 4.29 
               
               
                 (57.55) 
                 (6.26) 
                 (7.45) 
                 (1.92) 
                 (6.94) 
               
               
                   
               
            
           
         
       
     
     Example 12 
     UV/Visible Spectra of Potassium Salt of TRI50C 
     UV/Visible spectra of the potassium salt resulting from the procedure of Example 11 were recorded in distilled water at 20° C. from 190 nm to 400 nm. TRI50C and the salt gave λ max  at 210 and 258 nm. The weight of the dried salt was then measured for the purposes of calculating the extinction coefficient. The λ max  at 258 nm was used. The extinction coefficient was calculated using the formula:—
         A=εc1 where A is the absorbance   C is the concentration   I the path length of the UV cell   and ε is the extinction coefficient.   Extinction coefficient: 438.       

     Example 13 
     Aqueous Solubility of Potassium Salt of TRI50C 
     The salt used in this Example was made using a modification of the process described in Example 11. The modified process differs from that described in that 100 mg of TRI 50c was used as starting material, the product of the redissolution in water was dried by freeze drying and the filtration was carried out through a 0.2 μm filter. The salt is believed to contain about 85% of R,S,R isomer. 
     To determine maximum aqueous solubility 25 mg of the dried salt were shaken in water at 37° C., the sample filtered and the UV spectrum measured. The salt left a white residue of undissolved material. 
     Solubility when dissolved at 25 mg/ml: 29 mM (16 mg/ml). 
     Example 14 
     Preparation of Zinc Salt of TRI 50C 
     The relative solubility of zinc hydroxide is such that, if the hydroxide had been used to prepare the corresponding TRI 50c salt using the procedure of Example 5, they would not have resulted In homogeneous salt formation. A new method was therefore developed to prepare the zinc salt, as described in this and the next examples. 
     TRI 50c sodium salt (2.24 g, 4.10 mM) was dissolved in distilled water (100 ml) at room temperature and zinc chloride in THE (4.27 ml, 0.5M) was carefully added with stirring. A white precipitate that immediately formed was filtered off and washed with distilled water. This solid was dissolved in ethyl acetate and washed with distilled water (2×50 ml). The organic solution was evacuated to dryness and the white solid produced dried over silica in a desiccator for 3 days before microanalysis. Yield 1.20 g. 
       1 H NMR 400 MHz, δ H (CD 3 OD) 7.23-7.33 (20H, m, ArH), 5.14 (4H, m, PhCH 2 O), 4.52 (4H, m, αCH), 3.65 (2H, m), 3.31 (12H, m), 3.23 (6H, s, OCH 3 ), 2.96 (4H, d, J=7.8 Hz), 2.78 (2H, m), 2.58 (2H, m), 1.86 (6H, m), 1.40 (10H, m). 
       13 C NMR 75 MHz δc(CD 3 OD) 178.50, 159.00, 138.05, 137.66, 130.54, 129.62, 129.50, 129.07, 128.79, 128.22, 73.90, 67.90, 58.64, 58.18, 56.02, 38.81, 30.06, 28.57, 28.36, 25.29. 
     FTIR (KBr disc) v max  (cm −1 ) 3291.1, 3062.7, 3031.1, 2932.9, 2875.7, 2346.0, 1956.2, 1711.8, 1647.6, 1536.0, 1498.2, 1452.1, 1392.4, 1343.1, 1253.8, 1116.8, 1084.3, 1027.7, 916.0, 887.6, 748.6, 699.4, 595.5, 506.5. 
     Example 15 
     Preparation of Arginine Salt of TRI50C 
     Cbz-Phe-Pro-BoroMpg-OH obtained by the method of Example 4 (20.00 g, 38.1 mM) is dissolved in acetonitrile (200 ml) with stirring at room temperature. To this solution is added arginine as a 0.2M solution in distilled water (190 ml). The resultant clear solution is stirred for 2 hours at room temperature and then evacuated to dryness under vacuum with its temperature not exceeding 37° C. The resultant oil/tacky liquid is redissolved in 2 L distilled water with warming to 37° C. for 2 hours. The solution is filtered through filter paper and evacuated to dryness, again with the temperature of the solution not exceeding 37° C. The resultant product is dried under vacuum overnight to normally yield a white brittle solid. 
     The salt was then dried under vacuum over silica to constant weight (72 h). 
     Yield: 10.54 g. 
     Microanalysis: 
     
       
         
           
               
               
               
               
               
             
               
                   
                   
               
               
                   
                 C % Found 
                 H % Found 
                 N % Found 
                 B % Found 
               
               
                   
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
               
               
                   
                   
               
             
            
               
                   
                 52.47 
                 7.12 
                 15.25 
                 1.52 
               
               
                   
                 (56.65) 
                 (7.20) 
                 (14.01) 
                 (1.54) 
               
               
                   
                   
               
            
           
         
       
     
     Example 16 
     UV/Visible Spectra of Arginine Salt of TRI50C 
     UV/Visible spectra of the salt resulting from the procedure of Example 15 were recorded in distilled water at 20° C. from 190 nm to 400 nm. TRI50C and the salt gave λ max  at 210 and 258 nm. The weight of the dried salt was then measured for the purposes of calculating the extinction coefficient. The λ max  at 258 nm was used. The extinction coefficient was calculated using the formula:—
         A=εc1 where A is the absorbance   C is the concentration   I the path length of the UV cell   and ε is the extinction coefficient.   Extinction coefficient: 406.       

     Example 17 
     Aqueous Solubility of Arginine Salt of TRISDC 
     The salt used in this Example was made using a modification of the process described in Example 15. The modified process differs from that described in that 100 mg of TRI 50c was used as starting material, the product of the redissolution in water was dried by freeze drying and the filtration was carried out through a 0.2 μm filter. The salt is believed to contain about 85% of R,S,R isomer. 
     To determine maximum aqueous solubility 25 mg of the dried salt were shaken in water at 37° C., the sample filtered and the UV spectrum measured. The salt left a white residue of undissolved material. 
     Solubility when dissolved at 25 mg/ml: 14 mM (10 mg/ml). 
     Example 18 
     Preparation of Lysine Salt of TRI50C 
     Cbz-Phe-Pro-BoroMpg-OH obtained by the method of Example 4 (20.00 g, 38.1 mM) is dissolved in acetonitrile (200 ml) with stirring at room temperature. To this solution is added L-lysine as a 0.2M solution in distilled water (190 ml). The resultant clear solution is stirred for 2 hours at room temperature and then evacuated to dryness under vacuum with its temperature not exceeding 37° C. The resultant oil/tacky liquid is redissolved in 3 L distilled water with warming to 37° C. for 2 hours. The solution is filtered through filter paper and evacuated to dryness, again with the temperature of the solution not exceeding 37° C. The resultant product is dried under vacuum overnight to normally yield a white brittle solid. The product may be present as an oil or tacky solid (due to residual water), in which case it is then dissolved in ethyl acetate and evacuated to dryness to produce the product as a white solid. 
     The salt was then dried under vacuum over silica to constant weight (72 h). 
     Yield: 17.89. 
     Microanalysis: 
     
       
         
           
               
               
               
               
               
             
               
                   
                   
               
               
                   
                 C % Found 
                 H % Found 
                 N % Found 
                 B % Found 
               
               
                   
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
               
               
                   
                   
               
             
            
               
                   
                 57.03 
                 7.43 
                 10.50 
                 1.72 
               
               
                   
                 (59.11) 
                 (7.36) 
                 (10.44) 
                 (1.61) 
               
               
                   
                   
               
            
           
         
       
     
     Example 19 
     UV/Visible Spectra of Lysine Salt of TRI50C 
     UV/Visible spectra of the salt resulting from the procedure of Example 18 were recorded in distilled water at 20° C. from 190 nm to 400 nm. TRI50C and the salt gave λ max  at 210 and 258 nm. The weight of the dried salt was then measured for the purposes of calculating the extinction coefficient. The λ max  at 258 nm was used. The extinction coefficient was calculated using the formula:—
         A=εc1 where A is the absorbance   C is the concentration   I the path length of the UV cell   and ε is the extinction coefficient.   Extinction coefficient: 437.       

     Example 20 
     Aqueous Solubility of Lysine Salt of TRI50C 
     The salt used in this Example was made using a modification of the process described in Example 18. The modified process differs from that described in that 100 mg of TRI 50c was used as starting material, the product of the redissolution in water was dried by freeze drying and the filtration was carried out through a 0.2 μm filter. The salt is believed to contain about 85% of R,S,R isomer. 
     To determine maximum aqueous solubility 25 mg of the dried salt were shaken in water at 37° C., the sample filtered and the UV spectrum measured. The salt left a white residue of undissolved material. 
     Solubility when dissolved at 25 mg/ml: 13 mM (8.6 mg/ml). 
     Example 21 
     Preparation of N-Methyl-D-Glucamine Salt of TRI50C 
     Cbz-Phe-Pro-BoroMpg-OH obtained by the method of Example 4 (20.00 g, 38.1 mM) is dissolved in acetonitrile (200 ml) with stirring at room temperature. To this solution is added N-methyl-D-glucamine as a 0.2M solution in distilled water (190 ml). The resultant clear solution is stirred for 2 hours at room temperature and then evacuated to dryness under vacuum with its temperature not exceeding 37° C. The resultant oil/tacky liquid is redissolved in 500 ml distilled water with light warming for about 20 minutes. The solution is filtered through filer paper and evacuated to dryness, again with the temperature of the solution not exceeding 37° C., or freeze dried. The resultant product is dried under vacuum overnight to normally yield a white brittle solid. 
     The salt was then dried under vacuum over silica to constant weight (72 h). 
     Yield: 2131 g. 
     Microanalysis: 
     
       
         
           
               
               
               
               
               
             
               
                   
                   
               
               
                   
                 C % Found 
                 H % Found 
                 N % Found 
                 B % Found 
               
               
                   
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
                 (Calc.) 
               
               
                   
                   
               
             
            
               
                   
                 56.67 
                 7.28 
                 7.74 
                 1.63 
               
               
                   
                 (56.67) 
                 (7.41) 
                 (7.77) 
                 (1.50) 
               
               
                   
                   
               
            
           
         
       
     
     Example 22 
     UV/Visible Spectra of N-Methyl-D-Glucamine Salt of TRI50C 
     UV/Visible spectra of the salt resulting from the procedure of Example 21 were recorded in distilled water at 20° C. from 190 nm to 400 nm. TRI50C and the salt gave λ max  at 210 and 258 nm. The weight of the dried salt was then measured for the purposes of calculating the extinction coefficient. The λ max  at 258 nm was used. The extinction coefficient was calculated using the formula:—
         A=εc1 where A is the absorbance   C is the concentration   I the path length of the UV cell   and ε is the extinction coefficient.   Extinction coefficient: 433.       

     Example 23 
     Aqueous Solubility of N-Methyl-D-Glucamine Salt of TRI50C 
     The salt used in this Example was made using a modification of the process described in Example 21. The modified process differs from that described in that 100 mg of TRI 50c was used as starting material, the product of the redissolution in water was dried by freeze drying and the filtration was carried out through a 0.2 pm filter. The salt is believed to contain about 85% of R,S,R isomer. 
     To determine maximum aqueous solubility 25 mg of the dried salt were shaken in water at 37° C., the sample filtered and the UV spectrum measured. The salt was observed to fully dissolve. The salt was comparatively soluble and so was redissolved at 50 mg/ml in the same manner previously described. 
     Solubility when dissolved at 25 mg/ml: 35 mM (25 mg/ml). 
     Solubility when dissolved at 50 mg/ml: 70 mM (50 mg/ml). 
     Example 24 
     Alternative Preparation of Arginine Salt of TRI50C 
     The arginine salt is formed simply by adding a slight molar excess of L-arglnlne to a solution of 0.2-0.3 mmol of TRI 50c in 10 ml of ethyl acetate. The solvent is evaporated after one hour, and the residue is triturated twice with hexane to remove excess arginine. 
     Example 25 
     Solubility of TRI50C 
     The UV/visible spectra of TRI 50c resulting from the procedure of Example 5 and its solubility were obtained as described above in relation to the salts. The solubility of TRI 50c when dissolved at 50 mg/ml was 8 mM (4 mg/ml). 
       13 C NMR 75 MHz δc(CD 3 C(O)CD 3 ) 206.56, 138.30, 130.76, 129.64, 129.31, 129.19, 129.09, 128,20, 128.04, 74.23, 73.55, 67.78, 58.76, 56.37, 56.03, 48.38, 47.87, 39.00, 25.42, 25.29. FTIR (KBr disc)  vmax  (cm −1 ) 3331.3, 3031.4, 2935.3, 2876.9, 2341.9, 1956.1, 1711.6, 1639.9, 1534.3, 1498.1, 1453,0, 1255.3, 1115.3, 1084.6, 1027.6, 917.3, 748.9, 699.6, 594.9, 504.5, 467.8. 
     Example 26 
     Analysis of Sodium and Zinc Salts of (R,S,R) TRI 50C 
     The following salts were prepared using a boronate:metal stoichiometry of n:1, where n is the valency of the metal, using (R,S,R) TRI 50c of higher chiral purity than that used to prepare the salt described in Example 10. 
     A. Sodium Salt 
       
     
       
         
           
               
               
             
               
                   
                   
               
             
            
               
                   
                 Analytical data 
               
               
                   
                   
               
               
                   
                 HPLC or LC/MS: HPLC betabasic C18 Column, 
               
               
                   
                 CH 3 CN, Water 
               
               
                   
                 Estimated Purity: &gt;95% by UV (λ215 nm) 
               
               
                   
                   
               
            
           
           
               
            
               
                 Micro analysis: 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 Calcd. 
                 Found. 
               
               
                   
                   
               
               
                   
                 C: 
                   
                 59.24 
                 59.93 
               
               
                   
                 H: 
                   
                 6.44 
                 6.47 
               
               
                   
                 N: 
                   
                 7.67 
                 7.31 
               
               
                   
                 Other: 
                 B: 
                 1.98 
               
               
                   
                   
                 Na: 
                 4.20 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 Physical Properties 
               
               
                   
                   
               
               
                   
                 Form: Amorphous solid 
               
               
                   
                 Colour: White 
               
               
                   
                 Melting Point: N/A 
               
               
                   
                 Solubility: Soluble in aqueous media 
               
               
                   
                 ca~50 mg/ml 
               
               
                   
                 M W : 547.40 
               
               
                   
                 1.91 
               
               
                   
                 3.81 
               
               
                   
                   
               
            
           
         
       
     
     B. Zinc Salt 
       
     
       
         
           
               
               
             
               
                   
                   
               
             
            
               
                   
                 Analytical data 
               
               
                   
                   
               
               
                   
                 HPLC or LC/MS: HPLC betabasic C18 Column, 
               
               
                   
                 CH 3 CN, Water 
               
               
                   
                 Estimated Purity: &gt;95% by UV (λ, 215 nm) 
               
               
                   
                   
               
            
           
           
               
            
               
                 Micro analysis: 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 Calcd. 
                 Found. 
               
               
                   
                   
               
               
                   
                 C: 
                   
                 58.21 
                 56.20 
               
               
                   
                 H: 
                   
                 6.33 
                 6.33 
               
               
                   
                 N: 
                   
                 7.54 
                 7.18 
               
               
                   
                 Other: 
                 B: 
                 1.94 
                 1.84 
               
               
                   
                   
                 Zn: 
                 5.87 
                 7.26 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 Physical Properties 
               
               
                   
                   
               
               
                   
                 Form: Amorphous solid 
               
               
                   
                 Colour: White 
               
               
                   
                 Melting Point: N/A 
               
               
                   
                 Solubility: Soluble in aqueous media 
               
               
                   
                 ca~2 mg/ml 
               
               
                   
                 M W : 1114.18 
               
               
                   
                   
               
               
                   
                 Notes: 
               
               
                   
                 The trigonal formula of the acid boronate is used in the calculated microanalyses. 
               
               
                   
                 It is believed that a lower sodium salt solubility is reported In example 11 because the salt tested in example 11 had lower chiral purity. 
               
            
           
         
       
     
     Conclusion 
     The sodium and zinc salts have all been prepared with a stoichiometry of, respectively, one metal ion to one molecule of TRI 50c and one metal ion to two molecules of TRI 50c. The value found for the sodium salt is dose to and thus consistent with that calculated for this 1:1 stoichiometry. For the zinc salt an excess of zinc was found; nonetheless, the zinc salt comprises a significant proportion of acid boronate. 
     Example 27 
     Stability 
     An assay of TRI 50c and its sodium and lysine salts before and after drying. 
     Method 
     TRI 50c and its Na, Ca and Lys salts were weighed into HPLC vials and stored in a desiccator over phosphorus pentoxide for 1 week. For sample analysis, 5 mg of dried and non-dried material was weighed in a 5 mL volumetric flask and dissolved in 1 mL acetonitrile and filled up with water to 5 mL. 
     The compounds were investigated by HPLC. For the impurity profiles, an HPLC peak area percentage was calculated. The results are shown in Table 1. 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 1 
               
               
                   
                   
               
               
                   
                   
                 Amount 
                   
               
               
                   
                 Compound 
                 [μg/mL] 
                 Purity (% area) 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 TRI 50c dry 
                 1000.0 
                 82.00 
               
               
                   
                 TRI 50c non-dried 
                 947.3 
                 85.54 
               
               
                   
                 TRI 50c Na salt dry 
                 1024 
                 98.81 
               
               
                   
                 TRI 50c Na salt non-dried 
                 1005.8 
                 98.61 
               
               
                   
                 TRI 50c Lys salt dry 
                 813.3 
                 90.17 
               
               
                   
                 TRI 50c Lys salt non-dried 
                 809.8 
                 92.25 
               
               
                   
                   
               
            
           
         
       
     
     The purity of the acid was lowered by the drying process but the purity of the salts was less affected; the purity of the sodium salt was not significantly reduced. Large differences in response factors will reduce the actual impurity levels, however. 
     This example indicates that the salts of the disclosure, particularly the metal salts, e.g. alkali metal salts, are more stable than the adds, notably TRI 50c. 
     Example 28 
     Stability 
     This Example compares the stability of TRI 50c and TRI 50c lysine salt when filled into enteric-coated hard gelatin capsules. 
     1. Tabulated Results 
       
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                   
                   
                 Climatic 
                 Purity 
                 Purity 
               
               
                   
                   
                 conditions 
                 (HPLC % 
                 (HPLC % Area) 3   
               
               
                 Compound 
                 Packing 
                 1.5 month°) 
                 Area) TO 
                 T1 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 TRI50c 
                 capsules 
                 25° C./60% 
                 99 
                 73 9 
               
               
                   
                 in blister 
                 r.h. 4   
               
               
                 TRI50c 
                 capsules 
                 40° C./75% 
                 99 
                 73.9 
               
               
                   
                 in blister 
                 r.h 
               
               
                 TRI50c 
                 capsules&#39; 
                 40° C. h75% 
                 99 
                 75.3 
               
               
                   
                   
                 r.h 
               
               
                 TRI50c 
                 capsules 
                 25° C./60% 
                 90.2 2)   
                 90.5 
               
               
                 Lysine Salt 
                 in blister 
                 r.h. 
               
               
                 TRI50c 
                 capsules 
                 40° C./75% 
                 90.2 2   
                 91.8 
               
               
                 Lysine Salt 
                 in blister 
                 r.h 
               
               
                 TRI50c 
                 capsules 1   
                 40° C./75% 
                 90.2 2   
                 90.6 
               
               
                 Lysine Salt 
                   
                 r.h 
               
               
                   
               
               
                 Notes: 
               
               
                 °)1.5 month storage at given conditions, samples were then stored at room temperature until analytical testing. 
               
               
                   1 capsules stored at the respective climatic conditions without blister. 
               
               
                   2 purity of the batch before storage. 
               
               
                   3 purity of the stored batch (capsules were poured out, the contents of the capsules were then analyzed). 
               
               
                   4 r.h. = relative humidity 
               
            
           
         
       
     
     Conclusion 
     There was no significant difference in the purities of the lysine salt at T 0  and T 1 . 
     2. Analytical Procedure 
     2.1 Sample Preparation 
     2.1.1 Assay of TRI 50c and Salts 
     TRI 50c-standard (free acid) was stored in a desiccator over phosphorus pentoxide for 2 days for drying. Afterwards, the reference standard was weighed in a volumetric flask and dissolved in a mixture of acetonitrile and water (25/75 v/v %). Aliquots of the resulting solution (ST 1A) were diluted successively with water as shown in the dilution scheme of table 4. 
     Stock—and Calibration solutions of Tri 50c 
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                   
               
             
            
               
                   
                 Net 
                   
                   
                   
                   
                   
                   
               
               
                   
                 weight 
                 Purity 
                   
                 Dissolved in 
                   
                 Conc. 
                 Calibr.- 
               
               
                   
                 mg 
                 % 
                 Salt- 
                 ml 
                 Solvent 
                 [μg/ml] 
                 [μg/ml] 
               
               
                   
               
               
                 ST 1. A 
                 40.8 
                 98.23 
                 1 
                 10 
                 ACN/water 
                 4007.8 
                 C4000 
               
               
                   
                   
                   
                   
                   
                 25/75 (v/v %) 
               
               
                   
               
               
                   
                 ml 
                 ST 
                 [μg/ml] 
                 ad ml 
                 Solvent 
                 [μg/ml] 
               
               
                   
               
               
                 ST 2 A 
                 5 
                 1 A 
                 4007.8 
                 10 
                 water 
                 2003.9 
                 C2000 
               
               
                 ST 3 A 
                 5 
                 2 A 
                 2003.9 
                 10 
                 water 
                 1001.9 
                 C1000 
               
               
                 ST 4 A 
                 5 
                 3 A 
                 1001.9 
                 10 
                 water 
                 501.0 
                 C500 
               
               
                 ST 5 A 
                 5 
                 4 A 
                 500.9 
                 10 
                 water 
                 250.5 
                 C250 
               
               
                 ST 6 A 
                 1 
                 3 A 
                 1001.9 
                 10 
                 water 
                 100.2 
                 C100 
               
               
                 ST 7 A 
                 1 
                 6 A 
                 100.2 
                 10 
                 water 
                 10.0 
                 C10 
               
               
                   
               
            
           
         
       
     
     2.1.2 Impurity Profile of the Stored Capsules 
     The stored capsules of every batch at corresponding climatic condition were removed and 10 mg of the content was weighed in a 10 ml volumetric flask and dissolved in 10 ml of a mixture of acetonitrile/water (25/75 v/v/0). These solutions were injected for impurity profile analysis and for quantification respectively. 
     3. Data Evaluation 
     The quantitative evaluation and the impurity profile analysis were performed using an HPLC-PDA method. The processing wavelength was set as 258 nm. 
     4. Analytical Parameters 
     4.1 Equipment and Software 
       
     
       
         
           
               
               
             
               
                   
               
             
            
               
                 Autosampler 
                 Waters Alliance 2795 
               
               
                 Pump 
                 Waters Alliance 2795 
               
               
                 Column oven 
                 Waters Alliance 2795 
               
               
                 Detection 
                 Waters 996 diode array, extracted wavelength 258 nm 
               
               
                 Software version 
                 Waters Millennium Release 4.0 
               
               
                   
               
            
           
         
       
     
     4.2 Stationary Phase 
       
     
       
         
           
               
               
             
               
                   
               
             
            
               
                 Analytical Column ID 
                 S71 
               
               
                 Material 
                 X-Terra ™ MS C 18 , 5 μm 
               
               
                 Supplier 
                 Waters, Eschborn, Germany 
               
               
                 Dimensions 
                 150 mm × 2.1 mm (length, internal diameter) 
               
               
                   
               
            
           
         
       
     
     4.3 Mobile Phase 
       
     
       
         
           
               
            
               
                   
               
               
                 Aqueous phase: A: 0.1% HCOOH in water 
               
               
                 Organic phase: C: ACN 
               
               
                 Gradient conditions: 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Time 
                 Flow 
                 % A 
                 % C 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 0.00 
                 0.5 
                 90 
                 10 
               
               
                   
                 27.0 
                 0.5 
                 10 
                 90 
               
               
                   
                 27.1 
                 0.5 
                 90 
                 10 
               
               
                   
                 30.0 
                 0.5 
                 90 
                 10 
               
               
                   
                   
               
            
           
         
       
     
     Example 29 
     TRI50B Inhibition of Platelet Procoagulant Activity 
     Platelet pro-coagulant activity may be observed as the increase, in rate of activation of prothrombin by factor Xa in the presence of factor Va upon the addition of platelets pretreated with thrombin, caused by thrombin alone, collagen alone or a mixture of thrombin and collagen. This property is due to an increase in anionic phospholipid on the surface of the platelet with concomitant release of microvesicie from the surface. This is an essential physiological reaction and people whose platelets have reduced ability to generate procoagulant activity (Scott syndrome) show an increased tendency for bleeding. 
     Method: 
     Washed platelets were treated with either 1.15 nM thrombin, 23 μg/ml collagen or a mixture of both at the same concentration at 37° C. TRI 50b was added either for 1 minute prior to the addition of activator or immediately after the incubation with activator. Platelet procoagulant activity was determined as described previously (Goodwin C A et al,  Biochem J.  1995 8, 308: 15-21). 
     TRI 50b proved to be a potent Inhibitor of platelet procoagulant activity with ICSO&#39;s as summarised below: 
     Table 2: Influence of TRI 50b on the induction of platelet procoagulant activity by various agonists: 
     
       
         
           
               
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
               
                   
                   
                 IC50 plus pre- 
                 IC50 without 
               
               
                   
                 Fold acceleration 
                 incubation 
                 incubation 
               
               
                 Agonist 
                 without TRI 50b 
                 (nM) 
                 (nM) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 Thrombin 
                 30 
                 8 
                 3000 
               
               
                 Collagen 
                 45 
                 200 
                 300 
               
               
                 Thrombin/Collagen 
                 11.0 
                 3 
                 80 
               
               
                   
               
            
           
         
       
     
     Table 2 records, for example, that when platelets were treated with thrombin they caused a 30-fold acceleration of the rate of activation of prothrombin in comparison with control platelets. Treatment with TRI 50 reduced such acceleration by half at the various TRI 50 concentration levels given. The significant potency of TRI 50 is evidenced by the fact that the IC 50  values are in the nanomolar range. 
     TRI 50b does not have an effect on ADP, collagen or epinephrine induced aggregation of washed platelets. 
     Example 30 
     Rabbit Extracorporeal Shunt Model 
     Introduction 
     The technique describes an animal model in which a platelet rich thrombus is produced. The activity of TRI 50b and heparin are compared. 
     The carotid artery and jugular vein of anaesthetised rabbits were used to create an extracorporeal circuit containing a suspended foreign surface (silk thread). Thrombus deposition is initiated by creation of high sheer stress turbulent arterial blood flow, platelet activation, followed by coagulation in the presence of thrombogenic surfaces. Histopathological studies have shown that the thrombus is platelet rich. 
     Materials and Methods 
     Animals: 
     NZW rabbits (males 2.5-3.5 kg) were used. The animals were allowed food and water up to the Induction of anaesthesia. 
     Anaesthesia: 
     Animals were premeditated with fontanel/fluanisone (Hypnorm) 0.15 ml total by intramuscular injection. General anaesthesia was induced with methohexitone (10 mg/ml) to effect, followed by endotracheal intubation. Anaesthesia was maintained with isoflurane (1-2.0%) carried in oxygen/nitrous oxide. 
     Surgical Preparation: 
     The animals were placed in dorsal recumbency and the ventral cervical region prepared for surgery. The left carotid artery and right jugular vein were exposed. The artery was cannulated with a large Portex® catheter (yellow gauge), cut to a suitable length. The vein was cannulated with a Silastic® catheter. The shunt comprised of a 5 cm length of ‘auto analyser’ line (purple/white gauge). Joins to the shunt on the arterial side were made with intermediate size Silastic® tubing. The shunt was filled with saline before exposure to the circulation, The right femoral artery was cannulated for the measurement of blood pressure. 
     Thread Preparation and Insertion: 
     The central section of the shunt contained a thread 3 centimetres in length. This consisted of 000 gauge Gutterman sewing silk so as to give four strands with a single knot at the end. (The knot section was outside the shunt). 
     Blood Flow 
     Blood flow velocity was determined by use of ‘Doppler’ probes (Crystal Biotech). A silastic probe was positioned over the carotid artery at the point of insertion of the arterial catheter, Flow was recorded on a chart recorder using heat sensitive paper. 
     Results 
       
     
       
         
           
               
               
               
               
             
               
                 TABLE 3 
               
               
                   
               
               
                   
                   
                 THROMBUS 
                 ANTITHROM- 
               
               
                 TREAT- 
                   
                 WEIGHT 
                 BOTIC 
               
               
                 MENT 
                 DOSE 
                 AFTER 20 minute run 
                 ACTIVITY 
               
               
                   
               
             
            
               
                 Control 
                 N/A 
                 22.4 ± 2.2 mg (n = 5) 
                   
               
               
                 TRI 50b 
                  10 mg/kg iv 
                 9.78 ± 1.9 mg (n = 5) 
                 Active 
               
               
                   
                  3.0 mg/kg iv 
                 15.3 ± 2.2 mg (n = 5) 
                 Active 
               
               
                 HEPARIN 
                 100 u/kg iv 
                 22.9 ± 1.65 mg (n = 4) 
                 Inactive 
               
               
                   
                 300 u/kg iv 
                 10.5 ± 1.4 mg (n = 4) 
                 Active (Severe 
               
               
                   
                   
                   
                 bleeding) 
               
               
                   
               
            
           
         
       
     
     Discussion 
     Table 3 shows that, under high arterial shear conditions, a TRI 50b dose of 3 mg/kg to 10 mg/kg iv significantly inhibits thrombus formation without bleeding, whereas a heparin dose within the normal clinical range for treating venous thrombosis (100 u/kg iv heparin) was ineffective. The higher dose of heparin, though active, caused severe bleeding. These results, which show TRI 50b effectively inhibiting arterial thrombosis without causing bleeding, are consistent with TRI 50b inhibiting platelet procoagulant activity. In contrast, the thrombin inhibitor heparin, when administered at an approximately equi-effective dose (in terms of inhibition of arterial thrombosis), produced the severe bleeding normal when thrombin inhibitors are used to treat arterial thrombosis. 
     Example 31 
     Comparison of Bleeding Times 
     The aim of the study was to compare the bleeding times of heparin with TRI 50b in a suitable model. It is accepted that heparin is a poor inhibitor of platelet procoagulant activity ( J. Biol. Chem.  1978 Oct. 10; 253(19):6908-16; Miletich J P, Jackson C M, Majerus PW1 : J. Clin. Invest.  1983 May; 71(5): 1383-91). 
     Bleeding times were determined in a rat tail bleeding model following intravenous administration of heparin and TRI 50b. The doses employed were chosen on the basis of their efficacy in the rat Wessler and dynamic models and were as follows: 
     
       
         
           
               
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 TRI 50b: 
                 5 and 10 
                 mg/kg 
               
               
                   
                 Heparin: 
                 100 
                 units/kg 
               
               
                   
                   
               
            
           
         
       
     
     Materials and Methods 
     Anaesthesia 
     Rats were anaesthetised with sodium pentabarbitone at 60 mg/kg (2.0 ml/kg of 30 mg/ml solution by ip, injection). Supplemental anaesthetic was given ip, as required. 
     Surgical Preparation 
     A jugular vein was cannulated for the administration of test compound. The trachea was also cannulated with a suitable cannula and the animals allowed to breathe ‘room air’ spontaneously. 
     Compound Administration 
     These were given in the appropriate vehicle at 1.0 ml/kg intravenously. Heparin was administered in saline, whilst TRI 50b was dissolved in ethanol, and then the resultant solution added to water for injection (1 part ethanol to 5 parts water). 
     Technique 
     Two minutes following compound administration the distal 2 mm of the animal&#39;s tail was sectioned with a new scalpel blade and the tail immersed in warm saline (37° C.) contained in a standard ‘universal’ container, so that the blood stream was dearly visible. The bleeding time recording was started immediately following transection until the cessation of blood now from the tip of the tail. A period of 30 seconds was allowed after the blood flow from the tail had stopped to ensure that bleeding did not re-commence, if bleeding did start again the recording time was continued for up to a maximum of 45 minutes. 
     Results 
     Table 4 gives a summary of the bleeding results and shows the increases above base line values. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 4 
               
               
                   
                   
               
               
                   
                   
                 Bleeding time min 
               
               
                   
                 Treatment 
                 (±SEM + ) 
               
               
                   
                   
               
             
            
               
                   
                 Saline 
                  5.1 ± 0.6 
               
               
                   
                 Heparin 100 u/kg iv 
                 &gt;40* 
               
               
                   
                 TRI 50b 5 mg/kg iv 
                 11.3 * 1.2 
               
               
                   
                 TRI 50b 10 mg/kg iv 
                 30.4 ± 5.2 
               
               
                   
                   
               
               
                   
                 *Severe bleeding in all animals, with no cessation after 40 minutes. 
               
               
                   
                   + SEM = standard error of the mean 
               
            
           
         
       
     
     Discussion 
     The results show that TRI 50b was superior to heparin (produced less bleeding) at all doses. It should be noted that when 100 u/kg heparin is compared with 5 mg/kg TRI 50b, heparin-treated animals bled more extensively than those receiving TRI 50b; it was previously established (Example 25) that heparin at a dose of 100 u/kg is a less effective inhibitor of arterial thrombosis than TRI 50b at a dose of 3.0 mg/kg. Heparin is primarily a thrombin inhibitor and a poor inhibitor of platelet procoagulant activity; the results are therefore consistent with TRI 50b exerting anti-coagulant activity by inhibition of platelet coagulant activity in addition to thrombin inhibiting activity. 
     Example 32 
     TRI5DB as a Prodrug for TRI 50C: Pharmacokinefics and Absorption 
     Materials and Methods 
     Animals 
     Rats, body weight circa 250-300 g were used. The animals were fasted only on the day of use for the iv stage. Animals were fasted on the night prior to study for the oral and intraduodenal studies, water was allowed up to the time of anaesthesia. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 oral phase 
               
            
           
           
               
               
               
               
            
               
                   
                 Treatment 
                 Dose mg/kg po 
                 n 
               
               
                   
                   
               
               
                   
                 TRI 50b 
                 20 mg/kg 
                 2 
               
               
                   
                 TRI 50c 
                 20 mg/kg 
                 2 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 intraduodenal phase 
               
            
           
           
               
               
               
               
            
               
                   
                 Treatment 
                 Dose mg/kg po 
                 n 
               
               
                   
                   
               
               
                   
                 TRI 50b 
                 20 mg/kg 
                 3 
               
               
                   
                 TRI 50c 
                 20 mg/kg 
                 3 
               
               
                   
                   
               
            
           
         
       
     
     Dose 
     Formulation (TRI 50b/TRI 50c) 
     These were dosed in a formulation prepared as follows: 48 mg/ml of TRI 50b is dissolved in ethanol: PEG 300 (2:3 vol:vol). Just before administration, 5 volumes of this solution is mixed with 3 volumes of 5% kollidon 17 8F. 
     1) Both compounds were dosed by oral gavage, or directly into the duodenum, at 20 mg/kg. 
     The compounds were dosed in a PEG/ethanol/kollidon formulation which was prepared immediately before, as described immediately under the heading “Dose”: Stock 15.0 mg/ml. This was dosed at 1.33 ml/kg (equivalent to 30 mg/kg). 
     Methods 
     Oral Gavage 
     Rats were dosed at 20 mg/kg. Approximately 30 minutes following dosing the rats were anaesthetised. 
     Intraduodenal Administration 
     The compounds were instilled directly into the duodenum after anaesthesia and surgical procedures had been completed. 
     Blood Sampling 
     Oral Phase 
     Blood (0.81 ml) was taken from the carotid cannula into (0.09 ml) of 3,8% w/v tri sodium citrate following anaesthesia and surgery. The first samples were taken one-hour post dose. Then at, 1.5, 2, 4 hours post dose. 
     Intraduodenal Phase 
     Blood samples were taken: Pre dose, then at 0.25, 0.5, 0.75, 1.0, 2, 3 and 4 hours post dosing. 
     Plasma 
     This was obtained by centrifugation (3000 RPM for 10 min) and stored at −20° C. prior to analysis. 
     Results 
     Pharmacokinetic Analysis 
       FIG. 1 : oral phase clearance and kinetics following dosing with TRI 50b or its free acid (TRI 50c). 
       FIG. 2 : oral phase clearance and kinetics following intraduodenal dosing with TRI 50b or its free acid (TRI 50c). 
     Conclusion 
     When given by the intraduodenal route TRI 50b achieved a higher bioavaiiability (peak plasma concentration) than the free add. The data are consistent with TRI 50b being rapidly hydrolysed in plasma to TRI 50c and with TRI 50c being the active principle. 
     The results of examples 29 to 32 indicate that administration of TRI 50c as a salt will provide a way to treat arterial thrombosis and/or venous thrombosis. 
     Example 33 
     Preparation of Calcium Salt of TRI50C (TGN 167) 
     1.5 kg (2.5 mole) TRI50d from Example 2 is dissolved in 10.5 L dichloromethane. 11 L 2% hydrochloric acid is added and the mixture is stirred for at most 30 minutes (optimally about 20 minutes) at room temperature. After stirring the layers are allowed to settle and separated. The aqueous layer is given a rewashed twice with 2.2 L dichloromethane. The combined organic layers are washed with a solution of 625 g ammonium chloride in 2.25 L water. The organic phase is dried over magnesium sulfate, filtered and the filtrate evaporated to dryness. An assay of the free boronic acid is performed and the amounts of the solvents and base for conversion of the acid to the salt are calculated. If 2.5 mol of the free acid is obtained, the evaporation residue is dissolved In 5 L acetonitrile followed by addition of a suspension of 93 g (1.25 mole) calcium hydroxide In 1 L water. The solution is stirred for two hours at ambient temperature (e.g. 15-30° C., optimally room temperature) and then evaporated under vacuum (of ca. 10 mmHg) at a temperature initially of about 10° C. and then increasing to a limit of about 35° C. The evaporation residue is repeatedly dissolved in 3.5 L fresh acetonitrile and evaporated to dryness to remove traces of water. If the evaporation residue is dry, it is dissolved in 6 L tetrahydrofuran and slowly added to a mixture of 32 L n-heptane and 32 L diethylether. The addition is performed slowly enough to avoid lumping or sticking of the product and is carried out over a period of not less than 30 minutes. The precipitated product is filtered off, washed with n-heptane and dried under vacuum (of ca. 10 mmHg) at a temperature below 35° C. until dry. 
     Yield: 0.98 kg (70%) Tri50c calcium salt. 
     The procedures of Examples 1 to 3 and 33 may be scaled up and, if operated carefully, will produce highly pure salts. In the diethanolamine precipitation step it is important to use 1.25 equivalents of diethanolamine per equivalent of (R,S,R) TRI 50b. In the hydrolysis of the diethanolamine ester, it is Important to avoid excessively long contact with the aqueous acid. Likewise the TRI 50b should be synthesised via the Grignard reaction to Z-DIPIN A. 
     Example 34 
     Human Clinical Studies 
     In human clinical volunteer studies with doses of up to 2.5 mg/kg i.v. (dosages which significantly prolong the thrombin clotting time), TRI 50b had no effect on Simplate bleeding time (i.e. bleeding time measured using a Simplate® bleeding time device). 
     It will be appreciated from the foregoing that the disclosure provides boronic acid salts useful for pharmaceutical purposes and which feature one or more of the following attributes: (1) improved amount of oral bioavailability; (2) improved consistency of oral bioavailability; (3) improved stability; and (4), in any event, not suggested by the prior art. 
     The selection of active ingredient for a pharmaceutical composition is a complex task, which requires consideration not only of biological properties (including bioavailability) but also of physicochemical properties desirable for processing, formulation and storage. Bioavallability itself is dependent on various factors, often including in vivo stability, solvation properties and absorption properties, each in turn potentially dependent on multiple physical, chemical and/or biological behaviours. 
     Advantageously, at least preferred products of the disclosure have adequate absorpi bioavaliability. For commercial utility, a product having less good solubility may be sell virtue of a superior overall combination of properties. 
     The present disclosure includes the subject matter of the following paragraphs: 
     1. A pharmaceutically acceptable base addition salt of a boronic acid of formula (I): 
     
       
         
         
             
             
         
       
     
     wherein 
     Y comprises a hydrophobic moiety which, together with the aminoboronic acid residue —NHCH(R 9 )—B(OH) 2 , has affinity for the substrate binding site of thrombin; and 
     R 9  is a straight chain alkyl group interrupted by one or more ether linkages and in which the total number of oxygen and carbon atoms is 3, 4, 5 or 6 or R 9  is —(CH 2 ) m —W where m is 2, 3, 4 or 5 and W is —OH or halogen (F, Cl, Br or I). 
     2. A salt of paragraph 1 wherein R 9  is an alkoxyalkyl group. 
     3. A salt of paragraph 1 or paragraph 2 wherein YCO— comprises an amino acid which binds to the S2 subsite of thrombin, the amino acid being N-terminally linked to a moiety which binds the S3 subsite of thrombin. 
     4. A salt of paragraph 1 or paragraph 2 wherein Y is an optionally N-terminally protected dipeptide which binds to the S3 and S2 binding sites of thrombin and the peptide linkages in the acid are optionally and independently N-substituted by a C 1 -C 13  hydrocarbyl optionally containing in-chain or in-ring nitrogen, oxygen or sulfur and optionally substituted by a substituent selected from halo, hydroxy and trifluoromethyl. 
     5. A salt of paragraph 4 wherein said dipeptide is N-terminally protected. 
     6. A salt of paragraph 4 or paragraph 5 wherein all the peptide linkages in the acid are unsubstituted. 
     7. A salt of any of paragraphs 1 to 6 wherein S3-binding amino acid residue is of R configuration, the S2-binding residue is of S configuration, and the fragment —NHCH(R 9 )—B(OH) is of R configuration. 
     8. A salt of any of paragraphs 1 to 7 wherein the boronic acid has a Ki for thrombin of about 100 nM or less. 
     9. A salt of paragraph 8 wherein the boronic add has a Ki for thrombin of about 20 nM or less. 
     10. A pharmaceutically acceptable base addition salt of a boronic acid of formula (II): 
     
       
         
         
             
             
         
       
     
     where: 
     X is H (to form NH 2 ) or an amino-protecting group; 
     aa 1  is an amino acid having a hydrocarbyl side chain containing no more than 20 carbon atoms and comprising at least one cyclic group having up to 13 carbon atoms; 
     aa 2  is an amino acid having from 4 to 6 ring members; 
     R 1  is a group of the formula —(CH 2 ) s -Z, where s is 2, 3 or 4 and Z is —OH, —OMe, —OEt or halogen (F, Cl, Br or I). 
     11. A salt of paragraph 10 wherein aa 1  is selected from Phe, Dpa and wholly or partially hydrogenated analogues thereof. 
     12. A salt of paragraph 10 wherein aa 1  is selected from Dpa, Phe, Dcha and Cha. 
     13. A salt of any of paragraphs 10 to 12 wherein aa 1  is of R-configuration. 
     14. A salt of paragraph 10 wherein aa 1  is (R)-Phe or (R)-Dpa. 
     15. A salt of paragraph 10 wherein aa 1  is (R)-Phe. 
     16. A salt of any of paragraphs 10 to 15 wherein aa 2  is a residue of an imino add of formula (IV) 
     
       
         
         
             
             
         
       
     
     where R 11  is —CH 2 —, —CH 2 —CH 2 —, —S—CH 2 —, —S—C(CH 3 ) 2 — or —CH 2 —CH 2 —CH 2 —, which group, when the ring is 5- or 6-membered, is optionally substituted at one or more —CH 2 — groups by from 1 to 3 C 1 -C 3  alkyl groups. 
     17. A salt of paragraph 16 wherein aa 2  is of S-configuration. 
     18. A salt of paragraph 16 wherein aa 2  is an (S)-proline residue. 
     19. A salt of paragraph 10, wherein aa 1 -aa 2  is (R)-Phe-(S)-Pro. 
     20. A salt of any of paragraphs 10 to 19 wherein the fragment —NH—CH(R 1 )— B(OH) 2  is of R configuration. 
     21. A salt of any of paragraphs 10 to 20 wherein R 1  is 2-bromoethyl, 2-chloroethyl, 2-methoxyethyl, 3-bromopropyl, 3-chloropropyl or 3-methoxypropyl. 
     22. A salt of any of paragraphs 10 to 20 wherein R 1  is 3-methoxypropyl. 
     23. A salt of paragraph 10 which is a salt of a compound of formula (VIII): 
       X—(R)-Phe-(S)-Pro-(R)-Mpg-B(OH) 2   (VIII). 
     24. A salt of any of paragraphs 10 to 23 where X is R 6 —(CH 2 )p-C(O)—, R 6 —(CH 2 )p-S(O) 2 —, R 6 —(CH 2 )p NH—C(O)— or R 6 —(CH 2 )p-O—C(O)— wherein p is 0, 1, 2, 3, 4, 5 or 6 and R 6  is H or a 5 to 13-membered cyclic group optionally substituted by 1, 2 or 3 substituents selected from halogen, amino, nitro, hydroxy, a C 5 -C 6  cyclic group, C 1 -C 4  alkyl and C 1 -C 4  alkyl containing, and/or linked to the cyclic group through, an in-chain O, the aforesaid alkyl groups optionally being substituted by a substituent selected from halogen, amino, nitro, hydroxy and a C 5 -C 6  cyclic group. 
     25. A salt of paragraph 24 wherein said 5 to 13-membered cyclic group is aromatic or heteroaromatic. 
     26. A salt of paragraph 25 wherein said 5 to 13-membered cyclic group is phenyl or a 6-membered heteroaromatic group. 
     27. A salt of any of paragraphs 24 to 26 wherein X is R 6 —(CH 2 )p C(O)— or R 6 —(CH 2 )p-O—C(O)— and p is 0 or 1. 
     28. A salt of any of paragraphs 10 to 22 wherein X is benzyloxycarbonyl. 
     29. A salt of any of paragraphs 1 to 28 which does not comprise a choline or ammonium salt. 
     30. A salt of any of paragraphs 1 to 29 which comprises boronate ions derived from the boronic acid and monovalent counter-ions. 
     31. A salt of any of paragraphs 1 to 29 which is a salt of the peptide boronic acid with an alkali metal or a strongly basic organic nitrogen-containing compound. 
     32. A salt of paragraph 21 wherein the strongly basic organic nitrogen compound has a pKb of about 7 or more, e.g. about 7.5 or more. 
     33. A salt of paragraph 31 or paragraph 32 wherein the strongly basic organic nitrogen-containing compound is a guanidine, a guanidine analogue or an amine. 
     34. A salt of any of paragraphs 1 to 33 which is a salt of the boronic acid with a metal. 
     35. A salt of any of paragraphs 1 to 28 which is a salt of the boronic acid with an alkali metal, an aminosugar, a guanidine or an amine of formula (XI): 
     
       
         
         
             
             
         
       
     
     where n is from 1 to 6, R 2  is H, carboxylate or derivatised carboxylate, R 3  is H, C 1 -C 4  alkyl or a residue of a natural or unnatural amino acid. 
     36. A salt of any of paragraphs 1 to 28 which is a salt of the boronic acid with a guanidine or with an amine of formula (IX): 
     
       
         
         
             
             
         
       
     
     where n is from 1 to 6, R 2  is H, carboxylate or derivatised carboxylate, R 3  is H, C 1 -C 4  alkyl or a residue of a natural or unnatural amino acid. 
     37. A salt of paragraph 36 which is a guanidine salt of the boronic acid. 
     38. A salt of paragraph 37 which is a salt of the boronlc acid with L-arginine or an L-arginine analogue. 
     39. A salt of paragraph 38 wherein the L-arginine analogue is D-arginine, or the D- or L-isomers of homoarginine, agmatine [(4-aminobutyl) guanidine], NG-nitro-L-arginine methyl ester, or a 2-amino pyrimidines. 
     40. A salt of paragraph 37 which is a salt of the boronic acid with a guanidine of formula (VII) 
     
       
         
         
             
             
         
       
     
     where n is from 1 to 6, R 2  is H, carboxylate or derivatised carboxylate, R 3  is H, C 1 -C 4  alkyl or a residue of a natural or unnatural amino acid. 
     41. A salt of paragraph 40 where the derivatised carboxylate forms a C 1 -C 4  alkyl ester or amide. 
     42. A salt of paragraph 40 or paragraph 41 wherein the compound of formula (VII) Is of L-configuration. 
     43. A salt of paragraph 37 which is an L-arginine salt of the peptide boronic acid. 
     44. A salt of paragraph 36 which is a salt of the boronic acid with an amine of formula (IX). 
     45. A salt of paragraph 41 where the derivatised carboxylate forms a C 1 -C 4  alkyl ester or amide. 
     46. A salt of paragraph 44 or paragraph 45 wherein the amine of formula (1X) is of L-configuration. 
     47. A salt of paragraph 44 which is an L-lysine salt of the boronic acid. 
     48. A salt of any of paragraphs 1 to 28 which is a potassium salt. 
     49. A salt of any of paragraphs 1 to 28 which is a sodium salt. 
     50. A salt of any of paragraphs 1 to 28 which is a lithium salt. 
     51. A salt of any of paragraphs 1 to 28 which is an aminosugar salt. 
     52. A salt of paragraph 51 wherein the aminosugar is a ring-opened sugar. 
     53. A salt of paragraph 52 wherein the aminosugar is a glucamine. 
     54. A salt of paragraph 51 wherein the aminosugar is a cyclic aminosugar. 
     55. A salt of any of paragraphs 51 to 54 wherein the aminosugar is N-unsubstituted. 
     56. A salt of any of paragraphs 51 to 54 wherein the aminosugar is N-substituted by one or two substituents. 
     57. A salt of paragraph 56 wherein the or each substituent is a hydrocarbyl group. 
     58. A salt of paragraph 57 wherein the or each substituent Is selected from the group consisting of C 1 , C 2 , C 3 , C 4 , C 5 , C 6 , C 7  and C 8  alkyl groups 
     59. A salt of any of paragraphs 56 to 58 wherein there is a single N-substituent. 
     60. A salt of paragraph 51 wherein the glucamine is N-methyl-D-glucamine. 
     61. A salt of any of paragraphs 1 to 60 which comprises boronate ions derived from the peptide boronic acid and has a stoichiometry consistent with the boronate ions carrying a single negative charge. 
     62. A salt of any of paragraphs 1 to 60 wherein the salt consists essentially of acid salt in which one B—OH group of the boronate group, when trigonally represented, remains protonated. 
     63. A salt of any of paragraphs 1 to 62 wherein the salt comprises a boronate ion derived from the peptide boronic acid and a counter-ion and wherein the salt consists essentially of a salt having a single type of counter-ion. 
     64. A salt of any of paragraphs 1 to 28 which is a monolithium or monosodium salt. 
     65. A salt of paragraph 23 which is a monolithium or monosodium salt. 
     66. A solid phase monosodium or monolithium salt, containing no more than 1% water by weight, of Cbz-(R)-Phe-(S)-Pro-(R)-Mpg-B(OH) 2 . 
     66. A salt of paragraph 66 which contains no more than 0.5% water by weight. 
     68. A salt of paragraph 66 which contains no more than 0.1% water by weight. 
     69. A salt of any of paragraphs 66 to 68 which is the monosodium salt. 
     70. A salt of any of paragraphs 1 to 65 which is in the solid phase. 
     71. A salt of paragraph 70 which is substantially dry. 
     72. A product for use as a pharmaceutical, comprising a salt of any of paragraphs 1 to 71. 
     73. A pharmaceutical formulation in oral dosage form comprising a salt of any of paragraphs 1 to 71 and a pharmaceutically acceptable diluent, excipient or carrier. 
     74. A formulation of paragraph 73 which is a solid formulation. 
     75. A pharmaceutical formulation of paragraph 74 which is adapted to release the salt in the duodenum. 
     76. A pharmaceutical formulation of paragraph 75 which is enterically coated. 
     77. The use of a salt of any of paragraphs 1 to 71 for the manufacture of a medicament for treating thrombosis. 
     78. The use of paragraph 77 wherein the disease is an acute coronary syndrome. 
     79. The use of paragraph 77 wherein the disease is acute myocardial infarction. 
     80. The use of paragraph 77 wherein the disease is a venous thromboembolic event, selected from the group consisting of deep vein thrombosis and pulmonary embolism. 
     81. The use of a salt of any of paragraphs 1 to 71 for the manufacture of an oral medicament for preventing thrombosis in a haemodialysis circuit of a patient, for preventing a cardiovascular event in a patient with end stage renal disease, for preventing venous thromboembolic events in a patient receiving chemotherapy through an indwelling catheter, or for preventing thromboembolic events in a patient undergoing a lower limb arterial reconstructive procedure. 
     82. The use of a salt of any of paragraphs 1 to 71 for the manufacture of an oral medicament for treating by way of therapy or prophylaxis an arterial disease selected from acute coronary syndromes, cerebrovascular thrombosis, peripheral arterial occlusion and arterial thrombosis resulting from atrial fibrillation, valvular heart disease, arterio-venous shunts, indwelling catheters or coronary stents. 
     83. An oral pharmaceutical formulation comprising a combination of (i) a salt of any of paragraphs 1 to 71 and (ii) a further pharmaceutically active agent. 
     84. An oral pharmaceutical formulation comprising a combination of (i) a salt of any of paragraphs 1 to 71 and (ii) another cardiovascular treatment agent. 
     85. A formulation of paragraph 84 wherein the other cardiovascular treatment agent comprises a lipid-lowering drug, a fibrate, niacin, a statin, a CETP inhibitor, a bile acid sequestrant, an anti-oxidant, a IIb/IIIa antagonist, an aidosterone Inhibitor, an A2 antagonist, an A3 agonist, a betablocker, acetylsalicylic acid, a loop diuretic, an ace inhibitor, an antithrombotic agent with a different mechanism of action, an antiplatelet agent, a thromboxane receptor and/or synthetase inhibitor, a fibrinogen receptor antagonist, a prostacyclin mimetic, a phosphodiesterase inhibitor, an ADP-receptor (P 2 T) antagonist, a thrombolytic, a cardloprotectant or a COX-2 inhibitor. 
     86. A formulation of any of paragraphs 83 to 85 which is substantially dry. 
     87. The use of a salt of any of paragraphs 1 to 71 for the manufacture of a medicament for treating, for example preventing, a cardiovascular disorder in co-administration with another cardiovascular treatment agent. 
     88. A oral medicament comprising a salt of a boronic acid which is a selective thrombin inhibitor and has a neutral aminoboronic acid residue capable of binding to the thrombin Si subsite linked through a peptide linkage to a hydrophobic moiety capable of binding to the thrombin S2 and S3 subsites, the salt comprising a cation having a valency n and having an observed stoichiometry consistent with a notional stoichiometry (boronic acid:cation) of n:1. 
     89. A medicament of paragraph 88 wherein the boronic acid has a Ki for thrombin of about 100 nM or less, and optionally of about 20 nM or less. 
     90. A pharmaceutical formulation adapted for oral administration and comprising in the solid phase a compound which is a source of boronate species corresponding to the acid Cbz-(R)-Phe-(S)-Pro-(R)-Mpg-B(OH) 2  and a source of pharmaceutically acceptable cations other than choline and ammonium. 
     91. A method for forming a boronic acid of formula (I) or a product capable of being a source of such a boronic acid in vivo: 
     
       
         
         
             
             
         
       
     
     wherein 
     Y comprises a hydrophobic moiety which, together with the aminoboronic acid residue —NHCH(R 9 )—B(OH) 2 , has affinity for the substrate binding site of thrombin; and 
     R 9  is a straight chain alkyl group interrupted by one or more ether linkages and in which the total number of oxygen and carbon atoms is 3, 4, 5 or 6 or R 9  is —(CH 2 )m-W where m is from 2, 3, 4 or 5 and W is —OH or halogen (F, CI, Br or I), 
     the method comprising: 
     providing a diethylether solution of an ester of the acid; 
     dissolving diethanolamine in the solution; 
     allowing or causing a precipitate to form and recovering the precipitate, and 
     converting the precipitated material into an end product selected from the free organoboronic acid or a product capable when in vivo of being a source of such a boronic acid. 
     92. The method of paragraph 91, which further comprises formulating said end product into a pharmaceutical composition. 
     93. A method for forming a salt of any of paragraphs 1 to 71, comprising: combining an acetonitrile solution of the corresponding boronic add with a pharmaceutically acceptable base to form the salt. 
     94. A method of stabilising an organoboronic acid, comprising providing it in the form of a salt thereof. 
     95. A method of presenting an organoboronic add drug in stabilised form for pharmaceutical use, comprising providing the organoboronic acid drug in the form of a pharmaceutically acceptable base addition salt thereof in the solid phase.