Patent Publication Number: US-2020291374-A1

Title: Novel metalloproteases

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application is a Divisional of U.S. Ser. No. 15/835,551 filed Dec. 8, 2017, which is Continuation of U.S. Ser. No. 14/893,473, filed Nov. 23, 2015, which is a 371 of International Patent Application No. PCT/US2014/039928, filed May 29, 2014, which claims benefit of priority from International patent applications Serial No. PCT/CN2013/076419; Serial No. PCT/CN2013/076387; Serial No. PCT/CN2013/076401; Serial No. PCT/CN2013/076406; Serial No. PCT/CN2013/076414; Serial No. PCT/CN2013/076384; Serial No. PCT/CN2013/076398; and Serial No. PCT/CN2013/076415; all filed on 29 May 2013, the contents of which are incorporated herein by reference in their entirety. 
    
    
     SEQUENCE LISTING 
     The sequence listing submitted via EFS, in compliance with 37 C.F.R. § 1.52(e), is incorporated herein by reference. The sequence listing text file submitted via EFS contains the file “20200522_NB40167USPCD2_SeqLst” created on May 22, 2020, which is 207 kilo bytes in size. 
     FIELD OF THE INVENTION 
     The present disclosure relates to proteases and variants thereof. Compositions containing the proteases are suitable for use in cleaning, food and feed as well as in a variety of other industrial applications. 
     BACKGROUND 
     Metalloproteases (MPs) are among the hydrolases that mediate nucleophilic attack on peptide bonds using a water molecule coordinated in the active site. In their case, a divalent ion, such as zinc, activates the water molecule. This metal ion is held in place by amino acid ligands, usually 3 in number. The clan MA consists of zinc-dependent MPs in which two of the zinc ligands are the histidines in the motif: HisGluXXHis (SEQ ID NO: 41). This Glu is the catalytic residue. These are two domain proteases with the active site between the domains. In subclan MA(E), also known as Glu-zincins, the 3 rd  ligand is a Glu located C-terminal to the HDXXH (SEQ ID NO: 42) motif. Members of the families: M1, 3, 4, 13, 27 and 34 are all secreted proteases, almost exclusively from bacteria (Rawlings and Salvessen (2013) Handbook of Proteolytic Enzymes, Elsevier Press). They are generally active at elevated temperatures and this stability is attributed to calcium binding. Thermolysin-like proteases are found in the M4 family as defined by MEROPS (Rawlings et al., (2012) Nucleic Acids Res 40:D343-D350). Although proteases have long been known in the art of industrial enzymes, there remains a need for novel proteases that are suitable for particular conditions and uses. 
     SUMMARY 
     The present disclosure provides novel metalloprotease enzymes, nucleic acids encoding the same, and compositions and methods related to the production and use thereof. 
     In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 3. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, Alicyclobacillaceae, Lactobacillaceae, or a  Bacillus, Alicyclobacillus, Geobacillus, Exiguobacterium, Lactobacillus , or  Paenibacillus  spp., such as  Paenibacillus polymyxa . In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the Pseudococcidae, or a  Planococcus  spp., such as  Planococcus donghaensis . In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 9.5. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 30° C. and 70° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition. 
     In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 8. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, or Brevibacillaceae, or a  Bacillus, Brevibacillus , or  Paenibacillus  spp., such as  Paenibacillus  sp. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from  Brevibacillus  sp. In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 10. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 35° C. and 70° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition. 
     In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 13. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, or Brevibacillaceae, or a  Bacillus, Geobacillus, Brevibacillus , or  Paenibacillus  spp., such as  Paenibacillus humicus . In some embodiments, the invention is any of the above, wherein said polypeptide is derived from  Bacillus polymyxa . In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 9.5. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 35° C. and 70° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition. 
     In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 18. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, or Brevibacillaceae, or a  Bacillus, Geobacillus, Brevibacillus , or  Paenibacillus  spp., such as  Paenibacillus ehimensis . In some embodiments, the invention is any of the above, wherein said polypeptide is derived from  Brevibacillus  sp. In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 10.5. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 45° C. and 75° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition. 
     In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 23. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, Alicyclobacillaceae, Lactobacillaceae, or a  Bacillus, Geobacillus, Alicyclobacillus, Brevibacillus, Paenibacillus , or  Lactobacillus  spp., such as  Paenibacillus barcinonensis . In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the family Pseudococcidae, or a  Planococcus  spp., such as  Planococcus donghaensis . In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 10. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 35° C. and 65° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition. 
     In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 28. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, or a  Bacillus, Brevibacillus, Paenibacillus , or  Lactobacillus  spp., such as  Paenibacillus polymyxa . In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the family Pseudococcidae, or a  Planococcus  spp., such as  Planococcus donghaensis . In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 9.5. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 30° C. and 65° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition. 
     In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 33. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, or a  Bacillus, Geobacillus, Brevibacillus , or  Paenibacillus  spp., such as  Paenibacillus hunanensis . In some embodiments, the invention is any of the above, wherein said polypeptide is derived from  Bacillus polymyxa . In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 4.5 and 9.0. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 35° C. and 70° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition. 
     In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 38. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, Lactobacillaceae, or a  Bacillus, Brevibacillus, Lactobacillus, Paenibacillus , or  Geobacillus  spp., such as  Paenibacillus amylolyticus . In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5.5 and 10. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 35° C. and 65° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition. 
     In some embodiments, the invention is a composition comprising any of the above, such as a cleaning or detergent composition. In some embodiments, the composition further comprises a surfactant, at least one calcium ion and/or zinc ion, at least one stabilizer, at least one bleaching agent, and can contain phosphate, or be phosphate-free. In some embodiments, the composition further comprises one or more additional enzymes or enzyme derivatives selected from the group consisting of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, and combinations thereof. In some embodiments, the composition is formulated at a pH of from about 5.5 to about 8.5. In some embodiments, the invention is a method of cleaning using any of the above polypeptides or compositions. In some embodiments, the invention is a textile processing composition, animal feed composition, leather processing composition, or feather processing composition. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1.1  provides a plasmid map of pGX085 (aprE-PspPro3), described in Example 1.2. 
         FIG. 1.2  provides a dose response curve of PspPro3 in the azo-casein assay. 
         FIG. 1.3  provides the pH profile of PspPro3. 
         FIG. 1.4  provides the temperature profile of PspPro3. 
         FIG. 1.5A  shows dose response for cleaning of PA-S-38 microswatches by PspPro3 protein in ADW detergent at pH 6 and 8. 
         FIG. 1.5B  shows dose response for cleaning of PA-S-38 microswatches shows by PspPro3 protein in ADW detergent at pH 6 and 8 in the presence of bleach. 
         FIG. 1.6  shows cleaning performance of PspPro3 protein in liquid laundry detergent. 
         FIG. 1.7  (SEQ ID NOS: 3, 44, and 45, respectively) shows alignment of PspPro3 with other protein homologs. 
         FIG. 1.8  provides the phylogenetic tree for PspPro3 and its homologs. 
         FIG. 2.1  provides a plasmid map of pGX084 (aprE-PspPro2), described in Example 2.2. 
         FIG. 2.2  provides a dose response curve of PspPro2 in the azo-casein assay. 
         FIG. 2.3  provides the pH profile of purified PspPro2. 
         FIG. 2.4  provides the temperature profile of purified PspPro2. 
         FIG. 2.5A  shows dose response for cleaning performance of PspPro2 at pH 6 in AT dish detergent with bleach. 
         FIG. 2.5B  shows dose response for cleaning performance of purified PspPro2 at pH 8 in AT detergent with bleach. 
         FIG. 2.6A  shows cleaning performance of PspPro2 protein in liquid laundry detergent. 
         FIG. 2.6B  shows cleaning performance of PspPro2 protein in powder laundry detergent. 
         FIG. 2.7  (SEQ ID NOS: 8, 46, and 45, respectively) shows alignment of PspPro2 with other protein homologs. 
         FIG. 2.8  provides the phylogenetic tree for PspPro2 and its homologs. 
         FIG. 3.1  provides a plasmid map of pGX150 (aprE-PhuPro2), described in Example 3.2. 
         FIG. 3.2  provides a dose response curve of PhuPro2 in the azo-casein assay. 
         FIG. 3.3  provides the pH profile of purified PhuPro2. 
         FIG. 3.4  provides the temperature profile of purified PhuPro2. 
         FIG. 3.5A  shows dose response for c leaning performance of PhuPro2 in AT dish detergent at pH 6. 
         FIG. 3.5B  shows dose response for cleaning performance of PhuPro2 in AT dish detergent at pH 8. 
         FIG. 3.6  (SEQ ID NOS: 13, 47 and 45, respectively) shows alignment of PhuPro2 with other protein homologs. 
         FIG. 3.7  provides the phylogenetic tree for PhuPro2 and its homologs. 
         FIG. 4.1  provides a plasmid map of pGX148 (aprE-PehPro1), described in Example 4.2. 
         FIG. 4.2  provides a dose response curve of PehPro1 in the azo-casein assay. 
         FIG. 4.3  provides the pH profile of purified PehPro1. 
         FIG. 4.4  provides the temperature profile of purified PehPro1. 
         FIG. 4.5A  shows dose response for cleaning performance of PehPro1 at pH 6 in AT dish detergent with bleach. 
         FIG. 4.5B  shows dose response for cleaning performance of purified PehPro1 at pH 8 in AT detergent with bleach. 
         FIG. 4.6  (SEQ ID NOS: 18, 48, and 45, respectively) shows alignment of PehPro1 with other protein homologs. 
         FIG. 4.7  provides the phylogenetic tree for PehPro1 and its homologs. 
         FIG. 5.1  provides a plasmid map of pGX147 (aprE-PbaPro1), described in Example 5.2. 
         FIG. 5.2  provides a dose response curve of PbaPro1 in the azo-casein assay. 
         FIG. 5.3  provides the pH profile of purified PbaPro1. 
         FIG. 5.4  provides the temperature profile of purified PbaPro1. 
         FIG. 5.5A  shows dose response for cleaning of PA-S-38 microswatches by PbaPro1protein in ADW detergent at pH 6. 
         FIG. 5.5B  shows dose response for cleaning of PA-S-38 microswatches shows by PbaPro1protein in ADW detergent at pH 8. 
         FIG. 5.6  (SEQ ID NOS: 23, 49, and 45, respectively) shows the alignment of PbaPro1 with protease homologs. 
         FIG. 5.7  provides the phylogenetic tree for PbaPro1 and its homologs. 
         FIG. 6.1  provides a plasmid map of pGX138 (aprE-PpoPro1), described in Example 6.2. 
         FIG. 6.2  provides a dose response curve of PpoPro1 in the azo-casein assay. 
         FIG. 6.3  provides the pH profile of purified PpoPro1. 
         FIG. 6.4  provides the temperature profile of purified PpoPro1. 
         FIG. 6.5A  shows dose response for cleaning of PA-S-38 microswatches by PpoPro1protein in ADW detergent at pH 6 in the presence of bleach. 
         FIG. 6.5B  shows dose response for cleaning of PA-S-38 microswatches shows by PpoPro1protein in ADW detergent at pH 8 in the presence of bleach. 
         FIG. 6.6  (SEQ ID NOS: 28, 50, and 45, respectively) shows the alignment of PpoPro1 with protease homologs. 
         FIG. 6.7  provides the phylogenetic tree for PpoPro1 and its homologs. 
         FIG. 7.1  provides a plasmid map of pGX149 (aprE-PhuPro1), described in Example 7.2. 
         FIG. 7.2  provides a dose response curve of PhuPro1 in the azo-casein assay. 
         FIG. 7.3  provides the pH profile of purified PhuPro1. 
         FIG. 7.4  provides the temperature profile of purified PhuPro1. 
         FIG. 7.5A  shows dose response for cleaning of PA-S-38 microswatches by PhuPro1 protein in ADW detergent at pH 6. 
         FIG. 7.5B  shows dose response for cleaning of PA-S-38 microswatches shows by Phu Pro1protein in ADW detergent at pH 8. 
         FIG. 7.6  (SEQ ID NOS: 33, 51, and 45, respectively) shows alignment of PhuPro1 with other protein homologs. 
         FIG. 7.7  provides the phylogenetic tree for PhuPro1 and its homologs. 
         FIGS. 7.8A and 7.8B  show cleaning performances of PhuPro1 and Purafect® Prime HA proteases. 
         FIG. 8.1  provides a plasmid map of pGX146 (aprE-PamPro1), described in Example 8.2. 
         FIG. 8.2  provides a dose response curve of PamPro1 in the azo-casein assay. 
         FIG. 8.3  provides the pH profile of purified PamPro1. 
         FIG. 8.4  provides the temperature profile of purified PamPro1. 
         FIG. 8.5A  shows dose response for cleaning of PA-S-38 microswatches by PamPro1 protein in ADW detergent at pH 6. 
         FIG. 8.5B  shows dose response for cleaning of PA-S-38 microswatches shows by PamPro1 protein in ADW detergent at pH 8. 
         FIG. 8.6  (SEQ ID NOS: 38, 52, and 45, respectively) shows the alignment of PamPro1 with protease homologs. 
         FIG. 8.7  provides the phylogenetic tree for PamPro1 and its homologs. 
         FIGS. 9.1A  thru  9 . 1 D (SEQ ID NOS: 53-62, 38, 23, 13, 63, 8, 28, 64, 3, 18, 33, 65-68, respectively) show the alignment of the various  Paenibacillus  metalloproteases with other bacterial metalloprotease homologs. 
         FIG. 9.2  provides the phylogenetic tree of the various  Paenibacillus  metalloproteases with other bacterial metalloprotease homologs. 
     
    
    
     DETAILED DESCRIPTION 
     The present invention provides novel metalloprotease enzymes, especially enzymes useful for detergent compositions cloned from various  Paenibacillus  sp. The compositions and methods are based, in part, on the observation that the novel metalloproteases of the present invention have proteolytic activity in the presence of detergent compositions. This feature makes metalloproteases of the present invention particularly well suited to and useful in a variety of cleaning applications where the enzyme can hydrolyze polypeptides in the presence of surfactants and other components found in detergent compositions. The invention includes compositions comprising at least one of the novel metalloprotease enzymes set forth herein. Some such compositions comprise detergent compositions. The metalloprotease enzymes of the present invention can be combined with other enzymes useful in detergent compositions. The invention also provides methods of cleaning using metalloprotease enzymes of the present invention. 
     Definitions and Abbreviations 
     Unless otherwise indicated, the practice of the present invention involves conventional techniques commonly used in molecular biology, protein engineering, microbiology, and recombinant DNA technology, which are within the skill of the art. Such techniques are known to those of skill in the art and are described in numerous texts and reference works well known to those of skill in the art. All patents, patent applications, articles and publications mentioned herein, both supra and infra, are hereby expressly incorporated herein by reference. 
     Unless defined otherwise herein, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Many technical dictionaries are known to those of skill in the art. Although any methods and materials similar or equivalent to those described herein find use in the practice of the present invention, some suitable methods and materials are described herein. Accordingly, the terms defined immediately below are more fully described by reference to the Specification as a whole. Also, as used herein, the singular “a”, “an” and “the” includes the plural reference unless the context clearly indicates otherwise. Unless otherwise indicated, nucleic acids are written left to right in 5′ to 3′ orientation; amino acid sequences are written left to right in amino to carboxy orientation, respectively. It is to be understood that this invention is not limited to the particular methodology, protocols, and reagents described, as these may vary, depending upon the context they are used by those of skill in the art. 
     Furthermore, the headings provided herein are not limitations of the various aspects or embodiments of the invention. 
     It is intended that every maximum numerical limitation given throughout this specification includes every lower numerical limitation, as if such lower numerical limitations were expressly written herein. Every minimum numerical limitation given throughout this specification will include every higher numerical limitation, as if such higher numerical limitations were expressly written herein. Every numerical range given throughout this specification will include every narrower numerical range that falls within such broader numerical range, as if such narrower numerical ranges were all expressly written herein. 
     As used herein, the terms “protease” and “proteinase” refer to an enzyme that has the ability to break down proteins and peptides. A protease has the ability to conduct “proteolysis,” by hydrolysis of peptide bonds that link amino acids together in a peptide or polypeptide chain forming the protein. This activity of a protease as a protein-digesting enzyme is referred to as “proteolytic activity.” Many well known procedures exist for measuring proteolytic activity (See e.g., Kalisz, “Microbial Proteinases,” In: Fiechter (ed.), Advances in Biochemical Engineering/Biotechnology, (1988)). For example, proteolytic activity may be ascertained by comparative assays which analyze the respective protease&#39;s ability to hydrolyze a suitable substrate. Exemplary substrates useful in the analysis of protease or proteolytic activity, include, but are not limited to, di-methyl casein (Sigma C-9801), bovine collagen (Sigma C-9879), bovine elastin (Sigma E-1625), and bovine keratin (ICN Biomedical 902111). Colorimetric assays utilizing these substrates are well known in the art (See e.g., WO 99/34011 and U.S. Pat. No. 6,376,450, both of which are incorporated herein by reference). The pNA peptidyl assay (See e.g., Del Mar et al., Anal. Biochem. 99:316-320 [1979]) also finds use in determining the active enzyme concentration. This assay measures the rate at which p-nitroaniline is released as the enzyme hydrolyzes a soluble synthetic substrate, such as succinyl-alanine-alanine-proline-phenylalanine-p-nitroanilide (suc-AAPF-pNA)(SEQ ID NO: 43). The rate of production of yellow color from the hydrolysis reaction is measured at 410 nm on a spectrophotometer and is proportional to the active enzyme concentration. In addition, absorbance measurements at 280 nanometers (nm) can be used to determine the total protein concentration in a sample of purified protein. The activity on substrate/protein concentration gives the enzyme specific activity. 
     As used herein, the term “variant polypeptide” refers to a polypeptide comprising an amino acid sequence that differs in at least one amino acid residue from the amino acid sequence of a parent or reference polypeptide (including but not limited to wild-type polypeptides). 
     As used herein, “the genus  Bacillus ” includes all species within the genus “ Bacillus ,” as known to those of skill in the art, including but not limited to  B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. clausii, B. halodurans, B. megaterium, B. coagulans, B. circulans , and  B. thuringiensis . It is recognized that the genus  Bacillus  continues to undergo taxonomical reorganization. Thus, it is intended that the genus include species that have been reclassified, including but not limited to such organisms as  B. stearothermophilus , which is now named “ Geobacillus stearothermophilus .” The production of resistant endospores under stressful environmental conditions is considered the defining feature of the genus  Bacillus , although this characteristic also applies to the recently named  Alicyclobacillus , Amphibacillus, Aneurinibacillus,  Paenibacillus, Brevibacillus, Filobacillus, Gracilibacillus, Halobacillus, Paenibacillus, Salibacillus, Thermobacillus, Ureibacillus , and  Virgibacillus.    
     The terms “polynucleotide” and “nucleic acid,” which are used interchangeably herein, refer to a polymer of any length of nucleotide monomers covalently bonded in a chain. DNA (deoxyribonucleic acid), a polynucleotide comprising deoxyribonucleotides, and RNA (ribonucleic acid), a polymer of ribonucleotides, are examples of polynucleotides or nucleic acids having distinct biological function. Polynucleotides or nucleic acids include, but are not limited to, a single-, double- or triple-stranded DNA, genomic DNA, cDNA, RNA, DNA-RNA hybrid, or a polymer comprising purine and pyrimidine bases, or other natural, chemically, biochemically modified, non-natural or derivatized nucleotide bases. The following are non-limiting examples of polynucleotides: genes, gene fragments, chromosomal fragments, expressed sequence tag(s) (EST(s)), exons, introns, messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), ribozymes, complementary DNA (cDNA), recombinant polynucleotides, branched polynucleotides, plasmids, vectors, isolated DNA of any sequence, isolated RNA of any sequence, nucleic acid probes, and primers. 
     As used herein, the term “mutation” refers to changes made to a reference amino acid or nucleic acid sequence. It is intended that the term encompass substitutions, insertions and deletions. 
     As used herein, the term “vector” refers to a nucleic acid construct used to introduce or transfer nucleic acid(s) into a target cell or tissue. A vector is typically used to introduce foreign DNA into a cell or tissue. Vectors include plasmids, cloning vectors, bacteriophages, viruses (e.g., viral vector), cosmids, expression vectors, shuttle vectors, and the like. A vector typically includes an origin of replication, a multicloning site, and a selectable marker. The process of inserting a vector into a target cell is typically referred to as transformation. The present invention includes, in some embodiments, a vector that comprises a DNA sequence encoding a metalloprotease polypeptide (e.g., precursor or mature metalloprotease polypeptide) that is operably linked to a suitable prosequence (e.g., secretory, signal peptide sequence, etc.) capable of effecting the expression of the DNA sequence in a suitable host, and the folding and translocation of the recombinant polypeptide chain. 
     As used herein, the term “expression cassette,” “expression plasmid” or “expression vector” refers to a nucleic acid construct or vector generated recombinantly or synthetically for the expression of a nucleic acid of interest in a target cell. An expression vector or expression cassette typically comprises a promoter nucleotide sequence that drives expression of the foreign nucleic acid. The expression vector or cassette also typically includes any other specified nucleic acid elements that permit transcription of a particular nucleic acid in a target cell. A recombinant expression cassette can be incorporated into a plasmid, chromosome, mitochondrial DNA, plastid DNA, virus, or nucleic acid fragment. Many prokaryotic and eukaryotic expression vectors are commercially available. 
     In some embodiments, the ends of the sequence are closed such that the DNA construct forms a closed circle. The nucleic acid sequence of interest, which is incorporated into the DNA construct, using techniques well known in the art, may be a wild-type, mutant, or modified nucleic acid. In some embodiments, the DNA construct comprises one or more nucleic acid sequences homologous to the host cell chromosome. In other embodiments, the DNA construct comprises one or more non-homologous nucleotide sequences. Once the DNA construct is assembled in vitro, it may be used, for example, to: 1) insert heterologous sequences into a desired target sequence of a host cell; and/or 2) mutagenize a region of the host cell chromosome (i.e., replace an endogenous sequence with a heterologous sequence); 3) delete target genes; and/or 4) introduce a replicating plasmid into the host. “DNA construct” is used interchangeably herein with “expression cassette.” 
     As used herein, a “plasmid” refers to an extrachromosomal DNA molecule which is capable of replicating independently from the chromosomal DNA. A plasmid is double stranded (ds) and may be circular and is typically used as a cloning vector. 
     As used herein in the context of introducing a nucleic acid sequence into a cell, the term “introduced” refers to any method suitable for transferring the nucleic acid sequence into the cell. Such methods for introduction include but are not limited to protoplast fusion, transfection, transformation, electroporation, conjugation, and transduction (See e.g., Ferrari et al., “Genetics,” in Hardwood et al. (eds.),  Bacillus , Plenum Publishing Corp., pp. 57-72 [1989]). 
     Transformation refers to the genetic alteration of a cell which results from the uptake, optional genomic incorporation, and expression of genetic material (e.g., DNA). 
     As used herein, a nucleic acid is “operably linked” with another nucleic acid sequence when it is placed into a functional relationship with another nucleic acid sequence. For example, a promoter or enhancer is operably linked to a nucleotide coding sequence if the promoter affects the transcription of the coding sequence. A ribosome binding site may be operably linked to a coding sequence if it is positioned so as to facilitate translation of the coding sequence. Typically, “operably linked” DNA sequences are contiguous. However, enhancers do not have to be contiguous. Linking is accomplished by ligation at convenient restriction sites. If such sites do not exist, synthetic oligonucleotide adaptors or linkers may be used in accordance with conventional practice. 
     As used herein the term “gene” refers to a polynucleotide (e.g., a DNA segment), that encodes a polypeptide and includes regions preceding and following the coding regions as well as intervening sequences (introns) between individual coding segments (exons). 
     As used herein, “recombinant” when used with reference to a cell typically indicates that the cell has been modified by the introduction of a foreign nucleic acid sequence or that the cell is derived from a cell so modified. For example, a recombinant cell may comprise a gene not found in identical form within the native (non-recombinant) form of the cell, or a recombinant cell may comprise a native gene (found in the native form of the cell) but which has been modified and re-introduced into the cell. A recombinant cell may comprise a nucleic acid endogenous to the cell that has been modified without removing the nucleic acid from the cell; such modifications include those obtained by gene replacement, site-specific mutation, and related techniques known to those of ordinary skill in the art. Recombinant DNA technology includes techniques for the production of recombinant DNA in vitro and transfer of the recombinant DNA into cells where it may be expressed or propagated, thereby producing a recombinant polypeptide. “Recombination,” “recombining,” and “recombined” of polynucleotides or nucleic acids refer generally to the assembly or combining of two or more nucleic acid or polynucleotide strands or fragments to generate a new polynucleotide or nucleic acid. The recombinant polynucleotide or nucleic acid is sometimes referred to as a chimera. A nucleic acid or polypeptide is “recombinant” when it is artificial or engineered. 
     A nucleic acid or polynucleotide is said to “encode” a polypeptide if, in its native state or when manipulated by methods known to those of skill in the art, it can be transcribed and/or translated to produce the polypeptide or a fragment thereof. The anti-sense strand of such a nucleic acid is also said to encode the sequence. 
     “Host strain” or “host cell” refers to a suitable host for an expression vector comprising a DNA sequence of interest. 
     A “protein” or “polypeptide” comprises a polymeric sequence of amino acid residues. The terms “protein” and “polypeptide” are used interchangeably herein. The single and 3-letter code for amino acids as defined in conformity with the IUPAC-IUB Joint Commission on Biochemical Nomenclature (JCBN) is used through out this disclosure. The single letter X refers to any of the twenty amino acids. It is also understood that a polypeptide may be coded for by more than one nucleotide sequence due to the degeneracy of the genetic code. Mutations can be named by the one letter code for the parent amino acid, followed by a position number and then the one letter code for the variant amino acid. For example, mutating glycine (G) at position 87 to serine (S) is represented as “G087S” or “G87S”. Mutations can also be named by using the three letter code for an amino acid followed by its position in the polypeptide chain as counted from the N-terminus; for example, Ala10 for alanine at position 10. Multiple mutations are indicated by inserting a “-” between the mutations. Mutations at positions 87 and 90 are represented as either “G087S-A090Y” or “G87S-A90Y” or “G87S+A90Y” or “G087S+A090Y”. For deletions, the one letter code “Z” is used. For an insertion relative to the parent sequence, the one letter code “Z” is on the left side of the position number. For a deletion, the one letter code “Z” is on the right side of the position number. For insertions, the position number is the position number before the inserted amino acid(s), plus 0.01 for each amino acid. For example, an insertion of three amino acids alanine (A), serine (S) and tyrosine (Y) between position 87 and 88 is shown as “Z087.01A-Z087.02S-Z087.03Y.” Thus, combining all the mutations above plus a deletion at position 100 is: “G087S-Z087.01A-Z087.02S-Z087.03Y-A090Y-A100Z.” When describing modifications, a position followed by amino acids listed in parentheses indicates a list of substitutions at that position by any of the listed amino acids. For example, 6(L,I) means position 6 can be substituted with a leucine or isoleucine. 
     A “prosequence” or “propetide sequence” refers to an amino acid sequence between the signal peptide sequence and mature protease sequence that is necessary for the proper folding and secretion of the protease; they are sometimes referred to as intramolecular chaperones. Cleavage of the prosequence or propeptide sequence results in a mature active protease. Bacterial metalloproteases are often expressed as pro-enzymes. 
     The term “signal sequence” or “signal peptide” refers to a sequence of amino acid residues that may participate in the secretion or direct transport of the mature or precursor form of a protein. The signal sequence is typically located N-terminal to the precursor or mature protein sequence. The signal sequence may be endogenous or exogenous. A signal sequence is normally absent from the mature protein. A signal sequence is typically cleaved from the protein by a signal peptidase after the protein is transported. 
     The term “mature” form of a protein, polypeptide, or peptide refers to the functional form of the protein, polypeptide, or peptide without the signal peptide sequence and propeptide sequence. 
     The term “precursor” form of a protein or peptide refers to a mature form of the protein having a prosequence operably linked to the amino or carbonyl terminus of the protein. The precursor may also have a “signal” sequence operably linked to the amino terminus of the prosequence. The precursor may also have additional polypeptides that are involved in post-translational activity (e.g., polypeptides cleaved therefrom to leave the mature form of a protein or peptide). 
     The term “wild-type” in reference to an amino acid sequence or nucleic acid sequence indicates that the amino acid sequence or nucleic acid sequence is native or naturally occurring sequence. As used herein, the term “naturally-occurring” refers to anything (e.g., proteins, amino acids, or nucleic acid sequences) that are found in nature. 
     As used herein, the term “non-naturally occurring” refers to anything that is not found in nature (e.g., recombinant nucleic acids and protein sequences produced in the laboratory), as modification of the wild-type sequence. 
     As used herein with regard to amino acid residue positions, “corresponding to” or “corresponds to” or “corresponds” refers to an amino acid residue at the enumerated position in a protein or peptide, or an amino acid residue that is analogous, homologous, or equivalent to an enumerated residue in a protein or peptide. As used herein, “corresponding region” generally refers to an analogous position in a related proteins or a reference protein. 
     The terms “derived from” and “obtained from” refer to not only a protein produced or producible by a strain of the organism in question, but also a protein encoded by a DNA sequence isolated from such strain and produced in a host organism containing such DNA sequence. Additionally, the term refers to a protein which is encoded by a DNA sequence of synthetic and/or cDNA origin and which has the identifying characteristics of the protein in question. To exemplify, “proteases derived from  Bacillus ” refers to those enzymes having proteolytic activity which are naturally produced by  Bacillus , as well as to serine proteases like those produced by  Bacillus  sources but which through the use of genetic engineering techniques are produced by non- Bacillus  organisms transformed with a nucleic acid encoding the serine proteases. 
     The term “identical” in the context of two nucleic acids or polypeptidesequences refers to the residues in the two sequences that are the same when aligned for maximum correspondence, as measured using one of the following sequence comparison or analysis algorithms. 
     As used herein, “homologous genes” refers to a pair of genes from different, but usually related species, which correspond to each other and which are identical or very similar to each other. The term encompasses genes that are separated by speciation (i.e., the development of new species) (e.g., orthologous genes), as well as genes that have been separated by genetic duplication (e.g., paralogous genes). 
     As used herein, “% identity or percent identity” refers to sequence similarity. Percent identity may be determined using standard techniques known in the art (See e.g., Smith and Waterman, Adv. Appl. Math. 2:482 [1981]; Needleman and Wunsch, J. Mol. Biol. 48:443 [1970]; Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444 [1988]; software programs such as GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package (Genetics Computer Group, Madison, Wis.); and Devereux et al., Nucl. Acid Res. 12:387-395 [1984]). One example of a useful algorithm is PILEUP. PILEUP creates a multiple sequence alignment from a group of related sequences using progressive, pair-wise alignments. It can also plot a tree showing the clustering relationships used to create the alignment. PILEUP uses a simplification of the progressive alignment method of Feng and Doolittle (See, Feng and Doolittle, J. Mol. Evol. 35:351-360 [1987]). The method is similar to that described by Higgins and Sharp (See, Higgins and Sharp, CABIOS 5:151-153 [1989]). Useful PILEUP parameters include a default gap weight of 3.00, a default gap length weight of 0.10, and weighted end gaps. Other useful algorithm is the BLAST algorithms described by Altschul et al., (See, Altschul et al., J. Mol. Biol. 215:403-410 [1990]; and Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873-5787 [1993]). The BLAST program uses several search parameters, most of which are set to the default values. 
     The NCBI BLAST algorithm finds the most relevant sequences in terms of biological similarity but is not recommended for query sequences of less than 20 residues (Altschul, S F et al. (1997) Nucleic Acids Res. 25:3389-3402 and Schaffer, A A et al. (2001) Nucleic Acids Res. 29:2994-3005). Example default BLAST parameters for a nucleic acid sequence searches are:
         Neighboring words threshold: 11   E-value cutoff: 10   Scoring Matrix: NUC.3.1 (match=1, mismatch=−3)   Gap Opening: 5   Gap Extension: 2
 
and the following parameters for amino acid sequence searches:
   Word size: 3   E-value cutoff: 10   Scoring Matrix: BLOSUM62   Gap Opening: 11   Gap extension: 1       

     A percent (%) amino acid sequence identity value is determined by the number of matching identical residues divided by the total number of residues of the “reference” sequence including any gaps created by the program for optimal/maximum alignment. If a sequence is 90% identical to SEQ ID NO: A, SEQ ID NO: A is is the “reference” sequence. BLAST algorithms refer the “reference” sequence as “query” sequence. 
     The CLUSTAL W algorithm is another example of a sequence alignment algorithm. See Thompson et al. (1994) Nucleic Acids Res. 22:4673-4680. Default parameters for the CLUSTAL W algorithm are:
         Gap opening penalty: 10.0   Gap extension penalty: 0.05   Protein weight matrix: BLOSUM series   DNA weight matrix: IUB   Delay divergent sequences %: 40   Gap separation distance: 8   DNA transitions weight: 0.50   List hydrophilic residues: GPSNDQEKR   Use negative matrix: OFF   Toggle Residue specific penalties: ON   Toggle hydrophilic penalties: ON   Toggle end gap separation penalty OFF.       

     In CLUSTAL algorithms, deletions occurring at either terminus are included. For example, a variant with five amino acid deletion at either terminus (or within the polypeptide) of a polypeptide of 500 amino acids would have a percent sequence identity of 99% (495/500 identical residues×100) relative to the “reference” polypeptide. Such a variant would be encompassed by a variant having “at least 99% sequence identity” to the polypeptide. 
     A polypeptide of interest may be said to be “substantially identical” to a reference polypeptide if the polypeptide of interest comprises an amino acid sequence having at least about 60%, least about 65%, least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least about 99.5% sequence identity to the amino acid sequence of the reference polypeptide. The percent identity between two such polypeptides can be determined manually by inspection of the two optimally aligned polypeptide sequences or by using software programs or algorithms (e.g., BLAST, ALIGN, CLUSTAL) using standard parameters. One indication that two polypeptides are substantially identical is that the first polypeptide is immunologically cross-reactive with the second polypeptide. Typically, polypeptides that differ by conservative amino acid substitutions are immunologically cross-reactive. Thus, a polypeptide is substantially identical to a second polypeptide, for example, where the two peptides differ only by a conservative amino acid substitution or one or more conservative amino acid substitutions. 
     A nucleic acid of interest may be said to be “substantially identical” to a reference nucleic acid if the nucleic acid of interest comprises a nucleotide sequence having least about 60%, least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least about 99.5% sequence identity to the nucleotide sequence of the reference nucleic acid. The percent identity between two such nucleic acids can be determined manually by inspection of the two optimally aligned nucleic acid sequences or by using software programs or algorithms (e.g., BLAST, ALIGN, CLUSTAL) using standard parameters. One indication that two nucleic acid sequences are substantially identical is that the two nucleic acid molecules hybridize to each other under stringent conditions (e.g., within a range of medium to high stringency). 
     A nucleic acid or polynucleotide is “isolated” when it is at least partially or completely separated from other components, including but not limited to for example, other proteins, nucleic acids, cells, etc. Similarly, a polypeptide, protein or peptide is “isolated” when it is at least partially or completely separated from other components, including but not limited to for example, other proteins, nucleic acids, cells, etc. On a molar basis, an isolated species is more abundant than are other species in a composition. For example, an isolated species may comprise at least about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, or about 100% (on a molar basis) of all macromolecular species present. Preferably, the species of interest is purified to essential homogeneity (i.e., contaminant species cannot be detected in the composition by conventional detection methods). Purity and homogeneity can be determined using a number of techniques well known in the art, such as agarose or polyacrylamide gel electrophoresis of a nucleic acid or a protein sample, respectively, followed by visualization upon staining. If desired, a high-resolution technique, such as high performance liquid chromatography (HPLC) or a similar means can be utilized for purification of the material. 
     “Hybridization” refers to the process by which one strand of nucleic acid forms a duplex with, i.e., base pairs with, a complementary strand. A nucleic acid sequence is considered to be “selectively hybridizable” to a reference nucleic acid sequence if the two sequences specifically hybridize to one another under moderate to high stringency hybridization and wash conditions. Hybridization conditions are based on the melting temperature (Tm) of the nucleic acid binding complex or probe. For example, “maximum stringency” typically occurs at about Tm−5° C. (5° below the Tm of the probe); “high stringency” at about 5-10° C. below the Tm; “intermediate stringency” at about 10-20° C. below the Tm of the probe; and “low stringency” at about 20-25° C. below the Tm. Functionally, maximum stringency conditions can be used to identify sequences having strict identity or near-strict identity with the hybridization probe; while intermediate or low stringency hybridization can be used to identify or detect polynucleotide sequence homologs. 
     Moderate and high stringency hybridization conditions are well known in the art. Stringent hybridization conditions are exemplified by hybridization under the following conditions: 65° C. and 0.1×SSC (where 1×SSC=0.15 M NaCl, 0.015 M Na3 citrate, pH 7.0). Hybridized, duplex nucleic acids are characterized by a melting temperature (T m ), where one half of the hybridized nucleic acids are unpaired with the complementary strand. Mismatched nucleic acids within the duplex lower the T m . Very stringent hybridization conditions involve 68° C. and 0.1×SSC. A nucleic acid encoding a variant metalloprotease can have a T m  reduced by 1° C.-3° C. or more compared to a duplex formed between the nucleic acid and its identical complement. 
     Another example of high stringency conditions includes hybridization at about 42° C. in 50% formamide, 5×SSC, 5×Denhardt&#39;s solution, 0.5% SDS and 100 μg/ml denatured carrier DNA followed by washing two times in 2×SSC and 0.5% SDS at room temperature and two additional times in 0.1×SSC and 0.5% SDS at 42° C. An example of moderate stringent conditions include an overnight incubation at 37° C. in a solution comprising 20% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5×Denhardt&#39;s solution, 10% dextran sulfate and 20 mg/ml denatured sheared salmon sperm DNA, followed by washing the filters in 1×SSC at about 37-50° C. Those of skill in the art know how to adjust the temperature, ionic strength, etc. to accommodate factors such as probe length and the like. 
     The term “purified” as applied to nucleic acids or polypeptides generally denotes a nucleic acid or polypeptide that is essentially free from other components as determined by analytical techniques well known in the art (e.g., a purified polypeptide or polynucleotide forms a discrete band in an electrophoretic gel, chromatographic eluate, and/or a media subjected to density gradient centrifugation). For example, a nucleic acid or polypeptide that gives rise to essentially one band in an electrophoretic gel is “purified.” A purified nucleic acid or polypeptide is at least about 50% pure, usually at least about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 9′7%, about 98%, about 99%, about 99.5%, about 99.6%, about 99.′7%, about 99.8% or more pure (e.g., percent by weight on a molar basis). In a related sense, the invention provides methods of enriching compositions for one or more molecules of the invention, such as one or more polypeptides or polynucleotides of the invention. A composition is enriched for a molecule when there is a substantial increase in the concentration of the molecule after application of a purification or enrichment technique. A substantially pure polypeptide or polynucleotide of the invention (e.g., substantially pure metalloprotease polypeptide or polynucleotide encoding a metalloprotease polypeptide of the invention, respectively) will typically comprise at least about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98, about 99%, about 99.5% or more by weight (on a molar basis) of all macromolecular species in a particular composition. 
     The term “enriched” refers to a compound, polypeptide, cell, nucleic acid, amino acid, or other specified material or component that is present in a composition at a relative or absolute concentration that is higher than a starting composition. 
     In a related sense, the invention provides methods of enriching compositions for one or more molecules of the invention, such as one or more polypeptides of the invention (e.g., one or more metalloprotease polypeptides of the invention) or one or more nucleic acids of the invention (e.g., one or more nucleic acids encoding one or more metalloprotease polypeptides of the invention). A composition is enriched for a molecule when there is a substantial increase in the concentration of the molecule after application of a purification or enrichment technique. A substantially pure polypeptide or polynucleotide will typically comprise at least about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98, about 99%, about 99.5% or more by weight (on a molar basis) of all macromolecular species in a particular composition. 
     As used herein, the term “functional assay” refers to an assay that provides an indication of a protein&#39;s activity. In some embodiments, the term refers to assay systems in which a protein is analyzed for its ability to function in its usual capacity. For example, in the case of a protease, a functional assay involves determining the effectiveness of the protease to hydrolyze a proteinaceous substrate. 
     The terms “modified nucleic acid sequence” and “modified gene” are used interchangeably herein to refer to a nucleic acid sequence that includes a deletion, insertion or interruption of naturally occurring (i.e., wild-type) nucleic acid sequence. In some embodiments, the expression product of the modified nucleic acid sequence is a truncated protein (e.g., if the modification is a deletion or interruption of the sequence). In some embodiments, the truncated protein retains biological activity. In alternative embodiments, the expression product of the modified nucleic acid sequence is an elongated protein (e.g., modifications comprising an insertion into the nucleic acid sequence). In some embodiments, a nucleotide insertion in the nucleic acid sequence leads to a truncated protein (e.g., when the insertion results in the formation of a stop codon). Thus, an insertion may result in either a truncated protein or an elongated protein as an expression product. 
     A “mutant” nucleic acid sequence typically refers to a nucleic acid sequence that has an alteration in at least one codon occurring in a host cell&#39;s wild-type sequence such that the expression product of the mutant nucleic acid sequence is a protein with an altered amino acid sequence relative to the wild-type protein. The expression product may have an altered functional capacity (e.g., enhanced enzymatic activity). 
     As used herein, the phrase “alteration in substrate specificity” refers to changes in the substrate specificity of an enzyme. In some embodiments, a change in substrate specificity is defined as a change in k cat  and/or K m  for a particular substrate, resulting from mutations of the enzyme or alteration of reaction conditions. The substrate specificity of an enzyme is determined by comparing the catalytic efficiencies it exhibits with different substrates. These determinations find particular use in assessing the efficiency of mutant enzymes, as it is generally desired to produce variant enzymes that exhibit greater ratios of k cat /K m  for substrates of interest. However, it is not intended that the present invention be limited to any particular substrate composition or substrate specificity. 
     As used herein, “surface property” is used in reference to electrostatic charge, as well as properties such as the hydrophobicity and hydrophilicity exhibited by the surface of a protein. As used herein, the term “net charge” is defined as the sum of all charges present in a molecule. “Net charge changes” are made to a parent protein molecule to provide a variant that has a net charge that differs from that of the parent molecule (i.e., the variant has a net charge that is not the same as that of the parent molecule). For example, substitution of a neutral amino acid with a negatively charged amino acid or a positively charged amino acid with a neutral amino acid results in net charge of −1 with respect to the parent molecule. Substitution of a positively charged amino acid with a negatively charged amino acid results in a net charge of −2 with respect to the parent. Substitution of a neutral amino acid with a positively charged amino acid or a negatively charged amino acid with a neutral amino acid results in net charge of +1 with respect to the parent. Substitution of a negatively charged amino acid with a positively charged amino acid results in a net charge of +2 with respect to the parent. The net charge of a parent protein can also be altered by deletion and/or insertion of charged amino acids. A net change change applies to changes in charge of a variant versus a parent when measured at the same pH conditions. 
     The terms “thermally stable” and “thermostable” and “thermostability” refer to proteases that retain a specified amount of enzymatic activity after exposure to identified temperatures over a given period of time under conditions prevailing during the proteolytic, hydrolyzing, cleaning or other process of the invention, while being exposed to altered temperatures. “Altered temperatures” encompass increased or decreased temperatures. In some embodiments, the proteases retain at least about 50%, about 60%, about 70%, about 75%, about 80%, about 85%, about 90%, about 92%, about 95%, about 96%, about 97%, about 98%, or about 99% proteolytic activity after exposure to altered temperatures over a given time period, for example, at least about 60 minutes, about 120 minutes, about 180 minutes, about 240 minutes, about 300 minutes, etc. 
     The term “enhanced stability” in the context of an oxidation, chelator, thermal, chemical, autolytic and/or pH stable protease refers to a higher retained proteolytic activity over time as compared to other proteases (e.g., thermolysin proteases) and/or wild-type enzymes. 
     The term “diminished stability” in the context of an oxidation, chelator, thermal and/or pH stable protease refers to a lower retained proteolytic activity over time as compared to other proteases (e.g., thermolysin proteases) and/or wild-type enzymes. 
     The term “cleaning activity” refers to a cleaning performance achieved by a metalloprotease polypeptide or reference protease under conditions prevailing during the proteolytic, hydrolyzing, cleaning, or other process of the invention. In some embodiments, cleaning performance of a metalloprotease polypeptide or reference protease may be determined by using various assays for cleaning one or more various enzyme sensitive stains on an item or surface (e.g., a stain resulting from food, grass, blood, ink, milk, oil, and/or egg protein). Cleaning performance of a variant or reference protease can be determined by subjecting the stain on the item or surface to standard wash condition(s) and assessing the degree to which the stain is removed by using various chromatographic, spectrophotometric, or other quantitative methodologies. Exemplary cleaning assays and methods are known in the art and include, but are not limited to those described in WO 99/34011 and U.S. Pat. No. 6,605,458, both of which are herein incorporated by reference, as well as those cleaning assays and methods included in the Examples provided below. 
     The term “cleaning effective amount” of a metalloprotease polypeptide or reference protease refers to the amount of protease that achieves a desired level of enzymatic activity in a specific cleaning composition. Such effective amounts are readily ascertained by one of ordinary skill in the art and are based on many factors, such as the particular protease used, the cleaning application, the specific composition of the cleaning composition, and whether a liquid or dry (e.g., granular, tablet, bar) composition is required, etc. 
     The term “cleaning adjunct material” refers to any liquid, solid, or gaseous material included in cleaning composition other than a metalloprotease polypeptide of the invention. In some embodiments, the cleaning compositions of the present invention include one or more cleaning adjunct materials. Each cleaning adjunct material is typically selected depending on the particular type and form of cleaning composition (e.g., liquid, granule, powder, bar, paste, spray, tablet, gel, foam, or other composition). Preferably, each cleaning adjunct material is compatible with the protease enzyme used in the composition. 
     The term “enhanced performance” in the context of cleaning activity refers to an increased or greater cleaning activity by an enzyme with respect to a parent or reference protein as measured on certain enzyme sensitive stains such as egg, milk, grass, ink, oil, and/or blood, as determined by usual evaluation after a standard wash cycle and/or multiple wash cycles. 
     The term “diminished performance” in the context of cleaning activity refers to a decreased or lesser cleaning activity by an enzyme on certain enzyme sensitive stains such as egg, milk, grass or blood, as determined by usual evaluation after a standard wash cycle and/or multiple wash cycles. 
     Cleaning compositions and cleaning formulations include any composition that is suited for cleaning, bleaching, disinfecting, and/or sterilizing any object, item, and/or surface. Such compositions and formulations include, but are not limited to for example, liquid and/or solid compositions, including cleaning or detergent compositions (e.g., liquid, tablet, gel, bar, granule, and/or solid laundry cleaning or detergent compositions and fine fabric detergent compositions; hard surface cleaning compositions and formulations, such as for glass, wood, ceramic and metal counter tops and windows; carpet cleaners; oven cleaners; fabric fresheners; fabric softeners; and textile, laundry booster cleaning or detergent compositions, laundry additive cleaning compositions, and laundry pre-spotter cleaning compositions; dishwashing compositions, including hand or manual dishwash compositions (e.g., “hand” or “manual” dishwashing detergents) and automatic dishwashing compositions (e.g., “automatic dishwashing detergents”). 
     Cleaning composition or cleaning formulations, as used herein, include, unless otherwise indicated, granular or powder-form all-purpose or heavy-duty washing agents, especially cleaning detergents; liquid, granular, gel, solid, tablet, or paste-form all-purpose washing agents, especially the so-called heavy-duty liquid (HDL) detergent or heavy-duty powder detergent (HDD) types; liquid fine-fabric detergents; hand or manual dishwashing agents, including those of the high-foaming type; hand or manual dishwashing, automatic dishwashing, or dishware or tableware washing agents, including the various tablet, powder, solid, granular, liquid, gel, and rinse-aid types for household and institutional use; liquid cleaning and disinfecting agents, including antibacterial hand-wash types, cleaning bars, mouthwashes, denture cleaners, car shampoos, carpet shampoos, bathroom cleaners; hair shampoos and/or hair-rinses for humans and other animals; shower gels and foam baths and metal cleaners; as well as cleaning auxiliaries, such as bleach additives and “stain-stick” or pre-treat types. In some embodiments, granular compositions are in “compact” form; in some embodiments, liquid compositions are in a “concentrated” form. 
     As used herein, “fabric cleaning compositions” include hand and machine laundry detergent compositions including laundry additive compositions and compositions suitable for use in the soaking and/or pretreatment of stained fabrics (e.g., clothes, linens, and other textile materials). 
     As used herein, “non-fabric cleaning compositions” include non-textile (i.e., non-fabric) surface cleaning compositions, including, but not limited to for example, hand or manual or automatic dishwashing detergent compositions, oral cleaning compositions, denture cleaning compositions, and personal cleansing compositions. 
     As used herein, the term “fabric and/or hard surface cleaning and/or treatment composition” is a subset of cleaning and treatment compositions that includes, unless otherwise indicated, granular or powder-form all-purpose or “heavy-duty” washing agents, especially cleaning detergents; liquid, gel or paste-form all-purpose washing agents, especially the so-called heavy-duty liquid types; liquid fine-fabric detergents; hand dishwashing agents or light duty dishwashing agents, especially those of the high-foaming type; machine dishwashing agents, including the various tablet, granular, liquid and rinse-aid types for household and institutional use; liquid cleaning and disinfecting agents, car or carpet shampoos, bathroom cleaners including toilet bowl cleaners; fabric conditioning products including softening and/or freshening that may be in liquid, solid and/or dryer sheet form; as well as cleaning auxiliaries such as bleach additives and “stain-stick” or pre-treat types, substrate-laden products such as dryer added sheets. All of such products which are applicable may be in standard, concentrated or even highly concentrated form even to the extent that such products may in certain aspect be non-aqueous. 
     As used herein, the term “detergent composition” or “detergent formulation” is used in reference to a composition intended for use in a wash medium for the cleaning of soiled or dirty objects, including particular fabric and/or non-fabric objects or items. Such compositions of the present invention are not limited to any particular detergent composition or formulation. Indeed, in some embodiments, the detergents of the invention comprise at least one metalloprotease polypeptide of the invention and, in addition, one or more surfactants, transferase(s), hydrolytic enzymes, oxido reductases, builders (e.g., a builder salt), bleaching agents, bleach activators, bluing agents, fluorescent dyes, caking inhibitors, masking agents, enzyme activators, antioxidants, and/or solubilizers. In some instances, a builder salt is a mixture of a silicate salt and a phosphate salt, preferably with more silicate (e.g., sodium metasilicate) than phosphate (e.g., sodium tripolyphosphate). Some compositions of the invention, such as, but not limited to, cleaning compositions or detergent compositions, do not contain any phosphate (e.g., phosphate salt or phosphate builder). 
     As used herein, the term “bleaching” refers to the treatment of a material (e.g., fabric, laundry, pulp, etc.) or surface for a sufficient length of time and/or under appropriate pH and/or temperature conditions to effect a brightening (i.e., whitening) and/or cleaning of the material. Examples of chemicals suitable for bleaching include, but are not limited to, for example, ClO 2 , H 2 O 2 , peracids, NO 2 , etc. 
     As used herein, “wash performance” of a protease (e.g., a metalloprotease polypeptide of the invention) refers to the contribution of a metalloprotease polypeptide to washing that provides additional cleaning performance to the detergent as compared to the detergent without the addition of the metalloprotease polypeptide to the composition. Wash performance is compared under relevant washing conditions. In some test systems, other relevant factors, such as detergent composition, sud concentration, water hardness, washing mechanics, time, pH, and/or temperature, can be controlled in such a way that condition(s) typical for household application in a certain market segment (e.g., hand or manual dishwashing, automatic dishwashing, dishware cleaning, tableware cleaning, fabric cleaning, etc.) are imitated. 
     The term “relevant washing conditions” is used herein to indicate the conditions, particularly washing temperature, time, washing mechanics, sud concentration, type of detergent and water hardness, actually used in households in a hand dishwashing, automatic dishwashing, or laundry detergent market segment. 
     The term “improved wash performance” is used to indicate that a better end result is obtained in stain removal under relevant washing conditions, or that less metalloprotease polypeptide, on weight basis, is needed to obtain the same end result relative to the corresponding wild-type or starting parent protease. 
     As used herein, the term “disinfecting” refers to the removal of contaminants from the surfaces, as well as the inhibition or killing of microbes on the surfaces of items. It is not intended that the present invention be limited to any particular surface, item, or contaminant(s) or microbes to be removed. 
     The “compact” form of the cleaning compositions herein is best reflected by density and, in terms of composition, by the amount of inorganic filler salt. Inorganic filler salts are conventional ingredients of detergent compositions in powder form. In conventional detergent compositions, the filler salts are present in substantial amounts, typically about 17 to about 35% by weight of the total composition. In contrast, in compact compositions, the filler salt is present in amounts not exceeding about 15% of the total composition. In some embodiments, the filler salt is present in amounts that do not exceed about 10%, or more preferably, about 5%, by weight of the composition. In some embodiments, the inorganic filler salts are selected from the alkali and alkaline-earth-metal salts of sulfates and chlorides. In some embodiments, the filler salt is sodium sulfate. 
     As used herein in connection with a numerical value, the term “about” refers to a range of +/−0.5 of the numerical value, unless the term is otherwise specifically defined in context. For instance, the phrase a “pH value of about 6” refers to pH values of from 5.5 to 6.5, unless the pH value is specifically defined otherwise. 
     The position of an amino acid residue in a given amino acid sequence is typically numbered herein using the numbering of the position of the corresponding amino acid residue of the wild type  Paenibacillus  metalloprotease amino acid sequences shown in SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38. The  Paenibacillus  sp. metalloprotease amino acid sequences, thus serves as a reference parent sequence. A given amino acid sequence, such as a metalloprotease enzyme amino acid sequence and variants thereof described herein, can be aligned with the wild type metalloprotease sequence (e.g., SEQ ID NO: 3) using an alignment algorithm as described herein, and an amino acid residue in the given amino acid sequence that aligns (preferably optimally aligns) with an amino acid residue in the wild type sequence can be conveniently numbered by reference to the corresponding amino acid residue in the metalloprotease sequence. 
     Oligonucleotide synthesis and purification steps are typically performed according to specifications. Techniques and procedures are generally performed according to conventional methods well known in the art and various general references that are provided throughout this document. Procedures therein are believed to be well known to those of ordinary skill in the art and are provided for the convenience of the reader. 
     Metalloprotease Polypeptides of the Present Invention 
     The present invention provides novel metalloprotease enzyme polypeptides, which may be collectively referred to as “enzymes of the invention” or “polypeptides of the invention.” Polypeptides of the invention include isolated, recombinant, substantially pure, or non-naturally occurring polypeptides. In some embodiments, polypeptides of the invention are useful in cleaning applications and can be incorporated into cleaning compositions that are useful in methods of cleaning an item or a surface in need of cleaning. 
     In some embodiments, the enzyme of the present invention has 50, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38. In various embodiments, the enzyme of the present invention has 50, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to a metalloprotease enzyme from any genus in Tables 1.2, 2.2, 3.2, 4.2, 5.2, 6.2, 7.2 or 8.2. 
     In some embodiments, the enzyme of the present invention, including all embodiments supra, can be derived from a member of the order Bacillales or family Bacillaceae, Paenibacillaceae, Alicyclobacillaceae, or Lactobacillaceae. In some embodiments, the enzyme of the present invention, including all embodiments supra, can be derived from a  Bacillus, Alicyclobacillus, Geobacillus, Exiguobacterium, Lactobacillus , or  Paenibacillus  species. In some embodiments, the enzyme of the present invention, including all embodiments supra, can be derived from a member of the Pseudococcidae family. In some embodiments, the enzyme of the present invention, including all embodiments supra, can be derived from a  Planococcus  species. Various enzyme metalloproteases have been found that have a high identity to each other and to the  Paenibacillus  enzymes as shown in SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38. 
     In a particular embodiment, the invention is an enzyme derived from the genus  Paenibacillus . In a particular embodiment, the invention is an enzyme derived from the genus  Paenibacillus  and from the species  Paenibacillus  sp.,  Paenibacillus ehimensis, Paenibacillus hunanensis, Paenibacillus barcinonensis, Paenibacillus amylolyticus, Paenibacillus humicus  and  Paenibacillus polymyxa.    
     Described are compositions and methods relating to enzymes cloned from  Paenibacillus . The compositions and methods are based, in part, on the observation that cloned and expressed enzymes of the present invention have proteolytic activity in the presence of a detergent composition. Enzymes of the present invention also demonstrate excellent stability in detergent compositions. These features makes enzymes of the present invention well suited for use in a variety of cleaning applications, where the enzyme can hydrolyze proteins in the presence of surfactants and other components found in detergent compositions. 
     In some embodiments, the invention includes an isolated, recombinant, substantially pure, or non-naturally occurring enzyme having protease activity, which polypeptide comprises a polypeptide sequence having at least about 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence identity to a parent enzyme as provided herein. 
     In some embodiments, the polypeptide of the present invention, is a polypeptide having a specified degree of amino acid sequence homology to the exemplified polypeptides, e.g., at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or even at least 99% sequence homology to the amino acid sequences of SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38. Homology can be determined by amino acid sequence alignment, e.g., using a program such as BLAST, ALIGN, or CLUSTAL, as described herein. 
     Also provided are polypeptide enzymes of the present invention, having protease activity, said enzymes comprising an amino acid sequence which differs from the amino acid sequence of SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38 by no more than 50, no more than 40, no more than 30, no more than 35, no more than 25, no more than 20, no more than 19, no more than 18, no more than 17, no more than 16, no more than 15, no more than 14, no more than 13, no more than 12, no more than 11, no more than 10, no more than 9, no more than 8, no more than 7, no more than 6, no more than 5, no more than 4, no more than 3, no more than 2, or no more than 1 amino acid residue(s), when aligned using any of the previously described alignment methods. 
     As noted above, the variant enzyme polypeptides of the invention have enzymatic activities (e.g., protease activities) and thus are useful in cleaning applications, including but not limited to, methods for cleaning dishware items, tableware items, fabrics, and items having hard surfaces (e.g., the hard surface of a table, table top, wall, furniture item, floor, ceiling, etc.). Exemplary cleaning compositions comprising one or more variant metalloprotease enzyme polypeptides of the invention are described infra. The enzymatic activity (e.g., protease enzyme activity) of an enzyme polypeptide of the invention can be determined readily using procedures well known to those of ordinary skill in the art. The Examples presented infra describe methods for evaluating the enzymatic activity and cleaning performance. The performance of polypeptide enzymes of the invention in removing stains (e.g., a protein stain such as blood/milk/ink or egg yolk), cleaning hard surfaces, or cleaning laundry, dishware or tableware item(s) can be readily determined using procedures well known in the art and/or by using procedures set forth in the Examples. 
     The metalloprotease polypeptides of the invention have protease activity such that they are useful in casein hydrolysis, collagen hydrolysis, elastin hydrolysis, keratin hydrolysis, soy protein hydrolysis or corn meal protein hydrolysis. Thus, the polypeptides of the invention find use in other applications such as pretreatments for food, feed, or protein degradation. 
     The polypeptides of the invention are also useful in pretreatment of animal feed products, such as soy protein, corn meal, and other protein rich components. Pretreatment of these animal feed products with a polypeptide of the invention may help in the breakdown of complex proteins into their hydrolysates which are easily digestible by animals. 
     In yet other embodiments, the disclosed metalloprotease polypeptides find use in hydrolysis of corn soy protein. The disclosed metalloprotease polypeptides may be used alone or in combination with other proteases, amylases or lipases to produce peptides and free amino acids from the corn or soy protein. In some embodiments, the recovered proteins, peptides or amino acids can be subsequently used in animal feed or human food products. 
     The polypeptides of the invention are also useful in treatment of wounds, particularly in wound debridement. Wound debridement is the removal of dead, damaged or infected tissue to improve the healing potential of the remaining healthy tissue. Debridement is an important part of the healing process for burns and other serious wounds. The wounds or burns may be treated with a composition comprising a polypeptide of the invention which would result in removal of unwanted damaged tissue and improving the healthy tissue. 
     The metalloprotease polypeptides of the present invention can have protease activity over a broad range of pH conditions. In some embodiments, the metalloprotease polypeptides have protease activity on azo-casein as a substrate, as demonstrated in Examples 3.1 to 3.8. In some embodiments, the metalloprotease polypeptides have protease activity at a pH of from about 3.0 to about 12.0. In some embodiments, the metalloprotease polypeptides have protease activity at a pH of from about 4.0 to about 10.5. In some embodiments, the metalloprotease polypeptides have at least 70% of maximal protease activity at a pH of from about 5.5 to about 9.0. In some embodiments, the metalloprotease polypeptides have at least 80% of maximal protease activity at a pH of from about 6.0 to about 8.5. In some embodiments, the metalloprotease polypeptides have maximal protease activity at a pH of about 7.5. 
     In some embodiments, the metalloprotease polypeptides of the present invention have protease activity at a temperature range of from about 10° C. to about 100° C. In some embodiments, the metalloprotease polypeptides of the present invention have protease activity at a temperature range of from about 20° C. to about 90° C. In some embodiments, the metalloprotease polypeptides have at least 70% of maximal protease activity at a temperature of from about 45° C. to about 60° C. In some embodiments, the metalloprotease polypeptides have maximal protease activity at a temperature of 50° C. 
     In some embodiments, the metalloprotease polypeptides of the present invention demonstrate cleaning performance in a cleaning composition. Cleaning compositions often include ingredients harmful to the stability and performance of enzymes, making cleaning compositions a harsh environment for enzymes, e.g. metalloproteases, to retain function. Thus, it is not trivial for an enzyme to be put in a cleaning composition and expect enzymatic function (e.g. metalloprotease activity, such as demonstrated by cleaning performance). In some embodiments, the metalloprotease polypeptides of the present invention demonstrate cleaning performance in automatic dishwashing (ADW) detergent compositions. In some embodiments, the cleaning performance in automatic dishwashing (ADW) detergent compositions includes cleaning of egg yolk stains. In some embodiments, the metalloprotease polypeptides of the present invention demonstrate cleaning performance in laundry detergent compositions. In some embodiments, the cleaning performance in laundry detergent compositions includes cleaning of blood/milk/ink stains. In each of the cleaning compositions, the metalloprotease polypeptides of the present invention demonstrate cleaning performance with or without a bleach component. 
     The metalloprotease polypeptides of the invention have protease activity such that they are useful in casein hydrolysis, collagen hydrolysis, elastin hydrolysis, keratin hydrolysis, soy protein hydrolysis or corn meal protein hydrolysis. Thus, the polypeptides of the invention find use in other applications such as pretreatments for food, feed, or protein degradation. 
     A polypeptide of the invention can be subject to various changes, such as one or more amino acid insertions, deletions, and/or substitutions, either conservative or non-conservative, including where such changes do not substantially alter the enzymatic activity of the polypeptide. Similarly, a nucleic acid of the invention can also be subject to various changes, such as one or more substitutions of one or more nucleotides in one or more codons such that a particular codon encodes the same or a different amino acid, resulting in either a silent variation (e.g., when the encoded amino acid is not altered by the nucleotide mutation) or non-silent variation, one or more deletions of one or more nucleic acids (or codons) in the sequence, one or more additions or insertions of one or more nucleic acids (or codons) in the sequence, and/or cleavage of or one or more truncations of one or more nucleic acids (or codons) in the sequence. Many such changes in the nucleic acid sequence may not substantially alter the enzymatic activity of the resulting encoded polypeptide enzyme compared to the polypeptide enzyme encoded by the original nucleic acid sequence. A nucleic acid sequence of the invention can also be modified to include one or more codons that provide for optimum expression in an expression system (e.g., bacterial expression system), while, if desired, said one or more codons still encode the same amino acid(s). 
     In some embodiments, the present invention provides a genus of enzyme polypeptides having the desired enzymatic activity (e.g., protease enzyme activity or cleaning performance activity) which comprise sequences having the amino acid substitutions described herein and also which comprise one or more additional amino acid substitutions, such as conservative and non-conservative substitutions, wherein the polypeptide exhibits, maintains, or approximately maintains the desired enzymatic activity (e.g., proteolytic activity, as reflected in the cleaning activity or performance of the polypeptide enzymes of SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 and 38). Amino acid substitutions in accordance with the invention may include, but are not limited to, one or more non-conservative substitutions and/or one or more conservative amino acid substitutions. A conservative amino acid residue substitution typically involves exchanging a member within one functional class of amino acid residues for a residue that belongs to the same functional class (conservative amino acid residues are considered functionally homologous or conserved in calculating percent functional homology). A conservative amino acid substitution typically involves the substitution of an amino acid in an amino acid sequence with a functionally similar amino acid. For example, alanine, glycine, serine, and threonine are functionally similar and thus may serve as conservative amino acid substitutions for one another. Aspartic acid and glutamic acid may serve as conservative substitutions for one another. 
     Asparagine and glutamine may serve as conservative substitutions for one another. Arginine, lysine, and histidine may serve as conservative substitutions for one another. Isoleucine, leucine, methionine, and valine may serve as conservative substitutions for one another. Phenylalanine, tyrosine, and tryptophan may serve as conservative substitutions for one another. Other conservative amino acid substitution groups can be envisioned. For example, amino acids can be grouped by similar function or chemical structure or composition (e.g., acidic, basic, aliphatic, aromatic, sulfur-containing). For instance, an aliphatic grouping may comprise: Glycine (G), Alanine (A), Valine (V), Leucine (L), Isoleucine (I). Other groups containing amino acids that are considered conservative substitutions for one another include: aromatic: Phenylalanine (F), Tyrosine (Y), Tryptophan (W); sulfur-containing: Methionine (M), Cysteine (C); Basic: Arginine (R), Lysine (K), Histidine (H); Acidic: Aspartic acid (D), Glutamic acid (E); non-polar uncharged residues, Cysteine (C), Methionine (M), and Proline (P); hydrophilic uncharged residues: Serine (S), Threonine (T), Asparagine (N), and Glutamine (Q). Additional groupings of amino acids are well-known to those of skill in the art and described in various standard textbooks. Listing of a polypeptide sequence herein, in conjunction with the above substitution groups, provides an express listing of all conservatively substituted polypeptide sequences. 
     More conservative substitutions exist within the amino acid residue classes described above, which also or alternatively can be suitable. Conservation groups for substitutions that are more conservative include: valine-leucine-isoleucine, phenylalanine-tyrosine, lysine-arginine, alanine-valine, and asparagine-glutamine. 
     Conservatively substituted variations of a polypeptide sequence of the invention (e.g., variant metalloproteases of the invention) include substitutions of a small percentage, sometimes less than 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, or 6% of the amino acids of the polypeptide sequence, or less than 5%, 4%, 3%, 2%, or 1%, or less than 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 amino acid substitution of the amino acids of the polypeptide sequence, with a conservatively selected amino acid of the same conservative substitution group. 
     As described elsewhere herein in greater detail and in the Examples provided herein, polypeptides of the invention may have cleaning abilities that may be compared to known proteases, including known metalloproteases. 
     Nucleic Acids of the Invention 
     The invention provides isolated, non-naturally occurring, or recombinant nucleic acids which may be collectively referred to as “nucleic acids of the invention” or “polynucleotides of the invention”, which encode polypeptides of the invention. Nucleic acids of the invention, including all described below, are useful in recombinant production (e.g., expression) of polypeptides of the invention, typically through expression of a plasmid expression vector comprising a sequence encoding the polypeptide of interest or fragment thereof. As discussed above, polypeptides include metalloprotease polypeptides having enzymatic activity (e.g., proteolytic activity) which are useful in cleaning applications and cleaning compositions for cleaning an item or a surface (e.g., surface of an item) in need of cleaning. 
     In some embodiments, the invention provides an isolated, recombinant, substantially pure, or non-naturally occurring nucleic acid comprising a nucleotide sequence encoding any polypeptide (including any fusion protein, etc.) of the invention described above in the section entitled “Polypeptides of the Invention” and elsewhere herein. The invention also provides an isolated, recombinant, substantially pure, or non-naturally-occurring nucleic acid comprising a nucleotide sequence encoding a combination of two or more of any polypeptides of the invention described above and elsewhere herein. In some embodiments, the nucleic acids of the present invention has 50, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to SEQ ID NO: 4, 9, 14, 19, 24, 29, 34 and 39. 
     The present invention provides nucleic acids encoding a metalloprotease polypeptide of the present invention, wherein the metalloprotease polypeptide is a mature form having proteolytic activity, wherein the amino acid positions of the variant are numbered by correspondence with the amino acid sequence of  Paenibacillus  metalloprotease polypeptides set forth as SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38. 
     Nucleic acids of the invention can be generated by using any suitable synthesis, manipulation, and/or isolation techniques, or combinations thereof. For example, a polynucleotide of the invention may be produced using standard nucleic acid synthesis techniques, such as solid-phase synthesis techniques that are well-known to those skilled in the art. In such techniques, fragments of up to 50 or more nucleotide bases are typically synthesized, then joined (e.g., by enzymatic or chemical ligation methods) to form essentially any desired continuous nucleic acid sequence. The synthesis of the nucleic acids of the invention can be also facilitated by any suitable method known in the art, including but not limited to chemical synthesis using the classical phosphoramidite method (See e.g., Beaucage et al. Tetrahedron Letters 22:1859-69 [1981]); or the method described by Matthes et al. (See, Matthes et al., EMBO J. 3:801-805 [1984], as is typically practiced in automated synthetic methods. Nucleic acids of the invention also can be produced by using an automatic DNA synthesizer. Customized nucleic acids can be ordered from a variety of commercial sources (e.g., The Midland Certified Reagent Company, the Great American Gene Company, Operon Technologies Inc., and DNA2.0). Other techniques for synthesizing nucleic acids and related principles are known in the art (See e.g., Itakura et al., Ann. Rev. Biochem. 53:323 [1984]; and Itakura et al., Science 198:1056 [1984]). 
     As indicated above, recombinant DNA techniques useful in modification of nucleic acids are well known in the art. For example, techniques such as restriction endonuclease digestion, ligation, reverse transcription and cDNA production, and polymerase chain reaction (e.g., PCR) are known and readily employed by those of skill in the art. Nucleotides of the invention may also be obtained by screening cDNA libraries using one or more oligonucleotide probes that can hybridize to or PCR-amplify polynucleotides which encode a metalloprotease polypeptide polypeptide(s) of the invention. Procedures for screening and isolating cDNA clones and PCR amplification procedures are well known to those of skill in the art and described in standard references known to those skilled in the art. Some nucleic acids of the invention can be obtained by altering a naturally occurring polynucleotide backbone (e.g., that encodes an enzyme or parent protease) by, for example, a known mutagenesis procedure (e.g., site-directed mutagenesis, site saturation mutagenesis, and in vitro recombination). 
     Methods for Making Modified Metalloprotease Polypeptides of the Invention 
     A variety of methods are known in the art that are suitable for generating modified polynucleotides of the invention that encode metalloprotease polypeptides of the invention, including, but not limited to, for example, site-saturation mutagenesis, scanning mutagenesis, insertional mutagenesis, deletion mutagenesis, random mutagenesis, site-directed mutagenesis, and directed-evolution, as well as various other recombinatorial approaches. Methods for making modified polynucleotides and proteins (e.g., metalloprotease polypeptides) include DNA shuffling methodologies, methods based on non-homologous recombination of genes, such as ITCHY (See, Ostermeier et al., 7:2139-44 [1999]), SCRACHY (See, Lutz et al. 98:11248-53 [2001]), SHIPREC (See, Sieber et al., 19:456-60 [2001]), and NRR (See, Bittker et al., 20:1024-9 [2001]; Bittker et al., 101:7011-6 [2004]), and methods that rely on the use of oligonucleotides to insert random and targeted mutations, deletions and/or insertions (See, Ness et al., 20:1251-5 [2002]; Coco et al., 20:1246-50 [2002]; Zha et al., 4:34-9 [2003]; Glaser et al., 149:3903-13 [1992]). 
     Vectors, Cells, and Methods for Producing Metalloprotease Polypeptides of the Invention 
     The present invention provides vectors comprising at least one metalloprotease polynucleotide of the invention described herein (e.g., a polynucleotide encoding a metalloprotease polypeptide of the invention described herein), expression vectors or expression cassettes comprising at least one nucleic acid or polynucleotide of the invention, isolated, substantially pure, or recombinant DNA constructs comprising at least one nucleic acid or polynucleotide of the invention, isolated or recombinant cells comprising at least one polynucleotide of the invention, and compositions comprising one or more such vectors, nucleic acids, expression vectors, expression cassettes, DNA constructs, cells, cell cultures, or any combination or mixtures thereof. 
     In some embodiments, the invention provides recombinant cells comprising at least one vector (e.g., expression vector or DNA construct) of the invention which comprises at least one nucleic acid or polynucleotide of the invention. Some such recombinant cells are transformed or transfected with such at least one vector. Such cells are typically referred to as host cells. Some such cells comprise bacterial cells, including, but are not limited to  Bacillus  sp. cells, such as  B. subtilis  cells. The invention also provides recombinant cells (e.g., recombinant host cells) comprising at least one metalloprotease polypeptide of the invention. 
     In some embodiments, the invention provides a vector comprising a nucleic acid or polynucleotide of the invention. In some embodiments, the vector is an expression vector or expression cassette in which a polynucleotide sequence of the invention which encodes a metalloprotease polypeptide of the invention is operably linked to one or additional nucleic acid segments required for efficient gene expression (e.g., a promoter operably linked to the polynucleotide of the invention which encodes a metalloprotease polypeptide of the invention). A vector may include a transcription terminator and/or a selection gene, such as an antibiotic resistance gene, that enables continuous cultural maintenance of plasmid-infected host cells by growth in antimicrobial-containing media. 
     An expression vector may be derived from plasmid or viral DNA, or in alternative embodiments, contains elements of both. Exemplary vectors include, but are not limited to pC194, pJH101, pE194, pHP13 (See, Harwood and Cutting [eds.], Chapter 3, Molecular Biological Methods for  Bacillus , John Wiley &amp; Sons [1990]; suitable replicating plasmids for  B. subtilis  include those listed on p. 92) See also, Perego, Integrational Vectors for Genetic Manipulations in  Bacillus subtilis , in Sonenshein et al., [eds.]  Bacillus subtilis  and Other Gram-Positive Bacteria: Biochemistry, Physiology and Molecular Genetics, American Society for Microbiology, Washington, D.C. [1993], pp. 615-624), and p2JM103BBI. 
     For expression and production of a protein of interest (e.g., metalloprotease polypeptide) in a cell, at least one expression vector comprising at least one copy of a polynucleotide encoding the metalloprotease polypeptide, and in some instances comprising multiple copies, is transformed into the cell under conditions suitable for expression of the metalloprotease. In some embodiments of the present invention, a polynucleotide sequence encoding the metalloprotease polypeptide (as well as other sequences included in the vector) is integrated into the genome of the host cell, while in other embodiments, a plasmid vector comprising a polynucleotide sequence encoding the metalloprotease polypeptide remains as autonomous extra-chromosomal element within the cell. The invention provides both extrachromosomal nucleic acid elements as well as incoming nucleotide sequences that are integrated into the host cell genome. The vectors described herein are useful for production of the metalloprotease polypeptides of the invention. In some embodiments, a polynucleotide construct encoding the metalloprotease polypeptide is present on an integrating vector that enables the integration and optionally the amplification of the polynucleotide encoding the metalloprotease polypeptide into the host chromosome. Examples of sites for integration are well known to those skilled in the art. In some embodiments, transcription of a polynucleotide encoding a metalloprotease polypeptide of the invention is effectuated by a promoter that is the wild-type promoter for the selected precursor protease. In some other embodiments, the promoter is heterologous to the precursor protease, but is functional in the host cell. Specifically, examples of suitable promoters for use in bacterial host cells include, but are not limited to, for example, the amyE, amyQ, amyL, pstS, sacB, pSPAC, pAprE, pVeg, pHpaII promoters, the promoter of the  B. stearothermophilus  maltogenic amylase gene, the  B. amyloliquefaciens  (BAN) amylase gene, the  B. subtilis  alkaline protease gene, the  B. clausii  alkaline protease gene the  B. pumilis  xylosidase gene, the  B. thuringiensis  cryIIIA, and the  B. licheniformis  alpha-amylase gene. Additional promoters include, but are not limited to the A4 promoter, as well as phage Lambda PR or PL promoters, and the  E. coli  lac, trp or tac promoters. 
     Metalloprotease polypeptides of the present invention can be produced in host cells of any suitable microorganism, including bacteria and fungi. In some embodiments, metalloprotease polypeptides of the present invention can be produced in Gram-positive bacteria. In some embodiments, the host cells are  Bacillus  spp.,  Streptomyces  spp.,  Escherichia  spp.,  Aspergillus  spp.,  Trichoderma  spp.,  Pseudomonas  spp.,  Corynebacterium  spp.,  Saccharomyces  spp., or  Pichia  spp. In some embodiments, the metalloprotease polypeptides are produced by  Bacillus  sp. host cells. Examples of  Bacillus  sp. host cells that find use in the production of the metalloprotease polypeptides of the invention include, but are not limited to  B. licheniformis, B. lentus, B. subtilis, B. amyloliquefaciens, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. coagulans, B. circulans, B. pumilis, B. thuringiensis, B. clausii , and  B. megaterium , as well as other organisms within the genus  Bacillus . In some embodiments,  B. subtilis  host cells are used for production of metalloprotease polypeptides. U.S. Pat. Nos. 5,264,366 and 4,760,025 (RE 34,606) describe various  Bacillus  host strains that can be used for producing metalloprotease polypeptide of the invention, although other suitable strains can be used. 
     Several bacterial strains that can be used to produce metalloprotease polypeptides of the invention include non-recombinant (i.e., wild-type)  Bacillus  sp. strains, as well as variants of naturally-occurring strains and/or recombinant strains. In some embodiments, the host strain is a recombinant strain, wherein a polynucleotide encoding a polypeptide of interest has been introduced into the host. In some embodiments, the host strain is a  B. subtilis  host strain and particularly a recombinant  Bacillus subtilis  host strain. Numerous  B. subtilis  strains are known, including, but not limited to for example, 1A6 (ATCC 39085), 168 (1A01), SB19, W23, Ts85, B637, PB1753 through PB1758, PB3360, JH642, 1A243 (ATCC 39,087), ATCC 21332, ATCC 6051, MI113, DE100 (ATCC 39,094), GX4931, PBT 110, and PEP 211strain (See e.g., Hoch et al., Genetics 73:215-228 [1973]; See also, U.S. Pat. Nos. 4,450,235 and 4,302,544, and EP 0134048, each of which is incorporated by reference in its entirety). The use of  B. subtilis  as an expression host cells is well known in the art (See e.g., Palva et al., Gene 19:81-87 [1982]; Fahnestock and Fischer, J. Bacteriol., 165:796-804 [1986]; and Wang et al., Gene 69:39-47 [1988]). 
     In some embodiments, the  Bacillus  host cell is a  Bacillus  sp. that includes a mutation or deletion in at least one of the following genes, degU, degS, degR and degQ. In some embodiments, the mutation is in a degU gene, and in some embodiments the mutation is degU(Hy)32 (See e.g., Msadek et al., J. Bacteriol. 172:824-834 [1990]; and Olmos et al., Mol. Gen. Genet. 253:562-567 [1997]). In some embodiments, the  Bacillus  host comprises a mutation or deletion in scoC4 (See e.g., Caldwell et al., J. Bacteriol. 183:7329-7340 [2001]); spollE (See e.g., Arigoni et al., Mol. Microbiol. 31:1407-1415 [1999]); and/or oppA or other genes of the opp operon (See e.g., Perego et al., Mol. Microbiol. 5:173-185 [1991]). Indeed, it is contemplated that any mutation in the opp operon that causes the same phenotype as a mutation in the oppA gene will find use in some embodiments of the altered  Bacillus  strain of the invention. In some embodiments, these mutations occur alone, while in other embodiments, combinations of mutations are present. In some embodiments, an altered  Bacillus  host cell strain that can be used to produce a metalloprotease polypeptide of the invention is a  Bacillus  host strain that already includes a mutation in one or more of the above-mentioned genes. In addition,  Bacillus  sp. host cells that comprise mutation(s) and/or deletions of endogenous protease genes find use. In some embodiments, the  Bacillus  host cell comprises a deletion of the aprE and the nprE genes. In other embodiments, the  Bacillus  sp. host cell comprises a deletion of 5 protease genes, while in other embodiments, the  Bacillus  sp. host cell comprises a deletion of 9 protease genes (See e.g., U.S. Pat. Appln. Pub. No. 2005/0202535, incorporated herein by reference). 
     Host cells are transformed with at least one nucleic acid encoding at least one metalloprotease polypeptide of the invention using any suitable method known in the art. Methods for introducing a nucleic acid (e.g., DNA) into  Bacillus  cells or  E. coli  cells utilizing plasmid DNA constructs or vectors and transforming such plasmid DNA constructs or vectors into such cells are well known. In some embodiments, the plasmids are subsequently isolated from  E. coli  cells and transformed into  Bacillus  cells. However, it is not essential to use intervening microorganisms such as  E. coli , and in some embodiments, a DNA construct or vector is directly introduced into a  Bacillus  host. 
     Those of skill in the art are well aware of suitable methods for introducing nucleic acid sequences of the invention into  Bacillus  cells (See e.g., Ferrari et al., “Genetics,” in Harwood et al. [eds.],  Bacillus , Plenum Publishing Corp. [1989], pp. 57-72; Saunders et al., J. Bacteriol. 157:718-726 [1984]; Hoch et al., J. Bacteriol. 93:1925-1937 [1967]; Mann et al., Current Microbiol. 13:131-135 [1986]; Holubova, Folia Microbiol. 30:97 [1985]; Chang et al., Mol. Gen. Genet. 168:11-115 [1979]; Vorobjeva et al., FEMS Microbiol. Lett. 7:261-263 [1980]; Smith et al., Appl. Env. Microbiol. 51:634 [1986]; Fisher et al., Arch. Microbiol. 139:213-217 [1981]; and McDonald, J. Gen. Microbiol. 130:203 [1984]). Indeed, such methods as transformation, including protoplast transformation and transfection, transduction, and protoplast fusion are well known and suited for use in the present invention. Methods known in the art to transform  Bacillus  cells include such methods as plasmid marker rescue transformation, which involves the uptake of a donor plasmid by competent cells carrying a partially homologous resident plasmid (See, Contente et al., Plasmid 2:555-571 [1979]; Haima et al., Mol. Gen. Genet. 223:185-191 [1990]; Weinrauch et al., J. Bacteriol. 154:1077-1087 [1983]; and Weinrauch et al., J. Bacteriol. 169:1205-1211 [1987]). In this method, the incoming donor plasmid recombines with the homologous region of the resident “helper” plasmid in a process that mimics chromosomal transformation. 
     In addition to commonly used methods, in some embodiments, host cells are directly transformed with a DNA construct or vector comprising a nucleic acid encoding a metalloprotease polypeptide of the invention (i.e., an intermediate cell is not used to amplify, or otherwise process, the DNA construct or vector prior to introduction into the host cell). Introduction of the DNA construct or vector of the invention into the host cell includes those physical and chemical methods known in the art to introduce a nucleic acid sequence (e.g., DNA sequence) into a host cell without insertion into the host genome. Such methods include, but are not limited to calcium chloride precipitation, electroporation, naked DNA, liposomes and the like. In additional embodiments, DNA constructs or vector are co-transformed with a plasmid, without being inserted into the plasmid. In further embodiments, a selective marker is deleted from the altered  Bacillus  strain by methods known in the art (See, Stahl et al., J. Bacteriol. 158:411-418 [1984]; and Palmeros et al., Gene 247:255-264 [2000]). 
     In some embodiments, the transformed cells of the present invention are cultured in conventional nutrient media. The suitable specific culture conditions, such as temperature, pH and the like are known to those skilled in the art and are well described in the scientific literature. In some embodiments, the invention provides a culture (e.g., cell culture) comprising at least one metalloprotease polypeptide or at least one nucleic acid of the invention. 
     In some embodiments, host cells transformed with at least one polynucleotide sequence encoding at least one metalloprotease polypeptide of the invention are cultured in a suitable nutrient medium under conditions permitting the expression of the present protease, after which the resulting protease is recovered from the culture. In some embodiments, the protease produced by the cells is recovered from the culture medium by conventional procedures, including, but not limited to for example, separating the host cells from the medium by centrifugation or filtration, precipitating the proteinaceous components of the supernatant or filtrate by means of a salt (e.g., ammonium sulfate), chromatographic purification (e.g., ion exchange, gel filtration, affinity, etc.). 
     In some embodiments, a metalloprotease polypeptide produced by a recombinant host cell is secreted into the culture medium. A nucleic acid sequence that encodes a purification facilitating domain may be used to facilitate purification of proteins. A vector or DNA construct comprising a polynucleotide sequence encoding a metalloprotease polypeptide may further comprise a nucleic acid sequence encoding a purification facilitating domain to facilitate purification of the metalloprotease polypeptide (See e.g., Kroll et al., DNA Cell Biol. 12:441-53 [1993]). Such purification facilitating domains include, but are not limited to, for example, metal chelating peptides such as histidine-tryptophan modules that allow purification on immobilized metals (See, Porath, Protein Expr. Purif. 3:263-281 [1992]), protein A domains that allow purification on immobilized immunoglobulin, and the domain utilized in the FLAGS extension/affinity purification system. The inclusion of a cleavable linker sequence such as Factor XA or enterokinase (e.g., sequences available from Invitrogen, San Diego, Calif.) between the purification domain and the heterologous protein also find use to facilitate purification. 
     Assays for detecting and measuring the enzymatic activity of an enzyme, such as a metalloprotease polypeptide of the invention, are well known. Various assays for detecting and measuring activity of proteases (e.g., metalloprotease polypeptides of the invention), are also known to those of ordinary skill in the art. In particular, assays are available for measuring protease activity that are based on the release of acid-soluble peptides from casein or hemoglobin, measured as absorbance at 280 nm or colorimetrically using the Folin method. Other exemplary assays involve the solubilization of chromogenic substrates (See e.g., Ward, “Proteinases,” in Fogarty (ed.)., Microbial Enzymes and Biotechnology, Applied Science, London, [1983], pp. 251-317). Other exemplary assays include, but are not limited to succinyl-Ala-Ala-Pro-Phe-para nitroanilide assay (suc-AAPF-pNA)(SEQ ID NO: 43) and the 2,4,6-trinitrobenzene sulfonate sodium salt assay (TNBS assay). Numerous additional references known to those in the art provide suitable methods (See e.g., Wells et al., Nucleic Acids Res. 11:7911-7925 [1983]; Christianson et al., Anal. Biochem. 223:119-129 [1994]; and Hsia et al., Anal Biochem. 242:221-227 [1999]). 
     A variety of methods can be used to determine the level of production of a mature protease (e.g., mature metalloprotease polypeptides of the present invention) in a host cell. Such methods include, but are not limited to, for example, methods that utilize either polyclonal or monoclonal antibodies specific for the protease. Exemplary methods include, but are not limited to enzyme-linked immunosorbent assays (ELISA), radioimmunoassays (MA), fluorescent immunoassays (FIA), and fluorescent activated cell sorting (FACS). These and other assays are well known in the art (See e.g., Maddox et al., J. Exp. Med. 158:1211 [1983]). 
     In some other embodiments, the invention provides methods for making or producing a mature metalloprotease polypeptide of the invention. A mature metalloprotease polypeptide does not include a signal peptide or a propeptide sequence. Some methods comprise making or producing a metalloprotease polypeptide of the invention in a recombinant bacterial host cell, such as for example, a  Bacillus  sp. cell (e.g., a  B. subtilis  cell). In some embodiments, the invention provides a method of producing a metalloprotease polypeptide of the invention, the method comprising cultivating a recombinant host cell comprising a recombinant expression vector comprising a nucleic acid encoding a metalloprotease polypeptide of the invention under conditions conducive to the production of the metalloprotease polypeptide. Some such methods further comprise recovering the metalloprotease polypeptide from the culture. 
     In some embodiments the invention provides methods of producing a metalloprotease polypeptide of the invention, the methods comprising: (a) introducing a recombinant expression vector comprising a nucleic acid encoding a metalloprotease polypeptide of the invention into a population of cells (e.g., bacterial cells, such as  B. subtilis  cells); and (b) culturing the cells in a culture medium under conditions conducive to produce the metalloprotease polypeptide encoded by the expression vector. Some such methods further comprise: (c) isolating the metalloprotease polypeptide from the cells or from the culture medium. 
     Fabric and Home Care Products 
     In some embodiments, the metalloprotease polypeptides of the present invention can be used in compositions comprising an adjunct material and a metalloprotease polypeptide, wherein the composition is a fabric and home care product. 
     In some embodiments, the fabric and home care product compositions comprising at least one metalloprotease polypeptide comprise one or more of the following ingredients (based on total composition weight): from about 0.0005 wt % to about 0.1 wt %, from about 0.001 wt % to about 0.05 wt %, or even from about 0.002 wt % to about 0.03 wt % of said metalloprotease polypeptide; and one or more of the following: from about 0.00003 wt % to about 0.1 wt % fabric hueing agent; from about 0.001 wt % to about 5 wt %, perfume capsules; from about 0.001 wt % to about 1 wt %, cold-water soluble brighteners; from about 0.00003 wt % to about 0.1 wt % bleach catalysts; from about 0.00003 wt % to about 0.1 wt % first wash lipases; from about 0.00003 wt % to about 0.1 wt % bacterial cleaning cellulases; and/or from about 0.05 wt % to about 20 wt % Guerbet nonionic surfactants. 
     In some embodiments, the fabric and home care product composition is a liquid laundry detergent or a dishwashing detergent, such as an automatic dishwashing (ADW) detergent or hand dishwashing detergent. 
     It is intended that the fabric and home care product is provided in any suitable form, including a fluid or solid, or granular, powder, solid, bar, liquid, tablet, gel, or paste form. The fabric and home care product may be in the form of a unit dose pouch, especially when in the form of a liquid, and typically the fabric and home care product is at least partially, or even completely, enclosed by a water-soluble pouch. In addition, in some embodiments of the fabric and home care products comprising at least one metalloprotease polypeptide, the fabric and home care product may have any combination of parameters and/or characteristics detailed above. 
     Compositions Having the Metalloprotease Polypeptide of the Present Invention 
     Unless otherwise noted, all component or composition levels provided herein are made in reference to the active level of that component or composition, and are exclusive of impurities, for example, residual solvents or by-products, which may be present in commercially available sources. Enzyme components weights are based on total active protein. All percentages and ratios are calculated by weight unless otherwise indicated. All percentages and ratios are calculated based on the total composition unless otherwise indicated. Compositions of the invention include cleaning compositions, such as detergent compositions. In the exemplified detergent compositions, the enzymes levels are expressed by pure enzyme by weight of the total composition and unless otherwise specified, the detergent ingredients are expressed by weight of the total compositions. 
     As indicated herein, in some embodiments, the cleaning compositions of the present invention further comprise adjunct materials including, but not limited to, surfactants, builders, bleaches, bleach activators, bleach catalysts, other enzymes, enzyme stabilizing systems, chelants, optical brighteners, soil release polymers, dye transfer agents, dispersants, suds suppressors, dyes, perfumes, colorants, filler salts, hydrotropes, photoactivators, fluorescers, fabric conditioners, hydrolyzable surfactants, preservatives, anti-oxidants, anti-shrinkage agents, anti-wrinkle agents, germicides, fungicides, color speckles, silvercare, anti-tarnish and/or anti-corrosion agents, alkalinity sources, solubilizing agents, carriers, processing aids, pigments, and pH control agents (See e.g., U.S. Pat. Nos. 6,610,642, 6,605,458, 5,705,464, 5,710,115, 5,698,504, 5,695,679, 5,686,014 and 5,646,101, all of which are incorporated herein by reference). Embodiments of specific cleaning composition materials are exemplified in detail below. In embodiments in which the cleaning adjunct materials are not compatible with the metalloprotease polypeptides of the present invention in the cleaning compositions, then suitable methods of keeping the cleaning adjunct materials and the protease(s) separated (i.e., not in contact with each other) until combination of the two components is appropriate are used. Such separation methods include any suitable method known in the art (e.g., gelcaps, encapsulation, tablets, physical separation, etc.). 
     The cleaning compositions of the present invention are advantageously employed for example, in laundry applications, hard surface cleaning, dishwashing applications, including automatic dishwashing and hand dishwashing, as well as cosmetic applications such as dentures, teeth, hair and skin. In addition, due to the unique advantages of increased effectiveness in lower temperature solutions, the enzymes of the present invention are ideally suited for laundry applications. Furthermore, the enzymes of the present invention find use in granular and liquid compositions. 
     The metalloprotease polypeptides of the present invention also find use in cleaning additive products. In some embodiments, low temperature solution cleaning applications find use. In some embodiments, the present invention provides cleaning additive products including at least one enzyme of the present invention is ideally suited for inclusion in a wash process when additional bleaching effectiveness is desired. Such instances include, but are not limited to low temperature solution cleaning applications. In some embodiments, the additive product is in its simplest form, one or more proteases. In some embodiments, the additive is packaged in dosage form for addition to a cleaning process. In some embodiments, the additive is packaged in dosage form for addition to a cleaning process where a source of peroxygen is employed and increased bleaching effectiveness is desired. Any suitable single dosage unit form finds use with the present invention, including but not limited to pills, tablets, gelcaps, or other single dosage units such as pre-measured powders or liquids. In some embodiments, filler(s) or carrier material(s) are included to increase the volume of such compositions. Suitable filler or carrier materials include, but are not limited to, various salts of sulfate, carbonate and silicate as well as talc, clay and the like. Suitable filler or carrier materials for liquid compositions include, but are not limited to water or low molecular weight primary and secondary alcohols including polyols and diols. Examples of such alcohols include, but are not limited to, methanol, ethanol, propanol and isopropanol. In some embodiments, the compositions contain from about 5% to about 90% of such materials. Acidic fillers find use to reduce pH. Alternatively, in some embodiments, the cleaning additive includes adjunct ingredients, as more fully described below. 
     The present cleaning compositions and cleaning additives require an effective amount of at least one of the metalloprotease polypeptides provided herein, alone or in combination with other proteases and/or additional enzymes. The required level of enzyme is achieved by the addition of one or more metalloprotease polypeptides of the present invention. Typically the present cleaning compositions comprise at least about 0.0001 weight percent, from about 0.0001 to about 10, from about 0.001 to about 1, or from about 0.01 to about 0.1 weight percent of at least one of the metalloprotease polypeptides of the present invention. 
     The cleaning compositions herein are typically formulated such that, during use in aqueous cleaning operations, the wash water will have a pH of from about 4.0 to about 11.5, or even from about 5.0 to about 11.5, or even from about 5.0 to about 8.0, or even from about 7.5 to about 10.5. Liquid product formulations are typically formulated to have a pH from about 3.0 to about 9.0 or even from about 3 to about 5. Granular laundry products are typically formulated to have a pH from about 9 to about 11. Techniques for controlling pH at recommended usage levels include the use of buffers, alkalis, acids, etc., and are well known to those skilled in the art. 
     Suitable “low pH cleaning compositions” typically have a pH of from about 3 to about 5, and are typically free of surfactants that hydrolyze in such a pH environment. Such surfactants include sodium alkyl sulfate surfactants that comprise at least one ethylene oxide moiety or even from about 1 to about 16 moles of ethylene oxide. Such cleaning compositions typically comprise a sufficient amount of a pH modifier, such as sodium hydroxide, monoethanolamine or hydrochloric acid, to provide such cleaning composition with a pH of from about 3 to about 5. Such compositions typically comprise at least one acid stable enzyme. In some embodiments, the compositions are liquids, while in other embodiments, they are solids. The pH of such liquid compositions is typically measured as a neat pH. The pH of such solid compositions is measured as a 10% solids solution of said composition wherein the solvent is distilled water. In these embodiments, all pH measurements are taken at 20° C., unless otherwise indicated. 
     In some embodiments, when the metalloprotease polypeptide (s) is/are employed in a granular composition or liquid, it is desirable for the metalloprotease polypeptide to be in the form of an encapsulated particle to protect the metalloprotease polypeptide from other components of the granular composition during storage. In addition, encapsulation is also a means of controlling the availability of the metalloprotease polypeptide during the cleaning process. In some embodiments, encapsulation enhances the performance of the metalloprotease polypeptide (s) and/or additional enzymes. In this regard, the metalloprotease polypeptides of the present invention are encapsulated with any suitable encapsulating material known in the art. In some embodiments, the encapsulating material typically encapsulates at least part of the metalloprotease polypeptide (s) of the present invention. Typically, the encapsulating material is water-soluble and/or water-dispersible. In some embodiments, the encapsulating material has a glass transition temperature (Tg) of 0° C. or higher. Glass transition temperature is described in more detail in WO 97/11151. The encapsulating material is typically selected from consisting of carbohydrates, natural or synthetic gums, chitin, chitosan, cellulose and cellulose derivatives, silicates, phosphates, borates, polyvinyl alcohol, polyethylene glycol, paraffin waxes, and combinations thereof. When the encapsulating material is a carbohydrate, it is typically selected from monosaccharides, oligosaccharides, polysaccharides, and combinations thereof. In some typical embodiments, the encapsulating material is a starch (See e.g., EP 0 922 499; U.S. Pat. Nos. 4,977,252; 5,354,559, and 5,935,826). In some embodiments, the encapsulating material is a microsphere made from plastic such as thermoplastics, acrylonitrile, methacrylonitrile, polyacrylonitrile, polymethacrylonitrile and mixtures thereof commercially available microspheres that find use include, but are not limited to those supplied by EXPANCEL® (Stockviksverken, Sweden), and PM 6545, PM 6550, PM 7220, PM 7228, EXTENDOSPHERES®, LUXSIL®, Q-CEL®, and SPHERICEL® (PQ Corp., Valley Forge, Pa.). 
     As described herein, the metalloprotease polypeptides of the present invention find particular use in the cleaning industry, including, but not limited to laundry and dish detergents. These applications place enzymes under various environmental stresses. The metalloprotease polypeptides of the present invention provide advantages over many currently used enzymes, due to their stability under various conditions. 
     Indeed, there are a variety of wash conditions including varying detergent formulations, wash water volumes, wash water temperatures, and lengths of wash time, to which proteases involved in washing are exposed. In addition, detergent formulations used in different geographical areas have different concentrations of their relevant components present in the wash water. For example, European detergents typically have about 4500-5000 ppm of detergent components in the wash water, while Japanese detergents typically have approximately 667 ppm of detergent components in the wash water. In North America, particularly the United States, detergents typically have about 975 ppm of detergent components present in the wash water. 
     A low detergent concentration system includes detergents where less than about 800 ppm of the detergent components are present in the wash water. Japanese detergents are typically considered low detergent concentration system as they have approximately 667 ppm of detergent components present in the wash water. 
     A medium detergent concentration includes detergents where between about 800 ppm and about 2000 ppm of the detergent components are present in the wash water. North American detergents are generally considered to be medium detergent concentration systems as they have approximately 975 ppm of detergent components present in the wash water. Brazil typically has approximately 1500 ppm of detergent components present in the wash water. 
     A high detergent concentration system includes detergents where greater than about 2000 ppm of the detergent components are present in the wash water. European detergents are generally considered to be high detergent concentration systems as they have approximately 4500-5000 ppm of detergent components in the wash water. 
     Latin American detergents are generally high suds phosphate builder detergents and the range of detergents used in Latin America can fall in both the medium and high detergent concentrations as they range from 1500 ppm to 6000 ppm of detergent components in the wash water. As mentioned above, Brazil typically has approximately 1500 ppm of detergent components present in the wash water. However, other high suds phosphate builder detergent geographies, not limited to other Latin American countries, may have high detergent concentration systems up to about 6000 ppm of detergent components present in the wash water. 
     In light of the foregoing, it is evident that concentrations of detergent compositions in typical wash solutions throughout the world varies from less than about 800 ppm of detergent to about 6000 ppm in high suds phosphate builder geographies. 
     The concentrations of the typical wash solutions are determined empirically. For example, in the U.S., a typical washing machine holds a volume of about 64.4 L of wash solution. Accordingly, in order to obtain a concentration of about 975 ppm of detergent within the wash solution about 62.79 g of detergent composition must be added to the 64.4 L of wash solution. This amount is the typical amount measured into the wash water by the consumer using the measuring cup provided with the detergent. 
     As a further example, different geographies use different wash temperatures. The temperature of the wash water in Japan is typically less than that used in Europe. For example, the temperature of the wash water in North America and Japan is typically between about 10 and about 40° C. (e.g., about 20° C.), whereas the temperature of wash water in Europe is typically between about 30 and about 60° C. (e.g., about 40° C.). However, in the interest of saving energy, many consumers are switching to using cold water washing. In addition, in some further regions, cold water is typically used for laundry, as well as dish washing applications. In some embodiments, the “cold water washing” of the present invention utilizes “cold water detergent” suitable for washing at temperatures from about 10° C. to about 40° C., or from about 20° C. to about 30° C., or from about 15° C. to about 25° C., as well as all other combinations within the range of about 15° C. to about 35° C., and all ranges within 10° C. to 40° C. 
     As a further example, different geographies typically have different water hardness. Water hardness is usually described in terms of the grains per gallon mixed Ca 2+ /Mg 2+ . Hardness is a measure of the amount of calcium (Ca 2+ ) and magnesium (Mg 2+ ) in the water. Most water in the United States is hard, but the degree of hardness varies. Moderately hard (60-120 ppm) to hard (121-181 ppm) water has 60 to 181 parts per million (parts per million converted to grains per U.S. gallon is ppm # divided by 17.1 equals grains per gallon) of hardness minerals. 
     
       
         
           
               
               
               
             
               
                   
               
               
                 Water 
                 Grains per gallon 
                 Parts per million 
               
               
                   
               
             
            
               
                 Soft 
                 less than 1.0 
                 less than 17 
               
               
                 Slightly hard 
                 1.0 to 3.5 
                 17 to 60 
               
               
                 Moderately hard 
                 3.5 to 7.0 
                 60 to 120 
               
               
                 Hard 
                 7.0 to 10.5 
                 120 to 180 
               
               
                 Very hard 
                 greater than 10.5 
                 greater than 180 
               
               
                   
               
            
           
         
       
     
     European water hardness is typically greater than about 10.5 (for example about 10.5 to about 20.0) grains per gallon mixed Ca 2+ /Mg 2+  (e.g., about 15 grains per gallon mixed Ca 2+ /Mg 2+ ). North American water hardness is typically greater than Japanese water hardness, but less than European water hardness. For example, North American water hardness can be between about 3 to about 10 grains, about 3 to about 8 grains or about 6 grains. Japanese water hardness is typically lower than North American water hardness, usually less than about 4, for example about 3 grains per gallon mixed Ca 2+ /Mg 2+ . 
     Accordingly, in some embodiments, the present invention provides metalloprotease polypeptides that show surprising wash performance in at least one set of wash conditions (e.g., water temperature, water hardness, and/or detergent concentration). In some embodiments, the metalloprotease polypeptides of the present invention are comparable in wash performance to other metalloprotease polypeptide proteases. In some embodiments of the present invention, the metalloprotease polypeptides provided herein exhibit enhanced oxidative stability, enhanced thermal stability, enhanced cleaning capabilities under various conditions, and/or enhanced chelator stability. In addition, the metalloprotease polypeptides of the present invention find use in cleaning compositions that do not include detergents, again either alone or in combination with builders and stabilizers. 
     In some embodiments of the present invention, the cleaning compositions comprise at least one metalloprotease polypeptide of the present invention at a level from about 0.00001% to about 10% by weight of the composition and the balance (e.g., about 99.999% to about 90.0%) comprising cleaning adjunct materials by weight of composition. In some other embodiments of the present invention, the cleaning compositions of the present invention comprises at least one metalloprotease polypeptide at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% by weight of the composition and the balance of the cleaning composition (e.g., about 99.9999% to about 90.0%, about 99.999% to about 98%, about 99.995% to about 99.5% by weight) comprising cleaning adjunct materials. 
     In some embodiments, the cleaning compositions of the present invention comprise one or more additional detergent enzymes, which provide cleaning performance and/or fabric care and/or dishwashing benefits. Examples of suitable enzymes include, but are not limited to, acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, or any combinations or mixtures thereof. In some embodiments, a combination of enzymes is used (i.e., a “cocktail”) comprising conventional applicable enzymes like protease, lipase, cutinase and/or cellulase in conjunction with amylase is used. 
     In addition to the metalloprotease polypeptides provided herein, any other suitable protease finds use in the compositions of the present invention. Suitable proteases include those of animal, vegetable or microbial origin. In some embodiments, microbial proteases are used. In some embodiments, chemically or genetically modified mutants are included. In some embodiments, the protease is a serine protease, preferably an alkaline microbial protease or a trypsin-like protease. Examples of alkaline proteases include subtilisins, especially those derived from  Bacillus  (e.g., subtilisin,  lentus, amyloliquefaciens , subtilisin Carlsberg, subtilisin 309, subtilisin 147 and subtilisin 168). Additional examples include those mutant proteases described in U.S. Pat. Nos. RE 34,606, 5,955,340, 5,700,676, 6,312,936, and 6,482,628, all of which are incorporated herein by reference. Additional protease examples include, but are not limited to trypsin (e.g., of porcine or bovine origin), and the  Fusarium  protease described in WO 89/06270. In some embodiments, commercially available protease enzymes that find use in the present invention include, but are not limited to MAXATASE®, MAXACAL™ MAXAPEM™, OPTICLEAN®, OPTIMASE®, PROPERASE®, PURAFECT®, PURAFECT® OXP, PURAMAX™, EXCELLASE™, and PURAFAST™ (Genencor); ALCALASE®, SAVINASE®, PRIMASE®, DURAZYM™, POLARZYME®, OVOZYME®, KANNASE®, LIQUANASE®, NEUTRASE®, RELASE® and ESPERASE® (Novozymes); BLAP™ and BLAP™ variants (Henkel Kommanditgesellschaft auf Aktien, Duesseldorf, Germany), and KAP ( B. alkalophilus  subtilisin; Kao Corp., Tokyo, Japan). Various proteases are described in WO95/23221, WO 92/21760, WO 09/149200, WO 09/149144, WO 09/149145, WO 11/072099, WO 10/056640, WO 10/056653, WO 11/140364, WO 12/151534, U.S. Pat. Publ. No. 2008/0090747, and U.S. Pat. Nos. 5,801,039, 5,340,735, 5,500,364, 5,855,625, US RE 34,606, 5,955,340, 5,700,676, 6,312,936, and 6,482,628, and various other patents. In some further embodiments, metalloproteases find use in the present invention, including but not limited to the neutral metalloprotease described in WO 07/044993. 
     In addition, any suitable lipase finds use in the present invention. Suitable lipases include, but are not limited to those of bacterial or fungal origin. Chemically or genetically modified mutants are encompassed by the present invention. Examples of useful lipases include  Humicola lanuginosa  lipase (See e.g., EP 258 068, and EP 305 216),  Rhizomucor miehei  lipase (See e.g., EP 238 023),  Candida  lipase, such as  C. antarctica  lipase (e.g., the  C. antarctica  lipase A or B; See e.g., EP 214 761),  Pseudomonas  lipases such as  P. alcaligenes  lipase and  P. pseudoalcaligenes  lipase (See e.g., EP 218 272),  P. cepacia  lipase (See e.g., EP 331 376),  P. stutzeri  lipase (See e.g., GB 1,372,034),  P. fluorescens  lipase,  Bacillus  lipase (e.g.,  B. subtilis  lipase [Dartois et al., Biochem. Biophys. Acta 1131:253-260 [1993]);  B. stearothermophilus  lipase [See e.g., JP 64/744992]; and  B. pumilus  lipase [See e.g., WO 91/16422]). 
     Furthermore, a number of cloned lipases find use in some embodiments of the present invention, including but not limited to  Penicillium camembertii  lipase (See, Yamaguchi et al., Gene 103:61-67 [1991]),  Geotricum candidum  lipase (See, Schimada et al., J. Biochem., 106:383-388 [1989]), and various  Rhizopus  lipases such as  R. delemar  lipase (See, Hass et al., Gene 109:117-113 [1991]), a  R. niveus  lipase (Kugimiya et al., Biosci. Biotech. Biochem. 56:716-719 [1992]) and  R. oryzae  lipase. 
     Other types of lipase polypeptide enzymes such as cutinases also find use in some embodiments of the present invention, including but not limited to the cutinase derived from  Pseudomonas mendocina  (See, WO 88/09367), and the cutinase derived from  Fusarium solani pisi  (See, WO 90/09446). 
     Additional suitable lipases include commercially available lipases such as M1 LIPASE™, LUMA FAST™, and LIPOMAX™ (Genencor); LIPEX®, LIPOLASE® and LIPOLASE® ULTRA (Novozymes); and LIPASE P™ “Amano” (Amano Pharmaceutical Co. Ltd., Japan). 
     In some embodiments of the present invention, the cleaning compositions of the present invention further comprise lipases at a level from about 0.00001% to about 10% of additional lipase by weight of the composition and the balance of cleaning adjunct materials by weight of composition. In some other embodiments of the present invention, the cleaning compositions of the present invention also comprise lipases at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% lipase by weight of the composition. 
     In some embodiments of the present invention, any suitable amylase finds use in the present invention. In some embodiments, any amylase (e.g., alpha and/or beta) suitable for use in alkaline solutions also find use. Suitable amylases include, but are not limited to those of bacterial or fungal origin. Chemically or genetically modified mutants are included in some embodiments. Amylases that find use in the present invention, include, but are not limited to a-amylases obtained from  B. licheniformis  (See e.g., GB 1,296,839). Additional suitable amylases include those found in WO9510603, WO9526397, WO9623874, WO9623873, WO9741213, WO9919467, WO0060060, WO0029560, WO9923211, WO9946399, WO0060058, WO0060059, WO9942567, WO0114532, WO02092797, WO0166712, WO0188107, WO0196537, WO0210355, WO9402597, WO0231124, WO9943793, WO9943794, WO2004113551, WO2005001064, WO2005003311, WO0164852, WO2006063594, WO2006066594, WO2006066596, WO2006012899, WO2008092919, WO2008000825, WO2005018336, WO2005066338, WO2009140504, WO2005019443, WO2010091221, WO2010088447, WO0134784, WO2006012902, WO2006031554, WO2006136161, WO2008101894, WO2010059413, WO2011098531, WO2011080352, WO2011080353, WO2011080354, WO2011082425, WO2011082429, WO2011076123, WO2011087836, WO2011076897, WO94183314, WO9535382, WO9909183, WO9826078, WO9902702, WO9743424, WO9929876, WO9100353, WO9605295, WO9630481, WO9710342, WO2008088493, WO2009149419, WO2009061381, WO2009100102, WO2010104675, WO2010117511, and WO2010115021. Commercially available amylases that find use in the present invention include, but are not limited to DURAMYL®, TERMAMYL®, FUNGAMYL®, STAINZYME®, STAINZYME PLUS®, STAINZYME ULTRA®, and BAN™ (Novozymes), as well as POWERASE™, RAPIDASE® and MAXAMYL® P (Genencor). 
     In some embodiments of the present invention, the cleaning compositions of the present invention further comprise amylases at a level from about 0.00001% to about 10% of additional amylase by weight of the composition and the balance of cleaning adjunct materials by weight of composition. In some other embodiments of the present invention, the cleaning compositions of the present invention also comprise amylases at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% amylase by weight of the composition. 
     In some further embodiments, any suitable cellulase finds used in the cleaning compositions of the present invention. Suitable cellulases include, but are not limited to those of bacterial or fungal origin. Chemically or genetically modified mutants are included in some embodiments. Suitable cellulases include, but are not limited to  Humicola insolens  cellulases (See e.g., U.S. Pat. No. 4,435,307). Especially suitable cellulases are the cellulases having color care benefits (See e.g., EP 0 495 257). Commercially available cellulases that find use in the present include, but are not limited to CELLUZYME, CELLUCLEAN, CAREZYME (Novozymes), PURADEX AND REVITALENZ (Danisco US Inc.), and KAC-500(B) (Kao Corporation). In some embodiments, cellulases are incorporated as portions or fragments of mature wild-type or variant cellulases, wherein a portion of the N-terminus is deleted (See e.g., U.S. Pat. No. 5,874,276). Additional suitable cellulases include those found in WO2005054475, WO2005056787, U.S. Pat. Nos. 7,449,318, and 7,833,773. In some embodiments, the cleaning compositions of the present invention further comprise cellulases at a level from about 0.00001% to about 10% of additional cellulase by weight of the composition and the balance of cleaning adjunct materials by weight of composition. In some other embodiments of the present invention, the cleaning compositions of the present invention also comprise cellulases at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% cellulase by weight of the composition. 
     Any mannanase suitable for use in detergent compositions also finds use in the present invention. Suitable mannanases include, but are not limited to those of bacterial or fungal origin. Chemically or genetically modified mutants are included in some embodiments. Various mannanases are known which find use in the present invention (See e.g., U.S. Pat. Nos. 6,566,114; 6,602,842; 5,476,775 and 6,440,991, and U.S. Prov. App. Ser. No. 61/739,267; all of which are incorporated herein by reference). Commercially available mannanases that find use in the present invention include, but are not limited to MANNASTAR, PURABRITE, and MANNAWAY. In some embodiments, the cleaning compositions of the present invention further comprise mannanases at a level from about 0.00001% to about 10% of additional mannanase by weight of the composition and the balance of cleaning adjunct materials by weight of composition. In some embodiments of the present invention, the cleaning compositions of the present invention also comprise mannanases at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% mannanase by weight of the composition. 
     In some embodiments, peroxidases are used in combination with hydrogen peroxide or a source thereof (e.g., a percarbonate, perborate or persulfate) in the compositions of the present invention. In some alternative embodiments, oxidases are used in combination with oxygen. Both types of enzymes are used for “solution bleaching” (i.e., to prevent transfer of a textile dye from a dyed fabric to another fabric when the fabrics are washed together in a wash liquor), preferably together with an enhancing agent (See e.g., WO 94/12621 and WO 95/01426). Suitable peroxidases/oxidases include, but are not limited to those of plant, bacterial or fungal origin. Chemically or genetically modified mutants are included in some embodiments. In some embodiments, the cleaning compositions of the present invention further comprise peroxidase and/or oxidase enzymes at a level from about 0.00001% to about 10% of additional peroxidase and/or oxidase by weight of the composition and the balance of cleaning adjunct materials by weight of composition. In some other embodiments of the present invention, the cleaning compositions of the present invention also comprise, peroxidase and/or oxidase enzymes at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% peroxidase and/or oxidase enzymes by weight of the composition. 
     In some embodiments, additional enzymes find use, including but not limited to perhydrolases (See e.g., WO 05/056782). In addition, in some embodiments, mixtures of the above mentioned enzymes are encompassed herein, in particular one or more additional protease, amylase, lipase, mannanase, and/or at least one cellulase. Indeed, it is contemplated that various mixtures of these enzymes will find use in the present invention. It is also contemplated that the varying levels of the metalloprotease polypeptide (s) and one or more additional enzymes may both independently range to about 10%, the balance of the cleaning composition being cleaning adjunct materials. The specific selection of cleaning adjunct materials are readily made by considering the surface, item, or fabric to be cleaned, and the desired form of the composition for the cleaning conditions during use (e.g., through the wash detergent use). 
     Examples of suitable cleaning adjunct materials include, but are not limited to, surfactants, builders, bleaches, bleach activators, bleach catalysts, other enzymes, enzyme stabilizing systems, chelants, optical brighteners, soil release polymers, dye transfer agents, dye transfer inhibiting agents, catalytic materials, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric dispersing agents, clay soil removal agents, structure elasticizing agents, dispersants, suds suppressors, dyes, perfumes, colorants, filler salts, hydrotropes, photoactivators, fluorescers, fabric conditioners, fabric softeners, carriers, hydrotropes, processing aids, solvents, pigments, hydrolyzable surfactants, preservatives, anti-oxidants, anti-shrinkage agents, anti-wrinkle agents, germicides, fungicides, color speckles, silvercare, anti-tarnish and/or anti-corrosion agents, alkalinity sources, solubilizing agents, carriers, processing aids, pigments, and pH control agents (See e.g., U.S. Pat. Nos. 6,610,642; 6,605,458; 5,705,464; 5,710,115; 5,698,504; 5,695,679; 5,686,014 and 5,646,101, all of which are incorporated herein by reference). Embodiments of specific cleaning composition materials are exemplified in detail below. In embodiments in which the cleaning adjunct materials are not compatible with the metalloprotease polypeptides of the present invention in the cleaning compositions, then suitable methods of keeping the cleaning adjunct materials and the protease(s) separated (i.e., not in contact with each other) until combination of the two components is appropriate are used. Such separation methods include any suitable method known in the art (e.g., gelcaps, encapsulation, tablets, physical separation, etc.). 
     In some embodiments, an effective amount of one or more metalloprotease polypeptide (s) provided herein is included in compositions useful for cleaning a variety of surfaces in need of proteinaceous stain removal. Such cleaning compositions include cleaning compositions for such applications as cleaning hard surfaces, fabrics, and dishes. Indeed, in some embodiments, the present invention provides fabric cleaning compositions, while in other embodiments, the present invention provides non-fabric cleaning compositions. Notably, the present invention also provides cleaning compositions suitable for personal care, including oral care (including dentrifices, toothpastes, mouthwashes, etc., as well as denture cleaning compositions), skin, and hair cleaning compositions. It is intended that the present invention encompass detergent compositions in any form (i.e., liquid, granular, bar, semi-solid, gels, emulsions, tablets, capsules, etc.). 
     By way of example, several cleaning compositions wherein the metalloprotease polypeptides of the present invention find use are described in greater detail below. In some embodiments in which the cleaning compositions of the present invention are formulated as compositions suitable for use in laundry machine washing method(s), the compositions of the present invention preferably contain at least one surfactant and at least one builder compound, as well as one or more cleaning adjunct materials preferably selected from organic polymeric compounds, bleaching agents, additional enzymes, suds suppressors, dispersants, lime-soap dispersants, soil suspension and anti-redeposition agents and corrosion inhibitors. In some embodiments, laundry compositions also contain softening agents (i.e., as additional cleaning adjunct materials). The compositions of the present invention also find use in detergent additive products in solid or liquid form. Such additive products are intended to supplement and/or boost the performance of conventional detergent compositions and can be added at any stage of the cleaning process. In some embodiments, the density of the laundry detergent compositions herein ranges from about 400 to about 1200 g/liter, while in other embodiments, it ranges from about 500 to about 950 g/liter of composition measured at 20° C. 
     In embodiments formulated as compositions for use in manual dishwashing methods, the compositions of the invention preferably contain at least one surfactant and preferably at least one additional cleaning adjunct material selected from organic polymeric compounds, suds enhancing agents, group II metal ions, solvents, hydrotropes and additional enzymes. 
     In some embodiments, various cleaning compositions such as those provided in U.S. Pat. No. 6,605,458, find use with the metalloprotease polypeptides of the present invention. Thus, in some embodiments, the compositions comprising at least one metalloprotease polypeptide of the present invention is a compact granular fabric cleaning composition, while in other embodiments, the composition is a granular fabric cleaning composition useful in the laundering of colored fabrics, in further embodiments, the composition is a granular fabric cleaning composition which provides softening through the wash capacity, in additional embodiments, the composition is a heavy duty liquid fabric cleaning composition. In some embodiments, the compositions comprising at least one metalloprotease polypeptide of the present invention are fabric cleaning compositions such as those described in U.S. Pat. Nos. 6,610,642 and 6,376,450. In addition, the metalloprotease polypeptides of the present invention find use in granular laundry detergent compositions of particular utility under European or Japanese washing conditions (See e.g., U.S. Pat. No. 6,610,642). 
     In some alternative embodiments, the present invention provides hard surface cleaning compositions comprising at least one metalloprotease polypeptide provided herein. Thus, in some embodiments, the compositions comprising at least one metalloprotease polypeptide of the present invention is a hard surface cleaning composition such as those described in U.S. Pat. Nos. 6,610,642; 6,376,450, and 6,376,450. 
     In yet further embodiments, the present invention provides dishwashing compositions comprising at least one metalloprotease polypeptide provided herein. Thus, in some embodiments, the compositions comprising at least one metalloprotease polypeptide of the present invention is a hard surface cleaning composition such as those in U.S. Pat. Nos. 6,610,642 and 6,376,450. In some still further embodiments, the present invention provides dishwashing compositions comprising at least one metalloprotease polypeptide provided herein. In some further embodiments, the compositions comprising at least one metalloprotease polypeptide of the present invention comprise oral care compositions such as those in U.S. Pat. Nos. 6,376,450, and 6,376,450. The formulations and descriptions of the compounds and cleaning adjunct materials contained in the aforementioned U.S. Pat. Nos. 6,376,450; 6,605,458; 6,605,458, and 6,610,642, find use with the metalloprotease polypeptides provided herein. 
     The cleaning compositions of the present invention are formulated into any suitable form and prepared by any process chosen by the formulator, non-limiting examples of which are described in U.S. Pat. Nos. 5,879,584; 5,691,297; 5,574,005; 5,569,645; 5,565,422; 5,516,448; 5,489,392, and 5,486,303, all of which are incorporated herein by reference. When a low pH cleaning composition is desired, the pH of such composition is adjusted via the addition of a material such as monoethanolamine or an acidic material such as HCl. 
     In some embodiments, the cleaning compositions of the present invention can be formulated to have an alkaline pH under wash conditions, such as a pH of from about 8.0 to about 12.0, or from about 8.5 to about 11.0, or from about 9.0 to about 11.0. In some embodiments, the cleaning compositions of the present invention can be formulated to have a neutral pH under wash conditions, such as a pH of from about 5.0 to about 8.0, or from about 5.5 to about 8.0, or from about 6.0 to about 8.0, or from about 6.0 to about 7.5. In some embodiments, the neutral pH conditions can be measured when the cleaning composition is dissolved 1:100 (wt:wt) in de-ionised water at 20° C., measured using a conventional pH meter. 
     While not essential for the purposes of the present invention, the non-limiting list of adjuncts illustrated hereinafter are suitable for use in the instant cleaning compositions. In some embodiments, these adjuncts are incorporated for example, to assist or enhance cleaning performance, for treatment of the substrate to be cleaned, or to modify the aesthetics of the cleaning composition as is the case with perfumes, colorants, dyes or the like. It is understood that such adjuncts are in addition to the metalloprotease polypeptides of the present invention. The precise nature of these additional components, and levels of incorporation thereof, will depend on the physical form of the composition and the nature of the cleaning operation for which it is to be used. Suitable adjunct materials include, but are not limited to, surfactants, builders, chelating agents, dye transfer inhibiting agents, deposition aids, dispersants, additional enzymes, and enzyme stabilizers, catalytic materials, bleach activators, bleach boosters, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric dispersing agents, clay soil removal/anti-redeposition agents, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, fabric softeners, carriers, hydrotropes, processing aids and/or pigments. In addition to the disclosure below, suitable examples of such other adjuncts and levels of use are found in U.S. Pat. Nos. 5,576,282, 6,306,812, and 6,326,348, incorporated by reference. The aforementioned adjunct ingredients may constitute the balance of the cleaning compositions of the present invention. 
     In some embodiments, the cleaning compositions according to the present invention comprise an acidifying particle or an amino carboxylic builder. Examples of an amino carboxylic builder include aminocarboxylic acids, salts and derivatives thereof. In some embodiment, the amino carboxylic builder is an aminopolycarboxylic builder, such as glycine-N,N-diacetic acid or derivative of general formula MOOC—CHR-N(CH 2 COOM) 2  where R is C 1-12  alkyl and M is alkali metal. In some embodiments, the amino carboxylic builder can be methylglycine diacetic acid (MGDA), GLDA (glutamic-N,N-diacetic acid), iminodisuccinic acid (IDS), carboxymethyl inulin and salts and derivatives thereof, aspartic acid-N-monoacetic acid (ASMA), aspartic acid-N,N-diacetic acid (ASDA), aspartic acid-N-monopropionic acid (ASMP), iminodisuccinic acid (IDA), N-(2-sulfomethyl) aspartic acid (SMAS), N-(2-sulfoethyl)aspartic acid (SEAS), N-(2-sulfomethyl)glutamic acid (SMGL), N-(2-sulfoethyl) glutamic acid (SEGL), IDS (iminodiacetic acid) and salts and derivatives thereof such as N-methyliminodiacetic acid (MIDA), alpha-alanine-N,N-diacetic acid (alpha-ALDA), serine-N,N-diacetic acid (SEDA), isoserine-N,Ndiacetic acid (ISDA), phenylalanine-N,N-diacetic acid (PHDA), anthranilic acid-N,N-diacetic acid (ANDA), sulfanilic acid-N,N-diacetic acid (SLDA), taurine-N,N-diacetic acid (TUDA) and sulfomethyl-N,N-diacetic acid (SMDA) and alkali metal salts and derivative thereof. In some embodiments, the acidifying particle has a weight geometric mean particle size of from about 400μ to about 1200μ and a bulk density of at least 550 g/L. In some embodiments, the acidifying particle comprises at least about 5% of the builder. 
     In some embodiments, the acidifying particle can comprise any acid, including organic acids and mineral acids. Organic acids can have one or two carboxyls and in some instances up to 15 carbons, especially up to 10 carbons, such as formic, acetic, propionic, capric, oxalic, succinic, adipic, maleic, fumaric, sebacic, malic, lactic, glycolic, tartaric and glyoxylic acids. In some embodiments, the acid is citric acid. Mineral acids include hydrochloric and sulphuric acid. In some instances, the acidifying particle of the invention is a highly active particle comprising a high level of amino carboxylic builder. Sulphuric acid has been found to further contribute to the stability of the final particle. 
     In some embodiments, the cleaning compositions according to the present invention comprise at least one surfactant and/or a surfactant system wherein the surfactant is selected from nonionic surfactants, anionic surfactants, cationic surfactants, ampholytic surfactants, zwitterionic surfactants, semi-polar nonionic surfactants and mixtures thereof. In some low pH cleaning composition embodiments (e.g., compositions having a neat pH of from about 3 to about 5), the composition typically does not contain alkyl ethoxylated sulfate, as it is believed that such surfactant may be hydrolyzed by such compositions the acidic contents. In some embodiments, the surfactant is present at a level of from about 0.1% to about 60%, while in alternative embodiments the level is from about 1% to about 50%, while in still further embodiments the level is from about 5% to about 40%, by weight of the cleaning composition. 
     In some embodiments, the cleaning compositions of the present invention comprise one or more detergent builders or builder systems. In some embodiments incorporating at least one builder, the cleaning compositions comprise at least about 1%, from about 3% to about 60% or even from about 5% to about 40% builder by weight of the cleaning composition. Builders include, but are not limited to, the alkali metal, ammonium and alkanolammonium salts of polyphosphates, alkali metal silicates, alkaline earth and alkali metal carbonates, aluminosilicates, polycarboxylate compounds, ether hydroxypolycarboxylates, copolymers of maleic anhydride with ethylene or vinyl methyl ether, 1, 3, 5-trihydroxy benzene-2, 4, 6-trisulphonic acid, and carboxymethyloxysuccinic acid, the various alkali metal, ammonium and substituted ammonium salts of polyacetic acids such as ethylenediamine tetraacetic acid and nitrilotriacetic acid, as well as polycarboxylates such as mellitic acid, succinic acid, citric acid, oxydisuccinic acid, polymaleic acid, benzene 1,3,5-tricarboxylic acid, carboxymethyloxysuccinic acid, and soluble salts thereof. Indeed, it is contemplated that any suitable builder will find use in various embodiments of the present invention. 
     In some embodiments, the builders form water-soluble hardness ion complexes (e.g., sequestering builders), such as citrates and polyphosphates (e.g., sodium tripolyphosphate and sodium tripolyphospate hexahydrate, potassium tripolyphosphate, and mixed sodium and potassium tripolyphosphate, etc.). It is contemplated that any suitable builder will find use in the present invention, including those known in the art (See e.g., EP 2 100 949). 
     In some embodiments, builders for use herein include phosphate builders and non-phosphate builders. In some embodiments, the builder is a phosphate builder. In some embodiments, the builder is a non-phosphate builder. If present, builders are used in a level of from 0.1% to 80%, or from 5 to 60%, or from 10 to 50% by weight of the composition. In some embodiments the product comprises a mixture of phosphate and non-phosphate builders. 
     Suitable phosphate builders include mono-phosphates, di-phosphates, tri-polyphosphates or oligomeric-poylphosphates, including the alkali metal salts of these compounds, including the sodium salts. In some embodiments, a builder can be sodium tripolyphosphate (STPP). Additionally, the composition can comprise carbonate and/or citrate, preferably citrate that helps to achieve a neutral pH composition of the invention. Other suitable non-phosphate builders include homopolymers and copolymers of polycarboxylic acids and their partially or completely neutralized salts, monomeric polycarboxylic acids and hydroxycarboxylic acids and their salts. In some embodiments, salts of the above mentioned compounds include the ammonium and/or alkali metal salts, i.e. the lithium, sodium, and potassium salts, including sodium salts. Suitable polycarboxylic acids include acyclic, alicyclic, hetero-cyclic and aromatic carboxylic acids, wherein in some embodiments, they can contain at least two carboxyl groups which are in each case separated from one another by, in some instances, no more than two carbon atoms. 
     In some embodiments, the cleaning compositions of the present invention contain at least one chelating agent. Suitable chelating agents include, but are not limited to copper, iron and/or manganese chelating agents and mixtures thereof. In embodiments in which at least one chelating agent is used, the cleaning compositions of the present invention comprise from about 0.1% to about 15% or even from about 3.0% to about 10% chelating agent by weight of the subject cleaning composition. 
     In some still further embodiments, the cleaning compositions provided herein contain at least one deposition aid. Suitable deposition aids include, but are not limited to, polyethylene glycol, polypropylene glycol, polycarboxylate, soil release polymers such as polytelephthalic acid, clays such as kaolinite, montmorillonite, atapulgite, illite, bentonite, halloysite, and mixtures thereof. 
     As indicated herein, in some embodiments, anti-redeposition agents find use in some embodiments of the present invention. In some embodiments, non-ionic surfactants find use. For example, in automatic dishwashing embodiments, non-ionic surfactants find use for surface modification purposes, in particular for sheeting, to avoid filming and spotting and to improve shine. These non-ionic surfactants also find use in preventing the re-deposition of soils. In some embodiments, the anti-redeposition agent is a non-ionic surfactant as known in the art (See e.g., EP 2 100 949). In some embodiments, the non-ionic surfactant can be ethoxylated nonionic surfactants, epoxy-capped poly(oxyalkylated) alcohols and amine oxides surfactants. 
     In some embodiments, the cleaning compositions of the present invention include one or more dye transfer inhibiting agents. Suitable polymeric dye transfer inhibiting agents include, but are not limited to, polyvinylpyrrolidone polymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinyloxazolidones and polyvinylimidazoles or mixtures thereof. In embodiments in which at least one dye transfer inhibiting agent is used, the cleaning compositions of the present invention comprise from about 0.0001% to about 10%, from about 0.01% to about 5%, or even from about 0.1% to about 3% by weight of the cleaning composition. 
     In some embodiments, silicates are included within the compositions of the present invention. In some such embodiments, sodium silicates (e.g., sodium disilicate, sodium metasilicate, and crystalline phyllosilicates) find use. In some embodiments, silicates are present at a level of from about 1% to about 20%. In some embodiments, silicates are present at a level of from about 5% to about 15% by weight of the composition. 
     In some still additional embodiments, the cleaning compositions of the present invention also contain dispersants. Suitable water-soluble organic materials include, but are not limited to the homo- or co-polymeric acids or their salts, in which the polycarboxylic acid comprises at least two carboxyl radicals separated from each other by not more than two carbon atoms. 
     In some further embodiments, the enzymes used in the cleaning compositions are stabilized by any suitable technique. In some embodiments, the enzymes employed herein are stabilized by the presence of water-soluble sources of calcium and/or magnesium ions in the finished compositions that provide such ions to the enzymes. In some embodiments, the enzyme stabilizers include oligosaccharides, polysaccharides, and inorganic divalent metal salts, including alkaline earth metals, such as calcium salts, such as calcium formate. It is contemplated that various techniques for enzyme stabilization will find use in the present invention. For example, in some embodiments, the enzymes employed herein are stabilized by the presence of water-soluble sources of zinc (II), calcium (II) and/or magnesium (II) ions in the finished compositions that provide such ions to the enzymes, as well as other metal ions (e.g., barium (II), scandium (II), iron (II), manganese (II), aluminum (III), Tin (II), cobalt (II), copper (II), nickel (II), and oxovanadium (IV). Chlorides and sulfates also find use in some embodiments of the present invention. Examples of suitable oligosaccharides and polysaccharides (e.g., dextrins) are known in the art (See e.g., WO 07/145964). In some embodiments, reversible protease inhibitors also find use, such as boron-containing compounds (e.g., borate, 4-formyl phenyl boronic acid) and/or a tripeptide aldehyde find use to further improve stability, as desired. 
     In some embodiments, bleaches, bleach activators and/or bleach catalysts are present in the compositions of the present invention. In some embodiments, the cleaning compositions of the present invention comprise inorganic and/or organic bleaching compound(s). Inorganic bleaches include, but are not limited to perhydrate salts (e.g., perborate, percarbonate, perphosphate, persulfate, and persilicate salts). In some embodiments, inorganic perhydrate salts are alkali metal salts. In some embodiments, inorganic perhydrate salts are included as the crystalline solid, without additional protection, although in some other embodiments, the salt is coated. Any suitable salt known in the art finds use in the present invention (See e.g., EP 2 100 949). 
     In some embodiments, bleach activators are used in the compositions of the present invention. Bleach activators are typically organic peracid precursors that enhance the bleaching action in the course of cleaning at temperatures of 60° C. and below. Bleach activators suitable for use herein include compounds which, under perhydrolysis conditions, give aliphatic peroxoycarboxylic acids having preferably from about 1 to about 10 carbon atoms, in particular from about 2 to about 4 carbon atoms, and/or optionally substituted perbenzoic acid. Additional bleach activators are known in the art and find use in the present invention (See e.g., EP 2 100 949). 
     In addition, in some embodiments and as further described herein, the cleaning compositions of the present invention further comprise at least one bleach catalyst. In some embodiments, the manganese triazacyclononane and related complexes find use, as well as cobalt, copper, manganese, and iron complexes. Additional bleach catalysts find use in the present invention (See e.g., U.S. Pat. Nos. 4,246,612, 5,227,084, 4,810410, WO 99/06521, and EP 2 100 949). 
     In some embodiments, the cleaning compositions of the present invention contain one or more catalytic metal complexes. In some embodiments, a metal-containing bleach catalyst finds use. In some embodiments, the metal bleach catalyst comprises a catalyst system comprising a transition metal cation of defined bleach catalytic activity, (e.g., copper, iron, titanium, ruthenium, tungsten, molybdenum, or manganese cations), an auxiliary metal cation having little or no bleach catalytic activity (e.g., zinc or aluminum cations), and a sequestrate having defined stability constants for the catalytic and auxiliary metal cations, particularly ethylenediaminetetraacetic acid, ethylenediaminetetra (methylenephosphonic acid) and water-soluble salts thereof are used (See e.g., U.S. Pat. No. 4,430,243). In some embodiments, the cleaning compositions of the present invention are catalyzed by means of a manganese compound. Such compounds and levels of use are well known in the art (See e.g., U.S. Pat. No. 5,576,282). In additional embodiments, cobalt bleach catalysts find use in the cleaning compositions of the present invention. Various cobalt bleach catalysts are known in the art (See e.g., U.S. Pat. Nos. 5,597,936 and 5,595,967) and are readily prepared by known procedures. 
     In some additional embodiments, the cleaning compositions of the present invention include a transition metal complex of a macropolycyclic rigid ligand (MRL). As a practical matter, and not by way of limitation, in some embodiments, the compositions and cleaning processes provided by the present invention are adjusted to provide on the order of at least one part per hundred million of the active MRL species in the aqueous washing medium, and in some embodiments, provide from about 0.005 ppm to about 25 ppm, more preferably from about 0.05 ppm to about 10 ppm, and most preferably from about 0.1 ppm to about 5 ppm, of the MRL in the wash liquor. 
     In some embodiments, transition-metals in the instant transition-metal bleach catalyst include, but are not limited to manganese, iron and chromium. MRLs also include, but are not limited to special ultra-rigid ligands that are cross-bridged (e.g., 5,12-diethyl-1,5,8,12-tetraazabicyclo[6.6.2]hexadecane). Suitable transition metal MRLs are readily prepared by known procedures (See e.g., WO 2000/32601, and U.S. Pat. No. 6,225,464). 
     In some embodiments, the cleaning compositions of the present invention comprise metal care agents. Metal care agents find use in preventing and/or reducing the tarnishing, corrosion, and/or oxidation of metals, including aluminum, stainless steel, and non-ferrous metals (e.g., silver and copper). Suitable metal care agents include those described in EP 2 100 949, WO 9426860 and WO 94/26859). In some embodiments, the metal care agent is a zinc salt. In some further embodiments, the cleaning compositions of the present invention comprise from about 0.1% to about 5% by weight of one or more metal care agent. 
     In some embodiments, the cleaning composition is a high density liquid (HDL) composition having a variant metalloprotease polypeptide protease. The HDL liquid laundry detergent can comprise a detersive surfactant (10%-40%) comprising anionic detersive surfactant (selected from a group of linear or branched or random chain, substituted or unsubstituted alkyl sulphates, alkyl sulphonates, alkyl alkoxylated sulphate, alkyl phosphates, alkyl phosphonates, alkyl carboxylates, and/or mixtures thereof); and optionally non-ionic surfactant (selected from a group of linear or branched or random chain, substituted or unsubstituted alkyl alkoxylated alcohol, for example a C 8 -C 18  alkyl ethoxylated alcohol and/or C 6 -C 12  alkyl phenol alkoxylates), optionally wherein the weight ratio of anionic detersive surfactant (with a hydrophilic index (HIc) of from 6.0 to 9) to non-ionic detersive surfactant is greater than 1:1. 
     The composition can comprise optionally, a surfactancy boosting polymer consisting of amphiphilic alkoxylated grease cleaning polymers (selected from a group of alkoxylated polymers having branched hydrophilic and hydrophobic properties, such as alkoxylated polyalkylenimines in the range of 0.05 wt %-10 wt %) and/or random graft polymers (typically comprising of hydrophilic backbone comprising monomers selected from the group consisting of: unsaturated C 1 -C 6  carboxylic acids, ethers, alcohols, aldehydes, ketones, esters, sugar units, alkoxy units, maleic anhydride, saturated polyalcohols such as glycerol, and mixtures thereof; and hydrophobic side chain(s) selected from the group consisting of: C 4 -C 25  alkyl group, polypropylene, polybutylene, vinyl ester of a saturated C-C 6  mono-carboxylic acid, C 1 -C 6  alkyl ester of acrylic or methacrylic acid, and mixtures thereof. 
     The composition can comprise additional polymers such as soil release polymers (include anionically end-capped polyesters, for example SRP1, polymers comprising at least one monomer unit selected from saccharide, dicarboxylic acid, polyol and combinations thereof, in random or block configuration, ethylene terephthalate-based polymers and co-polymers thereof in random or block configuration, for example Repel-o-tex SF, SF-2 and SRP6, Texcare SRA100, SRA300, SRN100, SRN170, SRN240, SRN300 and SRN325, Marloquest SL), anti-redeposition polymers (0.1 wt % to 10 wt %, include carboxylate polymers, such as polymers comprising at least one monomer selected from acrylic acid, maleic acid (or maleic anhydride), fumaric acid, itaconic acid, aconitic acid, mesaconic acid, citraconic acid, methylenemalonic acid, and any mixture thereof, vinylpyrrolidone homopolymer, and/or polyethylene glycol, molecular weight in the range of from 500 to 100,000 Da); cellulosic polymer (including those selected from alkyl cellulose, alkyl alkoxyalkyl cellulose, carboxyalkyl cellulose, alkyl carboxyalkyl cellulose examples of which include carboxymethyl cellulose, methyl cellulose, methyl hydroxyethyl cellulose, methyl carboxymethyl cellulose, and mixtures thereof) and polymeric carboxylate (such as maleate/acrylate random copolymer or polyacrylate homopolymer). 
     The composition can further comprise saturated or unsaturated fatty acid, preferably saturated or unsaturated C 12 -C 24  fatty acid (0 wt % to 10 wt %); deposition aids (examples for which include polysaccharides, preferably cellulosic polymers, poly diallyl dimethyl ammonium halides (DADMAC), and co-polymers of DAD MAC with vinyl pyrrolidone, acrylamides, imidazoles, imidazolinium halides, and mixtures thereof, in random or block configuration, cationic guar gum, cationic cellulose such as cationic hydoxyethyl cellulose, cationic starch, cationic polyacylamides, and mixtures thereof. 
     The composition can further comprise dye transfer inhibiting agents examples of which include manganese phthalocyanine, peroxidases, polyvinylpyrrolidone polymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinyloxazolidones and polyvinylimidazoles and/or mixtures thereof; chelating agents examples of which include ethylene-diamine-tetraacetic acid (EDTA); diethylene triamine penta methylene phosphonic acid (DTPMP); hydroxy-ethane diphosphonic acid (HEDP); ethylenediamine N,N′-disuccinic acid (EDDS); methyl glycine diacetic acid (MGDA); diethylene triamine penta acetic acid (DTPA); propylene diamine tetracetic acid (PDT A); 2-hydroxypyridine-N-oxide (HPNO); or methyl glycine diacetic acid (MGDA); glutamic acid N,N-diacetic acid (N,N-dicarboxymethyl glutamic acid tetrasodium salt (GLDA); nitrilotriacetic acid (NTA); 4,5-dihydroxy-m-benzenedisulfonic acid; citric acid and any salts thereof; N-hydroxyethylethylenediaminetri-acetic acid (HEDTA), triethylenetetraaminehexaacetic acid (TTHA), N-hydroxyethyliminodiacetic acid (HEIDA), dihydroxyethylglycine (DHEG), ethylenediaminetetrapropionic acid (EDTP) and derivatives thereof. 
     The composition can further comprise enzymes (0.01 wt % active enzyme to 0.03 wt % active enzyme) selected from a group of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, and any mixture thereof. The composition may comprise an enzyme stabilizer (examples of which include polyols such as propylene glycol or glycerol, sugar or sugar alcohol, lactic acid, reversible protease inhibitor, boric acid, a boric acid derivative, e.g., an aromatic borate ester, or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid, peptides or formate). 
     The composition can further comprise silicone or fatty-acid based suds suppressors; heuing dyes, calcium and magnesium cations, visual signaling ingredients, anti-foam (0.001 wt % to about 4.0 wt %), and/or structurant/thickener (0.01 wt % to 5 wt %, selected from the group consisting of diglycerides and triglycerides, ethylene glycol distearate, microcrystalline cellulose, cellulose based materials, microfiber cellulose, biopolymers, xanthan gum, gellan gum, and mixtures thereof). 
     Suitable detersive surfactants also include cationic detersive surfactants (selected from a group of alkyl pyridinium compounds, alkyl quarternary ammonium compounds, alkyl quarternary phosphonium compounds, alkyl ternary sulphonium compounds, and/or mixtures thereof); zwitterionic and/or amphoteric detersive surfactants (selected from a group of alkanolamine sulpho-betaines); ampholytic surfactants; semi-polar non-ionic surfactants and mixtures thereof. 
     The composition can be any liquid form, for example a liquid or gel form, or any combination thereof. The composition may be in any unit dose form, for example a pouch. 
     In some embodiments, the cleaning composition is a high density powder (HDD) composition having a variant metalloprotease polypeptide protease. The HDD powder laundry detergent can comprise a detersive surfactant including anionic detersive surfactants (selected from a group of linear or branched or random chain, substituted or unsubstituted alkyl sulphates, alkyl sulphonates, alkyl alkoxylated sulphate, alkyl phosphates, alkyl phosphonates, alkyl carboxylates and/or mixtures thereof), non-ionic detersive surfactant (selected from a group of linear or branched or random chain, substituted or unsubstituted C 8 -C 18  alkyl ethoxylates, and/or C 6 -C 12  alkyl phenol alkoxylates), cationic detersive surfactants (selected from a group of alkyl pyridinium compounds, alkyl quaternary ammonium compounds, alkyl quaternary phosphonium compounds, alkyl ternary sulphonium compounds, and mixtures thereof), zwitterionic and/or amphoteric detersive surfactants (selected from a group of alkanolamine sulpho-betaines); ampholytic surfactants; semi-polar non-ionic surfactants and mixtures thereof builders (phosphate free builders [for example zeolite builders examples of which include zeolite A, zeolite X, zeolite P and zeolite MAP in the range of 0 wt % to less than 10 wt %]; phosphate builders [examples of which include sodium tri-polyphosphate in the range of 0 wt % to less than 10 wt %]; citric acid, citrate salts and nitrilotriacetic acid or salt thereof in the range of less than 15 wt %); silicate salt (sodium or potassium silicate or sodium meta-silicate in the range of 0 wt % to less than 10 wt %, or layered silicate (SKS-6)); carbonate salt (sodium carbonate and/or sodium bicarbonate in the range of 0 wt % to less than 10 wt %); and bleaching agents (photobleaches, examples of which include sulfonated zinc phthalocyanines, sulfonated aluminum phthalocyanines, xanthenes dyes, and mixtures thereof; hydrophobic or hydrophilic bleach activators (examples of which include dodecanoyl oxybenzene sulfonate, decanoyl oxybenzene sulfonate, decanoyl oxybenzoic acid or salts thereof, 3,5,5-trimethy hexanoyl oxybenzene sulfonate, tetraacetyl ethylene diamine-TAED, and nonanoyloxybenzene sulfonate-NOB S, nitrile quats, and mixtures thereof; hydrogen peroxide; sources of hydrogen peroxide (inorganic perhydrate salts examples of which include mono or tetra hydrate sodium salt of perborate, percarbonate, persulfate, perphosphate, or persilicate); preformed hydrophilic and/or hydrophobic peracids (selected from a group consisting of percarboxylic acids and salts, percarbonic acids and salts, perimidic acids and salts, peroxymonosulfuric acids and salts) &amp; mixtures thereof and/or bleach catalyst (such as imine bleach boosters examples of which include iminium cations and polyions; iminium zwitterions; modified amines; modified amine oxides; N-sulphonyl imines; N-phosphonyl imines; N-acyl imines; thiadiazole dioxides; perfluoroimines; cyclic sugar ketones and mixtures thereof; metal-containing bleach catalyst for example copper, iron, titanium, ruthenium, tungsten, molybdenum, or manganese cations along with an auxiliary metal cations such as zinc or aluminum and a sequestrate such as ethylenediaminetetraacetic acid, ethylenediaminetetra(methylenephos-phonic acid) and water-soluble salts thereof). 
     The composition can further comprise enzymes selected from a group of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases and any mixture thereof. 
     The composition can further comprise additional detergent ingredients including perfume microcapsules, starch encapsulated perfume accord, hueing agents, additional polymers including fabric integrity and cationic polymers, dye lock ingredients, fabric-softening agents, brighteners (for example C.I. Fluorescent brighteners), flocculating agents, chelating agents, alkoxylated polyamines, fabric deposition aids, and/or cyclodextrin. 
     In some embodiments, the cleaning composition is an automatic dishwashing (ADW) detergent composition having a metalloprotease of the present invention. The ADW detergent composition can comprise two or more non-ionic surfactants selected from a group of ethoxylated non-ionic surfactants, alcohol alkoxylated surfactants, epoxy-capped poly(oxyalkylated) alcohols, or amine oxide surfactants present in amounts from 0 to 10% by weight; builders in the range of 5-60% comprising either phosphate (mono-phosphates, di-phosphates, tri-polyphosphates or oligomeric-poylphosphates, preferred sodium tripolyphosphate-STPP or phosphate-free builders [amino acid based compounds, examples of which include MGDA (methyl-glycine-diacetic acid), and salts and derivatives thereof, GLDA (glutamic-N,Ndiacetic acid) and salts and derivatives thereof, IDS (iminodisuccinic acid) and salts and derivatives thereof, carboxy methyl inulin and salts and derivatives thereof and mixtures thereof, nitrilotriacetic acid (NTA), diethylene triamine penta acetic acid (DTPA), B-alaninediacetic acid (B-ADA) and their salts], homopolymers and copolymers of poly-carboxylic acids and their partially or completely neutralized salts, monomeric polycarboxylic acids and hydroxycarboxylic acids and their salts in the range of 0.5% to 50% by weight; sulfonated/carboxylated polymers (provide dimensional stability to the product) in the range of about 0.1% to about 50% by weight; drying aids in the range of about 0.1% to about 10% by weight (selected from polyesters, especially anionic polyesters optionally together with further monomers with 3 to 6 functionalities which are conducive to polycondensation, specifically acid, alcohol or ester functionalities, polycarbonate-, polyurethane- and/or polyurea-polyorganosiloxane compounds or precursor compounds thereof of the reactive cyclic carbonate and urea type); silicates in the range from about 1% to about 20% by weight (sodium or potassium silicates for example sodium disilicate, sodium meta-silicate and crystalline phyllosilicates); bleach-inorganic (for example perhydrate salts such as perborate, percarbonate, perphosphate, persulfate and persilicate salts) and organic (for example organic peroxyacids including diacyl and tetraacylperoxides, especially diperoxydodecanedioc acid, diperoxytetradecanedioc acid, and diperoxyhexadecanedioc acid); bleach activators-organic peracid precursors in the range from about 0.1% to about 10% by weight; bleach catalysts (selected from manganese triazacyclononane and related complexes, Co, Cu, Mn and Fe bispyridylamine and related complexes, and pentamine acetate cobalt(III) and related complexes); metal care agents in the range from about 0.1% to 5% by weight (selected from benzatriazoles, metal salts and complexes, and/or silicates); enzymes in the range from about 0.01 to 5.0 mg of active enzyme per gram of automatic dishwashing detergent composition (acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, and any mixture thereof); and enzyme stabilizer components (selected from oligosaccharides, polysaccharides and inorganic divalent metal salts). 
     The metalloproteases are normally incorporated into the detergent composition at a level of from 0.000001% to 5% of enzyme protein by weight of the composition, or from 0.00001% to 2%, or from 0.0001% to 1%, or from 0.001% to 0.75% of enzyme protein by weight of the composition. 
     Metalloprotease Polypeptides of the Present Invention for Use in Animal Feed 
     In a further aspect of the invention, the metalloprotease polypeptides of the present invention can be used as a component of an animal feed composition, animal feed additive and/or pet food comprising a metalloprotease and variants thereof. The present invention further relates to a method for preparing such an animal feed composition, animal feed additive composition and/or pet food comprising mixing the metalloprotease polypeptide with one or more animal feed ingredients and/or animal feed additive ingredients and/or pet food ingredients. Furthermore, the present invention relates to the use of the metalloprotease polypeptide in the preparation of an animal feed composition and/or animal feed additive composition and/or pet food. 
     The term “animal” includes all non-ruminant and ruminant animals. In a particular embodiment, the animal is a non-ruminant animal, such as a horse and a mono-gastric animal. Examples of mono-gastric animals include, but are not limited to, pigs and swine, such as piglets, growing pigs, sows; poultry such as turkeys, ducks, chicken, broiler chicks, layers; fish such as salmon, trout, tilapia, catfish and carps; and crustaceans such as shrimps and prawns. In a further embodiment the animal is a ruminant animal including, but not limited to, cattle, young calves, goats, sheep, giraffes, bison, moose, elk, yaks, water buffalo, deer, camels, alpacas, llamas, antelope, pronghorn and nilgai. 
     In the present context, it is intended that the term “pet food” is understood to mean a food for a household animal such as, but not limited to, dogs, cats, gerbils, hamsters, chinchillas, fancy rats, guinea pigs; avian pets, such as canaries, parakeets, and parrots; reptile pets, such as turtles, lizards and snakes; and aquatic pets, such as tropical fish and frogs. 
     The terms “animal feed composition,” “feedstuff” and “fodder” are used interchangeably and can comprise one or more feed materials selected from the group comprising a) cereals, such as small grains (e.g., wheat, barley, rye, oats and combinations thereof) and/or large grains such as maize or sorghum; b) by products from cereals, such as corn gluten meal, Distillers Dried Grain Solubles (DDGS) (particularly corn based Distillers Dried Grain Solubles (cDDGS), wheat bran, wheat middlings, wheat shorts, rice bran, rice hulls, oat hulls, palm kernel, and citrus pulp; c) protein obtained from sources such as soya, sunflower, peanut, lupin, peas, fava beans, cotton, canola, fish meal, dried plasma protein, meat and bone meal, potato protein, whey, copra, sesame; d) oils and fats obtained from vegetable and animal sources; e) minerals and vitamins. 
     Metalloprotease Polypeptides of the Present Invention for Use in Textile Desizing 
     Also contemplated are compositions and methods of treating fabrics (e.g., to desize a textile) using a metalloprotease polypeptide of the present invention. Fabric-treating methods are well known in the art (see, e.g., U.S. Pat. No. 6,077,316). For example, the feel and appearance of a fabric can be improved by a method comprising contacting the fabric with a metalloprotease in a solution. The fabric can be treated with the solution under pressure. 
     A metalloprotease of the present invention can be applied during or after the weaving of a textile, or during the desizing stage, or one or more additional fabric processing steps. During the weaving of textiles, the threads are exposed to considerable mechanical strain. Prior to weaving on mechanical looms, warp yarns are often coated with sizing starch or starch derivatives to increase their tensile strength and to prevent breaking. A metalloprotease of the present invention can be applied during or after the weaving to remove these sizing starch or starch derivatives. After weaving, the metalloprotease can be used to remove the size coating before further processing the fabric to ensure a homogeneous and wash-proof result. 
     A metalloprotease of the present invention can be used alone or with other desizing chemical reagents and/or desizing enzymes to desize fabrics, including cotton-containing fabrics, as detergent additives, e.g., in aqueous compositions. An amylase also can be used in compositions and methods for producing a stonewashed look on indigo-dyed denim fabric and garments. For the manufacture of clothes, the fabric can be cut and sewn into clothes or garments, which are afterwards finished. In particular, for the manufacture of denim jeans, different enzymatic finishing methods have been developed. The finishing of denim garment normally is initiated with an enzymatic desizing step, during which garments are subjected to the action of proteolytic enzymes to provide softness to the fabric and make the cotton more accessible to the subsequent enzymatic finishing steps. The metalloprotease can be used in methods of finishing denim garments (e.g., a “bio-stoning process”), enzymatic desizing and providing softness to fabrics, and/or finishing process. 
     Metalloprotease Polypeptides of the Present Invention for Use in Paper Pulp Bleaching 
     The metalloprotease polypeptides described herein find further use in the enzyme aided bleaching of paper pulps such as chemical pulps, semi-chemical pulps, kraft pulps, mechanical pulps or pulps prepared by the sulfite method. In general terms, paper pulps are incubated with a metalloprotease polypeptide of the present invention under conditions suitable for bleaching the paper pulp. 
     In some embodiments, the pulps are chlorine free pulps bleached with oxygen, ozone, peroxide or peroxyacids. In some embodiments, the metalloprotease polypeptides are used in enzyme aided bleaching of pulps produced by modified or continuous pulping methods that exhibit low lignin contents. In some other embodiments, the metalloprotease polypeptides are applied alone or preferably in combination with xylanase and/or endoglucanase and/or alpha-galactosidase and/or cellobiohydrolase enzymes. 
     Metalloprotease Polypeptides of the Present Invention for Use in Protein Degradation 
     The metalloprotease polypeptides described herein find further use in the enzyme aided removal of proteins from animals and their subsequent degradation or disposal, such as feathers, skin, hair, hide, and the like. In some instances, immersion of the animal carcass in a solution comprising a metalloprotease polypeptide of the present invention can act to protect the skin from damage in comparison to the traditional immersion in scalding water or the defeathering process. In one embodiment, feathers can be sprayed with an isolated metalloprotase polypeptide of the present invention under conditions suitable for digesting or initiating degradation of the plumage. In some embodiments, a metalloprotease of the present invention can be used, as above, in combination with an oxidizing agent. 
     In some embodiments, removal of the oil or fat associated with raw feathers is assisted by using a metalloprotease polypeptide of the present invention. In some embodiments, the metalloprotease polypeptides are used in compositions for cleaning the feathers as well as to sanitize and partially dehydrate the fibers. In some other embodiments, the metalloprotease polypeptides are applied in a wash solution in combination with 95% ethanol or other polar organic solvent with or without a surfactant at about 0.5% (v/v). 
     In yet other embodiments, the disclosed metalloprotease polypeptides find use in recovering protein from plumage. The disclosed metalloprotease polypeptides may be used alone or in combination in suitable feather processing and proteolytic methods, such as those disclosed in PCT/EP2013/065362, PCT/EP2013/065363, and PCT/EP2013/065364, which are hereby incorporated by reference. In some embodiments, the recovered protein can be subsequently used in animal or fish feed. 
     EXPERIMENTAL 
     The claimed invention is described in further detail in the following examples which are not in any way intended to limit the scope of the invention as claimed. 
     Example 1.1 
     Cloning of  Paenibacillus  sp. Metalloprotease PspPro3 
     A strain of  Paenibacillus  sp. was selected as a potential source for enzymes which may be useful for various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hours. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the  Paenibacillus  sp. strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina&#39;s next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in  Paenibacillus  sp. encodes a metalloprotease and the sequence of this gene, called PspPro3, is provided in SEQ ID NO: 1. The corresponding protein encoded by the PspPro3 gene is shown in SEQ ID NO: 2. At the N-terminus, the protein has a signal peptide with a length of 26 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PspPro3 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the  Paenibacillus polymyxa  Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PspPro3 protein is shown on SEQ ID NO: 3. 
     The nucleotide sequence of the PspPro3 gene isolated from  Paenibacillus  sp. is set forth as SEQ ID NO: 1. The sequence encoding the predicted native signal peptide is shown in italics: 
     
       
         
           
               
            
               
                 
                   ATGTTAATGAAAAAAGTATGGGTTTCGCTTCTTGGAGGAGCGATGTTAT 
                 
               
               
                   
               
               
                   TAGGGTCTGTAGCGTCTGGTGCA TCAGCAGCGGAGAGTTCCGTTTCGGG 
               
               
                   
               
               
                 GCCGGCTCAGCTTACGCCAACCTTCCATGCCGAACAATGGAAAGCACCT 
               
               
                   
               
               
                 TCATCGGTATCGGGTGATGACATCGTATGGAGCTATTTAAATCGGCAAA 
               
               
                   
               
               
                 AGAAAACGTTGCTGGGTACGGACAGCACCAGTGTCCGTGATCAATTCCG 
               
               
                   
               
               
                 TATCGTAGATCGCACAAGCGACAAATCCGGCGTGAGCCATTATCGGCTG 
               
               
                   
               
               
                 AAGCAATATGTAAACGGAATTCCCGTATATGGAGCTGAACAGACCATTC 
               
               
                   
               
               
                 ATGTGGGCAAATCCGGTGAAGTGACCTCTTATCTGGGAGCCGTGATTAC 
               
               
                   
               
               
                 TGAGGATCAGCAAGAAGAAGCTACGCAAGGTACAACTCCGAAAATCAGC 
               
               
                   
               
               
                 GCTTCTGAAGCGGTCCATACCGCATATCAGGAGGCAGCTACACGGGTTC 
               
               
                   
               
               
                 AAGCCCTCCCTACCTCCGATGATACGATTTCTAAAGATGCGGAGGAGCC 
               
               
                   
               
               
                 AAGCAGTGTAAGCAAAGACACTTACTCCGAAGCAGCTAACAACGGAAAA 
               
               
                   
               
               
                 ACGAGTTCTGTTGAAAAGGACAAGCTCAGCCTTGAGAAAGCGGCTGACC 
               
               
                   
               
               
                 TGAAAGATAGCAAAATTGAAGCGGTGGAGGCAGAGCCAAACTCCATTGC 
               
               
                   
               
               
                 CAAAATCGCCAACCTGCAGCCTGAGGTAGATCCTAAAGCCGAACTATAT 
               
               
                   
               
               
                 TTCTATGCGAAGGGCGATGCATTGCAGCTGGTTTATGTGACTGAGGTTA 
               
               
                   
               
               
                 ATATTTTGCAGCCTGCGCCGCTGCGTACACGCTACATCATTGACGCCAA 
               
               
                   
               
               
                 TGATGGCAAAATCGTATCCCAGTATGACATCATTAATGAAGCGACAGGC 
               
               
                   
               
               
                 ACAGGCAAAGGTGTACTCGGTGATACCAAAACATTCAACACTACTGCTT 
               
               
                   
               
               
                 CCGGCAGCAGCTACCAGTTAAGAGATACGACTCGCGGGAATGGAATCGT 
               
               
                   
               
               
                 GACTTACACGGCCTCCAACCGTCAAAGCATCCCAGGTACGATCCTGACC 
               
               
                   
               
               
                 GATGCCGATAACGTATGGAATGATCCAGCCGGCGTGGATGCCCACGCTT 
               
               
                   
               
               
                 ATGCAGCCAAAACCTATGATTATTATAAGGAAAAGTTCAATCGCAACAG 
               
               
                   
               
               
                 CATTGACGGACGAGGCCTGCAGCTCCGTTCGACAGTTCATTACGGCAAT 
               
               
                   
               
               
                 CGTTACAACAACGCCTTCTGGAACGGCTCCCAAATGACTTATGGAGACG 
               
               
                   
               
               
                 GAGACGGCACCACATTTATCGCTTTTAGCGGTGATCCGGATGTAGTTGG 
               
               
                   
               
               
                 TCATGAACTCACACACGGTGTTACGGAGTATACTTCCAATTTGGAATAT 
               
               
                   
               
               
                 TACGGAGAATCCGGTGCGTTGAACGAGGCCTTCTCGGACATCATCGGCA 
               
               
                   
               
               
                 ATGACATCCAGCGTAAAAACTGGCTTGTAGGCGATGATATTTACACGCC 
               
               
                   
               
               
                 ACGCATTGCGGGTGATGCACTTCGTTCTATGTCCAATCCTACGCTGTAC 
               
               
                   
               
               
                 GATCAACCGGATCACTATTCGAACTTGTACAGAGGCAGCTCCGATAACG 
               
               
                   
               
               
                 GCGGCGTTCATACGAACAGCGGTATTATAAATAAAGCCTATTATCTGTT 
               
               
                   
               
               
                 GGCACAAGGCGGCACCTTCCATGGTGTAACTGTCAATGGGATTGGCCGC 
               
               
                   
               
               
                 GATGCAGCGGTTCAAATTTACTACAGCGCCTTTACGAACTACCTGACTT 
               
               
                   
               
               
                 CTTCTTCTGACTTCTCCAATGCACGTGATGCCGTTGTACAAGCGGCAAA 
               
               
                   
               
               
                 AGATCTCTACGGCGCGAGCTCGGCACAAGCTACCGCAGCAGCCAAATCT 
               
               
                   
               
               
                 TTTGATGCTGTAGGCGTTAAC 
               
            
           
         
       
     
     The amino acid sequence of the PspPro3 precursor protein is set forth as SEQ ID NO: 2. The predicted signal peptide is shown in italics, and the predicted pro-peptide is shown in underlined text: 
     
       
         
           
               
            
               
                 
                   MLMKKVWVSLLGGAMLLGSVASGASA 
                   AESSVSGPAQLTPTFHAEQWKAP 
                 
               
               
                   
               
               
                 
                   SSVSGDDIVWSYLNRQKKTLLGTDSTSVRDQFRIVDRTSDKSGVSHYRL 
                 
               
               
                   
               
               
                 
                   KQYVNGIPVYGAEQTIHVGKSGEVTSYLGAVITEDQQEEATQGTTPKIS 
                 
               
               
                   
               
               
                 
                   ASEAVHTAYQEAATRVQALPTSDDTISKDAEEPSSVSKDTYSEAANNGK 
                 
               
               
                   
               
               
                 
                   TSSVEKDKLSLEKAADLKDSKIEAVEAEPNSIAKIANLQPEVDPKAELY 
                 
               
               
                   
               
               
                   FYAKGDALQLVYVTEVNILQPAPLRTRYIIDANDGKIVSQYDIINE ATG 
               
               
                   
               
               
                 TGKGVLGDTKTFNTTASGSSYQLRDTTRGNGIVTYTASNRQSIPGTILT 
               
               
                   
               
               
                 DADNVWNDPAGVDAHAYAAKTYDYYKEKFNRNSIDGRGLQLRSTVHYGN 
               
               
                   
               
               
                 RYNNAFWNGSQMTYGDGDGTTFIAFSGDPDVVGHELTHGVTEYTSNLEY 
               
               
                   
               
               
                 YGESGALNEAFSDIIGNDIQRKNWLVGDDIYTPRIAGDALRSMSNPTLY 
               
               
                   
               
               
                 DQPDHYSNLYRGSSDNGGVHTNSGIINKAYYLLAQGGTFHGVTVNGIGR 
               
               
                   
               
               
                 DAAVQIYYSAFTNYLTSSSDFSNARDAVVQAAKDLYGASSAQATAAAKS 
               
               
                   
               
               
                 FDAVGVN 
               
            
           
         
       
     
     The amino acid sequence of the predicted mature form of PspPro3 is set forth as SEQ ID NO: 3: 
     
       
         
           
               
            
               
                 ATGTGKGVLGDTKTFNTTASGSSYQLRDTTRGNGIVTYTASNRQSIPGTI 
               
               
                   
               
               
                 LTDADNVWNDPAGVDAHAYAAKTYDYYKEKFNRNSIDGRGLQLRSTVHYG 
               
               
                   
               
               
                 NRYNNAFWNGSQMTYGDGDGTTFIAFSGDPDVVGHELTHGVTEYTSNLEY 
               
               
                   
               
               
                 YGESGALNEAFSDIIGNDIQRKNWLVGDDIYTPRIAGDALRSMSNPTLYD 
               
               
                   
               
               
                 QPDHYSNLYRGSSDNGGVHTNSGIINKAYYLLAQGGTFHGVTVNGIGRDA 
               
               
                   
               
               
                 AVQIYYSAFTNYLTSSSDFSNARDAVVQAAKDLYGASSAQATAAAKSFDA 
               
               
                   
               
               
                 VGVN 
               
            
           
         
       
     
     Example 1.2 
     Expression of  Paenibacillus  sp. Metalloprotease PspPro3 
     The DNA sequence of the propeptide-mature form of PspPro3 was synthesized and inserted into the  Bacillus subtilis  expression vector p2JM103BBI (Vogtentanz,  Protein Expr Purif,  55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX085 (AprE-PspPro3) ( FIG. 1.1 ). Ligation of the gene encoding the PspPro3 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3′ end of the  Bacillus subtilis  AprE signal sequence and the 5′ end of the predicted PspPro3 native propeptide. The gene has an alternative start codon (GTG). As shown in  FIG. 1.1 , pGX085(AprE-PspPro3) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in  B. subtilis , and the synthetic nucleotide sequence encoding the predicted propeptide and mature region of PspPro3 (SEQ ID NO: 4). The translation product of the synthetic AprE-PspPro3 gene is shown in SEQ ID NO: 5. 
       B. subtilis  cells (degU Hy  32, ΔscoC) were transformed with the pGX085(AprE-PspPro3) plasmid and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat #232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl 2 ). The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl 2  and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to a 150 mL Q Sepharose High Performance column pre-equilibrated with the loading buffer above and PspPro3 was then eluted from the column via the loading buffer supplemented with a linear NaCl gradient from 0 to 0.7 M. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses. 
     The nucleotide sequence of the synthesized PspPro3 gene in plasmid pGX085(AprE-PspPro3) is depicted in SEQ ID NO: 4. The sequence encoding the predicted native signal peptide is shown in italics and the region encoding the three residue addition (AGK) is shown in bold: 
     
       
         
           
               
            
               
                 
                   GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAA 
                 
               
               
                   
               
               
                   TCTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCT   GCTGGAAAAG C 
               
               
                   
               
               
                 AGAATCATCAGTGTCAGGACCGGCTCAGCTTACGCCGACGTTTCATGCA 
               
               
                   
               
               
                 GAGCAGTGGAAAGCACCGAGCAGCGTTAGCGGAGATGACATCGTGTGGA 
               
               
                   
               
               
                 GCTACCTGAACAGACAGAAGAAAACGCTTCTTGGCACGGACAGCACGAG 
               
               
                   
               
               
                 CGTCAGAGACCAGTTCAGAATCGTGGATAGAACAAGCGACAAAAGCGGC 
               
               
                   
               
               
                 GTCAGCCATTATAGACTGAAGCAGTATGTGAACGGAATCCCGGTTTATG 
               
               
                   
               
               
                 GCGCAGAACAAACAATCCATGTCGGAAAGAGCGGCGAAGTTACGAGCTA 
               
               
                   
               
               
                 TCTGGGCGCGGTTATTACAGAGGACCAGCAAGAGGAGGCTACACAAGGC 
               
               
                   
               
               
                 ACGACACCGAAAATTTCAGCATCAGAGGCAGTTCATACGGCCTACCAAG 
               
               
                   
               
               
                 AAGCTGCAACGAGAGTTCAAGCCCTGCCTACGTCAGATGATACAATCAG 
               
               
                   
               
               
                 CAAAGACGCTGAGGAACCTAGCTCAGTTAGCAAGGACACGTATAGCGAA 
               
               
                   
               
               
                 GCCGCGAACAATGGCAAGACGTCAAGCGTGGAAAAAGACAAGCTTTCAC 
               
               
                   
               
               
                 TGGAGAAGGCCGCTGATCTGAAAGACTCAAAGATCGAGGCTGTGGAAGC 
               
               
                   
               
               
                 GGAACCGAATAGCATTGCAAAGATTGCCAACCTGCAACCGGAGGTGGAC 
               
               
                   
               
               
                 CCGAAGGCGGAGCTGTATTTCTACGCTAAAGGCGATGCACTGCAACTGG 
               
               
                   
               
               
                 TTTACGTCACGGAGGTTAACATCCTGCAGCCGGCACCGCTTAGAACGAG 
               
               
                   
               
               
                 ATACATCATTGACGCGAACGACGGCAAGATCGTGAGCCAGTACGACATT 
               
               
                   
               
               
                 ATCAACGAGGCCACGGGAACGGGCAAGGGAGTCCTTGGCGACACGAAGA 
               
               
                   
               
               
                 CATTCAATACAACGGCCTCAGGCTCATCATACCAGCTGAGAGACACGAC 
               
               
                   
               
               
                 GAGAGGCAACGGAATCGTCACGTACACGGCTAGCAATAGACAGAGCATT 
               
               
                   
               
               
                 CCGGGCACAATCCTTACGGACGCAGACAATGTGTGGAATGACCCGGCAG 
               
               
                   
               
               
                 GCGTGGACGCACATGCCTACGCAGCGAAGACGTACGACTACTACAAGGA 
               
               
                   
               
               
                 GAAGTTCAACAGAAACAGCATCGACGGAAGAGGACTGCAACTTAGAAGC 
               
               
                   
               
               
                 ACGGTGCATTACGGCAACAGATACAACAACGCTTTCTGGAACGGCAGCC 
               
               
                   
               
               
                 AAATGACGTATGGAGACGGCGATGGAACAACGTTTATCGCATTCTCAGG 
               
               
                   
               
               
                 CGACCCTGACGTTGTGGGACATGAACTGACGCATGGAGTCACAGAATAC 
               
               
                   
               
               
                 ACGAGCAATCTGGAGTATTACGGAGAATCAGGCGCACTTAATGAGGCCT 
               
               
                   
               
               
                 TCAGCGACATCATCGGAAACGACATCCAGAGAAAGAACTGGCTGGTTGG 
               
               
                   
               
               
                 CGATGATATCTACACGCCGAGAATTGCGGGCGACGCGCTGAGATCAATG 
               
               
                   
               
               
                 AGCAACCCTACGCTGTACGATCAGCCGGATCATTACAGCAACCTGTATA 
               
               
                   
               
               
                 GAGGCTCAAGCGATAATGGCGGCGTGCATACAAACAGCGGCATCATCAA 
               
               
                   
               
               
                 CAAAGCCTATTATCTGCTGGCGCAAGGCGGCACATTCCATGGCGTTACA 
               
               
                   
               
               
                 GTTAATGGCATTGGCAGAGACGCAGCCGTGCAGATCTACTACAGCGCAT 
               
               
                   
               
               
                 TCACGAATTACCTGACATCAAGCAGCGACTTTTCAAATGCAAGAGATGC 
               
               
                   
               
               
                 AGTGGTGCAGGCGGCTAAAGACCTTTATGGAGCTTCAAGCGCTCAGGCC 
               
               
                   
               
               
                 ACAGCTGCGGCAAAAAGCTTCGACGCGGTTGGAGTGAAT 
               
            
           
         
       
     
     The amino acid sequence of the PspPro3 precursor protein expressed from plasmid pGX085(AprE-PspPro3) is depicted in SEQ ID NO: 5. The predicted signal sequence is shown in italics, the three residue addition (AGK) shown in bold and the predicted pro-peptide is shown in underlined text. 
     
       
         
           
               
            
               
                 
                   MRSKKLWISLLFALTIJFTMAFSNMSAQA 
                   AGK 
                   AESSVSGPAOLTPTFHA 
                 
               
               
                   
               
               
                 
                   EOWKAPSSVSGDDIVWSYLNROKKTLLGTDSTSVRDOFRIVDRTSDKSG 
                 
               
               
                   
               
               
                 
                   VSHYREKOYVNGIPVYGAEOTIHVGKSGEVTSYLGAVITEDOOEEATOG 
                 
               
               
                   
               
               
                 
                   TTPKISASEAVHTAYOEAATRVOALPTSDDTISKDAEEPSSVSKDTYSE 
                 
               
               
                   
               
               
                 
                   AANNGKTSSVEKDKLSLEKAADLKDSKIEAVEAEPNSIAKIANLOPEVD 
                 
               
               
                   
               
               
                 
                   PKAELYFYAKGDALOLVYVTEVNILOPAPLRTRYIIDANDGKIVSOYDI 
                 
               
               
                   
               
               
                   INE ATGTGKGVLGDTKTFNTTASGSSYQLRDTTRGNGIVTYTASNRQSI 
               
               
                   
               
               
                 PGTILTDADNVWNDPAGVDAHAYAAKTYDYYKEKFNRNSIDGRGLQLRS 
               
               
                   
               
               
                 TVHYGNRYNNAFWNGSQMTYGDGDGTTFIAFSGDPDVVGHELTHGVTEY 
               
               
                   
               
               
                 TSNLEYYGESGALNEAFSDIIGNDIQRKNWLVGDDIYTPRIAGDALRSM 
               
               
                   
               
               
                 SNPTLYDQPDHYSNLYRGSSDNGGVHTNSGIINKAYYLLAQGTFHGVTV 
               
               
                   
               
               
                 NGIGRDAAVQIYYSAFTNYLTSSSDFSNARDAVVQAAKDLYGASSAQAT 
               
               
                   
               
               
                 AAAKSFDAVGVN 
               
            
           
         
       
     
     The amino acid sequence of the PspPro3 recombinant protein isolated from  Bacillus subtilis  culture was determined by tandem mass spectrometry, and shown below. It is the same as predicted and depicted in SEQ ID NO: 3. 
     
       
         
           
               
            
               
                 ATGTGKGVLGDTKTFNTTASGSSYQLRDTTRGNGIVTYTASNRQSIPGTI 
               
               
                   
               
               
                 LTDADNVWNDPAGVDAHAYAAKTYDYYKEKFNRNSIDGRGLQLRSTVHYG 
               
               
                   
               
               
                 NRYNNAFWNGSQMTYGDGDGTTFIAFSGDPDVVGHELTHGVTEYTSNLEY 
               
               
                   
               
               
                 YGESGALNEAFSDIIGNDIQRKNWLVGDDIYTPRIAGDALRSMSNPTLYD 
               
               
                   
               
               
                 QPDHYSNLYRGSSDNGGVHTNSGIINKAYYLLAQGGTFHGVTVNGIGRDA 
               
               
                   
               
               
                 AVQIYYSAFTNYLTSSSDFSNARDAVVQAAKDLYGASSAQATAAAKSFDA 
               
               
                   
               
               
                 VGVN 
               
            
           
         
       
     
     Example 1.3 
     Proteolytic Activity of Metalloprotease PspPro3 
     The proteolytic activity of purified PspPro3 was measured in 50 mM Tris (pH 7), using azo-casein (Cat #74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μL of the diluted enzyme (or Milli-Q H 2 O alone as the blank control) was added to the non-binding 96-well microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μL of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μL of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μL supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A 440 ) using a SpectraMax 190. Net A 440  was calculated by subtracting the A 440  of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of duplicate assays, and the value varies no more than 5%. The proteolytic activity is shown as Net A 440 . The proteolytic assay with azo-casein as the substrate ( FIG. 1.2 ) indicates that PspPro3 is an active protease. 
     Example 1.4 
     pH Profile of Metalloprotease PspPro3 
     With azo-casein as the substrate, the pH profile of PspPro3 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μL of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μL diluted enzyme (250 ppm in Milli-Q H 2 O) in a 96-MTP placed on ice, followed by the addition of 48 μL of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 1.3. Enzyme activity at each pH was reported as relative activity where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in  FIG. 1.3 , the optimal pH of PspPro3 is 7.5, with greater than 70% of maximal activity retained between pH 5.5 and 9. 
     Example 1.5 
     Temperature Profile of Metalloprotease PspPro3 
     The temperature profiles of PspPro3 were analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assay. The enzyme sample and azo-casein substrate were prepared as in Example 3. Prior to the reaction, 50 μL of 1.5% azo-casein and 45 μl Milli-Q H 2 O were mixed in a 2000_, PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20-90° C.) for 5 min. After the incubation, 5 μL of diluted PspPro3 (100 ppm) or H 2 O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μL of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 1.3. The activity was reported as relative activity where the activity at the optimal temperature was set to be 100%. The tested temperatures were 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of triplicate assays. The data in  FIG. 1.4  suggest that PspPro3 showed an optimal temperature at 50° C., and retained greater than 70% of its maximal activity between 45° C. and 60° C. 
     Example 1.6 
     Cleaning Performance of Metalloprotease PspPro3 in Automatic Dishwashing (ADW) Conditions 
     The cleaning performance of PspPro3 in automatic dishwashing (ADW) conditions was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 or 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified PspPro3 were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl 2 , 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent (composition shown in Table 1.1) with 100 ppm water hardness (Ca 2+ :Mg 2+ =3:1), in the absence or presence of a bleach component (Peracid N,N-phthaloylaminoperoxycaproic acid-PAP). To initiate the reaction, 180 μL of AT detergent buffered at pH 6 or 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μL of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 1004, of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (A 405 ) (referred here as the “Initial performance”) using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μL water. Following the addition of 180 μL of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliter of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as “Wash-off”). The sum of two absorbance measurements (“Initial performance” plus “Wash-off”) gives the “Total performance”, which measures the protease activity on the model stain. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 for PspPro3 in AT detergent, in the absence or presence of bleach, is shown in  FIGS. 5A and 5B , respectively. 
     
       
         
           
               
             
               
                 TABLE 1.1 
               
             
            
               
                   
               
               
                 Composition of AT dish detergent formula with bleach 
               
            
           
           
               
               
            
               
                 Ingredient 
                 Concentration (mg/ml) 
               
               
                   
               
               
                 MGDA (methylglycinediacetic acid) 
                 0.143 
               
               
                 Sodium citrate 
                 1.86  
               
               
                 Citric acid* 
                 varies 
               
               
                 PAP (peracid N, N-phthaloylaminoperoxycaproic acid) 
                 0.057 
               
               
                 Plurafac ® LF 18B (a non-ionic surfactant) 
                 0.029 
               
               
                 Bismuthcitrate 
                 0.006 
               
               
                 Bayhibit ® S (Phosphonobutantricarboxylic acid sodium salt) 
                 0.006 
               
               
                 Acusol ™ 587 (a calcium polyphosphate inhibitor) 
                 0.029 
               
               
                 PEG 6000 
                 0.043 
               
               
                 PEG 1500 
                 0.1  
               
               
                   
               
               
                 *The pH of the AT detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9 M citric acid. 
               
            
           
         
       
     
     Example 1.7 
     Cleaning Performance of Metalloprotease PspPro3 in Laundry Conditions 
     The cleaning performance of PspPro3 protein in liquid laundry detergent was tested using EMPA-116 (cotton soiled with blood/milk/ink) microswatches (obtained from CFT Vlaardingen, The Netherlands) at pH 8.2 using a commercial detergent. Prior to the reaction, purified PspPro3 protein samples were diluted with a dilution solution (10 mM NaCl, 0.1 mM CaCl 2 , 0.005% TWEEN® 80 and 10% propylene glycol) to the desired concentrations; and the commercial detergent (Tide®, Clean Breeze®, Proctor &amp; Gamble, USA, purchased September 2011) was incubated at 95° C. for 1 hour to inactivate the enzymes present in the detergent. Proteolytic assays were subsequently performed to confirm the inactivation of proteases in the commercial detergent. The heat treated detergent was further diluted with 5 mM HEPES (pH 8.2) to a final concentration of 0.788 g/L. Meanwhile, the water hardness of the buffered liquid detergent was adjusted to 103 ppm (Ca 2+ :Mg 2+ =3:1). The specific conductivity of the buffered detergent was adjusted to either 0.62 mS/cm (low conductivity) or 3.5 mS/cm (high conductivity) by adjusting the NaCl concentration in the buffered detergent. To initiate the reaction, 190 μl of either the high or low conductivity buffered detergent was added to a 96-MTP containing the EMPA-116 microswatches, followed by the addition of 10 μl of diluted enzyme (or the dilution solution as blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 20 min at 32° C. and 1150 rpm. After incubation, 150 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 600 nm using a spectrophotometer, which indicates the protease activity on the model stain; and Net A 600  was subsequently calculated by subtracting the A 600  of the blank control from that of the enzyme. Dose response for the cleaning of EMPA-116 microswatches in liquid laundry detergent at high or low conductivity is shown in  FIG. 1.6 . 
     Example 1.8 
     Comparison of PspPro3 to Other Metalloproteases 
     A. Identification of Homologous Proteases 
     Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The mature protein amino acid sequence for PspPro3 (SEQ ID NO: 3) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 1.2A and 1.2B provide a list of sequences with the percent identity to PspPro3. The length in Table 1.2 refers to the entire sequence length of the homologous proteases. 
     
       
         
           
               
             
               
                 TABLE 1.2A 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PspPro3 protein identified from 
               
               
                 the NCBI non-redundant protein database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Accession # 
                 PspPro3 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 ZP_10321515.1 
                  55 
                   Bacillus macauensis  ZFHKF-1 
                 552 
               
               
                 AAC43402.1 
                  57 
                 
                   Alicyclobacillus acidocaldarius 
                 
                 546 
               
               
                 P00800 
                  57 
                 
                   Bacillus thermoproteolyticus 
                 
                 548 
               
               
                 AAA22621.1 
                  58 
                 
                   Geobacillus stearothermophilus 
                 
                 548 
               
               
                 ZP_01862236.1 
                  59 
                   Bacillus  sp. SG-1 
                 560 
               
               
                 YP_002884504.1 
                  59 
                   Exiguobacterium  sp. AT1b 
                 509 
               
               
                 AEI46285.1 
                  60 
                   Paenibacillus mucilaginosus  KNP414 
                 525 
               
               
                 ZP_08093424 
                  60 
                   Planococcus donghaensis  MPA1U2 
                 553 
               
               
                 ZP_10324092.1 
                  61 
                   Bacillus macauensis  ZFHKF-1 
                 533 
               
               
                 YP_006792441.1 
                  61 
                   Exiguobacterium antarcticum  B7 
                 498 
               
               
                 AAK69076.1 
                  63 
                 
                   Bacillus thuringiensis serovar finitimus 
                 
                 566 
               
               
                 NP_976992.1 
                  64 
                   Bacillus cereus  ATCC 10987 
                 566 
               
               
                 ZP_04321694 
                  64 
                 
                   Bacillus cereus 
                 
                 566 
               
               
                 BAA06144 
                  64 
                   Lactobacillus  sp. 
                 566 
               
               
                 ZP_10241029.1 
                  78 
                   Paenibacillus peoriae  KCTC 3763 
                 599 
               
               
                 YP_005073223 
                  93 
                   Paenibacillus terrae  HPL-003 
                 591 
               
               
                 YP_003872179 
                  94 
                   Paenibacillus polymyxa  E681 
                 592 
               
               
                 ZP_09775364 
                 100 
                   Paenibacillus  sp. Aloe-11 
                 593 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 1.2B 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PspPro3 protein identified 
               
               
                 from the Genome Quest Patent database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Patent # 
                 PspPro3 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 US20120107907-0184 
                 57.88 
                 
                   Bacillus caldoyticus 
                 
                 319 
               
               
                 US20120107907-0177 
                 57.88 
                 
                   Bacillus caldolyticus 
                 
                 544 
               
               
                 WO2012110563-0002 
                 58.2  
                 
                   Bacillus caldolyticus 
                 
                 319 
               
               
                 EP2390321-0176 
                 58.52 
                 
                   Bacillus stearothermophilis 
                 
                 548 
               
               
                 US6518054-0002 
                 59.22 
                   Bacillus  sp .   
                 316 
               
               
                 WO2004011619-0044 
                 60.6  
                 Empty 
                 507 
               
               
                 WO2004011619-0047 
                 62.14 
                 Empty 
                 532 
               
               
                 WO2004011619-0046 
                 62.26 
                 Empty 
                 536 
               
               
                 WO2012110563-0004 
                 63.02 
                 
                   Bacillus megaterium 
                 
                 320 
               
               
                 JP2002272453-0003 
                 63.67 
                 Empty 
                 562 
               
               
                 US8114656-0186 
                 64.24 
                 
                   Bacillus brevis 
                 
                 304 
               
               
                 WO2012110562-0005 
                 64.52 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 WO2007044993-0178 
                 64.74 
                 
                   Bacillus thuringiensis 
                 
                 566 
               
               
                 EP2178896-0184 
                 65.38 
                 
                   Bacillus anthracis 
                 
                 566 
               
               
                 WO2012110563-0005 
                 65.48 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 JP1995184649-0001 
                 65.71 
                   Lactobacillus  sp. 
                 566 
               
               
                 US5962264-0004 
                 65.81 
                 Empty 
                 566 
               
               
                 US20120107907-0185 
                 66.13 
                 
                   Bacillus cereus 
                 
                 317 
               
               
                 US8114656-0187 
                 93.36 
                 
                   Bacillus polymyxa 
                 
                 302 
               
               
                 JP2005229807-0019 
                 93.38 
                 
                   Paenibacillus polymyxa 
                 
                 566 
               
               
                   
               
            
           
         
       
     
     B. Alignment of Homologous Protease Sequences 
     The amino acid sequence for mature PspPro3 (SEQ ID NO: 3) was aligned with thermolysin (P00800,  Bacillus thermoproteolyticus ) and protease from  Paenibacillus  sp. Aloe-11 (ZP_09775364) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters.  FIG. 1.7  shows the alignment of PspPro3 with these protease sequences. 
     C. Phylogenetic Tree 
     A phylogenetic tree for full length sequence of PspPro3 (SEQ ID NO: 2) was built using sequences of representative homologs from Tables 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. MolBiol.Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in  FIG. 1.8 . 
     Example 1.9 
     Terg-o-Tometer Performance Evaluation of PspPro3 
     The wash performance of PspPro3 was tested in a laundry detergent application using a Terg-o-Tometer (Instrument Marketing Services, Inc, Fairfield, N.J.). The performance evaluation was conducted at 32° C. and 16° C. The soil load consisted of two of each of the following stain swatches: EMPA116 Blood, Milk, Ink on cotton (Test materials AG, St. Gallen, Switzerland), EMPA117 Blood, Milk, Ink on polycotton (Test materials AG, St. Gallen, Switzerland), EMPA112 Cocoa on cotton (Test materials AG, St. Gallen, Switzerland), and CFT C-10 Pigment, Oil, and Milk content on cotton (Center for Testmaterials BV, Vlaardingen, Netherlands), plus extra white interlock knit fabric to bring the total fabric load to 40 g per beaker of the Terg-o-Tometer, which was filled with 1 L of deionized water. The water hardness was adjusted to 6 grains per gallon, and the pH in the beaker was buffered with 5 mM HEPES, pH 8.2. Heat inactivated Tide Regular HDL (Procter &amp; Gamble), a commercial liquid detergent purchased in a local US supermarket, was used at 0.8 g/L. The detergent was inactivated before use by treatment at 92° C. in a water bath for 2-3 hours followed by cooling to room temperature. Heat inactivation of commercial detergents serves to destroy the activity of enzymatic components while retaining the properties of the non-enzymatic components. Enzyme activity in the heat inactivated detergent was measured using the Suc-AAPF-pNA assay for measuring protease activity. The Purafect® Prime HA, (Genencor Int&#39;l) and PspPro3 proteases were each added to final concentrations of 0, 0.2, 0.5, 1, and 1.5 ppm. The wash time was 12 minutes. After the wash treatment, all swatches were rinsed for 3 minutes and machine-dried at low heat. 
     Four of each types of swatch were measured before and after treatment by optical reflectance using a Tristimulus Minolta Meter CR-400. The difference in the L, a, b values was converted to total color difference (dE), as defined by the CIE-LAB color space. Cleaning of the stains is expressed as percent stain removal index (% SRI) by taking a ratio between the color difference before and after washing, and comparing it to the difference of unwashed soils (before wash) to unsoiled fabric, and averaging the eight values obtained by reading two different regions of each washed swatch and is reported in Tables 1.9A and 1.9B as Average % SRI (dE)±95CI. Table 1.9A summarizes the cleaning performance of PspPro3 at 32° C. and Table 1.9B at 16° C. 
     
       
         
           
               
             
               
                 TABLE 1.9A 
               
               
                   
               
               
                 Cleaning performance of PspPro3 at 32° C. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 EMPA-116 
                 EMPA-117 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Purafect Prime 
                   
                 Purafect Prime 
                   
               
               
                   
                 HA 
                 PspPro3 
                 HA 
                 SprPro3 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
               
               
                 ppm 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
               
               
                 enzyme 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
               
               
                   
               
               
                 0 
                 0.19 
                 0.01 
                 0.19 
                 0.01 
                 0.17 
                 0.01 
                 0.17 
                 0.01 
               
               
                 0.2 
                 0.27 
                 0.02 
                 0.27 
                 0.02 
                 0.25 
                 0.03 
                 0.30 
                 0.02 
               
               
                 0.5 
                 0.28 
                 0.03 
                 0.31 
                 0.01 
                 0.30 
                 0.03 
                 0.31 
                 0.02 
               
               
                 1 
                 0.30 
                 0.01 
                 0.32 
                 0.02 
                 0.35 
                 0.02 
                 0.34 
                 0.03 
               
               
                 1.5 
                 0.31 
                 0.02 
                 0.31 
                 0.01 
                 0.37 
                 0.01 
                 0.37 
                 0.03 
               
               
                   
               
            
           
           
               
               
               
            
               
                   
                 EMPA-112 
                 CFT C-10 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Purafect Prime 
                   
                 Purafect Prime 
                   
               
               
                   
                 HA 
                 PspPro3 
                 HA 
                 PspPro3 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
               
               
                 ppm 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
               
               
                 enzyme 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
               
               
                   
               
               
                 0 
                 0.11 
                 0.03 
                 0.11 
                 0.03 
                 0.07 
                 0.01 
                 0.07 
                 0.01 
               
               
                 0.2 
                 0.11 
                 0.05 
                 0.18 
                 0.04 
                 0.12 
                 0.01 
                 0.11 
                 0.01 
               
               
                 0.5 
                 0.13 
                 0.04 
                 0.17 
                 0.03 
                 0.15 
                 0.01 
                 0.16 
                 0.01 
               
               
                 1 
                 0.18 
                 0.03 
                 0.19 
                 0.04 
                 0.17 
                 0.01 
                 0.21 
                 0.01 
               
               
                 1.5 
                 0.19 
                 0.03 
                 0.18 
                 0.04 
                 0.18 
                 0.01 
                 0.23 
                 0.01 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 1.9B 
               
               
                   
               
               
                 Cleaning performance of PspPro3 at 16° C. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 EMPA-116 
                 EMPA-117 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Purafect Prime 
                   
                 Purafect Prime 
                   
               
               
                   
                 HA 
                 PspPro3 
                 HA 
                 PspPro3 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
               
               
                 ppm 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
               
               
                 enzyme 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
               
               
                   
               
               
                 0 
                 0.15 
                 0.02 
                 0.15 
                 0.02 
                 0 13 
                 0.01 
                 0 13 
                 0.01 
               
               
                 0.2 
                 0.19 
                 0.02 
                 0.20 
                 0.03 
                 0.15 
                 0.02 
                 0.15 
                 0.02 
               
               
                 0.5 
                 0.20 
                 0.02 
                 0.19 
                 0.02 
                 0.21 
                 0.02 
                 0.20 
                 0.02 
               
               
                 1 
                 0.24 
                 0.04 
                 0.21 
                 0.02 
                 0.22 
                 0.02 
                 0.20 
                 0.01 
               
               
                 1.5 
                 0.19 
                 0.02 
                 0.25 
                 0.04 
                 0.23 
                 0.03 
                 0.20 
                 0.01 
               
               
                   
               
            
           
           
               
               
               
            
               
                   
                 EMPA-112 
                 CFT C-10 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Purafect Prime 
                   
                 Purafect Prime 
                   
               
               
                   
                 HA 
                 PspPro3 
                 HA 
                 PspPro3 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
               
               
                 ppm 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
               
               
                 enzyme 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
               
               
                   
               
               
                 0 
                 0.08 
                 0.03 
                 0.08 
                 0.03 
                 0.04 
                 0.08 
                 0.04 
                 0.08 
               
               
                 0.2 
                 0.12 
                 0.02 
                 0.09 
                 0.01 
                 0.06 
                 0.12 
                 0.06 
                 0.09 
               
               
                 0.5 
                 0.08 
                 0.02 
                 0.11 
                 0.02 
                 0.08 
                 0.08 
                 0.08 
                 0.11 
               
               
                 1 
                 0.11 
                 0.02 
                 0.10 
                 0.03 
                 0.08 
                 0.11 
                 0.09 
                 0.10 
               
               
                 1.5 
                 0.13 
                 0.02 
                 0.11 
                 0.03 
                 0.11 
                 0.13 
                 0.10 
                 0.11 
               
               
                   
               
            
           
         
       
     
     Example 2.1 
     Cloning of Metalloprotease PspPro2 from  Paenibacillus  sp. 
     A strain of  Paenibacillus  sp. was selected as a potential source for enzymes which may be useful for various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the  Paenibacillus  sp. strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina&#39;s next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in  Paenibacillus  sp. encodes a metalloprotease and the sequence of this gene, called PspPro2, is provided in SEQ ID NO: 6. The corresponding protein encoded by the PspPro2 gene is shown in SEQ ID NO: 7. At the N-terminus, the protein has a signal peptide with a length of 24 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PspPro2 is a secreted enzyme. The propeptide region of PspPro2 was predicted based on protein sequence alignment with the  Paenibacillus polymyxa  Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PspPro2 is shown in SEQ ID NO: 8. 
     The nucleotide sequence of the PspPro2 gene isolated from  Paenibacillus  sp. is set forth as SEQ ID NO: 6. The sequence encoding the predicted native signal peptide is shown in italics: 
     
       
         
           
               
            
               
                 
                   ATGAAAAAAGTATGGGTTTCACTTCTTGGAGGAGCGATGTTATTAGGGG 
                 
               
               
                   
               
               
                   CTGTAGCACCAGGTGCATCAGCA GCAGAGCATTCTGTTCCTGATCCTAC 
               
               
                   
               
               
                 TCAGCTAACACCGACCTTTCACGCCGAGCAATGGAAGGCTCCTTCCACG 
               
               
                   
               
               
                 GTAACCGGCGACAATATTGTATGGAGCTATTTGAATCGACAAAAGAAAA 
               
               
                   
               
               
                 CCTTATTGAATACAGACAGCACCAGTGTGCGTGATCAGTTCCGCATCAT 
               
               
                   
               
               
                 TGATCGTACAAGCGACAAATCCGGTGCAAGCCATTATCGGCTCAAGCAA 
               
               
                   
               
               
                 TATGTAAACGGGATCCCCGTATATGGGGCTGAACAGACCATTCATGTGA 
               
               
                   
               
               
                 ACAACGCCGGTAAAGTAACCTCTTATTTGGGTGCTGTCATTTCAGAGGA 
               
               
                   
               
               
                 TCAGCAGCAAGACGCGACCGAAGATACCACTCCAAAAATCAGCGCGACT 
               
               
                   
               
               
                 GAAGCCGTTTATACCGCATATGCAGAAGCCGCTGCCCGGATTCAATCCT 
               
               
                   
               
               
                 TCCCTTCCATCAATGATAGTCTTTCTGAGGCTAGTGAGGAACAAGGGAG 
               
               
                   
               
               
                 TGAGAATCAAGGCAATGAGATTCAAAACATTGGGATTAAAAGCAGTGTA 
               
               
                   
               
               
                 AGTAATGACACTTACGCAGAGGCGCATAACAACGTACTTTTAACCCCCG 
               
               
                   
               
               
                 TTGACCAAGCAGAGCAAAGTTACATTGCCAAAATTGCTAATCTGGAGCC 
               
               
                   
               
               
                 AAGTGTAGAGCCCAAAGCAGAATTATACATCTATCCAGATGGTGAGACT 
               
               
                   
               
               
                 ACACGACTGGTTTATGTAACAGAGGTTAATATTCTTGAACCTGCGCCTC 
               
               
                   
               
               
                 TGCGCACACGCTACTTCATTGATGCGAAAACCGGCAAAATCGTATTCCA 
               
               
                   
               
               
                 GTATGACATCCTCAACCACGCAACAGGCACCGGCCGCGGCGTGGATGGC 
               
               
                   
               
               
                 AAAACAAAATCATTTACGACTACAGCTTCAGGCAACCGGTATCAGTTGA 
               
               
                   
               
               
                 AAGACACGACTCGCAGCAATGGAATCGTGACTTACACCGCTGGCAATCG 
               
               
                   
               
               
                 CCAGACGACGCCAGGTACGATTTTGACCGATACAGATAATGTATGGGAG 
               
               
                   
               
               
                 GACCCTGCGGCTGTTGATGCCCATGCCTACGCCATTAAAACCTATGACT 
               
               
                   
               
               
                 ATTATAAGAATAAATTCGGTCGCGACAGTATTGATGGACGTGGCATGCA 
               
               
                   
               
               
                 AATTCGTTCGACAGTCCATTACGGCAAAAAATATAACAATGCCTTCTGG 
               
               
                   
               
               
                 AACGGCTCGCAAATGACCTACGGAGACGGAGACGGGTCCACATTTACCT 
               
               
                   
               
               
                 TCTTCAGCGGCGATCCCGATGTCGTGGGGCATGAGCTCACCCACGGCGT 
               
               
                   
               
               
                 CACCGAGTTCACCTCCAATTTGGAGTATTATGGTGAGTCCGGTGCATTG 
               
               
                   
               
               
                 AACGAAGCCTTCTCGGATATTATCGGTAATGATATAGATGGCACCAGTT 
               
               
                   
               
               
                 GGCTTCTTGGCGACGGCATTTATACGCCTAATATTCCAGGCGACGCTCT 
               
               
                   
               
               
                 GCGTTCCCTGTCCGATCCTACACGATTCGGCCAGCCGGATCACTACTCC 
               
               
                   
               
               
                 AATTTCTATCCGGACCCCAACAATGATGATGAAGGCGGAGTCCATACGA 
               
               
                   
               
               
                 ACAGCGGTATTATCAACAAAGCCTATTATTTGCTGGCACAAGGCGGTAC 
               
               
                   
               
               
                 GTCCCATGGTGTAACGGTAACTGGTATCGGACGCGAAGCGGCTGTATTC 
               
               
                   
               
               
                 ATTTACTACAATGCCTTTACCAACTATTTGACCTCTACCTCCAACTTCT 
               
               
                   
               
               
                 CTAACGCACGCGCTGCTGTTATACAGGCAGCCAAGGATTTTTATGGTGC 
               
               
                   
               
               
                 TGATTCGCTGGCAGTAACCAGTGCTATTCAATCCTTTGATGCGGTAGGA 
               
               
                   
               
               
                 ATCAAA 
               
            
           
         
       
     
     The amino acid sequence of the PspPro2 precursor protein is set forth as SEQ ID NO: 7. The predicted signal peptide is shown in italics, and the predicted pro-peptide is shown in underlined text: 
     
       
         
           
               
            
               
                 M KKVWVSLLGGAMLLGAVAPGASA   AEHSVPDPTQLTPTFHAEQWKAPSTV   
               
               
                   
               
               
                 
                   TGDNIVWSYLNRQKKTLLNTDSTSVRDQFRIIDRTSDKSGASHYRLKQYV 
                 
               
               
                   
               
               
                 
                   NGIPVYGAEQTIHVNNAGKVTSYLGAVISEDQQQDATEDTTPKISATEAV 
                 
               
               
                   
               
               
                 
                   YTAYAEAAARIQSFPSINDSLSEASEEQGSENQGNEIQNIGIKSSVSNDT 
                 
               
               
                   
               
               
                 
                   YAEAHNNVLLTPVDQAEQSYIAKIANLEPSVEPKAELYIYPDGETTRLVY 
                 
               
               
                   
               
               
                   VTEVNILEPAPLRTRYFIDAKTGKIVFQYDILNH ATGTGRGVDGKTKSFT 
               
               
                   
               
               
                 TTASGNRYQLKDTTRSNGIVTYTAGNRQTTPGTILTDTDNVWEDPAAVDA 
               
               
                   
               
               
                 HAYAIKTYDYYKNKFGRDSIDGRGMQIRSTVHYGKKYNNAFWNGSQMTYG 
               
               
                   
               
               
                 DGDGSTFTFFSGDPDVVGHELTHGVTEFTSNLEYYGESGALNEAFSDIIG 
               
               
                   
               
               
                 NDIDGTSWLLGDGIYTPNIPGDALRSLSDPTRFGQPDHYSNFYPDPNNDD 
               
               
                   
               
               
                 EGGVHTNSGIINKAYYLLAQGGTSHGVTVTGIGREAAVFIYYNAFTNYLT 
               
               
                   
               
               
                 STSNFSNARAAVIQAAKDFYGADSLAVTSAIQSFDAVGIK 
               
            
           
         
       
     
     The amino acid sequence of the predicted mature form of PspPro2 is set forth as SEQ ID NO: 8. 
     
       
         
           
               
            
               
                 ATGTGRGVDGKTKSFTTTASGNRYQLKDTTRSNGIVTYTAGNRQTTPGTI 
               
               
                   
               
               
                 LTDTDNVWEDPAAVDAHAYAIKTYDYYKNKFGRDSIDGRGMQIRSTVHYG 
               
               
                   
               
               
                 KKYNNAFWNGSQMTYGDGDGSTFTFFSGDPDVVGHELTHGVTEFTSNLEY 
               
               
                   
               
               
                 YGESGALNEAFSDIIGNDIDGTSWLLGDGIYTPNIPGDALRSLSDPTRFG 
               
               
                   
               
               
                 QPDHYSNFYPDPNNDDEGGVHTNSGIINKAYYLLAQGGTSHGVTVTGIGR 
               
               
                   
               
               
                 EAAVFIYYNAFTNYLTSTSNFSNARAAVIQAAKDFYGADSLAVTSAIQSF 
               
               
                   
               
               
                 DAVGIK 
               
            
           
         
       
     
     Example 2.2 
     Expression of  Paenibacillus  sp. Metalloprotease PspPro2 
     The DNA sequence of the propeptide-mature form of PspPro2 was synthesized and inserted into the  Bacillus subtilis  expression vector p2JM103BBI (Vogtentanz,  Protein Expr Purif,  55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX084 (AprE-PspPro2) ( FIG. 2.1 ). Ligation of this gene encoding the PspPro2 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3′ end of the  Bacillus subtilis  AprE signal sequence and the 5′ end of the predicted PspPro2 native propeptide. The gene has an alternative start codon (GTG). As shown in  FIG. 2.1 , pGX084(AprE-PspPro2) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in  B. subtilis , and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PspPro2, (SEQ ID NO: 9). The translation product of the synthetic AprE-PspPro2 gene is shown in SEQ ID NO: 10. 
     The pGX084(AprE-PspPro2) plasmid was transformed into  B. subtilis  cells (degU Hy  32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm chloramphenicol and 1.2% skim milk (Cat #232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl 2 ). 
     The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5) and 1 mM CaCl 2  using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to a 150 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above, PspPro2 was eluted from the column with a linear salt gradient from 0 to 0.5 M NaCl in the loading buffer. The corresponding active fractions were collected, concentrated and buffer-exchanged again into the loading buffer described above. The sample was loaded onto a 20 ml DEAE Fast Flow column pre-equilibrated with the same loading buffer. PspPro2 was eluted from the column with a linear salt gradient from 0 to 0.3 M NaCl in the loading buffer. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses. The nucleotide sequence of the synthesized PspPro2 gene in plasmid pGX084 (AprE-PspPro2) is depicted in SEQ ID NO: 9. The sequence encoding the predicted native signal peptide is shown in italics and the oligo-nucleotide encoding the three residue addition (AGK) is shown in bold: 
     
       
         
           
               
            
               
                 
                   GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAA 
                 
               
               
                   
               
               
                   TCTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCT   GCTGGAAAA GC 
               
               
                   
               
               
                 AGAGCATTCAGTTCCTGACCCGACGCAACTTACACCGACATTTCATGCT 
               
               
                   
               
               
                 GAGCAGTGGAAGGCACCGAGCACGGTCACGGGCGACAACATCGTGTGGA 
               
               
                   
               
               
                 GCTACCTGAACAGACAGAAAAAGACGCTGCTGAACACGGACTCAACGAG 
               
               
                   
               
               
                 CGTGAGAGACCAGTTCAGAATCATCGACAGAACGAGCGACAAGTCAGGC 
               
               
                   
               
               
                 GCGTCACATTATAGACTGAAGCAGTACGTGAACGGCATCCCGGTCTACG 
               
               
                   
               
               
                 GAGCCGAGCAAACGATCCATGTGAATAATGCGGGCAAAGTTACATCATA 
               
               
                   
               
               
                 CCTGGGCGCCGTCATCTCAGAAGACCAGCAGCAAGATGCAACGGAGGAT 
               
               
                   
               
               
                 ACAACACCGAAGATCAGCGCCACAGAAGCGGTCTATACGGCTTACGCCG 
               
               
                   
               
               
                 AAGCGGCTGCAAGAATCCAGAGCTTCCCGTCAATTAATGACAGCCTGAG 
               
               
                   
               
               
                 CGAAGCATCAGAGGAACAAGGCAGCGAGAACCAGGGCAATGAAATCCAA 
               
               
                   
               
               
                 AACATCGGCATCAAGAGCAGCGTGTCAAACGACACGTATGCGGAGGCTC 
               
               
                   
               
               
                 ATAACAACGTTCTGCTGACACCGGTCGATCAGGCCGAACAGAGCTATAT 
               
               
                   
               
               
                 TGCAAAGATCGCGAATCTGGAGCCGTCAGTCGAGCCGAAGGCCGAGCTG 
               
               
                   
               
               
                 TATATCTATCCGGACGGCGAGACGACGAGACTGGTGTACGTTACGGAGG 
               
               
                   
               
               
                 TCAACATCCTTGAGCCTGCGCCGCTGAGAACAAGATACTTTATCGACGC 
               
               
                   
               
               
                 CAAGACGGGCAAGATCGTGTTTCAGTACGATATCCTGAACCATGCGACG 
               
               
                   
               
               
                 GGAACAGGCAGAGGCGTGGACGGCAAAACAAAATCATTCACGACAACGG 
               
               
                   
               
               
                 CAAGCGGCAACAGATACCAGCTGAAGGACACAACAAGATCAAATGGCAT 
               
               
                   
               
               
                 CGTCACATACACGGCCGGAAATAGACAGACGACGCCGGGAACGATTCTG 
               
               
                   
               
               
                 ACGGATACAGATAACGTGTGGGAAGATCCGGCAGCAGTTGATGCACATG 
               
               
                   
               
               
                 CATACGCGATCAAGACGTACGACTACTACAAGAACAAATTCGGAAGAGA 
               
               
                   
               
               
                 TTCAATCGATGGAAGAGGCATGCAAATCAGATCAACGGTTCATTATGGC 
               
               
                   
               
               
                 AAAAAGTACAACAATGCCTTCTGGAACGGCAGCCAAATGACATACGGCG 
               
               
                   
               
               
                 ATGGAGACGGCTCAACGTTTACATTCTTTTCAGGCGACCCGGACGTCGT 
               
               
                   
               
               
                 CGGCCATGAACTGACGCATGGCGTTACAGAGTTCACGAGCAACCTGGAG 
               
               
                   
               
               
                 TATTACGGCGAATCAGGCGCACTGAATGAGGCTTTCAGCGACATCATTG 
               
               
                   
               
               
                 GCAACGACATTGATGGCACATCATGGCTGCTTGGCGACGGCATTTACAC 
               
               
                   
               
               
                 ACCTAACATTCCGGGCGATGCACTGAGAAGCCTGTCAGACCCTACGAGA 
               
               
                   
               
               
                 TTCGGCCAACCTGACCATTACAGCAACTTCTACCCGGATCCTAATAACG 
               
               
                   
               
               
                 ATGATGAGGGCGGAGTGCATACGAACAGCGGCATTATCAACAAAGCGTA 
               
               
                   
               
               
                 CTATCTGCTGGCACAAGGCGGAACGTCACATGGAGTGACGGTGACAGGA 
               
               
                   
               
               
                 ATCGGCAGAGAGGCGGCAGTGTTTATCTACTACAACGCCTTCACAAACT 
               
               
                   
               
               
                 ACCTGACGAGCACGTCAAATTTCAGCAACGCTAGAGCGGCGGTCATCCA 
               
               
                   
               
               
                 GGCAGCAAAGGACTTTTATGGAGCAGACTCACTGGCAGTTACGTCAGCA 
               
               
                   
               
               
                 ATTCAGTCATTCGACGCAGTTGGAATTAAG 
               
            
           
         
       
     
     The amino acid sequence of the PspPro2 precursor protein expressed from plasmid pGX084(AprE-PspPro2) is depicted in SEQ ID NO: 10. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text: 
     
       
         
           
               
            
               
                 
                   MRSKKLWISLLFALTLIFTMAFS1VMSAQA 
                   AGK 
                   AEHSVPDPTQLTPTFH 
                 
               
               
                   
               
               
                 
                   AEQWKAPSTVTGDNIVWSYLNRQKKTLLNTDSTSVRDQFRIIDRTSDKS 
                 
               
               
                   
               
               
                 
                   GASHYRLKQYVNGIPVYGAEQTIHVNNAGKVTSYLGAVISEDQQQDATE 
                 
               
               
                   
               
               
                 
                   DTTPKISATEAVYTAYAEAAARIQSFPSINDSLSEASEEQGSENQGNEI 
                 
               
               
                   
               
               
                 
                   QNIGIKSSVSNDTYAEAHNNVLLTPVDQAEQSYIAKIANLEPSVEPKAE 
                 
               
               
                   
               
               
                   LYIYPDGETTRLVYVTEVNILEPAPLRTRYFIDAKTGKIVFQYDILNH A 
               
               
                   
               
               
                 TGTGRGVDGKTKSFTTTASGNRYQLKDTTRSNGIVTYTAGNRQTTPGTI 
               
               
                   
               
               
                 LTDTDNVWEDPAAVDAHAYAIKTYDYYKNKFGRDSIDGRGMQIRSTVHY 
               
               
                   
               
               
                 GKKYNNAFWNGSQMTYGDGDGSTFTFFSGDPDVVGHELTHGVTEFTSNL 
               
               
                   
               
               
                 EYYGESGALNEAFSDIIGNDIDGTSWLLGDGIYTPNIPGDALRSLSDPT 
               
               
                   
               
               
                 RFGQPDHYSNFYPDPNNDDEGGVHTNSGIINKAYYLLAQGGTSHGVTVT 
               
               
                   
               
               
                 GIGREAAVFIYYNAFTNYLTSTSNFSNARAAVIQAAKDFYGADSLAVTS 
               
               
                   
               
               
                 AIQSFDAVGIK 
               
            
           
         
       
     
     The amino acid sequence of the recombinant PspPro2 protein isolated from  Bacillus subtilis  culture was determined by tandem mass spectrometry, and shown below. It is the same as predicted and depicted in SEQ ID NO: 8. 
     
       
         
           
               
            
               
                 ATGTGRGVDGKTKSFTTTASGNRYQLKDTTRSNGIVTYTAGNRQTTPGTI 
               
               
                   
               
               
                 LTDTDNVWEDPAAVDAHAYAIKTYDYYKNKFGRDSIDGRGMQIRSTVHYG 
               
               
                   
               
               
                 KKYNNAFWNGSQMTYGDGDGSTFTFFSGDPDVVGHELTHGVTEFTSNLEY 
               
               
                   
               
               
                 YGESGALNEAFSDIIGNDIDGTSWLLGDGIYTPNIPGDALRSLSDPTRFG 
               
               
                   
               
               
                 QPDHYSNFYPDPNNDDEGGVHTNSGIINKAYYLLAQGGTSHGVTVTGIGR 
               
               
                   
               
               
                 EAAVFIYYNAFTNYLTSTSNFSNARAAVIQAAKDFYGADSLAVTSAIQSF 
               
               
                   
               
               
                 DAVGIK 
               
            
           
         
       
     
     Example 2.3 
     Proteolytic Activity of Metalloprotease PspPro2 
     The proteolytic activity of purified PspPro2 was measured in 50 mM Tris (pH 7), using azo-casein (Cat #74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H 2 O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A 440 ) using a SpectraMax 190. Net A 440  was calculated by subtracting the A 440  of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of duplicate assays, and the value varies no more than 5%. The proteolytic activity is shown as Net A 440 . The proteolytic assays with azo-casein as the substrate ( FIG. 2.2 ) indicate that PspPro2 is an active protease. 
     Example 2.4 
     pH Profile of Metalloprotease PspPro2 
     With azo-casein as the substrate, the pH profile of PspPro2 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl diluted enzyme (500 ppm in Milli-Q H 2 O) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 2.3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10 and 11. Each result was the mean of triplicate assays. As shown in  FIG. 2.3 , the optimal pH of PspPro2 is 7.5 with greater than 70% of its maximal activity retained between pH 5.5 and 9.5. 
     Example 2.5 
     Temperature Profile of Metalloprotease PspPro2 
     The temperature profile of PspPro2 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assay. The enzyme sample and azo-casein substrate were prepared as in Example 2.3. 
     Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H 2 O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20-90° C.) for 5 min. After the incubation, 5 μl of diluted PspPro2 (200 ppm) or H 2 O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80 and 90° C. Each result was the mean of triplicate assays. The data in  FIG. 2.4  suggest that PspPro2 showed an optimal temperature at 50° C., and retained greater than 70% of its maximal activity between 40 and 65° C. 
     Example 2.6 
     Cleaning Performance of Metalloprotease PspPro2 in Automatic Dishwashing (ADW) Conditions 
     The cleaning performance of PspPro2 protein in automatic dishwashing (ADW) conditions was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified PspPro2 protein samples were diluted with the dilution solution containing 10 mM NaCl, 0.1 mM CaCl 2 , 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca 2+ :Mg 2+ =3:1), in the presence of a bleach component (Peracid N,N-phthaloylaminoperoxycaproic acid-PAP) (AT detergent composition shown in Table 1). To initiate the reaction, 180 μl of the AT detergent solution at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the “Initial performance”) using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as “Wash-off”). The sum of two absorbance measurements (“Initial performance” plus “Wash-off”) gives the “Total performance”, which measures the protease activity on the model stain. Dose response for cleaning of PA-S-38 microswatches at pH 6 and pH 8 for PspPro2 in AT detergent in the presence of bleach, is shown in  FIGS. 2.5A and 2.5B , respectively. 
     
       
         
           
               
             
               
                 TABLE 2.1 
               
             
            
               
                   
               
               
                 Composition of AT dish detergent with bleach 
               
            
           
           
               
               
            
               
                 Ingredient 
                 Concentration (mg/ml) 
               
               
                   
               
               
                 MGDA (methylglycinediacetic acid) 
                 0.143 
               
               
                 Sodium citrate 
                 1.86  
               
               
                 Citric acid* 
                 varies 
               
               
                 PAP (peracid N, N-phthaloylaminoperoxycaproic acid) 
                 0.057 
               
               
                 Plurafac ® LF 18B (a non-ionic surfactant) 
                 0.029 
               
               
                 Bismuthcitrate 
                 0.006 
               
               
                 Bayhibit ® S (Phosphonobutantricarboxylic acid sodium salt) 
                 0.006 
               
               
                 Acusol ™ 587 (a calcium polyphosphate inhibitor) 
                 0.029 
               
               
                 PEG 6000 
                 0.043 
               
               
                 PEG 1500 
                 0.1  
               
               
                   
               
               
                 *The pH of the AT formula detergent is adjusted to the desired pH value (pH 6 or 8) by the addition of 0.9 M citric acid. 
               
            
           
         
       
     
     Example 2.7 
     Cleaning Performance of Metalloprotease PspPro2 in Laundry Conditions 
     A. Cleaning Performance in Liquid Laundry Detergent 
     The cleaning performance of PspPro2 protein in liquid laundry detergent was tested using EMPA-116 (cotton soiled with blood/milk/ink) microswatches (obtained from CFT Vlaardingen, The Netherlands) at pH 8.2 using a commercial detergent. Prior to the reaction, purified PspPro2 protein samples were diluted with a dilution solution (10 mM NaCl, 0.1 mM CaCl 2 ), 0.005% TWEEN® 80 and 10% propylene glycol) to the desired concentrations; and the commercial detergent (Tide®, Clean Breeze®, Proctor &amp; Gamble, USA, purchased September 2011) was incubated at 95° C. for 1 hour to inactivate the enzymes present in the detergent. Proteolytic assays were subsequently performed to confirm the inactivation of proteases in the commercial detergent. The heat treated detergent was further diluted with 5 mM HEPES (pH 8.2) to a final concentration of 0.788 g/L. Meanwhile, the water hardness of the buffered liquid detergent was adjusted to 103 ppm (Ca 2+ :Mg 2+ =3:1). The specific conductivity of the buffered detergent was adjusted to either 0.62 mS/cm (low conductivity) or 3.5 mS/cm (high conductivity) by adjusting the NaCl concentration in the buffered detergent. To initiate the reaction, 190 μl of either the high or low conductivity buffered detergent was added to a 96-MTP containing the EMPA-116 microswatches, followed by the addition of 10 μl of diluted enzyme (or the dilution solution as blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 20 min at 32° C. and 1150 rpm. After incubation, 150 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 600 nm using a spectrophotometer, which indicates the protease activity on the model stain; and Net A 600  was subsequently calculated by subtracting the A 600  of the blank control from that of the enzyme. Dose response for the cleaning of EMPA-116 microswatches in liquid laundry detergent at high or low conductivity is shown in  FIG. 2.6A . 
     B. Cleaning Performance in Powder Laundry Detergent 
     The cleaning performance of PspPro2 protein in powder laundry detergent was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) using a commercial detergent. Prior to the reaction, purified PspPro2 protein samples were diluted with a dilution solution (10 mM NaCl, 0.1 mM CaCl 2 , 0.005% TWEEN® 80 and 10% propylene glycol) to the desired concentrations. The powder laundry detergent (Tide®, Bleach Free, Proctor &amp; Gamble, China, purchased in December 2011) was dissolved in water with 103 ppm water hardness (Ca 2+ :Mg 2+ =3:1) to a final concentration of 2 g/L (with conductivity of 2.3 mS/cm-low conductivity) or 5 g/L (with conductivity of 5.5 mS/cm-high conductivity). The detergents of different conductivities were subsequently heated in a microwave to near boiling in order to inactivate the enzymes present in the detergent. Proteolytic activity was measured following treatment to ensure that proteases in the commercial detergent had been inactivated. To initiate the reaction, 190 μl of either the high or low conductivity heat-treated detergent was added to a 96-MTP containing the PA-S-38 microswatches, followed by the addition of 10 μl of diluted enzyme (or the dilution solution as blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 15 minutes at 32° C. and 1150 rpm. After incubation, 150 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm using a spectrophotometer, which indicates the protease activity on the model stain; and Net A 405  was subsequently calculated by subtracting the A 405  of the blank control from that of the enzyme. Dose response for the cleaning of PA-S-38 microswatches in powder laundry detergent at high or low conductivity is shown in  FIG. 2.6B . 
     Example 2.8 
     Comparison of PspPro2 to Other Metalloproteases 
     Identification of Homologous Proteases 
     Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The mature protein amino acid sequence for PspPro2 (SEQ ID NO: 8) is used as query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 2.2A and 2.2B provide a list of sequences with the percent identity to PspPro2. The length in Table 2.2 refers to the entire sequence length of the homologous proteases. 
     
       
         
           
               
             
               
                 TABLE 2.2A 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PspPro2 protein identified from 
               
               
                 the NCBI non-redundant protein database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Accession # 
                 PspPro2 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 AAB02774.1 
                  55 
                 
                   Geobacillus stearothermophilus 
                 
                 552 
               
               
                 AAA22623.1 
                  56 
                 
                   Bacillus caldolyticus 
                 
                 544 
               
               
                 P00800 
                  56 
                 
                   Bacillus thermoproteolyticus 
                 
                 548 
               
               
                 YP_003670279.1 
                  57 
                   Geobacillus  sp. C56-T3 
                 546 
               
               
                 BAD60997.1 
                  57 
                 
                   Bacillus megaterium 
                 
                 562 
               
               
                 ZP_02326503.1 
                  58 
                   Paenibacillus larvae  subsp.  larvae  BRL-230010 
                 520 
               
               
                 ZP_08640523.1 
                  58 
                   Brevibacillus laterosporus  LMG 15441 
                 564 
               
               
                 YP_003597483.1 
                  58 
                   Bacillus megaterium  DSM 319 
                 562 
               
               
                 ZP_09069025.1 
                  59 
                   Paenibacillus larvae  subsp.  larvae  B-3650 
                 520 
               
               
                 YP_001373863.1 
                  59 
                   Bacillus cytotoxicus  NVH 391-98 
                 565 
               
               
                 ZP_04149724.1 
                  59 
                   Bacillus pseudomycoides  DSM 12442 
                 566 
               
               
                 CAA43589.1 
                  60 
                 
                   Brevibacillus brevis 
                 
                 527 
               
               
                 ZP_10738945.1 
                  60 
                   Brevibacillus  sp. CF112 
                 528 
               
               
                 ZP_04216147.1 
                  60 
                   Bacillus cereus  Rock3-44 
                 566 
               
               
                 ZP_10575942.1 
                  61 
                   Brevibacillus  sp. BC25 
                 528 
               
               
                 YP_002770810.1 
                  62 
                   Brevibacillus brevis  NBRC 100599 
                 528 
               
               
                 ZP_08511445.1 
                  63 
                   Paenibacillus  sp. HGF7 
                 525 
               
               
                 ZP_09077634.1 
                  64 
                   Paenibacillus elgii  B69 
                 524 
               
               
                 ZP_09071078.1 
                  67 
                   Paenibacillus larvae  subsp.  larvae  B-3650 
                 529 
               
               
                 YP_003872180.1 
                  73 
                   Paenibacillus polymyxa  E681 
                 587 
               
               
                 YP_005073223.1 
                  78 
                   Paenibacillus terrae  HPL-003 
                 591 
               
               
                 ZP_09775364.1 
                  78 
                   Paenibacillus  sp. Aloe-11 
                 593 
               
               
                 YP_003948511.1 
                  80 
                   Paenibacillus polymyxa  SC2 
                 592 
               
               
                 YP_005073224.1 
                  94 
                   Paenibacillus terrae  HPL-003 
                 595 
               
               
                 ZP_10241029.1 
                  96 
                   Paenibacillus peoriae  KCTC 3763 
                 599 
               
               
                 ZP_09775365.1 
                 100 
                   Paenibacillus  sp. Aloe-11 
                 580 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2.2B 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PspPro2 protein identified 
               
               
                 from the Genome Quest database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Patent # 
                 PspPro2 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 JP2002272453-0002 
                 57.01 
                 
                   Bacillus megaterium 
                 
                 562 
               
               
                 US6518054-0001 
                 57.19 
                   Bacillus  sp. 
                 319 
               
               
                 EP2390321-0177 
                 57.19 
                 
                   Bacillus caldolyticus 
                 
                 544 
               
               
                 US20120107907-0176 
                 57.19 
                 
                   Bacillus stearothermophilis 
                 
                 548 
               
               
                 WO9520663-0003 
                 57.51 
                 empty 
                 319 
               
               
                 WO2012110562-0003 
                 57.51 
                 
                   Geobacillus stearothermophilus 
                 
                 319 
               
               
                 WO2012110563-0002 
                 57.51 
                 
                   Bacillus caldolyticus 
                 
                 319 
               
               
                 WO2004011619-0056 
                 57.51 
                 empty 
                 546 
               
               
                 WO2004011619-0003 
                 57.51 
                 empty 
                 546 
               
               
                 JP2002272453-0003 
                 57.64 
                 empty 
                 562 
               
               
                 US6518054-0002 
                 57.88 
                   Bacillus  sp. 
                 316 
               
               
                 EP2178896-0184 
                 58.15 
                 
                   Bacillus anthracis 
                 
                 566 
               
               
                 WO2012110563-0004 
                 58.28 
                 
                   Bacillus megaterium 
                 
                 320 
               
               
                 JP1995184649-0001 
                 58.79 
                   Lactobacillus  sp. 
                 566 
               
               
                 JP1994014788-0003 
                 58.84 
                 empty 
                 317 
               
               
                 US8114656-0178 
                 59.42 
                 
                   Bacillus thuringiensis 
                 
                 566 
               
               
                 WO2012110562-0005 
                 59.49 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 US5962264-0004 
                 59.81 
                 empty 
                 566 
               
               
                 US20120107907-0185 
                 59.81 
                 
                   Bacillus cereus 
                 
                 317 
               
               
                 US20120107907-0179 
                 59.81 
                 
                   Bacillus cereus 
                 
                 566 
               
               
                 WO2012110563-0005 
                 60.13 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 EP2390321-0186 
                 60.33 
                 
                   Bacillus brevis 
                 
                 304 
               
               
                 JP2005229807-0018 
                 78.62 
                 
                   Paenibacillus polymyxa 
                 
                 566 
               
               
                 EP2390321-0187 
                 79.21 
                 
                   Bacillus polymyxa 
                 
                 302 
               
               
                   
               
            
           
         
       
     
     B. Alignment of Homologous Protease Sequences 
     The amino acid sequence of mature PspPro2 (SEQ ID NO: 8) was aligned with thermolysin (P00800,  Bacillus thermoproteolyticus ) and protease from  Paenibacillus  sp. Aloe-11 (ZP_09775365.1) sequences using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters.  FIG. 2.7  shows the alignment of PspPro2 with these protease sequences. 
     C. Phylogenetic Tree 
     A phylogenetic tree for precursor PspPro2 protein sequence (SEQ ID NO: 7) was built using sequences of representative homologs from Table 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. MolBiol.Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in  FIG. 2.8 . 
     Example 3.1 
     Cloning of  Paenibacillus humicus  Metalloprotease PhuPro2 
     A strain (DSM18784) of  Paenibacillus humicus  was selected as a potential source of enzymes which may be useful in various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the  Paenibacillus humicus  strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina&#39;s next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in  Paenibacillus humicus  encodes a metalloprotease and the sequence of this gene, called PhuPro2, is provided in SEQ ID NO: 11. The corresponding protein encoded by the PhuPro2 gene is shown in SEQ ID NO: 12. At the N-terminus, the protein has a signal peptide with a length of 23 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PhuPro2 is a secreted enzyme. The propeptide region was predicted based on its protein sequence alignment with the  Paenibacillus polymyxa  Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PhuPro2 protein in shown in SEQ ID NO: 13. The nucleotide sequence of the PhuPro2 gene isolated from  Paenibacillus humicus  is set forth as SEQ ID NO: 11. The sequence encoding the predicted native signal peptide is shown in italics: 
     
       
         
           
               
            
               
                 
                   ATGAAAAAAATGATTCCTACTCTGCTCGGTACCGTATTGCTGCTTTC 
                 
               
               
                   
               
               
                   TTCCGCTTCCGCTGTCGCTGCT GAATCGCCAAGCCTCGGAGCGGCCG 
               
               
                   
               
               
                 GAACTCCCGGGGTCAGCGTCGTGAACAATCAGCTCGTGACTCAATTC 
               
               
                   
               
               
                 ATCGAGGCTTCCAAGGATGCCAAGATTGTCCCGGGCTCTTCCGAGGA 
               
               
                   
               
               
                 TAAAATCTGGGCTTTCCTTGAAGGCCAGCAAGCAAAGCTGGGTGTAT 
               
               
                   
               
               
                 CCGCAGCGGATGTAAAAACCTCGTTCCTGATCCAGAAGAAGGAAGTC 
               
               
                   
               
               
                 GATCCGACTTCGGGCGTCGAGCATTTCCGCCTGCAGCAATATGTGAA 
               
               
                   
               
               
                 TGGCATCCCGGTATATGGCGGTGACCAAACCATTCACATCGACAAGG 
               
               
                   
               
               
                 CCGGCCAGGTTACGTCGTTCGTAGGAGCTGTTCTGCCGGCTCAAAAT 
               
               
                   
               
               
                 CAAATCACGGCAAAATCCAGCGTACCAGCCATAAGCGCATCCGACGC 
               
               
                   
               
               
                 TCTGGCTATCGCGGCGAAGGAAGCCAGTTCCCGCATCGGCGAGCTGG 
               
               
                   
               
               
                 GAGCACAGGAGAAGACTCCGTCGGCTCAGCTGTACGTATATCCGGAA 
               
               
                   
               
               
                 GGCAACGGGTCGCGTCTCGTCTACCAGACGGAAGTGAATGTGCTTGA 
               
               
                   
               
               
                 GCCGCAGCCTCTGCGCACCCGCTATCTTATCGATGCGGCCGACGGCC 
               
               
                   
               
               
                 ATATCGTGCAGCAGTACGATCTGATCGAGACGGCGACCGGTTCGGGC 
               
               
                   
               
               
                 ACGGGCGTGCTGGGCGACAATAAGACGTTCCAGACGACTCTTTCCGG 
               
               
                   
               
               
                 CAGCACGTACCAGCTGAAAGACACCACTCGCGGCAACGGCATCTACA 
               
               
                   
               
               
                 CCTACACAGCCAGCAATCGGACCACGATTCCGGGCACGCTGCTGACG 
               
               
                   
               
               
                 GACGCCGACAACGTATGGACGGATGGAGCCGCCGTCGATGCCCATAC 
               
               
                   
               
               
                 TTATGCCGGAAAAGTATATGATTTCTACAAAACGAAGTTCGGACGCA 
               
               
                   
               
               
                 ACAGCCTCGACGGCAACGGCCTGCTGATCCGTTCCTCGGTCCACTAC 
               
               
                   
               
               
                 AGCAGCAGGTACAACAATGCCTTCTGGAACGGCACCCAGATTGTATT 
               
               
                   
               
               
                 CGGCGACGGCGACGGCTCGACGTTCATTCCGCTGTCGGGCGATCTCG 
               
               
                   
               
               
                 ACGTGGTCGGCCATGAGCTGTCCCACGGAGTCATCGAGTACACGTCC 
               
               
                   
               
               
                 AACCTTCAATACCTCAATGAATCCGGCGCGCTGAACGAGTCCTATGC 
               
               
                   
               
               
                 CGACGTCCTCGGCAACTCGATCCAGGCGAAAAACTGGCTTATCGGCG 
               
               
                   
               
               
                 ACGATGTCTATACGCCTGGCATCTCCGGAGATGCTCTCCGTTCCATG 
               
               
                   
               
               
                 TCCAACCCGACGCTTTACGGGCAGCCGGACAACTATGCCAACCGCTA 
               
               
                   
               
               
                 TACGGGATCTTCCGACAACGGCGGCGTTCATACGAACAGCGGCATCA 
               
               
                   
               
               
                 CGAACAAAGCGTTCTACCTGCTCGCCCAAGGCGGCACCCAGAACGGC 
               
               
                   
               
               
                 GTTACCGTCGCCGGCATCGGGCGCGACGCAGCCGTGAACATTTTCTA 
               
               
                   
               
               
                 CAACACAGTGGCCTATTACCTTACTTCCACTTCCAACTTCGCCGCGG 
               
               
                   
               
               
                 CGAAGAACGCCTCGATCCAGGCAGCCAAAGACCTGTACGGAACGGGC 
               
               
                   
               
               
                 TCCTCTTATGTCACCTCGGTGACCAATGCATTCAGAGCCGTAGGCCT 
               
               
                   
               
               
                 G 
               
            
           
         
       
     
     The amino acid sequence of the PhuPro2 precursor protein is set forth as SEQ ID NO: 12. The predicted signal sequence is shown in italics, and the predicted propeptide is shown in underlined text: 
                      MKKMIPTLLGTVLLLSSASAVAA   ESPSLGAAGTPGVSVVNNQLVTQF                   IEASKDAKIVPGSSEDKIWAFLEGQQAKLGVSAADVKTSFLIQKKEV                   DPTSGVEHFRLQQYVNGIPVYGGDQTIHIDKAGQVTSFVGAVLPAQN                   QITAKSSVPAISASDALAIAAKEASSRIGELGAQEKTPSAQLYVYPE                   GNGSRLVYQTEVNVLEPQPLRTRYLIDAADGHIVQQYDLIET ATGSG               TGVLGDNKTFQTTLSGSTYQLKDTTRGNGIYTYTASNRTTIPGTLLT               DADNVWTDGAAVDAHTYAGKVYDFYKTKFGRNSLDGNGLLIRSSVHY               SSRYNNAFWNGTQIVFGDGDGSTFIPLSGDLDVVGHELSHGVIEYTS               NLQYLNESGALNESYADVLGNSIQAKNWLIGDDVYTPGISGDALRSM               SNPTLYGQPDNYANRYTGSSDNGGVHTNSGITNKAFYLLAQGGTQNG               VTVAGIGRDAAVNIFYNTVAYYLTSTSNFAAAKNASIQAAKDLYGTG               SSYVTSVTNAFRAVGL            
The amino acid sequence of the predicted mature form of PhuPro2 is set forth as SEQ ID NO: 13:
 
     
       
         
           
               
            
               
                 ATGSGTGVLGDNKTFQTTLSGSTYQLKDTTRGNGIYTYTASNRTTIP 
               
               
                   
               
               
                 GTLLTDADNVWTDGAAVDAHTYAGKVYDFYKTKFGRNSLDGNGLLIR 
               
               
                   
               
               
                 SSVHYSSRYNNAFWNGTQIVFGDGDGSTFIPLSGDLDVVGHELSHGV 
               
               
                   
               
               
                 IEYTSNLQYLNESGALNESYADVLGNSIQAKNWLIGDDVYTPGISGD 
               
               
                   
               
               
                 ALRSMSNPTLYGQPDNYANRYTGSSDNGGVHTNSGITNKAFYLLAQG 
               
               
                   
               
               
                 GTQNGVTVAGIGRDAAVNIFYNTVAYYLTSTSNFAAAKNASIQAAKD 
               
               
                   
               
               
                 LYGTGSSYVTSVTNAFRAVGL 
               
            
           
         
       
     
     Example 3.2 
     Expression of  Paenibacillus humicus  s Metalloprotease PhuPro2 
     The DNA sequence of the propeptide-mature form of PhuPro2 was synthesized and inserted into the  Bacillus subtilis  expression vector p2JM103BBI (Vogtentanz,  Protein Expr Purif,  55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX150(AprE-PhuPro2) ( FIG. 1 ). Ligation of this gene encoding the PhuPro2 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3′ end of the  B. subtilis  AprE signal sequence and the 5′ end of the predicted PhuPro2 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in  FIG. 1  was labeled pGX150(AprE-PhuPro2). As shown in  FIG. 3.1 , pGX150(AprE-PhuPro2) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in  B. subtilis , and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PhuPro2 (SEQ ID NO: 14). The translation product of the synthetic AprE-PhuPro2 gene is shown in SEQ ID NO: 15. 
     The pGX150 (AprE-PhuPro2) plasmid was then transformed into  B. subtilis  cells (degU Hy  32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat #232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl 2 ). 
     The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl 2  and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to an 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above; and the active flow-through fractions were collected and concentrated. The sample was loaded onto a 320 ml Superdex 75 gel filtration column pre-equilibrated with the loading buffer described above containing 0.15 M NaCl. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses. 
     The nucleotide sequence of the synthesized PhuPro2 gene in plasmid pGX150(AprE-PhuPro2) is depicted in SEQ ID NO: 14. The sequence encoding the three residue addition (AGK) is shown in bold: 
     
       
         
           
               
            
               
                 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTT 
               
               
                   
               
               
                 AATCTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCT GCTGGAA   
               
               
                   
               
               
                   AA GAATCACCGAGCCTTGGCGCTGCAGGAACACCGGGCGTTAGCGTT 
               
               
                   
               
               
                 GTGAATAACCAACTGGTCACGCAGTTCATCGAAGCATCAAAAGACGC 
               
               
                   
               
               
                 GAAAATTGTCCCTGGATCAAGCGAAGATAAGATTTGGGCATTTCTGG 
               
               
                   
               
               
                 AAGGCCAGCAAGCAAAGCTTGGCGTCTCAGCTGCCGACGTGAAGACG 
               
               
                   
               
               
                 AGCTTCCTGATCCAGAAGAAGGAGGTTGACCCGACATCAGGCGTTGA 
               
               
                   
               
               
                 GCACTTTAGACTGCAACAGTACGTCAACGGCATCCCGGTTTATGGAG 
               
               
                   
               
               
                 GCGATCAAACAATCCATATTGATAAGGCAGGCCAGGTCACATCATTC 
               
               
                   
               
               
                 GTCGGAGCTGTCCTGCCGGCTCAGAACCAAATTACAGCAAAATCATC 
               
               
                   
               
               
                 AGTTCCGGCAATTTCAGCCTCAGACGCTCTGGCAATCGCTGCCAAGG 
               
               
                   
               
               
                 AGGCAAGCTCAAGAATTGGCGAACTGGGCGCACAAGAAAAGACACCG 
               
               
                   
               
               
                 AGCGCCCAACTTTATGTCTATCCGGAGGGCAACGGAAGCAGACTGGT 
               
               
                   
               
               
                 GTACCAGACAGAGGTCAATGTTCTGGAGCCGCAACCGCTGAGAACGA 
               
               
                   
               
               
                 GATACCTTATCGATGCTGCGGATGGCCACATTGTTCAGCAATACGAC 
               
               
                   
               
               
                 CTGATTGAGACAGCAACAGGAAGCGGAACGGGCGTGCTGGGCGACAA 
               
               
                   
               
               
                 CAAGACGTTTCAGACAACACTTAGCGGCAGCACGTACCAACTTAAGG 
               
               
                   
               
               
                 ACACGACGAGAGGCAATGGCATTTACACGTACACGGCCTCAAACAGA 
               
               
                   
               
               
                 ACGACAATCCCAGGCACACTGCTGACGGATGCAGACAATGTTTGGAC 
               
               
                   
               
               
                 GGACGGCGCAGCAGTTGACGCACACACGTACGCCGGCAAGGTGTACG 
               
               
                   
               
               
                 ACTTTTACAAGACGAAGTTCGGCAGAAACAGCCTTGATGGAAATGGA 
               
               
                   
               
               
                 CTGCTGATCAGAAGCAGCGTCCACTACAGCAGCAGATACAATAACGC 
               
               
                   
               
               
                 CTTCTGGAACGGCACACAAATCGTCTTTGGCGATGGAGACGGATCAA 
               
               
                   
               
               
                 CATTCATCCCGCTGTCAGGCGACCTGGACGTTGTGGGCCACGAGCTG 
               
               
                   
               
               
                 AGCCACGGCGTCATCGAGTACACGAGCAACCTGCAGTACCTGAATGA 
               
               
                   
               
               
                 AAGCGGCGCACTGAACGAGTCATATGCTGATGTGCTTGGCAATAGCA 
               
               
                   
               
               
                 TCCAGGCCAAGAACTGGCTTATCGGAGACGACGTCTACACACCTGGC 
               
               
                   
               
               
                 ATCAGCGGCGATGCTCTGAGAAGCATGAGCAATCCTACACTTTACGG 
               
               
                   
               
               
                 CCAACCGGACAACTACGCGAATAGATATACGGGCAGCAGCGACAATG 
               
               
                   
               
               
                 GCGGCGTTCATACAAACTCAGGCATCACGAACAAGGCGTTCTACCTG 
               
               
                   
               
               
                 CTGGCACAGGGAGGCACGCAAAACGGCGTTACAGTTGCGGGCATTGG 
               
               
                   
               
               
                 CAGAGATGCGGCCGTCAACATCTTCTACAACACAGTCGCCTACTACC 
               
               
                   
               
               
                 TGACGAGCACGTCAAACTTCGCAGCGGCAAAGAACGCATCAATTCAA 
               
               
                   
               
               
                 GCAGCAAAGGATCTGTACGGAACAGGCAGCTCATATGTCACGTCAGT 
               
               
                   
               
               
                 TACGAATGCGTTTAGAGCCGTCGGCCTTTAA 
               
            
           
         
       
     
     The amino acid sequence of the PhuPro2 precursor protein expressed from plasmid pGX150(AprE-PhuPro2) is depicted in SEQ ID NO: 15. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted propeptide is shown in underlined text. 
     
       
         
           
               
            
               
                 (SEQ ID NO: 15) 
               
               
                 
                   MRSKKLWISLLFALTLIFTMAFSNMSAQA 
                   AGK 
                   ESPSLGAAGTPGVSV 
                 
               
               
                   
               
               
                 
                   VNNQLVTQFIEASKDAKIVPGSSEDKIWAFLEGQQAKLGVSAADVKT 
                 
               
               
                   
               
               
                 
                   SFLIQKKEVDPTSGVEHFRLQQYVNGIPVYGGDQTIHIDKAGQVTSF 
                 
               
               
                   
               
               
                 
                   VGAVLPAQNQITAKSSVPAISASDALAIAAKEASSRIGELGAQEKTP 
                 
               
               
                   
               
               
                 
                   SAQLYVYPEGNGSRLVYQTEVNVLEPQPLRTRYLIDAADGHIVQQYD 
                 
               
               
                   
               
               
                   LIET ATGSGTGVLGDNKTFQTTLSGSTYQLKDTTRGNGIYTYTASNR 
               
               
                   
               
               
                 TTIPGTLLTDADNVWTDGAAVDAHTYAGKVYDFYKTKFGRNSLDGNG 
               
               
                   
               
               
                 LLIRSSVHYSSRYNNAFWNGTQIVFGDGDGSTFIPLSGDLDVVGHEL 
               
               
                   
               
               
                 SHGVIEYTSNLQYLNESGALNESYADVLGNSIQAKNWLIGDDVYTPG 
               
               
                   
               
               
                 ISGDALRSMSNPTLYGQPDNYANRYTGSSDNGGVHTNSGITNKAFYL 
               
               
                   
               
               
                 LAQGGTQNGVTVAGIGRDAAVNIFYNTVAYYLTSTSNFAAAKNASIQ 
               
               
                   
               
               
                 AAKDLYGTGSSYVTSVTNAFRAVGL. 
               
            
           
         
       
     
     Example 3.3 
     Proteolytic Activity of Metalloprotease PhuPro2 
     The proteolytic activity of purified metalloprotease PhuPro2 was measured in 50 mM Tris (pH 7), using azo-casein (Cat #74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H 2 O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A 440 ) using a SpectraMax 190. Net A 440  was calculated by subtracting the A 440  of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of triplicate assays. 
     The proteolytic activity is shown as Net A 440 . The proteolytic assay with azo-casein as the substrate (shown in  FIG. 3.2 ) indicates that PhuPro2 is an active protease. 
     Example 3.4 
     pH Profile of Metalloprotease PhuPro2 
     With azo-casein as the substrate, the pH profile of metalloprotease PhuPro2 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl Milli-Q H 2 O diluted enzyme (125 ppm) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 3.3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in  FIG. 3.3 , the optimal pH of PhuPro2 is 6, with greater than 70% of maximal activity retained between 5.5 and 8.5. 
     Example 3.5 
     Temperature Profile of Metalloprotease PhuPro2 
     The temperature profile of metalloprotease PhuPro2 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assays. The enzyme sample and azo-casein substrate were prepared as in Example 3.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H 2 O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20-90° C.) for 5 min. After the incubation, 5 μl of diluted enzyme (50 ppm) or H 2 O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 3.3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in  FIG. 3.4  suggests that PhuPro2 showed an optimal temperature at 50° C., and retained greater than 70% of its maximum activity between 45 and 65° C. 
     Example 3.6 
     Cleaning Performance of Metalloprotease PhuPro2 
     The cleaning performance of PhuPro2 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified protease samples were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl 2 , 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca 2+ :Mg 2+ =3:1) (detergent composition shown in Table 3.1). To initiate the reaction, 180 μl of the AT detergent buffered at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the “Initial performance”) using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as the “Wash-off”). The sum of two absorbance measurements (“Initial performance” plus “Wash-off”) gives the “Total performance”, which measures the protease activity on the model stain; and Net A 405  was subsequently calculated by subtracting the A 405  of the “Total performance” of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 in AT dish detergent for PhuPro2 is shown in  FIGS. 3.5A and 3.5B . 
     
       
         
           
               
             
               
                 TABLE 3.1 
               
             
            
               
                   
               
               
                 Composition of AT dish detergent 
               
            
           
           
               
               
            
               
                 Ingredient 
                 Concentration (mg/ml) 
               
               
                   
               
               
                 MGDA (methylglycinediacetic acid) 
                 0.143 
               
               
                 Sodium citrate 
                 1.86  
               
               
                 Citric acid* 
                 varies 
               
               
                 Plurafac ® LF 18B (a non-ionic surfactant) 
                 0.029 
               
               
                 Bismuthcitrate 
                 0.006 
               
               
                 Bayhibit ® S (Phosphonobutantricarboxylic acid sodium salt) 
                 0.006 
               
               
                 Acusol ™ 587 (a calcium polyphosphate inhibitor) 
                 0.029 
               
               
                 PEG 6000 
                 0.043 
               
               
                 PEG 1500 
                 0.1  
               
               
                   
               
               
                 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9 M citric acid. 
               
            
           
         
       
     
     Example 3.7 
     Comparison of PhuPro2 to Other Proteases 
     A. Identification of Homologous Proteases 
     Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The predicted mature protein amino acid sequence for PhuPro2 (SEQ ID NO: 13) is used as the query sequences. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 3.2A and 3.2B provide a list of sequences with the percent identity to PhuPro2. The length in Table 3.2 refers to the entire sequence length of the homologous proteases. 
     
       
         
           
               
             
               
                 TABLE 3.2A 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PhuPro2 protein identified from 
               
               
                 the NCBI non-redundant protein database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Accession # 
                 PhuPro2 
                 Organism 
                 Length 
               
               
                   
               
               
                 P00800 
                 59 
                 
                   Bacillus thermoproteolyticus 
                 
                 548 
               
               
                 YP_003872180.1 
                 59 
                   Paenibacillus polymyxa  E681 
                 587 
               
               
                 ZP_10575942.1 
                 59 
                   Brevibacillus  sp. BC25 
                 528 
               
               
                 ZP_02326602.1 
                 60 
                   Paenibacillus larvae  subsp.  larvae   
                 520 
               
               
                   
                   
                 BRL-230010 
                   
               
               
                 ADM87306.1 
                 61 
                 
                   Bacillus megaterium 
                 
                 562 
               
               
                 ZP_09069025.1 
                 61 
                   Paenibacillus larvae  subsp. 
                 520 
               
               
                   
                   
                   larvae  B-3650 
                   
               
               
                 ZP_09069194.1 
                 62 
                   Paenibacillus larvae  subsp. 
                 502 
               
               
                   
                   
                   Larvae  B-3650 
                   
               
               
                 ZP_10738945.1 
                 63 
                   Brevibacillus  sp. CF112 
                 528 
               
               
                 ZP_08511445.1 
                 64 
                   Paenibacillus  sp. HGF7 
                 525 
               
               
                 ZP_09077634.1 
                 65 
                   Paenibacillus elgii  B69 
                 524 
               
               
                 ZP_09775365.1 
                 65 
                   Paenibacillus  sp. Aloe-11 
                 580 
               
               
                 ZP_09775364.1 
                 70 
                   Paenibacillus  sp. Aloe-11 
                 593 
               
               
                 P29148 
                 71 
                 
                   Paenibacillus polymyxa 
                 
                 590 
               
               
                 ZP_10241030.1 
                 71 
                   Paenibacillus peoriae  KCTC 3763 
                 593 
               
               
                 ZP_09071078.1 
                 71 
                   Paenibacillus larvae  subsp. 
                 529 
               
               
                   
                   
                   larvae  B-3650 
                   
               
               
                 YP_003872179.1 
                 72 
                   Paenibacillus polymyxa  E681 
                 592 
               
               
                 YP_005073223.1 
                 72 
                   Paenibacillus terrae  HPL-003 
                 591 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3.2B 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PhuPro2 protein identified 
               
               
                 from the Genome Quest Patent database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Patent ID # 
                 PhuPro2 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 US20090208474-0030 
                 59.22 
                 
                   Bacillus thermoproteolyticus 
                 
                 316 
               
               
                 JP2002272453-0002 
                 59.42 
                 
                   Bacillus megaterium 
                 
                 562 
               
               
                 JP2006124323-0003 
                 59.55 
                 
                   Bacillus thermoproteolyticus 
                 
                 316 
               
               
                 US8114656-0183 
                 59.87 
                 
                   Bacillus stearothermophilis 
                 
                 316 
               
               
                 JP1989027475-0001 
                 59.87 
                 
                   Bacillus subtilis 
                 
                 316 
               
               
                 US20120009651-0002 
                 59.87 
                 
                   Geobacillus caldoproteolyticus 
                 
                 548 
               
               
                 JP2002272453-0003 
                 60.45 
                 empty 
                 562 
               
               
                 WO2012110563-0004 
                 60.77 
                 
                   Bacillus megaterium 
                 
                 320 
               
               
                 EP2390321-0186 
                 62.25 
                 
                   Bacillus brevis 
                 
                 304 
               
               
                 JP2005229807-0018 
                 71.85 
                 
                   Paenibacillus polymyxa 
                 
                 566 
               
               
                 US8114656-0187 
                 72.09 
                 
                   Bacillus polymyxa 
                 
                 302 
               
               
                   
               
            
           
         
       
     
     B. Alignment of Homologous Protease Sequences 
     The amino acid sequence of predicted mature PhuPro2 (SEQ ID NO: 13) protein was aligned with thermolysin (P00800,  Bacillus thermoproteolyticus ), and protease from  Paenibacillus terrae  HPL-003 (YP 005073223.1) sequences using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters.  FIG. 3.6  shows the alignment of PhuPro2 with these protease sequences. 
     C. Phylogenetic Tree 
     A phylogenetic tree for full length sequence of PhuPro2 (SEQ ID NO: 12) was built using sequences of representative homologs from Table 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. MolBiol.Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in  FIG. 3.7 . 
     Example 4.1 
     Cloning of  Paenibacillus ehimensis  Metalloprotease PehPro1 
     A strain (DSM11029) of  Paenibacillus ehimensis  was selected as a potential source of enzymes which may be useful in various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the  Paenibacillus ehimensis  strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina&#39;s next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in  Paenibacillus ehimensis  encodes a metalloprotease and the sequence of this gene, called PehPro1, is provided in SEQ ID NO: 16. The corresponding protein encoded by the PehPro1 gene is shown in SEQ ID NO: 17. At the N-terminus, the protein has a signal peptide with a length of 23 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PehPro1 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the  Paenibacillus polymyxa  Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PehPro1 protein is shown in SEQ ID NO: 18. 
     The nucleotide sequence of the PehPro1 gene isolated from  Paenibacillus ehimensis  is set forth as SEQ ID NO: 16. The sequence encoding the predicted native signal peptide is shown in italics: 
     
       
         
           
               
            
               
                 
                   ATGTTAAAAGTATGGGCATCGATTATTACAGGAGCATTTTTGCTCGG 
                 
               
               
                   
               
               
                   GAGCGTGCAAGGGGTGCAAGCT GCTCCACAAGATCAAGCTGCTCCCT 
               
               
                   
               
               
                 TCGGAGGATTCACCCCTCAATTGATTACCGGGGAAAGCTGGAGTGCG 
               
               
                   
               
               
                 CCGCAAGGAGTATCGGGAGAGGAAAAAATCTGGAAGTATCTCGAATC 
               
               
                   
               
               
                 CAAGCAGGAAAGCTTCCAAATCGGCCAAACCGTTGATCTGAAAAAGC 
               
               
                   
               
               
                 AATTGAAAATTATCGGCCAAACGACCGACGAGAAAACGGGAACCACG 
               
               
                   
               
               
                 CATTACCGTCTACAGCAGTATGTGGGAGGCGTCCCCGTATACGGCGG 
               
               
                   
               
               
                 CGTACAAACGATCCATGTCAACAAAGAAGGACAAGTTACCTCGCTGA 
               
               
                   
               
               
                 TCGGCAGCCTGCTTCCCGACCAGCAGCAGCAAGTTTCGAAAAGCTTG 
               
               
                   
               
               
                 AATTCGCAAATCAGCGAAGCGCAAGCCATCGCCGTGGCCCAGAAAGA 
               
               
                   
               
               
                 TACCGAGGCCGCCGTCGGCAAGCTGGGTGAACCGCAAAAGACACCGG 
               
               
                   
               
               
                 AAGCGGATCTGTACGTTTATTTACACAACGGACAACCGGTCCTCGCT 
               
               
                   
               
               
                 TATGTGACCGAGGTTAACGTTCTCGAACCGGAGGCAATCCGGACGCG 
               
               
                   
               
               
                 CTACTTCATCAGCGCCGAAGACGGCAGCATTTTATTCAAGTACGACA 
               
               
                   
               
               
                 TCCTCGCTCACGCTACAGGTACCGGAAAAGGCGTGCTCGGAGATACG 
               
               
                   
               
               
                 AAATCGTTCACGACCACGCAATCCGGCTCCACTTATCAATTGAAGGA 
               
               
                   
               
               
                 TACGACGCGCGGGCAAGGTATCGTCACTTACAGCGCTGGCAACCGGT 
               
               
                   
               
               
                 CCTCTCTGCCGGGAACGCTGCTCACCAGCTCCAGCAATATTTGGAAC 
               
               
                   
               
               
                 GACGGCGCGGCGGTCGATGCGCATGCCTATACCGCCAAAGTGTACGA 
               
               
                   
               
               
                 TTACTATAAAAACAAATTTGGCCGCAACAGCATTGACGGCAACGGCT 
               
               
                   
               
               
                 TCCAGCTTAAATCGACCGTGCACTATTCCTCCAGATACAACAACGCC 
               
               
                   
               
               
                 TTCTGGAACGGTGTGCAAATGGTGTACGGCGACGGCGACGGCGTAAC 
               
               
                   
               
               
                 CTTCATTCCGTTCTCCGCCGATCCGGACGTCATCGGCCACGAATTGA 
               
               
                   
               
               
                 CCCACGGCGTTACGGAACATACGGCCGGCCTGGAATACTACGGCGAA 
               
               
                   
               
               
                 TCCGGAGCGCTGAACGAATCGATCTCCGATATTATCGGCAACGCGAT 
               
               
                   
               
               
                 CGACGGCAAAAACTGGCTGATCGGCGACTTGATTTATACGCCGAATA 
               
               
                   
               
               
                 CTCCCGGGGACGCCCTCCGCTCTATGGAGAACCCCAAGCTGTATAAC 
               
               
                   
               
               
                 CAACCCGACCGCTATCAAGACCGCTATACGGGACCTTCCGATAACGG 
               
               
                   
               
               
                 CGGCGTGCATATTAACAGCGGTATCAACAACAAAGCCTTCTACCTGA 
               
               
                   
               
               
                 TCGCCCAAGGCGGCACGCACTATGGCGTCACCGTGAACGGGATCGGA 
               
               
                   
               
               
                 CGCGATGCGGCTGTGCAAATTTTCTATGACGCCCTCATCAATTACCT 
               
               
                   
               
               
                 GACTCCAACTTCGAACTTCTCGGCGATGCGCGCAGCAGCCATTCAAG 
               
               
                   
               
               
                 CGGCAACCGACCTGTACGGAGCGAATTCTTCTCAAGTAAACGCTGTC 
               
               
                   
               
               
                 AAAAAAGCGTATACTGCCGTCGGCGTGAAC 
               
            
           
         
       
     
     The amino acid sequence of the PehPro1 precursor protein is set forth as SEQ ID NO: 17. The predicted signal sequence is shown in italics, and the predicted propeptide is shown in underlined text: 
     
       
         
           
               
            
               
                 MLKVWASIITGAFLLGSVQGVQA APQDQAAPFGGFTPQLITGESWSA   
               
               
                   
               
               
                 
                   PQGVSGEEKIWKYLESKQESFQIGQTVDLKKQLKIIGQTTDEKTGTT 
                 
               
               
                   
               
               
                 
                   HYRLQQYVGGVPVYGGVQTIHVNKEGQVTSLIGSLLPDQQQQVSKSL 
                 
               
               
                   
               
               
                 
                   NSQISEAQAIAVAQKDTEAAVGKLGEPQKTPEADLYVYLHNGQPVLA 
                 
               
               
                   
               
               
                   YVTEVNVLEPEAIRTRYFISAEDGSILFKYDILAH ATGTGKGVLGDT 
               
               
                   
               
               
                 KSFTTTQSGSTYQLKDTTRGQGIVTYSAGNRSSLPGTLLTSSSNIWN 
               
               
                   
               
               
                 DGAAVDAHAYTAKVYDYYKNKFGRNSIDGNGFQLKSTVHYSSRYNNA 
               
               
                   
               
               
                 FWNGVQMVYGDGDGVTFIPFSADPDVIGHELTHGVTEHTAGLEYYGE 
               
               
                   
               
               
                 SGALNESISDIIGNAIDGKNWLIGDLIYTPNTPGDALRSMENPKLYN 
               
               
                   
               
               
                 QPDRYQDRYTGPSDNGGVHINSGINNKAFYLIAQGGTHYGVTVNGIG 
               
               
                   
               
               
                 RDAAVQIFYDALINYLTPTSNFSAMRAAAIQAATDLYGANSSQVNAV 
               
               
                   
               
               
                 KKAYTAVGVN 
               
            
           
         
       
     
     The amino acid sequence of the predicted mature form of PehPro1 is set forth as SEQ ID NO: 18: 
     
       
         
           
               
            
               
                 ATGTGKGVLGDTKSFTTTQSGSTYQLKDTTRGQGIVTYSAGNRSSLP 
               
               
                   
               
               
                 GTLLTSSSNIWNDGAAVDAHAYTAKVYDYYKNKFGRNSIDGNGFQLK 
               
               
                   
               
               
                 STVHYSSRYNNAFWNGVQMVYGDGDGVTFIPFSADPDVIGHELTHGV 
               
               
                   
               
               
                 TEHTAGLEYYGESGALNESISDIIGNAIDGKNWLIGDLIYTPNTPGD 
               
               
                   
               
               
                 ALRSMENPKLYNQPDRYQDRYTGPSDNGGVHINSGINNKAFYLIAQG 
               
               
                   
               
               
                 GTHYGVTVNGIGRDAAVQIFYDALINYLTPTSNFSAMRAAAIQAATD 
               
               
                   
               
               
                 LYGANSSQVNAVKKAYTAVGVN 
               
            
           
         
       
     
     Example 4.2 
     Expression of  Paenibacillus ehimensis  Metalloprotease PehPro1 
     The DNA sequence of the propeptide-mature form of PehPro1 was synthesized and inserted into the  Bacillus subtilis  expression vector p2JM103BBI (Vogtentanz,  Protein Expr Purif,  55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX148(AprE-PehPro1) ( FIG. 4.1 ). Ligation of this gene encoding the PehPro1 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3′ end of the  B. subtilis  AprE signal sequence and the 5′ end of the predicted PehPro1 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in  FIG. 1  was labeled pGX148(AprE-PehPro1). As shown in  FIG. 1 , pGX148(AprE-PehPro1) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in  B. subtilis , and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PehPro1 (SEQ ID NO: 19). The translation product of the synthetic AprE-PehPro1 gene is shown in SEQ ID NO: 20. 
     The pGX148(AprE-PehPro1) plasmid was then transformed into  B. subtilis  cells (degU Hy  32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat #232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium. supplemented with additional 5 mM CaCl 2 )). 
     The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl 2 ) and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to an 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above, PehPro1 was eluted from the column with a linear salt gradient from 0 to 0.3 M NaCl in the loading buffer. The corresponding active fractions were collected, concentrated and buffer-exchanged again into the loading buffer described above. The sample was loaded onto a 40 ml DEAE Fast Flow column pre-equilibrated with the same loading buffer. PehPro1 was eluted from the column with a linear salt gradient from 0 to 0.15 M NaCl in the loading buffer. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses. 
     The nucleotide sequence of the synthesized PehPro1 gene in plasmid pGX148(AprE-PehPro1) is depicted in SEQ ID NO: 19. The sequence encoding the three residue addition (AGK) is shown in bold: 
     
       
         
           
               
            
               
                 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTT 
               
               
                   
               
               
                 AATCTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCT GCTGGAA   
               
               
                   
               
               
                   AA GCACCTCAAGATCAGGCAGCACCTTTTGGAGGCTTTACACCGCAA 
               
               
                   
               
               
                 CTTATCACAGGCGAATCATGGTCAGCACCGCAGGGCGTTTCAGGCGA 
               
               
                   
               
               
                 GGAAAAGATCTGGAAGTACCTTGAGAGCAAGCAGGAGTCATTTCAAA 
               
               
                   
               
               
                 TCGGCCAGACAGTCGACCTGAAAAAGCAACTGAAGATCATCGGCCAA 
               
               
                   
               
               
                 ACAACGGACGAAAAGACGGGCACGACGCATTATAGACTGCAACAATA 
               
               
                   
               
               
                 TGTTGGCGGCGTGCCGGTTTATGGAGGCGTGCAAACAATCCACGTGA 
               
               
                   
               
               
                 ACAAGGAAGGACAGGTCACGTCACTGATCGGCAGCCTGCTGCCGGAT 
               
               
                   
               
               
                 CAGCAGCAACAAGTCTCAAAGAGCCTGAACTCACAAATTAGCGAGGC 
               
               
                   
               
               
                 ACAAGCGATTGCAGTTGCACAAAAGGACACGGAAGCAGCTGTCGGCA 
               
               
                   
               
               
                 AGCTGGGCGAACCGCAAAAAACACCTGAGGCTGACCTTTACGTCTAC 
               
               
                   
               
               
                 CTGCATAACGGCCAGCCGGTCCTTGCGTACGTTACGGAAGTTAACGT 
               
               
                   
               
               
                 GCTGGAGCCGGAGGCCATCAGAACGAGATACTTCATTAGCGCGGAGG 
               
               
                   
               
               
                 ATGGAAGCATTCTGTTTAAGTACGATATTCTTGCTCACGCGACAGGC 
               
               
                   
               
               
                 ACAGGCAAGGGCGTCCTTGGCGACACAAAAAGCTTCACGACAACGCA 
               
               
                   
               
               
                 GAGCGGATCAACGTACCAGCTGAAAGATACAACAAGAGGACAAGGCA 
               
               
                   
               
               
                 TCGTTACGTATTCAGCGGGCAATAGATCAAGCCTGCCGGGCACACTG 
               
               
                   
               
               
                 CTGACATCAAGCTCAAACATTTGGAATGACGGCGCAGCAGTTGATGC 
               
               
                   
               
               
                 CCATGCGTACACAGCCAAGGTGTACGACTACTATAAGAACAAGTTTG 
               
               
                   
               
               
                 GCAGAAATAGCATCGACGGAAATGGATTTCAACTTAAATCAACGGTG 
               
               
                   
               
               
                 CACTACTCATCAAGATATAACAATGCGTTTTGGAACGGAGTGCAGAT 
               
               
                   
               
               
                 GGTCTACGGAGACGGCGACGGCGTGACATTTATTCCGTTTAGCGCCG 
               
               
                   
               
               
                 ACCCGGACGTGATTGGACATGAACTGACACATGGAGTGACAGAGCAT 
               
               
                   
               
               
                 ACGGCGGGACTGGAATATTACGGCGAAAGCGGCGCACTGAACGAAAG 
               
               
                   
               
               
                 CATCTCAGACATTATTGGAAACGCAATCGATGGCAAAAACTGGCTGA 
               
               
                   
               
               
                 TTGGCGATCTGATTTATACGCCGAATACACCGGGCGATGCACTGAGA 
               
               
                   
               
               
                 TCAATGGAGAATCCGAAGCTGTACAACCAACCGGACAGATACCAAGA 
               
               
                   
               
               
                 TAGATACACAGGACCGTCAGACAACGGCGGAGTCCATATCAACAGCG 
               
               
                   
               
               
                 GAATCAATAACAAAGCCTTTTACCTGATCGCCCAAGGCGGAACGCAC 
               
               
                   
               
               
                 TATGGCGTTACAGTCAATGGCATCGGAAGAGATGCCGCAGTTCAGAT 
               
               
                   
               
               
                 TTTCTATGACGCGCTGATCAACTATCTGACGCCTACAAGCAATTTCT 
               
               
                   
               
               
                 CAGCAATGAGAGCCGCAGCAATCCAAGCAGCCACGGATCTGTATGGA 
               
               
                   
               
               
                 GCCAATTCATCACAAGTTAATGCTGTTAAGAAGGCTTATACGGCAGT 
               
               
                   
               
               
                 GGGAGTTAACTAA 
               
            
           
         
       
     
     The amino acid sequence of the PehPro1 precursor protein expressed from plasmid pGX148(AprE-PehPro1) is depicted in SEQ ID NO: 20. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text. 
     
       
         
           
               
            
               
                 
                   MRSKKLWISLLFALTLIFTMAFSNMSAQA 
                   AGK 
                   APQDQAAPFGGFTPQ 
                 
               
               
                   
               
               
                 
                   LITGESWSAPQGVSGEEKIWKYLESKQESFQIGQTVDLKKQLKIIGQ 
                 
               
               
                   
               
               
                 
                   TTDEKTGTTHYRLQQYVGGVPVYGGVQTIHVNKEGQVTSLIGSLLPD 
                 
               
               
                   
               
               
                 
                   QQQQVSKSLNSQISEAQAIAAQKDTEAAVGKLGEPQKTPEADLYVYL 
                 
               
               
                   
               
               
                   HNGQPVLAYVTEVNVLEPEAIRTRYFISAEDGSILFKYDILAH ATGT 
               
               
                   
               
               
                 GKGVLGDTKSFTTTQSGSTYQLKDTTRGQGIVTYSAGNRSSLPGTLL 
               
               
                   
               
               
                 TSSSNIWNDGAAVDAHAYTAKVYDYYKNKFGRNSIDGNGFQLKSTVH 
               
               
                   
               
               
                 YSSRYNNAFWNGVQMVYGDGDGVTFIPFSADPDVIGHELTHGVTEHT 
               
               
                   
               
               
                 AGLEYYGESGALNESISDIIGNAIDGKNWLIGDLIYTPNTPGDALRS 
               
               
                   
               
               
                 MENPKLYNQPDRYQDRYTGPSDNGGVHINSGINNKAFYLIAQGGTHY 
               
               
                   
               
               
                 GVTVNGIGRDAAVQIFYDALINYLTPTSNFSAMRAAAIQAATDLYGA 
               
               
                   
               
               
                 NSSQVNAVKKAYTAVGVN. 
               
            
           
         
       
     
     Example 4.3 
     Proteolytic Activity of Metalloprotease PehPro1 
     The proteolytic activity of purified metalloprotease PehPro1 was measured in 50 mM Tris (pH 7), using azo-casein (Cat #74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H 2 O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A 440 ) using a SpectraMax 190. Net A 440  was calculated by subtracting the A 440  of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of triplicate assays. The proteolytic activity is shown as Net A 440 . The proteolytic assay with azo-casein as the substrate (shown in  FIG. 4.2 ) indicates that PehPro1 is an active protease. 
     Example 4.4 
     pH Profile of Metalloprotease PehPro1 
     With azo-casein as the substrate, the pH profile of metalloprotease PehPro1 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl Milli-Q H 2 O diluted enzyme (250 ppm) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 4.3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in  FIG. 4.3 , the optimal pH of PehPro1 is 7, with greater than 70% of maximal activity retained between 5.5 and 9.5. 
     Example 4.5 
     Temperature Profile of Metalloprotease PehPro1 
     The temperature profile of metalloprotease PehPro1 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assays. The enzyme sample and azo-casein substrate were prepared as in Example 4.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H 2 O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20-90° C.) for 5 min. After the incubation, 5 μl of diluted enzyme (100 ppm) or H 2 O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 4.3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in  FIG. 4.4  suggest that PehPro1 showed an optimal temperature at 70° C., and retained greater than 70% of its maximum activity between 60 and 75° C. 
     Example 4.6 
     Cleaning Performance of Metalloprotease PehPro1 
     The cleaning performance of PehPro1 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified protease samples were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl 2 , 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca 2+ :Mg 2+ =3:1) (detergent composition shown in Table 4.1). To initiate the reaction, 180 μl of the AT detergent buffered at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the “Initial performance”) using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as the “Wash-off”). The sum of two absorbance measurements (“Initial performance” plus “Wash-off”) gives the “Total performance”, which measures the protease activity on the model stain; and Net A 405  was subsequently calculated by subtracting the A 405  of the “Total performance” of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 for PehPro1 in AT detergent is shown in  FIGS. 4.5A and 4.5B . 
     
       
         
           
               
             
               
                 TABLE 4.1 
               
             
            
               
                   
               
               
                 Composition of AT dish detergent 
               
            
           
           
               
               
            
               
                 Ingredient 
                 Concentration (mg/ml) 
               
               
                   
               
            
           
           
               
               
            
               
                 MGDA (methylglycinediacetic acid) 
                 0.143 
               
               
                 Sodium citrate 
                 1.86 
               
               
                 Citric acid* 
                 varies 
               
               
                 Plurafac ® LF 18B (a non-ionic surfactant) 
                 0.029 
               
               
                 Bismuthcitrate 
                 0.006 
               
               
                 Bayhibit ® S  
                 0.006 
               
               
                 (Phosphonobutantricarboxylic acid sodium salt) 
                   
               
               
                 Acusol ™ 587 (a calcium polyphosphate  
                 0.029 
               
               
                 inhibitor) 
                   
               
               
                 PEG 6000 
                 0.043 
               
               
                 PEG 1500 
                 0.1 
               
               
                   
               
               
                 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid. 
               
            
           
         
       
     
     Example 4.7 
     Comparison of PehPro1 to Other Proteases 
     A. Identification of Homologous Proteases 
     Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The mature protein amino acid sequence for PehPro1 (SEQ ID NO: 18) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 4.2A and 4.2B provide a list of sequences with the percent identity to PehPro1. The length in Table 4.2 refers to the entire sequence length of the homologous proteases. 
     
       
         
           
               
             
               
                 TABLE 4.2A 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PehPro1 protein identified from the 
               
               
                 NCBI non-redundant protein database 
               
            
           
           
               
               
               
               
            
               
                 Accession # 
                 PID to PehPro1 
                 Organism 
                 Length 
               
               
                   
               
               
                 ZP_09077634.1 
                 88 
                   Paenibacillus elgii  B69 
                 524 
               
               
                 ZP_09071078.1 
                 74 
                   Paenibacillus larvae  subsp.  larvae  B-3650 
                 529 
               
               
                 YP_003872179.1 
                 74 
                   Paenibacillus polymyxa  E681 
                 592 
               
               
                 P29148 
                 73 
                 
                   Paenibacillus polymyxa 
                 
                 590 
               
               
                 P43263 
                 68 
                 
                   Brevibacillus brevis 
                 
                 527 
               
               
                 ZP_09775365.1 
                 68 
                   Paenibacillus  sp. Aloe-11 
                 580 
               
               
                 ZP_10241029.1 
                 67 
                   Paenibacillus peoriae  KCTC 3763 
                 599 
               
               
                 ZP_10575942.1 
                 66 
                   Brevibacillus  sp. BC25 
                 528 
               
               
                 YP_002770810.1 
                 67 
                   Brevibacillus brevis  NBRC 100599 
                 528 
               
               
                 ZP_08640523.1 
                 64 
                   Brevibacillus laterosporus  LMG 15441 
                 564 
               
               
                 YP_004646155.1 
                 63 
                   Paenibacillus mucilaginosus  KNP414 
                 525 
               
               
                 ZP_08093424.1 
                 60 
                   Planococcus donghaensis  MPA1U2 
                 553 
               
               
                 YP_003670279.1 
                 59 
                   Geobacillus  sp. C56-T3 
                 546 
               
               
                 P00800 
                 59 
                 
                   Bacillus thermoproteolyticus 
                 
                 548 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4.2B 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PehPro1 protein 
               
               
                 identified from the Genome Quest Patent database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Patent ID # 
                 PehPro1 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 JP2005229807-0019 
                 74.5 
                 
                   Paenibacillus polymyxa 
                 
                 566 
               
               
                 US20120107907-0187 
                 74.09 
                 
                   Bacillus polymyxa 
                 
                 302 
               
               
                 US8114656-0186 
                 68.21 
                 
                   Bacillus brevis 
                 
                 304 
               
               
                 WO2004011619-0044 
                 63.25 
                 empty 
                 507 
               
               
                 EP2390321-0185 
                 62.9 
                 
                   Bacillus cereus 
                 
                 317 
               
               
                 WO2012110563-0004 
                 62.7 
                 
                   Bacillus megaterium 
                 
                 320 
               
               
                 WO2012110563-0005 
                 62.58 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 JP1995184649-0001 
                 62.5 
                   Lactobacillus  sp. 
                 566 
               
               
                 JP2005333991-0002 
                 62.38 
                 empty 
                 562 
               
               
                 EP2178896-0184 
                 62.18 
                 
                   Bacillus anthracis 
                 
                 566 
               
               
                 JP1994014788-0003 
                 61.94 
                 empty 
                 317 
               
               
                 EP2390321-0178 
                 61.86 
                 
                   Bacillus thuringiensis 
                 
                 566 
               
               
                 US6518054-0002 
                 60.84 
                   Bacillus  sp. 
                 316 
               
               
                 US8114656-0176 
                 60.13 
                 
                   Bacillus stearothermophilus 
                 
                 548 
               
               
                 US6103512-0003 
                 59.81 
                 empty 
                 319 
               
               
                 US20120107907-0184 
                 59.49 
                 
                   Bacillus caldoyticus 
                 
                 319 
               
               
                   
               
            
           
         
       
     
     B. Alignment of Homologous Protease Sequences 
     The amino acid sequence of predicted mature PehPro1 (SEQ ID NO: 18) was aligned with thermolysin (P00800,  Bacillus thermoproteolyticus ) and protease from  Paenibacillus elgii B 69 (ZP_09077634.1) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters.  FIG. 4.6  shows the alignment of PehPro1 with these protease sequences. 
     C. Phylogenetic Tree 
     A phylogenetic tree for precursor protein PehPro1 (SEQ ID NO: 17) was built using sequences of representative homologs from Table 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. MolBiol.Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in  FIG. 4.7 . 
     Example 5.1 
     Cloning of  Paenibacillus barcinonensis  Metalloprotease PbaPro1 
     A strain (DSM15478) of  Paenibacillus barcinonensis  was selected as a potential source of enzymes which may be useful in various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the  Paenibacillus barcinonensis  strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina&#39;s next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in  Paenibacillus barcinonensis  encodes a metalloprotease and the sequence of this gene, called PbaPro1, is provided in SEQ ID NO: 21. The corresponding protein encoded by the PbaPro1 gene is shown in SEQ ID NO: 22. At the N-terminus, the protein has a signal peptide with a length of 25 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PbaPro1 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the  Paenibacillus polymyxa  Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PbaPro1 protein is shown in SEQ ID NO: 23. 
     The nucleotide sequence of the PbaPro1 gene isolated from  Paenibacillus barcinonensis  is set forth as SEQ ID NO: 21. The sequence encoding the predicted native signal peptide is shown in italics: 
     
       
         
           
               
            
               
                 
                   ATGAAATTGACCAAAATTATGCCAACAATTCTTGCAGGAGCTCTTTT 
                 
               
               
                   
               
               
                   GCTCACATCCCTGTCCTCTGCAGCAGCA ATGCCGTTATCTGACTCAT 
               
               
                   
               
               
                 CCATTCCATTTGAGGGCCCCTACACCTCCGAGGAGAGTATTCTGTTG 
               
               
                   
               
               
                 AACAACAACCCGGACGAAATGATTTATAATTTTCTTGCACAACAAGA 
               
               
                   
               
               
                 GCAATTTCTGAATGCCGACGTCAAAGGACAGCTCAAAATCATTAAAC 
               
               
                   
               
               
                 GCAACACAGACACTTCCGGCATCAGACACTTTCGTCTGAAGCAATAC 
               
               
                   
               
               
                 ATCAAAGGTGTTCCGGTTTACGGCGCAGAACAAACGATCCATCTGGA 
               
               
                   
               
               
                 CAAGAACGGAGCTGTAACTTCCGCACTCGGCGATCTTCCGCCAATTG 
               
               
                   
               
               
                 AAGAACAGGCTGTTCCGAATGATGGCGTTCCCGCAATCAGTGCAGAC 
               
               
                   
               
               
                 GATGCCATCCGTGCCGCCGAGAATGAAGCCACCTCCCGTCTTGGAGA 
               
               
                   
               
               
                 GCTTGGCGCACCAGAGCTTGAGCCAAAGGCCGAATTAAACATTTATC 
               
               
                   
               
               
                 ATCATGAAGATGACGGACAAACCTACCTCGTTTACATTACGGAAGTT 
               
               
                   
               
               
                 AACGTGCTTGAGCCTTCCCCGCTACGGACCAAATATTTTATTAACGC 
               
               
                   
               
               
                 CCTTGATGGAAGCATCGTATCTCAATACGATATTATCAACTTTGCCA 
               
               
                   
               
               
                 CAGGCACCGGTACAGGCGTGCATGGTGATACCAAAACACTGACGACA 
               
               
                   
               
               
                 ACTCAATCCGGCAGCACCTATCAGCTGAAAGATACAACTCGTGGAAA 
               
               
                   
               
               
                 AGGCATTCAAACCTATACTGCGAACAATCGCTCCTCGCTTCCAGGCA 
               
               
                   
               
               
                 GCTTGTCTACCAGTTCCAATAACGTATGGACAGACCGTGCAGCTGTA 
               
               
                   
               
               
                 GATGCGCACGCCTATGCTGCCGCCACATATGACTTCTACAAAAACAA 
               
               
                   
               
               
                 ATTCAATCGCAACGGCATTGACGGAAACGGGCTGTTGATTCGCTCTA 
               
               
                   
               
               
                 CAGTGCATTATGGCTCCAACTATAAAAACGCCTTCTGGAACGGAGCA 
               
               
                   
               
               
                 CAGATTGTCTATGGAGATGGCGATGGCATCGAGTTCGGTCCCTTCTC 
               
               
                   
               
               
                 CGGTGATCTCGATGTTGTCGGACATGAATTGACACACGGGGTGATTG 
               
               
                   
               
               
                 AATATACAGCCAATCTCGAATATCGCAATGAGCCGGGTGCTTTAAAC 
               
               
                   
               
               
                 GAAGCTTTTGCCGACATTATGGGGAACACCATCGAAAGCAAAAACTG 
               
               
                   
               
               
                 GCTGCTTGGCGACGGAATCTATACTCCAAACATTCCAGGTGATGCCC 
               
               
                   
               
               
                 TGCGCTCGTTATCCGACCCTACGCTGTATAACCAGCCTGACAAATAC 
               
               
                   
               
               
                 AGTGATCGCTACACTGGCTCTCAGGATAATGGCGGTGTGCATATCAA 
               
               
                   
               
               
                 CAGCGGGATCATTAACAAAGCATATTATCTTGCAGCCCAAGGCGGTA 
               
               
                   
               
               
                 CTCATAACGGGGTAACCGTTAGCGGCATCGGCCGGGATAAAGCAGTA 
               
               
                   
               
               
                 CGTATTTTCTATAGCACGCTGGTGAACTACCTGACGCCAACCTCCAA 
               
               
                   
               
               
                 ATTTGCAGCAGCCAAAACAGCGACAATTCAGGCAGCCAAGGACCTGT 
               
               
                   
               
               
                 ACGGTGCCAATTCCGCTGAAGCTACGGCAATCACCAAAGCTTATCAA 
               
               
                   
               
               
                 GCGGTAGGTTTG 
               
            
           
         
       
     
     The amino acid sequence of the PbaPro1 precursor protein is set forth as SEQ ID NO: 22. The predicted signal sequence is shown in italics, and the predicted pro-peptide is shown in underlined text: 
     
       
         
           
               
            
               
                 
                   MKLTKIMPTILAGALLLTSLSSAAA 
                   MPLSDSSIPFEGPYTSEESILL 
                 
               
               
                   
               
               
                 
                   NNNPDEMIYNFLAQQEQFLNADVKGQLKIIKRNTDTSGIRHFRLKQY 
                 
               
               
                   
               
               
                 
                   IKGVPVYGAEQTIHLDKNGAVTSALGDLPPIEEQAVPNDGVPAISAD 
                 
               
               
                   
               
               
                 
                   DAIRAAENEATSRLGELGAPELEPKAELNIYHHEDDGQTYLVYITEV 
                 
               
               
                   
               
               
                   NVLEPSPLRTKYFINALDGSIVSQYDIINF ATGTGTGVHGDTKTLTT 
               
               
                   
               
               
                 TQSGSTYQLKDTTRGKGIQTYTANNRSSLPGSLSTSSNNVWTDRAAV 
               
               
                   
               
               
                 DAHAYAAATYDFYKNKFNRNGIDGNGLLIRSTVHYGSNYKNAFWNGA 
               
               
                   
               
               
                 QIVYGDGDGIEFGPFSGDLDVVGHELTHGVIEYTANLEYRNEPGALN 
               
               
                   
               
               
                 EAFADIMGNTIESKNWLLGDGIYTPNIPGDALRSLSDPTLYNQPDKY 
               
               
                   
               
               
                 SDRYTGSQDNGGVHINSGIINKAYYLAAQGGTHNGVTVSGIGRDKAV 
               
               
                   
               
               
                 RIFYSTLVNYLTPTSKFAAAKTATIQAAKDLYGANSAEATAITKAYQ 
               
               
                   
               
               
                 AVGL 
               
            
           
         
       
     
     The amino acid sequence of the predicted mature form of PbaPro1 is set forth as SEQ ID NO: 23: 
     
       
         
           
               
            
               
                 ATGTGTGVHGDTKTLTTTQSGSTYQLKDTTRGKGIQTYTANNRSSLP 
               
               
                   
               
               
                 GSLSTSSNNVWTDRAAVDAHAYAAATYDFYKNKFNRNGIDGNGLLIR 
               
               
                   
               
               
                 STVHYGSNYKNAFWNGAQIVYGDGDGIEFGPFSGDLDVVGHELTHGV 
               
               
                   
               
               
                 IEYTANLEYRNEPGALNEAFADIMGNTIESKNWLLGDGIYTPNIPGD 
               
               
                   
               
               
                 ALRSLSDPTLYNQPDKYSDRYTGSQDNGGVHINSGIINKAYYLAAQG 
               
               
                   
               
               
                 GTHNGVTVSGIGRDKAVRIFYSTLVNYLTPTSKFAAAKTATIQAAKD 
               
               
                   
               
               
                 LYGANSAEATAITKAYQAVGL 
               
            
           
         
       
     
     Example 5.2 
     Expression of  Paenibacillus barcinonensis  Metalloprotease PbaPro1 
     The DNA sequence of the propeptide-mature form of PbaPro1 was synthesized and inserted into the  Bacillus subtilis  expression vector p2JM103BBI (Vogtentanz,  Protein Expr Purif,  55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX147(AprE-PbaPro1) ( FIG. 5.1 ). Ligation of this gene encoding the PbaPro1 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3′ end of the  B. subtilis  AprE signal sequence and the 5′ end of the predicted PbaPro1 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in  FIG. 1  was labeled pGX147(AprE-PbaPro1). As shown in  FIG. 5.1 , pGX147(AprE-PbaPro1) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in  B. subtilis , and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PbaPro1 (SEQ ID NO: 24). The translation product of the synthetic AprE-PbaPro1 gene is shown in SEQ ID NO: 25. 
     The pGX147(AprE-PbaPro1) plasmid was then transformed into  B. subtilis  cells (degU Hy  32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat #232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium. supplemented with additional 5 mM CaCl 2 ). 
     The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl 2  and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to an 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above; and the active flow-through fractions were collected and concentrated. The sample was loaded onto a 320 ml Superdex 75 gel filtration column pre-equilibrated with the loading buffer described above containing 0.15 M NaCl. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses. 
     The nucleotide sequence of the synthesized PbaPro1 gene in plasmid pGX147(AprE-PbaPro1) is depicted in SEQ ID NO: 24. The sequence encoding the three residue addition (AGK) is shown in bold: 
     
       
         
           
               
            
               
                 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTT 
               
               
                   
               
               
                 AATCTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCT GCTGGAA   
               
               
                   
               
               
                   AA ATGCCTCTGTCAGACAGCAGCATTCCGTTTGAGGGCCCGTACACA 
               
               
                   
               
               
                 TCAGAAGAAAGCATCCTGCTGAACAACAACCCGGACGAGATGATCTA 
               
               
                   
               
               
                 CAATTTCCTGGCACAGCAGGAGCAGTTCCTGAACGCAGACGTGAAGG 
               
               
                   
               
               
                 GCCAGCTGAAAATCATCAAAAGAAACACAGACACGAGCGGCATCAGA 
               
               
                   
               
               
                 CACTTCAGACTGAAGCAGTACATCAAGGGCGTCCCGGTTTACGGCGC 
               
               
                   
               
               
                 TGAGCAGACAATCCACCTGGACAAAAATGGCGCAGTGACGAGCGCAC 
               
               
                   
               
               
                 TTGGAGATCTGCCGCCGATTGAAGAGCAAGCAGTCCCGAACGATGGC 
               
               
                   
               
               
                 GTTCCGGCGATTAGCGCTGATGACGCTATCAGAGCCGCGGAAAACGA 
               
               
                   
               
               
                 AGCGACGTCAAGACTGGGAGAACTTGGCGCACCGGAACTTGAACCGA 
               
               
                   
               
               
                 AGGCGGAACTGAACATCTATCACCACGAAGACGATGGACAGACGTAC 
               
               
                   
               
               
                 CTGGTGTACATCACGGAGGTGAATGTGCTGGAGCCGTCACCGCTGAG 
               
               
                   
               
               
                 AACAAAATACTTCATCAATGCGCTGGATGGCAGCATCGTTAGCCAAT 
               
               
                   
               
               
                 ACGACATCATTAACTTCGCCACAGGCACGGGCACAGGCGTTCATGGC 
               
               
                   
               
               
                 GACACAAAAACGCTTACGACAACACAGTCAGGCTCAACGTACCAGCT 
               
               
                   
               
               
                 GAAAGACACAACAAGAGGCAAGGGCATCCAGACGTATACAGCCAATA 
               
               
                   
               
               
                 ACAGAAGCTCACTTCCGGGCTCACTGTCAACAAGCAGCAATAATGTC 
               
               
                   
               
               
                 TGGACGGACAGAGCTGCAGTGGACGCGCACGCGTATGCTGCGGCCAC 
               
               
                   
               
               
                 GTACGACTTCTACAAGAACAAGTTCAACAGAAACGGCATTGATGGCA 
               
               
                   
               
               
                 ACGGCCTGCTTATTAGAAGCACGGTCCACTACGGCTCAAACTACAAG 
               
               
                   
               
               
                 AATGCGTTTTGGAACGGCGCCCAAATTGTTTATGGCGATGGAGACGG 
               
               
                   
               
               
                 CATCGAGTTCGGACCTTTTAGCGGCGACCTGGATGTGGTCGGACATG 
               
               
                   
               
               
                 AACTGACGCACGGCGTTATCGAGTATACGGCGAATCTGGAATACAGA 
               
               
                   
               
               
                 AATGAACCGGGCGCTCTGAATGAGGCCTTCGCGGATATCATGGGCAA 
               
               
                   
               
               
                 CACAATTGAGAGCAAAAACTGGCTTCTGGGCGACGGAATCTACACGC 
               
               
                   
               
               
                 CGAACATTCCGGGAGATGCACTGAGATCACTGAGCGACCCTACGCTG 
               
               
                   
               
               
                 TACAACCAGCCGGACAAATACAGCGACAGATACACGGGATCACAGGA 
               
               
                   
               
               
                 CAATGGCGGCGTCCATATTAACTCAGGCATCATCAACAAAGCGTATT 
               
               
                   
               
               
                 ATCTGGCAGCTCAAGGCGGCACGCATAATGGCGTCACAGTTAGCGGA 
               
               
                   
               
               
                 ATCGGCAGAGACAAGGCCGTCAGAATTTTCTACTCAACGCTGGTGAA 
               
               
                   
               
               
                 CTACCTGACACCGACAAGCAAGTTTGCAGCCGCCAAAACAGCCACGA 
               
               
                   
               
               
                 TTCAGGCAGCAAAGGACCTGTACGGAGCGAACTCAGCAGAGGCCACA 
               
               
                   
               
               
                 GCGATTACGAAGGCTTATCAAGCCGTGGGACTGTAA 
               
            
           
         
       
     
     The amino acid sequence of the PbaPro1 precursor protein expressed from plasmid pGX147(AprE-PbaPro1) is depicted in SEQ ID NO: 25. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text. 
     
       
         
           
               
            
               
                 
                   MRSKKLWISLLFALTLIFTMAFSNMSAQA 
                   AGK 
                   MPLSDSSIPFEGPYT 
                 
               
               
                   
               
               
                 
                   SEESILLNNNPDEMIYNFLAQQEQFLNADVKGQLKIIKRNTDTSGIR 
                 
               
               
                   
               
               
                 
                   HFRLKQYIKGVPVYGAEQTIHLDKNGAVTSALGDLPPIEEQAVPNDG 
                 
               
               
                   
               
               
                 
                   VPAISADDAIRAAENEATSRLGELGAPELEPKAELNIYHHEDDGQTY 
                 
               
               
                   
               
               
                   LVYITEVNVLEPSPLRTKYFINALDGSIVSQYDIINF ATGTGTGVHG 
               
               
                   
               
               
                 DTKTLTTTQSGSTYQLKDTTRGKGIQTYTANNRSSLPGSLSTSSNNV 
               
               
                   
               
               
                 WTDRAAVDAHAYAAATYDFYKNKFNRNGIDGNGLLIRSTVHYGSNYK 
               
               
                   
               
               
                 NAFWNGAQIVYGDGDGIEFGPFSGDLDVVGHELTHGVIEYTANLEYR 
               
               
                   
               
               
                 NEPGALNEAFADIMGNTIESKNWLLGDGIYTPNIPGDALRSLSDPTL 
               
               
                   
               
               
                 YNQPDKYSDRYTGSQDNGGVHINSGIINKAYYLAAQGGTHNGVTVSG 
               
               
                   
               
               
                 IGRDKAVRIFYSTLVNYLTPTSKFAAAKTATIQAAKDLYGANSAEAT 
               
               
                   
               
               
                 AITKAYQAVGL 
               
            
           
         
       
     
     Example 5.3 
     Proteolytic Activity of Metalloprotease PbaPro1 
     The proteolytic activity of purified metalloprotease PbaPro1 was measured in 50 mM Tris (pH 7), using azo-casein (Cat #74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H 2 O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A 440 ) using a SpectraMax 190. Net A 440  was calculated by subtracting the A 440  of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of triplicate assays. 
     The proteolytic activities are shown as Net A 44 o. The proteolytic assay with azo-casein as the substrate (shown in  FIG. 5.2 ) indicates that PbaPro1 is an active protease. 
     Example 5.4 
     pH Profile of Metalloprotease PbaPro1 
     With azo-casein as the substrate, the pH profile of metalloprotease PbaPro1 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 5 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl Milli-Q H 2 O diluted enzyme (125 ppm) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 5.3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in  FIG. 5.3 , the optimal pH of PbaPro1 is 8, with greater than 70% of maximal activity retained between 7 and 9. 
     Example 5.5 
     Temperature Profile of Metalloprotease PbaPro1 
     The temperature profiles of metalloprotease PbaPro1 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assays. The enzyme sample and azo-casein substrate were prepared as in Example 5.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H 2 O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20-90° C.) for 5 min. After the incubation, 5 μl of diluted enzyme (50 ppm) or H 2 O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 5.3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in  FIG. 5.4  suggest that PbaPro1 showed an optimal temperature at 50° C., and retained greater than 70% of its maximum activity between 45 and 55° C. 
     Example 5.6 
     Cleaning Performance of Metalloprotease PbaPro1 
     The cleaning performance of PbaPro1 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified protease samples were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl 2 , 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca 2+ :Mg 2+ =3:1) (detergent composition shown in Table 5.1). To initiate the reaction, 180 μl of the AT detergent buffered at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the “Initial performance”) using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as the “Wash-off”). The sum of two absorbance measurements (“Initial performance” plus “Wash-off”) gives the “Total performance”, which measures the protease activity on the model stain; and Net A 405  was subsequently calculated by subtracting the A 405  of the “Total performance” of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 in AT detergent for PbaPro1 is shown in  FIGS. 5.5A and 5.5B . 
     
       
         
           
               
             
               
                 TABLE 5.1 
               
             
            
               
                   
               
               
                 Composition of AT dish detergent formula with bleach 
               
            
           
           
               
               
            
               
                   
                 Concentration  
               
               
                 Ingredient 
                 (mg/ml) 
               
               
                   
               
            
           
           
               
               
            
               
                 MGDA (methylglycinediacetic acid) 
                 0.143 
               
               
                 Sodium citrate 
                 1.86 
               
               
                 Citric acid* 
                 varies 
               
               
                 PAP (peracid N,N-phthaloylaminoperoxycaproic acid) 
                 0.057 
               
               
                 Plurafac ® LF 18B (a non-ionic surfactant) 
                 0.029 
               
               
                 Bismuthcitrate 
                 0.006 
               
               
                 Bayhibit ® S (Phosphonobutantricarboxylic  
                 0.006 
               
               
                 acid sodium salt) 
                   
               
               
                 Acusol ™ 587 (a calcium polyphosphate inhibitor) 
                 0.029 
               
               
                 PEG 6000 
                 0.043 
               
               
                 PEG 1500 
                 0.1 
               
               
                   
               
               
                 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid. 
               
            
           
         
       
     
     Example 5.7 
     Comparison of PbaPro1 to Other Proteases 
     A. Identification of Homologous Proteases 
     Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The predicted mature protein amino acid sequence for PbaPro1 (SEQ ID NO: 23) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 5.2A and 5.2B provide a list of sequences with the percent identity to PbaPro1. The length in Table 5.2 refers to the entire sequence length of the homologous proteases. 
     
       
         
           
               
             
               
                 TABLE 5.2A 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PbaPro1 protein 
               
               
                 identified from the NCBI non-redundant protein database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Accession # 
                 PbaPro1 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 AAB02774.1 
                 56 
                 
                   Geobacillus stearothermophilus 
                 
                 552 
               
               
                 P00800 
                 56 
                 
                   Bacillus stermoproteolyticus 
                 
                 548 
               
               
                 AAA22623.1 
                 57 
                 
                   Bacillus caldolyticus 
                 
                 544 
               
               
                 YP_003670279.1 
                 57 
                   Geobacillus  sp. C56-T3 
                 546 
               
               
                 AAC43402.1 
                 57 
                 
                   Alicyclobacillus acidocaldarius 
                 
                 546 
               
               
                 YP_003597483.1 
                 57 
                   Bacillus megaterium  DSM 319 
                 562 
               
               
                 ZP_08093424.1 
                 57 
                   Planococcus donghaensis  MPA1U2 
                 553 
               
               
                 ZP_08640523.1 
                 59 
                 
                   Brevibacillus laterosporus 
                 
                 564 
               
               
                   
                   
                 LMG 15441 
                   
               
               
                 ZP_04216147.1 
                 59 
                   Bacillus cereus  Rock3-44 
                 566 
               
               
                 YP_001373863.1 
                 60 
                   Bacillus cytotoxicus  NVH 391-98 
                 565 
               
               
                 YP_004646155.1 
                 60 
                 
                   Paenibacillus mucilaginosus 
                 
                 525 
               
               
                   
                   
                 KNP414 
                   
               
               
                 ZP_10738945.1 
                 61 
                   Brevibacillus  sp. CF112 
                 528 
               
               
                 CAA43589.1 
                 63 
                 
                   Brevibacillus brevis 
                 
                 527 
               
               
                 ZP_02326602.1 
                 64 
                   Paenibacillus larvae  subsp. 
                 520 
               
               
                   
                   
                   larvae  BRL-230010 
                   
               
               
                 ZP_02326503.1 
                 65 
                   Paenibacillus larvae  subsp.  larvae   
                 520 
               
               
                   
                   
                 B-3650 
                   
               
               
                 ZP_09077634.1 
                 66 
                   Paenibacillus elgii  B69 
                 524 
               
               
                 ZP_08511445.1 
                 68 
                   Paenibacillus  sp. HGF7 
                 525 
               
               
                 ZP_09775364.1 
                 70 
                   Paenibacillus  sp. Aloe-11 
                 593 
               
               
                 YP_005073223.1 
                 70 
                   Paenibacillus terrae  HPL-003 
                 591 
               
               
                 ZP_10241030.1 
                 70 
                   Paenibacillus peoriae  KCTC 3763 
                 593 
               
               
                 YP_003948511.11 
                 71 
                   Paenibacillus polymyxa  SC2 
                 592 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5.2B 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PbaPro1 protein 
               
               
                 identified from the Genome Quest Patent database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Patent # 
                 PbaPro1 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 JP2005333991-0002 
                 56.91 
                   
                 562 
               
               
                 WO2012110562-0007 
                 56.96 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 WO2012110562-0006 
                 57.23 
                 
                   Bacillus megaterium 
                 
                 320 
               
               
                 EP2390321-0178 
                 57.23 
                 
                   Bacillus thuringiensis 
                 
                 566 
               
               
                 EP2390321-0184 
                 57.56 
                 
                   Bacillus caldoyticus 
                 
                 319 
               
               
                 WO2007044993-0184 
                 57.56 
                   Bacillus  sp. 
                 319 
               
               
                 US20120107907-0177 
                 57.56 
                 
                   Bacillus caldolyticus 
                 
                 544 
               
               
                 CN102168095-0002 
                 57.88 
                   
                 319 
               
               
                 WO2012110562-0004 
                 57.88 
                 
                   Bacillus caldolyticus 
                 
                 319 
               
               
                 WO2012110562-0003 
                 57.88 
                 
                   Geobacillus stearothermophilus 
                 
                 319 
               
               
                 WO2004011619-0056 
                 57.88 
                   
                 546 
               
               
                 JP1995184649-0001 
                 57.88 
                   Lactobacillus  sp. 
                 566 
               
               
                 JP2010535248-0240 
                 57.88 
                 
                   Bacillus anthracis 
                 
                 566 
               
               
                 US6518054-0001 
                 58.2 
                   Bacillus  sp. 
                 319 
               
               
                 US6103512-0003 
                 58.2 
                   
                 319 
               
               
                 WO2011163237-0001 
                 58.2 
                 
                   Geobacillus stearothermophilus 
                 
                 548 
               
               
                 JP1994014788-0003 
                 58.25 
                   
                 317 
               
               
                 US8114656-0185 
                 58.9 
                 
                   Bacillus cereus 
                 
                 317 
               
               
                 US20120107907-0179 
                 58.9 
                 
                   Bacillus cereus 
                 
                 566 
               
               
                 WO2012110563-0005 
                 59.22 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 WO2004011619-0044 
                 59.6 
                   
                 507 
               
               
                 US20120107907-0186 
                 63.25 
                 
                   Bacillus brevis 
                 
                 304 
               
               
                 JP2005229807-0018 
                 70.86 
                 
                   Paenibacillus polymyxa 
                 
                 566 
               
               
                 EP2390321-0187 
                 71.1 
                 
                   Bacillus polymyxa 
                 
                 302 
               
               
                 JP2009511072-0203 
                 71.1 
                 
                   Paenibacillus polymyxa 
                 
                 302 
               
               
                   
               
            
           
         
       
     
     B. Alignment of Homologous Protease Sequences 
     The amino acid sequence of the predicted mature PbaPro1 (SEQ ID NO: 23) was aligned with Thermolysin (P00800,  Bacillus thermoproteolyticus ), and protease from  Paenibacillus polymyxa  SC2 (YP 003948511.1) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters.  FIG. 5.6  shows the alignment of PbaPro1 with these protease sequences. 
     C. Phylogenetic Tree 
     A phylogenetic tree for full length sequence of PbaPro1 (SEQ ID NO: 22) was built using sequences of representative homologs from Table 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. MolBiol.Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in  FIG. 5.7 . 
     Example 6.1 
     Cloning of  Paenibacillus polymyxa  SC2 Metalloprotease PpoPro1 
     The nucleic acid sequence for the PpoPro1 gene was identified in the NCBI database (NCBI Reference Sequence: NC 014622.1 from 4536397-4538175) and is provided in SEQ ID NO: 26. The corresponding protein encoded by the PpoPro1 gene is shown in SEQ ID NO: 27. At the N-terminus, the protein has a signal peptide with a length of 24 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PpoPro1 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the  Paenibacillus polymyxa  Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PpoPro1 protein is shown in SEQ ID NO: 28. 
     The nucleotide sequence of the PpoPro1 gene identified from NCBI database is set forth as SEQ ID NO: 26. The sequence encoding the predicted native signal peptide is shown in italics: 
     
       
         
           
               
            
               
                 
                   ATGAAAAAAGTATGGGTTTCGCTTCTTGGAGGAGCTATGTTATTAGGGTCT 
                 
               
               
                   
               
               
                   GTCGCGTCTGGTGCATCTGCG GAGAGTTCCGTTTCGGGGCCAGCTCAGCTT 
               
               
                   
               
               
                 ACACCGACCTTCCACGCCGAGCAATGGAAAGCACCTACCTCGGTATCGGGG 
               
               
                   
               
               
                 GATGACATTGTATGGAGCTATTTAAATCGACAAAAGAAATCGTTGCTGGGT 
               
               
                   
               
               
                 GTGGATAGCTCCAGTGTACGTGAACAATTCCGAATCGTTGATCGCACAAGC 
               
               
                   
               
               
                 GACAAATCCGGTGTAAGCCATTATCGACTGAAGCAGTATGTAAACGGAATT 
               
               
                   
               
               
                 CCCGTGTATGGAGCTGAACAAACTATTCATGTGGGCAAATCTGGTGAGGTC 
               
               
                   
               
               
                 ACCTCTTACTTAGGAGCGGTGGTTAATGAGGATCAGCAGGCAGAAGCTACG 
               
               
                   
               
               
                 CAAGGTACAACTCCAAAAATCAGCGCTTCTGAAGCGGTCTACACCGCATAT 
               
               
                   
               
               
                 AAAGAAGCAGCTGCACGGATTGAAGCCCTCCCTACCTCCGACGATACTATT 
               
               
                   
               
               
                 TCTAAAGACGCTGAGGAGCCAAGCAGTGTAAGTAAAGATACTTACGCCGAA 
               
               
                   
               
               
                 GCAGCTAACAACGAAAAAACGCTTTCTGTTGATAAGGACGAGCTGAGTCTT 
               
               
                   
               
               
                 GATCAGGCATCTGTCCTGAAAGATAGCAAAATTGAAGCAGTGGAACCAGAA 
               
               
                   
               
               
                 AAAAGTTCCATTGCCAAAATCGCTAATCTGCAGCCTGAAGTAGATCCTAAA 
               
               
                   
               
               
                 GCAGAACTCTACTACTACCCTAAGGGGGATGACCTGCTGCTGGTTTATGTA 
               
               
                   
               
               
                 ACAGAAGTTAATGTTTTAGAACCTGCCCCACTGCGTACCCGCTACATTATT 
               
               
                   
               
               
                 GATGCCAATGACGGCAGCATCGTATTCCAGTATGACATCATTAATGAAGCG 
               
               
                   
               
               
                 ACAGGCACAGGTAAAGGTGTGCTTGGTGATTCCAAATCGTTCACTACTACC 
               
               
                   
               
               
                 GCTTCCGGCAGTAGCTACCAGTTAAAAGATACAACACGCGGTAACGGAATC 
               
               
                   
               
               
                 GTGACTTACACGGCCTCCAACCGTCAAAGCATCCCAGGTACCATTTTGACA 
               
               
                   
               
               
                 GATGCCGATAATGTATGGAATGATCCAGCTGGTGTGGACGCCCATGCGTAT 
               
               
                   
               
               
                 GCTGCTAAAACCTATGATTACTATAAAGCCAAATTTGGACGCAACAGCATT 
               
               
                   
               
               
                 GACGGACGCGGTCTGCAACTTCGTTCGACGGTCCATTACGGTAGTCGCTAC 
               
               
                   
               
               
                 AACAATGCCTTCTGGAACGGCTCCCAAATGACTTATGGAGATGGAGATGGT 
               
               
                   
               
               
                 AGCACATTTATCGCCTTCAGCGGGGACCCCGATGTAGTAGGACATGAACTT 
               
               
                   
               
               
                 ACGCATGGTGTCACAGAGTATACTTCGAATTTGGAATATTACGGAGAGTCC 
               
               
                   
               
               
                 GGCGCATTGAATGAAGCTTTCTCAGACGTTATCGGGAATGACATTCAGCGC 
               
               
                   
               
               
                 AAAAACTGGCTTGTAGGCGATGATATTTACACGCCAAACATTGCAGGCGAT 
               
               
                   
               
               
                 GCCCTTCGCTCAATGTCCAATCCAACCCTGTACGATCAACCAGATCACTAT 
               
               
                   
               
               
                 TCCAACCTGTACAGAGGCAGCTCCGATAACGGCGGTGTTCACACCAACAGC 
               
               
                   
               
               
                 GGTATTATCAATAAAGCTTACTACTTGTTAGCACAAGGTGGTAATTTCCAT 
               
               
                   
               
               
                 GGCGTAACTGTAAATGGAATTGGCCGTGATGCAGCGGTGCAAATTTACTAC 
               
               
                   
               
               
                 AGTGCCTTTACGAACTACCTGACTTCTTCTTCCGACTTCTCCAACGCACGT 
               
               
                   
               
               
                 GCTGCTGTGATCCAAGCCGCAAAAGATCTGTACGGGGCGAACTCAGCAGAA 
               
               
                   
               
               
                 GCAACTGCAGCTGCCAAGTCTTTTGACGCTGTAGGCGTAAACTAA 
               
            
           
         
       
     
     The amino acid sequence of the PpoPro1 precursor protein is set forth as SEQ ID NO: 27. The predicted signal sequence is shown in italics, and the predicted propeptide is shown in underlined text: 
     
       
         
           
               
            
               
                 
                   MKKVWVSLLGGAMLLGSVASGASA 
                   ESSVSGPAQLTPTFHAEQWKAPTSVSG 
                 
               
               
                   
               
               
                 
                   DDIVWSYLNRQKKSLLGVDSSSVREQFRIVDRTSDKSGVSHYRLKQYVNGI 
                 
               
               
                   
               
               
                 
                   PVYGAEQTIHVGKSGEVTSYLGAVVNEDQQAEATQGTTPKISASEAVYTAY 
                 
               
               
                   
               
               
                 
                   KEAAARIEALPTSDDTISKDAEEPSSVSKDTYAEAANNEKTLSVDKDELSL 
                 
               
               
                   
               
               
                 
                   DQASVLKDSKIEAVEPEKSSIAKIANLQPEVDPKAELYYYPKGDDLLLVYV 
                 
               
               
                   
               
               
                   TEVNVLEPAPLRTRYIIDANDGSIVFQYDIINE ATGTGKGVLGDSKSFTTT 
               
               
                   
               
               
                 ASGSSYQLKDTTRGNGIVTYTASNRQSIPGTILTDADNVWNDPAGVDAHAY 
               
               
                   
               
               
                 AAKTYDYYKAKFGRNSIDGRGLQLRSTVHYGSRYNNAFWNGSQMTYGDGDG 
               
               
                   
               
               
                 STFIAFSGDPDVVGHELTHGVTEYTSNLEYYGESGALNEAFSDVIGNDIQR 
               
               
                   
               
               
                 KNWLVGDDIYTPNIAGDALRSMSNPTLYDQPDHYSNLYRGSSDNGGVHTNS 
               
               
                   
               
               
                 GIINKAYYLLAQGGNFHGVTVNGIGRDAAVQIYYSAFTNYLTSSSDFSNAR 
               
               
                   
               
               
                 AAVIQAAKDLYGANSAEATAAAKSFDAVGVN 
               
            
           
         
       
     
     The amino acid sequence of the predicted mature form of PpoPro1 is set forth as SEQ ID NO: 28: 
     
       
         
           
               
            
               
                 ATGTGKGVLGDSKSFTTTASGSSYQLKDTTRGNGIVTYTASNRQSIPGTIL 
               
               
                   
               
               
                 TDADNVWNDPAGVDAHAYAAKTYDYYKAKFGRNSIDGRGLQLRSTVHYGSR 
               
               
                   
               
               
                 YNNAFWNGSQMTYGDGDGSTFIAFSGDPDVVGHELTHGVTEYTSNLEYYGE 
               
               
                   
               
               
                 SGALNEAFSDVIGNDIQRKNWLVGDDIYTPNIAGDALRSMSNPTLYDQPDH 
               
               
                   
               
               
                 YSNLYRGSSDNGGVHTNSGIINKAYYLLAQGGNFHGVTVNGIGRDAAVQIY 
               
               
                   
               
               
                 YSAFTNYLTSSSDFSNARAAVIQAAKDLYGANSAEATAAAKSFDAVGVN 
               
            
           
         
       
     
     Example 6.2 
     Expression of  Paenibacillus polymyxa  SC2 Metalloprotease PpoPro1 
     The DNA sequence of the propeptide-mature form of PpoPro1 was synthesized and inserted into the  Bacillus subtilis  expression vector p2JM103BBI (Vogtentanz,  Protein Expr Purif,  55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX138(AprE-PpoPro1) ( FIG. 1 ). Ligation of this gene encoding the PpoPro1 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3′ end of the  B. subtilis  AprE signal sequence and the 5′ end of the predicted PpoPro1 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in  FIG. 6.1 , labeled pGX138(AprE-PpoPro1 contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in  B. subtilis , and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PpoPro1 (SEQ ID NO: 29). The translation product of the synthetic AprE-PpoPro1 gene is shown in SEQ ID NO: 30. 
     The pGX138(AprE-PpoPro1) plasmid was then transformed into  B. subtilis  cells (degU Hy  32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat #232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl 2 )). 
     The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl 2 ) and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to an 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above, PpoPro1 was eluted from the column with a linear salt gradient from 0 to 0.25 M NaCl in the loading buffer. The corresponding active fractions were collected and concentrated. The sample was loaded onto a 320 ml Superdex 75 gel filtration column pre-equilibrated with the loading buffer described above containing 0.15 M NaCl. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses. 
     The nucleotide sequence of the synthesized PpoPro1 gene in plasmid pGX138(AprE-PpoPro1) is depicted in SEQ ID NO: 29. The sequence encoding the three residue addition (AGK) is shown in bold: 
     
       
         
           
               
            
               
                 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAATC 
               
               
                   
               
               
                 TTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCT GCTGGAAAA GAATCA 
               
               
                   
               
               
                 TCAGTGTCAGGACCGGCTCAGCTTACACCGACATTTCACGCAGAACAATGG 
               
               
                   
               
               
                 AAGGCTCCGACGTCAGTTTCAGGAGACGACATCGTGTGGAGCTACCTGAAT 
               
               
                   
               
               
                 AGACAGAAGAAAAGCCTGCTGGGAGTGGATAGCAGCAGCGTCAGAGAGCAG 
               
               
                   
               
               
                 TTCAGAATCGTTGACAGAACGAGCGACAAAAGCGGAGTCAGCCATTATAGA 
               
               
                   
               
               
                 CTGAAGCAGTACGTGAATGGCATCCCGGTTTATGGCGCAGAGCAGACAATT 
               
               
                   
               
               
                 CATGTTGGCAAGAGCGGAGAAGTCACAAGCTATCTGGGCGCTGTGGTCAAT 
               
               
                   
               
               
                 GAAGATCAACAAGCCGAGGCTACACAGGGAACAACGCCGAAAATTAGCGCC 
               
               
                   
               
               
                 TCAGAGGCAGTCTACACGGCGTACAAAGAAGCGGCTGCAAGAATCGAAGCC 
               
               
                   
               
               
                 CTGCCGACATCAGACGATACAATTTCAAAAGATGCGGAGGAGCCGAGCTCA 
               
               
                   
               
               
                 GTTAGCAAGGATACATACGCGGAAGCCGCAAACAATGAGAAAACACTGAGC 
               
               
                   
               
               
                 GTGGACAAGGACGAGCTGTCACTTGATCAGGCTAGCGTCCTTAAAGACAGC 
               
               
                   
               
               
                 AAGATCGAGGCCGTTGAGCCTGAAAAGTCATCAATTGCGAAAATCGCCAAT 
               
               
                   
               
               
                 CTGCAACCTGAAGTCGACCCGAAGGCGGAACTGTACTACTACCCGAAAGGC 
               
               
                   
               
               
                 GATGACCTGCTTCTGGTGTACGTCACGGAAGTGAACGTCCTGGAACCGGCA 
               
               
                   
               
               
                 CCGCTGAGAACAAGATACATCATCGACGCGAACGACGGAAGCATCGTCTTC 
               
               
                   
               
               
                 CAGTATGACATTATCAACGAAGCAACGGGAACGGGCAAAGGCGTTCTTGGA 
               
               
                   
               
               
                 GACTCAAAGAGCTTCACGACAACGGCTTCAGGAAGCAGCTACCAGCTGAAA 
               
               
                   
               
               
                 GACACGACGAGAGGAAACGGAATCGTCACATATACGGCGTCAAACAGACAA 
               
               
                   
               
               
                 AGCATCCCTGGCACAATCCTGACGGATGCTGACAACGTTTGGAATGATCCG 
               
               
                   
               
               
                 GCTGGCGTGGATGCCCATGCTTATGCGGCAAAAACGTATGACTATTACAAG 
               
               
                   
               
               
                 GCGAAGTTCGGCAGAAATTCAATCGATGGCAGAGGACTGCAGCTTAGAAGC 
               
               
                   
               
               
                 ACGGTGCACTACGGATCAAGATATAACAATGCCTTCTGGAACGGCAGCCAG 
               
               
                   
               
               
                 ATGACATACGGAGACGGAGATGGAAGCACATTTATTGCATTCAGCGGCGAC 
               
               
                   
               
               
                 CCTGATGTGGTTGGCCATGAGCTGACGCATGGCGTTACAGAATATACGAGC 
               
               
                   
               
               
                 AATCTTGAATACTACGGCGAGTCAGGCGCTCTGAACGAGGCATTTAGCGAT 
               
               
                   
               
               
                 GTTATCGGCAATGACATCCAGAGAAAAAACTGGCTGGTGGGCGACGATATT 
               
               
                   
               
               
                 TACACGCCTAATATCGCTGGCGATGCCCTTAGATCAATGTCAAACCCGACG 
               
               
                   
               
               
                 CTGTATGATCAGCCTGACCACTACTCAAACCTGTATAGAGGCTCATCAGAT 
               
               
                   
               
               
                 AACGGAGGCGTCCATACGAATAGCGGCATCATTAACAAGGCATATTATCTT 
               
               
                   
               
               
                 CTGGCCCAGGGCGGCAATTTTCATGGAGTGACGGTTAATGGAATTGGAAGA 
               
               
                   
               
               
                 GACGCAGCCGTCCAAATCTACTACAGCGCTTTCACGAACTACCTTACATCA 
               
               
                   
               
               
                 AGCTCAGACTTTAGCAATGCCAGAGCTGCTGTTATCCAGGCAGCGAAGGAT 
               
               
                   
               
               
                 CTTTACGGCGCCAACTCAGCCGAAGCTACGGCCGCAGCTAAATCATTTGAT 
               
               
                   
               
               
                 GCAGTGGGCGTTAAT 
               
            
           
         
       
     
     The amino acid sequence of the PpoPro1 precursor protein expressed from plasmid pGX138(AprE-PpoPro1) is depicted in SEQ ID NO: 30. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text. 
     
       
         
           
               
            
               
                 
                   MRSKKLWISLLFALTLIFTMAFSNMSAQA 
                   AGK 
                   ESSVSGPAQLTPTFHAEQW 
                 
               
               
                   
               
               
                 
                   KAPTSVSGDDIVWSYLNRQKKSLLGVDSSSVREQFRIVDRTSDKSGVSHYR 
                 
               
               
                   
               
               
                 
                   LKQYVNGIPVYGAEQTIHVGKSGEVTSYLGAVVNEDQQAEATQGTTPKISA 
                 
               
               
                   
               
               
                 
                   SEAVYTAYKEAAARIEALPTSDDTISKDAEEPSSVSKDTYAEAANNEKTLS 
                 
               
               
                   
               
               
                 
                   VDKDELSLDQASVLKDSKIEAVEPEKSSIAKIANLQPEVDPKAELYYYPKG 
                 
               
               
                   
               
               
                   DDLLLVYVTEVNVLEPAPLRTRYIIDANDGSIVFQYDIINE ATGTGKGVLG 
               
               
                   
               
               
                 DSKSFTTTASGSSYQLKDTTRGNGIVTYTASNRQSIPGTILTDADNVWNDP 
               
               
                   
               
               
                 AGVDAHAYAAKTYDYYKAKFGRNSIDGRGLQLRSTVHYGSRYNNAFWNGSQ 
               
               
                   
               
               
                 MTYGDGDGSTFIAFSGDPDVVGHELTHGVTEYTSNLEYYGESGALNEAFSD 
               
               
                   
               
               
                 VIGNDIQRKNWLVGDDIYTPNIAGDALRSMSNPTLYDQPDHYSNLYRGSSD 
               
               
                   
               
               
                 NGGVHTNSGIINKAYYLLAQGGNFHGVTVNGIGRDAAVQIYYSAFTNYLTS 
               
               
                   
               
               
                 SSDFSNARAAVIQAAKDLYGANSAEATAAAKSFDAVGVN 
               
            
           
         
       
     
     Example 6.3 
     Proteolytic Activity of Metalloprotease PpoPro1 
     The proteolytic activity of purified PpoPro1 was measured in 50 mM Tris (pH 7), using azo-casein (Cat #74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μL of the diluted enzyme (or Milli-Q H 2 O alone as the blank control) was added to the non-binding 96-well microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μL of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μL of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μL supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A 440 ) using a SpectraMax 190. Net A 440  was calculated by subtracting the A 440  of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of duplicate assays, and the value varies no more than 5%. The proteolytic activity is shown as Net A 44 o. The proteolytic assay with azo-casein as the substrate ( FIG. 6.2 ) indicates PpoPro1 is an active protease. 
     Example 4 
     pH Profile of Metalloprotease PpoPro1 
     With azo-casein as the substrate, the pH profile of PpoPro1 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μL of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μL diluted enzyme (250 ppm in Milli-Q H 2 O) in a 96-MTP placed on ice, followed by the addition of 48 μL of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 6.3. Enzyme activity at each pH was reported as relative activity where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in  FIG. 6.3 , the optimal pH of PpoPro1 is about 7, with greater than 70% of maximal activity retained between 5.5 and 8.5. 
     Example 6.5 
     Temperature Profile of Metalloprotease PpoPro1 
     The temperature profile of PpoPro1 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assay. The enzyme sample and azo-casein substrate were prepared as in Example 6.3. Prior to the reaction, 50 μL of 1.5% azo-casein and 45 μl Milli-Q H 2 O were mixed in a 2000_, PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20-90° C.) for 5 min. After the incubation, 5 μL of diluted PpoPro1 (100 ppm) or H 2 O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μL of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 6.3. The activity was reported as relative activity where the activity at the optimal temperature was set to be 100%. The tested temperatures were 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in  FIG. 6.4  suggests that PpoPro1 showed an optimal temperature at 50° C., and retained greater than 70% of its maximum activity between 40 and 55° C. 
     Example 6.6 
     Cleaning Performance of Metalloprotease PpoPro1 
     The cleaning performance of PpoPro1 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 or 8 using a model automatic dishwashing (ADW) detergent (AT detergent). Prior to the reaction, purified PpoPro1 was diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl 2 , 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent (composition shown in Table 6.1) with 100 ppm water hardness (Ca 2+ :Mg 2+ =3:1), in the presence of a bleach component ((Peracid N,N-phthaloylaminoperoxycaproic acid-PAP). To initiate the reaction, 180 μL of AT detergent buffered at pH 6 or 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μL of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μL of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the “Initial performance”) using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μL water. Following the addition of 180 μL of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliter of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as “Wash-off”). The sum of two absorbance measurements (“Initial performance” plus “Wash-off”) gives the “Total performance”, which measures the protease activity on the model stain; and Net A 405  was subsequently calculated by subtracting the A 405  of the “Total performance” of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 for PpoPro1 in AT dish detergent, in the presence of bleach, is shown in  FIGS. 6.5A and 6.5B . 
     
       
         
           
               
             
               
                 TABLE 6.1 
               
             
            
               
                   
               
               
                 Composition of AT dish detergent formula with bleach 
               
            
           
           
               
               
               
            
               
                   
                   
                 Concentration  
               
               
                   
                 Ingredient 
                 (mg/ml) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 MGDA (methylglycinediacetic acid) 
                 0.143 
               
               
                   
                 Sodium citrate 
                 1.86 
               
               
                   
                 Citric acid* 
                 varies 
               
               
                   
                 PAP (peracid N,N-phthaloylaminoperoxycaproic  
                 0.057 
               
               
                   
                 acid) 
                   
               
               
                   
                 Plurafac ® LF 18B (a non-ionic surfactant) 
                 0.029 
               
               
                   
                 Bismuthcitrate 
                 0.006 
               
               
                   
                 Bayhibit ® S (Phosphonobutantricarboxylic 
                 0.006 
               
               
                   
                 acid sodium salt) 
                   
               
               
                   
                 Acusol ™ 587 (a calcium polyphosphate 
                 0.029 
               
               
                   
                 inhibitor) 
                   
               
               
                   
                 PEG 6000 
                 0.043 
               
               
                   
                 PEG 1500 
                 0.1 
               
               
                   
                   
               
               
                   
                 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid. 
               
            
           
         
       
     
     Example 6.7 
     Comparison of PpoPro1 to Other Metalloproteases 
     Identification of Homologous Proteases 
     Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The predicted mature protein amino acid sequence for PpoPro1 (SEQ ID NO: 28) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 6.2A and 6.2B provide a list of sequences with the percent identity to PpoPro1. The length in Table 6.2 refers to the entire sequence length of the homologous proteases. 
     
       
         
           
               
             
               
                 TABLE 6.2A 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PpoPro1 protein 
               
               
                 identified from the NCBI non-redundant protein database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Accession # 
                 PpoPro1 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 P00800 
                 56 
                 
                   Bacillus thermoproteolyticus 
                 
                 548 
               
               
                 ZP_08640523.1 
                 57 
                   Brevibacillus laterosporus  LMG 
                 564 
               
               
                   
                   
                 15441 
                   
               
               
                 AAA22623.1 
                 57 
                 
                   Bacillus caldolyticus 
                 
                 544 
               
               
                 ZP_08093424.1 
                 59 
                   Planococcus donghaensis  MPA1U2 
                 553 
               
               
                 ZP_10738945.1 
                 60 
                   Brevibacillus  sp. CF112 
                 528 
               
               
                 CAA43589.1 
                 62 
                 
                   Brevibacillus brevis 
                 
                 527 
               
               
                 ZP_02326503.1 
                 62 
                   Paenibacillus larvae  subsp. 
                 520 
               
               
                   
                   
                   larvae  BRL-230010 
                   
               
               
                 YP_005495105.1 
                 63 
                   Bacillus   megaterium  WSH-002 
                 562 
               
               
                 YP_001373863.1 
                 64 
                   Bacillus   cytotoxicus  NVH 391-98 
                 565 
               
               
                 ZP_04310163.1 
                 64 
                   Bacillus   cereus  BGSC 6E1 
                 581 
               
               
                 BAA06144.1 
                 64 
                   Lactobacillus  sp.] 
                 566 
               
               
                 ZP_08511445.1 
                 65 
                   Paenibacillus  sp. HGF7 
                 525 
               
               
                 ZP_04216147.1 
                 65 
                   Bacillus   cereus  Rock3-44 
                 566 
               
               
                 ZP_09071078.1 
                 68 
                   Paenibacillus   larvae  subsp.  larvae  B- 
                   
               
               
                   
                   
                 3650 
                   
               
               
                 ZP_09077634.1 
                 69 
                   Paenibacillus   elgii  B69 
                 524 
               
               
                 YP_005073224.1 
                 79 
                   Paenibacillus   terrae  HPL-003 
                 595 
               
               
                 ZP_10241029.1 
                 80 
                   Paenibacillus   peoriae  KCTC 3763 
                 599 
               
               
                 YP_005073223.1 
                 93 
                   Paenibacillus terrae  HPL-003 
                 591 
               
               
                 ZP_10241030.1 
                 95 
                   Paenibacillus  peoriae KCTC 3763 
                 593 
               
               
                 ZP_09775364.1 
                 95 
                   Paenibacillus  sp. Aloe-11 
                 593 
               
               
                 YP_003872179.1 
                 97 
                   Paenibacillus   polymyxa  E681 
                 592 
               
               
                 YP_003948511.1 
                 100 
                   Paenibacillus polymyxa  SC2 
                 592 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6.2B 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PpoPro1 protein identified 
               
               
                 from the Genome Quest Patent database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Patent # 
                 PpoPro1 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 US20120107907-0187 
                 97.34 
                 
                   Bacillus polymyxa 
                 
                 302 
               
               
                 US5962264-0004 
                 65.48 
                 empty 
                 566 
               
               
                 WO2012110563-0005 
                 65.16 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 JP1994070791-0002 
                 64.52 
                 empty 
                 317 
               
               
                 WO2012110562-0005 
                 64.19 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 WO2012110563-0004 
                 63.34 
                 
                   Bacillus megaterium 
                 
                 320 
               
               
                 JP2002272453-0002 
                 61.98 
                 
                   Bacillus megaterium 
                 
                 562 
               
               
                 WO2004011619-0047 
                 61.49 
                 empty 
                 532 
               
               
                 EP2390321-0186 
                 62.58 
                 
                   Bacillus brevis 
                 
                 304 
               
               
                 US6518054-0002 
                 59.22 
                   Bacillus  sp. 
                 316 
               
               
                 US6518054-0001 
                 58.52 
                   Bacillus  sp. 
                 319 
               
               
                 US20120107907-0176 
                 58.52 
                 
                   Bacillus stearothermophilis 
                 
                 548 
               
               
                 JP2005229807-0019 
                 93.05 
                 
                   Paenibacillus polymyxa 
                 
                 566 
               
               
                 WO2012110562-0003 
                 58.2 
                 
                   Geobacillus 
                 
                 319 
               
               
                   
                   
                 
                   stearothermophilus 
                 
                   
               
               
                 WO2004011619-0044 
                 59.27 
                 empty 
                 507 
               
               
                 EP2390321-0185 
                 66.13 
                 
                   Bacillus cereus 
                 
                 317 
               
               
                 JP1995184649-0001 
                 65.71 
                   Lactobacillus  sp. 
                 566 
               
               
                 EP2178896-0184 
                 65.38 
                 
                   Bacillus anthracis 
                 
                 566 
               
               
                   
               
            
           
         
       
     
     Alignment of Homologous Protease Sequences 
     The amino acid sequence of predicted mature PpoPro1 (SEQ ID NO: 28) was aligned with thermolysin (P00800,  Bacillus thermoproteolyticus ) and protease from  Paenibacillus polymyxa  SC2 (YP 003948511.1) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters.  FIG. 6.6  shows the alignment of PpoPro1 with these protease sequences. 
     Phylogenetic Tree 
     A phylogenetic tree for precursor PpoPro1 (SEQ ID NO: 27) was built using sequences of representative homologs from Tables 6.2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. MolBiol.Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in  FIG. 6.7 . 
     Example 7.1 
     Cloning of  Paenibacillus hunanensis  Metalloprotease PhuPro1 
     A strain (DSM22170) of  Paenibacillus hunanensis  was selected as a potential source of enzymes which may be useful in various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the  Paenibacillus hunanensis  strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina&#39;s next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in  Paenibacillus hunanensis  encodes a metalloprotease and the sequence of this gene, called PhuPro1, is provided in SEQ ID NO: 31. This gene has an alternative start codon (TTG). The corresponding protein encoded by the PhuPro1 gene is shown in SEQ ID NO: 32. At the N-terminus, the protein has a signal peptide with a length of 23 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PhuPro1 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the  Paenibacillus polymyxa  Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PhuPro1 protein is shown in SEQ ID NO: 33. 
     The nucleotide sequence of the PhuPro1 gene isolated from  Paenibacillus hunanensis  is set forth as SEQ ID NO: 31. The sequence encoding the predicted native signal peptide is shown in italics: 
     
       
         
           
               
            
               
                 
                   TTGAAAAAAACAGTTGGTCTTTTACTTGCAGGTAGCTTGCTCGTTGGTGCT 
                 
               
               
                   
               
               
                   ACAACGTCCGCTTTCGCA GCAGAAGCAAATGATCTGGCACCACTCGGTGAT 
               
               
                   
               
               
                 TACACGCCAAAATTGATTACGCAAGCAACAGGCATCACTGGCGCTAGTGGC 
               
               
                   
               
               
                 GATGCTAAAGTATGGAAGTTCCTGGAGAAGCAAAAACGTACCATCGTAACC 
               
               
                   
               
               
                 GATGATGCAGCTTCTGCTGATGTGAAGGAATTGTTTGAGATCACAAAACGT 
               
               
                   
               
               
                 CAATCCGATTCTCAAACCGGTACAGAGCACTATCGCCTGAACCAAACCTTT 
               
               
                   
               
               
                 AAAGGCATCCCAGTCTATGGCGCAGAGCAAACACTGCACTTTGACAAATCC 
               
               
                   
               
               
                 GGCAATGTATCTCTGTACATGGGTCAGGTTGTTGAGGATGTGTCCGCTAAA 
               
               
                   
               
               
                 CTGGAAGCTTCCGATTCCAAAAAAGGCGTAACTGAGGATGTATACGCTTCG 
               
               
                   
               
               
                 GATACGAAAAATGATCTGGTAACACCAGAAATCAGCGCTTCTCAAGCCATC 
               
               
                   
               
               
                 TCGATTGCTGAAAAGGATGCAGCTTCCAAAATCGGCTCCCTCGGCGAAGCA 
               
               
                   
               
               
                 CAAAAAACGCCAGAAGCGAAGCTGTATATCTACGCTCCTGAGGATCAAGCA 
               
               
                   
               
               
                 GCACGTCTGGCTTATGTGACAGAAGTAAACGTACTGGAGCCATCTCCGCTG 
               
               
                   
               
               
                 CGTACTCGCTATTTTGTAGATGCAAAAACAGGTTCGATCCTGTTCCAATAT 
               
               
                   
               
               
                 GATCTGATTGAGCATGCAACAGGTACAGGTAAAGGGGTACTGGGTGATACC 
               
               
                   
               
               
                 AAGTCCTTCACTGTAGGTACTTCCGGTTCTTCCTATGTGATGACTGATAGC 
               
               
                   
               
               
                 ACGCGTGGAAAAGGTATCCAAACCTACACGGCGTCTAACCGCACATCACTG 
               
               
                   
               
               
                 CCAGGTAGCACTGTAACGAGCAGCAGCAGCACATTTAACGATCCAGCATCT 
               
               
                   
               
               
                 GTCGATGCCCATGCGTATGCACAAAAAGTATATGATTTCTACAAATCCAAC 
               
               
                   
               
               
                 TTTAACCGCAACAGCATCGACGGTAATGGTCTGGCTATCCGCTCCACTACG 
               
               
                   
               
               
                 CACTATTCCACACGTTATAACAATGCGTTCTGGAATGGTTCCCAAATGGTA 
               
               
                   
               
               
                 TACGGTGATGGCGATGGTTCGCAATTCATCGCATTCTCCGGCGACCTTGAC 
               
               
                   
               
               
                 GTAGTAGGTCACGAGCTGACACACGGTGTAACCGAGTACACAGCGAACCTG 
               
               
                   
               
               
                 GAATACTATGGTCAATCCGGTGCACTGAACGAATCCATTTCGGATATCTTT 
               
               
                   
               
               
                 GGTAACACAATCGAAGGTAAAAACTGGATGGTAGGCGATGCGATCTACACA 
               
               
                   
               
               
                 CCAGGCGTATCCGGCGATGCTCTTCGCTACATGGATGATCCAACAAAAGGT 
               
               
                   
               
               
                 GGACAACCAGCGCGTATGGCAGATTACAACAACACAAGCGCTGATAATGGC 
               
               
                   
               
               
                 GGTGTACACACAAACAGTGGTATCCCGAATAAAGCATACTACTTGCTGGCA 
               
               
                   
               
               
                 CAGGGTGGCACATTTGGCGGTGTAAATGTAACAGGTATCGGTCGCTCGCAA 
               
               
                   
               
               
                 GCGATCCAGATCGTTTACCGTGCACTAACATACTACCTGACATCCACATCT 
               
               
                   
               
               
                 AACTTCTCGAACTACCGTTCTGCAATGGTGCAAGCATCTACAGACCTGTAC 
               
               
                   
               
               
                 GGTGCAAACTCTACACAAACAACAGCGGTGAAAAACTCGCTGAGCGCAGTA 
               
               
                   
               
               
                 GGCATTAAC 
               
            
           
         
       
     
     The amino acid sequence of the PhuPro1 precursor protein is set forth as SEQ ID NO: 32. The predicted signal sequence is shown in italics, and the predicted pro-peptide is shown in underlined text: 
     
       
         
           
               
            
               
                 
                   MKKTVGLLLAGSLLVGATTSAFA 
                   AEANDLAPLGDYTPKLITQATGITGASG 
                 
               
               
                   
               
               
                 
                   DAKVWKFLEKQKRTIVTDDAASADVKELFEITKRQSDSQTGTEHYRLNQTF 
                 
               
               
                   
               
               
                 
                   KGIPVYGAEQTLHFDKSGNVSLYMGQVVEDVSAKLEASDSKKGVTEDVYAS 
                 
               
               
                   
               
               
                 
                   DTKNDLVTPEISASQAISIAEKDAASKIGSLGEAQKTPEAKLYIYAPEDQA 
                 
               
               
                   
               
               
                   ARLAYVTEVNVLEPSPLRTRYFVDAKTGSILFQYDLIEH ATGTGKGVLGDT 
               
               
                   
               
               
                 KSFTVGTSGSSYVMTDSTRGKGIQTYTASNRTSLPGSTVTSSSSTFNDPAS 
               
               
                   
               
               
                 VDAHAYAQKVYDFYKSNFNRNSIDGNGLAIRSTTHYSTRYNNAFWNGSQMV 
               
               
                   
               
               
                 YGDGDGSQFIAFSGDLDVVGHELTHGVTEYTANLEYYGQSGALNESISDIE 
               
               
                   
               
               
                 GNTIEGKNAVMVGDAIYTPGVSGDALRYMDDPTKGGQPARMADYNNTSADN 
               
               
                   
               
               
                 GGVHTNSGIPNKAYYLLAQGGTFGGVNVTGIGRSQAIQIVYRALTYYLTST 
               
               
                   
               
               
                 SNFSNYRSAMVQASTDLYGANSTQTTAVKNSLSAVGIN 
               
            
           
         
       
     
     The amino acid sequence of the predicted mature form of PhuPro1 is set forth as SEQ ID NO: 33: 
     
       
         
           
               
            
               
                 ATGTGKGVLGDTKSFTVGTSGSSYVMTDSTRGKGIQTYTASNRTSLPGSTV 
               
               
                   
               
               
                 TSSSSTFNDPASVDAHAYAQKVYDFYKSNFNRNSIDGNGLAIRSTTHYSTR 
               
               
                   
               
               
                 YNNAFWNGSQMVYGDGDGSQFIAFSGDLDVVGHELTHGVTEYTANLEYYGQ 
               
               
                   
               
               
                 SGALNESISDIFGNTIEGKNWMVGDAIYTPGVSGDALRYMDDPTKGGQPAR 
               
               
                   
               
               
                 MADYNNTSADNGGVHTNSGIPNKAYYLLAQGGTFGGVNVTGIGRSQAIQIV 
               
               
                   
               
               
                 YRALTYYLTSTSNFSNYRSAMVQASTDLYGANSTQTTAVKNSLSAVGIN 
               
            
           
         
       
     
     Example 7.2 
     Expression of  Paenibacillus hunanensis  Metalloprotease PhuPro1 
     The DNA sequence of the propeptide-mature form of PhuPro1 was synthesized and inserted into the  Bacillus subtilis  expression vector p2JM103BBI (Vogtentanz,  Protein Expr Purif,  55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX149(AprE-PhuPro1) ( FIG. 7.1 ). Ligation of this gene encoding the PhuPro1 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3′ end of the  B. subtilis  AprE signal sequence and the 5′ end of the predicted PhuPro1 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in  FIG. 1 , labeled pGX149(AprE-PhuPro1) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in  B. subtilis , and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PhuPro1 (SEQ ID NO: 34). The translation product of the synthetic AprE-PhuPro1 gene is shown in SEQ ID NO: 35. 
     The pGX149(AprE-PhuPro1) plasmid was then transformed into  B. subtilis  cells (degU Hy  32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat #232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl 2 ). 
     The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl 2  and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to a 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above; and the active flow-through fractions were collected and concentrated via 10K Amicon Ultra for further analyses. 
     The nucleotide sequence of the synthesized PhuPro1 gene in plasmid pGX149(AprE-PhuPro1) is depicted in SEQ ID NO: 34. The sequence encoding the three residue addition (AGK) is shown in bold: 
     
       
         
           
               
            
               
                 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAATC 
               
               
                   
               
               
                 TTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCT GCTGGAAAA GCAGAA 
               
               
                   
               
               
                 GCTAATGATCTTGCCCCGCTTGGCGATTATACACCGAAGCTTATTACACAG 
               
               
                   
               
               
                 GCAACGGGAATTACAGGCGCATCAGGCGATGCGAAGGTGTGGAAGTTCCTG 
               
               
                   
               
               
                 GAGAAGCAGAAGAGAACGATTGTCACGGACGACGCCGCAAGCGCGGATGTC 
               
               
                   
               
               
                 AAGGAGCTGTTCGAGATCACGAAGAGACAGAGCGATAGCCAGACGGGAACG 
               
               
                   
               
               
                 GAGCATTACAGACTGAACCAGACGTTCAAGGGCATTCCGGTCTACGGAGCT 
               
               
                   
               
               
                 GAACAAACGCTGCATTTTGATAAAAGCGGCAACGTCTCACTGTACATGGGC 
               
               
                   
               
               
                 CAAGTCGTTGAGGACGTTAGCGCCAAACTTGAGGCTAGCGACAGCAAGAAA 
               
               
                   
               
               
                 GGCGTCACAGAAGATGTCTACGCGTCAGACACGAAAAACGACCTGGTTACA 
               
               
                   
               
               
                 CCGGAAATCTCAGCTTCACAGGCCATCTCAATTGCAGAGAAAGACGCAGCG 
               
               
                   
               
               
                 TCAAAAATCGGCTCACTGGGCGAGGCTCAGAAAACGCCGGAGGCGAAACTT 
               
               
                   
               
               
                 TACATCTACGCCCCTGAGGACCAGGCTGCGAGACTGGCTTACGTGACAGAA 
               
               
                   
               
               
                 GTTAATGTGCTGGAGCCGTCACCGCTTAGAACGAGATATTTCGTGGACGCA 
               
               
                   
               
               
                 AAGACGGGCAGCATTCTGTTTCAGTACGATCTTATCGAACACGCGACAGGC 
               
               
                   
               
               
                 ACAGGAAAGGGAGTTCTGGGAGACACAAAAAGCTTCACGGTTGGCACGTCA 
               
               
                   
               
               
                 GGCAGCAGCTACGTGATGACAGACAGCACGAGAGGCAAGGGCATTCAAACG 
               
               
                   
               
               
                 TATACAGCGAGCAACAGAACAAGCCTGCCGGGAAGCACAGTCACGAGCTCA 
               
               
                   
               
               
                 TCATCAACGTTTAATGACCCGGCCTCAGTGGATGCTCACGCATACGCGCAG 
               
               
                   
               
               
                 AAAGTGTACGACTTCTACAAAAGCAACTTCAATAGAAACAGCATCGACGGA 
               
               
                   
               
               
                 AACGGCCTTGCGATCAGAAGCACGACGCACTACAGCACAAGATACAACAAC 
               
               
                   
               
               
                 GCCTTCTGGAACGGCAGCCAAATGGTTTACGGCGATGGCGACGGATCACAG 
               
               
                   
               
               
                 TTTATCGCATTTAGCGGAGACCTGGACGTCGTTGGCCATGAGCTGACACAT 
               
               
                   
               
               
                 GGCGTTACGGAGTACACAGCAAACCTGGAATACTATGGCCAGTCAGGCGCC 
               
               
                   
               
               
                 CTTAACGAGAGCATCAGCGACATTTTTGGCAATACGATCGAAGGAAAGAAC 
               
               
                   
               
               
                 TGGATGGTCGGCGACGCAATCTACACACCGGGCGTTTCAGGCGATGCACTG 
               
               
                   
               
               
                 AGATATATGGACGACCCGACAAAGGGCGGACAGCCGGCCAGAATGGCGGAT 
               
               
                   
               
               
                 TACAATAATACGTCAGCAGATAACGGCGGCGTGCATACAAATAGCGGCATC 
               
               
                   
               
               
                 CCTAACAAAGCATATTACCTGCTTGCGCAAGGAGGAACATTTGGCGGCGTG 
               
               
                   
               
               
                 AATGTTACGGGCATTGGCAGATCACAAGCGATTCAGATCGTTTACAGAGCG 
               
               
                   
               
               
                 CTGACGTACTACCTTACGAGCACGAGCAATTTTAGCAACTACAGAAGCGCA 
               
               
                   
               
               
                 ATGGTGCAGGCAAGCACGGATCTGTATGGCGCAAATTCAACACAAACGACG 
               
               
                   
               
               
                 GCGGTCAAGAATAGCCTTTCAGCAGTGGGCATTAACTAA 
               
            
           
         
       
     
     The amino acid sequence of the PhuPro1 precursor protein expressed from plasmid pGX149(AprE-PhuPro1) is depicted in SEQ ID NO: 35. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text. 
     
       
         
           
               
            
               
                 
                   MRSKKLWISLLFALTLIFTMAFSNMSAQA 
                   AGK 
                   AEANDLAPLGDYTPKLITQ 
                 
               
               
                   
               
               
                 
                   ATGITGASGDAKVWKFLEKQKRTIVTDDAASADVKELFEITKRQSDSQTGT 
                 
               
               
                   
               
               
                 
                   EHYRLNQTFKGIPVYGAEQTLHFDKSGNVSLYMGQVVEDVSAKLEASDSKK 
                 
               
               
                   
               
               
                 
                   GVTEDVYASDTKNDLVTPEISASQAISIAEKDAASKIGSLGEAQKTPEAKL 
                 
               
               
                   
               
               
                   YIYAPEDQAARLAYVTEVNVLEPSPLRTRYFVDAKTGSILFQYDLIEH ATG 
               
               
                   
               
               
                 TGKGVLGDTKSFTVGTSGSSYVMTDSTRGKGIQTYTASNRTSLPGSTVTSS 
               
               
                   
               
               
                 SSTFNDPASVDAHAYAQKVYDFYKSNFNRNSIDGNGLAIRSTTHYSTRYNN 
               
               
                   
               
               
                 AFWNGSQMVYGDGDGSQFIAFSGDLDVVGHELTHGVTEYTANLEYYGQSGA 
               
               
                   
               
               
                 LNESISDIFGNTIEGKNWMVGDAIYTPGVSGDALRYMDDPTKGGQPARMAD 
               
               
                   
               
               
                 YNNTSADNGGVHTNSGIPNKAYYLLAQGGTFGGVNVTGIGRSQAIQIVYRA 
               
               
                   
               
               
                 LTYYLTSTSNFSNYRSAMVQASTDLYGANSTQTTAVKNSLSAVGIN 
               
            
           
         
       
     
     Example 7.3 
     Proteolytic Activity of Metalloprotease PhuPro1 
     The proteolytic activity of purified metalloprotease PhuPro1 was measured in 50 mM Tris (pH 7), using azo-casein (Cat #74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H 2 O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A 440 ) using a SpectraMax 190. Net A 440  was calculated by subtracting the A 440  of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of triplicate assays. The proteolytic activity is shown as Net A 440 . The proteolytic assay with azo-casein as the substrate (shown in  FIG. 7.2 ) indicates that PhuPro1 is an active protease. 
     Example 7.4 
     pH Profile of Metalloprotease PhuPro1 
     With azo-casein as the substrate, the pH profile of metalloprotease PhuPro1 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl Milli-Q H 2 O diluted enzyme (125 ppm) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in  FIG. 7.3 , the optimal pH of PhuPro1 is about 6, with greater than 70% of maximal activity retained between 5 and 8. 
     Example 7.5 
     Temperature Profile of Metalloprotease PhuPro1 
     The temperature profile of metalloprotease PhuPro1 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assays. The enzyme sample and azo-casein substrate were prepared as in Example 7.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H 2 O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20-90° C.) for 5 min. After the incubation, 5 μl of diluted enzyme (50 ppm) or H 2 O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 7.3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in  FIG. 7.4  suggests that PhuPro1 showed an optimal temperature at 60° C., and retained greater than 70% of its maximum activity between 45 and 65° C. 
     Example 7.6 
     Cleaning Performance of Metalloprotease PhuPro1 
     The cleaning performance of PhuPro1 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified protease samples were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl 2 , 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca 2+ :Mg 2+ =3:1) (detergent composition shown in Table 7.1). To initiate the reaction, 180 μl of the AT detergent buffered at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the “Initial performance”) using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as the “Wash-off”). The sum of two absorbance measurements (“Initial performance” plus “Wash-off”) gives the “Total performance”, which measures the protease activity on the model stain; and Net A 405  was subsequently calculated by subtracting the A 405  of the “Total performance” of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 in AT detergent for PhuPro1 is shown in  FIGS. 7.5A and 7.5B . 
     
       
         
           
               
             
               
                 TABLE 7.1 
               
             
            
               
                   
               
               
                 Composition of AT detergent 
               
            
           
           
               
               
               
            
               
                   
                   
                 Concentration  
               
               
                   
                 Ingredient 
                 (mg/ml) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 MGDA (methylglycinediacetic acid) 
                 0.143 
               
               
                   
                 Sodium citrate 
                 1.86 
               
               
                   
                 Citric acid* 
                 varies 
               
               
                   
                 Plurafac ® LF 18B (a non-ionic surfactant) 
                 0.029 
               
               
                   
                 Bismuthcitrate 
                 0.006 
               
               
                   
                 Bayhibit ® S (Phosphonobutantricarboxylic  
                 0.006 
               
               
                   
                 acid sodium salt) 
                   
               
               
                   
                 Acusol ™ 587 (a calcium polyphosphate  
                 0.029 
               
               
                   
                 inhibitor) 
                   
               
               
                   
                 PEG 6000 
                 0.043 
               
               
                   
                 PEG 1500 
                 0.1 
               
               
                   
                   
               
               
                   
                 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid. 
               
            
           
         
       
     
     Example 7.7 
     Comparison of PhuPro1 to Other Proteases 
     A. Identification of Homologous Proteases 
     Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The predicted mature protein amino acid sequence for PhuPro1 (SEQ ID NO: 33) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 7.2A and 7.2B provide a list of sequences with the percent identity to PhuPro1. The length in Table 7.2 refers to the entire sequence length of the homologous proteases. 
     
       
         
           
               
             
               
                 TABLE 7.2A 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PhuPro1 protein identified 
               
               
                 from the NCBI non-redundant protein database 
               
            
           
           
               
               
               
               
            
               
                 Accession # 
                 PID to PhuPro1  
                 Organism 
                 Length 
               
               
                   
               
               
                 P00800 
                 55 
                 
                   Bacillus thermoproteolyticus 
                 
                 548 
               
               
                 AAB02774.1 
                 55 
                 
                   Geobacillus stearothermophilus 
                 
                 552 
               
               
                 EJS73098.1 
                 56 
                   Bacillus cereus  BAG2X1-3 
                 566 
               
               
                 BAD60997.1 
                 56 
                 
                   Bacillus megaterium 
                 
                 562 
               
               
                 ZP_04216147.1 
                 57 
                   Bacillus cereus  Rock3-44 
                 566 
               
               
                 YP_893436.1 
                 56 
                   Bacillus thuringiensis  str.  Al Hakam   
                 566 
               
               
                 ZP_08640523.1 
                 58 
                 
                   Brevibacillus laterosporus 
                 
                 564 
               
               
                 ZP_09069194.1 
                 59 
                   Paenibacillus larvae  subsp.  larvae  B-3650 
                 502 
               
               
                 YP_002770810.1 
                 60 
                 
                   Brevibacillus brevis 
                 
                 528 
               
               
                 ZP_08511445.1 
                 61 
                   Paenibacillus  sp. HGF7 
                 525 
               
               
                 P43263 
                 61 
                 
                   Brevibacillus brevis 
                 
                 527 
               
               
                 ZP_09775365.1 
                 62 
                   Paenibacillus  sp. Aloe-11 
                 580 
               
               
                 ZP_09077634.1 
                 66 
                   Paenibacillus elgii  B69 
                 524 
               
               
                 P29148 
                 68 
                 NPRE_PAEPO 
                 590 
               
               
                 ZP_09775364.1 
                 69 
                   Paenibacillus  sp. Aloe-11 
                 593 
               
               
                 ZP_10241030.1 
                 69 
                   Paenibacillus peoriae  KCTC 3763 
                 593 
               
               
                 YP_005073223.1 
                 69 
                   Paenibacillus terrae  HPL-003 
                 591 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7.2B 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PhuPro1 protein 
               
               
                 identified from the Genome Quest Patent database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Patent ID # 
                 PhuPro1 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 WO2012110562-0003 
                 56.23 
                 
                   Geobacillus stearothermophilus 
                 
                 319 
               
               
                 US6518054-0001 
                 56.55 
                   Bacillus  sp. 
                 319 
               
               
                 JP2002272453-0002 
                 56.69 
                 
                   Bacillus megaterium 
                 
                 562 
               
               
                 US20090123467-0184 
                 56.73 
                 
                   Bacillus anthracis 
                 
                 566 
               
               
                 US6103512-0003 
                 56.87 
                   
                 319 
               
               
                 EP0867512-0002 
                 56.96 
                   
                 316 
               
               
                 WO2012110562-0005 
                 57.1 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 WO2012110563-0005 
                 58.06 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 US20120107907-0187 
                 68.44 
                 
                   Bacillus polymyxa 
                 
                 302 
               
               
                   
               
            
           
         
       
     
     B. Alignment of Homologous Protease Sequences 
     The amino acid sequence of predicted mature PhuPro1 (SEQ ID NO: 33) protein was aligned with Proteinase T (P00800,  Bacillus thermoproteolyticus ), and protease from  Paenibacillus terrae  HPL-003 (YP 005073223.1) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters.  FIG. 7.6  shows the alignment of PhuPro1 with these protease sequences. 
     C. Phylogenetic Tree 
     A phylogenetic tree for full length sequence of PhuPro1 (SEQ ID NO: 2) was built using sequences of representative homologs from Table 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. MolBiol.Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in  FIG. 7.7 . 
     Example 7.8 
     Terg-o-Tometer Performance Evaluation of PhuPro1 
     The wash performance of PhuPro1 was tested in a laundry detergent application using a Terg-o-Tometer (Instrument Marketing Services, Inc, Fairfield, N.J.). The performance evaluation was conducted at 32° C. and 16° C. The soil load consisted of two of each of the following stain swatches: EMPA116 Blood, Milk, Ink on cotton (Test materials AG, St. Gallen, Switzerland), EMPA117 Blood, Milk, Ink on polycotton (Test materials AG, St. Gallen, Switzerland), EMPA112 Cocoa on cotton (Test materials AG, St. Gallen, Switzerland), and CFT C-10 Pigment, Oil, and Milk content on cotton (Center for Testmaterials BV, Vlaardingen, Netherlands), plus extra white interlock knit fabric to bring the total fabric load to 40 g per beaker of the Terg-o-Tometer, which was filled with 1 L of deionized water. The water hardness was adjusted to 6 grains per gallon, and the pH in the beaker was buffered with 5 mM HEPES, pH 8.2. Heat inactivated Tide Regular HDL (Proctor &amp; Gamble), a commercial liquid detergent purchased in a local US supermarket, was used at 0.8 g/L. The detergent was inactivated before use by treatment at 92° C. in a water bath for 2-3 hours followed by cooling to room temperature. Heat inactivation of commercial detergents serves to destroy the activity of enzymatic components while retaining the properties of the non-enzymatic components. Enzyme activity in the heat inactivated detergent was measured using the Suc-AAPF-pNA assay for measuring protease activity. The Purafect® Prime HA, (Genencor Int&#39;l) and PhuPro1 proteases were each added to final concentrations of 1 ppm. A control sample with no enzyme was included. The wash time was 12 minutes. After the wash treatment, all swatches were rinsed for 3 minutes and machine-dried at low heat. 
     Four of each type of swatch were measured before and after treatment by optical reflectance using a Tristimulus Minolta Meter CR-400. The difference in the L, a, b values was converted to total color difference (dE), as defined by the CIE-LAB color space. Cleaning of the stains is expressed as percent stain removal index (% SRI) by taking a ratio between the color difference before and after washing, and comparing it to the difference of unwashed soils (before wash) to unsoiled fabric, and averaging the eight values obtained by reading two different regions of each washed swatch. Cleaning performances of PhuPro1 and Purafect® Prime HA proteases at 32° C. are shown in Tables 7.8A and  FIG. 7.8A  and at 16° C. are shown in Table 7.8B and  FIG. 7.8B . 
     
       
         
           
               
             
               
                 TABLE 7.8A 
               
               
                   
               
               
                 Cleaning performance of PhuPro1 at 32° C. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 EMPA-116 
                 EMPA-117 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Purafect Prime 
                   
                 Purafect Prime 
                   
               
               
                   
                 HA 
                 PhuPro1 
                 HA 
                 PhuPro1 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
               
               
                 ppm 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
               
               
                 enzyme 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
               
               
                   
               
               
                 0 
                 0.25 
                 0.02 
                 0.25 
                 0.02 
                 0.19 
                 0.02 
                 0.19 
                 0.02 
               
               
                 0.2 
                 0.31 
                 0.02 
                 0.31 
                 0.01 
                 0.31 
                 0.03 
                 0.32 
                 0.04 
               
               
                 0.5 
                 0.34 
                 0.02 
                 0.33 
                 0.03 
                 0.34 
                 0.02 
                 0.37 
                 0.02 
               
               
                 1 
                 0.35 
                 0.03 
                 0.36 
                 0.02 
                 0.38 
                 0.03 
                 0.42 
                 0.03 
               
               
                 1.5 
                 0.36 
                 0.02 
                 0.37 
                 0.03 
                 0.35 
                 0.03 
                 0.43 
                 0.03 
               
               
                   
               
            
           
           
               
               
               
            
               
                   
                 EMPA-112 
                 CFT C-10 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Purafect Prime 
                   
                 Purafect Prime 
                   
               
               
                   
                 HA 
                 PhuPro1 
                 HA 
                 PhuPro1 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
               
               
                 ppm 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
               
               
                 enzyme 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
               
               
                   
               
               
                 0 
                 0.15 
                 0.03 
                 0.15 
                 0.03 
                 0.07 
                 0.01 
                 0.07 
                 0.01 
               
               
                 0.2 
                 0.17 
                 0.04 
                 0.14 
                 0.02 
                 0.11 
                 0.01 
                 0.15 
                 0.01 
               
               
                 0.5 
                 0.19 
                 0.02 
                 0.19 
                 0.04 
                 0.13 
                 0.01 
                 0.16 
                 0.03 
               
               
                 1 
                 0.20 
                 0.03 
                 0.22 
                 0.03 
                 0.17 
                 0.01 
                 0.17 
                 0.01 
               
               
                 1.5 
                 0.24 
                 0.03 
                 0.25 
                 0.04 
                 0.17 
                 0.02 
                 0.20 
                 0.02 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7.8B 
               
               
                   
               
               
                 Cleaning performance of PhuPro1 at 16° C. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 EMPA-116 
                 EMPA-117 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Purafect Prime 
                   
                 Purafect Prime 
                   
               
               
                   
                 HA 
                 PhuPro1 
                 HA 
                 PhuPro1 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
               
               
                 ppm 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
               
               
                 enzyme 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
               
               
                   
               
               
                 0 
                 0.14 
                 0.02 
                 0.14 
                 0.02 
                 0 12 
                 0.01 
                 0 12 
                 0.01 
               
               
                 0.2 
                 0.19 
                 0.02 
                 0.17 
                 0.03 
                 0.17 
                 0.02 
                 0.14 
                 0.03 
               
               
                 0.5 
                 0.22 
                 0.03 
                 0.28 
                 0.04 
                 0.20 
                 0.03 
                 0.22 
                 0.01 
               
               
                 1 
                 0.24 
                 0.02 
                 0.26 
                 0.02 
                 0.20 
                 0.01 
                 0.24 
                 0.04 
               
               
                 1.5 
                 0.23 
                 0.03 
                 0.26 
                 0.03 
                 0.23 
                 0.02 
                 0.25 
                 0.02 
               
               
                   
               
            
           
           
               
               
               
            
               
                   
                 EMPA-112 
                 CFT C-10 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Purafect Prime 
                   
                 Purafect Prime 
                   
               
               
                   
                 HA 
                 PhuPro1 
                 HA 
                 PhuPro1 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95CI 
                 Average 
                 95I 
               
               
                 ppm 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
                 % SRI 
                 [% SRI 
               
               
                 enzyme 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
                 (dE) 
                 (dE)] 
               
               
                   
               
               
                 0 
                 0.09 
                 0.03 
                 0.09 
                 0.03 
                 0.07 
                 0.01 
                 0.07 
                 0.01 
               
               
                 0.2 
                 0.07 
                 0.01 
                 0.09 
                 0.02 
                 0.08 
                 0.02 
                 0.06 
                 0.01 
               
               
                 0.5 
                 0.11 
                 0.02 
                 0.12 
                 0.03 
                 0.10 
                 0.01 
                 0.09 
                 0.01 
               
               
                 1 
                 0.11 
                 0.02 
                 0.12 
                 0.02 
                 0.13 
                 0.01 
                 0.15 
                 0.01 
               
               
                 1.5 
                 0.13 
                 0.03 
                 0.19 
                 0.03 
                 0.13 
                 0.01 
                 0.11 
                 0.01 
               
               
                   
               
            
           
         
       
     
     Example 8.1 
     Cloning of  Paenibacillus amylolyticus  Metalloprotease PamPro1 
     A strain (DSM11747) of  Paenibacillus amylolyticus  was selected as a potential source of enzymes which may be useful in various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the  Paenibacillus amylolyticus  strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina&#39;s next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in  Paenibacillus amylolyticus  encodes a metalloprotease and the sequence of this gene, called PamPro1, is provided in SEQ ID NO: 36. The corresponding protein encoded by the PamPro1 gene is shown in SEQ ID NO: 37. At the N-terminus, the protein has a signal peptide with a length of 25 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PamPro1 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the  Paenibacillus polymyxa  Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PamPro1 protein is shown in SEQ ID NO: 3. 
     The nucleotide sequence of the PamPro1 gene isolated from  Paenibacillus amylolyticus  is set forth as SEQ ID NO: 36. The sequence encoding the predicted native signal peptide is shown in italics: 
     
       
         
           
               
            
               
                 
                   ATGAAATTCGCCAAAGTTATGCCAACAATTCTTGGAGGAGCTCTTTTGCTC 
                 
               
               
                   
               
               
                   GCTTCCGTATCCTCTGCTACTGCA GCTCCAGTGTCTGATCAATCCATTCCA 
               
               
                   
               
               
                 CTTCAGGCCCCTTATGCCTCTGAGGGGGGTATTCCATTGAACAGTGGAACA 
               
               
                   
               
               
                 GATGACACTA TCTTTAA TTATCTTGGACAGCAGGAACAATTTCTGAATTCC 
               
               
                   
               
               
                 GATGTGAAATCCCAGCTCAAAATTGTCAAAAGAAACACAGATACATCTGGC 
               
               
                   
               
               
                 GTAAGACACTTCCGCCTGAAACAGTATATTAAAGGTATCCCGGTTTATGGT 
               
               
                   
               
               
                 GCAGAACAGACGGTCCACCTGGACAAAACCGGAGCCGTGAGCTCCGCACTT 
               
               
                   
               
               
                 GGCGATCTTCCACCGATTGAAGAGCAGGCCATTCCGAATGATGGTGTAGCC 
               
               
                   
               
               
                 GAGATCAGCGGAGAAGACGCGATCCAGATTGCAACCGAAGAAGCAACCTCC 
               
               
                   
               
               
                 CGGATTGGAGAGCTTGGTGCCGCGGAAATCACGCCTCAAGCTGAATTGAAC 
               
               
                   
               
               
                 ATCTATCATCATGAAGAAGATGGTCAGACATA TCTGGTTTACATTACGGAA   
               
               
                   
               
               
                   GTA AACGTACTGGAACCTGCCCCTCT ACGGAC CAAATATTTCATTAACGCA 
               
               
                   
               
               
                 GTGGATGGCAGTAT CGT ATCCCAGTTTGACCTCATTAACTTCGCTACTGGA 
               
               
                   
               
               
                 ACAGGTACAGGTGTACTCGGTGATACCAAAACCCTGACAACCACCCAATCC 
               
               
                   
               
               
                 GGCAGCACCTTCCAACTGAAAGACACCACTCGTGGCAATGGCATCCAAACG 
               
               
                   
               
               
                 TATACGGCAAACAATGGCTCCTCACTGCCTGGTAGCTTGCTTACAGATTCG 
               
               
                   
               
               
                 GATAATGTATGGACCGATCGTGCAGGTGTAGATGCTCATGCTCATGCCGCT 
               
               
                   
               
               
                 GCTACGTATGATTTCTACAAAAACAAATTCAACCGTAACGGTATTAATGGT 
               
               
                   
               
               
                 AACGGATTGTTGATCAGATCAACCGTGCACTACGGCTCCAATTACAATAAC 
               
               
                   
               
               
                 GCCTTCTGGAACGGGGCACAGATTGTCTTTGGTGACGGAGATGGAACGATG 
               
               
                   
               
               
                 TTCCGATCCCTGTCTGGTGATCTGGATGTTGTGGGTCATGAATTGACGCAT 
               
               
                   
               
               
                 GGTGTTATTGAATATACAGCCAATCTGGAATATCGCAATGAACCAGGTGCA 
               
               
                   
               
               
                 CTCAATGAAGCCTTTGCCGATATTTTCGGTAATACGATCCAAAGCAAAAAC 
               
               
                   
               
               
                 TGGCTGCTCGGTGATGATATCTACACACCTAACACTCCAGGAGATGCGCTG 
               
               
                   
               
               
                 CGCTCCCTCTCCAACCCTACATTGTATGGTCAACCTGACAAATACAGCGAT 
               
               
                   
               
               
                 CGCTACACAGGCTCACAGGACAACGGCGGTGTCCATATCAACAGTGGTATC 
               
               
                   
               
               
                 ATCAATAAAGCCTATTTCCTTGCTGCTCAAGGCGGAACACATAATGGTGTG 
               
               
                   
               
               
                 ACTGTTACCGGAATCGGCCGGGATAAAGCGATCCAGATTTTCTACAGCACA 
               
               
                   
               
               
                 CTGGTGAACTACCTGACACCAACGTCCAAATTTGCCGCTGCCAAAACAGCT 
               
               
                   
               
               
                 ACCATTCAAGCAGCCAAAGATCTGTACGGAGCAACTTCCGCTGAAGCTACT 
               
               
                   
               
               
                 GCTATTACCAAAGCATATCAAGCTGTAGGCCTG 
               
            
           
         
       
     
     The amino acid sequence of the PamPro1 precursor protein is set forth as SEQ ID NO: 37. The predicted signal sequence is shown in italics, and the predicted propeptide is shown in underlined text: 
     
       
         
           
               
            
               
                 
                   MKFAKVMPTILGGALLLASVSSATA 
                   APVSDQSIPLQAPYASEGGIPLNSGT 
                 
               
               
                   
               
               
                 
                   DDTIFNYLGQQEQFLNSDVKSQLKIVKRNTDTSGVRHFRLKQYIKGIPVYG 
                 
               
               
                   
               
               
                 
                   AEQTVHLDKTGAVSSALGDLPPIEEQAIPNDGVAEISGEDAIQIATEEATS 
                 
               
               
                   
               
               
                 
                   RIGELGAAEITPQAELNIYHHEEDGQTYLVYITEVNVLEPAPLRTKYFINA 
                 
               
               
                   
               
               
                   VDGSIVSQFDLINF ATGTGTGVLGDTKTLTTTQSGSTFQLKDTTRGNGIQT 
               
               
                   
               
               
                 YTANNGSSLPGSLLTDSDNVWTDRAGVDAHAHAAATYDFYKNKFNRNGING 
               
               
                   
               
               
                 NGLLIRSTVHYGSNYNNAFWNGAQIVFGDGDGTMFRSLSGDLDVVGHELTH 
               
               
                   
               
               
                 GVIEYTANLEYRNEPGALNEAFADIFGNTIQSKNWLLGDDIYTPNTPGDAL 
               
               
                   
               
               
                 RSLSNPTLYGQPDKYSDRYTGSQDNGGVHINSGIINKAYFLAAQGGTHNGV 
               
               
                   
               
               
                 TVTGIGRDKAIQIFYSTLVNYLTPTSKFAAAKTATIQAAKDLYGATSAEAT 
               
               
                   
               
               
                 AITKAYQAVGL 
               
            
           
         
       
     
     The amino acid sequence of the predicted mature form of PamPro1 is set forth as SEQ ID NO: 38: 
     
       
         
           
               
            
               
                 ATGTGTGVLGDTKTLTTTQSGSTFQLKDTTRGNGIQTYTANNGSSLPGSLL 
               
               
                   
               
               
                 TDSDNVWTDRAGVDAHAHAAATYDFYKNKFNRNGINGNGLLIRSTVHYGSN 
               
               
                   
               
               
                 YNNAFWNGAQIVFGDGDGTMFRSLSGDLDVVGHELTHGVIEYTANLEYRNE 
               
               
                   
               
               
                 PGALNEAFADIFGNTIQSKNWLLGDDIYTPNTPGDALRSLSNPTLYGQPDK 
               
               
                   
               
               
                 YSDRYTGSQDNGGVHINSGIINKAYFLAAQGGTHNGVTVTGIGRDKAIQIF 
               
               
                   
               
               
                 YSTLVNYLTPTSKFAAAKTATIQAAKDLYGATSAEATAITKAYQAVGL 
               
            
           
         
       
     
     Example 8.2 
     Expression of  Paenibacillus amylolyticus  Metalloprotease PamPro1 
     The DNA sequence of the propeptide-mature form of PamPro1 was synthesized and inserted into the  Bacillus subtilis  expression vector p2JM103BBI (Vogtentanz,  Protein Expr Purif,  55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX146(AprE-PamPro1) ( FIG. 1 ). Ligation of this gene encoding the PamPro1 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3′ end of the  B. subtilis  AprE signal sequence and the 5′ end of the predicted PamPro1 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in  FIG. 8.1 , labeled pGX146(AprE-PamPro1) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in  B. subtilis , and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PamPro1 (SEQ ID NO: 39). The translation product of the synthetic AprE-PamPro1 gene is shown in SEQ ID NO: 40. 
     The pGX146(AprE-PamPro1) plasmid was then transformed into  B. subtilis  cells (degU Hy  32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat #232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl 2 ). 
     The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl 2  and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to an 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above; and the active flow-through fractions were collected and concentrated. The sample was loaded onto a 320 ml Superdex 75 gel filtration column pre-equilibrated with the loading buffer described above containing 0.15 M NaCl. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses. 
     The nucleotide sequence of the synthesized PamPro1 gene in plasmid pGX146(AprE-PamPro1) is depicted in SEQ ID NO: 39. The sequence encoding the three residue addition (AGK) is shown in bold: 
     
       
         
           
               
            
               
                 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAATC 
               
               
                   
               
               
                 TTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCT GCTGGAAAAG CTCCG 
               
               
                   
               
               
                 GTTAGCGACCAGTCAATCCCTCTTCAAGCACCGTATGCCAGCGAAGGAGGC 
               
               
                   
               
               
                 ATTCCGCTTAACAGCGGCACGGACGACACGATTTTCAATTACCTGGGCCAA 
               
               
                   
               
               
                 CAGGAGCAGTTCCTGAACAGCGACGTCAAGAGCCAGCTGAAGATCGTCAAA 
               
               
                   
               
               
                 AGAAACACAGACACATCAGGCGTGAGACACTTCAGACTGAAGCAATACATC 
               
               
                   
               
               
                 AAGGGCATCCCGGTTTATGGCGCTGAACAAACGGTTCACCTGGACAAAACA 
               
               
                   
               
               
                 GGCGCAGTTTCATCAGCACTGGGAGATCTGCCGCCGATTGAAGAGCAAGCA 
               
               
                   
               
               
                 ATCCCGAATGATGGAGTTGCGGAAATTAGCGGCGAGGATGCAATCCAAATC 
               
               
                   
               
               
                 GCGACGGAGGAGGCTACATCAAGAATTGGAGAACTTGGCGCAGCGGAGATT 
               
               
                   
               
               
                 ACACCGCAGGCTGAACTGAACATCTATCACCATGAGGAAGACGGCCAGACG 
               
               
                   
               
               
                 TACCTGGTTTACATTACGGAAGTGAACGTGCTGGAACCGGCACCTCTGAGA 
               
               
                   
               
               
                 ACAAAGTACTTTATCAACGCGGTTGACGGCAGCATCGTCTCACAGTTCGAC 
               
               
                   
               
               
                 CTGATTAACTTCGCCACGGGAACAGGAACGGGCGTTCTTGGAGACACAAAG 
               
               
                   
               
               
                 ACGCTGACGACGACGCAGTCAGGCAGCACATTCCAGCTGAAGGACACAACA 
               
               
                   
               
               
                 AGAGGCAACGGCATCCAAACGTACACGGCGAACAATGGATCATCACTGCCG 
               
               
                   
               
               
                 GGCTCACTGCTGACGGATTCAGATAACGTGTGGACGGATAGAGCTGGCGTT 
               
               
                   
               
               
                 GACGCGCATGCTCACGCTGCTGCGACGTACGACTTCTACAAGAACAAGTTC 
               
               
                   
               
               
                 AACAGAAACGGCATTAACGGAAATGGCCTGCTGATCAGAAGCACGGTGCAT 
               
               
                   
               
               
                 TATGGCTCAAACTACAACAACGCTTTTTGGAACGGCGCACAGATCGTGTTT 
               
               
                   
               
               
                 GGCGACGGCGATGGCACAATGTTTAGAAGCCTGTCAGGAGACCTGGATGTG 
               
               
                   
               
               
                 GTGGGCCACGAACTGACGCACGGCGTGATCGAGTATACGGCGAACCTTGAA 
               
               
                   
               
               
                 TATAGAAACGAGCCGGGAGCACTGAATGAGGCGTTCGCGGACATTTTCGGC 
               
               
                   
               
               
                 AACACAATCCAGAGCAAAAACTGGCTGCTGGGCGACGATATCTATACACCG 
               
               
                   
               
               
                 AACACACCGGGCGATGCACTGAGATCACTGTCAAATCCGACGCTGTATGGC 
               
               
                   
               
               
                 CAACCGGATAAGTACTCAGACAGATATACGGGCAGCCAAGACAATGGCGGC 
               
               
                   
               
               
                 GTTCACATCAACTCAGGCATCATCAACAAGGCTTACTTCCTTGCGGCCCAA 
               
               
                   
               
               
                 GGAGGAACACATAACGGCGTTACAGTTACAGGCATTGGCAGAGACAAGGCG 
               
               
                   
               
               
                 ATCCAGATCTTTTACAGCACGCTGGTGAACTACCTGACACCTACGTCAAAG 
               
               
                   
               
               
                 TTTGCCGCAGCGAAAACAGCAACAATTCAGGCGGCTAAAGACCTGTACGGA 
               
               
                   
               
               
                 GCGACATCAGCCGAGGCCACAGCAATTACAAAAGCATATCAAGCAGTTGGC 
               
               
                   
               
               
                 CTTTAA 
               
            
           
         
       
     
     The amino acid sequence of the PamPro1 precursor protein expressed from plasmid pGX146(AprE-PamPro1) is depicted in SEQ ID NO: 40. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text. 
     
       
         
           
               
            
               
                 
                   MRSKKLWISLLFALTLIFTMAFSNMSAQA 
                   AGK 
                   APVSDQSIPLQAPYASEGG 
                 
               
               
                   
               
               
                 
                   IPLNSGTDDTIFNYLGQQEQFLNSDVKSQLKIVKRNTDTSGVRHFRLKQYI 
                 
               
               
                   
               
               
                 
                   KGIPVYGAEQTVHLDKTGAVSSALGDLPPIEEQAIPNDGVAEISGEDAIQI 
                 
               
               
                   
               
               
                 
                   ATEEATSRIGELGAAEITPQAELNIYHHEEDGQTYLVYITEVNVLEPAPLR 
                 
               
               
                   
               
               
                   TKYFINAVDGSIVSQFDLINF ATGTGTGVLGDTKTLTTTQSGSTFQLKDTT 
               
               
                   
               
               
                 RGNGIQTYTANNGSSLPGSLLTDSDNVWTDRAGVDAHAHAAATYDFYKNKE 
               
               
                   
               
               
                 NRNGINGNGLLIRSTVHYGSNYNNAFWNGAQIVFGDGDGTMFRSLSGDLDV 
               
               
                   
               
               
                 VGHELTHGVIEYTANLEYRNEPGALNEAFADIFGNTIQSKNWLLGDDIYTP 
               
               
                   
               
               
                 NTPGDALRSLSNPTLYGQPDKYSDRYTGSQDNGGVHINSGIINKAYFLAAQ 
               
               
                   
               
               
                 GGTHNGVTVTGIGRDKAIQIFYSTLVNYLTPTSKFAAAKTATIQAAKDLYG 
               
               
                   
               
               
                 ATSAEATAITKAYQAVGL 
               
            
           
         
       
     
     Example 8.3 
     Proteolytic Activity of Metalloprotease PamPro1 
     The proteolytic activity of purified metalloprotease PamPro1 was measured in 50 mM Tris (pH 7), using azo-casein (Cat #74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H 2 O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A 440 ) using a SpectraMax 190. Net A 440  was calculated by subtracting the A 440  of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of triplicate assays. The proteolytic activity is shown as Net A 44 o. The proteolytic assay with azo-casein as the substrate (shown in  FIG. 8.2 ) indicates that PamPro1 is an active protease. 
     Example 8.4 
     pH Profiles of Metalloprotease PamPro1 
     With azo-casein as the substrate, the pH profiles of metalloprotease PamPro1 were studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl Milli-Q H 2 O diluted enzyme (125 ppm) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 8.3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in  FIG. 8.3 , the optimal pH of PamPro1 is about 8, with greater than 70% of maximal activity retained between 7 and 9.5. 
     Example 8.5 
     Temperature Profile of Metalloprotease PamPro1 
     The temperature profile of metalloprotease PamPro1 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assays. The enzyme sample and azo-casein substrate were prepared as in Example 8.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H 2 O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20-90° C.) for 5 min. After the incubation, 5 μl of diluted enzyme (50 ppm) or H 2 O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 8.3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in  FIG. 8.4  suggest that PamPro1 showed an optimal temperature at about 50° C., and retained greater than 70% of its maximum activity between 45 and 55° C. 
     Example 8.6 
     Cleaning Performance of Metalloprotease PamPro1 
     The cleaning performance of PamPro1 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified protease samples were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl 2 , 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca 2+ :Mg 2+ =3:1) (detergent composition shown in Table 8.1). To initiate the reaction, 180 μl of the AT detergent buffered at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the “Initial performance”) using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as the “Wash-off”). The sum of two absorbance measurements (“Initial performance” plus “Wash-off”) gives the “Total performance”, which measures the protease activity on the model stain; and Net A 405  was subsequently calculated by subtracting the A 405  of the “Total performance” of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 in AT dish detergent for PamPro1 is shown in  FIGS. 5A and 5B . 
     
       
         
           
               
             
               
                 TABLE 8.1 
               
             
            
               
                   
               
               
                 Composition of AT dish detergent formula with bleach 
               
            
           
           
               
               
            
               
                 Ingredient 
                 Concentration (mg/ml) 
               
               
                   
               
            
           
           
               
               
            
               
                 MGDA (methylglycinediacetic acid) 
                 0.143 
               
               
                 Sodium citrate 
                 1.86 
               
               
                 Citric acid* 
                 varies 
               
               
                 PAP (peracid N,N- 
                 0.057 
               
               
                 phthaloylaminoperoxycaproic acid) 
                   
               
               
                 Plurafac ® LF 18B (a non-ionic surfactant) 
                 0.029 
               
               
                 Bismuthcitrate 
                 0.006 
               
               
                 Bayhibit ® S (Phosphonobutantricarboxylic 
                 0.006 
               
               
                 acid sodium salt) 
                   
               
               
                 Acusol ™ 587 (a calcium polyphosphate 
                 0.029 
               
               
                 inhibitor) 
                   
               
               
                 PEG 6000 
                 0.043 
               
               
                 PEG 1500 
                 0.1 
               
               
                   
               
               
                 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid. 
               
            
           
         
       
     
     Example 8.7 
     Comparison of PamPro1 to Other Proteases 
     A. Identification of Homologous Proteases 
     Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The predicted mature protein amino acid sequence for PamPro1 (SEQ ID NO: 38) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 8.2A and 8.2B provide a list of sequences with the percent identity to PamPro1. The length in Table 8.2 refers to the entire sequence length of the homologous proteases. 
     
       
         
           
               
             
               
                 TABLE 8.2A 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PamPro1 protein identified 
               
               
                 from the NCBI non-redundant protein database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Accession # 
                 PamPro1 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 P23384 
                 56 
                 
                   Bacillus caldolyticus 
                 
                 544 
               
               
                 P00800 
                 56 
                 
                   Bacillus thermoproteolyticus 
                 
                 548 
               
               
                 ZP_08640523.1 
                 57 
                 
                   Brevibacillus laterosporus 
                 
                 564 
               
               
                   
                   
                 LMG 15441 
                   
               
               
                 BAA06144.1 
                 57 
                   Lactobacillus  sp. 
                 566 
               
               
                 YP_003872180.1 
                 58 
                   Paenibacillus polymyxa  E681 
                 587 
               
               
                 ZP_04149724.1 
                 59 
                 
                   Bacillus pseudomycoides 
                 
                 566 
               
               
                   
                   
                 DSM 12442 
                   
               
               
                 EJR46541.1 
                 60 
                   Bacillus cereus  VD107 
                 566 
               
               
                 YP_001373863.1 
                 60 
                   Bacillus cytotoxicus  NVH 391-98 
                 565 
               
               
                 ZP_10738945.1 
                 61 
                   Brevibacillus  sp. CF112 
                 528 
               
               
                 YP_004646155.1 
                 61 
                 
                   Paenibacillus mucilaginosus 
                 
                 525 
               
               
                   
                   
                 KNP414 
                   
               
               
                 ZP_02326602.1 
                 62 
                   Paenibacillus larvae  subsp. 
                 520 
               
               
                   
                   
                   larvae  BRL-230010 
                   
               
               
                 P43263 
                 63 
                 
                   Brevibacillus brevis 
                 
                 527 
               
               
                 ZP_09775365.1 
                 64 
                   Paenibacillus  sp. Aloe-11 
                 580 
               
               
                 ZP_09077634.1 
                 65 
                   Paenibacillus elgii  B69 
                 529 
               
               
                 ZP_09071078.1 
                 68 
                   Paenibacillus larvae  subsp.  
                 529 
               
               
                   
                   
                   larvae  B-3650 
                   
               
               
                 ZP_08511445.1 
                 69 
                   Paenibacillus  sp. HGF7 
                 525 
               
               
                 YP_005073223.1 
                 70 
                   Paenibacillus terrae  HPL-003 
                 591 
               
               
                 YP_003948511.1 
                 71 
                   Paenibacillus polymyxa  SC2 
                 592 
               
               
                 ZP_10241030.1 
                 71 
                   Paenibacillus peoriae  KCTC 3763 
                 593 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 8.2B 
               
             
            
               
                   
               
               
                 List of sequences with percent identity to PamPro1 protein identified  
               
               
                 from the Genome Quest Patent database 
               
            
           
           
               
               
               
               
            
               
                   
                 PID to 
                   
                   
               
               
                 Patent # 
                 PamPro1 
                 Organism 
                 Length 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 US7335504-0030 
                 56.63 
                 
                   Bacillus thermoproteolyticus 
                 
                 316 
               
               
                 US20120107907-0184 
                 56.91 
                 
                   Bacillus caldoyticus 
                 
                 319 
               
               
                 JP2006124323-0003 
                 56.96 
                 
                   Bacillus thermoproteolyticus 
                 
                 316 
               
               
                 JP1993199872-0001 
                 56.96 
                   Bacillus  sp. 
                 316 
               
               
                 JP1997000255-0001 
                 56.96 
                 empty 
                 548 
               
               
                 US6518054-0001 
                 57.23 
                   Bacillus  sp. 
                 319 
               
               
                 US20120107907-0176 
                 57.23 
                 
                   Bacillus stearothermophilis 
                 
                 548 
               
               
                 US8114656-0183 
                 57.28 
                 
                   Bacillus stearothermophilis 
                 
                 316 
               
               
                 US20120009651-0002 
                 57.28 
                 
                   Geobacillus  
                 
                 548 
               
               
                   
                   
                 
                   caldoproteolyticus 
                 
                   
               
               
                 JP2011103791-0020 
                 57.28 
                 
                   Geobacillus  
                 
                 552 
               
               
                   
                   
                 
                   stearothermophilus 
                 
                   
               
               
                 WO2012110562-0006 
                 57.88 
                 
                   Bacillus megaterium 
                 
                 320 
               
               
                 EP2390321-0178 
                 57.88 
                 
                   Bacillus thuringiensis 
                 
                 566 
               
               
                 US6518054-0002 
                 57.93 
                   Bacillus  sp. 
                 316 
               
               
                 WO2012110562-0007 
                 58.25 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 JP1995184649-0001 
                 58.52 
                   Lactobacillus  sp. 
                 566 
               
               
                 EP2178896-0184 
                 58.52 
                 
                   Bacillus anthracis 
                 
                 566 
               
               
                 EP2390321-0195 
                 59.55 
                 
                   Bacillus cereus 
                 
                 317 
               
               
                 WO2012110563-0005 
                 59.87 
                 
                   Bacillus cereus 
                 
                 320 
               
               
                 US20080293610-0186 
                 63.25 
                 
                   Bacillus brevis 
                 
                 304 
               
               
                 JP2005229807-0018 
                 71.19 
                 
                   Paenibacillus polymyxa 
                 
                 566 
               
               
                 US8114656-0187 
                 71.43 
                 
                   Bacillus polymyxa 
                 
                 302 
               
               
                   
               
            
           
         
       
     
     B. Alignment of Homologous Protease Sequences 
     The amino acid sequence of the predicted mature PamPro1 (SEQ ID NO: 38) was aligned with thermolysin (P00800,  Bacillus thermoproteolyticus ), and protease from  Paenibacillus peoriae  KCTC 3763 (YP 005073223.1) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters.  FIG. 8.6  shows the alignment of PamPro1 with these protease sequences. 
     C. Phylogenetic Tree 
     A phylogenetic tree for full length sequences of PamPro1 (SEQ ID NO: 37) was built using sequences of representative homologs from Table 8.2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. MolBiol.Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in  FIG. 8.7 . 
     Example 9 
     Comparison of the Various  Paenibacillus  Metalloproteases with Other Bacterial Metalloprotease Homologs 
     A. Alignment of Homologous Protease Sequences 
     The amino acid sequence of the predicted mature sequences for the  Paenibacillus  proteases described in Examples 1.1 to 8.7 were aligned with related bacterial metalloproteases using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters.  FIG. 9.1  shows the alignment of the various  Paenibacillus  metalloproteases with other bacterial metalloprotease homologs. 
     B. Phylogenetic Tree 
     A phylogenetic tree for full length sequences of the metalloproteases aligned in  FIG. 9.1  was created using the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. MolBiol.Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in  FIG. 9.2 , where one can observe the clustering of the sequences from  Paenibacillus  genus. 
     While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein can be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.