Patent Publication Number: US-11638421-B2

Title: Oxygen reduction disposable kits, devices and methods of use thereof

Description:
CROSS REFERENCE TO RELATED APPLICATION 
     This application is a continuation of U.S. application Ser. No. 16/375,311, filed Apr. 4, 2019 (now U.S. Pat. No. 11,375,709, issued Jul. 5, 2022) which is a continuation of U.S. application Ser. No. 16/052,738, filed Aug. 2, 2018 (now U.S. Pat. No. 11,350,626, issued Jun. 7, 2022), which is a continuation of U.S. application Ser. No. 15/459,813, filed Mar. 15, 2017 (now U.S. Pat. No. 10,058,091, issued Aug. 28, 2018), which is a continuation of PCT Application No. PCT/US2016/021794 filed Mar. 10, 2016, which claims benefit of U.S. Provisional Application No. 62/131,130 filed Mar. 10, 2015, each of which is hereby incorporated by reference. 
    
    
     FIELD OF THE INVENTION 
     The present disclosure relates to Oxygen Reduction Disposable kits (ORDKit), devices and methods for the improved preservation of whole blood and blood components. More particularly, the disclosure relates to the improved devices and methods for the collection of blood and blood components to provide whole blood and blood components having reduced levels of oxygen. The methods, devices and kits of the present disclosure provide for improved quality of blood and blood components for transfusion and improved patient safety and outcome. 
     BACKGROUND OF THE INVENTION 
     The supplies of liquid blood and blood components are currently limited by storage systems used in conventional blood storage practices. Using current systems, stored blood expires after a period of about 42 days of refrigerated storage at a temperature above freezing (i.e., 4° C.) as packed blood cell preparations. For Example, the World Health Organizaation (WHO) estimates more than 100 million units of blood are collected and stored globally each year. In the US alone, there were 13.6 million units of red blood cells (RBCs) collected in 2013 according to the American Association of Blood Bankers. During refrigerated storage, RBCs become progressively damaged by storage lesions. When transfused within the current 6-week limit, stored RBCs have lower quality as well as potential toxicity, which can be manifested as side effects of transfusion therapy. Among the observed storage lesions are altered biochemical and physical parameters associated with stored red blood cells. Examples of these alterations include in vitro measured parameters such as reduced metabolite levels (adenosine triphosphate (ATP) and 2,3 diphosphoglycerate (2,3-DPG)), increased levels of cell-free iron, hemolysis, increased levels of microparticles, reduced surface area, echinocytosis, phosphatidylserine exposure, and reduced deformability. Expired blood cannot be used and must be discarded because it may harm the ultimate recipient. These reasons and others limit the amount of readily available high quality blood needed for transfusions. 
     When stored conventionally, stored blood undergoes a steady deterioration which is associated with hemolysis, hemoglobin degradation and reduced ATP and 2,3-DPG concentrations. When transfused into a patient, the effects of the steady deterioration during storage manifest, for example, as a reduction in the 24-hour in vivo recovery. Red blood cells stored for an extended period of time under conventional conditions deteriorate and up to 25% may be removed by the recipient&#39;s body shortly after transfusion. Non-viable RBCs cause iron overload in chronically transfused patients. Hemoglobin in RBCs does not release oxygen efficiently at tissues due to loss of 2,3-DPG. RBCs are not able to enter and perfuse capillary beds due to loss of deformability. Storage lesions in transfused blood may lead to major organ failure in the lungs, heart, kidney, liver, and central nervous system, among others. Storage lesions in transfused blood may be associated with increased morbidity. 
     Transfusing RBCs stored under conventional conditions for longer periods may result in higher morbidity and longer hospital stays compared to transfusing “fresher” red cells. Higher morbidity and longer hospital stays result with RBCs that are stored longer than 3 weeks, in comparison to fresher red cells. For example, negative clinical outcomes in cardiac surgery occur when using “older” blood, multiple organ failure in surgical patients is related to the age of transfused red cells, correlations exist between older units and increased mortality in severe sepsis, failure to improve O 2  utilization is attributed to decreased 2,3-DPG, and decreased cardiac index is associated with increased blood viscosity. 
     In addition to immediate removal by the recipient of certain RBCs, consequences of RBC storage lesions include: (i) depletion of ATP (loss of RBC&#39;s ability to dilate the pre-capillary arteriole); (ii) depletion of 2,3-DPG; (iii) accumulation of oxidative damage caused by reactive oxygen species (ROS) formed by the reaction of denatured hemoglobin with O 2 ; and (iv) decreased RBC deformability and increased RBC viscosity, caused in part by oxidative damage to membrane and cytoskeleton. Less deformable RBCs are excluded from capillary channels resulting in low capillary occupancy and reduced tissue perfusion. Massive transfusion of cells with reduced deformability may also contribute to multiple organ failure by blocking the organs&#39; capillary beds. After transfusion, 2,3-DPG is synthesized relatively quickly in vivo to ˜50% of the normal level in as little as 7 hours and to ˜95% of the normal level in 2-3 days. However, since 2,3-DPG-depleted cells do not recover their levels immediately, O 2 -carrying capacity is compromised to the detriment of critically ill patients requiring immediate O 2  delivery and tissue perfusion. There are numerous reports that emphasize the importance of RBCs with high oxygen carrying capacity in such clinical situations. 
     The transfusion of red blood cells (RBCs) is a life-saving therapy aimed at improving oxygenation of the tissues and vital end organs in severely anemic patients. The majority of RBC units used for transfusion are stored at 1-6° C. for up to 42 days in an oxygen-permeable polyvinylchloride blood bag that contains additive/preservative solution. 
     Storage of frozen blood is known in the art, but such frozen blood has limitations. For a number of years, frozen blood has been used by blood banks and the military for certain high-demand and rare types of blood. However, frozen blood is difficult to handle. It must be thawed then cryoprotectant must be gradually washed away which makes it impractical for emergency situations. Once blood is thawed, it must be used within 48 hours. U.S. Pat. No. 6,413,713 to Serebrennikov is directed to a method of storing blood at temperatures below 0° C. 
     U.S. Pat. No. 4,769,318 to Hamasaki et al. and U.S. Pat. No. 4,880,786 to Sasakawa et al. are directed to additive solutions for blood preservation and activation. U.S. Pat. No. 5,624,794 to Bitensky et al., U.S. Pat. No. 6,162,396 to Bitensky et al., and U.S. Pat. No. 5,476,764 to Bitensky are directed to the storage of red blood cells under oxygen-depleted conditions. U.S. Pat. No. 5,789,151 to Bitensky et al. is directed to blood storage additive solutions. For example, Rejuvesol (available from Citra Lab LLC, Braintree, Mass.) is added to blood after cold storage (i.e., 4° C.) just prior to transfusion or prior to freezing (i.e., at −80° C. with glycerol) for extended storage. U.S. Pat. No. 6,447,987 to Hess et al. is directed to additive solutions for the refrigerated storage of human red blood cells. 
     U.S. Pat. No. 4,837,047 to Sato et al. relates to a container for storing blood for a long period of time to keep the quality of the blood in good condition. 
     Traditional manual blood collection is performed by a trained phlebotomist using a blood collection kit that includes, at a minimum, a blood collection bag, a phlebotomy needle, and tubing sufficient to connect the needle to the blood collection bag containing anticoagulant. Typically, a blood collection bag further includes an anticoagulant solution but an anticoagulant solution may alternatively be supplied in a separate bag or container connected to the blood collection bag with suitable tubing. None of the components of current commercial systems provide for, or include, the reduction of oxygen. 
     There is a need to begin the reduction of oxygen from blood prior to storage at the time of collection. In order to accomplish the blood reduction within the existing infrastructure and within the time periods as limited by current regulatory regimes, it is desirable to begin oxygen reduction as early as possible, preferably at collection before the temperature of the collected blood has been significantly reduced. 
     SUMMARY OF THE INVENTION 
     The present disclosure provides for, and includes, an oxygen depletion device for depleting oxygen from blood prior to anaerobic storage comprising an outer receptacle substantially impermeable to oxygen, an inner collapsible blood container comprising one or more chambers that are permeable to oxygen, and an oxygen sorbent situated within said outer receptacle. 
     The present disclosure provides for, and includes, an oxygen depletion device for depleting oxygen from whole blood prior to anaerobic storage comprising an outer receptacle substantially impermeable to oxygen, an inner collapsible blood container comprising one or more chambers that are permeable to oxygen, and an oxygen sorbent situated within said outer receptacle. 
     The present disclosure provides for, and includes, an oxygen depletion device for depleting oxygen from packed red blood cells prior to anaerobic storage comprising an outer receptacle substantially impermeable to oxygen, an inner collapsible blood container comprising one or more chambers that are permeable to oxygen, and an oxygen sorbent situated within said outer receptacle. 
     The present disclosure provides for, and includes, a method to prepare blood for storage comprising providing an oxygen depletion device comprising an outer receptacle substantially impermeable to oxygen, an inner collapsible blood container enclosed within the outer receptacle, and an oxygen sorbent situated between the outer receptacle and inner blood compatible blood container, flowing the blood into the inner collapsible blood container of the oxygen depletion device and producing oxygen-reduced blood having less than 20% oxygen saturation. 
     The present disclosure provides for, and includes, a method to prepare blood for storage comprising providing an oxygen depletion device comprising an outer receptacle substantially impermeable to oxygen, an inner collapsible blood container enclosed within the outer receptacle, and an oxygen sorbent situated between the outer receptacle and inner blood compatible blood container, flowing the blood into the inner collapsible blood container of the oxygen depletion device and producing oxygen-reduced blood having less than 10% oxygen saturation. 
     The present disclosure provides for, and includes, a blood storage device for storing oxygen depleted blood comprising an outer receptacle substantially impermeable to oxygen; an inner collapsible blood container comprising a locating feature adapted to align the collapsible blood container within the geometry of the outer receptacle; at least one inlet comprising tubing connecting to the collapsible blood container and a bond to the outer receptacle, wherein the bond to the outer receptacle is substantially impermeable to oxygen; and an oxygen sorbent situated within the outer receptacle. 
     The present disclosure provides for, and includes an oxygen depletion device  10  for depleting oxygen from blood prior to anaerobic storage comprising an outer receptacle  101  substantially impermeable to oxygen; an oxygen indicator  206 , a spacer material  110 , and about 80 grams of an oxygen sorbent  103  in between an outer receptacle  101  and a 15 μm to 200 μm thick silicone collapsible blood container  102 . 
     The present disclosure provides for, and includes an oxygen depletion device  10  for depleting oxygen from blood prior to anaerobic storage comprising an outer receptacle  101  substantially impermeable to oxygen; an oxygen indicator  206 , a spacer material  110 , and about 80 grams of an oxygen sorbent  103  in between an outer receptacle  101  and a collapsible blood container  102  prepared from PVDF having a 0.2 μm pore size. The present disclosure provides for methods to prepare blood for storage comprising: providing an oxygen depletion device  10  and flowing blood into the inner collapsible blood container  102 , agitating the oxygen depletion device  10  for up to 3 hours, producing oxygen-reduced blood having less than 20% oxygen saturation, and transferring the oxygen-reduced blood to a blood storage device  20 . The method further provides for the production of oxygen-reduced blood having less than 20% oxygen saturation in less than 8 hours after collection from a donor. In a further embodiment, the agitating is nutating. 
     The present disclosure provides for, and includes a method of reducing oxygen from whole blood, or a component thereof, comprising placing the whole blood, or a component thereof in a device  20  comprising a sorbent  207  that has an absorption rate of at least 1.86 cubic centimeters per gram sorbent per hour (cc·g −1 ·hr −1 ), incubating the blood filled device  20  for up to four hours at ambient temperature while agitating at least once per second by translation of at least 3 cm; transferring the blood filled device  20  to storage at 4 to 6° C. In a further aspect, the blood filled device  20  is stored at 4 to 6° C. for up to 42 days. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       Some aspects of the disclosure are herein described, by way of example only, with reference to the accompanying drawings. With specific reference now to the drawings in detail, it is stressed that the particulars shown are by way of example and are for purposes of illustrative discussion of embodiments of the disclosure. In this regard, the description, taken with the drawings, makes apparent to those skilled in the art how aspects of the disclosure may be practiced. 
         FIGS.  1 A-C  illustrate an exemplary embodiment of an oxygen depletion device according to the present disclosure having two compartments arranged side by side. 
         FIGS.  2 A and  2 B  illustrate an exemplary embodiment of an oxygen depletion device according to the present disclosure having three compartments arranged side by side. 
         FIGS.  3 A and  3 B  illustrate an exemplary embodiment of an anaerobic storage bag according to the present disclosure. 
         FIGS.  4 A and  4 B  illustrate an exemplary embodiment of an oxygen reduction disposable storage system having a blood depletion device having two or three compartments, respectively, and an anaerobic storage bag according to the present disclosure. 
         FIG.  5    is a graph of sO 2  reduction in exemplary oxygen depletion devices according to the methods of the present disclosure. 
         FIGS.  6 A and  6 B  illustrates exemplary embodiments of an anaerobic storage bag according to the present disclosure. 
         FIG.  7    illustrates an exemplary embodiment of tie layers  105  joining membranes  113  and  114  in a two-step process according to the present disclosure. 
         FIGS.  8 A and  8 B  illustrate an exemplary embodiment of a spacer  110  comprising inner mesh  117  coextruded with binder mesh  118  and joined to a membrane  113  ( 114 ) according to the present disclosure. 
         FIGS.  9 A and  9 B  illustrate an exemplary embodiment of an anaerobic storage bag having a tie layer  105  joining membranes  113  and  114  ( 9 A) and tie layers  105  applied to membranes  113  and  114  providing a seal  108  wherein the tie layers  105  extend beyond the seal  108  by a distance  109  ( 9 B) according to the present disclosure. 
         FIGS.  10 A to  10 D  illustrate exemplary embodiments of a tie layer  105  having geometric features  121  according to the present disclosure and further comprising a mixing structure  109  as shown in  10 C and  10 D. 
         FIG.  11    illustrates an exemplary embodiment of a collapsible blood container having a spacer  110 , a tie layer  105 , and a geometric feature  121  according to the present disclosure. 
         FIG.  12    is a graph of sO 2  reduction in an exemplary oxygen depletion device according to the methods of the present disclosure. 
         FIG.  13    is a graph of sO 2  reduction in an exemplary inner collapsible blood container  102  with various blood volumes, according to the methods of the present disclosure. 
         FIG.  14    is a graph of sO 2  reduction in an exemplary oxygen depletion device according to the methods of the present disclosure. 
         FIG.  15    is a graph of sO 2  reduction in an exemplary oxygen depletion device having different surface areas according to the methods of the present disclosure. 
         FIG.  16    is a graph showing the effect of spacer  110  on sO 2  reduction in an exemplary oxygen depletion device according to the present disclosure. 
     
    
    
     Corresponding reference characters indicate corresponding parts throughout the several views. The examples set out herein illustrate several embodiments of the invention but should not be construed as limiting the scope of the invention in any manner. 
     In light of current technology, there is a need to improve the quality of blood and blood components such as red blood cells that are to be stored and to extend the storage life of such blood and blood components in advance of transfusion to help minimize morbidity associated with transfusions. In order to conform with regulatory requirements and to ensure reliability, the preparation and processing of the red blood cells must be completed within a limited time period. Further, the process of preparing reduced oxygen blood and blood components must not introduce lesions, including but not limited to, hemolysis of the blood. Finally, there is a need for methods and devices that are compatible with existing anticoagulant and additive solutions to yield improved quality blood and blood components. 
     DETAILED DESCRIPTION 
     To address such needs and others, the present disclosure includes and provides devices and methodology for the preservation of blood and blood components in which the preparation of oxygen reduced blood and blood components is initiated at the donor collection stage. 
     Before explaining at least one aspect of the disclosure in detail, it is to be understood that the disclosure is not necessarily limited in its application to the details set forth in the following description or exemplified by the Examples. The disclosure is capable of other aspects or of being practiced or carried out in various ways. 
     As used herein, the term “bag” refers to collapsible containers prepared from a flexible material and includes pouches, tubes, and gusset bags. As used herein, and included in the present disclosure, the term includes folded bags having one, two, three, or more folds and which are sealed or bonded on one, two, three, or more sides. Bags may be prepared using a variety of techniques known in the art including bonding of sheets of one or more materials. Methods of bonding materials to form bags are known in the art. Also included and provided for in the present disclosure are containers prepared by injection and blow molding. Methods to prepare blow molded and injection molded containers are known in the art. Preferred types of blow molded or injection molded containers are flexible containers that can be reduced in size for efficient packing and shipping while being capable of expanding to accommodate blood or blood components for reduction of oxygen. They also may be designed to conform to the volume of the blood until they are fully expanded. As used throughout the present disclosure, the bags are a form of collapsible container and the two terms are used interchangeably throughout the present disclosure. 
     As used herein, the term “collapsible container” includes bags, containers, enclosures, envelopes, pouches, pockets, receptacles, and other devices that can contain and retain a liquid or fluid. In certain aspects, the collapsible container may be manufactured by conventional means such as injection molding or insert molding. In other aspects, the collapsible container may be prepared from sheets of polymer materials that are bonded together using methods known in the art to prepare containers capable of holding a volume. Such collapsible containers are well known in the art. See, for example, U.S. Pat. No. 3,942,529 issued to Waage; U.S. Pat. No. 4,131,200 issued to Rinfret; and U.S. Pat. No. 5,382,526 issued to Gajewski et al. Suitable methods for bonding polymer materials to prepare collapsible containers according to the present disclosure include heat welding, ultrasonic welding, radio frequency (RF) welding, and solvent welding. In certain aspects, multiple bonding methods may be used to prepare collapsible containers according to the present disclosure. Collapsible containers according to the present disclosure include enclosures having one or more pleats, folds, diaphragms, bubbles, and gussets. Methods for preparing collapsible containers are known in the art. See, for example, U.S. Pat. No. 3,361,041 issued to Grob; U.S. Pat. No. 4,731,978 issued to Martensson; U.S. Pat. No. 4,998,990 issued to Richter et al.; and U.S. Pat. No. 4,262,581 issued to Ferrell. Also included and provided for in the present disclosure are containers having combinations of both flexible and inflexible parts, wherein the flexible parts allow for the expansion of the volume through, for example, pleats, folds or gussets and other similar geometric features in the packaging shape, whereas the inflexible parts may provide rigidity and geometry definition to the container. Methods and designs for preparing collapsible containers having both flexible and inflexible parts are known in the art, such as described by Randall in U.S. Pat. No. 6,164,821 and by LaFleur in U.S. Pat. No. 5,328,268. 
     As used herein the term “about” refers to ±10%. 
     The terms “comprises,” “comprising,” “includes,” “including,” “having,” and their conjugates mean “including but not limited to.” 
     The term “consisting of” means “including and limited to.” 
     The term “consisting essentially of” means that the composition, method or structure may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure. 
     As used herein, the singular forms “a,” “an,” and “the” include plural references unless the context clearly dictates otherwise. For example, the term “a compound” or “at least one compound” may include a plurality of compounds, including mixtures thereof. 
     Throughout this application, various embodiments of this disclosure may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the disclosure. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as “from 1 to 6” should be considered to have specifically disclosed subranges such as “from 1 to 3,” “from 1 to 4,” “from 1 to 5,” “from 2 to 4,” “from 2 to 6,” “from 3 to 6,” etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range. 
     Whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range. The phrases “ranging/ranges between” a first indicate number and a second indicate number and “ranging/ranges from” a first indicate number “to” a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween. 
     As used herein the term “method” refers to manners, means, techniques, and procedures for accomplishing a given task including, but not limited to, those manners, means, techniques, and procedures either known to or readily developed from known manners, means, techniques, and procedures by practitioners of the chemical, pharmacological, biological, biochemical, and medical arts. 
     The present disclosure provides for, and includes, an oxygen depletion device  10  for depleting oxygen from blood comprising an outer receptacle  101  substantially impermeable to oxygen, inner collapsible blood container  102  that is permeable to oxygen, and an oxygen sorbent  103  situated within outer receptacle  101 . 
     The present disclosure also provides for, and includes, oxygen depletion devices  10  configured to be a blood collection and oxygen depletion device  10 . Oxygen depletion devices configured to collect and reduce blood oxygen differ from the oxygen depletion device  10  as described throughout this specification in that a blood collection and oxygen depletion device  10  further includes an anticoagulant to prevent coagulation of the whole blood during the collection process. In certain aspects, the anticoagulant solution of a blood collection and oxygen depletion device  10  is provided in the blood collection and oxygen depletion device  10 . Accordingly, included anticoagulant solutions are also oxygen depleted anticoagulant solutions. In the alternative, anticoagulant solutions may be included separately, either as oxygen depleted solutions or solutions having oxygen. A blood collection and oxygen depletion device  10  is intended to be used with whole blood collected from a donor. As used throughout the present disclosure, the oxygen and depletion device  10  includes and provides for blood collection and oxygen depletion device  10 . The two terms can be, and are, used interchangeably. 
     As used herein, the outer receptacles are prepared from materials that are substantially impermeable to oxygen and optionally impermeable to carbon dioxide. In certain aspects, an outer receptacle  101  is prepared from flexible film materials. In other aspects, an outer receptacle  101  is prepared from a stiff, or inflexible film material. 
     The present disclosure provides for, and includes, an outer receptacle  101  substantially impermeable to oxygen. As used herein, an outer receptacle  101  that is substantially impermeable to oxygen is sufficiently impermeable to oxygen to allow no more than 10 cc of oxygen inside the receptacle over a period of 3 months, and more preferably no more than 5 cc of oxygen over 6 months. As used herein, the term substantially impermeable to oxygen (SIO) refers to materials and compositions that provide a barrier to the passage of oxygen from one side of the barrier to the other, sufficient to prevent significant increases in the partial pressure of oxygen. 
     It is notable that few materials provide complete impermeability and that even the high impermeability of materials can be compromised when joining, welding, folding, and otherwise assembling an outer receptacle  101 . As will be discussed below, oxygen depletion device  10  may further incorporate one or more inlets/outlets  30  comprising a tube  301  and a bond  302  to the outer receptacle  101  (or outer receptacle  201  described below). The outer receptacle  101  must also be designed to accommodate changes in volume of the inner collapsible blood container  102 . Accordingly, special care is taken to incorporate specific design elements and manufacturing methods to ensure the integrity of the impermeable barrier. 
     The present disclosure also provides for, and includes, an outer receptacle  101  that is substantially impermeable to oxygen having a permeability to oxygen of less than about 1.0 cc of oxygen per square meter per day. In certain aspects, a film suitable for use in the preparation of an outer receptacle and other elements of the present disclosure are materials characterized by a Barrer value of less than about 0.140 Barrer. 
     Materials and methods to prepare an outer receptacle  101  are known in the art. See, for example, U.S. Pat. No. 7,041,800 issued to Gawryl et al., U.S. Pat. No. 6,007,529 issued to Gustafsson et al., and U.S. Patent Application Publication No. 2013/0327677 by McDorman, each of which are hereby incorporated by reference in their entireties. Impermeable materials are routinely used in the art and any suitable material can be used. In the case of molded polymers, additives are routinely added to enhance the oxygen (and CO 2 ) barrier properties. See, for example, U.S. Pat. No. 4,837,047 issued to Sato et al. For example, U.S. Pat. No. 7,431,995 issued to Smith et al. describes an oxygen- and carbon dioxide-impermeable receptacle composed of layers of ethylene vinyl alcohol copolymer and modified ethylene vinyl acetate copolymer, impermeable to oxygen and carbon dioxide ingress. In another aspect, the outer receptacle  101  is impermeable to oxygen and carbon dioxide. 
     In certain aspects, films that are substantially impermeable to oxygen may be laminated films. In an aspect, a laminated film that is substantially impermeable to oxygen is a laminated foil film. Film materials can be polymers or foil materials or multilayer constructions that are combinations of foils and polymers. In an aspect, a laminated film may be a polyester membrane laminated with aluminum. An example of suitable aluminum laminated film, also known as a laminated foil, that is substantially impermeable to oxygen is known in the art. For example, U.S. Pat. No. 4,798,728 to Sugisawa discloses aluminum laminated foils of nylon, polyethylene, polyester, polypropylene, and vinylidene chloride. Other laminated films are known in the art. For example, U.S. Pat. No. 7,713,614 to Chow et al. discloses multilayer containers comprising an ethylene-vinyl alcohol copolymer (EVOH) resin that is substantially impermeable to oxygen. In an aspect, an outer receptacle  101  may be a barrier bag constructed by sealing three or four sides by means of heat sealing. The bag is constructed of a multilayer construction that includes materials that provide enhancement to O 2  and CO 2  barrier properties. The bag is constructed of a multilayer construction that includes materials that provide enhancement to O 2  and CO 2  barrier properties. Such materials include the Rollprint Clearfoil® V2 film, having an oxygen transmission rate of 0.01 cc/100 in 2 /24 hrs., Rollprint Clearfoil® X film, having an oxygen transmission rate of 0.004 cc/100 in 2 /24 hrs. and Clearfoil® Z film having an oxygen transmission rate of 0.0008 cc/100 in 2 /24 hrs. (Rollprint Packaging Products, Addison, Ill.). Other manufacturers make similar products with similar oxygen transmission rates, such as Renolit Solmed Wrapflex® films (American Renolit Corp., City of Commerce, Calif.). An example of suitable aluminum laminated film, also known as a laminated foil, that is substantially impermeable to oxygen is obtainable from Protective Packaging Corp. (Carrollton, Tex.). 
     Another approach applicable to the preparation of SIO materials includes multilayer graphitic films made by gentle chemical reduction of graphene oxide laminates with hydroiodic and ascorbic acids. See Su et al., “Impermeable barrier films and protective coatings based on reduced graphene oxide,” Nature Communications 5, Article number: 4843 (2014), hereby incorporated by reference in its entirety. Nanoparticles to enhance oxygen barrier properties are also known in the art, for example, the multilayer barrier stack films provided by Tera-Barrier (Tera-Barrier Films Pte, Ltd, The Aries, Singapore) and described by Rick Lingle in Packaging Digest Magazine on Aug. 12, 2014. 
     In aspects according to the present disclosure, an outer receptacle  101  may be prepared from a gas impermeable plastic. In an embodiment, the gas impermeable plastic may be a laminate. In certain embodiments, the laminate may be a transparent barrier film, for example, a nylon polymer. In embodiment, the laminate may be a polyester film. In an embodiment, the laminate may be Mylar®. In certain embodiments, the laminate may be a metalized film. In an embodiment, the metalized film may be coated with aluminum. In another embodiment, the coating may be aluminum oxide. In another embodiment, the coating may be an ethylene vinyl alcohol copolymer (EVOH) laminated between layers of low density polyethylene (LDPE). 
     An outer receptacle  101  of the present disclosure may be formed of one or more parts prepared from a gas impermeable material including a plastic or other durable lightweight material. In some embodiments, an enclosure may be formed of more than one material. In an embodiment, an outer receptacle  101  may be formed of a material and coated with a gas impermeable material to prepare a gas impermeable enclosure. In an embodiment, a rigid or flexible outer receptacle  101  may be prepared from a plastic that may be injection molded. In embodiments according to the instant disclosure, the plastic may be selected from polystyrene, polyvinyl chloride, or nylon. In an embodiment, outer receptacle  101  materials may be selected from the group consisting of polyester (PES), polyethylene terephthalate (PET), polyethylene (PE), high-density polyethylene (HDPE), polyvinyl chloride (PVC), polyvinylidene chloride (PVDC), low-density polyethylene (LDPE), polypropylene (PP), polystyrene (PS), high impact polystyrene (HIPS), polyamides (PA) (e.g., nylon), acrylonitrile butadiene styrene (ABS), polycarbonate (PC), polycarbonate/acrylonitrile butadiene styrene (PC/ABS), polyurethanes (PU), melamine formaldehyde (MF), plastarch material, phenolics (PF), polyetheretherketone (PEEK), polyetherimide (PEI) (Ultem), polylactic acid (PLA), polymethyl methacrylate (PMMA), polytetrafluoroethylene (PTFE), urea-formaldehyde, and ethylene vinyl alcohol copolymer (EVOH). In certain embodiments, the outer receptacle  101  may be polyethylene. In some embodiments, the polyethylene outer receptacle  101  may comprise one or more polyethylene components that are welded together. In certain aspects, the outer receptacle is comprised of a multilayer film having a polyethylene outer layer, a polyester inner layer, and an aluminum oxide barrier layer dispersed between the inner and outer layers, for example, the Clearfoil® Z film having an oxygen transmission rate of 0.0008 cc/100 in 2 /24 hrs. (Rollprint Packaging Products, Addison, Ill.). 
     The present disclosure provides for, and includes, the preparation of outer receptacles  101  from a film and inner collapsible blood container  102  from a membrane. As used herein, membranes generally refer to materials used to prepare an inner collapsible blood container  102  and films are used to refer to materials used to prepare outer receptacle  101 . While it is understood that certain materials may be referred by the manufacturer as a “membrane” or may be generally known as a “membrane”, for clarity, unless otherwise indicated a film is considered substantially impermeable. A membrane comprises one or more layers of materials in the form of a sheet that allows one or more substances to pass through from one side of the sheet to the other side of the sheet. As used herein, membranes may also be prepared as tubes suitable for connecting together components of oxygen depletion devices  10 , blood collection kits, or connecting together elements of blood collection devices, additive solution bags, leukocyte reduction filters, and anaerobic storage bags. As used throughout, it is understood that a membrane of the present disclosure may be formed as a sheet or a tube depending on the application. Also as previously provided, films to prepare outer receptacles  101  are substantially impermeable to oxygen while an inner collapsible blood container  102  is permeable to oxygen. As used herein, films may also be prepared as tubes suitable for connecting together components of oxygen depletion devices  10 , blood collection kits, or connecting together elements of blood collection devices, additive solution bags, leukocyte reduction filters, and anaerobic storage bags. As used herein the outer receptacles  101  contain all embodiments of  102  as further described herein. 
     As used herein, an inner collapsible blood container  102  is permeable to oxygen. In certain aspects, an inner collapsible blood container  102  is permeable to oxygen and carbon dioxide. In other aspects, an inner collapsible blood container  102  is impermeable to oxygen and permeable to carbon dioxide. 
     The present disclosure provides for and includes the preparation of outer receptacles  101  using heat sealing, blow molding, and injection molding techniques. Suitable materials for preparing outer receptacles  101  using heat sealing, blow molding, and injection molding include PET, standard and multilayer, polypropylene, polyethylene, polycarbonate, ABS, and other polymers known to those skilled in the art. Methods to prepare blow molded and injection molded outer receptacles  101  are known in the art, for example, a multilayer structure comprised of a barrier layer of ethylvinyl alcohol (EVOH) or ethylvinylacetate (EVA) situated between two layers of polypropylene (PP) and offered by Kortec (Kortec, Inc., Rowley, Mass.) and also as described in U.S. Pat. No. 5,906,285 issued to Slat. Additives that strengthen the oxygen and CO 2  barrier properties of the polymers prior to molding or during their formulation or during setup are known in the art. One example is multilayer polymer co-injection resulting in a multilayer PET. Such a barrier resin is typically incorporated at the preform stage as an inner layer with PET on both sides, making PET the liquid contact layer as well as the outside layer. As provided below, suitable blow molded or injection molded outer receptacles  101  are impermeable to oxygen. In certain aspects, suitable heat sealed, blow molded, or injection molded outer receptacles  101  are substantially impermeable to both oxygen and carbon dioxide. 
     The present disclosure provides for, and includes, two types of materials for the preparation of either permeable membranes or substantially impermeable films. In an aspect, permeable membranes according to the present disclosure provide for the passage of substances through the material, specifically but not necessarily exclusively, oxygen. In certain aspects, membranes are selected to permit the passage of oxygen and carbon dioxide while preventing the passage of water, proteins, salts (e.g., plasma components) and cells (e.g., red blood cells, white blood cells, and platelets). The rate of passage through a material depends on one or more properties including particle size, phase of material (liquid vs. gas), hydrophilicity, hydrophobicity, or solubility. The rate of passage, or flux, through a material also depends on the presence or absence of a driving force such as a difference in pressure (or partial pressure), differences in temperature, or differences in concentration between one side of the membrane and the other. The flux through a membrane is known as the membrane permeation flux. The membrane permeation flux of substances through a membrane is inversely proportional to the thickness of the membrane. 
     Membrane permeation flux, for a gas, is defined as the volume flowing through the membrane per unit area per unit time. The SI unit used is m 3 /m 2 ·s. For gases and vapors, the volume is strongly dependent on pressure and temperature. Accordingly, permeation fluxes for gases are often given in terms of standard temperature and pressure (STP) which is defined as 0° C. and 1 atmosphere (1.0013 bar) (e.g., 273° K and 760 torr). As noted above, the rate of passage depends on a driving force or difference between the two sides of the membrane, and this dependence is incorporated in the permeability coefficient, P, or simply the permeability. 
     Permeability (P) is defined as the permeability flux per unit of driving force per unit of membrane thickness. The SI unit for the permeability coefficient P is provided in Table 1. A common unit for gas separation, as in the present disclosure, is the Barrer and is also presented in Table 1. The term cm 3  gas (STP)/cm 2 s refers to the volumetric trans-membrane flux of the diffusing species in terms of standard conditions of 0° C. and 1 atmosphere pressure, the term cm refers to the membrane thickness, and cm-Hg refers to the trans-membrane partial pressure driving force for the diffusing species. Permeability must be experimentally determined. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Permeability Units 
               
            
           
           
               
               
            
               
                   
                 Units of Permeability 
               
               
                   
               
               
                 “Volumetric” permeability 
                 
                   
                     
                       
                         
                           
                             1 
                           
                           ⁢ 
                           
                                 
                           
                           Barrer 
                         
                         = 
                         
                           
                             1 
                             ⁢ 
                             
                               
                                 0 
                                 
                                   
                                     - 
                                     1 
                                   
                                   ⁢ 
                                   0 
                                 
                               
                               · 
                               
                                 cm 
                                 3 
                               
                             
                             ⁢ 
                                 
                             gas 
                             ⁢ 
                               
                             
                               
                                 ( 
                                 STP 
                                 ) 
                               
                               · 
                               
                                 ( 
                                 
                                   cm 
                                   ⁢ 
                                       
                                   membrane 
                                   ⁢ 
                                       
                                   thickness 
                                 
                                 ) 
                               
                             
                           
                           
                             
                               ( 
                               
                                 
                                   cm 
                                   2 
                                 
                                 ⁢ 
                                     
                                 membrane 
                                 ⁢ 
                                     
                                 area 
                               
                               ) 
                             
                             · 
                             s 
                             · 
                             
                               ( 
                               
                                 cm 
                                 ⁢ 
                                 Hg 
                                 ⁢ 
                                     
                                 pressure 
                               
                               ) 
                             
                           
                         
                       
                     
                   
                 
               
               
                   
               
               
                 “Molar” permeability 
                 
                   
                     
                       
                         
                           
                             mol 
                             
                               m 
                               · 
                               Pa 
                               · 
                               s 
                             
                           
                           ⁢ 
                           
                             ( 
                             
                               SI 
                               ⁢ 
                                   
                               units 
                             
                             ) 
                           
                         
                         = 
                         
                           
                             
                               ( 
                               
                                 
                                   mol 
                                   i 
                                 
                                 ⁢ 
                                     
                                 permeating 
                               
                               ) 
                             
                             · 
                             
                               ( 
                               
                                 m 
                                 ⁢ 
                                     
                                 membrane 
                                 ⁢ 
                                     
                                 thickness 
                               
                               ) 
                             
                           
                           
                             
                               ( 
                               
                                 
                                   m 
                                   2 
                                 
                                 ⁢ 
                                     
                                 membrane 
                                 ⁢ 
                                     
                                 area 
                               
                               ) 
                             
                             · 
                             s 
                             · 
                             
                               ( 
                               
                                 Pa 
                                 ⁢ 
                                     
                                 pressure 
                               
                               ) 
                             
                           
                         
                       
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Membranes suitable for the methods and devices according to the present disclosure include dense membranes, porous membranes, asymmetric membranes, and composite membranes. In certain aspects, suitable membranes may be multilayered membranes. In other aspects, suitable membranes are prepared from inorganic materials. Dense membranes are membranes prepared from solid materials that do not have pores or voids. Materials permeate dense membranes by processes of solution and diffusion. Examples of dense membranes include silicone membranes (polydimethyl siloxane, or PDMS). Also included and provided for in the present disclosure are porous membranes that have pores of a particular range of sizes that separate on the basis of size exclusion. Examples of porous membranes suitable for use according to the present disclosure include PVDF and polysulfone membranes. 
     Included and provided for by the present disclosure are composite membranes that are made of more than one material, often as laminates, wherein a dense material is applied to a porous support layer. Examples of composite membranes suitable for use according to the present disclosure are EMD Millipore&#39;s GVHP hydrophobic PVDF having 1.0 μm or 0.22 μm pore sizes. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Permeability of Fluoropolymers (100 μm thick; 23° C.) 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 Silicone 
                 PTFE 
                 PFA 
                 FEP 
                 ETFE 
                 CTFE 
                 ECTFE 
                 PVDF 
                 PVF 
                 THV 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 Water vapor 
                 36000 
                 5 
                 8 
                 1 
                 2 
                 1 
                 2 
                 2 
                 7 
                 1.73 
               
               
                 (g/m 2  · d · bar) 
               
               
                 Oxygen 
                 500 
                 1500 
                 n/a 
                 2900 
                 350 
                 60 
                 100 
                 20 
                 12 
                 696 
               
               
                 (cm 3 /m 2  · d · bar) 
               
               
                 Nitrogen 
                 280 
                 500 
                 n/a 
                 1200 
                 120 
                 10 
                 40 
                 30 
                 1 
                 217 
               
               
                 (cm 3 /m 2  · d · bar) 
               
               
                 CO 2   
                 2700 
                 15000 
                 7000 
                 4700 
                 1300 
                 150 
                 400 
                 100 
                 60 
                 2060 
               
               
                 (cm 3 /m 2  · d · bar) 
               
               
                   
               
               
                 From Kunststoffe “Fluorocarbon films - Present situation and Future Outlook” available at kynar.com 
               
            
           
         
       
     
     The present disclosure provides for, and includes, inner collapsible blood containers  102  prepared from membranes  113  that are characterized primarily by their permeability to oxygen. Unless indicated otherwise, a “substantially impermeable membrane” refers to membranes that are substantially impermeable to oxygen. However, in certain devices and methods, the membranes may be further characterized by the permeability or impermeability to carbon dioxide. For certain applications, the membrane material is substantially impermeable to oxygen and provides a barrier to the introduction of oxygen to the blood, blood component, or a blood collection kit comprised of multiple components. Such substantially impermeable membranes are generally used to prepare outer receptacles of the present disclosure. Suitable substantially impermeable membranes may also be used to prepare tubing for connective components of the devices and kits. Substantially impermeable membranes may comprise a monolayer or be laminated sheets or tubes having two or more layers. 
     The present disclosure also provides for, and includes, membranes  113  that are substantially permeable to oxygen. Membranes  113  that are substantially permeable to oxygen are used in the present disclosure for the preparation of inner collapsible blood containers  102 . In certain aspects, the membranes  113  that are permeable to oxygen are also biocompatible membranes, approved and suitable for extended contact with blood that is to be transfused into a patient. Like substantially impermeable membranes, substantially permeable membranes  113  may comprise a monolayer or may comprise a laminated structure having two or more layers. 
     In an aspect, oxygen permeable membranes  113  having a permeability to oxygen of greater than about 2.5×10 −9  cm 3  O 2  (STP)/((cm 2  s)*(cm Hg cm −1 )) is used for the preparation of a collapsible blood container  102 . In another aspect, oxygen permeable membranes  113  having a permeability to oxygen greater than about 5.0×10 −9  cm 3  O 2  (STP)/((cm 2  s)*(cm Hg cm −1 )) is used for the preparation of a collapsible blood container  102 . In yet another aspect, oxygen permeable membranes  113  have a permeability to oxygen of greater than about 1.0×10 −8  cm 3  O 2  (STP)/((cm 2  s)*(cm Hg cm −1 )). In certain aspects, oxygen permeable membranes  113  suitable for use in the preparation of a collapsible blood container  102  are characterized by a Barrer value of greater than about 25. In other aspects, oxygen permeable membranes  113  suitable for use in the preparation of a collapsible blood container  102  are characterized by a Barrer value of greater than about 50. In certain other aspects, oxygen permeable membranes  113  suitable for use in the preparation of a collapsible blood container  102  are characterized by a Barrer value of greater than about 100. 
     In an aspect, a membrane  113  that is substantially permeable to oxygen can be dense membranes prepared from non-porous materials. Examples of suitable materials that are capable of high oxygen permeability rates include silicones, polyolefins, epoxies, and polyesters. In another aspect, membranes that are substantially permeable to oxygen can be porous membranes prepared from organic polymers. A membrane  113  that is substantially permeable to oxygen may be prepared from a material selected from the group consisting of PVDF rendered hydrophobic, nylon, cellulose esters, polysulfone, polyethersulfone, polypropylene rendered hydrophobic, and polyacrylonitrile. 
     The present disclosure provides for, and includes, preparing membranes  113  that are substantially permeable to oxygen, not only by selecting the material, but also by selecting and controlling the thickness. As provided above, permeability is proportional to the thickness of the membrane. Accordingly, improved permeability may be achieved by decreasing the thickness of the membrane. In certain aspects, the minimum thickness is determined by its strength and resistance to puncture and tearing. 
     The present disclosure also provides for, and includes, membranes  113  that are substantially permeable to oxygen that are prepared using blow molding and injection molding techniques. Suitable materials for preparing inner collapsible blood containers  102  using blow molding and injection molding include silicone materials such as Bluestar 4350, 50 durometer, Silbione grade liquid silicone rubber and Shin-Etsu KEG-2000-40A/B Liquid Silicone. The silicone durometer choice is carefully chosen for collapsibility and permeability, followed by a well-controlled wall thickness. Thinner materials will have a higher permeability. Methods to prepare blow molded and injection molded collapsible blood containers  102  are known in the art, for example, U.S. Pat. No. 4,398,642 issued to Okudaira et al.; U.S. Pat. No. 7,666,486 issued to Sato et al.; U.S. Pat. No. 8,864,735 issued to Sano et al.; and U.S. Patent Application Publication No. 2012/0146266 by Oda et al. In an aspect, a blow molded collapsible blood container  102  can be prepared using LDPE used in the manufacture of collapsible water containers. As provided below, suitable blow molded or injection molded collapsible blood containers  102  have a permeability to oxygen of at least about 25 Barrer. 
     In an aspect according to the present disclosure, the collapsible blood container  102  can be manufactured from microporous membrane  113  by various sealing methods such as heat sealing, thermal staking, and adhesive bonding. In one aspect according to the present disclosure, a pair of PVDF microporous membranes are bonded together around the periphery with a section of PVC inlet tubing in place in the seam using an adhesive such as Loctite 4011 in conjunction with an adhesive primer such as Loctite 770. In another aspect according to the present disclosure, a collapsible blood container can be manufactured from a pair of microporous membranes by heat sealing the 4 edges of the pair of membranes together with a section of multilayer tubing sealed into the seam to provide for fluid connectivity. 
     The present disclosure provides for, and includes, a collapsible blood container  102  that is prepared from more than one type of membrane  113 . In an aspect, a collapsible blood container  102  comprises a first membrane  113  and a second membrane  114  suitably bonded to prepare a container. As used herein, a membrane  114  generally refers to a membrane that is identical to membrane  113 . That is, a collapsible blood container  102  is generally made of two joined membranes  113 . The present disclosure provides for, and includes, a collapsible blood container  102  that is prepared from a membrane  113  and a membrane  114  comprising a different material. As shown in  FIG.  1 C , a collapsible blood container  102  is shown to be prepared with a membrane  113  and a membrane  114 . Unless indicated otherwise, it is understood that a membrane  113  and a membrane  114  may be exchanged. In another aspect, a collapsible blood container  102  comprises a membrane  113  combined with a second membrane  114  that has a permeability of less than about 30% of the permeability of first membrane  113 . In certain aspects, a second membrane  114  comprises a membrane that is relatively impermeable or insufficiently permeable to provide sufficient deoxygenation on its own, but can be combined with a suitable membrane  113 . In certain aspects, the second membrane  114  is relatively impermeable. In further aspects, the second membrane  114  comprises a molded membrane that incorporates ridges, baffles, or other structures to facilitate mixing. In an aspect, the second membrane  114  may comprise a rigid structure joined to an oxygen permeable membrane  113 . In aspects according to the present disclosure, the second membrane  114  is heat sealed to membrane  113 . 
     In certain aspects, the inner collapsible blood container  102  contains flow baffles located internal or external to the blood contact area that provide an increase in the turbulence inside the collapsible blood container  102  when agitated. In an aspect, baffles are located 1 to 2 inches from each other and comprise 10 to 45% of the inner collapsible blood container  102  area. 
     The present disclosure provides for, and includes, a collapsible blood container  102  that is substantially permeable to oxygen and is a microporous membrane prepared from polyvinylidene fluoride, or polyvinylidene difluoride (PVDF). In certain aspects, the PVDF membrane is a hydrophobic microporous membrane that is substantially permeable to oxygen. 
     In aspects according to the present disclosure, the microporous PVDF membrane comprises pores having a range of between 0.01 μm and 2.0 μm. In other aspects, the microporous PVDF membrane  113  comprises pores having a range of between 0.01 μm and 1.0 μm. In some aspects, a microporous PVDF membrane  113  has a pore size of between 0.03 μm and 1.0 μm in diameter. In other aspects, a microporous PVDF membrane  113  has a pore size of between 0.03 μm and 0.45 μm in diameter. 
     In aspects according to the present disclosure, the void fraction of a PVDF membrane  113  used to prepare a collapsible blood container  102  is between 20 and 80%. In another aspect, the void fraction of a PVDF membrane  113  used to prepare a collapsible blood container  102  is between 35 and 50%. 
     In certain aspects, the permeability of PVDF membranes having micropores greater than about 1.0 μm may allow fluid to permeate through the membrane, compromising both the fluid containment as well as the oxygen and carbon dioxide permeability. To overcome this permeability at high pore sizes, so called “super-hydrophobic” membranes can be employed wherein the contact angle is greater than 150°. As used herein and known in the art, the contact angle quantifies the wettability of a solid surface and is theoretically described by Young&#39;s equation. In certain aspects according the present disclosure, the use of non-hydrophobic PVDF materials is not recommended as the surface tension of the material is lower and allows for fluid to seep through the pores even at the ranges stated above. 
     In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a PVDF permeable membrane  113  having a pore size of between 0.1 and 0.8 μm in diameter. In other aspects, micropores of porous PVDF membranes may be from 0.22 to 0.8 μm in diameter. In an aspect, the micropores of porous PVDF membranes are from 0.2 to 1.0 μm. In another aspect, the micropores of porous PVDF membranes may be greater than 0.1 and less than 1.0 μm. In a further aspect, the micropore of the porous PVDF membrane ranges from about 0.05 to about 1.0 μm. In some aspects, the micropores of porous PVDF membranes may be greater than 0.3 or 0.4 μm. In other aspects, the micropores of porous PVDF membranes may be greater than 0.5 or 0.6 μm. 
     In aspects according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PVDF membrane  113  having a micropore size of less than 1.0 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PVDF membrane  113  having a micropore size of less than 0.8 μm. In certain aspects according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PVDF membrane  113  having a micropore size of less than 0.65 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PVDF membrane  113  having a micropore size of less than 0.45 μm. 
     In an aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PVDF membrane  113  having a micropore size of 0.1 μm. In another aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PVDF membrane  113  having a micropore size of 0.22 μm. In another aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PVDF membrane  113  having a micropore size of 0.20 μm. In a further aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PVDF membrane  113  having a micropore size of 0.45 μm. In yet a further aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PVDF membrane  113  having a micropore size of 0.65 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PVDF membrane  113  having a micropore size of 0.8 μm. 
     In aspects according to the present disclosure, the PVDF membrane may be less than 250 μm thick. In certain aspects, the membrane is greater than 10 μm thick. In some aspects, the PVDF membrane may be between 10 and 250 μm thick. In other aspects, the PVDF membrane may be between 10 and 125 μm thick or between 25 and 150 μm thick. In an aspect, the PVDF membrane may be between 50 and 125 μm thick, 75 and 125 μm thick, 50 and 150 μm thick, 75 and 150 μm thick, 100 and 125 μm thick, 150 and 250 μm thick, or between 25 and 150 μm thick. In an aspect, the membrane  113  of inner collapsible blood container  102  is about 20 μm thick. In another aspect, the membrane  113  of inner collapsible blood container  102  is about 30 μm thick. In yet another aspect, the membrane  113  of inner collapsible blood container  102  is about 50 μm thick. In a further aspect, the membrane  113  of inner collapsible blood container  102  is about 76 μm thick. In an aspect, the membrane  113  of inner collapsible blood container  102  is about 120 μm thick. 
     In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a PVDF permeable membrane  113  that is between 100 and 125 μm thick. In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a PVDF permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter and that is between 100 and 125 μm thick. In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a PVDF permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter and that is between 50 and 150 μm thick. 
     Examples of suitable PVDF membranes for the preparation of inner collapsible blood containers that are permeable to oxygen according to the present disclosure include VVSP 115 μm thick/0.1 μm pore; GVSP 115 μm thick/0.22 μm pore; HVSP 115 μm thick/0.45 μm pore; DVSP 115 μm thick/0.65 μm pore; BVSP 115 μm thick/1.0 μm pore; VVHP 107 μm thick/0.1 μm pore; GVHP 125 μm thick/0.22 μm pore; HVHP 115 μm thick/0.45 μm pore; or DVHP 115 μm thick/0.65 μm pore. 
     Suitable PVDF membranes include commercially available membranes. Non-limiting examples of PVDF membranes are available from Millipore Corporation, Bedford, Mass. In an aspect, the PVDF membrane may be obtained from Millipore Corporation, Bedford, Mass. An example of such a PVDF membrane is the VVSP, GVSP, HVSP, DVSP, BVSP, VVHP, GVHP, HVHP, or DVHP. 
     The present disclosure provides for, and includes, a collapsible blood container  102  that is substantially permeable to oxygen and is a microporous membrane prepared from polysulfone. In certain aspects, the polysulfone membrane is a hydrophobic microporous membrane that is substantially permeable to oxygen. 
     In aspects according to the present disclosure, the microporous polysulfone membrane comprises pores having a range of between 0.01 μm and 2.0 μm. In other aspects, the microporous polysulfone membrane  113  comprises pores having a range of between 0.01 μm and 1.0 μm. In some aspects, a microporous polysulfone membrane  113  has a pore size of between 0.03 μm and 1.0 μm in diameter. In other aspects, a microporous polysulfone membrane  113  has a pore size of between 0.03 μm and 0.45 μm in diameter. 
     In aspects according to the present disclosure, the void fraction of a polysulfone membrane  113  used to prepare a collapsible blood container  102  is between 20 and 80%. In another aspect, the void fraction of a polysulfone membrane  113  used to prepare a collapsible blood container  102  is between 35 and 50%. 
     In certain aspects, the permeability polysulfone membranes having micropores greater than about 0.2 μm may allow fluid to permeate through the membrane, compromising both the fluid containment and the oxygen and carbon dioxide permeability. To overcome this permeability at high pore sizes, so called “super-hydrophobic” membranes can be employed wherein the contact angle is greater than 150°. As used herein and known in the art, the contact angle quantifies the wettability of a solid surface and is theoretically described by Young&#39;s equation. In certain aspects according the present disclosure, the use of non-hydrophobic polysulfone materials is not recommended as the surface tension of the material is lower and allows for fluid to seep through the pores even at the ranges stated above. 
     In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a polysulfone permeable membrane  113  having a pore size of between 0.3 μm and 0.8 μm in diameter. In other aspects, micropores of porous polysulfone membranes may be from 0.22 μm to 0.8 μm in diameter. In an aspect, the micropores of porous polysulfone membranes are from 0.2 μm to 1.0 μm. In another aspect, the micropores of porous polysulfone membranes may be greater than 0.1 μm and less than 1.0 μm. In a further aspect, the micropore of the porous polysulfone membrane ranges from about 0.05 μm to about 1.0 μm. In some aspects, the micropores of porous polysulfone membranes may be greater than 0.3 μm or 0.4 μm. In other aspects, the micropores of porous polysulfone membranes may be greater than 0.5 μm or 0.6 μm. 
     In aspects according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of less than 1.0 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of less than 0.8 μm. In certain aspects according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of less than 0.65 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of less than 0.45 μm. 
     In an aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of 0.1 μm. In another aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of 0.22 μm. In another aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of 0.20 μm. In a further aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of 0.45 μm. In yet a further aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of 0.65 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of 0.8 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of 0.03 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of 0.05 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polysulfone membrane  113  having a micropore size of 1.2 μm. 
     In aspects according to the present disclosure, the polysulfone membrane may be less than 250 μm thick. In certain aspects, the membrane is greater than 10 μm thick. In some aspects, the polysulfone membrane may be between 10 and 250 μm thick. In other aspects, the polysulfone membrane may be between 10 and 125 μm thick or 25 and 150 μm thick. In an aspect, the polysulfone membrane may be between 50 and 125 μm thick, 75 and 125 μm thick, 50 and 150 μm thick, 75 and 150 μm thick, 100 and 125 μm thick, 150 and 250 μm thick, or between 25 and 150 μm thick. In an aspect, the membrane  113  of inner collapsible blood container  102  is about 20 μm thick. In another aspect, the membrane  113  of inner collapsible blood container  102  is about 30 μm thick. In yet another aspect, the membrane  113  of inner collapsible blood container  102  is about 50 μm thick. In a further aspect, the membrane  113  of inner collapsible blood container  102  is about 76 μm thick. In an aspect, the membrane  113  of inner collapsible blood container  102  is about 120 μm thick. 
     In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a polysulfone permeable membrane  113  that is between 100 and 125 μm thick. In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a polysulfone permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter and that is between 100 and 125 μm thick. In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a polysulfone permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter and that is between 50 and 150 μm thick. 
     Examples of suitable polysulfone membranes for the preparation of inner collapsible blood containers that are permeable to oxygen according to the present disclosure include SS003AH 10-250 μm thick/0.03 μm pore; SS005AH 10-250 μm thick/0.05 μm pore; SS010AH 10-250 μm thick/0.1 μm pore; SS020AH 10-250 μm thick/0.2 μm pore; SS045AH 10-250 μm thick/0.45 μm pore; SS065AH 10-250 μm thick/0.65 μm pore; SS080AH 10-250 μm thick/0.8 μm pore; or SS120AH 10-250 μm thick/1.2 μm pore. 
     Suitable polysulfone membranes include commercially available membranes. Non-limiting examples of polysulfone membranes are available from Pacific Membranes. In an aspect, the polysulfone membrane may be SS120AH, SS080AH, SS065AH, SS045AH, SS020AH, SS010AH, SS005AH, or SS003AH. 
     The present disclosure provides for, and includes, a collapsible blood container  102  that is substantially permeable to oxygen and is a microporous membrane prepared from polyolefin. In certain aspects, the polyolefin membrane is a hydrophobic microporous membrane that is substantially permeable to oxygen. 
     In aspects according to the present disclosure, the microporous polyolefin membrane comprises pores having a range of between 0.01 μm and 2.0 μm. In other aspects, the microporous polyolefin membrane  113  comprises pores having a range of between 0.01 μm and 1.0 μm. In some aspects, a microporous polyolefin membrane  113  has a pore size of between 0.03 μm and 1.0 μm in diameter. In other aspects, a microporous polyolefin membrane  113  has a pore size of between 0.03 μm and 0.45 μm in diameter. 
     In aspects according to the present disclosure, the void fraction of a polyolefin membrane  113  used to prepare a collapsible blood container  102  is between 20 and 80%. In another aspect, the void fraction of a polyolefin membrane  113  used to prepare a collapsible blood container  102  is between 35 and 50%. 
     In certain aspects, the permeability polyolefin membranes having micropores greater than about 1.0 μm may allow fluid to permeate through the membrane, compromising both the fluid containment and the oxygen and carbon dioxide permeability. To overcome this permeability at high pore sizes, so called “super-hydrophobic” membranes can be employed wherein the contact angle is greater than 150°. As used herein and known in the art, the contact angle quantifies the wettability of a solid surface and is theoretically described by Young&#39;s equation. In certain aspects according the present disclosure, the use of non-hydrophobic polyolefin materials is not recommended as the surface tension of the material is lower and allows for fluid to seep through the pores even at the ranges stated above. 
     In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a polyolefin permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter. In other aspects, micropores of porous polyolefin membranes may be from 0.22 μm to 0.8 μm in diameter. In an aspect, the micropores of porous polyolefin membranes are from 0.2 μm to 1.0 μm. In another aspect, the micropores of porous polyolefin membranes may be greater than 0.1 and less than 1.0 μm. In a further aspect, the micropore of the porous polyolefin membrane ranges from about 0.05 μm to about 1.0 μm. In some aspects, the micropores of porous polyolefin membranes may be greater than 0.3 or 0.4 μm. In other aspects, the micropores of porous polyolefin membranes may be greater than 0.5 or 0.6 μm. 
     In aspects according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polyolefin membrane  113  having a micropore size of less than 1.0 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polyolefin membrane  113  having a micropore size of less than 0.8 μm. In certain aspects according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polyolefin membrane  113  having a micropore size of less than 0.65 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polyolefin membrane  113  having a micropore size of less than 0.45 μm. 
     In an aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polyolefin membrane  113  having a micropore size of 0.1 μm. In another aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polyolefin membrane  113  having a micropore size of 0.22 μm. In another aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polyolefin membrane  113  having a micropore size of 0.20 μm. In a further aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polyolefin membrane  113  having a micropore size of 0.45 μm. In yet a further aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polyolefin membrane  113  having a micropore size of 0.65 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a polyolefin membrane  113  having a micropore size of 0.8 μm. 
     In aspects according to the present disclosure, the polyolefin membrane may be less than 250 μm thick. In certain aspects, the membrane is greater than 10 μm thick. In some aspects, the polyolefin membrane may be between 10 and 250 μm thick. In other aspects, the polyolefin membrane may be between 10 and 125 μm thick or between 25 and 150 μm thick. In an aspect, the polyolefin membrane may be between 50 and 125 μm thick, 75 and 125 μm thick, 50 and 150 μm thick, 75 and 150 μm thick, 100 and 125 μm thick, 150 and 250 μm thick, or between 25 and 150 μm thick. In an aspect, the membrane  113  of inner collapsible blood container  102  is about 20 μm thick. In another aspect, the membrane  113  of inner collapsible blood container  102  is about 30 μm thick. In yet another aspect, the membrane  113  of inner collapsible blood container  102  is about 50 μm thick. In a further aspect, the membrane  113  of inner collapsible blood container  102  is about 76 μm thick. In an aspect, the membrane  113  of inner collapsible blood container  102  is about 120 μm thick. 
     In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a polyolefin permeable membrane  113  that is between 100 and 125 μm thick. In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a polyolefin permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter and that is between 100 μm and 125 μm thick. In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a polyolefin permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter and that is between 50 μm and 150 μm thick. 
     Examples of suitable polyolefin membranes for the preparation of inner collapsible blood containers that are permeable to oxygen according to the present disclosure include those described in U.S. Pat. No. 4,440,815 issued to Zomorodi et al. 
     The present disclosure provides for, and includes, a collapsible blood container  102  that is substantially permeable to oxygen and is a microporous membrane prepared from polytetrafluoroethylene (PTFE). In certain aspects, the PTFE membrane is a hydrophobic microporous membrane that is substantially permeable to oxygen. 
     In aspects according to the present disclosure, the microporous PTFE membrane comprises pores having a range of between 0.01 μm and 2.0 μm. In other aspects, the microporous PTFE membrane  113  comprises pores having a range of between 0.01 μm and 1.0 μm. In some aspects, a microporous PTFE membrane  113  has a pore size of between 0.03 μm and 1.0 μm in diameter. In other aspects, a microporous PTFE membrane  113  has a pore size of between 0.03 μm and 0.45 μm in diameter. 
     In aspects according to the present disclosure, the void fraction of a PTFE membrane  113  used to prepare a collapsible blood container  102  is between 20 and 80%. In another aspect, the void fraction of a PTFE membrane  113  used to prepare a collapsible blood container  102  is between 35 and 50%. 
     In certain aspects, the permeability PTFE membranes having micropores greater than about 1.0 μm may allow fluid to permeate through the membrane, compromising both the fluid containment and the oxygen and carbon dioxide permeability. To overcome this permeability at high pore sizes, so called “super-hydrophobic” membranes can be employed wherein the contact angle is greater than 150°. As used herein and known in the art, the contact angle quantifies the wettability of a solid surface and is theoretically described by Young&#39;s equation. In certain aspects according the present disclosure, the use of non-hydrophobic PTFE materials is not recommended as the surface tension of the material is lower and allows for fluid to seep through the pores even at the ranges stated above. 
     In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a PTFE permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter. In other aspects, micropores of porous PTFE membranes may be from 0.22 μm to 0.8 μm in diameter. In an aspect, the micropores of porous PTFE membranes are from 0.2 μm to 1.0 μm. In another aspect, the micropores of porous PTFE membranes may be greater than 0.1 and less than 1.0 μm. In a further aspect, the micropore of the porous PTFE membrane ranges from about 0.05 μm to about 1.0 μm. In some aspects that micropores of porous PTFE membranes may be greater than 0.3 or 0.4 μm. In other aspects, the micropores of porous PTFE membranes may be greater than 0.5 or 0.6 μm. 
     In aspects according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PTFE membrane  113  having a micropore size of less than 1.0 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PTFE membrane  113  having a micropore size of less than 0.8 μm. In certain aspects according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PTFE membrane  113  having a micropore size of less than 0.65 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PTFE membrane  113  having a micropore size of less than 0.45 μm. 
     In an aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PTFE membrane  113  having a micropore size of 0.1 μm. In another aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PTFE membrane  113  having a micropore size of 0.22 μm. In another aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PTFE membrane  113  having a micropore size of 0.20 μm. In a further aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PTFE membrane  113  having a micropore size of 0.45 μm. In yet a further aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PTFE membrane  113  having a micropore size of 0.65 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a PTFE membrane  113  having a micropore size of 0.8 μm. 
     In aspects according to the present disclosure, the PTFE membrane  113  may be less than 250 μm thick. In certain aspects, the membrane is greater than 10 μm thick. In some aspects the PTFE membrane  113  may be between 10 and 250 μm thick. In other aspects, the PTFE membrane  113  may be between 10 and 125 or 25 and 150 μm thick. In an aspect, the PTFE membrane  113  may be between 50 and 125 μm thick, 75 and 125 μm thick, 50 and 150 μm thick, 75 and 150 μm thick, 100 and 125 μm thick, 150 and 250 μm thick or between 25 and 150 μm thick. In another aspect, the membrane  113  of inner collapsible blood container  102  is about 30 μm. In yet another aspect, the membrane  113  of inner collapsible blood container  102  is about 50 μm. In a further aspect, the membrane  113  of inner collapsible blood container  102  is about 76 μm. In an aspect, the membrane  113  of inner collapsible blood container  102  is about 120 μm thick, 100 and 125 μm thick, 150 and 250 μm thick or between 25 and 150 μm thick. 
     In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a PTFE permeable membrane  113  that is between 100 and 125 μm thick. In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a PTFE permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter and that is between 100 and 125 μm thick. In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a PTFE permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter and that is between 50 and 150 μm thick. 
     Examples of suitable PTFE membranes for the preparation of inner collapsible blood containers that are permeable to oxygen according to the present disclosure include the Poreflon® FP, WP, and HP series PTFE membranes from Sumitomo Electric Interconnect Products, San Marcos, Calif., and Tetratex® 2 from Donaldson Membranes, Ivyland, Pa. 
     Suitable PTFE membranes include commercially available membranes. Non-limiting examples of PTFE membranes are available from Sumitomo Electric Interconnect Products, San Marcos, Calif., and Donaldson Membranes, Ivyland, Pa. In an aspect, the PTFE membrane may be FP-010 from Sumitomo Electric Interconnect Products, San Marcos, Calif. 
     In certain aspects, suitable membranes that are substantially permeable to oxygen may be multilayered membranes. In certain aspects, the multilayered membranes are hydrophobic microporous membranes that are substantially permeable to oxygen. Suitable multilayered membranes include multilayered membranes having two or more materials selected from the group consisting of PVDF rendered hydrophobic, nylon, cellulose esters, polysulfone, polyethersulfone, polypropylene rendered hydrophobic, and polyacrylonitrile. 
     The present disclosure provides for, and includes, a collapsible blood container  102  that is substantially permeable to oxygen and is a microporous membrane prepared from an extruded, woven, non-woven single layer or multilayered membrane. In certain aspects, the multilayered membrane is a hydrophobic microporous membrane that is substantially permeable to oxygen. 
     In aspects according to the present disclosure, the microporous multilayered membrane comprises pores having a range of between 0.01 micrometer (μm) and 2.0 μm. In other aspects, the microporous multilayered membrane  113  comprises pores having a range of between 0.01 μm and 1.0 μm. In some aspects, a microporous multilayered membrane  113  has a pore size of between 0.03 μm and 1.0 μm in diameter. In other aspects, a microporous multilayered membrane  113  has a pore size of between 0.03 μm and 0.45 μm in diameter. 
     In aspects according to the present disclosure, the void fraction of a multilayered membrane  113  used to prepare a collapsible blood container  102  is between 20 and 80%. In another aspect, the void fraction of a multilayered membrane  113  used to prepare a collapsible blood container  102  is between 35 and 50%. 
     In certain aspects, the permeability of multilayered membranes having micropores greater than about 1.0 μm may allow fluid to permeate through the membrane, compromising both the fluid containment and the oxygen and carbon dioxide permeability. To overcome this permeability at high pore sizes, so called “super-hydrophobic” membranes can be employed wherein the contact angle is greater than 150°. As used herein and known in the art, the contact angle quantifies the wettability of a solid surface and is theoretically described by Young&#39;s equation. In certain aspects according the present disclosure, the use of non-hydrophobic multilayered materials is not recommended as the surface tension of the material is lower and allows for fluid to seep through the pores even at the ranges stated above. 
     In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a multilayered permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter. In other aspects, micropores of porous multilayered membranes may be from 0.22 μm to 0.8 μm in diameter. In an aspect, the micropores of porous multilayered membranes are from 0.2 and to 1.0 μm. In another aspect, the micropores of porous multilayered membranes may be greater than 0.1 and less than 1.0 μm. In a further aspect, the micropore of the porous multilayered membrane ranges from about 0.05 μm to about 1.0 μm. In some aspects, the micropores of porous multilayered membranes may be greater than 0.3 or 0.4 μm. In other aspects, the micropores of porous multilayered membranes may be greater than 0.5 or 0.6 μm. 
     In aspects according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a multilayered membrane  113  having a micropore size of less than 1.0 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a multilayered membrane  113  having a micropore size of less than 0.8 μm. In certain aspects according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a multilayered membrane  113  having a micropore size of less than 0.65 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a multilayered membrane  113  having a micropore size of less than 0.45 μm. 
     In an aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a multilayered membrane  113  having a micropore size of 0.1 μm. In another aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a multilayered membrane  113  having a micropore size of 0.22 μm. In another aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a multilayered membrane  113  having a micropore size of 0.20 μm. In a further aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a multilayered membrane  113  having a micropore size of 0.45 μm. In yet a further aspect, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a multilayered membrane  113  having a micropore size of 0.65 μm. In another aspect according to the present disclosure, an oxygen depletion device  10  comprises an inner collapsible blood container  102  comprising a multilayered membrane  113  having a micropore size of 0.8 μm. 
     In aspects according to the present disclosure, the multilayered membrane  113  may be less than 250 μm thick. In certain aspects, the membrane is greater than 10 μm thick. In some aspects the multilayered membrane  113  may be between 10 and 250 μm thick. In other aspects, the multilayered membrane may be between 10 and 125 μm thick or 25 and 150 μm thick. In an aspect, the multilayered membrane  113  may be between 50 and 125 μm thick, 75 and 125 μm thick, 50 and 150 μm thick, 75 and 150 μm thick, 100 and 125 μm thick, 150 and 250 μm thick or between 25 and 150 μm thick, 100 and 125 μm thick, 150 and 250 μm thick or between 25 and 150 μm thick. In another aspect, the membrane  113  of inner collapsible blood container  102  is about 30 μm. In yet another aspect, the membrane  113  of inner collapsible blood container  102  is about 50 μm. In a further aspect, the membrane  113  of inner collapsible blood container  102  is about 76 μm. In an aspect, the membrane  113  of inner collapsible blood container  102  is about 120 μm thick 
     In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a multilayered permeable membrane  113  that is between 100 and 125 μm thick. In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a multilayered permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter and that is between 100 μm and 125 μm thick. In certain aspects according to the present disclosure, the collapsible blood container  102  is prepared from a multilayered permeable membrane  113  having a pore size of between 0.1 μm and 0.8 μm in diameter and that is between 50 μm and 150 μm thick. 
     The present disclosure provides for, and includes, a collapsible blood container  102  that is substantially permeable to oxygen and is a membrane prepared from polyvinyl chloride (PVC). In aspects according the present disclosure, the collapsible blood container  102  can be prepared from a PVC membrane having a thickness of between 5 μm and 250 μm, and more preferably between about 10 μm and about 100 μm. 
     The use of PVC in the manufacture of collapsible blood containers is well known in the art. The use of various plasticizers in various PVC formulations is also well known in the art, and includes the use of diethylhexyl phthalate (DEHP) for long term storage of red blood cells. Typical manufacture of collapsible blood containers from PVC-DEHP utilizes radiofrequency (RF) welding of a pair of films to conveniently fabricate a bag structure, with such individual films having a thickness of about 350 μm to about 400 μm. An exemplary PVC-DEHP film is the Renolit ES-3000 film (American Renolit Corp., City of Commerce, Calif.). 
     Due to the relatively low oxygen permeability of such films and the need for higher oxygen permeability for platelet storage, other plasticizers for PVC have found utility in the fabrication of collapsible blood containers and include the use of citrate, among others (see, for example, “The Role of Poly(Vinyl Chloride) in Healthcare” by Colin R. Blass, copyright 2001 Rapra Technology, Ltd., ISBN:1-85957-258-8). A suitable example of a PVC-citrate film is the Renolit ES-4000 film (American Renolit Corp., City of Commerce, Calif.). 
     The present disclosure provides for suitable PVC materials for use in a collapsible blood container  102  that is substantially permeable to oxygen. The use of a PVC-citrate film such as Renolit ES-4000 having a thickness of from about 5 μm to about 250 μm, and more preferably from about 10 μm to about 100 μm is suitable for providing a collapsible blood container having the desired characteristics of high oxygen permeability, RF welding and joining, and high tensile strength. 
     The present disclosure provides for, and includes, a collapsible blood container  102  that is substantially permeable to oxygen and is a membrane prepared from silicone. In aspects according the present disclosure, the collapsible blood container  102  can be prepared from a silicone membrane having a thickness of between 15 μm and 100 μm. In aspects according the present disclosure, the collapsible blood container  102  can be prepared from a silicone membrane having a thickness of between 5 μm and 500 μm. In other aspects, the collapsible blood container  102  can have a thickness of between 5 μm and 200 μm. In other aspects, the collapsible blood container  102  can have a thickness of between 20 μm and 120 μm. In another aspect the collapsible blood container  102  is between 30 μm and 120 μm thick. In yet another aspect, the collapsible blood container  102  is between 50 μm and 120 μm thick. In a further aspect, the thickness of the collapsible blood container  102  can be between 76 μm and 120 μm. In another aspect the collapsible blood container  102  is between 20 μm and 50 μm thick. The present disclosure provides for, and includes, a collapsible blood container  102  that is 20 μm in thickness. In another aspect, the collapsible blood container  102  is 15 μm thick. In another aspect, the collapsible blood container  102  is 30 μm thick. In yet another aspect, the collapsible blood container  102  is 50 μm thick. In an additional aspect, the collapsible blood container  102  is 120 μm thick. 
     In aspects according the present disclosure, the collapsible blood container  102  can be prepared from a silicone membrane having a thickness of between 20 μm and 400 μm. In other aspects, the collapsible blood container  102  can have a thickness of between 20 μm and 200 μm. In other aspects, the collapsible blood container  102  can have a thickness of between 40 μm and 300 μm. In another aspect, the collapsible blood container  102  is between 40 μm and 400 μm thick. In yet another aspect, the collapsible blood container  102  is between 300 μm and 450 μm thick. In a further aspect, the thickness of the collapsible blood container  102  can be between 350 μm and 450 μm. The present disclosure provides for, and includes, a collapsible blood container  102  that is about 450 μm in thickness. In another aspect, the collapsible blood container  102  is 425 μm thick. In yet another aspect, the collapsible blood container  102  is 400 μm thick. In an additional aspect, the collapsible blood container  102  is 350 μm thick. 
     Suitable silicone membranes include commercially available membranes. Non-limiting examples of silicone membranes are available from Wacker Silicones, such as the Silpuran® brand of medical grade silicone sheet membranes (Wacker Silicones, Adrian, Mich.) and Polymer Sciences PS-1033 P-Derm® silicone elastomer membrane (Polymer Sciences, Inc., Monticello, Ind.). In an aspect, the silicone membrane may be Polymer Sciences PS-1033 or Wacker Silpuran® 6000 silicone. Silicone membranes can be prepared from various liquid silicone rubber (LSR) materials, which are available from a number of silicone suppliers, such as Wacker Silicones (Adrian, Mich.), Shin-Etsu Silicones of America (Akron, Ohio), NuSil Technology (Carpenteria, Calif.), and Blue Star Silicones (East Brunswick, N.J.), to name a few. 
     In an aspect according to the present disclosure, a collapsible blood container  102  can be manufactured from silicone by various molding methods such as compression molding, injection molding, and insert molding, and also adhesive bonding of silicone sheets using silicone adhesives. In one aspect according to the present disclosure, a pair of silicone sheets are bonded together around the periphery with a section of silicone inlet tubing in place in the seam using silicone adhesive. In another aspect according to the present disclosure, a silicone liquid rubber is injection molded over a form to create a three-sided shape, which is then further bonded to closure on the remaining fourth side around a silicone inlet tube using a silicone adhesive. In another aspect according to the present disclosure, a silicone liquid rubber is injection molded over a form to create a three-sided shape, which is then insert molded onto a closure shape on the remaining fourth side that incorporates an inlet tubing into the closure shape. 
     The present disclosure provides for, and includes, a collapsible blood container  102  having resistance to tearing. As used herein, “tear resistance” or “tear strength” is measured in kN/m. In aspects according the present disclosure, the collapsible blood container  102  should be prepared from oxygen permeable materials that are also resistant to tearing. Measures of tear resistance are known in the art, for example, ASTM D-412, which can also be used to measure tensile strength, modulus, and elongations. In certain aspects, collapsible blood container  102  should be prepared from oxygen permeable materials that are resistant to the formation of a tear (e.g., tear initiation). Methods of measuring tear initiation and tear propagation are known in the art, for example ASTM D-624. Other methods include measuring the tensile strength and the elongation at break according to DIN 53 504-S1. 
     In an aspect according to the present disclosure, a collapsible blood container  102  should be prepared from oxygen permeable materials having a tensile strength of at least 2.4 N/mm 2 . 
     The present disclosure provides for, and includes, sorbents capable of binding to and removing oxygen from an environment. Unless provided otherwise, the term “sorbent” refers to oxygen sorbents and scavengers. As used herein, “oxygen scavenger” or “oxygen sorbent” is a material that binds irreversibly to or combines with O 2  under the conditions of use. The term “oxygen sorbent” may be used interchangeably herein with “oxygen scavenger.” In certain aspects according the present disclosure, a material may bind to or combines with oxygen irreversibly. In other aspects, oxygen may bind to a sorbent material and have a very slow rate of release, k off . In an aspect, the oxygen may chemically react with some component of the material and be converted into another compound. Any material where the off-rate of bound oxygen is much less than the residence time of the blood can serve as an oxygen scavenger. 
     As used herein, the amount of sorbent is provided as having a certain binding capacity of oxygen as measured by volume (e.g., cubic centimeters (cc) or milliliters (ml)) at standard temperature and pressure (e.g., 0° C. (273.15 Kelvin) and 1.01×10 5  pa (100 kPa, 1 bar, 0.986 atm, 760 mmHg) of pressure). In other aspects, oxygen sorbents and scavengers are further capable of binding to and removing carbon dioxide from an environment. In certain aspects, sorbent  103  may be a mixture of non-toxic inorganic and/or organic salts and ferrous iron or other materials with high reactivity toward oxygen, carbon dioxide, or oxygen and carbon dioxide. In certain aspects, an oxygen sorbent or scavenger is combined with a carbon dioxide sorbent. In other aspects, the presence or absence of carbon dioxide binding capabilities of an oxygen sorbent is not necessary. 
     Suitable oxygen sorbents or scavengers are known in the art. Suitable oxygen sorbents according to the present disclosure have minimum oxygen adsorption rates of 0.44 ml/min. Sorbents having suitable adsorption profiles bind at least 45 ml O 2  within 60 minutes, 70 ml O 2  within 120 minutes, and 80 ml O 2  within 180 minutes. Suitable sorbents may have both higher capacity and binding rates. 
     Non-limiting examples of oxygen scavengers or sorbents include iron powders and organic compounds. Examples of O 2  sorbents include chelates of cobalt, iron, and Schiff bases. Additional non-limiting examples for O 2  sorbents may be found in U.S. Pat. No. 7,347,887 issued to Bulow et al., U.S. Pat. No. 5,208,335, issued to Ramprasad et al., and U.S. Pat. No. 4,654,053 issued to Sievers et al.; each of which is hereby incorporated by reference in their entireties. Oxygen sorbent materials may be formed into or incorporated in fibers, microfibers, microspheres, microparticles, and foams. 
     In certain aspects, suitable sorbents include those obtainable from Multisorb Technologies (Buffalo, N.Y.), Sorbent Systems/Impak Corporation (Los Angeles, Calif.) or Mitsubishi Gas Chemical America (MGC) (New York, N.Y.). Exemplary oxygen sorbents include Multisorb Technologies StabilOx® packets, Sorbent Systems P/N SF100PK100 100 cc oxygen absorber, and Mitsubishi Gas Chemical America Ageless® SS-200 oxygen absorber. MGC also provides sorbents suitable for the methods and devices of the present disclosure. Such suitable oxygen sorbents include the MGC Ageless® and SS-200 oxygen absorber. 
     In aspects according to the present disclosure, a sorbent may be an oxidizable organic polymer having a polymeric backbone and a plurality of pendant groups. Examples of sorbents with a polymeric backbone include a saturated hydrocarbon (&lt;0.01% carbon-carbon double bonds). In some aspects, the backbone can contain monomers of ethylene or styrene. In an aspect, a polymeric backbone may be ethylenic. In another aspect, an oxidizable organic compound may be ethylene/vinyl cyclohexene copolymer (EVCH). Additional examples of substituted moieties and catalysts are provided in U.S. Patent Publication No. 2003/0183801 by Yang et al., hereby incorporated by reference in its entirety. In additional aspects, an oxidizable organic polymer can also comprise substituted hydrocarbon moieties. Examples of oxygen scavenging polymers include those described by Ching et al., International Patent Publication WO99/48963, hereby incorporated by reference in its entirety. Oxygen scavenging materials may include those provided in U.S. Pat. No. 7,754,798 issued to Ebner et al., U.S. Pat. No. 7,452,601 issued to Ebner et al., or U.S. Pat. No. 6,387,461 issued to Ebner et al., each of which are hereby incorporated by reference in their entireties. 
     As used herein, sorbents of the present disclosure may be either free or contained in a permeable enclosure, container, envelope, etc. In certain aspects, sorbent is provided in one or more sachets made of materials having high porosity and essentially no resistance to the transport of gases. Examples of such materials include spun polyester films, perforated metallic foils, and combinations thereof. 
     The present disclosure further includes, and provides for, sorbent incorporated as one or more laminated layers of an outer article substantially impermeable to oxygen. Polymeric sorbents such as those described above may be laminated to sheets used to prepare an outer receptacle using methods known in the art, including soft contact lamination, thermal lamination, or solvent lamination. 
     The present disclosure further includes, and provides for, sorbents formed inside the pores of porous micro-glass fibers or encapsulated in other inert materials. The encapsulation of transition-metal complexes within the pores of a porous material may be achieved by using a ship-in-a-bottle synthesis in which the final molecule is prepared inside the pores by reacting smaller precursors. Examples of such encapsulated sorbents are known in the art, for example, as described by Kuraoka, et al., “Ship-in-a-bottle synthesis of a cobalt phthalocyanine/porous glass composite membrane for oxygen separation,”  Journal of Membrane Science,  286(1-2):12-14 (2006), herein incorporated by reference in its entirety. In some aspects, porous glass fibers may be manufactured as provided in U.S. Pat. No. 4,748,121 issued to Beaver et al., herein incorporated by reference in its entirety. In another aspect, a sorbent can formed as a porous sheet product using papermaking/non-woven wet-laid equipment. Sheets with O 2  scavenging formulations may be as described in U.S. Pat. No. 4,769,175 issued to Inoue, herein incorporated by reference in its entirety, which can be formed and then encapsulated with a silicone film. 
     As used herein, “carbon dioxide scavenger” is a material that binds to or combines with carbon dioxide under the conditions of use. The term “carbon dioxide sorbent” may be used interchangeably herein with “carbon dioxide scavenger.” In certain aspects, carbon dioxide sorbents may be non-reactive, or minimally reactive with oxygen. In other embodiments, oxygen sorbents may exhibit a secondary functionality of carbon dioxide scavenging. Carbon dioxide scavengers include metal oxides and metal hydroxides. Metal oxides react with water to produce metal hydroxides. The metal hydroxide reacts with carbon dioxide to form water and a metal carbonate. In certain aspects according the present disclosure, a material may bind to or combine with CO 2  irreversibly. In aspects according to the present disclosure, a material may bind CO 2  with higher affinity than hemoglobin. In other aspects, a sorbent material may bind CO 2  with high affinity such that the carbonic acid present in the blood or RBC cytoplasm is released and absorbed by the sorbent. In other aspects, CO 2  binds to a sorbent material and has a very slow rate of release, k off . In an aspect, the carbon dioxide can chemically react with some component of the material and be converted into another compound. 
     Carbon dioxide scavengers are known in the art. In certain aspects according to the present disclosure, a carbon dioxide scavenger may be calcium oxide. Reaction of calcium oxide with water produces calcium hydroxide that may react with carbon dioxide to form calcium carbonate and water. In certain aspects according the present disclosure, the water for the production of calcium hydroxide is obtained via diffusion of blood derived water vapor through the inner oxygen permeable container. In another aspect, the water may be provided by the environment through the outer receptacle that is substantially impermeable to oxygen. In yet another aspect, the water may be included with the outer receptacle of the oxygen depletion device. 
     Non-limiting examples of CO 2  scavengers include oxygen scavengers and carbon dioxide scavengers provided by Multisorb Technologies (Buffalo, N.Y.). Oxygen scavengers may exhibit a secondary functionality of carbon dioxide scavenging. 
     In aspects according to the present disclosure, O 2  depletion media and CO 2  depletion media may be blended to a desired ratio to achieve desired results. 
     The present disclosure further includes and provides for sorbents contained in sachets. As used herein, a “sachet” is any enclosure that encloses and contains an oxygen sorbent, a carbon dioxide sorbent, or a combination of oxygen and carbon dioxide sorbent(s). Sachets according the present disclosure are contained within overwrap material that is both oxygen and carbon dioxide permeable. In certain embodiments, the overwrap material may be a combination of two or more materials, at least one of the materials being oxygen and carbon dioxide permeable. Suitable overwrap materials have a known biocompatible profile or meet ISO 10993. 
     Sachets are sealed so that the sorbent contents are wholly contained within the overwrap material and do not allow the sorbent to leak or otherwise exit its overwrap package. Sachets may take any shape, though typically take a rectangular or square shape. In an aspect, the sachet is about 50×60 mm. In an aspect, the oxygen sorbent  103  binds 30 cc oxygen per sachet at STP. In an aspect, the oxygen sorbent  103  binds 60 cc oxygen per sachet at STP. In an aspect, the oxygen sorbent  103  binds 120 cc oxygen per sachet at STP. In an aspect, the oxygen sorbent  103  binds from 30 to 120 cc oxygen per sachet at STP. In an aspect, the oxygen sorbent  103  binds from 30 to 120 cc oxygen per sachet at STP. In an aspect, the oxygen sorbent  103  binds from 50 to 200 cc oxygen per sachet at STP. In certain aspects according to the present disclosure, a sachet has a total oxygen adsorption capacity of 100 cc O 2  at STP. In certain other aspects of the present disclosure, a sachet has a total oxygen absorption capacity of at least 200 cc O 2  at STP. 
     In aspects according to the present disclosure, the oxygen sorbent  103  may be provided in one or more sachets. In another aspect, the oxygen sorbent  103  is provide in a single larger sachet. In other aspects, the oxygen sorbent  103  is provided in two sachets distributed within the headspace between the inner collapsible container  102  and the outer receptacle  101 . In yet other aspects, the oxygen sorbent  103  is provided in four sachets distributed within the headspace between the inner collapsible container  102  and the outer receptacle  101 . In aspects according to the present disclosure, an oxygen depletion device  10  may comprise 2 to 20 sorbent packages. 
     In aspects according to the present disclosure, oxygen depletion device  10  includes from 1 to 50 grams of sorbent  103  contained in one or more sachets. In an aspect, an oxygen depletion device  10  includes from 1 to 100 grams of sorbent  103  contained in one or more sachets. In an aspect, an oxygen depletion device  10  includes from 25 to 75 grams of sorbent  103  contained in one or more sachets. In a further aspect, an oxygen depletion device  10  includes about 25 grams of sorbent  103 . In yet another aspect, oxygen depletion device  10  includes about 50 grams of sorbent  103 . In an aspect, an oxygen depletion device  10  includes about 35 or 45 grams of sorbent  103  contained in one or more sachets. In an aspect, an oxygen depletion device  10  includes about 10 or 15 grams of sorbent  103  contained in one or more sachets. The sachets can be square, rectangular, circular, or elliptical and have a perimeter of 40 to 150 mm. 
     Sachets according to the present disclosure may further include a carbon dioxide sorbent. In an aspect, an oxygen sorbent  103  also provides for carbon dioxide adsorption. In an aspect, the oxygen sorbent  103  binds 30 cc carbon dioxide at STP. In an aspect, the oxygen sorbent  103  binds at least 170 cc oxygen and at least 30 cc carbon dioxide, where both gases are measured at STP. 
     The present disclosure provides for, and includes, an outer receptacle  101  that is substantially impermeable to oxygen. As discussed above, the integrity of the oxygen barrier should be maintained when joining, welding, folding, or otherwise assembling an outer receptacle  101 . Failures in assembly of the outer receptacle  101  compromises the shelf life of an oxygen depletion device  10  or renders it unable to perform its intended purpose of depleting oxygen from blood. Importantly, blood that is inadequately depleted of oxygen does not realize the benefits of depletion during storage and may have significant negative consequences when transfused into a patient. In addition to satisfying the requirements for blood collection and depletion, it is routine for blood to be sampled through standardized ports  303  as well as for various additives to be introduced into the collected blood. More specifically, nearly all collected blood is provided with an anticoagulant at or during collection. 
     To address the need to introduce materials into the collected blood, and to provide for the transfer of blood that has been depleted of oxygen to an appropriate anaerobic storage bag, an oxygen depletion device  10  may further include one or more inlets/outlets  30 . As provided herein, special care in the assembly of the outer receptacle  101  (and outer receptacle  201 ) is necessary to ensure that when the oxygen impermeable outer receptacle  101  (and outer receptacle  201 ) is traversed, the inlet/outlet  30  does not become a source of unwanted oxygen ingress. 
     In aspects according to the present disclosure, the outer receptacle  101  includes one or more inlets/outlets  30 . In certain aspects, the one or more inlet/outlets  30  further comprise a spike port  303 . 
     It is notable that few materials provide complete impermeability and that even the high impermeability of materials can be compromised when joining, welding, folding, or otherwise assembling an outer receptacle  101 . As will be discussed below, oxygen depletion device  10  may further incorporate optional spike ports  303  and inlets/outlets  30  and must also be designed to accommodate changes in volume of the inner collapsible blood container  102 . Accordingly, special care is taken to incorporate specific design elements and manufacturing methods to ensure the integrity of the impermeable barrier. 
     Spike ports  303  for use in blood collection kits and systems are commonly known in the art and include products such as Vitalmed #20391 (Vitalmed, Inc., Lakeville, Mass.) and Qosina 65842 (Qosina Corp., Edgewood, N.Y.). These ports are typically molded from PVC and have a removable cap that provides for a sterile barrier before use, and also provides for some degree of oxygen impermeability to the contents. In some aspects, a spike port  303  is covered by a sealed, frangible section of the outer receptacle film, thereby providing for a sterile barrier and also providing an additional degree of oxygen impermeability. Improved oxygen impermeability is desirable as it increases the shelf life of kits and systems having an oxygen depletion device  10 . 
     As will be appreciated, conventional ports, inlets, and outlets are potential sources of unwanted oxygen ingression that depend both on the selection of the material and the methods used to bond the port, inlet, or outlet to the outer receptacle  101 . Methods of bonding materials are well known in the art. As provided herein, inlet/outlet  30  comprises a tube  301  joined to the outer receptacle  101  (or outer receptacle  201 ) using bond  302  which creates an oxygen impermeable seal to the outer receptacle  101  (or outer receptacle  201 ). In an aspect, bond  302  is achieved by using constant heat sealing dies heated to and maintained at about 210° F. In an aspect, films are placed between heated dies and clamped together for about to achieve a thermally welded seam. In certain aspects, a heat seal is created in about 5 seconds. In certain aspects, the sealing dies have a grooved section machined out of them to accommodate an intermediary component. In some aspects tube  301  comprises an intermediary component that may be a length of multilayer tubing as discussed below or a small block of machined polymer or molded device. In certain aspects, a molded device is prepared from a polyolefin, such as polyethylene. In aspects according to the present disclosure, the groove is dimensioned about 10% smaller than the features of the component, thereby providing for compression during sealing. 
     In some aspects, an oxygen impermeable bond is comprised of a section of multilayer tube that is heat sealed into the seam of the outer receptacle  101 . In certain aspects, the multilayer tube is comprised of an outer layer of polyethylene, and inner layer of PVC (polyvinyl chloride), and an intermediary layer of EVA (ethyl-vinyl-alcohol) (Pexco, Inc. Athol, Mass.). In some aspects, additional sections of PVC tubing are solvent bonded into the multilayer tube using, for example, cyclohexanone. 
     In some aspects, an inlet/outlet  30  is comprised of a tube  301  prepared from a small diamond shaped block of polyethylene with a hole through the center, such that the diamond shaped block is heat sealed into the seam of the outer receptacle to provide an oxygen impermeable bond  302  while the center through-hole provides for fluid connectivity with the contents. In an aspect, a section of PVC tubing is bonded into the center hole of the diamond shaped block using an oxygen impermeable adhesive capable of bonding to polyethylene, such as Loctite 4310, Masterbond X17, or 3M Scothweld 4693, thereby providing for fluid connectivity through the oxygen impermeable outer receptacle to the contents therein. In other aspects, a multilayered tubing can be bonded to the center hole of the diamond shaped block using methods known in the art. In other aspects, a multilayered tubing can be utilized in place of standard PVC intravenous tubing to provide for enhanced oxygen barrier properties. 
     The users of the collapsible container require convenient filling and removal of the contents, and must be able to empty the contents within 2 minutes per the ISO 3826 standard for blood containers. The outer receptacle can reduce the filling time by constraining the collapsible container and preventing it from expanding. Thus, in some embodiments, the blood storage device is further comprised of an expansion feature to allow for unrestricted filling of the collapsible container. In some embodiments the expansion feature is comprised of a gusseted fold along one or more edges of the outer receptacle. Typically, a fold of about ¼ inch is adequate to provide for expansion of the inner container, and the pleats of the fold are sealed into the seams at the ends. In some embodiments, the expansion feature is comprised of a third panel of barrier film sealed along the bottom of the outer receptacle, providing for a three-dimensional bag. 
     During the development of the oxygen depletion device  10 , it was discovered that the size, shape, and number of chambers of an inner collapsible blood container  102  needed to be controlled in order to obtain suitable depletion kinetics. More particularly, even using highly permeable materials, using standard blood bag configurations proved inadequate and had significantly slower reaction kinetics. Not to be limited by theory, it is hypothesized that deoxygenation is a multistep process including release of dissolved oxygen from hemoglobin, diffusion of the dissolved oxygen within the red blood cell cytoplasm, and diffusion of the dissolved oxygen through the red blood cell membrane. Also not to be limited by theory, it is hypothesized that the high concentration of hemoglobin, having very high affinity for oxygen, greatly decreases the diffusion rate of the dissolved oxygen within the cytoplasm. Similarly, the diffusion of dissolved oxygen once it passes through the plasma membrane to the plasma is further limited by absorption and binding to other red cells. Again, not to be limited by theory, it is hypothesized that an additional diffusion barrier for the dissolved oxygen occurs at the gas permeable membrane where it not only needs to pass through the membrane, but also changes state from the dissolved phase to the gaseous phase. Subsequent diffusion and adsorption by the sorbent occurs in a gaseous state and is maximized by incorporating and maintaining a headspace within the outer receptacle  101 . Accordingly, it is believed that the diffusion of the gaseous oxygen is maximized by maintaining the concentration gradient within the headspace from the surface of the inner collapsible blood container  102  to the oxygen sorbent  103 . Also not to be limited by theory, it is thought that by selecting sorbents that have high absorption kinetics, high binding capacity, and combinations of both, a suitable diffusion gradient for the gaseous oxygen is maintained to drive the rapid kinetics of oxygen depletion in oxygen depletion device  10 . 
     The present disclosure provides for, and includes, an oxygen depletion device  10  for depleting oxygen from blood that comprises an inner collapsible blood container  102  having a surface to volume ratio of between 4.75 centimeters 2 /milliliter (cm 2 /ml) and 6.9 cm 2 /ml enclosed within an outer receptacle  101 . In certain aspects, an oxygen depletion device  10  for depleting oxygen from blood comprises an inner collapsible blood container  102  having a surface to volume ratio of between 4.84 cm 2 /ml and 6.9 cm 2 /ml enclosed within an outer receptacle  101  when filled with blood for oxygen depletion. In certain aspects, an oxygen depletion device  10  for depleting oxygen from blood comprises an inner collapsible blood container  102  having a surface to volume ratio of between 5.0 cm 2 /ml and 6.9 cm 2 /ml enclosed within an outer receptacle  101  when filled with blood for oxygen depletion. In some aspects, an oxygen depletion device  10  for depleting oxygen from blood comprises an inner collapsible blood container  102  having a surface to volume ratio of between 5.0 cm 2 /ml and 6.5 cm 2 /ml enclosed within an outer receptacle  101  when filled with blood for oxygen depletion. In some aspects, an oxygen depletion device  10  for depleting oxygen from blood comprises an inner collapsible blood container  102  having a surface to volume ratio of between 5.5 cm 2 /ml and 6.5 cm 2 /ml enclosed within an outer receptacle  101  when filled with blood for oxygen depletion. 
     As used herein, surface to volume and surface area to volume are used interchangeably throughout the present disclosure. A used herein, surface to volume ratios are defined with respect to a standard unit of whole blood, about 1 pint or 450-500 ml. As is evident to a person of skill in the art, collection of less than a unit of blood results in an even higher surface to volume ratio and the oxygen depletion device  10  is suitable for collecting a fraction of a unit of blood without modification. For the collection of more than a unit of blood, the size of the collapsible blood container  102  would need to be adjusted to provide for the desirable rapid kinetics of blood depletion. Modifications of the sort necessary to adapt an oxygen depletion device  10  for the collection of more than a unit of blood is within the level of ordinary skill in the art. 
     The present disclosure further includes and provides for oxygen depletion device  10  for the collection and depletion of packed red blood cells. A full unit of packed red blood cells in an additive solution comprises about 280±60 ml. 
     In an aspect according to the present disclosure, the surface to volume ratio of a collapsible blood container  102  is at least 4.84 centimeters/milliliter (cm 2 /ml) when filled with blood for oxygen depletion. Not to be limited by theory, it is believed that by increasing the surface to volume ratio, the diffusion limitations imposed by blood itself, particularly by the red blood cells and hemoglobin, can be overcome by decreasing the diffusion distance of the dissolved oxygen within the inner collapsible blood container  102 . In an aspect, the surface to volume ratio of a blood container  102  is at least 5.0 cm 2 /ml when filled with blood for oxygen depletion. In another aspect, the surface to volume ratio of a collapsible blood container  102  is at least 5.5 cm 2 /ml when filled with blood for oxygen depletion. In a further aspect, the surface to volume ratio of a collapsible blood container  102  is at least 6.0 cm 2 /ml when filled with blood for oxygen depletion. In some aspects, the surface to volume ratio of a collapsible blood container  102  is at least 6.5 cm 2 /ml when filled with blood for oxygen depletion. 
     The present disclosure also includes and provides for increasing the kinetics of deoxygenation of blood by modifying the dimensions of the inner collapsible blood container  102 . Not to be limited by theory, the average diffusion distance of a red blood cell in blood minimized as the height is decreased leading to increased deoxygenation kinetics. In certain aspects according the present disclosure, the collapsible blood container  102  is 25.4 cm by 30.5 cm by 0.02 cm before filling with blood, and about 1.5 cm in height after filling with blood. In other aspects according the present disclosure, the collapsible blood container  102  is 17.5 cm by 28.0 cm (7×11 inches) by 0.04 cm before filling with blood, and about 2.0 cm in height after filling with blood. In other aspects according the present disclosure, the collapsible blood container  102  is 25.0 cm by 60.0 (10×23 inches) cm by 0.04 cm before filling with blood, and about 0.3 cm in height after filling with blood. 
     In certain aspects, the height of a collapsible blood container  102  is no greater than 0.005 cm when empty. In an aspect the height of a collapsible blood container  102  is no greater than 0.1 cm. In certain aspects, the height of a collapsible blood container  102  is between 0.002 and 0.1 cm. When filled with blood, the height of a collapsible blood container  102  is no greater than 0.3 cm. In an aspect the height of a collapsible blood container  102  when filled with blood is no greater than 1.5 cm. In certain aspects, the height of a collapsible blood container  102  when filled with blood is between 0.2 cm and 2.5 cm. 
     The present disclosure also includes and provides for an oxygen depletion device  10  having dimensions suitable for incorporation of existing blood collection protocols using existing equipment. Design of an oxygen depletion device  10  with recognition to existing technologies reduces capital costs in centralized processing centers and further provides for increased consistency and reliability. As used herein, the dimensions of an oxygen depletion device  10  is primarily limited to the length and width of the outer receptacle  101  where the height of the bag is determined by the requirements of the collapsible blood container  102  to contain about a pint or 450 to 500 ml of whole blood, which is equivalent to a “unit of blood”. In another aspect, the dimensions of a collapsible blood container  102  are provided to contain about 220 to 380 ml of packed red blood cells, which is equivalent to a unit of packed red blood cells. The height of an oxygen depletion device  10  is further constrained by the presence of one or more sorbent packets and devices included to maintain an appropriate headspace. In view of these considerations, it become apparent that constraints on the dimension of the outer receptacle  101  of an oxygen depletion device  10  necessarily limits the dimensions of a collapsible blood container  102 . Accordingly, a collapsible blood container  102  may be divided into one or more chambers in fluid communication with each other. 
     In aspects according to the present disclosure, an oxygen depletion device  10  is designed to be incorporated into existing blood agitation equipment. In certain aspects, an oxygen depletion device  10  is dimensioned to efficiently utilize the space available in agitator and mixing tables. In an aspect, an oxygen depletion device  10  is dimensioned to maximally utilize the area available in a platelet agitator, for example a Helmer Labs Platelet Agitator, Model PF96. Suitable dimensions of an oxygen depletion device  10  include those that allow for 1, 2, 4, 6, 8, 10 or more bags to be placed on a flat agitator or mixer surface. 
     The area of a collapsible blood container  102 , within an oxygen depletion device  10  has an area of between about 900 to 1800 cm 2 . Accordingly, an oxygen depletion device  10  that further comprises a spacer  110  effectively doubles the surface area available for gas exchange. In the absence of a spacer  110 , the exchange rate of membrane  113  of the collapsible blood container  102  on the lower surface is significantly reduced and the permeable membrane is contacted by the impermeable film. 
     The present disclosure provides for, and includes, collapsible blood containers  102  further comprising a tie layer  105 , for example as illustrated in  FIGS.  1 A,  1 C,  6 ,  7 ,  9 A,  9 B,  10  and  11   . As used herein a tie layer  105  comprises an intermediate material that bonds (joins) the membranes  113  ( 114 ) together. In certain aspects, the tie layer  105  comprises a solid material having a defined shape. As discussed below, tie layers having a defined shape provide for the incorporation of geometric features  121  including rounded corners and other mixing enhancing shapes. In certain aspects, a tie layer  105  comprises a liquid or gel that can be dried or cured to provide a joining bond between the membranes  113 . Accordingly, a collapsible blood container  102  comprising a silicone membrane  113  can be joined by a liquid silicone tie layer  105 . In certain aspects, the silicone rubber tie layer  116  can be liquid silicone rubber (LSR). 
     The present disclosure provides for, and includes, collapsible blood containers  102  further comprising a tie layer  105  prepared from a solid material that has a lower melting point than the membranes  113 . By providing a tie layer  105  having a lower melting temperature, the membranes  113  can be heat joined via the tie layer  105  without damaging the structure of the microporous membranes, including melting and/or crystallization. In an aspect, the tie layer  105  is selected to have a melting temperature at least 3° C. below the melting temperature of the microporous membranes  113 . In other aspect, the tie layer  105  has a melting temperature at least 10° C. below the melting temperature of the microporous membranes  113 . In other aspects, a suitable tie layer  105  is selected to maximize the difference in temperature between the tie layer and the microporous membranes  113  ( 114 ) to be joined. 
     In aspects according to the present disclosure, the tie layer  105  is selected from LDPE and the microporous membrane  113  is selected from the group consisting of polysulfone, hydrophobic polyvinylidene fluoride (PVDF), cellulose ester, mixed esters of cellulose (MCE), polyethersulfone (PES), polypropylene rendered hydrophobic, and polyacrylonitrile. In an aspect, the tie layer  105  is LDPE and the microporous membrane  113  is polysulfone or hydrophobic polyvinylidene fluoride (PVDF). The present disclosure provides for and includes the selection of suitable microporous membranes as provided herein, and further includes multilayered membranes  113  as provided herein. 
     The present disclosure provides for and includes, construction of collapsible blood containers  102  having a tie layer wherein the tie layer extends beyond the seal indicated as gap  109 , for example as illustrated in  FIG.  9 B . 
     The present disclosure provides for and includes a gap  109  of space between where the seal ends and the tie layer ends. In certain aspects, gap  109  is between 0.05 and 2.5 cm. In other aspects, gap  109  is at least 0.1 cm wide. In other aspects, gap  109  is at least 0.5 cm wide. In other aspects, gap  109  is at least 1 cm wide. In other aspects, gap  109  is at least 1.5 cm wide. In some aspects, gap  109  is between 0.5 and 1.5 cm wide. In other aspects, gap  109  is at least 2 cm wide. In other aspects, gap  109  is between 2 and 2.5 cm wide. In other aspects, gap  109  is at least 2.5 cm wide. 
     As shown in  FIG.  9 B , seals  107  are laminated to the membranes  113  and are in turn laminated to each other as seal  108 . As illustrated in  FIG.  7   , the lamination of the tie layer  105  can be accomplished in two steps, first to the separate membranes  113 , then a second step to join the prelaminated membranes  113  together. In the alternative, the lamination steps can be combined into a single step wherein a single tie layer  105  is used to join membranes together. 
     As shown in  FIG.  9 B , seal  107  may extend beyond the width of seal  108 . By extending seal  107  beyond the width of seal  108 , seal  107  provides for strengthening of flexure point  115 , as indicated in  FIG.  9 A . Without being limited to a specific mechanism, it is believed that the tie layer  105  acts as a reinforcing strain relief inboard of the seal and allows for flexure of the bag at the seal as it is filled and drained of blood product. 
     The present disclosure provides for, and includes, collapsible blood containers  102  having geometric features that improve the mixing of blood during the deoxygenation process. The improved geometries of the present disclosure further include geometries to enhance the filling and draining of the collapsible blood containers  102 . Improved geometries reduce or eliminate ‘dead’ spots in the bag. Not to be limited by theory, dead spot arise in the corners of bags with square geometries. Prior to the present disclosure, methods and blood depletion devices were not time limited and the gas exchange methods typically employed resulted in sufficient mixing. Accordingly, the deficiencies of earlier designs were not revealed. 
     In aspects according to the present disclosure, a collapsible blood containers  102  includes one or more geometric features  121 . In an aspect, the geometric features comprise rounded corners in the collapsible blood container  102  and provide for the elimination of ‘dead’ spots during mixing. The present disclosure provides for the geometric features  121  to be incorporated directly into a tie layer  105 . In other aspects, the geometric features  121  can be incorporated into the collapsible blood container  102  using an external die or plate. In other aspects the geometric features of the collapsible blood container  102  can be provided by a suitable mold having the shape of the geometric feature  121 . In certain aspects, the geometric feature  121  provides a round or oval shape to a collapsible blood container  102 , for example as shown in  FIG.  10   . 
     In certain aspects, the geometric feature  121  can be an ellipse with a first radius from about 0.1 cm to about 7.6 cm and a second radius from about 1 cm to about 7.6 cm. In an aspect, the geometric feature  121  can be an ellipse with a first radius of about 2.5 cm and a second radius of about 5 cm. In an aspect, the geometric feature  121  can be an ellipse with a first radius of about 5 cm and a second radius of about 7.6 cm. In an aspect the geometric feature  121  can be a circle with a diameter of about 5 cm. In an aspect the geometric feature  121  can be a circle with a diameter of about 7.6 cm. 
     As is evident, an oxygen depletion device  10  having a defined size necessarily constrains the dimensions of a collapsible blood container  102  according to the present disclosure. In certain aspects, a collapsible blood container  102  is further limited by a specified surface to volume ratio. In accordance with these limitations, the present disclosure provides for, and includes, a collapsible blood container  102  having two or more chambers in fluid communication with each other. 
     The oxygen depletion container device can be constructed in such a manner that allows for the blood volume to area of bag to be optimized against the overall size of the oxygen depletion container device, while exposing more of the blood volume to the material with oxygen permeability in the utilized space. The blood volume can be contained in a collapsible blood container  102  having two or more chambers that allow for their specific arrangement within the outer receptacle  101 . In certain aspects, the oxygen depletion device  10  height, when placed onto a surface, does not occupy impractical space in the intended mixing apparatus. The chambers can be arranged side to side, stacked on top of one another, partially stacked onto each other, staggered in a row, or saddled on top of each other onto one or more stacking heights. Sorbent  103  can be positioned over or between chambers as needed. Chambers may be filled and drained individually or in unison when such chambers are connected via tubing or fluid conduits that allow for easy filling and draining. It would be understood that the arrangement and interconnection of collapsible blood containers  102  having two or more chambers can be performed by a person of skill in the art. 
     In certain aspects, a collapsible blood container  102  comprises two or more chambers. In an aspect, a collapsible blood container  102  can have two chambers placed side by side or end to end depending on the dimensions. In another aspect, a collapsible blood container  102  can have three chambers placed side by side or end to end depending on the dimensions. In yet another aspect, a collapsible blood container  102  can have three chambers placed side by side or end to end depending on the dimensions. A person of ordinary skill could prepare additional configurations of a collapsible blood container  102  having multiple chambers placed in adjacent positions and orientations to maximize the utilization of space. 
     In other aspects provided for and included in the present disclosure, a collapsible blood container  102  may comprise two or more chambers that are stacked. When in a stacked configuration, to maintain optimal gas diffusion rates, spacers  110  or meshes  110  are included to ensure the separation of adjacent chambers. In certain aspects, the space between a stacked chamber further includes one or more sorbent sachets in order to maintain optimal gas diffusion rates. In certain aspects, two chambers may be stacked. In another aspect, three chambers may be stacked. In yet another aspect, four chambers may be stacked. 
     The present disclosure provides for, and includes, a collapsible blood container  102  comprising a combination of stacked and adjacent chambers. As provided herein, the number and stacking of chambers of a collapsible blood container  102  further comprises a surface to volume ratio of the combined chambers of at least 0.4 cm 2 /ml. Additional variations consistent with the present disclosure can be prepared by one of ordinary skill in the art. 
     The present disclosure provides for, and includes, an oxygen depletion device  10  for depleting oxygen from blood comprising an outer receptacle  101  substantially impermeable to oxygen, inner collapsible blood container  102  that is permeable to oxygen and an oxygen sorbent situated within said outer receptacle wherein the collapsible blood container  102  further comprises one or more mixing structures  119  that increase mixing of the blood during oxygen depletion. In certain aspects, the mixing structures  119  are incorporated into the structure of the collapsible blood container  102 . In other aspects, the mixing structures  119  are added to the inside of, but not physically joined to the collapsible blood container  102 . In yet other aspects, a mixing structure  119  is a structure outside of the collapsible blood container  102  that restricts or modifies the shape of the container  102  to decrease or disrupt laminar flow. Mixing structures  119  according to the present disclosure are designed to increase blood movement in the collapsible blood container  102 , increase turbulent flow within the collapsible blood container  102 , or combinations of both. Importantly, mixing structures and mixing should not significantly increase lysis, or damage to, the red blood cells. 
     In aspects according to the present disclosure, a mixing structure  119  is included in the structure of membrane  113 . In certain aspects, a mixing structure  119  in membrane  113  comprises ridges, bumps, or protrusions on the inside of the collapsible blood container  102  and are in contact with the blood. In an aspect, a mixing structure  119  in membrane  113  comprises one or more ridges. In an aspect, a mixing structure  119  comprises joining the upper and lower membranes  113  ( 114 ) together, for example as illustrated in  FIGS.  10 C and  10 D . In an aspect, the one or more ridges extend across the full width or length of the inner surface of collapsible blood container  102 . In other aspects, the ridges alternate and may be staggered. In certain aspects, the mixing structure  119  in membrane  113  comprises bumps or other protrusions designed to disrupt laminar flow and induce turbulence. Similarly, in certain aspects, the mixing structure  119  in membrane  113  comprises depressions designed to disrupt laminar flow and induce turbulence. In certain aspects, the mixing structures  119  are baffles incorporated into membrane  113 . Baffles are flow directing vanes or panels. In some aspects, a mixing structure  119  comprising one or more baffles may be incorporated into a second membrane  114 . 
     In certain aspects, a mixing structure  119  is contained within the collapsible blood container  102 . In an aspect, a mixing structure  119  within the collapsible blood container  102  comprises one or more beads or balls that aid in mixing when the collapsible blood container  102  is agitated. In another aspect, a mixing structure  119  within the collapsible blood container  102  comprises one or more strings or elongated structures that aid in mixing when the collapsible blood container  102  is agitated. In yet another aspect, a mixing structure  119  within the collapsible blood container  102  comprises a mesh that aids in mixing when the collapsible blood container  102  is agitated. 
     The present disclosure provides for, and includes, an oxygen depletion device  10  having an outer receptacle  101  that is substantially impermeable to oxygen enclosing an inner collapsible blood container  102  and providing a headspace. In an aspect, the oxygen sorbent  103  is disposed within the headspace thereby creating an oxygen depleted state within the headspace. In an aspect, said oxygen sorbent  103  disposed in the headspace further maintains the headspace in an oxygen depleted state by removing oxygen that may enter through the outer receptacle  101  or through the one or more inlets/outlets  30 . 
     Maintaining the headspace in an oxygen depleted state provides for improved shelf life for oxygen depletion device  10 . In an aspect, an assembled oxygen depletion device  10  has a shelf life of at least 24 months. In another aspect, the oxygen depletion device  10  has a shelf life of at least 12 months after assembly of the components. In an aspect according to the present disclosure, the assembled oxygen depletion device  10  meets ISTA-2A standards. 
     In certain aspects of the present disclosure, the headspace provides for improved processing times. For oxygen depletion device  10 , removing ambient air present or inert flushing gas from the assembly prior to sealing the outer receptacle  101  reduces the volume of the headspace. Applying a vacuum to the outer receptacle  101  prior to sealing reduces the volume of the headspace and decreases the total volume of the assembled oxygen depletion device. While reduced overall headspace volume provides for reduced shipping volume, it can result in increased filling times by constraining the collapsible blood container  102 . In certain aspects, the headspace may be flushed with nitrogen gas and then sealed under slightly less than ambient pressure to provide a reduced headspace volume in the oxygen depletion device  10  without significantly increasing the fill and process time. 
     In certain aspects, the headspace may be initially depleted of oxygen by flushing the headspace with nitrogen. In an aspect, the headspace of oxygen depletion device  10  is flushed with nitrogen gas prior to sealing the outer receptacle  101 . In an aspect, the flushing gas is ≥99.9% nitrogen gas. 
     The present disclosure includes and provides for oxygen depletion device  10  having inner collapsible blood container  102  divided into two or more compartments. In certain aspects, an oxygen depletion device  10 , having a collapsible blood container  102  divided into multiple compartments has a headspace of between 10 and 500 ml per compartment. In an aspect the headspace is between 20 and 400 ml per compartment. In another aspect the headspace volume is between 60 and 300 ml per compartment. In a further aspect, the headspace volume is between 100 and 200 ml per compartment of a collapsible blood container. In an aspect, an oxygen depletion device  10  having inner collapsible blood container  102  divided into compartments has a headspace of about 10 ml per compartment. In another aspect, the headspace is about 100 ml to about 200 ml per compartment. In another aspect the headspace is about 300 ml to about 500 ml per compartment. 
     The present disclosure includes and provides for oxygen depletion device  10  having inner collapsible blood container  102  divided into two or more compartments. In certain aspects, an oxygen depletion device  10 , having a collapsible blood container  102  divided into two compartments has a headspace of between 20 and 1000 ml. In an aspect the headspace is between 100 and 800 ml. In another aspect the headspace volume is between 200 and 700 ml. In a further aspect, the headspace volume is between 300 and 500 ml for a two compartment collapsible blood container. In an aspect, an oxygen depletion device  10  having inner collapsible blood container  102  divided into two compartments has a headspace of about 700 ml. In another aspect, the headspace is about 200 ml to about 700 ml. In another aspect the headspace is about 300 ml to about 500 ml. 
     The present disclosure includes and provides for oxygen depletion device  10  having inner collapsible blood container  102  divided into two or more compartments. In certain aspects, an oxygen depletion device  10 , having a collapsible blood container  102  divided into three compartments has a headspace of between 20 and 1000 ml. In an aspect the headspace is between 100 and 800 ml. In another aspect the headspace volume is between 200 and 700 ml. In a further aspect, the headspace volume is between 400 and 600 ml for a three compartment collapsible blood container. In an aspect, an oxygen depletion device  10  having inner collapsible blood container  102  divided into three compartments has a headspace of about 800 ml. In another aspect, the headspace is about 200 ml to about 700 ml. In another aspect the headspace is about 400 ml to about 600 ml. In an aspect the headspace is about 7000 ml due to full expansion of the headspace area. In another aspect the headspace is between 700 and 7000 ml. In another aspect the headspace is between 800 and 6000 ml. In another aspect the headspace is between 1000 and 5000 ml. In another aspect the headspace is between 2000 and 4000 ml. 
     The present disclosure includes and provides for an oxygen depletion device  10  having an inner collapsible blood container  102  and further including one or more spacers  110  that ensure the separation of the outer receptacle  101  and the inner collapsible blood container  102 . The spacer  110  provides for the maintenance of the headspace in the oxygen depletion device to ensure efficient diffusion of the oxygen from the surface of membrane  113  to the sorbent  103 . A spacer  110  can be prepared from one or more of the materials selected from the group consisting of a mesh, a molded mat, a woven mat, a non-woven mat, a strand veil, and a strand mat. In certain aspects, the spacer  110  can be integrated directly into the collapsible blood container  102  as ribs, dimples, or other raised feature that maintains a separation between the outer receptacle  101  and the inner collapsible blood container  102 . The present specification also includes and provides for a spacer  110  to be integrated into the outer receptacle  101  as ribs, dimples, or other suitable raised feature capable of maintaining a separation between the outer receptacle  101  and the inner collapsible blood container  102 . Mixing is an important aspect of the present disclosure. In one aspect of the present disclosure, spacer  110  is selected to be flexible so as to not interfere with the flow of the blood product. 
     The present disclosure includes, and provides for, a spacer  110  having open areas, for the free diffusion of gas from the surface of the permeable membranes  113  and  114 . In an aspect, the spacer  110  is provided as a mesh  110  having open spaces  111 . As used herein, the open area  111  is also referred to as the interstice  111 . As provided herein, the interstice  111  may be provided by a regular weave of a mesh  110 , such that the interstice  111  is regular and repeating within the spacer  110 . In other aspects, the interstice  111  may comprise an irregular open area, for example as provided by a spacer  110  constructed from a non-woven mesh. In an aspect, the interstice  111  has an area of between about 0.5 milimeters 2  (mm 2 ) and about 100 mm 2 . In a further aspect, the interstice  111  has an area of between 1 mm 2  and 10 mm 2 . In other aspects the interstice  111  has an opening that is greater than 0.75 mm 2  per opening. In an aspect the open area or interstitial space of a mesh comprise between 30% to 90% of the total area of a spacer  110 . In an aspect the open area or interstitial space of a mesh comprise between 50% to 80% of the total area of a spacer  110 . In a further aspect, the open area comprises about 60%. In other aspects, the open area comprises up to 75% of the total area. 
     The present disclosure provides for, and includes, inner collapsible blood containers  102  having a spacer  110  incorporated into the membrane  113 , the membrane  114 , or both. In aspects according to the present disclosure, the spacer  110  provides for both the separation of the outer receptacle  101  and the inner collapsible blood container  102  but also for the reinforcement of the permeable membranes. In aspects according to the present disclosure, the spacer  110  prevents tearing, puncturing and bursting of the inner collapsible blood container  102  when filled with blood and used in the depletion methods of the present disclosure. In some aspects, the spacer  110  is provided as a mesh  110  that is integrated into a silicone membrane during the manufacturing process. In other aspects, the spacer  110  is applied to, and joined to, a finished silicone membrane. In other aspects, the spacer  110  is provided as an integrated mesh of a porous membrane. 
     In an aspect, a membrane  113  or  114  having an integrated spacer  110  is prepared from a suspension of liquid silicone rubber (LSR). In an aspect, the LSR is suspended in xylene, hexane, tert butyl acetate, heptane, acetone, or naptha. In aspects according to the present disclosure, the suspension comprises 10 to 30% LSR. As provided herein, a membrane  113  or  114  having an integrated spacer  110  is prepared by providing a 20 to 750 μm layer of an LSR suspension, partially curing the LSR layer and applying a spacer  110  as provided in the present disclosure and performing a second curing step to provide a cured 10 to 100 μm thick silicone membrane  113  having an integrated spacer  110 . 
     The present disclosure also includes and provides for a mesh  110  comprising co-extruded fibers having an inner material  117  and binding material  118 . In aspects according to the present disclosure, binding material  118  is integrated into the pores of membrane  113  ( 114 ) during application of the mesh  110  to the membrane. In an aspect, the binding material  118  is integrated into the pores of a porous membrane  113  by heating. In aspects according to the present disclosure, binding material  118  may be selected from the group consisting of ethylvinyl alcohol (EVOH), ethylvinylacetate (EVA), or acrylate. In aspects according to the present disclosure co-extruded fibers having an inner material  117  and binding material  118  are meshes  110  that include the DuPont Bynel® series of modified ethyl vinyl acetates and modified ethyl vinyl acrylates. 
     The present disclosure also includes and provides for inner collapsible blood containers  102  that further comprise a window  112 . As used herein, a window  112  is made of a transparent material and is bonded or otherwise incorporated into the inner collapsible blood container  102 . In accordance with the present disclosure, suitable materials for window  112  are blood compatible. In certain aspects, materials suitable for a window  112  are oxygen impermeable. In other aspects, materials suitable for a window  112  are oxygen impermeable. The size of a window  112  need only be large enough to provide observation of the blood. 
     Also included and provided for by the present disclosure are collapsible blood containers having bis(2-ethylhexyl) phthalate (DEHP). DEHP is included in most PVC based blood storage bags as a plasticizer where it has been observed that DEHP provides a protective effect to stored red blood cells. See U.S. Pat. No. 4,386,069 issued to Estep. In certain aspects, an oxygen depletion device  10  may further include DEHP incorporated in the inner collapsible blood container  102 . In other aspects, DEHP may be provided separately within the inner collapsible blood container  102 . 
     The present disclosure provides for, and includes, an oxygen depletion device  10  that does not include DEHP. It has been hypothesized that DEHP may act as an endocrine disruptor and certain regulatory agencies are considering ordering the removal of DEHP from blood bags. It has been observed that DEHP may not be necessary when red blood cells are stored anaerobically. See, International Patent Publication No. WO 2014/134503, hereby incorporated by reference in its entirety. Accordingly, in certain aspects, oxygen depletion device  10  entirely excludes DEHP from all blood contacting surfaces. In other aspects, oxygen depletion device  10  limits DEHP containing surfaces to tubing, ports, and inlets such as those illustrated in the Figures at, for example,  106  and  205 . In an aspect, oxygen depletion device  10  excludes a DEHP containing collapsible blood container  102 . 
     The present disclosure provides for, and includes, an oxygen depletion device  10  having an oxygen indicator  104 . Similarly, the present disclosure provides for, and includes, blood storage device  20  having an oxygen indicator  206 . In an aspect, the oxygen indicator  206  detects oxygen and indicates that the oxygen depletion device  10  has been compromised and is no longer suitable for its intended purpose. In an aspect, the oxygen indicator  206  provides a visual indication of the presence of oxygen. In certain aspects, the oxygen indicator  206  provides an indication of the amount of oxygen. 
     In an aspect according to the present disclosure, the outer receptacle may contain an oxygen indicator to notify the user if the oxygen sorbent is no longer active for any reason, such as age, or if the outer receptacle has been compromised, allowing excess oxygen to ingress from ambient air. Such oxygen indicators are readily available and are based on a methylene blue indicator dye that turns blue in the presence of oxygen of about 0.5% or more and pink when the oxygen level is below about 0.1%. Examples of these oxygen indicators are the Tell-Tab oxygen indicating tablet from Sorbent Systems, Inc. (Impak Corp., Los Angeles, Calif.), and the Oxygen Indicator tablet from Mitsubishi Gas Chemical America (MGCA, NY, N.Y.). 
     The present disclosure provides for, and includes, methods for preparing blood for storage under oxygen depleted conditions comprising providing blood having red blood cells having oxygen to be removed to an oxygen depletion device  10 , incubating the blood for a period of time, and transferring the deoxygenated blood to an anaerobic storage bag. In aspects according to the present disclosure, the method further includes agitating the oxygen depletion device  10  to provide for mixing of the blood for deoxygenation. In other aspects, due to the configuration of the oxygen depletion device  10 , agitating is not necessary. 
     For safety, the collection and processing of blood is regulated by a national or regional governmental agency. In the U.S., the Food and Drug Administration (FDA) has established guidelines for the proper handling of blood and blood products. Similarly in Europe, the European Union has been granted regulatory authority that is binding for member states, and typically follows guidelines provided by the Council of Europe. The key requirements for blood establishments and for hospital blood banks in the United Kingdom (UK), for example, are defined in the Blood Safety and Quality Regulations (Statutory Instrument 2005 No. 50) and are enforced by the Medicines and Healthcare products Regulatory Agency, whose powers are derived from UK legislation, to maintain the safety and quality of blood and blood products for transfusion within the UK. 
     Generally, the guidelines established by the various authorities fall in to two main groups. In the first group, as exemplified by the U.S., the allowable time period from donor collection to processing for platelets, and thus driving the storage of RBC&#39;s at 2 to 6° C., is 8 hours. That is, the various processing steps, currently including plasma separation and collection, leukoreduction, platelet separation and collection and packed red blood cell preparation, need to be completed, and various components stored within 8 hours in order to preserve the viability of the platelets (see Moroff &amp; Holme, “ Concepts about current conditions for the preparation and storage of platelets” in Transfus Med Rev  1991; 5: 48-59). In Europe, the period available for processing is 24 hours. Accordingly the methods and processes provided in the present disclosure are designed to achieve the beneficial, and storage lesion reducing, level of deoxygenation for blood storage within about 8 hours from venipuncture. 
     In accordance with the methods of the present disclosure, blood may be obtained from a donor and processed to less than 20% oxygen saturation within 12 hours of collection. Beginning the depletion process, at or soon after collection, improves the efficiency of the process by leveraging the increases in reaction rates due to the higher temperatures. In an aspect, the blood is collected from a donor at about 37° C. and collected in an oxygen depletion device  10  having a suitable amount of anticoagulant. In addition to the increased temperature, the whole blood is typically about 35-65% oxygen saturated when collected by venipuncture from a patient. In one aspect of the present disclosure the whole blood is 35-65% oxygen saturated when collected by venipuncture from a patient. In another aspect the whole blood is 40-60% oxygen saturated when collected by venipuncture from a patient. In another aspect the whole blood is 45-55% oxygen saturated when collected by venipuncture from a patient. In another aspect the whole blood is 50-65% oxygen saturated when collected by venipuncture from a patient. Conventional methods do not provide collection kits and bags that prevent the ingress of oxygen. Thus delays in beginning the oxygen reduction process can greatly increase the time necessary to prepare oxygen-reduced blood having less than 20% oxygen saturation. 
     The methods and devices of the present disclosure further provide for the preparation of oxygen-reduced blood having less than 10% oxygen saturation. In an aspect, the 10% level is achieved within 8 hours or less of collection from a donor. In other aspects, the blood is reduced to less than 10% oxygen saturation in 6 hours or less. In yet other aspects, the blood is reduced to less than 10% oxygen saturation in 4 hours or less. 
     As used herein, the term “blood” refers to whole blood, leukoreduced RBCs, platelet reduced RBCs, and leukocyte and platelet reduced RBCs. The term blood further includes packed red blood cells, platelet reduced packed red blood cells, leukocyte reduced packed red blood cells (LRpRBC), and leukocyte and platelet reduced packed red blood cells. The temperature of blood can vary depending on the stage of the collection process, starting at the normal body temperature of 37° C. at the time and point of collection, but decreasing rapidly to about 30° C. as soon as the blood leaves the patient&#39;s body and further thereafter to room temperature in about 6 hours when untreated, and ultimately being refrigerated at between about 2° C. and 6° C. 
     As used herein, the term “whole blood” refers to a suspension of blood cells that contains red blood cells (RBCs), white blood cells (WBCs), platelets suspended in plasma, and includes electrolytes, hormones, vitamins, antibodies, etc. In whole blood, white blood cells are normally present in the range between 4.5 and 11.0×10 9  cells/L and the normal RBC range at sea level is 4.6-6.2×10 12 /L for men and 4.2-5.4×10 12 /L for women. The normal hematocrit, or percent packed cell volume, is about 40-54% for men and about 38-47% for women. The platelet count is normally 150-450×10 9 /L for both men and women. Whole blood is collected from a blood donor, and is usually combined with an anticoagulant. Whole blood, when collected is initially at about 37° C. and rapidly cools to about 30° C. during and shortly after collection, but slowly cools to ambient temperature over about 6 hours. Whole blood may be processed according to methods of the present disclosure at collection, beginning at 30-37° C., or at room temperature (typically about 25° C.). As used herein, a “unit” of blood is about 450-500 ml including anticoagulant. 
     As used herein, a “blood donor” refers to a healthy individual from whom whole blood is collected, usually by phlebotomy or venipuncture, where the donated blood is processed and held in a blood bank for later use to be ultimately used by a recipient different from the donor. A blood donor may be a subject scheduled for surgery or other treatment that may donate blood for themselves in a process known as autologous blood donation. Alternatively and most commonly, blood is donated for use by another in a process known as heterologous transfusion. The collection of a whole blood sample drawn from a donor, or in the case of an autologous transfusion from a patient, may be accomplished by techniques known in the art, such as through donation or apheresis. Whole blood obtained from a donor using venipuncture has an oxygen saturation ranging from about 30% to about 70% saturated oxygen (sO 2 ). 
     As used herein, “red blood cells” (RBCs) includes RBCs present in whole blood, leukoreduced RBCs, platelet reduced RBCs, and leukocyte and platelet reduced RBCs. Human red blood cells in vivo are in a dynamic state. The red blood cells contain hemoglobin, the iron-containing protein that carries oxygen throughout the body and gives red blood its color. The percentage of blood volume composed of red blood cells is called the hematocrit. As used herein, unless otherwise limited, RBCs also includes packed red blood cells (pRBCs). Packed red blood cells are prepared from whole blood using centrifugation techniques commonly known in the art. As used herein, unless otherwise indicated, the hematocrit of pRBCs is about 50%. 
     Platelets are small cellular components of blood that facilitate the clotting process by sticking to the lining of the blood vessels, and also facilitate healing by releasing growth factors when activated. The platelets, like the red blood cells, are made by the bone marrow and survive in the circulatory system for 9 to 10 days before they are removed by the spleen. Platelets are typically prepared using a centrifuge to separate the platelets from the buffy coat sandwiched between the plasma layer and the pellet of red cells. 
     Plasma is a protein-salt solution and the liquid portion of the blood in which red and white blood cells and platelets are suspended. Plasma is 90% water and constitutes about 55 percent of the blood volume. One of the primary functions of plasma is to assist in blood clotting and immunity. Plasma is obtained by separating the liquid portion of the blood from the cells. Typically, plasma is separated from the cells by centrifugation. Centrifugation is the process used to separate the components of the whole blood into the plasma, the white blood cells, the platelets and the packed red blood cells. During centrifugation, the plasma will initially migrate to the top of a vessel during a light spin. The plasma is then removed from the vessel. The white blood cells and platelets are removed during a second centrifugation cycle to produce the packed red blood cells. 
     The present disclosure includes and provides for methods for preparing oxygen depleted blood for storage. An oxygen reduced blood or blood component suitable for storage and benefiting from the reduced damage from storage lesions, reduced toxicity, and importantly reduced morbidity, is a blood or blood component having an oxygen saturation of less than about 20%. In certain aspects, the oxygen levels in the blood or blood component are reduced to a level of less than 15%. In other aspects, the oxygen saturation of the blood is reduced to 10% or less prior to storage. In yet another aspect, the oxygen saturation of the blood is reduced to less than 5% or less than 3% prior to storage. 
     According to methods of the present disclosure, the blood or blood component is depleted of oxygen and placed into storage within 4 to 24 hours of collection. In other aspects, the methods provide for the depletion of oxygen and placement into storage within 8 hours of collection. In other aspects, the blood or blood component is depleted of oxygen and placed into storage in less than 6 hours of collection. In yet another aspect, the blood is depleted of oxygen and placed into storage in less than 4 hours of collection. 
     The present disclosure provides for, and includes, methods for preparing blood for storage under oxygen depleted conditions comprising providing blood having red blood cells having oxygen to be removed to an oxygen depletion device  10 , and incubating the blood for a period of time. In certain aspects, the blood is mixed through agitation. In other aspects, the oxygen depletion device provides for sufficient deoxygenation with little or no mixing. 
     As would be understood, blood for depletion may start with varying levels of oxygen saturation. In certain aspects, the blood is whole blood collected at about 70% saturation and between about 40% to 45% hematocrit. The methods of the present disclosure also provide for the rapid deoxygenation of LRpRBCs that typically have a hematocrit of about 50% and saturation levels of up to 90% or higher. 
     The devices and methods of the present disclosure are intended to provide oxygen depleted blood for storage within 24 hours or less. In certain aspects, the oxygen is removed using an oxygen depletion device  10  by incubation for a time period with agitation. In other aspects, the oxygen is removed using an oxygen depletion device  10  using methods in which the depletion device is not agitated or otherwise mixed during the incubation period. As would be understood by one of skill in the art, inclusion of an agitation or mixing step in the process allows for an oxygen depletion device  10  to have lower surface to volume ratios. Agitation can also reduce the permeability necessary to achieve a desired level of deoxygenation. To achieve the most rapid depletion kinetics, a combination of an oxygen depletion device  10  having high permeability and a high surface to volume ratio is combined with agitation during the depletion period. Depending on the application and the processing protocols employed, the time necessary to complete processing can vary to between 4 and 24 hours. Thus, the devices and methods of the present disclosure can be incorporated into the protocols of existing blood processing centers and comply with applicable regional regulations by adjusting the devices and methods as provided in the present disclosure. 
     In aspects according to the present disclosure, to reduce the processing time to achieve a blood saturation of less than 20%, the blood can be agitated or mixed during the depletion period. In most aspects, the blood is agitated or mixed for less than 24 hours. As mixing and agitation of the blood during processing can lead to lysis and degradation, the depletion time period with agitation should be minimized. 
     In certain aspects, the blood is incubated with agitation for less than 12 hours. In other aspects, the incubation and agitation time is less than 8 hours. Also provided for are methods for reducing oxygen to less than 20% using an oxygen depletion device  10  and incubating with agitation for less than 6 hours or less than 4 hours. In yet other aspects, the incubation time with agitation is 3.5 hours or 3.0 hours. In certain aspects, the blood can be reduced to 20% or less with a 4-hour incubation with agitation in an oxygen depletion device  10 . In yet further aspects, the method provides for incubation times of 0.5 or 1.0 hour. In other aspects, blood is incubated for 1.5 hours or 2.0 hours in an oxygen depletion device  10 . 
     It is well understood that reaction rates are temperature dependent, with higher temperatures increasing the reaction rate. The rate constant k varies exponentially with temperature where k=Ae −Ea/RT  (the Arrhenius equation). Notably, the dependence on temperature is independent of the concentration of reactants and does not depend on whether the order of the rate is constant (e.g., first order vs. second order). Typically, a 10° C. increase in temperature can result in a two fold increase in reaction rate. Accordingly, a person of skill in the art would recognize that the release of oxygen from hemoglobin as well as the other steps of the deoxygenation process is temperature dependent. Importantly, once the temperature of the blood is lowered to the standard storage temperature of between 2° C. and 6° C., the rate of deoxygenation is significantly reduced. Even further, under current approved protocols for the collection, processing and storage of blood for transfusion purpose conditions, the stored blood is not mixed which further reduces the rate at which oxygen can be removed. Accordingly, the methods and devices of the present disclosure are designed to remove most of the oxygen prior to storage and within the time periods established by the appropriate regulatory agencies. As provided herein, the depletion of oxygen is intended to begin as soon after collection from the donor as possible, and is intended to be largely completed prior to cooling the blood for storage. 
     As provided herein, the methods can be performed using recently collected blood that is about 37° C., when it is collected from the donor. In other aspects, the blood may be processed prior to depletion, including removing leukocytes, plasma, and platelets. In the alternative, the blood may be further processed after oxygen reduction. 
     The present disclosure provides for, and includes, processing blood that has cooled from body temperature to ambient temperature, typically about 25° C. Using the methods and devices disclosed here, oxygen reduced blood having less than 20% oxygen saturation can be prepared at ambient temperatures (e.g., about 25° C.). The ability to reduce the oxygen to desired and beneficial levels at ambient temperatures allows for the systems and methods to be incorporated into existing blood collection protocols and blood collection centers. 
     The present disclosure provides for, and includes, methods for preparing blood for storage under oxygen depleted conditions comprising providing blood having red blood cells having oxygen to be removed to an oxygen depletion device  10 , incubating the blood for a period of time and further comprising agitating or mixing during the incubation period. As used herein, the terms “agitating” or “mixing” are used interchangeably and include various mixing methods, including but not limited to rocking, nutating, rotating, stirring, massaging, swinging, linearly-oscillating and compressing the oxygen depletion device. 
     In a method according to the present disclosure, the incubation period with agitation can be as short as 30 minutes and up to 24 hours. In certain aspects, the method includes an incubation period of between 1 and 3 hours with agitation in an oxygen depletion device  10 . In other aspects, the incubation period is between 1 and 4 hours or 1 and 6 hours. In other aspects, the incubation period is about 2 hours or about 4 hours. 
     In an aspect according to the present disclosure, a method of reducing the oxygen from red blood cells includes placing the red blood cells in a device according to the present disclosure and placing the device on an agitator to enhance the oxygen removal from the red blood cells through a mixing action. The use of agitators in the practice of blood transfusion is well known with respect to preventing clot formation, such as in the use of rocker tables and donation scale mixers, which provide for a gentle rocking motion of about 7 degrees tilt and 1 to about 15 oscillations per minute. Similar devices, already available in oxygen depletion centers and familiar to staff, can be used to ensure proper mixing. 
     To maximize the kinetics of the oxygen depletion process, both physical and methodological approaches can be applied. As discussed above, physical approaches to reducing the resistance to diffusion of the inner blood compatible bag is achieved by selecting materials with high permeability and by reducing the thickness of the material to decrease the Barrer value. For microporous materials, apparent Barrer values can be decreased by decreasing the size of the micropores and by increasing the number of micropores. It is understood that the size of micropores are necessarily limited by the necessity to prevent the perfusion of water through the barrier which occurs in certain microporous materials at about 1 μm. Also as provided above, the surface to volume ratio is selected to reduce the diffusion distance of the dissolved oxygen as it makes its way to the permeable surface. These limitations and requirements of the materials and design to achieve effective and rapid reduction of oxygen in blood are discussed above. 
     In addition to minimizing the diffusion barriers and the diffusion distance through design and by appropriate selection of materials, the effective diffusion distance can be further reduced by appropriate mixing. As would be understood, complete and efficient mixing effectively eliminates the effect of diffusion distance on the blood reduction process as oxygen containing red blood cells enter the oxygen free environment in close proximity to the permeable membrane. Similarly, the diffusion distance would also be eliminated by spreading the blood into an impracticably thin volume. The present disclosure provides methods and devices that optimize the devices and methods to achieve high rates of depletion. 
     The present disclosure provides for, and includes, methods of mixing blood in an oxygen depletion device  10  that achieves rapid rates of deoxygenation and rate constant of between about 0.5×10 −2  min −1  and about 5.0×10 −2  min −1 . In aspects according to the present disclosure, the rate constant is at least −1.28×10 −2  min −1 . In other aspects, deoxygenation occurs at rate having a rate constant of at least −0.5×10 −2 . In another aspect, deoxygenation occurs at rate having a rate constant of at least −0.9×10 −2 . In another aspect, deoxygenation occurs at rate having a rate constant of at least −1.0×10 −2 . In another aspect, deoxygenation occurs at rate having a rate constant of at least −1.5×10 −2 . In further aspects, deoxygenation occurs at rate having a rate constant between −1.0×10 −2  min −1  and −3.0×10 −2  min −1 . In further aspects, deoxygenation occurs at rate having a rate constant between −1.0×10 −2  min −1  and −2.0×10 −2  min −1 . In further aspects, deoxygenation occurs at rate having a rate constant between −1.0×10 −2  min −1  and −4.0×10 −2  min −1 . 
     In an aspect according to the present disclosure, proper mixing is achieved in an oxygen depletion device  10  having a surface to volume ratio of at least 5.0 cm 2 /ml. Not to be limited by theory, it is hypothesized that at lower surface to volume ratios, the collapsible blood container does not have sufficient capacity to allow for the movement of blood and no mixing occurs. It would be appreciated that a bag, filled to capacity like an engorged tick, would be essentially refractory to mixing and no convection or other currents could be readily induced. In other words, an inner collapsible container  102  that is filled to capacity to the extent that the flexibility of the bag material is reduced beyond its ability to yield during agitation results in essentially no mixing. Accordingly, by selecting a surface to volume ratio of at least 4.85 cm 2 /ml mixing can occur as the blood is ‘sloshed’ around. It would be appreciated that improper mixing leads to undesirable hemolysis of the red blood cells. Accordingly, mixing also has practical limits. The present disclosure provides for devices and methods to reduce potential hemolysis while achieving significant mixing. 
     In an aspect according to the present disclosure, a method of reducing the oxygen from red blood cells includes placing the red blood cells in a device according to the present disclosure and placing the device on an agitator to enhance the oxygen removal from the red blood cells. The use of agitators in the practice of blood transfusion is well known with respect to preventing clot formation, such as in the use of rocker tables and donation scale mixers when used with whole blood and suspensions of red blood cells, and also for platelet storage, wherein the platelets require oxygen for survival and agitation to prevent clumping and activation of the platelets. 
     With respect to currently available devices for agitating red blood cells, whether whole blood or other red blood cell suspensions, a platform is typically rotated a few degrees about a central axis to provide for a gentle rocking motion and there are many available choices commercially available. For example, Bellco Glass model #7740-10000 (Bellco Glass, Inc., Vineland, N.J.) provides for 7 degrees tilt and 1 to about 12 oscillations per minute. The Medicus Health model 5277M5 nutating mixer (Medicus Health, Kentwood, Mich.) provides for a 20 degree angle of inclination at 24 rpm for the suspension of red blood cell samples, while another style device used at the time of donation to prevent clotting of whole blood is the Genesis blood collection mixer model CM735A (GenesisBPS, Ramsey, N.J.), which provides for about 20 degrees of tilt and performs 3 cycles in about 3 seconds, then rests for about 2 seconds to weigh the sample and repeats until the desired weight is achieved. The Benchmark Scientific model B3D2300 (Benchmark Scientific, Inc., Edison, N.J.) provides for a variable 0-30 degree tilt angle and 2-30 oscillations per minute. 
     The present disclosure further includes and provides for other available means of agitation of blood samples including orbital shakers, such as the model LOS-101 from Labocon (Labocon Systems, Ltd, Hampshire, U.K.), having a displacement of 20 mm and an oscillation rate of 20-240 rpm, or the model EW-51820-40 from Cole-Parmer (Cole-Parmer, Inc., Vernon Hills, Ill.) having a displacement of 20 mm and an oscillation rate of 50-250 rpm. 
     Devices to agitate platelets are also well known in the art and include various models such as the PF96 h from Helmer Scientific (Helmer Scientific, Noblesville, Ind.) which provides for a linear oscillation of about 70 cycles per minute with a displacement of about 38 mm (1.5 inches), and the model PAI 200 from Terumo Penpol (Terumo Penpol Ltd., Thiruvananthapuram, India) with an oscillation of about 60 cycles per minute and a displacement of about 36 mm (1.4 inches). 
     While the devices of the present disclosure provide for enhanced deoxygenation of red blood cells, the use of modified motions provides for even further oxygen removal from the red blood cells. It is well known that platelets can be activated by mechanical agitation, such as shear force, and are therefore subject to limitations on how much physical agitation can be tolerated before such activation occurs. Hemolysis of red blood cells is estimated to occur at shear stress levels above approximately 6000 dyne/cm 2  (Grigioni et al.,  J. Biomech.,  32:1107-1112 (1999); Sutera et al.,  Biophys.  1, 15:1-10 (1975)) which is an order of magnitude higher than that required for platelet activation (Ramstack et al.,  J. Biomech.,  12:113-125 (1979)). In certain aspects, currently available platelet agitators operating at about 36 mm displacement and about 65 cycles per minute (cpm) provide for deoxygenation of red blood cells as disclosed herein. In other aspects, improved rates and extent of deoxygenation without hemolysis are achieved using linear oscillating motion with a displacement of between 30 mm and about 125 mm. In another aspect, the agitation is a linear oscillation from about 50 mm to about 90 mm. 
     The present disclosure also provides for, and includes, adjusting the frequency of oscillation to ensure efficient mixing. In addition to platelet shakers having a displacement of about 36 mm and a frequency of about 65 cpm, in certain aspects, the frequency is from about 60 to about 150 cycles per minute (cpm). In certain aspects, the frequency of agitation is between about 80 and about 120 cpm. 
     In certain aspects according to the present disclosure, when using an agitator or mixer, various configurations of the chambers in a collection device  10  with more than one chamber are provided. With an agitator that moves in a horizontal motion, in one aspect, two to eight horizontal (flat on surface) chambers are arranged, side by side, end to end, one on top of the other, one on top of the other with one or more partially covering the chamber(s) beneath. In other aspects, with an agitator that moves in a vertical motion, two to eight vertical (perpendicular to surface) chambers are arranged, side by side, end to end, one on top of the other, one on top of the other with one or more partially covering the chamber(s) beneath. In another aspect, with an agitator that moves up and down traversing an angle&gt;0 and &lt;90 degrees to horizontal two to eight upright (&gt;0 degrees&lt;90 degrees to horizontal) chambers are arranged, side by side, end to end, one on top of the other, one on top of the other with one or more partially covering the chamber(s) beneath. 
     A further advantage of the agitation and mixing of the oxygen depletion device  10  is that the movement of the blood or blood component caused by the agitator also moves the sorbent sachet located on the top or bottom of the oxygen depletion device  10 . Because the sorbent sachet is moving up and down as it rests on top, the active ingredient that absorbs the oxygen in the headspace is constantly settling. This constant movement of the active ingredient moves oxidized iron particles out of the way of non-oxidized iron particles, speeding up the oxygen absorption potential of the sorbent. 
     The present disclosure provides for, and includes, mixing the oxygen depletion device  10  by compression of the collapsible blood container  102 . Compressing of the collapsible container  102  is achieved by applying pressure on one of the collapsible container&#39;s larger surfaces at 30 cm/sec during 1-3 seconds creating a hydrostatic pressure of 100-300 mmHg within the collapsible container, then applying a pressure to the opposite surface of the collapsible container at 10-30 cm/sec during 1-3 seconds creating a hydrostatic pressure of 100 to 300 mmHg within the collapsible container. This operation is to be performed for 2 to 4 hours. 
     The present disclosure provides for, and includes, mixing the oxygen depletion device  10  by massage of the collapsible blood container  102 . Massaging of the collapsible container  102  is achieved by displacing a roller type device along one surface of the collapsible container completing the full translation in 1-3 seconds and making the collapsible container collapse and agitate its contents. This operation is to be performed for 1-2 hours. In another aspect, a roller travels along another surface of the collapsible container, completing the full translation in 1-3 seconds and making the collapsible container collapse and agitate its content. This operation is to be performed for 1-2 hours. 
     The present disclosure provides for, and includes, a blood storage device  20 , for storing oxygen depleted blood and maintaining the blood in a deoxygenated state during the storage period. Certain anaerobic blood storage devices (ASB) are known in the art, including for example U.S. Pat. No. 6,162,396 to Bitensky et al. The anaerobic blood storage devices of the prior art did not include ports and inlets designed to be substantially impermeable to oxygen. Accordingly, the prior art anaerobic storage devices had poor shelf lives prior to use and were susceptible to significant ingress of oxygen. As provided in the present disclosure, an improved blood storage device  20  comprising features directed to maintaining the integrity of the device while allowing for the sampling of the blood that occurs during storage and blood banking. The improved ASB also provides for improved diffusion of oxygen from the blood, providing for additional depletion during the storage period. 
     The blood storage device  20  comprises an outer receptacle  201  that is substantially impermeable to oxygen, a collapsible blood container  202  comprising a locating feature  203  adapted to align the collapsible blood container  202  within the geometry of the outer receptacle  201 ; at least one inlet/outlet  30  comprising connecting to the collapsible blood container  202  and a bond  302  to the outer receptacle  201 , wherein the bond  302  to the outer receptacle  201  is substantially impermeable to oxygen and an oxygen sorbent  207  situated within the outer receptacle  201 . 
     As used herein, an outer receptacle  201  is at least equivalent to an outer receptacle  101 . Also as used herein, an inner collapsible blood container  202  includes blood containers as provided above for an inner collapsible blood container  102  but also provides for collapsible blood containers  202  comprising materials that are less permeable to oxygen, such as PVC. Also as provided herein, oxygen sorbent  207  is at least equivalent to sorbent  103  and may be provided in sachets as discussed above. 
     Also included and provided for in the present disclosure are blood collecting kits. In an aspect according to the present disclosure, an oxygen depletion device for depleting oxygen from blood is included in a blood collection kit that reduces or eliminates the introduction of oxygen during the blood collection process. Blood collection kits in the art do not include any features or elements that prevent the introduction of oxygen during the collection process. Accordingly, kits in the art having multiple containers provide from about 3 cc&#39;s of residual oxygen per container, plus additional ingress through materials and fittings, and thereby increase the saturation of oxygen (sO 2 ) from the venous oxygen saturation (SvO2) of about 40 to 60% to up to full saturation. In an aspect according to the present disclosure, the entire blood collection kit is contained in an oxygen free or oxygen reduced environment. In an aspect, the blood collection kit is contained within a kit enclosure bag that is substantially oxygen impermeable and includes within the enclosure bag an amount of oxygen sorbent absorb oxygen. Amounts of sorbent for a blood collection kit according to the present disclosure are separate from, and in addition to amounts of sorbent that may be included in a blood collection bag or anaerobic storage bag. 
     In certain aspects according to the present disclosure, the amount of oxygen sorbent included in a blood collection kit is sufficient to remove oxygen from a blood collection kit introduced during manufacture. In an aspect, the blood collection kit includes oxygen sorbent sufficient to absorb 10 cc&#39;s of oxygen. In another aspect, the blood collection kit includes oxygen sorbent sufficient to absorb 60 cc&#39;s of oxygen. In another aspect, the blood collection kit includes oxygen sorbent sufficient to absorb 100 cc&#39;s of oxygen. In another aspect, the blood collection kit includes oxygen sorbent sufficient to absorb 200 cc&#39;s of oxygen. In another aspect, the blood collection kit includes oxygen sorbent sufficient to absorb 500 cc&#39;s of oxygen. In another aspect, the blood collection kit includes oxygen sorbent sufficient to absorb from 10 to 500 cc&#39;s of oxygen. In another aspect, the blood collection kit includes oxygen sorbent sufficient to absorb up to 24,000 cc to allow for the management of shelf life of the device. In certain aspects according to the present disclosure, the oxygen sorbent is disposed in one or more sachets. 
     In an aspect, the amount of oxygen sorbent is sufficient to maintain an oxygen depleted environment for the blood collection kit during the storage. In certain aspects, oxygen is flushed from the blood collection kit during manufacture. Accordingly, the amount of oxygen sorbent may be reduced to account for leakages and residual permeability of the substantially impermeable kit enclosure bag. 
     Also included and provided for in the present disclosure are additive solution bags that are substantially impermeable to oxygen. In an aspect according to the present disclosure, substantially oxygen impermeable additive solution bags avoid the reintroduction of oxygen to the oxygen reduced blood after oxygen reduction in the oxygen reduction blood collection bag. 
     In aspects of the present disclosure, the method may further include adding an additive solution to the packed RBCs to form a suspension. In certain aspects, the additive solution may be selected from the group consisting of AS-1, AS-3 (Nutricel®), AS-5, SAGM, PAGG-SM, PAGG-GM, MAP, SOLX, ESOL, EAS61, OFAS1, and OFAS3, alone or in combination. Additive AS-1 is disclosed in Heaton et al., “Use of Adsol preservation solution for prolonged storage of low viscosity AS-1 red blood cells,”  Br J Haematol.,  57(3):467-78 (1984). In a further aspect, the additive solution may have a pH of from 5.0 to 9.0. In another aspect, the additive may include an antioxidant. In some aspects according the present disclosure, the antioxidant may be quercetin, alpha-tocopheral, ascorbic acid, or enzyme inhibitors for oxidases. 
     EXAMPLES 
     Example 1: Fabrication of Outer Receptacle  101   
     A barrier bag is fabricated by heat sealing along one edge by placing a pair of RollPrint Clearfoil® Z film #37-1275 (Rollprint Packaging Products, Inc., Addison, Ill.) sheets about 23×30.5 cm (9×12 inches) into a heat sealer along the shorter 23 cm length. A piece of multilayer tubing having a polyethylene outer layer, a PVC inner layer, and an intermediary bonding layer of EVA (Pexco, Inc., Athol, Mass. or Extrusion Alternatives, Inc., Portsmouth, N.H.) 0.4 cm I.D. by 0.55 cm O.D. by about 2.6 cm long is placed onto a solid brass mandrel about 0.4 cm diameter by about 2.5 cm length and then placed between the films and located in the transverse groove of the heat sealing dies heated to about 130° C. The press is activated and set to about 4 seconds duration at 21×10 4  Pascal (Pa) to create a continuous welded seal along the length of the dies, with the short piece of multilayer tubing sealed in place. The short multilayer tubing provides for an oxygen impermeable seal around the outer diameter of the tubing while also providing fluid connectivity through the seal. A piece of PVC tubing 0.3 cm I.D.×0.41 cm O.D. by about 30.5 cm length (Pexco, Inc., Athol, Mass., or Extrusion Alternatives, Inc., Portsmouth, N.H.) is solvent bonded using cyclohexanone to the multilayer tubing from the outside of the bag. 
     Sealing of the two long edges of the barrier film is performed with an impulse heat sealer (McMaster Carr #2054T35, McMaster Carr, Inc., Robbinsville, N.J.), leaving the last remaining short edge of the barrier bag unsealed to place a blood container  102  inside. 
     Example 2: Preparation of Silicone Sheets 
     Liquid Silicone Rubber (LSR) 
     Silicone sheets having a thickness of about 25 μm are fabricated by mixing equal parts of a two-part silicone elastomer dispersion in a suitable solvent, such as xylene, for example NuSil MED10-6640. MED 10-6640 is supplied as a 2 part resin system. As the first step, Part A and Part B are mixed in equal measure to create the dispersion. Next, the air was removed under vacuum. The vacuum time was selected to ensure that no bubbles were left in the dispersion. Next the dispersion is spread out and passed under a precision knife edge on a custom built knife coating tray. The sheet is partially cured by heating before placing a sheet of polyester mesh fabric (Surgical Mesh, Inc., Brookfield, Conn. #PETKM3002) onto the partially cured silicone sheet. The polyester mesh fabric is pressed into the partially cured sheet by applying a load onto the laminate. The laminate is cured using a ramp cure using the following sequence of time and temperature combination: 30 minutes at ambient temperature and humidity, 45 minutes at 75° C. (167° F.), and 135 minutes at 150° C. (302° F.) to yield a silicone membrane  113  about 25 μm thick, and having an integrated spacer  110 , whose thickness is not inclusive of the resulting silicone membrane. The polyester mesh fabric is adhered to the cured silicone membrane  113 , but was not totally encapsulated by the silicone membrane  113 . The one surface of the membrane  113  has a matte finish suitable for contact with blood or blood products. 
     Additional integrated silicone membranes having thicknesses of about 13 μm and about 50 μm are fabricated using the silicone dispersion method. 
     Example 3: Fabrication of an Inner Collapsible Blood Container  102   
     A silicone blood bag is fabricated from a pair of silicone sheets by bonding the edges together with Smooth On Sil-Poxy RTV adhesive (Smooth-On, Inc. Easton, Pa.) and placing the bonded sheets between a pair of flat aluminum plates to yield a silicone blood bag. A silicone inlet tube (McMaster Carr #5236K83, McMaster Carr, Inc., Robbinsville, N.J.) is bonded within the seam to provide for fluid passage and nested within a groove in the aluminum plates before clamping the plates together with large binder clamps and allowing the adhesive to cure overnight. The silicone blood bag is removed from the aluminum plates the next day and leak tested by insufflating with compressed air and submerging in water to observe for bubbles before use. The silicone blood bag is then placed in an outer barrier bag fabricated as described in Example 1. 
     The silicone blood bag is placed inside the barrier bag as disclosed in Example 1 and the silicone inlet tube of the silicone blood bag is connected to the multilayer tube using a plastic barb fitting (McMaster Carr #5116K18, McMaster Carr, Inc., Robbinsville, N.J.), and an oxygen sensor tab (Mocon #050-979, Mocon, Inc., Minneapolis, Minn.) is affixed to the inside of the barrier bag. A pair of plastic mesh spacers (McMaster Carr #9314T29, N.J. McMaster Carr, Inc., Robbinsville, N.J.) are cut to about 12.7×17.8 cm (5×7 inches) and one or more sachets of oxygen sorbent (Mitsubishi Gas Chemical America, New York, N.Y.) are affixed near the center of each piece of plastic mesh just seconds prior to placing the plastic mesh spacers between the blood bag and barrier bag and sealing the final edge of the barrier bag with the impulse sealer. The resulting oxygen depletion device  10  is used in subsequent testing. 
     Example 4: Blood Preparation 
     Whole blood and blood products including leukoreduced whole blood and leukoreduced packed red blood cells are prepared using techniques known in the art. Samples are analyzed as indicated using a Radiometer ABL-90 hemoanalyzer (Radiometer America, Brea, Calif.) according to manufacturer instructions including pH, blood gas, electrolyte, metabolite, oximetry, and baseline sO 2 , and pO2 levels. Free hemoglobin is measured using the Hemocue® Plasma Low Hb Photometer according to manufacturer instructions. 
     As appropriate, blood sO 2  levels are increased to levels typical of collected whole blood (65 to 90%) by passing the blood or blood component through a Sorin D100 oxygenator (Arvada, Colo.) with oxygen as the exchange gas. All experiments begin with ≥50% sO 2  prior to transferring the blood to an oxygen depletion device for testing. 
     Example 5: Test of Deoxygenation 
     An oxygen depletion device of Example 2 is provided with blood and tested as follows. Whole blood (124 grams) is obtained and saturated with oxygen by injecting several cc of pure oxygen gas and placed in the silicone blood bag of Example 2 by sterile transfer using a Terumo Sterile Connection Device (SCD) and weighing the bag during transfer. The outer receptacle  101  headspace oxygen level is measured using a Mocon OpTech Platinum oxygen analyzer and determined to be 1.60 torr at the start of the experiment. An initial sample of blood is taken and measured on a Radiometer ABL-90 hemoanalyzer (Radiometer America, Brea, Calif.) and the saturated oxygen content (sO2) found to be 98.7%. The barrier bag with blood is placed on a work bench at room temperature (21.0° C.) and allowed to stand one hour without agitation. After one hour, the sO2 is determined to be 93.5% sO2 and the barrier bag headspace oxygen is determined to be 0.70 torr oxygen. The barrier bag with blood is incubated at room temperature (21.0° C.) for about 14 hours without agitation. After 14 hours incubation, the sO 2  is determined to be 66.7%, and a final determination of sO 2  is 51.2% after an additional 7 hours incubation at 21° C. without agitation. The rate of deoxygenation follows first order kinetics and the rate constant is calculated to be on the order of about min −1 . 
     Example 6: Serpentine Urethane Flow Oxygen Depletion Devices 
     A collapsible blood bag is fabricated from breathable polyurethane film (American Polyfilm, Branford, Conn.) having a reported moisture vapor transmission rate of 1800 gr/m 2 /24 hrs., wherein a serpentine tortuous flow path is fabricated using a custom heat sealing die to weld a pair of the films together to create the geometry. The collapsible bag with tortuous path comprised a series of 12 channels of about 5 mm width and 220 mm in length, providing for an overall flow path of about 2640 mm. The collapsible bag is sealed within an outer barrier according to Example 1. The resulting depletion device further includes two multilayer tubes sealed within one end, as previously described in this disclosure, such that the inlet and outlet of the tortuous path are in fluid connectivity with the pieces of multilayer tube. 
     Two pieces of plastic spacer mesh (McMaster Carr #9314T29, McMaster Carr, Inc., Robbinsville, N.J.) are cut to about 125×180 mm (5×7 inches) and placed on both sides of the collapsible blood container within the outer barrier receptacle. A sachet of oxygen sorbent (SS-200, Mitsubishi Gas Chemical America, NY, N.Y.) is placed between each of the plastic mesh spacers and the outer barrier receptacle (2 sachets total) and an oxygen sensor tab (Mocon #050-979, Mocon, Inc. Minneapolis, Minn.) before sealing the final edge of the outer barrier receptacle. A length of standard IV tubing (Qosina T4306, Qosina, Corp., Edgewood, N.Y.) 914 mm (36 inches) is solvent bonded using cyclohexanone to each of the multilayer tubes. A ratchet clamp (Qosina #140072, Qosina, Corp., Edgewood, N.Y.) is placed onto the outlet tubing to control flow. 
     A standard 500-mL blood bag (model KS-500, KS Mfg., Avon, Mass.) is connected to the length of outlet tubing using a Terumo sterile tubing welder (model TSCD-II, Terumo BCT, Inc., Lakewood, Colo.). A second standard 500-mL blood bag (model KS-500, KS Mfg., Avon, Mass.) is filled with 325 grams of blood at 20.8° C. and a sample measured on a Radiometer ABL-90 hemoanalyzer (Radiometer America, Brea, Calif.) and found to have 83.0% sO 2  and 70.1 mm Hg pO 2 . A ratchet clamp (Qosina #140072, Qosina, Corp., Edgewood, N.Y.) is placed onto the inlet tubing to control flow and the filled blood bag is then connected to the inlet tube using a Terumo sterile tubing welder (model TSCD-II, Terumo BCT, Inc., Lakewood, Colo.). The ratchet clamps are closed to prevent flow and the filled blood bag is hung from an IV pole such that the inlet tubing was fully extended and the collapsible blood bag is on the laboratory bench. The outlet bag is tared on a balance before it is placed on the floor with the outlet tubing fully extended. The headspace oxygen level in the outer receptacle is measured using a Mocon Op-Tech platinum oxygen analyzer and found to be 0.05 torr oxygen at the start. The clamps are opened and a stopwatch timer started to measure the duration of flow, and after 3 minutes 25 seconds the inlet blood bag is emptied and the ratchet clamps are closed. A sample of blood is taken and measured and found to have 84.1% sO 2  and 71.6 mmHg pO 2 , the increase presumably from residual oxygen in the empty circuit. The headspace measured 0.00 torr oxygen and the outlet blood bag contained 277 grams of blood. 
     The empty inlet blood bag is removed from the IV pole and placed on the floor, while the outlet blood bag with 277 grams of blood is hung from the IV pole to repeat the flow. The IV pole is lowered to 457 mm (18 inches) to reduce the flow rate and the clamps opened to repeat the cycle. The process is repeated 5 times, and then the collapsible blood bag is filled with blood and allowed to remain stationary on the laboratory bench for 80 minutes and a terminal blood sample is taken for measurements on the hemoanalyzer. The table below summarizes the results, which show a slight gradual increase in the oxygen level of the blood during flow, with a slight decrease after standing. The results indicate that the system does not provide for an appreciable deoxygenation of the blood over the course of the study, but rather absorbs oxygen from the permeable standard PVC blood bags. From this, the importance of taking additional measures to prevent the ingress of oxygen at inlets, outlets, ports, and tubing is demonstrated. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Deoxygenation using urethane bags 
               
            
           
           
               
               
               
               
               
            
               
                 Flow 
                 Time 
                   
                   
                 Headspace 
               
               
                 Pass # 
                 (min:sec) 
                 sO 2 % 
                 pO 2  mmHg 
                 O 2  torr 
               
               
                   
               
               
                 Start 
                 n/a 
                 83.0 
                 70.1 
                 0.05 
               
               
                  1* 
                 3:25 
                 84.1 
                 71.6 
                 0.00 
               
               
                 2 
                 7:45 
                 84.8 
                 72.6 
                 0.09 
               
               
                 3 
                 6:38 
                 85.2 
                 72.9 
                 0.09 
               
               
                 4 
                 7:16 
                 85.6 
                 73.4 
                 0.13 
               
               
                 5 
                 6:31 
                 85.4 
                 72.9 
                 0.15 
               
               
                 Stagnant 
                 80:00  
                 83.6 
                 64.3 
                 0.15 
               
               
                   
               
               
                 *914 mm head height; all other flow passes at 457 mm height. 
               
            
           
         
       
     
     Example 7: Test of Inner Collapsible Blood Container  102  Configurations 
     A series of inner collapsible blood containers  102  are prepared according to Table 4 below and sealed in an outer receptacle  101  as provided in Example 1. Leukoreduced packed red blood cells (LRpRBC) are introduced into the container  102 . The resulting oxygen depletion devices  10  further included a Mocon Optech-O2 sensor. Assembled blood containers according to Table 4 are placed on a Helmer Labs Platelet Shaker, Model PF96 and blood and headspace samples are obtained and analyzed at time points between 0 and 300 minutes. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Inner collapsible blood container 102 test configurations 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Thickness 
                   
                 # 
               
               
                   
                 LRpRBC 
                   
                   
                   
                 or Pore 
                   
                 Sorbent 
               
               
                 Sample 
                 Hct 
                 sO 2i   
                 Material 
                 Source 
                 size 
                 Compartments 
                 Sachets* 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 1A 
                 &gt;45%  
                 &gt;80% 
                 Silicone 
                 Wacker 
                 30 
                 μm 
                 Double 
                 10 
               
               
                   
                   
                   
                   
                 Silpuran 
               
               
                 2A 
                 &gt;45%  
                 &gt;80% 
                 Silicone 
                 McMaster- 
                 127 
                 μm 
                 Single 
                 2 
               
               
                   
                   
                   
                   
                 Carr 
               
               
                 3A 
                 &gt;45%  
                 &gt;80% 
                 Silicone 
                 Polymer 
                 76 
                 μm 
                 Triple 
                 15 
               
               
                   
                   
                   
                   
                 Science 
               
               
                 4A 
                 &gt;45%  
                 &gt;80% 
                 Silicone 
                 Polymer 
                 76 
                 μm 
                 Triple 
                 15 
               
               
                   
                   
                   
                   
                 Science 
               
               
                 5A 
                 &gt;45%  
                 &gt;80% 
                 PVDF 
                 Millipore 
                 0.22 
                 μm 
                 Singe 
                 5 
               
               
                   
                   
                   
                 GVSP 
               
               
                 1B 
                 52% 
                 &gt;50% 
                 PVDF 
                 Millipore 
                 0.22 
                 μm 
                 Single 
                 5 
               
               
                   
                   
                   
                 VVSP 
               
               
                 2B 
                 52% 
                 &gt;50% 
                 PVDF 
                 Millipore 
                 0.1 
                 μm 
                 Single 
                 5 
               
               
                   
                   
                   
                 VVSP 
               
               
                 3B 
                 52% 
                 &gt;50% 
                 PVDF 
                 Millipore 
                 1.0 
                 μm 
                 Single 
                 5 
               
               
                   
                   
                   
                 VVSP 
               
               
                 4B 
                 52% 
                 &gt;50% 
                 Silicone 
                 Wacker 
                 50 
                 μm 
                 Single 
                 5 
               
               
                 5B 
                 52% 
                 &gt;50% 
                 Silicone 
                 Wacker 
                 20 
                 μm 
                 Single 
                 5 
               
               
                 6B 
                 52% 
                 &gt;50% 
                 Silicone 
                 Polymer 
                 76 
                 μm 
                 Single 
               
               
                   
                   
                   
                   
                 Science 
               
               
                   
               
               
                 All oxygen depletion devices include an outer receptacle 101 according to Example 1. 
               
               
                 All examples incorporate a spacer 110 to maintain headspace 
               
            
           
         
       
     
     As shown in  FIG.  5   , the depletion of oxygen follows first order kinetics. The rate constants are provided in Table 5. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Rate constants 
               
            
           
           
               
               
               
            
               
                   
                   
                 Rate Constant 
               
               
                   
                 Sample 
                 (min −1 ) 
               
               
                   
                   
               
               
                   
                 Simulation 
                 −1.20 × 10 −2   
               
               
                   
                 1A 
                 −1.00 × 10 −2   
               
               
                   
                 2A 
                 −0.41 × 10 −2   
               
               
                   
                 3A 
                 −0.62 × 10 −2   
               
               
                   
                 4A 
                 −0.82 × 10 −2   
               
               
                   
                 5A 
                 −0.93 × 10 −2   
               
               
                   
                 1B 
                 −1.12 × 10 −2   
               
               
                   
                 2B 
                 n/a 
               
               
                   
                 3B 
                 −1.40 × 10 −2   
               
               
                   
                 4B 
                 −1.03 × 10 −2   
               
               
                   
                 5B 
                 −1.34 × 10 −2   
               
               
                   
                 6B 
                 −0.95 × 10 −2   
               
               
                   
                   
               
            
           
         
       
     
     Example 8: 30.5×30.5 cm (12×12 Inch) Thick Silicone Bag 
     A collapsible blood container  102  is fabricated from a pair of silicone sheets 152 μm thick and 228 μm thick, respectively (McMaster Carr #87315K71, McMaster Carr, Inc., Robbinsville, N.J.), bonded together around the periphery Sil-Poxy silicone adhesive (Smooth-On, Inc., Easton, Pa.) and bonding silicone tubing (McMaster Carr #9628T42, McMaster Carr, Inc., Robbinsville, N.J.) for fluid communication as an inlet tube. The bonded sheets are cured two days between clamped aluminum plates. 
     The collapsible blood bag inlet tube is connected with a multilayer tube of an outer receptacle barrier bag  101  according to Example 1 with a plastic barb fitting (McMaster Carr #5116K18, McMaster Carr, Inc., Robbinsville, N.J.). The resulting outer receptacle bag  101  is leak tested by insufflation submersion as described in Example 1. 
     The device  10  is assembled with two 330×330 mm mesh spacers (McMaster Carr #9314T29, McMaster Carr, Inc., Robbinsville, N.J.) and four sachets of oxygen sorbent are affixed to each mesh spacer with tape (SS-200, Mitsubishi Gas Chemical America, NY, N.Y.), the collapsible blood container, and an oxygen sensor tab (Mocon #050-979, Mocon, Inc. Minneapolis, Minn.) inserted and heat sealed in the barrier bag  101 . The inlet tube comprised standard IV tubing (200 mm Qosina T4306, Qosina, Corp., Edgewood, N.Y.) is solvent bonded with cyclohexanone to the multilayer tube of barrier bag  101 . A ratchet clamp (Qosina #140072, Qosina, Corp., Edgewood, N.Y.) provides for control flow. 
     A pair of matched units of blood are prepared for the study by adjusting the hematocrit to 50% after centrifugation and recombining the red cells and desired amount of plasma to achieve the target hematocrit. The initial blood sO 2  is measured on a Radiometer ABL-90 hemoanalyzer (Radiometer America, Brea, Calif.) and found to have 39.0% sO 2 ; three syringes containing 30-cc of 100% oxygen were added to the blood to obtain a sO 2  level of 85.6% before starting the study. A portion of the blood is transferred to a tared standard 500-mL blood bag (model KS-500, KS Mfg., Avon, Mass.) and filled with 532 grams of blood to represent a typical 500-mL unit of donated blood having a high hematocrit and saturated oxygen level. The standard 500-mL blood bag (model KS-500, KS Mfg., Avon, Mass.) is connected to the length of inlet tubing using a Terumo sterile tubing welder (model TSCD-II, Terumo BCT, Inc., Lakewood, Colo.) and the contents transferred into the collapsible blood bag under test. 
     The collapsible blood bag is placed on a Helmer model PF96 platelet agitator (Helmer Scientific, Nobelsville, Ind.) and a sample of blood is taken and measured on a Radiometer ABL-90 hemoanalyzer (Radiometer America, Brea, Calif.) and the headspace oxygen level measured on a Mocon Op-Tech platinum oxygen analyzer (Mocon, Inc., Minneapolis, Minn.). At the beginning, the blood is found to have 84.5% sO 2  and the headspace oxygen partial pressure is 2.48 torr. Samples are agitated on the platelet agitator and samples taken and measured every 30 minutes for 150 minutes duration. The results are summarized in Table 6 below. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Deoxygenation using a 30.5 × 30.5 cm silicon bag 
               
            
           
           
               
               
               
               
               
            
               
                 Time 
                   
                   
                   
                 Headspace O 2   
               
               
                 (min) 
                 sO 2 % 
                 pO 2  mmHg 
                 pCO 2  mmHg 
                 torr 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                 84.5 
                 63.9 
                 88.9 
                 2.48 
               
               
                 30 
                 70.3 
                 40.0 
                 50.7 
                 4.63 
               
               
                 60 
                 64.9 
                 34.9 
                 43.6 
                 5.24 
               
               
                   
               
            
           
         
       
     
     The calculated deoxygenation rate constant is −0.34×10 −2  min −1 . 
     Example 9: Effect of Mixing on Oxygen Depletion 
     Four oxygen reduction bags (ORB), having a silicone inner collapsible blood container  102 , are prepared according to Example 3. The inner collapsible blood container  102 , is filled with leukocyte reduced packed red blood cells (LRPRBC), prepared according to Example 4, to obtain a surface area to volume (SAV) ratio of approximately 6 cm 2 /ml. Three LRPRBC filled ORBs are placed flat on a Helmer PF-96 Platelet Agitator (Noblesville, Ind.) or a PF-8 agitator, with the standard cycles per minute (72 cpm) or modified to a reduced-standard cpm (42 cpm) linear oscillation. A third set of filled ORB&#39;s are placed on a Benchmark 3D 5RVH6 agitator (Sayretville, N.J.). Samples are collected and analyzed at 0, 60, 120, and 180 mins for various ABL-90 outputs outlined in Example 4. 
     As shown in  FIG.  12   , 3D mixing results in the highest rate of oxygen depletion and the lowest percent sO 2  at T180 compared to the linear oscillation method of mixing. Further, a higher rate of oxygen depletion is obtained with the standard cpm linear oscillation (SLO) compared to the reduced-standard cpm linear oscillation (R-SLO). 
     Example 10: Effect of Surface to Volume Ratios on Oxygen Depletion 
     In another example, six oxygen reduction bags (ORB), having an inner collapsible blood container  102 , are prepared with Bentec silicone. LRPRBC is collected and prepared according to Example 4. The silicone inner collapsible blood containers  102 , are filled with 176, 220, 250, 270, 300, and 350 mL of LRPRBC, to provide the surface to area ratios between 3.41-6.8 cm 2 /ml as shown in Table 7. The percent sO2 is measured in the ORBs containing the various LRPRBC volumes at 0, 30, 60, 120, and 180 mins as described in Example 4. 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Surface Area to Volume Ratio 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Blood Volume (ml) 
                 176 
                 220 
                 250 
                 270 
                 300 
                 350 
               
               
                   
               
               
                 SAV Ratio (cm 2 /ml) 
                 6.8 
                 5.45 
                 4.8 
                 4.4 
                 4 
                 3.41 
               
               
                   
               
            
           
         
       
     
     As shown in  FIG.  13   , the surface area kinetic rates decrease once the SAV ratio is below 5.45 cm 2 /ml. 
     In another example, five oxygen reduction bags (ORB), having an inner collapsible blood container  102 , are prepared with PVDF instead of silicone. LRPRBC is collected and prepared according to Example 4. The PVDF inner collapsible blood containers  102  are filled with 95, 110, 220, 300, or 360 ml blood volume, to provide the surface area to volume ratios shown in Table 8. The percent sO2 is measured in the ORBs containing the various LRPRBC volumes at 0, 30, 60, 120, and 180 mins as described in Example 4. 
     As shown in  FIG.  14   , the lowest percent sO2 after 180 mins is achieved when the SAV ratio is above 5. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Surface Area to Volume Ratio 
               
            
           
           
               
               
               
               
               
               
            
               
                 Blood Volume (ml) 
                 95 
                 110 
                 220 
                 300 
                 360 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 SAV Ratio (cm 2 /ml) 
                 6.8 
                 5.9 
                 2.9 
                 2.2 
                 1.8 
               
               
                 Kinetic Rate (×100) 
                 −1.39 
                 −1.9 
                 −0.82 
                 −0.06 
                 −0.32 
               
               
                   
               
            
           
         
       
     
     In another example, four oxygen reduction bags (ORB), having single or double sided membranes (PSU or PVDF) are prepared. LRPRBC is collected and prepared according to Example 4. The inner collapsible blood containers  102  are filled with 112-118 ml LRPRBC, to provide a 50% reduction in surface area volume in the double sided membrane. The percent sO2 is measured in the ORBs containing the single or double membrane as described in Example 4. As shown in  FIG.  15   , a 50% reduction in surface area results in a 50-60% reduction in the overall kinetic rate. 
     Example 11: Preparation of Collapsible Blood Containers from Microporous Polysulfone or PVDF 
     Heat sealed polysulfone and PVDF oxygen permeable collapsible blood containers  102  are prepared. The prepared seal results in the breakdown of the microporous structure of the films to produce a crystalline area that is sensitive to the flexural stresses associated with fluid movement in the resulting containers  102 . The containers  102  are subject to leakage and breakage and are not suitable for ORB&#39;s intended for use outside of an experimental setting. 
     To overcome the inability to heat seal polysulfone or PVDF membranes in a manner suitable for use in transfusion medicine, a heat laminable “tie” layer  105  is included in the construction of the containers  102  prepared from microporous membranes  113 . As shown in  FIG.  9 B , the seal area is reinforced by pre-laminating low density polyethylene (LDPE) strips to the inside surfaces of the upper and lower membranes to align with the bag seal area. The pre-lamination results in pre-laminate seals  107  as shown in  FIG.  9 B . The two pre-laminated membranes  113  ( 114 ) are then heat sealed to form the seal  108  as shown in  FIG.  9 B . Also as shown in  FIG.  9 B , the tie layers extend beyond the width of the seal by an amount. 
     LDPE melts at about 105° C., well below the melting temperatures of polysulfone (187° C.) or PVDF (177° C.). The bag is completed by aligning the seal areas and heat sealing the upper and lower membranes together to form a bag. Without being limited to a specific mechanism, it is believed that the LDPE flows into the membrane pores, acting as a reinforcing strain relief inboard of the seal and a low temperature “tie” layer for the seal. 
     In addition to strengthening the seal between the microporous membranes  113 , the tie layer also serves as a geometric feature  121 . Thus the overall internal geometry can be readily adjusted by selecting the shape of the tie layers  105 . Examples of exemplary geometries are shown in  FIG.  10   . As shown, the geometric feature  121  provides a rounded internal geometry thereby avoiding reduced mixing associated with corners. As shown in  FIG.  10   , the resulting container  102  can be oval or round and may further include a mixing feature  119  which provides for a circular flow of blood product and enhances mixing. 
     An inner collapsible blood bag is fabricated from a pair of Millipore PVDF membranes having 0.22 μm pore size, 177×177 mm square, by first heat bonding a low density polyethylene (LDPE) tie layer frame to each membrane. The LDPE tie layer frame has a thickness of about 0.02-0.10 mm and is about 177×177 mm square on the outside dimensions and has an inside dimension that is about 160×160 mm square for use with a 15 mm wide seal, thereby providing for about 4 mm of overlap between the edge of the seal and the end of the tie layer to provide stress relief in the seal edge. The tie layer frame is heat bonded to the PVDF membrane using an impulse heat sealer. The pair of tie layer bonded membranes are then heat sealed together around their periphery using a pair of custom fabricated constant heat aluminum dies having a tube sealing groove, as previously described, to yield an inner collapsible blood container. A pair of Conwed Thermanet part #R03470 polymer integrating mesh sheets are cut to approximately 10 mm larger than the inner collapsible blood bag periphery and placed on both sides of the inner collapsible blood bag with the adhesive sides of the polymer integrating mesh in contact with the inner collapsible blood bag. The assembly is placed between a pair of aluminum plates and heated to about 93-110° C., up to as much as 120° C., for about 3-15 minutes to melt the adhesive and allow it to flow into the pores of the PVDF membrane directly underneath the integrating polymer mesh, and also around the periphery of the inner collapsible blood bag where the polymer integrating mesh is in contact with itself, thereby providing a strong mechanical bond. The assembly is allowed to cool below about 50° C. before removing the plates. 
     Example 12: Effect of Spacer  110  on Deoxygenation Rates 
     Inner collapsible blood bag and oxygen depletion device are according the examples above with and without a spacer  110  according to the present disclosure. As shown in  FIG.  16   , incorporation of a spacer  110  significantly increases the rate of oxygen depletion. 
     While the invention has been described with reference to particular embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the invention without departing from the scope of the invention. 
     Therefore, it is intended that the invention not be limited to the particular embodiments disclosed as the best mode contemplated for carrying out this invention, but that the invention will include all embodiments falling within the scope and spirit of the appended claims.