Patent Publication Number: US-2010122586-A1

Title: Automatic analyzer and dispensing method

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is a continuation of PCT international application Ser. No. PCT/JP2008/063211 filed on Jul. 23, 2008 which designates the United States, incorporated herein by reference, and which claims the benefit of priority from Japanese Patent Application No. 2007-191305, filed on Jul. 23, 2007, incorporated herein by reference. 
    
    
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The present invention relates to an automatic analyzer and a dispensing method. 
     2. Description of the Related Art 
     Conventionally, when dispensing a specimen, such as blood (whole blood) for analyzing hemoglobin A1c (HbA1c), which is a component of red blood cells, a dispensing device of an automatic analyzer used for dispensing a specimen or a reagent detects the liquid level of the blood contained in a specimen vessel and dispenses the blood with the tip end of a dispensing probe inserted to a depth in consideration of the settling of the red blood cells. The blood (whole blood) is one example of such specimen, in which a concentration gradient occurs in a vertical direction due to the settling of a component in accordance with the passage of time after the specimen is collected (For example, see Japanese Laid-open Patent Publication No. 2000-121650). 
     SUMMARY OF THE INVENTION 
     An automatic analyzer according to an aspect of the invention that stirs a plurality of different liquids to induce a reaction and measures an optical characteristic of a reaction liquid, thereby analyzing the reaction liquid, includes a stirring unit that includes a vibrator that is arranged on a vessel that contains a specimen including a sedimented component; and an electrode that is arranged on a transfer path for transferring a rack, on which the vessel is arranged, to a dispensing position and feeds electric power to the vibrator, wherein the stirring unit feeds the electric power from the electrode to the vibrator while the rack is being transferred to the dispensing position along the transfer path and stirs the specimen including the sedimented component contained in the vessel. 
     An automatic analyzer according to another aspect of the present invention that stirs a plurality of different liquids to induce a reaction and measures an optical characteristic of a reaction liquid, thereby analyzing the reaction liquid, includes a stirring unit that includes a vibrator that is arranged on a rack on which a vessel that contains a specimen including a sedimented component is arranged; and an electrode that is arranged on a transfer path for transferring the rack to a dispensing position and feeds electric power to the vibrator, wherein the stirring unit feeds the electric power from the electrode to the vibrator while the rack is being transferred to the dispensing position along the transfer path and stirs the specimen including the sedimented component contained in the vessel. 
     A dispensing method according to still another aspect of the invention for dispensing a specimen that includes a sedimented component, includes a stirring step for stirring the specimen that includes the sedimented component before dispensing. 
     The above and other features, advantages and technical and industrial significance of this invention will be better understood by reading the following detailed description of presently preferred embodiments of the invention, when considered in connection with the accompanying drawings. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a schematic configuration diagram that illustrates an automatic analyzer of the present invention; 
         FIG. 2  is a block diagram that illustrates the configuration of the automatic analyzer; 
         FIG. 3  is a plain view that illustrates the arrangement of a specimen stirring unit by enlarging a specimen-vessel transferring device of the automatic analyzer; 
         FIG. 4  is a perspective view that illustrates the arrangement of feed electrodes arranged along a transfer path of the specimen-vessel transferring device and receive electrodes arranged on a rack; 
         FIG. 5  is a cross-sectional view, which is sectioned in a width direction, of the rack that holds a specimen vessel; 
         FIG. 6  is a flowchart that illustrates a dispensing method of the present invention; 
         FIG. 7  is a plain view that explains the arrangement of the specimen stirring unit and corresponds to  FIG. 3 ; 
         FIG. 8  is a perspective view that explains the configuration of a fixed stirring unit of the specimen stirring unit and corresponds to  FIG. 4 ; 
         FIG. 9  is a perspective view that illustrates an example where a plurality of feed electrodes is arranged on the transfer path illustrated in  FIG. 4 ; 
         FIG. 10  is a plain view that explains a different arrangement of the specimen stirring unit and corresponds to  FIG. 3 ; 
         FIG. 11  is an enlarged plain view that explains the configuration of a movable stirring unit of the specimen stirring unit; 
         FIG. 12  is a cross-sectional view, which is sectioned in a width direction, of a rack that includes a fixed specimen stirring unit and holds the specimen vessel; 
         FIG. 13  is a cross-sectional view, which is sectioned in a longitudinal direction, of a rack that includes a fixed specimen stirring unit and holds a specimen vessel; 
         FIG. 14  illustrates a modified example 1 of the specimen stirring unit and is a cross-sectional view, which is sectioned in a width direction, of a rack that holds the specimen vessel; 
         FIG. 15  illustrates the modified example 1 of the specimen stirring unit and is a cross-sectional view, which is sectioned in a longitudinal direction, of a rack that holds the specimen vessel; 
         FIG. 16  illustrates a further modification of the modified example 1 and is a cross-sectional view, which is sectioned in a width direction, of a rack that holds the specimen vessel; 
         FIG. 17  illustrates a modified example 2 of the specimen stirring unit and is a cross-sectional view, which is sectioned in a width direction, of a rack that holds the specimen vessel; 
         FIG. 18  illustrates the modified example 2 of the specimen stirring unit and is a cross-sectional view, which is sectioned in a longitudinal direction, of a rack that holds the specimen vessel; and 
         FIG. 19  illustrates a further modification of the modified example 2 and is a cross-sectional view, which is sectioned in a width direction, of a rack that holds the specimen vessel. 
     
    
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     A detailed explanation will be given of an embodiment of an automatic analyzer and a dispensing method of the present invention with reference to the drawings.  FIG. 1  is a schematic configuration diagram that illustrates an automatic analyzer of the present invention.  FIG. 2  is a block diagram that illustrates the configuration of the automatic analyzer.  FIG. 3  is a plain view that illustrates the arrangement of a specimen stirring unit by enlarging a specimen-vessel transferring device of the automatic analyzer. 
     As illustrated in  FIGS. 1 and 2 , an automatic analyzer  1  includes reagent tables  2 ,  3 , a reaction table  4 , a specimen-vessel transferring device  8 , a specimen dispensing device  11 , a specimen stirring unit  12  (see  FIG. 3 ), an analysis optical system  13 , a cleaning device  14 , a stirrer  15 , and a control unit  17 . 
     As illustrated in  FIG. 1 , the reagent tables  2 ,  3  hold a plurality of reagent vessels  2   a,    3   a,  respectively, arranged in a circumferential direction and are rotated by a driving means so as to transfer the reagent vessels  2   a,    3   a  in the circumferential direction. 
     As illustrated in  FIG. 1 , the reaction table  4  has a plurality of reaction vessels  5  arranged in a circumferential direction and is rotated clockwise or counterclockwise by a driving means different from the driving means of the reagent tables  2 ,  3  so as to transfer the reaction vessels  5 . The reaction table  4  rotates (one revolution subtracted by one reaction vessel)/4 for one cycle in a clockwise direction and rotates (one revolution subtracted by one reaction vessel) for four cycles, for example. 
     The reaction vessel  5  is a vessel whose capacity is very small, from several nL to several hundred and a transparent material is used through which more than 80% of light contained in the analysis light emitted by a light emitting unit  13   a  of the analysis optical system  13  is transmitted. For example, glass that includes heat-resistant glass or synthetic resin such as cyclic olefin or polystyrene may be used. The reaction vessel  5  is a square cylindrical cuvette with a square horizontal cross sectional area, in which a liquid is retained, and an opening at the top. Reagents are dispensed into the reaction vessels  5  from the reagent vessels  2   a,    3   a  of the reagent tables  2 ,  3  by reagent dispensing devices  6 ,  7  arranged near the reaction table  4 . 
     The reagent dispensing devices  6 ,  7  have probes  6   b,    7   b,  respectively, which dispense reagents, attached to arms  6   a,    7   a  that are rotated in a horizontal plane in the directions indicated by arrows and include a cleaning means that cleans the probes  6   b,    7   b  by using cleaning water. 
     As illustrated in  FIG. 3 , the specimen-vessel transferring device  8  is a transferring means, such as a belt conveyor, that transfers a plurality of arranged racks  9  one by one in the direction indicated by the arrow or in the opposite direction. The specimen-vessel transferring device  8  includes a transverse transfer path  8   a  for transferring the racks  9  in a transverse direction and a longitudinal transfer path  8   b  for transferring the racks  9  in a longitudinal direction. The specimen-vessel transferring device  8  transfers the racks  9  fed to a set position Ps (see  FIG. 3 ) of the transverse transfer path  8   a  by moving them step by step in the directions indicated by the arrows along the transverse transfer path  8   a  and the longitudinal transfer path  8   b.  The rack  9  holds a plurality of specimen vessels  10  that contain specimens in a recessed portion  9   a  (see  FIG. 5 ). A plurality of receive electrodes  9   c  is arranged on a lower portion of a side wall  9   b  in a longitudinal direction. 
     Each time the step-moving of the racks  9  transferred by the specimen-vessel transferring device  8  is stopped, the specimen dispensing device  11  dispenses a specimen into the reaction vessel  5  from each of the specimen vessels  10  located at a dispensing position Pp on the transfer path of the specimen-vessel transferring device  8 . The specimen dispensing device  11  includes a drive arm  11   a  and a probe  11   b  that are rotated in a horizontal direction and a liquid-level detecting means as well as a cleaning means (not illustrated) that cleans the probe  11   b  using cleaning water. 
     The specimen stirring unit  12  is a stirring means that stirs a specimen that includes a sedimented component. As illustrate in  FIG. 3 , the specimen stirring unit  12  is arranged along the one longitudinal transfer path  8   b  of the specimen-vessel transferring device  8  that transfers the racks  9  from the set position Ps of the racks  9  to the dispensing position Pp by step-moving them in a longitudinal direction. The specimen stirring unit  12  includes a feed electrode  12   a  and a vibrator  12   b  (see FIG.  5 ) arranged at the bottom of the specimen vessel  10 . 
     As illustrated in  FIG. 4 , the feed electrodes  12   a  are arranged on both sides of the longitudinal transfer path  8   b  of the specimen-vessel transferring device  8 . The vibrator  12   b  is driven by receiving drive electric power fed due to the contact between the receive electrode  9   c  and the feed electrode  12   a  via a feed electrode  9   d  arranged at the bottom of the recessed portion  9   a  of the rack  9 , as illustrated in  FIG. 5 , thereby stirring a specimen S that contains a sedimented component in the specimen vessel  10  without making contact. Two feed electrodes  12   a  may be arranged on one side of the longitudinal transfer path  8   b  instead of both sides thereof. 
     For example, a surface acoustic wave element with a plurality of comb-teeth electrodes (IDT) formed on one surface of a piezoelectric substrate made of lithium niobate (LiNbO3), or the like, is used for the vibrator  12   b,  and the vibrator  12   b  stirs a liquid contained in the specimen vessel  10  by using a surface acoustic wave or bulk wave. The vibrator  12   b  is arranged at the bottom that becomes a horizontal flat surface via a curved area of the lower portion of the specimen vessel  10 . In the cross-sectional views of the rack  9  used in the following drawings including  FIG. 5 , hatching is omitted to place priority on viewability of the drawings. 
     The analysis optical system  13  emits analysis light to analyze the liquid contained in the reaction vessel  5  where the reagent and the specimen are reacted. The analysis optical system  13  includes, as illustrated in  FIG. 1 , the light emitting unit  13   a,  a light splitting unit  13   b,  and a light receiving unit  13   c.  The analysis light emitted by the light emitting unit  13   a  is transmitted through the liquid contained in the reaction vessel  5  and is received by the light receiving unit  13   c  located at a position opposed to the light splitting unit  13   b.  The light receiving unit  13   c  is connected to the control unit  17  and outputs a light intensity signal of the received analysis light to the control unit  17 . 
     After sucking up and discharging the liquid contained in the reaction vessel  5  by using a nozzle  14   a,  the cleaning device  14  repeats an operation of injecting and sucking up a cleaning liquid, such as detergent or cleaning water, via the nozzle  14   a  a plurality of times, thereby cleaning the inside of the reaction vessel  5  for which the optical measurement is finished by the analysis optical system  13 . 
     A microcomputer or the like is used for the control unit  17 , for example. As illustrated in  FIGS. 1 and 2 , the control unit  17  is connected to each component of the automatic analyzer  1  so as to control the operation of each component and analyzes constituent concentrations, and the like, of a specimen on the basis of the absorbance of the liquid contained in the reaction vessel  5  in accordance with the intensity of light output from the light emitting unit  13   a  and the intensity of light received by the light receiving unit  13   c.  Furthermore, the control unit  17  determines the position of the specimen vessel  10 , for which the stirring is required, along the transfer path of the specimen-vessel transferring device  8  by using information about the specimen vessel  10 , for which the stirring is required, input from a host computer and position information of the rack  9  input from the specimen-vessel transferring device  8 . The control unit  17  controls the specimen stirring unit  12  to feed drive electric power to the feed electrode  12   a  that corresponds to the specimen vessel  10  for which the stirring is required. 
     The control unit  17  causes an analysis operation to be performed while controlling the operation of each component of the automatic analyzer  1  in accordance with an analysis instruction input from an input unit  18 , such as a keyboard, and displays various types of information, and the like, in accordance with a display instruction input from the input unit  18  in addition to an analysis result or warning information on a display unit  19 , such as a display panel. Besides this, the control unit  17  detects abnormalities that include a contact failure of the vibrator  12   b,  or the like, on the basis of the reflection of the drive electric power from the vibrator  12   b  arranged at the bottom of the specimen vessel  10  and stores therein the number of times an abnormality is detected. The control unit  17  changes the settings of the dispensing operation relating to the specimen dispensing device  11  and the cleaning operation of the probe  11   b  when a conventional dispensing method for dispensing a usual specimen by deeply inserting the probe  11   b  into the specimen and a dispensing method of the present invention for stirring a specimen that contains a sedimented component before dispensing and inserting the probe  11   b  into a specimen to a shallow depth are used. 
     The stirrer  15  stirs the liquid contained in the reaction vessel  5  by using ultrasound that is sound generated by driving a surface acoustic wave element  15   c  attached to the reaction vessel  5  and has a frequency that exceeds an audible frequency. The stirrer  15  includes an electric-power transmitting member  15   a  that transmits electric power fed from a high-frequency alternating-current source of about several MHz to several hundred MHz to the surface acoustic wave element  15   c and an arrangement determining member  15   b  that adjusts the relative arrangement of the electric-power transmitting member  15   a  and an electric terminal in the circumferential direction and the radial direction of the reaction table  4 . 
     The automatic analyzer  1  that has the above-described configuration is operated under the control of the control unit  17 . The reagent dispensing devices  6 ,  7  sequentially dispense reagents from the reagent vessels  2   a,    3   a  into the plurality of reaction vessels  5  transferred by the rotating reaction table  4  in a circumferential direction. Specimens are sequentially dispensed by the specimen dispensing device  11  into the reaction vessels  5 , into which the reagents have been dispensed, from the plurality of specimen vessels  10  held by the rack  9 . 
     Each time the reaction table  4  is stopped, the reaction vessel  5  in which the reagent and the specimen have been dispensed is sequentially stirred by the stirrer  15 , whereby the reagent and the specimen are reacted, and when the reaction table  4  is rotated again, the reaction vessel  5  passes by the analysis optical system  13 . At that time, the optical measurement is performed on the reaction liquid contained in the reaction vessel  5  by the light receiving unit  13   c,  and the constituent concentration, or the like, is analyzed by the control unit  17 . The reaction vessel  5 , for which the optical measurement of the reaction liquid is finished, is cleaned by the cleaning device  14  and then is used for analysis of a specimen again. 
     The automatic analyzer  1  includes the specimen stirring unit  12  arranged on the transfer path of the specimen-vessel transferring device  8 . The plurality of specimen vessels  10  held by the rack  9  transferred along the transfer path of the specimen-vessel transferring device  8  has the receive electrodes  9   c  arranged on the lower portion of the side wall  9   b  sequentially brought into contact with the feed electrodes  12   a  in accordance with the step-moving of the rack  9 . As a result, when the specimen vessel  10  that contains a specimen including a sedimented component reaches the specimen stirring unit  12 , the vibrator  12   b  receives the drive electric power sent under the control of the control unit  17  via the feed electrode  9   d,  and the specimen that includes the sedimented component is uniformly stirred by the sound flow caused by the ultrasound generated by the vibrator  12   b  due to the drive electric power. 
     An explanation will be given below of a specimen dispensing method performed under the control of the control unit  17  with reference to the flowchart illustrated in  FIG. 6 . 
     First, the control unit  17  determines whether the specimen vessel  10  that contains the specimen including the sedimented component and for which the stirring is required has reaches the specimen stirring unit  12  (step S 100 ). The position of the specimen vessel  10  is detected on the basis of information, which is input from the host computer to the control unit  17 , about the specimen vessel  10  that contains the specimen including the sedimented component as the stepping position of the specimen vessel  10  along the transfer path of the specimen-vessel transferring device  8 , and it is determined whether the detected stepping position of the specimen vessel  10  is the position of the specimen stirring unit  12 . 
     If the detected stepping position of the specimen vessel  10  is not the position of the specimen stirring unit  12  (step S 100 , No), the control unit  17  goes back to step S 100  and determines whether the specimen vessel  10  has reached the specimen stirring unit  12 . Conversely, if the detected stepping position of the specimen vessel  10  is the position of the specimen stirring unit  12  (step S 100 , Yes), the control unit  17  starts to stir the specimen that includes the sedimented component contained in the specimen vessel  10  (step S 102 ). At that time, the stirring is performed such that, after it is detected that the specimen vessel  10  has reached the specimen stirring unit  12  and after the step-moving by the specimen-vessel transferring device  8  has stopped, the control unit  17  controls an electric-power feed unit to feed drive electric power to the feed electrode  12   a  that corresponds to the specimen vessel  10  for which the stirring is required. 
     Next, the control unit  17  determines whether an abnormality is detected after the stirring has started (step S 104 ). If an abnormality, such as a contact failure between the receive electrode  9   c  and the feed electrode  12   a,  is not detected after the stirring has started (step S 104 , No), the specimen that includes the sedimented component contained in the specimen vessel  10  is uniformly stirred by the specimen stirring unit  12 . Therefore, the control unit  17  stops the drive electric power from being fed to the feed electrode  12   a  and terminates the stirring of the specimen (step S 106 ). 
     Afterward, the control unit  17  causes the specimen vessel  10  in which the specimen has been uniformly stirred to move step by step to the dispensing position (step S 108 ). Then, the control unit  17  causes the specimen dispensing device  11  to dispense the uniformly stirred specimen into the reaction vessel  5  from the specimen vessel  10  (step S 110 ). At that time, because the specimen has been uniformly stirred in advance, the specimen dispensing device  11  can always dispense the specimen at a constant concentration simply by inserting the lower end of the probe  11   b  into the specimen to a certain level. 
     Next, the control unit  17  causes the cleaning means to clean the probe lib of the specimen dispensing device  11  (step S 112 ). At that time, because the lower end of the probe  11   b  is only slightly inserted into the specimen, a small amount of cleaning water is required to be used by the cleaning means for cleaning. Then, the control unit  17  determines whether the stirring of all of the specimen vessels  10  for which the stirring is required has finished on the basis of information, which is input from the host computer to the control unit  17 , about the specimen vessels  10  that contain specimens including sedimented components (step S 114 ). 
     If the stirring of all of the specimen vessels  10  has not finished (step S 114 , No), the control unit  17  goes back to step S 100 . If the stirring of all of the specimen vessels  10  has finished (step S 114 , Yes), the control unit  17  terminates the method of dispensing the specimens from the specimen vessels  10  that contain the specimens including the sedimented components. 
     Conversely, if an abnormality, such as a contact failure between the receive electrode  9   c  and the feed electrode  12   a,  is detected after the stirring has started (step S 104 , Yes), the control unit  17  determines whether the abnormality is detected for the first time (step S 116 ). If the number of times the abnormality is detected is the first time (step S 116 , Yes), the control unit  17  executes to stop the feeding of the drive electric power to the feed electrode  12   a  and stop the specimen vessel  10  again to the stepping position by the specimen-vessel transferring device  8  (step S 118 ). Afterward, the control unit  17  goes back to step S 102  and resumes the stirring. At that time, the control unit  17  notifies the host computer of an indication that the abnormality, such as a contact failure, is detected. 
     The contact failure between the feed electrode  9   d  and the vibrator  12   b  can be resolved by reinstalling the specimen vessel  10  in the recessed portion  9   a.  Furthermore, the detected abnormality can be, other than the contact failure between the receive electrode  9   c  and the feed electrode  12   a,  for example, a failure of the vibrator  12   b,  and in this case, the specimen vessel  10  is replaced. As explained in a modified example 1, if the vibrator  12   b  is arranged on the side of the rack  9 , the position of the recessed portion  9   a  where the specimen vessel  10  that is a stirring target is arranged is changed. 
     Conversely, if the number of times the abnormality is detected is not the first time (step S 116 , No), the control unit  17  stops the drive electric power from being fed to the feed electrode  12   a  and terminates the stirring of the specimen (step S 120 ). Afterward, the control unit  17  causes the specimen vessel  10  to move step by step to the dispensing position (step S 122 ). The control unit  17  then changes the settings of the dispensing operation of the specimen dispensing device  11  (step S 124 ). 
     Next, the control unit  17  causes the specimen dispensing device  11  to dispense the specimen from the specimen vessel  10  into the reaction vessel  5  (step S 126 ). At that time, the specimen dispensing device  11 , under the control of the control unit  17 , dispenses the specimen with the probe lib deeply inserted into the specimen in the specimen vessel  10  in the same manner as the case where the specimen is dispensed in a state where the sedimented component in the specimen contained in the specimen vessel  10  has settled. 
     Subsequently, the control unit  17  changes the settings of the cleaning operation of the probe  11   b  (step S 128 ). The control unit  17  then cleans the probe  11   b,  by which the specimen has been dispensed, in accordance with the changed cleaning operation (step S 130 ). At that time, the cleaning means, which cleans the probe  11   b,  sufficiently cleans a part of the probe lib deeply inserted into the specimen in the same manner as in the case of cleaning the probe  11   b  that has dispensed a specimen in a state where the sedimented component in the specimen contained in the specimen vessel  10  has settled. Afterward, the control unit  17  skips to step S 114  and performs the steps after step S 114 . 
     As is clear from the above explanation, as illustrated in  FIG. 3 , the specimen that includes the sedimented component contained in the specimen vessel  10  is uniformly stirred by the sound flow caused by the ultrasound generated by the vibrator  12   b  in the specimen stirring unit  12  before being dispensed at the dispensing position Pp on the transfer path of the specimen-vessel transferring device  8 . Therefore, even if the specimen includes a sedimented component, the specimen dispensing device  11  can dispense the uniformly stirred specimen into the reaction vessel  5  from the specimen vessel  10  simply by always inserting the tip end of the probe  11   b  into the specimen to a certain level in the same manner as for the other usual specimens. As a result, the specimen dispensing device  11  does not need to deeply insert the tip end of the probe  11   b  into the specimen even if the specimen includes a sedimented component, the same cleaning as in the case where a usual specimen is dispensed is only required to be performed, and the same dispensing operation and the same cleaning operation are only required to be performed always; therefore, the control of the operation becomes easier. 
     The specimen stirring unit  12  can be arranged at any position between the set position Ps and the dispensing position Pp if a time period from when the rack  9 , on which the specimen vessel  10  that contains a specimen including a sedimented component is set, is arranged to when the arranged rack  9  is transferred to the dispensing position Pp by the specimen-vessel transferring device  8  does not affect an analysis result due to the settling of a sedimented component (for example, 15 to 30 minutes for whole blood components). For example, as illustrated in  FIG. 7 , the specimen stirring unit  12  is arranged at least at one position from a second stirring unit P 2  to a ninth stirring unit P 9 . 
     These stirring units are the same fixed stirring units as the specimen stirring unit  12  illustrated in  FIG. 3  and are arranged along the longitudinal transfer path  8   b  of the specimen-vessel transferring device  8 . For example, the second stirring unit P 2  has the feed electrode  12   a  arranged at the bottom of the longitudinal transfer path  8   b,  as illustrated in  FIG. 8  ( FIG. 9 ). The rack  9  that holds the specimen vessels  10  has the plurality of receive electrodes  9   c  arranged at the bottom that corresponds to the feed electrode  12   a.  As illustrated in  FIG. 9 , it is possible that the plurality of feed electrodes  12   a  is arranged on both sides of the longitudinal transfer path  8   b  and the feed electrode  12   a  to which the electric power is fed is changed so that a specimen in the specimen vessel  10  held at a predetermined position of the rack  9  is stirred. In this case, it is possible that the plurality of feed electrodes  12   a  is arranged at the bottom of the longitudinal transfer path  8   b.  Furthermore, the vibrator  12   b  can be arranged on the side surface near the bottom surface if the vibrator  12   b  is arranged on the bottom of the specimen vessel  10 . 
     Moreover, if the stirring is also performed on the transverse transfer path  8   a,  as illustrated in  FIG. 10 , a first stirring unit P 1  and a fifth stirring unit P 5  are arranged on the transverse transfer path  8   a.  For example, the first stirring unit P 1  is a movable stirring unit that is arranged on the lower portion of the transverse transfer path  8   a  of the specimen-vessel transferring device  8  and, as illustrated in  FIG. 11 , has the two feed electrodes  12   a  arranged on the top surface of a slider  16   b  that slides along a rail  16   a  of a linear guide  16 , and the fifth stirring unit P 5  is also a movable stirring unit. In this case, the rack  9  has the feed electrode  12   a  arranged on its bottom surface. 
     With the above-described configuration, in the rack  9  transferred by the specimen-vessel transferring device  8 , as illustrated in  FIGS. 12 and 13 , the receive electrode  9 c arranged on the bottom surface is in contact with the feed electrode  12   a  via the feed electrode  9   d  and the vibrator  12   b  receives the drive electric power. As a result, a specimen S that includes a sedimented component contained in the specimen vessel  10  held by the rack  9  is uniformly stirred by the sound flow caused by the ultrasound generated by the vibrator  12   b  without making contact. 
     Thus, the specimen dispensing device  11  can always dispense a specimen with a certain concentration simply by inserting the end of the probe  11   b  into the specimen to a certain level in the same manner as for a usual liquid sample. Furthermore, because the specimen stirring unit  12  uses the surface acoustic wave element as the vibrator  12   b,  it is easier to arrange it along the specimen-vessel transferring device  8  compared to arranging a mechanical stirring means such as a stirring bar. Therefore, if the feed electrode  12   a  of the specimen stirring unit  12  can be arranged, there is an advantage in that the specimen dispensing device  11  can be easily arranged in the automatic analyzer  1  without making major structural modifications. 
     The specimen dispensing device  11  may, after dispensing a plasma component of blood contained in the specimen vessel  10  in the ninth stirring unit P 9 , stir the blood contained in the specimen vessel  10  and dispense the uniformly mixed whole blood. In this manner, it is possible to dispense the blood contained in the specimen vessel  10  into a plurality of vessels in accordance with different examination purposes without dividing one blood into a plurality of vessels for different examination purposes in advance. 
     If the abnormality is detected for a second time, it is often the case that a satisfactory result cannot be obtained even if the specimen dispensed into the reaction vessel  5  is analyzed. Therefore, the steps after step S 120  can be omitted. Furthermore, step S 130  can be performed at the same time as step S 126 . 
     Modified Example 1 
     As illustrated in  FIGS. 14 and 15 , instead of having the vibrator  12   b  arranged on the specimen vessel  10 , the specimen stirring unit  12  may have the vibrator  12   b  arranged at the bottom of the recessed portion  9   a  formed on the rack  9  so that the ultrasound generated by the vibrator  12   b  is propagated to the specimen S that includes a sedimented component contained in the specimen vessel  10  via an acoustic matching layer Lao made of oil, water, gel, or the like. As illustrated in  FIG. 16 , the rack  9  may have the receive electrodes  9   c  arranged on the lower portion of the side wall  9   b  so that the electric power is fed from the plurality of feed electrodes  12   a  arranged on both sides of the longitudinal transfer path  8   b  of the specimen-vessel transferring device  8  at a predetermined interval in the conveying direction of the rack  9 . If the drive frequency of the vibrator  12   b  is low, the acoustic matching layer Lao is not necessary. 
     Modified Example 2 
     Furthermore, the specimen stirring unit  12  may use a thickness longitudinal vibrator as the vibrator  12   b  instead of the surface acoustic wave element. In this case, because the vibrator  12   b  that uses a thickness longitudinal vibrator has a large amplitude of vibration, as illustrated in  FIGS. 17 and 18 , protruding portions  9   e  that are supporting points are arranged on the upper portion of the recessed portion  9   a  that holds the specimen vessel  10 , and an elastic member  9   f  that receives vibration applied by the vibrator  12   b  to the specimen vessel  10  is arranged at a position opposed to the vibrator  12   b.  With such a configuration, when the vibration is applied from the vibrator  12   b  to the specimen vessel  10  held in the recessed portion  9   a,  the lower portion of the specimen vessel  10  vibrates in a horizontal direction as indicated by the arrow in  FIG. 18  with the protruding portions  9   e  as the supporting points, whereby the contained specimen S that includes the sedimented component can be uniformly stirred. 
     The specimen stirring unit  12  can use a magnetostrictive vibrator, or the like, as the vibrator  12   b  in addition to an electrostrictive vibrator that includes the surface acoustic wave element or the thickness longitudinal vibrator described above. 
     Moreover, as illustrated in  FIG. 19 , the rack  9  may have the receive electrodes  9   c  arranged on the lower portion of the side wall  9   b  so that the electric power is fed from the plurality of feed electrodes  12   a  arranged on both sides of the longitudinal transfer path  8   b  of the specimen-vessel transferring device  8  at a predetermined interval in the conveying direction of the rack  9 . 
     The automatic analyzer and the dispensing method of the above-described embodiment are explained for the case where blood is dispensed as a specimen to analyze hemoglobin A1c that is a component of red blood cells. However, the automatic analyzer and the dispensing method of the present invention are not limited to a specimen such as blood if a specimen contains a sedimented component in which a concentration gradient occurs in a vertical direction due to the settling in accordance with the passage of time after the specimen is collected, and, for example, the automatic analyzer and the dispensing method of the present invention can be used for a specimen that contains body fluid such as spinal fluid, bile, sputum, or mucus, or a specimen such as river water, lake water, or ocean water, that contains a sedimented component such as suspended particulate organic matter. Moreover, the automatic analyzer and the dispensing method of the present invention can be used for control serum, or the like. 
     Meanwhile, the above-described embodiment is explained for the case of the stirring means that stirs the specimen by driving the vibrator arranged in the vessel that contains the specimen or the rack on which the vessel is arranged. However, if an arrangement space can be obtained, it is possible to use a stirring means that stirs the specimen by mechanically vibrating the vessel that contains the specimen or the rack on which the vessel is arranged. 
     Additional advantages and modifications will readily occur to those skilled in the art. Therefore, the invention in its broader aspects is not limited to the specific details and representative embodiments shown and described herein. Accordingly, various modifications may be made without departing from the spirit or scope of the general inventive concept as defined by the appended claims and their equivalents.