Patent Publication Number: US-2017369901-A1

Title: Methods and compositions to enhance activity of cry endotoxins

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application claims the benefit U.S. Provisional Application No. 61/713,844, filed Oct. 15, 2012, which is incorporated herein by reference in their entirety. 
    
    
     REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY 
     An official copy of the Sequence Listing submitted electronically as an ASCII formatted Sequence Listing with a file named “3113PCT_SequenceListing.txt,” created on Sep. 17, 2013, having a size of 158 kb and filed concurrently with the Specification is part of the Specification and is incorporated herein by reference as if set forth in its entirety. 
     FIELD OF THE INVENTION 
     The invention relates generally to plant molecular biology and plant pest control, and more particularly to compositions and methods for enhancing the activity of pesticidal polypeptides from  Bacillus  spp. and for protecting a plant from a plant pest, particularly an insect pest. 
     BACKGROUND OF THE INVENTION 
     Pests, such as insect pests, are a major factor in the loss of the world&#39;s agricultural crops. For example, corn rootworm and boll weevil damage can be economically devastating to agricultural producers. Insect pest-related agricultural crop loss from corn rootworm alone has reached one billion dollars a year. 
     Traditionally, the primary methods for controlling insect pests, such as corn rootworm, are crop rotation and application of broad-spectrum, synthetic, chemical pesticides. However, consumers and government regulators alike are becoming increasingly concerned with environmental hazards associated with producing and using chemical pesticides. Because of such concerns, regulators have banned or limited the use of some of the more hazardous chemical pesticides. Thus, there is substantial interest in developing alternatives to chemical pesticides that present a lower risk of pollution and environmental hazards and that provide a greater target specificity than is characteristic of chemical pesticides. 
     Certain species in the genus  Bacillus  have polypeptides that possess pesticidal activity against a broad range of insect pests including those in the orders Lepidoptera, Diptera, Coleoptera, Hemiptera and others. For example,  Bacillus thuringiensis  and  Bacillus popilliae  are among the most successful species discovered to date having pesticidal activity. Such pesticidal activity also has been attributed to strains of  Bacillus larvae, Bacillus lentimorbus, Bacillus sphaericus  and  Bacillus cereus.  See,  Biotechnology Handbook  2:  Bacillus  (Harwood ed., Plenum Press 1989); and Int&#39;l Patent Application Publication No. WO 96/10083. 
     Pesticidal polypeptides from  Bacillus  spp. include the crystal (Cry) endotoxins, cytolytic (Cyt) endotoxins, vegetative proteins (VIPs) and the like. See, e.g., Bravo et al. (2007)  Toxicon  49:423-435. The Cry endotoxins (also called δ-endotoxins) have been isolated from various strains of  B. thuringiensis.  A common characteristic of the Cry endotoxins is their expression during the stationary phase of growth, as they generally accumulate in a mother cell compartment to form a crystal inclusion that can account for 23-30% of the dry weight of sporulated cells. The Cry endotoxins initially are produced in an inactive protoxin form, which are proteolytically converted into an active endotoxin through the action of proteases in an insect&#39;s gut. Once active, the endotoxins bind to the gut epithelium and form cation-selective channels that cause cell lysis and subsequent death. See, Carroll et al. (1997)  J. Invertebr. Pathol.  70:41-49; Oppert (1999)  Arch. Insect Biochem. Phys.  42:1-12; and Rukmini et al. (2000)  Biochimie  82:109-116. 
     Although Cry endotoxins often are highly effective against insect pests, some insect pests are not affected by them or show low susceptibility. Likewise, some insect pests have developed resistance to the Cry endotoxins, which threatens their effectiveness. Methods to address these problems include enhancing or expanding Cry endotoxin activity by site-directed mutagenesis, by introducing cleavage sites in specific regions of the endotoxin or by deleting small fragments from the amino-terminus of the endotoxin. See, e.g., Abdullah &amp; Dean (2004)  Appl. Environ. Microbiol.  70:3769-3771; Pardo-López et al. (2009)  Peptides  30:589-595; Rajamohan et al. (1996)  Proc. Natl. Acad. Sci. USA  93:14338-14343; and Wu et al. (2000)  FEBS Lett.  473 227-232. These methods, however, can be time consuming and not certain to produce a desired result. 
     For the foregoing reasons, there is a need for compositions and methods to enhance the pesticidal activity of Cry endotoxins. 
     BRIEF SUMMARY OF THE INVENTION 
     Compositions and methods are provided for enhancing pesticidal activity of Cry endotoxins and for protecting a plant from plant pest such as insect pests. The compositions comprise chimeric pesticidal polypeptide-encoding nucleic acid molecules, variants and fragments thereof, as well as chimeric pesticidal polypeptides, active variants and fragments thereof. The chimeric pesticidal polypeptides of the invention comprise a solubility-enhancing polypeptide fused to a Cry endotoxin or biologically active fragment thereof. Also provided are expression cassettes or polynucleotide constructs comprising a nucleotide sequence encoding a chimeric pesticidal polypeptide of the invention, as well as bacteria, plants, plant organs, plants tissues, plant parts, plant cells and seeds comprising the expression cassette or polynucleotide nucleotide construct encoding the chimeric pesticidal polypeptide. Further provided are pesticidal compositions comprising at least one chimeric pesticidal polypeptide of the invention. 
     Methods are provided for enhancing the pesticidal activity of Cry endotoxins. The methods involve making a chimeric pesticidal polypeptide comprising the amino acid sequence of a solubility-enhancing polypeptide operably linked to an amino acid sequence of a Cry endotoxin or biologically active fragment thereof. Such chimeric pesticidal polypeptide can be produced by fusing amino acid sequence of the solubility-enhancing polypeptide to the amino acid sequence of a Cry endotoxin or biologically active fragment thereof. Alternatively, the methods involve making a polynucleotide construct comprising a nucleotide sequence encoding the chimeric pesticidal polypeptide and transforming an organism or non-human host cell of interest with the polynucleotide construct for expression of the chimeric pesticidal polypeptide. The nucleotide sequence encoding the chimeric pesticidal polypeptide can be produced by, for example, operably linking a nucleotide sequence encoding a solubility-enhancing polypeptide to a nucleotide sequence encoding a Cry endotoxin or biologically active fragment thereof. Typically, the polynucleotide construct further comprises a promoter that drives expression in the organism or host cell, wherein the promoter is operably linked to the nucleotide sequence encoding the chimeric pesticidal polypeptide. 
     Thus, methods are provided for producing a polynucleotide construct comprising a nucleotide sequence encoding a chimeric pesticidal polypeptide, which comprises an amino acid sequence of solubility-enhancing polypeptide fused to an amino acid sequence of a Cry endotoxin or biologically active fragment thereof. The methods involve operably linking a nucleotide sequence encoding solubility-enhancing polypeptide to a nucleotide sequence encoding a Cry endotoxin or biologically active fragment thereof. The polynucleotide construct may additional comprise an operably linked promoter for expression of the chimeric pesticidal polypeptide in a non-human host cell of interest, particularly a plant cell. Such a polynucleotide construct finds use, for example, in methods for expressing the chimeric pesticidal polypeptide in a plant transformed therewith. 
     The present invention further provides methods for making plants with enhanced resistance to at least one pest. The methods involved transforming a plant or at least one plant cell with a polynucleotide construct comprising a nucleotide sequence encoding a chimeric pesticidal polypeptide of the invention. Typically, the nucleotide sequence encoding a chimeric pesticidal polypeptide will be operably linked to a promoter that drives expression in a plant cell. The methods can further involve regenerating the plant or the at least one plant cell into a transformed plant, wherein the regenerated plant expresses the chimeric pesticidal polypeptide. Such a transformed plant comprises enhanced resistance to at least one plant pest, particularly an insect pest, when compared to the resistance of a control plant. 
     The methods also involve applying a composition such as a pesticidal composition comprising the chimeric pesticidal polypeptide or active variant or fragment thereof, to the environment of an insect pest, particularly on or in the vicinity of a plant by, for example, spraying, dusting, broadcasting or seed coating to protect the plant from the insect pest. 
     Further provided are transformed plants, plant cells and other host cells, and seeds comprising a nucleotide sequence encoding the chimeric pesticidal polypeptide of the invention. 
     The following embodiments are encompassed by the present invention: 
     1. A method of enhancing pesticidal activity of a Cry endotoxin, the method comprising operably linking a first amino acid sequence of a solubility-enhancing polypeptide to a second amino acid sequence of a Cry endotoxin, whereby a chimeric pesticidal polypeptide is produced, the chimeric pesticidal polypeptide comprising the first amino acid sequence operably linked to second amino acid sequence. 
     2. The method of embodiment 1, wherein the solubility-enhancing polypeptide is selected from the group consisting of a maltose-binding protein (MBP), a thioredoxin, a transcription elongation factor NusA, a glutathione-S-transferase (GST), a mistic, a small ubiquitin-related modifier (SUMO), a protein disulfide isomerase DsbC, and a thiol:disulfide interchange protein DsbD. 
     3. The method of embodiment 1 or 2, wherein the solubility-enhancing polypeptide is a MBP. 
     4. The method of embodiment 3, wherein the MBP is selected from the group consisting of MBPs having an accession number set forth in Table 1. 
     5. The method of embodiment 1 or 2, wherein the solubility-enhancing protein is NusA. 
     6. The method of embodiment 1 or 2, wherein the solubility-enhancing protein is thioredoxin. 
     7. The method of embodiment 1, wherein the solubility-enhancing polypeptide comprises an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 4, 6, 34, 35, and 36. 
     8. The method of any one of embodiments 1-7, wherein the Cry endotoxin is selected from the group consisting of Cry endotoxins set forth in Table 2. 
     9. The method of any one of embodiments 1-8, wherein the Cry endotoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NOS: 8, 10, 12, and 14. 
     10. The method of embodiment 1, wherein the chimeric pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 2, 16, 18, 21, 23, 25, 27, and 33. 
     11. The method of embodiment 1, wherein the chimeric pesticidal polypeptide is encoded by a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NO: 1, 15, 17, 20, 22, 24, 26, and 32. 
     12. The method of any one of embodiments 1-11, wherein the chimeric pesticidal polypeptide comprises a linker amino sequence between the first amino acid sequence and the second amino acid sequence. 
     13. The method of embodiment 1, wherein the linker amino acid sequence is selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 28, 29, 30, and 31. 
     14. The method of any one of embodiments 1-13, further comprising operably linking the linker amino acid sequence between the first amino acid sequence and the amino acid sequence, whereby the chimeric pesticidal polypeptide comprises in linear order the first amino acid sequence, the linker amino acid sequence, and the second amino acid sequence. 
     15. The method of any one of embodiments 1-15, wherein the chimeric pesticidal polypeptide comprises increased pesticidal activity against at least one pest, when compared to the pesticidal activity of the Cry endotoxin against the at least one pest. 
     16. The method of embodiment 15, wherein the at least one pest is an insect pest. 
     17. The method of embodiment 16, wherein the insect pest is an insect pest from the order Coleoptera or Lepidoptera. 
     18. The method of embodiment 16 or 17, wherein the insect pest is from the genus  Diabrotica.    
     19. The method of embodiment 16, wherein the insect pest is western corn rootworm. 
     20. The method of embodiment 16, wherein the insect pest is black cutworm. 
     21. A chimeric pesticidal polypeptide comprising a first amino acid sequence of a solubility-enhancing polypeptide operably linked to a second amino acid sequence of a Cry endotoxin. 
     22. The chimeric pesticidal polypeptide of embodiment 21, wherein the operably linked first and second amino sequences comprise an amino acid sequence selected from the group consisting of:
         (a) the amino acid sequence set forth in SEQ ID NO: 2, 16, 18, 21, 23, 25, 27 or 33;   (b) an amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO: 1, 15, 17, 20, 22, 24, 26 or 32; and   (c) an amino acid sequence comprising at least 80% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 2, 16, 18, 21, 23, 25, 27 or 33, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.       

     23. The chimeric pesticidal polypeptide of embodiment 21 or 22, wherein the solubility-enhancing polypeptide is a MBP. 
     24. The chimeric pesticidal polypeptide of embodiment 23, wherein the MBP is selected from the group consisting of MBPs having an accession number set forth in Table 1. 
     25. The chimeric pesticidal polypeptide of embodiment 21 or 22, wherein the solubility-enhancing protein is a NusA. 
     26. The chimeric pesticidal polypeptide of embodiment 21 or 22, wherein the solubility-enhancing protein is a thioredoxin. 
     27. The chimeric pesticidal polypeptide of embodiment 21, wherein the first amino acid sequence comprises an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 4, 6, 34, 35, and 36. 
     28. The chimeric pesticidal polypeptide of any one of embodiments 21-27, wherein the Cry endotoxin is selected from the group consisting of Cry endotoxins set forth in Table 2. 
     29. The chimeric pesticidal polypeptide of embodiment 21, wherein the Cry endotoxin comprises an amino acid sequence selected from the group consisting of SEQ ID NOS: 8, 10, 12, and 14. 
     30. The chimeric pesticidal polypeptide of any one of embodiments 21-29, wherein the chimeric pesticidal polypeptide further comprises a linker amino sequence operably linked between the first amino acid sequence and the second amino acid sequence. 
     31. The chimeric pesticidal polypeptide of embodiment 30, wherein the linker amino acid sequence is selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 28, 29, 30, and 31. 
     32. The chimeric pesticidal polypeptide of any one of embodiments 21-31, wherein the chimeric pesticidal polypeptide comprises increased pesticidal activity against at least one pest, when compared to the pesticidal activity of the Cry endotoxin against the at least one pest. 
     33. The chimeric pesticidal polypeptide of embodiment 32, wherein the at least one pest is an insect pest. 
     34. The chimeric pesticidal polypeptide of embodiment 33, wherein the insect pest is an insect pest from the order Coleoptera or Lepidoptera. 
     35. The chimeric pesticidal polypeptide of embodiment 33, wherein the insect pest is from the genus  Diabrotica.    
     36. The chimeric pesticidal polypeptide of embodiment 33, wherein the insect pest is western corn rootworm. 
     37. The chimeric pesticidal polypeptide of embodiment 33, wherein the insect pest is black cutworm. 
     38. A nucleic acid molecule comprising a nucleotide sequence encoding a chimeric pesticidal polypeptide, the chimeric pesticidal polypeptide comprising a first amino acid sequence of a solubility-enhancing polypeptide operably linked to a second amino acid sequence of a Cry endotoxin. 
     39. The nucleic acid molecule of embodiment 38, wherein the nucleotide sequence comprises a nucleotide sequence selected from the group consisting of:
         (a) the nucleotide sequence set forth in SEQ ID NO: 1, 15, 17, 20, 22, 24, 26 or 32;   (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 2, 16, 18, 21, 23, 25, 27 or 33;   (c) a nucleotide sequence comprising at least 80% nucleotide sequence identity to SEQ ID NO: 1, 15, 17, 20, 22, 24, 26 or 32, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; and   (e) a nucleotide sequence encoding an amino acid sequence comprising at least 80% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 2, 16, 18, 21, 23, 25, 27 or 33, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.       

     40. The nucleic acid molecule of embodiment 38 or 39, wherein the solubility-enhancing polypeptide is a MBP. 
     41. The nucleic acid molecule of embodiment 40, wherein the MBP is selected from the group consisting of MBPs having an accession number set forth in Table 1. 
     42. The nucleic acid molecule of embodiment 38 or 39, wherein the solubility-enhancing protein is a NusA. 
     43. The nucleic acid molecule of embodiment 38 or 39, wherein the solubility-enhancing protein is a thioredoxin. 
     44. The nucleic acid molecule of embodiment 38 or 39, wherein the first amino acid sequence comprises an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 4, 6, 34, 35 and 36. 
     45. The nucleic acid molecule of any one of embodiments 38-44, wherein the Cry endotoxin is selected from the group consisting of Cry endotoxins set forth in Table 2. 
     46. The nucleic acid molecule of any one of embodiments 38-44, wherein the second amino acid sequence encodes a Cry endotoxin selected from the group consisting of Cry endotoxins set forth in Table 2. 
     47. The nucleic acid molecule of embodiment 38, wherein the second amino acid sequence comprises an amino acid sequence selected from the group consisting of SEQ ID NOS: 8, 10, 12 and 14. 
     48. The nucleic acid molecule of any one of embodiments 38-47, wherein the chimeric pesticidal polypeptide further comprises a linker amino sequence operably linked between the first amino acid sequence and the second amino acid sequence. 
     49. The nucleic acid molecule of embodiment 48, wherein the linker amino acid sequence is selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 28, 29, 30, and 31. 
     50. The nucleic acid molecule of any one of embodiments 38-49, wherein the chimeric pesticidal polypeptide comprises increased pesticidal activity against at least one pest, when compared to the pesticidal activity of the Cry endotoxin against the at least one pest. 
     51. The nucleic acid molecule of any one of embodiments 38-50, wherein the at least one pest is an insect pest. 
     52. The nucleic acid molecule of embodiment 51, wherein the insect pest is an insect pest from the order Coleoptera or Lepidoptera. 
     53. The nucleic acid molecule of embodiment 51, wherein the insect pest is from the genus  Diabrotica.    
     54. The nucleic acid molecule of embodiment 51, wherein the insect pest is western corn rootworm. 
     55. The nucleic acid molecule of embodiment 51, wherein the insect pest is black cutworm. 
     56. An expression cassette comprising a promoter that drives expression in a host cell operably linked to a nucleic acid molecule of any one of embodiments 38-55. 
     57. The expression cassette of embodiment 56, wherein the host cell is a plant cell. 
     58. The expression cassette of embodiment 57, wherein the promoter is selected from the group consisting of a chemical-inducible promoter, constitutive promoter, pest-inducible promoter, tissue-specific promoter and wound-inducible promoter. 
     59. The expression cassette of embodiment 58, wherein the tissue-specific promoter is selected from the group consisting of a leaf-preferred promoter, root-preferred promoter, seed-preferred promoter, stalk-preferred promoter and vascular tissue-preferred promoter. 
     60. A vector comprising the expression cassette of any one of embodiments 56-59. 
     61. A transformed plant, plant part, plant cell or seed comprising in its genome the expression cassette of any one of embodiments 56-60. 
     62. The transformed plant, plant part or plant host cell of embodiment 61, wherein the nucleotide sequence is stably incorporated into the genome of the transformed plant, plant part, plant cell or seed. 
     63. A pesticidal composition comprising an effective amount of a chimeric pesticidal polypeptide of any one of embodiments 21-37 or an active variant or fragment thereof having pesticidal activity. 
     64. The pesticidal composition of embodiment 63, further comprising bacteria expressing a nucleotide sequence encoding the chimeric pesticidal polypeptide or a biologically active fragment or variant thereof. 
     65. The pesticidal composition of embodiment 63, further comprising at least one agricultural protectant selected from the group consisting of an acaricide, bactericide, fertilizer or micronutrient donor, fungicide, insecticide, nematocide and semiochemical. 
     66. The pesticidal composition of embodiment 65, wherein the semiochemical is selected from the group consisting of an allomone, attractant, feeding pheromone, kairomone, repellent and stimulant. 
     67. A method of protecting a plant from an insect pest, the method comprising providing an effective amount of a pesticidal composition of any one of embodiments 63-66 to reduce insect pest-related damage to the plant. 
     68. The method of embodiment 67, wherein the pesticidal composition is applied by a procedure selected from the group consisting of spraying, dusting, broadcasting and seed coating. 
     69. The method of embodiment 67 or 68, wherein the chimeric pesticidal polypeptide has pesticidal activity against an insect pest in the order Coleoptera, an insect pest in the order Lepiedoptera or both. 
     70. The method of any one of embodiments 67-69, wherein the insect pest is selected from the group consisting of species in the genus  Diabrotica.    
     71. A plant comprising a polynucleotide construct stably incorporated in its genome, the polynucleotide construct comprising a nucleotide sequence operably linked to a promoter that drives expression in the plant, wherein the nucleotide sequence encodes a chimeric pesticidal polypeptide comprising a first amino acid sequence of a solubility-enhancing polypeptide operably linked to a second amino acid sequence of a Cry endotoxin. 
     72. The plant of embodiment 71, wherein the nucleotide sequence is selected from the group consisting of:
         (a) the nucleotide sequence set forth in SEQ ID NO: 1, 15, 17, 20, 22, 24, 26 or 32;   (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 2, 16, 18, 21, 23, 25, 27 or 33;   (c) a nucleotide sequence comprising at least 80% nucleotide sequence identity to SEQ ID NO: 1, 15, 17, 20, 22, 24, 26 or 32, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; and   (e) a nucleotide sequence encoding an amino acid sequence comprising at least 80% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 2, 16, 18, 21, 23, 25, 27 or 33, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.       

     73. The plant of embodiment 71 or 72, wherein the solubility-enhancing polypeptide is MBP. 
     74. The plant of embodiment 73, wherein the MBP is selected from the group consisting of MBPs having an accession number set forth in Table 1. 
     75. The plant of embodiment 71 or 72, wherein the solubility-enhancing protein is NusA. 
     76. The plant of embodiment 71 or 72, wherein the solubility-enhancing protein is thioredoxin. 
     77. The plant of embodiment 71, wherein the first amino acid sequence comprises an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 4, 6, 34, 35 and 36. 
     78. The plant of any one of embodiments 71-77, wherein the Cry endotoxin is selected from the group consisting of Cry endotoxins set forth in Table 2. 
     79. The plant of any one of embodiments 71-78, wherein the second amino acid sequence encodes a Cry endotoxin selected from the group consisting of Cry endotoxins set forth in Table 2. 
     80. The plant of embodiment 71 or 72, wherein the second amino acid sequence comprises an amino acid sequence selected from the group consisting of SEQ ID NOS: 8, 10, 12 and 14. 
     81. The plant of any one of embodiments 71-80, wherein the chimeric pesticidal polypeptide further comprises a linker amino sequence operably linked between the first amino acid sequence and the second amino acid sequence. 
     82. The nucleic acid molecule of embodiment 81, wherein the linker amino acid sequence is selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 28, 29, 30 and 31. 
     83. The plant of any one of embodiments 71-82, wherein the chimeric pesticidal polypeptide comprises increased pesticidal activity against at least one pest, when compared to the pesticidal activity of the Cry endotoxin against the at least one pest. 
     84. The plant of embodiment 83, wherein the at least one pest is an insect pest. 
     85. The plant of embodiment 84, wherein the insect pest is an insect pest from the order Coleoptera or Lepidoptera. 
     86. The plant of embodiment 84, wherein the insect pest is from the genus  Diabrotica.    
     87. The plant of embodiment 84, wherein the insect pest is western corn rootworm. 
     88. The plant of embodiment 84, wherein the insect pest is black cutworm. 
     89. The plant of any one of embodiments 71-88, wherein the plant is a monocot. 
     90. The plant of embodiment 89, wherein the monocot is barley, maize, rice, rye, sorghum, sugarcane or wheat. 
     91. The plant of any one of embodiments 71-88, wherein the plant is a dicot. 
     92. The plant of embodiment 91, wherein the dicot is alfalfa,  Brassica,  cotton, soybean or sunflower. 
     93. The plant of any one of embodiment 71-92, wherein the plant is a seed. 
     94. A method of protecting a plant, plant part or plant host cell from an insect pest, the method comprising the steps of:
         (a) introducing into the plant, plant part or plant host cell an expression cassette of any one of embodiments 56-59; and   (b) regenerating the plant, plant part or plant host cell into a morphologically normal fertile plant, wherein the plant or part thereof comprises a chimeric pesticidal polypeptide.       

     95. The method of embodiment 94, wherein the chimeric pesticidal polypeptide has pesticidal activity against an insect pest in the order Coleoptera, an insect pest in the order Lepidoptera or both. 
     96. The method of embodiment 94, wherein the insect pest is selected from the group consisting of species in the genus  Diabrotica.    
     97. A method of enhancing the resistance of a plant to at least one pest, the method comprising introducing into a plant or at least one plant cell a polynucleotide construct comprising a nucleotide sequence operably linked to a promoter that drives expression in the plant, wherein the nucleotide sequence encodes a chimeric pesticidal polypeptide comprising a first amino acid sequence of a solubility-enhancing polypeptide operably linked to a second amino acid sequence of a Cry endotoxin. 
     98. The method of embodiment 97, wherein the nucleotide sequence comprises a nucleotide sequence selected from the group consisting of:
         (a) the nucleotide sequence set forth in SEQ ID NO: 1, 15, 17, 20, 22, 24, 26 or 32;   (b) a nucleotide sequence encoding an amino acid sequence comprising the amino acid sequence set forth in SEQ ID NO: 2, 16, 18, 21, 23, 25, 27 or 33;   (c) a nucleotide sequence comprising at least 80% nucleotide sequence identity to SEQ ID NO: 1, 15, 17, 20, 22, 24, 26 or 32, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; and   (e) a nucleotide sequence encoding an amino acid sequence comprising at least 80% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 2, 16, 18, 21, 23, 25, 27 or 33, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.       

     99. The method of embodiment 97 or 98, wherein solubility-enhancing polypeptide is MBP. 
     100. The method of embodiment 99, wherein the MBP is selected from the group consisting of MBPs having an accession number set forth in Table 1. 
     101. The method of embodiment 97 or 98, wherein the solubility-enhancing protein is NusA. 
     102. The method of embodiment 97 or 98, wherein the solubility-enhancing protein is thioredoxin. 
     103. The method of embodiment 97, wherein the first amino acid sequence comprises an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 4, 6, 34, 35 and 36. 
     104. The method of any one of embodiments 97-103, wherein the Cry endotoxin is selected from the group consisting of Cry endotoxins set forth in Table 2. 
     105. The method of any one of embodiments 97-104, wherein the second amino acid sequence comprises an amino acid sequence selected from the group consisting of SEQ ID NOS: 8, 10, 12 and 14. 
     106. The method of any one of embodiments 97-105, wherein the chimeric pesticidal polypeptide further comprises a linker amino sequence operably linked between the first amino acid sequence and the second amino acid sequence. 
     107. The method of embodiment 106, wherein the linker amino acid sequence is selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 28, 29, 30 and 31. 
     108. The method of any one of embodiments 97-107, wherein the chimeric pesticidal polypeptide comprises increased pesticidal activity against at least one pest, when compared to the pesticidal activity of the Cry endotoxin against the at least one pest. 
     109. The method of any one of embodiments 97-108, further comprising regenerating a plant comprising the polynucleotide construct. 
     110. The method of any one of embodiments 97-109, wherein the plant has enhanced resistance to at least one pest, when compared to a control plant lacking the polynucleotide construct. 
     111. The method of embodiment 110, wherein the at least one pest is an insect pest. 
     112. The method of embodiment 111, wherein the insect pest is an insect pest from the order Coleoptera or Lepidoptera. 
     113. The method of embodiment 111, wherein the insect pest is from the genus  Diabrotica.    
     114. The method of embodiment 111, wherein the insect pest is western corn rootworm. 
     115. The method of embodiment 111, wherein the insect pest is black cutworm. 
     116. The method of any one of embodiments 97-115, wherein the plant is a monocot. 
     117. The method of embodiment 116, wherein the monocot is barley, maize, rice, rye, sorghum, sugarcane or wheat. 
     118. The method of any one of embodiments 97-115, wherein the plant is a dicot. 
     119. The method of embodiment 118, wherein the dicot is alfalfa, Brassica, cotton, soybean or sunflower. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       The present invention will be better understood and features, aspects and advantages other than those set forth above will become apparent when consideration is given to the following detailed description thereof. Such detailed description makes reference to the following drawings, wherein: 
         FIG. 1  depicts the structure of  E. coli  maltose-binding protein (MBP)-Bt Cry protein fusion of the present invention.  =maltose binding protein  =Bt Cry protein. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The present inventions now will be described more fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the inventions are shown. Indeed, these inventions may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will satisfy applicable legal requirements. Like numbers refer to like elements throughout. 
     Many modifications and other embodiments of the inventions set forth herein will come to mind to one skilled in the art to which these inventions pertain having the benefit of the teachings presented in the foregoing descriptions and the associated drawings. Therefore, it is to be understood that the inventions are not to be limited to the specific embodiments disclosed and that modifications and other embodiments are intended to be included within the scope of the appended claims. Although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation. 
     The present invention is based on the discovery that pesticidal activity of Cry endotoxins can be enhanced by operably linking a solubility-enhancing polypeptide, such as, for example, a maltose-binding protein (MBP), to a Cry endotoxin or insecticidally active fragment thereof. Moreover, insect pest resistance can be overcome by operably linking an MBP to a Cry endotoxin by the methods disclosed herein. The present invention therefore provides compositions and methods for enhancing Cry endotoxin activity, for overcoming plant pest resistance, and for protecting plants from pests, especially insect pests. 
     While the invention does not depend on a particular biological mechanism, the fusion of a solubility-enhancing polypeptide to a Cry endotoxin or insecticidally active fragment thereof may act to raise the solubility of the Cry protein, particularly in the insect gut environment, more particularly in the gut environment of a Coleopteran insect. That is the solubility of the fusion protein has a higher solubility at a slightly acidic pH than the Cry protein has at the same pH. In a preferred embodiment of the invention, with activated Cyr toxin that is used in making the fusion protein of the invention has an isoelectric point (pI) from about pH 7 to about pH 8. It is recognized that in the digestive system in Lepidopteran insects, pH is relatively high. In such alkaline conditions, an activated Bt Cry toxin typically is soluble but in the slightly acidic pH of the Coleopteran gut environment in which pH is slightly acidic, solubility of the activated Bt Cry toxin can be much lower. 
     Compositions and methods are provided for enhancing the pesticidal activity of Cry endotoxins by operably linking a solubility-enhancing protein to a Cry endotoxin and for protecting plants from insect pests. The compositions and methods disclosed herein include recombinant nucleic acid molecules that encode chimeric pesticidal polypeptides, expression cassettes and other nucleotide constructs including the nucleic acid molecules described herein organisms transformed with the nucleic acid molecules described herein, isolated chimeric pesticidal polypeptides, and pesticidal compositions having the chimeric pesticidal polypeptides, as well as methods of using the same. The compositions and methods therefore find use in enhancing pesticidal activity of pesticidal proteins and in protecting plants from insect pests. 
     As used herein, a “solubility-enhancing polypeptide” is any polypeptide that when operably linked to a Cry endotoxin or insecticidally active fragment thereof enhances the pesticidal activity of the Cry endotoxin or insecticidally active fragment against at least one insect pest, preferably a Coleopteran insect pest, when compared to the pesticidal activity of the same Cry endotoxin or insecticidally active fragment thereof in the absence of an operably linked solubility-enhancing polypeptide. In certain embodiments, the solubility enhancing polypeptides of the present invention are capable of increasing the solubility of an operable linked Cry endotoxin or insecticidally active fragment thereof at a slightly acidic pH that is found, for example, in the gut environment of a Coleopteran insect. Preferably, a chimeric pesticidal polypeptide of the present invention, which comprises a solubility-enhancing polypeptide of the present invention operably linked to a Cry endotoxin or insecticidally active fragment thereof, comprises an increased solubility in an environment that has a slightly acidic pH such as, for example, the gut environment of a Coleopteran insect, than the same Cry endotoxin or insecticidally active fragment thereof lacking the operably linked solubility-enhancing polypeptide. 
     As used herein, “pest” or “plant pest” means an organism that interferes with or is harmful to plant development and/or growth. Such plant pests include, but are not limited to, nematodes, insect, viruses, viroids, mites, fungal pathogens, bacteria and any other plant pests disclosed herein. Accordingly, the polynucleotides and polypeptides of the invention can be used to enhance resistance of plants to plant pests. One of skill in the art, however, understands that not all polypeptides are equally effective against all plant pests. The chimeric pesticidal polypeptides described herein display activity against plant pests such as insect pests, which may include economically important agronomic, forest, greenhouse, nursery ornamentals, food and fiber, public and animal health, domestic and commercial structure, household and stored product pests. Therefore, of interest herein are chimeric pesticidal polypeptides for use in protecting plants from insect pests. 
     As used herein, “pesticidal polypeptide” means a peptide, polypeptide or protein that has biological activity against insect pests (i.e., is pesticidal). 
     As used herein, “pesticidal” or “pesticidal activity” means capable of killing insect pests. Likewise, “pesticidal” or “pesticidal activity” means capable of inhibiting insect growth. As such, the chimeric pesticidal polypeptides described herein are capable of inhibiting growth or reproduction of or of killing, at least one plant pest, particularly at least one insect pest. 
     By “insecticidal activity” is intended the ability of a polypeptide of the invention or a composition comprising the polypeptide (or other agent, chemical or composition) to inhibit the growth of or damage to a plant caused by, at least one insect pest. 
     As used herein, “enhance” and the like means increasing an activity/effectiveness of a pesticidal polypeptide such as a Cry endotoxin against a particular pest by operably linking the Cry endotoxin to a solubility-enhancing polypeptide, where the activity/effectiveness of the resulting chimeric pesticidal polypeptide is increased by about 1% to about 5%, about 5% to about 10%, about 10% to about 20%, about 20% to about 30%, about 30% to about 40%, about 40% to about 50%, about 50% to about 60%, about 60% to about 70%, about 70% to about 80%, about 80% to about 90% or about 90% to about 100% when compared to a wild-type Cry endotoxin. Alternatively, the activity/effectiveness of the chimeric pesticidal polypeptide is increased by about 1%, about 2%, about 3%, about 4%, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or more when compared to the wild-type Cry endotoxin. In addition, “enhance” can mean that the host pest range of the pesticidal polypeptide is expanded to include additional pests. 
     As used herein, “about” means within a statistically meaningful range of a value such as a stated concentration, length, molecular weight, percentage, pH, time frame, temperature or volume. Such a value or range can be within an order of magnitude, typically within 20%, more typically within 10%, and even more typically within 5% of a given value or range. The allowable variation encompassed by “about” will depend upon the particular system under study, and can be readily appreciated by one of skill in the art. 
     As used herein, “chimeric polypeptide” or “chimeric pesticidal polypeptide” means a polypeptide having a first amino acid sequence derived from a first source operably linked, covalently or non-covalently, to a second amino acid sequence derived from a second source, where the first and second source are not the same. A first source and a second source that are not the same can include two different organisms or two different proteins from the same organism or a biological source and a synthetic source or even two different synthetic sources. A biological source can include any non-synthetically produced nucleotide or amino acid sequence (e.g., a genomic or cDNA sequence, a plasmid or viral vector, a native virion or a mutant or analog). A synthetic source can include a nucleotide or amino acid sequence produced chemically and not by a biological source (e.g., solid phase synthesis of amino acid sequences). The chimeric pesticidal polypeptide can be produced by expressing a recombinant nucleic add molecule encoding a polypeptide having at least two parts or can be produced synthetically. 
     A chimeric pesticidal polypeptide of the present can further comprise, for example, a linker molecule (also referred to here as a “linker) between the first and second amino acid sequences. Examples of linkers that can be used in the methods of the present invention are the NEB pMAL, SA, NusA, and TrxA linkers having the amino acid sequences set forth in SEQ ID NOS: 28, 29, 30, and 31, respectively. 
     Solubility-enhancing polypeptides of the present include, but are not limited to, maltose-binding protein (MBP), thioredoxin (e.g., TrxA), transcription elongation factor NusA, glutathione-S-transferase (GST), mistic, small ubiquitin-related modifier (SUMO), protein disulfide isomerase DsbC, and thiol:disulfide interchange protein DsbD, and variants and fragments thereof that when operably linked to a Cry endotoxin or insecticidally active fragment thereof enhance the pesticidal activity of an operably linked Cry endotoxin or insecticidally active fragment against at least one insect pest, preferably a Coleopteran insect pest, when compared to the pesticidal activity of the same Cry endotoxin or insecticidally active fragment thereof in the absence of an operably linked solubility-enhancing polypeptide. 
     As used herein, “maltose-binding protein” or “MBP” means a polypeptide that is a member of the maltodextrin transport system that binds maltodextrins (e.g., maltose, maltotriose and trehalose) with micromolar affinity and that is essential for an energy-dependent translocation of maltodextrins through a cytoplasmic membrane of some prokaryotes. See, Boos &amp; Shuman (1998)  Microbiol. Mol. Biol. Rev.  62:204-229. For example, in  Escherichia coli,  the maIE gene encodes a 396 amino acid residue pre-MBP, which subsequently is processed into MBP upon cleavage of a 26 amino acid N-terminal signal peptide. See, e.g., Duplay et al. (1984)  J. Biol. Chem.  259:10606-10613. 
     In one embodiment of the invention, the chimeric pesticidal polypeptide comprises an amino acid sequence of an MBP operably linked to an amino acid sequence of a Cry endotoxin including, for example, the chimeric pesticidal polypeptides comprising the amino acid sequences set forth in SEQ ID NOS: 2, 21, 23, 25, 27, and 33 and encoded by the nucleotide sequences set forth in SEQ ID NOS: 1, 20, 22, 24, 26, and 32, respectively. 
     In another embodiment, the chimeric pesticidal polypeptide comprises an amino acid sequence of NusA operably linked to an amino acid sequence of a Cry endotoxin including, for example, the chimeric pesticidal polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 16 and encoded by the nucleotide sequence set forth in SEQ ID NO: 15. 
     In yet another embodiment, the chimeric pesticidal polypeptide comprises an amino acid sequence of thioredoxin operably linked to an amino acid sequence of a Cry endotoxin including, for example, the chimeric pesticidal polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 18 and encoded by the nucleotide sequence set forth in SEQ ID NO: 17. 
     The present invention comprises the use of an solubility-enhancing polypeptide that when fused to a Cry endotoxin polypeptide increases the pesticidial activity of against at least one pest, preferably an insect pest, more preferably an insect pest from the order Coleoptera or Lepidoptera, even more preferably an insect pest from the genus  Diabrotica,  most preferably the western corn rootworm ( Diabrotica virgifera virgifera ). In certain embodiments, the solubility-enhancing polypeptide selected from the group consisting of MBP, NusA, and TrxA. 
     The present invention does not depend on the use of a particular solubility-enhancing polypeptide. Any solubility-enhancing polypeptide that, when fused to a Cry endotoxin polypeptide, is capable increasing the pesticidial activity of the Cry endotoxin polypeptide against at least one pest can be used in the methods and compositions disclosed herein. Such solubility enhancing polypeptides include, for example, pre-proteins and mature proteins, and variants and fragments thereof. In certain embodiments, the solubility-enhancing polypeptide is MBP. MBPs of the present invention include, but are not limited, full-length MBP (also referred to as “pre-MBP”, a mature form of an MBP, a fragment of a full-length or mature MBP or a variant. Unless expressed stated herein or otherwise apparent form the context, the terms “maltose-binding protein” and “MBP” encompass such full-length and mature forms of MBPs as well as fragments and variants thereof. 
     The amino acid and/or nucleotide sequences for a number of MBPs have been disclosed and can be used in the compositions and methods of the present invention including, but not limited to, the following those MBPs having the following accession numbers in Table 1. 
     
       
         
           
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 GenBank Accession Number* 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 NP_290668 
                 ZP_03064501 
                 CBG37227 
                 NP_709885 
                 ACI74011 
               
               
                 YP_002415175 
                 YP_001726921 
                 EFK20320 
                 ZP_02999303 
                 YP_312946 
               
               
                 ZP_06651607 
                 ACC91724 
                 NP_756856 
                 ACF57854 
                 ACF57853 
               
               
                 ABO28850 
                 AAB86559 
                 AAC83813 
                 BAI57431 
                 AAQ93661 
               
               
                 ACI46135 
                 ACI46133 
                 AAK55118 
                 AAB87675 
                 ZP_02904048 
               
               
                 YP_002385139 
                 ZP_06356417 
                 YP_003367151 
                 YP_002639794 
                 NP_458527 
               
               
                 YP_001338045 
                 ACY91424 
                 ZP_03381315 
                 YP_219095 
                 AAX68014 
               
               
                 AAL23053 
                 NP_463094 
                 ZP_04558600 
                 YP_002228802 
                 ZP_02700756 
               
               
                 YP_002149143 
                 CAA38189 
                 CBK84491 
                 ZP_06390929 
                 YP_001455393 
               
               
                 YP_003610806 
                 YP_001174979 
                 YP_001572421 
                 YP_002241024 
                 P18815 
               
               
                 YP_003212142 
                 YP_001436222 
                 YP_001480694 
                 ZP_06712852 
                 ZP_04616043 
               
               
                 ZP_04638283 
                 ZP_06191619 
                 ZP_04626936 
                 ZP_04633703 
                 ZP_04613648 
               
               
                 NP_667372 
                 ZP_04641958 
                 YP_001161578 
                 YP_001008013 
                 ZP_04625082 
               
               
                 YP_003294268 
                 YP_002931694 
                 ZP_06638363 
                 BAE44434 
                 YP_003743846 
               
               
                 YP_003039205 
                 NP_927811 
                 ZP_05729913 
                 YP_003714385 
                 YP_003469920 
               
               
                 YP_003729949 
                 YP_003520206 
                 YP_002891253 
                 ZP_03366279 
                 ZP_05920010 
               
               
                 YP_003007381 
                 ZP_06636620 
                 YP_001343631 
                 YP_003255618 
                 ZP_04976824 
               
               
                 YP_089260 
                 ZP_06157313 
                 YP_002474876 
                 YP_001447347 
                 ZP_01984309 
               
               
                 ZP_04754507 
                 ZP_06177188 
                 NP_800911 
                 ZP_06180148 
                 ZP_02477508 
               
               
                 ZP_01258612 
                 ZP_05878943 
                 ZP_05629984 
                 YP_001053928 
                 YP_132094 
               
               
                 ZP_05882865 
                 ZP_02194153 
                 ZP_01221443 
                 ZP_05927269 
                 NP_763457 
               
               
                 ZP_05943387 
                 ZP_01865449 
                 ZP_05717553 
                 ZP_06032225 
                 ZP_06079586 
               
               
                 ZP_05239461 
                 YP_002812521 
                 NP_233329 
                 ZP_01970977 
                 ZP_04918366 
               
               
                 ZP_05719582 
                 ZP_05886194 
                 ZP_05117778 
                 ZP_06941932 
                 ZP_04415908 
               
               
                 ZP_01950896 
                 ZP_01676462 
                 ZP_01897017 
                 YP_001343632 
                 YP_132089 
               
               
                 ZP_06938154 
                 YP_003007380 
                 ZP_03352864 
                 ZP_01221449 
                 ZP_00988394 
               
               
                 ZP_01811558 
                 YP_856203 
                 YP_001142458 
                 NP_936112 
                 ZP_01062901 
               
               
                 NP_763137 
                 YP_206757 
                 ZP_00988462 
                 ZP_04403894 
                 ZP_05120494 
               
               
                 ZP_01947956 
                 ZP_01062959 
                 ZP_01865441 
                 YP_431775 
                 ZP_01895948 
               
               
                 YP_001173956 
                 ZP_03375554 
                 YP_001188617 
                 ZP_00135549 
                 ZP_03699982 
               
               
                 YP_003269532 
                 YP_003548264 
                 ZP_01893753 
                 ZP_01112736 
                 YP_431868 
               
               
                 ZP_01157584 
                 ZP_01115171 
                 ZP_03369842 
                 ZP_01955972 
                 ZP_01955970 
               
               
                 ZP_06938864 
                 YP_002352611 
                 YP_002250432 
                 YP_002534909 
                 NP_229009 
               
               
                 YP_001245135 
                 YP_002534307 
                 YP_001739017 
                 YP_001244560 
                 YP_003346096 
               
               
                 YP_002250571 
                 CAA72193 
                 NP_229635 
                 YP_002352749 
                 YP_001470540 
               
               
                 NP_623418 
                 ZP_05493657 
                 YP_001662811 
                 YP_002941091 
                 YP_003672376 
               
               
                 YP_146557 
                 ZP_04392773 
                 YP_003677351 
                 ZP_05336993 
                 YP_003477456 
               
               
                 AAY89718 
                 YP_001568299 
                 YP_001410291 
                 ZP_03385039 
                 YP_001568464 
               
               
                 YP_001124738 
                 YP_604964 
                 YP_003514612 
                 ZP_03146818 
                 ZP_04152680 
               
               
                 ZP_04158384 
                 YP_001305848 
                 YP_001142178 
                 ZP_04307633 
                 YP_002509363 
               
               
                 ZP_06220504 
                 ZP_04121933 
                 ZP_04204735 
                 ZP_03232052 
                 YP_003148220 
               
               
                 ZP_04086064 
                 ZP_04258265 
                 ZP_04296475 
                 ZP_02397670 
                 ZP_00236932 
               
               
                 ZP_04187645 
                 ZP_03105903 
                 ZP_03238270 
                 YP_896372 
                 YP_038073 
               
               
                 YP_085351 
                 NP_846464 
                 ZP_04128078 
                 ZP_04285672 
                 YP_003186266 
               
               
                 ZP_02948002 
                 YP_002447583 
                 CAB65651 
                 NP_980358 
                 ZP_04302212 
               
               
                 ZP_03493519 
                 ZP_02878398 
                 ZP_04176050 
                 ZP_05183404 
                 ZP_04290906 
               
               
                 ZP_04199019 
                 ZP_04263630 
                 YP_001646638 
                 ZP_04229423 
                 ZP_04170363 
               
               
                 YP_002334500 
                 BAB40635 
                 ZP_04218748 
                 ZP_02866107 
                 YP_697032 
               
               
                 YP_003258733 
                 ZP_02953587 
                 YP_177014 
                 YP_051264 
                 ZP_02638879 
               
               
                 YP_003018517 
                 ZP_04607307 
                 ZP_03828721 
                 YP_003116555 
                 ZP_03832162 
               
               
                 YP_002316535 
                 NP_294284 
                 NP_563259 
                 YP_002785582 
                 YP_002886868 
               
               
                 ZP_06681977 
                 ZP_05667047 
                 ZP_05664221 
                 ZP_03981553 
                 ZP_06699376 
               
               
                 YP_001488817 
                 YP_001375943 
                 ZP_00603967 
                 ZP_05132228 
                 YP_699603 
               
               
                 YP_002574412 
                 YP_001814130 
                 YP_003445304 
                 ZP_04878976 
                 ZP_03055079 
               
               
                 ZP_01861955 
                 YP_002918149 
                 YP_002240053 
                 YP_001334109 
                 YP_001813248 
               
               
                 ZP_06549508 
                 ZP_06329195 
                 ZP_04864724 
                 YP_001179261 
                 YP_144918 
               
               
                 YP_039671 
                 NP_645005 
                 NP_370738 
                 YP_003465347 
                 ZP_05877552 
               
               
                 ZP_04016117 
                 YP_001439475 
                 BAI87082 
                 ADI96692 
                 CBI48099 
               
               
                 YP_850342 
                 YP_005257 
                 ZP_00233442 
                 YP_003613139 
                 ZP_06315382 
               
               
                 YP_946709 
                 NP_391341 
                 AAD42742 
                 EFK15520 
                 ZP_05300115 
               
               
                 ZP_05298949 
                 YP_001695468 
                 ZP_02185856 
                 YP_830242 
                 YP_288893 
               
               
                 NP_471563 
                 YP_003208908 
                 YP_002958966 
                 YP_001836789 
                 ZP_01827852 
               
               
                 YP_415662 
                 NP_359509 
                 BAB18102 
                 YP_002739191 
                 YP_003154662 
               
               
                 ZP_03497002 
                 YP_003696753 
                 YP_003684577 
                 ZP_07053052 
                 ZP_05231671 
               
               
                 ZP_02711433 
                 ZP_01821820 
                 ZP_01821214 
                 NP_346527 
                 ZP_01408156 
               
               
                 ZP_02186034 
                 BAC10980 
                 YP_003336687 
                 NP_670540 
                 YP_071605 
               
               
                 ZP_06640983 
                 YP_003723548 
                 ZP_04853514 
                 YP_002743432 
                 YP_001399898 
               
               
                 YP_077886 
                 YP_002038696 
                 NP_391296 
                 ZP_06611384 
                 ZP_06198009 
               
               
                 YP_003339007 
                 YP_003729851 
                 ZP_03958786 
                 ZP_01170933 
                 YP_081353 
               
               
                 YP_001719722 
                 ZP_06059512 
                 YP_001449426 
                 ZP_06872846 
                 ZP_04851795 
               
               
                 YP_003241054 
                 YP_002473273 
                 YP_001396549 
                 YP_920853 
                 ZP_06190079 
               
               
                 BAI87023 
                 YP_001664668 
                 YP_003704868 
                 ZP_06899649 
                 NP_693481 
               
               
                 YP_003314160 
                 YP_003334475 
                 ZP_05647136 
                 YP_003203952 
                 YP_056254 
               
               
                 ZP_06014674 
                 ZP_05687198 
                 YP_003506902 
                 ZP_04449975 
                 YP_002958266 
               
               
                 YP_002948859 
                 YP_003677653 
                 ZP_06428097 
                 YP_003781558 
                 ZP_03925962 
               
               
                 YP_001199499 
                 NP_579667 
                 YP_003003618 
                 YP_003678645 
                 ZP_03225712 
               
               
                 ZP_01460386 
                 YP_003326738 
                 YP_002744243 
                 YP_002746719 
                 YP_002123617 
               
               
                 AAA26922 
                 YP_003477807 
                 ZP_02329884 
                 ZP_04431587 
                 ZP_01461944 
               
               
                 NP_242885 
                 YP_003161478 
                 ZP_06533915 
                 YP_001137791 
                 YP_003009537 
               
               
                 NP_125870 
                 YP_002308182 
                 ZP_06885100 
                 ZP_04876799 
                 YP_001559408 
               
               
                 YP_003699585 
                 ZP_01130620 
                 YP_184184 
                 ZP_05621839 
                 NP_243792 
               
               
                 YP_002883083 
                 YP_003637265 
                 YP_003425427 
                 YP_003061750 
                 YP_002997014 
               
               
                 ZP_07076764 
                 ZP_05749331 
                 YP_602654 
                 NP_269430 
                 NP_737480 
               
               
                 ZP_06808450 
                 YP_003009727 
                 YP_002565304 
                 YP_598732 
                 YP_280516 
               
               
                 YP_002881503 
                 NP_784007 
                 YP_002246441 
                 ZP_02045060 
                 YP_002509787 
               
               
                 ZP_06608272 
                 YP_002136599 
                 ZP_06363693 
                 EFK02214 
                 ZP_05431814 
               
               
                 YP_136875 
                 ZP_06662656 
                 YP_001880661 
                 YP_177522 
                 ZP_06048003 
               
               
                 YP_002387348 
                 ZP_01983428 
                 YP_003229678 
                 ZP_04402673 
                 YP_467337 
               
               
                 ZP_06365288 
                 CAL69747 
                 YP_002881799 
                 ZP_04412511 
                 ZP_01951248 
               
               
                   
               
               
                 *The amino acid and corresponding nucleotide sequences of the accession numbers in Table 1 are herein incorporated by reference. 
               
            
           
         
       
     
     In certain embodiments of the invention, the MBP comprises the amino acid sequence set forth in SEQ ID NO: 4 or 6 or fragment or variant thereof. Any nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 4 or 6 or fragment or variant thereof, can be used in the methods and compositions of the present invention. In some embodiments of the invention, the nucleotide sequences of the invention will be optimized for expression in a host organism or cell of interest, particularly a plant, more particularly a crop plant, most particularly a maize plant. 
     As used herein, “Cry endotoxin” means a δ-endotoxin encoded by cry (crystal protein) genes that are located mainly on large plasmids in members of the genus  Bacillus,  although chromosomally encoded Cry endotoxins have been reported. See, Ben-Dov et al. (1996)  Appl. Environ. Microbiol.  62:3140-3145; Berry et al. (2002)  Appl. Environ. Microbiol.  68:5082-5095; Gonzáles et al. (1981)  Plasmid  5:351-365; Lereclus et al. (1982)  Mol. Gen. Genet.  186:391-398; and Trisrisook et al. (1990)  Appl. Environ. Microbiol.  56:1710-1716. Cry endotoxins do not have a broad spectrum of activity, so they typically do not kill beneficial insects. Furthermore, Cry endotoxins are non-toxic to mammals, including humans, domesticated animals and wildlife. 
     Cry endotoxins generally have five conserved sequence domains, and three conserved structural domains. See, e.g., de Maagd et al. (2001)  Trends Genetics  17:193-199. The first conserved structural domain (Domain I) consists of seven alpha helices and is involved in membrane insertion and pore formation. The second conserved structural domain (Domain II) consists of three beta-sheets arranged in a Greek key configuration, and the third conserved structural domain (Domain III) consists of two antiparallel beta-sheets in “jelly-roll” formation. Domains II and III are involved in receptor recognition and binding, and are therefore considered determinants of toxin specificity. 
     As used herein, “insect pest” means an organism in the phylum Arthropoda that interferes with or is harmful to plant development and/or growth, and more specifically means an organism in the class Insecta. The class Insecta can be divided into two groups historically treated as subclasses: (1) wingless insects, known as Apterygota; and (2) winged insects, known as Pterygota. The insect pests can be adults, larvae or even ova. A preferred developmental stage for testing for pesticidal activity is larvae or other immature form of the insect pest. Methods of rearing insect larvae and performing bioassays are well known in the art. See, e.g., Czapla &amp; Lang (1990)  J. Econ. Entomol.  83:2480-2485; Griffith &amp; Smith (1977)  J. Aust. Ent. Soc.  16:366; and Keiper &amp; Foote (1996)  Hydrobiologia  339:137-139; as well as U.S. Pat. No. 5,351,643. For example, insect pests can be reared in total darkness at about 20° C. to about 30° C. and from about 30% to about 70% relative humidity. 
     Compositions comprising chimeric pesticidal polypeptide-encoding nucleic acid molecules and chimeric pesticidal polypeptides also are provided. The compositions include chimeric pesticidal polypeptides that comprise a solubility-enhancing polypeptide fused to a Cry endotoxin or biologically active fragment thereof. Nucleotide sequences that encode the chimeric pesticidal polypeptides can be derived from the chimeric pesticidal polypeptide sequence and produced using any method known in the art. The compositions also include variants and fragments of the chimeric pesticidal-encoding nucleic acid molecules and chimeric pesticidal polypeptides. The isolated, chimeric pesticidal-encoding nucleic acid molecules can be used to create transgenic organisms, such as plants, that are resistant to an insect pest susceptible to the pesticidal polypeptide. 
     The presently disclosed methods and compositions provides for chimeric pesticidal polypeptides with improved efficacy and nucleic acid molecules encoding the chimeric pesticidal polypeptides. The chimeric pesticidal polypeptides comprise solubility-enhancing polypeptide fused (i.e., operably linked) to a Cry endotoxin or biologically active fragment thereof and have enhanced pesticidal activity against at least one plant pest, when compared to the pesticidal activity of the Cry endotoxin or biologically active fragment thereof that has not been fused to a solubility-enhancing polypeptide. 
     As used herein, “pesticide,” “pesticidal polypeptide,” or “pesticidal protein” mean a polypeptide that is capable of killing the pest or inhibiting its growth, feeding or reproduction. One of skill in the art understands that not all substances or mixtures thereof are equally effective against all pests. “Chimeric pesticidal polypeptides” or “chimeric pesticidal proteins” are “pesticidal polypeptides” or “pesticidal proteins”, to which a solubility-enhancing polypeptide has been fused as disclosed herein. Of particular interest herein are pesticidal polypeptides and chimeric pesticidal polypeptides that act as insecticides and thus have biological activity against insect pests. 
     As used herein, “pest” means an organism that interferes with or is harmful to plant development and/or growth. Examples of pests include, but are not limited to, algae, arachnids (e.g., acarids including mites and ticks), bacteria (e.g., plant pathogens including  Xanthomonas  spp. and  Pseudomonas  spp.), crustaceans (e.g., pillbugs and sowbugs); fungi (e.g., members in the phylum Ascomycetes or Basidiomycetes, and fungal-like organisms including Oomycetes such as  Pythium  spp. and  Phytophthora  spp.), insects, mollusks (e.g., snails and slugs), nematodes (e.g., soil-transmitted nematodes including  Clonorchis  spp.,  Fasciola  spp.,  Heterodera  spp.,  Globodera  spp.,  Opisthorchis  spp. and  Paragonimus  spp.), protozoans (e.g.,  Phytomonas  spp.), viruses (e.g.,  Comovirus  spp.,  Cucumovirus  spp.,  Cytorhabdovirus  spp.,  Luteovirus  spp.,  Nepovirus  spp.,  Potyvirus  spp.,  Tobamovirus  spp.,  Tombusvirus  spp. and  Tospovirus  spp.), viroids, parasitic plants, and weeds. 
     Of particular interest herein are insect pests. As used herein, “insect pest” means an organism in the phylum Arthropoda that interferes with or is harmful to plant development and/or growth, and more specifically means an organism in the class Insecta. The class Insecta can be divided into two groups historically treated as subclasses: (1) wingless insects, known as Apterygota; and (2) winged insects, known as Pterygota. Examples of insect pests include, but are not limited to, insects in the orders Coleoptera, Diptera, Hemiptera, Homoptera, Hymenoptera, Isoptera, Lepidoptera, Mallophaga orthroptera, Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera and Thysanura, particularly Coleoptera and Lepidoptera. While technically not insects, arthropods such as arachnids, especially in the order Acari, are included in “insect pest.” Insect pests include economically important agronomic, forest, greenhouse, nursery ornamentals, food and fiber, public and animal health, domestic and commercial structure, household, and stored product pests. 
     Insects of the order Lepidoptera include, but are not limited to, armyworms, cutworms, loopers, and heliothines in the family Noctuidae  Agrotis ipsilon  Hufnagel (black cutworm);  A. orthogonia  Morrison (western cutworm);  A. segetum  Denis &amp; Schiffermüller (turnip moth);  A. subterranea  Fabricius (granulate cutworm);  Alabama argillacea  Hübner (cotton leaf worm);  Anticarsia gemmatalis  Hübner (velvetbean caterpillar);  Athetis mindara  Barnes and McDunnough (rough skinned cutworm);  Earias insulana  Boisduval (spiny bollworm);  E. vittella  Fabricius (spotted bollworm);  Egira  ( Xylomyges )  curialis  Grote (citrus cutworm);  Euxoa messoria  Harris (darksided cutworm);  Helicoverpa armigera  Hübner (American bollworm);  H. zea  Boddie (corn earworm or cotton bollworm);  Heliothis virescens  Fabricius (tobacco budworm);  Hypena scabra  Fabricius (green cloverworm);  Hyponeuma taltula  Schaus; ( Mamestra configurata  Walker (bertha armyworm);  M. brassicae  Linnaeus (cabbage moth);  Melanchra picta  Harris (zebra caterpillar);  Mocis latipes  Guenée (small mocis moth);  Pseudaletia unipuncta  Haworth (armyworm);  Pseudoplusia includens  Walker (soybean looper);  Richia albicosta  Smith (Western bean cutworm);  Spodoptera frugiperda  J E Smith (fall armyworm);  S. exigua  Hübner (beet armyworm);  S. litura  Fabricius (tobacco cutworm, cluster caterpillar);  Trichoplusia ni  Hübner (cabbage looper); borers, casebearers, webworms, coneworms, and skeletonizers from the families Pyralidae and Crambidae such as  Achroia grisella  Fabricius (lesser wax moth);  Amyelois transitella  Walker (naval orangeworm);  Anagasta kuehniella  Zeller (Mediterranean flour moth);  Cadra cautella  Walker (almond moth);  Chilo partellus  Swinhoe (spotted stalk borer);  C. suppressalis  Walker (striped stem/rice borer);  C. terrenellus  Pagenstecher (sugarcane stemp borer);  Corcyra cephalonica  Stainton (rice moth);  Crambus caliginosellus  Clemens (corn root webworm);  C. teterrellus  Zincken (bluegrass webworm);  Cnaphalocrocis medinalis  Guenée (rice leaf roller);  Desmia funeralis  Hübner (grape leaffolder);  Diaphania hyalinata  Linnaeus (melon worm);  D. nitidalis  Stoll (pickleworm);  Diatraea flavipennella  Box;  D. grandiosella  Dyar (southwestern corn borer),  D. saccharalis  Fabricius (sugarcane borer);  Elasmopalpus lignosellus  Zeller (lesser cornstalk borer);  Eoreuma loftini  Dyar (Mexican rice borer);  Ephestia elutella  Hübner (tobacco (cacao) moth);  Galleria mellonella  Linnaeus (greater wax moth);  Hedylepta accepta  Butler (sugarcane leafroller);  Herpetogramma licarsisalis  Walker (sod webworm);  Homoeosoma electellum  Hulst (sunflower moth);  Loxostege sticticalis  Linnaeus (beet webworm);  Maruca testulalis  Geyer (bean pod borer);  Orthaga thyrisalis  Walker (tea tree web moth);  Ostrinia nubilalis  Hübner (European corn borer);  Plodia interpunctella  Hübner (Indian meal moth);  Scirpophaga incertulas  Walker (yellow stem borer);  Udea rubigalis  Guenée (celery leaftier); and leafrollers, budworms, seed worms, and fruit worms in the family Tortricidae  Acleris gloverana  Walsingham (Western blackheaded budworm);  A. variana  Fernald (Eastern blackheaded budworm);  Adoxophyes orana  Fischer von Rösslerstamm (summer fruit tortrix moth);  Archips  spp. including  A. argyrospila  Walker (fruit tree leaf roller) and  A. rosana  Linnaeus (European leaf roller);  Argyrotaenia  spp.;  Bonagota salubricola  Meyrick (Brazilian apple leafroller);  Choristoneura  spp.;  Cochylis hospes  Walsingham (banded sunflower moth);  Cydia latiferreana  Walsingham (filbertworm);  C. pomonella  Linnaeus (codling moth);  Endopiza viteana  Clemens (grape berry moth);  Eupoecilia ambiguella  Hübner (vine moth);  Grapholita molesta  Busck (oriental fruit moth);  Lobesia botrana  Denis &amp; Schiffermüller (European grape vine moth);  Platynota flavedana  Clemens (variegated leafroller);  P. stultana  Walsingham (omnivorous leafroller);  Spilonota ocellana  Denis &amp; Schiffermüller (eyespotted bud moth); and  Suleima helianthana  Riley (sunflower bud moth). 
     Selected other agronomic pests in the order Lepidoptera include, but are not limited to,  Alsophila pometaria  Harris (fall cankerworm);  Anarsia lineatella  Zeller (peach twig borer);  Anisota senatoria  J. E. Smith (orange striped oakworm);  Antheraea pernyi  Guérin-Méneville (Chinese Oak Silkmoth);  Bombyx mori  Linnaeus (Silkworm);  Bucculatrix thurberiella  Busck (cotton leaf perforator);  Collas eurytheme  Boisduval (alfalfa caterpillar);  Datana integerrima  Grote &amp; Robinson (walnut caterpillar);  Dendrolimus sibiricus  Tschetwerikov (Siberian silk moth),  Ennomos subsignaria  Hübner (elm spanworm);  Erannis tiliaria  Harris (linden looper);  Erechthias flavistriata  Walsingham (sugarcane bud moth);  Euproctis chrysorrhoea  Linnaeus (browntail moth);  Harrisina americana  Guérin-Méneville (grapeleaf skeletonizer);  Heliothis subflexa  Guenée;  Hemileuca oliviae  Cockrell (range caterpillar);  Hyphantria cunea  Drury (fall webworm);  Keiferia lycopersicella  Walsingham (tomato pinworm);  Lambdina fiscellaria fiscellaria  Hulst (Eastern hemlock looper);  L. fiscellaria lugubrosa  Hulst (Western hemlock looper);  Leucoma salicis  Linnaeus (satin moth);  Lymantria dispar  Linnaeus (gypsy moth);  Malacosoma  spp.;  Manduca quinquemaculata  Haworth (five spotted hawk moth, tomato hornworm);  M. sexta  Haworth (tomato hornworm, tobacco hornworm);  Operophtera brumata  Linnaeus (winter moth);  Orgyia  spp.;  Paleacrita vernata  Peck (spring cankerworm);  Papilio cresphontes  Cramer (giant swallowtail orange dog);  Phryganidia californica  Packard (California oakworm);  Phyllocnistis citrella  Stainton (citrus leafminer);  Phyllonorycter blancardella  Fabricius (spotted tentiform leafminer);  Pieris brassicae  Linnaeus (large white butterfly);  P. rapae  Linnaeus (small white butterfly);  P. napi  Linnaeus (green veined white butterfly);  Platyptilia carduidactyla  Riley (artichoke plume moth);  Plutella xylostella  Linnaeus (diamondback moth);  Pectinophora gossypiella  Saunders (pink bollworm);  Pontia protodice  Boisduval &amp; Leconte (Southern cabbageworm);  Sabulodes aegrotata  Guenée (omnivorous looper);  Schizura concinna  J. E. Smith (red humped caterpillar);  Sitotroga cerealella  Olivier (Angoumois grain moth);  Telchin licus  Drury (giant sugarcane borer);  Thaumetopoea pityocampa  Schiffermüller (pine processionary caterpillar);  Tineola bisselliella  Hummel (webbing clothesmoth);  Tuta absoluta  Meyrick (tomato leafminer) and  Yponomeuta padella  Linnaeus (ermine moth). 
     Of interest are larvae and adults of the order Coleoptera including weevils from the families Anthribidae, Bruchidae, and Curculionidae including, but not limited to:  Anthonomus grandis  Boheman (boll weevil);  Cylindrocopturus adspersus  LeConte (sunflower stem weevil);  Diaprepes abbreviatus  Linnaeus (Diaprepes root weevil);  Hypera punctata  Fabricius (clover leaf weevil);  Lissorhoptrus oryzophilus  Kuschel (rice water weevil);  Metamasius hemipterus hemipterus  Linnaeus (West Indian cane weevil);  M. hemipterus sericeus  Olivier (silky cane weevil);  Sitophilus granarius  Linnaeus (granary weevil);  S. oryzae  Linnaeus (rice weevil);  Smicronyx fulvus  LeConte (red sunflower seed weevil);  S. sordidus  LeConte (gray sunflower seed weevil);  Sphenophorus maidis  Chittenden (maize billbug);  S. livis  Vaurie (sugarcane weevil);  Rhabdoscelus obscurus  Boisduval (New Guinea sugarcane weevil); flea beetles, cucumber beetles, rootworms, leaf beetles, potato beetles, and leafminers in the family Chrysomelidae including, but not limited to:  Chaetocnema ectypa  Horn (desert corn flea beetle);  C. pulicaria  Melsheimer (corn flea beetle);  Colaspis brunnea  Fabricius (grape colaspis);  Diabrotica barberi  Smith &amp; Lawrence (northern corn rootworm);  D. undecimpunctata howardi  Barber (southern corn rootworm);  D. virgifera virgifera  LeConte (western corn rootworm);  Leptinotarsa decemlineata  Say (Colorado potato beetle);  Oulema melanopus  Linnaeus (cereal leaf beetle);  Phyllotreta cruciferae  Goeze (corn flea beetle);  Zygogramma exclamationis  Fabricius (sunflower beetle); beetles from the family Coccinellidae including, but not limited to:  Epilachna varivestis  Mulsant (Mexican bean beetle); chafers and other beetles from the family Scarabaeidae including, but not limited to:  Antitrogus parvulus  Britton (Childers cane grub);  Cyclocephala borealis  Arrow (northern masked chafer, white grub);  C. immaculata  Olivier (southern masked chafer, white grub);  Dermolepida albohirtum  Waterhouse (Greyback cane beetle);  Euetheola humilis rugiceps  LeConte (sugarcane beetle);  Lepidiota frenchi  Blackburn (French&#39;s cane grub);  Tomarus gibbosus  De Geer (carrot beetle);  T. subtropicus  Blatchley (sugarcane grub);  Phyllophaga crinita  Burmeister (white grub);  P. latifrons  LeConte (June beetle);  Popillia japonica  Newman (Japanese beetle);  Rhizotrogus majalis  Razoumowsky (European chafer); carpet beetles from the family Dermestidae; wireworms from the family Elateridae,  Eleodes  spp.,  Melanotus  spp. including  M. communis  Gyllenhal (wireworm);  Conoderus  spp.;  Limonius  spp.;  Agriotes  spp.;  Ctenicera  spp.;  Aeolus  spp.; bark beetles from the family Scolytidae; beetles from the family Tenebrionidae; beetles from the family Cerambycidae such as, but not limited to,  Migdolus fryanus  Westwood (longhorn beetle); and beetles from the Buprestidae family including, but not limited to,  Aphanisticus cochinchinae seminulum  Obenberger (leaf-mining buprestid beetle). 
     Adults and immatures of the order Diptera are of interest, including leafminers  Agromyza parvicomis  Loew (corn blotch leafminer); midges including, but not limited to:  Contarinia sorghicola  Coquillett (sorghum midge);  Mayetiola destructor  Say (Hessian fly);  Neolasioptera murtfeldtiana  Felt, (sunflower seed midge);  Sitodiplosis mosellana  Géhin (wheat midge); fruit flies (Tephritidae),  Oscinella frit  Linnaeus (frit flies); maggots including, but not limited to:  Delia  spp. including  Delia platura  Meigen (seedcorn maggot);  D. coarctata  Fallen (wheat bulb fly);  Fannia canicularis  Linnaeus,  F. femoralis  Stein (lesser house flies);  Meromyza americana  Fitch (wheat stem maggot);  Musca domestica  Linnaeus (house flies);  Stomoxys calcitrans  Linnaeus (stable flies)); face flies, horn flies, blow flies,  Chrysomya  spp.;  Phormia  spp.; and other muscoid fly pests, horse flies  Tabanus  spp.; bot flies  Gastrophilus  spp.;  Oestrus  spp.; cattle grubs  Hypoderma  spp.; deer flies  Chrysops  spp.;  Melophagus ovinus  Linnaeus (keds); and other  Brachycera,  mosquitoes  Aedes  spp.;  Anopheles  spp.;  Culex  spp.; black flies  Prosimulium  spp.;  Simulium  spp.; biting midges, sand flies, sciarids, and other  Nematocera.    
     Included as insects of interest are those of the order Hemiptera such as, but not limited to, the following families: Adelgidae, Aleyrodidae, Aphididae, Asterolecaniidae, Cercopidae, Cicadellidae, Cicadidae, Cixiidae, Coccidae, Coreidae, Dactylopiidae, Delphacidae, Diaspididae, Eriococcidae, Flatidae, Fulgoridae, Issidae, Lygaeidae, Margarodidae, Membracidae, Miridae ortheziidae, Pentatomidae, Phoenicococcidae, Phylloxeridae, Pseudococcidae, Psyllidae, Pyrrhocoridae and Tingidae. 
     Agronomically important members from the order Hemiptera include, but are not limited to:  Acrosternum hilare  Say (green stink bug);  Acyrthisiphon pisum  Harris (pea aphid);  Adelges  spp. (adelgids);  Adelphocoris rapidus  Say (rapid plant bug);  Anasa tristis  De Geer (squash bug);  Aphis craccivora  Koch (cowpea aphid);  A. fabae  Scopoli (black bean aphid);  A. gossypii  Glover (cotton aphid, melon aphid);  A. maidiradicis  Forbes (corn root aphid);  A. pomi  De Geer (apple aphid);  A. spiraecola  Patch (spirea aphid);  Aulacaspis tegalensis  Zehntner (sugarcane scale);  Aulacorthum solani  Kaltenbach (foxglove aphid);  Bemisia tabaci  Gennadius (tobacco whitefly, sweetpotato whitefly);  B. argentifolii  Bellows &amp; Perring (silverleaf whitefly);  Blissus leucopterus leucopterus  Say (chinch bug);  Blostomatidae  spp.;  Brevicoryne brassicae  Linnaeus (cabbage aphid);  Cacopsylla pyricola  Foerster (pear psylla);  Calocoris norvegicus  Gmelin (potato capsid bug);  Chaetosiphon fragaefolii  Cockerell (strawberry aphid);  Cimicidae  spp.;  Coreidae  spp.;  Corythuca gossypii  Fabricius (cotton lace bug);  Cyrtopeltis modesta  Distant (tomato bug);  C. notatus  Distant (suckfly);  Deois flavopicta  St l (spittlebug);  Dialeurodes citri  Ashmead (citrus whitefly);  Diaphnocoris chlorionis  Say (honeylocust plant bug);  Diuraphis noxia  Kurdjumov/Mordvilko (Russian wheat aphid);  Duplachionaspis divergens  Green (armored scale);  Dysaphis plantaginea  Paaserini (rosy apple aphid);  Dysdercus suturellus  Herrich-Schäffer (cotton stainer);  Dysmicoccus boninsis  Kuwana (gray sugarcane mealybug);  Empoasca fabae  Harris (potato leafhopper);  Eriosoma lanigerum  Hausmann (woolly apple aphid);  Erythroneoura  spp. (grape leafhoppers);  Eumetopina flavipes  Muir (Island sugarcane planthopper);  Eurygaster  spp.;  Euschistus servus  Say (brown stink bug);  E. variolarius  Palisot de Beauvois (one-spotted stink bug);  Graptostethus  spp. (complex of seed bugs); and  Hyalopterus pruni  Geoffroy (mealy plum aphid);  Icerya purchasi  Maskell (cottony cushion scale);  Labopidicola allii  Knight (onion plant bug);  Laodelphax striatellus  Fallen (smaller brown planthopper);  Leptoglossus corculus  Say (leaf-footed pine seed bug);  Leptodictya tabida  Herrich-Schaeffer (sugarcane lace bug);  Lipaphis erysimi  Kaltenbach (turnip aphid);  Lygocoris pabulinus  Linnaeus (common green capsid);  Lygus lineolaris  Palisot de Beauvois (tarnished plant bug);  L. Hesperus  Knight (Western tarnished plant bug);  L. pratensis  Linnaeus (common meadow bug);  L. rugulipennis  Poppius (European tarnished plant bug);  Macrosiphum euphorbiae  Thomas (potato aphid);  Macrosteles quadrilineatus  Forbes (aster leafhopper);  Magicicada septendecim  Linnaeus (periodical cicada);  Mahanarva fimbriolata  St l (sugarcane spittlebug);  M. posticata  St l (little cicada of sugarcane);  Melanaphis sacchari  Zehntner (sugarcane aphid);  Melanaspis glomerata  Green (black scale);  Metopolophium dirhodum  Walker (rose grain aphid);  Myzus persicae  Sulzer (peach-potato aphid, green peach aphid);  Nasonovia ribisnigri  Mosley (lettuce aphid);  Nephotettix cinticeps  Uhler (green leafhopper);  N. nigropictus  St l (rice leafhopper);  Nezara viridula  Linnaeus (southern green stink bug);  Nilaparvata lugens  St l (brown planthopper);  Nysius ericae  Schilling (false chinch bug);  Nysius raphanus  Howard (false chinch bug);  Oebalus pugnax  Fabricius (rice stink bug);  Oncopeltus fasciatus  Dallas (large milkweed bug);  Orthops campestris  Linnaeus;  Pemphigus  spp. (root aphids and gall aphids);  Peregrinus maidis  Ashmead (corn planthopper);  Perkinsiella saccharicida  Kirkaldy (sugarcane delphacid);  Phylloxera devastatrix  Pergande (pecan phylloxera);  Planococcus citri  Risso (citrus mealybug);  Plesiocoris rugicollis  Fallen (apple capsid);  Poecilocapsus lineatus  Fabricius (four-lined plant bug);  Pseudatomoscelis seriatus  Reuter (cotton fleahopper);  Pseudococcus  spp. (other mealybug complex);  Pulvinaria elongata  Newstead (cottony grass scale);  Pyrilla perpusilla  Walker (sugarcane leafhopper);  Pyrrhocoridae  spp.;  Quadraspidiotus perniciosus  Comstock (San Jose scale);  Reduviidae  spp.;  Rhopalosiphum maidis  Fitch (corn leaf aphid);  R. padi  Linnaeus (bird cherry-oat aphid);  Saccharicoccus sacchari  Cockerell (pink sugarcane mealybug);  Scaptacoris castanea  Perty (brown root stink bug);  Schizaphis graminum  Rondani (greenbug);  Sipha flava  Forbes (yellow sugarcane aphid);  Sitobion avenae  Fabricius (English grain aphid);  Sogatella furcifera  Horvath (white-backed planthopper);  Sogatodes oryzicola  Muir (rice delphacid);  Spanagonicus albofasciatus  Reuter (whitemarked fleahopper);  Therioaphis maculata  Buckton (spotted alfalfa aphid);  Tinidae  spp.;  Toxoptera aurantii  Boyer de Fonscolombe (black citrus aphid); and  T. citricida  Kirkaldy (brown citrus aphid);  Trialeurodes abutiloneus  (bandedwinged whitefly) and  T. vaporariorum  Westwood (greenhouse whitefly);  Trioza diospyri  Ashmead (persimmon psylla); and  Typhlocyba pomaria  McAtee (white apple leafhopper). 
     Also included are adults and larvae of the order Acari (mites) such as  Aceria tosichella  Keifer (wheat curl mite);  Panonychus ulmi  Koch (European red mite);  Petrobia latens  Muller (brown wheat mite);  Steneotarsonemus bancrofti  Michael (sugarcane stalk mite); spider mites and red mites in the family Tetranychidae,  Oligonychus grypus  Baker &amp; Pritchard,  O. indicus  Hirst (sugarcane leaf mite),  O. pratensis  Banks (Banks grass mite),  O. stickneyi  McGregor (sugarcane spider mite);  Tetranychus urticae  Koch (two spotted spider mite);  T. mcdanieli  McGregor (McDaniel mite);  T. cinnabarinus  Boisduval (carmine spider mite);  T. turkestani  Ugarov &amp; Nikolski (strawberry spider mite), flat mites in the family Tenuipalpidae,  Brevipalpus lewisi  McGregor (citrus flat mite); rust and bud mites in the family Eriophyidae and other foliar feeding mites and mites important in human and animal health, i.e. dust mites in the family Epidermoptidae, follicle mites in the family Demodicidae, grain mites in the family Glycyphagidae, ticks in the order Ixodidae.  Ixodes scapularis  Say (deer tick);  I. holocyclus  Neumann (Australian paralysis tick);  Dermacentor variabilis  Say (American dog tick);  Amblyomma americanum  Linnaeus (lone star tick); and scab and itch mites in the families Psoroptidae, Pyemotidae, and Sarcoptidae. 
     Insect pests of the order Thysanura are of interest, such as  Lepisma saccharina  Linnaeus (silverfish);  Thermobia domestica  Packard (firebrat). 
     Additional arthropod pests covered include: spiders in the order Araneae such as  Loxosceles reclusa  Gertsch &amp; Mulaik (brown recluse spider); and the  Latrodectus mactans  Fabricius (black widow spider); and centipedes in the order Scutigeromorpha such as  Scutigera coleoptrata  Linnaeus (house centipede). In addition, insect pests of the order Isoptera are of interest, including those of the termitidae family, such as, but not limited to,  Cornitermes cumulans  Kollar,  Cylindrotermes nordenskioeldi  Holmgren and  Pseudacanthotermes militaris  Hagen (sugarcane termite); as well as those in the Rhinotermitidae family including, but not limited to  Heterotermes tenuis  Hagen. Insects of the order Thysanoptera are also of interest, including but not limited to thrips, such as  Stenchaetothrips minutus  van Deventer (sugarcane thrips). 
     The insect pests can be adults, larvae or even ova. A preferred developmental stage for testing for pesticidal activity is larvae or other immature form of the insect pest. Methods of rearing insect larvae and performing bioassays are well known in the art. See, e.g., Czapla &amp; Lang (1990)  J. Econ. Entomol.  83:2480-2485; Griffith &amp; Smith (1977)  J. Aust. Ent. Soc.  16:366; Keiper &amp; Foote (1996)  Hydrobiologia  339:137-139; and U.S. Pat. No. 5,351,643. For example, insect pests can be reared in total darkness at about 20° C. to about 30° C. and from about 30% to about 70% relative humidity. 
     The novel chimeric pesticidal polypeptides can exhibit improved pesticidal activity when compared to a pesticidal polypeptide lacking a solubility-enhancing polypeptide. As used herein, the term “improved pesticidal activity” refers to a polypeptide that has enhanced pesticidal activity following the presently disclosed methods relative to the activity of the corresponding pesticidal polypeptide lacking a solubility-enhancing polypeptide as made by the methods disclosed herein and/or to a polypeptide that is effective against a broader range of pests, and/or a polypeptide having specificity for a pest that is not susceptible to the toxicity of the polypeptide prior to modification of its sequence using the presently disclosed methods. A finding of improved or enhanced pesticidal activity requires a demonstration of an increase of pesticidal activity of at least 10%, against the pest target or at least 20%, 25%, 30%, 35%, 40%, 45%, 50%, 60%, 70%, 100%, 150%, 200% or 300% or greater increase of pesticidal activity relative to the pesticidal activity of the polypeptide prior to the addition of a solubility-enhancing polypeptide, as determined against the same pest. 
     For example, an improved pesticidal activity is provided where a wider or narrower range of pests is impacted by the polypeptide relative to the range of pests that is affected by the polypeptide prior to sequence modification. A wider range of impact may be desirable where versatility is desired, while a narrower range of impact may be desirable where, for example, beneficial insects might otherwise be impacted by use or presence of the pesticidal polypeptide. While the invention is not bound by any particular mechanism of action, an improved pesticidal activity may also be provided by changes in one or more characteristics of a polypeptide; for example, the stability or longevity of a polypeptide in an insect gut may be increased relative to the stability or longevity of a corresponding wild-type protein. 
     A “subject plant or plant cell” is one in which genetic alteration, such as transformation, has been affected as to a gene of interest or is a plant or plant cell which is descended from a plant or cell so altered and which comprises the alteration. A “control” or “control plant” or “control plant cell” provides a reference point for measuring changes in phenotype of the subject plant or plant cell. 
     A control plant or plant cell may comprise, for example: (a) a wild-type plant or cell, i.e., of the same genotype as the starting material for the genetic alteration which resulted in the subject plant or cell; (b) a plant or plant cell of the same genotype as the starting material but which has been transformed with a null construct (i.e. with a construct which has no known effect on the trait of interest, such as a construct comprising a marker gene); (c) a plant or plant cell which is a non-transformed segregant among progeny of a subject plant or plant cell; (d) a plant or plant cell genetically identical to the subject plant or plant cell but which is not exposed to conditions or stimuli that would induce expression of the gene of interest; or (e) the subject plant or plant cell itself, under conditions in which the gene of interest is not expressed. 
     As noted above, the methods involve generating novel chimeric pesticidal polypeptide sequences and the nucleotide sequences that encode such polypeptides. The novel chimeric pesticidal polypeptide sequences are produced by making a fusion protein comprising an amino acid sequence of a solubility-enhancing polypeptide operably linked to an amino acid sequence of a pesticidal protein, particularly a cry protein. 
     Any pesticidal protein can be used in the presently disclosed methods. In some embodiments, the pesticidal protein is a δ-endotoxin of  Bacillus  spp. The specific activity of δ-endotoxins is considered highly beneficial. Unlike most insecticides, the δ-endotoxins do not have a broad spectrum of activity, so they typically do not kill beneficial insects. Furthermore, the δ-endotoxins are non-toxic to mammals, including humans, domesticated animals, and wildlife. In particular embodiments, the δ-endotoxin is a Cry protein. 
     It is well known that naturally occurring δ-endotoxins are synthesized by  B. thuringiensis  sporulating cells as a proteinaceous crystalline inclusion protoxin. Upon being ingested by susceptible insect larvae, the microcrystals dissolve in the midgut, and the protoxin is transformed into a biologically active moiety by proteases characteristic of digestive enzymes located in the insect gut. The activated δ-endotoxin binds with high affinity to protein receptors on brush-border membrane vesicles. The epithelial cells lining the midgut are the primary target of the endotoxin and are rapidly destroyed as a consequence of membrane perforation resulting from the formation of gated, cation-selective channels by the toxin. Both Cry and Cyt toxins are pore-forming toxins. The α-helix regions of the Cry toxins form the trans-membrane pore, whereas Cyt toxins insert into the membrane by forming a β-barrel comprised of β-sheets from each monomer. 
     Bt Cry proteins have five conserved sequence domains, and three conserved structural domains (see, e.g., de Maagd et al. (2001)  Trends Genetics  17:193-199). The most amino-terminal conserved structural domain (Domain I) consists of seven alpha helices, with a central hydrophobic helix-α5 encircled by six other amphipathic helices, and is involved in membrane insertion and pore formation. The second conserved structural domain (Domain II) consists of three antiparallel beta-sheets implicated in cell binding, and the most carboxy-terminal conserved structural domain (Domain III) consists of a beta-sandwich. Exposed regions in domains II and III are involved in receptor recognition and binding, and are therefore considered determinants of toxin specificity. The location and properties of these domains are known to those of skill in the art. See, for example, Grochulski et al. (1995)  J Mol Biol  254:447-464; Morse, Yamamoto, and Stroud (2001)  Structure  9:409-417; Li et al. (1991)  Nature  353:815-821; Galitsky et al. (2001)  Acta Cryst  D57:1101-1109; Boonserm et al. (2006)  J Bacteriol  188:3391-3401; Boonserm et al. (2005)  J Mol Biol  348:363-382; and Guo et al. (2009)  J Struct Biol  168:259-266. 
     Bt Cyt proteins have a single α-β domain comprising two outer layers of α-helix hairpins wrapped around a β-sheet (Li, Koni, and Ellar (1996)  J Mol Biol  257:129-152; and Cohen et al. (2008)  J Mol Biol  380:820-827). The β-sheet is involved in membrane insertion. 
     A list of some known δ-endotoxins (Cry and Cyt endotoxins) and their GenBank Accession Nos. are listed in Table 2, which can be used as a source for nucleic and amino acid sequences for use in the methods disclosed herein. 
     
       
         
           
               
               
               
               
               
             
               
                   
                 TABLE 2 
               
               
                   
                   
               
               
                   
                 Endotoxin 
                 Accession # 
                 Endotoxin 
                 Accession # 
               
               
                   
                   
               
             
            
               
                   
                 Cry1Aa1 
                 AAA22353 
                 Cry1Aa2 
                 AAA22552 
               
               
                   
                 Cry1Aa3 
                 BAA00257 
                 Cry1Aa4 
                 CAA31886 
               
               
                   
                 Cry1Aa5 
                 BAA04468 
                 Cry1Aa6 
                 AAA86265 
               
               
                   
                 Cry1Aa7 
                 AAD46139 
                 Cry1Aa8 
                 I26149 
               
               
                   
                 Cry1Aa9 
                 BAA77213 
                 Cry1Aa10 
                 AAD55382 
               
               
                   
                 Cry1Aa11 
                 CAA70856 
                 Cry1Aa12 
                 AAP80146 
               
               
                   
                 Cry1Aa13 
                 AAM44305 
                 Cry1Aa14 
                 AAP40639 
               
               
                   
                 Cry1Aa15 
                 AAY66993 
                 Cry1Aa16 
                 HQ439776 
               
               
                   
                 Cry1Aa17 
                 HQ439788 
                 Cry1Aa18 
                 HQ439790 
               
               
                   
                 Cry1Aa19 
                 HQ685121 
                 Cry1Aa20 
                 JF340156 
               
               
                   
                 Cry1Aa21 
                 JN651496 
                 Cry1Aa22 
                 KC158223 
               
               
                   
                 Cry1Ab1 
                 AAA22330 
                 Cry1Ab2 
                 AAA22613 
               
               
                   
                 Cry1Ab3 
                 AAA22561 
                 Cry1Ab4 
                 BAA00071 
               
               
                   
                 Cry1Ab5 
                 CAA28405 
                 Cry1Ab6 
                 AAA22420 
               
               
                   
                 Cry1Ab7 
                 CAA31620 
                 Cry1Ab8 
                 AAA22551 
               
               
                   
                 Cry1Ab9 
                 CAA38701 
                 Cry1Ab10 
                 A29125 
               
               
                   
                 Cry1Ab11 
                 I12419 
                 Cry1Ab12 
                 AAC64003 
               
               
                   
                 Cry1Ab13 
                 AAN76494 
                 Cry1Ab14 
                 AAG16877 
               
               
                   
                 Cry1Ab15 
                 AAO13302 
                 Cry1Ab16 
                 AAK55546 
               
               
                   
                 Cry1Ab17 
                 AAT46415 
                 Cry1Ab18 
                 AAQ88259 
               
               
                   
                 Cry1Ab19 
                 AAW31761 
                 Cry1Ab20 
                 ABB72460 
               
               
                   
                 Cry1Ab21 
                 ABS18384 
                 Cry1Ab22 
                 ABW87320 
               
               
                   
                 Cry1Ab23 
                 HQ439777 
                 Cry1Ab24 
                 HQ439778 
               
               
                   
                 Cry1Ab25 
                 HQ685122 
                 Cry1Ab26 
                 HQ847729 
               
               
                   
                 Cry1Ab27 
                 JN135249 
                 Cry1Ab28 
                 JN135250 
               
               
                   
                 Cry1Ab29 
                 JN135251 
                 Cry1Ab30 
                 JN135252 
               
               
                   
                 Cry1Ab31 
                 JN135253 
                 Cry1Ab32 
                 JN135254 
               
               
                   
                 Cry1Ab33 
                 AAS93798 
                 Cry1Ab34 
                 KC156668 
               
               
                   
                 Cry1Ab-like 
                 AAK14336 
                 Cry1Ab-like 
                 AAK14337 
               
               
                   
                 Cry1Ab-like 
                 AAK14338 
                 Cry1Ab-like 
                 ABG88858 
               
               
                   
                 Cry1Ac1 
                 AAA22331 
                 Cry1Ac2 
                 AAA22338 
               
               
                   
                 Cry1Ac3 
                 CAA38098 
                 Cry1Ac4 
                 Cry1Ac4 
               
               
                   
                 Cry1Ac5 
                 AAA22339 
                 Cry1Ac6 
                 AAA86266 
               
               
                   
                 Cry1Ac7 
                 AAB46989 
                 Cry1Ac8 
                 AAC44841 
               
               
                   
                 Cry1Ac9 
                 AAB49768 
                 Cry1Ac10 
                 CAA05505 
               
               
                   
                 Cry1Ac11 
                 CAA10270 
                 Cry1Ac12 
                 I12418 
               
               
                   
                 Cry1Ac13 
                 AAD38701 
                 Cry1Ac14 
                 AAQ06607 
               
               
                   
                 Cry1Ac15 
                 AAN07788 
                 Cry1Ac16 
                 AAU87037 
               
               
                   
                 Cry1Ac17 
                 AAX18704 
                 Cry1Ac18 
                 AAY88347 
               
               
                   
                 Cry1Ac19 
                 ABD37053 
                 Cry1Ac20 
                 ABB89046 
               
               
                   
                 Cry1Ac21 
                 AAY66992 
                 Cry1Ac22 
                 ABZ01836 
               
               
                   
                 Cry1Ac23 
                 CAQ30431 
                 Cry1Ac24 
                 ABL01535 
               
               
                   
                 Cry1Ac25 
                 FJ513324 
                 Cry1Ac26 
                 FJ617446 
               
               
                   
                 Cry1Ac27 
                 FJ617447 
                 Cry1Ac28 
                 ACM90319 
               
               
                   
                 Cry1Ac29 
                 DQ438941 
                 Cry1Ac30 
                 GQ227507 
               
               
                   
                 Cry1Ac31 
                 GU446674 
                 Cry1Ac32 
                 HM061081 
               
               
                   
                 Cry1Ac33 
                 GQ866913 
                 Cry1Ac34 
                 HQ230364 
               
               
                   
                 Cry1Ac35 
                 JF340157 
                 Cry1Ac36 
                 JN387137 
               
               
                   
                 Cry1Ac37 
                 JQ317685 
                 Cry1Ad1 
                 AAA22340 
               
               
                   
                 Cry1Ad2 
                 CAA01880 
                 Cry1Ae1 
                 AAA22410 
               
               
                   
                 Cry1Af1 
                 AAB82749 
                 Cry1Ag1 
                 AAD46137 
               
               
                   
                 Cry1Ah1 
                 AAQ14326 
                 Cry1Ah2 
                 ABB76664 
               
               
                   
                 Cry1Ah3 
                 HQ439779 
                 Cry1Ai1 
                 AAO39719 
               
               
                   
                 Cry1Ai2 
                 HQ439780 
                 Cry1A-like 
                 AAK14339 
               
               
                   
                 Cry1Ba1 
                 CAA29898 
                 Cry1Ba2 
                 CAA65003 
               
               
                   
                 Cry1Ba3 
                 AAK63251 
                 Cry1Ba4 
                 AAK51084 
               
               
                   
                 Cry1Ba5 
                 AB020894 
                 Cry1Ba6 
                 ABL60921 
               
               
                   
                 Cry1Ba7 
                 HQ439781 
                 Cry1Bb1 
                 AAA22344 
               
               
                   
                 Cry1Bb2 
                 HQ439782 
                 Cry1Bc1 
                 CAA86568 
               
               
                   
                 Cry1Bd1 
                 AAD10292 
                 Cry1Bd2 
                 AAM93496 
               
               
                   
                 Cry1Be1 
                 AAC32850 
                 Cry1Be2 
                 AAQ52387 
               
               
                   
                 Cry1Be3 
                 ACV96720 
                 Cry1Be4 
                 HM070026 
               
               
                   
                 Cry1Bf1 
                 CAC50778 
                 Cry1Bf2 
                 AAQ52380 
               
               
                   
                 Cry1Bg1 
                 AAO39720 
                 Cry1Bh1 
                 HQ589331 
               
               
                   
                 Cry1Bi1 
                 KC156700 
                 Cry1Ca1 
                 CAA30396 
               
               
                   
                 Cry1Ca2 
                 CAA31951 
                 Cry1Ca3 
                 AAA22343 
               
               
                   
                 Cry1Ca4 
                 CAA01886 
                 Cry1Ca5 
                 CAA65457 
               
               
                   
                 Cry1Ca6 
                 AAF37224 
                 Cry1Ca7 
                 AAG50438 
               
               
                   
                 Cry1Ca8 
                 AAM00264 
                 Cry1Ca9 
                 AAL79362 
               
               
                   
                 Cry1Ca10 
                 AAN16462 
                 Cry1Ca11 
                 AAX53094 
               
               
                   
                 Cry1Ca12 
                 HM070027 
                 Cry1Ca13 
                 HQ412621 
               
               
                   
                 Cry1Ca14 
                 JN651493 
                 Cry1Cb1 
                 M97880 
               
               
                   
                 Cry1Cb2 
                 AAG35409 
                 Cry1Cb3 
                 ACD50894 
               
               
                   
                 Cry1Cb-like 
                 AAX63901 
                 Cry1Da1 
                 CAA38099 
               
               
                   
                 Cry1Da2 
                 I76415 
                 Cry1Da3 
                 HQ439784 
               
               
                   
                 Cry1Db1 
                 CAA80234 
                 Cry1Db2 
                 AAK48937 
               
               
                   
                 Cry1Dc1 
                 ABK35074 
                 Cry1Ea1 
                 CAA37933 
               
               
                   
                 Cry1Ea2 
                 CAA39609 
                 Cry1Ea3 
                 AAA22345 
               
               
                   
                 Cry1Ea4 
                 AAD04732 
                 Cry1Ea5 
                 A15535 
               
               
                   
                 Cry1Ea6 
                 AAL50330 
                 Cry1Ea7 
                 AAW72936 
               
               
                   
                 Cry1Ea8 
                 ABX11258 
                 Cry1Ea9 
                 HQ439785 
               
               
                   
                 Cry1Ea10 
                 ADR00398 
                 Cry1Ea11 
                 JQ652456 
               
               
                   
                 Cry1Eb1 
                 AAA22346 
                 Cry1Fa1 
                 AAA22348 
               
               
                   
                 Cry1Fa2 
                 AAA22347 
                 Cry1Fa3 
                 HM070028 
               
               
                   
                 Cry1Fa4 
                 HM439638 
                 Cry1Fb1 
                 CAA80235 
               
               
                   
                 Cry1Fb2 
                 BAA25298 
                 Cry1Fb3 
                 AAF21767 
               
               
                   
                 Cry1Fb4 
                 AAC10641 
                 Cry1Fb5 
                 AAO13295 
               
               
                   
                 Cry1Fb6 
                 ACD50892 
                 Cry1Fb7 
                 ACD50893 
               
               
                   
                 Cry1Ga1 
                 CAA80233 
                 Cry1Ga2 
                 CAA70506 
               
               
                   
                 Cry1Gb1 
                 AAD10291 
                 Cry1Gb2 
                 AAO13756 
               
               
                   
                 Cry1Gc1 
                 AAQ52381 
                 Cry1Ha1 
                 CAA80236 
               
               
                   
                 Cry1Hb1 
                 AAA79694 
                 Cry1Hb2 
                 HQ439786 
               
               
                   
                 Cry1H-like 
                 AAF01213 
                 Cry1Ia1 
                 CAA44633 
               
               
                   
                 Cry1Ia2 
                 AAA22354 
                 Cry1Ia3 
                 AAC36999 
               
               
                   
                 Cry1Ia4 
                 AAB00958 
                 Cry1Ia5 
                 CAA70124 
               
               
                   
                 Cry1Ia6 
                 AAC26910 
                 Cry1Ia7 
                 AAM73516 
               
               
                   
                 Cry1Ia8 
                 AAK66742 
                 Cry1Ia9 
                 AAQ08616 
               
               
                   
                 Cry1Ia10 
                 AAP86782 
                 Cry1Ia11 
                 CAC85964 
               
               
                   
                 Cry1Ia12 
                 AAV53390 
                 Cry1Ia13 
                 ABF83202 
               
               
                   
                 Cry1Ia14 
                 ACG63871 
                 Cry1Ia15 
                 FJ617445 
               
               
                   
                 Cry1Ia16 
                 FJ617448 
                 Cry1Ia17 
                 GU989199 
               
               
                   
                 Cry1Ia18 
                 ADK23801 
                 Cry1Ia19 
                 HQ439787 
               
               
                   
                 Cry1Ia20 
                 JQ228426 
                 Cry1Ia21 
                 JQ228424 
               
               
                   
                 Cry1Ia22 
                 JQ228427 
                 Cry1Ia23 
                 JQ228428 
               
               
                   
                 Cry1Ia24 
                 JQ228429 
                 Cry1Ia25 
                 JQ228430 
               
               
                   
                 Cry1Ia26 
                 JQ228431 
                 Cry1Ia27 
                 JQ228432 
               
               
                   
                 Cry1Ia28 
                 JQ228433 
                 Cry1Ia29 
                 JQ228434 
               
               
                   
                 Cry1Ia30 
                 JQ317686 
                 Cry1Ia31 
                 JX944038 
               
               
                   
                 Cry1Ia32 
                 JX944039 
                 Cry1Ia33 
                 JX944040 
               
               
                   
                 Cry1Ib1 
                 AAA82114 
                 Cry1Ib2 
                 ABW88019 
               
               
                   
                 Cry1Ib3 
                 ACD75515 
                 Cry1Ib4 
                 HM051227 
               
               
                   
                 Cry1Ib5 
                 HM070028 
                 Cry1Ib6 
                 ADK38579 
               
               
                   
                 Cry1Ib7 
                 JN571740 
                 Cry1Ib8 
                 JN675714 
               
               
                   
                 Cry1Ib9 
                 JN675715 
                 Cry1Ib10 
                 JN675716 
               
               
                   
                 Cry1Ib11 
                 JQ228423 
                 Cry1Ic1 
                 AAC62933 
               
               
                   
                 Cry1Ic2 
                 AAE71691 
                 Cry1Id1 
                 AAD44366 
               
               
                   
                 Cry1Id2 
                 JQ228422 
                 Cry1Ie1 
                 AAG43526 
               
               
                   
                 Cry1Ie2 
                 HM439636 
                 Cry1Ie3 
                 KC156647 
               
               
                   
                 Cry1Ie4 
                 KC156681 
                 Cry1If1 
                 AAQ52382 
               
               
                   
                 Cry1Ig1 
                 KC156701 
                 Cry1I-like 
                 AAC31094 
               
               
                   
                 Cry1I-like 
                 ABG88859 
                 Cry1Ja1 
                 AAA22341 
               
               
                   
                 Cry1Ja2 
                 HM070030 
                 Cry1Ja3 
                 JQ228425 
               
               
                   
                 Cry1Jb1 
                 AAA98959 
                 Cry1Jc1 
                 AAC31092 
               
               
                   
                 Cry1Jc2 
                 AAQ52372 
                 Cry1Jd1 
                 CAC50779 
               
               
                   
                 Cry1Ka1 
                 AAB00376 
                 Cry1Ka2 
                 HQ439783 
               
               
                   
                 Cry1La1 
                 AAS60191 
                 Cry1La2 
                 HM070031 
               
               
                   
                 Cry1Ma1 
                 FJ884067 
                 Cry1Ma2 
                 KC156659 
               
               
                   
                 Cry1Na1 
                 KC156648 
                 Cry1Nb1 
                 KC156678 
               
               
                   
                 Cry1-like 
                 AAC31091 
                 Cry2Aa1 
                 AAA22335 
               
               
                   
                 Cry2Aa2 
                 AAA83516 
                 Cry2Aa3 
                 D86064 
               
               
                   
                 Cry2Aa4 
                 AAC04867 
                 Cry2Aa5 
                 CAA10671 
               
               
                   
                 Cry2Aa6 
                 CAA10672 
                 Cry2Aa7 
                 CAA10670 
               
               
                   
                 Cry2Aa8 
                 AA013734 
                 Cry2Aa9 
                 AAO13750 
               
               
                   
                 Cry2Aa10 
                 AAQ04263 
                 Cry2Aa11 
                 AAQ52384 
               
               
                   
                 Cry2Aa12 
                 ABI83671 
                 Cry2Aa13 
                 ABL01536 
               
               
                   
                 Cry2Aa14 
                 ACF04939 
                 Cry2Aa15 
                 JN426947 
               
               
                   
                 Cry2Ab1 
                 AAA22342 
                 Cry2Ab2 
                 CAA39075 
               
               
                   
                 Cry2Ab3 
                 AAG36762 
                 Cry2Ab4 
                 AAO13296 
               
               
                   
                 Cry2Ab5 
                 AAQ04609 
                 Cry2Ab6 
                 AAP59457 
               
               
                   
                 Cry2Ab7 
                 AAZ66347 
                 Cry2Ab8 
                 ABC95996 
               
               
                   
                 Cry2Ab9 
                 ABC74968 
                 Cry2Ab10 
                 EF157306 
               
               
                   
                 Cry2Ab11 
                 CAM84575 
                 Cry2Ab12 
                 ABM21764 
               
               
                   
                 Cry2Ab13 
                 ACG76120 
                 Cry2Ab14 
                 ACG76121 
               
               
                   
                 Cry2Ab15 
                 HM037126 
                 Cry2Ab16 
                 GQ866914 
               
               
                   
                 Cry2Ab17 
                 HQ439789 
                 Cry2Ab18 
                 JN135255 
               
               
                   
                 Cry2Ab19 
                 JN135256 
                 Cry2Ab20 
                 JN135257 
               
               
                   
                 Cry2Ab21 
                 JN135258 
                 Cry2Ab22 
                 JN135259 
               
               
                   
                 Cry2Ab23 
                 JN135260 
                 Cry2Ab24 
                 JN135261 
               
               
                   
                 Cry2Ab25 
                 JN415485 
                 Cry2Ab26 
                 JN426946 
               
               
                   
                 Cry2Ab27 
                 JN415764 
                 Cry2Ab28 
                 JN651494 
               
               
                   
                 Cry2Ac1 
                 CAA40536 
                 Cry2Ac2 
                 AAG35410 
               
               
                   
                 Cry2Ac3 
                 AAQ52385 
                 Cry2Ac4 
                 ABC95997 
               
               
                   
                 Cry2Ac5 
                 ABC74969 
                 Cry2Ac6 
                 ABC74793 
               
               
                   
                 Cry2Ac7 
                 CAL18690 
                 Cry2Ac8 
                 CAM09325 
               
               
                   
                 Cry2Ac9 
                 CAM09326 
                 Cry2Ac10 
                 ABN15104 
               
               
                   
                 Cry2Ac11 
                 CAM83895 
                 Cry2Ac12 
                 CAM83896 
               
               
                   
                 Cry2Ad1 
                 AAF09583 
                 Cry2Ad2 
                 ABC86927 
               
               
                   
                 Cry2Ad3 
                 CAK29504 
                 Cry2Ad4 
                 CAM32331 
               
               
                   
                 Cry2Ad5 
                 CAO78739 
                 Cry2Ae1 
                 AAQ52362 
               
               
                   
                 Cry2Af1 
                 ABO30519 
                 Cry2Af2 
                 GQ866915 
               
               
                   
                 Cry2Ag1 
                 ACH91610 
                 Cry2Ah1 
                 EU939453 
               
               
                   
                 Cry2Ah2 
                 ACL80665 
                 Cry2Ah3 
                 GU073380 
               
               
                   
                 Cry2Ah4 
                 KC156702 
                 Cry2Ai1 
                 FJ788388 
               
               
                   
                 Cry2Aj 
                   
                 Cry2Ak1 
                 KC156660 
               
               
                   
                 Cry2Ba1 
                 KC156658 
                 Cry3Aa1 
                 AAA22336 
               
               
                   
                 Cry3Aa2 
                 AAA22541 
                 Cry3Aa3 
                 CAA68482 
               
               
                   
                 Cry3Aa4 
                 AAA22542 
                 Cry3Aa5 
                 AAA50255 
               
               
                   
                 Cry3Aa6 
                 AAC43266 
                 Cry3Aa7 
                 CAB41411 
               
               
                   
                 Cry3Aa8 
                 AAS79487 
                 Cry3Aa9 
                 AAW05659 
               
               
                   
                 Cry3Aa10 
                 AAU29411 
                 Cry3Aa11 
                 AAW82872 
               
               
                   
                 Cry3Aa12 
                 ABY49136 
                 Cry3Ba1 
                 CAA34983 
               
               
                   
                 Cry3Ba2 
                 CAA00645 
                 Cry3Ba3 
                 JQ397327 
               
               
                   
                 Cry3Bb1 
                 AAA22334 
                 Cry3Bb2 
                 AAA74198 
               
               
                   
                 Cry3Bb3 
                 I15475 
                 Cry3Ca1 
                 CAA42469 
               
               
                   
                 Cry4Aa1 
                 CAA68485 
                 Cry4Aa2 
                 BAA00179 
               
               
                   
                 Cry4Aa3 
                 CAD30148 
                 Cry4Aa4 
                 AFB18317 
               
               
                   
                 Cry4A-like 
                 AAY96321 
                 Cry4Ba1 
                 CAA30312 
               
               
                   
                 Cry4Ba2 
                 CAA30114 
                 Cry4Ba3 
                 AAA22337 
               
               
                   
                 Cry4Ba4 
                 BAA00178 
                 Cry4Ba5 
                 CAD30095 
               
               
                   
                 Cry4Ba-like 
                 ABC47686 
                 Cry4Ca1 
                 EU646202 
               
               
                   
                 Cry4Cb1 
                 FJ403208 
                 Cry4Cb2 
                 FJ597622 
               
               
                   
                 Cry4Cc1 
                 FJ403207 
                 Cry5Aa1 
                 AAA67694 
               
               
                   
                 Cry5Ab1 
                 AAA67693 
                 Cry5Ac1 
                 I34543 
               
               
                   
                 Cry5Ad1 
                 ABQ82087 
                 Cry5Ba1 
                 AAA68598 
               
               
                   
                 Cry5Ba2 
                 ABW88931 
                 Cry5Ba3 
                 AFJ04417 
               
               
                   
                 Cry5Ca1 
                 HM461869 
                 Cry5Ca2 
                 ZP_04123426 
               
               
                   
                 Cry5Da1 
                 HM461870 
                 Cry5Da2 
                 ZP_04123980 
               
               
                   
                 Cry5Ea1 
                 HM485580 
                 Cry5Ea2 
                 ZP_04124038 
               
               
                   
                 Cry6Aa1 
                 AAA22357 
                 Cry6Aa2 
                 AAM46849 
               
               
                   
                 Cry6Aa3 
                 ABH03377 
                 Cry6Ba1 
                 AAA22358 
               
               
                   
                 Cry7Aa1 
                 AAA22351 
                 Cry7Ab1 
                 AAA21120 
               
               
                   
                 Cry7Ab2 
                 AAA21121 
                 Cry7Ab3 
                 ABX24522 
               
               
                   
                 Cry7Ab4 
                 EU380678 
                 Cry7Ab5 
                 ABX79555 
               
               
                   
                 Cry7Ab6 
                 AC144005 
                 Cry7Ab7 
                 ADB89216 
               
               
                   
                 Cry7Ab8 
                 GU145299 
                 Cry7Ab9 
                 ADD92572 
               
               
                   
                 Cry7Ba1 
                 ABB70817 
                 Cry7Bb1 
                 KC156653 
               
               
                   
                 Cry7Ca1 
                 ABR67863 
                 Cry7Cb1 
                 KC156698 
               
               
                   
                 Cry7Da1 
                 ACQ99547 
                 Cry7Da2 
                 HM572236 
               
               
                   
                 Cry7Da3 
                 KC156679 
                 Cry7Ea1 
                 HM035086 
               
               
                   
                 Cry7Ea2 
                 HM132124 
                 Cry7Ea3 
                 EEM19403 
               
               
                   
                 Cry7Fa1 
                 HM035088 
                 Cry7Fa2 
                 EEM19090 
               
               
                   
                 Cry7Fb1 
                 HM572235 
                 Cry7Fb2 
                 KC156682 
               
               
                   
                 Cry7Ga1 
                 HM572237 
                 Cry7Ga2 
                 KC156669 
               
               
                   
                 Cry7Gb1 
                 KC156650 
                 Cry7Gc1 
                 KC156654 
               
               
                   
                 Cry7Gd1 
                 KC156697 
                 Cry7Ha1 
                 KC156651 
               
               
                   
                 Cry71a1 
                 KC156665 
                 Cry7Ja1 
                 KC156671 
               
               
                   
                 Cry7Ka1 
                 KC156680 
                 Cry7Kb1 
                 BAM99306 
               
               
                   
                 Cry7La1 
                 BAM99307 
                 Cry8Aa1 
                 AAA21117 
               
               
                   
                 Cry8Ab1 
                 EU044830 
                 Cry8Ac1 
                 KC156662 
               
               
                   
                 Cry8Ad1 
                 KC156684 
                 Cry8Ba1 
                 AAA21118 
               
               
                   
                 Cry8Bb1 
                 CAD57542 
                 Cry8Bc1 
                 CAD57543 
               
               
                   
                 Cry8Ca1 
                 AAA21119 
                 Cry8Ca2 
                 AAR98783 
               
               
                   
                 Cry8Ca3 
                 EU625349 
                 Cry8Ca4 
                 ADB54826 
               
               
                   
                 Cry8Da1 
                 BAC07226 
                 Cry8Da2 
                 BD133574 
               
               
                   
                 Cry8Da3 
                 BD133575 
                 Cry8Db1 
                 BAF93483 
               
               
                   
                 Cry8Ea1 
                 AAQ73470 
                 Cry8Ea2 
                 EU047597 
               
               
                   
                 Cry8Ea3 
                 KC855216 
                 Cry8Fa1 
                 AAT48690 
               
               
                   
                 Cry8Fa2 
                 HQ174208 
                 Cry8Fa3 
                 AFH78109 
               
               
                   
                 Cry8Ga1 
                 AAT46073 
                 Cry8Ga2 
                 ABC42043 
               
               
                   
                 Cry8Ga3 
                 FJ198072 
                 Cry8Ha1 
                 AAW81032 
               
               
                   
                 Cry81a1 
                 EU381044 
                 Cry81a2 
                 GU073381 
               
               
                   
                 Cry81a3 
                 HM044664 
                 Cry81a4 
                 KC156674 
               
               
                   
                 Cry81b1 
                 GU325772 
                 Cry81b2 
                 KC156677 
               
               
                   
                 Cry8Ja1 
                 EU625348 
                 Cry8Ka1 
                 FJ422558 
               
               
                   
                 Cry8Ka2 
                 ACN87262 
                 Cry8Kb1 
                 HM123758 
               
               
                   
                 Cry8Kb2 
                 KC156675 
                 Cry8La1 
                 GU325771 
               
               
                   
                 Cry8Ma1 
                 HM044665 
                 Cry8Ma2 
                 EEM86551 
               
               
                   
                 Cry8Ma3 
                 HM210574 
                 Cry8Na1 
                 HM640939 
               
               
                   
                 Cry8Pa1 
                 HQ388415 
                 Cry8Qa1 
                 HQ441166 
               
               
                   
                 Cry8Qa2 
                 KC152468 
                 Cry8Ra1 
                 AFP87548 
               
               
                   
                 Cry8Sa1 
                 JQ740599 
                 Cry8Ta1 
                 KC156673 
               
               
                   
                 Cry8-like 
                 FJ770571 
                 Cry8-like 
                 ABS53003 
               
               
                   
                 Cry9Aa1 
                 CAA41122 
                 Cry9Aa2 
                 CAA41425 
               
               
                   
                 Cry9Aa3 
                 GQ249293 
                 Cry9Aa4 
                 GQ249294 
               
               
                   
                 Cry9Aa5 
                 JX174110 
                 Cry9Aa like 
                 AAQ52376 
               
               
                   
                 Cry9Ba1 
                 CAA52927 
                 Cry9Ba2 
                 GU299522 
               
               
                   
                 Cry9Bb1 
                 AAV28716 
                 Cry9Ca1 
                 CAA85764 
               
               
                   
                 Cry9Ca2 
                 AAQ52375 
                 Cry9Da1 
                 BAA19948 
               
               
                   
                 Cry9Da2 
                 AAB97923 
                 Cry9Da3 
                 GQ249293 
               
               
                   
                 Cry9Da4 
                 GQ249297 
                 Cry9Db1 
                 AAX78439 
               
               
                   
                 Cry9Dc1 
                 KC156683 
                 Cry9Ea1 
                 BAA34908 
               
               
                   
                 Cry9Ea2 
                 AAO12908 
                 Cry9Ea3 
                 ABM21765 
               
               
                   
                 Cry9Ea4 
                 ACE88267 
                 Cry9Ea5 
                 ACF04743 
               
               
                   
                 Cry9Ea6 
                 ACG63872 
                 Cry9Ea7 
                 FJ380927 
               
               
                   
                 Cry9Ea8 
                 GQ249292 
                 Cry9Ea9 
                 JN651495 
               
               
                   
                 Cry9Eb1 
                 CAC50780 
                 Cry9Eb2 
                 GQ249298 
               
               
                   
                 Cry9Eb3 
                 KC156646 
                 Cry9Ec1 
                 AAC63366 
               
               
                   
                 Cry9Ed1 
                 AAX78440 
                 Cry9Ee1 
                 GQ249296 
               
               
                   
                 Cry9Ee2 
                 KC156664 
                 Cry9Fa1 
                 KC156692 
               
               
                   
                 Cry9Ga1 
                 KC156699 
                 Cry9-like 
                 AAC63366 
               
               
                   
                 Cry10Aa1 
                 AAA22614 
                 Cry10Aa2 
                 E00614 
               
               
                   
                 Cry10Aa3 
                 CAD30098 
                 Cry10Aa4 
                 AFB18318 
               
               
                   
                 Cry10A-like 
                 DQ167578 
                 Cry11Aa1 
                 AAA22352 
               
               
                   
                 Cry11Aa2 
                 AAA22611 
                 Cry11Aa3 
                 CAD30081 
               
               
                   
                 Cry11Aa4 
                 AFB18319 
                 Cry11Aa-like 
                 DQ166531 
               
               
                   
                 Cry11Ba1 
                 CAA60504 
                 Cry11Bb1 
                 AAC97162 
               
               
                   
                 Cry11Bb2 
                 HM068615 
                 Cry12Aa1 
                 AAA22355 
               
               
                   
                 Cry13Aa1 
                 AAA22356 
                 Cry14Aa1 
                 AAA21516 
               
               
                   
                 Cry14Ab1 
                 KC156652 
                 Cry15Aa1 
                 AAA22333 
               
               
                   
                 Cry16Aa1 
                 CAA63860 
                 Cry17Aa1 
                 CAA67841 
               
               
                   
                 Cry18Aa1 
                 CAA67506 
                 Cry18Ba1 
                 AAF89667 
               
               
                   
                 Cry18Ca1 
                 AAF89668 
                 Cry19Aa1 
                 CAA68875 
               
               
                   
                 Cry19Ba1 
                 BAA32397 
                 Cry19Ca1 
                 AFM37572 
               
               
                   
                 Cry20Aa1 
                 AAB93476 
                 Cry20Ba1 
                 ACS93601 
               
               
                   
                 Cry20Ba2 
                 KC156694 
                 Cry20-like 
                 GQ144333 
               
               
                   
                 Cry21Aa1 
                 I32932 
                 Cry21Aa2 
                 I66477 
               
               
                   
                 Cry21Ba1 
                 BAC06484 
                 Cry21Ca1 
                 JF521577 
               
               
                   
                 Cry21Ca2 
                 KC156687 
                 Cry21Da1 
                 JF521578 
               
               
                   
                 Cry22Aa1 
                 I34547 
                 Cry22Aa2 
                 CAD43579 
               
               
                   
                 Cry22Aa3 
                 ACD93211 
                 Cry22Ab1 
                 AAK50456 
               
               
                   
                 Cry22Ab2 
                 CAD43577 
                 Cry22Ba1 
                 CAD43578 
               
               
                   
                 Cry22Bb1 
                 KC156672 
                 Cry23Aa1 
                 AAF76375 
               
               
                   
                 Cry24Aa1 
                 AAC61891 
                 Cry24Ba1 
                 BAD32657 
               
               
                   
                 Cry24Ca1 
                 CAJ43600 
                 Cry25Aa1 
                 AAC61892 
               
               
                   
                 Cry26Aa1 
                 AAD25075 
                 Cry27Aa1 
                 BAA82796 
               
               
                   
                 Cry28Aa1 
                 AAD24189 
                 Cry28Aa2 
                 AAG00235 
               
               
                   
                 Cry29Aa1 
                 CAC80985 
                 Cry30Aa1 
                 CAC80986 
               
               
                   
                 Cry30Ba1 
                 BAD00052 
                 Cry30Ca1 
                 BAD67157 
               
               
                   
                 Cry30Ca2 
                 ACU24781 
                 Cry30Da1 
                 EF095955 
               
               
                   
                 Cry30Db1 
                 BAE80088 
                 Cry30Ea1 
                 ACC95445 
               
               
                   
                 Cry30Ea2 
                 FJ499389 
                 Cry30Fa1 
                 ACI22625 
               
               
                   
                 Cry30Ga1 
                 ACG60020 
                 Cry30Ga2 
                 HQ638217 
               
               
                   
                 Cry31Aa1 
                 BAB11757 
                 Cry31Aa2 
                 AAL87458 
               
               
                   
                 Cry31Aa3 
                 BAE79808 
                 Cry31Aa4 
                 BAF32571 
               
               
                   
                 Cry31Aa5 
                 BAF32572 
                 Cry31Aa6 
                 BAI44026 
               
               
                   
                 Cry31Ab1 
                 BAE79809 
                 Cry31Ab2 
                 BAF32570 
               
               
                   
                 Cry31Ac1 
                 BAF34368 
                 Cry31Ac2 
                 AB731600 
               
               
                   
                 Cry31Ad1 
                 BAI44022 
                 Cry32Aa1 
                 AAG36711 
               
               
                   
                 Cry32Aa2 
                 GU063849 
                 Cry32Ab1 
                 GU063850 
               
               
                   
                 Cry32Ba1 
                 BAB78601 
                 Cry32Ca1 
                 BAB78602 
               
               
                   
                 Cry32Cb1 
                 KC156708 
                 Cry32Da1 
                 BAB78603 
               
               
                   
                 Cry32Ea1 
                 GU324274 
                 Cry32Ea2 
                 KC156686 
               
               
                   
                 Cry32Eb1 
                 KC156663 
                 Cry32Fa1 
                 KC156656 
               
               
                   
                 Cry32Ga1 
                 KC156657 
                 Cry32Ha1 
                 KC156661 
               
               
                   
                 Cry32Hb1 
                 KC156666 
                 Cry32Ia1 
                 KC156667 
               
               
                   
                 Cry32Ja1 
                 KC156685 
                 Cry32Ka1 
                 KC156688 
               
               
                   
                 Cry32La1 
                 KC156689 
                 Cry32Ma1 
                 KC156690 
               
               
                   
                 Cry32Mb1 
                 KC156704 
                 Cry32Na1 
                 KC156691 
               
               
                   
                 Cry32Oa1 
                 KC156703 
                 Cry32Pa1 
                 KC156705 
               
               
                   
                 Cry32Qa1 
                 KC156706 
                 Cry32Ra1 
                 KC156707 
               
               
                   
                 Cry32Sa1 
                 KC156709 
                 Cry32Ta1 
                 KC156710 
               
               
                   
                 Cry32Ua1 
                 KC156655 
                 Cry33Aa1 
                 AAL26871 
               
               
                   
                 Cry34Aa1 
                 AAG50341 
                 Cry34Aa2 
                 AAK64560 
               
               
                   
                 Cry34Aa3 
                 AAT29032 
                 Cry34Aa4 
                 AAT29030 
               
               
                   
                 Cry34Ab1 
                 AAG41671 
                 Cry34Ac1 
                 AAG50118 
               
               
                   
                 Cry34Ac2 
                 AAK64562 
                 Cry34Ac3 
                 AAT29029 
               
               
                   
                 Cry34Ba1 
                 AAK64565 
                 Cry34Ba2 
                 AAT29033 
               
               
                   
                 Cry34Ba3 
                 AAT29031 
                 Cry35Aa1 
                 AAG50342 
               
               
                   
                 Cry35Aa2 
                 AAK64561 
                 Cry35Aa3 
                 AAT29028 
               
               
                   
                 Cry35Aa4 
                 AAT29025 
                 Cry35Ab1 
                 AAG41672 
               
               
                   
                 Cry35Ab2 
                 AAK64563 
                 Cry35Ab3 
                 AY536891 
               
               
                   
                 Cry35Ac1 
                 AAG50117 
                 Cry35Ba1 
                 AAK64566 
               
               
                   
                 Cry35Ba2 
                 AAT29027 
                 Cry35Ba3 
                 AAT29026 
               
               
                   
                 Cry36Aa1 
                 AAK64558 
                 Cry37Aa1 
                 AAF76376 
               
               
                   
                 Cry38Aa1 
                 AAK64559 
                 Cry39Aa1 
                 BAB72016 
               
               
                   
                 Cry40Aa1 
                 BAB72018 
                 Cry40Ba1 
                 BAC77648 
               
               
                   
                 Cry40Ca1 
                 EU381045 
                 Cry40Da1 
                 ACF15199 
               
               
                   
                 Cry41Aa1 
                 BAD35157 
                 Cry41Ab1 
                 BAD35163 
               
               
                   
                 Cry41Ba1 
                 HM461871 
                 Cry41Ba2 
                 ZP_04099652 
               
               
                   
                 Cry42Aa1 
                 BAD35166 
                 Cry43Aa1 
                 BAD15301 
               
               
                   
                 Cry43Aa2 
                 BAD95474 
                 Cry43Ba1 
                 BAD15303 
               
               
                   
                 Cry43Ca1 
                 KC156676 
                 Cry43Cb1 
                 KC156695 
               
               
                   
                 Cry43Cc1 
                 KC156696 
                 Cry43-like 
                 BAD15305 
               
               
                   
                 Cry44Aa 
                 Cry44Aa 
                 Cry45Aa 
                 BAD22577 
               
               
                   
                 Cry46Aa 
                 BAC79010 
                 Cry46Aa2 
                 BAG68906 
               
               
                   
                 Cry46Ab 
                 BAD35170 
                 Cry47Aa 
                 AAY24695 
               
               
                   
                 Cry48Aa 
                 CAJ18351 
                 Cry48Aa2 
                 CAJ86545 
               
               
                   
                 Cry48Aa3 
                 CAJ86546 
                 Cry48Ab 
                 CAJ86548 
               
               
                   
                 Cry48Ab2 
                 CAJ86549 
                 Cry49Aa 
                 CAH56541 
               
               
                   
                 Cry49Aa2 
                 CAJ86541 
                 Cry49Aa3 
                 CAJ86543 
               
               
                   
                 Cry49Aa4 
                 CAJ86544 
                 Cry49Ab1 
                 CAJ86542 
               
               
                   
                 Cry50Aa1 
                 BAE86999 
                 Cry50Ba1 
                 GU446675 
               
               
                   
                 Cry50Ba2 
                 GU446676 
                 Cry51Aa1 
                 ABI14444 
               
               
                   
                 Cry51Aa2 
                 GU570697 
                 Cry52Aa1 
                 EF613489 
               
               
                   
                 Cry52Ba1 
                 FJ361760 
                 Cry53Aa1 
                 EF633476 
               
               
                   
                 Cry53Ab1 
                 FJ361759 
                 Cry54Aa1 
                 ACA52194 
               
               
                   
                 Cry54Aa2 
                 GQ140349 
                 Cry54Ba1 
                 GU446677 
               
               
                   
                 Cry55Aa1 
                 ABW88932 
                 Cry54Ab1 
                 JQ916908 
               
               
                   
                 Cry55Aa2 
                 AAE33526 
                 Cry56Aa1 
                 ACU57499 
               
               
                   
                 Cry56Aa2 
                 GQ483512 
                 Cry56Aa3 
                 JX025567 
               
               
                   
                 Cry57Aa1 
                 ANC87261 
                 Cry58Aa1 
                 ANC87260 
               
               
                   
                 Cry59Ba1 
                 JN790647 
                 Cry59Aa1 
                 ACR43758 
               
               
                   
                 Cry60Aa1 
                 ACU24782 
                 Cry60Aa2 
                 EAO57254 
               
               
                   
                 Cry60Aa3 
                 EEM99278 
                 Cry60Ba1 
                 GU810818 
               
               
                   
                 Cry60Ba2 
                 EAO57253 
                 Cry60Ba3 
                 EEM99279 
               
               
                   
                 Cry61Aa1 
                 HM035087 
                 Cry61Aa2 
                 HM132125 
               
               
                   
                 Cry61Aa3 
                 EEM19308 
                 Cry62Aa1 
                 HM054509 
               
               
                   
                 Cry63Aa1 
                 BAI44028 
                 Cry64Aa1 
                 BAJ05397 
               
               
                   
                 Cry65Aa1 
                 HM461868 
                 Cry65Aa2 
                 ZP_04123838 
               
               
                   
                 Cry66Aa1 
                 HM485581 
                 Cry66Aa2 
                 ZP_04099945 
               
               
                   
                 Cry67Aa1 
                 HM485582 
                 Cry67Aa2 
                 ZP_04148882 
               
               
                   
                 Cry68Aa1 
                 HQ113114 
                 Cry69Aa1 
                 HQ401006 
               
               
                   
                 Cry69Aa2 
                 JQ821388 
                 Cry69Ab1 
                 JN209957 
               
               
                   
                 Cry70Aa1 
                 JN646781 
                 Cry70Ba1 
                 ADO51070 
               
               
                   
                 Cry70Bb1 
                 EEL67276 
                 Cry71Aa1 
                 JX025568 
               
               
                   
                 Cry72Aa1 
                 JX025569 
                 Cyt1Aa 
                 X03182 
               
               
                   
                 Cyt1Ab 
                 X98793 
                 Cyt1B 
                 U37196 
               
               
                   
                 Cyt2A 
                 Z14147 
                 Cyt2B 
                 U52043 
               
               
                   
                   
               
               
                   
                 * The amino acid and corresponding nucleotide sequences of the accession numbers in Table 2 are herein incorporated by reference. 
               
            
           
         
       
     
     Examples of δ-endotoxins also include but are not limited to Cry1A proteins of U.S. Pat. Nos. 5,880,275 and 7,858,849; a DIG-3 or DIG-11 toxin (N-terminal deletion of α-helix 1 and/or α-helix 2 variants of cry proteins such as Cry1A, Cry3A) of U.S. Pat. Nos. 8,304,604, 8,304,605 and 8,476,226; Cry1B of U.S. patent application Ser. No. 10/525,318; Cry1C of U.S. Pat. No. 6,033,874; Cry1F of U.S. Pat. Nos. 5,188,960 and 6,218,188; Cry1A/F chimeras of U.S. Pat. Nos. 7,070,982; 6,962,705 and 6,713,063); a Cry2 protein such as Cry2Ab protein of U.S. Pat. No. 7,064,249); a Cry3A protein including but not limited to an engineered hybrid insecticidal protein (eHIP) created by fusing unique combinations of variable regions and conserved blocks of at least two different Cry proteins (US Patent Application Publication Number 2010/0017914); a Cry4 protein; a Cry5 protein; a Cry6 protein; Cry8 proteins of U.S. Pat. Nos. 7,329,736, 7,449,552, 7,803,943, 7,476,781, 7,105,332, 7,378,499 and 7,462,760; a Cry9 protein such as such as members of the Cry9A, Cry9B, Cry9C, Cry9D, Cry9E and Cry9F families; a Cry15 protein of Naimov, et al., (2008)  Applied and Environmental Microbiology,  74:7145-7151; a Cry22, a Cry34Ab1 protein of U.S. Pat. Nos. 6,127,180, 6,624,145 and 6,340,593; a CryET33 and cryET34 protein of U.S. Pat. Nos. 6,248,535, 6,326,351, 6,399,330, 6,949,626, 7,385,107 and 7,504,229; a CryET33 and CryET34 homologs of US Patent Publication Number 2006/0191034, 2012/0278954, and PCT Publication Number WO 2012/139004; a Cry35Ab1 protein of U.S. Pat. Nos. 6,083,499, 6,548,291 and 6,340,593; a Cry46 protein, a Cry 51 protein, a Cry binary toxin; a TIC901 or related toxin; TIC807 of US Patent Application Publication Number 2008/0295207; ET29, ET37, TIC809, TIC810, TIC812, TIC127, TIC128 of PCT US 2006/033867; TIC853 toxins of U.S. Pat. No. 8,513,494, AXMI-027, AXMI-036, and AXMI-038 of U.S. Pat. No. 8,236,757; AXMI-031, AXMI-039, AXMI-040, AXMI-049 of U.S. Pat. No. 7,923,602; AXMI-018, AXMI-020 and AXMI-021 of WO 2006/083891; AXMI-010 of WO 2005/038032; AXMI-003 of WO 2005/021585; AXMI-008 of US Patent Application Publication Number 2004/0250311; AXMI-006 of US Patent Application Publication Number 2004/0216186; AXMI-007 of US Patent Application Publication Number 2004/0210965; AXMI-009 of US Patent Application Number 2004/0210964; AXMI-014 of US Patent Application Publication Number 2004/0197917; AXMI-004 of US Patent Application Publication Number 2004/0197916; AXMI-028 and AXMI-029 of WO 2006/119457; AXMI-007, AXMI-008, AXMI-0080rf2, AXMI-009, AXMI-014 and AXMI-004 of WO 2004/074462; AXMI-150 of U.S. Pat. No. 8,084,416; AXMI-205 of US Patent Application Publication Number 2011/0023184; AXMI-011, AXMI-012, AXMI-013, AXMI-015, AXMI-019, AXMI-044, AXMI-037, AXMI-043, AXMI-033, AXMI-034, AXMI-022, AXMI-023, AXMI-041, AXMI-063 and AXMI-064 of US Patent Application Publication Number 2011/0263488; AXMI-R1 and related proteins of US Patent Application Publication Number 2010/0197592; AXMI221Z, AXMI222z, AXMI223z, AXMI224z and AXMI225z of WO 2011/103248; AXMI218, AXMI219, AXMI220, AXMI226, AXMI227, AXMI228, AXMI229, AXMI230 and AXMI231 of WO 2011/103247; AXMI-115, AXMI-113, AXMI-005, AXMI-163 and AXMI-184 of U.S. Pat. No. 8,334,431; AXMI-001, AXMI-002, AXMI-030, AXMI-035 and AXMI-045 of US Patent Application Publication Number 2010/0298211; AXMI-066 and AXMI-076 of US Patent Application Publication Number 2009/0144852; AXMI128, AXMI130, AXMI131, AXMI133, AXMI140, AXMI141, AXMI142, AXMI143, AXMI144, AXMI146, AXMI148, AXMI149, AXMI152, AXMI153, AXMI154, AXMI155, AXMI156, AXMI157, AXMI158, AXMI162, AXMI165, AXMI166, AXMI167, AXMI168, AXMI169, AXMI170, AXMI171, AXMI172, AXMI173, AXMI174, AXMI175, AXMI176, AXMI177, AXMI178, AXMI179, AXMI180, AXMI181, AXMI182, AXMI185, AXMI186, AXMI187, AXM1188, AXM1189 of U.S. Pat. No. 8,318,900; AXM1079, AXM1080, AXM1081, AXMI082, AXMI091, AXMI092, AXMI096, AXMI097, AXMI098, AXMI099, AXMI100, AXMI101, AXM1102, AXM1103, AXM1104, AXM1107, AXM1108, AXM1109, AXMI110, AXMI111, AXMI112, AXMI114, AXMI116, AXMI117, AXMI118, AXMI119, AXMI120, AXMI121, AXMI122, AXMI123, AXMI124, AXMI1257, AXMI1268, AXMI127, AXMI129, AXMI164, AXMI151, AXMI161, AXMI183, AXMI132, AXMI138, AXMI137 of US Patent Application Publication Number 2010/0005543, cry proteins such as Cry1A and Cry3A having modified proteolytic sites of U.S. Pat. No. 8,319,019; a Cry1Ac, Cry2Aa and Cry1Ca toxin protein from  Bacillus thuringiensis  strain VBTS 2528 of US Patent Application Publication Number 2011/0064710. Other Cry proteins are well known to one skilled in the art (see, Crickmore, et al., “ Bacillus thuringiensis  toxin nomenclature” (2011), at lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/which can be accessed on the world-wide web using the “www” prefix). The insecticidal activity of Cry proteins is well known to one skilled in the art (for review, see, van Frannkenhuyzen, (2009)  J. Invert. Path.  101:1-16). The use of Cry proteins as transgenic plant traits is well known to one skilled in the art and Cry-transgenic plants including but not limited to plants expressing Cry1Ac, Cry1Ac+Cry2Ab, Cry1Ab, Cry1A.105, Cry1F, Cry1Fa2, Cry1F+Cry1Ac, Cry2Ab, Cry3A, mCry3A, Cry3Bb1, Cry34Ab1, Cry35Ab1, Vip3A, mCry3A, Cry9c and CBI-Bt have received regulatory approval (see, Sanahuja, (2011)  Plant Biotech Journal  9:283-300 and the CERA (2010) GM Crop Database Center for Environmental Risk Assessment (CERA), ILSI Research Foundation, Washington D.C. at cera-gmc.org/index.php?action=gm_crop_database, which can be accessed on the world-wide web using the “www” prefix). 
     The primary structure of a pesticidal polypeptide can be used in its native form or modified in order to introduce amino acid residues (either through addition or substitution) that contribute to or are suspected of contributing to the pesticidal activity of at least one pesticidal polypeptide. Functional data and results from structure-function analyses can be consulted to identify sequences and/or amino acid residues that contribute to pesticidal activity that should be retained or introduced into the candidate polypeptide sequence. Those residues that contribute to or are suspected of contributing to pesticidal activity include those residues that enhance efficacy or those that dictate pesticidal specificity, including those residues that narrow or broaden the range of pests of pesticidal proteins. 
     For example, the aromaticity of the tyrosine and phenylalanine residues at position 249 and 264, respectively, in helix 7 of the Bt Cry4Ba toxin has been found to be important for toxicity (Tiewsiri and Angsuthanasombat (2007)  J Biochem Mol Biol  40:163-171). Thus, in those embodiments wherein the candidate polypeptide is homologous with the Bt Cry4Ba toxin, the residues at the positions corresponding to positions 249 and 264 of Cry4Ba should be maintained if they are aromatic or modified if they are non-aromatic. 
     Further, additional mutations can be introduced into pesticidal polypeptide sequence to improve the pesticidal activity. For example, as described in U.S. Pat. Nos. 7,462,760 and 7,105,332 (each of which are herein incorporated by reference in its entirety), mutations can be introduced into the candidate polypeptide sequence to destroy proteolytic sites to protect the polypeptide from degradative digestion, for example, by plant proteases. As a further example, the toxicity of Bt Cry proteins can be improved by introducing at least one more protease-sensitive site (e.g., trypsin cleavage site) into the region located between alpha helices 3 and 4 of domain 1 of the endotoxin protein (see U.S. Patent Application Publication No. US2004/0091505, which is herein incorporated by reference in its entirety). As another non-limiting example, a protease-sensitive site that is readily cleaved by insect chymotrypsin, e.g., a chymotrypsin found in the bertha armyworm or the corn earworm (Hegedus et al. (2003)  Arch. Insect Biochem. Physiol.  53: 30-47; and Lenz et al. (1991)  Arch. Insect Biochem. Physiol.  16: 201-212), may be added to the candidate polypeptide sequence to provide improved toxicity to the polypeptide. 
     In certain embodiments of the invention, the Cry protein comprises the amino acid sequence set forth in SEQ ID NO: 8, 10, 12 or 14 or fragment or variant thereof. Any nucleotide sequence encoding the amino acid sequence set forth in 8, 10, 12 or 14 or fragment or variant thereof, can be used in the methods and compositions of the present invention including, but not limited to the nucleotides sequences encoding SEQ ID NOS: 8, 10, 12, and 14 which are set forth in SEQ ID NOS: 7, 9, 11, and 13, respectively. In some embodiments of the invention, the nucleotide sequences of the invention will be optimized for expression in a host organism or cell of interest, particularly a plant, more particularly a crop plant, most particularly a maize plant. 
     Methods of measuring pesticidal activity by insect bioassays are well known in the art. See, e.g., Brooke et al. (2001)  Bull. Entomol. Res.  91:265-272; Chen et al. (2007)  Proc. Natl. Acad. Sci. USA  104:13901-13906; Crespo et al. (2008)  Appl. Environ. Microb.  74:130-135; Kham bay et al. (2003)  Pest Manag. Sci.  59:174-182; Liu &amp; Dean (2006)  Protein Eng. Des. Sel.  19:107-111; Marrone et al. (1985)  J. Econ. Entomol.  78:290-293; Robertson et al.,  Pesticide Bioassays with Arthropods  (2 nd  ed., CRC Press 2007); Scott &amp; McKibben (1976)  J. Econ. Entomol.  71:343-344; Strickman (1985)  Bull. Environ. Contam. Toxicol.  35:133-142; and Verma et al. (1982)  Water Res.  16 525-529; as well as U.S. Pat. No. 6,268,181. Examples of insect bioassays include, but are not limited to, pest mortality, pest weight loss, pest repellency, pest attraction, and other behavioral and physical changes of the pest after feeding and exposure to a pesticide or pesticidal polypeptide for an appropriate length of time. General methods include addition of the pesticide, pesticidal polypeptide or an organism having the pesticidal polypeptide to the diet source in an enclosed container. See, e.g., U.S. Pat. Nos. 6,339,144 and 6,570,005. 
     Further, a nucleic acid encoding the novel chimeric pesticidal polypeptide can be derived from the amino acid sequence and can be generated using any method known in the art. Therefore, novel isolated pesticidal polypeptides and isolated nucleic acid molecules encoding the same are provided. An “isolated” or “purified” polynucleotide or protein or biologically active fragment thereof, is substantially or essentially free from components that normally accompany or interact with the polynucleotide or protein as found in its naturally occurring environment. Thus, an isolated or purified polynucleotide or protein is substantially free of other cellular material or culture medium when produced by recombinant techniques or substantially free of chemical precursors or other chemicals when chemically synthesized. Optimally, an “isolated” polynucleotide is free of sequences (optimally protein encoding sequences) that naturally flank the polynucleotide (i.e., sequences located at the 5′ and 3′ ends of the polynucleotide) in the genomic DNA of the organism from which the polynucleotide is derived. For example, in various embodiments, the isolated polynucleotide can contain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb of nucleotide sequence that naturally flank the polynucleotide in genomic DNA of the cell from which the polynucleotide is derived. A protein that is substantially free of cellular material includes preparations of protein having less than about 30%, 20%, 10%, 5% or 1% (by dry weight) of contaminating protein. When the protein of the invention or biologically active fragment thereof is recombinantly produced, optimally culture medium represents less than about 30%, 20%, 10%, 5% or 1% (by dry weight) of chemical precursors or non-protein-of-interest chemicals. 
     Further provided are novel chimeric pesticidal polypeptides comprising fragments and variants of a solubility-enhancing polypeptide and/or pesticidal protein as well as nucleic acid molecules encoding such novel chimeric pesticidal polypeptides. Fragments and variants of the novel chimeric pesticidal polypeptides and chimeric pesticidal polypeptide-encoding nucleic acid molecules are also provided. By “fragment” is intended a portion of the polynucleotide or a portion of the amino acid sequence and hence protein encoded thereby. Fragments of a polynucleotide may encode protein fragments that retain solubility-enhancing activity and/or pesticidal activity. Alternatively, fragments of a polynucleotide that are useful as hybridization probes generally do not encode fragment proteins retaining a solubility-enhancing and/or pesticidal activity. Thus, fragments of a nucleotide sequence may range from at least about 20 nucleotides, about 50 nucleotides, about 100 nucleotides, and up to the full-length polynucleotide encoding the novel chimeric pesticidal polypeptides. 
     A fragment of a pesticide-encoding polynucleotide that encodes a biologically active fragment of a solubility-enhancing polypeptide or pesticidal protein will encode at least 15, 25, 30, 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1000 or 1,100 contiguous amino acids or up to the total number of amino acids present in a chimeric pesticidal polypeptide, a solubility-enhancing polypeptide or a pesticidal protein of the invention. Fragments of a chimeric pesticidal polypeptide, solubility-enhancing polypeptide or a pesticidal protein that are useful as hybridization probes or PCR primers generally need not encode a biologically active fragment of a chimeric pesticidal polypeptide, a solubility-enhancing polypeptide or a pesticidal protein. 
     Thus, a fragment of a chimeric pesticidal polypeptide, a solubility-enhancing polypeptide or a pesticidal protein may encode a biologically active fragment of a pesticidal protein or it may be a fragment that can be used as a hybridization probe or PCR primer using methods well known in the art and disclosed elsewhere herein. A biologically active fragment of a pesticidal protein can be prepared by isolating a portion of one of the pesticide-encoding polynucleotides, expressing the encoded portion of the pesticidal protein (e.g., by recombinant expression in vitro), and assessing the activity of the encoded portion of the pesticidal protein. Polynucleotides that are fragments of a chimeric pesticidal polypeptide, a solubility-enhancing polypeptide or a pesticidal protein sequence comprise at least 16, 20, 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900, 2000, 2500 or 3000 contiguous nucleotides or up to the number of nucleotides present in a full-length pesticide-encoding polynucleotide discovered using the methods disclosed herein. 
     “Variants” is intended to mean substantially similar sequences. For polynucleotides, a variant comprises a polynucleotide having deletions (i.e., truncations) at the 5′ and/or 3′ end; deletion and/or addition of one or more nucleotides at one or more internal sites in the native polynucleotide; and/or substitution of one or more nucleotides at one or more sites in the native polynucleotide. As used herein, a “native” polynucleotide or polypeptide comprises a naturally occurring nucleotide sequence or amino acid sequence, respectively. For polynucleotides, conservative variants include those sequences that, because of the degeneracy of the genetic code, encode the amino acid sequence of one of the chimeric pesticidal polypeptide, solubility-enhancing polypeptides or pesticidal proteins of the invention. Naturally occurring allelic variants such as these can be identified with the use of well-known molecular biology techniques, as, for example, with polymerase chain reaction (PCR) and hybridization techniques as outlined below. Variant polynucleotides also include synthetically derived polynucleotides, such as those generated, for example, by using site-directed mutagenesis but which still encode a chimeric pesticidal polypeptide, a solubility-enhancing polypeptide or a pesticidal protein of the invention. Generally, variants of a particular polynucleotide of the invention will have at least about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to that particular polynucleotide as determined by sequence alignment programs and parameters as described elsewhere herein. 
     Variants of a particular polynucleotide of the invention (i.e., the reference polynucleotide) can also be evaluated by comparison of the percent sequence identity between the polypeptide encoded by a variant polynucleotide and the polypeptide encoded by the reference polynucleotide. Thus, for example, an isolated polynucleotide that encodes a polypeptide with a given percent sequence identity to the polypeptide of SEQ ID NO: 2 is disclosed. Percent sequence identity between any two polypeptides can be calculated using sequence alignment programs and parameters described elsewhere herein. Where any given pair of polynucleotides of the invention is evaluated by comparison of the percent sequence identity shared by the two polypeptides they encode, the percent sequence identity between the two encoded polypeptides is at least about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity. 
     “Variant” protein is intended to mean a protein derived from the native protein by deletion (so-called truncation) of one or more amino acids at the N-terminal and/or C-terminal end of the native protein; deletion and/or addition of one or more amino acids at one or more internal sites in the native protein; or substitution of one or more amino acids at one or more sites in the native protein. Variant proteins encompassed by the present invention are biologically active, that is they continue to possess the desired biological activity of the native protein, that is, pesticidal activity as described herein. Such variants may result from, for example, genetic polymorphism or from human manipulation. Biologically active variants of a chimeric pesticidal polypeptide of the invention will have at least about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to the amino acid sequence for the native protein as determined by sequence alignment programs and parameters described elsewhere herein. A biologically active variant of a protein of the invention may differ from that protein by as few as 1-15 amino acid residues, as few as 1-10, such as 6-10, as few as 5, as few as 4, 3, 2 or even 1 amino acid residue. 
     The novel chimeric pesticidal polypeptide made using the presently disclosed methods may be altered in various ways including amino acid substitutions, deletions, truncations, and insertions to the solubility-enhancing polypeptide and/or the pesticidal polypeptide portion of the chimeric pesticidal polypeptide. Methods for such manipulations are generally known in the art. For example, amino acid sequence variants and fragments of the chimeric pesticidal polypeptides, pesticidal polypeptides, and solubility-enhancing polypeptide s can be prepared by mutations in the DNA. Methods for mutagenesis and polynucleotide alterations are well known in the art. See, for example, Kunkel (1985)  Proc. Natl. Acad. Sci. USA  82:488-492; Kunkel et al. (1987)  Methods in Enzymol.  154:367-382; U.S. Pat. No. 4,873,192; Walker and Gaastra, eds. (1983)  Techniques in Molecular Biology  (MacMillan Publishing Company, New York) and the references cited therein. Guidance as to appropriate amino acid substitutions that do not affect biological activity of the protein of interest may be found in the model of Dayhoff et al. (1978)  Atlas of Protein Sequence and Structure  (Natl. Biomed. Res. Found., Washington, D.C.), herein incorporated by reference. Conservative substitutions, such as exchanging one amino acid with another having similar properties, may be optimal. 
     The deletions, insertions, and substitutions of the novel chimeric pesticidal polypeptide sequences, pesticidal polypeptide sequences and solubility-enhancing polypeptide sequences encompassed herein are not expected to produce radical changes in the characteristics of the protein. However, when it is difficult to predict the exact effect of the substitution, deletion or insertion in advance of doing so, one skilled in the art will appreciate that the effect will be evaluated by routine screening assays. That is, the pesticidal activity can be evaluated using an insect feeding bioassays as described elsewhere herein. Preferably, the biologically activity of a solubility-enhancing polypeptide or fragment or variant thereof is evaluated by operably linking the solubility-enhancing polypeptide, fragment or variant to a pesticidal polypeptide and assaying the pesticidal activity of the resulting chimeric pesticidal polypeptide using the methods disclosed herein or otherwise known in the art. The pesticidal activity of the chimeric pesticidal polypeptide can be compared to the pesticidal activity of the pesticidal peptide (i.e., without the solubility-enhancing polypeptide, fragment or variant) to determine if the solubility-enhancing polypeptide, fragment or variant increases the insecticidal activity the chimeric pesticidal polypeptide. Preferably, in any insect feeding assays in which the chimeric pesticidal polypeptide its corresponding pesticidal polypeptide, the amount of the respective polypeptides that are fed to the insects will be adjusted to take into the account the large molecular weight of the chimeric pesticidal polypeptide such that pesticidal activity comparisons will effectively be made on a per molecule or per mole basis. Furthermore, it is recognized that insecticidal activity of chimeric pesticidal polypeptides with different solubility-enhancing polypeptides or fragments or variants of a solubility-enhancing polypeptide can also evaluated and compared to one another in a like manner and preferably with adjustments for any differences in molecular weights between the chimeric pesticidal polypeptides that are being compared. 
     Variant polynucleotides and proteins also encompass sequences and proteins derived from a mutagenic and recombinogenic procedure such as DNA shuffling. With such a procedure, one or more different pesticide-coding sequences can be manipulated to create a new pesticidal polypeptide possessing the desired properties and these new pesticidal proteins can be used in the methods disclosed herein to make chimeric pesticidal polypepties. In this manner, libraries of recombinant polynucleotides are generated from a population of related sequence polynucleotides comprising sequence regions that have substantial sequence identity and can be homologously recombined in vitro or in vivo. Strategies for such DNA shuffling are known in the art. See, for example, Stemmer (1994)  Proc. Natl. Acad. Sci. USA  91:10747-10751; Stemmer (1994)  Nature  370:389-391; Crameri et al. (1997)  Nature Biotech.  15:436-438; Moore et al. (1997)  J. Mol. Biol.  272:336-347; Zhang et al. (1997)  Proc. Natl. Acad. Sci. USA  94:4504-4509; Crameri et al. (1998)  Nature  391:288-291; and U.S. Pat. Nos. 5,605,793 and 5,837,458. 
     The following terms are used to describe the sequence relationships between two or more polynucleotides or polypeptides: (a) “reference sequence”, (b) “comparison window”, (c) “sequence identity”, and, (d) “percentage of sequence identity.” 
     (a) As used herein, “reference sequence” is a defined sequence used as a basis for sequence comparison. A reference sequence may be a subset or the entirety of a specified sequence; for example, as a segment of a full-length cDNA or gene sequence or the complete cDNA or gene sequence. 
     (b) As used herein, “comparison window” makes reference to a contiguous and specified segment of a polynucleotide sequence, wherein the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two polynucleotides. Generally, the comparison window is at least 20 contiguous nucleotides in length, and optionally can be 30, 40, 50, 100 or longer. Those of skill in the art understand that to avoid a high similarity to a reference sequence due to inclusion of gaps in the polynucleotide sequence a gap penalty is typically introduced and is subtracted from the number of matches. 
     Methods of alignment of sequences for comparison are well known in the art. Thus, the determination of percent sequence identity between any two sequences can be accomplished using a mathematical algorithm. Non-limiting examples of such mathematical algorithms are the algorithm of Myers and Miller (1988)  CABIOS  4:11-17; the local alignment algorithm of Smith et al. (1981)  Adv. Appl. Math.  2:482; the global alignment algorithm of Needleman and Wunsch (1970)  J. Mol. Biol.  48:443-453; the search-for-local alignment method of Pearson and Lipman (1988)  Proc. Natl. Acad. Sci.  85:2444-2448; the algorithm of Karlin and Altschul (1990)  Proc. Natl. Acad. Sci. USA  872264, modified as in Karlin and Altschul (1993)  Proc. Natl. Acad. Sci. USA  90:5873-5877. 
     Computer implementations of these mathematical algorithms can be utilized for comparison of sequences to determine sequence identity. Such implementations include, but are not limited to: CLUSTAL in the PC/Gene program (available from Intelligenetics, Mountain View, Calif.); the ALIGN program (Version 2.0) and GAP, BESTFIT, BLAST, FASTA, and TFASTA in the GCG Wisconsin Genetics Software Package, Version 10 (available from Accelrys Inc., 9685 Scranton Road, San Diego, Calif., USA). Alignments using these programs can be performed using the default parameters. The CLUSTAL program is well described by Higgins et al. (1988)  Gene  73:237-244 (1988); Higgins et al. (1989)  CABIOS  5:151-153; Corpet et al. (1988)  Nucleic Acids Res.  16:10881-90; Huang et al. (1992)  CABIOS  8:155-65; and Pearson et al. (1994)  Meth. Mol. Biol.  24:307-331. The ALIGN program is based on the algorithm of Myers and Miller (1988) supra. A PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used with the ALIGN program when comparing amino acid sequences. The BLAST programs of Altschul et al (1990)  J. Mol. Biol.  215:403 are based on the algorithm of Karlin and Altschul (1990) supra. BLAST nucleotide searches can be performed with the BLASTN program, score=100, wordlength=12, to obtain nucleotide sequences homologous to a nucleotide sequence encoding a protein of the invention. BLAST protein searches can be performed with the BLASTX program, score=50, wordlength=3, to obtain amino acid sequences homologous to a protein or polypeptide of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST (in BLAST 2.0) can be utilized as described in Altschul et al. (1997)  Nucleic Acids Res.  25:3389. Alternatively, PSI-BLAST (in BLAST 2.0) can be used to perform an iterated search that detects distant relationships between molecules. See Altschul et al. (1997) supra. When utilizing BLAST, Gapped BLAST, PSI-BLAST, the default parameters of the respective programs (e.g., BLASTN for nucleotide sequences, BLASTX for proteins) can be used. See www.ncbi.nlm.nih.gov. Alignment may also be performed manually by inspection. 
     Unless otherwise stated, sequence identity/similarity values provided herein refer to the value obtained using GAP Version 10 using the following parameters: % identity and % similarity for a nucleotide sequence using GAP Weight of 50 and Length Weight of 3, and the nwsgapdna.cmp scoring matrix; % identity and % similarity for an amino acid sequence using GAP Weight of 8 and Length Weight of 2, and the BLOSUM62 scoring matrix; or any equivalent program thereof. By “equivalent program” is intended any sequence comparison program that, for any two sequences in question, generates an alignment having identical nucleotide or amino acid residue matches and an identical percent sequence identity when compared to the corresponding alignment generated by GAP Version 10. 
     GAP uses the algorithm of Needleman and Wunsch (1970)  J. Mol. Biol.  48:443-453, to find the alignment of two complete sequences that maximizes the number of matches and minimizes the number of gaps. GAP considers all possible alignments and gap positions and creates the alignment with the largest number of matched bases and the fewest gaps. It allows for the provision of a gap creation penalty and a gap extension penalty in units of matched bases. GAP must make a profit of gap creation penalty number of matches for each gap it inserts. If a gap extension penalty greater than zero is chosen, GAP must, in addition, make a profit for each gap inserted of the length of the gap times the gap extension penalty. Default gap creation penalty values and gap extension penalty values in Version 10 of the GCG Wisconsin Genetics Software Package for protein sequences are 8 and 2, respectively. For nucleotide sequences the default gap creation penalty is 50 while the default gap extension penalty is 3. The gap creation and gap extension penalties can be expressed as an integer selected from the group of integers consisting of from 0 to 200. Thus, for example, the gap creation and gap extension penalties can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65 or greater. 
     GAP presents one member of the family of best alignments. There may be many members of this family, but no other member has a better quality. GAP displays four figures of merit for alignments: Quality, Ratio, Identity, and Similarity. The Quality is the metric maximized in order to align the sequences. Ratio is the quality divided by the number of bases in the shorter segment. Percent Identity is the percent of the symbols that actually match. Percent Similarity is the percent of the symbols that are similar. Symbols that are across from gaps are ignored. A similarity is scored when the scoring matrix value for a pair of symbols is greater than or equal to 0.50, the similarity threshold. The scoring matrix used in Version 10 of the GCG Wisconsin Genetics Software Package is BLOSUM62 (see Henikoff and Henikoff (1989)  Proc. Natl. Acad. Sci. USA  89:10915). 
     (c) As used herein, “sequence identity” or “identity” in the context of two polynucleotides or polypeptide sequences makes reference to the residues in the two sequences that are the same when aligned for maximum correspondence over a specified comparison window. When percentage of sequence identity is used in reference to proteins it is recognized that residue positions which are not identical often differ by conservative amino acid substitutions, where amino acid residues are substituted for other amino acid residues with similar chemical properties (e.g., charge or hydrophobicity) and therefore do not change the functional properties of the molecule. When sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Sequences that differ by such conservative substitutions are said to have “sequence similarity” or “similarity”. Means for making this adjustment are well known to those of skill in the art. Typically this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated, e.g., as implemented in the program PC/GENE (Intelligenetics, Mountain View, Calif.). 
     (d) As used herein, “percentage of sequence identity” means the value determined by comparing two optimally aligned sequences over a comparison window, wherein the portion of the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison, and multiplying the result by 100 to yield the percentage of sequence identity. 
     In hybridization techniques, all or part of a known polynucleotide is used as a probe that selectively hybridizes to other corresponding polynucleotides present in a population of cloned genomic DNA fragments or cDNA fragments (i.e., genomic or cDNA libraries) from a chosen organism. The hybridization probes may be genomic DNA fragments, cDNA fragments, RNA fragments or other oligonucleotides, and may be labeled with a detectable group such as  32 P or any other detectable marker. Thus, for example, probes for hybridization can be made by labeling synthetic oligonucleotides based on the babyboom polynucleotide. Methods for preparation of probes for hybridization and for construction of cDNA and genomic libraries are generally known in the art and are disclosed in Sambrook et al. (1989)  Molecular Cloning: A Laboratory Manual  (2d ed., Cold Spring Harbor Laboratory Press, Plainview, N.Y.). 
     For example, the entire pesticide-encoding polynucleotide or one or more portions thereof, may be used as a probe capable of specifically hybridizing to corresponding pesticide-encoding polynucleotides and messenger RNAs. To achieve specific hybridization under a variety of conditions, such probes include sequences that are unique among pesticide-encoding polynucleotide sequences and are optimally at least about 10 nucleotides in length, and most optimally at least about 20 nucleotides in length. Such probes may be used to amplify corresponding pesticide-encoding polynucleotides from a chosen organism by PCR. This technique may be used to isolate additional coding sequences from a desired organism or as a diagnostic assay to determine the presence of coding sequences in an organism. Hybridization techniques include hybridization screening of plated DNA libraries (either plaques or colonies; see, for example, Sambrook et al. (1989)  Molecular Cloning: A Laboratory Manual  (2d ed., Cold Spring Harbor Laboratory Press, Plainview, N.Y.). 
     Hybridization of such sequences may be carried out under stringent conditions. By “stringent conditions” or “stringent hybridization conditions” is intended conditions under which a probe will hybridize to its target sequence to a detectably greater degree than to other sequences (e.g., at least 2-fold over background). Stringent conditions are sequence-dependent and will be different in different circumstances. By controlling the stringency of the hybridization and/or washing conditions, target sequences that are 100% complementary to the probe can be identified (homologous probing). Alternatively, stringency conditions can be adjusted to allow some mismatching in sequences so that lower degrees of similarity are detected (heterologous probing). Generally, a probe is less than about 1000 nucleotides in length, optimally less than 500 nucleotides in length. 
     Typically, stringent conditions will be those in which the salt concentration is less than about 1.5 M Na ion, typically about 0.01 to 1.0 M Na ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30° C. for short probes (e.g., 10 to 50 nucleotides) and at least about 60° C. for long probes (e.g., greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide. Exemplary low stringency conditions include hybridization with a buffer solution of 30 to 35% formamide, 1 M NaCl, 1% SDS (sodium dodecyl sulphate) at 37° C., and a wash in 1× to 2×SSC (20×SSC=3.0 M NaCl/0.3 M trisodium citrate) at 50 to 55° C. Exemplary moderate stringency conditions include hybridization in 40 to 45% formamide, 1.0 M NaCl, 1% SDS at 37° C., and a wash in 0.5× to 1× SSC at 55 to 60° C. Exemplary high stringency conditions include hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60 to 65° C. Optionally, wash buffers may comprise about 0.1% to about 1% SDS. Duration of hybridization is generally less than about 24 hours, usually about 4 to about 12 hours. The duration of the wash time will be at least a length of time sufficient to reach equilibrium. 
     Specificity is typically the function of post-hybridization washes, the critical factors being the ionic strength and temperature of the final wash solution. For DNA-DNA hybrids, the T m  can be approximated from the equation of Meinkoth and Wahl (1984)  Anal. Biochem.  138:267-284: T m =81.5° C.+16.6 (log M)+0.41 (% GC)−0.61 (% form)−500/L; where M is the molarity of monovalent cations, % GC is the percentage of guanosine and cytosine nucleotides in the DNA, % form is the percentage of formamide in the hybridization solution, and L is the length of the hybrid in base pairs. The T m  is the temperature (under defined ionic strength and pH) at which 50% of a complementary target sequence hybridizes to a perfectly matched probe. T m  is reduced by about 1° C. for each 1% of mismatching; thus, T m , hybridization, and/or wash conditions can be adjusted to hybridize to sequences of the desired identity. For example, if sequences with ≧90% identity are sought, the T m  can be decreased 10° C. Generally, stringent conditions are selected to be about 5° C. lower than the thermal melting point (T m ) for the specific sequence and its complement at a defined ionic strength and pH. However, severely stringent conditions can utilize a hybridization and/or wash at 1, 2, 3 or 4° C. lower than the thermal melting point (T m ); moderately stringent conditions can utilize a hybridization and/or wash at 6, 7, 8, 9 or 10° C. lower than the thermal melting point (T m ); low stringency conditions can utilize a hybridization and/or wash at 11, 12, 13, 14, 15 or 20° C. lower than the thermal melting point (T m ). Using the equation, hybridization and wash compositions, and desired T m , those of ordinary skill will understand that variations in the stringency of hybridization and/or wash solutions are inherently described. If the desired degree of mismatching results in a T m  of less than 45° C. (aqueous solution) or 32° C. (formamide solution), it is optimal to increase the SSC concentration so that a higher temperature can be used. An extensive guide to the hybridization of nucleic acids is found in Tijssen (1993)  Laboratory Techniques in Biochemistry and Molecular Biology—Hybridization with Nucleic Acid Probes,  Part I, Chapter 2 (Elsevier, New York); and Ausubel et al., eds. (1995)  Current Protocols in Molecular Biology,  Chapter 2 (Greene Publishing and Wiley-Interscience, New York). See Sambrook et al. (1989)  Molecular Cloning: A Laboratory Manual  (2d ed., Cold Spring Harbor Laboratory Press, Plainview, N.Y.). 
     The use of the term “polynucleotide” is not intended to limit the present invention to polynucleotides comprising DNA. Those of ordinary skill in the art will recognize that polynucleotides can comprise ribonucleotides and combinations of ribonucleotides and deoxyribonucleotides. Such deoxyribonucleotides and ribonucleotides include both naturally occurring molecules and synthetic analogues. The polynucleotides of the invention also encompass all forms of sequences including, but not limited to, single-stranded forms, double-stranded forms, hairpins, stem-and-loop structures, and the like. 
     Nucleic acids encoding the novel chimeric pesticidal polypeptides can be used in DNA constructs for expression in various hosts, including plants. Thus, expression cassettes comprising the novel chimeric pesticidal polypeptide-encoding nucleic acids for expression in the organism of interest are provided. The cassette will include 5′ and 3′ regulatory sequences operably linked to a novel chimeric pesticidal polypeptide-encoding polynucleotide. “Operably linked” is intended to mean a functional linkage between two or more elements. For example, an operable linkage between a polynucleotide of interest and a regulatory sequence (i.e., a promoter) is a functional link that allows for expression of the polynucleotide of interest. In the case of fusion proteins or fusion polypeptides, an operable linkage between, for example, a first amino amino sequence of interest and a second amino acid of interest results in a single amino acid sequence, which comprises both the first and second amino acid sequences, and wherein the C-terminal amino acid the first amino amino sequence is covalently attached to the N-terminal amino acid of the second amino acid sequence by a peptide bond. Operably linked elements may be contiguous or non-contiguous. The cassette may additionally contain at least one additional polynucleotide of interest to be cotransformed into the organism. Alternatively, the additional polynucleotide(s) of interest can be provided on multiple expression cassettes. Such an expression cassette is provided with a plurality of restriction sites and/or recombination sites for insertion of the pesticide-encoding polynucleotide to be under the transcriptional regulation of the regulatory regions. The expression cassette may additionally contain selectable marker genes. 
     The chimeric pesticidal polypeptides of the present invention comprise an amino acid sequence of a solubility-enhancing polypeptide operably linked to an amino acid sequence of a Cry endotoxin. It is recognized that in such an operable linkage, the amino acid sequence of the solubility-enhancing polypeptide can be contiguous to the amino acid sequence of the Cry endotoxin or can be separated by an intervening linker or other amino acid sequence. Likewise, the chimeric pesticidal polypeptide-encoding nucleic acid molecules of the present invention comprise a nucleotide sequence encoding a solubility-enhancing polypeptide operably linked to a nucleotide sequence encoding a Cry endotoxin. It is further recognized that in such an operable linkage, the nucleotide sequence encoding the solubility-enhancing polypeptide can be contiguous to the nucleotide sequence encoding the Cry endotoxin or can be separated by an intervening linker-encoding nucleotide sequence or other nucleotide sequence. 
     The expression cassette will include in the 5′-3′ direction of transcription, a transcriptional and translational initiation region (i.e., a promoter), a novel pesticide-encoding polynucleotide, and a transcriptional and translational termination region (i.e., termination region) functional in the organism to which the expression cassette is introduced. The regulatory regions (i.e., promoters, transcriptional regulatory regions, and translational termination regions) and/or the pesticide-encoding polynucleotide may be native/analogous to the host cell or to each other. Alternatively, the regulatory regions and/or the pesticide-encoding polynucleotide may be heterologous to the host cell or to each other. As used herein, “heterologous” in reference to a sequence is a sequence that originates from a foreign species or, if from the same species, is substantially modified from its native form in composition and/or genomic locus by deliberate human intervention. For example, a promoter operably linked to a heterologous polynucleotide is from a species different from the species from which the polynucleotide was derived or, if from the same/analogous species, one or both are substantially modified from their original form and/or genomic locus or the promoter is not the native promoter for the operably linked polynucleotide. 
     The termination region may be native with the transcriptional initiation region, may be native with the operably linked pesticidal polynucleotide of interest, may be native with the plant host or may be derived from another source (i.e., foreign or heterologous) to the promoter, the pesticidal polynucleotide of interest, the plant host or any combination thereof. Convenient termination regions are available from the Ti-plasmid of  A. tumefaciens,  such as the octopine synthase and nopaline synthase termination regions. See also Guerineau et al. (1991)  Mol. Gen. Genet.  262:141-144; Proudfoot (1991)  Cell  64:671-674; Sanfacon et al. (1991)  Genes Dev.  5:141-149; Mogen et al. (1990)  Plant Cell  2:1261-1272; Munroe et al. (1990)  Gene  91:151-158; Ballas et al. (1989)  Nucleic Acids Res.  17:7891-7903; and Joshi et al. (1987)  Nucleic Acids Res.  15:9627-9639. 
     Where appropriate, the polynucleotides may be optimized for increased expression in the transformed plant. That is, the polynucleotides can be synthesized using plant-preferred codons for improved expression. See, for example, Campbell and Gowri (1990)  Plant Physiol.  92:1-11 for a discussion of host-preferred codon usage. Methods are available in the art for synthesizing plant-preferred genes. See, for example, U.S. Pat. Nos. 5,380,831, and 5,436,391, and Murray et al. (1989)  Nucleic Acids Res.  17:477-498, herein incorporated by reference. 
     Additional sequence modifications are known to enhance gene expression in a cellular host. These include elimination of sequences encoding spurious polyadenylation signals, exon-intron splice site signals, transposon-like repeats, and other such well-characterized sequences that may be deleterious to gene expression. The G-C content of the sequence may be adjusted to levels average for a given cellular host, as calculated by reference to known genes expressed in the host cell. When possible, the sequence is modified to avoid predicted hairpin secondary mRNA structures. 
     The expression cassettes may additionally contain 5′ leader sequences. Such leader sequences can act to enhance translation. Translation leaders are known in the art and include: picornavirus leaders, for example, EMCV leader (Encephalomyocarditis 5′ noncoding region) (Elroy-Stein et al. (1989)  Proc. Natl. Acad. Sci. USA  86:6126-6130); potyvirus leaders, for example, TEV leader (Tobacco Etch Virus) (Gallie et al. (1995)  Gene  165(2):233-238), MDMV leader (Maize Dwarf Mosaic Virus) ( Virology  154:9-20), and human immunoglobulin heavy-chain binding protein (BiP) (Macejak et al. (1991)  Nature  353:90-94); untranslated leader from the coat protein mRNA of alfalfa mosaic virus (AMV RNA 4) (Jobling et al. (1987)  Nature  325:622-625); tobacco mosaic virus leader (TMV) (Gallie et al. (1989) in  Molecular Biology of RNA,  ed. Cech (Liss, N.Y.), pp. 237-256); and maize chlorotic mottle virus leader (MCMV) (Lommel et al. (1991)  Virology  81:382-385). See also, Della-Cioppa et al. (1987)  Plant Physiol.  84:965-968. 
     In preparing the expression cassette, the various DNA fragments may be manipulated, so as to provide for the DNA sequences in the proper orientation and, as appropriate, in the proper reading frame. Toward this end, adapters or linkers may be employed to join the DNA fragments or other manipulations may be involved to provide for convenient restriction sites, removal of superfluous DNA, removal of restriction sites or the like. For this purpose, in vitro mutagenesis, primer repair, restriction, annealing, resubstitutions, e.g., transitions and transversions, may be involved. 
     The chimeric pesticidal polypeptide-encoding nucleic acid molecules, expression cassettes comprising the same or the chimeric pesticidal polypeptides can be introduced into an organism or host cell, particularly a non-human host cell. Host cells may be prokaryotic cells such as  E. coli  or eukaryotic cells such as yeast, insect, amphibian or mammalian cells. In some examples, host cells are monocotyledonous or dicotyledonous plant cells. 
     The nucleic acid encoding the novel chimeric pesticidal polypeptides can be introduced into microorganisms that multiply on plants (epiphytes) to deliver chimeric pesticidal polypeptides to potential target pests. Alternatively, the chimeric pesticidal polypeptides can be directly introduced into such microorganisms. Epiphytes, for example, can be gram-positive or gram-negative bacteria. 
     Root-colonizing bacteria, for example, can be isolated from the plant of interest by methods known in the art. Specifically, a  Bacillus cereus  strain that colonizes roots can be isolated from roots of a plant (see, for example, Handelsman et al. (1991)  Appl. Environ. Microbiol.  56:713-718). Nucleic acids encoding the pesticidal proteins of the invention or the chimeric pesticidal polypeptides can be introduced into a root-colonizing  Bacillus cereus  by standard methods known in the art. 
     Nucleic acids encoding chimeric pesticidal polypeptides can be introduced, for example, into the root-colonizing  Bacillus  by means of electrotransformation. Specifically, nucleic acids encoding the pesticidal proteins can be cloned into a shuttle vector, for example, pHT3101 (Lerecius et al. (1989)  FEMS Microbiol. Letts.  60: 211-218. The shuttle vector pHT3101 containing the coding sequence for the particular chimeric pesticidal polypeptide-encoding nucleic acid can, for example, be transformed into the root-colonizing  Bacillus  by means of electroporation (Lerecius et al. (1989)  FEMS Microbiol. Letts.  60: 211-218). 
     Expression systems can be designed so that chimeric pesticidal polypeptides are secreted outside the cytoplasm of gram-negative bacteria, such as  E. coli,  for example. Advantages of having chimeric pesticidal polypeptides secreted are: (1) avoidance of potential cytotoxic effects of the chimeric pesticidal polypeptides expressed; and (2) improvement in the efficiency of purification of the chimeric pesticidal polypeptides, including, but not limited to, increased efficiency in the recovery and purification of the protein per volume cell broth and decreased time and/or costs of recovery and purification per unit protein. 
     Chimeric pesticidal polypeptides can be made to be secreted in  E. coli,  for example, by fusing an appropriate  E. coli  signal peptide to the amino-terminal end of the pesticidal protein. Signal peptides recognized by  E. coli  can be found in proteins already known to be secreted in  E. coli,  for example the OmpA protein (Ghrayeb et al. (1984)  EMBO J,  3:2437-2442). OmpA is a major protein of the  E. coli  outer membrane, and thus its signal peptide is thought to be efficient in the translocation process. Also, the OmpA signal peptide does not need to be modified before processing as may be the case for other signal peptides, for example lipoprotein signal peptide (Duffaud et al. (1987)  Meth. Enzymol.  153: 492). 
     Chimeric pesticidal polypeptides of the invention can be fermented in a bacterial host and the resulting bacteria processed and used as a microbial spray in the same manner that  Bacillus thuringiensis  strains have been used as insecticidal sprays. In the case of a chimeric pesticidal polypeptide(s) that is secreted from  Bacillus,  the secretion signal is removed or mutated using procedures known in the art. Such mutations and/or deletions prevent secretion of the chimeric pesticidal polypeptide(s) into the growth medium during the fermentation process. The chimeric pesticidal polypeptides are retained within the cell, and the cells are then processed to yield the encapsulated chimeric pesticidal polypeptides. Any suitable microorganism can be used for this purpose.  Pseudomonas  has been used to express  Bacillus thuringiensis  endotoxins as encapsulated proteins and the resulting cells processed and sprayed as an insecticide (Gaertner et al. (1993), in:  Advanced Engineered Pesticides,  ed. Kim). 
     Alternatively, the chimeric pesticidal polypeptides are produced by introducing a heterologous nucleic acid encoding the chimeric pesticidal polypeptide into a cellular host. Expression of the heterologous nucleic acid results, directly or indirectly, in the intracellular production and maintenance of the pesticide. These cells are then treated under conditions that prolong the activity of the toxin produced in the cell when the cell is applied to the environment of target pest(s). The resulting product retains the toxicity of the toxin. These naturally encapsulated chimeric pesticidal polypeptides may then be formulated in accordance with conventional techniques for application to the environment hosting a target pest, e.g., soil, water, and foliage of plants. See, for example EPA 0192319, and the references cited therein. 
     The novel chimeric pesticidal polypeptide-encoding polynucleotides and chimeric pesticidal polypeptides can be introduced into plant cells or plants to produce a transgenic plant that is resistant to pests that are susceptible to the chimeric pesticidal polypeptide. 
     The novel chimeric pesticidal polypeptide-encoding polynucleotides can be combined with constitutive, tissue-preferred or other promoters for expression in plants. The promoters can be selected based on the desired outcome. 
     Such constitutive promoters include, for example, the core promoter of the Rsyn7 promoter and other constitutive promoters disclosed in WO 99/43838 and U.S. Pat. No. 6,072,050; the core CaMV 35S promoter (Odell et al. (1985)  Nature  313:810-812); rice actin (McElroy et al. (1990)  Plant Cell  2:163-171); ubiquitin (Christensen et al. (1989)  Plant Mol. Biol.  12:619-632 and Christensen et al. (1992)  Plant Mol. Biol.  18:675-689); pEMU (Last et al. (1991)  Theor. Appl. Genet.  81:581-588); MAS (Velten et al. (1984)  EMBO J.  3:2723-2730); ALS promoter (U.S. Pat. No. 5,659,026), and the like. Other constitutive promoters include, for example, U.S. Pat. Nos. 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785; 5,399,680; 5,268,463; 5,608,142; and 6,177,611. 
     Generally, it will be beneficial to express the gene from an inducible promoter, particularly from a pathogen-inducible promoter. Such promoters include those from pathogenesis-related proteins (PR proteins), which are induced following infection by a pathogen; e.g., PR proteins, SAR proteins, beta-1,3-glucanase, chitinase, etc. See, for example, Redolfi et al. (1983)  Neth. J. Plant Pathol.  89:245-254; Uknes et al. (1992)  Plant Cell  4:645-656; and Van Loon (1985)  Plant Mol. Virol.  4:111-116. See also WO 99/43819, herein incorporated by reference. 
     Of interest are promoters that are expressed locally at or near the site of pathogen infection. See, for example, Marineau et al. (1987)  Plant Mol. Biol.  9:335-342; Matton et al. (1989)  Molecular Plant - Microbe Interactions  2:325-331; Somsisch et al. (1986)  Proc. Natl. Acad. Sci. USA  83:2427-2430; Somsisch et al. (1988)  Mol. Gen. Genet.  2:93-98; and Yang (1996)  Proc. Natl. Acad. Sci. USA  93:14972-14977. See also, Chen et al. (1996)  Plant J.  10:955-966; Zhang et al. (1994)  Proc. Natl. Acad. Sci. USA  91:2507-2511; Warner et al. (1993)  Plant J.  3:191-201; Siebertz et al. (1989)  Plant Cell  1:961-968; U.S. Pat. No. 5,750,386 (nematode-inducible); and the references cited therein. Of particular interest is the inducible promoter for the maize PRms gene, whose expression is induced by the pathogen  Fusarium moniliforme  (see, for example, Cordero et al. (1992)  Physiol. Mol. Plant Path.  41:189-200). 
     Additionally, as pathogens find entry into plants through wounds or insect damage, a wound-inducible promoter may be used in the constructions of the invention. Such wound-inducible promoters include potato proteinase inhibitor (pin II) gene (Ryan (1990)  Ann. Rev. Phytopath.  28:425-449; Duan et al. (1996)  Nature Biotechnology  14:494-498); wun1 and wun2, U.S. Pat. No. 5,428,148; win1 and win2 (Stanford et al. (1989)  Mol. Gen. Genet.  215:200-208); systemin (McGurl et al. (1992)  Science  225:1570-1573); WIP1 (Rohmeier et al. (1993)  Plant Mol. Biol.  22:783-792; Eckelkamp et al. (1993)  FEBS Letters  323:73-76); MPI gene (Corderok et al. (1994)  Plant J.  6(2):141-150); and the like, herein incorporated by reference. 
     Chemical-regulated promoters can be used to modulate the expression of a gene in a plant through the application of an exogenous chemical regulator. Depending upon the objective, the promoter may be a chemical-inducible promoter, where application of the chemical induces gene expression or a chemical-repressible promoter, where application of the chemical represses gene expression. Chemical-inducible promoters are known in the art and include, but are not limited to, the maize In2-2 promoter, which is activated by benzenesulfonamide herbicide safeners, the maize GST promoter, which is activated by hydrophobic electrophilic compounds that are used as pre-emergent herbicides, and the tobacco PR-1 a promoter, which is activated by salicylic acid. Other chemical-regulated promoters of interest include steroid-responsive promoters (see, for example, the glucocorticoid-inducible promoter in Schena et al. (1991)  Proc. Natl. Acad. Sci. USA  88:10421-10425 and McNellis et al. (1998)  Plant J.  14(2):247-257) and tetracycline-inducible and tetracycline-repressible promoters (see, for example, Gatz et al. (1991)  Mol. Gen. Genet.  227:229-237, and U.S. Pat. Nos. 5,814,618 and 5,789,156), herein incorporated by reference. 
     Tissue-preferred promoters can be utilized to target enhanced chimeric pesticidal polypeptide expression within a particular plant tissue. Tissue-preferred promoters include Yamamoto et al. (1997)  Plant J.  12(2):255-265; Kawamata et al. (1997)  Plant Cell Physiol.  38(7):792-803; Hansen et al. (1997)  Mol. Gen Genet.  254(3):337-343; Russell et al. (1997)  Transgenic Res.  6(2):157-168; Rinehart et al. (1996)  Plant Physiol.  112(3):1331-1341; Van Camp et al. (1996)  Plant Physiol.  112(2):525-535; Canevascini et al. (1996)  Plant Physiol.  112(2):513-524; Yamamoto et al. (1994)  Plant Cell Physiol.  35(5):773-778; Lam (1994)  Results Probl. Cell Differ.  20:181-196; Orozco et al. (1993)  Plant Mol Biol.  23(6):1129-1138; Matsuoka et al. (1993)  Proc Natl. Acad. Sci. USA  90(20):9586-9590; and Guevara-Garcia et al. (1993)  Plant J.  4(3):495-505. Such promoters can be modified, if necessary, for weak expression. 
     Leaf-preferred promoters are known in the art. See, for example, Yamamoto et al. (1997)  Plant J.  12(2):255-265; Kwon et al. (1994)  Plant Physiol.  105:357-67; Yamamoto et al. (1994)  Plant Cell Physiol.  35(5):773-778; Gotor et al. (1993)  Plant J.  3:509-18; Orozco et al. (1993)  Plant Mol. Biol.  23(6):1129-1138; and Matsuoka et al. (1993)  Proc. Natl. Acad. Sci. USA  90(20):9586-9590. 
     Root-preferred promoters are known and can be selected from the many available from the literature or isolated de novo from various compatible species. See, for example, Hire et al. (1992)  Plant Mol. Biol.  20(2):207-218 (soybean root-specific glutamine synthetase gene); Keller and Baumgartner (1991)  Plant Cell  3(10):1051-1061 (root-specific control element in the GRP 1.8 gene of French bean); Sanger et al. (1990)  Plant Mol. Biol.  14(3):433-443 (root-specific promoter of the mannopine synthase (MAS) gene of  Agrobacterium tumefaciens ); and Miao et al. (1991)  Plant Cell  3(1):11-22 (full-length cDNA clone encoding cytosolic glutamine synthetase (GS), which is expressed in roots and root nodules of soybean). See also Bogusz et al. (1990)  Plant Cell  2(7):633-641, where two root-specific promoters isolated from hemoglobin genes from the nitrogen-fixing nonlegume  Parasponia andersonii  and the related non-nitrogen-fixing nonlegume  Trema tomentosa  are described. The promoters of these genes were linked to a β-glucuronidase reporter gene and introduced into both the nonlegume  Nicotiana tabacum  and the legume  Lotus corniculatus,  and in both instances root-specific promoter activity was preserved. Leach and Aoyagi (1991) describe their analysis of the promoters of the highly expressed roIC and roID root-inducing genes of  Agrobacterium rhizogenes  (see  Plant Science  (Limerick) 79(1):69-76). They concluded that enhancer and tissue-preferred DNA determinants are dissociated in those promoters. Teeri et al. (1989) used gene fusion to lacZ to show that the  Agrobacterium  T-DNA gene encoding octopine synthase is especially active in the epidermis of the root tip and that the TR2′ gene is root specific in the intact plant and stimulated by wounding in leaf tissue, an especially desirable combination of characteristics for use with an insecticidal or larvicidal gene (see  EMBO J.  8(2):343-350). The TR1′ gene, fused to nptII (neomycin phosphotransferase II) showed similar characteristics. Additional root-preferred promoters include the VfENOD-GRP3 gene promoter (Kuster et al. (1995)  Plant Mol. Biol.  29(4):759-772); and roIB promoter (Capana et al. (1994)  Plant Mol. Biol.  25(4):681-691. See also U.S. Pat. Nos. 5,837,876; 5,750,386; 5,633,363; 5,459,252; 5,401,836; 5,110,732; and 5,023,179. 
     “Seed-preferred” promoters include both “seed-specific” promoters (those promoters active during seed development such as promoters of seed storage proteins) as well as “seed-germinating” promoters (those promoters active during seed germination). See Thompson et al. (1989)  BioEssays  10:108, herein incorporated by reference. Such seed-preferred promoters include, but are not limited to, Cim1 (cytokinin-induced message); cZ19B1 (maize 19 kDa zein); milps (myo-inositol-1-phosphate synthase) (see WO 00/11177 and U.S. Pat. No. 6,225,529; herein incorporated by reference). Gamma-zein is an endosperm-specific promoter. Globulin 1 (Glb-1) is a representative embryo-specific promoter. For dicots, seed-specific promoters include, but are not limited to, bean β-phaseolin, napin, β-conglycinin, soybean lectin, cruciferin, and the like. For monocots, seed-specific promoters include, but are not limited to, maize 15 kDa zein, 22 kDa zein, 27 kDa zein, gamma-zein, waxy, shrunken 1, shrunken 2, Globulin 1, etc. See also WO 00/12733, where seed-preferred promoters from end1 and end2 genes are disclosed; herein incorporated by reference. 
     Where low level expression is desired, weak promoters will be used. Generally, by “weak promoter” is intended a promoter that drives expression of a coding sequence at a low level. By low level is intended at levels of about 1/1000 transcripts to about 1/100,000 transcripts to about 1/500,000 transcripts. Alternatively, it is recognized that weak promoters also encompasses promoters that are expressed in only a few cells and not in others to give a total low level of expression. Where a promoter is expressed at unacceptably high levels, portions of the promoter sequence can be deleted or modified to decrease expression levels. 
     Such weak constitutive promoters include, for example, the core promoter of the Rsyn7 promoter (WO 99/43838 and U.S. Pat. No. 6,072,050), the core 35S CaMV promoter, and the like. Other constitutive promoters include, for example, U.S. Pat. Nos. 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785; 5,399,680; 5,268,463; and 5,608,142. See also, U.S. Pat. No. 6,177,611, herein incorporated by reference. 
     The expression cassette can also comprise a selectable marker gene for the selection of transformed cells. Selectable marker genes are utilized for the selection of transformed cells or tissues. Marker genes include genes encoding antibiotic resistance, such as those encoding neomycin phosphotransferase II (NEO) and hygromycin phosphotransferase (HPT), as well as genes conferring resistance to herbicidal compounds, such as glufosinate ammonium, bromoxynil, imidazolinones, and 2,4-dichlorophenoxyacetate (2,4-D). Additional selectable markers include phenotypic markers such as β-galactosidase and fluorescent proteins such as green fluorescent protein (GFP) (Su et al. (2004)  Biotechnol Bioeng  85:610-9 and Fetter et al. (2004)  Plant Cell  16:215-28), cyan florescent protein (CYP) (Bolte et al. (2004)  J. Cell Science  117:943-54 and Kato et al. (2002)  Plant Physiol  129:913-42), and yellow florescent protein (PhiYFP™ from Evrogen, see, Bolte et al. (2004)  J. Cell Science  117:943-54). For additional selectable markers, see generally, Yarranton (1992)  Curr. Opin. Biotech.  3:506-511; Christopherson et al. (1992)  Proc. Natl. Acad. Sci. USA  89:6314-6318; Yao et al. (1992)  Cell  71:63-72; Reznikoff (1992)  Mol. Microbiol.  6:2419-2422; Barkley et al. (1980) in  The Operon,  pp. 177-220; Hu et al. (1987)  Cell  48:555-566; Brown et al. (1987)  Cell  49:603-612; Figge et al. (1988)  Cell  52:713-722; Deuschle et al. (1989)  Proc. Natl. Acad. Ad. USA  86:5400-5404; Fuerst et al. (1989)  Proc. Natl. Acad. Sci. USA  86:2549-2553; Deuschle et al. (1990)  Science  248:480-483; Gossen (1993) Ph.D. Thesis, University of Heidelberg; Reines et al. (1993)  Proc. Natl. Acad. Sci. USA  90:1917-1921; Labow et al. (1990)  Mol. Cell. Biol.  10:3343-3356; Zambretti et al. (1992)  Proc. Natl. Acad. Sci. USA  89:3952-3956; Bairn et al. (1991)  Proc. Natl. Acad. Sci. USA  88:5072-5076; Wyborski et al. (1991)  Nucleic Acids Res.  19:4647-4653; Hillenand-Wissman (1989)  Topics Mol. Struc. Biol.  10:143-162; Degenkolb et al. (1991)  Antimicrob. Agents Chemother.  35:1591-1595; Kleinschnidt et al. (1988)  Biochemistry  27:1094-1104; Bonin (1993) Ph.D. Thesis, University of Heidelberg; Gossen et al. (1992)  Proc. Natl. Acad. Sci. USA  89:5547-5551; Oliva et al. (1992)  Antimicrob. Agents Chemother.  36:913-919; Hlavka et al. (1985)  Handbook of Experimental Pharmacology,  Vol. 78 (Springer-Verlag, Berlin); Gill et al. (1988)  Nature  334:721-724. Such disclosures are herein incorporated by reference. 
     The above list of selectable marker genes is not meant to be limiting. Any selectable marker gene can be used in the present invention. 
     In one embodiment, the polynucleotide of interest is targeted to the chloroplast for expression. In this manner, where the polynucleotide of interest is not directly inserted into the chloroplast, the expression cassette will additionally contain a nucleic acid encoding a transit peptide to direct the gene product of interest to the chloroplasts. Such transit peptides are known in the art. See, for example, Von Heijne et al. (1991)  Plant Mol. Biol. Rep.  9:104-126; Clark et al. (1989)  J. Biol. Chem.  264:17544-17550; Della-Cioppa et al. (1987)  Plant Physiol.  84:965-968; Romer et al. (1993)  Biochem. Biophys. Res. Commun.  196:1414-1421; and Shah et al. (1986)  Science  233:478-481. 
     Chloroplast targeting sequences are known in the art and include the chloroplast small subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) (de Castro Silva Filho et al. (1996)  Plant Mol. Biol.  30:769-780; Schnell et al. (1991)  J. Biol. Chem.  266(5):3335-3342); 5-(enolpyruvyl)shikimate-3-phosphate synthase (EPSPS) (Archer et al. (1990)  J. Bioenerg. Biomemb.  22(6):789-810); tryptophan synthase (Zhao et al. (1995)  J. Biol. Chem.  270(11):6081-6087); plastocyanin (Lawrence et al. (1997)  J. Biol. Chem.  272(33):20357-20363); chorismate synthase (Schmidt et al. (1993)  J. Biol. Chem.  268(36):27447-27457); and the light harvesting chlorophyll a/b binding protein (LHBP) (Lamppa et al. (1988)  J. Biol. Chem.  263:14996-14999). See also Von Heijne et al. (1991)  Plant Mol. Biol. Rep.  9:104-126; Clark et al. (1989)  J. Biol. Chem.  264:17544-17550; Della-Cioppa et al. (1987)  Plant Physiol.  84:965-968; Romer et al. (1993)  Biochem. Biophys. Res. Commun.  196:1414-1421; and Shah et al. (1986)  Science  233:478-481. 
     Methods for transformation of chloroplasts are known in the art. See, for example, Svab et al. (1990)  Proc. Natl. Acad. Sci. USA  87:8526-8530; Svab and Maliga (1993)  Proc. Natl. Acad. Sci. USA  90:913-917; Svab and Maliga (1993)  EMBO J.  12:601-606. The method relies on particle gun delivery of DNA containing a selectable marker and targeting of the DNA to the plastid genome through homologous recombination. Additionally, plastid transformation can be accomplished by transactivation of a silent plastid-borne transgene by tissue-preferred expression of a nuclear-encoded and plastid-directed RNA polymerase. Such a system has been reported in McBride et al. (1994)  Proc. Natl. Acad. Sci. USA  91:7301-7305. 
     The polynucleotides of interest to be targeted to the chloroplast may be optimized for expression in the chloroplast to account for differences in codon usage between the plant nucleus and this organelle. In this manner, the polynucleotide of interest may be synthesized using chloroplast-preferred codons. See, for example, U.S. Pat. No. 5,380,831, herein incorporated by reference. 
     The methods of the invention involve introducing a polypeptide or polynucleotide into a plant or other organism. “Introducing” is intended to mean presenting to the organism the polynucleotide or polypeptide in such a manner that the sequence gains access to the interior of a cell of the organism. The methods of the invention do not depend on a particular method for introducing a sequence into an organism, only that the polynucleotide or polypeptides gains access to the interior of at least one cell of the organism. Methods for introducing polynucleotides or polypeptides into plants are known in the art including, but not limited to, stable transformation methods, transient transformation methods, and virus-mediated methods. 
     “Stable transformation” is intended to mean that the nucleotide construct introduced into a plant integrates into the genome of the plant and is capable of being inherited by the progeny thereof. “Transient transformation” is intended to mean that a polynucleotide is introduced into the plant and does not integrate into the genome of the plant or a polypeptide is introduced into a plant. 
     Transformation protocols as well as protocols for introducing polypeptides or polynucleotide sequences into plants may vary depending on the type of plant or plant cell, i.e., monocot or dicot, targeted for transformation. Suitable methods of introducing polypeptides and polynucleotides into plant cells include microinjection (Crossway et al. (1986)  Biotechniques  4:320-334), electroporation (Riggs et al. (1986)  Proc. Natl. Acad. Sci. USA  83:5602-5606,  Agrobacterium -mediated transformation (U.S. Pat. No. 5,563,055 and U.S. Pat. No. 5,981,840), direct gene transfer (Paszkowski et al. (1984)  EMBO J.  3:2717-2722), and ballistic particle acceleration (see, for example, U.S. Pat. Nos. 4,945,050; U.S. Pat. No. 5,879,918; U.S. Pat. Nos. 5,886,244; and, 5,932,782; Tomes et al. (1995) in  Plant Cell, Tissue, and Organ Culture: Fundamental Methods,  ed. Gamborg and Phillips (Springer-Verlag, Berlin); McCabe et al. (1988)  Biotechnology  6:923-926); and Lec1 transformation (WO 00/28058). Also see Weissinger et al. (1988)  Ann. Rev. Genet.  22:421-477; Sanford et al. (1987)  Particulate Science and Technology  5:27-37 (onion); Christou et al. (1988)  Plant Physiol.  87:671-674 (soybean); McCabe et al. (1988)  Bio/Technology  6:923-926 (soybean); Finer and McMullen (1991)  In Vitro Cell Dev. Biol.  27P:175-182 (soybean); Singh et al. (1998)  Theor. Appl. Genet.  96:319-324 (soybean); Datta et al. (1990)  Biotechnology  8:736-740 (rice); Klein et al. (1988)  Proc. Natl. Acad. Sci. USA  85:4305-4309 (maize); Klein et al. (1988)  Biotechnology  6:559-563 (maize); U.S. Pat. Nos. 5,240,855; 5,322,783; and, 5,324,646; Klein et al. (1988)  Plant Physiol.  91:440-444 (maize); Fromm et al. (1990)  Biotechnology  8:833-839 (maize); Hooykaas-Van Slogteren et al. (1984)  Nature  ( London ) 311:763-764; U.S. Pat. No. 5,736,369 (cereals); Bytebier et al. (1987)  Proc. Natl. Acad. Sci. USA  84:5345-5349 (Liliaceae); De Wet et al. (1985) in  The Experimental Manipulation of Ovule Tissues,  ed. Chapman et al. (Longman, New York), pp. 197-209 (pollen); Kaeppler et al. (1990)  Plant Cell Reports  9:415-418 and Kaeppler et al. (1992)  Theor. Appl. Genet.  84:560-566 (whisker-mediated transformation); D&#39;Halluin et al. (1992)  Plant Cell  4:1495-1505 (electroporation); Li et al. (1993)  Plant Cell Reports  12:250-255 and Christou and Ford (1995)  Annals of Botany  75:407-413 (rice); Osjoda et al. (1996)  Nature Biotechnology  14:745-750 (maize via  Agrobacterium tumefaciens ); all of which are herein incorporated by reference. 
     In specific embodiments, the chimeric pesticidal polypeptide-encoding sequences of the invention can be provided to a plant using a variety of transient transformation methods. Such transient transformation methods include, but are not limited to, the introduction of the pesticidal protein or variants and fragments thereof directly into the plant or the introduction of the a pesticide-encoding transcript into the plant. Such methods include, for example, microinjection or particle bombardment. See, for example, Crossway et al. (1986)  Mol Gen. Genet.  202:179-185; Nomura et al. (1986)  Plant Sci.  44:53-58; Hepler et al. (1994)  Proc. Natl. Acad. Sci.  91: 2176-2180 and Hush et al. (1994)  The Journal of Cell Science  107:775-784, all of which are herein incorporated by reference. Alternatively, the pesticide-encoding polynucleotide can be transiently transformed into the plant using techniques known in the art. Such techniques include a viral vector system and the precipitation of the polynucleotide in a manner that precludes subsequent release of the DNA. Thus, the transcription from the particle-bound DNA can occur, but the frequency with which it is released to become integrated into the genome is greatly reduced. Such methods include the use of particles coated with polyethylimine. 
     In other embodiments, the novel chimeric pesticidal polypeptide-encoding polynucleotide may be introduced into plants by contacting plants with a virus or viral nucleic acids. Generally, such methods involve incorporating a nucleotide construct within a viral DNA or RNA molecule. Methods for introducing polynucleotides into plants and expressing a protein encoded therein, involving viral DNA or RNA molecules, are known in the art. See, for example, U.S. Pat. Nos. 5,889,191, 5,889,190, 5,866,785, 5,589,367, 5,316,931, and Porta et al. (1996)  Molecular Biotechnology  5:209-221; herein incorporated by reference. 
     The cells that have been transformed may be grown into plants in accordance with conventional ways. See, for example, McCormick et al. (1986)  Plant Cell Reports  5:81-84. These plants may then be grown, and either pollinated with the same transformed strain or different strains, and the resulting progeny having constitutive expression of the desired phenotypic characteristic identified. Two or more generations may be grown to ensure that expression of the desired phenotypic characteristic is stably maintained and inherited and then seeds harvested to ensure expression of the desired phenotypic characteristic has been achieved. In this manner, the present invention provides transformed seed (also referred to as “transgenic seed”) having a polynucleotide of the invention, for example, an expression cassette of the invention, stably incorporated into their genome. 
     As used herein, the term plant also includes plant cells, plant protoplasts, plant cell tissue cultures from which plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants such as embryos, pollen, ovules, seeds, leaves, flowers, branches, fruit, kernels, ears, cobs, husks, stalks, roots, root tips, anthers, and the like. Grain is intended to mean the mature seed produced by commercial growers for purposes other than growing or reproducing the species. Progeny, variants, and mutants of the regenerated plants are also included within the scope of the invention, provided that these parts comprise the introduced polynucleotides. 
     The present invention may be used for transformation of any plant species, including, but not limited to, monocots and dicots. Examples of plant species of interest include, but are not limited to, corn ( Zea mays ),  Brassica  sp. (e.g.,  B. napus, B. rapa, B. juncea ), particularly those  Brassica  species useful as sources of seed oil, alfalfa ( Medicago sativa ), rice ( Oryza sativa ), rye ( Secale cereale ), sorghum ( Sorghum bicolor, Sorghum vulgare ), millet (e.g., pearl millet ( Pennisetum glaucum ), proso millet ( Panicum miliaceum ), foxtail millet ( Setaria italica ), finger millet ( Eleusine coracana )), sunflower ( Helianthus annuus ), safflower ( Carthamus tinctorius ), wheat ( Triticum aestivum ), soybean ( Glycine max ), tobacco ( Nicotiana tabacum ), potato ( Solanum tuberosum ), peanuts ( Arachis hypogaea ), cotton ( Gossypium barbadense, Gossypium hirsutum ), sweet potato ( Ipomoea batatus ), cassava ( Manihot esculenta ), coffee ( Coffea  spp.), coconut ( Cocos nucifera ), pineapple ( Ananas comosus ), citrus trees ( Citrus  spp.), cocoa ( Theobroma cacao ), tea ( Camellia sinensis ), banana ( Musa  spp.), avocado ( Persea americana ), fig ( Ficus casica ), guava ( Psidium guajava ), mango ( Mangifera indica ), olive ( Olea europaea ), papaya ( Carica papaya ), cashew ( Anacardium occidentale ), macadamia ( Macadamia integrifolia ), almond ( Prunus amygdalus ), sugar beets ( Beta vulgaris ), sugarcane ( Saccharum  spp.), oats, barley, vegetables ornamentals, and conifers. 
     Vegetables include tomatoes ( Lycopersicon esculentum ), lettuce (e.g.,  Lactuca sativa ), green beans ( Phaseolus vulgaris ), lima beans ( Phaseolus limensis ), peas ( Lathyrus  spp.), and members of the genus  Cucumis  such as cucumber ( C. sativus ), cantaloupe ( C. cantalupensis ), and musk melon ( C. melo ). Ornamentals include azalea ( Rhododendron  spp.), hydrangea ( Macrophylla hydrangea ), hibiscus ( Hibiscus rosasanensis ), roses ( Rosa  spp.), tulips ( Tulipa  spp.), daffodils ( Narcissus  spp.), petunias ( Petunia hybrida ), carnation ( Dianthus caryophyllus ), poinsettia ( Euphorbia pulcherrima ), and chrysanthemum. 
     Conifers that may be employed in practicing the present invention include, for example, pines such as loblolly pine ( Pinus taeda ), slash pine ( Pinus elliotii ), ponderosa pine ( Pinus ponderosa ), lodgepole pine ( Pinus contorta ), and Monterey pine ( Pinus radiata ); Douglas-fir ( Pseudotsuga menziesii ); Western hemlock ( Tsuga canadensis ); Sitka spruce ( Picea glauca ); redwood ( Sequoia sempervirens ); true firs such as silver fir ( Abies amabilis ) and balsam fir ( Abies balsamea ); and cedars such as Western red cedar ( Thuja plicata ) and Alaska yellow-cedar ( Chamaecyparis nootkatensis ). In specific embodiments, plants of the present invention are crop plants (for example, corn, alfalfa, sunflower,  Brassica,  soybean, cotton, safflower, peanut, sorghum, wheat, millet, tobacco, etc.). In other embodiments, corn and soybean and sugarcane plants are optimal, and in yet other embodiments corn plants are optimal. 
     Other plants of interest include grain plants that provide seeds of interest, oil-seed plants, and leguminous plants. Seeds of interest include grain seeds, such as corn, wheat, barley, rice, sorghum, rye, etc. Oil-seed plants include cotton, soybean, safflower, sunflower,  Brassica,  maize, alfalfa, palm, coconut, etc. Leguminous plants include beans and peas. Beans include guar, locust bean, fenugreek, soybean, garden beans, cowpea, mungbean, lima bean, fava bean, lentils, chickpea, etc. 
     The present invention also provides a pesticidal composition comprising an effective amount of at least one chimeric pesticidal polypeptide of the invention to reduce pest damage to the plant and methods of protecting a plant from a pest involving providing to the plant or to the vicinity of the plant an effective amount of a pesticidal composition. By “effective amount” and “effective concentration” is intended an amount and concentration, respectively, that is sufficient to kill or inhibit the growth of a plant pest. Methods for determining an “effective amount” and “effective concentration” are known to those of ordinary skill in art and include, for example, assays involving placing varying amounts of concentrations of the active ingredient (e.g., a chimeric pesticidal polypeptide) in contact with and/or in the vicinity of a pest and monitoring the survival and/or growth of the pest over time. 
     As described above, the effective amount of a chimeric pesticidal polypeptide can vary depending on the formulation and method in which the formulation is applied to the plant or plant environment. As such, bacteria can be transformed with a nucleotide sequence encoding a chimeric pesticidal polypeptide and can be used in the pesticidal compositions as described herein. Thus, the pesticidal composition can be an organism that is transformed to express a chimeric pesticidal polypeptide. Alternatively, a chimeric pesticidal polypeptide can be purified from the bacteria as described above. In some embodiments, the pesticidal composition or pesticidal formulation can include other agricultural protectants, as described above. As used herein, the term “pesticidal composition” and “pesticidial formulation” are equivalent terms that have the same meaning unless indicated otherwise or apparent from the context as used. 
     As described above, the effective amount of a chimeric pesticidal polypeptide can vary depending on the formulation and method in which the formulation is applied to the plant or plant environment. As such, bacteria can be transformed with a nucleotide sequence encoding a chimeric pesticidal polypeptide and can be used in the pesticidal compositions as described herein. Thus, the pesticidal composition can be an organism that is transformed to express a chimeric pesticidal polypeptide. Alternatively, the chimeric pesticidal polypeptide can be purified from the bacteria as described above. In some embodiments, the pesticidal compositions or pesticidal formulation can include other agricultural protectants, as described above. 
     As described above, the pesticidal composition can be, for example, a dust, emulsion, solid (e.g., particle or pellets) or liquid. 
     The pesticidal composition can be provided to the plant by applying the pesticidal composition directly to the environment of the plant or to the vicinity of the plant such as, for example, in the soil or other growth medium surrounding the plant to protect the plant from pest attacks. For example, the pesticidal composition can be applied directly to the plant by atomizing, broadcasting, coating or pouring, dusting spraying, scattering, soil drenching, sprinkling or seed coating at the time when the pest such as, for example, an insect pest has begun to appear on the plant or before the appearance of insect pests as a protective measure. 
     Alternatively, the pesticidal composition can be introduced into irrigation water and then applied to the plant during watering. It is preferred to obtain good control of pest in the early stages of plant growth as this is the time when the plant can be most severely damaged. To maintain protection as plants grow and to obtain the greatest protection of large plants, repeated applications of the pesticidal composition can be beneficial. 
     The number of applications and the rate of application depend on the intensity of infestation by the corresponding pest. When the pesticidal composition not only includes the chimeric pesticidal polypeptide and/or a chimeric pesticidal polypeptide-expressing bacteria, but also includes another agricultural protectant, the formulation can be applied to the crop area or plant to be treated simultaneously or in succession (i.e., sequentially). 
     The pesticidal composition will reduce pest-related damage by at least about 5% to about 50%, at least about 10% to about 60%, at least about 30% to about 70%, at least about 40% to about 80% or at least about 50% to about 90% or greater. Hence, the methods can be utilized to protect plants from pests. Protection may vary from a slight decrease in plant damage caused by the pest (e.g., partial inhibition) to total decrease such that the plant is unaffected by the presence of the pest. 
     The present invention therefore provides methods of protecting plants, plant parts and plant host cells by providing a pesticidal composition comprising a chimeric pesticidal polypeptide. 
     In certain embodiments the polynucleotides of the present invention can be stacked with any combination of polynucleotide sequences of interest in order to create plants with a desired trait. A trait, as used herein, refers to the phenotype derived from a particular sequence or groups of sequences. For example, the polynucleotides of the present invention may be stacked with any other polynucleotides encoding polypeptides having pesticidal and/or insecticidal activity, such as other  Bacillus thuringiensis  toxic proteins (described in U.S. Pat. Nos. 5,366,892; 5,747,450; 5,737,514; 5,723,756; 5,593,881; and Geiser et al. (1986)  Gene  48:109), lectins (Van Damme et al. (1994)  Plant Mol. Biol.  24:825, pentin (described in U.S. Pat. No. 5,981,722), and the like. The combinations generated can also include multiple copies of any one of the polynucleotides of interest. The polynucleotides of the present invention can also be stacked with any other gene or combination of genes to produce plants with a variety of desired trait combinations including, but not limited to, traits desirable for animal feed such as high oil genes (e.g., U.S. Pat. No. 6,232,529); balanced amino acids (e.g., hordothionins (U.S. Pat. Nos. 5,990,389; 5,885,801; 5,885,802; and 5,703,409); barley high lysine (Williamson et al. (1987)  Eur. J. Biochem.  165:99-106; and WO 98/20122) and high methionine proteins (Pedersen et al. (1986)  J. Biol. Chem.  261:6279; Kirihara et al. (1988)  Gene  71:359; and Musumura et al. (1989)  Plant Mol. Biol.  12:123)); increased digestibility (e.g., modified storage proteins (U.S. application Ser. No. 10/053,410, filed Nov. 7, 2001); and thioredoxins (U.S. application Ser. No. 10/005,429, filed Dec. 3, 2001)); the disclosures of which are herein incorporated by reference. 
     The polynucleotides of the present invention can also be stacked with traits desirable for disease or herbicide resistance (e.g., fumonisin detoxification genes, U.S. Pat. No. 5,792,931); avirulence and disease resistance genes (Jones et al. (1994)  Science  266:789; Martin et al. (1993)  Science  262:1432; Mindrinos et al. (1994)  Cell  78:1089); acetolactate synthase (ALS) mutants that lead to herbicide resistance such as the S4 and/or Hra mutations; inhibitors of glutamine synthase such as phosphinothricin or basta (e.g., bar gene); and glyphosate resistance (EPSPS gene); and traits desirable for processing or process products such as high oil (e.g., U.S. Pat. No. 6,232,529); modified oils (e.g., fatty acid desaturase genes (U.S. Pat. No. 5,952,544; WO 94/11516)); modified starches (e.g., ADPG pyrophosphorylases (AGPase), starch synthases (SS), starch branching enzymes (SBE), and starch debranching enzymes (SDBE)); and polymers or bioplastics (e.g., U.S. Pat. No. 5.602,321; beta-ketothiolase, polyhydroxybutyrate synthase, and acetoacetyl-CoA reductase (Schubert et al. (1988)  J. Bacteriol.  170:5837-5847) facilitate expression of polyhydroxyalkanoates (PHAs)); the disclosures of which are herein incorporated by reference. One could also combine the polynucleotides of the present invention with polynucleotides providing agronomic traits such as male sterility (e.g., see U.S. Pat. No. 5.583,210), stalk strength, flowering time or transformation technology traits such as cell cycle regulation or gene targeting (e.g., WO 99/61619, WO 00/17364, and WO 99/25821); the disclosures of which are herein incorporated by reference. 
     These stacked combinations can be created by any method including, but not limited to, cross-breeding plants by any conventional or TopCross methodology or genetic transformation. If the sequences are stacked by genetically transforming the plants, the polynucleotide sequences of interest can be combined at any time and in any order. For example, a transgenic plant comprising one or more desired traits can be used as the target to introduce further traits by subsequent transformation. The traits can be introduced simultaneously in a co-transformation protocol with the polynucleotides of interest provided by any combination of transformation cassettes. For example, if two sequences will be introduced, the two sequences can be contained in separate transformation cassettes (trans) or contained on the same transformation cassette (cis). Expression of the sequences can be driven by the same promoter or by different promoters. In certain cases, it may be desirable to introduce a transformation cassette that will suppress the expression of the polynucleotide of interest. This may be combined with any combination of other suppression cassettes or overexpression cassettes to generate the desired combination of traits in the plant. It is further recognized that polynucleotide sequences can be stacked at a desired genomic location using a site-specific recombination system. See, for example, WO99/25821, WO99/25854, WO99/25840, WO99/25855, and WO99/25853, all of which are herein incorporated by reference. 
     Various changes in phenotype are of interest including modifying the fatty acid composition in a plant, altering the amino acid content of a plant, altering a plant&#39;s pathogen defense mechanism, and the like. These results can be achieved by providing expression of heterologous products or increased expression of endogenous products in plants. Alternatively, the results can be achieved by providing for a reduction of expression of one or more endogenous products, particularly enzymes or cofactors in the plant. These changes result in a change in phenotype of the transformed plant. 
     Genes of interest are reflective of the commercial markets and interests of those involved in the development of the crop. Crops and markets of interest change, and as developing nations open up world markets, new crops and technologies will emerge also. In addition, as our understanding of agronomic traits and characteristics such as yield and heterosis increase, the choice of genes for transformation will change accordingly. General categories of genes of interest include, for example, those genes involved in information, such as zinc fingers, those involved in communication, such as kinases, and those involved in housekeeping, such as heat shock proteins. More specific categories of transgenes, for example, include genes encoding important traits for agronomics, insect resistance, disease resistance, herbicide resistance, sterility, grain characteristics, and commercial products. Genes of interest include, generally, those involved in oil, starch, carbohydrate or nutrient metabolism as well as those affecting kernel size, sucrose loading, and the like. 
     Agronomically important traits such as oil, starch, and protein content can be genetically altered in addition to using traditional breeding methods. Modifications include increasing content of oleic acid, saturated and unsaturated oils, increasing levels of lysine and sulfur, providing essential amino acids, and also modification of starch. Hordothionin protein modifications are described in U.S. Pat. Nos. 5,703,049, 5,885,801, 5,885,802, and 5,990,389, herein incorporated by reference. Another example is lysine and/or sulfur rich seed protein encoded by the soybean 2S albumin described in U.S. Pat. No. 5,850,016, and the chymotrypsin inhibitor from barley, described in Williamson et al. (1987)  Eur. J. Biochem.  165:99-106, the disclosures of which are herein incorporated by reference. 
     Derivatives of the coding sequences can be made by site-directed mutagenesis to increase the level of preselected amino acids in the encoded polypeptide. For example, the gene encoding the barley high lysine polypeptide (BHL) is derived from barley chymotrypsin inhibitor, U.S. application Ser. No. 08/740,682, filed Nov. 1, 1996, and WO 98/20133, the disclosures of which are herein incorporated by reference. Other proteins include methionine-rich plant proteins such as from sunflower seed (Lilley et al. (1989)  Proceedings of the World Congress on Vegetable Protein Utilization in Human Foods and Animal Feedstuffs,  ed. Applewhite (American Oil Chemists Society, Champaign, Ill.), pp. 497-502; herein incorporated by reference); corn (Pedersen et al. (1986)  J. Biol. Chem.  261:6279; Kirihara et al. (1988)  Gene  71:359; both of which are herein incorporated by reference); and rice (Musumura et al. (1989)  Plant Mol. Biol.  12:123, herein incorporated by reference). Other agronomically important genes encode latex, Floury 2, growth factors, seed storage factors, and transcription factors. 
     Insect resistance genes may encode resistance to pests that have great yield drag such as rootworm, cutworm, European Corn Borer, and the like. Such genes include, for example,  Bacillus thuringiensis  toxic protein genes (U.S. Pat. Nos. 5,366,892; 5,747,450; 5,736,514; 5,723,756; 5,593,881; and Geiser et al. (1986)  Gene  48:109); and the like. 
     Genes encoding disease resistance traits include detoxification genes, such as against fumonosin (U.S. Pat. No. 5,792,931); avirulence (avr) and disease resistance (R) genes (Jones et al. (1994)  Science  266:789; Martin et al. (1993)  Science  262:1432; and Mindrinos et al. (1994)  Cell  78:1089); and the like. 
     Herbicide resistance traits may include genes coding for resistance to herbicides that act to inhibit the action of acetolactate synthase (ALS), in particular the sulfonylurea-type herbicides (e.g., the acetolactate synthase (ALS) gene containing mutations leading to such resistance, in particular the S4 and/or Hra mutations), genes coding for resistance to herbicides that act to inhibit action of glutamine synthase, such as phosphinothricin or basta (e.g., the bar gene); glyphosate (e.g., the EPSPS gene and the GAT gene; see, for example, U.S. Publication No. 20040082770 and WO 03/092360); or other such genes known in the art. The bar gene encodes resistance to the herbicide basta, the nptII gene encodes resistance to the antibiotics kanamycin and geneticin, and the ALS-gene mutants encode resistance to the herbicide chlorsulfuron. 
     Sterility genes can also be encoded in an expression cassette and provide an alternative to physical detasseling. Examples of genes used in such ways include male tissue-preferred genes and genes with male sterility phenotypes such as QM, described in U.S. Pat. No. 5,583,210. Other genes include kinases and those encoding compounds toxic to either male or female gametophytic development. 
     The quality of grain is reflected in traits such as levels and types of oils, saturated and unsaturated, quality and quantity of essential amino acids, and levels of cellulose. In corn, modified hordothionin proteins are described in U.S. Pat. Nos. 5,703,049, 5,885,801, 5,885,802, and 5,990,389. 
     Commercial traits can also be encoded on a gene or genes that could increase for example, starch for ethanol production or provide expression of proteins. Another important commercial use of transformed plants is the production of polymers and bioplastics such as described in U.S. Pat. No. 5,602,321. Genes such as β-Ketothiolase, PHBase (polyhydroxyburyrate synthase), and acetoacetyl-CoA reductase (see Schubert et al. (1988)  J. Bacteriol.  170:5837-5847) facilitate expression of polyhyroxyalkanoates (PHAs). 
     Exogenous products include plant enzymes and products as well as those from other sources including prokaryotes and other eukaryotes. Such products include enzymes, cofactors, hormones, and the like. The level of proteins, particularly modified proteins having improved amino acid distribution to improve the nutrient value of the plant, can be increased. This is achieved by the expression of such proteins having enhanced amino acid content. 
     Throughout this specification and the claims, the words “comprise,” “comprises,” and “comprising” are used in a non-exclusive sense, except where the context requires otherwise. 
     As used herein, the term “about,” when referring to a value is meant to encompass variations of, in some embodiments ±50%, in some embodiments ±20%, in some embodiments ±10%, in some embodiments ±5%, in some embodiments ±1%, in some embodiments ±0.5%, and in some embodiments ±0.1% from the specified amount, as such variations are appropriate to perform the disclosed methods or employ the disclosed compositions. 
     Further, when an amount, concentration or other value or parameter is given as either a range, preferred range or a list of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. Where a range of numerical values is recited herein, unless otherwise stated, the range is intended to include the endpoints thereof, and all integers and fractions within the range. It is not intended that the scope of the presently disclosed subject matter be limited to the specific values recited when defining a range. 
     The following examples are offered by way of illustration and not by way of limitation. 
     EXAMPLE 1 
     Enhancement of the Insecticidal Activity of Cry Proteins Against Western Corn Rootworms and Black Cutworms When Fused to MBP 
     A computer-designed and artificially synthesized Cry3Aa-type protein called IP3-1 (SEQ ID NO: 8), a truncated RX002 protein (SEQ ID NO: 12) cloned from a Bt strain, a shuffled Cry8Bb-type protein called 2A-12 (SEQ ID NO: 10), and a truncated native Cry1Bd protein called 4c6 (SEQ ID NO: 14) were expressed in  E. coli  BL21 as fusion proteins comprising an operably linked MBP. The amino acid sequences of the MBP-IP3-1, MBP-RX002, MBP-2A-12, and MBP-4c6 fusion proteins are set forth in SEQ ID NOS: 21, 23, 25, and 27, respectively. Nucleotide sequences encoding the MBP-IP3-1, MBP-RX002, MBP-2A-12, and MBP-4c6 fusion proteins are set forth in SEQ ID NOS: 20, 22, 24, and 26, respectively. 
     To express MBP with these Bt Cry proteins, corresponding genes were cloned in an NEB pMAL vector and the proteins were purified using amylose resin following the manufacturer&#39;s recommended method (New England Biolabs, Inc., Ipswich, Mass., USA; Catalog No. E8021L). The target protein eluted from amylase resin was concentrated in Amicon Centricon concentrator (10 kDa cutoff). The final fusion proteins were dissolved in 25 mM HEPES-NaOH buffer pH8 at a concentration around 2 mg/ml. The protein concentration was determined by SDS-PAGE using bovine serum albumin as the reference. The pMAL vector has a protease site specific to Factor Xa that cleaves MBP and the linker off the Cry protein. All Cry proteins included in this application were resistant to Factor Xa. The protease digestion was carried out in the HEPES buffer at 1:25 protease and substrate ration at 20° C. for 16 hr. The digestion leading to the complete MBP removal was confirmed by SDS-PAGE. 
     MBP fusion and MBP free Cry proteins were diluted in 25 mM HEPES-NaOH buffer pH 8 and 10 μL of diluted samples were mixed with 40 μL of molten artificial insect diet made with low temperature melting agarose. The diet mixture was then placed in each well of a 96-well micro-titer plate and allowed to feed with neonate insect larvae. After 4 days at 27° C., the responses of insects towards the Cry proteins were scored using a 0-3 numerical scoring system based on the size and mortality. If no response or normal growth was seen, Score 0 was given. When the growth was somewhat retarded without any mortality, it was Score 1. Score 2 meant partial death (multiple insects were used in each well) and strong growth inhibition. Score 3 indicated the complete mortality. Each treatment was repeated 6 times for possible highest score of 18 (3×6). In this scoring system, Score 9 with 6 repeats of one treatment means the 50% response (9 out of 18) of the treatment and called ILC50 (growth Inhibition and Lethal Concentration for 50% response). The results of these assays are shown in Tables 3 and 4. 
     The results shown in Tables 3 and 4 with four different Cry proteins demonstrate that MBP-Cry fusion proteins have significantly increased insecticidal activity against both Coleopteran (Western Corn Rootworm) and Lepidopteran (Black Cutworm) insect species, when compared to the insecticidial activity of their respective free Cry proteins. These results further demonstrate the general applicability of the methods of the present invention to enhancing the insecticidal activity of Cry proteins. Table 3 shows ILC50 (based on the size or weight of the Cry protein portion) values for insecticidal activity against Western Corn Rootworm of MBP-Cry Fusion Proteins and Free Cry Proteins. 
     
       
         
           
               
               
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 IP3-1 
                 RX002 
                 2A-12 
               
            
           
           
               
               
               
               
               
               
            
               
                 MBP- 
                 free  
                 MBP- 
                 free  
                 MBP- 
                 free  
               
               
                 fusion 
                 Cry 
                 fusion 
                 Cry 
                 fusion 
                 Cry 
               
               
                   
               
               
                 7 ppm 
                 103 ppm 
                 89 ppm 
                 No activity* 
                 32 ppm 
                 152 ppm 
               
               
                   
               
               
                 *Only an 11% response was observed at 2480 ppm. 
               
            
           
         
       
     
     Table 4 shows the ILC50 values for insecticidal activity against Black Cutworm of a MBP-4c6 Cry Fusion Protein and Free 4c6 Cry Protein. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 4 
               
               
                   
                   
               
               
                   
                 MBP-4c6 fusion 
                 free 4c6 
               
               
                   
                   
               
             
            
               
                   
                 75 ppm 
                 148 ppm 
               
               
                   
                   
               
            
           
         
       
     
     EXAMPLE 2 
     MBP-Cry Fusion Protein With Increased Activity Against WCRW 
     A new vector, pMAL-SA (SEQ ID NO: 19), was constructed for making MBP-Cry fusion proteins. This vector is based on NEB pMAL vector. It has the cry gene cloning site delineated with SphI and BamHI recognition sequences at the end of MBP and a specially designed linker called “SA” linker between MBP and the Cry cloning site. 
     In order to clone a Bt cry gene, the cry coding region is amplified by PCR using appropriate forward and reverse primers. In the forward primer, there is an SphI site over the ATG translation initiation site. In the reverse primer, there is a stop codon at the end of the cry gene coding region and a BamHI site. 
     A computer-designed cry3Aa sequence, IP3-1, was synthesized and used as the PCR template. The IP3-1 nucleotide sequence is set forth in SEQ ID NO: 7. This IP3-1 gene was cloned in pMAL-SA as described above to produce a plasmid that contains MBP, the SA linker and IP3-1 coding region (SEQ ID NO: 1). The second amino acid residue of IP3-1 was mutated back to Asn from His to produce the MBP-SA-IP3-1 nucleotide sequence set forth in SEQ ID NO: 2. 
     The MBP-SA-IP3-1 fusion protein was expressed in  E. coli  BL21 and purified by amylose resin affinity chromatography according to the method described in Example 1. The column eluate was concentrated in Amicon Centricon and its buffer was exchanged to 25 mM HEPES-NaOH buffer pH 8. The fusion protein was digested with trypsin at 1:25 trypsin-substrate ratio at 37° C. for 1 hr. Trypsin cleaves the protein at the end of the linker to liberate the Cry protein from MBP and the SA linker. IP3-1 was resistant to trypsin under this digestion condition. The digestion was confirmed with SDS-PAGE. Both the fusion and MBP-free IP3-1 proteins were assayed against WCRW. Table 5 shows ILC50 values for insecticidal activity against Western Corn Rootworm of a MBP-SA-IP3-1 and MBP-free IP3-1. The assay results in Table 5 demonstrate that there is a significant enhancement of WCRW activity when IP3-1 is operable linked to MBP-SA (i.e., MBP-SA-IP3-1), when compared to the insecticidal activity of MBP-free IP3-1 against WCRW. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 5 
               
               
                   
                   
               
               
                   
                 Protein 
                 ICL50 
               
               
                   
                   
               
             
            
               
                   
                 MBP-SA-IP3-1 
                  6 ppm 
               
               
                   
                 MBP-free IP3-1 
                 269 ppm 
               
               
                   
                   
               
            
           
         
       
     
     EXAMPLE 3 
     Enhancement of Cry3Aa Protein Activity When Fused to NusA and Trx 
     A computer-designed and artificially synthesized cry3Aa-type gene called IP3-1 was cloned in pET-43.1 EK/LIC for NusA fusion and pET-32 for TrxA fusion by following the manufacturer&#39;s directions (EMD Biosciences, Madison, Wis., USA). These vectors were used to express NusA-IP3-1 and TrxA-IP3-1 fusion proteins. The amino acid sequences of the NusA-IP3-1 and TrxA-IP3-1 fusion proteins are set forth in SEQ ID NOS: 16 and 18, respectively. Nucleotide sequences encoding the NusA-IP3-1 and TrxA-IP3-1 fusion proteins are set forth in SEQ ID NOS: 15 and 17, respectively. The amino acid sequence of IP3-1 is set forth in SEQ ID NO: 8. A nucleotide sequence encoding IP3-1 is set forth in SEQ ID NO: 7. 
     The fusion proteins were purified by affinity chromatography using Ni-NTA agarose (Qiagen Inc., Valencia, Calif., USA) according to the manufacturer&#39;s directions. The Cry3Aa protein was then digested away from the tags including NusA, TrxA, 6XHis etc. with enterokinase and the insecticidal activity of the free Cry3A protein was compared with its NusA and TrxA fusion proteins. The insect assay was conducted using WCRW as described above. Table 6 shows the ILC50 values for insecticidal activity against Western Corn Rootworm of a MBP-SA-IP3-1 and MBP-free IP3-1 protein. The IP3-1 protein sample was produced from the NusA-Cry3Aa fusion by enterokinase digestion. As demonstrated in the results in Table 6, both the NusA-IP3-1-and TrxA-IP3-1-fusion proteins, which comprise a solubility-enhancing polypeptide and a Cry3A protein (IP3-1), have an increased insecticidal activity against WCRW as evidenced by the significantly lower ICL50 values, when compared to insecticidal activity of free IP3-1. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 6 
               
               
                   
                   
               
               
                   
                 Protein 
                 ICL50 
               
               
                   
                   
               
             
            
               
                   
                 NusA-IP3-1-fusion 
                  26 ppm 
               
               
                   
                 TrxA-IP3-1-fusion 
                  31 ppm 
               
               
                   
                 Tag free IP3-1 
                 554 ppm 
               
               
                   
                   
               
            
           
         
       
     
     EXAMPLE 4 
     Generation of a MBP-SA-RX002 Transformation Construct 
     The maltose-binding protein (MBP-SA) (SEQ ID NO: 4) was fused to the N-terminus of RX002 (SEQ ID NO:11) in a synthetic gene designed for this invention (SEQ ID NO:32 encoding SEQ ID NO:33). The gene was cloned as a BamHI—Stul fragment into a Gateway entry vector containing a plant expression cassette with the BSV(AY) TR PROMOTER—ADH1 INTRON1 sequence and the potato PIN II terminator sequence. The resulting plant expression cassette contains the following components operatively linked together in this order; BSV (AY) TR PRO-ADH1-INTRON1, the MBP-SA-RX002 gene, and the PIN II terminator. The expression cassette is flanked by Gateway attL3 and attL4 recombination sites and this entry vector was used to transfer the expression cassette into an attR3 and attR4 containing binary destination transformation vector. The final transformation vector contains the MBP-SA-RX002 expression cassette upstream of a cassette containing the maize Ubiquitin1 promoter-5′UTR-Ubiquitin intron1 controlling expression of a PAT selectable marker gene with the 35S terminator sequence. 
     EXAMPLE 5 
       Agrobacterium -Mediated Transformation of Maizeand Regeneration of Transgenic Plants 
     For  Agrobacterium -mediated transformation of maize with a promoter sequence of the invention, the method of Zhao was employed (U.S. Pat. No. 5,981,840, and PCT patent publication WO98/32326; the contents of which are hereby incorporated by reference). Briefly, immature embryos were isolated from maize and the embryos contacted with a suspension of  Agrobacterium  under conditions whereby the bacteria were capable of transferring the promoter sequence of the invention to at least one cell of at least one of the immature embryos (step 1: the infection step). In this step the immature embryos were immersed in an  Agrobacterium  suspension for the initiation of inoculation. The embryos were co-cultured for a time with the  Agrobacterium  (step 2: the co-cultivation step). The immature embryos were cultured on solid medium following the infection step. Following the co-cultivation period and an optional “resting” step was performed. In this resting step, the embryos were incubated in the presence of at least one antibiotic known to inhibit the growth of  Agrobacterium  without the addition of a selective agent for plant transformants (step 3: resting step). The immature embryos were cultured on solid medium with antibiotic, but without a selecting agent, for elimination of  Agrobacterium  and for a resting phase for the infected cells. Next, inoculated embryos were cultured on medium containing a selective agent and growing transformed callus was recovered (step 4: the selection step). The immature embryos were cultured on solid medium with a selective agent resulting in the selective growth of transformed cells. The callus was then regenerated into plants (step 5: the regeneration step), and calli grown on selective medium were cultured on solid medium to regenerate the plants. 
     EXAMPLE 6 
     Expression of MBP-RX002 Fusion in Transgenic Maize Tissue 
     Transgenic events derived from the testing vector were evaluated for expression of MBP-RX002 by Western analysis. Leaf and root material for transgenic maize expressing MBP-RX002 were lyophilized then powdered with 5/32 inch BBs (i.e., birdshot) using a Geno/Grinder 2000 homogenizer at 1700 beats per minute for 30 seconds. 80 μL of grinding buffer (1×PBS+0.1% Tween-20+1% 2-mercaptoethanol containing Roche cOmplete protease inhibitor (Roche Applied Science, Indianapolis, Ind., USA; Catalog No. 04693124001; at one tablet per 7 mL) was added to each sample. Pulverization was repeated for an additional 30 seconds, then the samples were sonicated for 5 minutes at room temperature in a VWR 75D sonicator. After centrifugation at 21,000 g/4° C. for 15 minutes, supernatants were collected. Protein concentrations were determined from the supernatants using Thermo Scientific Coomassie Plus Kit (23236) and a SpectraMAX 190 spectrophotometer. Samples were normalized for total protein in 21 μL using grinding buffer as diluent. Seven microliters of 4× LDS dye containing 1% 2-mercaptoethanol was added to each sample prior to heating at 80° C. for 10 minutes. Twenty-five microliters of sample was loaded per lane on a NuPAGE Novex 4-12% Bis-Tris midi gel (Invitrogen WB1402BOX) and electrophoresed at 200 V for 1 hour. Protein was transferred to a nitrocellulose membrane using an Invitrogen iBlot with a transfer stack (IB301001). The membrane was blocked for 30 minutes in 1×PBS+0.1% Tween-20+5% powdered milk w/v (blocking buffer), then incubated overnight at 4° C. with rabbit polyclonal antibody against RX002, diluted in blocking buffer at 1:4000. Membrane was washed 4×5 minutes in PBST (1×PBS+0.1% Tween-20), then incubated for 2 hours with goat anti-rabbit HRP conjugated secondary antibody (Pierce 31460; 10 μg/mL working stock) at a 1:5000 dilution in blocking buffer. The membrane was washed 4×5 minutes in PBST and developed using Thermo Super Signal West Dura Extended Duration Substrate (34076). Visualization of hybridization signal was accomplished using a Fujifilm LAS-4000 imaging system. 
     The results of this analysis are shown in Table 7. Accumulation of MBP-RX002 was detected in 8 of the 10 events sampled for Western analysis in either root or leaf and root tissue. An 118 kD protein band corresponding to the expected size of the MBP-RX002 fusion was observed in both leaf and root or root tissue demonstrating that the fusion can be expressed in planta. In addition to the full length protein, a 75 kD immunoreactive protein band corresponding to the expected size of RX002 was also observed in some events indicating that some proportion of the full-length fusion protein was processed in planta to release RX002. 
     
       
         
           
               
               
               
             
               
                 TABLE 7 
               
               
                   
               
               
                 Event No. 
                 Expression in leaf 
                 Expression in root 
               
               
                   
               
             
            
               
                 Control 
                 − 
                 − 
               
               
                 123434603 
                 − 
                 − 
               
               
                 123434607 
                 +++ 
                 ++++ 
               
               
                 123434610 
                 − 
                 − 
               
               
                 123434613 
                 ++++ 
                 ++++ 
               
               
                 123434614 
                 − 
                 + 
               
               
                 123434615 
                 − 
                 ++ 
               
               
                 123434616 
                 ++++ 
                 ++++ 
               
               
                 123434617 
                 ++++ 
                 ++++ 
               
               
                 123434618 
                 ++++ 
                 ++++ 
               
               
                 123434619 
                 − 
                 +++ 
               
               
                   
               
            
           
         
       
     
     The article “a” and “an” are used herein to refer to one or more than one (i.e., to at least one) of the grammatical object of the article. By way of example, “an element” means one or more element. 
     All publications and patent applications mentioned in the specification are indicative of the level of those skilled in the art to which this invention pertains. All publications and patent applications are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. 
     Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the appended claims.