Patent Publication Number: US-11654293-B2

Title: Laser assisted wound healing protocol and system

Description:
CROSS REFERENCES 
     This application is an International Application claiming priority to U.S. patent application Ser. No. 15/811,651 filed Nov. 13, 2017, which is a Continuation-In-Part Application claiming the benefit of priority from U.S. patent application Ser. No. 15/348,793 filed Nov. 10, 2016, the entire contents of which are herein incorporated by reference. 
    
    
     FIELD OF THE INVENTION 
     The present invention relates generally to a method of treating gum diseases using a diode laser which produces a beam of light having a wavelength in the visible portion of the electromagnetic spectrum (400 nm-700 nm). Optionally, the laser light utilizes green wavelength range (520-570 nm) at a laser power 0.001 W to 5 W to treat wounds. It is also contemplated that described is a method of treating diseased soft or hard tissue using a diode laser. Optionally, the laser light utilizes the IR wavelength range (700 nm-1400 nm) at a laser power of 0.001 W to 5 W to treat wounds. Optionally, an LED light utilizes the IR wavelength range to treat wounds. 
     BACKGROUND OF THE INVENTION 
     Laser Assisted Periodontium and Osseous Regeneration (LAPOR) is a protocol which is laser assisted with the use of a substrate such as but not limited the LAPOR periodontal solution, the LAPOR periodontal gel and the LAPOR substrate and thus causes an increase in cell attachment of epithelial cells, gingival fibroblasts, PDL fibroblasts and adhesion of osteogenic cells. Enhanced cell migration and proliferation appears to lead to accelerated wound fill rates in vitro using PDL fibroblasts, gingival fibroblasts and osteoblast-like cells. 
     A substrate such as the LAPOR periodontal solution, the LAPOR periodontal gel and the LAPOR substrate, used in the LAPOR protocol, stimulates total protein synthesis and the synthesis of specific extracellular matrix molecules. Studies that evaluate the bone remodeling regulation system indicate that proteins influence this regulation system, thus indicating an indirect involvement in the bone remodeling process. When used in conjunction with a specially formulated periodontal and wound healing substrate or combination of substrates, and LAPOR gel root conditioner in certain cases, LAPOR has shown to stimulate total tissue and bone synthesis, increase gingival attachment, gingival height, bone density, bone height thereby showing accelerated wound fill rates in vivo. 
     The laser or LED used produces a beam of light having a wavelength in the visible portion of the electromagnetic spectrum (400 nm-700 nm). Optionally, a beam of light having a wavelength in the green wavelength range (520-570 nm) at a laser power of 0.5 to 1.2 W is used in the LAPOR protocol. It has been shown by the LAPOR protocol to biostimulate the healing and regenerative processes of the periodontium, including the biostimulation of new bone and its supporting elements. The diode laser used in the LAPOR protocol, biostimulates the healing response of the periodontium, and biostimulates the soft or hard tissue regeneration of the periodontium, and prevents long junctional epithelium from migrating downwards into the sulcus (a biomechanical aspect of tissue healing), thereby preserving the tissue height. A diode laser used in the LAPOR protocol helps a substrate such as but not limited to compounds and proteins to stimulate total protein synthesis and the synthesis of extracellular matrix molecules. 
     Alternatively, the LAPOR protocol may use a beam of light having a wavelength in the green wavelength range (520-570 nm), red wavelength range (620-750 nm), or yellow wavelength range (570-590 nm) having an alternative wattage of 0.001 W to 5 W, preferably 0.002 W to 4 W, more preferably 0.003 W to 4 W, and most preferably 0.005 W to 2 W. The diode laser used helps the substrate(s) stimulate total tissue and bone synthesis by biostimulating the healing response via soft/hard tissue regeneration of a wound and soft/hard tissue regeneration of the wound&#39;s supporting elements. 
     It is further contemplated that the invention may be used to treat soft and/or hard tissue damage in wounds, i.e. Laser Assisted Tissue and Osseous Regeneration (LATOR) using a LATOR solution, and/or LATOR gel and/or a LATOR substrate or a combination of substrates to enhance cell migration and proliferation leading to accelerated wound fill rates. The protocol is used in conjunction with a choice or any combination of six specially formulated tissue and wound healing substrates and a gel conditioner in certain cases to stimulate total tissue and bone synthesis, increase tissue attachment, tissue height, bone density and bone height thereby showing accelerated would fill rates, showing a mechanism of action as in the LAPOR protocol. 
     SUMMARY OF THE INVENTION 
     In an exemplary embodiment of the present invention, there is disclosed a method of treating wounds, including general wounds, gum disease and gingival tissues post scaling/root planning, using a diode laser which generates a beam of light having a wavelength in the visible portion of the electromagnetic spectrum (400 nm-700 nm). Optionally, a beam of light having a wavelength in the green range (520-570 nm) at a laser power of 0.5 to 1.2 watts is used to decontaminate the wound or gum tissue and to biostimulate healing while regenerating the wound or periodontium (including cementum of the root surface and/or tissues surrounding an implant), thus preventing long junctional epithelium from migrating downwards into the sulcus and thereby preserving the tissue height. Alternatively, a beam of light having a wavelength in the green wavelength range (520-570 nm), red wavelength range (620-750 nm), or yellow wavelength range (570-590 nm) having an alternative wattage of 0.001 W to 5 W may be used to biostimulate healing and regenerate the wound site, its tissue and bone. In a preferred embodiment, the wattage is in the range of 0.002 W to 4 W, more preferred in the range of 0.003 to 3 W, and most preferred in the range of 0.005 W to 2 W. A laser or LED also biostimulates the healing and regenerative response induced by a substrate, i.e. the LAPOR periodontal and wound healing solution, the LAPOR periodontal gel and the LAPOR periodontal and wound healing substrates, the method comprising: 1) placing the laser inside the sulcus; 2) penetrating the entire sulcus by moving the laser light intermittently vertically and horizontally throughout the sulcus; and 3) placing the substrate in the sulcus prior to blood clot formation. In a preferred embodiment, the LATOR protocol may use a laser per the above parameters to treat general wound sites. Optionally, the laser light utilizes the IR wavelength range (700 nm-1400 nm) at a laser power or average power of 0.001 W to 5 W to treat wounds. 
     In an alternative embodiment, the LAPOR or LATOR protocol may use an LED light to biostimulate healing and to regenerate periodontium or general wound tissue. The LED light is used at 10 W or, preferably, 9 W or lower on wounds to assist in new cell organization and hence tissue regeneration. Optionally, an LED light utilizes the IR wavelength range to treat wounds. Optionally, the laser light utilizes the IR wavelength range (700 nm-1400 nm) at a laser power of 0.001 W to 5 W to treat wounds. 
     In an alternative embodiment, the LAPOR and LATOR protocols may use a radiofrequency (RF) wave to decrease pain or decontaminate the gum tissue and biostimulate healing while regenerating the periodontium and wound. The RF beam is used at 10 W or, preferably, 9 W or lower on wounds to assist in new cell organization and hence soft and/or hard tissue regeneration. A carrier wave (sine wave) transports a non-sinusoidal waveform or waveforms to the treatment location. The carrier wave frequency may be in the range of 0.1 MHz to 20 MHz while the non-sinusoidal waveform may be in the range of 0.5 to 40 KHz or alternatively 0.5-24 GHz. In a preferred embodiment, the carrier wave frequency is in the range of 0.2 MHz to 10 MHz, preferably 0.3 MHz to 5 MHz. Optionally the 0.001 W to 10 W or 9 W range, preferably a 0.001 W to 3 W range, is utilized in the hertz range of 40 Hz to 24 GHz. In a further alternative embodiment, the RF wave is a single sine wave. In a further alternative embodiment, the RF wave is more than one sine wave wherein the more than one demonstrates a harmonics pattern. In a preferred embodiment, the LATOR protocol may use an RF wave per the above parameters to treat general wound sites. Optionally, the non-sinusoidal waveform/s may be in the range of the above parameters in the absence of a carrier wave. 
     In another embodiment of the present invention, there is disclosed a root/bone/cartilage gel conditioner comprised of EDTA 15%, calcium gluconate 20%, methylparaben, propylparaben, Ethanolamine as a buffering agent, carboxymethylcellulose, and green food coloring and sterile water. 
     In still another embodiment of the present invention, there is disclosed a first substrate comprised of: a combination of mono or disodium phosphate and sodium hydroxide in solution with a sodium content of 11 mg/100 g; 60% water; 9% Lysine; 9% Proline; 9% all other essential amino acids wherein the amino acids are chosen from the group consisting of Isoleucine, Leucine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, Histadine, Asparagine and Selenocysteine; 2% of all other non-essential amino acids wherein the amino acids are chosen from the group consisting of Alanine, Arginine, Aspartate, Cysteine, Glutamate, Glutamine, Glycine, Serine, Tyrosine and Pyrrolsine; 6.9% free bases wherein the free bases are chosen from the group consisting of adenosine, uridine, guanosine, iridin and cytidine; 2% phosphates wherein the phosphates are chosen from the group consisting of ADP, ATP and acetycholine; and 1% benzoic acid. 
     In still another embodiment of the present invention, there is disclosed a second substrate comprised of: tricalcium phosphate wherein the tricalcium phosphate is precipitated with calcium hydroxide/Claw oil; and hydroxyapatite crystals Optionally, the second substrate is comprised of tricalcium phosphate wherein the tricalcium phosphate is prepared with hydroxyapatite crystals, wherein the second substrate is comprised of dense or porous tricalcium phosphate comprising of one size or variety of sizes of crystals: 4-50 μm, 50-150 μm, 100-300 μm, 500-1000 μm, 1-3 mm, and 3-6 mm. 
     In yet another embodiment of the present invention, there is disclosed a third substrate comprised of: 5.1% hyaluronic acid; 8% fatty acids wherein the fatty acids are chosen from the group consisting of Linoleic acid (LA), alpha-linolenic acid (ALA), 4.4% sugars wherein the sugars are chosen from the group consisting of mannose, galactose, N-acetylglactosamine, N-acetylglucosamine, N-acetylneuraminic acid, fucose (L configuration minus a carboxyl group at the 6 position), and xylose; 2.2% mixture of glucose and fucose (L configuration minus a carboxyl group at the 6 position); 3% lipids wherein the lipids are chosen from the group consisting of vitamin A, vitamin D2, D3, vitamin E, vitamin K1, K2, vitamin B12 (methylcobalamin, hydroxocobalamin), cholesterol, and diaglycerol; 2.7% vitamins wherein the vitamins are chosen from the group consisting of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B9, vitamin C and pantothenic acid; 4.5% electrolyte sources: wherein the electrolyte sources are chosen from the group consisting of Calcium Chloride, Choline Chloride, Magnesium Sulfate, Potassium Chloride, Potassium Phosphate (monobasic), Sodium Bicarbonate, Sodium Chloride, and Sodium Iodide; 6% metals wherein the metals are chosen from the group consisting of Ag nanoparticles and Au nanoparticles; 3.9% ionic metals wherein the ionic metals are chosen from the group consisting of copper, zinc, selenium, iron, manganese, cobalt, chromium, boron, and molybdenum; and 4% other ionic metals wherein the other ionic metals are chosen from the group consisting of boron, silicon, nickel and vanadium. 
     In another embodiment of the present invention, there is disclosed a fourth substrate comprised of carbomer, potassium chloride, chloride, sodium, potassium, manganese, calcium tri-phosphate, sulfate, carbonate, snail serum, snail secretion filtrate, HA, Au, Ag, Cu, Fe, Pt, collagen, glyceine HCl and fucose. 
     In another embodiment of the present invention, there is disclosed as fifth substrate comprised of a dense or porous tricalcium phosphate in a variety of crystal sizes, and/or collagen limed and/or collagen unlimed. 
     In another embodiment of the present invention, there is disclosed a sixth substrate comprised of a dense or porous tricalcium phosphate in a variety of crystal sizes, and/or collagen limed and/or collagen unlimed and/or HCl and/or NaCl, and/or nanoparticles wherein the nanoparticles are chosen from the group consisting of copper, Au, Ag, iron, Fe 3 O 4 , and platinum or any combination thereof and/or compounds wherein the compounds are chosen from the group consisting of CuCl 3 , CuCl 2 , CuCl, FeCl 3 , FeCl 2 , AuCl, AuCl 2 , AuCl 3 , AgCl, AgCl 2  or any combination thereof, and/or Hyaluronic Acid and/or dense or porous hydroxyapatite in a variety of crystal sizes. 
     The more important features of the invention have thus been outlined in order that the more detailed description that follows may be better understood and in order that the present contribution to the art may better be appreciated. Additional features of the invention will be described hereinafter and will form the subject matter of the claims that follow. 
     Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not limited in its application to the details of construction and the arrangements of the components set forth in the following description or illustrated in the drawings. The invention is capable of other embodiments and of being practiced and carried out in various ways. Also, it is to be understood that the phraseology and terminology employed herein are for the purpose of description and should not be regarded as limiting. 
     As such, those skilled in the art will appreciate that the conception, upon which this disclosure is based, may readily be utilized as a basis for the designing of other structures, methods and systems for carrying out the several purposes of the present invention. It is important, therefore, that the claims be regarded as including such equivalent constructions insofar as they do not depart from the spirit and scope of the present invention. 
     The foregoing has outlined, rather broadly, the preferred feature of the present invention so that those skilled in the art may better understand the detailed description of the invention that follows. Additional features of the invention will be described hereinafter that form the subject of the claims of the invention. Those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiment as a basis for designing or modifying other structures for carrying out the same purposes of the present invention and that such other structures do not depart from the spirit and scope of the invention in its broadest form. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       Other aspects, features, and advantages of the present invention will become more fully apparent from the following detailed description, the appended claim, and the accompanying drawings in which similar elements are given similar reference numerals. 
         FIG.  1    shows an X-Ray view of a patient&#39;s teeth before treatment with a diode laser before a substrate has been applied. 
         FIG.  2 - 7    show X-Ray views of the lower teeth of  FIG.  1    after treatment with a diode laser after treatment with a substrate. 
         FIG.  8    shows an X-Ray view of the upper teeth before treatment with a diode laser after treatment with a substrate. 
         FIG.  9    shows an X-ray view of the upper teeth of  FIG.  8    after treatment with a diode laser after treatment with a substrate. 
         FIG.  10    shows a flow diagram of a method of using a diode laser to treat gum disease in accordance with the principles of the invention. 
         FIG.  11    shows bone density measurements for tooth 15 of a patient at 12 loci on the tooth following treatment with a diode laser and a substrate over time. 
         FIG.  12    shows bone density measurements for tooth 28 of a patient at 17 loci on the tooth following treatment with a diode laser and a substrate over time. 
         FIG.  13    shows bone density measurements for tooth 2, tooth 3 and tooth 15 of a patient at 3 loci per tooth following treatment with a diode laser and a substrate over time. 
         FIGS.  14   a  and  14   b    show X-rays of tooth 15 of a patient from which measurements shown in  FIG.  11    were collected. (a) shows tooth 15 before treatment. (b) shows tooth 15 at the October 2011 measurement following three treatments. 
         FIGS.  15   a  and  15   b    show X-rays of tooth 28 of a patient from which measurements shown in  FIG.  12    were collected. (a) shows tooth 28 before treatment. (b) shows tooth 28 at the January 2011 measurement following four treatments. 
         FIGS.  16   a  and  16   b    show a panoramic X-ray of tooth 2, tooth 3 and tooth 15 of a patient from which measurements shown in  FIG.  13    were collected. (a) shows the teeth before treatment. (b) shows the teeth at the July 2011 measurement. 
         FIG.  17   a - 17   f    show various views of a first embodiment of a diode laser with RF of the present invention. (a) shows a right side view. (b) shows a back side view. (c) shows a left side view. (d) shows a front side view. (e) shows a top view. (f) shows a bottom view. 
         FIG.  18   a - 18   c    show an exploded view of the diode laser of  FIG.  17   . (a) shows an exploded view. (b) shows an assembled view. (c) shows a close-up of the laser housing. 
         FIG.  19   a - 19   g    shows various views of a second embodiment of the RF device without diode laser of the present invention. (a) shows a top perspective view. (b) shows a back view. (c) shows a left side view. (d) shows a top view. (e) shows a front perspective view. (f) shows a right side view. (g) shows a bottom view. 
         FIG.  20   a - 20   d    shows views of the RF device with and without diode laser of  FIG.  19   . (a) shows an exploded view of RF device with laser. (b) shows a close-up of the handpiece with laser relative to housing. (c) shows an exploded view of RF device without laser. (d) shows detailed view of housing for RF device without laser. 
         FIG.  21   a - 21   f    show various views of a third embodiment of the RF device of the present invention, with and without the laser. (a) shows a side perspective view. (b) shows an exploded view of the RF device with laser. (c) shows a close-up view of the laser relative to the sub-housing. (d) shows a side perspective view. (e) shows an exploded view of the RF device without laser. (f) shows a detailed view of the sub-housing within the RF device without laser. 
         FIG.  22   a - 22   h    shows fiber optic handpiece and tip embodiments of the present invention, along with the power device and battery embodiments for the handpieces. (a) shows an assembled view. (b) shows an exploded view. (c) shows a flat tip. (d) shows a glass dispersion tip. (e) shows an assembled view of the power device. (f) shows an exploded view of the power device. (g) shows an assembled view of the battery pack. (h) shows an exploded view of the battery pack. 
         FIG.  23   a - g    shows flow charts of the protocol embodiments for using an RF device of the present invention, along with placement instrument embodiments for applying the preferred substrates of the present invention. (a) flow chart of the protocol along with certain parameters. (b) a preferred flow chart with preferred parameters. (c)-(e) oral surgery placement instruments. (f)-(g) periodontal placement instruments. 
         FIG.  24    shows chin profile measurements before and after treatment. 
         FIG.  25    shows toe crease length measurements before and after treatment. 
         FIG.  26   a - 26   c    show gingival wound healing and tissue regeneration measurements before and after treatment. (a) shows wounds before treatment. (b) shows tissue regeneration after treatment. (c) shows tissue height measurements before and after treatment. 
         FIG.  27    shows hand crease length measurements before and after treatment. 
         FIG.  28    shows wound new skin growth measurements before and after treatment. 
         FIG.  29    shows (anal) scar width reduction measurements before and after treatment. 
         FIG.  30    shows (anal) scar length reduction measurements before and after treatment. 
         FIG.  31    shows swallowing strength measurements before and after treatment. 
         FIG.  32    shows breast firmness measurements before and after treatment. 
         FIG.  33   a - 33   e    show various views of an RF hand piece of the present invention. (a) shows a top view. (b) shows a side view. (c) shows a perspective view of the RF tips. (d) shows an exploded side perspective view. (e) shows an alternative side perspective view. 
         FIG.  34   a - 34   f    show various views of a fourth alternative diode laser of the present invention. (a) shows a right side view. (b) shows a front view. (c) shows a left side view. (d) shows a left side perspective view. (e) shows a top view. (f) shows a right side perspective view. 
         FIG.  35    show various views of a laser power source for the fiber optic hand piece and interchangeable tips. 
         FIG.  36   a - 36   f    shows various views of a portable RF transmitter of the present invention. (a) shows a top view. (b) shows a front view. (c) shows a bottom view. (d) shows a left side view. (e) shows a left front perspective view. (f) shows a right front perspective view. 
         FIG.  37    shows epithelial wound regeneration before and after treatment. 
         FIG.  38    shows cancaeal tendon wound regeneration before and after treatment. 
         FIG.  39    shows ankle epithelial wound regeneration before and after treatment. 
         FIG.  40    shows ankle wound size reduction before and after treatment. 
         FIG.  41    shows oral cavity wound regeneration before and after treatment. 
         FIG.  42   a - b    shows (a) vein wound regeneration before and after treatment and (b) a flashlight style infrared laser. 
         FIG.  43   a - b    shows increases in tissue regeneration in (a) epithelial tissue and (b) oral cavity wound. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Definitions 
     As used herein, the term “gum disease” means periodontal disease which can lead to tooth loss and/or other health problems. Examples of periodontal disease include gingivitis, aggressive periodontitis, chronic periodontitis, periodontitis as a manifestation of systemic diseases, and necrotizing periodontal disease. 
     As used herein, the term “tissue disease” means soft or hard tissue disease of acute or chronic nature which can lead to other health problems such as amputation of limbs. 
     As used herein, the term “patient” means any individual suffering from a disease of the gums or tissue and in need of treatment for said gum or tissue disease. 
     As used herein, the term “locus” means an exact point of measurement within the sulcus or the immediate surrounding area. 
     As used herein, the term “substrate mixture” means the mixture of the first substrate and/or the second substrate and/or the third substrate and/or the fourth substrate and/or the fifth substrate and/or sixth substrate disclosed herein for treatment of gum disease and/or tissue disease and/or wounds. 
     As used herein, the term “substrate” means any stand alone substrate of the substrates disclosed herein for treatment of gum disease and/or tissue disease and/or wounds. 
     As used herein, the term “bone regeneration” means increasing the density of calcium at specific loci in or around the sulcus or the immediate surrounding area. 
     As used herein, the term “calcium density” means the measurement of calcium mass around a given loci. 
     As used herein the term “wound” means any area that has lost any original tissue or bone or any other structure not named that lost a healthy non-wounded, undamaged and unaged form. 
     As used herein, the terms “power” and “average power” are considered synonymous, with “power” referring to continuous power applied to lasers, LEDs or RF devices while “average power” refers to pulsed power applied to lasers, LEDs or RF devices of the present invention. 
     As used herein, the terms “laser” or “LED” or “RF” mean types of energy and may be used with or without substrate. 
     The LAPOR protocol can be used in the treatment of gum disease and wounds by combining the most effective methods of treatment with the use of a special laser. Approximately 66% of the United States population has some form of gum disease. But many avoid seeking treatment because of the discomfort that often results from gum surgery. LAPOR provides a new choice. The LAPOR protocol is a treatment that is more effective than traditional periodontal surgery, and it is much more beneficial to the patient both in the short and long term. The LATOR protocol can similarly be used for treatment of soft or hard tissue disease and wounds. 
     The LAPOR protocol takes only about an hour and only two short follow-up visits. Patients enjoy no downtime with recovery taking only 24 hours. This makes immediate return to work both possible and comfortable. 
     After having the LAPOR protocol performed, no resulting gum recession occurs when compared to that which most often follows normal periodontal surgery. This, combined with new cementum formation on the roots, bone formation in previous defects or around failing implants, periodontal ligament formation. After having the LATOR protocol performed in a chronic wound, no resulting subsequent wound fibrosis is found compared to that which most often follows normal treatment, new soft and/or hard tissue formation occurs multi directionally and the wound closes without grafting. 
     The LAPOR and LATOR protocols of the present invention can be used to heal wound sites by combining or using separately the most effective methods of treatment with the laser, LED, radiofrequency energy and substrates. In a preferred embodiment, the RF energy waves may be up to 10 W, with most preferably being only as high as 9 W. The carrier wave frequency may be in the range of 0.1 MHz to 20 MHz while the non-sinusoidal waveform/s may be in the range of 0.5 to 40 KHz or from 0.5 to 24 GHz. In a preferred embodiment, the carrier wave frequency is in the range of 0.2 MHz to 10 MHz, preferably 0.3 MHz to 5 MHz. Optionally a 0.001 W to 10 W range RF energy, preferably a 0.001 W to 3 W range, is utilized in the hertz range of 40 Hz to 24 GHz. In a further alternative embodiment, the RF wave is more than one square wave wherein the more than one may demonstrate a pattern. In a further alternative embodiment, the RF wave is more than one sine wave wherein the more than one demonstrates a harmonics pattern. 
     The special type of laser used in the LAPOR protocol and the LATOR protocol is the diode, a semiconductor coherent light beam. The laser or LED light used has a wavelength in the visible portion of the electromagnetic spectrum, between 400 nm-700 nm wavelength. Optionally, the green range (520-570 nm) of the visible spectrum is utilized at a laser power or average power of 0.5 to 1.2 watts, which disinfects the site, leaving the gum tissue bacteria free, and biostimulates healing; in conjunction with treatment with a substrate, the laser biostimulates regeneration of the periodontium. Traditional periodontal therapy removes tissue height of a tooth or implant to reduce the pocket depths. The LAPOR protocol is a regenerative procedure. The patient does not lose tissue volume. Tissue volume is increased and bone is regenerated. For general tissue disease, the laser biostimulates regeneration of tissue where traditional therapy removes tissue height to reduce the disease. Optionally, the laser light utilizes the IR wavelength range (700 nm-1400 nm) at a laser power or average power of 0.001 W to 5 W to treat wounds. Optionally, each LED light is used at 10 W or, preferably, 9 W or lower. 
     The use of the diode laser in conjunction with routine scaling and root planning is more effective than scaling and root planning alone. It enhances the speed and extent of the patients gingival healing and postoperative comfort. This is accomplished through laser bacterial reduction and biostimulation with a laser light having a wavelength in the visible portion of the electromagnetic spectrum, between 400 nm-700 nm wavelength. Optionally, the green range (520-570 nm) of the visible spectrum is utilized at a laser power or average power of 0.5 to 1.2 watts. Alternatively, the laser power wattage may be in the range of 0.001 W to 5 W, preferably 0.002 W to 4 W, more preferably 0.003 W to 3 W, and most preferably 0.005 W to 2 W. 
     Referring to  FIG.  10   , there is disclosed a method  10  of using a diode laser which produces a beam of light, used intermittently, having a wavelength in the visible portion of the electromagnetic spectrum, between 400 nm-700 nm wavelength. Optionally, the green range (520-570 nm) of the visible spectrum is utilized at a laser power of 0.5 to 1.2 watts to treat gum disease. Starting at block  12 , a perio probe determines the degree of excessive pocket depth and thus helps the dentist better identify diseased soft and hard tissue and areas of bacterial infection. The dentist removes calculus from the root or implant surface using an ultrasonic scaler and hand instruments, block  14 . This action by the dentist helps stimulate a healing response in the sulcus by opening the capillaries upon scaling. Going to block  18 , the laser tip is placed inside the sulcus and a continuous light beam with intermittent stops for tissue temperature control is allowed to penetrate the entire sulcus by moving the tip vertically and horizontally throughout the sulcus. The laser tip is cut at a 45 degree angle during the first pass. The laser is cut at the opposite 45 degree angle during the second pass. This allows for the laser beam to penetrate the existing periodontium to decontaminate the tissue, as the heat of the targeted laser light kills the bacteria. This also allows for biostimulation of the sulcular contents. At block  20 , the dentist scales the sulcular area and root/implant surfaces once again to induce a healing response through renewed blood flow. Going to block  22 , at least one substrate, such as but not limited to matrix proteins, is then placed in the sulcus of the tooth prior to the blood clot forming and at block  24 , a blood clot is carefully allowed to form by gently helping patient keep their mouth open for 5 minutes, to keep the substrate intact. Optionally, the laser light utilizes the IR wavelength range (700 nm-1400 nm) at a laser power of 0.001 W to 5 W to treat wounds. 
     Alternatively, the laser tip is a specially designed tip that disperses light energy throughout the wounded sulcus which allows the laser beam to penetrate the existing tissues to decontaminate the tissue, as the heat of the targeted laser light kills the bacteria and as a result block  20  may be eliminated going directly to block  22   
     The LAPOR protocol is much less invasive than traditional surgery and offers advantages and benefits over its counterpart. Recovery time is much faster because most, if not all, damage to healthy tissue is avoided through the use of more advanced technology. Because the LAPOR protocol leaves healthy tissue intact, the height of the gums themselves increases around the teeth and is better preserved. The LAPOR protocol prevents long junctional epithelium from migrating downwards into the sulcus, thus preserving the tissue height and allowing for the regeneration of the periodontium. 
     Referring to  FIG.  17   a - 17   f   , shown are various angles of the first embodiment of a device  100  for use in conjunction with the substrates and methods of the present invention.  FIGS.  18   a  and  18   b    show perspective views of the device  100  in the first embodiment. Specifically,  FIG.  18   a    illustrates an exploded view of the device  100  comprised of cord  101  integrally connected to handle  109 , handle  109  further connected to heat sink  102 . Housing  103  securely connects to heat sink  102  thereby creating a cavity between the housing  103  and heat sink  102 . Laser  104  is positioned within the cavity between housing  103  and heat sink  102 . Male connectors  105  connect RF source  108  to housing  103  wherein threaded inserts  106  cover the connection therebetween. Cap  107  is positioned over housing  103  and secures to handle  109 .  FIG.  18   c    shows a detailed view of heat sink  102 , laser  104 , housing  103  and male connectors  105  in relation to each other. In a preferred embodiment, the device  100  may have a plurality of RF sources  108  wherein a plurality is defined as at least two tips (i.e. dipole). Housing  103  is capable of movement such that RF source  108  may be adjusted 45° up or down relative to the x-axis for ease of use depending upon the location of the wound receiving treatment. 
     The laser energy may have wavelength in the green wavelength range (520-570 nm), red wavelength range (620-750 nm), or yellow wavelength range (570-590 nm) having a wattage of 0.001 W to 5 W. In a preferred embodiment, laser energy has a wattage of 0.001 W to 5 W. The wattage is in the range of 0.001 W to 4 W, more preferred in the range of 0.003 to 3 W, and most preferred in the range of 0.005 W to 2 W. The RF energy may have a power of 9 watts or lower. The carrier wave frequency may be in the range of 0.1 MHz to 20 MHz while the non-sinusoidal waveform may be in the range of 0.5 to 40 KHz or from 0 to 24 GHz. In a preferred embodiment, the carrier wave frequency is in the range of 0.2 MHz to 10 MHz, preferably 0.3 MHz to 5 MHz. Optionally a 0.001 W to 10 W range RF energy, preferably a 0.001 W to 3 W range, is utilized in the hertz range of 40 Hz to 24 GHz. In a further alternative embodiment, the RF wave is more than one sine wave wherein the more than one demonstrates a harmonics pattern. Optionally, the non-sinusoidal waveform may be single or multiple and in the range of the above parameters in the absence of a carrier wave. 
     Referring to  FIG.  19   a - 19   g   , shown are various angles of a second embodiment of a device  200  for use in conjunction with the substrates and methods of the present invention.  FIG.  20   a    shows a perspective view of the device  200  of the second embodiment. Specifically,  FIG.  20   a    illustrates an exploded view of the device  200  comprised of wire grommet  201  integrally connected to handle  209 , handle  209  further comprised of heat sink  202 . Housing  203  securely connects to heat sink  202  thereby creating a cavity between the housing  203  and heat sink  202 . Laser  204  is positioned within the cavity between housing  203  and heat sink  202 . Male connectors  205  connect RF source  208  to housing  203  wherein threaded inserts  206  cover the connection there between.  FIG.  20   b    shows a detailed view of laser  204  and housing  203  in relation to each other. 
     Referring to  FIG.  21   a - 21   c   , shown is a third embodiment of a device  300  for use in conjunction with the substrates and methods of the present invention.  FIG.  21   a    shows a perspective view of the device  300  of the second embodiment. Specifically,  FIG.  21   b    illustrates an exploded view of the device  300  having an external housing  307  and further comprised of wire grommet  301  integrally connected to sub-housing  303 , sub-housing  303  further comprised of heat sink  302 . Sub-housing  303  securely connects to heat sink  302  thereby creating a cavity between the sub-housing housing  303  and heat sink  302 . Laser  304  is positioned within the cavity between sub-housing housing  303  and heat sink  302 . Male connectors  305  connect RF tips  308  to sub-housing housing  303  wherein threaded inserts  306  cover the connection there between.  FIG.  21   c    shows a detailed view of laser  304  and sub-housing housing  303  in relation to each other. By way of example only, the device may have five or six tips. 
     Referring to  FIG.  21   d - 21   f   , shown is a third embodiment of a device  300  for use in conjunction with the substrates and methods of the present invention.  FIG.  21   d    shows a perspective view of the device  300  of the second embodiment. Specifically,  FIG.  21   e    illustrates an exploded view of the device  300  comprised of wire grommet  301  integrally connected to sub-housing  303 , sub-housing  303  further comprised of heat sink  302 . Sub-housing  303  securely connects to heat sink  302  thereby creating a cavity between the sub-housing  303  and heat sink  302 . RF tips  308  are positioned within the openings in close proximity to sub-housing  303 . Male connectors  305  connect RF source  308  to sub-housing  303  wherein threaded inserts  306  cover the connection there between.  FIG.  21   f    shows a detailed view sub-housing  303 . By way of example only, the device may have five or six tips. 
     Referring to  FIGS.  22   a  and  22   b   , shown is a fiber optic device  400  for use in conjunction with the substrates and methods of the present invention. Fiber optic device  400  is comprised of hand grip assembly  401  disposed between a first end and a second end. The first end is further comprised of nose insert  402  positioned between hand grip assembly  401  and removable nose assembly  404 . Bent fiber tube  405  extends from removable nose assembly  405 . The second end is further comprised of base insert  406  positioned between hand grip assembly  401  and rubber boot  407 . Extending from rubber boot  407  is sheathed fiber  408  having a SMA connector  409  at the end opposite rubber boot  407 . 
     Referring to  FIG.  22   c - 22   d   , shown are alternative embodiments of interchangeable fiber optic tips for a laser for use in conjunction with the substrates and methods of the present invention.  FIG.  22   c    shows an interchangeable fiber optic tip for a laser having a nose piece  900  and a flat tip  901 .  FIG.  22   d    shows an interchangeable fiber optic tip for a laser having a nose piece  900  and a glass dispersion tip  902 . 
     Referring to  FIGS.  22   e  and  22   f   , shown are preferred embodiments of a power device suitable for accommodating the handpieces of the present invention.  FIG.  22   e    shows a complete power device  2201 .  FIG.  22   f    shows an exploded view of the power device  2201  of  FIG.  22   e   . The components include a heatsink  2203  positioned above a heatsink base  2202 . A fiber optic laser  2204  sits above the heatsink  2203 , wherein a fiber optic attachment  2205  sits above the fiber optic laser  2204  and is exposed through the top  2207  of the device  2201 . A battery mount  2206  having device-side charging tips  2209  is positioned in order to accommodate a battery (not shown). A power switch  2208  appears through the top  2207  of the device  2201 . 
     Referring to  FIGS.  22   g  and  22   h   , shown are preferred embodiments of the battery pack for the power device for the handpieces of the present invention.  FIG.  22   g    shows a battery pack  2210 .  FIG.  22   h    shows an exploded view of the battery pack  2210 , including a base  2211  into which the battery  2212  is fitted. The battery  2212  is in contact with battery-side charging tips  2213  that abut charging housing  2215  through a top  2214  of the battery pack  2210 . 
     Referring to  FIGS.  23   a - g   , shown are flow charts and preferred instruments for placement of substrate for given procedures.  FIGS.  22   a  and  22   b    detail specific protocols and certain parameters for operating the devices of the present invention.  FIGS.  22   c - 22   e    detail certain placement instruments used in oral surgery procedures.  FIGS.  22   f  and  22   g    describe certain placement instruments for use in periodontal procedures. 
     Firstly, the conditioner is applied to the root or bone surface. The root conditioner comprises the following at Table 1: 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 1 
               
               
                   
                   
               
               
                   
                 Component 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 EDTA 
                 20-25 
                 g. 
               
               
                   
                 Calcium gluconate 
                 10-20 
                 g. 
               
               
                   
                 Methylparaben 
                 .1-.9 
                 g. 
               
               
                   
                 Propylparaben 
                 .01-.1 
                 g. 
               
               
                   
                 Ethanolamine 
                 2-8 
                 mls. 
               
               
                   
                 Carboxymethylcellulose 
                 2-10 
                 g. 
               
               
                   
                 Green food coloring 
                 1-2 
                 drops 
               
               
                   
                 Sterile water 
                 100 
                 mls. 
               
               
                   
                   
               
            
           
         
       
     
     The conditioner is optionally rinsed out prior to application of additional substrates or laser light. Alternatively, the conditioner is left on the root or bone surface with the laser light being applied prior to application of any substrate. In an alternative embodiment, the conditioner is left in with only one substrate applied prior to application of the laser light. Optionally, the conditioner is left in the sulcus and substrate is added prior to any application of laser light. 
     The placement of the substrate into the sulcus containing luminesced blood enables the luminesced blood to coagulate upon the substrate. 
     Optionally, the liquid substrate or substrate 1 is comprised of the following, per 1 L of solution, at Table 2: 
     
       
         
           
               
               
             
               
                   
                 TABLE 2 
               
               
                   
                   
               
               
                   
                 % 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Essential 
                   
               
               
                   
                 Amino Acids 
               
               
                   
                 Isoleucine 
                 1.125 
               
               
                   
                 Leucine 
                 1.125 
               
               
                   
                 Methionine 
                 1.125 
               
               
                   
                 Phenylalanine 
                 1.125 
               
               
                   
                 Threonine 
                 1.125 
               
               
                   
                 Tryptophan 
                 1.125 
               
               
                   
                 Valine 
                 1.125 
               
               
                   
                 Histidine 
                 1.125 
               
               
                   
                 Lysine 
                 9 
               
               
                   
                 Non-Essential 
               
               
                   
                 Amino Acids 
               
               
                   
                 Alanine 
                 0.25 
               
               
                   
                 Arginine 
                 0.25 
               
               
                   
                 Aspartate 
                 0.75 
               
               
                   
                 Glutamate 
                 0.25 
               
               
                   
                 Glycine 
                 0.25 
               
               
                   
                 Serine 
                 0.25 
               
               
                   
                 Proline 
                 9 
               
               
                   
                 Phosphates 
               
               
                   
                 ADP 
                 0.667 
               
               
                   
                 ATP 
                 0.667 
               
               
                   
                 Acetylcholine 
                 0.667 
               
               
                   
                 Free Bases 
               
               
                   
                 Adenosine 
                 1.725 
               
               
                   
                 Uridine 
                 1.725 
               
               
                   
                 Guanosine 
                 1.725 
               
               
                   
                 Cytidine 
                 1.725 
               
               
                   
                 Benzoic Acid 
                 1 
               
               
                   
                 Sodium 
                 1.1 
               
               
                   
                 Chloride 
               
               
                   
                 Sterile water 
                 60 
               
               
                   
                 Total: 
                 100 
               
               
                   
                   
               
            
           
         
       
     
     Optionally, the total sterile water component is adjusted 20% up or down, depending on the desired viscosity to be achieved. 
     Optionally, the sterile water component also contains some amount of Hyaluronic Acid. 
     In an alternative embodiment, the liquid substrate or substrate 1 is comprised of the following, at Table 3: 
     
       
         
           
               
               
             
               
                   
                 TABLE 3 
               
               
                   
                   
               
               
                   
                 Grams 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Essential 
                   
               
               
                   
                 Amino Acids 
               
               
                   
                 Isoleucine 
                 11.25 
               
               
                   
                 Leucine 
                 11.25 
               
               
                   
                 Methionine 
                 11.25 
               
               
                   
                 Phenylalanine 
                 11.25 
               
               
                   
                 Threonine 
                 11.25 
               
               
                   
                 Tryptophan 
                 11.25 
               
               
                   
                 Valine 
                 11.25 
               
               
                   
                 Histidine 
                 11.25 
               
               
                   
                 Lysine 
                 90 
               
               
                   
                 Non-Essential 
               
               
                   
                 Amino Acids 
               
               
                   
                 Alanine 
                 2.5 
               
               
                   
                 Arginine 
                 2.5 
               
               
                   
                 Aspartate 
                 7.5 
               
               
                   
                 Glutamate 
                 2.5 
               
               
                   
                 Glycine 
                 2.5 
               
               
                   
                 Serine 
                 2.5 
               
               
                   
                 Proline 
                 90 
               
               
                   
                 Phosphates 
               
               
                   
                 ADP 
                 7-8 
               
               
                   
                 ATP 
                 7-8 
               
               
                   
                 Acetylcholine 
                 6-7 
               
               
                   
                 Free Bases 
               
               
                   
                 Adenosine 
                 13-14 
               
               
                   
                 Uridine 
                 13-14 
               
               
                   
                 Guanosine 
                 13-14 
               
               
                   
                 Cytidine 
                 13-14 
               
               
                   
                 Iridine 
                 13-14 
               
               
                   
                 Benzoic Acid 
                 20 
               
               
                   
                 Sodium 
                 .1-.9 
               
               
                   
                 Chloride 
               
               
                   
                 Sterile water 
                   .9-1.2 L 
               
               
                   
                   
               
            
           
         
       
     
     In an alternative embodiment, an additional substrate may be applied, the additional substrate, substrate 2, comprised of dense or porous tricalcium phosphate comprising of one size or variety of sizes of crystals: 4-50 μm, 50-150 μm, 100-300 μm, 500-1000 μm, 1-3 mm, and 3-6 mm and hydroxyapatite crystals. 
     In an alternative embodiment, an additional substrate may be applied, the additional substrate, substrate 3, is comprised of the following at Table 4: 
     
       
         
           
               
               
             
               
                 TABLE 4 
               
               
                   
               
               
                   
                 200 mg to 5.1 g 
               
               
                 Hyaluronic acid 
                 Grams 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Fatty acids 
                   
               
               
                 Linoleic acid (LA) 
                 4 
               
               
                 Alpha-linolenic acid (ALA) 
                 4 
               
               
                   
                 8 
               
               
                 Sugars (except glucose and fucose) 
               
               
                 Mannose 
                 0.6 
               
               
                 Galactose 
                 0.6 
               
               
                 N-acetylgalactosamine 
                 0.6 
               
               
                 N-acetylglucosamine 
                 0.6 
               
               
                 N-acetylneuraminic acid 
                 0.6 
               
               
                 Fucose (L config. and no carboxyl at 6 position) 
                 0.6 
               
               
                 Xylose 
                 0.6 
               
               
                   
                 4.2 
               
               
                 Glucose 
                 1.1 
               
               
                 Fucose (L config. and no carboxyl at 6 position) 
                 1.1 
               
               
                 Lipids 
               
               
                 A 
                 0.3 
               
               
                 D2 
                 0.3 
               
               
                 D3 
                 0.3 
               
               
                 E 
                 0.3 
               
               
                 K1 
                 0.3 
               
               
                 K2 
                 0.3 
               
               
                 B12 (Methylcobalamin) 
                 0.3 
               
               
                 B12 (Nydroxocobalamin) 
                 0.3 
               
               
                 Cholesterol 
                 0.3 
               
               
                 Diaglycerol 
                 0.3 
               
               
                   
                 3.0 
               
               
                 Vitamins 
               
               
                 B1 
                 0.3 
               
               
                 B2 
                 0.3 
               
               
                 B3 
                 0.3 
               
               
                 B5 
                 0.3 
               
               
                 B6 
                 0.3 
               
               
                 B7 
                 0.3 
               
               
                 B9 
                 0.3 
               
               
                 C 
                 0.3 
               
               
                 Pantothenic acid 
                 0.3 
               
               
                   
                 2.7 
               
               
                 Electrolyte Sources 
               
               
                 Calcium chloride 
                 .5 
               
               
                 Choline Chloride 
                 .5 
               
               
                 Magnesium Sulfate 
                 .5 
               
               
                 Potassium Chloride 
                 .5 
               
               
                 Potassium Phosphate-monobasic 
                 1 
               
               
                 Sodium Bicarbonate 
                 .5 
               
               
                 Sodium Chloride 
                 .5 
               
               
                 Sodium Iodide 
                 .5 
               
               
                   
                 4.5 
               
               
                 Metals 
               
               
                 Ag nanoparticles 
                 0.3 
               
               
                 Au nanoparticles 
                 0.3 
               
               
                   
                 0.6 
               
               
                 Iconic metals 
               
               
                 Copper 
                 0.3 
               
               
                 Zinc 
                 0.3 
               
               
                 Selenium 
                 0.3 
               
               
                 Iron 
                 0.3 
               
               
                 Manganese 
                 0.3 
               
               
                 Cobalt 
                 0.3 
               
               
                 Chromium 
                 0.3 
               
               
                 Boron 
                 0.3 
               
               
                 Molybdenum 
                 0.3 
               
               
                   
                 2.7 
               
               
                 Other ionic metals 
               
               
                 Boron 
                 0.3 
               
               
                 Silicon 
                 0.3 
               
               
                 Nickel 
                 0.3 
               
               
                 Vanadium 
                 0.3 
               
               
                   
                 1.2 
               
               
                 Benzoic Acid 
                 Up to 10.1 
               
               
                 Sodium Chloride 
                 .1-.9 
               
               
                 Sterile water 
                 60-300 ml 
               
               
                   
               
            
           
         
       
     
     Optionally, the total sterile water component is adjusted 20% up or down, depending on the desired viscosity to be achieved. 
     In an alternative embodiment, an additional substrate may be applied, the additional substrate, substrate 4, is comprised of the following at Table 5: 
     
       
         
           
               
               
               
             
               
                 TABLE 5 
               
               
                   
               
               
                 Component 
                 Grams 
                 Notes 
               
               
                   
               
             
            
               
                 carbomer 
                   10-40 
                 For acute wound (see FIG. 39) use 
               
               
                   
                   
                 10-20 
               
               
                   
                   
                 For chronic wound (see FIG. 38) use 
               
               
                   
                   
                 25-40 
               
               
                 Electrolytes 
               
               
                 Potassium chloride 
                 0.5-3 
               
               
                 Chloride 
                 0.1-1 
               
               
                 Sodium 
                 0.1-1 
               
               
                 Potassium 
                 0.1-1 
               
               
                 Manganese 
                 0.1-1 
               
               
                 Calcium tri- 
                 0.5-4 
               
               
                 phosphate 
               
               
                 Sulfate 
                 0.1-1 
               
               
                 Bicarbonate 
                 0.1-1 
               
               
                 Snail serum 
                   
                 50-150 ml; for chronic wounds 
               
               
                 Snail secretion 
                 50-150 mg 
                 substrate 6 may be incorporated 
               
               
                 filtrate 
                   
                 (see FIG. 41); decreases depending 
               
               
                 HA 
                   3-6 
                 of volume of substrate 6 
               
               
                 Au 
                 0.1-1 
               
               
                 Ag 
                 0.1-1 
               
               
                 Cu 
                 0.1-1 
               
               
                 Fe 
                 0.1-1 
               
               
                 Pt 
                 0.1-1 
               
               
                 Collagen 
                   50-150 
               
               
                 fucose 
                 0.5-1 
               
               
                 Glyceine HCl 
                   0-0.4 
               
               
                   
               
            
           
         
       
     
     Metals may be increased 50% for chronic wounds (see  FIG.  40   ). 
     Optionally, the total sterile water component is adjusted 20% up or down, depending on the desired viscosity to be achieved. 
     Substrates may have different modalities of delivery, for example; liquid, gel, drops, sprays, injections or intravenous having the same ingredients, as well as sublingual, anal, foam and ointment formulations or drinkable liquids. 
     In an alternative embodiment, an additional substrate may be applied, the additional substrate 5 is comprised of the following:
         1. collagen, limed and/or   2. collagen, unlimed, and/or   3. collagen, supplemented with porous tricalcium phosphate crystals with one size or variety of sizes: 4-50 μm, 50-150 μm, 100-300 μm, 500-1000 μm, 1-3 mm and 3-6 mm. The tricalcium phosphate crystals may be dense or porous. Optionally, substrate 5 may be used in the absence of other substrates.       

     In an alternative embodiment, an additional substrate may be applied, the additional substrate 6 is comprised of the following:
         1. collagen, limed and/or   2. collagen, unlimed and/or   3. HCl and/or   4. NaCl and/or   5. Cu, Ag, Fe, Au, Pt or any combination thereof and/or   6. Collagen, supplemented with porous tricalcium phosphate crystals with one size or variety of sizes: 4-50 μm, 50-150 μm, 100-300 μm, 500-1000 μm, 1-3 mm and 3-6 mm. The tricalcium phosphate crystals may be dense or porous. Optionally, substrate 6 may be used in the absence of other substrates.   7. nanoparticles wherein the nanoparticles are chosen from the group consisting of copper, Au, Ag, iron, Fe 3 O 4 , and platinum or any combination thereof and/or   8. compounds wherein the compounds are chosen from the group consisting of CuCl 3 , CuCl 2 , CuCl, FeCl 3 , FeCl2, AuCl, AuCl 2 , AuCl 3 , AgCl, AgCl 2  or any combination thereof, and/or   9. Hyaluronic Acid and/or   10. Hydroxyapetite, crystals, dense or porous and in a variety of crystal sizes.       

     An additional substrate may be applied, the additional substrate comprised of the following: a mixture of tricalcium phosphate and hydroxyapatite crystals. The tricalcium phosphate is precipitated prepared with HCL CaOH/devil&#39;s claw oil, in a preferred embodiment. Optionally, the additional substrate includes 50% tricalcium phosphate prepared with 50% hydroxyapatite crystals. The tricalcium phosphate crystals used are granules in the following sizes: 4-50 μm, 50-150 μm, 100-300 μm, 500-1000 μm, 1-3 mm and 3-6 mm. The tricalcium phosphate crystals may be dense or porous. 
     The additional substrate may be comprised of hydroxyapatite crystals of granules containing the following sizes: 10-50 μm, 50-150 μm, 100-300 μm, 500-1000 μm, 1-3 mm and 3-6 mm. The hydroxyapatite crystals may be dense or porous. 
     In the following examples, the conditioner is applied and subsequently rinsed out. Optionally, the conditioner is left in the sulcus. 
     After the conditioner is applied, the sulcus is biostimulated with a laser light. After this occurs, the liquid substrate is applied. Optionally, the additional substrate is applied. For cavities other than oral cavities, a diluted substrate assists treatment when ingested or taken via IV is beneficial although not required. 
     In an alternative embodiment, an optional spray substrate, spray  1 , may be applied, the spray comprised of the following: Au, and/or Ag, and/or Cu, and/or Fe, and/or Pt, and sterile water. 
     In an alternative embodiment, an optional spray substrate, spray  2 , may be applied, the spray comprised of the following: Cl, Na, K, Mg, Phosphate, Sulfate, bicarbonate and sterile water. 
     The fiber optic device of the present invention is the preferable device placed inside the sulcus for treatment. Optionally more than one fiber can be in the handpiece and each fiber can be of a different wavelength and different average power. The sulcus or wound may also be treated with laser, RF or laser with RF or LED. The remaining disclosed embodiments of the device may be used in wound treatment in conjunction with the substrates depending on the wound site and severity of the wound. Substrates disclosed herein may be a form including, but not limited to, liquid, tablet, enema, gel, injection or foam. 
     Alternative RF and/or Laser Assisted Wounded Tissue Regeneration: 
     
         
         
           
             1. Optionally scale/root plane; 
             2. Optionally etch root of tooth or implant; 
             3. Rinse with saline water; 
             4. Optionally place tip of laser, LED or RF into or around the sulcular or any other wound, and turn the laser on for up to 5 seconds or more; 
             5. Repeat step 4 circumferentially vertically and horizontally around tooth or implant until the entire wound has been saturated by energy; 
             6. Place Substrate 1, and/or 2, and/or 3, and/or 4 and/or 5 and/or 6 into glass dappen dishes; 
             7. Mix the desired amount of substrate 1, and/or 2 and/or 3 and/or 4 and/or 5 and/or 6 in dappen dish; 
             8. Place the desired mixture into the sulcular wound or wound where bone/tissue loss occurred; 
             9. Wait a few seconds; 
             10. Place more of the mixture into the sulcular wound where bone/tissue damage occurred if necessary; 
             11. Wait a few seconds; 
             12. Repeat steps 8 until all defects have been filled; 
             13. Wait 1 minute; 
             14. Optionally place hand piece with its laser and/or RF tip and/or LED tip, with or without laser, in proximity to the wound, turn on and keep in position for 1 minute; 
             15. Wait 10 seconds; and 
             16. Repeat RF step 14 until entire wound has been covered with energy and without laser.
 
Alternative RF and/or Laser Assisted Wounded Tissue Repair:
 
             1. Optionally cleanse wound with saline; 
             2. Place any substrate or any combination of substrates onto or into wound; 
             3. Direct RF/laser, RF, LED, or laser energy at wound for 1 minute or longer; 
             4. Place another layer of a chosen substrate or any combination of substrates onto or into wound if necessary; 
             5. Wait 10 seconds; 
             6. Repeat steps 2-5 until wound bed is covered; and 
             7. Alternatively wait a specified period of time in between step 2-6. 
           
         
       
    
     Treatment of the oral cavity, head/neck, tongue, anal, vaginal region and the deeper or surrounding areas reached while treating these may be performed with the RF with substrate (applied substrate or drank with water), RF without substrate, RF plus laser with substrate (applied substrate or drank with water), RF plus laser without substrate and laser with substrate (applied substrate or drank with water), laser without substrate. The treatment described may be utilized throughout the gastrointestinal tract, head/neck and anus. The laser, RF or LED treatment applied to the oral cavity and surrounding structures, anal cavity and its surrounding structures, head and neck region and its surrounding structures has benefits in deeper areas of the structures. Those deeper areas of the corresponding structures are thus part of the treatment site. Surrounding structures include, but are not limited to, all bone, cartilage, muscles, tendons, nerves, blood vessels, epithelium and fascia. 
     RF and/or Laser Assisted Head and Neck Wound Tissue Repair: 
     
         
         
           
             1. Optionally drink 4 oz. diluted Substrate 1; 
             2. Wait 15 minutes; 
             3. Optionally drink 4 oz. diluted Substrate 3; 
             4. Wait 15 minutes; 
             5. Direct RF/laser, RF or laser energy at head and neck location and the surrounding structures where wound occurred; 
             6. Keep energy in place or move over desired area until desired effect achieved; and 
             7. Move on to next site until desired result achieved. 
             8. Procedure can be started at Step 1 or Step 5. 
           
         
       
    
     Surrounding structures include, but are not limited to, all bone, cartilage, muscles, tendons, nerves, blood vessels, and epithelium. 
     Head and neck includes, but is not limited to, all structures of the head and neck including esophagus and its surrounding structures, mouth including all interior mouth structures such as tongue (entire area of tongue including but not limited to anterior, posterior, dorsal, ventral, and sublingual), floor of mouth including but not limited to arterial and nerve beds, linea alba, buccal mucosa, buccal flanges, lingual flanges, nose, interior of nose (including but not limited to the epithelial lining), all muscles and other structures of the tongue and surrounding the tongue, all muscles of the eye and surrounding the eye, all arterial, venous and nerve beds of the eye and surrounding the eye. All muscles, nerves, veins, all glands, soft and hard tissue of the head and neck, and any other structure of the head and neck. 
     RF and/or Laser Assisted Vaginal Wound Repair: 
     1. Optionally drink 4 oz. diluted Substrate 1; 
     2. Wait 15 minutes; 
     3. Optionally drink 4 oz. diluted Substrate 2; 
     4. Wait 15 minutes; 
     5. Direct RF/laser, RF or laser energy at the vagina and its surrounding structures; 
     6. Keep energy in place for 10-20 minutes or until desired effect achieved; 
     7. Rotate hand piece; and 
     8. Repeat steps 5-7 until desired result achieved. 
     9. Procedure can be started at Step 1 or Step 5. 
     Surrounding structures include, but are not limited to, all bone, cartilage, muscles, tendons, nerves, blood vessels, and epithelium. 
     RF and/or Laser and/or LED Assisted Wound/Tissue Repair—Sphincter Ani Externis: 
     
         
         
           
             1. Direct RF/laser, RF or laser energy at the anus and its surrounding structures; 
             2. Keep energy in place until desired effect achieved; 
             3. Rotate hand piece if necessary 
             4. Repeat steps 1-4 until desired result achieved. 
           
         
       
    
     Surrounding structures include, but are not limited to, all bone, cartilage, muscles, tendons, nerves, blood vessels, and epithelium and any other structures of the anal cavity. 
     RF and/or Laser Assisted Wound Repair/Tissue Repair—Breast: 
     
         
         
           
             1. Optionally drink 4 oz. diluted Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5; 
             2. Wait 15 minutes; 
             3. Optionally drink 4 oz. diluted Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5; 
             4. Wait 15 minutes; 
             5. Direct RF/laser, RF or laser energy at the breast and structures related to the breast; 
             6. Keep energy in place for 10-20 minutes or until desired effect achieved; 
             7. Rotate hand piece; and 
             8. Repeat steps 5-7 until desired result achieved. 
             9. Procedure can be started at Step 1 or Step 5.
 
Related structures include, but are not limited to, all bone, cartilage, muscles, tendons, nerves, blood vessels, lymph nodes and epithelium.
 
RF and/or Laser Assisted Wound/Tissue Repair, Tongue and its Supporting Structures in the Swallowing Mechanism:
 
             1. Optionally drink 4 oz. diluted Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5; 
             2. Wait 15 minutes; 
             3. Optionally drink 4 oz. diluted Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5; 
             4. Wait 15 minutes; 
             5. Direct RF/laser, RF or laser energy at tongue and its surrounding structures; 
             6. Keep energy in place for 10-20 minutes or until desired effect achieved; 
             7. Rotate hand piece if necessary; and 
             8. Repeat steps 5-7 until desired result achieved. 
             9. Procedure can be started at Step 1 or Step 5.
 
Surrounding structures include, but are not limited to, all bone, cartilage, muscles, tendons, nerves, blood vessels, and epithelium.
 
RF and/or Laser Assisted Wound/Tissue Repair, Tongue and its Supporting Structures in the Swallowing Mechanism:
 
             1. Direct RF/laser, RF or laser energy at tongue and its surrounding structures lingually, labially, sublingually, pharyngeally or buccally; 
             2. Keep energy in place for until desired effect achieved; 
             3. Rotate hand piece if necessary; and 
             8. Repeat steps 1-3 until desired result achieved. 
           
         
       
    
     Surrounding structures include, but are not limited to, all bone, cartilage, muscles, tendons, nerves, blood vessels, and epithelium. 
     RF and/or Laser Assisted Wound Regeneration: 
     
         
         
           
             1. Optionally drink 4 oz. diluted Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5 and/or 6; 
             2. Wait 15 minutes; 
             3. Optionally drink 4 oz. diluted Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5 and/or 6; 
             4. Wait 15 minutes; 
             5. Optionally direct RF/laser, RF, LED or laser energy at wound and its surrounding structures; 
             6. Apply or place Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5 and/or 6; 
             7. Optionally direct RF/laser, RF, LED or laser energy at wound and its surrounding structures; 
             8. Keep energy in place for up to 10-20 minutes or until desired effect achieved; 
             9. Rotate energy source if necessary; and 
             10. Repeat steps 5-9 until desired result achieved. 
             11. Alternatively wait a week in between steps 5-9 and gradually cover wound bed. 
             12. Procedure can be started at Step 1 or Step 5. 
           
         
       
    
     Surrounding structures include, but are not limited to, all bone, cartilage, muscles, tendons, nerves, blood vessels, and epithelium. 
     RF and/or Laser Assisted Pore Repair: 
     
         
         
           
             1. Optionally drink 4 oz. diluted Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5; 
             2. Wait 15 minutes; 
             3. Optionally drink 4 oz. diluted Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5; 
             4. Wait 15 minutes; 
             5. Direct RF/laser, RF or laser energy at pores and their surrounding structures; 
             6. Keep energy in place for 10-20 minutes or until desired effect achieved; 
             7. Rotate hand piece; and 
             8. Repeat steps 5-7 until desired result achieved. 
             9. Procedure can be started at Step 1 or Step 5. 
           
         
       
    
     Surrounding structures include, but are not limited to, all bone, cartilage, muscles, tendons, nerves, blood vessels, and epithelium. 
     RF and/or Laser and/or LED Assisted Oral Cavity Wound Repair: 
     
         
         
           
             1. Optionally drink 4 oz. diluted Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5 and/or 6; 
             2. Wait 15 minutes; 
             3. Optionally drink 4 oz. diluted Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5 and/or 6; 
             4. Wait 15 minutes; 
             5. Optionally direct RF/laser, RF or laser energy at oral cavity and its&#39; surrounding structures; 
             6. Optionally apply Substrate 1, and/or 2, and/or 3, and/or 4, and/or 5 and/or 6; 
             7. Optionally direct RF/laser, RF, LED or laser energy wound and its surrounding structures; 8. Keep energy in place for 10-20 minutes or until desired effect achieved; 
             9. Rotate energy source; and 
             8. Repeat steps 5-9 until desired result achieved. 
             9. Procedure can be started at Step 1 or Step 5. 
           
         
       
    
     Surrounding structures include, but are not limited to, all bone, cartilage, muscles, tendons, nerves, blood vessels, and epithelium. 
     Further still, wound treatment may be utilized for additional conditions including, but not limited to, vaginal wound repair, breast wound repair/regeneration/generation, anal wound repair, age spot repair, pore repair, skin and tissue repair and general body wound repair. 
     Referring to  FIG.  33   a - 33   e   , shown is a fourth embodiment of a device  500  for use in conjunction with the substrates and methods of the present invention.  FIG.  33   a    shows a top view of the device  500  of the fourth embodiment.  FIG.  33   b    shows a side view and  FIG.  33   c    shows a close-up of the tip of device  500 . Specifically,  FIG.  33   d    illustrates an exploded view of the device  500  comprised of housing  503 , tips  505  and energy source  507 . Energy source  507  provides RF energy to housing  503  when connected.  FIG.  33   e    shows a side perspective view of the assembled device  500 . Optionally, the device  500  may be used in the absence of substrates. 
     Referring to  FIG.  34   a - 34   f   , shown is a fifth embodiment of a device  600  for use in conjunction with the substrates and methods of the present invention.  FIGS.  34   d  and  34   f    show side perspective views of device  600 .  FIG.  34   a    shows a side view of device  600  wherein device  600  has a hemispheric shape and is further comprised of a flat surface opposite the hemispheric surface. The flat surface is further comprised of a plurality of mini lasers  605  for delivery of diode laser energy for treatment of an acute wound. The mini lasers  605  are self-contained within device  600 . In a preferred embodiment, the laser power used for treatment may be approximately 6-24 mW. 
     Referring to  FIG.  35   a - 35   f   , shown is power device  700  for a fiber optic had piece for use in conjunction with the substrates and methods of the present invention.  FIG.  35   a    shows the components of pocket power device  700 , the pocket power device  700  further comprised of a base  701 , a battery mount  702 , a rechargeable battery  703 , a handle for a fiber optic laser  704 , and a top  705 .  FIG.  35   b - 35   f    show various views of the pocket power device  700 . The handle  704  is a self-contained unit having an attached fiber optic line  706  upon which a fiber optic laser head (not shown) is connected. Further, a diode laser module is housed in pocket power device  700 . 
     Referring to  FIG.  36   a - 36   f   , shown is a portable RF transmitter  800  for use in conjunction with the substrates and methods of the present invention.  FIGS.  36   e  and  36   f    show side perspective views of RF transmitter  800 .  FIG.  36   a    shows a top view;  FIG.  36   b    shows front view;  FIG.  36   c    shows a bottom view; and  FIG.  36   d    shows a left side view. Optionally, the RF transmitter  800  may be used in the absence of substrates. 
     Examples 
     I. Analysis of Tooth #15 at 12 Unique Loci 
     A patient&#39;s pocket depths at tooth 15 were measured at 12 separate loci. The root of the tooth was then scaled and planed to remove calculus build up on the root surface. After scaling and planning, bleeding occurs in the sulcus. The sulcus was allowed to air dry and immediately thereafter the conditioner is applied to the sulcus and left for 30 seconds before being rinsed with saline. The tooth was next scaled and planed again to renew blood flow. With blood pooling in the sulcus, the 45° laser tip was placed into the sulcus. The laser light used has a wavelength in the visible portion of the electromagnetic spectrum, between 400 nm-700 nm wavelength. The laser was emitted continuously with only intermittent stops for tissue temperature control. The laser was allowed to penetrate the entire sulcus by moving the tip vertically and horizontally throughout the sulcus for 30 second. The laser tip was cut to 45° in the opposite angle for the second pass into the sulcus and 90° for the third pass to allow the laser bean to penetrate the existing periodontium to decontaminate and biostimulate the sulcular contents. 
     In the meantime, the first substrate and the second substrate were mixed in a glass dish. Some of the patient&#39;s blood that has been treated with the laser light in the sulcus was also mixed in the glass dish. This mixture is then placed immediately into the sulcus upon mixture. Enough of the mixture was placed into the sulcus to fill the sulcus while ensuring the mixture stayed 3 mm below the top of the gingival margin and remained immersed in blood. The patient&#39;s mouth was kept open for 5 minutes to ensure the newly formed blood clot containing the substrate mixture remained intact. 
     Treatment was repeated on tooth 15 on four subsequent occasions, at which time the pocket depths at each loci were measured prior to treatment. Measurements are shown in  FIG.  11   . The data show an increase in calcium density at the specific loci. 
     II. Analysis of Tooth #12 at 17 Unique Loci 
     A patient&#39;s pocket depths at tooth 28 were measured at 17 separate loci. The treatment disclosed herein was performed on five subsequent occasions, at which time the pocket depths at each loci were measured prior to treatment. Measurements are shown in  FIG.  12   . The data show an increase in calcium density across all loci. 
     III. Analysis of Tooth #2, #3 and #15 at 3 Unique Loci Per Tooth 
     A patient&#39;s pocket depths at tooth 2, tooth 3 and tooth 15 were measured at three separate loci per tooth. The treatment disclosed herein was performed 3 months after the initial treatment, at which time the pocket depths at each loci were measured prior to treatment. Measurements are shown in  FIG.  13   . The data show a progression of bone generation. 
     IV. Analysis of Chin Profile 
     A patient&#39;s chin profile was measured. The treatment disclosed herein was performed once after the initial measurements were taken with measurements repeated following treatment. Measurements are shown in  FIG.  24   . The data show a general increase in chin profile following a single treatment. 
     V. Analysis of Toe Crease 
     A patient&#39;s toe crease length was measured. The treatment disclosed herein was performed after initial measurements were obtained with measurements repeated following treatment. Measurements are shown in  FIG.  25   . The data show a 71% overall decrease in crease size following treatment. 
     VI. Analysis of Gingival Wound Tissue 
     A patient&#39;s gingival wounds were measured from the line to the top of the gingiva. The treatment disclosed herein was performed and measurements were repeated following treatment. Images of gingival wounds are shown before and after treatment in  FIGS.  26   a  and  26   b   . Measurements are shown in  FIG.  26   c   . The data show a 50% or greater decrease in the wound following a single treatment. 
     VII. Analysis of Hand Crease 
     A patient&#39;s hand crease length was measured. The treatment disclosed herein was performed after initial measurements were taken with measurements repeated following treatment. Measurements are shown in  FIG.  27   . The data show an overall decrease in crease length following treatment. 
     VIII. Analysis of New Skin Growth 
     A patient&#39;s skin leg wound was measured. The treatment disclosed herein was performed after initial measurements were taken with measurements repeated following treatment. Measurements are shown in  FIG.  28   . The data show an overall increase in new skin growth following treatment. In a preferred embodiment, chronic wounds on limbs may be treated using a three-sided LED system wherein the treatment unit is placed around the limb on three sides and applies the LED energy to a larger surface area. The LED system uses an energy source less than 500 mW per LED unit of light. 
     IX. Analysis of Anal Scar Reduction 
     A patient&#39;s anal scar tissue was measured. The treatment disclosed herein was performed after initial measurements were taken with measurements repeated following treatment. Measurements are shown in  FIG.  29    and  FIG.  30   . The data show a reduction in both length and width of scar tissue following treatment. 
     X. Analysis of Tongue Strength 
     Tongue strength and swallowing was assessed for three patients. The treatment disclosed herein was performed after initial assessments were made and tongue strength and swallowing were reevaluated following treatment. Measurements are shown in  FIG.  31   . The data show each patient experiencing an increase in tongue strength following treatment. 
     XI. Analysis of Breast Firmness 
     Breast firmness was recorded for two patients. The treatment disclosed herein was performed after initial assessments were made and breast firmness was reevaluated following treatment. Comparative firmness is shown in  FIG.  32   . The data show the patients experiencing an increase in firmness of 75% and 66.7% following treatment, respectively. 
     XII. Analysis of Epithelial Wound Regeneration 
     The epithelial wound regeneration of a patient was assessed. The treatment disclosed herein was performed after initial wound measurement and wound size was remeasured following treatment. Comparative measurements are shown in  FIG.  37   . Epithelial regeneration was found to have increased in area by 170% one day following treatment. 
     XIII. Analysis of Calcaneal Tendon Wound Regeneration 
     The calcaneal tendon wound regeneration of a patient was assessed. The treatment disclosed herein was performed after initial wound measurement and wound size was remeasured following treatment. Comparative measurements are shown in  FIG.  38   . Tendon shelf size increased four times from the initial measurement to the third and final measurement. 
     XIII. Analysis of Ankle Epithelial Wound Regeneration 
     The ankle epithelial wound regeneration of a patient was assessed. The treatment disclosed herein was performed after initial wound measurement and wound size was remeasured following treatment. Comparative measurements for two treatment areas are shown in  FIG.  39   . Epithelial regeneration was found to have increased in area by 264% five months following treatment. 
     XIV. Analysis of Ankle Wound Size Reduction 
     The ankle epithelial wound size reduction of a patient was assessed. The treatment disclosed herein was performed after initial wound measurement and wound size was remeasured following treatment. Comparative measurements for two treatment areas are shown in  FIG.  40   . Epithelial wound size was found to have decreased in area by 72% five months following treatment. 
     XV. Analysis of Oral Cavity Wound Regeneration 
     The oral cavity epithelial wound regeneration of a patient was assessed. The treatment disclosed herein was performed after initial wound measurement and wound size was remeasured following treatment. Comparative measurements are shown in  FIG.  41   . Epithelial regeneration size increased by 1.34 cm 2  from the initial measurement to the fourth and final measurement. 
     XVI. Analysis of Vein Wound Regeneration 
     The vein wound regeneration of a patient was assessed. The treatment disclosed herein was performed after initial wound measurement and wound size was remeasured following treatment. Comparative measurements are shown in  FIG.  42   a   . Epithelial regeneration size increased by 2.9 cm 2  from the initial measurement to final measurement. Treatment was conducted using a flashlight style infrared laser as shown in  FIG.  42   b    wherein the LED beam is a concentrated flat line of light applied to the skin and veins. The flashlight style laser is a self-contained modular laser that allows for manipulation of skin around a wound during treatment. Alternatively, a LED panel may be used. 
     XVII. Analysis of Tissue Regeneration 
     The wound regeneration of a patient was assessed and comparative measurements are shown at  FIG.  43   a   . The wound was to epithelial tissue and the extent of the wound was first measured having an impacted length of 7.70 cm 2 , a width of 2.20 cm 2  and an overall area of 2.42 cm 2 . Six weeks later, the extent of the patient&#39;s wound was substantially reduced to a length of 0.8 cm 2 , a width of 0.7 cm 2  and an overall area of 0.06 cm 2 . Another patient was assessed with a wound in the oral cavity, with results detailed at  FIG.  43   b   . As a measurement of new tissue regenerated, over a four day period, the patient exhibited a significant amount of increased skin area of regenerated tissue, starting with zero regeneration at day zero up to 1.34 cm 2  four days later. 
     One embodiment of the present invention provides a device for treating a wound according to the method described herein, the device emitting a laser a beam of light having a wavelength in the green wavelength range (520-570 nm), red wavelength range (620-750 nm), or yellow wavelength range (570-590 nm) having an alternative wattage of 0.001 W to 5 W, preferably 0.002 W to 4 W, more preferably 0.003 W to 3 W, and most preferably 0.005 W to 2 W. Optionally, the laser light utilizes the IR wavelength range (700 nm-1400 nm) at a laser power of 0.001 W to 5 W to treat wounds. Optionally, a LED light utilizes the IR wavelength range to treat wounds. 
     Another embodiment of the present invention provides a device for treating a wound according to the method described herein, the device emitting a RF beam up to 10 W or, preferably, 9 W comprised of a carrier wave frequency in the range of 0.1 MHz to 20 MHz and a non-sinusoidal waveform in the range of 0.5 to 40 KHz. In a preferred embodiment, the carrier wave frequency is in the range of 0.2 MHz to 10 MHz, preferably 0.3 MHz to 5 MHz. Optionally a 0.001 W to 10 W range RF energy, preferably a 0.001 W to 3 W range, is utilized in the hertz range of 40 Hz to 24 GHz. In a further alternative embodiment, the RF wave is more than one sine wave wherein the more than one demonstrates a harmonics pattern. Optionally, the non-sinusoidal waveform may be in the range of the above parameters as single or multiple waveforms in the presence or absence of a carrier wave. 
     Yet another embodiment of the present invention provides a device for treatment of a wound according to the method described herein, the device emitting a laser beam, a LED beam of light, a RF beam or a combination thereof. 
     Still another embodiment of the present invention provides a device for treatment of wounds in the oral cavity according to the method described herein, the device emitting a fiber optic laser beam. In a preferred embodiment, the fiber optic device may be used in conjunction with the laser, and/or RF device for treating general wounds and wounds of the oral cavity. Optionally, the device emits a LED light. Optionally more than one fiber can be in the handpiece and each fiber can be of a different wavelength and different average power. 
     While there have been shown and described and pointed out the fundamental novel features of the invention as applied to the preferred embodiments, it will be understood that the foregoing is considered as illustrative only of the principles of the invention and not intended to be exhaustive or to limit the invention to the precise forms disclosed. 
     Obvious modifications or variations are possible considering the above teachings. The embodiments discussed were chosen and described to provide the best illustration of the principles of the invention and its practical application to enable one of ordinary skill in the art to utilize the invention in various embodiments and with various modifications as are suited to the particular use contemplated All such modifications and variations are within the scope of the invention as determined by the appended claims when interpreted in accordance with the breadth to which they are entitled.