Patent Publication Number: US-11020523-B2

Title: Canister stand

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This is a divisional of application Ser. No. 14/870,294, filed Sep. 30, 2015 and entitled SYSTEMS, APPARATUSES AND METHODS FOR TISSUE AND SUBSTANCE REMOVAL, DELIVERY AND/OR TRANSPLANTATION, which claims the benefit of U.S. provisional application No. 62/058,295, filed Oct. 1, 2014 and entitled SYSTEMS, APPARATUSES AND METHODS FOR TISSUE REMOVAL, DELIVERY AND/OR TRANSPLANTATION, each of which applications is hereby incorporated by reference herein in its entirety. 
    
    
     FIELD 
     Illustrative embodiments of the disclosure generally relate to systems, apparatuses and methods for removal, delivery and/or transplantation of body tissues and other substances in medical procedures. More particularly, illustrative embodiments of the disclosure relate to systems, apparatuses and methods for removal, delivery and/or transplantation of body tissues and other substances such as adipose tissue, preparation fluids, or filler materials for tissue rejuvenation, body shaping or other medical purposes. 
     BACKGROUND 
     The background description provided herein is solely for the purpose of generally presenting the context of the illustrative embodiments of the disclosure. Aspects of the background description are neither expressly nor impliedly admitted as prior art against the claimed subject matter. 
     Skin, adipose, musculoskeletal, and other body tissues may change in appearance and/or lose function with age and/or use due to injury, disease, wear, aging and/or other causes. One of the goals of plastic surgical procedures may include removal, delivery and/or transplantation of natural or man-made substances and body tissues in rejuvenation or shaping of the tissues to improve or restore the original appearance and/or function of the tissues. For example, conventional tissue shaping procedures may include liposuction, whereas tissue rejuvenation procedures may include the use of filler materials or adipose stem/progenitor cells to augment areas or promote angiogenesis and adipose/collagen tissue regeneration. However, such procedures may have less-than-optimum results and scarring. 
     Accordingly, systems, apparatuses and methods for removal, delivery and/or transplantation of substances or body tissues such as adipose tissue for tissue rejuvenation, body shaping or other medical purposes are needed. 
     SUMMARY 
     Illustrative embodiments of the disclosure are generally directed to a canister stand suitable for holding at least one dispensing canister in a system for removal, delivery and/or transplantation of body tissues and other substances in medical procedures. An illustrative embodiment of the canister stand may include a canister stand frame. A frame panel may be carried by the canister stand frame. At least one overhang may be formed by the canister stand frame. At least one canister opening may be provided in the frame panel at the at least one overhang. The at least one canister opening may be suitably sized and configured to accommodate the at least one dispensing canister. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       Illustrative embodiments of the disclosure will now be described, by way of example, with reference to the accompanying drawings, in which: 
         FIG. 1  is a diagram of an illustrative embodiment of the systems for tissue removal, delivery and/or transplantation; 
         FIG. 2  is an enlarged view of a distal end segment of a typical injection cannula; 
         FIG. 3  is a cross-sectional view, taken along section lines  3 - 3  in  FIG. 2 , of the typical injection cannula; 
         FIG. 4  is a side view of a typical dispensing canister according to some embodiments of the systems; 
         FIG. 5  is a side view of a typical canister stand according to some embodiments of the systems, with a dispensing canister and an accessory canister supported by the canister stand; 
         FIG. 6  is a top view of the typical canister stand with the dispensing canister and the accessory canister removed from the stand; 
         FIG. 7  is a side view of a typical pneumatic vibrational injection apparatus according to some embodiments of the systems; 
         FIG. 8  is a perspective view of a typical air-actuated transducer wheel of a pneumatic vibrational injection apparatus according to some embodiments of the systems; 
         FIG. 9  is an edge view of the typical transducer wheel; 
         FIG. 10  is a cross-sectional view illustrating an air inlet chamber, an air outlet chamber and a connecting chamber bypassing an injection chamber in the pneumatic vibrational injection apparatus according to some embodiments of the systems; 
         FIG. 10A  is a schematic block diagram of an illustrative embodiment of the systems for tissue removal, delivery and/or transplantation which utilizes a pneumatic vibrational injection apparatus; 
         FIG. 10B  is a side view of a typical hand-actuated pneumatic vibrational injection apparatus according to some embodiments of the systems; 
         FIG. 11  is a schematic diagram of an alternative illustrative embodiment of the systems for tissue removal, delivery and/or transplantation with a cannula instrument connected to a pump and a tissue-collecting container connected to the pump; 
         FIG. 12  is an enlarged view of a distal end segment of a typical aspiration cannula for the cannula instrument of  FIG. 11 ; 
         FIG. 13  is a perspective view of a typical handle for the cannula instrument of  FIG. 11 ; 
         FIG. 14  is a front view of the typical handle for the cannula instrument of  FIG. 11 ; and 
         FIG. 15  is a sectional perspective view of a typical handheld vibrational injection apparatus according to some embodiments of the systems. 
     
    
    
     DETAILED DESCRIPTION 
     The following detailed description is merely exemplary in nature and is not intended to limit the described embodiments or the application and uses of the described embodiments. As used herein, the word “exemplary” or “illustrative” means “serving as an example, instance, or illustration.” Any implementation described herein as “exemplary” or “illustrative” is not necessarily to be construed as preferred or advantageous over other implementations. All of the implementations described below are exemplary implementations provided to enable users skilled in the art to practice the disclosure and are not intended to limit the scope of the claims. Moreover, the illustrative embodiments described herein are not exhaustive and embodiments or implementations other than those which are described herein and which fall within the scope of the appended claims are possible. Furthermore, there is no intention to be bound by any expressed or implied theory presented in the preceding technical field, background, brief summary or the following detailed description. 
     Referring initially to  FIG. 1  of the drawings, an illustrative embodiment of the systems for tissue removal, delivery and/or transplantation, hereinafter system, is generally indicated by reference numeral  1 . The system  1  may include at least one dispensing canister  2 . A canister stand  24  may support the dispensing canister  2  for gravity-fed dispensing of material  42  such as tissue and/or fluid from the dispensing canister  2 . A pump  44  may be disposed in fluid communication with the dispensing canister  2  through sterile pump tubing  46 . A vibrational injection apparatus  50  may be disposed in fluid communication with the pump  44  through sterile apparatus tubing  48 . 
     In exemplary application of the system  1 , which will be hereinafter described, the dispensing canister  2  may contain a supply of material  42  which is to be injected into a recipient tissue underlying one or more anatomical sites on a patent (not illustrated) during a medical procedure. In some non-limiting applications, the material  42  may include a supply of crystalloid tumescent solution which is to be injected into the recipient tissue underlying one or more anatomical sites on the patient during a tumescent liposuction surgical procedure. In other non-limiting applications, the material  42  may include a supply of adipose tissue which is to be injected into the recipient tissue underlying one or more anatomical sites on the patient during an adipose tissue delivery or transplant surgical procedure. In still other non-limiting applications, the material  42  may include a soft-tissue filler such as hyaluronic acid and dispersion. In yet other non-limiting applications, the material  42  may include tissue regenerative cells such as adipose-derived stem cells (ASCs), pericytes and stem cells from bone marrow, for example and without limitation, in tissue transplantation procedures to regenerate damaged or deteriorated tissues in a patient. The pump  44  pumps the material  42  from the dispensing canister  2  to the vibrational injection apparatus  50 . A surgeon or other medical personnel (not illustrated) operates and manipulates the vibrational injection apparatus  50 , which vibrates as the material  42  is injected into the recipient tissue. The reciprocating or otherwise vibrating action of the vibrational injection apparatus  50  facilitates enhanced and uniform distribution of the material  42  throughout the recipient tissue, enabling the surgeon or medical personnel to achieve optimum results in the procedure. The vibrational injection apparatus  50  may include an injection cannula  60  having expanded tissue separation baskets  63 . The expanded tissue separation baskets  63  of the injection cannula  60 , in conjunction with the reduced areas  61  of the injection cannula  60  and the vibrating action of the vibrational injection apparatus  50 , facilitates recipient tissue expansion and preparation for deposition of the material  42 , further enhancing distribution and dispersion of the material  42  throughout the recipient tissues. 
     Referring next to  FIG. 4  of the drawings, the dispensing canister  2  of the system  1  may include a canister wall  3  which may be generally cylindrical in shape. The canister wall  3  may have a canister wall bottom edge  4  and a canister wall top edge  5 . A canister bottom panel  8  may be provided on the canister wall bottom edge  4  of the canister wall  3 . The canister bottom panel  8  may have a beveled, sloped or slanted orientation. In some embodiments, the slope of the canister bottom panel  8  may vary from between about 5 degrees to about 150 degrees relative to the canister wall  3 . 
     A dispensing spigot  10  may be disposed in fluid communication with the interior of the dispensing canister  2  at the lowest portion of the canister bottom panel  8  to facilitate connection of the pump tubing  46  ( FIG. 1 ) to the dispensing canister  2 . The dispensing spigot  10  may protrude downwardly from the canister bottom panel  8 . The dispensing spigot  10  may include multiple spigot protrusions  11  which grip and secure the pump tubing  46  to the dispensing spigot  10 . A removable canister lid  18  may be fitted on the canister wall top  5  of the canister wall  3 . In some embodiments, the dispensing canister  2  may have a volume of about 1-5 L. Graduated canister markings  14  may be provided on the canister wall  3  to indicate the volumes of the dispensing canister  2 . The dispensing canister  2  and its components may be fabricated of clear acrylic, plastic or ultra high molecular weight plastic, for example and without limitation, using conventional molding and/or other fabrication techniques known by those skilled in the art. The canister wall  3  may be transparent or clear to enable external visualization of the contents in the dispensing canister  2 . 
     In some embodiments, a canister handle  20  may protrude from the exterior surface of the canister wall  3  for carrying and handling purposes. The canister handle  20  may be detachably attached to the canister wall  3  through a clip-on, slide-on or other suitable handle attachment mechanism  21 , or may alternatively be fixed to the canister wall  3  according to the knowledge of those skilled in the art. 
     In some applications of the system  1 , the sloped canister bottom panel  8  of the dispensing canister  2  may facilitate separation of a lower layer of crystalloid and blood from overlying adipose tissue in the material  42  which is to be injected into the recipient tissue in the patient. The dispensing spigot  10  and the pump tubing  46 ,  48  may have a diameter or width which is sufficient to facilitate transfer of adipose tissue in the material  42  without blockage to the pump  44  and the vibrational injection apparatus  50 , respectively. In some non-limiting embodiments, the dispensing spigot  10  and the pump tubing  46 ,  48  may have a diameter of from about 3 mm to about 6 mm. 
     Referring next to  FIGS. 5 and 6  of the drawings, the canister stand  24  of the system  1  may include a canister stand frame  25 . In some embodiments, the canister stand frame  25  may be generally elongated and rectangular with a pair of side frame members  26  and a pair of end frame members  27  connecting the side frame members  26 . A frame panel  28  may be supported by and extend between the side frame members  26  and the end frame members  27 . As illustrated in  FIG. 6 , a first canister opening  30  and a second canister opening  32  may extend through the frame panel  28  in adjacent, spaced-apart relationship to each other. 
     A first set of parallel canister stand legs  34  may angle outwardly from the respective side frame members  26  beyond the end frame member  27  at a first end of the canister stand frame  25 . Angled foot portions  35  may terminate the respective canister stand legs  34  to support the canister stand legs  34  on a table or other flat support surface (not illustrated). A second set of parallel canister stand legs  34   a  may angle outwardly from the respective side frame members  26  beyond the end frame member  27  at a second end of the canister stand frame  25 . Angled foot portions  35  may terminate the respective canister stand legs  34   a  to support the canister stand legs  34   a  on the flat support surface (not illustrated). As illustrated in  FIG. 5 , each of the canister stand legs  34 ,  34   a  may extend through a leg opening (not illustrated) in a leg stabilizing bracket  38  which may extend outwardly from a corresponding side frame member  26  of the canister stand frame  25 . The various components of the canister stand  24  may be fabricated of stainless steel and/or other metal alloy or nonmetallic material which is suitable for the purpose of stabilizing the dispensing canister  2  as well as any accessory canisters  40  ( FIG. 5 ) during the medical procedure. 
     In some embodiments, at least one end of the canister stand frame  25  may protrude beyond at least the first set of canister stand legs  34  to form at least one overhang  29 . The first canister opening  32  may extend through the overhang  29  of the canister stand frame  25 . Throughout the medical procedure and during operation of the system  1 , the dispensing canister  2  may remain seated in the first canister opening  30  at the overhang  29 . The overhang  29  may facilitate gravity-fed flow of the material  42  directly from the dispensing container  2  through the dispensing spigot  10  into the pump tubing  46 . The overhang  29  may also facilitate placement of a biohazard waste receptacle (not illustrated) directly beneath the dispensing container  2  upon subsequent conclusion of the medical procedure. Accordingly, residual waste material  42  ( FIG. 1 ) which remains in the dispensing canister  2  can be selectively drained through the dispensing spigot  10  into the waste receptacle (not illustrated) and the residual waste material discarded. 
     The pump  44  ( FIG. 1 ) of the system  1  may be any type of pump which is suitable for the purpose of pumping materials and fluids in surgical procedures. Such pumps may include but are not limited to roller pumps or peristaltic pumps which are well known by those skilled in the art. In some applications, the operational rate of the pump  44  may be programmable and the pump  44  may have the capability to pump the material  42  from the dispensing canister  2  to the vibrational injection apparatus  50  at a rate of from between about 10 cc per minute and about 500 cc per minute. 
     The vibrational injection apparatus  50  of the system  1  may be any type of hand-held motorized device which receives the material  42  from the pump  44  through the apparatus tubing  48  and vibrates while delivering the material  42  through an injection cannula  60  into a recipient tissue which underlies one or more of the anatomical sites on the patient (not illustrated) in a tissue removal, injection, transplantation or other medical procedure. A non-limiting example of a motorized vibrational injection apparatus  50  which is suitable for the purpose is described in U.S. Patent Application Publication No. US2013/0310749, which is incorporated by reference herein in its entirety. Other non-limiting examples of vibrational injection apparatuses which are suitable for the purpose will be hereinafter described. Accordingly, the vibrational injection apparatus  50  may include an apparatus handle  51 . A vibration motor  52  may be provided in the apparatus handle  51 . A cannula connector  53  may terminate the apparatus handle  51 . The cannula connector  53  may facilitate coupling of the injection cannula  60  to the apparatus handle  51  such as through a luer lock connector or other suitable connection interface known by those skilled in the art. A power source connection  54  may extend from the apparatus handle  51  for connection to a suitable power source  58  such as through a power cord  59 . A switch (not illustrated) may be provided on the apparatus handle  51  or on the power cord  59  to facilitate selective manual operation of the vibration motor  52 . A tubing connector  55  may extend from the apparatus handle  51  for connection to the apparatus tubing  48  from the pump  44 . In some embodiments, the vibrational injection apparatus  50  may impart a reciprocating motion  56  ( FIG. 1 ) to the injection cannula  60 . The reciprocating motion  56  may have an actuated amplitude of from about 1 mm to about 5 mm, for example and without limitation. 
     The injection cannula  60  may include an elongated cannula shaft  61  which has a shaft lumen  62  ( FIG. 3 ) and terminates in a cannula tip  65 . In some embodiments, the injection cannula  60  may be an exploded basket cannula having at least one tissue separation basket  63  provided on the cannula shaft  61  proximal to the cannula tip  65 . In some embodiments, multiple tissue separation baskets  63  (such as 2-4 for example and without limitation) may be provided along the cannula shaft  61  in spaced-apart relationship to each other. Each tissue separation basket  63  may include multiple basket members  63   a  which may extend outwardly from the cannula shaft  61  in generally perpendicular relationship to each other. A cannula slot  63   b  ( FIGS. 2 and 3 ) may extend through the wall of the cannula shaft  61  between each basket member  63   a  and the shaft lumen  62 . The tissue separation baskets  63  may facilitate separation of and formation of multiple dispersion channels in the recipient tissue in the patient (not illustrated) for dispersion of the material  42  as the injection cannula  60  vibrates in the recipient tissue, as will be hereinafter further described. As illustrated in  FIG. 1 , in some embodiments, the injection cannula  60  incorporates a 5 to 20 degree angle placed in the long axis of the cannula  60 , enhancing the vibrating or reciprocating action by providing an element of short axis reciprocation in addition to the long axis reciprocation, effectively enhancing the vibrational action of the device  50 . In some embodiments, the injection cannula  60  may have a length of from about 20 cm to about 50 cm and the cannula shaft  61  may range in diameter from about 2 mm to about 5 mm. The injection cannula  60  may be fabricated of stainless steel, aluminum, ultra high molecular weight plastic and/or other disposable plastic or composite material. In some embodiments, the injection cannula  60  may have a length of about 2 cm to 20 cm and the canula shaft  61  may range in diameter from about 32 gauge to about 12 gauge (needle gauge). 
     In exemplary application, the system  1  facilitates injection of a material  42  such as a supply of crystalloid tumescent solution which is to be percutaneously or subcutaneously injected into a recipient tissue which underlies one or more anatomical sites on a patient during or preparatory to a tumescent liposuction surgical procedure or a supply of adipose tissue which is to be injected into the recipient tissue during an adipose tissue delivery or transplant surgical procedure. Accordingly, the material  42  may be placed in the dispensing container  2 . The canister stand  24  may be placed on a table or other suitable support (not illustrated). The dispensing container  2  may be seated in the first canister opening  30  ( FIG. 6 ) in the overhang  29  of the canister stand  24 . 
     The pump tubing  46  may be connected, to the dispensing spigot  10  on the dispensing canister  2  and to the pump  44 . The apparatus tubing  48  may be connected to the pump  44  and the tubing connector  55  on the vibrational injection apparatus  50 . The vibrational injection apparatus  50  may be connected to the power source  58  through the power source connection  54 . 
     An incision (not illustrated) is made at the anatomical site on the patient which overlies the recipient tissue into which the material  42  is to be injected. A surgeon (not illustrated) grips the apparatus handle  51  and inserts the injection cannula  60  into the incision. The vibration motor  52  may be operated to vibrate the injection cannula  60  typically according to the actuated amplitude of the reciprocating motion  56 . As the pump  44  is operated to pump the material  42  from the dispensing canister  2  through the injection cannula  60  into the recipient tissue in the patient, the vibrating tissue separation baskets  63  on the injection cannula  60  may facilitate mechanical separation of connective tissue and create expanded dispersion channels in the recipient tissue which enhance diffusion of the material  42  throughout the tissue. The vibrating action of the injection cannula  60  may also facilitate egress of the material  42  from the injection cannula  60  into the recipient tissue. This effect may expedite removal of adipose tissue in all types of liposuction procedures including but not limited to percutaneous liposuction and liposuction directly into subcutaneous adipose tissue during liposuction and transplantation procedures. 
     In some applications, the system  1  may be implemented in tissue transplantation procedures to regenerate damaged or deteriorated tissues in a patient. Thus, the material  42  may include tissue regenerative cells such as adipose-derived stem cells (ASCs), pericytes and stem cells from bone marrow, for example and without limitation, which may initially be removed from donor tissue underlying one or more anatomical sites on the patient using conventional liposuction instruments and techniques. The material  42  which was removed from the donor tissue may subsequently be placed into the dispensing canister  2  and injected into the recipient tissue underlying one or more other anatomical sites on the patient using the vibrational injection apparatus  50  to infiltrate the recipient tissue or tissues and mediate release of soluble factors that stimulate adipogenesis and angiogenesis for rejuvenation treatment in the recipient tissue. The vibrating action of the injection cannula  60  may increase the quantity or proportion of the tissue regenerative cells which are subject to injury before and as they enter the recipient tissue. The injured tissue regenerative cells may release soluble factors which recruit and activate other tissue regenerative cells and bone-marrow derived stem cells in the recipient tissue. The released factors may promote angiogenesis and adipogenesis, filling and restoring the original appearance of the anatomical site which overlies the recipient tissue. This procedure may be carried out as described in co-pending U.S. application Ser. No. 14/173,021, filed Feb. 5, 2014 and entitled TISSUE REJUVENATION METHODS AND TISSUE TRANSFER DEVICES SUITABLE FOR IMPLEMENTATION THEREOF, which application is incorporated by reference herein in its entirety. 
     Upon conclusion of the injection procedure, the vibration motor  52  may be turned off and the injection cannula  60  removed from the recipient tissue in the patient. Any residual material  42  which remains in the dispensing canister  2  may be drained through the dispensing spigot  10  into a biohazard waste receptacle (not illustrated) which is placed directly beneath the dispensing canister  2  after detachment of the pump tubing  46 . Alternatively, the residual material  42  may be decanted from the dispensing canister  2  by gripping the canister handle  20  and pouring the residual material  42  into the waste receptacle. 
     Referring next to  FIGS. 7-10A  of the drawings, an alternative illustrative embodiment of the systems  101  ( FIG. 10A ) which utilizes a pneumatic vibrational injection apparatus  150  is illustrated. The pneumatic vibrational injection apparatus  150  may include an apparatus housing  170 . As illustrated in  FIG. 7 , the apparatus housing  170  may include an air inlet chamber  171 , an air outlet chamber  172  and a connecting chamber  173  which connects the air outlet chamber  172  to the air inlet chamber  171 . As further illustrated in  FIG. 7 , in operation of the apparatus  150 , which will be hereinafter described, air  168  flows through the air inlet chamber  171 , the connecting chamber  173  and the air outlet chamber  172 , respectively, of the apparatus housing  170  for purposes which will be hereinafter described. 
     An injection chamber  176  may be disposed between the air inlet, chamber  171  and the air outlet chamber  172 . As illustrated in  FIG. 10 , the injection chamber  176  may bypass the connecting chamber  173  at a forward end of the apparatus housing  170 . An injection nozzle  177  may be disposed in fluid communication with the injection chamber  176 . The injection nozzle  177  may extend from the forward end of the apparatus housing  170 . An injection cannula  179  may be coupled to the injection nozzle  177  through a suitable needle connector  178  such as a luer-lock connector, for example and without limitation. 
     An inlet transducer wheel  180  and an outlet transducer wheel  180   a  may be mounted for rotation in the air inlet chamber  171  and the air outlet chamber  172 , respectively, of the apparatus housing  170  such as via respective wheel axles  183 . As illustrated in  FIG. 9 , each of the inlet transducer wheel  180  and the outlet transducer wheel  180   a  may include a pair of spaced-apart transducer wheel disks  181 . Multiple transducer wheel blades  182  may extend in radial orientation from the wheel axle  183  outwardly between the transducer wheel disks  181  in spaced-apart relationship to each other and around the circumference of the corresponding inlet transducer wheel  180  and outlet transducer wheel  180   a.    
     As illustrated in  FIG. 7 , an inlet air flow director  186  may be provided in the air inlet chamber  171  of the apparatus housing  170 , upstream of the inlet transducer wheel  180  relative to the direction of the flow of air  168  through the apparatus housing  170 . The inlet air flow director  186  may direct the air  168  which flows through the air inlet chamber  171  against the transducer wheel blades  182  of the inlet transducer wheel  180  such that the inlet transducer wheel  180  rotates in a first direction, such as the clockwise direction in the non-limiting example illustrated in  FIG. 7 . An outlet air flow director  186   a  may likewise be provided in the air outlet chamber  172  upstream of the outlet transducer wheel  180   a . The outlet air flow director  186   a  may direct the air  168  which flows through the air outlet chamber  172  against the transducer wheel blades  182  of the outlet transducer wheel  180   a  such that the outlet transducer wheel  180   a  rotates in a second direction, such as the counterclockwise direction in the non-limiting example illustrated in  FIG. 7 . 
     A vibration transducer  190  may be disposed in physical contact with the inlet transducer wheel  180  and the outlet transducer wheel  180   a . The injection nozzle  177  may be disposed in physical contact with the vibration transducer  190 . In some embodiments, the vibration transducer  190  may include an inlet transducer arm  191  which extends from the inlet transducer wheel  180  and an outlet transducer arm  191   a  which extends from the outlet transducer wheel  180   a . A transducer bridge  192  may extend between the inlet transducer arm  191  and the outlet transducer arm  191   a . The injection nozzle  177  may extend through a nozzle opening (not illustrated) in the transducer bridge  192 . Accordingly, responsive to flow of air  168  through the air inlet chamber  171 , the connecting chamber  173  and the air outlet chamber  172 , respectively, the inlet transducer wheel  180  may rotate in the first direction to apply a pushing motion to the inlet transducer arm  191 . Conversely, the outlet transducer wheel  180   a  may rotate in the second direction to simultaneously apply a pulling, motion to the outlet transducer arm  191   a . This simultaneous antagonistic pushing and pulling action on the inlet transducer arm  191  and the outlet transducer arm  191   a , respectively, imparts vibration to the vibration transducer  190 , which in turn imparts vibration to the injection nozzle  177  and the injection cannula  179 . 
     As illustrated in  FIG. 10A , a compressed air source  166  may be disposed in pneumatic communication with the air inlet chamber  171  and the air outlet chamber  172 , respectively, of the apparatus housing  170  through a pair of compressed air lines  167 . The compressed air lines  167  may be detachably coupled to the respective air inlet chamber  171  and the air outlet chamber  172  through any type of airtight hose coupling which is known by those skilled in the art and suitable for the purpose. A pump  144 , such as the peristaltic pump  44  which was heretofore described with respect to  FIG. 1 , for example and without limitation, may be disposed in fluid communication with the injection chamber  176  of the apparatus housing  170  through apparatus tubing  148 . A dispensing canister  102 , such as the dispensing canister  2  which was heretofore described with respect to  FIG. 4 , may be disposed in fluid communication with the pump  144  through pump tubing  146 . 
     Operation of the pneumatic vibrational injection apparatus  150  of the system  101  may be as was heretofore described with respect to operation of the motorized vibrational apparatus  50  of the system  1  in  FIG. 1 . As illustrated in  FIG. 7 , the flow of air  168  through the air inlet chamber  171 , the connecting chamber  173  and the air outlet chamber  172 , respectively, imparts vibration to the injection cannula  179  through the vibration transducer  170  as was heretofore described. 
     Referring next to  FIG. 10B  of the drawings, a typical alternative, hand-actuated pneumatic vibrational injection apparatus  150   a  which may be used in implementation of system  101  in  FIG. 10A  is illustrated. In the hand-operated pneumatic vibrational injection apparatus  150   a , a syringe plunger  164  may slidably engage the injection chamber  176  in the apparatus housing  170  instead of the pump  144  being disposed in fluid communication with the injection chamber  176  as was heretofore described with respect to the system  101  illustrated in  FIG. 10A . Accordingly, the material  42  ( FIG. 1 ) which is injected into the recipient tissue in the patient may first be drawn into the injection chamber  176  through the injection cannula  179  by drawing a vacuum on the injection chamber  176  through rearward displacement of the syringe plunger  164 . The material  42  may be expelled from the injection chamber  176  through the injection cannula  179  into the recipient tissue by subsequently generating positive pressure in the injection chamber  176  through forward displacement of the syringe plunger  164 . Simultaneously, air  168  may be distributed through the air inlet chamber  171 , the connecting chamber  173  and the air outlet chamber  172 , respectively, of the apparatus housing  170 , typically through the compressed, air lines  167  coupled to the compressed air source  166  ( FIG. 10A ), to impart vibration to the injection cannula  179  as was heretofore described with respect to operation of the vibrational injection apparatus  150  in  FIG. 7 . 
     Referring next to  FIGS. 11-14  of the drawings, an alternative illustrative embodiment of a system  201  with a cannula instrument  294  connected to a pump  244  and a tissue-collecting container  202  is illustrated. The cannula instrument  294  may include a lightweight cannula handle  295 . An aspiration cannula  400  may extend from the cannula handle  295 . In some embodiments, the aspiration cannula  400  may have a length of from about 30 cm to about 50 cm and a diameter of from about 3 mm to about 5 mm. As illustrated in  FIG. 12 , the aspiration cannula  400  may include a series of staggered cannula openings  401  proximal to the cannula tip  402 . Each of the cannula openings  401  may have a circular, elliptical or other shape. In some embodiments, the aspiration cannula  400  may include about 10-14 cannula openings  401 . The aspiration cannula  400  may be stainless steel, aluminum, clear plastic such as ultrahigh molecular weight plastic and/or other suitable material. 
     As illustrated in  FIGS. 13 and 14 , the cannula handle  295  of the cannula instrument  294  may have a fenestrated or caged design with a pair of spaced-apart handle end frame members  296 . Multiple elongated handle side frame members  297  may extend between the handle end frame members  296  in parallel, spaced-apart relationship to each other along the length of the cannula handle  295 . Multiple handle middle frame members  298  may extend between the handle side frame members  297  in spaced-apart relationship to each other between the handle end frame members  296 . An elongated tubing receptacle  299  may extend through and in generally concentric relationship to the handle end frame members  296  and the handle middle frame members  298 . Connecting frame members  300  may extend between the tubing receptacle  299  and the handle end frame members  296  and between the tubing receptacle  299  and one or more of the handle middle frame members  298  to minimize structure and material which connects the pump tubing  246  to the cannula handle  295 . The cannula handle  295  may include lightweight aluminum, steel and/or other metal alloy and/or plastic or other suitable lightweight rigid material. 
     As illustrated in  FIG. 11 , in exemplary application, the aspiration cannula  400  may be connected to the cannula handle  295  via a luer lock or other suitable cannula connector  404 . Apparatus tubing  248  may connect a pump  244  to the aspiration cannula  400  through the cannula connector  404 . Pump tubing  246  may connect the pump  244  to a container  202 . The apparatus tubing  248  may extend through the tubing receptacle  299  centralized along the length of the cannula handle  295 . Accordingly, during a liposuction or other medical procedure, a surgeon (not illustrated) grips the cannula handle  295  and inserts the aspiration cannula  400  through an incision (not illustrated) into recipient tissue which underlies at one or more anatomical sites on a patient. The pump  244  may be operated to suction adipose tissue from the patient through the aspiration cannula  400 , the apparatus tubing  248  and the pump tubing  246 , respectively, into the container  202 . The surgeon may repeatedly move the aspiration cannula  400  back and forth in multiple strokes to ensure that adipose tissue is adequately and uniformly removed from the desired anatomical area of the patient. It will be appreciated by those skilled in the art that the lightweight construction of the cannula handle  295  reduces the work which the surgeon is required to exert with each stroke of the aspiration cannula  400  within the anatomical area of the patient which is being shaped. Accordingly, the lightweight cannula handle  295  prevents the surgeon from becoming fatigued, particularly in liposuction procedures in which large quantities of adipose tissue are removed from the patient. 
     Referring next to  FIG. 15  of the drawings, another typical handheld vibrational injection apparatus  550  according to some embodiments of the systems is illustrated. The vibrational injection apparatus  550  may include an apparatus housing  570  which may be generally elongated. The apparatus housing  570  may include a vibration mechanism compartment  571  and an adjacent injector compartment  572 . A vibration mechanism  576  may be provided in the vibration mechanism compartment  571  and a material injector  584  may be provided in the injector compartment  572  of the apparatus housing  570 . 
     The vibration mechanism  576  may include any type of mechanical, pneumatic or other device which is capable of imparting vibration to the material injector  584  typically in a manner which will be hereinafter described. In some embodiments, the vibration mechanism  576  may include the pneumatic vibrating mechanism which was heretofore described with respect to the vibrational injection apparatuses  150 ,  150   a  in  FIGS. 7-10B . In other embodiments, the vibration mechanism  576  may include a vibration mechanism motor  577 . A battery compartment  580  may be provided in the vibration mechanism compartment  571  adjacent to the vibration mechanism motor  577 . The battery compartment  580  may be electrically connected to the vibration mechanism motor  577  and may contain at least one battery (not illustrated) which supplies electrical current for operation of the vibration mechanism motor  577 . The vibration mechanism motor  577  may drivingly engage a vibration shaft  578  for reciprocation of the vibration shaft  578  according to the knowledge of those skilled in the art. An injector vibrating shaft  560  may be engaged for reciprocation by the vibration shaft  578 . A shaft connector  566  may couple the injector vibrating shaft  560  to the vibration shaft  578 . 
     The material injector  584  may include any type of mechanism, structure or device which is suitable for the purpose of receiving or containing a material  42  ( FIG. 1 ) and facilitating injection of the material into the patient or removing the material  42  from the patient. The material injector  584  may include at least one injection chamber  585 . In some embodiments, a hand-operated or automated plunger  586  may terminate in a plunger head  587  which slides within the injection chamber  585  to facilitate selective drawing of the material  42  into or expulsion of the material  42  from the injection chamber  585 . In other embodiments, a pump  44  ( FIG. 1 ) may be connected in fluid communication with the injection chamber  585  through apparatus tubing  48 . A dispensing canister  2  may be connected in fluid communication with the pump  44  and may contain the material  42  which is to be injected into the patient. 
     A telescoping injector tube  588  in the injector compartment  572  of the apparatus housing  570  may be disposed in fluid communication with the injection chamber  585 . A cannula connector  590  may be disposed in fluid communication with the injector tube  588  on the exterior of the apparatus housing  570 . The cannula connector  590  may facilitate coupling of an injection cannula  592  to the material injector  584  such as through a luer lock connector or other suitable connection interface known by those skilled in the art. In some embodiments, the injection cannula  592  may have a length of from about 2 cm to about 20 cm and the cannula shaft of the injection cannula  592  may range in diameter from about 32 gauge to about 12 gauge (needle gauge). 
     A driver  554  may couple the injector vibrating shaft  560  of the vibration mechanism  576  to the injector tube  588  of the material injector  584 . The driver  554  may include a generally elongated driver bridge  555  and, a driver head  556  which terminates the driver bridge  555 . The reciprocating vibration shaft  560  may extend through a shaft opening (not illustrated) in the driver bridge  555  and may engage the driver bridge  555  for reciprocation of the driver  554  with the reciprocating vibration shaft  560 . The injector tube  588  may communicate with a tube opening (not illustrated) in the driver head  556  and may extend and retract in a telescoping motion with the reciprocating driver  554 . A spring stop  561  may terminate the vibration shaft  560 . A driver return spring  562  may be interposed between the driver bridge  555  and the spring stop  561 . Accordingly, in operation of the vibrational injection apparatus  550 , the vibration motor  577  repeatedly reciprocates the reciprocating vibration shaft  578  and the injector vibrating shaft  560  between retracted and extended positions. The driver bridge  555  and the driver head  556  of the driver  554  reciprocate with the injector vibrating shaft  560 . Thus, the driver head  556  of the driver  554  reciprocates the telescoping injector tube  588 , which telescopes and vibrates the material  42  as it flows from the injector chamber  585  through the injector tube  588  and the injection cannula  592  coupled to the cannula connector  590  and is discharged from the injection cannula  592  into the patient (not illustrated). 
     Application of the vibrational injection apparatus  550  may be as was heretofore described with respect to the vibrational injection apparatus  50  in the system  1  illustrated in  FIG. 1 . Accordingly, material  42  may be drawn into the injection chamber  585  of the material injector  584  through the injection cannula  592  by actuation of the plunger  586 . The material  42  may include a supply of crystalloid tumescent solution which is to be percutaneously or subcutaneously injected into a recipient tissue which underlies one or more anatomical sites on a patient during or preparatory to a tumescent liposuction surgical procedure or a supply of adipose tissue which is to be injected into the recipient tissue during an adipose tissue delivery or transplant surgical procedure. Alternatively, the material  42  may include a soft-tissue filler such as hyaluronic acid and dispersion which is to be injected in a patient in body shaping procedures. Still further in the alternative, the material  42  may include tissue regenerative cells such as adipose-derived stem cells (ASCs), pericytes and stem cells from bone marrow, for example and without limitation, in tissue transplantation procedures to regenerate damaged or deteriorated tissues in a patient. 
     The vibration mechanism motor  577  may be operated to vibrate the telescoping injector tube  588  of the material injector  584  through the reciprocating the vibration shaft  578 , the injector vibrating shaft  560  and the driver  554  as the injector  584  is operated to expel the material  42  from the injection chamber  585  through the injector tube  588 , the driver head  556  and the cannula connector  590  and into the injection cannula  592 , respectively. As the material  42  is discharged from the injection cannula  592  into the patient, the vibrating motion of the injector tube  588  is transmitted to the injector cannula  592  through the driver head  556  and the cannula connector  590 . The vibrating injection cannula  592  may facilitate mechanical separation of connective tissue and create expanded dispersion channels in the recipient tissue which enhance diffusion of the material  42  throughout the tissue, facilitate egress of the material  42  from the injection cannula  592  into the recipient tissue and/or increase the quantity or proportion of tissue regenerative cells which are in the material  42  and subject to injury before and as they enter the recipient tissue. Thus, the injured tissue regenerative cells may release soluble factors which recruit and activate other tissue regenerative cells and bone-marrow derived stem cells in the recipient tissue. The released factors may promote angiogenesis and adipogenesis, filling and restoring the original appearance of the anatomical site which overlies the recipient tissue. 
     While illustrative embodiments of the disclosure have been described above, it will be recognized and understood that various modifications can be made in the disclosure and the appended claims are intended to cover all such modifications which may fall within the spirit and scope of the disclosure.