Patent Publication Number: US-2020288742-A1

Title: Natural cocoa alternative and methods of producing same

Description:
CROSS-REFERENCE TO RELATED APPLICATION 
     This application is being filed on Mar. 13, 2018 as a PCT International Patent application and claims priority U.S. patent application Ser. No. 15/699,817, filed on Sep. 8, 2017, the disclosure of which is hereby incorporated by reference in its entirety. This application also claims priority to PCT International Patent Application No. PCT/US2017/050786, filed on Sep. 8, 2017, the disclosure of which is hereby incorporated by reference in its entirety. U.S. patent application Ser. No. 15/699,817 and PCT International Patent Application No. PCT/US2017/050786 each claim the benefit of U.S. Provisional Patent Application No. 62/385,953 filed on Sep. 9, 2016, the disclosure of which is hereby incorporated by reference in its entirety. The U.S. designation claims priority to all of the above-noted applications. To the extent appropriate, a claim of priority is made to each of the above-noted applications. 
    
    
     TECHNICAL FIELD 
     The present disclosure is directed towards a cocoa replacer/alternative/substitute or cocoa enhancer that can be used in a wide range of food products. 
     BACKGROUND 
     Cocoa is a high demand, relatively expensive ingredient used in a wide range of food products. 
     The benefits of cocoa and products made from cocoa are numerous. For example, studies suggest that dark chocolate can reduce the risk of cardiovascular disease. Moreover, unsweetened cocoa is relatively low in calories, and contains essential minerals that support heart, bone, and immune system health. 
     The taste of cocoa is also cherished worldwide and is the basis for drinks and confectionary products of all levels of quality and variety of taste profiles. 
     Cocoa is derived from the beans of the cacao plant. Typically, the beans are cold pressed and the fats (“butter”) pressed out of the beans, yielding powdered cacao, which still contains “living” enzymes from the beans. Cocoa is the roasted (usually at high heat) cacao powder, which deactivates the enzymes. The butter is than added back into the cocoa to produce chocolate (usually with the addition of sugar and other ingredients to enhance flavor and mouthfeel (sensory attributes)). There are a wide variety of cocoa flavors, depending on, e.g., the type of cacao beans and the region they are grown, the amount of sugar present, the amount of free amino acids present, and the cocoa color pigments. 
     There is also a variety of synthetic chocolate flavors that can contain many different synthetic (organic) compounds. Some examples of materials that can be blended to create the artificial tastes and smells of cocoa include butyraldehyde, cocoa hexenal, cocoa pentenal, cocoa resinoid, dimethyl pyrazine, butyl cinnamate, butyl-3,5-(and 3,6)-dimethyl pyrazine and other compounds. 
     In addition, cocoa pricing can be volatile and the industry can experience tight supplies as emerging markets are increasing their consumption of chocolate and cocoa-based products. 
     The food industry would welcome a way to both reduce and/or replace the usage of cocoa or chocolate in a range of food products with a natural (i.e., non-synthetic) alternative to cocoa. 
     SUMMARY 
     In general terms, the present disclosure is directed to an all-natural cocoa substitute made from green banana flour or plantain flour, and methods of producing the same. In a given food product that ordinarily contains cocoa, a cocoa substitute of the present disclosure can be used to replace at least a substantial portion and/or enhance the cocoa. In a given food product that ordinarily contains cocoa, a cocoa substitute of the present disclosure can replace at least a substantial portion of the cocoa in the product. 
     According to certain aspects of the present disclosure, a cocoa substitute is prepared by dry heating (e.g., roasting) green banana flour and/or plantain flour at a predefined temperature for a predefined time. The roasted flour can then be added to a food product to replace and/or enhance cocoa. 
     According to certain aspects of the present disclosure, an edible composition is provided comprising cocoa powder and roasted flour made from green banana and/or plantain, the cocoa powder and the roasted flour having a combined total mass, the roasted flour having a mass from 5% to 90% of the combined total mass. 
     According to certain aspects of the present disclosure, a cocoa replacer and/or enhancer produced by a method of the present disclosure substantially shares at least one and up to six or more sensory attributes in common with cocoa. In some examples, a food product made with a cocoa replacer and/or enhancer produced by a method of the present disclosure substantially shares at least one and up to six or more sensory attributes in common with a reference food product containing the same formulation as the food product, except wherein in the reference food product cocoa is substituted for the cocoa replacer and/or enhancer. Such sensory attributes can include, for examples, a chocolate odor/aroma, a chocolate flavor, chocolate viscosity, bitterness, a toasted odor/aroma, a toasted flavor, a brown color resembling cocoa or chocolate, sweetness, and/or a melting quality. 
     According to further aspects of the present disclosure, a chocolate-flavored food product comprising a flour made from approximately equal parts by mass of roasted green banana flour and cocoa powder is provided. 
     According to further aspects of the present disclosure, a chocolate-flavored food product comprising roasted green banana flour and cocoa powder is provided, wherein the ratio, by mass, of the roasted green banana flour to the cocoa powder, is approximately 1:1 or 1:2 or 1:3 or 1:4 or 1:5 or 1:6 or 1:7 or 1:8 or 1:9 or 1:10, or any ratio in a range from approximately 1:20 to approximately 2:1. 
     According to further aspects of the present disclosure, a method of preparing a chocolate-flavored food product comprises combining cocoa powder and roasted green banana flour, wherein the ratio, by mass, of the roasted green banana flour to the cocoa powder is approximately 1:1 or 1:2 or 1:3 or 1:4 or 1:5 or 1:6 or 1:7 or 1:8 or 1:9 or 1:10, or any ratio in a range from approximately 1:20 to approximately 2:1. 
     In accordance with certain examples of the cocoa alternatives of the present disclosure, the bananas used to make the cocoa alternative/chocolate-flavored product are in Stage 1 or Stage 2 of ripeness. In other examples, the bananas used contain less than 1% sugar content. 
     As mentioned, cocoa and chocolate have a known sensory profile that gives the products certain attributes that identify the products as cocoa or chocolate. Thus, in accordance with certain aspects of the present disclosure, a cocoa replacer and/or enhancer has a sensory profile that is within a range of cocoa or chocolate sensory profiles, and/or within a predetermined deviation from a cocoa or chocolate sensory profile, e.g., within a range of +/−50%, +/−30%, or +/−10% of a cocoa or chocolate sensory profile. 
     In addition, food products made with the roasted green banana flour and/or plantain flour in accordance with the present disclosure can share texture and/or color and/or aroma profiles, at least within a predetermined deviation, with reference products having the same or similar formulation except wherein cocoa is substituted for the roasted green banana/plantain flour. 
     Thus, it has been discovered that roasted green banana flour and roasted plantain flour can provide an economical alternative to cocoa or chocolate with similar sensory attributes. When roasting green banana flour/plantain flour in specific conditions, it has been discovered that the material can develop a cocoa-like flavor, aroma, and color. Thus, in accordance with the present disclosure, roasted green banana flour/plantain flour has the possibility to at least partially replace cocoa or chocolate in many food applications. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWING 
       The FIGURE is a flow chart showing an example method of preparing roasted green banana flour in accordance with the present disclosure. 
     
    
    
     DETAILED DESCRIPTION 
     Seven stages have been described in the ripening of the banana. Stage 1 is when the banana is completely green and has no signs of yellow on the skin. Stage 2 starts to show some slight signs of yellow usually at the distal end (the end farthest from the banana&#39;s point of attachment to the tree) of the banana. At the next three stages, the yellow continues from the distal end towards the proximal end of the fruit. At stage 6 of the ripening the banana is completely yellow. Stage 7 is when the banana starts to develop brown spots and finally turns completely brown. 
     As a banana ripens, the starch in its pulp converts to sugar. Thus, the starch present in the banana is being converted to sugars and the flavor changes from an earthy mild taste to the banana (isoamyl acetate) taste one normally associates with ripe bananas. During the ripening process, the granular starch found in green bananas is being converted to soluble starches and further to sugars. 
     Thus, for a given banana, each ripening stage can be correlated with its sugar content, which is defined herein as the percentage mass of sugars (sucrose, glucose, fructose) of the total mass of that banana&#39;s pulp. For purposes of this disclosure: a Stage 1 banana has a sugar content of 2% or less; a Stage 2 banana has a sugar content greater than 2% up to and including 5%; a Stage 3 banana has a sugar content greater than 5% up to and including 10%; a Stage 4 banana has a sugar content greater than 10% up to and including 25%; a Stage 5 banana has a sugar content greater than 25% up to and including 45%; a Stage 6 banana has a sugar content greater than 45% up to and including 65%; and a Stage 7 banana has a sugar content greater than 65%. 
     As used herein, and unless otherwise noted, the term “green banana” encompasses bananas that are in any of ripeness Stages 1, 2, 3, or 4 as defined herein. The term “green banana” further encompasses plantains having sugar content corresponding to that of any of ripeness Stages 1, 2, 3, and 4 defined herein, as plantains are very similar in composition of starch and proteins to green bananas. 
     As used herein, the term “slurry” refers to the material from peeled mashed green bananas. 
     Green banana flour is the resultant product of drying green banana slurry. Generally, green banana flour is generated by drying green banana slurry at a relatively low temperature so as to minimize disruption of the structure of the starch and protein native to the green banana. 
     Native green banana flour is a type of green banana flour in which the starch is in the native granular form. That is, the starch has not been physically or chemically modified. 
     Already gelatinized (or pre-gelatinized) green banana flour is another type of green banana flour in which the native granular structure of the starch has been partially or completely destroyed in the flour. Some example methods for preparing a pre-gelatinized green banana flour include, but are not limited to, drum drying, spray drying or cooking native green banana flour, followed by precipitation, sonification, or the like. 
     As used herein, the term “green banana flour” encompasses both native green banana flour and pre-gelatinized green banana flour, unless otherwise noted. 
     By processing green banana flour with the application of direct and/or indirect heat (e.g., heat from radiant, microwave, solar, and/or convection sources), it has been found that the existing chemical compounds found within the green banana flour can be changed. 
     Under certain conditions, roasting (i.e., dry heating via one or more direct and/or indirect heating sources) green banana flour changes the flavor and/or aroma notes of the flour such that sensory attributes of chocolate or cocoa can be recognized. This roasted green banana flour, acting as a cocoa/chocolate alternative, can be used in a range of food applications. Examples of such food applications include, but are not limited to, baked goods, pancake mix, cookie mix, smoothies, puddings, dry seasonings, fruit fillings, cereal bars, baby-food, confectionary additive, energy bars, nutritional bars, and many other foods that ordinarily contain cocoa. 
     In at least some examples of the cocoa alternatives of the present disclosure, roasting of pre-gelatinized green banana flour can result in a cocoa alternative that more closely resembles real cocoa than does roasting of native green banana flour. Without being bound by any particular theory, it is believed that the starch chains of pre-gelatinized green banana flour are more readily rearranged by the roasting process into the compounds that adopt cocoa-like attributes than are the starch chains of native green banana flour. For example, the roasting process produces complex sugars, which are at least partially responsible for the sensory (taste, smell and texture) attributes associated with such roasted products. In some examples, such sugars may be more readily achieved from roasting pre-gelatinized green banana flour than native green banana flour. 
     Cocoa is a complex material with a variety of sensory attributes (flavors and smells) depending on where it was grown and how it was processed (e.g., roasted). Some examples of taste attributes associated with cocoa may include, e.g.: alkali, aromatic, astringent, bitter, chocolate, cocoa, fruity, roasted, sour, baggy, burnt and earthy. Some examples of aroma attributes associated with cocoa may include, e.g., pungent, cocoa, musty, malty, bready, sweet, chocolate, vanilla and bitter. 
     It has been discovered that a variety of these attributes can be obtained from the roasted green banana flour (either native or pre-gelatinized) of the present disclosure. 
     Thus, according to some examples, roasted green banana flour in accordance with the present disclosure will have at least one of the sensory attributes associated with cocoa. According to other examples, roasted green banana flour in accordance with the present disclosure will have at least two of the sensory attributes associated with cocoa. According to other examples, roasted green banana flour in accordance with the present disclosure will have at least three of the sensory attributes associated with cocoa. According to other examples, roasted green banana flour in accordance with the present disclosure will have four or more of the sensory attributes associated with cocoa. 
     In some examples, roasted green banana flour of the present disclosure includes only roasted native green banana flour. In some examples, roasted green banana flour of the present disclosure includes only roasted pre-gelatinized green banana flour. In some examples, roasted green banana flour of the present disclosure includes both roasted pre-gelatinized green banana flour and roasted native green banana flour. 
     In some examples, roasted green banana flour of the present disclosure includes green banana flour made from one or more of: plantains, green bananas in Stage 1 of ripeness, green bananas in Stage 2 of ripeness, green bananas in Stage 3 of ripeness, and/or green bananas in Stage 4 of ripeness. In particular examples, roasted green banana flour of the present disclosure includes green banana made only from one or more of: plantains, green bananas in Stage 1 of ripeness, and/or green bananas in Stage 2 of ripeness. 
     Referring now to the FIGURE, in an example process  100  of preparing roasted green banana flour in accordance with the present disclosure, in an optional step  102  green banana flour is evenly distributed on a substantially flat surface, such as a tray, such that grouping or clumping of the green banana flour are minimized, and such that a substantially uniform layer of the green banana flour is formed on the surface. In some examples, a non-stick material, such as a parchment paper, can line the flat surface to minimize sticking of the green banana flour to the surface. 
     In a subsequent step  104  the green banana flour is roasted for a predefined time at a predefined temperature. 
     In an optional subsequent step  106 , the green banana flour is redistributed on the flat surface such that grouping and clumping of the green banana flour are minimized, and such that a substantially uniform layer of the green banana flour is formed on the surface. 
     In an optional subsequent step  108 , the green banana flour is roasted for a predefined time. 
     In an optional subsequent step  110 , the steps  106  and  108  are repeated in sequence one, two, three, or more times, although the predefined time and/or temperature for each roasting may vary. 
     In an optional subsequent step  112 , the roasted green banana flour is cooled, e.g., by placing a tray holding the roasted green banana flour on a wire rack. 
     In an optional step  114 , the cooled (or not cooled) roasted green banana flour is packaged, e.g., in a hermetically sealed package or added to a food product as a cocoa alternative. 
     In an optional step  116 , the roasted green banana flour is added to a food product as a cocoa alternative. 
     The roasting step(s) of the process  100  can be facilitated by any appropriate heat source, e.g., a convection oven. The roasting steps can include one or more of air roasting, fluidized bed roasting, drum roasting, oven roasting, rotary tray roasting and/or coffee roasting. 
     In some examples, the predefined temperature of each of one or more of the roasting(s) in the process  100  is in a range from 160° C. to 220° C. In some examples, the predefined temperature of one or more of the roasting(s) in the process  100  is in a range from 170° C. to 205° C. In some examples, the predefined temperature of one or more of the roasting(s) in the process  100  is in a range from 190° C. to 205° C. In some examples, the predefined temperature of one or more of the roasting(s) in the process  100  is in a range from 176° C. to 177° C. 
     Variations in the example temperatures and times can vary from those described herein, such as variations of up to +/−10 percent, or up to +/−20 percent. Additionally, the times and temperatures can be different depending on the volume of green banana flour being processed. 
     It should also be appreciated that the use of different roasting temperatures and/or combinations of roasting temperatures can result in different cocoa-like sensory attribute profiles of the final roasted green banana flour. In at least some examples, the roasting temperature(s) is/are selected to minimize burnt flavors or aromas in the final roasted green banana flour. 
     In some examples, the predefined heating time of each of one or more of the roasting(s) in the process  100  is in a range from 10 minutes to 60 minutes. In some examples, the predefined heating time of each of one or more of the roasting(s) in the process  100  is in a range from 15 minutes to 45 minutes. In some examples, the predefined heating time of each of one or more of the roasting(s) in the process  100  is in a range from 15 minutes to 30 minutes. In some examples, the total combined roasting time from all the roasting steps of the process  100  is in a range from 10 minutes to 120 minutes. In some examples, the total combined roasting time from all the roasting steps of the process  100  is in a range from 10 minutes to 60 minutes. In some examples, the total combined roasting time from all the roasting steps of the process  100  is in a range from 15 minutes to 45 minutes. In some examples, the total combined roasting time from all the roasting steps of the process  100  is in a range from 15 minutes to 30 minutes. In some examples, the process  100  includes two, three, or four of the roasting steps, each of the roasting steps being in a range from 176° C. to 177° C. for 15 minutes or approximately 15 minutes. 
     In some examples, between roasting steps of the process  100 , the green banana flour is allowed to cool by a predefined amount. 
     The process  100  can include one, two, three, four, five, six or more of the roasting steps, optionally with a redistribution of the green banana flour and/or cooling of the green banana flour between some or all of the roasting steps. 
     The roasted green banana flour produced by the process  100  can be used as an all-natural cocoa or chocolate alternative in a variety of foods and can have one or more of the following properties: all-natural, clean label, vegetarian, vegan, kosher, halal, gluten free, conventional or organic, containing fiber material and/or resistant starch, non-GMO, having chocolate flavor and notes but contains no chocolate, and allow for reduction or replacement of real chocolate or cocoa in a range of applications. The roasted green banana flour produced by the process  100  can provide viscosity enhancements, and/or nutritional enhancements to a food product, such as potassium, magnesium and manganese. In addition, the roasted green banana flour produced by the process  100  can be made from 100% fruit. 
     Cocoa and chocolate have known sensory attributes. These attributes are sometimes defined as including, for example, appearance (including color), odor/aroma, flavor, texture, and global quality. In some examples, the roasted green banana flour produced by the process  100  shares at least one, two or more of the sensory attributes of cocoa or chocolate, such as: a chocolate smell, a chocolate flavor, bitterness, a toasted smell, a toasted flavor, a brown color, and/or sweetness. 
     Food Product Examples 
     Described below are three example food products prepared with cocoa and/or roasted green banana flour, as well as experiments conducted to compare each of the example food products as between a control, which contained no roasted green banana flour, and test samples in which some amount of the cocoa was replaced with roasted green banana flour. 
     In the test samples, the green banana flour used for roasting included one or both of NuBana™ N100 (native) green banana flour and NuBana™ P500 (pre-gelatinized) green banana flour (available from International Agriculture Group, 106 LangTree Village Drive, Suite 301, Mooresville, N.C. 28117). The green banana flour was roasted using a conventional oven on baking sheets. The sample weight of green banana flour per sheet was 300 g. The baking sheet was then placed in a pre-heated oven at the predefined temperature and roasted for the predefined time. Roasting temperatures were accomplished at 163° C., 177° C., 190° C., 205° C. and 218° C. and the roasting times varied from 15 to 60 minutes in 5-minute increments. It should be appreciated that, using other roasting techniques, temperature above 218° C. may be used to provide acceptable products and are considered to be within the scope of this disclosure. Once the green banana flour had been roasted for the appropriate amount of time the sheet was removed from the oven and allowed to cool to room temperature by placing the tray on a wire rack. For the food product preparations, the roasted green banana flour was then placed in a sealed container for storage or until testing could be accomplished. Sensory attribute testing of different roasted green banana flour preparations themselves was also conducted and is described further below in the sensory attribute experiments. 
     Food Product Example 1—Preparation of Non-Dairy Chocolate Drink 
     A total of 60 g of cocoa powder was mixed with 20 g of granulated sugar and mixed until uniform. A coffee filter was placed in a 10 cm long stemmed glass funnel and the mixture of roasted cocoa powder and sugar was placed in the coffee filter. A total of 1 L of boiling water (100° C.) was poured through the filter and collected in a 2 L beaker. The liquid product was then placed aside to cool to room temperature (20° C.-25° C.). The liquid product was placed in a sealed glass container until taste testing could be performed. 
     Food Product Example 2—Preparation of a Dairy Chocolate Drink 
     A mixture of 12 g of cocoa powder and 6 g of granulated table sugar were diluted with 200 g of a half cream—half whole milk dairy product at 18° C. The mixture was placed in a Waring® blender and blended on high for 70 seconds. The sample was removed from the blender cup and placed in a sealed container until taste testing was conducted. The beverages were tasted at 21° C. 
     Food Product Example 3—Preparation of a First Chocolate Coating Compound 
     The preparations were prepared using a mixing ball mill (Selmi Micro Ball Mill, available from Selmi, Via Statale, 151-12069 Santa Vittoria d&#39;Alba) using the following procedure. The test sample compositions are shown below in Table 1. 
     Approximately half of the oil and all of the sugar and salt were added to the top of the ball mill. The mixture was milled and recirculated for 3-5 minutes. The temperature of the mill was not permitted to exceed 50° C. Cooling was used as necessary to maintain the temperature below 50° C. The lecithin, milk powder and remaining oil (holding back 2%) was added and milled until the fineness of the product was below 30 microns. The fineness was checked in a mixture of 30% mineral oil with a handheld micrometer. The content of the mill was emptied into a clean mixing bowl and the cocoa powder and roasted green banana flour was added to the mixture and mixed with a standard baking mixer at low to medium speed with a mixing attachment, scraping the sides occasionally. During mixing, heat was added to the bowl by means of a heat gun keeping the mixture between 45° C. and 50° C. The formulation was mixed for 10-15 minutes until completely uniform. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Formulations for a chocolate coating compound 
               
            
           
           
               
               
            
               
                   
                 Test Sample Number 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Ingredient* 
                 Control 
                 1 
                 2 
                 3 
                 4 
                 5 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Sugar 
                 55.45 
                 55.45 
                 55.45 
                 55.45 
                 55.45 
                 55.45 
               
               
                 Palm Kernel Oil 
                 29 
                 29 
                 29 
                 29 
                 29 
                 29 
               
               
                 Whole Milk Powder 
                 3 
                 3 
                 3 
                 3 
                 3 
                 3 
               
               
                 Sunflower Lecithin 
                 0.5 
                 0.5 
                 0.5 
                 0.5 
                 0.5 
                 0.5 
               
               
                 Salt 
                 0.05 
                 0.05 
                 0.05 
                 0.05 
                 0.05 
                 0.05 
               
               
                 Natural Cocoa 
                 12 
                 10.8 
                 10.8 
                 9 
                 6 
                 6 
               
               
                 Powder 
               
               
                 NuBana ™ N100 
                 — 
                 1.2 
                 — 
                 3 
                 6 
                 3 
               
               
                 green banana flour 
               
               
                 roasted 40 minutes 
               
               
                 @ 205° C. 
               
               
                 NuBana ™ P500 
                 — 
                 — 
                 1.2 
                 — 
                 — 
                 — 
               
               
                 green banana flour 
               
               
                 roasted 15 minutes 
               
               
                 @ 205° C. 
               
               
                   
               
               
                 *all values are in parts by mass 
               
            
           
         
       
     
     Food Product Example 4—Preparation of a Chocolate Brownie 
     An oven was pre-heated to 175° C. Muffin tins were sprayed with non-stick cooking spray. The flour, sugar, cocoa powder, and NuBana™ N100 green banana flour (which had been heated at 205° C. for 40 minutes) in the amounts set forth in Table 2 below were added to a mixing bowl. In another small bowl, the butter and eggs were whisked together. The egg mixture and the oil were then added to the mixing bowl and mixed for approximately two minutes until a uniform batter was obtained. The batter was then divided evenly into the muffin tins and baked for 20 minutes. The tins were removed from the oven and allowed to cool for five minutes. The brownies were then removed from the pan and allowed to cool on a wire rack for at least one hour. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Brownie formulations 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                 Test Sample 9 
                 Test Sample 10 
                 Test Sample 11 
               
               
                   
                   
                 10% cocoa 
                 25% cocoa 
                 50% cocoa 
               
               
                   
                 Control 
                 Replacement 
                 Replacement 
                 Replacement 
               
               
                   
               
               
                 All Purpose 
                  57 g 
                  57 g 
                  57 g 
                  57 g 
               
               
                 Flour 
                   
                   
                   
                   
               
               
                 Sugar 
                 210 g 
                 210 g 
                 210 g 
                 210 g 
               
               
                 Vegetable 
                 103 g 
                 103 g 
                 103 g 
                 103 g 
               
               
                 Oil 
                   
                   
                   
                   
               
               
                 Eggs (Large) 
                 2 each 
                 2 each 
                 2 each 
                 2 each 
               
               
                 Salt 
                  1 g 
                  1 g 
                  1 g 
                  1 g 
               
               
                 Cocoa 
                  33 g 
                 29.7 g  
                 24.74 g   
                 16.5 g  
               
               
                 Natural 
                   
                   
                   
                   
               
               
                 Roasted 
                  0.0 g  
                  3.3 g  
                 8.25 g  
                 16.5 g  
               
               
                 NuBana ™ 
                   
                   
                   
                   
               
               
                 N100 Green 
                   
                   
                   
                   
               
               
                 Banana Flour 
               
               
                   
               
            
           
         
       
     
     Food Product Example 5—Preparation of a Second Chocolate Coating Compound 
     Samples of a chocolate coating compound were made by replacing portions of natural cocoa powder in a standard chocolate compound formula (the control). The compound was made using a laboratory chocolate refiner. 
     The compound coating was made using the standard ball mill procedure with the ingredients from Food Product Example 3 above, but without the cocoa or roasted green banana flour. The batch was split and cocoa powder was added at 12% usage for the control and a blend of cocoa powder and roasted NuBana™ N100 green banana flour (roasted at 205° C. for 40 minutes) was used for each of three test samples (Test Samples 12, 13, and 14) as shown in the Table 3 below. The powders were added and mixed in a standard baking mixer. The compound samples were molded at 37° C. in wafer molds and put in a freezer for 5 minutes to set up before demolding. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 chocolate coating formulations (% by mass) 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                 Sample 12 
                 Sample 13 
                 Sample 14 
               
               
                   
                   
                 10% cocoa 
                 25% cocoa 
                 50% Cocoa 
               
               
                 Ingredient 
                 Control 
                 Replacement 
                 Replacement 
                 Replacement 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Sugar 
                 55.45% 
                 55.45% 
                 55.45% 
                 55.45% 
               
               
                 Palm Kernel 
                   29% 
                   29% 
                   29% 
                   29% 
               
               
                 Oil 
                   
                   
                   
                   
               
               
                 Whole Milk 
                    3% 
                    3% 
                    3% 
                    3% 
               
               
                 Powder 
                   
                   
                   
                   
               
               
                 Sunflower 
                   .5% 
                   .5% 
                   .5% 
                   .5% 
               
               
                 Lecithin 
                   
                   
                   
                   
               
               
                 Salt 
                  .05% 
                  .05% 
                  .05% 
                  .05% 
               
               
                 Natural 
                   12% 
                  10.8% 
                    9% 
                    6% 
               
               
                 Cocoa 
                   
                   
                   
                   
               
               
                 Powder 
                   
                   
                   
                   
               
               
                 Roasted 
                    0% 
                  1.2% 
                    3% 
                    6% 
               
               
                 NuBana ™ 
                   
                   
                   
                   
               
               
                 N100 Green 
                   
                   
                   
                   
               
               
                 Banana Flour 
               
               
                   
               
            
           
         
       
     
     Sensory Attribute Experiments 
     To establish a sensory attribute baseline, a panel tested the flavor and aroma attributes associated with cocoa powder. The panel was trained on attribute descriptors using the following definitions and references. 
     Attribute: Acid/Sour 
     Definition: the taste stimulated by acids. 
     Reference: 0.08% citric acid in water. 
     Attribute: Bitter 
     Definition: the taste stimulated by caffeine. 
     Reference: caffeine in spring water (0.1% concentration). 
     Attribute: Cacao: 
     Definition: the characteristic aroma of roasted cocoa beans. 
     Reference: Ivory Coast roasted cocoa nibs (cocoa mass, lab scale). 
     Attribute: Roasted 
     Definition: the aroma associated with food items that have been browned by heat treatment. 
     Attribute: Alkaline 
     Definition: the aroma associated with baking soda. 
     Reference: salt solution (0.7%; 1.4 grams salt to 300 grams water) as compared with a baking soda solution (5% solution, or 15 grams baking soda to 300 g water) 
     Attribute: Hay/Straw 
     Definition: the aroma associated with sweet, sun-dried grasses. 
     Reference: hay, dried parsley. 
     Attribute: Earthy: 
     Definition: the aroma associated with topsoil. 
     Reference: raw skins of russet potatoes. 
     Attribute: Moldy/Jute Bag 
     Definition: the aroma reminiscent of items stored under highly humid conditions (damp basements, molds). 
     Reference: brie cheese. 
     Attribute: Smoky 
     Definition: the aroma associated with wood smoke odor. 
     Reference: liquid smoke. 
     Experiment 1 
     The trained panel was presented with a control preparation containing cocoa powder and a test sample preparation containing NuBana™ N100 green banana flour roasted at 205° C. for the times shown below. All taste tests were conducted at room temperature. The panelists sampled preparations of the cocoa control and the roasted green banana flour prepared according to Food Product Example 1 above, wherein for the test samples NuBana™ N100 green banana flour was substituted for the cocoa powder. 
     Results: 
     Cocoa Control: 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                 Panelist 1 
                 Panelist 2 
                 Panelist 3 
                 Panelist 4 
                 Panelist 5 
                 Panelist 6 
               
               
                   
               
             
            
               
                 astringent 
                 astringent 
                 sour/bitter 
                 sweet 
                 astringent 
                 bitter 
               
               
                 bitter 
                 bitter 
                 Sweet 
                 bitter 
                 sweet 
                 sweet 
               
               
                   
                   
                   
                   
                   
                 roasted 
               
               
                 sweet 
                 sweet 
                 Cocoa 
                 cocoa 
                 sour 
                 sour 
               
               
                 cocoa 
                 low cocoa 
                 tongue 
                   
                 bitter 
                 cocoa 
               
               
                   
                   
                 drying 
                   
                   
                   
               
               
                   
                 sour 
               
               
                   
               
            
           
         
       
     
     NuBana™ N100 green banana flour roasted for 15 minutes: 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                 Panelist 1 
                 Panelist 2 
                 Panelist 3 
                 Panelist 4 
                 Panelist 5 
                 Panelist 6 
               
               
                   
               
             
            
               
                 bitter 
                 bitter 
                 bitter 
                 astringent 
                 bitter 
                 astringent 
               
               
                 sweet 
                 sweet 
                 astringent 
                 sweet 
                 sour 
                 sour 
               
               
                   
                 fruity 
                   
                   
                   
                   
               
               
                 astringent 
                   
                 sweet 
                 sour 
                 sweet 
                   
               
               
                 sour 
                   
                 sour 
                 bitter 
                   
                 fruity 
               
               
                   
                   
                   
                 astringent 
                   
                 tongue 
               
               
                   
                   
                   
                 tongue 
                   
                   
               
               
                   
                   
                   
                 drying 
               
               
                   
               
            
           
         
       
     
     NuBana™ N100 green banana flour roasted for 20 minutes: 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                 Panelist 1 
                 Panelist 2 
                 Panelist 3 
                 Panelist 4 
                 Panelist 5 
                 Panelist 6 
               
               
                   
               
             
            
               
                 bitter 
                 bitter 
                 sour/bitter 
                 astringent 
                 astringent 
                 bitter 
               
               
                 sweet 
                 astringent 
                 sweet 
                 sweet 
                 sweet 
                 sweet 
               
               
                 astringent 
                 sweet 
                 cocoa 
                 sour 
                 sour 
                 sour 
               
               
                 roasted 
                   
                 tongue 
                 bitter 
                 bitter 
                 roasted 
               
               
                   
                   
                 drying 
                   
                   
                 cocoa 
               
               
                 sour 
                   
                   
                   
                 fruity 
                 astringent 
               
               
                   
               
            
           
         
       
     
     NuBana™ N100 green banana flour roasted for 30 minutes: 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                 Panelist 1 
                 Panelist 2 
                 Panelist 3 
                 Panelist 4 
                 Panelist 5 
                 Panelist 6 
               
               
                   
               
             
            
               
                 bitter 
                 astringent 
                 bitter 
                 bitter 
                   
                 bitter 
               
               
                 sweet 
                 burnt 
                 burnt 
                   
                   
                 sweet 
               
               
                   
                   
                   
                   
                   
                 roasted 
               
               
                 astringent 
                 sweet 
                 roasted 
                   
                   
                 sour 
               
               
                 roasted 
                   
                   
                   
                   
                   
               
               
                 cooked 
                   
                   
                   
                   
                   
               
               
                 sugar 
               
               
                   
               
            
           
         
       
     
     NuBana™ N100 green banana flour roasted for 40 minutes: 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                 Panelist 1 
                 Panelist 2 
                 Panelist 3 
                 Panelist 4 
                 Panelist 5 
                 Panelist 6 
               
               
                   
               
             
            
               
                 bitter 
                 bitter 
                 harsh 
                 bitter 
                 burnt 
                   
               
               
                   
                   
                 bitter 
                   
                 bitter 
                   
               
               
                 astringent 
                 astringent 
                 tongue 
                 BURNT 
                   
                   
               
               
                   
                   
                 burning 
                 burnt coffee 
                   
                   
               
               
                   
                   
                   
                 ground 
                   
                   
               
               
                   
                 hammy 
                   
                 Cross 
                 chewing a 
                 I feel like I 
               
               
                   
                   
                   
                 between 
                 smoked 
                 licked an 
               
               
                   
                   
                   
                 eating used 
                 cigarette 
                 ash tray 
               
               
                   
                   
                   
                 coffee 
                   
                   
               
               
                   
                   
                   
                 grounds and 
                   
                   
               
               
                   
                   
                   
                 cigarettes. 
               
               
                   
               
            
           
         
       
     
     NuBana™ N100 green banana flour roasted for 50 minutes: 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                 Panelist 1 
                 Panelist 2 
                 Panelist 3 
                 Panelist 4 
                 Panelist 5 
                 Panelist 6 
               
               
                   
               
             
            
               
                 astringent 
                 bitter 
                 bitter 
                 bitter burnt 
                 cannot 
                 cannot 
               
               
                   
                   
                   
                   
                 distinguish 
                 taste past 
               
               
                   
                   
                   
                   
                 so burnt and 
                 the smoky 
               
               
                   
                   
                   
                   
                 awful 
                 ashtray 
               
               
                 bitter 
                   
                   
                   
                   
                   
               
               
                 smoky 
               
               
                   
               
            
           
         
       
     
     Conclusions from Experiment 1: Shorter roasting times of the green banana flour may provide for more sour, fruity notes. As the green banana flour is roasted for longer periods of time, sour, fruity notes may be perceived less and new flavors develop, such as coffee flavor, roasted flavor, smoky and burnt. Thus, adjusting roasting times and temperatures of green banana flour in accordance with the present disclosure can result in a variety of sensory attributes. 
     Experiment 2 
     The trained panel, including a sensory expert, was presented with a control preparation containing cocoa powder and a test sample preparation containing NuBana™ N100 green banana flour roasted at 205° C. for the times shown below. All taste tests were conducted at room temperature. The panelists sampled preparations of the cocoa control and the roasted green banana flour prepared according to Food Product Example 2 above, wherein for the test samples NuBana™ N100 green banana flour was substituted for the cocoa powder. 
     The panel&#39;s sensory results from Experiment 2 are summarized as follows: 
     Cocoa Control: dairy, sweet, sour, bitter, astringent. 
     NuBana™ N100 green banana flour roasted for 15 minutes: sweet, dairy, sour, bitter, astringent, fruity. 
     NuBana™ N100 green banana flour roasted for 20 minutes: sweet, dairy, bitter, astringent sour, tongue drying sensation. 
     NuBana™ N100 green banana flour roasted for 30 minutes: dairy, sweet bitter, astringent, roasted, coffee. 
     NuBana™ N100 green banana flour roasted for 40 minutes: dairy, sweet, bitter, astringent, burnt, roasted, smoke. 
     Conclusions from Experiment 2: The NuBana™ N100 green banana flour roasted for 30-40 minutes at 205° C. produced roasted flavors that enhance a natural cocoa powder when blended into a dairy chocolate drink. 
     Experiment 3 
     All samples were prepared by the methods described in Food Product Example 3 above, wherein in the control sample cocoa powder was substituted for the roasted green banana flour. In this experiment, sensory attributes of the test sample containing roasted green banana flour were also measured on a “degree of difference” (DOD) scale, used to represent how similar the test sample was to the control sample, the scale operating from 1 to 7, 1 representing no difference and 7 representing significant difference. In this experiment the NuBana™ N100 green banana flour was roasted at 205° C. for 40 minutes and had a pH before roasting of about 4, and the green banana flour was approximately 6 months old. 
     Results 
     Control—12% Cocoa 
     
       
         
           
               
               
               
               
               
               
               
             
               
                   
               
               
                 Panelist 
                 Panelist 
                 Panelist 
                 Panelist 
                 Panelist 
                 Panelist 
                 Panelist 
               
               
                 1 
                 2 
                 3 
                 4 
                 5 
                 6 
                 7 
               
               
                   
               
             
            
               
                 sweet 
                 dairy 
                 sweet 
                 sweet 
                 sweet 
                 creamy 
                 low 
               
               
                   
                   
                   
                   
                   
                 dairy 
                 sweet 
               
               
                 dairy 
                 sweet 
                 dairy 
                 Dairy 
                 dairy 
                 sweet 
                 dairy 
               
               
                 cocoa 
                 cocoa 
                   
                 Cocoa 
                 cocoa 
                 low 
               
               
                   
                   
                   
                   
                   
                 cocoa 
               
               
                   
                   
                   
                   
                 slight 
               
               
                   
                   
                   
                   
                 plastic 
               
               
                   
               
            
           
         
       
     
     Test Sample 1— 
     10% replacement of the cocoa powder with roasted NuBana™ N100 green banana flour heated at 205° C. for 40 minutes. 
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                   
                   
               
               
                   
                 Panelist 1 
                 Panelist 2 
                 Panelist 3 
                 Panelist 4 
                 Panelist 5 
                 Panelist 6 
                 Panelist 7 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 sweet 
                 sweet 
                 sweet 
                 sweet 
                 sweet 
                 sweet 
                 slow 
               
               
                   
                   
                   
                   
                   
                   
                   
                 release 
               
               
                   
                   
                   
                   
                   
                   
                   
                 of 
               
               
                   
                   
                   
                   
                   
                   
                   
                 flavor 
               
               
                   
                 dairy 
                 cocoa 
                 dairy 
                 cocoa 
                 dairy 
                 creamy 
                 low 
               
               
                   
                   
                   
                   
                   
                   
                 dairy 
                 sweet 
               
               
                   
                 cocoa 
                   
                   
                   
                 cocoa 
                 low 
                 dairy 
               
               
                   
                   
                   
                   
                   
                   
                 cocoa 
               
               
                   
                   
                   
                 slow 
                 same as 
                 no off 
                 no 
                 slight 
               
               
                   
                   
                   
                 flavor 
                 control 
                 notes 
                 difference 
                 Palm 
               
               
                   
                   
                   
                 release 
                   
                   
                 from 
                 oil 
               
               
                   
                   
                   
                   
                   
                   
                 control 
               
               
                 DOD* 
                 2 
                 2 
                 3 
                 1 
                 3 
                 1 
                 3 
               
               
                   
               
               
                 *= Degree Of Difference 
               
            
           
         
       
     
     The sensory expert commented that Test Sample 1 was very comparable to the control formulation in that the average consumer would not likely be able to tell the difference. It had a slightly slower flavor release, less cocoa flavor and had slight palm oil notes at the end, rating it as a DOD of 4. 
     Test Sample 2— 
     10% replacement of the cocoa powder with roasted NuBana™ P500 (pre-gelatinized) green banana flour heated at 205° C. for 15 minutes 
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                   
                   
               
               
                   
                 Panelist 1 
                 Panelist 2 
                 Panelist 3 
                 Panelist 4 
                 Panelist 5 
                 Panelist 6 
                 Panelist 7 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                   
                 slow 
               
               
                   
                   
                   
                   
                   
                   
                   
                 flavor 
               
               
                   
                   
                   
                   
                   
                   
                   
                 release 
               
               
                   
                 Sweet 
                 cocoa 
                 sweet 
                 sweet 
                 dairy 
                 sweet 
                 sweet 
               
               
                   
                 Dairy 
                 sweet 
                 dairy 
                 chocolate 
                 sweet 
                 cocoa 
                 cocoa 
               
               
                   
                   
                   
                   
                 flavor 
               
               
                   
                 Cocoa 
                   
                 cocoa 
                   
                 cocoa 
               
               
                   
                   
                 slight 
                 More 
                   
                 mocha 
                 bitter 
               
               
                   
                   
                 bitter 
                 chocolate 
                   
                 aftertaste 
                 aftertaste 
               
               
                   
                   
                   
                 flavor 
               
               
                 DOD* 
                 2 
                 4 
                 4 
                 3 
                 4 more 
                 4 
               
               
                   
                   
                   
                   
                   
                 flavor 
               
               
                   
               
               
                 *= Degree Of Difference 
               
            
           
         
       
     
     The sensory expert commented that Test Sample 2 tasted like flavor was added to the compound coating. In addition, Test Sample 2 had interesting flavor built over time, providing an attribute greater than the cocoa powder that had a roasted coffee-like attribute. 
     Test Sample 3— 
     25% replacement of the cocoa powder with roasted NuBana™ N100 green banana flour heated at 205° C. for 40 minutes 
     
       
         
           
               
               
               
               
               
               
               
             
               
                   
                   
               
               
                   
                 Panel- 
                 Panel- 
                 Panel- 
                 Panel- 
                 Panel- 
                 Panel- 
               
               
                   
                 ist 1 
                 ist 2 
                 ist 3 
                 ist 4 
                 ist 5 
                 ist 5 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 sweet 
                 cocoa 
                 sweet 
                 sweet 
                 dairy 
                 sweet 
               
               
                   
                 cocoa 
                 sweet 
                 cocoa 
                 cocoa 
                 sweet 
                 cocoa 
               
               
                   
                   
                   
                   
                 bitter 
                 cocoa 
               
               
                   
                   
                   
                 Slight 
                 more 
                 mocha 
                 bitter 
               
               
                   
                   
                   
                 tongue 
                 overall 
                 aftertaste 
                 aftertaste 
               
               
                   
                   
                   
                 drying 
                 initial 
               
               
                   
                   
                   
                   
                 flavor 
               
               
                 DOD* 
                 4 
                 4 
                 4 
                 5 
                 5 
                 5 
               
               
                   
               
               
                 *= Degree Of Difference 
               
            
           
         
       
     
     The sensory expert commented that Test Sample 3 had high end attributes like high end cocoa powders. In addition, Test Sample 3 had a longer flavor release with more intensive chocolate flavor, providing for an intensified chocolate flavor at the front end and at the back end. 
     Test Sample 4— 
     50% replacement of the cocoa powder with roasted NuBana™ N100 green banana flour heated at 205° C. for 15 minutes 
     
       
         
           
               
               
               
               
               
               
               
             
               
                   
                   
               
               
                   
                 Panel- 
                 Panel- 
                 Panel- 
                 Panel- 
                 Panel- 
                 Panel- 
               
               
                   
                 ist 1 
                 ist 2 
                 ist 3 
                 ist 4 
                 ist 5 
                 ist 6 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 sweet 
                 cocoa 
                 sweet 
                 roasted 
                 sweet 
                 sweet 
               
               
                   
                 cocoa 
                 chocolate 
                 cocoa 
                 cocoa 
                   
                 cocoa 
               
               
                   
                   
                   
                   
                 burnt 
                 cocoa 
               
               
                   
                   
                   
                   
                 cocoa 
               
               
                   
                   
                   
                   
                 sweet 
               
               
                   
                 slow 
                   
                 bitter 
                 astringent 
                 smoky/ 
                 burnt 
               
               
                   
                 flavor 
                   
                   
                   
                 hammy 
               
               
                   
                 release 
               
               
                   
                 chewy 
                 grainy 
                   
                   
                 higher 
                 granular 
               
               
                   
                 texture 
                   
                   
                   
                 finest 
               
               
                 DOD* 
                 5 
                 5 
                 5 
                 6 
                 6 
                 5 
               
               
                   
               
               
                 *= Degree of difference 
               
            
           
         
       
     
     The sensory expert commented that the larger particle size of Test Sample 4 impacted flavor release, and a different flavor attribute was introduced at the end resembling burnt caramelized sugar, the flavor ending slightly bitter. Some cocoa powders share these attributes. 
     Test Sample 5— 
     25% reduction in total cocoa powder. 
     Because more intense chocolate flavor was found at 25% cocoa replacement (from the results in Test Sample 3, above), the formulation was repeated for testing designed to identify if less total cocoa powder could produce a similar flavor intensity. 
     
       
         
           
               
               
               
               
               
               
               
             
               
                   
                   
               
               
                   
                 Panel- 
                 Panel- 
                 Panel- 
                 Panel- 
                 Panel- 
                 Panel- 
               
               
                   
                 ist 1 
                 ist 2 
                 ist 3 
                 ist 4 
                 ist 5 
                 ist 6 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 sweet 
                 sweet 
                 sweet 
                 sweet 
                 sweet 
                 sweet 
               
               
                   
                   
                   
                 cocoa 
               
               
                   
                   
                 low 
                   
                 low 
                 cocoa 
                 low 
               
               
                   
                   
                 cocoa 
                   
                 bitter 
                   
                 cocoa 
               
               
                   
                 sweet 
                   
                 sweet 
                 sweet 
                 sweet 
               
               
                   
                   
                   
                 mostly 
               
               
                 DOD* 
                 5 
                 4 
                 5 
                 5 
                 5 
                 5 
               
               
                   
               
               
                 *= Degree of difference 
               
            
           
         
       
     
     The sensory expert commented that the Test Sample 5 had less cocoa flavor, and was sweeter, but had no off notes. The sample was comparable to a carob like product with a reduced overall cocoa flavor. The sensory expert expressed surprise that no off flavors were noted. 
     Conclusions from Experiment 3: The degree of sensory difference observed by the panelists generally increased as more cocoa powder was replaced with roasted green banana flour. 
     However, even at relatively high levels of cocoa replacement, panelists and the sensory expert identified several sensory attributes and accompanying intensities in common with cocoa or chocolate. 
     Experiment 4 
     All control and test samples were prepared by the methods described in Food Product Example 3 above, wherein roasted green banana flour (NuBana™ GBF P500) was used at 10, 25 and 50% replacement of the cocoa. In this experiment, sensory attributes of the test samples containing roasted green banana flour were also measured by five panelists on a “degree of difference” (DOD) scale, used to represent how similar the test sample was to the control sample, the scale operating from 1 to 7, 1 representing no difference and 7 representing significant difference. In this experiment the NuBana™ P500 green banana flour was roasted at 205° C. for 40 minutes and had a pH before roasting of about 6.57, and the green banana flour was approximately two weeks old. The results of the taste panel are shown below. 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                   
                   
                 Test Sample 1, 
                 Test Sample 2,  
                   
               
               
                   
                   
                 10% cocoa 
                 25% cocoa 
                   
               
               
                   
                   
                 replacement 
                 replacement 
                 Test Sample 3, 50% 
               
               
                   
                   
                 with Roasted 
                 with Roasted 
                 cocoa replacement 
               
               
                   
                   
                 NuBana ™ 
                 NuBana ™ 
                 with Roasted 
               
               
                   
                 Control 
                 P500 green 
                 P500 green 
                 NuBana ™ P500 
               
               
                   
                 Sample 
                 banana flour 
                 banana flour 
                 green banana flour 
               
               
                   
               
             
            
               
                 Panelist 1 
                   
                   
                   
                   
               
               
                 Color 
                 Medium 
                 Medium 
                 Medium 
                 Medium Brown 
               
               
                   
                 Brown 
                 Brown 
                 Brown 
                   
               
               
                 Flavor 
                 Sweet 
                 Sweet 
                 Sweet 
                 Sweet 
               
               
                   
                 Cocoa 
                 Cocoa 
                 Mocha 
                 Cocoa 
               
               
                   
                 Dairy 
                 Dairy 
                 Cocoa 
                 Coffee ground like 
               
               
                   
                   
                   
                   
                 bitter 
               
               
                   
                   
                   
                 Dairy 
                 Astringent 
               
               
                 DOD 
                   
                 2 
                 4 
                 5 
               
               
                 Comments 
                   
                   
                   
                 Slow Flavor 
               
               
                   
                   
                   
                   
                 Release, 
               
               
                   
                   
                   
                   
                 Granular mouthfeel 
               
               
                 Panelist 2 
                   
                   
                   
                   
               
               
                 Color 
                 Medium 
                 Medium 
                 Medium 
                 Medium Brown 
               
               
                   
                 Brown 
                 Brown 
                 Brown 
                   
               
               
                 Flavor 
                 Sweet 
                 Sweet 
                 Sweet 
                 Sweet 
               
               
                   
                 Cocoa 
                 Cocoa 
                 Mocha 
                 Mocha 
               
               
                   
                 Dairy 
                 Dairy 
                 Cocoa 
                 Cocoa 
               
               
                   
                   
                   
                 Dairy 
                 Bitter 
               
               
                 DOD 
                   
                 2 
                 4 
                 5 
               
               
                 Comments 
                   
                   
                   
                 Tongue drying 
               
               
                   
                   
                   
                   
                 astringent 
               
               
                 Panelist 3 
                   
                   
                   
                   
               
               
                 Color 
                 Medium 
                 Medium 
                 Medium 
                 Medium Brown 
               
               
                   
                 Brown 
                 Brown 
                 Brown 
                   
               
               
                   
                 Sweet 
                 Sweet 
                 Sweet 
                 Sweet 
               
               
                 Flavor 
                 Cocoa 
                 Cocoa 
                 Cocoa 
                 Cocoa 
               
               
                   
                 Dairy 
                 Dairy 
                 Dairy 
                 Bitter 
               
               
                   
                   
                   
                   
                 Astringent 
               
               
                   
                   
                   
                   
                 Tasted something 
               
               
                   
                   
                   
                   
                 else added vanilla 
               
               
                   
                   
                   
                   
                 mocha 
               
               
                 DOD 
                   
                 2 
                 3 
                 5 
               
               
                 Comments 
                   
                   
                   
                 Slow Flavor 
               
               
                   
                   
                   
                   
                 Release, 
               
               
                   
                   
                   
                   
                 Granular mouthfeel 
               
               
                 Panelist 4 
                   
                   
                   
                   
               
               
                 Color 
                 Medium 
                 Medium 
                 Medium 
                 Medium Brown 
               
               
                   
                 Brown 
                 Brown 
                 Brown 
                   
               
               
                 Flavor 
                 Sweet 
                 Sweet 
                 Sweet 
                 Sweet 
               
               
                   
                 Cocoa 
                 Cocoa 
                 Cocoa 
                 Mocha 
               
               
                   
                 Dairy 
                 Dairy 
                 Dairy 
                 Cocoa 
               
               
                   
                   
                   
                   
                 Bitter 
               
               
                   
                   
                   
                 Higher cocoa 
                 Astringent 
               
               
                   
                   
                   
                 notes 
                   
               
               
                 DOD 
                   
                 2 
                 3 
                 5 
               
               
                 Comments 
                   
                   
                   
                 Slow Flavor 
               
               
                   
                   
                   
                   
                 Release, 
               
               
                   
                   
                   
                   
                 Granular mouthfeel 
               
               
                 Panelist 5 
                   
                   
                   
                   
               
               
                 Color 
                 Medium 
                 Medium 
                 Medium 
                 Medium Brown 
               
               
                   
                 Brown 
                 Brown 
                 Brown 
                   
               
               
                 Flavor 
                 Sweet 
                 Sweet 
                 Sweet 
                 Sweet 
               
               
                   
                 Cocoa 
                 Cocoa 
                 Mocha/cocoa 
                 Mocha 
               
               
                   
                 Dairy 
                 Dairy 
                 Dairy 
                 Cocoa 
               
               
                   
                   
                   
                   
                 Bitter 
               
               
                   
                   
                   
                   
                 Astringent 
               
               
                 DOD 
                   
                 1 
                 4 
                 5 
               
               
                 Comments 
                   
                 Same product 
                 Added mocha 
                 Slow Flavor 
               
               
                   
                   
                   
                 to the cocoa 
                 Release, 
               
               
                   
                   
                   
                 flavor 
                 Gritty. Harsh 
               
               
                   
                   
                   
                   
                 aftertaste 
               
               
                   
               
            
           
         
       
     
     Conclusion from Experiment 4: roasted green banana flour with a higher pre-roasting pH gave better taste profiles without the development of the burnt and off tastes that were seen in the lower pH samples (e.g., Experiment 3). In addition, at the higher pH more complex flavor attributes were noted, resembling a higher end cocoa product. 
     Experiment 5 
     A compound coating was made using the standard ball mill procedure with the ingredients from Food Product Example 3 above, but without the cocoa or roasted green banana flour. The batch was split and cocoa powder was added at 12% usage for the control and a blend of cocoa powder and roasted NuBana™ P500 green banana flour (roasted at 205° C. for 40 minutes) was used for each of three test samples as shown in the table below. The powders were added and mixed in a standard baking mixer. The compound samples were molded at 37° C. in wafer molds and put in a freezer for 5 minutes to set up before demolding. Viscosities were measured for the test sample and the control samples using a HA Brookfield™ viscometer at 20 RPM, #27 Spindle at 60° C. 
     Results: 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 viscosities of formulated chocolate coating 
               
            
           
           
               
               
            
               
                   
                 Viscosity HA Brookfield at 
               
               
                   
                 20 RPM, #27 Spindle at 50° C. 
               
               
                 Sample 
                 (% torque) 
               
               
                   
               
               
                 Control-12% cocoa powder 
                 25 
               
               
                 Test Sample 6-10.8% cocoa powder, 
                 35 
               
               
                 1.2% roasted NuBana ™ P500 green 
                   
               
               
                 banana flour 
                   
               
               
                 Test Sample 7-9% cocoa powder, 3% 
                 38 
               
               
                 roasted NuBana ™ P500 green banana 
                   
               
               
                 flour 
                   
               
               
                 Test Sample 8-6% cocoa powder, 6% 
                 36 
               
               
                 roasted NuBana ™ P500 green banana 
                   
               
               
                 flour 
                   
               
               
                   
               
            
           
         
       
     
     Conclusions from Experiment 5: The viscosities of the compound coating sample tests were averaging 10% higher than the control sample. Though not highly significant, this margin could be attributed to the 12% cocoa butter that is intrinsically in cocoa powder or could be due to the differences in particle size distribution between the control and test samples. Furthermore, viscosity could be easily adjusted by the addition of 1-2% palm kernel oil (e.g., the held back 2% of the oil from the Food Product Example 3 method). With respect to color results, the color on all samples was medium to dark brown. There was not a noticeable difference in color between the control and test samples. 
     Experiment 6 
     All brownie samples were prepared by the methods described in Food Product Example 4 above. Sensory attributes of each of the Test Samples 9, 10 and 11 containing roasted green banana flour were measured by a sensory panel on a “degree of difference” (DOD) scale compared to the control, the scale operating from 1 to 7, 1 representing no difference and 7 representing significant difference. 
     The overall results of the sensory evaluation were as follows: 
     For Test Sample 9 (10% cocoa replacement), the panel noted an overall DOD of 1, and detected no perceivable difference in flavor, color, or other appearance characteristics, as compared with the control. Based on these results, it was concluded that the average consumer would not detect a difference between a brownie made according to the control formulation and a brownie made according to the Test Sample 9 formulation. 
     For Test Sample 10 (25% cocoa replacement), the panel noted an overall DOD of 2, including a texture slightly less chewy than the control, and no perceivable difference in color or other appearance characteristics, as compared with the control. Based on these results, it was concluded that the average consumer would not detect a difference between a brownie made according to the control formulation and a brownie made according to the Test Sample 10 formulation. 
     For Test Sample 11 (50% cocoa replacement), the panel noted an overall DOD of 4, including a less baked chocolate brownie flavor and the presence of a cooked fruit flavor as compared with the control. There was no perceivable difference in color as compared with the control. Based on these results, it was concluded that the average consumer may or may not detect a difference between a brownie made according to the control formulation and a brownie made according to the Test Sample 11 formulation. 
     Experiment 7 
     All chocolate coating compound samples were prepared by the methods described in Food Product Example 5 above. Sensory attributes of each of the Test Samples 12, 13 and 14 containing roasted green banana flour were measured by a sensory panel on a “degree of difference” (DOD) scale compared to the control, the scale operating from 1 to 7, 1 representing no difference and 7 representing significant difference. 
     For Test Sample 12 (10% cocoa replacement), the panel noted an overall DOD of 1, and detected no perceivable difference in flavor, color, or other appearance characteristics, as compared with the control. Based on these results, it was concluded that the average consumer would not detect a difference between a chocolate coating compound made according to the control formulation and a chocolate coating compound made according to the Test Sample 12 formulation. 
     For Test Sample 13 (25% cocoa replacement), the panel noted an overall DOD of 2, including a texture slightly less chewy than the control, and no perceivable difference in color or other appearance characteristics, as compared with the control. Based on these results, it was concluded that the average consumer would not detect a difference between a chocolate coating compound made according to the control formulation and a chocolate coating compound made according to the Test Sample 13 formulation. 
     For Test Sample 14 (50% cocoa replacement), the panel noted an overall DOD of 4, including a less overall cocoa flavor as compared with the control, and also the presence of a cooked fruit flavor. In addition, a slower melt and slower flavor release as compared with the control were noted. 
     Based on the results of the Test Samples 12, 13 and 14, it was concluded that Samples 12 and 13 were successful at replacing cocoa powder without a detectable difference from the control, while Sample 14 had a noticeable difference both in flavor and texture. 
     Experiment 8—Effect of Time and Temperature on Color and Taste Profile 
     Additional sensory testing was done to look at the degree of difference (DOD) between roasted NuBana™ N100 green banana flour roasted at 205° C. for 40 minutes and NuBana™ N100 roasted at different roasting temperatures and times to determine one or more roasting temperatures and/or roasting times of green banana flour for optimizing its color and/or taste profiles. 
     Table 5 below provides the roasting temperatures and times for four different samples (Samples 15-18) of NuBana™ N100, and the corresponding resulting color (Pantone #) as measured according to the Pantone Color Matching System (Pantone Inc. 590 Commerce Blvd., Carlstadt, N.J. 07072-3098). The Pantone Color Matching System is largely a standardized color reproduction system. It should be appreciated that, by standardizing the colors in this way, different manufacturers of green banana flour in different locations can all refer to the same standard color references to ensure product consistency. 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 5 
               
               
                   
                   
               
               
                   
                   
                 Roasting temp/time 
                 Pantone # (color) 
               
               
                   
                   
               
             
            
               
                   
                 Sample 15 
                 205° C./40 min 
                 732 U 
               
               
                   
                 (control) 
                   
                   
               
               
                   
                 Sample 16 
                 218° C./20 min 
                 464 U 
               
               
                   
                 Sample 17 
                 218° C./30 min 
                 732 U 
               
               
                   
                 Sample 18 
                 190° C./60 min 
                 732 U 
               
               
                   
                   
               
            
           
         
       
     
     To test the sensory differences between and among Samples 15-18, one tablespoon of each sample flour was mixed into 350 ml of room temperature water. The samples were evaluated according to the protocol set forth above in Example 1, with DOD evaluated by comparing each of samples 16-18 to sample 15 (the control). 
     With respect to Sample 16, the panel noted an overall DOD of 5, and the sample had an aroma of legume and no roasted notes. The sample also had green flavor notes, basic sour and legume notes and a bitter aftertaste. 
     With respect to Samples 17 and 18, the panel noted a DOD of 2, there being little to no perceivable difference compared with the control (Sample 15). 
     Based on the results of Experiment 8, it was concluded that a range of temperatures/roasting times can be used to prepare the same or similar color/taste profiles for roasted NuBana™ N100 green banana flour for purposes of using it as an effective cocoa substitute. 
     The Pantone Color Matching System was also used define colors associated with further green banana flour samples. Tables 6 and 7 below provides Pantone # index for different roasting times and temperatures for both NuBana™ N100 green banana flour and NuBana™ P500 green banana flour. This will allow for a quick visual evaluation and reference for samples made at different time/temperature profiles. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Pantone #&#39;s 
               
            
           
           
               
               
               
            
               
                   
                 Green 
                   
               
               
                   
                 Banana 
               
               
                 Temp 
                 Flour 
                 Roasting time 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                 (° C.) 
                 Type 
                 15 min 
                 30 min 
                 40 min 
                 60 min 
                 75 min 
                 90 min 
                 120 min 
                 150 min 
               
               
                   
               
               
                 162 
                 N100 
                   
                 7502U 
                   
                 7502U 
                   
                 465U 
                 465U 
                 464U 
               
               
                   
                 P500 
                   
                   
                   
                 465U 
                   
                 464U 
                 464U 
               
               
                 190 
                 N100 
                 7502U 
                 464U 
                 732U 
                 732U 
                 732U 
               
               
                   
                 P500 
                 7502U 
                 464U 
                 732U 
                 732U 
                 469U 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Pantone #&#39;s 
               
            
           
           
               
               
               
            
               
                   
                 Green 
                   
               
               
                   
                 Banana 
                   
               
               
                 Temp 
                 Flour 
                 Roasting time 
               
            
           
           
               
               
               
               
               
               
            
               
                 (° C.) 
                 Type 
                 15 min  
                 20 min 
                 30 min 
                 40 min 
               
               
                   
               
               
                 205° C. 
                 N100 
                 7502 U 
                 465 U 
                 464 U 
                 732 U 
               
               
                   
                 P500 
                 7502 U 
                 464 U 
                 732 U 
                 469 U 
               
               
                 218° C. 
                 N100 
                 7502 U 
                 464 U 
                 732 U 
                   
               
               
                   
                 P500 
                  465 U 
                 464 U 
                 469 U 
                   
               
               
                 232° C. 
                 N100 
                  465 U 
                 732 U 
                 732 U 
                   
               
               
                   
                 P500 
                  465 U 
                 464 U 
                 732 U 
               
               
                   
               
            
           
         
       
     
     Based on the color and all of the sensory evaluations described above, it was concluded that there is some correlation between the color and the desirable flavor notes that best correspond to the chocolate taste. That is, the roasted banana flours having a color more closely resembling cocoa tended to carrying more cocoa-like flavor notes. 
     As used herein, the phrase “at least one and up to six or more” includes at least one, at least two, at least three, at least four, at least five, and at least 6, and more than 6. 
     The various embodiments described above are provided by way of illustration only and should not be construed to limit the claims attached hereto. Those skilled in the art will readily recognize various modifications and changes that may be made without following the example embodiments and applications illustrated and described herein, and without departing from the true spirit and scope of the following claims.