Patent Publication Number: US-2023149565-A1

Title: Dual aav-myo7a vectors with improved safety for the treatment of ush1b

Description:
RELATED APPLICATIONS 
     This application claims the benefit of the filing date of U.S. Provisional Application No. 63/003,774, filed Apr. 1, 2020, the entire contents of which are incorporated by reference. 
    
    
     NON-FEDERAL SUPPORT 
     This invention was made in whole or in part from funding under grant award number TA-GT-0419-0774-UFL-GH received from the Foundation for Fighting Blindness, and under agreement number AGR00018211, received from Atsena Therapeutics, Inc. 
     BACKGROUND OF THE DISCLOSURE 
     Recombinant AAV has emerged as a useful gene delivery vehicle to treat retinal disease. However, one limitation of AAV is its relatively small DNA packaging capacity-approximately 4.7 kilobases (KB). Thus, standard AAV vector systems are unsuitable for addressing diseases in which large genes are mutated or otherwise dysfunctional, such as Usher syndrome. A solution is needed in order to package large genes into AAV vector systems and safely deliver gene therapy treatment to patients. 
     SUMMARY OF THE DISCLOSURE 
     The disclosure relates generally to the fields of molecular biology and virology, and in particular, to the development of gene delivery vehicles. Disclosed are improved rAAV dual vector and polynucleotide vector systems, and compositions useful in delivering a variety of nucleic acid segments, including those encoding therapeutic proteins, polypeptides, peptides, antisense oligonucleotides, or ribozyme constructs to selected host cells for use in various gene-therapy regimens. Further disclosed are recombinant viral particles, isolated host cells, and pharmaceutical compositions comprising any of these rAAV dual vector and polynucleotide vector systems. Methods are also provided for preparing and using the improved rAAV dual vector systems disclosed herein in a variety of viral-based gene therapies, and in particular, for the treatment and/or amelioration of symptoms of Myosin VII-deficiency, including, without limitation, the treatment of human Usher syndrome type IB. Further provided herein are methods of treatment or amelioration of a disease or condition involving the administration of rAAV dual vector systems that encode the MYO7A protein and result in reduced cytotoxicities than previously available vector systems. In some aspects, provided are methods of administering a vector system, whereby an amount of truncated MYO7A protein and/or associated cytotoxicity is minimized. In some embodiments, the therapeutic polypeptide is not a myosin polypeptide. 
     In various aspects, the methods of treatment and pharmaceutical compositions provided herein are intended for administration to one or both eyes of a subject, e.g., a human or animal subject. In further various aspects, the methods of treatment and pharmaceutical compositions provided herein are intended for administration to one or both ears of a subject, e.g., a human or animal subject. 
     The disclosure provides materials and methods for gene therapy of diseases, such as Usher syndrome. Usher syndrome, including types I (e.g., USH1B), II, and III, is a condition that results in sensory impairment, specifically in the visual, auditory, and vestibular systems. The sensory loss that accompanies Usher syndrome can be present even at birth, and gets progressively worse with age. 
     The most common form of Usher syndrome, USH1B, is a severe autosomal-recessive, deaf-blindness disorder caused by mutations in the MyosinVIIa gene. Patients are born deaf due to insufficient expression of human Myosin VII protein (MYO7A) and/or mutations in the gene causing protein malfunction. Blindness occurs from a progressive retinal degeneration that begins within the first decade of life. MYO7A protein is expressed in photoreceptors and retinal pigment epithelium (RPE), and is involved in opsin transport through photoreceptor cilia and the movement of RPE melanosomes. A study showed that photoreceptors (PRs) may be the initial site of disease, and that defects in an adhesion belt structure that sits around the photoreceptor outer segment in humans may cause the retinal degeneration seen in USH1B patients (Sahly, et al., 2012). The coding region for the MYO7A protein, however, is 6534 or 6648 nucleotides in length (depending on the isoform), making traditional AAV vector systems unsuitable for gene therapy of USH1B. 
     While there are currently no treatments available for this condition, gene therapy offers promise for recovering/maintaining function within the visual, auditory, and vestibular systems. Previously, Allocca et al. (2008) published results suggesting that AAV5 serotype vectors were capable of packaging genomes of up to 8.9 KB in size, and that these vectors expressed full-length proteins when delivered in vivo. In Allocca et al. (2008), the authors expressed full-length MYO7A protein from an AAV5 vector containing the CMV promoter driving hMYO7A. Subsequent studies confirmed that these ‘oversized’ AAV5 vectors did indeed drive full-length protein expression, however the genetic content of each vector capsid was found to be limited only to ˜5 KB of DNA, and not the 8.7 KB originally reported by Allocca et al. (2008) (Lai et al., 2010; Dong et al., 2010; Wu et al., 2010). These vector capsids were shown to contain a “heterogeneous mixture” of truncated vector genomes (e.g., the 5′ end of the gene, the 3′ end of the gene, or a mixture of the two with an internal sequence deletion). Additionally, these oversized/heterogeneous vectors exhibited poor packaging efficiency (for example, resulting in low-vector titers) and low transduction efficiency when compared to matched reporter vectors of standard size (&lt;5 KB) (Wu et al., 2010). 
     Using the ‘heterogeneous’ system as described in Lai et al. (2010), Dong et al. (2010) and Wu et al. (2010), vectors containing portions of the MYO7A transgene were packaged despite the observed poor packaging efficiency, and proof-of-concept results were demonstrated in the shaker-1 mouse model of USH1B. The therapeutic results achieved with the heterogeneous AAV-hMYO7A vectors were comparable to previous gene replacement results using a lentivirus-based hMYO7A vector (Hashimoto et al., 2007). This lentivirus-MYO7A vector is under development by Oxford BioMedica in collaboration with Sanofi-Aventis for a phase I/H clinical trial of USH1B, marketed under the name UshStat® LentiVector®. Lentivirus is regarded as a vector platform that is not well-suited for infecting post-mitotic (for example, non-dividing) cells. Furthermore, although the vector is suitable for transducing RPE, many studies have shown it to be ineffective at transducing adult photoreceptors. Because photoreceptors (PRs) may be the initial site of disease (Sahly, et al., 2012), the exclusive targeting by UshStat® of RPE cells may not bring about a complete or effective therapy, although this remains to be seen in human clinical trials. 
     Because of the excellent safety profile and encouraging reports of efficacy in the AAV gene therapy trials for LCA2/RPE65, there has been continuing interest in creating an AAV-based system for treating USH1B patients. The inventors have previously characterized AAV dual vector platforms for use in treating USH1B patients, also described herein. The original dual vector systems designed by the inventors (e.g., the “first generation” dual vector systems) have successfully demonstrated that mRNA arising from the system is 100% accurate relative to what would be predicted by correct homologous recombination of the front and back vector pairs, making them useful as gene therapy delivery vector systems. These vectors are described in US Patent Publication Nos. 2019/0153050 and 2014/0256802, each of which is incorporated herein by reference in its entirety. 
     This disclosure is based, at least in part, on the observation that some of the previous dual vector platforms resulted in the production of truncated MYO7A protein that was correlated with production of a truncated fragment of the MYO7A protein within the cell. Specifically, loss of retinal structure/function was observed following injection of a previous, first-generation dual vector hybrid system into mouse retina, which may have been attributable to the gain of function exerted by truncated MYO7A protein containing a portion of the tail domain. Hybrid vector systems contain both recombinogenic and spliceosome-recognition sequences that enable two paths through which the two halves of the polynucleotide vector system can combine in a cell to make a full-length polynucleotide. Hybrid vector systems are thus modular and versatile alternatives to simple overlap and simple trans-splicing dual vector systems. Described herein are modified dual hybrid vector systems that shift (all of, or a portion of) the coding sequence for the MYO7A tail domain from the front-half vector to the back-half vector by altering the split point (e.g., from between exons 23 and 24, to between exons 21 and 22) in order to eliminate the production of a truncated MYO7A protein and any associated cytotoxicity (for example, a gain of function toxicity observed in the retina). Further described herein are modified dual overlap vector systems that shift the coding sequence for the MYO7A tail domain from the front-half vector to the back-half vector by altering the overlapping coding sequence among the two vector halves. 
     Further described herein are codon-modified hybrid and overlap vector systems in which putative stop codons in non-coding sequences are removed. Further described herein are modified overlap vector systems that contain altered and/or reduced lengths of the overlapping coding sequence between the two vectors. Further described herein are modified hybrid vector systems that contain reductions in the lengths of the back half vector. 
     This disclosure is also based, at least in part, on the improvement of a previous, first-generation dual vector overlap system to increase transduction efficiency in the retina. In some embodiments, the disclosed improvements encompass the shortening of 5′ (front) and/or 3′ (back) AAV vectors in the system to increase rAAV particle packaging efficiencies. 
     In some embodiments, the disclosed rAAV vectors comprise a transgene encoding a MYO7A protein, e.g., human MYO7A protein. In some embodiments, the disclosed rAAV vectors comprise transgenes that encode other proteins relevant to Usher syndrome. In some embodiments, the disclosed rAAV vectors comprise transgenes that encode other proteins relevant to other ocular or aural diseases, disorders, or conditions. 
     Accordingly, aspects of the disclosure provide modified dual AAV vector systems that permit expression of full-length proteins, whose coding sequence exceeds the polynucleotide packaging capacity of an individual AAV vector. 
     Thus, in some aspects, provided herein are hybrid dual vector systems. Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide followed by a splice donor (SD) site and an intron, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats an intron and a splice acceptor (SA) site for the intron, wherein the intron sequence in the first and second AAV vectors comprises a polynucleotide sequence that overlaps, and wherein the split point between the first and second AAV vector polynucleotide sequences is between exon 21 and exon 22 of the hMYO7A gene (see  FIGS.  22 D and  22 E ). Provided herein are hybrid polynucleotide systems in which the N-terminal part of the myosin polypeptide does not comprise the single-alpha helix (SAH) domain of the myosin polypeptide (e.g., in which the first AAV vector polynucleotide comprises a partial coding sequence that does not encode the SAH domain of the myosin polypeptide). In some embodiments, the intron sequence that overlaps comprises an alkaline phosphatase intron. Further provided herein are polynucleotide vector systems wherein the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 33 or 34, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 32, 35, or 44. 
     In other aspects, provided herein are overlap dual vector systems. Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a partial coding sequence that encodes a C-terminal part of the myosin polypeptide, wherein the polynucleotide sequence encoding the polypeptide sequence in the first and second AAV vectors comprises a polynucleotide sequence that overlaps, and wherein the C-terminal part of the myosin polypeptide comprises the single-alpha helix (SAH) domain of the myosin polypeptide. Further provided herein are polynucleotide vector systems wherein the first AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the nucleotide sequence of SEQ ID NO: 36, and the second AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the nucleotide sequence of SEQ ID NO: 38. 
     In some aspects, provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a partial coding sequence that encodes a C-terminal part of the myosin polypeptide, wherein the polynucleotide sequence encoding the polypeptide sequence in the first and second AAV vectors comprises a polynucleotide sequence that overlaps, and wherein (i) the first AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 63, 90, or 66, and (ii) the second AAV vector polynucleotide comprises a nucleic acid sequence at least about 80% at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 77 or 80. 
     Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a partial coding sequence that encodes a C-terminal part of the myosin polypeptide, wherein the polynucleotide sequence encoding the polypeptide sequence in the first and second AAV vectors comprises a polynucleotide sequence that overlaps, and wherein (i) the first AAV vector polynucleotide comprises a nucleotide sequence selected from SEQ ID NOs: 63, 90, and 66, and (ii) the second AAV vector polynucleotide comprises a nucleotide sequence selected from SEQ ID NOs: 77 and 80. 
     Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a partial coding sequence that encodes a C-terminal part of the myosin polypeptide, wherein the polynucleotide sequence encoding the polypeptide sequence in the first and second AAV vectors comprises a polynucleotide sequence that overlaps, and wherein (i) the first AAV vector polynucleotide encodes an amino acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the amino acid sequence of SEQ ID NO: 62, 91, or 65, and (ii) the second AAV vector polynucleotide encodes an amino acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the amino acid sequence of SEQ ID NO: 78 or 81. 
     Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a partial coding sequence that encodes a C-terminal part of the myosin polypeptide, wherein the polynucleotide sequence encoding the polypeptide sequence in the first and second AAV vectors comprises a polynucleotide sequence that overlaps, and wherein the polynucleotide sequence that overlaps comprises a nucleotide sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to a sequence selected from any one of SEQ ID NOs: 39 and 52-59. 
     Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a partial coding sequence that encodes a C-terminal part of the myosin polypeptide, wherein the polynucleotide sequence encoding the polypeptide sequence in the first and second AAV vectors comprises a polynucleotide sequence that overlaps, and wherein the polynucleotide sequence that overlaps comprises a sequence encoding any one of SEQ ID NOs: 79 and 82-89. 
     Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide followed by a splice donor site and a first intron, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a second intron and a splice acceptor site for the first intron, wherein the nucleotide sequences of the first and second introns (collectively referred to herein as “the intron sequence”) comprise a polynucleotide sequence that overlaps, and wherein the first and/or second intron sequence comprises a nucleic acid sequence at least about 80% at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 69 or SEQ ID NO: 70. 
     Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide followed by a splice donor site and a first intron, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a second intron and a splice acceptor site for the first intron, wherein the nucleotide sequences of the first and second introns comprise a polynucleotide sequence that overlaps, and wherein the split point between the first and second AAV vector polynucleotide sequences is between two exons of the gene encoding the therapeutic protein. 
     Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide followed by a splice donor site and a first intron, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a second intron and a splice acceptor site for the first intron, wherein the nucleotide sequences of the first and second introns comprise a polynucleotide sequence that overlaps, and wherein (i) the first AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NOs: 31, 33, 34, and 46, and (ii) the second AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NOs: 32, 35, 44, and 47-49. 
     Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide followed by a splice donor site and a first intron, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a second intron and a splice acceptor site for the first intron, wherein the nucleotide sequences of the first and second introns comprise a polynucleotide sequence that overlaps, and wherein (i) the first AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the nucleotide sequence of SEQ ID NO: 33, and (ii) the second AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the nucleotide sequence of SEQ ID NO: 32. 
     Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide followed by a splice donor site and a first intron, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a second intron and a splice acceptor site for the first intron, wherein the nucleotide sequences of the first and second introns comprise a polynucleotide sequence that overlaps, and wherein (i) the first AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the nucleotide sequence of SEQ ID NO: 34, and (ii) the second AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the nucleotide sequence of SEQ ID NO: 35. 
     Provided herein are polynucleotide vector systems comprising: i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide followed by a splice donor site and a first intron, and ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a second intron and a splice acceptor site for the first intron, wherein the nucleotide sequences of the first and second introns comprise a polynucleotide sequence that overlaps, and wherein (i) the first AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the nucleotide sequence of SEQ ID NO: 34, and (ii) the second AAV vector polynucleotide comprises a nucleic acid sequence at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the nucleotide sequence of SEQ ID NO: 44. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       For promoting an understanding of the principles of the disclosure, reference will now be made to the embodiments, or examples, illustrated in the drawings and specific language will be used to describe the same. It will nevertheless be understood that no limitation of the scope of the disclosure is thereby intended. Any alterations and further modifications in the described embodiments, and any further applications of the principles of the disclosure as described herein are contemplated as would normally occur to one of ordinary skill in the art to which the disclosure relates. 
         FIG.  1    shows the formation of complete gene cassette from dual AAV vectors via homologous recombination. 
         FIG.  2    shows a schematic of the two vector components that make up the Overlap Dual Vector System in accordance with one aspect of the disclosure. 
         FIG.  3    shows a schematic of the two vector components that make up an exemplary Hybrid Dual Vector System containing a native intron. Native hMYO7A intron 23 in shown in light shading; splice donor and splice acceptor sequences are shown in darker shading and indicated with a (•). 
         FIG.  4    shows the schematic of the two vector components that make up an exemplary Hybrid Dual Vector System containing a synthetic intron. This is an exemplary standard trans splicing dual vector system, with the “intron” referring to the synthetic alkaline phosphatase splice donor and acceptor sites. Synthetic alkaline phosphatase (AP) intron is shown in light shading; AP splice donor and splice acceptor sequences are shown in darker shading and indicated with a (•). 
         FIG.  5    shows an immunoblot to detect the presence of MYO7A in infected or transfected HEK293 cells. Heterogeneous vectors are compared to all three dual vector systems. Dual vectors were packaged either in AAV2 or in AAV2 (triple mutant) capsids. The triple mutant contains three tyrosine-to-phenylalanine mutations on the capsid surface. For all three dual vector systems, infections were performed with either a) the front-half (N-terminal) and back-half (C-terminal) vectors; or b) the front-half vectors alone (to confirm the presence or absence of a truncated protein product expressed from the promoter-containing N-terminal vectors). 
         FIG.  6 A  and  FIG.  6 B  show immunoblot to detect the presence of MYO7A in HEK293 cells infected with an exemplary Overlap Dual Vector System. Results are presented as a time course from 3-7 days post infection (lanes 3-7) and are compared to cells transfected with MYO7A plasmid (lane 1) and uninfected control (lane 2). An area of interest in  FIG.  6 A  is magnified and presented at higher contrast in  FIG.  6 B . Starting at 3 days post-infection, full-length human MYO7A protein was visible, with peak expression occurring around day 5. 
         FIG.  7 A  and  FIG.  7 B  show retinas from untreated mice and mice treated subretinally with an exemplary Overlap Dual Vector System. Immunohistochemistry (IHC) was performed using an antibody directed against MYO7A. MYO7A stains and nuclear (DAPI) stains are indicated. 
         FIGS.  8 A- 8 D  show differences in RPE melanosome localization in wild type vs. shaker-1 mice. In wild type mice, RPE melanosome apically migrate towards photoreceptor outer segments ( FIG.  8 A ) whereas this phenomenon fails to occur in mice lacking MYO7A (shaker-1), as seen in ( FIG.  8 B ). To the right is a high magnification image of single RPE cells from either a wild type ( FIG.  8 C ) or shaker-1 ( FIG.  8 D ) mouse showing this phenomenon up close. 
         FIGS.  9 A- 9 C  show that apical migration of RPE melanosomes is restored in shaker-1 mice injected with an exemplary Overlap Dual Vector System. Electron microscopy reveals that melanosomes of untreated shaker-1 mice do not apically migrate ( FIG.  9 A ). In shaker-1 mice injected with exemplary overlap vectors (packaged in AAV2), RPE melanosomes migrate apically towards photoreceptors, which can be seen here in both low- and high-magnification images ( FIGS.  9 B and  9 C ). 
         FIGS.  10 A- 10 F  illustrate the expression of MYO7A from single AAV2 and AAV5 vectors in cultured cells.  FIG.  10 A  is a diagram of the viral vector encoding human MYO7A cDNA.  FIG.  10 B  is a western blot of WT eyecup (lane 1), primary RPE cultures derived from MYO7A-null mice and infected with AAV2-MYO7A (lane 2) or AAV5-MYO7A (lane 3), or not infected (lane 4), and primary RPE cultures derived from MYO7A +/−  mice (lane 5). All lanes were immunolabeled with antibodies against actin (as a loading indicator of relative protein loading) and MYO7A.  FIGS.  10 C- 10 F  are immunofluorescence images of primary RPE cell cultures. Cells derived from MYO7A-null mice that were not infected ( FIG.  10 C ), from MYO7A +/−  mice ( FIG.  10 D ), or from MYO7A-null mice infected with either 1×AAV2-MYO7A ( FIG.  10 E ) or 1×AAV5-MYO7A ( FIG.  10 F ). Scale=10 μm. 
         FIGS.  11 A- 11 M  show the expression of MYO7A from AAV2 and AAV5 dual vectors in vivo.  FIGS.  11 A- 11 E  show EM images of MYO7A immunogold labelling of the connecting cilium and pericilium from rod photoreceptors in a MYO7A-null retina.  FIG.  11 A  is a longitudinal section from an untreated MYO7A-null retina (background label only).  FIG.  11 B  and  FIG.  11 C  are longitudinal sections from MYO7A-null retinas treated with AAV2-MYO7A ( FIG.  11 B ) or AAV5-MYO7A ( FIG.  11 C ). Scale=50 nm.  FIG.  11 D  and  FIG.  11 E  are transverse sections of connecting cilia from rod photoreceptors in MYO7A-null retinas treated with AAV2-MYO7A ( FIG.  11 D ) or AAV5-MYO7A ( FIG.  11 E ). Scale=50 nm.  FIG.  11 F  and  FIG.  11 G  show EM images of RPE cells from MYO7A-null retinas treated with AAV2-MYO7A ( FIG.  11 F ) or AAV5-MYO7A ( FIG.  11 G ). Scale=500 nm. Areas indicated by rectangles are enlarged in  FIG.  11 F- 1    and  FIG.  11 G- 1    to show MYO7A immunogold labeling (indicated by circles). Scale=50 nm.  FIG.  11 H  and  FIG.  11 I  show EM image of a longitudinal section of the connecting cilium and pericilium from a rod ( FIG.  11 H ) and a cone ( FIG.  11 I ) photoreceptor in a MYO7A-null retina, treated with AAV2-MYO7A. The section was double-labeled with MYO7A (12-nm gold) and rod opsin (15-nm gold) antibodies. Rod outer segments were labeled with the opsin antibody, while cones were identified by lack of rod opsin labeling in their outer segments. The sections show just the base of the outer segments. Nearly all the label in the connecting cilium is MYO7A, even in the rod. Scale=50 nm.  FIGS.  11 J- 11 M  are bar graphs indicating MYO7A immunogold particle density in the rod photoreceptor cilium and pericilium ( FIG.  11 J  and  FIG.  11 L ) and in the RPE ( FIG.  11 K  and  FIG.  11 M ), following treatment with AAV2-MYO7A ( FIG.  11 J  and  FIG.  11 K ) or AAV5-MYO7A ( FIG.  11 L  and  FIG.  11 M ) at different concentrations. n=3 animals per condition. Bars indicate SEM. 
         FIGS.  12 A- 12 F  show correction of melanosome localization, following subretinal injections with AAV2-MYO7A or AAV5-MYO7A. Light micrographs showing the presence of melanosomes in the apical processes of the RPE in a WT retina ( FIG.  12 A ) and retinas injected with AAV2-MYO7A ( FIG.  12 B ) or AAV5-MYO7A ( FIG.  12 C ). Further away from the injection site ( FIG.  12 D ), melanosomes are present in the apical processes of some RPE cells, but not in others (arrows indicate apical melanosomes; white lines indicate regions where melanosomes are absent from the apical processes).  FIG.  12 E  illustrates a region distant from injection site, where all RPE cells lacked melanosomes in their apical processes. Brackets on left side indicate RPE apical processes. Scale=25 μm.  FIG.  12 F  is a diagram of an eyecup, indicating the relative locations of the images shown in  FIGS.  12 A- 12 E . ONH indicates the optic nerve head. 
         FIG.  13    shows the correction of abnormal levels of opsin in the connecting cilium and pericilium of rod photoreceptors, following subretinal injections with AAV2-MYO7A or AAV5-MYO7A. The bar graph shows opsin immunogold gold particle density along the length of the connecting cilium. Ultrathin sections of retinas from MYO7A-null and WT mice were stained with rod opsin antibody. The MYO7A-null retinas had been untreated, or treated with either 1× or 1:100 AAV2-MYO7A or AAV5-MYO7A. n=3 animals per condition. Bars indicate SEM. 
         FIGS.  14 A- 14 G  show the expression of MYO7A from the overlapping AAV2-MYO7A dual vectors.  FIG.  14 A- 1    and  FIG.  14 A- 2    illustrate a diagram of the overlapping AAV2-MYO7A dual vectors. The overlapping region contains 1365 bases.  FIG.  14 B  is a Western blot of proteins from primary RPE cultures derived from MYO7A-null mice that were either not infected (lane 1), or infected with AAV2-MYO7A (overlap dual) (lane 2); primary RPE cultures derived from MYO7A +/−  mice (lane 3); WT eyecup (lane 4); HEK293 cells transfected with pTR-smCBA-MYO7A (lane 5). All lanes were immunolabeled with anti-MYO7A and anti-actin.  FIGS.  14 C- 14 F  show immunofluorescence of cultured RPE cells transduced with AAV2-MYO7A (overlap dual).  FIGS.  14 C- 14 E  show primary RPE cultures derived from MYO7A-null mice and ARPE19 ( FIG.  14 F ) cells. Scale=10 μm.  FIG.  14 G  is a bar graph indicating the distribution of MYO7A immunogold particle density among RPE cells from retinas of MYO7A-null mice, injected with AAV2-MYO7A(dual). n=3 animals. 
         FIGS.  15 A- 15 G  illustrate correction of mutant phenotypes, following subretinal injections with AAV2-MYO7A (overlap dual).  FIG.  15 A  shows light microscopy of a semithin section from a treated MYO7A-null mouse retina. The region shown is near the injection site. Arrows indicate melanosomes in the apical processes. White lines indicate cells that still show the MYO7A-null phenotype, with an absence of melanosomes in the apical processes. Scale=50 μm.  FIG.  15 B  is a low-magnification immunoEM image of RPE from a retina treated with AAV2-MYO7A (overlap dual). As in  FIG.  15 A , the white line indicated a region that still showed the MYO7A-null phenotype. Rectangle ‘c’, includes melanosomes in the apical region, indicating a corrected RPE cell. Scale=500 nm.  FIGS.  15 C- 15 E  show higher-magnification images of regions outlined by the rectangles shown in  FIG.  15 B . MYO7A immunogold particles are indicated by circles. Scale=50 nm.  FIG.  15 F  is a bar graph illustrating MYO7A immunogold particle density measured in RPE cells from MYO7A-null retinas, WT retinas, or from MYO7A-null retinas treated with AAV2-MYO7A (overlap dual) and determined to be corrected or not corrected by the location of their apical melanosomes. n=3 animals per condition. Bars indicate SEM.  FIG.  15 G  is an immunoEM image of a rod photoreceptor cilium double-labeled with antibodies against MYO7A (small gold particles) and against rod opsin (large gold particles). MYO7A labeling is associated with the connecting cilium and periciliary membrane, indicating expression and correct localization of MYO7A. While this region is devoid of opsin labeling, which is restricted to the disk membranes, it is consistent with the wild type (WT) phenotype, thus indicating correction of the mutant phenotype. Scale=300 nm. 
         FIG.  16    shows the validation of dual AAV vectors for delivery of full-length MYO7A in vivo. Immunoblot showing expression of MYO7A in retinas of wild type (C57BL/6) mice (lane 1), heterozygous shaker-1 +/−  mice (lane 2) and shaker-1 −/−  mice injected with ‘simple overlap’ MYO7A vectors packaged in AAV8(733) vectors. Both N-terminal and C-terminal vectors of the ‘simple overlap’ system were injected at a concentration of 3×10 10  vector genomes/μL. Dual AAV vectors mediated expression of a MYO7A that was identical in size to that found in WT and shaker-1 +/−  mice. β-actin (visualized here in red) was used as a loading control to validate that equal amounts of protein were loaded in each well. 
         FIGS.  17 A- 17 F  show AAV-mediated MYO7A expression in ARPE-19 with fragmented vectors and the simple overlap dual-vector system. Cells were transduced with fragmented vectors: 1×AAV2-MYO7A ( FIG.  17 A ), AAV5-MYO7A ( FIG.  17 B ), 1/100 dilutions thereof ( FIG.  17 C  and  FIG.  17 D ), and the simple overlap dual-vector system: AAV2-MYO7A (dual) ( FIG.  17 F ). Non-transduced cells were used as a control ( FIG.  17 E ); lighter color, MYO7A; darker color, DAPI. Scale=10 μm. 
         FIGS.  18 A- 18 D  show MYO7A expression in the connecting cilium and pericilium of rod photoreceptors from MYO7A-null retinas injected with diluted AAV2-MYO7A ( FIG.  18 A  and  FIG.  18 B ) or AAV5-MYO7A ( FIG.  18 C  and  FIG.  18 D ); ( FIG.  18 A  and  FIG.  18 C ) 1:10, ( FIG.  18 B  and  FIG.  18 D ) 1:100. Scale=200 nm. 
         FIG.  19    shows the structural preservation of injected MYO7A-null retinas. Light microscopy of the photoreceptor layer 3 weeks after injection with 10×AAV5-MYO7A. Scale=15 μm. 
         FIG.  20    shows structural preservation of injected MYO7A-null retinas. Light microscopy of photoreceptor layer 3 months after injection with 1×AAV2-MYO7A. Scale=10 μm. 
         FIGS.  21 A- 21 D  show correction of abnormal levels of opsin in the connecting cilium and pericilium of rod photoreceptors following subretinal injections with AAV2-MYO7A or AAV5-MYO7A. ImmunoEMs from WT retina ( FIG.  21 A ), MYO7A-null retinas treated with 1×AAV2-MYO7A ( FIG.  21 B ) or 1×AAV5-MYO7A ( FIG.  21 C ), and from an untreated MYO7A-null retina ( FIG.  21 D ) labeled with anti-rod opsin and 12-nm gold-conjugated secondary antibody. Scale=200 nm. 
         FIGS.  22 A- 22 E  show a schematic representation of the dual-AAV-vector pairs created for this study.  FIG.  22 A  is a fragmented AAV (fAAV) vector.  FIG.  22 B  shows simple overlap: the 1365-bp shared between the two vectors is shaded gray.  FIG.  22 C  is a trans-splicing vector.  FIG.  22 D  shows an AP hybrid vector: the 270-bp element shared between the two vectors is marked with diagonal gradient shading (⅓ APhead as described by Ghosh et al., 2011).  FIG.  22 E  shows the native intron hybrid vectors utilizing a 250-bp sequence of MYO7A intron 23. 3′ MYO7A is the 3′-portion of MYO7A; 5′ MYO7A is the 5′-portion of MYO7A; AAV is adeno-associated virus; AP is alkaline phosphatase; intron=intron 23 of MYO7A; pA=polyadenylation signal; SA=splice-acceptor site; SD=splice-donor site; and smCBA refers to a (truncated) chimeric cytomegalovirus immediate early/chicken β-actin chimeric promoter. 
         FIGS.  23 A- 23 C  show cells expressing human MYO7A after infection with the simple overlap vectors of the present disclosure.  FIG.  23 A  shows human embryonic kidney (HEK293) cells expressing human MYO7A after infection with simple overlap vectors (MOI of 10,000 for both vectors) packaged in AAV2 (tripleY-F). Equal amounts of protein were separated on 7.5% sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and stained for MYO7A.  FIG.  23 B  shows HEK293 cells infected with AAV2 (tripleY-F) at MOIs of 10,000, 2,000, and 400.  FIG.  23 C  is a time-course assay of MYO7A expressed in HEK293 cells. Cells were harvested 3-7 days after infection. MOI=multiplicity of infection; T=HEK293 cells transfected with full-length MYO7A plasmid; U=untreated HEK293 cells. 
         FIG.  24    shows the comparison of AAV2 and AAV2 (tripleY-F mutant capsid)-based vectors in HEK293 cells. Cells were infected with AP hybrid and simple overlap MYO7A dual vector platforms packaged in AAV2 or AAV2(tripleY-F) at an MOI of 10,000 for each vector. 
         FIGS.  25 A- 25 C  show human MYO7A expressed in HEK293 cells. Cells were infected with AAV2-based vector platforms. For each of the dual vector systems, the corresponding 5′ and 3′ vectors (or the 5′ vector alone) were used for infection. HEK293 cells transfected with MYO7A plasmid were used as a positive control. Cells were infected with the MYO7A dual vector pairs at an MOI of 10,000 for each vector. Protein samples were analyzed on Western blot with an antibody against MYO7A ( FIG.  25 A ). Each dual vector platform&#39;s relative ability to promote reconstitution was compared by quantifying the amount of 5′ vector-mediated truncated protein product in the presence or absence of the respective 3′ vector ( FIG.  25 B ). Full-length MYO7A expression mediated by dual vectors was quantified relative to transfection control ( FIG.  25 C ). 
         FIGS.  26 A- 26 C  show the ability of MYO7A dual vectors to recombine and properly restore coding sequence. The experimental plan is shown in  FIG.  26 A . HEK293 cells were infected with AAV2-based dual vector platforms, RNA was extracted, and gene-specific primers amplified the sequences using PCR. Control digests with BglII (B) and PpuMI (P) revealed the predicted banding pattern shown in  FIGS.  26 B- 26 C . Undigested (U) PCR product is shown as control and a DNA size marker for reference (M). Separately, products were digested with KpnI and AgeI, and then cloned into pUC57 for sequencing of the entire overlap region. Ten clones per vector platform were analyzed. M13 forward- and reverse-primers specific for the subclone vector were used to obtain sense and antisense reads (each 1,000 bp) resulting in 140 bp for which the sense and antisense reads overlapped ( FIG.  26 C ; Table). PCR=polymerase chain reaction. 
         FIGS.  27 A- 27 H  show the dual vector-mediated MYO7A expression in vivo. C57BL/6J mice were injected subretinally with AAV2-based dual vectors containing a C-terminal hemagglutinin (HA) tag. Retinal protein expression was analyzed four weeks post-injection by immunohistochemistry and western blot. Ten-micron frozen retinal cross sections were stained with an antibody for HA and imaged at 10× ( FIG.  27 A ,  FIG.  27 C , and  FIG.  27 E ) and 60× ( FIG.  27 B ,  FIG.  27 D , and  FIG.  27 F ). An untreated C57BL/6J retina was also stained with an antibody against HA ( FIG.  27 G ). Equal amounts of protein were separated on a 4-15% polyacrylamide gel and stained with an HA antibody ( FIG.  27 H ). For comparison, endogenous MYO7A from C57BL/6J retina was probed with an antibody against MYO7A to confirm that HA-tagged MYO7A migrated at the appropriate size. RPE—retinal pigment epithelium, IS—inner segments, OS—outer segments, ONL—outer nuclear layer, INL—inner nuclear layer, GCL—ganglion cell layer, PR—photoreceptors. Nuclear (DAPI) stains are indicated with brackets (}). 
         FIG.  28    is a representative schematic of how the dual vector systems deliver, recombine, and produce full-length transgene. MYO7A cDNA is split into two parts, or “halves,” and each half is delivered via a separate AAV vector. Following co-infection, gene halves recombine via their shared/overlapping sequence to form full-length MYO7A. The recombined transgene can then be transcribed and translated into the desired protein product. 
         FIGS.  29 A- 29 B  show the dual vector-mediated MYO7A expression Myo7a −/−  mice.  FIG.  29 A  shows the resultant protein expression following simple overlap or AP hybrid dual vector injections. Myo7a−/− mice were injected with 5.0×10 8  vector genomes (vg) total (2.5×10 8  vg each) of either the simple overlap or AP hybrid dual vectors. All vector expression was driven by the smCBA promoter. Retinas were collected and analyzed at 6 weeks post-injection.  FIG.  29 B  shows the quantification of the full-length MYO7A expression normalized to vinculin (VCL) for multiple treatment groups. 
         FIG.  30    shows two bar graphs illustrating the scotopic b-wave responses and average ONL thickness by treatment at 6 weeks post-injection, in order to evaluate the safety of dual AAV-MYO7A vectors. All treatments were delivered at 5.0×10 8  vg total (2.5×10 8  vg each). 
         FIGS.  31 A- 31 B  show that only the front half hybrid vectors produced truncated protein.  FIG.  31 A  is a bar graph showing the average MYO7A front half transcript expression at 6 weeks post-injection.  FIG.  31 B  shows the Western Blot results at 6 weeks post-injection, using VCL as a loading control. All vectors were delivered at 5.0×10 8  vg. 
         FIG.  32    shows two bar graphs illustrating the scotopic b-wave responses in C57BL/6J and Myo7a −/−  mice at 6 weeks post-injection. These results show that only the front half hybrid vectors led to a loss of retinal function. All vectors were delivered at 5.0×10 8  vg. 
         FIG.  33    shows two bar graphs illustrating the average MYO7A back half transcript expression and vector genomes at 6 weeks post-injection. These results show that the back half vectors do not produce transcript or truncated protein. All vectors were delivered at 8.0×10 8  vg. 
         FIG.  34    is a diagram showing the split site relocation from the original site, between exons 23 and 24, to a new split site, between exons 21 and 22, in order to prevent a loss of function observed following injection with front half hybrid vectors. The hybrid vector system with the new split site between exons 21 and 22 will be hereinafter referred to as the “second generation” hybrid. 
         FIGS.  35 A- 35 B  show in vitro results after administration of first and second generation hybrid vectors in HEK293 cells.  FIG.  35 A  shows the Western Blot results of the original hybrid, with a split site between exons 23 and 24 (“Ex23/24”), and the second generation hybrid, with a split site between exons 21 and 22 (“Ex21/22”), using VCL as a loading control.  FIG.  35 B  illustrates the normalized full-length MYO7A expression. NI indicates “not injected”. 
         FIGS.  36 A- 36 C  show in vivo results from the subretinal injection of second generation dual hybrid vectors in Myo7a −/−  mice. The injection dose was 5×10 8  vg total.  FIG.  36 A  shows the Western Blot results, using VCL as a loading control.  FIG.  36 B  shows p-values for the comparison of full-length protein expression between exons 21/22 and exons 23/24 in the hybrid vector systems.  FIG.  36 C  displays the normalized MYO7A expression relative to WT. Hybrid vectors were encapsidated and administered in AAV5 and AAV8(Y733F) virions, and the MYO7A expression associated with each serotype was measured and normalized to WT. 
         FIGS.  37 A- 37 B  show schematics of an exemplary second generation MYO7A polynucleotide vector systems of the disclosure.  FIG.  37 A  shows the second generation hybrid vector system, with a split site between exons 21 and 22. AP sequences act as a polyA and/or splicing signal.  FIG.  37 B  shows the overlap vectors with the potential in-frame stop codons from the 3′ end (downstream of the MYO7A N-terminal fragment) in the front hybrid vector plasmid removed. 
         FIG.  38    is a bar graph showing the results from in vivo hybrid AAV-MYO7A injections. The second generation Ex21/22 hybrid vectors express equivalent amounts of full-length MYO7A compared to the first generation Ex23/24 hybrid vectors, but do not cause the production of a truncated protein fragment observed in the first generation Ex23/24 hybrid vectors. 
         FIGS.  39 A- 39 B  show in vitro results of expression of a truncated MYO7A protein in HEK293 cells.  FIG.  39 A  shows the Western blot results of the original hybrid (exons 23/24) front, second generation hybrid (exons 21/22) front, and the hybrid CMv1 (exons 21/22) front. As used herein, “CMv1” (or “COv2”) refers to the human codon-modified version 1 hybrid vector.  FIG.  39 B  is a bar graph showing the truncated MYO7A expression normalized to vinculin. 
         FIG.  40    shows the western blot results for the original hybrid front vector, second generation ex21/22 hybrid front vector, CMv1 ex21/22 hybrid front vector, and the CMv2 ex21/22 hybrid front vector. As used herein, “CMv2” (may be referred to herein as “COv2”) refers to the human codon-modified version 2 hybrid vector. 
         FIG.  41    shows the nonhuman primate data for the expression of AAV-mediated MYO7A transcript in macaque retina and tolerability of dual AAV5-MYO7A vectors in subretinally injected macaque retinas. Vectors were delivered at titers of 4×10 8  vg each (8×10 8  vg total). 
         FIG.  42    shows the procedure for removal of the stop codons from the second generation hybrid front-half vector (“Myo7a NT-Ex21”) to create the CMv1 hybrid front-half vector. The second generation hybrid front-half vector (relevant fragment of SEQ ID NO: 31 shown as SEQ ID NO: 40) contained three stop codons within the AP intron (potential stop codons shown in red). The restriction enzymes Bsu36I and NheI were used to excise the region containing the stop codons. Gibson primer sets (SEQ ID NO: 42, SEQ ID NO: 43) were then used to replace the excised sequence. The new sequence had all three of the potential in-frame stop codons removed (are indicated with asterisks (*)) (relevant fragment of SEQ ID NO: 33 shown as SEQ ID NO: 41). In addition to modifying the potential stop codons, two restriction sites, Hpal and Mfel, were added to the new sequence to simplify the screening of the resultant clones. 
         FIG.  43    shows the changes made in the second generation overlap front-half vector to create the CMv1 overlap front-half vector. 
         FIG.  44    is a schematic that shows the dual AAV vector approach for the delivery of MYO7A gene therapy. The MYO7A cDNA is split into two halves and each half is delivered via a separate AAV vector, delivered in separate AAV particles that are co-injected. The gene halves recombine in each cell to form full-length MYO7A. 
         FIGS.  45 A- 45 C  show two different dual AAV vector platforms that drive full-length MYO7A expression.  FIG.  45 A  contains schematics depicting the overlap and hybrid dual AAV vector platforms.  FIGS.  45 B and  45 C  show the amount of MYO7A produced from the original hybrid and overlap vectors following encapsidation in AAV5 or AAV8(Y733F) virions. VCL marker was used as a control. 
         FIG.  46    shows improved (third generation) overlap dual vectors that show increased packaging efficiency. 
         FIG.  47    shows length and location of overlap. Shorter overlap lengths result in less shared sequence between front and back half vectors. 
         FIGS.  48 A and  48 B  show overlap vectors compared using the Protein Simple Jess quantification system through use of a capillary-based Western blot tool.  FIG.  48 A  shows the expression of MYO7A and truncated MYO7A from various overlap vectors.  FIG.  48 B  shows a table describing the overlap length and the respective ITR-ITR length (bp). 
         FIGS.  49 A- 49 B  show overlap panel quantification. Overlap vectors containing 687 or 945 bp of overlapping MYO7A sequence produce as much or more full length MYO7A as original hybrid vectors.  FIG.  49 A  shows MYO7A expression normalized to VCL relative to the original hybrid vectors.  FIG.  49 B  shows the degree of expression of truncated MYO7A protein normalized to VCL relative to the original hybrid vectors. 
         FIGS.  50 A- 50 D  show improved hybrid dual vectors that provide reduced cytotoxicity and greater safety.  FIG.  50 A  shows Hybrid-V2 with altered ‘split point’ such that no neck/tail domain is encoded by the front half vector.  FIG.  50 B  shows a schematic of the hybrid vectors. Four potential in-frame stop codons were modified in the Hybrid-V2 front half vector sequence. All codon modifications were made on the Hybrid-V2 MIN background.  FIG.  50 C  shows a table describing the modifications made to each vector, and the respective ITR to ITR (ITR-ITR) length (in bp).  FIG.  50 D  shows a comparison of production of full-length MYO7A and truncated MYO7A fragment against the original hybrid vectors. 
         FIGS.  51 A- 51 E  show improved hybrid dual vectors for safety.  FIG.  51 A  shows Hybrid-V2 back MIN, Hybrid-CMv2 back MIN, and an exemplary vector representing Hybrid-V2 back MIN HA and Hybrid-CMv2 back MIN HA. In the Hybrid back MIN vectors, an ‘unneeded legacy’ sequence was removed from the back half vector to ensure the vector size did not exceed packaging capacity.  FIG.  51 B  shows a table describing the codon modifications made to each vector, and the respective ITR-ITR length (bp).  FIGS.  51 C-E  show that reducing the size of back half vectors leads to increased expression of full length MYO7A from hybrid vectors. 
     
    
    
     DETAILED DESCRIPTION OF THE DISCLOSURE 
     Illustrative embodiments of the disclosure are described below. The disclosure provides compositions and methods for genetic therapy of diseases and conditions, such as Usher syndrome 1B (USH1B). Aspects of the disclosure concern AAV-based dual vector systems that allow for expression of full-length proteins whose coding sequence exceeds the polynucleotide packaging capacity of individual AAV vectors. Aspects of this disclosure provide AAV-based dual vector systems for expression in the retina of the eyes of the subject, or the hair cells of the inner ear of a subject. Accordingly, provided herein are methods for treatment of ocular and aural symptoms associated with USH1B, as well as other diseases and disorders. The disclosure provides nucleic acid vectors of an overlap vector system and nucleic acid vectors of a hybrid vector system. 
     Multiple distinct, AAV-based, dual vector systems have been created and disclosed herein for use in gene replacement therapies, including, for example, in the treatment of USH1B in human patients. In particular embodiments, a vector system of the disclosure employs two discrete AAV vectors that each packages a maximal-size DNA molecule (for example, ˜4.5 to 4.8 Kb). The two vectors are co-administered to selected recipient cells to reconstitute the full-length, biologically-active, MYO7A polypeptide. In these constructs, a portion of overlapping nucleic acid sequence is common to each of the vector genomes (see  FIG.  1   ). When co-delivered to suitable cells ( FIG.  44   ), the overlapping sequence region facilitates the proper concatamerization of the two partial gene cassettes. These gene cassettes then undergo homologous recombination to produce a full-length gene cassette within the cells (see  FIG.  1   ). Exemplary shared components of exemplary embodiments of the dual vector systems include the use of AAV inverted terminal repeats (ITR), the small (truncated) version of the chimeric CMV/chicken β-actin promoter (smCBA), human MYO7A (hMYO7A) cDNA sequence and the SV40 polyadenylation (pA) signal. 
     In some embodiments, the polynucleotide vector and vector systems provided herein do not comprise any of the nucleotide sequences of SEQ ID NOs: 1-4. In exemplary embodiments, the overlap vectors of the disclosure do not comprise any of SEQ ID NOs: 1 and 2. In exemplary embodiments, the hybrid vectors of the disclosure do not comprise any of the nucleotide sequences of SEQ ID NOs: 3 and 4. In some embodiments, the vectors of the disclosure do not comprise the nucleotide sequence of SEQ ID NO: 67 or NO: 71. 
     Overlap Vector System 
     In some aspects, overlap dual AAV vector systems are provided. In some embodiments, the overlap vector systems of the disclosure do not produce a truncated MYO7A protein fragment following administration to a mouse or a subject. 
     In one aspect of the disclosure, an overlap vector system of the disclosure includes: 
     i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end (5′ and 3′ end) of the polynucleotide, and between the inverted terminal repeats a suitable promoter followed by (for example, 3′ to the promoter) a partial coding sequence that encodes an N-terminal part of a selected full-length polypeptide, and 
     ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end (for example, the 5′- and 3′-ends) of the polynucleotide, and between the inverted terminal repeats a partial coding sequence that encodes a C-terminal portion of the selected full-length polypeptide, and optionally followed by a polyadenylation (pA) sequence. The coding sequences in the first and second vectors when combined encode the selected full-length polypeptide, or a functional fragment or variant thereof. The polypeptide encoding sequence in the first and second AAV vectors comprises sequence that overlaps. 
     In some embodiments of the provided overlap vector systems, the selected full-length polypeptide is a myosin polypeptide. In some embodiments, the myosin polypeptide is human myosin VII A (hMYO7A). In some embodiments, the myosin polypeptide is human myosin VII B (hMYO7B). In some embodiments, the myosin polypeptide is myosin 7 (VII) isoform II. Isoform II (2) of hMYO7A (NM_001127180) encodes a 2175-amino acid protein (250.2 kDa) and lacks an in-frame segment in the coding region (a portion of exon 35), relative to isoform I (see Chen et al., 1996; Weil et al., 1996). In some embodiments, the myosin polypeptide is another myosin isoform or a functional fragment thereof. In particular embodiments, full-length myosin 7A or isoform II is encoded in the provided vector systems. The peptide sequence of isoform II is set forth as SEQ ID NO: 8. 
     In some embodiments, the selected full-length polypeptide is selected from ABCA4 (Stargardt disease), CEP290 (LCA10), EYS (Retinitis Pigmentosa), RP1 (Retinitis Pigmentosa), ALMS1 (Alstrom syndrome), CDH23 (Usher syndrome 1D), PCDH15 (Usher syndrome 1F), and USHERIN (USH2A) Usher syndrome 2A). In some embodiments, the selected full-length polypeptide is selected from DMD (Duchenne muscular dystrophy), CFTR (Cystic fibrosis), GDE (Glycogen storage disease III), DYSF (dysferlinopathies), OTOF (neurosensory nonsyndromic recessive deafness) and F8 (Hemophilia A). The diseases and disorders associated with each of these genes are provided in parentheses. In some embodiments, the selected full-length polypeptide is encoded by a gene of about 6 Kb to about 9 Kb in length. In some embodiments, the selected full-length polypeptide is encoded by a gene of about 7 Kb to about 8 Kb in length. 
     The inventors have also discovered that hMYO7A overlapping regions, e.g., SEQ ID NOs: 39 and 53-59, may be used as the polynucleotide sequence that overlaps in additional overlap dual vectors expressing large genes (other than MYO7A). Accordingly, in some embodiments, overlap dual vectors expressing portions (or halves) of a large gene selected from ABCA4, CEP290, EYS, RP1, ALMS1, CDH23, PCDH15, USH1C, USH1G, USH2A, DNFB31, DMD, CFTR, GDE, DYSF, F8, and DFNB2, contain an overlap region that comprises a part of the hMYO7A gene in the polynucleotide sequence that overlaps. These overlap vectors express a large gene other than MYO7A and that comprises a nucleotide sequence having at least 80%, 85%, 90%, 95%, 98%, or 99% identity to any one of SEQ ID NOs: 39 and 52-59. Such overlap vectors may comprise an overlapping region that contains the nucleotide sequence of any one of SEQ ID NOs: 39 and 53-59, e.g., SEQ ID NO: 56 or 57. 
     In some embodiments, the selected full-length polypeptide is expressed in one or more photoreceptor cells. In some embodiments, the selected full-length polypeptide is expressed in one or more cells that do not comprise photoreceptor cells. In some embodiments, the selected full-length polypeptide is expressed in one or more hair cells, e.g., hair cells of the auditory system or the vestibular system. 
     In some embodiments, the C-terminal part of the selected full-length polypeptide (e.g., the myosin polypeptide) comprises the single-alpha helix (SAH) domain of the selected full-length polypeptide. 
     In some embodiments, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 1, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 2, or a functional fragment and/or variant thereof. 
     In some embodiments, the first generation overlap vector (for example, the AAV vector polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1, or a functional fragment and/or variant thereof, and/or the second AAV vector polynucleotide comprising the nucleotide sequence of SEQ ID NO: 2, or a functional fragment and/or variant thereof) contains nucleotides 1 through 3644 of MYO7A cDNA from the ATG in the 5′ vector and/or nucleotides 2279 through 6534 in the 3′ vector. In some embodiments, the fragments are amplified with P1 and P3 by polymerase chain reaction (PCR) and cloned into the 5′ vector via NotI and NheI and the 3′ vector with P3 (AflII) and P4 (KpnI), respectively. The resulting two vector plasmids share 1365 bp of overlapping MYO7A sequence ( FIG.  2   ), and the overlap between the sequences ends at the split point between exons 23 and 24. 
     In some embodiments, a portion of the coding sequence present at the 3′-end of the coding sequence of the first generation overlap vector is identical or substantially identical with a portion of the coding sequence present at the 5′-end of the coding sequence of the first generation overlap vector. In particular embodiments, the sequence overlap between the first and second AAV (first generation) overlap vectors of the disclosure is between about 500 and about 3,000 nucleotides; between about 1,000 and about 2,000 nucleotides; between about 1,200 and about 1,800 nucleotides; or between about 1,300 and about 1,400 nucleotides. 
     In particular embodiments, the sequence overlap between the first and second AAV overlap vectors of the disclosure is 1284 bp, 1027 bp, 1026 bp, 945 bp, 687 bp, 361 bp, 279 bp, or 20 bp in length. In particular embodiments, the sequence overlap between the first and second AAV overlap vectors of the disclosure has a length that is within 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides different from 1284 bp, 1027 bp, 1026 bp, 945 bp, 687 bp, 361 bp, 279 bp, or 20 bp. In some embodiments, the sequence overlap is 945 bp, 687 bp, or 361 bp. 
     In particular embodiments, the sequence overlap of the first generation overlap vector system is about 1,350 nucleotides. In an exemplary embodiment, the sequence overlap of the first generation overlap vector system is 1,365 nucleotides. In particular embodiments, the polynucleotide sequence that overlaps comprises SEQ ID NO: 45. In particular embodiments, the polypeptide encoded is wild type or functional human myosin VIIa (hMYO7A). Amino acid sequences of wild type and functional hMYO7A polypeptides, and polynucleotides encoding them, are known in the art (see, e.g., GenBank accession numbers NP_000251 and U39226.1). In particular embodiments, a hMYO7A polypeptide comprises the amino acid sequence shown in SEQ ID NO: 6 or SEQ ID NO: 8, or a functional fragment or a variant thereof. In particular embodiments, the hMYO7A polypeptide is encoded by the nucleotide sequence shown in SEQ ID NO: 5 or SEQ ID NO: 7. 
     Codon-Modified Overlap Vectors 
     In some embodiments of the disclosed overlap vector systems, a codon-modified overlap vector is provided. In some embodiments, the first generation overlap front-half vector (“AAV-smCBA-hMYO7A-NT”) is shortened. All coding sequences corresponding to the tail domain of MYO7A was removed from the front half vector, thus reducing the size of the overlap region to 361 bp (SEQ ID NO: 39). (This vector does not generate a truncated MYO7A fragment containing a tail, or SAH, domain.) The vector was also altered so that all potential (or putative) stop codons were removed. (See  FIG.  37 B .) The resultant vector is the CMv1 overlap front-half vector (“AAV-smCBA-hMYO7A-noDimNT-CMv1”). Accordingly, the overlap vector system of the disclosure may comprise a CMv1 overlap vector system. 
     In some embodiments, an overlap vector having an altered (e.g., a shortened) overlapping coding sequence is provided. In such embodiments, an overlap vector containing an overlap sequence in the MYO7A gene or another gene that is less than 1365 bp in length is provided. In these systems, the length of the overlapping sequence is reduced to a certain point, therefore ensuring neither vector genome is pushing the packaging capacity of AAV capsid (4.7-4.9 kb), leads to increased expression of full length MYO7A. If the overlap length is too small (&lt;361 bp), full length MYO7A expression is reduced, and truncated protein appears. Overlap vectors containing 687 or 945 bp of overlapping MYO7A sequence produce as much or more full-length MYO7A as original hybrid vectors. (See  FIGS.  48 A,  48 B,  49 A , and  49 B.) Such vectors may be referred to herein as “V3” or 3 rd  generation overlap vectors. In exemplary embodiments, overlap vectors containing 687 or 945 bp of overlapping MYO7A sequence are exemplified. 
     Accordingly, provided herein are polynucleotide vector systems wherein the polynucleotide sequence that overlaps comprises a nucleotide sequence selected from any one of SEQ ID NOs: 39 and 52-59. In some embodiments, the polynucleotide sequence that overlaps comprises a nucleotide sequence selected from any one of SEQ ID NOs: 39, 56, and 57. In exemplary embodiments, the polynucleotide sequence that overlaps comprises the sequence of SEQ ID NO: 56 or 57. In some embodiments, the length between the inverted terminal repeats at each end of the first AAV vector polynucleotide is about 4615 nucleotides (nt) or fewer. In some embodiments, the length between the inverted terminal repeats at each end of the second AAV vector polynucleotide is about 4800 nt or fewer. In some embodiments, the length between the inverted terminal repeats at each end of the second AAV vector polynucleotide is about 4560 nt. 
     Thus, in some embodiments, the polynucleotide vector system of the disclosure is a CMv1 overlap system. In some embodiments, the vector system is an overlap V2 (2 nd  generation) system. In some embodiments, the vector system is a V3 overlap (3 rd  generation) system. Any of the disclosed front-half overlap vectors may be combined with any of the disclosed back-half overlap vectors in the compositions of the disclosure. The resulting third generation overlap front half vector (“AAV-smCBA-hMYO7A-NTlong-v3”) is set forth as SEQ ID NO: 50. The resulting third generation overlap back half vector, inclusive of an HA tag, is set forth as SEQ ID NO: 51. 
     Accordingly, in some aspects, provided herein are polynucleotide vector systems comprising: 
     i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide, and 
     ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a partial coding sequence that encodes a C-terminal part of the myosin polypeptide, 
     wherein the polynucleotide sequence encoding the polypeptide sequence in the first and second AAV vectors comprises a polynucleotide sequence that overlaps, and 
     wherein the first AAV vector polynucleotide comprises a nucleotide sequence selected from SEQ ID NOs: 36, 37, and 50; 
     and the second AAV vector polynucleotide comprises a nucleotide sequence selected from SEQ ID NOs: 38 and 51. In exemplary embodiments, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 50, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 51. In some embodiments, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 50, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 38. In some embodiments, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 36, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 38. 
     In some embodiments, the first AAV vector polynucleotide comprises a partial coding sequence that does not encode the single-alpha helix (SAH) domain of the selected full-length polypeptide. In some embodiments, the first AAV vector polynucleotide of the second generation overlap vector comprises the nucleotide sequence of SEQ ID NO: 37, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide of the second generation overlap vector comprises the nucleotide sequence of SEQ ID NO: 38, or a functional fragment and/or variant thereof. 
     In some embodiments, the second generation overlap vector (for example, the AAV vector polynucleotide comprising the nucleotide sequence of SEQ ID NO: 37, or a functional fragment and/or variant thereof, and/or the second AAV vector polynucleotide comprising the nucleotide sequence of SEQ ID NO: 38, or a functional fragment and/or variant thereof) contains nucleotides 1 through 2640 of MYO7A cDNA from the ATG in the 5′ vector and/or nucleotides 2279 through 6534 in the 3′ vector. In some embodiments, the fragments are amplified with P1 and P3 by polymerase chain reaction (PCR) and cloned into the 5′ vector via NotI and NheI and the 3′ vector with P3 (AflII) and P4 (KpnI), respectively. The resulting two vector plasmids share 361 bp of overlapping MYO7A sequence ( FIG.  37   ), and the overlap between the sequences ends at the split point between exons 21 and 22. 
     In some embodiments, the polynucleotide sequence that overlaps does not comprise any portion of exon 23 of the hMYO7A gene. In some embodiments, the polynucleotide sequence that overlaps does not comprise exon 23 in full (e.g., 100% of exon 23). In some embodiments, the polynucleotide sequence that overlaps comprises a portion of exon 17, exon 18 in full, exon 19 in full, exon 20 in full, and a portion of exon 21 of the hMYO7A gene. In some embodiments, the polynucleotide sequence that overlaps comprises a portion of exon 17, a portion of exon 18, a portion of exon 19, a portion of exon 20, and/or a portion of exon 21 of the hMYO7A gene. As used herein, a “portion” may comprise e.g. at least about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%, etc. of the exon and/or intron sequence. 
     In some embodiments, a portion of the coding sequence present at the 3′-end of the coding sequence of the first vector of the second generation overlap vector is identical or substantially identical with a portion of the coding sequence present at the 5′-end of the coding sequence of the second vector of the second generation overlap vector. In particular embodiments, the sequence overlap between the first and second AAV vectors is between about 1 and about 500 nucleotides; between about 100 and about 200 nucleotides; between about 200 and about 300 nucleotides; or between about 300 and about 400 nucleotides. 
     In particular embodiments, the sequence overlap of the second generation overlap vector system is about 350 nucleotides. In an exemplary embodiment, the sequence overlap of the second generation overlap vector system is 361 nucleotides. In particular embodiments, the polynucleotide sequence that overlaps comprises SEQ ID NO: 39. In particular embodiments, the polypeptide encoded is wild type or functional human myosin VIIa (hMYO7A). Amino acid sequences of wild type and functional hMYO7A polypeptides, and polynucleotides encoding them, are known in the art (see, e.g., GenBank accession numbers NP_000251 and U39226.1). In particular embodiments, a hMYO7A polypeptide comprises the amino acid sequence shown in SEQ ID NO: 6 or SEQ ID NO: 8, or a functional fragment or a variant thereof. In particular embodiments, the hMYO7A polypeptide is encoded by the nucleotide sequence shown in SEQ ID NO: 5 or SEQ ID NO: 7. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 5 is a nucleotide sequence encoding a human myosin VIIa polypeptide 
                   
               
               
                 (protein coding sequence is nucleotides 273-6920); 
               
               
                 GCTCTGGGCAGGAGAGAGAGTGAGAGACAAGAGACACACACAGAGAGACGGCG 
               
               
                 AGGAAGGGAAAGACCCAGAGGGACGCCTAGAACGAGACTTGGAGCCAGACAGA 
               
               
                 GGAAGAGGGGACGTGTGTTTGCAGACTGGCTGGGCCCGTGACCCAGCTTCCTGAG 
               
               
                 TCCTCCGTGCAGGTGGCAGCTGTACCAGGCTGGCAGGTCACTGAGAGTGGGCAGC 
               
               
                 TGGGCCCCAGAACTGTGCCTGGCCCAGTGGGCAGCAGGAGCTCCTGACTTGGGAC 
               
               
                 CATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAGATTGGGGCAG 
               
               
                 GAGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAGGTCC 
               
               
                 AGGTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGC 
               
               
                 ACATCAAGCCTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCT 
               
               
                 GGGGGACCTCAACGAGGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGAC 
               
               
                 CACCTCATCTACACGTATACGGGCTCCATCCTGGTGGCTGTGAACCCCTACCAGCT 
               
               
                 GCTCTCCATCTACTCGCCAGAGCACATCCGCCAGTATACCAACAAGAAGATTGGG 
               
               
                 GAGATGCCCCCCCACATCTTTGCCATTGCTGACAACTGCTACTTCAACATGAAAC 
               
               
                 GCAACAGCCGAGACCAGTGCTGCATCATCAGTGGGGAATCTGGGGCCGGGAAGA 
               
               
                 CGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCATCAGTGGGCAGCACTC 
               
               
                 GTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGCATTTGGGAAT 
               
               
                 GCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGACATCC 
               
               
                 ACTTCAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGG 
               
               
                 AAAAGTCACGTGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTA 
               
               
                 CTGCATGCTGGAGGGTATGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCA 
               
               
                 GGCCTCTGACTACAACTACTTGGCCATGGGTAACTGCATAACCTGTGAGGGCCGG 
               
               
                 GTGGACAGCCAGGAGTACGCCAACATCCGCTCCGCCATGAAGGTGCTCATGTTCA 
               
               
                 CTGACACCGAGAACTGGGAGATCTCGAAGCTCCTGGCTGCCATCCTGCACCTGGG 
               
               
                 CAACCTGCAGTATGAGGCACGCACATTTGAAAACCTGGATGCCTGTGAGGTTCTC 
               
               
                 TTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAGGTGAACCCCCCAGACCT 
               
               
                 GATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGACGGTGTCCACC 
               
               
                 CCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGGGATC 
               
               
                 TACGGGCGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGC 
               
               
                 CTCCCTCCCAGGATGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTT 
               
               
                 GGGTTTGAGAACTTTGCTGTGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCA 
               
               
                 ATGAGCACCTGCAGCAGTTCTTTGTGCGGCACGTGTTCAAGCTGGAGCAGGAGGA 
               
               
                 ATATGACCTGGAGAGCATTGACTGGCTGCACATCGAGTTCACTGACAACCAGGAT 
               
               
                 GCCCTGGACATGATTGCCAACAAGCCCATGAACATCATCTCCCTCATCGATGAGG 
               
               
                 AGAGCAAGTTCCCCAAGGGCACAGACACCACCATGTTACACAAGCTGAACTCCC 
               
               
                 AGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAACCATGAGACCCAGTT 
               
               
                 TGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCTTCCTGGAG 
               
               
                 AAGAACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAGGA 
               
               
                 ACAAGTTCATCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAG 
               
               
                 GAAGCGCTCGCCCACACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATG 
               
               
                 CGCACGCTGGGTGCCTGCCAGCCCTTCTTTGTGCGATGCATCAAGCCCAATGAGT 
               
               
                 TCAAGAAGCCCATGCTGTTCGACCGGCACCTGTGCGTGCGCCAGCTGCGGTACTC 
               
               
                 AGGAATGATGGAGACCATCCGAATCCGCCGAGCTGGCTACCCCATCCGCTACAGC 
               
               
                 TTCGTAGAGTTTGTGGAGCGGTACCGTGTGCTGCTGCCAGGTGTGAAGCCGGCCT 
               
               
                 ACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCATGGCTGAGGCTGTGCTGG 
               
               
                 GCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCTGAAGGACCACC 
               
               
                 ATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGTCATCC 
               
               
                 TCCTTCAGAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAA 
               
               
                 GAACGCTGCCACACTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAA 
               
               
                 CTACGGGCTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGG 
               
               
                 AAGCTGCACCAGCAGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCC 
               
               
                 GCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTC 
               
               
                 ACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCACCAACGCCTCA 
               
               
                 GGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGGAAG 
               
               
                 AGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGC 
               
               
                 AAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTG 
               
               
                 AAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAG 
               
               
                 GGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTC 
               
               
                 CTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTG 
               
               
                 AGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCC 
               
               
                 CTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGT 
               
               
                 TCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACCCGGCGGCCACT 
               
               
                 CAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCCCTGGC 
               
               
                 GGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCAC 
               
               
                 ACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAG 
               
               
                 ACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGC 
               
               
                 GAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTG 
               
               
                 CATTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTG 
               
               
                 CATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACC 
               
               
                 TCCAACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAGCAC 
               
               
                 TCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAA 
               
               
                 GAGCAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCC 
               
               
                 CCTCCGAGAAGTTTGTCAAGTACCTGCGGAACTTCATCCACGGGGGCCCGCCCGG 
               
               
                 CTACGCCCCGTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTCAATGGGACACGG 
               
               
                 ACACAGCCGCCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCAATC 
               
               
                 ATGTTGCCCGTGACATTCATGGATGGGACCACCAAGACCCTGCTGACGGACTCGG 
               
               
                 CAACCACGGCCAAGGAGCTCTGCAACGCGCTGGCCGACAAGATCTCTCTCAAGG 
               
               
                 ACCGGTTCGGGTTCTCCCTCTACATTGCCCTGTTTGACAAGGTGTCCTCCCTGGGC 
               
               
                 AGCGGCAGTGACCACGTCATGGACGCCATCTCCCAGTGCGAGCAGTACGCCAAG 
               
               
                 GAGCAGGGCGCCCAGGAGCGCAACGCCCCCTGGAGGCTCTTCTTCCGCAAAGAG 
               
               
                 GTCTTCACGCCCTGGCACAGCCCCTCCGAGGACAACGTGGCCACCAACCTCATCT 
               
               
                 ACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGAGAAGGAGG 
               
               
                 ACGACCTGGCTGAGCTGGCCTCCCAGCAGTACTTTGTAGACTATGGCTCTGAGAT 
               
               
                 GATCCTGGAGCGCCTCCTGAACCTCGTGCCCACCTACATCCCCGACCGCGAGATC 
               
               
                 ACGCCCCTGAAGACGCTGGAGAAGTGGGCCCAGCTGGCCATCGCCGCCCACAAG 
               
               
                 AAGGGGATTTATGCCCAGAGGAGAACTGATGCCCAGAAGGTCAAAGAGGATGTG 
               
               
                 GTCAGTTATGCCCGCTTCAAGTGGCCCTTGCTCTTCTCCAGGTTTTATGAAGCCTA 
               
               
                 CAAATTCTCAGGCCCCAGTCTCCCCAAGAACGACGTCATCGTGGCCGTCAACTGG 
               
               
                 ACGGGTGTGTACTTTGTGGATGAGCAGGAGCAGGTACTTCTGGAGCTGTCCTTCC 
               
               
                 CAGAGATCATGGCCGTGTCCAGCAGCAGGGAGTGCCGTGTCTGGCTCTCACTGGG 
               
               
                 CTGCTCTGATCTTGGCTGTGCTGCGCCTCACTCAGGCTGGGCAGGACTGACCCCG 
               
               
                 GCGGGGCCCTGTTCTCCGTGTTGGTCCTGCAGGGGAGCGAAAACGACGGCCCCCA 
               
               
                 GCTTCACGCTGGCCACCATCAAGGGGGACGAATACACCTTCACCTCCAGTAATGC 
               
               
                 TGAGGACATTCGTGACCTGGTGGTCACCTTCCTAGAGGGGCTCCGGAAGAGATCT 
               
               
                 AAGTATGTTGTGGCCCTGCAGGATAACCCCAACCCCGCAGGCGAGGAGTCAGGCT 
               
               
                 TCCTCAGCTTTGCCAAGGGAGACCTCATCATCCTGGACCATGACACGGGCGAGCA 
               
               
                 GGTCATGAACTCGGGCTGGGCCAACGGCATCAATGAGAGGACCAAGCAGCGTGG 
               
               
                 GGACTTCCCCACCGACTGTGTGTACGTCATGCCCACTGTCACCATGCCACCGCGG 
               
               
                 GAGATTGTGGCCCTGGTCACCATGACTCCCGATCAGAGGCAGGACGTTGTCCGGC 
               
               
                 TCTTGCAGCTGCGAACGGCGGAGCCCGAGGTGCGTGCCAAGCCCTACACGCTGGA 
               
               
                 GGAGTTTTCCTATGACTACTTCAGGCCCCCACCCAAGCACACGCTGAGCCGTGTC 
               
               
                 ATGGTGTCCAAGGCCCGAGGCAAGGACCGGCTGTGGAGCCACACGCGGGAACCG 
               
               
                 CTCAAGCAGGCGCTGCTCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGCAGGAG 
               
               
                 GCCTGCCTGGCCTTCATTGCTGTGCTCAAGTACATGGGCGACTACCCGTCCAAGA 
               
               
                 GGACACGCTCCGTCAACGAGCTCACCGACCAGATCTTTGAGGGTCCCCTGAAAGC 
               
               
                 CGAGCCCCTGAAGGACGAGGCATATGTGCAGATCCTGAAGCAGCTGACCGACAA 
               
               
                 CCACATCAGGTACAGCGAGGAGCGGGGTTGGGAGCTGCTCTGGCTGTGCACGGG 
               
               
                 CCTTTTCCCACCCAGCAACATCCTCCTGCCCCACGTGCAGCGCTTCCTGCAGTCCC 
               
               
                 GAAAGCACTGCCCACTCGCCATCGACTGCCTGCAACGGCTCCAGAAAGCCCTGAG 
               
               
                 AAACGGGTCCCGGAAGTACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAGCA 
               
               
                 CAAGACCACCCAGATTTTCCACAAGGTCTACTTCCCTGATGACACTGACGAGGCC 
               
               
                 TTCGAAGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCACCA 
               
               
                 GGCTGCTCCTCAAGTCCTCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGACAA 
               
               
                 GGTCATCAGCGTTCCTGAGAATGACTTCTTCTTTGACTTTGTTCGACACTTGACAG 
               
               
                 ACTGGATAAAGAAAGCTCGGCCCATCAAGGACGGAATTGTGCCCTCACTCACCTA 
               
               
                 CCAGGTGTTCTTCATGAAGAAGCTGTGGACCACCACGGTGCCAGGGAAGGATCCC 
               
               
                 ATGGCCGATTCCATCTTCCACTATTACCAGGAGTTGCCCAAGTATCTCCGAGGCTA 
               
               
                 CCACAAGTGCACGCGGGAGGAGGTGCTGCAGCTGGGGGCGCTGATCTACAGGGT 
               
               
                 CAAGTTCGAGGAGGACAAGTCCTACTTCCCCAGCATCCCCAAGCTGCTGCGGGAG 
               
               
                 CTGGTGCCCCAGGACCTTATCCGGCAGGTCTCACCTGATGACTGGAAGCGGTCCA 
               
               
                 TCGTCGCCTACTTCAACAAGCACGCAGGGAAGTCCAAGGAGGAGGCCAAGCTGG 
               
               
                 CCTTCCTGAAGCTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTCGAGGTG 
               
               
                 AAGCAAACTACGGAGCCAAACTTCCCTGAGATCCTCCTAATTGCCATCAACAAGT 
               
               
                 ATGGGGTCAGCCTCATCGATCCCAAAACGAAGGATATCCTCACCACTCATCCCTT 
               
               
                 CACCAAGATCTCCAACTGGAGCAGCGGCAACACCTACTTCCACATCACCATTGGG 
               
               
                 AACTTGGTGCGCGGGAGCAAACTGCTCTGCGAGACGTCACTGGGCTACAAGATG 
               
               
                 GATGACCTCCTGACTTCCTACATTAGCCAGATGCTCACAGCCATGAGCAAACAGC 
               
               
                 GGGGCTCCAGGAGCGGCAAGTGAACAGTCACGGGGAGGTGCTGGTTCCATGCCT 
               
               
                 GCTCTCGAGGCAGCAGTGGGTTCAGGCCCATCAGCTACCCCTGCAGCTGGGGAAG 
               
               
                 ACTTATGCCATCCCGGCAGCGAGGCTGGGCTGGCCAGCCACCACTGACTATACCA 
               
               
                 ACTGGGCCTCTGATGTTCTTCCAGTGAGGCATCTCTCTGGGATGCAGAACTTCCCT 
               
               
                 CCATCCACCCCTCTGGCACCTGGGTTGGTCTAATCCTAGTTTGCTGTGGCCTTCCC 
               
               
                 GGTTGTGAGAGCCTGTGATCCTTAGATGTGTCTCCTGTTTCAGACCAGCCCCACCA 
               
               
                 TGCAACTTCCTTTGACTTTCTGTGTACCACTGGGATAGAGGAATCAAGAGGACAA 
               
               
                 TCTAGCTCTCCATACTTTGAACAACCAAATGTGCATTGAATACTCTGAAACCGAA 
               
               
                 GGGACTGGATCTGCAGGTGGGATGAGGGAGACAGACCACTTTTCTATATTGCAGT 
               
               
                 GTGAATGCTGGGCCCCTGCTCAAGTCTACCCTGATCACCTCAGGGCATAAAGCAT 
               
               
                 GTTTCATTCTCTGAAA 
               
               
                   
               
               
                 SEQ ID NO: 6 is the amino acid sequence of the human myosin VIIa polypeptide 
               
               
                 encoded by nucleotides 273-6920 of SEQ ID NO: 5; 
               
               
                 MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATH 
               
               
                 IKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIY 
               
               
                 SPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQ 
               
               
                 FLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIE 
               
               
                 QYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCIT 
               
               
                 CEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACE 
               
               
                 VLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYG 
               
               
                 RLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQ 
               
               
                 FFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDT 
               
               
                 TMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQL 
               
               
                 VHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKP 
               
               
                 NEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPA 
               
               
                 YKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILL 
               
               
                 QKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLH 
               
               
                 QQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEY 
               
               
                 LWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEA 
               
               
                 ARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGR 
               
               
                 REMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDD 
               
               
                 EGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQ 
               
               
                 ALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLE 
               
               
                 DRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAP 
               
               
                 SEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPV 
               
               
                 TFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHV 
               
               
                 MDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGV 
               
               
                 KFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQ 
               
               
                 LAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVI 
               
               
                 VAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVWLSLGCSDLGCAAPHSGWA 
               
               
                 GLTPAGPCSPCWSCRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKR 
               
               
                 SKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRG 
               
               
                 DFPTDCVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEF 
               
               
                 SYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAF 
               
               
                 IAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEER 
               
               
                 GWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHL 
               
               
                 VEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVK 
               
               
                 IADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKD 
               
               
                 PMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELV 
               
               
                 PQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTE 
               
               
                 PNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCE 
               
               
                 TSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK 
               
               
                   
               
               
                 SEQ ID NO: 7 is a nucleotide sequence that encodes a human myosin VIIa 
               
               
                 polypeptide; 
               
               
                 ATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAGATTGGGGCAGG 
               
               
                 AGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAGGTCCA 
               
               
                 GGTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCA 
               
               
                 CATCAAGCCTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTG 
               
               
                 GGGGACCTCAACGAGGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGACC 
               
               
                 ACCTCATCTACACGTATACGGGCTCCATCCTGGTGGCTGTGAACCCCTACCAGCT 
               
               
                 GCTCTCCATCTACTCGCCAGAGCACATCCGCCAGTATACCAACAAGAAGATTGGG 
               
               
                 GAGATGCCCCCCCACATCTTTGCCATTGCTGACAACTGCTACTTCAACATGAAAC 
               
               
                 GCAACAGCCGAGACCAGTGCTGCATCATCAGTGGGGAATCTGGGGCCGGGAAGA 
               
               
                 CGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCATCAGTGGGCAGCACTC 
               
               
                 GTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGCATTTGGGAAT 
               
               
                 GCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGACATCC 
               
               
                 ACTTCAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGG 
               
               
                 AAAAGTCACGTGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTA 
               
               
                 CTGCATGCTGGAGGGTATGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCA 
               
               
                 GGCCTCTGACTACAACTACTTGGCCATGGGTAACTGCATAACCTGTGAGGGCCGG 
               
               
                 GTGGACAGCCAGGAGTACGCCAACATCCGCTCCGCCATGAAGGTGCTCATGTTCA 
               
               
                 CTGACACCGAGAACTGGGAGATCTCGAAGCTCCTGGCTGCCATCCTGCACCTGGG 
               
               
                 CAACCTGCAGTATGAGGCACGCACATTTGAAAACCTGGATGCCTGTGAGGTTCTC 
               
               
                 TTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAGGTGAACCCCCCAGACCT 
               
               
                 GATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGACGGTGTCCACC 
               
               
                 CCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGGGATC 
               
               
                 TACGGGCGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGC 
               
               
                 CTCCCTCCCAGGATGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTT 
               
               
                 GGGTTTGAGAACTTTGCTGTGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCA 
               
               
                 ATGAGCACCTGCAGCAGTTCTTTGTGCGGCACGTGTTCAAGCTGGAGCAGGAGGA 
               
               
                 ATATGACCTGGAGAGCATTGACTGGCTGCACATCGAGTTCACTGACAACCAGGAT 
               
               
                 GCCCTGGACATGATTGCCAACAAGCCCATGAACATCATCTCCCTCATCGATGAGG 
               
               
                 AGAGCAAGTTCCCCAAGGGCACAGACACCACCATGTTACACAAGCTGAACTCCC 
               
               
                 AGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAACCATGAGACCCAGTT 
               
               
                 TGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCTTCCTGGAG 
               
               
                 AAGAACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAGGA 
               
               
                 ACAAGTTCATCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAG 
               
               
                 GAAGCGCTCGCCCACACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATG 
               
               
                 CGCACGCTGGGTGCCTGCCAGCCCTTCTTTGTGCGATGCATCAAGCCCAATGAGT 
               
               
                 TCAAGAAGCCCATGCTGTTCGACCGGCACCTGTGCGTGCGCCAGCTGCGGTACTC 
               
               
                 AGGAATGATGGAGACCATCCGAATCCGCCGAGCTGGCTACCCCATCCGCTACAGC 
               
               
                 TTCGTAGAGTTTGTGGAGCGGTACCGTGTGCTGCTGCCAGGTGTGAAGCCGGCCT 
               
               
                 ACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCATGGCTGAGGCTGTGCTGG 
               
               
                 GCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCTGAAGGACCACC 
               
               
                 ATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGTCATCC 
               
               
                 TCCTTCAGAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAA 
               
               
                 GAACGCTGCCACACTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAA 
               
               
                 CTACGGGCTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGG 
               
               
                 AAGCTGCACCAGCAGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCC 
               
               
                 GCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTC 
               
               
                 ACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCACCAACGCCTCA 
               
               
                 GGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGGAAG 
               
               
                 AGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGC 
               
               
                 AAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTG 
               
               
                 AAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAG 
               
               
                 GGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTC 
               
               
                 CTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTG 
               
               
                 AGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCC 
               
               
                 CTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGT 
               
               
                 TCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACCCGGCGGCCACT 
               
               
                 CAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCCCTGGC 
               
               
                 GGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCAC 
               
               
                 ACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAG 
               
               
                 ACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGC 
               
               
                 GAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTG 
               
               
                 CATTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTG 
               
               
                 CATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACC 
               
               
                 TCCAACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAGCAC 
               
               
                 TCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAA 
               
               
                 GAGCAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCC 
               
               
                 CCTCCGAGAAGTTTGTCAAGTACCTGCGGAACTTCATCCACGGGGGCCCGCCCGG 
               
               
                 CTACGCCCCGTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTCAATGGGACACGG 
               
               
                 ACACAGCCGCCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCAATC 
               
               
                 ATGTTGCCCGTGACATTCATGGATGGGACCACCAAGACCCTGCTGACGGACTCGG 
               
               
                 CAACCACGGCCAAGGAGCTCTGCAACGCGCTGGCCGACAAGATCTCTCTCAAGG 
               
               
                 ACCGGTTCGGGTTCTCCCTCTACATTGCCCTGTTTGACAAGGTGTCCTCCCTGGGC 
               
               
                 AGCGGCAGTGACCACGTCATGGACGCCATCTCCCAGTGCGAGCAGTACGCCAAG 
               
               
                 GAGCAGGGCGCCCAGGAGCGCAACGCCCCCTGGAGGCTCTTCTTCCGCAAAGAG 
               
               
                 GTCTTCACGCCCTGGCACAGCCCCTCCGAGGACAACGTGGCCACCAACCTCATCT 
               
               
                 ACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGAGAAGGAGG 
               
               
                 ACGACCTGGCTGAGCTGGCCTCCCAGCAGTACTTTGTAGACTATGGCTCTGAGAT 
               
               
                 GATCCTGGAGCGCCTCCTGAACCTCGTGCCCACCTACATCCCCGACCGCGAGATC 
               
               
                 ACGCCCCTGAAGACGCTGGAGAAGTGGGCCCAGCTGGCCATCGCCGCCCACAAG 
               
               
                 AAGGGGATTTATGCCCAGAGGAGAACTGATGCCCAGAAGGTCAAAGAGGATGTG 
               
               
                 GTCAGTTATGCCCGCTTCAAGTGGCCCTTGCTCTTCTCCAGGTTTTATGAAGCCTA 
               
               
                 CAAATTCTCAGGCCCCAGTCTCCCCAAGAACGACGTCATCGTGGCCGTCAACTGG 
               
               
                 ACGGGTGTGTACTTTGTGGATGAGCAGGAGCAGGTACTTCTGGAGCTGTCCTTCC 
               
               
                 CAGAGATCATGGCCGTGTCCAGCAGCAGGGAGTGCCGTGTCTGGCTCTCACTGGG 
               
               
                 CTGCTCTGATCTTGGCTGTGCTGCGCCTCACTCAGGCTGGGCAGGACTGACCCCG 
               
               
                 GCGGGGCCCTGTTCTCCGTGTTGGTCCTGCAGGGGAGCGAAAACGACGGCCCCCA 
               
               
                 GCTTCACGCTGGCCACCATCAAGGGGGACGAATACACCTTCACCTCCAGTAATGC 
               
               
                 TGAGGACATTCGTGACCTGGTGGTCACCTTCCTAGAGGGGCTCCGGAAGAGATCT 
               
               
                 AAGTATGTTGTGGCCCTGCAGGATAACCCCAACCCCGCAGGCGAGGAGTCAGGCT 
               
               
                 TCCTCAGCTTTGCCAAGGGAGACCTCATCATCCTGGACCATGACACGGGCGAGCA 
               
               
                 GGTCATGAACTCGGGCTGGGCCAACGGCATCAATGAGAGGACCAAGCAGCGTGG 
               
               
                 GGACTTCCCCACCGACTGTGTGTACGTCATGCCCACTGTCACCATGCCACCGCGG 
               
               
                 GAGATTGTGGCCCTGGTCACCATGACTCCCGATCAGAGGCAGGACGTTGTCCGGC 
               
               
                 TCTTGCAGCTGCGAACGGCGGAGCCCGAGGTGCGTGCCAAGCCCTACACGCTGGA 
               
               
                 GGAGTTTTCCTATGACTACTTCAGGCCCCCACCCAAGCACACGCTGAGCCGTGTC 
               
               
                 ATGGTGTCCAAGGCCCGAGGCAAGGACCGGCTGTGGAGCCACACGCGGGAACCG 
               
               
                 CTCAAGCAGGCGCTGCTCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGCAGGAG 
               
               
                 GCCTGCCTGGCCTTCATTGCTGTGCTCAAGTACATGGGCGACTACCCGTCCAAGA 
               
               
                 GGACACGCTCCGTCAACGAGCTCACCGACCAGATCTTTGAGGGTCCCCTGAAAGC 
               
               
                 CGAGCCCCTGAAGGACGAGGCATATGTGCAGATCCTGAAGCAGCTGACCGACAA 
               
               
                 CCACATCAGGTACAGCGAGGAGCGGGGTTGGGAGCTGCTCTGGCTGTGCACGGG 
               
               
                 CCTTTTCCCACCCAGCAACATCCTCCTGCCCCACGTGCAGCGCTTCCTGCAGTCCC 
               
               
                 GAAAGCACTGCCCACTCGCCATCGACTGCCTGCAACGGCTCCAGAAAGCCCTGAG 
               
               
                 AAACGGGTCCCGGAAGTACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAGCA 
               
               
                 CAAGACCACCCAGATTTTCCACAAGGTCTACTTCCCTGATGACACTGACGAGGCC 
               
               
                 TTCGAAGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCACCA 
               
               
                 GGCTGCTCCTCAAGTCCTCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGACAA 
               
               
                 GGTCATCAGCGTTCCTGAGAATGACTTCTTCTTTGACTTTGTTCGACACTTGACAG 
               
               
                 ACTGGATAAAGAAAGCTCGGCCCATCAAGGACGGAATTGTGCCCTCACTCACCTA 
               
               
                 CCAGGTGTTCTTCATGAAGAAGCTGTGGACCACCACGGTGCCAGGGAAGGATCCC 
               
               
                 ATGGCCGATTCCATCTTCCACTATTACCAGGAGTTGCCCAAGTATCTCCGAGGCTA 
               
               
                 CCACAAGTGCACGCGGGAGGAGGTGCTGCAGCTGGGGGCGCTGATCTACAGGGT 
               
               
                 CAAGTTCGAGGAGGACAAGTCCTACTTCCCCAGCATCCCCAAGCTGCTGCGGGAG 
               
               
                 CTGGTGCCCCAGGACCTTATCCGGCAGGTCTCACCTGATGACTGGAAGCGGTCCA 
               
               
                 TCGTCGCCTACTTCAACAAGCACGCAGGGAAGTCCAAGGAGGAGGCCAAGCTGG 
               
               
                 CCTTCCTGAAGCTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTCGAGGTG 
               
               
                 AAGCAAACTACGGAGCCAAACTTCCCTGAGATCCTCCTAATTGCCATCAACAAGT 
               
               
                 ATGGGGTCAGCCTCATCGATCCCAAAACGAAGGATATCCTCACCACTCATCCCTT 
               
               
                 CACCAAGATCTCCAACTGGAGCAGCGGCAACACCTACTTCCACATCACCATTGGG 
               
               
                 AACTTGGTGCGCGGGAGCAAACTGCTCTGCGAGACGTCACTGGGCTACAAGATG 
               
               
                 GATGACCTCCTGACTTCCTACATTAGCCAGATGCTCACAGCCATGAGCAAACAGC 
               
               
                 GGGGCTCCAGGAGCGGCAAGTGA 
               
               
                   
               
               
                 SEQ ID NO: 8 is an amino acid sequence of a human myosin VIIa polypeptide 
               
               
                 (isoform 2); 
               
               
                 MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATH 
               
               
                 IKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIY 
               
               
                 SPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQ 
               
               
                 FLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIE 
               
               
                 QYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCIT 
               
               
                 CEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACE 
               
               
                 VLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYG 
               
               
                 RLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQ 
               
               
                 FFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDT 
               
               
                 TMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQL 
               
               
                 VHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKP 
               
               
                 NEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPA 
               
               
                 YKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILL 
               
               
                 QKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLH 
               
               
                 QQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEY 
               
               
                 LWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEA 
               
               
                 ARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGR 
               
               
                 REMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDD 
               
               
                 EGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQ 
               
               
                 ALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLE 
               
               
                 DRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAP 
               
               
                 SEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPV 
               
               
                 TFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHV 
               
               
                 MDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGV 
               
               
                 KFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQ 
               
               
                 LAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVI 
               
               
                 VAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSN 
               
               
                 AEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQV 
               
               
                 MNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLL 
               
               
                 QLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQA 
               
               
                 LLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEA 
               
               
                 YVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCL 
               
               
                 QRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFC 
               
               
                 QNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSL 
               
               
                 TYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYR 
               
               
                 VKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFL 
               
               
                 KLIFKWPTFGSAFFEQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGN 
               
               
                 TYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK 
               
            
           
         
       
     
     CMv1 Overlap Vector System 
     Some embodiments contemplate the overlapping vector system as described herein, with one or more substitutions made in the 3′ untranslated region downstream of the MYO7A partial coding sequence, and before the 3′ AAV inverted terminal repeat. In some embodiments, these substitutions are intended to remove potential (or putative) in-frame stop codons. In some embodiments, these substitutions remove one or more putative stop codons in a non-coding sequence. In particular embodiments, the substitutions remove one or more putative stop codons in the 3′ untranslated region between the partial coding sequence encoding the C-terminal part of the polypeptide and the 3′ AAV inverted terminal repeat of the second AAV vector polynucleotide (e.g., downstream of the MYO7A N-terminal fragment). In some embodiments, the one or more putative stop codons are removed and replaced with a “stuffer” sequence (see  FIG.  43   ). In some embodiments, the first AAV vector polynucleotide thus comprises a partial coding sequence that does not encode the single-alpha helix (SAH) domain of the selected full-length polypeptide. 
     As a result of these substitutions, a front-half vector is created having the nucleotide sequence comprising SEQ ID NO: 36. In such embodiments, overlap vector systems of the disclosure may comprise: 
     (i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a selected full-length polypeptide; and 
     (ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a partial coding sequence that encodes a C-terminal part of the selected full-length polypeptide. In some embodiments, the second AAV vector polynucleotide is followed by a polyadenylation (pA) signal sequence. The coding sequences in the first and second vectors when combined encode the selected full-length polypeptide, or a functional fragment or variant thereof. The polypeptide encoding sequence in the first and second AAV vectors comprises sequence that overlaps. 
     In some embodiments, the C-terminal part of the selected full-length polypeptide (e.g., the myosin polypeptide) comprises the single-alpha helix (SAH) domain of the selected full-length polypeptide. 
     In some embodiments, the polynucleotide sequence that overlaps comprises SEQ ID NO: 39. In particular embodiments, the sequence overlap between the first and second AAV vectors is between about 1 and about 500 nucleotides; between about 100 and about 200 nucleotides; between about 200 and about 300 nucleotides; or between about 300 and about 400 nucleotides. In particular embodiments, the sequence overlap of the second generation overlap vector system is about 350 nucleotides. In an exemplary embodiment, the sequence overlap of the second generation overlap vector system is 361 nucleotides. 
     In some embodiments, the polynucleotide sequence that overlaps does not comprise any portion of exon 23 of the hMYO7A gene. In some embodiments, the polynucleotide sequence that overlaps does not comprise exon 23 in full (e.g., 100% of exon 23). In some embodiments, the polynucleotide sequence that overlaps comprises a portion of exon 17, exon 18 in full, exon 19 in full, exon 20 in full, and a portion of exon 21 of the hMYO7A gene. In some embodiments, the polynucleotide sequence that overlaps comprises a portion of exon 17, a portion of exon 18, a portion of exon 19, a portion of exon 20, and/or a portion of exon 21 of the hMYO7A gene. As used herein, a “portion” may comprise e.g. at least about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%, etc. of the exon and/or intron. 
     In an exemplary embodiment, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 36, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 38, or a functional fragment and/or variant thereof. 
     In some embodiments, first AAV vector polynucleotide comprises a partial coding sequence that does not encode the single-alpha helix (SAH) domain of the selected full-length (e.g. myosin) polypeptide. In some embodiments, the second AAV vector polynucleotide is followed by a polyadenylation (pA) signal sequence. 
     In some embodiments, any vector of the overlap polynucleotide vector systems described in the disclosure may be administered by parenteral administration, such as intravenous, intramuscular, intraocular, intranasal, etc. The vector can be administered in vivo, in vitro or ex vivo. 
     In some embodiments, any vector of the overlap polynucleotide vector systems described herein may be administered to the eye. In particular embodiments, a vector is administered to the eye of a subject by subretinal injection. In some embodiments, any vector of the hybrid polynucleotide vector systems described herein may be administered to the ear. 
     In some embodiments, any vector of the hybrid polynucleotide vector systems described herein the polynucleotide vector system is administered to the ear of a subject, e.g., by a round window injection or during cochlear implant surgery. 
     SEQ ID NO: 5 is a nucleotide sequence encoding a human myosin VIIa polypeptide (protein coding sequence is nucleotides 273-6920), the sequence of which is disclosed herein; 
     SEQ ID NO: 6 is the amino acid sequence of the human myosin VIIa polypeptide encoded by nucleotides 273-6920 of SEQ ID NO: 5, the sequence of which is disclosed herein; 
     SEQ ID NO: 7 is a nucleotide sequence that encodes a human myosin VIIa polypeptide, the sequence of which is disclosed herein; 
     SEQ ID NO: 8 is an amino acid sequence of a human myosin VIIa polypeptide (isoform 2), the sequence of which is disclosed herein. 
     Some aspects of the disclosed overlap vectors contemplate a virus or a recombinant viral particle comprising the first AAV vector polynucleotide or the second AAV vector polynucleotide as described herein. In particular embodiments, the first AAV vector polynucleotide comprises SEQ ID NO: 36, and the second AAV vector polynucleotide comprises SEQ ID NO: 38. In some embodiments, the virus or recombinant viral particle is characterized as an adeno-associated virus (AAV) or an infectious AAV viral particle. In some embodiments, the recombinant AAV viral particle includes one or more tyrosine-to-phenylalanine (Y-F) mutations in a capsid protein of the virus or virion. Tyrosine-to-phenylalanine (Y-F) mutations in a capsid protein of the virus or virion at amino acid position 733 are specifically contemplated herein (for example, AAV8 Y733F). 
     In some embodiments, the virus or virion is packaged in an AAV5, AAV7, AAV8, AAV9, AAV44.9, AAV44.9(E531D), AAV2(4pMut), AAVAnc80, AAVrh.8, AAVrh.8R, AAV9-PHP.B, AAV9-PHP.eB, AAVrh.10, or AAVrh.74 capsid. In some embodiments, the viral particle comprises an AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV7m8, AAV-DJ, AAV2/2-MAX, AAVSHh10, AAVSHh10Y, AAV3b, AAVLK03, AAV8PB2, AAV1(E531K), AAV6(D532N), AAV6-3pmut, AAV2G9, AAV44.9, AAV44.9(E531D), AAVrh.8, AAVrh.8R, AAV9-PHP.B, and/or AAVAnc80 capsid. In exemplary embodiments, the virion is packaged in an AAV44.9(E531D) capsid variant. 
     In some embodiments, the overlap polynucleotide vector systems described herein use a tissue-specific promoter. In some embodiments, the systems use a promoter that mediates expression in the eye. In some embodiments, the systems use a promoter that mediates expression in the ear. 
     In some embodiments, the overlap polynucleotide vector systems described herein use any one of the following promoters: a cytomegalovirus (CMV) promoter, an elongation factor-1 alpha (EF-1 alpha) promoter, a cone arrestin promoter, a chimeric CMV β actin promoter (CBA), a truncated chimeric CMV β actin (smCBA), a human myosin 7a gene-derived promoter, a cone transducin a (TαC) gene-derived promoter, a rhodopsin promoter, a cGMP-phosphodiesterase β-subunit promoter, human or mouse rhodopsin promoter, a human rhodopsin kinase (hGRK1) promoter, a synapsin promoter, a glial fibrillary acidic protein (GFAP) promoter, a rod specific IRBP promoter, a RPE-specific vitelliform macular dystrophy-2 [VMD2] promoter, and combinations thereof. In some embodiments, the polynucleotide vector system described herein uses a human rhodopsin kinase (hGRK1) promoter. In some embodiments, the polynucleotide vector system uses a cone arrestin promoter. 
     In some embodiments for delivery to the eyes (retina) of a subject, the disclosed overlap polynucleotide vector systems use a cytomegalovirus (CMV) promoter. In some embodiments, the polynucleotide vector system uses an EF-1 alpha promoter. In some embodiments for delivery to the ears (hair cells) of a subject, the polynucleotide vector system uses a synapsin or GFAP promoter (see Lee et al., Hearing Research). 
     Hybrid Vector Systems 
     In some aspects, hybrid dual AAV vector systems are provided. These hybrid vector systems drive higher levels of full length MYO7A than overlap vectors and produce truncated protein from the front half vector. The hybrid front half vector leads to reduced retinal function in subretinally injected mice. (See  FIGS.  15 B-E .) In various embodiments, the hybrid vector systems of the disclosure do not produce a truncated MYO7A protein fragment following administration to a mouse or a subject. 
     Altering the split point, codon modifying the front half vector, and/or minimizing the length of the back half vector leads to production of full-length MYO7A at levels equal to or above that seen with the first generation hybrid vectors. The improved vectors provided herein generate far less undesired truncated protein side product. from the front half vector. In some embodiments, production of the truncated protein is eliminated, partially or completely. 
     In some aspects of the disclosure, a hybrid vector system of the disclosure includes: 
     (i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end (for example, the 5′-end and the 3′-end) of the polynucleotide, and between the inverted terminal repeats a suitable promoter followed by (for example, 3′ to the promoter) a partial coding sequence that encodes an N-terminal part of a selected full-length polypeptide followed by a splice donor site and an intron, and 
     (ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end (5′-end and 3′-end) of the polynucleotide, and between the inverted terminal repeats an intron and a splice acceptor site for the intron, optionally followed by a partial coding sequence that encodes a C-terminal part of the selected full-length polypeptide, optionally followed by a polyadenylation (pA) signal sequence. The intron sequence in the first and second AAV vectors comprises sequence that overlaps. 
     In some embodiments, the split point between the first and second AAV vector polynucleotide sequences is between exon 21 and exon 22 of the hMYO7A gene. 
     In an exemplary embodiment, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 31, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 32, or a functional fragment and/or variant thereof. 
     The coding sequences in the first and second vectors when combined encode the selected full-length polypeptide, or a functional fragment or variant thereof. In some embodiments, the selected full-length polypeptide is hMYO7A. In some embodiments, the selected full-length polypeptide is hMYO7B. In some embodiments, the selected full-length polypeptide is isoform II of hMYO7A. In some embodiments, the polynucleotide sequence corresponding to the tail domain of the MYO7A protein is removed from the first AAV vector polynucleotide. 
     It will be appreciated that this disclosure is not limited to delivery of full-length myosin 7A polypeptide or myosin 7A-encoding nucleotides. In some embodiments, the selected full-length polypeptide is selected from ABCA4 (Stargardt disease), CEP290 (LCA10), EYS (Retinitis Pigmentosa), RP1 (Retinitis Pigmentosa), ALMS1 (Alstrom syndrome), CDH23 (Usher syndrome 1D), PCDH15 (Usher syndrome 1F), and USH2A (Usher syndrome 2A). In some embodiments, the selected full-length polypeptide is selected from DMD (Duchenne muscular dystrophy), CFTR (Cystic fibrosis), GDE (Glycogen storage disease III), DYSF (dysferlinopathies), OTOF (neurosensory nonsyndromic recessive deafness) and F8 (Hemophilia A). In some embodiments, the selected full-length polypeptide is not OTOF. 
     In some embodiments, all or part of the intron sequence present at the 3′-end of the coding sequence of the first vector is identical or substantially identical with all or part of the intron sequence present at the 5′-end of the coding sequence of the second vector. In some embodiments, intron sequence overlap between the first and second AAV vectors is several hundred nucleotides in length. In particular embodiments, the intron sequence overlap is about 50 to about 500 nucleotides or so in length; alternatively between about 200 and about 300 nucleotides or so in length. 
     In particular embodiments, the intron sequence utilized in any vector system of the disclosure is a sequence of an intron naturally present in the genomic sequence of the gene encoding the selected polypeptide. In some embodiments, characterized as the native intron hybrid vectors, the intron is intron 23 of the hMYO7A gene. In particular embodiments, the polypeptide encoded is hMYO7A, or a functional fragment thereof, and the intron is a partial sequence of full intron 23 of the hMYO7A gene. In particular embodiments, the polypeptide encoded is hMYO7A, or a functional fragment thereof, and the intron is the full intron 23 of the hMYO7A gene. 
     In some embodiments of the native intron hybrid vectors as described herein, the recombinogenic sequence of the dual vector system comprises partial sequences of exon 21, exon 22, and/or exon 23 of the hMYO7A gene. In some embodiments of the native intron hybrid vectors as described herein, the dual vector system comprising partial sequences of exon 21, exon 22, and/or exon 23 of the hMYO7A gene utilizes a partial sequence of a full native intron. In some embodiments, the native intron is intron 23 of the hMYO7A gene. Thus, in some embodiments, the intron sequence is a partial sequence of full intron 23 of the hMYO7A gene. As used herein, a “partial sequence” may comprise e.g. at least about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%, etc. of the exon and/or intron sequence. In some embodiments, the first and/or second intron sequence comprises a sequence of an intron naturally present in the genomic sequence of the gene encoding a myosin polypeptide. In some embodiments, the intron sequence is a partial sequence of full intron 23 of the hMYO7A gene. 
     A number of strategies have been devised to overcome the issue of random concatemerization and thereby increase specificity as well as efficiency of dual vector platforms. First, the addition of a highly-recombinogenic sequence such as that used in the AP hybrid vector here has resulted in significantly increased protein expression compared with the trans-splicing system. Ghosh et al. (2011) provide a detailed analysis of the 270-bp AP sequence used in this study as well as other sequences derived from AP that direct recombination and lead to significant improvement over trans-splicing vectors. The finding that AP hybrid vectors are more efficient than trans-splicing vectors supports that the AP sequence directs at least some of the concatemerization events toward the proper orientation with recombination then occurring via this sequence or via the ITRs. The APhead domain in particular can mediate appropriate head-to-tail concatemerization following re-combination of the dual vectors in the cell. Regardless, with more concatemers properly aligned, the AP hybrid system mediates a more-efficient expression of MYO7A. (Another approach for directing concatemerization is the use of single-strand oligonucleotides that are capable of tethering the back end of the 5′ vector and the front end of the 3′ vector together (Hirsch et al., 2009); however, this strategy requires efficient delivery of the oligonucleotide to the nucleus of the target cells timed with the dual vectors.) Finally, dual vectors utilizing mismatched ITRs can be used to direct concatemerization in a head-to-tail orientation (Yan et al., 2005), although the process may require further optimization of the AAV packaging machinery. 
     Accordingly, in some embodiments, the intron sequence utilized in the vector system of the disclosure is a sequence of an intron that is not naturally present in the genomic sequence of a gene encoding the selected polypeptide. In particular embodiments, the intron is a synthetic alkaline phosphatase (AP) intron. The intron sequences utilized in the vector system of the disclosure can comprise splice donor and splice acceptor sequences. In some embodiments, the intron sequence is a recombinogenic, intronic sequence (for example, the AK sequence of the F1 phage as shown in Trapani, et al. 2014). In these embodiments, the hybrid vectors, characterized as the second generation hybrid vectors described herein, rely on both ITR-mediated concatemerization and homologous recombination mediated by the AK sequence for the reconstitution of the full-length expression cassette. Thus, in some embodiments, the intron sequence is the AK sequence of the F1 phage. Accordingly, in some embodiments of the disclosed hybrid vectors, the vectors comprise one or more AP intronic spliceosome recognition sites, such as one or more AP splice acceptor (APSA) domains or AP splice donor (APSD) domains. In exemplary embodiments, these vectors comprise an APSA and an APSD. In some embodiments, the front half vector contains an APSA and the back half vector contains an APSD. In some embodiments, the front half vector contains an APSD and the back half vector contains an APSA. See  FIGS.  37 A and  45 A . 
     Accordingly, in exemplary embodiments, the hybrid vector pairs contain an APhead-encoding sequence as part of the AP intron. In some embodiments of the disclosed hybrid vectors, the vectors comprise an intronic sequence comprising a nucleotide sequence having at least 85%, 90%, 92.5%, 95%, 98%, or 99% identity to either of SEQ ID NO: 69 or 70. In some embodiments of the disclosed hybrid vectors, the vectors comprise the nucleotide sequence of SEQ ID NO: 69 or 70 (APhead sequence). 
     Polypeptides other than hMYO7A that are contemplated for delivery using any of the disclosed hybrid vectors include, but are not limited to, harmonin (Uniprot Q9Y6N9), cadherin 23 (Uniprot Q9H251), protocadherin 15 (Uniprot Q96QU1), and usherin (USH2A) (Uniprot 075445). In some embodiments, the selected full-length polypeptide is encoded by a gene of about 5 Kb to about 10 Kb in length. In some embodiments, the selected full-length polypeptide is encoded by a gene of about 6 Kb to about 9 Kb in length. In some embodiments, the selected full-length polypeptide is encoded by a gene of about 7 Kb to about 8 Kb in length. In some embodiments, hybrid dual vectors expressing portions (or halves) of a large gene contain a sequence between the first intron and second intron of the first and second AAV vector polynucleotides, respectively; and a large gene other than MYO7A and that comprises a nucleotide sequence having at least 80%, 85%, 90%, 95%, 98%, or 99% identity to any one of SEQ ID NOs: 39 and 52-59. In some embodiments, these hybrid vectors an intronic sequence containing the nucleotide sequence of any one of SEQ ID NOs: 39 and 53-59, e.g., SEQ ID NO: 56 or 57. The large gene may be selected from ABCA4, CEP290, EYS, RP1, ALMS1, CDH23, PCDH15, USH1C, USH1G, USH2A, DNFB31, DMD, CFTR, GDE, DYSF, F8, and DFNB2. These hybrid vectors encoding non-MYO7A (e.g., ABCA4) genes may contain an overlapping region identified through the improved overlap vectors provided herein as the recombinogenic sequence, in place of the recombinogenic APhead sequence/domain. The overlapping regions of these hybrid vectors are flanked by splice acceptor and/or splice donor sequences, such that the overlapping region is spliced out, and does not code for any MYO7A protein. 
     In some embodiments, the hybrid vector system of the first generation contains a split point between exons 23 and 24, wherein the sequence corresponding to the tail domain of the MYO7A protein is contained within the front-half vector represented by SEQ ID NO: 3. The back-half vector of this exon 23/24 hybrid vector system is shown in SEQ ID NO: 4. In some embodiments, the second generation hybrid vector system contains a split point located between exons 21 and 22, wherein the sequence corresponding to the tail domain of the MYO7A protein is removed from the front-half vector of the exon 23/24 hybrid vector system, thereby generating the second generation hybrid front-half vector (SEQ ID NO: 31). 
     Thus, in an exemplified embodiment, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 31, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 32, or a functional fragment and/or variant thereof, and the intronic sequence is the AK sequence of the F1 phage. 
     In some embodiments, the split point between the first and second AAV vector polynucleotide sequences is between exon 21 and exon 22 of the hMYO7A gene. In some embodiments, the split point between the first and second AAV vector polynucleotide sequences is between exon 22 and exon 23 of the hMYO7A gene. 
     In some embodiments, the split point between the first and second AAV vector polynucleotide sequences is not between exon 23 and exon 24 of the hMYO7A gene. In exemplary embodiments, the hybrid vectors of the disclosure do not comprise any of the nucleotide sequences of SEQ ID NOs: 3 and 4. 
     CMv1 Hybrid Vector System 
     Some embodiments contemplate a hybrid vector system as described herein, wherein substitutions are made in a noncoding sequence of the vector (e.g., the 3′ untranslated region (3′ UTR) downstream of the MYO7A partial coding sequence, and before the 3′ AAV inverted terminal repeat of the inverted terminal repeat pairs of the first and/or second vector polynucleotide). In some embodiments, the substitutions are positioned in putative stop codons, such that these potential stop codons are removed. In some embodiments, one or more potential stop codons are removed by installing one or more nucleotide substitutions in the alkaline phosphatase (AP) intronic splice donor sequence (“AP intron”) of the front-half vector (for example, the front-half vector comprising SEQ ID NO: 31). In some embodiments, three potential stop codons are modified within the alkaline phosphatase intronic splice donor sequence of the front-half vector. As a result of the modification of these putative stop codons in the APhead sequence, a modified front-half vector is created comprising SEQ ID NO: 33. See  FIG.  42   . 
     Accordingly, in some embodiments, a hybrid vector system of the disclosure comprises: 
     i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a selected full-length polypeptide followed by a splice donor site and an intron; and 
     ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats an intron and a splice acceptor site for the intron, and optionally followed by a partial coding sequence that encodes a C-terminal part of the selected full-length polypeptide, followed by a polyadenylation (pA) signal sequence. The intron sequence in the first and second AAV vectors comprises sequence that overlaps. In an exemplary embodiment, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 33, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 32, or a functional fragment and/or variant thereof. 
     CMv2 Hybrid Vector System 
     Still other embodiments contemplate the hybrid vector system as described herein, with one additional putative in-frame stop codon modified in the front-half vector (for example, the front-half vector comprising SEQ ID NO: 33). In some embodiments, the modification comprises the installation of a substitution into the APhead sequence of the front-half vector that removes a putative stop codon from this sequence. As a result of this modification and removal of this putative stop codon, a further modified front-half vector is created comprising SEQ ID NO: 34. 
     Upon modification of the additional putative in-frame stop codon as described herein, some embodiments consider making complementary changes to the back-half vector (for example, the back-half vector comprising SEQ ID NO: 32), where the identical codon is also modified in the back-half vector. Thus, in some embodiments, the modification comprises the installation of a substitution into the APhead sequence of the back-half vector that removes a putative stop codon from this sequence. As a result of the modification of this additional stop codon, a modified back-half vector is created comprising SEQ ID NO: 35. See  FIG.  42   . 
     As such, in the CMv1 vector, three potential in-frame stop codons in the AP intron are removed. In the CMv2 vector, these same three potential stop codons in the AP intron were removed, and one potential stop codon from the APhead coding sequence was removed. 
     Therefore, in particular embodiments, a hybrid vector system of the disclosure comprises: 
     i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a selected full-length polypeptide followed by a splice donor site and an intron; and 
     ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats an intron and a splice acceptor site for the intron, and optionally followed by a partial coding sequence that encodes a C-terminal part of the selected full-length polypeptide, followed by a polyadenylation (pA) signal sequence. The intron sequence in the first and second AAV vectors comprises sequence that overlaps. In an exemplary embodiment, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 34, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 35, or a functional fragment and/or variant thereof. 
     V2 MIN and CMv2 MIN Hybrid Vector Systems (Also Known as CMv2.1 Hybrid Vector System) 
     When the split point is altered from the junction between exons 23 and 24, as in the first-generation hybrid vector system, to the junction between exons 21 and 22, as in the second-generation hybrid vector system, additional portions of the MYO7A sequence are shifted to the back-half second-generation hybrid vector. Because of this, in some embodiments the second-generation back-half hybrid vector is close to, but does not exceed, the AAV packaging limit. Accordingly, in some embodiments, the modified back-half vector comprising SEQ ID NO: 35 may be further modified to remove any residual (extra), non-essential sequences, such as restriction enzyme sites and tag sequences, from the 3′ end of the construct. These residual sequences are sometimes referred to as “unseeded legacy” sequences. The resulting modified back-half vector comprises SEQ ID NO: 49. This vector system is known as the “V2 MIN” or “V2-back MIN” hybrid system. In some embodiments, this system contains an HA tag (“V2 MIN HA”). The V2 MIN HA vector is set forth as SEQ ID NO: 48. The V2 MIN back-half vector is 122 bp shorter than the first-generation hybrid back-half vectors (4981 bp vs. 4861 bp). 
     In some embodiments, a vector is provided in which unseeded legacy sequences are removed, and one or more substitutions in non-coding sequences are installed. In some embodiments, these one or more substitutions are positioned in putative stop codons, such that these potential stop codons are removed. In some embodiments, one or more potential stop codons are removed by installing one or more nucleotide substitutions in the APhead sequence of the front-half vector. In some embodiments, the one or more putative stop codons are removed and replaced with a “stuffer” sequence (see  FIG.  43   ). 
     In some embodiments, three potential stop codons are modified within the alkaline phosphatase intron sequence of the front-half vector. In some embodiments, one putative stop codon is modified (removed) by installing one or more substitutions in the APhead sequence of the front-half vector. As a result of the modification of these putative stop codons in the APhead sequence, the modified front-half vector of SEQ ID NO: 34 was generated. 
     In some embodiments, one putative stop codon is likewise modified in the APhead sequence of the back-half vector. As a result of the modification of this putative stop codon in the APhead sequence, the modified back-half vector of SEQ ID NO: 44 was generated. 
     This vector system is known as the CMv2 MIN system. In some embodiments, this system contains an HA tag (“CMv2 MIN HA”). The CMv2 MIN HA vector is set forth as SEQ ID NO: 47. The CMv2 MIN back-half vector is 121 bp shorter than the first-generation hybrid back-half vectors (4982 bp vs. 4861 bp). 
     Thus, in some embodiments, a hybrid vector system of the disclosure comprises: 
     i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a selected full-length polypeptide followed by a splice donor site and an intron; and 
     ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats an intron and a splice acceptor site for the intron, and optionally followed by a partial coding sequence that encodes a C-terminal part of the selected full-length polypeptide, followed by a polyadenylation (pA) signal sequence. The intron sequence in the first and second AAV vectors comprises sequence that overlaps. In an exemplary embodiment, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 34, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 44, or a functional fragment and/or variant thereof. In some embodiments, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 34, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 47, or a functional fragment and/or variant thereof. In some embodiments, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 34, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 48, or a functional fragment and/or variant thereof. 
     CMv3 MIN Hybrid Vector Systems 
     Still other embodiments contemplate the hybrid vector system as described herein, with three additional in-frame stop codons modified in the front-half vector. In some embodiments, one or more nucleotide substitutions (e.g., three substitutions) are made in the 3′UTR or ITR downstream of the MYO7A coding sequence. Accordingly, in some embodiments, a front-half vector is provided in which one in-frame stop codon in the APhead sequence, three in-frame stop codons in the AP intron sequence, and three in-frame stop codons in the 3′ UTR sequence have been removed through the installation of substitutions. As a result of the modification of these putative stop codons, a further modified front-half vector is created comprising SEQ ID NO: 46 (“AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead-CMv3”, or simply “CMv3 hybrid system”). In exemplary embodiments, the CMv3 hybrid system is a CMv3 MIN system in that residual, unseeded legacy sequences (e.g., restriction enzyme sites) have been removed. In some embodiments, the CMv3 system has a HA tag. 
     Therefore, in an exemplary embodiment, the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 46, or a functional fragment and/or variant thereof, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 35, or a functional fragment and/or variant thereof. 
     Thus, in some embodiments, the polynucleotide vector system of the disclosure is a CMv3 hybrid system. In some embodiments, the vector system is a CMv3 MIN system. In some embodiments, the vector system is a CMv2 system. In some embodiments, the vector system is a CMv2 MIN system. In some embodiments, the vector system is a CMv1 or CMv1 MIN system. Any of the disclosed front-half hybrid vectors may be combined with any of the disclosed back-half hybrid vectors in the compositions of the disclosure. 
     In exemplary embodiments, polynucleotide vector systems are provided that comprise: 
     i) a first AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats a promoter followed by a partial coding sequence that encodes an N-terminal part of a myosin polypeptide followed by a splice donor site and an intron, and 
     ii) a second AAV vector polynucleotide comprising an inverted terminal repeat at each end of the polynucleotide, and between the inverted terminal repeats an intron and a splice acceptor site for the intron, 
     wherein the intron sequence in the first and second AAV vectors comprises a polynucleotide sequence that overlaps, and 
     wherein the first AAV vector polynucleotide comprises a nucleotide sequence selected from SEQ ID NOs: 31, 33, 34, and 46; 
     and the second AAV vector polynucleotide comprises a nucleotide sequence selected from SEQ ID NOs: 32, 35, 44, and 47-49. 
     In some embodiments of the hybrid vector systems described herein, the selected full-length polypeptide is a myosin polypeptide. In some embodiments, the myosin polypeptide is human myosin VII A (hMYO7A). In some embodiments, the myosin polypeptide is human myosin VII B (hMYO7B). In some embodiments, the myosin polypeptide is myosin 7 (VII) isoform II. In some embodiments, the myosin polypeptide is another myosin isoform or a functional fragment thereof. In particular embodiments, full-length myosin 7A or isoform II is encoded in the provided vector systems. 
     In some embodiments, the C-terminal part of the selected full-length polypeptide (e.g., the myosin polypeptide) comprises the single-alpha helix (SAH) domain of the selected full-length polypeptide. 
     The coding sequences in the first and second vectors when combined encode the selected full-length polypeptide, or a functional fragment or variant thereof. Accordingly, in some embodiments, all or part of the intron sequence present at the 3′-end of the coding sequence of the first vector is identical or substantially identical with all or part of the intron sequence present at the 5′-end of the coding sequence of the second vector. 
     Some embodiments of the hybrid vectors described herein contemplate a virus or a recombinant viral particle comprising the first AAV vector polynucleotide or the second AAV vector polynucleotide as described herein. In particular embodiments, the first AAV vector polynucleotide comprises SEQ ID NO: 33, and the second AAV vector polynucleotide comprises SEQ ID NO: 32. In some embodiments, the virus or recombinant viral particle is characterized as an adeno-associated virus (AAV) or an infectious AAV viral particle. In some embodiments, the recombinant AAV viral particle includes one or more tyrosine-to-phenylalanine (Y-F) mutations in a capsid protein of the virus or virion. Tyrosine-to-phenylalanine (Y-F) mutations in a capsid protein of the virus or virion at amino acid position 733 are specifically contemplated herein (for example, AAV8 Y733F). Likewise, tyrosine-to-phenylalanine (Y-F) mutations in a capsid protein of the virus or virion at amino acid position 731 are specifically contemplated herein (for example, AAV44.9(Y73IF)). 
     In some embodiments, the virus or virion is packaged in an AAV5, AAV7, AAV8, AAV9, AAV44.9, AAV44.9(E531D), AAV2(4pMut), AAVAnc80, AAVrh.8, AAVrh.8R, AAV9-PHP.B, AAV9-PHP.eB, AAVrh.10, or AAVrh.74 capsid. In some embodiments, the viral particle comprises an AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV7m8, AAV-DJ, AAV2/2-MAX, AAVSHh10, AAVSHh10Y, AAV3b, AAVLK03, AAV8PB2, AAV1(E531K), AAV6(D532N), AAV6-3pmut, AAV2G9, AAV44.9, AAV44.9(E531D), AAVrh.8, AAVrh.8R, AAV9-PHP.B, or an AAVAnc80 capsid. In exemplary embodiments for delivery to the retina, the virion is packaged in an AAV44.9(E531D) capsid variant. In exemplary embodiments for delivery to the hair cells of the ear, the virion is packaged in an AAV9-PHP.B capsid variant. 
     In some embodiments, the hybrid polynucleotide vector systems described herein use a tissue-specific promoter. In some embodiments, the systems use a promoter that mediates expression in the eye. In some embodiments, the systems use a promoter that mediates expression in the ear. 
     In some embodiments, the hybrid polynucleotide vector systems described herein use any one of the following promoters: a cytomegalovirus (CMV) promoter, an elongation factor-1 alpha (EF-1 alpha) promoter, a cone arrestin promoter, a chimeric CMV β actin (smCBA) promoter, a human myosin 7a gene-derived promoter, a cone transducin a (TαC) gene-derived promoter, a rhodopsin promoter, a cGMP-phosphodiesterase β-subunit promoter, human or mouse rhodopsin promoter, a human rhodopsin kinase (hGRK1) promoter, a synapsin promoter, a glial fibrillary acidic protein (GFAP) promoter, a rod specific IRBP promoter, a RPE-specific vitelliform macular dystrophy-2 [VMD2] promoter, and combinations thereof. In some embodiments, the polynucleotide vector system described herein uses a human rhodopsin kinase (hGRK1) promoter. In some embodiments, the polynucleotide vector system uses a cone arrestin promoter. In some embodiments, the polynucleotide vector system uses a cytomegalovirus (CMV) promoter. In some embodiments for delivery to the eyes (retina) of a subject, the disclosed overlap polynucleotide vector systems use a cytomegalovirus (CMV) promoter. 
     In some embodiments for delivery to the retina, the hybrid polynucleotide vector system uses an EF-1 alpha promoter. In some embodiments for delivery to the ears (hair cells) of a subject, the polynucleotide vector system uses a synapsin or GFAP promoter (see Lee et al., Hearing Research). 
     Each embodiment contained with the “hybrid vectors” section and as described herein is specifically contemplated for each hybrid vector system described, for example the vector system wherein the split point between the first and second AAV vector polynucleotide sequences is between exon 21 and exon 22 of the hMYO7A gene; the vector system wherein the split point between the first and second AAV vector polynucleotide sequences is between exon 22 and exon 23 of the hMYO7A gene; the vector system with a front-half vector comprising the nucleotide sequence of SEQ ID NO: 31 and a back-half vector comprising the nucleotide sequence of SEQ ID NO: 32; the vector system with a front-half vector comprising the nucleotide sequence of SEQ ID NO: 33 and a back-half vector comprising the nucleotide sequence of SEQ ID NO: 32; the vector system with a front-half vector comprising the nucleotide sequence of SEQ ID NO: 34 and a back-half vector comprising the nucleotide sequence of SEQ ID NO: 35; and/or the vector system with a front-half vector comprising the nucleotide sequence of SEQ ID NO: 34 and a back-half vector comprising the nucleotide sequence of SEQ ID NO: 44. 
     In some embodiments, any vector of the hybrid polynucleotide vector systems described in the disclosure may be administered by parenteral administration, such as intravenous, intramuscular, intraocular, intranasal, or (intra-)utricle injection, etc. The vector can be administered in vivo, in vitro or ex vivo. 
     In some embodiments, any vector of the hybrid polynucleotide vector systems described herein may be administered to the eye. In particular embodiments, a vector is administered to the eye of a subject by subretinal injection. In some embodiments, any vector of the hybrid polynucleotide vector systems described herein may be administered to the ear. 
     In some embodiments, any vector of the hybrid polynucleotide vector systems described herein the polynucleotide vector system is administered to the ear of a subject, e.g., by a round window injection or during cochlear implant surgery. 
     The methods of the disclosure can be used with humans and other animals. Animals contemplated within the scope of the disclosure include, for example, dogs, cats, rabbits, ferrets, guinea pigs, hamsters, pigs, monkeys or other primates, mice, gerbils, horses, mules, donkeys, burros, cattle, cows, pigs, sheep, and alligators. As used herein, the terms “patient” and “subject” are used interchangeably and are intended to include such human and non-human species, including human and non-human cells. Likewise, in vitro methods of the disclosure may also be performed on cells of one or more human or non-human, mammalian species, including human and non-human cells. 
     Components of Exemplary Dual AAV Vectors 
     Any of the dual polynucleotide vector systems of the disclosure may be used in conjunction with an AAV vector system known in the art. In treating some diseases, it may be preferable to administer the rAAV vector construct a single time, while in the management or treatment of other diseases or conditions, it may be desirable to provide two or more administrations of the vector constructs to the patient in one or more administration periods. In such circumstances, the AAV vector-based therapeutics may be provided successively in one or more daily, weekly, monthly, or less-frequent periods, as may be necessary to achieve treatment, or amelioration of one or more symptoms of the disease or disorder being treated. In some embodiments, the vector may be provided to one or both eyes by one or more administrations of an infectious adeno-associated viral particle, an rAAV virion, or a plurality of infectious rAAV particles in an amount and for a time sufficient to treat or ameliorate one or more symptoms of the disease or condition being treated. 
     In particular embodiments, the disclosure provides rAAV particles been derived from a number of different serotypes, including, for example, those selected from the group consisting of AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, and AAV10. In exemplary embodiments, particles derived from AAV2, AAV5 and AAV8 serotype vectors are utilized. In particular embodiments, particles having an AAV8(Y733F) or AAV2(tripleY-F) capsid are used. Accordingly, the disclosure provides recombinant AAV particles derived from, e.g., AAV8(Y733F) or AAV2(tripleY-F), that comprise overlap and hybrid polynucleotide vector systems. In some embodiments, the serotype of the AAV vector is not AAV6 or AAV2. 
     Additional exemplary capsids include AAV2, AAV6, and capsids derived from AAV2 and AAV6. Such capsids include AAV7m8, AAV-DJ, AAV2/2-MAX, AAVSHh10, AAVSHh10Y, AAV3b, AAVLK03, AAV8PB2, AAV1(E531K), AAV6(D532N), AAV6-3pmut, AAV2G9, AAV44.9, AAV44.9(E531D), AAVrh.8, AAVrh.8R, AAV9-PHP.B, and/or AAVAnc80. In some embodiments, the virus or virion is packaged in an AAV5, AAV7, AAV8, AAV9, AAV44.9, AAV44.9(E531D), AAV2(4pMut), AAVAnc80, AAVrh.8, AAVrh.8R, AAV9-PHP.B, AAVrh.10, or AAVrh.74 capsid. 
     The AAV-DJ capsid is described in Grimm et al.,  J. Virol.,  2008, 5887-5911 and Katada et al., (2019), Evaluation of AAV-DJ vector for retinal gene therapy,  PeerJ  7:e6317, each of which is herein incorporated by reference. The AAV7m8 capsid, which is closely related to AAV-DJ, is described in Dalkara, et al.  Sci Transl Med.  2013; 5(189):189ra76, herein incorporated by reference. The AAV2/2-MAX capsid is described in Reid, Ertel &amp; Lipinski, Improvement of Photoreceptor Targeting via Intravitreal Delivery in Mouse and Human Retina Using Combinatory rAAV2 Capsid Mutant Vectors,  Invest. Ophthalmol Vis Sci.  2017; 58:6429-6439, herein incorporated by reference. The AAV2/2-MAX capsid comprises five point mutations, Y272F, Y444F, Y500F, Y730F, T491V. The AAV1(E531K) capsid is described in Boye et al., Impact of Heparin Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors,  J. Virol.  90:4215-4231 (2016), herein incorporated by reference. The AAVSHh10 and AAV6(D532N) capsids, both derivatives of AAV6, are described in Klimczak et al., (2009) A Novel Adeno-Associated Viral Variant for Efficient and Selective Intravitreal Transduction of Rat Muller Cells,  PLoS ONE  4(10): e746, herein incorporated by reference. The AAV6-3pmut (also known as AAV6(TM6) and AAV6(Y705+Y731F+T492V)) capsid is described in Rosario et al., Microglia-specific targeting by novel capsid-modified AAV6 vectors,  Mol Ther Methods Clin Dev . (2016); 13(3):16026 and International Patent Publication No. 2016/126857, each of which are herein incorporated by reference. 
     Additional capsids suitable for use with the disclosed methods include the following: capsids comprising non-native amino acid substitutions at amino acid residues of a wild-type AAV2 capsid, wherein the non-native amino acid substitutions comprise one or more of Y272F, Y444F, T491V, Y500F, Y700F, Y704F and Y730F; capsids comprising non-native amino acid substitutions at amino acid residues of a wild-type AAV6 capsid, wherein the non-native amino acid substitutions comprise one or more of Y445F, Y705F, Y731F, T492V and S663V. In certain embodiments, the capsid comprises AAV2G9, a variant of AAV2. 
     In other embodiments, the capsid comprises a non-native amino acid substitution at amino acid residue 533 or 733 of a wild-type AAV8 capsid, wherein the non-native amino acid substitution is E533K, Y733F, or a combination thereof. The AAV8(Y733F) capsid is described in Doroudchi et al.,  Amer. Soc. of Gene  &amp;  Cell Ther.  19(7): 1220-29 (2011). In certain embodiments of the disclosed methods, the capsid comprises AAV8PB2, a variant of AAV8. 
     In other embodiments, the capsid comprises non-native amino acid substitutions of a wild-type AAV2 capsid comprising one or more of the following mutations:
         (a) Y444F;   (b) Y444F+Y500F+Y730F;   (c) Y272F+Y444F+Y500F+Y730F;   (d) Y444F+Y500F+Y730F+T491V; or   (e) Y272F+Y444F+Y500F+Y730F+T491V.       

     In other embodiments, the capsid comprises non-native amino acid substitutions of a wild-type AAV6 capsid, comprising one or more of the following mutations:
         (a) Y445F;   (b) Y705F+Y731F;   (c) T492V;   (d) Y705F+Y731F+T492V;   (e) S663V; or   (f) S663V+T492V.       

     Additional capsids suitable for use with the disclosed methods are described in International Patent Publication No. WO 2018/156654, published Aug. 30, 2018, herein incorporated by reference in its entirety. In particular embodiments, the rAAV particles of the disclosed invention comprise one of the following capsids: DGE-DF (also known as ‘V1V4 VR-V’), P2-V2, P2-V3, P2-V1 (also known as ME-B), and P2-V1(Y-F+T-V) (also known as ME-B(Y-F+T-V)). In still other embodiments, the rAAV particles may comprise a capsid selected from AAV6(3pMut) or AAV2(quadYF+T-V). In still other embodiments, the rAAV particles of the disclosed methods may comprise any of the capsid variants described in International Patent Publication No. WO 2018/156654. 
     In particular embodiments, disclosed herein are rAAV particles which may comprise a DGE-DF capsid, P2-V2 capsid, P2-V3 capsid, P2-V1 capsid (also known as ME-B), or P2-V1(Y-F+T-V) capsid for the enhanced transduction of said rAAV particles in retinal cells. In other embodiments, the disclosed rAAV particles may comprise a capsid selected from AAV2(Y444F), AAV2(Y444F+Y500F+Y730F), AAV2(Y272F+Y444F+Y500F+Y730F), AAV2(Y444F+Y500F+Y730F+T491V) and AAV2(Y272F+Y444F+Y500F+Y730F+T491V), AAV6(Y445F), AAV6(Y705F+Y731F), AAV6(Y705F+Y731F+T492V), AAV6(S663V), AAV6(T492V) or AAV6(S663V+T492V). 
     Exemplary inverted terminal repeat (ITR) sequences used in any AAV vector systems of the disclosure may comprise any AAV ITR. The ITRs used in an AAV vector can be the same or different. In particular embodiments, the ITR may be obtained from an AAV serotype 2 (AAV2), AAV serotype 5 (AAV5), AAV serotype 7 (AAV7), AAV serotype 8 (AAV8), AAV serotype 44.9 (AAV44.9), or a variant thereof, such as AAV serotype 44.9(E531D) and 44.9(Y73IF) (see PCT Application No. PCT/US2020/14838, filed Jan. 23, 2020, herein incorporated by reference). An AAV vector of the disclosure can comprise different AAV ITRs. In a non-limiting example, a vector may comprise an ITR of AAV2 and an ITR of AAV5. AAV ITR sequences are well known in the art (see, e.g., GenBank Accession Nos. AF043303.1; NC_001401.2; J01901.1; JN898962.1; K01624.1; and K01625.1). The AAV dual vector systems disclosed herein are able to efficiently express a therapeutic gene that is larger than what may ordinarily be packaged within a single AAV vector. 
     Accordingly, in some aspects the disclosure provides a virus or virion comprising any of the polynucleotides or vectors of the disclosure. In particular embodiments, the virus or virion is an AAV virus. Methods for preparing viruses and virions comprising a heterologous polynucleotide or vector are known in the art. In the case of AAV, cells can be co-infected or transfected with adenovirus or polynucleotide vectors comprising adenovirus genes suitable for AAV helper function. Examples of materials and methods are described, for example, in U.S. Pat. Nos. 8,137,962 and 6,967,018 (each of which is incorporated herein by reference). 
     In particular embodiments, the AAV serotype provides for one or more tyrosine to phenylalanine (Y-F) mutations on the capsid surface. In particular embodiments, the AAV is an AAV8 serotype having a tyrosine-to-phenylalanine (Y-F) mutation at position 733 (Y733F). The abilities to produce full-length MYO7A protein for second-generation hybrid and overlap vectors encapsidated in AAV5 and AAV8(Y733F) virions is shown in  FIGS.  36 A- 36 C and  45 B . AAV8(Y733F) virions outperformed AAV5 virions, as measured by Western blot (see  FIG.  45 B ). 
     In some embodiments, a triple-mutant AAV8 vector, which contains tyrosine-to-phenylalanine Tyr-Phe mutations at positions Y733F, Y500F, and Y730F, respectively, is used (see  FIG.  5   ). In other embodiments, a triple-mutant AAV8 vector, which contains tyrosine-to-phenylalanine Tyr-Phe mutations at positions Y447F, Y733F, and T494V (e.g. AAV8(Y447F+Y733F+T494F)) is used. 
     In exemplary embodiments, the rAAV particles of the disclosure may comprise a transgene, or heterologous nucleic acid, that is too large for delivery in standard AAV systems. Exemplary transgenes encode at least one diagnostic or therapeutic protein or polypeptide selected from the group consisting of a molecular marker, photosensitive opsins, including, without limitation, rhodopsin, melanopsin, cone opsins, channel rhodopsins, bacterial or archaea-associated opsins, an adrenergic agonist, an anti-apoptosis factor, an apoptosis inhibitor, a cytokine receptor, a cytokine, a cytotoxin, an erythropoietic agent, a glutamic acid decarboxylase, a glycoprotein, a growth factor, a growth factor receptor, a hormone, a hormone receptor, an interferon, an interleukin, an interleukin receptor, a kinase, a kinase inhibitor, a nerve growth factor, a netrin, a neuroactive peptide, a neuroactive peptide receptor, a neurogenic factor, a neurogenic factor receptor, a neurophilin, a neurotrophic factor, a neurotrophin, a neurotrophin receptor, an N-methyl-D-aspartate antagonist, a plexin, a protease, a protease inhibitor, a protein decarboxylase, a protein kinase, a protein kinase inhibitor, a proteolytic protein, a proteolytic protein inhibitor, a semaphorin, a semaphorin receptor, a serotonin transport protein, a serotonin uptake inhibitor, a serotonin receptor, a serpin, a serpin receptor, a tumor suppressor, and any combination thereof. 
     In some embodiments, the transgene is hMYO7A, which encodes a human myosin VIIa polypeptide. In particular embodiments, a hMYO7A polypeptide comprises the amino acid sequence shown in SEQ ID NO: 6 or SEQ ID NO: 8, or a functional fragment or a variant thereof. In particular embodiments, the hMYO7A polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO: 5 or SEQ ID NO: 7. 
     In some embodiments, the transgene is USH1C, CDH23, PCDH15 and USH1G, all of which are associated with Usher syndrome type I. In some embodiments, the transgene is USH2A or DFNB31, both of which are associated with Usher syndrome type II. In some embodiments, the transgene is ABCA4, CEP290, EYS, RP1, ALMS1, CDH23, PCDH15, DFNB2 or USHERIN. 
     In some embodiments, administration of any of the disclosed polynucleotide vectors to the eye of a subject in need thereof restores vision loss, partially or completely. The transgene may comprise a human MYO7A. These administrations may provide a partial or complete restoration of melanosome apical migration in retinal pigment epithelium (RPE) cells. 
     In some embodiments, the production of the therapeutic agent encoded by the transgene of any of the disclosed polynucleotide vector systems in cells of the eye (such as retinal cells or RPE cells) provides one or more of the following therapeutic endpoints: a) preserves one or more photoreceptor cells or one or more RPE cells, b) restores one or more rod- and/or cone-mediated functions, c) restores visual behavior in one or both eyes, or d) any combination thereof. In particular embodiments, production of the therapeutic agent in the disclosed methods preserves one or more PR cells, such as retinal ganglion cells, bipolar cells, Müller glial cells or astrocyte cells, or RPE cells. 
     In some embodiments, production of the therapeutic agent persists in the one or more photoreceptor cells or the one or more RPE cells substantially for a period of at least three months, at least six months, at least nine months, or at least a year or more, following an initial administration of any of the disclosed rAAV polynucleotide vector system into the one or both eyes of the mammal. 
     In some embodiments, administration of any of the disclosed polynucleotide vectors to the inner ear of a subject in need thereof restores hearing loss, partially or completely. In some embodiments, administration to the inner ear restores age-related hearing loss. The transgene may comprise a human MYO7A. These administrations may provide a partial or complete restoration of vestibular function in the inner ears. In some embodiments, any of the disclosed hybrid or overlap vectors may be administered to a vestibular hair cell, an inner ear hair cell, an outer ear hair cell, or a combination thereof. 
     In this manner, the polynucleotide vector systems and compositions thereof of the disclosure may be used to treat or ameliorate symptoms of USH1B (Usher Syndrome type 1B) in the eyes and/or inner ear of the subject. Likewise, administration of the vector systems and compositions of the disclosure may be used to treat or ameliorate symptoms of autosomal recessive isolated deafness (DFNB2), hearing loss, and/or vision loss. As an example, administration of the vector systems and compositions of the disclosure may be used to treat or ameliorate hearing loss associated with insufficiency of MYO7A protein expression (which may present in a USH1B patient). In some embodiments, administration of the vector systems and compositions of the disclosure may be used to treat or ameliorate age-related hearing loss presenting in carriers of a recessive defective MYO7A allele (i.e., USH1B carriers) or age-related hearing loss as the consequence of non-genetic deficiency or insufficiency in MYO7A expression. 
     As another example, administration of the vector systems and compositions of the disclosure may provide a restoration of melanosome migration in retinal pigment epithelium (RPE) cells. 
     In some embodiments, the disclosure provides rAAV nucleic acid vectors that include at least a first nucleic acid segment that encodes one or more diagnostic or therapeutic agents that alter, inhibit, reduce, prevent, eliminate, or impair the activity of one or more endogenous biological processes in a mammalian cell suitably transformed with the vector of interest. In certain embodiments, such diagnostic or therapeutic agents may include a molecule that selectively inhibits or reduces the effects of one or more metabolic processes, dysfunctions, disorders, or diseases. In certain embodiments, the defect may be caused by injury or trauma to the mammal for which treatment is desired. In other embodiments, the defect may be caused the over-expression of an endogenous biological compound, while in other embodiments still; the defect may be caused by the under-expression or even lack of one or more endogenous biological compounds. 
     Regulatory Elements of rAAV Vectors 
     Any of the vector systems of the disclosure may include regulatory elements that are functional in the intended host cell in which the vector is to be expressed. A person of ordinary skill in the art can select regulatory elements for use in appropriate host cells, for example, mammalian or human host cells. Regulatory elements include, for example, promoters, transcription termination sequences, translation termination sequences, enhancers, and polyadenylation elements. 
     Any of the vector systems of the disclosure may include a promoter sequence operably linked to a nucleotide sequence encoding a desired polypeptide. Promoters contemplated for use in the disclosure include, but are not limited to, cytomegalovirus (CMV) promoter, SV40 promoter, human myosin 7a gene-derived promoter, Rous sarcoma virus (RSV) promoter, chimeric CMV/chicken β-actin promoter (CBA) and the truncated form of CBA (smCBA) (see, e.g., Haire et al. 2006 and U.S. Pat. No. 8,298,818, each of which is incorporated herein by reference). Additional photoreceptor-specific, human rhodopsin kinase (hGRK1) promoter, a synapsin promoter, a glial fibrillary acidic protein (GFAP) promoter, rod specific IRBP promoter, VMD2 (vitelliform macular dystrophy/Best disease) promoter, a RPE-specific vitelliform macular dystrophy-2 [VMD2] promoter, and EF1-alpha promoter sequences are also contemplated to be useful in the practice of various aspects of the disclosure. Exemplary photoreceptor-cell-specific promoters include, but are not limited to, hGRK1, IRBP, rod opsin, NRL, GNAT2e-IRBP, L/M opsin, and cone arrestin promoters. 
     In particular embodiments, the promoter is a chimeric CMV-β-actin promoter. In particular embodiments, the promoter is a tissue-specific promoter that shows selective activity in one or a group of tissues but is less active or not active in other tissue. In particular embodiments, the promoter is a photoreceptor-specific promoter. In a further embodiment, the promoter is preferably a cone cell-specific promoter or a rod cell-specific promoter, or any combination thereof. In particular embodiments, the promoter is the promoter for human MYO7A gene. In a further embodiment, the promoter comprises a cone transducin a (TαC) gene-derived promoter. In particular embodiments, the promoter is a human GNAT2-derived promoter. Other promoters contemplated within the scope of the disclosure include, without limitation, a rhodopsin promoter (human or mouse), a cGMP-phosphodiesterase β-subunit promoter, a retinitis pigmentosa-specific promoter, an RPE cell-specific promoter [such as a vitelliform macular dystrophy-2 (VMD2) promoter (Best1) (Esumi et al., 2004)], or any combination thereof. 
     Promoters can be incorporated into a vector using standard techniques known to those of ordinary skill in the molecular biology and/or virology arts. Multiple copies of promoters, and/or multiple distinct promoters can be used in the vectors of the disclosure. In one such embodiment, a promoter may be positioned about the same distance from the transcription start site as it is from the transcription start site in its natural genetic environment, although some variation in this distance is permitted, of course, without a substantial decrease in promoter activity. In the practice of the disclosure, one or more transcription start site(s) are typically included within the disclosed vectors. 
     The vectors of the disclosure may further include one or more transcription termination sequences, one or more translation termination sequences, one or more signal peptide sequences, one or more internal ribosome entry sites (IRES), and/or one or more enhancer elements, or any combination thereof. Transcription termination regions can typically be obtained from the 3′-untranslated region of a eukaryotic or viral gene sequence. Transcription termination sequences can be positioned downstream of a coding sequence to provide for efficient termination. 
     Any of the disclosed polynucleotide vectors may also further include one or more post-transcriptional regulatory sequences or one or more polyadenylation signals, including, for example, but not limited to, a woodchuck hepatitis virus post-transcription regulatory element (WRPE), a polyadenylation signal sequence, or an intron/exon junctions/splicing signals, or any combination thereof. 
     Signal peptide sequences are amino-terminal peptidic sequences that encode information responsible for the location of an operably-linked polypeptide to one or more post-translational cellular destinations, including, for example, specific organelle compartments, or to the sites of protein synthesis and/or activity, and even to the extracellular environment. 
     Enhancers—cis-acting regulatory elements that increase gene transcription—may also be included in one of the disclosed AAV-based vector systems. A variety of enhancer elements are known to those of ordinary skill in the relevant arts, and include, without limitation, a CaMV 35S enhancer element, a cytomegalovirus (CMV) early promoter enhancer element, an SV40 enhancer element, as well as combinations and/or derivatives thereof. One or more nucleic acid sequences that direct or regulate polyadenylation of the mRNA encoded by a structural gene of interest, may also be optionally included in one or more of the vectors of the disclosure. 
     Host Cells and Methods for Transducing Cells 
     The disclosure provides host cells comprising vectors of the disclosed polynucleotide vector systems. In some embodiments, an isolated host cell comprising an overlap polynucleotide vector system is provided. In some embodiments, an isolated host cell comprising a hybrid polynucleotide vector system is provided. In particular embodiments, isolated host cells comprising second generation hybrid and isolated host cells comprising second generation overlap vectors are provided. 
     Examples of suitable host cells that comprise any of the disclosed dual vector systems include, but are not limited to, photoreceptor cells, cone cells, rod cells, retinal cells (e.g., ganglion cells, retinal pigment epithelium cells), or any combination thereof. Examples of retinal cells include retinal ganglion cells (RGCs), Muller cells, astrocytes, and bipolar cells. 
     Additional examples of suitable host cells are vestibular hair cells, inner ear hair cells, outer ear hair cells, or any combination thereof. 
     The disclosure also provides methods for expressing or transducing a selected polypeptide in a cell. In particular embodiments, the method comprises incorporating in the cell an AAV-based, dual vector system as disclosed herein, wherein the vector system includes a polynucleotide sequence that encodes a selected polypeptide and of interest, and expressing the polynucleotide sequences in the cell. 
     In certain embodiments, the selected polypeptide may be a polypeptide that is heterologous to the cell. In particular embodiments, the cell is a mammalian cell, and preferably, a human cell. In particular embodiments, the cell is a human photoreceptor cell, and preferably a human photoreceptor cone cell or a photoreceptor rod cell. In particular embodiments, the cell expresses a wild type, functional, and/or biologically-active hMYO7A polypeptide that is encoded by a nucleic acid segment present in a vector system as disclosed herein. In particular embodiments, the hMYO7A polypeptide is encoded by the nucleotide sequence shown in SEQ ID NO: 5 or SEQ ID NO: 7. 
     In particular embodiments, the cell is a photoreceptor cell. In particular embodiments, the cell is a cone cell; preferably, it is a human cone cell or a human rod cell. Such cells may express one or more nucleotide sequences provided in at least a first AAV-based, dual vector system of the disclosure. In particular embodiments, the cell expresses a wild-type, functional, and/or biologically active hMYO7A polypeptide that is encoded by a nucleic acid segment comprised within one or more of the AAV-based vector systems as disclosed herein. In particular embodiments, the hMYO7A polypeptide is encoded by the nucleotide sequence of SEQ ID NO: 5 or SEQ ID NO: 7. 
     Accordingly, in certain embodiments, the disclosure provides for methods for transducing or expressing a polynucleotide vector system in one or more photoreceptor cells or one or more RPE cells of a mammal (e.g., a human). In an overall and general sense, such a method includes administering (for example, directly administering subretinally) to one or both eyes of the mammal one or more of the rAAV particles disclosed herein, wherein the polynucleotide further comprises at least a first polynucleotide that comprises a PR- or an RPE-cell-specific promoter operably linked to at least a first heterologous nucleic acid segment that encodes a therapeutic agent (full-length polypeptide), for a time effective to produce the therapeutic agent in the one or more PR cells or RPE cells of the mammal. In certain embodiments, the therapeutic polypeptide is stably expressed in a photoreceptor cell, retinal pigment epithelium cell, retinal ganglion cell, bipolar cell, Müller glial cell or astrocyte cell, or combinations thereof. In certain embodiments, the therapeutic polypeptide is stably expressed in a vestibular hair cell, inner ear hair cell, or outer ear hair cell. 
     Methods of Treatment and Transduction 
     In some aspects, the disclosure provides methods for treating or ameliorating a disease or condition, such as an eye disease, in a human or animal using gene therapy and an AAV-based dual vector system of the disclosure. In particular embodiments, a method of the disclosure comprises administering a vector system of the disclosure that encodes a polypeptide that provides for treatment or amelioration of the disease or condition. In particular embodiments, the vectors of the disclosure are provided in an AAV virus or virion. The vector system can be administered in vivo or ex vivo. 
     In particular embodiments, a vector system of the disclosure is administered in a recombinant AAV particle by parenteral administration, such as intravitreal, subretinal, intravenous, intramuscular, intraocular, utricle, or intranasal injection. In some embodiments, vector systems are administered to, e.g., hair cells of the ear, by injection into the utricle, which is one of two sac-like otolith organs sensitive to gravity, as described in Lee et al., Hearing Research Vol. 394 (2020) 107882, incorporated by reference herein. Administration to, e.g., hair cells of the ear may be by a round window injection, or during cochlear implant surgery. In particular embodiments, a vector system of the disclosure is administered to the human or animal by intraocular, intravitreal or subretinal injection. 
     In some embodiments, the recombinant AAV particle of the disclosure is administered via subretinal injection in a titer of about 1×10 8  vg/ml, 5×10 8  vg/ml, 8×10 8  vg/ml, 1×10 9  vg/ml, 5×10 9  vg/ml, 1×10 10  vg/ml, 5×10 10  vg/ml, 1×10 11  vg/ml, 5×10 11  vg/ml, 1×10 12  vg/ml, 2×10 12  vg/ml, 3×10 12  vg/ml, 4×10 12  vg/ml, about 5×10 12  vg/ml, about 1×10 13  vg/ml, or about 5×10 13  vg/ml. In particular embodiments, the rAAV particle is administered in a titer of 5.0×10 8  vg or 8.0×10 8  vg. 
     In some embodiments, the subretinal injection is provided in a volume of about 200 μL, about 175 μL, about 160 μL, about 145 μL, about 130 μL, about 115 μL, about 100 μL, about 90 μL, about 80 μL, about 70 μL, about 60 μL, about 55 μL, about 50 μL, about 45 μL, about 35 μL, about 20 μL, about 10 μL, or about 5 μL. In particular embodiments, the injection is provided in a volume of about 50 μL. Dosage regimes and effective amounts to be administered can be determined by ordinarily skilled clinicians. Administration may be in the form of a single dose or multiple doses. General methods for performing gene therapy using polynucleotides, expression constructs, and vectors are known in the art (see, e.g.,  Gene Therapy: Principles and Applications  (1999); and U.S. Pat. Nos. 6,461,606; 6,204,251 and 6,106,826, each of which is specifically incorporated herein in its entirety by express reference thereto). 
     In particular embodiments, the disease, disorder or condition to be treated is Usher Syndrome. In some embodiments, the disease or disorder to be treated is autosomal recessive isolated deafness (DFNB2). In other embodiments, the disease, disorder or condition such as age-related macular degeneration (AMD), wet AMD, dry AMD, or geographic atrophy. In certain embodiments, the disease or disorder is retinitis pigmentosa or glaucoma. 
     The disclosed dual vector systems may be introduced into one or more selected mammalian cells using any one or more of the methods that are known to those of ordinary skill in the gene therapy and/or viral arts. Such methods include, without limitation, transfection, microinjection, electroporation, lipofection, cell fusion, and calcium phosphate precipitation, as well as biolistic methods. In particular embodiments, the vectors of the disclosure may be introduced in vivo, including, for example, by lipofection (for example, DNA transfection via liposomes prepared from one or more cationic lipids) (see, e.g., Felgner et al., 1987). Synthetic cationic lipids (LIPOFECTIN®, Invitrogen Corp., La Jolla, Calif., USA) may be used to prepare liposomes that will encapsulate the vectors to facilitate their introduction into one or more selected cells. A vector system of the disclosure can also be introduced in vivo as “naked” DNA using methods known to those of ordinary skill in the art. 
     In an overall and general sense, the disclosed methods include at least the step of administering to one or both eyes of the mammal in need thereof, one or more of the disclosed rAAV particles herein, in an amount and for a time sufficient to treat or ameliorate the one or more symptoms of the disease, the disorder, the dysfunction, the injury, the abnormal condition, or the trauma in the mammal. In some embodiments, the mammal is a human. In some embodiments, the human is a neonate, a newborn, an infant, or a juvenile. In the practice of the present disclosure, it is contemplated that suitable patients will include, for example, humans that have, are suspected of having, are at risk for developing, or have been diagnosed with one or more retinal disorders, diseases, or dystrophies, including, without limitation, retinal disorders, diseases, and dystrophies that are genetically linked, or inheritable. 
     In some aspects, the present disclosure provides methods of use of the particles, vectors, virions, expression systems, compositions, and host cells described herein in a method for treating or ameliorating the symptoms, or in the preparation of medicaments for, treating or ameliorating the symptoms of various deficiencies in an eye of a mammal, and in particular one or more deficiencies in human photoreceptors or RPE cells. Exemplary diseases and disorders of the eye (e.g., caused by one or more genetic deficiencies in a PR or RPE cell) for treatment or amelioration of symptoms include Retinitis pigmentosa, Leber Congenital Amaurosis (e.g., LCA10), Age Related Macular Degeneration (AMD), wet AMD, dry AMD, uveitis, Best disease, Stargardt disease, Usher Syndrome, Geographic Atrophy, Diabetic Retinopathy, Retinoschisis, Achromatopsia, Choroideremia, Bardet Biedl Syndrome, and glycogen storage diseases (ocular manifestation). 
     In some embodiments, administration of any of the disclosed vectors, virions, or compositions to a subject in need thereof provides a partial or complete restoration of melanosome migration in retinal pigment epithelium (RPE) cells. In exemplary embodiments, administration of any of the polynucleotide vector systems, virions, or compositions provides a partial or complete restoration of vision loss. 
     In some aspects, the present disclosure provides methods of use of the particles, vectors, virions, expression systems, compositions, and host cells described herein in a method for treating or ameliorating the symptoms, or in the preparation of medicaments for, treating or ameliorating the symptoms of various deficiencies in an ear of a mammal, and in particular one or more deficiencies in hair cells of the auditory and hair cells of the vestibular systems. In exemplary embodiments, the subject in need thereof suffers from a disease or disorder selected from Usher syndrome or autosomal recessive isolated deafness (DFNB2). In some embodiments, the subject suffers from Usher Syndrome type 1B, 1D, 1F, or 2A. 
     In some embodiments, the subject suffers from a disease or condition of the eye, and/or a disease or disorder of the ear, selected from Stargardt Disease; LCA10; Retinitis Pigmentosa, Alstrom syndrome; Usher Syndrome type 1B, 1D, 1F, or 2A; Duchenne muscular dystrophy; Cystic fibrosis; Glycogen storage disease III; non-syndromic deafness; Hemophilia A, or a dysferlinopathy. 
     Such methods may involve intravitreal or subretinal administration to one or both eyes of a subject in need thereof, one or more of the disclosed particles vectors, virions, host cells, or compositions, in an amount and for a time sufficient to treat or ameliorate the symptoms of such a deficiency in the affected mammal. The methods may also encompass prophylactic treatment of animals suspected of having such conditions, or administration of such compositions to those animals at risk for developing such conditions either following diagnosis, or prior to the onset of symptoms. 
     Pharmaceutical Compositions and Kits 
     Pharmaceutical dosage forms suitable for injection or infusion can include sterile aqueous solutions or dispersions or sterile powders comprising the active ingredient, which are adapted for the extemporaneous preparation of sterile injectable or infusible solutions or dispersions, optionally encapsulated in liposomes. The ultimate dosage form should be sterile, fluid and stable under the conditions of manufacture and storage. The liquid carrier or vehicle can be a solvent or liquid dispersion medium comprising, for example, water, ethanol, a polyol (e.g., glycerol, propylene glycol, liquid polyethylene glycols, and the like), vegetable oils, nontoxic glyceryl esters, and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the formation of liposomes, by the maintenance of the required particle size in the case of dispersions or by the use of surfactants. Optionally, the prevention of the action of microorganisms can be brought about by various other antibacterial and antifungal agents, e.g., parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, e.g., sugars, buffers or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the inclusion of agents that delay absorption, e.g., aluminum monostearate and gelatin. 
     The disclosure also provides pharmaceutical compositions comprising a vector system of the disclosure in combination with a pharmaceutically acceptable carrier. Pharmaceutical compositions adapted for topical or parenteral administration, comprising an amount of a compound constitute a preferred embodiment of the disclosure. The dose administered to a patient, particularly a human, in the context of the disclosure should be sufficient to achieve a therapeutic response in the patient over a reasonable timeframe, without lethal toxicity, and preferably causing no more than an acceptable level of side effects or morbidity. One skilled in the art will recognize that dosage will depend upon a variety of factors including the condition (health) of the subject, the body weight of the subject, kind of concurrent treatment, if any, frequency of treatment, therapeutic ratio, as well as the severity and stage of the pathological condition. 
     The disclosure also provides kits comprising a vector system of the disclosure in one or more containers. Kits of the disclosure can optionally include pharmaceutically acceptable carriers and/or diluents. In particular embodiments, a kit of the disclosure includes one or more other components, adjuncts, or adjuvants as described herein. In particular embodiments, a kit of the disclosure includes instructions or packaging materials that describe how to administer a vector system contained within the kit to a selected mammalian recipient. 
     Containers of the disclosed kits may be of any suitable material, e.g., glass, plastic, metal, etc., and of any suitable size, shape, or configuration. In particular embodiments, a vector system of the disclosure is provided in the kit as a solid. In another embodiment, a vector system of the disclosure is provided in the kit as a liquid or solution. In certain embodiments, the kits may include one or more ampoules or syringes that contain a vector system of the disclosure in a suitable liquid or solution form. 
     Further contemplated herein are kits containing a pre-mixture of any of the disclosed dual vectors (front half vector and back half vector). These pre-mixtures may be in a single container and/or a single drug product in a suitable liquid or solution form. 
     The disclosure also provides for the use of the buffers and compositions disclosed herein in the manufacture of a medicament for treating, preventing or ameliorating the symptoms of a disease, disorder, dysfunction, injury or trauma, including, but not limited to, the treatment, prevention, and/or prophylaxis of a disease, disorder or dysfunction, and/or the amelioration of one or more symptoms of such a disease, disorder or dysfunction. 
     The amount of AAV compositions and time of administration of such compositions will be within the purview of the skilled artisan having benefit of the present teachings. The administration of therapeutically-effective amounts of the disclosed compositions may be achieved by a single administration, such as for example, a single injection of sufficient numbers of infectious particles to provide therapeutic benefit to the patient undergoing such treatment. Alternatively, in some circumstances, it may be desirable to provide multiple, or successive administrations of the AAV vector compositions, either over a relatively short, or over a relatively prolonged period, as may be determined by the medical practitioner overseeing the administration of such compositions. 
     For example, the number of infectious particles administered to a mammal may be approximately 10 7 , 10 8 , 10 9 , 10 10 , 10 11 , 10 12 , 10 13 , or even higher, infectious particles/mL, given either as a single dose (or divided into two or more administrations, etc.) as may be required to achieve therapy of the particular disease or disorder being treated. In fact, in certain embodiments, it may be desirable to administer two or more different rAAV particle- or vector-based compositions, either alone, or in combination with one or more other diagnostic agents, drugs, bioactives, or such like, to achieve the desired effects of a particular regimen or therapy. In most rAAV-vectored, gene therapy-based regimens, the inventors contemplate that lower titers of infectious particles will be required when practicing the disclosed methods of pre-treating and co-administering AAV capsids with HA. 
     To express a therapeutic agent in accordance with the present disclosure one may prepare a rAAV particle that comprises a therapeutic agent-encoding nucleic acid segment under the control of one or more promoters. To bring a sequence “under the control of” a promoter, one positions the 5′ end of the transcription initiation site of the transcriptional reading frame generally between about 1 and about 50 nucleotides “downstream” of (for example, 3′ of) the chosen promoter. The “upstream” promoter stimulates transcription of the DNA and promotes expression of the encoded polypeptide. This is the meaning of “recombinant expression” in this context. In some embodiments, recombinant vector constructs are those that include a capsid-protein modified rAAV vector that contains an RPE cell- or a photoreceptor cell-specific promoter, operably linked to at least one nucleic acid segment encoding one or more diagnostic, and/or therapeutic agents. 
     When the use of such vectors is contemplated for introduction of one or more exogenous proteins, polypeptides, peptides, ribozymes, and/or antisense oligonucleotides, to a particular cell transfected with the vector, one may employ the rAAV particles disclosed herein to deliver one or more exogenous polynucleotides to a selected host cell, e.g., to one or more selected cells within the mammalian eye. 
     In some embodiments, the number of viral particles administered to a subject may be on the order ranging from 10 6  to 10 14  particles/ml or 10 3  to 10 15  particles/ml, or any values therebetween for either range, such as for example, about 10 6 , 10 7 , 10 8 , 10 9 , 10 10 , 10 11 , 10 12 , 10 13 , or 10 14  particles/ml. In one embodiment, viral particles of higher than 10 13  particles/ml may be administered. In some embodiments, the number of viral particles administered to a subject may be on the order ranging from 10 6  to 10 14  vector genomes (vgs)/ml or 10 3  to 10 15  vgs/ml, or any values therebetween for either range, such as for example, about 10 6 , 10 7 , 10 8 , 10 9 , 10 10 , 10 11 , 10 12 , 10 13 , or 10 14  vgs/ml. In one embodiment, viral particles of higher than 10 13  vgs/ml are administered. The viral particles can be administered as a single dose, or divided into two or more administrations as may be required to achieve therapy of the particular disease or disorder being treated. In some embodiments, doses of 0.0001 ml to 10 ml, e.g., 0.001 ml, 0.01 ml, 0.1 ml, 1 ml, 2 ml, 5 ml or 10 ml, are delivered to a subject. 
     In some embodiments, the disclosure provides formulations of one or more viral-based compositions disclosed herein in pharmaceutically acceptable solutions for administration to a cell or an animal, either alone or in combination with one or more other modalities of therapy, and in particular, for therapy of human cells, tissues, and diseases affecting man. 
     If desired, rAAV particles described herein may be administered in combination with other agents as well, such as, e.g., proteins or polypeptides or various pharmaceutically-active agents, including one or more systemic or topical administrations of therapeutic polypeptides, biologically active fragments, or variants thereof. In fact, there is virtually no limit to other components that may also be included, given that the additional agents do not cause a significant adverse effect upon contact with the target cells or host tissues. The rAAV particles may thus be delivered along with various other agents as required in the particular instance. Such compositions may be purified from host cells or other biological sources, or alternatively may be chemically synthesized as described herein. 
     Formulation of pharmaceutically-acceptable buffer, excipients and carrier solutions is well known to those of skill in the art, as is the development of suitable dosing and treatment regimens for using the particular compositions described herein in a variety of treatment regimens, including e.g., oral, parenteral, intraocular (e.g., subretinal or intravitreal), intravenous, intranasal, intra-articular, intra-utricle, intracochlear and intramuscular administration and formulation. 
     Typically, these formulations may contain at least about 0.1% of the therapeutic agent (e.g., rAAV particle) or more, although the percentage of the active ingredient(s) may, of course, be varied and may conveniently be between about 1 or 2% and about 70% or 80% or more of the weight or volume of the total formulation. Naturally, the amount of therapeutic agent(s) in each therapeutically-useful composition may be prepared is such a way that a suitable dosage will be obtained in any given unit dose of the compound. Factors such as solubility, bioavailability, biological half-life, route of administration, product shelf life, as well as other pharmacological considerations will be contemplated by one skilled in the art of preparing such pharmaceutical formulations, and as such, a variety of dosages and treatment regimens may be desirable. 
     The term “excipient” refers to a diluent, adjuvant, carrier, or vehicle with which the rAAV particle is administered. Such pharmaceutical excipients can be sterile liquids, such as water and oils, including those of petroleum oil such as mineral oil, vegetable oil such as peanut oil, soybean oil, and sesame oil, animal oil, or oil of synthetic origin. Saline solutions and aqueous dextrose and glycerol solutions can also be employed as liquid carriers. Exemplary excipients and vehicles include, but are not limited to, HA, BSS, artificial CSF, PBS, Ringer&#39;s lactate solution, TMN200 solution, polysorbate 20, and poloxamer 100. 
     The amount of rAAV particle compositions and time of administration of such compositions will be within the purview of the skilled artisan having benefit of the present teachings. It is likely, however, that the administration of therapeutically-effective amounts of the disclosed compositions may be achieved by a single administration, such as for example, a single injection of sufficient numbers of viral particles to provide therapeutic benefit to the patient undergoing such treatment. Alternatively, in some circumstances, it may be desirable to provide multiple, or successive administrations of the compositions, either over a relatively short, or a relatively prolonged period of time, as may be determined by the medical practitioner overseeing the administration of such compositions. 
     Exemplary compositions may include rAAV particles or nucleic acid vectors either alone, or in combination with one or more additional active ingredients, which may be obtained from natural or recombinant sources or chemically synthesized. 
     Methods of Manufacturing rAAV Particles 
     Recombinant adeno-associated virus (rAAV) vectors have been used successfully for in vivo gene transfer in numerous pre-clinical animal models of human disease, and have been used successfully for long-term expression of a wide variety of therapeutic genes (Daya and Berns, 2008; Niemeyer et al., 2009; Owen et al., 2002; Keen-Rhinehart et al., 2005; Scallan et al., 2003; Song et al., 2004). AAV vectors have also generated long-term clinical benefit in humans when targeted to immune-privileged sites, for example, ocular delivery for Leber congenital amaurosis (Bainbridge et al., 2008; Maguire et al., 2008; Cideciyan et al., 2008). A major advantage of this vector is its comparatively low immune profile, eliciting only limited inflammatory responses and, in some cases, even directing immune tolerance to transgene products (LoDuca et al., 2009). Nonetheless, the therapeutic efficiency, when targeted to non-immune privileged organs, has been limited in humans due to antibody and CD8 +  T cell responses against the viral capsid, while in animal models, adaptive responses to the transgene product have also been reported (Manno et al., 2006; Mingozzi et al., 2007; Muruve et al., 2008; Vandenberghe and Wilson, 2007; Mingozzi and High, 2007). These results suggested that immune responses remain a concern for AAV vector-mediated gene transfer. 
     Adeno-associated virus (AAV) is considered the optimal vector for ocular gene therapy due to its efficiency, persistence and low immunogenicity (Daya and Berns, 2008). Identifying vectors capable of transducing PRs via the vitreous has historically relied on identifying which serotypes have native tropism for this cell type following local delivery. Several serotypes have been used to successfully target transgene to PRs following subretinal injection (including, e.g., AAV2, AAV5 and AAV8) with all three demonstrating efficacy in experiments performed across multiple mammalian species (e.g., mouse, rat, dog, pig and non-human primate) (Ali et al., 1996; Auricchio et al., 2001; Weber et al., 2003; Yang et al., 2002; Acland et al., 2001; Vandenberghe et al., 2011; Bennett et al., 1999; Allocca et al., 2007; Petersen-Jones et al., 2009; Lotery et al., 2003; Boye et al., 2012; Stieger et al., 2008; Mussolino et al., 2011; Vandenberghe et al., 2011). 
     Studies comparing their relative efficiency following subretinal delivery in the rodent show that both AAV5 and AAV8 transduce PRs more efficiently than AAV2, with AAV8 being the most efficient (Yang et al., 2002; Allocca et al., 2007; Rabinowitz et al., 2002; Boye et al., 2011; Pang et al., 2011). It was previously shown that AAV2 and AAV8 vectors containing point mutations of surface-exposed tyrosine residues (tyrosine to phenylalanine, Y-F) display increased transgene expression in a variety of retinal cell types relative to unmodified vectors following both subretinal and intravitreal injection (Petrs-Silva et al., 2009; Petrs-Silva et al., 2011). Of the vectors initially tested by those authors, an AAV2 triple mutant (designated “triple Y-F”) exhibited the highest transduction efficiency following intravitreal injection, whereas an AAV2 quadruple mutant (“quad Y-F”) exhibited the novel property of enhanced transduction of outer retina (Petrs-Silva et al., 2011). 
     Further improvements in transduction efficiency have been achieved via directed mutagenesis of surface-exposed threonine (T) or serine (S) residues to non-native amino acids at one of more of those amino acids. Both Y-F and T-V/T-A mutations have been shown to increase efficiency by decreasing phosphorylation of capsid and subsequent ubiquitination as part of the proteosomal degradation pathway (Zhong et al., 2008; Aslanidi et al., In Press; Gabriel et al., 2013). It has been found that the transduction profile of intravitreally-delivered AAV is heavily dependent upon the injection procedure itself. Due to the small size of the mouse eye, it is not uncommon for trans-scleral, intravitreal injections to result in damage to the retina that might allow delivery of some vector directly to the subretinal space. 
     Exemplary rAAV nucleic acid vectors useful according to the disclosure include single-stranded (ss) or self-complementary (sc) AAV nucleic acid vectors, such as single-stranded or self-complementary recombinant viral genomes. 
     Methods of producing rAAV particles and nucleic acid vectors are also known in the art and commercially available (see, e.g., Zolotukhin et al., Production and purification of serotype 1, 2, and 5 recombinant adeno-associated viral vectors,  Methods  28 (2002) 158-167; and U.S. Patent Publication Nos. US 2007/0015238 and US 2012/0322861, which are incorporated herein by reference; and plasmids and kits available from ATCC and Cell Biolabs, Inc.). For example, a plasmid containing the nucleic acid vector sequence may be combined with one or more helper plasmids, e.g., that contain a rep gene (e.g., encoding Rep78, Rep68, Rep52 and Rep40) and a cap gene (encoding VP1, VP2, and VP3, including a modified VP3 region as described herein), and transfected into a producer cell line such that the rAAV particle can be packaged and subsequently purified. 
     In some embodiments, the one or more helper plasmids includes a first helper plasmid comprising a rep gene and a cap gene and a second helper plasmid comprising a Ela gene, a E1b gene, a E4 gene, a E2a gene, and a VA gene. In some embodiments, the rep gene is a rep gene derived from AAV2 and the cap gene is derived from AAV2 and includes modifications to the gene in order to produce a modified capsid protein described herein. Helper plasmids, and methods of making such plasmids, are known in the art and commercially available (see, e.g., pDM, pDG, pDPlrs, pDP2rs, pDP3rs, pDP4rs, pDP5rs, pDP6rs, pDG(R484E/R585E), and pDP8.ape plasmids from PlasmidFactory, Bielefeld, Germany; other products and services available from Vector Biolabs, Philadelphia, Pa.; Cellbiolabs, San Diego, Calif.; Agilent Technologies, Santa Clara, Calif.; and Addgene, Cambridge, Mass.; pxx6; Grimm et al. (1998), Novel Tools for Production and Purification of Recombinant Adenoassociated Virus Vectors,  Human Gene Therapy, Vol.  9, 2745-2760; Kern, A. et al. (2003), Identification of a Heparin-Binding Motif on Adeno-Associated Virus Type 2 Capsids, Journal of Virology, Vol. 77, 11072-11081; Grimm et al. (2003), Helper Virus-Free, Optically Controllable, and Two-Plasmid-Based Production of Adeno-associated Virus Vectors of Serotypes 1 to 6, Molecular Therapy, Vol. 7, 839-850; Kronenberg et al. (2005), A Conformational Change in the Adeno-Associated Virus Type 2 Capsid Leads to the Exposure of Hidden VP1 N Termini, Journal of Virology, Vol. 79, 5296-5303; and Moullier, P. and Snyder, R. O. (2008), International efforts for recombinant adeno-associated viral vector reference standards, Molecular Therapy, Vol. 16, 1185-1188). 
     An exemplary, non-limiting, rAAV particle production method is described next. One or more helper plasmids are produced or obtained, which comprise rep and cap ORFs for the desired AAV serotype and the adenoviral VA, E2A (DBP), and E4 genes under the transcriptional control of their native promoters. The cap ORF may also comprise one or more modifications to produce a modified capsid protein as described herein. HEK293 cells (available from ATCC®) are transfected via CaPO4-mediated transfection, lipids or polymeric molecules such as Polyethylenimine (PEI) with the helper plasmid(s) and a plasmid containing a nucleic acid vector described herein. The HEK293 cells are then incubated for at least 60 hours to allow for rAAV particle production. Alternatively, in another example Sf9-based producer stable cell lines are infected with a single recombinant baculovirus containing the nucleic acid vector. As a further alternative, in another example HEK293 or BHK cell lines are infected with a HSV containing the nucleic acid vector and optionally one or more helper HSVs containing rep and cap ORFs as described herein and the adenoviral VA, E2A (DBP), and E4 genes under the transcriptional control of their native promoters. The HEK293, BHK, or Sf9 cells are then incubated for at least 60 hours to allow for rAAV particle production. The rAAV particles can then be purified using any method known the art or described herein, e.g., by iodixanol step gradient, CsCl gradient, chromatography, or polyethylene glycol (PEG) precipitation. 
     Exemplary Definitions 
     In accordance with the disclosure, polynucleotides, nucleic acid segments, nucleic acid sequences, and the like, include, but are not limited to, DNAs (including, but not limited to, genomic and/or extragenomic DNAs), genes, peptide nucleic acids (PNAs) RNAs (including, but not limited to, rRNAs, mRNAs, and/or tRNAs), nucleosides, as well as one or more nucleic acid segments obtained from natural sources, chemically synthesized, genetically modified, or otherwise prepared or synthesized in whole or in part by the hand of man. 
     Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and compositions similar or equivalent to those described herein can be used in the practice or testing of the disclosure, the preferred methods and compositions are described herein. For purposes of the disclosure, the following terms are defined below: 
     As used herein, the terms “nucleic acid” and “polynucleotide sequence” refer to a deoxyribonucleotide or ribonucleotide polymer in either single- or double-stranded form, and unless otherwise limited, encompass known analogs of natural nucleotides that can function in a similar manner as naturally occurring nucleotides. The polynucleotide sequences include both full-length sequences, as well as shorter sequences derived from the full-length sequences. It is understood that a particular polynucleotide sequence includes the degenerate codons of the native sequence or sequences that may be introduced to provide codon preference in a specific host cell. The polynucleotide sequences falling within the scope of the disclosure further include sequences that specifically hybridize with the sequences coding for a peptide of the disclosure. The polynucleotide includes both the sense and antisense strands, either as individual strands or in the duplex. 
     Fragments and variants of a polynucleotide of the disclosure can be generated as described herein and tested for the presence of function using standard techniques known in the art. Thus, an ordinarily skilled artisan can readily prepare and test fragments and variants of a polynucleotide or polypeptide of the disclosure and determine whether the fragment or variant retains functional activity that is the same or similar to a full-length or a non-variant polynucleotide or polypeptide, such as a myosin VIIa polynucleotide or polypeptide. 
     Also within the scope of the disclosure are polynucleotides that have the same, or substantially the same, nucleotide sequence of a polynucleotide exemplary herein, except for the presence of one or more nucleotide substitutions, additions, or deletions within the sequence of the polynucleotide, so long as these variant polynucleotides retain substantially the same relevant functional activity as the polynucleotides exemplary herein (for example, they encode a protein having the same amino acid sequence or the same functional activity as one of the polynucleotides specifically exemplary herein). Thus, the polynucleotides disclosed herein should also be understood to include variants and fragments thereof. 
     As one of ordinary skill in the molecular biological arts can readily appreciate, there can be a number of variant sequences of a gene or polynucleotide found in nature, in addition to those variants that may be artificially prepared or synthesized by an ordinary-skilled artisan in a laboratory environment. The polynucleotides of the disclosure encompasses those specifically exemplary herein, as well as any natural variants thereof, as well as any variants which can be created artificially, so long as those variants retain the desired biological activity. 
     Also within the scope of the disclosure are polynucleotides which have the same nucleotide sequences of a polynucleotide exemplary herein except for nucleotide substitutions, additions, or deletions within the sequence of the polynucleotide, as long as these variant polynucleotides retain substantially the same relevant biological activity as the polynucleotides specifically exemplary herein. Thus, the polynucleotides disclosed herein should be understood to include variants and fragments, as discussed above, of the specifically exemplary sequences. 
     Polynucleotides described herein can also be defined in terms of more particular identity and/or similarity ranges with those exemplary herein. The sequence identity will typically be greater than 60%, preferably greater than 75%, more preferably greater than 80%, even more preferably greater than 90%, and can be greater than 95%. The identity and/or similarity of a sequence can be 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99% or greater as compared to a sequence exemplary herein. 
     Unless otherwise specified, as used herein percent sequence identity and/or similarity of two sequences can be determined using the algorithm of Karlin and Altschul (1990), modified as in Karlin and Altschul (1993). Such an algorithm is incorporated into the NBLAST and XBLAST programs of Altschul et al. (1990). BLAST searches can be performed with the NBLAST program, score=100, word-length=12, to obtain sequences with the desired percent sequence identity. To obtain gapped alignments for comparison purposes, Gapped BLAST can be used as described (Altschul et al., 1997). When utilizing BLAST and Gapped BLAST programs, the default parameters of the respective programs (NBLAST and XBLAST) can be used in accordance with published methods. 
     The disclosure also contemplates those polynucleotide molecules having sequences that are sufficiently homologous with the polynucleotide sequences of the disclosure to permit hybridization with that sequence under standard stringent conditions and standard methods (Maniatis et al., 1982). As used herein, “stringent” conditions for hybridization refers to conditions wherein hybridization is typically carried out overnight at 20-25 degrees Celsius below the melting temperature (Tm) of the DNA hybrid in 6×SSPE, 5×Denhardt&#39;s solution, and 0.1% SDS, containing 0.1 mg/mL of a suitable non-specific denatured DNA. 
     The term “effective amount,” as used herein, refers to an amount that is capable of treating or ameliorating a disease or condition or otherwise capable of producing an intended therapeutic effect. 
     The term “operably linked,” as used herein, refers to that the nucleic acid sequences being linked are typically contiguous, or substantially contiguous, and, where necessary to join two protein coding regions, contiguous and in reading frame. However, since enhancers generally function when separated from the promoter by several kilobases and intronic sequences may be of variable lengths, some polynucleotide elements may be operably linked but not contiguous. 
     The term “promoter,” as used herein, refers to a region or regions of a nucleic acid sequence that regulates transcription. Exemplary promoters provided herein include, but are not limited to, a CMV promoter, an EF-1 alpha promoter, a cone arrestin promoter, a chimeric CMV β actin promoter (CBA), a truncated chimeric CMV β actin (smCBA) promoter, a human myosin 7a gene-derived promoter, a TαC gene-derived promoter, a rhodopsin promoter, a cGMP-phosphodiesterase β-subunit promoter, human or mouse rhodopsin promoter, a hGRK1 promoter, a synapsin promoter, a glial fibrillary acidic protein (GFAP) promoter, a rod specific IRBP promoter, a VMD2 promoter. 
     The term “regulatory element,” as used herein, refers to a region or regions of a nucleic acid sequence that regulates transcription. Exemplary regulatory elements include, but are not limited to, enhancers, post-transcriptional elements, transcriptional control sequences, and such like. 
     The term “substantially corresponds to,” “substantially homologous,” or “substantial identity,” as used herein, denote a characteristic of a nucleic acid or an amino acid sequence, wherein a selected nucleic acid or amino acid sequence has at least about 70 or about 75 percent sequence identity as compared to a selected reference nucleic acid or amino acid sequence. More typically, the selected sequence and the reference sequence will have at least about 76, 77, 78, 79, 80, 81, 82, 83, 84 or even 85 percent sequence identity, and more preferably, at least about 86, 87, 88, 89, 90, 91, 92, 93, 94, or 95 percent sequence identity. More preferably still, highly homologous sequences often share greater than at least about 96, 97, 98, or 99 percent sequence identity between the selected sequence and the reference sequence to which it was compared. 
     The percentage of sequence identity may be calculated over the entire length of the sequences to be compared, or may be calculated by excluding small deletions or additions which total less than about 25 percent or so of the chosen reference sequence. The reference sequence may be a subset of a larger sequence, such as a portion of a gene or flanking sequence, or a repetitive portion of a chromosome. However, in the case of sequence homology of two or more polynucleotide sequences, the reference sequence will typically comprise at least about 18-25 nucleotides, more typically at least about 26 to 35 nucleotides, and even more typically at least about 40, 50, 60, 70, 80, 90, or even 100 or so nucleotides. 
     When highly-homologous fragments are desired, the extent of percent identity between the two sequences will be at least about 80%, preferably at least about 85%, and more preferably about 90% or 95% or higher, as readily determined by one or more of the sequence comparison algorithms well-known to those of ordinary skill in the art, such as e.g., the FASTA program analysis described by Pearson and Lipman (1988). 
     The term “subject,” as used herein, describes an organism, including mammals such as primates, to which treatment with the compositions according to the disclosure can be provided. Mammalian species that can benefit from the disclosed methods of treatment include, but are not limited to, humans, non-human primates such as apes; chimpanzees; monkeys, and orangutans, domesticated animals, including dogs and cats, as well as livestock such as horses, cattle, pigs, sheep, and goats, or other mammalian species including, without limitation, mice, rats, guinea pigs, rabbits, hamsters, and the like. 
     The term “treatment” or any grammatical variation thereof (e.g., treat, treating, and treatment, etc.), as used herein, includes but is not limited to, alleviating a symptom of a disease or condition; and/or reducing, suppressing, inhibiting, lessening, ameliorating or affecting the progression, severity, and/or scope of a disease or condition. 
     The term “vector,” as used herein, refers to a nucleic acid molecule (typically one containing DNA) that is capable of replication in a suitable host cell, or one to which another nucleic acid segment can be operatively linked so as to facilitate replication of the operably-linked nucleic acid segment. Exemplary vectors include, without limitation, plasmids, cosmids, viruses and the like. 
     As used herein, the term “variant” refers to a molecule (e.g., a polynucleotide) having characteristics that deviate from what occurs in nature, e.g., a “variant” is at least about 80% identical, at least about 90% identical, at least about 95% identical, at least about 96% identical, at least about 97% identical, at least about 98% identical, at least about 99% identical, at least about 99.5% identical, or at least about 99.9% identical to the wild type polynucleotide. Variants of a protein molecule, e.g. a capsid, may contain modifications to the amino acid sequence (e.g., having 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 10-15, or 15-20 amino acid substitutions) relative to the wild type protein sequence, which arise from point mutations installed into the nucleic acid sequence encoding the capsid protein. These modifications include chemical modifications as well as truncations. 
     Variants of a nucleic acid molecule, e.g. a polynucleotide vector system, may contain modifications to the sequence (e.g., having 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 10-15, or 15-20 nucleotide substitutions) relative to the wild type nucleic acid sequence. These modifications may comprise truncations at a 5′ terminus or a 3′ terminus. 
     Examples 
     The following examples are included to demonstrate preferred embodiments of the disclosure. It should be appreciated by those of skill in the art that the techniques disclosed in the examples that follow represent techniques discovered by the inventor to function well in the practice of the disclosure, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the disclosure. 
     EXAMPLE 1 shows an exemplary Overlap Dual Vector System, which has a hMYO7A coding overlap. In an exemplary overlap system ( FIG.  2   ), the overlapping DNA sequence shared by both vector A and vector B consists of a 1350-bp coding region for the human MYO7A gene. This is the simplest system of the disclosure, and appears to be highly efficient in terms of full-length gene reconstitution, and MYO7A expression. Advantageously, each vector is of standard AAV packaging size, and as such, each packages DNA with a high degree of efficiency, and is readily adaptable to conventional GMP standards. Such vectors are also readily characterized to permit requisite regulatory approval prior to use in humans. 
     EXAMPLE 2 shows an exemplary hybrid dual-AAV vector system, which utilizes hMYO7A intron 23 splicing. In an exemplary system ( FIG.  3   ) the overlapping DNA sequence is composed of the native intron 23 of human MYO7A. Vector A contains the coding sequence corresponding to the amino-terminal portion of the hMYO7A cDNA relative to intron 23 (hMYO7ANT) and the native splice-donor site, followed by a 250 bp fragment of intron 23 of hMYO7A (minus the native acceptor site). Vector B contains the carboxyl-terminal portion of the hMYO7A cDNA relative to intron 23 (hMYO7ACT), and a 250 bp fragment of intron 23 of MYO7A (minus the native splice-donor site), followed by the native splice-acceptor site. Upon co-delivery to suitable mammalian host cells, the DNA of vectors A and B recombine to form a reconstituted full-length gene cassette. The resulting RNA transcript will then ‘splice out’ the native intron. Alternatively, recombination and formation of the gene cassette can occur via the AAV TRs. In this case, the RNA transcript will ‘splice out’ the native intron23-TR-intron23 motif. In both cases, however, the resulting mRNA is that of the reconstituted full-length hMYO7A gene sequence. 
     EXAMPLE 3 shows in vitro performance of an exemplary overlap vector system, which contains the hMYO7A coding overlap. HEK293 cells were infected simultaneously with vector A and vector B of an overlap dual vector system ( FIG.  2   ) at a ratio of 10000:1 vg/cell for each vector. The AAV vectors were packaged in AAV2 virions that contain three Y-F mutations in the capsid protein (see, e.g., Zhong et al., 2008). As a positive control, cells were transfected with plasmid containing full-length hMYO7A under the control of smCBA. Protein was recovered from cells at 3-, 4-, 5-, 6- and 7-days post-infection, and an antibody directed against MYO7A was used to assay for its presence in the infected cells via immunoblotting. The results are shown in  FIG.  6 A  and  FIG.  6 B . An area of interest from inside  FIG.  6 A  is magnified, and presented at higher contrast in  FIG.  6 B . Starting at 3-days post-infection, the full-length human MYO7A protein was visible; peak expression of the protein occurred around Day 5. 
     EXAMPLE 4 shows in vivo performance of an exemplary overlap vector system, which contains the hMYO7A coding overlap. Six week old shaker-1 (MYO7A null) mice were sub-retinally co-injected with 1 μL of the same preparations of vector A and vector B used in the above in vitro study. Both vectors were delivered at ˜1×10 12  vg/mL. Four weeks post-injection, retinas from treated and untreated eyes were collected and immunohistochemistry (IHC) was performed using an antibody directed against MYO7A (see  FIG.  7 A  and  FIG.  7 B ). Brighter areas indicated MYO7A-specific staining, while the darker areas correspond to the nuclear-specific, DAPI stain. In the treated eye, MYO7A expression was clearly visible, and it appeared to be restricted to photoreceptors—more precisely to the juncture of the photoreceptor inner and outer segments. 
     EXAMPLE 5 shows that AAV dual vectors efficiently deliver oversized genes. 
     Methods and Materials 
     Animals. Shaker-1 mice carrying the 4626SB allele, an effective null mutation (Liu et al., 1999; Hasson et al., 1997), were used on the C57BL6 genetic background, and maintained and genotyped as described (Liu et al., 1999; Gibbs et al., 2003a). They were maintained on a 12-hr light/12-hr dark cycle, with exposure to 10-50 lux of fluorescent lighting during the light phase, and were treated according to federal and institutional animal care guidelines. Homozygous mutants were distinguished from the heterozygous controls by their hyperactivity, head-tossing and circling behavior (Gibson et al., 1995), and/or by a PCR/restriction digest assay. 
     Construction of AAV Vectors. Single-vector platform: AAV vector plasmid, containing the truncated chimeric CMV/chicken β-actin promoter (smCBA) (Haire et al., 2006) and MYO7A cDNA was constructed by removing the full MYO7A cDNA from pEGFP-C2 by EagI and SalI digest, and then ligating into pTR-smCBA-GFP that had been digested with NotI and SalI to remove GFP. The MYO7A cDNA (˜6.7 kb) corresponded to isoform 2 of human MYO7A, and was the same as that described previously by Hashimoto et al. (2007), which was based on the sequence published by Chen et al. (1996) (see SEQ ID NO: 8). MYO7A isoform 2 is 114-kb shorter than isoform 1 (Chen et al., 1996; Weil et al., 1996). Both the MYO7A cDNA, and the resulting junctions were fully sequenced prior to packaging. All vectors intended for in vitro analyses were separately packaged in wild type AAV2, or alternatively in the AAV2 (tripleY-F) capsid mutant vector (Petrs-Silva et al., 2011). 
     As noted above, AAV2-based vectors were chosen for the in vitro experiments due to their increased transduction efficiency relative to other serotypes (Ryals et al., 2011). All vectors were packaged, purified, and titered using standard methods as previously described (Zolotukhin et al., 2002; Jacobson et al., 2006). Human embryonic kidney (HEK293) cells were transfected by the calcium phosphate method with vector plasmid carrying the full-length MYO7A coding sequence of variant 2 (the plasmid used to package fragmented AAV). These transfected cells were then used as a positive control throughout immunoblot analyses to indicate the appropriate size of full-length MYO7A protein. Vector infections were carried out in HEK293 cells with titer-matched AAV vectors. In brief, cells were grown to 60-70% confluency. All vectors were diluted in a balanced salt solution to achieve the desired multiplicity of infection (MOI). If not specifically mentioned, cells were infected at 10,000 genome-containing particles/cell of each vector, resulting in an MOI of 20,000 total for each vector pair. Cells were incubated in medium containing 10% serum for 3 days post-infection at 37 degrees C. under 7% CO 2 , and then analyzed via immunoblot. Titers of 10 12  to 10 13  particles/mL were obtained for different lots of AAV2-MYO7A and AAV5-MYO7A. 
     Oligonucleotide Sequences. For in vivo studies, a human influenza hemagglutinin (HA) tag was added to the 3′ termini of the full-length, simple overlap, trans-splicing, and hybrid 3′ vectors by utilizing a unique BamHI site (P19), and replacing the non-tagged 3′-end with an HA-tagged (P20) version. All constructs were sequence verified by Sanger sequencing. 
     A AV Vector Plasmid Design and Cloning. The full-length coding sequence of MYO7A (human isoform 2; GenBank Accession No. NM_001127180) was cloned into a vector plasmid containing the strong, ubiquitous CMV/chicken β-actin (smCBA) promoter (Haire et al., 2006), a polyadenylation signal, and the AAV2 ITRs. 
     Packaging of this plasmid generated the fAAV vector ( FIG.  22 A ). In all systems, the 5′ vectors shared the smCBA promoter and a 5′ portion of MYO7A, whereas the 3′ vectors contained a 3′-portion of MYO7A, and a bovine growth hormone (bGH) polyadenylation signal. Oligonucleotides used for vector construction are listed in Table 1. The simple overlap contained nucleotides 1 through 3644 of MYO7A cDNA from the ATG in the 5′ vector, and nucleotides 2279 through 6534 in the 3′ vector. The fragments were amplified with oligonucleotides P1 and P3 by polymerase chain reaction (PCR) and cloned into the 5′ vector via NotI and NheI, and the 3′ vector with P3 (AflII) and P4 (KpnI), respectively. 
     The resulting two vector plasmids share 1365 bp of overlapping MYO7A sequence ( FIG.  22 B ). The trans-splicing and hybrid vectors utilize splice junctions composed of either ideal splice donor and acceptor sites derived from AP coding sequence or native MYO7A splice junctions from exons 23 and 24 (Yan et al., 2002). To create the 5′ trans-splicing vector, the splice-acceptor site was amplified using oligonucleotides P5 and P6 (NheI), and the amplicon was then used in a second reaction with oligonucleotide P7 (NsiI) to add a part of the MYO7A coding sequence for cloning. 
     The corresponding 3′ vector was similarly created by amplifying the splice-acceptor site with oligonucleotides P8 (AflII) and P9 in a first PCR, and adding part of the 3′ MYO7A coding sequence with oligonucleotide P10 (AgeI) in a second PCR (see  FIG.  22 C ). The AP hybrid vectors were created by adding 270 bp of AP overlap sequence to the respective trans-splicing vectors (Ghosh et al., 2011). The sequence was amplified by PCR and, in so doing, appropriate restriction endonuclease sites were added. For the 5′ vector oligonucleotides P11 (NheI) and P12 (SalI) were used, while oligonucleotides P13 (NotI) and P14 (AflII) were used for the 3′ vector ( FIG.  22 D ). 
     A fourth vector pair, “native intron hybrid” vector, was also created to exploit the natural sequence in and around intron 23 of MYO7A as a recombination locus, and subsequent splicing signal. The 5′-portion was created by amplifying intron 23 with oligonucleotides P15 and P16 (NheI) first, and then using the resulting amplicon in a second reaction with oligonucleotide P7 (NsiI) to facilitate cloning. The corresponding 3′-vector was constructed by amplifying the intron 23 with oligonucleotides P17 and P18 (AflII), and the resulting amplicon, with oligonucleotide P10 (AgeI) in a second reaction (see  FIG.  22 E ). 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Oligonucleotides used in this study 
               
            
           
           
               
               
               
               
            
               
                 OliGo 
                 5′-3′ sequence (restriction sites underlined) 
                 Restriction site 
                 (SEQ ID NO:) 
               
               
                   
               
               
                 P1 
                 GCG GCGGCCGC CACCATGGTGATTCTTCAGCAGGGGGAC 
                 NotI 
                 SEQ ID NO: 9 
               
               
                   
               
               
                 P2 
                 GCG GCTAGC GAAGTTCCGCAGGTACTTGAC 
                 NheI 
                 SEQ ID NO: 10 
               
               
                   
               
               
                 P3 
                 GCG CTTAAG CAGGTCTAACTTTCTGAAGCTG 
                 AflII 
                 SEQ ID NO: 11 
               
               
                   
               
               
                 P4 
                 GCG GGTACC TCACTTGCCGCTCCTGGAGCC 
                 KpnI 
                 SEQ ID NO: 12 
               
               
                   
               
               
                 P5 
                 GGCACCTAGTGGCTTTGAGGTAAGTATCAAGGTTACAAGAC 
                   
                 SEQ ID NO: 13 
               
               
                   
               
               
                 P6 
                 GCG GCTAGC TCAGAAACGCAAGAGTCTTC 
                 NheI 
                 SEQ ID NO: 14 
               
               
                   
               
               
                 P7 
                 CTTCTTTGTGCG ATGCAT CAAG 
                 Nsii 
                 SEQ ID NO: 15 
               
               
                   
               
               
                 P8 
                 GCG CTTAAG CGACGCATGCTCGCGATAG 
                 AflII 
                 SEQ ID NO: 16 
               
               
                   
               
               
                 P9 
                 CGCCCTCGCTCCAGGTCCTGTGGAGAGAAAGGCAAAG 
                   
                 SEQ ID NO: 17 
               
               
                   
               
               
                 P10 
                 GAACCCGA ACCGGT CCTTG 
                 AgeI 
                 SEQ ID NO: 18 
               
               
                   
               
               
                 P11 
                 GCG GCTAGC CCCCGGGTGCGCGGC 
                 NheI 
                 SEQ ID NO: 19 
               
               
                   
               
               
                 P12 
                 GCG GTCGAC GAAACGGTCCAGGCTATGTG 
                 SalI 
                 SEQ ID NO: 20 
               
               
                   
               
               
                 P13 
                 GCG GCGGCCGC CCCCGGGTGCGCGGCG 
                 NotI 
                 SEQ ID NO: 21 
               
               
                   
               
               
                 P14 
                 GCG CTTAAG GAAACGGTCCAGGCTATGTG 
                 AflII 
                 SEQ ID NO: 22 
               
               
                   
               
               
                 P15 
                 CAGGCACCTAGTGGCTTTGAGGTACCAGGCTAGGGACAGG 
                   
                 SEQ ID NO: 23 
               
               
                   
               
               
                 P16 
                 GCG GCTAGC CGCCTGAGCCCAGAAGTTC 
                 NheI 
                 SEQ ID NO: 24 
               
               
                   
               
               
                 P17 
                 CGCCCTCGCTCCAGGTCCTGAAGGAGACAAGAGGTATG 
                   
                 SEQ ID NO: 25 
               
               
                   
               
               
                 P18 
                 GCG CTTAAG CACCGCTTGTGTTGATCCTC 
                 AflII 
                 SEQ ID NO: 26 
               
               
                   
               
               
                 P19 
                 GCCAGGGAA GGATCC CATG 
                 BamHI 
                 SEQ ID NO: 27 
               
               
                   
               
               
                 P20 
                 GCGG GTACC   TCATGCGTAATCCGGTACATCGTAAGGGTAC TTGCCGCACCA 
                 KpnI 
                 SEQ ID NO: 28 
               
               
                   
                 GGAGCC 
                   
                   
               
               
                   
               
               
                 P21 
                 AGCTTCGTAGAGTTTGTGGAGCGG 
                   
                 SEQ ID NO: 29 
               
               
                   
               
               
                 P22 
                 GAGGGGCAAACAACAGATG 
                   
                 SEQ ID NO: 30 
               
               
                   
               
               
                 Oligonucleotides were used to make 5′ and 3′ vectors of the dual vector platforms (P1-P20). Oligonucleotides were used to characterize the fidelity of the overlap in simple overlap, trans-splicing and AP hybrid vector platforms (P21-P22). Restriction sites used for cloning are underlined and the introduced hemagglutinin (HA) tag is noted in italics (P19). 
               
            
           
         
       
     
     Dual Vector Platform. Two separate vector plasmids were constructed: Vector A contains the strong, ubiquitous “smCBA” promoter and MYO7A cDNA encoding the N-terminal portion. Vector B contains MYO7A cDNA encoding the C-terminal portion and a poly-A signal sequence. Each vector plasmid contained both inverted terminal repeats (ITRs). Using PCR with full-length MYO7A cDNA as a template, the MYO7A cDNA was divided roughly in half with amplicons encompassing nucleotide positions 1 through 3644 (Vector A) and 2279 through 6647 (Vector B) relative to ATG start position 1. The resulting two-vector plasmids shared 1365 bp of overlapping MYO7A sequence, and were 5.0- and 4.9-Kb in length, respectively. This was well within the size limitation of standard AAV vectors. Both vector plasmids were sequence verified and separately packaged by standard AAV production methods (Zolotukhin et al., 2002; Jacobson et al., 2006). The titer of the first lot contained 2.5×10 12  particles/mL of each vector, and the second lot contained 4×10 12  particles/mL of each vector. 
     Reverse Transcription and Characterization of Overlap Region. HEK293 cells were infected with dual vectors, and total RNA was extracted with the RNeasy® kit (Qiagen, Hilden, Germany) according to the manufacturer&#39;s recommended protocol. Two micrograms of RNA were then subjected to DNaseI (NEB) digestion for 30 min at 37 degrees Celsius, followed by heat inactivation at 75 degrees Celsius for 10 min. Reverse transcription to cDNA was achieved with the SuperscriptIII® kit (Life Technologies, Grand Island, N.Y., USA) according to the standard protocol utilizing the oligo dT primer. Two microliters of cDNA was used as template in a PCR (95 degrees Celsius for 3 min. initial denature, 35 cycles of 95 degrees Celsius for 45 sec., 55 degrees Celsius for 45 sec., 72 degrees Celsius for 12 min., and a final 72 degrees Celsius for 15 min.) using oligonucleotide primers P21 and P22 (see Table 1). Annealing sites for these primers are located 5′ and 3′, respectively, of the area of cDNA overlap (in other words, outside the region of overlap) in the simple overlap and hybrid vector pairs. The 3′-primer annealed to sequence that was complimentary to the bGH polyA. Resulting products were digested with either PpuMI or BglII, separated on a 1.5% agarose gel, and subsequently analyzed on a UV screen. Separately, products were digested with KpnI and AgeI, and subsequently cloned into a pUC vector for sequencing of the entire overlap region. M13˜forward and reverse primers that were specific for the vector were used to obtain sense and antisense reads resulting in an 140 bp overlap of the sense and antisense reads. To demonstrate that these methods were capable of detecting aberrant sequence (for example, for quality control), a MYO7A sequence was generated using either an artificial insertion (HindIII fill-in at position 2635) or a point mutation (T→C) at position 2381, and the analyses were repeated. 
     Viral Delivery in Vitro. HEK293A cells (Invitrogen), grown in DMEM with 10% FBS and 1×NEAA and Pen/Strep (Invitrogen) were plated in 6 well-plates. The next day cells were incubated, at 37 degrees Celsius and 5% CO 2 , with AAV2- and AAV5-MYO7A at an MOI of 10,000 viral particles/cell in 500 μL of complete medium, containing also 40 μM of calpain inhibitor (Roche, Pleasanton, Calif., USA). Two hours later complete medium was added. The next day, the medium was changed and cells were incubated for an additional 48 hrs. Alternatively, some cells were transfected with 1 μg of vector pTR-smCBA-MYO7A, complexed with Lipofectamine 2000 (ratio 1:3), according to the manufacturer&#39;s instructions (Invitrogen). 
     Primary mouse RPE cells were derived from P14-P16 MYO7A-null animals and cultured in 24-well dishes, as described (Gibbs et al., 2003a; Gibbs and Williams, 2003b). After 48 hrs. in culture, cells were transduced with viruses. Cells were incubated in 100 μL of complete medium containing 40 μM of calpain inhibitor, and 10,000 viral particles/cell from full-strength AAV stocks. After 2 hrs, 400 μL of complete medium was added to each well, and incubated overnight. The medium was changed the following day, and cells were incubated for an additional 48 hrs. 
     ARPE19 cells (American Type Culture Collection, Manassas, Va., USA) were cultivated in DMEM/F-12 with 10% FBS and split into 24-well plates with glass coverslips. Cells were grown to confluency and then transduced in the same manner, as were the primary RPE cells. 
     MYO7A expression analysis by Western blot and Immunofluorescence. HEK293A and primary mouse RPE cells that were transduced with AAV-MYO7A were collected 3 days post-transduction. For western blot analyses, cells were collected and lysed in 20 mM TRIS, pH 7.4, 5 mM MgCl 2 , 10 mM NaCl, 1 mM DTT and 1× protease inhibitor cocktail (Sigma-Aldrich Chemical Co., St. Louis, Mo., USA). Equivalent amounts of total protein were separated on a 7.5% SDS-PAGE gel. After transfer, blots were blocked with 5% non-fat milk, and probed with mouse anti-MYO7A antibody, generated against residues 927-1203 of human MYO7A (Developmental Studies Hybridoma Bank, Iowa City, Iowa USA) (Soni et al., 2005), and mouse anti-actin antibody (Sigma-Aldrich) as a loading control. 
     Immunofluorescence was performed with ARPE19 and mouse RPE primary cells, 3 days after infection. Cells were fixed in 4% formaldehyde, blocked with blocking solution (0.5% BSA/0.05% saponin in PBS), incubated with the mouse anti-MYO7A followed by goat anti-mouse Alexa-568 (Molecular Probes, Carlsbad, Calif., USA). Coverslips were mounted with mounting medium containing DAPI (Fluorogel II, Electron Microscopy Sciences, Hatfield, Pa., USA) and visualized on a Leica confocal system. 
     Protein extraction and immunoblotting. Transfected and infected HEK293 cells were harvested and washed twice in PBS and processed as previously reported with minor modifications (Boye et al., 2012). The cells were lysed by 3×30 second pulses of sonication in 200 μL of sucrose buffer (0.23 M sucrose, 2 mM EDTA, 5 mM Tris-HCl, pH 7.5) containing protease inhibitors (Roche, Mannheim, Germany). Unlysed cells and cell debris were removed by centrifugation at 14,000 rpm for 10 min. The protein concentration of the supernatant was measured with BCA (Thermo Fisher Scientific, Rockland, Ill., USA). Equal amounts of protein were then loaded on 7.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis gels (BioRad, Hercules, Calif., USA) and transferred in CAPS buffer (pH 11) onto PVDF membranes (Millipore, Billerica, Mass.). Blots were then labeled with antibodies against MYO7A (monoclonal antibody raised against amino acids 11-70 of human MYO7A; Santa Cruz, Dallas, Tex., USA; 1:1000) or HA (MMS-101P; Covance, Gaithersburg, Md., USA; 1:500) and β-actin (ab 34731; Abcam, Cambridge, Mass., USA; 1:5000). For visualization with the Odyssey system (Li-Cor, Lincoln, Nebr., USA), an anti-mouse and an anti-rabbit secondary antibody conjugated with CW800 and IR680 dyes (Li-Cor), respectively, were used. Semi-quantitative densitometric measurements were performed with Odyssey acquisition and analysis software (Li-Cor). The dual-color images were separated in their respective channels and converted to gray scale for presentation purposes. Size markers present in one channel of each blot were added to both channels for visualization of protein sizes. 
     Viral Delivery in vivo. Mice were anesthetized with 2.0-3.0% isoflurane inhalation. The pupils of the animals were dilated with 1% (wt./vol.) atropine sulfate and 2.5% phenylephrine. A local anesthetic (0.5% proparacaine hydrochloride) was also administered. A sclerotomy in the temporal limbus was performed with a 27-Ga needle. A 32-Ga blunt needle, attached to a microsyringe pump (WPI, Sarasota, Fla., USA) was inserted and 1 μL of viral solution was injected into the ventral subretinal space of P14-P16 animals. Retinal detachment was visualized under a dissecting microscope, and registered as indication of a positive subretinal injection. One microliter of the following AAV8(Y733F)-based vectors was injected subretinally in one eye of C57BL/6 mice: single fAAV (1×10 13  vg/mL), front and back half “hybrid” vectors combined equally (each vector=1×10 13  vg/mL), or front and back half “simple overlap” vectors combined equally (each vector=1×10 13  vg/mL). Subretinal injections were performed as previously described (Timmers et al., 2001). Further analysis was carried out only on animals that received comparable, successful injections (&gt;60% retinal detachment with minimal surgical complications). 
     Light Microscopy and Immunoelectron Microscopy of Retinas. Eyecups were processed for embedment in either LR White or Epon, and semithin and ultrathin sections were prepared. Semithin sections were stained with toluidine blue and visualized on a Leica confocal system. Ultrathin sections were labeled with purified MYO7A pAb 2.2 (Liu et al., 1997) and monoclonal anti-opsin (1D4, R. Molday), followed by gold-conjugated secondary antibodies (Electron Microscopy Sciences), as described previously (Lopes et al., 2011). Negative control sections processed at the same time included those from MYO7A-null retinas, and, as positive control, WT animals were used. 
     MYO7A immunogold density was determined on sections of age-matched WT, MYO7A-null retinas and retinas of MYO7A-null animals that had been injected with AAV-MYO7A at P14-16 and dissected three weeks later. For quantification of the immunolabel, all of the gold particles in a complete section of each RPE cell were counted. The area of each cell&#39;s profile was determined using ImageJ software. For background labeling, the concentration of label in sections of untreated MYO7A-null animals was measured. Data were expressed with this background labeling subtracted. 
     The concentration of MYO7A and opsin immunogold labeling in the connecting cilia of photoreceptor cells was determined by counting gold particles along longitudinal profiles of connecting cilia and measuring the length of each profile. Analysis and quantifications were performed in a minimum of three different retinas, from three different animals. Statistical analysis was performed using one-tail Student&#39;s t-test. 
     Six weeks post-injection, C57BL/6 mice were enucleated and their eyes processed and immunostained as previously described (Boye et al., 2011) with minor modifications. Retinas were immunostained with an antibody specific for hemagglutinin (HA) (monoclonal Ab clone 12CA5; Roche), counterstained with DAPI, and imaged with a spinning disk confocal microscope (Nikon Eclipse TE2000 microscope equipped with Perkin Elmer Ultraview Modular Laser System and Hamamatsu O-RCA-R2 camera). Images were obtained sequentially using a 20× (air) objective lens. All settings (exposure, gain, laser power) were identical across images. All image analysis was performed using Volocity 5.5 software (Perkin Elmer, Waltham, Mass., USA). 
     Results 
     AA V-MYO7A single vector preparations. AAV vector plasmid was engineered to contain a truncated chimeric CMV/chicken β-actin promoter, smCBA (Haire et al., 2006) and the 6.7-kb cDNA encoding the full-length isoform 2 of human MYO7A (NCBI #NM_001127180) ( FIG.  10 A ). The smCBA promoter exhibits the same tropism and activity in mouse retinas as that of the full-length CBA promoter (Haire et al., 2006; Pang et al., 2008). Titers of 10 12  to 10 13  particles/mL were obtained for different lots of AAV2-MYO7A and AAV5-MYO7A. A concentration of 10 12  particles/mL was regarded as the standard concentration (lx), from which dilutions were made. The experiments were performed with virus obtained from three separate preparations. No differences in expression or phenotype correction, as described below, were observed among the different lots for AAV2-MYO7A or AAV5-MYO7A at a given concentration. 
     MYO7A Expression in Cell Culture. Transduction of primary cultures of MYO7A-null RPE cells with 1× single AAV2-MYO7A or AAV5-MYO7A resulted in the expression of a polypeptide that, by western blot analysis, had an apparent mass that was comparable to that of WT MYO7A protein, and was present at similar levels to that found in primary cultures of MYO7A +/−  RPE cells ( FIG.  10 B ). Likewise, a single band of appropriate size was detected on western blots of HEK293A cells. Immunofluorescence of the primary RPE cells showed that the MYO7A protein, resulting from 1× single AAV-MYO7A treatment of MYO7A-null cells, had a subcellular localization pattern that was comparable to that of endogenous MYO7A in control cells, indicating the generation of appropriately targeted protein ( FIGS.  10 C- 10 F ). ARPE19 cells were also infected with 1× or diluted (1:100) AAV2-MYO7A or AAV5-MYO7A, and compared with non-treated cells. An increase in MYO7A immunofluorescence was detected in the treated cells, and the intracellular localization of the label was comparable to that in untreated cells ( FIGS.  17 A- 17 F ). 
     Localization of MYO7A in Vivo. Most retinal MYO7A is found in the RPE (Hasson et al., 1995), however, the protein is also present in the connecting cilium and pericilium of the photoreceptor cells (Liu et al., 1997; Williams, 2008). A diagram illustrating this distribution and the retinal functions of MYO7A has been published in a recent review (Williams and Lopes, 2011). 
     Three weeks following injection of 1×AAV2-MYO7A or AAV5-MYO7A into the subretinal space of MYO7A-null mice, retinal tissue was examined by immunoelectron microscopy to test for MYO7A expression. Immunogold label was evident in the photoreceptor cells, where it was localized in the connecting cilium and pericilium, comparable to that in WT retinas ( FIGS.  11 A-E ). Label was also present throughout the RPE cells, particularly in the apical cell body region ( FIG.  11 F ,  FIG.  11 F- 1   ,  FIG.  11 G , and  FIG.  11 G- 1   ; see  FIGS.  18 A- 18 D  for controls), as found in WT retinas (Gibbs et al., 2004; Liu et al., 1997). 
     MYO7A has a similar distribution in both rod and cone photoreceptor cells (Liu et al., 1999). To test whether treatment with AAV-MYO7A also affected cone photoreceptor cells, it was determined whether MYO7A was also present in the ciliary region of cone photoreceptors. Double immunoEM of treated retinas was performed, using a MYO7A antibody together with an antibody specific for rod opsin. Although there are only a small number of cones with aligned connecting cilia found in each ultrathin section, MYO7A immunogold label was evident in the connecting cilium and periciliary region of these cones, which were identified by lack of rod opsin labeling in their outer segments (in contrast to the surrounding rod outer segments) ( FIG.  11 H  and  FIG.  11 I ). Hence, AAV2-MYO7A and AAV5-MYO7A can transduce cone as well as rod photoreceptor cells. 
     Dose-dependent MYO7A expression in photoreceptor and RPE cells. To determine the levels of MYO7A expression following treatment with different concentrations of AAV2-MYO7A and AAV5-MYO7A (lx, 1:10 or 1:100 dilutions), MYO7A immunogold labeling was quantified in EM images, taken within 1.4 mm of the injection site. Reliable detection of MYO7A in the photoreceptor cells, where its distribution is limited to the connecting cilium and pericilium, requires the higher resolution provided by electron microscopy (Liu et al., 1997). Immunogold particle density was measured in images of the photoreceptor connecting cilium and pericilium, shown in complete longitudinal section (from the basal bodies to the base of the outer segment), and in images showing the RPE cells in apical to basal section. Particle density was expressed as particles per length of cilium for the photoreceptor cells (each connecting cilium is ˜1.2 m long), and as particles per area for the RPE cells (the entire area between the apical and basal surfaces was included). Particle density is dependent on exposure of epitopes on the surface of the section, and, as such, provides a relative linear measure of antigen density under the conditions used here (for example, grids were etched and labeled in an identical manner, and the labeling was not so dense as to be affected by steric hindrance). 
     Treatment with 1×AAV2-MYO7A or AAV5-MYO7A resulted in 2.5-2.7 times the density of immunolabel in the photoreceptor cilium, compared with that found in WT retinas, while the 1:10 and 1:100 dilutions resulted in a density of immunolabel that was more comparable to WT levels ( FIG.  11 J ,  FIG.  11 L , and  FIG.  19   ). Quantification of immunogold label in the RPE showed that injection of AAV2-MYO7A resulted in 2.7 times more label than in WT, with the 1:10 and 1:100 dilutions showing no significant difference ( FIG.  11 K ). In contrast, the level of MYO7A immunolabel in the RPE of retinas injected with AAV5-MYO7A varied in relation to virus titer, with the full dose virus effecting 2.2-fold more MYO7A than that found in WT RPE, the 1:10 dilution effecting WT levels, and the 1:100 dilution resulting, on average, ˜60% of WT levels ( FIG.  11 M ). 
     These counts of labeling density indicate that 1×AAV-MYO7A resulted in more than double the normal level of MYO7A expression in both the photoreceptor and RPE cells. The distribution of MYO7A was not affected by this overexpression in the photoreceptor cells. In the RPE cells, the overall distribution of MYO7A was comparable to WT, with a higher concentration in the apical cell body region. However, with 1×AAV2-MYO7A or 1×AAV5-MYO7A, the proportion of MYO7A that was associated with melanosomes was only 55% of that in WT RPE. This difference is possibly because the proteins that link MYO7A to the melanosomes, MYRIP and RAB27A (Klomp et al., 2007; Lopes et al., 2007), may have remained near WT levels, and thus limited the absolute amount of MYO7A that could associate with the melanosomes. 
     Despite the overexpression of MYO7A, no pathology was evident in retinas, up to 3 months after injection of 1× (or 1:10) AAV2-MYO7A. However, two out of six retinas injected with 10 13  particles/mL of AAV5-MYO7A (for example, 10×) showed evidence of photoreceptor cell loss across the retina after 3 weeks (AAV2-MYO7A was not tested at this titer) ( FIG.  19   ). 
     Correction of melanosome localization in the RPE. In MYO7A-mutant mice, melanosomes are absent from the apical processes of the RPE cells (Liu et al., 1998). This mutant phenotype is evident at all neonatal ages, and is due to loss of actin-based transport of the melanosomes by the myosin 7a motor (Gibbs et al., 2004). Three weeks following injection of 1×AAV2-MYO7A or AAV5-MYO7A into the subretinal space of MYO7A-null mice, melanosomes were observed to have a normal distribution in all RPE cells near the site of injection (within 1.4 mm) (n=10 each for AAV2-MYO7A and AAV5-MYO7A) ( FIGS.  12 A- 12 C ). Well away from the injection site, a mixture of corrected and uncorrected RPE cells was evident, while, at the periphery of the retina, the cells all exhibited the MYO7A-mutant phenotype, indicating lack of correction in this region ( FIGS.  12 D- 12 F ). The correction of melanosomes was still evident in retinas that were fixed 3 months after injection ( FIG.  20   ). Correction was also observed in all eyes injected with 1:10 dilution AAV2-MYO7A (n=6) or AAV5-MYO7A (n=6), as well as in all eyes injected with 1:100 dilution AAV2-MYO7A (n=6) or AAV5-MYO7A (n=6), although with the 1:100 dilution some of the RPE cells near the site of injection were not corrected. 
     Correction of opsin distribution. MYO7A-mutant mice have an abnormal accumulation of opsin in the connecting cilia of the photoreceptor cells, a phenotype that is evident by immunoEM with opsin antibodies (Liu et al., 1999). This mutant phenotype suggested that myosin 7a functions in the vectorial delivery of opsin to the outer segment (Liu et al., 1999). Quantification of immunogold opsin labeling in the connecting cilia, demonstrated that this phenotype was corrected with 1×AAV2-MYO7A or AAV5-MYO7A ( FIG.  13    and  FIGS.  21 A- 21 D ). This analysis also showed phenotype correction with 1:100 dilutions, although the data indicated that a full WT phenotype was not achieved ( FIG.  13   ), despite WT levels of MYO7A ( FIG.  11 J  and  FIG.  11 L ), suggesting that some of the MYO7A may not be fully functional. 
     AAV2-MYO7A dual vector preparations. The preceding results demonstrate that a single AAV vector is capable of delivering functional MYO7A to the RPE and photoreceptor cells in vivo. Because the size of smCBA-MYO7A is ˜2 kb larger than the nominal carrying capacity of an AAV (Grieger and Samulski, 2005), this transduction may involve undefined fragmentation of the smCBA-MYO7A cDNA followed by reassembly of plus and minus cDNA strands after delivery to the cell as shown for other large genes (Dong et al., 2010; Lai et al., 2010; Wu et al., 2010). To evaluate whether two AAV vectors containing defined, overlapping fragments of MYO7A cDNA (1365 bases) were also capable of mediating full-length MYO7A expression, an AAV2-based dual vector system ( FIG.  14 A- 1    and  FIG.  14 A- 2   ) was developed. Two separate lots of the AAV2-MYO7A (dual vector) were prepared, each containing equal concentrations of AAV2-smCBA-MYO7A(5′-half) and AAV2-MYO7A(3′-half). The titer of the first lot contained 2.5×10 12  particles/mL of each vector, and the second lot contained 4×10 12  particles/mL. 
     MYO7A expression with AAV2 dual vectors. Western blot analysis of primary cultures of MYO7A-null RPE cells, infected with AAV2-MYO7A (dual vector) of either lot, showed that the cells expressed a MYO7A-immunolabeled polypeptide of comparable mass to that of WT MYO7A ( FIG.  14 B ). However, the expression level of MYO7A in the MYO7A-null RPE cells was significantly less than that found in primary cultures of MYO7A +/−  RPE cells (cf. lanes 2 and 3 in  FIG.  14 B ), unlike that found for the single AAV2 or AAV5 vectors ( FIG.  10 B ). Quantitative analysis of western blots showed that MYO7A-null RPE cells, transduced with the single vectors (lx), AAV2-MYO7A or AAV5-MYO7A, or with AAV2-MYO7A (dual vector), expressed MYO7A at levels that were 82%, 111%, and 10%, respectively, of the level of MYO7A in MYO7A +/−  RPE cells. 
       FIG.  16    is a Western blot using the same dual vector system as above except in an AAV8 serotype.  FIG.  16    shows expression level of MYO7A using the dual vector system that was nearly equivalent to the wild type MYO7A expression level. While the reasons for the discrepancy are unclear, much better results were obtained by the inventors using the dual vector systems than were obtained by several outside collaborators. Wild-type-like levels of MYO7A expression were observed in shaker-1 retinas following injection with dual-AAV8(Y733F) vectors. Thus, very good expression of MYO7A with the dual AAV platform has been achieved. 
     Immunofluorescence of primary MYO7A-null RPE cells, infected with AAV2-MYO7A (dual vector), showed that a few cells scattered throughout the culture exhibited very high levels of MYO7A, but all other cells contained insignificant levels ( FIGS.  14 C- 14 E ). The cells overexpressing MYO7A typically had altered morphology, suggesting that the high levels of MYO7A may be toxic. Similarly, immunofluorescence of ARPE19 cells, infected with AAV2-MYO7A (dual vector), resulted in a minority of cells that were labeled intensely with MYO7A antibody, with most of the cells appearing to express only endogenous levels of MYO7A ( FIG.  14 F  and  FIG.  17 F ). 
     Immunolabeling of retinas, prepared 3 weeks after subretinal injection with AAV2-MYO7A (dual vector) of either lot, also showed only a few RPE cells and photoreceptor cells with clear MYO7A expression, although significant overexpression was not evident in this in vivo experiment. Immunogold particle counts from images of ultrathin sections were used to quantify the level of MYO7A expression in MYO7A-null retinas that were treated with the second lot of AAV2-MYO7A (dual vector). Within 1.4 mm of the injection site, MYO7A immunolabeling of the connecting cilium and pericilium of the photoreceptor cells was a mean of 48% of that in WT retinas: 2.8 particles/μm (n=3 retinas) compared with 6.5 particles/μm for WT (n=3 retinas). The mean label density in apical-basal sections of the RPE was 35% of that in WT retinas: 11 particles/100 μm 2  compared with 31 particles/100 μm 2  for WT. However, it was clear that these lower means were achieved by some cells expressing near normal amounts of MYO7A and the majority expressing very little; over half the cells had fewer than 10 particles/100 μm 2  ( FIG.  14 G ). 
     Correction of MYO7A-mutant phenotypes with AAV2 dual vectors. Eyes were analyzed for correction of melanosome localization and ciliary opsin distribution within 1.4 mm of the injection site. With either lot of AAV2-MYO7A (dual vector), some RPE cells (29% for lot 1 treatment [n=6 retinas], 35% for lot 2 treatment [n=9 retinas]) were observed to have a normal apical melanosome distribution, but most of the cells in this region retained the MYO7A-mutant phenotype, resulting in a mosaic effect ( FIG.  15 A ) that contained a much lower proportion of corrected cells than that observed with a 1:100 dilution of either of the single vectors. The only correction observed in 3 eyes injected with a 1:10 dilution of AAV2-MYO7A (dual vector) (first lot), was in 18% of the RPE cells in one of the retinas. With full-strength of AAV2-MYO7A (dual vector) (second lot), opsin immunogold density averaged 3.2±0.4 particles/μm of cilium length, which was reduced from untreated retinas (4.2±0.8 particles/μm; p=0.003), but still greater than WT levels (1.1±0.2 particles/μm), suggesting that most cells were not corrected. 
     Using immunoelectron microscopy, a correlation between phenotype correction and the expression level of MYO7A was identified (determined by the mean concentration of immunogold particles in an apical-basal section of each RPE cell) ( FIGS.  15 B- 15 E ). From the eyes injected with AAV2-MYO7A (dual vector) (second lot), it was shown that the corrected RPE cells contained a mean of 108% of the WT level of MYO7A (the minimum level was 82%). RPE cells that were not corrected contained a mean of 26% of the WT level of MYO7A (the maximum level was 92%). While these data showed that higher expression of MYO7A is correlated with phenotype correction ( FIG.  15 F ), it also indicated that some of the labeled MYO7A protein was not functional, given that melanosomes are localized normally in mice that are heterozygous for the MYO7A-null allele and have only ˜50% of the WT level of MYO7A. 
     Expression of MYO7A with simple overlap vectors. AAV2-based simple overlap vectors were evaluated in vitro at a variety of MOIs to evaluate how the concentration of vector pairs related to MYO7A expression. How levels of MYO7A changed over time was also evaluated in infected cells. HEK293 cells were infected with simple overlap vector pairs packaged in AAV2(tripleY-F) vector ( FIG.  23 A ). A preliminary co-infection with AAV2(tripleY-F) simple overlap vectors (MOI of 10,000 for each vector) indicated that MYO7A is expressed, and that migration of the protein on gel is identical to a full-length transfection control ( FIG.  23 A ). Coinfection at MOIs of 400, 2000, and 10,000 of each vector shows that the efficiency of the simple overlap system is proportional to the amount of 5′ and 3′ vectors used ( FIG.  23 B ). MYO7A expression increased as a function of incubation time up to 5 days post-injection in HEK293 cells ( FIG.  23 C ). The visible expression decline was because of a reduction of viable cells in the culture vessel at the later times. 
     Comparison of fAAV-MYO7A to dual-AAV-MYO7A expression and evaluation of AA V serotype efficiency. Previously, it was shown that fragmented AAV encoding MYO7A was able to ameliorate the retinal phenotype of the shaker1 mouse (Colella et al., 2013; Lopes et al., 2013; Trapani et al., 2013). To provide a basis for comparison dual-AAV-vector expression was evaluated relative to fAAV in vitro. After infection in HEK293 cells, all dual vector systems expressed MYO7A more efficiently than fAAV ( FIG.  24   ). The AP hybrid platform showed the strongest expression, followed by the simple overlap system. 
     Other studies have shown, in the context of a conventionally sized DNA payload, that the transduction efficiency and kinetics of AAV2(tripleY-F) vectors are increased relative to standard AAV2 both in vitro and in vivo (Li et al., 2010; Markusic et al., 2010; Ryals et al., 2011). The efficiency of AAV2 versus AAV2(tripleY-F) dual vectors was directly compared in HEK293 cells. Surprisingly, standard AAV2-mediated MYO7A expression was higher than that seen with titer-matched AAV2(tripleY-F) ( FIG.  24   ). Identical results were obtained when comparing different AAV2 and AAV2(tripleY-F) dual vector preparation packaged with identical vector plasmid. 
     Comparison of relative efficiencies and specificity of full-length MYO7A expression. To quantitatively evaluate the relative expression efficiencies of the dual vector platforms and to assess specificity of full-length protein, HEK293 cells were infected with either the 5′ and 3′ AAV2-based vector pairs combined or the corresponding 5′ vector alone. An additional hybrid vector pair was included that incorporated native MYO7A intronic sequence (intron 23) that served as overlapping sequence and provided appropriate splicing signals. All 5′ vectors produced low amounts of a defined, less than full-length peptide detectable on Western blot with the exception of the simple overlap vector ( FIG.  25 A ). However, the trans-splicing and the AP hybrid platforms revealed a distinct decrease of this undesired product when the 3′ vector was added to the sample ( FIG.  25 A ). The native intron hybrid platform also showed this secondary band on Western blots, again suggestive of a truncated protein originating from the 5′ vector alone. In contrast to all other platforms tested, this band intensity increased with the addition of the 3′ vector. Each platform&#39;s relative ability to promote reconstitution was compared by quantifying the amount of 5′ vector-mediated truncated protein product in the presence or absence of the respective 3′ vector ( FIG.  25 B ). Full-length MYO7A expression on Western blot was then quantified relative to transfection control ( FIG.  25 C ). AP hybrid-mediated MYO7A was the strongest followed by simple overlap, trans-splicing, and native intron hybrid ( FIG.  25 C ). 
     Characterization of the overlap/splice region of the expressed MYO7A. To characterize the fidelity of the mRNA arising from dual vectors, HEK293 cells were infected with dual vectors and RNA extracted, reverse transcribed, and subjected to PCR utilizing primers binding upstream of the overlap region and in the bGH polyA signal region producing a 4.5 kb PCR fragment ( FIG.  26 A ). An identically treated sample not containing reverse transcriptase was used as control for chromosomal DNA contamination. Plasmid containing the full-length MYO7A coding sequence was used as positive control for PCR. A preliminary screen of AAV-mediated MYO7A mRNA was performed by analyzing the pattern of fragment migration on agarose gel following restriction endonuclease digests with PpuMI and BglII ( FIG.  26 A ). Identical banding patterns, consistent with the predicted pattern (PpuMI: 1591, 876, 556, 548, 541, 238, 168, 42, and 36 bp; BglII: 1335, 1074, 827, 583, 360, 272, and 146 bp), were observed following digests of amplicons from each dual vector platform tested, indicating that no gross alterations (deletions/insertions) occurred as a consequence of either homologous recombination of vector pairs and/or RNA splicing ( FIG.  26 B ). To further characterize the fidelity of the overlap region, a fragment containing the complete overlap area (1829 bp) was restricted and cloned into pUC57 ( FIG.  26 A ). Sequencing results of 10 clones picked at random per vector platform revealed that the overlap region was 100% identical to the consensus/predicted MYO7A sequence ( FIG.  26 C ). This indicated that, in the context of the simple overlap platform, homologous recombination was accurate. Additionally, in the context of trans-splicing vectors, accurate splicing occurred. Finally, for the AP hybrid vectors, a combination of accurate homologous recombination and/or splicing took place. To determine whether this protocol was capable of detecting aberrant sequence in reconstituted MYO7A, a sequence that contained either an insertion of a HindIII recognition site (TAGC) at position 2635 or a point mutation (T-C) at position 2381 was also generated. 
     MYO7A expression mediated by dual vectors in mouse retina. To investigate the expression of MYO7A from the two best performing dual vector platforms in vivo, C57BL/6J mice were subretinally injected with 1×10 10  vector genomes per eye of simple overlap and AP hybrid systems packaged in AAV8(733) and analyzed 4 weeks later by Western blot and immunohistochemistry. AAV8(733)-fAAV-MYO7A vector was also injected to provide a basis for comparison. To distinguish between endogenous MYO7A and exogenous expression mediated by vectors, sequence coding for an HA tag was added to the C prime terminus of the MYO7A cDNA in all constructs. Resulting retinas were immunostained for HA to reveal that fAAV vector along with both dual vector platforms mediated expression of MYO7A in photoreceptors and RPE. A recent report concluded that simple overlap vectors were more efficient for gene transfer to the RPE than photoreceptors (Trapani et al., 2013). Simple overlap-mediated MYO7A expression was observed in both RPE and photoreceptors. In contrast to previous results showing “spotty” MYO7A expression mediated by AAV2-based simple overlap vectors (Lopes et al., 2013), it was found, when packaged in AAV8(733), that simple overlap vectors mediated MYO7A expression in the majority of RPE and photoreceptor cells. Photoreceptor degeneration/outer nuclear layer thinning was apparent in eyes injected with the AP hybrid vector system. Despite the observed degeneration, AP hybrid-mediated MYO7A was clearly detected in residual PR cell bodies and RPE and was sufficient to be detected by immunoblot. By Western blot analysis using HA antibody, simple overlap-mediated MYO7A was present in just detectable amounts. In contrast, fAAV-mediated protein levels were insufficient to be detected in this assay. Using an antibody against MYO7A, immunoblot of WT mouse retina revealed that both endogenous MYO7A and dual vector-mediated, HA-tagged MYO7A migrated similarly. 
     Discussion 
     In this example, it was shown that dual AAV vectors with defined genetic payloads can be used to deliver a large transgene in vitro and in vivo. The initial experiments using the simplest of all dual vector platforms revealed that efficiency of AAV2-based simple overlap vectors is proportional to the amount of 5′ and 3′ vectors used and that MYO7A expression mediated by this system increased as a function of incubation time in HEK293 cells. Next, three distinct dual vector platforms were evaluated and compared to single, fragmented fAAV vector in vitro. All dual vectors analyzed drove higher levels of MYO7A expression than fAAV. Of all platforms tested, a hybrid vector system containing overlapping, recombinogenic sequence and splice donor/acceptor sites from the AP gene (AP hybrid) was the most efficient. 
     Regarding the specificity with which the dual vector platforms express the correct-sized gene product, it was noted in vitro that trans-splicing and hybrid dual vector platforms generated an additional band of lower molecular weight as detected by immunoblot (monoclonal antibody used was raised against the amino terminus MYO7A). The expression of this truncated protein product was much more pronounced for infections with 5′ vectors alone. 
     After entry into the host cell, the virus capsid is removed and the single-stranded DNA payload is released. The ITRs carried by the single strand serve as primer for DNA polymerases to produce a double strand. The resulting circular intermediates consist mainly of monomers that, over time, convert into multimeric concatemers through intermolecular recombination (Duan et al., 1998; Yang et al., 1999). The dual vector systems of this disclosure utilize this strategy to achieve full-length protein expression. A limiting factor lies in the fact that the highly recombinogenic ITRs flanking the expression cassettes are identical in nature leading to a random recombination and consequently a random orientation of the vector parts relative to each other. This random recombination inevitably results in reduced efficiency because only concatemers that have the two vector parts in 5′ to 3′ orientation are able to express the full-length protein. This concatemerization over time is consistent with the observation that the amount of single-vector product is reduced in favor of the full-length protein when both 5′ and 3′ vectors are combined. Interestingly, the simple overlap system does not generate truncated product, even when only the 5′ vector is used for infections. In contrast to the trans-splicing and hybrid vectors, there is virtually no intervening sequence between the end of the MYO7A coding sequence and the right-hand ITR. 
     Notably, in this disclosure, it was found that the sequence in the overlap region of all dual vectors tested in vitro was 100% identical to the consensus/predicted MYO7A sequence. This indicates that homologous recombination and/or splicing was accurate in each dual vector platform. 
     Similar to the in vitro results, the highest levels of MYO7A expression was found in retinas of mice subretinally injected with AAV8-based AP hybrid vectors (as assessed by probing for HA on Western blot). Notably, no truncated proteins were evident in retinas expressing either simple overlap or AP-hybrid mediated MYO7A. The reason for this observed difference remains to be elucidated but may involve differences in the DNA repair machinery that mediate recombination in actively dividing cells versus post-mitotic photoreceptors/RPE (Hirsch et al., 2013). Dual vector-mediated MYO7A-HA expression was observed in the photoreceptors and RPE of WT mice, locations where MYO7A is thought to have a functional role (Williams and Lopes, 2011). In eyes injected with AP hybrid vectors, marked thinning of the outer nuclear layer was observed. It has previously been shown that vector-mediated overexpression of MYO7A leads to retinal toxicity (Hashimoto et al. 2007). Taken together with the high efficiency of transduction observed in vitro for the AP hybrid platform, the most likely explanation for the observed pathology is excessive production of MYO7A. Despite the marked degeneration, significant amounts of AP hybrid-mediated, full-length MYO7A-HA were detected on Western blot. As high concentrations of vectors were used in these experiments, a simple solution to circumvent cytotoxicity could be to reduce vector genomes injected or replace the strong, ubiquitous smCBA promoter with an endogenous or homologous promoter, and/or a promoter with attenuated strength; or reduce expression of undesired products, like the observed protein expressed from the 5′ vectors alone in vitro. However, it was noted that only full-size MYO7A-HA was apparent on Western blot of the AP hybrid-treated retina. 
     With the goal of developing an AAV-based treatment for USH1B, animal models of this disease have provided an abundance of useful information. Similar to previous observations that fAAV-MYO7A and simple-overlap, dual vectors were capable of restoring melanosome migration and opsin localization in the shaker1 mouse (Lopes et al., 2013), a recent study by an independent lab confirmed the usefulness of the vectors disclosed herein, when it was reported that they were capable of restoring the ultrastructural retinal phenotypes in the animal model. Notably, shaker1 mice lack retinal degeneration, and the severe functional abnormalities seen in USH1B patients (Liu et al., 1997). This fact renders in vivo analysis of therapeutic outcomes in the shaker retina problematic. Alternative animal models for evaluating a treatment for this devastating disease may be useful in adaptation of the present methods to human clinical use. 
     The results presented here also demonstrated that MYO7A can be efficiently expressed using dual-AAV-vector systems. The platforms containing overlapping elements, namely, the simple overlap system, and the AP hybrid system were both highly efficient. AP hybrid vectors showed the strongest expression of all systems tested, with little observable truncated protein in vitro and none observed in vivo. Simple overlap vectors showed good expression and were the most specific (no truncated protein products were observed) even when the 5′-only vector was used to infect cells. AAV has emerged as the preferred clinical vector and it efficiently transduces both photoreceptors and RPE. Because it has now been demonstrated that MYO7A sequence fidelity is preserved following recombination and/or splicing of dual-AAV-vector platforms and because only full-length MYO7A was detectable in mouse retinas injected with dual vectors, the dual-AAV-vector strategy presented herein represents a valid option for the treatment of retinal disorders associated with mutations in large genes such as USHIB. 
     Nucleotide Sequences of the Vectors Used in Examples 1-5 
       
     
       
         
           
               
               
            
               
                 SEQ ID NO: 1 is the nucleotide sequence of the first generation front-half vector of the 
                   
               
               
                 overlap system (i.e., AAV-smCBA-hMYO7A-NTlong; “hMyo7a coding overlap vector 
               
               
                 A”); 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCAATTCGGTACCCTAGTTATTAATAGTAATCA 
               
               
                 ATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA 
               
               
                 TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC 
               
               
                 CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAAC 
               
               
                 TGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG 
               
               
                 ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGG 
               
               
                 CAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTT 
               
               
                 CACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTT 
               
               
                 TGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGG 
               
               
                 CGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGC 
               
               
                 TCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGC 
               
               
                 GCGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCG 
               
               
                 CGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCC 
               
               
                 CTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC 
               
               
                 GTGAAAGCCTTGAGGGGCTCCGGGAGCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCT 
               
               
                 TTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAA 
               
               
                 TTCTAGCGGCCGCCACCATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAG 
               
               
                 ATTGGGGCAGGAGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAG 
               
               
                 GTCCAGGTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACA 
               
               
                 TCAAGCCTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCT 
               
               
                 CAACGAGGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACG 
               
               
                 TATACGGGCTCCATCCTGGTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGA 
               
               
                 GCACATCCGCCAGTATACCAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATT 
               
               
                 GCTGACAACTGCTACTTCAACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTG 
               
               
                 GGGAATCTGGGGCCGGGAAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCA 
               
               
                 TCAGTGGGCAGCACTCGTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGC 
               
               
                 ATTTGGGAATGCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGAC 
               
               
                 ATCCACTTCAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAA 
               
               
                 AAGTCACGTGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGC 
               
               
                 TGGAGGGTATGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACA 
               
               
                 ACTACTTGGCCATGGGTAACTGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGC 
               
               
                 CAACATCCGCTCCGCCATGAAGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCG 
               
               
                 AAGCTCCTGGCTGCCATCCTGCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAA 
               
               
                 ACCTGGATGCCTGTGAGGTTCTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAG 
               
               
                 GTGAACCCCCCAGACCTGATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGA 
               
               
                 CGGTGTCCACCCCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGG 
               
               
                 GATCTACGGGCGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCT 
               
               
                 CCCTCCCAGGATGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGA 
               
               
                 GAACTTTGCTGTGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGC 
               
               
                 AGTTCTTTGTGCGGCACGTGTTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGA 
               
               
                 CTGGCTGCACATCGAGTTCACTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCC 
               
               
                 ATGAACATCATCTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCA 
               
               
                 TGTTACACAAGCTGAACTCCCAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAA 
               
               
                 CCATGAGACCCAGTTTGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCT 
               
               
                 TCCTGGAGAAGAACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAG 
               
               
                 GAACAAGTTCATCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAG 
               
               
                 CGCTCGCCCACACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGG 
               
               
                 GTGCCTGCCAGCCCTTCTTTGTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTG 
               
               
                 TTCGACCGGCACCTGTGCGTGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAA 
               
               
                 TCCGCCGAGCTGGCTACCCCATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTG 
               
               
                 CTGCTGCCAGGTGTGAAGCCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCA 
               
               
                 TGGCTGAGGCTGTGCTGGGCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCT 
               
               
                 GAAGGACCACCATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGT 
               
               
                 CATCCTCCTTCAGAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAG 
               
               
                 AACGCTGCCACACTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGG 
               
               
                 CTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGC 
               
               
                 AGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTG 
               
               
                 CGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCA 
               
               
                 TGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGA 
               
               
                 GAAAATGCGGCTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCA 
               
               
                 AGGAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTG 
               
               
                 AGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATG 
               
               
                 GAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCC 
               
               
                 TGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCT 
               
               
                 GGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGA 
               
               
                 CGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTCCAG 
               
               
                 GGGACAACCACGCACTCCTACACCCGGCGGCCACTCAAACAGCCACTGCTCTACCATGACG 
               
               
                 ACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGA 
               
               
                 CCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATG 
               
               
                 ACCAAGATTTATGAGACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAG 
               
               
                 GGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTG 
               
               
                 GTGCATTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGCAT 
               
               
                 GACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACCTCCAACCTG 
               
               
                 GAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAGCACTCCGGGACGAGATCT 
               
               
                 ACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAAGAGCAGCTATGCCCGGGGCTG 
               
               
                 GATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGTACCTGC 
               
               
                 GGAACTTCGCTAGCGGGCACTAGTCCGTCGACTGTTAATTAAGCATGCTGGGGAGAGATCT 
               
               
                 AGGAAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGC 
               
               
                 CGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCG 
               
               
                 AGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 2 is the nucleotide sequence of the first generation back-half vector of the 
               
               
                 overlap system (i.e., AAV-hMYO7A-CTlong.HA; “hMyo7a coding overlap vector B”); 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCAGATCTGGCGCGCCCAATTGGCTTCGAATTCTAGCGGCCGCTGCTTAA 
               
               
                 GCAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCCAGAGGCACTGGCGGGG 
               
               
                 TCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTG 
               
               
                 CACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCC 
               
               
                 AGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTC 
               
               
                 ACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTG 
               
               
                 AGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGGAAGAGAAGCTTCGGA 
               
               
                 AGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTG 
               
               
                 GCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGG 
               
               
                 AAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGAC 
               
               
                 ATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCC 
               
               
                 AGGCACCTAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACC 
               
               
                 TGGATGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGC 
               
               
                 CAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACCCGGCGGCCACTC 
               
               
                 AAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGA 
               
               
                 TCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGA 
               
               
                 TGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTGGGCAAGAAGACGTAC 
               
               
                 AAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCAGAAG 
               
               
                 AAGAGCAGTGTGAGGCACAAGCTGGTGCATTTGACTCTGAAAAAGAAGTCCAAGCTCACA 
               
               
                 GAGGAGGTGACCAAGAGGCTGCATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTG 
               
               
                 GAGGACCGGCCCACCTCCAACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGC 
               
               
                 GGCCAGCACTCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTC 
               
               
                 CAAGAGCAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCT 
               
               
                 CCGAGAAGTTTGTCAAGTACCTGCGGAACTTCATCCACGGGGGCCCGCCCGGCTACGCCCC 
               
               
                 GTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTCAATGGGACACGGACACAGCCGCCCAGC 
               
               
                 TGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCAATCATGTTGCCCGTGACATTCATGG 
               
               
                 ATGGGACCACCAAGACCCTGCTGACGGACTCGGCAACCACGGCCAAGGAGCTCTGCAACG 
               
               
                 CGCTGGCCGACAAGATCTCTCTCAAGGACCGGTTCGGGTTCTCCCTCTACATTGCCCTGTTT 
               
               
                 GACAAGGTGTCCTCCCTGGGCAGCGGCAGTGACCACGTCATGGACGCCATCTCCCAGTGCG 
               
               
                 AGCAGTACGCCAAGGAGCAGGGCGCCCAGGAGCGCAACGCCCCCTGGAGGCTCTTCTTCCG 
               
               
                 CAAAGAGGTCTTCACGCCCTGGCACAGCCCCTCCGAGGACAACGTGGCCACCAACCTCATC 
               
               
                 TACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGAGAAGGAGGACGAC 
               
               
                 CTGGCTGAGCTGGCCTCCCAGCAGTACTTTGTAGACTATGGCTCTGAGATGATCCTGGAGCG 
               
               
                 CCTCCTGAACCTCGTGCCCACCTACATCCCCGACCGCGAGATCACGCCCCTGAAGACGCTG 
               
               
                 GAGAAGTGGGCCCAGCTGGCCATCGCCGCCCACAAGAAGGGGATTTATGCCCAGAGGAGA 
               
               
                 ACTGATGCCCAGAAGGTCAAAGAGGATGTGGTCAGTTATGCCCGCTTCAAGTGGCCCTTGC 
               
               
                 TCTTCTCCAGGTTTTATGAAGCCTACAAATTCTCAGGCCCCAGTCTCCCCAAGAACGACGTC 
               
               
                 ATCGTGGCCGTCAACTGGACGGGTGTGTACTTTGTGGATGAGCAGGAGCAGGTACTTCTGG 
               
               
                 AGCTGTCCTTCCCAGAGATCATGGCCGTGTCCAGCAGCAGGGGAGCGAAAACGACGGCCCC 
               
               
                 CAGCTTCACGCTGGCCACCATCAAGGGGGACGAATACACCTTCACCTCCAGCAATGCTGAG 
               
               
                 GACATTCGTGACCTGGTGGTCACCTTCCTAGAGGGGCTCCGGAAGAGATCTAAGTATGTTG 
               
               
                 TGGCCCTGCAGGATAACCCCAACCCCGCAGGCGAGGAGTCAGGCTTCCTCAGCTTTGCCAA 
               
               
                 GGGAGACCTCATCATCCTGGACCATGACACGGGCGAGCAGGTCATGAACTCGGGCTGGGCC 
               
               
                 AACGGCATCAATGAGAGGACCAAGCAGCGTGGGGACTTCCCCACCGACAGTGTGTACGTCA 
               
               
                 TGCCCACTGTCACCATGCCACCGCGGGAGATTGTGGCCCTGGTCACCATGACTCCCGATCA 
               
               
                 GAGGCAGGACGTTGTCCGGCTCTTGCAGCTGCGAACGGCGGAGCCCGAGGTGCGTGCCAAG 
               
               
                 CCCTACACGCTGGAGGAGTTTTCCTATGACTACTTCAGGCCCCCACCCAAGCACACGCTGA 
               
               
                 GCCGTGTCATGGTGTCCAAGGCCCGAGGCAAGGACCGGCTGTGGAGCCACACGCGGGAAC 
               
               
                 CGCTCAAGCAGGCGCTGCTCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGCAGGAGGCCTG 
               
               
                 CCTGGCCTTCATTGCTGTGCTCAAGTACATGGGCGACTACCCGTCCAAGAGGACACGCTCC 
               
               
                 GTCAACGAGCTCACCGACCAGATCTTTGAGGGTCCCCTGAAAGCCGAGCCCCTGAAGGACG 
               
               
                 AGGCATATGTGCAGATCCTGAAGCAGCTGACCGACAACCACATCAGGTACAGCGAGGAGC 
               
               
                 GGGGTTGGGAGCTGCTCTGGCTGTGCACGGGCCTTTTCCCACCCAGCAACATCCTCCTGCCC 
               
               
                 CACGTGCAGCGCTTCCTGCAGTCCCGAAAGCACTGCCCACTCGCCATCGACTGCCTGCAAC 
               
               
                 GGCTCCAGAAAGCCCTGAGAAACGGGTCCCGGAAGTACCCTCCGCACCTGGTGGAGGTGG 
               
               
                 AGGCCATCCAGCACAAGACCACCCAGATTTTCCACAAAGTCTACTTCCCTGATGACACTGA 
               
               
                 CGAGGCCTTCGAAGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCACC 
               
               
                 AGGCTGCTCCTCAAGTCCTCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGACAAGGTCAT 
               
               
                 CAGCGTTCCTGAGAATGACTTCTTCTTTGACTTTGTTCGACACTTGACAGACTGGATAAAGA 
               
               
                 AAGCTCGGCCCATCAAGGACGGAATTGTGCCCTCACTCACCTACCAGGTGTTCTTCATGAA 
               
               
                 GAAGCTGTGGACCACCACGGTGCCAGGGAAGGATCCCATGGCCGATTCCATCTTCCACTAT 
               
               
                 TACCAGGAGTTGCCCAAGTATCTCCGAGGCTACCACAAGTGCACGCGGGAGGAGGTGCTGC 
               
               
                 AGCTGGGGGCGCTGATCTACAGGGTCAAGTTCGAGGAGGACAAGTCCTACTTCCCCAGCAT 
               
               
                 CCCCAAGCTGCTGCGGGAGCTGGTGCCCCAGGACCTTATCCGGCAGGTCTCACCTGATGAC 
               
               
                 TGGAAGCGGTCCATCGTCGCCTACTTCAACAAGCACGCAGGGAAGTCCAAGGAGGAGGCC 
               
               
                 AAGCTGGCCTTCCTGAAGCTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTCGAGGT 
               
               
                 GAAGCAAACTACGGAGCCAAACTTCCCTGAGATCCTCCTAATTGCCATCAACAAGTATGGG 
               
               
                 GTCAGCCTCATCGATCCCAAAACGAAGGATATCCTCACCACTCATCCCTTCACCAAGATCTC 
               
               
                 CAACTGGAGCAGCGGCAACACCTACTTCCACATCACCATTGGGAACTTGGTGCGCGGGAGC 
               
               
                 AAACTGCTCTGCGAGACGTCACTGGGCTACAAGATGGATGACCTCCTGACTTCCTACATTA 
               
               
                 GCCAGATGCTCACAGCCATGAGCAAACAGCGGGGCTCCAGGAGCGGCAAGTACCCTTACG 
               
               
                 ATGTACCGGATTACGCATGAGGTACCAAGGGCGAATTCTGCAGTCGACTAGAGCTCGCTGA 
               
               
                 TCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCC 
               
               
                 TTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCA 
               
               
                 TTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAG 
               
               
                 GATTGGGAAGACAATAGCAGGCATGCTGGGGAGAGATCTGAGGACTAGTCCGTCGACTGTT 
               
               
                 AATTAAGCATGCTGGGGAGAGATCTAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGC 
               
               
                 GCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCG 
               
               
                 GGCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 3 is the nucleotide sequence of the front-half vector of the exon 23/24 hybrid 
               
               
                 vector system (i.e., AAV-smCBA-hMYO7ANT-APSD-ApHead); 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCAATTCGGTACCCTAGTTATTAATAGTAATCA 
               
               
                 ATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA 
               
               
                 TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC 
               
               
                 CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAAC 
               
               
                 TGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG 
               
               
                 ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGG 
               
               
                 CAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTT 
               
               
                 CACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTT 
               
               
                 TGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGG 
               
               
                 CGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGC 
               
               
                 TCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGC 
               
               
                 GCGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCG 
               
               
                 CGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCC 
               
               
                 CTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC 
               
               
                 GTGAAAGCCTTGAGGGGCTCCGGGAGCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCT 
               
               
                 TTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAA 
               
               
                 TTCTAGCGGCCGCCACCATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAG 
               
               
                 ATTGGGGCAGGAGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAG 
               
               
                 GTCCAGGTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACA 
               
               
                 TCAAGCCTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCT 
               
               
                 CAACGAGGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACG 
               
               
                 TATACGGGCTCCATCCTGGTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGA 
               
               
                 GCACATCCGCCAGTATACCAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATT 
               
               
                 GCTGACAACTGCTACTTCAACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTG 
               
               
                 GGGAATCTGGGGCCGGGAAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCA 
               
               
                 TCAGTGGGCAGCACTCGTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGC 
               
               
                 ATTTGGGAATGCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGAC 
               
               
                 ATCCACTTCAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAA 
               
               
                 AAGTCACGTGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGC 
               
               
                 TGGAGGGTATGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACA 
               
               
                 ACTACTTGGCCATGGGTAACTGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGC 
               
               
                 CAACATCCGCTCCGCCATGAAGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCG 
               
               
                 AAGCTCCTGGCTGCCATCCTGCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAA 
               
               
                 ACCTGGATGCCTGTGAGGTTCTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAG 
               
               
                 GTGAACCCCCCAGACCTGATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGA 
               
               
                 CGGTGTCCACCCCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGG 
               
               
                 GATCTACGGGCGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCT 
               
               
                 CCCTCCCAGGATGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGA 
               
               
                 GAACTTTGCTGTGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGC 
               
               
                 AGTTCTTTGTGCGGCACGTGTTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGA 
               
               
                 CTGGCTGCACATCGAGTTCACTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCC 
               
               
                 ATGAACATCATCTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCA 
               
               
                 TGTTACACAAGCTGAACTCCCAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAA 
               
               
                 CCATGAGACCCAGTTTGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCT 
               
               
                 TCCTGGAGAAGAACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAG 
               
               
                 GAACAAGTTCATCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAG 
               
               
                 CGCTCGCCCACACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGG 
               
               
                 GTGCCTGCCAGCCCTTCTTTGTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTG 
               
               
                 TTCGACCGGCACCTGTGCGTGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAA 
               
               
                 TCCGCCGAGCTGGCTACCCCATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTG 
               
               
                 CTGCTGCCAGGTGTGAAGCCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCA 
               
               
                 TGGCTGAGGCTGTGCTGGGCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCT 
               
               
                 GAAGGACCACCATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGT 
               
               
                 CATCCTCCTTCAGAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAG 
               
               
                 AACGCTGCCACACTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGG 
               
               
                 CTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGC 
               
               
                 AGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTG 
               
               
                 CGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCA 
               
               
                 TGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGA 
               
               
                 GAAAATGCGGCTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCA 
               
               
                 AGGAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTG 
               
               
                 AGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATG 
               
               
                 GAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCC 
               
               
                 TGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGTAAG 
               
               
                 TATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAG 
               
               
                 AAGACTCTTGCGTTTCTGAGCTAGCCCCCGGGTGCGCGGCGTCGGTGGTGCCGGCGGGGGG 
               
               
                 CGCCAGGTCGCAGGCGGTGTAGGGCTCCAGGCAGGCGGCGAAGGCCATGACGTGCGCTAT 
               
               
                 GAAGGTCTGCTCCTGCACGCCGTGAACCAGGTGCGCCTGCGGGCCGCGCGCGAACACCGCC 
               
               
                 ACGTCCTCGCCTGCGTGGGTCTCTTCGTCCAGGGGCACTGCTGACTGCTGCCGATACTCGGG 
               
               
                 GCTCCCGCTCTCGCTCTCGGTAACATCCGGCCGGGCGCCGTCCTTGAGCACATAGCCTGGAC 
               
               
                 CGTTTCGTCGACTGTTAATTAAGCATGCTGGGGAGAGATCTAGGAACCCCTAGTGATGGAG 
               
               
                 TTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCG 
               
               
                 ACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 67 (only the N-myosin7A portion of SEQ ID NO: 3 (AAV-smCBA- 
               
               
                 hMYO7ANT-APSD-ApHead)) 
               
               
                 ATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAGATTGGGGCAGGAGTTCG 
               
               
                 ACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAGGTCCAGGTGGTGGATGA 
               
               
                 TGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACATCAAGCCTATGCACCCC 
               
               
                 ACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCTCAACGAGGCGGGCATCT 
               
               
                 TGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACGTATACGGGCTCCATCCTG 
               
               
                 GTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGAGCACATCCGCCAGTATAC 
               
               
                 CAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATTGCTGACAACTGCTACTTC 
               
               
                 AACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTGGGGAATCTGGGGCCGGG 
               
               
                 AAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCATCAGTGGGCAGCACTCGT 
               
               
                 GGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGCATTTGGGAATGCCAAGAC 
               
               
                 CATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGACATCCACTTCAACAAGCGG 
               
               
                 GGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAAAAGTCACGTGTCTGTCGCC 
               
               
                 AGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGCTGGAGGGTATGAGTGAGGA 
               
               
                 TCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACAACTACTTGGCCATGGGTAAC 
               
               
                 TGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGCCAACATCCGCTCCGCCATGA 
               
               
                 AGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCGAAGCTCCTGGCTGCCATCCT 
               
               
                 GCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAAACCTGGATGCCTGTGAGGTT 
               
               
                 CTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAGGTGAACCCCCCAGACCTGAT 
               
               
                 GAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGACGGTGTCCACCCCACTGAGC 
               
               
                 AGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGGGATCTACGGGCGGCTGTTCG 
               
               
                 TGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCTCCCTCCCAGGATGTGAAGAA 
               
               
                 CTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGAGAACTTTGCTGTGAACAGCT 
               
               
                 TTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGCAGTTCTTTGTGCGGCACGTG 
               
               
                 TTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGACTGGCTGCACATCGAGTTCA 
               
               
                 CTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCCATGAACATCATCTCCCTCAT 
               
               
                 CGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCATGTTACACAAGCTGAACTCC 
               
               
                 CAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAACCATGAGACCCAGTTTGGCA 
               
               
                 TCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCTTCCTGGAGAAGAACCGAGA 
               
               
                 CACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAGGAACAAGTTCATCAAGCAG 
               
               
                 ATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAGCGCTCGCCCACACTTAGCA 
               
               
                 GCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGGGTGCCTGCCAGCCCTTCTTT 
               
               
                 GTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTGTTCGACCGGCACCTGTGCG 
               
               
                 TGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAATCCGCCGAGCTGGCTACCC 
               
               
                 CATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTGCTGCTGCCAGGTGTGAAG 
               
               
                 CCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCATGGCTGAGGCTGTGCTGG 
               
               
                 GCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCTGAAGGACCACCATGACAT 
               
               
                 GCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGTCATCCTCCTTCAGAAAGTC 
               
               
                 ATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCC 
               
               
                 AGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCT 
               
               
                 GCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAG 
               
               
                 CGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCG 
               
               
                 CCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCAC 
               
               
                 CAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGG 
               
               
                 AAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAAG 
               
               
                 CATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAAG 
               
               
                 GAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGCCT 
               
               
                 GTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGC 
               
               
                 CAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAG 
               
               
                   
               
               
                 SEQ ID NO: 68 (only the N-myosin7A portion encoded by SEQ ID NO: 3 (AAV- 
               
               
                 smCBA-hMYO7ANT-APSD-APhead)) 
               
               
                 MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPT 
               
               
                 SVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIG 
               
               
                 EMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATP 
               
               
                 ILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCML 
               
               
                 EGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKL 
               
               
                 LAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLS 
               
               
                 REQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQL 
               
               
                 CINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPK 
               
               
                 GTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVH 
               
               
                 SSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLF 
               
               
                 DRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAE 
               
               
                 AVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLI 
               
               
                 QRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHR 
               
               
                 LWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKH 
               
               
                 QERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQ 
               
               
                 EGQAPSGFE 
               
               
                   
               
               
                 SEQ ID NO: 69 (Alkaline phosphatase head sequence (APhead)(e.g., AAV- 
               
               
                 smCBA-hMYO7A-NT-APSD-APhead, AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead, 
               
               
                 AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead CMv1, AAV-APhead-APSA- 
               
               
                 hMYO7ACTex22.HA-MIN, AAV-APhead-APSA-hMYO7ACTex22-MIN)) 
               
               
                 CCCCGGGTGCGCGGCGTCGGTGGTGCCGGCGGGGGGCGCCAGGTCGCAGGCGGTGTAGGG 
               
               
                 CTCCAGGCAGGCGGCGAAGGCCATGACGTGCGCTATGAAGGTCTGCTCCTGCACGCCGTGA 
               
               
                 ACCAGGTGCGCCTGCGGGCCGCGCGCGAACACCGCCACGTCCTCGCCTGCGTGGGTCTCTT 
               
               
                 CGTCCAGGGGCACTGCTGACTGCTGCCGATACTCGGGGCTCCCGCTCTCGCTCTCGGTAACA 
               
               
                 TCCGGCCGGGCGCCGTCCTTGAGCACATAGCCTGGACCGTTTC 
               
               
                   
               
               
                 SEQ ID NO: 70 Alkaline phosphatase head sequence (APhead)(e.g., AAV- 
               
               
                 smCB A-hMYO7A-NT-ex21-APSD-APhead-CMv2, AAV-smCBA-hMYO7A-NT-ex21-APSD- 
               
               
                 APhead-CMv3, AAV-APhead-APSA-hMYO7ACT-ex22-APSD-CMv2.HA, AAV-APhead- 
               
               
                 APSA-hMYO7ACT-ex22-APSD-CMv2.HA-MIN, AAV-APhead-APSA-hMYO7ACT-ex22- 
               
               
                 APSD-CMv2-MIN) 
               
               
                 CCCCGGGTGCGCGGCGTCGGTGGTGCCGGCGGGGGGCGCCAGGTCGCAGGCGGTGTAGGG 
               
               
                 CTCCAGGCAGGCGGCGAAGGCCATGACGTGCGCTATGAAGGTCTGCTCCTGCACGCCGTGA 
               
               
                 ACCAGGTGCGCCTGCGGGCCGCGCGCGAACACCGCCACGTCCTCGCCTGCGTGGGTCTCTT 
               
               
                 CGTCCAGGGGCACTGCGCACTGCTGCCGATACTCGGGGCTCCCGCTCTCGCTCTCGGTAACA 
               
               
                 TCCGGCCGGGCGCCGTCCTTGAGCACATAGCCTGGACCGTTTC 
               
               
                   
               
               
                 SEQ ID NO: 4 is the nucleotide sequence of the back-half vector of the exon 23/24 hybrid 
               
               
                 vector system (i.e., AAV-APhead-APSA-hMYO7ACT.HA); 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCAATTGGCTTCGAATTCTAGCGGCCGCCCCCG 
               
               
                 GGTGCGCGGCGTCGGTGGTGCCGGCGGGGGGCGCCAGGTCGCAGGCGGTGTAGGGCTCCA 
               
               
                 GGCAGGCGGCGAAGGCCATGACGTGCGCTATGAAGGTCTGCTCCTGCACGCCGTGAACCAG 
               
               
                 GTGCGCCTGCGGGCCGCGCGCGAACACCGCCACGTCCTCGCCTGCGTGGGTCTCTTCGTCCA 
               
               
                 GGGGCACTGCTGACTGCTGCCGATACTCGGGGCTCCCGCTCTCGCTCTCGGTAACATCCGGC 
               
               
                 CGGGCGCCGTCCTTGAGCACATAGCCTGGACCGTTTCCTTAAGCGACGCATGCTCGCGATA 
               
               
                 GGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGACCTGGAGCGAGGG 
               
               
                 CGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACGAGGATGAG 
               
               
                 GAGGACCTCTCTGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCA 
               
               
                 CGCACTCCTACACCCGGCGGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGA 
               
               
                 CCAGCTGGCAGCCCTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGC 
               
               
                 CCAAGTACCACACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTA 
               
               
                 TGAGACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGA 
               
               
                 GGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTGCATTTGAC 
               
               
                 TCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGCATGACGGGGAGTC 
               
               
                 CACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACCTCCAACCTGGAGAAGCTGCA 
               
               
                 CTTCATCATCGGCAATGGCATCCTGCGGCCAGCACTCCGGGACGAGATCTACTGCCAGATC 
               
               
                 AGCAAGCAGCTGACCCACAACCCCTCCAAGAGCAGCTATGCCCGGGGCTGGATTCTCGTGT 
               
               
                 CTCTCTGCGTGGGCTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGTACCTGCGGAACTTCATC 
               
               
                 CACGGGGGCCCGCCCGGCTACGCCCCGTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTCA 
               
               
                 ATGGGACACGGACACAGCCGCCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGC 
               
               
                 CAATCATGTTGCCCGTGACATTCATGGATGGGACCACCAAGACCCTGCTGACGGACTCGGC 
               
               
                 AACCACGGCCAAGGAGCTCTGCAACGCGCTGGCCGACAAGATCTCTCTCAAGGACCGGTTC 
               
               
                 GGGTTCTCCCTCTACATTGCCCTGTTTGACAAGGTGTCCTCCCTGGGCAGCGGCAGTGACCA 
               
               
                 CGTCATGGACGCCATCTCCCAGTGCGAGCAGTACGCCAAGGAGCAGGGCGCCCAGGAGCG 
               
               
                 CAACGCCCCCTGGAGGCTCTTCTTCCGCAAAGAGGTCTTCACGCCCTGGCACAGCCCCTCCG 
               
               
                 AGGACAACGTGGCCACCAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGA 
               
               
                 GTACAGGTGTGAGAAGGAGGACGACCTGGCTGAGCTGGCCTCCCAGCAGTACTTTGTAGAC 
               
               
                 TATGGCTCTGAGATGATCCTGGAGCGCCTCCTGAACCTCGTGCCCACCTACATCCCCGACCG 
               
               
                 CGAGATCACGCCCCTGAAGACGCTGGAGAAGTGGGCCCAGCTGGCCATCGCCGCCCACAA 
               
               
                 GAAGGGGATTTATGCCCAGAGGAGAACTGATGCCCAGAAGGTCAAAGAGGATGTGGTCAG 
               
               
                 TTATGCCCGCTTCAAGTGGCCCTTGCTCTTCTCCAGGTTTTATGAAGCCTACAAATTCTCAG 
               
               
                 GCCCCAGTCTCCCCAAGAACGACGTCATCGTGGCCGTCAACTGGACGGGTGTGTACTTTGT 
               
               
                 GGATGAGCAGGAGCAGGTACTTCTGGAGCTGTCCTTCCCAGAGATCATGGCCGTGTCCAGC 
               
               
                 AGCAGGGGAGCGAAAACGACGGCCCCCAGCTTCACGCTGGCCACCATCAAGGGGGACGAA 
               
               
                 TACACCTTCACCTCCAGCAATGCTGAGGACATTCGTGACCTGGTGGTCACCTTCCTAGAGGG 
               
               
                 GCTCCGGAAGAGATCTAAGTATGTTGTGGCCCTGCAGGATAACCCCAACCCCGCAGGCGAG 
               
               
                 GAGTCAGGCTTCCTCAGCTTTGCCAAGGGAGACCTCATCATCCTGGACCATGACACGGGCG 
               
               
                 AGCAGGTCATGAACTCGGGCTGGGCCAACGGCATCAATGAGAGGACCAAGCAGCGTGGGG 
               
               
                 ACTTCCCCACCGACAGTGTGTACGTCATGCCCACTGTCACCATGCCACCGCGGGAGATTGTG 
               
               
                 GCCCTGGTCACCATGACTCCCGATCAGAGGCAGGACGTTGTCCGGCTCTTGCAGCTGCGAA 
               
               
                 CGGCGGAGCCCGAGGTGCGTGCCAAGCCCTACACGCTGGAGGAGTTTTCCTATGACTACTT 
               
               
                 CAGGCCCCCACCCAAGCACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGAGGCAAGGAC 
               
               
                 CGGCTGTGGAGCCACACGCGGGAACCGCTCAAGCAGGCGCTGCTCAAGAAGCTCCTGGGC 
               
               
                 AGTGAGGAGCTCTCGCAGGAGGCCTGCCTGGCCTTCATTGCTGTGCTCAAGTACATGGGCG 
               
               
                 ACTACCCGTCCAAGAGGACACGCTCCGTCAACGAGCTCACCGACCAGATCTTTGAGGGTCC 
               
               
                 CCTGAAAGCCGAGCCCCTGAAGGACGAGGCATATGTGCAGATCCTGAAGCAGCTGACCGA 
               
               
                 CAACCACATCAGGTACAGCGAGGAGCGGGGTTGGGAGCTGCTCTGGCTGTGCACGGGCCTT 
               
               
                 TTCCCACCCAGCAACATCCTCCTGCCCCACGTGCAGCGCTTCCTGCAGTCCCGAAAGCACTG 
               
               
                 CCCACTCGCCATCGACTGCCTGCAACGGCTCCAGAAAGCCCTGAGAAACGGGTCCCGGAAG 
               
               
                 TACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAGCACAAGACCACCCAGATTTTCCACA 
               
               
                 AAGTCTACTTCCCTGATGACACTGACGAGGCCTTCGAAGTGGAGTCCAGCACCAAGGCCAA 
               
               
                 GGACTTCTGCCAGAACATCGCCACCAGGCTGCTCCTCAAGTCCTCAGAGGGATTCAGCCTCT 
               
               
                 TTGTCAAAATTGCAGACAAGGTCATCAGCGTTCCTGAGAATGACTTCTTCTTTGACTTTGTT 
               
               
                 CGACACTTGACAGACTGGATAAAGAAAGCTCGGCCCATCAAGGACGGAATTGTGCCCTCAC 
               
               
                 TCACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGACCACCACGGTGCCAGGGAAGGATCC 
               
               
                 CATGGCCGATTCCATCTTCCACTATTACCAGGAGTTGCCCAAGTATCTCCGAGGCTACCACA 
               
               
                 AGTGCACGCGGGAGGAGGTGCTGCAGCTGGGGGCGCTGATCTACAGGGTCAAGTTCGAGG 
               
               
                 AGGACAAGTCCTACTTCCCCAGCATCCCCAAGCTGCTGCGGGAGCTGGTGCCCCAGGACCT 
               
               
                 TATCCGGCAGGTCTCACCTGATGACTGGAAGCGGTCCATCGTCGCCTACTTCAACAAGCAC 
               
               
                 GCAGGGAAGTCCAAGGAGGAGGCCAAGCTGGCCTTCCTGAAGCTCATCTTCAAGTGGCCCA 
               
               
                 CCTTTGGCTCAGCCTTCTTCGAGGTGAAGCAAACTACGGAGCCAAACTTCCCTGAGATCCTC 
               
               
                 CTAATTGCCATCAACAAGTATGGGGTCAGCCTCATCGATCCCAAAACGAAGGATATCCTCA 
               
               
                 CCACTCATCCCTTCACCAAGATCTCCAACTGGAGCAGCGGCAACACCTACTTCCACATCACC 
               
               
                 ATTGGGAACTTGGTGCGCGGGAGCAAACTGCTCTGCGAGACGTCACTGGGCTACAAGATGG 
               
               
                 ATGACCTCCTGACTTCCTACATTAGCCAGATGCTCACAGCCATGAGCAAACAGCGGGGCTC 
               
               
                 CAGGAGCGGCAAGTACCCTTACGATGTACCGGATTACGCATGAGGTACCAAGGGCGAATTC 
               
               
                 TGCAGTCGACTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTT 
               
               
                 GTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAA 
               
               
                 TAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGG 
               
               
                 TGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGAGAGA 
               
               
                 TCTGAGGACTAGTCCGTCGACTGTTAATTAAGCATGCTGGGGAGAGATCTAGGAAACCCCT 
               
               
                 AGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCA 
               
               
                 AAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGA 
               
               
                 GAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 71 myosin7A (e.g., AAV-APhead-APSA-hMYO7ACT.HA) 
               
               
                 GACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTG 
               
               
                 CCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGTTCGCGGCCACCTACT 
               
               
                 TCCAGGGGACAACCACGCACTCCTACACCCGGCGGCCACTCAAACAGCCACTGCTCTACCA 
               
               
                 TGACGACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGATCACCATCCTCCGCTTCATG 
               
               
                 GGGGACCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGATGGCAGTGAGAAGATCCCTG 
               
               
                 TGATGACCAAGATTTATGAGACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCC 
               
               
                 TGCAGGGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACA 
               
               
                 AGCTGGTGCATTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGC 
               
               
                 TGCATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACCTCCA 
               
               
                 ACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAGCACTCCGGGACGA 
               
               
                 GATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAAGAGCAGCTATGCCCGG 
               
               
                 GGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGTA 
               
               
                 CCTGCGGAACTTCATCCACGGGGGCCCGCCCGGCTACGCCCCGTACTGTGAGGAGCGCCTG 
               
               
                 AGAAGGACCTTTGTCAATGGGACACGGACACAGCCGCCCAGCTGGCTGGAGCTGCAGGCC 
               
               
                 ACCAAGTCCAAGAAGCCAATCATGTTGCCCGTGACATTCATGGATGGGACCACCAAGACCC 
               
               
                 TGCTGACGGACTCGGCAACCACGGCCAAGGAGCTCTGCAACGCGCTGGCCGACAAGATCTC 
               
               
                 TCTCAAGGACCGGTTCGGGTTCTCCCTCTACATTGCCCTGTTTGACAAGGTGTCCTCCCTGG 
               
               
                 GCAGCGGCAGTGACCACGTCATGGACGCCATCTCCCAGTGCGAGCAGTACGCCAAGGAGC 
               
               
                 AGGGCGCCCAGGAGCGCAACGCCCCCTGGAGGCTCTTCTTCCGCAAAGAGGTCTTCACGCC 
               
               
                 CTGGCACAGCCCCTCCGAGGACAACGTGGCCACCAACCTCATCTACCAGCAGGTGGTGCGA 
               
               
                 GGAGTCAAGTTTGGGGAGTACAGGTGTGAGAAGGAGGACGACCTGGCTGAGCTGGCCTCC 
               
               
                 CAGCAGTACTTTGTAGACTATGGCTCTGAGATGATCCTGGAGCGCCTCCTGAACCTCGTGCC 
               
               
                 CACCTACATCCCCGACCGCGAGATCACGCCCCTGAAGACGCTGGAGAAGTGGGCCCAGCTG 
               
               
                 GCCATCGCCGCCCACAAGAAGGGGATTTATGCCCAGAGGAGAACTGATGCCCAGAAGGTC 
               
               
                 AAAGAGGATGTGGTCAGTTATGCCCGCTTCAAGTGGCCCTTGCTCTTCTCCAGGTTTTATGA 
               
               
                 AGCCTACAAATTCTCAGGCCCCAGTCTCCCCAAGAACGACGTCATCGTGGCCGTCAACTGG 
               
               
                 ACGGGTGTGTACTTTGTGGATGAGCAGGAGCAGGTACTTCTGGAGCTGTCCTTCCCAGAGA 
               
               
                 TCATGGCCGTGTCCAGCAGCAGGGGAGCGAAAACGACGGCCCCCAGCTTCACGCTGGCCAC 
               
               
                 CATCAAGGGGGACGAATACACCTTCACCTCCAGCAATGCTGAGGACATTCGTGACCTGGTG 
               
               
                 GTCACCTTCCTAGAGGGGCTCCGGAAGAGATCTAAGTATGTTGTGGCCCTGCAGGATAACC 
               
               
                 CCAACCCCGCAGGCGAGGAGTCAGGCTTCCTCAGCTTTGCCAAGGGAGACCTCATCATCCT 
               
               
                 GGACCATGACACGGGCGAGCAGGTCATGAACTCGGGCTGGGCCAACGGCATCAATGAGAG 
               
               
                 GACCAAGCAGCGTGGGGACTTCCCCACCGACAGTGTGTACGTCATGCCCACTGTCACCATG 
               
               
                 CCACCGCGGGAGATTGTGGCCCTGGTCACCATGACTCCCGATCAGAGGCAGGACGTTGTCC 
               
               
                 GGCTCTTGCAGCTGCGAACGGCGGAGCCCGAGGTGCGTGCCAAGCCCTACACGCTGGAGGA 
               
               
                 GTTTTCCTATGACTACTTCAGGCCCCCACCCAAGCACACGCTGAGCCGTGTCATGGTGTCCA 
               
               
                 AGGCCCGAGGCAAGGACCGGCTGTGGAGCCACACGCGGGAACCGCTCAAGCAGGCGCTGC 
               
               
                 TCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGCAGGAGGCCTGCCTGGCCTTCATTGCTGT 
               
               
                 GCTCAAGTACATGGGCGACTACCCGTCCAAGAGGACACGCTCCGTCAACGAGCTCACCGAC 
               
               
                 CAGATCTTTGAGGGTCCCCTGAAAGCCGAGCCCCTGAAGGACGAGGCATATGTGCAGATCC 
               
               
                 TGAAGCAGCTGACCGACAACCACATCAGGTACAGCGAGGAGCGGGGTTGGGAGCTGCTCT 
               
               
                 GGCTGTGCACGGGCCTTTTCCCACCCAGCAACATCCTCCTGCCCCACGTGCAGCGCTTCCTG 
               
               
                 CAGTCCCGAAAGCACTGCCCACTCGCCATCGACTGCCTGCAACGGCTCCAGAAAGCCCTGA 
               
               
                 GAAACGGGTCCCGGAAGTACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAGCACAAGA 
               
               
                 CCACCCAGATTTTCCACAAAGTCTACTTCCCTGATGACACTGACGAGGCCTTCGAAGTGGA 
               
               
                 GTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCACCAGGCTGCTCCTCAAGTCC 
               
               
                 TCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGACAAGGTCATCAGCGTTCCTGAGAATG 
               
               
                 ACTTCTTCTTTGACTTTGTTCGACACTTGACAGACTGGATAAAGAAAGCTCGGCCCATCAAG 
               
               
                 GACGGAATTGTGCCCTCACTCACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGACCACCA 
               
               
                 CGGTGCCAGGGAAGGATCCCATGGCCGATTCCATCTTCCACTATTACCAGGAGTTGCCCAA 
               
               
                 GTATCTCCGAGGCTACCACAAGTGCACGCGGGAGGAGGTGCTGCAGCTGGGGGCGCTGATC 
               
               
                 TACAGGGTCAAGTTCGAGGAGGACAAGTCCTACTTCCCCAGCATCCCCAAGCTGCTGCGGG 
               
               
                 AGCTGGTGCCCCAGGACCTTATCCGGCAGGTCTCACCTGATGACTGGAAGCGGTCCATCGT 
               
               
                 CGCCTACTTCAACAAGCACGCAGGGAAGTCCAAGGAGGAGGCCAAGCTGGCCTTCCTGAA 
               
               
                 GCTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTCGAGGTGAAGCAAACTACGGAGC 
               
               
                 CAAACTTCCCTGAGATCCTCCTAATTGCCATCAACAAGTATGGGGTCAGCCTCATCGATCCC 
               
               
                 AAAACGAAGGATATCCTCACCACTCATCCCTTCACCAAGATCTCCAACTGGAGCAGCGGCA 
               
               
                 ACACCTACTTCCACATCACCATTGGGAACTTGGTGCGCGGGAGCAAACTGCTCTGCGAGAC 
               
               
                 GTCACTGGGCTACAAGATGGATGACCTCCTGACTTCCTACATTAGCCAGATGCTCACAGCC 
               
               
                 ATGAGCAAACAGCGGGGCTCCAGGAGCGGCAAG 
               
               
                   
               
               
                 SEQ ID NO: 72 (hemagglutinin (HA) tag (AAV-APhead-APSA-hMYO7ACT.HA, 
               
               
                 AAV-hMYO7A-CTlong.HA) 
               
               
                 TACCCTTACGATGTACCGGATTACGCATGA 
               
            
           
         
       
     
     EXAMPLE 6 shows second generation hybrid vectors that minimize expression of truncated MYO7A protein. 
     The hybrid and simple overlap front half vectors as described in Examples 1-5 contained a MYO7A cDNA sequence that encoded a portion of the MYO7A protein tail domain. The tail domain of MYO7A is known for its ability to bind other cellular proteins. In Examples 1-5 of the present application, it is shown that the original hybrid front half vector was capable of encoding a MYO7A protein ( FIGS.  28 - 30   ) (Dyka, et al. 2014). However, some loss of retinal structure/function was observed following injection of original hybrid front half vectors into mouse retina. This loss of retinal structure/function was hypothesized to be a result of the gain of function, which is exerted by the truncated MYO7A protein containing a partial tail domain. The truncated MYO7A protein was shown to be produced from only the front-half vectors ( FIGS.  31 - 32   ). As a control, the expression levels of any truncated protein encoded by the back-half vectors was also measured. These results demonstrate that back-half vectors do not produce a truncated product and do not lead to a loss of retinal structure/function ( FIG.  33   ). 
     To eliminate this gain of function toxicity associated with the truncated MYO7A protein fragment, improved, second generation hybrid and simple overlap vectors were developed with the goal of eliminating the formation of the truncated protein from the front-half vectors. Though the previously developed simple overlap vector did not produce observable quantities of truncated MYO7A protein, and did not exhibit observable loss of structure/function following its injection in mice, a second generation simple overlap vector was nevertheless developed in conjunction with the second generation hybrid vector. This was done as a precaution to hedge against the possibility that the original simple overlap front-half vector may express truncated MYO7A at levels unable to be detected via immunoblot and tolerability studies in mouse. 
     In both the hybrid and simple overlap platforms, sequence corresponding to the tail domain from the front-half vectors was moved to the back-half vectors. The MYO7A protein is an actin-based molecular motor, wherein the N-terminal (head) contains an actin-binding site and an ATP-binding site. The 5IQ (neck) is stabilized by calmodulin, and there is a single a-helix (SAH) that acts as a lever. The C-terminal (tail) domain of the MYO7A protein determines the functional specificity. Notably, in the hybrid vector, the ‘split point’ was moved from exon 23/24 in the original to exon 21/22 in the second generation ( FIGS.  34  and  50 A ), such that the second generation vector systems have the split point moved from one side of the single alpha-helix (SAH) to the other. This new split between exons 21 and 22 is positioned between the 5IQ (neck) and SAH. 
     In the simple overlap vector, the amount of overlap sequence was reduced such that no sequence corresponding to the tail domain remained in the front vector (this new vector construct will be hereinafter referred to as the “second generation overlap”). Thus, the second generation overlap vector would contain a shorter segment of overlapping sequence such that the overlap ended at the split point between exons 21 and 22. The second generation hybrid and second generation overlap vectors described herein ensure that no portion of tail domain is encoded by either the hybrid front-, or simple overlap front-half vector. The vector systems were altered in this way to reduce production of truncated MYO7A protein. 
     When the second generation hybrid vectors containing the exon 21/22 split point were administered in vitro to HEK293 cells, a truncated MYO7A protein of smaller size, corresponding to the change in vector sequence was observed ( FIGS.  35  and  39   ). When the second generation hybrid vectors containing the exon 21/22 split point were administered in vivo via subretinal injection in Myo7a −/−  mice, the smaller sized MYO7A protein was also observed ( FIGS.  36  and  38   ). The second generation hybrid vectors containing the exon 21/22 split point express equivalent amounts of full-length MYO7A compared to original hybrid vectors containing the exon 23/24 split point (see, e.g.,  FIGS.  35  and  36   ), but do not produce the MYO7A tail protein fragment observed in original hybrid vectors ( FIG.  38   ). In sum, it is demonstrated herein that the second generation hybrid and simple overlap vectors that do not express a tail domain sequence in the front half vectors result in a MYO7A protein product that is better tolerated in retina. 
     The front half AAV-MYO7A vectors (both hybrid and simple overlap) contain promoters and inverted terminal repeats (ITRs). It is possible that the ITRs provide a polyadenylation signal that, together with the promoter, leads to the production and maturation of messenger RNA for translation. In the context of the hybrid vectors, it is also possible that the alkaline phosphatase (AP) splice donor and APhead ‘intron’ sequence are also facilitating maturation of the mRNA by providing a splicing signal. While the exact mechanism promoting mRNA maturation is unclear, it is observable that hybrid front half vectors do produce truncated protein ( FIGS.  31 - 33   ). 
     Though it is demonstrated herein that the second generation hybrid vectors result in diminished production of undesired products, additional improvements were made to the vectors to reduce the production of truncated MYO7A, thereby further increasing safety and efficiency. In the hybrid vectors, which contain the second generation exon 21/22 split point as described above, potential in-frame stop codons located downstream of the MYO7A sequence were removed from the front hybrid vector plasmids ( FIG.  42   ). The single nucleotide substitutions removing these potential stop codons are designed to take advantage of the ‘non-stop’ decay mechanism of the cell, thereby eliminating the spurious RNA before it can be translated. In-frame stop codons were removed in two stages. First, three potential stop codons within the AP splice donor sequence were removed from the second generation hybrid vector to create a further improved vector still containing the exon 21/22 split point (“CMv1 hybrid”; SEQ ID NOs: 33 and 32;  FIG.  42   ). 
     An additional potential in-frame stop codon was located within the shared recombinogenic AP region. Thus, a separate, further improved vector was generated wherein there were functional improvements made in both the front-half and back-half second generation hybrid vectors containing the exon 21/22 split point (“CMv2 hybrid”; SEQ ID NOs: 34 and 35). The CMv2 hybrid front half vector has the three potential stop codons located in the AP splice donor sequence, as well as the one potential stop codon located in the APhead recombinogenic sequence, removed. The CMv2 hybrid back half vector has an identical change made in the APhead recombinogenic sequence so as to match the front half vector. 
     Upon making the modifications that result in the CMv2 hybrid back-half vector as described herein, the CMv2 hybrid back-half vector becomes close to exceeding, but does not actually exceed, the packaging limit of an AAV vector construct. To mitigate the possibility that the back-half vector is too large, a construct was designed that modifies the CMv2 hybrid back-half vector to remove any extraneous sequence existing between elements in the construct, as well as the HA sequence. Once modified, the resulting back-half vector is designated as the CMv2.1 hybrid back-half vector (SEQ ID NO: 44). 
     The CMv1 and CMv2 hybrid vectors were tested in vitro, by transfecting these plasmids into HEK293 cells. The first generation hybrid front half vector (“original”) and the second generation hybrid front half vector (“ex21/22”) were also used for comparison ( FIG.  40   ). It was found that the CMv1 hybrid front half vector still produced truncated MYO7A. However, the CMv2 hybrid front half vector did not produce any truncated protein. Vinculin (VCL) was used as a loading control in all samples. 
     Corresponding changes were made in the simple overlap vector, despite the absence of truncated protein encoded by this vector ( FIG.  43   ). Because the putative stop codons that were removed in the CMv1 and CMv2 hybrid vectors do not exist in the front-half of the simple overlap vectors, slightly different modifications were made to the simple overlap vectors. In the CMv1 overlap vector, the simple overlap vector has been modified in the 3′ untranslated region, between the MYO7A partial coding sequence and the 3′ AAV ITR, to remove potential in-frame stop codons (“CMv1 overlap”; SEQ ID NOs: 36 and 38;  FIG.  43   ). 
     As shown in  FIG.  41   , AAV-mediated MYO7A transcript is expressed in macaque retina, and tolerability of dual AAV5-MYO7A vectors in subretinally injected macaque retinas is shown. Macaque retinas were evaluated 2 months after injection. 
     These data show great promise for application of the technology to human patients. 
     EXAMPLE 7 provides third generation hybrid vectors. 
     Third Generation Overlap Vectors 
     An improved third generation (V3) overlap vector pair was generated by altering the overlapping regions of the MYO7A coding sequence. This V3 overlap pair consists of a front half vector (“AAV-smCBA-hMYO7A-NTlong-v3”) comprising the nucleotide sequence of SEQ ID NO: 50, and a back half vector (“AAV-smCBA-hMYO7A-CTlong-v3.HA”) comprising the nucleotide sequence of SEQ ID NO: 51. This V3 overlap pair contains an N-terminal myosin 7A coding sequence comprising SEQ ID NO: 66 and a C-terminal myosin 7A coding sequence comprising SEQ ID NO: 80. These vectors contain shortened overlapping region length (see  FIGS.  46  and  47   ), such as overlapping region lengths of 945 bp and 687 bp. Reduction of the length of the overlapping region has the effect of reducing the size of the vector genome overall. 
     The V3 Overlap dual vector system was shown to produce increased levels of full-length MYO7A protein as compared to the original vectors, as quantified by the Protein Simple Jess system, as shown in  FIGS.  48 A and  49 A . Overlap vectors containing 687 bp and 945 bp of overlapping MYO7A sequence provided optimal expression levels. 
     As shown in  FIG.  49 B , the V3 overlap vectors do not produce an appreciable amount of undesired truncated MYO7A fragment when their overlapping region length remains above 361 bp. Thus, reducing overlap length to a certain point, and therefore ensuring neither vector genome is pushing the packaging capacity of AAV capsid (4.7 to 4.9 kb), leads to increased expression of full length MYO7A. If overlap length is too small (&lt;361 bp), full length MYO7A expression is reduced, and truncated protein may appear. 
     By virtue of the shorter length of their overlapping regions, these improved, third generation overlap vectors contain shorter ITR to ITR (ITR-ITR) lengths (see  FIG.  48 B ). In particular embodiments, the length between the inverted terminal repeats at each end of the first AAV vector polynucleotide is about 4615 nucleotides (nt) or fewer. The ITR-ITR length in the improved first vector polynucleotide is 4615 nt. In particular embodiments, the length between the inverted terminal repeats at each end of the second AAV vector polynucleotide is about 4800 nt or fewer. The ITR-ITR length in the improved second vector polynucleotide is about 4560 nt. 
     hMYO7A overlapping regions, e.g., SEQ ID NOs: 39 and 53-59, may be used as the polynucleotide sequence that overlaps in additional overlap dual vectors expressing large genes (other than MYO7A). In particular, disclosed herein are overlap dual vectors that express a large gene other than MYO7A and that comprises a nucleotide sequence having at least 80%, 85%, 90%, 95%, 98%, or 99% identity to any one of SEQ ID NOs: 39 and 52-59. In particular embodiments, these overlap dual vectors comprises the nucleotide sequence of any one of SEQ ID NOs: 39 and 53-59, e.g., SEQ ID NO: 56 or 57, and express a large gene selected from ABCA4, CEP290, EYS, RP1, ALMS1, CDH23, PCDH15, USH1C, USH1G, USH2A, DNFB31, DMD, CFTR, GDE, DYSF, F8, and DFNB2. In some embodiments, these overlap vectors contain two overlapping sequences disclosed herein, e.g., the mutually exclusive sequences SEQ ID NOs: 39 and 56, or the mutually exclusive sequences SEQ ID NOs: 39 and 57. rAAV virions containing V3 overlap vector pairs containing 687 bp and 945 bp of overlapping region length are packaged and administered to retinal cells. rAAV virions containing V3 overlap vector pairs containing 687 bp and 945 bp of overlapping region length are packaged and administered to auditory hair cells. 
     Overlap vectors containing the following pairs of myosin7A-encoding nucleotide sequences are evaluated in their abilities to produce full-length MYO7A polypeptide, in vitro or in vivo. In vitro evaluation may be performed using Protein Simple Jess Western blotting.
         the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 63, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 83;   the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 63, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 90;   the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 101, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 83;   the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 101, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 90;   the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 66, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 83; and   the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 66, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 90.       

     Accordingly, in some embodiments, provided herein are polynucleotide vector systems in which the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the SEQ ID NO: 63, and the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to nucleotide sequence of SEQ ID NO: 83. In some embodiments, the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 90. 
     In some embodiments, the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 101. In some embodiments, the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 66. In some embodiments, provided herein are polynucleotide vector systems in which the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the SEQ ID NO: 66, and the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to nucleotide sequence of SEQ ID NO: 90. 
     The following overlap vectors are evaluated in their abilities to produce full-length MYO7A polypeptide, in vitro or in vivo. In vitro evaluation may be performed using Protein Simple Jess Western blotting.
         the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 50, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 51;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 50, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 38;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 1, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 38;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 50, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 2;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 1, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 51;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 36, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 2;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 36, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 38;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 36, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 51;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 37, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 38; and   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 37, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 51.       

     Accordingly, in some embodiments, provided herein are polynucleotide vector systems in which the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the SEQ ID NO: 50, and the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to nucleotide sequence of SEQ ID NO: 51. In some embodiments, the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 38. In some embodiments, the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 2. 
     In some embodiments, the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 1. In some embodiments, the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 36. In some embodiments, the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 37. In some embodiments, provided herein are polynucleotide vector systems in which the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the SEQ ID NO: 37, and the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to nucleotide sequence of SEQ ID NO: 38 or SEQ ID NO: 51. 
     Third Generation Hybrid Vectors 
     An improved third generation (CMv3) hybrid system, or vector pair, was generated by making substitutions into three putative stop codons in the 3′ untranslated region (UTR) of the front-half vector of the CMv2 hybrid system. As such, the CMv3 Hybrid Front Half vector contains substitutions in (i.e., removal of) one in-frame stop codon in the APhead sequence, three in-frame stop codons in the AP intron sequence, and three in-frame stop codons in the 3′ UTR sequence. In exemplary embodiments, the CMv3 hybrid system is a CMv3 MIN system as residual, unseeded legacy sequences (such as restriction enzyme sites) have been removed. 
     Exemplary CMv3 hybrid systems consist of i) the front half vector (“AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead-CMv3”) comprising the nucleotide sequence of SEQ ID NO: 46, and ii) a CMv2 back half vector (“AAV-APhead-APSA-ex22hMYO7A-CT.HA-CMv2)”) comprising the nucleotide sequence of SEQ ID NO: 35, a CMv2.1 back half vector (“AAV-APhead-APSA-hMYO7ACTex22-CMv2.1”) comprising the nucleotide sequence of SEQ ID NO: 44, or a minimized version of either vector, e.g. a CMv2 (or V2-)Back MIN comprising the nucleotide sequence of SEQ ID NO: 49. 
     To generate the Hybrid-CMv3 back MIN and Hybrid-CMv2 back MIN vectors, an ‘unneeded legacy’ sequence was removed from the back half vector to ensure the vector size did not exceed the packaging capacity of an AAV capsid. By virtue of the removal of unseeded legacy sequences from the back, these improved, third generation hybrid vectors contain shorter ITR to ITR (ITR-ITR) lengths (see  FIGS.  50 C and  51 B ). In particular embodiments, the length between the inverted terminal repeats at each end of a first AAV vector polynucleotide of the hybrid vectors is about 4279 nucleotides (nt) or fewer. This has the effect of minimizing the length of the back half vector, which improved packaging efficiency. The Hybrid-V2 back MIN is 122 bp smaller (4981 vs. 4859 bp) than the associated original vector, and the Hybrid_CMv2 back MIN is 121 bp smaller (4982 vs. 4861 bp) than the associated original vector. For the Hybrid-V2 back MIN HA and Hybrid-CMV2 back MIN HA vectors, a hemagglutinin (HA) tag was added to the minimized back half vectors (Hybrid-V2 back MIN and Hybrid-CMV2 back MIN) to allow for detection in normal monkeys. 
     The CMv3 Hybrid vector system was shown to produce comparably low levels of the truncated MYO7A fragment as compared to Hybrid CMv1 and Hybrid CMv2 vectors, as shown in  FIG.  50 D . Further, as shown in  FIGS.  51 C and  51 D , the pairing the hybrid CMv3 front half vector with a CMv3 MIN back half vector produced full-length MYO7A at levels equal to or above that seen with the original (1 st  generation) hybrid vectors. This pair of vectors produced comparably low levels of truncated fragment, as shown in  FIG.  51 E . 
     The inventors have also discovered that wherein a hMYO7A sequence may be used as the intronic sequence mediating recombination in the cell following administration in hybrid dual vectors expressing large genes (other than MYO7A). Such hybrid vectors are generated to contain one or more overlapping regions identified through improvement of the MYO7A overlap vectors provided herein. (Such vectors may or do not contain an APhead sequence and/or an AP intronic sequence, to allow for the insertion of one of these overlapping regions.) In particular, disclosed herein are hybrid dual vectors that contain a sequence between the first intron and second intron of the first and second AAV vector polynucleotides, respectively, that comprises a nucleotide sequence having at least 80%, 85%, 90%, 95%, 98%, or 99% identity to any one of SEQ ID NOs: 39 and 52-59. In particular embodiments, hybrid dual vectors contain a sequence between the first intron and second intron of the first and second AAV vector polynucleotides, respectively, that comprises the nucleotide sequence of any one of SEQ ID NOs: 39 and 53-59, e.g., SEQ ID NO: 56 or 57. Additional large genes that may be delivered with these hybrid vectors include ABCA4, CEP290, EYS, RP1, ALMS1, CDH23, PCDH15, USH1C, USH1G, USH2A, DNFB31, DMD, CFTR, GDE, DYSF, F8, and DFNB2. In some embodiments, these hybrid dual vectors contain an intron sequence containing two overlapping sequences disclosed herein, e.g., the mutually exclusive sequences SEQ ID NOs: 39 and 56, or the mutually exclusive sequences SEQ ID NOs: 39 and 57. 
     rAAV virions containing CMv3 hybrid vector pairs comprising the SEQ ID NO: 46 front-half vector and SEQ ID NO: 35 back-half vector are packaged and administered to retinal cells. rAAV virions containing CMv3 hybrid vector pairs comprising the SEQ ID NO: 46 front-half vector and SEQ ID NO: 35 back-half vector are packaged and administered to auditory hair cells. 
     Hybrid vectors containing the following pair of myosin7A-encoding nucleotide sequences are evaluated in their abilities to produce full-length MYO7A polypeptide, in vitro or in vivo:
         the first AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 73, and the second AAV vector polynucleotide comprises the nucleotide sequence of SEQ ID NO: 75.       

     Accordingly, in some embodiments, provided herein are polynucleotide vector systems in which the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the SEQ ID NO: 73, and the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to nucleotide sequence of SEQ ID NO: 75. 
     Hybrid vector systems comprising the following pairs of vector sequences are evaluated in their abilities to produce full-length MYO7A polypeptide, in vitro or in vivo.
         the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 31, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 32;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 46, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 35;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 46, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 49;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 34, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 47;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 31, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 48;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 31, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 49;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 33, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 32;   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 34, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 35; and   the first AAV vector comprises the nucleotide sequence of SEQ ID NO: 34, and the second AAV vector comprises the nucleotide sequence of SEQ ID NO: 44.       

     Accordingly, in some embodiments, provided herein are polynucleotide vector systems in which the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the SEQ ID NO: 31, and the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to nucleotide sequence of SEQ ID NO: 32. In some embodiments, the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 48 or SEQ ID NO: 49. In some embodiments, the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 33. 
     In some embodiments, provided herein are polynucleotide vector systems in which the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the SEQ ID NO: 46, and the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to nucleotide sequence of SEQ ID NO: 35. In some embodiments, the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 49. In some embodiments, the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 34. 
     In some embodiments, provided herein are polynucleotide vector systems in which the first AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to the SEQ ID NO: 34, and the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to nucleotide sequence of SEQ ID NO: 47. In some embodiments, the second AAV vector polynucleotide comprises a nucleotide sequence that is at least about 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% identical to SEQ ID NO: 35 or 44. 
     The polynucleotide vector systems of the disclosure may comprise a nucleotide sequence that is at least 80%, 85%, 90%, 92.5%, 95%, 96%, 97%, 98%, 99%, or 99.5% identical to any of the following sequences (e.g., any of SEQ ID NOs: 31-38, 44, and 46-51). In some embodiments, the vectors comprise a sequence comprising any one of SEQ ID NOs: 31-38, 44, and 46-51. In some embodiments, the vector systems of the disclosure comprise a nucleotide sequence that contains that differs from any of the sequences of SEQ ID NOs: 31-38, 44, and 46-51 by 1, 2, 3, 4, 5, 5-10, 10-15, 15-20, 20-25, 25-30, 30-35, 35-40, 40-45, 45-50, or more than 50 nucleotides. The disclosed vectors may differ in any of the following vector sequences in the presence, or absence, of a tag such as an HA tag. The disclosed vectors may contain stretches of 5-10, 10-15, 15-20, 20-25, 25-35, 35-45, 45-60, 60-75 or more than 75 consecutive nucleotides in common with any of SEQ ID NOs: 31-38, 44, and 46-51. 
     The myosin7a-encoding sequences of any of the polynucleotide vectors provided herein may comprise a nucleotide sequence that is at least 80%, 85%, 90%, 92.5%, 95%, 96%, 97%, 98%, 99%, or 99.5% identical to any of the hMyosin7a-encoding sequences in the N-terminal (front half, “—NT”) or C-terminal (back half, “—CT”) vectors that follow, e.g., SEQ ID NOs: 63, 66, 73, 75, 77, 80, and 90. The disclosed N-terminal and C-terminal myosin7a-encoding sequences may contain stretches of 5-10, 10-15, 15-20, 20-25, 25-35, 35-45, 45-60, 60-75 or more than 75 consecutive nucleotides in common with any of SEQ ID NOs: 63, 66, 73, 75, 77, 80, and 90. 
     The polynucleotide vectors and myosin7a-encoding sequences provided herein may comprise a nucleotide sequence that differs from any of the following sequences (e.g., any of the vectors of SEQ ID NOs: 31-38, 44, and 46-51; or any of the hMyosin7a-encoding sequences in the N-terminal (front half, “—NT”) or C-terminal (back half, “—CT”) vectors set forth as SEQ ID NOs: 63, 66, 73, 75, 77, 80, and 90) by 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 10-15, 15-20, 20-25, or more than 25 nucleotides. The polynucleotide vectors provided herein may comprise a nucleotide sequence that differs from any of the following sequences by 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more than 10 nucleotides at or near the 5′ terminus of the vector; and may differ from any of these sequences by 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more than 10 nucleotides at or near the 3′ terminus. The vectors provided herein may comprise truncations by 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides at the 5′ or 3′ terminus. 
     Nucleotide Sequences of the Vectors Provided in Examples 6 and 7 
       
     
       
         
           
               
               
            
               
                 SEQ ID NO: 31 is the nucleotide sequence of the second generation hybrid front-half 
                   
               
               
                 vector (i.e., AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead): 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCAATTCGGTACCCTAGTTATTAATAGTAATCA 
               
               
                 ATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA 
               
               
                 TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC 
               
               
                 CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAAC 
               
               
                 TGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG 
               
               
                 ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGG 
               
               
                 CAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTT 
               
               
                 CACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTT 
               
               
                 TGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGG 
               
               
                 CGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGC 
               
               
                 TCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGC 
               
               
                 GCGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCG 
               
               
                 CGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCC 
               
               
                 CTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC 
               
               
                 GTGAAAGCCTTGAGGGGCTCCGGGAGCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCT 
               
               
                 TTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAA 
               
               
                 TTCTAGCGGCCGCCACCATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAG 
               
               
                 ATTGGGGCAGGAGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAG 
               
               
                 GTCCAGGTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACA 
               
               
                 TCAAGCCTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCT 
               
               
                 CAACGAGGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACG 
               
               
                 TATACGGGCTCCATCCTGGTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGA 
               
               
                 GCACATCCGCCAGTATACCAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATT 
               
               
                 GCTGACAACTGCTACTTCAACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTG 
               
               
                 GGGAATCTGGGGCCGGGAAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCA 
               
               
                 TCAGTGGGCAGCACTCGTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGC 
               
               
                 ATTTGGGAATGCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGAC 
               
               
                 ATCCACTTCAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAA 
               
               
                 AAGTCACGTGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGC 
               
               
                 TGGAGGGTATGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACA 
               
               
                 ACTACTTGGCCATGGGTAACTGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGC 
               
               
                 CAACATCCGCTCCGCCATGAAGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCG 
               
               
                 AAGCTCCTGGCTGCCATCCTGCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAA 
               
               
                 ACCTGGATGCCTGTGAGGTTCTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAG 
               
               
                 GTGAACCCCCCAGACCTGATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGA 
               
               
                 CGGTGTCCACCCCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGG 
               
               
                 GATCTACGGGCGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCT 
               
               
                 CCCTCCCAGGATGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGA 
               
               
                 GAACTTTGCTGTGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGC 
               
               
                 AGTTCTTTGTGCGGCACGTGTTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGA 
               
               
                 CTGGCTGCACATCGAGTTCACTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCC 
               
               
                 ATGAACATCATCTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCA 
               
               
                 TGTTACACAAGCTGAACTCCCAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAA 
               
               
                 CCATGAGACCCAGTTTGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCT 
               
               
                 TCCTGGAGAAGAACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAG 
               
               
                 GAACAAGTTCATCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAG 
               
               
                 CGCTCGCCCACACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGG 
               
               
                 GTGCCTGCCAGCCCTTCTTTGTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTG 
               
               
                 TTCGACCGGCACCTGTGCGTGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAA 
               
               
                 TCCGCCGAGCTGGCTACCCCATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTG 
               
               
                 CTGCTGCCAGGTGTGAAGCCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCA 
               
               
                 TGGCTGAGGCTGTGCTGGGCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCT 
               
               
                 GAAGGACCACCATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGT 
               
               
                 CATCCTCCTTCAGAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAG 
               
               
                 AACGCTGCCACACTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGG 
               
               
                 CTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGC 
               
               
                 AGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTG 
               
               
                 CGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCA 
               
               
                 TGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGAGGTAAGTATCAAGGTTACAAGACA 
               
               
                 GGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGA 
               
               
                 GCTAGCCCCCGGGTGCGCGGCGTCGGTGGTGCCGGCGGGGGGCGCCAGGTCGCAGGCGGT 
               
               
                 GTAGGGCTCCAGGCAGGCGGCGAAGGCCATGACGTGCGCTATGAAGGTCTGCTCCTGCACG 
               
               
                 CCGTGAACCAGGTGCGCCTGCGGGCCGCGCGCGAACACCGCCACGTCCTCGCCTGCGTGGG 
               
               
                 TCTCTTCGTCCAGGGGCACTGCTGACTGCTGCCGATACTCGGGGCTCCCGCTCTCGCTCTCG 
               
               
                 GTAACATCCGGCCGGGCGCCGTCCTTGAGCACATAGCCTGGACCGTTTCGTCGACTGTTAAT 
               
               
                 TAAGCATGCTGGGGAGAGATCTAGGAAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTG 
               
               
                 CGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCC 
               
               
                 GGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 73 (hMYO7A-NT (AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead), 
               
               
                 (AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead CMv1), (AAV-smCBA-hMYO7A-NT- 
               
               
                 Ex21-APSD-APhead CMv2), and (AAV-smCBA-hMY07A- NT-Ex21-APSD-APhead-CMv3)) 
               
               
                 ATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAGATTGGGGCAGGAGTTCG 
               
               
                 ACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAGGTCCAGGTGGTGGATGA 
               
               
                 TGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACATCAAGCCTATGCACCCC 
               
               
                 ACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCTCAACGAGGCGGGCATCT 
               
               
                 TGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACGTATACGGGCTCCATCCTG 
               
               
                 GTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGAGCACATCCGCCAGTATAC 
               
               
                 CAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATTGCTGACAACTGCTACTTC 
               
               
                 AACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTGGGGAATCTGGGGCCGGG 
               
               
                 AAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCATCAGTGGGCAGCACTCGT 
               
               
                 GGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGCATTTGGGAATGCCAAGAC 
               
               
                 CATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGACATCCACTTCAACAAGCGG 
               
               
                 GGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAAAAGTCACGTGTCTGTCGCC 
               
               
                 AGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGCTGGAGGGTATGAGTGAGGA 
               
               
                 TCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACAACTACTTGGCCATGGGTAAC 
               
               
                 TGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGCCAACATCCGCTCCGCCATGA 
               
               
                 AGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCGAAGCTCCTGGCTGCCATCCT 
               
               
                 GCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAAACCTGGATGCCTGTGAGGTT 
               
               
                 CTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAGGTGAACCCCCCAGACCTGAT 
               
               
                 GAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGACGGTGTCCACCCCACTGAGC 
               
               
                 AGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGGGATCTACGGGCGGCTGTTCG 
               
               
                 TGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCTCCCTCCCAGGATGTGAAGAA 
               
               
                 CTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGAGAACTTTGCTGTGAACAGCT 
               
               
                 TTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGCAGTTCTTTGTGCGGCACGTG 
               
               
                 TTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGACTGGCTGCACATCGAGTTCA 
               
               
                 CTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCCATGAACATCATCTCCCTCAT 
               
               
                 CGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCATGTTACACAAGCTGAACTCC 
               
               
                 CAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAACCATGAGACCCAGTTTGGCA 
               
               
                 TCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCTTCCTGGAGAAGAACCGAGA 
               
               
                 CACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAGGAACAAGTTCATCAAGCAG 
               
               
                 ATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAGCGCTCGCCCACACTTAGCA 
               
               
                 GCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGGGTGCCTGCCAGCCCTTCTTT 
               
               
                 GTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTGTTCGACCGGCACCTGTGCG 
               
               
                 TGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAATCCGCCGAGCTGGCTACCC 
               
               
                 CATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTGCTGCTGCCAGGTGTGAAG 
               
               
                 CCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCATGGCTGAGGCTGTGCTGG 
               
               
                 GCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCTGAAGGACCACCATGACAT 
               
               
                 GCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGTCATCCTCCTTCAGAAAGTC 
               
               
                 ATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCC 
               
               
                 AGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCT 
               
               
                 GCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAG 
               
               
                 CGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCG 
               
               
                 CCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCAC 
               
               
                 CAACGCCTCAGGGCTGAG 
               
               
                   
               
               
                 SEQ ID NO: 74 (hMYO7A-NT (AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead), 
               
               
                 (AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead CMvl), (AAV-smCBA-hMY07A- NT- 
               
               
                 Ex21-APSD-APhead-CMv3)) 
               
               
                 MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPT 
               
               
                 SVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIG 
               
               
                 EMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATP 
               
               
                 ILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCML 
               
               
                 EGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKL 
               
               
                 LAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLS 
               
               
                 REQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQL 
               
               
                 CINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPK 
               
               
                 GTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVH 
               
               
                 SSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLF 
               
               
                 DRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAE 
               
               
                 AVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLI 
               
               
                 QRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHR 
               
               
                 LWAVLTVQAYARGMIARRLHQRLRAE 
               
               
                   
               
               
                 SEQ ID NO: 32 is the nucleotide sequence of the second generation hybrid back-half 
               
               
                 vector (i.e., AAV-APhead-APSA-ex22hMYO7A-CT.HA (with hemagglutinin (HA) tag)): 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCAATTGGCTTCGAATTCTAGCGGCCGCCCCCG 
               
               
                 GGTGCGCGGCGTCGGTGGTGCCGGCGGGGGGCGCCAGGTCGCAGGCGGTGTAGGGCTCCA 
               
               
                 GGCAGGCGGCGAAGGCCATGACGTGCGCTATGAAGGTCTGCTCCTGCACGCCGTGAACCAG 
               
               
                 GTGCGCCTGCGGGCCGCGCGCGAACACCGCCACGTCCTCGCCTGCGTGGGTCTCTTCGTCCA 
               
               
                 GGGGCACTGCTGACTGCTGCCGATACTCGGGGCTCCCGCTCTCGCTCTCGGTAACATCCGGC 
               
               
                 CGGGCGCCGTCCTTGAGCACATAGCCTGGACCGTTTCCTTAAGCGACGCATGCTCGCGATA 
               
               
                 GGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGTATCTGTGGCGCCTCG 
               
               
                 AGGCTGAGAAAATGCGGCTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAG 
               
               
                 AAGGCCAAGGAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAG 
               
               
                 GACGCTGAGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAG 
               
               
                 CAGATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGATGTTTG 
               
               
                 GCTTCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGA 
               
               
                 GGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCT 
               
               
                 GCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGTTCGCGGCCACCTAC 
               
               
                 TTCCAGGGGACAACCACGCACTCCTACACCCGGCGGCCACTCAAACAGCCACTGCTCTACC 
               
               
                 ATGACGACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGATCACCATCCTCCGCTTCAT 
               
               
                 GGGGGACCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGATGGCAGTGAGAAGATCCCT 
               
               
                 GTGATGACCAAGATTTATGAGACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCC 
               
               
                 CTGCAGGGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCAC 
               
               
                 AAGCTGGTGCATTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGG 
               
               
                 CTGCATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACCTCC 
               
               
                 AACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAGCACTCCGGGACG 
               
               
                 AGATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAAGAGCAGCTATGCCCG 
               
               
                 GGGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGT 
               
               
                 ACCTGCGGAACTTCATCCACGGGGGCCCGCCCGGCTACGCCCCGTACTGTGAGGAGCGCCT 
               
               
                 GAGAAGGACCTTTGTCAATGGGACACGGACACAGCCGCCCAGCTGGCTGGAGCTGCAGGC 
               
               
                 CACCAAGTCCAAGAAGCCAATCATGTTGCCCGTGACATTCATGGATGGGACCACCAAGACC 
               
               
                 CTGCTGACGGACTCGGCAACCACGGCCAAGGAGCTCTGCAACGCGCTGGCCGACAAGATCT 
               
               
                 CTCTCAAGGACCGGTTCGGGTTCTCCCTCTACATTGCCCTGTTTGACAAGGTGTCCTCCCTG 
               
               
                 GGCAGCGGCAGTGACCACGTCATGGACGCCATCTCCCAGTGCGAGCAGTACGCCAAGGAG 
               
               
                 CAGGGCGCCCAGGAGCGCAACGCCCCCTGGAGGCTCTTCTTCCGCAAAGAGGTCTTCACGC 
               
               
                 CCTGGCACAGCCCCTCCGAGGACAACGTGGCCACCAACCTCATCTACCAGCAGGTGGTGCG 
               
               
                 AGGAGTCAAGTTTGGGGAGTACAGGTGTGAGAAGGAGGACGACCTGGCTGAGCTGGCCTC 
               
               
                 CCAGCAGTACTTTGTAGACTATGGCTCTGAGATGATCCTGGAGCGCCTCCTGAACCTCGTGC 
               
               
                 CCACCTACATCCCCGACCGCGAGATCACGCCCCTGAAGACGCTGGAGAAGTGGGCCCAGCT 
               
               
                 GGCCATCGCCGCCCACAAGAAGGGGATTTATGCCCAGAGGAGAACTGATGCCCAGAAGGT 
               
               
                 CAAAGAGGATGTGGTCAGTTATGCCCGCTTCAAGTGGCCCTTGCTCTTCTCCAGGTTTTATG 
               
               
                 AAGCCTACAAATTCTCAGGCCCCAGTCTCCCCAAGAACGACGTCATCGTGGCCGTCAACTG 
               
               
                 GACGGGTGTGTACTTTGTGGATGAGCAGGAGCAGGTACTTCTGGAGCTGTCCTTCCCAGAG 
               
               
                 ATCATGGCCGTGTCCAGCAGCAGGGGAGCGAAAACGACGGCCCCCAGCTTCACGCTGGCCA 
               
               
                 CCATCAAGGGGGACGAATACACCTTCACCTCCAGCAATGCTGAGGACATTCGTGACCTGGT 
               
               
                 GGTCACCTTCCTAGAGGGGCTCCGGAAGAGATCTAAGTATGTTGTGGCCCTGCAGGATAAC 
               
               
                 CCCAACCCCGCAGGCGAGGAGTCAGGCTTCCTCAGCTTTGCCAAGGGAGACCTCATCATCC 
               
               
                 TGGACCATGACACGGGCGAGCAGGTCATGAACTCGGGCTGGGCCAACGGCATCAATGAGA 
               
               
                 GGACCAAGCAGCGTGGGGACTTCCCCACCGACAGTGTGTACGTCATGCCCACTGTCACCAT 
               
               
                 GCCACCGCGGGAGATTGTGGCCCTGGTCACCATGACTCCCGATCAGAGGCAGGACGTTGTC 
               
               
                 CGGCTCTTGCAGCTGCGAACGGCGGAGCCCGAGGTGCGTGCCAAGCCCTACACGCTGGAGG 
               
               
                 AGTTTTCCTATGACTACTTCAGGCCCCCACCCAAGCACACGCTGAGCCGTGTCATGGTGTCC 
               
               
                 AAGGCCCGAGGCAAGGACCGGCTGTGGAGCCACACGCGGGAACCGCTCAAGCAGGCGCTG 
               
               
                 CTCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGCAGGAGGCCTGCCTGGCCTTCATTGCTG 
               
               
                 TGCTCAAGTACATGGGCGACTACCCGTCCAAGAGGACACGCTCCGTCAACGAGCTCACCGA 
               
               
                 CCAGATCTTTGAGGGTCCCCTGAAAGCCGAGCCCCTGAAGGACGAGGCATATGTGCAGATC 
               
               
                 CTGAAGCAGCTGACCGACAACCACATCAGGTACAGCGAGGAGCGGGGTTGGGAGCTGCTC 
               
               
                 TGGCTGTGCACGGGCCTTTTCCCACCCAGCAACATCCTCCTGCCCCACGTGCAGCGCTTCCT 
               
               
                 GCAGTCCCGAAAGCACTGCCCACTCGCCATCGACTGCCTGCAACGGCTCCAGAAAGCCCTG 
               
               
                 AGAAACGGGTCCCGGAAGTACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAGCACAAG 
               
               
                 ACCACCCAGATTTTCCACAAAGTCTACTTCCCTGATGACACTGACGAGGCCTTCGAAGTGG 
               
               
                 AGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCACCAGGCTGCTCCTCAAGTC 
               
               
                 CTCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGACAAGGTCATCAGCGTTCCTGAGAAT 
               
               
                 GACTTCTTCTTTGACTTTGTTCGACACTTGACAGACTGGATAAAGAAAGCTCGGCCCATCAA 
               
               
                 GGACGGAATTGTGCCCTCACTCACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGACCACC 
               
               
                 ACGGTGCCAGGGAAGGATCCCATGGCCGATTCCATCTTCCACTATTACCAGGAGTTGCCCA 
               
               
                 AGTATCTCCGAGGCTACCACAAGTGCACGCGGGAGGAGGTGCTGCAGCTGGGGGCGCTGAT 
               
               
                 CTACAGGGTCAAGTTCGAGGAGGACAAGTCCTACTTCCCCAGCATCCCCAAGCTGCTGCGG 
               
               
                 GAGCTGGTGCCCCAGGACCTTATCCGGCAGGTCTCACCTGATGACTGGAAGCGGTCCATCG 
               
               
                 TCGCCTACTTCAACAAGCACGCAGGGAAGTCCAAGGAGGAGGCCAAGCTGGCCTTCCTGAA 
               
               
                 GCTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTCGAGGTGAAGCAAACTACGGAGC 
               
               
                 CAAACTTCCCTGAGATCCTCCTAATTGCCATCAACAAGTATGGGGTCAGCCTCATCGATCCC 
               
               
                 AAAACGAAGGATATCCTCACCACTCATCCCTTCACCAAGATCTCCAACTGGAGCAGCGGCA 
               
               
                 ACACCTACTTCCACATCACCATTGGGAACTTGGTGCGCGGGAGCAAACTGCTCTGCGAGAC 
               
               
                 GTCACTGGGCTACAAGATGGATGACCTCCTGACTTCCTACATTAGCCAGATGCTCACAGCC 
               
               
                 ATGAGCAAACAGCGGGGCTCCAGGAGCGGCAAGTACCCTTACGATGTACCGGATTACGCAT 
               
               
                 GAGGTACCAAGGGCGAATTCTGCAGTCGACTAGAGCTCGCTGATCAGCCTCGACTGTGCCT 
               
               
                 TCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCC 
               
               
                 ACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCA 
               
               
                 TTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAG 
               
               
                 CAGGCATGCTGGGGAGAGATCTGGAGGACTAGTCCGTCGACTGTTAATTAAGCATGCTGGG 
               
               
                 GAGAGATCTAGGAAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCT 
               
               
                 CACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTG 
               
               
                 AGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 75, ex22hMYO7A (e.g., AAV-APhead-APSA-ex22hMYO7A-CT.HA, 
               
               
                 AAV-APhead-APSA-ex22hMYO7A -CT.HA-CMv2, AAV-APhead-APSA-hMYO7ACTex22- 
               
               
                 CMv2.1.HA, AAV-APhead-APSA-hMYO7ACTex22-CMv2, AAV-APhead-APSA- 
               
               
                 hMYO7ACTex 22.HA-MIN, and AAV-APhead-APSA-hMYO7ACTex 22-MIN)) 
               
               
                 TATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGGAAGAGAAGCTTCGGAAG 
               
               
                 GAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCC 
               
               
                 CAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAG 
               
               
                 AAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGG 
               
               
                 TGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGC 
               
               
                 ACCTAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGA 
               
               
                 TGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGT 
               
               
                 TCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACCCGGCGGCCACTCAAACA 
               
               
                 GCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGATCACC 
               
               
                 ATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGATGGCA 
               
               
                 GTGAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTGGGCAAGAAGACGTACAAGAG 
               
               
                 GGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAG 
               
               
                 CAGTGTGAGGCACAAGCTGGTGCATTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAG 
               
               
                 GTGACCAAGAGGCTGCATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGAC 
               
               
                 CGGCCCACCTCCAACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAG 
               
               
                 CACTCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAAGAG 
               
               
                 CAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCTCCGAGA 
               
               
                 AGTTTGTCAAGTACCTGCGGAACTTCATCCACGGGGGCCCGCCCGGCTACGCCCCGTACTGT 
               
               
                 GAGGAGCGCCTGAGAAGGACCTTTGTCAATGGGACACGGACACAGCCGCCCAGCTGGCTGG 
               
               
                 AGCTGCAGGCCACCAAGTCCAAGAAGCCAATCATGTTGCCCGTGACATTCATGGATGGGAC 
               
               
                 CACCAAGACCCTGCTGACGGACTCGGCAACCACGGCCAAGGAGCTCTGCAACGCGCTGGCC 
               
               
                 GACAAGATCTCTCTCAAGGACCGGTTCGGGTTCTCCCTCTACATTGCCCTGTTTGACAAGGT 
               
               
                 GTCCTCCCTGGGCAGCGGCAGTGACCACGTCATGGACGCCATCTCCCAGTGCGAGCAGTAC 
               
               
                 GCCAAGGAGCAGGGCGCCCAGGAGCGCAACGCCCCCTGGAGGCTCTTCTTCCGCAAAGAGG 
               
               
                 TCTTCACGCCCTGGCACAGCCCCTCCGAGGACAACGTGGCCACCAACCTCATCTACCAGCAG 
               
               
                 GTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGAGAAGGAGGACGACCTGGCTGAG 
               
               
                 CTGGCCTCCCAGCAGTACTTTGTAGACTATGGCTCTGAGATGATCCTGGAGCGCCTCCTGAA 
               
               
                 CCTCGTGCCCACCTACATCCCCGACCGCGAGATCACGCCCCTGAAGACGCTGGAGAAGTGG 
               
               
                 GCCCAGCTGGCCATCGCCGCCCACAAGAAGGGGATTTATGCCCAGAGGAGAACTGATGCCC 
               
               
                 AGAAGGTCAAAGAGGATGTGGTCAGTTATGCCCGCTTCAAGTGGCCCTTGCTCTTCTCCAGG 
               
               
                 TTTTATGAAGCCTACAAATTCTCAGGCCCCAGTCTCCCCAAGAACGACGTCATCGTGGCCGT 
               
               
                 CAACTGGACGGGTGTGTACTTTGTGGATGAGCAGGAGCAGGTACTTCTGGAGCTGTCCTTCC 
               
               
                 CAGAGATCATGGCCGTGTCCAGCAGCAGGGGAGCGAAAACGACGGCCCCCAGCTTCACGCT 
               
               
                 GGCCACCATCAAGGGGGACGAATACACCTTCACCTCCAGCAATGCTGAGGACATTCGTGAC 
               
               
                 CTGGTGGTCACCTTCCTAGAGGGGCTCCGGAAGAGATCTAAGTATGTTGTGGCCCTGCAGG 
               
               
                 ATAACCCCAACCCCGCAGGCGAGGAGTCAGGCTTCCTCAGCTTTGCCAAGGGAGACCTCAT 
               
               
                 CATCCTGGACCATGACACGGGCGAGCAGGTCATGAACTCGGGCTGGGCCAACGGCATCAAT 
               
               
                 GAGAGGACCAAGCAGCGTGGGGACTTCCCCACCGACAGTGTGTACGTCATGCCCACTGTCA 
               
               
                 CCATGCCACCGCGGGAGATTGTGGCCCTGGTCACCATGACTCCCGATCAGAGGCAGGACGT 
               
               
                 TGTCCGGCTCTTGCAGCTGCGAACGGCGGAGCCCGAGGTGCGTGCCAAGCCCTACACGCTG 
               
               
                 GAGGAGTTTTCCTATGACTACTTCAGGCCCCCACCCAAGCACACGCTGAGCCGTGTCATGGT 
               
               
                 GTCCAAGGCCCGAGGCAAGGACCGGCTGTGGAGCCACACGCGGGAACCGCTCAAGCAGGC 
               
               
                 GCTGCTCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGCAGGAGGCCTGCCTGGCCTTCATT 
               
               
                 GCTGTGCTCAAGTACATGGGCGACTACCCGTCCAAGAGGACACGCTCCGTCAACGAGCTCA 
               
               
                 CCGACCAGATCTTTGAGGGTCCCCTGAAAGCCGAGCCCCTGAAGGACGAGGCATATGTGCA 
               
               
                 GATCCTGAAGCAGCTGACCGACAACCACATCAGGTACAGCGAGGAGCGGGGTTGGGAGCT 
               
               
                 GCTCTGGCTGTGCACGGGCCTTTTCCCACCCAGCAACATCCTCCTGCCCCACGTGCAGCGCT 
               
               
                 TCCTGCAGTCCCGAAAGCACTGCCCACTCGCCATCGACTGCCTGCAACGGCTCCAGAAAGC 
               
               
                 CCTGAGAAACGGGTCCCGGAAGTACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAGCAC 
               
               
                 AAGACCACCCAGATTTTCCACAAAGTCTACTTCCCTGATGACACTGACGAGGCCTTCGAAGT 
               
               
                 GGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCACCAGGCTGCTCCTCAAG 
               
               
                 TCCTCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGACAAGGTCATCAGCGTTCCTGAGAA 
               
               
                 TGACTTCTTCTTTGACTTTGTTCGACACTTGACAGACTGGATAAAGAAAGCTCGGCCCATCA 
               
               
                 AGGACGGAATTGTGCCCTCACTCACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGACCACC 
               
               
                 ACGGTGCCAGGGAAGGATCCCATGGCCGATTCCATCTTCCACTATTACCAGGAGTTGCCCAA 
               
               
                 GTATCTCCGAGGCTACCACAAGTGCACGCGGGAGGAGGTGCTGCAGCTGGGGGCGCTGATC 
               
               
                 TACAGGGTCAAGTTCGAGGAGGACAAGTCCTACTTCCCCAGCATCCCCAAGCTGCTGCGGG 
               
               
                 AGCTGGTGCCCCAGGACCTTATCCGGCAGGTCTCACCTGATGACTGGAAGCGGTCCATCGTC 
               
               
                 GCCTACTTCAACAAGCACGCAGGGAAGTCCAAGGAGGAGGCCAAGCTGGCCTTCCTGAAGC 
               
               
                 TCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTCGAGGTGAAGCAAACTACGGAGCCA 
               
               
                 AACTTCCCTGAGATCCTCCTAATTGCCATCAACAAGTATGGGGTCAGCCTCATCGATCCCAA 
               
               
                 AACGAAGGATATCCTCACCACTCATCCCTTCACCAAGATCTCCAACTGGAGCAGCGGCAAC 
               
               
                 ACCTACTTCCACATCACCATTGGGAACTTGGTGCGCGGGAGCAAACTGCTCTGCGAGACGTC 
               
               
                 ACTGGGCTACAAGATGGATGACCTCCTGACTTCCTACATTAGCCAGATGCTCACAGCCATGA 
               
               
                 GCAAACAGCGGGGCTCCAGGAGCGGCAAG 
               
               
                   
               
               
                 SEQ ID NO: 76, ex22hMYO7A (e.g., AAV-APhead-APSA-ex22hMYO7A-CT.HA, 
               
               
                 AAV-APhead-APSA-ex22hMYO7A -CT.HA-CMv2, and AAV-APhead-APSA- 
               
               
                 hMYO7ACTex22-CMv2.1 .HA)) 
               
               
                 YLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKE 
               
               
                 LLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAAL 
               
               
                 PLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMG 
               
               
                 DLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVH 
               
               
                 LTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQL 
               
               
                 THNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPP 
               
               
                 SWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDK 
               
               
                 VSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQV 
               
               
                 VRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAI 
               
               
                 AAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGV 
               
               
                 YFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLR 
               
               
                 KRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTD 
               
               
                 SVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHT 
               
               
                 LSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSV 
               
               
                 NELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRF 
               
               
                 LQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESST 
               
               
                 KAKDFCQNIATRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLT 
               
               
                 YQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKS 
               
               
                 YFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFE 
               
               
                 VKQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCET 
               
               
                 SLGYKMDDLLTSYISQMLTAMSKQRGSRSGK 
               
               
                   
               
               
                 SEQ ID NO: 33 is the nucleotide sequence of the CMv1 hybrid front-half vector (i.e., 
               
               
                 AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead CMv1): 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCG 
               
               
                 ACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCA 
               
               
                 TCACTAGGGGTTCTCAGATCTGGCGCGCCCAATTCGGTACCCTAGTTATTAATAGTAATCAA 
               
               
                 TTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAAT 
               
               
                 GGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCC 
               
               
                 CATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTG 
               
               
                 CCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGAC 
               
               
                 GGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCA 
               
               
                 GTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCA 
               
               
                 CTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTG 
               
               
                 TGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCG 
               
               
                 AGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCC 
               
               
                 GAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCG 
               
               
                 GCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCC 
               
               
                 GCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCT 
               
               
                 CCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAA 
               
               
                 AGCCTTGAGGGGCTCCGGGAGCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCC 
               
               
                 TACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTCTAG 
               
               
                 CGGCCGCCACCATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAGATTGGG 
               
               
                 GCAGGAGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAGGTCCAG 
               
               
                 GTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACATCAAGC 
               
               
                 CTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCTCAACGA 
               
               
                 GGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACGTATACGG 
               
               
                 GCTCCATCCTGGTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGAGCACATC 
               
               
                 CGCCAGTATACCAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATTGCTGACA 
               
               
                 ACTGCTACTTCAACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTGGGGAATC 
               
               
                 TGGGGCCGGGAAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCATCAGTGGG 
               
               
                 CAGCACTCGTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGCATTTGGGA 
               
               
                 ATGCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGACATCCACTT 
               
               
                 CAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAAAAGTCACG 
               
               
                 TGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGCTGGAGGGTA 
               
               
                 TGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACAACTACTTGGC 
               
               
                 CATGGGTAACTGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGCCAACATCCGC 
               
               
                 TCCGCCATGAAGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCGAAGCTCCTGG 
               
               
                 CTGCCATCCTGCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAAACCTGGATGC 
               
               
                 CTGTGAGGTTCTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAGGTGAACCCCC 
               
               
                 CAGACCTGATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGACGGTGTCCAC 
               
               
                 CCCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGGGATCTACGGG 
               
               
                 CGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCTCCCTCCCAGGA 
               
               
                 TGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGAGAACTTTGCTG 
               
               
                 TGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGCAGTTCTTTGTG 
               
               
                 CGGCACGTGTTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGACTGGCTGCACA 
               
               
                 TCGAGTTCACTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCCATGAACATCAT 
               
               
                 CTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCATGTTACACAAG 
               
               
                 CTGAACTCCCAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAACCATGAGACCC 
               
               
                 AGTTTGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCTTCCTGGAGAAG 
               
               
                 AACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAGGAACAAGTTCA 
               
               
                 TCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAGCGCTCGCCCAC 
               
               
                 ACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGGGTGCCTGCCAG 
               
               
                 CCCTTCTTTGTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTGTTCGACCGGCA 
               
               
                 CCTGTGCGTGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAATCCGCCGAGCT 
               
               
                 GGCTACCCCATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTGCTGCTGCCAGG 
               
               
                 TGTGAAGCCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCATGGCTGAGGCT 
               
               
                 GTGCTGGGCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCTGAAGGACCACC 
               
               
                 ATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGTCATCCTCCTTCA 
               
               
                 GAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACA 
               
               
                 CTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGG 
               
               
                 GCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCC 
               
               
                 CGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCG 
               
               
                 CCACCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGC 
               
               
                 TGCACCAACGCCTCAGGGCTGAGGTAAGTATCAAGGTTACAAGACAGGTTAACGGAGACCA 
               
               
                 ATTGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCAGCGCTAGCCCCCGGGTG 
               
               
                 CGCGGCGTCGGTGGTGCCGGCGGGGGGCGCCAGGTCGCAGGCGGTGTAGGGCTCCAGGCA 
               
               
                 GGCGGCGAAGGCCATGACGTGCGCTATGAAGGTCTGCTCCTGCACGCCGTGAACCAGGTGC 
               
               
                 GCCTGCGGGCCGCGCGCGAACACCGCCACGTCCTCGCCTGCGTGGGTCTCTTCGTCCAGGGG 
               
               
                 CACTGCTGACTGCTGCCGATACTCGGGGCTCCCGCTCTCGCTCTCGGTAACATCCGGCCGGG 
               
               
                 CGCCGTCCTTGAGCACATAGCCTGGACCGTTTCGTCGACTGTTAATTAAGCATGCTGGGGAG 
               
               
                 AGATCTGAGGAAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCA 
               
               
                 CTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAG 
               
               
                 CGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 34 is the nucleotide sequence of the CMv2 hybrid front-half vector (i.e.. 
               
               
                 AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead CMv2): 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCG 
               
               
                 ACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCA 
               
               
                 TCACTAGGGGTTCTCAGATCTGGCGCGCCCAATTCGGTACCCTAGTTATTAATAGTAATCAA 
               
               
                 TTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAAT 
               
               
                 GGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCC 
               
               
                 CATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTG 
               
               
                 CCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGAC 
               
               
                 GGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCA 
               
               
                 GTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCA 
               
               
                 CTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTG 
               
               
                 TGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCG 
               
               
                 AGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCC 
               
               
                 GAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCG 
               
               
                 GCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCC 
               
               
                 GCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCT 
               
               
                 CCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAA 
               
               
                 AGCCTTGAGGGGCTCCGGGAGCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCC 
               
               
                 TACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTCTAG 
               
               
                 CGGCCGCCACCATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAGATTGGG 
               
               
                 GCAGGAGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAGGTCCAG 
               
               
                 GTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACATCAAGC 
               
               
                 CTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCTCAACGA 
               
               
                 GGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACGTATACGG 
               
               
                 GCTCCATCCTGGTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGAGCACATC 
               
               
                 CGCCAGTATACCAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATTGCTGACA 
               
               
                 ACTGCTACTTCAACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTGGGGAATC 
               
               
                 TGGGGCCGGGAAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCATCAGTGGG 
               
               
                 CAGCACTCGTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGCATTTGGGA 
               
               
                 ATGCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGACATCCACTT 
               
               
                 CAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAAAAGTCACG 
               
               
                 TGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGCTGGAGGGTA 
               
               
                 TGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACAACTACTTGGC 
               
               
                 CATGGGTAACTGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGCCAACATCCGC 
               
               
                 TCCGCCATGAAGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCGAAGCTCCTGG 
               
               
                 CTGCCATCCTGCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAAACCTGGATGC 
               
               
                 CTGTGAGGTTCTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAGGTGAACCCCC 
               
               
                 CAGACCTGATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGACGGTGTCCAC 
               
               
                 CCCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGGGATCTACGGG 
               
               
                 CGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCTCCCTCCCAGGA 
               
               
                 TGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGAGAACTTTGCTG 
               
               
                 TGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGCAGTTCTTTGTG 
               
               
                 CGGCACGTGTTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGACTGGCTGCACA 
               
               
                 TCGAGTTCACTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCCATGAACATCAT 
               
               
                 CTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCATGTTACACAAG 
               
               
                 CTGAACTCCCAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAACCATGAGACCC 
               
               
                 AGTTTGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCTTCCTGGAGAAG 
               
               
                 AACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAGGAACAAGTTCA 
               
               
                 TCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAGCGCTCGCCCAC 
               
               
                 ACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGGGTGCCTGCCAG 
               
               
                 CCCTTCTTTGTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTGTTCGACCGGCA 
               
               
                 CCTGTGCGTGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAATCCGCCGAGCT 
               
               
                 GGCTACCCCATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTGCTGCTGCCAGG 
               
               
                 TGTGAAGCCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCATGGCTGAGGCT 
               
               
                 GTGCTGGGCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCTGAAGGACCACC 
               
               
                 ATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGTCATCCTCCTTCA 
               
               
                 GAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACA 
               
               
                 CTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGG 
               
               
                 GCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCC 
               
               
                 CGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCG 
               
               
                 CCACCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGC 
               
               
                 TGCACCAACGCCTCAGGGCTGAGGTAAGTATCAAGGTTACAAGACAGGTTAACGGAGACCA 
               
               
                 ATTGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCAGCGCTAGCCCCCGGGTG 
               
               
                 CGCGGCGTCGGTGGTGCCGGCGGGGGGCGCCAGGTCGCAGGCGGTGTAGGGCTCCAGGCA 
               
               
                 GGCGGCGAAGGCCATGACGTGCGCTATGAAGGTCTGCTCCTGCACGCCGTGAACCAGGTGC 
               
               
                 GCCTGCGGGCCGCGCGCGAACACCGCCACGTCCTCGCCTGCGTGGGTCTCTTCGTCCAGGGG 
               
               
                 CACTGCGCACTGCTGCCGATACTCGGGGCTCCCGCTCTCGCTCTCGGTAACATCCGGCCGGG 
               
               
                 CGCCGTCCTTGAGCACATAGCCTGGACCGTTTCGTCGACTGTTAATTAAGCATGCTGGGGAG 
               
               
                 AGATCTGTAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTG 
               
               
                 AGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGA 
               
               
                 GCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 35 is the nucleotide sequence of the CMv2 hybrid back-half vector (i.e., 
               
               
                 AAV-APhead-APSA-ex22hMYO7A-CT.HA-CMv2): 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCAGATCTGGCGCGCCCAATTGGCTTCGAATTCTAGCGGCCGCCCCCGG 
               
               
                 GTGCGCGGCGTCGGTGGTGCCGGCGGGGGGCGCCAGGTCGCAGGCGGTGTAGGGCTCCAG 
               
               
                 GCAGGCGGCGAAGGCCATGACGTGCGCTATGAAGGTCTGCTCCTGCACGCCGTGAACCAGG 
               
               
                 TGCGCCTGCGGGCCGCGCGCGAACACCGCCACGTCCTCGCCTGCGTGGGTCTCTTCGTCCAG 
               
               
                 GGGCACTGCGCACTGCTGCCGATACTCGGGGCTCCCGCTCTCGCTCTCGGTAACATCCGGCC 
               
               
                 GGGCGCCGTCCTTGAGCACATAGCCTGGACCGTTTCTCTTAAGCGACGCATGCTCGCGATA 
               
               
                 GGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGTATCTGTGGCGCCTCG 
               
               
                 AGGCTGAGAAAATGCGGCTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAG 
               
               
                 AAGGCCAAGGAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAG 
               
               
                 GACGCTGAGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAG 
               
               
                 CAGATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGATGTTTG 
               
               
                 GCTTCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGA 
               
               
                 GGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCT 
               
               
                 GCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGTTCGCGGCCACCTAC 
               
               
                 TTCCAGGGGACAACCACGCACTCCTACACCCGGCGGCCACTCAAACAGCCACTGCTCTACC 
               
               
                 ATGACGACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGATCACCATCCTCCGCTTCAT 
               
               
                 GGGGGACCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGATGGCAGTGAGAAGATCCCT 
               
               
                 GTGATGACCAAGATTTATGAGACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCC 
               
               
                 CTGCAGGGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCAC 
               
               
                 AAGCTGGTGCATTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGG 
               
               
                 CTGCATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACCTCC 
               
               
                 AACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAGCACTCCGGGACG 
               
               
                 AGATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAAGAGCAGCTATGCCCG 
               
               
                 GGGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGT 
               
               
                 ACCTGCGGAACTTCATCCACGGGGGCCCGCCCGGCTACGCCCCGTACTGTGAGGAGCGCCT 
               
               
                 GAGAAGGACCTTTGTCAATGGGACACGGACACAGCCGCCCAGCTGGCTGGAGCTGCAGGC 
               
               
                 CACCAAGTCCAAGAAGCCAATCATGTTGCCCGTGACATTCATGGATGGGACCACCAAGACC 
               
               
                 CTGCTGACGGACTCGGCAACCACGGCCAAGGAGCTCTGCAACGCGCTGGCCGACAAGATCT 
               
               
                 CTCTCAAGGACCGGTTCGGGTTCTCCCTCTACATTGCCCTGTTTGACAAGGTGTCCTCCCTG 
               
               
                 GGCAGCGGCAGTGACCACGTCATGGACGCCATCTCCCAGTGCGAGCAGTACGCCAAGGAG 
               
               
                 CAGGGCGCCCAGGAGCGCAACGCCCCCTGGAGGCTCTTCTTCCGCAAAGAGGTCTTCACGC 
               
               
                 CCTGGCACAGCCCCTCCGAGGACAACGTGGCCACCAACCTCATCTACCAGCAGGTGGTGCG 
               
               
                 AGGAGTCAAGTTTGGGGAGTACAGGTGTGAGAAGGAGGACGACCTGGCTGAGCTGGCCTC 
               
               
                 CCAGCAGTACTTTGTAGACTATGGCTCTGAGATGATCCTGGAGCGCCTCCTGAACCTCGTGC 
               
               
                 CCACCTACATCCCCGACCGCGAGATCACGCCCCTGAAGACGCTGGAGAAGTGGGCCCAGCT 
               
               
                 GGCCATCGCCGCCCACAAGAAGGGGATTTATGCCCAGAGGAGAACTGATGCCCAGAAGGT 
               
               
                 CAAAGAGGATGTGGTCAGTTATGCCCGCTTCAAGTGGCCCTTGCTCTTCTCCAGGTTTTATG 
               
               
                 AAGCCTACAAATTCTCAGGCCCCAGTCTCCCCAAGAACGACGTCATCGTGGCCGTCAACTG 
               
               
                 GACGGGTGTGTACTTTGTGGATGAGCAGGAGCAGGTACTTCTGGAGCTGTCCTTCCCAGAG 
               
               
                 ATCATGGCCGTGTCCAGCAGCAGGGGAGCGAAAACGACGGCCCCCAGCTTCACGCTGGCCA 
               
               
                 CCATCAAGGGGGACGAATACACCTTCACCTCCAGCAATGCTGAGGACATTCGTGACCTGGT 
               
               
                 GGTCACCTTCCTAGAGGGGCTCCGGAAGAGATCTAAGTATGTTGTGGCCCTGCAGGATAAC 
               
               
                 CCCAACCCCGCAGGCGAGGAGTCAGGCTTCCTCAGCTTTGCCAAGGGAGACCTCATCATCC 
               
               
                 TGGACCATGACACGGGCGAGCAGGTCATGAACTCGGGCTGGGCCAACGGCATCAATGAGA 
               
               
                 GGACCAAGCAGCGTGGGGACTTCCCCACCGACAGTGTGTACGTCATGCCCACTGTCACCAT 
               
               
                 GCCACCGCGGGAGATTGTGGCCCTGGTCACCATGACTCCCGATCAGAGGCAGGACGTTGTC 
               
               
                 CGGCTCTTGCAGCTGCGAACGGCGGAGCCCGAGGTGCGTGCCAAGCCCTACACGCTGGAGG 
               
               
                 AGTTTTCCTATGACTACTTCAGGCCCCCACCCAAGCACACGCTGAGCCGTGTCATGGTGTCC 
               
               
                 AAGGCCCGAGGCAAGGACCGGCTGTGGAGCCACACGCGGGAACCGCTCAAGCAGGCGCTG 
               
               
                 CTCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGCAGGAGGCCTGCCTGGCCTTCATTGCTG 
               
               
                 TGCTCAAGTACATGGGCGACTACCCGTCCAAGAGGACACGCTCCGTCAACGAGCTCACCGA 
               
               
                 CCAGATCTTTGAGGGTCCCCTGAAAGCCGAGCCCCTGAAGGACGAGGCATATGTGCAGATC 
               
               
                 CTGAAGCAGCTGACCGACAACCACATCAGGTACAGCGAGGAGCGGGGTTGGGAGCTGCTC 
               
               
                 TGGCTGTGCACGGGCCTTTTCCCACCCAGCAACATCCTCCTGCCCCACGTGCAGCGCTTCCT 
               
               
                 GCAGTCCCGAAAGCACTGCCCACTCGCCATCGACTGCCTGCAACGGCTCCAGAAAGCCCTG 
               
               
                 AGAAACGGGTCCCGGAAGTACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAGCACAAG 
               
               
                 ACCACCCAGATTTTCCACAAAGTCTACTTCCCTGATGACACTGACGAGGCCTTCGAAGTGG 
               
               
                 AGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCACCAGGCTGCTCCTCAAGTC 
               
               
                 CTCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGACAAGGTCATCAGCGTTCCTGAGAAT 
               
               
                 GACTTCTTCTTTGACTTTGTTCGACACTTGACAGACTGGATAAAGAAAGCTCGGCCCATCAA 
               
               
                 GGACGGAATTGTGCCCTCACTCACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGACCACC 
               
               
                 ACGGTGCCAGGGAAGGATCCCATGGCCGATTCCATCTTCCACTATTACCAGGAGTTGCCCA 
               
               
                 AGTATCTCCGAGGCTACCACAAGTGCACGCGGGAGGAGGTGCTGCAGCTGGGGGCGCTGAT 
               
               
                 CTACAGGGTCAAGTTCGAGGAGGACAAGTCCTACTTCCCCAGCATCCCCAAGCTGCTGCGG 
               
               
                 GAGCTGGTGCCCCAGGACCTTATCCGGCAGGTCTCACCTGATGACTGGAAGCGGTCCATCG 
               
               
                 TCGCCTACTTCAACAAGCACGCAGGGAAGTCCAAGGAGGAGGCCAAGCTGGCCTTCCTGAA 
               
               
                 GCTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTCGAGGTGAAGCAAACTACGGAGC 
               
               
                 CAAACTTCCCTGAGATCCTCCTAATTGCCATCAACAAGTATGGGGTCAGCCTCATCGATCCC 
               
               
                 AAAACGAAGGATATCCTCACCACTCATCCCTTCACCAAGATCTCCAACTGGAGCAGCGGCA 
               
               
                 ACACCTACTTCCACATCACCATTGGGAACTTGGTGCGCGGGAGCAAACTGCTCTGCGAGAC 
               
               
                 GTCACTGGGCTACAAGATGGATGACCTCCTGACTTCCTACATTAGCCAGATGCTCACAGCC 
               
               
                 ATGAGCAAACAGCGGGGCTCCAGGAGCGGCAAGTACCCTTACGATGTACCGGATTACGCAT 
               
               
                 GAGGTACCAAGGGCGAATTCTGCAGTCGACTAGAGCTCGCTGATCAGCCTCGACTGTGCCT 
               
               
                 TCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCC 
               
               
                 ACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCA 
               
               
                 TTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAG 
               
               
                 CAGGCATGCTGGGGAGAGATCTGGAGGACTAGTCCGTCGACTGTTAATTAAGCATGCTGGG 
               
               
                 GAGAGATCTAGGAAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCT 
               
               
                 CACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTG 
               
               
                 AGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 36 is the nucleotide sequence of the CMvl overlap front-half vector (i.e., 
               
               
                 AAV-smCBA-hMYO7A-noDimNT-CMvl): 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCAATTCGGTACCCTAGTTATTAATAGTAATCA 
               
               
                 ATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA 
               
               
                 TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC 
               
               
                 CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAAC 
               
               
                 TGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG 
               
               
                 ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGG 
               
               
                 CAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTT 
               
               
                 CACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTT 
               
               
                 TGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGG 
               
               
                 CGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGC 
               
               
                 TCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGC 
               
               
                 GCGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCG 
               
               
                 CGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCC 
               
               
                 CTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC 
               
               
                 GTGAAAGCCTTGAGGGGCTCCGGGAGCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCT 
               
               
                 TTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAA 
               
               
                 TTCTAGCGGCCGCCACCATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAG 
               
               
                 ATTGGGGCAGGAGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAG 
               
               
                 GTCCAGGTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACA 
               
               
                 TCAAGCCTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCT 
               
               
                 CAACGAGGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACG 
               
               
                 TATACGGGCTCCATCCTGGTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGA 
               
               
                 GCACATCCGCCAGTATACCAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATT 
               
               
                 GCTGACAACTGCTACTTCAACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTG 
               
               
                 GGGAATCTGGGGCCGGGAAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCA 
               
               
                 TCAGTGGGCAGCACTCGTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGC 
               
               
                 ATTTGGGAATGCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGAC 
               
               
                 ATCCACTTCAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAA 
               
               
                 AAGTCACGTGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGC 
               
               
                 TGGAGGGTATGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACA 
               
               
                 ACTACTTGGCCATGGGTAACTGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGC 
               
               
                 CAACATCCGCTCCGCCATGAAGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCG 
               
               
                 AAGCTCCTGGCTGCCATCCTGCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAA 
               
               
                 ACCTGGATGCCTGTGAGGTTCTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAG 
               
               
                 GTGAACCCCCCAGACCTGATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGA 
               
               
                 CGGTGTCCACCCCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGG 
               
               
                 GATCTACGGGCGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCT 
               
               
                 CCCTCCCAGGATGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGA 
               
               
                 GAACTTTGCTGTGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGC 
               
               
                 AGTTCTTTGTGCGGCACGTGTTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGA 
               
               
                 CTGGCTGCACATCGAGTTCACTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCC 
               
               
                 ATGAACATCATCTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCA 
               
               
                 TGTTACACAAGCTGAACTCCCAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAA 
               
               
                 CCATGAGACCCAGTTTGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCT 
               
               
                 TCCTGGAGAAGAACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAG 
               
               
                 GAACAAGTTCATCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAG 
               
               
                 CGCTCGCCCACACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGG 
               
               
                 GTGCCTGCCAGCCCTTCTTTGTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTG 
               
               
                 TTCGACCGGCACCTGTGCGTGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAA 
               
               
                 TCCGCCGAGCTGGCTACCCCATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTG 
               
               
                 CTGCTGCCAGGTGTGAAGCCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCA 
               
               
                 TGGCTGAGGCTGTGCTGGGCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCT 
               
               
                 GAAGGACCACCATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGT 
               
               
                 CATCCTCCTTCAGAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAG 
               
               
                 AACGCTGCCACACTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGG 
               
               
                 CTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGC 
               
               
                 AGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTG 
               
               
                 CGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCA 
               
               
                 TGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGA 
               
               
                 GAAAATGCGGCTGGCGGAGGAAGAGAAGCTTAGAGGATCCTCCCGTCGACTGTTTAAGCAT 
               
               
                 GCTGGGGAGAGATCTGAGGAAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCT 
               
               
                 CGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGG 
               
               
                 CCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 62 (peptide encoded by the N-myosin7A portion of SEQ ID NO: 36 
               
               
                 (AAV-smCBA-hMYO7A-noDimNT-CMv1)) 
               
               
                 MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPT 
               
               
                 SVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIG 
               
               
                 EMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATP 
               
               
                 ILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCML 
               
               
                 EGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKL 
               
               
                 LAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLS 
               
               
                 REQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQL 
               
               
                 CINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPK 
               
               
                 GTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVH 
               
               
                 SSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLF 
               
               
                 DRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAE 
               
               
                 AVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLI 
               
               
                 QRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHR 
               
               
                 LWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKL 
               
               
                   
               
               
                 SEQ ID NO: 63 (only the N-myosin7A portion of SEQ ID NO: 36 (AAV-smCBA- 
               
               
                 hMYO7A-noDimNT-CMv1)) 
               
               
                 ATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAGATTGGGGCAGGAGTTCG 
               
               
                 ACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAGGTCCAGGTGGTGGATGA 
               
               
                 TGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACATCAAGCCTATGCACCCC 
               
               
                 ACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCTCAACGAGGCGGGCATCT 
               
               
                 TGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACGTATACGGGCTCCATCCTG 
               
               
                 GTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGAGCACATCCGCCAGTATAC 
               
               
                 CAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATTGCTGACAACTGCTACTTC 
               
               
                 AACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTGGGGAATCTGGGGCCGGG 
               
               
                 AAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCATCAGTGGGCAGCACTCGT 
               
               
                 GGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGCATTTGGGAATGCCAAGAC 
               
               
                 CATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGACATCCACTTCAACAAGCGG 
               
               
                 GGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAAAAGTCACGTGTCTGTCGCC 
               
               
                 AGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGCTGGAGGGTATGAGTGAGGA 
               
               
                 TCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACAACTACTTGGCCATGGGTAAC 
               
               
                 TGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGCCAACATCCGCTCCGCCATGA 
               
               
                 AGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCGAAGCTCCTGGCTGCCATCCT 
               
               
                 GCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAAACCTGGATGCCTGTGAGGTT 
               
               
                 CTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAGGTGAACCCCCCAGACCTGAT 
               
               
                 GAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGACGGTGTCCACCCCACTGAGC 
               
               
                 AGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGGGATCTACGGGCGGCTGTTCG 
               
               
                 TGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCTCCCTCCCAGGATGTGAAGAA 
               
               
                 CTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGAGAACTTTGCTGTGAACAGCT 
               
               
                 TTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGCAGTTCTTTGTGCGGCACGTG 
               
               
                 TTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGACTGGCTGCACATCGAGTTCA 
               
               
                 CTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCCATGAACATCATCTCCCTCAT 
               
               
                 CGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCATGTTACACAAGCTGAACTCC 
               
               
                 CAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAACCATGAGACCCAGTTTGGCA 
               
               
                 TCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCTTCCTGGAGAAGAACCGAGA 
               
               
                 CACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAGGAACAAGTTCATCAAGCAG 
               
               
                 ATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAGCGCTCGCCCACACTTAGCA 
               
               
                 GCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGGGTGCCTGCCAGCCCTTCTTT 
               
               
                 GTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTGTTCGACCGGCACCTGTGCG 
               
               
                 TGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAATCCGCCGAGCTGGCTACCC 
               
               
                 CATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTGCTGCTGCCAGGTGTGAAG 
               
               
                 CCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCATGGCTGAGGCTGTGCTGG 
               
               
                 GCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCTGAAGGACCACCATGACAT 
               
               
                 GCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGTCATCCTCCTTCAGAAAGTC 
               
               
                 ATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCC 
               
               
                 AGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCT 
               
               
                 GCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAG 
               
               
                 CGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCG 
               
               
                 CCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCAC 
               
               
                 CAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGG 
               
               
                 AAGAGAAGCTT 
               
               
                   
               
               
                 SEQ ID NO: 37 is the nucleotide sequence of the second generation overlap front-half 
               
               
                 vector (i.e., AAV-smCBA-hMYO7A-noDIM-NTlong): 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCAATTCGGTACCCTAGTTATTAATAGTAATCA 
               
               
                 ATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA 
               
               
                 TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC 
               
               
                 CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAAC 
               
               
                 TGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG 
               
               
                 ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGG 
               
               
                 CAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTT 
               
               
                 CACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTT 
               
               
                 TGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGG 
               
               
                 CGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGC 
               
               
                 TCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGC 
               
               
                 GCGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCG 
               
               
                 CGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCC 
               
               
                 CTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC 
               
               
                 GTGAAAGCCTTGAGGGGCTCCGGGAGCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCT 
               
               
                 TTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAA 
               
               
                 TTCTAGCGGCCGCCACCATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAG 
               
               
                 ATTGGGGCAGGAGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAG 
               
               
                 GTCCAGGTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACA 
               
               
                 TCAAGCCTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCT 
               
               
                 CAACGAGGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACG 
               
               
                 TATACGGGCTCCATCCTGGTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGA 
               
               
                 GCACATCCGCCAGTATACCAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATT 
               
               
                 GCTGACAACTGCTACTTCAACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTG 
               
               
                 GGGAATCTGGGGCCGGGAAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCA 
               
               
                 TCAGTGGGCAGCACTCGTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGC 
               
               
                 ATTTGGGAATGCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGAC 
               
               
                 ATCCACTTCAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAA 
               
               
                 AAGTCACGTGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGC 
               
               
                 TGGAGGGTATGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACA 
               
               
                 ACTACTTGGCCATGGGTAACTGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGC 
               
               
                 CAACATCCGCTCCGCCATGAAGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCG 
               
               
                 AAGCTCCTGGCTGCCATCCTGCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAA 
               
               
                 ACCTGGATGCCTGTGAGGTTCTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAG 
               
               
                 GTGAACCCCCCAGACCTGATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGA 
               
               
                 CGGTGTCCACCCCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGG 
               
               
                 GATCTACGGGCGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCT 
               
               
                 CCCTCCCAGGATGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGA 
               
               
                 GAACTTTGCTGTGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGC 
               
               
                 AGTTCTTTGTGCGGCACGTGTTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGA 
               
               
                 CTGGCTGCACATCGAGTTCACTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCC 
               
               
                 ATGAACATCATCTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCA 
               
               
                 TGTTACACAAGCTGAACTCCCAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAA 
               
               
                 CCATGAGACCCAGTTTGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCT 
               
               
                 TCCTGGAGAAGAACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAG 
               
               
                 GAACAAGTTCATCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAG 
               
               
                 CGCTCGCCCACACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGG 
               
               
                 GTGCCTGCCAGCCCTTCTTTGTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTG 
               
               
                 TTCGACCGGCACCTGTGCGTGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAA 
               
               
                 TCCGCCGAGCTGGCTACCCCATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTG 
               
               
                 CTGCTGCCAGGTGTGAAGCCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCA 
               
               
                 TGGCTGAGGCTGTGCTGGGCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCT 
               
               
                 GAAGGACCACCATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGT 
               
               
                 CATCCTCCTTCAGAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAG 
               
               
                 AACGCTGCCACACTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGG 
               
               
                 CTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGC 
               
               
                 AGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTG 
               
               
                 CGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCA 
               
               
                 TGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGA 
               
               
                 GAAAATGCGGCTGGCGGAGGAAGAGAAGCTTTGAAAGTGACATTAGGCTCCCGTCGACTGT 
               
               
                 TAATTAAGCATGCTGGGGAGAGATCTGAGGAAACCCCTAGTGATGGAGTTGGCCACTCCCT 
               
               
                 CTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTT 
               
               
                 TGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 90 (only the N-myosin7A portion of SEQ ID NO: 37 (AAV-smCBA-hMYO7A- 
               
               
                 noDIM-NTlong)) 
               
               
                 ATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAGATTGGGGCAGGAGTTCG 
               
               
                 ACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAGGTCCAGGTGGTGGATGA 
               
               
                 TGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACATCAAGCCTATGCACCCC 
               
               
                 ACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCTCAACGAGGCGGGCATCT 
               
               
                 TGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACGTATACGGGCTCCATCCTG 
               
               
                 GTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGAGCACATCCGCCAGTATAC 
               
               
                 CAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATTGCTGACAACTGCTACTTC 
               
               
                 AACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTGGGGAATCTGGGGCCGGG 
               
               
                 AAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCATCAGTGGGCAGCACTCGT 
               
               
                 GGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGCATTTGGGAATGCCAAGAC 
               
               
                 CATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGACATCCACTTCAACAAGCGG 
               
               
                 GGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAAAAGTCACGTGTCTGTCGCC 
               
               
                 AGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGCTGGAGGGTATGAGTGAGGA 
               
               
                 TCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACAACTACTTGGCCATGGGTAAC 
               
               
                 TGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGCCAACATCCGCTCCGCCATGA 
               
               
                 AGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCGAAGCTCCTGGCTGCCATCCT 
               
               
                 GCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAAACCTGGATGCCTGTGAGGTT 
               
               
                 CTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAGGTGAACCCCCCAGACCTGAT 
               
               
                 GAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGACGGTGTCCACCCCACTGAGC 
               
               
                 AGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGGGATCTACGGGCGGCTGTTCG 
               
               
                 TGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCTCCCTCCCAGGATGTGAAGAA 
               
               
                 CTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGAGAACTTTGCTGTGAACAGCT 
               
               
                 TTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGCAGTTCTTTGTGCGGCACGTG 
               
               
                 TTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGACTGGCTGCACATCGAGTTCA 
               
               
                 CTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCCATGAACATCATCTCCCTCAT 
               
               
                 CGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCATGTTACACAAGCTGAACTCC 
               
               
                 CAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAACCATGAGACCCAGTTTGGCA 
               
               
                 TCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCTTCCTGGAGAAGAACCGAGA 
               
               
                 CACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAGGAACAAGTTCATCAAGCAG 
               
               
                 ATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAGCGCTCGCCCACACTTAGCA 
               
               
                 GCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGGGTGCCTGCCAGCCCTTCTTT 
               
               
                 GTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTGTTCGACCGGCACCTGTGCG 
               
               
                 TGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAATCCGCCGAGCTGGCTACCC 
               
               
                 CATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTGCTGCTGCCAGGTGTGAAG 
               
               
                 CCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCATGGCTGAGGCTGTGCTGG 
               
               
                 GCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCTGAAGGACCACCATGACAT 
               
               
                 GCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGTCATCCTCCTTCAGAAAGTC 
               
               
                 ATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCC 
               
               
                 AGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCT 
               
               
                 GCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAG 
               
               
                 CGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCG 
               
               
                 CCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCAC 
               
               
                 CAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGG 
               
               
                 AAGAGAAGCTT 
               
               
                   
               
               
                 SEQ ID NO: 91 (only the N-myosin7A portion of SEQ ID NO: 37 (AAV-smCBA-hMYO7A- 
               
               
                 noDIM-NTlong)) 
               
               
                 MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPT 
               
               
                 SVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIG 
               
               
                 EMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATP 
               
               
                 ILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCML 
               
               
                 EGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKL 
               
               
                 LAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLS 
               
               
                 REQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQL 
               
               
                 CINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPK 
               
               
                 GTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVH 
               
               
                 SSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLF 
               
               
                 DRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAE 
               
               
                 AVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLI 
               
               
                 QRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHR 
               
               
                 LWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKL 
               
               
                   
               
               
                 SEQ ID NO: 38 is the nucleotide sequence of the second generation overlap back-half 
               
               
                 vector (i.e., AAV-hMYO7A-CTlong-v2.HA). 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCAGATCTGGCGCGCCGGATCCGGGCTGATGCGTCTGGGCTTCCTGCGG 
               
               
                 CTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAGCGCA 
               
               
                 TCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTC 
               
               
                 TGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCACCAAC 
               
               
                 GCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGGAAG 
               
               
                 AGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAAGCAT 
               
               
                 CAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAAGGAG 
               
               
                 GCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGCCTGTC 
               
               
                 AATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGCCAG 
               
               
                 GCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGATGG 
               
               
                 TGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGA 
               
               
                 GTATAAATTTGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACC 
               
               
                 CGGCGGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCCC 
               
               
                 TGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCACAC 
               
               
                 AGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTGGGC 
               
               
                 AAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTCCCC 
               
               
                 GAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTGCATTTGACTCTGAAAAAGAAG 
               
               
                 TCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGCATGACGGGGAGTCCACAGTGCAGGGC 
               
               
                 AACAGCATGCTGGAGGACCGGCCCACCTCCAACCTGGAGAAGCTGCACTTCATCATCGGCA 
               
               
                 ATGGCATCCTGCGGCCAGCACTCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCTGAC 
               
               
                 CCACAACCCCTCCAAGAGCAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGGCT 
               
               
                 GTTTCGCCCCCTCCGAGAAGTTTGTCAAGTACCTGCGGAACTTCATCCACGGGGGCCCGCCC 
               
               
                 GGCTACGCCCCGTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTCAATGGGACACGGACAC 
               
               
                 AGCCGCCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCAATCATGTTGCCCGT 
               
               
                 GACATTCATGGATGGGACCACCAAGACCCTGCTGACGGACTCGGCAACCACGGCCAAGGA 
               
               
                 GCTCTGCAACGCGCTGGCCGACAAGATCTCTCTCAAGGACCGGTTCGGGTTCTCCCTCTACA 
               
               
                 TTGCCCTGTTTGACAAGGTGTCCTCCCTGGGCAGCGGCAGTGACCACGTCATGGACGCCATC 
               
               
                 TCCCAGTGCGAGCAGTACGCCAAGGAGCAGGGCGCCCAGGAGCGCAACGCCCCCTGGAGG 
               
               
                 CTCTTCTTCCGCAAAGAGGTCTTCACGCCCTGGCACAGCCCCTCCGAGGACAACGTGGCCA 
               
               
                 CCAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGAGAA 
               
               
                 GGAGGACGACCTGGCTGAGCTGGCCTCCCAGCAGTACTTTGTAGACTATGGCTCTGAGATG 
               
               
                 ATCCTGGAGCGCCTCCTGAACCTCGTGCCCACCTACATCCCCGACCGCGAGATCACGCCCCT 
               
               
                 GAAGACGCTGGAGAAGTGGGCCCAGCTGGCCATCGCCGCCCACAAGAAGGGGATTTATGC 
               
               
                 CCAGAGGAGAACTGATGCCCAGAAGGTCAAAGAGGATGTGGTCAGTTATGCCCGCTTCAAG 
               
               
                 TGGCCCTTGCTCTTCTCCAGGTTTTATGAAGCCTACAAATTCTCAGGCCCCAGTCTCCCCAA 
               
               
                 GAACGACGTCATCGTGGCCGTCAACTGGACGGGTGTGTACTTTGTGGATGAGCAGGAGCAG 
               
               
                 GTACTTCTGGAGCTGTCCTTCCCAGAGATCATGGCCGTGTCCAGCAGCAGGGGAGCGAAAA 
               
               
                 CGACGGCCCCCAGCTTCACGCTGGCCACCATCAAGGGGGACGAATACACCTTCACCTCCAG 
               
               
                 CAATGCTGAGGACATTCGTGACCTGGTGGTCACCTTCCTAGAGGGGCTCCGGAAGAGATCT 
               
               
                 AAGTATGTTGTGGCCCTGCAGGATAACCCCAACCCCGCAGGCGAGGAGTCAGGCTTCCTCA 
               
               
                 GCTTTGCCAAGGGAGACCTCATCATCCTGGACCATGACACGGGCGAGCAGGTCATGAACTC 
               
               
                 GGGCTGGGCCAACGGCATCAATGAGAGGACCAAGCAGCGTGGGGACTTCCCCACCGACAG 
               
               
                 TGTGTACGTCATGCCCACTGTCACCATGCCACCGCGGGAGATTGTGGCCCTGGTCACCATGA 
               
               
                 CTCCCGATCAGAGGCAGGACGTTGTCCGGCTCTTGCAGCTGCGAACGGCGGAGCCCGAGGT 
               
               
                 GCGTGCCAAGCCCTACACGCTGGAGGAGTTTTCCTATGACTACTTCAGGCCCCCACCCAAG 
               
               
                 CACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGAGGCAAGGACCGGCTGTGGAGCCACA 
               
               
                 CGCGGGAACCGCTCAAGCAGGCGCTGCTCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGCA 
               
               
                 GGAGGCCTGCCTGGCCTTCATTGCTGTGCTCAAGTACATGGGCGACTACCCGTCCAAGAGG 
               
               
                 ACACGCTCCGTCAACGAGCTCACCGACCAGATCTTTGAGGGTCCCCTGAAAGCCGAGCCCC 
               
               
                 TGAAGGACGAGGCATATGTGCAGATCCTGAAGCAGCTGACCGACAACCACATCAGGTACA 
               
               
                 GCGAGGAGCGGGGTTGGGAGCTGCTCTGGCTGTGCACGGGCCTTTTCCCACCCAGCAACAT 
               
               
                 CCTCCTGCCCCACGTGCAGCGCTTCCTGCAGTCCCGAAAGCACTGCCCACTCGCCATCGACT 
               
               
                 GCCTGCAACGGCTCCAGAAAGCCCTGAGAAACGGGTCCCGGAAGTACCCTCCGCACCTGGT 
               
               
                 GGAGGTGGAGGCCATCCAGCACAAGACCACCCAGATTTTCCACAAAGTCTACTTCCCTGAT 
               
               
                 GACACTGACGAGGCCTTCGAAGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAAC 
               
               
                 ATCGCCACCAGGCTGCTCCTCAAGTCCTCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGA 
               
               
                 CAAGGTCATCAGCGTTCCTGAGAATGACTTCTTCTTTGACTTTGTTCGACACTTGACAGACT 
               
               
                 GGATAAAGAAAGCTCGGCCCATCAAGGACGGAATTGTGCCCTCACTCACCTACCAGGTGTT 
               
               
                 CTTCATGAAGAAGCTGTGGACCACCACGGTGCCAGGGAAGGATCCCATGGCCGATTCCATC 
               
               
                 TTCCACTATTACCAGGAGTTGCCCAAGTATCTCCGAGGCTACCACAAGTGCACGCGGGAGG 
               
               
                 AGGTGCTGCAGCTGGGGGCGCTGATCTACAGGGTCAAGTTCGAGGAGGACAAGTCCTACTT 
               
               
                 CCCCAGCATCCCCAAGCTGCTGCGGGAGCTGGTGCCCCAGGACCTTATCCGGCAGGTCTCA 
               
               
                 CCTGATGACTGGAAGCGGTCCATCGTCGCCTACTTCAACAAGCACGCAGGGAAGTCCAAGG 
               
               
                 AGGAGGCCAAGCTGGCCTTCCTGAAGCTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTC 
               
               
                 TTCGAGGTGAAGCAAACTACGGAGCCAAACTTCCCTGAGATCCTCCTAATTGCCATCAACA 
               
               
                 AGTATGGGGTCAGCCTCATCGATCCCAAAACGAAGGATATCCTCACCACTCATCCCTTCACC 
               
               
                 AAGATCTCCAACTGGAGCAGCGGCAACACCTACTTCCACATCACCATTGGGAACTTGGTGC 
               
               
                 GCGGGAGCAAACTGCTCTGCGAGACGTCACTGGGCTACAAGATGGATGACCTCCTGACTTC 
               
               
                 CTACATTAGCCAGATGCTCACAGCCATGAGCAAACAGCGGGGCTCCAGGAGCGGCAAGTA 
               
               
                 CCCTTACGATGTACCGGATTACGCATGAGGTACCAAGGGCGAATTCTGCAGTCGACTAGAG 
               
               
                 CTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCG 
               
               
                 TGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATT 
               
               
                 GCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCA 
               
               
                 AGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGAGAGATCTGAGGATCCTTAAT 
               
               
                 TAAGCATGCTGGGGAGAGATCTGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGC 
               
               
                 GCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGG 
               
               
                 CGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 77 (C-term myosin7A (e.g., AAV-hMYO7A-CTlong-v2.HA)) 
               
               
                 GGGCTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACC 
               
               
                 AGCAGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCT 
               
               
                 GGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGG 
               
               
                 GGCATGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGG 
               
               
                 CTGAGAAAATGCGGCTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAG 
               
               
                 GCCAAGGAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGAC 
               
               
                 GCTGAGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAG 
               
               
                 ATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCT 
               
               
                 TCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGA 
               
               
                 CCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCC 
               
               
                 TGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTC 
               
               
                 CAGGGGACAACCACGCACTCCTACACCCGGCGGCCACTCAAACAGCCACTGCTCTACCATG 
               
               
                 ACGACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGATCACCATCCTCCGCTTCATGGG 
               
               
                 GGACCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTG 
               
               
                 ATGACCAAGATTTATGAGACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTG 
               
               
                 CAGGGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAG 
               
               
                 CTGGTGCATTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGC 
               
               
                 ATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACCTCCAACC 
               
               
                 TGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAGCACTCCGGGACGAGAT 
               
               
                 CTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAAGAGCAGCTATGCCCGGGGC 
               
               
                 TGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGTACCT 
               
               
                 GCGGAACTTCATCCACGGGGGCCCGCCCGGCTACGCCCCGTACTGTGAGGAGCGCCTGAGA 
               
               
                 AGGACCTTTGTCAATGGGACACGGACACAGCCGCCCAGCTGGCTGGAGCTGCAGGCCACCA 
               
               
                 AGTCCAAGAAGCCAATCATGTTGCCCGTGACATTCATGGATGGGACCACCAAGACCCTGCT 
               
               
                 GACGGACTCGGCAACCACGGCCAAGGAGCTCTGCAACGCGCTGGCCGACAAGATCTCTCTC 
               
               
                 AAGGACCGGTTCGGGTTCTCCCTCTACATTGCCCTGTTTGACAAGGTGTCCTCCCTGGGCAG 
               
               
                 CGGCAGTGACCACGTCATGGACGCCATCTCCCAGTGCGAGCAGTACGCCAAGGAGCAGGG 
               
               
                 CGCCCAGGAGCGCAACGCCCCCTGGAGGCTCTTCTTCCGCAAAGAGGTCTTCACGCCCTGG 
               
               
                 CACAGCCCCTCCGAGGACAACGTGGCCACCAACCTCATCTACCAGCAGGTGGTGCGAGGAG 
               
               
                 TCAAGTTTGGGGAGTACAGGTGTGAGAAGGAGGACGACCTGGCTGAGCTGGCCTCCCAGCA 
               
               
                 GTACTTTGTAGACTATGGCTCTGAGATGATCCTGGAGCGCCTCCTGAACCTCGTGCCCACCT 
               
               
                 ACATCCCCGACCGCGAGATCACGCCCCTGAAGACGCTGGAGAAGTGGGCCCAGCTGGCCAT 
               
               
                 CGCCGCCCACAAGAAGGGGATTTATGCCCAGAGGAGAACTGATGCCCAGAAGGTCAAAGA 
               
               
                 GGATGTGGTCAGTTATGCCCGCTTCAAGTGGCCCTTGCTCTTCTCCAGGTTTTATGAAGCCT 
               
               
                 ACAAATTCTCAGGCCCCAGTCTCCCCAAGAACGACGTCATCGTGGCCGTCAACTGGACGGG 
               
               
                 TGTGTACTTTGTGGATGAGCAGGAGCAGGTACTTCTGGAGCTGTCCTTCCCAGAGATCATGG 
               
               
                 CCGTGTCCAGCAGCAGGGGAGCGAAAACGACGGCCCCCAGCTTCACGCTGGCCACCATCAA 
               
               
                 GGGGGACGAATACACCTTCACCTCCAGCAATGCTGAGGACATTCGTGACCTGGTGGTCACC 
               
               
                 TTCCTAGAGGGGCTCCGGAAGAGATCTAAGTATGTTGTGGCCCTGCAGGATAACCCCAACC 
               
               
                 CCGCAGGCGAGGAGTCAGGCTTCCTCAGCTTTGCCAAGGGAGACCTCATCATCCTGGACCA 
               
               
                 TGACACGGGCGAGCAGGTCATGAACTCGGGCTGGGCCAACGGCATCAATGAGAGGACCAA 
               
               
                 GCAGCGTGGGGACTTCCCCACCGACAGTGTGTACGTCATGCCCACTGTCACCATGCCACCG 
               
               
                 CGGGAGATTGTGGCCCTGGTCACCATGACTCCCGATCAGAGGCAGGACGTTGTCCGGCTCT 
               
               
                 TGCAGCTGCGAACGGCGGAGCCCGAGGTGCGTGCCAAGCCCTACACGCTGGAGGAGTTTTC 
               
               
                 CTATGACTACTTCAGGCCCCCACCCAAGCACACGCTGAGCCGTGTCATGGTGTCCAAGGCC 
               
               
                 CGAGGCAAGGACCGGCTGTGGAGCCACACGCGGGAACCGCTCAAGCAGGCGCTGCTCAAG 
               
               
                 AAGCTCCTGGGCAGTGAGGAGCTCTCGCAGGAGGCCTGCCTGGCCTTCATTGCTGTGCTCA 
               
               
                 AGTACATGGGCGACTACCCGTCCAAGAGGACACGCTCCGTCAACGAGCTCACCGACCAGAT 
               
               
                 CTTTGAGGGTCCCCTGAAAGCCGAGCCCCTGAAGGACGAGGCATATGTGCAGATCCTGAAG 
               
               
                 CAGCTGACCGACAACCACATCAGGTACAGCGAGGAGCGGGGTTGGGAGCTGCTCTGGCTGT 
               
               
                 GCACGGGCCTTTTCCCACCCAGCAACATCCTCCTGCCCCACGTGCAGCGCTTCCTGCAGTCC 
               
               
                 CGAAAGCACTGCCCACTCGCCATCGACTGCCTGCAACGGCTCCAGAAAGCCCTGAGAAACG 
               
               
                 GGTCCCGGAAGTACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAGCACAAGACCACCCA 
               
               
                 GATTTTCCACAAAGTCTACTTCCCTGATGACACTGACGAGGCCTTCGAAGTGGAGTCCAGC 
               
               
                 ACCAAGGCCAAGGACTTCTGCCAGAACATCGCCACCAGGCTGCTCCTCAAGTCCTCAGAGG 
               
               
                 GATTCAGCCTCTTTGTCAAAATTGCAGACAAGGTCATCAGCGTTCCTGAGAATGACTTCTTC 
               
               
                 TTTGACTTTGTTCGACACTTGACAGACTGGATAAAGAAAGCTCGGCCCATCAAGGACGGAA 
               
               
                 TTGTGCCCTCACTCACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGACCACCACGGTGCCA 
               
               
                 GGGAAGGATCCCATGGCCGATTCCATCTTCCACTATTACCAGGAGTTGCCCAAGTATCTCCG 
               
               
                 AGGCTACCACAAGTGCACGCGGGAGGAGGTGCTGCAGCTGGGGGCGCTGATCTACAGGGT 
               
               
                 CAAGTTCGAGGAGGACAAGTCCTACTTCCCCAGCATCCCCAAGCTGCTGCGGGAGCTGGTG 
               
               
                 CCCCAGGACCTTATCCGGCAGGTCTCACCTGATGACTGGAAGCGGTCCATCGTCGCCTACTT 
               
               
                 CAACAAGCACGCAGGGAAGTCCAAGGAGGAGGCCAAGCTGGCCTTCCTGAAGCTCATCTTC 
               
               
                 AAGTGGCCCACCTTTGGCTCAGCCTTCTTCGAGGTGAAGCAAACTACGGAGCCAAACTTCC 
               
               
                 CTGAGATCCTCCTAATTGCCATCAACAAGTATGGGGTCAGCCTCATCGATCCCAAAACGAA 
               
               
                 GGATATCCTCACCACTCATCCCTTCACCAAGATCTCCAACTGGAGCAGCGGCAACACCTACT 
               
               
                 TCCACATCACCATTGGGAACTTGGTGCGCGGGAGCAAACTGCTCTGCGAGACGTCACTGGG 
               
               
                 CTACAAGATGGATGACCTCCTGACTTCCTACATTAGCCAGATGCTCACAGCCATGAGCAAA 
               
               
                 CAGCGGGGCTCCAGGAGCGGCAAG 
               
               
                   
               
               
                 SEQ ID NO: 78 (N-term myosin7A (e.g., AAV-hMYO7A-CTlong.HA) 
               
               
                 GLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARG 
               
               
                 MIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAER 
               
               
                 ELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERG 
               
               
                 RREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQL 
               
               
                 AALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPE 
               
               
                 GQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRP 
               
               
                 ALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEER 
               
               
                 LRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLK 
               
               
                 DRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPS 
               
               
                 EDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREI 
               
               
                 TPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLP 
               
               
                 KNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAE 
               
               
                 DIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGI 
               
               
                 NERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLE 
               
               
                 EFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLK 
               
               
                 YMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGL 
               
               
                 FPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFP 
               
               
                 DDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKK 
               
               
                 ARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLG 
               
               
                 ALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLI 
               
               
                 FKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIG 
               
               
                 NLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK 
               
            
           
         
       
     
     In some embodiments, the vectors provided herein comprise a truncated chimeric CBA promoter. The vectors provided herein may comprise a promoter having a nucleotide sequence that differs from the smCBA promoter set forth as SEQ ID NO: 64 by 1, 2, or 3 nucleotides. In some embodiments, the vectors provided herein comprise a promoter that is not an smCBA promoter. In some embodiments, the vectors provided herein comprise a promoter selected from selected from the group consisting of a CMV promoter, an EF-1 alpha promoter, a cone arrestin promoter, a human myosin 7a gene-derived promoter, a TαC gene-derived promoter, a rhodopsin promoter, a cGMP-phosphodiesterase β-subunit promoter, human or mouse rhodopsin promoter, a hGRK1 promoter, a synapsin promoter, a glial fibrillary acidic protein (GFAP) promoter, a rod specific IRBP promoter, a VMD2 promoter, and combinations thereof. In some embodiments, the promoter is a rhodopsin promoter. In some embodiments, the promoter is a CMV promoter. In some embodiments, the promoter is not a CMV promoter. 
     In some embodiments, the promoter is a tissue-specific promoter. In some embodiments, the promoter mediates expression in ocular tissue. In some embodiments, the promoter does not mediate expression in ocular tissue. In some embodiments, the promoter mediates expression in hair cells of the auditory system and/or the vestibular system. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 64 smCBA promoter 
                   
               
               
                 AATTCGGTACCCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA 
               
               
                 TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCC 
               
               
                 CCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATT 
               
               
                 GACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCA 
               
               
                 TATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCC 
               
               
                 AGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATT 
               
               
                 ACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACC 
               
               
                 CCCAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCGGGGGGGGGGG 
               
               
                 GGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGA 
               
               
                 GGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGC 
               
               
                 GGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGCGCGCTGCC 
               
               
                 TTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG 
               
               
                 TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGT 
               
               
                 TTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAGCTAG 
               
               
                 AGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGT 
               
               
                 TATTGTGCTGTCTCATCATTTTGGCAAAG 
               
               
                   
               
               
                 SEQ ID NO: 39 is the nucleotide sequence of second generation overlapping portion of the 
               
               
                 overlap vector system: 
               
               
                 CAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCCAGAGGCACTGGC 
               
               
                 GGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCTGCGGCTG 
               
               
                 CAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAGCG 
               
               
                 CATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCA 
               
               
                 CCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCA 
               
               
                 GGCTGCACCAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATG 
               
               
                 CGGCTGGCGGAGGAAGAGAAGCTT 
               
               
                   
               
               
                 SEQ ID NO: 79 (associated protein sequence from SEQ ID NO: 39) 
               
               
                 RSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRII 
               
               
                 QFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRL 
               
               
                 AEEEKL 
               
               
                   
               
               
                 SEQ ID NO: 40 is the relevant fragment of SEQ ID NO: 31 where potential in-frame stop 
               
               
                 codons are located in the second generation hybrid front half AP splice donor region (see, 
               
               
                 e.g. FIG. 42). 
               
               
                 CAACGCCTCAGGGCTGAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCA 
               
               
                 ATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGAGCTAGCCCC 
               
               
                   
               
               
                 SEQ ID NO: 41 is the relevant fragment of SEQ ID NO: 33 where potential in-frame stop 
               
               
                 codons are removed in the CMv1 hybrid front half AP splice donor region (see, e.g., FIG. 
               
               
                 42). 
               
               
                 CAACGCCTCAGGGCTGAGGTAAGTATCAAGGTTACAAGACAGGTTAACGGAGACCA 
               
               
                 ATTGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCAGCGCTAGCCCC 
               
               
                   
               
               
                 SEQ ID NO: 42 is the forward primer sequence of Gibson Primer Set 1 (reverse primer 
               
               
                 sequence is the reverse compliment of the forward primer (not shown))(see, e.g., FIG. 42). 
               
               
                 CCGCAGGCTGCACCAACGCCTCAGGGCTGAGGTAAGTATCAAGGTTACAAGACAG 
               
               
                 GTTAACGGAGACCAATTGAAACT 
               
               
                   
               
               
                 SEQ ID NO: 43 is the forward primer sequence of Gibson Primer Set 2 (reverse primer 
               
               
                 sequence is the reverse compliment of the forward primer (not shown))(see, e.g., FIG. 42). 
               
               
                 AACGGAGACCAATTGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCAG 
               
               
                 CGCTAGCCCCCGGGTGCGCGGCG 
               
               
                   
               
               
                 SEQ ID NO: 44 is the polynucleotide sequence of the CMv2.1 hybrid back half vector (i.e., 
               
               
                 AAV-APhead-APSA-hMYO7ACTex22-CMv2.1). 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCCCCGGGTGCGCGGCGTCGGTGGTGCCGGCG 
               
               
                 GGGGGCGCCAGGTCGCAGGCGGTGTAGGGCTCCAGGCAGGCGGCGAAGGCCATGACGTGC 
               
               
                 GCTATGAAGGTCTGCTCCTGCACGCCGTGAACCAGGTGCGCCTGCGGGCCGCGCGCGAACA 
               
               
                 CCGCCACGTCCTCGCCTGCGTGGGTCTCTTCGTCCAGGGGCACTGCGCACTGCTGCCGATAC 
               
               
                 TCGGGGCTCCCGCTCTCGCTCTCGGTAACATCCGGCCGGGCGCCGTCCTTGAGCACATAGCC 
               
               
                 TGGACCGTTTCTCTTAAGCGACGCATGCTCGCGATAGGCACCTATTGGTCTTACTGACATCC 
               
               
                 ACTTTGCCTTTCTCTCCACAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGA 
               
               
                 GGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCA 
               
               
                 AGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGA 
               
               
                 AGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGC 
               
               
                 CTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTG 
               
               
                 CCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAG 
               
               
                 ATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCT 
               
               
                 CTGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTA 
               
               
                 CACCCGGCGGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCA 
               
               
                 GCCCTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCA 
               
               
                 CACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTG 
               
               
                 GGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTC 
               
               
                 CCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTGCATTTGACTCTGAAAAAG 
               
               
                 AAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGCATGACGGGGAGTCCACAGTGCAG 
               
               
                 GGCAACAGCATGCTGGAGGACCGGCCCACCTCCAACCTGGAGAAGCTGCACTTCATCATCG 
               
               
                 GCAATGGCATCCTGCGGCCAGCACTCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCT 
               
               
                 GACCCACAACCCCTCCAAGAGCAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGG 
               
               
                 GCTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGTACCTGCGGAACTTCATCCACGGGGGCCCG 
               
               
                 CCCGGCTACGCCCCGTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTCAATGGGACACGGA 
               
               
                 CACAGCCGCCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCAATCATGTTGCC 
               
               
                 CGTGACATTCATGGATGGGACCACCAAGACCCTGCTGACGGACTCGGCAACCACGGCCAAG 
               
               
                 GAGCTCTGCAACGCGCTGGCCGACAAGATCTCTCTCAAGGACCGGTTCGGGTTCTCCCTCTA 
               
               
                 CATTGCCCTGTTTGACAAGGTGTCCTCCCTGGGCAGCGGCAGTGACCACGTCATGGACGCC 
               
               
                 ATCTCCCAGTGCGAGCAGTACGCCAAGGAGCAGGGCGCCCAGGAGCGCAACGCCCCCTGG 
               
               
                 AGGCTCTTCTTCCGCAAAGAGGTCTTCACGCCCTGGCACAGCCCCTCCGAGGACAACGTGG 
               
               
                 CCACCAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGA 
               
               
                 GAAGGAGGACGACCTGGCTGAGCTGGCCTCCCAGCAGTACTTTGTAGACTATGGCTCTGAG 
               
               
                 ATGATCCTGGAGCGCCTCCTGAACCTCGTGCCCACCTACATCCCCGACCGCGAGATCACGC 
               
               
                 CCCTGAAGACGCTGGAGAAGTGGGCCCAGCTGGCCATCGCCGCCCACAAGAAGGGGATTT 
               
               
                 ATGCCCAGAGGAGAACTGATGCCCAGAAGGTCAAAGAGGATGTGGTCAGTTATGCCCGCTT 
               
               
                 CAAGTGGCCCTTGCTCTTCTCCAGGTTTTATGAAGCCTACAAATTCTCAGGCCCCAGTCTCC 
               
               
                 CCAAGAACGACGTCATCGTGGCCGTCAACTGGACGGGTGTGTACTTTGTGGATGAGCAGGA 
               
               
                 GCAGGTACTTCTGGAGCTGTCCTTCCCAGAGATCATGGCCGTGTCCAGCAGCAGGGGAGCG 
               
               
                 AAAACGACGGCCCCCAGCTTCACGCTGGCCACCATCAAGGGGGACGAATACACCTTCACCT 
               
               
                 CCAGCAATGCTGAGGACATTCGTGACCTGGTGGTCACCTTCCTAGAGGGGCTCCGGAAGAG 
               
               
                 ATCTAAGTATGTTGTGGCCCTGCAGGATAACCCCAACCCCGCAGGCGAGGAGTCAGGCTTC 
               
               
                 CTCAGCTTTGCCAAGGGAGACCTCATCATCCTGGACCATGACACGGGCGAGCAGGTCATGA 
               
               
                 ACTCGGGCTGGGCCAACGGCATCAATGAGAGGACCAAGCAGCGTGGGGACTTCCCCACCG 
               
               
                 ACAGTGTGTACGTCATGCCCACTGTCACCATGCCACCGCGGGAGATTGTGGCCCTGGTCAC 
               
               
                 CATGACTCCCGATCAGAGGCAGGACGTTGTCCGGCTCTTGCAGCTGCGAACGGCGGAGCCC 
               
               
                 GAGGTGCGTGCCAAGCCCTACACGCTGGAGGAGTTTTCCTATGACTACTTCAGGCCCCCAC 
               
               
                 CCAAGCACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGAGGCAAGGACCGGCTGTGGA 
               
               
                 GCCACACGCGGGAACCGCTCAAGCAGGCGCTGCTCAAGAAGCTCCTGGGCAGTGAGGAGC 
               
               
                 TCTCGCAGGAGGCCTGCCTGGCCTTCATTGCTGTGCTCAAGTACATGGGCGACTACCCGTCC 
               
               
                 AAGAGGACACGCTCCGTCAACGAGCTCACCGACCAGATCTTTGAGGGTCCCCTGAAAGCCG 
               
               
                 AGCCCCTGAAGGACGAGGCATATGTGCAGATCCTGAAGCAGCTGACCGACAACCACATCA 
               
               
                 GGTACAGCGAGGAGCGGGGTTGGGAGCTGCTCTGGCTGTGCACGGGCCTTTTCCCACCCAG 
               
               
                 CAACATCCTCCTGCCCCACGTGCAGCGCTTCCTGCAGTCCCGAAAGCACTGCCCACTCGCCA 
               
               
                 TCGACTGCCTGCAACGGCTCCAGAAAGCCCTGAGAAACGGGTCCCGGAAGTACCCTCCGCA 
               
               
                 CCTGGTGGAGGTGGAGGCCATCCAGCACAAGACCACCCAGATTTTCCACAAAGTCTACTTC 
               
               
                 CCTGATGACACTGACGAGGCCTTCGAAGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCC 
               
               
                 AGAACATCGCCACCAGGCTGCTCCTCAAGTCCTCAGAGGGATTCAGCCTCTTTGTCAAAATT 
               
               
                 GCAGACAAGGTCATCAGCGTTCCTGAGAATGACTTCTTCTTTGACTTTGTTCGACACTTGAC 
               
               
                 AGACTGGATAAAGAAAGCTCGGCCCATCAAGGACGGAATTGTGCCCTCACTCACCTACCAG 
               
               
                 GTGTTCTTCATGAAGAAGCTGTGGACCACCACGGTGCCAGGGAAGGATCCCATGGCCGATT 
               
               
                 CCATCTTCCACTATTACCAGGAGTTGCCCAAGTATCTCCGAGGCTACCACAAGTGCACGCG 
               
               
                 GGAGGAGGTGCTGCAGCTGGGGGCGCTGATCTACAGGGTCAAGTTCGAGGAGGACAAGTC 
               
               
                 CTACTTCCCCAGCATCCCCAAGCTGCTGCGGGAGCTGGTGCCCCAGGACCTTATCCGGCAG 
               
               
                 GTCTCACCTGATGACTGGAAGCGGTCCATCGTCGCCTACTTCAACAAGCACGCAGGGAAGT 
               
               
                 CCAAGGAGGAGGCCAAGCTGGCCTTCCTGAAGCTCATCTTCAAGTGGCCCACCTTTGGCTC 
               
               
                 AGCCTTCTTCGAGGTGAAGCAAACTACGGAGCCAAACTTCCCTGAGATCCTCCTAATTGCC 
               
               
                 ATCAACAAGTATGGGGTCAGCCTCATCGATCCCAAAACGAAGGATATCCTCACCACTCATC 
               
               
                 CCTTCACCAAGATCTCCAACTGGAGCAGCGGCAACACCTACTTCCACATCACCATTGGGAA 
               
               
                 CTTGGTGCGCGGGAGCAAACTGCTCTGCGAGACGTCACTGGGCTACAAGATGGATGACCTC 
               
               
                 CTGACTTCCTACATTAGCCAGATGCTCACAGCCATGAGCAAACAGCGGGGCTCCAGGAGCG 
               
               
                 GCAAGAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGC 
               
               
                 CCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAA 
               
               
                 TGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGG 
               
               
                 CAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATTTAATTAAGCATGCTGGG 
               
               
                 GAGAGATCTGAGGAAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCG 
               
               
                 CTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAG 
               
               
                 TGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 45 is the portion of overlapping sequence from the original overlap vectors. 
               
               
                 CAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCCAGAGGCACTGGCGGGGT 
               
               
                 CACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGC 
               
               
                 ACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCA 
               
               
                 GGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCA 
               
               
                 CCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGA 
               
               
                 GTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGGAAGAGAAGCTTCGGAA 
               
               
                 GGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTGG 
               
               
                 CCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGA 
               
               
                 AGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACA 
               
               
                 TGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCA 
               
               
                 GGCACCTAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCT 
               
               
                 GGATGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGCC 
               
               
                 AAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACCCGGCGGCCACTCA 
               
               
                 AACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGAT 
               
               
                 CACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGAT 
               
               
                 GGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTGGGCAAGAAGACGTAC 
               
               
                 AAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCAGAAG 
               
               
                 AAGAGCAGTGTGAGGCACAAGCTGGTGCATTTGACTCTGAAAAAGAAGTCCAAGCTCACA 
               
               
                 GAGGAGGTGACCAAGAGGCTGCATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTG 
               
               
                 GAGGACCGGCCCACCTCCAACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGC 
               
               
                 GGCCAGCACTCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTC 
               
               
                 CAAGAGCAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCT 
               
               
                 CCGAGAAGTTTGTCAAGTACCTGCGGAACTTC 
               
               
                   
               
               
                 SEQ ID NO: 46 is the polynucleotide sequence of the CMv3 hybrid front half vector (i.e., 
               
               
                 AAV-smCBA-hMYO7A-NT-Ex21-APSD-APhead-CMv3)(pairs with CMv2 back half 
               
               
                 vector). 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCAATTCGGTACCCTAGTTATTAATAGTAATCA 
               
               
                 ATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA 
               
               
                 TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC 
               
               
                 CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAAC 
               
               
                 TGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG 
               
               
                 ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGG 
               
               
                 CAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTT 
               
               
                 CACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTT 
               
               
                 TGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGG 
               
               
                 CGAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGC 
               
               
                 TCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGC 
               
               
                 GCGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCG 
               
               
                 CGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCC 
               
               
                 CTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC 
               
               
                 GTGAAAGCCTTGAGGGGCTCCGGGAGCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCT 
               
               
                 TTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAA 
               
               
                 TTCTAGCGGCCGCCACCATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAG 
               
               
                 ATTGGGGCAGGAGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAG 
               
               
                 GTCCAGGTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACA 
               
               
                 TCAAGCCTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCT 
               
               
                 CAACGAGGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACG 
               
               
                 TATACGGGCTCCATCCTGGTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGA 
               
               
                 GCACATCCGCCAGTATACCAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATT 
               
               
                 GCTGACAACTGCTACTTCAACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTG 
               
               
                 GGGAATCTGGGGCCGGGAAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCA 
               
               
                 TCAGTGGGCAGCACTCGTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGC 
               
               
                 ATTTGGGAATGCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGAC 
               
               
                 ATCCACTTCAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAA 
               
               
                 AAGTCACGTGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGC 
               
               
                 TGGAGGGTATGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACA 
               
               
                 ACTACTTGGCCATGGGTAACTGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGC 
               
               
                 CAACATCCGCTCCGCCATGAAGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCG 
               
               
                 AAGCTCCTGGCTGCCATCCTGCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAA 
               
               
                 ACCTGGATGCCTGTGAGGTTCTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAG 
               
               
                 GTGAACCCCCCAGACCTGATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGA 
               
               
                 CGGTGTCCACCCCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGG 
               
               
                 GATCTACGGGCGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCT 
               
               
                 CCCTCCCAGGATGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGA 
               
               
                 GAACTTTGCTGTGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGC 
               
               
                 AGTTCTTTGTGCGGCACGTGTTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGA 
               
               
                 CTGGCTGCACATCGAGTTCACTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCC 
               
               
                 ATGAACATCATCTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCA 
               
               
                 TGTTACACAAGCTGAACTCCCAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAA 
               
               
                 CCATGAGACCCAGTTTGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCT 
               
               
                 TCCTGGAGAAGAACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAG 
               
               
                 GAACAAGTTCATCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAG 
               
               
                 CGCTCGCCCACACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGG 
               
               
                 GTGCCTGCCAGCCCTTCTTTGTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTG 
               
               
                 TTCGACCGGCACCTGTGCGTGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAA 
               
               
                 TCCGCCGAGCTGGCTACCCCATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTG 
               
               
                 CTGCTGCCAGGTGTGAAGCCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCA 
               
               
                 TGGCTGAGGCTGTGCTGGGCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCT 
               
               
                 GAAGGACCACCATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGT 
               
               
                 CATCCTCCTTCAGAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAG 
               
               
                 AACGCTGCCACACTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGG 
               
               
                 CTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGC 
               
               
                 AGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTG 
               
               
                 CGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCA 
               
               
                 TGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGAGGTAAGTATCAAGGTTACAAGACA 
               
               
                 GGTTAACGGAGACCAATTGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCAGC 
               
               
                 GCTAGCCCCCGGGTGCGCGGCGTCGGTGGTGCCGGCGGGGGGCGCCAGGTCGCAGGCGGT 
               
               
                 GTAGGGCTCCAGGCAGGCGGCGAAGGCCATGACGTGCGCTATGAAGGTCTGCTCCTGCACG 
               
               
                 CCGTGAACCAGGTGCGCCTGCGGGCCGCGCGCGAACACCGCCACGTCCTCGCCTGCGTGGG 
               
               
                 TCTCTTCGTCCAGGGGCACTGCGCACTGCTGCCGATACTCGGGGCTCCCGCTCTCGCTCTCG 
               
               
                 GTAACATCCGGCCGGGCGCCGTCCTTGAGCACATAGCCTGGACCGTTTCGTCGACGGATCC 
               
               
                 GCATGCTGGGGAGAGATCTGAGGAAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCG 
               
               
                 CGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGG 
               
               
                 GCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 47 is the polynucleotide sequence of the CMv2.1 hybrid back half vector, 
               
               
                 containing the HA tag (i.e., AAV-APhead-APSA-hMYO7ACTex22-CMv2.1.HA)(pairs 
               
               
                 with CMv2 front half vector). 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCCCCGGGTGCGCGGCGTCGGTGGTGCCGGCG 
               
               
                 GGGGGCGCCAGGTCGCAGGCGGTGTAGGGCTCCAGGCAGGCGGCGAAGGCCATGACGTGC 
               
               
                 GCTATGAAGGTCTGCTCCTGCACGCCGTGAACCAGGTGCGCCTGCGGGCCGCGCGCGAACA 
               
               
                 CCGCCACGTCCTCGCCTGCGTGGGTCTCTTCGTCCAGGGGCACTGCGCACTGCTGCCGATAC 
               
               
                 TCGGGGCTCCCGCTCTCGCTCTCGGTAACATCCGGCCGGGCGCCGTCCTTGAGCACATAGCC 
               
               
                 TGGACCGTTTCTCTTAAGCGACGCATGCTCGCGATAGGCACCTATTGGTCTTACTGACATCC 
               
               
                 ACTTTGCCTTTCTCTCCACAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGA 
               
               
                 GGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCA 
               
               
                 AGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGA 
               
               
                 AGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGC 
               
               
                 CTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTG 
               
               
                 CCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAG 
               
               
                 ATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCT 
               
               
                 CTGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTA 
               
               
                 CACCCGGCGGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCA 
               
               
                 GCCCTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCA 
               
               
                 CACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTG 
               
               
                 GGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTC 
               
               
                 CCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTGCATTTGACTCTGAAAAAG 
               
               
                 AAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGCATGACGGGGAGTCCACAGTGCAG 
               
               
                 GGCAACAGCATGCTGGAGGACCGGCCCACCTCCAACCTGGAGAAGCTGCACTTCATCATCG 
               
               
                 GCAATGGCATCCTGCGGCCAGCACTCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCT 
               
               
                 GACCCACAACCCCTCCAAGAGCAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGG 
               
               
                 GCTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGTACCTGCGGAACTTCATCCACGGGGGCCCG 
               
               
                 CCCGGCTACGCCCCGTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTCAATGGGACACGGA 
               
               
                 CACAGCCGCCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCAATCATGTTGCC 
               
               
                 CGTGACATTCATGGATGGGACCACCAAGACCCTGCTGACGGACTCGGCAACCACGGCCAAG 
               
               
                 GAGCTCTGCAACGCGCTGGCCGACAAGATCTCTCTCAAGGACCGGTTCGGGTTCTCCCTCTA 
               
               
                 CATTGCCCTGTTTGACAAGGTGTCCTCCCTGGGCAGCGGCAGTGACCACGTCATGGACGCC 
               
               
                 ATCTCCCAGTGCGAGCAGTACGCCAAGGAGCAGGGCGCCCAGGAGCGCAACGCCCCCTGG 
               
               
                 AGGCTCTTCTTCCGCAAAGAGGTCTTCACGCCCTGGCACAGCCCCTCCGAGGACAACGTGG 
               
               
                 CCACCAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGA 
               
               
                 GAAGGAGGACGACCTGGCTGAGCTGGCCTCCCAGCAGTACTTTGTAGACTATGGCTCTGAG 
               
               
                 ATGATCCTGGAGCGCCTCCTGAACCTCGTGCCCACCTACATCCCCGACCGCGAGATCACGC 
               
               
                 CCCTGAAGACGCTGGAGAAGTGGGCCCAGCTGGCCATCGCCGCCCACAAGAAGGGGATTT 
               
               
                 ATGCCCAGAGGAGAACTGATGCCCAGAAGGTCAAAGAGGATGTGGTCAGTTATGCCCGCTT 
               
               
                 CAAGTGGCCCTTGCTCTTCTCCAGGTTTTATGAAGCCTACAAATTCTCAGGCCCCAGTCTCC 
               
               
                 CCAAGAACGACGTCATCGTGGCCGTCAACTGGACGGGTGTGTACTTTGTGGATGAGCAGGA 
               
               
                 GCAGGTACTTCTGGAGCTGTCCTTCCCAGAGATCATGGCCGTGTCCAGCAGCAGGGGAGCG 
               
               
                 AAAACGACGGCCCCCAGCTTCACGCTGGCCACCATCAAGGGGGACGAATACACCTTCACCT 
               
               
                 CCAGCAATGCTGAGGACATTCGTGACCTGGTGGTCACCTTCCTAGAGGGGCTCCGGAAGAG 
               
               
                 ATCTAAGTATGTTGTGGCCCTGCAGGATAACCCCAACCCCGCAGGCGAGGAGTCAGGCTTC 
               
               
                 CTCAGCTTTGCCAAGGGAGACCTCATCATCCTGGACCATGACACGGGCGAGCAGGTCATGA 
               
               
                 ACTCGGGCTGGGCCAACGGCATCAATGAGAGGACCAAGCAGCGTGGGGACTTCCCCACCG 
               
               
                 ACAGTGTGTACGTCATGCCCACTGTCACCATGCCACCGCGGGAGATTGTGGCCCTGGTCAC 
               
               
                 CATGACTCCCGATCAGAGGCAGGACGTTGTCCGGCTCTTGCAGCTGCGAACGGCGGAGCCC 
               
               
                 GAGGTGCGTGCCAAGCCCTACACGCTGGAGGAGTTTTCCTATGACTACTTCAGGCCCCCAC 
               
               
                 CCAAGCACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGAGGCAAGGACCGGCTGTGGA 
               
               
                 GCCACACGCGGGAACCGCTCAAGCAGGCGCTGCTCAAGAAGCTCCTGGGCAGTGAGGAGC 
               
               
                 TCTCGCAGGAGGCCTGCCTGGCCTTCATTGCTGTGCTCAAGTACATGGGCGACTACCCGTCC 
               
               
                 AAGAGGACACGCTCCGTCAACGAGCTCACCGACCAGATCTTTGAGGGTCCCCTGAAAGCCG 
               
               
                 AGCCCCTGAAGGACGAGGCATATGTGCAGATCCTGAAGCAGCTGACCGACAACCACATCA 
               
               
                 GGTACAGCGAGGAGCGGGGTTGGGAGCTGCTCTGGCTGTGCACGGGCCTTTTCCCACCCAG 
               
               
                 CAACATCCTCCTGCCCCACGTGCAGCGCTTCCTGCAGTCCCGAAAGCACTGCCCACTCGCCA 
               
               
                 TCGACTGCCTGCAACGGCTCCAGAAAGCCCTGAGAAACGGGTCCCGGAAGTACCCTCCGCA 
               
               
                 CCTGGTGGAGGTGGAGGCCATCCAGCACAAGACCACCCAGATTTTCCACAAAGTCTACTTC 
               
               
                 CCTGATGACACTGACGAGGCCTTCGAAGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCC 
               
               
                 AGAACATCGCCACCAGGCTGCTCCTCAAGTCCTCAGAGGGATTCAGCCTCTTTGTCAAAATT 
               
               
                 GCAGACAAGGTCATCAGCGTTCCTGAGAATGACTTCTTCTTTGACTTTGTTCGACACTTGAC 
               
               
                 AGACTGGATAAAGAAAGCTCGGCCCATCAAGGACGGAATTGTGCCCTCACTCACCTACCAG 
               
               
                 GTGTTCTTCATGAAGAAGCTGTGGACCACCACGGTGCCAGGGAAGGATCCCATGGCCGATT 
               
               
                 CCATCTTCCACTATTACCAGGAGTTGCCCAAGTATCTCCGAGGCTACCACAAGTGCACGCG 
               
               
                 GGAGGAGGTGCTGCAGCTGGGGGCGCTGATCTACAGGGTCAAGTTCGAGGAGGACAAGTC 
               
               
                 CTACTTCCCCAGCATCCCCAAGCTGCTGCGGGAGCTGGTGCCCCAGGACCTTATCCGGCAG 
               
               
                 GTCTCACCTGATGACTGGAAGCGGTCCATCGTCGCCTACTTCAACAAGCACGCAGGGAAGT 
               
               
                 CCAAGGAGGAGGCCAAGCTGGCCTTCCTGAAGCTCATCTTCAAGTGGCCCACCTTTGGCTC 
               
               
                 AGCCTTCTTCGAGGTGAAGCAAACTACGGAGCCAAACTTCCCTGAGATCCTCCTAATTGCC 
               
               
                 ATCAACAAGTATGGGGTCAGCCTCATCGATCCCAAAACGAAGGATATCCTCACCACTCATC 
               
               
                 CCTTCACCAAGATCTCCAACTGGAGCAGCGGCAACACCTACTTCCACATCACCATTGGGAA 
               
               
                 CTTGGTGCGCGGGAGCAAACTGCTCTGCGAGACGTCACTGGGCTACAAGATGGATGACCTC 
               
               
                 CTGACTTCCTACATTAGCCAGATGCTCACAGCCATGAGCAAACAGCGGGGCTCCAGGAGCG 
               
               
                 GCAAGTACCCTTACGATGTACCGGATTACGCATGAAGAGCTCGCTGATCAGCCTCGACTGT 
               
               
                 GCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGG 
               
               
                 TGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGT 
               
               
                 GTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACA 
               
               
                 ATAGCAGGCATTTAATTAAGCATGCTGGGGAGAGATCTGAGGAAACCCCTAGTGATGGAGT 
               
               
                 TGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCG 
               
               
                 ACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 48 is the polynucleotide sequence of the minimized second-generation hybrid 
               
               
                 back half vector, containing the HA tag (i.e., AAV-APhead-APSA-hMYO7ACTex22.HA- 
               
               
                 MIN)(pairs with second generation front half hybrid vector). 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCCCCGGGTGCGCGGCGTCGGTGGTGCCGGCG 
               
               
                 GGGGGCGCCAGGTCGCAGGCGGTGTAGGGCTCCAGGCAGGCGGCGAAGGCCATGACGTGC 
               
               
                 GCTATGAAGGTCTGCTCCTGCACGCCGTGAACCAGGTGCGCCTGCGGGCCGCGCGCGAACA 
               
               
                 CCGCCACGTCCTCGCCTGCGTGGGTCTCTTCGTCCAGGGGCACTGCTGACTGCTGCCGATAC 
               
               
                 TCGGGGCTCCCGCTCTCGCTCTCGGTAACATCCGGCCGGGCGCCGTCCTTGAGCACATAGCC 
               
               
                 TGGACCGTTTCCTTAAGCGACGCATGCTCGCGATAGGCACCTATTGGTCTTACTGACATCCA 
               
               
                 CTTTGCCTTTCTCTCCACAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAG 
               
               
                 GAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAA 
               
               
                 GCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAA 
               
               
                 GGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGCC 
               
               
                 TGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGC 
               
               
                 CAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGA 
               
               
                 TGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTC 
               
               
                 TGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTAC 
               
               
                 ACCCGGCGGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAG 
               
               
                 CCCTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCAC 
               
               
                 ACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTGG 
               
               
                 GCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTCC 
               
               
                 CCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTGCATTTGACTCTGAAAAAGA 
               
               
                 AGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGCATGACGGGGAGTCCACAGTGCAGG 
               
               
                 GCAACAGCATGCTGGAGGACCGGCCCACCTCCAACCTGGAGAAGCTGCACTTCATCATCGG 
               
               
                 CAATGGCATCCTGCGGCCAGCACTCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCTG 
               
               
                 ACCCACAACCCCTCCAAGAGCAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGG 
               
               
                 CTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGTACCTGCGGAACTTCATCCACGGGGGCCCGC 
               
               
                 CCGGCTACGCCCCGTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTCAATGGGACACGGAC 
               
               
                 ACAGCCGCCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCAATCATGTTGCCC 
               
               
                 GTGACATTCATGGATGGGACCACCAAGACCCTGCTGACGGACTCGGCAACCACGGCCAAGG 
               
               
                 AGCTCTGCAACGCGCTGGCCGACAAGATCTCTCTCAAGGACCGGTTCGGGTTCTCCCTCTAC 
               
               
                 ATTGCCCTGTTTGACAAGGTGTCCTCCCTGGGCAGCGGCAGTGACCACGTCATGGACGCCA 
               
               
                 TCTCCCAGTGCGAGCAGTACGCCAAGGAGCAGGGCGCCCAGGAGCGCAACGCCCCCTGGA 
               
               
                 GGCTCTTCTTCCGCAAAGAGGTCTTCACGCCCTGGCACAGCCCCTCCGAGGACAACGTGGC 
               
               
                 CACCAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGAG 
               
               
                 AAGGAGGACGACCTGGCTGAGCTGGCCTCCCAGCAGTACTTTGTAGACTATGGCTCTGAGA 
               
               
                 TGATCCTGGAGCGCCTCCTGAACCTCGTGCCCACCTACATCCCCGACCGCGAGATCACGCCC 
               
               
                 CTGAAGACGCTGGAGAAGTGGGCCCAGCTGGCCATCGCCGCCCACAAGAAGGGGATTTAT 
               
               
                 GCCCAGAGGAGAACTGATGCCCAGAAGGTCAAAGAGGATGTGGTCAGTTATGCCCGCTTCA 
               
               
                 AGTGGCCCTTGCTCTTCTCCAGGTTTTATGAAGCCTACAAATTCTCAGGCCCCAGTCTCCCC 
               
               
                 AAGAACGACGTCATCGTGGCCGTCAACTGGACGGGTGTGTACTTTGTGGATGAGCAGGAGC 
               
               
                 AGGTACTTCTGGAGCTGTCCTTCCCAGAGATCATGGCCGTGTCCAGCAGCAGGGGAGCGAA 
               
               
                 AACGACGGCCCCCAGCTTCACGCTGGCCACCATCAAGGGGGACGAATACACCTTCACCTCC 
               
               
                 AGCAATGCTGAGGACATTCGTGACCTGGTGGTCACCTTCCTAGAGGGGCTCCGGAAGAGAT 
               
               
                 CTAAGTATGTTGTGGCCCTGCAGGATAACCCCAACCCCGCAGGCGAGGAGTCAGGCTTCCT 
               
               
                 CAGCTTTGCCAAGGGAGACCTCATCATCCTGGACCATGACACGGGCGAGCAGGTCATGAAC 
               
               
                 TCGGGCTGGGCCAACGGCATCAATGAGAGGACCAAGCAGCGTGGGGACTTCCCCACCGAC 
               
               
                 AGTGTGTACGTCATGCCCACTGTCACCATGCCACCGCGGGAGATTGTGGCCCTGGTCACCAT 
               
               
                 GACTCCCGATCAGAGGCAGGACGTTGTCCGGCTCTTGCAGCTGCGAACGGCGGAGCCCGAG 
               
               
                 GTGCGTGCCAAGCCCTACACGCTGGAGGAGTTTTCCTATGACTACTTCAGGCCCCCACCCAA 
               
               
                 GCACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGAGGCAAGGACCGGCTGTGGAGCCAC 
               
               
                 ACGCGGGAACCGCTCAAGCAGGCGCTGCTCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGC 
               
               
                 AGGAGGCCTGCCTGGCCTTCATTGCTGTGCTCAAGTACATGGGCGACTACCCGTCCAAGAG 
               
               
                 GACACGCTCCGTCAACGAGCTCACCGACCAGATCTTTGAGGGTCCCCTGAAAGCCGAGCCC 
               
               
                 CTGAAGGACGAGGCATATGTGCAGATCCTGAAGCAGCTGACCGACAACCACATCAGGTAC 
               
               
                 AGCGAGGAGCGGGGTTGGGAGCTGCTCTGGCTGTGCACGGGCCTTTTCCCACCCAGCAACA 
               
               
                 TCCTCCTGCCCCACGTGCAGCGCTTCCTGCAGTCCCGAAAGCACTGCCCACTCGCCATCGAC 
               
               
                 TGCCTGCAACGGCTCCAGAAAGCCCTGAGAAACGGGTCCCGGAAGTACCCTCCGCACCTGG 
               
               
                 TGGAGGTGGAGGCCATCCAGCACAAGACCACCCAGATTTTCCACAAAGTCTACTTCCCTGA 
               
               
                 TGACACTGACGAGGCCTTCGAAGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAAC 
               
               
                 ATCGCCACCAGGCTGCTCCTCAAGTCCTCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGA 
               
               
                 CAAGGTCATCAGCGTTCCTGAGAATGACTTCTTCTTTGACTTTGTTCGACACTTGACAGACT 
               
               
                 GGATAAAGAAAGCTCGGCCCATCAAGGACGGAATTGTGCCCTCACTCACCTACCAGGTGTT 
               
               
                 CTTCATGAAGAAGCTGTGGACCACCACGGTGCCAGGGAAGGATCCCATGGCCGATTCCATC 
               
               
                 TTCCACTATTACCAGGAGTTGCCCAAGTATCTCCGAGGCTACCACAAGTGCACGCGGGAGG 
               
               
                 AGGTGCTGCAGCTGGGGGCGCTGATCTACAGGGTCAAGTTCGAGGAGGACAAGTCCTACTT 
               
               
                 CCCCAGCATCCCCAAGCTGCTGCGGGAGCTGGTGCCCCAGGACCTTATCCGGCAGGTCTCA 
               
               
                 CCTGATGACTGGAAGCGGTCCATCGTCGCCTACTTCAACAAGCACGCAGGGAAGTCCAAGG 
               
               
                 AGGAGGCCAAGCTGGCCTTCCTGAAGCTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTC 
               
               
                 TTCGAGGTGAAGCAAACTACGGAGCCAAACTTCCCTGAGATCCTCCTAATTGCCATCAACA 
               
               
                 AGTATGGGGTCAGCCTCATCGATCCCAAAACGAAGGATATCCTCACCACTCATCCCTTCACC 
               
               
                 AAGATCTCCAACTGGAGCAGCGGCAACACCTACTTCCACATCACCATTGGGAACTTGGTGC 
               
               
                 GCGGGAGCAAACTGCTCTGCGAGACGTCACTGGGCTACAAGATGGATGACCTCCTGACTTC 
               
               
                 CTACATTAGCCAGATGCTCACAGCCATGAGCAAACAGCGGGGCTCCAGGAGCGGCAAGTA 
               
               
                 CCCTTACGATGTACCGGATTACGCATGAAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTA 
               
               
                 GTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTC 
               
               
                 CCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCT 
               
               
                 ATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGG 
               
               
                 CATTTAATTAAGCATGCTGGGGAGAGATCTGAGGAAACCCCTAGTGATGGAGTTGGCCACT 
               
               
                 CCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGG 
               
               
                 GCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 49 is the polynucleotide sequence of the minimized second-generation hybrid 
               
               
                 back half vector, not containing the HA tag (i.e., AAV-APhead-APSA-hMYO7ACTex22- 
               
               
                 MIN)(pairs with second generation front half hybrid vector). 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCTCAGATCTGGCGCGCCCCCCGGGTGCGCGGCGTCGGTGGTGCCGGCG 
               
               
                 GGGGGCGCCAGGTCGCAGGCGGTGTAGGGCTCCAGGCAGGCGGCGAAGGCCATGACGTGC 
               
               
                 GCTATGAAGGTCTGCTCCTGCACGCCGTGAACCAGGTGCGCCTGCGGGCCGCGCGCGAACA 
               
               
                 CCGCCACGTCCTCGCCTGCGTGGGTCTCTTCGTCCAGGGGCACTGCTGACTGCTGCCGATAC 
               
               
                 TCGGGGCTCCCGCTCTCGCTCTCGGTAACATCCGGCCGGGCGCCGTCCTTGAGCACATAGCC 
               
               
                 TGGACCGTTTCCTTAAGCGACGCATGCTCGCGATAGGCACCTATTGGTCTTACTGACATCCA 
               
               
                 CTTTGCCTTTCTCTCCACAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAG 
               
               
                 GAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAA 
               
               
                 GCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAA 
               
               
                 GGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGCC 
               
               
                 TGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGC 
               
               
                 CAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGA 
               
               
                 TGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTC 
               
               
                 TGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTAC 
               
               
                 ACCCGGCGGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAG 
               
               
                 CCCTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCAC 
               
               
                 ACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTGG 
               
               
                 GCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTCC 
               
               
                 CCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTGCATTTGACTCTGAAAAAGA 
               
               
                 AGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGCATGACGGGGAGTCCACAGTGCAGG 
               
               
                 GCAACAGCATGCTGGAGGACCGGCCCACCTCCAACCTGGAGAAGCTGCACTTCATCATCGG 
               
               
                 CAATGGCATCCTGCGGCCAGCACTCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCTG 
               
               
                 ACCCACAACCCCTCCAAGAGCAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGG 
               
               
                 CTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGTACCTGCGGAACTTCATCCACGGGGGCCCGC 
               
               
                 CCGGCTACGCCCCGTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTCAATGGGACACGGAC 
               
               
                 ACAGCCGCCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCAATCATGTTGCCC 
               
               
                 GTGACATTCATGGATGGGACCACCAAGACCCTGCTGACGGACTCGGCAACCACGGCCAAGG 
               
               
                 AGCTCTGCAACGCGCTGGCCGACAAGATCTCTCTCAAGGACCGGTTCGGGTTCTCCCTCTAC 
               
               
                 ATTGCCCTGTTTGACAAGGTGTCCTCCCTGGGCAGCGGCAGTGACCACGTCATGGACGCCA 
               
               
                 TCTCCCAGTGCGAGCAGTACGCCAAGGAGCAGGGCGCCCAGGAGCGCAACGCCCCCTGGA 
               
               
                 GGCTCTTCTTCCGCAAAGAGGTCTTCACGCCCTGGCACAGCCCCTCCGAGGACAACGTGGC 
               
               
                 CACCAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGAG 
               
               
                 AAGGAGGACGACCTGGCTGAGCTGGCCTCCCAGCAGTACTTTGTAGACTATGGCTCTGAGA 
               
               
                 TGATCCTGGAGCGCCTCCTGAACCTCGTGCCCACCTACATCCCCGACCGCGAGATCACGCCC 
               
               
                 CTGAAGACGCTGGAGAAGTGGGCCCAGCTGGCCATCGCCGCCCACAAGAAGGGGATTTAT 
               
               
                 GCCCAGAGGAGAACTGATGCCCAGAAGGTCAAAGAGGATGTGGTCAGTTATGCCCGCTTCA 
               
               
                 AGTGGCCCTTGCTCTTCTCCAGGTTTTATGAAGCCTACAAATTCTCAGGCCCCAGTCTCCCC 
               
               
                 AAGAACGACGTCATCGTGGCCGTCAACTGGACGGGTGTGTACTTTGTGGATGAGCAGGAGC 
               
               
                 AGGTACTTCTGGAGCTGTCCTTCCCAGAGATCATGGCCGTGTCCAGCAGCAGGGGAGCGAA 
               
               
                 AACGACGGCCCCCAGCTTCACGCTGGCCACCATCAAGGGGGACGAATACACCTTCACCTCC 
               
               
                 AGCAATGCTGAGGACATTCGTGACCTGGTGGTCACCTTCCTAGAGGGGCTCCGGAAGAGAT 
               
               
                 CTAAGTATGTTGTGGCCCTGCAGGATAACCCCAACCCCGCAGGCGAGGAGTCAGGCTTCCT 
               
               
                 CAGCTTTGCCAAGGGAGACCTCATCATCCTGGACCATGACACGGGCGAGCAGGTCATGAAC 
               
               
                 TCGGGCTGGGCCAACGGCATCAATGAGAGGACCAAGCAGCGTGGGGACTTCCCCACCGAC 
               
               
                 AGTGTGTACGTCATGCCCACTGTCACCATGCCACCGCGGGAGATTGTGGCCCTGGTCACCAT 
               
               
                 GACTCCCGATCAGAGGCAGGACGTTGTCCGGCTCTTGCAGCTGCGAACGGCGGAGCCCGAG 
               
               
                 GTGCGTGCCAAGCCCTACACGCTGGAGGAGTTTTCCTATGACTACTTCAGGCCCCCACCCAA 
               
               
                 GCACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGAGGCAAGGACCGGCTGTGGAGCCAC 
               
               
                 ACGCGGGAACCGCTCAAGCAGGCGCTGCTCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGC 
               
               
                 AGGAGGCCTGCCTGGCCTTCATTGCTGTGCTCAAGTACATGGGCGACTACCCGTCCAAGAG 
               
               
                 GACACGCTCCGTCAACGAGCTCACCGACCAGATCTTTGAGGGTCCCCTGAAAGCCGAGCCC 
               
               
                 CTGAAGGACGAGGCATATGTGCAGATCCTGAAGCAGCTGACCGACAACCACATCAGGTAC 
               
               
                 AGCGAGGAGCGGGGTTGGGAGCTGCTCTGGCTGTGCACGGGCCTTTTCCCACCCAGCAACA 
               
               
                 TCCTCCTGCCCCACGTGCAGCGCTTCCTGCAGTCCCGAAAGCACTGCCCACTCGCCATCGAC 
               
               
                 TGCCTGCAACGGCTCCAGAAAGCCCTGAGAAACGGGTCCCGGAAGTACCCTCCGCACCTGG 
               
               
                 TGGAGGTGGAGGCCATCCAGCACAAGACCACCCAGATTTTCCACAAAGTCTACTTCCCTGA 
               
               
                 TGACACTGACGAGGCCTTCGAAGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAAC 
               
               
                 ATCGCCACCAGGCTGCTCCTCAAGTCCTCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGA 
               
               
                 CAAGGTCATCAGCGTTCCTGAGAATGACTTCTTCTTTGACTTTGTTCGACACTTGACAGACT 
               
               
                 GGATAAAGAAAGCTCGGCCCATCAAGGACGGAATTGTGCCCTCACTCACCTACCAGGTGTT 
               
               
                 CTTCATGAAGAAGCTGTGGACCACCACGGTGCCAGGGAAGGATCCCATGGCCGATTCCATC 
               
               
                 TTCCACTATTACCAGGAGTTGCCCAAGTATCTCCGAGGCTACCACAAGTGCACGCGGGAGG 
               
               
                 AGGTGCTGCAGCTGGGGGCGCTGATCTACAGGGTCAAGTTCGAGGAGGACAAGTCCTACTT 
               
               
                 CCCCAGCATCCCCAAGCTGCTGCGGGAGCTGGTGCCCCAGGACCTTATCCGGCAGGTCTCA 
               
               
                 CCTGATGACTGGAAGCGGTCCATCGTCGCCTACTTCAACAAGCACGCAGGGAAGTCCAAGG 
               
               
                 AGGAGGCCAAGCTGGCCTTCCTGAAGCTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTC 
               
               
                 TTCGAGGTGAAGCAAACTACGGAGCCAAACTTCCCTGAGATCCTCCTAATTGCCATCAACA 
               
               
                 AGTATGGGGTCAGCCTCATCGATCCCAAAACGAAGGATATCCTCACCACTCATCCCTTCACC 
               
               
                 AAGATCTCCAACTGGAGCAGCGGCAACACCTACTTCCACATCACCATTGGGAACTTGGTGC 
               
               
                 GCGGGAGCAAACTGCTCTGCGAGACGTCACTGGGCTACAAGATGGATGACCTCCTGACTTC 
               
               
                 CTACATTAGCCAGATGCTCACAGCCATGAGCAAACAGCGGGGCTCCAGGAGCGGCAAGAG 
               
               
                 AGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCC 
               
               
                 CGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAA 
               
               
                 TTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAG 
               
               
                 CAAGGGGGAGGATTGGGAAGACAATAGCAGGCATTTAATTAAGCATGCTGGGGAGAGATC 
               
               
                 TAGGAAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGG 
               
               
                 CCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCG 
               
               
                 AGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 50 is the polynucleotide sequence of the third generation overlap front half 
               
               
                 vector (i.e., AAV-smCBA-hMYO7A-NTlong-v3). 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCAGATCTGGCGCGCCCAATTCGGTACCCTAGTTATTAATAGTAATCAAT 
               
               
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTG 
               
               
                 CCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGAC 
               
               
                 GGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCA 
               
               
                 GTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCA 
               
               
                 CTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTG 
               
               
                 TGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCG 
               
               
                 AGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCC 
               
               
                 GAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCG 
               
               
                 GCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGC 
               
               
                 CGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCCCTT 
               
               
                 CTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTG 
               
               
                 AAAGCCTTGAGGGGCTCCGGGAGCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTT 
               
               
                 TCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTC 
               
               
                 TAGCGGCCGCCACCATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAGATT 
               
               
                 GGGGCAGGAGTTCGACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAGGTC 
               
               
                 CAGGTGGTGGATGATGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACATCA 
               
               
                 AGCCTATGCACCCCACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCTCAA 
               
               
                 CGAGGCGGGCATCTTGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACGTATA 
               
               
                 CGGGCTCCATCCTGGTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGAGCAC 
               
               
                 ATCCGCCAGTATACCAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATTGCTG 
               
               
                 ACAACTGCTACTTCAACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTGGGGA 
               
               
                 ATCTGGGGCCGGGAAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCATCAGT 
               
               
                 GGGCAGCACTCGTGGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGCATTTG 
               
               
                 GGAATGCCAAGACCATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGACATCCA 
               
               
                 CTTCAACAAGCGGGGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAAAAGTC 
               
               
                 ACGTGTCTGTCGCCAGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGCTGGAG 
               
               
                 GGTATGAGTGAGGATCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACAACTACT 
               
               
                 TGGCCATGGGTAACTGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGCCAACAT 
               
               
                 CCGCTCCGCCATGAAGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCGAAGCTC 
               
               
                 CTGGCTGCCATCCTGCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAAACCTGG 
               
               
                 ATGCCTGTGAGGTTCTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAGGTGAAC 
               
               
                 CCCCCAGACCTGATGAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGACGGTGT 
               
               
                 CCACCCCACTGAGCAGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGGGATCTA 
               
               
                 CGGGCGGCTGTTCGTGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCTCCCTCC 
               
               
                 CAGGATGTGAAGAACTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGAGAACTT 
               
               
                 TGCTGTGAACAGCTTTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGCAGTTCT 
               
               
                 TTGTGCGGCACGTGTTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGACTGGCT 
               
               
                 GCACATCGAGTTCACTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCCATGAAC 
               
               
                 ATCATCTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCATGTTAC 
               
               
                 ACAAGCTGAACTCCCAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAACCATGA 
               
               
                 GACCCAGTTTGGCATCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCTTCCTGG 
               
               
                 AGAAGAACCGAGACACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAGGAACAA 
               
               
                 GTTCATCAAGCAGATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAGCGCTCG 
               
               
                 CCCACACTTAGCAGCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGGGTGCCT 
               
               
                 GCCAGCCCTTCTTTGTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTGTTCGAC 
               
               
                 CGGCACCTGTGCGTGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAATCCGCC 
               
               
                 GAGCTGGCTACCCCATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTGCTGCTG 
               
               
                 CCAGGTGTGAAGCCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCATGGCTG 
               
               
                 AGGCTGTGCTGGGCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCTGAAGGA 
               
               
                 CCACCATGACATGCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGTCATCCTC 
               
               
                 CTTCAGAAAGTCATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAGAACGCTG 
               
               
                 CCACACTGATCCAGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGGCTGATGC 
               
               
                 GTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCG 
               
               
                 CCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAG 
               
               
                 GCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGC 
               
               
                 CCGCAGGCTGCACCAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATG 
               
               
                 CGGCTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGA 
               
               
                 GGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGA 
               
               
                 GCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGG 
               
               
                 CCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGAC 
               
               
                 TTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGA 
               
               
                 GGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACGAGGAT 
               
               
                 GAGGAGGACCTCTCTGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAA 
               
               
                 CCACGCACTCCTACACCCGGCGGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGG 
               
               
                 TGACCAGCTGGCAGCCCTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTG 
               
               
                 AGCCCAAGTACCACACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGAT 
               
               
                 TTATGAGACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGG 
               
               
                 CGAGGCCCAGCTCCCCGAGGGCCAGTTAATTAAGCATGCTGGGGAGAGATCTGAACCCCTA 
               
               
                 GTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAA 
               
               
                 AGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGAG 
               
               
                 AGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 66 (only the N-myosin7A portion of SEQ ID NO: 50) 
               
               
                 ATGGTGATTCTTCAGCAGGGGGACCATGTGTGGATGGACCTGAGATTGGGGCAGGAGTTCG 
               
               
                 ACGTGCCCATCGGGGCGGTGGTGAAGCTCTGCGACTCTGGGCAGGTCCAGGTGGTGGATGA 
               
               
                 TGAAGACAATGAACACTGGATCTCTCCGCAGAACGCAACGCACATCAAGCCTATGCACCCC 
               
               
                 ACGTCGGTCCACGGCGTGGAGGACATGATCCGCCTGGGGGACCTCAACGAGGCGGGCATCT 
               
               
                 TGCGCAACCTGCTTATCCGCTACCGGGACCACCTCATCTACACGTATACGGGCTCCATCCTG 
               
               
                 GTGGCTGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGAGCACATCCGCCAGTATAC 
               
               
                 CAACAAGAAGATTGGGGAGATGCCCCCCCACATCTTTGCCATTGCTGACAACTGCTACTTC 
               
               
                 AACATGAAACGCAACAGCCGAGACCAGTGCTGCATCATCAGTGGGGAATCTGGGGCCGGG 
               
               
                 AAGACGGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCAGCCATCAGTGGGCAGCACTCGT 
               
               
                 GGATTGAGCAGCAGGTCTTGGAGGCCACCCCCATTCTGGAAGCATTTGGGAATGCCAAGAC 
               
               
                 CATCCGCAATGACAACTCAAGCCGTTTCGGAAAGTACATCGACATCCACTTCAACAAGCGG 
               
               
                 GGCGCCATCGAGGGCGCGAAGATTGAGCAGTACCTGCTGGAAAAGTCACGTGTCTGTCGCC 
               
               
                 AGGCCCTGGATGAAAGGAACTACCACGTGTTCTACTGCATGCTGGAGGGTATGAGTGAGGA 
               
               
                 TCAGAAGAAGAAGCTGGGCTTGGGCCAGGCCTCTGACTACAACTACTTGGCCATGGGTAAC 
               
               
                 TGCATAACCTGTGAGGGCCGGGTGGACAGCCAGGAGTACGCCAACATCCGCTCCGCCATGA 
               
               
                 AGGTGCTCATGTTCACTGACACCGAGAACTGGGAGATCTCGAAGCTCCTGGCTGCCATCCT 
               
               
                 GCACCTGGGCAACCTGCAGTATGAGGCACGCACATTTGAAAACCTGGATGCCTGTGAGGTT 
               
               
                 CTCTTCTCCCCATCGCTGGCCACAGCTGCATCCCTGCTTGAGGTGAACCCCCCAGACCTGAT 
               
               
                 GAGCTGCCTGACTAGCCGCACCCTCATCACCCGCGGGGAGACGGTGTCCACCCCACTGAGC 
               
               
                 AGGGAACAGGCACTGGACGTGCGCGACGCCTTCGTAAAGGGGATCTACGGGCGGCTGTTCG 
               
               
                 TGTGGATTGTGGACAAGATCAACGCAGCAATTTACAAGCCTCCCTCCCAGGATGTGAAGAA 
               
               
                 CTCTCGCAGGTCCATCGGCCTCCTGGACATCTTTGGGTTTGAGAACTTTGCTGTGAACAGCT 
               
               
                 TTGAGCAGCTCTGCATCAACTTCGCCAATGAGCACCTGCAGCAGTTCTTTGTGCGGCACGTG 
               
               
                 TTCAAGCTGGAGCAGGAGGAATATGACCTGGAGAGCATTGACTGGCTGCACATCGAGTTCA 
               
               
                 CTGACAACCAGGATGCCCTGGACATGATTGCCAACAAGCCCATGAACATCATCTCCCTCAT 
               
               
                 CGATGAGGAGAGCAAGTTCCCCAAGGGCACAGACACCACCATGTTACACAAGCTGAACTCC 
               
               
                 CAGCACAAGCTCAACGCCAACTACATCCCCCCCAAGAACAACCATGAGACCCAGTTTGGCA 
               
               
                 TCAACCATTTTGCAGGCATCGTCTACTATGAGACCCAAGGCTTCCTGGAGAAGAACCGAGA 
               
               
                 CACCCTGCATGGGGACATTATCCAGCTGGTCCACTCCTCCAGGAACAAGTTCATCAAGCAG 
               
               
                 ATCTTCCAGGCCGATGTCGCCATGGGCGCCGAGACCAGGAAGCGCTCGCCCACACTTAGCA 
               
               
                 GCCAGTTCAAGCGGTCACTGGAGCTGCTGATGCGCACGCTGGGTGCCTGCCAGCCCTTCTTT 
               
               
                 GTGCGATGCATCAAGCCCAATGAGTTCAAGAAGCCCATGCTGTTCGACCGGCACCTGTGCG 
               
               
                 TGCGCCAGCTGCGGTACTCAGGAATGATGGAGACCATCCGAATCCGCCGAGCTGGCTACCC 
               
               
                 CATCCGCTACAGCTTCGTAGAGTTTGTGGAGCGGTACCGTGTGCTGCTGCCAGGTGTGAAG 
               
               
                 CCGGCCTACAAGCAGGGCGACCTCCGCGGGACTTGCCAGCGCATGGCTGAGGCTGTGCTGG 
               
               
                 GCACCCACGATGACTGGCAGATAGGCAAAACCAAGATCTTTCTGAAGGACCACCATGACAT 
               
               
                 GCTGCTGGAAGTGGAGCGGGACAAAGCCATCACCGACAGAGTCATCCTCCTTCAGAAAGTC 
               
               
                 ATCCGGGGATTCAAAGACAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCC 
               
               
                 AGAGGCACTGGCGGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCT 
               
               
                 GCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAG 
               
               
                 CGCATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCG 
               
               
                 CCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCAC 
               
               
                 CAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGG 
               
               
                 AAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAAG 
               
               
                 CATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAAG 
               
               
                 GAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGCCT 
               
               
                 GTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGC 
               
               
                 CAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGA 
               
               
                 TGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTC 
               
               
                 TGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTAC 
               
               
                 ACCCGGCGGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAG 
               
               
                 CCCTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCAC 
               
               
                 ACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTGG 
               
               
                 GCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTCC 
               
               
                 CCGAGGGCCAG 
               
               
                   
               
               
                 SEQ ID NO: 65 (peptide encoded by the N-myosin7A portion of SEQ ID NO: 50) 
               
               
                 MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPT 
               
               
                 SVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIG 
               
               
                 EMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATP 
               
               
                 ILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCML 
               
               
                 EGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKL 
               
               
                 LAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLS 
               
               
                 REQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQL 
               
               
                 CINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPK 
               
               
                 GTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVH 
               
               
                 SSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLF 
               
               
                 DRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAE 
               
               
                 AVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLI 
               
               
                 QRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHR 
               
               
                 LWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKH 
               
               
                 QERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQ 
               
               
                 EGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLK 
               
               
                 QPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKREL 
               
               
                 QALQGEGEAQLPEGQ 
               
               
                   
               
               
                 SEQ ID NO: 51 is the polynucleotide sequence of the third generation overlap back half 
               
               
                 vector (i.e., AAV-hMYO7A-CTlong-v3.HA). 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGC 
               
               
                 GACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCC 
               
               
                 ATCACTAGGGGTTCAGATCTGGCGCGCCCTGGCGGAGGAAGAGAAGCTTCGGAAGGAGAT 
               
               
                 GAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCT 
               
               
                 GGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGG 
               
               
                 AGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGA 
               
               
                 CAAGATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCT 
               
               
                 AGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCA 
               
               
                 GCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGTTCG 
               
               
                 CGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACCCGGCGGCCACTCAAACAGCC 
               
               
                 ACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGATCACCATC 
               
               
                 CTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGATGGCAGTG 
               
               
                 AGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTGGGCAAGAAGACGTACAAGAGGG 
               
               
                 AGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCA 
               
               
                 GTGTGAGGCACAAGCTGGTGCATTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGT 
               
               
                 GACCAAGAGGCTGCATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGACCG 
               
               
                 GCCCACCTCCAACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAGCA 
               
               
                 CTCCGGGACGAGATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAAGAGCA 
               
               
                 GCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCTCCGAGAAG 
               
               
                 TTTGTCAAGTACCTGCGGAACTTCATCCACGGGGGCCCGCCCGGCTACGCCCCGTACTGTGA 
               
               
                 GGAGCGCCTGAGAAGGACCTTTGTCAATGGGACACGGACACAGCCGCCCAGCTGGCTGGA 
               
               
                 GCTGCAGGCCACCAAGTCCAAGAAGCCAATCATGTTGCCCGTGACATTCATGGATGGGACC 
               
               
                 ACCAAGACCCTGCTGACGGACTCGGCAACCACGGCCAAGGAGCTCTGCAACGCGCTGGCCG 
               
               
                 ACAAGATCTCTCTCAAGGACCGGTTCGGGTTCTCCCTCTACATTGCCCTGTTTGACAAGGTG 
               
               
                 TCCTCCCTGGGCAGCGGCAGTGACCACGTCATGGACGCCATCTCCCAGTGCGAGCAGTACG 
               
               
                 CCAAGGAGCAGGGCGCCCAGGAGCGCAACGCCCCCTGGAGGCTCTTCTTCCGCAAAGAGGT 
               
               
                 CTTCACGCCCTGGCACAGCCCCTCCGAGGACAACGTGGCCACCAACCTCATCTACCAGCAG 
               
               
                 GTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTGTGAGAAGGAGGACGACCTGGCTGAG 
               
               
                 CTGGCCTCCCAGCAGTACTTTGTAGACTATGGCTCTGAGATGATCCTGGAGCGCCTCCTGAA 
               
               
                 CCTCGTGCCCACCTACATCCCCGACCGCGAGATCACGCCCCTGAAGACGCTGGAGAAGTGG 
               
               
                 GCCCAGCTGGCCATCGCCGCCCACAAGAAGGGGATTTATGCCCAGAGGAGAACTGATGCCC 
               
               
                 AGAAGGTCAAAGAGGATGTGGTCAGTTATGCCCGCTTCAAGTGGCCCTTGCTCTTCTCCAG 
               
               
                 GTTTTATGAAGCCTACAAATTCTCAGGCCCCAGTCTCCCCAAGAACGACGTCATCGTGGCCG 
               
               
                 TCAACTGGACGGGTGTGTACTTTGTGGATGAGCAGGAGCAGGTACTTCTGGAGCTGTCCTTC 
               
               
                 CCAGAGATCATGGCCGTGTCCAGCAGCAGGGGAGCGAAAACGACGGCCCCCAGCTTCACG 
               
               
                 CTGGCCACCATCAAGGGGGACGAATACACCTTCACCTCCAGCAATGCTGAGGACATTCGTG 
               
               
                 ACCTGGTGGTCACCTTCCTAGAGGGGCTCCGGAAGAGATCTAAGTATGTTGTGGCCCTGCA 
               
               
                 GGATAACCCCAACCCCGCAGGCGAGGAGTCAGGCTTCCTCAGCTTTGCCAAGGGAGACCTC 
               
               
                 ATCATCCTGGACCATGACACGGGCGAGCAGGTCATGAACTCGGGCTGGGCCAACGGCATCA 
               
               
                 ATGAGAGGACCAAGCAGCGTGGGGACTTCCCCACCGACAGTGTGTACGTCATGCCCACTGT 
               
               
                 CACCATGCCACCGCGGGAGATTGTGGCCCTGGTCACCATGACTCCCGATCAGAGGCAGGAC 
               
               
                 GTTGTCCGGCTCTTGCAGCTGCGAACGGCGGAGCCCGAGGTGCGTGCCAAGCCCTACACGC 
               
               
                 TGGAGGAGTTTTCCTATGACTACTTCAGGCCCCCACCCAAGCACACGCTGAGCCGTGTCATG 
               
               
                 GTGTCCAAGGCCCGAGGCAAGGACCGGCTGTGGAGCCACACGCGGGAACCGCTCAAGCAG 
               
               
                 GCGCTGCTCAAGAAGCTCCTGGGCAGTGAGGAGCTCTCGCAGGAGGCCTGCCTGGCCTTCA 
               
               
                 TTGCTGTGCTCAAGTACATGGGCGACTACCCGTCCAAGAGGACACGCTCCGTCAACGAGCT 
               
               
                 CACCGACCAGATCTTTGAGGGTCCCCTGAAAGCCGAGCCCCTGAAGGACGAGGCATATGTG 
               
               
                 CAGATCCTGAAGCAGCTGACCGACAACCACATCAGGTACAGCGAGGAGCGGGGTTGGGAG 
               
               
                 CTGCTCTGGCTGTGCACGGGCCTTTTCCCACCCAGCAACATCCTCCTGCCCCACGTGCAGCG 
               
               
                 CTTCCTGCAGTCCCGAAAGCACTGCCCACTCGCCATCGACTGCCTGCAACGGCTCCAGAAA 
               
               
                 GCCCTGAGAAACGGGTCCCGGAAGTACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAGC 
               
               
                 ACAAGACCACCCAGATTTTCCACAAAGTCTACTTCCCTGATGACACTGACGAGGCCTTCGA 
               
               
                 AGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCACCAGGCTGCTCCTC 
               
               
                 AAGTCCTCAGAGGGATTCAGCCTCTTTGTCAAAATTGCAGACAAGGTCATCAGCGTTCCTG 
               
               
                 AGAATGACTTCTTCTTTGACTTTGTTCGACACTTGACAGACTGGATAAAGAAAGCTCGGCCC 
               
               
                 ATCAAGGACGGAATTGTGCCCTCACTCACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGA 
               
               
                 CCACCACGGTGCCAGGGAAGGATCCCATGGCCGATTCCATCTTCCACTATTACCAGGAGTT 
               
               
                 GCCCAAGTATCTCCGAGGCTACCACAAGTGCACGCGGGAGGAGGTGCTGCAGCTGGGGGC 
               
               
                 GCTGATCTACAGGGTCAAGTTCGAGGAGGACAAGTCCTACTTCCCCAGCATCCCCAAGCTG 
               
               
                 CTGCGGGAGCTGGTGCCCCAGGACCTTATCCGGCAGGTCTCACCTGATGACTGGAAGCGGT 
               
               
                 CCATCGTCGCCTACTTCAACAAGCACGCAGGGAAGTCCAAGGAGGAGGCCAAGCTGGCCTT 
               
               
                 CCTGAAGCTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTCGAGGTGAAGCAAACTA 
               
               
                 CGGAGCCAAACTTCCCTGAGATCCTCCTAATTGCCATCAACAAGTATGGGGTCAGCCTCATC 
               
               
                 GATCCCAAAACGAAGGATATCCTCACCACTCATCCCTTCACCAAGATCTCCAACTGGAGCA 
               
               
                 GCGGCAACACCTACTTCCACATCACCATTGGGAACTTGGTGCGCGGGAGCAAACTGCTCTG 
               
               
                 CGAGACGTCACTGGGCTACAAGATGGATGACCTCCTGACTTCCTACATTAGCCAGATGCTC 
               
               
                 ACAGCCATGAGCAAACAGCGGGGCTCCAGGAGCGGCAAGTACCCTTACGATGTACCGGATT 
               
               
                 ACGCATGAGGTACCAAGGGCGAATTCTGCAGTCGACTAGAGCTCGCTGATCAGCCTCGACT 
               
               
                 GTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAA 
               
               
                 GGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAG 
               
               
                 GTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGA 
               
               
                 CAATAGCAGGCATGCTGGGGAGAGATCTGAGGACTAGTCCGTCGACTGTTAATTAAGCATG 
               
               
                 CTGGGGAGAGATCTGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCG 
               
               
                 CTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAG 
               
               
                 TGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
               
                   
               
               
                 SEQ ID NO: 80 (hMYO7A c-term (e.g., AAV-hMYO7A-CTlong-v3.HA) 
               
               
                 CTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGC 
               
               
                 CGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCT 
               
               
                 GAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCC 
               
               
                 GCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTC 
               
               
                 AGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGAGG 
               
               
                 GCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACGAGGATGA 
               
               
                 GGAGGACCTCTCTGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAACC 
               
               
                 ACGCACTCCTACACCCGGCGGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTG 
               
               
                 ACCAGCTGGCAGCCCTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGA 
               
               
                 GCCCAAGTACCACACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATT 
               
               
                 TATGAGACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGC 
               
               
                 GAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTGCATTTG 
               
               
                 ACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGCATGACGGGGAG 
               
               
                 TCCACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACCTCCAACCTGGAGAAGCTGC 
               
               
                 ACTTCATCATCGGCAATGGCATCCTGCGGCCAGCACTCCGGGACGAGATCTACTGCCAGAT 
               
               
                 CAGCAAGCAGCTGACCCACAACCCCTCCAAGAGCAGCTATGCCCGGGGCTGGATTCTCGTG 
               
               
                 TCTCTCTGCGTGGGCTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGTACCTGCGGAACTTCAT 
               
               
                 CCACGGGGGCCCGCCCGGCTACGCCCCGTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTC 
               
               
                 AATGGGACACGGACACAGCCGCCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAG 
               
               
                 CCAATCATGTTGCCCGTGACATTCATGGATGGGACCACCAAGACCCTGCTGACGGACTCGG 
               
               
                 CAACCACGGCCAAGGAGCTCTGCAACGCGCTGGCCGACAAGATCTCTCTCAAGGACCGGTT 
               
               
                 CGGGTTCTCCCTCTACATTGCCCTGTTTGACAAGGTGTCCTCCCTGGGCAGCGGCAGTGACC 
               
               
                 ACGTCATGGACGCCATCTCCCAGTGCGAGCAGTACGCCAAGGAGCAGGGCGCCCAGGAGC 
               
               
                 GCAACGCCCCCTGGAGGCTCTTCTTCCGCAAAGAGGTCTTCACGCCCTGGCACAGCCCCTCC 
               
               
                 GAGGACAACGTGGCCACCAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGG 
               
               
                 AGTACAGGTGTGAGAAGGAGGACGACCTGGCTGAGCTGGCCTCCCAGCAGTACTTTGTAGA 
               
               
                 CTATGGCTCTGAGATGATCCTGGAGCGCCTCCTGAACCTCGTGCCCACCTACATCCCCGACC 
               
               
                 GCGAGATCACGCCCCTGAAGACGCTGGAGAAGTGGGCCCAGCTGGCCATCGCCGCCCACA 
               
               
                 AGAAGGGGATTTATGCCCAGAGGAGAACTGATGCCCAGAAGGTCAAAGAGGATGTGGTCA 
               
               
                 GTTATGCCCGCTTCAAGTGGCCCTTGCTCTTCTCCAGGTTTTATGAAGCCTACAAATTCTCA 
               
               
                 GGCCCCAGTCTCCCCAAGAACGACGTCATCGTGGCCGTCAACTGGACGGGTGTGTACTTTG 
               
               
                 TGGATGAGCAGGAGCAGGTACTTCTGGAGCTGTCCTTCCCAGAGATCATGGCCGTGTCCAG 
               
               
                 CAGCAGGGGAGCGAAAACGACGGCCCCCAGCTTCACGCTGGCCACCATCAAGGGGGACGA 
               
               
                 ATACACCTTCACCTCCAGCAATGCTGAGGACATTCGTGACCTGGTGGTCACCTTCCTAGAGG 
               
               
                 GGCTCCGGAAGAGATCTAAGTATGTTGTGGCCCTGCAGGATAACCCCAACCCCGCAGGCGA 
               
               
                 GGAGTCAGGCTTCCTCAGCTTTGCCAAGGGAGACCTCATCATCCTGGACCATGACACGGGC 
               
               
                 GAGCAGGTCATGAACTCGGGCTGGGCCAACGGCATCAATGAGAGGACCAAGCAGCGTGGG 
               
               
                 GACTTCCCCACCGACAGTGTGTACGTCATGCCCACTGTCACCATGCCACCGCGGGAGATTGT 
               
               
                 GGCCCTGGTCACCATGACTCCCGATCAGAGGCAGGACGTTGTCCGGCTCTTGCAGCTGCGA 
               
               
                 ACGGCGGAGCCCGAGGTGCGTGCCAAGCCCTACACGCTGGAGGAGTTTTCCTATGACTACT 
               
               
                 TCAGGCCCCCACCCAAGCACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGAGGCAAGGA 
               
               
                 CCGGCTGTGGAGCCACACGCGGGAACCGCTCAAGCAGGCGCTGCTCAAGAAGCTCCTGGGC 
               
               
                 AGTGAGGAGCTCTCGCAGGAGGCCTGCCTGGCCTTCATTGCTGTGCTCAAGTACATGGGCG 
               
               
                 ACTACCCGTCCAAGAGGACACGCTCCGTCAACGAGCTCACCGACCAGATCTTTGAGGGTCC 
               
               
                 CCTGAAAGCCGAGCCCCTGAAGGACGAGGCATATGTGCAGATCCTGAAGCAGCTGACCGA 
               
               
                 CAACCACATCAGGTACAGCGAGGAGCGGGGTTGGGAGCTGCTCTGGCTGTGCACGGGCCTT 
               
               
                 TTCCCACCCAGCAACATCCTCCTGCCCCACGTGCAGCGCTTCCTGCAGTCCCGAAAGCACTG 
               
               
                 CCCACTCGCCATCGACTGCCTGCAACGGCTCCAGAAAGCCCTGAGAAACGGGTCCCGGAAG 
               
               
                 TACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAGCACAAGACCACCCAGATTTTCCACA 
               
               
                 AAGTCTACTTCCCTGATGACACTGACGAGGCCTTCGAAGTGGAGTCCAGCACCAAGGCCAA 
               
               
                 GGACTTCTGCCAGAACATCGCCACCAGGCTGCTCCTCAAGTCCTCAGAGGGATTCAGCCTCT 
               
               
                 TTGTCAAAATTGCAGACAAGGTCATCAGCGTTCCTGAGAATGACTTCTTCTTTGACTTTGTT 
               
               
                 CGACACTTGACAGACTGGATAAAGAAAGCTCGGCCCATCAAGGACGGAATTGTGCCCTCAC 
               
               
                 TCACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGACCACCACGGTGCCAGGGAAGGATCC 
               
               
                 CATGGCCGATTCCATCTTCCACTATTACCAGGAGTTGCCCAAGTATCTCCGAGGCTACCACA 
               
               
                 AGTGCACGCGGGAGGAGGTGCTGCAGCTGGGGGCGCTGATCTACAGGGTCAAGTTCGAGG 
               
               
                 AGGACAAGTCCTACTTCCCCAGCATCCCCAAGCTGCTGCGGGAGCTGGTGCCCCAGGACCT 
               
               
                 TATCCGGCAGGTCTCACCTGATGACTGGAAGCGGTCCATCGTCGCCTACTTCAACAAGCAC 
               
               
                 GCAGGGAAGTCCAAGGAGGAGGCCAAGCTGGCCTTCCTGAAGCTCATCTTCAAGTGGCCCA 
               
               
                 CCTTTGGCTCAGCCTTCTTCGAGGTGAAGCAAACTACGGAGCCAAACTTCCCTGAGATCCTC 
               
               
                 CTAATTGCCATCAACAAGTATGGGGTCAGCCTCATCGATCCCAAAACGAAGGATATCCTCA 
               
               
                 CCACTCATCCCTTCACCAAGATCTCCAACTGGAGCAGCGGCAACACCTACTTCCACATCACC 
               
               
                 ATTGGGAACTTGGTGCGCGGGAGCAAACTGCTCTGCGAGACGTCACTGGGCTACAAGATGG 
               
               
                 ATGACCTCCTGACTTCCTACATTAGCCAGATGCTCACAGCCATGAGCAAACAGCGGGGCTC 
               
               
                 CAGGAGCGGCAAG 
               
               
                   
               
               
                 SEQ ID NO: 81 (hMY07A c-term (e.g., AAV-hMYO7A-CTlong-v3.HA) 
               
               
                 LAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHE 
               
               
                 PVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSE 
               
               
                 YKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAM 
               
               
                 SDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEE 
               
               
                 VTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARG 
               
               
                 WILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSK 
               
               
                 KPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVM 
               
               
                 DAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCE 
               
               
                 KEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQR 
               
               
                 RTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLE 
               
               
                 LSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQD 
               
               
                 NPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMP 
               
               
                 PREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARG 
               
               
                 KDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPL 
               
               
                 KAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAID 
               
               
                 CLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIAT 
               
               
                 RLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLW 
               
               
                 TTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLREL 
               
               
                 VPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEI 
               
               
                 LLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLL 
               
               
                 TSYISQMLTAMSKQRGSRSGK 
               
            
           
         
       
     
     In some embodiments, the overlap polynucleotide vectors provided herein comprise a region of overlap that comprises a nucleotide sequence having 80%, 85%, 90%, 92.5%, 95%, 98%, or 99% sequence identity to any of the overlap sequences provided herein. In some embodiments, the overlapping regions comprises a nucleotide sequence having 80%, 85%, 90%, 92.5%, 95%, 98%, or 99% sequence identity to any one of SEQ ID NOs: 39 and 52-59. In some embodiments, the overlapping regions comprises a nucleotide sequence having 80%, 85%, 90%, 92.5%, 95%, 98%, or 99% sequence identity to any one of SEQ ID NOs: 39 and 53-59. In some embodiments, the overlapping regions (or polynucleotide sequence that overlaps) comprises a nucleotide sequence having 80%, 85%, 90%, 92.5%, 95%, 98%, or 99% sequence identity to SEQ ID NO: 39, 56 or 57. In some embodiments, the polynucleotide sequence that overlaps comprises a nucleotide sequence selected from any of SEQ ID NOs: 39 and 52-59. The overlap vectors provided herein may comprise a polynucleotide sequence that overlaps that differs from any of SEQ ID NOs: 39 and 52-59 by 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 10-15, 15-20, 20-25, or more than 25 nucleotides. In some embodiments, these overlap vectors contain two overlapping sequences disclosed herein, e.g., the mutually exclusive sequences SEQ ID NOs: 39 and 56, or the mutually exclusive sequences SEQ ID NOs: 39 and 57. 
     In exemplary embodiments, the polynucleotide sequence that overlaps comprises SEQ ID NO: 56. In some embodiments, the polynucleotide sequence that overlaps comprises SEQ ID NO: 57. In some embodiments, the polynucleotide sequence that overlaps comprises SEQ ID NO: 39. In some embodiments, the polynucleotide sequence that overlaps does not comprise SEQ ID NO: 52. 
     In some embodiments, the overlap polynucleotide vectors provided herein comprise a region of overlap (polynucleotide sequence that overlaps) that encodes a protein having an amino acid sequence comprising 95%, 98%, or 99% or greater sequence identity to any of SEQ ID NOs: 79 and 82-89. In exemplary embodiments, these vectors comprise a region of overlap (polynucleotide sequence that overlaps) that encodes a protein having the amino acid sequence of any one of SEQ ID NOs: 79 and 82-89. In exemplary embodiments, these vectors comprise a region of overlap (polynucleotide sequence that overlaps) that encodes a protein having the amino acid sequence of any one of SEQ ID NOs: 79 and 83-89. In some embodiments, the polynucleotide sequence that overlaps does not encode the amino acid sequence of SEQ ID NO: 82. 
     In various embodiments, the overlap polynucleotide vectors provided herein contain a region of overlap in the polypeptide coding sequence having a length of about 1365 bp, 1284 bp, 1027 bp, 1026 bp, 945 bp, 687 bp, 361 bp, 279 bp, or 20 bp. In some embodiments, overlap vectors contain a region of overlap of less than 1000 bp in length. In some embodiments, overlap vectors contain a region of overlap of less than 1365 bp. In some embodiments, overlap vectors contain a region of overlap of less than 700 bp. In some embodiments, the region of overlap has a length of between about 20 to 100 nucleotides, about 100 to 500 nucleotides, about 100 to 200 nucleotides, about 200 to 300 nucleotides, or about 300 to 400 nucleotides. 
     In various embodiments, the overlap polynucleotide vectors provided herein contain a region of overlap having a length of exactly 1365 bp, 1284 bp, 1027 bp, 1026 bp, 945 bp, 687 bp, 361 bp, 279 bp, or 20 bp. It will be understood that these regions of overlap may be mutually exclusive of one another in the coding sequence. In some embodiments, the overlap polynucleotide vectors contain one or more regions of overlap, e.g., contain two regions of overlap. In some embodiments, the overlap vectors contain two regions of overlap having lengths of 361 bp and 687 bp. 
     In exemplary embodiments, the overlap polynucleotide vectors provided herein contain a region of overlap having a length of 687 or 945 bp. In some embodiments, these vectors contain a region of overlapping MYO7A sequence having a length of 687 or 945 bp. In some embodiments, overlap vectors contain a region of overlap having a length of 361 bp. 
     
       
         
           
               
               
            
               
                 1365 bp Overlap. (SEQ ID NO: 52) 
                   
               
               
                 CAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCCAGAGGCACTGGC 
               
               
                   
               
               
                 GGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCTGCGGCTG 
               
               
                   
               
               
                 CAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAGCG 
               
               
                   
               
               
                 CATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCA 
               
               
                   
               
               
                 CCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCA 
               
               
                   
               
               
                 GGCTGCACCAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATG 
               
               
                   
               
               
                 CGGCTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAG 
               
               
                   
               
               
                 GAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACG 
               
               
                   
               
               
                 CTGAGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGA 
               
               
                   
               
               
                 GCAGATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAG 
               
               
                   
               
               
                 ATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACC 
               
               
                   
               
               
                 TAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTG 
               
               
                   
               
               
                 GATGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATT 
               
               
                   
               
               
                 TGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACCCGGC 
               
               
                   
               
               
                 GGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCC 
               
               
                   
               
               
                 CTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTA 
               
               
                   
               
               
                 CCACACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATG 
               
               
                   
               
               
                 AGACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGG 
               
               
                   
               
               
                 CGAGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTG 
               
               
                   
               
               
                 CATTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGC 
               
               
                   
               
               
                 ATGACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACCTC 
               
               
                   
               
               
                 CAACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAGCACTCC 
               
               
                   
               
               
                 GGGACGAGATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAAGAG 
               
               
                   
               
               
                 CAGCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCTC 
               
               
                   
               
               
                 CGAGAAGTTTGTCAAGTACCTGCGGAACTTC 
               
               
                   
               
               
                 SEQ ID NO: 82 
               
               
                 (protein sequence that corresponds to SEQ ID NO: 52) 
               
               
                 RSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRII 
               
               
                   
               
               
                 QFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRL 
               
               
                   
               
               
                 AEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMER 
               
               
                   
               
               
                 ARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPL 
               
               
                   
               
               
                 PDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILR 
               
               
                   
               
               
                 FMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKS 
               
               
                   
               
               
                 SVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILR 
               
               
                   
               
               
                 PALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNF 
               
               
                   
               
               
                 1284 bp Overlap. (SEQ ID NO: 53) 
               
               
                 GGGCTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCT 
               
               
                   
               
               
                 GCACCAGCAGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCC 
               
               
                   
               
               
                 GCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGC 
               
               
                   
               
               
                 AGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGAG 
               
               
                   
               
               
                 TATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGGAAGAGAAGCTTC 
               
               
                   
               
               
                 GGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAAGCATCAGG 
               
               
                   
               
               
                 AGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAAGGA 
               
               
                   
               
               
                 GGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAG 
               
               
                   
               
               
                 CCTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGG 
               
               
                   
               
               
                 TGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGA 
               
               
                   
               
               
                 GGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACG 
               
               
                   
               
               
                 AGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTC 
               
               
                   
               
               
                 CAGGGGACAACCACGCACTCCTACACCCGGCGGCCACTCAAACAGCCACTGCTCTA 
               
               
                   
               
               
                 CCATGACGACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGATCACCATCCTCC 
               
               
                   
               
               
                 GCTTCATGGGGGACCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGATGGCAGT 
               
               
                   
               
               
                 GAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTGGGCAAGAAGACGTACA 
               
               
                   
               
               
                 AGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCA 
               
               
                   
               
               
                 GAAGAAGAGCAGTGTGAGGCACAAGCTGGTGCATTTGACTCTGAAAAAGAAGTCC 
               
               
                   
               
               
                 AAGCTCACAGAGGAGGTGACCAAGAGGCTGCATGACGGGGAGTCCACAGTGCAGG 
               
               
                   
               
               
                 GCAACAGCATGCTGGAGGACCGGCCCACCTCCAACCTGGAGAAGCTGCACTTCATC 
               
               
                   
               
               
                 ATCGGCAATGGCATCCTGCGGCCAGCACTCCGGGACGAGATCTACTGCCAGATCAG 
               
               
                   
               
               
                 CAAGCAGCTGACCCACAACCCCTCCAAGAGCAGCTATGCCCGGGGCTGGATTCTCG 
               
               
                   
               
               
                 TGTCTCTCTGCGTGGGCTGTTTCGCCCCCTCCGAGAAGTTTGTCAAGTACCTGCGGA 
               
               
                   
               
               
                 ACTTC 
               
               
                   
               
               
                 SEQ ID NO: 83 
               
               
                 (protein sequence that corresponds to SEQ ID NO: 53) 
               
               
                 GLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQ 
               
               
                   
               
               
                 AYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQER 
               
               
                   
               
               
                 LAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLP 
               
               
                   
               
               
                 GQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTH 
               
               
                   
               
               
                 SYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKI 
               
               
                   
               
               
                 YETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLH 
               
               
                   
               
               
                 DGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARG 
               
               
                   
               
               
                 WILVSLCVGCFAPSEKFVKYLRNF 
               
               
                   
               
               
                 1027 bp Overlap. (SEQ ID NO: 54) 
               
               
                 CAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCCAGAGGCACTGGC 
               
               
                   
               
               
                 GGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCTGCGGCTG 
               
               
                   
               
               
                 CAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAGCG 
               
               
                   
               
               
                 CATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCA 
               
               
                   
               
               
                 CCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCA 
               
               
                   
               
               
                 GGCTGCACCAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATG 
               
               
                   
               
               
                 CGGCTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAG 
               
               
                   
               
               
                 GAGGAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACG 
               
               
                   
               
               
                 CTGAGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGA 
               
               
                   
               
               
                 GCAGATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAG 
               
               
                   
               
               
                 ATGTTTGGCTTCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACC 
               
               
                   
               
               
                 TAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTG 
               
               
                   
               
               
                 GATGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATT 
               
               
                   
               
               
                 TGCCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACCCGGC 
               
               
                   
               
               
                 GGCCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCC 
               
               
                   
               
               
                 CTGGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTA 
               
               
                   
               
               
                 CCACACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATG 
               
               
                   
               
               
                 AGACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGG 
               
               
                   
               
               
                 CGAGGCCCAGCTCCCCGAGGGCCAG 
               
               
                   
               
               
                 SEQ ID NO: 84 
               
               
                 (protein sequence that corresponds to SEQ ID NO: 54) 
               
               
                 RSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRII 
               
               
                   
               
               
                 QFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRL 
               
               
                   
               
               
                 AEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMER 
               
               
                   
               
               
                 ARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPL 
               
               
                   
               
               
                 PDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILR 
               
               
                   
               
               
                 FMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQ 
               
               
                   
               
               
                 1026 bp Overlap. (SEQ ID NO: 55) 
               
               
                 CTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAG 
               
               
                   
               
               
                 GAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTG 
               
               
                   
               
               
                 AGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGC 
               
               
                   
               
               
                 AGATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGAT 
               
               
                   
               
               
                 GTTTGGCTTCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTA 
               
               
                   
               
               
                 GTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGA 
               
               
                   
               
               
                 TGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTG 
               
               
                   
               
               
                 CCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACCCGGCGG 
               
               
                   
               
               
                 CCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCCCT 
               
               
                   
               
               
                 GGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACC 
               
               
                   
               
               
                 ACACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAG 
               
               
                   
               
               
                 ACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCG 
               
               
                   
               
               
                 AGGCCCAGCTCCCCGAGGGCCAGAAGAAGAGCAGTGTGAGGCACAAGCTGGTGCA 
               
               
                   
               
               
                 TTTGACTCTGAAAAAGAAGTCCAAGCTCACAGAGGAGGTGACCAAGAGGCTGCAT 
               
               
                   
               
               
                 GACGGGGAGTCCACAGTGCAGGGCAACAGCATGCTGGAGGACCGGCCCACCTCCA 
               
               
                   
               
               
                 ACCTGGAGAAGCTGCACTTCATCATCGGCAATGGCATCCTGCGGCCAGCACTCCGG 
               
               
                   
               
               
                 GACGAGATCTACTGCCAGATCAGCAAGCAGCTGACCCACAACCCCTCCAAGAGCA 
               
               
                   
               
               
                 GCTATGCCCGGGGCTGGATTCTCGTGTCTCTCTGCGTGGGCTGTTTCGCCCCCTCCG 
               
               
                   
               
               
                 AGAAGTTTGTCAAGTACCTGCGGAACTTC 
               
               
                   
               
               
                 SEQ ID NO: 85 
               
               
                 (protein sequence that corresponds to SEQ ID NO: 55) 
               
               
                 LAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQME 
               
               
                   
               
               
                 RARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALP 
               
               
                   
               
               
                 LPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITIL 
               
               
                   
               
               
                 RFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKK 
               
               
                   
               
               
                 SSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGIL 
               
               
                   
               
               
                 RPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNF 
               
               
                   
               
               
                 945 bp Overlap. (SEQ ID NO: 56) 
               
               
                 GGGCTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCT 
               
               
                   
               
               
                 GCACCAGCAGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCC 
               
               
                   
               
               
                 GCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGC 
               
               
                   
               
               
                 AGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGAG 
               
               
                   
               
               
                 TATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGGAAGAGAAGCTTC 
               
               
                   
               
               
                 GGAAGGAGATGAGCGCCAAGAAGGCCAAGGAGGAGGCCGAGCGCAAGCATCAGG 
               
               
                   
               
               
                 AGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTGAGCGGGAGCTGAAGGAGAAGGA 
               
               
                   
               
               
                 GGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGCAGATGGAAAGGGCCCGCCATGAG 
               
               
                   
               
               
                 CCTGTCAATCACTCAGACATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGG 
               
               
                   
               
               
                 TGGCCTGCCAGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGA 
               
               
                   
               
               
                 GGGCGGAGGGAGATGGTGGAGGAGGACCTGGATGCAGCCCTGCCCCTGCCTGACG 
               
               
                   
               
               
                 AGGATGAGGAGGACCTCTCTGAGTATAAATTTGCCAAGTTCGCGGCCACCTACTTC 
               
               
                   
               
               
                 CAGGGGACAACCACGCACTCCTACACCCGGCGGCCACTCAAACAGCCACTGCTCTA 
               
               
                   
               
               
                 CCATGACGACGAGGGTGACCAGCTGGCAGCCCTGGCGGTCTGGATCACCATCCTCC 
               
               
                   
               
               
                 GCTTCATGGGGGACCTCCCTGAGCCCAAGTACCACACAGCCATGAGTGATGGCAGT 
               
               
                   
               
               
                 GAGAAGATCCCTGTGATGACCAAGATTTATGAGACCCTGGGCAAGAAGACGTACA 
               
               
                   
               
               
                 AGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCAGCTCCCCGAGGGCCA 
               
               
                   
               
               
                 G 
               
               
                   
               
               
                 SEQ ID NO: 86 
               
               
                 (protein sequence that corresponds to SEQ ID NO: 56) 
               
               
                 GLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQ 
               
               
                   
               
               
                 AYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQER 
               
               
                   
               
               
                 LAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLP 
               
               
                   
               
               
                 GQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTH 
               
               
                   
               
               
                 SYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKI 
               
               
                   
               
               
                 YETLGKKTYKRELQALQGEGEAQLPEGQ 
               
               
                   
               
               
                 687 bp Overlap. (SEQ ID NO: 57) 
               
               
                 CTGGCGGAGGAAGAGAAGCTTCGGAAGGAGATGAGCGCCAAGAAGGCCAAGGAG 
               
               
                   
               
               
                 GAGGCCGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTGGCTCGTGAGGACGCTG 
               
               
                   
               
               
                 AGCGGGAGCTGAAGGAGAAGGAGGCCGCTCGGCGGAAGAAGGAGCTCCTGGAGC 
               
               
                   
               
               
                 AGATGGAAAGGGCCCGCCATGAGCCTGTCAATCACTCAGACATGGTGGACAAGAT 
               
               
                   
               
               
                 GTTTGGCTTCCTGGGGACTTCAGGTGGCCTGCCAGGCCAGGAGGGCCAGGCACCTA 
               
               
                   
               
               
                 GTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAGGAGGACCTGGA 
               
               
                   
               
               
                 TGCAGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTCTCTGAGTATAAATTTG 
               
               
                   
               
               
                 CCAAGTTCGCGGCCACCTACTTCCAGGGGACAACCACGCACTCCTACACCCGGCGG 
               
               
                   
               
               
                 CCACTCAAACAGCCACTGCTCTACCATGACGACGAGGGTGACCAGCTGGCAGCCCT 
               
               
                   
               
               
                 GGCGGTCTGGATCACCATCCTCCGCTTCATGGGGGACCTCCCTGAGCCCAAGTACC 
               
               
                   
               
               
                 ACACAGCCATGAGTGATGGCAGTGAGAAGATCCCTGTGATGACCAAGATTTATGAG 
               
               
                   
               
               
                 ACCCTGGGCAAGAAGACGTACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCG 
               
               
                   
               
               
                 AGGCCCAGCTCCCCGAGGGCCAG 
               
               
                   
               
               
                 SEQ ID NO: 87 
               
               
                 (protein sequence that corresponds to SEQ ID NO: 57) 
               
               
                 LAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQME 
               
               
                   
               
               
                 RARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALP 
               
               
                   
               
               
                 LPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITIL 
               
               
                   
               
               
                 RFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQ 
               
               
                   
               
               
                 361 bp Overlap (same as SEQ ID NO: 39) 
               
               
                 CAGGTCTAACTTTCTGAAGCTGAAGAACGCTGCCACACTGATCCAGAGGCACTGGC 
               
               
                   
               
               
                 GGGGTCACAACTGTAGGAAGAACTACGGGCTGATGCGTCTGGGCTTCCTGCGGCTG 
               
               
                   
               
               
                 CAGGCCCTGCACCGCTCCCGGAAGCTGCACCAGCAGTACCGCCTGGCCCGCCAGCG 
               
               
                   
               
               
                 CATCATCCAGTTCCAGGCCCGCTGCCGCGCCTATCTGGTGCGCAAGGCCTTCCGCCA 
               
               
                   
               
               
                 CCGCCTCTGGGCTGTGCTCACCGTGCAGGCCTATGCCCGGGGCATGATCGCCCGCA 
               
               
                   
               
               
                 GGCTGCACCAACGCCTCAGGGCTGAGTATCTGTGGCGCCTCGAGGCTGAGAAAATG 
               
               
                   
               
               
                 CGGCTGGCGGAGGAAGAGAAGCTT 
               
               
                   
               
               
                 279 bp Overlap (SEQ ID NO: 58) 
               
               
                 GGGCTGATGCGTCTGGGCTTCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCT 
               
               
                   
               
               
                 GCACCAGCAGTACCGCCTGGCCCGCCAGCGCATCATCCAGTTCCAGGCCCGCTGCC 
               
               
                   
               
               
                 GCGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCTGTGCTCACCGTGC 
               
               
                   
               
               
                 AGGCCTATGCCCGGGGCATGATCGCCCGCAGGCTGCACCAACGCCTCAGGGCTGAG 
               
               
                   
               
               
                 TATCTGTGGCGCCTCGAGGCTGAGAAAATGCGGCTGGCGGAGGAAGAGAAGCTT 
               
               
                   
               
               
                 SEQ ID NO: 88 
               
               
                 (protein sequence that corresponds to SEQ ID NO: 58) 
               
               
                 GLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQ 
               
               
                   
               
               
                 AYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKL 
               
               
                   
               
               
                 20 bp Overlap (SEQ ID NO: 59):  
               
               
                 TGGCGGAGGAAGAGAAGCTT 
               
               
                   
               
               
                 SEQ ID NO: 89 (protein sequence that corresponds to SEQ ID NO: 59):  
               
               
                 AEEEKL 
               
               
                   
               
            
           
         
       
     
     In some embodiments of the disclosed hybrid and overlap vectors, any of the disclosed front half vectors (5′ AAV) comprise a left inverted terminal repeat sequence that comprises a nucleotide sequence having at least 95% or 98% identity to SEQ ID NO: 60. In some embodiments of the disclosed hybrid and overlap vectors, any of the disclosed front half vectors comprise a left inverted terminal repeat sequence that comprises SEQ ID NO: 60. In some embodiments of the disclosed hybrid and overlap vectors, any of the disclosed back half vectors (3′ AAV) comprise a right inverted terminal repeat sequence that comprises SEQ ID NO: 61. In some embodiments of the disclosed hybrid and overlap vectors, any of the disclosed back half vectors comprise a right inverted terminal repeat sequence that comprises a nucleotide sequence having at least 95% or 98% identity to SEQ ID NO: 61. In various embodiments, any of the disclosed dual hybrid and overlap vector pairs comprise a left ITR sequence comprising SEQ ID NO: 60 and a right ITR sequence comprising SEQ ID NO: 61. 
     
       
         
           
               
            
               
                 Left ITR Sequence. 
               
               
                 (SEQ ID NO: 60) 
               
               
                 CTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCC 
               
               
                   
               
               
                 GGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCG 
               
               
                   
               
               
                 CAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTT 
               
               
                   
               
               
                 Right ITR Sequence. 
               
               
                 (SEQ ID NO: 61) 
               
               
                 AACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTC 
               
               
                   
               
               
                 ACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGC 
               
               
                   
               
               
                 GGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAG 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Overlap Region Information &amp; Sequences 
               
            
           
           
               
               
               
            
               
                 Overlap Fragment 
                 Position in hMYO7A cDNA 
                   
               
            
           
           
               
               
               
            
               
                 Name/Length: 
                 Start: 
                 End: 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1365 
                 2281 
                 3645 
               
               
                 1284 
                 2362 
                 3645 
               
               
                 1027 
                 2281 
                 3306 
               
               
                 1026 
                 2620 
                 3645 
               
               
                 945 
                 2362 
                 3306 
               
               
                 687 
                 2620 
                 3306 
               
               
                 361 
                 2280 
                 2640 
               
               
                 279 
                 2362 
                 2640 
               
               
                 20 
                 2621 
                 2640 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Select Examples 6 and 7 AAV Sequences and  
               
               
                 Components (amino acid (aa), nucleic acid  
               
               
                 (nt) 
               
            
           
           
               
               
               
            
               
                   
                 SEQUENCE 
                 FULL-LENGTH AAV SEQUENCE 
               
               
                   
                   
               
            
           
           
               
            
               
                 Myosin7A N-terminus 
               
            
           
           
               
               
               
            
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-noDimNT- 
               
               
                   
                 NO: 62 (aa) 
                 CMv1 (SEQ ID NO: 36) 
               
               
                   
                 SEQ ID  
                   
               
               
                   
                 NO: 63 (nt) 
                   
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-noDIM- 
               
               
                   
                 NO: 91 (aa) 
                 NTlong (SEQ ID NO: 37) 
               
               
                   
                 SEQ ID  
                   
               
               
                   
                 NO: 90 (nt) 
                   
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-NTlong- 
               
               
                   
                 NO: 65 (aa) 
                 v3 (SEQ ID NO: 50) 
               
               
                   
                 SEQ ID  
                   
               
               
                   
                 NO: 66 (nt) 
                   
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-NT-Ex21- 
               
               
                   
                 NO: 74 (aa) 
                 APSD-APhead (SEQ ID NO: 31) 
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-NT-Ex21- 
               
               
                   
                 NO: 73 (nt) 
                 APSD-APhead CMv1  
               
               
                   
                   
                 (SEQ ID NO: 33) 
               
               
                   
                   
                 AAV-smCBA-hMYO7A-NT-Ex21- 
               
               
                   
                   
                 APSD-APhead CMv2  
               
               
                   
                   
                 (SEQ ID NO: 34) 
               
               
                   
                   
                 AAV-smCBA-hMYO7A- NT-Ex21- 
               
               
                   
                   
                 APSD-APhead-CMv3  
               
               
                   
                   
                 (SEQ ID NO: 46) 
               
               
                   
                   
               
            
           
           
               
            
               
                 Myosin7A C-terminus 
               
            
           
           
               
               
               
            
               
                   
                 SEQ ID  
                 AAV-hMYO7A-CTlong-v2.HA  
               
               
                   
                 NO: 78 (aa) 
                 (SEQ ID NO: 38) 
               
               
                   
                 SEQ ID  
                 AAV-hMYO7A-CTlong-v3.HA 
               
               
                   
                 NO: 77 (nt) 
                 (SEQ ID NO: 51) 
               
               
                   
                 SEQ ID  
                   
               
               
                   
                 NO: 81 (aa) 
                   
               
               
                   
                 SEQ ID  
                   
               
               
                   
                 NO: 80 (nt) 
                   
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-APhead-APSA-ex22hMYO7A- 
               
               
                   
                 NO: 76 (aa) 
                 CT.HA (SEQ ID NO: 32) 
               
               
                   
                 SEQ ID  
                 AAV-APhead-APSA-ex22hMYO7A- 
               
               
                   
                 NO: 75 (nt) 
                 CT.HA-CMv2 (SEQ ID NO: 35) 
               
               
                   
                   
                 AAV-APhead-APSA-hMYO7ACTex22- 
               
               
                   
                   
                 CMv2.1 (SEQ ID NO: 44) 
               
               
                   
                   
                 AAV-APhead-APSA-hMYO7ACTex22- 
               
               
                   
                   
                 CMv2.1.HA (SEQ ID NO: 47) 
               
               
                   
                   
                 AAV-APhead-APSA-hMYO7ACTex  
               
               
                   
                   
                 22.HA-MIN (SEQ ID NO: 48) 
               
               
                   
                   
                 AAV-APhead-APSA-hMYO7ACTex22- 
               
               
                   
                   
                 MIN (SEQ ID NO: 49) 
               
               
                   
                   
               
            
           
           
               
            
               
                 Myosin7A overlap 
               
            
           
           
               
               
               
            
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-noDIM- 
               
               
                   
                 NO: 79 (aa) 
                 NTlong (SEQ ID NO: 37) + AAV- 
               
               
                   
                 SEQ ID  
                 hMYO7A-CTlong.HA (SEQ ID NO: 2) 
               
               
                   
                 NO: 39 (nt) 
                 AAV-smCBA-hMYO7A-noDIM-NTlong- 
               
               
                   
                   
                 CMv1 (SEQ ID NO: 36) + AAV- 
               
               
                   
                   
                 hMYO7A-CTlong.HA (SEQ ID NO: 2) 
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-NTlong  
               
               
                   
                 NO: 82 (aa) 
                 (SEQ ID NO: 1) + AAV-hMYO7A- 
               
               
                   
                 SEQ ID  
                 CTlong.HA (SEQ ID NO: 2) 
               
               
                   
                 NO: 52 (nt) 
                   
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-NTlong  
               
               
                   
                 NO: 83 (aa) 
                 (SEQ ID NO: 1) + AAV-hMYO7A- 
               
               
                   
                 SEQ ID  
                 CTlong-v2.HA (SEQ ID NO: 38) 
               
               
                   
                 NO: 53 (nt) 
                   
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-NTlong-v3  
               
               
                   
                 NO: 84 (aa) 
                 (SEQ ID NO: 50) + AAV-hMYO7A- 
               
               
                   
                 SEQ ID  
                 CTlong.HA (SEQ ID NO: 2) 
               
               
                   
                 NO: 54 (nt) 
                   
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-NTlong  
               
               
                   
                 NO: 85 (aa) 
                 (SEQ ID NO: 1) + AAV-hMYO7A- 
               
               
                   
                 SEQ ID  
                 CTlong-v3.HA (SEQ ID NO: 51) 
               
               
                   
                 NO: 55 (nt) 
                   
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-NTlong-v3  
               
               
                   
                 NO: 86 (aa) 
                 (SEQ ID NO: 50) + AAV-hMYO7A- 
               
               
                   
                 SEQ ID  
                 CTlong-v2.HA (SEQ ID NO: 38) 
               
               
                   
                 NO: 56 (nt) 
                   
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-NTlong-v3  
               
               
                   
                 NO: 87 (aa) 
                 (SEQ ID NO: 50) + AAV-hMYO7A- 
               
               
                   
                 SEQ ID  
                 CTlong-v3.HA (SEQ ID NO: 51) 
               
               
                   
                 NO: 57 (nt) 
                   
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-noDIM-NTlong  
               
               
                   
                 NO: 88 (aa) 
                 (SEQ ID NO: 37) + AAV- 
               
               
                   
                 SEQ ID  
                 hMYO7A-CTlong-v2.HA  
               
               
                   
                 NO: 58 (nt) 
                 (SEQ ID NO: 38) 
               
               
                   
                   
                 AAV-smCBA-hMYO7A-noDimNT-CMv1  
               
               
                   
                   
                 (SEQ ID NO: 36) + AAV- 
               
               
                   
                   
                 hMYO7A-CTlong-v2.HA  
               
               
                   
                   
                 (SEQ ID NO: 38) 
               
               
                   
                   
               
               
                   
                 SEQ ID  
                 AAV-smCBA-hMYO7A-noDIMNTlong  
               
               
                   
                 NO: 89 (aa) 
                 (SEQ ID NO: 37) + (AAV- 
               
               
                   
                 SEQ ID  
                 hMYO7A-CTlong-v3.HA  
               
               
                   
                 NO: 59 (nt) 
                 (SEQ ID NO: 51) 
               
               
                   
                   
                 AAV-smCBA-hMYO7A-noDIMNTlong- 
               
               
                   
                   
                 CMv1 (SEQ ID NO: 36) + (AAV- 
               
               
                   
                   
                 hMYO7A-CTlong-v3.HA  
               
               
                   
                   
                 (SEQ ID NO: 51) 
               
               
                   
                   
               
            
           
           
               
            
               
                 smCBA Promoter 
               
            
           
           
               
               
               
            
               
                   
                 SEQ ID  
                 (SEQ ID NO: 1) 
               
               
                   
                 NO: 64 (nt) 
                 AAV-smCBA-hMYO7A-NT-Ex21- 
               
               
                   
                   
                 APSD-APhead (SEQ ID NO: 31) 
               
               
                   
                   
                 (SEQ ID NO: 46) 
               
               
                   
                   
                 (SEQ ID NO: 34) 
               
               
                   
                   
                 (SEQ ID NO: 3) 
               
               
                   
                   
                 (SEQ ID NO: 33) 
               
               
                   
                   
                 (SEQ ID NO: 37) 
               
               
                   
                   
                 (SEQ ID NO: 36) 
               
               
                   
                   
                 (SEQ ID NO: 50) 
               
               
                   
                   
               
            
           
           
               
            
               
                 HA tag 
               
            
           
           
               
               
               
            
               
                   
                 SEQ ID  
                 AAV-APhead-APSA-ex22hMYO7A-CT.HA  
               
               
                   
                 NO: 72 (nt) 
                 (SEQ ID NO: 32) 
               
               
                   
                   
                 AAV-APhead-APSA-ex22hMYO7A-CT.HA- 
               
               
                   
                   
                 CMv2 (SEQ ID NO: 35) 
               
               
                   
                   
                 AAV-hMYO7A-CTlong-v2.HA  
               
               
                   
                   
                 (SEQ ID NO: 38) 
               
               
                   
                   
                 AAV-APhead-APSA-hMYO7ACTex22- 
               
               
                   
                   
                 CMv2.1 (SEQ ID NO: 44) 
               
               
                   
                   
                 AAV-APhead-APSA-hMYO7ACT  
               
               
                   
                   
                 ex22-CMv2.1.HA (SEQ ID NO: 47) 
               
               
                   
                   
                 AAV-APhead-APSA-hMYO7ACTex  
               
               
                   
                   
                 22.HA-MIN (SEQ ID NO: 48) 
               
               
                   
                   
               
            
           
         
       
     
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     EQUIVALENTS 
     It should be understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and the scope of the appended claims. 
     All references cited herein (including publications, patent applications and patents) are incorporated by reference to the same extent as if each reference was individually and specifically incorporated by reference, and was set forth in its entirety herein. 
     Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. 
     All methods described herein can be performed in any suitable order, unless otherwise indicated herein, or unless otherwise clearly contradicted by context. 
     The use of any examples, or exemplary language (e.g., “such as”) provided herein, is intended merely to better illustrate the disclosure and does not pose a limitation on the scope of the disclosure unless otherwise indicated. No language in the specification should be construed as indicating any element is essential to the practice of the disclosure unless as much is explicitly stated. 
     The description herein of any aspect or embodiment of the disclosure using terms such as “comprising”, “having”, “including” or “containing” with reference to an element or elements is intended to provide support for a similar aspect or embodiment of the disclosure that “consists of”, “consists essentially of”, or “substantially comprises” that particular element or elements, unless otherwise stated or clearly contradicted by context (e.g., a composition described herein as comprising a particular element should be understood as also describing a composition consisting of that element, unless otherwise stated or clearly contradicted by context). For example, a nucleotide sequence described herein as comprising a particular element should be understood as also describing a nucleotide sequence consisting of that element, unless otherwise stated or clearly contradicted by context. 
     All of the compositions and methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. While the compositions and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations may be applied to the compositions and/or methods disclosed herein, and/or to the steps or the sequence of steps of the methods described herein without departing from the concept, spirit and/or scope of the disclosure. More specifically, it will be apparent that certain agents that are chemically- and/or physiologically-related may be substituted for the agents described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the disclosure as defined by the appended claims.