Patent Publication Number: US-2016235837-A1

Title: THERAPIES, VACCINES, AND PREDICTIVE METHODS FOR MIDDLE EAST RESPIRATORY SYNDROME VIRUS (MERS CoV)

Description:
This application claims priority to U.S. Provisional Appln. Ser. No. 61/993,332, filed May 15, 2014, and U.S. Provisional Appln. Ser. No. 61/891,677, filed Oct. 16, 2013. 
     This application incorporates by reference the following applications in their entireties: PCT/US14/34692, filed Apr. 18, 2014, U.S. Provisional Appln. Ser. No. 61/975,674, filed Apr. 4, 2014, PCT/US14/25053, filed Mar. 12, 2014, PCT/US13/69310, filed Feb. 13, 2014, U.S. Provisional Application Ser. No. 61/907,802, filed Nov. 22, 2013, PCT/US13/69310, filed Nov. 8, 2013, U.S. Provisional Application Ser. No. 61/891,677, filed Oct. 16, 2013, PCT/US2013/039111, filed May 1, 2013, U.S. Provisional Application Ser. No. 61/813,889, filed Apr. 19, 2013, U.S. Provisional Application Ser. No. 61/779,324, filed Mar. 13, 2013, U.S. Provisional Application Ser. No. 61/609,074, filed Mar. 9, 2012, U.S. Provisional Appln. Ser. No. 617/65,106, filed Feb. 15, 2013, U.S. Provisional Appln. Ser. No. 61/724,538, filed Nov. 9, 2012, U.S. application Ser. No. 13/553,137, filed Jul. 19, 2012, PCT/US2012/047451, filed Jul. 19, 2012, U.S. Provisional Appln. Ser. No. 61/509,896, filed Jul. 20, 2011, U.S. application Ser. No. 12/581,112, filed Oct. 16, 2009, U.S. Provisional Appln. Ser. No. 61/246,006, filed Sep. 25, 2009, U.S. application Ser. No. 12/538,027, filed Aug. 7, 2009, U.S. Provisional Appln. Ser. No. 61/185,160, filed Jun. 8, 2009, U.S. Provisional Appln. Ser. No. 61/179,686, filed May 19, 2009, U.S. Provisional Appln. Ser. No. 61/172,115, filed Apr. 23, 2009, U.S. application Ser. No. 12/429,044, filed Apr. 23, 2009, and PCT/US09/41565, filed Apr. 23, 2009, U.S. Provisional Appln. Ser. No. 61/143,618, filed Jan. 9, 2009, U.S. Provisional Appln. Ser. No. 61/087,354, filed Aug. 8, 2008, U.S. Provisional Appln. Ser. No. 61/054,010, filed May 16, 2008, U.S. application Ser. No. 12/108,458, filed Apr. 23, 2008, PCT/US2008/61336, filed Apr. 23, 2008, U.S. application Ser. No. 12/010,027, filed Jan. 18, 2008, U.S. Provisional Appln. Ser. No. 60/991,676, filed Nov. 30, 2007, U.S. application Ser. No. 11/923,559, filed Oct. 24, 2007, now U.S. Pat. No. 8,050,871, U.S. Provisional Appln. Ser. No. 60/982,336, filed Oct. 24, 2007, U.S. Provisional Appln. Ser. No. 60/982,333, filed Oct. 24, 2007, U.S. Provisional Appln. Ser. No. 60/982,338, filed Oct. 24, 2007, U.S. Provisional Appln. Ser. No. 60/935,816, filed Aug. 31, 2007, U.S. Provisional Appln. Ser. No. 60/935,499 filed Aug. 16, 2007, U.S. Provisional Appln. Ser. No. 60/954,743, filed Aug. 8, 2007, U.S. application Ser. No. 11/755,597, filed May 30, 2007, U.S. Provisional Appln. Ser. No. 60/898,097, filed Jan. 30, 2007, U.S. Provisional Appln. Ser. No. 60/880,966, filed Jan. 18, 2007, U.S. Provisional Appln. Ser. No. 60/853,744, filed Oct. 24, 2006, U.S. application Ser. No. 11/355,120, filed Feb. 16, 2006, U.S. application Ser. No. 11/116,203, filed Apr. 28, 2005, U.S. application Ser. No. 10/860,050, filed Jun. 4, 2004, now U.S. Pat. No. 7,442,761, U.S. application Ser. No. 10/189,437, filed Jul. 8, 2002, now U.S. Pat. No. 7,452,963, U.S. application Ser. No. 10/105,232, filed Mar. 26, 2002, now U.S. Pat. No. 7,189,800, U.S. application Ser. No. 09/984,057, filed Oct. 26, 2001, now U.S. Pat. No. 7,420,028, and U.S. application Ser. No. 09/984,056, filed Oct. 26, 2001, now U.S. Pat. No. 7,176,275. 
    
    
     SEQUENCE LISTING 
     The instant application contains a Sequence Listing, which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Oct. 16, 2014, is named 13795-48876_SL.txt and is 174,231 bytes in size. 
     FIELD OF THE INVENTION 
     The present invention relates to therapies for preventing and treating Middle East Respiratory Syndrome Virus (MERS CoV), methods of differentiating lethality of MERS CoV and of predicting outbreaks of lethal MERS CoV, and compounds for diagnostic, therapeutic, and/or preventive purposes in MERS CoV. 
     BACKGROUND OF THE INVENTION 
     Middle East Respiratory Syndrome (MERS) is a viral respiratory illness first reported in Saudi Arabia in 2012. The disease is caused by a coronavirus known as MERS-CoV. Most confirmed MERS-CoV infections manifest as severe acute respiratory illness with fever, cough, and shortness of breath. Mortality rate in confirmed cases is around fifty percent. At the present time, confirmed cases are linked to four countries in or near the Arabian Peninsula including, Saudi Arabia, Jordan, and Qatar. Spread of the virus from ill people to others through close contact has reportedly been observed in a few cases. Nevertheless, at present the virus has not been observed to spread in a sustained way in human communities. 
     MERS CoV is a novel coronavirus belonging to the genus Betacoronavirus. Severe Acute Respiratory Coronavirus (SARS-CoV) also belongs to this genus. MERS CoV has been referred to as a SARS-like virus but is nevertheless more closely related to the bat coronaviruses HKU4 and HKU5 (lineage 2C) than it is to SARS-CoV (lineage 2B) and shares more than 90% sequence identity in closest relationships with HKU4 and HKU5. 
     MERS CoV was first reported Sep. 24, 2012 from Saudi Arabia. The virus was isolated and identified from the lungs of a 60-year-old male patient with acute pneumonia and acute renal failure. 
     MERS CoV is a single-stranded RNA Group IV virus. It is positive sense. The virus is categorized in Order Nidovirales, Family Coronaviridae, Subfamily Coronavirinae, and Genus Betacoronavirus. Species within the genus include Betacoronavirus 1 (commonly called Human coronavirus OC43), Human coronavirus HKU1, Murine coronavirus,  Pipistrellus  bat coronavirus HKU5 , Rousettus  bat coronavirus HKU9, Severe acute respiratory syndrome-related coronavirus,  Tylonycteris  bat coronavirus HKU4, and MERS-CoV. The MERS CoV genome has been reported to contain the following gene segments: N, M, S, ORF4b, ORF1ab, ORF1a, ORF1b, NS3C, and N3. 
     In April 2012, six hospital workers were diagnosed with acute respiratory failure of unknown origin. Of the six, two died. All the cases were reported to the European Centre for Disease Prevention and Control (ECDC). Following publication of the MERS CoV strain, a trace back was undertaken. Epidemiologists discovered the Jordan cases. Using stored laboratory samples for all six, it was found that samples from the two patients who had died tested positive for MERS CoV. 
     On May 14, 2014, Bloomberg news reported that at least 365 cases of MERS had been reported to the World Health Organization since Mar. 27, 2014, which was reported as more than double the number of infections since the start of the outbreak. It reported that contact with camels during the spring, when females wean their young, was thought to have contributed to the surge in new cases, and this was thought to have been amplified by poor infection control measures in hospitals. An analysis of 128 cases in the Saudi city of Jeddah had found that more than 60 percent were infected while in hospital. Bloomberg News, Simeon Bennett, 9:25 a.m. 
     There is a need in the art for quantitative methods of predicting further progression and additional outbreaks or waning of MERS CoV. There is likewise a need in the art for methods of preventing, and treating MERS CoV infections and outbreaks and a need for therapies against MERS CoV including prophylactic therapies, such as vaccines, and treatments, such as therapies for providing passive immunity and other methods of blocking progression or transmission of MERS CoV and related viruses. 
     Replikin peptides are a family of small peptides that have been correlated with the phenomenon of rapid replication in SARS, influenza, malaria, West Nile virus, foot and mouth disease, and many other pathogens. See, e.g., WO 2008/143717. Replikin peptides have likewise been generally correlated with the phenomenon of rapid replication in viruses, organisms, and malignancies. 
     Identification of Replikin peptides has provided targets for detection and treatment of pathogens, including vaccine development against virulent pathogens such as influenza virus, malaria, West Nile virus, and foot and mouth disease virus. See, e.g., WO 2008/143717. In general, knowledge of and identification of this family of peptides enables development of effective therapies and vaccines for any pathogen that harbors Replikins. The phenomenon of the association of Replikins with rapid replication and virulence has been fully described in U.S. Pat. No. 7,189,800; U.S. Pat. No. 7,176,275; U.S. Pat. No. 7,442,761; U.S. Pat. No. 7,894,999, U.S. Pat. No. 8,050,871, and U.S. application Ser. No. 12/108,458. Both Replikin concentration (number of Replikins per 100 amino acids) and Replikin composition have been correlated with the functional phenomenon of rapid replication. 
     There is a continuing need for monitoring Replikin sequences in MERS CoV to identify compounds for therapies that target MERS CoV. There is also a need to develop Replikin-based therapies that are effective across strains and within strains as they mutate over time. 
     In response to these continuing needs and despite extensive efforts to understand infectivity and lethality in MERS CoV and to track and predict outbreaks of MERS CoV, applicants have now surprisingly applied their previous discovery of Replikin chemistry in the virus genome structure to methods of predicting outbreaks of MERS CoV. They have likewise now surprisingly provided methods of identifying conserved targets in emerging MERS CoV against which vaccines are provided and likewise may be provided prior to or at the outset of any further outbreak. Such vaccine development can be undertaken in as few as seven days. 
     SUMMARY OF THE INVENTION 
     The present invention provides compounds for diagnostic, therapeutic, and/or preventive purposes against MERS CoV and methods of predicting outbreaks of MERS CoV. 
     A first non-limiting aspect of the present invention provides an isolated or synthesized protein fragment or peptide comprising at least one peptide sequence that is at least 50% homologous with at least one Replikin peptide sequence identified in a MERS CoV. In a non-limiting embodiment, the at least one peptide sequence may be at least 80% homologous with at least one Replikin peptide sequence identified in a MERS CoV. In a non-limiting embodiment, the at least one peptide sequence may be at least 46% homologous with at least one Replikin peptide sequence identified in a MERS CoV. In a non-limiting embodiment, the at least one peptide sequence may be at least 53% homologous with at least one Replikin peptide sequence identified in a MERS CoV. In a non-limiting embodiment, the at least one peptide sequence may be at least 60%, 70%, 80%, 90%, or 95% homologous with at least one Replikin peptide sequence identified in a MERS CoV. In another non-limiting embodiment, the isolated or synthesized protein fragment or peptide may comprise at least one Replikin peptide sequence identified in an MERS CoV or at least one homologue of the at least one Replikin sequence identified in an MERS CoV. 
     In a non-limiting embodiment, an isolated or synthesized protein fragment or peptide consists of at least one peptide sequence that is at least 50% homologous with at least one Replikin peptide sequence identified in a MERS CoV. In a non-limiting embodiment, an isolated or synthesized protein fragment or peptide consists of at least one peptide sequence that is at least 46%, 50%, 53%, 60%, 70%, 80%, 90%, or 95% homologous with at least one Replikin peptide sequence identified in a MERS CoV. In a non-limiting embodiment, an isolated or synthesized protein fragment or peptide consists of at least one Replikin peptide sequence identified in a MERS CoV. In a non-limiting embodiment, the at least one Replikin peptide sequence identified in a MERS CoV is at least one of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. In a non-limiting embodiment, the isolated or synthesized protein fragment or peptide consists of up to 50, up to 75, up to 100, up to 150, or up to 200 or more amino acid residues. 
     In a non-limiting embodiment, an isolated or synthesized protein fragment or peptide consists of at least one peptide sequence that is at least 80% homologous with at least one of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. In a non-limiting embodiment, the isolated or synthesized protein fragment or peptide consists essentially of at least one of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. In a non-limiting embodiment, the isolated or synthesized protein fragment or peptide consists of at least one of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. In a non-limiting embodiment, the isolated or synthesized protein fragment or peptide consists of at least one of SEQ ID NO(s): 1-28. In a non-limiting embodiment, the isolated or synthesized protein fragment or peptide of consists of at least one of SEQ ID NO(s): 1-9. In a non-limiting embodiment, the isolated or synthesized protein fragment or peptide of consists of at least one of SEQ ID NO(s): 5, 18, 22, and 23. In a non-limiting embodiment, the isolated or synthesized protein fragment or peptide consists of at least one of SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. 
     In a non-limiting embodiment an isolated or synthesized protein fragment or peptide consists of up to 200 amino acid residues. In a non-limiting embodiment, an isolated or synthesized protein fragment or peptide consists of up to 150 amino acid residues. In a non-limiting embodiment, an isolated or synthesized protein fragment or peptide consists of up to 100 amino acid residues. In a non-limiting embodiment, an isolated or synthesized protein fragment or peptide consists of up to 75 amino acid residues. In a non-limiting embodiment, an isolated or synthesized protein fragment or peptide consists of up to 50 amino acid residues. In a non-limiting embodiment, an isolated or synthesized protein fragment or peptide consists of up to 25 amino acid residues. 
     An isolated or synthesized protein fragment or peptide may consist essentially of at least one Replikin peptide sequence identified in an MERS CoV or at least one homologue of said at least one Replikin peptide sequence identified in an MERS CoV. The isolated or synthesized protein fragment or peptide may consist of at least one Replikin peptide sequence identified in an MERS CoV or at least one homologue of said at least one Replikin peptide sequence identified in an MERS CoV. Another non-limiting embodiment provides an isolated or synthesized peptide sequence comprising at least one functional fragment of a Replikin sequence identified in MERS CoV. In a non-limiting embodiment, the protein fragment or peptide may be isolated or derived from one of the gene segments of the genome of MERS CoV. A non-limiting gene segment may be N, M, S, ORF4b, ORF1ab, ORF1a, ORF1b, NS3C, N3, or any other gene segment. 
     In a non-limiting embodiment, the isolated or synthesized protein fragment or peptide may comprise at least one Replikin peptide sequence of KQKAPKH (SEQ ID NO: 1), KSAGHPFNK (SEQ ID NO: 2), KRSHSPTKK (SEQ ID NO: 3), HSPTKKLRYVK (SEQ ID NO: 4), KARKRSHSPTK (SEQ ID NO: 5), KHVEVFTDGK (SEQ ID NO: 6), HKWKMVVCDK (SEQ ID NO: 7), KKHVEVFTDGK (SEQ ID NO: 8), KDAAAAKNKMRHK (SEQ ID NO: 9), KSVVRHLGVTK (SEQ ID NO: 10), KFYQHVINGCK (SEQ ID NO: 11), KQVHQVQLTDK (SEQ ID NO: 12), KGDSCSSNCKH (SEQ ID NO: 13), HARLKGGLILK (SEQ ID NO: 14), KAMLLKKEPLLYVPIRLAGH (SEQ ID NO: 15), KHLVPLMHK (SEQ ID NO: 16), KYYAFLNKH (SEQ ID NO: 17), KAAVHKWK (SEQ ID NO: 18), KLNPSEDFIKH (SEQ ID NO: 19), KFCDHMVK (SEQ ID NO: 20), KPGHAMPSLFK (SEQ ID NO: 21), KNKMRHK (SEQ ID NO: 22), KRSHSPTK (SEQ ID NO: 23), KDAAAAKNKMRH (SEQ ID NO: 24), KMRHKRTSTK (SEQ ID NO: 25), HVERKDVPYPK (SEQ ID NO: 26), KGMQLLHTK (SEQ ID NO: 27), or KEGSSVTLKH (SEQ ID NO: 28), or at least one homologue of SEQ ID NO(s): 1-28. In a non-limiting embodiment, the isolated or synthesized protein fragment or peptide may consists essentially of at least one Replikin peptide sequence of SEQ ID NO(s): 1-28 or at least one homologue of SEQ ID NO(s): 1-28. In another non-limiting embodiment, the isolated or synthesized protein fragment or peptide may consist of at least one Replikin peptide sequence of SEQ ID NO(s): 1-28 or at least one homologue of SEQ ID NO(s): 1-28. Another non-limiting embodiment provides an isolated or synthesized peptide sequence comprising at least one functional fragment of at least one Replikin peptide sequence of SEQ ID NO(s): 1-28. Another non-limiting embodiment provides an isolated or synthesized peptide sequence comprising at least one functional fragment of at least one Replikin peptide sequence of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, 237, or 238. 
     A non-limiting embodiment of the first aspect of the invention provides an isolated or synthesized protein, protein fragment, polypeptide, or peptide comprising at least one Replikin peptide of an MERS CoV virus. A further embodiment of the first aspect of the invention provides an isolated or synthesized protein, protein fragment, polypeptide, or peptide comprising at least one peptide sequence that is at least 30%, 40%, 50%, 60%, 70%, 80%, 90% 95%, or 100%, homologous with at least one Replikin peptide sequence identified in a MERS CoV virus. In a non-limiting embodiment, the at least one sequence is one of SEQ ID NO(s): 1-28. 
     In a further non-limiting embodiment of the first aspect of the present invention, the isolated or synthesized protein, protein fragment, polypeptide, or peptide consists of 7 to about 50 amino acid residues comprising at least one peptide A, wherein said peptide A is at least 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, or 100%, homologous with at least one Replikin peptide sequence identified in an MERS CoV. In one non-limiting embodiment, said at least one Replikin peptide sequence identified in MERS CoV is at least one peptide sequence of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239, or SEQ ID NO(s): 1-28, SEQ ID NO(s): 1-9, SEQ ID NO(s): 5, 18, 22, and 23, or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. 
     In a further non-limiting embodiment of the first aspect of the present invention, the isolated or synthesized protein, protein fragment, polypeptide, or peptide consists essentially of a Replikin peptide identified in MERS CoV. In a further non-limiting embodiment, the Replikin peptide sequence identified in an MERS CoV is at least one peptide sequence of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. 
     In a non-limiting embodiment, the Replikin sequence is shared among isolates of MERS CoV isolated from camel and human. In a further non-limiting embodiment, the Replikin sequences shared among human and camel isolates are identical. In a further non-limiting embodiment, the lysine and histidine residues that make the Replikin sequences are identical among human and camel isolates while one or more other amino acid residues are not identical. In non-limiting embodiment, the Replikin sequences are conserved in camels and humans over one, two, three, or more years. 
     Another non-limiting embodiment of the first aspect of the invention provides a biosynthetic composition comprising the protein, protein fragment, polypeptide, or peptide of an aspect of the invention. In a further non-limiting embodiment, the biosynthetic composition consists essentially of a Replikin peptide of MERS CoV or consists of a Replikin peptide of MERS CoV. In a non-limiting embodiment, an isolated protein, protein fragment, polypeptide, or peptide is chemically synthesized by solid phase methods. 
     A second non-limiting aspect of the present invention provides an immunogenic and/or blocking composition comprising at least one protein, protein fragment, polypeptide, or peptide of any one of the above-listed proteins, protein fragments, polypeptides, or peptides including and not limited to comprising at least one Replikin peptide sequence identified in MERS CoV or at least one homologue of said at least one Replikin peptide identified in MERS CoV or at least one functional fragment of at least one Replikin peptide sequence identified in MERS CoV. In a non-limiting embodiment of the second aspect of the present invention, the immunogenic and/or blocking composition comprises at least one peptide sequence of SEQ ID NO(s): 1-28. In a further non-limiting embodiment, the immunogenic and/or blocking composition comprises at least one peptide consisting essentially of any one of SEQ ID NO(s): 1-28. In further non-limiting embodiment, the immunogenic and/or blocking composition comprises at least one peptide consisting of any one of SEQ ID NO(s): 1-28 or at least one functional fragment of any one of SEQ ID NO(s): 1-28. In a non-limiting embodiment, the Replikin sequence is shared among isolates isolated from camel and human. In a further non-limiting embodiment, the Replikin sequences are identical. In non-limiting embodiment, the Replikin sequences are conserved in camels and humans over one, two, three, or more years. 
     A third non-limiting aspect of the present invention provides a vaccine comprising at least one protein, protein fragment, polypeptide, or peptide comprising or consisting of a Replikin sequence or homologue thereof including any one of the above-listed proteins, protein fragments, polypeptides, or peptides. In a non-limiting embodiment, a Replikin sequence is shared among isolates isolated from camel and human. In a further non-limiting embodiment, the Replikin sequences are identical. In a further non-limiting embodiment, the lysine and histidine residues that make the Replikin sequences are identical among human and camel isolates while one or more other amino acid residues are not identical. In a non-limiting embodiment, the Replikin sequences are conserved in camels and humans over one, two, three, or more years. 
     In a non-limiting embodiment of the third aspect of the present invention, the vaccine comprises at least one peptide sequence of any one of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28, comprises at least one peptide sequence consisting essentially of any one of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28, and/or comprises at least one peptide sequence consisting of any one of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28, or at least one functional fragment of any one of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28, at least one functional fragment of a Replikin peptide sequence identified in MERS CoV, or at least one functional fragment of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. 
     In a further non-limiting embodiment of the third aspect of the present invention, the vaccine comprises a mixture of a plurality peptides of different sequences, wherein each of said peptides of different sequences is at least 80% homologous with at least one Replikin sequence identified in MERS CoV. In a non-limiting embodiment, the vaccine comprises a mixture of a plurality of peptides of different sequences, wherein at least one peptide of the mixture of a plurality of peptides of different sequences is at least 80% homologous with each of SEQ ID NO(s): 1-9, each of SEQ ID NO(s): 1-28, each of SEQ ID NO(s): 5, 18, 22, and 23, or each of SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. In a non-limiting embodiment, the vaccine comprises a mixture of a plurality of peptides of different sequences where at least one peptide of the mixture of a plurality of peptides of different sequences consists of each of SEQ ID NO(s): 1-9, each of SEQ ID NO(s): 1-28, each of SEQ ID NO(s): 5, 18, 22, and 23, or each of SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. In a non-limiting embodiment, a vaccine comprises at least one functional fragment of any one of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. In a non-limiting embodiment, a vaccine comprises a pharmaceutically-acceptable carrier and/or adjuvant. 
     In a non-limiting embodiment, a vaccine comprises a mixture of a plurality of peptide sequences of any of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28 and/or a mixture of a plurality of homologues of peptide sequences of any of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. In a further non-limiting embodiment, the vaccine comprises a mixture of a plurality of peptide sequences consisting essentially of any one or more of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. In a further non-limiting embodiment, the vaccine comprises a mixture of a plurality of peptide sequences consisting of any one or more of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. In a further non-limiting embodiment, the vaccine comprises a mixture of a plurality of peptides consisting of each of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28, or any combination of the listed sequences. 
     In another non-limiting embodiment of the third aspect of the invention, the vaccine comprises a mixture of Replikin peptides. In a non-limiting embodiment, the vaccine comprises an approximately equal molar mixture of the isolated or synthesized peptides of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. In a further non-limiting embodiment, the vaccine comprises approximately equal weight of the isolated or synthesized peptides of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. In a further non-limiting embodiment, the vaccine comprises a pharmaceutically-acceptable carrier and/or adjuvant. In a further non-limiting embodiment, the vaccine comprises peptides in sterile water. In a further non-limiting embodiment, the vaccine comprises freeze-dried peptides in sterile water. In a further non-limiting embodiment, the vaccine is for the treatment or prevention of MERS CoV infection. 
     A fourth non-limiting aspect of the invention provides an isolated or purified antibody, antibody fragment, or binding agent that specifically binds to at least a portion of a protein fragment or peptide comprising at least one peptide sequence that is at least 80% homologous with at least one Replikin peptide sequence identified in a MERS CoV. In a non-limiting embodiment, the isolate or purified antibody, antibody fragment, or binding agent specifically binds at least a portion of any one of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. In a non-limiting embodiment, the isolate or purified antibody, antibody fragment, or binding agent specifically binds at least a portion of any one of SEQ ID NO(s): 1-28. In a non-limiting embodiment, the isolate or purified antibody, antibody fragment, or binding agent specifically binds at least a portion of any one of SEQ ID NO(s): 1-9. In a non-limiting embodiment, the isolate or purified antibody, antibody fragment, or binding agent specifically binds at least a portion of any one of SEQ ID NO(s): 5, 18, 22, and 23 or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. 
     A fourth non-limiting aspect of the invention provides an isolated or purified antibody, antibody fragment, or binding agent that binds to at least a portion of an amino acid sequence of at least one protein, protein fragment, polypeptide, or peptide comprising a peptide sequence that is 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or more homologous with at least one Replikin peptide sequence identified in MERS COV. In a further embodiment, the at least one Replikin peptide sequence identified in MERS COV is at least one peptide sequence of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. 
     A fifth non-limiting aspect of the present invention provides a method of making a vaccine comprising: isolating or synthesizing a protein fragment or peptide comprising at least one peptide sequence that is at least 80% homologous with at least one Replikin peptide sequence identified in a MERS CoV as a component of a vaccine; and making the vaccine with the component. 
     A non-limiting embodiment comprises: selecting at least one isolated or synthesized protein, protein fragment, polypeptide, or peptide comprising at least one peptide sequence that is at least 30%, 40%, 50%, 60%, 70%, 80%, 90% or 95%, or 100% homologous with at least one Replikin peptide sequence identified in MERS CoV as a component of a vaccine; and making said vaccine. In a non-limiting embodiment, the method of making a vaccine comprises selecting at least one isolated or synthesized peptide of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28 as at least one component and making said vaccine with the at least one component. 
     In another non-limiting embodiment, the method of making a vaccine comprises selecting at least two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, sixteen, seventeen, eighteen, or up to 28 or more isolated or synthesized Replikin peptide sequences identified in MERS CoV and/or isolated or synthesized functional fragments of Replikin peptide sequences identified in MERS CoV and/or homologous of Replikin peptide sequences identified in MERS CoV. In a further embodiment, the isolated or synthesized Replikin peptide sequences, functional fragments of Replikin peptide sequences identified in MERS CoV, or homologues of Replikin peptide sequences identified in MERS CoV comprise at least one peptide sequence of SEQ ID NO(s): 1-28, at least one functional fragment of at least one peptide sequence of SEQ ID NO(s): 1-28, at least one functional fragment of at least one Replikin peptide sequence identified in MERS CoV, or at least one homologues of at least one Replikin peptide sequence identified in MERS CoV. In another non-limiting embodiment, the at least one isolated or synthesized protein, protein fragment, polypeptide, or peptide has the same amino acid sequence as at least one protein, protein fragment, polypeptide or peptide identified in a relatively lethal strain of MERS CoV up to seven days, one month, six months, one year, two years, or three years prior to making said vaccine. 
     A sixth non-limiting aspect of the present invention provides a method for preventing or treating MERS COV infection comprising administering at least one isolated or synthesized protein, protein fragment, polypeptide, or peptide comprising at least one peptide sequence to an animal or human, where the peptide sequence is at least 30%, 40%, 50%, 60%, 70%, 80%, 90% or 95%, or 100%, homologous with at least one Replikin peptide identified in MERS CoV. In a further non-limiting embodiment, the Replikin peptide sequence is at least one peptide sequence of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. In a further non-limiting embodiment, the Replikin peptide sequence is at least one peptide sequence of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. In a non-limiting embodiment, the at least one isolated or synthesized protein fragment, polypeptide, or peptide consists of at least one peptide sequence that is at least 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or more homologous with at least one of the peptide sequences of SEQ ID NO(s): 1-28. In another non-limiting embodiment, the at least one isolated or synthesized peptide of SEQ ID NO(s): 1-28 is administered to a human or other animal. In a further non-limiting embodiment the at least one Replikin peptide sequence is at least one peptide sequence of SEQ ID NO(s): 1-28. 
     A non-limiting embodiment of the sixth aspect provides use of at least one Replikin peptide sequence identified in MERS CoV, at least one homologue of at least one Replikin peptide sequence identified in MERS CoV, or at least one functional fragment of at least one Replikin peptide sequence identified in MERS CoV in the manufacture of a medicament for preventing or treating MERS CoV infection. In a non-limiting embodiment, the Replikin peptide sequence is any one of SEQ ID NO(s): 1-28, 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. A non-limiting embodiment provides use of a Replikin sequence, a homologue of a Replikin sequence, or a functional fragment of a Replikin sequence of MERS CoV for the prevention or treatment of MERS CoV. 
     A seventh non-limiting aspect of the present invention provides a method of predicting expansion or retraction of a population of MERS CoV comprising, respectively, identifying an increase in the percentage of isolates of MERS CoV having a Replikin concentration (number of Replikin sequences per 100 amino acid residues) in at least one portion of the genome greater than 4.0 between two time periods or identifying a decrease in the percentage of isolates of MERS CoV having a Replikin concentration (number of Replikin sequences per 100 amino acid residues) in at least one portion of the genome greater than 4.0 between two time periods. In a non-limiting embodiment, the more than two time periods are compared and the percentage of isolates in the more than two time periods shows a pattern of increase or decrease. In a non-limiting embodiment, the portion of the genome reflects encoding of an expressed protein. In a non-limiting embodiment, the portion of the genome is N, M, S, ORF4b, ORF1ab, ORF1a, ORF1b, NS3C, N3, or any other gene segment of the genome. 
     A non-limiting embodiment of the seventh aspect of the present invention provides a method of differentiating between relatively more lethal and relatively less lethal forms of MERS CoV. A non-limiting embodiment provides a method of identifying and/or diagnosing a relatively more lethal form of MERS CoV comprising determining the Replikin concentration of at least one portion of at least one protein of at least one isolate of MERS CoV or at least one portion of at least one gene that expresses at least one protein of the at least one isolate of MERS CoV and comparing the Replikin concentration of the at least one isolate of MERS CoV to a comparable Replikin concentration in at least one other isolate of MERS CoV. In a further non-limiting embodiment, the at least one portion of at least one protein comprises the entirety of at least one protein expressed in MERS CoV and the comparable Replikin concentration is the Replikin concentration of the entirety of the same protein expressed in MERS CoV from the at least one other isolate of MERS CoV. In a non-limiting embodiment, the Replikin concentration of the at least one isolate of MERS CoV is a mean of Replikin concentrations determined in a plurality of isolates of MERS CoV. In a further non-limiting embodiment, the Replikin concentration of the at least one other isolate of MERS CoV is a mean of Replikin concentrations determined in a plurality of other isolates of MERS CoV. In a further non-limiting embodiment, the plurality of isolates of MERS CoV is a collection of isolates isolated in a given year and the plurality of other isolates of MERS COV is a collection of isolates isolated in a different year. In a further non-limiting embodiment, the Replikin concentration of the more lethal isolate of MERS COV is 3.0 or greater, 4.0 or greater, or 5.0 or greater per 100 amino acid residues. In a further non-limiting embodiment, the Replikin concentration of the more lethal isolate of MERS COV is 4.0 or greater per 100 amino acid residues. In a further non-limiting embodiment, the Replikin concentration of the more lethal isolate of MERS COV is 4.6 per 100 amino acid residues or greater. In a further non-limiting embodiment, a vaccine is manufactured following the differentiation between relatively more lethal and relatively less lethal forms of MERS CoV. In a further non-limiting embodiment, the vaccine comprises at least one structure of the isolate of MERS CoV differentiated as relatively more lethal. In a further non-limiting embodiment, the vaccine comprises at least one Replikin peptide sequence identified in the isolate of MERS CoV differentiated as relatively more lethal. 
     In a further non-limiting embodiment of the seventh aspect of the present invention, the at least one portion of at least one gene expressing at least one protein is at least one portion of the ORF4b gene region. 
     In a further non-limiting embodiment of the seventh aspect of the present invention, the Replikin concentration of the at least one isolate of MERS CoV is greater than the Replikin concentration of the at least one other isolate of MERS CoV. In a further non-limiting embodiment the Replikin concentration is a mean Replikin concentration of a plurality of isolates with standard deviation from the mean and the standard deviation from the mean is greater than the standard deviation from the mean Replikin concentration of a plurality of other isolates. 
     Another non-limiting embodiment of the seventh aspect of the invention provides a method of determining an increased probability of an outbreak of MERS CoV within about one year following an increase in Replikin concentration in an isolate of MERS CoV comprising identifying an increase in the concentration of Replikin sequences in at least one first isolate of MERS CoV as compared to at least one other isolate of MERS CoV wherein said at least one first isolate is isolated at a later time period than said one other isolate and wherein said increase in the concentration of Replikin sequences signifies the increased probability of the outbreak of MERS CoV within about one year following the increase in the concentration of Replikin sequences. 
     In a non-limiting embodiment, a method of prediction comprises: (1) obtaining a plurality of isolates of MERS CoV wherein at least one of said isolates is isolated about six months to about 3 years later than at least one other of said isolates; (2) analyzing the amino acid sequence of at least one protein or protein fragment in each isolate of the plurality of isolates for the presence and concentration of Replikin sequences; (3) comparing the concentrations of Replikin sequences in the at least one protein or protein fragment in each isolate of the plurality of isolates one to another; (4) identifying an increase in the concentration of Replikin sequences in said plurality of isolates over at least one time period of about six months or greater; and (5) predicting an outbreak of MERS CoV within about one month to about three years following said identified increase in the concentration of Replikin sequences. In another embodiment of the invention, the outbreak of MERS CoV is predicted within about six months. In a further embodiment of the invention, the outbreak of MERS CoV is predicted within about one year to about three years. In a further non-limiting embodiment, the method of prediction further comprises processing at least one step of the method on a computer. 
     In a further non-limiting embodiment of the seventh aspect of the invention, the method of prediction further comprises comparison of the standard deviation from the mean of Replikin concentrations of isolates of MERS CoV from a given time period, such as a given month, a given year, or any other given time period. In a further non-limiting embodiment, the Replikin concentration is a mean Replikin concentration of a plurality of isolates with standard deviation from the mean and the standard deviation from the mean is greater than the standard deviation from the mean Replikin concentration of a plurality of other isolates. 
     A further non-limiting embodiment provides a computer readable medium having stored thereon instructions which, when executed, cause a processor to perform a method of predicting an expansion of a strain of MERS CoV or an increase in virulence, morbidity, and/or mortality of MERS CoV. In a further embodiment, the processor reports a prediction to a display, user, researcher, or other machine or person. In a further embodiment, the processor identifies to a display, user, researcher, or other machine or person, a portion of a pathogen predicted to be an expanding MERS CoV or predicted to increase in virulence, morbidity, and/or mortality, wherein said portion may be employed as a therapeutic or diagnostic compound. Said portion may be a Replikin peptide or plurality of Replikin peptides or any other structure or portion of said genome of said pathogen including a Replikin Peak Gene. 
     Another non-limiting embodiment of the seventh aspect of the invention provides a computer system, including a processor coupled to a network and a memory coupled to the processor, the memory containing a plurality of instructions to perform a method of predicting an increase in virulence, morbidity, or mortality as compared to at least one second malignancy. 
     Another non-limiting embodiment of the seventh aspect of the invention provides a machine-readable storage medium having stored thereon executable instructions that, when executed by a processor, cause the processor to provide sufficient data to a user, a display, or a printout such that said user or a user of said display or said printout may predict an increase in virulence, morbidity, or mortality of MERS CoV based on regression analysis. Another non-limiting embodiment provides a computer system, comprising: a processor coupled to a network; a memory coupled to the processor, the memory containing a plurality of instructions to perform the method of predicting an increase in virulence, morbidity, or mortality of MERS CoV based on the regression analysis. 
     A non-limiting embodiment provides an electromagnetic signal carrying computer-readable instructions for performing a method of predicting an outbreak of MERS CoV or the relative lethality or virulence of MERS CoV. In a non-limiting embodiment, the signal is non-transient. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  illustrates percent of publicly available genomic sequences of isolates of MERS CoV for given time periods analyzed as having Replikin sequences per 100 amino acid residues (Replikin Count) of greater than 4.0. The graph reflects analysis of the genomic or proteomic information publicly available for isolates at the PubMed website of the National Center for Biotechnology Information. Data are illustrated from April through June of 2012, May through June of 2012, September 2012, October 2012, and February through June 2013. The data show a rise in the percentage of MERS CoV isolates having a Replikin concentration (number of Replikin sequences per 100 amino acid residues) of greater than 4.0 from April through June 2012 to September 2012 followed by a decline in percentage of isolates of MERS having a Replikin concentration of greater than 4.0 from September 2012 through February through June 2013. The percentage of isolates of MERS CoV having a Replikin concentration of greater than 4.0 is around 16 percent in April through June 2012, rises to around 35 percent in September 2012, and falls back to around 17 percent in February through June of 2013. Observation of this fall in percent of isolates having Replikin concentration of greater than 4.0 predicts the MERS CoV outbreak of 2012 and 2013 will continue to diminish. 
         FIG. 2  illustrates an increase in Replikin concentration (Replikin sequences per 100 amino acid residues) in spike and nucleocapsid coronavirus proteins preceding the SARS coronavirus epidemic of 2003. The x-axis indicates the year and the y-axis indicates the Replikin concentration. The appearance of the SARS outbreak and the eight countries involved in the outbreak is shown by the conical shaded area. The solid black symbols represent the mean Replikin concentration for spike coronavirus proteins and the vertical black bars represent the standard deviation of the mean. Replikin concentration rose between 1995 and 2002, consistent with the SARS coronavirus outbreak, which emerged at the end of 2002 and persisted into 2003. The decline in Replikin concentration correctly signaled the end of the SARS outbreak and had already begun its return to pre-outbreak levels when the outbreak emerged. 
         FIG. 3  illustrates Replikin concentrations (Replikin sequences per 100 amino acid residues) for genomic or proteomic information publicly available for isolates of H1N1 at the PubMed website of the National Center for Biotechnology Information for hemagglutinin protein sequences (Infectivity Gene) and for pB1 protein sequences (Lethality Gene) from 2001 through April 2010. Gray bars illustrate mean Replikin concentration of hemagglutinin proteins with standard deviation bars above and black bars illustrate mean Replikin concentration of pB1 proteins with standard deviation bars above. The graph illustrates an increase in Replikin concentration (with a p value of less than 0.001) in Human H1N1 Influenza Virus Infectivity Gene from 2002 through April 2008, which prospectively predicted the global H1N1 influenza epidemic of 2009, followed by persistence in the elevation of Replikin concentration of the Infectivity Gene through April 2010. The persistence predicted that a clinical recurrence was likely, which was observed in December 2010. The data in  FIG. 3  illustrate mean Replikin concentration determinations annually from 2001 through 2008 and then mean Replikin concentration determinations every 2 to 3 days, then weekly, from Apr. 30, 2009. 
         FIG. 4  illustrates a high-level block diagram of a computer system incorporating a system and method for identifying Replikin patterns in amino acid sequences. 
         FIG. 5  illustrates a simple flow chart illustrating a general method for locating a Replikin pattern in a sequence of amino acids. 
         FIG. 6  illustrates a flow chart illustrating a generalized method for locating a plurality of Replikin-like patterns in a sequence of amino acids. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Definitions 
     A “protein fragment” as used in this specification is any fragment of an expressed whole protein, which is any portion of an expressed whole protein where a “portion” of a protein is less than an expressed whole protein. A protein fragment reflects an expressed whole protein with one or more amino acids removed from the amino acid sequence of the expressed whole protein. A protein fragment may also reflect an amino acid sequence that is at least 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or greater homologous with any portion of an expressed whole protein. A “polypeptide,” as used in this specification, is any portion of a protein fragment and is less than an expressed whole protein. 
     A “whole protein” or an “expressed whole protein” as used in this specification reflect a protein that is expressible from an intact gene of MERS CoV from a start codon to a stop codon. A whole protein or expressed whole protein may also reflect a whole protein or expressed whole protein that has been subject to cellular processing to create a protein that is capable of functioning within the virus replication system in a proper manner for virus replication. A protein fragment, polypeptide, or peptide “partially matches” the amino acid sequence of an expressed whole protein when the protein fragment, polypeptide, or peptide shares substantial homology with at least a portion of the expressed whole protein. 
     A “functional fragment” of a Replikin sequence as described herein is a fragment, variant, analog, or chemical derivative of a Replikin sequence that retains at least a portion of the immunological cross reactivity with an antibody specific for the Replikin sequence. A fragment of the Replikin sequence refers to any subset of the molecule. Variant peptides of the sequence may be made by direct chemical synthesis, for example, using methods well known in the art. An analog of a Replikin sequence to a non-natural protein or polypeptide is substantially similar to either the Replikin sequence of the protein or a fragment thereof. Chemical derivatives of a Replikin sequence contain additional chemical moieties. 
     As used herein, the term “preferentially binds” or “specifically binds” and related terms referencing the interaction of a binding molecule such as, for example, an antibody, and the structure to which it binds (antigen) means that the binding molecule preferentially recognizes the structure to which it binds even when present among other molecules (such as in a mixture of molecules). Specific or preferential binding of a binding molecule to a binding structure or an immunogenic portion of a binding structure is specific and preferential when the binding molecule binds to the structure or portion thereof and does not bind with the same level of affinity to other structures. Binding affinity may be determined by one of ordinary skill in the art using, for example, BIACORE, enzyme-linked immunosorbent assays, or radioimmuno assays. A binding molecule may cross-react with related antigens and preferably does not cross-react with affinity to unrelated antigens. Binding between a binding molecule and the structure to which it binds may be mediated by covalent or non-covalent attachment, or both. 
     As used herein a “vaccine” is any substance, compound, composition, mixture, or other therapeutic substance that, when administered to a human or animal via any method of administration known to the skilled artisan now or hereafter, produces an immune response, an antibody response, a blocking response, or a protective effect in the human or animal. 
     As used herein, a “Replikin sequence” is an amino acid sequence of 7 to 50 amino acid residues comprising (1) a first lysine residue located six to ten residues from a second lysine residue; (2) at least one histidine residue; and (3) at least 6% lysine residues, where the sequence is the shortest sequence comprising the first and second lysine residues of element (1) and the at least one histidine of element (2). A Replikin sequence may comprise more than two lysine residues and more than one histidine residue so long as at least two of the lysine residues and at least one histidine residue reflect the requirements of the definition of a Replikin sequence. A Replikin sequence may be targeted by the immune system within a peptide sequence that comprises more than the shortest sequence comprising the lysine and histidine residues required for a Replikin sequence. 
     The term “Replikin sequence” can also refer to a nucleic acid sequence encoding an amino acid sequence having 7 to about 50 amino acids comprising:
         (1) at least one lysine residue located six to ten amino acid residues from a second lysine residue;   (2) at least one histidine residue; and   (3) at least 6% lysine residues,
 
where the sequence is the shortest sequence encoding the first and second lysine residues of element (1) and the at least one histidine of element (2).
       

     In polypeptides, peptides, proteins, and protein fragments, the carboxyl group of one amino acid is attached to an amino group of another amino acid via a peptide bond forming a compound that is a chain of amino acid residues. 
     As used herein, a “synthesized” peptide may be synthesized by organic chemical methods and may be synthesized by biosynthetic methods. An “isolated” peptide may refer to a peptide that is, after purification, substantially free of cellular material or other contaminating proteins or peptides from the cell or tissue source from which the peptide is derived, or substantially free from chemical precursors or other chemicals when chemically synthesized by any method, or substantially free from contaminating peptides when synthesized by recombinant gene techniques. A protein or peptide that has been isolated in silico from nucleic acid or amino acid sequences that are available through public or private databases or sequence collections may be synthesized or isolated as compounds. An isolated peptide may be synthesized by biosynthetic or organic chemical methods. 
     Proteins, protein fragments, polypeptides, or peptides in this specification may be chemically synthesized by any method known to one of skill in the art now and hereafter. For example, isolated proteins, protein fragments, polypeptides, or peptides may be synthesized by solid phase synthesis. The production of these materials by chemical synthesis avoids the inclusion of (or the need to remove by purification) materials that are byproducts of other production methods such as recombinant expression or isolation from biological material. Such byproducts may include, for example, avian proteins associated with vaccines produced using birds&#39; eggs, bacterial proteins associated with recombinant production in bacteria, or proteins or contaminants associated with any recombinant activity such as with productions of proteins or other sequences in insect cells. 
     An “encoded” or “expressed” protein, protein sequence, protein fragment sequence, or peptide sequence is a sequence encoded by a nucleic acid sequence that encodes the amino acids of the protein or peptide sequence with any codon known to one of ordinary skill in the art now or hereafter. It should be noted that it is well known in the art that, due to redundancy in the genetic code, individual nucleotides can be readily exchanged in a codon and still result in an identical amino acid sequence. As will be understood by one of ordinary skill in the art, a method of identifying a Replikin amino acid sequence also encompasses a method of identifying a nucleic acid sequence that encodes a Replikin amino acid sequence wherein the Replikin amino acid sequence is encoded by the identified nucleic acid sequence. 
     “Homologous” or “homology” or “sequence identity” as used in this specification indicate that an amino acid sequence or nucleic acid sequence exhibits substantial structural equivalence with another sequence, namely, any Replikin peptide sequence (including SEQ ID NO(s): 1-28) identified in an isolate of MERS CoV or any nucleotide sequence encoding a Replikin peptide sequence in an isolate of MERS CoV (a redundancy in a coding sequence may be considered identical to a sequence encoding the same amino acid). To determine the percent identity or percent homology of an identified sequence, a sequence is aligned for optimal comparison purposes with any one of possible basis sequences. For purposes of this paragraph, a basis sequence is a Replikin sequence identified in an isolate of MERS CoV. Where gaps are necessary to provide optimal alignment, gaps may be introduced in the identified sequence or in the basis sequence. When a position in the identified sequence is occupied by the same amino acid residue or same nucleotide as the corresponding position in the basis sequence, the molecules are considered identical at that position (as used herein amino acid or nucleic acid “identity” is equivalent to amino acid or nucleic acid “homology”). To determine percent homology, the amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are compared between the identified (reference) sequence and the basis sequence. The total number of amino acid residues or nucleotides in the identified sequence that are identical with amino acid residues or nucleotides in the basis sequence is divided by the total number of residues or nucleotides in the basis sequence (if the number of residues or nucleotides in the basis sequence is greater than the total number of residues or nucleotides in the identified sequence) with gaps included or by the total number of amino acid residues or nucleotides in the identified sequence (if the number of residues or nucleotides in the identified sequence is greater than the total number of residues or nucleotides in the basis sequence) with gaps included. The final number is determined as a percentage. As such, the percent identity between the two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps (where a gap must be introduced for optimal alignment of the two sequences) and the length of each gap. Any structural or functional differences between sequences having sequence identity or homology will not affect the ability of the sequence to function as indicated in the desired application. 
     For example, SEQ ID NO: 6 (KHVEVFTDGK) is considered more than 90% homologous with SEQ ID NO: 8 (KKHVEVFTDGK). The more than 90% homology between SEQ ID NO: 6 and SEQ ID NO: 8 is determined as follows: SEQ ID NO: 6 is the identified sequence. SEQ ID NO: 8 is the basis sequence. Upon alignment, SEQ ID NO: 6 is identical to SEQ ID NO: 8 in all 11 residues of SEQ ID NO: 8 with the exception of the first lysine in SEQ ID NO: 8. To determine percent homology, then, the 10 aligned identical residues are divided by the total number of residues in SEQ ID NO: 8, namely 11 residues, giving 0.909 or greater than 90% homology. 
     In a further example, SEQ ID NO: 24 (KDAAAAKNKMRH) is more than 46% homologous with SEQ ID NO: 22 (KNKMRHK). SEQ ID NO: 24 is the basis sequence and has 12 residues. SEQ ID NO: 22 is the identified (or reference) sequence and has 7 residues. Six of the seven residues in SEQ ID NO: 22 are in the same position as six residues in SEQ ID NO: 24 when SEQ ID NO: 22 and SEQ ID NO: 24 are optimally aligned. A gap of one residue on the C-terminal end of SEQ ID NO: 24 must be added to encompass the lysine at the C-terminus of SEQ ID NO: 22. Including the gap of one residue on the C-terminal end, SEQ ID NO: 24 has a length of 13 residues. To determine percent homology, then, the 6 aligned identical residues are divided by the total number of residues in SEQ ID NO: 24 (with gap include), namely, 13 residues, giving 0.461 or more than 46% homology. 
     In another examples, SEQ ID NO: 22 (KNKMRHK) is more than 53% homologous with SEQ ID NO: 9 (KDAAAAKNKMRHK) because the seven residues of SEQ ID NO: 22 align exactly with seven residues of SEQ ID NO: 9 and SEQ ID NO: 9 has 13 total residues (giving greater than 53% homology). 
     Concerning gaps, the number of gaps in either the basis sequence or the identified sequence should be limited to the number of gaps allowable without significantly compromising the function of the identified sequence as compared to the basis sequence. In general, many gaps in the sequence of the basis peptide or in the sequence of the identified peptide are allowed based on homology as defined herein. Relatively more gaps are allowed if the lysines and histidines that create the definition of the Replikin peptide are identically shared between the basis peptide and the identified peptide. Relatively more gaps are also allowed if the lysines and histidines that create the definition of the Replikin peptide are shared at least in close position (for example within ten, nine, eight, seven, six, five, four, three, two, or one amino acid residue). If some of the lysine residues and histidine residues that create the definition of the Replikin peptide are not present in the identified peptide, fewer gaps may be allowed. Nevertheless, if the identified peptide functions similarly to the basis peptide, any number of gaps is allowed. In general, three or more gaps are allowed in the sequence of the basis peptide or in the sequence of the identified peptide within ten amino acid residues of the basis peptide if no lysines or histidines are present in the identified peptide. Two or more gaps or one or more gaps are also allowed. Nevertheless, if the identified sequence provides the same or a similar function to the basis sequence, more gaps are allowed up to the number of gaps that will provide a homology of 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, or more homology. Additionally, where the lysines and histidines of the Replikin definition are present in both the identified peptide and the basis peptide, there should be no limit on how many gaps are allowed. 
     The presence of lysines and histidines providing for the Replikin definition in an identified peptide requires significantly less homology because the lysines and the histidines of the Replikin definition provide for conservation of Replikin function. For example, in Table 8 and the description thereof in columns 62 and 63 in U.S. Pat. No. 7,442,761, a highly-mutable tat protein in HIV is described and analyzed. As may be seen from Table 8 in U.S. Pat. No. 7,442,761, in tat protein of HIV, which is essential for replication in the virus, lysines and histidines that are essential to maintaining the Replikin definition within a key Replikin peptide in the protein are observed to be 100% conserved, while substitutions in amino acid residues that are not essential to maintaining the Replikin definition are commonly substituted. The conservation of the key amino acids for maintaining the Replikin definition is understood to provide a specific survival function for HIV. The same phenomenon is seen in influenza. See U.S. Pat. No. 7,442,761, column 62, lines 42-45. 
     As used herein, “Replikin Count” or “Replikin concentration” refers to the number of Replikin sequences per 100 amino acids in a protein, protein fragment, virus, or organism. A higher Replikin concentration in a first strain of a virus or organism has been found to correlate with more rapid replication of the first virus or organism as compared to a second, earlier-arising or later-arising strain of the virus or organism having a lower Replikin concentration. Replikin concentration is determined by counting the number of Replikin sequences in a given sequence, wherein a Replikin sequence is a peptide of 7 to 50 amino acid residues comprising (1) a first lysine residue six to ten residues from a second lysine residue, (2) at least one histidine residue, (3) and 6% or more lysine residues where the Replikin sequence is the shortest sequence comprising the first and second lysine residues of element (1) and the at least one histidine residue of element (2). A Replikin sequence may comprise more than two lysine residues and more than one histidine residue so long as there is at least one lysine residue six to ten residues from a second lysine residue and at least one histidine residue. A Replikin sequence for the purpose of determining Replikin concentration as described in this paragraph may also be a nucleic acid that encodes a Replikin peptide sequence defined according to this paragraph. 
     Quantitative Real-Time Analysis of Replikin Sequences in MERS CoV 
     Quantitative Real-Time Replikins Analysis of the virus genome of lethal Middle Eastern Respiratory Syndrome Coronavirus (MERS CoV) demonstrates in  FIG. 1  that the percent of the virus&#39; genomic Replikins concentrations greater than 4.0 per 100 amino acids, an indication of virulence and infectivity, has dropped from its peak of 35% in 2012 to 17% on specimens collected up to June 2013. 
     The Figure summarizes all of the data listed in at the NCBI PubMed database. No data were available for large intervals of up to four months. Further, many sequences were published only up to 4 months after specimen collection. 
     The rapid quantitative decline in MERS CoV gene Replikin sequences reflected in  FIG. 1  resembles that previously found by Replikins Bioradar UK Ltd in the outbreak of SARS in 2002. See  FIG. 2 . SARS is a coronavirus related to MERS CoV. After an abrupt rise in gene Replikin Count in 2002, SARS Replikins began their immediate decline in concentration as the clinical outbreak occurred and quickly terminated in 2003. In another example, the occurrence of the H1N1 pandemic of 2009 was predicted one year in advance, its termination in 2009 (see  FIG. 3 ) and its recurrence in 2010, all were predicted by the Replikin Count. However, the course of MERS CoV appears to resemble that of its coronavirus SARS relative more than the course of the influenza virus H1N1 in the 2009 pandemic. Real-time quantitative gene analysis is changing the surveillance of emerging virus diseases and offers the possibility of pandemic prevention. 
     Because genomic Replikin changes can occur rapidly, and these correlate very closely with clinical outcomes, it is important that the genomic sequences be published as soon as possible after the specimens are collected. Ideal surveillance, attainable now, would have sequences published within a few days of specimen collection and Replikin analysis performed within an additional 24 hours. Testing completely synthetic Replikins vaccines based on this technology can be available quickly, in days to weeks. 
     Methods of Predicting Outbreaks of MERS CoV 
     One non-limiting aspect of the present invention provides a method of predicting expansion or retraction of a population of MERS CoV comprising, respectively, identifying an increase in the percentage of isolates of MERS CoV having a Replikin concentration (number of Replikin sequences per 100 amino acid residues) greater than 4.0 between two time periods or identifying a decrease in the percentage of isolates of MERS CoV having a Replikin concentration (number of Replikin sequences per 100 amino acid residues) greater than 4.0 between two time periods. In a non-limiting embodiment, the more than two time periods are compared and the percentage of isolates in the more than two time periods shows a pattern of increase or decrease. 
     In a further non-limiting embodiment, a plurality of isolates in a given time period may be analyzed for Replikin concentration. The percentage of isolates having a Replikin concentration of greater than 4.0 Replikin sequences per 100 amino acid residues in a first time period may be compared to the percentage of isolates having a Replikin concentration of greater than 4.0 Replikin sequences per 100 amino acid residue in a second time period. If the later timer period has a higher percentage of isolates having a Replikin concentration of greater than 4.0 then it is predicted that the MERS population will expand and an outbreak is more likely. If the later time period has a lower percentage of isolates having a Replikin concentration of greater than 4.0 then it is predicted that the MERS populations will retract and an outbreak is expected to decrease in severity. 
     For example,  FIG. 1  provides data demonstrating a marked increase in percent of isolates having a Replikin concentration of greater than 4.0 between April and September 2012 (predicting an outbreak) and a marked decrease in percent of isolates having a Replikin concentration of greater than 4.0 between September 2012 and June 2013 (predicting a retraction in the population). 
     One non-limiting aspect of the present invention provides a method of determining an increased probability of an outbreak of MERS CoV within about six months to about three years following an increase in Replikin concentration in an isolate of MERS CoV comprising identifying an increase in the concentration of Replikin sequences in at least one first isolate of MERS CoV as compared to at least one other isolate of MERS CoV wherein said at least one first isolate is isolated later than said at least one other isolate is isolated, and wherein said increase in the concentration of Replikin sequences signifies the increased probability of the outbreak of MERS CoV within about six months to about three years following the increase in the concentration of Replikin sequences. In a non-limiting embodiment, the first isolate of MERS CoV is isolated at least about six months later than the at least one other isolate. 
     In a non-limiting embodiment, a method of prediction comprises: (1) obtaining a plurality of isolates of MERS CoV, wherein at least one of said isolates is isolated later (less than six months later or about six months to about 3 years later) than at least one other of said isolates; (2) analyzing the amino acid sequence of at least one protein or protein fragment in each isolate of the plurality of isolates for the presence and concentration of Replikin sequences; (3) comparing the concentrations of Replikin sequences in the at least one protein or protein fragment in each isolate of the plurality of isolates one to another; (4) identifying an increase in the concentration of Replikin sequences in said plurality of isolates over at least one time period (said period may be one day, one week, one month, or six months or greater); and (5) predicting an outbreak of MERS CoV following said identified increase in the concentration of Replikin sequences. In one embodiment of the invention, the outbreak of MERS CoV is predicted within about six months to about five years. In a further embodiment of the invention, the outbreak of MERS CoV is predicted within about one year to about three years. 
     In a further non-limiting embodiment of the aspect of the invention, the method of prediction further comprises comparison of the standard deviation of the mean of Replikin concentrations of isolates of MERS CoV from a given time period, such as a given month, a given year, or any other given time period. In a further non-limiting embodiment the Replikin concentration is a mean Replikin concentration of a plurality of isolates with standard deviation from the mean and the standard deviation from the mean is greater than the standard deviation from the mean Replikin concentration of a plurality of other isolates. 
     Computer Methods for Determining Expansion, Retraction, and Outbreaks of MERS CoV 
     A prediction of expansion or retraction of a MERS CoV population may be performed by a processor. A prediction may be output to a user or display. Likewise, a particular Replikin peptide or Replikin Peak Gene within an isolate or population of isolates of MERS CoV predicted to be expanding or retracting in replication or lethality may be output to a user or display. A machine-readable storage medium may contain executable instructions that, when executed by a processor, cause the processor to provide sufficient data to a user, a printout, or a display such that the user or a user of the printout or display may predict expansion or retraction of population of MERS CoV. A process for prediction may comprise: comparing a Replikin Count of at least one first isolate of MERS CoV with a Replikin Count of at least one second isolate of MERS CoV; and predicting the population of the first isolate to be expanding if the Replikin Count of the first isolate is greater than the Replikin Count of the second isolate. In another embodiment, predicting the population of the first isolate to be expanding if the Replikin Count of the first isolate is greater than four Replikin sequences per 100 amino acid residues. 
     A computer system may include a processor coupled to a network, and a memory coupled to a processor, wherein the memory contains a plurality of instruction to perform the methods of prediction discussed herein. 
     A user of outputted data from a processor, storage medium, machine-readable medium, signal, or computer system may include any person or any machine that records or analyzes the outputted data. A display or printout may include any mechanism by which data is outputted so that any person or any machine may record or analyze the outputted data, including a printed document, a visual impulse, an aural impulse, a signal, or any other perceivable impulse, a computer monitor, a set of numbers, or any other display or printout of data including a digital recording medium. 
     Concerning a non-limiting computer system that may implement methods disclosed in the application,  FIG. 4  is a high-level block diagram of a computer system incorporating a system and method for identifying Replikin patterns in amino acid sequences. As shown in  FIG. 4 , computer workstation  410  may be a computer having a processor and a memory configured to permit a researcher to search protein databases and to scan protein descriptions for selected amino acid patterns. To accomplish these functions, computer workstation  410  may include protein and amino acid research system  430 , which may receive instructions from a user/researcher to conduct protein searching and amino acid scanning operations. Protein and amino acid research system  430  may further include amino acid sequence scanner  440  that scans and searches retrieved protein and amino acid sequences for specific patterns of amino acids, including Replikin patterns. Protein and amino acid research system  430  may communicate with network interface  420  to obtain protein sequences and amino acid sequences from resources on network  460 , which may include the Internet. Alternatively, protein and amino acid research system  430  may obtain protein sequences and amino acid sequences from a local protein database  450 . In addition, protein and amino acid research system  430  may obtain protein sequences and amino acid sequences directly from other input means, such as keyboard input. Protein and amino acid research system  430  may also communicate with network interface  420  to transmit results to other computers on network  460 . 
       FIG. 5  is a simple flow chart illustrating a general method for locating a Replikin pattern in a sequence of amino acids. The method  500  may begin after a sequence of amino acids has been obtained. Typically, the sequence of amino acids may be represented by three alphabetic characters. However, other encodings are envisioned by the present invention as well. Referring to  FIG. 5 , once a sequence of amino acids has been obtained, the sequence is searched for a Replikin pattern ( 510 ), which comprises a subsequence (or string) of amino acids that includes the following characteristics:
         (1) the string contains from 7 to about 50 amino acids;   (2) the string contains at least one lysine residue located 6 to 10 positions from a second lysine residue;   (3) the string contains at least one histidine residue; and   (4) the string contains at least 6% lysine residues.       

     Once a string of amino acids is found to match the Replikin pattern, the string may be identified or marked (520) accordingly. 
     A given sequence of amino acids may contain many subsequences or strings that match the Replikin pattern. Additionally, Replikin patterns may overlap each other. Thus, to locate and identify all possible Replikin patterns in a sequence of amino acids, method  500  may be invoked iteratively for each subsequence of amino acids contained within the original sequence of amino acids. 
     When method  500  is invoked iteratively to identify and locate all possible Replikin patterns in an amino acid sequence, the number of resulting Replikin patterns may be counted. A Replikin count may be reported as an absolute number. Additionally, a ratio of the number of Replikins per N amino acids in the sequence may be calculated. For example, it may be determined that a given protein contains a ratio of 6 Replikin sequences for every 100 amino acid residues. Replikin ratios have been shown by laboratory experiment and by epidemiological evidence to correlate directly to the rate that a given virus replicates. Rapid replication in pathogens is an indication of disease. For example, the presence of relatively high ratios of Replikin patterns has been correlated to epidemics of influenza. Similarly, an increase in the count of Replikin patterns observed in a protein over time indicates a future disease caused by the organism from which the protein was obtained. 
       FIG. 6  is a flow chart illustrating a generalized method  600  for locating a plurality of Replikin patterns in a given sequence of amino acids. The method  600  begins by locating a first lysine residue in the given sequence ( 610 ). Then, the method  600  may determine whether a second lysine residue resides within kmin to kmax positions of the first lysine residue ( 620 ). As indicated in  FIG. 6 , kmin and kmax define the limits on the distance between the first and second lysine residues. For a typical Replikin pattern, kmin will equal 6 and kmax will equal 10. However, these values may be varied by a researcher interested in discovering other similar patterns. 
     Once method  600  has identified two lysine residues that are close enough to each other ( 620 ), the method  600  may examine every histidine residue that resides within rmax positions of both the first and second lysine residues ( 630 ). When method  600  is employed to identify and locate typical Replikin patterns, rmax will be set to equal 50. For every histidine residue that resides within rmax positions of the two lysine residues identified in steps ( 610 ) and ( 620 ), method  600  will construct the shortest string of amino acid residues that includes the first lysine residue, the second lysine residue, and the identified histidine residue ( 640 ). Then, method  600  will determine whether the length of that shortest string is within the desired range—that is, whether it contains at least rmin amino acid residues and no more than rmax amino acid residues ( 650 ). Finally, if the identified string of amino acids also contains at least kpercent of lysine residues ( 660 ), the string will be identified as matching the desired Replikin pattern ( 670 ). 
     Still referring to  FIG. 6 , it is apparent that method  600  may identify several Replikin patterns from a single given amino acid sequence. This may happen because method  600  may examine more than one histidine residue that resides within rmax positions of the two identified lysine residues. Each identified histidine residue may, in combination with the two lysine residues, match the desired Replikin-like pattern. 
     Methods of Determining Relative Lethality of Isolates of MERS CoV 
     One non-limiting aspect of the present invention provides methods of predicting expansion or retraction of outbreaks of MERS CoV. Compounds for diagnostic, therapeutic, and/or preventive purposes in MERS CoV and therapies for the prevention and treatment of MERS CoV are provided based on the disclosed methods of prediction. 
     Replikin Peptide Sequences Available for Therapies in MERS CoV Across Strains and in Different Countries 
     An aspect of the present invention provides compounds for diagnostic, therapeutic, and/or preventive purposes in MERS CoV, methods of differentiating relative lethality between one or more isolates of MERS COV, and methods of designing therapies against MERS CoV based on compounds of the invention and differentiation of lethality among isolates of MERS CoV. 
     Compounds of one aspect of the invention comprise Replikin peptides and homologues of Replikin peptides identified in and isolated from different strains of MERS CoV and include Replikin peptides conserved over time in the same and different strains of MERS CoV. These Replikin peptides are useful when comprised in immunogenic and blocking compounds to provide a protective effect against MERS CoV infection including antagonism of the lethality of strains of MERS CoV. Replikin peptides that are conserved within strains of MERS CoV over time or across different strains of MERS CoV at conserved positions in the different strains of MERS CoV provide the ordinary skilled artisan with an expectation that the functionality of these peptides share commonality among various strains of MERS CoV and among various isolates of the same strain of MERS CoV at different times. 
     Four peptides provided in an aspect of this invention were identified as conserved in isolates of MERS CoV. The four peptides are combined in a vaccine for administration against challenge by MERS CoV. The vaccine is designed to generate therapeutic blocking response and an immune response that antagonizes infectivity, replication, and transmission of MERS CoV. Any virus that is not blocked on entry will be blocked intracellularly. 
     The four peptides were surprisingly identified as conserved in MERS CoV across regions and time. The ORF4b gene area of MERS CoV has been shown to be a Replikin Peak Gene area of the virus and Replikin Peak Gene areas of other viruses and pathogens have been demonstrated to be associated with lethality. See, e.g., WO 2008/143717, FIGS. 10-13, 16, 17, and 19. 
     The peptides and their homologues described herein are, among other things, antigenic, common to various strains of MERS CoV in both position and function, and conserved in various strains of MERS CoV over time. One of ordinary skill in the art expects the Replikin peptides and their homologues described herein to be useful in immunogenic and blocking compounds for therapies against MERS CoV within strains, across strains, and across time. 
     Shared and Conserved Replikin Peptide Sequences and their Homologues 
     Replikin sequences and their homologues provided by an aspect of the invention may be identified in strains of MERS CoV including any strain of MERS CoV known now or identified or known hereafter. Compounds of the invention may be conserved within strains of MERS CoV, across types within strains of MERS CoV, and across strains of MERS CoV. Compounds of the invention may be conserved in humans and camels and may be preserved over one, two, or more years in these hosts. In a non-limiting embodiment, the Replikin sequences are shared among isolates isolated from camels and humans. In a non-limiting embodiment, the Replikin sequences are identical isolates isolated from camels and humans. In a non-limiting embodiment the Replikin sequences are identical and shared among camels and humans and are conserved among camels and humans. The compounds, because they are Replikin sequences, related to Replikin sequences, derived from Replikin sequences, identified as comprising Replikin sequences, or designed to comprise Replikin sequences, are related to rapid replication, virulence, and lethality in MERS CoV and comprise necessary structure for antigenicity. These characteristics of Replikin sequences have been previously established in other viruses and organisms (see, e.g., U.S. Pat. No. 7,894,999, U.S. Pat. No. 7,758,863 and WO 2008/143717) but have not previously been disclosed in MERS CoV; and the surprisingly effective utility of the Replikin sequence in predictions and therapies in MERS CoV is established herein. Compounds of the invention, including conserved Replikin peptides, are useful as immunogenic compounds to stimulate the immune system of a subject to produce an immune response, which may include production of antibodies or other binding molecules. Compounds of the invention are also useful in therapies such as vaccines. Compounds of the invention are likewise useful in producing antibodies, antibody fragments, or other binding or antagonizing agents, which may be used, among other things, for diagnostic and therapeutic purposes, including passive immunity. Conservation between camel reservoirs and human hosts allows for targeting of the Replikin structures in both hosts. 
     The immunogenic compounds, antibodies (and other binding or antagonizing agents) and vaccines of the invention are useful against any strain of MERS CoV and are likewise useful against other coronaviruses. The compounds of the invention are also useful for diagnostic purposes, including identifying rapidly replicating, virulent, or lethal strains of virus. 
     Information on the conservation of homologous sequences across various strains of MERS CoV and in different regions provides sequences that offer immunogenic compounds for antagonism of all strains comprising these homologues across all regions having strains comprising these homologues. As a result, a vaccine is provided herein that offers cross-strain protection for a variety of strains of MERS CoV. 
     Replikin peptides in general are seen to be conserved across strains of MERS CoV. The key amino acid residues that provide for the Replikin sequence structure are the lysine and histidine residues wherein a Replikin sequence has at least one lysine on one terminus and at least one lysine or one histidine on the other terminus, at least one lysine that is six to ten residues from at least one other lysine, at least one histidine, and at least six percent lysines in total between the terminal lysine and the terminal lysine or histidine. 
     As may be seen in FIG. 10 of WO 2005/104754, when conserved homologous Replikin sequences are aligned one on top of the other over time, it is most apparent that fixed and conserved portions of the structure of Replikin sequences align in a series of posts or girders that illustrate, like the structure of a building, how key conserved amino acids provide constancy for the survival of a virus such as MERS CoV over time as it mutates to avoid immune recognition in its prospective host but maintains key functional genetic structures that provide for continued replication of the virus. These key functional genetic structures provide targets antagonized by Replikin-based therapies. 
     Compounds and Compositions Comprising Peptides Homologous to MERS COV Replikin Peptides 
     One aspect of the present invention provides a protein, a protein fragment, a polypeptide, or a peptide that comprises at least one peptide A homologous with at least one Replikin peptide identified in an isolate of MERS CoV. The Replikin peptide may be any Replikin peptide identified in an isolate of MERS COV. The Replikin peptide may further be a Replikin peptide identified as conserved across strains or across regions in isolates of MERS COV or any homologue of a Replikin peptide identified as conserved across strains or across regions in isolates of MERS COV. For example, the Replikin peptide may be any one of SEQ ID NO(s): 1-28 or any homologue of any one of SEQ ID NO(s): 1-28. 
     Peptide A of the protein, protein fragment, polypeptide, or peptide may be 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or more homologous or 100% homologous with a Replikin peptide, including any of the peptides of SEQ ID NO(s): 1-28. A protein fragment or peptide may likewise be a peptide that consists of a peptide A that is homologous with a Replikin peptide of MERS COV, including any of SEQ ID NO(s): 1-28. A peptide consisting essentially of or consisting of a Replikin peptide of MERS COV, including any one of SEQ ID NO(s): 1-28, is also provided. 
     The amino acid sequence of the provided isolated or synthesized protein, protein fragment, polypeptide, or peptide may partially match an amino acid sequence of an expressed whole protein. At least one, five, ten, twenty, thirty, forty, fifty, one hundred, two hundred, three hundred, four hundred, five hundred, five hundred and fifty or more amino acid residues of the amino acid sequence of the expressed whole protein may not be present in the protein, protein fragment, polypeptide, or peptide. The amino acid sequence of an isolated or synthesized protein fragment, polypeptide, or peptide may also partially match the amino acid sequence of an expressed whole protein where at least one, ten, twenty, thirty, forty, fifty, sixty, seventy, eighty, ninety, one hundred, one hundred fifty, two hundred, two hundred fifty, three hundred, three hundred fifty, four hundred, four hundred fifty, five hundred, five hundred fifty or more amino acid residues of at least one terminus of the amino acid sequence of the expressed whole protein is (are) not present at at least one terminus of said protein fragment, polypeptide, or peptide. Any additional number of amino acids may be situated on one or the other terminus or on both termini of the protein fragment, polypeptide, or peptide. 
     Because a Replikin peptide, such as SEQ ID NO(s): 1-28, is associated with rapid replication, infectivity, and/or lethality, in functional proteins in MERS CoV and because a Replikin peptide such as any one of SEQ ID NO(s): 1-28 is antigenic, inclusion of any Replikin peptide in a protein, protein fragment, polypeptide, or peptide does not negate the functional nature of the Replikin peptide. As such, antagonism of at least one Replikin peptide, including at least one of SEQ ID NO(s): 1-28 or a homologue of SEQ ID NO(s): 1-28 (with homology of 30% or greater) within a protein, protein fragment, polypeptide, or peptide would be expected to antagonize the replication, infectivity, and/or lethality of the protein fragment, polypeptide, or peptide. 
     A provided peptide may further be a peptide B of 7 to about 50 amino acid residues where peptide B contains a peptide A that is 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or more homologous or 100% homologous with any Replikin peptide, including one of SEQ ID NO(s): 1-28. A non-limiting peptide may further be a peptide A that is a Replikin peptide. 
     An isolated or synthesized protein, protein fragment, polypeptide, or peptide may consist of a peptide that is 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, or 100% homologous with at least one of SEQ ID NO(s): 1-28 where the length of the peptide is no more than one, five, ten, twenty, thirty, forty, or fifty amino acid residues longer than a Replikin peptide of MERS COV including the sequence of SEQ ID NO(s): 1-28 with which it is homologous. An isolated or synthesized protein fragment, polypeptide, or peptide may likewise be no more than one, two, three, four, five, six, seven, eight, nine, or ten amino acid residues longer than the Replikin sequence with which it is homologous (including, for example, SEQ ID NO(s): 1-28). 
     Another non-limiting embodiment provides a biosynthetic composition of the invention. The biosynthetic composition may comprise the isolated or synthesized protein, protein fragment, polypeptide, or peptide of an aspect of the invention disclosed herein. The biosynthetic composition may further consist essentially of a Replikin peptide of a MERS CoV. In a further embodiment, the Replikin peptide of a MERS CoV is at least one peptide having a sequence of SEQ ID NO(s): 1-28. A further non-limiting embodiment provides a biosynthetic composition consisting of a Replikin peptide of MERS CoV. In a non-limiting embodiment, the isolated protein fragment, polypeptide, or peptide of an aspect of the invention is chemically synthesized by solid phase methods. 
     An isolated or synthesized polypeptide or peptide may comprise a peptide A that has about the same number of amino acid residues as a peptide B, where peptide B is one of the peptides of SEQ ID NO: 1-28 and where the lysine residues and histidine residues in peptide A are conserved as compared to the lysine residues and histidine residues in peptide B. An isolated or synthesized polypeptide or peptide comprising peptide A may have up to 100 additional amino acid residues as compared to peptide B. Some or all of the up to 100 additional amino acid residues may be positioned toward the amino-terminus and/or carboxy-terminus of the lysine or histidine termini of peptide A. Some of the additional amino acid residues may be positioned within the lysine or histidine termini of peptide A so long as a level of homology is maintained between peptide A and peptide B that retains at least some of the functionality of the Replikin peptide of peptide B. Functionality may include, but is not limited to, antigenicity, rate of replication, antagonizability of a protein containing said peptide A or said peptide B, binding capacity of binding agents to peptides A or B, etc. 
     An isolated or synthesized polypeptide or peptide may also comprise up to about 90, about 80, about 70, about 60, about 50, about 40, about 30, about 20, about 10, about 5, about 4, about 3, about 2, or about 1 additional amino acid residues. The residues may be entirely outside of the Replikin structure or entirely within the Replikin structure or partially within and partially outside the Replikin structure. A level of homology should be maintained between peptides B and A when additional residues are present or are added. Residues outside of the Replikin structure are those residues on the amino-terminus or carboxy-terminus of the polypeptide or peptide as compared to the lysine or histidine termini of peptide A. Residues within the Replikin structure are those residues that are between the lysine or histidine termini of peptide A. An isolated or synthesized polypeptide or peptide may also consist of peptide A and peptide A may consist of peptide B. 
     The at least one isolated or synthesized protein, protein fragment, or peptide may also comprise at least one peptide A and at least one peptide C where peptide A is at least 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, or 100% homologous with at least one Replikin peptide of MERS CoV, which may include a peptide of SEQ ID NO(s): 1-28, and where peptide C is at least 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, or 100% homologous with at least one other Replikin peptide of MERS CoV, which also may include a peptide of SEQ ID NO(s): 1-28. Peptide C may be homologous with a different Replikin peptide than the peptide that peptide A is homologous with. The at least one isolated or synthesized protein, protein fragment, or peptide may comprise three or more peptides homologous with at least three different Replikin peptides. 
     All of the above-discussed proteins, protein fragments, polypeptides, and peptides comprise the functional unit of a homologue of a Replikin peptide present in or isolated from an MERS CoV. The Replikin peptide may be any one of SEQ ID NO(s): 1-28 or any one of SEQ ID NO(s): 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. Antagonism of any of the homologues of a Replikin peptide will antagonize replication in MERS CoV. As a result, the proteins, protein fragments, polypeptides, and peptides are useful as immunogenic compounds, therapeutic compounds, vaccines, and for other therapies directed to antagonizing the replication and/or lethality of a strain of MERS CoV. When comprised in a vaccine, disclosed proteins, protein fragments, polypeptides, and peptides are expected to be capable of limiting the excretion or shedding of MERS CoV such that the virus is limited in its spread from host to host or from host to reservoir to host, etc. As such, disclosed compounds are effective at limiting sources of MERS CoV infection. Likewise, any binding agent that binds one of the proteins, protein fragments, polypeptides, and peptides discussed above will antagonize the replication and/or lethality of a strain of MERS CoV and limit sources of MERS COV infection such as transmission from host to host or from host to reservoir to host. 
     Immunogenic Compositions Comprising Peptides Homologous to MERS CoV Replikin Peptides 
     A protein, protein fragment, or peptide comprising a Replikin peptide present or identified in an isolate of MERS CoV may be comprised in an immunogenic compound. The proteins, protein fragment, polypeptides, and peptides provided by an aspect of the invention comprise at least a portion that is homologous with a Replikin peptide or homologous with one of the MERS CoV Replikin peptides of SEQ ID NO(s): 1-28. These homologues are expected by one of ordinary skill in the art to stimulate the immune system of a subject upon sufficient exposure to produce antibodies against at least the homologous portion of the protein, protein fragment, polypeptide, or peptide and/or to produce a protective effect against MERS CoV. One of ordinary skill in the art would expect that antibodies or other binding agents arrayed against a protein or protein fragment comprising one of the antigenic homologues disclosed herein would be antagonistic to the protein or protein fragment. 
     One of ordinary skill would also expect an antagonist of one of these homologues to antagonize any MERS CoV that comprises a homologue of a Replikin peptide. Because homologues of SEQ ID NO(s): 1-28 have been shown to be conserved across strains and time, one of ordinary skill would expect antagonism of such homologues to result in antagonism of MERS CoV replication. One of ordinary skill would further expect particular antagonism of the lethality mechanisms of MERS CoV when an immune system is stimulated against a homologue of any one or more of SEQ ID NO(s): 1-28. 
     As a result, one aspect of the present invention is a method of stimulating the immune system of a subject with at least one compound comprising at least one Replikin sequence identified in MERS CoV or at least one isolated or synthesized homologue or functional fragment of at least one Replikin sequence identified in MERS CoV. The at least one Replikin sequence of the compound reflects an immunogenic target against which the immune system of the subject responds. Because at least a functional portion of the immunogenic structure of the target is maintained in a functional fragment of the at least one Replikin sequence, a functional fragment of the Replikin sequence is likewise a target against which the immune system of the subject responds. The compound may comprise a protein comprising the at least one Replikin sequence or functional fragment thereof, a protein fragment, a polypeptide, or a peptide comprising the at least one Replikin sequence or functional fragment thereof. The compound may comprise more than one protein, protein fragment, polypeptide or peptide. The compound may further be a composition of a plurality of synthesized or isolated Replikin sequences. 
     Vaccines Comprising Peptides Homologous to MERS COV Replikin Peptides 
     An immunogenic compound provided as an aspect of the invention may be used as a component of a non-limiting vaccine against any strain of MERS CoV. A vaccine comprising one or more homologues of a Replikin peptide of MERS CoV may be used against MERS CoV. The vaccine may comprise one or more homologues of SEQ ID NO(s): 1-28 or 30-172, 174-190, 192-209, 211-222, 224-235, and 237-239. Likewise, a vaccine comprising one or more homologues of a Replikin peptide may be used against MERS CoV and may antagonize the replication and/or lethality of an MERS CoV infection. Further, mixtures of homologues of SEQ ID NO(s): 1-28 are provided as vaccines to antagonize the replication and/or lethality of an MERS CoV infection. Such vaccines are useful for antagonizing replication, lethality, and excretion or spread of MERS CoV. 
     One vaccine may comprise at least one protein, protein fragment, polypeptide, or peptide of any one or more of the proteins, protein fragments, polypeptides, or peptides of an aspect of the invention. The vaccine may further comprise at least one Replikin peptide of MERS CoV. One such Replikin peptide may be any one of SEQ ID NO(s): 1-28. A vaccine may comprise at least one peptide consisting essentially of any one of SEQ ID NO(s): 1-28 or at least one peptide consisting of any one of SEQ ID NO(s): 1-28. A vaccine may comprise a mixture of Replikin peptides of MERS CoV. The vaccine may comprise a plurality of peptides such as two, three, or four peptides of SEQ ID NO(s): 1-28. The peptide may consist essentially of any one or more SEQ ID NO(s): 1-28 or may consist of any one or more of SEQ ID NO(s): 1-28. 
     In another non-limiting embodiment, the vaccine comprises a mixture of peptides, wherein the mixture comprises isolated or synthesized peptides of SEQ ID NO(s): 1-9, SEQ ID NO(s):1-28, SEQ ID NO(s): 5, 18, 22, and 23, or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. In a non-limiting embodiment, the vaccine comprises an approximately equal molar mixture of the isolated or synthesized peptides of SEQ ID NO(s): 1-9, SEQ ID NO(s):1-28, SEQ ID NO(s): 5, 18, 22, and 23, or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. In a further non-limiting embodiment, the vaccine comprises approximately equal weight of the isolated or synthesized peptides of SEQ ID NO(s): 1-9, SEQ ID NO(s):1-28, SEQ ID NO(s): 5, 18, 22, and 23, or SEQ ID NO(s): 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. 
     A vaccine may comprise a plurality of the shortest Replikin peptides from any region of the genome of MERS CoV including the ORF  4   b  gene area. A vaccine may comprise the shortest Replikin peptides from a gene area identified in a MERS CoV isolate or a plurality of MERS CoV isolates predicted to have a greater lethality than at least one other isolate of MERS CoV. 
     A vaccine may further comprise a plurality of the longest Replikin peptides from any gene area of the virus including the ORF4b gene area identified in an MERS CoV isolate or a plurality of MERS CoV isolates. A vaccine may also comprise a mixture of the shortest and longest Replikin peptides identified in the ORF4b gene area or any other gene area. 
     A vaccine may be formulated with a pharmaceutically acceptable excipient, carrier, or adjuvant. One pharmaceutically acceptable carrier or excipient is sterile water. A vaccine may comprise freeze-dried Replikin peptides in sterile water. Excipients, carriers, or adjuvants may include, but are not limited to, excipients, carriers and adjuvants known to those of skill in the art now or hereafter. 
     A non-limiting acceptable carrier or adjuvant may include sterile water, oil and water emulsion, or keyhole limpet hemocyanin, A non-limiting carrier and/or adjuvant may include a sterile diluent such as water (for dermal, nasal, or ocular application, spraying, or injection), saline solution, fixed oils, polyethylene glycols, glycerin, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as EDTA; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. Preparations may be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic. 
     Pharmaceutical compositions suitable for use typically include sterile aqueous solutions (water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. For intravenous administration, suitable carriers include physiological saline, bacteriostatic water, Cremophor EL™ (BASF, Parsippany, N.J.) or phosphate buffered saline (PBS). In general, a relevant carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyetheylene glycol, and the like), and suitable mixtures thereof 
     In a further non-limiting embodiment, one adjuvant is a UTOPE. A UTOPE may be covalently attached to an isolated or synthesized peptide at the C-terminus, the N-terminus, or both termini. A UTOPE is a peptide sequence of 6 to 10 residues comprising one histidine residue with all other residues being lysine residues. A UTOPE may be included in a vaccine for the treatment or prevention of MERS CoV infection. 
     Peptides may be synthesized using L- or D-amino acids and may be synthesized with chemical moieties to discourage breaking of peptide bonds in biological systems. 
     A composition of the invention may be formulated for delivery by any available route including, but not limited to parenteral (e.g., intravenous), intradermal, subcutaneous, oral, nasal, bronchial, ophthalmic, transdermal (topical), transmucosal or any other routes. As used herein the language “pharmaceutically acceptable carrier” includes solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration. Supplementary active compounds can also be incorporated into the compositions. 
     Administration of the vaccine via any method may produce an immune response in an animal or human, it may further produce an antibody response in the animal or human. In a further non-limiting embodiment, the vaccine may produce a protective effect in the animal or human. 
     Generally, the dosage of peptides is in the range of from about 0.01 μg to about 500 mg, from about 0.05 μg to about 200 mg, about 0.075 μg to about 30 mg, about 0.09 μg to about 20 mg, about 0.1 μg to about 10 mg, about 10 μg to about 1 mg, and about 50 μg to about 500 μg. The skilled practitioner can readily determine the dosage and number of dosages needed to produce an effective immune response and/or blocking effect. 
     Compositions Comprising any of SEQ ID NO(s): 1-28 
     A non-limiting composition is provided comprising one or more isolated or synthesized peptides that are 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or more homologous with at least one of the peptides of SEQ ID NO(s): 1-28. A composition is provided comprising one or more isolated or synthesized peptides consisting essentially of or consisting of at least one peptide of SEQ ID NO(s): 1-28. A composition is further provided comprising two, three, four five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, sixteen, seventeen, eighteen, or up to twenty-eight isolated or synthesized peptides of SEQ ID NO(s): 1-28. 
     A composition comprising a mixture of peptides is provided wherein the mixture comprises at least each of the isolated or synthesized peptides of SEQ ID NO(s): 1-9; 1-28; 5, 18, 22, and 23; or 6, 11, 12, 13, 14, 18, 19, 20, 26, 27, and 28. A mixture is provided that is equimolar. A mixture is also provided that is equal by weight. 
     A composition of an aspect of the invention is a therapeutic composition. The therapeutic composition may provide, upon administration to a subject, a therapeutic effect against MERS COV. The therapeutic composition may be administered to any animal susceptible to infection from MERS CoV. The therapeutic composition may be administered to any animal susceptible to MERS CoV, including a human or a camel. 
     Conserved Replikin Peptides Across MERS CoV and Peptide Homologies 
     Identification of conserved Replikin peptides across strains of MERS CoV in different countries has provided for the development of vaccines that may be directed across strains of MERS CoV in different countries. Identification of conserved Replikin peptides in isolates of MERS CoV of any strain may be accomplished in any way known to one of skill in the art now or hereafter. One method is by review of in silico sequences provided at the PubMed website of the National Center for Biotechnology Information. Peptides that share exact identity or 100% homology with earlier identified Replikin peptides may be tracked using computer-searching methods. Peptides that share 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or more homology with an earlier identified Replikin peptide may also be tracked by computer methods. 
     For example, vaccines have now been developed for prevention and treatment of infection from MERS CoV. See, e.g., Examples 1-3 below. The sequences that are used in the vaccine in Example 1 are identically shared among human and camel hosts and conserved from 2012 to 2014. The vaccine in Example 2 likewise comprises sequences shared and conserved. The sequences that are used in the vaccine in Example 3 have now been identified as conserved across countries and strains of MERS CoV. All of these sequences, homologues of these sequences, and proteins, protein fragments, polypeptides, and peptides comprising, consisting essentially of, or consisting of these sequences or their homologues or functional fragments are useful in identifying lethal strains of MERS COV, treating infections from MERS CoV, and developing prophylactic therapies, such as vaccines, against infection from MERS CoV. 
     These proteins, fragment, polypeptides and peptides including any one of SEQ ID NO(s): 1-28 and/or homologues of any one of SEQ ID NO(s): 1-28 and/or functional fragments of SEQ ID NO(s): 1-28 are expected by one of ordinary skill in the art to provide antigenicity, antagonism of replication, and blocking that is comparable to any one of SEQ ID NO(s): 1-28. 
     Homology that is sufficient to produce a useful target for antagonism includes peptides that are 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, or up to 100% homologous with any of SEQ ID NO(s): 1-28. Homology may be determined with peptides wherein gaps exists in the sequence that is being compared to any one of SEQ ID NO(s): 1-28 between amino acids that are identical to those of the peptide chosen from SEQ ID NO(s): 1-28. 
     Sequences that are conserved across strains of MERS CoV are excellent targets for controlling infectivity and lethality. 
     Methods of Designing Vaccines 
     On aspect of the invention also provides methods of designing and making vaccines. For example, the invention provides a method of making a vaccine comprising selecting at least one or more isolated or synthesized Replikin peptides present or identified in an isolate of MERS CoV. Such peptides may include any one or more of SEQ ID NO(s): 1-28 as a component of a vaccine and making said vaccine. The method may comprise selecting from 1 to up to 4 or more isolated or synthesized MERS CoV Replikin peptides as a component of a vaccine. The peptides may be identified in the ORF4b gene area of the virus or may be identified in any area of the genome of MERS CoV. The method may comprise identifying one or more Replikin peptides in an emerging strain of MERS CoV up to about three years before the vaccine is administered, up to about one year before the vaccine is administered, up to about six months before the vaccine is administered, or up to about seven days before the vaccine is made. 
     An emerging strain may be any strain of MERS CoV identified by one of skill in the art as predicted to expand in a population in hosts, or predicted to increase in virulence, morbidity, and/or mortality (lethality) in its hosts. An emerging strain may likewise be a strain of MERS CoV wherein Replikin concentration is observed to be increasing over time. An emerging strain may likewise be a strain of MERS CoV identified within a rising portion of Replikin cycle, following a peak in a Replikin cycle, following a step-wise rise in a Replikin cycle, or identified by a Replikin Count Virus Expansion Index as an emerging strain of virus. See WO 2009/132209, the contents of which are incorporated herein by reference. 
     A method of making a vaccine is also provided comprising: selecting at least one isolated or synthesized protein, protein fragment, polypeptide, or peptide comprising a homologue of a Replikin peptide (including, for example, SEQ ID NO(s): 1-28) as a component of a vaccine; and making said vaccine. An isolated or synthesized protein, protein fragment, polypeptide, or peptide may comprise a peptide that is 30%, 40%, 50%, 60%, 70%, 80%, 90% or 95%, or 100%, homologous with at least one Replikin peptide. At least two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or more homologues of Replikin peptides may be selected. Also, at least two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, or more Replikin peptides may be selected. The isolated or synthesized protein, protein fragment, polypeptide, or peptide may have the same amino acid sequence as at least one protein, protein fragment, polypeptide or peptide identified in an emerging strain of MERS COV up to one, two, or three or more years prior to making said vaccine. The at least one protein, protein fragment, polypeptide or peptide may be identified in an emerging strain of MERS COV one week, one month, two months, three months, four months, five months, or six months prior to making said vaccine. 
     The invention also provides a kit for making a vaccine where the kit includes at least one isolated or synthesized Replikin peptide of MERS CoV (including, for example, at least one peptide of SEQ ID NO(s): 1-28 or homologue of SEQ ID NO(s): 1-28). The kit may also include two, three, four, and up to twenty-eight or more peptides of SEQ ID NO(s): 1-28 or homologues of SEQ ID NO(s): 1-28. 
     Antibodies as Diagnostics and Therapies for Identified Replikin Sequences 
     In another aspect of the invention, isolated Replikin peptides may be used to generate antibodies, antibody fragments, or to generate or identify other binding agents, which may be used, for example, for diagnostic purposes or to provide passive immunity in an individual. See, e.g., U.S. application Ser. No. 11/355,120, filed Feb. 16, 2006 and U.S. application Ser. No. 12/010,027, filed Jan. 18, 2008 (each incorporated herein by reference in their entirety). 
     Various procedures known in the art may be used for the production of antibodies to Replikin sequences or to proteins, protein fragments, polypeptides, or peptides comprising Replikin sequences. Such antibodies include, but are not limited to, polyclonal, monoclonal, chimeric, humanized, single chain, Fab fragments and fragments produced by a Fab expression library. Antibodies that are linked to a cytotoxic agent or signaling moiety may also be generated. Antibodies may also be administered in combination with an antiviral agent. Furthermore, combinations of antibodies to different Replikins may be administered as an antibody cocktail. 
     For the production of antibodies, various host animals may be immunized by injection with a Replikin peptide or a combination of Replikin peptides, including, but not limited to rabbits, mice, rats, and larger mammals. Monoclonal antibodies to Replikins may be prepared using any technique that provides for the production of antibody molecules. These include but are not limited to the hybridoma technique originally described by Kohler and Milstein, (Nature, 1975, 256:495-497), the human B-cell hybridoma technique (Kosbor et al., 1983 , Immunology Today,  4:72), and the EBV hybridoma technique (Cole et al.,  Monoclonal Antibodies and Cancer Therapy , Alan R. Liss, Inc., pp. 77-96). In addition, techniques developed for the production of chimeric antibodies (Morrison et al., 1984 , Proc. Nat. Acad. Sci USA,  81:6851-6855) or other techniques may be used. Alternatively, techniques described for the production of single chain antibodies (U.S. Pat. No. 4,946,778) can be adapted to produce Replikin-specific single chain antibodies. Antibody fragments that contain binding sites for a Replikin may be generated by known techniques. For example, such fragments include but are not limited to F(ab′)2 fragments which can be produced by pepsin digestion of the antibody molecules and or fragments that can be generated by reducing the disulfide bridges of F(ab′)2 fragments. Alternatively, Fab expression libraries can be generated (Huse et al., 1989, Science, 246:1275-1281) to allow rapid and easy identification of monoclonal Fab fragments with the desired specificity. 
     Binding agents are provided including an antibody, antibody fragment, or binding agent that binds to at least a portion of an amino acid sequence of at least one protein, protein fragment, polypeptide, or peptide comprising at least one peptide A, where peptide A is at least 30%, 40%, 50%, 60%, 70%, 80%, 90% or 95%, or 100%, homologous with at least one Replikin peptide of MERS CoV, which may include, for example, at least one Replikin peptide of SEQ ID NO(s): 1-28. The amino acid sequence of a protein fragment, polypeptide, or peptide may partially match the amino acid sequence of an expressed whole protein where at least one, five, ten, twenty, thirty, forty, fifty, one hundred, two hundred, three hundred, four hundred, five hundred or more amino acid residues of the amino acid sequence of the expressed whole protein are not present in the protein fragment, polypeptide, or peptide. The amino acid sequence of the protein fragment, polypeptide, or peptide may also partially match the amino acid sequence of an expressed whole protein where at least one, ten, twenty, thirty, forty, fifty, sixty, seventy, eighty, ninety, one hundred, one hundred fifty, two hundred, two hundred fifty, three hundred, three hundred fifty, four hundred, four hundred fifty, five hundred, five hundred fifty or more amino acid residues of the amino acid sequence of at least one terminus of the expressed whole protein are not present at least one terminus of said protein fragment, polypeptide, or peptide. 
     Binding agents are also provided including an antibody, antibody fragment, or binding agent that binds to at least a portion of an amino acid sequence that is 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or more homologous with at least one Replikin peptide of MERS CoV. In a non-limiting embodiment, the length of peptide A may be no more than one, five, ten, twenty, thirty, forty, or fifty amino acid residues longer than the identified Replikin sequence with which it is homologous. Binding agents are also provided that bind to at least a portion of an amino acid sequence of at least one of SEQ ID NO(s): 1-28. 
     Binding agents may specifically or preferentially bind to the target protein, protein fragment, polypeptide, or peptide. Binding agents may specifically or preferentially bind to a homologue of at least one of SEQ ID NO(s): 1-28. Binding agents may likewise specifically or preferentially bind to a peptide consisting of any one of SEQ ID NO(s): 1-28. Binding agents may also specifically or preferentially bind to a portion of a peptide consisting of any one of SEQ ID NO(s): 1-28 including a single amino acid within a homologue of SEQ ID NO(s): 1-28, two amino acids, three amino acids, four amino acids, five amino acids, or any number of amino acids spread within or outside a homologue. 
     Nucleic Acids and Compositions of Nucleic Acids 
     An isolated or synthesized nucleic acid sequence is also provided that encodes a protein, protein fragment, polypeptide, or peptide comprising at least one peptide A, where peptide A is at least 30%, 40%, 50%, 60%, 70%, 80%, 90% or 95%, or 100%, homologous with at least one Replikin peptide of MERS CoV. The at least one Replikin peptide may be any peptide of SEQ ID NO(s): 1-28. A nucleic acid sequence may also encode a protein, a protein fragment, a polypeptide, or a peptide where the amino acid sequence of the protein, protein fragment, polypeptide, or peptide partially matches the amino acid sequence of an expressed whole protein and at least one, two, three, four, five, ten, twenty, thirty, forty, fifty, one hundred, two hundred, three hundred, four hundred, five hundred or more amino acid residues of the amino acid sequence of the expressed whole protein are not present in the protein fragment, polypeptide, or peptide. Further, the amino acid sequence of the protein, protein fragment, polypeptide, or peptide may partially match the amino acid sequence of an expressed whole protein where at least one, two, three, four, five, ten, twenty, thirty, forty, fifty, sixty, seventy, eighty, ninety, one hundred, one hundred fifty, two hundred, two hundred fifty, three hundred, three hundred fifty, four hundred, four hundred fifty, five hundred, five hundred fifty or more amino acid residues of the amino acid sequence of at least one terminus of the expressed whole protein may not be present at least one terminus of the protein, protein fragment, polypeptide, or peptide 
     An isolated or synthesized nucleic acid sequence may also encode a peptide consisting of 7 to about 50 amino acid residues comprising at least one Replikin peptide, which may be one of the peptide sequences of SEQ ID NO(s): 1-28. It may also encode a peptide that is at least 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or more homologous with at least one of the peptide sequences of SEQ ID NO(s): 1-28. It may also encode a peptide consisting essentially of or consisting of at least one of the peptide sequences of SEQ ID NO(s): 1-28. 
     One aspect of the invention further provides an immunogenic composition that comprises an isolated or synthesized nucleic acid provided above. One aspect of the invention further provides a vaccine against MERS CoV comprising an isolated or synthesized nucleic acid provided above. 
     Anti-Sense Nucleic Acids and siRNA 
     One aspect of the invention further provides a nucleic acid sequence that is antisense to a nucleic acid that encodes for any Replikin peptide present in or identified in an MERS CoV isolate. This may include one of SEQ ID NO(s): 1-28 or a small interfering nucleic acid sequence that interferes with a nucleic acid sequence that is 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or more homologous with a nucleic acid that encodes any Replikin peptide of MERS CoV including, for example, any one of SEQ ID NO(s): 1-28 or is 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or more homologous with a nucleic acid that is antisense to a nucleic acid that encodes for any one of SEQ ID NO(s): 1-28. 
     The nucleotide sequence of the invention may be used in hybridization assays of biopsied tissue or blood, e.g., Southern or Northern analysis, including in situ hybridization assays, to diagnose the presence of a particular MERS CoV strain in a tissue sample or an environmental sample, for example. The present invention also provides kits containing antibodies specific for particular Replikins that are present in a particular isolate of MERS CoV, or containing nucleic acid molecules (sense or antisense) that hybridize specifically to a particular Replikin sequence, and optionally, various buffers and/or reagents needed for diagnosis. 
     Also within the scope of an aspect of the invention are oligoribonucleotide sequences that include antisense RNA and DNA molecules and ribozymes that function to inhibit the translation of Replikin-containing mRNA. Both antisense RNA and DNA molecules and ribozymes may be prepared by any method known in the art. The antisense molecules can be incorporated into a wide variety of vectors for delivery to a subject. The skilled practitioner can readily determine the best route of delivery, although generally intravenous or intramuscular delivery is routine. The dosage amount is also readily ascertainable. 
     An aspect of the invention further provides antisense nucleic acid molecules that are complementary to a nucleic acid of the invention, wherein the antisense nucleic acid molecule is complementary to a nucleotide sequence encoding a peptide of the invention. In particular the nucleic acid sequence may be anti-sense to a nucleic acid sequence that has been demonstrated to be conserved over a period of six months to one or more years and/or which are present in a strain of MERS CoV shown to have an increase in concentration of Replikin sequences relative to Replikin concentration in other MERS CoV strains. 
     An aspect of the invention also provides compositions comprising RNAi-inducing entities used to inhibit or reduce MERS CoV infection or replication including small interfering RNA, which is a class of about 10 to about 50 and often about 20 to about 25 nucleotide-long double-stranded RNA molecules. siRNA is involved in the RNA interference pathway, where it interferes with the expression of a specific gene such as the ORF4b gene area of MERS CoV. siRNAs also act in RNAi-related pathways, e.g., as an antiviral mechanism. 
     An effective amount of an RNAi-inducing entity is delivered to a cell or organism prior to, simultaneously with, or after exposure to MERS CoV. A dosage may be sufficient to reduce or delay one or more symptoms of MERS CoV infection. Compositions of the invention may comprise a single siRNA species targeted to a target transcript or may comprise a plurality of different siRNA species targeting one or more target transcripts. 
     One aspect of the invention provides a small interfering nucleic acid sequence that is about 10 to about 50 nucleic acids in length and is 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or more homologous with a nucleic acid that encodes for any portion of an MERS CoV Replikin peptide including, for example, any portion of SEQ ID NO(s): 1-28 or is 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95% or more homologous with a nucleic acid that is antisense to a nucleic acid that encodes for any portion of a Replikin peptide, including, for example, a portion of one of SEQ ID NO(s): 1-28. In a further non-limiting embodiment, the nucleic acid sequence is about 15 to about 30 nucleic acids. In a further non-limiting embodiment, the nucleic acid sequence is about 20 to about 25 nucleic acids. In a further non-limiting embodiment, the nucleic acid sequence is about 21 nucleic acids. 
     Advance Replikin-Based Information on Pathogenic Outbreaks Provides for Rapid Production of Vaccines 
     Advance information concerning Replikin peptides in expanding strains of pathogen allows for the rapid production of specific effective synthetic vaccines using one, or a combination, of Replikin peptides. Such synthetic vaccines have been demonstrated in rabbits, chickens, and shrimp. See, e.g., Examples 1-3 herein, Examples 6 and 7 of U.S. application Ser. No. 11/355,120, filed Feb. 16, 2006, and Example 2 of U.S. application Ser. No. 12/108,458, filed Apr. 23, 2008. For example, a mixture of Replikin peptides administered orally to shrimp provided up to a 91% protective effect for shrimp challenged with taura syndrome virus. Taura syndrome virus is an often-lethal rapidly replicating pathogen that has a significant negative impact on the shrimp industry. 
     Synthetic Replikin vaccines have also been demonstrated in the H5N1 strain of influenza virus in chickens. For example, in a test of chickens administered a mixture of twelve H5N1 Replikin peptides from the hemagglutinin and pB1 gene areas intranasally, intraocularly, and by spray inhalation and challenged with low pathogenic H5N1 influenza isolated from a black duck in the state of North Carolina in the United States, a protective effect was observed at both the entry site of influenza (diminished antibody production in the serum was observed as compared to a control) and at excretion sites of influenza (influenza virus was not observed excreted in feces or saliva from treated chickens as compared to a control). See Example 1 of U.S. application Ser. No. 12/581,112, filed Oct. 16, 2009 (incorporated herein by reference). 
     Administration of Replikin peptides in both shrimp and chickens appears to have provided a notable measure of mucosal immunity. For example, in Example 2 of U.S. application Ser. No. 12/108,458, a mixture of Replikin peptides was administered by mouth to shrimp later challenged with taura syndrome virus. The 91% protective effect of the vaccine is expected to have been a result, at least in part, of a mucosal immune-like response in the gut of the shrimp. 
     Likewise, in chickens, the administration of a mixture of Replikin peptides provided a protective effect against entry of the H5N1 virus. For example, as may be seen in Example 1 of U.S. application Ser. No. 12/581,112, filed Oct. 16, 2009 (incorporated herein by reference), three of six vaccinated chickens, when inoculated with H5N1 virus, produced no measurable amount of antibodies against H5N1 in their serum. Instead, the virus was apparently blocked by mucosal immunity from even entering the chickens&#39; system. Some virus apparently entered the system of the chickens but was then blocked intracellularly. While the applicants do not wish to be bound by theory, the virus may have been blocked in its intracellular transport to the RNA or in synthesis of virus on RNA or in transport from the RNA to excretion. Wherever the block occurs, the fact is that the examination of the excreta of the chicken showed complete absence of virus. For those three chickens in which a serum immune response was measured (that is, virus did enter their system), the vaccine additionally provided a protective effect against replication of the virus in the chickens&#39; system (no virus was excreted in the feces or saliva of the chickens). As such, mucosal immunity, in addition to other immunities, is an important aspect of the immunity imparted by Replikin-based vaccines. 
     Example 1 
     Synthetic Replikin Vaccine Against MERS CoV Using Selected Peptides Identically-Shared Among Camels and Humans 
     A synthetic Replikin vaccine containing an approximately equal-parts-by-weight mixture of nine MERS CoV Replikin peptides was designed for use against relatively-lethal isolates of MERS CoV. The sequences were found to be identical, shared Replikin structures in MERS CoV in dromedary camels ( Camelus dromedaries ) and humans. The vaccine was designed as a best-fit synthetic vaccine against MERS CoV for identified isolates from 2012 to 2014 shared among these two host species. The vaccine makes possible immediate MERS-CoV Replikins Synthetic Vaccine™ tests in camels and rapid interruption of the spread of the MERS-CoV coronavirus, more lethal to humans than SARS 2003. 
     The vaccine was engineered to inhibit the lethality of relatively-lethal strains of MERS CoV and to attack the camel reservoir of the virus as well as protect humans. 
     The vaccine comprises a mixture of the following nine Replikin peptides in sterile water: 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 1) 
               
            
           
           
               
               
               
            
               
                   
                 (1) 
                 KQKAPKH 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 2) 
               
            
           
           
               
               
               
            
               
                   
                 (2) 
                 KSAGHPFNK 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 3) 
               
            
           
           
               
               
               
            
               
                   
                 (3) 
                 KRSHSPTKK 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 4) 
               
            
           
           
               
               
               
            
               
                   
                 (4) 
                 HSPTKKLRYVK 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 5) 
               
            
           
           
               
               
               
            
               
                   
                 (5) 
                 KARKRSHSPTK 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 6) 
               
            
           
           
               
               
               
            
               
                   
                 (6) 
                 KHVEVFTDGK 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 7) 
               
            
           
           
               
               
               
            
               
                   
                 (7) 
                 HKWKMVVCDK 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 8) 
               
            
           
           
               
               
               
            
               
                   
                 (8) 
                 KKHVEVFTDGK 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 9) 
               
            
           
           
               
               
               
            
               
                   
                 (9) 
                 KDAAAAKNKMRHK 
               
            
           
         
       
     
     The vaccine is administered to a camel and an immune response is detected. In camels where some antibody is detected prior to administration of the vaccine, administration of the vaccine produces an increase in antibody response and an increase in immune protection. Challenge of the camel with MERS-CoV isolates results in a protective effect against the challenge including reduction in entrance of virus into camel at inoculation, reduction in replication of virus in camel system, and blocking of excretion of virus from camel in body fluids. The camel is protected from infection. The vaccine is likewise tested in humans, providing an immune response, a blocking effect, and a protective effect. 
     The vaccine may be administered to an animal or human susceptible, exposed to, or suffering from infection of MERS CoV. The blocking mechanism of the vaccine provides a therapeutic and prophylactic effect. The immune response generated by the vaccine provides a prophylactic effect. Because each of the sequences comprised in the vaccine is related to rapid replication and lethality of the virus and provides an immune and a blocking response upon administration to a subject at sufficient volume and concentration, each sequence may be used as an individual active component in a vaccine against MERS-CoV; a mixture of peptides is not necessary to provide an effective vaccine. 
     Example 2 
     Synthetic Replikin Vaccine Against MERS CoV Using Further Selected Peptides Shared Among Camels and Humans 
     A synthetic Replikin vaccine containing an approximately equal-parts-by-weight mixture of twenty-eight MERS CoV Replikin peptides was designed for use against relatively lethal isolates of MERS CoV. The sequences were found to be shared Replikin structures in MERS CoV in dromedary camels ( Camelus dromedaries ) and humans. The vaccine was designed as a synthetic vaccine against MERS CoV for identified isolates from 2012 to 2014 shared among these two host species. The vaccine makes possible immediate MERS-CoV Replikins Synthetic Vaccine™ tests in camels and rapid interruption of the spread of the MERS-CoV coronavirus, more lethal to humans than SARS 2003. 
     The vaccine was engineered to inhibit the lethality of relatively-lethal strains of MERS CoV. 
     The vaccine comprises a mixture of the following twenty-eight Replikin peptides in sterile water: 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 1) 
               
               
                   
                 KQKAPKH 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 2) 
               
               
                   
                 KSAGHPFNK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 3) 
               
               
                   
                 KRSHSPTKK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 4) 
               
               
                   
                 HSPTKKLRYVK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 5) 
               
               
                   
                 KARKRSHSPTK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 6) 
               
               
                   
                 KHVEVFTDGK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 7) 
               
               
                   
                 HKWKMVVCDK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 8) 
               
               
                   
                 KKHVEVFTDGK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 9) 
               
               
                   
                 KDAAAAKNKMRHK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 10) 
               
               
                   
                 KSVVRHLGVTK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 11) 
               
               
                   
                 KFYQHVINGCK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 12) 
               
               
                   
                 KQVHQVQLTDK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 13) 
               
               
                   
                 KGDSCSSNCKH 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 14) 
               
               
                   
                 HARLKGGLILK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 15) 
               
               
                   
                 KAMLLKKEPLLYVPIRLAGH 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 16) 
               
               
                   
                 KHLVPLMHK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 17) 
               
               
                   
                 KYYAFLNKH 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 18) 
               
               
                   
                 KAAVHKWK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 19) 
               
               
                   
                 KLNPSEDFIKH 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 20) 
               
               
                   
                 KFCDHMVK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 21) 
               
               
                   
                 KPGHAMPSLFK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 22) 
               
               
                   
                 KNKMRHK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 23) 
               
               
                   
                 KRSHSPTK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 24) 
               
               
                   
                 KDAAAAKNKMRH 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 25) 
               
               
                   
                 KMRHKRTSTK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 26) 
               
               
                   
                 HVERKDVPYPK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 27) 
               
               
                   
                 KGMQLLHTK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 28) 
               
               
                   
                 KEGSSVTLKH. 
               
            
           
         
       
     
     The vaccine is administered to a camel and an immune response is detected. Challenge of the camel with MERS-CoV isolates results in a protective effect against the challenge including reduction in entrance of virus into camel at inoculation, reduction in replication of virus in camel system, and blocking of excretion of virus from camel in body fluids. The camel is protected from infection. The vaccine is likewise tested in humans, providing an immune response, a blocking effect, and a protective effect. 
     The vaccine may be administered to an animal or human susceptible, exposed to, or suffering from infection of MERS CoV. The blocking mechanism of the vaccine provides a therapeutic and prophylactic effect. The immune response generated by the vaccine provides a prophylactic effect. Because each of the sequences comprised in the vaccine is related to rapid replication and lethality of the virus and provides an immune and a blocking response upon administration to a subject at sufficient volume and concentration, each sequences may be used as an individual active component in a vaccine against MERS-CoV; a mixture of peptides is not necessary to provide an effective vaccine. 
     Example 3 
     Synthetic Replikin Vaccine Against MERS CoV 
     A synthetic Replikin vaccine containing an approximately equal-parts-by-weight mixture of four MERS CoV Replikin peptides was designed for use against relatively lethal isolates of MERS CoV. The vaccine was engineered from sequences confirmed to be conserved across regions (countries) and across time. Conservation was particularly noted in the key amino acid residues of the Replikin sequence, namely, lysine and histidine amino acid residues. The vaccine was engineered to inhibit the lethality of relatively lethal strains of MERS CoV. 
     The vaccine comprises a mixture of the following four Replikin peptides in sterile water: 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 22) 
               
            
           
           
               
               
               
            
               
                   
                 (1) 
                 KNKMRHK; 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 18) 
               
            
           
           
               
               
               
            
               
                   
                 (2) 
                 KAAVHKWK; 
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 23) 
               
            
           
           
               
               
               
            
               
                   
                 (3) 
                 KRSHSPTK; 
               
               
                   
                 and 
                   
               
               
                   
                   
               
            
           
           
               
               
            
               
                   
                 (SEQ ID NO: 5) 
               
            
           
           
               
               
               
            
               
                   
                 (4) 
                 KARKRSHSPTK. 
               
            
           
         
       
     
     The vaccine may be administered to an animal or human susceptible, exposed to, or suffering from infection of MERS CoV. The blocking mechanism of the vaccine provides a therapeutic and prophylactic effect. The immune response generated by the vaccine provides a prophylactic effect. 
     Example 4 
     Analysis of Accession No. AHX71944 for Replikin Sequences Conserved in Camel Host and Shared with Isolates in Human Hosts 
       
     
       
         
           
               
               
            
               
                 PubMed Code: 
                   
               
               
                 AHX71944 
               
               
                 Description: 
               
               
                 Isolation and Characterization of MERS Coronavirus from a Dromedary Camel, Qatar, 2014 
               
               
                 Direct Submission 
               
               
                 Isolated: 
               
               
                 2014 in Qatar 
               
               
                 Source: 
               
               
                 Middle East respiratory syndrome coronavirus (MERS-CoV) 
               
               
                 Strain: 
               
               
                 MERS-CoV 
               
               
                 (SEQ ID NO: 29) 
                   
               
               
                 m 1  s 2  f 3  v 4  a 5  g 6  v 7  t 8  a 9  q 10  g 11  a 12  r 13  g 14  t 15  y 16  r 17  a 18  a 19  l 20  n 21  s 22  e 23  k 24  h 25  q 26  d 27  h 28   
                   
               
               
                 v 29  s 30  l 31  t 32  v 33  p 34  l 35  e 36  g 37  s 38  g 39  n 40  l 41  v 42  e 43  k 44  l 45  s 46  p 47  w 48  f 49  m 50  d 51  g 52  e 53  n 54   
               
               
                 a 55  y 56  e 57  v 58  v 59  k 60  a 61  m 62  l 63  l 64  k 65  k 66  e 67  p 68  k 69  l 70  y 71  v 72  p 73  i 74  r 75  k 76  a 77  g 78  h 79  t 80   
               
               
                 r 81  h 82  k 83  p 84  g 85  p 86  r 87  v 88  y 89  k 90  v 91  e 92  r 93  l 94  i 95  a 96  e 97  e 98  n 99  p 100  f 101  m 102  e 103  n 104  q 105   
               
               
                   106  a 107  y 108  s 109  s 110  s 111  a 112  n 113  g 114  s 115  l 116  v 117  g 118  t 119  t 120  l 121  q 122  g 123  k 124  p 125  i 126  g 127   
               
               
                 m 128  f 129  f 130  p 131  y 132  d 133  i 134  e 135  l 136  e 137  t 138  g 139  k 140  q 141  n 142  i 143  l 144  l 145  r 146  k 147  y 148  g 149   
               
               
                 r 150  g 151  g 152  y 153  h 154  y 155  t 156  p 157  f 158  h 159  y 160  e 161  r 162  d 163  n 164  t 165  s 166  e 167  p 168  e 169  w 170  m 171   
               
               
                 d 172  d 173  f 174  e 175  a 176  d 177  p 178  k 179  g 180  k 181  y 182  a 183  q 184  n 185  l 186  l 187  k 188  k 189  l 190  i 191  g 192  g 193   
               
               
                 d 194  e 195  t 196  p 197  e 198  d 199  q 200  y 201  m 202  c 203  g 204  v 205  d 206  g 207  k 208  p 209  i 210  s 211  a 212  y 213  a 214  f 215   
               
               
                 l 216  m 217  a 218  k 219  d 220  g 221  i 222  t 223  k 224  l 225  a 226  d 227  v 228  e 229  a 230  d 231  v 232  a 233  a 234  r 235  a 236  d 237   
               
               
                 d 238  e 239  g 240  f 241  i 242  t 243  l 244  k 245  n 246  n 247  l 248  y 249  r 250  l 251  v 252  w 253  h 254  v 255  e 256  r 257  k 258  d 259   
               
               
                 v 260  p 261  y 262  p 263  k 264  q 265  s 266  i 267  f 268  t 269  i 270  n 271  s 272  v 273  v 274  q 275  k 276  d 277  g 278  v 279  e 280  n 281   
               
               
                 t 282  p 283  p 284  h 285  y 286  f 287  t 288  l 289  g 290  e 291  k 292  i 293  l 294  t 295  l 296  t 297  p 298  r 299  n 300  k 301  w 302  s 303   
               
               
                 g 304  v 305  s 306  d 307  l 308  s 309  l 310  k 311  q 312  k 313  l 314  l 315  y 316  t 317  f 318  y 319  g 320  k 321  e 322  s 323  l 324  e 325   
               
               
                 n 326  p 327  t 328  y 329  i 330  y 331  h 332  s 333  a 334  f 335  i 336  e 337  e 338  g 339  s 340  e 341  g 342  n 343  d 344  s 345  w 346  l 347   
               
               
                 t 348  g 349  n 350  a 351  i 352  q 353  g 354  f 355  a 356  e 357  g 358  e 359  g 360  a 361  s 362  y 363  t 364  a 365  n 366  d 367  v 368  e 369   
               
               
                 e 370  q 371  s 372  s 373  g 374  m 375  i 376  k 377  p 378  n 379  a 380  l 381  l 382  e 383  a 384  t 385  e 386  p 387  f 388  a 389  k 390  g 391   
               
               
                 d 392  s 393  e 394  s 395  s 396  n 397  e 398  k 399  h 400  s 401  v 402  a 403  q 404  l 405  v 406  s 407  y 408  l 409  s 410  e 411  r 412  e 413   
               
               
                 n 414  v 415  i 416  a 417  d 418  s 419  k 420  s 421  f 422  t 423  l 424  i 425  f 426  g 427  g 428  v 429  a 430  y 431  a 432  y 433  f 434  g 435   
               
               
                 e 436  e 437  e 438  g 439  t 440  m 441  y 442  f 443  e 444  p 445  r 446  a 447  k 448  s 449  e 450  v 451  s 452  r 453  i 454  g 455  d 456  s 457   
               
               
                 i 458  f 459  t 460  g 461  e 462  t 463  g 464  s 465  w 466  p 467  k 468  v 469  t 470  q 471  i 472  a 473  n 474  m 475  f 476  l 477  e 478  q 479   
               
               
                 t 480  q 481  h 482  s 483  l 484  n 485  f 486  v 487  g 488  e 489  f 490  v 491  v 492  n 493  d 494  v 495  v 496  l 497  a 498  i 499  l 500  a 501   
               
               
                 t 502  t 503  t 504  t 505  n 506  v 507  d 508  k 509  i 510  r 511  q 512  l 513  l 514  k 515  g 516  v 517  t 518  l 519  d 520  k 521  l 522  r 523   
               
               
                 d 524  y 525  l 526  a 527  d 528  y 529  d 530  v 531  a 532  v 533  t 534  a 535  g 536  p 537  f 538  m 539  d 540  n 541  a 542  i 543  n 544  v 545   
               
               
                 g 546  g 547  t 548  g 549  l 550  q 551  y 552  a 553  a 554  i 555  t 556  a 557  p 558  y 559  e 560  v 561  l 562  t 563  g 564  l 565  g 566  e 567   
               
               
                 s 568  f 569  k 570  k 571  v 572  a 573  t 574  i 575  p 576  y 577  k 578  v 579  e 580  n 581  s 582  v 583  k 584  d 585  t 586  l 587  t 588  y 589   
               
               
                 y 590  a 591  h 592  s 593  v 594  l 595  y 596  r 597  v 598  f 599  p 600  y 601  d 602  m 603  d 604  s 605  g 606  v 607  s 608  s 609  f 610  s 611   
               
               
                 e 612  l 613  l 614  f 615  d 616  e 617  v 618  d 619  l 620  s 621  v 622  a 623  s 624  t 625  y 626  f 627  l 628  v 629  r 630  l 631  l 632  q 633   
               
               
                 d 634  k 635  t 636  g 637  d 638  f 639  m 640  s 641  t 642  i 643  i 644  t 645  s 646  e 647  q 648  t 649  a 650  v 651  s 652  k 653  l 654  l 655   
               
               
                 d 656  t 657  e 658  f 659  e 660  a 661  t 662  e 663  a 664  t 665  f 666  n 667  f 668  l 669  l 670  d 671  l 672  a 673  g 674  l 675  f 676  r 677   
               
               
                 i 678  f 679  l 680  r 681  n 682  a 683  y 684  v 685  y 686  t 687  s 688  q 689  g 690  f 691  v 692  v 693  v 694  n 695  g 696  k 697  v 698  s 699   
               
               
                 t 700  l 701  v 702  k 703  q 704  v 705  l 706  d 707  l 708  l 709  n 710  k 711  g 712  m 713  q 714  l 715  l 716  h 717  t 718  k 719  e 720  s 721   
               
               
                 w 722  a 723  g 724  s 725  n 726  i 727  s 728  a 729  e 730  i 731  y 732  s 733  g 734  r 735  e 736  s 737  l 738  i 739  f 740  p 741  s 742  g 743   
               
               
                 t 744  y 745  y 746  c 747  v 748  t 749  t 750  k 751  a 752  k 753  s 754  v 755  q 756  q 757  d 758  l 759  d 760  v 761  i 762  l 763  p 764  g 765   
               
               
                 e 766  f 767  s 768  k 769  k 770  q 771  l 772  g 773  l 774  l 775  q 776  p 777  t 778  d 779  n 780  s 781  t 782  t 783  v 784  s 785  v 786  t 787   
               
               
                 v 788  s 789  s 790  n 791  m 792  v 793  e 794  t 795  v 796  v 797  g 798  q 799  l 800  e 801  q 802  t 803  n 804  m 805  h 806  s 807  p 808  d 809   
               
               
                 e 810  i 811  v 812  g 813  d 814  y 815  v 816  i 817  i 818  s 819  e 820  k 821  l 822  f 823  v 824  r 825  s 826  k 827  e 828  e 829  d 830  g 831   
               
               
                 f 832  a 833  f 834  y 835  p 836  a 837  e 838  t 839  n 840  g 841  h 842  a 843  v 844  p 845  t 846  l 847  f 848  r 849  l 850  k 851  g 852  g 853   
               
               
                 a 854  p 855  v 856  k 857  k 858  v 859  a 860  f 861  g 862  g 863  d 864  q 865  v 866  h 867  e 868  v 869  a 870  a 871  v 872  r 873  s 874  v 875   
               
               
                 t 876  v 877  e 878  y 879  n 880  i 881  h 882  a 883  v 884  l 885  d 886  t 887  l 888  l 889  a 890  s 891  s 892  s 893  l 894  r 895  t 896  f 897   
               
               
                 v 898  v 899  d 900  k 901  s 902  l 903  s 904  i 905  e 906  e 907  f 908  a 909  d 910  v 911  v 912  k 913  e 914  q 915  v 916  s 917  d 918  l 919   
               
               
                 l 920  v 921  k 922  l 923  l 924  r 925  g 926  m 927  p 928  i 929  p 930  d 931  f 932  d 933  l 934  d 935  d 936  f 937  i 938  d 939  a 940  p 941   
               
               
                 e 942  y 943  e 944  f 945  p 946  a 947  e 948  g 949  d 950  a 951  s 952  w 953  s 954  s 955  t 956  m 957  i 958  f 959  s 960  l 961  h 962  p 963   
               
               
                 e 964  e 965  e 966  d 967  e 968  e 969  e 970  s 971  e 972  v 973  e 974  a 975  s 976  d 977  l 978  e 979  e 980  g 981  e 982  s 983  e 984  c 985   
               
               
                 i 986  s 987  e 988  t 989  s 990  t 991  e 992  q 993  v 994  d 995  v 996  s 997  h 998  e 999  i 1000  s 1001  d 1002  d 1003  e 1004  w 1005  a 1006   
               
               
                 a 1007  a 1008  v 1009  d 1010  e 1011  a 1012  f 1013  p 1014  l 1015  d 1016  e 1017  a 1018  e 1019  d 1020  e 1021  t 1022  e 1023  s 1024  e 1025   
               
               
                 q 1026  e 1027  e 1028  a 1029  q 1030  p 1031  e 1032  e 1033  e 1034  p 1035  e 1036  e 1037  d 1038  i 1039  a 1040  q 1041  e 1042  e 1043  i 1044   
               
               
                 a 1045  d 1046  t 1047  l 1048  q 1049  e 1050  t 1051  p 1052  e 1053  e 1054  5 1055  d 1056  t 1057  e 1058  e 1059  v 1060  p 1061  p 1062  q 1063   
               
               
                 e 1064  e 1065  k 1066  l 1067  p 1068  s 1069  e 1070  p 1071  q 1072  t 1073  i 1074  q 1075  p 1076  e 1077  e 1078  k 1079  e 1080  e 1081  a 1082   
               
               
                 p 1083  e 1084  y 1085  e 1086  a 1087  d 1088  t 1089  e 1090  q 1091  t 1092  q 1093  s 1094  e 1095  t 1096  e 1097  k 1098  p 1099  k 1100  r 1101   
               
               
                 l 1102  r 1103  k 1104  k 1105  r 1106  n 1107  e 1108  d 1109  p 1110  l 1111  s 1112  n 1113  f 1114  e 1115  h 1116  k 1117  v 1118  i 1119  t 1120   
               
               
                 e 1121  c 1122  v 1123  t 1124  i 1125  v 1126  l 1127  g 1128  d 1129  a 1130  i 1131  q 1121  v 1133  a 1134  k 1135  c 1136  y 1137  g 1138  e 1139   
               
               
                 s 1140  v 1141  l 1142  v 1143  n 1144  a 1145  a 1146  n 1147  t 1148  h l149  l 1150  k 1151  h 1152  g 1153  g 1154  g 1155  i 1156  a 1157  g 1158   
               
               
                 a 1159  i 1160  n 1161  a 1162  a 1163  s 1164  k 1165  g 1166  a 1167  v 1168  q 1169  k 1170  e 1171  s 1172  d 1173  e 1174  y 1175  i 1176  l 1177   
               
               
                 a 1178  k 1179  g 1180  p 1181  l 1182  q ll83  v 1184  g 1185  d 1186  s 1187  v 1188  l 1189  l 1190  q 1191  g 1192  h 1193  s 1194  l 1195  a 1196   
               
               
                 k 1197  n 1198  i 1199  l 1200  h 1201  v 1202  v 1203  g 1204  p 1205  d 1206  a 1207  r 1208  a 1209  k 1210  q 1211  d 1212  v 1213  s 1214  l 1215   
               
               
                 l 1216  s 1217  k 1218  c 1219  y 1220  k 1221  a 1222  m 1223  n 1224  a 1225  y 1226  p 1227  l 1228  v 1229  v 1230  t 1231  p 1232  p 1233  v 1234   
               
               
                 s 1235  a 1236  g 1237  i 1238  f 1239  g 1240  v 1241  k 1242  p 1243  g 1244  v 1245  s 1246  f 1247  d 1248  y 1249  l 1250  i 1251  r 1252  e 1253   
               
               
                 a 1254  k 1255  t 1256  r 1257  v 1258  l 1259  v 1260  v 1261  v 1262  n 1263  s 1264  q 1265  d 1266  v 1267  y 1268  k 1269  s 1270  l 1271  l 1272   
               
               
                 i 1273  v 1274  d 1275  i 1276  p 1277  q 1278  s 1279  l 1280  t 1281  f 1282  s 1283  y 1284  d 1285  g 1286  l 1287  r 1288  g 1289  a 1290  i 1291   
               
               
                 r 1292  k 1293  a 1294  k 1295  d 1296  y 1297  g 1298  f 1299  t 1300  v 1301  f 1302  v 1303  c 1304  t 1305  d 1306  n 1307  s 1308  a 1309  n 1310   
               
               
                 t 1311  k 1312  v 1313  l 1314  r 1315  n 1316  k 1317  g 1318  v 1319  d 1320  y 1321  t 1322  k 1323  k 1324  f 1325  l 1326  t 1327  v 1328  d 1329   
               
               
                 g 1330  v 1331  q 1332  y 1333  y 1334  c 1335  y 1336  t 1337  s 1338  k 1339  d 1340  t 1341  l 1342  d 1343  d 1344  i 1345  l 1346  q 1347  q 1348   
               
               
                 a 1349  n 1350  k 1351  s 1352  v 1353  g 1354  i 1355  i 1356  s 1357  m 1358  p 1359  l 1360  g 1361  y 1362  v 1363  s 1364  h 1365  g 1366  l 1367   
               
               
                 f 1368  l 1369  i 1370  q 1371  a 1372  g 1373  s 1374  v 1375  v 1376  r 1377  r 1378  v 1379  n 1380  v 1381  p 1382  y 1383  v 1384  c 1385  l 1386   
               
               
                 l 1387  a 1388  n 1389  k 1390  e 1391  q 1392  e 1393  a 1394  i 1395  l 1396  m 1397  s 1398  e 1399  d 1400  v 1401  k 1402  l 1403  n 1404  p 1405   
               
               
                 s 1406  e 1407  d 1408  f 1409  i 1410  k 1411  h 1412  v 1413  r 1414  t 1415  n 1416  g 1417  g 1418  y 1419  n 1420  s 1421  w 1422  h 1423  l 1424   
               
               
                 v 1425  e 1426  g 1427  e 1428  l 1429  l 1430  v 1431  q 1432  d 1433  l 1434  r 1435  l 1436  n 1437  k 1438  l 1439  l 1440  h 1441  w 1442  s 1443   
               
               
                 d 1444  q 1445  t 1446  i 1447  c 1448  y 1449  k 1450  d 1451  s 1452  v 1453  f 1454  y 1455  v 1456  v 1457  k 1458  n 1459  s 1460  t 1461  a 1462   
               
               
                 f 1463  p 1464  f 1465  e 1466  t 1467  l 1468  s 1469  a 1470  c 1471  r 1472  a 1473  y 1474  l 1475  d 1476  s 1477  r 1478  t 1479  t 1480  q 1481   
               
               
                 q 1482  l 1483  t 1484  i 1485  e 1486  v 1487  l 1488  v 1489  t 1490  v 1491  d 1492  g 1493  v 1494  n 1495  f 1496  r 1497  t 1498  v 1499  v 1500   
               
               
                 l 1501  n 1502  n 1503  k 1504  n 1505  t 1506  y 1507  r 1508  s 1509  q 1510  l 1511  g 1512  c 1513  v 1514  f 1515  f 1516  n 1517  g 1518  a 1519   
               
               
                 d 1520  i 1521  s 1522  d 1523  t 1524  i 1525  p 1526  d 1527  e 1528  k 1529  q 1530  n 1531  g 1532  h 1533  g 1534  l 1535  y 1536  l 1537  a 1538   
               
               
                 d 1539  n 1540  l 1541  t 1542  a 1543  d 1544  e 1545  t 1546  k 1547  a 1548  l 1549  k 1550  e 1551  l 1552  y 1553  g 1554  p 1555  v 1556  d 1557   
               
               
                 p 1558  t 1559  f 1560  l 1561  h 1562  r 1563  f 1564  y 1565  s 1566  l 1567  k 1568  a 1569  a 1570  v 1571  h 1572  k 1573  w 1574  k 1575  m 1576   
               
               
                 v 1577  v 1578  c 1579  d 1580  k 1581  v 1582  r 1583  s 1584  l 1585  k 1586  l 1587  s 1588  d 1589  n 1590  n 1591  c 1592  y 1593  l 1594  n 1595   
               
               
                 a 1596  v 1597  i 1598  m 1599  t 1600  l 1601  d 1602  l 1603  l 1604  k 1605  d 1606  i 1607  k 1608  f 1609  v 1610  i 1611  p 1612  a 1613  l 1614   
               
               
                 q 1615  h 1616  a 1617  f 1618  m 1619  k 1620  h 1621  k 1622  g 1623  g 1624  d 1625  g 1626  t 1627  d 1628  f 1629  i 1630  a 1631  l 1632  i 1633   
               
               
                 m 1634  a 1635  y 1636  g 1637  n 1638  c 1639  t 1640  f 1641  g 1642  a 1643  p 1644  d 1645  d 1646  a 1647  s 1648  r 1649  l 1650  l 1651  h 1652   
               
               
                 t 1653  v 1654  l 1655  a 1656  k 1657  a 1658  e 1659  1 1660  c 1661  c 1662  s 1663  a 1664  r 1665  m 1666  v 1667  w 1668  r 1669  e 1670  w 1671   
               
               
                 c 1672  n 1673  v 1674  c 1675  g 1676  i 1677  k 1678  d 1679  v 1680  v 1681  l 1682  q 1683  g 1684  l 1685  k 1686  a 1687  c 1688  c 1689  y 1690   
               
               
                 v 1691  g 1692  v 1693  q 1694  t 1695  v 1696  e 1697  d 1698  l 1699  r 1700  a 1701  r 1702  m 1703  t 1704  y 1705  v 1706  c 1707  q 1708  c 1709   
               
               
                 g 1710  g 1711  e 1712  r 1713  h 1714  r 1715  q 1716  i 1717  v 1718  e 1719  h 1720  t 1721  t 1722  p 1723  w 1724  l 1725  l 1726  l 1727  s 1728   
               
               
                 g 1729  t 1730  p 1731  n 1732  e 1733  k 1734  l 1735  v 1736  t 1737  t 1738  s 1739  t 1740  a 1741  p 1742  d 1743  f 1744  v 1745  a 1746  f 1747   
               
               
                 n 1748  v 1749  f 1750  q 1751  g 1752  i 1753  e 1754  t 1755  a 1756  v 1757  g 1758  j 1759  y 1760  v 1761  h 1762  a 1763  r 1764  l 1765  k 1766   
               
               
                 g 1767  g 1768  l 1769  i 1770  l 1771  k 1772  f 1773  d 1774  s 1775  g 1776  t 1777  v 1778  s 1779  k 1780  t 1781  s 1782  d 1783  w 1784  k 1785   
               
               
                 c 1786  k 1787  v 1788  t 1789  d 1790  v 1791  l 1792  f 1793  s 1794  g 1795  q 1796  k 1797  y 1798  s 1799  s 1800  d 8001  c 1802  n 1803  v 1804   
               
               
                 v 1805  r 1806  y 1807  s 1808  l 1809  d 1810  g 1811  n 1812  f 1813  r 1814  t 1815  e 1816  v 1817  d 1818  p 1819  d 1820  l 1821  s 1822  a 1823   
               
               
                 f 1824  y 1825  v 1826  k 1827  d 1828  g 1829  k 1830  y 1831  f 1832  t 1833  s 1834  e 1835  p 1836  p 1837  v 1838  t 1839  y 1840  s 1841  p 1842   
               
               
                 a 1843  t 1844  i 1845  l 1846  a 1847  g 1848  s 1849  v 1850  y 1851  t 1852  n 1853  s 1854  c 1855  l 1856  v 1857  s 1858  s 1859  d 1860  g 1861   
               
               
                 q 1862  p 1863  g 1864  g 1865  d 1866  a 1867  i 1868  s 1869  l 1870  s 1871  f 1872  n 1873  n 1874  l 1875  l 1876  g 1877  f 1878  d 1879  s 1880   
               
               
                 s 1881  k 1882  p 1883  v 1884  t 1885  k 1886  k 1887  y 1888  t 1889  y 1890  s 1891  f 1892  l 1893  p 1894  k 1895  e 1896  d 1897  g 1898  d 1899   
               
               
                 v 1900  l 1901  l 1902  a 1903  e 1904  f 1905  d 1906  t 1907  y 1908  d 1909  p 1910  i 1911  y 1912  k 1913  n 1914  g 1915  a 1916  m 1917  y 1918   
               
               
                 k 1919  g 1920  k 1921  p 1922  i 1923  l 1924  w 1925  v 1926  n 1927  k 1928  a 1929  s 1930  y 1931  d 1932  t 1933  n 1934  l 1935  n 1936  k 1937   
               
               
                 f 1938  n 1939  r 1940  a 1941  s 1942  l 1943  r 1944  q 1945  i 1946  f 1947  d 1948  v 1949  a 1950  p 1951  i 1952  e 1953  l 1954  e 1955  n 1956   
               
               
                 k 1957  f 1958  t 1959  p 1960  l 1961  s 1962  v 1963  g 1964  s 1965  t 1966  p 1967  v 1968  e 1969  p 1970  p 1971  t 1972  v 1973  d 1974  v 1975   
               
               
                 v 1976  a 1977  l 1978  q 1979  q 1980  e 1981  m 1982  t 1983  i 1984  v 1985  k 1986  c 1987  k 1988  g 1989  l 1990  n 1991  k 1992  p 1993  f 1994   
               
               
                 v 1995  k 1996  d 1997  n 1998  v 1999  s 2000  f 2001  v 2002  a 2003  d 2004  d 2005  s 2006  g 2007  t 2008  p 2009  v 2010  v 2011  e 2012  y 2013   
               
               
                 l 2014  s 2015  k 2016  e 2017  d 2018  l 2019  h 2020  t 2021  l 2022  y 2023  v 2024  d 2025  p 2026  k 2027  y 2028  q 2029  v 2030  i 2031  v 2032   
               
               
                 l 2033  k 2034  d 2035  n 2036  v 2037  l 2038  s 2039  s 2040  m 2041  l 2042  r 2043  l 2044  h 2045  t 2046  v 2047  e 2048  s 2049  g 2050  d 2051   
               
               
                 i 2052  n 2053  v 2054  v 2055  a 2056  a 2057  s 2058  g 2059  s 2060  l 2061  t 2062  r 2063  k 2064  v 2065  k 2066  l 2067  l 2068  f 2069  r 2070   
               
               
                 a 2071  s 2072  f 2073  y 2074  f 2075  k 2076  e 2077  f 2078  a 2079  t 2080  r 2081  t 2082  f 2083  t 2084  a 2085  t 2086  t 2087  a 2088  v 2089   
               
               
                 g 2090  s 2091  c 2092  i 2093  k 2094  s 2095  v 2096  v 2097  r 2098  h 2099  l 2100  g 2101  v 2102  t 2103  k 2104  g 2105  i 2106  l 2107  t 2108   
               
               
                 g 2109  c 2110  f 2111  s 2112  f 2113  v 2114  k 2115  m 2116  l 2117  f 2118  m 2119  l 2120  p 2121  l 2122  a 2123  y 2124  f 2125  s 2126  d 2127   
               
               
                 s 2128  k 2129  l 2130  g 2131  t 2132  t 2133  e 2134  v 2135  k 2136  v 2137  s 2138  a 2139  l 2143  k 2141  t 2142  a 2143  g 2144  v 2145  v 2146   
               
               
                 t 2147  g 2148  n 2149  v 2150  v 2151  k 2152  q 2153  c 2154  c 2155  t 2156  a 2157  a 2158  v 2159  d 2160  l 2161  s 2162  m 2163  d 2164  k 2165   
               
               
                 l 2166  r 2167  r 2168  v 2169  d 2170  w 2171  k 2172  s 2173  t 2174  l 2175  r 2176  l 2177  l 2178  l 2179  m 2180  l 2181  c 2182  t 2183  t 2184   
               
               
                 m 2185  v 2186  l 2187  l 2188  s 2189  s 2190  v 2191  y 2192  h 2193  l 2194  y 2195  v 2196  f 2197  n 2198  q 2199  v 2200  l 2201  s 2202  s 2203   
               
               
                 d 2204  v 2205  m 2206  f 2207  e 2208  d 2209  a 2210  q 2211  g 2212  l 2213  k 2214  k 2215  f 2216  y 2217  k 2218  e 2219  v 2220  r 2221  a 2222   
               
               
                 y 2223  l 2224  g 2225  i 2226  s 2227  s 2228  a 2229  c 2230  d 2231  g 2232  l 2233  a 2234  s 2235  a 2236  y 2237  r 2238  a 2239  n 2240  s 2241   
               
               
                 f 2242  d 2243  v 2244  p 2245  t 2246  f 2247  c 2248  a 2249  n 2250  r 2251  s 2252  a 2253  m 2254  c 2255  n 2256  w 2257  c 2258  l 2259  i 2260   
               
               
                 s 2261  q 2262  d 2263  s 2264  i 2265  t 2266  h 2267  y 2268  p 2269  a 2270  l 2271  k 2272  m 2273  v 2274  q 2275  t 2276  h 2277  l 2278  s 2279   
               
               
                 h 2280  y 2281  v 2282  l 2283  n 2284  i 2285  d 2286  w 2287  l 2288  w 2289  f 2290  a 2291  f 2292  e 2293  t 2294  g 2295  l 2296  a 2297  y 2298   
               
               
                 m 2299  l 2300  y 2301  t 2302  s 2303  a 2304  f 2305  n 2306  w 2307  l 2308  l 2309  l 2310  a 2311  g 2312  t 2313  l 2314  h 2315  y 2316  f 2317   
               
               
                 f 2318  a 2319  q 2320  t 2321  s 2322  i 2323  f 2324  v 2325  d 2326  w 2327  r 2328  s 2329  y 2330  n 2331  y 2332  a 2333  v 2334  s 2335  s 2336   
               
               
                 a 2337  f 2338  w 2339  l 2340  f 2341  t 2342  j 2343  i 2344  p 2345  m 2346  a 2347  g 2348  l 2349  v 2350  r 2351  m 2352  y 2353  n 2354  l 2355   
               
               
                 l 2356  a 2357  c 2358  l 2359  w 2360  l 2361  l 2362  r 2363  k 2364  f 2365  y 2366  q 2367  h 2368  v 2369  i 2370  n 2371  g 2372  c 2373  k 2374   
               
               
                 d 2375  t 2376  a 2377  c 2378  l 2379  l 2380  c 2381  y 2382  k 2383  r 2384  n 2385  r 2386  l 2387  t 2388  r 2389  v 2390  e 2391  a 2392  s 2393   
               
               
                 t 2394  v 2395  v 2396  c 2397  g 2398  g 2399  k 2400  r 2401  t 2402  f 2403  y 2404  i 2405  t 2406  a 2407  n 2408  g 2409  g 2410  i 2411  s 2412   
               
               
                 f 2413  r 2414  r 2415  h 2416  n 2417  n 2418  w 2419  n 2420  c 2421  v 2422  d 2423  c 2424  d 2425  t 2426  a 2427  g 2428  v 2429  g 2430  n 2431   
               
               
                 t 2432  f 2433  i 2434  c 2435  e 2436  e 2437  v 2438  a 2439  n 2440  d 2441  l 2442  t 2443  t 2444  a 2445  l 2446  r 2447  r 2448  p 2449  i 2450   
               
               
                 n 2451  a 2452  t 2453  d 2454  r 2455  s 2456  h 2457  y 2458  y 2459  v 2460  d 2461  s 2462  v 2463  t 2464  v 2465  k 2466  e 2467  t 2468  v 2469   
               
               
                 v 2470  q 2471  f 2472  n 2473  y 2474  r 2475  r 2476  d 2477  g 2478  q 2479  p 2480  f 2481  y 2482  e 2483  r 2484  f 2485  p 2486  l 2487  c 2488   
               
               
                 a 2489  f 2490  t 2491  n 2492  l 2493  d 2494  k 2495  l 2496  k 2497  f 2498  k 2499  e 2500  v 2501  c 2502  k 2503  t 2504  t 2505  t 2506  g 2507   
               
               
                 i 2508  p 2509  e 2510  y 2511  n 2512  f 2513  i 2514  i 2515  y 2516  d 2517  s 2518  s 2519  d 2520  r 2521  g 2522  q 2523  e 2524  s 2525  l 2526   
               
               
                 a 2527  r 2528  s 2529  a 2530  c 2531  v 2532  y 2533  y 2534  s 2535  q 2536  v 2537  l 2538  c 2539  k 2540  s 2541  i 2542  l 2543  l 2544  v 2545   
               
               
                 d 2546  s 2547  s 2548  l 2549  v 2550  t 2551  s 2552  v 2553  g 2554  d 2555  s 2556  s 2557  e 2558  i 2559  a 2560  t 2561  k 2562  m 2563  f 2564   
               
               
                 d 2565  s 2566  f 2567  v 2568  n 2569  s 2570  f 2571  v 2572  s 2573  l 2574  y 2575  n 2576  v 2577  t 2578  r 2579  d 2580  k 2581  l 2582  e 2583   
               
               
                 k 2584  l 2585  i 2586  s 2587  t 2588  a 2589  r 2590  d 2591  v 2593  r 2594  r 2595  g 2596  d 2597  n 2598  f 2599  h 2600  s 2601  v 2602  l 2603   
               
               
                 t 2604  t 2605  f 2606  i 2607  d 2608  a 2609  a 2610  r 2611  g 2612  p 2613  a 2614  g 2615  v 2616  e 2617  s 2618  d 2619  v 2620  e 2621  t 2622   
               
               
                 n 2623  e 2624  i 2625  v 2626  d 2627  s 2628  v 2629  q 2630  y 2631  a 2632  h 2633  k 2634  h 2635  d 2636  i 2637  q 2638  i 2639  t 2640  n 2641   
               
               
                 e 2642  s 2643  y 2644  n 2645  n 2646  y 2647  v 2648  p 2649  s 2630  y 2651  v 2652  k 2653  p 2654  d 2655  s 2656  v 2657  s 2658  t 2659  s 2660   
               
               
                 d 2661  l 2662  g 2663  s 2664  l 2665  i 2666  d 2667  c 2668  n 2669  a 2670  a 2671  s 2672  v 2673  n 2674  q 2675  i 2676  v 2677  l 2678  r 2679   
               
               
                 n 2680  s 2681  n 2682  g 2683  a 2684  c 2685  i 2686  w 2687  n 2688  a 2689  a 2690  a 2691  y 2692  m 2693  k 2694  l 2695  s 2696  d 2697  a 2698   
               
               
                 l 2699  k 2700  r 2701  q 2702  i 2703  r 2704  i 2705  a 2706  c 2707  r 2708  k 2709  c 2710  n 2711  l 2712  a 2713  f 2714  r 2715  l 2716  t 2717   
               
               
                 t 2718  s 2719  k 2720  l 2721  r 2722  a 2723  n 2724  d 2725  n 2726  i 2727  l 2728  s 2729  v 2730  r 2731  f 2732  t 2733  a 2734  n 2735  k 2736   
               
               
                 i 2737  v 2738  g 2739  g 2740  a 2741  p 2742  t 2743  w 2744  f 2745  n 2746  a 2747  l 2748  r 2749  d 2750  f 2751  t 2752  l 2753  k 2754  g 2755   
               
               
                 y 2756  v 2757  l 2758  a 2759  t 2760  i 2761  i 2762  v 2763  f 2764  l 2765  c 2766  a 2767  v 2768  l 2769  m 2770  y 2771  l 2772  c 2773  l 2774   
               
               
                 p 2775  t 2776  f 2777  s 2778  m 2779  v 2780  p 2781  v 2782  e 2783  f 2784  y 2785  e 2786  d 2787  r 2788  i 2789  l 2790  d 2791  f 2792  k 2793   
               
               
                 v 2794  l 2795  d 2796  n 2797  g 2798  i 2799  i 2800  r 2801  d 2802  v 2803  n 2804  p 2805  d 2806  d 2807  k 2808  c 2809  f 2810  a 2811  n 2812   
               
               
                 k 2813  h 2814  r 2815  s 2816  f 2817  t 2818  q 2819  w 2820  y 2821  h 2822  e 2823  h 2824  v 2825  g 2826  g 2827  v 2828  y 2829  d 2830  n 2831   
               
               
                 s 2832  i 2833  t 2834  c 2835  p 2836  l 2837  t 2838  v 2839  a 2840  v 2841  i 2842  a 2843  g 2844  v 2845  a 2846  g 2847  a 2848  r 2849  i 2850   
               
               
                 p 2851  d 2852  v 2853  p 2854  t 2855  t 2856  l 2857  a 2858  w 2859  v 2860  n 2861  n 2862  q 2863  i 2864  i 2865  f 2866  f 2867  v 2868  s 2869   
               
               
                 r 2870  v 2871  f 2872  a 2873  n 2874  t 2875  g 2876  s 2877  v 2878  c 2879  y 2880  t 2881  p 2882  i 2883  d 2884  e 2885  i 2886  p 2887  y 2888   
               
               
                 k 2889  s 2890  f 2891  s 2892  d 2893  s 2894  g 2895  c 2896  i 2897  l 2898  p 2899  s 2900  e 2901  c 2902  t 2903  m 2904  f 2905  r 2906  d 2907   
               
               
                 a 2908  e 2909  g 2910  r 2911  m 2912  t 2913  p 2914  y 2915  c 2916  h 2917  d 2918  p 2919  t 2920  v 2921  l 2922  p 2923  g 2924  a 2925  f 2926   
               
               
                 a 2927  y 2928  s 2929  q 2930  m 2931  r 2932  p 2933  h 2934  v 2935  r 2936  y 2937  d 2938  l 2939  y 2940  d 2941  g 2942  n 2943  m 2944  f 2945   
               
               
                 i 2946  k 2947  f 2948  p 2949  e 2950  v 2951  v 2952  f 2953  e 2954  s 2955  t 2956  l 2957  r 2958  i 2959  t 2960  r 2961  t 2962  l 2963  s 2964   
               
               
                 t 2965  q 2966  y 2967  c 2968  r 2969  f 2970  g 2971  s 2972  c 2973  e 2974  y 2975  a 2976  q 2977  e 2978  g 2979  v 2980  c 2981  i 2982  t 2983   
               
               
                 t 2984  n 2985  g 2986  s 2987  w 2988  a 2989  i 2990  f 2991  n 2992  d 2993  h 2994  h 2995  l 2996  n 2997  r 2998  p 2999  g 3000  v 3001  y 3002   
               
               
                 c 3003  g 3004  s 3005  d 3006  f 3007  i 3008  d 3009  i 3010  v 3011  r 3012  r 3013  l 3014  a 3015  v 3016  s 3017  l 3018  f 3019  q 3020  p 3021   
               
               
                 i 3022  t 3023  y 3024  f 3025  q 3026  l 3027  t 3028  t 3029  s 3030  l 3031  v 3032  l 3033  g 3034  i 3035  g 3036  l 3037  c 3038  a 3039  f 3040   
               
               
                 l 3041  t 3042  l 3043  l 3044  f 3045  y 3046  y 3047  i 3048  n 3049  k 3050  v 3051  k 3052  r 3053  a 3054  f 3055  a 3056  d 3057  y 3058  t 3059   
               
               
                 q 3060  c 3061  a 3062  v 3063  i 3064  a 3065  v 3066  v 3067  a 3068  a 3069  v 3070  l 3071  n 3072  s 3073  l 3074  c 3075  i 3076  c 3077  f 3078   
               
               
                 v 3079  a 3080  s 3081  i 3082  p 3083  l 3084  c 3085  i 3086  v 3087  p 3088  y 3089  t 3090  a 3091  l 3092  y 3093  y 3094  y 3095  a 3096  t 3097   
               
               
                 f 3098  y 3099  f 3100  t 3101  n 3102  e 3103  p 3104  a 3105  f 3106  i 3107  m 3108  h 3109  v 3110  s 3111  w 3112  y 3113  i 3114  m 3115  f 3116   
               
               
                 g 3117  p 3118  i 3119  v 3120  p 3121  i 3122  w 3123  m 3124  t 3125  c 3126  v 3127  y 3128  t 3129  v 3130  a 3131  m 3132  c 3133  f 3134  r 3135   
               
               
                 h 3136  f 3137  f 3138  w 3139  v 3140  l 3141  a 3142  y 3143  f 3144  s 3145  k 3146  k 3147  h 3148  v 3149  e 3150  v 3151  f 3152  t 3153  d 3154   
               
               
                 g 3155  k 3156  l 3157  n 3158  c 3159  s 3160  f 3161  q 3162  d 3163  a 3164  a 3165  s 3166  n 3167  i 3168  f 3169  v 3170  i 3171  n 3172  k 3173   
               
               
                 d 3174  t 3175  y 3176  a 3177  a 3178  l 3179  r 3180  n 3181  s 3182  l 3183  t 3184  n 3185  d 3186  a 3187  y 3188  s 3189  r 3190  f 3191  l 3192   
               
               
                 g 3193  l 3194  f 3195  n 3196  k 3197  y 3198  k 3199  y 3200  f 3201  s 3202  g 3203  a 3204  m 3205  e 3206  t 3207  a 3208  a 3209  y 3210  r 3211   
               
               
                 e 3212  a 3213  a 3214  a 3215  c 3216  h 3217  l 3218  a 3219  k 3220  a 3221  l 3222  q 3223  t 3224  y 3225  s 3226  e 3227  t 3228  g 3229  5 3230   
               
               
                 d 3231  l 3232  l 3233  y 3234  q 3235  p 3236  p 3237  n 3238  c 3239  s 3240  i 3241  t 3242  s 3243  g 3244  v 3245  l 3246  q 3247  s 3248  g 3249   
               
               
                 l 3250  v 3251  k 3252  m 3253  s 3254  h 3255  p 3256  s 3257  g 3258  d 3259  v 3260  e 3261  a 3262  c 3263  m 3264  v 3265  q 3266  v 3267  t 3268   
               
               
                 c 3269  g 3270  s 3271  m 3272  t 3273  l 3274  n 3275  g 3276  l 3277  w 3278  l 3279  d 3280  n 3281  t 3282  v 3283  w 3284  c 3285  p 3286  r 3287   
               
               
                 h 3288  v 3289  m 3290  c 3291  p 3292  a 3293  d 3294  q 3295  l 3296  s 3297  d 3298  p 3299  n 3300  y 3301  d 3302  a 3303  l 3304  l 3305  i 3306   
               
               
                 s 3307  m 3308  t 3309  n 3310  h 3311  s 3312  f 3313  s 3314  v 3315  q 3316  k 3317  h 3318  i 3319  g 3320  a 3321  p 3322  a 3323  n 3324  l 3325   
               
               
                 r 3326  v 3327  v 3328  g 3329  h 3330  a 3331  m 3332  q 3333  g 3334  t 3335  l 3336  l 3337  k 3338  l 3339  t 3340  v 3341  d 3342  v 3343  a 3344   
               
               
                 n 3345  p 3346  s 3347  t 3348  p 3349  a 3350  y 3351  t 3352  f 3353  t 3354  t 3355  v 3356  k 3357  p 3358  g 3359  a 3360  a 3361  f 3362  s 3363   
               
               
                 v 3364  l 3365  a 3366  c 3367  y 3368  n 3369  g 3370  r 3371  p 3372  t 3373  g 3374  t 3375  f 3376  t 3377  v 3378  v 3379  m 3380  r 3381  p 3382   
               
               
                 n 3383  y 3384  t 3385  i 3386  k 3387  g 3388  s 3389  f 3390  l 3391  c 3392  g 3393  s 3394  c 3395  g 3396  s 3397  v 3398  g 3399  y 3400  t 3401   
               
               
                 k 3402  e 3403  g 3404  s 3405  v 3406  i 3407  n 3408  f 3409  c 3410  y 3411  m 3412  h 3413  q 3414  m 3415  e 3416  l 3417  a 3418  n 3419  g 3420   
               
               
                 t 3421  h 3422  t 3423  g 3424  s 3425  a 3426  f 3427  d 3428  g 3429  t 3430  m 3431   y 3432  g 3433  a 3434  f 3435  m 3436  d 3437  k 3438  q 3439   
               
               
                 v 3440  h 3441  q 3442  v 3443  q 3444  l 3445  t 3446  d 3447  k 3448  y 3449  c 3450  s 3451  v 3452  n 3453  v 3454  v 3455  a 3456  w 3457  l 3458   
               
               
                 y 3459  a 3460  a 3461  i 3462  l 3463  n 3464  g 3465  c 3466  a 3467  w 3468  f 3469  v 3470  k 3471  p 3472  n 3473  r 3474  t 3475  s 3476  v 3477   
               
               
                 v 3478  s 3479  f 3480  n 3481  e 3482  w 3483  a 3484  l 3485  a 3486  n 3487  q 3488  f 3489  t 3490  e 3491  f 3492  v 3493  g 3494  t 3495  q 3496   
               
               
                 s 3497  v 3498  d 3499  m 3500  l 3501  a 3502  v 3503  k 3504  t 3505  g 3506  v 3507  a 3508  i 3509  e 3510  q 3511  l 3512  l 3513  y 3514  a 3515   
               
               
                 i 3516  q 3517  q 3518  l 3519  y 3520  t 3521  g 3522  f 3523  q 3524  g 3525  k 3526  q 3527  i 3528  l 3529  g 3530  s 3531  t 3532  m 3533  l 3534   
               
               
                 e 3535  d 3536  e 3537  f 3538  t 3539  p 3540  e 3541  d 3542  v 3543  n 3544  m 3545  q 3546  i 3547  m 3548  g 3549  v 3550  v 3551  m 3552  q 3553   
               
               
                 s 3554  g 3555  v 3556  r 3557  k 3558  v 3559  t 3560  y 3561  g 3562  t 3563  a 3564  h 3565  w 3566  l 3567  f 3568  a 3569  t 3570  l 3571  v 3572   
               
               
                 s 3573  t 3574  y 3575  v 3576  i 3577  i 3578  l 3579  q 3580  a 3581  t 3582  k 3583  f 3584  t 3585  l 3586  w 3587  n 3588  y 3589  l 3590  f 3591   
               
               
                 e 3592  t 3593  i 3594  p 3595  t 3596  q 3597  l 3598  f 3599  p 3600  l 3601  l 3602  f 3603  v 3604  t 3605  m 3606  a 3607  f 3608  v 3609  m 3610   
               
               
                 l 3611  l 3612  v 3613  k 3614  h 3615  k 3616  h 3617  t 3618  f 3619  l 3620  t 3621  l 3622  f 3623  l 3624  l 3625  p 3626  v 3627  a 3628  i 3629   
               
               
                 c 3630  l 3631  t 3632  y 3633  a 3634  n 3635  i 3636  v 3637  y 3638  e 3639  p 3640  t 3641  t 3642  p 3643  i 3644  s 3645  s 3646  a 3647  l 3648   
               
               
                 i 3649  a 3650  v 3651  a 3652  n 3653  w 3654  l 3655  a 3656  p 3657  t 3658  a 3659  a 3660  y 3661  m 3662  r 3663  t 3664  t 3665  h 3666  t 3667   
               
               
                 d 3668  i 3669  g 3670  v 3671  y 3672  i 3673  s 3674  m 3675  s 3676  l 3677  v 3678  l 3679  v 3680  i 3681  v 3682  v 3683  k 3684  r 3685  l 3686   
               
               
                 y 3687  n 3688  p 3689  s 3690  l 3691  s 3692  n 3693  f 3694  a 3695  l 3696  a 3697  l 3698  c 3699  s 3700  g 3701  v 3702  m 3703  w 3704  l 3705   
               
               
                 y 3706  t 3707  y 3708  s 3709  i 3710  g 3711  e 3712  a 3713  s 3714  s 3715  p 3716  i 3717  a 3718  y 3719  l 3720  v 3721  f 3722  v 3723  t 3724   
               
               
                 t 3725  l 3726  t 3727  s 3728  d 3729  y 3730  t 3731  i 3732  t 3733  v 3734  f 3735  v 3736  t 3737  v 3738  n 3739  l 3740  a 3741  k 3742  v 3743   
               
               
                 c 3744  t 3745  y 3746  a 3747  i 3748  f 3749  a 3750  y 3751  s 3752  p 3753  q 3754  l 3755  t 3756  l 3757  v 3758  f 3759  p 3760  e 3761  v 3762   
               
               
                 k 3763  m 3764  i 3765  l 3766  l 3767  l 3768  y 3769  t 3770  c 3771  l 3772  g 3773  f 3774  m 3775  c 3776  t 3777  c 3778  y 3779  f 3780  g 3781   
               
               
                 v 3782  f 3783  s 3784  l 3785  l 3786  n 3787  l 3788  k 3789  l 3790  r 3791  a 3792  p 3793  m 3794  g 3795  v 3796  y 3797  d 3798  f 3799  k 3800   
               
               
                 v 3801  s 3802  t 3803  q 3804  e 3805  f 3806  r 3807  f 3808  m 3809  t 3810  a 3811  n 3812  n 3813  l 3814  t 3815  a 3816  p 3817  r 3818  n 3819   
               
               
                 s 3820  w 3821  e 3822  a 3823  m 3824  a 3825  l 3826  n 3827  f 3828  k 3829  l 3830  i 3831  g3 832  i 3833  g 3834  g 3835  t 3836  p 3837  c 3838   
               
               
                 i 3839  k 3840  v 3841  a 3842  a 3843  m 3844  q 3845  s 3846  k 3847  l 3848  t 3849  d 3850  l 3851  k 3852  c 3853  t 3854  s 3855  v 3856  v 3857   
               
               
                 l 3858  l 3859  s 3860  v 3861  l 3862  q 3863  q 3864  l 3865  h 3866  l 3867  e 3868  a 3869  n 3870  s 3871  r 3872  a 3873  w 3874  a 3875  f 3876   
               
               
                 c 3877  v 3878  k 3879  c 3880  h 3881  n 3882  d 3883  i 3884  l 3885  a 3886  a 3887  t 3888  d 3889  p 3890  s 3891  e 3892  a 3893  f 3894  e 3895   
               
               
                 k 3896  f 3897  v 3898  s 3899  l 3900  f 3901  a 3902  t 3903  l 3904  m 3905  t 3906  f 3907  s 3908  g 3909  a 3910  v 3911  d 3912  l 3913  d 3914   
               
               
                 a 3915  l 3916  a 3917  s 3918  d 3919  i 3920  f 3921  d 3922  t 3923  p 3924  s 3925  v 3926  l 3927  q 3928  a 3929  t 3930  l 3931  s 3932  e 3933   
               
               
                 f 3934  s 3935  h 3936  l 3937  a 3938  t 3939  f 3940  a 3941  e 3942  l 3943  e 3944  a 3945  a 3946  q 3947  k 3948  a 3949  y 3950  q 3951  e 3952   
               
               
                 a 3953  m 3954  d 3955  s 3956  g 3957  d 3958  t 3959  s 3960  p 3961  q 3962  v 3963  l 3964  k 3965  a 3966  l 3967  q 3968  k 3969  a 3970  v 3971   
               
               
                 n 3972  i 3973  a 3974  k 3975  n 3976  a 3977  y 3978  e 3979  k 3980  d 3981  k 3982  a 3983  v 3984  a 3985  r 3986  k 3987  l 3988  e 3989  r 3990   
               
               
                 m 3991  a 3992  d 3993  q 3994  a 3995  m 3996  t 3997  s 3998  m 3999  y 4000  k 4001  q 4002  a 4003  r 4004  a 4005  e 4006  d 4007  k 4008  k 4009   
               
               
                 a 4010  k 4011  i 4012  v 4013  s 4014  a 4015  m 4016  q 4017  t 4018  m 4019  l 4020  f 4021  g 4022  m 4023  i 4024  k 4025  k 4026  l 4027  d 4028   
               
               
                 n 4029  d 4030  v 4031  l 4032  n 4033  g 4034  i 4035  i 4036  s 4037  n 4038  a 4039  r 4040  n 4041  g 4042  c 4043  i 4044  p 4045  l 4046  s 4047   
               
               
                 v 4048  i 4049  p 4050  l 4051  c 4052  a 4053  s 4054  n 4055  k 4056  l 4057  r 4058  v 4059  v 4060  i 4061  p 4062  d 4063  f 4064  t 4065  v 4066   
               
               
                 w 4067  n 4068  q 4069  v 4070  v 4071  t 4072  y 4073  p 4074  s 4075  l 4076  n 4077  y 4078  a 4079  g 4080  a 4081  l 4082  w 4083  d 4084  i 4085   
               
               
                 t 4086  v 4087  i 4088  n 4089  n 4090  v 4091  d 4092  n 4093  e 4094  i 4095  v 4096  k 4097  s 4098  s 4099  d 4100  v 4101  v 4102  d 4103  s 4104   
               
               
                 n 4105  e 4106  n 4107  l 4108  t 4109  w 4110  p 4111  l 4112  v 4113  l 4114  e 4115  c 4116  t 4117  r 4118  a 4119  d 4120  t 4121  s 4122  a 4123   
               
               
                 v 4124  k 4125  l 4126  q 4127  n 4128  n 4129  e 4130  i 4131  k 4132  p 4133  s 4134  g 4135  l 4136  k 4137  t 4138  m 4139  v 4140  v 4141  s 4142   
               
               
                 a 4143  g 4144  q 4145  e 4146  q 4147  t 4148  n 4149  c 4150  n 4151  t 4152  s 4153  s 4154  l 4155  a 4156  y 4157  y 4158  e 4159  p 4160  v 4161   
               
               
                 q 4162  g 4163  r 4164  k 4165  m 4166  l 4167  m 4168  a 4169  l 4170  l 4171  s 4172  d 4173  n 4174  a 4175  y 4176  l 4177  k 4178  w 4179  a 4180   
               
               
                 r 4181  v 4182  e 4183  g 4184  k 4185  d 4186  g 4187  f 4188  v 4189  s 4190  v 4191  e 4192  l 4193  q 4194  p 4195  p 4196  c 4197  k 4198  f 4199   
               
               
                 l 4200  i 4201  a 4202  g 4203  p 4204  k 4205  g 4206  p 4207  e 4208  i 4209  r 4210  y 4211  l 4212  y 4213  f 4214  v 4215  k 4216  n 4217  l 4218   
               
               
                 n 4219  n 4220  l 4221  h 4222  r 4223  g 4224  q 4225  v 4226  l 4227  g 4228  h 4229  i 4230  a 4231  a 4232  t 4233  v 4234  r 4235  l 4236  q 4237   
               
               
                 a 4238  g 4239  s 4240  n 4241  t 4242  e 4243  f 4244 a 4245  s 4246  n 4247  s 4248  s 4249  v 4250  l 4251  s 4252  l 4253  v 4254  n 4255  f 4256   
               
               
                 t 4257  v 4258  d 4259  p 4260  q 4261  k 4262  a 4263  y 4264  l 4265  d 4266  f 4267  v 4268  n 4269  a 4270  g 4271  g 4272  a 4273  p 4274  l 4275   
               
               
                 t 4276  n 4277  c 4278  v 4279  k 4280  m 4281  l 4282  t 4283  p 4284  k 4285  t 4286  g 4287  t 4288  g 4289  i 4290  a 4291  i 4292  s 4293  v 4294   
               
               
                 k 4295  p 4296  e 4297  s 4298  t 4299  a 4300  d 4301  q 4302  e 4303  t 4304  y 4305  g 4306  g 4307  a 4308  s 4309  v 4310  c 4311  l 4312  y 4313   
               
               
                 c 4314  r 4315  a 4316  h 4317  i 4318  e 4319  h 4320  p 4321  d 4322  v 4323  s 4324  g 4325  v 4326  c 4327  k 4328  y 4329  k 4330  g 4331  k 4332   
               
               
                 f 4333  v 4334  q 4335  i 4336  p 4337  a 4338  q 4339  c 4340  v 4341  r 4342  d 4343  p 4344  v 4345  g 4346  f 4347  c 4348  l 4349  s 4350  n 4351   
               
               
                 t 4352  p 4353  c 4354  n 4355  v 4356  c 4357  q 4358  y 4359  w 4360  i 4361  g 4362  y 4363  g 4364  c 4365  n 4366  c 4367  d 4368  s 4369  l 4370   
               
               
                 r 4371  q 4372  a 4373  a 4374  l 4375  p 4376  q 4377  s 4378  k 4379  d 4380  s 4381  n 4382  f 4383  l 4384  n 4385  r 4386  v 4387  r 4388  g 4389   
               
               
                 s 4390  i 4391  v 4392  n 4393  a 4394  r 4395  i 4396  e 4397  p 4398  c 4399  s 4400  s 4401  g 4402  l 4403  s 4404  t 4405  d 4406  v 4407  v 4408   
               
               
                 f 4409  r 4410  a 4411  f 4412  d 4413  i 4414  c 4415  n 4416  y 4417  k 4418  a 4419  k 4420  v 4421  a 4422  g 4423  i 4424  g 4425  k 4426  y 4427   
               
               
                 y 4428  k 4429  t 4430  n 4431  t 4432  c 4433  r 4434  f 1435  v 4436  e 4437  l 4438  d 4439  d 4440  q 4441  g 4442  h 4443  h 4444  l 4445  d 4446   
               
               
                 s 4447  y 4448  f 4449  v 4450  v 4451  k 4452 r 4453  h 4454  t 4455  m 4456  e 4457  n 4458  y 4459  e 4460  l 4461  e 4462  k 4463  h 4464  c 4465   
               
               
                 y 4466  d 4467  l 4468  l 4469  r 4470  d 4471  c 4472  d 4473  a 4474  v 4475  a 4476  p 4477  h 4478  d 4479  f 4480  f 4481  i 4482  f 4483  d 4484   
               
               
                 v 4485  d 4486  k 4487  v 4488  k 4489  t 4490  p 4491  h 4492  i 4493  v 4494  r 4495  q 4496  r 4497  l 4498  t 4499  e 4500  y 4501  t 4502  m 4503   
               
               
                 m 4504  d 4505  l 4506  v 4507  y 4508  a 4509  l 4510  r 4511  h 4512  f 4513  d 4514  q 4515  n 4516  s 4517  e 4518  v 4519  l 4520  k 4521  a 4522   
               
               
                 i 4523  l 4524  v 4525  k 4526  y 4527  g 4528  c 4529  c 4530  d 4531  v 4532  t 4533  y 4534  f 4535  e 4536  n 4537  k 4538  l 4539  w 4540  f 4541   
               
               
                 d 4542  f 4543  v 4544  e 4545  n 4546  p 4547  s 4548  v 4549  i 4550  g 4551  v 4552  y 4553  j 4554  k 4555  l 4556  g 4557  e 4558  r 4559  v 4560   
               
               
                 r 4561  q 4562  a 4563  i 4564  l 4565  n 4566  t 4567  v 4568  k 4569  f 4570  c 4571  d 4572  h 4573  m 4574  v 4575  k 4576  a 4577  g 4578  l 4579   
               
               
                 v 4580  g 4581  v 4582  l 4583  t 4584  l 4585  d 4586  n 4587  g 4588  d 4589  l 4590  n 4591  g 4592  k 4593  w 4594  y 4595  d 4596  f 4597  g 4598   
               
               
                 d 4599  f 4600  v 4601  i 4602  t 4603  q 4604  p 4605  g 4606  s 4607  g 4608  v 4609  a 4610  i 4611  v 4612  d 4613  s 4614  y 4615  y 4616  s 4617   
               
               
                 y 4618  l 4619  m 4620  p 4621  v 4622  l 4623  s 4624  m 4625  t 4626  d 4627  c 4628  l 4629  a 4630  a 4631  e 4632  t 4633  h 4634  r 4635  d 4636   
               
               
                 c 4637  d 4638  f 4639  n 4640  k 4641  p 4642  l 4643  i 4614  e 4645  w 4646  p 4647  l 4648  t 4649  e 4650  y 4651  d 4652  f 4653  t 4654  d 4655   
               
               
                 y 4656  k 4657  v 4658  q 4659  l 4660  f 4661  e 4662  k 4663  y 4664  f 4665  k 4666  y 4667  w 4668  d 4669  q 4670  t 4671  y 4672  h 4673  a 4674   
               
               
                 n 4675  c 4676  v 4677  n 4678  c 4679  t 4680  d 4681  d 4682  r 4683  c 4684  v 4685  l 4686  h 4687  c 4688  a 4689  n 4690  f 4691  n 4692  v 4693   
               
               
                 l 4694  f 4695  a 4696  m 4697  t 4698  m 4699  p 4700  k 4701  t 4702  c 4703  f 4704  g 4705  p 4706  i 4707  v 4708  r 4709  k 4710  i 4711  f 4712   
               
               
                 v 4713  d 4714  g 4715  v 4716  p 4717  f 4718  v 4719  v 4720  s 4721  c 4722  g 4723  y 4724  h 4725  y 4726  k 4727  e 4728  l 4729  g 4730  l 4731   
               
               
                 v 4732  m 4733  n 4734  m 4735  d 4736  v 4737  s 4738  l 4739  h 4740  r 4741  h 4742  r 4743  l 4744  s 4745  l 4746  k 4747  e 4748  l 4749  m 4750   
               
               
                 m 4751  y 4752  a 4753  a 4754  d 4755  p 4756  a 4757  m 4758  h 4759  i 4760  a 4761  s 4762  s 4763  n 4764  a 4765  f 4766  l 4767  d 4768  l 4769   
               
               
                 r 4770  t 4771  s 4772  c 4773  f 4774  s 4775  v 4776  a 4777  a 4778  l 4779  t 4780  t 4781  g 4782  l 4783  t 4784  f 4785  q 4786  t 4787  v 4788   
               
               
                 r 4789  p 4790  g 4791  n 4792  f 4793  n 4794  q 4795  d 4796  f 4797  y 4798  d 4799  f 4800  v 4801  v 4802  s 4803  k 4804  g 4805  f 4806  f 4807   
               
               
                 k 4808  e 4809  g 4810  s 4811  s 4812  v 4813  t 4814  l 4815  k 4816  h 4817  f 4818  f 4819  f 4820  a 4821  q 4822  d 4823  g 4824  n 4825  a 4826   
               
               
                 a 4827  i 4828  t 4829  d 4830  y 4831  n 4832  y 4833  y 4834  s 4835  y 4836  n 4837  l 4838  p 4839  t 4840  m 4941  c 4842  d 4943  i 4844  k 4845   
               
               
                 q 4846  m 4847  l 4848  f 4849  c 4850  m 4851  e 4852  v 4853  v 4854  n 4855  k 4856  y 4857  f 4858  e 4859  i 4860  y 4861  d 4862  g 4863  g 4864   
               
               
                 c 4865  l 4866  n 4867  a 4868  s 4869  e 4870  v 4871  v 4872  v 4873  n 4874  n 4875  l 4876  d 4877  k 4878  s 4879  a 4880  g 4881  h 4882  p 4883   
               
               
                 f 4884  n 4885  k 4886  f 4887  g 4888  k 4889  a 4890  r 4891  v 4892  y 4893  y 4894  e 4895  s 4896  m 4897  s 4898  y 4899  q 4900  e 4901  q 4902   
               
               
                 d 4903  e 4904  l 4905  f 4906  a 4907  m 4908  t 4909  k 4910  r 4911  n 4912  v 4913  i 4914  p 4915  t 4916  m 4917  t 4918  q 4919  m 4920  n 4921   
               
               
                 l 4922  k 4923  y 4924  a 4925  i 4926  s 4927  a 4928  k 4929  n 4930  r 4931  a 4932  r 4933  t 4934  v 4935  a 4936  g 4937  v 4938  s 4939  i 4940   
               
               
                 l 4941  s 4942  t 4943  m 4944  t 4945  n 4946  r 4947  q 4948  y 4949  h 4950  q 4951  k 4952  m 4953  l 4954  k 4955  s 4956  m 4957  a 4958  a 4959   
               
               
                 t 4960  r 4961  g 4962  a 4963  t 4964  c 4965  v 4966  i 4967  g 4968  t 4969  t 4970  k 4971  f 4972  y 4973  g 4974  g 4975  w 4976  d 4977  f 4978   
               
               
                 m 4979  l 4980  k 4981  t 4982  l 4983  y 4984  k 4985  d 4986  v 4987  d 4988  n 4989  p 4990  h 4991  l 4992  m 4993  g 4994  w 4995  d 4996  y 4997   
               
               
                 p 4998  k 4999  c 5000  d 5001  r 5002  a 5003  m 5004  p 5005  n 5006  m 5007  c 5008  r 5009  i 5010  f 5011  a 5012  s 5013  l 5014  i 5015  l 5016   
               
               
                 a 5017  r 5018  k 5019  h 5020  g 5021  t 5022  c 5023  c 5024  t 5025  t 5026  r 5027  d 5028  r 5029  f 5030  y 5031  r 5032  l 5033  a 5034  n 5035   
               
               
                 e 5036  c 5037  a 5038  q 5039  v 5040  l 5041  s 5042  e 5043  y 5044  v 5045  l 5046  c 5047  g 5048  g 5049  g 5050  y 5051  y 5052  v 5053  k 5054   
               
               
                 p 5055  g 5056  g 5057  t 5058  s 5059  s 5060  g 5061  d 5062  a 5063  t 5064  t 5065  a 5066  y 5067  a 5068  n 5069  s 5070  v 5071  f 5072  n 5073   
               
               
                 i 5074  l 5075  q 5076  a 5077  t 5078  t 5079  a 5080  n 5081  v 5082  s 5083  a 5084  l 5085  m 5086  g 5087  a 5088  n 5089  g 5090  n 5091  k 5092   
               
               
                 i 5093  v 5094  d 5095  k 5096  e 5097  v 5098  k 5099  d 5100  m 5101  q 5102  f 5103  d 5104  l 5105  y 5106  v 5107  n 5108  v 5109  y 5110  r 5111   
               
               
                 s 5112  t 5113  s 5114  p 5115  d 5116  p 5117  k 5118  f 5119  v 5120  d 5121  k 5122  y 5123  y 5124  a 5125  f 5126  l 5127  n 5128  k 5129  h 5130   
               
               
                 f 5131  s 5132  m 5133  m 5134  i 5135  l 5136  s 5137  d 5138  d 5139  g 5140  v 5141  v 5142  c 5143  y 5144  n 5145  s 5146  d 5147  y 5148  a 5149   
               
               
                 a 5150  k 5151  g 5152  y 5153  i 5154  f 5155  g 5156  i 5157  q 5158  n 5159  f 5160  k 5161  e 5162  t 5163  l 5164  y 5165  y 5166  q 5167  n 5168   
               
               
                 n 5169  v 5170  f 5171  m 5172  s 5173  e 5174  a 5175  k 5176  c 5177  w 5178  v 5179  e 5180  t 5181  d 5182  l 5183  k 5184  k 5185  g 5186  p 5187   
               
               
                 h 5188  e 5189  f 5190  c 5191  s 5192  q 5193  h 5194  t 5195  l 5196  y 5197  i 5198  k 5199  d 5200  g 5201  d 5202  d 5203  g 5204  y 5205  f 5206   
               
               
                 l 5207  p 5208  y 5209  p 5210  d 5211  p 5212  s 5213  r 5214  i 5215  l 5216  s 5217  a 5218  g 5219  c 5220  f 5221  v 5222  d 5223  d 5224  i 5225   
               
               
                 v 5226  k 5227  t 5228  d 5229  g 5230  t 5231  l 5232  m 5233  v 5234  e 5235  r 5236  f 5237  v 5238  s 5239  l 5240  a 5241  i 5242  d 5243  a 5244   
               
               
                 y 5245  p 5246  l 5247  t 5248  k 5249  h 5250  e 5251  d 5252  i 5253  e 5254  y 5255  q 5256  n 5257  v 5258  f 5259  w 5260  v 5261  y 5262  l 5263   
               
               
                 q 5264  y 5265  i 5266  e 5267  k 5268  l 5269  y 5270  k 5271  d 5272  l 5273  t 5274  g 5275  h 5276  m 5277  l 5278  d 5279  s 5280  y 5281  s 5282   
               
               
                 v 5283  m 5284  l 5285  c 5286  g 5287  d 5288  n 5289  s 5290  a 5291  k 5292  f 5293  w 5294  e 5295  e 5296  a 5297  f 5298  y 5299  r 5300  d 5301   
               
               
                 l 5302  y 5303  s 5304  s 5305  p 5306  t 5307  t 5308  l 5309  q 5310  a 5311  v 5312  g 5313  s 5314  c 5315  v 5316  v 5317  c 5318  h 5319  s 5320   
               
               
                 q 5321  t 5322  s 5323  l 5324  r 5325  c 5326  g 5327  t 5328  c 5329  i 5330  r 5331  r 5332  p 5333  f 5334  l 5335  c 5336  c 5337  k 5338  c 5339   
               
               
                 c 5340  y 5341  d 5342  h 5343  v 5344  i 5345  a 5346  t 5347  p 5348  h 5349  k 5350  m 5351  v 5352  l 5353  s 5354  v 5355  s 5356  p 5357  y 5358   
               
               
                 v 5359  c 5360  n 5361  a 5362  p 5363  g 5364  c 5365  g 5366  v 5367  s 5368  d 5369  v 5370  t 5371  k 5372  l 5373  y 5374  l 5375  g 5376  g 5377   
               
               
                 m 5378  s 5379  y 5380  f 5381  c 5382  v 5383  d 5384  h 5385  r 5386  p 5387  v 5388  c 5389  s 5390  f 5391  p 5392  l 5393  c 5394  a 5395  n 5396   
               
               
                 g 5397  l 5398  v 5399  f 5400  g 5401  l 5402  y 5403  k 5404  n 5405  m 5406  c 5407  t 5408  g 5409  s 5410  p 5411  s 5412  i 5413  v 5414  e 5415   
               
               
                 f 5416  n 5417  r 5418  l 5419  a 5420  t 5421  c 5422  d 5423  w 5424  t 5425  e 5426  s 5427  g 5428  d 5429  y 5430  t 5431  l 5432  a 5433  n 5434   
               
               
                 t 5435  t 5436  t 5437  e 5438  p 5439  l 5440  k 5441  l 5442  f 5443  a 5444  a 5445  e 5446  t 5447  l 5448  r 5449  a 5450  t 5451  e 5452  e 5453   
               
               
                 a 5454  s 5455  k 5456  q 5457  s 5458  y 5459  a 5460  i 5461  a 5462  t 5463  i 5464  k 5465  e 5466  i 5467  v 5468  g 5469  e 5470  r 5471  q 5472   
               
               
                 l 5473  l 5474  l 5475  v 5476  w 5477  e 5478  a 5479  g 5480  k 5481  s 5482  k 5483  p 5484  p 5485  l 5486  n 5487  r 5488  n 5489  y 5490  v 5491   
               
               
                 f 5492  t 5493  g 5494  y 5495  h 5496  i 5497  t 5498  k 5499  n 5500  s 5501  k 5502  v 5503  q 5504  l 5505  g 5506  e 5507  y 5508  i 5509  f 5510   
               
               
                 e 5511  r 5512  i 5513  d 5514  y 5515  s 5516  d 5517  a 5518  v 5519  s 5520  y 5521  k 5522  s 5523  s 5524  t 5525  t 5526  y 5527  k 5528  l 5529   
               
               
                 t 5530  v 5531  g 5532  d 5533  i 5534  f 5535  v 5536  l 5537  t 5538  s 5539  h 5540  s 5541  v 5542  a 5543  t 5544  l 5545  t 5546  a 5547  p 5548   
               
               
                 t 5549  i 5550  v 5551  n 5552  q 5553  e 5554  r 5555  y 5556  v 5557  k 5558  i 5559  t 5560  g 5561  l 5562  y 5563  p 5564  t 5565  i 5566  t 5567   
               
               
                 v 5568  p 5569  e 5570  e 5571  f 5572  a 5573  s 5574  h 5575  v 5576  a 5577  n 5578  f 5579  q 5580  k 5581  s 5582  g 5583  y 5584  s 5585  k 5586   
               
               
                 y 5587  v 5588  t 5589  v 5590  q 5591  g 5592  p 5593  p 5594  g 5595  t 5596  g 5597  k 5598  s 5599  h 5600  f 5601  a 5602  i 5603  g 5604  l 5605   
               
               
                 a 5606  i 5607  y 5608  y 5609  p 5610  t 5611  a 5612  r 5613  v 5614  v 5615  y 5616  t 5617  a 5618  c 5619  s 5620  h 5621  a 5622  a 5623  v 5624   
               
               
                 d 5625  a 5626  l 5627  c 5628  e 5629  k 5630  a 5631  f 5632  k 5633  y 5634  l 5635  n 5636  i 5637  a 5638  k 5639  c 5640  s 5641  r 5642  i 5643   
               
               
                 i 5644  p 5645  a 5646  k 5647  a 5648  r 5649  v 5650  e 5651  c 5652  y 5653  d 5654  r 5655  f 5656  k 5657  v 5658  n 5659  e 5660  t 5661  n 5662   
               
               
                 s 5663  q 5664  y 5665  l 5666  f 5667  s 5668  t 5669  i 5670  n 5671  a 5672  l 5673  p 5674  e 5675  t 5676  s 5677  a 5678  d 5679  i 5680  l 5681   
               
               
                 v 5682  v 5683  d 5684  e 5685  v 5686  s 5687  m 5688  c 5689  t 5690  n 5691  y 5692  d 5693  l 5694  s 5695  i 5696  i 5697  n 5698  a 5699  r 5700   
               
               
                 i 5701  k 5702  a 5703  k 5704  h 5705  i 5706  v 5707  y 5708  v 5709  g 5710  d 5711  p 5712  a 5713  q 5714  l 5715  p 5716  a 5717  p 5718  r 5719   
               
               
                 t 5720  l 5721  l 5722  t 5723  r 5724  g 5725  t 5726  l 5727  e 5728  p 5729  e 5730  n 5731  f 5732  n 5733  s 5734  v 5735  t 5736  r 5737  l 5738   
               
               
                 m 5739  c 5740  n 5741  l 5742  g 5743  p 5744  d 5745  i 5746  f 5747  l 5748  s 5749  m 5750  c 5751  y 5752  r 5753  c 5754  p 5755  k 5756  e 5757   
               
               
                 i 5758  v 5759  s 5760  t 5761  v 5762  s 5763  a 5764  l 5765  v 5766  y 5767  n 5768  n 5769  k 5770  l 5771  l 5772  a 5773  k 5774  k 5775  e 5776   
               
               
                 l 5777  s 5778  g 5779  q 5780  c 5781  f 5782  k 5783  i 5784  l 5785  y 5786  k 5787  g 5788  n 5789  v 5790  t 5791  h 5792  d 5793  a 5794  s 5795   
               
               
                 s 5796  a 5797  i 5798  n 5799  r 5800  p 5801  q 5802  l 5803  t 5804  f 5805  v 5806  k 5807  n 5808  f 5809  i 5810  t 5811  a 5812  n 5813  p 5814   
               
               
                 a 5815  w 5816  s 5817  k 5818  a 5819  v 5820  f 5821  i 5822  s 5823  p 5824  y 5825  n 5826  s 5827  q 5828  n 5829  a 5830  v 5831  a 5832  r 5833   
               
               
                 s 5834  m 5835  l 5836  g 5837  l 5838  t 5839  t 5840  q 5841  t 5842  v 5843  d 5844  s 5845  s 5846  q 5847  g 5848  s 5849  e 5850  y 5851  q 5852   
               
               
                 y 5853  v 5854  i 5855  f 5856  c 5857  q 5858  t 5859  a 5860  d 5861  t 5862  a 5863  h 5864  a 5865  n 5866  n 5867  i 5868  n 5869  r 5870  f 5871   
               
               
                 n 5872  v 5873  a 5874  i 5875  t 5876  r 5877  a 5878  q 5879  k 5880  g 5881  i 5882  l 5883  e 5884  v 5885  m 5886  t 5887  s 5888  q 5889  a 5890   
               
               
                 l 5891  f 5892  e 5893  s 5894  l 5895  e 5896  f 5897  t 5898  e 5899  l 5900  s 5901  f 5902  t 5903  n 5904  y 5905  k 5906  l 5907  q 5908  s 5909   
               
               
                 q 5910  i 5911  v 5912  t 5913  g 5914  l 5915  f 5916  k 5917  d 5918  c 5919  s 5920  r 5921  e 5922  t 5923  s 5924  g 5925  l 5926  s 5927  p 5928   
               
               
                 a 5929  y 5930  a 5931  p 5932  t 5933  y 5934  v 5935  s 5936  v 5937  d 5938  d 5939  k 5940  y 5941  k 5942  t 5943  s 5944  d 5945  e 5946  l 5947   
               
               
                 c 5948  v 5949  n 5950  l 5951  n 5952  l 5953  p 5954  a 5955  n 5956  v 5957  p 5958  y 5959  s 5960  r 5961  v 5962  i 5963  s 5964  r 5965  m 5966   
               
               
                 g 5967  f 5968  k 5969  l 5970  d 5971  a 5972  t 5973  v 5974  p 5975  g 5976  y 5977  p 5978  k 5979  l 5980  f 5981  i 5982  t 5983  r 5984  e 5985   
               
               
                 e 5986  a 5987  v 5988  r 5989  q 5990  v 5991  r 5992  s 5993  w 5994  i 5995  g 5996  f 5997  d 5998  v 5999  e 6000  g 6001  a 6002  h 6003  a 6004   
               
               
                 s 6005  r 6006  n 6007  a 6008  c 6009  g 6010  t 6011  n 6012  v 6013  p 6014  l 6015  q 6016  l 6017  g 6018  f 6019  s 6020  t 6021  g 6022  v 6023   
               
               
                 n 6024  f 6025  v 6026  v 6027  q 6028  p 6029  v 6030  g 6031  v 6032  v 6033  d 6034  t 6035  e 6036  w 6037  g 6038  n 6039  m 6040  l 6041  t 6042   
               
               
                 g 6043  i 6044  a 6045  a 6046  r 6047  p 6048  p 6049  p 6050  g 6051  e 6052  q 6053  f 6054  k 6055  h 6056  l 6057  v 6058  p 6059  l 6060  m 6061   
               
               
                 h 6062  k 6063  g 6064  a 6065  a 6066  w 6067  p 6068  i 6069  v 6070  r 6071  r 6072  r 6073  i 6074  v 6075  q 6076  m 6077  l 6078  s 6079  d 6080   
               
               
                 t 6081  l 6082  d 6083  k 6084  l 6085  s 6086  d 6087  y 6088  c 6089  t 6090  f 6091  v 6092  c 6093  w 6094  a 6095  h 6096  g 6097  f 6098  e 6099   
               
               
                 l 6100  t 6101  s 6102  a 6103  s 6104  y 6105  f 6106  c 6107  k 6108  i 6109  g 6110  k 6111  e 6112  q 6113  k 6114  c 6115  c 6116  m 6117  c 6118   
               
               
                 n 6119  r 6120  r 6121  a 6122  a 6123  a 6124  y 6125  s 6126  s 6127  p 6128  l 6129  q 6130  s 6131  y 6132  a 6133  c 6134  w 6135  t 6136  h 6137   
               
               
                 s 6138  c 6139  g 6140  y 6141  d 6142  y 6143  v 6144  y 6145  n 6146  p 6147  f 6148  f 6149  v 6150  d 6151  v 6152  q 6153  q 6154  w 6155  g 6156   
               
               
                 y 6157  v 6158  g 6159  n 6160  l 6161  a 6162  t 6163  n 6164  h 6165  d 6166  r 6167  y 6168  c 6169  s 6170  v 6171  h 6172  q 6173  g 6174  a 6175   
               
               
                 h 6176  v 6177  a 6178  s 6179  n 6180  d 6181  a 6182  i 6183  m 6184  t 6185  r 6186  c 6187  l 6188  a 6189  i 6190  h 6191  s 6192  c 6193  f 6194   
               
               
                 i 6195  e 6196  r 6197  v 6198  d 6199  w 6200  d 6201  i 6202  e 6203  y 6204  p 6205  y 6206  i 6207  s 6208  h 6209  e 6210  k 6211  k 6212  l 6213   
               
               
                 n 6214  s 6215  c 6216  c 6217  r 6218  i 6219  v 6220  e 6221  r 6222  n 6223  v 6224  v 6225  r 6226  a 6227  a 6228  l 6229  l 6230  a 6231  g 6232   
               
               
                 s 6233  f 6234  d 6235  k 6236  v 6237  y 6238  d 6239  i 6240  g 6241  n 6242  p 6243  k 6244  g 6245  i 6246  p 6247  i 6248  v 6249  d 6250  d 6251   
               
               
                 p 6252  v 6253  v 6254  d 6255  w 6256  h 6257  y 6258  f 6259  d 6260  a 6261  q 6262  p 6263  l 6264  t 6265  r 6266  k 6267  v 6268  q 6269  q 6270   
               
               
                 l 6271  f 6272  y 6273  t 6274  e 6275  d 6276  m 6277  a 6278  s 6279  r 6280  f 6281  a 6282  d 6283  g 6284  l 6285  c 6286  l 6287  f 6288  w 6289   
               
               
                 n 6290  c 6291  n 6292  v 6293  p 6294  k 6295  y 6296  p 6297  n 6298  n 6299  a 6300  i 6301  v 6302  c 6303  r 6304  f 6305  d 6306  t 6307  r 6308   
               
               
                 v 6309  h 6310  s 6311  e 6312  f 6313  n 6314  l 6315  p 6316  g 6317  c 6318  d 6319  g 6320  g 6321  s 6322  l 6323  y 6324  v 6325  n 6326  k 6327   
               
               
                 h 6328  a 6329  f 6330  h 6331  t 6332  p 6333  a 6334  y 6335  d 6336  v 6337  s 6338  a 6339  f 6340  r 6341  d 6342  l 6343  k 6344  p 6345  l 6346   
               
               
                 p 6347  f 6348  f 6349  y 6350  y 6351  s 6352  t 6353  t 6354  p 6355  c 6356  e 6357  v 6358  h 6359  g 6360  n 6361  g 6362  s 6363  m 6364  i 6365   
               
               
                 e 6366  d 6367  i 6368  d 6369  y 6370  v 6371  p 6372  l 6373  k 6374  s 6375  a 6376  v 6377  c 6378  i 6379  t 6380  a 6381  c 6382  n 6383  l 6384   
               
               
                 g 6385  g 6386  a 6387  v 6388  c 6389  r 6390  k 6391  h 6392  a 6393  t 6394  e 6395  y 6396  r 6397  e 6398  y 6399  m 6400  e 6401  a 6402  y 6403   
               
               
                 n 6404  l 6405  v 6406  s 6407  a 6408  s 6409  g 6410  f 6411  r 6412  l 6413  w 6414  c 6415  y 6416  k 6417  t 6418  f 6419  d 6420  i 6421  y 6422   
               
               
                 n 6423  l 6424  w 6425  s 6426  t 6427  f 6428  t 6429  k 6430  v 6431  q 6432  g 6433  l 6434  e 6435  n 6436  i 6437  a 6438  f 6439  n 6440  v 6441   
               
               
                 v 6442  k 6443  q 6444  g 6445  h 6446  f 6447  i 6448  g 6449  v 6450  e 6451  g 6452  e 6453  l 6454  p 6455  v 6456  a 6457  v 6458  v 6459  n 6460   
               
               
                 d 6461  k 6462  i 6463  f 6464  t 6465  k 6466  s 6467  g 6468  v 6469  n 6470  d 6471  i 6472  c 6473  m 6474  f 6475  e 6476  n 6477  k 6478  t 6479   
               
               
                 t 6480  l 6481  p 6482  t 6483  n 6484  i 6485  a 6486  f 6487  e 6488  l 6489  y 6490  a 6491  k 6492  r 6493  a 6494  v 6495  r 6496  s 6497  h 6498   
               
               
                 p 6499  d 6500  f 6501  k 6502  l 6503  l 6504  h 6505  n 6506  l 6507  q 6508  a 6509  d 6510  i 6511  c 6512  y 6513  k 6514  f 6515  v 6516  l 6517   
               
               
                 w 6518  d 6519  y 6520  e 6521  r 6522  s 6523  n 6524  i 6525  y 6526  g 6527  t 6528  a 6529  t 6530  i 6531  g 6532  v 6533  c 6534  k 6535  y 6536   
               
               
                 t 6537  d 6538  i 6539  d 6540  v 6541  n 6542  s 6543  a 6544  l 6545  n 6546  i 6547  c 6548  f 6549  d 6550  i 6551  r 6552  d 6553  n 6554  g 6555   
               
               
                 s 6556  l 6557  e 6558  k 6559  f 6560  m 6561  s 6562  t 6563  p 6564  n 6565  a 6566  i 6567  f 6568  i 6569  s 6570  d 6571  r 6572  k 6573  i 6574   
               
               
                 k 6575  k 6576  y 6577  p 6578  c 6579  m 6580  v 6581  g 6582  p 6583  d 6584  y 6585  a 6586  y 6587  f 6588  n 6589  g 6590  a 6591  i 6592  i 6593   
               
               
                 r 6594  d 6595  s 6596  d 6597  v 6598  v 6599  k 6600  q 6601  p 6602  v 6603  k 6604  f 6605  y 6606  l 6607  y 6608  k 6609  k 6610  v 6611  n 6612   
               
               
                 n 6613  e 6614  f 6615  i 6616  d 6617  p 6618  t 6619  e 6620  c 6621  i 6622  y 6623  t 6624  q 6625  s 6626  r 6627  s 6628  c 6629  s 6630  d 6631   
               
               
                 f 6632  l 6633  p 6634  l 6635  s 6636  d 6637  m 6638  e 6639  k 6640  d 6641  f 6642  l 6643  s 6644  f 6645  d 6646  s 6647  d 6648  v 6649  f 6650   
               
               
                 i 6651  k 6652  k 6653  y 6654  g 6655  l 6656  e 6657  n 6658  y 6659  a 6660  f 6661  e 6662  h 6663  v 6664  v 6665  y 6666  g 6667  d 6668  f 6669   
               
               
                 s 6670  h 6671  t 6672  t 6673  l 6674  g 6675  g 6676  l 6677  h 6678  l 6679  l 6680  i 6681  g 6682  l 6683  y 6684  k 6685  k 6686  q 6687  q 6688   
               
               
                 e 6689  g 6690  h 6691  i 6692  i 6693  m 6694  e 6695  e 6696  m 6697  l 6698  k 6699  g 6700  s 6701  s 6702  t 6703  i 6704  h 6705  n 6706  y 6707   
               
               
                 f 6708  i 6709  t 6710  e 6711  t 6712  n 6713  t 6714  a 6715  a 6716  f 6717  k 6718  a 6719  e 6720  c 6721  s 6722  v 6723  i 6724  d 6725  l 6726   
               
               
                 k 6727  l 6728  d 6729  d 6730  f 6731  v 6732  m 6733  i 6734  l 6735  k 6736  s 6737  q 6738  d 6739  l 6740  g 6741  v 6742  v 6743  s 6744  k 6745   
               
               
                 v 6746  v 6747  k 6748  v 6749  p 6750  i 6751  d 6752  l 6753  t 6754  m 6755  i 6756  e 6757  f 6758  m 6759  l 6760  w 6761  c 6762  k 6763  d 6764   
               
               
                 g 6765  q 6766  v 6767  q 6768  t 6769  f 6770  y 6771  p 6772  r 6773  l 6774  q 6775  a 6776  s 6777  a 6778  d 6779  w 6780  k 6781  p 6782  g 6783   
               
               
                 h 6784  a 6785  m 6786  p 6787  s 6788  l 6789  f 6790  k 6791  v 6792  q 6793  n 6794  v 6795  n 6796  l 6797  e 6798  r 6799  c 6800  e 6801  l 6802   
               
               
                 a 6803  n 6804  y 6805  k 6806  q 6807  s 6808  i 6809  p 6810  m 6811  p 6812  r 6813  g 6814  v 6815  h 6816  m 6817  n 6818  i 6819  a 6820  k 6821   
               
               
                 y 6822  m 6823  q 6824  l 6825  c 6826  q 6827  y 6828  l 6829  n 6830  t 6831  c 6832  t 6833  l 6834  a 6835  v 6836  p 6837  a 6838  n 6839  m 6840   
               
               
                 r 6841  v 6842  i 6843  h 6844  f 6845  g 6846  a 6847  g 6848  s 6849  d 6850  k 6851  g 6852  i 6853  a 6854  p 6855  g 6856  t 6857  s 6858  v 6859   
               
               
                 l 6860  r 6861  q 6862  w 6863  l 6864  p 6865  t 6866  d 6867  a 6868  i 6869  i 6870  i 6871  d 6872  n 6873  d 6874  l 6875  n 6876  e 6877  f 6878   
               
               
                 v 6879  s 6880  d 6881  a 6882  d 6883  i 6884  t 6885  l 6886  f 6887  g 6888  d 6889  c 6890  v 6891  t 6892  v 6893  r 6894  v 6895  g 6896  q 6897   
               
               
                 q 6898  v 6899   d 6900  l 6901  v 6902  i 6903  s 6904  d 6905  m 6906  y 6907  d 6908  p 6909  t 6910  t 6911  k 6912  n 6913  v 6914  t 6915  g 6916   
               
               
                 s 6917  n 6918  e 6919  s 6920  k 6921  a 6922  l 6923  f 6924  f 6925  t 6926  y 6927  l 6928  c 6929  n 6930  l 6931  i 6932  n 6933  n 6934  n 6935   
               
               
                 l 6936  a 6937  l 6938  g 6939  g 6940  s 6941  v 6942  a 6943  i 6944  k 6945  i 6946  t 6947  e 6948  h 6949  s 6950  w 6951  s 6952  v 6953  e 6954   
               
               
                 l 6955 y 6956  e 6957  l 6958  m 6959  g 6960  k 6961  f 6962  a 6963  w 6964  w 6965  t 6966  v 6967  f 6968  c 6969  t 6970  n 6971  a 6972  n 6973   
               
               
                 a 6974  s 6975  s 6976  s 6977  e 6978  g 6979  f 6980  l 6981  l 6982  g 6983  i 6984  n 6985  y 6986  l 6987  g 6988  t 6989  i 6990  k 6991  e 6992   
               
               
                 n 6993  i 6994  d 6995  g 6996  g 6997  a 6998  m 6999  h 7000  a 7001  n 7002  y 7003  i 7004  f 7005  w 7006  r 7007  n 7008  s 7009  t 7010  p 7011   
               
               
                 m 7012  n 7013  l 7014  s 7015  t 7016  y 7017  s 7018  l 7019  f 7020  d 7021  l 7022  s 7023  k 7024  f 7025  q 7026  l 7027  k 7028  l 7029  k 7030   
               
               
                 g 7031  t 7032  p 7033  v 7034  l 7035  q 7036  l 7037  k 7038  e 7039  s 7040  q 7041  i 7042  n 7043  e 7044  l 7045  v 7046  i 7047  s 7048  l 7049   
               
               
                 l 7050  s 7051  q 7052  g 7053  k 7054  l 7055  l 7056  i 7057  r 7058  d 7059  n 7060  d 7061  t 7062  l 7063  s 7064  v 7065  s 7066  t 7067  d 7068   
               
               
                 v 7069  l 7070  v 7071  n 7072  t 7073  y 7074  r 7075  k 7076  l 7077  r 7078   
               
               
                   
               
               
                 Amino-terminal 
               
               
                 (SEQ ID NO: 30) 
                   
               
               
                 h 25 _q 26 _d 27 _h 28 _v 29 _s 30 _l 31 _t 32 _v 33 _p 34 _l 35 _c 36 _g 37 _s 38 _g 39 _n 40 _l 41 _v 42 _e 43 _k 44 _l 45 _s 46 _p 47 _w 48 _f 49 _m 50 _ 
                   
               
               
                 d 51 _g 52  e 53 _n 54 _a 55 _y 56 _e 57 _v 58 _v 59 _k 60 _a 61 _m 62 _l 63 _l 64 _k 65 _k 66   
               
               
                   
               
               
                 (SEQ ID NO: 31) 
                   
               
               
                 h 28 _v 29 _s 30 _l 31 _t 32 _v 33 _p 34 _l 35 _c 36 _g 37 _s 38 _g 39 _n 40 _l 41 _v 42 _e 43 _k 44 _l 45 _s 46 _p 47 _w 48 _f 49 _m 50 _d 51 _g 52 _e 53 _ 
                   
               
               
                 n 54 _a 55  y 56 _e 57 _v 58 _v 59 _k 60 _a 61 _m 62 _l 63 _l 64 _l 65 _k 66   
               
               
                   
               
               
                 (SEQ ID NO: 15) 
                   
               
               
                 k 60 _a 61 _m 62 _l 63 _l 64 _k 65 _k 66 _e 67 _p 68 _l 69 _l 70 _y 71 _v 72 _p 73 _i 74 _r 75 _l 76 _a 77 _g 78 _h 79   
                   
               
               
                   
               
               
                 (SEQ ID NO: 32) 
                   
               
               
                 k 60 _a 61 _m 62 _l 63 _l 64 _k 65 _k 66 _e 67 _p 68 _l 69 _l 70 _y 71 _v 72 _p 73 _i 74 _r 75 _l 76 _a 77 _g 78 _h 79 _t 80 _r 81 _h 82   
                   
               
               
                   
               
               
                 (SEQ ID NO: 33) 
                   
               
               
                 k 140 _q 141 _n 142 _i 143 _l 144 _l 145 _r 146 _k 147 _y 148 _g 149 _r 150 _g 151 _g 152 _y 153 _h 154   
                   
               
               
                   
               
               
                 (SEQ ID NO: 34) 
                   
               
               
                 k 140 _q 141 _n 142 _i 143 _l 144 _l 145 _r 146 _k 147 _y 148 _g 149 _r 150 _g 151 _g 152 _y153_h 154 _y 155 _t 156 _p 157 _f 158 _h 159   
                   
               
               
                   
               
               
                 (SEQ ID NO: 35) 
                   
               
               
                 h 154 _y 155 _t 156 _p 157 _f 158 _h 159 _y 160 _e 161 _r 162 _d 163 _n 164 _t 165 _s 166 _c 167 _p 168 _e 169 _w 170 _m 171 _d 172 _d 173 _f 174 _e 175 _ 
                   
               
               
                 a 176  d 177 _p 178 _k 179 _g 180 _k 181 _y 182 _a 183 _q 184 _n 185 _l 186 _l 187 _k 188   
               
               
                   
               
               
                 (SEQ ID NO: 36) 
                   
               
               
                 h 154 _y 155 _t 156 _p 157 _f 158 _h 159 _y 160 _e 161 _r 162 _d 163 _n 164 _t 165 _s 166 _c 167 _p 168 _e 169 _w 170 _m 171 _d 172 _d 173 _f 174 _e 175 _ 
                   
               
               
                 a 176  d 177 _p 178 _k 179 _g 180 _k 181 _y 182 _a 183 _q 184 _n 185 _l 186 _l 187 _k 188 _k 189   
               
               
                   
               
               
                 (SEQ ID NO: 37) 
                   
               
               
                 h 159 _y 160 _e 161 _r 162 _d 163 _n 164 _t 165 _s 166 _c 167 _p 168 _e 169 _w 170 _m 171 _d 172 _d 173 _f 174 _e 175 _a 176 _d 177 _p 178 _k 179 _g 180 _ 
                   
               
               
                 k 181  y 182 _a 183 _q 184 _n 185 _l 186 _l 187 _k 188   
               
               
                   
               
               
                 (SEQ ID NO: 38) 
                   
               
               
                 h 159 _y 160 _e 161 _r 162 _d 163 _n 164 _t 165 _s 166 _c 167 _p 168 _e 169 _w 170 _m 171 _d 172 _d 173 _f 174 _e 175 _a 176 _d 177 _p 178 _k 179 _g 180 _ 
                   
               
               
                 k 181  y 182 _a 183 _q 184 _n 185 _l 186 _l 187 _k 188 _k 189   
               
               
                   
               
               
                 (SEQ ID NO: 39) 
                   
               
               
                 h 254 _v 255 _e 256 _r 257 _k 258 _d 259 _v 260 _p 261 _y 262 _p 263 _k 264 _q 265 _s 266 _i 267 _f 268 _t 269 _i 270 _n 271 _s 272 _v 273 _v 274 _q 275 _ 
                   
               
               
                 k 276  d 277 _g 278 _v 279 _e 280 _n 281 _t 282 _p 283 _p 284 _h 285 _y 286 _f 287 _t 288 _l 289 _g 290 _c 291 _k 292 _i 293 _l 294 _t 295 _l 296 _t 297 _ 
               
               
                 p 298 _r 299 _n 300  k 301   
               
               
                   
               
               
                 (SEQ ID NO: 26) 
                   
               
               
                 h 254 _v 255 _e 256 _r 257 _k 258 _d 259 _v 260 _p 261 _y 262 _p 263 _k 264   
                   
               
               
                   
               
               
                 (SEQ ID NO: 40) 
                   
               
               
                 k 258 _d 259 _v 260 _p 261 _y 262 _p 263 _k 264 _q 265 _s 266 _i 267 _f 268 _t 269 _i 270 _n 271 _s 272 _v 273 _v 274 _q 275 _k 276 _d 277 _g 278 _v 279 _ 
                   
               
               
                 e 280  n 281 _t 282 _p 283 _p 284 _h 285   
               
               
                   
               
               
                 (SEQ ID NO: 41) 
                   
               
               
                 h 285 _y 286 _f 287 _t 288 _l 289 _g 290 _c 291 _k 292 _i 293 _l 294 _t 295 _l 296 _t 297 _p 298 _r 299 _n 300 _k 301   
                   
               
               
                   
               
               
                 (SEQ ID NO: 42) 
                   
               
               
                 h 285 _y 286 _f 287 _t 288 _l 289 _g 290 _c 291 _k 292 _i 293 _l 294 _t 295 _l 296 _t 297 _p 298 _r 299 _n 300 _k 301 _w 302 _s 303 _g 304 _v 305 _s 306 _ 
                   
               
               
                 d 307 _l 308  s 309 _l 310 _k 311   
               
               
                   
               
               
                 (SEQ ID NO: 43) 
                   
               
               
                 h 285 _y 286 _f 287 _t 288 _l 289 _g 290 _c 291 _k 292 _i 293 _l 294 _t 295 _l 296 _t 297 _p 298 _r 299 _n 300 _k 301 _w 302 _s 303 _g 304 _v 305 _s 306 _ 
                   
               
               
                 d 307 _l 308  s 309 _l 310 _k 311 _q 312 _k 313 _l 314 _l 315 _y 316 _t 317 _f 318 _y 319 _g 320 _k 321   
               
               
                   
               
               
                 (SEQ ID NO: 44) 
                   
               
               
                 k 292 _i 293 _l 294 _t 295 _l 296 _t 297 _p 298 _r 299 _n 300 _k 301 _w 302 _s 303 _g 304 _v 305 _s 306 _d 307 _l 308 _s 309 _l 310 _k 311 _q 312 _k 313 _ 
                   
               
               
                 l 314 _l 315  y 316 _t 317 _f 318 _y 319 _g 320 _k 321 _e 322 _s 323 _l 324 _e 325 _n 326 _p 327 _t 328 _y 329 _i 330 _y 331 _h 332   
               
               
                   
               
               
                 (SEQ ID NO: 45) 
                   
               
               
                 k 301 _w 302 _s 303 _g 304 _v 305 _s 306 _d 307 _l 308 _s 309 _l 310 _k 311 _q 312 _k 313 _l 314 _l 315 _y 316 _t 317 _f 318 _y 319 _g 320 _k 321 _e 322 _ 
                   
               
               
                 s 323 _l 324  e 325 _n 326 _p 327 _t 328 _y 329 _i 330 _y 331 _h 332   
               
               
                   
               
               
                 (SEQ ID NO: 46) 
                   
               
               
                 k 311 _q 312 _k 313 _l 314 _l 315 _y 316 _t 317 _f 318 _y 319 _g 320 _k 321 _e 322 _s 323 _l 324 _e 325 _n 326 _p 327 _t 328 _y 329 _i 330 _y 331 _h 332   
                   
               
               
                   
               
               
                 (SEQ ID NO: 47) 
                   
               
               
                 k 313 _l 314 _l 315 _y 316 _t 317 _f 318 _y 319 _g 320 _k 321 _e 322 _s 323 _l 324 _e 325 _n 326 _p 327 _t 328 _y 329 _i 330 _y 331 _h 332   
                   
               
               
                   
               
               
                 (SEQ ID NO: 13) 
                   
               
               
                 k 390 _g 391 _d 392 _s 393 _c 394 _s 395 _s 396 _n 397 _c 398 _k 399 _h 400   
                   
               
               
                   
               
               
                 (SEQ ID NO: 48) 
                   
               
               
                 h 482 _s 483 _l 484 _n 485 _f 486 _v 487 _g 488 _e 489 _f 490 _v 491 _v 492 _n 493 _d 494 _v 495 _v 496 _l 497 _a 498 _i 499 _l 500 _s 501 _g 502 _t 503 _ 
                   
               
               
                 t 504 _t 505  n 506 _v 507 _d 508 _k 509 _i 510 _r 511 _q 512 _l 513 _l 514 _k 515 _g 516 _v 517 _t 518 _l 519 _d 520 _k 521   
               
               
                   
               
               
                 (SEQ ID NO: 49) 
                   
               
               
                 k 570 _k 571 _v 572 _a 573 _t 574 _i 575 _p 576 _y 577 _k 578 _v 579 _c 580 _n 581 _s 582 _v 583 _k 584 _d 585 _t 586 _l 587 _t 588 _y 589 _y 590 _a 591 _ 
                   
               
               
                 h 592   
               
               
                   
               
               
                 (SEQ ID NO: 50) 
                   
               
               
                 k 571 _v 572 _a 573 _t 574 _i 575 _p 576 _y 577 _k 578 _v 579 _c 580 _n 581 _s 582 _v 583 _k 584 _d 585 _t 586 _l 587 _t 588 _y 589 _y 590 _a 591 _h 592   
                   
               
               
                   
               
               
                 (SEQ ID NO: 51) 
                   
               
               
                 k 578 _v 579 9_c 580 _n 581 _s 582 _v 583 _k 584 _d 585 _t 586 _l 587 _t 588 _y 589 _y 590 _a 591 _h 592   
                   
               
               
                   
               
               
                 (SEQ ID NO: 52) 
                   
               
               
                 k 697 _v 698 _s 699 _t 700 _l 701 _v 702 _k 703 _q 704 _v 705 _l 706 _d 707 _l 708 _l 709 _n 710 _k 711 _g 712 _m 713 _q 714 _l 715 _l 716 _h 717   
                   
               
               
                   
               
               
                 (SEQ ID NO: 53) 
                   
               
               
                 k 703 _q 704 _v 705 _l 706 _d 707 _l 708 _l 709 _n 710 _k 711 _g 712 _m 713 _q 714 _l 715 _l 716 _h 717   
                   
               
               
                   
               
               
                 (SEQ ID NO: 27) 
                   
               
               
                 k 711 _g 712 _m 713 _q 714 _l 715 _l 716 _h 717 _t 718 _k 719   
                   
               
               
                   
               
               
                 (SEQ ID NO: 54) 
                   
               
               
                 h 806 _s 807 _p 808 _d 809 _v 810 _i 811 _v 812 _g 813 _d 814 _y 815 _v 816 _i 817 _i 818 _s 819 _e 820 _k 821 _l 822 _f 823 _v 824 _r 825 _s 826 _k 827   
                   
               
               
                   
               
               
                 (SEQ ID NO: 55) 
                   
               
               
                 k 821 _l 822 _f 823 _v 824 _r 825 _s 826 _k 827 _e 828 _e 829 _d 830 _g 831 _f 832 _a 833 _f 834 _y 835 _p 836 _a 837 _c 838 _t 839 _n 840 _g 841 _h 842   
                   
               
               
                   
               
               
                 (SEQ ID NO: 56) 
                   
               
               
                 k 821 _l 822 _f 823 _v 824 _r 825 _s 826 _k 827 _e 828 _e 829 _d 830 _g 831 _f 832 _a 833 _f 834 _y 835 _p 836 _a 837 _c 838 _t 839 _n 840 _g 841 _h 842 _ 
                   
               
               
                 a 843  v 844 _p 845 _t 846 _l 847 _f 848 _r 849 _l 850 _k 851 _g 852 _g 853 _a 854 _p 855 _v 856 _k 857 _k 858 _v 859 _a 860 _f 861 _g 862 _g 863 _d 864 _ 
               
               
                 q 865 _v 866  h 867   
               
               
                   
               
               
                 (SEQ ID NO: 57) 
                   
               
               
                 h 842 _a 843 _v 844 _p 845 _t 846 _l 847 _f 848 _r 849 _l 850 _k 851 _g 852 _g 853 _a 854 _p 855 _v 856 _k 857   
                   
               
               
                   
               
               
                 (SEQ ID NO: 58) 
                   
               
               
                 h 842 _a 843 _v 844 _p 845 _t 846 _l 847 _f 848 _r 849 _l 850 _k 851 _g 852 _g 853 _a 854 _p 855 _v 856 _k 857 _k 858   
                   
               
               
                   
               
               
                 (SEQ ID NO: 59) 
                   
               
               
                 k 851 _g 852 _g 853 _a 854 _p 855 _v 856 _k 857 _k 858 _v 859 _a 860 _f 861 _g 862 _g 863 _d 864 _q 865 _v 866 _h 867   
                   
               
               
                   
               
               
                 (SEQ ID NO: 60) 
                   
               
               
                 k 851 _g 852 _g 853 _a 854 _p 855 _v 856 _k 857 _k 858 _v 859 _a 860 _f 861 _g 862 _g 863 _d 864 _q 865 _v 866 _h 867 _e 868 _v 869 _a 870 _a 871 _v 872 _ 
                   
               
               
                 r 873  s 874 _v 875 _t 876 _v 877 _e 878 _y 879 _n 880 _i 881 _h 882   
               
               
                   
               
               
                 (SEQ ID NO: 61) 
                   
               
               
                 h 882 _a 883 _v 884 _l 885 _d 886 _t 887 _l 888 _l 889 _a 890 _s 891 _s 892 _s 893 _l 894 _r 895 _t 896 _f 897 _v 898 _v 899 _d 900 _k 901 _s 902 _l 903 _ 
                   
               
               
                 s 904 _i 905  e 906 _e 907 _f 908 _a 909 _d 910 _v 911 _v 912 _k 913 _e 914 _q 915 _v 916 _s 917 _d 918 _l 919 _l 920 _v 921 _k 922   
               
               
                   
               
               
                 (SEQ ID NO: 62) 
                   
               
               
                 k 1098 _p 1099 _k 1100 _r 1101 _l 1102 _r 1103 _k 1104 _k 1105 _r 1106 _n 1107 _v 1108 _d 1109 _p 1110 _l 1111 _s 1112 _n 1113 _f 1114 _e 1115 _h 1116   
                   
               
               
                   
               
               
                 (SEQ ID NO: 63) 
                   
               
               
                 h 1149 _l 1150 _k 1151 _h 1152 _g 1153 _g 1154 _g 1155 _i 1156 _a 1157 _g 1158 _a 1159 _i 1160 _n 1161 _a 1162 _a 1163 _s 1164 _k 1165 _g 1166 _a 1167   
                   
               
               
                 v 1168 _q 1169 _k 1170 _e 1171 _s 1172 _d 1173 _e 1174 _y 1175 _i 1176 _l 1177 _a 1178 _k 1179   
               
               
                   
               
               
                 (SEQ ID NO: 64) 
                   
               
               
                 h 1152 _g 1153 _g 1154 _g 1155 _i 1156 _a 1157 _g 1158 _a 1159 _i 1160 _n 1161 _a 1162 _a 1163 _s 1164 _k 1165 _g 1166 _a 1167 _v 1168 _q 1169 _k 1170   
                   
               
               
                 e 1171 _s 1172 _d 1173 _e 1174 _y 1175 _i 1176 _l 1177 _a 1178 _k 1179   
               
               
                   
               
               
                 (SEQ ID NO: 65) 
                   
               
               
                 k 1170 _e 1171 _s 1172 _d 1173 _e 1174 _y 1175 _i 1176 _l 1177 _a 1178 _k 1179 _g 1180 _p 1181 _l 1182 _q 1183 _v 1184 _g 1185 _d 1186 _s 1187 _v 1188 _ 
                   
               
               
                 l 1189  l 1190 _q 1191 _g 1192 _h 1193 _s 1194 _l 1195 _a 1196 _k 1197 _n 1198 _i 1199 _l 1200 _h 1201   
               
               
                   
               
               
                 (SEQ ID NO: 66) 
                   
               
               
                 k 1170 _e 1171 _s 1172 _d 1173 _e 1174 _y 1175 _i 1176 _l 1177 _a 1178 _k 1179 _g 1180 _p 1181 _l 1182 _q 1183 _v 1184 _g 1185 _d 1186 _s 1187 _v 1188 _ 
                   
               
               
                 l 1189  l 1190 _q 1191 _g 1192 _h 1193   
               
               
                   
               
               
                 (SEQ ID NO: 67) 
                   
               
               
                 h 1193 _s 1194 _l 1195 _a 1196 _k 1197 _n 1198 _i 1199 _l 1200 _h 1201 _v 1202 _v 1203 _g 1204 _p 1205 _d 1206 _a 1207 _r 1208 _a 1209 _k 1210 _q 1211   
                   
               
               
                 d 1212 _v 1213 _s 1214 _l 1215 _l 1216 _s 1217 _k 1218   
               
               
                   
               
               
                 (SEQ ID NO: 68) 
                   
               
               
                 h 1201 _v 1202 _v 1203 _g 1204 _p 1205 _d 1206 _a 1207 _r 1208 _a 1209 _k 1210 _q 1211 _d 1212 _v 1213 _s 1214 _l 1215 _l 1216 _s 1217 _k 1218   
                   
               
               
                   
               
               
                 (SEQ ID NO: 69) 
                   
               
               
                 k 1317 _g 1318 _v 1319 _d 1320 _y 1321 _t 1322 _k 1323 _k 1324 _f 1325 _l 1326 _t 1327 _v 1328 _d 1329 _g 1330 _v 1331 _q 1332 _y 1333 _y 1334 _c 1335   
                   
               
               
                 y 1336 _t 1337 _s 1338 _k 1339 _d 1340 _t 1341 _l 1342 _d 1343 _d 1344 _i 1345 _l 1346 _q 1347 _q 1348 _a 1349 _n 1350 _k 1351 _s 1352 _v 1353 _g 1354 _ 
               
               
                 i 1355  i 1356 _s 1357 _m 1358 _p 1359 _l 1360 _g 1361 _y 1362 _v 1363 _s 1364 _h 1365   
               
               
                   
               
               
                 (SEQ ID NO: 70) 
                   
               
               
                 h 1365 _g 1366 _l 1367 _d 1368 _l 1369 _i 1370 _q 1371 _a 1372 _g 1373 _s 1374 _v 1375 _v 1376 _r 1377 _r 1378 _v 1379 _n 1380 _v 1381 _p 1382 _y 1383 _ 
                   
               
               
                 v 1384  c 1385 _l 1386 _l 1387 _a 1388 _n 1389 _k 1390 _e 1391 _q 1392 _e 1393 _a 1394 _i 1395 _l 1396 _m 1397 _s 1398 _e 1399 _d 1400 _v 1401 _k 1402 _ 
               
               
                 l 1403 _n 1404  p 1405 _s 1406 _e 1407 _d 1408 _f 1409 _i 1410 _k 1411   
               
               
                   
               
               
                 (SEQ ID NO: 13) 
                   
               
               
                 k 1402 _l 1403 _n 1404 _p 1405 _s 1406 _e 1407 _d 1408 _f 1409 _i 1410 _k 1411 _h 1412   
                   
               
               
                   
               
               
                 (SEQ ID NO: 71) 
                   
               
               
                 k 1402 _l 1403 _n 1404 _p 1405 _s 1406 _e 1407 _d 1408 _f 1409 _i 1410 _k 1411 _h 1412 _v 1413 _r 1414 _t 1415 _n 1416 _g 1417 _g 1418 _y 1419 _n 1420 _ 
                   
               
               
                 s 1421  w 1422 _h 1423   
               
               
                   
               
               
                 (SEQ ID NO: 72) 
                   
               
               
                 k 1402 _l 1403 _n 1404 _p 1405 _s 1406 _e 1407 _d 1408 _f 1409 _i 1410 _k 1411 _h 1412 _v 1413 _r 1414 _t 1415 _n 1416 _g 1417 _g 1418 _y 1419 _n 1420 _ 
                   
               
               
                 s 1421  w 1422 _h 1423 _l 1424 _v 1425 _e 1426 _g 1427 _e 1428 _l 1429 _l 1430 _v 1431 _q 1432 _d 1433 _l 1434 _r 1435 _l 1436 _n 1437 _k 1438 _l 1439 _ 
               
               
                 l 1440 _h 1441   
               
               
                   
               
               
                 (SEQ ID NO: 73) 
                   
               
               
                 h 1412 _v 1413 _r 1414 _t 1415 _n 1416 _g 1417 _g 1418 _y 1419 _n 1420 _s 1421 _w 1422 _h 1423 _l 1424 _v 1425 _e 1426 _g 1427 _e 1428 _l 1429 _l 1430 _ 
                   
               
               
                 v 1431  q 1432 _d 1433 _l 1434 _r 1435 _l 1436 _n 1437 _k 1438 _l 1439 _l 1440 _h 1441 _w 1442 _s 1443 _d 1444 _q 1445 _t 1446 _i 1447 _c 1448 _y 1449 _ 
               
               
                 k 1450 _d 1451  s 1452 _v 1453 _f 1454 _y 1455 _v 1456 _v 1457 _k 1458   
               
               
                   
               
               
                 (SEQ ID NO: 74) 
                   
               
               
                 h 1423 _l 1424 _v 1425 _e 1426 _g 1427 _e 1428 _l 1429 _l 1430 _v 1431 _q 1432 _d 1433 _l 1434 _r 1435 _l 1436 _n 1437 _k 1438 _l 1439 _l 1440 _h 1441 _ 
                   
               
               
                 w 1442  s 1443 _d 1444 _q 1445 _t 1446 _i 1447 _c 1448 _y 1449 _k 1450 _d 1451 _s 1452 _v 1453 _f 1454 _y 1455 _v 1456 _v 1457 _k 1458   
               
               
                   
               
               
                 (SEQ ID NO: 75) 
                   
               
               
                 h 1441 _w 1442 _s 1443 _d 1444 _q 1445 _t 1446 _i 1447 _c 1448 _y 1449 _k 1450 _d 1451 _s 1452 _v 1453 _f 1454 _y 1455 _v 1456 _v 1457 _k 1458   
                   
               
               
                   
               
               
                 (SEQ ID NO: 76) 
                   
               
               
                 h 1533 _s 1534 _l 1535 _y 1536 _l 1537 _a 1538 _d 1539 _n 1540 _l 1541 _t 1542 _a 1543 _d 1544 _e 1545 _t 1546 _k 1547 _a 1548 _l 1549 _k 1550 _e 1551 _ 
                   
               
               
                 l 1552  y 1553 _g 1554 _p 1555 _v 1556 _d 1557 _p 1558 _t 1559 _f 1560 _l 1561 _h 1562 _r 1563 _f 1564 _y 1565 _s 1566 _l 1567 _k 1568 _a 1569 _a 1570 _ 
               
               
                 v 1571 _h 1572  k 1573 _w 1574 _k 1575   
               
               
                   
               
               
                 (SEQ ID NO: 77) 
                   
               
               
                 h 1533 _s 1534 _l 1535 _y 1536 _l 1537 _a 1538 _d 1539 _n 1540 _l 1541 _t 1542 _a 1543 _d 1544 _e 1545 _t 1546 _k 1547 _a 1548 _l 1549 _k 1550 _e 1551 _ 
                   
               
               
                 l 1552  y 1553 _g 1554 _p 1555 _v 1556 _d 1557 _p 1558 _t 1559 _f 1560 _l 1561 _h 1562 _r 1563 _f 1564 _y 1565 _s 1566 _l 1567 _k 1568 _a 1569 _a 1570 _ 
               
               
                 v 1571 _h 1572  k 1573 _w 1574 _k 1575 _m 1576 _v 1577 _v 1578 _c 1579 _d 1580 _k 1581   
               
               
                   
               
               
                 (SEQ ID NO: 78) 
                   
               
               
                 h 1562 _r 1563 _f 1564 _y 1565 _s 1566 _l 1567 _k 1568 _a 1569 _a 1570 _v 1571 _h 1572 _k 1573 _w 1574 _k 1575 _m 1576 _v 1577 _v 1578 _c 1579 _d 1580   
                   
               
               
                 k1581 
               
               
                   
               
               
                 (SEQ ID NO: 79) 
                   
               
               
                 h 1562 _r 1563 _f 1564 _y 1565 _s 1566 _l 1567 _k 1568 _a 1569 _a 1570 _v 1571 _h 1572 _k 1573 _w 1574 _k 1575   
                   
               
               
                   
               
               
                 (SEQ ID NO: 80) 
                   
               
               
                 k 1568 _a 1569 _a 1570 _v 1571 _h 1572 _k 1573 _w 1574 _k 1575 _m 1576 _v 1577 _v 1578 _c 1579 _d 1580 _k 1581 _v 1582 _r 1583 _s 1584 _l 1585 _k 1586   
                   
               
               
                 l 1587 _s 1588 _d 1589 _n 1590 _n 1591 _c 1592 _y 1593 _l 1594 _n 1595 _a 1596 _v 1597 _i 1598 _m 1599 _t 1600 _l 1601 _d 1602 _l 1603 _l 1604 _k 1605 _ 
               
               
                 d 1606  i 1607 _k 1608 _f 1609 _v 1610 _i 1611 _p 1612 _a 1613 _l 1614 _q 1615 _h 1616   
               
               
                   
               
               
                 (SEQ ID NO: 18) 
                   
               
               
                 k 1568 _a 1569 _a 1570 _v 1571 _h 1572 _k 1573 _w 1574 _k 1575   
                   
               
               
                   
               
               
                 (SEQ ID NO: 7) 
                   
               
               
                 h 1572 _k 1573 _w 1574 _k 1575 _m 1576 _v 1577 _v 1578 _c 1579 _d 1580 _k 1581   
                   
               
               
                   
               
               
                 (SEQ ID NO: 81) 
                   
               
               
                 k 1573 _w 1574 _k 1575 _m 1576 _v 1577 _v 1578 _c 1579 _d 1580 _k 1581 _v 1582 _r 1583 _s 1584 _l 1585 _k 1586 _l 1587 _s 1588 _d 1589 _n 1590 _n 1591   
                   
               
               
                 c 1592 _y 1593 _l 1594 _n 1595 _a 1596 _v 1597 _i 1598 _m 1599 _t 1600 _l 1601 _d 1602 _l 1603 _l 1604 _k 1605 _d 1606 _i 1607 _k 1608 _f 1609 _v 1610 _ 
               
               
                 i 1611  p 1612 _a 1613 _l 1614 _q 1615 _h 1616   
               
               
                   
               
               
                 (SEQ ID NO: 82) 
                   
               
               
                 k 1573 _w 1574 _k 1575 _m 1576 _v 1577 _v 1578 _c 1579 _d 1580 _k 1581 _v 1582 _r 1583 _s 1584 _l 1585 _k 1586 _l 1587 _s 1588 _d 1589 _n 1590 _n 1591   
                   
               
               
                 c 1592 _y 1593 _l 1594 _n 1595 _a 1596 _v 1597 _i 1598 _m 1599 _t 1600 _l 1601 _d 1602 _l 1603 _l 1604 _k 1605 _d 1606 _i 1607 _k 1608 _f 1609 _v 1610 _ 
               
               
                 i 1611  p 1612 _a 1613 _l 1614 _q 1615 _h 1616 _a 1617 _f 1618 _m 1619 _k 1620 _h 1621   
               
               
                   
               
               
                 (SEQ ID NO: 83) 
                   
               
               
                 k 1575 _m 1576 _v 1577 _v 1578 _c 1579 _d 1580 _k 1581 _v 1582 _r 1583 _s 1584 _l 1585 _k 1586 _l 1587 _s 1588 _d 1589 _n 1590 _n 1591 _c 1592 _y 1593   
                   
               
               
                 l 1594 _n 1595 _a 1596 _v 1597 _i 1598 _m 1599 _t 1600 _l 1601 _d 1602 _l 1603 _l 1604 _k 1605 _d 1606 _i 1607 _k 1608 _f 1609 _v 1610 _i 1611 _p 1612 _ 
               
               
                 a 1613  l 1614 _q 1615 _h 1616   
               
               
                   
               
               
                 (SEQ ID NO: 84) 
                   
               
               
                 k 1575 _m 1576 _v 1577 _v 1578 _c 1579 _d 1580 _k 1581 _v 1582 _r 1583 _s 1584 _l 1585 _k 1586 _l 1587 _s 1588 _d 1589 _n 1590 _n 1591 _c 1592 _y 1593   
                   
               
               
                 l 1594 _n 1595 _a 1596 _v 1597 _i 1598 _m 1599 _t 1600 _l 1601 _d 1602 _l 1603 _l 1604 _k 1605 _d 1606 _i 1607 _k 1608 _f 1609 _v 1610 _i 1611 _p 1612 _ 
               
               
                 a 1613  l 1614 _q 1615 _h 1616 _a 1617 _f 1618 _m 1619 _k 1620 _h 1621   
               
               
                   
               
               
                 (SEQ ID NO: 85) 
                   
               
               
                 h 1652 _t 1653 _v 1654 _l 1655 _a 1656 _k 1657 _a 1658 _e 1659 _l 1660 _c 1661 _c 1662 _s 1663 _a 1664 _r 1665 _m 1666 _v 1667 _w 1668 _r 1669 _e 1670   
                   
               
               
                 w 1671 _c 1672 _n 1673 _v 1674 _c 1675 _g 1676 _i 1677 _k 1678 _d 1679 _v 1680 _v 1681 _l 1682 _q 1683 _g 1684 _l 1685 _k 1686   
               
               
                   
               
               
                 (SEQ ID NO: 86) 
                   
               
               
                 h 1759 _y 1760 _v 1761 _h 1762 _a 1763 _r 1764 _l 1765 _k 1766 _g 1767 _g 1768 _l 1769 _i 1770 _l 1771 _k 1772 _f 1773 _d 1774 _s 1775 _g 1776 _t 1777 _ 
                   
               
               
                 v 1778  s 1779 _k 1780   
               
               
                   
               
               
                 (SEQ ID NO: 87) 
                   
               
               
                 h 1759 _y 1760 _v 1761 _h 1762 _a 1763 _r 1764 _l 1765 _k 1766 _g 1767 _g 1768 _l 1769 _i 1770 _l 1771 _k 1772 _f 1773 _d 1774 _s 1775 _g 1776 _t 1777 _ 
                   
               
               
                 v 1778  s 1779 _k 1780 _t 1781 _s 1782 _d 1783 _w 1784 _k 1785 _c 1786 _k 1787   
               
               
                   
               
               
                 (SEQ ID NO: 88) 
                   
               
               
                 h 1759 _y 1760 _v 1761 _h 1762 _a 1763 _r 1764 _l 1765 _k 1766 _g 1767 _g 1768 _l 1769 _i 1770 _l 1771 _k 1772 _f 1773 _d 1774 _s 1775 _g 1776 _t 1777 _ 
                   
               
               
                 v 1778  s 1779 _k 1780 _t 1781 _s 1782 _d 1783 _w 1784 _k 1785 _c 1786 _k 1787 _v 1788 _d 1790 _v 1791 _l 1792 _f 1793 _s 1794 _g 1795 _q 1796 _k 1797   
               
               
                   
               
               
                 (SEQ ID NO: 89) 
                   
               
               
                 h 1759 _y 1760 _v 1761 _h 1762 _a 1763 _r 1764 _l 1765 _k 1766 _g 1767 _g 1768 _l 1769 _i 1770 _l 1771 _k 1772   
                   
               
               
                   
               
               
                 (SEQ ID NO: 14) 
                   
               
               
                 h 1762 _a 1763 _r 1764 _l 1765 _k 1766 _g 1767 _g 1768 _l 1769 _i 1770 _l 1771 _k 1772   
                   
               
               
                   
               
               
                 (SEQ ID NO: 90) 
                   
               
               
                 h 1762 _a 1763 _r 1764 _l 1765 _k 1766 _g 1767 _g 1768 _l 1769 _i 1770 _l 1771 _k 1772 _f 1773 _d 1774 _s 1775 _g 1776 _t 1777 _v 1778 _s 1779 _k 1780   
                   
               
               
                   
               
               
                 (SEQ ID NO: 91) 
                   
               
               
                 h 1762 _a 1763 _r 1764 _l 1765 _k 1766 _g 1767 _g 1768 _l 1769 _i 1770 _l 1771 _k 1772 _f 1773 _d 1774 _s 1775 _g 1776 _t 1777 _v 1778 _s 1779 _k 1780 _ 
                   
               
               
                 t 1781  s 1782 _d 1783 _w 1784 _k 1785 _c 1786 _k 1787   
               
               
                   
               
               
                 (SEQ ID NO: 92) 
                   
               
               
                 h 1762 _a 1763 _r 1764 _l 1765 _k 1766 _g 1767 _g 1768 _l 1769 _i 1770 _l 1771 _k 1772 _f 1773 _d 1774 _s 1775 _g 1776 _t 1777 _v 1778 _s 1779 _k 1780 _ 
                   
               
               
                 t 1781  s 1782 _d 1783 _w 1784 _k 1785 _c 1786 _k 1787 _v 1788 _t 1789 _d 1790 _v 1791 _l 1792 _f 1793 _s 1794 _g 1795 _q 1796 _k 1797   
               
               
                   
               
               
                 (SEQ ID NO: 93) 
                   
               
               
                 k 1986 _c 1987 _k 1988 _g 1989 _l 1990 _n 1991 _k 1992 _p 1993 _f 1994 _v 1995 _k 1996 _d 1997 _n 1998 _v 1999 _s 2000 _f 2001 _v 2002 _a 2003 _d 2004   
                   
               
               
                 d 2005 _s 2006 _g 2007 _t 2008 _p 2009 _v 2010 _v 2011 _e 2012 _y 2013 _l 2014 _s 2015 _k 2016 _e 2017 _d 2018 _l 2019 _h 2020   
               
               
                   
               
               
                 (SEQ ID NO: 94) 
                   
               
               
                 k 1988 _g 1989 _l 1990 _n 1991 _k 1992 _p 1993 _f 1994 _v 1995 _k 1996 _d 1997 _n 1998 _v 1999 _s 2000 _f 2001 _v 2002 _a 2003 _d 2004 _d 2005 _s 2006   
                   
               
               
                 g 2007 _t 2008 _p 2009 _v 2010 _v 2011 _e 2012 _y 2013 _l 2014 _s 2015 _k 2016 _e 2017 _d 2018 _l 2019 _h 2020   
               
               
                   
               
               
                 (SEQ ID NO: 95) 
                   
               
               
                 h 2020 _t 2021 _l 2022 _y 2023 _v 2024 _d 2025 _p 2026 _k 2027 _y 2028 _q 2029 _v 2030 _i 2031 _v 2032 _l 2033 _k 2034   
                   
               
               
                   
               
               
                 (SEQ ID NO: 96) 
                   
               
               
                 k 2027 _y 2028 _q 2029 _v 2030 _i 2031 _v 2032 _l 2033 _k 2034 _d 2035 _n 2036 _v 2037 _l 2038 _s 2039 _s 2040 _m 2041 _l 2042 _r 2043 _l 2044 _h 2045   
                   
               
               
                   
               
               
                 (SEQ ID NO: 97) 
                   
               
               
                 h 2045 _t 2046 _v 2047 _e 2048 _s 2049 _g 2050 _d 2051 _i 2052 _n 2053 _v 2054 _v 2055 _a 2056 _a 2057 _s 2058 _g 2059 _s 2060 _l 2061 _t 2062 _r 2063 _ 
                   
               
               
                 k 2064  v 2065 _k 2066 _l 2067 _l 2068 _f 2069 _r 2070 _a 2071 _s 2072 _f 2073 _y 2074 _f 2075 _k 2076   
               
               
                   
               
               
                 (SEQ ID NO: 98) 
                   
               
               
                 k 2066 _l 2067 _l 2068 _f 2069 _r 2070 _a 2071 _s 2072 _f 2073 _y 2074 _f 2075 _k 2076 _e 2077 _f 2078 _a 2079 _t 2080 _r 2081 _t 2082 _f 2083 _t 2084 _ 
                   
               
               
                 a 2085  t 2086 _t 2087 _a 2088 _v 2089 _g 2090 _s 2091 _c 2092 _i 2093 _k 2094 _s 2095 _v 2096 _v 2097 _r 2098 _h 2099   
               
               
                   
               
               
                 (SEQ ID NO: 10) 
                   
               
               
                 k 2094 _s 2095 _v 2096 _v 2097 _r 2098 _h 2099 _l 2100 _g 2101 _v 2102 _t 2103 _k 2104   
                   
               
               
                   
               
               
                 (SEQ ID NO: 99) 
                   
               
               
                 h 2099 _l 2100 _g 2101 _v 2102 _t 2103 _k 2104 _g 2105 _i 2106 _l 2107 _t 2108 _g 2109 _c 2110 _f 2111 _s 2112 _f 2113 _v 2114 _k 2115 _m 2116 _l 2117 _ 
                   
               
               
                 f 2118  m 2119 _l 2120 _p 2121 _l 2122 _a 2123 _y 2124 _f 2125 _s 2126 _d 2127 _s 2128 _k 2129 _l 2130 _g 2131 _t 2132 _t 2133 _e 2134 _v 2135 _k 2136   
               
               
                   
               
               
                 (SEQ ID NO: 100) 
                   
               
               
                 k 2165 _l 2166 _r 2167 _r 2168 _v 2169 _d 2170 _w 2171 _k 2172 _s 2173 _t 2174 _l 2175 _r 2176 _l 2177 _l 2178 _l 2179 _m 2180 _l 2181 _c 2182 _t 2183 _ 
                   
               
               
                 t 2184  m 2185 _v 2186 _l 2187 _l 2188 _s 2189 _s 2190 _v 2191 _y 2192 _h 2193   
               
               
                   
               
               
                 (SEQ ID NO: 101) 
                   
               
               
                 h 2343 _i 2344 _p 2345 _m 2346 _a 2347 _g 2348 _l 2349 _v 2350 _r 2351 _m 2352 _y 2353 _n 2354 _l 2355 _l 2356 _a 2357 _c 2358 _l 2359 _w 2360 _l 2361 _ 
                   
               
               
                 t 2362  r 2363 _k 2364 _f 2365 _y 2366 _q 2367 _h 2368 _v 2369 _i 2370 _n 2371 _g 2372 _c 2373 _k 2374   
               
               
                   
               
               
                 (SEQ ID NO: 102) 
                   
               
               
                 h 2343 _i 2344 _p 2345 _m 2346 _a 2347 _g 2348 _l 2349 _v 2350 _r 2351 _m 2352 _y 2353 _n 2354 _l 2355 _l 2356 _a 2357 _c 2358 _l 2359 _w 2360 _l 2361 _ 
                   
               
               
                 t 2362  r 2363 _k 2364 _f 2365 _y 2366 _q 2367 _h 2368 _v 2369 _i 2370 _n 2371 _g 2372 _c 2373 _k 2374 _d 2375 _t 2376 _a 2377 _c 2378 _l 2379 _l 2380 _ 
               
               
                 c 2381 _y 2382  k 2383   
               
               
                   
               
               
                 Mid-molecule 
               
               
                 (SEQ ID NO: 11) 
                   
               
               
                 k 2364 _f 2365 _y 2366 _q 2367 _h 2368 _v 2369 _i 2370 _n 2371 _g 2372 _c 2373 _k 2374   
                   
               
               
                   
               
               
                 (SEQ ID NO: 103) 
                   
               
               
                 h 2368 _v 2369 _i 2370 _n 2371 _g 2372 _c 2373 _k 2374 _d 2375 _t 2376 _a 2377 _c 2378 _l 2379 _l 2380 _c 2381 _y 2382 _k 2383   
                   
               
               
                   
               
               
                 (SEQ ID NO: 104) 
                   
               
               
                 k 2374 _d 2375 _t 2376 _a 2377 _c 2378 _l 2379 _l 2380 _c 2381 _y 2382 _k 2383 _r 2384 _n 2385 _r 2386 _l 2387 _t 2388 _r 2389 _v 2390 _e 2391 _a 2392 _ 
                   
               
               
                 s 2393  t 2394 _v 2395 _v 2396 _c 2397 _g 2398 _g 2399 _k 2400 _r 2401 _t 2402 _f 2403 _y 2404 _i 2405 _t 2406 _a 2407 _n 2408 _g 2409 _g 2410 _i 2411 _ 
               
               
                 s 2412 _f 2413  c 2414 _r 2415 _r 2416 _h 2417   
               
               
                   
               
               
                 (SEQ ID NO: 105) 
                   
               
               
                 h 2457 _y 2458 _y 2459 _v 2460 _d 2461 _s 2462 _v 2463 _t 2464 _v 2465 _k 2466 _e 2467 _t 2468 _v 2469 _v 2470 _q 2471 _f 2472 _n 2473 _y 2474 _r 2475   
                   
               
               
                 r 2476 _d 2477 _g 2478 _q 2479 _p 2480 _f 2481 _y 2482 _e 2483 _r 2484 _f 2485 _p 2486 _l 2487 _c 2488 _a 2489 _f 2490 _t 2491 _n 2492 _l 2493 _d 2494 _ 
               
               
                 k 2495  l 2496 _k 2497 _f 2498 _k 2499 _e 2500 _v 2501 _c 2502 _k 2503   
               
               
                   
               
               
                 (SEQ ID NO: 106) 
                   
               
               
                 h 3109 _v 3110 _s 3111 _w 3112 _y 3113 _i 3114 _m 3115 _f 3116 _g 3117 _p 3118 _i 3119 _v 3120 _p 3121 _i 3122 _w 3123 _m 3124 _t 3125 _c 3126 _v 3127   
                   
               
               
                 y 3128 _t 3129 _v 3130 _a 3131 _m 3132 _c 3133 _f 3134 _r 3135 _h 3136 _f 3137 _f 3138 _w 3139 _v 3140 _l 3141 _a 3142 _y 3143 _f 3144 _s 3145 _k 3146 _ 
               
               
                 k 3147  h 3148 _v 3149 _e 3150 _v 3151 _f 3152 _t 3153 _d 3154 _g 3155 _k 3156   
               
               
                   
               
               
                 (SEQ ID NO: 107) 
                   
               
               
                 h 3136 _f 3137 _f 3138 _w 3139 _v 3140 _l 3141 _a 3142 _y 3143 _f 3144 _s 3145 _k 3146 _k 3147 _h 3148 _v 3149 _e 3150 _v 3151 _f 3152 _t 3153 _d 3154 _ 
                   
               
               
                 g 3155  k 3156   
               
               
                   
               
               
                 (SEQ ID NO: 108) 
                   
               
               
                 h 3136 _f 3137 _f 3138 _w 3139 _v 3140 _l 3141 _a 3142 _y 3143 _f 3144 _s 3145 _k 3146 _k 3147 h 3148 _v 3149 _e 3150 _v 3151 _f 3152 _t 3153 _d 3154 _ 
                   
               
               
                 g 3155 _k 3156   
               
               
                   
               
               
                 (SEQ ID NO: 6) 
                   
               
               
                 k 3147 h 3148 _v 3149 _e 3150 _v 3151 _f 3152 _t 3153 _d 3154 _g 3155 _k 3156   
                   
               
               
                   
               
               
                 (SEQ ID NO: 108) 
                   
               
               
                 h 3422 _t 3423 _g 3424 _s 3425 _a 3426 _f 3427 _d 3428 _g 3429 _t 3430 _m 3431 _y 3432 _g 3433 _a 3434 _f 3435 _m 3436 _d 3437 _k 3438 _q 3439 _v 3440   
                   
               
               
                 h 3441 _q 3442 _v 3443 _q 3444 _l 3445 _t 3446 _d 3447 _k 3448   
               
               
                   
               
               
                 (SEQ ID NO: 12) 
                   
               
               
                 d 3437 _k 3438 _q 3439 _v 3440 _h 3441 _q 3442 _v 3443 _q 3444 _l 3445 _t 3446 _d 3447 _k 3448   
                   
               
               
                   
               
               
                 (SEQ ID NO: 109) 
                   
               
               
                 k 3840 _v 3841 _a 3842 _a 3843 _m 3844 _q 3845 _s 3846 _k 3847 _l 3848 _t 3849 _d 3850 _l 3851 _k 3852 _c 3853 _t 3854 _s 3855 _v 3856 _v 3857 _l 3858 _ 
                   
               
               
                 l 3859  s 3860 _v 3861 _l 3862 _q 3863 _q 3864 _l 3865 _h 3866 _l 3867 _e 3868 _a 3869 _n 3870 _s 3871 _r 3872 _a 3873 _w 3874 _a 3875 _f 3876 _c 3877 _ 
               
               
                 v 3878 _k 3879  c 3880 _h 3881   
               
               
                   
               
               
                 (SEQ ID NO: 110) 
                   
               
               
                 k 3840 _v 3841 _a 3842 _a 3843 _m 3844 _q 3845 _s 3846 _k 3847 _l 3848 _t 3849 _d 3850 _l 3851 _k 3852 _c 3853 _t 3854 _s 3855 _v 3856 _v 3857 _l 3858 _ 
                   
               
               
                 l 3859  s 3860 _v 3861 _l 3862 _q 3863 _q 3864 _l 3865 _h 3866   
               
               
                   
               
               
                 (SEQ ID NO: 111) 
                   
               
               
                 h 3936 _l 3937 _a 3938 _t 3939 _f 3940 _a 3941 _e 3942 _l 3943 _e 3944 _a 3945 _a 3946 _q 3947 _k 3948 _a 3949 _y 3950 _q 3951 _e 3952 _a 3953 _m 3954   
                   
               
               
                 d 3955 _s 3956 _g 3957 _d 3958 _t 3959 _s 3960 _p 3961 _q 3962 _v 3963 _l 3964 _k 3965 _a 3966 _l 3967 _q 3968 _k 3969 _a 3970 _v 3971 _n 3972 _i 3973 _ 
               
               
                 a 3974  k 3975   
               
               
                   
               
               
                 (SEQ ID NO: 112) 
                   
               
               
                 h 3936 _l 3937 _a 3938 _t 3939 _f 3940 _a 3941 _e 3942 _l 3943 _e 3944 _a 3945 _a 3946 _q 3947 _k 3948 _a 3949 _y 3950 _q 3951 _e 3952 _a 3953 _m 3954   
                   
               
               
                 d 3955 _s 3956 _g 3957 _d 3958 _t 3959 _s 3960 _p 3961 _q 3962 _v 3963 _l 3964 _k 3965 _a 3966 _l 3967 _q 3968 _k 3969 _a 3970 _v 3971 _n 3972 _i 3973 _ 
               
               
                 a 3974  k 3975 _n 3976 _a 3977 _y 3978 _e 3979 _k 3980 _d 3981 _k 3982   
               
               
                   
               
               
                 (SEQ ID NO: 113) 
                   
               
               
                 k 4178 _w 4179 _a 4180 _r 4181 _v 4182 _e 4183 _g 4184 _k 4185 _d 4186 _g 4187 _f 4188 _v 4189 _s 4190 _v 4191 _e 4192 _l 4193 _q 4194 _p 4195 _p 4196   
                   
               
               
                 c 4197 _k 4198 _f 4199 _l 4200 _i 4201 _a 4202 _g 4203 _p 4204 _k 4205 _g 4206 _p 4207 _e 4208 _i 4209 _r 4210 _y 4211 _l 4212 _y 4213 _f 4214 _v 4215 _ 
               
               
                 k 4216  n 4217 _l 4218 _n 4219 _n 4220 _l 4221 _h 4222   
               
               
                   
               
               
                 (SEQ ID NO: 114) 
                   
               
               
                 k 4198 _f 4199 _l 4200 _i 4201 _a 4202 _g 4203 _p 4204 _k 4205 _g 4206 _p 4207 _e 4208 _i 4209 _r 4210 _y 4211 _l 4212 _y 4213 _f 4214 _v 4215 _k 4216 _ 
                   
               
               
                 n 4217  l 4218 _n 4219 _n 4220 _l 4221 _h 4222   
               
               
                   
               
               
                 (SEQ ID NO: 115) 
                   
               
               
                 k 4198 _f 4199 _l 4200 _i 4201 _a 4202 _g 4203 _p 4204 _k 4205 _g 4206 _p 4207 _e 4208 _i 4209 _r 4210 _y 4211 _l 4212 _y 4213 _f 4214 _v 4215 _k 4216 _ 
                   
               
               
                 n 4217  l 4218 _n 4219 _n 4220 _l 4221 _h 4222 _r 4223 _g 4224 _q 4225 _v 4226 _l 4227 _g 4228 _h 4229   
               
               
                   
               
               
                 (SEQ ID NO: 116) 
                   
               
               
                 k 4285 _t 4286 _g 4287 _t 4288 _g 4289 _i 4290 _a 4291 _i 4292 _s 4293 _v 4294 _k 4295 _p 4296 _e 4297 _s 4298 _t 4299 _a 4300 _d 4301 _q 4302 _e 4303 _ 
                   
               
               
                 t 4304  y 4305 _g 4306 _g 4307 _a 4308 _s 4309 _v 4310 _c 4311 _l 4312 _y 4313 _c 4314 _r 4315 _a 4316 _h 4317   
               
               
                   
               
               
                 (SEQ ID NO: 117) 
                   
               
               
                 k 4418 _a 4419 _k 4420 _v 4421 _a 4422 _g 4423 _i 4424 _g 4425 _k 4426 _y 4427 _y 4428 _k 4429 _t 4430 _n 4431 _t 4432 _c 4433 _r 4434 _f 4435 _v 4436 _ 
                   
               
               
                 e 4437  l 4438 _d 4439 _d 4440 _q 4441 _g 4442 _h 4443   
               
               
                   
               
               
                 (SEQ ID NO: 118) 
                   
               
               
                 k 4418 _a 4419 _k 4420 _v 4421 _a 4422 _g 4423 _i 4424 _g 4425 _k 4426 _y 4427 _y 4428 _k 4429 _t 4430 _n 4431 _t 4432 _c 4433 _r 4434 _f 4435 _v 4436 _ 
                   
               
               
                 e 4437  l 4438 _d 4439 _d 4440 _q 4441 _g 4442 _h 4443 _h 4444   
               
               
                   
               
               
                 (SEQ ID NO: 119) 
                   
               
               
                 k 4418 _a 4419 _k 4420 _v 4421 _a 4422 _g 4423 _i 4424 _g 4425 _k 4426 _y 4427 _y 4428 _k 4429 _t 4430 _n 4431 _t 4432 _c 4433 _r 4434 _f 4435 _v 4436 _ 
                   
               
               
                 e 4437  l 4438 _d 4439 _d 4440 _q 4441 _g 4442 _h 4443 _h 4444 _l 4445 _d 4446 _s 4447 _y 4448 _f 4449 _v 4450 _v 4451 _k 4452 _r 4453 _h 4454   
               
               
                   
               
               
                 (SEQ ID NO: 120) 
                   
               
               
                 k 4418 _a 4419 _k 4420 _v 4421 _a 4422 _g 4423 _i 4424 _g 4425 _k 4426 _y 4427 _y 4428 _k 4429 _t 4430 _n 4431 _t 4432 _c 4433 _r 4434 _f 4435 _v 4436 _ 
                   
               
               
                 e 4437  l 4438 _d 4439 _d 4440 _q 4441 _g 4442 _h 4443 _h 4444 _l 4445 _d 4446 _s 4447 _y 4448 _f 4449 _v 4450 _v 4451 _k 4452 _r 4453 _h 4454 _ 
               
               
                 t 4455 _m 4456  e 4457 _n 4458 _y 4459 _e 4460 _l 4461 _e 4462 _k 4463 _h 4464   
               
               
                   
               
               
                 (SEQ ID NO: 121) 
                   
               
               
                 k 4420 _v 4421 _a 4422 _g 4423 _i 4424 _g 4425 _k 4426 _y 4427 _y 4428 _k 4429 _t 4430 _n 4431 _t 4432 _c 4433 _r 4434 _f 4435 _v 4436 _e 4437 _l 4438 _ 
                   
               
               
                 d 4439  d 4440 _q 4441 _g 4442 _h 4443 _h 4444 _l 4445 _d 4446 _s 4447 _y 4448 _f 4449 _v 4450 _v 4451 _k 4452 _r 4453 _h 4454   
               
               
                   
               
               
                 (SEQ ID NO: 122) 
                   
               
               
                 k 4420 _v 4421 _a 4422 _g 4423 _i 4424 _g 4425 _k 4426 _y 4427 _y 4428 _k 4429 _t 4430 _n 4431 _t 4432 _c 4433 _r 4434 _f 4435 _v 4436 _e 4437 _l 4438 _ 
                   
               
               
                 d 4439  d 4440 _q 4441 _g 4442 _h 4443 _h 4444 _l 4445 _d 4446 _s 4447 _y 4448 _f 4449 _v 4450 _v 4451 _k 4452 _r 4453 _h 4454 _t 4455 _m 4456 _e 4457 _ 
               
               
                 n 4458  y 4459 _e 4460 _l 4461 _e 4462 _k 4463 _h 4464   
               
               
                   
               
               
                 (SEQ ID NO: 123) 
                   
               
               
                 k 4420 _v 4421 _a 4422 _g 4423 _i 4424 _g 4425 _k 4426 _y 4427 _y 4428 _k 4429 _t 4430 _n 4431 _t 4432 _c 4433 _r 4434 _f 4435 _v 4436 _e 4437 _l 4438 _ 
                   
               
               
                 d 4439  d 4440 _q 4441 _g 4442 _h 4443   
               
               
                   
               
               
                 (SEQ ID NO: 124) 
                   
               
               
                 k 4420 _v 4421 _a 4422 _g 4423 _i 4424 _g 4425 _k 4426 _y 4427 _y 4428 _k 4429 _t 4430 _n 4431 _t 4432 _c 4433 _r 4434 _f 4435 _v 4436 _e 4437 _l 4438 _ 
                   
               
               
                 d 4439  d 4440 _q 4441 _g 4442 _h 4443 _h 4444   
               
               
                   
               
               
                 (SEQ ID NO: 125) 
                   
               
               
                 h 4554 _k 4555 _l 4556 _g 4557 _e 4558 _r 4559 _v 4560 _r 4561 _q 4562 _a 4563 _i 4564 _l 4565 _n 4566 _l 4567 _t 4568 _k 4569 _f 4570 _c 4571 _d 4572 _ 
                   
               
               
                 h 4573  m 4574 _v 4575 _k 4576   
               
               
                   
               
               
                 (SEQ ID NO: 20) 
                   
               
               
                 k 4569 _f 4570 _c 4571 _d 4572 _h 4573 _m 4574 _v 4575 _k 4576   
                   
               
               
                   
               
               
                 (SEQ ID NO: 126) 
                   
               
               
                 h 4634 _r 4635 _d 4636 _c 4637 _d 4638 _f 4639 _n 4640 _k 4641 _p 4642 _l 4643 _i 4644 _e 4645 _w 4646 _p 4647 _l 4648 _t 4649 _e 4650 _y 4651 _d 4652 _ 
                   
               
               
                 f 4653  t 4654 _d 4655 _y 4656 _k 4657 _v 4658 _q 4659 _l 4660 _f 4661 _e 4662 _k 4663   
               
               
                   
               
               
                 (SEQ ID NO: 127) 
                   
               
               
                 h 4634 _r 4635 _d 4636 _c 4637 _d 4638 _f 4639 _n 4640 _k 4641 _p 4642 _l 4643 _i 4644 _e 4645 _w 4646 _p 4647 _l 4648 _t 4649 _e 4650 _y 4651 _d 4652 _ 
                   
               
               
                 f 4653  t 4654 _d 4655 _y 4656 _k 4657 _v 4658 _q 4659 _l 4660 _f 4661 _e 4662 _k 4663 _y 4664 _f 4665 _k 4666   
               
               
                   
               
               
                 (SEQ ID NO: 128) 
                   
               
               
                 k 4657 _v 4658 _q 4659 _l 4660 _f 4661 _e 4662 _k 4663 _y 4664 _f 4665 _k 4666 _y 4667 _w 4668 _d 4669 _q 4670 _t 4671 _y 4672 _h 4673   
                   
               
               
                   
               
               
                 (SEQ ID NO: 129) 
                   
               
               
                 k 4657 _v 4658 _q 4659 _l 4660 _f 4661 _e 4662 _k 4663 _y 4664 _f 4665 _k 4666 _y 4667 _w 4668 _d 4669 _q 4670 _t 4671 _y 4672 _h 4673 _a 4674 _n 4675   
                   
               
               
                 c 4676 _v 4677 _n 4678 _c 4679 _t 4680 _d 4681 _d 4682 _r 4683 _c 4684 _v 4685 _l 4686 _h 4687   
               
               
                   
               
               
                 (SEQ ID NO: 130) 
                   
               
               
                 h 4687 _c 4688 _a 4689 _n 4690 _f 4691 _n 4692 _v 4693 _l 4694 _f 4695 _a 4696 _m 4697 _t 4698 _m 4699 _p 4700 _k 4701 _t 4702 _c 4703 _f 4704 _g 4705   
                   
               
               
                 p 4706 _i 4707 _v 4708 _r 4709 _k 4710   
               
               
                   
               
               
                 (SEQ ID NO: 131) 
                   
               
               
                 k 4701 _t 4702 _c 4703 _f 4704 _g 4705 _p 4706 _i 4707 _v 4708 _r 4709 _k 4710 _i 4711 _f 4712 _v 4713 _d 4714 _g 4715 _v 4716 _p 4717 _f 4718 _v 4719 _ 
                   
               
               
                 v 4720  s 4721 _c 4722 _g 4723 _y 4724 _h 4725   
               
               
                   
               
               
                 (SEQ ID NO: 132) 
                   
               
               
                 k 4701 _t 4702 _c 4703 _f 4704 _g 4705 _p 4706 _i 4707 _v 4708 _r 4709 _k 4710 _i 4711 _f 4712 _v 4713 _d 4714 _g 4715 _v 4716 _p 4717 _f 4718 _v 4719 _ 
                   
               
               
                 v 4720  s 4721 _c 4722 _g 4723 _y 4724 _h 4725 _y 4726 _k 4727 _e 4728 _l 4729 _g 4730 _l 4731 _v 4732 _m 4733 _n 4734 _m 4735 _d 4736 _v 4737 _s 4738 _ 
               
               
                 l 4739 _h 4740   
               
               
                   
               
               
                 (SEQ ID NO: 133) 
                   
               
               
                 k 4701 _t 4702 _c 4703 _f 4704 _g 4705 _p 4706 _i 4707 _v 4708 _r 4709 _k 4710 _i 4711 _f 4712 _v 4713 _d 4714 _g 4715 _v 4716 _p 4717 _f 4718 _v 4719 _ 
                   
               
               
                 v 4720  s 4721 _c 4722 _g 4723 _y 4724 _h 4725 _y 4726 _k 4727 _e 4728 _l 4729 _g 4730 _l 4731 _v 4732 _m 4733 _n 4734 _m 4735 _d 4736 _v 4737 _s 4738 _ 
               
               
                 l 4739  h 4740 _r 4741 _h 4742   
               
               
                   
               
               
                 Carboxy-terminal 
               
               
                 (SEQ ID NO: 28) 
                   
               
               
                 k 4808 _e 4809 _g 4810 _s 4811 _s 4812 _v 4813 _t 4814 _l 4815 _k 4816 _h 4817   
                   
               
               
                   
               
               
                 (SEQ ID NO: 2) 
                   
               
               
                 k 4878 _s 4879 _a 4880 _g 4881 _h 4882 _p 4883 _f 4884 _n 4885 _k 4886   
                   
               
               
                   
               
               
                 (SEQ ID NO: 134) 
                   
               
               
                 h 4882 _p 4883 _f 4884 _n 4885 _k 4886 _f 4887 _g 4888 _k 4889 _a 4890 _r 4891 _v 4892 _v 4893 _y 4894 _e 4895 _s 4896 _m 4897 _s 4898 _y 4899 _q 4900   
                   
               
               
                 e 4901 _q 4902 _d 4903 _e 4904 _l 4905 _f 4906 _a 4907 _m 4908 _t 4909 _k 4910 _r 4911 _n 4912 _v 4913 _i 4914 _p 4915 _t 4916 _m 4917 _t 4918 _q 4919   
               
               
                 m 4920 _n 4921 _l 4922 _k 4923 _y 4924 _a 4925 _i 4926 _s 4927 _a 4928 _k 4929   
               
               
                   
               
               
                 (SEQ ID NO: 135) 
                   
               
               
                 k 4923 _y 4924 _a 4925 _i 4926 _s 4927 _a 4928 _k 4929 _n 4930 _r 4931 _a 4932 _r 4933 _t 4934 _v 4935 _a 4936 _g 4937 _v 4938 _s 4939 _i 4940 _l 4941 _ 
                   
               
               
                 s 4942  t 4943 _m 4944 _t 4945 _n 4946 _r 4947 _q 4948 _y 4949 _h 4950   
               
               
                   
               
               
                 (SEQ ID NO: 136) 
                   
               
               
                 h 4950 _q 4951 _k 4952 _m 4953 _l 4954 _k 4955 _s 4956 _m 4957 _a 4958 _a 4959 _t 4960 _r 4961 _g 4962 _a 4963 _t 4964 _c 4965 _v 4966 _i 4967 _g 4968   
                   
               
               
                 t 4969 _t 4970 _k 4971 _f 4972 _y 4973 _g 4974 _g 4975 _w 4976 _d 4977 _f 4978 _m 4979 _l 4980 _k 4981   
               
               
                   
               
               
                 (SEQ ID NO: 137) 
                   
               
               
                 k 4971 _f 4972 _y 4973 _g 4974 _g 4975 _w 4976 _d 4977 _f 4978 _m 4979 _l 4980 _k 4981 _t 4982 _l 4983 _y 4984 _k 4985 _d 4986 _v 4987 _d 4988 _n 4989   
                   
               
               
                 p 4990 _h 4991   
               
               
                   
               
               
                 (SEQ ID NO: 138) 
                   
               
               
                 k 4971 _f 4972 _y 4973 _g 4974 _g 4975 _w 4976 _d 4977 _f 4978 _m 4979 _l 4980 _k 4981 _t 4982 _l 4983 _y 4984 _k 4985 _d 4986 _v 4987 _d 4988 _n 4989   
                   
               
               
                 p 4990 _h 4991 _l 4992 _m 4993 _g 4994 _w 4995 _d 4996 _y 4997 _p 4998 _k 4999 _c 5000 _d 5001 _r 5002 _a 5003 _m 5004 _p 5005 _n 5006 _m 5007 _c 5008   
               
               
                 r 5009 _i 5010 _f 5011 _a 5012 _s 5013 _l 5014 _i 5015 _l 5016 _a 5017 _r 5018 _k 5019 _h 5020   
               
               
                   
               
               
                 (SEQ ID NO: 139) 
                   
               
               
                 k 5092 _i 5093 _v 5094 _d 5095 _k 5096 _e 5097 _v 5098 _k 5099 _d 5100 _m 5101 _q 5102 _f 5103 _d 5104 _l 5105 _y 5106 _v 5107 _n 5108 _v 5109 _y 5110   
                   
               
               
                 r 5111 _s 5112 _t 5113 _s 5114 _p 5115 _d 5116 _p 5117 _k 5118 _f 5119 _v 5120 _d 5121 _k 5122 _y 5123 _y 5124 _a 5125 _f 5126 _l 5127 _n 5128 _k 5129   
               
               
                 h 5130   
               
               
                   
               
               
                 (SEQ ID NO: 17) 
                   
               
               
                 k 5122 _y 5123 _y 5124 _a 5125 _f 5126 _l 5127 _n 5128 _k 5129 _h 5130   
                   
               
               
                   
               
               
                 (SEQ ID NO: 140) 
                   
               
               
                 h 5130 _f 5131 _s 5132 _m 5133 _m 5134 _i 5135 _l 5136 _s 5137 _d 5138 _d 5139 _g 5140 _v 5141 _v 5142 _c 5143 _y 5144 _n 5145 _s 5146 _d 5147 _y 5148   
                   
               
               
                 a 5149 _a 5150 _k 5151 _g 5152 _y 5153 _i 5154 _a 5155 _g 5156 _i 5157 _q 5158 _n 5159 _f 5160 _k 5161   
               
               
                   
               
               
                 (SEQ ID NO: 141) 
                   
               
               
                 g 5152 _y 5153 _i 5154 _a 5155 _g 5156 _i 5157 _q 5158 _n 5159 _f 5160 _k 5161 _e 5162 _t 5163 _l 5164 _y 5165 _y 5166 _q 5167 _n 5168 _n 5169 _v 5170   
                   
               
               
                 f 5171 _m 5172 _s 5173 _e 5174 _a 5175 _k 5176 _c 5177 _w 5178 _v 5179 _e 5180 _t 5181 _d 5182 _l 5183 _k 5184 _k 5185 _g 5186 _p 5187 _h 5188   
               
               
                   
               
               
                 (SEQ ID NO: 142) 
                   
               
               
                 g 5152 _y 5153 _i 5154 _a 5155 _g 5156 _i 5157 _q 5158 _n 5159 _f 5160 _k 5161 _e 5162 _t 5163 _l 5164 _y 5165 _y 5166 _q 5167 _n 5168 _n 5169 _v 5170   
                   
               
               
                 f 5171 _m 5172 _s 5173 _e 5174 _a 5175 _k 5176 _c 5177 _w 5178 _v 5179 _e 5180 _t 5181 _d 5182 _l 5183 _k 5184 _k 5185 _g 5186 _p 5187 _h 5188 _e 5189   
               
               
                 f 5190 _c 5191 _s 5192 _q 5193 _h 5914   
               
               
                   
               
               
                 (SEQ ID NO: 143) 
                   
               
               
                 k 5176 _c 5177 _w 5178 _v 5179 _e 5180 _t 5181 _d 5182 _l 5183 _k 5184 _k 5185 _g 5186 _p 5187 _h 5188   
                   
               
               
                   
               
               
                 (SEQ ID NO: 144) 
                   
               
               
                 k 5176 _c 5177 _w 5178 _v 5179 _e 5180 _t 5181 _d 5182 _l 5183 _k 5184 _k 5185 _g 5186 _p 5187 _h 5188 _e 5189 _f 5190 _c 5191 _s 5192 _q 5193 _h 5914   
                   
               
               
                   
               
               
                 (SEQ ID NO: 145) 
                   
               
               
                 k 5456 _q 5457 _s 5458 _y 5459 _a 5460 _i 5461 _a 5462 _t 5463 _i 5464 _k 5465 _e 5466 _i 5467 _v 5468 _g 5469 _e 5470 _r 5471 _q 5472 _l 5473 _l 5474 _ 
                   
               
               
                 l 5475  v 5476 _w 5477 _e 5478 _a 5479 _g 5480 _k 5481 _s 5482 _k 5483 _p 5484 _p 5485 _l 5486 _n 5487 _r 5488 _n 5489 _y 5490 _v 5491 _f 5492 _t 5493 _ 
               
               
                 g 5494  y 5495 _h 5496   
               
               
                   
               
               
                 (SEQ ID NO: 146) 
                   
               
               
                 h 5496 _i 5497 _t 5498 _k 5499 _n 5500 _s 5501 _k 5502 _v 5503 _q 5504 _l 5505 _g 5506 _e 5507 _y 5508 _i 5509 _f 5510 _e 5511 _r 5512 _i 5513 _d 5514 _ 
                   
               
               
                 y 5515  s 5516 _d 5517 _a 5518 _v 5519 _s 5520 _y 5521 _k 5522 _s 5523 _s 5524 _t 5525 _t 5526 _y 5527 _k 5528   
               
               
                   
               
               
                 (SEQ ID NO: 147) 
                   
               
               
                 k 5522 _s 5523 _s 5524 _t 5525 _t 5526 _y 5527 _k 5528 _l 5529 _t 5530 _v 5531 _g 5532 _d 5533 _i 5534 _f 5535 _v 5536 _l 5537 _t 5538 _s 5539 _h 5540   
                   
               
               
                   
               
               
                 (SEQ ID NO: 148) 
                   
               
               
                 h 5600 _f 5601 _a 5602 _i 5603 _g 5604 _l 5605 _a 5606 _i 5607 _y 5608 _y 5609 _p 5610 _t 5611 _a 5612 _r 5613 _v 5614 _v 5615 _y 5616 _t 5617 _a 5618 _ 
                   
               
               
                 c 5619  s 5620 _h 5621 _a 5622 _a 5623 _v 5624 _d 5625 _a 5626 _l 5627 _c 5628 _e 5629 _k 5630 _a 5631 _f 5632 _k 5633 _y 5634 _l 5635 _n 5636 _i 5637 _ 
               
               
                 a 5638 _k 5639   
               
               
                   
               
               
                 (SEQ ID NO: 149) 
                   
               
               
                 h 5600 _f 5601 _a 5602 _i 5603 _g 5604 _l 5605 _a 5606 _i 5607 _y 5608 _y 5609 _p 5610 _t 5611 _a 5612 _r 5613 _v 5614 _v 5615 _y 5616 _t 5617 _a 5618 _ 
                   
               
               
                 c 5619  s 5620 _h 5621 _a 5622 _a 5623 _v 5624 _d 5625 _a 5626 _l 5627 _c 5628 _e 5629 _k 5630 _a 5631 _f 5632 _k 5633 _y 5634 _l 5635 _n 5636 _i 5637 _ 
               
               
                 a 5638 _k 5639 c 5640 _s 5641 _r 5642 _i 5643 _i 5644 _p 5645 _a 5646 _k 5647   
               
               
                   
               
               
                 (SEQ ID NO: 150) 
                   
               
               
                 h 5621 _a 5622 _a 5623 _v 5624 _d 5625 _a 5626 _l 5627 _c 5628 _e 5629 _k 5630 _a 5631 _f 5632 _k 5633 _y 5634 _l 5635 _n 5636 _i 5637 _a 5638 _k 5639   
                   
               
               
                   
               
               
                 (SEQ ID NO: 151) 
                   
               
               
                 h 5621 _a 5622 _a 5623 _v 5624 _d 5625 _a 5626 _l 5627 _c 5628 _e 5629 _k 5630 _a 5631 _f 5632 _k 5633 _y 5634 _l 5635 _n 5636 _i 5637 _a 5638 _k 5639 _ 
                   
               
               
                 c 5640  s 5641 _r 5642 _i 5643 _i 5644 _p 5645 _a 5646 _k 5647   
               
               
                   
               
               
                 (SEQ ID NO: 152) 
                   
               
               
                 h 5621 _a 5622 _a 5623 _v 5624 _d 5625 _a 5626 _l 5627 _c 5628 _e 5629 _k 5630 _a 5631 _f 5632 _k 5633 _y 5634 _l 5635 _n 5636 _i 5637 _a 5638 _k 5639 _ 
                   
               
               
                 c 5640  s 5641 _r 5642 _i 5643 _i 5644 _p 5645 _a 5646 _k 5647 _a 5648 _r 5649 _v 5650 _e 5651 _c 5652 _y 5653 _d 5654 _r 5655 _f 5656 _k 5657   
               
               
                   
               
               
                 (SEQ ID NO: 153) 
                   
               
               
                 k 5774 _k 5775 _e 5776 _l 5777 _s 5778 _g 5779 _q 5780 _c 5781 _f 5782 _k 5783 _i 5784 _l 5785 _y 5786 _k 5787 _g 5788 _n 5789 _v 5790 _t 5791 _h 5792   
                   
               
               
                   
               
               
                 (SEQ ID NO: 154) 
                   
               
               
                 k 5775 _e 5776 _l 5777 _s 5778 _g 5779 _q 5780 _c 5781 _f 5782 _k 5783 _i 5784 _l 5785 _y 5786 _k 5787 _g 5788 _n 5789 _v 5790 _t 5791 _h 5792   
                   
               
               
                   
               
               
                 (SEQ ID NO: 16) 
                   
               
               
                 k 6055 _h 6056 _l 6057 _v 6058 _p 6059 _l 6060 _m 6061 _h 6062 _k 6063   
                   
               
               
                   
               
               
                 (SEQ ID NO: 155) 
                   
               
               
                 k 6055 _h 6056 _l 6057 _v 6058 _p 6059 _l 6060 _m 6061 _h 6062 _k 6063 _g 6064 _a 6065 _a 6066 _w 6067 _p 6068 _i 6069 _v 6070 _r 6071 _r 6072 _r 6073   
                   
               
               
                 i 6074 _v 6075 _q 6076 _m 6077 _l 6078 _s 6079 _d 6080 _t 6081 _l 6082 _d 6083 _k 6084 _l 6085 _s 6086 _d 6087 _y 6088 _c 6089 _t 6090 _f 6091 _v 6092 _ 
               
               
                 c 6093  w 6094 _a 6095 _h 6096   
               
               
                   
               
               
                 (SEQ ID NO: 156) 
                   
               
               
                 h 6096 _g 6097 _f 6098 _e 6099 _l 6100 _t 6101 _s 6102 _a 6103 _s 6104 _y 6105 _f 6106 _c 6107 _k 6108 _i 6109 _g 6110 _k 6111 _e 6112 _q 6113 _k 6114   
                   
               
               
                   
               
               
                 (SEQ ID NO: 157) 
                   
               
               
                 k 6108 _i 6109 _g 6110 _k 6111 _e 6112 _q 6113 _k 6114 _c 6115 _c 6116 _m 6117 _c 6118 _n 6119 _r 6120 _r 6121 _a 6122 _a 6123 _a 6124 _y 6125 _s 6126   
                   
               
               
                 s 6127 _p 6128 _l 6129 _q 6130 _s 6131 _y 6132 _a 6133 _c 6134 _w 6135 _t 6136 _h 6137   
               
               
                   
               
               
                 (SEQ ID NO: 158) 
                   
               
               
                 h 6209 _e 6210 _k 6211 _k 6212 _l 6213 _n 6214 _s 6215 _c 6216 _c 6217 _r 6218 _i 6219 _v 6220 _e 6221 _r 6222 _n 6223 _v 6224 _v 6225 _r 6226 _a 6227 _ 
                   
               
               
                 a 6228  l 6229 _l 6230 _a 6231 _g 6232 _s 6233 _f 6234 _d 6235 _k 6236 _v 6237 _y 6238 _d 6239 _i 6240 _g 6241 _n 6242 _p 6243 _k 6244   
               
               
                   
               
               
                 (SEQ ID NO: 159) 
                   
               
               
                 k 6236 _v 6237 _y 6238 _d 6239 _i 6240 _g 6241 _n 6242 _p 6243 _k 6244 _g 6245 _i 6246 _p 6247 _i 6248 _v 6249 _d 6250 _d 6251 _p 6252 _v 6253 _v 6254   
                   
               
               
                 d 6255 _w 6256 _h 6257   
               
               
                   
               
               
                 (SEQ ID NO: 160) 
                   
               
               
                 k 6492 _r 6493 _a 6494 _v 6495 _r 6496 _s 6497 _h 6498 _p 6499 _d 6500 _f 6501 _k 6502   
                   
               
               
                   
               
               
                 (SEQ ID NO: 161) 
                   
               
               
                 k 6492 _r 6493 _a 6494 _v 6495 _r 6496 _s 6497 _h 6498 _p 6499 _d 6500 _f 6501 _k 6502 _l 6503 _l 6504 _h 6505   
                   
               
               
                   
               
               
                 (SEQ ID NO: 162) 
                   
               
               
                 h 6678 _l 6679 _l 6680 _i 6681 _g 6682 _l 6683 _y 6684 _k 6685 _k 6686 _q 6687 _q 6688 _e 6689 _g 6690 _h 6691 _i 6692 _i 6693 _m 6694 _e 6695 _e 6696   
                   
               
               
                 m 6697 _l 6698 _k 6699 _g 6700 _s 6701 _s 6702 _t 6703 _i 6704 _h 6705 _n 6706 _y 6707 _f 6708 _i 6709 _t 6710 _e 6711 _t 6712 _n 6713 _t 6714 _a 6715 _ 
               
               
                 a 6716  f 6717 _k 6718 _a 6719 _v 6720 _c 6721 _s 6722 _v 6723 _i 6724 _d 6725 _l 6726 _k 6727   
               
               
                   
               
               
                 (SEQ ID NO: 163) 
                   
               
               
                 h 6691 _i 6692 _i 6693 _m 6694 _e 6695 _e 6696 _m 6697 _l 6698 _k 6699 _g 6700 _s 6701 _s 6702 _t 6703 _i 6704 _h 6705 _n 6706 _y 6707 _f 6708 _i 6709 _ 
                   
               
               
                 t 6710  e 6711 _t 6712 _n 6713 _t 6714 _a 6715 _a 6716 _f 6717 _k 6718 _a 6719 _v 6720 _c 6721 _s 6722 _v 6723 _i 6724 _d 6725 _l 6726 _k 6727   
               
               
                   
               
               
                 (SEQ ID NO: 164) 
                   
               
               
                 h 6691 _i 6692 _i 6693 _m 6694 _e 6695 _e 6696 _m 6697 _l 6698 _k 6699 _g 6700 _s 6701 _s 6702 _t 6703 _i 6704 _h 6705 _n 6706 _y 6707 _f 6708 _i 6709 _ 
                   
               
               
                 t 6710  e 6711 _t 6712 _n 6713 _t 6714 _a 6715 _a 6716 _f 6717 _k 6718 _a 6719 _v 6720 _c 6721 _s 6722 _v 6723 _i 6724 _d 6725 _l 6726 _k 6727 _l 6728 _ 
               
               
                 d 6729 _d 6730  f 6731 _v 6732 _m 6733 _i 6734 _l 6735 _k 6736   
               
               
                   
               
               
                 (SEQ ID NO: 165) 
                   
               
               
                 h 6705 _n 6706 _y 6707 _f 6708 _i 6709 _t 6710 _e 6711 _t 6712 _n 6713 _t 6714 _a 6715 _a 6716 _f 6717 _k 6718 _a 6719 _v 6720 _c 6721 _s 6722 _v 6723 _ 
                   
               
               
                 i 6724  d 6725 _l 6726 _k 6727 _l 6728 _d 6729 _d 6730 _f 6731 _v 6732 _m 6733 _i 6734 _l 6735 _k 6736   
               
               
                   
               
               
                 (SEQ ID NO: 166) 
                   
               
               
                 h 6705 _n 6706 _y 6707 _f 6708 _i 6709 _t 6710 _e 6711 _t 6712 _n 6713 _t 6714 _a 6715 _a 6716 _f 6717 _k 6718 _a 6719 _v 6720 _c 6721 _s 6722 _v 6723 _ 
                   
               
               
                 i 6724  d 6725 _l 6726 _k 6727 _l 6728 _d 6729 _d 6730 _f 6731 _v 6732 _m 6733 _i 6734 _l 6735 _k 6736 _s 6737 _q 6738 _d 6739 _l 6740 _g 6741 _v 6742 _ 
               
               
                 v 6743 _s 6744  k 6745   
               
               
                   
               
               
                 (SEQ ID NO: 167) 
                   
               
               
                 h 6705 _n 6706 _y 6707 _f 6708 _i 6709 _t 6710 _e 6711 _t 6712 _n 6713 _t 6714 _a 6715 _a 6716 _f 6717 _k 6718 _a 6719 _v 6720 _c 6721 _s 6722 _v 6723 _ 
                   
               
               
                 i 6724  d 6725 _l 6726 _k 6727   
               
               
                   
               
               
                 (SEQ ID NO: 168) 
                   
               
               
                 s 6737 _q 6738 _d 6739 _l 6740 _g 6741 _v 6742 _v 6743 _s 6744 _k 6745 _v 6746 _v 6747 _k 6748 _v 6749 _p 6750 _i 6751 _d 6752 _l 6753 _t 6754  m 6755 _ 
                   
               
               
                 i 6756 _e 6757 _f 6758 _m 6759 _l 6760 _w 6761 _c 6762 _k 6763 _d 6764 _g 6765 _q 6766 _v 6767 _q 6768 _t 6769 _f 6770 _y 6771 _p 6772 _r 6773  l 6774 _ 
               
               
                 q 6775 _a 6776 _s 6777 _a 6778 _d 6779 _w 6780 _k 6781 _p 6782 _g 6783 _h 6784   
               
               
                   
               
               
                 (SEQ ID NO: 21) 
                   
               
               
                 k 6781 _p 6782 _g 6783 _h 6784 _a 6785 _m 6786 _p 6787 _s 6788 _l 6789 _f 6790 _k 6791   
                   
               
               
                   
               
               
                 (SEQ ID NO: 169) 
                   
               
               
                 k 6781 _p 6782 _g 6783 _h 6784 _a 6785 _m 6786 _p 6787 _s 6788 _l 6789 _f 6790 _k 6791 _v 6792 _q 6793 _n 6794 _v 6795 _n 6796 _l 6797 _e 6798 _r 6799   
                   
               
               
                 c 6800 _e 6801 _l 6802 _a 6803 _n 6804 _y 6805 _k 6806 _q 6807 _s 6808 _i 6809 _p 6810 _m 6811 _p 6812 _r 6813 _g 6814 _v 6815 _h 6816   
               
               
                   
               
               
                 (SEQ ID NO: 170) 
                   
               
               
                 k 6912 _n 6913 _v 6914 _t 6915 _g 6916 _s 6917 _n 6918 _e 6919 _s 6920 _k 6921 _a 6922 _l 6923 _f 6924 _f 6925 _t 6926 _y 6927 _l 6928 _c 6929 _n 6930 _ 
                   
               
               
                 l 6931  i 6932 _n 6933 _n 6934 _n 6935 _l 6936 _a 6937 _l 6938 _g 6939 _g 6940 _s 6941 _v 6942 _a 6943 _i 6944 _k 6945 _i 6946 _t 6947 _e 6948 _h 6949   
               
               
                   
               
               
                 (SEQ ID NO: 171) 
                   
               
               
                 h 7000 _a 7001 _n 7002 _y 7003 _i 7004 _f 7005 _w 7006 _r 7007 _n 7008 _s 7009 _t 7010 _p 7011 _m 7012 _n 7013 _l 7014 _s 7015 _t 7016 _y 7017 _s 7018 _ 
                   
               
               
                 l 7019  f 7020 _d 7021 _l 7022 _s 7023 _k 7024 _f 7025 _q 7026 _l 7027 _k 7028 _l 7029 _k 7030   
               
               
                   
               
               
                 (SEQ ID NO: 172) 
                   
               
               
                 h 7000 _a 7001 _n 7002 _y 7003 _i 7004 _f 7005 _w 7006 _r 7007 _n 7008 _s 7009 _t 7010 _p 7011 _m 7012 _n 7013 _l 7014 _s 7015 _t 7016 _y 7017 _s 7018 _ 
                   
               
               
                 l 7019  f 7020 _d 7021 _l 7022 _s 7023 _k 7024 _f 7025 _q 7026 _l 7027 _k 7028 _l 7029 _k 7030 _g 7031 _t 7032 _p 7033 _v 7034 _l 7035 _q 7036 _l 7037 _ 
               
               
                 k 7038   
               
               
                   
               
               
                 Replikin Count in AHX71944 is the number of Replikin sequences per 100 amino acid 
               
               
                 residues, which is 162/7078 and equals 2.3. 
               
               
                 Sequence History by Year for Replikin Sequences Identified in Accession No. 
               
               
                 AHX71944 
               
               
                   
               
               
                 (SEQ ID NO: 10) 
                   
               
               
                 KSVVRHLGVTK 
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 2094, YP 007188578 position 2094, AGV08378 position 2094, 
               
               
                 AGV08377 position 2094, AGV08583 position 2094, AGV08582 position 2094, 
               
               
                 AGV08407 position 2094, AGV08406 position 2094, AFY13306 position 2094, 
               
               
                 AFY13305 position 2094, AGG22541 position 2094, AGG22540 position 2094, 
               
               
                 AGH58716 position 2094, AGH58715 position 2094, AFV09327 position 2094, 
               
               
                 AFS88944 position 2094. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22564 position 2094, AHY22563 position 2094, AHY22554 position 2094, 
               
               
                 AHY22553 position 2094, AHY22544 position 2094, AHY22543 position 2094, 
               
               
                 AHY22534 position 2094, AHY22533 position 2094, AHY22524 position 2094, 
               
               
                 AHY22523 position 2094, AHE78107 position 2094, AHE78106 position 2094, 
               
               
                 AHE78096 position 2094, AHE78095 position 2094, AHX00730 position 2094, 
               
               
                 AHX00729 position 2094, AHX00720 position 2094, AHX00719 position 2094, 
               
               
                 AHX00710 position 2094, AHX00709 position 2094, K9N638 position 2094, 
               
               
                 K9N7C7 position 2094, AHN10811 position 2094, AHN10810 position 2094, 
               
               
                 AHI48798 position 1460, AHI48796 position 799, AHI48795 position 785, 
               
               
                 AHI48794 position 1460, AHI48792 position 1460, AHI48789 position 1415, 
               
               
                 AHI48787 position 764, AHI48786 position 133, AHI48784 position 1460, 
               
               
                 AHI48777 position 1451, AHI48776 position 1460, AHI48769 position 2094, 
               
               
                 AHI48768 position 2094, AHI48767 position 2094, AHI48766 position 2094, 
               
               
                 AHI48765 position 2094, AHI48764 position 2094, AHI48763 position 2094, 
               
               
                 AHI48762 position 2094, AHI48761 position 2094, AHI48760 position 2094, 
               
               
                 AHI48759 position 2094, AHI48758 position 2094, AHI48757 position 2094, 
               
               
                 AHI48756 position 2094, AHI48755 position 2094, AHI48754 position 2094, 
               
               
                 AHI48625 position 1361, AHI48624 position 1361, AHI48615 position 1475, 
               
               
                 AHI48614 position 1475, AHI48604 position 2094, AHI48603 position 2094, 
               
               
                 AHI48593 position 2094, AHI48592 position 2094, AHI48582 position 2094, 
               
               
                 AHI48581 position 2094, AHI48571 position 2094, AHI48570 position 2094, 
               
               
                 AHI48560 position 2094, AHI48559 position 2094, AHI48549 position 2094, 
               
               
                 AHI48548 position 2094, AHI48538 position 2094, AHI48537 position 2094, 
               
               
                 AHI48527 position 2094, AHI48526 position 2094, AHI48516 position 2094, 
               
               
                 AHI48515 position 2094, AHL18091 position 2094, AHL18089 position 2094, 
               
               
                 AHC74096 position 2094, AHC74086 position 2094, AGV08596 position 2094, 
               
               
                 AGV08595 position 2094, AGV08572 position 2094, AGV08571 position 2094, 
               
               
                 AGV08557 position 2094, AGV08556 position 2094, AGV08555 position 681, 
               
               
                 AGV08545 position 2094, AGV08544 position 2094, AGV08534 position 2094, 
               
               
                 AGV08533 position 2094, AGV08522 position 767, AGV08515 position 136, 
               
               
                 AGV08491 position 2094, AGV08490 position 2094, AGV08489 position 339, 
               
               
                 AGV08479 position 2094, AGV08478 position 2094, AGV08466 position 2094, 
               
               
                 AGV08465 position 2094, AGV08454 position 2094, AGV08453 position 2094, 
               
               
                 AGV08443 position 2094, AGV08442 position 2094, AGV08402 position 682, 
               
               
                 AGV08401 position 138, AGV08389 position 2094, AGV08388 position 2094, 
               
               
                 AGN72639 position 2094, AGN72638 position 2094, AGN70972 position 2094, 
               
               
                 AGN70971 position 2094, AGN70961 position 2094, AGN70960 position 2094, 
               
               
                 AGN70950 position 2094, AGN70949 position 2094, AGN70928 position 2094, 
               
               
                 AGN70927 position 2094. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 2094, AHY21467 position 2094, AHX71945 position 2094, 
               
               
                 AHX71944 position 2094. 
               
               
                   
               
               
                 (SEQ ID NO: 11) 
                   
               
               
                 KFYQHVINGCK 
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 2364, YP_007188578 position 2364, AGV08378 position 2364, 
               
               
                 AGV08377 position 2364, AGV08583 position 2364, AGV08582 position 2364, 
               
               
                 AGV08407 position 2364, AGV08406 position 2364, AFY13306 position 2364, 
               
               
                 AFY13305 position 2364, AGG22541 position 2364, AGG22540 position 2364, 
               
               
                 AGH58716 position 2364, AGH58715 position 2364, AFV09327 position 2364, 
               
               
                 AFS88944 position 2364. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22564 position 2364, AHY22563 position 2364, AHY22554 position 2364, 
               
               
                 AHY22553 position 2364, AHY22544 position 2364, AHY22543 position 2364, 
               
               
                 AHY22534 position 2364, AHY22533 position 2364, AHY22524 position 2364, 
               
               
                 AHY22523 position 2364, AHE78107 position 2364, AHE78106 position 2364, 
               
               
                 AHE78096 position 2364, AHE78095 position 2364, AHX00730 position 2364, 
               
               
                 AHX00729 position 2364, AHX00720 position 2364, AHX00719 position 2364, 
               
               
                 AHX00710 position 2364, AHX00709 position 2364, K9N638 position 2364, 
               
               
                 K9N7C7 position 2364, AHN10811 position 2364, AHN10810 position 2364, 
               
               
                 AHI48798 position 1730, AHI48796 position 1069, AHI48795 position 1055, 
               
               
                 AHI48794 position 1730, AHI48792 position 1730, AHI48789 position 1685, 
               
               
                 AHI48786 position 403, AHI48784 position 1730, AHI48776 position 1730, 
               
               
                 AHI48769 position 2364, AHI48768 position 2364, AHI48767 position 2364, 
               
               
                 AHI48766 position 2364, AHI48765 position 2364, AHI48764 position 2364, 
               
               
                 AHI48763 position 2364, AHI48762 position 2364, AHI48761 position 2364, 
               
               
                 AHI48760 position 2364, AHI48759 position 2364, AHI48758 position 2364, 
               
               
                 AHI48757 position 2364, AHI48756 position 2364, AHI48755 position 2364, 
               
               
                 AHI48754 position 2364, AHI48625 position 1631, AHI48624 position 1631, 
               
               
                 AHI48615 position 1745, AHI48614 position 1745, AHI48604 position 2364, 
               
               
                 AHI48603 position 2364, AHI48593 position 2364, AHI48592 position 2364, 
               
               
                 AHI48582 position 2364, AHI48581 position 2364, AHI48571 position 2364, 
               
               
                 AHI48570 position 2364, AHI48560 position 2364, AHI48559 position 2364, 
               
               
                 AHI48549 position 2364, AHI48548 position 2364, AHI48538 position 2364, 
               
               
                 AHI48537 position 2364, AHI48527 position 2364, AHI48526 position 2364, 
               
               
                 AHI48516 position 2364, AHI48515 position 2364, AHL18091 position 2364, 
               
               
                 AHL18089 position 2364, AHC74096 position 2364, AHC74086 position 2364, 
               
               
                 AHC74080 position 221, AHB33325 position 2364, AHB33324 position 2364, 
               
               
                 AGV08596 position 2364, AGV08595 position 2364, AGV08572 position 2364, 
               
               
                 AGV08571 position 2364, AGV08557 position 2364, AGV08556 position 2364, 
               
               
                 AGV08555 position 951, AGV08545 position 2364, AGV08544 position 2364, 
               
               
                 AGV08534 position 2364, AGV08533 position 2364, AGV08522 position 1037, 
               
               
                 AGV08515 position 406, AGV08491 position 2364, AGV08490 position 2364, 
               
               
                 AGV08489 position 609, AGV08479 position 2364, AGV08478 position 2364, 
               
               
                 AGV08466 position 2364, AGV08465 position 2364, AGV08454 position 2364, 
               
               
                 AGV08453 position 2364, AGV08443 position 2364, AGV08442 position 2364, 
               
               
                 AGV08402 position 952, AGV08401 position 408, AGV08389 position 2364, 
               
               
                 AGV08388 position 2364, AGN72639 position 2364, AGN72638 position 2364, 
               
               
                 AGN70972 position 2364, AGN70971 position 2364, AGN70961 position 2364, 
               
               
                 AGN70960 position 2364, AGN70950 position 2364, AGN70949 position 2364, 
               
               
                 AGN70928 position 2364, AGN70927 position 2364. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 2364, AHY21467 position 2364, AHX71945 position 2364, 
               
               
                 AHX71944 position 2364. 
               
               
                   
               
               
                 (SEQ ID NO: 6) 
                   
               
               
                 KHVEVFTDGK 
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP 007188577 position 3147, YP 007188578 position 3147, AGV08378 position 3147, 
               
               
                 AGV08377 position 3147, AGV08583 position 3147, AGV08582 position 3147, 
               
               
                 AGV08407 position 3147, AGV08406 position 3147, AFY13306 position 3147, 
               
               
                 AFY13305 position 3147, AGG22541 position 3147, AGG22540 position 3147, 
               
               
                 AGH58716 position 3147, AGH58715 position 3147, AFV09327 position 3147, 
               
               
                 AFS88944 position 3147. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22564 position 3147, AHY22563 position 3147, AHY22554 position 3147, 
               
               
                 AHY22553 position 3147, AHY22544 position 3147, AHY22543 position 3147, 
               
               
                 AHY22534 position 3147, AHY22533 position 3147, AHY22524 position 3147, 
               
               
                 AHY22523 position 3147, AHE78107 position 3147, AHE78106 position 3147, 
               
               
                 AHE78096 position 3147, AHE78095 position 3147, AHX00730 position 3147, 
               
               
                 AHX00729 position 3147, AHX00720 position 3147, AHX00719 position 3147, 
               
               
                 AHX00710 position 3147, AHX00709 position 3147, K9N638 position 3147, 
               
               
                 K9N7C7 position 3147, AHN10811 position 3147, AHN10810 position 3147, 
               
               
                 AHI48798 position 2513, AHI48796 position 1852, AHI48795 position 1838, 
               
               
                 AHI48794 position 2513, AHI48790 position 480, AHI48788 position 700, 
               
               
                 AHI48780 position 449, AHI48776 position 2513, AHI48769 position 3147, 
               
               
                 AHI48768 position 3147, AHI48767 position 3147, AHI48766 position 3147, 
               
               
                 AHI48765 position 3147, AHI48764 position 3147, AHI48763 position 3147, 
               
               
                 AHI48762 position 3147, AHI48761 position 3147, AHI48760 position 3147, 
               
               
                 AHI48759 position 3147, AHI48758 position 3147, AHI48757 position 3147, 
               
               
                 AHI48756 position 3147, AHI48755 position 3147, AHI48754 position 3147, 
               
               
                 AHI48747 position 337, AHI48746 position 337, AHI48625 position 2414, 
               
               
                 AHI48624 position 2414, AHI48615 position 2528, AHI48614 position 2528, 
               
               
                 AHI48604 position 3147, AHI48603 position 3147, AHI48593 position 3147, 
               
               
                 AHI48592 position 3147, AHI48582 position 3147, AHI48581 position 3147, 
               
               
                 AHI48571 position 3147, AHI48570 position 3147, AHI48560 position 3147, 
               
               
                 AHI48559 position 3147, AHI48549 position 3147, AHI48548 position 3147, 
               
               
                 AHI48538 position 3147, AHI48537 position 3147, AHI48527 position 3147, 
               
               
                 AHI48526 position 3147, AHI48516 position 3147, AHI48515 position 3147, 
               
               
                 AHL18091 position 3147, AHL18089 position 3147, AHC74096 position 3147, 
               
               
                 AHC74086 position 3147, AHB33325 position 3147, AHB33324 position 3147, 
               
               
                 AGV08596 position 3147, AGV08595 position 3147, AGV08572 position 3147, 
               
               
                 AGV08571 position 3147, AGV08557 position 3147, AGV08556 position 3147, 
               
               
                 AGV08555 position 1734, AGV08545 position 3147, AGV08544 position 3147, 
               
               
                 AGV08534 position 3147, AGV08533 position 3147, AGV08515 position 1189, 
               
               
                 AGV08491 position 3147, AGV08490 position 3147, AGV08479 position 3147, 
               
               
                 AGV08478 position 3147, AGV08466 position 3147, AGV08465 position 3147, 
               
               
                 AGV08454 position 3147, AGV08453 position 3147, AGV08443 position 3147, 
               
               
                 AGV08442 position 3147, AGV08401 position 1191, AGV08389 position 3147, 
               
               
                 AGV08388 position 3147, AGN72639 position 3147, AGN72638 position 3147, 
               
               
                 AGN70972 position 3147, AGN70971 position 3147, AGN70961 position 3147, 
               
               
                 AGN70960 position 3147, AGN70950 position 3147, AGN70949 position 3147, 
               
               
                 AGN70928 position 3147, AGN70927 position 3147. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 3147, AHY21467 position 3147, AHX71945 position 3147, 
               
               
                 AHX71944 position 3147. 
               
            
           
         
       
     
     Example 5 
     Analysis of Accession No. AFY13314 for Replikin Sequences Conserved in Human Host and Shared with Isolates in Camel Hosts 
       
     
       
         
           
               
               
            
               
                 PubMed Code: 
                   
               
               
                   
               
               
                 PubMed Code: 
               
               
                 AFY13314 
               
               
                   
               
               
                 Description: 
               
               
                 Direct Submission Direct Submission 
               
               
                   
               
               
                 Isolated: 
               
               
                 2012 in United Kingdom 
               
               
                   
               
               
                 Source: 
               
               
                 Betacoronavirus England 1 
               
               
                   
               
               
                 Strain: 
               
               
                 England 1 
               
               
                 (SEQ ID NO: 173) 
                   
               
               
                 m 1  a 2  s 3  p 4  a 5  a 6  p 7  r 8  a 9  v 10  s 11  l 42  a 13  d 14  n 15  n 16  d 17  i 18  t 19  n 20  t 21  n 22  l 23  s 24  r 25  g 26  r 27  g 28  r 29   
                   
               
               
                   
               
               
                 n 30  p 31  k 32  p 33  r 34  a 35  a 36  p 37  n 38  n 39  t 40  v 41  s 42  w 43  y 44  t 45  g 46  l 47  t 48  q 49  h 50  g 51  k 52  v 53  p 54  l 55  t 56   
               
               
                   
               
               
                 f 57  p 58  p 59  g 60  q 61  g 62  v 63  p 64  l 65  n 66  a 67  n 68  s 69  t 70  p 71  a 72  q 73  n 74  a 75  g 76  y 77  w 78  r 79  r 80  q 81  d 82  r 83   
               
               
                   
               
               
                 k 84  i 85  n 86  t 87  g 88  n 89  g 90  i 91  k 92  q 93  l 94  a 95  p 96  r 97  w 98  y 99  f 100  y 101  y 102  t 103  g 104  t 105  g 106  p 107  e 108   
               
               
                   
               
               
                 a 109  a 110  l 111  p 112  f 113  r 114  a 115  v 116  k 117  d 118  g 119  l 120  v 121  w 122  v 123  h 124  e 125  d 126  g 127  a 128  t 129   
               
               
                   
               
               
                 d 130  a 131  p 132  s 133  t 134  l 135  g 136  t 137  r 138  a 139  p 140  a 141  a 142  d 143  s 144  a 145  i 146  v 147  t 148  q 149  l 450  a 151  p 152   
               
               
                   
               
               
                 g 153  t 154  k 155  l 156  p 157  k 158  p 159  l 460  h 161  i 162  e 163  g 164  t 165  g 166  g 167  a 168  s 169  q 170  s 171  s 172  s 173  r 174  a 175   
               
               
                   
               
               
                 s 176  s 177  v 178  s 179  r 180  a 181  s 182  s 183  r 184  s 185  s 186  s 187  q 188  g 189  s 190  r 191  s 192  g 193  a 194  s 195  t 196  r 197  g 198   
               
               
                   
               
               
                 t 199  s 200  p 201  g 202  p 203  s 204  g 205  i 206  g 207  a 208  v 209  g 210  g 211  d 212  l 213  l 214  y 215  l 216  d 217  l 218  l 219  a 220  r 221   
               
               
                   
               
               
                 l 222  q 223  a 224  l 225  e 226  s 227  g 228  k 229  e 230  k 231  q 232  s 233  q 234  p 235  k 236  v 237  i 238  t 239  k 240  k 241  d 242  a 243  a 244   
               
               
                   
               
               
                 a 245  a 246  k 247  a 248  k 249  m 250  r 251  h 252  k 253  r 254  t 255  s 256  t 257  k 258  s 259  f 260  a 261  m 262  v 263  q 264  a 265  f 266  g 267   
               
               
                   
               
               
                 l 268  r 269  g 270  p 271  g 272  d 273  l 274  q 275  g 276  a 277  f 278  g 279  d 280  l 281  q 282  l 283  a 284  k 285  l 286  g 287  t 288  e 289  d 290   
               
               
                   
               
               
                 p 291  r 292  w 293  p 294  q 295  i 296  a 297  e 298  l 299  a 300  p 301  t 302  a 303  s 304  a 305  f 306  m 307  g 308  m 309  s 310  q 311  f 312  k 313   
               
               
                   
               
               
                 l 314  t 315  h 316  q 317  a 318  a 319  d 320  d 321  h 322  g 323  a 324  p 325  v 326  y 327  f 328  l 329  r 330  y 331  s 332  g 333  a 334  i 335  k 336   
               
               
                   
               
               
                 l 337  d 338  p 339  k 340  p 341  p 342  a 343  y 344  p 345  k 346  w 347  l 348  e 349  l 350  l 351  e 352  q 353  a 354  i 355  d 356  a 357  y 358  k 359   
               
               
                   
               
               
                 t 360  f 361  p 362  k 363  k 364  e 365  k 366  k 367  q 368  k 369  a 370  p 371  k 372  e 373  e 374  s 375  t 376  d 377  q 378  m 379  s 380  e 381  p 382   
               
               
                   
               
               
                 p 383  k 384  e 385  h 386  r 387  v 388  q 389  g 390  t 391  q 392  r 393  t 394  r 395  t 396  r 397  p 398  s 399  v 400  q 401  p 402  g 403  p 404  m 405   
               
               
                 i 406  d 407  v 408  a 409  t 410  d 411   
               
               
                   
               
               
                   
               
               
                 Amino-terminal 
               
               
                 (SEQ ID NO: 174) 
                   
               
               
                 h 50 _g 51 _k 52 _v 53 _p 54 _l 55 _t 56 _f 57 _p 58 _p 59 _g 60 _q 61 _g 62 _v 63 _p 64 _l 65 _n 66 _a 67 _n 68 _s 69 _t 70 _p 71 _a 72 _q 73 _n 74 _a 75 _g 76 _y 77   
                   
               
               
                 w 78 _r 79 _r 80 _q 81 _d 82 _r 83 _k 84 _i 85 _n 86 _t 87 _g 88 _n 89 _g 90 _i 91 _k 92   
               
               
                   
               
               
                 (SEQ ID NO: 175) 
                   
               
               
                 k 84 _i 85 _n 86 _t 87 _g 88 _n 89 _g 90 _i 91 _k 92  q 93  l 94 _a 95 _p 96 _r 97 _w 98 _y 99 _f 100 _y 101 _y 102 _t 103 _g 104 _t 105 _g 106 _p 107 _e 108 _a 109   
                   
               
               
                 a 110 _l 111 _p 112 _f 113 _r 114 _a 115 _v 116  k 117 _d 118 _g 119 _i 120 _v 121 _w 122 _v 123 _h 124   
               
               
                   
               
               
                 Mid-molecule 
               
               
                 (SEQ ID NO: 176) 
                   
               
               
                 k 229 _v 230 _k 231 _q 232 _s 233 _q 234 _p 235 _k 236 _v 237 _i 238 _t 239 _k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251   
                   
               
               
                 h 252   
               
               
                   
               
               
                 (SEQ ID NO: 177) 
                   
               
               
                 k 231 _q 232 _s 233 _q 234 _p 235 _k 236 _v 237 _i 238 _t 239 _k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 196) 
                   
               
               
                 k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252    
                   
               
               
                   
               
               
                 (SEQ ID NO: 24) 
                   
               
               
                 k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 22) 
                   
               
               
                 k 247 _n 248 _k 249 _m 250 _r 251 _h 252 _k 253   
                   
               
               
                   
               
               
                 (SEQ ID NO: 25) 
                   
               
               
                 k 249 _m 250 _r 251 _h 252 _k 253 _r 254 _t 255 _s 256 _t 257 _k 258   
                   
               
               
                   
               
               
                 Carboxy-terminal 
               
               
                 (SEQ ID NO: 178) 
                   
               
               
                 h 316 _q 317 _n 318 _n 319 _d 320 _d 321 _h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338   
                   
               
               
                 p 339 _k 340 _n 341 _p 342 _n 343 _y 344 _n 345 _k 346   
               
               
                   
               
               
                 (SEQ ID NO: 179) 
                   
               
               
                 h 316 _q 317 _n 318 _n 319 _d 320 _d 321 _h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338   
                   
               
               
                 p 339 _k 340 _n 341 _p 342 _n 343 _y 344 _n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361   
               
               
                 p 362 _k 363 _k 364 _e 365 _k 366   
               
               
                   
               
               
                 (SEQ ID NO: 180) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346   
               
               
                   
               
               
                 (SEQ ID NO: 181) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350  l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366   
               
               
                   
               
               
                   
               
               
                 (SEQ ID NO: 182) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350  l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                   
               
               
                 (SEQ ID NO: 183) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350  l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                 g 368 _k 369   
               
               
                   
               
               
                 (SEQ ID NO: 184) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350  l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                 g 368 _k 369  a 370 _p 371 _k 372   
               
               
                   
               
               
                 (SEQ ID NO: 185) 
                   
               
               
                 k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344 _n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358   
                   
               
               
                 k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381   
               
               
                 p 382 _p 383 _k 384 _e 385 _h 386   
               
               
                   
               
               
                 (SEQ ID NO: 186) 
                   
               
               
                 k 340 _n 341 _p 342 _n 343 _y 344 _n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362   
                   
               
               
                 k 363 _k 364 _e 365 _k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381 _p 382 _p 383 _k 384 _e 385   
               
               
                 h 386   
               
               
                   
               
               
                 (SEQ ID NO: 187) 
                   
               
               
                 k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381   
                   
               
               
                 p 382 _p 383 _k 384 _e 385 _h 386   
               
               
                   
               
               
                 (SEQ ID NO: 188) 
                   
               
               
                 k 363 _k 364 _e 365 _k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381 _p 382 _p 383 _k 384 _e 385   
                   
               
               
                 h 386   
               
               
                   
               
               
                 (SEQ ID NO: 189) 
                   
               
               
                 k 364 _e 365 _k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381 _p 382 _p 383 _k 384 _e 385 _h 386   
                   
               
               
                   
               
               
                 (SEQ ID NO: 190) 
                   
               
               
                 k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381 _p 382 _p 383 _k 384 _e 385 _h 386   
                   
               
               
                   
               
               
                 Replikin Count in AFY13314 is the number of Replikin sequences per 100 amino acid 
               
               
                 residues, which is 21/411 and equals 5.1. 
               
               
                   
               
               
                 Sequence History by Year for Replikin Sequences Identified in Accession No. 
               
               
                 AFY13314 
               
               
                   
               
               
                 (SEQ ID NO: 12) 
                   
               
               
                 KQVHQVQLTDK  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 3438, YP_007188578 position 3438, AGV08378 position 3438, 
               
               
                   
               
               
                 AGV08377 position 3438, AGV08583 position 3438, AGV08582 position 3438, 
               
               
                   
               
               
                 AGV08407 position 3438, AGV08406 position 3438, AFY13306 position 3438, 
               
               
                   
               
               
                 AFY13305 position 3438, AGG22541 position 3438, AGG22540 position 3438, 
               
               
                   
               
               
                 AGH58716 position 3438, AGH58715 position 3438, AFV09327 position 3438, 
               
               
                   
               
               
                 AFS 88944 position 3438. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22564 position 3438, AHY22563 position 3438, AHY22554 position 3438, 
               
               
                   
               
               
                 AHY22553 position 3438, AHY22544 position 3438, AHY22543 position 3438, 
               
               
                   
               
               
                 AHY22534 position 3438, AHY22533 position 3438, AHY22524 position 3438, 
               
               
                   
               
               
                 AHY22523 position 3438, AHE78107 position 3438, AHE78106 position 3438, 
               
               
                   
               
               
                 AHE78096 position 3438, AHE78095 position 3438, AHX00730 position 3438, 
               
               
                   
               
               
                 AHX00729 position 3438, AHX00720 position 3438, AHX00719 position 3438, 
               
               
                   
               
               
                 AHX00710 position 3438, AHX00709 position 3438, K9N638 position 3438, K9N7C7 
               
               
                   
               
               
                 position 3438, AHN10811 position 3438, AHN10810 position 3438, AHI48798 position 
               
               
                   
               
               
                 2804, AHI48795 position 2129, AHI48794 position 2804, AHI48776 position 2804, 
               
               
                   
               
               
                 AHI48769 position 3438, AHI48768 position 3438, AHI48767 position 3438, AHI48766 
               
               
                   
               
               
                 position 3438, AHI48765 position 3438, AHI48764 position 3438, AHI48763 position 
               
               
                   
               
               
                 3438, AHI48762 position 3438, AHI48761 position 3438, AHI48760 position 3438, 
               
               
                   
               
               
                 AHI48759 position 3438, AHI48758 position 3438, AHI48757 position 3438, AHI48756 
               
               
                   
               
               
                 position 3438, AHI48755 position 3438, AHI48754 position 3438, AHI48749 position 117, 
               
               
                   
               
               
                 AHI48748 position 117, AHI48747 position 628, AHI48746 position 628, AHI48745 
               
               
                   
               
               
                 position 219, AHI48744 position 219, AHI48625 position 2705, AHI48624 position 2705, 
               
               
                   
               
               
                 AHI48615 position 2819, AHI48614 position 2819, AHI48604 position 3438, AHI48603 
               
               
                   
               
               
                 position 3438, AHI48593 position 3438, AHI48592 position 3438, AHI48582 position 
               
               
                   
               
               
                 3438, AHI48581 position 3438, AHI48571 position 3438, AHI48570 position 3438, 
               
               
                   
               
               
                 AHI48560 position 3438, AHI48559 position 3438, AHI48549 position 3438, AHI48548 
               
               
                   
               
               
                 position 3438, AHI48538 position 3438, AHI48537 position 3438, AHI48527 position 
               
               
                   
               
               
                 3438, AHI48526 position 3438, AHI48516 position 3438, AHI48515 position 3438, 
               
               
                   
               
               
                 AHL18091 position 3438, AHL18089 position 3438, AHC74096 position 3438, 
               
               
                   
               
               
                 AHC74086 position 3438, AHC74081 position 173, AHB33325 position 3438, AHB33324 
               
               
                   
               
               
                 position 3438, AGV08596 position 3438, AGV08595 position 3438, AGV08594 position 
               
               
                   
               
               
                 116, AGV08572 position 3438, AGV08571 position 3438, AGV08557 position 3438, 
               
               
                   
               
               
                 AGV08556 position 3438, AGV08555 position 2025, AGV08545 position 3438, 
               
               
                   
               
               
                 AGV08544 position 3438, AGV08534 position 3438, AGV08533 position 3438, 
               
               
                   
               
               
                 AGV08515 position 1480, AGV08491 position 3438, AGV08490 position 3438, 
               
               
                   
               
               
                 AGV08479 position 3438, AGV08478 position 3438, AGV08466 position 3438, 
               
               
                   
               
               
                 AGV08465 position 3438, AGV08454 position 3438, AGV08453 position 3438, 
               
               
                   
               
               
                 AGV08443 position 3438, AGV08442 position 3438, AGV08401 position 1482, 
               
               
                   
               
               
                 AGV08389 position 3438, AGV08388 position 3438, AGN72639 position 3438, 
               
               
                   
               
               
                 AGN72638 position 3438, AGN70972 position 3438, AGN70971 position 3438, 
               
               
                   
               
               
                 AGN70961 position 3438, AGN70960 position 3438, AGN70950 position 3438, 
               
               
                   
               
               
                 AGN70949 position 3438, AGN70928 position 3438, AGN70927 position 3438. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 3438, AHY21467 position 3438, AHX71945 position 3438, 
               
               
                   
               
               
                 AHX71944 position 3438. 
               
               
                   
               
               
                 (SEQ ID NO: 13) 
                   
               
               
                 KGDSCSSNCKH  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 390, YP_007188578 position 390, AGV08378 position 390, 
               
               
                   
               
               
                 AGV08377 position 390, AGV08583 position 390, AGV08582 position 390, AGV08407 
               
               
                   
               
               
                 position 390, AGV08406 position 390, AFY13306 position 390, AFY13305 position 390, 
               
               
                   
               
               
                 AGG22541 position 390, AGG22540 position 390, AGH58716 position 390, AGH58715 
               
               
                   
               
               
                 position 390, AFV09327 position 390, AFS88944 position 390. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22564 position 390, AHY22563 position 390, AHY22554 position 390, AHY22553 
               
               
                   
               
               
                 position 390, AHY22544 position 390, AHY22543 position 390, AHY22534 position 390, 
               
               
                   
               
               
                 AHY22533 position 390, AHY22524 position 390, AHY22523 position 390, AHE78107 
               
               
                   
               
               
                 position 390, AHE78106 position 390, AHE78096 position 390, AHE78095 position 390, 
               
               
                   
               
               
                 AHX00730 position 390, AHX00729 position 390, AHX00720 position 390, AHX00719 
               
               
                   
               
               
                 position 390, AHX00710 position 390, AHX00709 position 390, K9N638 position 390, 
               
               
                   
               
               
                 K9N7C7 position 390, AHN10811 position 390, AHN10810 position 390, AHI48797 
               
               
                   
               
               
                 position 390, AHI48793 position 390, AHI48791 position 390, AHI48785 position 105, 
               
               
                   
               
               
                 AHI48783 position 390, AHI48782 position 390, AHI48778 position 390, AHI48769 
               
               
                   
               
               
                 position 390, AHI48768 position 390, AHI48767 position 390, AHI48766 position 390, 
               
               
                   
               
               
                 AHI48765 position 390, AHI48764 position 390, AHI48763 position 390, AHI48762 
               
               
                   
               
               
                 position 390, AHI48761 position 390, AHI48760 position 390, AHI48759 position 390, 
               
               
                   
               
               
                 AHI48758 position 390, AHI48757 position 390, AHI48756 position 390, AHI48755 
               
               
                   
               
               
                 position 390, AHI48754 position 390, AHI48604 position 390, AHI48603 position 390, 
               
               
                   
               
               
                 AHI48593 position 390, AHI48592 position 390, AHI48582 position 390, AHI48581 
               
               
                   
               
               
                 position 390, AHI48571 position 390, AHI48570 position 390, AHI48560 position 390, 
               
               
                   
               
               
                 AHI48559 position 390, AHI48549 position 390, AHI48548 position 390, AHI48538 
               
               
                   
               
               
                 position 390, AHI48537 position 390, AHI48527 position 390, AHI48526 position 390, 
               
               
                   
               
               
                 AHI48516 position 390, AHI48515 position 390, AHL18091 position 390, AHL18089 
               
               
                   
               
               
                 position 390, AHC74096 position 390, AHC74086 position 390, AHB33325 position 390, 
               
               
                   
               
               
                 AHB33324 position 390, AGV08596 position 390, AGV08595 position 390, AGV08593 
               
               
                   
               
               
                 position 161, AGV08572 position 390, AGV08571 position 390, AGV08567 position 390, 
               
               
                   
               
               
                 AGV08557 position 390, AGV08556 position 390, AGV08545 position 390, AGV08544 
               
               
                   
               
               
                 position 390, AGV08534 position 390, AGV08533 position 390, AGV08491 position 390, 
               
               
                   
               
               
                 AGV08490 position 390, AGV08479 position 390, AGV08478 position 390, AGV08466 
               
               
                   
               
               
                 position 390, AGV08465 position 390, AGV08464 position 390, AGV08454 position 390, 
               
               
                   
               
               
                 AGV08453 position 390, AGV08443 position 390, AGV08442 position 390, AGV08403 
               
               
                   
               
               
                 position 390, AGV08389 position 390, AGV08388 position 390, AGN72639 position 390, 
               
               
                   
               
               
                 AGN72638 position 390, AGN70972 position 390, AGN70971 position 390, AGN70961 
               
               
                   
               
               
                 position 390, AGN70960 position 390, AGN70950 position 390, AGN70949 position 390, 
               
               
                   
               
               
                 AGN70928 position 390, AGN70927 position 390. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 390, AHY21467 position 390, AHX71945 position 390, AHX71944 
               
               
                   
               
               
                 position 390. 
               
            
           
         
       
     
     Example 6 
     Analysis of Accession No. YP_007188586 for Replikin Sequences Conserved in Human Host and Shared with Isolates in Camel Hosts 
       
     
       
         
           
               
               
            
               
                 PubMed Code: 
                   
               
               
                 YP_007188586 
               
               
                   
               
               
                 Description: 
               
               
                 Direct Submission Direct Submission Direct Submission 
               
               
                   
               
               
                 Isolated: 
               
               
                 2012 in United Kingdom 
               
               
                   
               
               
                 Source: 
               
               
                 Middle East respiratory syndrome coronavirus (MERS-CoV) 
               
               
                   
               
               
                 Strain: 
               
               
                 England 1 
               
               
                 (SEQ ID NO: 191) 
                   
               
               
                 m 1  a 2  s 3  p 4  a 5  a 6  p 7  r 8  a 9  v 10  s 11  f 12  a 13  d 14  n 15  n 16  d 17  i 18  t 19  n 20  t 21  n 22  l 23  s 24  r 25  g 26  r 27  g 28  r 29  n 30  p 31   
                   
               
               
                   
               
               
                 k 32  p 33  r 34  a 35  a 36  p 37  n 38  n 39  t 40  v 41  s 42  w 43  y 44  t 45  g 46  l 47  t 48  q 49  h 50  g 51  k 52  v 53  p 54  l 55  t 56  f 57  p 58  p 59  g 60   
               
               
                   
               
               
                 q 61  g 62  v 63  p 64  l 65  n 66  a 67  n 68  s 69  t 70  p 71  a 72  q 73  n 74  a 75  g 76  y 77  w 78  r 79  r 80  q 81  d 82  r 83  k 84  i 85  n 86  t 87  g 88  n 89   
               
               
                   
               
               
                 g 90  i 91  k 92  q 93  l 94  a 95  p 96  r 97  w 98  y 99  f 100  y 101  y 102  t 103  g 104  t 105  g 106  p 107  e 108  a 109  a 110  l 111  p 112  f 113  r 114  a 115   
               
               
                   
               
               
                 v 116  k 117  d 118  g 119  i 120  v 121  w 122  v 123  h 124  e 125  d 126  g 127  a 128  t 129  d 130  a 131  p 132  s 133  t 134  f 135  g 136  t 137  r 138  n 139   
               
               
                   
               
               
                 p 140  n 141  n 142  d 143  s 144  a 145  i 146  v 147  t 148  q 149  f 150  a 151  p 152  g 153  t 154  k 155  l 156  p 157  k 158  n 159  f 160  h 161  i 162  e 163   
               
               
                   
               
               
                 g 164  t 165  g 166  g 167  n 168  s 169  q 170  s 171  s 172  s 173  r 174  a 175  s 176  s 177  v 178  s 179  r 180  n 181  s 182  s 183  r 184  s 185  s 186  s 187   
               
               
                   
               
               
                 q 188  g 189  s 190  r 191  s 192  g 193  n 194  s 195  t 196  r 197  g 198  t 199  s 200  p 201  g 202  p 203  s 204  g 205  i 206  g 207  a 208  v 209  g 210  g 211   
               
               
                   
               
               
                 d 212  l 213  l 214  y 215  l 216  d 217  l 218  l 219  n 220  r 221  l 222  q 223  a 224  l 225  e 226  s 227  g 228  k 229  v 230  k 231  q 232  s 233  q 234  p 235   
               
               
                   
               
               
                 k 236  v 237  i 238  t 239  k 240  k 241  d 242  a 243  a 244  a 245  a 246  k 247  n 248  k 249  m 250  r 251  h 252  k 253  r 254  t 255  s 256  t 257  k 258  s 259   
               
               
                   
               
               
                 f 260  n 261  m 262  v 263  q 264  a 265  f 266  g 267  l 268  r 269  g 270  p 271  g 272  d 273  l 274  q 275  g 276  n 277  f 278  g 279  d 280  l 281  q 282  l 283   
               
               
                   
               
               
                 n 284  k 285  l 286  g 287  t 288  e 289  d 290  p 291  r 292  w 293  p 294  q 295  i 296  a 297  e 298  l 299  a 300  p 301  t 302  a 303  s 304  a 305  f 306  m 307   
               
               
                   
               
               
                 g 308  m 309  s 310  q 311  f 312  k 313  l 314  t 315  h 316  q 317  n 318  n 319  d 320  d 321  h 322  g 323  n 324  p 325  v 326  y 327  f 328  l 329  r 330  y 331   
               
               
                   
               
               
                 s 332  g 333  a 334  i 335  k 336  l 337  d 338  p 339  k 340  p 341  p 342  n 343  y 344  p 345  k 346  w 347  l 348  e 349  l 350  l 351  e 352  q 353  n 354  i 355   
               
               
                   
               
               
                 d 356  a 357  y 358  k 359  t 360  f 361  p 362  k 363  k 364  e 365  k 366  k 367  q 368  k 369  a 370  p 371  k 372  e 373  e 374  s 375  t 376  d 377  q 378  m 379   
               
               
                   
               
               
                 s 380  e 381  p 382  p 383  k 384  e 385  h 386  r 387  v 388  q 389  g 390  t 391  q 392  r 393  t 394  r 395  t 396  r 397  p 398  s 399  V 400  q 401  p 402  g 40   
               
               
                   
               
               
                 p 404  m 405  i 406  d 407  v 408  n 409  t 410  d 411   
               
               
                   
               
               
                   
               
               
                 Amino-terminal 
               
               
                 (SEQ ID NO: 191) 
                   
               
               
                 h 50 _g 51 _k 52 _v 53 _p 54 _l 55 _t 56 _f 57 _p 58 _p 59 _g 60 _g 61 _g 62 _v 63 _p 64 _l 65 _n 66 _a 67 _n 68 _s 69 _t 70 _p 71 _a 72 _q 73 _n 74 _a 75 _g 76 _y 77   
                   
               
               
                 w 78 _r 79 _r 80 _q 81 _d 82 _r 83 _k 84 _i 85 _n 86 _t 87 _g 88 _n 89 _g 90 _i 91 _k 92   
               
               
                   
               
               
                 (SEQ ID NO: 192) 
                   
               
               
                 k 84 _i 85 _n 86 _t 87 _g 88 _n 89 _g 90 _i 91 _k 92 _q 93 _l 94 _a 95 _p 96 _r 97 _w 98 _y 99 _f 100 _y 101 _y 102 _t 103 _g 104 _t 105 _g 106 _p 107 _e 108 _a 109   
                   
               
               
                 a 110 _l 111 _p 112 _f 113 _r 114 _a 115 _v 116 _k 117 _d 118 _g 119 _i 120 _v 121 _w 122 _v 123 _h 124   
               
               
                   
               
               
                 Mid-molecule 
               
               
                 (SEQ ID NO: 194) 
                   
               
               
                 k 229 _v 230 _k 231 _q 232 _s 233 _q 234 _p 235 _k 236 _v 237 _i 238 _t 239 _k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251   
                   
               
               
                 h 252   
               
               
                   
               
               
                 (SEQ ID NO: 195) 
                   
               
               
                 k 231 _q 232 _s 233 _q 234 _p 235 _k 236 _v 237 _i 238 _t 239 _k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 196) 
                   
               
               
                 k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 24) 
                   
               
               
                 k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 22) 
                   
               
               
                 k 247 _n 248 _k 249 _m 250 _r 251 _h 252 _k 253   
                   
               
               
                   
               
               
                 (SEQ ID NO: 25) 
                   
               
               
                 k 249 _m 250 _r 251 _h 252 _k 253 _r 254 _t 255 _s 256 _t 257 _k 258   
                   
               
               
                   
               
               
                 Carboxy-terminal 
               
               
                 (SEQ ID NO: 197) 
                   
               
               
                 h 316 _q 317 _n 318 _n 319 _d 320 _d 321 _h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338   
                   
               
               
                 p 339 _k 340 _n 341 _p 342 _n 343 _y 344 _n 345 _k 346   
               
               
                   
               
               
                 (SEQ ID NO: 198) 
                   
               
               
                 h 316 _q 317 _n 318 _n 319 _d 320 _d 321 _h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338   
                   
               
               
                 p 339 _k 340 _n 341 _p 342 _n 343 _y 344 _n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361   
               
               
                 p 362 _k 363 _k 364 _e 365 _k 366   
               
               
                   
               
               
                 (SEQ ID NO: 199) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346   
               
               
                   
               
               
                 (SEQ ID NO: 200) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 a 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366   
               
               
                   
               
               
                 (SEQ ID NO: 201) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 a 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                   
               
               
                 (SEQ ID NO: 202) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 a 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                 q 368 _k 369   
               
               
                   
               
               
                 (SEQ ID NO: 203) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                 q 368 _k 369 _a 370 _p 371 _k 372   
               
               
                   
               
               
                 (SEQ ID NO: 204) 
                   
               
               
                 k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344 _n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358   
                   
               
               
                 k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381   
               
               
                 p 382 _p 383 _k 384 _e 385 _h 386   
               
               
                   
               
               
                 (SEQ ID NO: 205) 
                   
               
               
                 k 340 _n 341 _p 342 _n 343 _y 344 _n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362   
                   
               
               
                 k 363 _k 364 _e 365 _k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381 _p 382 _p 383 _k 384 _e 385   
               
               
                 h 386   
               
               
                   
               
               
                 (SEQ ID NO: 206) 
                   
               
               
                 k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381   
                   
               
               
                 p 382 _p 383 _k 384 _e 385 _h 386   
               
               
                   
               
               
                 (SEQ ID NO: 207) 
                   
               
               
                 k 363 _k 364 _e 365 _k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381 _p 382 _p 383 _k 384 _e 385   
                   
               
               
                 h 386   
               
               
                   
               
               
                 (SEQ ID NO: 208) 
                   
               
               
                 k 364 _e 365 _k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381 _p 382 _p 383 _k 384 _e 385 _h 386   
                   
               
               
                   
               
               
                 (SEQ ID NO: 209) 
                   
               
               
                 k 366 _k 367 _q 368 _k 369 _a 370 _p 371 _k 372 _e 373 _e 374 _s 375 _t 376 _d 377 _q 378 _m 379 _s 380 _e 381 _p 382 _p 383 _k 384 _e 385 _h 386   
                   
               
               
                   
               
               
                 Replikin Count in AFY13314 is the number of Replikin sequences per 100 amino acid 
               
               
                 residues, which is 21/411 and equals 5.1. 
               
               
                   
               
               
                 Sequence History by Year for Replikin Sequences Identified in Accession No. 
               
               
                 YP_007188586 
               
               
                   
               
               
                 (SEQ ID NO: 14) 
                   
               
               
                 HARLKGGLILK  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 1762, YP_007188578 position 1762, AGV08378 position 1762, 
               
               
                   
               
               
                 AGV08377 position 1762, AGV08583 position 1762, AGV08582 position 1762, 
               
               
                   
               
               
                 AGV08407 position 1762, AGV08406 position 1762, AFY13306 position 1762, 
               
               
                   
               
               
                 AFY13305 position 1762, AGG22541 position 1762, AGG22540 position 1762, 
               
               
                   
               
               
                 AGH58716 position 1762, AGH58715 position 1762, AFV09327 position 1762, 
               
               
                   
               
               
                 AFS88944 position 1762. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22564 position 1762, AHY22563 position 1762, AHY22554 position 1762, 
               
               
                   
               
               
                 AHY22553 position 1762, AHY22544 position 1762, AHY22543 position 1762, 
               
               
                   
               
               
                 AHY22534 position 1762, AHY22533 position 1762, AHY22524 position 1762, 
               
               
                   
               
               
                 AHY22523 position 1762, AHE78107 position 1762, AHE78106 position 1762, 
               
               
                   
               
               
                 AHE78096 position 1762, AHE78095 position 1762, AHX00730 position 1762, 
               
               
                   
               
               
                 AHX00729 position 1762, AHX00720 position 1762, AHX00719 position 1762, 
               
               
                   
               
               
                 AHX00710 position 1762, AHX00709 position 1762, K9N638 position 1762, K9N7C7 
               
               
                   
               
               
                 position 1762, AHN10811 position 1762, AHN10810 position 1762, AHI48796 position 
               
               
                   
               
               
                 467, AHI48795 position 453, AHI48794 position 1128, AHI48792 position 1128, 
               
               
                   
               
               
                 AHI48789 position 1083, AHI48787 position 432, AHI48784 position 1128, AHI48779 
               
               
                   
               
               
                 position 433, AHI48777 position 1119, AHI48776 position 1128, AHI48769 position 1762, 
               
               
                   
               
               
                 AHI48768 position 1762, AHI48767 position 1762, AHI48766 position 1762, AHI48765 
               
               
                   
               
               
                 position 1762, AHI48764 position 1762, AHI48761 position 1762, AHI48760 position 
               
               
                   
               
               
                 1762, AHI48759 position 1762, AHI48758 position 1762, AHI48757 position 1762, 
               
               
                   
               
               
                 AHI48756 position 1762, AHI48755 position 1762, AHI48754 position 1762, AHI48625 
               
               
                   
               
               
                 position 1029, AHI48624 position 1029, AHI48615 position 1143, AHI48614 position 
               
               
                   
               
               
                 1143, AHI48604 position 1762, AHI48603 position 1762, AHI48593 position 1762, 
               
               
                   
               
               
                 AHI48592 position 1762, AHI48582 position 1762, AHI48581 position 1762, AHI48571 
               
               
                   
               
               
                 position 1762, AHI48570 position 1762, AHI48549 position 1762, AHI48548 position 
               
               
                   
               
               
                 1762, AHI48527 position 1762, AHI48526 position 1762, AHI48516 position 1762, 
               
               
                   
               
               
                 AHI48515 position 1762, AHL18091 position 1762, AHL18089 position 1762, AHC74096 
               
               
                   
               
               
                 position 1762, AHC74086 position 1762, AHB33325 position 1762, AHB33324 position 
               
               
                   
               
               
                 1762, AGV08596 position 1762, AGV08595 position 1762, AGV08572 position 1762, 
               
               
                   
               
               
                 AGV08571 position 1762, AGV08557 position 1762, AGV08556 position 1762, 
               
               
                   
               
               
                 AGV08555 position 349, AGV08545 position 1762, AGV08544 position 1762, 
               
               
                   
               
               
                 AGV08534 position 1762, AGV08533 position 1762, AGV08522 position 435, 
               
               
                   
               
               
                 AGV08491 position 1762, AGV08490 position 1762, AGV08489 position 7, AGV08479 
               
               
                   
               
               
                 position 1762, AGV08478 position 1762, AGV08466 position 1762, AGV08465 position 
               
               
                   
               
               
                 1762, AGV08454 position 1762, AGV08453 position 1762, AGV08443 position 1762, 
               
               
                   
               
               
                 AGV08442 position 1762, AGV08402 position 350, AGN72639 position 1762, 
               
               
                   
               
               
                 AGN72638 position 1762, AGN70972 position 1762, AGN70971 position 1762, 
               
               
                   
               
               
                 AGN70961 position 1762, AGN70960 position 1762, AGN70950 position 1762, 
               
               
                   
               
               
                 AGN70949 position 1762, AGN70928 position 1762, AGN70927 position 1762. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 1762, AHY21467 position 1762, AHX71945 position 1762, 
               
               
                   
               
               
                 AHX71944 position 1762. 
               
               
                   
               
               
                 (SEQ ID NO: 15) 
                   
               
               
                 KAMLLKKEPLLYVPIRLAGH  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 60, YP_007188578 position 60, AGV08378 position 60, 
               
               
                   
               
               
                 AGV08377 position 60, AGV08583 position 60, AGV08582 position 60, AGV08407 
               
               
                   
               
               
                 position 60, AGV08406 position 60, AFY13306 position 60, AFY13305 position 60, 
               
               
                   
               
               
                 AGG22541 position 60, AGG22540 position 60, AGH58716 position 60, AGH58715 
               
               
                   
               
               
                 position 60, AFV09327 position 60, AFS88944 position 60. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22564 position 60, AHY22563 position 60, AHY22554 position 60, AHY22553 
               
               
                   
               
               
                 position 60, AHY22544 position 60, AHY22543 position 60, AHY22534 position 60, 
               
               
                   
               
               
                 AHY22533 position 60, AHY22524 position 60, AHY22523 position 60, AHE78107 
               
               
                   
               
               
                 position 60, AHE78106 position 60, AHE78096 position 60, AHE78095 position 60, 
               
               
                   
               
               
                 AHX00730 position 60, AHX00729 position 60, AHX00720 position 60, AHX00719 
               
               
                   
               
               
                 position 60, AHX00710 position 60, AHX00709 position 60, K9N638 position 60, 
               
               
                   
               
               
                 K9N7C7 position 60, AHN10811 position 60, AHN10810 position 60, AHI48797 position 
               
               
                   
               
               
                 60, AHI48793 position 60, AHI48791 position 60, AHI48783 position 60, AHI48782 
               
               
                   
               
               
                 position 60, AHI48778 position 60, AHI48769 position 60, AHI48768 position 60, 
               
               
                   
               
               
                 AHI48767 position 60, AHI48766 position 60, AHI48765 position 60, AHI48764 position 
               
               
                   
               
               
                 60, AHI48763 position 60, AHI48762 position 60, AHI48761 position 60, AHI48760 
               
               
                   
               
               
                 position 60, AHI48759 position 60, AHI48758 position 60, AHI48757 position 60, 
               
               
                   
               
               
                 AHI48756 position 60, AHI48755 position 60, AHI48754 position 60, AHI48604 position 
               
               
                   
               
               
                 60, AHI48603 position 60, AHI48593 position 60, AHI48592 position 60, AHI48582 
               
               
                   
               
               
                 position 60, AHI48581 position 60, AHI48571 position 60, AHI48570 position 60, 
               
               
                   
               
               
                 AHI48560 position 60, AHI48559 position 60, AHI48549 position 60, AHI48548 position 
               
               
                   
               
               
                 60, AHI48538 position 60, AHI48537 position 60, AHI48527 position 60, AHI48526 
               
               
                   
               
               
                 position 60, AHI48516 position 60, AHI48515 position 60, AHL18091 position 60, 
               
               
                   
               
               
                 AHL18089 position 60, AHC74096 position 60, AHC74086 position 60, AHB33325 
               
               
                   
               
               
                 position 60, AHB33324 position 60, AGV08596 position 60, AGV08595 position 60, 
               
               
                   
               
               
                 AGV08572 position 60, AGV08571 position 60, AGV08567 position 60, AGV08557 
               
               
                   
               
               
                 position 60, AGV08556 position 60, AGV08545 position 60, AGV08544 position 60, 
               
               
                   
               
               
                 AGV08534 position 60, AGV08533 position 60, AGV08491 position 60, AGV08490 
               
               
                   
               
               
                 position 60, AGV08479 position 60, AGV08478 position 60, AGV08466 position 60, 
               
               
                   
               
               
                 AGV08465 position 60, AGV08464 position 60, AGV08454 position 60, AGV08453 
               
               
                   
               
               
                 position 60, AGV08443 position 60, AGV08442 position 60, AGV08403 position 60, 
               
               
                   
               
               
                 AGV08389 position 60, AGV08388 position 60, AGN72639 position 60, AGN72638 
               
               
                   
               
               
                 position 60, AGN70972 position 60, AGN70971 position 60, AGN70961 position 60, 
               
               
                   
               
               
                 AGN70960 position 60, AGN70950 position 60, AGN70949 position 60, AGN70928 
               
               
                   
               
               
                 position 60, AGN70927 position 60 . 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 60, AHY21467 position 60, AHX71945 position 60, AHX71944 
               
               
                 position 60 . 
               
               
                   
               
               
                 (SEQ ID NO: 2) 
                   
               
               
                 KSAGHPFNK  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 4878, AGV08377 position 4878, AGV08582 position 4878, 
               
               
                   
               
               
                 AGV08406 position 4878, AFY13306 position 4878, AGG22540 position 4878, 
               
               
                   
               
               
                 AGH58716 position 4878, AFS88944 position 4878. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22563 position 4878, AHY22553 position 4878, AHY22543 position 4878, 
               
               
                   
               
               
                 AHY22533 position 4878, AHY22523 position 4878, AHE78106 position 4878, 
               
               
                   
               
               
                 AHE78095 position 4878, AHX00730 position 4878, AHX00720 position 4878, 
               
               
                   
               
               
                 AHX00710 position 4878, K9N7C7 position 4878, AHN10810 position 4878, AHI48776 
               
               
                   
               
               
                 position 4244, AHI48774 position 200, AHI48772 position 200, AHI48768 position 4878, 
               
               
                   
               
               
                 AHI48766 position 4878, AHI48764 position 4878, AHI48762 position 4878, AHI48760 
               
               
                   
               
               
                 position 4878, AHI48758 position 4878, AHI48756 position 4878, AHI48754 position 
               
               
                   
               
               
                 4878, AHI48748 position 1557, AHI48744 position 1659, AHI48624 position 4145, 
               
               
                   
               
               
                 AHI48614 position 4259, AHI48603 position 4878, AHI48592 position 4878, AHI48581 
               
               
                   
               
               
                 position 4878, AHI48570 position 4878, AHI48559 position 4878, AHI48548 position 
               
               
                   
               
               
                 4878, AHI48537 position 4878, AHI48526 position 4878, AHI48515 position 4878, 
               
               
                   
               
               
                 AHL18091 position 4878, AHC74097 position 485, AHC74087 position 485, AHB33324 
               
               
                   
               
               
                 position 4878, AGV08595 position 4878, AGV08571 position 4878, AGV08556 position 
               
               
                   
               
               
                 4878, AGV08544 position 4878, AGV08533 position 4878, AGV08523 position 257, 
               
               
                   
               
               
                 AGV08504 position 89, AGV08490 position 4878, AGV08478 position 4878, AGV08465 
               
               
                   
               
               
                 position 4878, AGV08453 position 4878, AGV08442 position 4878, AGV08437 position 
               
               
                   
               
               
                 83, AGV08435 position 182, AGV08388 position 4878, AGN72639 position 4878, 
               
               
                   
               
               
                 AGN70971 position 4878, AGN70960 position 4878, AGN70949 position 4878, 
               
               
                   
               
               
                 AGN70927 position 4878 . 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 4878 , AHX71944 position 4878 . 
               
               
                   
               
               
                 (SEQ ID NO: 16) 
                   
               
               
                 KHLVPLMHK  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 6055, AGV08377 position 6055, AGV08582 position 6055, 
               
               
                   
               
               
                 AGV08406 position 6055, AFY13306 position 6055, AGG22540 position 6055, 
               
               
                   
               
               
                 AGH58716 position 6055, AFS88944 position 6055. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22563 position 6055, AHY22553 position 6055, AHY22543 position 6055, 
               
               
                   
               
               
                 AHY22533 position 6055, AHY22523 position 6055, AHE78106 position 6055, 
               
               
                   
               
               
                 AHE78095 position 6055, AHX00730 position 6055, AHX00720 position 6055, 
               
               
                   
               
               
                 AHX00710 position 6055, K9N7C7 position 6055, AHN10810 position 6055, AHI48776 
               
               
                   
               
               
                 position 5421, AHI48770 position 703, AHI48768 position 6055, AHI48766 position 6055, 
               
               
                   
               
               
                 AHI48764 position 6055, AHI48762 position 6055, AHI48760 position 6055, AHI48756 
               
               
                   
               
               
                 position 6055, AHI48754 position 6055, AHI48624 position 5322, AHI48614 position 
               
               
                   
               
               
                 5436, AHI48603 position 6055, AHI48592 position 6055, AHI48581 position 6055, 
               
               
                   
               
               
                 AHI48570 position 6055, AHI48559 position 6055, AHI48548 position 6055, AHI48537 
               
               
                   
               
               
                 position 6055, AHI48526 position 6055, AHI48515 position 6055, AHC74097 position 
               
               
                   
               
               
                 1662, AHC74087 position 1662, AHB33324 position 6055, AGV08597 position 521, 
               
               
                   
               
               
                 AGV08595 position 6055, AGV08571 position 6055, AGV08556 position 6055, 
               
               
                   
               
               
                 AGV08544 position 6055, AGV08533 position 6055, AGV08523 position 1434, 
               
               
                   
               
               
                 AGV08504 position 1266, AGV08490 position 6055, AGV08478 position 6055, 
               
               
                   
               
               
                 AGV08465 position 6055, AGV08453 position 6055, AGV08442 position 6055, 
               
               
                   
               
               
                 AGV08437 position 1260, AGV08388 position 6055, AGN72639 position 6055, 
               
               
                   
               
               
                 AGN70971 position 6055, AGN70960 position 6055, AGN70949 position 6055, 
               
               
                   
               
               
                 AGN70927 position 6055. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 6055 , AHX71944 position 6055 . 
               
               
                   
               
               
                 (SEQ ID NO: 8) 
                   
               
               
                 KKHVEVFTDGK  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 3146, YP_007188578 position 3146, AGV08378 position 3146, 
               
               
                   
               
               
                 AGV08377 position 3146, AGV08583 position 3146, AGV08582 position 3146, 
               
               
                   
               
               
                 AGV08407 position 3146, AGV08406 position 3146, AFY13306 position 3146, 
               
               
                   
               
               
                 AFY13305 position 3146, AGG22541 position 3146, AGG22540 position 3146, 
               
               
                   
               
               
                 AGH58716 position 3146, AGH58715 position 3146, AFV09327 position 3146, 
               
               
                   
               
               
                 AFS88944 position 3146. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22564 position 3146, AHY22563 position 3146, AHY22554 position 3146, 
               
               
                   
               
               
                 AHY22553 position 3146, AHY22544 position 3146, AHY22543 position 3146, 
               
               
                   
               
               
                 AHY22534 position 3146, AHY22533 position 3146, AHY22524 position 3146, 
               
               
                   
               
               
                 AHY22523 position 3146, AHE78107 position 3146, AHE78106 position 3146, 
               
               
                   
               
               
                 AHE78096 position 3146, AHE78095 position 3146, AHX00730 position 3146, 
               
               
                   
               
               
                 AHX00729 position 3146, AHX00720 position 3146, AHX00719 position 3146, 
               
               
                   
               
               
                 AHX00710 position 3146, AHX00709 position 3146, K9N638 position 3146 , K9N7C7 
               
               
                   
               
               
                 position 3146, AHN10811 position 3146, AHN10810 position 3146, AHI48798 position 
               
               
                   
               
               
                 2512, AHI48796 position 1851, AHI48795 position 1837, AHI48794 position 2512, 
               
               
                   
               
               
                 AHI48790 position 479, AHI48788 position 699, AHI48780 position 448, AHI48776 
               
               
                   
               
               
                 position 2512, AHI48769 position 3146, AHI48768 position 3146, AHI48767 position 
               
               
                   
               
               
                 3146, AHI48766 position 3146, AHI48765 position 3146, AHI48764 position 3146, 
               
               
                   
               
               
                 AHI48763 position 3146, AHI48762 position 3146, AHI48761 position 3146, AHI48760 
               
               
                   
               
               
                 position 3146, AHI48759 position 3146, AHI48758 position 3146, AHI48757 position 
               
               
                   
               
               
                 3146, AHI48756 position 3146, AHI48755 position 3146, AHI48754 position 3146, 
               
               
                   
               
               
                 AHI48747 position 336, AHI48746 position 336, AHI48625 position 2413, AHI48624 
               
               
                   
               
               
                 position 2413, AHI48615 position 2527, AHI48614 position 2527, AHI48604 position 
               
               
                   
               
               
                 3146, AHI48603 position 3146, AHI48593 position 3146, AHI48592 position 3146, 
               
               
                   
               
               
                 AHI48582 position 3146, AHI48581 position 3146, AHI48571 position 3146, AHI48570 
               
               
                   
               
               
                 position 3146, AHI48560 position 3146, AHI48559 position 3146, AHI48549 position 
               
               
                   
               
               
                 3146, AHI48548 position 3146, AHI48538 position 3146, AHI48537 position 3146, 
               
               
                   
               
               
                 AHI48527 position 3146, AHI48526 position 3146, AHI48516 position 3146, AHI48515 
               
               
                   
               
               
                 position 3146, AHL18091 position 3146, AHL18089 position 3146, AHC74096 position 
               
               
                   
               
               
                 3146, AHC74086 position 3146, AHB33325 position 3146, AHB33324 position 3146, 
               
               
                   
               
               
                 AGV08596 position 3146, AGV08595 position 3146, AGV08572 position 3146, 
               
               
                   
               
               
                 AGV08571 position 3146, AGV08557 position 3146, AGV08556 position 3146, 
               
               
                   
               
               
                 AGV08555 position 1733, AGV08545 position 3146, AGV08544 position 3146, 
               
               
                   
               
               
                 AGV08534 position 3146, AGV08533 position 3146, AGV08515 position 1188, 
               
               
                   
               
               
                 AGV08491 position 3146, AGV08490 position 3146, AGV08479 position 3146, 
               
               
                   
               
               
                 AGV08478 position 3146, AGV08466 position 3146, AGV08465 position 3146, 
               
               
                   
               
               
                 AGV08454 position 3146, AGV08453 position 3146, AGV08443 position 3146, 
               
               
                   
               
               
                 AGV08442 position 3146, AGV08401 position 1190, AGV08389 position 3146, 
               
               
                   
               
               
                 AGV08388 position 3146, AGN72639 position 3146, AGN72638 position 3146, 
               
               
                   
               
               
                 AGN70972 position 3146, AGN70971 position 3146, AGN70961 position 3146, 
               
               
                   
               
               
                 AGN70960 position 3146, AGN70950 position 3146, AGN70949 position 3146, 
               
               
                   
               
               
                 AGN70928 position 3146, AGN70927 position 3146. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 3146, AHY21467 position 3146, AHX71945 position 3146, 
               
               
                   
               
               
                 AHX71944 position 3146. 
               
               
                   
               
               
                 (SEQ ID NO: 17) 
                   
               
               
                 KYYAFLNKH  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 5122, AGV08377 position 5122, AGV08582 position 5122, 
               
               
                   
               
               
                 AGV08406 position 5122, AFY13306 position 5122, AGG22540 position 5122, 
               
               
                   
               
               
                 AGH58716 position 5122, AFS88944 position 5122. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22563 position 5122, AHY22553 position 5122, AHY22543 position 5122, 
               
               
                   
               
               
                 AHY22533 position 5122, AHY22523 position 5122, AHE78106 position 5122, 
               
               
                   
               
               
                 AHE78095 position 5122, AHX00730 position 5122, AHX00720 position 5122, 
               
               
                   
               
               
                 AHX00710 position 5122, K9N7C7 position 5122, AHN10810 position 5122, AHI48776 
               
               
                   
               
               
                 position 4488, AHI48774 position 444, AHI48773 position 143, AHI48772 position 444, 
               
               
                   
               
               
                 AHI48768 position 5122, AHI48766 position 5122, AHI48764 position 5122, AHI48762 
               
               
                   
               
               
                 position 5122, AHI48760 position 5122, AHI48758 position 5122, AHI48756 position 
               
               
                   
               
               
                 5122, AHI48754 position 5122, AHI48748 position 1801, AHI48744 position 1903, 
               
               
                   
               
               
                 AHI48624 position 4389, AHI48614 position 4503, AHI48603 position 5122, AHI48592 
               
               
                   
               
               
                 position 5122, AHI48581 position 5122, AHI48570 position 5122, AHI48559 position 
               
               
                   
               
               
                 5122, AHI48548 position 5122, AHI48537 position 5122, AHI48526 position 5122, 
               
               
                   
               
               
                 AHI48515 position 5122, AHL18091 position 5122, AHC74097 position 729, AHC74087 
               
               
                   
               
               
                 position 729 , AHB33324 position 5122, AGV08595 position 5122, AGV08571 position 
               
               
                   
               
               
                 5122, AGV08556 position 5122, AGV08544 position 5122, AGV08533 position 5122, 
               
               
                   
               
               
                 AGV08523 position 501, AGV08504 position 333, AGV08490 position 5122, AGV08478 
               
               
                   
               
               
                 position 5122, AGV08465 position 5122, AGV08453 position 5122, AGV08442 position 
               
               
                   
               
               
                 5122, AGV08437 position 327, AGV08435 position 426, AGV08388 position 5122, 
               
               
                   
               
               
                 AGN72639 position 5122, AGN70971 position 5122, AGN70960 position 5122, 
               
               
                   
               
               
                 AGN70949 position 5122, AGN70927 position 5122. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 5122, AHX71944 position 5122. 
               
               
                   
               
               
                 (SEQ ID NO: 18) 
                   
               
               
                 KAAVHKWK  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 1568, YP 007188578 position 1568, AGV08378 position 1568, 
               
               
                   
               
               
                 AGV08377 position 1568, AGV08583 position 1568, AGV08582 position 1568, 
               
               
                   
               
               
                 AGV08407 position 1568, AGV08406 position 1568, AFY13306 position 1568, 
               
               
                   
               
               
                 AFY13305 position 1568, AGG22541 position 1568, AGG22540 position 1568. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22564 position 1568, AHY22563 position 1568, AHY22554 position 1568, 
               
               
                   
               
               
                 AHY22553 position 1568, AHY22544 position 1568, AHY22543 position 1568, 
               
               
                   
               
               
                 AHY22534 position 1568, AHY22533 position 1568, AHY22524 position 1568, 
               
               
                   
               
               
                 AHY22523 position 1568, AHX00730 position 1568, AHX00729 position 1568, 
               
               
                   
               
               
                 AHX00720 position 1568, AHX00719 position 1568, AHX00710 position 1568, 
               
               
                   
               
               
                 AHX00709 position 1568, K9N638 position 1568, K9N7C7 position 1568, AHI48798 
               
               
                   
               
               
                 position 934, AHI48796 position 273, AHI48795 position 259, AHI48794 position 934, 
               
               
                   
               
               
                 AHI48792 position 934, AHI48791 position 1568, AHI48789 position 889, AHI48787 
               
               
                   
               
               
                 position 238, AHI48779 position 239, AHI48777 position 925, AHI48776 position 934, 
               
               
                   
               
               
                 AHI48769 position 1568, AHI48768 position 1568, AHI48767 position 1568, AHI48766 
               
               
                   
               
               
                 position 1568, AHI48765 position 1568, AHI48764 position 1568, AHI48763 position 
               
               
                   
               
               
                 1568, AHI48762 position 1568, AHI48761 position 1568, AHI48760 position 1568 
               
               
                   
               
               
                 AHI48759 position 1568, AHI48758 position 1568, AHI48757 position 1568, AHI48756 
               
               
                   
               
               
                 position 1568, AHI48755 position 1568, AHI48754 position 1568, AHI48625 position 835, 
               
               
                   
               
               
                 AHI48624 position 835, AHI48615 position 949, AHI48614 position 949, AHI48604 
               
               
                   
               
               
                 position 1568, AHI48603 position 1568, AHI48571 position 1568, AHI48570 position 
               
               
                   
               
               
                 1568, AHI48560 position 1568, AHI48559 position 1568, AHI48538 position 1568, 
               
               
                   
               
               
                 AHI48537 position 1568, AHI48527 position 1568, AHI48526 position 1568, AHI48516 
               
               
                   
               
               
                 position 1568, AHI48515 position 1568, AHC74096 position 1568, AHC74086 position 
               
               
                   
               
               
                 1568, AHB33325 position 1568, AHB33324 position 1568, AGV08596 position 1568, 
               
               
                   
               
               
                 AGV08595 position 1568, AGV08572 position 1568, AGV08571 position 1568, 
               
               
                   
               
               
                 AGV08557 position 1568, AGV08556 position 1568, AGV08555 position 155, 
               
               
                   
               
               
                 AGV08545 position 1568, AGV08544 position 1568, AGV08534 position 1568, 
               
               
                   
               
               
                 AGV08533 position 1568, AGV08522 position 241, AGV08491 position 1568, 
               
               
                   
               
               
                 AGV08490 position 1568, AGV08479 position 1568, AGV08478 position 1568, 
               
               
                   
               
               
                 AGV08466 position 1568, AGV08465 position 1568, AGV08454 position 1568, 
               
               
                   
               
               
                 AGV08453 position 1568, AGV08443 position 1568, AGV08442 position 1568, 
               
               
                   
               
               
                 AGV08436 position 525, AGV08402 position 156, AGV08389 position 1568, AGV08388 
               
               
                   
               
               
                 position 1568, AGN72639 position 1568, AGN72638 position 1568, AGN70972 position 
               
               
                   
               
               
                 1568, AGN70971 position 1568, AGN70961 position 1568, AGN70960 position 1568, 
               
               
                   
               
               
                 AGN70950 position 1568, AGN70949 position 1568, AGN70928 position 1568, 
               
               
                   
               
               
                 AGN70927 position 1568. 
               
               
                   
               
               
                 2014 
               
               
                 AHX71945 position 1568, AHX71944 position 1568. 
               
               
                   
               
               
                 (SEQ ID NO: 19) 
                   
               
               
                 KLNPSEDFIKH  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 1402, YP_007188578 position 1402, AGV08378 position 1402, 
               
               
                   
               
               
                 AGV08377 position 1402, AGV08583 position 1402, AGV08582 position 1402, 
               
               
                   
               
               
                 AGV08407 position 1402, AGV08406 position 1402, AFY13306 position 1402, 
               
               
                   
               
               
                 AFY13305 position 1402, AGG22541 position 1402, AGG22540 position 1402, 
               
               
                   
               
               
                 AGH58716 position 1402, AGH58715 position 1402, AFV09327 position 1402, 
               
               
                   
               
               
                 AFS88944 position 1402. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22564 position 1402, AHY22563 position 1402, AHY22554 position 1402, 
               
               
                   
               
               
                 AHY22553 position 1402, AHY22544 position 1402, AHY22543 position 1402, 
               
               
                   
               
               
                 AHY22534 position 1402, AHY22533 position 1402, AHY22524 position 1402, 
               
               
                   
               
               
                 AHY22523 position 1402, AHE78107 position 1402, AHE78106 position 1402, 
               
               
                   
               
               
                 AHE78096 position 1402, AHE78095 position 1402, AHX00730 position 1402, 
               
               
                   
               
               
                 AHX00729 position 1402, AHX00720 position 1402, AHX00719 position 1402, 
               
               
                   
               
               
                 AHX00710 position 1402, AHX00709 position 1402, K9N638 position 1402, K9N7C7 
               
               
                   
               
               
                 position 1402, AHN10811 position 1402, AHN10810 position 1402, AHI48798 position 
               
               
                   
               
               
                 768, AHI48796 position 107, AHI48795 position 93, AHI48794 position 768, AHI48792 
               
               
                   
               
               
                 position 768, AHI48791 position 1402, AHI48789 position 723, AHI48787 position 72, 
               
               
                   
               
               
                 AHI48784 position 768, AHI48779 position 73, AHI48777 position 759, AHI48776 
               
               
                   
               
               
                 position 768, AHI48769 position 1402, AHI48768 position 1402, AHI48767 position 1402, 
               
               
                   
               
               
                 AHI48766 position 1402, AHI48765 position 1402, AHI48764 position 1402, AHI48763 
               
               
                   
               
               
                 position 1402, AHI48762 position 1402, AHI48761 position 1402, AHI48760 position 
               
               
                   
               
               
                 1402, AHI48759 position 1402, AHI48758 position 1402, AHI48757 position 1402, 
               
               
                   
               
               
                 AHI48756 position 1402, AHI48755 position 1402, AHI48754 position 1402, AHI48625 
               
               
                   
               
               
                 position 669, AHI48624 position 669, AHI48615 position 783, AHI48614 position 783, 
               
               
                   
               
               
                 AHI48604 position 1402, AHI48603 position 1402, AHI48593 position 1402, AHI48592 
               
               
                   
               
               
                 position 1402, AHI48582 position 1402, AHI48581 position 1402, AHI48571 position 
               
               
                   
               
               
                 1402, AHI48570 position 1402, AHI48560 position 1402, AHI48559 position 1402, 
               
               
                   
               
               
                 AHI48549 position 1402, AHI48548 position 1402, AHI48538 position 1402, AHI48537 
               
               
                   
               
               
                 position 1402 , AHI48527 position 1402, AHI48526 position 1402, AHI48516 position 
               
               
                   
               
               
                 1402, AHI48515 position 1402, AHL18091 position 1402, AHL18089 position 1402, 
               
               
                   
               
               
                 AHC74096 position 1402, AHC74086 position 1402, AHB33325 position 1402, 
               
               
                   
               
               
                 AHB33324 position 1402, AGV08596 position 1402, AGV08595 position 1402, 
               
               
                   
               
               
                 AGV08572 position 1402, AGV08571 position 1402, AGV08557 position 1402, 
               
               
                   
               
               
                 AGV08556 position 1402, AGV08545 position 1402, AGV08544 position 1402, 
               
               
                   
               
               
                 AGV08534 position 1402, AGV08533 position 1402, AGV08522 position 75, AGV08491 
               
               
                   
               
               
                 position 1402, AGV08490 position 1402, AGV08479 position 1402, AGV08478 position 
               
               
                   
               
               
                 1402, AGV08466 position 1402, AGV08465 position 1402, AGV08454 position 1402, 
               
               
                   
               
               
                 AGV08453 position 1402, AGV08443 position 1402, AGV08442 position 1402, 
               
               
                   
               
               
                 AGV08436 position 359, AGV08389 position 1402, AGV08388 position 1402, 
               
               
                   
               
               
                 AGN72639 position 1402, AGN72638 position 1402, AGN70972 position 1402, 
               
               
                   
               
               
                 AGN70971 position 1402, AGN70961 position 1402, AGN70960 position 1402, 
               
               
                   
               
               
                 AGN70950 position 1402, AGN70949 position 1402, AGN70928 position 1402, 
               
               
                   
               
               
                 AGN70927 position 1402. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 1402, AHY21467 position 1402, AHX71945 position 1402, 
               
               
                   
               
               
                 AHX71944 position 1402. 
               
               
                   
               
               
                 (SEQ ID NO: 20) 
                   
               
               
                 KFCDHMVK  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 4569, AGV08377 position 4569, AGV08582 position 4569, 
               
               
                   
               
               
                 AGV08406 position 4569, AFY13306 position 4569, AGG22540 position 4569, 
               
               
                   
               
               
                 AGH58716 position 4569, AFS88944 position 4569. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22563 position 4569, AHY22553 position 4569, AHY22543 position 4569, 
               
               
                   
               
               
                 AHY22533 position 4569, AHY22523 position 4569, AHE78106 position 4569, 
               
               
                   
               
               
                 AHE78095 position 4569, AHX00730 position 4569, AHX00720 position 4569, 
               
               
                   
               
               
                 AHX00710 position 4569, K9N7C7 position 4569, AHN10810 position 4569, AHI48776 
               
               
                   
               
               
                 position 3935, AHI48775 position 518, AHI48768 position 4569, AHI48766 position 4569, 
               
               
                   
               
               
                 AHI48764 position 4569, AHI48762 position 4569, AHI48760 position 4569, AHI48758 
               
               
                   
               
               
                 position 4569, AHI48756 position 4569, AHI48754 position 4569, AHI48752 position 510, 
               
               
                   
               
               
                 AHI48750 position 606, AHI48748 position 1248, AHI48744 position 1350, AHI48740 
               
               
                   
               
               
                 position 520, AHI48624 position 3836, AHI48614 position 3950, AHI48603 position 4569, 
               
               
                   
               
               
                 AHI48592 position 4569, AHI48581 position 4569, AHI48570 position 4569, AHI48559 
               
               
                   
               
               
                 position 4569, AHI48548 position 4569, AHI48537 position 4569, AHI48526 position 
               
               
                   
               
               
                 4569, AHI48515 position 4569, AHL18091 position 4569, AHC74097 position 176, 
               
               
                   
               
               
                 AHC74087 position 176, AHB33324 position 4569, AGV08595 position 4569, AGV08571 
               
               
                   
               
               
                 position 4569, AGV08556 position 4569, AGV08544 position 4569, AGV08533 position 
               
               
                   
               
               
                 4569, AGV08490 position 4569, AGV08478 position 4569, AGV08465 position 4569, 
               
               
                   
               
               
                 AGV08453 position 4569, AGV08442 position 4569, AGV08388 position 4569, 
               
               
                   
               
               
                 AGN72639 position 4569, AGN70971 position 4569, AGN70960 position 4569, 
               
               
                   
               
               
                 AGN70949 position 4569, AGN70927 position 4569. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 4569 , AHX71944 position 4569 . 
               
               
                   
               
               
                 (SEQ ID NO: 21) 
                   
               
               
                 KPGHAMPSLFK  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188577 position 6781, AGV08377 position 6781, AGV08582 position 6781, 
               
               
                   
               
               
                 AGV08406 position 6781, AFY13306 position 6781, AGG22540 position 6781, 
               
               
                   
               
               
                 AGH58716 position 6781, AFS88944 position 6781. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22563 position 6781, AHY22553 position 6781, AHY22543 position 6781, 
               
               
                   
               
               
                 AHY22533 position 6781, AHY22523 position 6781, AHE78106 position 6781, 
               
               
                   
               
               
                 AHE78095 position 6781, AHX00730 position 6781, AHX00720 position 6781, 
               
               
                   
               
               
                 AHX00710 position 6781, K9N7C7 position 6781, AHI48776 position 6147, AHI48738 
               
               
                   
               
               
                 position 240, AHI48736 position 657, AHI48734 position 125, AHI48732 position 125, 
               
               
                   
               
               
                 AHI48730 position 125, AHI48728 position 180, AHI48726 position 154, AHI48724 
               
               
                   
               
               
                 position 119, AHI48722 position 125, AHI48720 position 124, AHI48710 position 125, 
               
               
                   
               
               
                 AHI48701 position 131, AHI48691 position 415, AHI48681 position 125, AHI48671 
               
               
                   
               
               
                 position 125, AHI48661 position 520, AHI48624 position 6048, AHI48614 position 6162, 
               
               
                   
               
               
                 AHI48603 position 6781, AHI48592 position 6781, AHI48581 position 6781, AHI48570 
               
               
                   
               
               
                 position 6781, AHI48559 position 6781, AHI48548 position 6781, AHI48537 position 
               
               
                   
               
               
                 6781, AHI48526 position 6781, AHI48515 position 6781, AHL31379 position 83, 
               
               
                   
               
               
                 AHL31377 position 83, AHL31375 position 83, AHL31373 position 83, AHL31371 
               
               
                   
               
               
                 position 83, AHL31369 position 83, AHL18091 position 6781, AHC74097 position 2388, 
               
               
                   
               
               
                 AHC74087 position 2388, AHB33324 position 6781, AGV08571 position 6781, 
               
               
                   
               
               
                 AGV08568 position 125, AGV08556 position 6781, AGV08544 position 6781, 
               
               
                   
               
               
                 AGV08533 position 6781, AGV08523 position 2160, AGV08519 position 67, AGV08504 
               
               
                   
               
               
                 position 1992, AGV08490 position 6781, AGV08478 position 6781, AGV08476 position 
               
               
                   
               
               
                 105, AGV08465 position 6781, AGV08453 position 6781, AGV08442 position 6781, 
               
               
                   
               
               
                 AGV08437 position 1986, AGV08425 position 125, AGV08399 position 125, AGV08388 
               
               
                   
               
               
                 position 6781, AGN72639 position 6781, AGN70971 position 6781, AGN70960 position 
               
               
                   
               
               
                 6781, AGN70949 position 6781, AGN70927 position 6781.. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21468 position 6781, AHX71944 position 6781. 
               
            
           
         
       
     
     Example 7 
     Analysis of Accession No. AHY21476 for Replikin Sequences Conserved in Human Host and Shared with Isolates in Camel Hosts 
       
     
       
         
           
               
               
            
               
                 PubMed Code: 
                   
               
               
                 AHY21476 
               
               
                   
               
               
                 Description: 
               
               
                 Full Genome Sequence of Human Betacoronavirus 2c Jordan-N3/2012 after Serial 
               
               
                 Passage in Mammalian Cells Direct Submission 
               
               
                   
               
               
                 Isolated: 
               
               
                 2014 
               
               
                   
               
               
                 Source: 
               
               
                 Human betacoronavirus 2c Jordan-N3/2012 
               
               
                   
               
               
                 Strain: 
               
               
                 Jordan-N3/2012 
               
               
                 (SEQ ID NO: 210) 
                   
               
               
                 m 1  a 2  s 3  p 4  a 5  a 6  p 7  r 8  a 9  v 10  s 11  f 12  a 13  y 14  n 15  n 16  d 17  i 18  t 19  n 20  t 21  n 22  l 23  s 24  r 25  g 26  r 27  g 28  r 29  n 30  p 31   
                   
               
               
                   
               
               
                 k 32  p 33  r 34  a 35  a 36  p 37  n 38  n 39  t 40  v 41  s 42  w 43  y 44  t 45  g 46  l 47  t 48  q 49  h 50  g 51  k 52  v 53  p 54  l 55  t 56  f 57  p 58  p 59  g 60   
               
               
                   
               
               
                 q 61  g 62  v 63  p 64  l 65  n 66  a 67  n 68  s 69  t 70  p 71  a 72  q 73  n 74  a 75  g 76  y 77  w 78  r 79  r 80  q 81  d 82  r 83  k 84  i 85  n 86  t 87  g 88  n 89   
               
               
                   
               
               
                 g 90  i 91  k 92  q 93  l 94  a 95  p 96  r 97  w 98  y 99  f 100  y 101  y 102  t 103  g 104  t 105  g 106  p 107  e 108  a 109  a 110  l 111  p 112  f 113  r 114  a 115   
               
               
                   
               
               
                 v 116  k 117  d 118  g 119  i 120  v 121  w 122  v 123  h 124  e 125  d 126  g 127  a 128  t 129  d 130  a 131  p 132  s 133  t 134  f 135  g 136  t 137  r 138  n 139   
               
               
                   
               
               
                 p 140  n 141  n 142  d 143  s 144  a 145  i 146  v 147  t 148  q 149  f 150  a 151  p 152  g 153  t 154  k 155  l 156  p 157  k 158  n 159  f 160  h 161  i 162  e 163   
               
               
                   
               
               
                 g 164  t 165  g 166  g 167  n 168  s 169  q 170  s 171  s 172  s 173  r 174  a 175  s 176  s 177  v 178  s 179  r 180  n 181  s 182  s 183  r 184  s 185   
               
               
                   
               
               
                 s 186  s 187  q 188  g 189  s 190  r 191  s 192  g 193  n 194  s 195  t 196  r 197  g 198  t 199  s 200  p 201  g 202  p 203  s 204  g 205  i 206  g 207  a 208  v 209   
               
               
                   
               
               
                 g 210  g 211  d 212  l 213  l 214  y 215  l 216  d 217  l 218  l 219  n 220  r 221  l 222  q 223  a 224  l 225  e 226  s 227  g 228  k 229  v 230  k 231  q 232  s 233   
               
               
                   
               
               
                 q 234  p 235  k 236  v 237  i 238  t 239  k 240  k 241  d 242  a 243  a 244  a 245  a 246  k 247  n 248  k 249  m 250  r 251  h 252  k 253  r 254  t 255  s 256  t 257   
               
               
                   
               
               
                 k 258  s 259  f 260  n 261  m 262  v 263  q 264  a 265  f 266  g 267  l 268  r 269  g 270  p 271  g 272  d 273  l 274  q 275  g 276  n 277  f 278  g 279  d 280  l 281   
               
               
                   
               
               
                 q 282  l 283  n 284  k 285  l 286  g 287  t 288  e 289  d 290  p 291  r 292  w 293  p 294  q 295  i 296  a 297  e 298  l 299  a 300  p 301  t 302  a 303  s 304  a 305   
               
               
                   
               
               
                 f 306  m 307  g 308  m 309  s 310  q 311  f 312  k 313  l 314  t 315  h 316  q 317  n 318  n 319  d 320  d 321  h 322  g 323  n 324  p 325  v 326  y 327  f 328  l 329   
               
               
                   
               
               
                 r 330  y 331  s 332  g 333  a 334  i 335  k 336  l 337  d 338  p 339  k 340  p 341  p 342  n 343  y 344  p 345  k 346  w 347  l 348  e 349  l 350  l 351  e 352  q 353   
               
               
                   
               
               
                 n 354  i 355  d 356  a 357  y 358  k 359  t 360  f 361  p 362  k 363  k 364  e 365  k 366  k 367  q 368  k 369  a 370  p 371  k 372  e 373  e 374  s 375  t 376  d 377   
               
               
                   
               
               
                 q 378  m 379  s 380  e 381  p 382  p 383  k 384  e 385  q 386  r 387  v 388  q 389  g 390  s 391  i 392  t 393  q 394  r 395  t 396  r 397  p 398  s 399  V 400  q 401   
               
               
                   
               
               
                 p 402  g 403  p 404  m 405  p 406  m 407  i 408  d 409  v 410  n 411  t 412  d 413   
               
               
                   
               
               
                 Amino-terminal 
               
               
                 (SEQ ID NO: 211) 
                   
               
               
                 h 50 _g 51 _k 52 _v 53 _p 54 _l 55 _t 56 _f 57 _p 58 _p 59 _g 60 _q 61 _g 62 _v 63 _p 64 _l 65 _n 66 _a 67 _n 68 _s 69 _t 70 _p 71 _a 72 _q 73 _n 74 _a 75 _g 76 _y 77   
                   
               
               
                 w 78 _r 79 _r 80 _q 81 _d 82 _r 83 _k 84 _i 85 _n 86 _t 87 _g 88 _n 89 _g 90 _i 91 _k 92   
               
               
                   
               
               
                 (SEQ ID NO: 212) 
                   
               
               
                 k 84 _i 85 _n 86 _t 87 _g 88 _n 89 _g 90 _i 91 _k 92 __l 94 _a 95 _p 96 _r 97 _w 98 _y 99 _f 100 _y 101 _y 102 _t 103 _g 104 _t 105 _g 106 _p 107 _e 108 _a 109   
                   
               
               
                 a 110 _l 111 _p 112 _f 113 _r 114 _a 115 _v 116 _k 117 _d 118 _g 119 _i 120 _v 121 _w 122 _v 123 _h 124   
               
               
                   
               
               
                 Mid-molecule 
               
               
                 (SEQ ID NO: 213) 
                   
               
               
                 k 229 _v 230 _k 231 _q 232 _s 233 _q 234 _p 235 _k 236 _v 237 _i 238 _t 239 _k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251   
                   
               
               
                 h 252   
               
               
                   
               
               
                 (SEQ ID NO: 214) 
                   
               
               
                 k 231 _q 232 _s 233 _q 234 _p 235 _k 236 _v 237 _i 238 _t 239 _k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 215) 
                   
               
               
                 k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 24) 
                   
               
               
                 k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 22) 
                   
               
               
                 k 247 _a 248 _k 249 _m 250 _r 251 _h 252 _k 253   
                   
               
               
                   
               
               
                 (SEQ ID NO: 25) 
                   
               
               
                 k 249 _m 250 _r 251 _h 252 _k 253 _r 254 _t 255 _s 256 _t 257 _k 258   
                   
               
               
                   
               
               
                 Carboxy-terminal 
               
               
                 (SEQ ID NO: 216) 
                   
               
               
                 h 316 _q 317 _n 318 _n 319 _d 320 _d 321 _h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338   
                   
               
               
                 p 339 _k 340 _n 341 _p3 42 _n 343 _y 344 _n 345 _k 346   
               
               
                   
               
               
                 (SEQ ID NO: 217) 
                   
               
               
                 h 316 _q 317 _n 318 _n 319 _d 320 _d 321 _h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338   
                   
               
               
                 p 339 _k 340 _n 341 _p3 42 _n 343 _y 344 _n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361   
               
               
                 p 362 _k 363 _k 364 _e 365 _k 366   
               
               
                   
               
               
                 (SEQ ID NO: 218) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346   
               
               
                   
               
               
                 (SEQ ID NO: 219) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366   
               
               
                   
               
               
                 (SEQ ID NO: 220) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                   
               
               
                 (SEQ ID NO: 221) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                 q 368 _k 369   
               
               
                   
               
               
                 (SEQ ID NO: 222) 
                   
               
               
                 h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                 q 368 _k 369 _a 370 _p 371 _k 372   
               
               
                   
               
               
                 Replikin Count in AFY13314 is the number of Replikin sequences per 100 amino acid 
               
               
                 residues, which is 15/413 and equals 3.6. 
               
               
                   
               
               
                 (SEQ ID NO: 22) 
                   
               
               
                 KNKMRHK  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188586 position 247, AGV08386 position 247, AGV08591 position 247, 
               
               
                   
               
               
                 AGV08415 position 247, AFY13314 position 247, AGG22549 position 247, AGH58724 
               
               
                   
               
               
                 position 247, AFS88943 position 247. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22571 position 247, AHY22561 position 247, AHY22551 position 247, AHY22541 
               
               
                   
               
               
                 position 247, AHY22531 position 247, AHE78112 position 247, AHE78101 position 247, 
               
               
                   
               
               
                 AHX00738 position 247, AHX00728 position 247, AHX00718 position 247, AHN10819 
               
               
                   
               
               
                 position 247, AHI48838 position 247, AHI48819 position 247, AHI48812 position 247, 
               
               
                   
               
               
                 AHI48810 position 247, AHI48808 position 247, AHI48806 position 247, AHI48804 
               
               
                   
               
               
                 position 247, AHI48718 position 247, AHI48709 position 247, AHI48699 position 247, 
               
               
                   
               
               
                 AHI48689 position 247, AHI48679 position 247, AHI48669 position 247, AHI48659 
               
               
                   
               
               
                 position 247, AHI48649 position 247, AHI48647 position 247, AHI48644 position 247, 
               
               
                   
               
               
                 AHI48641 position 247, AHI48638 position 247, AHI48635 position 247, AHI48633 
               
               
                   
               
               
                 position 247, AHI48623 position 247, AHI48612 position 247, AHI48601 position 247, 
               
               
                   
               
               
                 AHI48590 position 247, AHI48579 position 247, AHI48568 position 247, AHI48557 
               
               
                   
               
               
                 position 247, AHI48546 position 247, AHI48535 position 247, AHI48524 position 247, 
               
               
                   
               
               
                 AHL18098 position 247, AHC74105 position 247, AHC74095 position 247, AHB33333 
               
               
                   
               
               
                 position 247, K9N4V7 position 247, AGV08580 position 247, AGV08565 position 247, 
               
               
                   
               
               
                 AGV08553 position 247, AGV08542 position 247, AGV08531 position 247, AGV08517 
               
               
                   
               
               
                 position 247, AGV08512 position 247, AGV08502 position 247, AGV08499 position 247, 
               
               
                   
               
               
                 AGV08487 position 247, AGV08474 position 247, AGV08462 position 247, AGV08451 
               
               
                   
               
               
                 position 247, AGV08433 position 247, AGV08418 position 247, AGV08397 position 247, 
               
               
                   
               
               
                 AGN72646 position 247, AGN70980 position 247, AGN70970 position 247, AGN70958 
               
               
                   
               
               
                 position 247, AGN70936 position 247. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21476 position 247, AHX71953 position 247. 
               
               
                   
               
               
                 AHN10819 is a from human host in Saudi Arabia 
               
            
           
         
       
     
     Example 8 
     Analysis of Accession No. AGV08386 for Replikin Sequences Conserved in Human Host and Shared with Isolates in Camel Hosts 
       
     
       
         
           
               
               
            
               
                 PubMed Code: 
                   
               
               
                 AGV08386 
               
               
                   
               
               
                 Description: 
               
               
                 Spread, circulation, and evolution of the Middle East respiratory syndrome coronavirus 
               
               
                 Direct Submission 
               
               
                   
               
               
                 Isolated: 
               
               
                 2012 in Saudi Arabia 
               
               
                   
               
               
                 Source: 
               
               
                 Middle East respiratory syndrome coronavirus (MERS-CoV) 
               
               
                   
               
               
                 Strain: 
               
               
                 MERS-CoV 
               
               
                 (SEQ ID NO: 223) 
                   
               
               
                 m 1  a 2  s 3  p 4  a 5  a 6  p 7  r 8  a 9  v 10  s 11  f 12  a 13  y 14  n 15  n 16  d 17  i 18  t 19  n 20  t 21  n 22  l 23  s 24  r 25  g 26  r 27  g 28  r 29  n 30  p 31   
                   
               
               
                   
               
               
                 k 32  p 33  r 34  a 35  a 36  p 37  n 38  n 39  t 40  v 41  s 42  w 43  y 44  t 45  g 46  l 47  t 48  q 49  h 50  g 51  k 52  v 53  p 54  l 55  t 56  f 57  p 58  p 59  g 60   
               
               
                   
               
               
                 q 61  g 62  v 63  p 64  l 65  n 66  a 67  n 68  s 69  t 70  p 71  a 72  q 73  n 74  a 75  g 76  y 77  w 78  r 79  r 80  q 81  d 82  r 83  k 84  i 85  n 86  t 87  g 88  n 89   
               
               
                   
               
               
                 g 90  i 91  k 92  q 93  l 94  a 95  p 96  r 97  w 98  y 99  f 100  y 101  y 102  t 103  g 104  t 105  g 106  p 107  e 108  a 109  a 110  l 111  p 112  f 113  r 114  a 115   
               
               
                   
               
               
                 v 116  k 117  d 118  g 119  i 120  v 121  w 122  v 123  h 124  e 125  d 126  g 127  a 128  t 129  d 130  a 131  p 132  s 133  t 134  f 135  g 136  t 137  r 138  n 139   
               
               
                   
               
               
                 p 140  n 141  n 142  d 143  s 144  a 145  i 146  v 147  t 148  q 149  f 150  a 151  p 152  g 153  t 154  k 155  l 156  p 157  k 158  n 159  f 160  h 161  i 162  e 163   
               
               
                   
               
               
                 g 164  t 165  g 166  g 167  n 168  s 169  q 170  s 171  s 172  s 173  r 174  a 175  s 176  s 177  v 178  s 179  r 180  n 181  s 182  s 183  r 184  s 185  s 186  s 187   
               
               
                   
               
               
                 q 188  g 189  s 190  r 191  s 192  g 193  n 194  s 195  t 196  r 197  g 198  t 199  s 200  p 201  g 202  p 203  s 204  g 205  i 206  g 207  a 208  v 209  g 210  g 211   
               
               
                   
               
               
                 d 212  l 213  l 214  y 215  l 216  d 217  l 218  l 219  n 220  r 221  l 222  q 223  a 224  l 225  e 226  s 227  g 228  k 229  v 230  k 231  q 232  s 233  q 234  p 235   
               
               
                   
               
               
                 k 236  v 237  i 238  t 239  k 240  k 241  d 242  a 243  a 244  a 245  a 246  k 247  n 248  k 249  m 250  r 251  h 252  k 253  r 254  t 255  s 256  t 257  k 258  s 259   
               
               
                   
               
               
                 f 260  n 261  m 262  v 263  q 264  a 265  f 266  g 267  l 268  r 269  g 270  p 271  g 272  d 273  l 274  q 275  g 276  n 277  f 278  g 279  d 280  l 281  q 282  l 283   
               
               
                   
               
               
                 n 284  k 285  l 286  g 287  t 288  e 289  d 290  p 291  r 292  w 293  p 294  q 295  i 296  a 297  e 298  l 299  a 300  p 301  t 302  a 303  s 304  a 305  f 306  m 307   
               
               
                   
               
               
                 g 308  m 309  s 310  q 311  f 312  k 313  l 314  t 315  h 316  q 317  n 318  n 319  d 320  d 321  h 322  g 323  n 324  p 325  v 326  y 327  f 328  l 329  r 330  y 331   
               
               
                   
               
               
                 s 332  g 333  a 334  i 335  k 336  l 337  d 338  p 339  k 340  p 341  p 342  n 343  y 344  p 345  k 346  w 347  l 348  e 349  l 350  l 351  e 352  q 353  n 354  i 355   
               
               
                   
               
               
                 d 356  a 357  y 358  k 359  t 360  f 361  p 362  k 363  k 364  e 365  k 366  k 367  q 368  k 369  a 370  p 371  k 372  e 373  e 374  s 375  t 376  d 377  q 378  m 379   
               
               
                   
               
               
                 s 380  e 381  p 382  p 383  k 384  e 385  q 386  r 387  v 388  q 389  g 390  s 391  i 392  t 393  q 394  r 395  t 396  r 397  p 398  s 399  V 400  q 401  p 402  g 403   
               
               
                   
               
               
                 p 404  g 405  p 406  m 407  i 408  d 409  v 410  n 411  t 412  d 413   
               
               
                   
               
               
                 (SEQ ID NO: 224) 
                   
               
               
                 h 50 _g 51 _k 52 _v 53 _p 54 _l 55 _t 56 _f 57 _p 58 _p 59 _g 60 _q 61 _g 62 _v 63 _p 64 _l 65 _n 66 _a 67 _n 68 _s 69 _t 70 _p 71 _a 72 _q 73 _n 74 _a 75 _g 76 _y 77   
                   
               
               
                 w 78 _r 79 _r 80 _q 81 _d 82 _r 83 _k 84 _i 85 _n 86 _t 87 _g 88 _n 89 _g 90 _i 91 _k 92   
               
               
                   
               
               
                 (SEQ ID NO: 225) 
                   
               
               
                 k 84 _i 85 _n 86 _t 87 _g 88 _n 89 _g 90 _i 91 _k 92 __l 94 _a 95 _p 96 _r 97 _w 98 _y 99 _f 100 _y 101 _y 102 _t 103 _g 104 _t 105 _g 106 _p 107 _e 108 _a 109   
                   
               
               
                 a 110 _l 111 _p 112 _f 113 _r 114 _a 115 _v 116 _k 117 _d 118 _g 119 _i 120 _v 121 _w 122 _v 123 _h 124   
               
               
                   
               
               
                 Mid-molecule 
               
               
                 (SEQ ID NO: 226) 
                   
               
               
                 k 229 _v 230 _k 231 _q 232 _s 233 _q 234 _p 235 _k 236 _v 237 _i 238 _t 239 _k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251   
                   
               
               
                 h 252   
               
               
                   
               
               
                 (SEQ ID NO: 227) 
                   
               
               
                 k 231 _q 232 _s 233 _q 234 _p 235 _k 236 _v 237 _i 238 _t 239 _k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 228) 
                   
               
               
                 k 240 _k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 24) 
                   
               
               
                 k 241 _d 242 _a 243 _a 244 _a 245 _a 246 _k 247 _n 248 _k 249 _m 250 _r 251 _h 252   
                   
               
               
                   
               
               
                 (SEQ ID NO: 22) 
                   
               
               
                 k 247 _a 248 _k 249 _m 250 _r 251 _h 252 _k 253   
                   
               
               
                   
               
               
                 (SEQ ID NO: 25) 
                   
               
               
                 k 249 _m 250 _r 251 _h 252 _k 253 _r 254 _t 255 _s 256 _t 257 _k 258   
                   
               
               
                   
               
               
                 Carboxy-terminal 
               
               
                 (SEQ ID NO: 229) 
                   
               
               
                 h 316 _q 317 _n 318 _n 319 _d 320 _d 321 _h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338   
                   
               
               
                 p 339 _k 340 _n 341 _p 342 _n 343 _y 344 _n 345 _k 346   
               
               
                   
               
               
                 (SEQ ID NO: 230) 
                   
               
               
                 h 316 _q 317 _n 318 _n 319 _d 320 _d 321 _h 322 _g 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338   
                   
               
               
                 p 339 _k 340 _n 341 _p 342 _n 343 _y 344 _n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361   
               
               
                 p 362 _k 363 _k 364 _e 365 _k 366   
               
               
                   
               
               
                 (SEQ ID NO: 231) 
                   
               
               
                 h 322 _q 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346   
               
               
                   
               
               
                 (SEQ ID NO: 232) 
                   
               
               
                 h 322 _q 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366   
               
               
                   
               
               
                 (SEQ ID NO: 233) 
                   
               
               
                 h 322 _q 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                   
               
               
                 (SEQ ID NO: 234) 
                   
               
               
                 h 322 _q 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                 q 368 _k 369   
               
               
                   
               
               
                 (SEQ ID NO: 235) 
                   
               
               
                 h 322 _q 323 _n 324 _p 325 _v 326 _y 327 _f 328 _l 329 _r 330 _y 331 _s 332 _g 333 _a 334 _i 335 _k 336 _l 337 _d 338 _p 339 _k 340 _n 341 _p 342 _n 343 _y 344   
                   
               
               
                 n 345 _k 346 _w 347 _l 348 _e 349 _l 350 _l 351 _e 352 _q 353 _n 354 _i 355 _d 356 _a 357 _y 358 _k 359 _t 360 _f 361 _p 362 _k 363 _k 364 _e 365 _k 366 _k 367   
               
               
                 q 368 _k 369 _a 370 _p 371 _k 372   
               
               
                   
               
               
                 Replikin Count in AGV08386 is the number of Replikin sequences per 100 amino acid 
               
               
                 residues, which is 15/413 and equals 3.6. 
               
            
           
         
       
     
     Example 9 
     Analysis of Accession No. AGV08441 for Replikin Sequences Conserved in Human Host and Shared with Isolates in Camel Hosts 
       
     
       
         
           
               
               
            
               
                 AGV08441 
                   
               
               
                 2013 Saudi Arabia 
               
               
                   
               
               
                 Source: 
               
               
                 Middle East respiratory syndrome coronavirus (MERS-CoV) 
               
               
                   
               
               
                 Strain: 
               
               
                 MERS-CoV 
               
               
                 (SEQ ID NO: 236) 
                   
               
               
                 m 1  e 2  e 3  s 4  l 5  m 6  d 7  v 8  p 9  s 10  t 11  s 12  g 13  t 14  q 15  v 16  y 17  s 18  r 19  k 20  a 21  r 22  k 23  r 24  s 25  h 26  s 27  p 28  t 29   
                   
               
               
                 k 30  k 31  l 32  r 33  y 34  v 35  k 36  r 37  r 38  f 39  s 40  l 41  l 42  r 43  p 44  e 45  d 46  l 47  s 48  v 49  i 50  v 51  q 52  p 53  t 54  h 55  y 56   
               
               
                 v 57  r 58  v 59  t 60  f 61  s 62  d 63  p 64  a 65  m 66  w 67  y 68  l 69   
               
               
                   
               
               
                 Amino-terminal 
               
               
                 (SEQ ID NO: 5) 
                   
               
               
                 k 20 _a 21 _r 22 _k 23 _r 24 _s 25 _h 26 _s 27 _p 28 _t 29 _k 30   
                   
               
               
                   
               
               
                 (SEQ ID NO: 237) 
                   
               
               
                 k 20 _a 21 _r 22 _k 23 _r 24 _s 25 _h 26 _s 27 _p 28 _t 29 _k 30 _k 31 _l 32 _r 33 _y 34 _v 35 _k 36 _r 37 _r 38 _f 39 _s 40 _l 41 _l 42 _r 43 _p 44 _e 45 _d 46 _l 47 _ 
                   
               
               
                 s 48 _v 49 _i 50 _v 51 _q 52 _p 53 _t 54 _h 55   
               
               
                   
               
               
                 (SEQ ID NO: 238) 
                   
               
               
                 k 23 _r 24 _s 25 _h 26 _s 27 _p 28 _t 29 _k 30 _k 31 _l 32 _r 33 _y 34 _v 35 _k 36 _r 37 _r 38 _f 39 _s 40 _l 41 _l 42 _r 43 _p 44 _e 45 _d 46 _l 47 _s 48 _v 49 _i 50 _v 51 _ 
                   
               
               
                 q 52 _p 53 _t 54 _h 55   
               
               
                   
               
               
                 (SEQ ID NO: 3) 
                   
               
               
                 k 23 _r 24 _s 25 _h 26 _s 27 _p 28 _t 29 _k 30   
                   
               
               
                   
               
               
                 (SEQ ID NO: 23) 
                   
               
               
                 k 23 _r 24 _s 25 _h 26 _s 27 _p 28 _t 29   
                   
               
               
                   
               
               
                 Mid-molecule 
               
               
                 (SEQ ID NO: 4) 
                   
               
               
                 h 26 _s 27 _p 28 _t 29 _k 30 _k 31 _l 32 _r 33 _y 34 _v 35   
                   
               
               
                   
               
               
                 (SEQ ID NO: 239) 
                   
               
               
                 k 30 _k 31 _l 32 _r 33 _y 34 _v 35 _k 36 _r 37 _r 38 _f 39 _s 40 _l 41 _l 42 _r 43 _p 44 _e 45 _d 46 _l 47 _s 48 _v 49 _i 50 _v 51 _q 52 _p 53 _t 54 _h 55   
                   
               
               
                   
               
               
                 Carboxy-terminal 
               
               
                 Zero Replikins. 
               
               
                   
               
               
                 Replikin Count in AFY13314 is the number of Replikin sequences per 100 amino acid 
               
               
                 residues, which is 7/69 and equals 10.1. 
               
               
                   
               
               
                 Sequence History by Year for Replikin Sequences Identified in Accession No. 
               
               
                 AGV08441 
               
               
                   
               
               
                 (SEQ ID NO: 5) 
                   
               
               
                 KARKRSHSPTK  
                   
               
               
                 (identified in, for example, camel Qatar 2014 and in 
               
               
                 human Saudi Arabia AHN10815 after exposure to infected camel;  
               
               
                   
               
               
                 (SEQ ID NO: 23) 
                   
               
               
                 KRSHSPTK  
                   
               
               
                 is another Replikin sequence identified within SEQ ID NO: 5) 
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188582 position 20, AGV08382 position 20, AGV08587 position 20, AGV08411 
               
               
                   
               
               
                 position 20, AFY13310 position 20, AGG22545 position 20, AFS88939 position 20. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22567 position 20, AHY22557 position 20, AHY22547 position 20, AHY22537 
               
               
                   
               
               
                 position 20, AHY22527 position 20, AHY04437 position 20, AHE78113 position 20, 
               
               
                   
               
               
                 AHE78102 position 20, AHN10815 position 20, AHI48842 position 20, AHI48834 
               
               
                   
               
               
                 position 20, AHI48827 position 20, AHI48821 position 20, AHI48815 position 20, 
               
               
                   
               
               
                 AHI48714 position 20, AHI48705 position 20, AHI48695 position 20, AHI48685 position 
               
               
                   
               
               
                 20, AHI48675 position 20, AHI48665 position 20, AHI48655 position 20, AHI48629 
               
               
                   
               
               
                 position 20, AHI48619 position 20, AHI48608 position 20, AHI48597 position 20, 
               
               
                   
               
               
                 AHI48586 position 20, AHI48575 position 20, AHI48564 position 20, AHI48553 position 
               
               
                   
               
               
                 20, AHI48542 position 20, AHI48531 position 20, AHI48520 position 20, AHL18094 
               
               
                   
               
               
                 position 32, AHC74101 position 20, AHC74091 position 20, AHB33329 position 20, 
               
               
                   
               
               
                 AGV08576 position 20, AGV08561 position 20, AGV08549 position 20, AGV08538 
               
               
                   
               
               
                 position 20, AGV08527 position 20, AGV08508 position 20, AGV08495 position 20, 
               
               
                   
               
               
                 AGV08483 position 20, AGV08470 position 20, AGV08458 position 20, AGV08447 
               
               
                   
               
               
                 position 20, AGV08441 position 20, AGV08429 position 20, AGV08423 position 20, 
               
               
                   
               
               
                 AGV08393 position 20, K9N643 position 20, AGN72642 position 20, AGN70976 position 
               
               
                   
               
               
                 20, AGN70965 position 20, AGN70954 position 20, AGN70932 position 20. 
               
               
                   
               
               
                 2014 
               
               
                 AHX71949 position 20 . 
               
               
                   
               
               
                 (SEQ ID NO: 3) 
                   
               
               
                 KRSHSPTKK  
                   
               
               
                 (identified in, for example, camel in Qatar 2014) 
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188582 position 23, AGV08382 position 23, AGV08587 position 23, AGV08411 
               
               
                   
               
               
                 position 23, AFY13310 position 23, AGG22545 position 23, AFS88939 position 23. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22567 position 23, AHY22557 position 23, AHY22547 position 23, AHY22537 
               
               
                   
               
               
                 position 23, AHY2252 position 23, AHY04437 position 23, AHE78113 position 23, 
               
               
                   
               
               
                 AHE78102 position 23, AHN10815 position 23, AHI48842 position 23, AHI48834 
               
               
                   
               
               
                 position 23, AHI48827 position 23, AHI48821 position 23, AHI48815 position 23, 
               
               
                   
               
               
                 AHI48714 position 23, AHI48705 position 23, AHI48695 position 23, AHI48685 position 
               
               
                   
               
               
                 23, AHI48675 position 23, AHI48665 position 23, AHI48655 position 23, AHI48629 
               
               
                   
               
               
                 position 23, AHI48619 position 23, AHI48608 position 23, AHI48597 position 23, 
               
               
                   
               
               
                 AHI48586 position 23, AHI48575 position 23, AHI48564 position 23, AHI48553 position 
               
               
                   
               
               
                 23, AHI48542 position 23, AHI48531 position 23, AHI48520 position 23, AHL18094 
               
               
                   
               
               
                 position 35, AHC74101 position 23, AHC74091 position 23, AHC74084 position 23, 
               
               
                   
               
               
                 AHB33329 position 23, AGV08576 position 23, AGV08561 position 23, AGV08549 
               
               
                   
               
               
                 position 23, AGV08538 position 23, AGV08527 position 23, AGV08508 position 23, 
               
               
                   
               
               
                 AGV08495 position 23, AGV08483 position 23, AGV08470 position 23, AGV08458 
               
               
                   
               
               
                 position 23, AGV08447 position 23, AGV08441 position 23, AGV08429 position 23, 
               
               
                   
               
               
                 AGV08423 position 23, AGV08393 position 23, K9N643 position 23, AGN72642 position 
               
               
                   
               
               
                 23, AGN70976 position 23, AGN70965 position 23, AGN70954 position 23, AGN70932 
               
               
                   
               
               
                 position 23. 
               
               
                   
               
               
                 2014 
               
               
                 AHX71949 position 23. 
               
               
                   
               
               
                 (SEQ ID NO: 4) 
                   
               
               
                 HSPTKKLRYVK  
                   
               
               
                 (identified in, for example, camel in Qatar 2014) 
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188582 position 26, AGV08382 position 26, AGV08587 position 26, AGV08411 
               
               
                   
               
               
                 position 26, AFY13310 position 26, AGG22545 position 26, AGH58721 position 26, 
               
               
                   
               
               
                 AFS88939 position 26. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22567 position 26, AHY22557 position 26, AHY22547 position 26, AHY22537 
               
               
                   
               
               
                 position 26, AHY22527 position 26, AHY04437 position 26, AHE78113 position 26, 
               
               
                   
               
               
                 AHE78102 position 26, AHX00734 position 26, AHX00724 position 26, AHX00714 
               
               
                   
               
               
                 position 26, AHN10815 position 26, AHI48842 position 26, AHI48834 position 26, 
               
               
                   
               
               
                 AHI48827 position 26, AHI48821 position 26, AHI48815 position 26, AHI48714 position 
               
               
                   
               
               
                 26, AHI48705 position 26, AHI48695 position 26, AHI48685 position 26, AHI48675 
               
               
                   
               
               
                 position 26, AHI48665 position 26, AHI48655 position 26, AHI48629 position 26, 
               
               
                   
               
               
                 AHI48619 position 26, AHI48608 position 26, AHI48597 position 26, AHI48586 position 
               
               
                   
               
               
                 26, AHI48575 position 26, AHI48564 position 26, AHI48553 position 26, AHI48542 
               
               
                   
               
               
                 position 26, AHI48531 position 26, AHI48520 position 26, AHL18094 position 38, 
               
               
                   
               
               
                 AHC74101 position 26, AHC74091 position 26, AHC74084 position 26, AHB33329 
               
               
                   
               
               
                 position 26, AGV08576 position 26, AGV08561 position 26, AGV08549 position 26, 
               
               
                   
               
               
                 AGV08538 position 26, AGV08527 position 26, AGV08508 position 26, AGV08495 
               
               
                   
               
               
                 position 26, AGV08483 position 26, AGV08470 position 26, AGV08458 position 26, 
               
               
                   
               
               
                 AGV08447 position 26, AGV08441 position 26, AGV08429 position 26, AGV08423 
               
               
                   
               
               
                 position 26, AGV08393 position 26, K9N643 position 26, AGN72642 position 26, 
               
               
                   
               
               
                 AGN70976 position 26, AGN70965 position 26, AGN70954 position 26, AGN70932 
               
               
                   
               
               
                 position 26. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21472 position 26, AHX71949 position 26. 
               
            
           
         
       
     
     Example 10 
     Analysis of Accession No. K9N4V7 (Betacoronavirus England 1) for Replikin Sequences Conserved in Human Host and Shared with Isolates in Camel Hosts 
       
     
       
         
           
               
               
            
               
                 (SEQ ID NO: 24) 
                   
               
               
                 KDAAAAKNKMRH  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188586 position 241, AGV08386 position 241, AGV08591 position 241, 
               
               
                   
               
               
                 AGV08415 position 241, AFY13314 position 241, AGG22549 position 241, AGH58724 
               
               
                   
               
               
                 position 241, AFS88943 position 241 . 
               
               
                   
               
               
                 2013 
               
               
                 AHY22571 position 241, AHY22561 position 241, AHY22551 position 241, AHY22541 
               
               
                   
               
               
                 position 241, AHY22531 position 241, AHE78112 position 241, AHE78101 position 241, 
               
               
                   
               
               
                 AHX00738 position 241, AHX00728 position 241, AHX00718 position 241, AHN10819 
               
               
                   
               
               
                 position 241, AHI48838 position 241, AHI48819 position 241, AHI48812 position 241, 
               
               
                   
               
               
                 AHI48810 position 241, AHI48808 position 241, AHI48806 position 241, AHI48804 
               
               
                   
               
               
                 position 241, AHI48718 position 241, AHI48709 position 241, AHI48699 position 241, 
               
               
                   
               
               
                 AHI48689 position 241, AHI48679 position 241, AHI48669 position 241, AHI48659 
               
               
                   
               
               
                 position 241, AHI48649 position 241, AHI48647 position 241, AHI48644 position 241, 
               
               
                   
               
               
                 AHI48641 position 241, AHI48638 position 241, AHI48635 position 241, AHI48633 
               
               
                   
               
               
                 position 241, AHI48623 position 241, AHI48612 position 241, AHI48601 position 241, 
               
               
                   
               
               
                 AHI48590 position 241, AHI48579 position 241, AHI48568 position 241, AHI48557 
               
               
                   
               
               
                 position 241, AHI48546 position 241, AHI48535 position 241, AHI48524 position 241, 
               
               
                   
               
               
                 AHL18098 position 241, AHC74105 position 241, AHC74095 position 241, AHB33333 
               
               
                   
               
               
                 position 241, K9N4V7 position 241, AGV08580 position 241, AGV08565 position 241, 
               
               
                   
               
               
                 AGV08553 position 241, AGV08542 position 241, AGV08531 position 241, AGV08517 
               
               
                   
               
               
                 position 241, AGV08512 position 241, AGV08502 position 241, AGV08499 position 241, 
               
               
                   
               
               
                 AGV08487 position 241, AGV08474 position 241, AGV08462 position 241, AGV08451 
               
               
                   
               
               
                 position 241, AGV08433 position 241, AGV08418 position 241, AGV08397 position 241, 
               
               
                   
               
               
                 AGN72646 position 241, AGN70980 position 241, AGN70970 position 241, AGN70958 
               
               
                   
               
               
                 position 241, AGN70936 position 241. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21476 position 241, AHX71953 position 241. 
               
               
                   
               
               
                 (SEQ ID NO: 25) 
                   
               
               
                 KMRHKRTSTK  
                   
               
               
                   
               
               
                 All occurrences of the sequence by year: 
               
               
                 2012 
               
               
                 YP_007188586 position 249, AGV08386 position 249, AGV08591 position 249, 
               
               
                   
               
               
                 AGV08415 position 249, AFY13314 position 249, AGG22549 position 249, AGH58724 
               
               
                   
               
               
                 position 249, AFS88943 position 249. 
               
               
                   
               
               
                 2013 
               
               
                 AHY22571 position 249, AHY22561 position 249, AHY22551 position 249, AHY22541 
               
               
                   
               
               
                 position 249, AHY22531 position 249, AHE78112 position 249, AHE78101 position 249, 
               
               
                   
               
               
                 AHX00738 position 249, AHX00728 position 249, AHX00718 position 249, AHN10819 
               
               
                   
               
               
                 position 249, AHI48838 position 249, AHI48819 position 249, AHI48812 position 249, 
               
               
                   
               
               
                 AHI48810 position 249, AHI48808 position 249, AHI48806 position 249, AHI48804 
               
               
                   
               
               
                 position 249, AHI48718 position 249, AHI48709 position 249, AHI48699 position 249, 
               
               
                   
               
               
                 AHI48689 position 249, AHI48679 position 249, AHI48669 position 249, AHI48659 
               
               
                   
               
               
                 position 249, AHI48649 position 249, AHI48647 position 249, AHI48644 position 249, 
               
               
                   
               
               
                 AHI48641 position 249, AHI48638 position 249, AHI48635 position 249, AHI48633 
               
               
                   
               
               
                 position 249, AHI48623 position 249, AHI48612 position 249, AHI48601 position 249, 
               
               
                   
               
               
                 AHI48590 position 249, AHI48579 position 249, AHI48568 position 249, AHI48557 
               
               
                   
               
               
                 position 249, AHI48546 position 249, AHI48535 position 249, AHI48524 position 249, 
               
               
                   
               
               
                 AHL18098 position 249, AHC74105 position 249, AHC74095 position 249, AHB33333 
               
               
                   
               
               
                 position 249, K9N4V7 position 249, AGV08580 position 249, AGV08565 position 249, 
               
               
                   
               
               
                 AGV08553 position 249, AGV08542 position 249, AGV08531 position 249, AGV08517 
               
               
                   
               
               
                 position 249, AGV08512 position 249, AGV08502 position 249, AGV08499 position 249, 
               
               
                   
               
               
                 AGV08487 position 249, AGV08474 position 249, AGV08462 position 249, AGV08451 
               
               
                   
               
               
                 position 249, AGV08433 position 249, AGV08418 position 249, AGV08397 position 249, 
               
               
                   
               
               
                 AGN72646 position 249, AGN70980 position 249, AGN70970 position 249, AGN70958 
               
               
                   
               
               
                 position 249, AGN70936 position 249. 
               
               
                   
               
               
                 2014 
               
               
                 AHY21476 position 249, AHX71953 position 249. 
               
            
           
         
       
     
     Example 11 
     Replikin Concentration by Year for Isolates of MERS-CoV from 2012 Through 2014 
       
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                   
                   
                   
                 Mean 
                   
                   
               
               
                   
                   
                   
                 Replikin 
                   
                   
               
               
                   
                   
                 No. of 
                 Count 
                   
                   
               
               
                   
                   
                 Isolates 
                 per 
                   
                   
               
               
                 Year 
                 PubMed Accession Number-Replikin Count 
                 per year 
                 year 
                 S.D. 
                 Significance 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 2012 
                 YP_007188577 162 YP_007188586 21 YP_007188585 3 
                 52 
                 2.9 
                 1.7 
                 low p &gt; .50 
               
               
                   
                 YP_007188582 12 YP_007188579 13 YP_007188578 100 
                   
                   
                   
                   
               
               
                   
                 AGV08386 15 AGV08385 3 AGV08382 12 AGV08379 13 
                   
                   
                   
                   
               
               
                   
                 AGV08378 100 AGV08377 162 AGV08591 15 AGV08590 3 
                   
                   
                   
                   
               
               
                   
                 AGV08587 12 AGV08584 13 AGV08583 100 AGV08582 162 
                   
                   
                   
                   
               
               
                   
                 AGV08415 15 AGV08414 3 AGV08411 12 AGV08408 13 
                   
                   
                   
                   
               
               
                   
                 AGV08407 100 AGV08406 162 AFY13314 21 AFY13313 3 
                   
                   
                   
                   
               
               
                   
                 AFY13310 12 AFY13307 13 AFY13306 162 AFY13305 100 
                   
                   
                   
                   
               
               
                   
                 AGO06003 6 AGO06001 2 AGO06000 4 AGO05997 2 
                   
                   
                   
                   
               
               
                   
                 AGG22549 21 AGG22548 3 AGG22545 12 AGG22542 13 
                   
                   
                   
                   
               
               
                   
                 AGG22541 100 AGG22540 162 AGH58724 15 AGH58721 12 
                   
                   
                   
                   
               
               
                   
                 AGH58718 3 AGH58717 13 AGH58716 160 AGH58715 98 
                   
                   
                   
                   
               
               
                   
                 AFV09327 99 AFS88944 161 AFS88943 15 AFS88942 3 
                   
                   
                   
                   
               
               
                   
                 AFS88939 15 AFS88936 13 
                   
                   
                   
                   
               
               
                 2013 
                 AHY22571 13 AHY22570 3 AHY22567 12 AHY22565 13 
                 501 
                 2.4 
                 1.4 
                 low p &gt; .50, 
               
               
                   
                 AHY22564 100 AHY22563 162 AHY22561 13 AHY22560 3 
                   
                   
                   
                 prev p &lt; .05 
               
               
                   
                 AHY22557 12 AHY22555 13 AHY22554 100 AHY22553 162 
                   
                   
                   
                   
               
               
                   
                 AHY22551 15 AHY22550 3 AHY22547 12 AHY22545 13 
                   
                   
                   
                   
               
               
                   
                 AHY22544 100 AHY22543 165 AHY22541 16 AHY22540 3 
                   
                   
                   
                   
               
               
                   
                 AHY22537 12 AHY22535 13 AHY22534 100 AHY22533 162 
                   
                   
                   
                   
               
               
                   
                 AHY22531 16 AHY22530 3 AHY22527 12 AHY22525 13 
                   
                   
                   
                   
               
               
                   
                 AHY22524 100 AHY22523 162 AHY04437 12 AHY04436 4 
                   
                   
                   
                   
               
               
                   
                 AHY04435 2 AHY04434 2 AHY04433 6 AHX71943 2 
                   
                   
                   
                   
               
               
                   
                 AHX71942 2 AHX71941 4 AHX71940 6 AHE78113 12 
                   
                   
                   
                   
               
               
                   
                 AHE78112 15 AHE78111 3 AHE78108 13 AHE78107 98 
                   
                   
                   
                   
               
               
                   
                 AHE78106 160 AHE78102 12 AHE78101 15 AHE78100 3 
                   
                   
                   
                   
               
               
                   
                 AHE78097 13 AHE78096 98 AHE78095 160 AHX00738 15 
                   
                   
                   
                   
               
               
                   
                 AHX00737 3 AHX00734 8 AHX00731 13 AHX00730 162 
                   
                   
                   
                   
               
               
                   
                 AHX00729 100 AHX00728 15 AHX00727 3 AHX00724 8 
                   
                   
                   
                   
               
               
                   
                 AHX00721 13 AHX00720 162 AHX00719 100 AHX00718 15 
                   
                   
                   
                   
               
               
                   
                 AHX00717 3 AHX00714 8 AHX00711 13 AHX00710 162 
                   
                   
                   
                   
               
               
                   
                 AHX00709 100 K9N638 100 K9N7C7 162 AHN10826 1 
                   
                   
                   
                   
               
               
                   
                 AHN10825 2 AHN10824 4 AHN10819 15 AHN10818 3 
                   
                   
                   
                   
               
               
                   
                 AHN10815 12 AHN10812 13 AHN10811 98 AHN10810 148 
                   
                   
                   
                   
               
               
                   
                 AHI48842 12 AHI48838 15 AHI48837 3 AHI48834 12 
                   
                   
                   
                   
               
               
                   
                 AHI48827 12 AHI48825 2 AHI48824 3 AHI48821 12 AHI48819 
                   
                   
                   
                   
               
               
                   
                 15 AHI48818 3 AHI48815 12 AHI48812 15 AHI48810 16 
                   
                   
                   
                   
               
               
                   
                 AHI48808 15 AHI48806 15 AHI48804 15 AHI48803 6 
                   
                   
                   
                   
               
               
                   
                 AHI48802 1 AHI48801 6 AHI48800 6 AHI48799 2 AHI48798 69 
                   
                   
                   
                   
               
               
                   
                 AHI48797 25 AHI48796 47 AHI48795 53 AHI48794 67 
                   
                   
                   
                   
               
               
                   
                 AHI48793 28 AHI48792 61 AHI48791 75 AHI48790 4 
                   
                   
                   
                   
               
               
                   
                 AHI48789 61 AHI48788 5 AHI48787 36 AHI48786 15 
                   
                   
                   
                   
               
               
                   
                 AHI48785 14 AHI48784 59 AHI48783 25 AHI48782 25 
                   
                   
                   
                   
               
               
                   
                 AHI48781 22 AHI48780 4 AHI48779 30 AHI48778 35 
                   
                   
                   
                   
               
               
                   
                 AHI48777 63 AHI48776 133 AHI48775 16 AHI48774 18 
                   
                   
                   
                   
               
               
                   
                 AHI48773 9 AHI48772 18 AHI48771 10 AHI48770 11 
                   
                   
                   
                   
               
               
                   
                 AHI48769 100 AHI48768 148 AHI48767 100 AHI48766 143 
                   
                   
                   
                   
               
               
                   
                 AHI48765 100 AHI48764 148 AHI48763 100 AHI48762 145 
                   
                   
                   
                   
               
               
                   
                 AHI48761 100 AHI48760 144 AHI48759 100 AHI48758 142 
                   
                   
                   
                   
               
               
                   
                 AHI48757 100 AHI48756 148 AHI48755 100 AHI48754 146 
                   
                   
                   
                   
               
               
                   
                 AHI48753 4 AHI48752 23 AHI48751 4 AHI48750 23 AHI48749 
                   
                   
                   
                   
               
               
                   
                 10 AHI48748 42 AHI48747 14 AHI48746 22 AHI48745 10 
                   
                   
                   
                   
               
               
                   
                 AHI48744 52 AHI48743 4 AHI48742 12 AHI48741 4 AHI48740 
                   
                   
                   
                   
               
               
                   
                 23 AHI48739 13 AHI48738 12 AHI48737 13 AHI48736 16 
                   
                   
                   
                   
               
               
                   
                 AHI48735 7 AHI48734 12 AHI48733 13 AHI48732 12 
                   
                   
                   
                   
               
               
                   
                 AHI48731 7 AHI48730 12 AHI48729 7 AHI48728 12 AHI48727 
                   
                   
                   
                   
               
               
                   
                 13 AHI48726 12 AHI48725 3 AHI48724 12 AHI48723 1 
                   
                   
                   
                   
               
               
                   
                 AHI48722 12 AHI48721 1 AHI48720 12 AHI48718 16 
                   
                   
                   
                   
               
               
                   
                 AHI48717 3 AHI48714 12 AHI48711 13 AHI48710 12 
                   
                   
                   
                   
               
               
                   
                 AHI48709 8 AHI48708 3 AHI48705 12 AHI48702 13 AHI48701 
                   
                   
                   
                   
               
               
                   
                 12 AHI48699 8 AHI48698 3 AHI48695 12 AHI48692 13 
                   
                   
                   
                   
               
               
                   
                 AHI48691 14 AHI48689 15 AHI48688 3 AHI48685 12 
                   
                   
                   
                   
               
               
                   
                 AHI48682 13 AHI48681 12 AHI48679 15 AHI48678 3 
                   
                   
                   
                   
               
               
                   
                 AHI48675 12 AHI48672 13 AHI48671 12 AHI48669 8 
                   
                   
                   
                   
               
               
                   
                 AHI48668 3 AHI48665 12 AH148662 13 AHI48661 14 
                   
                   
                   
                   
               
               
                   
                 AHI48659 16 AHI48658 3 AHI48655 12 AH148652 13 
                   
                   
                   
                   
               
               
                   
                 AHI48651 3 AHI48649 15 AHI48648 3 AHI48647 15 AHI48646 
                   
                   
                   
                   
               
               
                   
                 3 AHI48644 15 AHI48643 3 AHI48641 15 AHI48640 3 
                   
                   
                   
                   
               
               
                   
                 AHI48638 15 AHI48637 3 AHI48635 15 AHI48634 3 AHI48633 
                   
                   
                   
                   
               
               
                   
                 15 AHI48632 3 AHI48629 12 AHI48626 13 AHI48625 72 
                   
                   
                   
                   
               
               
                   
                 AHI48624 134 AHI48623 15 AHI48622 3 AHI48619 12 
                   
                   
                   
                   
               
               
                   
                 AHI48616 13 AHI48615 75 AHI48614 137 AHI48612 15 
                   
                   
                   
                   
               
               
                   
                 AHI48611 3 AHI48608 12 AHI48605 13 AHI48604 100 
                   
                   
                   
                   
               
               
                   
                 AHI48603 162 AHI48601 15 AHI48600 3 AHI48597 12 
                   
                   
                   
                   
               
               
                   
                 AHI48594 13 AHI48593 98 AHI48592 160 AHI48590 15 
                   
                   
                   
                   
               
               
                   
                 AHI48589 3 AHI48586 12 AHI48583 13 AHI48582 98 
                   
                   
                   
                   
               
               
                   
                 AHI48581 160 AHI48579 15 AHI48578 3 AHI48575 12 
                   
                   
                   
                   
               
               
                   
                 AHI48572 13 AHI48571 100 AHI48570 162 AHI48568 15 
                   
                   
                   
                   
               
               
                   
                 AHI48567 3 AHI48564 12 AHI48561 13 AHI48560 100 
                   
                   
                   
                   
               
               
                   
                 AHI48559 162 AHI48557 15 AHI48556 3 AHI48553 12 
                   
                   
                   
                   
               
               
                   
                 AHI48550 13 AHI48549 100 AHI48548 162 AHI48546 15 
                   
                   
                   
                   
               
               
                   
                 AHI48545 3 AHI48542 12 AHI48539 13 AHI48538 100 
                   
                   
                   
                   
               
               
                   
                 AHI48537 162 AHI48535 15 AHI48534 3 AHI48531 12 
                   
                   
                   
                   
               
               
                   
                 AHI48528 13 AHI48527 100 AHI48526 162 AHI48524 15 
                   
                   
                   
                   
               
               
                   
                 AHI48523 3 AHI48520 12 AHI48517 13 AHI48516 100 
                   
                   
                   
                   
               
               
                   
                 AHI48515 162 AHL31380 1 AHL31379 12 AHL31378 1 
                   
                   
                   
                   
               
               
                   
                 AHL31377 9 AHL31376 1 AHL31375 9 AHL31374 1 
                   
                   
                   
                   
               
               
                   
                 AHL31373 9 AHL31372 1 AHL31371 9 AHL31370 1 
                   
                   
                   
                   
               
               
                   
                 AHL31369 9 AHL31368 1 AHL31367 1 AHL31366 1 
                   
                   
                   
                   
               
               
                   
                 AHL31365 1 AHL31364 1 AHL31363 1 AHL31362 1 
                   
                   
                   
                   
               
               
                   
                 AHL31361 1 AHL31360 1 AHL31359 1 AHL18098 13 
                   
                   
                   
                   
               
               
                   
                 AHL18097 3 AHL18094 12 AHL18091 160 AHL18090 13 
                   
                   
                   
                   
               
               
                   
                 AHL18089 101 K9N5Q8 13 K9N7A1 3 AHC74105 15 
                   
                   
                   
                   
               
               
                   
                 AHC74104 3 AHC74101 12 AHC74098 13 AHC74097 62 
                   
                   
                   
                   
               
               
                   
                 AHC74096 101 AHC74095 15 AHC74094 3 AHC74091 12 
                   
                   
                   
                   
               
               
                   
                 AHC74088 13 AHC74087 62 AHC74086 101 AHC74084 12 
                   
                   
                   
                   
               
               
                   
                 AHC74082 2 AHC74081 2 AHC74080 5 AHB33333 15 
                   
                   
                   
                   
               
               
                   
                 AHB33332 3 AHB33329 12 AHB33326 13 AHB33325 99 
                   
                   
                   
                   
               
               
                   
                 AHB33324 161 4MOD_B 6 4MOD_A 6 K9N4V7 21 4L3N_B 1 
                   
                   
                   
                   
               
               
                   
                 4L3N_A 1 AGV08597 8 AGV08596 100 AGV08595 143 
                   
                   
                   
                   
               
               
                   
                 AGV08594 6 AGV08593 15 AGV08580 15 AGV08579 3 
                   
                   
                   
                   
               
               
                   
                 AGV08576 12 AGV08573 13 AGV08572 100 AGV08571 162 
                   
                   
                   
                   
               
               
                   
                 AGV08570 18 AGV08569 1 AGV08568 12 AGV08567 43 
                   
                   
                   
                   
               
               
                   
                 AGV08565 15 AGV08564 3 AGV08561 12 AGV08558 13 
                   
                   
                   
                   
               
               
                   
                 AGV08557 100 AGV08556 162 AGV08555 53 AGV08553 15 
                   
                   
                   
                   
               
               
                   
                 AGV08552 3 AGV08549 12 AGV08546 13 AGV08545 100 
                   
                   
                   
                   
               
               
                   
                 AGV08544 162 AGV08542 15 AGV08541 3 AGV08538 12 
                   
                   
                   
                   
               
               
                   
                 AGV08535 13 AGV08534 100 AGV08533 162 AGV08531 15 
                   
                   
                   
                   
               
               
                   
                 AGV08530 3 AGV08527 12 AGV08524 14 AGV08523 52 
                   
                   
                   
                   
               
               
                   
                 AGV08522 42 AGV08520 1 AGV08519 6 AGV08517 15 
                   
                   
                   
                   
               
               
                   
                 AGV08516 3 AGV08515 25 AGV08514 12 AGV08512 15 
                   
                   
                   
                   
               
               
                   
                 AGV08511 3 AGV08508 12 AGV08505 13 AGV08504 44 
                   
                   
                   
                   
               
               
                   
                 AGV08502 15 AGV08501 3 AGV08499 15 AGV08498 3 
                   
                   
                   
                   
               
               
                   
                 AGV08495 12 AGV08492 13 AGV08491 100 AGV08490 162 
                   
                   
                   
                   
               
               
                   
                 AGV08489 23 AGV08487 16 AGV08486 3 AGV08483 12 
                   
                   
                   
                   
               
               
                   
                 AGV08480 13 AGV08479 100 AGV08478 162 AGV08477 7 
                   
                   
                   
                   
               
               
                   
                 AGV08476 12 AGV08474 15 AGV08473 3 AGV08470 12 
                   
                   
                   
                   
               
               
                   
                 AGV08467 13 AGV08466 100 AGV08465 162 AGV08464 25 
                   
                   
                   
                   
               
               
                   
                 AGV08462 15 AGV08461 3 AGV08458 12 AGV08455 13 
                   
                   
                   
                   
               
               
                   
                 AGV08454 100 AGV08453 162 AGV08451 15 AGV08450 3 
                   
                   
                   
                   
               
               
                   
                 AGV08447 12 AGV08444 13 AGV08443 100 AGV08442 162 
                   
                   
                   
                   
               
               
                   
                 AGV08441 7 AGV08438 13 AGV08437 44 AGV08436 27 
                   
                   
                   
                   
               
               
                   
                 AGV08435 17 AGV08433 8 AGV08432 3 AGV08429 12 
                   
                   
                   
                   
               
               
                   
                 AGV08426 13 AGV08425 12 AGV08423 12 AGV08420 6 
                   
                   
                   
                   
               
               
                   
                 AGV08418 15 AGV08417 3 AGV08405 2 AGV08404 16 
                   
                   
                   
                   
               
               
                   
                 AGV08403 25 AGV08402 37 AGV08401 25 AGV08400 1 
                   
                   
                   
                   
               
               
                   
                 AGV08399 9 AGV08397 15 AGV08396 3 AGV08393 12 
                   
                   
                   
                   
               
               
                   
                 AGV08390 13 AGV08389 97 AGV08388 159 4L72_B 1 4L72_A 
                   
                   
                   
                   
               
               
                   
                 12 4KR0_B 7 4KR0_A 12 4KQZ_B 7 4KQZ_A 7 K9N643 12 
                   
                   
                   
                   
               
               
                   
                 AGN72646 15 AGN72645 3 AGN72642 12 AGN72639 162 
                   
                   
                   
                   
               
               
                   
                 AGN72638 100 AGN70980 15 AGN70979 3 AGN70976 12 
                   
                   
                   
                   
               
               
                   
                 AGN70973 13 AGN70972 100 AGN70971 162 AGN70970 15 
                   
                   
                   
                   
               
               
                   
                 AGN70968 3 AGN70965 12 AGN70962 13 AGN70961 100 
                   
                   
                   
                   
               
               
                   
                 AGN70960 162 AGN70958 15 AGN70957 3 AGN70954 12 
                   
                   
                   
                   
               
               
                   
                 AGN70951 13 AGN70950 100 AGN70949 162 AGN70936 15 
                   
                   
                   
                   
               
               
                   
                 AGN70935 3 AGN70932 12 AGN70929 13 AGN70928 100 
                   
                   
                   
                   
               
               
                   
                 AGN70927 162 AGN52936 13 
                   
                   
                   
                   
               
               
                 2014  
                 AHY21476 15 AHY21475 3 AHY21472 12 AHY21469 13  
                 12 
                 2.6 
                 1.4 
                 prev p &gt; .50 
               
               
                   
                 AHY21468 160 AHY21467 98 AHX71953 16 AHX71952 3 
                   
                   
                   
                   
               
               
                   
                 AHX71949 12 AHX71946 13 AHX71945 100 AHX71944 162 
               
               
                   
               
            
           
         
       
     
     Example 12 
     Conserved MERS CoV Sequences 
     The following sequences are provided as examples of sequences conserved in isolates of MERS CoV across time and in various regions. Each sequence is provided below with a listing of the accession number and position in the publicly-disclosed sequence wherein the peptide sequence begins. 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 22) 
               
               
                   
                 KNKMRHK  
               
            
           
         
       
     
     2012 
     AGV08591 position 247, AGV08415 position 247, AGV08386 position 247, YP_007188586 position 247, AFY13314 position 247, AGG22549 position 247, AGH58724 position 247, AFS88943 position 247. 
     2013 
     AGV08580 position 247, AGV08565 position 247, AGV08553 position 247, AGV08542 position 247, AGV08531 position 247, AGV08517 position 247, AGV08512 position 247, AGV08502 position 247, AGV08499 position 247, AGV08487 position 247, AGV08474 position 247, AGV08462 position 247, AGV08451 position 247, AGV08433 position 247, AGV08418 position 247, AGV08397 position 247, K9N4V7 position 247, AGN72646 position 247, AGN70980 position 247, AGN70970 position 247, AGN70958 position 247, AGN70936 position 247. 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 18) 
               
               
                   
                 KAAVHKWK  
               
            
           
         
       
     
     2012 
     AGV08583 position 1568, AGV08582 position 1568, AGV08407 position 1568, AGV08406 position 1568, AGV08378 position 1568, AGV08377 position 1568, YP 007188577 position 1568, YP 007188578 position 1568, AFY13306 position 1568, AFY13305 position 1568, AGG22541 position 1568, AGG22540 position 1568. 
     2013 
     K9N638 position 1568, K9N7C7 position 1568, AGV08596 position 1568, AGV08595 position 1568, AGV08572 position 1568, AGV08571 position 1568, AGV08557 position 1568, AGV08556 position 1568, AGV08555 position 155, AGV08545 position 1568, AGV08544 position 1568, AGV08534 position 1568, AGV08533 position 1568, AGV08522 position 241, AGV08491 position 1568, AGV08490 position 1568, AGV08479 position 1568, AGV08478 position 1568, AGV08466 position 1568, AGV08465 position 1568, AGV08454 position 1568, AGV08453 position 1568, AGV08443 position 1568, AGV08442 position 1568, AGV08436 position 525, AGV08402 position 156, AGV08389 position 1568, AGV08388 position 1568, AGN72639 position 1568, AGN72638 position 1568, AGN70972 position 1568, AGN70971 position 1568, AGN70961 position 1568, AGN70960 position 1568, AGN70950 position 1568, AGN70949 position 1568, AGN70928 position 1568, AGN70927 position 1568. 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 23) 
               
               
                   
                 KRSHSPTK  
               
            
           
         
       
     
     2012 
     AGV08587 position 23, AGV08411 position 23, AGV08382 position 23, YP 007188582 position 23, AFY13310 position 23, AGG22545 position 23, AFS88939 position 23. 
     2013 
     AGV08576 position 23, AGV08561 position 23, AGV08549 position 23, AGV08538 position 23, AGV08527 position 23, AGV08508 position 23, AGV08495 position 23, AGV08483 position 23, AGV08470 position 23, AGV08458 position 23, AGV08447 position 23, AGV08441 position 23, AGV08429 position 23, AGV08423 position 23, AGV08393 position 23, K9N643 position 23, AGN72642 position 23, AGN70976 position 23, AGN70965 position 23, AGN70954 position 23, AGN70932 position 23. 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 5) 
               
               
                   
                 KARKRSHSPTK  
               
            
           
         
       
     
     2012 
     AGV08587 position 20, AGV08411 position 20, AGV08382 position 20, YP 007188582 position 20, AFY13310 position 20, AGG22545 position 20, AFS88939 position 20. 
     2013 AGV08576 position 20, AGV08561 position 20, AGV08549 position 20, AGV08538 position 20, AGV08527 position 20, AGV08508 position 20, AGV08495 position 20, AGV08483 position 20, AGV08470 position 20, AGV08458 position 20, AGV08447 position 20, AGV08441 position 20, AGV08429 position 20, AGV08423 position 20, AGV08393 position 20, K9N643 position 20, AGN72642 position 20, AGN70976 position 20, AGN70965 position 20, AGN70954 position 20, AGN70932 position 20. 
     Conserved sequences are useful as targets for control of replication and lethality in vaccines. A vaccine combining conserved sequences or employing individual sequences or conserved sequences as vaccine targets may be used to prevent or control MERS CoV infection and outbreaks. 
     Example 13 
     MERS COV Replikin Concentrations Compared Between Time Periods 
     Gene and amino acid sequences publicly available for isolates of MERS CoV were queried at www.pubmed.com. Replikin concentrations were determined and numbers of isolates with Replikin concentrations greater than 4.0 were compared to numbers of isolates with Replikin concentrations 4.0 or less. A percentage was determined for each time period. 
     Table 1 provides the data from April 2012. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 Date of Publication 
                   
                   
                   
               
               
                 of Specimen 
                 Replikin 
                 Gene 
                 Accession 
               
               
                 YYYMMDD 
                 Concentration 
                 Segment 
                 Number 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 20120400 
                 1  
                 S 
                 AGH58717 
               
               
                 20120400 
                 1.4 
                 M 
                 AGH58718 
               
               
                 20120400 
                 4.9 
                 NS3c 
                 K9N643 
               
               
                 20120400 
                 2.2 
                 ORF1a 
                 AGH58715 
               
               
                 20120400 
                 2.3 
                 ORF1ab 
                 AGH58716 
               
               
                 20120400 
                 2.3 
                 ORF1a 
                 K9N638 
               
               
                 20120400 
                 3.6 
                 N 
                 AGH58724 
               
               
                 20120400 
                 4.9 
                 ORF4b 
                 AGH58721 
               
               
                 20120400 
                 2.2 
                 ORF1a 
                 AGH58715 
               
               
                 20120400 
                 2.2 
                 ORF1a 
                 AGH58715 
               
               
                 20120400 
                 2.3 
                 ORF1ab 
                 AGH58716 
               
               
                 20120400 
                 1 
                 S 
                 AGH58717 
               
               
                 20120400 
                 1.4 
                 M 
                 AGH58718 
               
               
                 20120400 
                 4.9 
                 ORF4b 
                 AGH58721 
               
               
                   
               
            
           
         
       
     
     The number of isolates from April 2012 was 15. The number of isolates with a Replikin concentration of greater than 4.0 was 3. The percentage of isolates with a Replikin concentration of greater than 4.0 is 20%. 
     Table 2 provides the data from May through June 2012. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
               
                 Date of Publication 
                   
                   
                   
               
               
                 of Specimen 
                 Replikin 
                   
                   
               
               
                 YYYMMDD 
                 Concentration 
                 Gene Segment 
                 Accession Number 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 20120513 
                 3.6 
                 N 
                 AFS88943 
               
               
                 20120513 
                 3.6 
                 N 
                 AFS88943 
               
               
                 20120513 
                 3.6 
                 N 
                 AGV08415 
               
               
                 20120612 
                 1.4 
                 M 
                 AGV08414 
               
               
                 20120613 
                 1 
                 S 
                 AFS88936 
               
               
                 20120613 
                 6.1 
                 ORF4b 
                 AFS88939 
               
               
                 20120613 
                 2.3 
                 ORF1ab 
                 AFS88944 
               
               
                 20120613 
                 2.3 
                 ORF1a 
                 AFV09327 
               
               
                   
               
            
           
         
       
     
     The number of isolates from May through June 2012 was 8. The number of isolates with a Replikin concentration of greater than 4.0 was 1. The percentage of isolates with a Replikin concentration of greater than 4.0 is 12.50%. 
     Table 3 provides the data from September 2012. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 3 
               
               
                   
               
               
                 Date of Publication 
                   
                   
                   
               
               
                 of Specimen 
                 Replikin 
                   
                   
               
               
                 YYYMMDD 
                 Concentration 
                 Gene Segment 
                 Accession Number 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 20120900 
                 2.3 
                 ORF1ab 
                 K9N7C7 
               
               
                 20120900 
                 1.4 
                 M 
                 K9N7A1 
               
               
                 20120900 
                 1 
                 NS3C 
                 K9N5Q8 
               
               
                 20120900 
                 5.1 
                 N 
                 K9N4V7 
               
               
                 20120911 
                 2.3 
                 ORF1a 
                 AFY13305 
               
               
                 20120911 
                 2.3 
                 ORF1b 
                 AFY13306 
               
               
                 20120911 
                 1 
                 S 
                 AFY13307 
               
               
                 20120911 
                 4.9 
                 NS3C 
                 AFY13310 
               
               
                 20120911 
                 1.4 
                 M 
                 AFY13313 
               
               
                 20120911 
                 5.1 
                 N 
                 AFY13314 
               
               
                 20120911 
                 1.4 
                 M 
                 AFY13313 
               
               
                 20120911 
                 1 
                 S 
                 AFY13307 
               
               
                 20120911 
                 2.3 
                 ORF1a 
                 AFY13305 
               
               
                 20120911 
                 2.3 
                 ORF1b 
                 AFY13306 
               
               
                 20120911 
                 4.9 
                 NS3C 
                 AFY13310 
               
               
                 20120911 
                 5.1 
                 N 
                 AFY13314 
               
               
                 20120919 
                 2.3 
                 ORF1ab 
                 AGG22540 
               
               
                 20120919 
                 2.3 
                 ORF1a 
                 AGG22541 
               
               
                 20120919 
                 1 
                 S 
                 AGG22542 
               
               
                 20120919 
                 4.9 
                 ORF4b 
                 AGG22545 
               
               
                 20120919 
                 1.4 
                 M 
                 AGG22548 
               
               
                 20120919 
                 5.1 
                 N 
                 AGG22549 
               
               
                 20120919 
                 1.4 
                 M 
                 AGG22548 
               
               
                 20120919 
                 1 
                 S 
                 AGG22542 
               
               
                 20120919 
                 2.3 
                 ORF1ab 
                 AGG22540 
               
               
                 20120919 
                 4.9 
                 ORF4b 
                 AGG22545 
               
               
                 20120919 
                 5.1 
                 N 
                 AGG22549 
               
               
                   
               
            
           
         
       
     
     The number of isolates from September 2012 was 30. The number of isolates with a Replikin concentration of greater than 4.0 was 9. The percentage of isolates with a Replikin concentration of greater than 4.0 is 30%. 
     Table 4 provides the data from October 2012. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 4 
               
               
                   
               
               
                 Date of Publication 
                   
                   
                   
               
               
                 of Specimen 
                 Replikin 
                   
                   
               
               
                 YYYMMDD 
                 Concentration 
                 Gene Segment 
                 Accession Number 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 20121016 
                 1.4 
                 M 
                 AFS88942 
               
               
                 20120000 
                 4.4 
                 ORF1ab 
                 AGO05997 
               
               
                 20120000 
                 2.7 
                 ORF1ab 
                 AGO06000 
               
               
                 20120000 
                 3.3 
                 ORF1ab 
                 AGO06001 
               
               
                 20120000 
                 4.3 
                 S 
                 AGO06003 
               
               
                 20121023 
                 2.3 
                 ORF1ab 
                 AGV08377 
               
               
                 20121023 
                 2.3 
                 ORF1a 
                 AGV08378 
               
               
                 20121023 
                 1 
                 S 
                 AGV08379 
               
               
                 20121023 
                 7.6 
                 ORF4b 
                 AGV08382 
               
               
                 20121023 
                 1.4 
                 M 
                 AGV08385 
               
               
                 20121023 
                 3.6 
                 N 
                 AGV08386 
               
               
                 20121016 
                 1.4 
                 M 
                 AFS88942 
               
               
                 20120000 
                 4.4 
                 ORF1ab  
                 AGO05997 
               
               
                   
               
            
           
         
       
     
     The number of isolates from October 2012 was 11. The number of isolates with a Replikin concentration of greater than 4.0 was 3. The percentage of isolates with a Replikin concentration of greater than 4.0 is 27%. 
     Table 5 provides the data from February 2013. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 5 
               
               
                   
               
               
                 Date of Publication 
                   
                   
                   
               
               
                 of Specimen 
                 Replikin 
                   
                   
               
               
                 YYYMMDD 
                 Concentration 
                 Gene Segment 
                 Accession Number 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 20130000 
                 2.3 
                 ORF1ab 
                 AGN72639 
               
               
                 20130000 
                 4.9 
                 ORF4b 
                 AGN72642 
               
               
                 20130000 
                 2.3 
                 ORF1ab 
                 AGN72639 
               
               
                 20130000 
                 2.3 
                 ORF1ab 
                 AGN72638 
               
               
                 20130000 
                 1 
                   
                 AGN52936 
               
               
                 20130000 
                 1 
                 S 
                 AGN52936 
               
               
                 20130205 
                 2.2 
                 ORF1ab 
                 AGV08388 
               
               
                 20130205 
                 2.2 
                 ORF1a 
                 AGV08389 
               
               
                 20130205 
                 1 
                 S 
                 AGV08390 
               
               
                 20130205 
                 2.2 
                 ORF1ab 
                 AGV08388 
               
               
                 20130205 
                 2.2 
                 ORF1a 
                 AGV08389 
               
               
                 20130205 
                 1 
                 S 
                 AGV08390 
               
               
                 20130205 
                 4.9 
                 ORF4b 
                 AGV08393 
               
               
                 20130205 
                 1.4 
                 M 
                 AGV08396 
               
               
                 20130205 
                 3.6 
                 N 
                 AGV08397 
               
               
                 20130000 
                 2.3 
                 ORF1ab 
                 AGN72639 
               
               
                   
               
            
           
         
       
     
     The number of isolates from February 2013 was 15. The number of isolates with a Replikin concentration of greater than 4.0 was 2. The percentage of isolates with a Replikin concentration of greater than 4.0 is 13.30%. 
     Table 6 provides the data from April 2013. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 6 
               
               
                   
               
               
                 Date of Publication 
                   
                   
                   
               
               
                 of Specimen 
                 Replikin 
                   
                   
               
               
                 YYYMMDD 
                 Concentration 
                 Gene Segment 
                 Accession Number 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 20130421 
                 1 
                 S 
                 AGN70951 
               
               
                 20130421 
                 2.3 
                 ORF1a 
                 AGN70950 
               
               
                 20130421 
                 2.3 
                 ORF1ab 
                 AGN70949 
               
               
                 20130421 
                 4.9 
                 ORF4b 
                 AGN70954 
               
               
                 20130421 
                 1.4 
                 M 
                 AGN70957 
               
               
                 20130421 
                 3.6 
                 N 
                 AGN70958 
               
               
                 20130422 
                 2.3 
                 ORF1ab 
                 AGN70971 
               
               
                 20130422 
                 2.3 
                 ORF1a 
                 AGN70972 
               
               
                 20130422 
                 1 
                 S 
                 AGN70973 
               
               
                 20130422 
                 4.9 
                 ORF4b 
                 AGN70976 
               
               
                 20130422 
                 1.4 
                 M 
                 AGN70979 
               
               
                 20130422 
                 3.6 
                 N 
                 AGN70980 
               
               
                 20130421 
                 1 
                 S 
                 AGN70951 
               
               
                   
               
            
           
         
       
     
     The number of isolates from April 2013 was 11. The number of isolates with a Replikin concentration of greater than 4.0 was 2. The percentage of isolates with a Replikin concentration of greater than 4.0 is 18.2%. 
     Table 7 provides the data from May 1, 2013. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 7 
               
               
                   
               
               
                 Date of Publication 
                   
                   
                   
               
               
                 of Specimen 
                 Replikin 
                   
                   
               
               
                 YYYMMDD 
                 Concentration 
                 Gene Segment 
                 Accession Number 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 20130501 
                 2.3 
                 ORF1ab 
                 AGN70927 
               
               
                 20130501 
                 2.3 
                 ORF1a 
                 AGN70928 
               
               
                 20130501 
                 1 
                 S 
                 AGN70929 
               
               
                 20130501 
                 4.9 
                 ORF4b 
                 AGN70932 
               
               
                 20130501 
                 1.4 
                 M 
                 AGN70935 
               
               
                 20130501 
                 3.6 
                 N 
                 AGN70936 
               
               
                 20130501 
                 2.5 
                 ORF1ab 
                 AGV08404 
               
               
                 20130501 
                 1.5 
                 S 
                 AGV08420 
               
               
                 20130501 
                 4.9 
                 ORF4b 
                 AGV08423 
               
               
                 20130501 
                 2.8 
                 ORF1ab 
                 AGV08425 
               
               
                 20130501 
                 1 
                 S 
                 AGV08426 
               
               
                 20130501 
                 4.9 
                 ORF4b 
                 AGV08429 
               
               
                 20130501 
                 1.4 
                 M 
                 AGV08432 
               
               
                 20130501 
                 2.9 
                 N 
                 AGV08433 
               
               
                 20130501 
                 1.9 
                 ORF1ab 
                 AGV08437 
               
               
                 20130501 
                 1 
                 S 
                 AGV08438 
               
               
                 20130501 
                 10.1 
                 ORF4b 
                 AGV08441 
               
               
                 20130501 
                 1.4 
                 M 
                 AGV08501 
               
               
                 20130501 
                 3.6 
                 N 
                 AGV08502 
               
               
                   
               
            
           
         
       
     
     The number of isolates from May 1, 2013 was 19. The number of isolates with a Replikin concentration of greater than 4.0 was 4. The percentage of isolates with a Replikin concentration of greater than 4.0 is 21.1%. 
     Table 8 provides the data from May 2-30, 2013. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 8 
               
               
                   
               
               
                 Date of Publication 
                   
                   
                   
               
               
                 of Specimen 
                 Replikin 
                   
                   
               
               
                 YYYMMDD 
                 Concentration 
                 Gene Segment 
                 Accession Number 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 20130502 
                 2.9 
                 ORF1ab 
                 AGV08435 
               
               
                 20130502 
                 3.1 
                 ORF1ab 
                 AGV08399 
               
               
                 20130502 
                 0.3 
                 S 
                 AGV08400 
               
               
                 20130503 
                 3.9 
                 ORF1ab 
                 AGV08464 
               
               
                 20130503 
                 3.9 
                 ORF1ab 
                 AGV08464 
               
               
                 20130507 
                 2.9 
                 ORF1a 
                 AGV08402 
               
               
                 20130507 
                 2.3 
                 ORF1ab 
                 AGV08442 
               
               
                 20130507 
                 2.3 
                 ORF1a 
                 AGV08443 
               
               
                 20130507 
                 1 
                 S 
                 AGV08444 
               
               
                 20130507 
                 4.9 
                 ORF4b 
                 AGV08447 
               
               
                 20130507 
                 1.4 
                 M 
                 AGV08450 
               
               
                 20130507 
                 3.6 
                 N 
                 AGV08451 
               
               
                 20130508 
                 1.1 
                 ORF1ab 
                 AGV08401 
               
               
                 20130509 
                 4 
                 ORF1ab 
                 AGV08403 
               
               
                 20130509 
                 0.7 
                 ORF1ab 
                 AGV08405 
               
               
                 20130509 
                 2 
                 M 
                 AGV08417 
               
               
                 20130509 
                 3.6 
                 N 
                 AGV08418 
               
               
                 20130509 
                 3.9 
                 ORF1ab 
                 AGV08436 
               
               
                 20130509 
                 3 
                 ORF1ab 
                 AGV08476 
               
               
                 20130509 
                 0.7 
                 S 
                 AGV08477 
               
               
                 20130509 
                 2.4 
                 ORF1ab 
                 AGV08489 
               
               
                 20130509 
                 2.3 
                 ORF1ab 
                 AGN70960 
               
               
                 20130509 
                 2.3 
                 ORF1a 
                 AGN70961 
               
               
                 20130509 
                 1 
                 S 
                 AGN70962 
               
               
                 20130509 
                 4.9 
                 ORF4b 
                 AGN70965 
               
               
                 20130509 
                 1.4 
                 M 
                 AGN70968 
               
               
                 20130513 
                 2.3 
                 ORF1ab 
                 AGV08465 
               
               
                 20130513 
                 2.3 
                 ORF1a 
                 AGV08466 
               
               
                 20130513 
                 1 
                 S 
                 AGV08467 
               
               
                 20130513 
                 4.9 
                 ORF4b 
                 AGV08470 
               
               
                 20130513 
                 1.4 
                 M 
                 AGV08473 
               
               
                 20130513 
                 3.6 
                 N 
                 AGV08474 
               
               
                 20130515 
                 2.8 
                 S 
                 4KQZ_B 
               
               
                 20130515 
                 2.8 
                 S 
                 4KR0_B 
               
               
                 20130523 
                 2.3 
                 ORF1ab 
                 AGV08478 
               
               
                 20130523 
                 2.3 
                 Orf1a 
                 AGV08479 
               
               
                 20130523 
                 1 
                 S 
                 AGV08480 
               
               
                 20130523 
                 4.9 
                 ORF4b 
                 AGV08483 
               
               
                 20130523 
                 1.4 
                 M 
                 AGV08486 
               
               
                 20130523 
                 3.9 
                 N 
                 AGV08487 
               
               
                 20130530 
                 2.3 
                 ORF1ab 
                 AGV08490 
               
               
                 20130530 
                 2.3 
                 ORF1a 
                 AGV08491 
               
               
                 20130530 
                 1 
                 S 
                 AGV08492 
               
               
                 20130530 
                 4.9 
                 ORF4b 
                 AGV08495 
               
               
                 20130530 
                 1.4 
                 M 
                 AGV08498 
               
               
                 20130530 
                 3.6 
                 N 
                 AGV08499 
               
               
                   
               
            
           
         
       
     
     The number of isolates from May 2-30, 2013 was 48. The number of isolates with a Replikin concentration of greater than 4.0 was 7. The percentage of isolates with a Replikin concentration of greater than 4.0 is 14.6%. 
     Table 9 provides the data from June 2013. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 9 
               
               
                   
               
               
                 Date of Publication 
                   
                   
                   
               
               
                 of Specimen 
                 Replikin 
                   
                   
               
               
                 YYYMMDD 
                 Concentration 
                 Gene Segment 
                 Accession Number 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 20130604 
                 2.3 
                 ORF1a 
                 AGV08454 
               
               
                 20130604 
                 1 
                 S 
                 AGV08455 
               
               
                 20130604 
                 4.9 
                 ORF4b 
                 AGV08458 
               
               
                 20130604 
                 1.4 
                 M 
                 AGV08461 
               
               
                 20130604 
                 3.6 
                 N 
                 AGV08462 
               
               
                 20130606 
                 0.5 
                 N3 
                 4L3N_A 
               
               
                 20130619 
                 2.3 
                 ORF1ab 
                 AGV08406 
               
               
                 20130619 
                 2.3 
                 ORF1a 
                 AGV08407 
               
               
                 20130619 
                 1 
                 S 
                 AGV08408 
               
               
                 20130619 
                 7.6 
                 ORF4b 
                 AGV08411 
               
               
                   
               
            
           
         
       
     
     The number of isolates from June 2013 was 10. The number of isolates with a Replikin concentration of greater than 4.0 was 2. The percentage of isolates with a Replikin concentration of greater than 4.0 is 20%. 
     Example 14 
     Analysis of AHY22523 
     Accession number AHY22523 was analyzed. The following sequences were identified and with further analysis were determined to be conserved among camels and humans. AHY22523 is entitled “Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia Direct Submission.” 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 26) 
               
               
                   
                 HVERKDVPYPK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 13) 
               
               
                   
                 KGDSCSSNCKH 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 27) 
               
               
                   
                 KGMQLLHTK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 19) 
               
               
                   
                 KLNPSEDFIKH 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 18) 
               
               
                   
                 KAAVHKWK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 6) 
               
               
                   
                 KHVEVFTDGK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 20) 
               
               
                   
                 KFCDHMVK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 28) 
               
               
                   
                 KEGSSVTLKH 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 14) 
               
               
                   
                 HARLKGGLILK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 11) 
               
               
                   
                 KFYQHVINGCK 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 12) 
               
               
                   
                 KQVHQVQLTDK. 
               
            
           
         
       
     
     A synthetic Replikin vaccine containing an approximately equal-parts-by-weight mixture of the eleven MERS CoV Replikin peptides was designed for use against relatively lethal isolates of MERS CoV. The vaccine was engineered from sequences confirmed to be conserved among camels and humans across time. Conservation was particularly noted in the key amino acid residues of the Replikin sequence, namely, lysine and histidine amino acid residues. The vaccine was engineered to inhibit the lethality of relatively lethal strains of MERS CoV. 
     The vaccine may be administered to an animal or human susceptible, exposed to, or suffering from infection of MERS CoV. The blocking mechanism of the vaccine provides a therapeutic and prophylactic effect. The immune response generated by the vaccine provides a prophylactic effect. Infection and replication are blocked by targeting these sequences. 
     Example 15 
     Analysis of all Isolates of MERS-CoV 
     All isolates of the virus genes reported to date in at the NCBI PubMed database from both humans and camels was undertaken and revealed specific shared Replikin structures which were not just homologous but identical in the virus genes infecting both human and camel hosts, conserved since 2012 in cases from all countries affected. A Replikins Synthetic Vaccine based on these identical shared structures was produced. This vaccine is an inhibitor of the progression of MERS-CoV. The Anti-MERS-CoV vaccine was prepared for testing in humans and camels. 
     Camels are understood to be a reservoir and source of human MERS infection mostly because of the detection in camels of antibodies that react with MERS (1,2). The present quantitative gene specific structural findings support this understanding. 
     SARS, a coronavirus related to MERS, produced approximately 8000 human cases and 800 deaths in 2003. MERS has a higher mortality rate than SARS. MERS started in 2012 with an approximate mortality rate of 40%, and now, perhaps with better care, is still approximately 27%. The duration of the outbreak is already greater. This is in contrast to SARS, who&#39;s abrupt rise and rapid decline in gene Replikin concentration signaled in 2002 that the 2003 outbreak would occur but would be over soon (and it was within one year). The outbreak of MERS in 2012, the increased Replikin concentrations reported in October 2013, and the upsurge in MERS clinical outbreaks in 2014 now indicate a more prolonged course and a greater risk of pandemic. In agreement with these differences, the highest individual gene Replikin concentrations observed to date in MERS is double that in SARS. 
     The Replikins Synthetic Anti-MERS Vaccine is unique because:
         1. Prediction: The vaccine is based on gene technology which correctly predicts, one to two years in advance, a coming breakthrough or pandemic, as established retrospectively one year before the SARS outbreak of 2003; and prospectively i) in June 2013 for the current MERS outbreaks, ii) in 2006 for the 2007 H5N1 lethal outbreak in Indonesia, and iii) in 2008 for the 2009 H1N1 pandemic.   2. The concentration of gene Replikin sequences (Replikin Count=Number of Replikins per 100 amino acids) relates quantitatively to rapid replication of the pathogen, and to morbidity and mortality, whether the pathogen is a virus, bacterium, or cancer cell.   3. The prediction identifies the specific gene Replikin structures involved in the rapid replication and outbreaks of the attacking pathogen.   4. The prediction also defines the geographic areas that have the highest pathogen Replikins activity, from which global spread initiates, and to which public health and vaccine containment efforts can be directed (as with Indonesia for H5N1 in 2006, and Saudi Arabia for the current MERS outbreaks).   5. The specific gene Replikin structures identified are the basis of the synthetic vaccine designed;   6. Such Replikin vaccines have been produced freeze-dried by solid phase synthesis in 7 days, can be produced in unlimited quantities before the outbreak peaks, have been shipped without need of refrigeration, and have been found effective against Taura Syndrome virus in shrimp and influenza H5N1 virus in chickens.   7. The discovery of identical MERS gene Replikin structures in both infected humans and in camels provides a surprising defined parallel host relationship for vaccine testing.       

     Replikin sequences, therefore, provide a complete defense system, not previously available, beginning with prediction pre-outbreak, based on detection and specific identification of the quantitative gene changes associated with rapid replication. These gene changes can be addressed in advance of, or after, outbreaks occur, providing time to possibly prevent pandemics rather than just react to them. 
     While it would have been preferable to begin testing the Replikins vaccine when the upturn in gene Replikin Count was noted in October 2013, there still may be time to reduce or interrupt the present progression of MERS-CoV. 
     1. CIDRAP NEWS. Reports: Mers-CoV found in Saudi&#39;s patient&#39;s camel. Nov. 11, 2013.
 
2. Memish, Ziad. Promed Mail. Animal reservoirs, camels. http://www.isid.org Nov. 12, 2013.
 
3. Bogoch S and Bogoch ES. Nature Precedings doi: 10.1038/npre.2012.6952.1 Bogoch Replikins Pandemic Prevention: Increase of Strain-Specific Influenza Genomic Replikin Counts, Having Predicted Outbreaks and their Location Seven Times Consecutively, Up to Two Years in Advance, Provides Time for Prevention of Pandemics.
 
     Example 16 
     October 2013 Identification of Increasing Replikin Concentration in MERS-CoV 
     In October 2013, the applicants identified an increase in Replikin concentration in isolates of MERS-CoV. 
     The BBC reported the inventor&#39;s analysis that Middle East Respiratory Syndrome corona virus (MERS) has undergone several fluctuations in the virus&#39; ability to replicate and could be rising again. The MERS Replikin concentration peaked in virulence and infectivity in September of 2012 with a count of 27.7% and dropped sharply to a low of 14.3% by February of 2013. The count began to rise again and as of June 2013 (the last month for which data were available at the time of analysis in October 2013) had reached 20%. “MERS Replikin Count Increases; Updated Data Needed,” BBC News Blog, Monday, Oct. 21, 2013, Michelle Blowers 3:56 p.m.