Patent Publication Number: US-2022229051-A1

Title: Methods and compositions for polymer superparamagnetic particles for nucleic acid extraction

Description:
CROSS-REFERENCE TO RELATED APPLICATION 
     This application claims priority to U.S. Provisional Application Ser. No. 62/577,680 filed Oct. 26, 2017 and fully incorporated herein by reference for all purposes. 
    
    
     BACKGROUND 
     Magnetic particles have received a great deal of attention in biological, medical, diagnostic and engineering areas. Unfortunately, manufacturing cost and complexity associated with use of such particles have been barriers to broader implementation. 
     INCORPORATION BY REFERENCE 
     All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference. 
     COPYRIGHT 
     This document contains material subject to copyright protection. The copyright owner (Applicant herein) has no objection to facsimile reproduction of the patent documents and disclosures, as they appear in the US Patent and Trademark Office patent file or records, but otherwise reserves all copyright rights whatsoever. The following notice shall apply: Copyright 2017 Theranos, Inc. 
     SUMMARY 
     The disadvantages associated with the prior art are overcome by embodiments described herein. 
     Magnetic nanoparticles or microparticles as disclosed herein are suitable for biological, medical, diagnostic and engineering areas, because of their magnetic properties, versatility in immunity separations, applications as Mill contrast agent, biosensor and the targeted drug delivery. Of particular interest are superparamagnetic particles constructed from materials such as but not limited to Iron (II, III) oxide (Fe 3 O 4 -magnetite). 
     Superparamagnetic particles constructed from Iron (II, III) oxide (Fe 3 O 4 -magnetite) has potential in binding, extraction and purification of biomolecules including RNA, DNA, proteins, enzymes and organic small molecules; they may be used as MRI contrast agents; and as carriers of biomarkers and drugs. The versatility of superparamagnetic particles derives from the combination of an iron oxide core (typically Fe 3 O 4 ) with a variety of coating materials. When the particles are placed in a magnetic field, they develop a strong internal magnetization from exchange coupling of electrons. This allows their movement to be controlled by the external magnetic field. When the field is removed, particles are no longer magnetized and have no magnetic memory. However, Fe 3 O 4  is readily oxidized to hematite (Fe 2 O 3 ), changing its magnetism from superparamagnetic to ferromagnetic. To avoid oxidation and to protect the metal core, natural and synthetic polymers and silica have been employed to coat the magnetic particles. A wide range of chemical functional groups can be introduced on the coating surface to increase stability, wetting properties and binding flexibility for various applications. Chemical functionalization by amines, carboxylic acids, epoxy, and aldehydes is usually used to immobilize proteins, enzymes, RNA, DNA biomolecules on the surface via covalent linkages. 
     Other approaches have been developed because the use of ethanol and other solvents was not compatible with automated molecular diagnostic platforms. 
     In one embodiment described herein, a composition is provided comprising superparamagnetic particles. Optionally, the particles comprise microbeads. Optionally, the particles comprise nanobeads. Optionally, the particles are non-spherical particles. Optionally, the particles are non-spherical particles suitable for RNA or DNA extraction. 
     In another embodiment described herein, a method is provided for forming microparticles. Optionally, the method comprises using citrate precipitation. 
     In another embodiment described herein, a kit comprising one or more of the particles is provided. Optionally, a kit for sample preparation for nucleic acid extraction is provided comprising non-spherical microparticles or nanoparticles. 
     This Summary is provided to introduce a selection of concepts in a simplified form that are further described below in the Detailed Description. This Summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used to limit the scope of the claimed subject matter. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  shows one embodiment of a schematic of a Zika virus immunoassay. 
         FIG. 2  shows SEM picture of magnetic particles. 
         FIG. 3  shows specifications for magnetic particles. 
         FIG. 4A  shows images of magnetic beads. 
         FIGS. 4B-4D  show embodiments of synthesis techniques. 
         FIG. 5  shows a liter jacketed reactor. 
         FIG. 6  shows co-polymerization particles microscopic images. 
         FIG. 7  shows Erlenemeryer flasks for coating and amination steps. 
         FIG. 8  shows test results. 
         FIG. 9  shows color different Fe precipitation. 
         FIG. 10  shows aliquoted plans for the stockpile of beads intermediates. 
         FIG. 11  shows signal results. 
         FIGS. 12-15  shows data from various batches. 
         FIG. 16A  shows chemical structure of Biot-FAM. 
         FIG. 16B  shows a way of binding between Biot-FAM with Streptavidin. 
         FIGS. 16C-16J  shows data from various results. 
         FIG. 17  shows images of in-house PS seeds. 
         FIG. 18  shows images of in-house PS-PGMA coated seeds (left), co-polymerization formed PS-PGMA beads (right). 
         FIG. 19  shows in-house aminated beads. 
         FIG. 20  shows in-house Fe 3 O 4  beads 
         FIG. 21  shows one embodiment of the process of Streptavidin conjugated beads and its intermediates preparation. 
     
    
    
     DESCRIPTION OF THE SPECIFIC EMBODIMENTS 
     It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed. It may be noted that, as used in the specification and the appended claims, the singular forms “a”, “an” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a material” may include mixtures of materials, reference to “a compound” may include multiple compounds, and the like. References cited herein are hereby incorporated by reference in their entirety, except to the extent that they conflict with teachings explicitly set forth in this specification. 
     In this specification and in the claims which follow, reference will be made to a number of terms which shall be defined to have the following meanings: 
     “Optional” or “optionally” means that the subsequently described circumstance may or may not occur, so that the description includes instances where the circumstance occurs and instances where it does not. For example, if a device optionally contains a feature for a sample collection unit, this means that the sample collection unit may or may not be present, and, thus, the description includes both structures wherein a device possesses the sample collection unit and structures wherein sample collection unit is not present. 
     As used herein, the terms “substantial” means more than a minimal or insignificant amount; and “substantially” means more than a minimally or insignificantly. Thus, for example, the phrase “substantially different”, as used herein, denotes a sufficiently high degree of difference between two numeric values such that one of skill in the art would consider the difference between the two values to be of statistical significance within the context of the characteristic measured by said values. Thus, the difference between two values that are substantially different from each other is typically greater than about 10%, and may be greater than about 20%, preferably greater than about 30%, preferably greater than about 40%, preferably greater than about 50% as a function of the reference value or comparator value. 
     As used herein, a “sample” may be but is not limited to a blood sample, or a portion of a blood sample, may be of any suitable size or volume, and is preferably of small size or volume. In some embodiments of the assays and methods disclosed herein, measurements may be made using a small volume blood sample, or no more than a small volume portion of a blood sample, where a small volume comprises no more than about 5 mL; or comprises no more than about 3 mL; or comprises no more than about 2 mL; or comprises no more than about 1 mL; or comprises no more than about 500 μL; or comprises no more than about 250 μL; or comprises no more than about 100 μL; or comprises no more than about 75 μL; or comprises no more than about 50 μL; or comprises no more than about 35 μL; or comprises no more than about 25 μL; or comprises no more than about 20 μL; or comprises no more than about 15 μL; or comprises no more than about 10 μL; or comprises no more than about 8 μL; or comprises no more than about 6 μL; or comprises no more than about 5 μL; or comprises no more than about 4 μL; or comprises no more than about 3 μL; or comprises no more than about 2 μL; or comprises no more than about 1 μL; or comprises no more than about 0.8 μL; or comprises no more than about 0.5 μL; or comprises no more than about 0.3 μL; or comprises no more than about 0.2 μL; or comprises no more than about 0.1 μL; or comprises no more than about 0.05 μL; or comprises no more than about 0.01 μL. 
     As used herein, the term “point of service location” may include locations where a subject may receive a service (e.g. testing, monitoring, treatment, diagnosis, guidance, sample collection, ID verification, medical services, non-medical services, etc.), and may include, without limitation, a subject&#39;s home, a subject&#39;s business, the location of a healthcare provider (e.g., doctor), hospitals, emergency rooms, operating rooms, clinics, health care professionals&#39; offices, laboratories, retailers [e.g. pharmacies (e.g., retail pharmacy, clinical pharmacy, hospital pharmacy), drugstores, supermarkets, grocers, etc.], transportation vehicles (e.g. car, boat, truck, bus, airplane, motorcycle, ambulance, mobile unit, fire engine/truck, emergency vehicle, law enforcement vehicle, police car, or other vehicle configured to transport a subject from one point to another, etc.), traveling medical care units, mobile units, schools, day-care centers, security screening locations, combat locations, health assisted living residences, government offices, office buildings, tents, bodily fluid sample acquisition sites (e.g. blood collection centers), sites at or near an entrance to a location that a subject may wish to access, sites on or near a device that a subject may wish to access (e.g., the location of a computer if the subject wishes to access the computer), a location where a sample processing device receives a sample, or any other point of service location described elsewhere herein. 
     Materials &amp; Methods 
     Described herein is the development of Streptavidin-conjugated superparamagnetic microparticles and it intermediates for immunoassay and biological materials separations. 
     In general, the superparamagnetic microparticles applications can be explained by the following example. The in-house developing ELISA assay which detects Zika IgM antibody in blood and cerebrospinal fluid (CSF).3 IgM antibody appears in blood a few days after Zika infection. A schematic of the ZIKV immunoassay is shown below in  FIG. 1 : 
       FIG. 1  shows a Schematic of ZIKV immunoassay 
     Streptavidin conjugated magnetic beads coated with biotinylated anti-human IgM antibody are incubated with serum or CSF. IgM antibodies in the blood bind to the beads, while unbound antibodies and serum components are removed by washing. Zika NS1 conjugated with alkaline phosphatase (Zika NS1-AP) is then added to the beads. Zika NS1-AP binds to human Zika-specific IgM antibodies and the amount of Zika NS1-AP bound to the beads are detected by using chemiluminescent substrate AMPPD. 
     The research and development of Streptavidin conjugated superparamagnetic microparticles and it intermediates are described. Those components are designed to be used as the magnetic separator or carrier for immunoassay and sample preparation purposes. Those usages included but not limited to (i) proteins, antibodies, antigens sample preparation and isolation. (ii) Cell separation. (iii) Protein-protein interaction studies. (iv) Immunoprecipitation. (v) Nucleic acid isolation and related sample preparation for PCR. (vi) IVD assay development. This document is intended to summarize and record the following aspects of the development. 
     Selection of the right polymers for the core assembly of microparticles. 
     Efforts to attain right size of monodispersed polymer spheres which is close to 2.8 μm diameter. This size is comparable to Dynabeads® M280 streptavidin-coupled. 
     Methods to introduce rigidity and chemical stability of the polymer particles to make them long shelf life and chemically inert for assay developments, robust in handling and stable in storage. 
     Efforts and ways to achieve the superparamagnetic properties of polymer microparticles by incorporating superparamagnetic iron (II, III) oxides, Fe3O4. 
     Functionalize the superparamagnetic polymer microparticles surface for conjugation purposes. 
     Streptavidin conjugation on the microparticles to assemble Streptavidin surface magnetic beads as the Final product for in-house applications. 
     Process development for the routine manufacturing which aimed to reduce batch to batch variability and provide more reliable and reproducible results for the purifications and analyses. 
     Pre-manufacturing verification and validation were done with MOP and MBR. 
     In-house development of superparamagnetic polymer microparticles are tube based concept. They are gentle and no columns or centrifugations are necessary. The true uniformity of bead size, shape and surface area should provide optimal accessibility and rapid liquid-phase reaction kinetics. In order to provide the insights for this characteristics, the quality control protocols are developed too. 
     The advantage and strength of the design for in-house superparamagnetic polymer microparticles are the concept of modular and ease of modification. This means the core, magnetic components, surface functionality and surface proteins, antibodies conjugation could be altered or modified without starting over from the beginning. These features could attribute to shorter research and product development cycles and faster turnover time with optimized cost effectiveness. 
     Definitions 
       
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                 Term 
                 Definition 
               
               
                   
               
             
            
               
                   
                 St 
                 Styrene monomer 
               
               
                   
                 AIBN 
                 2,2′-azobis(2-methylpropionitrile) 
               
               
                   
                 EtOH 
                 Ethanol or anhydrous ethanol 
               
               
                   
                 PAC 
                 Polyacrylic acid 
               
               
                   
                 Ar 
                 Argon inert gas 
               
               
                   
                 RCF 
                 Centrifuge Speed × g 
               
               
                   
                 DVB 
                 Divinylbenzene 
               
               
                   
                 GMA 
                 Glycidyl methacrylate 
               
               
                   
                 RT 
                 Room temperature 
               
               
                   
                 EDA 
                 Ethylene diamine 
               
               
                   
                 FeCl 2 •4H 2 O 
                 Iron (II) chloride tetrahydrate 
               
               
                   
                 FeCl 3 •6H 2 O 
                 Iron (III) chloride hexahydrate 
               
               
                   
                 NH3 
                 Ammonium hydroxide 
               
               
                   
                 PS 
                 Polystyrene 
               
               
                   
                 PGMA 
                 Polyglycidyl methacrylate 
               
               
                   
                 NH2 
                 Amine functional groups 
               
               
                   
                 Fe3O4 
                 Iron (II, III) oxide, superparamagnetic 
               
               
                   
                 CHO 
                 Aldehyde functional groups 
               
               
                   
                 BSA 
                 Bovine Serum Albumin 
               
               
                   
                 SA 
                 Streptavidin 
               
               
                   
                 NaN3 
                 Sodium azide 
               
               
                   
                 RLU 
                 Relative light units 
               
               
                   
                 QCP 
                 Quality control protocol 
               
               
                   
                 ELISA 
                 Enzyme-linked immunosorbent assay. 
               
               
                   
                 MOP 
                 Manufacturing operating procedure 
               
               
                   
                 MBR 
                 Manufacturing batch record 
               
               
                   
                 PBST 
                 Phosphate buffer saline with Tween 20 
               
               
                   
                 Biot-FAM 
                 Biotin-Fluorescein 
               
               
                   
                 DMSO 
                 Dimethyl sulfoxide 
               
               
                   
                 AP 
                 Alkaline phosphatase 
               
               
                   
                 AMPPD 
                 Chemilluminescence 1,2-dioxetane substrate  
               
               
                   
                   
                 for alkaline phosphatase 
               
               
                   
               
            
           
         
       
     
     Discussion 
     One embodiment of superparamagnetic polymer microparticles are designed to replace the commercial streptavidin-coupled beads. The present embodiment would open the new pathways for other superparamagnetic polymer microparticles applications, including separation and purification of biomolecules, MM contrasting agent, biosensors and drug target payload delivery.4 
     In order to incorporate the design for highest efficiency in product development cycles and faster turnover time with optimized cost saving, the concept of modular and ease of modification was implemented. This involves the ease of alteration and modification of the core, magnetic components, surface functionality and surface proteins, antibodies conjugation without starting over from the beginning. 
     One of the important features to make Dynabeads® products became to Gold Standard in superparamagnetic particles for Immunoassays development, separation and purification of biomolecules, is the uniform spherical and monodisperse stable particles.  FIG. 2  below illustrates magnetic particles from alternative suppliers often have a random size range and surface area that could compromise the reproducibility of experimental results. 
     If superparamagnetic particles production could be tightly controlled and yield the uniform spherical beads, with highly defined and consistent product characteristics. These would reducing particle variability, and getting more reliable and reproducible results. Also, this would reduce the batch-to-batch variable and enhance the robustness of the whole process. 
       FIG. 2  shows SEM-pictures of Dynabeads (A) and alternative magnetic particles from other suppliers (B-E). 
     The development of the one embodiment of superparamagnetic particles design input and specification be based in part on the specifications of Dynabeads® M280 Streptavidin beads.  FIG. 3  below is an example of a batch of Dynabeads®. 
       FIG. 3  shows Dynabeads specifications. 
     QCP were narrowed down to (i) Biotin Loading, (ii) Size analysis by Beckman Coulter Z2 Particle Counter with 50 μm aperature. Microscopic Imaging were served as in process QC.  FIG. 4  below showing the comparison of Dynabeads M280 SA beads and in-house developed SA beads with same magnification. 
       FIG. 4A  shows Dynabeads M280 SA beads (right) and in-house developed SA beads (left) with same magnification. 
     One Embodiment of the Procedure 
     One embodiment of Overall Pathway of Multistep Synthesis 
     See  FIG. 4B  for Scheme 1 comprising: Steps (1) Polymerization of Core-&gt;(2) Coating of Core-&gt;(3) Amination of Coating 
     See  FIG. 4C  for Scheme 2 comprising Steps (4) Iron(II,III) oxide Incorporation-&gt;(5) Surface activation from amino to aldehyde 
     See  FIG. 4D  for Scheme 3 comprising: Steps (6) Protein Conjugation via amino groups of protein with aldehyde of beads 
     The 3 schemes above showing the (1) core formation with special type of polymerization with styrene monomer. (2) PGMA coating on the PS seeds. (3) Amination of the epoxy function groups from PGMA coating. (4) Fe3O4 superparamagnetic nanoparticles incorporation inside the polymer microparticles. (5) Aldehyde functionalization with glutaraldehyde. (6) Final Streptavidin as a protein to conjugate to the surface aldehyde. 
     6.3 In this non-limiting example, the first step of the whole synthetic pathway for synthesis of in-house superparamagnetic microparticles was proved to be very critical in both design and implementation. Because of this, a lot of other conditions and strategies had been tried out in order to narrow down and finalize to this condition. The condition records below is the seeding method which can ensure the right size and highly monodispersed PS seeds to be prepared. For pre-manufacture synthesis, a 2 Liter jacketed reactor equipped with overhead stirrer have been selected. ( FIG. 5 ) 
       FIG. 5  shows one embodiment of a 2 Liter jacketed reactor equipped with overhead stirrer for Seeds polymerization in Step 1. 
     The following table summarizes the component of the reagents needed to start the optimized polymerization for PS core seeds. The manufacturing operating procedure was recorded in MOP-00807-F1.6 All of the reagents were dissolved in the ethanol-water mixture and purged with Ar in the reactor. The overhead stirrer was set to 500 rpm and circulator temperature of the jacket was adjusted to about 70° C. The polymerizations were carried out for overnight. Once they are done, the reactor temperature was ramped down to room temperature and the contents were transfer from the reactor to centrifuge tubes. Centrifuge the material at 1500 rcf, and disposed of the supernatant, repeat the washing procedure with fresh ethanol for seven times. 
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                 Item (Step1) 
                 Amount (100 ml St) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 St 
                 100  
                 ml 
               
               
                   
                 AIBN 
                 0.89  
                 g 
               
               
                   
                 EtOH anhyd (4 L bottle EMD, EX0285-3) 
                 700 
                 ml 
               
               
                   
                 DI water 
                 14 
                 ml 
               
               
                   
                 PAC 450 k Da 
                 1.04  
                 g 
               
               
                   
               
            
           
         
       
     
     Purification or separation of the PS seeds was relied on the centrifuge. Once it was done, the seeds were dried in vacuum oven at 50 C with 30 in Hg vacuum pressure. In process microscopic images showing the uniform monodisperse particle with ˜2.2 μm in reproducible manner for all the batches made. 
     6.4 In this non-limiting example, the second step of the preparation is PGMA coating on the seeds. The following table summarizes the materials needed in this step. Seeding method was chosen rather than using styrene, DVB, and GMA co-polymerization at single step is because the previous experiments proved the monodispersion uniform spherical particles from polymerization were only achieved by seeding methods.  FIG. 6  showing co-polymerization particles microscopic image with same 800× magnification. All of them were suffered by non-spherical shape, multiple sizes of the beads formed at the same polymerization batch or sizes are much larger than 2.8 μm. 
     
       
         
           
               
               
               
             
               
                   
               
               
                 Item (Step2) 
                 R&amp;D procedure 
                 Pre-manufacture procedure 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 PS Seed 
                 0.5 
                 g 
                 5 
                 g 
               
            
           
           
               
               
               
            
               
                 St 
                 0 
                 0 
               
            
           
           
               
               
               
               
               
            
               
                 DVB 
                 25  
                 ul 
                 0.25 
                 ml 
               
               
                 GMA 
                 0.5 
                 m1 
                 5 
                 ml 
               
               
                 AIBN 
                 15 
                 mg 
                 150 
                 mg 
               
            
           
           
               
               
               
            
               
                 EtOH anhyd 
                 16 ml + 2 ml GMA/ 
                 160 + 20 
               
            
           
           
               
               
               
               
            
               
                   
                 EtOH adsorption 1 h 
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                 DI water 
                 1 
                 ml 
                 25 
                 ml 
               
               
                 PAC 450 k Da 
                 30 
                 mg 
                 400 
                 mg 
               
               
                   
               
            
           
         
       
     
       FIG. 6  shows co-polymerization particles microscopic images with same 800× magnification. 
     Therefore, coating of PGMA on the PS seeds recorded in the above table of materials was the optimized set of selection. Coating procedures were routinely done by pre-suspending PS seeds in ethanol-water mixture, followed with DVB, GMA, AIBN mixture in ethanol slowly dropwise addition to seeds suspension. After purging with Ar and stirring for an hour, the mixture was started heating with 120° C. hot plate setting, and internal temperature reached 80° C. The coating needed 6 h to complete. If the reaction time was not enough, the coating would not complete and the next couple steps would yield the bead suspension prone to be aggregation. 
     Once the reactions were done, the contents were cool down to room temperature and separated by centrifuge with 1000 rcf. The supernatant was disposed, and the beads were washed with fresh ethanol and repeat the separation with centrifuge at 1000 rcf for 5 times. The brief centrifugation served as the most convenient choices of purification in those processes, and this selection makes the processes readily scale up and enhance the speed and low costs of the preparations. Moreover, the coating quality could be verified by the upcoming steps immediately. Once the PGMA coated beads were separated, they were subjected to next step directly without drying. 
     6.5 In this non-limiting example, the third step of the preparation is amination of the coated beads. Table below showing the materials in optimized amounts used in this reaction step. Largely excess of ethylene diame (EDA) was used to react with the epoxy functional groups on the PGMA coating. The condition was finely optimized and avoid the intermolecular reaction could be happened and causing aggregation of the beads. In general, coated beads were suspended in ethanol and the DEA in ethanol was added in one shot to the beads suspension. Stirring and sonication would be the best combination of agitation. Once the stirring of the reaction mixture was done by overnight at room temperature. The aminated surface beads were separated and washed with ethanol with the help of centrifuge at 2200 rcf. After 6 times of repeated washings, the beads were dried in vacuum oven as the description of related MOP00809-F1.8 In this and last step, the preparation were done on the 500 to 1000 ml Erlenemeryer flasks with screw cap for Ar purging capability, see  FIG. 7 . 
       FIG. 7  shows one embodiment of Erlenemeryer flasks for coating and amination steps. 
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                 Item (Step3) 
                 2.2 um Beads Amination 
               
               
                   
               
             
            
               
                   
                 PGMA coated Seeds 
                 5 g 
               
               
                   
                 DEA 
                 12 ml in 36 ml EtOH 
               
               
                   
                 EtOH anhyd 
                 180 ml EtOH for 
               
               
                   
                   
                 PGMA seeds suspension 
               
               
                   
               
            
           
         
       
     
     6.6 Iron oxide incorporation step is the one to make the polymer microparticles become superparamagnetic. Many different approaches had been tried out in order to get the superparamagnetic nanoparticles of Iron (II, III) oxides (Fe3O4) entrapped inside the polymers. Following  FIG. 8  illustrates the comparison of the two major methods used. Which is (1) swelling and entrapping and (2) precipitation methods. At the end, the precipitation was selected and optimized here for the preparation.9 Swelling and entrapping method was not able to bring enough amount of Fe inside the beads and which shown in the  FIG. 8  with pale brown color verse brown color of precipitation method. Meanwhile, swelling method require using toluene as the solvent which require higher amount of DVB to keep the rigidity and chemical stability of the beads structure. Higher amount of DVB would cause the monodispersed uniform beads harder to form. Precipitation method used here was also able to yield different loading of the Fe content and which was fine tuned to match the magnetic response in order to achieve similar performance under the same strength of magnets.  FIG. 9  showing different loading of the Fe and the color changes. 
       FIG. 8  shows results of beads from swelling and entrapping (left), and precipitation (right) methods. 
       FIG. 9  shows color different of Fe precipitation 1× (left), and 2× ratio (right). 
     The following table summarizes the optimized amounts of the reagents used in Fe3O4 incorporation step. Higher Fe loading is not essentially to achieve the best performance as the deeper color would absorb the chemiluminescence radiation emitted by AMPPD substrate of AP. 
     
       
         
           
               
               
               
               
             
               
                   
               
               
                   
                   
                 Fe(II, III)  
                   
               
               
                   
                   
                 oxide in Amino 
                 Pre-manufacture  
               
               
                   
                 Item (Step4) 
                 Beads R&amp;D 
                 batches 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 FeCl3.6H2O 
                 250 
                 mg 
                 3.9 
                 g 
               
               
                   
                 FeCl2.4H2O 
                 120 
                 mg 
                 1.9 
                 g 
               
               
                   
                 DI water for 
                 3 
                 ml 
                 40 
                 ml 
               
               
                   
                 FeCl x   
                   
                   
                   
                   
               
               
                   
                 dissolution 
                   
                   
                   
                   
               
               
                   
                 NH3 
                 3 
                 ml 
                 47 
                 ml 
               
               
                   
                 Amino Beads 
                 490 
                 mg 
                 6.2 
                 g 
               
               
                   
                 DI water for 
                 15 
                 ml 
                 190 
                 ml 
               
               
                   
                 beads 
                   
                   
                   
                   
               
               
                   
                 suspension 
               
               
                   
               
            
           
         
       
     
     Amination beads were first suspended in the deionized water, if the coating of PGMA was not complete, the beads starting material with amine surface would not enough to make them suspended well in aqueous. This important observation was serving as one of the in process QC, and which can stop the process moving forward by itself if the thing went wrong. This design is sophisticated as the amine surface is essential to provide the hydrophilic environment of the beads for the Fe2+, Fe3+ ions to penetrate the polymer surface. Meanwhile, the amine surface is required for the beads to well suspend in the aqueous for this step of reaction to go forward. Once the Fe ions go inside the beads, ammonia added will start precipitate the Fe ions to form Fe 3 O 4  nanoparticles. These particles trap inside the polymer makes this preparation complete. 
     
       
         
           
               
               
             
               
                   
               
               
                   
                 Surface activation of 2.2 um 
               
               
                 Item (Step 5) 
                 Fe(II, III) oxide incorp Beads 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 Fe(II, III) Oxide incorp Beads 
                 1 
                 g 
               
               
                 50% Glutaraldehyde 
                 3.5 
                 ml 
               
               
                 DI water 
                 28 
                 ml 
               
               
                   
               
            
           
         
       
     
       2Fe 3+ (aq)+Fe 2+ (aq)+8OH − →Fe 3 O 4 (s)+4H 2 O
 
     In general, the procedure was carried out by suspending amine surface beads in deionized water as above ratio. Adding the beads suspension into the dissolved Fe2+, Fe3+ solution, and stirred for 2 hours to allow the Fe ions penetration inside the polymer beads. Purging of the Ar was needed to avoid the other form of iron oxides formation. After stirring for 2 h, ammonia 28-30% w/w was added dropwise and the mixture was agitated with stirring and sonication alternatively. Once the addition was finished, the mixture was heated to 80° C. with hotplate set to 130° C. and stirred for 2.5 h. At the end, the content was cool down to room temperature and the Iron (II, III) oxide incorporated beads were washes with deionized water and separated with centrifuge at 2500 rcf for 10 to 15 times until no color supernatant remained. Magnetic separation could be served as a replacement of centrifuge, however, the high throughput magnetic device is needed in order to achieve this grams-scale of preparation. In this step, the dried beads could be stored at freezer −18° C. for 52 weeks shelf life. 
     6.7 Aldehyde surface activated beads preparation was done by suspending the dried Fe3O4 incorporated beads and reacted with largely excess glutaraldehyde solution.10 Table below summarizes the optimized condition. For the planning of the streamlining manufacturing, 1.4-1.5 gram of the dried Fe3O4 incorporated beads could be stored in a 50 ml Falcon tube for each reaction, and this would be all the way carried down to last step: Streptavidin loading. ( FIG. 10 ) 
       FIG. 10  shows aliquoted plans for the stockpile of beads intermediates. Every tube of 50 ml Flacon tube can serve as a unit for the preparation all the way down to Streptavidin beads. 
     Once the reaction completed in 1.5 h, the aldehyde activated beads were washed with deionized water to remove excess glutaraldehyde. Again centrifuge at 2500 rcf was used for the separation, and this set-up can ensure the best use of instrument and simplify the process. CHO beads could be subjected to next step without drying and the loss of the beads during separation is minimal. 
     6.8 Streptavidin conjugated beads were done by simply adding the right amount of Streptavidin into the deionized water suspended CHO activated particles. The design of this step was aimed to simplify the conjugation step and make it available for non-trained workers. CHO activated particles are stable and highly activate towards amines side chain of the proteins including streptavidin here. The control of the loading could be possible by adjust the level of protein addition. At the end, BSA was added for covalent binding to the rest of CHO groups or non-covalent binding to the surface of protein. Final formulation was kept as 1% w/v of beads in deionized water with 0.02% NaN3 as an anti-microbial additive. Following table indicated the R&amp;D and pre-manufacturing phase condition of this step. Process development and pre-manufacturing condition increase amount of Streptavidin used in order to maximum the loading and driving the kinetic forward. 
       FIG. 11  is the nanodrop UV-Vis illustrate the initial and final A280 signal from free streptavidin in the solution. 
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                 Streptavidin Conjuagtion 
                   
               
               
                   
                 of 2.2 um surface activated 
                   
               
               
                 Item (Step6) 
                 beads (R&amp;D) 
                 Pre-manufacturing 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Beads 
                 150 
                 mg 
                 1 
                 g 
               
               
                 Streptavidin 
                 1.36 
                 mg 
                 33.3  
                 mg 
               
            
           
           
               
               
               
            
               
                 DI water 
                 3 ml (for beads) + 
                 29 ml + 5 ml 
               
               
                   
                 1 ml (for Streptavidin) 
                   
               
            
           
           
               
               
               
               
               
            
               
                 5% BSA in 
                 200 
                 u1 
                 570 
                 ul 
               
               
                 1 × PBS 
               
               
                   
               
            
           
         
       
     
       FIG. 11  shows nanodrop UV-Vis illustrate the initial and final A280 signal from free streptavidin in the solution. 
     When 33.3 mg of Streptavidin lyophilized powder was weighed out and dissolved in 5 ml of deionized water, A280 was measured as 0.868 by 2 fold dilution. Which is equal to 5.43 mg/ml. 4 ml of 5.43 mg·ml Streptavidin solution added makes up 21.75 mg initial. No buffer is needed because the lyophilized protein comes with salt there.  FIG. 11  indicates final A280 remained essentially the same after 1 h 45 min. 
     Verification and Validation 
     Verification and validation of the preparation process were done in pre-manufacturing phase with MOP and MBR of each step ready. The following  FIG. 12  illustrates the diagram of the first triplicate batches all the way from 3 separated batches of Starting PS seeds carried down parallel to the final SA-beads. The microscopic images were recorded and compared to the previous format of process development done by Adam Mann (A Senior Scientist who failed in the process development of this project for 2 years).12 The new processes described here demonstrate every step of intermediates behave much closer to R&amp;D results. Monodisperses and uniformity of beads are greatly improved when compare the Images (Microscope 800×) from  FIG. 13  (Current Process Development described here) and  FIG. 14  (Previous Failure of the process done by another scientist in past 2 years) for every step. 
       FIG. 12  shows triplicate batches done with L/N for verification and validation. 
       FIG. 13  shows triplicate batches done with L/N for verification and validation observed in microscopic image with 800× magnification. 
       FIG. 14  shows previous process development batches done with L/N observed in microscopic image with 800× magnification. 
     Besides the triplicate verification and validation, the following  FIG. 15  describes more batches were generated. The extra runs provided more information for the improvement of the processes and the tolerance of the conditions which is important to ensure the reproducibility and robustness of the preparations. Also, based on the collected data both in R&amp;D and process re-development here, the shelf lives of the following items were extended. This could improve the overall effectiveness of the whole projects without compromising the quality of deliveries. 
     
       
         
           
               
               
               
               
             
               
                   
               
               
                 P/N 
                 02-00405 
                 02-00407 
                 02-00408 
               
               
                   
               
             
            
               
                 Product Name 
                 Polystyrene  
                 Aminated  
                 Iron (II, III) oxide 
               
               
                   
                 Core Seeds 
                 Coated Seeds 
                 Incorporated Beads 
               
               
                 Previous shelf life 
                 RT 25 W 
                 RT 25 W 
                 RT 20 W 
               
               
                 Suggested change 
                 RT 104 W 
                 &lt;4° C. 52 W 
                 &lt;4° C. 52 W 
               
               
                   
               
            
           
         
       
     
       FIG. 15  shows more batches done with L/N for verification and validation. 
       FIG. 15  recorded the S000008460 batch ended up with aggregation. This finding was finally resolved by using another lot number of GMA reagent from Sigma Aldrich used to prepare PGMA coating step. Original L/N MKBT8176V proved to be not as good as MKCB8502 from the vender. Although the same L/N of reagent has been used before, however, they are from different bottles and opened at different time. Besides, amount of water used in PGMA coating step was further adjusted in order to improve the result. Following table showing the adjustment of couple of parameters and conditions to make sure the reproducibility of the processes. S000008509 and S000008511 were 2 batches of Fe3O4 incorporated beads stockpiled here for later usage. 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                 P/N 
                 Name 
                 Condition A 
                 Condition B 
                 Condition C 
                 Best 
               
               
                   
               
             
            
               
                 02-00406 
                 Coated beads 
                 1 h/2 h -&gt; X 
                 1 h/4 h -&gt; X 
                 1-1.5 h/5.5-6 h -&gt; 
                 C 
               
               
                   
                   
                   
                   
                 Good 
                   
               
               
                 02-00406 
                 Coated beads 
                 20 ml water 
                 25 ml water 
                 0.5 ml water-&gt; 
                 B 
               
               
                   
                   
                 (5 g scale)-&gt; 
                 (5 g scale)-&gt; 
                 failed 
                   
               
               
                   
                   
                 ok 
                 good 
                   
                   
               
               
                 02-00407 
                 Amined 
                 1× diamine-&gt; 
                 1.5× diamine-&gt;  
                 1.2× diamine-&gt; 
                 C 
               
               
                   
                   
                 good 
                 good 
                 good 
                   
               
               
                 02-00408 
                 Fe3O4—NH2 
                 1 h/2 h-&gt;  
                 2 h/2.5 h-&gt; 
                 2 h/ 2 h-&gt;  
                 C 
               
               
                   
                   
                 good 
                 good 
                 good 
                   
               
               
                 03-01116 
                 SA 
                 6 mg SA: 
                 15 mg SA: 
                 24 mg 
                 C (make sure 
               
               
                   
                   
                 700 mg  
                 700 mg  
                 SA: 700 mg-&gt; 
                 highly 
               
               
                   
                   
                 02-00426  
                 02-00426-&gt;  
                 ok 
                 loaded) 
               
               
                   
                   
                 R&amp;D phase)-&gt;  
                 ok 
                   
                   
               
               
                   
                   
                 ok 
                   
                   
                   
               
               
                 03-01116 
                 SA 
                 0.003% NaN3 
                 0.02% NaN3 
                   
                 B (shared w. 
               
               
                   
                   
                 in DI water 
                 in DI water 
                   
                 Dyna spec) 
               
               
                   
               
            
           
         
       
     
     In one non-limiting example, the method may comprise the following: 
     Step1: Forming polystyrene micron particle without crosslinker from styrene monomer (2 um). 
     Step2: Coating of polystyrene micron particles with PGMA (polyglycidyl methacrylate) layer and crosslinked with DVB (Divinylbenzene) (2.8 um) all the steps until last. 
     Step3: Amination of the epoxy surface from Step 2 product (PGMA layer) 
     Step4: Using the amine surface hydrophilicity to allow Ferric and Ferrous Chloride to penetrate inside the beads and forming precipitate that trapped inside the beads by reacting Ferric and Ferrous Chlordie with ammonia. 
     Step5: Using glutaraldehyde to react with amine surface and form the aldehyde activated surface for next step. 
     Step6: react aldehyde surface with protein lysine side chain (here is streptavidin in our product) to lead to streptavidin conjugated beads. (2.8 um) 
     8. Qualitative Analysis Development 
     Quality control of the in-house developed Streptavidin conjugated beads were done by 2 major protocols developed by the team based on the specification of Dynabeads® M280 SA beads. 
     First QCP was aimed to determine the Streptavidin density or surface binding capacity of the loaded beads.13 This protocol was used to compare a batch of Dynbeads® which claimed to have the certain loading capacity.  FIG. 16A ,  FIG. 16B  illustrate the chemical structure of Biotin-Fluorescein used in this QCP and way of binding with streptavidin respectively. 
       FIG. 16A  shows a chemical structure of Biot-FAM. 
       FIG. 16B  shows a way of binding between Biot-FAM with Streptavidin. 
     In the procedure, a 1/251 dilution of Biotin-Fluorescein conjugate of 5 mg/ml in DMSO with 10 mM Phosphate Buffer Saline (PBS) with 0.05% Tween 20 was made. 100 μL of this diluted Biotin-Fluorescein solution was added to 100 μL of suspended Streptavidin Beads 1% w/v, and vortex at room temperature for 30 min. Meanwhile, the Controls were prepared at the same time by adding 100 μL of diluted Biotin-FAM to 100 μL of 10 mM Phosphate Buffer Saline (PBS) with 0.05% Tween 20. After 30 min incubation, the Samples and Controls were placed in the DynaMag-2 Magnetic separator. 150 μL of each tube were transferred to cuvettes for measurement of changes of A495. 
     Based on the QCP-00194-F1, the following batches were determined and the results were reported as following: 
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 Document Number: 
                 QCP-00194-F2 
               
               
                   
                 Test Date: 
                 21 Mar. 2017 
               
               
                   
                 Operator: 
                 Omid Khakshoor 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 Document Revision: 
                 01 
               
               
                   
                 Release Date: 
                 DDMMMYYYY 
               
               
                   
                 QC ID: 
                 S000008445 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
            
               
                   
               
               
                 Tested Reagents Info 
               
            
           
           
               
               
               
               
            
               
                 Description 
                 Part Number 
                 Lot Number 
                 DOE 
               
               
                   
               
               
                 Streptavidin-conjugated 
                 03-01116 
                 S000008445 
                 12 Jun. 2017 
               
               
                 Beads, 1% w/v Stock 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
            
               
                   
               
               
                   
                   
                   
                   
                 μg/mL  
                 nmol/mg  
                   
                   
               
               
                   
                 Abs @ 495 nm 
                   
                 Δ abs @  
                 Biotin- 
                 Biotin 
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 Sample name 
                 1 
                 2 
                 Average 
                 495 nm 
                 Fluorescein 
                 Fluorescein 
                 % CV 
                 Pass/Fail 
               
               
                   
               
               
                 CONTROL 
                 0.7640 
                 0.7680 
                 0.7660 
                   
                   
                   
                   
                   
               
               
                 SAMPLE 
                 0.5800 
                 0.5780 
                 0.5790 
                 0.1870 
                 1.789 
                 0.488 
                 0.24% 
                 PASS 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 Document Number: 
                 QCP-00194-F2 
               
               
                   
                 Test Date: 
                 21 Mar. 2017 
               
               
                   
                 Operator: 
                 Omid Khakshoor 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 Document Revision: 
                 01 
               
               
                   
                 Release Date: 
                 DDMMMYYYY 
               
               
                   
                 QC ID: 
                 S000008446 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
            
               
                   
               
               
                 Tested Reagents Info 
               
            
           
           
               
               
               
               
            
               
                 Description 
                 Part Number 
                 Lot Number 
                 DOE 
               
               
                   
               
               
                 Streptavidin-conjugated 
                 03-01116 
                 S000008446 
                 12 Jun. 2017 
               
               
                 Beads, 1% w/v Stock 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
            
               
                   
               
               
                   
                   
                   
                   
                 μg/mL  
                 nmol/mg  
                   
                   
               
               
                   
                 Abs @ 495 nm 
                   
                 Δ abs @  
                 Biotin- 
                 Biotin- 
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 Sample name 
                 1 
                 2 
                 Average 
                 495 nm 
                 Fluorescein 
                 Fluorescein 
                 % CV 
                 Pass/Fail 
               
               
                   
               
               
                 CONTROL 
                 0.7640 
                 0.7680 
                 0.7660 
                   
                   
                   
                   
                   
               
               
                 SAMPLE 
                 0.5150 
                 0.5100 
                 0.5125 
                 0.2535 
                 2.425 
                 0.662 
                 0.69% 
                 PASS 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 Document Number: 
                 QCP-00194-F2 
               
               
                   
                 Test Date: 
                 21 Mar. 2017 
               
               
                   
                 Operator: 
                 Omid Khakshoor 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 Document Revision: 
                 01 
               
               
                   
                 Release Date: 
                 DDMMMYYYY 
               
               
                   
                 QC ID: 
                 S000008447 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
            
               
                   
               
               
                 Tested Reagents Info 
               
            
           
           
               
               
               
               
            
               
                 Description 
                 Part Number 
                 Lot Number 
                 DOE 
               
               
                   
               
               
                 Streptavidin-conjugated 
                 03-01116 
                 S000008447 
                 12 Jun. 2017 
               
               
                 Beads, 1% w/v Stock 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
               
            
               
                   
               
               
                   
                   
                   
                   
                   
                 μg/mL  
                 nmol/mg  
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 Abs @ 495 nm 
                   
                 Δ abs @  
                 Biotin- 
                 Biotin- 
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 Sample name 
                 1 
                 2 
                 Average 
                 495 nm 
                 Fluorescein 
                 Fluorescein 
                 % CV 
                 Pass/Fail 
               
               
                   
               
               
                 CONTROL 
                 0.7640 
                 0.7680 
                 0.7660 
                   
                   
                   
                   
                   
               
               
                 SAMPLE 
                 0.5640 
                 0.5630 
                 0.5635 
                 0.2025 
                 1.937 
                 0.529 
                 0.13% 
                 PASS 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 Document Number: 
                 QCP-00194-F2 
               
               
                   
                 Test Date: 
                 31 Mar. 2017 
               
               
                   
                 Operator: 
                 Omid K 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 Document Revision: 
                 01 
               
               
                   
                 Release Date: 
                 DDMMMYYYY 
               
               
                   
                 QC ID: 
                 S000008475 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
            
               
                   
               
               
                 Tested Reagents Info 
               
            
           
           
               
               
               
               
            
               
                 Description 
                 Part Number 
                 Lot Number 
                 DOE 
               
               
                   
               
               
                 Streptavidin-conjugated 
                 03-01116 
                 s000008475 
                 23 Jun. 2017 
               
               
                 Beads, 1% w/v Stock 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
               
            
               
                   
               
               
                   
                   
                   
                   
                   
                 μg/mL  
                 nmol/mg  
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 Abs @ 495 nm 
                   
                 Δ abs @  
                 Biotin- 
                 Biotin- 
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 Sample name 
                 1 
                 2 
                 Average 
                 435 nm 
                 Fluorescein 
                 Fluorescein 
                 % CV 
                 Pass/Fail 
               
               
                   
               
               
                 CONTROL 
                 0.7205 
                 0.7260 
                 0.7233 
                   
                   
                   
                   
                   
               
               
                 SAMPLE 
                 0.4760 
                 0.4770 
                 0.4765 
                 0.2468 
                 2.361 
                 0.644 
                 0.15% 
                 PASS 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 Document Number: 
                 QCP-00194-F2 
               
               
                   
                 Test Date: 
                 3 Apr. 2017 
               
               
                   
                 Operator: 
                 Alex Lee 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 Document Revision: 
                 01 
               
               
                   
                 Release Date: 
                 DDMMMYYYY 
               
               
                   
                 QC ID: 
                 S000008482 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
            
               
                   
               
               
                 Tested Reagents Info 
               
            
           
           
               
               
               
               
            
               
                 Description 
                 Part Number 
                 Lot Number 
                 DOE 
               
               
                   
               
               
                 Streptavidin-conjugated 
                 03-01116 
                 s000008482 
                 26 Jun. 2017 
               
               
                 Beads, 1% w/v Stock 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
               
            
               
                   
               
               
                   
                   
                   
                   
                   
                 μg/mL  
                 nmol/mg  
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 Abs @ 495 nm 
                   
                 Δ abs @  
                 Biotin- 
                 Biotin- 
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 Sample name 
                 1 
                 2 
                 Average 
                 495 nm 
                 Fluorescein 
                 Fluorescein 
                 % CV 
                 Pass/Fail 
               
               
                   
               
               
                 CONTROL 
                 0.7205 
                 0.7295 
                 0.7250 
                   
                   
                   
                   
                   
               
               
                 SAMPLE 
                 0.5010 
                 0.5105 
                 0.5058 
                 0.2193 
                 2.097 
                 0.572 
                 1.33% 
                 PASS 
               
               
                   
               
            
           
         
       
     
     Besides on those batches with L/N above, during process re-development, couples of batches were tested on Biotin loading and compared with a lot of Dynabeads® M280 SA (L/N 160804110) beads as below: 
     
       
         
           
               
               
               
               
               
               
            
               
                   
               
               
                   
                 Abs @ 495 nm 
                   
                 Δ abs @  
                 μg/mL  
                 nmol/mg  
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Sample name 
                 1 
                 2 
                 avg 
                 495 nm 
                 Biotin- 
                 Biotin- 
               
               
                   
               
               
                 CONTROL 
                 0.7580 
                 0.7500 
                 0.7540 
                   
                   
                   
               
               
                 06mar2017-A 
                 0.4850 
                 0.4920 
                 0.4885 
                 0.2655 
                 2.540 
                 0.693 
               
               
                 06mar2017-B 
                 0.5320 
                 0.5340 
                 0.5330 
                 0.2210 
                 2.114 
                 0.577 
               
               
                 Dynabead  
                 0.59 
                 0.584 
                 0.5870 
                 0.1670 
                 1.598 
                 0.436 
               
               
                 160804110 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
            
               
                   
               
               
                   
                 Abs @ 495 nm 
                   
                 Δ abs @  
                 μg/mL Biotin- 
                 nmol/mg Biotin- 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Sample name 
                 1 
                 2 
                 avg 
                 495 nm 
                 Fluorescein 
                 Fluorescein 
               
               
                   
               
               
                 CONTROL 
                 0.6970 
                 0.7010 
                 0.6990 
                   
                   
                   
               
               
                 2-Mar 
                 0.4360 
                 0.4580 
                 0.4470 
                 0.2520 
                 2.411 
                 0.658 
               
               
                   
               
            
           
         
       
     
     In-house developed Streptavidin conjugated beads with Biotin loading ranged from 488 to 693 pmol/mg. Every batch synthesized here is still larger than Dynabeads® M280 SA (L/N 160804110) 436 pmol/mg. Specification of Dynabeads® this lot numbers was tested as 756 pmol/mg with 14C conjugated Biotin and Dynabeads® tested different lots could ranged from 650-900 pmol/mg. When comparing the figures, the in-house beads are very close to the range of variable with Dynabeads® M280, SA conjugated, and with a higher loading of biotin/Streptavidin surface for every batches synthesized so far. 
     QC pass/fail criteria was set as any loading between 450-700 pmol/mg and % CV less than 5% as PASS. 
     Another QCP-00202-F1 developed at Theranos for the in-house Streptavidin conjugated beads is using Beckman Coulter Z2 Particle Counter with 50 μm aperture to determine the size and distribution.14 At first, 10 mL of Electrolyte solution was dispensed to 15 mL Falcon tubes. 100 μl of Sample beads in 1% w/v was added and mixed well with the Electrolyte solution. Then, 1 mL of the diluted beads solution was transferred to a 10 mL of Electrolyte solution in Accuvate vials. These series of dilution ended up as 1000 fold dilution of 1% w/v beads. QC pass/fail criteria was set as any sizes between 2.4 to 3.3 μm and % CV less than 13% as PASS.  FIGS. 16C-16J  show results from batches prepared here for sizing quality control (QC). 
     Dynabeads® M280 SA (L/N 160804110) 
     Description 
     P/N 02-00405 One embodiment of Polystyrene Core Seeds has been successfully developed in-house using carefully controlled and optimized condition for AIBN radical polymerization from styrene monomer, with catalytic amount of polyacrylic acid PAC and water in ethanol solution. This development can deliver monodispersed, uniform spherical beads with size controllable. Result are promising and able to compare with Gold Standard from Industry-Dynabeads®.  FIG. 17  showing the microscopic image of the Seeds and Dynabeads reference (800×). 
       FIG. 17  shows in-house PS seeds (left), Dynabeads M280, sized ˜2.8 μm with SA surface (right). 
     P/N 02-00406 One embodiment of Coated Seeds has been developed with PGMA coating on top of the PS seeds formed from last step. This process is unique and well controlled. PGMA coating allow the same method to be employed on wide range of seeds sizes without compromising the quality and redevelop on other conditions. This step was chosen because other attempts demonstrated co-polymerization of St, DVB, GMA could not easily yield monodispersed beads. With coating method on the seeds surface, this solve both issues of sizes monodisperse control and functional surface introduction by single step. Moreover, this step allows quick in-process checking for well completion of PGMA coating. If the amine surface of next step could not dispense well in aqueous, it would stop the process moving forward.  FIG. 18  showing difference of Successful seeds coating and co-polymerization images. 
       FIG. 18  shows one embodiment of in-house PS-PGMA coated seeds (left), co-polymerization formed PS-PGMA beads (right). 
     P/N 02-00407 One embodiment has been developed with simple mixing of PGMA coated seeds with excess of ethylene diamine. This step increase the hydrophilic property of the beads surface and served as the in-process QC step for PGMA coating completion. If the beads could not disperse well in aqueous, which formed foaming mixture and indicated the coating step went wrong. This mixture could not continue for next step because of the mixing issue. In most cases, the microscopic image can tell the difference. ( FIG. 19 ) 
       FIG. 19  shows one embodiment of in-house aminated beads (left), aminated beads with incomplete PGMA coating in previous step (right). 
     P/N 02-00408 One embodiment of Iron (II, III) oxide incorporated beads has been prepared based on optimized condition developed at Theranos. This precipitation was selected instead of swelling entrapping method mentioned above because of the controllable loading and less DVB needed for crosslinking. Also, swelling method was not able to perform the high loading of Fe content compare to the current incorporation with precipitation.  FIG. 20  records the color or Iron content difference between 2 mentioned methods. Less color means less Iron incorporate and the magnetic responds are much slower in same magnetic field. 
       FIG. 20  shows one embodiment of in-house Fe3O4 beads with swelling-entrapping (left), in-house beads with precipitation to incorporate Fe3O4 (right). 
     P/N 02-00426 One embodiment of Aldehyde Surface Activated beads were developed for the quick conjugation of protein free amine or lysine side chain to surface aldehyde. This intermediate was done by suspending Iron (II, III) oxide incorporated beads in excess of glutaraldehyde 50% in water. This step was planned to be happened immediately before the Streptavidin conjugation to avoid any degrading of activity of aldehyde for conjugation. No drying of the product beads is needed although it is okay to dry and stored for months. 
     P/N 03-01116 One embodiment of Streptavidin conjugated beads finally deliver as 1% w/v formulation with 0.02% NaN3 added as antimicrobial. Conjugation of Streptavidin or other proteins antibodies could be possible too. This step was designed for un-trained workers, as a result, the operation is as simple as 1-2-3. First add the right amount of excess of streptavidin into the aldehyde surface beads which is highly activated towards amines from protein lysine side chains, yet stable. Second, waits for 1-2 hr, then blocking with BSA. Thirdly, cleaning with magnetic separation or centrifuge with deionized water washings. 
     By way of non-limiting example, all of the six steps designed and developed for this beads project could deliver a well sized, uniform and monodisperse microparticles perform similar to Dynabeads®. Intermediates could be served as individual products for other application. Such as P/N 02-00408, amine surface Iron (II, III) oxide incorporate beads can be used to react with succinimde activated antibodies, antigens for coating. And P/N 02-00426 aldehyde surface activated beads could be react with proteins A/G for suitable antibodies purification applications. They implement the design idea of Modular, and benefits the shorter design cycles and effectiveness in resources saving. 
     The Flow chart in  FIG. 21  summarizes one embodiment of the process of Streptavidin conjugated beads and its intermediates preparation: 
     While the invention has been described and illustrated with reference to certain particular embodiments thereof, those skilled in the art will appreciate that various adaptations, changes, modifications, substitutions, deletions, or additions of procedures and protocols may be made without departing from the spirit and scope of the invention. For example, with any of the above embodiments, it should be understood that although whole blood may be the sample used, other types of sample such as saliva, mucus, etc. . . . may also be used. 
     Additionally, concentrations, amounts, and other numerical data may be presented herein in a range format. It is to be understood that such range format is used merely for convenience and brevity and should be interpreted flexibly to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as if each numerical value and sub-range is explicitly recited. For example, a size range of about 1 nm to about 200 nm should be interpreted to include not only the explicitly recited limits of about 1 nm and about 200 nm, but also to include individual sizes such as 2 nm, 3 nm, 4 nm, and sub-ranges such as 10 nm to 50 nm, 20 nm to 100 nm, etc. . . . 
     The publications discussed or cited herein are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. Further, the dates of publication provided may be different from the actual publication dates which may need to be independently confirmed. All publications mentioned herein are incorporated herein by reference to disclose and describe the structures and/or methods in connection with which the publications are cited. U.S. Provisional Application Ser. No. 62/577,680 filed Oct. 26, 2017 is fully incorporated herein by reference for all purposes. 
     While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. Any feature, whether preferred or not, may be combined with any other feature, whether preferred or not. The appended claims are not to be interpreted as including means-plus-function limitations, unless such a limitation is explicitly recited in a given claim using the phrase “means for.” It should be understood that as used in the description herein and throughout the claims that follow, the meaning of “a,” “an,” and “the” includes plural reference unless the context clearly dictates otherwise. For example, a reference to “an assay” may refer to a single assay or multiple assays. Also, as used in the description herein and throughout the claims that follow, the meaning of “in” includes “in” and “on” unless the context clearly dictates otherwise. Finally, as used in the description herein and throughout the claims that follow, the meaning of “or” includes both the conjunctive and disjunctive unless the context expressly dictates otherwise. Thus, the term “or” includes “and/or” unless the context expressly dictates otherwise.