Patent Publication Number: US-6037122-A

Title: Nucleotide fragment of the 16S ribosomal RNA of corynebacteria, derived probes and primers, reagent and method of detection

Description:
The present belongs to the field of the techniques of detection and/or amplification of nucleic acid material using oligonucleotide probes or primers, and to their application, in particular, in testing for the presence or in the identification of bacteria of the genus Corynebacterium. 
     BACKGROUND OF THE INVENTION 
     Corynebacteria are commensal bacteria constituting a group which is heterogeneous from the morphological, taxonomic, pathogenic and therapeutic standpoints. This heterogeneity was, for a long time, responsible for a lack of knowledge of this group and limited the identification of the different strains. In man, the pathogenicity of the species belonging to the genus Corynebacterium is very variable, and many corynebacteria form part of the normal flora of the skin and the mucosae. Thus, from a pathological standpoint, some strains are highly pathogenic for man (Corynebacterium diphtheriae), but most are opportunistic bacteria responsible for infections in immunosuppressed or weakened patients. In effect, from an epidemiological point of view, the pathogenic power of corynebacteria was for a long time limited to diphtheria (Corynebacterium diphtheriae), but this disease is currently on the decline on account, in particular, of obligatory vaccination measures, and has given way to new pathologies associated with the advances in hospital medicine. Thus, corynebacteria are more and more frequently isolated in the context of nosocomial infections. 
     Since these infections caused by corynebacteria (see table below) occur essentially in greatly weakened patients, it is important to have at one&#39;s disposal a bacteriological diagnostic test which is sufficiently specific and sensitive to permit a rapid and selective detection of the species responsible, so as to implement a suitable therapy as quickly as possible. Now, at the resent time, analytical laboratories have at their disposal only biochemical and bacteriological tests limited by steps of isolation and of bacterial culture which are essential to the tests, and which are often not sufficiently specific and sensitive to diagnose the presence of a particular species of corynebacteria, and more especially of a pathogenic species, in a biological sample. 
     SUMMARY OF THE INVENTION 
     The present invention remedies the drawbacks mentioned above for revealing the presence of bacteria of the genus Corynebacterium, and more especially of the pathogenic species, by the use of a genetic marker in a method of detection by hybridization of nucleic acids combining specificity, sensitivity and speed. 
     
                                           TABLE                                   
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                                      wound infections                    
                                                       urinary  (suppurati
                                                    on,                   
  endocarditis septicemia infections pneumonia abscesses) pharyngitis     
                                                    adenophathy           
__________________________________________________________________________
Corynebacterium jelkeium                                                  
                +++   ++              +                                   
  Corynebacterium minutissimum  +                                         
  Corynebacterium pseudotuberculosis    +   +                             
  Corynebacterium ulcerans      +                                         
  Corynebacterium xerosis + +   +                                         
  Corynebacterium du groupe A4  +                                         
  Corynebacterium du groupe D2   +++  +                                   
  Corynebacterium du groupe G2 + +                                        
  Corynebacterium du groupe I     +                                       
  Arcanobacterium haemolyticum     + +                                    
  Rhodococcus equl    +                                                   
__________________________________________________________________________
 
    
     Before the invention is described, different terms used in the description and the claims will now be defined: 
     pathogenic species belonging to the genus Corynebacterium is understood to mean the species capable of causing, in man or in animals, an infection or a secondary infection, as opposed to the species of the same genus which make up the normal flora of the skin and the mucosae; any species which does not induce a pathological state in a patient such as a healthy patient but causes lesions in another patient such as an immunosuppressed patient is also considered to be a pathogenic species; in particular, some species which are non- pathogenic in healthy subjects are encountered in the hospital environment where immunosuppressed patients, in whom these same species are pathogenic, are staying; 
     &#34;nucleic acid extracted from bacteria&#34; is understood to mean either the total nucleic acid, or the ribosomal RNA, especially the 16S rRNA, or the genomic DNA, or alternatively the DNA obtained from the reverse transcription of the ribosomal RNA, especially the 16S RNA; 
     according to the invention, a nucleotide fragment or an oligonucleotide is a linked arrangement of monomers, characterized by the informational sequence of the natural nucleic acids, capable of hybridizing with a nucleotide fragment under predetermined conditions, it being possible for the linked arrangement to contain monomers of different structures and to be obtained from a natural nucleic acid molecule and/or by genetic recombination and/or by chemical synthesis; 
     thus, a monomer can be a natural nucleotide of nucleic acid whose constituent components are sugar, a phosphate group and a nitrogenous base; in RNA the sugar is ribose, in DNA the sugar is 2-deoxyribose; depending on whether the nucleic acid is DNA or RNA, the nitrogenous base is chosen from adenine, guanine, uracil, cytosine and thymine; or a nucleotide modified in at least one of the three constituent components; as an example, the modification can occur in the bases, generating modified bases such as inosine, 5-methyldeoxycytidine, deoxyuridine, 5-(dimethylamino)deoxyuridine, 2,6-diaminopurine, 5-bromodeoxyuridine and any other modified base favoring hybridization, in the sugar, namely the replacement of at least one deoxyribose by a polyamide (P. E. Nielsen et al., Science, 254, 1497-1500 (1991)), or in the phosphate group, for example its replacement by esters chosen, in particular, from diphosphate, alkyl- and arylphosphonate and phosphorothioate esters; 
     &#34;informational sequence&#34;, is understood to mean any ordered succession of monomers whose chemical nature and order in a reference direction constitute an item of information of the same quality as that of the natural nucleic acids; 
     &#34;hybridization&#34; is understood to mean the process during which, under suitable conditions, two nucleotide fragments having sufficiently complementary sequences are capable of associating via stable and specific hydrogen bonds to form a double strand. The hybridization conditions are determined by the &#34;stringency&#34;, that is to say the severity of the working conditions; hybridization is all the more specific for being performed at higher stringency; the stringency is a function, in particular, of the base composition of the probe/target duplex, as well as of the degree of mismatch between two nucleic acids; the stringency may also be a function of the parameters of the hybridization reaction, such as the concentration and type of ionic species present in the hybridization solution, the nature and concentration of denaturating agents and/or the hybridization temperature; the stringency of the conditions under which a hybridization should be carried out depends, in particular, on the probes used; all these data are well known to a person skilled in the art, and the appropriate conditions may be determined, where appropriate, in each case by routine experiments; in general, depending on the length of the probes used, the temperature for the hybridization reaction is between approximately 20 and 65° C., and especially between 35 and 65° C., in a saline solution at a concentration of approximately 0.8 to 1M; 
     a probe is a nucleotide fragment comprising at least 5 monomers, and advantageously at least 8 monomers, possessing a specificity of hybridization under particular conditions to form a hybridization complex with a nucleotide fragment having a particular nucleotide sequence included in the ribosomal RNA, or the DNA obtained by reverse transcription of said ribosomal RNA, or the DNA of which said ribosomal RNA is the transcription product; a probe may be used for diagnostic purposes and be, in this case, a capture and/or detection probe, or for therapeutic purposes; 
     the capture probe may be immobilized on a solid support by any suitable means, that is to say directly or indirectly, for example by covalent bonding or passive adsorption; 
     the detection probe is labeled by means of a label chosen from radioactive isotopes, enzymes chosen, in particular, from peroxidase and alkaline phosphatase and those capable of hydrolyzing a chromogenic, fluorogenic or luminescent substrate, chromophoric chemical compounds, chromogenic, fluorogenic or luminescent compounds, nucleotide base analogs and biotin; 
     the probes used for diagnostic purposes of the invention may be employed in all known hybridization techniques, and in particular the techniques termed &#34;dot-blot&#34; (Maniatis et al., Molecular Cloning, Cold Spring Harbor, 1982), &#34;Southern blot&#34; (Southern E. M., J. Mol. Biol., 98, 503 (1975)), &#34;northern blot&#34;, which is a technique identical to the &#34;Southern blot&#34; technique but which uses RNA as target, and the sandwich technique (Dunn A. R., Hassel J. A., Cell, 12, 23 (1977)); advantageously, the sandwich technique is used in the present invention, comprising a specific capture probe and/or a specific detection probe, on the understanding that the capture probe and the detection probe must possess an at least partially different nucleotide sequence; 
     another application of the invention is a therapeutic probe for treating infections due to corynebacteria, said probe being capable of hybridizing in vivo with the 16S ribosomal RNA of said bacteria and/or with the genomic DNA, to block the phenomena of translation and/or transcription; 
     a primer is a probe comprising from 5 to 30 monomers, possessing a specificity of hybridization under particular conditions for the initiation of an enzymatic polymerization, for example in an amplification technique such as PCR (polymerase chain reaction), in an elongation process such as sequencing, in a method of reverse transcription or the like; 
     homology characterizes the degree of identity of two nucleotide fragments compared: it is measured by the percentage identity which is determined, in particular, by direct comparison of nucleotide sequences with respect to reference nucleotide sequences; 
     any nucleotide fragment is termed equivalent to or derived from a reference fragment if it possesses a nucleotide sequence equivalent to the reference sequence, such as: 
     a) any fragment capable of hybridizing at least partially with the complement of the reference fragment, 
     b) any fragment whose alignment with the reference fragment leads to the revelation of a larger number of identical contiguous bases than with any other fragment originating from another taxonomic group, 
     c) any fragment resulting or capable of resulting from the natural variability of the species from which it is obtained, 
     d) any fragment capable of resulting from genetic engineering techniques applied to the reference fragment, 
     e) any fragment differing from the reference fragment by insertion, deletion or substitution of at least one monomer, or extension or shortening at one of its ends at least. 
     In particular, the probes and primers according to the present invention are those whose sequences are identical or equivalent to one of the sequences identified below, in particular the sequences possessing at least 70% homology with one of said sequences and a succession of at least 5 contiguous monomers belonging to this sequence. 
     According to the invention, single-stranded nucleotide fragments of the 16S ribosomal RNA belonging to specific regions of at least one species of corynebacteria have been determined. For 21 pathogenic species of corynebacteria, these fragments are shown in the sequence listing given at the end of the description, and identified by their number SEQ ID NO 1 to 91. These 21 pathogenic species are the following: 
     Corynebacterium afermentans afermantans 
     Corynebacterium afermantans lipophilum 
     Corynebacterium amycolatum 
     Corynebacterium argentoratense 
     Corynebacterium asperum 
     Corynebacterium bovis 
     Corynebacterium cystitidis 
     Corynebacterium diphtheriae 
     Corynebacterium flavescens 
     Corynebacterium jeikeium 
     Corynebacterium matruchotii 
     Corynebacterium minutissimum 
     Corynebacterium mycetoides 
     Corynebacterium pilosum 
     Corynebacterium pseudodiphteriticum 
     Corynebacterium pseudotuberculosis 
     Corynebacterium seminale 
     Corynebacterium sp group F-1 
     Corynebacterium striatum 
     Corynebacterium ulcerans 
     Corynebacterium urealyticum 
     The fragments of the invention are defined by a numbering of the nucleotides using as reference the numbering of the nucleotide sequence of the 16S ribosomal RNA of Corynebacterium diphtheriae, which has been completely identified by the Applicant and is described at the end of the description under the reference SEQ ID NO 92. 
     The single-stranded nucleotide fragments of the invention possess a nucleotide sequence chosen from the following sequences (numbering with reference to SEQ ID NO 92): 
     beginning at nucleotide No. 39 and ending at nucleotide No. 67 
     beginning at nucleotide No. 162 and ending at nucleotide No. 182 
     beginning at nucleotide No. 433 and ending at nucleotide No. 461 
     beginning at nucleotide No. 575 and ending at nucleotide No. 598 
     beginning at nucleotide No. 805 and ending at nucleotide No. 820 
     beginning at nucleotide No. 826 and ending at nucleotide No. 842 
     beginning at nucleotide No. 980 and ending at nucleotide No. 1000 of the 16S subunit of the ribosomal RNA of any pathogenic species belonging to the genus Corynebacterium, according to the sequencing of said subunit of Corynebacterium diphtheriae, and their complementary sequences. 
     More especially, the nucleotide sequences of the fragments of the invention are chosen from the sequences SEQ ID NO 1 to SEQ ID NO 91 described at the end of the description, and their complementary sequences. 
     According to the above definition, the fragments of the invention are RNA fragments, but they can also be single-stranded DNA fragments resulting from the reverse transcription of the abovementioned RNA fragments, or their complementary fragments, as well as single-stranded fragments of genomic DNA whose transcription products are the abovementioned RNA fragments, or their complementary fragments. 
     Another subject according to the present invention is nucleic acid probes or primers specific for pathogenic species of corynebacteria. 
     Preferred probes are those possessing a sequence included in the sequences (numbering with reference to SEQ ID NO 92): 
     beginning at nucleotide No. 39 and ending at nucleotide No. 67 
     beginning at nucleotide No. 162 and ending at nucleotide No. 182 
     beginning at nucleotide No. 433 and ending at nucleotide No. 461 
     beginning at nucleotide No. 575 and ending at nucleotide No. 598 
     beginning at nucleotide No. 805 and ending at nucleotide No. 820 
     beginning at nucleotide No. 826 and ending at nucleotide No. 842 
     beginning at nucleotide No. 980 and ending at nucleotide No. 1000, and their complementary sequences, and more especially a sequence included in the identified sequences SEQ ID NO 1 to SEQ ID NO 91 and their complementary sequences. 
     Another subject of the invention is a primer for the specific reverse transcription of the 16S ribosomal RNA sequence of corynebacteria into a complementary DNA sequence, or a primer, in particular, for the specific amplification by a polymerization chain reaction of the DNA sequence complementary to a 16S ribosomal RNA sequence of pathogenic corynebacteria. 
     The value of the probes and primers of the invention was, in addition, demonstrated when it was observed that they are specific for the pathogenic species of corynebacteria compared with species belonging to a different genus of Corynebacterium but phylogenetically close to corynebacteria. 
     The invention also relates to a reagent for detecting and/or identifying at least one pathogenic species of corynebacteria in a biological sample, comprising at least one probe of the invention, and especially a capture probe and a detection probe, either or both of these corresponding to the definition of a probe according to the invention. The reagent can comprise a mixture of probes of the invention with the object of detecting at least two pathogenic species of corynebacteria. 
     Lastly, the invention provides a method for detecting selectively and/or identifying a pathogenic species of corynebacteria in a biological sample, according to which the 16S ribosomal RNA extracted from the bacteria present in the sample, and denatured where appropriate, or the extracted and denatured genomic DNA of the bacteria, or the DNA obtained from the reverse transcription of the 16S ribosomal RNA, is exposed to at least one probe of the invention, and the hybridization of said probe is detected. 
     Preferably, before the DNA is exposed to the probe of the invention, an amplification of this DNA is carried out in the presence of an appropriate enzyme system and at least one amplification primer of the invention, and optionally a eubacterial primer. 
    
    
     The subjects of the invention and some of their applications are described below in Examples 1 and 2. 
     EXAMPLE 1 
     Revelation of the specificity of the probes and primers of the invention with respect to 22 phylogenetically close species of corynebacteria. 
     For 22 species not belonging to the genus Corynebacterium, fragments of the 16S ribosomal RNA corresponding to the fragments of the invention were determined. 
     The species are the following: D. maris, D. sp @1, D. sp. @2, D. sp @3, G. aichiensis, G. bronchialis @1, G. sputi @1, G. terrae @1, M. tuberculosis @2, N. asteroides @1, N. brasiliensis, N. carnea, N. nova, N. seriolae, N. sp @1, R. equi @1, R. erythropolis @1, R. fascians @1, R. globerulus @1, R. luteus, R. rhodochrous @1, T. paurometabolum @1. 
     These fragments were determined from the complete sequencing of the 16S ribosomal RNA of these species. 
     The total nucleic acid of the strains used was isolated by the method of Sjo bring et al. (1990. Polymerase chain reaction for detection of Mycobacterium tuberculosis. J. Clin. Microbiol. 28(10):2200-2204). PCR amplification products which cover 90% of the sequence were generated from the ribosomal RNA using amplification primers of eubacterial specificity. 
     The amplification products obtained were sequenced directly by the chain termination method (Sanger et al., Proc. Natl. Acad. Sci. U.S.A., 1977, 74: 5463-5467), by thermal cyclization using a heat-stable Taq DNA polymerase (Perkin). 
     An alignment of the fragments obtained with the fragments thus determined reveals the specific character of the fragments of the invention. 
     EXAMPLE 2 
     Use of a specific probe directed against the 16S ribosomal RNA for the identification of Corynebacterium striatum. 
     The specificity of the probe corresponding to the complementary strand of the fragment beginning at nucleotide No. 6 and ending at nucleotide No. 22 of SEQ NO 18 was tested for the strains of Corynebacterium striatum. 
     A collection of strains of corynebacteria was tested by hybridization with the 16S ribosomal RNA and enabled the specificity of this probe to be established a posteriori. 
     The hybridization of the ribosomal RNAs of a target bacterium was conducted according to the nonradioactive and semi-automated detection method described in French Patent No 90/07249. A capture probe S8L corresponding to a probe of eubacterial specificity (described in Patent Application FR No. 93/02127) and an oligonucleotide (corresponding to the probe defined at the beginning of Example 2) -enzyme specific detection conjugate are used. The manipulation was conducted in a microtitration plate according to the following protocol. 
     The ribosomal RNA of the strains was extracted according to the basic protocol for extraction of the RNA of Gram-positive bacteria described in &#34;Current Protocols in Molecular Biology&#34; 1987, Ausubel FM et al., Wiley interscience, New York. A solution of 1 ng/μl of the capture oligonucleotide, the 5&#39; end of which has reacted with the reagent Aminolink 2 (an amine having the following structure: ##STR1## (Applied Biosystems, Foster City, Calif.), in PBX 3 (0.45 M NaCl, 0.15 M sodium phosphate, pH 7.0) is introduced into a microtitration plate (Nunc 439454). The plate is incubated for 2 h at 37° C. and then washed 3 times with 300 μl of PBST (PBS 1, Tween 20: 0.05% (Merck 822184)). The reagent Aminolink 2 enables an arm containing a 6-aminohexyl group to be added at the 5&#39; end of the probe. The probe coupled in this way to a ligand possessing a polar (primary amine) group, and bound passively to the support, elicits an improved signal; see Application FR 91/09057. 
     The target consisting of 10 μl of extract of total RNAs is mixed with 40 μl of PBS-salmon buffer (PBS 3+10 μg/ml salmon sperm DNA (Sigma D 9156)). The mixture is added into the well in which the capture probe is bound, with 50 μl of a solution of the oligonucleotide-peroxidase conjugate, constituting the detection probe, at an oligonucleotide concentration of 0.1 ng/μl in a PBS-horse buffer (PBS 3+10% horse serum (BioMe rieux 55842)). The plate is incubated for 1 h at 37° C. and then washed 3 times with 300 μl of PBST buffer. Into each well are added 100 μl of OPD (ortho-phenylenediamine, Cambridge Medical Biotechnology ref/456) substrate in a 0.055 M citric acid, 0.1 M sodium monohydrogen phosphate buffer, pH 4.93, at a concentration of 4 mg/ml, to which 30% hydrogen peroxide diluted to 1/1000 is added at the time of use. After 20 min of reaction, the enzyme activity is blocked with 100 μl of 1N sulfuric acid and reading is performed on an Axia Microreader (AXIA: registered trademark) apparatus (bioMe rieux) at 492 nm. 
     The target bacteria were, in particular, the following: 
     8 isolates of Corynebacterium striatum 
     1 strain of the following species: 
     Corynebacterium afermentans afermantans 
     Corynebacterium afermantans lipophilum 
     Corynebacterium amoniagenes (nonpathogenic) 
     Corynebacterium amycolatum 
     Corynebacterium argentoratense 
     Corynebacterium asperum 
     Corynebacterium bovis 
     Corynebacterium callunae (nonpathogenic) 
     Corynebacterium cystitidis 
     Corynebacterium diphtheriae 
     Corynebacterium flavescens 
     Corynebacterium glutamicum (nonpathogenic) 
     Corynebacterium jeikeium 
     Corynebacterium matruchotii 
     Corynebacterium minutissimum 
     Corynebacterium mycetoides 
     Corynebacterium pilosum 
     Corynebacterium propinquum (nonpathogenic) 
     Corynebacterium pseudodiphteriticum 
     Corynebacterium pseudotuberculosis 
     Corynebacterium seminale 
     Corynebacterium sp group F-1 
     Corynebacterium ulcerans 
     Corynebacterium urealyticum 
     other bacterial species: 
     M. tuberculosis, N. asteroi des, R. equi 
     The results obtained indicate that the combination of probes used is, a posteriori, specific for Corynebacterium striatum. It does not display a cross reaction with the nucleic acids, especially the ribosomal RNA, of the other bacterial species. It was checked that the target ribosomal RNAs of the other species are indeed released during the step of lysis, since they react with a combination of capture and detection probes directed against 16S ribosomal RNA and of eubacterial specificity. 
     Adaptation of the specific combination to the VIDAS (registered trademark--marketed by the company bioMe rieux-VITEK) automated system was performed. The wall of the microplate well is replaced here by the SPR (trademark) (&#34;Solid Phase Receptacle&#34;), which is a conical support made from a material sold under the name K resin (butadiene-styrene copolymer) and marketed by the company bioMe rieux-VITEK (U.S.A.). The various reagents are arranged in a multicuvette carrier bar, and the different steps proceed in the SPR which is capable of drawing in and expelling the reagents and which hence acts as a pipette. The sandwich hybridization reaction described in the protocol below takes place on the inner wall of the cone. 
     The capture oligonucleotide containing the ligand Aminolink 2 (Applied Biosystems ref. 400808) at its 5&#39; end, at a concentration of 1 ng/μl in a volume of 315 μl of a PBS 4 solution (200 mM sodium phosphate, pH 7.0, 600 mM NaCl), is bound passively to the inner surface of the SPR. After one night at room temperature or two hours at 37° C., the cones are washed twice with a PBS-Tween solution and then dried under vacuum. The carrier bar contains in cuvettes the reagents needed for the detection, that is to say: 200 μl of a solution at a concentration of 0.1 ng/μl of the oligonucleotidealkaline phosphatase detection conjugate, twice 600 μl of PBS-Tween washing solution and a cuvette of substrate. 
     10 μl of the extracted RNA, in the same buffer as for the microplate protocol above, are introduced into the first well of the carrier bar. 
     After incubation of the cone for 30 minutes with the target plus detection probe mixture, the cone is washed twice with a PBS-Tween solution. 250 μl of MUP (4-methylumbelliferyl phosphate) substrate dissolved in a diethanolamine buffer are drawn into the cone and then released into a reading cuvette. The apparatus measures the fluorescence signal expressed in RFU (relative fluorescence units) of the cuvette. 
     The results obtained with this system are the same as those obtained in microplates. 
     
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           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #13:                    
  - - ACGGAAAGGC CUCUUCGGAG            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 14:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #14:                    
  - - ACGGAAAGGC CCCUUCGGGG            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 15:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 24 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #15:                    
  - - ACGGAAAGGC UCCAGCUUGC UGGG          - #                  - #        
        24                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 16:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 25 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #16:                    
  - - ACGGAAAGGC UCCUGCUUGC UAGGG          - #                  - #       
        25                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 17:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 24 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #17:                    
  - - ACGGAAAGGC AGUGCUUGCU ACUG          - #                  - #        
        24                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 18:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 25 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #18:                    
  - - ACGGAAAGGC CGCAGCUUGC UGCGG          - #                  - #       
        25                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 19:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 19 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #19:                    
  - - ACGGAAAGGC CCCUUCGGG             - #                  - #           
       - # 19                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 20:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 23 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #20:                    
  - - ACGGAAAGGC CCCUGCUUGC AGG           - #                  - #        
        23                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 21:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #21:                    
  - - CAUGCUUUAG UGUGUGUG             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 22:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #22:                    
  - - CACAUUUUGG AUGGUGUG             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 23:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #23:                    
  - - CAUGGUGUGG AUGCUGUG             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 24:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 19 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #24:                    
  - - CAUCCUUUAG UGUGUGAUG             - #                  - #           
       - # 19                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 25:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 17 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #25:                    
  - - CGCACCGUGA GGGUGUG             - #                  - #             
     - #   17                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 26:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #26:                    
  - - CGUGCUUUAG UGUGUGCG             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 27:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 17 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #27:                    
  - - GUAUCUUUUG UGGGUGU             - #                  - #             
     - #   17                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 28:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 17 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #28:                    
  - - GGUCUUUGGU GUGAUUG             - #                  - #             
     - #   17                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 29:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 19 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #29:                    
  - - CAUGGUUUAG UGUCUCAUG             - #                  - #           
       - # 19                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 30:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #30:                    
  - - CCGUCUUUAG UGUGGUGG             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 31:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #31:                    
  - - CUGCAUGUUG GUGUGUGG             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 32:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 7 base p - #airs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #32:                    
  - - CUUUGUG                 - #                  - #                  - 
#           7                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 33:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #33:                    
  - - CGCGCUUUAG UGUGUGUG             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 34:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #34:                    
  - - CGCAUCGUGG UUGGUGUG             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 35:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #35:                    
  - - AACUUUUUGG AUAUUGUU             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 36:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #36:                    
  - - AGCUGUUUAG UGUCAGUU             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 37:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #37:                    
  - - CAAUCUUUAG UGUGGUUG             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 38:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 17 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #38:                    
  - - GAAGCUUUUG UGACGGU             - #                  - #             
     - #   17                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 39:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 19 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #39:                    
  - - GAAGCGUAUU UGUGACGGU             - #                  - #           
       - # 19                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 40:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 17 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #40:                    
  - - GAAGGGUUUC UGACGGU             - #                  - #             
     - #   17                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 41:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 17 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #41:                    
  - - GAAGCGUGUG UGACGGU             - #                  - #             
     - #   17                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 42:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 23 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #42:                    
  - - GAAGCCUUUN NNAAGGUGAC GGU           - #                  - #        
        23                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 43:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 21 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #43:                    
  - - GAAGCACUGU GUGGUGACGG U           - #                  - #          
        - #21                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 44:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #44:                    
  - - GAAGCGUUUU GUGACGGU             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 45:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #45:                    
  - - GAAGCUUUUU GUGACGGU             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 46:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 18 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #46:                    
  - - GAAGCAUUAU GUGACGGU             - #                  - #            
      - #  18                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 47:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 17 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #47:                    
  - - GAAGCGUAAG UGACGGU             - #                  - #             
     - #   17                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 48:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 19 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #48:                    
  - - GAAGCCUUCG GGUGACGGU             - #                  - #           
       - # 19                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 49:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 24 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #49:                    
  - - CGUCUGUGAA AUUCCGGGGC UUAA          - #                  - #        
        24                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 50:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 24 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #50:                    
  - - CGUUUGUGUA AGCNNNCAGC UUAA          - #                  - #        
        24                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 51:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 24 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #51:                    
  - - CGUCUGUGAA AUCCCGGGGC UUAA          - #                  - #        
        24                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 52:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 24 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #52:                    
  - - CGUCUGUGGA AGUCCGGGGC UUAA          - #                  - #        
        24                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 53:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 24 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #53:                    
  - - CGUUUGUGUA AGUCCACGGC UUAA          - #                  - #        
        24                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 54:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 24 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #54:                    
  - - CGUCUGUGAA AUUCCACAGC UUAA          - #                  - #        
        24                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 55:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 24 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #55:                    
  - - CGUCUGUGUA AUCCAGGGGC UUAA          - #                  - #        
        24                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 56:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 24 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #56:                    
  - - CGUUUGUGUA AGUCCACAGC UUAA          - #                  - #        
        24                                                                
  - -  - - (2) INFORMATION FOR SEQ ID NO: 57:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 15 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #57:                    
  - - UAGGUGUGAG GGUCU              - #                  - #              
    - #    15                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 58:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 15 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #58:                    
  - - UAGGUGUGAG UCCCU              - #                  - #              
    - #    15                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 59:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 15 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #59:                    
  - - UAGGUGUAGG GGGCU              - #                  - #              
    - #    15                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 60:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #60:                    
  - - UAGGUGUGGG UUUCCU             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 61:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 15 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #61:                    
  - - UAGGUGUAGG GGACU              - #                  - #              
    - #    15                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 62:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 15 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #62:                    
  - - UAGGUGUGGG GAUCU              - #                  - #              
    - #    15                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 63:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 15 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #63:                    
  - - UAGGUGUGAG AUCCU              - #                  - #              
    - #    15                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 64:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #64:                    
  - - UAGGUGUGGG GGUUUN             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 65:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 14 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #65:                    
  - - UAGGUGUGGG GACC              - #                  - #               
   - #     14                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 66:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 15 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #66:                    
  - - UAGGUGUAGG GACCU              - #                  - #              
    - #    15                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 67:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #67:                    
  - - UAGGUGUGAG UCCCNU             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 68:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 15 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #68:                    
  - - UAGGUGUGGG GAUUU              - #                  - #              
    - #    15                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 69:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #69:                    
  - - GACUUUCGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 70:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #70:                    
  - - GGGGUUCGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 71:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #71:                    
  - - GUCUUCUGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 72:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #72:                    
  - - GAUUUCCGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 73:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #73:                    
  - - GGGUUUUGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 74:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #74:                    
  - - GAUUCCCGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 75:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #75:                    
  - - GGUUUCNGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 76:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #76:                    
  - - GUUUUCUGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 77:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #77:                    
  - - GGUUUCUGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 78:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #78:                    
  - - GGGGUUUGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 79:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 16 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #79:                    
  - - GGGAUUUGUG CCGUAG             - #                  - #              
    - #    16                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 80:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #80:                    
  - - UGCAGGAUCG GCGUAGUGAU            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 81:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #81:                    
  - - CACCAGAUCG CCGUAGAGAU            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 82:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #82:                    
  - - UACAGGAUCG CGCCAGAGAU            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 83:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #83:                    
  - - UACAGGAUCG CUGCAGAGAU            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 84:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #84:                    
  - - GGCAGGACCG GCGUGGAGAC            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 85:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #85:                    
  - - CACUGGAUUG CCAUGGAGAC            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 86:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #86:                    
  - - UGCAGGAUUG GGUCAGAGAU            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 87:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 21 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #87:                    
  - - CACCAGACGG UCGUAGAGAU A           - #                  - #          
        - #21                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 88:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #88:                    
  - - CACUAGAUCG CUGUAGAGAU            - #                  - #           
      20                                                                  
  - -  - - (2) INFORMATION FOR SEQ ID NO: 89:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #89:                    
  - - CACCGGAUCG GGCUAGAGAU            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 90:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #90:                    
  - - UACGGGAUCG CCGCAGAGAU            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 91:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 20 base - #pairs                                   
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #91:                    
  - - UACAAGACAG GCGUAGAGAU            - #                  - #           
       - # 20                                                             
  - -  - - (2) INFORMATION FOR SEQ ID NO: 92:                             
  - -      (i) SEQUENCE CHARACTERISTICS:                                  
           (A) LENGTH: 1475 base - #pairs                                 
           (B) TYPE: nucleotide                                           
           (C) STRANDEDNESS: single                                       
           (D) TOPOLOGY: linear                                           
  - -     (ii) MOLECULE TYPE: rRNA                                        
  - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - #92:                    
  - - CGCUGGCGGC GUGCUUAACA CAUGCAAGUC GAACGGAAAG GCCUAGCUUG CU -         
#AGGUACUC     60                                                          
   - - GAGUGGCGAA CGGGUGAGUA ACACGUGGGU GAUCUGCCUC GUACUUCGGG AU -        
#AAGCCUGG    120                                                          
   - - GAAACUGGGU CUAAUACUGG AUAGGACCAU GCUUUAGUGU GUGUGGUGGA AA -        
#GUUUUUCG    180                                                          
   - - GUACGAGAUG AGCCCGCGGC CUAUCAGCUU GUUGGUGGGG UAAUGGCCUA CC -        
#AAGGCGUC    240                                                          
   - - GACGGGUAGC CGGCCUGAGA GGGUGGACGG CCACAUUGGG ACUGAGAUAC GG -        
#CCCAGACU    300                                                          
   - - CCUACGGGAG GCAGCAGUGG GGAAUAUUGC ACAAUGGGCG CAAGCCUGAU GC -        
#AGCGACGC    360                                                          
   - - CGCGUGGGGG AUGACGGCCU UCGGGUUGUA AACCUCUUUC GCUAGGGACG AA -        
#GCUUUUGU    420                                                          
   - - GACGGUACCU AGAUAAGAAG CACCGGCUAA CUACGUGCCA GCAGCCGCGG UA -        
#AUACGUAG    480                                                          
   - - GGUGCGAGCG UUGUCCGGAA UUACUGGGCG UAAAGAGCUC GUAGGUGGUU UG -        
#UCGCGUCG    540                                                          
   - - UCUGUGAAAU UCCGGGGCUU AACUUCGGGC GUGCAGGCGA UACGGGCAUA AC -        
#UUGAGUGC    600                                                          
   - - UGUAGGGGAG ACUGGAAUUC CUGGUGUAGC GGUGGAAUGC GCAGAUAUCA GG -        
#AGGAACAC    660                                                          
   - - CGAUGGCGAA GGCAGGUCUC UGGGCAGUAA CUGACGCUGA GGAGCGAAAG CA -        
#UGGGGAGC    720                                                          
   - - GAACAGGAUU AGAUACCCUG GUAGUCCAUG CCGUAAACGG UGGGCGCUAG GU -        
#GUGAGGGU    780                                                          
   - - CUUCCACGAC UUUCGUGCCG UAGCUAACGC AUUAAGCGCC CCGCCUGGGG AG -        
#UACGGCCG    840                                                          
   - - CAAGGCUAAA ACUCAAAGGA AUUGACGGGG GCCCGCACAA GCGGCGGAGC AU -        
#GUGGAUUA    900                                                          
   - - AUUCGAUGCA ACGCGAAGAA CCUUACCUGG GCUUGACAUA UGCAGGAUCG GC -        
#GUAGUGAU    960                                                          
   - - ACGUUUUCCC UUGUGGUCUG UAUACAGGUG GUGCAUGGUU GUCGUCAGCU CG -        
#UGUCGUGA   1020                                                          
   - - GAUGUUGGGU UAAGUCCCGC AACGAGCGCA ACCCUUGUCU UAUGUUGCCA GC -        
#ACGUGAUG   1080                                                          
   - - GUGGGGACUC AUGAGAGACU GCCGGGGUUA ACUCGGAGGA AGGUGGGGAU GA -        
#CGUCAAAU   1140                                                          
   - - CAUCAUGCCC CUUAUGUCCA GGGCUUCACA CAUGCUACAA UGGUCGGUAC AA -        
#CGCGCUGC   1200                                                          
   - - GAGCCUGUGA GGGUGAGCGA AUCGCUGAAA GCCGGCCUCA GUUCGGAUUG GG -        
#GUCUGCAA   1260                                                          
   - - CUCGACCCCA UGAAGUCGGA GUCGCUAGUA AUCGCAGAUC AGCAACGCUG CG -        
#GUGAAUAC   1320                                                          
   - - GUNCCCGGGC CUUGUACACA CCGCCCGUCA CGUCAUGAAA GUUGGUAACA CC -        
#CGAAGCCA   1380                                                          
   - - GUGGCCUAAC CCUUGUGGGG GGGAGCUGUC GAAGGUGGNA UCGGCGAUUG GN -        
#ACGAAGUC   1440                                                          
   - - GUAACAAGGU AGCCGUACCG GAAGGUGCGG GCUGG       - #                   
- #     1475                                                              
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