Patent Publication Number: US-2021180057-A1

Title: MODULATORS OF ENaC EXPRESSION

Description:
SEQUENCE LISTING 
     The present application is being filed along with a Sequence Listing in electronic format. The Sequence Listing is provided as a file entitled BIOL0315WOSEQ.txt created Oct. 10, 2018 which is 484 kb in size. The information in the electronic format of the sequence listing is incorporated herein by reference in its entirety. 
     FIELD 
     The present embodiments provide methods, compounds, and compositions useful for inhibiting ENaC expression, which can be useful for treating, preventing, or ameliorating a disease associated with ENaC. 
     BACKGROUND 
     The epithelial sodium channel (ENaC) is a channel made up of three subunits (typically α-ENaC, β-ENaC, and γ-ENaC; or SCNN1A, SCNN1B, and SCNN1G, respectively) that is expressed in several tissues, including the lungs. It allows passage of sodium ions across the epithelial cell membrane and is negatively regulated by chloride ions. In cystic fibrosis patients, the inhibition of ENaC is reduced due to decreased function of the chloride transporter, CFTR. 
     SUMMARY 
     Certain embodiments provided herein are directed to potent and tolerable compounds and compositions useful for inhibiting ENaC expression, which can be useful for treating, preventing, ameliorating, or slowing progression of lung disorders, e.g., cystic fibrosis, chronic obstructive pulmonary disease (COPD), chronic bronchitis, and asthma. Certain embodiments provided herein comprise modified oligonucleotides complementary to an α-ENaC nucleic acid that potently reduce α-ENaC expression in animals. 
    
    
     DETAILED DESCRIPTION 
     It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the embodiments, as claimed. Herein, the use of the singular includes the plural unless specifically stated otherwise. As used herein, the use of “or” means “and/or” unless stated otherwise. Furthermore, the use of the term “including” as well as other forms, such as “includes” and “included”, is not limiting. 
     The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described. All documents, or portions of documents, cited in this application, including, but not limited to, patents, patent applications, articles, books, treatises, and GenBank and NCBI reference sequence records are hereby expressly incorporated by reference for the portions of the document discussed herein, as well as in their entirety. 
     It is understood that the sequence set forth in each SEQ ID NO contained herein is independent of any modification to a sugar moiety, an internucleoside linkage, or a nucleobase. As such, compounds defined by a SEQ ID NO may comprise, independently, one or more modifications to a sugar moiety, an internucleoside linkage, or a nucleobase. 
     As used herein, “2′-deoxynucleoside” means a nucleoside comprising 2′-H(H) ribosyl sugar moiety, as found in naturally occurring deoxyribonucleic acids (DNA). In certain embodiments, a 2′-deoxynucleoside may comprise a modified nucleobase or may comprise an RNA nucleobase (uracil). 
     As used herein, “2′-substituted nucleoside” or “2-modified nucleoside” means a nucleoside comprising a 2′-substituted or 2′-modified sugar moiety. As used herein, “2′-substituted” or “2-modified” in reference to a furanosyl sugar moiety means a sugar moiety comprising at least one 2′-substituent group other than H or OH. 
     As used herein, “administration” or “administering” refers to routes of introducing a compound or composition provided herein to an individual to perform its intended function. An example of a route of administration that can be used includes, but is not limited to, administration by inhalation. 
     As used herein, “administered concomitantly” or “co-administration” means administration of two or more compounds in any manner in which the pharmacological effects of both are manifest in the patient. Concomitant administration does not require that both compounds be administered in a single pharmaceutical composition, in the same dosage form, by the same route of administration, or at the same time. The effects of both compounds need not manifest themselves at the same time. The effects need only be overlapping for a period of time and need not be coextensive. Concomitant administration or co-administration encompasses administration in parallel or sequentially. 
     As used herein, “animal” refers to a human or non-human animal, including, but not limited to, mice, rats, rabbits, dogs, cats, pigs, and non-human primates, including, but not limited to, monkeys and chimpanzees. 
     As used herein, “antisense activity” means any detectable and/or measurable change attributable to the hybridization of an antisense compound to its target nucleic acid. In certain embodiments, antisense activity is a decrease in the amount or expression of a target nucleic acid or protein encoded by such target nucleic acid compared to target nucleic acid levels or target protein levels in the absence of the antisense compound. 
     As used herein, “antisense compound” means a compound comprising an antisense oligonucleotide and optionally one or more additional features, such as a conjugate group or terminal group. 
     As used herein, “antisense oligonucleotide” means an oligonucleotide having a nucleobase sequence that is at least partially complementary to a target nucleic acid. 
     As used herein, “ameliorate” in reference to a treatment means improvement in at least one symptom relative to the same symptom in the absence of the treatment. In certain embodiments, amelioration is the reduction in the severity or frequency of a symptom or the delayed onset or slowing of progression in the severity or frequency of a symptom. 
     As used herein, “bicyclic nucleoside” or “BNA” means a nucleoside comprising a bicyclic sugar moiety. As used herein, “bicyclic sugar” or “bicyclic sugar moiety” means a modified sugar moiety comprising two rings, wherein the second ring is formed via a bridge connecting two of the atoms in the first ring thereby forming a bicyclic structure. In certain embodiments, the first ring of the bicyclic sugar moiety is a furanosyl moiety. In certain embodiments, the bicyclic sugar moiety does not comprise a furanosyl moiety. 
     As used herein, “cEt” or “constrained ethyl” means a bicyclic sugar moiety, wherein the first ring of the bicyclic sugar moiety is a ribosyl sugar moiety, the second ring of the bicyclic sugar is formed via a bridge connecting the 4′-carbon and the 2′-carbon, the bridge has the formula 4′-CH(CH 3 )—O-2′, and the methyl group of the bridge is in the S configuration. A cEt bicyclic sugar moiety is in the β-D configuration. 
     As used herein, “chirally enriched population” means a plurality of molecules of identical molecular formula, wherein the number or percentage of molecules within the population that contain a particular stereochemical configuration at a particular chiral center is greater than the number or percentage of molecules expected to contain the same particular stereochemical configuration at the same particular chiral center within the population if the particular chiral center were stereorandom. Chirally enriched populations of molecules having multiple chiral centers within each molecule may contain one or more sterorandom chiral centers. In certain embodiments, the molecules are modified oligonucleotides. In certain embodiments, the molecules are compounds comprising modified oligonucleotides. 
     As used herein, “complementary” in reference to an oligonucleotide means that at least 70% of the nucleobases of such oligonucleotide or one or more regions thereof and the nucleobases of another nucleic acid or one or more regions thereof are capable of hydrogen bonding with one another when the nucleobase sequence of the oligonucleotide and the other nucleic acid are aligned in opposing directions. Complementary nucleobases are nucleobase pairs that are capable of forming hydrogen bonds with one another. Complementary nucleobase pairs include adenine (A) and thymine (T), adenine (A) and uracil (U), cytosine (C) and guanine (G), 5-methyl cytosine ( m C) and guanine (G). Complementary oligonucleotides and/or nucleic acids need not have nucleobase complementarity at each nucleoside. Rather, some mismatches are tolerated. As used herein, “fully complementary” or “100% complementary” in reference to oligonucleotides means that such oligonucleotides are complementary to another oligonucleotide or nucleic acid at each nucleoside of the oligonucleotide. 
     As used herein, “conjugate group” means a group of atoms that is directly or indirectly attached to an oligonucleotide. Conjugate groups include a conjugate moiety and a conjugate linker that attaches the conjugate moiety to the oligonucleotide. 
     As used herein, “conjugate linker” means a group of atoms comprising at least one bond that connects a conjugate moiety to an oligonucleotide. 
     As used herein, “conjugate moiety” means a group of atoms that is attached to an oligonucleotide via a conjugate linker. 
     As used herein, “contiguous” in the context of an oligonucleotide refers to nucleosides, nucleobases, sugar moieties, or internucleoside linkages that are immediately adjacent to each other. For example, “contiguous nucleobases” means nucleobases that are immediately adjacent to each other in a sequence. 
     As used herein, “double-stranded antisense compound” means an antisense compound comprising two oligomeric compounds that are complementary to each other and form a duplex, and wherein one of the two said oligomeric compounds comprises an antisense oligonucleotide. 
     As used herein, “effective amount” means the amount of compound sufficient to effectuate a desired physiological outcome in an individual in need of the compound. The effective amount may vary among individuals depending on the health and physical condition of the individual to be treated, the taxonomic group of the individuals to be treated, the formulation of the composition, assessment of the individual&#39;s medical condition, and other relevant factors. 
     As used herein, “efficacy” means the ability to produce a desired effect. 
     As used herein “ENaC” means any ENaC (epithelial sodium channel) nucleic acid or protein. “ENaC nucleic acid” means any nucleic acid encoding an ENaC subunit. For example, in certain embodiments, an ENaC nucleic acid includes a DNA chromosomal region encoding ENaC, an RNA transcribed from DNA encoding ENaC (e.g., a pre-mRNA transcript), and an mRNA transcript encoding ENaC. In certain embodiments, an ENaC nucleic acid or protein is an α-ENaC or SCNN1A (sodium channel epithelial 1 alpha subunit) nucleic acid or protein. Herein, α-ENaC and SCNN1A are used interchangeably and have the same meaning. 
     As used herein, “expression” includes all the functions by which a gene&#39;s coded information is converted into structures present and operating in a cell. Such structures include, but are not limited to, the products of transcription and translation. 
     As used herein, “gapmer” means an oligonucleotide, such as an antisense oligonucleotide, comprising an internal segment having a plurality of nucleosides that support RNase H cleavage positioned between external segments, each having one or more nucleosides, wherein the nucleosides comprising the internal segment are chemically distinct from the immediately adjacent nucleoside or nucleosides comprising the external segments. The internal segment may be referred to as the “gap” or “gap segment” and the external segments may be referred to as the “wings” or “wing segments”. 
     As used herein, “hybridization” means the pairing or annealing of complementary oligonucleotides and/or nucleic acids. While not limited to a particular mechanism, the most common mechanism of hybridization involves hydrogen bonding, which may be Watson-Crick, Hoogsteen or reversed Hoogsteen hydrogen bonding, between complementary nucleobases. 
     As used herein, “individual” means a human or non-human animal selected for treatment or therapy. 
     As used herein, “inhibiting the expression or activity” refers to a reduction or blockade of the expression or activity relative to the expression or activity in an untreated or control sample and does not necessarily indicate a total elimination of expression or activity. 
     As used herein, the terms “internucleoside linkage” means a group or bond that forms a covalent linkage between adjacent nucleosides in an oligonucleotide. As used herein “modified internucleoside linkage” means any internucleoside linkage other than a naturally occurring, phosphate internucleoside linkage. Non-phosphate linkages are referred to herein as modified internucleoside linkages. “Phosphorothioate linkage” means a modified phosphate linkage in which one of the non-bridging oxygen atoms is replaced with a sulfur atom. A phosphorothioate internucleoside linkage is a modified internucleoside linkage. Modified internucleoside linkages include linkages that comprise abasic nucleosides. As used herein, “abasic nucleoside” means a sugar moiety in an oligonucleotide or oligomeric compound that is not directly connected to a nucleobase. In certain embodiments, an abasic nucleoside is adjacent to one or two nucleosides in an oligonucleotide. 
     As used herein, “linker-nucleoside” means a nucleoside that links, either directly or indirectly, an oligonucleotide to a conjugate moiety. Linker-nucleosides are located within the conjugate linker of an oligomeric compound. Linker-nucleosides are not considered part of the oligonucleotide portion of an oligomeric compound even if they are contiguous with the oligonucleotide. 
     As used herein, “non-bicyclic modified sugar” or “non-bicyclic modified sugar moiety” means a modified sugar moiety that comprises a modification, such as a substitutent, that does not form a bridge between two atoms of the sugar to form a second ring. 
     As used herein, “linked nucleosides” are nucleosides that are connected in a continuous sequence (i.e. no additional nucleosides are present between those that are linked). 
     As used herein, “mismatch” or “non-complementary” means a nucleobase of a first oligonucleotide that is not complementary with the corresponding nucleobase of a second oligonucleotide or target nucleic acid when the first and second oligomeric compound are aligned. 
     As used herein, “modulating” refers to changing or adjusting a feature in a cell, tissue, organ or organism. For example, modulating ENaC expression can mean to increase or decrease the level of an ENaC RNA and/or an ENaC protein in a cell, tissue, organ or organism. A “modulator” effects the change in the cell, tissue, organ or organism. For example, a compound that modulates ENaC expression can be a modulator that decreases the amount of an ENaC RNA and/or an ENaC protein in a cell, tissue, organ or organism. 
     As used herein, “MOE” means methoxyethyl. “2′-MOE” or “2′-O-methoxyethyl” means a 2′-OCH 2 CH 2 OCH 3  group in place of the 2′-OH group of a ribosyl ring. 
     As used herein, “motif” means the pattern of unmodified and/or modified sugar moieties, nucleobases, and/or internucleoside linkages, in an oligonucleotide. 
     As used herein, “naturally occurring” means found in nature. 
     As used herein, “nucleobase” means an unmodified nucleobase or a modified nucleobase. As used herein an “unmodified nucleobase” is adenine (A), thymine (T), cytosine (C), uracil (U), and guanine (G). As used herein, a modified nucleobase is a group of atoms capable of pairing with at least one unmodified nucleobase. A universal base is a nucleobase that can pair with any one of the five unmodified nucleobases. 
     As used herein, “nucleobase sequence” means the order of contiguous nucleobases in a nucleic acid or oligonucleotide independent of any sugar or internucleoside linkage modification. 
     As used herein, “nucleoside” means a moiety comprising a nucleobase and a sugar moiety. The nucleobase and sugar moiety are each, independently, unmodified or modified. As used herein, “modified nucleoside” means a nucleoside comprising a modified nucleobase and/or a modified sugar moiety. 
     As used herein, “oligomeric compound” means a compound consisting of an oligonucleotide and optionally one or more additional features, such as a conjugate group or terminal group. 
     As used herein, “oligonucleotide” means a strand of linked nucleosides connected via internucleoside linkages, wherein each nucleoside and internucleoside linkage may be modified or unmodified. Unless otherwise indicated, oligonucleotides consist of 8-50 linked nucleosides. As used herein, “modified oligonucleotide” means an oligonucleotide, wherein at least one nucleoside or internucleoside linkage is modified. As used herein, “unmodified oligonucleotide” means an oligonucleotide that does not comprise any nucleoside modifications or internucleoside modifications. 
     As used herein, “pharmaceutically acceptable carrier or diluent” means any substance suitable for use in administering to an animal. Certain such carriers enable pharmaceutical compositions to be formulated as, for example, liquids, powders, or suspensions that can be aerosolized or otherwise dispersed for inhalation by a subject. In certain embodiments, a pharmaceutically acceptable carrier or diluent is sterile water; sterile saline; or sterile buffer solution. 
     As used herein “pharmaceutically acceptable salts” means physiologically and pharmaceutically acceptable salts of compounds, such as oligomeric compounds, i.e., salts that retain the desired biological activity of the parent compound and do not impart undesired toxicological effects thereto. 
     As used herein “pharmaceutical composition” means a mixture of substances suitable for administering to a subject. For example, a pharmaceutical composition may comprise an antisense compound and an aqueous solution. 
     As used herein, “phosphorus moiety” means a group of atoms comprising a phosphorus atom. In certain embodiments, a phosphorus moiety comprises a mono-, di-, or tri-phosphate, or phosphorothioate. 
     As used herein “prodrug” means a therapeutic agent in a form outside the body that is converted to a different form within the body or cells thereof. Typically conversion of a prodrug within the body is facilitated by the action of an enzymes (e.g., endogenous or viral enzyme) or chemicals present in cells or tissues and/or by physiologic conditions. 
     As used herein, “RNAi compound” means an antisense compound that acts, at least in part, through RISC or Ago2 to modulate a target nucleic acid and/or protein encoded by a target nucleic acid. RNAi compounds include, but are not limited to double-stranded siRNA, single-stranded RNA (ssRNA), and microRNA, including microRNA mimics. In certain embodiments, an RNAi compound modulates the amount, activity, and/or splicing of a target nucleic acid. The term RNAi compound excludes antisense oligonucleotides that act through RNase H. 
     As used herein, the term “single-stranded” in reference to an antisense compound means such a compound consisting of one oligomeric compound that is not paired with a second oligomeric compound to form a duplex. “Self-complementary” in reference to an oligonucleotide means an oligonucleotide that at least partially hybridizes to itself. A compound consisting of one oligomeric compound, wherein the oligonucleotide of the oligomeric compound is self-complementary, is a single-stranded compound. A single-stranded antisense or oligomeric compound may be capable of binding to a complementary oligomeric compound to form a duplex, in which case the compound would no longer be single-stranded. 
     As used herein, “standard cell assay” means the assay described in Example 3 and reasonable variations thereof. 
     As used herein, “standard in vivo experiment” means the procedure described in Example 4, 6, or 7, and reasonable variations thereof. 
     As used herein, “stereorandom chiral center” in the context of a population of molecules of identical molecular formula means a chiral center having a random stereochemical configuration. For example, in a population of molecules comprising a stereorandom chiral center, the number of molecules having the (S) configuration of the stereorandom chiral center may be but is not necessarily the same as the number of molecules having the (R) configuration of the stereorandom chiral center. The stereochemical configuration of a chiral center is considered random when it is the result of a synthetic method that is not designed to control the stereochemical configuration. In certain embodiments, a stereorandom chiral center is a stereorandom phosphorothioate internucleoside linkage. 
     As used herein, “sugar moiety” means an unmodified sugar moiety or a modified sugar moiety. As used herein, “unmodified sugar moiety” means a 2′-OH(H) ribosyl moiety, as found in RNA (an “unmodified RNA sugar moiety”), or a 2′-H(H) moiety, as found in DNA (an “unmodified DNA sugar moiety”). As used herein, “modified sugar moiety” or “modified sugar” means a modified furanosyl sugar moiety or a sugar surrogate. As used herein, modified furanosyl sugar moiety means a furanosyl sugar comprising a non-hydrogen substituent in place of at least one hydrogen of an unmodified sugar moiety. In certain embodiments, a modified furanosyl sugar moiety is a 2′-substituted sugar moiety. Such modified furanosyl sugar moieties include bicyclic sugars and non-bicyclic sugars. As used herein, “sugar surrogate” means a modified sugar moiety having other than a furanosyl moiety that can link a nucleobase to another group, such as an internucleoside linkage, conjugate group, or terminal group in an oligonucleotide. Modified nucleosides comprising sugar surrogates can be incorporated into one or more positions within an oligonucleotide and such oligonucleotides are capable of hybridizing to complementary oligomeric compounds or nucleic acids. 
     As used herein, “target nucleic acid,” “target RNA,” “target RNA transcript” and “nucleic acid target” mean a nucleic acid that an antisense compound is designed to affect. 
     As used herein, “target region” means a portion of a target nucleic acid to which an antisense compound is designed to hybridize. 
     As used herein, “terminal group” means a chemical group or group of atoms that is covalently linked to a terminus of an oligonucleotide. 
     As used herein, “terminal wing nucleoside” means a nucleoside that is located at the terminus of a wing segment of a gapmer. Any wing segment that comprises or consists of at least two nucleosides has two termini: one that is immediately adjacent to the gap segment; and one that is at the end opposite the gap segment. Thus, any wing segment that comprises or consists of at least two nucleosides has two terminal nucleosides, one at each terminus. 
     As used herein, “therapeutically effective amount” means an amount of a compound, pharmaceutical agent, or composition that provides a therapeutic benefit to an individual. 
     As used herein, “treat” refers to administering a compound or pharmaceutical composition to an animal in order to effect an alteration or improvement of a disease, disorder, or condition in the animal. 
     CERTAIN EMBODIMENTS 
     Certain embodiments provide methods, compounds and compositions for inhibiting ENaC expression. 
     Certain embodiments provide compounds comprising or consisting of oligonucleotides complementary to an α-ENaC or SCNN1A nucleic acid. In certain embodiments, the α-ENaC or SCNN1A nucleic acid has the sequence set forth in RefSeq or GenBank Accession No. NM_001038.5 (disclosed herein as SEQ ID NO: 1), the complement of NC_000012.12 truncated from nucleosides 6343001 to 6380000 (disclosed herein as SEQ ID NO: 2), or NG_011945.1 (disclosed herein as SEQ ID NO: 1957). In certain embodiments, the compound is an antisense compound or oligomeric compound. In certain embodiments, the compound is single-stranded. In certain embodiments, the compound is double-stranded. 
     Certain embodiments provide a compound comprising a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound is an antisense compound or oligomeric compound. In certain embodiments, the compound is single-stranded. In certain embodiments, the compound is double-stranded. In certain embodiments, the modified oligonucleotide is 10 to 30 linked nucleosides in length. 
     Certain embodiments provide a compound comprising a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6 to 1954. For example, the nucleobase sequence of the modified oligonucleotide comprises or consists of any one of SEQ ID NOs 6, 7, etc. . . . or 1954. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 167. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 244. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 399. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 428. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 431. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 438. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 590. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 824. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 935. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1049. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1114. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1124. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1134. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1139. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1145. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1170. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1530. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1532. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1672. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1730. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1802. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1832. In certain embodiments, the compound is an antisense compound or oligomeric compound. In certain embodiments, the compound is single-stranded. In certain embodiments, the compound is double-stranded. In certain embodiments, the modified oligonucleotide is 10 to 30 linked nucleosides in length. In certain embodiments, the modified oligonucleotide has a nucleobase sequence comprising at least 12 contiguous nucleobases of any of SEQ ID Numbers from 6 to 1954. 
     Certain embodiments provide a compound comprising a modified oligonucleotide 10 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 10 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound is an antisense compound or oligomeric compound. In certain embodiments, the compound is single-stranded. In certain embodiments, the compound is double-stranded. In certain embodiments, the modified oligonucleotide is 10 to 30 linked nucleosides in length. 
     Certain embodiments provide a compound comprising a modified oligonucleotide 11 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 11 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound is an antisense compound or oligomeric compound. In certain embodiments, the compound is single-stranded. In certain embodiments, the compound is double-stranded. In certain embodiments, the modified oligonucleotide is 11 to 30 linked nucleosides in length. 
     Certain embodiments provide a compound comprising a modified oligonucleotide 12 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 12 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound is an antisense compound or oligomeric compound. In certain embodiments, the compound is single-stranded. In certain embodiments, the compound is double-stranded. In certain embodiments, the modified oligonucleotide is 12 to 30 linked nucleosides in length. 
     In certain embodiments, the compound comprises a modified oligonucleotide 30 linked nucleosides in length. In certain embodiments, the compound is an antisense compound or oligomeric compound. 
     Certain embodiments provide a compound comprising a modified oligonucleotide 16 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound is an antisense compound or oligomeric compound. In certain embodiments, the compound is single-stranded. In certain embodiments, the compound is double-stranded. In certain embodiments, the modified oligonucleotide is 16 to 30 linked nucleosides in length. 
     Certain embodiments provide a compound comprising a modified oligonucleotide consisting of the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound is an antisense compound or oligomeric compound. In certain embodiments, the compound is single-stranded. In certain embodiments, the compound is double-stranded. 
     In certain embodiments, compounds comprise or consist of modified oligonucleotides complementary to an intron of an α-ENaC nucleic acid transcript. In certain embodiments, modified oligonucleotides are complementary to intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain such embodiments, modified oligonucleotides are complementary to a sequence within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, compounds comprise or consist of oligonucleotides having at least an 8, 9, 10, 11, 12, 13, 14, 15, or 16 contiguous nucleobase portion complementary to an equal length portion of intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain embodiments, such oligonucleotides have at least an 8, 9, 10, 11, 12, 13, 14, 15, or 16 contiguous nucleobase portion complementary to an equal length portion within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, these compounds are antisense compounds or oligomeric compounds. Compounds comprising modified oligonucleotide complementary to nearly any portion of certain introns of an α-ENaC nucleic acid transcript, e.g., intron 4 of an α-ENaC pre-mRNA, are generally especially potent and tolerable. Thus, such certain introns can be considered hot spot regions for targeting an α-ENaC nucleic acid transcript. 
     In certain embodiments, compounds comprise or consist of modified oligonucleotides complementary to intron 4 or the 3′-UTR of an α-ENaC nucleic acid transcript. In certain embodiments, modified oligonucleotides are complementary to a sequence within nucleotides 17,951-24,120; or 32,129-33,174 of SEQ ID NO: 2. In certain embodiments, compounds comprise or consist of oligonucleotides having at least an 8, 9, 10, 11, 12, 13, 14, 15, or 16 contiguous nucleobase portion complementary to an equal length portion of intron 4 or the 3′-UTR of an α-ENaC nucleic acid transcript. In certain embodiments, such oligonucleotides have at least an 8, 9, 10, 11, 12, 13, 14, 15, or 16 contiguous nucleobase portion complementary to an equal length portion within nucleotides 17,951-24,120; or 32,129-33,174 of SEQ ID NO: 2. In certain embodiments, these compounds are antisense compounds or oligomeric compounds. 
     In certain embodiments, a compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having at least an 8, 9, 10, 11, 12, 13, 14, 15, or 16 contiguous nucleobase portion complementary to an equal length portion within nucleotides 19,022-19,037; 20,415-20,430; 21,750-21,766; 32,844-32,859; or 32,989-33,004 of SEQ ID NO: 2. In certain embodiments, the modified oligonucleotide is 10 to 30 linked nucleosides in length. 
     In certain embodiments, a compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and complementary within nucleotides 19,022-19,037; 20,415-20,430; 21,750-21,766; 32,844-32,859; or 32,989-33,004 of SEQ ID NO: 2. In certain embodiments, the modified oligonucleotide is 10 to 30 linked nucleosides in length. 
     In certain embodiments, a compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least an 8, 9, 10, 11, 12, 13, 14, 15, or 16 contiguous nucleobase portion of the nucleobase sequence of any one of compound numbers 797308, 797495, 826763, 827307, 827359, or 827392 (SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593). In certain embodiments, the modified oligonucleotide is 10 to 30 linked nucleosides in length. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 239. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 426. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1541. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1812. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 1113. In certain embodiments, the nucleobase sequence of the modified oligonucleotide comprises or consists of SEQ ID NO: 593. 
     In certain embodiments, a compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of compound numbers 797308, 797495, 826763, 827307, 827359, or 827392 (SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593). In certain embodiments, the modified oligonucleotide is 10 to 30 linked nucleosides in length. 
     In certain embodiments, a compound comprises a modified oligonucleotide having a nucleobase sequence consisting of the nucleobase sequence of any one of compound numbers 797308, 797495, 826763, 827307, 827359, or 827392 (SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593). 
     In certain embodiments, a compound comprising or consisting of a modified oligonucleotide complementary to α-ENaC is compound number 827359. Out of over 1,900 compounds that were screened as described in the Examples section below, compound numbers 797308, 797495, 826763, 827307, 827359, and 827392 emerged as the top lead compounds. In particular, compound number 827359 exhibited the best combination of properties in terms of potency and tolerability out of over 1,900 compounds. 
     Any of the foregoing oligonucleotides is a modified oligonucleotide comprising at least one modified internucleoside linkage, at least one modified sugar, and/or at least one modified nucleobase. 
     In certain embodiments, any of the foregoing modified oligonucleotides comprises at least one modified sugar. In certain embodiments, at least one modified sugar comprises a 2′-MOE modification. In certain embodiments, at least one modified sugar is a bicyclic sugar, such as a cEt bicyclic sugar, an LNA bicyclic sugar, or an ENA bicyclic sugar. 
     In certain embodiments, the modified oligonucleotide comprises at least one modified internucleoside linkage, such as a phosphorothioate internucleoside linkage. 
     In certain embodiments, any of the foregoing modified oligonucleotides comprises at least one modified nucleobase, such as 5-methylcytosine. 
     In certain embodiments, any of the foregoing modified oligonucleotides comprises:
         a gap segment consisting of linked 2′-deoxynucleosides;   a 5′ wing segment consisting of linked nucleosides; and   a 3′ wing segment consisting of linked nucleosides;       

     wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment and wherein each nucleoside of each wing segment comprises a modified sugar. In certain embodiments, the modified oligonucleotide is 16 to 50 linked nucleosides in length having a nucleobase sequence comprising the sequence recited in any one of SEQ ID NO: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the modified oligonucleotide is 10 to 30 linked nucleosides in length having a nucleobase sequence comprising the sequence recited in any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the modified oligonucleotide is 16 linked nucleosides in length having a nucleobase sequence consisting of the sequence recited in any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. 
     In certain embodiments, a compound comprises or consists of a modified oligonucleotide 20-80 linked nucleobases in length having a nucleobase sequence comprising the sequence recited in any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593, wherein the modified oligonucleotide comprises 
     a gap segment consisting of ten linked 2′-deoxynucleosides; 
     a 5′ wing segment consisting of five linked nucleosides; and 
     a 3′ wing segment consisting of five linked nucleosides; 
     wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment, wherein each nucleoside of each wing segment comprises a 2′-O-methoxyethyl sugar; wherein each internucleoside linkage is a phosphorothioate linkage and wherein each cytosine is a 5-methylcytosine. In certain embodiments, the modified oligonucleotide consists of 20-30 linked nucleosides. In certain embodiments, the modified oligonucleotide consists of 20 linked nucleosides. 
     In certain embodiments, a compound comprises or consists of a modified oligonucleotide 16-80 linked nucleobases in length having a nucleobase sequence comprising the sequence recited in any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593, wherein the modified oligonucleotide comprises 
     a gap segment consisting of ten linked 2′-deoxynucleosides; 
     a 5′ wing segment consisting of three linked nucleosides; and 
     a 3′ wing segment consisting of three linked nucleosides; 
     wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment; wherein the nucleosides of the 5′ wing segment each comprise a cEt bicyclic sugar; wherein the nucleosides of the 3′ wing segment each comprises a cEt bicyclic sugar; wherein each internucleoside linkage is a phosphorothioate linkage; and wherein each cytosine is a 5-methylcytosine. In certain embodiments, the modified oligonucleotide is 16-80 linked nucleosides in length. In certain embodiments, the modified oligonucleotide is 16-30 linked nucleosides in length. 
     In certain embodiments, a compound comprises or consists of a modified oligonucleotide according to one of the following formulas: 
       mCks mCks mCks Gds Ads Tds Ads Gds mCds Tds Gds Gds Tds Tks Gks Tk  (SEQ ID NO: 1113);
 
       Aks Aks Gks Tds Ads Tds Gds Gds Tds Gds mCds Ads Ads mCks Aks Gk  (SEQ ID NO: 239);
 
       Aks mCks Gks Ads Tds Tds Ads mCds Ads Gds Gds Gds Ads Tks Tks mCk  (SEQ ID NO: 426);
 
       Tks Gks mCks Ads Tds Ads Gds Gds Ads Gds Tds Tds mCds Tks mCks Tk  (SEQ ID NO: 1541);
 
       Aks Gks Aks Gds Tds Ads Ads Tds Gds Ads Ads Ads mCds mCks mCks Ak  (SEQ ID NO: 1812);
 
       mCks Gks Aks Tds Tds Ads mCds Ads Gds Gds Gds Ads Tds Tks mCks Ak  (SEQ ID NO: 593);
 
     wherein A=an adenine, mC=a 5-methylcytosine, G=a guanine, T=a thymine, k=a cEt sugar moiety, d=a 2′-deoxyribosyl sugar moiety, and s=a phosphorothioate internucleoside linkage. 
     In certain embodiments, a compound comprises or consists of compound 827359 or salt thereof, a modified oligonucleotide having the following chemical structure: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a compound comprises or consists of the sodium salt of compound 827359, having the following chemical structure: 
     
       
         
         
             
             
         
       
     
     In any of the foregoing embodiments, the compound or oligonucleotide can be at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% complementary to a nucleic acid encoding α-ENaC. 
     In any of the foregoing embodiments, the compound can be single-stranded. In certain embodiments, the compound comprises 2′-deoxyribonucleosides. In certain embodiments, the compound is double-stranded. In certain embodiments, the compound is double-stranded and comprises ribonucleosides. In any of the foregoing embodiments, the compound can be an antisense compound or oligomeric compound. 
     In any of the foregoing embodiments, the compound can be 8 to 80, 10 to 30, 12 to 50, 13 to 30, 13 to 50, 14 to 30, 14 to 50, 15 to 30, 15 to 50, 16 to 30, 16 to 50, 17 to 30, 17 to 50, 18 to 22, 18 to 24, 18 to 30, 18 to 50, 19 to 22, 19 to 30, 19 to 50, or 20 to 30 linked nucleosides in length. In certain embodiments, the compound comprises or consists of an oligonucleotide. 
     In certain embodiments, a compound comprises a modified oligonucleotide described herein and a conjugate group. In certain embodiments, the conjugate group is linked to the modified oligonucleotide at the 5′ end of the modified oligonucleotide. In certain embodiments, the conjugate group is linked to the modified oligonucleotide at the 3′ end of the modified oligonucleotide. 
     In certain embodiments, compounds or compositions provided herein comprise a salt of the modified oligonucleotide. In certain embodiments, the salt is a sodium salt. In certain embodiments, the salt is a potassium salt. 
     In certain embodiments, the compounds or compositions as described herein are active by virtue of having at least one of an in vitro IC 50  of less than 250 nM, less than 200 nM, less than 150 nM, less than 100 nM, less than 90 nM, less than 80 nM, less than 70 nM, less than 65 nM, less than 60 nM, less than 55 nM, less than 50 nM, less than 45 nM, less than 40 nM, less than 35 nM, less than 30 nM, less than 25 nM, less than 20 nM, or less than 15 nM in a standard cell assay. 
     In certain embodiments, the compounds or compositions as described herein are highly tolerable as demonstrated by having at least one of an increase an alanine transaminase (ALT) or aspartate transaminase (AST) value of no more than 4 fold, 3 fold, 2 fold, or 1.5 fold over saline treated animals or an increase in liver, spleen, or kidney weight of no more than 30%, 20%, 15%, 12%, 10%, 5%, or 2% compared to control treated animals. In certain embodiments, the compounds or compositions as described herein are highly tolerable as demonstrated by having no increase of ALT or AST over control treated animals. In certain embodiments, the compounds or compositions as described herein are highly tolerable as demonstrated by having no increase in liver, spleen, or kidney weight over control animals. 
     Certain embodiments provide a composition comprising the compound of any of the aforementioned embodiments or salt thereof and at least one of a pharmaceutically acceptable carrier or diluent. In certain embodiments, the composition has a viscosity less than about 40 centipoise (cP), less than about 30 cP, less than about 20 cP, less than about 15 cP, less than about 10 cP, less than about 5 cP, or less than about 3 cP, or less than about 1.5 cP. In certain embodiments, the composition having any of the aforementioned viscosities comprises a compound provided herein at a concentration of about 15 mg/mL, 20 mg/mL, 25 mg/mL, or about 50 mg/mL. In certain embodiments, the composition having any of the aforementioned viscosities and/or compound concentrations has a temperature of room temperature or about 20° C., about 21° C., about 22° C., about 23° C., about 24° C., about 25° C., about 26° C., about 27° C., about 28° C., about 29° C., or about 30° C. 
     Any of the foregoing compounds can be used for treating, preventing, or ameliorating a disease associated with ENaC as further described herein. 
     Certain Indications 
     Certain embodiments provided herein relate to methods of inhibiting ENaC expression, which can be useful for treating, preventing, or ameliorating a disease associated with ENaC in an individual, by administration of a compound that targets α-ENaC. In certain embodiments, the compound can be an α-ENaC inhibitor. In certain embodiments, the compound can be an antisense compound, oligomeric compound, or oligonucleotide complementary to α-ENaC. In certain embodiments, the compound can be any of the compounds described herein. 
     Examples of diseases associated with ENaC that are treatable, preventable, and/or ameliorable with the methods provided herein include cystic fibrosis, COPD, asthma, and chronic bronchitis. 
     In certain embodiments, a method of treating, preventing, or ameliorating a disease associated with α-ENaC in an individual comprises administering to the individual a compound comprising an α-ENaC inhibitor, thereby treating, preventing, or ameliorating the disease. In certain embodiments, the compound comprises an antisense compound targeted to α-ENaC. In certain embodiments, the compound comprises an oligonucleotide complementary to an α-ENaC nucleic acid transcript. In certain embodiments, the oligonucleotide is a modified oligonucleotide. In certain embodiments, the compound comprise a modified oligonucleotide complementary to an intron of an α-ENaC nucleic acid transcript. In certain embodiments, the modified oligonucleotide is complementary to intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain such embodiments, the oligonucleotide is complementary to a sequence within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, the compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 12 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide consisting of the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 16 to 50 linked nucleosides in length having a nucleobase sequence comprising any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the compound comprises a modified oligonucleotide having a nucleobase sequence consisting of any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In any of the foregoing embodiments, the modified oligonucleotide can be 10 to 30 linked nucleosides in length. In certain embodiments, the compound is compound number 797308, 797495, 826763, 827307, 827359, or 827392. In any of the foregoing embodiments, the compound can be single-stranded or double-stranded. In any of the foregoing embodiments, the compound can be an antisense compound or oligomeric compound. In certain embodiments, the compound is administered to the individual via inhalation. In certain embodiments, administering the compound improves or preserves spirometry or mucociliary clearance. 
     In certain embodiments, a method of treating, preventing, or ameliorating cystic fibrosis, COPD, asthma, or chronic bronchitis comprises administering to the individual a compound comprising a modified oligonucleotide complementary to an α-ENaC nucleic acid, thereby treating, preventing, or ameliorating cystic fibrosis, COPD, asthma, or chronic bronchitis. In certain embodiments, the compound is an antisense compound targeted to α-ENaC. In certain embodiments, the oligonucleotide is a modified oligonucleotide. In certain embodiments, the compound comprise a modified oligonucleotide complementary to an intron of an α-ENaC nucleic acid transcript. In certain embodiments, the modified oligonucleotide is complementary to intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain such embodiments, the oligonucleotide is complementary to a sequence within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, the compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 12 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide consisting of the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide of 16 to 50 linked nucleosides in length having a nucleobase sequence comprising any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the compound comprises a modified oligonucleotide having a nucleobase sequence consisting of any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In any of the foregoing embodiments, the modified oligonucleotide can be 10 to 30 linked nucleosides in length. In certain embodiments, the compound is compound number 797308, 797495, 826763, 827307, 827359, or 827392. In any of the foregoing embodiments, the compound can be single-stranded or double-stranded. In any of the foregoing embodiments, the compound can be an antisense compound or oligomeric compound. In certain embodiments, the compound is administered to the individual via inhalation. In certain embodiments, administering the compound improves or preserves lung function. In certain such embodiments, spirometry or mucociliary clearance is improved or preserved. In certain such embodiments, forced expiratory volume in one second (FEV 1 ), FVC, or FEF 25-75  is increased. In certain embodiments, pulmonary exacerbations, hospitalization rate or frequency, or antibiotic use is decreased. In certain embodiments, quality of life is improved, as measured by the respiratory questionnaire, CFQ-R. In certain embodiments, the individual is identified as having or at risk of having a disease associated with ENaC. 
     In certain embodiments, a method of inhibiting expression of α-ENaC in an individual having, or at risk of having, a disease associated with ENaC comprises administering to the individual a compound comprising an α-ENaC inhibitor, thereby inhibiting expression of α-ENaC in the individual. In certain embodiments, administering the compound inhibits expression of α-ENaC in the lung. In certain embodiments, the individual has, or is at risk of having cystic fibrosis, COPD, asthma, or chronic bronchitis. In certain embodiments, the compound comprises an antisense compound targeted to α-ENaC. In certain embodiments, the compound comprises an oligonucleotide complementary to an α-ENaC nucleic acid transcript. In certain embodiments, the oligonucleotide is a modified oligonucleotide. In certain embodiments, the compound comprise a modified oligonucleotide complementary to an intron of an α-ENaC nucleic acid transcript. In certain embodiments, the modified oligonucleotide is complementary to intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain such embodiments, the oligonucleotide is complementary to a sequence within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, the compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 12 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide consisting of the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 16 to 50 linked nucleosides in length having a nucleobase sequence comprising any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the compound comprises a modified oligonucleotide having a nucleobase sequence consisting of any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In any of the foregoing embodiments, the modified oligonucleotide can be 10 to 30 linked nucleosides in length. In certain embodiments, the compound is compound number 797308, 797495, 826763, 827307, 827359, or 827392. In any of the foregoing embodiments, the compound can be single-stranded or double-stranded. In any of the foregoing embodiments, the compound can be an antisense compound or oligomeric compound. In certain embodiments, the compound is administered to the individual via inhalation. In certain embodiments, administering the compound improves or preserves spirometry or mucociliary clearance. In certain embodiments, the individual is identified as having or at risk of having a disease associated with ENaC. 
     In certain embodiments, a method of inhibiting expression of α-ENaC in a cell comprises contacting the cell with a compound comprising an α-ENaC inhibitor, thereby inhibiting expression of α-ENaC in the cell. In certain embodiments, the cell is a lung cell. In certain embodiments, the cell is in the lung. In certain embodiments, the cell is in the lung of an individual who has, or is at risk of having cystic fibrosis, COPD, asthma, or chronic bronchitis. In certain embodiments, the compound comprises an antisense compound targeted to α-ENaC. In certain embodiments, the compound comprises an oligonucleotide complementary to an α-ENaC nucleic acid transcript. In certain embodiments, the oligonucleotide is a modified oligonucleotide. In certain embodiments, the compound comprise a modified oligonucleotide complementary to an intron of an α-ENaC nucleic acid transcript. In certain embodiments, the modified oligonucleotide is complementary to intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain such embodiments, the oligonucleotide is complementary to a sequence within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, the compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 12 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide consisting of the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 16 to 50 linked nucleosides in length having a nucleobase sequence comprising any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the compound comprises a modified oligonucleotide having a nucleobase sequence consisting of any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In any of the foregoing embodiments, the modified oligonucleotide can be 10 to 30 linked nucleosides in length. In certain embodiments, the compound is compound number 797308, 797495, 826763, 827307, 827359, or 827392. In any of the foregoing embodiments, the compound can be single-stranded or double-stranded. In any of the foregoing embodiments, the compound can be an antisense compound or oligomeric compound. 
     In certain embodiments, a method of increasing or improving spirometry or mucociliary clearance in the lung of an individual having, or at risk of having, a disease associated with ENaC comprises administering to the individual a compound comprising an α-ENaC inhibitor, thereby increasing or improving spirometry or mucociliary clearance in the lung of the individual. In certain such embodiments, forced expiratory volume in one second (FEV 1 ), FVC, or FEF 25-75  is increased. In certain embodiments, pulmonary exacerbations, hospitalization rate or frequency, or antibiotic use is decreased. In certain embodiments, quality of life is improved, as measured by the respiratory questionnaire, CFQ-R. In certain embodiments, the individual has, or is at risk of having, cystic fibrosis, COPD, asthma, or chronic bronchitis. In certain embodiments, the compound comprises an antisense compound targeted to α-ENaC. In certain embodiments, the compound comprises an oligonucleotide complementary to an α-ENaC nucleic acid transcript. In certain embodiments, the oligonucleotide is a modified oligonucleotide. In certain embodiments, the compound comprise a modified oligonucleotide complementary to an intron of an α-ENaC nucleic acid transcript. In certain embodiments, the modified oligonucleotide is complementary to intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain such embodiments, the oligonucleotide is complementary to a sequence within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, the compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 12 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide consisting of the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 16 to 50 linked nucleosides in length having a nucleobase sequence comprising any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the compound comprises a modified oligonucleotide having a nucleobase sequence consisting of any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In any of the foregoing embodiments, the modified oligonucleotide can be 10 to 30 linked nucleosides in length. In certain embodiments, the compound is compound number 797308, 797495, 826763, 827307, 827359, or 827392. In any of the foregoing embodiments, the compound can be single-stranded or double-stranded. In any of the foregoing embodiments, the compound can be an antisense compound or oligomeric compound. In certain embodiments, the compound is administered to the individual via inhalation. In certain embodiments, the individual is identified as having or at risk of having a disease associated with ENaC. 
     Certain embodiments are drawn to a compound comprising an α-ENaC inhibitor for use in treating a disease associated with ENaC. In certain embodiments, the disease is cystic fibrosis, COPD, asthma, or chronic bronchitis. In certain embodiments, the compound comprises an antisense compound targeted to α-ENaC. In certain embodiments, the compound comprises an oligonucleotide complementary to an α-ENaC nucleic acid transcript. In certain embodiments, the oligonucleotide is a modified oligonucleotide. In certain embodiments, the compound comprise a modified oligonucleotide complementary to an intron of an α-ENaC nucleic acid transcript. In certain embodiments, the modified oligonucleotide is complementary to intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain such embodiments, the oligonucleotide is complementary to a sequence within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, the compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 12 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide consisting of the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 16 to 50 linked nucleosides in length having a nucleobase sequence comprising any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the compound comprises a modified oligonucleotide having a nucleobase sequence consisting of any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In any of the foregoing embodiments, the modified oligonucleotide can be 10 to 30 linked nucleosides in length. In certain embodiments, the compound is compound number 797308, 797495, 826763, 827307, 827359, or 827392. In any of the foregoing embodiments, the compound can be single-stranded or double-stranded. In any of the foregoing embodiments, the compound can be an antisense compound or oligomeric compound. 
     Certain embodiments are drawn to a compound comprising an α-ENaC inhibitor for use in increasing or improving spirometry or mucociliary clearance of an individual having or at risk of having cystic fibrosis, COPD, asthma, or chronic bronchitis. In certain embodiments, the compound comprises an antisense compound targeted to α-ENaC. In certain embodiments, the compound comprises an oligonucleotide complementary to an α-ENaC nucleic acid transcript. In certain embodiments, the oligonucleotide is a modified oligonucleotide. In certain embodiments, the compound comprise a modified oligonucleotide complementary to an intron of an α-ENaC nucleic acid transcript. In certain embodiments, the modified oligonucleotide is complementary to intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain such embodiments, the oligonucleotide is complementary to a sequence within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, the compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 12 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide consisting of the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 16 to 50 linked nucleosides in length having a nucleobase sequence comprising any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the compound comprises a modified oligonucleotide having a nucleobase sequence consisting of any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In any of the foregoing embodiments, the modified oligonucleotide can be 10 to 30 linked nucleosides in length. In certain embodiments, the compound is compound number 797308, 797495, 826763, 827307, 827359, or 827392. In any of the foregoing embodiments, the compound can be single-stranded or double-stranded. In any of the foregoing embodiments, the compound can be an antisense compound or oligomeric compound. 
     Certain embodiments are drawn to use of a compound comprising an α-ENaC inhibitor for the manufacture or preparation of a medicament for treating a disease associated with ENaC. Certain embodiments are drawn to use of a compound comprising an α-ENaC inhibitor for the preparation of a medicament for treating a disease associated with ENaC. In certain embodiments, the disease is cystic fibrosis, COPD, asthma, or chronic bronchitis. In certain embodiments, the compound comprises an antisense compound targeted to α-ENaC. In certain embodiments, the compound comprises an oligonucleotide complementary to an α-ENaC nucleic acid transcript. In certain embodiments, the oligonucleotide is a modified oligonucleotide. In certain embodiments, the compound comprise a modified oligonucleotide complementary to an intron of an α-ENaC nucleic acid transcript. In certain embodiments, the modified oligonucleotide is complementary to intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain such embodiments, the oligonucleotide is complementary to a sequence within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, the compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 12 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide consisting of the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 16 to 50 linked nucleosides in length having a nucleobase sequence comprising any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the compound comprises a modified oligonucleotide having a nucleobase sequence consisting of any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In any of the foregoing embodiments, the modified oligonucleotide can be 10 to 30 linked nucleosides in length. In certain embodiments, the compound is compound number 797308, 797495, 826763, 827307, 827359, or 827392. In any of the foregoing embodiments, the compound can be single-stranded or double-stranded. In any of the foregoing embodiments, the compound can be an antisense compound or oligomeric compound. 
     Certain embodiments are drawn to use of a compound comprising an α-ENaC inhibitor for the manufacture or preparation of a medicament for increasing or improving spirometry or mucociliary clearance in an individual having or at risk of having cystic fibrosis, COPD, asthma, or chronic bronchitis. In certain embodiments, the compound comprises an antisense compound targeted to α-ENaC. In certain embodiments, the compound comprises an oligonucleotide complementary to an α-ENaC nucleic acid transcript. In certain embodiments, the oligonucleotide is a modified oligonucleotide. In certain embodiments, the compound comprise a modified oligonucleotide complementary to an intron of an α-ENaC nucleic acid transcript. In certain embodiments, the modified oligonucleotide is complementary to intron 1, intron 2, intron 3, intron 4, intron 5, intron 6, intron 7, intron 8, intron 9, intron 10, intron 11, or intron 12 of an α-ENaC nucleic acid transcript. In certain such embodiments, the oligonucleotide is complementary to a sequence within nucleotides 4,497-5,163; 5,634-16,290; 16,559-17,759; 17,951-24,120; 24,225-24,565; 24,730-25,152; 25,252-25,445; 25,564-30,595; 30,675-30,779; 30,838-30,995; 31,052-31,198; or 31,275-31,747 of SEQ ID NO: 2. In certain embodiments, the compound comprises a modified oligonucleotide 8 to 50 linked nucleosides in length and having a nucleobase sequence comprising at least 8 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 12 to 50 linked nucleosides in length and having a nucleobase sequence comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide consisting of the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a modified oligonucleotide 16 to 50 linked nucleosides in length having a nucleobase sequence comprising any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In certain embodiments, the compound comprises a modified oligonucleotide having a nucleobase sequence consisting of any one of SEQ ID NOs: 239, 426, 1541, 1812, 1113, or 593. In any of the foregoing embodiments, the modified oligonucleotide can be 10 to 30 linked nucleosides in length. In certain embodiments, the compound is compound number 797308, 797495, 826763, 827307, 827359, or 827392. In any of the foregoing embodiments, the compound can be single-stranded or double-stranded. In any of the foregoing embodiments, the compound can be an antisense compound or oligomeric compound. 
     In any of the foregoing methods or uses, the compound can be targeted to α-ENaC. In certain embodiments, the compound comprises or consists of a modified oligonucleotide, for example a modified oligonucleotide 8 to 50 linked nucleosides in length, 10 to 30 linked nucleosides in length, 12 to 30 linked nucleosides in length, or 20 linked nucleosides in length. In certain embodiments, the modified oligonucleotide is at least 80%, 85%, 90%, 95% or 100% complementary to any of the nucleobase sequences recited in SEQ ID NOs: 1, 2, or 1957. In certain embodiments, the modified oligonucleotide comprises at least one modified internucleoside linkage, at least one modified sugar, and at least one modified nucleobase. In certain such embodiments, the atleast one modified internucleoside linkage is a phosphorothioate internucleoside linkage, the at least one modified sugar is a bicyclic sugar or a 2′-MOE sugar, and the at least one modified nucleobase is a 5-methylcytosine. In certain embodiments, the modified oligonucleotide comprises a gap segment consisting of linked 2′-deoxynucleosides; a 5′ wing segment consisting of linked nucleosides; and a 3′ wing segment consisting of linked nucleosides, wherein the gap segment is positioned immediately adjacent to and between the 5′ wing segment and the 3′ wing segment and wherein each terminal wing nucleoside comprises a modified sugar. 
     In any of the foregoing embodiments, the modified oligonucleotide is 12 to 30, 15 to 30, 15 to 25, 15 to 24, 16 to 24, 17 to 24, 18 to 24, 19 to 24, 20 to 24, 19 to 22, 20 to 22, 16 to 20, or 17 or 20 linked nucleosides in length. In certain embodiments, the modified oligonucleotide is at least 80%, 85%, 90%, 95% or 100% complementary to any of the nucleobase sequences recited in SEQ ID NOs: 1, 2, or 1957. In certain embodiments, the modified oligonucleotide comprises at least one modified internucleoside linkage, at least one modified sugar, and at least one modified nucleobase. In certain embodiments, the at least one modified internucleoside linkage is a phosphorothioate internucleoside linkage, the at least one modified sugar is a bicyclic sugar or a 2′-MOE sugar, and the at least one modified nucleobase is a 5-methylcytosine. In certain embodiments, the modified oligonucleotide comprises a gap segment consisting of linked 2′-deoxynucleosides; a 5′ wing segment consisting of linked nucleosides; and a 3′ wing segment consisting of linked nucleosides, wherein the gap segment is positioned immediately adjacent to and between the 5′ wing segment and the 3′ wing segment and wherein each terminal wing nucleoside comprises a modified sugar. 
     In any of the foregoing methods or uses, the compound comprises or consists of a modified oligonucleotide 16 to 30 linked nucleosides in length and having a nucleobase sequence comprising any one of SEQ ID NOs: 6-1954, wherein the modified oligonucleotide comprises:
         a gap segment consisting of linked 2′-deoxynucleosides;   a 5′ wing segment consisting of linked nucleosides; and   a 3′ wing segment consisting of linked nucleosides;       

     wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment and wherein each nucleoside of each wing segment comprises a modified sugar. 
     In any of the foregoing methods or uses, the compound comprises or consists of a modified oligonucleotide 16 to 30 linked nucleosides in length and having a nucleobase sequence comprising any one of SEQ ID NOs: 6-1954, wherein the modified oligonucleotide comprises:
         a gap segment consisting of linked 2′-deoxynucleosides;   a 5′ wing segment consisting of linked nucleosides; and   a 3′ wing segment consisting of linked nucleosides;       

     wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment, wherein each terminal wing nucleoside comprises a modified sugar. 
     In any of the foregoing methods or uses, the compound comprises or consists a modified oligonucleotide 20 linked nucleosides in length having a nucleobase sequence comprising the sequence recited in any one of SEQ ID NOs: 6-1954, wherein the modified oligonucleotide comprises 
     a gap segment consisting of ten linked 2′-deoxynucleosides; 
     a 5′ wing segment consisting of five linked nucleosides; and 
     a 3′ wing segment consisting of five linked nucleosides; 
     wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment, wherein each nucleoside of each wing segment comprises a 2′-O-methoxyethyl sugar; wherein each internucleoside linkage is a phosphorothioate linkage and wherein each cytosine is a 5-methylcytosine. In certain embodiments, the modified oligonucleotide consists of 20-30 linked nucleosides. In certain embodiments, the modified oligonucleotide consists of 20 linked nucleosides. 
     In any of the foregoing methods or uses, the compound comprises or consists a modified oligonucleotide 16 to 50 linked nucleobases in length having a nucleobase sequence comprising or consisting of the sequence recited in any one of SEQ ID NOs: 6-1954, wherein the modified oligonucleotide comprises 
     a gap segment consisting of 10 linked 2′-deoxynucleosides; 
     a 5′ wing segment consisting of 3 linked nucleosides; and 
     a 3′ wing segment consisting of 3 linked nucleosides; 
     wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment, wherein each nucleoside of each wing segment comprises a cEt sugar; wherein each internucleoside linkage is a phosphorothioate linkage and wherein each cytosine is a 5-methylcytosine. In certain embodiments, the modified oligonucleotide consists of 16-30 linked nucleosides. In certain embodiments, the modified oligonucleotide consists of 16 linked nucleosides. 
     In any of the foregoing methods or uses, the compound comprises or consists a modified oligonucleotide 16 to 50 linked nucleobases in length having a nucleobase sequence comprising or consisting of the sequence recited in any one of SEQ ID NOs: 239, 426, 593, 1113, 1541, or 1812, wherein the modified oligonucleotide comprises 
     a gap segment consisting of 10 linked 2′-deoxynucleosides; 
     a 5′ wing segment consisting of 3 linked nucleosides; and 
     a 3′ wing segment consisting of 3 linked nucleosides; 
     wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment, wherein each nucleoside of each wing segment comprises a cEt sugar; wherein each internucleoside linkage is a phosphorothioate linkage and wherein each cytosine is a 5-methylcytosine. In certain embodiments, the modified oligonucleotide consists of 16-30 linked nucleosides. In certain embodiments, the modified oligonucleotide consists of 16 linked nucleosides. 
     In any of the foregoing methods or uses, the compound has the following chemical structure: 
     
       
         
         
             
             
         
       
     
     In any of the foregoing methods or uses, the compound can be administered via inhalation. In certain embodiments, the compound of any of the foregoing methods or uses can be administered through injection or infusion. In certain embodiments, the compound of any of the foregoing methods or uses can be administered via subcutaneous administration, intravenous administration, intramuscular administration, intraarterial administration, intraperitoneal administration, or intracranial administration, e.g. intrathecal or intracerebroventricular administration. In certain embodiments, the compound of any of the foregoing methods or uses can be administered systemically. In certain embodiments, the compound of any of the foregoing methods or uses can be administered orally. 
     Certain Combinations and Combination Therapies 
     In certain embodiments, a first agent comprising the compound described herein is co-administered with one or more secondary agents. In certain embodiments, such second agents are designed to treat the same disease, disorder, or condition as the first agent described herein. In certain embodiments, such second agents are designed to treat a different disease, disorder, or condition as the first agent described herein. In certain embodiments, a first agent is designed to treat an undesired side effect of a second agent. In certain embodiments, second agents are co-administered with the first agent to treat an undesired effect of the first agent. In certain embodiments, such second agents are designed to treat an undesired side effect of one or more pharmaceutical compositions as described herein. In certain embodiments, second agents are co-administered with the first agent to produce a combinational effect. In certain embodiments, second agents are co-administered with the first agent to produce a synergistic effect. In certain embodiments, the co-administration of the first and second agents permits use of lower dosages than would be required to achieve a therapeutic or prophylactic effect if the agents were administered as independent therapy. 
     In certain embodiments, one or more compounds or compositions provided herein are co-administered with one or more secondary agents. In certain embodiments, one or more compounds or compositions provided herein and one or more secondary agents, are administered at different times. In certain embodiments, one or more compounds or compositions provided herein and one or more secondary agents, are prepared together in a single formulation. In certain embodiments, one or more compounds or compositions provided herein and one or more secondary agents, are prepared separately. In certain embodiments, a secondary agent is a bronchodilator, a corticosteroid, an antibiotic, a second compound comprising or consisting of a modified oligonucleotide, and/or a chloride channel (CFTR) modulator. In certain embodiments, a secondary agent is selected from: hypertonic saline, dornase alfa, ivacaftor, tezacaftor, and lumacaftor. 
     Certain embodiments are directed to the use of a compound comprising a modified oligonucleotide complementary to an α-ENaC nucleic acid transcript as described herein in combination with a secondary agent. In particular embodiments such use is in a method of treating a patient suffering from cystic fibrosis, COPD, asthma, or chronic bronchitis or in the preparation or manufacture of a medicament for treating cystic fibrosis, COPD, asthma, or chronic bronchitis. In certain embodiments, a secondary agent is a bronchodilator, a corticosteroid, an antibiotic, or a chloride channel (CFTR) modulator. In certain embodiments, a secondary agent is selected from: hypertonic saline, dornase alfa, ivacaftor, tezacaftor, and lumacaftor. 
     Certain embodiments are directed to the use of a compound comprising a modified oligonucleotide complementary to an α-ENaC nucleic acid transcript as described herein in combination with two or more secondary agents. In particular embodiments such use is in a method of treating a patient suffering from cystic fibrosis, COPD, asthma, or chronic bronchitis or in the preparation or manufacture of a medicament for treating cystic fibrosis, COPD, asthma, or chronic bronchitis. In certain embodiments, two or more secondary agents are selected from bronchodilators, corticosteroids, antibiotics, and chloride channel (CFTR) modulators. In certain embodiments, two or more secondary agents are selected from: hypertonic saline, dornase alfa, ivacaftor, tezacaftor, and lumacaftor. 
     Certain embodiments are drawn to a combination of a compound comprising a modified oligonucleotide complementary to an α-ENaC nucleic acid transcript as described herein and a secondary agent, such as a secondary agent selected from: hypertonic saline, dornase alfa, ivacaftor, tezacaftor, and lumacaftor. In certain embodiments, such a combination of a compound comprising a modified oligonucleotide complementary to an α-ENaC nucleic acid transcript as described herein and a secondary agent, such as a secondary agent selected from: hypertonic saline, dornase alfa, ivacaftor, tezacaftor, and lumacaftor is useful for improving or preserving spirometry or mucociliary clearance and/or treating cystic fibrosis, COPD, asthma, or chronic bronchitis. 
     Certain embodiments are drawn to a combination of a compound comprising a modified oligonucleotide complementary to an α-ENaC nucleic acid transcript as described herein and two or more secondary agents, such as secondary agents selected from: hypertonic saline, dornase alfa, ivacaftor, tezacaftor, and lumacaftor. In certain embodiments, such a combination of a compound comprising a modified oligonucleotide complementary to an α-ENaC nucleic acid transcript as described herein and two ore more secondary agents, such as secondary agents selected from: hypertonic saline, dornase alfa, ivacaftor, tezacaftor, and lumacaftor is useful for improving or preserving spirometry or mucociliary clearance and/or treating cystic fibrosis, COPD, asthma, or chronic bronchitis. 
     In certain embodiments the compound comprising a modified oligonucleotide complementary to an α-ENaC nucleic acid transcript as described herein and the secondary agent are used in combination treatment by administering the two agents simultaneously, separately or sequentially. In certain embodiments the two agents are formulated as a fixed dose combination product. In other embodiments the two agents are provided to the patient as separate units which can then either be taken simultaneously or serially (sequentially). 
     In certain embodiments the compound comprising a modified oligonucleotide complementary to an α-ENaC nucleic acid transcript as described herein and two or more secondary agents are used in combination treatment by administering the three or more agents simultaneously, separately or sequentially. In certain embodiments the three or more agents are formulated as a fixed dose combination product. In other embodiments the three or more agents are provided to the patient as separate units which can then either be taken simultaneously or serially (sequentially). 
     Certain Compounds 
     In certain embodiments, compounds described herein can be antisense compounds. In certain embodiments, the antisense compound comprises or consists of an oligomeric compound. In certain embodiments, the oligomeric compound comprises or consists of a modified oligonucleotide. In certain embodiments, the modified oligonucleotide has a nucleobase sequence complementary to that of a target nucleic acid. 
     In certain embodiments, a compound described herein comprises or consists of a modified oligonucleotide. In certain embodiments, the modified oligonucleotide has a nucleobase sequence complementary to that of a target nucleic acid. 
     In certain embodiments, a compound or antisense compound is single-stranded. Such a single-stranded compound or antisense compound comprises or consists of an oligomeric compound. In certain embodiments, such an oligomeric compound comprises or consists of an oligonucleotide and optionally a conjugate group. In certain embodiments, the oligonucleotide is an antisense oligonucleotide. In certain embodiments, the oligonucleotide is modified. In certain embodiments, the oligonucleotide of a single-stranded antisense compound or oligomeric compound comprises a self-complementary nucleobase sequence. 
     In certain embodiments, a compound or antisense compound is double-stranded. Such double-stranded compounds comprise a first oligomeric compound comprising or consisting of a first modified oligonucleotide having a region complementary to a target nucleic acid and a second oligomeric compound comprising or consisting of a second oligonucleotide having a region complementary to the first modified oligonucleotide. In certain embodiments, the first oligonucleotide is 100% complementary to the second oligonucleotide. In certain embodiments, the first and second oligonucleotides include non-complementary, overhanging nucleosides. In certain embodiments, the first modified oligonucleotide comprises unmodified ribosyl sugar moieties as those found in RNA. In such embodiments, thymine nucleobases in the first and/or second oligonucleotide are replaced by uracil nucleobases. In certain embodiments, the first and/or second oligomeric compound comprises a conjugate group. In certain embodiments, the first modified oligonucleotide is 12-30 linked nucleosides in length and the second oligonucleotide is 12-30 linked nucleosides in length. In certain embodiments, the second oligonucleotide is modified. In certain embodiments, the first modified oligonucleotide has a nucleobase sequence comprising at least 8 contiguous nucleobases of any of SEQ ID NOs: 6-1954. 
     Examples of single-stranded and double-stranded compounds include but are not limited to oligonucleotides, siRNAs, microRNA targeting oligonucleotides, and single-stranded RNAi compounds, such as small hairpin RNAs (shRNAs), single-stranded siRNAs (ssRNAs), and microRNA mimics. 
     In certain embodiments, a compound described herein has a nucleobase sequence that, when written in the 5′ to 3′ direction, comprises the reverse complement of the target segment of a target nucleic acid to which it is targeted. 
     In certain embodiments, a compound described herein comprises an oligonucleotide 10 to 30 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 12 to 30 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 12 to 22 linked subunits in length. In certain embodiments, compound described herein comprises an oligonucleotide 14 to 30 linked subunits in length. In certain embodiments, compound described herein comprises an oligonucleotide 14 to 20 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 15 to 30 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 15 to 20 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 16 to 30 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 16 to 20 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 17 to 30 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 17 to 20 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 18 to 30 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 18 to 21 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 18 to 20 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 20 to 30 linked subunits in length. In other words, such oligonucleotides are 12 to 30 linked subunits, 14 to 30 linked subunits, 14 to 20 subunits, 15 to 30 subunits, 15 to 20 subunits, 16 to 30 subunits, 16 to 20 subunits, 17 to 30 subunits, 17 to 20 subunits, 18 to 30 subunits, 18 to 20 subunits, 18 to 21 subunits, 20 to 30 subunits, or 12 to 22 linked subunits in length, respectively. In certain embodiments, a compound described herein comprises an oligonucleotide 14 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 16 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 17 linked subunits in length. In certain embodiments, compound described herein comprises an oligonucleotide 18 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 19 linked subunits in length. In certain embodiments, a compound described herein comprises an oligonucleotide 20 linked subunits in length. In other embodiments, a compound described herein comprises an oligonucleotide 8 to 80, 12 to 50, 13 to 30, 13 to 50, 14 to 30, 14 to 50, 15 to 30, 15 to 50, 16 to 30, 16 to 50, 17 to 30, 17 to 50, 18 to 22, 18 to 24, 18 to 30, 18 to 50, 19 to 22, 19 to 30, 19 to 50, or 20 to 30 linked subunits. In certain such embodiments, the compound described herein comprises an oligonucleotide 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 linked subunits in length, or a range defined by any two of the above values. In some embodiments the linked subunits are nucleotides, nucleosides, or nucleobases. 
     In certain embodiments, the compound may further comprise additional features or elements, such as a conjugate group, that are attached to the oligonucleotide. In certain embodiments, such compounds are antisense compounds. In certain embodiments, such compounds are oligomeric compounds. In embodiments where a conjugate group comprises a nucleoside (i.e. a nucleoside that links the conjugate group to the oligonucleotide), the nucleoside of the conjugate group is not counted in the length of the oligonucleotide. 
     In certain embodiments, compounds may be shortened or truncated. For example, a single subunit may be deleted from the 5′ end (5′ truncation), or alternatively from the 3′ end (3′ truncation). A shortened or truncated compound targeted to an α-ENaC nucleic acid may have two subunits deleted from the 5′ end, or alternatively may have two subunits deleted from the 3′ end, of the compound. Alternatively, the deleted nucleosides may be dispersed throughout the compound. 
     When a single additional subunit is present in a lengthened compound, the additional subunit may be located at the 5′ or 3′ end of the compound. When two or more additional subunits are present, the added subunits may be adjacent to each other, for example, in a compound having two subunits added to the 5′ end (5′ addition), or alternatively to the 3′ end (3′ addition), of the compound. Alternatively, the added subunits may be dispersed throughout the compound. 
     It is possible to increase or decrease the length of a compound, such as an oligonucleotide, and/or introduce mismatch bases without eliminating activity (Woolf et al.  Proc. Natl. Acad. Sci. USA  1992, 89:7305-7309; Gautschi et al.  J. Natl. Cancer Inst . March 2001, 93:463-471; Maher and Dolnick  Nuc. Acid. Res.  1998, 16:3341-3358). However, seemingly small changes in oligonucleotide sequence, chemistry and motif can make large differences in one or more of the many properties required for clinical development (Seth et al.  J. Med. Chem.  2009, 52, 10; Egli et al.  J. Am. Chem. Soc.  2011, 133, 16642). 
     In certain embodiments, compounds described herein are interfering RNA compounds (RNAi), which include double-stranded RNA compounds (also referred to as short-interfering RNA or siRNA) and single-stranded RNAi compounds (or ssRNA). Such compounds work at least in part through the RISC pathway to degrade and/or sequester a target nucleic acid (thus, include microRNA/microRNA-mimic compounds). As used herein, the term siRNA is meant to be equivalent to other terms used to describe nucleic acid molecules that are capable of mediating sequence specific RNAi, for example short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), short hairpin RNA (shRNA), short interfering oligonucleotide, short interfering nucleic acid, short interfering modified oligonucleotide, chemically modified siRNA, post-transcriptional gene silencing RNA (ptgsRNA), and others. In addition, as used herein, the term “RNAi” is meant to be equivalent to other terms used to describe sequence specific RNA interference, such as post transcriptional gene silencing, translational inhibition, or epigenetics. 
     In certain embodiments, a compound described herein can comprise any of the oligonucleotide sequences targeted to an α-ENaC nucleic acid transcript described herein. In certain embodiments, the compound can be double-stranded. In certain embodiments, the compound comprises a first strand comprising at least an 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 contiguous nucleobase portion of any one of SEQ ID NOs: 6-1954 and a second strand. In certain embodiments, the compound comprises a first strand comprising the nucleobase sequence of any one of SEQ ID NOs: 6-1954 and a second strand. In certain embodiments, the compound comprises ribonucleotides in which the first strand has uracil (U) in place of thymine (T) in any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises (i) a first strand comprising a nucleobase sequence complementary to the site on an α-ENaC nucleic acid to which any of SEQ ID NOs: 6-1954 is complementary, and (ii) a second strand. In certain embodiments, the compound comprises one or more modified nucleotides in which the 2′ position in the sugar contains a halogen (such as fluorine group; 2′-F) or contains an alkoxy group (such as a methoxy group; 2′-OMe). In certain embodiments, the compound comprises at least one 2′-F sugar modification and at least one 2′-OMe sugar modification. In certain embodiments, the at least one 2′-F sugar modification and at least one 2′-OMe sugar modification are arranged in an alternating pattern for at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 contiguous nucleobases along a strand of the dsRNA compound. In certain embodiments, the compound comprises one or more linkages between adjacent nucleotides other than a naturally-occurring phosphodiester linkage. Examples of such linkages include phosphoramide, phosphorothioate, and phosphorodithioate linkages. The compounds may also be chemically modified nucleic acid molecules as taught in U.S. Pat. No. 6,673,661. In other embodiments, the compound contains one or two capped strands, as disclosed, for example, by WO 00/63364, filed Apr. 19, 2000. 
     In certain embodiments, the first strand of the compound is an siRNA guide strand and the second strand of the compound is an siRNA passenger strand. In certain embodiments, the second strand of the compound is complementary to the first strand. In certain embodiments, each strand of the compound is 16, 17, 18, 19, 20, 21, 22, or 23 linked nucleosides in length. In certain embodiments, the first or second strand of the compound can comprise a conjugate group. 
     In certain embodiments, a compound described herein can comprise any of the oligonucleotide sequences targeted to an α-ENaC nucleic acid described herein. In certain embodiments, the compound is single-stranded. In certain embodiments, such a compound is a single-stranded RNAi (ssRNAi) compound. In certain embodiments, the compound comprises at least an 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 contiguous nucleobase portion of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises the nucleobase sequence of any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises ribonucleotides in which uracil (U) is in place of thymine (T) in any one of SEQ ID NOs: 6-1954. In certain embodiments, the compound comprises a nucleobase sequence complementary to the site on α-ENaC to which any of SEQ ID NOs: 6-1954 is targeted. In certain embodiments, the compound comprises one or more modified nucleotides in which the 2′ position in the sugar contains a halogen (such as fluorine group; 2′-F) or contains an alkoxy group (such as a methoxy group; 2′-OMe). In certain embodiments, the compound comprises at least one 2′-F sugar modification and at least one 2′-OMe sugar modification. In certain embodiments, the at least one 2′-F sugar modification and at least one 2′-OMe sugar modification are arranged in an alternating pattern for at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 contiguous nucleobases along a strand of the compound. In certain embodiments, the compound comprises one or more linkages between adjacent nucleotides other than a naturally-occurring phosphodiester linkage. Examples of such linkages include phosphoramide, phosphorothioate, and phosphorodithioate linkages. The compounds may also be chemically modified nucleic acid molecules as taught in U.S. Pat. No. 6,673,661. In other embodiments, the compound contains a capped strand, as disclosed, for example, by WO 00/63364, filed Apr. 19, 2000. In certain embodiments, the compound consists of 16, 17, 18, 19, 20, 21, 22, or 23 linked nucleosides. In certain embodiments, the compound can comprise a conjugate group. 
     Certain compounds described herein (e.g., modified oligonucleotides) have one or more asymmetric center and thus give rise to enantiomers, diastereomers, and other stereoisomeric configurations that may be defined, in terms of absolute stereochemistry, as (R) or (S), as α or β, such as for sugar anomers, or as (D) or (L), such as for amino acids, etc. Compounds provided herein that are drawn or described as having certain stereoisomeric configurations include only the indicated compounds. Compounds provided herein that are drawn or described with undefined stereochemistry include all such possible isomers, including their stereorandom and optically pure forms. All tautomeric forms of the compounds provided herein are included unless otherwise indicated. 
     The compounds described herein include variations in which one or more atoms are replaced with a non-radioactive isotope or radioactive isotope of the indicated element. For example, compounds herein that comprise hydrogen atoms encompass all possible deuterium substitutions for each of the  1 H hydrogen atoms. Isotopic substitutions encompassed by the compounds herein include but are not limited to:  2 H or  3 H in place of  1 H,  13 C or  14 C in place of  12 C,  15 N in place of  14 N,  17 O or  18 O in place of  16 O, and  33 S,  34 S,  35 S, or  36 S in place of  32 S. In certain embodiments, non-radioactive isotopic substitutions may impart new properties on the oligomeric compound that are beneficial for use as a therapeutic or research tool. In certain embodiments, radioactive isotopic substitutions may make the compound suitable for research or diagnostic purposes such as imaging. 
     Certain Mechanisms 
     In certain embodiments, compounds described herein comprise or consist of modified oligonucleotides. In certain embodiments, compounds described herein are antisense compounds. In certain embodiments, compounds comprise oligomeric compounds. In certain embodiments, compounds described herein are capable of hybridizing to an α-ENaC target nucleic acid, resulting in at least one antisense activity. In certain embodiments, compounds described herein selectively affect one or more target nucleic acid. Such compounds comprise a nucleobase sequence that hybridizes to one or more target nucleic acid, resulting in one or more desired antisense activity and does not hybridize to one or more non-target nucleic acid or does not hybridize to one or more non-target nucleic acid in such a way that results in a significant undesired antisense activity. 
     In certain antisense activities, hybridization of a compound described herein to a target nucleic acid results in recruitment of a protein that cleaves the target nucleic acid. For example, certain compounds described herein result in RNase H mediated cleavage of the target nucleic acid. RNase H is a cellular endonuclease that cleaves the RNA strand of an RNA:DNA duplex. The DNA in such an RNA:DNA duplex need not be unmodified DNA. In certain embodiments, compounds described herein are sufficiently “DNA-like” to elicit RNase H activity. Further, in certain embodiments, one or more non-DNA-like nucleoside in the gap of a gapmer is tolerated. 
     In certain antisense activities, compounds described herein or a portion of the compound is loaded into an RNA-induced silencing complex (RISC), ultimately resulting in cleavage of the target nucleic acid. For example, certain compounds described herein result in cleavage of the target nucleic acid by Argonaute. Compounds that are loaded into RISC are RNAi compounds. RNAi compounds may be double-stranded (siRNA) or single-stranded (ssRNA). 
     Antisense activities may be observed directly or indirectly. In certain embodiments, observation or detection of an antisense activity involves observation or detection of a change in an amount of a target nucleic acid or protein encoded by such target nucleic acid, a change in the ratio of splice variants of a nucleic acid or protein, and/or a phenotypic change in a cell or animal. 
     Target Nucleic Acids, Target Regions and Nucleotide Sequences 
     In certain embodiments, compounds described herein comprise or consist of an oligonucleotide comprising a region that is complementary to a target nucleic acid. In certain embodiments, the target nucleic acid is an endogenous RNA molecule. In certain embodiments, the target nucleic acid encodes a protein. In certain such embodiments, the target nucleic acid is selected from: an mRNA and a pre-mRNA, including intronic, exonic and untranslated regions. In certain embodiments, the target RNA is an mRNA. In certain embodiments, the target nucleic acid is a pre-mRNA. In certain embodiments, a pre-mRNA and corresponding mRNA are both target nucleic acids of a single compound. In certain such embodiments, the target region is entirely within an intron of a target pre-mRNA. In certain embodiments, the target region spans an intron/exon junction. In certain embodiments, the target region is at least 50% within an intron. Target nucleic acid sequences that encode α-ENaC include, without limitation, the following: Ref SEQ No. NM_001038.5; the complement of NC_000012.12 truncated from nucleosides 6343001 to 6380000; and NG_011945.1 (SEQ ID Nos: 1, 2, and 1957, respectively).
     Hybridization   

     In some embodiments, hybridization occurs between a compound disclosed herein and an α-ENaC nucleic acid. The most common mechanism of hybridization involves hydrogen bonding (e.g., Watson-Crick, Hoogsteen or reversed Hoogsteen hydrogen bonding) between complementary nucleobases of the nucleic acid molecules. 
     Hybridization can occur under varying conditions. Hybridization conditions are sequence-dependent and are determined by the nature and composition of the nucleic acid molecules to be hybridized. 
     Methods of determining whether a sequence is specifically hybridizable to a target nucleic acid are well known in the art. In certain embodiments, the compounds provided herein are specifically hybridizable with an α-ENaC nucleic acid. 
     Complementarity 
     In certain embodiments, compounds described herein comprise or consist of modified oligonucleotides. In certain embodiments, compounds described herein are antisense compounds. In certain embodiments, compounds comprise oligomeric compounds. In certain embodiments, oligonucleotides complementary to an α-ENaC nucleic acid comprise nucleobase that are non-complementary with the α-ENaC nucleic acid, yet may be tolerated provided that the compound remains able to specifically hybridize to a target nucleic acid. Moreover, a compound may hybridize over one or more segments of an α-ENaC nucleic acid such that intervening or adjacent segments are not involved in the hybridization event (e.g., a loop structure, mismatch or hairpin structure). 
     In certain embodiments, the compounds provided herein, or a specified portion thereof, are, are at least, or are up to 70%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% complementary to an α-ENaC nucleic acid, a target region, target segment, or specified portion thereof. In certain embodiments, the compounds provided herein, or a specified portion thereof, are 70% to 75%, 75% to 80%, 80% to 85%, 85% to 90%, 90% to 95%, 95% to 100%, or any number in between these ranges, complementary to an α-ENaC nucleic acid, a target region, target segment, or specified portion thereof. Percent complementarity of a compound with a target nucleic acid can be determined using routine methods. 
     For example, a compound in which 18 of 20 nucleobases of the compound are complementary to a target region, and would therefore specifically hybridize, would represent 90 percent complementarity. In this example, the remaining non-complementary nucleobases may be clustered or interspersed with complementary nucleobases and need not be contiguous to each other or to complementary nucleobases. As such, a compound which is 18 nucleobases in length having four non-complementary nucleobases which are flanked by two regions of complete complementarity with the target nucleic acid would have 77.8% overall complementarity with the target nucleic acid. Percent complementarity of a compound with a region of a target nucleic acid can be determined routinely using BLAST programs (basic local alignment search tools) and PowerBLAST programs known in the art (Altschul et al.,  J. Mol. Biol.,  1990, 215, 403 410; Zhang and Madden, Genome Res., 1997, 7, 649 656). Percent homology, sequence identity or complementarity, can be determined by, for example, the Gap program (Wisconsin Sequence Analysis Package, Version 8 for Unix, Genetics Computer Group, University Research Park, Madison Wis.), using default settings, which uses the algorithm of Smith and Waterman (Adv. Appl. Math., 1981, 2, 482 489). 
     In certain embodiments, compounds described herein, or specified portions thereof, are fully complementary (i.e. 100% complementary) to a target nucleic acid, or specified portion thereof. For example, a compound may be 100% complementary to an α-ENaC nucleic acid, or a target region, or a target segment or target sequence thereof. As used herein, “fully complementary” means each nucleobase of a compound is complementary to the corresponding nucleobase of a target nucleic acid. For example, a 20 nucleobase compound is fully complementary to a target sequence that is 400 nucleobases long, so long as there is a corresponding 20 nucleobase portion of the target nucleic acid that is fully complementary to the compound. Fully complementary can also be used in reference to a specified portion of the first and/or the second nucleic acid. For example, a 20 nucleobase portion of a 30 nucleobase compound can be “fully complementary” to a target sequence that is 400 nucleobases long. The 20 nucleobase portion of the 30 nucleobase compound is fully complementary to the target sequence if the target sequence has a corresponding 20 nucleobase portion wherein each nucleobase is complementary to the 20 nucleobase portion of the compound. At the same time, the entire 30 nucleobase compound may or may not be fully complementary to the target sequence, depending on whether the remaining 10 nucleobases of the compound are also complementary to the target sequence. 
     In certain embodiments, compounds described herein comprise one or more mismatched nucleobases relative to the target nucleic acid. In certain such embodiments, antisense activity against the target is reduced by such mismatch, but activity against a non-target is reduced by a greater amount. Thus, in certain such embodiments selectivity of the compound is improved. In certain embodiments, the mismatch is specifically positioned within an oligonucleotide having a gapmer motif. In certain such embodiments, the mismatch is at position 1, 2, 3, 4, 5, 6, 7, or 8 from the 5′-end of the gap segment. In certain such embodiments, the mismatch is at position 9, 8, 7, 6, 5, 4, 3, 2, 1 from the 3′-end of the gap segment. In certain such embodiments, the mismatch is at position 1, 2, 3, or 4 from the 5′-end of the wing segment. In certain such embodiments, the mismatch is at position 4, 3, 2, or 1 from the 3′-end of the wing segment. In certain embodiments, the mismatch is specifically positioned within an oligonucleotide not having a gapmer motif. In certain such embodiments, the mismatch is at position 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 from the 5′-end of the oligonucleotide. In certain such embodiments, the mismatch is at position, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 from the 3′-end of the oligonucleotide. 
     The location of a non-complementary nucleobase may be at the 5′ end or 3′ end of the compound. Alternatively, the non-complementary nucleobase or nucleobases may be at an internal position of the compound. When two or more non-complementary nucleobases are present, they may be contiguous (i.e. linked) or non-contiguous. In one embodiment, a non-complementary nucleobase is located in the wing segment of a gapmer oligonucleotide. 
     In certain embodiments, compounds described herein that are, or are up to 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleobases in length comprise no more than 4, no more than 3, no more than 2, or no more than 1 non-complementary nucleobase(s) relative to a target nucleic acid, such as an α-ENaC nucleic acid, or specified portion thereof. 
     In certain embodiments, compounds described herein that are, or are up to 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 nucleobases in length comprise no more than 6, no more than 5, no more than 4, no more than 3, no more than 2, or no more than 1 non-complementary nucleobase(s) relative to a target nucleic acid, such as an α-ENaC nucleic acid, or specified portion thereof. 
     In certain embodiments, compounds described herein also include those which are complementary to a portion (a defined number of contiguous nucleobases within a region or segment) of a target nucleic acid. In certain embodiments, the compounds, are complementary to at least an 8 nucleobase portion of a target segment. In certain embodiments, the compounds are complementary to at least a 9 nucleobase portion of a target segment. In certain embodiments, the compounds are complementary to at least a 10 nucleobase portion of a target segment. In certain embodiments, the compounds are complementary to at least an 11 nucleobase portion of a target segment. In certain embodiments, the compounds are complementary to at least a 12 nucleobase portion of a target segment. In certain embodiments, the compounds are complementary to at least a 13 nucleobase portion of a target segment. In certain embodiments, the compounds are complementary to at least a 14 nucleobase portion of a target segment. In certain embodiments, the compounds are complementary to at least a 15 nucleobase portion of a target segment. In certain embodiments, the compounds are complementary to at least a 16 nucleobase portion of a target segment. Also contemplated are compounds that are complementary to at least a 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or more nucleobase portion of a target segment, or a range defined by any two of these values. 
     Certain Compounds 
     In certain embodiments, compounds described herein comprise or consist of oligonucleotides consisting of linked nucleosides. Oligonucleotides may be unmodified oligonucleotides (RNA or DNA) or may be modified oligonucleotides. Modified oligonucleotides comprise at least one modification relative to unmodified RNA or DNA (i.e., comprise at least one modified nucleoside (comprising a modified sugar moiety and/or a modified nucleobase) and/or at least one modified internucleoside linkage). 
     I. Modifications 
     A. Modified Nucleosides 
     Modified nucleosides comprise a modified sugar moiety or a modified nucleobase or both a modified sugar moiety and a modified nucleobase. 
     1. Modified Sugar Moieties 
     In certain embodiments, sugar moieties are non-bicyclic modified sugar moieties. In certain embodiments, modified sugar moieties are bicyclic or tricyclic sugar moieties. In certain embodiments, modified sugar moieties are sugar surrogates. Such sugar surrogates may comprise one or more substitutions corresponding to those of other types of modified sugar moieties. 
     In certain embodiments, modified sugar moieties are non-bicyclic modified furanosyl sugar moieties comprising one or more acyclic substituent, including but not limited to substituents at the 2′, 4′, and/or 5′ positions. In certain embodiments, the furanosyl sugar moiety is a ribosyl sugar moiety. In certain embodiments one or more acyclic substituent of non-bicyclic modified sugar moieties is branched. Examples of 2′-substituent groups suitable for non-bicyclic modified sugar moieties include but are not limited to: 2′-F, 2′-OCH 3 (“OMe” or “O-methyl”), and 2′-O(CH 2 ) 2 OCH 3  (“MOE”). In certain embodiments, 2′-substituent groups are selected from among: halo, allyl, amino, azido, SH, CN, OCN, CF 3 , OCF 3 , O—C 1 -C 10  alkoxy, O—C 1 -C 10  substituted alkoxy, O—C 1 -C 10  alkyl, O—C 1 -C 10  substituted alkyl, S-alkyl, N(R m )-alkyl, O-alkenyl, S-alkenyl, N(R m )-alkenyl, O-alkynyl, S-alkynyl, N(R m )-alkynyl, O-alkylenyl-O-alkyl, alkynyl, alkaryl, aralkyl, O-alkaryl, O-aralkyl, O(CH 2 ) 2 SCH 3 , O(CH 2 ) 2 ON(R m )(R n ) or OCH 2 C(═O)—N(R m )(R n ), where each R m  and R n  is, independently, H, an amino protecting group, or substituted or unsubstituted C 1 -C 10  alkyl, and the 2′-substituent groups described in Cook et al., U.S. Pat. No. 6,531,584; Cook et al., U.S. Pat. No. 5,859,221; and Cook et al., U.S. Pat. No. 6,005,087. Certain embodiments of these 2′-substituent groups can be further substituted with one or more substituent groups independently selected from among: hydroxyl, amino, alkoxy, carboxy, benzyl, phenyl, nitro (NO 2 ), thiol, thioalkoxy, thioalkyl, halogen, alkyl, aryl, alkenyl and alkynyl. Examples of 4′-substituent groups suitable for non-bicyclic modified sugar moieties include but are not limited to alkoxy (e.g., methoxy), alkyl, and those described in Manoharan et al., WO 2015/106128. Examples of 5′-substituent groups suitable for non-bicyclic modified sugar moieties include but are not limited to: 5′-methyl (R or S), 5′-vinyl, and 5′-methoxy. In certain embodiments, non-bicyclic modified sugars comprise more than one non-bridging sugar substituent, for example, 2′-F-5′-methyl sugar moieties and the modified sugar moieties and modified nucleosides described in Migawa et al., WO 2008/101157 and Rajeev et al., US2013/0203836.). 
     In certain embodiments, a 2′-substituted nucleoside or 2′-non-bicyclic modified nucleoside comprises a sugar moiety comprising a non-bridging 2′-substituent group selected from: F, NH 2 , N 3 , OCF 3 , OCH 3 , O(CH 2 ) 3 NH 2 , CH 2 CH═CH 2 , OCH 2 CH═CH 2 , OCH 2 CH 2 OCH 3 , O(CH 2 ) 2 SCH 3 , O(CH 2 ) 2 ON(R m )(R n ), O(CH 2 ) 2 O(CH 2 ) 2 N(CH 3 ) 2 , and N-substituted acetamide (OCH 2 C(═O)—N(R m )(R n )), where each R m  and R n  is, independently, H, an amino protecting group, or substituted or unsubstituted C 1 -C 10  alkyl. 
     In certain embodiments, a 2′-substituted nucleoside or 2′-non-bicyclic modified nucleoside comprises a sugar moiety comprising a non-bridging 2′-substituent group selected from: F, OCF 3 , OCH 3 , OCH 2 CH 2 OCH 3 , O(CH 2 ) 2 SCH 3 , O(CH 2 ) 2 ON(CH 3 ) 2 , O(CH 2 ) 2 O(CH 2 ) 2 N(CH 3 ) 2 , and OCH 2 C(═O)—N(H)CH 3  (“NMA”). 
     In certain embodiments, a 2′-substituted nucleoside or 2′-non-bicyclic modified nucleoside comprises a sugar moiety comprising a non-bridging 2′-substituent group selected from: F, OCH 3 , and OCH 2 CH 2 OCH 3 . 
     Nucleosides comprising modified sugar moieties, such as non-bicyclic modified sugar moieties, may be referred to by the position(s) of the substitution(s) on the sugar moiety of the nucleoside. For example, nucleosides comprising 2′-substituted or 2-modified sugar moieties are referred to as 2′-substituted nucleosides or 2-modified nucleosides. 
     Certain modified sugar moieties comprise a bridging sugar substituent that forms a second ring resulting in a bicyclic sugar moiety. In certain such embodiments, the bicyclic sugar moiety comprises a bridge between the 4′ and the 2′ furanose ring atoms. In certain such embodiments, the furanose ring is a ribose ring. Examples of such 4′ to 2′ bridging sugar substituents include but are not limited to: 4′-CH 2 -2′, 4′-(CH 2 ) 2 -2′, 4′-(CH 2 ) 3 -2′, 4′-CH 2 —O-2′ (“LNA”), 4′-CH 2 —S-2′, 4′-(CH 2 ) 2 —O-2′ (“ENA”), 4′-CH(CH 3 )—O-2′ (referred to as “constrained ethyl” or “cEt” when in the S configuration), 4′-CH 2 —O—CH 2 -2′, 4′-CH 2 —N(R)-2′, 4′-CH(CH 2 OCH 3 )—O-2′ (“constrained MOE” or “cMOE”) and analogs thereof (see, e.g., Seth et al., U.S. Pat. No. 7,399,845, Bhat et al., U.S. Pat. No. 7,569,686, Swayze et al., U.S. Pat. No. 7,741,457, and Swayze et al., U.S. Pat. No. 8,022,193), 4′-C(CH 3 )(CH 3 )—O-2′ and analogs thereof (see, e.g., Seth et al., U.S. Pat. No. 8,278,283), 4′-CH 2 —N(OCH 3 )-2′ and analogs thereof (see, e.g., Prakash et al., U.S. Pat. No. 8,278,425), 4′-CH 2 —O—N(CH 3 )-2′ (see, e.g., Allerson et al., U.S. Pat. No. 7,696,345 and Allerson et al., U.S. Pat. No. 8,124,745), 4′-CH 2 —C(H)(CH 3 )-2′ (see, e.g., Zhou, et al.,  J. Org. Chem.,  2009, 74, 118-134), 4′-CH 2 —C(═CH 2 )-2′ and analogs thereof (see e.g., Seth et al., U.S. Pat. No. 8,278,426), 4′-C(R a R b )—N(R)—O-2′, 4′-C(R a R b )—O—N(R)-2′, 4′-CH 2 —O—N(R)-2′, and 4′-CH 2 —N(R)—O-2′, wherein each R, R a , and R b  is, independently, H, a protecting group, or C 1 -C 12  alkyl (see, e.g. Imanishi et al., U.S. Pat. No. 7,427,672). 
     In certain embodiments, such 4′ to 2′ bridges independently comprise from 1 to 4 linked groups independently selected from: —[C(R a )(R b )] n —, —[C(R a )(R b )] n —O—, —C(R a )═C(R b )—, —C(R a )═N—, —C(═NR a )—, —C(═O)—, —C(═S)—, —O—, —Si(R a ) 2 —, —S(═O) x —, and —N(R a )—; 
     wherein: 
     x is 0, 1, or 2; 
     n is 1, 2, 3, or 4; 
     each R a  and R b  is, independently, H, a protecting group, hydroxyl, C 1 -C 12  alkyl, substituted C 1 -C 12  alkyl, C 2 -C 12  alkenyl, substituted C 2 -C 12  alkenyl, C 2 -C 12  alkynyl, substituted C 2 -C 12  alkynyl, C 5 -C 20  aryl, substituted C 5 -C 20  aryl, heterocycle radical, substituted heterocycle radical, heteroaryl, substituted heteroaryl, C 5 -C 7  alicyclic radical, substituted C 5 -C 7  alicyclic radical, halogen, OJ 1 , NJ 1 J 2 , SJ 1 , N 3 , COOJ 1 , acyl (C(═O)—H), substituted acyl, CN, sulfonyl (S(═O) 2 -J 1 ), or sulfoxyl (S(═O)-J 1 ); and 
     each J 1  and J 2  is, independently, H, C 1 -C 12  alkyl, substituted C 1 -C 12  alkyl, C 2 -C 12  alkenyl, substituted C 2 -C 12  alkenyl, C 2 -C 12  alkynyl, substituted C 2 -C 12  alkynyl, C 5 -C 20  aryl, substituted C 5 -C 20  aryl, acyl (C(═O)—H), substituted acyl, a heterocycle radical, a substituted heterocycle radical, C 1 -C 12  aminoalkyl, substituted C 1 -C 12  aminoalkyl, or a protecting group. 
     Additional bicyclic sugar moieties are known in the art, see, for example: Freier et al.,  Nucleic Acids Research,  1997, 25(22), 4429-4443, Albaek et al.,  J. Org. Chem.,  2006, 71, 7731-7740, Singh et al.,  Chem. Commun.,  1998, 4, 455-456; Koshkin et al.,  Tetrahedron,  1998, 54, 3607-3630; Kumar et al.,  Bioorg. Med. Chem. Lett.,  1998, 8, 2219-2222; Singh et al.,  J. Org. Chem.,  1998, 63, 10035-10039; Srivastava et al.,  J. Am. Chem. Soc.,  20017, 129, 8362-8379; Elayadi et al., Wengel et a., U.S. Pat. No. 7,053,207; Imanishi et al., U.S. Pat. No. 6,268,490; Imanishi et al. U.S. Pat. No. 6,770,748; Imanishi et al., U.S. RE44,779; Wengel et al., U.S. Pat. No. 6,794,499; Wengel et al., U.S. Pat. No. 6,670,461; Wengel et al., U.S. Pat. No. 7,034,133; Wengel et al., U.S. Pat. No. 8,080,644; Wengel et al., U.S. Pat. No. 8,034,909; Wengel et al., U.S. Pat. No. 8,153,365; Wengel et al., U.S. Pat. No. 7,572,582; and Ramasamy et al., U.S. Pat. No. 6,525,191; Torsten et al., WO 2004/106356; Wengel et al., WO 1999/014226; Seth et al., WO 2007/134181; Seth et al., U.S. Pat. No. 7,547,684; Seth et al., U.S. Pat. No. 7,666,854; Seth et al., U.S. Pat. No. 8,088,746; Seth et al., U.S. Pat. No. 7,750,131; Seth et al., U.S. Pat. No. 8,030,467; Seth et al., U.S. Pat. No. 8,268,980; Seth et al., U.S. Pat. No. 8,546,556; Seth et al., U.S. Pat. No. 8,530,640; Migawa et al., U.S. Pat. No. 9,012,421; Seth et al., U.S. Pat. No. 8,501,805; and U.S. Patent Publication Nos. Allerson et al., US2008/0039618 and Migawa et al., US2015/0191727. 
     In certain embodiments, bicyclic sugar moieties and nucleosides incorporating such bicyclic sugar moieties are further defined by isomeric configuration. For example, an LNA nucleoside (described herein) may be in the α-L configuration or in the β-D configuration. 
     
       
         
         
             
             
         
       
     
     α-L-methyleneoxy (4′-CH 2 —O-2′) or α-L-LNA bicyclic nucleosides have been incorporated into antisense oligonucleotides that showed antisense activity (Frieden et al.,  Nucleic Acids Research,  2003, 21, 6365-6372). Herein, general descriptions of bicyclic nucleosides include both isomeric configurations. When the positions of specific bicyclic nucleosides (e.g., LNA) are identified in exemplified embodiments herein, they are in the β-D configuration, unless otherwise specified. 
     In certain embodiments, modified sugar moieties comprise one or more non-bridging sugar substituent and one or more bridging sugar substituent (e.g., 5′-substituted and 4′-2′ bridged sugars). 
     In certain embodiments, modified sugar moieties are sugar surrogates. In certain such embodiments, the oxygen atom of the sugar moiety is replaced, e.g., with a sulfur, carbon or nitrogen atom. In certain such embodiments, such modified sugar moieties also comprise bridging and/or non-bridging substituents as described herein. For example, certain sugar surrogates comprise a 4′-sulfur atom and a substitution at the 2′-position (see, e.g., Bhat et al., U.S. Pat. No. 7,875,733 and Bhat et al., U.S. Pat. No. 7,939,677) and/or the 5′ position. 
     In certain embodiments, sugar surrogates comprise rings having other than 5 atoms. For example, in certain embodiments, a sugar surrogate comprises a six-membered tetrahydropyran (“THP”). Such tetrahydropyrans may be further modified or substituted. Nucleosides comprising such modified tetrahydropyrans include but are not limited to hexitol nucleic acid (“HNA”), anitol nucleic acid (“ANA”), manitol nucleic acid (“MNA”) (see, e.g., Leumann, C J.  Bioorg . &amp;  Med. Chem.  2002, 10, 841-854), fluoro HNA: 
     
       
         
         
             
             
         
       
     
     (“F-HNA”, see e.g. Swayze et al., U.S. Pat. No. 8,088,904; Swayze et al., U.S. Pat. No. 8,440,803; Swayze et al., U.S. Pat. No. 8,796,437; and Swayze et al., U.S. Pat. No. 9,005,906; F-HNA can also be referred to as a F-THP or 3′-fluoro tetrahydropyran), and nucleosides comprising additional modified THP compounds having the formula: 
     
       
         
         
             
             
         
       
     
     wherein, independently, for each of said modified THP nucleoside: 
     Bx is a nucleobase moiety; 
     T 3  and T 4  are each, independently, an internucleoside linking group linking the modified THP nucleoside to the remainder of an oligonucleotide or one of T 3  and T 4  is an internucleoside linking group linking the modified THP nucleoside to the remainder of an oligonucleotide and the other of T 3  and T 4  is H, a hydroxyl protecting group, a linked conjugate group, or a 5′ or 3′-terminal group; 
     q 1 , q 2 , q 3 , q 4 , q 5 , q 6  and q 7  are each, independently, H, C 1 -C 6  alkyl, substituted C 1 -C 6  alkyl, C 2 -C 6  alkenyl, substituted C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, or substituted C 2 -C 6  alkynyl; and 
     each of R 1  and R 2  is independently selected from among: hydrogen, halogen, substituted or unsubstituted alkoxy, NJ 1 J 2 , SJ 1 , N 3 , OC(═X)J 1 , OC(═X)NJ 1 J 2 , NJ 3 C(═X)NJ 1 J 2 , and CN, wherein X is O, S or NJ 1 , and each J 1 , J 2 , and J 3  is, independently, H or C 1 -C 6  alkyl. 
     In certain embodiments, modified THP nucleosides are provided wherein q 1 , q 2 , q 3 , q 4 , q 5 , q 6  and q 7  are each H. In certain embodiments, at least one of q 1 , q 2 , q 3 , q 4 , q 5 , q 6  and q 7  is other than H. In certain embodiments, at least one of q 1 , q 2 , q 3 , q 4 , q 5 , q 6  and q 7  is methyl. In certain embodiments, modified THP nucleosides are provided wherein one of R 1  and R 2  is F. In certain embodiments, R 1  is F and R 2  is H, in certain embodiments, R 1  is methoxy and R 2  is H, and in certain embodiments, R 1  is methoxyethoxy and R 2  is H. 
     In certain embodiments, sugar surrogates comprise rings having more than 5 atoms and more than one heteroatom. For example, nucleosides comprising morpholino sugar moieties and their use in oligonucleotides have been reported (see, e.g., Braasch et al., Biochemistry, 2002, 41, 4503-4510 and Summerton et al., U.S. Pat. No. 5,698,685; Summerton et al., U.S. Pat. No. 5,166,315; Summerton et al., U.S. Pat. No. 5,185,444; and Summerton et al., U.S. Pat. No. 5,034,506). As used here, the term “morpholino” means a sugar surrogate having the following structure: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, morpholinos may be modified, for example by adding or altering various substituent groups from the above morpholino structure. Such sugar surrogates are referred to herein as “modified morpholinos.” 
     In certain embodiments, sugar surrogates comprise acyclic moieties. Examples of nucleosides and oligonucleotides comprising such acyclic sugar surrogates include but are not limited to: peptide nucleic acid (“PNA”), acyclic butyl nucleic acid (see, e.g., Kumar et al.,  Org. Biomol. Chem.,  2013, 11, 5853-5865), and nucleosides and oligonucleotides described in Manoharan et al., WO2011/133876. 
     Many other bicyclic and tricyclic sugar and sugar surrogate ring systems are known in the art that can be used in modified nucleosides). 
     2. Modified Nucleobases 
     In certain embodiments, modified nucleobases are selected from: 5-substituted pyrimidines, 6-azapyrimidines, alkyl or alkynyl substituted pyrimidines, alkyl substituted purines, and N-2, N-6 and 0-6 substituted purines. In certain embodiments, modified nucleobases are selected from: 2-aminopropyladenine, 5-hydroxymethyl cytosine, xanthine, hypoxanthine, 2-aminoadenine, 6-N-methylguanine, 6-N-methyladenine, 2-propyladenine, 2-thiouracil, 2-thiothymine and 2-thiocytosine, 5-propynyl (—C≡C—CH 3 ) uracil, 5-propynylcytosine, 6-azouracil, 6-azocytosine, 6-azothymine, 5-ribosyluracil (pseudouracil), 4-thiouracil, 8-halo, 8-amino, 8-thiol, 8-thioalkyl, 8-hydroxyl, 8-aza and other 8-substituted purines, 5-halo, particularly 5-bromo, 5-trifluoromethyl, 5-halouracil, and 5-halocytosine, 7-methylguanine, 7-methyladenine, 2-F-adenine, 2-aminoadenine, 7-deazaguanine, 7-deazaadenine, 3-deazaguanine, 3-deazaadenine, 6-N-benzoyladenine, 2-N-isobutyrylguanine, 4-N-benzoylcytosine, 4-N-benzoyluracil, 5-methyl 4-N-benzoylcytosine, 5-methyl 4-N-benzoyluracil, universal bases, hydrophobic bases, promiscuous bases, size-expanded bases, and fluorinated bases. Further modified nucleobases include tricyclic pyrimidines, such as 1,3-diazaphenoxazine-2-one, 1,3-diazaphenothiazine-2-one and 9-(2-aminoethoxy)-1,3-diazaphenoxazine-2-one (G-clamp). Modified nucleobases may also include those in which the purine or pyrimidine base is replaced with other heterocycles, for example 7-deaza-adenine, 7-deazaguanosine, 2-aminopyridine and 2-pyridone. Further nucleobases include those disclosed in Merigan et al., U.S. Pat. No. 3,687,808, those disclosed in  The Concise Encyclopedia Of Polymer Science And Engineering , Kroschwitz, J.I., Ed., John Wiley &amp; Sons, 1990, 858-859; Englisch et al.,  Angewandte Chemie , International Edition, 1991, 30, 613; Sanghvi, Y. S., Chapter 15,  Antisense Research and Applications , Crooke, S. T. and Lebleu, B., Eds., CRC Press, 1993, 273-288; and those disclosed in Chapters 6 and 15,  Antisense Drug Technology , Crooke S.T., Ed., CRC Press, 2008, 163-166 and 442-443. 
     Publications that teach the preparation of certain of the above noted modified nucleobases as well as other modified nucleobases include without limitation, Manohara et al., US2003/0158403; Manoharan et al., US2003/0175906; Dinh et al., U.S. Pat. No. 4,845,205; Spielvogel et al., U.S. Pat. No. 5,130,302; Rogers et al., U.S. Pat. No. 5,134,066; Bischofberger et al., U.S. Pat. No. 5,175,273; Urdea et al., U.S. Pat. No. 5,367,066; Benner et al., U.S. Pat. No. 5,432,272; Matteucci et al., U.S. Pat. No. 5,434,257; Gmeiner et al., U.S. Pat. No. 5,457,187; Cook et al., U.S. Pat. No. 5,459,255; Froehler et al., U.S. Pat. No. 5,484,908; Matteucci et al., U.S. Pat. No. 5,502,177; Hawkins et al., U.S. Pat. No. 5,525,711; Haralambidis et al., U.S. Pat. No. 5,552,540; Cook et al., U.S. Pat. No. 5,587,469; Froehler et al., U.S. Pat. No. 5,594,121; Switzer et al., U.S. Pat. No. 5,596,091; Cook et al., U.S. Pat. No. 5,614,617; Froehler et al., U.S. Pat. No. 5,645,985; Cook et al., U.S. Pat. No. 5,681,941; Cook et al., U.S. Pat. No. 5,811,534; Cook et al., U.S. Pat. No. 5,750,692; Cook et al., U.S. Pat. No. 5,948,903; Cook et al., U.S. Pat. No. 5,587,470; Cook et al., U.S. Pat. No. 5,457,191; Matteucci et al., U.S. Pat. No. 5,763,588; Froehler et al., U.S. Pat. No. 5,830,653; Cook et al., U.S. Pat. No. 5,808,027; Cook et al., 6,166,199; and Matteucci et al., U.S. Pat. No. 6,005,096. 
     In certain embodiments, compounds comprise or consist of a modified oligonucleotide complementary to an α-ENaC nucleic acid comprising one or more modified nucleobases. In certain embodiments, the modified nucleobase is 5-methylcytosine. In certain embodiments, each cytosine is a 5-methylcytosine. 
     B. Modified Internucleoside Linkages 
     In certain embodiments, compounds described herein having one or more modified internucleoside linkages are selected over compounds having only phosphodiester internucleoside linkages because of desirable properties such as, for example, enhanced cellular uptake, enhanced affinity for target nucleic acids, and increased stability in the presence of nucleases. 
     In certain embodiments, compounds comprise or consist of a modified oligonucleotide complementary to an α-ENaC nucleic acid comprising one or more modified internucleoside linkages. In certain embodiments, the modified internucleoside linkages are phosphorothioate linkages. In certain embodiments, each internucleoside linkage of an antisense compound is a phosphorothioate internucleoside linkage. 
     In certain embodiments, nucleosides of modified oligonucleotides may be linked together using any internucleoside linkage. The two main classes of internucleoside linking groups are defined by the presence or absence of a phosphorus atom. Representative phosphorus-containing internucleoside linkages include but are not limited to phosphates, which contain a phosphodiester bond (“P═O”) (also referred to as unmodified or naturally occurring linkages), phosphotriesters, methylphosphonates, phosphoramidates, and phosphorothioates (“P═S”), and phosphorodithioates (“HS—P═S”). Representative non-phosphorus containing internucleoside linking groups include but are not limited to methylenemethylimino (—CH 2 —N(CH 3 )—O—CH 2 —), thiodiester, thionocarbamate (—O—C(═O)(NH)—S—); siloxane (—O—SiH 2 —O—); and N,N′-dimethylhydrazine (—CH 2 —N(CH 3 )—N(CH 3 )—). Modified internucleoside linkages, compared to naturally occurring phosphate linkages, can be used to alter, typically increase, nuclease resistance of the oligonucleotide. Methods of preparation of phosphorous-containing and non-phosphorous-containing internucleoside linkages are well known to those skilled in the art. 
     Representative internucleoside linkages having a chiral center include but are not limited to alkylphosphonates and phosphorothioates. Modified oligonucleotides comprising internucleoside linkages having a chiral center can be prepared as populations of modified oligonucleotides comprising stereorandom internucleoside linkages, or as populations of modified oligonucleotides comprising phosphorothioate linkages in particular stereochemical configurations. In certain embodiments, populations of modified oligonucleotides comprise phosphorothioate internucleoside linkages wherein all of the phosphorothioate internucleoside linkages are stereorandom. Such modified oligonucleotides can be generated using synthetic methods that result in random selection of the stereochemical configuration of each phosphorothioate linkage. Nonetheless, as is well understood by those of skill in the art, each individual phosphorothioate of each individual oligonucleotide molecule has a defined stereoconfiguration. In certain embodiments, populations of modified oligonucleotides are enriched for modified oligonucleotides comprising one or more particular phosphorothioate internucleoside linkages in a particular, independently selected stereochemical configuration. In certain embodiments, the particular configuration of the particular phosphorothioate linkage is present in at least 65% of the molecules in the population. In certain embodiments, the particular configuration of the particular phosphorothioate linkage is present in at least 70% of the molecules in the population. In certain embodiments, the particular configuration of the particular phosphorothioate linkage is present in at least 80% of the molecules in the population. In certain embodiments, the particular configuration of the particular phosphorothioate linkage is present in at least 90% of the molecules in the population. In certain embodiments, the particular configuration of the particular phosphorothioate linkage is present in at least 99% of the molecules in the population. Such chirally enriched populations of modified oligonucleotides can be generated using synthetic methods known in the art, e.g., methods described in Oka et al.,  JACS  125, 8307 (2003), Wan et al.  Nuc. Acid. Res.  42, 13456 (2014), and WO 2017/015555. In certain embodiments, a population of modified oligonucleotides is enriched for modified oligonucleotides having at least one indicated phosphorothioate in the (Sp) configuration. In certain embodiments, a population of modified oligonucleotides is enriched for modified oligonucleotides having at least one phosphorothioate in the (Rp) configuration. In certain embodiments, modified oligonucleotides comprising (Rp) and/or (Sp) phosphorothioates comprise one or more of the following formulas, respectively, wherein “B” indicates a nucleobase: 
     
       
         
         
             
             
         
       
     
     Unless otherwise indicated, chiral internucleoside linkages of modified oligonucleotides described herein can be stereorandom or in a particular stereochemical configuration. 
     Neutral internucleoside linkages include, without limitation, phosphotriesters, methylphosphonates, MMI (3′-CH 2 —N(CH 3 )—O-5′), amide-3 (3′-CH 2 —C(═O)—N(H)-5′), amide-4 (3′-CH 2 —N(H)—C(═O)-5′), formacetal (3′-O—CH 2 —O-5′), methoxypropyl, and thioformacetal (3′-S—CH 2 —O-5′). Further neutral internucleoside linkages include nonionic linkages comprising siloxane (dialkylsiloxane), carboxylate ester, carboxamide, sulfide, sulfonate ester and amides (See for example: Carbohydrate Modifications in Antisense Research; Y. S. Sanghvi and P. D. Cook, Eds., ACS Symposium Series 580; Chapters 3 and 4, 40-65). Further neutral internucleoside linkages include nonionic linkages comprising mixed N, O, S and CH2 component parts. 
     II. Certain Motifs 
     In certain embodiments, compounds described herein comprise or consist of oligonucleotides. Oligonucleotides can have a motif, e.g. a pattern of unmodified and/or modified sugar moieties, nucleobases, and/or internucleoside linkages. In certain embodiments, modified oligonucleotides comprise one or more modified nucleoside comprising a modified sugar. In certain embodiments, modified oligonucleotides comprise one or more modified nucleosides comprising a modified nucleobase. In certain embodiments, modified oligonucleotides comprise one or more modified internucleoside linkage. In such embodiments, the modified, unmodified, and differently modified sugar moieties, nucleobases, and/or internucleoside linkages of a modified oligonucleotide define a pattern or motif. In certain embodiments, the patterns or motifs of sugar moieties, nucleobases, and internucleoside linkages are each independent of one another. Thus, a modified oligonucleotide may be described by its sugar motif, nucleobase motif and/or internucleoside linkage motif (as used herein, nucleobase motif describes the modifications to the nucleobases independent of the sequence of nucleobases). 
     A. Certain Sugar Motifs 
     In certain embodiments, compounds described herein comprise or consist of oligonucleotides. In certain embodiments, oligonucleotides comprise one or more type of modified sugar and/or unmodified sugar moiety arranged along the oligonucleotide or region thereof in a defined pattern or sugar motif. In certain instances, such sugar motifs include but are not limited to any of the sugar modifications discussed herein. 
     In certain embodiments, modified oligonucleotides comprise or consist of a region having a gapmer motif, which comprises two external segments or “wings” and a central or internal segment or “gap.” The three segments of a gapmer motif (the 5′-wing, the gap, and the 3′-wing) form a contiguous sequence of nucleosides wherein at least some of the sugar moieties of the nucleosides of each of the wings differ from at least some of the sugar moieties of the nucleosides of the gap. Specifically, at least the sugar moieties of the nucleosides of each wing that are immediately adjacent to the gap (the 3′-terminal wing nucleoside of the 5′-wing and the 5′-terminal wing nucleoside of the 3′-wing) differ from the sugar moiety of the adjacent gap nucleosides. In certain embodiments, the sugar moieties within the gap are the same as one another. In certain embodiments, the gap includes one or more nucleoside having a sugar moiety that differs from the sugar moiety of one or more other nucleosides of the gap. In certain embodiments, the sugar motifs of the two wings are the same as one another (symmetric gapmer). In certain embodiments, the sugar motif of the 5′-wing differs from the sugar motif of the 3′-wing (asymmetric gapmer). 
     In certain embodiments, the wings of a gapmer each comprise 1-5 nucleosides. In certain embodiments, the wings of a gapmer each comprise 2-5 nucleosides. In certain embodiments, the wings of a gapmer each comprise 3-5 nucleosides. In certain embodiments, the nucleosides of the wings of a gapmer are all modified nucleosides. In certain such embodiments, the sugar moieties of the wings of a gapmer are all modified sugar moieties. 
     In certain embodiments, the gap of a gapmer comprises 7-12 nucleosides. In certain embodiments, the gap of a gapmer comprises 7-10 nucleosides. In certain embodiments, the gap of a gapmer comprises 8-10 nucleosides. In certain embodiments, the gap of a gapmer comprises 10 nucleosides. In certain embodiment, each nucleoside of the gap of a gapmer is a 2′-deoxynucleoside. 
     In certain embodiments, the gapmer is a deoxy gapmer. In such embodiments, the nucleosides on the gap side of each wing/gap junction are 2′-deoxynucleosides and the terminal wing nucleosides immediately adjacent to the gap comprise modified sugar moieties. In certain such embodiments, each nucleoside of the gap is a 2′-deoxynucleoside. In certain such embodiments, each nucleoside of each wing comprises a modified sugar moiety. 
     In certain embodiments, a modified oligonucleotide has a fully modified sugar motif wherein each nucleoside of the modified oligonucleotide comprises a modified sugar moiety. In certain embodiments, modified oligonucleotides comprise or consist of a region having a fully modified sugar motif wherein each nucleoside of the region comprises a modified sugar moiety. In certain embodiments, modified oligonucleotides comprise or consist of a region having a fully modified sugar motif, wherein each nucleoside within the fully modified region comprises the same modified sugar moiety, referred to herein as a uniformly modified sugar motif. In certain embodiments, a fully modified oligonucleotide is a uniformly modified oligonucleotide. In certain embodiments, each nucleoside of a uniformly modified oligonucleotide comprises the same 2′-modification. 
     In certain embodiments, a modified oligonucleotide can comprise a sugar motif described in Swayze et al., US2010/0197762; Freier et al., US2014/0107330; Freier et al., US2015/0184153; and Seth et al., US2015/0267195. 
     B. Certain Nucleobase Motifs 
     In certain embodiments, compounds described herein comprise or consist of oligonucleotides. In certain embodiments, oligonucleotides comprise modified and/or unmodified nucleobases arranged along the oligonucleotide or region thereof in a defined pattern or motif. In certain embodiments, each nucleobase is modified. In certain embodiments, none of the nucleobases are modified. In certain embodiments, each purine or each pyrimidine is modified. In certain embodiments, each adenine is modified. In certain embodiments, each guanine is modified. In certain embodiments, each thymine is modified. In certain embodiments, each uracil is modified. In certain embodiments, each cytosine is modified. In certain embodiments, some or all of the cytosine nucleobases in a modified oligonucleotide are 5-methylcytosines. 
     In certain embodiments, modified oligonucleotides comprise a block of modified nucleobases. In certain such embodiments, the block is at the 3′-end of the oligonucleotide. In certain embodiments the block is within 3 nucleosides of the 3′-end of the oligonucleotide. In certain embodiments, the block is at the 5′-end of the oligonucleotide. In certain embodiments the block is within 3 nucleosides of the 5′-end of the oligonucleotide. 
     In certain embodiments, oligonucleotides having a gapmer motif comprise a nucleoside comprising a modified nucleobase. In certain such embodiments, one nucleoside comprising a modified nucleobase is in the gap of an oligonucleotide having a gapmer motif. In certain such embodiments, the sugar moiety of said nucleoside is a 2′-deoxyribosyl moiety. In certain embodiments, the modified nucleobase is selected from: a 2-thiopyrimidine and a 5-propynepyrimidine. 
     C. Certain Internucleoside Linkage Motifs 
     In certain embodiments, compounds described herein comprise or consist of oligonucleotides. In certain embodiments, oligonucleotides comprise modified and/or unmodified internucleoside linkages arranged along the oligonucleotide or region thereof in a defined pattern or motif. In certain embodiments, each internucleoside linking group is a phosphodiester internucleoside linkage (P═O). In certain embodiments, each internucleoside linking group of a modified oligonucleotide is a phosphorothioate internucleoside linkage (P═S). In certain embodiments, each internucleoside linkage of a modified oligonucleotide is independently selected from a phosphorothioate internucleoside linkage and phosphodiester internucleoside linkage. In certain embodiments, each phosphorothioate internucleoside linkage is independently selected from a stereorandom phosphorothioate, a (Sp) phosphorothioate, and a (Rp) phosphorothioate. In certain embodiments, the sugar motif of a modified oligonucleotide is a gapmer and the internucleoside linkages within the gap are all modified. In certain such embodiments, some or all of the internucleoside linkages in the wings are unmodified phosphate linkages. In certain embodiments, the terminal internucleoside linkages are modified. In certain embodiments, the sugar motif of a modified oligonucleotide is a gapmer, and the internucleoside linkage motif comprises at least one phosphodiester internucleoside linkage in at least one wing, wherein the at least one phosphodiester linkage is not a terminal internucleoside linkage, and the remaining internucleoside linkages are phosphorothioate internucleoside linkages. In certain such embodiments, all of the phosphorothioate linkages are stereorandom. In certain embodiments, all of the phosphorothioate linkages in the wings are (Sp) phosphorothioates, and the gap comprises at least one Sp, Sp, Rp motif. In certain embodiments, populations of modified oligonucleotides are enriched for modified oligonucleotides comprising such internucleoside linkage motifs. 
     In certain embodiments, oligonucleotides comprise a region having an alternating internucleoside linkage motif. In certain embodiments, oligonucleotides comprise a region of uniformly modified internucleoside linkages. In certain such embodiments, the internucleoside linkages are phosphorothioate internucleoside linkages. In certain embodiments, all of the internucleoside linkages of the oligonucleotide are phosphorothioate internucleoside linkages. In certain embodiments, each internucleoside linkage of the oligonucleotide is selected from phosphodiester or phophate and phosphorothioate. In certain embodiments, each internucleoside linkage of the oligonucleotide is selected from phosphodiester or phosphate and phosphorothioate and at least one internucleoside linkage is phosphorothioate. 
     In certain embodiments, the oligonucleotide comprises at least 6 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least 8 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least 10 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least one block of at least 6 consecutive phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least one block of at least 8 consecutive phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least one block of at least 10 consecutive phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least block of at least one 12 consecutive phosphorothioate internucleoside linkages. In certain such embodiments, at least one such block is located at the 3′ end of the oligonucleotide. In certain such embodiments, at least one such block is located within 3 nucleosides of the 3′ end of the oligonucleotide. 
     In certain embodiments, oligonucleotides comprise one or more methylphosponate linkages. In certain embodiments, oligonucleotides having a gapmer nucleoside motif comprise a linkage motif comprising all phosphorothioate linkages except for one or two methylphosponate linkages. In certain embodiments, one methylphosponate linkage is in the gap of an oligonucleotide having a gapmer sugar motif. 
     In certain embodiments, it is desirable to arrange the number of phosphorothioate internucleoside linkages and phosphodiester internucleoside linkages to maintain nuclease resistance. In certain embodiments, it is desirable to arrange the number and position of phosphorothioate internucleoside linkages and the number and position of phosphodiester internucleoside linkages to maintain nuclease resistance. In certain embodiments, the number of phosphorothioate internucleoside linkages may be decreased and the number of phosphodiester internucleoside linkages may be increased. In certain embodiments, the number of phosphorothioate internucleoside linkages may be decreased and the number of phosphodiester internucleoside linkages may be increased while still maintaining nuclease resistance. In certain embodiments it is desirable to decrease the number of phosphorothioate internucleoside linkages while retaining nuclease resistance. In certain embodiments it is desirable to increase the number of phosphodiester internucleoside linkages while retaining nuclease resistance. 
     III. Certain Modified Oligonucleotides 
     In certain embodiments, compounds described herein comprise or consist of modified oligonucleotides. In certain embodiments, the above modifications (sugar, nucleobase, internucleoside linkage) are incorporated into a modified oligonucleotide. In certain embodiments, modified oligonucleotides are characterized by their modifications, motifs, and overall lengths. In certain embodiments, such parameters are each independent of one another. Thus, unless otherwise indicated, each internucleoside linkage of an oligonucleotide having a gapmer sugar motif may be modified or unmodified and may or may not follow the gapmer modification pattern of the sugar modifications. Likewise, such gapmer oligonucleotides may comprise one or more modified nucleobase independent of the gapmer pattern of the sugar modifications. Furthermore, in certain instances, an oligonucleotide is described by an overall length or range and by lengths or length ranges of two or more regions (e.g., a region of nucleosides having specified sugar modifications), in such circumstances it may be possible to select numbers for each range that result in an oligonucleotide having an overall length falling outside the specified range. In such circumstances, both elements must be satisfied. For example, in certain embodiments, a modified oligonucleotide consists of 15-20 linked nucleosides and has a sugar motif consisting of three regions or segments, A, B, and C, wherein region or segment A consists of 2-6 linked nucleosides having a specified sugar motif, region or segment B consists of 6-10 linked nucleosides having a specified sugar motif, and region or segment C consists of 2-6 linked nucleosides having a specified sugar motif. Such embodiments do not include modified oligonucleotides where A and C each consist of 6 linked nucleosides and B consists of 10 linked nucleosides (even though those numbers of nucleosides are permitted within the requirements for A, B, and C) because the overall length of such oligonucleotide is 22, which exceeds the upper limit of 20 for the overall length of the modified oligonucleotide. Unless otherwise indicated, all modifications are independent of nucleobase sequence except that the modified nucleobase 5-methylcytosine is necessarily a “C” in an oligonucleotide sequence. 
     In certain embodiments, oligonucleotides consist of X to Y linked nucleosides, where X represents the fewest number of nucleosides in the range and Y represents the largest number nucleosides in the range. In certain such embodiments, X and Y are each independently selected from 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, and 50; provided that X≤Y. For example, in certain embodiments, oligonucleotides consist of 12 to 13, 12 to 14, 12 to 15, 12 to 16, 12 to 17, 12 to 18, 12 to 19, 12 to 20, 12 to 21, 12 to 22, 12 to 23, 12 to 24, 12 to 25, 12 to 26, 12 to 27, 12 to 28, 12 to 29, 12 to 30, 13 to 14, 13 to 15, 13 to 16, 13 to 17, 13 to 18, 13 to 19, 13 to 20, 13 to 21, 13 to 22, 13 to 23, 13 to 24, 13 to 25, 13 to 26, 13 to 27, 13 to 28, 13 to 29, 13 to 30, 14 to 15, 14 to 16, 14 to 17, 14 to 18, 14 to 19, 14 to 20, 14 to 21, 14 to 22, 14 to 23, 14 to 24, 14 to 25, 14 to 26, 14 to 27, 14 to 28, 14 to 29, 14 to 30, 15 to 16, 15 to 17, 15 to 18, 15 to 19, 15 to 20, 15 to 21, 15 to 22, 15 to 23, 15 to 24, 15 to 25, 15 to 26, 15 to 27, 15 to 28, 15 to 29, 15 to 30, 16 to 17, 16 to 18, 16 to 19, 16 to 20, 16 to 21, 16 to 22, 16 to 23, 16 to 24, 16 to 25, 16 to 26, 16 to 27, 16 to 28, 16 to 29, 16 to 30, 17 to 18, 17 to 19, 17 to 20, 17 to 21, 17 to 22, 17 to 23, 17 to 24, 17 to 25, 17 to 26, 17 to 27, 17 to 28, 17 to 29, 17 to 30, 18 to 19, 18 to 20, 18 to 21, 18 to 22, 18 to 23, 18 to 24, 18 to 25, 18 to 26, 18 to 27, 18 to 28, 18 to 29, 18 to 30, 19 to 20, 19 to 21, 19 to 22, 19 to 23, 19 to 24, 19 to 25, 19 to 26, 19 to 29, 19 to 28, 19 to 29, 19 to 30, 20 to 21, 20 to 22, 20 to 23, 20 to 24, 20 to 25, 20 to 26, 20 to 27, 20 to 28, 20 to 29, 20 to 30, 21 to 22, 21 to 23, 21 to 24, 21 to 25, 21 to 26, 21 to 27, 21 to 28, 21 to 29, 21 to 30, 22 to 23, 22 to 24, 22 to 25, 22 to 26, 22 to 27, 22 to 28, 22 to 29, 22 to 30, 23 to 24, 23 to 25, 23 to 26, 23 to 27, 23 to 28, 23 to 29, 23 to 30, 24 to 25, 24 to 26, 24 to 27, 24 to 28, 24 to 29, 24 to 30, 25 to 26, 25 to 27, 25 to 28, 25 to 29, 25 to 30, 26 to 27, 26 to 28, 26 to 29, 26 to 30, 27 to 28, 27 to 29, 27 to 30, 28 to 29, 28 to 30, or 29 to 30 linked nucleosides. 
     In certain embodiments oligonucleotides have a nucleobase sequence that is complementary to a second oligonucleotide or an identified reference nucleic acid, such as a target nucleic acid. In certain embodiments, a region of an oligonucleotide has a nucleobase sequence that is complementary to a second oligonucleotide or an identified reference nucleic acid, such as a target nucleic acid. In certain embodiments, the nucleobase sequence of a region or entire length of an oligonucleotide is at least 70%, at least 80%, at least 90%, at least 95%, or 100% complementary to the second oligonucleotide or nucleic acid, such as a target nucleic acid. 
     IV. Certain Conjugated Compounds 
     In certain embodiments, the compounds described herein comprise or consist of an oligonucleotide (modified or unmodified) and optionally one or more conjugate groups and/or terminal groups. Conjugate groups consist of one or more conjugate moiety and a conjugate linker that links the conjugate moiety to the oligonucleotide. Conjugate groups may be attached to either or both ends of an oligonucleotide and/or at any internal position. In certain embodiments, conjugate groups are attached to the 2′-position of a nucleoside of a modified oligonucleotide. In certain embodiments, conjugate groups that are attached to either or both ends of an oligonucleotide are terminal groups. In certain such embodiments, conjugate groups or terminal groups are attached at the 3′ and/or 5′-end of oligonucleotides. In certain such embodiments, conjugate groups (or terminal groups) are attached at the 3′-end of oligonucleotides. In certain embodiments, conjugate groups are attached near the 3′-end of oligonucleotides. In certain embodiments, conjugate groups (or terminal groups) are attached at the 5′-end of oligonucleotides. In certain embodiments, conjugate groups are attached near the 5′-end of oligonucleotides. 
     Examples of terminal groups include but are not limited to conjugate groups, capping groups, phosphate moieties, protecting groups, modified or unmodified nucleosides, and two or more nucleosides that are independently modified or unmodified. 
     A. Certain Conjugate Groups 
     In certain embodiments, oligonucleotides are covalently attached to one or more conjugate groups. In certain embodiments, conjugate groups modify one or more properties of the attached oligonucleotide, including but not limited to pharmacodynamics, pharmacokinetics, stability, binding, absorption, tissue distribution, cellular distribution, cellular uptake, charge and clearance. In certain embodiments, conjugate groups impart a new property on the attached oligonucleotide, e.g., fluorophores or reporter groups that enable detection of the oligonucleotide. 
     Certain conjugate groups and conjugate moieties have been described previously, for example: cholesterol moiety (Letsinger et al., Proc. Natl. Acad. Sci. USA, 1989, 86, 6553-6556), cholic acid (Manoharan et al.,  Bioorg. Med. Chem. Lett.,  1994, 4, 1053-1060), a thioether, e.g., hexyl-S-tritylthiol (Manoharan et al.,  Ann. N.Y. Acad. Sci.,  1992, 660, 306-309; Manoharan et al.,  Bioorg. Med. Chem. Lett.,  1993, 3, 2765-2770), a thiocholesterol (Oberhauser et al.,  Nucl. Acids Res.,  1992, 20, 533-538), an aliphatic chain, e.g., do-decan-diol or undecyl residues (Saison-Behmoaras et al.,  EMBO J.,  1991, 10, 1111-1118; Kabanov et al.,  FEBS Lett.,  1990, 259, 327-330; Svinarchuk et al.,  Biochimie,  1993, 75, 49-54), a phospholipid, e.g., di-hexadecyl-rac-glycerol or triethyl-ammonium 1,2-di-O-hexadecyl-rac-glycero-3-H-phosphonate (Manoharan et al.,  Tetrahedron Lett.,  1995, 36, 3651-3654; Shea et al.,  Nucl. Acids Res.,  1990, 18, 3777-3783), a polyamine or a polyethylene glycol chain (Manoharan et al.,  Nucleosides  &amp;  Nucleotides,  1995, 14, 969-973), or adamantane acetic, a palmityl moiety (Mishra et al.,  Biochim. Biophys. Acta,  1995, 1264, 229-237), an octadecylamine or hexylamino-carbonyl-oxycholesterol moiety (Crooke et al.,  J. Pharmacol. Exp. Ther.,  1996, i, 923-937), a tocopherol group (Nishina et al.,  Molecular Therapy Nucleic Acids,  2015, 4, e220; doi:10.1038/mtna.2014.72 and Nishina et al.,  Molecular Therapy,  2008, 16, 734-740), or a GalNAc cluster (e.g., WO2014/179620). 
     1. Conjugate Moieties 
     Conjugate moieties include, without limitation, intercalators, reporter molecules, polyamines, polyamides, peptides, carbohydrates (e.g., GalNAc), vitamin moieties, polyethylene glycols, thioethers, polyethers, cholesterols, thiocholesterols, cholic acid moieties, folate, lipids, phospholipids, biotin, phenazine, phenanthridine, anthraquinone, adamantane, acridine, fluoresceins, rhodamines, coumarins, fluorophores, and dyes. 
     In certain embodiments, a conjugate moiety comprises an active drug substance, for example, aspirin, warfarin, phenylbutazone, ibuprofen, suprofen, fen-bufen, ketoprofen, (S)-(+)-pranoprofen, carprofen, dansylsarcosine, 2,3,5-triiodobenzoic acid, fingolimod, flufenamic acid, folinic acid, a benzothiadiazide, chlorothiazide, a diazepine, indo-methicin, a barbiturate, a cephalosporin, a sulfa drug, an antidiabetic, an antibacterial or an antibiotic. 
     2. Conjugate Linkers 
     Conjugate moieties are attached to oligonucleotides through conjugate linkers. In certain compounds, a conjugate group is a single chemical bond (i.e. conjugate moiety is attached to an oligonucleotide via a conjugate linker through a single bond). In certain embodiments, the conjugate linker comprises a chain structure, such as a hydrocarbyl chain, or an oligomer of repeating units such as ethylene glycol, nucleosides, or amino acid units. 
     In certain embodiments, a conjugate linker comprises one or more groups selected from alkyl, amino, oxo, amide, disulfide, polyethylene glycol, ether, thioether, and hydroxylamino. In certain such embodiments, the conjugate linker comprises groups selected from alkyl, amino, oxo, amide and ether groups. In certain embodiments, the conjugate linker comprises groups selected from alkyl and amide groups. In certain embodiments, the conjugate linker comprises groups selected from alkyl and ether groups. In certain embodiments, the conjugate linker comprises at least one phosphorus moiety. In certain embodiments, the conjugate linker comprises at least one phosphate group. In certain embodiments, the conjugate linker includes at least one neutral linking group. 
     In certain embodiments, conjugate linkers, including the conjugate linkers described above, are bifunctional linking moieties, e.g., those known in the art to be useful for attaching conjugate groups to parent compounds, such as the oligonucleotides provided herein. In general, a bifunctional linking moiety comprises at least two functional groups. One of the functional groups is selected to bind to a particular site on a compound and the other is selected to bind to a conjugate group. Examples of functional groups used in a bifunctional linking moiety include but are not limited to electrophiles for reacting with nucleophilic groups and nucleophiles for reacting with electrophilic groups. In certain embodiments, bifunctional linking moieties comprise one or more groups selected from amino, hydroxyl, carboxylic acid, thiol, alkyl, alkenyl, and alkynyl. 
     Examples of conjugate linkers include but are not limited to pyrrolidine, 8-amino-3,6-dioxaoctanoic acid (ADO), succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) and 6-aminohexanoic acid (AHEX or AHA). Other conjugate linkers include but are not limited to substituted or unsubstituted C 1 -C 10  alkyl, substituted or unsubstituted C 2 -C 10  alkenyl or substituted or unsubstituted C 2 -C 10  alkynyl, wherein a nonlimiting list of preferred substituent groups includes hydroxyl, amino, alkoxy, carboxy, benzyl, phenyl, nitro, thiol, thioalkoxy, halogen, alkyl, aryl, alkenyl and alkynyl. 
     In certain embodiments, conjugate linkers comprise 1-10 linker-nucleosides. In certain embodiments, such linker-nucleosides are modified nucleosides. In certain embodiments such linker-nucleosides comprise a modified sugar moiety. In certain embodiments, linker-nucleosides are unmodified. In certain embodiments, linker-nucleosides comprise an optionally protected heterocyclic base selected from a purine, substituted purine, pyrimidine or substituted pyrimidine. In certain embodiments, a cleavable moiety is a nucleoside selected from uracil, thymine, cytosine, 4-N-benzoylcytosine, 5-methylcytosine, 4-N-benzoyl-5-methylcytosine, adenine, 6-N-benzoyladenine, guanine and 2-N-isobutyrylguanine. It is typically desirable for linker-nucleosides to be cleaved from the compound after it reaches a target tissue. Accordingly, linker-nucleosides are typically linked to one another and to the remainder of the compound through cleavable bonds. In certain embodiments, such cleavable bonds are phosphodiester bonds. 
     Herein, linker-nucleosides are not considered to be part of the oligonucleotide. Accordingly, in embodiments in which a compound comprises an oligonucleotide consisting of a specified number or range of linked nucleosides and/or a specified percent complementarity to a reference nucleic acid and the compound also comprises a conjugate group comprising a conjugate linker comprising linker-nucleosides, those linker-nucleosides are not counted toward the length of the oligonucleotide and are not used in determining the percent complementarity of the oligonucleotide for the reference nucleic acid. For example, a compound may comprise (1) a modified oligonucleotide consisting of 8-30 nucleosides and (2) a conjugate group comprising 1-10 linker-nucleosides that are contiguous with the nucleosides of the modified oligonucleotide. The total number of contiguous linked nucleosides in such a compound is more than 30. Alternatively, an compound may comprise a modified oligonucleotide consisting of 8-30 nucleosides and no conjugate group. The total number of contiguous linked nucleosides in such a compound is no more than 30. Unless otherwise indicated conjugate linkers comprise no more than 10 linker-nucleosides. In certain embodiments, conjugate linkers comprise no more than 5 linker-nucleosides. In certain embodiments, conjugate linkers comprise no more than 3 linker-nucleosides. In certain embodiments, conjugate linkers comprise no more than 2 linker-nucleosides. In certain embodiments, conjugate linkers comprise no more than 1 linker-nucleoside. 
     In certain embodiments, it is desirable for a conjugate group to be cleaved from the oligonucleotide. For example, in certain circumstances compounds comprising a particular conjugate moiety are better taken up by a particular cell type, but once the compound has been taken up, it is desirable that the conjugate group be cleaved to release the unconjugated or parent oligonucleotide. Thus, certain conjugate may comprise one or more cleavable moieties, typically within the conjugate linker. In certain embodiments, a cleavable moiety is a cleavable bond. In certain embodiments, a cleavable moiety is a group of atoms comprising at least one cleavable bond. In certain embodiments, a cleavable moiety comprises a group of atoms having one, two, three, four, or more than four cleavable bonds. In certain embodiments, a cleavable moiety is selectively cleaved inside a cell or subcellular compartment, such as a lysosome. In certain embodiments, a cleavable moiety is selectively cleaved by endogenous enzymes, such as nucleases. 
     In certain embodiments, a cleavable bond is selected from among: an amide, an ester, an ether, one or both esters of a phosphodiester, a phosphate ester, a carbamate, or a disulfide. In certain embodiments, a cleavable bond is one or both of the esters of a phosphodiester. In certain embodiments, a cleavable moiety comprises a phosphate or phosphodiester. In certain embodiments, the cleavable moiety is a phosphate linkage between an oligonucleotide and a conjugate moiety or conjugate group. 
     In certain embodiments, a cleavable moiety comprises or consists of one or more linker-nucleosides. In certain such embodiments, one or more linker-nucleosides are linked to one another and/or to the remainder of the compound through cleavable bonds. In certain embodiments, such cleavable bonds are unmodified phosphodiester bonds. In certain embodiments, a cleavable moiety is 2′-deoxy nucleoside that is attached to either the 3′ or 5′-terminal nucleoside of an oligonucleotide by a phosphate internucleoside linkage and covalently attached to the remainder of the conjugate linker or conjugate moiety by a phosphate or phosphorothioate linkage. In certain such embodiments, the cleavable moiety is 2′-deoxyadenosine. 
     3. Certain Cell-Targeting Conjugate Moieties 
     In certain embodiments, a conjugate group comprises a cell-targeting conjugate moiety. In certain embodiments, a conjugate group has the general formula: 
     
       
         
         
             
             
         
       
         
         
           
             wherein n is from 1 to about 3, m is 0 when n is 1, m is 1 when n is 2 or greater, j is 1 or 0, and k is 1 or 0. 
           
         
       
    
     In certain embodiments, n is 1, j is 1 and k is 0. In certain embodiments, n is 1, j is 0 and k is 1. In certain embodiments, n is 1, j is 1 and k is 1. In certain embodiments, n is 2, j is 1 and k is 0. In certain embodiments, n is 2, j is 0 and k is 1. In certain embodiments, n is 2, j is 1 and k is 1. In certain embodiments, n is 3, j is 1 and k is 0. In certain embodiments, n is 3, j is 0 and k is 1. In certain embodiments, n is 3, j is 1 and k is 1. 
     In certain embodiments, conjugate groups comprise cell-targeting moieties that have at least one tethered ligand. In certain embodiments, cell-targeting moieties comprise two tethered ligands covalently attached to a branching group. In certain embodiments, cell-targeting moieties comprise three tethered ligands covalently attached to a branching group. 
     In certain embodiments, the cell-targeting moiety comprises a branching group comprising one or more groups selected from alkyl, amino, oxo, amide, disulfide, polyethylene glycol, ether, thioether and hydroxylamino groups. In certain embodiments, the branching group comprises a branched aliphatic group comprising groups selected from alkyl, amino, oxo, amide, disulfide, polyethylene glycol, ether, thioether and hydroxylamino groups. In certain such embodiments, the branched aliphatic group comprises groups selected from alkyl, amino, oxo, amide and ether groups. In certain such embodiments, the branched aliphatic group comprises groups selected from alkyl, amino and ether groups. In certain such embodiments, the branched aliphatic group comprises groups selected from alkyl and ether groups. In certain embodiments, the branching group comprises a mono or polycyclic ring system. 
     In certain embodiments, each tether of a cell-targeting moiety comprises one or more groups selected from alkyl, substituted alkyl, ether, thioether, disulfide, amino, oxo, amide, phosphodiester, and polyethylene glycol, in any combination. In certain embodiments, each tether is a linear aliphatic group comprising one or more groups selected from alkyl, ether, thioether, disulfide, amino, oxo, amide, and polyethylene glycol, in any combination. In certain embodiments, each tether is a linear aliphatic group comprising one or more groups selected from alkyl, phosphodiester, ether, amino, oxo, and amide, in any combination. In certain embodiments, each tether is a linear aliphatic group comprising one or more groups selected from alkyl, ether, amino, oxo, and amid, in any combination. In certain embodiments, each tether is a linear aliphatic group comprising one or more groups selected from alkyl, amino, and oxo, in any combination. In certain embodiments, each tether is a linear aliphatic group comprising one or more groups selected from alkyl and oxo, in any combination. In certain embodiments, each tether is a linear aliphatic group comprising one or more groups selected from alkyl and phosphodiester, in any combination. In certain embodiments, each tether comprises at least one phosphorus linking group or neutral linking group. In certain embodiments, each tether comprises a chain from about 6 to about 20 atoms in length. In certain embodiments, each tether comprises a chain from about 10 to about 18 atoms in length. In certain embodiments, each tether comprises about 10 atoms in chain length. 
     In certain embodiments, each ligand of a cell-targeting moiety has an affinity for at least one type of receptor on a target cell. In certain embodiments, each ligand has an affinity for at least one type of receptor on the surface of a mammalian lung cell. 
     In certain embodiments, each ligand of a cell-targeting moiety is a carbohydrate, carbohydrate derivative, modified carbohydrate, polysaccharide, modified polysaccharide, or polysaccharide derivative. In certain such embodiments, the conjugate group comprises a carbohydrate cluster (see, e.g., Maier et al., “Synthesis of Antisense Oligonucleotides Conjugated to a Multivalent Carbohydrate Cluster for Cellular Targeting,”  Bioconjugate Chemistry,  2003, 14, 18-29, or Rensen et al., “Design and Synthesis of Novel N-Acetylgalactosamine-Terminated Glycolipids for Targeting of Lipoproteins to the Hepatic Asiaglycoprotein Receptor,”  J. Med. Chem.  2004, 47, 5798-5808, which are incorporated herein by reference in their entirety). In certain such embodiments, each ligand is an amino sugar or a thio sugar. For example, amino sugars may be selected from any number of compounds known in the art, such as sialic acid, α-D-galactosamine, β-muramic acid, 2-deoxy-2-methylamino-L-glucopyranose, 4,6-dideoxy-4-formamido-2,3-di-O-methyl-D-mannopyranose, 2-deoxy-2-sulfoamino-D-glucopyranose and N-sulfo-D-glucosamine, and N-glycoloyl-α-neuraminic acid. For example, thio sugars may be selected from 5-Thio-β-D-glucopyranose, methyl 2,3,4-tri-O-acetyl-1-thio-6-O-trityl-α-D-glucopyranoside, 4-thio-β-D-galactopyranose, and ethyl 3,4,6,7-tetra-O-acetyl-2-deoxy-1,5-dithio-α-D-gluco-heptopyranoside. 
     In certain embodiments compounds described herein comprise a conjugate group found in any of the following references: Lee,  Carbohydr Res,  1978, 67, 509-514; Connolly et al.,  J Biol Chem,  1982, 257, 939-945; Pavia et al.,  Int J Pep Protein Res,  1983, 22, 539-548; Lee et al.,  Biochem,  1984, 23, 4255-4261; Lee et al.,  Glycoconjugate J,  1987, 4, 317-328; Toyokuni et al.,  Tetrahedron Lett,  1990, 31, 2673-2676; Biessen et al.,  J Med Chem,  1995, 38, 1538-1546; Valentijn et al.,  Tetrahedron,  1997, 53, 759-770; Kim et al.,  Tetrahedron Lett,  1997, 38, 3487-3490; Lee et al.,  Bioconjug Chem,  1997, 8, 762-765; Kato et al.,  Glycobiol,  2001, 11, 821-829; Rensen et al.,  J Biol Chem,  2001, 276, 37577-37584; Lee et al.,  Methods Enzymol,  2003, 362, 38-43; Westerlind et al.,  Glycoconj J,  2004, 21, 227-241; Lee et al.,  Bioorg Med Chem Lett,  2006, 16(19), 5132-5135; Maierhofer et al.,  Bioorg Med Chem,  2007, 15, 7661-7676; Khorev et al.,  Bioorg Med Chem,  2008, 16, 5216-5231; Lee et al.,  Bioorg Med Chem,  2011, 19, 2494-2500; Kornilova et al.,  Analyt Biochem,  2012, 425, 43-46; Pujol et al.,  Angew Chemie Int Ed Engl,  2012, 51, 7445-7448; Biessen et al.,  J Med Chem,  1995, 38, 1846-1852; Sliedregt et al.,  J Med Chem,  1999, 42, 609-618; Rensen et al.,  J Med Chem,  2004, 47, 5798-5808; Rensen et al.,  Arterioscler Thromb Vasc Biol,  2006, 26, 169-175; van Rossenberg et al.,  Gene Ther,  2004, 11, 457-464; Sato et al.,  J Am Chem Soc,  2004, 126, 14013-14022; Lee et al.,  J Org Chem,  2012, 77, 7564-7571; Biessen et al.,  FASEB J,  2000, 14, 1784-1792; Rajur et al.,  Bioconjug Chem,  1997, 8, 935-940; Duff et al.,  Methods Enzymol,  2000, 313, 297-321; Maier et al.,  Bioconjug Chem,  2003, 14, 18-29; Jayaprakash et al.,  Org Lett,  2010, 12, 5410-5413; Manoharan,  Antisense Nucleic Acid Drug Dev,  2002, 12, 103-128; Merwin et al.,  Bioconjug Chem,  1994, 5, 612-620; Tomiya et al., Bioorg Med Chem, 2013, 21, 5275-5281; International applications WO1998/013381; WO2011/038356; WO1997/046098; WO2008/098788; WO2004/101619; WO2012/037254; WO2011/120053; WO2011/100131; WO2011/163121; WO2012/177947; WO2013/033230; WO2013/075035; WO2012/083185; WO2012/083046; WO2009/082607; WO2009/134487; WO2010/144740; WO2010/148013; WO1997/020563; WO2010/088537; WO2002/043771; WO2010/129709; WO2012/068187; WO2009/126933; WO2004/024757; WO2010/054406; WO2012/089352; WO2012/089602; WO2013/166121; WO2013/165816; U.S. Pat. Nos. 4,751,219; 8,552,163; 6,908,903; 7,262,177; 5,994,517; 6,300,319; 8,106,022; 7,491,805; 7,491,805; 7,582,744; 8,137,695; 6,383,812; 6,525,031; 6,660,720; 7,723,509; 8,541,548; 8,344,125; 8,313,772; 8,349,308; 8,450,467; 8,501,930; 8,158,601; 7,262,177; 6,906,182; 6,620,916; 8,435,491; 8,404,862; 7,851,615; Published U.S. Patent Application Publications US2011/0097264; US2011/0097265; US2013/0004427; US2005/0164235; US2006/0148740; US2008/0281044; US2010/0240730; US2003/0119724; US2006/0183886; US2008/0206869; US2011/0269814; US2009/0286973; US2011/0207799; US2012/0136042; US2012/0165393; US2008/0281041; US2009/0203135; US2012/0035115; US2012/0095075; US2012/0101148; US2012/0128760; US2012/0157509; US2012/0230938; US2013/0109817; US2013/0121954; US2013/0178512; US2013/0236968; US2011/0123520; US2003/0077829; US2008/0108801; and US2009/0203132. 
     Compositions and Methods for Formulating Pharmaceutical Compositions 
     Compounds described herein may be admixed with pharmaceutically acceptable active or inert substances for the preparation of pharmaceutical compositions. Compositions and methods for the formulation of pharmaceutical compositions are dependent upon a number of criteria, including, but not limited to, route of administration, extent of disease, or dose to be administered. 
     Certain embodiments provide pharmaceutical compositions comprising one or more compounds or a salt thereof. In certain embodiments, the compounds are antisense compounds or oligomeric compounds. In certain embodiments, the compounds comprise or consist of a modified oligonucleotide. In certain such embodiments, the pharmaceutical composition comprises a suitable pharmaceutically acceptable diluent or carrier. In certain embodiments, a pharmaceutical composition comprises a sterile saline solution and one or more compound. In certain embodiments, such pharmaceutical composition consists of a sterile saline solution and one or more compound. In certain embodiments, the sterile saline is pharmaceutical grade saline. In certain embodiments, a pharmaceutical composition comprises one or more compound and sterile water. In certain embodiments, a pharmaceutical composition consists of one compound and sterile water. In certain embodiments, the sterile water is pharmaceutical grade water. In certain embodiments, a pharmaceutical composition comprises or consists of one or more compound and phosphate-buffered saline (PBS). In certain embodiments, a pharmaceutical composition consists of one or more compound and sterile PBS. In certain embodiments, the sterile PBS is pharmaceutical grade PBS. Compositions and methods for the formulation of pharmaceutical compositions are dependent upon a number of criteria, including, but not limited to, route of administration, extent of disease, or dose to be administered. 
     Certain embodiments provide pharmaceutical compositions suitable for aerosolization and/or dispersal by a nebulizer or inhaler. Such devices are well known in the art. In certain such embodiments, the pharmaceutical composition is a solid comprising particles of compounds that are of respirable size. A solid particulate composition can optionally contain a dispersant which serves to facilitate the formation of an aerosol, e.g., lactose. Solid pharmaceutical compositions comprising an oligonucleotide can also be aerosolized using any solid particulate medicament aerosol generator known in the art, e.g., a dry powder inhaler. In certain embodiments, the powder employed in the inhaler consists of the compound comprising the active compound or of a powder blend comprising the active compound, a suitable powder diluent, and an optional surfactant. 
     In certain embodiments, the pharmaceutical composition is a liquid. In certain such embodiments, the liquid is administered as an aerosol that is produced by any suitable means, such as with a nebulizer or inhaler. See, e.g., U.S. Pat. No. 4,501,729. Nebulizers are devices that transform solutions or suspensions into an aerosol mist and are well known in the art. Suitable nebulizers include jet nebulizers, ultrasonic nebulizers, electronic mesh nebulizers, and vibrating mesh nebulizers. Companies such as PART and Vectura sell some types of such suitable nebulziers. In certain embodiments, the aerosol is produced by a metered dose inhaler, which typically contains a suspension or solution formulation of the active compound in a liquefied propellant. Inhalers suitable for dispensing liquid aerosol also include certain inhalers sold by Respimat (See, e.g., Anderson,  Int J Chron Obstruct Pulmon Dis.  1, 251 (2006).) Pharmaceutical compositions suitable for aerosolization can comprise propellants, surfactants, co-solvents, dispersants, preservatives, and/or other additives or excipients. 
     A compound described herein complementary to an α-ENaC nucleic acid can be utilized in pharmaceutical compositions by combining the compound with a suitable pharmaceutically acceptable diluent or carrier and/or additional components such that the pharmaceutical composition is suitable for aerosolization by a nebulizer. In certain embodiments, a pharmaceutically acceptable diluent is phosphate buffered saline. Accordingly, in one embodiment, employed in the methods described herein is a pharmaceutical composition comprising a compound complementary to an α-ENaC nucleic acid and a pharmaceutically acceptable diluent. In certain embodiments, the pharmaceutically acceptable diluent is phosphate buffered saline. In certain embodiments, the compound comprises or consists of a modified oligonucleotide provided herein. 
     Pharmaceutical compositions comprising compounds provided herein encompass any pharmaceutically acceptable salts, esters, or salts of such esters, or any other oligonucleotide which, upon administration to an animal, including a human, is capable of providing (directly or indirectly) the biologically active metabolite or residue thereof. In certain embodiments, the compounds are antisense compounds or oligomeric compounds. In certain embodiments, the compound comprises or consists of a modified oligonucleotide. Accordingly, for example, the disclosure is also drawn to pharmaceutically acceptable salts of compounds, prodrugs, pharmaceutically acceptable salts of such prodrugs, and other bioequivalents. Suitable pharmaceutically acceptable salts include, but are not limited to, sodium and potassium salts. 
     A prodrug can include the incorporation of additional nucleosides at one or both ends of a compound which are cleaved by endogenous nucleases within the body, to form the active compound. In certain embodiments, the compounds or compositions further comprise a pharmaceutically acceptable carrier or diluent. 
     Examples 
     The Examples below describe the screening process used to identify lead compounds targeted to α-ENaC. Out of over 1,900 oligonucleotides that were screened, many potent and tolerable oligonucleotides were identified, and compounds 797308, 797495, 826763, 827307, 827359, and 827392 emerged as the top lead compounds. In particular, compound 827359 exhibited the best combination of properties in terms of potency and tolerability. 
     Non-Limiting Disclosure and Incorporation by Reference 
     Although the sequence listing accompanying this filing identifies each sequence as either “RNA” or “DNA” as required, in reality, those sequences may be modified with any combination of chemical modifications. One of skill in the art will readily appreciate that such designation as “RNA” or “DNA” to describe modified oligonucleotides is, in certain instances, arbitrary. For example, an oligonucleotide comprising a nucleoside comprising a 2′-OH sugar moiety and a thymine nucleobase could be described as a DNA having an RNA sugar, or as an RNA having a DNA nucleobase. 
     Accordingly, nucleic acid sequences provided herein, including, but not limited to those in the sequence listing, are intended to encompass nucleic acids containing any combination of unmodified or modified RNA and/or DNA, including, but not limited to such nucleic acids having modified nucleobases. By way of further example and without limitation, an oligonucleotide having the nucleobase sequence “ATCGATCG” encompasses any oligonucleotides having such nucleobase sequence, whether modified or unmodified, including, but not limited to, such compounds comprising RNA bases, such as those having sequence “AUCGAUCG” and those having some DNA bases and some RNA bases such as “AUCGATCG” and compounds having other modified nucleobases, such as “AT m CGAUCG,” wherein  m C indicates a cytosine base comprising a methyl group at the 5-position. 
     While certain compounds, compositions and methods described herein have been described with specificity in accordance with certain embodiments, the following examples serve only to illustrate the compounds described herein and are not intended to limit the same. Each of the references recited in the present application is incorporated herein by reference in its entirety. 
     Example 1: Effect of Modified Oligonucleotides Complementary to α-ENaC In Vitro 
     Modified oligonucleotides complementary to one or more human α-ENaC nucleic acids were designed and tested for their effect on α-ENaC mRNA in vitro. The modified oligonucleotides were tested in a series of experiments that had similar culture conditions. 
     Cultured Hep3B cells at a density of 20,000 cells per well were transfected using electroporation with 2,000 nM of modified oligonucleotide or no modified oligonucleotide for untreated controls. After approximately 24 hours, RNA was isolated from the cells and α-ENaC mRNA levels were measured by quantitative real-time PCR. Human primer probe set hSCNN1A_LTS01170 (forward sequence ACATCCCAGGAATGGGTCTTC, designated herein as SEQ ID NO: 3; reverse sequence ACTTTGGCCACTCCATTTCTCTT, designated herein as SEQ ID NO: 4; probe sequence TGCTATCGCGACAGAACAATTACACCGTC, designated herein as SEQ ID: 5) was used to measure mRNA levels. α-ENaC mRNA levels were normalized to total RNA content, as measured by RIBOGREEN®. Results are presented in the tables below as normalized α-ENaC mRNA level, relative to untreated control cells (these conditions describe a “Standard Cell Assay”). 
     The modified oligonucleotides in the tables below each have a 3-10-3 phosphothiorate cEt gapmer motif. The modified oligonucleotides are 16 nuceobases in length, wherein the central gap segment contains ten 2′-deoxynucleosides and is flanked by wing segments on the 3′ and 5′ ends, each containing three cEt nucleosides. All cytosine residues throughout each modified oligonucletoide are 5-methyl cytosines. The internucleoside linkages are all phosphorothioate internucleoside linkages. 
     Each modified oligonucleotide listed in the tables below is 100% complementary to the human α-ENaC nucleic acid sequence of GenBank Number NM_001038.5 (designated herein as SEQ ID NO: 1), the complement of GenBank Number NC_000012.12, truncated from nucleosides 6343001 to 6380000 (designated herein as SEQ ID NO: 2), and/or GenBank Number NG_011945.1 (designated herein as SEQ ID NO: 1957). “Start Site” indicates the 5′-most nucleoside of the designated α-ENaC nucleic acid to which the oligonucleotide is complementary. “Stop Site” indicates the 3′-most nucleoside of the human α-ENaC nucleic acid to which the oligonucleotide is complementary. ‘N/A’ indicates that the modified oligonucleotide is not complementary to that particular nucleic acid with 100% complementarity. Several oligonucleotides match two or more sites on the mRNA, as shown in the tables below. As shown below, modified oligonucleotides complementary to human α-ENaC reduced the amount of human α-ENaC mRNA in vitro. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 SEQ ID: 1 
                 SEQ ID: 1 
                   
                 α-ENaC 
                 SEQ ID: 2 
                 SEQ ID 2: 
                   
               
               
                 Compound 
                 Start 
                 Stop 
                   
                 (% 
                 Start 
                 Stop 
                 SEQ ID 
               
               
                 Number 
                 Site 
                 Site 
                 Sequence 
                 control) 
                 Site 
                 Site 
                 NO 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 668181 
                 523 
                 538 
                 CTTCATGCGGTTGTGC 
                 37 
                 5451 
                 5466 
                 6 
               
               
                   
               
               
                 668248 
                 1240 
                 1255 
                 AGGCATGGAAGACATC 
                 94 
                 24196 
                 24211 
                 7 
               
               
                   
               
               
                 668279 
                 1575 
                 1590 
                 ATGTAGGCACAGCCAC 
                 30 
                 25489 
                 25504 
                 8 
               
               
                   
               
               
                 668280 
                 1580 
                 1595 
                 AGAAGATGTAGGCACA 
                 27 
                 25494 
                 25509 
                 9 
               
               
                   
               
               
                 668324 
                 1930 
                 1945 
                 GCCCAGGTTGGACAGG 
                 54 
                 31759 
                 31774 
                 10 
               
               
                   
               
               
                 668325 
                 1954 
                 1969 
                 GCCGAACCACAGGCTC 
                 72 
                 31783 
                 31798 
                 11 
               
               
                   
               
               
                 668358 
                 2599 
                 2614 
                 TGTCAAAGCTCCAAGT 
                 43 
                 32428 
                 32443 
                 12 
               
               
                   
               
               
                 668364 
                 2766 
                 2781 
                 ACCCAAGTTCAAGAGG 
                 76 
                 32595 
                 32610 
                 13 
               
               
                   
               
               
                 797074 
                 4 
                 19 
                 TTAGACGCAGACAGGC 
                 73 
                 4265 
                 4280 
                 14 
               
               
                   
               
               
                 797075 
                 30 
                 45 
                 GAGAAGGCGGACTCTG 
                 88 
                 4291 
                 4306 
                 15 
               
               
                   
               
               
                 797076 
                 43 
                 58 
                 GAGTACTGGACCTGAG 
                 64 
                 4304 
                 4319 
                 16 
               
               
                   
               
               
                 797077 
                 51 
                 66 
                 TGAACTGGGAGTACTG 
                 71 
                 4312 
                 4327 
                 17 
               
               
                   
               
               
                 797078 
                 63 
                 78 
                 CCCGAGGGCAGGTGAA 
                 97 
                 4324 
                 4339 
                 18 
               
               
                   
               
               
                 797079 
                 75 
                 90 
                 GGAAGGAGGGCTCCCG 
                 98 
                 4336 
                 4351 
                 19 
               
               
                   
               
               
                 797080 
                 82 
                 97 
                 TTCCGAAGGAAGGAGG 
                 123 
                 4343 
                 4358 
                 20 
               
               
                   
               
               
                 797081 
                 90 
                 105 
                 CGGGAGTTTTCCGAAG 
                 135 
                 4351 
                 4366 
                 21 
               
               
                   
               
               
                 797082 
                 97 
                 112 
                 TCAGAGCCGGGAGTTT 
                 64 
                 4358 
                 4373 
                 22 
               
               
                   
               
               
                 797083 
                 110 
                 125 
                 GGCTGAGGAGGAGTCA 
                 86 
                 4371 
                 4386 
                 23 
               
               
                   
               
               
                 797084 
                 135 
                 150 
                 TAAAGGTGAGCAGGGC 
                 107 
                 4396 
                 4411 
                 24 
               
               
                   
               
               
                 797085 
                 137 
                 152 
                 ATTAAAGGTGAGCAGG 
                 96 
                 4398 
                 4413 
                 25 
               
               
                   
               
               
                 797086 
                 139 
                 154 
                 CAATTAAAGGTGAGCA 
                 137 
                 4400 
                 4415 
                 26 
               
               
                   
               
               
                 797087 
                 141 
                 156 
                 CTCAATTAAAGGTGAG 
                 150 
                 4402 
                 4417 
                 27 
               
               
                   
               
               
                 797088 
                 142 
                 157 
                 TCTCAATTAAAGGTGA 
                 73 
                 4403 
                 4418 
                 28 
               
               
                   
               
               
                 797089 
                 147 
                 162 
                 TAGCATCTCAATTAAA 
                 61 
                 4408 
                 4423 
                 29 
               
               
                   
               
               
                 797090 
                 151 
                 166 
                 TCATTAGCATCTCAAT 
                 104 
                 4412 
                 4427 
                 30 
               
               
                   
               
               
                 797091 
                 158 
                 173 
                 AGGAATCTCATTAGCA 
                 70 
                 4419 
                 4434 
                 31 
               
               
                   
               
               
                 797092 
                 168 
                 183 
                 TGGAAGCGACAGGAAT 
                 94 
                 4429 
                 4444 
                 32 
               
               
                   
               
               
                 797093 
                 177 
                 192 
                 GGCCAGGGATGGAAGC 
                 100 
                 4438 
                 4453 
                 33 
               
               
                   
               
               
                 797094 
                 213 
                 228 
                 GTGCAGCGGCCTGGCT 
                 73 
                 4474 
                 4489 
                 34 
               
               
                   
               
               
                 797095 
                 221 
                 236 
                 CCTGACAGGTGCAGCG 
                 71 
                 4482 
                 4497 
                 35 
               
               
                   
               
               
                 797096 
                 235 
                 250 
                 CTCCAGCTTGTTCCC 
                 41 
                 5163 
                 5178 
                 36 
               
               
                   
                 295 
                 310 
                   
                   
                 5223 
                 5238 
                   
               
               
                   
               
               
                 797097 
                 237 
                 252 
                 TCCTCCAGCTTGTTCC 
                 49 
                 5165 
                 5180 
                 37 
               
               
                   
                 297 
                 312 
                   
                   
                 5225 
                 5240 
                   
               
               
                   
               
               
                 797098 
                 238 
                 253 
                 CTCCTCCAGCTTGTTC 
                 58 
                 5166 
                 5181 
                 38 
               
               
                   
                 298 
                 313 
                   
                   
                 5226 
                 5241 
                   
               
               
                   
               
               
                 797099 
                 240 
                 255 
                 TGCTCCTCCAGCTTGT 
                 26 
                 5168 
                 5183 
                 39 
               
               
                   
                 300 
                 315 
                   
                   
                 5228 
                 5243 
                   
               
               
                   
               
               
                 797100 
                 242 
                 257 
                 CCTGCTCCTCCAGCTT 
                 19 
                 5170 
                 5185 
                 40 
               
               
                   
                 302 
                 317 
                   
                   
                 5230 
                 5245 
                   
               
               
                   
               
               
                 797101 
                 244 
                 259 
                 GTCCTGCTCCTCCAGC 
                 21 
                 5172 
                 5187 
                 41 
               
               
                   
                 304 
                 319 
                   
                   
                 5232 
                 5247 
                   
               
               
                   
               
               
                 797102 
                 251 
                 266 
                 GTCTAGGGTCCTGCTC 
                 41 
                 5179 
                 5194 
                 42 
               
               
                   
               
               
                 797103 
                 258 
                 273 
                 TGCAGAGGTCTAGGGT 
                 27 
                 5186 
                 5201 
                 43 
               
               
                   
               
               
                 797104 
                 268 
                 283 
                 TGGTATGGGCTGCAGA 
                 18 
                 5196 
                 5211 
                 44 
               
               
                   
               
               
                 797105 
                 277 
                 292 
                 CATGAGACCTGGTATG 
                 85 
                 5205 
                 5220 
                 45 
               
               
                   
               
               
                 797106 
                 311 
                 326 
                 GGCTAGAGTCCTGCTC 
                 78 
                 5239 
                 5254 
                 46 
               
               
                   
               
               
                 797107 
                 329 
                 344 
                 CTGGAGTGGACTGTGG 
                 73 
                 5257 
                 5272 
                 47 
               
               
                   
               
               
                 797108 
                 403 
                 418 
                 CGCCGTGGGCTGCTGG 
                 60 
                 5331 
                 5346 
                 48 
               
               
                   
               
               
                 797109 
                 424 
                 439 
                 CTCGATCAGGGCCTCC 
                 33 
                 5352 
                 5367 
                 49 
               
               
                   
               
               
                 797110 
                 438 
                 453 
                 TAGGAGCGGTGGAACT 
                 51 
                 5366 
                 5381 
                 50 
               
               
                   
               
               
                 797111 
                 440 
                 455 
                 GGTAGGAGCGGTGGAA 
                 32 
                 5368 
                 5383 
                 51 
               
               
                   
               
               
                 797112 
                 447 
                 462 
                 AGCTCTCGGTAGGAGC 
                 84 
                 5375 
                 5390 
                 52 
               
               
                   
               
               
                 797113 
                 454 
                 469 
                 CTCGAAGAGCTCTCGG 
                 34 
                 5382 
                 5397 
                 53 
               
               
                   
               
               
                 797114 
                 462 
                 477 
                 CAGAAGAACTCGAAGA 
                 48 
                 5390 
                 5405 
                 54 
               
               
                   
               
               
                 797115 
                 534 
                 549 
                 CAGAAGGCCGTCTTCA 
                 30 
                 5462 
                 5477 
                 55 
               
               
                   
               
               
                 797116 
                 537 
                 552 
                 GCCCAGAAGGCCGTCT 
                 41 
                 5465 
                 5480 
                 56 
               
               
                   
               
               
                 797117 
                 554 
                 569 
                 TGCAGAGCCACAGCAC 
                 47 
                 5482 
                 5497 
                 57 
               
               
                   
               
               
                 797118 
                 561 
                 576 
                 CCAAAGGTGCAGAGCC 
                 17 
                 5489 
                 5504 
                 58 
               
               
                   
               
               
                 797119 
                 568 
                 583 
                 CATCATGCCAAAGGTG 
                 26 
                 5496 
                 5511 
                 59 
               
               
                   
               
               
                 797120 
                 576 
                 591 
                 TGCCAGTACATCATGC 
                 45 
                 5504 
                 5519 
                 60 
               
               
                   
               
               
                 797121 
                 583 
                 598 
                 GCCGAATTGCCAGTAC 
                 20 
                 5511 
                 5526 
                 61 
               
               
                   
               
               
                 797122 
                 592 
                 607 
                 GAAAAGCAGGCCGAAT 
                 49 
                 5520 
                 5535 
                 62 
               
               
                   
               
               
                 797123 
                 604 
                 619 
                 GAAGTACTCTCCGAAA 
                 36 
                 5532 
                 5547 
                 63 
               
               
                   
               
               
                 797124 
                 642 
                 657 
                 TCCGAGTTGAGGTTGA 
                 25 
                 5570 
                 5585 
                 64 
               
               
                   
               
               
                 797125 
                 651 
                 666 
                 ACGAGCTTGTCCGAGT 
                 23 
                 5579 
                 5594 
                 65 
               
               
                   
               
               
                 797126 
                 682 
                 697 
                 ATTGAGGGTGCAGATG 
                 58 
                 5610 
                 5625 
                 66 
               
               
                   
               
               
                 797127 
                 704 
                 719 
                 TAATTTCCGGGTACCT 
                 30 
                 16289 
                 16304 
                 67 
               
               
                   
               
               
                 797128 
                 736 
                 751 
                 TGTGATGCGGTCCAGC 
                 24 
                 16321 
                 16336 
                 68 
               
               
                   
               
               
                 797129 
                 760 
                 775 
                 GTACAGGTCAAAGAGC 
                 20 
                 16345 
                 16360 
                 69 
               
               
                   
               
               
                 797130 
                 765 
                 780 
                 TATTTGTACAGGTCAA 
                 20 
                 16350 
                 16365 
                 70 
               
               
                   
               
               
                 797131 
                 767 
                 782 
                 TGTATTTGTACAGGTC 
                 13 
                 16352 
                 16367 
                 71 
               
               
                   
               
               
                 797132 
                 778 
                 793 
                 GGTGAAGGAGCTGTAT 
                 35 
                 16363 
                 16378 
                 72 
               
               
                   
               
               
                 797133 
                 785 
                 800 
                 CGAGAGTGGTGAAGGA 
                 72 
                 16370 
                 16385 
                 73 
               
               
                   
               
               
                 797134 
                 793 
                 808 
                 GCCGGCCACGAGAGTG 
                 101 
                 16378 
                 16393 
                 74 
               
               
                   
               
               
                 797135 
                 802 
                 817 
                 GCTGCGGGAGCCGGCC 
                 59 
                 16387 
                 16402 
                 75 
               
               
                   
               
               
                 797136 
                 809 
                 824 
                 CGCGACGGCTGCGGGA 
                 38 
                 16394 
                 16409 
                 76 
               
               
                   
               
               
                 797137 
                 817 
                 832 
                 CCGCAGGTCGCGACGG 
                 74 
                 16402 
                 16417 
                 77 
               
               
                   
               
               
                 797138 
                 880 
                 895 
                 TCGACGGGCCCCGTGA 
                 41 
                 16465 
                 16480 
                 78 
               
               
                   
               
               
                 797139 
                 890 
                 905 
                 CGCTACGGGCTCGACG 
                 20 
                 16475 
                 16490 
                 79 
               
               
                   
               
               
                 797140 
                 901 
                 916 
                 GCTGGAGGCCACGCTA 
                 21 
                 16486 
                 16501 
                 80 
               
               
                   
               
               
                 797141 
                 942 
                 957 
                 TTCCAGTCCTTCCAGT 
                 45 
                 16527 
                 16542 
                 81 
               
               
                   
               
               
                 797142 
                 950 
                 965 
                 AGCCGATCTTCCAGTC 
                 44 
                 16535 
                 16550 
                 82 
               
               
                   
               
               
                 797143 
                 961 
                 976 
                 GCACAGCTGGAAGCCG 
                 30 
                 N/A 
                 N/A 
                 83 
               
               
                   
               
               
                 797144 
                 977 
                 992 
                 CCGATTTGTTCTGGTT 
                 19 
                 17763 
                 17778 
                 84 
               
               
                   
               
               
                 797145 
                 984 
                 999 
                 AAGCAGTCCGATTTGT 
                 74 
                 17770 
                 17785 
                 85 
               
               
                   
               
               
                 797146 
                 1002 
                 1017 
                 GATGAGTATGTCTGGT 
                 18 
                 17788 
                 17803 
                 86 
               
               
                   
               
               
                 797147 
                 1016 
                 1031 
                 CCGCATCCACCCCTGA 
                 27 
                 17802 
                 17817 
                 87 
               
               
                   
               
               
                 797148 
                 1044 
                 1059 
                 ATGTAGTGGAAGCGGT 
                 59 
                 17830 
                 17845 
                 88 
               
               
                   
               
               
                 797149 
                 1057 
                 1072 
                 CGACAGGATGTTGATG 
                 45 
                 17843 
                 17858 
                 89 
               
               
                   
               
               
                 797150 
                 1066 
                 1081 
                 TGGCAGCCTCGACAGG 
                 24 
                 17852 
                 17867 
                 90 
               
               
                   
               
               
                 797151 
                 1077 
                 1092 
                 GGCAGAGTCTCTGGCA 
                 39 
                 17863 
                 17878 
                 91 
               
               
                   
               
               
                 797152 
                 1087 
                 1102 
                 CTCCAGGGATGGCAGA 
                 60 
                 17873 
                 17888 
                 92 
               
               
                   
               
               
                 797153 
                 1111 
                 1126 
                 GATGAAGTTGCCCAGC 
                 24 
                 17897 
                 17912 
                 93 
               
               
                   
               
               
                 797154 
                 1118 
                 1133 
                 AGGCGAAGATGAAGTT 
                 51 
                 17904 
                 17919 
                 94 
               
               
                   
               
               
                 797155 
                 1128 
                 1143 
                 TTGAAGCGGCAGGCGA 
                 48 
                 17914 
                 17929 
                 95 
               
               
                   
               
               
                 797156 
                 1143 
                 1158 
                 TTGCAGGAGACCTGGT 
                 45 
                 17929 
                 17944 
                 96 
               
               
                   
               
               
                 797157 
                 1156 
                 1171 
                 GTAATTCGCCTGGTTG 
                 102 
                 N/A 
                 N/A 
                 97 
               
               
                   
               
               
                 797158 
                 1163 
                 1178 
                 AGTGAGAGTAATTCGC 
                 53 
                 N/A 
                 N/A 
                 98 
               
               
                   
               
               
                 797159 
                 1227 
                 1242 
                 ATCCAGAGGTTGGAGT 
                 105 
                 24183 
                 24198 
                 99 
               
               
                   
               
               
                 797160 
                 1235 
                 1250 
                 TGGAAGACATCCAGAG 
                 50 
                 24191 
                 24206 
                 100 
               
               
                   
               
               
                 797161 
                 1244 
                 1259 
                 TTCCAGGCATGGAAGA 
                 68 
                 24200 
                 24215 
                 101 
               
               
                   
               
               
                 797162 
                 1256 
                 1271 
                 GACCGTTGTTGATTCC 
                 28 
                 N/A 
                 N/A 
                 102 
               
               
                   
               
               
                 797163 
                 1264 
                 1279 
                 CAGGGACAGACCGTTG 
                 22 
                 N/A 
                 N/A 
                 103 
               
               
                   
               
               
                 797164 
                 1272 
                 1287 
                 CGCAGCATCAGGGACA 
                 14 
                 24569 
                 24584 
                 104 
               
               
                   
               
               
                 797165 
                 1289 
                 1304 
                 AGTCATTCTGCTCTGC 
                 9 
                 24586 
                 24601 
                 105 
               
               
                   
               
               
                 797166 
                 1293 
                 1308 
                 ATGAAGTCATTCTGCT 
                 27 
                 24590 
                 24605 
                 106 
               
               
                   
               
               
                 797167 
                 1297 
                 1312 
                 GGGAATGAAGTCATTC 
                 37 
                 24594 
                 24609 
                 107 
               
               
                   
               
               
                 797168 
                 1315 
                 1330 
                 AGTCACTGTGGACAGC 
                 20 
                 24612 
                 24627 
                 108 
               
               
                   
               
               
                 797169 
                 1330 
                 1345 
                 CATTACCCGGGCCCCA 
                 53 
                 24627 
                 24642 
                 109 
               
               
                   
               
               
                 797170 
                 1355 
                 1370 
                 AGGCAGGTTCATCCTG 
                 54 
                 24652 
                 24667 
                 110 
               
               
                   
               
               
                 797171 
                 1362 
                 1377 
                 TCCATAAAGGCAGGTT 
                 48 
                 24659 
                 24674 
                 111 
               
               
                   
               
               
                 797172 
                 1370 
                 1385 
                 CACCATCATCCATAAA 
                 48 
                 24667 
                 24682 
                 112 
               
               
                   
               
               
                 797173 
                 1373 
                 1388 
                 AGCCACCATCATCCAT 
                 31 
                 24670 
                 24685 
                 113 
               
               
                   
               
               
                 797174 
                 1377 
                 1392 
                 TTAAAGCCACCATCAT 
                 39 
                 24674 
                 24689 
                 114 
               
               
                   
               
               
                 797175 
                 1384 
                 1399 
                 CCGCAAGTTAAAGCCA 
                 24 
                 24681 
                 24696 
                 115 
               
               
                   
               
               
                 797176 
                 1391 
                 1406 
                 CGCCAGGCCGCAAGTT 
                 18 
                 24688 
                 24703 
                 116 
               
               
                   
               
               
                 797177 
                 1414 
                 1429 
                 CCTCATGCTGATGGAG 
                 40 
                 24711 
                 24726 
                 117 
               
               
                   
               
               
                 797178 
                 1429 
                 1444 
                 GTCCAGGGTTTCCTTC 
                 48 
                 N/A 
                 N/A 
                 118 
               
               
                   
               
               
                 797179 
                 1439 
                 1454 
                 CCCCAAGTCTGTCCAG 
                 49 
                 25159 
                 25174 
                 119 
               
               
                   
               
               
                 797180 
                 1449 
                 1464 
                 CCATAATCGCCCCCAA 
                 15 
                 25169 
                 25184 
                 120 
               
               
                   
               
               
                 797181 
                 1465 
                 1480 
                 ATTCTTGGTGCAGTCG 
                 18 
                 25185 
                 25200 
                 121 
               
               
                   
               
               
                 797182 
                 1475 
                 1490 
                 CATCACTGCCATTCTT 
                 30 
                 25195 
                 25210 
                 122 
               
               
                   
               
               
                 797183 
                 1482 
                 1497 
                 ACAGGAACATCACTGC 
                 22 
                 25202 
                 25217 
                 123 
               
               
                   
               
               
                 797184 
                 1495 
                 1510 
                 GTAAAGGTTCTCAACA 
                 84 
                 25215 
                 25230 
                 124 
               
               
                   
               
               
                 797185 
                 1502 
                 1517 
                 TTGAAGGGTAAAGGTT 
                 57 
                 25222 
                 25237 
                 125 
               
               
                   
               
               
                 797186 
                 1524 
                 1539 
                 ATACACACCTGCTGTG 
                 87 
                 N/A 
                 N/A 
                 126 
               
               
                   
               
               
                 797187 
                 1533 
                 1548 
                 CAGGAGTGAATACACA 
                 65 
                 25447 
                 25462 
                 127 
               
               
                   
               
               
                 797188 
                 1552 
                 1567 
                 GATCATGCTCTCCTGG 
                 13 
                 25466 
                 25481 
                 128 
               
               
                   
               
               
                 797189 
                 1563 
                 1578 
                 CCACACTCCTTGATCA 
                 36 
                 25477 
                 25492 
                 129 
               
               
                   
               
               
                 797190 
                 1570 
                 1585 
                 GGCACAGCCACACTCC 
                 29 
                 25484 
                 25499 
                 130 
               
               
                   
               
               
                 797191 
                 1573 
                 1588 
                 GTAGGCACAGCCACAC 
                 18 
                 25487 
                 25502 
                 131 
               
               
                   
               
               
                 797192 
                 1578 
                 1593 
                 AAGATGTAGGCACAGC 
                 17 
                 25492 
                 25507 
                 132 
               
               
                   
               
               
                 797193 
                 1586 
                 1601 
                 GCGGATAGAAGATGTA 
                 20 
                 25500 
                 25515 
                 133 
               
               
                   
               
               
                 797194 
                 1613 
                 1628 
                 AGTCACAGTACTCCAC 
                 40 
                 25527 
                 25542 
                 134 
               
               
                   
               
               
                 797195 
                 1623 
                 1638 
                 TGCTTTCTGTAGTCAC 
                 39 
                 25537 
                 25552 
                 135 
               
               
                   
               
               
                 797196 
                 1631 
                 1646 
                 AGGAACTGTGCTTTCT 
                 48 
                 25545 
                 25560 
                 136 
               
               
                   
               
               
                 797197 
                 1644 
                 1659 
                 TAGCAGTACCCCCAGG 
                 51 
                 N/A 
                 N/A 
                 137 
               
               
                   
               
               
                 797198 
                 1651 
                 1666 
                 CTTATAGTAGCAGTAC 
                 42 
                 30597 
                 30612 
                 138 
               
               
                   
               
               
                 797199 
                 1663 
                 1678 
                 GTCAACCTGGAGCTTA 
                 14 
                 30609 
                 30624 
                 139 
               
               
                   
               
               
                 797200 
                 1671 
                 1686 
                 GAGGAGAAGTCAACCT 
                 66 
                 30617 
                 30632 
                 140 
               
               
                   
               
               
                 797201 
                 1684 
                 1699 
                 GCCCAGGTGGTCTGAG 
                 43 
                 30630 
                 30645 
                 141 
               
               
                   
               
               
                 797202 
                 1692 
                 1707 
                 GTGAAACAGCCCAGGT 
                 55 
                 30638 
                 30653 
                 142 
               
               
                   
               
               
                 797203 
                 1700 
                 1715 
                 GGCACTTGGTGAAACA 
                 77 
                 30646 
                 30661 
                 143 
               
               
                   
               
               
                 797204 
                 1707 
                 1722 
                 GGCTTCCGGCACTTGG 
                 22 
                 30653 
                 30668 
                 144 
               
               
                   
               
               
                 797205 
                 1715 
                 1730 
                 CGCTGCATGGCTTCCG 
                 23 
                 N/A 
                 N/A 
                 145 
               
               
                   
               
               
                 797206 
                 1731 
                 1746 
                 AGCTGGTAGCTGGTCA 
                 49 
                 30782 
                 30797 
                 146 
               
               
                   
               
               
                 797207 
                 1739 
                 1754 
                 CAGCAGAGAGCTGGTA 
                 52 
                 30790 
                 30805 
                 147 
               
               
                   
               
               
                 797208 
                 1746 
                 1761 
                 GAGTAACCAGCAGAGA 
                 47 
                 30797 
                 30812 
                 148 
               
               
                   
               
               
                 797218 
                 1875 
                 1890 
                 TTGTAGTTCAGCTCCT 
                 41 
                 31231 
                 31246 
                 149 
               
               
                   
               
               
                 797219 
                 1885 
                 1900 
                 AGAATTGGTTTTGTAG 
                 55 
                 31241 
                 31256 
                 150 
               
               
                   
               
               
                 797220 
                 1895 
                 1910 
                 AGGGAGACTCAGAATT 
                 101 
                 31251 
                 31266 
                 151 
               
               
                   
               
               
                 797221 
                 1919 
                 1934 
                 ACAGGAGGGTGACCAT 
                 41 
                 31748 
                 31763 
                 152 
               
               
                   
               
               
                 797222 
                 1941 
                 1956 
                 CTCCACTGGCTGCCCA 
                 56 
                 31770 
                 31785 
                 153 
               
               
                   
               
               
                 797223 
                 1948 
                 1963 
                 CCACAGGCTCCACTGG 
                 69 
                 31777 
                 31792 
                 154 
               
               
                   
               
               
                 797224 
                 1950 
                 1965 
                 AACCACAGGCTCCACT 
                 64 
                 31779 
                 31794 
                 155 
               
               
                   
               
               
                 797225 
                 1955 
                 1970 
                 AGCCGAACCACAGGCT 
                 139 
                 31784 
                 31799 
                 156 
               
               
                   
               
               
                 797226 
                 1963 
                 1978 
                 CACCGAGGAGCCGAAC 
                 76 
                 31792 
                 31807 
                 157 
               
               
                   
               
               
                 797227 
                 1971 
                 1986 
                 ACAGACAACACCGAGG 
                 24 
                 31800 
                 31815 
                 158 
               
               
                   
               
               
                 797228 
                 1978 
                 1993 
                 CTCCACCACAGACAAC 
                 46 
                 31807 
                 31822 
                 159 
               
               
                   
               
               
                 797229 
                 1988 
                 2003 
                 GCTCAGCCATCTCCAC 
                 63 
                 31817 
                 31832 
                 160 
               
               
                   
               
               
                 797230 
                 1997 
                 2012 
                 CAAAGACGAGCTCAGC 
                 99 
                 31826 
                 31841 
                 161 
               
               
                   
               
               
                 797231 
                 2005 
                 2020 
                 CAGCAGGTCAAAGACG 
                 96 
                 31834 
                 31849 
                 162 
               
               
                   
               
               
                 797232 
                 2017 
                 2032 
                 GAACATGATGACCAGC 
                 22 
                 31846 
                 31861 
                 163 
               
               
                   
               
               
                 797233 
                 2024 
                 2039 
                 GCATGAGGAACATGAT 
                 41 
                 31853 
                 31868 
                 164 
               
               
                   
               
               
                 797234 
                 2037 
                 2052 
                 AACCTTCGGAGCAGCA 
                 18 
                 31866 
                 31881 
                 165 
               
               
                   
               
               
                 797235 
                 2045 
                 2060 
                 GGCTTCGGAACCTTCG 
                 19 
                 31874 
                 31889 
                 166 
               
               
                   
               
               
                 797236 
                 2052 
                 2067 
                 CAGTATCGGCTTCGGA 
                 19 
                 31881 
                 31896 
                 167 
               
               
                   
               
               
                 797237 
                 2060 
                 2075 
                 CTGGAGACCAGTATCG 
                 35 
                 31889 
                 31904 
                 168 
               
               
                   
               
               
                 797238 
                 2104 
                 2119 
                 TGCCAGGGTGGAGGCT 
                 81 
                 31933 
                 31948 
                 169 
               
               
                   
               
               
                 797239 
                 2127 
                 2142 
                 CAGAAGTGGGAAGGAG 
                 63 
                 31956 
                 31971 
                 170 
               
               
                   
               
               
                 797240 
                 2151 
                 2166 
                 AAGGACAGAGACATGG 
                 33 
                 31980 
                 31995 
                 171 
               
               
                   
               
               
                 797241 
                 2187 
                 2202 
                 GTCAAGGCTGGAGAGG 
                 33 
                 32016 
                 32031 
                 172 
               
               
                   
               
               
                 797242 
                 2218 
                 2233 
                 GCCCAGGGTGGCATAG 
                 76 
                 32047 
                 32062 
                 173 
               
               
                   
               
               
                 797243 
                 2259 
                 2274 
                 GAGGAACTGGCCCCTG 
                 58 
                 32088 
                 32103 
                 174 
               
               
                   
               
               
                 797244 
                 2268 
                 2283 
                 GGACAGGTGGAGGAAC 
                 76 
                 32097 
                 32112 
                 175 
               
               
                   
               
               
                 797245 
                 2275 
                 2290 
                 CCCCAGAGGACAGGTG 
                 73 
                 32104 
                 32119 
                 176 
               
               
                   
               
               
                 797246 
                 2304 
                 2319 
                 GAGAAACCTCTCCTTC 
                 64 
                 32133 
                 32148 
                 177 
               
               
                   
               
               
                 797247 
                 2329 
                 2344 
                 ACCAGAGGAGCATCTG 
                 34 
                 32158 
                 32173 
                 178 
               
               
                   
               
               
                 797248 
                 2350 
                 2365 
                 TGCCAGGGCCAGCACC 
                 50 
                 32179 
                 32194 
                 179 
               
               
                   
               
               
                 797249 
                 2358 
                 2373 
                 TTCAATCTTGCCAGGG 
                 28 
                 32187 
                 32202 
                 180 
               
               
                   
               
               
                 797250 
                 2366 
                 2381 
                 GCACATCCTTCAATCT 
                 36 
                 32195 
                 32210 
                 181 
               
               
                   
               
               
                 797251 
                 2377 
                 2392 
                 GAGGAAGCCCTGCACA 
                 51 
                 32206 
                 32221 
                 182 
               
               
                   
               
               
                 797252 
                 2394 
                 2409 
                 AGTTTGGGCGGCTCTG 
                 54 
                 32223 
                 32238 
                 183 
               
               
                   
               
               
                 797253 
                 2402 
                 2417 
                 TCAACGGCAGTTTGGG 
                 22 
                 32231 
                 32246 
                 184 
               
               
                   
               
               
                 797254 
                 2409 
                 2424 
                 CCACACATCAACGGCA 
                 23 
                 32238 
                 32253 
                 185 
               
               
                   
               
               
                 797255 
                 2427 
                 2442 
                 ACCCATCTTGCTTCCC 
                 22 
                 32256 
                 32271 
                 186 
               
               
                   
               
               
                 797256 
                 2446 
                 2461 
                 AGCAACTTCCTGAGCC 
                 36 
                 32275 
                 32290 
                 187 
               
               
                   
               
               
                 797257 
                 2461 
                 2476 
                 AGCTACTGTTCTTGGA 
                 23 
                 32290 
                 32305 
                 188 
               
               
                   
               
               
                 797258 
                 2481 
                 2496 
                 CACTTCTGGGCAGCTT 
                 14 
                 32310 
                 32325 
                 189 
               
               
                   
               
               
                 797259 
                 2488 
                 2503 
                 GCCAAGGCACTTCTGG 
                 41 
                 32317 
                 32332 
                 190 
               
               
                   
               
               
                 797260 
                 2501 
                 2516 
                 GTACAGGGCTGGAGCC 
                 65 
                 32330 
                 32345 
                 191 
               
               
                   
               
               
                 797261 
                 2522 
                 2537 
                 GTTCAGAGGCAGTACC 
                 24 
                 32351 
                 32366 
                 192 
               
               
                   
               
               
                 797262 
                 2529 
                 2544 
                 CCAGAGTGTTCAGAGG 
                 17 
                 32358 
                 32373 
                 193 
               
               
                   
               
               
                 797263 
                 2550 
                 2565 
                 AGCCGCAGTTGGGTGG 
                 28 
                 32379 
                 32394 
                 194 
               
               
                   
               
               
                 797264 
                 2558 
                 2573 
                 AGAGACTTAGCCGCAG 
                 12 
                 32387 
                 32402 
                 195 
               
               
                   
               
               
                 797265 
                 2565 
                 2580 
                 GGGAAAAAGAGACTTA 
                 21 
                 32394 
                 32409 
                 196 
               
               
                   
               
               
                 797266 
                 2576 
                 2591 
                 GGCTGATCCAAGGGAA 
                 14 
                 32405 
                 32420 
                 197 
               
               
                   
               
               
                 797267 
                 2590 
                 2605 
                 TCCAAGTTTCGCTTGG 
                 85 
                 32419 
                 32434 
                 198 
               
               
                   
               
               
                 797268 
                 2598 
                 2613 
                 GTCAAAGCTCCAAGTT 
                 26 
                 32427 
                 32442 
                 199 
               
               
                   
               
               
                 797269 
                 2611 
                 2626 
                 AGGAAAGTTCCTTGTC 
                 21 
                 32440 
                 32455 
                 200 
               
               
                   
               
               
                 797270 
                 2626 
                 2641 
                 TATCAGCGGTTTCTTA 
                 52 
                 32455 
                 32470 
                 201 
               
               
                   
               
               
                 797271 
                 2675 
                 2690 
                 GGAAACCCGTGCATGC 
                 27 
                 32504 
                 32519 
                 202 
               
               
                   
               
               
                 797272 
                 2684 
                 2699 
                 CGCTGGGCAGGAAACC 
                 25 
                 32513 
                 32528 
                 203 
               
               
                   
               
               
                 797273 
                 2694 
                 2709 
                 CTTAAGCCGTCGCTGG 
                 31 
                 32523 
                 32538 
                 204 
               
               
                   
               
               
                 797274 
                 2716 
                 2731 
                 GGCCAGGCCAGTCGGG 
                 64 
                 32545 
                 32560 
                 205 
               
               
                   
               
               
                 797275 
                 2725 
                 2740 
                 GAGCAGTGTGGCCAGG 
                 18 
                 32554 
                 32569 
                 206 
               
               
                   
               
               
                 797276 
                 2732 
                 2747 
                 TACTGGAGAGCAGTGT 
                 24 
                 32561 
                 32576 
                 207 
               
               
                   
               
               
                 797277 
                 2744 
                 2759 
                 AGACATCTGTGCTACT 
                 10 
                 32573 
                 32588 
                 208 
               
               
                   
               
               
                 797278 
                 2757 
                 2772 
                 CAAGAGGAGGAGCAGA 
                 46 
                 32586 
                 32601 
                 209 
               
               
                   
               
               
                 797279 
                 2765 
                 2780 
                 CCCAAGTTCAAGAGGA 
                 68 
                 32594 
                 32609 
                 210 
               
               
                   
               
               
                 797280 
                 2803 
                 2818 
                 CCTAAGTAACAAAGGG 
                 44 
                 32632 
                 32647 
                 211 
               
               
                   
               
               
                 797281 
                 2811 
                 2826 
                 GGGAATTGCCTAAGTA 
                 44 
                 32640 
                 32655 
                 212 
               
               
                   
               
               
                 797282 
                 2857 
                 2872 
                 ACTTACCCGGGTCTGC 
                 77 
                 32686 
                 32701 
                 213 
               
               
                   
               
               
                 797283 
                 2864 
                 2879 
                 TGCCTTTACTTACCCG 
                 34 
                 32693 
                 32708 
                 214 
               
               
                   
               
               
                 797284 
                 2883 
                 2898 
                 GCTAGAGGAGCCCTGG 
                 60 
                 32712 
                 32727 
                 215 
               
               
                   
               
               
                 797285 
                 2890 
                 2905 
                 GTATGAGGCTAGAGGA 
                 70 
                 32719 
                 32734 
                 216 
               
               
                   
               
               
                 797286 
                 2897 
                 2912 
                 GGCACGGGTATGAGGC 
                 71 
                 32726 
                 32741 
                 217 
               
               
                   
               
               
                 797287 
                 2914 
                 2929 
                 GGGCATGGCTCTGTGA 
                 34 
                 32743 
                 32758 
                 218 
               
               
                   
               
               
                 797288 
                 2937 
                 2952 
                 AAAGACACAGGGCAGA 
                 27 
                 32766 
                 32781 
                 219 
               
               
                   
               
               
                 797289 
                 2944 
                 2959 
                 AGGTATGAAAGACACA 
                 17 
                 32773 
                 32788 
                 220 
               
               
                   
               
               
                 797290 
                 2952 
                 2967 
                 ACATGTAGAGGTATGA 
                 17 
                 32781 
                 32796 
                 221 
               
               
                   
               
               
                 797291 
                 2962 
                 2977 
                 TCTCAAGCAGACATGT 
                 20 
                 32791 
                 32806 
                 222 
               
               
                   
               
               
                 797292 
                 2969 
                 2984 
                 GGAAATATCTCAAGCA 
                 15 
                 32798 
                 32813 
                 223 
               
               
                   
               
               
                 797293 
                 2983 
                 2998 
                 AACTTTCAGGCTGAGG 
                 15 
                 32812 
                 32827 
                 224 
               
               
                   
               
               
                 797294 
                 3013 
                 3028 
                 CATAGGAGTTCTCTGG 
                 10 
                 32842 
                 32857 
                 225 
               
               
                   
               
               
                 797295 
                 3020 
                 3035 
                 AGGGATGCATAGGAGT 
                 19 
                 32849 
                 32864 
                 226 
               
               
                   
               
               
                 797296 
                 3033 
                 3048 
                 GAGCAGGGTTCTAAGG 
                 60 
                 32862 
                 32877 
                 227 
               
               
                   
               
               
                 797297 
                 3046 
                 3061 
                 AGTAATGGTGTCTGAG 
                 12 
                 32875 
                 32890 
                 228 
               
               
                   
               
               
                 797298 
                 3053 
                 3068 
                 TCACAAAAGTAATGGT 
                 26 
                 32882 
                 32897 
                 229 
               
               
                   
               
               
                 797299 
                 3072 
                 3087 
                 CAAGATGTGGCAGAAG 
                 58 
                 32901 
                 32916 
                 230 
               
               
                   
               
               
                 797300 
                 3079 
                 3094 
                 GGGAAGACAAGATGTG 
                 22 
                 32908 
                 32923 
                 231 
               
               
                   
               
               
                 797301 
                 3093 
                 3108 
                 AGTGATCAATTTTGGG 
                 22 
                 32922 
                 32937 
                 232 
               
               
                   
               
               
                 797302 
                 3103 
                 3118 
                 GAGAAGGCGGAGTGAT 
                 61 
                 32932 
                 32947 
                 233 
               
               
                   
               
               
                 797303 
                 3118 
                 3133 
                 GCTACGGGAGCCCAGG 
                 27 
                 32947 
                 32962 
                 234 
               
               
                   
               
               
                 797304 
                 3127 
                 3142 
                 TTATAGTGTGCTACGG 
                 13 
                 32956 
                 32971 
                 235 
               
               
                   
               
               
                 797305 
                 3134 
                 3149 
                 GCAGATGTTATAGTGT 
                 21 
                 32963 
                 32978 
                 236 
               
               
                   
               
               
                 797306 
                 3144 
                 3159 
                 CAACACTCCAGCAGAT 
                 53 
                 32973 
                 32988 
                 237 
               
               
                   
               
               
                 797307 
                 3151 
                 3166 
                 GCAACAGCAACACTCC 
                 6 
                 32980 
                 32995 
                 238 
               
               
                   
               
               
                 797308 
                 3160 
                 3175 
                 AAGTATGGTGCAACAG 
                 9 
                 32989 
                 33004 
                 239 
               
               
                   
               
               
                 797309 
                 3167 
                 3182 
                 TACAAGAAAGTATGGT 
                 11 
                 32996 
                 33011 
                 240 
               
               
                   
               
               
                 797310 
                 3180 
                 3195 
                 GGAGACACAAATGTAC 
                 37 
                 33009 
                 33024 
                 241 
               
               
                   
               
               
                 797311 
                 3199 
                 3214 
                 TTACAGTCTAGTTGGG 
                 20 
                 33028 
                 33043 
                 242 
               
               
                   
               
               
                 797312 
                 3209 
                 3224 
                 CGCAAGGCACTTACAG 
                 13 
                 33038 
                 33053 
                 243 
               
               
                   
               
               
                 797313 
                 3227 
                 3242 
                 CAAGATTCAGTCCCTG 
                 15 
                 33056 
                 33071 
                 244 
               
               
                   
               
               
                 797314 
                 3234 
                 3249 
                 AAACGGGCAAGATTCA 
                 21 
                 33063 
                 33078 
                 245 
               
               
                   
               
               
                 797315 
                 3241 
                 3256 
                 CATACATAAACGGGCA 
                 19 
                 33070 
                 33085 
                 246 
               
               
                   
               
               
                 797316 
                 3248 
                 3263 
                 CATGGAGCATACATAA 
                 52 
                 33077 
                 33092 
                 247 
               
               
                   
               
               
                 797317 
                 3255 
                 3270 
                 GGCTAGACATGGAGCA 
                 39 
                 33084 
                 33099 
                 248 
               
               
                   
               
               
                 797318 
                 3263 
                 3278 
                 GGATGATGGGCTAGAC 
                 18 
                 33092 
                 33107 
                 249 
               
               
                   
               
               
                 797319 
                 3271 
                 3286 
                 TCCAAGCAGGATGATG 
                 82 
                 33100 
                 33115 
                 250 
               
               
                   
               
               
                 797320 
                 3282 
                 3297 
                 TGCCTACTTGCTCCAA 
                 51 
                 33111 
                 33126 
                 251 
               
               
                   
               
               
                 797321 
                 3291 
                 3306 
                 TTGAGCTCCTGCCTAC 
                 21 
                 33120 
                 33135 
                 252 
               
               
                   
               
               
                 797322 
                 3293 
                 3308 
                 TATTGAGCTCCTGCCT 
                 52 
                 33122 
                 33137 
                 253 
               
               
                   
               
               
                 797323 
                 3294 
                 3309 
                 TTATTGAGCTCCTGCC 
                 35 
                 33123 
                 33138 
                 254 
               
               
                   
               
               
                 797324 
                 N/A 
                 N/A 
                 AATCAGTTTTCTGAGG 
                 80 
                 2644 
                 2659 
                 255 
               
               
                   
               
               
                 797325 
                 N/A 
                 N/A 
                 AAGGATAAATCAGTTT 
                 98 
                 2651 
                 2666 
                 256 
               
               
                   
               
               
                 797326 
                 N/A 
                 N/A 
                 AGAAACTGACCCTTCC 
                 75 
                 2668 
                 2683 
                 257 
               
               
                   
               
               
                 797327 
                 N/A 
                 N/A 
                 CCTAATGAAGAAACTG 
                 78 
                 2676 
                 2691 
                 258 
               
               
                   
               
               
                 797328 
                 N/A 
                 N/A 
                 CTTCATTGTCCTAATG 
                 103 
                 2685 
                 2700 
                 259 
               
               
                   
               
               
                 797329 
                 N/A 
                 N/A 
                 AGCTGGATTTTTCTTC 
                 68 
                 2697 
                 2712 
                 260 
               
               
                   
               
               
                 797330 
                 N/A 
                 N/A 
                 GAAGGGACAGCTGGAT 
                 64 
                 2705 
                 2720 
                 261 
               
               
                   
               
               
                 797331 
                 N/A 
                 N/A 
                 CATGATACCTCCCCTT 
                 47 
                 2722 
                 2737 
                 262 
               
               
                   
               
               
                 797332 
                 N/A 
                 N/A 
                 TGATACTGCTCATGAT 
                 73 
                 2732 
                 2747 
                 263 
               
               
                   
               
               
                 797333 
                 N/A 
                 N/A 
                 TCCTAGCACCTCCCTT 
                 57 
                 4867 
                 4882 
                 264 
               
               
                   
               
               
                 797334 
                 N/A 
                 N/A 
                 ACCTTTCGAGTTTTGT 
                 26 
                 4882 
                 4897 
                 265 
               
               
                   
               
               
                 797335 
                 N/A 
                 N/A 
                 TGATAGGGCCACCTTT 
                 47 
                 4892 
                 4907 
                 266 
               
               
                   
               
               
                 797336 
                 N/A 
                 N/A 
                 CTAGAACGGCCTCTCC 
                 48 
                 4918 
                 4933 
                 267 
               
               
                   
               
               
                 797337 
                 N/A 
                 N/A 
                 CCGGAGCTGGGCTTCC 
                 69 
                 4934 
                 4949 
                 268 
               
               
                   
               
               
                 797338 
                 N/A 
                 N/A 
                 CAAAAGTGCCGGAGCT 
                 36 
                 4942 
                 4957 
                 269 
               
               
                   
               
               
                 797339 
                 N/A 
                 N/A 
                 CAGCAGACCTGCGGGA 
                 33 
                 4966 
                 4981 
                 270 
               
               
                   
               
               
                 797340 
                 N/A 
                 N/A 
                 CTGGAGCCAGCAGACC 
                 16 
                 4973 
                 4988 
                 271 
               
               
                   
               
               
                 797341 
                 N/A 
                 N/A 
                 CCACATTCTCCCACTC 
                 50 
                 5011 
                 5026 
                 272 
               
               
                   
               
               
                 797342 
                 N/A 
                 N/A 
                 TCCCACCCTGCGCCCA 
                 82 
                 5024 
                 5039 
                 273 
               
               
                   
               
               
                 797343 
                 N/A 
                 N/A 
                 GGCCATGCCCATGTCC 
                 65 
                 5037 
                 5052 
                 274 
               
               
                   
               
               
                 797344 
                 N/A 
                 N/A 
                 CCCGAGTGAGGCTGCC 
                 22 
                 5056 
                 5071 
                 275 
               
               
                   
               
               
                 797345 
                 N/A 
                 N/A 
                 GGCTGAGCTCTGGGCC 
                 72 
                 5101 
                 5116 
                 276 
               
               
                   
               
               
                 797346 
                 N/A 
                 N/A 
                 GGTCAGGGTCAAGGCT 
                 50 
                 5113 
                 5128 
                 277 
               
               
                   
               
               
                 797347 
                 N/A 
                 N/A 
                 CCCCGGAGTGGATTGG 
                 71 
                 5139 
                 5154 
                 278 
               
               
                   
               
               
                 797348 
                 N/A 
                 N/A 
                 GGCCTTTCGGCTTGAG 
                 72 
                 15860 
                 15875 
                 279 
               
               
                   
               
               
                 797349 
                 N/A 
                 N/A 
                 GGCGAGTGTCGGTGGC 
                 43 
                 15873 
                 15888 
                 280 
               
               
                   
               
               
                 797350 
                 N/A 
                 N/A 
                 GGTCATCCCGCCCTGA 
                 40 
                 15897 
                 15912 
                 281 
               
               
                   
               
               
                 797351 
                 N/A 
                 N/A 
                 CGGTACCCAGGTCATC 
                 51 
                 15906 
                 15921 
                 282 
               
               
                   
               
               
                 797352 
                 N/A 
                 N/A 
                 CGTGACCGCGGTACCC 
                 57 
                 15914 
                 15929 
                 283 
               
               
                   
               
               
                 797353 
                 N/A 
                 N/A 
                 GGTGAGGGCCTCGGCC 
                 88 
                 15934 
                 15949 
                 284 
               
               
                   
               
               
                 797354 
                 N/A 
                 N/A 
                 CGGAACTTGTCTGCCC 
                 45 
                 15968 
                 15983 
                 285 
               
               
                   
               
               
                 797355 
                 N/A 
                 N/A 
                 GGGAACCCGGAACTTG 
                 85 
                 15975 
                 15990 
                 286 
               
               
                   
               
               
                 797356 
                 N/A 
                 N/A 
                 CCTAGAAGGGAACCCG 
                 38 
                 15982 
                 15997 
                 287 
               
               
                   
               
               
                 797357 
                 N/A 
                 N/A 
                 GCCCGGACCTAGAAGG 
                 82 
                 15989 
                 16004 
                 288 
               
               
                   
               
               
                 797358 
                 N/A 
                 N/A 
                 AGAGAGGCAGGAGGCG 
                 104 
                 16034 
                 16049 
                 289 
               
               
                   
               
               
                 797359 
                 N/A 
                 N/A 
                 TTGAAGGAGAGAGGCA 
                 52 
                 16041 
                 16056 
                 290 
               
               
                   
               
               
                 797360 
                 N/A 
                 N/A 
                 GTGGACTGTTTATTGA 
                 67 
                 16053 
                 16068 
                 291 
               
               
                   
               
               
                 797361 
                 N/A 
                 N/A 
                 GGACACTGTGGACTGT 
                 51 
                 16060 
                 16075 
                 292 
               
               
                   
               
               
                 797362 
                 N/A 
                 N/A 
                 GCCCAGCCGGGACACT 
                 71 
                 16069 
                 16084 
                 293 
               
               
                   
               
               
                 797363 
                 N/A 
                 N/A 
                 CCACGGCGAGCCCAGC 
                 69 
                 16078 
                 16093 
                 294 
               
               
                   
               
               
                 797364 
                 N/A 
                 N/A 
                 GCGGAGGCCACGGCGA 
                 83 
                 16085 
                 16100 
                 295 
               
               
                   
               
               
                 797365 
                 N/A 
                 N/A 
                 CGGGAAGGCGGAGGCC 
                 78 
                 16092 
                 16107 
                 296 
               
               
                   
               
               
                 797366 
                 N/A 
                 N/A 
                 AAACAGGTGTGTCCGC 
                 77 
                 16108 
                 16123 
                 297 
               
               
                   
               
               
                 797367 
                 N/A 
                 N/A 
                 GTGAAGGGTAAACAGG 
                 77 
                 16117 
                 16132 
                 298 
               
               
                   
               
               
                 797368 
                 N/A 
                 N/A 
                 GGCCGTCCGGCGGTGA 
                 55 
                 16129 
                 16144 
                 299 
               
               
                   
               
               
                 797369 
                 N/A 
                 N/A 
                 GGAGAAGCCTGGGCGG 
                 83 
                 16170 
                 16185 
                 300 
               
               
                   
               
               
                 797370 
                 N/A 
                 N/A 
                 GTCCATCCCGGAGAAG 
                 63 
                 16179 
                 16194 
                 301 
               
               
                   
               
               
                 797371 
                 N/A 
                 N/A 
                 CGGGAGGCCGGTCCAT 
                 52 
                 16189 
                 16204 
                 302 
               
               
                   
               
               
                 797372 
                 N/A 
                 N/A 
                 GGTCAGGGTCCTCATC 
                 79 
                 16212 
                 16227 
                 303 
               
               
                   
               
               
                 797373 
                 N/A 
                 N/A 
                 AGCGAGTGTCTGGCCC 
                 94 
                 16234 
                 16249 
                 304 
               
               
                   
               
               
                 797374 
                 N/A 
                 N/A 
                 GAAGAGGGTCAGGCCA 
                 52 
                 16260 
                 16275 
                 305 
               
               
                   
               
               
                 797375 
                 N/A 
                 N/A 
                 GAGTAATTCCTGGTTG 
                 103 
                 N/A 
                 N/A 
                 306 
               
               
                   
               
               
                 797376 
                 N/A 
                 N/A 
                 TGTCTTTAAAACGGAC 
                 112 
                 3040 
                 3055 
                 307 
               
               
                   
               
               
                 797377 
                 N/A 
                 N/A 
                 GAGGAGATAGGCCTGC 
                 42 
                 3098 
                 3113 
                 308 
               
               
                   
               
               
                 797378 
                 N/A 
                 N/A 
                 AAAAAGGGCTGGAGGA 
                 113 
                 3291 
                 3306 
                 309 
               
               
                   
               
               
                 797379 
                 N/A 
                 N/A 
                 AATCAGACCCAAAAAG 
                 134 
                 3301 
                 3316 
                 310 
               
               
                   
               
               
                 797380 
                 N/A 
                 N/A 
                 GAGGAGGGTCAGAGAA 
                 69 
                 3315 
                 3330 
                 311 
               
               
                   
               
               
                 797381 
                 N/A 
                 N/A 
                 TTGCAGGAATGTGGGC 
                 80 
                 3593 
                 3608 
                 312 
               
               
                   
               
               
                 797382 
                 N/A 
                 N/A 
                 GGCCAGGAATGTGTAA 
                 103 
                 3632 
                 3647 
                 313 
               
               
                   
               
               
                 797383 
                 N/A 
                 N/A 
                 GGACATTCTGTTCTTT 
                 97 
                 3667 
                 3682 
                 314 
               
               
                   
               
               
                 797384 
                 N/A 
                 N/A 
                 GACAATAGAGAGGGAC 
                 93 
                 4090 
                 4105 
                 315 
               
               
                   
               
               
                 797385 
                 N/A 
                 N/A 
                 GCAGAAAGAGGGAGAC 
                 85 
                 4103 
                 4118 
                 316 
               
               
                   
               
               
                 797386 
                 346 
                 361 
                 GTTCCCCTTCATGAGC 
                 26 
                 5154 
                 5169 
                 317 
               
               
                   
                   
                   
                   
                   
                 5274 
                 5289 
                   
               
               
                   
               
               
                 797387 
                 349 
                 364 
                 CTTGTTCCCCTTCATG 
                 15 
                 5157 
                 5172 
                 318 
               
               
                   
                   
                   
                   
                   
                 5277 
                 5292 
                   
               
               
                   
               
               
                 797388 
                 N/A 
                 N/A 
                 AGTAAGCTGGAGGCTC 
                 69 
                 5784 
                 5799 
                 319 
               
               
                   
               
               
                 797389 
                 N/A 
                 N/A 
                 TGCAAGCACTCCCTCC 
                 40 
                 5932 
                 5947 
                 320 
               
               
                   
               
               
                 797390 
                 N/A 
                 N/A 
                 GAAAAGGGATGCAGCT 
                 81 
                 5967 
                 5982 
                 321 
               
               
                   
               
               
                 797391 
                 N/A 
                 N/A 
                 AGCATTTTAGCCTGGG 
                 20 
                 6265 
                 6280 
                 322 
               
               
                   
               
               
                 797392 
                 N/A 
                 N/A 
                 TATACAAAAGCACTCA 
                 62 
                 6422 
                 6437 
                 323 
               
               
                   
               
               
                 797393 
                 N/A 
                 N/A 
                 GAATTATTCATGAATC 
                 42 
                 6523 
                 6538 
                 324 
               
               
                   
               
               
                 797394 
                 N/A 
                 N/A 
                 TGGAATATACGAAGGG 
                 24 
                 6782 
                 6797 
                 325 
               
               
                   
               
               
                 797395 
                 N/A 
                 N/A 
                 CATATTTTCAACCACA 
                 25 
                 7349 
                 7364 
                 326 
               
               
                   
               
               
                 797396 
                 N/A 
                 N/A 
                 TAATACTGCCCACCTC 
                 76 
                 7746 
                 7761 
                 327 
               
               
                   
               
               
                 797397 
                 N/A 
                 N/A 
                 TTGATTTAGATTCATT 
                 61 
                 8239 
                 8254 
                 328 
               
               
                   
               
               
                 797398 
                 N/A 
                 N/A 
                 ACTTAAAGTGTAATGG 
                 89 
                 8562 
                 8577 
                 329 
               
               
                   
               
               
                 797399 
                 N/A 
                 N/A 
                 TGCCTGATCCCTACTT 
                 59 
                 8574 
                 8589 
                 330 
               
               
                   
               
               
                 797400 
                 N/A 
                 N/A 
                 GAAAAATATGTCTGTG 
                 53 
                 9080 
                 9095 
                 331 
               
               
                   
               
               
                 797401 
                 N/A 
                 N/A 
                 AGCCGTGGGAGCCGCC 
                 31 
                 9458 
                 9473 
                 332 
               
               
                   
               
               
                 797402 
                 N/A 
                 N/A 
                 GTCCAGGACGGAGCAG 
                 32 
                 9587 
                 9602 
                 333 
               
               
                   
               
               
                 797403 
                 N/A 
                 N/A 
                 AAGACATCCGATCTTG 
                 92 
                 10021 
                 10036 
                 334 
               
               
                   
               
               
                 797404 
                 N/A 
                 N/A 
                 CACTAAACAGAAAGCA 
                 38 
                 10042 
                 10057 
                 335 
               
               
                   
               
               
                 797405 
                 N/A 
                 N/A 
                 GCATAAGATAAGACGG 
                 15 
                 10454 
                 10469 
                 336 
               
               
                   
               
               
                 797406 
                 N/A 
                 N/A 
                 CACAAACCTGTGACAA 
                 48 
                 10544 
                 10559 
                 337 
               
               
                   
               
               
                 797407 
                 N/A 
                 N/A 
                 CTCCTGCCACCCTACG 
                 66 
                 10638 
                 10653 
                 338 
               
               
                   
                   
                   
                   
                   
                 10661 
                 10676 
                   
               
               
                   
                   
                   
                   
                   
                 10684 
                 10699 
                   
               
               
                   
                   
                   
                   
                   
                 10665 
                 10680 
                   
               
               
                   
               
               
                 797408 
                 N/A 
                 N/A 
                 CTCCCTCCTGCCACCC 
                 50 
                 10642 
                 10657 
                 339 
               
               
                   
                   
                   
                   
                   
                 10688 
                 10703 
                   
               
               
                   
                   
                   
                   
                   
                 10711 
                 10726 
                   
               
               
                   
               
               
                 797409 
                 N/A 
                 N/A 
                 CACCTCCCTCCTGCCA 
                 43 
                 10645 
                 10660 
                 340 
               
               
                   
                   
                   
                   
                   
                 10668 
                 10683 
                   
               
               
                   
                   
                   
                   
                   
                 10691 
                 10706 
                   
               
               
                   
               
               
                 797410 
                 N/A 
                 N/A 
                 ACGCACCTCCCTCCTG 
                 30 
                 10648 
                 10663 
                 341 
               
               
                   
                   
                   
                   
                   
                 10671 
                 10686 
                   
               
               
                   
               
               
                 797411 
                 N/A 
                 N/A 
                 CCTACGCACCTCCCTC 
                 54 
                 10651 
                 10666 
                 342 
               
               
                   
                   
                   
                   
                   
                 10674 
                 10689 
                   
               
               
                   
               
               
                 797412 
                 N/A 
                 N/A 
                 CACCCTACGCACCTCC 
                 59 
                 10654 
                 10669 
                 343 
               
               
                   
                   
                   
                   
                   
                 10677 
                 10692 
                   
               
               
                   
               
               
                 797413 
                 N/A 
                 N/A 
                 TGCCACCCTACGCACC 
                 29 
                 10657 
                 10672 
                 344 
               
               
                   
                   
                   
                   
                   
                 10680 
                 10695 
                   
               
               
                   
               
               
                 797414 
                 N/A 
                 N/A 
                 GAAGAATCCAGATCCC 
                 56 
                 10944 
                 10959 
                 345 
               
               
                   
               
               
                 797415 
                 N/A 
                 N/A 
                 AGGAAGAATCCAGATC 
                 118 
                 10946 
                 10961 
                 346 
               
               
                   
               
               
                 797416 
                 N/A 
                 N/A 
                 GCGAATTTGCCTTTCT 
                 40 
                 10973 
                 10988 
                 347 
               
               
                   
               
               
                 797417 
                 N/A 
                 N/A 
                 TGAGAAAATACTCAGT 
                 31 
                 11051 
                 11066 
                 348 
               
               
                   
               
               
                 797418 
                 N/A 
                 N/A 
                 TCTGAGATGTAGGGCC 
                 41 
                 11208 
                 11223 
                 349 
               
               
                   
               
               
                 797419 
                 N/A 
                 N/A 
                 CTCCAACCACCACACT 
                 73 
                 11307 
                 11322 
                 350 
               
               
                   
               
               
                 797420 
                 N/A 
                 N/A 
                 TGGCACAGCTAGCAAA 
                 65 
                 11337 
                 11352 
                 351 
               
               
                   
               
               
                 797421 
                 N/A 
                 N/A 
                 CTTTAGGCTAAAACTT 
                 101 
                 11469 
                 11484 
                 352 
               
               
                   
               
               
                 797422 
                 N/A 
                 N/A 
                 CACTATGCATGAAGAA 
                 40 
                 11521 
                 11536 
                 353 
               
               
                   
               
               
                 797423 
                 N/A 
                 N/A 
                 GACAAGTGGGCTGCCT 
                 27 
                 11611 
                 11626 
                 354 
               
               
                   
               
               
                 797424 
                 N/A 
                 N/A 
                 TTAATTGTTAAAAGAA 
                 72 
                 11860 
                 11875 
                 355 
               
               
                   
                   
                   
                   
                   
                 12660 
                 12675 
                   
               
               
                   
               
               
                 797425 
                 N/A 
                 N/A 
                 ATTTAATTGTTAAAAG 
                 103 
                 11862 
                 11877 
                 356 
               
               
                   
                   
                   
                   
                   
                 12662 
                 12677 
                   
               
               
                   
               
               
                 797426 
                 N/A 
                 N/A 
                 TATTTAATTGTTAAAA 
                 113 
                 11863 
                 11878 
                 357 
               
               
                   
                   
                   
                   
                   
                 12663 
                 12678 
                   
               
               
                   
               
               
                 797427 
                 N/A 
                 N/A 
                 CATTATTTAATTGTTA 
                 127 
                 11866 
                 11881 
                 358 
               
               
                   
                   
                   
                   
                   
                 12666 
                 12681 
                   
               
               
                   
               
               
                 797428 
                 N/A 
                 N/A 
                 AAAGAATGGCAAGCAT 
                 25 
                 11937 
                 11952 
                 359 
               
               
                   
               
               
                 797429 
                 N/A 
                 N/A 
                 GGTTGAAGGTGTGTTT 
                 36 
                 11988 
                 12003 
                 360 
               
               
                   
               
               
                 797430 
                 N/A 
                 N/A 
                 TGTCAAACCTGAGTGG 
                 76 
                 12309 
                 12324 
                 361 
               
               
                   
               
               
                 797431 
                 N/A 
                 N/A 
                 CAACATCTCGACTGTC 
                 25 
                 12321 
                 12336 
                 362 
               
               
                   
               
               
                 797432 
                 N/A 
                 N/A 
                 GATAGAGATAGCATTC 
                 71 
                 12401 
                 12416 
                 363 
               
               
                   
               
               
                 797433 
                 N/A 
                 N/A 
                 ACAGACAAAACCAGTT 
                 85 
                 12417 
                 12432 
                 364 
               
               
                   
               
               
                 797434 
                 N/A 
                 N/A 
                 TGGCAATCATAGCTAG 
                 38 
                 12731 
                 12746 
                 365 
               
               
                   
               
               
                 797435 
                 N/A 
                 N/A 
                 CGACGAAACCTTGTAT 
                 53 
                 12931 
                 12946 
                 366 
               
               
                   
               
               
                 797436 
                 N/A 
                 N/A 
                 AAGAATGGTAATCTGC 
                 57 
                 13042 
                 13057 
                 367 
               
               
                   
               
               
                 797437 
                 N/A 
                 N/A 
                 GCCAAAAAGCCTGAAG 
                 28 
                 13125 
                 13140 
                 368 
               
               
                   
               
               
                 797438 
                 N/A 
                 N/A 
                 TCATAGCCATTTTATT 
                 83 
                 13148 
                 13163 
                 369 
               
               
                   
               
               
                 797439 
                 N/A 
                 N/A 
                 AAAGATTTGTACATGA 
                 23 
                 13168 
                 13183 
                 370 
               
               
                   
                   
                   
                   
                   
                 13483 
                 13498 
                   
               
               
                   
               
               
                 797440 
                 N/A 
                 N/A 
                 ATATTAAGAAGGAATG 
                 71 
                 13566 
                 13581 
                 371 
               
               
                   
               
               
                 797441 
                 N/A 
                 N/A 
                 TACGATCATTTTGGAA 
                 42 
                 13770 
                 13785 
                 372 
               
               
                   
               
               
                 797442 
                 N/A 
                 N/A 
                 GAACAGACCTACATTT 
                 74 
                 13832 
                 13847 
                 373 
               
               
                   
                   
                   
                   
                   
                 14129 
                 14144 
                   
               
               
                   
               
               
                 797443 
                 N/A 
                 N/A 
                 CAGACCTACATTTTTT 
                 48 
                 14126 
                 14141 
                 374 
               
               
                   
               
               
                 797444 
                 N/A 
                 N/A 
                 ACCGTATGTAGTAGGC 
                 10 
                 14152 
                 14167 
                 375 
               
               
                   
               
               
                 797445 
                 N/A 
                 N/A 
                 AACAAATAATCCCTAG 
                 88 
                 14172 
                 14187 
                 376 
               
               
                   
               
               
                 797446 
                 N/A 
                 N/A 
                 TCTAGAAGATGGAAGA 
                 56 
                 14238 
                 14253 
                 377 
               
               
                   
               
               
                 797447 
                 N/A 
                 N/A 
                 GAACCATAAACACTCT 
                 19 
                 14251 
                 14266 
                 378 
               
               
                   
               
               
                 797448 
                 N/A 
                 N/A 
                 CTATACAGGCAAAAAT 
                 80 
                 14304 
                 14319 
                 379 
               
               
                   
               
               
                 797449 
                 N/A 
                 N/A 
                 CAAAAGTGTGCCACCA 
                 31 
                 14828 
                 14843 
                 380 
               
               
                   
               
               
                 797450 
                 N/A 
                 N/A 
                 ATGGATTCAACACAAT 
                 26 
                 14993 
                 15008 
                 381 
               
               
                   
               
               
                 797451 
                 N/A 
                 N/A 
                 AAATTAATTGCATTTC 
                 103 
                 15184 
                 15199 
                 382 
               
               
                   
                   
                   
                   
                   
                 15212 
                 15227 
                   
               
               
                   
               
               
                 797452 
                 N/A 
                 N/A 
                 TTGCATTTCCGTCTCA 
                 23 
                 15205 
                 15220 
                 383 
               
               
                   
               
               
                 797453 
                 N/A 
                 N/A 
                 AAAAATTAATTGCATT 
                 86 
                 15214 
                 15229 
                 384 
               
               
                   
                   
                   
                   
                   
                 15530 
                 15545 
                   
               
               
                   
               
               
                 797454 
                 N/A 
                 N/A 
                 AAAAAAATTAATTGCA 
                 106 
                 15216 
                 15231 
                 385 
               
               
                   
                   
                   
                   
                   
                 15532 
                 15547 
                   
               
               
                   
               
               
                 797455 
                 N/A 
                 N/A 
                 AAAAAAAATTAATTGC 
                 67 
                 15217 
                 15232 
                 386 
               
               
                   
                   
                   
                   
                   
                 15533 
                 15548 
                   
               
               
                   
               
               
                 797456 
                 N/A 
                 N/A 
                 TAACAAACTGAACAAG 
                 73 
                 15574 
                 15589 
                 387 
               
               
                   
               
               
                 797457 
                 N/A 
                 N/A 
                 TGTTTCGGGTGCGGCC 
                 62 
                 15731 
                 15746 
                 388 
               
               
                   
               
               
                 797458 
                 N/A 
                 N/A 
                 CCAAAGACTGTTCTAA 
                 44 
                 15749 
                 15764 
                 389 
               
               
                   
               
               
                 797459 
                 N/A 
                 N/A 
                 ACCCACCCCGCCTCCC 
                 100 
                 15769 
                 15784 
                 390 
               
               
                   
               
               
                 797460 
                 N/A 
                 N/A 
                 TTAGAATCTCCAACTC 
                 52 
                 15804 
                 15819 
                 391 
               
               
                   
               
               
                 797461 
                 N/A 
                 N/A 
                 GGTCAGGAAAGGAGCG 
                 22 
                 16629 
                 16644 
                 392 
               
               
                   
               
               
                 797462 
                 N/A 
                 N/A 
                 GGTGTTATTTTAATTA 
                 84 
                 16730 
                 16745 
                 393 
               
               
                   
               
               
                 797463 
                 N/A 
                 N/A 
                 AAAAGCTTGGGCACCA 
                 27 
                 16749 
                 16764 
                 394 
               
               
                   
               
               
                 797464 
                 N/A 
                 N/A 
                 CAAGAGCTGGGACTAG 
                 65 
                 17403 
                 17418 
                 395 
               
               
                   
               
               
                 797465 
                 N/A 
                 N/A 
                 AAAGAATGAGTGATCT 
                 65 
                 17676 
                 17691 
                 396 
               
               
                   
               
               
                 797466 
                 N/A 
                 N/A 
                 GTCAACAAGCATTTCC 
                 15 
                 18034 
                 18049 
                 397 
               
               
                   
               
               
                 797467 
                 N/A 
                 N/A 
                 GGCATTTTTTTAGTCA 
                 59 
                 18046 
                 18061 
                 398 
               
               
                   
               
               
                 797468 
                 N/A 
                 N/A 
                 CAAGATCCATGCTTCC 
                 14 
                 18218 
                 18233 
                 399 
               
               
                   
               
               
                 797469 
                 N/A 
                 N/A 
                 GGATGATGTGATACAT 
                 9 
                 18734 
                 18749 
                 400 
               
               
                   
               
               
                 797470 
                 N/A 
                 N/A 
                 CAATCTAAGAAATAGG 
                 24 
                 18757 
                 18772 
                 401 
               
               
                   
               
               
                 797471 
                 N/A 
                 N/A 
                 TCCAAATGCCTAGAAC 
                 16 
                 18859 
                 18874 
                 402 
               
               
                   
               
               
                 797472 
                 N/A 
                 N/A 
                 GGCGGACTCAGGCTTA 
                 75 
                 19484 
                 19499 
                 403 
               
               
                   
               
               
                 797473 
                 N/A 
                 N/A 
                 TGACAGTTAGAGGAAC 
                 30 
                 19515 
                 19530 
                 404 
               
               
                   
               
               
                 797474 
                 N/A 
                 N/A 
                 GGAAAGCACAGGTGTC 
                 29 
                 19535 
                 19550 
                 405 
               
               
                   
                   
                   
                   
                   
                 19617 
                 19632 
                   
               
               
                   
               
               
                 797475 
                 N/A 
                 N/A 
                 AGGGAAAGCACAGGTG 
                 41 
                 19537 
                 19552 
                 406 
               
               
                   
                   
                   
                   
                   
                 19619 
                 19634 
                   
               
               
                   
               
               
                 797476 
                 N/A 
                 N/A 
                 GCATAGGGAAAGCACA 
                 33 
                 19541 
                 19556 
                 407 
               
               
                   
                   
                   
                   
                   
                 19623 
                 19638 
                   
               
               
                   
               
               
                 797477 
                 N/A 
                 N/A 
                 GGGCATAGGGAAAGCA 
                 33 
                 19543 
                 19558 
                 408 
               
               
                   
                   
                   
                   
                   
                 19625 
                 19640 
                   
               
               
                   
               
               
                 797478 
                 N/A 
                 N/A 
                 GAGGGCATAGGGAAAG 
                 60 
                 19545 
                 19560 
                 409 
               
               
                   
                   
                   
                   
                   
                 19627 
                 19642 
                   
               
               
                   
               
               
                 797479 
                 N/A 
                 N/A 
                 GGTGAGGGCATAGGGA 
                 40 
                 19548 
                 19563 
                 410 
               
               
                   
                   
                   
                   
                   
                 19630 
                 19645 
                   
               
               
                   
               
               
                 797480 
                 N/A 
                 N/A 
                 ATCGGGTGAGGGCATA 
                 34 
                 19552 
                 19567 
                 411 
               
               
                   
                   
                   
                   
                   
                 19634 
                 19649 
                   
               
               
                   
               
               
                 797481 
                 N/A 
                 N/A 
                 ACAGAGCAAAGGGAGG 
                 24 
                 19569 
                 19584 
                 412 
               
               
                   
                   
                   
                   
                   
                 19652 
                 19667 
                   
               
               
                   
               
               
                 797482 
                 N/A 
                 N/A 
                 ACACAGAGCAAAGGGA 
                 45 
                 19571 
                 19586 
                 413 
               
               
                   
                   
                   
                   
                   
                 19654 
                 19669 
                   
               
               
                   
               
               
                 797483 
                 N/A 
                 N/A 
                 GCACACAGAGCAAAGG 
                 19 
                 19573 
                 19588 
                 414 
               
               
                   
                   
                   
                   
                   
                 19656 
                 19671 
                   
               
               
                   
               
               
                 797484 
                 N/A 
                 N/A 
                 AGGCACACAGAGCAAA 
                 25 
                 19575 
                 19590 
                 415 
               
               
                   
                   
                   
                   
                   
                 19658 
                 19673 
                   
               
               
                   
               
               
                 797485 
                 N/A 
                 N/A 
                 AGGCAGGCACACAGAG 
                 26 
                 19579 
                 19594 
                 416 
               
               
                   
                   
                   
                   
                   
                 19662 
                 19677 
                   
               
               
                   
               
               
                 797486 
                 N/A 
                 N/A 
                 GGGGAGGCAGGCACAC 
                 46 
                 19583 
                 19598 
                 417 
               
               
                   
                   
                   
                   
                   
                 19666 
                 19681 
                   
               
               
                   
               
               
                 797487 
                 N/A 
                 N/A 
                 GCACAGGTGTCCATGG 
                 34 
                 19612 
                 19627 
                 418 
               
               
                   
               
               
                 797488 
                 N/A 
                 N/A 
                 GGAATAGTTACATGTG 
                 10 
                 19866 
                 19881 
                 419 
               
               
                   
               
               
                 797489 
                 N/A 
                 N/A 
                 ACCCGATAGCTGGTTG 
                 19 
                 20416 
                 20431 
                 420 
               
               
                   
               
               
                 797490 
                 N/A 
                 N/A 
                 TCACACTATTAATTAG 
                 89 
                 20435 
                 20450 
                 421 
               
               
                   
               
               
                 797491 
                 N/A 
                 N/A 
                 ACTGAACGATTTTAAA 
                 64 
                 20606 
                 20621 
                 422 
               
               
                   
               
               
                 797492 
                 N/A 
                 N/A 
                 CCATGCTAAGGAGTAC 
                 30 
                 20650 
                 20665 
                 423 
               
               
                   
               
               
                   
               
               
                 797493 
                 N/A 
                 N/A 
                 GTACAGGGTTTCTTTT 
                 62 
                 20864 
                 20879 
                 424 
               
               
                   
                   
                   
                   
                   
                 27928 
                 27943 
                   
               
               
                   
               
               
                 797494 
                 N/A 
                 N/A 
                 TTAAATGGTGTGACCA 
                 10 
                 21648 
                 21663 
                 425 
               
               
                   
               
               
                 797495 
                 N/A 
                 N/A 
                 ACGATTACAGGGATTC 
                 9 
                 21751 
                 21766 
                 426 
               
               
                   
               
               
                 797496 
                 N/A 
                 N/A 
                 AGCTGTATTAGCTCAC 
                 71 
                 21771 
                 21786 
                 427 
               
               
                   
               
               
                 797497 
                 N/A 
                 N/A 
                 GTTACTTACTTAATCT 
                 17 
                 21895 
                 21910 
                 428 
               
               
                   
               
               
                 797498 
                 N/A 
                 N/A 
                 AACAAGTATTAGATGT 
                 93 
                 21923 
                 21938 
                 429 
               
               
                   
               
               
                 797499 
                 N/A 
                 N/A 
                 GCACAGACTCCAGAAT 
                 41 
                 21970 
                 21985 
                 430 
               
               
                   
               
               
                 797500 
                 N/A 
                 N/A 
                 CAGTATAATGTGATGG 
                 5 
                 22302 
                 22317 
                 431 
               
               
                   
               
               
                 797501 
                 N/A 
                 N/A 
                 GAGATACACACTAAGC 
                 5 
                 22344 
                 22359 
                 432 
               
               
                   
               
               
                 797502 
                 N/A 
                 N/A 
                 CAGCGGTGGAGAAACA 
                 31 
                 22750 
                 22765 
                 433 
               
               
                   
               
               
                 797503 
                 N/A 
                 N/A 
                 ATGGAAAGCAGGCACA 
                 8 
                 22774 
                 22789 
                 434 
               
               
                   
               
               
                 797504 
                 N/A 
                 N/A 
                 AAGTATAATGGTGGGT 
                 32 
                 22799 
                 22814 
                 435 
               
               
                   
               
               
                 797505 
                 N/A 
                 N/A 
                 TAAAATGTAGGATGAT 
                 86 
                 23033 
                 23048 
                 436 
               
               
                   
               
               
                 797506 
                 N/A 
                 N/A 
                 ACTAAAGAGAAGAGGG 
                 68 
                 23136 
                 23151 
                 437 
               
               
                   
               
               
                 797507 
                 N/A 
                 N/A 
                 AGCAAATCACAGGTTC 
                 5 
                 23303 
                 23318 
                 438 
               
               
                   
               
               
                 797508 
                 N/A 
                 N/A 
                 CAGTACCAAGTGTTTC 
                 17 
                 23442 
                 23457 
                 439 
               
               
                   
               
               
                 797509 
                 N/A 
                 N/A 
                 AGGAGATAGAGGAGAT 
                 39 
                 23589 
                 23604 
                 440 
               
               
                   
               
               
                 797510 
                 N/A 
                 N/A 
                 CTTAAACTCCTACAGG 
                 59 
                 24017 
                 24032 
                 441 
               
               
                   
               
               
                 797511 
                 N/A 
                 N/A 
                 AGCTCAAGGTAAGTAC 
                 98 
                 24277 
                 24292 
                 442 
               
               
                   
               
               
                 797512 
                 N/A 
                 N/A 
                 TCCCAGTCAGCATCCT 
                 89 
                 24733 
                 24748 
                 443 
               
               
                   
               
               
                 797513 
                 N/A 
                 N/A 
                 CCCCAGGGTCCACCCT 
                 75 
                 24902 
                 24917 
                 444 
               
               
                   
               
               
                 797514 
                 N/A 
                 N/A 
                 CTCACATACACAACCC 
                 64 
                 25321 
                 25336 
                 445 
               
               
                   
               
               
                 797515 
                 N/A 
                 N/A 
                 CTGCAGGTTGTTTTTA 
                 26 
                 26098 
                 26113 
                 446 
               
               
                   
                   
                   
                   
                   
                 26434 
                 26449 
                   
               
               
                   
               
               
                 797516 
                 N/A 
                 N/A 
                 AATGGATACAGTATGT 
                 98 
                 26688 
                 26703 
                 447 
               
               
                   
               
               
                 797517 
                 N/A 
                 N/A 
                 AGTCAAAAGGAAAGAG 
                 25 
                 26848 
                 26863 
                 448 
               
               
                   
               
               
                 797518 
                 N/A 
                 N/A 
                 CAAACAACTCAACTGT 
                 24 
                 26864 
                 26879 
                 449 
               
               
                   
               
               
                 797519 
                 N/A 
                 N/A 
                 AGCATGAACTCCAGGA 
                 11 
                 26959 
                 26974 
                 450 
               
               
                   
               
               
                 797520 
                 N/A 
                 N/A 
                 AAATGCAACAGACTGT 
                 30 
                 27542 
                 27557 
                 451 
               
               
                   
               
               
                 797521 
                 N/A 
                 N/A 
                 ACCCAAATCCCTACCA 
                 46 
                 27624 
                 27639 
                 452 
               
               
                   
               
               
                 797522 
                 N/A 
                 N/A 
                 GATATAAATTATCTCA 
                 88 
                 27780 
                 27795 
                 453 
               
               
                   
               
               
                 797523 
                 N/A 
                 N/A 
                 GCTAATGAGTACAAGG 
                 9 
                 28243 
                 28258 
                 454 
               
               
                   
               
               
                 797524 
                 N/A 
                 N/A 
                 ACCATACGGATGAACC 
                 13 
                 28742 
                 28757 
                 455 
               
               
                   
               
               
                 797525 
                 N/A 
                 N/A 
                 TCAAAAAAGGTTAAAC 
                 90 
                 28996 
                 29011 
                 456 
               
               
                   
               
               
                 797526 
                 N/A 
                 N/A 
                 ATGTACAATGGTGATG 
                 34 
                 29040 
                 29055 
                 457 
               
               
                   
               
               
                 797527 
                 N/A 
                 N/A 
                 AAAGAAAAATGGGAAC 
                 87 
                 29535 
                 29550 
                 458 
               
               
                   
                   
                   
                   
                   
                 29852 
                 29867 
                   
               
               
                   
               
               
                 797528 
                 N/A 
                 N/A 
                 TTATAATGATGCCTTA 
                 78 
                 30466 
                 30481 
                 459 
               
               
                   
               
               
                 797529 
                 N/A 
                 N/A 
                 CCCAAGAGGTCTCCCA 
                 58 
                 30522 
                 30537 
                 460 
               
               
                   
               
               
                 797530 
                 N/A 
                 N/A 
                 CATAATACCCAGAGAA 
                 57 
                 30895 
                 30910 
                 461 
               
               
                   
               
               
                 797531 
                 N/A 
                 N/A 
                 CACTCATACACATAAT 
                 68 
                 30905 
                 30920 
                 462 
               
               
                   
               
               
                 797532 
                 N/A 
                 N/A 
                 TGTCACCCAGAGAAAA 
                 66 
                 31564 
                 31579 
                 463 
               
               
                   
               
               
                 797533 
                 N/A 
                 N/A 
                 AGGTACATTGACGATG 
                 85 
                 31720 
                 31735 
                 464 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 SEQ 
                 SEQ 
                   
                   
                 SEQ 
                 SEQ 
                   
               
               
                   
                 ID: 1 
                 ID: 1 
                   
                 α-ENaC 
                 ID: 2 
                 ID 2: 
                 SEQ 
               
               
                 Compound 
                 Start 
                 Stop 
                   
                 (% 
                 Start 
                 Stop 
                 ID 
               
               
                 Number 
                 Site 
                 Site 
                 Sequence 
                 control) 
                 Site 
                 Site 
                 NO 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 797192 
                 1578 
                 1593 
                 AAGATGTAGGCACAGC 
                 30 
                 25492 
                 25507 
                 132 
               
               
                   
               
               
                 797235 
                 2045 
                 2060 
                 GGCTTCGGAACCTTCG 
                 18 
                 31874 
                 31889 
                 166 
               
               
                   
               
               
                 797507 
                 N/A 
                 N/A 
                 AGCAAATCACAGGTTC 
                 20 
                 23303 
                 23318 
                 438 
               
               
                   
               
               
                 826070 
                 1 
                 16 
                 GACGCAGACAGGCAAG 
                 76 
                 4262 
                 4277 
                 465 
               
               
                   
               
               
                 826071 
                 5 
                 20 
                 TTTAGACGCAGACAGG 
                 67 
                 4266 
                 4281 
                 466 
               
               
                   
               
               
                 826090 
                 52 
                 67 
                 GTGAACTGGGAGTACT 
                 91 
                 4313 
                 4328 
                 467 
               
               
                   
               
               
                 826091 
                 53 
                 68 
                 GGTGAACTGGGAGTAC 
                 57 
                 4314 
                 4329 
                 468 
               
               
                   
               
               
                 826110 
                 145 
                 160 
                 GCATCTCAATTAAAGG 
                 86 
                 4406 
                 4421 
                 469 
               
               
                   
               
               
                 826111 
                 163 
                 178 
                 GCGACAGGAATCTCAT 
                 66 
                 4424 
                 4439 
                 470 
               
               
                   
               
               
                 826130 
                 195 
                 210 
                 GGAGCCCGCCCGCTGG 
                 60 
                 4456 
                 4471 
                 471 
               
               
                   
               
               
                 826131 
                 216 
                 231 
                 CAGGTGCAGCGGCCTG 
                 79 
                 4477 
                 4492 
                 472 
               
               
                   
               
               
                 826150 
                 282 
                 297 
                 CCCTCCATGAGACCTG 
                 30 
                 5210 
                 5225 
                 473 
               
               
                   
               
               
                 826151 
                 283 
                 298 
                 CCCCTCCATGAGACCT 
                 19 
                 5211 
                 5226 
                 474 
               
               
                   
               
               
                 826170 
                 354 
                 369 
                 TCACGCTTGTTCCCCT 
                 25 
                 5282 
                 5297 
                 475 
               
               
                   
               
               
                 826171 
                 355 
                 370 
                 CTCACGCTTGTTCCCC 
                 17 
                 5283 
                 5298 
                 476 
               
               
                   
               
               
                 826190 
                 439 
                 454 
                 GTAGGAGCGGTGGAAC 
                 88 
                 5367 
                 5382 
                 477 
               
               
                   
               
               
                 826191 
                 441 
                 456 
                 CGGTAGGAGCGGTGGA 
                 64 
                 5369 
                 5384 
                 478 
               
               
                   
               
               
                 826209 
                 565 
                 580 
                 CATGCCAAAGGTGCAG 
                 43 
                 5493 
                 5508 
                 479 
               
               
                   
               
               
                 826210 
                 566 
                 581 
                 TCATGCCAAAGGTGCA 
                 45 
                 5494 
                 5509 
                 480 
               
               
                   
               
               
                 826229 
                 606 
                 621 
                 CTGAAGTACTCTCCGA 
                 23 
                 5534 
                 5549 
                 481 
               
               
                   
               
               
                 826230 
                 607 
                 622 
                 GCTGAAGTACTCTCCG 
                 26 
                 5535 
                 5550 
                 482 
               
               
                   
               
               
                 826249 
                 702 
                 717 
                 ATTTCCGGGTACCTGT 
                 33 
                 N/A 
                 N/A 
                 483 
               
               
                   
               
               
                 826250 
                 703 
                 718 
                 AATTTCCGGGTACCTG 
                 40 
                 16288 
                 16303 
                 484 
               
               
                   
               
               
                 826267 
                 791 
                 806 
                 CGGCCACGAGAGTGGT 
                 62 
                 16376 
                 16391 
                 485 
               
               
                   
               
               
                 826268 
                 792 
                 807 
                 CCGGCCACGAGAGTGG 
                 58 
                 16377 
                 16392 
                 486 
               
               
                   
               
               
                 826287 
                 828 
                 843 
                 GGCAGAGTCCCCCGCA 
                 35 
                 16413 
                 16428 
                 487 
               
               
                   
               
               
                 826288 
                 830 
                 845 
                 GCGGCAGAGTCCCCCG 
                 38 
                 16415 
                 16430 
                 488 
               
               
                   
               
               
                 826307 
                 914 
                 929 
                 TGTTGTCCCGCAAGCT 
                 42 
                 16499 
                 16514 
                 489 
               
               
                   
               
               
                 826308 
                 916 
                 931 
                 GTTGTTGTCCCGCAAG 
                 26 
                 16501 
                 16516 
                 490 
               
               
                   
               
               
                 826325 
                 979 
                 994 
                 GTCCGATTTGTTCTGG 
                 31 
                 17765 
                 17780 
                 491 
               
               
                   
               
               
                 826326 
                 980 
                 995 
                 AGTCCGATTTGTTCTG 
                 58 
                 17766 
                 17781 
                 492 
               
               
                   
               
               
                 826345 
                 1017 
                 1032 
                 ACCGCATCCACCCCTG 
                 42 
                 17803 
                 17818 
                 493 
               
               
                   
               
               
                 826346 
                 1018 
                 1033 
                 CACCGCATCCACCCCT 
                 41 
                 17804 
                 17819 
                 494 
               
               
                   
               
               
                 826365 
                 1113 
                 1128 
                 AAGATGAAGTTGCCCA 
                 41 
                 17899 
                 17914 
                 495 
               
               
                   
               
               
                 826366 
                 1115 
                 1130 
                 CGAAGATGAAGTTGCC 
                 55 
                 17901 
                 17916 
                 496 
               
               
                   
               
               
                 826385 
                 1162 
                 1177 
                 GTGAGAGTAATTCGCC 
                 65 
                 N/A 
                 N/A 
                 497 
               
               
                   
               
               
                 826386 
                 1164 
                 1179 
                 AAGTGAGAGTAATTCG 
                 29 
                 N/A 
                 N/A 
                 498 
               
               
                   
               
               
                 826405 
                 1281 
                 1296 
                 TGCTCTGCGCGCAGCA 
                 62 
                 24578 
                 24593 
                 499 
               
               
                   
               
               
                 826406 
                 1282 
                 1297 
                 CTGCTCTGCGCGCAGC 
                 58 
                 24579 
                 24594 
                 500 
               
               
                   
               
               
                 826425 
                 1354 
                 1369 
                 GGCAGGTTCATCCTGC 
                 140 
                 24651 
                 24666 
                 501 
               
               
                   
               
               
                 826426 
                 1356 
                 1371 
                 AAGGCAGGTTCATCCT 
                 63 
                 24653 
                 24668 
                 502 
               
               
                   
               
               
                 826445 
                 1405 
                 1420 
                 GATGGAGGTCTCCACG 
                 67 
                 24702 
                 24717 
                 503 
               
               
                   
               
               
                 826446 
                 1416 
                 1431 
                 TTCCTCATGCTGATGG 
                 24 
                 24713 
                 24728 
                 504 
               
               
                   
               
               
                 826465 
                 1497 
                 1512 
                 GGGTAAAGGTTCTCAA 
                 25 
                 25217 
                 25232 
                 505 
               
               
                   
               
               
                 826466 
                 1500 
                 1515 
                 GAAGGGTAAAGGTTCT 
                 63 
                 25220 
                 25235 
                 506 
               
               
                   
               
               
                 826485 
                 1579 
                 1594 
                 GAAGATGTAGGCACAG 
                 32 
                 25493 
                 25508 
                 507 
               
               
                   
               
               
                 826504 
                 1656 
                 1671 
                 TGGAGCTTATAGTAGC 
                 30 
                 30602 
                 30617 
                 508 
               
               
                   
               
               
                 826505 
                 1661 
                 1676 
                 CAACCTGGAGCTTATA 
                 42 
                 30607 
                 30622 
                 509 
               
               
                   
               
               
                 826524 
                 1733 
                 1748 
                 AGAGCTGGTAGCTGGT 
                 67 
                 30784 
                 30799 
                 510 
               
               
                   
               
               
                 826525 
                 1736 
                 1751 
                 CAGAGAGCTGGTAGCT 
                 75 
                 30787 
                 30802 
                 511 
               
               
                   
               
               
                 826564 
                 1992 
                 2007 
                 ACGAGCTCAGCCATCT 
                 57 
                 31821 
                 31836 
                 512 
               
               
                   
               
               
                 826565 
                 1993 
                 2008 
                 GACGAGCTCAGCCATC 
                 16 
                 31822 
                 31837 
                 513 
               
               
                   
               
               
                 826584 
                 2046 
                 2061 
                 CGGCTTCGGAACCTTC 
                 23 
                 31875 
                 31890 
                 514 
               
               
                   
               
               
                 826603 
                 2216 
                 2231 
                 CCAGGGTGGCATAGGC 
                 28 
                 32045 
                 32060 
                 515 
               
               
                   
               
               
                 826604 
                 2217 
                 2232 
                 CCCAGGGTGGCATAGG 
                 42 
                 32046 
                 32061 
                 516 
               
               
                   
               
               
                 826623 
                 2360 
                 2375 
                 CCTTCAATCTTGCCAG 
                 31 
                 32189 
                 32204 
                 517 
               
               
                   
               
               
                 826624 
                 2390 
                 2405 
                 TGGGCGGCTCTGAGAG 
                 49 
                 32219 
                 32234 
                 518 
               
               
                   
               
               
                 826643 
                 2464 
                 2479 
                 ATCAGCTACTGTTCTT 
                 32 
                 32293 
                 32308 
                 519 
               
               
                   
               
               
                 826644 
                 2476 
                 2491 
                 CTGGGCAGCTTCATCA 
                 33 
                 32305 
                 32320 
                 520 
               
               
                   
               
               
                 826662 
                 2575 
                 2590 
                 GCTGATCCAAGGGAAA 
                 35 
                 32404 
                 32419 
                 521 
               
               
                   
               
               
                 826681 
                 2610 
                 2625 
                 GGAAAGTTCCTTGTCA 
                 40 
                 32439 
                 32454 
                 522 
               
               
                   
               
               
                 826682 
                 2612 
                 2627 
                 TAGGAAAGTTCCTTGT 
                 32 
                 32441 
                 32456 
                 523 
               
               
                   
               
               
                 826701 
                 2679 
                 2694 
                 GGCAGGAAACCCGTGC 
                 74 
                 32508 
                 32523 
                 524 
               
               
                   
               
               
                 826702 
                 2681 
                 2696 
                 TGGGCAGGAAACCCGT 
                 64 
                 32510 
                 32525 
                 525 
               
               
                   
               
               
                 826721 
                 2832 
                 2847 
                 AGCCCTCGGGAGTCAG 
                 27 
                 32661 
                 32676 
                 526 
               
               
                   
               
               
                 826722 
                 2835 
                 2850 
                 CCTAGCCCTCGGGAGT 
                 62 
                 32664 
                 32679 
                 527 
               
               
                   
               
               
                 826741 
                 2896 
                 2911 
                 GCACGGGTATGAGGCT 
                 69 
                 32725 
                 32740 
                 528 
               
               
                   
               
               
                 826742 
                 2949 
                 2964 
                 TGTAGAGGTATGAAAG 
                 31 
                 32778 
                 32793 
                 529 
               
               
                   
               
               
                 826761 
                 3012 
                 3027 
                 ATAGGAGTTCTCTGGC 
                 16 
                 32841 
                 32856 
                 530 
               
               
                   
               
               
                 826780 
                 3101 
                 3116 
                 GAAGGCGGAGTGATCA 
                 65 
                 32930 
                 32945 
                 531 
               
               
                   
               
               
                 826781 
                 3117 
                 3132 
                 CTACGGGAGCCCAGGA 
                 22 
                 32946 
                 32961 
                 532 
               
               
                   
               
               
                 826799 
                 3143 
                 3158 
                 AACACTCCAGCAGATG 
                 40 
                 32972 
                 32987 
                 533 
               
               
                   
               
               
                 826800 
                 3145 
                 3160 
                 GCAACACTCCAGCAGA 
                 19 
                 32974 
                 32989 
                 534 
               
               
                   
               
               
                 826817 
                 3212 
                 3227 
                 GACCGCAAGGCACTTA 
                 51 
                 33041 
                 33056 
                 535 
               
               
                   
               
               
                 826818 
                 3213 
                 3228 
                 TGACCGCAAGGCACTT 
                 19 
                 33042 
                 33057 
                 536 
               
               
                   
               
               
                 826836 
                 3235 
                 3250 
                 TAAACGGGCAAGATTC 
                 41 
                 33064 
                 33079 
                 537 
               
               
                   
               
               
                 826837 
                 3236 
                 3251 
                 ATAAACGGGCAAGATT 
                 71 
                 33065 
                 33080 
                 538 
               
               
                   
               
               
                 826856 
                 N/A 
                 N/A 
                 TTGTCCTAATGAAGAA 
                 95 
                 2680 
                 2695 
                 539 
               
               
                   
               
               
                 826857 
                 N/A 
                 N/A 
                 TCCCCTTGGAAGGGAC 
                 66 
                 2713 
                 2728 
                 540 
               
               
                   
               
               
                 826876 
                 N/A 
                 N/A 
                 AGGATCTGTGTCTCAG 
                 19 
                 4815 
                 4830 
                 541 
               
               
                   
               
               
                 826877 
                 N/A 
                 N/A 
                 GTCCTAGCACCTCCCT 
                 15 
                 4868 
                 4883 
                 542 
               
               
                   
               
               
                 826896 
                 N/A 
                 N/A 
                 CCCTAGAACGGCCTCT 
                 27 
                 4920 
                 4935 
                 543 
               
               
                   
               
               
                 826897 
                 N/A 
                 N/A 
                 CTTCCCTAGAACGGCC 
                 19 
                 4923 
                 4938 
                 544 
               
               
                   
               
               
                 826916 
                 N/A 
                 N/A 
                 ACCCGAGTGAGGCTGC 
                 13 
                 5057 
                 5072 
                 545 
               
               
                   
               
               
                 826917 
                 N/A 
                 N/A 
                 GAACCCGAGTGAGGCT 
                 60 
                 5059 
                 5074 
                 546 
               
               
                   
               
               
                 826936 
                 N/A 
                 N/A 
                 CCACACAGAGCCCGTG 
                 66 
                 2463 
                 2478 
                 547 
               
               
                   
               
               
                 826937 
                 N/A 
                 N/A 
                 AGCCGGGAAGGCCTCC 
                 72 
                 2486 
                 2501 
                 548 
               
               
                   
               
               
                 826956 
                 N/A 
                 N/A 
                 CCCAAAAAGGGCTGGA 
                 66 
                 3294 
                 3309 
                 549 
               
               
                   
               
               
                 826957 
                 N/A 
                 N/A 
                 GCCAAGTGGTGAGCAA 
                 74 
                 3338 
                 3353 
                 550 
               
               
                   
               
               
                 826976 
                 N/A 
                 N/A 
                 GTTGAATCTGGCAGCC 
                 64 
                 4517 
                 4532 
                 551 
               
               
                   
               
               
                 826977 
                 N/A 
                 N/A 
                 TGGGACTGGTTCCTTT 
                 109 
                 4536 
                 4551 
                 552 
               
               
                   
               
               
                 826996 
                 N/A 
                 N/A 
                 TGGCAAAGAGCACCGA 
                 51 
                 6348 
                 6363 
                 553 
               
               
                   
               
               
                 826997 
                 N/A 
                 N/A 
                 CCAGACCCAACATTGG 
                 87 
                 6361 
                 6376 
                 554 
               
               
                   
               
               
                 827016 
                 N/A 
                 N/A 
                 GTCCGTAACGCACCTT 
                 36 
                 6807 
                 6822 
                 555 
               
               
                   
               
               
                 827017 
                 N/A 
                 N/A 
                 CTTTCTTAGTCCGTAA 
                 41 
                 6815 
                 6830 
                 556 
               
               
                   
               
               
                 827036 
                 N/A 
                 N/A 
                 GACTACAAGGTCAAGT 
                 77 
                 7660 
                 7675 
                 557 
               
               
                   
               
               
                 827056 
                 N/A 
                 N/A 
                 ATTCACTGCGCTCCCG 
                 44 
                 8755 
                 8770 
                 558 
               
               
                   
               
               
                 827057 
                 N/A 
                 N/A 
                 TCGGTAGGAGTCATTC 
                 15 
                 8767 
                 8782 
                 559 
               
               
                   
               
               
                 827076 
                 N/A 
                 N/A 
                 ACAGAAGAGCCCATGC 
                 55 
                 9484 
                 9499 
                 560 
               
               
                   
               
               
                 827077 
                 N/A 
                 N/A 
                 TCTTACCCCGGTGGCC 
                 53 
                 9507 
                 9522 
                 561 
               
               
                   
               
               
                 827096 
                 N/A 
                 N/A 
                 CCCTACGCGCCTCCCT 
                 92 
                 10629 
                 10644 
                 562 
               
               
                   
               
               
                 827097 
                 N/A 
                 N/A 
                 CTGCCACCCTACGCGC 
                 53 
                 10635 
                 10650 
                 563 
               
               
                   
               
               
                 827116 
                 N/A 
                 N/A 
                 GCCTACCGCATGAAGC 
                 42 
                 11082 
                 11097 
                 564 
               
               
                   
               
               
                 827117 
                 N/A 
                 N/A 
                 GGGCATAACACTAGAT 
                 42 
                 11100 
                 11115 
                 565 
               
               
                   
               
               
                 827136 
                 N/A 
                 N/A 
                 TGGACAAGGTTTGACA 
                 65 
                 11623 
                 11638 
                 566 
               
               
                   
               
               
                 827137 
                 N/A 
                 N/A 
                 GGTTACACCCCCGGCG 
                 60 
                 11650 
                 11665 
                 567 
               
               
                   
               
               
                 827156 
                 N/A 
                 N/A 
                 GCTACATTAACCACCG 
                 31 
                 12134 
                 12149 
                 568 
               
               
                   
               
               
                 827157 
                 N/A 
                 N/A 
                 TTGAAAGAGCCCCCAC 
                 26 
                 12166 
                 12181 
                 569 
               
               
                   
               
               
                 827176 
                 N/A 
                 N/A 
                 CAGGAACTATGGTATT 
                 18 
                 12690 
                 12705 
                 570 
               
               
                   
               
               
                 827177 
                 N/A 
                 N/A 
                 GCAATCATAGCTAGCA 
                 116 
                 12729 
                 12744 
                 571 
               
               
                   
               
               
                 827196 
                 N/A 
                 N/A 
                 TTTATGAACAGACCTA 
                 19 
                 14134 
                 14149 
                 572 
               
               
                   
               
               
                 827197 
                 N/A 
                 N/A 
                 GTAGGCACTTTATGAA 
                 28 
                 14142 
                 14157 
                 573 
               
               
                   
               
               
                 827215 
                 N/A 
                 N/A 
                 GCATAGATGGTCAACT 
                 43 
                 14438 
                 14453 
                 574 
               
               
                   
               
               
                 827216 
                 N/A 
                 N/A 
                 GGAGAGACAATAGATC 
                 31 
                 14488 
                 14503 
                 575 
               
               
                   
               
               
                 827235 
                 N/A 
                 N/A 
                 GGCTTGAGTGCCGCTT 
                 17 
                 15852 
                 15867 
                 576 
               
               
                   
               
               
                 827236 
                 N/A 
                 N/A 
                 CCGCAGGCGAGTGTCG 
                 63 
                 15878 
                 15893 
                 577 
               
               
                   
               
               
                 827255 
                 N/A 
                 N/A 
                 ACTAATGGGAACTTCC 
                 47 
                 17363 
                 17378 
                 578 
               
               
                   
               
               
                 827256 
                 N/A 
                 N/A 
                 CAAGAGATTTGTCCCA 
                 39 
                 17420 
                 17435 
                 579 
               
               
                   
               
               
                 827275 
                 N/A 
                 N/A 
                 GAGCAGCAGGAGTTCG 
                 45 
                 18126 
                 18141 
                 580 
               
               
                   
               
               
                 827276 
                 N/A 
                 N/A 
                 CCTCAGATCCAGCAGT 
                 50 
                 18147 
                 18162 
                 581 
               
               
                   
               
               
                 827294 
                 N/A 
                 N/A 
                 ATACATCCAGAGTCAC 
                 25 
                 18724 
                 18739 
                 582 
               
               
                   
               
               
                 827295 
                 N/A 
                 N/A 
                 GATACATCCAGAGTCA 
                 31 
                 18725 
                 18740 
                 583 
               
               
                   
               
               
                 827313 
                 N/A 
                 N/A 
                 CTCCGGAAAATAAACG 
                 25 
                 19270 
                 19285 
                 584 
               
               
                   
               
               
                 827314 
                 N/A 
                 N/A 
                 GGAGATGGCTCCGGAA 
                 55 
                 19278 
                 19293 
                 585 
               
               
                   
               
               
                 827333 
                 N/A 
                 N/A 
                 ACCATGGACTTTCTGT 
                 18 
                 19686 
                 19701 
                 586 
               
               
                   
               
               
                 827334 
                 N/A 
                 N/A 
                 AACCATGGACTTTCTG 
                 42 
                 19687 
                 19702 
                 587 
               
               
                   
               
               
                 827352 
                 N/A 
                 N/A 
                 CTAACTGGAATAGTTA 
                 89 
                 19872 
                 19887 
                 588 
               
               
                   
               
               
                 827353 
                 N/A 
                 N/A 
                 ACTAACTGGAATAGTT 
                 60 
                 19873 
                 19888 
                 589 
               
               
                   
               
               
                 827372 
                 N/A 
                 N/A 
                 ATCATAGTATTCAGCC 
                 18 
                 21087 
                 21102 
                 590 
               
               
                   
               
               
                 827373 
                 N/A 
                 N/A 
                 AAAAAGGTGGTGTATC 
                 50 
                 21111 
                 21126 
                 591 
               
               
                   
               
               
                 827391 
                 N/A 
                 N/A 
                 ATTACAGGGATTCATT 
                 26 
                 21748 
                 21763 
                 592 
               
               
                   
               
               
                 827392 
                 N/A 
                 N/A 
                 CGATTACAGGGATTCA 
                 20 
                 21750 
                 21765 
                 593 
               
               
                   
               
               
                 827409 
                 N/A 
                 N/A 
                 GGCCAGTAAGAGTGAA 
                 90 
                 22013 
                 22028 
                 594 
               
               
                   
               
               
                 827410 
                 N/A 
                 N/A 
                 GAATCAGTATAATGTG 
                 11 
                 22306 
                 22321 
                 595 
               
               
                   
               
               
                 827428 
                 N/A 
                 N/A 
                 TGCCCCCATGGAAAGC 
                 54 
                 22781 
                 22796 
                 596 
               
               
                   
               
               
                 827429 
                 N/A 
                 N/A 
                 CTGCCCCCATGGAAAG 
                 32 
                 22782 
                 22797 
                 597 
               
               
                   
               
               
                 827448 
                 N/A 
                 N/A 
                 CTGCAGTAGGACTGCA 
                 80 
                 23326 
                 23341 
                 598 
               
               
                   
               
               
                 827467 
                 N/A 
                 N/A 
                 AAGCAGGGAGCTTCTC 
                 80 
                 24227 
                 24242 
                 599 
               
               
                   
               
               
                 827468 
                 N/A 
                 N/A 
                 CGCCATGGAGCAAGCA 
                 95 
                 24238 
                 24253 
                 600 
               
               
                   
               
               
                 827487 
                 N/A 
                 N/A 
                 TGCAACACTGAGAGGG 
                 45 
                 26035 
                 26050 
                 601 
               
               
                   
               
               
                 827488 
                 N/A 
                 N/A 
                 GGACAATTCCTTGACA 
                 38 
                 26078 
                 26093 
                 602 
               
               
                   
               
               
                 827507 
                 N/A 
                 N/A 
                 GACAATCCGCTGCCTT 
                 15 
                 27116 
                 27131 
                 603 
               
               
                   
               
               
                 827508 
                 N/A 
                 N/A 
                 AGGTAGGGATGGACGC 
                 15 
                 27147 
                 27162 
                 604 
               
               
                   
               
               
                 827527 
                 N/A 
                 N/A 
                 GGGACTTGCTAATGAG 
                 50 
                 28250 
                 28265 
                 605 
               
               
                   
               
               
                 827528 
                 N/A 
                 N/A 
                 TGGGACTTGCTAATGA 
                 48 
                 28251 
                 28266 
                 606 
               
               
                   
               
               
                 827546 
                 N/A 
                 N/A 
                 GTCCACTAACTGATAA 
                 52 
                 28839 
                 28854 
                 607 
               
               
                   
               
               
                 827547 
                 N/A 
                 N/A 
                 GGTGATGTCACTTCGG 
                 12 
                 29031 
                 29046 
                 608 
               
               
                   
               
               
                 827566 
                 N/A 
                 N/A 
                 CACATAATACCCAGAG 
                 61 
                 30897 
                 30912 
                 609 
               
               
                   
               
               
                 827567 
                 N/A 
                 N/A 
                 GGATAGGGTTGTGTCA 
                 57 
                 30925 
                 30940 
                 610 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 SEQ 
                 SEQ 
                   
                   
                 SEQ 
                 SEQ 
                   
               
               
                   
                 ID: 1 
                 ID: 1 
                   
                 α-ENaC 
                 ID: 2 
                 ID 2: 
                 SEQ 
               
               
                 Compound 
                 Start 
                 Stop 
                   
                 (% 
                 Start 
                 Stop 
                 ID 
               
               
                 Number 
                 Site 
                 Site 
                 Sequence 
                 control) 
                 Site 
                 Site 
                 NO 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 797494 
                 N/A 
                 N/A 
                 TTAAATGGTGTGACCA 
                 12 
                 21648 
                 21663 
                 425 
               
               
                   
               
               
                 797524 
                 N/A 
                 N/A 
                 ACCATACGGATGAACC 
                 37 
                 28742 
                 28757 
                 455 
               
               
                   
               
               
                 826074 
                 25 
                 40 
                 GGCGGACTCTGGGCAG 
                 135 
                 4286 
                 4301 
                 611 
               
               
                   
               
               
                 826075 
                 28 
                 43 
                 GAAGGCGGACTCTGGG 
                 121 
                 4289 
                 4304 
                 612 
               
               
                   
               
               
                 826076 
                 29 
                 44 
                 AGAAGGCGGACTCTGG 
                 119 
                 4290 
                 4305 
                 613 
               
               
                   
               
               
                 826077 
                 31 
                 46 
                 TGAGAAGGCGGACTCT 
                 75 
                 4292 
                 4307 
                 614 
               
               
                   
               
               
                 826078 
                 32 
                 47 
                 CTGAGAAGGCGGACTC 
                 70 
                 4293 
                 4308 
                 615 
               
               
                   
               
               
                 826079 
                 33 
                 48 
                 CCTGAGAAGGCGGACT 
                 163 
                 4294 
                 4309 
                 616 
               
               
                   
               
               
                 826081 
                 36 
                 51 
                 GGACCTGAGAAGGCGG 
                 46 
                 4297 
                 4312 
                 617 
               
               
                   
               
               
                 826094 
                 73 
                 88 
                 AAGGAGGGCTCCCGAG 
                 111 
                 4334 
                 4349 
                 618 
               
               
                   
               
               
                 826095 
                 74 
                 89 
                 GAAGGAGGGCTCCCGA 
                 196 
                 4335 
                 4350 
                 619 
               
               
                   
               
               
                 826096 
                 81 
                 96 
                 TCCGAAGGAAGGAGGG 
                 197 
                 4342 
                 4357 
                 620 
               
               
                   
               
               
                 826097 
                 83 
                 98 
                 TTTCCGAAGGAAGGAG 
                 89 
                 4344 
                 4359 
                 621 
               
               
                   
               
               
                 826098 
                 85 
                 100 
                 GTTTTCCGAAGGAAGG 
                 125 
                 4346 
                 4361 
                 622 
               
               
                   
               
               
                 826099 
                 88 
                 103 
                 GGAGTTTTCCGAAGGA 
                 149 
                 4349 
                 4364 
                 623 
               
               
                   
               
               
                 826100 
                 91 
                 106 
                 CCGGGAGTTTTCCGAA 
                 59 
                 4352 
                 4367 
                 624 
               
               
                   
               
               
                 826101 
                 92 
                 107 
                 GCCGGGAGTTTTCCGA 
                 98 
                 4353 
                 4368 
                 625 
               
               
                   
               
               
                 826114 
                 167 
                 182 
                 GGAAGCGACAGGAATC 
                 162 
                 4428 
                 4443 
                 626 
               
               
                   
               
               
                 826115 
                 169 
                 184 
                 ATGGAAGCGACAGGAA 
                 118 
                 4430 
                 4445 
                 627 
               
               
                   
               
               
                 826116 
                 170 
                 185 
                 GATGGAAGCGACAGGA 
                 145 
                 4431 
                 4446 
                 628 
               
               
                   
               
               
                 826117 
                 171 
                 186 
                 GGATGGAAGCGACAGG 
                 143 
                 4432 
                 4447 
                 629 
               
               
                   
               
               
                 826118 
                 172 
                 187 
                 GGGATGGAAGCGACAG 
                 82 
                 4433 
                 4448 
                 630 
               
               
                   
               
               
                 826119 
                 175 
                 190 
                 CCAGGGATGGAAGCGA 
                 95 
                 4436 
                 4451 
                 631 
               
               
                   
               
               
                 826120 
                 176 
                 191 
                 GCCAGGGATGGAAGCG 
                 54 
                 4437 
                 4452 
                 632 
               
               
                   
               
               
                 826121 
                 180 
                 195 
                 GCCGGCCAGGGATGGA 
                 74 
                 4441 
                 4456 
                 633 
               
               
                   
               
               
                 826134 
                 226 
                 241 
                 GTTCCCCTGACAGGTG 
                 74 
                 N/A 
                 N/A 
                 634 
               
               
                   
               
               
                 826135 
                 228 
                 243 
                 TTGTTCCCCTGACAGG 
                 68 
                 N/A 
                 N/A 
                 635 
               
               
                   
               
               
                 826136 
                 229 
                 244 
                 CTTGTTCCCCTGACAG 
                 102 
                 N/A 
                 N/A 
                 636 
               
               
                   
               
               
                 826137 
                 230 
                 245 
                 GCTTGTTCCCCTGACA 
                 15 
                 N/A 
                 N/A 
                 637 
               
               
                   
               
               
                 826138 
                 232 
                 247 
                 CAGCTTGTTCCCCTGA 
                 30 
                 N/A 
                 N/A 
                 638 
               
               
                   
               
               
                 826139 
                 233 
                 248 
                 CCAGCTTGTTCCCCTG 
                 77 
                 N/A 
                 N/A 
                 639 
               
               
                   
               
               
                 826140 
                 249 
                 264 
                 CTAGGGTCCTGCTCCT 
                 55 
                 5177 
                 5192 
                 640 
               
               
                   
               
               
                 826141 
                 254 
                 269 
                 GAGGTCTAGGGTCCTG 
                 44 
                 5182 
                 5197 
                 641 
               
               
                   
               
               
                 826154 
                 292 
                 307 
                 CAGCTTGTTCCCCTCC 
                 33 
                 5220 
                 5235 
                 642 
               
               
                   
               
               
                 826155 
                 310 
                 325 
                 GCTAGAGTCCTGCTCC 
                 82 
                 5238 
                 5253 
                 643 
               
               
                   
               
               
                 826156 
                 312 
                 327 
                 GGGCTAGAGTCCTGCT 
                 134 
                 5240 
                 5255 
                 644 
               
               
                   
               
               
                 826157 
                 315 
                 330 
                 GGAGGGCTAGAGTCCT 
                 91 
                 5243 
                 5258 
                 645 
               
               
                   
               
               
                 826158 
                 320 
                 335 
                 ACTGTGGAGGGCTAGA 
                 50 
                 5248 
                 5263 
                 646 
               
               
                   
               
               
                 826159 
                 321 
                 336 
                 GACTGTGGAGGGCTAG 
                 59 
                 5249 
                 5264 
                 647 
               
               
                   
               
               
                 826160 
                 322 
                 337 
                 GGACTGTGGAGGGCTA 
                 34 
                 5250 
                 5265 
                 648 
               
               
                   
               
               
                 826161 
                 331 
                 346 
                 CCCTGGAGTGGACTGT 
                 50 
                 5259 
                 5274 
                 649 
               
               
                   
               
               
                 826174 
                 360 
                 375 
                 TGCTCCTCACGCTTGT 
                 27 
                 5288 
                 5303 
                 650 
               
               
                   
               
               
                 826175 
                 362 
                 377 
                 CCTGCTCCTCACGCTT 
                 33 
                 5290 
                 5305 
                 651 
               
               
                   
               
               
                 826176 
                 363 
                 378 
                 CCCTGCTCCTCACGCT 
                 51 
                 5291 
                 5306 
                 652 
               
               
                   
               
               
                 826177 
                 386 
                 401 
                 GCGCCGCAGGTTCGGG 
                 51 
                 5314 
                 5329 
                 653 
               
               
                   
               
               
                 826178 
                 405 
                 420 
                 TCCGCCGTGGGCTGCT 
                 52 
                 5333 
                 5348 
                 654 
               
               
                   
               
               
                 826179 
                 407 
                 422 
                 CCTCCGCCGTGGGCTG 
                 50 
                 5335 
                 5350 
                 655 
               
               
                   
               
               
                 826180 
                 411 
                 426 
                 TCCTCCTCCGCCGTGG 
                 31 
                 5339 
                 5354 
                 656 
               
               
                   
               
               
                 826181 
                 422 
                 437 
                 CGATCAGGGCCTCCTC 
                 33 
                 5350 
                 5365 
                 657 
               
               
                   
               
               
                 826194 
                 446 
                 461 
                 GCTCTCGGTAGGAGCG 
                 44 
                 5374 
                 5389 
                 658 
               
               
                   
               
               
                 826195 
                 448 
                 463 
                 GAGCTCTCGGTAGGAG 
                 94 
                 5376 
                 5391 
                 659 
               
               
                   
               
               
                 826196 
                 451 
                 466 
                 GAAGAGCTCTCGGTAG 
                 114 
                 5379 
                 5394 
                 660 
               
               
                   
               
               
                 826197 
                 453 
                 468 
                 TCGAAGAGCTCTCGGT 
                 41 
                 5381 
                 5396 
                 661 
               
               
                   
               
               
                 826198 
                 456 
                 471 
                 AACTCGAAGAGCTCTC 
                 30 
                 5384 
                 5399 
                 662 
               
               
                   
               
               
                 826199 
                 457 
                 472 
                 GAACTCGAAGAGCTCT 
                 95 
                 5385 
                 5400 
                 663 
               
               
                   
               
               
                 826200 
                 466 
                 481 
                 GTTGCAGAAGAACTCG 
                 27 
                 5394 
                 5409 
                 664 
               
               
                   
               
               
                 826201 
                 467 
                 482 
                 TGTTGCAGAAGAACTC 
                 44 
                 5395 
                 5410 
                 665 
               
               
                   
               
               
                 826213 
                 575 
                 590 
                 GCCAGTACATCATGCC 
                 34 
                 5503 
                 5518 
                 666 
               
               
                   
               
               
                 826214 
                 577 
                 592 
                 TTGCCAGTACATCATG 
                 58 
                 5505 
                 5520 
                 667 
               
               
                   
               
               
                 826215 
                 580 
                 595 
                 GAATTGCCAGTACATC 
                 46 
                 5508 
                 5523 
                 668 
               
               
                   
               
               
                 826216 
                 581 
                 596 
                 CGAATTGCCAGTACAT 
                 27 
                 5509 
                 5524 
                 669 
               
               
                   
               
               
                 826217 
                 582 
                 597 
                 CCGAATTGCCAGTACA 
                 26 
                 5510 
                 5525 
                 670 
               
               
                   
               
               
                 826218 
                 585 
                 600 
                 AGGCCGAATTGCCAGT 
                 102 
                 5513 
                 5528 
                 671 
               
               
                   
               
               
                 826219 
                 587 
                 602 
                 GCAGGCCGAATTGCCA 
                 34 
                 5515 
                 5530 
                 672 
               
               
                   
               
               
                 826220 
                 589 
                 604 
                 AAGCAGGCCGAATTGC 
                 51 
                 5517 
                 5532 
                 673 
               
               
                   
               
               
                 826233 
                 626 
                 641 
                 TGTTGAGGCTGACGGG 
                 14 
                 5554 
                 5569 
                 674 
               
               
                   
               
               
                 826234 
                 628 
                 643 
                 GATGTTGAGGCTGACG 
                 28 
                 5556 
                 5571 
                 675 
               
               
                   
               
               
                 826235 
                 639 
                 654 
                 GAGTTGAGGTTGATGT 
                 64 
                 5567 
                 5582 
                 676 
               
               
                   
               
               
                 826236 
                 641 
                 656 
                 CCGAGTTGAGGTTGAT 
                 32 
                 5569 
                 5584 
                 677 
               
               
                   
               
               
                 826237 
                 643 
                 658 
                 GTCCGAGTTGAGGTTG 
                 28 
                 5571 
                 5586 
                 678 
               
               
                   
               
               
                 826238 
                 644 
                 659 
                 TGTCCGAGTTGAGGTT 
                 65 
                 5572 
                 5587 
                 679 
               
               
                   
               
               
                 826239 
                 645 
                 660 
                 TTGTCCGAGTTGAGGT 
                 40 
                 5573 
                 5588 
                 680 
               
               
                   
               
               
                 826240 
                 647 
                 662 
                 GCTTGTCCGAGTTGAG 
                 19 
                 5575 
                 5590 
                 681 
               
               
                   
               
               
                 826253 
                 731 
                 746 
                 TGCGGTCCAGCTCCTC 
                 42 
                 16316 
                 16331 
                 682 
               
               
                   
               
               
                 826254 
                 734 
                 749 
                 TGATGCGGTCCAGCTC 
                 88 
                 16319 
                 16334 
                 683 
               
               
                   
               
               
                 826255 
                 737 
                 752 
                 CTGTGATGCGGTCCAG 
                 38 
                 16322 
                 16337 
                 684 
               
               
                   
               
               
                 826256 
                 739 
                 754 
                 CTCTGTGATGCGGTCC 
                 20 
                 16324 
                 16339 
                 685 
               
               
                   
               
               
                 826257 
                 740 
                 755 
                 GCTCTGTGATGCGGTC 
                 43 
                 16325 
                 16340 
                 686 
               
               
                   
               
               
                 826258 
                 759 
                 774 
                 TACAGGTCAAAGAGCG 
                 52 
                 16344 
                 16359 
                 687 
               
               
                   
               
               
                 826259 
                 761 
                 776 
                 TGTACAGGTCAAAGAG 
                 18 
                 16346 
                 16361 
                 688 
               
               
                   
               
               
                 826260 
                 762 
                 777 
                 TTGTACAGGTCAAAGA 
                 41 
                 16347 
                 16362 
                 689 
               
               
                   
               
               
                 826271 
                 798 
                 813 
                 CGGGAGCCGGCCACGA 
                 55 
                 16383 
                 16398 
                 690 
               
               
                   
               
               
                 826272 
                 800 
                 815 
                 TGCGGGAGCCGGCCAC 
                 23 
                 16385 
                 16400 
                 691 
               
               
                   
               
               
                 826273 
                 803 
                 818 
                 GGCTGCGGGAGCCGGC 
                 147 
                 16388 
                 16403 
                 692 
               
               
                   
               
               
                 826274 
                 804 
                 819 
                 CGGCTGCGGGAGCCGG 
                 70 
                 16389 
                 16404 
                 693 
               
               
                   
               
               
                 826275 
                 805 
                 820 
                 ACGGCTGCGGGAGCCG 
                 104 
                 16390 
                 16405 
                 694 
               
               
                   
               
               
                 826276 
                 807 
                 822 
                 CGACGGCTGCGGGAGC 
                 101 
                 16392 
                 16407 
                 695 
               
               
                   
               
               
                 826277 
                 808 
                 823 
                 GCGACGGCTGCGGGAG 
                 52 
                 16393 
                 16408 
                 696 
               
               
                   
               
               
                 826278 
                 810 
                 825 
                 TCGCGACGGCTGCGGG 
                 52 
                 16395 
                 16410 
                 697 
               
               
                   
               
               
                 826291 
                 834 
                 849 
                 GGGTGCGGCAGAGTCC 
                 46 
                 16419 
                 16434 
                 698 
               
               
                   
               
               
                 826292 
                 858 
                 873 
                 GGCGGGACCCTCAGGC 
                 37 
                 16443 
                 16458 
                 699 
               
               
                   
               
               
                 826293 
                 877 
                 892 
                 ACGGGCCCCGTGAGGC 
                 85 
                 16462 
                 16477 
                 700 
               
               
                   
               
               
                 826294 
                 879 
                 894 
                 CGACGGGCCCCGTGAG 
                 49 
                 16464 
                 16479 
                 701 
               
               
                   
               
               
                 826295 
                 882 
                 897 
                 GCTCGACGGGCCCCGT 
                 27 
                 16467 
                 16482 
                 702 
               
               
                   
               
               
                 826296 
                 883 
                 898 
                 GGCTCGACGGGCCCCG 
                 64 
                 16468 
                 16483 
                 703 
               
               
                   
               
               
                 826297 
                 889 
                 904 
                 GCTACGGGCTCGACGG 
                 33 
                 16474 
                 16489 
                 704 
               
               
                   
               
               
                 826298 
                 891 
                 906 
                 ACGCTACGGGCTCGAC 
                 35 
                 16476 
                 16491 
                 705 
               
               
                   
               
               
                 826311 
                 952 
                 967 
                 GAAGCCGATCTTCCAG 
                 43 
                 16537 
                 16552 
                 706 
               
               
                   
               
               
                 826312 
                 953 
                 968 
                 GGAAGCCGATCTTCCA 
                 64 
                 16538 
                 16553 
                 707 
               
               
                   
               
               
                 826313 
                 954 
                 969 
                 TGGAAGCCGATCTTCC 
                 161 
                 16539 
                 16554 
                 708 
               
               
                   
               
               
                 826314 
                 956 
                 971 
                 GCTGGAAGCCGATCTT 
                 80 
                 16541 
                 16556 
                 709 
               
               
                   
               
               
                 826315 
                 958 
                 973 
                 CAGCTGGAAGCCGATC 
                 41 
                 16543 
                 16558 
                 710 
               
               
                   
               
               
                 826316 
                 968 
                 983 
                 TCTGGTTGCACAGCTG 
                 38 
                 N/A 
                 N/A 
                 711 
               
               
                   
               
               
                 826317 
                 969 
                 984 
                 TTCTGGTTGCACAGCT 
                 53 
                 N/A 
                 N/A 
                 712 
               
               
                   
               
               
                 826318 
                 970 
                 985 
                 GTTCTGGTTGCACAGC 
                 19 
                 N/A 
                 N/A 
                 713 
               
               
                   
               
               
                 826329 
                 983 
                 998 
                 AGCAGTCCGATTTGTT 
                 45 
                 17769 
                 17784 
                 714 
               
               
                   
               
               
                 826330 
                 985 
                 1000 
                 GAAGCAGTCCGATTTG 
                 72 
                 17771 
                 17786 
                 715 
               
               
                   
               
               
                 826331 
                 986 
                 1001 
                 AGAAGCAGTCCGATTT 
                 92 
                 17772 
                 17787 
                 716 
               
               
                   
               
               
                 826332 
                 987 
                 1002 
                 TAGAAGCAGTCCGATT 
                 124 
                 17773 
                 17788 
                 717 
               
               
                   
               
               
                 826333 
                 988 
                 1003 
                 GTAGAAGCAGTCCGAT 
                 50 
                 17774 
                 17789 
                 718 
               
               
                   
               
               
                 826334 
                 989 
                 1004 
                 GGTAGAAGCAGTCCGA 
                 18 
                 17775 
                 17790 
                 719 
               
               
                   
               
               
                 826335 
                 994 
                 1009 
                 TGTCTGGTAGAAGCAG 
                 25 
                 17780 
                 17795 
                 720 
               
               
                   
               
               
                 826336 
                 995 
                 1010 
                 ATGTCTGGTAGAAGCA 
                 35 
                 17781 
                 17796 
                 721 
               
               
                   
               
               
                 826349 
                 1022 
                 1037 
                 CCCTCACCGCATCCAC 
                 32 
                 17808 
                 17823 
                 722 
               
               
                   
               
               
                 826350 
                 1025 
                 1040 
                 ACTCCCTCACCGCATC 
                 68 
                 17811 
                 17826 
                 723 
               
               
                   
               
               
                 826351 
                 1026 
                 1041 
                 CACTCCCTCACCGCAT 
                 128 
                 17812 
                 17827 
                 724 
               
               
                   
               
               
                 826352 
                 1028 
                 1043 
                 ACCACTCCCTCACCGC 
                 33 
                 17814 
                 17829 
                 725 
               
               
                   
               
               
                 826353 
                 1032 
                 1047 
                 CGGTACCACTCCCTCA 
                 49 
                 17818 
                 17833 
                 726 
               
               
                   
               
               
                 826354 
                 1033 
                 1048 
                 GCGGTACCACTCCCTC 
                 69 
                 17819 
                 17834 
                 727 
               
               
                   
               
               
                 826355 
                 1034 
                 1049 
                 AGCGGTACCACTCCCT 
                 24 
                 17820 
                 17835 
                 728 
               
               
                   
               
               
                 826356 
                 1045 
                 1060 
                 GATGTAGTGGAAGCGG 
                 36 
                 17831 
                 17846 
                 729 
               
               
                   
               
               
                 826369 
                 1123 
                 1138 
                 GCGGCAGGCGAAGATG 
                 49 
                 17909 
                 17924 
                 730 
               
               
                   
               
               
                 826370 
                 1126 
                 1141 
                 GAAGCGGCAGGCGAAG 
                 55 
                 17912 
                 17927 
                 731 
               
               
                   
               
               
                 826371 
                 1129 
                 1144 
                 GTTGAAGCGGCAGGCG 
                 97 
                 17915 
                 17930 
                 732 
               
               
                   
               
               
                 826372 
                 1130 
                 1145 
                 GGTTGAAGCGGCAGGC 
                 30 
                 17916 
                 17931 
                 733 
               
               
                   
               
               
                 826373 
                 1134 
                 1149 
                 ACCTGGTTGAAGCGGC 
                 20 
                 17920 
                 17935 
                 734 
               
               
                   
               
               
                 826374 
                 1136 
                 1151 
                 AGACCTGGTTGAAGCG 
                 44 
                 17922 
                 17937 
                 735 
               
               
                   
               
               
                 826375 
                 1138 
                 1153 
                 GGAGACCTGGTTGAAG 
                 68 
                 17924 
                 17939 
                 736 
               
               
                   
               
               
                 826376 
                 1146 
                 1161 
                 TGGTTGCAGGAGACCT 
                 143 
                 17932 
                 17947 
                 737 
               
               
                   
               
               
                 826389 
                 1232 
                 1247 
                 AAGACATCCAGAGGTT 
                 80 
                 24188 
                 24203 
                 738 
               
               
                   
               
               
                 826390 
                 1250 
                 1265 
                 TGTTGATTCCAGGCAT 
                 53 
                 24206 
                 24221 
                 739 
               
               
                   
               
               
                 826391 
                 1251 
                 1266 
                 TTGTTGATTCCAGGCA 
                 43 
                 24207 
                 24222 
                 740 
               
               
                   
               
               
                 826392 
                 1252 
                 1267 
                 GTTGTTGATTCCAGGC 
                 31 
                 24208 
                 24223 
                 741 
               
               
                   
               
               
                 826393 
                 1254 
                 1269 
                 CCGTTGTTGATTCCAG 
                 15 
                 24210 
                 24225 
                 742 
               
               
                   
               
               
                 826394 
                 1255 
                 1270 
                 ACCGTTGTTGATTCCA 
                 15 
                 24211 
                 24226 
                 743 
               
               
                   
               
               
                 826395 
                 1257 
                 1272 
                 AGACCGTTGTTGATTC 
                 30 
                 N/A 
                 N/A 
                 744 
               
               
                   
               
               
                 826396 
                 1259 
                 1274 
                 ACAGACCGTTGTTGAT 
                 33 
                 N/A 
                 N/A 
                 745 
               
               
                   
               
               
                 826409 
                 1285 
                 1300 
                 ATTCTGCTCTGCGCGC 
                 39 
                 24582 
                 24597 
                 746 
               
               
                   
               
               
                 826410 
                 1286 
                 1301 
                 CATTCTGCTCTGCGCG 
                 36 
                 24583 
                 24598 
                 747 
               
               
                   
               
               
                 826411 
                 1287 
                 1302 
                 TCATTCTGCTCTGCGC 
                 80 
                 24584 
                 24599 
                 748 
               
               
                   
               
               
                 826412 
                 1323 
                 1338 
                 CGGGCCCCAGTCACTG 
                 118 
                 24620 
                 24635 
                 749 
               
               
                   
               
               
                 826413 
                 1325 
                 1340 
                 CCCGGGCCCCAGTCAC 
                 82 
                 24622 
                 24637 
                 750 
               
               
                   
               
               
                 826414 
                 1327 
                 1342 
                 TACCCGGGCCCCAGTC 
                 31 
                 24624 
                 24639 
                 751 
               
               
                   
               
               
                 826415 
                 1329 
                 1344 
                 ATTACCCGGGCCCCAG 
                 56 
                 24626 
                 24641 
                 752 
               
               
                   
               
               
                 826416 
                 1331 
                 1346 
                 CCATTACCCGGGCCCC 
                 37 
                 24628 
                 24643 
                 753 
               
               
                   
               
               
                 826429 
                 1366 
                 1381 
                 ATCATCCATAAAGGCA 
                 75 
                 24663 
                 24678 
                 754 
               
               
                   
               
               
                 826430 
                 1379 
                 1394 
                 AGTTAAAGCCACCATC 
                 57 
                 24676 
                 24691 
                 755 
               
               
                   
               
               
                 826431 
                 1383 
                 1398 
                 CGCAAGTTAAAGCCAC 
                 70 
                 24680 
                 24695 
                 756 
               
               
                   
               
               
                 826432 
                 1385 
                 1400 
                 GCCGCAAGTTAAAGCC 
                 33 
                 24682 
                 24697 
                 757 
               
               
                   
               
               
                 826433 
                 1387 
                 1402 
                 AGGCCGCAAGTTAAAG 
                 32 
                 24684 
                 24699 
                 758 
               
               
                   
               
               
                 826434 
                 1388 
                 1403 
                 CAGGCCGCAAGTTAAA 
                 47 
                 24685 
                 24700 
                 759 
               
               
                   
               
               
                 826435 
                 1389 
                 1404 
                 CCAGGCCGCAAGTTAA 
                 40 
                 24686 
                 24701 
                 760 
               
               
                   
               
               
                 826436 
                 1390 
                 1405 
                 GCCAGGCCGCAAGTTA 
                 40 
                 24687 
                 24702 
                 761 
               
               
                   
               
               
                 826449 
                 1446 
                 1461 
                 TAATCGCCCCCAAGTC 
                 35 
                 25166 
                 25181 
                 762 
               
               
                   
               
               
                 826450 
                 1447 
                 1462 
                 ATAATCGCCCCCAAGT 
                 56 
                 25167 
                 25182 
                 763 
               
               
                   
               
               
                 826451 
                 1448 
                 1463 
                 CATAATCGCCCCCAAG 
                 53 
                 25168 
                 25183 
                 764 
               
               
                   
               
               
                 826452 
                 1450 
                 1465 
                 GCCATAATCGCCCCCA 
                 23 
                 25170 
                 25185 
                 765 
               
               
                   
               
               
                 826453 
                 1451 
                 1466 
                 CGCCATAATCGCCCCC 
                 22 
                 25171 
                 25186 
                 766 
               
               
                   
               
               
                 826454 
                 1453 
                 1468 
                 GTCGCCATAATCGCCC 
                 43 
                 25173 
                 25188 
                 767 
               
               
                   
               
               
                 826455 
                 1457 
                 1472 
                 TGCAGTCGCCATAATC 
                 36 
                 25177 
                 25192 
                 768 
               
               
                   
               
               
                 826456 
                 1458 
                 1473 
                 GTGCAGTCGCCATAAT 
                 38 
                 25178 
                 25193 
                 769 
               
               
                   
               
               
                 826469 
                 1528 
                 1543 
                 GTGAATACACACCTGC 
                 98 
                 N/A 
                 N/A 
                 770 
               
               
                   
               
               
                 826470 
                 1530 
                 1545 
                 GAGTGAATACACACCT 
                 47 
                 25444 
                 25459 
                 771 
               
               
                   
               
               
                 826471 
                 1531 
                 1546 
                 GGAGTGAATACACACC 
                 128 
                 25445 
                 25460 
                 772 
               
               
                   
               
               
                 826472 
                 1534 
                 1549 
                 GCAGGAGTGAATACAC 
                 106 
                 25448 
                 25463 
                 773 
               
               
                   
               
               
                 826473 
                 1553 
                 1568 
                 TGATCATGCTCTCCTG 
                 25 
                 25467 
                 25482 
                 774 
               
               
                   
               
               
                 826474 
                 1554 
                 1569 
                 TTGATCATGCTCTCCT 
                 31 
                 25468 
                 25483 
                 775 
               
               
                   
               
               
                 826475 
                 1556 
                 1571 
                 CCTTGATCATGCTCTC 
                 28 
                 25470 
                 25485 
                 776 
               
               
                   
               
               
                 826476 
                 1557 
                 1572 
                 TCCTTGATCATGCTCT 
                 23 
                 25471 
                 25486 
                 777 
               
               
                   
               
               
                 826488 
                 1583 
                 1598 
                 GATAGAAGATGTAGGC 
                 38 
                 25497 
                 25512 
                 778 
               
               
                   
               
               
                 826489 
                 1584 
                 1599 
                 GGATAGAAGATGTAGG 
                 36 
                 25498 
                 25513 
                 779 
               
               
                   
               
               
                 826490 
                 1585 
                 1600 
                 CGGATAGAAGATGTAG 
                 101 
                 25499 
                 25514 
                 780 
               
               
                   
               
               
                 826491 
                 1587 
                 1602 
                 CGCGGATAGAAGATGT 
                 33 
                 25501 
                 25516 
                 781 
               
               
                   
               
               
                 826492 
                 1588 
                 1603 
                 CCGCGGATAGAAGATG 
                 69 
                 25502 
                 25517 
                 782 
               
               
                   
               
               
                 826493 
                 1589 
                 1604 
                 GCCGCGGATAGAAGAT 
                 64 
                 25503 
                 25518 
                 783 
               
               
                   
               
               
                 826494 
                 1591 
                 1606 
                 GGGCCGCGGATAGAAG 
                 48 
                 25505 
                 25520 
                 784 
               
               
                   
               
               
                 826495 
                 1612 
                 1627 
                 GTCACAGTACTCCACG 
                 27 
                 25526 
                 25541 
                 785 
               
               
                   
               
               
                 826508 
                 1669 
                 1684 
                 GGAGAAGTCAACCTGG 
                 21 
                 30615 
                 30630 
                 786 
               
               
                   
               
               
                 826509 
                 1675 
                 1690 
                 GTCTGAGGAGAAGTCA 
                 43 
                 30621 
                 30636 
                 787 
               
               
                   
               
               
                 826510 
                 1696 
                 1711 
                 CTTGGTGAAACAGCCC 
                 159 
                 30642 
                 30657 
                 788 
               
               
                   
               
               
                 826511 
                 1702 
                 1717 
                 CCGGCACTTGGTGAAA 
                 123 
                 30648 
                 30663 
                 789 
               
               
                   
               
               
                 826512 
                 1708 
                 1723 
                 TGGCTTCCGGCACTTG 
                 36 
                 30654 
                 30669 
                 790 
               
               
                   
               
               
                 826513 
                 1709 
                 1724 
                 ATGGCTTCCGGCACTT 
                 26 
                 30655 
                 30670 
                 791 
               
               
                   
               
               
                 826514 
                 1711 
                 1726 
                 GCATGGCTTCCGGCAC 
                 15 
                 30657 
                 30672 
                 792 
               
               
                   
               
               
                 826515 
                 1716 
                 1731 
                 ACGCTGCATGGCTTCC 
                 44 
                 N/A 
                 N/A 
                 793 
               
               
                   
               
               
                 826555 
                 1876 
                 1891 
                 TTTGTAGTTCAGCTCC 
                 67 
                 31232 
                 31247 
                 794 
               
               
                   
               
               
                 826568 
                 2001 
                 2016 
                 AGGTCAAAGACGAGCT 
                 76 
                 31830 
                 31845 
                 795 
               
               
                   
               
               
                 826569 
                 2002 
                 2017 
                 CAGGTCAAAGACGAGC 
                 28 
                 31831 
                 31846 
                 796 
               
               
                   
               
               
                 826570 
                 2003 
                 2018 
                 GCAGGTCAAAGACGAG 
                 103 
                 31832 
                 31847 
                 797 
               
               
                   
               
               
                 826571 
                 2009 
                 2024 
                 TGACCAGCAGGTCAAA 
                 148 
                 31838 
                 31853 
                 798 
               
               
                   
               
               
                 826572 
                 2011 
                 2026 
                 GATGACCAGCAGGTCA 
                 170 
                 31840 
                 31855 
                 799 
               
               
                   
               
               
                 826573 
                 2032 
                 2047 
                 TCGGAGCAGCATGAGG 
                 61 
                 31861 
                 31876 
                 800 
               
               
                   
               
               
                 826574 
                 2034 
                 2049 
                 CTTCGGAGCAGCATGA 
                 52 
                 31863 
                 31878 
                 801 
               
               
                   
               
               
                 826575 
                 2035 
                 2050 
                 CCTTCGGAGCAGCATG 
                 44 
                 31864 
                 31879 
                 802 
               
               
                   
               
               
                 826587 
                 2049 
                 2064 
                 TATCGGCTTCGGAACC 
                 28 
                 31878 
                 31893 
                 803 
               
               
                   
               
               
                 826588 
                 2050 
                 2065 
                 GTATCGGCTTCGGAAC 
                 50 
                 31879 
                 31894 
                 804 
               
               
                   
               
               
                 826589 
                 2051 
                 2066 
                 AGTATCGGCTTCGGAA 
                 28 
                 31880 
                 31895 
                 805 
               
               
                   
               
               
                 826590 
                 2053 
                 2068 
                 CCAGTATCGGCTTCGG 
                 37 
                 31882 
                 31897 
                 806 
               
               
                   
               
               
                 826591 
                 2054 
                 2069 
                 ACCAGTATCGGCTTCG 
                 23 
                 31883 
                 31898 
                 807 
               
               
                   
               
               
                 826592 
                 2055 
                 2070 
                 GACCAGTATCGGCTTC 
                 32 
                 31884 
                 31899 
                 808 
               
               
                   
               
               
                 826593 
                 2056 
                 2071 
                 AGACCAGTATCGGCTT 
                 20 
                 31885 
                 31900 
                 809 
               
               
                   
               
               
                 826594 
                 2058 
                 2073 
                 GGAGACCAGTATCGGC 
                 25 
                 31887 
                 31902 
                 810 
               
               
                   
               
               
                 826607 
                 2282 
                 2297 
                 AGGGCCCCCCCAGAGG 
                 90 
                 32111 
                 32126 
                 811 
               
               
                   
               
               
                 826608 
                 2284 
                 2299 
                 TCAGGGCCCCCCCAGA 
                 55 
                 32113 
                 32128 
                 812 
               
               
                   
               
               
                 826609 
                 2308 
                 2323 
                 GTGTGAGAAACCTCTC 
                 64 
                 32137 
                 32152 
                 813 
               
               
                   
               
               
                 826610 
                 2310 
                 2325 
                 TGGTGTGAGAAACCTC 
                 81 
                 32139 
                 32154 
                 814 
               
               
                   
               
               
                 826611 
                 2313 
                 2328 
                 CCTTGGTGTGAGAAAC 
                 93 
                 32142 
                 32157 
                 815 
               
               
                   
               
               
                 826612 
                 2314 
                 2329 
                 GCCTTGGTGTGAGAAA 
                 52 
                 32143 
                 32158 
                 816 
               
               
                   
               
               
                 826613 
                 2315 
                 2330 
                 TGCCTTGGTGTGAGAA 
                 31 
                 32144 
                 32159 
                 817 
               
               
                   
               
               
                 826614 
                 2316 
                 2331 
                 CTGCCTTGGTGTGAGA 
                 30 
                 32145 
                 32160 
                 818 
               
               
                   
               
               
                 826627 
                 2399 
                 2414 
                 ACGGCAGTTTGGGCGG 
                 34 
                 32228 
                 32243 
                 819 
               
               
                   
               
               
                 826628 
                 2400 
                 2415 
                 AACGGCAGTTTGGGCG 
                 93 
                 32229 
                 32244 
                 820 
               
               
                   
               
               
                 826629 
                 2401 
                 2416 
                 CAACGGCAGTTTGGGC 
                 87 
                 32230 
                 32245 
                 821 
               
               
                   
               
               
                 826630 
                 2403 
                 2418 
                 ATCAACGGCAGTTTGG 
                 60 
                 32232 
                 32247 
                 822 
               
               
                   
               
               
                 826631 
                 2405 
                 2420 
                 ACATCAACGGCAGTTT 
                 25 
                 32234 
                 32249 
                 823 
               
               
                   
               
               
                 826632 
                 2407 
                 2422 
                 ACACATCAACGGCAGT 
                 19 
                 32236 
                 32251 
                 824 
               
               
                   
               
               
                 826633 
                 2408 
                 2423 
                 CACACATCAACGGCAG 
                 38 
                 32237 
                 32252 
                 825 
               
               
                   
               
               
                 826634 
                 2410 
                 2425 
                 TCCACACATCAACGGC 
                 49 
                 32239 
                 32254 
                 826 
               
               
                   
               
               
                 826647 
                 2491 
                 2506 
                 GGAGCCAAGGCACTTC 
                 30 
                 32320 
                 32335 
                 827 
               
               
                   
               
               
                 826648 
                 2492 
                 2507 
                 TGGAGCCAAGGCACTT 
                 31 
                 32321 
                 32336 
                 828 
               
               
                   
               
               
                 826649 
                 2502 
                 2517 
                 GGTACAGGGCTGGAGC 
                 117 
                 32331 
                 32346 
                 829 
               
               
                   
               
               
                 826650 
                 2520 
                 2535 
                 TCAGAGGCAGTACCAA 
                 48 
                 32349 
                 32364 
                 830 
               
               
                   
               
               
                 826651 
                 2523 
                 2538 
                 TGTTCAGAGGCAGTAC 
                 38 
                 32352 
                 32367 
                 831 
               
               
                   
               
               
                 826652 
                 2533 
                 2548 
                 GAAACCAGAGTGTTCA 
                 52 
                 32362 
                 32377 
                 832 
               
               
                   
               
               
                 826653 
                 2551 
                 2566 
                 TAGCCGCAGTTGGGTG 
                 49 
                 32380 
                 32395 
                 833 
               
               
                   
               
               
                 826654 
                 2552 
                 2567 
                 TTAGCCGCAGTTGGGT 
                 28 
                 32381 
                 32396 
                 834 
               
               
                   
               
               
                 826665 
                 2579 
                 2594 
                 CTTGGCTGATCCAAGG 
                 33 
                 32408 
                 32423 
                 835 
               
               
                   
               
               
                 826666 
                 2580 
                 2595 
                 GCTTGGCTGATCCAAG 
                 69 
                 32409 
                 32424 
                 836 
               
               
                   
               
               
                 826667 
                 2581 
                 2596 
                 CGCTTGGCTGATCCAA 
                 66 
                 32410 
                 32425 
                 837 
               
               
                   
               
               
                 826668 
                 2582 
                 2597 
                 TCGCTTGGCTGATCCA 
                 10 
                 32411 
                 32426 
                 838 
               
               
                   
               
               
                 826669 
                 2583 
                 2598 
                 TTCGCTTGGCTGATCC 
                 33 
                 32412 
                 32427 
                 839 
               
               
                   
               
               
                 826670 
                 2584 
                 2599 
                 TTTCGCTTGGCTGATC 
                 43 
                 32413 
                 32428 
                 840 
               
               
                   
               
               
                 826671 
                 2585 
                 2600 
                 GTTTCGCTTGGCTGAT 
                 24 
                 32414 
                 32429 
                 841 
               
               
                   
               
               
                 826672 
                 2586 
                 2601 
                 AGTTTCGCTTGGCTGA 
                 39 
                 32415 
                 32430 
                 842 
               
               
                   
               
               
                 826685 
                 2623 
                 2638 
                 CAGCGGTTTCTTAGGA 
                 27 
                 32452 
                 32467 
                 843 
               
               
                   
               
               
                 826686 
                 2625 
                 2640 
                 ATCAGCGGTTTCTTAG 
                 45 
                 32454 
                 32469 
                 844 
               
               
                   
               
               
                 826687 
                 2627 
                 2642 
                 TTATCAGCGGTTTCTT 
                 18 
                 32456 
                 32471 
                 845 
               
               
                   
               
               
                 826688 
                 2629 
                 2644 
                 GGTTATCAGCGGTTTC 
                 33 
                 32458 
                 32473 
                 846 
               
               
                   
               
               
                 826689 
                 2632 
                 2647 
                 CCTGGTTATCAGCGGT 
                 29 
                 32461 
                 32476 
                 847 
               
               
                   
               
               
                 826690 
                 2634 
                 2649 
                 GTCCTGGTTATCAGCG 
                 19 
                 32463 
                 32478 
                 848 
               
               
                   
               
               
                 826691 
                 2636 
                 2651 
                 TTGTCCTGGTTATCAG 
                 36 
                 32465 
                 32480 
                 849 
               
               
                   
               
               
                 826692 
                 2652 
                 2667 
                 TACCCTTGGTTGTGTT 
                 39 
                 32481 
                 32496 
                 850 
               
               
                   
               
               
                 826705 
                 2692 
                 2707 
                 TAAGCCGTCGCTGGGC 
                 51 
                 32521 
                 32536 
                 851 
               
               
                   
               
               
                 826706 
                 2693 
                 2708 
                 TTAAGCCGTCGCTGGG 
                 63 
                 32522 
                 32537 
                 852 
               
               
                   
               
               
                 826707 
                 2696 
                 2711 
                 GGCTTAAGCCGTCGCT 
                 58 
                 32525 
                 32540 
                 853 
               
               
                   
               
               
                 826708 
                 2698 
                 2713 
                 CTGGCTTAAGCCGTCG 
                 31 
                 32527 
                 32542 
                 854 
               
               
                   
               
               
                 826709 
                 2700 
                 2715 
                 GGCTGGCTTAAGCCGT 
                 131 
                 32529 
                 32544 
                 855 
               
               
                   
               
               
                 826710 
                 2701 
                 2716 
                 GGGCTGGCTTAAGCCG 
                 92 
                 32530 
                 32545 
                 856 
               
               
                   
               
               
                 826711 
                 2734 
                 2749 
                 GCTACTGGAGAGCAGT 
                 20 
                 32563 
                 32578 
                 857 
               
               
                   
               
               
                 826712 
                 2735 
                 2750 
                 TGCTACTGGAGAGCAG 
                 53 
                 32564 
                 32579 
                 858 
               
               
                   
               
               
                 826725 
                 2846 
                 2861 
                 TCTGCTCTAGCCCTAG 
                 53 
                 32675 
                 32690 
                 859 
               
               
                   
               
               
                 826726 
                 2847 
                 2862 
                 GTCTGCTCTAGCCCTA 
                 32 
                 32676 
                 32691 
                 860 
               
               
                   
               
               
                 826727 
                 2850 
                 2865 
                 CGGGTCTGCTCTAGCC 
                 107 
                 32679 
                 32694 
                 861 
               
               
                   
               
               
                 826728 
                 2852 
                 2867 
                 CCCGGGTCTGCTCTAG 
                 66 
                 32681 
                 32696 
                 862 
               
               
                   
               
               
                 826729 
                 2854 
                 2869 
                 TACCCGGGTCTGCTCT 
                 92 
                 32683 
                 32698 
                 863 
               
               
                   
               
               
                 826730 
                 2855 
                 2870 
                 TTACCCGGGTCTGCTC 
                 52 
                 32684 
                 32699 
                 864 
               
               
                   
               
               
                 826731 
                 2856 
                 2871 
                 CTTACCCGGGTCTGCT 
                 51 
                 32685 
                 32700 
                 865 
               
               
                   
               
               
                 826732 
                 2858 
                 2873 
                 TACTTACCCGGGTCTG 
                 38 
                 32687 
                 32702 
                 866 
               
               
                   
               
               
                 826745 
                 2954 
                 2969 
                 AGACATGTAGAGGTAT 
                 16 
                 32783 
                 32798 
                 867 
               
               
                   
               
               
                 826746 
                 2955 
                 2970 
                 CAGACATGTAGAGGTA 
                 8 
                 32784 
                 32799 
                 868 
               
               
                   
               
               
                 826747 
                 2959 
                 2974 
                 CAAGCAGACATGTAGA 
                 32 
                 32788 
                 32803 
                 869 
               
               
                   
               
               
                 826748 
                 2960 
                 2975 
                 TCAAGCAGACATGTAG 
                 25 
                 32789 
                 32804 
                 870 
               
               
                   
               
               
                 826749 
                 2961 
                 2976 
                 CTCAAGCAGACATGTA 
                 22 
                 32790 
                 32805 
                 871 
               
               
                   
               
               
                 826750 
                 2963 
                 2978 
                 ATCTCAAGCAGACATG 
                 50 
                 32792 
                 32807 
                 872 
               
               
                   
               
               
                 826751 
                 2964 
                 2979 
                 TATCTCAAGCAGACAT 
                 26 
                 32793 
                 32808 
                 873 
               
               
                   
               
               
                 826752 
                 2965 
                 2980 
                 ATATCTCAAGCAGACA 
                 27 
                 32794 
                 32809 
                 874 
               
               
                   
               
               
                 826764 
                 3016 
                 3031 
                 ATGCATAGGAGTTCTC 
                 17 
                 32845 
                 32860 
                 875 
               
               
                   
               
               
                 826765 
                 3017 
                 3032 
                 GATGCATAGGAGTTCT 
                 23 
                 32846 
                 32861 
                 876 
               
               
                   
               
               
                 826766 
                 3019 
                 3034 
                 GGGATGCATAGGAGTT 
                 42 
                 32848 
                 32863 
                 877 
               
               
                   
               
               
                 826767 
                 3021 
                 3036 
                 AAGGGATGCATAGGAG 
                 61 
                 32850 
                 32865 
                 878 
               
               
                   
               
               
                 826768 
                 3022 
                 3037 
                 TAAGGGATGCATAGGA 
                 41 
                 32851 
                 32866 
                 879 
               
               
                   
               
               
                 826769 
                 3023 
                 3038 
                 CTAAGGGATGCATAGG 
                 37 
                 32852 
                 32867 
                 880 
               
               
                   
               
               
                 826770 
                 3024 
                 3039 
                 TCTAAGGGATGCATAG 
                 34 
                 32853 
                 32868 
                 881 
               
               
                   
               
               
                 826771 
                 3026 
                 3041 
                 GTTCTAAGGGATGCAT 
                 25 
                 32855 
                 32870 
                 882 
               
               
                   
               
               
                 826784 
                 3121 
                 3136 
                 TGTGCTACGGGAGCCC 
                 17 
                 32950 
                 32965 
                 883 
               
               
                   
               
               
                 826785 
                 3122 
                 3137 
                 GTGTGCTACGGGAGCC 
                 10 
                 32951 
                 32966 
                 884 
               
               
                   
               
               
                 826786 
                 3123 
                 3138 
                 AGTGTGCTACGGGAGC 
                 59 
                 32952 
                 32967 
                 885 
               
               
                   
               
               
                 826787 
                 3124 
                 3139 
                 TAGTGTGCTACGGGAG 
                 12 
                 32953 
                 32968 
                 886 
               
               
                   
               
               
                 826788 
                 3125 
                 3140 
                 ATAGTGTGCTACGGGA 
                 44 
                 32954 
                 32969 
                 887 
               
               
                   
               
               
                 826789 
                 3126 
                 3141 
                 TATAGTGTGCTACGGG 
                 46 
                 32955 
                 32970 
                 888 
               
               
                   
               
               
                 826790 
                 3128 
                 3143 
                 GTTATAGTGTGCTACG 
                 23 
                 32957 
                 32972 
                 889 
               
               
                   
               
               
                 826803 
                 3153 
                 3168 
                 GTGCAACAGCAACACT 
                 67 
                 32982 
                 32997 
                 890 
               
               
                   
               
               
                 826804 
                 3154 
                 3169 
                 GGTGCAACAGCAACAC 
                 37 
                 32983 
                 32998 
                 891 
               
               
                   
               
               
                 826805 
                 3155 
                 3170 
                 TGGTGCAACAGCAACA 
                 64 
                 32984 
                 32999 
                 892 
               
               
                   
               
               
                 826806 
                 3156 
                 3171 
                 ATGGTGCAACAGCAAC 
                 10 
                 32985 
                 33000 
                 893 
               
               
                   
               
               
                 826807 
                 3157 
                 3172 
                 TATGGTGCAACAGCAA 
                 20 
                 32986 
                 33001 
                 894 
               
               
                   
               
               
                 826808 
                 3158 
                 3173 
                 GTATGGTGCAACAGCA 
                 20 
                 32987 
                 33002 
                 895 
               
               
                   
               
               
                 826809 
                 3159 
                 3174 
                 AGTATGGTGCAACAGC 
                 4 
                 32988 
                 33003 
                 896 
               
               
                   
               
               
                 826821 
                 3216 
                 3231 
                 CCCTGACCGCAAGGCA 
                 16 
                 33045 
                 33060 
                 897 
               
               
                   
               
               
                 826822 
                 3217 
                 3232 
                 TCCCTGACCGCAAGGC 
                 51 
                 33046 
                 33061 
                 898 
               
               
                   
               
               
                 826823 
                 3218 
                 3233 
                 GTCCCTGACCGCAAGG 
                 35 
                 33047 
                 33062 
                 899 
               
               
                   
               
               
                 826824 
                 3219 
                 3234 
                 AGTCCCTGACCGCAAG 
                 34 
                 33048 
                 33063 
                 900 
               
               
                   
               
               
                 826825 
                 3220 
                 3235 
                 CAGTCCCTGACCGCAA 
                 33 
                 33049 
                 33064 
                 901 
               
               
                   
               
               
                 826826 
                 3222 
                 3237 
                 TTCAGTCCCTGACCGC 
                 23 
                 33051 
                 33066 
                 902 
               
               
                   
               
               
                 826827 
                 3223 
                 3238 
                 ATTCAGTCCCTGACCG 
                 69 
                 33052 
                 33067 
                 903 
               
               
                   
               
               
                 826828 
                 3225 
                 3240 
                 AGATTCAGTCCCTGAC 
                 14 
                 33054 
                 33069 
                 904 
               
               
                   
               
               
                 826840 
                 3239 
                 3254 
                 TACATAAACGGGCAAG 
                 50 
                 33068 
                 33083 
                 905 
               
               
                   
               
               
                 826841 
                 3242 
                 3257 
                 GCATACATAAACGGGC 
                 61 
                 33071 
                 33086 
                 906 
               
               
                   
               
               
                 826842 
                 3244 
                 3259 
                 GAGCATACATAAACGG 
                 59 
                 33073 
                 33088 
                 907 
               
               
                   
               
               
                 826843 
                 3249 
                 3264 
                 ACATGGAGCATACATA 
                 94 
                 33078 
                 33093 
                 908 
               
               
                   
               
               
                 826844 
                 3250 
                 3265 
                 GACATGGAGCATACAT 
                 42 
                 33079 
                 33094 
                 909 
               
               
                   
               
               
                 826845 
                 3251 
                 3266 
                 AGACATGGAGCATACA 
                 81 
                 33080 
                 33095 
                 910 
               
               
                   
               
               
                 826846 
                 3253 
                 3268 
                 CTAGACATGGAGCATA 
                 69 
                 33082 
                 33097 
                 911 
               
               
                   
               
               
                 826847 
                 3254 
                 3269 
                 GCTAGACATGGAGCAT 
                 47 
                 33083 
                 33098 
                 912 
               
               
                   
               
               
                 826860 
                 N/A 
                 N/A 
                 TGATACCTCCCCTTGG 
                 78 
                 2720 
                 2735 
                 913 
               
               
                   
               
               
                 826861 
                 N/A 
                 N/A 
                 ATGATACCTCCCCTTG 
                 84 
                 2721 
                 2736 
                 914 
               
               
                   
               
               
                 826862 
                 N/A 
                 N/A 
                 GCTCATGATACCTCCC 
                 176 
                 2725 
                 2740 
                 915 
               
               
                   
               
               
                 826863 
                 N/A 
                 N/A 
                 ACTGCTCATGATACCT 
                 95 
                 2728 
                 2743 
                 916 
               
               
                   
               
               
                 826864 
                 N/A 
                 N/A 
                 ATACTGCTCATGATAC 
                 59 
                 2730 
                 2745 
                 917 
               
               
                   
               
               
                 826865 
                 N/A 
                 N/A 
                 GATACTGCTCATGATA 
                 87 
                 2731 
                 2746 
                 918 
               
               
                   
               
               
                 826866 
                 N/A 
                 N/A 
                 CTTGATACTGCTCATG 
                 96 
                 2734 
                 2749 
                 919 
               
               
                   
               
               
                 826867 
                 N/A 
                 N/A 
                 CCTTGATACTGCTCAT 
                 79 
                 2735 
                 2750 
                 920 
               
               
                   
               
               
                 826880 
                 N/A 
                 N/A 
                 CGAGTTTTGTCCTAGC 
                 7 
                 4876 
                 4891 
                 921 
               
               
                   
               
               
                 826881 
                 N/A 
                 N/A 
                 TCGAGTTTTGTCCTAG 
                 30 
                 4877 
                 4892 
                 922 
               
               
                   
               
               
                 826882 
                 N/A 
                 N/A 
                 CTTTCGAGTTTTGTCC 
                 86 
                 4880 
                 4895 
                 923 
               
               
                   
               
               
                 826883 
                 N/A 
                 N/A 
                 CACCTTTCGAGTTTTG 
                 30 
                 4883 
                 4898 
                 924 
               
               
                   
               
               
                 826884 
                 N/A 
                 N/A 
                 GCCACCTTTCGAGTTT 
                 50 
                 4885 
                 4900 
                 925 
               
               
                   
               
               
                 826885 
                 N/A 
                 N/A 
                 GGGCCACCTTTCGAGT 
                 21 
                 4887 
                 4902 
                 926 
               
               
                   
               
               
                 826886 
                 N/A 
                 N/A 
                 TAGGGCCACCTTTCGA 
                 20 
                 4889 
                 4904 
                 927 
               
               
                   
               
               
                 826887 
                 N/A 
                 N/A 
                 ATAGGGCCACCTTTCG 
                 27 
                 4890 
                 4905 
                 928 
               
               
                   
               
               
                 826900 
                 N/A 
                 N/A 
                 GCCGGAGCTGGGCTTC 
                 55 
                 4935 
                 4950 
                 929 
               
               
                   
               
               
                 826901 
                 N/A 
                 N/A 
                 TGCCGGAGCTGGGCTT 
                 82 
                 4936 
                 4951 
                 930 
               
               
                   
               
               
                 826902 
                 N/A 
                 N/A 
                 GTGCCGGAGCTGGGCT 
                 179 
                 4937 
                 4952 
                 931 
               
               
                   
               
               
                 826903 
                 N/A 
                 N/A 
                 AAGTGCCGGAGCTGGG 
                 31 
                 4939 
                 4954 
                 932 
               
               
                   
               
               
                 826904 
                 N/A 
                 N/A 
                 AAAAGTGCCGGAGCTG 
                 44 
                 4941 
                 4956 
                 933 
               
               
                   
               
               
                 826905 
                 N/A 
                 N/A 
                 CCAAAAGTGCCGGAGC 
                 34 
                 4943 
                 4958 
                 934 
               
               
                   
               
               
                 826906 
                 N/A 
                 N/A 
                 GCCAAAAGTGCCGGAG 
                 19 
                 4944 
                 4959 
                 935 
               
               
                   
               
               
                 826907 
                 N/A 
                 N/A 
                 GGCCAAAAGTGCCGGA 
                 38 
                 4945 
                 4960 
                 936 
               
               
                   
               
               
                 826920 
                 N/A 
                 N/A 
                 CCCCTGGAACCCGAGT 
                 31 
                 5065 
                 5080 
                 937 
               
               
                   
               
               
                 826921 
                 N/A 
                 N/A 
                 CACCCCTGGAACCCGA 
                 38 
                 5067 
                 5082 
                 938 
               
               
                   
               
               
                 826922 
                 N/A 
                 N/A 
                 CCCGGAGTGGATTGGG 
                 185 
                 5138 
                 5153 
                 939 
               
               
                   
               
               
                 826923 
                 N/A 
                 N/A 
                 GCCCCGGAGTGGATTG 
                 76 
                 5140 
                 5155 
                 940 
               
               
                   
               
               
                 826924 
                 N/A 
                 N/A 
                 GAGCCCCGGAGTGGAT 
                 64 
                 5142 
                 5157 
                 941 
               
               
                   
               
               
                 826925 
                 N/A 
                 N/A 
                 ATGAGCCCCGGAGTGG 
                 28 
                 5144 
                 5159 
                 942 
               
               
                   
               
               
                 826926 
                 N/A 
                 N/A 
                 TTCATGAGCCCCGGAG 
                 42 
                 5147 
                 5162 
                 943 
               
               
                   
               
               
                 826927 
                 N/A 
                 N/A 
                 CCTTCATGAGCCCCGG 
                 29 
                 5149 
                 5164 
                 944 
               
               
                   
               
               
                 826940 
                 N/A 
                 N/A 
                 TGCTTACCTTGATACT 
                 152 
                 2741 
                 2756 
                 945 
               
               
                   
               
               
                 826941 
                 N/A 
                 N/A 
                 CCAAACCAGGTTCCCT 
                 104 
                 2757 
                 2772 
                 946 
               
               
                   
               
               
                 826942 
                 N/A 
                 N/A 
                 AGCCGGTGTCAACCAG 
                 187 
                 2777 
                 2792 
                 947 
               
               
                   
               
               
                 826943 
                 N/A 
                 N/A 
                 AAAGTGAAAGCCGGTG 
                 138 
                 2785 
                 2800 
                 948 
               
               
                   
               
               
                 826944 
                 N/A 
                 N/A 
                 TGCGACTTCTTAAAGT 
                 97 
                 2796 
                 2811 
                 949 
               
               
                   
               
               
                 826945 
                 N/A 
                 N/A 
                 GCTCAGGGTCCAACCT 
                 79 
                 2844 
                 2859 
                 950 
               
               
                   
               
               
                 826946 
                 N/A 
                 N/A 
                 AGCAAGGAGTTTAGCA 
                 95 
                 2889 
                 2904 
                 951 
               
               
                   
               
               
                 826947 
                 N/A 
                 N/A 
                 CATAAGAGCCAAGGGC 
                 142 
                 2983 
                 2998 
                 952 
               
               
                   
               
               
                 826960 
                 N/A 
                 N/A 
                 CGTTGATGGGCTATAT 
                 168 
                 3408 
                 3423 
                 953 
               
               
                   
               
               
                 826961 
                 N/A 
                 N/A 
                 CGCCTAGACAGGCCCT 
                 61 
                 3440 
                 3455 
                 954 
               
               
                   
               
               
                 826962 
                 N/A 
                 N/A 
                 ACGCAGGACACTGTGG 
                 90 
                 3555 
                 3570 
                 955 
               
               
                   
               
               
                 826963 
                 N/A 
                 N/A 
                 AGGCAGCGCGAGGGCC 
                 109 
                 3571 
                 3586 
                 956 
               
               
                   
               
               
                 826964 
                 N/A 
                 N/A 
                 GTGTAATCGCCCCTGC 
                 84 
                 3622 
                 3637 
                 957 
               
               
                   
               
               
                 826965 
                 N/A 
                 N/A 
                 GGCCCTAGGACATTCT 
                 73 
                 3674 
                 3689 
                 958 
               
               
                   
               
               
                 826966 
                 N/A 
                 N/A 
                 GTCCAGACCCGGGAGG 
                 66 
                 3718 
                 3733 
                 959 
               
               
                   
               
               
                 826967 
                 N/A 
                 N/A 
                 GGGAGCAGCGCACTCA 
                 106 
                 3804 
                 3819 
                 960 
               
               
                   
               
               
                 826980 
                 N/A 
                 N/A 
                 GGGACTAACCGACCTG 
                 146 
                 5631 
                 5646 
                 961 
               
               
                   
               
               
                 826981 
                 N/A 
                 N/A 
                 TTCCAGGCGCAGGCAC 
                 76 
                 5662 
                 5677 
                 962 
               
               
                   
               
               
                 826982 
                 N/A 
                 N/A 
                 CAGTAAGCTGGAGGCT 
                 181 
                 5785 
                 5800 
                 963 
               
               
                   
               
               
                 826983 
                 N/A 
                 N/A 
                 CGCCAGTCCAGTAAGC 
                 137 
                 5793 
                 5808 
                 964 
               
               
                   
               
               
                 826984 
                 N/A 
                 N/A 
                 GCTAGGATGGCTCCAC 
                 59 
                 5819 
                 5834 
                 965 
               
               
                   
               
               
                 826985 
                 N/A 
                 N/A 
                 CCACACTCTGGGTGAG 
                 42 
                 5843 
                 5858 
                 966 
               
               
                   
               
               
                 826986 
                 N/A 
                 N/A 
                 GGGCAATGCTGCTCCA 
                 76 
                 5863 
                 5878 
                 967 
               
               
                   
               
               
                 826987 
                 N/A 
                 N/A 
                 TCCCACTTGCAGGAGG 
                 91 
                 5919 
                 5934 
                 968 
               
               
                   
               
               
                 827000 
                 N/A 
                 N/A 
                 TCCCAAGGTGTGGCAT 
                 44 
                 6462 
                 6477 
                 969 
               
               
                   
               
               
                 827001 
                 N/A 
                 N/A 
                 TTGAAGCAGGTGTTCC 
                 60 
                 6475 
                 6490 
                 970 
               
               
                   
               
               
                 827002 
                 N/A 
                 N/A 
                 TGCCAGGTGCCTAGCC 
                 55 
                 6502 
                 6517 
                 971 
               
               
                   
               
               
                 827003 
                 N/A 
                 N/A 
                 CAATAAAGGGCTTATG 
                 94 
                 6538 
                 6553 
                 972 
               
               
                   
               
               
                 827004 
                 N/A 
                 N/A 
                 AACTACCTGGCCTTCA 
                 63 
                 6552 
                 6567 
                 973 
               
               
                   
               
               
                 827005 
                 N/A 
                 N/A 
                 GGCTTATATGCCTGTC 
                 77 
                 6605 
                 6620 
                 974 
               
               
                   
               
               
                 827006 
                 N/A 
                 N/A 
                 TGCCACAGTTACTGGC 
                 80 
                 6618 
                 6633 
                 975 
               
               
                   
               
               
                 827007 
                 N/A 
                 N/A 
                 ACTTATCCCAGTGTGC 
                 30 
                 6631 
                 6646 
                 976 
               
               
                   
               
               
                 827020 
                 N/A 
                 N/A 
                 AGGAAATGGTCCCTAC 
                 70 
                 6912 
                 6927 
                 977 
               
               
                   
               
               
                 827021 
                 N/A 
                 N/A 
                 GTGCACACGGCAGCTT 
                 77 
                 6932 
                 6947 
                 978 
               
               
                   
               
               
                 827022 
                 N/A 
                 N/A 
                 CCCAAGACACCTTCGC 
                 55 
                 6955 
                 6970 
                 979 
               
               
                   
               
               
                 827023 
                 N/A 
                 N/A 
                 TAGCACCGGGCTTGTA 
                 62 
                 6994 
                 7009 
                 980 
               
               
                   
               
               
                 827024 
                 N/A 
                 N/A 
                 AACAGGATGAGTCACA 
                 26 
                 7088 
                 7103 
                 981 
               
               
                   
               
               
                 827025 
                 N/A 
                 N/A 
                 AGTTTTGGGATTAGGC 
                 51 
                 7107 
                 7122 
                 982 
               
               
                   
               
               
                 827026 
                 N/A 
                 N/A 
                 GGCGGAAGCCACATCT 
                 61 
                 7178 
                 7193 
                 983 
               
               
                   
               
               
                 827027 
                 N/A 
                 N/A 
                 TGAAATGAGGCGGAAG 
                 117 
                 7186 
                 7201 
                 984 
               
               
                   
               
               
                 827040 
                 N/A 
                 N/A 
                 GGGAATAATACTGCCC 
                 115 
                 7751 
                 7766 
                 985 
               
               
                   
               
               
                 827041 
                 N/A 
                 N/A 
                 AATGTATGTTCCCTTG 
                 34 
                 7816 
                 7831 
                 986 
               
               
                   
               
               
                 827042 
                 N/A 
                 N/A 
                 GTAAAAAGTCTGGCCC 
                 34 
                 8222 
                 8237 
                 987 
               
               
                   
               
               
                 827043 
                 N/A 
                 N/A 
                 TCCAAGGTGTGTTGTG 
                 35 
                 8283 
                 8298 
                 988 
               
               
                   
               
               
                 827044 
                 N/A 
                 N/A 
                 CATGAGACCTACTTCC 
                 30 
                 8296 
                 8311 
                 989 
               
               
                   
               
               
                 827045 
                 N/A 
                 N/A 
                 ATAAGAGTCATCATGA 
                 54 
                 8307 
                 8322 
                 990 
               
               
                   
               
               
                 827046 
                 N/A 
                 N/A 
                 GGTGAGGGTGGACGGT 
                 86 
                 8444 
                 8459 
                 991 
               
               
                   
               
               
                 827047 
                 N/A 
                 N/A 
                 GGCTTTCCATTGGAGC 
                 64 
                 8483 
                 8498 
                 992 
               
               
                   
               
               
                 827060 
                 N/A 
                 N/A 
                 CCTCAGCAGGTAGGCA 
                 45 
                 8836 
                 8851 
                 993 
               
               
                   
               
               
                 827061 
                 N/A 
                 N/A 
                 TCGGACTCAGCACTTC 
                 75 
                 8961 
                 8976 
                 994 
               
               
                   
               
               
                 827062 
                 N/A 
                 N/A 
                 CTGCAGTGGCCAACCC 
                 98 
                 8983 
                 8998 
                 995 
               
               
                   
               
               
                 827063 
                 N/A 
                 N/A 
                 CTGTAGGTATGACTGG 
                 31 
                 9047 
                 9062 
                 996 
               
               
                   
               
               
                 827064 
                 N/A 
                 N/A 
                 TTCCATGACTGTAGGT 
                 24 
                 9055 
                 9070 
                 997 
               
               
                   
               
               
                 827065 
                 N/A 
                 N/A 
                 GCCTAAACCGTTCCTG 
                 53 
                 9105 
                 9120 
                 998 
               
               
                   
               
               
                 827066 
                 N/A 
                 N/A 
                 TTCAAGAACCCCAAGT 
                 50 
                 9132 
                 9147 
                 999 
               
               
                   
               
               
                 827067 
                 N/A 
                 N/A 
                 AGAAGCTACCATGACC 
                 66 
                 9158 
                 9173 
                 1000 
               
               
                   
               
               
                 827080 
                 N/A 
                 N/A 
                 GGCCTATCAACTAGGC 
                 154 
                 9783 
                 9798 
                 1001 
               
               
                   
               
               
                 827081 
                 N/A 
                 N/A 
                 CACAATTCCATCGGGC 
                 22 
                 9837 
                 9852 
                 1002 
               
               
                   
               
               
                 827082 
                 N/A 
                 N/A 
                 CCCTACATTGGAGGGT 
                 188 
                 9866 
                 9881 
                 1003 
               
               
                   
               
               
                 827083 
                 N/A 
                 N/A 
                 AGGGATAAAGAATGCC 
                 57 
                 9978 
                 9993 
                 1004 
               
               
                   
               
               
                 827084 
                 N/A 
                 N/A 
                 GACCAGCGGCTGGAGG 
                 46 
                 9996 
                 10011 
                 1005 
               
               
                   
               
               
                 827085 
                 N/A 
                 N/A 
                 AGACATCCGATCTTGT 
                 42 
                 10020 
                 10035 
                 1006 
               
               
                   
               
               
                 827086 
                 N/A 
                 N/A 
                 TGACACCTAGAGCTAA 
                 60 
                 10068 
                 10083 
                 1007 
               
               
                   
               
               
                 827087 
                 N/A 
                 N/A 
                 GGCAGAGCCTTTGAGT 
                 58 
                 10084 
                 10099 
                 1008 
               
               
                   
               
               
                 827100 
                 N/A 
                 N/A 
                 TACGCACCTCCCTCCT 
                 41 
                 10649 
                 10664 
                 1009 
               
               
                   
                   
                   
                   
                   
                 10672 
                 10687 
                   
               
               
                   
               
               
                 827101 
                 N/A 
                 N/A 
                 CTACGCACCTCCCTCC 
                 128 
                 10650 
                 10665 
                 1010 
               
               
                   
                   
                   
                   
                   
                 10673 
                 10688 
                   
               
               
                   
               
               
                 827102 
                 N/A 
                 N/A 
                 CCCTACGCACCTCCCT 
                 88 
                 10652 
                 10667 
                 1011 
               
               
                   
                   
                   
                   
                   
                 10675 
                 10690 
                   
               
               
                   
               
               
                 827103 
                 N/A 
                 N/A 
                 ACCCTACGCACCTCCC 
                 34 
                 10653 
                 10668 
                 1012 
               
               
                   
                   
                   
                   
                   
                 10676 
                 10691 
                   
               
               
                   
               
               
                 827104 
                 N/A 
                 N/A 
                 CCACCCTACGCACCTC 
                 36 
                 10655 
                 10670 
                 1013 
               
               
                   
                   
                   
                   
                   
                 10678 
                 10693 
                   
               
               
                   
               
               
                 827105 
                 N/A 
                 N/A 
                 GCCACCCTACGCACCT 
                 40 
                 10656 
                 10671 
                 1014 
               
               
                   
                   
                   
                   
                   
                 10679 
                 10694 
                   
               
               
                   
               
               
                 827106 
                 N/A 
                 N/A 
                 CTGCCACCCTACGCAC 
                 47 
                 10658 
                 10673 
                 1015 
               
               
                   
                   
                   
                   
                   
                 10681 
                 10696 
                   
               
               
                   
               
               
                 827107 
                 N/A 
                 N/A 
                 CCTGCCACCCTACGCA 
                 40 
                 10659 
                 10674 
                 1016 
               
               
                   
                   
                   
                   
                   
                 10682 
                 10697 
                   
               
               
                   
               
               
                 827120 
                 N/A 
                 N/A 
                 CCACATGGTGCCCCAG 
                 61 
                 11248 
                 11263 
                 1017 
               
               
                   
               
               
                 827121 
                 N/A 
                 N/A 
                 TTTTAGGAGGGCCACA 
                 126 
                 11259 
                 11274 
                 1018 
               
               
                   
               
               
                 827122 
                 N/A 
                 N/A 
                 GCCCTCTGGTCCGTCC 
                 87 
                 11291 
                 11306 
                 1019 
               
               
                   
               
               
                 827123 
                 N/A 
                 N/A 
                 GGTCAGACAGCACTCC 
                 33 
                 11319 
                 11334 
                 1020 
               
               
                   
               
               
                 827124 
                 N/A 
                 N/A 
                 AGCTAGCAAATGGGTC 
                 82 
                 11331 
                 11346 
                 1021 
               
               
                   
               
               
                 827125 
                 N/A 
                 N/A 
                 TTCCAGTTGGCACAGC 
                 42 
                 11344 
                 11359 
                 1022 
               
               
                   
               
               
                 827126 
                 N/A 
                 N/A 
                 CACAATTGTCATTCCC 
                 22 
                 11395 
                 11410 
                 1023 
               
               
                   
               
               
                 827127 
                 N/A 
                 N/A 
                 TGTTAGCTAACACAAT 
                 48 
                 11405 
                 11420 
                 1024 
               
               
                   
               
               
                 827140 
                 N/A 
                 N/A 
                 CCCACAGAAAACGGAA 
                 40 
                 11690 
                 11705 
                 1025 
               
               
                   
               
               
                 827141 
                 N/A 
                 N/A 
                 GGCTGCTGCATGATTC 
                 24 
                 11738 
                 11753 
                 1026 
               
               
                   
               
               
                 827142 
                 N/A 
                 N/A 
                 ACCAGAATAGATTCAC 
                 108 
                 11766 
                 11781 
                 1027 
               
               
                   
               
               
                 827143 
                 N/A 
                 N/A 
                 TCGAATCGAGTGCCCC 
                 35 
                 11791 
                 11806 
                 1028 
               
               
                   
               
               
                 827144 
                 N/A 
                 N/A 
                 AACAATGAACCTCGAA 
                 98 
                 11802 
                 11817 
                 1029 
               
               
                   
               
               
                 827145 
                 N/A 
                 N/A 
                 TGGTATTAGAATGTAC 
                 29 
                 11881 
                 11896 
                 1030 
               
               
                   
               
               
                 827146 
                 N/A 
                 N/A 
                 GTGGTATTAGAATGTA 
                 41 
                 11882 
                 11897 
                 1031 
               
               
                   
               
               
                 827147 
                 N/A 
                 N/A 
                 TGTGGTATTAGAATGT 
                 23 
                 11883 
                 11898 
                 1032 
               
               
                   
               
               
                 827160 
                 N/A 
                 N/A 
                 GTGCAGGGTCTTACTT 
                 55 
                 12230 
                 12245 
                 1033 
               
               
                   
               
               
                 827161 
                 N/A 
                 N/A 
                 AAATACCAGTGCAGGG 
                 73 
                 12238 
                 12253 
                 1034 
               
               
                   
               
               
                 827162 
                 N/A 
                 N/A 
                 GTACATCAATTATGCC 
                 47 
                 12268 
                 12283 
                 1035 
               
               
                   
               
               
                 827163 
                 N/A 
                 N/A 
                 GGGCACTCAAGATTTG 
                 52 
                 12295 
                 12310 
                 1036 
               
               
                   
               
               
                 827164 
                 N/A 
                 N/A 
                 CAAACCTGAGTGGGCA 
                 43 
                 12306 
                 12321 
                 1037 
               
               
                   
               
               
                 827165 
                 N/A 
                 N/A 
                 CTCGACTGTCAAACCT 
                 50 
                 12315 
                 12330 
                 1038 
               
               
                   
               
               
                 827166 
                 N/A 
                 N/A 
                 GTTCAACATCTCGACT 
                 44 
                 12324 
                 12339 
                 1039 
               
               
                   
               
               
                 827167 
                 N/A 
                 N/A 
                 GTAATGGGAGTGTTCA 
                 18 
                 12335 
                 12350 
                 1040 
               
               
                   
               
               
                 827180 
                 N/A 
                 N/A 
                 GTTGAAGGTGTGTGTT 
                 35 
                 13095 
                 13110 
                 1041 
               
               
                   
               
               
                 827181 
                 N/A 
                 N/A 
                 AGCAACTCAAAGGTGT 
                 38 
                 13111 
                 13126 
                 1042 
               
               
                   
               
               
                 827182 
                 N/A 
                 N/A 
                 AGATTTGTACATGAGG 
                 78 
                 13481 
                 13496 
                 1043 
               
               
                   
               
               
                 827183 
                 N/A 
                 N/A 
                 ACCCGAAACACATTAG 
                 66 
                 13504 
                 13519 
                 1044 
               
               
                   
               
               
                 827184 
                 N/A 
                 N/A 
                 GTTTAGGCCGCACCCG 
                 27 
                 13515 
                 13530 
                 1045 
               
               
                   
               
               
                 827185 
                 N/A 
                 N/A 
                 ATTTACGGTGTTTAGG 
                 61 
                 13524 
                 13539 
                 1046 
               
               
                   
               
               
                 827186 
                 N/A 
                 N/A 
                 GGGATTTACAGTGAGC 
                 40 
                 13548 
                 13563 
                 1047 
               
               
                   
               
               
                 827187 
                 N/A 
                 N/A 
                 AAAGCATATGCCCCCA 
                 26 
                 13678 
                 13693 
                 1048 
               
               
                   
               
               
                 827200 
                 N/A 
                 N/A 
                 AACCGTATGTAGTAGG 
                 27 
                 14153 
                 14168 
                 1049 
               
               
                   
               
               
                 827201 
                 N/A 
                 N/A 
                 CAACCGTATGTAGTAG 
                 53 
                 14154 
                 14169 
                 1050 
               
               
                   
               
               
                 827202 
                 N/A 
                 N/A 
                 ACAACCGTATGTAGTA 
                 39 
                 14155 
                 14170 
                 1051 
               
               
                   
               
               
                 827203 
                 N/A 
                 N/A 
                 AACAACCGTATGTAGT 
                 50 
                 14156 
                 14171 
                 1052 
               
               
                   
               
               
                 827204 
                 N/A 
                 N/A 
                 GAACAACCGTATGTAG 
                 53 
                 14157 
                 14172 
                 1053 
               
               
                   
               
               
                 827205 
                 N/A 
                 N/A 
                 AGAACAACCGTATGTA 
                 43 
                 14158 
                 14173 
                 1054 
               
               
                   
               
               
                 827206 
                 N/A 
                 N/A 
                 TAGAACAACCGTATGT 
                 46 
                 14159 
                 14174 
                 1055 
               
               
                   
               
               
                 827219 
                 N/A 
                 N/A 
                 TGACATACTGCTTCTA 
                 54 
                 14642 
                 14657 
                 1056 
               
               
                   
               
               
                 827220 
                 N/A 
                 N/A 
                 CCCCAGCAGGTATTTT 
                 156 
                 14667 
                 14682 
                 1057 
               
               
                   
               
               
                 827221 
                 N/A 
                 N/A 
                 CCCAAGCAATCACCAG 
                 120 
                 14737 
                 14752 
                 1058 
               
               
                   
               
               
                 827222 
                 N/A 
                 N/A 
                 GACCAAAAGTGTGCCA 
                 45 
                 14831 
                 14846 
                 1059 
               
               
                   
               
               
                 827223 
                 N/A 
                 N/A 
                 GACACAATCGCCGCTC 
                 114 
                 14905 
                 14920 
                 1060 
               
               
                   
               
               
                 827224 
                 N/A 
                 N/A 
                 GAATAAGTGGAGATAT 
                 55 
                 15017 
                 15032 
                 1061 
               
               
                   
               
               
                 827225 
                 N/A 
                 N/A 
                 TGCATTTCCGTCTCAA 
                 21 
                 15204 
                 15219 
                 1062 
               
               
                   
               
               
                 827226 
                 N/A 
                 N/A 
                 GGGTATCGAGACCACC 
                 116 
                 15441 
                 15456 
                 1063 
               
               
                   
               
               
                 827239 
                 N/A 
                 N/A 
                 CCGGACCTAGAAGGGA 
                 117 
                 15987 
                 16002 
                 1064 
               
               
                   
               
               
                 827240 
                 N/A 
                 N/A 
                 GGCCACGGCGAGCCCA 
                 153 
                 16080 
                 16095 
                 1065 
               
               
                   
               
               
                 827241 
                 N/A 
                 N/A 
                 GTAAACAGGTGTGTCC 
                 63 
                 16110 
                 16125 
                 1066 
               
               
                   
               
               
                 827242 
                 N/A 
                 N/A 
                 CTGGAGCGAGTGTCTG 
                 165 
                 16238 
                 16253 
                 1067 
               
               
                   
               
               
                 827243 
                 N/A 
                 N/A 
                 GCGGAGCCCATGGGTG 
                 73 
                 16616 
                 16631 
                 1068 
               
               
                   
               
               
                 827244 
                 N/A 
                 N/A 
                 TGTCACTGGGCTGCGC 
                 60 
                 16650 
                 16665 
                 1069 
               
               
                   
               
               
                 827245 
                 N/A 
                 N/A 
                 CCGCGAGCCCACGAGG 
                 54 
                 16676 
                 16691 
                 1070 
               
               
                   
               
               
                 827246 
                 N/A 
                 N/A 
                 CACCAAGAGGTGTTAT 
                 54 
                 16738 
                 16753 
                 1071 
               
               
                   
               
               
                 827259 
                 N/A 
                 N/A 
                 ATTTATACCTCCCCTG 
                 101 
                 17495 
                 17510 
                 1072 
               
               
                   
               
               
                 827260 
                 N/A 
                 N/A 
                 CACACACGGTTTTGGT 
                 18 
                 17513 
                 17528 
                 1073 
               
               
                   
               
               
                 827261 
                 N/A 
                 N/A 
                 GACCAGTAGCTGCACA 
                 72 
                 17527 
                 17542 
                 1074 
               
               
                   
               
               
                 827262 
                 N/A 
                 N/A 
                 ATTAAGGGAGTTGCAG 
                 106 
                 17555 
                 17570 
                 1075 
               
               
                   
               
               
                 827263 
                 N/A 
                 N/A 
                 CCCTAGGAGCATGGAC 
                 56 
                 17585 
                 17600 
                 1076 
               
               
                   
               
               
                 827264 
                 N/A 
                 N/A 
                 GCAGAAGTCCCTAGGA 
                 92 
                 17593 
                 17608 
                 1077 
               
               
                   
               
               
                 827265 
                 N/A 
                 N/A 
                 ACAGGAGTCGGAAGCC 
                 48 
                 17640 
                 17655 
                 1078 
               
               
                   
               
               
                 827266 
                 N/A 
                 N/A 
                 GGGATAAGCCCCTTGG 
                 87 
                 17705 
                 17720 
                 1079 
               
               
                   
               
               
                 827279 
                 N/A 
                 N/A 
                 AGATCCATGCTTCCAG 
                 17 
                 18216 
                 18231 
                 1080 
               
               
                   
               
               
                 827280 
                 N/A 
                 N/A 
                 AAGATCCATGCTTCCA 
                 11 
                 18217 
                 18232 
                 1081 
               
               
                   
               
               
                 827281 
                 N/A 
                 N/A 
                 CCAAGATCCATGCTTC 
                 22 
                 18219 
                 18234 
                 1082 
               
               
                   
               
               
                 827282 
                 N/A 
                 N/A 
                 ACCAAGATCCATGCTT 
                 55 
                 18220 
                 18235 
                 1083 
               
               
                   
               
               
                 827283 
                 N/A 
                 N/A 
                 GACCAAGATCCATGCT 
                 17 
                 18221 
                 18236 
                 1084 
               
               
                   
               
               
                 827284 
                 N/A 
                 N/A 
                 AGACCAAGATCCATGC 
                 12 
                 18222 
                 18237 
                 1085 
               
               
                   
               
               
                 827285 
                 N/A 
                 N/A 
                 AAGACCAAGATCCATG 
                 19 
                 18223 
                 18238 
                 1086 
               
               
                   
               
               
                 827298 
                 N/A 
                 N/A 
                 AGGATGATGTGATACA 
                 30 
                 18735 
                 18750 
                 1087 
               
               
                   
               
               
                 827299 
                 N/A 
                 N/A 
                 AAGGATGATGTGATAC 
                 74 
                 18736 
                 18751 
                 1088 
               
               
                   
               
               
                 827300 
                 N/A 
                 N/A 
                 ATCTAAGAAATAGGCT 
                 35 
                 18755 
                 18770 
                 1089 
               
               
                   
               
               
                 827301 
                 N/A 
                 N/A 
                 CACATAGCCCAGATAG 
                 31 
                 18834 
                 18849 
                 1090 
               
               
                   
               
               
                 827302 
                 N/A 
                 N/A 
                 TGCCAAAGGAGCATGG 
                 61 
                 18901 
                 18916 
                 1091 
               
               
                   
               
               
                 827303 
                 N/A 
                 N/A 
                 CTTGAGTAAAAGTGCC 
                 30 
                 18913 
                 18928 
                 1092 
               
               
                   
               
               
                 827304 
                 N/A 
                 N/A 
                 GTACAGCTCTTGAGAT 
                 46 
                 18955 
                 18970 
                 1093 
               
               
                   
               
               
                 827317 
                 N/A 
                 N/A 
                 GGTAAGAAGTGACACC 
                 57 
                 19364 
                 19379 
                 1094 
               
               
                   
               
               
                 827318 
                 N/A 
                 N/A 
                 GTGTACTGGGCAGAGT 
                 20 
                 19390 
                 19405 
                 1095 
               
               
                   
               
               
                 827319 
                 N/A 
                 N/A 
                 TGCTACCATCTTACTT 
                 52 
                 19463 
                 19478 
                 1096 
               
               
                   
               
               
                 827320 
                 N/A 
                 N/A 
                 GGCTTAGGTGTTGCTA 
                 45 
                 19474 
                 19489 
                 1097 
               
               
                   
               
               
                 827321 
                 N/A 
                 N/A 
                 GCGGACTCAGGCTTAG 
                 51 
                 19483 
                 19498 
                 1098 
               
               
                   
               
               
                 827322 
                 N/A 
                 N/A 
                 TGACAGGTGTGGGCGG 
                 48 
                 19495 
                 19510 
                 1099 
               
               
                   
               
               
                 827323 
                 N/A 
                 N/A 
                 GTGACAGGTGTGGGCG 
                 66 
                 19496 
                 19511 
                 1100 
               
               
                   
               
               
                 827324 
                 N/A 
                 N/A 
                 GTCCAGGTGACAGTTA 
                 38 
                 19522 
                 19537 
                 1101 
               
               
                   
               
               
                 827337 
                 N/A 
                 N/A 
                 CCCAGGCGAGCAATGA 
                 20 
                 19746 
                 19761 
                 1102 
               
               
                   
               
               
                 827338 
                 N/A 
                 N/A 
                 GGTATAACAACCCAGG 
                 25 
                 19756 
                 19771 
                 1103 
               
               
                   
               
               
                 827339 
                 N/A 
                 N/A 
                 CAGTAGGGTGGAGTGG 
                 74 
                 19774 
                 19789 
                 1104 
               
               
                   
               
               
                 827340 
                 N/A 
                 N/A 
                 GTACAAAGGTTCCTGT 
                 74 
                 19829 
                 19844 
                 1105 
               
               
                   
               
               
                 827341 
                 N/A 
                 N/A 
                 CGTGAAGTAAGGTTGA 
                 31 
                 19846 
                 19861 
                 1106 
               
               
                   
               
               
                 827342 
                 N/A 
                 N/A 
                 GTTACATGTGGTGACG 
                 53 
                 19860 
                 19875 
                 1107 
               
               
                   
               
               
                 827343 
                 N/A 
                 N/A 
                 AGTTACATGTGGTGAC 
                 45 
                 19861 
                 19876 
                 1108 
               
               
                   
               
               
                 827344 
                 N/A 
                 N/A 
                 TAGTTACATGTGGTGA 
                 31 
                 19862 
                 19877 
                 1109 
               
               
                   
               
               
                 827356 
                 N/A 
                 N/A 
                 AGGACTAACTGGAATA 
                 27 
                 19876 
                 19891 
                 1110 
               
               
                   
               
               
                 827357 
                 N/A 
                 N/A 
                 CAGGACTAACTGGAAT 
                 31 
                 19877 
                 19892 
                 1111 
               
               
                   
               
               
                 827358 
                 N/A 
                 N/A 
                 GCCCGGTGAGATATTC 
                 80 
                 19923 
                 19938 
                 1112 
               
               
                   
               
               
                 827359 
                 N/A 
                 N/A 
                 CCCGATAGCTGGTTGT 
                 18 
                 20415 
                 20430 
                 1113 
               
               
                   
               
               
                 827360 
                 N/A 
                 N/A 
                 TTAATTAGTTCACCCG 
                 12 
                 20427 
                 20442 
                 1114 
               
               
                   
               
               
                 827361 
                 N/A 
                 N/A 
                 AGTGAATCCTCACACT 
                 161 
                 20444 
                 20459 
                 1115 
               
               
                   
               
               
                 827362 
                 N/A 
                 N/A 
                 CCTAGCTGGGAGGTGT 
                 56 
                 20516 
                 20531 
                 1116 
               
               
                   
               
               
                 827363 
                 N/A 
                 N/A 
                 CATGTTGGAGGTGATC 
                 58 
                 20531 
                 20546 
                 1117 
               
               
                   
               
               
                 827376 
                 N/A 
                 N/A 
                 TCATAGGTAAACACCC 
                 31 
                 21565 
                 21580 
                 1118 
               
               
                   
               
               
                 827377 
                 N/A 
                 N/A 
                 GAAAAGTCTGGTAGCT 
                 23 
                 21628 
                 21643 
                 1119 
               
               
                   
               
               
                 827378 
                 N/A 
                 N/A 
                 TGGTGTGACCATTTGG 
                 13 
                 21643 
                 21658 
                 1120 
               
               
                   
               
               
                 827379 
                 N/A 
                 N/A 
                 ATGGTGTGACCATTTG 
                 7 
                 21644 
                 21659 
                 1121 
               
               
                   
               
               
                 827380 
                 N/A 
                 N/A 
                 AAATGGTGTGACCATT 
                 72 
                 21646 
                 21661 
                 1122 
               
               
                   
               
               
                 827381 
                 N/A 
                 N/A 
                 TAAATGGTGTGACCAT 
                 41 
                 21647 
                 21662 
                 1123 
               
               
                   
               
               
                 827382 
                 N/A 
                 N/A 
                 GTTAAATGGTGTGACC 
                 14 
                 21649 
                 21664 
                 1124 
               
               
                   
               
               
                 827394 
                 N/A 
                 N/A 
                 GTACGATTACAGGGAT 
                 31 
                 21753 
                 21768 
                 1125 
               
               
                   
               
               
                 827395 
                 N/A 
                 N/A 
                 AGTACGATTACAGGGA 
                 5 
                 21754 
                 21769 
                 1126 
               
               
                   
               
               
                 827396 
                 N/A 
                 N/A 
                 TAGTACGATTACAGGG 
                 90 
                 21755 
                 21770 
                 1127 
               
               
                   
               
               
                 827397 
                 N/A 
                 N/A 
                 CTAGTACGATTACAGG 
                 24 
                 21756 
                 21771 
                 1128 
               
               
                   
               
               
                 827398 
                 N/A 
                 N/A 
                 ACTAGTACGATTACAG 
                 29 
                 21757 
                 21772 
                 1129 
               
               
                   
               
               
                 827399 
                 N/A 
                 N/A 
                 CACTAGTACGATTACA 
                 50 
                 21758 
                 21773 
                 1130 
               
               
                   
               
               
                 827400 
                 N/A 
                 N/A 
                 TCACTAGTACGATTAC 
                 78 
                 21759 
                 21774 
                 1131 
               
               
                   
               
               
                 827401 
                 N/A 
                 N/A 
                 CTCACTAGTACGATTA 
                 34 
                 21760 
                 21775 
                 1132 
               
               
                   
               
               
                 827413 
                 N/A 
                 N/A 
                 CCTATGAGAATCAGTA 
                 16 
                 22313 
                 22328 
                 1133 
               
               
                   
               
               
                 827414 
                 N/A 
                 N/A 
                 GCCTATGAGAATCAGT 
                 19 
                 22314 
                 22329 
                 1134 
               
               
                   
               
               
                 827415 
                 N/A 
                 N/A 
                 CTATAGTGGCCTATGA 
                 111 
                 22322 
                 22337 
                 1135 
               
               
                   
               
               
                 827416 
                 N/A 
                 N/A 
                 GATACACACTAAGCAC 
                 23 
                 22342 
                 22357 
                 1136 
               
               
                   
               
               
                 827417 
                 N/A 
                 N/A 
                 AGATACACACTAAGCA 
                 11 
                 22343 
                 22358 
                 1137 
               
               
                   
               
               
                 827418 
                 N/A 
                 N/A 
                 GCACACTACAGCGAGA 
                 55 
                 22455 
                 22470 
                 1138 
               
               
                   
               
               
                 827419 
                 N/A 
                 N/A 
                 AAACATAGAGCTTCGA 
                 7 
                 22721 
                 22736 
                 1139 
               
               
                   
               
               
                 827432 
                 N/A 
                 N/A 
                 GGTGAGCCCTTCGCAC 
                 11 
                 22828 
                 22843 
                 1140 
               
               
                   
               
               
                 827433 
                 N/A 
                 N/A 
                 TGAAGGAGAGGCTACA 
                 39 
                 22866 
                 22881 
                 1141 
               
               
                   
               
               
                 827434 
                 N/A 
                 N/A 
                 ATTCTAGGATGTACTG 
                 53 
                 22926 
                 22941 
                 1142 
               
               
                   
               
               
                 827435 
                 N/A 
                 N/A 
                 GTGACATACTGGTGCA 
                 17 
                 22943 
                 22958 
                 1143 
               
               
                   
               
               
                 827436 
                 N/A 
                 N/A 
                 GGGATATTCCACTGGC 
                 37 
                 22983 
                 22998 
                 1144 
               
               
                   
               
               
                 827437 
                 N/A 
                 N/A 
                 AACTAGGTGATCCGGG 
                 9 
                 22996 
                 23011 
                 1145 
               
               
                   
               
               
                 827438 
                 N/A 
                 N/A 
                 AATGTAGGATGATTCT 
                 29 
                 23030 
                 23045 
                 1146 
               
               
                   
               
               
                 827439 
                 N/A 
                 N/A 
                 TTCTAAGCTTATTCTC 
                 34 
                 23057 
                 23072 
                 1147 
               
               
                   
               
               
                 827451 
                 N/A 
                 N/A 
                 GGTGAGCACGGAGCTG 
                 35 
                 23471 
                 23486 
                 1148 
               
               
                   
               
               
                 827452 
                 N/A 
                 N/A 
                 GGAGAAAGTGTGACCA 
                 57 
                 23489 
                 23504 
                 1149 
               
               
                   
               
               
                 827453 
                 N/A 
                 N/A 
                 GAGCAGGGTTAAAGGA 
                 110 
                 23502 
                 23517 
                 1150 
               
               
                   
               
               
                 827454 
                 N/A 
                 N/A 
                 TGTCATCTAGGAGATA 
                 123 
                 23597 
                 23612 
                 1151 
               
               
                   
               
               
                 827455 
                 N/A 
                 N/A 
                 TTGCATAGATCCTGTC 
                 47 
                 23609 
                 23624 
                 1152 
               
               
                   
               
               
                 827456 
                 N/A 
                 N/A 
                 CTTGATGACAGGAGCC 
                 67 
                 23660 
                 23675 
                 1153 
               
               
                   
               
               
                 827457 
                 N/A 
                 N/A 
                 TTGAATCATGAGCTCC 
                 35 
                 23675 
                 23690 
                 1154 
               
               
                   
               
               
                 827458 
                 N/A 
                 N/A 
                 GCCCATGCATCTAAGT 
                 38 
                 23703 
                 23718 
                 1155 
               
               
                   
               
               
                 827471 
                 N/A 
                 N/A 
                 GGACTATGTGGCACCT 
                 43 
                 24342 
                 24357 
                 1156 
               
               
                   
               
               
                 827472 
                 N/A 
                 N/A 
                 TGGCAACCCCTGAGCT 
                 80 
                 24412 
                 24427 
                 1157 
               
               
                   
               
               
                 827473 
                 N/A 
                 N/A 
                 GTTCAGGAAGACCCGC 
                 166 
                 24437 
                 24452 
                 1158 
               
               
                   
               
               
                 827474 
                 N/A 
                 N/A 
                 GCAGAGGCGGGAATCC 
                 78 
                 24524 
                 24539 
                 1159 
               
               
                   
               
               
                 827475 
                 N/A 
                 N/A 
                 CATCAGGGACAGACCT 
                 78 
                 24564 
                 24579 
                 1160 
               
               
                   
               
               
                 827476 
                 N/A 
                 N/A 
                 CTGCAATCTGAGGCGC 
                 85 
                 24761 
                 24776 
                 1161 
               
               
                   
               
               
                 827477 
                 N/A 
                 N/A 
                 CCCTAAGCATGCCTTG 
                 46 
                 24939 
                 24954 
                 1162 
               
               
                   
               
               
                 827478 
                 N/A 
                 N/A 
                 TTTCAAGGCCACTAGG 
                 109 
                 24974 
                 24989 
                 1163 
               
               
                   
               
               
                 827491 
                 N/A 
                 N/A 
                 ACCTTAGGAGCCATTG 
                 27 
                 26493 
                 26508 
                 1164 
               
               
                   
               
               
                 827492 
                 N/A 
                 N/A 
                 ACCCATGTATCTTCTA 
                 46 
                 26627 
                 26642 
                 1165 
               
               
                   
               
               
                 827493 
                 N/A 
                 N/A 
                 AATGAGACAGACCCAT 
                 62 
                 26637 
                 26652 
                 1166 
               
               
                   
               
               
                 827494 
                 N/A 
                 N/A 
                 GGATACAGTATGTCCA 
                 78 
                 26685 
                 26700 
                 1167 
               
               
                   
               
               
                 827495 
                 N/A 
                 N/A 
                 CTCTACTATTGAATGG 
                 46 
                 26699 
                 26714 
                 1168 
               
               
                   
               
               
                 827496 
                 N/A 
                 N/A 
                 ATTATATACCTCTACT 
                 58 
                 26708 
                 26723 
                 1169 
               
               
                   
               
               
                 827497 
                 N/A 
                 N/A 
                 ATCTTAAACAGGTCCA 
                 16 
                 26745 
                 26760 
                 1170 
               
               
                   
               
               
                 827498 
                 N/A 
                 N/A 
                 ACTGATTGTGCCCTTG 
                 17 
                 26777 
                 26792 
                 1171 
               
               
                   
               
               
                 827511 
                 N/A 
                 N/A 
                 CCAGGAGGCCACGACT 
                 30 
                 27241 
                 27256 
                 1172 
               
               
                   
               
               
                 827512 
                 N/A 
                 N/A 
                 TACAATCCTCTAAGGT 
                 43 
                 27271 
                 27286 
                 1173 
               
               
                   
               
               
                 827513 
                 N/A 
                 N/A 
                 CTGTATACCCTGGGAC 
                 59 
                 27378 
                 27393 
                 1174 
               
               
                   
               
               
                 827514 
                 N/A 
                 N/A 
                 TCTCAGCAATCAATAT 
                 62 
                 27490 
                 27505 
                 1175 
               
               
                   
               
               
                 827515 
                 N/A 
                 N/A 
                 GGGAAGTAAGCCCTAG 
                 47 
                 27559 
                 27574 
                 1176 
               
               
                   
               
               
                 827516 
                 N/A 
                 N/A 
                 GGCTGGAGATCTTTAG 
                 34 
                 27607 
                 27622 
                 1177 
               
               
                   
               
               
                 827517 
                 N/A 
                 N/A 
                 CCCAAATCCCTACCAG 
                 61 
                 27623 
                 27638 
                 1178 
               
               
                   
               
               
                 827518 
                 N/A 
                 N/A 
                 GTCATTATTGCTACTT 
                 17 
                 27675 
                 27690 
                 1179 
               
               
                   
               
               
                 827531 
                 N/A 
                 N/A 
                 CAGAATAGCCGGGCGC 
                 46 
                 28650 
                 28665 
                 1180 
               
               
                   
               
               
                 827532 
                 N/A 
                 N/A 
                 GGCAGACACGAGGGTC 
                 40 
                 28699 
                 28714 
                 1181 
               
               
                   
               
               
                 827533 
                 N/A 
                 N/A 
                 CCATACGGATGAACCT 
                 35 
                 28741 
                 28756 
                 1182 
               
               
                   
               
               
                 827534 
                 N/A 
                 N/A 
                 TACCATACGGATGAAC 
                 20 
                 28743 
                 28758 
                 1183 
               
               
                   
               
               
                 827535 
                 N/A 
                 N/A 
                 CTACCATACGGATGAA 
                 49 
                 28744 
                 28759 
                 1184 
               
               
                   
               
               
                 827536 
                 N/A 
                 N/A 
                 GCTACCATACGGATGA 
                 23 
                 28745 
                 28760 
                 1185 
               
               
                   
               
               
                 827537 
                 N/A 
                 N/A 
                 TGCTACCATACGGATG 
                 59 
                 28746 
                 28761 
                 1186 
               
               
                   
               
               
                 827550 
                 N/A 
                 N/A 
                 AGGGAATTAAGCCACA 
                 13 
                 29501 
                 29516 
                 1187 
               
               
                   
               
               
                 827551 
                 N/A 
                 N/A 
                 GGATACACCAGTGTAA 
                 32 
                 29904 
                 29919 
                 1188 
               
               
                   
               
               
                 827552 
                 N/A 
                 N/A 
                 AGCTAAGTCAGGCGAA 
                 67 
                 29930 
                 29945 
                 1189 
               
               
                   
               
               
                 827553 
                 N/A 
                 N/A 
                 TATGAGTGTGCCTTTG 
                 25 
                 30329 
                 30344 
                 1190 
               
               
                   
               
               
                 827554 
                 N/A 
                 N/A 
                 TTCAAGGTTGCAAGTG 
                 41 
                 30348 
                 30363 
                 1191 
               
               
                   
               
               
                 827555 
                 N/A 
                 N/A 
                 AGCTAAGCCAGGGACA 
                 67 
                 30416 
                 30431 
                 1192 
               
               
                   
               
               
                 827556 
                 N/A 
                 N/A 
                 GCCTTATGAGTGGCAG 
                 54 
                 30456 
                 30471 
                 1193 
               
               
                   
               
               
                 827557 
                 N/A 
                 N/A 
                 CCACTTTACAAGAGCA 
                 22 
                 30492 
                 30507 
                 1194 
               
               
                   
               
               
                 827570 
                 N/A 
                 N/A 
                 ATCAAGGTCACTCCCA 
                 48 
                 30959 
                 30974 
                 1195 
               
               
                   
               
               
                 827571 
                 N/A 
                 N/A 
                 GAAGACCCATTCCTAG 
                 78 
                 30992 
                 31007 
                 1196 
               
               
                   
               
               
                 827572 
                 N/A 
                 N/A 
                 CCATATCGATCCCTCT 
                 132 
                 31115 
                 31130 
                 1197 
               
               
                   
               
               
                 827573 
                 N/A 
                 N/A 
                 GAATTTCCTGGACCTT 
                 84 
                 31142 
                 31157 
                 1198 
               
               
                   
               
               
                 827574 
                 N/A 
                 N/A 
                 GAAATGGTAGAGGATG 
                 46 
                 31157 
                 31172 
                 1199 
               
               
                   
               
               
                 827575 
                 N/A 
                 N/A 
                 AGGCACGACCTACCGT 
                 139 
                 31272 
                 31287 
                 1200 
               
               
                   
               
               
                 827576 
                 N/A 
                 N/A 
                 CTCCATCCAGGCACGA 
                 55 
                 31280 
                 31295 
                 1201 
               
               
                   
               
               
                 827577 
                 N/A 
                 N/A 
                 AAGTAAGACCCCCAGA 
                 79 
                 31306 
                 31321 
                 1202 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 SEQ 
                 SEQ 
                   
                   
                 SEQ 
                 SEQ 
                   
               
               
                   
                 ID: 1 
                 ID: 1 
                   
                 α-ENaC 
                 ID: 2 
                 ID 2: 
                 SEQ 
               
               
                 Compound 
                 Start 
                 Stop 
                   
                 (% 
                 Start 
                 Stop 
                 ID 
               
               
                 Number 
                 Site 
                 Site 
                 Sequence 
                 control) 
                 Site 
                 Site 
                 NO 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 668182 
                 535 
                 550 
                 CCAGAAGGCCGTCTTC 
                 34 
                 5463 
                 5478 
                 1203 
               
               
                   
               
               
                 668208 
                 764 
                 779 
                 ATTTGTACAGGTCAAA 
                 35 
                 16349 
                 16364 
                 1204 
               
               
                   
               
               
                 668218 
                 974 
                 989 
                 ATTTGTTCTGGTTGCA 
                 19 
                 17760 
                 17775 
                 1205 
               
               
                   
               
               
                 826072 
                 7 
                 22 
                 GCTTTAGACGCAGACA 
                 102 
                 4268 
                 4283 
                 1206 
               
               
                   
               
               
                 826073 
                 9 
                 24 
                 GGGCTTTAGACGCAGA 
                 68 
                 4270 
                 4285 
                 1207 
               
               
                   
               
               
                 826082 
                 37 
                 52 
                 TGGACCTGAGAAGGCG 
                 58 
                 4298 
                 4313 
                 1208 
               
               
                   
               
               
                 826083 
                 40 
                 55 
                 TACTGGACCTGAGAAG 
                 66 
                 4301 
                 4316 
                 1209 
               
               
                   
               
               
                 826084 
                 42 
                 57 
                 AGTACTGGACCTGAGA 
                 55 
                 4303 
                 4318 
                 1210 
               
               
                   
               
               
                 826085 
                 44 
                 59 
                 GGAGTACTGGACCTGA 
                 55 
                 4305 
                 4320 
                 1211 
               
               
                   
               
               
                 826086 
                 45 
                 60 
                 GGGAGTACTGGACCTG 
                 62 
                 4306 
                 4321 
                 1212 
               
               
                   
               
               
                 826087 
                 46 
                 61 
                 TGGGAGTACTGGACCT 
                 81 
                 4307 
                 4322 
                 1213 
               
               
                   
               
               
                 826088 
                 47 
                 62 
                 CTGGGAGTACTGGACC 
                 59 
                 4308 
                 4323 
                 1214 
               
               
                   
               
               
                 826089 
                 50 
                 65 
                 GAACTGGGAGTACTGG 
                 81 
                 4311 
                 4326 
                 1215 
               
               
                   
               
               
                 826092 
                 62 
                 77 
                 CCGAGGGCAGGTGAAC 
                 99 
                 4323 
                 4338 
                 1216 
               
               
                   
               
               
                 826093 
                 72 
                 87 
                 AGGAGGGCTCCCGAGG 
                 81 
                 4333 
                 4348 
                 1217 
               
               
                   
               
               
                 826102 
                 94 
                 109 
                 GAGCCGGGAGTTTTCC 
                 45 
                 4355 
                 4370 
                 1218 
               
               
                   
               
               
                 826103 
                 95 
                 110 
                 AGAGCCGGGAGTTTTC 
                 71 
                 4356 
                 4371 
                 1219 
               
               
                   
               
               
                 826104 
                 98 
                 113 
                 GTCAGAGCCGGGAGTT 
                 73 
                 4359 
                 4374 
                 1220 
               
               
                   
               
               
                 826105 
                 99 
                 114 
                 AGTCAGAGCCGGGAGT 
                 73 
                 4360 
                 4375 
                 1221 
               
               
                   
               
               
                 826106 
                 100 
                 115 
                 GAGTCAGAGCCGGGAG 
                 51 
                 4361 
                 4376 
                 1222 
               
               
                   
               
               
                 826107 
                 101 
                 116 
                 GGAGTCAGAGCCGGGA 
                 53 
                 4362 
                 4377 
                 1223 
               
               
                   
               
               
                 826108 
                 138 
                 153 
                 AATTAAAGGTGAGCAG 
                 67 
                 4399 
                 4414 
                 1224 
               
               
                   
               
               
                 826109 
                 143 
                 158 
                 ATCTCAATTAAAGGTG 
                 91 
                 4404 
                 4419 
                 1225 
               
               
                   
               
               
                 826112 
                 164 
                 179 
                 AGCGACAGGAATCTCA 
                 79 
                 4425 
                 4440 
                 1226 
               
               
                   
               
               
                 826113 
                 166 
                 181 
                 GAAGCGACAGGAATCT 
                 68 
                 4427 
                 4442 
                 1227 
               
               
                   
               
               
                 826122 
                 181 
                 196 
                 GGCCGGCCAGGGATGG 
                 41 
                 4442 
                 4457 
                 1228 
               
               
                   
               
               
                 826123 
                 182 
                 197 
                 TGGCCGGCCAGGGATG 
                 55 
                 4443 
                 4458 
                 1229 
               
               
                   
               
               
                 826124 
                 183 
                 198 
                 CTGGCCGGCCAGGGAT 
                 69 
                 4444 
                 4459 
                 1230 
               
               
                   
               
               
                 826125 
                 187 
                 202 
                 CCCGCTGGCCGGCCAG 
                 32 
                 4448 
                 4463 
                 1231 
               
               
                   
               
               
                 826126 
                 188 
                 203 
                 GCCCGCTGGCCGGCCA 
                 90 
                 4449 
                 4464 
                 1232 
               
               
                   
               
               
                 826127 
                 190 
                 205 
                 CCGCCCGCTGGCCGGC 
                 58 
                 4451 
                 4466 
                 1233 
               
               
                   
               
               
                 826128 
                 192 
                 207 
                 GCCCGCCCGCTGGCCG 
                 81 
                 4453 
                 4468 
                 1234 
               
               
                   
               
               
                 826129 
                 194 
                 209 
                 GAGCCCGCCCGCTGGC 
                 57 
                 4455 
                 4470 
                 1235 
               
               
                   
               
               
                 826132 
                 217 
                 232 
                 ACAGGTGCAGCGGCCT 
                 76 
                 4478 
                 4493 
                 1236 
               
               
                   
               
               
                 826133 
                 224 
                 239 
                 TCCCCTGACAGGTGCA 
                 78 
                 N/A 
                 N/A 
                 1237 
               
               
                   
               
               
                 826142 
                 256 
                 271 
                 CAGAGGTCTAGGGTCC 
                 26 
                 5184 
                 5199 
                 1238 
               
               
                   
               
               
                 826143 
                 259 
                 274 
                 CTGCAGAGGTCTAGGG 
                 36 
                 5187 
                 5202 
                 1239 
               
               
                   
               
               
                 826144 
                 267 
                 282 
                 GGTATGGGCTGCAGAG 
                 24 
                 5195 
                 5210 
                 1240 
               
               
                   
               
               
                 826145 
                 269 
                 284 
                 CTGGTATGGGCTGCAG 
                 31 
                 5197 
                 5212 
                 1241 
               
               
                   
               
               
                 826146 
                 272 
                 287 
                 GACCTGGTATGGGCTG 
                 30 
                 5200 
                 5215 
                 1242 
               
               
                   
               
               
                 826147 
                 275 
                 290 
                 TGAGACCTGGTATGGG 
                 38 
                 5203 
                 5218 
                 1243 
               
               
                   
               
               
                 826148 
                 278 
                 293 
                 CCATGAGACCTGGTAT 
                 50 
                 5206 
                 5221 
                 1244 
               
               
                   
               
               
                 826149 
                 280 
                 295 
                 CTCCATGAGACCTGGT 
                 42 
                 5208 
                 5223 
                 1245 
               
               
                   
               
               
                 826152 
                 284 
                 299 
                 TCCCCTCCATGAGACC 
                 50 
                 5212 
                 5227 
                 1246 
               
               
                   
               
               
                 826153 
                 286 
                 301 
                 GTTCCCCTCCATGAGA 
                 65 
                 5214 
                 5229 
                 1247 
               
               
                   
               
               
                 826162 
                 332 
                 347 
                 GCCCTGGAGTGGACTG 
                 46 
                 5260 
                 5275 
                 1248 
               
               
                   
               
               
                 826163 
                 333 
                 348 
                 AGCCCTGGAGTGGACT 
                 41 
                 5261 
                 5276 
                 1249 
               
               
                   
               
               
                 826164 
                 343 
                 358 
                 CCCCTTCATGAGCCCT 
                 30 
                 5271 
                 5286 
                 1250 
               
               
                   
               
               
                 826165 
                 344 
                 359 
                 TCCCCTTCATGAGCCC 
                 30 
                 5152 
                 5167 
                 1251 
               
               
                   
                   
                   
                   
                   
                 5272 
                 5287 
                   
               
               
                   
               
               
                 826166 
                 345 
                 360 
                 TTCCCCTTCATGAGCC 
                 29 
                 5153 
                 5168 
                 1252 
               
               
                   
                   
                   
                   
                   
                 5273 
                 5288 
                   
               
               
                   
               
               
                 826167 
                 350 
                 365 
                 GCTTGTTCCCCTTCAT 
                 12 
                 5158 
                 5173 
                 1253 
               
               
                   
                   
                   
                   
                   
                 5278 
                 5293 
                   
               
               
                   
               
               
                 826168 
                 351 
                 366 
                 CGCTTGTTCCCCTTCA 
                 19 
                 5279 
                 5294 
                 1254 
               
               
                   
               
               
                 826169 
                 352 
                 367 
                 ACGCTTGTTCCCCTTC 
                 20 
                 5280 
                 5295 
                 1255 
               
               
                   
               
               
                 826172 
                 356 
                 371 
                 CCTCACGCTTGTTCCC 
                 41 
                 5284 
                 5299 
                 1256 
               
               
                   
               
               
                 826173 
                 359 
                 374 
                 GCTCCTCACGCTTGTT 
                 48 
                 5287 
                 5302 
                 1257 
               
               
                   
               
               
                 826182 
                 425 
                 440 
                 ACTCGATCAGGGCCTC 
                 34 
                 5353 
                 5368 
                 1258 
               
               
                   
               
               
                 826183 
                 427 
                 442 
                 GAACTCGATCAGGGCC 
                 21 
                 5355 
                 5370 
                 1259 
               
               
                   
               
               
                 826184 
                 429 
                 444 
                 TGGAACTCGATCAGGG 
                 45 
                 5357 
                 5372 
                 1260 
               
               
                   
               
               
                 826185 
                 431 
                 446 
                 GGTGGAACTCGATCAG 
                 26 
                 5359 
                 5374 
                 1261 
               
               
                   
               
               
                 826186 
                 432 
                 447 
                 CGGTGGAACTCGATCA 
                 36 
                 5360 
                 5375 
                 1262 
               
               
                   
               
               
                 826187 
                 433 
                 448 
                 GCGGTGGAACTCGATC 
                 47 
                 5361 
                 5376 
                 1263 
               
               
                   
               
               
                 826188 
                 435 
                 450 
                 GAGCGGTGGAACTCGA 
                 39 
                 5363 
                 5378 
                 1264 
               
               
                   
               
               
                 826189 
                 436 
                 451 
                 GGAGCGGTGGAACTCG 
                 53 
                 5364 
                 5379 
                 1265 
               
               
                   
               
               
                 826192 
                 443 
                 458 
                 CTCGGTAGGAGCGGTG 
                 50 
                 5371 
                 5386 
                 1266 
               
               
                   
               
               
                 826193 
                 445 
                 460 
                 CTCTCGGTAGGAGCGG 
                 42 
                 5373 
                 5388 
                 1267 
               
               
                   
               
               
                 826202 
                 516 
                 531 
                 CGGTTGTGCTGGGAGC 
                 19 
                 5444 
                 5459 
                 1268 
               
               
                   
               
               
                 826203 
                 517 
                 532 
                 GCGGTTGTGCTGGGAG 
                 24 
                 5445 
                 5460 
                 1269 
               
               
                   
               
               
                 826204 
                 519 
                 534 
                 ATGCGGTTGTGCTGGG 
                 23 
                 5447 
                 5462 
                 1270 
               
               
                   
               
               
                 826205 
                 524 
                 539 
                 TCTTCATGCGGTTGTG 
                 31 
                 5452 
                 5467 
                 1271 
               
               
                   
               
               
                 826206 
                 529 
                 544 
                 GGCCGTCTTCATGCGG 
                 39 
                 5457 
                 5472 
                 1272 
               
               
                   
               
               
                 826207 
                 532 
                 547 
                 GAAGGCCGTCTTCATG 
                 27 
                 5460 
                 5475 
                 1273 
               
               
                   
               
               
                 826208 
                 564 
                 579 
                 ATGCCAAAGGTGCAGA 
                 51 
                 5492 
                 5507 
                 1274 
               
               
                   
               
               
                 826211 
                 569 
                 584 
                 ACATCATGCCAAAGGT 
                 33 
                 5497 
                 5512 
                 1275 
               
               
                   
               
               
                 826212 
                 573 
                 588 
                 CAGTACATCATGCCAA 
                 42 
                 5501 
                 5516 
                 1276 
               
               
                   
               
               
                 826221 
                 590 
                 605 
                 AAAGCAGGCCGAATTG 
                 38 
                 5518 
                 5533 
                 1277 
               
               
                   
               
               
                 826222 
                 594 
                 609 
                 CCGAAAAGCAGGCCGA 
                 27 
                 5522 
                 5537 
                 1278 
               
               
                   
               
               
                 826223 
                 596 
                 611 
                 CTCCGAAAAGCAGGCC 
                 31 
                 5524 
                 5539 
                 1279 
               
               
                   
               
               
                 826224 
                 598 
                 613 
                 CTCTCCGAAAAGCAGG 
                 37 
                 5526 
                 5541 
                 1280 
               
               
                   
               
               
                 826225 
                 599 
                 614 
                 ACTCTCCGAAAAGCAG 
                 27 
                 5527 
                 5542 
                 1281 
               
               
                   
               
               
                 826226 
                 601 
                 616 
                 GTACTCTCCGAAAAGC 
                 20 
                 5529 
                 5544 
                 1282 
               
               
                   
               
               
                 826227 
                 602 
                 617 
                 AGTACTCTCCGAAAAG 
                 43 
                 5530 
                 5545 
                 1283 
               
               
                   
               
               
                 826228 
                 605 
                 620 
                 TGAAGTACTCTCCGAA 
                 44 
                 5533 
                 5548 
                 1284 
               
               
                   
               
               
                 826231 
                 610 
                 625 
                 GTAGCTGAAGTACTCT 
                 35 
                 5538 
                 5553 
                 1285 
               
               
                   
               
               
                 826232 
                 611 
                 626 
                 GGTAGCTGAAGTACTC 
                 23 
                 5539 
                 5554 
                 1286 
               
               
                   
               
               
                 826241 
                 650 
                 665 
                 CGAGCTTGTCCGAGTT 
                 17 
                 5578 
                 5593 
                 1287 
               
               
                   
               
               
                 826242 
                 652 
                 667 
                 GACGAGCTTGTCCGAG 
                 22 
                 5580 
                 5595 
                 1288 
               
               
                   
               
               
                 826243 
                 653 
                 668 
                 AGACGAGCTTGTCCGA 
                 23 
                 5581 
                 5596 
                 1289 
               
               
                   
               
               
                 826244 
                 655 
                 670 
                 GAAGACGAGCTTGTCC 
                 30 
                 5583 
                 5598 
                 1290 
               
               
                   
               
               
                 826245 
                 656 
                 671 
                 GGAAGACGAGCTTGTC 
                 27 
                 5584 
                 5599 
                 1291 
               
               
                   
               
               
                 826246 
                 657 
                 672 
                 GGGAAGACGAGCTTGT 
                 21 
                 5585 
                 5600 
                 1292 
               
               
                   
               
               
                 826247 
                 699 
                 714 
                 TCCGGGTACCTGTAGG 
                 32 
                 N/A 
                 N/A 
                 1293 
               
               
                   
               
               
                 826248 
                 700 
                 715 
                 TTCCGGGTACCTGTAG 
                 38 
                 N/A 
                 N/A 
                 1294 
               
               
                   
               
               
                 826251 
                 705 
                 720 
                 TTAATTTCCGGGTACC 
                 27 
                 16290 
                 16305 
                 1295 
               
               
                   
               
               
                 826252 
                 709 
                 724 
                 CTCTTTAATTTCCGGG 
                 31 
                 16294 
                 16309 
                 1296 
               
               
                   
               
               
                 826261 
                 763 
                 778 
                 TTTGTACAGGTCAAAG 
                 43 
                 16348 
                 16363 
                 1297 
               
               
                   
               
               
                 826262 
                 766 
                 781 
                 GTATTTGTACAGGTCA 
                 12 
                 16351 
                 16366 
                 1298 
               
               
                   
               
               
                 826263 
                 777 
                 792 
                 GTGAAGGAGCTGTATT 
                 93 
                 16362 
                 16377 
                 1299 
               
               
                   
               
               
                 826264 
                 786 
                 801 
                 ACGAGAGTGGTGAAGG 
                 43 
                 16371 
                 16386 
                 1300 
               
               
                   
               
               
                 826265 
                 788 
                 803 
                 CCACGAGAGTGGTGAA 
                 56 
                 16373 
                 16388 
                 1301 
               
               
                   
               
               
                 826266 
                 789 
                 804 
                 GCCACGAGAGTGGTGA 
                 77 
                 16374 
                 16389 
                 1302 
               
               
                   
               
               
                 826269 
                 794 
                 809 
                 AGCCGGCCACGAGAGT 
                 43 
                 16379 
                 16394 
                 1303 
               
               
                   
               
               
                 826270 
                 795 
                 810 
                 GAGCCGGCCACGAGAG 
                 42 
                 16380 
                 16395 
                 1304 
               
               
                   
               
               
                 826279 
                 812 
                 827 
                 GGTCGCGACGGCTGCG 
                 31 
                 16397 
                 16412 
                 1305 
               
               
                   
               
               
                 826280 
                 815 
                 830 
                 GCAGGTCGCGACGGCT 
                 48 
                 16400 
                 16415 
                 1306 
               
               
                   
               
               
                 826281 
                 816 
                 831 
                 CGCAGGTCGCGACGGC 
                 35 
                 16401 
                 16416 
                 1307 
               
               
                   
               
               
                 826282 
                 819 
                 834 
                 CCCCGCAGGTCGCGAC 
                 49 
                 16404 
                 16419 
                 1308 
               
               
                   
               
               
                 826283 
                 820 
                 835 
                 CCCCCGCAGGTCGCGA 
                 43 
                 16405 
                 16420 
                 1309 
               
               
                   
               
               
                 826284 
                 821 
                 836 
                 TCCCCCGCAGGTCGCG 
                 35 
                 16406 
                 16421 
                 1310 
               
               
                   
               
               
                 826285 
                 822 
                 837 
                 GTCCCCCGCAGGTCGC 
                 49 
                 16407 
                 16422 
                 1311 
               
               
                   
               
               
                 826286 
                 824 
                 839 
                 GAGTCCCCCGCAGGTC 
                 67 
                 16409 
                 16424 
                 1312 
               
               
                   
               
               
                 826289 
                 831 
                 846 
                 TGCGGCAGAGTCCCCC 
                 37 
                 16416 
                 16431 
                 1313 
               
               
                   
               
               
                 826290 
                 833 
                 848 
                 GGTGCGGCAGAGTCCC 
                 38 
                 16418 
                 16433 
                 1314 
               
               
                   
               
               
                 826299 
                 893 
                 908 
                 CCACGCTACGGGCTCG 
                 33 
                 16478 
                 16493 
                 1315 
               
               
                   
               
               
                 826300 
                 895 
                 910 
                 GGCCACGCTACGGGCT 
                 56 
                 16480 
                 16495 
                 1316 
               
               
                   
               
               
                 826301 
                 897 
                 912 
                 GAGGCCACGCTACGGG 
                 36 
                 16482 
                 16497 
                 1317 
               
               
                   
               
               
                 826302 
                 898 
                 913 
                 GGAGGCCACGCTACGG 
                 34 
                 16483 
                 16498 
                 1318 
               
               
                   
               
               
                 826303 
                 900 
                 915 
                 CTGGAGGCCACGCTAC 
                 49 
                 16485 
                 16500 
                 1319 
               
               
                   
               
               
                 826304 
                 902 
                 917 
                 AGCTGGAGGCCACGCT 
                 28 
                 16487 
                 16502 
                 1320 
               
               
                   
               
               
                 826305 
                 908 
                 923 
                 CCCGCAAGCTGGAGGC 
                 41 
                 16493 
                 16508 
                 1321 
               
               
                   
               
               
                 826306 
                 913 
                 928 
                 GTTGTCCCGCAAGCTG 
                 23 
                 16498 
                 16513 
                 1322 
               
               
                   
               
               
                 826309 
                 917 
                 932 
                 GGTTGTTGTCCCGCAA 
                 33 
                 16502 
                 16517 
                 1323 
               
               
                   
               
               
                 826310 
                 918 
                 933 
                 GGGTTGTTGTCCCGCA 
                 43 
                 16503 
                 16518 
                 1324 
               
               
                   
               
               
                 826319 
                 971 
                 986 
                 TGTTCTGGTTGCACAG 
                 35 
                 N/A 
                 N/A 
                 1325 
               
               
                   
               
               
                 826320 
                 972 
                 987 
                 TTGTTCTGGTTGCACA 
                 38 
                 N/A 
                 N/A 
                 1326 
               
               
                   
               
               
                 826321 
                 973 
                 988 
                 TTTGTTCTGGTTGCAC 
                 23 
                 17759 
                 17774 
                 1327 
               
               
                   
               
               
                 826322 
                 975 
                 990 
                 GATTTGTTCTGGTTGC 
                 36 
                 17761 
                 17776 
                 1328 
               
               
                   
               
               
                 826323 
                 976 
                 991 
                 CGATTTGTTCTGGTTG 
                 22 
                 17762 
                 17777 
                 1329 
               
               
                   
               
               
                 826324 
                 978 
                 993 
                 TCCGATTTGTTCTGGT 
                 38 
                 17764 
                 17779 
                 1330 
               
               
                   
               
               
                 826327 
                 981 
                 996 
                 CAGTCCGATTTGTTCT 
                 36 
                 17767 
                 17782 
                 1331 
               
               
                   
               
               
                 826328 
                 982 
                 997 
                 GCAGTCCGATTTGTTC 
                 34 
                 17768 
                 17783 
                 1332 
               
               
                   
               
               
                 826337 
                 1000 
                 1015 
                 TGAGTATGTCTGGTAG 
                 19 
                 17786 
                 17801 
                 1333 
               
               
                   
               
               
                 826338 
                 1001 
                 1016 
                 ATGAGTATGTCTGGTA 
                 15 
                 17787 
                 17802 
                 1334 
               
               
                   
               
               
                 826339 
                 1003 
                 1018 
                 TGATGAGTATGTCTGG 
                 19 
                 17789 
                 17804 
                 1335 
               
               
                   
               
               
                 826340 
                 1007 
                 1022 
                 CCCCTGATGAGTATGT 
                 41 
                 17793 
                 17808 
                 1336 
               
               
                   
               
               
                 826341 
                 1009 
                 1024 
                 CACCCCTGATGAGTAT 
                 61 
                 17795 
                 17810 
                 1337 
               
               
                   
               
               
                 826342 
                 1011 
                 1026 
                 TCCACCCCTGATGAGT 
                 36 
                 17797 
                 17812 
                 1338 
               
               
                   
               
               
                 826343 
                 1014 
                 1029 
                 GCATCCACCCCTGATG 
                 56 
                 17800 
                 17815 
                 1339 
               
               
                   
               
               
                 826344 
                 1015 
                 1030 
                 CGCATCCACCCCTGAT 
                 70 
                 17801 
                 17816 
                 1340 
               
               
                   
               
               
                 826347 
                 1019 
                 1034 
                 TCACCGCATCCACCCC 
                 35 
                 17805 
                 17820 
                 1341 
               
               
                   
               
               
                 826348 
                 1021 
                 1036 
                 CCTCACCGCATCCACC 
                 72 
                 17807 
                 17822 
                 1342 
               
               
                   
               
               
                 826357 
                 1047 
                 1062 
                 TTGATGTAGTGGAAGC 
                 39 
                 17833 
                 17848 
                 1343 
               
               
                   
               
               
                 826358 
                 1058 
                 1073 
                 TCGACAGGATGTTGAT 
                 56 
                 17844 
                 17859 
                 1344 
               
               
                   
               
               
                 826359 
                 1060 
                 1075 
                 CCTCGACAGGATGTTG 
                 28 
                 17846 
                 17861 
                 1345 
               
               
                   
               
               
                 826360 
                 1061 
                 1076 
                 GCCTCGACAGGATGTT 
                 37 
                 17847 
                 17862 
                 1346 
               
               
                   
               
               
                 826361 
                 1064 
                 1079 
                 GCAGCCTCGACAGGAT 
                 45 
                 17850 
                 17865 
                 1347 
               
               
                   
               
               
                 826362 
                 1065 
                 1080 
                 GGCAGCCTCGACAGGA 
                 22 
                 17851 
                 17866 
                 1348 
               
               
                   
               
               
                 826363 
                 1074 
                 1089 
                 AGAGTCTCTGGCAGCC 
                 25 
                 17860 
                 17875 
                 1349 
               
               
                   
               
               
                 826364 
                 1110 
                 1125 
                 ATGAAGTTGCCCAGCG 
                 64 
                 17896 
                 17911 
                 1350 
               
               
                   
               
               
                 826367 
                 1117 
                 1132 
                 GGCGAAGATGAAGTTG 
                 47 
                 17903 
                 17918 
                 1351 
               
               
                   
               
               
                 826368 
                 1121 
                 1136 
                 GGCAGGCGAAGATGAA 
                 46 
                 17907 
                 17922 
                 1352 
               
               
                   
               
               
                 826377 
                 1148 
                 1163 
                 CCTGGTTGCAGGAGAC 
                 24 
                 17934 
                 17949 
                 1353 
               
               
                   
               
               
                 826378 
                 1150 
                 1165 
                 CGCCTGGTTGCAGGAG 
                 38 
                 17936 
                 17951 
                 1354 
               
               
                   
               
               
                 826379 
                 1152 
                 1167 
                 TTCGCCTGGTTGCAGG 
                 54 
                 N/A 
                 N/A 
                 1355 
               
               
                   
               
               
                 826380 
                 1153 
                 1168 
                 ATTCGCCTGGTTGCAG 
                 52 
                 N/A 
                 N/A 
                 1356 
               
               
                   
               
               
                 826381 
                 1155 
                 1170 
                 TAATTCGCCTGGTTGC 
                 86 
                 N/A 
                 N/A 
                 1357 
               
               
                   
               
               
                 826382 
                 1157 
                 1172 
                 AGTAATTCGCCTGGTT 
                 73 
                 N/A 
                 N/A 
                 1358 
               
               
                   
               
               
                 826383 
                 1158 
                 1173 
                 GAGTAATTCGCCTGGT 
                 49 
                 N/A 
                 N/A 
                 1359 
               
               
                   
               
               
                 826384 
                 1160 
                 1175 
                 GAGAGTAATTCGCCTG 
                 63 
                 N/A 
                 N/A 
                 1360 
               
               
                   
               
               
                 826387 
                 1168 
                 1183 
                 GTGGAAGTGAGAGTAA 
                 48 
                 24124 
                 24139 
                 1361 
               
               
                   
               
               
                 826388 
                 1230 
                 1245 
                 GACATCCAGAGGTTGG 
                 47 
                 24186 
                 24201 
                 1362 
               
               
                   
               
               
                 826397 
                 1261 
                 1276 
                 GGACAGACCGTTGTTG 
                 31 
                 N/A 
                 N/A 
                 1363 
               
               
                   
               
               
                 826398 
                 1267 
                 1282 
                 CATCAGGGACAGACCG 
                 40 
                 N/A 
                 N/A 
                 1364 
               
               
                   
               
               
                 826399 
                 1268 
                 1283 
                 GCATCAGGGACAGACC 
                 24 
                 24565 
                 24580 
                 1365 
               
               
                   
               
               
                 826400 
                 1273 
                 1288 
                 GCGCAGCATCAGGGAC 
                 28 
                 24570 
                 24585 
                 1366 
               
               
                   
               
               
                 826401 
                 1275 
                 1290 
                 GCGCGCAGCATCAGGG 
                 37 
                 24572 
                 24587 
                 1367 
               
               
                   
               
               
                 826402 
                 1277 
                 1292 
                 CTGCGCGCAGCATCAG 
                 35 
                 24574 
                 24589 
                 1368 
               
               
                   
               
               
                 826403 
                 1279 
                 1294 
                 CTCTGCGCGCAGCATC 
                 20 
                 24576 
                 24591 
                 1369 
               
               
                   
               
               
                 826404 
                 1280 
                 1295 
                 GCTCTGCGCGCAGCAT 
                 49 
                 24577 
                 24592 
                 1370 
               
               
                   
               
               
                 826407 
                 1283 
                 1298 
                 TCTGCTCTGCGCGCAG 
                 65 
                 24580 
                 24595 
                 1371 
               
               
                   
               
               
                 826408 
                 1284 
                 1299 
                 TTCTGCTCTGCGCGCA 
                 49 
                 24581 
                 24596 
                 1372 
               
               
                   
               
               
                 826417 
                 1333 
                 1348 
                 CACCATTACCCGGGCC 
                 34 
                 24630 
                 24645 
                 1373 
               
               
                   
               
               
                 826418 
                 1341 
                 1356 
                 TGCCCGTGCACCATTA 
                 30 
                 24638 
                 24653 
                 1374 
               
               
                   
               
               
                 826419 
                 1343 
                 1358 
                 CCTGCCCGTGCACCAT 
                 25 
                 24640 
                 24655 
                 1375 
               
               
                   
               
               
                 826420 
                 1344 
                 1359 
                 TCCTGCCCGTGCACCA 
                 18 
                 24641 
                 24656 
                 1376 
               
               
                   
               
               
                 826421 
                 1345 
                 1360 
                 ATCCTGCCCGTGCACC 
                 31 
                 24642 
                 24657 
                 1377 
               
               
                   
               
               
                 826422 
                 1348 
                 1363 
                 TTCATCCTGCCCGTGC 
                 20 
                 24645 
                 24660 
                 1378 
               
               
                   
               
               
                 826423 
                 1350 
                 1365 
                 GGTTCATCCTGCCCGT 
                 53 
                 24647 
                 24662 
                 1379 
               
               
                   
               
               
                 826424 
                 1351 
                 1366 
                 AGGTTCATCCTGCCCG 
                 78 
                 24648 
                 24663 
                 1380 
               
               
                   
               
               
                 826427 
                 1358 
                 1373 
                 TAAAGGCAGGTTCATC 
                 89 
                 24655 
                 24670 
                 1381 
               
               
                   
               
               
                 826428 
                 1361 
                 1376 
                 CCATAAAGGCAGGTTC 
                 73 
                 24658 
                 24673 
                 1382 
               
               
                   
               
               
                 826437 
                 1393 
                 1408 
                 CACGCCAGGCCGCAAG 
                 36 
                 24690 
                 24705 
                 1383 
               
               
                   
               
               
                 826438 
                 1394 
                 1409 
                 CCACGCCAGGCGGCAA 
                 45 
                 24691 
                 24706 
                 1384 
               
               
                   
               
               
                 826439 
                 1395 
                 1410 
                 TCCACGCCAGGCCGCA 
                 28 
                 24692 
                 24707 
                 1385 
               
               
                   
               
               
                 826440 
                 1396 
                 1411 
                 CTCCACGCCAGGCCGC 
                 38 
                 24693 
                 24708 
                 1386 
               
               
                   
               
               
                 826441 
                 1399 
                 1414 
                 GGTCTCCACGCCAGGC 
                 38 
                 24696 
                 24711 
                 1387 
               
               
                   
               
               
                 826442 
                 1401 
                 1416 
                 GAGGTCTCCACGCCAG 
                 25 
                 24698 
                 24713 
                 1388 
               
               
                   
               
               
                 826443 
                 1402 
                 1417 
                 GGAGGTCTCCACGCCA 
                 55 
                 24699 
                 24714 
                 1389 
               
               
                   
               
               
                 826444 
                 1404 
                 1419 
                 ATGGAGGTCTCCACGC 
                 45 
                 24701 
                 24716 
                 1390 
               
               
                   
               
               
                 826447 
                 1442 
                 1457 
                 CGCCCCCAAGTCTGTC 
                 38 
                 25162 
                 25177 
                 1391 
               
               
                   
               
               
                 826448 
                 1443 
                 1458 
                 TCGCCCCCAAGTCTGT 
                 32 
                 25163 
                 25178 
                 1392 
               
               
                   
               
               
                 826457 
                 1461 
                 1476 
                 TTGGTGCAGTCGCCAT 
                 25 
                 25181 
                 25196 
                 1393 
               
               
                   
               
               
                 826458 
                 1462 
                 1477 
                 CTTGGTGCAGTCGCCA 
                 43 
                 25182 
                 25197 
                 1394 
               
               
                   
               
               
                 826459 
                 1463 
                 1478 
                 TCTTGGTGCAGTCGCC 
                 24 
                 25183 
                 25198 
                 1395 
               
               
                   
               
               
                 826460 
                 1466 
                 1481 
                 CATTCTTGGTGCAGTC 
                 40 
                 25186 
                 25201 
                 1396 
               
               
                   
               
               
                 826461 
                 1468 
                 1483 
                 GCCATTCTTGGTGCAG 
                 45 
                 25188 
                 25203 
                 1397 
               
               
                   
               
               
                 826462 
                 1470 
                 1485 
                 CTGCCATTCTTGGTGC 
                 30 
                 25190 
                 25205 
                 1398 
               
               
                   
               
               
                 826463 
                 1480 
                 1495 
                 AGGAACATCACTGCCA 
                 29 
                 25200 
                 25215 
                 1399 
               
               
                   
               
               
                 826464 
                 1496 
                 1511 
                 GGTAAAGGTTCTCAAC 
                 68 
                 25216 
                 25231 
                 1400 
               
               
                   
               
               
                 826467 
                 1509 
                 1524 
                 GTGTACTTTGAAGGGT 
                 49 
                 25229 
                 25244 
                 1401 
               
               
                   
               
               
                 826468 
                 1526 
                 1541 
                 GAATACACACCTGCTG 
                 54 
                 N/A 
                 N/A 
                 1402 
               
               
                   
               
               
                 826477 
                 1558 
                 1573 
                 CTCCTTGATCATGCTC 
                 31 
                 25472 
                 25487 
                 1403 
               
               
                   
               
               
                 826478 
                 1559 
                 1574 
                 ACTCCTTGATCATGCT 
                 36 
                 25473 
                 25488 
                 1404 
               
               
                   
               
               
                 826479 
                 1560 
                 1575 
                 CACTCCTTGATCATGC 
                 29 
                 25474 
                 25489 
                 1405 
               
               
                   
               
               
                 826480 
                 1561 
                 1576 
                 ACACTCCTTGATCATG 
                 29 
                 25475 
                 25490 
                 1406 
               
               
                   
               
               
                 826481 
                 1562 
                 1577 
                 CACACTCCTTGATCAT 
                 38 
                 25476 
                 25491 
                 1407 
               
               
                   
               
               
                 826482 
                 1564 
                 1579 
                 GCCACACTCCTTGATC 
                 32 
                 25478 
                 25493 
                 1408 
               
               
                   
               
               
                 826483 
                 1576 
                 1591 
                 GATGTAGGCACAGCCA 
                 25 
                 25490 
                 25505 
                 1409 
               
               
                   
               
               
                 826484 
                 1577 
                 1592 
                 AGATGTAGGCACAGCC 
                 22 
                 25491 
                 25506 
                 1410 
               
               
                   
               
               
                 826486 
                 1581 
                 1596 
                 TAGAAGATGTAGGCAC 
                 47 
                 25495 
                 25510 
                 1411 
               
               
                   
               
               
                 826487 
                 1582 
                 1597 
                 ATAGAAGATGTAGGCA 
                 49 
                 25496 
                 25511 
                 1412 
               
               
                   
               
               
                 826496 
                 1638 
                 1653 
                 TACCCCCAGGAACTGT 
                 27 
                 N/A 
                 N/A 
                 1413 
               
               
                   
               
               
                 826497 
                 1639 
                 1654 
                 GTACCCCCAGGAACTG 
                 49 
                 N/A 
                 N/A 
                 1414 
               
               
                   
               
               
                 826498 
                 1640 
                 1655 
                 AGTACCCCCAGGAACT 
                 65 
                 N/A 
                 N/A 
                 1415 
               
               
                   
               
               
                 826499 
                 1641 
                 1656 
                 CAGTACCCCCAGGAAC 
                 44 
                 N/A 
                 N/A 
                 1416 
               
               
                   
               
               
                 826500 
                 1648 
                 1663 
                 ATAGTAGCAGTACCCC 
                 36 
                 N/A 
                 N/A 
                 1417 
               
               
                   
               
               
                 826501 
                 1650 
                 1665 
                 TTATAGTAGCAGTACC 
                 38 
                 30596 
                 30611 
                 1418 
               
               
                   
               
               
                 826502 
                 1654 
                 1669 
                 GAGCTTATAGTAGCAG 
                 59 
                 30600 
                 30615 
                 1419 
               
               
                   
               
               
                 826503 
                 1655 
                 1670 
                 GGAGCTTATAGTAGCA 
                 55 
                 30601 
                 30616 
                 1420 
               
               
                   
               
               
                 826506 
                 1662 
                 1677 
                 TCAACCTGGAGCTTAT 
                 39 
                 30608 
                 30623 
                 1421 
               
               
                   
               
               
                 826507 
                 1664 
                 1679 
                 AGTCAACCTGGAGCTT 
                 41 
                 30610 
                 30625 
                 1422 
               
               
                   
               
               
                 826516 
                 1717 
                 1732 
                 CACGCTGCATGGCTTC 
                 21 
                 N/A 
                 N/A 
                 1423 
               
               
                   
               
               
                 826517 
                 1720 
                 1735 
                 GGTCACGCTGCATGGC 
                 38 
                 N/A 
                 N/A 
                 1424 
               
               
                   
               
               
                 826518 
                 1722 
                 1737 
                 CTGGTCACGCTGCATG 
                 35 
                 N/A 
                 N/A 
                 1425 
               
               
                   
               
               
                 826519 
                 1723 
                 1738 
                 GCTGGTCACGCTGCAT 
                 30 
                 N/A 
                 N/A 
                 1426 
               
               
                   
               
               
                 826520 
                 1724 
                 1739 
                 AGCTGGTCACGCTGCA 
                 41 
                 N/A 
                 N/A 
                 1427 
               
               
                   
               
               
                 826521 
                 1727 
                 1742 
                 GGTAGCTGGTCACGCT 
                 26 
                 30778 
                 30793 
                 1428 
               
               
                   
               
               
                 826522 
                 1729 
                 1744 
                 CTGGTAGCTGGTCACG 
                 46 
                 30780 
                 30795 
                 1429 
               
               
                   
               
               
                 826523 
                 1732 
                 1747 
                 GAGCTGGTAGCTGGTC 
                 48 
                 30783 
                 30798 
                 1430 
               
               
                   
               
               
                 826526 
                 1737 
                 1752 
                 GCAGAGAGCTGGTAGC 
                 67 
                 30788 
                 30803 
                 1431 
               
               
                   
               
               
                 826527 
                 1749 
                 1764 
                 CGTGAGTAACCAGCAG 
                 48 
                 30800 
                 30815 
                 1432 
               
               
                   
               
               
                 826556 
                 1890 
                 1905 
                 GACTCAGAATTGGTTT 
                 61 
                 31246 
                 31261 
                 1433 
               
               
                   
               
               
                 826557 
                 1961 
                 1976 
                 CCGAGGAGCCGAACCA 
                 50 
                 31790 
                 31805 
                 1434 
               
               
                   
               
               
                 826558 
                 1965 
                 1980 
                 AACACCGAGGAGCCGA 
                 14 
                 31794 
                 31809 
                 1435 
               
               
                   
               
               
                 826559 
                 1966 
                 1981 
                 CAACACCGAGGAGCCG 
                 36 
                 31795 
                 31810 
                 1436 
               
               
                   
               
               
                 826560 
                 1968 
                 1983 
                 GACAACACCGAGGAGC 
                 56 
                 31797 
                 31812 
                 1437 
               
               
                   
               
               
                 826561 
                 1970 
                 1985 
                 CAGACAACACCGAGGA 
                 30 
                 31799 
                 31814 
                 1438 
               
               
                   
               
               
                 826562 
                 1972 
                 1987 
                 CACAGACAACACCGAG 
                 42 
                 31801 
                 31816 
                 1439 
               
               
                   
               
               
                 826563 
                 1973 
                 1988 
                 CCACAGACAACACCGA 
                 60 
                 31802 
                 31817 
                 1440 
               
               
                   
               
               
                 826566 
                 1998 
                 2013 
                 TCAAAGACGAGCTCAG 
                 61 
                 31827 
                 31842 
                 1441 
               
               
                   
               
               
                 826567 
                 1999 
                 2014 
                 GTCAAAGACGAGCTCA 
                 62 
                 31828 
                 31843 
                 1442 
               
               
                   
               
               
                 826576 
                 2036 
                 2051 
                 ACCTTCGGAGCAGCAT 
                 17 
                 31865 
                 31880 
                 1443 
               
               
                   
               
               
                 826577 
                 2038 
                 2053 
                 GAACCTTCGGAGCAGC 
                 28 
                 31867 
                 31882 
                 1444 
               
               
                   
               
               
                 826578 
                 2039 
                 2054 
                 GGAACCTTCGGAGCAG 
                 17 
                 31868 
                 31883 
                 1445 
               
               
                   
               
               
                 826579 
                 2040 
                 2055 
                 CGGAACCTTCGGAGCA 
                 20 
                 31869 
                 31884 
                 1446 
               
               
                   
               
               
                 826580 
                 2041 
                 2056 
                 TCGGAACCTTCGGAGC 
                 32 
                 31870 
                 31885 
                 1447 
               
               
                   
               
               
                 826581 
                 2042 
                 2057 
                 TTCGGAACCTTCGGAG 
                 30 
                 31871 
                 31886 
                 1448 
               
               
                   
               
               
                 826582 
                 2043 
                 2058 
                 CTTCGGAACCTTCGGA 
                 47 
                 31872 
                 31887 
                 1449 
               
               
                   
               
               
                 826583 
                 2044 
                 2059 
                 GCTTCGGAACCTTCGG 
                 30 
                 31873 
                 31888 
                 1450 
               
               
                   
               
               
                 826585 
                 2047 
                 2062 
                 TCGGCTTCGGAACCTT 
                 32 
                 31876 
                 31891 
                 1451 
               
               
                   
               
               
                 826586 
                 2048 
                 2063 
                 ATCGGCTTCGGAACCT 
                 33 
                 31877 
                 31892 
                 1452 
               
               
                   
               
               
                 826595 
                 2059 
                 2074 
                 TGGAGACCAGTATCGG 
                 20 
                 31888 
                 31903 
                 1453 
               
               
                   
               
               
                 826596 
                 2061 
                 2076 
                 CCTGGAGACCAGTATC 
                 30 
                 31890 
                 31905 
                 1454 
               
               
                   
               
               
                 826597 
                 2066 
                 2081 
                 CTCGGCCTGGAGACCA 
                 35 
                 31895 
                 31910 
                 1455 
               
               
                   
               
               
                 826598 
                 2069 
                 2084 
                 CCCCTCGGCCTGGAGA 
                 42 
                 31898 
                 31913 
                 1456 
               
               
                   
               
               
                 826599 
                 2071 
                 2086 
                 GCCCCCTCGGCCTGGA 
                 64 
                 31900 
                 31915 
                 1457 
               
               
                   
               
               
                 826600 
                 2072 
                 2087 
                 TGCCCCCTCGGCCTGG 
                 46 
                 31901 
                 31916 
                 1458 
               
               
                   
               
               
                 826601 
                 2093 
                 2108 
                 AGGCTACCTCCTGAGC 
                 48 
                 31922 
                 31937 
                 1459 
               
               
                   
               
               
                 826602 
                 2098 
                 2113 
                 GGTGGAGGCTACCTCC 
                 62 
                 31927 
                 31942 
                 1460 
               
               
                   
               
               
                 826605 
                 2279 
                 2294 
                 GCCCCCCCAGAGGACA 
                 90 
                 32108 
                 32123 
                 1461 
               
               
                   
               
               
                 826606 
                 2280 
                 2295 
                 GGCCCCCCCAGAGGAC 
                 75 
                 32109 
                 32124 
                 1462 
               
               
                   
               
               
                 826615 
                 2320 
                 2335 
                 GCATCTGCCTTGGTGT 
                 24 
                 32149 
                 32164 
                 1463 
               
               
                   
               
               
                 826616 
                 2322 
                 2337 
                 GAGCATCTGCCTTGGT 
                 24 
                 32151 
                 32166 
                 1464 
               
               
                   
               
               
                 826617 
                 2324 
                 2339 
                 AGGAGCATCTGCCTTG 
                 32 
                 32153 
                 32168 
                 1465 
               
               
                   
               
               
                 826618 
                 2330 
                 2345 
                 CACCAGAGGAGCATCT 
                 46 
                 32159 
                 32174 
                 1466 
               
               
                   
               
               
                 826619 
                 2331 
                 2346 
                 CCACCAGAGGAGCATC 
                 34 
                 32160 
                 32175 
                 1467 
               
               
                   
               
               
                 826620 
                 2355 
                 2370 
                 AATCTTGCCAGGGCCA 
                 21 
                 32184 
                 32199 
                 1468 
               
               
                   
               
               
                 826621 
                 2356 
                 2371 
                 CAATCTTGCCAGGGCC 
                 37 
                 32185 
                 32200 
                 1469 
               
               
                   
               
               
                 826622 
                 2359 
                 2374 
                 CTTCAATCTTGCCAGG 
                 41 
                 32188 
                 32203 
                 1470 
               
               
                   
               
               
                 826625 
                 2393 
                 2408 
                 GTTTGGGCGGCTCTGA 
                 36 
                 32222 
                 32237 
                 1471 
               
               
                   
               
               
                 826626 
                 2395 
                 2410 
                 CAGTTTGGGCGGCTCT 
                 22 
                 32224 
                 32239 
                 1472 
               
               
                   
               
               
                 826635 
                 2412 
                 2427 
                 CCTCCACACATCAACG 
                 38 
                 32241 
                 32256 
                 1473 
               
               
                   
               
               
                 826636 
                 2435 
                 2450 
                 GAGCCCTTACCCATCT 
                 50 
                 32264 
                 32279 
                 1474 
               
               
                   
               
               
                 826637 
                 2436 
                 2451 
                 TGAGCCCTTACCCATC 
                 49 
                 32265 
                 32280 
                 1475 
               
               
                   
               
               
                 826638 
                 2439 
                 2454 
                 TCCTGAGCCCTTACCC 
                 33 
                 32268 
                 32283 
                 1476 
               
               
                   
               
               
                 826639 
                 2447 
                 2462 
                 GAGCAACTTCCTGAGC 
                 28 
                 32276 
                 32291 
                 1477 
               
               
                   
               
               
                 826640 
                 2449 
                 2464 
                 TGGAGCAACTTCCTGA 
                 39 
                 32278 
                 32293 
                 1478 
               
               
                   
               
               
                 826641 
                 2459 
                 2474 
                 CTACTGTTCTTGGAGC 
                 28 
                 32288 
                 32303 
                 1479 
               
               
                   
               
               
                 826642 
                 2462 
                 2477 
                 CAGCTACTGTTCTTGG 
                 22 
                 32291 
                 32306 
                 1480 
               
               
                   
               
               
                 826645 
                 2477 
                 2492 
                 TCTGGGCAGCTTCATC 
                 37 
                 32306 
                 32321 
                 1481 
               
               
                   
               
               
                 826646 
                 2490 
                 2505 
                 GAGCCAAGGCACTTCT 
                 31 
                 32319 
                 32334 
                 1482 
               
               
                   
               
               
                 826655 
                 2553 
                 2568 
                 CTTAGCCGCAGTTGGG 
                 13 
                 32382 
                 32397 
                 1483 
               
               
                   
               
               
                 826656 
                 2554 
                 2569 
                 ACTTAGCCGCAGTTGG 
                 35 
                 32383 
                 32398 
                 1484 
               
               
                   
               
               
                 826657 
                 2555 
                 2570 
                 GACTTAGCCGCAGTTG 
                 40 
                 32384 
                 32399 
                 1485 
               
               
                   
               
               
                 826658 
                 2556 
                 2571 
                 AGACTTAGCCGCAGTT 
                 24 
                 32385 
                 32400 
                 1486 
               
               
                   
               
               
                 826659 
                 2557 
                 2572 
                 GAGACTTAGCCGCAGT 
                 19 
                 32386 
                 32401 
                 1487 
               
               
                   
               
               
                 826660 
                 2559 
                 2574 
                 AAGAGACTTAGCCGCA 
                 18 
                 32388 
                 32403 
                 1488 
               
               
                   
               
               
                 826661 
                 2561 
                 2576 
                 AAAAGAGACTTAGCCG 
                 42 
                 32390 
                 32405 
                 1489 
               
               
                   
               
               
                 826663 
                 2577 
                 2592 
                 TGGCTGATCCAAGGGA 
                 46 
                 32406 
                 32421 
                 1490 
               
               
                   
               
               
                 826664 
                 2578 
                 2593 
                 TTGGCTGATCCAAGGG 
                 48 
                 32407 
                 32422 
                 1491 
               
               
                   
               
               
                 826673 
                 2587 
                 2602 
                 AAGTTTCGCTTGGCTG 
                 18 
                 32416 
                 32431 
                 1492 
               
               
                   
               
               
                 826674 
                 2589 
                 2604 
                 CCAAGTTTCGCTTGGC 
                 63 
                 32418 
                 32433 
                 1493 
               
               
                   
               
               
                 826675 
                 2591 
                 2606 
                 CTCCAAGTTTCGCTTG 
                 33 
                 32420 
                 32435 
                 1494 
               
               
                   
               
               
                 826676 
                 2593 
                 2608 
                 AGCTCCAAGTTTCGCT 
                 48 
                 32422 
                 32437 
                 1495 
               
               
                   
               
               
                 826677 
                 2600 
                 2615 
                 TTGTCAAAGCTCCAAG 
                 19 
                 32429 
                 32444 
                 1496 
               
               
                   
               
               
                 826678 
                 2602 
                 2617 
                 CCTTGTCAAAGCTCCA 
                 8 
                 32431 
                 32446 
                 1497 
               
               
                   
               
               
                 826679 
                 2604 
                 2619 
                 TTCCTTGTCAAAGCTC 
                 24 
                 32433 
                 32448 
                 1498 
               
               
                   
               
               
                 826680 
                 2607 
                 2622 
                 AAGTTCCTTGTCAAAG 
                 39 
                 32436 
                 32451 
                 1499 
               
               
                   
               
               
                 826683 
                 2621 
                 2636 
                 GCGGTTTCTTAGGAAA 
                 22 
                 32450 
                 32465 
                 1500 
               
               
                   
               
               
                 826684 
                 2622 
                 2637 
                 AGCGGTTTCTTAGGAA 
                 26 
                 32451 
                 32466 
                 1501 
               
               
                   
               
               
                 826693 
                 2653 
                 2668 
                 GTACCCTTGGTTGTGT 
                 21 
                 32482 
                 32497 
                 1502 
               
               
                   
               
               
                 826694 
                 2655 
                 2670 
                 GTGTACCCTTGGTTGT 
                 31 
                 32484 
                 32499 
                 1503 
               
               
                   
               
               
                 826695 
                 2656 
                 2671 
                 CGTGTACCCTTGGTTG 
                 22 
                 32485 
                 32500 
                 1504 
               
               
                   
               
               
                 826696 
                 2670 
                 2685 
                 CCCGTGCATGCCTGCG 
                 23 
                 32499 
                 32514 
                 1505 
               
               
                   
               
               
                 826697 
                 2674 
                 2689 
                 GAAACCCGTGCATGCC 
                 28 
                 32503 
                 32518 
                 1506 
               
               
                   
               
               
                 826698 
                 2676 
                 2691 
                 AGGAAACCCGTGCATG 
                 21 
                 32505 
                 32520 
                 1507 
               
               
                   
               
               
                 826699 
                 2677 
                 2692 
                 CAGGAAACCCGTGCAT 
                 31 
                 32506 
                 32521 
                 1508 
               
               
                   
               
               
                 826700 
                 2678 
                 2693 
                 GCAGGAAACCCGTGCA 
                 58 
                 32507 
                 32522 
                 1509 
               
               
                   
               
               
                 826703 
                 2685 
                 2700 
                 TCGCTGGGCAGGAAAC 
                 31 
                 32514 
                 32529 
                 1510 
               
               
                   
               
               
                 826704 
                 2687 
                 2702 
                 CGTCGCTGGGCAGGAA 
                 29 
                 32516 
                 32531 
                 1511 
               
               
                   
               
               
                 826713 
                 2736 
                 2751 
                 GTGCTACTGGAGAGCA 
                 34 
                 32565 
                 32580 
                 1512 
               
               
                   
               
               
                 826714 
                 2737 
                 2752 
                 TGTGCTACTGGAGAGC 
                 35 
                 32566 
                 32581 
                 1513 
               
               
                   
               
               
                 826715 
                 2738 
                 2753 
                 CTGTGCTACTGGAGAG 
                 19 
                 32567 
                 32582 
                 1514 
               
               
                   
               
               
                 826716 
                 2739 
                 2754 
                 TCTGTGCTACTGGAGA 
                 23 
                 32568 
                 32583 
                 1515 
               
               
                   
               
               
                 826717 
                 2740 
                 2755 
                 ATCTGTGCTACTGGAG 
                 19 
                 32569 
                 32584 
                 1516 
               
               
                   
               
               
                 826718 
                 2750 
                 2765 
                 AGGAGCAGACATCTGT 
                 17 
                 32579 
                 32594 
                 1517 
               
               
                   
               
               
                 826719 
                 2775 
                 2790 
                 GGGTTTCCCACCCAAG 
                 91 
                 32604 
                 32619 
                 1518 
               
               
                   
               
               
                 826720 
                 2810 
                 2825 
                 GGAATTGCCTAAGTAA 
                 29 
                 32639 
                 32654 
                 1519 
               
               
                   
               
               
                 826723 
                 2837 
                 2852 
                 GCCCTAGCCCTCGGGA 
                 66 
                 32666 
                 32681 
                 1520 
               
               
                   
               
               
                 826724 
                 2839 
                 2854 
                 TAGCCCTAGCCCTCGG 
                 60 
                 32668 
                 32683 
                 1521 
               
               
                   
               
               
                 826733 
                 2860 
                 2875 
                 TTTACTTACCCGGGTC 
                 40 
                 32689 
                 32704 
                 1522 
               
               
                   
               
               
                 826734 
                 2862 
                 2877 
                 CCTTTACTTACCCGGG 
                 47 
                 32691 
                 32706 
                 1523 
               
               
                   
               
               
                 826735 
                 2865 
                 2880 
                 CTGCCTTTACTTACCC 
                 37 
                 32694 
                 32709 
                 1524 
               
               
                   
               
               
                 826736 
                 2884 
                 2899 
                 GGCTAGAGGAGCCCTG 
                 48 
                 32713 
                 32728 
                 1525 
               
               
                   
               
               
                 826737 
                 2886 
                 2901 
                 GAGGCTAGAGGAGCCC 
                 46 
                 32715 
                 32730 
                 1526 
               
               
                   
               
               
                 826738 
                 2891 
                 2906 
                 GGTATGAGGCTAGAGG 
                 27 
                 32720 
                 32735 
                 1527 
               
               
                   
               
               
                 826739 
                 2893 
                 2908 
                 CGGGTATGAGGCTAGA 
                 34 
                 32722 
                 32737 
                 1528 
               
               
                   
               
               
                 826740 
                 2895 
                 2910 
                 CACGGGTATGAGGCTA 
                 55 
                 32724 
                 32739 
                 1529 
               
               
                   
               
               
                 826743 
                 2951 
                 2966 
                 CATGTAGAGGTATGAA 
                 26 
                 32780 
                 32795 
                 1530 
               
               
                   
               
               
                 826744 
                 2953 
                 2968 
                 GACATGTAGAGGTATG 
                 31 
                 32782 
                 32797 
                 1531 
               
               
                   
               
               
                 826753 
                 2966 
                 2981 
                 AATATCTCAAGCAGAC 
                 18 
                 32795 
                 32810 
                 1532 
               
               
                   
               
               
                 826754 
                 2986 
                 3001 
                 GGAAACTTTCAGGCTG 
                 22 
                 32815 
                 32830 
                 1533 
               
               
                   
               
               
                 826755 
                 2987 
                 3002 
                 GGGAAACTTTCAGGCT 
                 25 
                 32816 
                 32831 
                 1534 
               
               
                   
               
               
                 826756 
                 3001 
                 3016 
                 CTGGCAGATGGTTGGG 
                 31 
                 32830 
                 32845 
                 1535 
               
               
                   
               
               
                 826757 
                 3003 
                 3018 
                 CTCTGGCAGATGGTTG 
                 30 
                 32832 
                 32847 
                 1536 
               
               
                   
               
               
                 826758 
                 3008 
                 3023 
                 GAGTTCTCTGGCAGAT 
                 20 
                 32837 
                 32852 
                 1537 
               
               
                   
               
               
                 826759 
                 3010 
                 3025 
                 AGGAGTTCTCTGGCAG 
                 22 
                 32839 
                 32854 
                 1538 
               
               
                   
               
               
                 826760 
                 3011 
                 3026 
                 TAGGAGTTCTCTGGCA 
                 31 
                 32840 
                 32855 
                 1539 
               
               
                   
               
               
                 826762 
                 3014 
                 3029 
                 GCATAGGAGTTCTCTG 
                 30 
                 32843 
                 32858 
                 1540 
               
               
                   
               
               
                 826763 
                 3015 
                 3030 
                 TGCATAGGAGTTCTCT 
                 18 
                 32844 
                 32859 
                 1541 
               
               
                   
               
               
                 826772 
                 3035 
                 3050 
                 CTGAGCAGGGTTCTAA 
                 20 
                 32864 
                 32879 
                 1542 
               
               
                   
               
               
                 826773 
                 3036 
                 3051 
                 TCTGAGCAGGGTTCTA 
                 24 
                 32865 
                 32880 
                 1543 
               
               
                   
               
               
                 826774 
                 3039 
                 3054 
                 GTGTCTGAGCAGGGTT 
                 13 
                 32868 
                 32883 
                 1544 
               
               
                   
               
               
                 826775 
                 3044 
                 3059 
                 TAATGGTGTCTGAGCA 
                 19 
                 32873 
                 32888 
                 1545 
               
               
                   
               
               
                 826776 
                 3045 
                 3060 
                 GTAATGGTGTCTGAGC 
                 11 
                 32874 
                 32889 
                 1546 
               
               
                   
               
               
                 826777 
                 3074 
                 3089 
                 GACAAGATGTGGCAGA 
                 22 
                 32903 
                 32918 
                 1547 
               
               
                   
               
               
                 826778 
                 3097 
                 3112 
                 GCGGAGTGATCAATTT 
                 50 
                 32926 
                 32941 
                 1548 
               
               
                   
               
               
                 826779 
                 3099 
                 3114 
                 AGGCGGAGTGATCAAT 
                 55 
                 32928 
                 32943 
                 1549 
               
               
                   
               
               
                 826782 
                 3119 
                 3134 
                 TGCTACGGGAGCCCAG 
                 46 
                 32948 
                 32963 
                 1550 
               
               
                   
               
               
                 826783 
                 3120 
                 3135 
                 GTGCTACGGGAGCCCA 
                 24 
                 32949 
                 32964 
                 1551 
               
               
                   
               
               
                 826791 
                 3129 
                 3144 
                 TGTTATAGTGTGCTAC 
                 27 
                 32958 
                 32973 
                 1552 
               
               
                   
               
               
                 826792 
                 3130 
                 3145 
                 ATGTTATAGTGTGCTA 
                 34 
                 32959 
                 32974 
                 1553 
               
               
                   
               
               
                 826793 
                 3131 
                 3146 
                 GATGTTATAGTGTGCT 
                 12 
                 32960 
                 32975 
                 1554 
               
               
                   
               
               
                 826794 
                 3132 
                 3147 
                 AGATGTTATAGTGTGC 
                 13 
                 32961 
                 32976 
                 1555 
               
               
                   
               
               
                 826795 
                 3133 
                 3148 
                 CAGATGTTATAGTGTG 
                 21 
                 32962 
                 32977 
                 1556 
               
               
                   
               
               
                 826796 
                 3135 
                 3150 
                 AGCAGATGTTATAGTG 
                 14 
                 32964 
                 32979 
                 1557 
               
               
                   
               
               
                 826797 
                 3136 
                 3151 
                 CAGCAGATGTTATAGT 
                 37 
                 32965 
                 32980 
                 1558 
               
               
                   
               
               
                 826798 
                 3137 
                 3152 
                 CCAGCAGATGTTATAG 
                 50 
                 32966 
                 32981 
                 1559 
               
               
                   
               
               
                 826801 
                 3146 
                 3161 
                 AGCAACACTCCAGCAG 
                 46 
                 32975 
                 32990 
                 1560 
               
               
                   
               
               
                 826802 
                 3147 
                 3162 
                 CAGCAACACTCCAGCA 
                 35 
                 32976 
                 32991 
                 1561 
               
               
                   
               
               
                 826810 
                 3161 
                 3176 
                 AAAGTATGGTGCAACA 
                 22 
                 32990 
                 33005 
                 1562 
               
               
                   
               
               
                 826811 
                 3162 
                 3177 
                 GAAAGTATGGTGCAAC 
                 19 
                 32991 
                 33006 
                 1563 
               
               
                   
               
               
                 826812 
                 3163 
                 3178 
                 AGAAAGTATGGTGCAA 
                 24 
                 32992 
                 33007 
                 1564 
               
               
                   
               
               
                 826813 
                 3204 
                 3219 
                 GGCACTTACAGTCTAG 
                 16 
                 33033 
                 33048 
                 1565 
               
               
                   
               
               
                 826814 
                 3208 
                 3223 
                 GCAAGGCACTTACAGT 
                 31 
                 33037 
                 33052 
                 1566 
               
               
                   
               
               
                 826815 
                 3210 
                 3225 
                 CCGCAAGGCACTTACA 
                 37 
                 33039 
                 33054 
                 1567 
               
               
                   
               
               
                 826816 
                 3211 
                 3226 
                 ACCGCAAGGCACTTAC 
                 21 
                 33040 
                 33055 
                 1568 
               
               
                   
               
               
                 826819 
                 3214 
                 3229 
                 CTGACCGCAAGGCACT 
                 23 
                 33043 
                 33058 
                 1569 
               
               
                   
               
               
                 826820 
                 3215 
                 3230 
                 CCTGACCGCAAGGCAC 
                 38 
                 33044 
                 33059 
                 1570 
               
               
                   
               
               
                 826829 
                 3226 
                 3241 
                 AAGATTCAGTCCCTGA 
                 27 
                 33055 
                 33070 
                 1571 
               
               
                   
               
               
                 826830 
                 3228 
                 3243 
                 GCAAGATTCAGTCCCT 
                 25 
                 33057 
                 33072 
                 1572 
               
               
                   
               
               
                 826831 
                 3229 
                 3244 
                 GGCAAGATTCAGTCCC 
                 21 
                 33058 
                 33073 
                 1573 
               
               
                   
               
               
                 826832 
                 3230 
                 3245 
                 GGGCAAGATTCAGTCC 
                 41 
                 33059 
                 33074 
                 1574 
               
               
                   
               
               
                 826833 
                 3231 
                 3246 
                 CGGGCAAGATTCAGTC 
                 29 
                 33060 
                 33075 
                 1575 
               
               
                   
               
               
                 826834 
                 3232 
                 3247 
                 ACGGGCAAGATTCAGT 
                 29 
                 33061 
                 33076 
                 1576 
               
               
                   
               
               
                 826835 
                 3233 
                 3248 
                 AACGGGCAAGATTCAG 
                 23 
                 33062 
                 33077 
                 1577 
               
               
                   
               
               
                 826838 
                 3237 
                 3252 
                 CATAAACGGGCAAGAT 
                 47 
                 33066 
                 33081 
                 1578 
               
               
                   
               
               
                 826839 
                 3238 
                 3253 
                 ACATAAACGGGCAAGA 
                 42 
                 33067 
                 33082 
                 1579 
               
               
                   
               
               
                 826848 
                 3256 
                 3271 
                 GGGCTAGACATGGAGC 
                 20 
                 33085 
                 33100 
                 1580 
               
               
                   
               
               
                 826849 
                 3259 
                 3274 
                 GATGGGCTAGACATGG 
                 26 
                 33088 
                 33103 
                 1581 
               
               
                   
               
               
                 826850 
                 3261 
                 3276 
                 ATGATGGGCTAGACAT 
                 34 
                 33090 
                 33105 
                 1582 
               
               
                   
               
               
                 826851 
                 3275 
                 3290 
                 TTGCTCCAAGCAGGAT 
                 39 
                 33104 
                 33119 
                 1583 
               
               
                   
               
               
                 826852 
                 3276 
                 3291 
                 CTTGCTCCAAGCAGGA 
                 75 
                 33105 
                 33120 
                 1584 
               
               
                   
               
               
                 826853 
                 3279 
                 3294 
                 CTACTTGCTCCAAGCA 
                 66 
                 33108 
                 33123 
                 1585 
               
               
                   
               
               
                 826854 
                 3281 
                 3296 
                 GCCTACTTGCTCCAAG 
                 53 
                 33110 
                 33125 
                 1586 
               
               
                   
               
               
                 826855 
                 3292 
                 3307 
                 ATTGAGCTCCTGCCTA 
                 42 
                 33121 
                 33136 
                 1587 
               
               
                   
               
               
                 826858 
                 N/A 
                 N/A 
                 TACCTCCCCTTGGAAG 
                 95 
                 2717 
                 2732 
                 1588 
               
               
                   
               
               
                 826859 
                 N/A 
                 N/A 
                 GATACCTCCCCTTGGA 
                 43 
                 2719 
                 2734 
                 1589 
               
               
                   
               
               
                 826868 
                 N/A 
                 N/A 
                 CCCTTGATACTGCTCA 
                 48 
                 N/A 
                 N/A 
                 1590 
               
               
                   
               
               
                 826869 
                 N/A 
                 N/A 
                 CCCCTTGATACTGCTC 
                 89 
                 N/A 
                 N/A 
                 1591 
               
               
                   
               
               
                 826870 
                 N/A 
                 N/A 
                 TGTTCCCCTTGATACT 
                 61 
                 N/A 
                 N/A 
                 1592 
               
               
                   
               
               
                 826871 
                 N/A 
                 N/A 
                 TTGTTCCCCTTGATAC 
                 70 
                 N/A 
                 N/A 
                 1593 
               
               
                   
               
               
                 826872 
                 N/A 
                 N/A 
                 CTTGTTCCCCTTGATA 
                 27 
                 N/A 
                 N/A 
                 1594 
               
               
                   
               
               
                 826873 
                 N/A 
                 N/A 
                 AGCTTGTTCCCCTTGA 
                 25 
                 N/A 
                 N/A 
                 1595 
               
               
                   
               
               
                 826874 
                 N/A 
                 N/A 
                 CAGCTTGTTCCCCTTG 
                 40 
                 N/A 
                 N/A 
                 1596 
               
               
                   
               
               
                 826875 
                 N/A 
                 N/A 
                 CCAGCTTGTTCCCCTT 
                 51 
                 5161 
                 5176 
                 1597 
               
               
                   
               
               
                 826878 
                 N/A 
                 N/A 
                 TTGTCCTAGCACCTCC 
                 25 
                 4870 
                 4885 
                 1598 
               
               
                   
               
               
                 826879 
                 N/A 
                 N/A 
                 GTTTTGTCCTAGCACC 
                 21 
                 4873 
                 4888 
                 1599 
               
               
                   
               
               
                 826888 
                 N/A 
                 N/A 
                 GATAGGGCCACCTTTC 
                 31 
                 4891 
                 4906 
                 1600 
               
               
                   
               
               
                 826889 
                 N/A 
                 N/A 
                 CTGATAGGGCCACCTT 
                 38 
                 4893 
                 4908 
                 1601 
               
               
                   
               
               
                 826890 
                 N/A 
                 N/A 
                 TCCCTGATAGGGCCAC 
                 24 
                 4896 
                 4911 
                 1602 
               
               
                   
               
               
                 826891 
                 N/A 
                 N/A 
                 CTTCCCTGATAGGGCC 
                 15 
                 4898 
                 4913 
                 1603 
               
               
                   
               
               
                 826892 
                 N/A 
                 N/A 
                 TGCTTCCCTGATAGGG 
                 32 
                 4900 
                 4915 
                 1604 
               
               
                   
               
               
                 826893 
                 N/A 
                 N/A 
                 AACGGCCTCTCCTCTG 
                 18 
                 4914 
                 4929 
                 1605 
               
               
                   
               
               
                 826894 
                 N/A 
                 N/A 
                 GAACGGCCTCTCCTCT 
                 27 
                 4915 
                 4930 
                 1606 
               
               
                   
               
               
                 826895 
                 N/A 
                 N/A 
                 TAGAACGGCCTCTCCT 
                 50 
                 4917 
                 4932 
                 1607 
               
               
                   
               
               
                 826898 
                 N/A 
                 N/A 
                 GGCTTCCCTAGAACGG 
                 44 
                 4925 
                 4940 
                 1608 
               
               
                   
               
               
                 826899 
                 N/A 
                 N/A 
                 CTGGGCTTCCCTAGAA 
                 47 
                 4928 
                 4943 
                 1609 
               
               
                   
               
               
                 826908 
                 N/A 
                 N/A 
                 GGGCCAAAAGTGCCGG 
                 49 
                 4946 
                 4961 
                 1610 
               
               
                   
               
               
                 826909 
                 N/A 
                 N/A 
                 GACCTGCGGGAGTTGG 
                 42 
                 4961 
                 4976 
                 1611 
               
               
                   
               
               
                 826910 
                 N/A 
                 N/A 
                 CAGACCTGCGGGAGTT 
                 18 
                 4963 
                 4978 
                 1612 
               
               
                   
               
               
                 826911 
                 N/A 
                 N/A 
                 GCAGACCTGCGGGAGT 
                 26 
                 4964 
                 4979 
                 1613 
               
               
                   
               
               
                 826912 
                 N/A 
                 N/A 
                 GCGCCCACATTCTCCC 
                 48 
                 5015 
                 5030 
                 1614 
               
               
                   
               
               
                 826913 
                 N/A 
                 N/A 
                 CCTGCGCCCACATTCT 
                 55 
                 5018 
                 5033 
                 1615 
               
               
                   
               
               
                 826914 
                 N/A 
                 N/A 
                 CCCTGCGCCCACATTC 
                 68 
                 5019 
                 5034 
                 1616 
               
               
                   
               
               
                 826915 
                 N/A 
                 N/A 
                 CCACCCTGCGCCCACA 
                 48 
                 5022 
                 5037 
                 1617 
               
               
                   
               
               
                 826918 
                 N/A 
                 N/A 
                 GGAACCCGAGTGAGGC 
                 30 
                 5060 
                 5075 
                 1618 
               
               
                   
               
               
                 826919 
                 N/A 
                 N/A 
                 TGGAACCCGAGTGAGG 
                 41 
                 5061 
                 5076 
                 1619 
               
               
                   
               
               
                 826928 
                 N/A 
                 N/A 
                 CCCTTCATGAGCCCCG 
                 23 
                 5150 
                 5165 
                 1620 
               
               
                   
               
               
                 826929 
                 N/A 
                 N/A 
                 CCCCTTCATGAGCCCC 
                 42 
                 5151 
                 5166 
                 1621 
               
               
                   
               
               
                 826930 
                 N/A 
                 N/A 
                 AGCTTGTTCCCCTTCA 
                 23 
                 5159 
                 5174 
                 1622 
               
               
                   
               
               
                 826931 
                 N/A 
                 N/A 
                 CAGCTTGTTCCCCTTC 
                 35 
                 5160 
                 5175 
                 1623 
               
               
                   
               
               
                 826932 
                 N/A 
                 N/A 
                 CTGCAATAAGGTGCTC 
                 106 
                 2302 
                 2317 
                 1624 
               
               
                   
               
               
                 826933 
                 N/A 
                 N/A 
                 GGGCATGGTCCTCCCT 
                 54 
                 2316 
                 2331 
                 1625 
               
               
                   
               
               
                 826934 
                 N/A 
                 N/A 
                 GTCCTTACATTGGGCA 
                 71 
                 2327 
                 2342 
                 1626 
               
               
                   
               
               
                 826935 
                 N/A 
                 N/A 
                 CCTAGAAACTCCAGTC 
                 75 
                 2356 
                 2371 
                 1627 
               
               
                   
               
               
                 826938 
                 N/A 
                 N/A 
                 GGCTATCTACTTAGCG 
                 86 
                 2568 
                 2583 
                 1628 
               
               
                   
               
               
                 826939 
                 N/A 
                 N/A 
                 ATAGGAGCAGAGCTAT 
                 101 
                 2616 
                 2631 
                 1629 
               
               
                   
               
               
                 826948 
                 N/A 
                 N/A 
                 GTGCAGATCTCAGATT 
                 58 
                 3013 
                 3028 
                 1630 
               
               
                   
               
               
                 826949 
                 N/A 
                 N/A 
                 ACGGACTTCTAACAAA 
                 73 
                 3030 
                 3045 
                 1631 
               
               
                   
               
               
                 826950 
                 N/A 
                 N/A 
                 AGGAGATAGGCCTGCA 
                 73 
                 3097 
                 3112 
                 1632 
               
               
                   
               
               
                 826951 
                 N/A 
                 N/A 
                 ATTGATACACACCGGG 
                 59 
                 3115 
                 3130 
                 1633 
               
               
                   
               
               
                 826952 
                 N/A 
                 N/A 
                 GTGCAGGAATGTGGTC 
                 90 
                 3185 
                 3200 
                 1634 
               
               
                   
               
               
                 826953 
                 N/A 
                 N/A 
                 GGGAAGGCTGCCGCTT 
                 41 
                 3231 
                 3246 
                 1635 
               
               
                   
               
               
                 826954 
                 N/A 
                 N/A 
                 CTGCACGCGGCAGGGA 
                 62 
                 3243 
                 3258 
                 1636 
               
               
                   
               
               
                 826955 
                 N/A 
                 N/A 
                 AGGAGACTCGGGAGAG 
                 90 
                 3279 
                 3294 
                 1637 
               
               
                   
               
               
                 826958 
                 N/A 
                 N/A 
                 AAGGAGTGGAGTGCCA 
                 90 
                 3350 
                 3365 
                 1638 
               
               
                   
               
               
                 826959 
                 N/A 
                 N/A 
                 CCAAACTTAATGCAGC 
                 54 
                 3374 
                 3389 
                 1639 
               
               
                   
               
               
                 826968 
                 N/A 
                 N/A 
                 CCAAAGGGAGTCTGTC 
                 59 
                 3981 
                 3996 
                 1640 
               
               
                   
               
               
                 826969 
                 N/A 
                 N/A 
                 GCAAATAGAAGGAGCC 
                 76 
                 4001 
                 4016 
                 1641 
               
               
                   
               
               
                 826970 
                 N/A 
                 N/A 
                 ACTGAGTGAGTAGAGG 
                 67 
                 4039 
                 4054 
                 1642 
               
               
                   
               
               
                 826971 
                 N/A 
                 N/A 
                 GGGACAGCGAAGGACA 
                 54 
                 4079 
                 4094 
                 1643 
               
               
                   
               
               
                 826972 
                 N/A 
                 N/A 
                 ACAATAGAGAGGGACA 
                 99 
                 4089 
                 4104 
                 1644 
               
               
                   
               
               
                 826973 
                 N/A 
                 N/A 
                 GCCCACAGCTAGGAGG 
                 66 
                 4185 
                 4200 
                 1645 
               
               
                   
               
               
                 826974 
                 N/A 
                 N/A 
                 AGTAGAAGGATCCTGA 
                 50 
                 4201 
                 4216 
                 1646 
               
               
                   
               
               
                 826975 
                 N/A 
                 N/A 
                 TGGCAGCCAAACCTCT 
                 69 
                 4509 
                 4524 
                 1647 
               
               
                   
               
               
                 826978 
                 N/A 
                 N/A 
                 TCCCAGGTTGCGGCTG 
                 42 
                 4555 
                 4570 
                 1648 
               
               
                   
               
               
                 826979 
                 N/A 
                 N/A 
                 CCTGACCTCGAGCTGT 
                 38 
                 4639 
                 4654 
                 1649 
               
               
                   
               
               
                 826988 
                 N/A 
                 N/A 
                 CTTATACTTTGCTGGC 
                 25 
                 5994 
                 6009 
                 1650 
               
               
                   
               
               
                 826989 
                 N/A 
                 N/A 
                 GGTGGACGAGGTCTTA 
                 27 
                 6006 
                 6021 
                 1651 
               
               
                   
               
               
                 826990 
                 N/A 
                 N/A 
                 CTGCACGGTCTCGCCT 
                 43 
                 6064 
                 6079 
                 1652 
               
               
                   
               
               
                 826991 
                 N/A 
                 N/A 
                 CCCTAACCTCCACGAT 
                 73 
                 6150 
                 6165 
                 1653 
               
               
                   
               
               
                 826992 
                 N/A 
                 N/A 
                 CTGTAAGGCCCCTGCC 
                 43 
                 6190 
                 6205 
                 1654 
               
               
                   
               
               
                 826993 
                 N/A 
                 N/A 
                 GGGCAGGTCAACAGTG 
                 30 
                 6282 
                 6297 
                 1655 
               
               
                   
               
               
                 826994 
                 N/A 
                 N/A 
                 GTAAAGGTCAGGCACC 
                 42 
                 6299 
                 6314 
                 1656 
               
               
                   
               
               
                 826995 
                 N/A 
                 N/A 
                 CCGATGAAACCCAAAA 
                 59 
                 6336 
                 6351 
                 1657 
               
               
                   
               
               
                 826998 
                 N/A 
                 N/A 
                 CGCTTACCACCTGCTC 
                 38 
                 6383 
                 6398 
                 1658 
               
               
                   
               
               
                 826999 
                 N/A 
                 N/A 
                 AGGTATACAAAAGCAC 
                 98 
                 6425 
                 6440 
                 1659 
               
               
                   
               
               
                 827008 
                 N/A 
                 N/A 
                 GTACACTAACTCACCA 
                 53 
                 6657 
                 6672 
                 1660 
               
               
                   
               
               
                 827009 
                 N/A 
                 N/A 
                 AAAGATTTTGCACTCC 
                 42 
                 6679 
                 6694 
                 1661 
               
               
                   
               
               
                 827010 
                 N/A 
                 N/A 
                 ACCCACACCCATAAAG 
                 98 
                 6691 
                 6706 
                 1662 
               
               
                   
               
               
                 827011 
                 N/A 
                 N/A 
                 TACCAATATGTGCACA 
                 44 
                 6718 
                 6733 
                 1663 
               
               
                   
               
               
                 827012 
                 N/A 
                 N/A 
                 TCGCACACATACCAAT 
                 78 
                 6727 
                 6742 
                 1664 
               
               
                   
               
               
                 827013 
                 N/A 
                 N/A 
                 CAGCATCCAAAATCGC 
                 44 
                 6739 
                 6754 
                 1665 
               
               
                   
               
               
                 827014 
                 N/A 
                 N/A 
                 ACCCACAGCATGACCA 
                 68 
                 6760 
                 6775 
                 1666 
               
               
                   
               
               
                 827015 
                 N/A 
                 N/A 
                 ATGGAATATACGAAGG 
                 35 
                 6783 
                 6798 
                 1667 
               
               
                   
               
               
                 827018 
                 N/A 
                 N/A 
                 ACGAAATGACCTGGCT 
                 35 
                 6870 
                 6885 
                 1668 
               
               
                   
               
               
                 827019 
                 N/A 
                 N/A 
                 GGACATTATACAGACG 
                 30 
                 6893 
                 6908 
                 1669 
               
               
                   
               
               
                 827028 
                 N/A 
                 N/A 
                 CACCAAGGCCTAAAGG 
                 62 
                 7268 
                 7283 
                 1670 
               
               
                   
               
               
                 827029 
                 N/A 
                 N/A 
                 GGCCTCACCCGATTCA 
                 47 
                 7395 
                 7410 
                 1671 
               
               
                   
               
               
                 827030 
                 N/A 
                 N/A 
                 TAAGAACTGTGCAGGC 
                 5 
                 7408 
                 7423 
                 1672 
               
               
                   
               
               
                 827031 
                 N/A 
                 N/A 
                 GTCTAGGGCCCCGCAT 
                 47 
                 7435 
                 7450 
                 1673 
               
               
                   
               
               
                 827032 
                 N/A 
                 N/A 
                 GGGCTTATGGCTTCCT 
                 42 
                 7473 
                 7488 
                 1674 
               
               
                   
               
               
                 827033 
                 N/A 
                 N/A 
                 CTCCTAGGTGTTCTCT 
                 38 
                 7576 
                 7591 
                 1675 
               
               
                   
               
               
                 827034 
                 N/A 
                 N/A 
                 TGCTTGGTGGGTGTTG 
                 53 
                 7638 
                 7653 
                 1676 
               
               
                   
               
               
                 827035 
                 N/A 
                 N/A 
                 GTCAAGTGTGTAGTGC 
                 14 
                 7651 
                 7666 
                 1677 
               
               
                   
               
               
                 827038 
                 N/A 
                 N/A 
                 GCTTTAGGGTGTAGCA 
                 76 
                 7684 
                 7699 
                 1678 
               
               
                   
               
               
                 827039 
                 N/A 
                 N/A 
                 GTCTATAAAGCACCCA 
                 36 
                 7700 
                 7715 
                 1679 
               
               
                   
               
               
                 827048 
                 N/A 
                 N/A 
                 GCTAACCTGAGATGCC 
                 64 
                 8501 
                 8516 
                 1680 
               
               
                   
               
               
                 827049 
                 N/A 
                 N/A 
                 CACTAGGTTTGTGACT 
                 82 
                 8522 
                 8537 
                 1681 
               
               
                   
               
               
                 827050 
                 N/A 
                 N/A 
                 AATCAAACACACTAGG 
                 46 
                 8531 
                 8546 
                 1682 
               
               
                   
               
               
                 827051 
                 N/A 
                 N/A 
                 GGGTAAAAGAGCTTTG 
                 25 
                 8548 
                 8563 
                 1683 
               
               
                   
               
               
                 827052 
                 N/A 
                 N/A 
                 CCCTACTTAAAGTGTA 
                 83 
                 8566 
                 8581 
                 1684 
               
               
                   
               
               
                 827053 
                 N/A 
                 N/A 
                 CCTGACAAATTGTCCT 
                 26 
                 8651 
                 8666 
                 1685 
               
               
                   
               
               
                 827054 
                 N/A 
                 N/A 
                 TTAACCTGTTTACCTC 
                 38 
                 8717 
                 8732 
                 1686 
               
               
                   
               
               
                 827055 
                 N/A 
                 N/A 
                 TCCCGGTGATTCACTC 
                 61 
                 8744 
                 8759 
                 1687 
               
               
                   
               
               
                 827058 
                 N/A 
                 N/A 
                 AACCGATCTCCTCGGT 
                 110 
                 8778 
                 8793 
                 1688 
               
               
                   
               
               
                 827059 
                 N/A 
                 N/A 
                 CTCTAGCTCATCAACC 
                 69 
                 8790 
                 8805 
                 1689 
               
               
                   
               
               
                 827068 
                 N/A 
                 N/A 
                 CCCTAGCTCAGGGCTT 
                 48 
                 9259 
                 9274 
                 1690 
               
               
                   
               
               
                 827069 
                 N/A 
                 N/A 
                 ACAGGCAGGGACGGCC 
                 32 
                 9276 
                 9291 
                 1691 
               
               
                   
               
               
                 827070 
                 N/A 
                 N/A 
                 ATGCAGGGTCTGCCCG 
                 42 
                 9307 
                 9322 
                 1692 
               
               
                   
               
               
                 827071 
                 N/A 
                 N/A 
                 CTCAACAGTCCAGGCT 
                 29 
                 9376 
                 9391 
                 1693 
               
               
                   
               
               
                 827072 
                 N/A 
                 N/A 
                 GGTTAGAGGGATGTCA 
                 48 
                 9395 
                 9410 
                 1694 
               
               
                   
               
               
                 827073 
                 N/A 
                 N/A 
                 CACCATGGCAGGGTTA 
                 45 
                 9406 
                 9421 
                 1695 
               
               
                   
               
               
                 827074 
                 N/A 
                 N/A 
                 AGCCGCCTAGGCCCCA 
                 40 
                 9449 
                 9464 
                 1696 
               
               
                   
               
               
                 827075 
                 N/A 
                 N/A 
                 GCCCATGCTCATCCTA 
                 62 
                 9476 
                 9491 
                 1697 
               
               
                   
               
               
                 827078 
                 N/A 
                 N/A 
                 CCAGGACGGAGCAGCA 
                 60 
                 9585 
                 9600 
                 1698 
               
               
                   
               
               
                 827079 
                 N/A 
                 N/A 
                 AACTAGGCAAATTCCC 
                 41 
                 9775 
                 9790 
                 1699 
               
               
                   
               
               
                 827088 
                 N/A 
                 N/A 
                 TAGAAATTCCTATAGC 
                 46 
                 10104 
                 10119 
                 1700 
               
               
                   
               
               
                 827089 
                 N/A 
                 N/A 
                 CATGACCCCGTGATAC 
                 50 
                 10120 
                 10135 
                 1701 
               
               
                   
               
               
                 827090 
                 N/A 
                 N/A 
                 TATGATAAATTAGCCG 
                 57 
                 10310 
                 10325 
                 1702 
               
               
                   
               
               
                 827091 
                 N/A 
                 N/A 
                 GACGGAATGGCCGGGC 
                 24 
                 10443 
                 10458 
                 1703 
               
               
                   
               
               
                 827092 
                 N/A 
                 N/A 
                 TGGCATAAGATAAGAC 
                 34 
                 10456 
                 10471 
                 1704 
               
               
                   
               
               
                 827093 
                 N/A 
                 N/A 
                 CCAAAAGGTCTACTGC 
                 30 
                 10482 
                 10497 
                 1705 
               
               
                   
               
               
                 827094 
                 N/A 
                 N/A 
                 TTCCATAGGGCCCCAC 
                 50 
                 10508 
                 10523 
                 1706 
               
               
                   
               
               
                 827095 
                 N/A 
                 N/A 
                 CACTGATGAGCCCCCC 
                 82 
                 10601 
                 10616 
                 1707 
               
               
                   
               
               
                 827098 
                 N/A 
                 N/A 
                 CCTGCCACCCTACGCG 
                 44 
                 10636 
                 10651 
                 1708 
               
               
                   
               
               
                 827099 
                 N/A 
                 N/A 
                 TCCTGCCACCCTACGC 
                 46 
                 10637 
                 10652 
                 1709 
               
               
                   
                   
                   
                   
                   
                 10660 
                 10675 
                   
               
               
                   
                   
                   
                   
                   
                 10683 
                 10698 
                   
               
               
                   
               
               
                 827108 
                 N/A 
                 N/A 
                 CCCTACACGCCTCCCT 
                 56 
                 10721 
                 10736 
                 1710 
               
               
                   
               
               
                 827109 
                 N/A 
                 N/A 
                 TCAATCTGGTTGTCAC 
                 55 
                 10812 
                 10827 
                 1711 
               
               
                   
               
               
                 827110 
                 N/A 
                 N/A 
                 TCTCATCACCAACTTC 
                 42 
                 10826 
                 10841 
                 1712 
               
               
                   
               
               
                 827111 
                 N/A 
                 N/A 
                 AAGAATCCAGATCCCC 
                 36 
                 10943 
                 10958 
                 1713 
               
               
                   
               
               
                 827112 
                 N/A 
                 N/A 
                 AGCGAATTTGCCTTTC 
                 34 
                 10974 
                 10989 
                 1714 
               
               
                   
               
               
                 827113 
                 N/A 
                 N/A 
                 GCCCAGGAAAGCGAAT 
                 55 
                 10983 
                 10998 
                 1715 
               
               
                   
               
               
                 827114 
                 N/A 
                 N/A 
                 TCGACTATCAGGAAGA 
                 42 
                 11015 
                 11030 
                 1716 
               
               
                   
               
               
                 827115 
                 N/A 
                 N/A 
                 GTGAAGAGACCATCGA 
                 41 
                 11027 
                 11042 
                 1717 
               
               
                   
               
               
                 827118 
                 N/A 
                 N/A 
                 GGGTAAGTGACCCAGC 
                 48 
                 11154 
                 11169 
                 1718 
               
               
                   
               
               
                 827119 
                 N/A 
                 N/A 
                 GAAAGAGCACCCAAGC 
                 51 
                 11233 
                 11248 
                 1719 
               
               
                   
               
               
                 827128 
                 N/A 
                 N/A 
                 TACCGTTAGCCACTGT 
                 30 
                 11418 
                 11433 
                 1720 
               
               
                   
               
               
                 827129 
                 N/A 
                 N/A 
                 CTTCAGTGTAACACAG 
                 41 
                 11436 
                 11451 
                 1721 
               
               
                   
               
               
                 827130 
                 N/A 
                 N/A 
                 CTTTAGGACAAACTTT 
                 62 
                 11503 
                 11518 
                 1722 
               
               
                   
               
               
                 827131 
                 N/A 
                 N/A 
                 TCACTATGCATGAAGA 
                 14 
                 11522 
                 11537 
                 1723 
               
               
                   
               
               
                 827132 
                 N/A 
                 N/A 
                 ATCTAGTTAGGTGGCA 
                 32 
                 11540 
                 11555 
                 1724 
               
               
                   
               
               
                 827133 
                 N/A 
                 N/A 
                 AGGTAGGTTATAGTGT 
                 22 
                 11564 
                 11579 
                 1725 
               
               
                   
               
               
                 827134 
                 N/A 
                 N/A 
                 TGCTATAAAGGTAGGT 
                 18 
                 11572 
                 11587 
                 1726 
               
               
                   
               
               
                 827135 
                 N/A 
                 N/A 
                 TGACAAGTGGGCTGCC 
                 44 
                 11612 
                 11627 
                 1727 
               
               
                   
               
               
                 827138 
                 N/A 
                 N/A 
                 GTACAGAAACACCCGG 
                 63 
                 11669 
                 11684 
                 1728 
               
               
                   
               
               
                 827139 
                 N/A 
                 N/A 
                 AACGGAAGTAAGGTAC 
                 47 
                 11681 
                 11696 
                 1729 
               
               
                   
               
               
                 827148 
                 N/A 
                 N/A 
                 CGTTTATCGAGCACTT 
                 13 
                 11915 
                 11930 
                 1730 
               
               
                   
               
               
                 827149 
                 N/A 
                 N/A 
                 GGCAAGCATAGCTAGC 
                 18 
                 11930 
                 11945 
                 1731 
               
               
                   
               
               
                 827150 
                 N/A 
                 N/A 
                 GTGTGTTTGGCATTCT 
                 7 
                 11980 
                 11995 
                 1732 
               
               
                   
                   
                   
                   
                   
                 13086 
                 13101 
                   
               
               
                   
               
               
                 827151 
                 N/A 
                 N/A 
                 GGTGTGTTTGGCATTC 
                 12 
                 11981 
                 11996 
                 1733 
               
               
                   
               
               
                 827152 
                 N/A 
                 N/A 
                 AGGTGTGTTTGGCATT 
                 29 
                 11982 
                 11997 
                 1734 
               
               
                   
               
               
                 827153 
                 N/A 
                 N/A 
                 GCCTTAGGCATCAGCT 
                 25 
                 12045 
                 12060 
                 1735 
               
               
                   
               
               
                 827154 
                 N/A 
                 N/A 
                 GTCTAGCTGGCTGGGC 
                 42 
                 12059 
                 12074 
                 1736 
               
               
                   
               
               
                 827155 
                 N/A 
                 N/A 
                 AACCACCGTCTAGTCC 
                 42 
                 12126 
                 12141 
                 1737 
               
               
                   
               
               
                 827158 
                 N/A 
                 N/A 
                 CCAGAACAAGGTTGTT 
                 56 
                 12181 
                 12196 
                 1738 
               
               
                   
               
               
                 827159 
                 N/A 
                 N/A 
                 TCAGATTTAATGGGTC 
                 43 
                 12207 
                 12222 
                 1739 
               
               
                   
               
               
                 827168 
                 N/A 
                 N/A 
                 AACCAGTTGATAGAGA 
                 44 
                 12409 
                 12424 
                 1740 
               
               
                   
               
               
                 827169 
                 N/A 
                 N/A 
                 ATACGAATTCTATGAA 
                 80 
                 12432 
                 12447 
                 1741 
               
               
                   
               
               
                 827170 
                 N/A 
                 N/A 
                 TCCCATTTATACGAAT 
                 57 
                 12440 
                 12455 
                 1742 
               
               
                   
               
               
                 827171 
                 N/A 
                 N/A 
                 CCAGAATAGGCTCATC 
                 33 
                 12570 
                 12585 
                 1743 
               
               
                   
               
               
                 827172 
                 N/A 
                 N/A 
                 GCTCAAATCAGGCAGC 
                 89 
                 12593 
                 12608 
                 1744 
               
               
                   
               
               
                 827173 
                 N/A 
                 N/A 
                 GCAAAGAACGATGCTC 
                 46 
                 12605 
                 12620 
                 1745 
               
               
                   
               
               
                 827174 
                 N/A 
                 N/A 
                 TAACAGAGTTGACTTG 
                 100 
                 12622 
                 12637 
                 1746 
               
               
                   
               
               
                 827175 
                 N/A 
                 N/A 
                 TGGTATTAGAATGTGC 
                 18 
                 12681 
                 12696 
                 1747 
               
               
                   
               
               
                 827178 
                 N/A 
                 N/A 
                 ACGACGAAACCTTGTA 
                 48 
                 12932 
                 12947 
                 1748 
               
               
                   
               
               
                 827179 
                 N/A 
                 N/A 
                 GTGTTTGGCATTCTAG 
                 19 
                 13084 
                 13099 
                 1749 
               
               
                   
               
               
                 827188 
                 N/A 
                 N/A 
                 ACCATATAACCCATCC 
                 30 
                 13715 
                 13730 
                 1750 
               
               
                   
               
               
                 827189 
                 N/A 
                 N/A 
                 ATGATACGATCATTTT 
                 34 
                 13774 
                 13789 
                 1751 
               
               
                   
               
               
                 827190 
                 N/A 
                 N/A 
                 CTGTACACAGCTAGTG 
                 30 
                 13800 
                 13815 
                 1752 
               
               
                   
               
               
                 827191 
                 N/A 
                 N/A 
                 CGAACAGACCTACATT 
                 41 
                 13833 
                 13848 
                 1753 
               
               
                   
               
               
                 827192 
                 N/A 
                 N/A 
                 CCGAACAGACCTACAT 
                 32 
                 13834 
                 13849 
                 1754 
               
               
                   
               
               
                 827193 
                 N/A 
                 N/A 
                 AGCCGAACAGACCTAC 
                 42 
                 13836 
                 13851 
                 1755 
               
               
                   
               
               
                 827194 
                 N/A 
                 N/A 
                 TGAACAGACCTACATT 
                 52 
                 14130 
                 14145 
                 1756 
               
               
                   
               
               
                 827195 
                 N/A 
                 N/A 
                 ATGAACAGACCTACAT 
                 60 
                 14131 
                 14146 
                 1757 
               
               
                   
               
               
                 827198 
                 N/A 
                 N/A 
                 AGTAGGCACTTTATGA 
                 55 
                 14143 
                 14158 
                 1758 
               
               
                   
               
               
                 827199 
                 N/A 
                 N/A 
                 CCGTATGTAGTAGGCA 
                 24 
                 14151 
                 14166 
                 1759 
               
               
                   
               
               
                 827207 
                 N/A 
                 N/A 
                 CTAGAACAACCGTATG 
                 29 
                 14160 
                 14175 
                 1760 
               
               
                   
               
               
                 827208 
                 N/A 
                 N/A 
                 CCTAGAACAACCGTAT 
                 34 
                 14161 
                 14176 
                 1761 
               
               
                   
               
               
                 827209 
                 N/A 
                 N/A 
                 CCCTAGAACAACCGTA 
                 30 
                 14162 
                 14177 
                 1762 
               
               
                   
               
               
                 827210 
                 N/A 
                 N/A 
                 TCCCTAGAACAACCGT 
                 21 
                 14163 
                 14178 
                 1763 
               
               
                   
               
               
                 827211 
                 N/A 
                 N/A 
                 TGGAAGATATCTTCCT 
                 90 
                 14229 
                 14244 
                 1764 
               
               
                   
               
               
                 827212 
                 N/A 
                 N/A 
                 CCTTATGCTATACAGG 
                 43 
                 14311 
                 14326 
                 1765 
               
               
                   
               
               
                 827213 
                 N/A 
                 N/A 
                 GAATACTGTATTGGAA 
                 43 
                 14349 
                 14364 
                 1766 
               
               
                   
               
               
                 827214 
                 N/A 
                 N/A 
                 TGTTAGCAGGTTCTGC 
                 48 
                 14375 
                 14390 
                 1767 
               
               
                   
               
               
                 827217 
                 N/A 
                 N/A 
                 ACAATGCGGTTCTTGG 
                 38 
                 14507 
                 14522 
                 1768 
               
               
                   
               
               
                 827218 
                 N/A 
                 N/A 
                 CTAAGACTTATCTGGA 
                 48 
                 14629 
                 14644 
                 1769 
               
               
                   
               
               
                 827227 
                 N/A 
                 N/A 
                 TGCATTTAGGCCGGGT 
                 46 
                 15520 
                 15535 
                 1770 
               
               
                   
               
               
                 827228 
                 N/A 
                 N/A 
                 TTGCAGGGTACACAAC 
                 51 
                 15557 
                 15572 
                 1771 
               
               
                   
               
               
                 827229 
                 N/A 
                 N/A 
                 CTGAACAAGGTTGCAG 
                 46 
                 15567 
                 15582 
                 1772 
               
               
                   
               
               
                 827230 
                 N/A 
                 N/A 
                 TAGAACTAACAAACTG 
                 53 
                 15580 
                 15595 
                 1773 
               
               
                   
               
               
                 827231 
                 N/A 
                 N/A 
                 GGCCTGAGGGATGTCA 
                 52 
                 15617 
                 15632 
                 1774 
               
               
                   
               
               
                 827232 
                 N/A 
                 N/A 
                 CATCATGAAAGTCCAG 
                 39 
                 15641 
                 15656 
                 1775 
               
               
                   
               
               
                 827233 
                 N/A 
                 N/A 
                 CACCGAAATCAAGAGT 
                 58 
                 15834 
                 15849 
                 1776 
               
               
                   
               
               
                 827234 
                 N/A 
                 N/A 
                 TGCCGCTTGGCACCGA 
                 96 
                 15844 
                 15859 
                 1777 
               
               
                   
               
               
                 827237 
                 N/A 
                 N/A 
                 ACCCAGGTCATCCCGC 
                 106 
                 15902 
                 15917 
                 1778 
               
               
                   
               
               
                 827238 
                 N/A 
                 N/A 
                 ACCCGGAACTTGTCTG 
                 45 
                 15971 
                 15986 
                 1779 
               
               
                   
               
               
                 827247 
                 N/A 
                 N/A 
                 CCCAAAAGCTTGGGCA 
                 40 
                 16752 
                 16767 
                 1780 
               
               
                   
               
               
                 827248 
                 N/A 
                 N/A 
                 ACATAGGACCCCAGGG 
                 37 
                 16775 
                 16790 
                 1781 
               
               
                   
               
               
                 827249 
                 N/A 
                 N/A 
                 TCCCACTAGTGGGCAC 
                 53 
                 16919 
                 16934 
                 1782 
               
               
                   
               
               
                 827250 
                 N/A 
                 N/A 
                 TCCTAACTGAGTCCCA 
                 32 
                 16930 
                 16945 
                 1783 
               
               
                   
               
               
                 827251 
                 N/A 
                 N/A 
                 TCACGCTGGAGGGTCC 
                 31 
                 16943 
                 16958 
                 1784 
               
               
                   
               
               
                 827252 
                 N/A 
                 N/A 
                 TGTTAGCCCAGTTCTC 
                 44 
                 16961 
                 16976 
                 1785 
               
               
                   
               
               
                 827253 
                 N/A 
                 N/A 
                 CTATCTTGGGCTGTTA 
                 93 
                 16972 
                 16987 
                 1786 
               
               
                   
               
               
                 827254 
                 N/A 
                 N/A 
                 GGCAGACGAGCTCACT 
                 11 
                 17288 
                 17303 
                 1787 
               
               
                   
               
               
                 827257 
                 N/A 
                 N/A 
                 GACTGAGGGATCAAGA 
                 44 
                 17431 
                 17446 
                 1788 
               
               
                   
               
               
                 827258 
                 N/A 
                 N/A 
                 TGCCTAGGGTGGAAGG 
                 42 
                 17481 
                 17496 
                 1789 
               
               
                   
               
               
                 827267 
                 N/A 
                 N/A 
                 GACTAGAGTCAGAGGG 
                 43 
                 17733 
                 17748 
                 1790 
               
               
                   
               
               
                 827268 
                 N/A 
                 N/A 
                 TCTGGTTGCACTGGAC 
                 28 
                 17754 
                 17769 
                 1791 
               
               
                   
               
               
                 827269 
                 N/A 
                 N/A 
                 TTCTGGTTGCACTGGA 
                 38 
                 17755 
                 17770 
                 1792 
               
               
                   
               
               
                 827270 
                 N/A 
                 N/A 
                 GTTCTGGTTGCACTGG 
                 15 
                 17756 
                 17771 
                 1793 
               
               
                   
               
               
                 827271 
                 N/A 
                 N/A 
                 TGTTCTGGTTGCACTG 
                 37 
                 17757 
                 17772 
                 1794 
               
               
                   
               
               
                 827272 
                 N/A 
                 N/A 
                 TTGTTCTGGTTGCACT 
                 32 
                 17758 
                 17773 
                 1795 
               
               
                   
               
               
                 827273 
                 N/A 
                 N/A 
                 GCGGACCCCGCGGAGA 
                 39 
                 17978 
                 17993 
                 1796 
               
               
                   
               
               
                 827274 
                 N/A 
                 N/A 
                 ACCCAGGGAAGCGGAC 
                 60 
                 17988 
                 18003 
                 1797 
               
               
                   
               
               
                 827277 
                 N/A 
                 N/A 
                 ATCCATGCTTCCAGCC 
                 14 
                 18214 
                 18229 
                 1798 
               
               
                   
               
               
                 827278 
                 N/A 
                 N/A 
                 GATCCATGCTTCCAGC 
                 19 
                 18215 
                 18230 
                 1799 
               
               
                   
               
               
                 827286 
                 N/A 
                 N/A 
                 AAAGACCAAGATCCAT 
                 19 
                 18224 
                 18239 
                 1800 
               
               
                   
               
               
                 827287 
                 N/A 
                 N/A 
                 GGCCTTAGAAAGACCA 
                 86 
                 18551 
                 18566 
                 1801 
               
               
                   
               
               
                 827288 
                 N/A 
                 N/A 
                 AGCTTTGATGCTAGGG 
                 16 
                 18574 
                 18589 
                 1802 
               
               
                   
               
               
                 827289 
                 N/A 
                 N/A 
                 GACAGATGATCTCCTA 
                 11 
                 18600 
                 18615 
                 1803 
               
               
                   
               
               
                 827290 
                 N/A 
                 N/A 
                 CCTCACTACTACTGCC 
                 21 
                 18643 
                 18658 
                 1804 
               
               
                   
               
               
                 827291 
                 N/A 
                 N/A 
                 CCTCAACCCATGCCAC 
                 51 
                 18663 
                 18678 
                 1805 
               
               
                   
               
               
                 827292 
                 N/A 
                 N/A 
                 ACTTAGGTTTAGTCCC 
                 36 
                 18677 
                 18692 
                 1806 
               
               
                   
               
               
                 827293 
                 N/A 
                 N/A 
                 AGCTAGAGTGGGAACT 
                 44 
                 18690 
                 18705 
                 1807 
               
               
                   
               
               
                 827296 
                 N/A 
                 N/A 
                 TGATACATCCAGAGTC 
                 42 
                 18726 
                 18741 
                 1808 
               
               
                   
               
               
                 827297 
                 N/A 
                 N/A 
                 ATGATGTGATACATCC 
                 58 
                 18732 
                 18747 
                 1809 
               
               
                   
               
               
                 827305 
                 N/A 
                 N/A 
                 ACACACTTGGTACAGC 
                 23 
                 18964 
                 18979 
                 1810 
               
               
                   
               
               
                 827306 
                 N/A 
                 N/A 
                 GGTCTATAAAGTGCCC 
                 23 
                 18995 
                 19010 
                 1811 
               
               
                   
               
               
                 827307 
                 N/A 
                 N/A 
                 AGAGTAATGAAACCCA 
                 5 
                 19022 
                 19037 
                 1812 
               
               
                   
               
               
                 827308 
                 N/A 
                 N/A 
                 CTTCACCTGTTTGAGT 
                 32 
                 19041 
                 19056 
                 1813 
               
               
                   
               
               
                 827309 
                 N/A 
                 N/A 
                 TCCTTAGCCAGGGCCG 
                 15 
                 19079 
                 19094 
                 1814 
               
               
                   
               
               
                 827310 
                 N/A 
                 N/A 
                 AATGAATACCCGAGGG 
                 25 
                 19113 
                 19128 
                 1815 
               
               
                   
               
               
                 827311 
                 N/A 
                 N/A 
                 GGACATTATAACAGGG 
                 28 
                 19139 
                 19154 
                 1816 
               
               
                   
               
               
                 827312 
                 N/A 
                 N/A 
                 CTGCTATGAGCTGCTT 
                 71 
                 19159 
                 19174 
                 1817 
               
               
                   
               
               
                 827315 
                 N/A 
                 N/A 
                 CTGTAGAGTGGAGCCA 
                 44 
                 19305 
                 19320 
                 1818 
               
               
                   
               
               
                 827316 
                 N/A 
                 N/A 
                 AGGGAATGCCCCCTGT 
                 95 
                 19317 
                 19332 
                 1819 
               
               
                   
               
               
                 827325 
                 N/A 
                 N/A 
                 GGCATAGGGAAAGCAC 
                 30 
                 19542 
                 19557 
                 1820 
               
               
                   
                   
                   
                   
                   
                 19624 
                 19639 
                   
               
               
                   
               
               
                 827326 
                 N/A 
                 N/A 
                 GAGGCATCGGGTGAGG 
                 47 
                 19557 
                 19572 
                 1821 
               
               
                   
               
               
                 827327 
                 N/A 
                 N/A 
                 GGACTTTCTGTTGATG 
                 21 
                 19598 
                 19613 
                 1822 
               
               
                   
               
               
                 827328 
                 N/A 
                 N/A 
                 TGGACTTTCTGTTGAT 
                 29 
                 19599 
                 19614 
                 1823 
               
               
                   
               
               
                 827329 
                 N/A 
                 N/A 
                 CCATGGACTTTCTGTT 
                 32 
                 19602 
                 19617 
                 1824 
               
               
                   
                   
                   
                   
                   
                 19685 
                 19700 
                   
               
               
                   
               
               
                 827330 
                 N/A 
                 N/A 
                 TCCATGGACTTTCTGT 
                 28 
                 19603 
                 19618 
                 1825 
               
               
                   
               
               
                 827331 
                 N/A 
                 N/A 
                 GTCCATGGACTTTCTG 
                 41 
                 19604 
                 19619 
                 1826 
               
               
                   
               
               
                 827332 
                 N/A 
                 N/A 
                 GGACTTTCTGTTGAGG 
                 30 
                 19681 
                 19696 
                 1827 
               
               
                   
               
               
                 827335 
                 N/A 
                 N/A 
                 CGCCTAAGTGCCAAGA 
                 26 
                 19711 
                 19726 
                 1828 
               
               
                   
               
               
                 827336 
                 N/A 
                 N/A 
                 AGCAATGAGGCTCTGA 
                 30 
                 19738 
                 19753 
                 1829 
               
               
                   
               
               
                 827345 
                 N/A 
                 N/A 
                 ATAGTTACATGTGGTG 
                 25 
                 19863 
                 19878 
                 1830 
               
               
                   
               
               
                 827346 
                 N/A 
                 N/A 
                 AATAGTTACATGTGGT 
                 30 
                 19864 
                 19879 
                 1831 
               
               
                   
               
               
                 827347 
                 N/A 
                 N/A 
                 GAATAGTTACATGTGG 
                 13 
                 19865 
                 19880 
                 1832 
               
               
                   
               
               
                 827348 
                 N/A 
                 N/A 
                 TGGAATAGTTACATGT 
                 18 
                 19867 
                 19882 
                 1833 
               
               
                   
               
               
                 827349 
                 N/A 
                 N/A 
                 CTGGAATAGTTACATG 
                 25 
                 19868 
                 19883 
                 1834 
               
               
                   
               
               
                 827350 
                 N/A 
                 N/A 
                 ACTGGAATAGTTACAT 
                 47 
                 19869 
                 19884 
                 1835 
               
               
                   
               
               
                 827351 
                 N/A 
                 N/A 
                 TAACTGGAATAGTTAC 
                 93 
                 19871 
                 19886 
                 1836 
               
               
                   
               
               
                 827354 
                 N/A 
                 N/A 
                 GACTAACTGGAATAGT 
                 73 
                 19874 
                 19889 
                 1837 
               
               
                   
               
               
                 827355 
                 N/A 
                 N/A 
                 GGACTAACTGGAATAG 
                 33 
                 19875 
                 19890 
                 1838 
               
               
                   
               
               
                 827364 
                 N/A 
                 N/A 
                 GGAGGATACAGTTTGG 
                 32 
                 20588 
                 20603 
                 1839 
               
               
                   
               
               
                 827365 
                 N/A 
                 N/A 
                 ACACTGAACGATTTTA 
                 32 
                 20608 
                 20623 
                 1840 
               
               
                   
               
               
                 827366 
                 N/A 
                 N/A 
                 CTGGAGGCCGTGAGAG 
                 45 
                 20624 
                 20639 
                 1841 
               
               
                   
               
               
                 827367 
                 N/A 
                 N/A 
                 ACCAACTTGATGCTGG 
                 51 
                 20636 
                 20651 
                 1842 
               
               
                   
               
               
                 827368 
                 N/A 
                 N/A 
                 GGTGAGAAAGCCATGC 
                 17 
                 20660 
                 20675 
                 1843 
               
               
                   
               
               
                 827369 
                 N/A 
                 N/A 
                 GAAAAGGGTGTAGTTA 
                 35 
                 20690 
                 20705 
                 1844 
               
               
                   
               
               
                 827370 
                 N/A 
                 N/A 
                 AAACAGGTAGTGGTAA 
                 27 
                 20826 
                 20841 
                 1845 
               
               
                   
               
               
                 827371 
                 N/A 
                 N/A 
                 GTGAAATGTCCACCAC 
                 38 
                 20962 
                 20977 
                 1846 
               
               
                   
               
               
                 827374 
                 N/A 
                 N/A 
                 GCAAAAATGTGGGCCG 
                 50 
                 21420 
                 21435 
                 1847 
               
               
                   
               
               
                 827375 
                 N/A 
                 N/A 
                 TCCATGTACAGGATCC 
                 38 
                 21529 
                 21544 
                 1848 
               
               
                   
               
               
                 827383 
                 N/A 
                 N/A 
                 TAAGATGGCTAAAGTC 
                 13 
                 21733 
                 21748 
                 1849 
               
               
                   
               
               
                 827384 
                 N/A 
                 N/A 
                 GGATTCATTAAGATGG 
                 23 
                 21741 
                 21756 
                 1850 
               
               
                   
               
               
                 827385 
                 N/A 
                 N/A 
                 GGGATTCATTAAGATG 
                 31 
                 21742 
                 21757 
                 1851 
               
               
                   
               
               
                 827386 
                 N/A 
                 N/A 
                 AGGGATTCATTAAGAT 
                 14 
                 21743 
                 21758 
                 1852 
               
               
                   
               
               
                 827387 
                 N/A 
                 N/A 
                 CAGGGATTCATTAAGA 
                 30 
                 21744 
                 21759 
                 1853 
               
               
                   
               
               
                 827388 
                 N/A 
                 N/A 
                 ACAGGGATTCATTAAG 
                 38 
                 21745 
                 21760 
                 1854 
               
               
                   
               
               
                 827389 
                 N/A 
                 N/A 
                 TACAGGGATTCATTAA 
                 42 
                 21746 
                 21761 
                 1855 
               
               
                   
               
               
                 827390 
                 N/A 
                 N/A 
                 TTACAGGGATTCATTA 
                 55 
                 21747 
                 21762 
                 1856 
               
               
                   
               
               
                 827393 
                 N/A 
                 N/A 
                 TACGATTACAGGGATT 
                 11 
                 21752 
                 21767 
                 1857 
               
               
                   
               
               
                 827402 
                 N/A 
                 N/A 
                 GCTCACTAGTACGATT 
                 43 
                 21761 
                 21776 
                 1858 
               
               
                   
               
               
                 827403 
                 N/A 
                 N/A 
                 AGCTCACTAGTACGAT 
                 46 
                 21762 
                 21777 
                 1859 
               
               
                   
               
               
                 827404 
                 N/A 
                 N/A 
                 GCCTTAGTAAGAGCTG 
                 29 
                 21782 
                 21797 
                 1860 
               
               
                   
               
               
                 827405 
                 N/A 
                 N/A 
                 AGTTACTTACTTAATC 
                 28 
                 21896 
                 21911 
                 1861 
               
               
                   
               
               
                 827406 
                 N/A 
                 N/A 
                 GACCAAACAAGTTACT 
                 21 
                 21905 
                 21920 
                 1862 
               
               
                   
               
               
                 827407 
                 N/A 
                 N/A 
                 GGACCAAACAAGTTAC 
                 27 
                 21906 
                 21921 
                 1863 
               
               
                   
               
               
                 827408 
                 N/A 
                 N/A 
                 ATTAGATGTGGGACCA 
                 17 
                 21916 
                 21931 
                 1864 
               
               
                   
               
               
                 827411 
                 N/A 
                 N/A 
                 TATGAGAATCAGTATA 
                 56 
                 22311 
                 22326 
                 1865 
               
               
                   
               
               
                 827412 
                 N/A 
                 N/A 
                 CTATGAGAATCAGTAT 
                 44 
                 22312 
                 22327 
                 1866 
               
               
                   
               
               
                 827420 
                 N/A 
                 N/A 
                 GGAGAAACACGGATGG 
                 10 
                 22743 
                 22758 
                 1867 
               
               
                   
               
               
                 827421 
                 N/A 
                 N/A 
                 TTCCATCAGCGGTGGA 
                 52 
                 22756 
                 22771 
                 1868 
               
               
                   
               
               
                 827422 
                 N/A 
                 N/A 
                 GGCACAAGTTCCATCA 
                 23 
                 22764 
                 22779 
                 1869 
               
               
                   
               
               
                 827423 
                 N/A 
                 N/A 
                 AGGCACAAGTTCCATC 
                 34 
                 22765 
                 22780 
                 1870 
               
               
                   
               
               
                 827424 
                 N/A 
                 N/A 
                 CAGGCACAAGTTCCAT 
                 32 
                 22766 
                 22781 
                 1871 
               
               
                   
               
               
                 827425 
                 N/A 
                 N/A 
                 GCAGGCACAAGTTCCA 
                 19 
                 22767 
                 22782 
                 1872 
               
               
                   
               
               
                 827426 
                 N/A 
                 N/A 
                 AGCAGGCACAAGTTCC 
                 14 
                 22768 
                 22783 
                 1873 
               
               
                   
               
               
                 827427 
                 N/A 
                 N/A 
                 AAGCAGGCACAAGTTC 
                 44 
                 22769 
                 22784 
                 1874 
               
               
                   
               
               
                 827430 
                 N/A 
                 N/A 
                 GTGCTGCCCCCATGGA 
                 37 
                 22785 
                 22800 
                 1875 
               
               
                   
               
               
                 827431 
                 N/A 
                 N/A 
                 GGGACAAGTATAATGG 
                 58 
                 22804 
                 22819 
                 1876 
               
               
                   
               
               
                 827440 
                 N/A 
                 N/A 
                 AAGCAGGTCATTGTTT 
                 28 
                 23071 
                 23086 
                 1877 
               
               
                   
               
               
                 827441 
                 N/A 
                 N/A 
                 TGGTTGTACGGTCTCA 
                 19 
                 23086 
                 23101 
                 1878 
               
               
                   
               
               
                 827442 
                 N/A 
                 N/A 
                 GCAAAGACGGAAAGGG 
                 34 
                 23180 
                 23195 
                 1879 
               
               
                   
               
               
                 827443 
                 N/A 
                 N/A 
                 GCGACGGGAGCCAGGC 
                 36 
                 23194 
                 23209 
                 1880 
               
               
                   
               
               
                 827444 
                 N/A 
                 N/A 
                 CTTGGAGCTAGCGACG 
                 20 
                 23204 
                 23219 
                 1881 
               
               
                   
               
               
                 827445 
                 N/A 
                 N/A 
                 TGCTACCCTGCCATCT 
                 24 
                 23218 
                 23233 
                 1882 
               
               
                   
               
               
                 827446 
                 N/A 
                 N/A 
                 TGCCACACGGCACAGA 
                 64 
                 23248 
                 23263 
                 1883 
               
               
                   
               
               
                 827447 
                 N/A 
                 N/A 
                 GCAAATCACAGGTTCC 
                 19 
                 23302 
                 23317 
                 1884 
               
               
                   
               
               
                 827449 
                 N/A 
                 N/A 
                 CATCAGTATGTCTCAG 
                 18 
                 23412 
                 23427 
                 1885 
               
               
                   
               
               
                 827450 
                 N/A 
                 N/A 
                 GAGGAAGATCAGTACC 
                 39 
                 23451 
                 23466 
                 1886 
               
               
                   
               
               
                 827459 
                 N/A 
                 N/A 
                 CCCTAACTGCCCATGC 
                 20 
                 23711 
                 23726 
                 1887 
               
               
                   
               
               
                 827460 
                 N/A 
                 N/A 
                 CGGCATTGACTTCCGT 
                 48 
                 23775 
                 23790 
                 1888 
               
               
                   
               
               
                 827461 
                 N/A 
                 N/A 
                 TCACACATCTACCTTC 
                 34 
                 23828 
                 23843 
                 1889 
               
               
                   
               
               
                 827462 
                 N/A 
                 N/A 
                 TTTACTCACACTCCCT 
                 24 
                 23936 
                 23951 
                 1890 
               
               
                   
               
               
                 827463 
                 N/A 
                 N/A 
                 CCTACAGGACTTGTGC 
                 26 
                 24009 
                 24024 
                 1891 
               
               
                   
               
               
                 827464 
                 N/A 
                 N/A 
                 AGAGAGAGTAGGGTCA 
                 64 
                 24094 
                 24109 
                 1892 
               
               
                   
               
               
                 827465 
                 N/A 
                 N/A 
                 TGAGAGTAATTCCTTA 
                 50 
                 24117 
                 24132 
                 1893 
               
               
                   
               
               
                 827466 
                 N/A 
                 N/A 
                 CACCGTTGTTGATTCC 
                 39 
                 24212 
                 24227 
                 1894 
               
               
                   
               
               
                 827469 
                 N/A 
                 N/A 
                 GCTCAAGGTAAGTACA 
                 55 
                 24276 
                 24291 
                 1895 
               
               
                   
               
               
                 827470 
                 N/A 
                 N/A 
                 GCTCTAGGAGGTGAGC 
                 83 
                 24290 
                 24305 
                 1896 
               
               
                   
               
               
                 827479 
                 N/A 
                 N/A 
                 TCCTACTGGCCTCGCC 
                 49 
                 25036 
                 25051 
                 1897 
               
               
                   
               
               
                 827480 
                 N/A 
                 N/A 
                 TTCCAGGTTGTATCTC 
                 53 
                 25067 
                 25082 
                 1898 
               
               
                   
               
               
                 827481 
                 N/A 
                 N/A 
                 ACATACACCAAGAGAT 
                 16 
                 25127 
                 25142 
                 1899 
               
               
                   
               
               
                 827482 
                 N/A 
                 N/A 
                 CCTATGAACCCACATA 
                 107 
                 25138 
                 25153 
                 1900 
               
               
                   
               
               
                 827483 
                 N/A 
                 N/A 
                 GGCTGCCACGGAATCA 
                 50 
                 25265 
                 25280 
                 1901 
               
               
                   
               
               
                 827484 
                 N/A 
                 N/A 
                 ATACACAACCCCTCCA 
                 104 
                 25316 
                 25331 
                 1902 
               
               
                   
               
               
                 827485 
                 N/A 
                 N/A 
                 GTGAATACACACCTGG 
                 48 
                 25442 
                 25457 
                 1903 
               
               
                   
               
               
                 827486 
                 N/A 
                 N/A 
                 CCTCAGTGAGTACTGG 
                 52 
                 25602 
                 25617 
                 1904 
               
               
                   
               
               
                 827489 
                 N/A 
                 N/A 
                 GCCTGCAGGTTGTTTT 
                 56 
                 26100 
                 26115 
                 1905 
               
               
                   
               
               
                 827490 
                 N/A 
                 N/A 
                 TGAGGAACCGCTGGAG 
                 41 
                 26479 
                 26494 
                 1906 
               
               
                   
               
               
                 827499 
                 N/A 
                 N/A 
                 TCCAAACTTTACTGAT 
                 41 
                 26787 
                 26802 
                 1907 
               
               
                   
               
               
                 827500 
                 N/A 
                 N/A 
                 TAAGGAGGAGATTCCA 
                 38 
                 26809 
                 26824 
                 1908 
               
               
                   
               
               
                 827501 
                 N/A 
                 N/A 
                 GTCCTATACCAGGATA 
                 47 
                 26823 
                 26838 
                 1909 
               
               
                   
               
               
                 827502 
                 N/A 
                 N/A 
                 AGAGATTTGTCTAGTC 
                 12 
                 26836 
                 26851 
                 1910 
               
               
                   
               
               
                 827503 
                 N/A 
                 N/A 
                 ACTCAACTGTAGTCAA 
                 36 
                 26858 
                 26873 
                 1911 
               
               
                   
               
               
                 827504 
                 N/A 
                 N/A 
                 TGGCACACGACTTCCC 
                 28 
                 26883 
                 26898 
                 1912 
               
               
                   
               
               
                 827505 
                 N/A 
                 N/A 
                 TGCAAACCCTTGCAGC 
                 80 
                 26942 
                 26957 
                 1913 
               
               
                   
               
               
                 827506 
                 N/A 
                 N/A 
                 CACTACCATGTCCCCT 
                 60 
                 27075 
                 27090 
                 1914 
               
               
                   
               
               
                 827509 
                 N/A 
                 N/A 
                 TGCGGAGCCAGCCCAG 
                 43 
                 27202 
                 27217 
                 1915 
               
               
                   
               
               
                 827510 
                 N/A 
                 N/A 
                 CACGACTGGAAAGTCC 
                 42 
                 27232 
                 27247 
                 1916 
               
               
                   
               
               
                 827519 
                 N/A 
                 N/A 
                 TAATGGAACTGTAGAT 
                 31 
                 27734 
                 27749 
                 1917 
               
               
                   
               
               
                 827520 
                 N/A 
                 N/A 
                 TATATGATGATTGCAC 
                 35 
                 27883 
                 27898 
                 1918 
               
               
                   
               
               
                 827521 
                 N/A 
                 N/A 
                 TGCCTGGCTTGAGTGA 
                 47 
                 27944 
                 27959 
                 1919 
               
               
                   
               
               
                 827522 
                 N/A 
                 N/A 
                 GAGTACAAGGTTTATT 
                 22 
                 28237 
                 28252 
                 1920 
               
               
                   
               
               
                 827523 
                 N/A 
                 N/A 
                 TGAGTACAAGGTTTAT 
                 26 
                 28238 
                 28253 
                 1921 
               
               
                   
               
               
                 827524 
                 N/A 
                 N/A 
                 ATGAGTACAAGGTTTA 
                 7 
                 28239 
                 28254 
                 1922 
               
               
                   
               
               
                 827525 
                 N/A 
                 N/A 
                 GACTTGCTAATGAGTA 
                 36 
                 28248 
                 28263 
                 1923 
               
               
                   
               
               
                 827526 
                 N/A 
                 N/A 
                 GGACTTGCTAATGAGT 
                 58 
                 28249 
                 28264 
                 1924 
               
               
                   
               
               
                 827529 
                 N/A 
                 N/A 
                 TTGGGACTTGCTAATG 
                 53 
                 28252 
                 28267 
                 1925 
               
               
                   
               
               
                 827530 
                 N/A 
                 N/A 
                 GCTTGGGACTTGCTAA 
                 43 
                 28254 
                 28269 
                 1926 
               
               
                   
               
               
                 827538 
                 N/A 
                 N/A 
                 TTGCTACCATACGGAT 
                 23 
                 28747 
                 28762 
                 1927 
               
               
                   
               
               
                 827539 
                 N/A 
                 N/A 
                 ATTGCTACCATACGGA 
                 38 
                 28748 
                 28763 
                 1928 
               
               
                   
               
               
                 827540 
                 N/A 
                 N/A 
                 TATTGCTACCATACGG 
                 31 
                 28749 
                 28764 
                 1929 
               
               
                   
               
               
                 827541 
                 N/A 
                 N/A 
                 CTATTGCTACCATACG 
                 17 
                 28750 
                 28765 
                 1930 
               
               
                   
               
               
                 827542 
                 N/A 
                 N/A 
                 GCTATTGCTACCATAC 
                 31 
                 28751 
                 28766 
                 1931 
               
               
                   
               
               
                 827543 
                 N/A 
                 N/A 
                 CTGCTATTGCTACCAT 
                 25 
                 28753 
                 28768 
                 1932 
               
               
                   
               
               
                 827544 
                 N/A 
                 N/A 
                 AGGCACTGCTATTGCT 
                 42 
                 28758 
                 28773 
                 1933 
               
               
                   
               
               
                 827545 
                 N/A 
                 N/A 
                 CCATACAAGGGAGTGT 
                 66 
                 28799 
                 28814 
                 1934 
               
               
                   
               
               
                 827548 
                 N/A 
                 N/A 
                 TGCTGCTAGGGATGTA 
                 45 
                 29051 
                 29066 
                 1935 
               
               
                   
               
               
                 827549 
                 N/A 
                 N/A 
                 ACCCATTAAGATGTGT 
                 92 
                 29468 
                 29483 
                 1936 
               
               
                   
               
               
                 827558 
                 N/A 
                 N/A 
                 CCACACCCAAGAGGTC 
                 42 
                 30527 
                 30542 
                 1937 
               
               
                   
               
               
                 827559 
                 N/A 
                 N/A 
                 TCCTAGGGCACCTCAG 
                 100 
                 30554 
                 30569 
                 1938 
               
               
                   
               
               
                 827560 
                 N/A 
                 N/A 
                 TAGCAGTACCCTGTGG 
                 56 
                 30590 
                 30605 
                 1939 
               
               
                   
               
               
                 827561 
                 N/A 
                 N/A 
                 GGACACTAACCTGCAT 
                 47 
                 30669 
                 30684 
                 1940 
               
               
                   
               
               
                 827562 
                 N/A 
                 N/A 
                 ACCCAACCTGTACCCG 
                 57 
                 30692 
                 30707 
                 1941 
               
               
                   
               
               
                 827563 
                 N/A 
                 N/A 
                 GGCTCGGTAACCTGTA 
                 56 
                 30712 
                 30727 
                 1942 
               
               
                   
               
               
                 827564 
                 N/A 
                 N/A 
                 TCCCAAATGCTTGGCT 
                 58 
                 30724 
                 30739 
                 1943 
               
               
                   
               
               
                 827565 
                 N/A 
                 N/A 
                 GGCCACAGCATTACAT 
                 100 
                 30879 
                 30894 
                 1944 
               
               
                   
               
               
                 827568 
                 N/A 
                 N/A 
                 GGCTTACAGGGATAGG 
                 61 
                 30934 
                 30949 
                 1945 
               
               
                   
               
               
                 827569 
                 N/A 
                 N/A 
                 CCCATATGCTTCAGGC 
                 61 
                 30947 
                 30962 
                 1946 
               
               
                   
               
               
                 827578 
                 N/A 
                 N/A 
                 CCGACAGCCGCCCTGC 
                 43 
                 31323 
                 31338 
                 1947 
               
               
                   
               
               
                 827579 
                 N/A 
                 N/A 
                 GGCCGAGCTCCTTCTT 
                 71 
                 31435 
                 31450 
                 1948 
               
               
                   
               
               
                 827580 
                 N/A 
                 N/A 
                 ACCTTATGCCCCGGCC 
                 56 
                 31447 
                 31462 
                 1949 
               
               
                   
               
               
                 827581 
                 N/A 
                 N/A 
                 CCCCAGAGACCTTATG 
                 69 
                 31455 
                 31470 
                 1950 
               
               
                   
               
               
                 827582 
                 N/A 
                 N/A 
                 ACTGATAACTGGCCCA 
                 58 
                 31549 
                 31564 
                 1951 
               
               
                   
               
               
                 827583 
                 N/A 
                 N/A 
                 CACCAAGCTGTCTCCC 
                 50 
                 31655 
                 31670 
                 1952 
               
               
                   
               
               
                 827584 
                 N/A 
                 N/A 
                 GACGATGGGACAGAGG 
                 76 
                 31711 
                 31726 
                 1953 
               
               
                   
               
               
                 827585 
                 N/A 
                 N/A 
                 GAGGAGAGGTACATTG 
                 60 
                 31726 
                 31741 
                 1954 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 Table 5 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 SEQ ID: 1 
                 SEQ ID: 1 
                   
                 α-ENaC 
                 SEQ ID: 2 
                 SEQ ID 2: 
                   
               
               
                 Compound 
                 Start 
                 Stop 
                   
                 (% 
                 Start  
                 Stop 
                 SEQ ID 
               
               
                 Number 
                 Site 
                 Site 
                 Sequence 
                 control) 
                 Site 
                 Site 
                 NO 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 797469 
                 N/A 
                 N/A 
                 GGATGATGTGATACAT 
                 5 
                 18734 
                 18749 
                 400 
               
               
                   
               
               
                 797524 
                 N/A 
                 N/A 
                 ACCATACGGATGAACC 
                 23 
                 28742 
                 28757 
                 455 
               
               
                   
               
               
                 826071 
                 5 
                 20 
                 TTTAGACGCAGACAGG 
                 85 
                 4266 
                 4281 
                 466 
               
               
                   
               
               
                 826074 
                 25 
                 40 
                 GGCGGACTCTGGGCAG 
                 74 
                 4286 
                 4301 
                 611 
               
               
                   
               
               
                 826075 
                 28 
                 43 
                 GAAGGCGGACTCTGGG 
                 81 
                 4289 
                 4304 
                 612 
               
               
                   
               
               
                 826076 
                 29 
                 44 
                 AGAAGGCGGACTCTGG 
                 82 
                 4290 
                 4305 
                 613 
               
               
                   
               
               
                 826077 
                 31 
                 46 
                 TGAGAAGGCGGACTCT 
                 85 
                 4292 
                 4307 
                 614 
               
               
                   
               
               
                 826078 
                 32 
                 47 
                 CTGAGAAGGCGGACTC 
                 70 
                 4293 
                 4308 
                 615 
               
               
                   
               
               
                 826079 
                 33 
                 48 
                 CCTGAGAAGGCGGACT 
                 79 
                 4294 
                 4309 
                 616 
               
               
                   
               
               
                 826091 
                 53 
                 68 
                 GGTGAACTGGGAGTAC 
                 111 
                 4314 
                 4329 
                 468 
               
               
                   
               
               
                 826094 
                 73 
                 88 
                 AAGGAGGGCTCCCGAG 
                 66 
                 4334 
                 4349 
                 618 
               
               
                   
               
               
                 826095 
                 74 
                 89 
                 GAAGGAGGGCTCCCGA 
                 90 
                 4335 
                 4350 
                 619 
               
               
                   
               
               
                 826096 
                 81 
                 96 
                 TCCGAAGGAAGGAGGG 
                 81 
                 4342 
                 4357 
                 620 
               
               
                   
               
               
                 826097 
                 83 
                 98 
                 TTTCCGAAGGAAGGAG 
                 95 
                 4344 
                 4359 
                 621 
               
               
                   
               
               
                 826098 
                 85 
                 100 
                 GTTTTCCGAAGGAAGG 
                 69 
                 4346 
                 4361 
                 622 
               
               
                   
               
               
                 826099 
                 88 
                 103 
                 GGAGTTTTCCGAAGGA 
                 105 
                 4349 
                 4364 
                 623 
               
               
                   
               
               
                 826111 
                 163 
                 178 
                 GCGACAGGAATCTCAT 
                 90 
                 4424 
                 4439 
                 470 
               
               
                   
               
               
                 826114 
                 167 
                 182 
                 GGAAGCGACAGGAATC 
                 60 
                 4428 
                 4443 
                 626 
               
               
                   
               
               
                 826115 
                 169 
                 184 
                 ATGGAAGCGACAGGAA 
                 63 
                 4430 
                 4445 
                 627 
               
               
                   
               
               
                 826116 
                 170 
                 185 
                 GATGGAAGCGACAGGA 
                 53 
                 4431 
                 4446 
                 628 
               
               
                   
               
               
                 826117 
                 171 
                 186 
                 GGATGGAAGCGACAGG 
                 78 
                 4432 
                 4447 
                 629 
               
               
                   
               
               
                 826118 
                 172 
                 187 
                 GGGATGGAAGCGACAG 
                 38 
                 4433 
                 4448 
                 630 
               
               
                   
               
               
                 826119 
                 175 
                 190 
                 CCAGGGATGGAAGCGA 
                 56 
                 4436 
                 4451 
                 631 
               
               
                   
               
               
                 826131 
                 216 
                 231 
                 CAGGTGCAGCGGCCTG 
                 116 
                 4477 
                 4492 
                 472 
               
               
                   
               
               
                 826134 
                 226 
                 241 
                 GTTCCCCTGACAGGTG 
                 84 
                 N/A 
                 N/A 
                 634 
               
               
                   
               
               
                 826135 
                 228 
                 243 
                 TTGTTCCCCTGACAGG 
                 70 
                 N/A 
                 N/A 
                 635 
               
               
                   
               
               
                 826136 
                 229 
                 244 
                 CTTGTTCCCCTGACAG 
                 60 
                 N/A 
                 N/A 
                 636 
               
               
                   
               
               
                 826137 
                 230 
                 245 
                 GCTTGTTCCCCTGACA 
                 16 
                 N/A 
                 N/A 
                 637 
               
               
                   
               
               
                 826138 
                 232 
                 247 
                 CAGCTTGTTCCCCTGA 
                 52 
                 N/A 
                 N/A 
                 638 
               
               
                   
               
               
                 826139 
                 233 
                 248 
                 CCAGCTTGTTCCCCTG 
                 61 
                 N/A 
                 N/A 
                 639 
               
               
                   
               
               
                 826151 
                 283 
                 298 
                 CCCCTCCATGAGACCT 
                 26 
                 5211 
                 5226 
                 474 
               
               
                   
               
               
                 826154 
                 292 
                 307 
                 CAGCTTGTTCCCCTCC 
                 14 
                 5220 
                 5235 
                 642 
               
               
                   
               
               
                 826155 
                 310 
                 325 
                 GCTAGAGTCCTGCTCC 
                 43 
                 5238 
                 5253 
                 643 
               
               
                   
               
               
                 826156 
                 312 
                 327 
                 GGGCTAGAGTCCTGCT 
                 78 
                 5240 
                 5255 
                 644 
               
               
                   
               
               
                 826157 
                 315 
                 330 
                 GGAGGGCTAGAGTCCT 
                 94 
                 5243 
                 5258 
                 645 
               
               
                   
               
               
                 826158 
                 320 
                 335 
                 ACTGTGGAGGGCTAGA 
                 67 
                 5248 
                 5263 
                 646 
               
               
                   
               
               
                 826159 
                 321 
                 336 
                 GACTGTGGAGGGCTAG 
                 92 
                 5249 
                 5264 
                 647 
               
               
                   
               
               
                 826171 
                 355 
                 370 
                 CTCACGCTTGTTCCCC 
                 34 
                 5283 
                 5298 
                 476 
               
               
                   
               
               
                 826174 
                 360 
                 375 
                 TGCTCCTCACGCTTGT 
                 13 
                 5288 
                 5303 
                 650 
               
               
                   
               
               
                 826175 
                 362 
                 377 
                 CCTGCTCCTCACGCTT 
                 40 
                 5290 
                 5305 
                 651 
               
               
                   
               
               
                 826176 
                 363 
                 378 
                 CCCTGCTCCTCACGCT 
                 20 
                 5291 
                 5306 
                 652 
               
               
                   
               
               
                 826177 
                 386 
                 401 
                 GCGCCGCAGGTTCGGG 
                 49 
                 5314 
                 5329 
                 653 
               
               
                   
               
               
                 826178 
                 405 
                 420 
                 TCCGCCGTGGGCTGCT 
                 44 
                 5333 
                 5348 
                 654 
               
               
                   
               
               
                 826179 
                 407 
                 422 
                 CCTCCGCCGTGGGCTG 
                 36 
                 5335 
                 5350 
                 655 
               
               
                   
               
               
                 826191 
                 441 
                 456 
                 CGGTAGGAGCGGTGGA 
                 55 
                 5369 
                 5384 
                 478 
               
               
                   
               
               
                 826194 
                 446 
                 461 
                 GCTCTCGGTAGGAGCG 
                 60 
                 5374 
                 5389 
                 658 
               
               
                   
               
               
                 826195 
                 448 
                 463 
                 GAGCTCTCGGTAGGAG 
                 61 
                 5376 
                 5391 
                 959 
               
               
                   
               
               
                 826196 
                 451 
                 466 
                 GAAGAGCTCTCGGTAG 
                 48 
                 5379 
                 5394 
                 660 
               
               
                   
               
               
                 826197 
                 453 
                 468 
                 TCGAAGAGCTCTCGGT 
                 36 
                 5381 
                 5396 
                 661 
               
               
                   
               
               
                 826198 
                 456 
                 471 
                 AACTCGAAGAGCTCTC 
                 36 
                 5384 
                 5399 
                 662 
               
               
                   
               
               
                 826199 
                 457 
                 472 
                 GAACTCGAAGAGCTCT 
                 49 
                 5385 
                 5400 
                 663 
               
               
                   
               
               
                 826210 
                 566 
                 581 
                 TCATGCCAAAGGTGCA 
                 27 
                 5494 
                 5509 
                 480 
               
               
                   
               
               
                 826213 
                 575 
                 590 
                 GCCAGTACATCATGCC 
                 9 
                 5503 
                 5518 
                 666 
               
               
                   
               
               
                 826214 
                 577 
                 592 
                 TTGCCAGTACATCATG 
                 40 
                 5505 
                 5520 
                 667 
               
               
                   
               
               
                 826215 
                 580 
                 595 
                 GAATTGCCAGTACATC 
                 36 
                 5508 
                 5523 
                 668 
               
               
                   
               
               
                 826216 
                 581 
                 596 
                 CGAATTGCCAGTACAT 
                 26 
                 5509 
                 5524 
                 669 
               
               
                   
               
               
                 826217 
                 582 
                 597 
                 CCGAATTGCCAGTACA 
                 24 
                 5510 
                 5525 
                 670 
               
               
                   
               
               
                 826218 
                 585 
                 600 
                 AGGCCGAATTGCCAGT 
                 54 
                 5513 
                 5528 
                 671 
               
               
                   
               
               
                 826230 
                 607 
                 622 
                 GCTGAAGTACTCTCCG 
                 47 
                 5535 
                 5550 
                 482 
               
               
                   
               
               
                 826233 
                 626 
                 641 
                 TGTTGAGGCTGACGGG 
                 5 
                 5554 
                 5569 
                 674 
               
               
                   
               
               
                 826234 
                 628 
                 643 
                 GATGTTGAGGCTGACG 
                 31 
                 5556 
                 5571 
                 675 
               
               
                   
               
               
                 826235 
                 639 
                 654 
                 GAGTTGAGGTTGATGT 
                 34 
                 5567 
                 5582 
                 676 
               
               
                   
               
               
                 826236 
                 641 
                 656 
                 CCGAGTTGAGGTTGAT 
                 22 
                 5569 
                 5584 
                 677 
               
               
                   
               
               
                 826237 
                 643 
                 658 
                 GTCCGAGTTGAGGTTG 
                 30 
                 5571 
                 5586 
                 678 
               
               
                   
               
               
                 826238 
                 644 
                 659 
                 TGTCCGAGTTGAGGTT 
                 35 
                 5572 
                 5587 
                 679 
               
               
                   
               
               
                 826250 
                 703 
                 718 
                 AATTTCCGGGTACCTG 
                 29 
                 16288 
                 16303 
                 484 
               
               
                   
               
               
                 826253 
                 731 
                 746 
                 TGCGGTCCAGCTCCTC 
                 17 
                 16316 
                 16331 
                 682 
               
               
                   
               
               
                 826254 
                 734 
                 749 
                 TGATGCGGTCCAGCTC 
                 41 
                 16319 
                 16334 
                 683 
               
               
                   
               
               
                 826255 
                 737 
                 752 
                 CTGTGATGCGGTCCAG 
                 49 
                 16322 
                 16337 
                 684 
               
               
                   
               
               
                 826256 
                 739 
                 754 
                 CTCTGTGATGCGGTCC 
                 21 
                 16324 
                 16339 
                 685 
               
               
                   
               
               
                 826257 
                 740 
                 755 
                 GCTCTGTGATGCGGTC 
                 26 
                 16325 
                 16340 
                 686 
               
               
                   
               
               
                 826258 
                 759 
                 774 
                 TACAGGTCAAAGAGCG 
                 24 
                 16344 
                 16359 
                 687 
               
               
                   
               
               
                 826268 
                 792 
                 807 
                 CCGGCCACGAGAGTGG 
                 93 
                 16377 
                 16392 
                 486 
               
               
                   
               
               
                 826271 
                 798 
                 813 
                 CGGGAGCCGGCCACGA 
                 36 
                 16383 
                 16398 
                 690 
               
               
                   
               
               
                 826272 
                 800 
                 815 
                 TGCGGGAGCCGGCCAC 
                 39 
                 16385 
                 16400 
                 691 
               
               
                   
               
               
                 826273 
                 803 
                 818 
                 GGCTGCGGGAGCCGGC 
                 61 
                 16388 
                 16403 
                 692 
               
               
                   
               
               
                 826274 
                 804 
                 819 
                 CGGCTGCGGGAGCCGG 
                 71 
                 16389 
                 16404 
                 693 
               
               
                   
               
               
                 826275 
                 805 
                 820 
                 ACGGCTGCGGGAGCCG 
                 90 
                 16390 
                 16405 
                 694 
               
               
                   
               
               
                 826276 
                 807 
                 822 
                 CGACGGCTGCGGGAGC 
                 68 
                 16392 
                 16407 
                 695 
               
               
                   
               
               
                 826288 
                 830 
                 845 
                 GCGGCAGAGTCCCCCG 
                 62 
                 16415 
                 16430 
                 488 
               
               
                   
               
               
                 826291 
                 834 
                 849 
                 GGGTGCGGCAGAGTCC 
                 22 
                 16419 
                 16434 
                 698 
               
               
                   
               
               
                 826292 
                 858 
                 873 
                 GGCGGGACCCTCAGGC 
                 38 
                 16443 
                 16458 
                 699 
               
               
                   
               
               
                 826293 
                 877 
                 892 
                 ACGGGCCCCGTGAGGC 
                 46 
                 16462 
                 16477 
                 700 
               
               
                   
               
               
                 826294 
                 879 
                 894 
                 CGACGGGCCCCGTGAG 
                 54 
                 16464 
                 16479 
                 701 
               
               
                   
               
               
                 826295 
                 882 
                 897 
                 GCTCGACGGGCCCCGT 
                 37 
                 16467 
                 16482 
                 702 
               
               
                   
               
               
                 826296 
                 883 
                 898 
                 GGCTCGACGGGCCCCG 
                 46 
                 16468 
                 16483 
                 703 
               
               
                   
               
               
                 826308 
                 916 
                 931 
                 GTTGTTGTCCCGCAAG 
                 24 
                 16501 
                 16516 
                 490 
               
               
                   
               
               
                 826311 
                 952 
                 967 
                 GAAGCCGATCTTCCAG 
                 22 
                 16537 
                 16552 
                 706 
               
               
                   
               
               
                 826312 
                 953 
                 968 
                 GGAAGCCGATCTTCCA 
                 72 
                 16538 
                 16553 
                 707 
               
               
                   
               
               
                 826313 
                 954 
                 969 
                 TGGAAGCCGATCTTCC 
                 71 
                 16539 
                 16554 
                 708 
               
               
                   
               
               
                 826314 
                 956 
                 971 
                 GCTGGAAGCCGATCTT 
                 37 
                 16541 
                 16556 
                 709 
               
               
                   
               
               
                 826315 
                 958 
                 973 
                 CAGCTGGAAGCCGATC 
                 59 
                 16543 
                 16558 
                 710 
               
               
                   
               
               
                 826316 
                 968 
                 983 
                 TCTGGTTGCACAGCTG 
                 30 
                 N/A 
                 N/A 
                 711 
               
               
                   
               
               
                 826326 
                 980 
                 995 
                 AGTCCGATTTGTTCTG 
                 36 
                 17766 
                 17781 
                 492 
               
               
                   
               
               
                 826329 
                 983 
                 998 
                 AGCAGTCCGATTTGTT 
                 29 
                 17769 
                 17784 
                 714 
               
               
                   
               
               
                 826330 
                 985 
                 1000 
                 GAAGCAGTCCGATTTG 
                 51 
                 17771 
                 17786 
                 715 
               
               
                   
               
               
                 826331 
                 986 
                 1001 
                 AGAAGCAGTCCGATTT 
                 33 
                 17772 
                 17787 
                 716 
               
               
                   
               
               
                 826332 
                 987 
                 1002 
                 TAGAAGCAGTCCGATT 
                 61 
                 17773 
                 17788 
                 717 
               
               
                   
               
               
                 826333 
                 988 
                 1003 
                 GTAGAAGCAGTCCGAT 
                 44 
                 17774 
                 17789 
                 718 
               
               
                   
               
               
                 826334 
                 989 
                 1004 
                 GGTAGAAGCAGTCCGA 
                 18 
                 17775 
                 17790 
                 719 
               
               
                   
               
               
                 826346 
                 1018 
                 1033 
                 CACCGCATCCACCCCT 
                 42 
                 17804 
                 17819 
                 494 
               
               
                   
               
               
                 826349 
                 1022 
                 1037 
                 CCCTCACCGCATCCAC 
                 30 
                 17808 
                 17823 
                 722 
               
               
                   
               
               
                 826350 
                 1025 
                 1040 
                 ACTCCCTCACCGCATC 
                 39 
                 17811 
                 17826 
                 723 
               
               
                   
               
               
                 826351 
                 1026 
                 1041 
                 CACTCCCTCACCGCAT 
                 48 
                 17812 
                 17827 
                 724 
               
               
                   
               
               
                 826352 
                 1028 
                 1043 
                 ACCACTCCCTCACCGC 
                 32 
                 17814 
                 17829 
                 725 
               
               
                   
               
               
                 826353 
                 1032 
                 1047 
                 CGGTACCACTCCCTCA 
                 28 
                 17818 
                 17833 
                 726 
               
               
                   
               
               
                 826354 
                 1033 
                 1048 
                 GCGGTACCACTCCCTC 
                 46 
                 17819 
                 17834 
                 727 
               
               
                   
               
               
                 826366 
                 1115 
                 1130 
                 CGAAGATGAAGTTGCC 
                 67 
                 17901 
                 17916 
                 496 
               
               
                   
               
               
                 826369 
                 1123 
                 1138 
                 GCGGCAGGCGAAGATG 
                 62 
                 17909 
                 17924 
                 730 
               
               
                   
               
               
                 826370 
                 1126 
                 1141 
                 GAAGCGGCAGGCGAAG 
                 51 
                 17912 
                 17927 
                 731 
               
               
                   
               
               
                 826371 
                 1129 
                 1144 
                 GTTGAAGCGGCAGGCG 
                 43 
                 17915 
                 17930 
                 732 
               
               
                   
               
               
                 826372 
                 1130 
                 1145 
                 GGTTGAAGCGGCAGGC 
                 20 
                 17916 
                 17931 
                 733 
               
               
                   
               
               
                 826373 
                 1134 
                 1149 
                 ACCTGGTTGAAGCGGC 
                 28 
                 17920 
                 17935 
                 734 
               
               
                   
               
               
                 826374 
                 1136 
                 1151 
                 AGACCTGGTTGAAGCG 
                 31 
                 17922 
                 17937 
                 735 
               
               
                   
               
               
                 826386 
                 1164 
                 1179 
                 AAGTGAGAGTAATTCG 
                 42 
                 N/A 
                 N/A 
                 498 
               
               
                   
               
               
                 826389 
                 1232 
                 1247 
                 AAGACATCCAGAGGTT 
                 79 
                 24188 
                 24203 
                 738 
               
               
                   
               
               
                 826390 
                 1250 
                 1265 
                 TGTTGATTCCAGGCAT 
                 27 
                 24206 
                 24221 
                 739 
               
               
                   
               
               
                 826391 
                 1251 
                 1266 
                 TTGTTGATTCCAGGCA 
                 29 
                 24207 
                 24222 
                 740 
               
               
                   
               
               
                 826392 
                 1252 
                 1267 
                 GTTGTTGATTCCAGGC 
                 16 
                 24208 
                 24223 
                 741 
               
               
                   
               
               
                 826393 
                 1254 
                 1269 
                 CCGTTGTTGATTCCAG 
                 11 
                 24210 
                 24225 
                 742 
               
               
                   
               
               
                 826394 
                 1255 
                 1270 
                 ACCGTTGTTGATTCCA 
                 9 
                 24211 
                 24226 
                 743 
               
               
                   
               
               
                 826406 
                 1282 
                 1297 
                 CTGCTCTGCGCGCAGC 
                 56 
                 24579 
                 24594 
                 500 
               
               
                   
               
               
                 826409 
                 1285 
                 1300 
                 ATTCTGCTCTGCGCGC 
                 16 
                 24582 
                 24597 
                 746 
               
               
                   
               
               
                 826410 
                 1286 
                 1301 
                 CATTCTGCTCTGCGCG 
                 30 
                 24583 
                 24598 
                 747 
               
               
                   
               
               
                 826411 
                 1287 
                 1302 
                 TCATTCTGCTCTGCGC 
                 20 
                 24584 
                 24599 
                 748 
               
               
                   
               
               
                 826412 
                 1323 
                 1338 
                 CGGGCCCCAGTCACTG 
                 55 
                 24620 
                 24635 
                 749 
               
               
                   
               
               
                 826413 
                 1325 
                 1340 
                 CCCGGGCCCCAGTCAC 
                 51 
                 24622 
                 24637 
                 750 
               
               
                   
               
               
                 826414 
                 1327 
                 1342 
                 TACCCGGGCCCCAGTC 
                 38 
                 24624 
                 24639 
                 751 
               
               
                   
               
               
                 826426 
                 1356 
                 1371 
                 AAGGCAGGTTCATCCT 
                 122 
                 24653 
                 24668 
                 502 
               
               
                   
               
               
                 826429 
                 1366 
                 1381 
                 ATCATCCATAAAGGCA 
                 32 
                 24663 
                 24678 
                 754 
               
               
                   
               
               
                 826430 
                 1379 
                 1394 
                 AGTTAAAGCCACCATC 
                 37 
                 24676 
                 24691 
                 755 
               
               
                   
               
               
                 826431 
                 1383 
                 1398 
                 CGCAAGTTAAAGCCAC 
                 33 
                 24680 
                 24695 
                 756 
               
               
                   
               
               
                 826432 
                 1385 
                 1400 
                 GCCGCAAGTTAAAGCC 
                 43 
                 24682 
                 24697 
                 757 
               
               
                   
               
               
                 826433 
                 1387 
                 1402 
                 AGGCCGCAAGTTAAAG 
                 56 
                 24684 
                 24699 
                 758 
               
               
                   
               
               
                 826434 
                 1388 
                 1403 
                 CAGGCCGCAAGTTAAA 
                 42 
                 24685 
                 24700 
                 759 
               
               
                   
               
               
                 826446 
                 1416 
                 1431 
                 TTCCTCATGCTGATGG 
                 33 
                 24713 
                 24728 
                 504 
               
               
                   
               
               
                 826449 
                 1446 
                 1461 
                 TAATCGCCCCCAAGTC 
                 22 
                 25166 
                 25181 
                 762 
               
               
                   
               
               
                 826450 
                 1447 
                 1462 
                 ATAATCGCCCCCAAGT 
                 61 
                 25167 
                 25182 
                 763 
               
               
                   
               
               
                 826451 
                 1448 
                 1463 
                 CATAATCGCCCCCAAG 
                 17 
                 25168 
                 25183 
                 764 
               
               
                   
               
               
                 826452 
                 1450 
                 1465 
                 GCCATAATCGCCCCCA 
                 24 
                 25170 
                 25185 
                 765 
               
               
                   
               
               
                 826453 
                 1451 
                 1466 
                 CGCCATAATCGCCCCC 
                 25 
                 25171 
                 25186 
                 766 
               
               
                   
               
               
                 826454 
                 1453 
                 1468 
                 GTCGCCATAATCGCCC 
                 47 
                 25173 
                 25188 
                 767 
               
               
                   
               
               
                 826466 
                 1500 
                 1515 
                 GAAGGGTAAAGGTTCT 
                 60 
                 25220 
                 25235 
                 506 
               
               
                   
               
               
                 826469 
                 1528 
                 1543 
                 GTGAATACACACCTGC 
                 45 
                 N/A 
                 N/A 
                 770 
               
               
                   
               
               
                 826470 
                 1530 
                 1545 
                 GAGTGAATACACACCT 
                 58 
                 25444 
                 25459 
                 771 
               
               
                   
               
               
                 826471 
                 1531 
                 1546 
                 GGAGTGAATACACACC 
                 69 
                 25445 
                 25460 
                 772 
               
               
                   
               
               
                 826472 
                 1534 
                 1549 
                 GCAGGAGTGAATACAC 
                 73 
                 25448 
                 25463 
                 773 
               
               
                   
               
               
                 826473 
                 1553 
                 1568 
                 TGATCATGCTCTCCTG 
                 29 
                 25467 
                 25482 
                 774 
               
               
                   
               
               
                 826474 
                 1554 
                 1569 
                 TTGATCATGCTCTCCT 
                 19 
                 25468 
                 25483 
                 775 
               
               
                   
               
               
                 826485 
                 1579 
                 1594 
                 GAAGATGTAGGCACAG 
                 56 
                 25493 
                 25508 
                 507 
               
               
                   
               
               
                 826488 
                 1583 
                 1598 
                 GATAGAAGATGTAGGC 
                 26 
                 25497 
                 25512 
                 778 
               
               
                   
               
               
                 826489 
                 1584 
                 1599 
                 GGATAGAAGATGTAGG 
                 35 
                 25498 
                 25513 
                 779 
               
               
                   
               
               
                 826490 
                 1585 
                 1600 
                 CGGATAGAAGATGTAG 
                 47 
                 25499 
                 25514 
                 780 
               
               
                   
               
               
                 826491 
                 1587 
                 1602 
                 CGCGGATAGAAGATGT 
                 47 
                 25501 
                 25516 
                 781 
               
               
                   
               
               
                 826492 
                 1588 
                 1603 
                 CCGCGGATAGAAGATG 
                 71 
                 25502 
                 25517 
                 782 
               
               
                   
               
               
                 826493 
                 1589 
                 1604 
                 GCCGCGGATAGAAGAT 
                 62 
                 25503 
                 25518 
                 783 
               
               
                   
               
               
                 826505 
                 1661 
                 1676 
                 CAACCTGGAGCTTATA 
                 54 
                 30607 
                 30622 
                 509 
               
               
                   
               
               
                 826508 
                 1669 
                 1684 
                 GGAGAAGTCAACCTGG 
                 10 
                 30615 
                 30630 
                 786 
               
               
                   
               
               
                 826509 
                 1675 
                 1690 
                 GTCTGAGGAGAAGTCA 
                 32 
                 30621 
                 30636 
                 787 
               
               
                   
               
               
                 826510 
                 1696 
                 1711 
                 CTTGGTGAAACAGCCC 
                 53 
                 30642 
                 30657 
                 788 
               
               
                   
               
               
                 826511 
                 1702 
                 1717 
                 CCGGCACTTGGTGAAA 
                 92 
                 30648 
                 30663 
                 789 
               
               
                   
               
               
                 826512 
                 1708 
                 1723 
                 TGGCTTCCGGCACTTG 
                 25 
                 30654 
                 30669 
                 790 
               
               
                   
               
               
                 826513 
                 1709 
                 1724 
                 ATGGCTTCCGGCACTT 
                 34 
                 30655 
                 30670 
                 791 
               
               
                   
               
               
                 826525 
                 1736 
                 1751 
                 CAGAGAGCTGGTAGCT 
                 72 
                 30787 
                 30802 
                 511 
               
               
                   
               
               
                 826565 
                 1993 
                 2008 
                 GACGAGCTCAGCCATC 
                 39 
                 31822 
                 31837 
                 513 
               
               
                   
               
               
                 826568 
                 2001 
                 2016 
                 AGGTCAAAGACGAGCT 
                 29 
                 31830 
                 31845 
                 795 
               
               
                   
               
               
                 826569 
                 2002 
                 2017 
                 CAGGTCAAAGACGAGC 
                 28 
                 31831 
                 31846 
                 796 
               
               
                   
               
               
                 826570 
                 2003 
                 2018 
                 GCAGGTCAAAGACGAG 
                 42 
                 31832 
                 31847 
                 797 
               
               
                   
               
               
                 826571 
                 2009 
                 2024 
                 TGACCAGCAGGTCAAA 
                 106 
                 31838 
                 31853 
                 798 
               
               
                   
               
               
                 826572 
                 2011 
                 2026 
                 GATGACCAGCAGGTCA 
                 71 
                 31840 
                 31855 
                 799 
               
               
                   
               
               
                 826573 
                 2032 
                 2047 
                 TCGGAGCAGCATGAGG 
                 37 
                 31861 
                 31876 
                 800 
               
               
                   
               
               
                 826584 
                 2046 
                 2061 
                 CGGCTTCGGAACCTTC 
                 41 
                 31875 
                 31890 
                 514 
               
               
                   
               
               
                 826587 
                 2049 
                 2064 
                 TATCGGCTTCGGAACC 
                 43 
                 31878 
                 31893 
                 803 
               
               
                   
               
               
                 826588 
                 2050 
                 2065 
                 GTATCGGCTTCGGAAC 
                 30 
                 31879 
                 31894 
                 804 
               
               
                   
               
               
                 826589 
                 2051 
                 2066 
                 AGTATCGGCTTCGGAA 
                 27 
                 31880 
                 31895 
                 805 
               
               
                   
               
               
                 826590 
                 2053 
                 2068 
                 CCAGTATCGGCTTCGG 
                 23 
                 31882 
                 31897 
                 806 
               
               
                   
               
               
                 826591 
                 2054 
                 2069 
                 ACCAGTATCGGCTTCG 
                 12 
                 31883 
                 31898 
                 807 
               
               
                   
               
               
                 826592 
                 2055 
                 2070 
                 GACCAGTATCGGCTTC 
                 36 
                 31884 
                 31899 
                 808 
               
               
                   
               
               
                 826604 
                 2217 
                 2232 
                 CCCAGGGTGGCATAGG 
                 46 
                 32046 
                 32061 
                 516 
               
               
                   
               
               
                 826607 
                 2282 
                 2297 
                 AGGGCCCCCCCAGAGG 
                 97 
                 32111 
                 32126 
                 811 
               
               
                   
               
               
                 826608 
                 2284 
                 2299 
                 TCAGGGCCCCCCCAGA 
                 70 
                 32113 
                 32128 
                 812 
               
               
                   
               
               
                 826609 
                 2308 
                 2323 
                 GTGTGAGAAACCTCTC 
                 34 
                 32137 
                 32152 
                 813 
               
               
                   
               
               
                 826610 
                 2310 
                 2325 
                 TGGTGTGAGAAACCTC 
                 58 
                 32139 
                 32154 
                 814 
               
               
                   
               
               
                 826611 
                 2313 
                 2328 
                 CCTTGGTGTGAGAAAC 
                 46 
                 32142 
                 32157 
                 815 
               
               
                   
               
               
                 826612 
                 2314 
                 2329 
                 GCCTTGGTGTGAGAAA 
                 37 
                 32143 
                 32158 
                 816 
               
               
                   
               
               
                 826624 
                 2390 
                 2405 
                 TGGGCGGCTCTGAGAG 
                 52 
                 32219 
                 32234 
                 518 
               
               
                   
               
               
                 826627 
                 2399 
                 2414 
                 ACGGCAGTTTGGGCGG 
                 30 
                 32228 
                 32243 
                 819 
               
               
                   
               
               
                 826628 
                 2400 
                 2415 
                 AACGGCAGTTTGGGCG 
                 37 
                 32229 
                 32244 
                 820 
               
               
                   
               
               
                 826629 
                 2401 
                 2416 
                 CAACGGCAGTTTGGGC 
                 36 
                 32230 
                 32245 
                 821 
               
               
                   
               
               
                 826630 
                 2403 
                 2418 
                 ATCAACGGCAGTTTGG 
                 35 
                 32232 
                 32247 
                 822 
               
               
                   
               
               
                 826631 
                 2405 
                 2420 
                 ACATCAACGGCAGTTT 
                 16 
                 32234 
                 32249 
                 823 
               
               
                   
               
               
                 826632 
                 2407 
                 2422 
                 ACACATCAACGGCAGT 
                 15 
                 32236 
                 32251 
                 824 
               
               
                   
               
               
                 826644 
                 2476 
                 2491 
                 CTGGGCAGCTTCATCA 
                 42 
                 32305 
                 32320 
                 520 
               
               
                   
               
               
                 826647 
                 2491 
                 2506 
                 GGAGCCAAGGCACTTC 
                 13 
                 32320 
                 32335 
                 827 
               
               
                   
               
               
                 826648 
                 2492 
                 2507 
                 TGGAGCCAAGGCACTT 
                 29 
                 32321 
                 32336 
                 828 
               
               
                   
               
               
                 826649 
                 2502 
                 2517 
                 GGTACAGGGCTGGAGC 
                 52 
                 32331 
                 32346 
                 829 
               
               
                   
               
               
                 826650 
                 2520 
                 2535 
                 TCAGAGGCAGTACCAA 
                 30 
                 32349 
                 32364 
                 830 
               
               
                   
               
               
                 826651 
                 2523 
                 2538 
                 TGTTCAGAGGCAGTAC 
                 33 
                 32352 
                 32367 
                 831 
               
               
                   
               
               
                 826652 
                 2533 
                 2548 
                 GAAACCAGAGTGTTCA 
                 48 
                 32362 
                 32377 
                 832 
               
               
                   
               
               
                 826665 
                 2579 
                 2594 
                 CTTGGCTGATCCAAGG 
                 61 
                 32408 
                 32423 
                 835 
               
               
                   
               
               
                 826666 
                 2580 
                 2595 
                 GCTTGGCTGATCCAAG 
                 45 
                 32409 
                 32424 
                 836 
               
               
                   
               
               
                 826667 
                 2581 
                 2596 
                 CGCTTGGCTGATCCAA 
                 27 
                 32410 
                 32425 
                 837 
               
               
                   
               
               
                 826668 
                 2582 
                 2597 
                 TCGCTTGGCTGATCCA 
                 6 
                 32411 
                 32426 
                 838 
               
               
                   
               
               
                 826669 
                 2583 
                 2598 
                 TTCGCTTGGCTGATCC 
                 34 
                 32412 
                 32427 
                 839 
               
               
                   
               
               
                 826670 
                 2584 
                 2599 
                 TTTCGCTTGGCTGATC 
                 30 
                 32413 
                 32428 
                 840 
               
               
                   
               
               
                 826682 
                 2612 
                 2627 
                 TAGGAAAGTTCCTTGT 
                 83 
                 32441 
                 32456 
                 523 
               
               
                   
               
               
                 826685 
                 2623 
                 2638 
                 CAGCGGTTTCTTAGGA 
                 16 
                 32452 
                 32467 
                 843 
               
               
                   
               
               
                 826686 
                 2625 
                 2640 
                 ATCAGCGGTTTCTTAG 
                 42 
                 32454 
                 32469 
                 844 
               
               
                   
               
               
                 826687 
                 2627 
                 2642 
                 TTATCAGCGGTTTCTT 
                 22 
                 32456 
                 32471 
                 845 
               
               
                   
               
               
                 826688 
                 2629 
                 2644 
                 GGTTATCAGCGGTTTC 
                 9 
                 32458 
                 32473 
                 846 
               
               
                   
               
               
                 826689 
                 2632 
                 2647 
                 CCTGGTTATCAGCGGT 
                 17 
                 32461 
                 32476 
                 847 
               
               
                   
               
               
                 826690 
                 2634 
                 2649 
                 GTCCTGGTTATCAGCG 
                 25 
                 32463 
                 32478 
                 848 
               
               
                   
               
               
                 826702 
                 2681 
                 2696 
                 TGGGCAGGAAACCCGT 
                 58 
                 32510 
                 32525 
                 525 
               
               
                   
               
               
                 826705 
                 2692 
                 2707 
                 TAAGCCGTCGCTGGGC 
                 34 
                 32521 
                 32536 
                 851 
               
               
                   
               
               
                 826706 
                 2693 
                 2708 
                 TTAAGCCGTCGCTGGG 
                 47 
                 32522 
                 32537 
                 852 
               
               
                   
               
               
                 826707 
                 2696 
                 2711 
                 GGCTTAAGCCGTCGCT 
                 24 
                 32525 
                 32540 
                 853 
               
               
                   
               
               
                 826708 
                 2698 
                 2713 
                 CTGGCTTAAGCCGTCG 
                 42 
                 32527 
                 32542 
                 854 
               
               
                   
               
               
                 826709 
                 2700 
                 2715 
                 GGCTGGCTTAAGCCGT 
                 90 
                 32529 
                 32544 
                 855 
               
               
                   
               
               
                 826710 
                 2701 
                 2716 
                 GGGCTGGCTTAAGCCG 
                 83 
                 32530 
                 32545 
                 856 
               
               
                   
               
               
                 826722 
                 2835 
                 2850 
                 CCTAGCCCTCGGGAGT 
                 71 
                 32664 
                 32679 
                 527 
               
               
                   
               
               
                 826725 
                 2846 
                 2861 
                 TCTGCTCTAGCCCTAG 
                 52 
                 32675 
                 32690 
                 859 
               
               
                   
               
               
                 826726 
                 2847 
                 2862 
                 GTCTGCTCTAGCCCTA 
                 35 
                 32676 
                 32691 
                 860 
               
               
                   
               
               
                 826727 
                 2850 
                 2865 
                 CGGGTCTGCTCTAGCC 
                 61 
                 32679 
                 32694 
                 861 
               
               
                   
               
               
                 826728 
                 2852 
                 2867 
                 CCCGGGTCTGCTCTAG 
                 84 
                 32681 
                 32696 
                 862 
               
               
                   
               
               
                 826729 
                 2854 
                 2869 
                 TACCCGGGTCTGCTCT 
                 63 
                 32683 
                 32698 
                 863 
               
               
                   
               
               
                 826730 
                 2855 
                 2870 
                 TTACCCGGGTCTGCTC 
                 54 
                 32684 
                 32699 
                 864 
               
               
                   
               
               
                 826742 
                 2949 
                 2964 
                 TGTAGAGGTATGAAAG 
                 55 
                 32778 
                 32793 
                 529 
               
               
                   
               
               
                 826745 
                 2954 
                 2969 
                 AGACATGTAGAGGTAT 
                 17 
                 32783 
                 32798 
                 867 
               
               
                   
               
               
                 826746 
                 2955 
                 2970 
                 CAGACATGTAGAGGTA 
                 7 
                 32784 
                 32799 
                 868 
               
               
                   
               
               
                 826747 
                 2959 
                 2974 
                 CAAGCAGACATGTAGA 
                 31 
                 32788 
                 32803 
                 869 
               
               
                   
               
               
                 826748 
                 2960 
                 2975 
                 TCAAGCAGACATGTAG 
                 30 
                 32789 
                 32804 
                 870 
               
               
                   
               
               
                 826749 
                 2961 
                 2976 
                 CTCAAGCAGACATGTA 
                 24 
                 32790 
                 32805 
                 871 
               
               
                   
               
               
                 826750 
                 2963 
                 2978 
                 ATCTCAAGCAGACATG 
                 39 
                 32792 
                 32807 
                 872 
               
               
                   
               
               
                 826764 
                 3016 
                 3031 
                 ATGCATAGGAGTTCTC 
                 8 
                 32845 
                 32860 
                 875 
               
               
                   
               
               
                 826765 
                 3017 
                 3032 
                 GATGCATAGGAGTTCT 
                 30 
                 32846 
                 32861 
                 876 
               
               
                   
               
               
                 826766 
                 3019 
                 3034 
                 GGGATGCATAGGAGTT 
                 32 
                 32848 
                 32863 
                 877 
               
               
                   
               
               
                 826767 
                 3021 
                 3036 
                 AAGGGATGCATAGGAG 
                 31 
                 32850 
                 32865 
                 878 
               
               
                   
               
               
                 826768 
                 3022 
                 3037 
                 TAAGGGATGCATAGGA 
                 25 
                 32851 
                 32866 
                 879 
               
               
                   
               
               
                 826769 
                 3023 
                 3038 
                 CTAAGGGATGCATAGG 
                 35 
                 32852 
                 32867 
                 880 
               
               
                   
               
               
                 826781 
                 3117 
                 3132 
                 CTACGGGAGCCCAGGA 
                 32 
                 32946 
                 32961 
                 532 
               
               
                   
               
               
                 826784 
                 3121 
                 3136 
                 TGTGCTACGGGAGCCC 
                 8 
                 32950 
                 32965 
                 883 
               
               
                   
               
               
                 826785 
                 3122 
                 3137 
                 GTGTGCTACGGGAGCC 
                 20 
                 32951 
                 32966 
                 884 
               
               
                   
               
               
                 826786 
                 3123 
                 3138 
                 AGTGTGCTACGGGAGC 
                 15 
                 32952 
                 32967 
                 885 
               
               
                   
               
               
                 826787 
                 3124 
                 3139 
                 TAGTGTGCTACGGGAG 
                 21 
                 32953 
                 32968 
                 886 
               
               
                   
               
               
                 826788 
                 3125 
                 3140 
                 ATAGTGTGCTACGGGA 
                 29 
                 32954 
                 32969 
                 887 
               
               
                   
               
               
                 826789 
                 3126 
                 3141 
                 TATAGTGTGCTACGGG 
                 30 
                 32955 
                 32970 
                 888 
               
               
                   
               
               
                 826800 
                 3145 
                 3160 
                 GCAACACTCCAGCAGA 
                 17 
                 32974 
                 32989 
                 534 
               
               
                   
               
               
                 826803 
                 3153 
                 3168 
                 GTGCAACAGCAACACT 
                 36 
                 32982 
                 32997 
                 890 
               
               
                   
               
               
                 826804 
                 3154 
                 3169 
                 GGTGCAACAGCAACAC 
                 33 
                 32983 
                 32998 
                 891 
               
               
                   
               
               
                 826805 
                 3155 
                 3170 
                 TGGTGCAACAGCAACA 
                 30 
                 32984 
                 32999 
                 892 
               
               
                   
               
               
                 826806 
                 3156 
                 3171 
                 ATGGTGCAACAGCAAC 
                 11 
                 32985 
                 33000 
                 893 
               
               
                   
               
               
                 826807 
                 3157 
                 3172 
                 TATGGTGCAACAGCAA 
                 21 
                 32986 
                 33001 
                 894 
               
               
                   
               
               
                 826808 
                 3158 
                 3173 
                 GTATGGTGCAACAGCA 
                 20 
                 32987 
                 33002 
                 895 
               
               
                   
               
               
                 826818 
                 3213 
                 3228 
                 TGACCGCAAGGCACTT 
                 30 
                 33042 
                 33057 
                 536 
               
               
                   
               
               
                 826821 
                 3216 
                 3231 
                 CCCTGACCGCAAGGCA 
                 7 
                 33045 
                 33060 
                 897 
               
               
                   
               
               
                 826822 
                 3217 
                 3232 
                 TCCCTGACCGCAAGGC 
                 28 
                 33046 
                 33061 
                 898 
               
               
                   
               
               
                 826823 
                 3218 
                 3233 
                 GTCCCTGACCGCAAGG 
                 25 
                 33047 
                 33062 
                 899 
               
               
                   
               
               
                 826824 
                 3219 
                 3234 
                 AGTCCCTGACCGCAAG 
                 33 
                 33048 
                 33063 
                 900 
               
               
                   
               
               
                 826825 
                 3220 
                 3235 
                 CAGTCCCTGACCGCAA 
                 17 
                 33049 
                 33064 
                 901 
               
               
                   
               
               
                 826826 
                 3222 
                 3237 
                 TTCAGTCCCTGACCGC 
                 19 
                 33051 
                 33066 
                 902 
               
               
                   
               
               
                 826837 
                 3236 
                 3251 
                 ATAAACGGGCAAGATT 
                 57 
                 33065 
                 33080 
                 538 
               
               
                   
               
               
                 826840 
                 3239 
                 3254 
                 TACATAAACGGGCAAG 
                 40 
                 33068 
                 33083 
                 905 
               
               
                   
               
               
                 826841 
                 3242 
                 3257 
                 GCATACATAAACGGGC 
                 35 
                 33071 
                 33086 
                 906 
               
               
                   
               
               
                 826842 
                 3244 
                 3259 
                 GAGCATACATAAACGG 
                 27 
                 33073 
                 33088 
                 907 
               
               
                   
               
               
                 826843 
                 3249 
                 3264 
                 ACATGGAGCATACATA 
                 69 
                 33078 
                 33093 
                 908 
               
               
                   
               
               
                 826844 
                 3250 
                 3265 
                 GACATGGAGCATACAT 
                 42 
                 33079 
                 33094 
                 909 
               
               
                   
               
               
                 826845 
                 3251 
                 3266 
                 AGACATGGAGCATACA 
                 48 
                 33080 
                 33095 
                 910 
               
               
                   
               
               
                 826857 
                 N/A 
                 N/A 
                 TCCCCTTGGAAGGGAC 
                 109 
                 2713 
                 2728 
                 540 
               
               
                   
               
               
                 826860 
                 N/A 
                 N/A 
                 TGATACCTCCCCTTGG 
                 90 
                 2720 
                 2735 
                 913 
               
               
                   
               
               
                 826861 
                 N/A 
                 N/A 
                 ATGATACCTCCCCTTG 
                 84 
                 2721 
                 2736 
                 914 
               
               
                   
               
               
                 826862 
                 N/A 
                 N/A 
                 GCTCATGATACCTCCC 
                 68 
                 2725 
                 2740 
                 915 
               
               
                   
               
               
                 826863 
                 N/A 
                 N/A 
                 ACTGCTCATGATACCT 
                 89 
                 2728 
                 2743 
                 916 
               
               
                   
               
               
                 826864 
                 N/A 
                 N/A 
                 ATACTGCTCATGATAC 
                 70 
                 2730 
                 2745 
                 917 
               
               
                   
               
               
                 826865 
                 N/A 
                 N/A 
                 GATACTGCTCATGATA 
                 87 
                 2731 
                 2746 
                 918 
               
               
                   
               
               
                 826877 
                 N/A 
                 N/A 
                 GTCCTAGCACCTCCCT 
                 15 
                 4868 
                 4883 
                 542 
               
               
                   
               
               
                 826880 
                 N/A 
                 N/A 
                 CGAGTTTTGTCCTAGC 
                 7 
                 4876 
                 4891 
                 921 
               
               
                   
               
               
                 826881 
                 N/A 
                 N/A 
                 TCGAGTTTTGTCCTAG 
                 25 
                 4877 
                 4892 
                 922 
               
               
                   
               
               
                 826882 
                 N/A 
                 N/A 
                 CTTTCGAGTTTTGTCC 
                 36 
                 4880 
                 4895 
                 923 
               
               
                   
               
               
                 826883 
                 N/A 
                 N/A 
                 CACCTTTCGAGTTTTG 
                 26 
                 4883 
                 4898 
                 924 
               
               
                   
               
               
                 826884 
                 N/A 
                 N/A 
                 GCCACCTTTCGAGTTT 
                 30 
                 4885 
                 4900 
                 925 
               
               
                   
               
               
                 826885 
                 N/A 
                 N/A 
                 GGGCCACCTTTCGAGT 
                 27 
                 4887 
                 4902 
                 926 
               
               
                   
               
               
                 826897 
                 N/A 
                 N/A 
                 CTTCCCTAGAACGGCC 
                 37 
                 4923 
                 4938 
                 544 
               
               
                   
               
               
                 826900 
                 N/A 
                 N/A 
                 GCCGGAGCTGGGCTTC 
                 36 
                 4935 
                 4950 
                 929 
               
               
                   
               
               
                 826901 
                 N/A 
                 N/A 
                 TGCCGGAGCTGGGCTT 
                 80 
                 4936 
                 4951 
                 930 
               
               
                   
               
               
                 826902 
                 N/A 
                 N/A 
                 GTGCCGGAGCTGGGCT 
                 67 
                 4937 
                 4952 
                 931 
               
               
                   
               
               
                 826903 
                 N/A 
                 N/A 
                 AAGTGCCGGAGCTGGG 
                 30 
                 4939 
                 4954 
                 932 
               
               
                   
               
               
                 826904 
                 N/A 
                 N/A 
                 AAAAGTGCCGGAGCTG 
                 45 
                 4941 
                 4956 
                 933 
               
               
                   
               
               
                 826905 
                 N/A 
                 N/A 
                 CCAAAAGTGCCGGAGC 
                 28 
                 4943 
                 4958 
                 934 
               
               
                   
               
               
                 826917 
                 N/A 
                 N/A 
                 GAACCCGAGTGAGGCT 
                 39 
                 5059 
                 5074 
                 546 
               
               
                   
               
               
                 826920 
                 N/A 
                 N/A 
                 CCCCTGGAACCCGAGT 
                 25 
                 5065 
                 5080 
                 937 
               
               
                   
               
               
                 826921 
                 N/A 
                 N/A 
                 CACCCCTGGAACCCGA 
                 50 
                 5067 
                 5082 
                 938 
               
               
                   
               
               
                 826922 
                 N/A 
                 N/A 
                 CCCGGAGTGGATTGGG 
                 100 
                 5138 
                 5153 
                 939 
               
               
                   
               
               
                 826923 
                 N/A 
                 N/A 
                 GCCCCGGAGTGGATTG 
                 50 
                 5140 
                 5155 
                 940 
               
               
                   
               
               
                 826924 
                 N/A 
                 N/A 
                 GAGCCCCGGAGTGGAT 
                 46 
                 5142 
                 5157 
                 941 
               
               
                   
               
               
                 826925 
                 N/A 
                 N/A 
                 ATGAGCCCCGGAGTGG 
                 38 
                 5144 
                 5159 
                 942 
               
               
                   
               
               
                 826937 
                 N/A 
                 N/A 
                 AGCCGGGAAGGCCTCC 
                 124 
                 2486 
                 2501 
                 548 
               
               
                   
               
               
                 826940 
                 N/A 
                 N/A 
                 TGCTTACCTTGATACT 
                 113 
                 2741 
                 2756 
                 945 
               
               
                   
               
               
                 826941 
                 N/A 
                 N/A 
                 CCAAACCAGGTTCCCT 
                 125 
                 2757 
                 2772 
                 946 
               
               
                   
               
               
                 826942 
                 N/A 
                 N/A 
                 AGCCGGTGTCAACCAG 
                 103 
                 2777 
                 2792 
                 947 
               
               
                   
               
               
                 826943 
                 N/A 
                 N/A 
                 AAAGTGAAAGCCGGTG 
                 112 
                 2785 
                 2800 
                 948 
               
               
                   
               
               
                 826944 
                 N/A 
                 N/A 
                 TGCGACTTCTTAAAGT 
                 84 
                 2796 
                 2811 
                 949 
               
               
                   
               
               
                 826945 
                 N/A 
                 N/A 
                 GCTCAGGGTCCAACCT 
                 109 
                 2844 
                 2859 
                 950 
               
               
                   
               
               
                 826957 
                 N/A 
                 N/A 
                 GCCAAGTGGTGAGCAA 
                 42 
                 3338 
                 3353 
                 550 
               
               
                   
               
               
                 826960 
                 N/A 
                 N/A 
                 CGTTGATGGGCTATAT 
                 99 
                 3408 
                 3423 
                 953 
               
               
                   
               
               
                 826961 
                 N/A 
                 N/A 
                 CGCCTAGACAGGCCCT 
                 44 
                 3440 
                 3455 
                 954 
               
               
                   
               
               
                 826962 
                 N/A 
                 N/A 
                 ACGCAGGACACTGTGG 
                 80 
                 3555 
                 3570 
                 955 
               
               
                   
               
               
                 826963 
                 N/A 
                 N/A 
                 AGGCAGCGCGAGGGCC 
                 101 
                 3571 
                 3586 
                 956 
               
               
                   
               
               
                 826964 
                 N/A 
                 N/A 
                 GTGTAATCGCCCCTGC 
                 90 
                 3622 
                 3637 
                 957 
               
               
                   
               
               
                 826965 
                 N/A 
                 N/A 
                 GGCCCTAGGACATTCT 
                 79 
                 3674 
                 3689 
                 958 
               
               
                   
               
               
                 826977 
                 N/A 
                 N/A 
                 TGGGACTGGTTCCTTT 
                 94 
                 4536 
                 4551 
                 552 
               
               
                   
               
               
                 826980 
                 N/A 
                 N/A 
                 GGGACTAACCGACCTG 
                 98 
                 5631 
                 5646 
                 961 
               
               
                   
               
               
                 826981 
                 N/A 
                 N/A 
                 TTCCAGGCGCAGGCAC 
                 39 
                 5662 
                 5677 
                 862 
               
               
                   
               
               
                 826982 
                 N/A 
                 N/A 
                 CAGTAAGCTGGAGGCT 
                 92 
                 5785 
                 5800 
                 963 
               
               
                   
               
               
                 826983 
                 N/A 
                 N/A 
                 CGCCAGTCCAGTAAGC 
                 85 
                 5793 
                 5808 
                 964 
               
               
                   
               
               
                 826984 
                 N/A 
                 N/A 
                 GCTAGGATGGCTCCAC 
                 27 
                 5819 
                 5834 
                 965 
               
               
                   
               
               
                 826985 
                 N/A 
                 N/A 
                 CCACACTCTGGGTGAG 
                 36 
                 5843 
                 5858 
                 966 
               
               
                   
               
               
                 826997 
                 N/A 
                 N/A 
                 CCAGACCCAACATTGG 
                 84 
                 6361 
                 6376 
                 554 
               
               
                   
               
               
                 827000 
                 N/A 
                 N/A 
                 TCCCAAGGTGTGGCAT 
                 15 
                 6462 
                 6477 
                 969 
               
               
                   
               
               
                 827001 
                 N/A 
                 N/A 
                 TTGAAGCAGGTGTTCC 
                 57 
                 6475 
                 6490 
                 970 
               
               
                   
               
               
                 827002 
                 N/A 
                 N/A 
                 TGCCAGGTGCCTAGCC 
                 44 
                 6502 
                 6517 
                 971 
               
               
                   
               
               
                 827003 
                 N/A 
                 N/A 
                 CAATAAAGGGCTTATG 
                 64 
                 6538 
                 6553 
                 972 
               
               
                   
               
               
                 827004 
                 N/A 
                 N/A 
                 AACTACCTGGCCTTCA 
                 69 
                 6552 
                 6567 
                 973 
               
               
                   
               
               
                 827005 
                 N/A 
                 N/A 
                 GGCTTATATGCCTGTC 
                 68 
                 6605 
                 6620 
                 974 
               
               
                   
               
               
                 827017 
                 N/A 
                 N/A 
                 CTTTCTTAGTCCGTAA 
                 48 
                 6815 
                 6830 
                 556 
               
               
                   
               
               
                 827020 
                 N/A 
                 N/A 
                 AGGAAATGGTCCCTAC 
                 70 
                 6912 
                 6927 
                 977 
               
               
                   
               
               
                 827021 
                 N/A 
                 N/A 
                 GTGCACACGGCAGCTT 
                 52 
                 6932 
                 6947 
                 978 
               
               
                   
               
               
                 827022 
                 N/A 
                 N/A 
                 CCCAAGACACCTTCGC 
                 33 
                 6955 
                 6970 
                 979 
               
               
                   
               
               
                 827023 
                 N/A 
                 N/A 
                 TAGCACCGGGCTTGTA 
                 51 
                 6994 
                 7009 
                 980 
               
               
                   
               
               
                 827024 
                 N/A 
                 N/A 
                 AACAGGATGAGTCACA 
                 31 
                 7088 
                 7103 
                 981 
               
               
                   
               
               
                 827025 
                 N/A 
                 N/A 
                 AGTTTTGGGATTAGGC 
                 30 
                 7107 
                 7122 
                 982 
               
               
                   
               
               
                 827040 
                 N/A 
                 N/A 
                 GGGAATAATACTGCCC 
                 91 
                 7751 
                 7766 
                 985 
               
               
                   
               
               
                 827041 
                 N/A 
                 N/A 
                 AATGTATGTTCCCTTG 
                 27 
                 7816 
                 7831 
                 986 
               
               
                   
               
               
                 827042 
                 N/A 
                 N/A 
                 GTAAAAAGTCTGGCCC 
                 16 
                 8222 
                 8237 
                 987 
               
               
                   
               
               
                 827043 
                 N/A 
                 N/A 
                 TCCAAGGTGTGTTGTG 
                 22 
                 8283 
                 8298 
                 988 
               
               
                   
               
               
                 827044 
                 N/A 
                 N/A 
                 CATGAGACCTACTTCC 
                 26 
                 8296 
                 8311 
                 989 
               
               
                   
               
               
                 827045 
                 N/A 
                 N/A 
                 ATAAGAGTCATCATGA 
                 46 
                 8307 
                 8322 
                 990 
               
               
                   
               
               
                 827057 
                 N/A 
                 N/A 
                 TCGGTAGGAGTCATTC 
                 39 
                 8767 
                 8782 
                 559 
               
               
                   
               
               
                 827060 
                 N/A 
                 N/A 
                 CCTCAGCAGGTAGGCA 
                 60 
                 8836 
                 8851 
                 993 
               
               
                   
               
               
                 827061 
                 N/A 
                 N/A 
                 TCGGACTCAGCACTTC 
                 64 
                 8961 
                 8976 
                 994 
               
               
                   
               
               
                 827062 
                 N/A 
                 N/A 
                 CTGCAGTGGCCAACCC 
                 51 
                 8983 
                 8998 
                 995 
               
               
                   
               
               
                 827063 
                 N/A 
                 N/A 
                 CTGTAGGTATGACTGG 
                 18 
                 9047 
                 9062 
                 996 
               
               
                   
               
               
                 827064 
                 N/A 
                 N/A 
                 TTCCATGACTGTAGGT 
                 32 
                 9055 
                 9070 
                 997 
               
               
                   
               
               
                 827065 
                 N/A 
                 N/A 
                 GCCTAAACCGTTCCTG 
                 36 
                 9105 
                 9120 
                 998 
               
               
                   
               
               
                 827077 
                 N/A 
                 N/A 
                 TCTTACCCCGGTGGCC 
                 69 
                 9507 
                 9522 
                 561 
               
               
                   
               
               
                 827080 
                 N/A 
                 N/A 
                 GGCCTATCAACTAGGC 
                 103 
                 9783 
                 9798 
                 1001 
               
               
                   
               
               
                 827081 
                 N/A 
                 N/A 
                 CACAATTCCATCGGGC 
                 17 
                 9837 
                 9852 
                 1002 
               
               
                   
               
               
                 827082 
                 N/A 
                 N/A 
                 CCCTACATTGGAGGGT 
                 91 
                 9866 
                 9881 
                 1003 
               
               
                   
               
               
                 827083 
                 N/A 
                 N/A 
                 AGGGATAAAGAATGCC 
                 38 
                 9978 
                 9993 
                 1004 
               
               
                   
               
               
                 827084 
                 N/A 
                 N/A 
                 GACCAGCGGCTGGAGG 
                 54 
                 9996 
                 10011 
                 1005 
               
               
                   
               
               
                 827085 
                 N/A 
                 N/A 
                 AGACATCCGATCTTGT 
                 41 
                 10020 
                 10035 
                 1006 
               
               
                   
               
               
                 827097 
                 N/A 
                 N/A 
                 CTGCCACCCTACGCGC 
                 54 
                 10635 
                 10650 
                 563 
               
               
                   
               
               
                 827100 
                 N/A 
                 N/A 
                 TACGCACCTCCCTCCT 
                 35 
                 10649 
                 10664 
                 1009 
               
               
                   
                   
                   
                   
                   
                 10672 
                 10687 
                   
               
               
                   
               
               
                 827101 
                 N/A 
                 N/A 
                 CTACGCACCTCCCTCC 
                 64 
                 10650 
                 10665 
                 1010 
               
               
                   
                   
                   
                   
                   
                 10673 
                 10688 
                   
               
               
                   
               
               
                 827102 
                 N/A 
                 N/A 
                 CCCTACGCACCTCCCT 
                 60 
                 10652 
                 10667 
                 1011 
               
               
                   
                   
                   
                   
                   
                 10675 
                 10690 
                   
               
               
                   
               
               
                 827103 
                 N/A 
                 N/A 
                 ACCCTACGCACCTCCC 
                 34 
                 10653 
                 10668 
                 1012 
               
               
                   
                   
                   
                   
                   
                 10676 
                 10691 
                   
               
               
                   
               
               
                 827104 
                 N/A 
                 N/A 
                 CCACCCTACGCACCTC 
                 36 
                 10655 
                 10670 
                 1013 
               
               
                   
                   
                   
                   
                   
                 10678 
                 10693 
                   
               
               
                   
               
               
                 827105 
                 N/A 
                 N/A 
                 GCCACCCTACGCACCT 
                 49 
                 10656 
                 10671 
                 1014 
               
               
                   
                   
                   
                   
                   
                 10679 
                 10694 
                   
               
               
                   
               
               
                 827117 
                 N/A 
                 N/A 
                 GGGCATAACACTAGAT 
                 53 
                 11100 
                 11115 
                 565 
               
               
                   
               
               
                 827120 
                 N/A 
                 N/A 
                 CCACATGGTGCCCCAG 
                 40 
                 11248 
                 11263 
                 1017 
               
               
                   
               
               
                 827121 
                 N/A 
                 N/A 
                 TTTTAGGAGGGCCACA 
                 60 
                 11259 
                 11274 
                 1018 
               
               
                   
               
               
                 827122 
                 N/A 
                 N/A 
                 GCCCTCTGGTCCGTCC 
                 40 
                 11291 
                 11306 
                 1019 
               
               
                   
               
               
                 827123 
                 N/A 
                 N/A 
                 GGTCAGACAGCACTCC 
                 36 
                 11319 
                 11334 
                 1020 
               
               
                   
               
               
                 827124 
                 N/A 
                 N/A 
                 AGCTAGCAAATGGGTC 
                 56 
                 11331 
                 11346 
                 1021 
               
               
                   
               
               
                 827125 
                 N/A 
                 N/A 
                 TTCCAGTTGGCACAGC 
                 26 
                 11344 
                 11359 
                 1022 
               
               
                   
               
               
                 827137 
                 N/A 
                 N/A 
                 GGTTACACCCCCGGCG 
                 44 
                 11650 
                 11665 
                 567 
               
               
                   
               
               
                 827140 
                 N/A 
                 N/A 
                 CCCACAGAAAACGGAA 
                 74 
                 11690 
                 11705 
                 1025 
               
               
                   
               
               
                 827141 
                 N/A 
                 N/A 
                 GGCTGCTGCATGATTC 
                 33 
                 11738 
                 11753 
                 1026 
               
               
                   
               
               
                 827142 
                 N/A 
                 N/A 
                 ACCAGAATAGATTCAC 
                 63 
                 11766 
                 11781 
                 1027 
               
               
                   
               
               
                 827143 
                 N/A 
                 N/A 
                 TCGAATCGAGTGCCCC 
                 40 
                 11791 
                 11806 
                 1028 
               
               
                   
               
               
                 827144 
                 N/A 
                 N/A 
                 AACAATGAACCTCGAA 
                 51 
                 11802 
                 11817 
                 1029 
               
               
                   
               
               
                 827145 
                 N/A 
                 N/A 
                 TGGTATTAGAATGTAC 
                 19 
                 11881 
                 11896 
                 1030 
               
               
                   
               
               
                 827157 
                 N/A 
                 N/A 
                 TTGAAAGAGCCCCCAC 
                 65 
                 12166 
                 12181 
                 569 
               
               
                   
               
               
                 827160 
                 N/A 
                 N/A 
                 GTGCAGGGTCTTACTT 
                 19 
                 12230 
                 12245 
                 1033 
               
               
                   
               
               
                 827161 
                 N/A 
                 N/A 
                 AAATACCAGTGCAGGG 
                 41 
                 12238 
                 12253 
                 1034 
               
               
                   
               
               
                 827162 
                 N/A 
                 N/A 
                 GTACATCAATTATGCC 
                 36 
                 12268 
                 12283 
                 1035 
               
               
                   
               
               
                 827163 
                 N/A 
                 N/A 
                 GGGCACTCAAGATTTG 
                 62 
                 12295 
                 12310 
                 1036 
               
               
                   
               
               
                 827164 
                 N/A 
                 N/A 
                 CAAACCTGAGTGGGCA 
                 36 
                 12306 
                 12321 
                 1037 
               
               
                   
               
               
                 827165 
                 N/A 
                 N/A 
                 CTCGACTGTCAAACCT 
                 30 
                 12315 
                 12330 
                 1038 
               
               
                   
               
               
                 827177 
                 N/A 
                 N/A 
                 GCAATCATAGCTAGCA 
                 57 
                 12729 
                 12744 
                 571 
               
               
                   
               
               
                 827180 
                 N/A 
                 N/A 
                 GTTGAAGGTGTGTGTT 
                 35 
                 13095 
                 13110 
                 1041 
               
               
                   
               
               
                 827181 
                 N/A 
                 N/A 
                 AGCAACTCAAAGGTGT 
                 22 
                 13111 
                 13126 
                 1042 
               
               
                   
               
               
                 827182 
                 N/A 
                 N/A 
                 AGATTTGTACATGAGG 
                 30 
                 13481 
                 13496 
                 1043 
               
               
                   
               
               
                 827183 
                 N/A 
                 N/A 
                 ACCCGAAACACATTAG 
                 61 
                 13504 
                 13519 
                 1044 
               
               
                   
               
               
                 827184 
                 N/A 
                 N/A 
                 GTTTAGGCCGCACCCG 
                 36 
                 13515 
                 13530 
                 1045 
               
               
                   
               
               
                 827185 
                 N/A 
                 N/A 
                 ATTTACGGTGTTTAGG 
                 42 
                 13524 
                 13539 
                 1046 
               
               
                   
               
               
                 827197 
                 N/A 
                 N/A 
                 GTAGGCACTTTATGAA 
                 68 
                 14142 
                 14157 
                 573 
               
               
                   
               
               
                 827200 
                 N/A 
                 N/A 
                 AACCGTATGTAGTAGG 
                 18 
                 14153 
                 14168 
                 1049 
               
               
                   
               
               
                 827201 
                 N/A 
                 N/A 
                 CAACCGTATGTAGTAG 
                 25 
                 14154 
                 14169 
                 1050 
               
               
                   
               
               
                 827202 
                 N/A 
                 N/A 
                 ACAACCGTATGTAGTA 
                 40 
                 14155 
                 14170 
                 1051 
               
               
                   
               
               
                 827203 
                 N/A 
                 N/A 
                 AACAACCGTATGTAGT 
                 36 
                 14156 
                 14171 
                 1052 
               
               
                   
               
               
                 827204 
                 N/A 
                 N/A 
                 GAACAACCGTATGTAG 
                 39 
                 14157 
                 14172 
                 1053 
               
               
                   
               
               
                 827216 
                 N/A 
                 N/A 
                 GGAGAGACAATAGATC 
                 50 
                 14488 
                 14503 
                 575 
               
               
                   
               
               
                 827219 
                 N/A 
                 N/A 
                 TGACATACTGCTTCTA 
                 33 
                 14642 
                 14657 
                 1056 
               
               
                   
               
               
                 827220 
                 N/A 
                 N/A 
                 CCCCAGCAGGTATTTT 
                 98 
                 14667 
                 14682 
                 1057 
               
               
                   
               
               
                 827221 
                 N/A 
                 N/A 
                 CCCAAGCAATCACCAG 
                 61 
                 14737 
                 14752 
                 1058 
               
               
                   
               
               
                 827222 
                 N/A 
                 N/A 
                 GACCAAAAGTGTGCCA 
                 54 
                 14831 
                 14846 
                 1059 
               
               
                   
               
               
                 827223 
                 N/A 
                 N/A 
                 GACACAATCGCCGCTC 
                 64 
                 14905 
                 14920 
                 1060 
               
               
                   
               
               
                 827224 
                 N/A 
                 N/A 
                 GAATAAGTGGAGATAT 
                 84 
                 15017 
                 15032 
                 1061 
               
               
                   
               
               
                 827236 
                 N/A 
                 N/A 
                 CCGCAGGCGAGTGTCG 
                 61 
                 15878 
                 15893 
                 577 
               
               
                   
               
               
                 827239 
                 N/A 
                 N/A 
                 CCGGACCTAGAAGGGA 
                 89 
                 15987 
                 16002 
                 1064 
               
               
                   
               
               
                 827240 
                 N/A 
                 N/A 
                 GGCCACGGCGAGCCCA 
                 82 
                 16080 
                 16095 
                 1065 
               
               
                   
               
               
                 827241 
                 N/A 
                 N/A 
                 GTAAACAGGTGTGTCC 
                 48 
                 16110 
                 16125 
                 1066 
               
               
                   
               
               
                 827242 
                 N/A 
                 N/A 
                 CTGGAGCGAGTGTCTG 
                 93 
                 16238 
                 16253 
                 1067 
               
               
                   
               
               
                 827243 
                 N/A 
                 N/A 
                 GCGGAGCCCATGGGTG 
                 52 
                 16616 
                 16631 
                 1068 
               
               
                   
               
               
                 827244 
                 N/A 
                 N/A 
                 TGTCACTGGGCTGCGC 
                 41 
                 16650 
                 16665 
                 1069 
               
               
                   
               
               
                 827256 
                 N/A 
                 N/A 
                 CAAGAGATTTGTCCCA 
                 67 
                 17420 
                 17435 
                 579 
               
               
                   
               
               
                 827259 
                 N/A 
                 N/A 
                 ATTTATACCTCCCCTG 
                 60 
                 17495 
                 17510 
                 1072 
               
               
                   
               
               
                 827260 
                 N/A 
                 N/A 
                 CACACACGGTTTTGGT 
                 26 
                 17513 
                 17528 
                 1073 
               
               
                   
               
               
                 827261 
                 N/A 
                 N/A 
                 GACCAGTAGCTGCACA 
                 34 
                 17527 
                 17542 
                 1074 
               
               
                   
               
               
                 827262 
                 N/A 
                 N/A 
                 ATTAAGGGAGTTGCAG 
                 61 
                 17555 
                 17570 
                 1075 
               
               
                   
               
               
                 827263 
                 N/A 
                 N/A 
                 CCCTAGGAGCATGGAC 
                 47 
                 17585 
                 17600 
                 1076 
               
               
                   
               
               
                 827264 
                 N/A 
                 N/A 
                 GCAGAAGTCCCTAGGA 
                 61 
                 17593 
                 17608 
                 1077 
               
               
                   
               
               
                 827276 
                 N/A 
                 N/A 
                 CCTCAGATCCAGCAGT 
                 49 
                 18147 
                 18162 
                 581 
               
               
                   
               
               
                 827279 
                 N/A 
                 N/A 
                 AGATCCATGCTTCCAG 
                 11 
                 18216 
                 18231 
                 1080 
               
               
                   
               
               
                 827280 
                 N/A 
                 N/A 
                 AAGATCCATGCTTCCA 
                 17 
                 18217 
                 18232 
                 1081 
               
               
                   
               
               
                 827281 
                 N/A 
                 N/A 
                 CCAAGATCCATGCTTC 
                 20 
                 18219 
                 18234 
                 1082 
               
               
                   
               
               
                 827282 
                 N/A 
                 N/A 
                 ACCAAGATCCATGCTT 
                 35 
                 18220 
                 18235 
                 1083 
               
               
                   
               
               
                 827283 
                 N/A 
                 N/A 
                 GACCAAGATCCATGCT 
                 13 
                 18221 
                 18236 
                 1084 
               
               
                   
               
               
                 827295 
                 N/A 
                 N/A 
                 GATACATCCAGAGTCA 
                 36 
                 18725 
                 18740 
                 583 
               
               
                   
               
               
                 827298 
                 N/A 
                 N/A 
                 AGGATGATGTGATACA 
                 24 
                 18735 
                 18750 
                 1087 
               
               
                   
               
               
                 827299 
                 N/A 
                 N/A 
                 AAGGATGATGTGATAC 
                 36 
                 18736 
                 18751 
                 1088 
               
               
                   
               
               
                 827300 
                 N/A 
                 N/A 
                 ATCTAAGAAATAGGCT 
                 29 
                 18755 
                 18770 
                 1089 
               
               
                   
               
               
                 827301 
                 N/A 
                 N/A 
                 CACATAGCCCAGATAG 
                 21 
                 18834 
                 18849 
                 1090 
               
               
                   
               
               
                 827302 
                 N/A 
                 N/A 
                 TGCCAAAGGAGCATGG 
                 52 
                 18901 
                 18916 
                 1091 
               
               
                   
               
               
                 827314 
                 N/A 
                 N/A 
                 GGAGATGGCTCCGGAA 
                 63 
                 19278 
                 19293 
                 585 
               
               
                   
               
               
                 827317 
                 N/A 
                 N/A 
                 GGTAAGAAGTGACACC 
                 62 
                 19364 
                 19379 
                 1094 
               
               
                   
               
               
                 827318 
                 N/A 
                 N/A 
                 GTGTACTGGGCAGAGT 
                 16 
                 19390 
                 19405 
                 1095 
               
               
                   
               
               
                 827319 
                 N/A 
                 N/A 
                 TGCTACCATCTTACTT 
                 26 
                 19463 
                 19478 
                 1096 
               
               
                   
               
               
                 827320 
                 N/A 
                 N/A 
                 GGCTTAGGTGTTGCTA 
                 28 
                 19474 
                 19489 
                 1097 
               
               
                   
               
               
                 827321 
                 N/A 
                 N/A 
                 GCGGACTCAGGCTTAG 
                 50 
                 19483 
                 19498 
                 1098 
               
               
                   
               
               
                 827322 
                 N/A 
                 N/A 
                 TGACAGGTGTGGGCGG 
                 46 
                 19495 
                 19510 
                 1099 
               
               
                   
               
               
                 827334 
                 N/A 
                 N/A 
                 AACCATGGACTTTCTG 
                 46 
                 19687 
                 19702 
                 587 
               
               
                   
               
               
                 827337 
                 N/A 
                 N/A 
                 CCCAGGCGAGCAATGA 
                 49 
                 19746 
                 19761 
                 1102 
               
               
                   
               
               
                 827338 
                 N/A 
                 N/A 
                 GGTATAACAACCCAGG 
                 34 
                 19756 
                 19771 
                 1103 
               
               
                   
               
               
                 827339 
                 N/A 
                 N/A 
                 CAGTAGGGTGGAGTGG 
                 41 
                 19774 
                 19789 
                 1104 
               
               
                   
               
               
                 827340 
                 N/A 
                 N/A 
                 GTACAAAGGTTCCTGT 
                 48 
                 19829 
                 19844 
                 1105 
               
               
                   
               
               
                 827341 
                 N/A 
                 N/A 
                 CGTGAAGTAAGGTTGA 
                 25 
                 19846 
                 19861 
                 1106 
               
               
                   
               
               
                 827342 
                 N/A 
                 N/A 
                 GTTACATGTGGTGACG 
                 43 
                 19860 
                 19875 
                 1107 
               
               
                   
               
               
                 827353 
                 N/A 
                 N/A 
                 ACTAACTGGAATAGTT 
                 99 
                 19873 
                 19888 
                 589 
               
               
                   
               
               
                 827356 
                 N/A 
                 N/A 
                 AGGACTAACTGGAATA 
                 15 
                 19876 
                 19891 
                 1110 
               
               
                   
               
               
                 827357 
                 N/A 
                 N/A 
                 CAGGACTAACTGGAAT 
                 31 
                 19877 
                 19892 
                 1111 
               
               
                   
               
               
                 827358 
                 N/A 
                 N/A 
                 GCCCGGTGAGATATTC 
                 55 
                 19923 
                 19938 
                 1112 
               
               
                   
               
               
                 827359 
                 N/A 
                 N/A 
                 CCCGATAGCTGGTTGT 
                 11 
                 20415 
                 20430 
                 1113 
               
               
                   
               
               
                 827360 
                 N/A 
                 N/A 
                 TTAATTAGTTCACCCG 
                 4 
                 20427 
                 20442 
                 1114 
               
               
                   
               
               
                 827361 
                 N/A 
                 N/A 
                 AGTGAATCCTCACACT 
                 88 
                 20444 
                 20459 
                 1115 
               
               
                   
               
               
                 827373 
                 N/A 
                 N/A 
                 AAAAAGGTGGTGTATC 
                 80 
                 21111 
                 21126 
                 591 
               
               
                   
               
               
                 827376 
                 N/A 
                 N/A 
                 TCATAGGTAAACACCC 
                 14 
                 21565 
                 21580 
                 1118 
               
               
                   
               
               
                 827377 
                 N/A 
                 N/A 
                 GAAAAGTCTGGTAGCT 
                 23 
                 21628 
                 21643 
                 1119 
               
               
                   
               
               
                 827378 
                 N/A 
                 N/A 
                 TGGTGTGACCATTTGG 
                 9 
                 21643 
                 21658 
                 1120 
               
               
                   
               
               
                 827379 
                 N/A 
                 N/A 
                 ATGGTGTGACCATTTG 
                 15 
                 21644 
                 21659 
                 1121 
               
               
                   
               
               
                 827380 
                 N/A 
                 N/A 
                 AAATGGTGTGACCATT 
                 46 
                 21646 
                 21661 
                 1122 
               
               
                   
               
               
                 827381 
                 N/A 
                 N/A 
                 TAAATGGTGTGACCAT 
                 25 
                 21647 
                 21662 
                 1123 
               
               
                   
               
               
                 827392 
                 N/A 
                 N/A 
                 CGATTACAGGGATTCA 
                 15 
                 21750 
                 21765 
                 593 
               
               
                   
               
               
                 827394 
                 N/A 
                 N/A 
                 GTACGATTACAGGGAT 
                 11 
                 21753 
                 21768 
                 1125 
               
               
                   
               
               
                 827395 
                 N/A 
                 N/A 
                 AGTACGATTACAGGGA 
                 8 
                 21754 
                 21769 
                 1126 
               
               
                   
               
               
                 827396 
                 N/A 
                 N/A 
                 TAGTACGATTACAGGG 
                 29 
                 21755 
                 21770 
                 1127 
               
               
                   
               
               
                 827397 
                 N/A 
                 N/A 
                 CTAGTACGATTACAGG 
                 23 
                 21756 
                 21771 
                 1128 
               
               
                   
               
               
                 827398 
                 N/A 
                 N/A 
                 ACTAGTACGATTACAG 
                 15 
                 21757 
                 21772 
                 1129 
               
               
                   
               
               
                 827399 
                 N/A 
                 N/A 
                 CACTAGTACGATTACA 
                 39 
                 21758 
                 21773 
                 1130 
               
               
                   
               
               
                 827410 
                 N/A 
                 N/A 
                 GAATCAGTATAATGTG 
                 16 
                 22306 
                 22321 
                 595 
               
               
                   
               
               
                 827413 
                 N/A 
                 N/A 
                 CCTATGAGAATCAGTA 
                 14 
                 22313 
                 22328 
                 1133 
               
               
                   
               
               
                 827414 
                 N/A 
                 N/A 
                 GCCTATGAGAATCAGT 
                 13 
                 22314 
                 22329 
                 1134 
               
               
                   
               
               
                 827415 
                 N/A 
                 N/A 
                 CTATAGTGGCCTATGA 
                 33 
                 22322 
                 22337 
                 1135 
               
               
                   
               
               
                 827416 
                 N/A 
                 N/A 
                 GATACACACTAAGCAC 
                 22 
                 22342 
                 22357 
                 1136 
               
               
                   
               
               
                 827417 
                 N/A 
                 N/A 
                 AGATACACACTAAGCA 
                 29 
                 22343 
                 22358 
                 1137 
               
               
                   
               
               
                 827429 
                 N/A 
                 N/A 
                 CTGCCCCCATGGAAAG 
                 70 
                 22782 
                 22797 
                 597 
               
               
                   
               
               
                 827432 
                 N/A 
                 N/A 
                 GGTGAGCCCTTCGCAC 
                 3 
                 22828 
                 22843 
                 1140 
               
               
                   
               
               
                 827433 
                 N/A 
                 N/A 
                 TGAAGGAGAGGCTACA 
                 45 
                 22866 
                 22881 
                 1141 
               
               
                   
               
               
                 827434 
                 N/A 
                 N/A 
                 ATTCTAGGATGTACTG 
                 36 
                 22926 
                 22941 
                 1142 
               
               
                   
               
               
                 827435 
                 N/A 
                 N/A 
                 GTGACATACTGGTGCA 
                 3 
                 22943 
                 22958 
                 1143 
               
               
                   
               
               
                 827436 
                 N/A 
                 N/A 
                 GGGATATTCCACTGGC 
                 29 
                 22983 
                 22998 
                 1144 
               
               
                   
               
               
                 827437 
                 N/A 
                 N/A 
                 AACTAGGTGATCCGGG 
                 11 
                 22996 
                 23011 
                 1145 
               
               
                   
               
               
                 827448 
                 N/A 
                 N/A 
                 CTGCAGTAGGACTGCA 
                 111 
                 23326 
                 23341 
                 598 
               
               
                   
               
               
                 827451 
                 N/A 
                 N/A 
                 GGTGAGCACGGAGCTG 
                 14 
                 23471 
                 23486 
                 1148 
               
               
                   
               
               
                 827452 
                 N/A 
                 N/A 
                 GGAGAAAGTGTGACCA 
                 56 
                 23489 
                 23504 
                 1149 
               
               
                   
               
               
                 827453 
                 N/A 
                 N/A 
                 GAGCAGGGTTAAAGGA 
                 49 
                 23502 
                 23517 
                 1150 
               
               
                   
               
               
                 827454 
                 N/A 
                 N/A 
                 TGTCATCTAGGAGATA 
                 70 
                 23597 
                 23612 
                 1151 
               
               
                   
               
               
                 827455 
                 N/A 
                 N/A 
                 TTGCATAGATCCTGTC 
                 35 
                 23609 
                 23624 
                 1152 
               
               
                   
               
               
                 827456 
                 N/A 
                 N/A 
                 CTTGATGACAGGAGCC 
                 38 
                 23660 
                 23675 
                 1153 
               
               
                   
               
               
                 827468 
                 N/A 
                 N/A 
                 CGCCATGGAGCAAGCA 
                 67 
                 24238 
                 24253 
                 600 
               
               
                   
               
               
                 827471 
                 N/A 
                 N/A 
                 GGACTATGTGGCACCT 
                 47 
                 24342 
                 24357 
                 1156 
               
               
                   
               
               
                 827472 
                 N/A 
                 N/A 
                 TGGCAACCCCTGAGCT 
                 59 
                 24412 
                 24427 
                 1157 
               
               
                   
               
               
                 827473 
                 N/A 
                 N/A 
                 GTTCAGGAAGACCCGC 
                 65 
                 24437 
                 24452 
                 1158 
               
               
                   
               
               
                 827474 
                 N/A 
                 N/A 
                 GCAGAGGCGGGAATCC 
                 49 
                 24524 
                 24539 
                 1159 
               
               
                   
               
               
                 827475 
                 N/A 
                 N/A 
                 CATCAGGGACAGACCT 
                 43 
                 24564 
                 24579 
                 1160 
               
               
                   
               
               
                 827476 
                 N/A 
                 N/A 
                 CTGCAATCTGAGGCGC 
                 58 
                 24761 
                 24776 
                 1161 
               
               
                   
               
               
                 827488 
                 N/A 
                 N/A 
                 GGACAATTCCTTGACA 
                 36 
                 26078 
                 26093 
                 602 
               
               
                   
               
               
                 827491 
                 N/A 
                 N/A 
                 ACCTTAGGAGCCATTG 
                 18 
                 26493 
                 26508 
                 1164 
               
               
                   
               
               
                 827492 
                 N/A 
                 N/A 
                 ACCCATGTATCTTCTA 
                 44 
                 26627 
                 26642 
                 1165 
               
               
                   
               
               
                 827493 
                 N/A 
                 N/A 
                 AATGAGACAGACCCAT 
                 42 
                 26637 
                 26652 
                 1166 
               
               
                   
               
               
                 827494 
                 N/A 
                 N/A 
                 GGATACAGTATGTCCA 
                 52 
                 26685 
                 26700 
                 1167 
               
               
                   
               
               
                 827495 
                 N/A 
                 N/A 
                 CTCTACTATTGAATGG 
                 45 
                 26699 
                 26714 
                 1168 
               
               
                   
               
               
                 827496 
                 N/A 
                 N/A 
                 ATTATATACCTCTACT 
                 58 
                 26708 
                 26723 
                 1169 
               
               
                   
               
               
                 827508 
                 N/A 
                 N/A 
                 AGGTAGGGATGGACGC 
                 38 
                 27147 
                 27162 
                 604 
               
               
                   
               
               
                 827511 
                 N/A 
                 N/A 
                 CCAGGAGGCCACGACT 
                 32 
                 27241 
                 27256 
                 1172 
               
               
                   
               
               
                 827512 
                 N/A 
                 N/A 
                 TACAATCCTCTAAGGT 
                 47 
                 27271 
                 27286 
                 1173 
               
               
                   
               
               
                 827513 
                 N/A 
                 N/A 
                 CTGTATACCCTGGGAC 
                 41 
                 27378 
                 27393 
                 1174 
               
               
                   
               
               
                 827514 
                 N/A 
                 N/A 
                 TCTCAGCAATCAATAT 
                 75 
                 27490 
                 27505 
                 1175 
               
               
                   
               
               
                 827515 
                 N/A 
                 N/A 
                 GGGAAGTAAGCCCTAG 
                 22 
                 27559 
                 27574 
                 1176 
               
               
                   
               
               
                 827516 
                 N/A 
                 N/A 
                 GGCTGGAGATCTTTAG 
                 36 
                 27607 
                 27622 
                 1177 
               
               
                   
               
               
                 827528 
                 N/A 
                 N/A 
                 TGGGACTTGCTAATGA 
                 42 
                 28251 
                 28266 
                 606 
               
               
                   
               
               
                 827531 
                 N/A 
                 N/A 
                 CAGAATAGCCGGGCGC 
                 36 
                 28650 
                 28665 
                 1180 
               
               
                   
               
               
                 827532 
                 N/A 
                 N/A 
                 GGCAGACACGAGGGTC 
                 31 
                 28699 
                 28714 
                 1181 
               
               
                   
               
               
                 827533 
                 N/A 
                 N/A 
                 CCATACGGATGAACCT 
                 24 
                 28741 
                 28756 
                 1182 
               
               
                   
               
               
                 827534 
                 N/A 
                 N/A 
                 TACCATACGGATGAAC 
                 31 
                 28743 
                 28758 
                 1183 
               
               
                   
               
               
                 827535 
                 N/A 
                 N/A 
                 CTACCATACGGATGAA 
                 38 
                 28744 
                 28759 
                 1184 
               
               
                   
               
               
                 827547 
                 N/A 
                 N/A 
                 GGTGATGTCACTTCGG 
                 8 
                 29031 
                 29046 
                 608 
               
               
                   
               
               
                 827550 
                 N/A 
                 N/A 
                 AGGGAATTAAGCCACA 
                 8 
                 29501 
                 29516 
                 1187 
               
               
                   
               
               
                 827551 
                 N/A 
                 N/A 
                 GGATACACCAGTGTAA 
                 43 
                 29904 
                 29919 
                 1188 
               
               
                   
               
               
                 827552 
                 N/A 
                 N/A 
                 AGCTAAGTCAGGCGAA 
                 38 
                 29930 
                 29945 
                 1189 
               
               
                   
               
               
                 827553 
                 N/A 
                 N/A 
                 TATGAGTGTGCCTTTG 
                 42 
                 30329 
                 30344 
                 1190 
               
               
                   
               
               
                 827554 
                 N/A 
                 N/A 
                 TTCAAGGTTGCAAGTG 
                 24 
                 30348 
                 30363 
                 1191 
               
               
                   
               
               
                 827555 
                 N/A 
                 N/A 
                 AGCTAAGCCAGGGACA 
                 59 
                 30416 
                 30431 
                 1192 
               
               
                   
               
               
                 827567 
                 N/A 
                 N/A 
                 GGATAGGGTTGTGTCA 
                 96 
                 30925 
                 30940 
                 610 
               
               
                   
               
               
                 827570 
                 N/A 
                 N/A 
                 ATCAAGGTCACTCCCA 
                 65 
                 30959 
                 30974 
                 1195 
               
               
                   
               
               
                 827571 
                 N/A 
                 N/A 
                 GAAGACCCATTCCTAG 
                 69 
                 30992 
                 31007 
                 1196 
               
               
                   
               
               
                 827572 
                 N/A 
                 N/A 
                 CCATATCGATCCCTCT 
                 67 
                 31115 
                 31130 
                 1197 
               
               
                   
               
               
                 827573 
                 N/A 
                 N/A 
                 GAATTTCCTGGACCTT 
                 64 
                 31142 
                 31157 
                 1198 
               
               
                   
               
               
                 827574 
                 N/A 
                 N/A 
                 GAAATGGTAGAGGATG 
                 82 
                 31157 
                 31172 
                 1199 
               
               
                   
               
               
                 827575 
                 N/A 
                 N/A 
                 AGGCACGACCTACCGT 
                 124 
                 31272 
                 31287 
                 1200 
               
               
                   
               
            
           
         
       
     
     Example 2: Effect of Modified Oligonucleotides Complementary to α-ENaC in Hep3B Cells at Various Doses 
     Selected oligonucleotides listed in Example 1 were tested at various doses in Hep3B cells. Cells were plated at a density of 20,000 cells per well and transfected using electroporation with 148, 444, 1,333, or 4,000 nM of modified oligonucleotide, as specified in the tables below. After a treatment period of approximately 24 hours, total RNA was isolated and analyzed as described in Example 1. As illustrated in the tables below, α-ENaC mRNA levels were reduced in a dose-dependent manner in cells treated with a modified oligonucleotide complementary to an α-ENaC nucleic acid. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
            
               
                 Compound 
                 α-ENaC expression (% control) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Number 
                 148 nM 
                 444 nM 
                 1,333 nM 
                 4,000 nM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797192 
                 39 
                 30 
                 13 
                 8 
               
               
                 797235 
                 68 
                 13 
                 6 
                 5 
               
               
                 797294 
                 41 
                 25 
                 13 
                 5 
               
               
                 797495 
                 16 
                 17 
                 23 
                 12 
               
               
                 797501 
                 34 
                 21 
                 20 
                 3 
               
               
                 797507 
                 25 
                 14 
                 5 
                 8 
               
               
                 826229 
                 74 
                 17 
                 8 
                 4 
               
               
                 826249 
                 86 
                 24 
                 8 
                 6 
               
               
                 826683 
                 80 
                 64 
                 40 
                 15 
               
               
                 826761 
                 53 
                 11 
                 9 
                 5 
               
               
                 826799 
                 40 
                 26 
                 12 
                 7 
               
               
                 826800 
                 51 
                 40 
                 24 
                 11 
               
               
                 826877 
                 63 
                 49 
                 27 
                 8 
               
               
                 827277 
                 36 
                 42 
                 35 
                 10 
               
               
                 827372 
                 9 
                 14 
                 1 
                 2 
               
               
                 827392 
                 17 
                 13 
                 7 
                 3 
               
               
                 827410 
                 66 
                 39 
                 23 
                 7 
               
               
                 827449 
                 35 
                 22 
                 18 
                 5 
               
               
                 827547 
                 11 
                 5 
                 4 
                 1 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
            
               
                 Compound 
                 α-ENaC expression (% control) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Number 
                 148 nM 
                 444 nM 
                 1,333 nM 
                 4,000 nM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797469 
                 78 
                 49 
                 24 
                 16 
               
               
                 797501 
                 46 
                 29 
                 40 
                 20 
               
               
                 826232 
                 40 
                 43 
                 16 
                 7 
               
               
                 826233 
                 74 
                 49 
                 19 
                 28 
               
               
                 826626 
                 70 
                 51 
                 38 
                 9 
               
               
                 826743 
                 75 
                 43 
                 25 
                 12 
               
               
                 826763 
                 65 
                 25 
                 24 
                 7 
               
               
                 826764 
                 58 
                 43 
                 34 
                 25 
               
               
                 826784 
                 81 
                 50 
                 16 
                 15 
               
               
                 826819 
                 42 
                 26 
                 15 
                 3 
               
               
                 826821 
                 73 
                 51 
                 29 
                 10 
               
               
                 826878 
                 38 
                 32 
                 22 
                 2 
               
               
                 826880 
                 35 
                 30 
                 11 
                 17 
               
               
                 827179 
                 50 
                 28 
                 8 
                 5 
               
               
                 827199 
                 39 
                 14 
                 18 
                 9 
               
               
                 827278 
                 33 
                 22 
                 10 
                 9 
               
               
                 827393 
                 48 
                 23 
                 9 
                 6 
               
               
                 827432 
                 59 
                 49 
                 18 
                 7 
               
               
                 827550 
                 77 
                 53 
                 38 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
            
               
                 Compound 
                 α-ENaC expression (% control) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Number 
                 148 nM 
                 444 nM 
                 1,333 nM 
                 4,000 nM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797501 
                 27 
                 17 
                 10 
                 5 
               
               
                 826213 
                 35 
                 23 
                 16 
                 9 
               
               
                 826411 
                 57 
                 30 
                 23 
                 7 
               
               
                 826451 
                 80 
                 59 
                 30 
                 27 
               
               
                 826508 
                 55 
                 42 
                 25 
                 11 
               
               
                 826668 
                 34 
                 27 
                 13 
                 16 
               
               
                 826687 
                 69 
                 34 
                 29 
                 6 
               
               
                 826688 
                 43 
                 22 
                 6 
                 4 
               
               
                 826746 
                 34 
                 21 
                 21 
                 9 
               
               
                 826785 
                 39 
                 23 
                 9 
                 4 
               
               
                 827042 
                 57 
                 30 
                 18 
                 15 
               
               
                 827081 
                 37 
                 11 
                 6 
                 3 
               
               
                 827200 
                 35 
                 19 
                 11 
                 7 
               
               
                 827280 
                 19 
                 15 
                 7 
                 4 
               
               
                 827318 
                 51 
                 21 
                 11 
                 11 
               
               
                 827378 
                 44 
                 29 
                 8 
                 12 
               
               
                 827395 
                 44 
                 22 
                 19 
                 4 
               
               
                 827414 
                 54 
                 27 
                 15 
                 14 
               
               
                 827435 
                 28 
                 19 
                 7 
                 3 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
            
               
                 Compound 
                 α-ENaC expression (% control) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Number 
                 148 nM 
                 444 nM 
                 1,333 nM 
                 4,000 nM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797309 
                 53 
                 15 
                 12 
                 8 
               
               
                 797501 
                 24 
                 20 
                 16 
                 11 
               
               
                 826334 
                 65 
                 43 
                 26 
                 23 
               
               
                 826392 
                 46 
                 26 
                 25 
                 5 
               
               
                 826393 
                 57 
                 12 
                 12 
                 7 
               
               
                 826394 
                 51 
                 32 
                 16 
                 17 
               
               
                 826591 
                 44 
                 14 
                 13 
                 6 
               
               
                 826631 
                 22 
                 10 
                 9 
                 2 
               
               
                 826632 
                 38 
                 22 
                 13 
                 14 
               
               
                 826689 
                 54 
                 33 
                 17 
                 10 
               
               
                 826809 
                 21 
                 12 
                 10 
                 2 
               
               
                 826825 
                 41 
                 23 
                 18 
                 3 
               
               
                 827283 
                 46 
                 28 
                 20 
                 14 
               
               
                 827301 
                 65 
                 49 
                 24 
                 13 
               
               
                 827359 
                 18 
                 15 
                 6 
                 3 
               
               
                 827360 
                 33 
                 10 
                 15 
                 2 
               
               
                 827379 
                 28 
                 22 
                 13 
                 8 
               
               
                 827398 
                 34 
                 25 
                 16 
                 4 
               
               
                 827437 
                 37 
                 24 
                 8 
                 11 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
            
               
                 Compound 
                 α-ENaC expression (% control) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Number 
                 148 nM 
                 444 nM 
                 1,333 nM 
                 4,000 nM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797304 
                 55 
                 31 
                 17 
                 7 
               
               
                 797308 
                 28 
                 19 
                 7 
                 14 
               
               
                 797494 
                 44 
                 35 
                 14 
                 11 
               
               
                 797501 
                 56 
                 26 
                 12 
                 17 
               
               
                 826259 
                 79 
                 40 
                 19 
                 8 
               
               
                 826514 
                 54 
                 53 
                 32 
                 25 
               
               
                 826655 
                 65 
                 46 
                 32 
                 18 
               
               
                 826711 
                 51 
                 28 
                 30 
                 12 
               
               
                 826828 
                 57 
                 35 
                 18 
                 4 
               
               
                 826906 
                 72 
                 20 
                 22 
                 24 
               
               
                 827148 
                 74 
                 47 
                 34 
                 23 
               
               
                 827284 
                 34 
                 22 
                 13 
                 5 
               
               
                 827382 
                 53 
                 31 
                 27 
                 18 
               
               
                 827383 
                 69 
                 60 
                 37 
                 23 
               
               
                 827419 
                 33 
                 18 
                 13 
                 5 
               
               
                 827420 
                 71 
                 34 
                 20 
                 12 
               
               
                 827497 
                 46 
                 14 
                 11 
                 9 
               
               
                 827498 
                 56 
                 34 
                 24 
                 13 
               
               
                 827518 
                 65 
                 33 
                 15 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
            
               
                 Compound 
                 α-ENaC expression (% control) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Number 
                 148 nM 
                 444 nM 
                 1,333 nM 
                 4,000 nM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797501 
                 44 
                 33 
                 16 
                 6 
               
               
                 826183 
                 57 
                 38 
                 13 
                 10 
               
               
                 826202 
                 77 
                 35 
                 25 
                 5 
               
               
                 826241 
                 43 
                 36 
                 25 
                 18 
               
               
                 826262 
                 81 
                 50 
                 21 
                 12 
               
               
                 826338 
                 79 
                 35 
                 13 
                 5 
               
               
                 826558 
                 80 
                 53 
                 24 
                 16 
               
               
                 826576 
                 70 
                 45 
                 27 
                 12 
               
               
                 826673 
                 88 
                 40 
                 29 
                 12 
               
               
                 826753 
                 64 
                 39 
                 21 
                 23 
               
               
                 826754 
                 67 
                 47 
                 22 
                 11 
               
               
                 826793 
                 62 
                 35 
                 13 
                 6 
               
               
                 826811 
                 85 
                 42 
                 18 
                 3 
               
               
                 827030 
                 43 
                 23 
                 9 
                 4 
               
               
                 827149 
                 42 
                 38 
                 23 
                 11 
               
               
                 827150 
                 54 
                 41 
                 20 
                 10 
               
               
                 827307 
                 46 
                 23 
                 20 
                 6 
               
               
                 827347 
                 66 
                 41 
                 26 
                 16 
               
               
                 827441 
                 30 
                 13 
                 11 
                 1 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC 
               
            
           
           
               
               
               
            
               
                 Compound 
                 α-ENaC expression (% control) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Number 
                 148 nM 
                 444 nM 
                 1,333 nM 
                 4,000 nM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797131 
                 55 
                 27 
                 15 
                 7 
               
               
                 797497 
                 56 
                 31 
                 28 
                 13 
               
               
                 797501 
                 34 
                 23 
                 24 
                 5 
               
               
                 826659 
                 50 
                 40 
                 13 
                 5 
               
               
                 826678 
                 44 
                 25 
                 23 
                 9 
               
               
                 826776 
                 71 
                 34 
                 24 
                 9 
               
               
                 826794 
                 52 
                 25 
                 18 
                 9 
               
               
                 826891 
                 50 
                 32 
                 18 
                 9 
               
               
                 827131 
                 100 
                 63 
                 44 
                 10 
               
               
                 827151 
                 32 
                 29 
                 12 
                 10 
               
               
                 827270 
                 54 
                 33 
                 23 
                 11 
               
               
                 827288 
                 42 
                 35 
                 20 
                 5 
               
               
                 827289 
                 65 
                 33 
                 21 
                 7 
               
               
                 827309 
                 79 
                 45 
                 30 
                 7 
               
               
                 827348 
                 69 
                 54 
                 33 
                 10 
               
               
                 827368 
                 63 
                 35 
                 22 
                 7 
               
               
                 827386 
                 85 
                 46 
                 19 
                 6 
               
               
                 827502 
                 55 
                 21 
                 12 
                 11 
               
               
                 827524 
                 78 
                 39 
                 26 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC 
               
            
           
           
               
               
               
            
               
                 Compound 
                 α-ENaC expression (% control) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Number 
                 148 nM 
                 444 nM 
                 1,333 nM 
                 4,000 nM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797264 
                 59 
                 30 
                 28 
                 5 
               
               
                 797312 
                 63 
                 44 
                 28 
                 11 
               
               
                 797501 
                 47 
                 12 
                 6 
                 3 
               
               
                 826168 
                 87 
                 58 
                 16 
                 11 
               
               
                 826169 
                 65 
                 29 
                 15 
                 13 
               
               
                 826403 
                 66 
                 35 
                 18 
                 13 
               
               
                 826484 
                 65 
                 46 
                 23 
                 11 
               
               
                 826660 
                 60 
                 53 
                 22 
                 7 
               
               
                 826679 
                 60 
                 46 
                 35 
                 14 
               
               
                 826718 
                 68 
                 51 
                 40 
                 6 
               
               
                 826796 
                 66 
                 61 
                 36 
                 9 
               
               
                 826816 
                 104 
                 53 
                 26 
                 10 
               
               
                 827035 
                 57 
                 28 
                 25 
                 9 
               
               
                 827134 
                 112 
                 57 
                 31 
                 33 
               
               
                 827175 
                 43 
                 39 
                 13 
                 7 
               
               
                 827254 
                 53 
                 36 
                 24 
                 6 
               
               
                 827408 
                 53 
                 28 
                 21 
                 15 
               
               
                 827426 
                 79 
                 34 
                 23 
                 9 
               
               
                 827447 
                 35 
                 18 
                 13 
                 10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC 
               
            
           
           
               
               
               
            
               
                 Compound 
                 α-ENaC expression (% control) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Number 
                 148 nM 
                 444 nM 
                 1,333 nM 
                 4,000 nM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797469 
                 81 
                 55 
                 24 
                 17 
               
               
                 797501 
                 26 
                 25 
                 41 
                 21 
               
               
                 826232 
                 39 
                 32 
                 14 
                 5 
               
               
                 826233 
                 88 
                 58 
                 23 
                 29 
               
               
                 826626 
                 59 
                 53 
                 39 
                 8 
               
               
                 826743 
                 83 
                 47 
                 23 
                 10 
               
               
                 826763 
                 77 
                 24 
                 20 
                 9 
               
               
                 826764 
                 33 
                 45 
                 37 
                 26 
               
               
                 826784 
                 44 
                 47 
                 16 
                 15 
               
               
                 826819 
                 40 
                 24 
                 10 
                 2 
               
               
                 826821 
                 81 
                 52 
                 31 
                 11 
               
               
                 826878 
                 39 
                 31 
                 19 
                 2 
               
               
                 826880 
                 41 
                 28 
                 13 
                 17 
               
               
                 827179 
                 62 
                 24 
                 7 
                 5 
               
               
                 827199 
                 44 
                 14 
                 20 
                 10 
               
               
                 827278 
                 31 
                 23 
                 10 
                 9 
               
               
                 827393 
                 55 
                 22 
                 9 
                 6 
               
               
                 827432 
                 66 
                 52 
                 24 
                 7 
               
               
                 827550 
                 62 
                 43 
                 36 
                 16 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 15 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC 
               
            
           
           
               
               
               
            
               
                 Compound 
                 α-ENaC expression (% control) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Number 
                 148 nM 
                 444 nM 
                 1,333 nM 
                 4,000 nM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797131 
                 58 
                 28 
                 16 
                 7 
               
               
                 797497 
                 61 
                 34 
                 30 
                 14 
               
               
                 797501 
                 37 
                 24 
                 26 
                 5 
               
               
                 826659 
                 54 
                 42 
                 14 
                 5 
               
               
                 826678 
                 47 
                 26 
                 25 
                 9 
               
               
                 826776 
                 76 
                 36 
                 26 
                 10 
               
               
                 826794 
                 57 
                 28 
                 19 
                 10 
               
               
                 826891 
                 54 
                 34 
                 19 
                 10 
               
               
                 827131 
                 109 
                 69 
                 48 
                 10 
               
               
                 827151 
                 34 
                 32 
                 13 
                 11 
               
               
                 827270 
                 57 
                 35 
                 25 
                 11 
               
               
                 827288 
                 45 
                 37 
                 21 
                 6 
               
               
                 827289 
                 71 
                 36 
                 23 
                 8 
               
               
                 827309 
                 84 
                 48 
                 32 
                 8 
               
               
                 827348 
                 73 
                 58 
                 35 
                 11 
               
               
                 827368 
                 67 
                 38 
                 23 
                 7 
               
               
                 827386 
                 92 
                 50 
                 21 
                 7 
               
               
                 827502 
                 60 
                 24 
                 13 
                 12 
               
               
                 827524 
                 83 
                 41 
                 28 
                 15 
               
               
                   
               
            
           
         
       
     
     Example 3: Effect of Various Doses of Modified Oligonucleotides Complementary to Human α-ENaC In Vitro Via Free Uptake 
     Selected oligonucleotides were tested at various doses in A431 cells by free uptake. Cells were plated at a density of 10,000 cells per well with 16, 49, 148, 1,333, or 4,000 nM of modified oligonucleotide, as specified in the tables below. After a treatment period of approximately 24 hours, total RNA was isolated and analyzed as in Example 1. As illustrated in the tables below, α-ENaC mRNA levels were reduced in a dose-dependent manner in cells treated with a modified oligonucleotide complementary to an α-ENaC nucleic acid. 
     
       
         
           
               
             
               
                 TABLE 16 
               
             
            
               
                   
               
               
                 Level of α-ENaC mRNA in A431 cells 
               
            
           
           
               
               
            
               
                   
                 α-ENaC expression (% control) 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Compound 
                 16 
                 49 
                 148 
                 444 
                 1,333 
                 4,000 
                 IC50 
               
               
                 Number 
                 nM 
                 nM 
                 nM 
                 nM 
                 nM 
                 nM 
                 (μM) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 797236 
                 129 
                 92 
                 50 
                 31 
                 18 
                 6 
                 0.23 
               
               
                 797308 
                 89 
                 66 
                 27 
                 13 
                 9 
                 4 
                 0.08 
               
               
                 797313 
                 94 
                 82 
                 47 
                 25 
                 15 
                 9 
                 0.17 
               
               
                 797468 
                 90 
                 77 
                 55 
                 30 
                 19 
                 11 
                 0.19 
               
               
                 797495 
                 50 
                 26 
                 11 
                 3 
                 8 
                 7 
                 0.01 
               
               
                 826632 
                 76 
                 75 
                 61 
                 28 
                 22 
                 11 
                 0.22 
               
               
                 826743 
                 85 
                 81 
                 57 
                 28 
                 23 
                 19 
                 0.22 
               
               
                 826763 
                 73 
                 55 
                 35 
                 16 
                 14 
                 8 
                 0.06 
               
               
                 826819 
                 85 
                 87 
                 73 
                 58 
                 44 
                 38 
                 1.06 
               
               
                 826906 
                 85 
                 75 
                 52 
                 30 
                 17 
                 9 
                 0.16 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 17 
               
             
            
               
                   
               
               
                 Level of α-ENaC mRNA in A431 cells 
               
            
           
           
               
               
            
               
                   
                 α-ENaC expression (% control) 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Compound 
                 16 
                 49 
                 148 
                 444 
                 1,333 
                 4,000 
                 IC50 
               
               
                 Number 
                 nM 
                 nM 
                 nM 
                 nM 
                 nM 
                 nM 
                 (μM) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 827030 
                 109 
                 98 
                 78 
                 55 
                 41 
                 35 
                 0.97 
               
               
                 827200 
                 100 
                 85 
                 79 
                 55 
                 50 
                 38 
                 1.23 
               
               
                 827288 
                 85 
                 71 
                 53 
                 33 
                 13 
                 21 
                 0.16 
               
               
                 827307 
                 68 
                 58 
                 33 
                 14 
                 5 
                 2 
                 0.06 
               
               
                 827347 
                 66 
                 47 
                 18 
                 5 
                 2 
                 1 
                 0.04 
               
               
                 827359 
                 56 
                 45 
                 27 
                 11 
                 6 
                 4 
                 0.03 
               
               
                 827372 
                 47 
                 22 
                 7 
                 3 
                 1 
                 1 
                 0.01 
               
               
                 827392 
                 76 
                 44 
                 18 
                 7 
                 4 
                 3 
                 0.04 
               
               
                 827414 
                 79 
                 60 
                 38 
                 17 
                 8 
                 5 
                 0.08 
               
               
                 827497 
                 64 
                 49 
                 24 
                 8 
                 6 
                 5 
                 0.04 
               
               
                   
               
            
           
         
       
     
     Example 4: Tolerability of Modified Oligonucleotides Complementary to Human α-ENaC in CD1 Mice Following Systemic Delivery 
     CD1® mice (Charles River, Mass.) are a multipurpose mice model, frequently utilized for safety and efficacy testing. The mice were treated with modified oligonucleotides selected from studies described above and evaluated for changes in the levels of various plasma chemistry markers. 
     Treatment 
     Groups of 6-8 week old male CD1 mice were injected subcutaneously once a week for 6 weeks with 50 mg/kg of a modified oligonucleotide listed in the tables below (50 mg/kg/week dose). Each group contained 4 mice. One group of male CD1 mice was injected subcutaneously once a week for 6 weeks with PBS. Mice were sacrificed 48 hours after the last dose, and organs and plasma were harvested for further analysis. 
     Plasma Chemistry Markers 
     To evaluate the effect of modified oligonucleotides on liver and kidney function, plasma levels of transaminases, albumin, BUN, and billirubin were measured using an automated clinical chemistry analyzer (Hitachi Olympus AU400e, Melville, N.Y.). The results in the tables below show that most of the tested modified oligonucleotides were well tolerated when delivered systemically, with ALT and AST levels under approximately 200 IU/L and albumin, BUN, creatine, and total bilirubin within acceptable ranges. 
     
       
         
           
               
             
               
                 TABLE 18 
               
             
            
               
                   
               
               
                 Levels of plasma chemistry markers 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Compound 
                 ALT 
                 AST 
                   
                   
                   
                   
               
               
                 No. 
                 (IU/L) 
                 (IU/L) 
                 Albumin 
                 BUN 
                 Creatine 
                 T. Bil. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 PBS 
                 34.8 
                 39.8 
                 2.83 
                 25.0 
                 .065 
                 .195 
               
               
                 797131 
                 167.8 
                 162.0 
                 2.39 
                 21.6 
                 .055 
                 .188 
               
               
                 797236 
                 70.5 
                 93.8 
                 2.78 
                 24.8 
                 .057 
                 .183 
               
               
                 797258 
                 1061.3 
                 1077.8 
                 2.91 
                 25.8 
                 .060 
                 .250 
               
               
                 797262 
                 244.5 
                 324.0 
                 2.54 
                 27.9 
                 .045 
                 .165 
               
               
                 797264 
                 484.0 
                 247.3 
                 2.92 
                 26.1 
                 .080 
                 .178 
               
               
                 797266 
                 641.3 
                 330.3 
                 3.05 
                 24.7 
                 .070 
                 .180 
               
               
                 797289 
                 218.8 
                 175.5 
                 2.66 
                 21.8 
                 .065 
                 .148 
               
               
                 797293 
                 921.5 
                 638.5 
                 2.85 
                 27.1 
                 .080 
                 .208 
               
               
                 797294 
                 248.5 
                 226.0 
                 2.99 
                 23.1 
                 .043 
                 .268 
               
               
                 797295 
                 1262.8 
                 954.3 
                 3.01 
                 22.7 
                 .063 
                 .408 
               
               
                 797304 
                 252.3 
                 208.8 
                 2.70 
                 23.0 
                 .060 
                 .208 
               
               
                 797307 
                 151.3 
                 123.8 
                 2.97 
                 25.6 
                 .093 
                 .198 
               
               
                 797308 
                 65.8 
                 114.3 
                 2.71 
                 22.8 
                 .070 
                 .158 
               
               
                 797312 
                 1630.5 
                 862.8 
                 3.40 
                 25.2 
                 .135 
                 .315 
               
               
                 797313 
                 46.3 
                 77.0 
                 3.15 
                 23.4 
                 .140 
                 .203 
               
               
                 797340 
                 558.3 
                 316.0 
                 3.26 
                 28.6 
                 .143 
                 .263 
               
               
                 797444 
                 224.0 
                 285.8 
                 2.23 
                 27.6 
                 .048 
                 .205 
               
               
                 797466 
                 433.5 
                 446.3 
                 2.79 
                 22.4 
                 .093 
                 .138 
               
               
                 797468 
                 87.0 
                 101.8 
                 2.94 
                 23.4 
                 .120 
                 .145 
               
               
                 797495 
                 45.8 
                 121.8 
                 3.62 
                 24.0 
                 .148 
                 .263 
               
               
                 797497 
                 61.0 
                 78.0 
                 2.95 
                 24.3 
                 .125 
                 .148 
               
               
                 797500 
                 43.8 
                 59.3 
                 3.11 
                 24.2 
                 .118 
                 .193 
               
               
                 797501 
                 546.0 
                 500.8 
                 2.82 
                 25.6 
                 .115 
                 .303 
               
               
                 797507 
                 65.5 
                 89.8 
                 3.03 
                 22.8 
                 .090 
                 .168 
               
               
                 797508 
                 120.5 
                 188.3 
                 2.41 
                 23.9 
                 .055 
                 .155 
               
               
                 797523 
                 237.3 
                 168.8 
                 3.48 
                 25.4 
                 .110 
                 .170 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 19 
               
             
            
               
                   
               
               
                 Levels of plasma chemistry markers 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Compound 
                 ALT 
                 AST 
                   
                   
                   
                   
               
               
                 No. 
                 (IU/L) 
                 (IU/L) 
                 Albumin 
                 BUN 
                 Creatine 
                 T. Bil. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 PBS 
                 54.0 
                 59.5 
                 2.81 
                 25.8 
                 .080 
                 .193 
               
               
                 797192 
                 2079.8 
                 889.5 
                 3.26 
                 25.2 
                 .125 
                 .793 
               
               
                 797309 
                 112.5 
                 91.5 
                 2.91 
                 22.5 
                 .078 
                 .165 
               
               
                 797469 
                 3698.3 
                 3053.3 
                 3.80 
                 20.1 
                 .148 
                 .188 
               
               
                 826183 
                 n.d. 
                 n.d. 
                 n.d. 
                 n.d. 
                 n.d. 
                 n.d. 
               
               
                 826262 
                 236.8 
                 196.8 
                 2.45 
                 21.9 
                 .058 
                 .195 
               
               
                 826393 
                 116.8 
                 89.8 
                 2.85 
                 20.5 
                 .070 
                 .168 
               
               
                 826394 
                 466.0 
                 196.3 
                 2.81 
                 23.1 
                 .068 
                 .218 
               
               
                 826558 
                 126.8 
                 117.3 
                 2.97 
                 21.6 
                 .080 
                 .155 
               
               
                 826631 
                 1073.5 
                 1267.0 
                 2.61 
                 19.0 
                 .040 
                 .275 
               
               
                 826632 
                 113.3 
                 129.5 
                 2.94 
                 22.4 
                 .085 
                 .173 
               
               
                 826655 
                 1349.8 
                 754.5 
                 3.13 
                 21.1 
                 .123 
                 6.315 
               
               
                 826687 
                 1174.0 
                 647.0 
                 2.85 
                 20.0 
                 .075 
                 .178 
               
               
                 826688 
                 430.0 
                 362.0 
                 3.38 
                 22.5 
                 .100 
                 .215 
               
               
                 826743 
                 115.0 
                 111.8 
                 2.94 
                 21.6 
                 .085 
                 .160 
               
               
                 826753 
                 58.0 
                 70.0 
                 2.68 
                 24.5 
                 .070 
                 .138 
               
               
                 826763 
                 77.0 
                 89.3 
                 2.97 
                 22.9 
                 .083 
                 .203 
               
               
                 826776 
                 1685.8 
                 1027.0 
                 2.55 
                 20.7 
                 .058 
                 .248 
               
               
                 826793 
                 1306.3 
                 563.5 
                 2.85 
                 18.8 
                 .065 
                 .250 
               
               
                 826796 
                 1552.5 
                 740.8 
                 3.29 
                 19.5 
                 .088 
                 .453 
               
               
                 826811 
                 439.3 
                 347.5 
                 3.00 
                 19.5 
                 .085 
                 .438 
               
               
                 826819 
                 297.0 
                 146.8 
                 2.88 
                 26.6 
                 .125 
                 .163 
               
               
                 826828 
                 559.0 
                 314.0 
                 2.71 
                 17.4 
                 .060 
                 .170 
               
               
                 826906 
                 41.0 
                 56.5 
                 2.98 
                 22.5 
                 .093 
                 .163 
               
               
                 827030 
                 44.0 
                 55.8 
                 3.04 
                 20.0 
                 .093 
                 .180 
               
               
                 827035 
                 n.d. 
                 n.d. 
                 n.d. 
                 n.d. 
                 n.d. 
                 n.d. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 20 
               
             
            
               
                   
               
               
                 Levels of plasma chemistry markers 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Compound 
                 ALT 
                 AST 
                   
                   
                   
                   
               
               
                 No. 
                 (IU/L) 
                 (IU/L) 
                 Albumin 
                 BUN 
                 Creatine 
                 T. Bil. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 PBS 
                 45.0 
                 53.0 
                 2.80 
                 25.5 
                 .065 
                 .255 
               
               
                 827148 
                 33.5 
                 52.3 
                 2.63 
                 23.8 
                 .080 
                 .223 
               
               
                 827150 
                 n.d. 
                 n.d. 
                 n.d. 
                 n.d. 
                 n.d. 
                 n.d. 
               
               
                 827175 
                 123.8 
                 246.8 
                 2.50 
                 26.1 
                 .078 
                 .218 
               
               
                 827200 
                 61.5 
                 51.0 
                 2.79 
                 27.1 
                 .058 
                 .195 
               
               
                 827254 
                 155.3 
                 161.5 
                 2.77 
                 26.9 
                 .068 
                 .265 
               
               
                 827288 
                 65.8 
                 59.5 
                 2.77 
                 22.5 
                 .068 
                 .280 
               
               
                 827307 
                 55.5 
                 61.0 
                 2.70 
                 26.3 
                 .053 
                 .203 
               
               
                 827347 
                 52.5 
                 60.0 
                 2.72 
                 25.9 
                 .063 
                 .140 
               
               
                 827348 
                 284.5 
                 197.5 
                 2.99 
                 21.9 
                 .070 
                 .330 
               
               
                 827359 
                 65.8 
                 72.8 
                 2.60 
                 22.7 
                 .063 
                 .210 
               
               
                 827360 
                 45.8 
                 52.8 
                 2.68 
                 24.1 
                 .088 
                 .188 
               
               
                 827372 
                 49.0 
                 51.5 
                 2.73 
                 24.7 
                 .053 
                 .213 
               
               
                 827382 
                 33.3 
                 45.8 
                 2.69 
                 22.0 
                 .048 
                 .183 
               
               
                 827392 
                 39.5 
                 57.0 
                 2.77 
                 21.3 
                 .063 
                 .163 
               
               
                 827393 
                 207.5 
                 118.8 
                 2.82 
                 24.2 
                 .050 
                 .220 
               
               
                 827398 
                 121.3 
                 181.3 
                 2.66 
                 22.8 
                 .045 
                 .178 
               
               
                 827408 
                 339.8 
                 292.5 
                 2.53 
                 28.0 
                 .048 
                 .143 
               
               
                 827410 
                 205.8 
                 436.8 
                 2.66 
                 19.7 
                 .040 
                 .163 
               
               
                 827414 
                 145.8 
                 123.0 
                 2.59 
                 23.2 
                 .055 
                 .148 
               
               
                 827419 
                 62.0 
                 73.3 
                 2.68 
                 27.5 
                 .065 
                 .178 
               
               
                 827437 
                 63.8 
                 103.8 
                 2.56 
                 19.5 
                 .050 
                 .243 
               
               
                 827449 
                 300.0 
                 211.3 
                 2.49 
                 22.0 
                 .040 
                 .168 
               
               
                 827497 
                 100.5 
                 112.8 
                 2.19 
                 22.0 
                 .043 
                 .170 
               
               
                 827502 
                 126.3 
                 106.3 
                 2.36 
                 22.9 
                 .043 
                 .108 
               
               
                   
               
            
           
         
       
     
     Organ Weights 
     Organ weights were measured at the end of the study, and kidney, liver, and spleen weights are presented in the table below. The results provide additional evidence that most of the modified oligonucleotides were well tolerated when delivered systemically. 
     
       
         
           
               
             
               
                 TABLE 21 
               
             
            
               
                   
               
               
                 Organ weights 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Compound No. 
                 Kidney (g) 
                 Liver (g) 
                 Spleen (g) 
               
               
                   
                   
               
               
                   
                 PBS 
                 0.678 
                 2.542 
                 0.133 
               
               
                   
                 797131 
                 0.598 
                 2.263 
                 0.151 
               
               
                   
                 797236 
                 0.621 
                 2.281 
                 0.195 
               
               
                   
                 797258 
                 0.719 
                 3.663 
                 0.212 
               
               
                   
                 797262 
                 0.625 
                 3.214 
                 0.127 
               
               
                   
                 797264 
                 0.496 
                 2.485 
                 0.169 
               
               
                   
                 797266 
                 0.547 
                 2.633 
                 0.169 
               
               
                   
                 797289 
                 0.618 
                 3.064 
                 0.165 
               
               
                   
                 797293 
                 0.647 
                 2.197 
                 0.200 
               
               
                   
                 797294 
                 0.566 
                 1.675 
                 0.154 
               
               
                   
                 797295 
                 0.558 
                 2.147 
                 0.170 
               
               
                   
                 797304 
                 0.691 
                 3.263 
                 0.173 
               
               
                   
                 797307 
                 0.607 
                 2.546 
                 0.203 
               
               
                   
                 797308 
                 0.616 
                 2.113 
                 0.143 
               
               
                   
                 797312 
                 0.562 
                 2.941 
                 0.123 
               
               
                   
                 797313 
                 0.536 
                 2.236 
                 0.138 
               
               
                   
                 797340 
                 0.529 
                 2.325 
                 0.144 
               
               
                   
                 797444 
                 0.580 
                 6.846 
                 0.551 
               
               
                   
                 797466 
                 0.491 
                 2.289 
                 0.222 
               
               
                   
                 797468 
                 0.617 
                 2.275 
                 0.145 
               
               
                   
                 797495 
                 0.602 
                 2.300 
                 0.128 
               
               
                   
                 797497 
                 0.651 
                 2.483 
                 0.151 
               
               
                   
                 797500 
                 0.563 
                 2.031 
                 0.119 
               
               
                   
                 797501 
                 0.479 
                 2.978 
                 0.140 
               
               
                   
                 797507 
                 0.551 
                 2.359 
                 0.115 
               
               
                   
                 797508 
                 0.469 
                 1.322 
                 0.148 
               
               
                   
                 797523 
                 0.560 
                 2.614 
                 0.172 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 22 
               
             
            
               
                   
               
               
                 Organ weights 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Compound No. 
                 Kidney (g) 
                 Liver (g) 
                 Spleen (g) 
               
               
                   
                   
               
               
                   
                 PBS 
                 0.610 
                 2.212 
                 0.138 
               
               
                   
                 797192 
                 0.530 
                 4.632 
                 0.157 
               
               
                   
                 797309 
                 0.586 
                 2.160 
                 0.115 
               
               
                   
                 797469 
                 0.633 
                 4.636 
                 0.238 
               
               
                   
                 826183 
                 n.d. 
                 n.d. 
                 n.d. 
               
               
                   
                 826262 
                 0.609 
                 2.197 
                 0.227 
               
               
                   
                 826393 
                 0.508 
                 3.084 
                 0.210 
               
               
                   
                 826394 
                 0.530 
                 2.914 
                 0.206 
               
               
                   
                 826558 
                 0.567 
                 2.048 
                 0.149 
               
               
                   
                 826631 
                 0.587 
                 2.361 
                 0.169 
               
               
                   
                 826632 
                 0.595 
                 2.442 
                 0.136 
               
               
                   
                 826655 
                 0.588 
                 3.511 
                 0.113 
               
               
                   
                 826687 
                 0.619 
                 2.750 
                 0.261 
               
               
                   
                 826688 
                 0.546 
                 2.418 
                 0.214 
               
               
                   
                 826743 
                 0.608 
                 2.162 
                 0.110 
               
               
                   
                 826753 
                 0.538 
                 2.364 
                 0.140 
               
               
                   
                 826763 
                 0.542 
                 2.478 
                 0.144 
               
               
                   
                 826776 
                 0.574 
                 4.112 
                 0.386 
               
               
                   
                 826793 
                 0.555 
                 2.295 
                 0.173 
               
               
                   
                 826796 
                 0.605 
                 2.566 
                 0.151 
               
               
                   
                 826811 
                 0.557 
                 2.085 
                 0.133 
               
               
                   
                 826819 
                 0.517 
                 2.559 
                 0.144 
               
               
                   
                 826828 
                 0.590 
                 2.046 
                 0.191 
               
               
                   
                 826906 
                 0.561 
                 2.121 
                 0.123 
               
               
                   
                 827030 
                 0.564 
                 1.974 
                 0.114 
               
               
                   
                 827035 
                 n.d. 
                 n.d. 
                 n.d. 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 23 
               
             
            
               
                   
               
               
                 Organ weights 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Compound No. 
                 Kidney (g) 
                 Liver (g) 
                 Spleen (g) 
               
               
                   
                   
               
               
                   
                 PBS 
                 0.615 
                 2.172 
                 0.108 
               
               
                   
                 827148 
                 0.623 
                 2.413 
                 0.142 
               
               
                   
                 827150 
                 n.d. 
                 n.d. 
                 n.d. 
               
               
                   
                 827175 
                 0.683 
                 2.521 
                 0.139 
               
               
                   
                 827200 
                 0.640 
                 2.682 
                 0.127 
               
               
                   
                 827254 
                 0.631 
                 2.589 
                 0.139 
               
               
                   
                 827288 
                 0.579 
                 2.341 
                 0.138 
               
               
                   
                 827307 
                 0.614 
                 2.391 
                 0.133 
               
               
                   
                 827347 
                 0.596 
                 2.235 
                 0.152 
               
               
                   
                 827348 
                 0.678 
                 2.832 
                 0.251 
               
               
                   
                 827359 
                 0.647 
                 2.316 
                 0.146 
               
               
                   
                 827360 
                 0.517 
                 2.098 
                 0.147 
               
               
                   
                 827372 
                 0.657 
                 2.120 
                 0.140 
               
               
                   
                 827382 
                 0.574 
                 2.089 
                 0.142 
               
               
                   
                 827392 
                 0.595 
                 2.208 
                 0.124 
               
               
                   
                 827393 
                 0.603 
                 2.307 
                 0.137 
               
               
                   
                 827398 
                 0.590 
                 2.249 
                 0.141 
               
               
                   
                 827408 
                 0.751 
                 2.399 
                 0.290 
               
               
                   
                 827410 
                 0.653 
                 3.247 
                 0.174 
               
               
                   
                 827414 
                 0.663 
                 2.787 
                 0.185 
               
               
                   
                 827419 
                 0.682 
                 2.327 
                 0.150 
               
               
                   
                 827437 
                 0.674 
                 2.523 
                 0.544 
               
               
                   
                 827449 
                 0.619 
                 2.798 
                 0.155 
               
               
                   
                 827497 
                 0.630 
                 2.368 
                 0.189 
               
               
                   
                 827502 
                 0.674 
                 3.082 
                 0.183 
               
               
                   
                   
               
            
           
         
       
     
     Example 5: Establishment of a Transgenic Mouse Line Expressing Human α-ENaC 
     A transgenic mouse was developed to analyze knockdown of human α-ENaC in a mouse model. A 41,279 bp portion of the gene for human α-ENaC ABC14-50929300K14 (digested with NotI) was microinjected into embryos of C57BL/6 WT mice. Five transgene positive FO mouse pups were obtained, and one founder was used to generate a C57BL/6 ha-ENaC mouse line. The line was evaluated for expression of ha-ENaC in tongue, brain, heart, colon, trachea, pancreas, kidney, liver, spleen, skeletal muscle, fat, uterus, and both total lung and lung fractions. The mouse model exhibits ha-ENaC expression in a variety of tissues, and, importantly, high levels of expression in all fractions of the lung. 
     Example 6: Effect of Modified Oligonucleotides on Human α-ENaC Expression in a Transgenic Mouse 
     Treatment 
     Transgenic mice were maintained on a 12-hour light/dark cycle and were fed ad libitum normal diet. Animals were acclimated for at least 7 days in the research facility before initiation of the experiment. Modified oligonucleotides were prepared in buffered saline (PBS) and sterilized by filtering through a 0.2 micron filter. Oligonucleotides were dissolved in 0.9% PBS for injection. 
     The C57B1/6-TG(ha-ENaC) mice weighing ˜20 g were divided into groups of 2-4 mice. Groups of mice were administered 2.5 mg/kg of modified oligonucleotide twice a week for two weeks (5 mg/kg/week) via oropharyngeal aspiration. A control group of 6 mice was given PBS twice per week for two weeks. The PBS group served as the control group to which animals dosed with modified oligonucleotide were compared. Mice were sacrificed 48 hrs after the last dose and organs were harvested for further analysis. 
     Human α-ENaC Expression Levels 
     Total RNA was isolated from the whole lung and human α-ENaC mRNA levels were measured as described in Example 1. Results are presented in the table below as percent reduction of the amount of α-ENaC mRNA relative to untreated control. As illustrated in the table below, α-ENaC mRNA levels were reduced in lung of modified oligonucleotide-treated animals. 
     
       
         
           
               
             
               
                 TABLE 24 
               
             
            
               
                   
               
               
                 Percent level of human α-ENaC mRNA 
               
            
           
           
               
               
               
            
               
                 Compound 
                 Tissue 
                   
               
            
           
           
               
               
               
               
               
            
               
                 No. 
                 Lung 
                 Liver 
                 Colon 
                 Kidney 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 797236 
                 41 
                 62 
                 85 
                 87 
               
               
                  797308* 
                 36 
                 41 
                 99 
                 87 
               
               
                 797313 
                 41 
                 54 
                 87 
                 89 
               
               
                 797468 
                 45 
                 64 
                 77 
                 102 
               
               
                 797495 
                 27 
                 30 
                 69 
                 66 
               
               
                  826632** 
                 40 
                 81 
                 77 
                 87 
               
               
                 826743 
                 46 
                 73 
                 106 
                 101 
               
               
                 826763 
                 32 
                 51 
                 94 
                 96 
               
               
                 826819 
                 45 
                 50 
                 93 
                 86 
               
               
                 826906 
                 42 
                 64 
                 107 
                 101 
               
               
                 827030 
                 45 
                 59 
                 90 
                 73 
               
               
                 827200 
                 51 
                 72 
                 101 
                 129 
               
               
                 827288 
                 54 
                 66 
                 105 
                 75 
               
               
                 827307 
                 34 
                 68 
                 85 
                 91 
               
               
                 827347 
                 28 
                 66 
                 97 
                 103 
               
               
                  827359* 
                 34 
                 37 
                 82 
                 90 
               
               
                 827372 
                 21 
                 29 
                 50 
                 70 
               
               
                 827392 
                 28 
                 50 
                 73 
                 72 
               
               
                 827414 
                 36 
                 45 
                 84 
                 93 
               
               
                 827497 
                 34 
                 61 
                 90 
                 86 
               
               
                   
               
               
                 *Group contained 3 mice 
               
               
                 **Group contained 2 mice 
               
               
                 All other groups contained 4 mice 
               
            
           
         
       
     
     Example 7: Dose-Response of Compound 827359 on Human α-ENaC Expression in a Transgenic Mouse 
     Treatment 
     Transgenic mice were maintained on a 12-hour light/dark cycle and were fed ad libitum normal diet. Animals were acclimated for at least 7 days in the research facility before initiation of the experiment. Modified oligonucleotides were prepared in buffered saline (PBS) and sterilized by filtering through a 0.2 micron filter. Oligonucleotides were dissolved in 0.9% PBS. 
     The C57B1/6-TG(ha-ENaC) mice weighing ˜20 g were divided into groups of 12 mice. Groups of 12 mice were administered 0.033, 0.1, 0.33 or 1.0 mg/kg of modified oligonucleotide twice a week for three weeks (5 mg/kg/week) via aerosol dosing. A control group of 12 mice was given aerosol saline twice per week for 3 weeks. The PBS group served as the control group to which animals dosed with modified oligonucleotide were compared. Mice were sacrificed 3 days after the last dose and organs were harvested for further analysis. 
     Human α-ENaC Expression Levels 
     Total RNA was isolated from the whole lung and human α-ENaC mRNA levels were measured by quantitative real-time PCR as described in Example 1. Results are presented in the table below as percent reduction of the amount of α-ENaC mRNA relative to untreated control. As illustrated in the table below, α-ENaC mRNA levels were reduced in a dose-dependent manner in modified oligonucleotide-treated animals. 
     
       
         
           
               
             
               
                 TABLE 25 
               
             
            
               
                   
               
               
                 Dose Response of 827359 in transgenic mouse 
               
            
           
           
               
               
               
            
               
                   
                 Conc. 827359 
                 % 
               
               
                   
                 (mg/kg/dose) 
                 Control 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 0 [Saline] 
                 100.0 
               
               
                   
                 0.033 
                 73.4 
               
               
                   
                 0.100 
                 50.4 
               
               
                   
                 0.330 
                 38.1 
               
               
                   
                 1.000 
                 33.3 
               
               
                   
                   
               
            
           
         
       
     
     Example 8: Human Peripheral Blood Mononuclear Cells (hPBMC) Assay 
     The hPBMC assay was performed using BD Vautainer CPT tube method. A sample of whole blood from volunteered donors with informed consent at US HealthWorks clinic (Faraday &amp; El Camino Real, Carlsbad) was obtained and collected in 4-15 BD Vacutainer CPT 8 ml tubes (VWR Cat. #BD362753). The approximate starting total whole blood volume in the CPT tubes for each donor was recorded using the PBMC assay data sheet. 
     The blood sample was remixed immediately prior to centrifugation by gently inverting tubes 8-10 times. CPT tubes were centrifuged at rt (18-25° C.) in a horizontal (swing-out) rotor for 30 min. at 1500-1800 RCF with brake off (2700 RPM Beckman Allegra 6R). The cells were retrieved from the buffy coat interface (between Ficoll and polymer gel layers); transferred to a sterile 50 ml conical tube and pooled up to 5 CPT tubes/50 ml conical tube/donor. The cells were then washed twice with PBS (Ca ++ , Mg ++  free; GIBCO). The tubes were topped up to 50 ml and mixed by inverting several times. The sample was then centrifuged at 330×g for 15 minutes at rt (1215 RPM in Beckman Allegra 6R) and aspirated as much supernatant as possible without disturbing pellet. The cell pellet was dislodged by gently swirling tube and resuspended cells in RPMI+10% FBS+pen/strep (˜1 ml/10 ml starting whole blood volume). A 60 μl sample was pipette into a sample vial (Beckman Coulter) with 600 μl VersaLyse reagent (Beckman Coulter Cat #A09777) and was gently vortexed for 10-15 sec. The sample was allowed to incubate for 10 min. at rt and being mixed again before counting. The cell suspension was counted on Vicell XR cell viability analyzer (Beckman Coulter) using PBMC cell type (dilution factor of 1:11 was stored with other parameters). The live cell/ml and viability were recorded. The cell suspension was diluted to 1×10 7  live PBMC/ml in RPMI+10% FBS+pen/strep. 
     The cells were plated at 5×10 5  in 50 μl/well of 96-well tissue culture plate (Falcon Microtest). 50 μl/well of 2× concentration oligos/controls diluted in RPMI+10% FBS+pen/strep. was added according to experiment template (100 μl/well total). Plates were placed on the shaker and allowed to mix for approx. 1 min. After being incubated for 24 hrs at 37° C.; 5% CO 2 , the plates were centrifuged at 400×g for 10 minutes before removing the supernatant for MSD cytokine assay (i.e. human IL-6, IL-10, and TNF-α). 
     Compound 353512 is an internal standard known to be a high responder for IL-6 release in the assay, while compound 104838 is a negative control. The hPBMCs were isolated from fresh, volunteered donors and were treated with modified oligonucleotide at 0.064, 0.32, and 1.6 200 μM concentrations. After a 24 hr treatment, the cytokine levels were measured and averaged across two donors. The results presented in the table below show that selected modified oligonucleotides targeting human α-ENaC have low proinflammatory responses in human peripheral mononuclear blood cells. 
     
       
         
           
               
             
               
                 TABLE 26 
               
             
            
               
                   
               
               
                 Modified oligonucleotides 
               
               
                 tested as controls in hPBMC assay 
               
            
           
           
               
               
               
               
            
               
                 Compound 
                 Sequence  
                   
                 SEQ 
               
               
                 No. 
                 (5′to 3′) 
                 Target 
                 ID No. 
               
               
                   
               
               
                 104838 
                 G es   m C es T es G es A es   
                 TNFα 
                 1955 
               
               
                   
                 T ds T ds A ds G ds A ds   
                   
                   
               
               
                   
                 G ds A ds G ds A ds G ds   
                   
                   
               
               
                   
                 G es T es   m C es   m C es   
                   
                   
               
               
                   
                   m C e   
                   
                   
               
               
                   
               
               
                 353512 
                 T es   m C es   m C es   m C ds   
                 CRP 
                 1956 
               
               
                   
                 A ds T ds T ds T ds   
                   
                   
               
               
                   
                   m C ds A ds G ds   
                   
                   
               
               
                   
                 G ds A ds G ds A ds   m C ds   
                   
                   
               
               
                   
                   m C ds T es G es G e   
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 27 
               
             
            
               
                   
               
               
                 Results of hPBMC Assay for Selected Modified Oligonucleotides 
               
            
           
           
               
               
               
               
               
            
               
                 Compound 
                   
                 IL-10 
                 IL-6 
                 TNF-α 
               
               
                 No. 
                 Dose 
                 (pg/mL) 
                 (pg/mL) 
                 (pg/mL) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 104838 
                 0.064 
                 7.4 
                 63.4 
                 10.5 
               
               
                 (−control) 
                 0.32 
                 8.9 
                 75.2 
                 11.6 
               
               
                   
                 1.6 
                 12.7 
                 118.9 
                 19.2 
               
               
                 353512 
                 0.064 
                 26.7 
                 130.0 
                 14.6 
               
               
                 (+control) 
                 0.32 
                 39.9 
                 199.9 
                 17.0 
               
               
                   
                 1.6 
                 33.0 
                 230.4 
                 27.7 
               
               
                 797236 
                 0.064 
                 9.4 
                 59.2 
                 10.4 
               
               
                   
                 0.32 
                 20.1 
                 105.5 
                 13.4 
               
               
                   
                 1.6 
                 27.3 
                 173.1 
                 19.5 
               
               
                 797308 
                 0.064 
                 5.6 
                 55.9 
                 9.3 
               
               
                   
                 0.32 
                 7.6 
                 60.7 
                 10.8 
               
               
                   
                 1.6 
                 9.5 
                 83.6 
                 13.4 
               
               
                 797313 
                 0.064 
                 4.6 
                 56.0 
                 8.8 
               
               
                   
                 0.32 
                 8.9 
                 55.5 
                 10.7 
               
               
                   
                 1.6 
                 14.2 
                 95.8 
                 14.7 
               
               
                 797468 
                 0.064 
                 7.1 
                 94.0 
                 9.9 
               
               
                   
                 0.32 
                 6.7 
                 53.4 
                 9.7 
               
               
                   
                 1.6 
                 11.8 
                 103.5 
                 15.0 
               
               
                 797495 
                 0.064 
                 5.5 
                 63.1 
                 9.6 
               
               
                   
                 0.32 
                 8.5 
                 58.9 
                 10.8 
               
               
                   
                 1.6 
                 8.9 
                 83.1 
                 15.2 
               
               
                 826262 
                 0.064 
                 6.1 
                 50.8 
                 9.7 
               
               
                   
                 0.32 
                 13.0 
                 81.5 
                 12.2 
               
               
                   
                 1.6 
                 10.4 
                 98.2 
                 14.2 
               
               
                 826632 
                 0.064 
                 4.1 
                 55.0 
                 9.4 
               
               
                   
                 0.32 
                 6.6 
                 65.8 
                 10.8 
               
               
                   
                 1.6 
                 7.5 
                 111.3 
                 15.5 
               
               
                 826743 
                 0.064 
                 4.4 
                 60.1 
                 9.2 
               
               
                   
                 0.32 
                 7.7 
                 63.8 
                 11.1 
               
               
                   
                 1.6 
                 6.0 
                 81.8 
                 16.1 
               
               
                 826763 
                 0.064 
                 4.5 
                 58.2 
                 9.6 
               
               
                   
                 0.32 
                 8.9 
                 63.1 
                 10.8 
               
               
                   
                 1.6 
                 11.6 
                 116.7 
                 20.9 
               
               
                 826819 
                 0.064 
                 4.7 
                 51.6 
                 8.2 
               
               
                   
                 0.32 
                 4.3 
                 52.5 
                 7.9 
               
               
                   
                 1.6 
                 7.3 
                 62.8 
                 11.3 
               
               
                 826906 
                 0.064 
                 4.4 
                 48.3 
                 7.6 
               
               
                   
                 0.32 
                 4.8 
                 68.9 
                 9.2 
               
               
                   
                 1.6 
                 6.3 
                 60.4 
                 13.9 
               
               
                 827030 
                 0.064 
                 3.7 
                 40.8 
                 7.9 
               
               
                   
                 0.32 
                 5.4 
                 42.4 
                 7.5 
               
               
                   
                 1.6 
                 4.5 
                 54.1 
                 8.4 
               
               
                 827200 
                 0.064 
                 4.2 
                 49.4 
                 8.9 
               
               
                   
                 0.32 
                 5.3 
                 67.6 
                 9.5 
               
               
                   
                 1.6 
                 5.4 
                 55.2 
                 9.5 
               
               
                 827288 
                 0.064 
                 4.5 
                 44.1 
                 7.7 
               
               
                   
                 0.32 
                 6.0 
                 50.2 
                 8.7 
               
               
                   
                 1.6 
                 7.6 
                 76.3 
                 14.9 
               
               
                 827307 
                 0.064 
                 4.6 
                 62.2 
                 9.9 
               
               
                   
                 0.32 
                 5.3 
                 52.3 
                 9.0 
               
               
                   
                 1.6 
                 5.0 
                 54.1 
                 10.6 
               
               
                 827347 
                 0.064 
                 8.3 
                 53.6 
                 10.9 
               
               
                   
                 0.32 
                 20.7 
                 115.2 
                 12.8 
               
               
                   
                 1.6 
                 33.9 
                 163.3 
                 21.1 
               
               
                 827359 
                 0.064 
                 5.8 
                 61.8 
                 9.4 
               
               
                   
                 0.32 
                 6.2 
                 52.7 
                 10.3 
               
               
                   
                 1.6 
                 11.0 
                 75.2 
                 11.8 
               
               
                 827372 
                 0.064 
                 4.7 
                 56.5 
                 8.8 
               
               
                   
                 0.32 
                 7.3 
                 65.4 
                 9.6 
               
               
                   
                 1.6 
                 13.1 
                 81.3 
                 13.1 
               
               
                 827392 
                 0.064 
                 4.5 
                 45.5 
                 7.7 
               
               
                   
                 0.32 
                 5.1 
                 48.0 
                 8.8 
               
               
                   
                 1.6 
                 5.4 
                 50.9 
                 9.9 
               
               
                 827414 
                 0.064 
                 5.5 
                 51.6 
                 8.7 
               
               
                   
                 0.32 
                 7.6 
                 58.1 
                 10.3 
               
               
                   
                 1.6 
                 16.6 
                 102.4 
                 16.3 
               
               
                 827419 
                 0.064 
                 4.2 
                 52.5 
                 7.9 
               
               
                   
                 0.32 
                 7.5 
                 62.0 
                 11.2 
               
               
                   
                 1.6 
                 8.0 
                 93.8 
                 16.5 
               
               
                 827497 
                 0.064 
                 4.5 
                 50.5 
                 8.3 
               
               
                   
                 0.32 
                 5.1 
                 56.9 
                 9.5 
               
               
                   
                 1.6 
                 5.8 
                 73.7 
                 13.0 
               
               
                   
               
            
           
         
       
     
     Example 9: Effects of a Modified Oligonucleotide Complementary to α-ENaC in a Mouse Model of Cystic Fibrosis 
     A modified oligonucleotide complementary to mouse α-ENaC was tested for its effects on preventing and treating airway restriction in a mouse model of cystic fibrosis. Treatment of wild type mice with a modified oligonucleotide complementary to Nedd4L induced a cystic fibrosis-like phenotype (See Crosby et al.  J. of Cystic Fibrosis,  2017). Compound 668395 has a 3-10-3 phosphothiorate cEt gapmer motif. It is 16 nucleobases in length, wherein the central gap segment contains ten 2′-deoxynucleosides and is flanked by wing segments on the 3′ and 5′ ends, each containing three cEt nucleosides. All cytosine residues throughout the modified oligonucletoide are 5-methyl cytosines. The internucleoside linkages are all phosphorothioate internucleoside linkages. The sequence is GAGCATCTAATACAGC (SEQ ID NO: 1958), which is 100% complementary to mouse α-ENaC. 
     Adult mice were treated twice a week for 2 weeks with compound 668395 or vehicle (control) at 0.33 mg/kg/dose via aerosol dosing. Then, mice were treated with an antisense oligonucleotide that reduces Nedd4L (Nedd 4L ASO) via oropharyngeal dosing at 10 mg/kg/dose once a week for 6 weeks. After 8 weeks, airway restriction was tested with a methacholine challenge. Lung function was measured using the Penh score obtained through unrestrained plethysmography. A higher Penh score indicates more lung constriction. Each group contained 8 mice. The results, shown in the table below, indicate that pre-treatment with a modified oligonucleotide complementary to α-ENaC prevented the decrease in lung function observed in the cycstic fibrosis mouse model. 
     
       
         
           
               
             
               
                 TABLE 28 
               
             
            
               
                   
               
               
                 Penh scores 
               
            
           
           
               
               
            
               
                   
                 Methacholine (mg/mL) 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 0 
                 3 
                 6 
                 12 
                 25 
               
            
           
           
               
               
               
            
               
                   
                 Treatment 
                 Penh score 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Naïve (saline) 
                 0.7 
                 0.9 
                 1.1 
                 1.3 
                 1.7 
               
               
                   
                 Vehicle + 
                 1.2 
                 1.7 
                 2.1 
                 3.7 
                 5.4 
               
               
                   
                 Nedd4L ASO 
               
               
                   
                 Compound No. 
                 0.9 
                 0.8 
                 1.0 
                 1.2 
                 2.1 
               
               
                   
                 668395 + Nedd4L ASO 
               
               
                   
                   
               
            
           
         
       
     
     In order to test the effect of a modified oligonucleotide complementary to mouse α-ENaC on reversal of airway restriction in a mouse model of cystic fibrosis, adult mice were treated with Nedd4L ASO via oropharyngeal dosing at 10 mg/kg/dose once a week for a total of 9 weeks; and compound 668395 was not administered until week 6. Starting at 6 weeks, mice were administered compound 668395, vehicle, or a control 3-10-3 cEt modified oligonucleotide (control compound) via aerosol dosing three times per week for three weeks. Lung function was tested with a methacholine challenge prior to the first treatment at 6 weeks and at 9 weeks, and Penh scores were obtained through unrestrained plethysmography. Each group contained 12 mice. The results, shown in the tables below, indicate that treatment with a modified oligonucleotide complementary to α-ENaC restored lung function in a mouse model of cystic fibrosis. 
     
       
         
           
               
             
               
                 TABLE 29 
               
             
            
               
                   
               
               
                 Penh scores at 6 weeks 
               
            
           
           
               
               
            
               
                   
                 Methacholine (mg/mL) 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 0 
                 3 
                 6 
                 12 
                 25 
               
            
           
           
               
               
               
            
               
                   
                 Treatment 
                 Penh score 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Naïve (no treatment) 
                 0.7 
                 0.8 
                 0.9 
                 1.3 
                 2.4 
               
               
                   
                 Nedd4L ASO 
                 0.9 
                 1.3 
                 1.7 
                 3.1 
                 4.5 
               
               
                   
                 (baseline scores for 
               
               
                   
                 vehicle group) 
               
               
                   
                 Nedd4L ASO 
                 0.8 
                 1.2 
                 1.6 
                 2.5 
                 4.7 
               
               
                   
                 (baseline scores for 
               
               
                   
                 compound 668395 group) 
               
               
                   
                 Nedd4L ASO 
                 1.0 
                 1.3 
                 1.8 
                 2.9 
                 5.1 
               
               
                   
                 (baseline scores for 
               
               
                   
                 control compound group) 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 30 
               
             
            
               
                   
               
               
                 Penh scores at 9 weeks 
               
            
           
           
               
               
            
               
                   
                 Methacholine (mg/mL) 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 0 
                 3 
                 6 
                 12 
                 25 
               
            
           
           
               
               
               
            
               
                   
                 Treatment 
                 Penh score 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Naïve (saline) 
                 0.7 
                 0.9 
                 0.9 
                 1.0 
                 1.8 
               
               
                   
                 Nedd4L ASO + 
                 1.1 
                 1.2 
                 1.5 
                 2.3 
                 4.0 
               
               
                   
                 vehicle 
               
               
                   
                 Nedd4L ASO + 
                 0.9 
                 1.0 
                 1.1 
                 1.3 
                 1.9 
               
               
                   
                 compound 668395 
               
               
                   
                 Nedd4L ASO + 
                 1.1 
                 1.1 
                 1.6 
                 2.4 
                 4.2 
               
               
                   
                 control compound 
               
               
                   
                   
               
            
           
         
       
     
     Example 10: Effect of Modified Oligonucleotides Complementary to Human α-ENaC on Cystic Fibrosis Patient Derived Primary Human Bronchial Epithelial Cells 
     Primary human bronchial epithelial cells from cystic fibrosis patients were obtained from Epithelix. Cells were cultured at an Air-Liquid Interface (ALI) on transwell membrane inserts (Corning®) with PneumaCult™-ALI Medium (StemCell Technologies) on the basolateral side of the membrane. At 6 weeks post seeding, cells were treated either with ION No. 827359, or with ION No. 549148 (3-10-3 cET gapmer, GGCTACTACGCCGTCA, designated herein as SEQ ID NO: 1959), that served as a negative control that does not target α-ENaC. Both modified oligonucleotides were treated using free uptake at a concentration of 104 on the basolateral side. Cells were lysed 72 hours post treatment. 
     Human α-ENaC Expression Levels 
     Total RNA was isolated from the cells 72 hours post treatment. α-ENaC mRNA levels were measured using human primer probe set hSCNN1A_LTS01170. α-ENaC mRNA levels were normalized to cyclophilin A. Cyclophilin A was amplified using primer-probe set HTS3936 (forward sequence, GCCATGGAGCGCTTTGG, designated herein as SEQ ID NO: 1960; reverse sequence, TCCACAGTCAGCAATGGTGATC, designated herein as SEQ ID NO: 1961; probe sequence, TCCAGGAATGGCAAGACCAGCAAGA, designated herein as SEQ ID NO: 1962). Results are presented in the tables below as percent control of the amount of α-ENaC mRNA relative to control cells (% control). 
     
       
         
           
               
             
               
                 TABLE 31 
               
             
            
               
                   
               
               
                 Inhibition of α-ENaC mRNA by in cystic fibrosis patient 
               
               
                 derived primary human bronchial epithelial cells 
               
            
           
           
               
               
               
            
               
                   
                 ION No. 
                 % control 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 549148 
                 100 
               
               
                   
                 827359 
                 7 
               
               
                   
                   
               
            
           
         
       
     
     Measurement of Amiloride Sensitive Current 
     72 hours post treatment with modified oligonucleotide, the transwell inserts were mounted in Using chambers (Physiologic Instruments, San Diego, Calif.). Short-circuit current (I sc ) was measured. Data were analyzed using ACQUIRE &amp; ANALYZE 2.3 (Physiologic Instruments). The basolateral solution contained (in mM) 145 NaCl, 3.3 K2HPO4, 0.8 KH2PO4, 1.2 MgCl2, 1.2 CaCl2, 10 glucose, 10 Hepes (adjusted to pH 7.35 with NaOH) and the apical solution contained (in mM) 145 sodium gluconate, 3.3 K2HPO4, 0.8 KH2PO4, 1.2 MgCl2, 1.2 CaCl2, 10 glucose, 10 Hepes (adjusted to pH 7.35 with NaOH)Amiloride was added to apical side at 100 μM. Amiloride-sensitive currents were measured in order to assess ENaC functional activity. 
     
       
         
           
               
             
               
                 TABLE 32 
               
             
            
               
                   
               
               
                 Amiloride response in cystic fibrosis patient derived 
               
               
                 primary human bronchial epithelial cells 
               
            
           
           
               
               
               
            
               
                   
                 ION No. 
                 ΔIsc (μA/cm 2 ) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 549148 
                 −26 
               
               
                   
                 827359 
                 −9 
               
               
                   
                   
               
            
           
         
       
     
     Measurement of Airway Surface Liquid (ASL) 
     72 hours post start of treatment, the effect of modified oligonucleotide on Airway Surface Liquid (ASL) was measured. Immediately before measuring the ASL, cultures were washed three times with PBS to remove excess mucus. 150 μL of KBR buffer (89 mM NaCl, 4 mM KCl, 1.2 mM MgCl2, 1.2 mM CaCl2, 1 mM Hepes, 16 mM Na-gluconate, 10 mM glucose) was added to the apical surface of the cells as the absorption volume. ASL volume was then measured 24 hours, 48 hours and 72 hours post additional of KBR buffer. 
     
       
         
           
               
             
               
                 TABLE 33 
               
             
            
               
                   
               
               
                 ASL volume in cystic fibrosis patient derived 
               
               
                 primary human bronchial epithelial cells 
               
            
           
           
               
               
               
               
            
               
                   
                 Time 
                 ASL volume (μL) 
                   
               
            
           
           
               
               
               
               
            
               
                   
                 (hr) 
                 549148 
                 827359 
               
               
                   
                   
               
            
           
           
               
               
               
               
            
               
                   
                 0 
                 150 
                 150 
               
               
                   
                 24 hr 
                 62 
                 84 
               
               
                   
                 48 hr 
                 20 
                 67 
               
               
                   
                 72 hr 
                 18 
                 38 
               
               
                   
                   
               
            
           
         
       
     
     Example 11: Effect of Combination Treatment of Modified Oligonucleotides with VX-661 (Tezacaftor) and VX-770 (Ivacaftor) 
     Primary human bronchial epithelial cells from cystic fibrosis patients were obtained from Epithelix. Cells were cultured at an Air-Liquid Interface (ALI) on transwell membrane inserts (Corning®) with PneumaCult™-ALI Medium (Stemcell Technologies) on the basolateral side of the membrane for 6 weeks before treatment. At Day 0, Day 4 and Day 8 of treatment, cells were treated either with ION Nos. 827359, or 549148 at 10 μM on the basolateral side of the membrane (a total of 3 doses of each ASO). One set of cells was left untreated with modified oligonucleotide. At Day 11, VX-661 (Tezacaftor) (Medchem Express) was added at 18 μM to both the previously untreated well and to one of the wells treated with ION No. 827359. On Day 14, VX-770 (Ivacaftor) (Medchem Express) was added at 10 μM to the cells previously treated with VX-661. On the same day (Day 14), cultures were washed three times on the apical side with PBS to remove excess mucus. 150 μL of PBS (absorption volume) was added to the apical surface of the cells. ASL volume was measured the next day (Day 15). Combination treatment was found to further increase ASL volume compared to control. 
     
       
         
           
               
             
               
                 TABLE 34 
               
             
            
               
                   
               
               
                 ASL volume in cystic fibrosis patient derived 
               
               
                 primary human bronchial epithelial cells 
               
            
           
           
               
               
               
            
               
                   
                 Treatment 
                 ASL volume (μL) 
               
               
                   
                   
               
               
                   
                 549148 
                 23 
               
               
                   
                 Vx-661 + Vx-770 
                 38 
               
               
                   
                 827359 
                 59 
               
               
                   
                 Vx-661 + Vx-770 + 827359 
                 66