Patent Publication Number: US-11643657-B2

Title: Chiral control

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is a Division of application Ser. No. 15/462,787, filed on Mar. 17, 2017, issued as U.S. Pat. No. 10,590,413, which is a Division of application Ser. No. 14/414,614, filed on Jan. 13, 2015, issued as U.S. Pat. No. 9,982,257, which is a 371 National Stage Application of PCT International Application PCT/US13/50407, filed Jul. 12, 2013, which claims priority to U.S. Provisional Application Ser. No. 61/671,655, filed Jul. 13, 2012, 61/671,656, filed Jul. 13, 2012, 61/671,722, filed Jul. 14, 2012, and 61/671,724, filed Jul. 14, 2012, the entirety of each of which is incorporated herein by reference. 
    
    
     SEQUENCE LISTING 
     In accordance with 37 CFR 1.52(e)(5), a Sequence Listing in the form of a text file (entitled “SequenceListing.txt,” created on Aug. 19, 2013, and 531 kilobytes) is incorporated herein by reference in its entirety. 
     BACKGROUND OF THE INVENTION 
     Oligonucleotides are useful in therapeutic, diagnostic, research and nanomaterials applications. The use of naturally occurring nucleic acids (e.g., unmodified DNA or RNA) for therapeutics can be limited, for example, because of their instability against extra- and intracellular nucleases and/or their poor cell penetration and distribution. Additionally, in vitro studies have shown that properties of antisense oligonucleotides such as binding affinity, sequence specific binding to the complementary RNA (Cosstick and Eckstein, 1985; LaPlanche et al., 1986; Latimer et al., 1989; Hacia et al., 1994; Mesmaeker et al., 1995), and stability to nucleases can be affected by the absolute stereochemical configurations of the phosphorus atoms (Cook, et al. US005599797A). Therefore, there is a need for new and improved oligonucleotide compositions. 
     SUMMARY OF THE INVENTION 
     The present invention encompasses the recognition that there exists a need for chirally controlled oligonucleotide compositions and new methods for synthesizing the same. The invention specifically encompasses the identification of the source of certain problems with prior methodologies for preparing chiral oligonucleotides, including problems that prohibit preparation of fully chirally controlled compositions, particularly compositions comprising a plurality of oligonucleotide types. 
     In some embodiments, the present invention provides chirally controlled oligonucleotide compositions. 
     In some embodiments, the present invention provides methods of making chirally controlled oligonucleotides and chirally controlled oligonucleotide compositions. 
     In some embodiments, the present invention provides methods of using chirally controlled oligonucleotide and chirally controlled oligonucleotide compositions. 
     All publications and patent documents cited in this application are incorporated herein by reference in their entirety. 
     Definitions 
     Aliphatic: The term “aliphatic” or “aliphatic group”, as used herein, means a straight-chain (i.e., unbranched) or branched, substituted or unsubstituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation, or a monocyclic hydrocarbon or bicyclic or polycyclic hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic (also referred to herein as “carbocycle” “cycloaliphatic” or “cycloalkyl”), that has a single point of attachment to the rest of the molecule. In some embodiments, aliphatic groups contain 1-50 aliphatic carbon atoms. Unless otherwise specified, aliphatic groups contain 1-10 aliphatic carbon atoms. In some embodiments, aliphatic groups contain 1-6 aliphatic carbon atoms. In some embodiments, aliphatic groups contain 1-5 aliphatic carbon atoms. In other embodiments, aliphatic groups contain 1-4 aliphatic carbon atoms. In still other embodiments, aliphatic groups contain 1-3 aliphatic carbon atoms, and in yet other embodiments, aliphatic groups contain 1-2 aliphatic carbon atoms. In some embodiments, “cycloaliphatic” (or “carbocycle” or “cycloalkyl”) refers to a monocyclic or bicyclic C 3 -C 10  hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule. In some embodiments, “cycloaliphatic” (or “carbocycle” or “cycloalkyl”) refers to a monocyclic C 3 -C 6  hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule. Suitable aliphatic groups include, but are not limited to, linear or branched, substituted or unsubstituted alkyl, alkenyl, alkynyl groups and hybrids thereof such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl or (cycloalkyl)alkenyl. 
     Alkylene: The term “alkylene” refers to a bivalent alkyl group. An “alkylene chain” is a polymethylene group, i.e., —(CH 2 ) n —, wherein n is a positive integer, preferably from 1 to 6, from 1 to 4, from 1 to 3, from 1 to 2, or from 2 to 3. A substituted alkylene chain is a polymethylene group in which one or more methylene hydrogen atoms are replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group. 
     Alkenylene: The term “alkenylene” refers to a bivalent alkenyl group. A substituted alkenylene chain is a polymethylene group containing at least one double bond in which one or more hydrogen atoms are replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group. 
     Animal: As used herein, the term “animal” refers to any member of the animal kingdom. In some embodiments, “animal” refers to humans, at any stage of development. In some embodiments, “animal” refers to non-human animals, at any stage of development. In certain embodiments, the non-human animal is a mammal (e.g., a rodent, a mouse, a rat, a rabbit, a monkey, a dog, a cat, a sheep, cattle, a primate, and/or a pig). In some embodiments, animals include, but are not limited to, mammals, birds, reptiles, amphibians, fish, and/or worms. In some embodiments, an animal may be a transgenic animal, a genetically-engineered animal, and/or a clone. 
     Approximately: As used herein, the terms “approximately” or “about” in reference to a number are generally taken to include numbers that fall within a range of 5%, 10%, 15%, or 20% in either direction (greater than or less than) of the number unless otherwise stated or otherwise evident from the context (except where such number would be less than 0% or exceed 100% of a possible value). In some embodiments, use of the term “about” in reference to dosages means±5 mg/kg/day. 
     Aryl: The term “aryl” used alone or as part of a larger moiety as in “aralkyl,” “aralkoxy,” or “aryloxyalkyl,” refers to monocyclic and bicyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic and wherein each ring in the system contains three to seven ring members. The term “aryl” may be used interchangeably with the term “aryl ring.” In certain embodiments of the present invention, “aryl” refers to an aromatic ring system which includes, but not limited to, phenyl, biphenyl, naphthyl, anthracyl and the like, which may bear one or more substituents. Also included within the scope of the term “aryl,” as it is used herein, is a group in which an aromatic ring is fused to one or more non-aromatic rings, such as indanyl, phthalimidyl, naphthimidyl, phenanthridinyl, or tetrahydronaphthyl, and the like. 
     Characteristic portion: As used herein, the phrase a “characteristic portion” of a protein or polypeptide is one that contains a continuous stretch of amino acids, or a collection of continuous stretches of amino acids, that together are characteristic of a protein or polypeptide. Each such continuous stretch generally will contain at least two amino acids. Furthermore, those of ordinary skill in the art will appreciate that typically at least 5, 10, 15, 20 or more amino acids are required to be characteristic of a protein. In general, a characteristic portion is one that, in addition to the sequence identity specified above, shares at least one functional characteristic with the relevant intact protein. 
     Characteristic sequence: A “characteristic sequence” is a sequence that is found in all members of a family of polypeptides or nucleic acids, and therefore can be used by those of ordinary skill in the art to define members of the family. 
     Characteristic structural element: The term “characteristic structural element” refers to a distinctive structural element (e.g., core structure, collection of pendant moieties, sequence element, etc) that is found in all members of a family of polypeptides, small molecules, or nucleic acids, and therefore can be used by those of ordinary skill in the art to define members of the family. 
     Comparable: The term “comparable” is used herein to describe two (or more) sets of conditions or circumstances that are sufficiently similar to one another to permit comparison of results obtained or phenomena observed. In some embodiments, comparable sets of conditions or circumstances are characterized by a plurality of substantially identical features and one or a small number of varied features. Those of ordinary skill in the art will appreciate that sets of conditions are comparable to one another when characterized by a sufficient number and type of substantially identical features to warrant a reasonable conclusion that differences in results obtained or phenomena observed under the different sets of conditions or circumstances are caused by or indicative of the variation in those features that are varied. 
     Dosing regimen: As used herein, a “dosing regimen” or “therapeutic regimen” refers to a set of unit doses (typically more than one) that are administered individually to a subject, typically separated by periods of time. In some embodiments, a given therapeutic agent has a recommended dosing regimen, which may involve one or more doses. In some embodiments, a dosing regimen comprises a plurality of doses each of which are separated from one another by a time period of the same length; in some embodiments, a dosing regime comprises a plurality of doses and at least two different time periods separating individual doses. In some embodiments, all doses within a dosing regimen are of the same unit dose amount. In some embodiments, different doses within a dosing regimen are of different amounts. In some embodiments, a dosing regimen comprises a first dose in a first dose amount, followed by one or more additional doses in a second dose amount different from the first dose amount. In some embodiments, a dosing regimen comprises a first dose in a first dose amount, followed by one or more additional doses in a second dose amount same as the first dose amount. 
     Equivalent agents: Those of ordinary skill in the art, reading the present disclosure, will appreciate that the scope of useful agents in the context of the present invention is not limited to those specifically mentioned or exemplified herein. In particular, those skilled in the art will recognize that active agents typically have a structure that consists of a core and attached pendant moieties, and furthermore will appreciate that simple variations of such core and/or pendant moieties may not significantly alter activity of the agent. For example, in some embodiments, substitution of one or more pendant moieties with groups of comparable three-dimensional structure and/or chemical reactivity characteristics may generate a substituted compound or portion equivalent to a parent reference compound or portion. In some embodiments, addition or removal of one or more pendant moieties may generate a substituted compound equivalent to a parent reference compound. In some embodiments, alteration of core structure, for example by addition or removal of a small number of bonds (typically not more than 5, 4, 3, 2, or 1 bonds, and often only a single bond) may generate a substituted compound equivalent to a parent reference compound. In many embodiments, equivalent compounds may be prepared by methods illustrated in general reaction schemes as, for example, described below, or by modifications thereof, using readily available starting materials, reagents and conventional or provided synthesis procedures. In these reactions, it is also possible to make use of variants, which are in themselves known, but are not mentioned here. 
     Equivalent Dosage: The term “equivalent dosage” is used herein to compare dosages of different pharmaceutically active agents that effect the same biological result. Dosages of two different agents are considered to be “equivalent” to one another in accordance with the present invention if they achieve a comparable level or extent of the biological result. In some embodiments, equivalent dosages of different pharmaceutical agents for use in accordance with the present invention are determined using in vitro and/or in vivo assays as described herein. In some embodiments, one or more lysosomal activating agents for use in accordance with the present invention is utilized at a dose equivalent to a dose of a reference lysosomal activating agent; in some such embodiments, the reference lysosomal activating agent for such purpose is selected from the group consisting of small molecule allosteric activators (e.g., pyrazolpyrimidines), imminosugars (e.g., isofagomine), antioxidants (e.g., n-acetyl-cysteine), and regulators of cellular trafficking (e.g., Rab1a polypeptide). 
     Heteroaliphatic: The term “heteroaliphatic” refers to an aliphatic group wherein one or more units selected from C, CH, CH 2 , or CH 3  are independently replaced by a heteroatom. In some embodiments, a heteroaliphatic group is heteroalkyl. In some embodiments, a heteroaliphatic group is heteroalkenyl. 
     Heteroaryl: The terms “heteroaryl” and “heteroar-,” used alone or as part of a larger moiety, e.g., “heteroaralkyl,” or “heteroaralkoxy,” refer to groups having 5 to 10 ring atoms, preferably 5, 6, or 9 ring atoms; having 6, 10, or 14 π electrons shared in a cyclic array; and having, in addition to carbon atoms, from one to five heteroatoms. The term “heteroatom” refers to nitrogen, oxygen, or sulfur, and includes any oxidized form of nitrogen or sulfur, and any quaternized form of a basic nitrogen. Heteroaryl groups include, without limitation, thienyl, furanyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolizinyl, purinyl, naphthyridinyl, and pteridinyl. The terms “heteroaryl” and “heteroar-,” as used herein, also include groups in which a heteroaromatic ring is fused to one or more aryl, cycloaliphatic, or heterocyclyl rings, where the radical or point of attachment is on the heteroaromatic ring. Nonlimiting examples include indolyl, isoindolyl, benzothienyl, benzofuranyl, dibenzofuranyl, indazolyl, benzimidazolyl, benzthiazolyl, quinolyl, isoquinolyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, 4H-quinolizinyl, carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, and pyrido[2,3-b]-1,4-oxazin-3(4H)-one. A heteroaryl group may be mono- or bicyclic. The term “heteroaryl” may be used interchangeably with the terms “heteroaryl ring,” “heteroaryl group,” or “heteroaromatic,” any of which terms include rings that are optionally substituted. The term “heteroaralkyl” refers to an alkyl group substituted by a heteroaryl, wherein the alkyl and heteroaryl portions independently are optionally substituted. 
     Heteroatom: The term “heteroatom” means one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3,4-dihydro-2H-pyrrolyl), NH (as in pyrrolidinyl) or NR +  (as in N-substituted pyrrolidinyl)). 
     Heterocycle: As used herein, the terms “heterocycle,” “heterocyclyl,” “heterocyclic radical,” and “heterocyclic ring” are used interchangeably and refer to a stable 3- to 7-membered monocyclic or 7-10-membered bicyclic heterocyclic moiety that is either saturated or partially unsaturated, and having, in addition to carbon atoms, one or more, preferably one to four, heteroatoms, as defined above. When used in reference to a ring atom of a heterocycle, the term “nitrogen” includes a substituted nitrogen. As an example, in a saturated or partially unsaturated ring having 0-3 heteroatoms selected from oxygen, sulfur or nitrogen, the nitrogen may be N (as in 3,4-dihydro-2H-pyrrolyl), NH (as in pyrrolidinyl), or NR (as in N-substituted pyrrolidinyl). 
     A heterocyclic ring can be attached to its pendant group at any heteroatom or carbon atom that results in a stable structure and any of the ring atoms can be optionally substituted. Examples of such saturated or partially unsaturated heterocyclic radicals include, without limitation, tetrahydrofuranyl, tetrahydrothiophenyl pyrrolidinyl, piperidinyl, pyrrolinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, oxazolidinyl, piperazinyl, dioxanyl, dioxolanyl, diazepinyl, oxazepinyl, thiazepinyl, morpholinyl, and quinuclidinyl. The terms “heterocycle,” “heterocyclyl,” “heterocyclyl ring,” “heterocyclic group,” “heterocyclic moiety,” and “heterocyclic radical,” are used interchangeably herein, and also include groups in which a heterocyclyl ring is fused to one or more aryl, heteroaryl, or cycloaliphatic rings, such as indolinyl, 3H-indolyl, chromanyl, phenanthridinyl, or tetrahydroquinolinyl, where the radical or point of attachment is on the heterocyclyl ring. A heterocyclyl group may be mono- or bicyclic. The term “heterocyclylalkyl” refers to an alkyl group substituted by a heterocyclyl, wherein the alkyl and heterocyclyl portions independently are optionally substituted. 
     Intraperitoneal: The phrases “intraperitoneal administration” and “administered intraperitonealy” as used herein have their art-understood meaning referring to administration of a compound or composition into the peritoneum of a subject. 
     In vitro: As used herein, the term “in vitro” refers to events that occur in an artificial environment, e.g., in a test tube or reaction vessel, in cell culture, etc., rather than within an organism (e.g., animal, plant, and/or microbe). 
     In vivo: As used herein, the term “in vivo” refers to events that occur within an organism (e.g., animal, plant, and/or microbe). 
     Lower alkyl: The term “lower alkyl” refers to a C 1-4  straight or branched alkyl group. Exemplary lower alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, isobutyl, and tert-butyl. 
     Lower haloalkyl: The term “lower haloalkyl” refers to a C 1-4  straight or branched alkyl group that is substituted with one or more halogen atoms. 
     Optionally substituted: As described herein, compounds of the invention may contain “optionally substituted” moieties. In general, the term “substituted,” whether preceded by the term “optionally” or not, means that one or more hydrogens of the designated moiety are replaced with a suitable substituent. Unless otherwise indicated, an “optionally substituted” group may have a suitable substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position. Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds. The term “stable,” as used herein, refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, and, in certain embodiments, their recovery, purification, and use for one or more of the purposes disclosed herein. 
     Suitable monovalent substituents on a substitutable carbon atom of an “optionally substituted” group are independently halogen; —(CH 2 ) 0-4 R ∘ ; —(CH 2 ) 0-4 OR ∘ ; —O(CH 2 ) 0-4 R ∘ , —O—(CH 2 ) 0-4 C(O)OR ∘ ; —(CH 2 ) 0-4 CH(OR ∘ ) 2 ; —(CH 2 ) 0-4 SR ∘ ; —(CH 2 ) 0-4 Ph, which may be substituted with R ∘ ; —(CH 2 ) 0-4 O(CH 2 ) 0-1 Ph which may be substituted with R ∘ ; —CH═CHPh, which may be substituted with R ∘ ; —(CH 2 ) 0-4 O(CH 2 ) 0-1 -pyridyl which may be substituted with R ∘ ; —NO 2 ; —CN; —N 3 ; —(CH 2 ) 0-4 N(R ∘ ) 2 ; —(CH 2 ) 0-4 N(R ∘ )C(O)R ∘ ; —N(R ∘ )C(S)R ∘ ; —(CH 2 ) 0-4 N(R ∘ )C(O)NR ∘   2 ; —N(R ∘ )C(S)NR ∘   2 ; —(CH 2 ) 0-4 N(R ∘ )C(O)OR ∘ ; —N(R ∘ )N(R ∘ )C(O)R ∘ ; —N(R ∘ )N(R ∘ )C(O)NR ∘   2 ; —N(R ∘ )N(R ∘ )C(O)OR ∘ ; —(CH 2 ) 0-4 C(O)R ∘ ; —C(S)R ∘ ; —(CH 2 ) 0-4 C(O)OR ∘ ; —(CH 2 ) 0-4 C(O)SR ∘ ; —(CH 2 ) 0-4 C(O)OSiR ∘   3 ; —(CH 2 ) 0-4 OC(O)R ∘ ; —OC(O)(CH 2 ) 0-4 SR—, SC(S)SR ∘ ; —(CH 2 ) 0-4 SC(O)R ∘ ; —(CH 2 ) 0-4 C(O)NR ∘   2 ; —C(S)NR ∘   2 ; —C(S)SR ∘ ; —SC(S)SR ∘ , —(CH 2 ) 0-4 OC(O)NR ∘   2 ; —C(O)N(OR ∘ )R ∘ ; —C(O)C(O)R ∘ ; —C(O)CH 2 C(O)R ∘ ; —C(NOR ∘ )R ∘ ; —(CH 2 ) 0-4 SSR ∘ ; —(CH 2 ) 0-4 S(O) 2 R ∘ ; —(CH 2 ) 0-4 S(O) 2 OR ∘ ; —(CH 2 ) 0-4 OS(O) 2 R ∘ ; —S(O) 2 NR ∘   2 ; —(CH 2 ) 0-4 S(O)R ∘ ; —N(R ∘ )S(O) 2 NR ∘   2 ; —N(R ∘ )S(O) 2 R ∘ ; —N(OR ∘ )R ∘ ; —C(NH)NR ∘   2 ; —P(O) 2 R ∘ ; —P(O)R ∘   2 ; —OP(O)R ∘   2 ; —OP(O)(OR ∘ ) 2 ; —SiR ∘   3 ; —(C 1-4  straight or branched alkylene)O—N(R ∘ ) 2 ; or —(C 1-4  straight or branched alkylene)C(O)O—N(R ∘ ) 2 , wherein each R ∘  may be substituted as defined below and is independently hydrogen, C 1-6  aliphatic, —CH 2 Ph, —O(CH 2 ) 0-1 Ph, —CH 2 -(5-6 membered heteroaryl ring), or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R ∘ , taken together with their intervening atom(s), form a 3-12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, which may be substituted as defined below. 
     Suitable monovalent substituents on R ∘  (or the ring formed by taking two independent occurrences of R o  together with their intervening atoms), are independently halogen, —(CH 2 ) 0-2 R ● , -(haloR ● ), —(CH 2 ) 0-2 OH, —(CH 2 ) 0-2 OR ● , —(CH 2 ) 0-2 CH(OR ● ) 2 ; —O(haloR ● ), —CN, —N 3 , —(CH 2 ) 0-2 C(O)R ● , —(CH 2 ) 0-2 C(O)OH, —(CH 2 ) 0-2 C(O)OR ● , —(CH 2 ) 0-2 SR ● , —(CH 2 ) 0-2 SH, —(CH 2 ) 0-2 NH 2 , —(CH 2 ) 0-2 NHR ● , —(CH 2 ) 0-2 NR ●   2 , —NO 2 , —SiR ●   3 , —OSiR ●   3 , —C(O)SR ● , —(C 1-4  straight or branched alkylene)C(O)OR ● , or —SSR ●  wherein each R ●  is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently selected from C 1-4  aliphatic, —CH 2 Ph, —O(CH 2 ) 0-1 Ph, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. Suitable divalent substituents on a saturated carbon atom of R ∘  include ═O and ═S. 
     Suitable divalent substituents on a saturated carbon atom of an “optionally substituted” group include the following: ═O, ═S, ═NNR* 2 , ═NNHC(O)R*, ═NNHC(O)OR*, ═NNHS(O) 2 R*, ═NR*, ═NOR*, —O(C(R* 2 )) 2-3 O—, or —S(C(R* 2 )) 2-3 S—, wherein each independent occurrence of R* is selected from hydrogen, C 1-6  aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. Suitable divalent substituents that are bound to vicinal substitutable carbons of an “optionally substituted” group include: —O(CR* 2 ) 2-3 O—, wherein each independent occurrence of R* is selected from hydrogen, C 1-6  aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     Suitable substituents on the aliphatic group of R* include halogen, —R ● , -(haloR ● ), —OH, —OR ● , —O(haloR ● ), —CN, —C(O)OH, —C(O)OR ● , —NH 2 , —NHR ● , —NR ●   2 , or —NO 2 , wherein each R ●  is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently C 1-4  aliphatic, —CH 2 Ph, —O(CH 2 ) 0-1 Ph, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     Suitable substituents on a substitutable nitrogen of an “optionally substituted” group include —R † , —NR †   2 , —C(O)R † , —C(O)OR † , —C(O)C(O)R † , —C(O)CH 2 C(O)R † , —S(O) 2 R † , —S(O) 2 NR †   2 , —C(S)NR †   2 , —C(NH)NR †   2 , or —N(R † )S(O) 2 R † ; wherein each R †  is independently hydrogen, C 1-6  aliphatic which may be substituted as defined below, unsubstituted —OPh, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R † , taken together with their intervening atom(s) form an unsubstituted 3-12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     Suitable substituents on the aliphatic group of R †  are independently halogen, —R ● , -(haloR ● ), —OH, —OR ● , —O(haloR ● ), —CN, —C(O)OH, —C(O)OR ● , —NH 2 , —NHR ● , —NR ●   2 , or —NO 2 , wherein each R ●  is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently C 1-4  aliphatic, —CH 2 Ph, —O(CH 2 ) 0-1 Ph, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     Oral: The phrases “oral administration” and “administered orally” as used herein have their art-understood meaning referring to administration by mouth of a compound or composition. 
     Parenteral: The phrases “parenteral administration” and “administered parenterally” as used herein have their art-understood meaning referring to modes of administration other than enteral and topical administration, usually by injection, and include, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticulare, subcapsular, subarachnoid, intraspinal, and intrasternal injection and infusion. 
     Partially unsaturated: As used herein, the term “partially unsaturated” refers to a ring moiety that includes at least one double or triple bond. The term “partially unsaturated” is intended to encompass rings having multiple sites of unsaturation, but is not intended to include aryl or heteroaryl moieties, as herein defined. 
     Pharmaceutical composition: As used herein, the term “pharmaceutical composition” refers to an active agent, formulated together with one or more pharmaceutically acceptable carriers. In some embodiments, active agent is present in unit dose amount appropriate for administration in a therapeutic regimen that shows a statistically significant probability of achieving a predetermined therapeutic effect when administered to a relevant population. In some embodiments, pharmaceutical compositions may be specially formulated for administration in solid or liquid form, including those adapted for the following: oral administration, for example, drenches (aqueous or non-aqueous solutions or suspensions), tablets, e.g., those targeted for buccal, sublingual, and systemic absorption, boluses, powders, granules, pastes for application to the tongue; parenteral administration, for example, by subcutaneous, intramuscular, intravenous or epidural injection as, for example, a sterile solution or suspension, or sustained-release formulation; topical application, for example, as a cream, ointment, or a controlled-release patch or spray applied to the skin, lungs, or oral cavity; intravaginally or intrarectally, for example, as a pessary, cream, or foam; sublingually; ocularly; transdermally; or nasally, pulmonary, and to other mucosal surfaces. 
     Pharmaceutically acceptable: As used herein, the phrase “pharmaceutically acceptable” refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio. 
     Pharmaceutically acceptable carrier: As used herein, the term “pharmaceutically acceptable carrier” means a pharmaceutically-acceptable material, composition or vehicle, such as a liquid or solid filler, diluent, excipient, or solvent encapsulating material, involved in carrying or transporting the subject compound from one organ, or portion of the body, to another organ, or portion of the body. Each carrier must be “acceptable” in the sense of being compatible with the other ingredients of the formulation and not injurious to the patient. Some examples of materials which can serve as pharmaceutically-acceptable carriers include: sugars, such as lactose, glucose and sucrose; starches, such as corn starch and potato starch; cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients, such as cocoa butter and suppository waxes; oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols, such as propylene glycol; polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; esters, such as ethyl oleate and ethyl laurate; agar; buffering agents, such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer&#39;s solution; ethyl alcohol; pH buffered solutions; polyesters, polycarbonates and/or polyanhydrides; and other non-toxic compatible substances employed in pharmaceutical formulations. 
     Pharmaceutically acceptable salt: The term “pharmaceutically acceptable salt”, as used herein, refers to salts of such compounds that are appropriate for use in pharmaceutical contexts, i.e., salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge, et al. describes pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 66: 1-19 (1977). In some embodiments, pharmaceutically acceptable salt include, but are not limited to, nontoxic acid addition salts, which are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. In some embodiments, pharmaceutically acceptable salts include, but are not limited to, adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. In some embodiments, pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, alkyl having from 1 to 6 carbon atoms, sulfonate and aryl sulfonate. 
     Prodrug: A general, a “prodrug,” as that term is used herein and as is understood in the art, is an entity that, when administered to an organism, is metabolized in the body to deliver an active (e.g., therapeutic or diagnostic) agent of interest. Typically, such metabolism involves removal of at least one “prodrug moiety” so that the active agent is formed. Various forms of “prodrugs” are known in the art. For examples of such prodrug moieties, see:
         a)  Design of Prodrugs , edited by H. Bundgaard, (Elsevier, 1985) and  Methods in Enzymology,  42:309-396, edited by K. Widder, et al. (Academic Press, 1985);   b)  Prodrugs and Targeted Delivery , edited by by J. Rautio (Wiley, 2011);   c)  Prodrugs and Targeted Delivery , edited by by J. Rautio (Wiley, 2011);   d) A Textbook of Drug Design and Development, edited by Krogsgaard-Larsen;   e) Bundgaard, Chapter 5 “Design and Application of Prodrugs”, by H. Bundgaard, p. 113-191 (1991);   f) Bundgaard,  Advanced Drug Delivery Reviews,  8:1-38 (1992);   g) Bundgaard, et al.,  Journal of Pharmaceutical Sciences,  77:285 (1988); and   h) Kakeya, et al.,  Chem. Pharm. Bull.,  32:692 (1984).       

     As with other compounds described herein, prodrugs may be provided in any of a variety of forms, e.g., crystal forms, salt forms etc. In some embodiments, prodrugs are provided as pharmaceutically acceptable salts thereof. 
     Protecting group: The term “protecting group,” as used herein, is well known in the art and includes those described in detail in  Protecting Groups in Organic Synthesis , T. W. Greene and P. G. M. Wuts, 3 rd  edition, John Wiley &amp; Sons, 1999, the entirety of which is incorporated herein by reference. Also included are those protecting groups specially adapted for nucleoside and nucleotide chemistry described in  Current Protocols in Nucleic Acid Chemistry , edited by Serge L. Beaucage et al. June 2012, the entirety of Chapter 2 is incorporated herein by reference. Suitable amino-protecting groups include methyl carbamate, ethyl carbamate, 9-fluorenylmethyl carbamate (Fmoc), 9-(2-sulfo)fluorenylmethyl carbamate, 9-(2,7-dibromo)fluoroenylmethyl carbamate, 2,7-di-t-butyl-[9-(10,10-dioxo-10,10,10,10-tetrahydrothioxanthyl)]methyl carbamate (DBD-Tmoc), 4-methoxyphenacyl carbamate (Phenoc), 2,2,2-trichloroethyl carbamate (Troc), 2-trimethylsilylethyl carbamate (Teoc), 2-phenylethyl carbamate (hZ), 1-(1-adamantyl)-1-methylethyl carbamate (Adpoc), 1,1-dimethyl-2-haloethyl carbamate, 1,1-dimethyl-2,2-dibromoethyl carbamate (DB-t-BOC), 1,1-dimethyl-2,2,2-trichloroethyl carbamate (TCBOC), 1-methyl-1-(4-biphenylyl)ethyl carbamate (Bpoc), 1-(3,5-di-t-butylphenyl)-1-methylethyl carbamate (t-Bumeoc), 2-(2′- and 4′-pyridyl)ethyl carbamate (Pyoc), 2-(N,N-dicyclohexylcarboxamido)ethyl carbamate, t-butyl carbamate (BOC), 1-adamantyl carbamate (Adoc), vinyl carbamate (Voc), allyl carbamate (Alloc), 1-isopropylallyl carbamate (Ipaoc), cinnamyl carbamate (Coc), 4-nitrocinnamyl carbamate (Noc), 8-quinolyl carbamate, N-hydroxypiperidinyl carbamate, alkyldithio carbamate, benzyl carbamate (Cbz), p-methoxybenzyl carbamate (Moz), p-nitrobenzyl carbamate, p-bromobenzyl carbamate, p-chlorobenzyl carbamate, 2,4-dichlorobenzyl carbamate, 4-methylsulfinylbenzyl carbamate (Msz), 9-anthrylmethyl carbamate, diphenylmethyl carbamate, 2-methylthioethyl carbamate, 2-methylsulfonylethyl carbamate, 2-(p-toluenesulfonyl)ethyl carbamate, [2-(1,3-dithianyl)]methyl carbamate (Dmoc), 4-methylthiophenyl carbamate (Mtpc), 2,4-dimethylthiophenyl carbamate (Bmpc), 2-phosphonioethyl carbamate (Peoc), 2-triphenylphosphonioisopropyl carbamate (Ppoc), 1,1-dimethyl-2-cyanoethyl carbamate, m-chloro-p-acyloxybenzyl carbamate, p-(dihydroxyboryl)benzyl carbamate, 5-benzisoxazolylmethyl carbamate, 2-(trifluoromethyl)-6-chromonylmethyl carbamate (Tcroc), m-nitrophenyl carbamate, 3,5-dimethoxybenzyl carbamate, o-nitrobenzyl carbamate, 3,4-dimethoxy-6-nitrobenzyl carbamate, phenyl(o-nitrophenyl)methyl carbamate, phenothiazinyl-(10)-carbonyl derivative, N′-p-toluenesulfonylaminocarbonyl derivative, N′-phenylaminothiocarbonyl derivative, t-amyl carbamate, S-benzyl thiocarbamate, p-cyanobenzyl carbamate, cyclobutyl carbamate, cyclohexyl carbamate, cyclopentyl carbamate, cyclopropylmethyl carbamate, p-decyloxybenzyl carbamate, 2,2-dimethoxycarbonylvinyl carbamate, o-(N,N-dimethylcarboxamido)benzyl carbamate, 1,1-dimethyl-3-(N,N-dimethylcarboxamido)propyl carbamate, 1,1-dimethylpropynyl carbamate, di(2-pyridyl)methyl carbamate, 2-furanylmethyl carbamate, 2-iodoethyl carbamate, isoborynl carbamate, isobutyl carbamate, isonicotinyl carbamate, p-(p′-methoxyphenylazo)benzyl carbamate, 1-methylcyclobutyl carbamate, 1-methylcyclohexyl carbamate, 1-methyl-1-cyclopropylmethyl carbamate, 1-methyl-1-(3,5-dimethoxyphenyl)ethyl carbamate, 1-methyl-1-(p-phenylazophenyl)ethyl carbamate, 1-methyl-1-phenylethyl carbamate, 1-methyl-1-(4-pyridyl)ethyl carbamate, phenyl carbamate, p-(phenylazo)benzyl carbamate, 2,4,6-tri-t-butylphenyl carbamate, 4-(trimethylammonium)benzyl carbamate, 2,4,6-trimethylbenzyl carbamate, formamide, acetamide, chloroacetamide, trichloroacetamide, trifluoroacetamide, phenylacetamide, 3-phenylpropanamide, picolinamide, 3-pyridylcarboxamide, N-benzoylphenylalanyl derivative, benzamide, p-phenylbenzamide, o-nitophenylacetamide, o-nitrophenoxyacetamide, acetoacetamide, (N′-dithiobenzyloxycarbonylamino)acetamide, 3-(p-hydroxyphenyl)propanamide, 3-(o-nitrophenyl)propanamide, 2-methyl-2-(o-nitrophenoxy)propanamide, 2-methyl-2-(o-phenylazophenoxy)propanamide, 4-chlorobutanamide, 3-methyl-3-nitrobutanamide, o-nitrocinnamide, N-acetylmethionine derivative, o-nitrobenzamide, o-(benzoyloxymethyl)benzamide, 4,5-diphenyl-3-oxazolin-2-one, N-phthalimide, N-dithiasuccinimide (Dts), N-2,3-diphenylmaleimide, N-2,5-dimethylpyrrole, N-1,1,4,4-tetramethyldisilylazacyclopentane adduct (STABASE), 5-substituted 1,3-dimethyl-1,3,5-triazacyclohexan-2-one, 5-substituted 1,3-dibenzyl-1,3,5-triazacyclohexan-2-one, 1-substituted 3,5-dinitro-4-pyridone, N-methylamine, N-allylamine, N-[2-(trimethylsilyl)ethoxy]methylamine (SEM), N-3-acetoxypropylamine, N-(1-isopropyl-4-nitro-2-oxo-3-pyroolin-3-yl)amine, quaternary ammonium salts, N-benzylamine, N-di(4-methoxyphenyl)methylamine, N-5-dibenzosuberylamine, N-triphenylmethylamine (Tr), N-[(4-methoxyphenyl)diphenylmethyl]amine (MMTr), N-9-phenylfluorenylamine (PhF), N-2,7-dichloro-9-fluorenylmethyleneamine, N-ferrocenylmethylamino (Fcm), N-2-picolylamino N′-oxide, N-1,1-dimethylthiomethyleneamine, N-benzylideneamine, N-p-methoxybenzylideneamine, N-diphenylmethyleneamine, N-[(2-pyridyl)mesityl]methyleneamine, N—(N′,N′-dimethylaminomethylene)amine, N,N′-isopropylidenediamine, N-p-nitrobenzylideneamine, N-salicylideneamine, N-5-chlorosalicylideneamine, N-(5-chloro-2-hydroxyphenyl)phenylmethyleneamine, N-cyclohexylideneamine, N-(5,5-dimethyl-3-oxo-1-cyclohexenyl)amine, N-borane derivative, N-diphenylborinic acid derivative, N-[phenyl(pentacarbonylchromium- or tungsten)carbonyl]amine, N-copper chelate, N-zinc chelate, N-nitroamine, N-nitrosoamine, amine N-oxide, diphenylphosphinamide (Dpp), dimethylthiophosphinamide (Mpt), diphenylthiophosphinamide (Ppt), dialkyl phosphoramidates, dibenzyl phosphoramidate, diphenyl phosphoramidate, benzenesulfenamide, o-nitrobenzenesulfenamide (Nps), 2,4-dinitrobenzenesulfenamide, pentachlorobenzenesulfenamide, 2-nitro-4-methoxybenzenesulfenamide, triphenylmethylsulfenamide, 3-nitropyridinesulfenamide (Npys), p-toluenesulfonamide (Ts), benzenesulfonamide, 2,3,6-trimethyl-4-methoxybenzenesulfonamide (Mtr), 2,4,6-trimethoxybenzenesulfonamide (Mtb), 2,6-dimethyl-4-methoxybenzenesulfonamide (Pme), 2,3,5,6-tetramethyl-4-methoxybenzenesulfonamide (Mte), 4-methoxybenzenesulfonamide (Mbs), 2,4,6-trimethylbenzenesulfonamide (Mts), 2,6-dimethoxy-4-methylbenzenesulfonamide (iMds), 2,2,5,7,8-pentamethylchroman-6-sulfonamide (Pmc), methanesulfonamide (Ms), β-trimethylsilylethanesulfonamide (SES), 9-anthracenesulfonamide, 4-(4′,8′-dimethoxynaphthylmethyl)benzenesulfonamide (DNMBS), benzylsulfonamide, trifluoromethylsulfonamide, and phenacylsulfonamide. 
     Suitably protected carboxylic acids further include, but are not limited to, silyl-, alkyl-, alkenyl-, aryl-, and arylalkyl-protected carboxylic acids. Examples of suitable silyl groups include trimethylsilyl, triethylsilyl, t-butyldimethylsilyl, t-butyldiphenylsilyl, triisopropylsilyl, and the like. Examples of suitable alkyl groups include methyl, benzyl, p-methoxybenzyl, 3,4-dimethoxybenzyl, trityl, t-butyl, tetrahydropyran-2-yl. Examples of suitable alkenyl groups include allyl. Examples of suitable aryl groups include optionally substituted phenyl, biphenyl, or naphthyl. Examples of suitable arylalkyl groups include optionally substituted benzyl (e.g., p-methoxybenzyl (MPM), 3,4-dimethoxybenzyl, O-nitrobenzyl, p-nitrobenzyl, p-halobenzyl, 2,6-dichlorobenzyl, p-cyanobenzyl), and 2- and 4-picolyl. 
     Suitable hydroxyl protecting groups include methyl, methoxylmethyl (MOM), methylthiomethyl (MTM), t-butylthiomethyl, (phenyldimethylsilyl)methoxymethyl (SMOM), benzyloxymethyl (BOM), p-methoxybenzyloxymethyl (PMBM), (4-methoxyphenoxy)methyl (p-AOM), guaiacolmethyl (GUM), t-butoxymethyl, 4-pentenyloxymethyl (POM), siloxymethyl, 2-methoxyethoxymethyl (MEM), 2,2,2-trichloroethoxymethyl, bis(2-chloroethoxy)methyl, 2-(trimethylsilyl)ethoxymethyl (SEMOR), tetrahydropyranyl (THP), 3-bromotetrahydropyranyl, tetrahydrothiopyranyl, 1-methoxycyclohexyl, 4-methoxytetrahydropyranyl (MTHP), 4-methoxytetrahydrothiopyranyl, 4-methoxytetrahydrothiopyranyl S,S-dioxide, 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (CTMP), 1,4-dioxan-2-yl, tetrahydrofuranyl, tetrahydrothiofuranyl, 2,3,3a,4,5,6,7,7a-octahydro-7,8,8-trimethyl-4,7-methanobenzofuran-2-yl, 1-ethoxyethyl, 1-(2-chloroethoxy)ethyl, 1-methyl-1-methoxyethyl, 1-methyl-1-benzyloxyethyl, 1-methyl-1-benzyloxy-2-fluoroethyl, 2,2,2-trichloroethyl, 2-trimethylsilylethyl, 2-(phenylselenyl)ethyl, t-butyl, allyl, p-chlorophenyl, p-methoxyphenyl, 2,4-dinitrophenyl, benzyl, p-methoxybenzyl, 3,4-dimethoxybenzyl, o-nitrobenzyl, p-nitrobenzyl, p-halobenzyl, 2,6-dichlorobenzyl, p-cyanobenzyl, p-phenylbenzyl, 2-picolyl, 4-picolyl, 3-methyl-2-picolyl N-oxido, diphenylmethyl, p,p′-dinitrobenzhydryl, 5-dibenzosuberyl, triphenylmethyl, α-naphthyldiphenylmethyl, p-methoxyphenyldiphenylmethyl, di(p-methoxyphenyl)phenylmethyl, tri(p-methoxyphenyl)methyl, 4-(4′-bromophenacyloxyphenyl)diphenylmethyl, 4,4′,4″-tris(4,5-dichlorophthalimidophenyl)methyl, 4,4′,4″-tris(levulinoyloxyphenyl)methyl, 4,4′,4″-tris(benzoyloxyphenyl)methyl, 3-(imidazol-1-yl)bis(4′,4″-dimethoxyphenyl)methyl, 1,1-bis(4-methoxyphenyl)-1′-pyrenylmethyl, 9-anthryl, 9-(9-phenyl)xanthenyl, 9-(9-phenyl-10-oxo)anthryl, 1,3-benzodithiolan-2-yl, benzisothiazolyl S,S-dioxido, trimethylsilyl (TMS), triethylsilyl (TES), triisopropylsilyl (TIPS), dimethylisopropylsilyl (IPDMS), diethylisopropylsilyl (DEIPS), dimethylthexylsilyl, t-butyldimethylsilyl (TBDMS), t-butyldiphenylsilyl (TBDPS), tribenzylsilyl, tri-p-xylylsilyl, triphenylsilyl, diphenylmethylsilyl (DPMS), t-butylmethoxyphenylsilyl (TBMPS), formate, benzoylformate, acetate, chloroacetate, dichloroacetate, trichloroacetate, trifluoroacetate, methoxyacetate, triphenylmethoxyacetate, phenoxyacetate, p-chlorophenoxyacetate, 3-phenylpropionate, 4-oxopentanoate (levulinate), 4,4-(ethylenedithio)pentanoate (levulinoyldithioacetal), pivaloate, adamantoate, crotonate, 4-methoxycrotonate, benzoate, p-phenylbenzoate, 2,4,6-trimethylbenzoate (mesitoate), alkyl methyl carbonate, 9-fluorenylmethyl carbonate (Fmoc), alkyl ethyl carbonate, alkyl 2,2,2-trichloroethyl carbonate (Troc), 2-(trimethylsilyl)ethyl carbonate (TMSEC), 2-(phenylsulfonyl) ethyl carbonate (Psec), 2-(triphenylphosphonio) ethyl carbonate (Peoc), alkyl isobutyl carbonate, alkyl vinyl carbonate alkyl allyl carbonate, alkyl p-nitrophenyl carbonate, alkyl benzyl carbonate, alkyl p-methoxybenzyl carbonate, alkyl 3,4-dimethoxybenzyl carbonate, alkyl o-nitrobenzyl carbonate, alkyl p-nitrobenzyl carbonate, alkyl S-benzyl thiocarbonate, 4-ethoxy-1-naphthyl carbonate, methyl dithiocarbonate, 2-iodobenzoate, 4-azidobutyrate, 4-nitro-4-methylpentanoate, o-(dibromomethyl)benzoate, 2-formylbenzenesulfonate, 2-(methylthiomethoxy)ethyl, 4-(methylthiomethoxy)butyrate, 2-(methylthiomethoxymethyl)benzoate, 2,6-dichloro-4-methylphenoxyacetate, 2,6-dichloro-4-(1,1,3,3-tetramethylbutyl)phenoxyacetate, 2,4-bis(1,1-dimethylpropyl)phenoxyacetate, chlorodiphenylacetate, isobutyrate, monosuccinate, (E)-2-methyl-2-butenoate, o-(methoxycarbonyl)benzoate, α-naphthoate, nitrate, alkyl N,N,N′,N′-tetramethylphosphorodiamidate, alkyl N-phenylcarbamate, borate, dimethylphosphinothioyl, alkyl 2,4-dinitrophenylsulfenate, sulfate, methanesulfonate (mesylate), benzylsulfonate, and tosylate (Ts). For protecting 1,2- or 1,3-diols, the protecting groups include methylene acetal, ethylidene acetal, 1-t-butylethylidene ketal, 1-phenylethylidene ketal, (4-methoxyphenyl)ethylidene acetal, 2,2,2-trichloroethylidene acetal, acetonide, cyclopentylidene ketal, cyclohexylidene ketal, cycloheptylidene ketal, benzylidene acetal, p-methoxybenzylidene acetal, 2,4-dimethoxybenzylidene ketal, 3,4-dimethoxybenzylidene acetal, 2-nitrobenzylidene acetal, methoxymethylene acetal, ethoxymethylene acetal, dimethoxymethylene ortho ester, 1-methoxyethylidene ortho ester, 1-ethoxyethylidine ortho ester, 1,2-dimethoxyethylidene ortho ester, α-methoxybenzylidene ortho ester, 1-(N,N-dimethylamino)ethylidene derivative, α-(N,N′-dimethylamino)benzylidene derivative, 2-oxacyclopentylidene ortho ester, di-t-butylsilylene group (DTBS), 1,3-(1,1,3,3-tetraisopropyldisiloxanylidene) derivative (TIPDS), tetra-t-butoxydisiloxane-1,3-diylidene derivative (TBDS), cyclic carbonates, cyclic boronates, ethyl boronate, and phenyl boronate. 
     In some embodiments, a hydroxyl protecting group is acetyl, t-butyl, t-butoxymethyl, methoxymethyl, tetrahydropyranyl, 1-ethoxyethyl, 1-(2-chloroethoxy)ethyl, 2-trimethylsilylethyl, p-chlorophenyl, 2,4-dinitrophenyl, benzyl, benzoyl, p-phenylbenzoyl, 2,6-dichlorobenzyl, diphenylmethyl, p-nitrobenzyl, triphenylmethyl (trityl), 4,4′-dimethoxytrityl, trimethylsilyl, triethylsilyl, t-butyldimethylsilyl, t-butyldiphenylsilyl, triphenylsilyl, triisopropylsilyl, benzoylformate, chloroacetyl, trichloroacetyl, trifluoroacetyl, pivaloyl, 9-fluorenylmethyl carbonate, mesylate, tosylate, triflate, trityl, monomethoxytrityl (MMTr), 4,4′-dimethoxytrityl, (DMTr) and 4,4′,4″-trimethoxytrityl (TMTr), 2-cyanoethyl (CE or Cne), 2-(trimethylsilyl)ethyl (TSE), 2-(2-nitrophenyl)ethyl, 2-(4-cyanophenyl)ethyl 2-(4-nitrophenyl)ethyl (NPE), 2-(4-nitrophenylsulfonyl)ethyl, 3,5-dichlorophenyl, 2,4-dimethylphenyl, 2-nitrophenyl, 4-nitrophenyl, 2,4,6-trimethylphenyl, 2-(2-nitrophenyl)ethyl, butylthiocarbonyl, 4,4′,4″-tris(benzoyloxy)trityl, diphenylcarbamoyl, levulinyl, 2-(dibromomethyl)benzoyl (Dbmb), 2-(isopropylthiomethoxymethyl)benzoyl (Ptmt), 9-phenylxanthen-9-yl (pixyl) or 9-(p-methoxyphenyl)xanthine-9-yl (MOX). In some embodiments, each of the hydroxyl protecting groups is, independently selected from acetyl, benzyl, t-butyldimethylsilyl, t-butyldiphenylsilyl and 4,4′-dimethoxytrityl. In some embodiments, the hydroxyl protecting group is selected from the group consisting of trityl, monomethoxytrityl and 4,4′-dimethoxytrityl group. 
     In some embodiments, a phosphorous protecting group is a group attached to the internucleotide phosphorous linkage throughout oligonucleotide synthesis. In some embodiments, the phosphorous protecting group is attached to the sulfur atom of the internucleotide phosphorothioate linkage. In some embodiments, the phosphorous protecting group is attached to the oxygen atom of the internucleotide phosphorothioate linkage. In some embodiments, the phosphorous protecting group is attached to the oxygen atom of the internucleotide phosphate linkage. In some embodiments the phosphorous protecting group is 2-cyanoethyl (CE or Cne), 2-trimethylsilylethyl, 2-nitroethyl, 2-sulfonylethyl, methyl, benzyl, o-nitrobenzyl, 2-(p-nitrophenyl)ethyl (NPE or Npe), 2-phenylethyl, 3-(N-tert-butylcarboxamido)-1-propyl, 4-oxopentyl, 4-methylthio-1-butyl, 2-cyano-1,1-dimethylethyl, 4-N-methylaminobutyl, 3-(2-pyridyl)-1-propyl, 2-[N-methyl-N-(2-pyridyl)]aminoethyl, 2-(N-formyl,N-methyl)aminoethyl, 4-[N-methyl-N-(2,2,2-trifluoroacetyl)amino]butyl. 
     Protein: As used herein, the term “protein” refers to a polypeptide (i.e., a string of at least two amino acids linked to one another by peptide bonds). In some embodiments, proteins include only naturally-occurring amino acids. In some embodiments, proteins include one or more non-naturally-occurring amino acids (e.g., moieties that form one or more peptide bonds with adjacent amino acids). In some embodiments, one or more residues in a protein chain contain a non-amino-acid moiety (e.g., a glycan, etc). In some embodiments, a protein includes more than one polypeptide chain, for example linked by one or more disulfide bonds or associated by other means. In some embodiments, proteins contain L-amino acids, D-amino acids, or both; in some embodiments, proteins contain one or more amino acid modifications or analogs known in the art. Useful modifications include, e.g., terminal acetylation, amidation, methylation, etc. The term “peptide” is generally used to refer to a polypeptide having a length of less than about 100 amino acids, less than about 50 amino acids, less than 20 amino acids, or less than 10 amino acids. In some embodiments, proteins are antibodies, antibody fragments, biologically active portions thereof, and/or characteristic portions thereof. 
     Sample: As used herein, the term “sample” refers to a biological sample obtained or derived from a source of interest, as described herein. In some embodiments, a source of interest comprises an organism, such as an animal or human. In some embodiments, a biological sample comprises biological tissue or fluid. In some embodiments, a biological sample is or comprises bone marrow; blood; blood cells; ascites; tissue or fine needle biopsy samples; cell-containing body fluids; free floating nucleic acids; sputum; saliva; urine; cerebrospinal fluid, peritoneal fluid; pleural fluid; feces; lymph; gynecological fluids; skin swabs; vaginal swabs; oral swabs; nasal swabs; washings or lavages such as a ductal lavages or bronchioalveolar lavages; aspirates; scrapings; bone marrow specimens; tissue biopsy specimens; surgical specimens; feces, other body fluids, secretions, and/or excretions; and/or cells therefrom, etc. In some embodiments, a biological sample is or comprises cells obtained from an individual. In some embodiments, a sample is a “primary sample” obtained directly from a source of interest by any appropriate means. For example, in some embodiments, a primary biological sample is obtained by methods selected from the group consisting of biopsy (e.g., fine needle aspiration or tissue biopsy), surgery, collection of body fluid (e.g., blood, lymph, feces etc.), etc. In some embodiments, as will be clear from context, the term “sample” refers to a preparation that is obtained by processing (e.g., by removing one or more components of and/or by adding one or more agents to) a primary sample. For example, filtering using a semi-permeable membrane. Such a “processed sample” may comprise, for example nucleic acids or proteins extracted from a sample or obtained by subjecting a primary sample to techniques such as amplification or reverse transcription of mRNA, isolation and/or purification of certain components, etc. 
     Stereochemically isomeric forms: The phrase “stereochemically isomeric forms,” as used herein, refers to different compounds made up of the same atoms bonded by the same sequence of bonds but having different three-dimensional structures which are not interchangeable. In some embodiments of the invention, provided chemical compositions may be or include pure preparations of individual stereochemically isomeric forms of a compound; in some embodiments, provided chemical compositions may be or include mixtures of two or more stereochemically isomeric forms of the compound. In certain embodiments, such mixtures contain equal amounts of different stereochemically isomeric forms; in certain embodiments, such mixtures contain different amounts of at least two different stereochemically isomeric forms. In some embodiments, a chemical composition may contain all diastereomers and/or enantiomers of the compound. In some embodiments, a chemical composition may contain less than all diastereomers and/or enantiomers of a compound. In some embodiments, if a particular enantiomer of a compound of the present invention is desired, it may be prepared, for example, by asymmetric synthesis, or by derivation with a chiral auxiliary, where the resulting diastereomeric mixture is separated and the auxiliary group cleaved to provide the pure desired enantiomers. Alternatively, where the molecule contains a basic functional group, such as amino, diastereomeric salts are formed with an appropriate optically-active acid, and resolved, for example, by fractional crystallization. 
     Subject: As used herein, the term “subject” or “test subject” refers to any organism to which a provided compound or composition is administered in accordance with the present invention e.g., for experimental, diagnostic, prophylactic, and/or therapeutic purposes. Typical subjects include animals (e.g., mammals such as mice, rats, rabbits, non-human primates, and humans; insects; worms; etc.) and plants. In some embodiments, a subject may be suffering from, and/or susceptible to a disease, disorder, and/or condition. 
     Substantially: As used herein, the term “substantially” refers to the qualitative condition of exhibiting total or near-total extent or degree of a characteristic or property of interest. One of ordinary skill in the biological arts will understand that biological and chemical phenomena rarely, if ever, go to completion and/or proceed to completeness or achieve or avoid an absolute result. The term “substantially” is therefore used herein to capture the potential lack of completeness inherent in many biological and/or chemical phenomena. 
     Suffering from: An individual who is “suffering from” a disease, disorder, and/or condition has been diagnosed with and/or displays one or more symptoms of a disease, disorder, and/or condition. 
     Susceptible to: An individual who is “susceptible to” a disease, disorder, and/or condition is one who has a higher risk of developing the disease, disorder, and/or condition than does a member of the general public. In some embodiments, an individual who is susceptible to a disease, disorder and/or condition may not have been diagnosed with the disease, disorder, and/or condition. In some embodiments, an individual who is susceptible to a disease, disorder, and/or condition may exhibit symptoms of the disease, disorder, and/or condition. In some embodiments, an individual who is susceptible to a disease, disorder, and/or condition may not exhibit symptoms of the disease, disorder, and/or condition. In some embodiments, an individual who is susceptible to a disease, disorder, and/or condition will develop the disease, disorder, and/or condition. In some embodiments, an individual who is susceptible to a disease, disorder, and/or condition will not develop the disease, disorder, and/or condition. 
     Systemic: The phrases “systemic administration,” “administered systemically,” “peripheral administration,” and “administered peripherally” as used herein have their art-understood meaning referring to administration of a compound or composition such that it enters the recipient&#39;s system. 
     Tautomeric forms: The phrase “tautomeric forms,” as used herein, is used to describe different isomeric forms of organic compounds that are capable of facile interconversion. Tautomers may be characterized by the formal migration of a hydrogen atom or proton, accompanied by a switch of a single bond and adjacent double bond. In some embodiments, tautomers may result from prototropic tautomerism (i.e., the relocation of a proton). In some embodiments, tautomers may result from valence tautomerism (i.e., the rapid reorganization of bonding electrons). All such tautomeric forms are intended to be included within the scope of the present invention. In some embodiments, tautomeric forms of a compound exist in mobile equilibrium with each other, so that attempts to prepare the separate substances results in the formation of a mixture. In some embodiments, tautomeric forms of a compound are separable and isolatable compounds. In some embodiments of the invention, chemical compositions may be provided that are or include pure preparations of a single tautomeric form of a compound. In some embodiments of the invention, chemical compositions may be provided as mixtures of two or more tautomeric forms of a compound. In certain embodiments, such mixtures contain equal amounts of different tautomeric forms; in certain embodiments, such mixtures contain different amounts of at least two different tautomeric forms of a compound. In some embodiments of the invention, chemical compositions may contain all tautomeric forms of a compound. In some embodiments of the invention, chemical compositions may contain less than all tautomeric forms of a compound. In some embodiments of the invention, chemical compositions may contain one or more tautomeric forms of a compound in amounts that vary over time as a result of interconversion. In some embodiments of the invention, the tautomerism is keto-enol tautomerism. One of skill in the chemical arts would recognize that a keto-enol tautomer can be “trapped” (i.e., chemically modified such that it remains in the “enol” form) using any suitable reagent known in the chemical arts in to provide an enol derivative that may subsequently be isolated using one or more suitable techniques known in the art. Unless otherwise indicated, the present invention encompasses all tautomeric forms of relevant compounds, whether in pure form or in admixture with one another. 
     Therapeutic agent: As used herein, the phrase “therapeutic agent” refers to any agent that, when administered to a subject, has a therapeutic effect and/or elicits a desired biological and/or pharmacological effect. In some embodiments, a therapeutic agent is any substance that can be used to alleviate, ameliorate, relieve, inhibit, prevent, delay onset of, reduce severity of, and/or reduce incidence of one or more symptoms or features of a disease, disorder, and/or condition. 
     Therapeutically effective amount: As used herein, the term “therapeutically effective amount” means an amount of a substance (e.g., a therapeutic agent, composition, and/or formulation) that elicits a desired biological response when administered as part of a therapeutic regimen. In some embodiments, a therapeutically effective amount of a substance is an amount that is sufficient, when administered to a subject suffering from or susceptible to a disease, disorder, and/or condition, to treat, diagnose, prevent, and/or delay the onset of the disease, disorder, and/or condition. As will be appreciated by those of ordinary skill in this art, the effective amount of a substance may vary depending on such factors as the desired biological endpoint, the substance to be delivered, the target cell or tissue, etc. For example, the effective amount of compound in a formulation to treat a disease, disorder, and/or condition is the amount that alleviates, ameliorates, relieves, inhibits, prevents, delays onset of, reduces severity of and/or reduces incidence of one or more symptoms or features of the disease, disorder, and/or condition. In some embodiments, a therapeutically effective amount is administered in a single dose; in some embodiments, multiple unit doses are required to deliver a therapeutically effective amount. 
     Treat: As used herein, the term “treat,” “treatment,” or “treating” refers to any method used to partially or completely alleviate, ameliorate, relieve, inhibit, prevent, delay onset of, reduce severity of, and/or reduce incidence of one or more symptoms or features of a disease, disorder, and/or condition. Treatment may be administered to a subject who does not exhibit signs of a disease, disorder, and/or condition. In some embodiments, treatment may be administered to a subject who exhibits only early signs of the disease, disorder, and/or condition, for example for the purpose of decreasing the risk of developing pathology associated with the disease, disorder, and/or condition. 
     Unsaturated: The term “unsaturated,” as used herein, means that a moiety has one or more units of unsaturation. 
     Unit dose: The expression “unit dose” as used herein refers to an amount administered as a single dose and/or in a physically discrete unit of a pharmaceutical composition. In many embodiments, a unit dose contains a predetermined quantity of an active agent. In some embodiments, a unit dose contains an entire single dose of the agent. In some embodiments, more than one unit dose is administered to achieve a total single dose. In some embodiments, administration of multiple unit doses is required, or expected to be required, in order to achieve an intended effect. A unit dose may be, for example, a volume of liquid (e.g., an acceptable carrier) containing a predetermined quantity of one or more therapeutic agents, a predetermined amount of one or more therapeutic agents in solid form, a sustained release formulation or drug delivery device containing a predetermined amount of one or more therapeutic agents, etc. It will be appreciated that a unit dose may be present in a formulation that includes any of a variety of components in addition to the therapeutic agent(s). For example, acceptable carriers (e.g., pharmaceutically acceptable carriers), diluents, stabilizers, buffers, preservatives, etc., may be included as described infra. It will be appreciated by those skilled in the art, in many embodiments, a total appropriate daily dosage of a particular therapeutic agent may comprise a portion, or a plurality, of unit doses, and may be decided, for example, by the attending physician within the scope of sound medical judgment. In some embodiments, the specific effective dose level for any particular subject or organism may depend upon a variety of factors including the disorder being treated and the severity of the disorder; activity of specific active compound employed; specific composition employed; age, body weight, general health, sex and diet of the subject; time of administration, and rate of excretion of the specific active compound employed; duration of the treatment; drugs and/or additional therapies used in combination or coincidental with specific compound(s) employed, and like factors well known in the medical arts. 
     Wild-type: As used herein, the term “wild-type” has its art-understood meaning that refers to an entity having a structure and/or activity as found in nature in a “normal” (as contrasted with mutant, diseased, altered, etc) state or context. Those of ordinary skill in the art will appreciate that wild type genes and polypeptides often exist in multiple different forms (e.g., alleles). 
     Nucleic acid: The term “nucleic acid” includes any nucleotides, analogs thereof, and polymers thereof. The term “polynucleotide” as used herein refer to a polymeric form of nucleotides of any length, either ribonucleotides (RNA) or deoxyribonucleotides (DNA). These terms refer to the primary structure of the molecules and, thus, include double- and single-stranded DNA, and double- and single-stranded RNA. These terms include, as equivalents, analogs of either RNA or DNA made from nucleotide analogs and modified polynucleotides such as, though not limited to, methylated, protected and/or capped nucleotides or polynucleotides. The terms encompass poly- or oligo-ribonucleotides (RNA) and poly- or oligo-deoxyribonucleotides (DNA); RNA or DNA derived from N-glycosides or C-glycosides of nucleobases and/or modified nucleobases; nucleic acids derived from sugars and/or modified sugars; and nucleic acids derived from phosphate bridges and/or modified phosphorus-atom bridges (also referred to herein as “internucleotide linkages”). The term encompasses nucleic acids containing any combinations of nucleobases, modified nucleobases, sugars, modified sugars, phosphate bridges or modified phosphorus atom bridges. Examples include, and are not limited to, nucleic acids containing ribose moieties, the nucleic acids containing deoxy-ribose moieties, nucleic acids containing both ribose and deoxyribose moieties, nucleic acids containing ribose and modified ribose moieties. The prefix poly- refers to a nucleic acid containing 2 to about 10,000 nucleotide monomer units and wherein the prefix oligo- refers to a nucleic acid containing 2 to about 200 nucleotide monomer units. 
     Nucleotide: The term “nucleotide” as used herein refers to a monomeric unit of a polynucleotide that consists of a heterocyclic base, a sugar, and one or more phosphate groups or phosphorus-containing internucleotidic linkages. The naturally occurring bases, (guanine, (G), adenine, (A), cytosine, (C), thymine, (T), and uracil (U)) are derivatives of purine or pyrimidine, though it should be understood that naturally and non-naturally occurring base analogs are also included. The naturally occurring sugar is the pentose (five-carbon sugar) deoxyribose (which forms DNA) or ribose (which forms RNA), though it should be understood that naturally and non-naturally occurring sugar analogs are also included. Nucleotides are linked via internucleotidic linkages to form nucleic acids, or polynucleotides. Many internucleotidic linkages are known in the art (such as, though not limited to, phosphate, phosphorothioates, boranophosphates and the like). Artificial nucleic acids include PNAs (peptide nucleic acids), phosphotriesters, phosphorothionates, H-phosphonates, phosphoramidates, boranophosphates, methylphosphonates, phosphonoacetates, thiophosphonoacetates and other variants of the phosphate backbone of native nucleic acids, such as those described herein. 
     Nucleoside: The term “nucleoside” refers to a moiety wherein a nucleobase or a modified nucleobase is covalently bound to a sugar or modified sugar. 
     Sugar: The term “sugar” refers to a monosaccharide in closed and/or open form. Sugars include, but are not limited to, ribose, deoxyribose, pentofuranose, pentopyranose, and hexopyranose moieties. As used herein, the term also encompasses structural analogs used in lieu of conventional sugar molecules, such as glycol, polymer of which forms the backbone of the nucleic acid analog, glycol nucleic acid (“GNA”). 
     Modified sugar: The term “modified sugar” refers to a moiety that can replace a sugar. The modified sugar mimics the spatial arrangement, electronic properties, or some other physicochemical property of a sugar. 
     Nucleobase: The term “nucleobase” refers to the parts of nucleic acids that are involved in the hydrogen-bonding that binds one nucleic acid strand to another complementary strand in a sequence specific manner. The most common naturally-occurring nucleobases are adenine (A), guanine (G), uracil (U), cytosine (C), and thymine (T). In some embodiments, the naturally-occurring nucleobases are modified adenine, guanine, uracil, cytosine, or thymine. In some embodiments, the naturally-occurring nucleobases are methylated adenine, guanine, uracil, cytosine, or thymine. In some embodiments, a nucleobase is a “modified nucleobase,” e.g., a nucleobase other than adenine (A), guanine (G), uracil (U), cytosine (C), and thymine (T). In some embodiments, the modified nucleobases are methylated adenine, guanine, uracil, cytosine, or thymine. In some embodiments, the modified nucleobase mimics the spatial arrangement, electronic properties, or some other physicochemical property of the nucleobase and retains the property of hydrogen-bonding that binds one nucleic acid strand to another in a sequence specific manner. In some embodiments, a modified nucleobase can pair with all of the five naturally occurring bases (uracil, thymine, adenine, cytosine, or guanine) without substantially affecting the melting behavior, recognition by intracellular enzymes or activity of the oligonucleotide duplex. 
     Chiral ligand: The term “chiral ligand” or “chiral auxiliary” refers to a moiety that is chiral and can be incorporated into a reaction so that the reaction can be carried out with certain stereoselectivity. 
     Condensing reagent: In a condensation reaction, the term “condensing reagent” refers to a reagent that activates a less reactive site and renders it more susceptible to attack by another reagent. In some embodiments, such another reagent is a nucleophile. 
     Blocking group: The term “blocking group” refers to a group that masks the reactivity of a functional group. The functional group can be subsequently unmasked by removal of the blocking group. In some embodiments, a blocking group is a protecting group. 
     Moiety: The term “moiety” refers to a specific segment or functional group of a molecule. Chemical moieties are often recognized chemical entities embedded in or appended to a molecule. 
     Solid support: The term “solid support” refers to any support which enables synthesis of nucleic acids. In some embodiments, the term refers to a glass or a polymer, that is insoluble in the media employed in the reaction steps performed to synthesize nucleic acids, and is derivatized to comprise reactive groups. In some embodiments, the solid support is Highly Cross-linked Polystyrene (HCP) or Controlled Pore Glass (CPG). In some embodiments, the solid support is Controlled Pore Glass (CPG). In some embodiments, the solid support is hybrid support of Controlled Pore Glass (CPG) and Highly Cross-linked Polystyrene (HCP). 
     Linking moiety: The term “linking moiety” refers to any moiety optionally positioned between the terminal nucleoside and the solid support or between the terminal nucleoside and another nucleoside, nucleotide, or nucleic acid. 
     DNA molecule: A “DNA molecule” refers to the polymeric form of deoxyribonucleotides (adenine, guanine, thymine, or cytosine) in its either single stranded form or a double-stranded helix. This term refers only to the primary and secondary structure of the molecule, and does not limit it to any particular tertiary forms. Thus, this term includes double-stranded DNA found, inter alia, in linear DNA molecules (e.g., restriction fragments), viruses, plasmids, and chromosomes. In discussing the structure of particular double-stranded DNA molecules, sequences can be described herein according to the normal convention of giving only the sequence in the 5′ to 3′ direction along the non-transcribed strand of DNA (i.e., the strand having a sequence homologous to the mRNA). 
     Coding sequence: A DNA “coding sequence” or “coding region” is a double-stranded DNA sequence which is transcribed and translated into a polypeptide in vivo when placed under the control of appropriate expression control sequences. The boundaries of the coding sequence (the “open reading frame” or “ORF”) are determined by a start codon at the 5′ (amino) terminus and a translation stop codon at the 3′ (carboxyl) terminus. A coding sequence can include, but is not limited to, prokaryotic sequences, cDNA from eukaryotic mRNA, genomic DNA sequences from eukaryotic (e.g., mammalian) DNA, and synthetic DNA sequences. A polyadenylation signal and transcription termination sequence is, usually, be located 3′ to the coding sequence. The term “non-coding sequence” or “non-coding region” refers to regions of a polynucleotide sequence that are not translated into amino acids (e.g. 5′ and 3′ un-translated regions). 
     Reading frame: The term “reading frame” refers to one of the six possible reading frames, three in each direction, of the double stranded DNA molecule. The reading frame that is used determines which codons are used to encode amino acids within the coding sequence of a DNA molecule. 
     Antisense: As used herein, an “antisense” nucleic acid molecule comprises a nucleotide sequence which is complementary to a “sense” nucleic acid encoding a protein, e.g., complementary to the coding strand of a double-stranded cDNA molecule, complementary to an mRNA sequence or complementary to the coding strand of a gene. Accordingly, an antisense nucleic acid molecule can associate via hydrogen bonds to a sense nucleic acid molecule. 
     Wobble position: As used herein, a “wobble position” refers to the third position of a codon. Mutations in a DNA molecule within the wobble position of a codon, in some embodiments, result in silent or conservative mutations at the amino acid level. For example, there are four codons that encode Glycine, i.e., GGU, GGC, GGA and GGG, thus mutation of any wobble position nucleotide, to any other nucleotide selected from A, U, C and G, does not result in a change at the amino acid level of the encoded protein and, therefore, is a silent substitution. 
     Silent substitution: a “silent substitution” or “silent mutation” is one in which a nucleotide within a codon is modified, but does not result in a change in the amino acid residue encoded by the codon. Examples include mutations in the third position of a codon, as well in the first position of certain codons such as in the codon “CGG” which, when mutated to AGG, still encodes Arg. 
     Gene: The terms “gene,” “recombinant gene” and “gene construct” as used herein, refer to a DNA molecule, or portion of a DNA molecule, that encodes a protein or a portion thereof. The DNA molecule can contain an open reading frame encoding the protein (as exon sequences) and can further include intron sequences. The term “intron” as used herein, refers to a DNA sequence present in a given gene which is not translated into protein and is found in some, but not all cases, between exons. It can be desirable for the gene to be operably linked to, (or it can comprise), one or more promoters, enhancers, repressors and/or other regulatory sequences to modulate the activity or expression of the gene, as is well known in the art. 
     Complementary DNA: As used herein, a “complementary DNA” or “cDNA” includes recombinant polynucleotides synthesized by reverse transcription of mRNA and from which intervening sequences (introns) have been removed. 
     Homology: “Homology” or “identity” or “similarity” refers to sequence similarity between two nucleic acid molecules. Homology and identity can each be determined by comparing a position in each sequence which can be aligned for purposes of comparison. When an equivalent position in the compared sequences is occupied by the same base, then the molecules are identical at that position; when the equivalent site occupied by the same or a similar nucleic acid residue (e.g., similar in steric and/or electronic nature), then the molecules can be referred to as homologous (similar) at that position. Expression as a percentage of homology/similarity or identity refers to a function of the number of identical or similar nucleic acids at positions shared by the compared sequences. A sequence which is “unrelated” or “non-homologous” shares less than 40% identity, less than 35% identity, less than 30% identity, or less than 25% identity with a sequence described herein. In comparing two sequences, the absence of residues (amino acids or nucleic acids) or presence of extra residues also decreases the identity and homology/similarity. 
     In some embodiments, the term “homology” describes a mathematically based comparison of sequence similarities which is used to identify genes with similar functions or motifs. The nucleic acid sequences described herein can be used as a “query sequence” to perform a search against public databases, for example, to identify other family members, related sequences or homologs. In some embodiments, such searches can be performed using the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. (1990) J. Mol. Biol. 215:403-10. In some embodiments, BLAST nucleotide searches can be performed with the NBLAST program, score=100, wordlength=12 to obtain nucleotide sequences homologous to nucleic acid molecules of the invention. In some embodiments, to obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., (1997) Nucleic Acids Res. 25(17):3389-3402. When utilizing BLAST and Gapped BLAST programs, the default parameters of the respective programs (e.g., XBLAST and BLAST) can be used (See www.ncbi.nlm.nih.gov). 
     Identity: As used herein, “identity” means the percentage of identical nucleotide residues at corresponding positions in two or more sequences when the sequences are aligned to maximize sequence matching, i.e., taking into account gaps and insertions. Identity can be readily calculated by known methods, including but not limited to those described in (Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 1993; Computer Analysis of Sequence Data, Part I, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991; and Carillo, H., and Lipman, D., SIAM J. Applied Math., 48: 1073 (1988). Methods to determine identity are designed to give the largest match between the sequences tested. Moreover, methods to determine identity are codified in publicly available computer programs. Computer program methods to determine identity between two sequences include, but are not limited to, the GCG program package (Devereux, J., et al., Nucleic Acids Research 12(1): 387 (1984)), BLASTP, BLASTN, and FASTA (Altschul, S. F. et al., J. Molec. Biol. 215: 403-410 (1990) and Altschul et al. Nuc. Acids Res. 25: 3389-3402 (1997)). The BLAST X program is publicly available from NCBI and other sources (BLAST Manual, Altschul, S., et al., NCBI NLM NIH Bethesda, Md. 20894; Altschul, S., et al., J. Mol. Biol. 215: 403-410 (1990). The well-known Smith Waterman algorithm can also be used to determine identity. 
     Heterologous: A “heterologous” region of a DNA sequence is an identifiable segment of DNA within a larger DNA sequence that is not found in association with the larger sequence in nature. Thus, when the heterologous region encodes a mammalian gene, the gene can usually be flanked by DNA that does not flank the mammalian genomic DNA in the genome of the source organism. Another example of a heterologous coding sequence is a sequence where the coding sequence itself is not found in nature (e.g., a cDNA where the genomic coding sequence contains introns or synthetic sequences having codons or motifs different than the unmodified gene). Allelic variations or naturally-occurring mutational events do not give rise to a heterologous region of DNA as defined herein. 
     Transition mutation: The term “transition mutations” refers to base changes in a DNA sequence in which a pyrimidine (cytidine (C) or thymidine (T) is replaced by another pyrimidine, or a purine (adenosine (A) or guanosine (G) is replaced by another purine. 
     Transversion mutation: The term “transversion mutations” refers to base changes in a DNA sequence in which a pyrimidine (cytidine (C) or thymidine (T) is replaced by a purine (adenosine (A) or guanosine (G), or a purine is replaced by a pyrimidine. 
     Oligonucleotide: the term “oligonucleotide” refers to a polymer or oligomer of nucleotide monomers, containing any combination of nucleobases, modified nucleobases, sugars, modified sugars, phosphate bridges, or modified phosphorus atom bridges (also referred to herein as “internucleotidic linkage”, defined further herein). 
     Oligonucleotides can be single-stranded or double-stranded. As used herein, the term “oligonucleotide strand” encompasses a single-stranded oligonucleotide. A single-stranded oligonucleotide can have double-stranded regions and a double-stranded oligonucleotide can have single-stranded regions. Exemplary oligonucleotides include, but are not limited to structural genes, genes including control and termination regions, self-replicating systems such as viral or plasmid DNA, single-stranded and double-stranded siRNAs and other RNA interference reagents (RNAi agents or iRNA agents), shRNA, antisense oligonucleotides, ribozymes, microRNAs, microRNA mimics, supermirs, aptamers, antimirs, antagomirs, U adaptors, triplex-forming oligonucleotides, G-quadruplex oligonucleotides, RNA activators, immuno-stimulatory oligonucleotides, and decoy oligonucleotides. 
     Double-stranded and single-stranded oligonucleotides that are effective in inducing RNA interference are also referred to as siRNA, RNAi agent, or iRNA agent, herein. In some embodiments, these RNA interference inducing oligonucleotides associate with a cytoplasmic multi-protein complex known as RNAi-induced silencing complex (RISC). In many embodiments, single-stranded and double-stranded RNAi agents are sufficiently long that they can be cleaved by an endogenous molecule, e.g., by Dicer, to produce smaller oligonucleotides that can enter the RISC machinery and participate in RISC mediated cleavage of a target sequence, e.g. a target mRNA. 
     Oligonucleotides of the present invention can be of various lengths. In particular embodiments, oligonucleotides can range from about 2 to about 200 nucleotides in length. In various related embodiments, oligonucleotides, single-stranded, double-stranded, and triple-stranded, can range in length from about 4 to about 10 nucleotides, from about 10 to about 50 nucleotides, from about 20 to about 50 nucleotides, from about 15 to about 30 nucleotides, from about 20 to about 30 nucleotides in length. In some embodiments, the oligonucleotide is from about 9 to about 39 nucleotides in length. In some embodiments, the oligonucleotide is at least 4 nucleotides in length. In some embodiments, the oligonucleotide is at least 5 nucleotides in length. In some embodiments, the oligonucleotide is at least 6 nucleotides in length. In some embodiments, the oligonucleotide is at least 7 nucleotides in length. In some embodiments, the oligonucleotide is at least 8 nucleotides in length. In some embodiments, the oligonucleotide is at least 9 nucleotides in length. In some embodiments, the oligonucleotide is at least 10 nucleotides in length. In some embodiments, the oligonucleotide is at least 11 nucleotides in length. In some embodiments, the oligonucleotide is at least 12 nucleotides in length. In some embodiments, the oligonucleotide is at least 15 nucleotides in length. In some embodiments, the oligonucleotide is at least 20 nucleotides in length. In some embodiments, the oligonucleotide is at least 25 nucleotides in length. In some embodiments, the oligonucleotide is at least 30 nucleotides in length. In some embodiments, the oligonucleotide is a duplex of complementary strands of at least 18 nucleotides in length. In some embodiments, the oligonucleotide is a duplex of complementary strands of at least 21 nucleotides in length. 
     Internucleotidic linkage: As used herein, the phrase “internucleotidic linkage” refers generally to the phosphorus-containing linkage between nucleotide units of an oligonucleotide, and is interchangeable with “inter-sugar linkage” and “phosphorus atom bridge,” as used above and herein. In some embodiments, an internucleotidic linkage is a phosphodiester linkage, as found in naturally occurring DNA and RNA molecules. In some embodiments, an internucleotidic linkage is a “modified internucleotidic linkage” wherein each oxygen atom of the phosphodiester linkage is optionally and independently replaced by an organic or inorganic moiety. In some embodiments, such an organic or inorganic moiety is selected from but not limited to ═S, ═Se, ═NR′, —SR′, —SeR′, —N(R′) 2 , B(R′) 3 , —S—, —Se—, and —N(R′)—, wherein each R′ is independently as defined and described below. In some embodiments, an internucleotidic linkage is a phosphotriester linkage, phosphorothioate diester linkage 
                         
or modified phosphorothioate triester linkage. It is understood by a person of ordinary skill in the art that the internucleotidic linkage may exist as an anion or cation at a given pH due to the existence of acid or base moieties in the linkage.
 
     Unless otherwise specified, when used with an oligonucleotide sequence, each of s, s1, s2, s3, s4, s5, s6 and s7 independently represents the following modified internucleotidic linkage as illustrated in Table 1, below. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Exemplary Modified Internucleotidic Linkage. 
               
            
           
           
               
               
            
               
                 Sym- 
                   
               
               
                 bol 
                 Modified Internucleotidic Linkage 
               
               
                   
               
               
                 s 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s1 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s2 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s3 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s4 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s5 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s6 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s7 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s8 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s9 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s10 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s11 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s12 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s13 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s14 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s15 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s16 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s17 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 s18 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     For instance, (Rp, Sp)-ATsCs1GA has 1) a phosphorothioate internucleotidic linkage 
                         
between T and C; and 2) a phosphorothioate triester internucleotidic linkage having the structure of
 
                         
between C and G. Unless otherwise specified, the Rp/Sp designations preceding an oligonucleotide sequence describe the configurations of chiral linkage phosphorus atoms in the internucleotidic linkages sequentially from 5′ to 3′ of the oligonucleotide sequence. For instance, in (Rp, Sp)-ATsCs1GA, the phosphorus in the “s” linkage between T and C has Rp configuration and the phosphorus in “s1” linkage between C and G has Sp configuration. In some embodiments, “All-(Rp)” or “All-(Sp)” is used to indicate that all chiral linkage phosphorus atoms in oligonucleotide have the same Rp or Sp configuration, respectively. For instance, All-(Rp)-GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC (SEQ ID NO: 106) indicates that all the chiral linkage phosphorus atoms in the oligonucleotide have Rp configuration; All-(Sp)-GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC (SEQ ID NO: 106) indicates that all the chiral linkage phosphorus atoms in the oligonucleotide have Sp configuration.
 
     Oligonucleotide type: As used herein, the phrase “oligonucleotide type” is used to define an oligonucleotide that has a particular base sequence, pattern of backbone linkages (i.e., pattern of internucleotidic linkage types, for example, phosphate, phosphorothioate, etc), pattern of backbone chiral centers (i.e. pattern of linkage phosphorus stereochemistry (Rp/Sp)), and pattern of backbone phosphorus modifications (e.g., pattern of “—XLR 1 ” groups in formula I). Oligonucleotides of a common designated “type” are structurally identical to one another. 
     One of skill in the art will appreciate that synthetic methods of the present invention provide for a degree of control during the synthesis of an oligonucleotide strand such that each nucleotide unit of the oligonucleotide strand can be designed and/or selected in advance to have a particular stereochemistry at the linkage phosphorus and/or a particular modification at the linkage phosphorus, and/or a particular base, and/or a particular sugar. In some embodiments, an oligonucleotide strand is designed and/or selected in advance to have a particular combination of stereocenters at the linkage phosphorus. In some embodiments, an oligonucleotide strand is designed and/or determined to have a particular combination of modifications at the linkage phosphorus. In some embodiments, an oligonucleotide strand is designed and/or selected to have a particular combination of bases. In some embodiments, an oligonucleotide strand is designed and/or selected to have a particular combination of one or more of the above structural characteristics. The present invention provides compositions comprising or consisting of a plurality of oligonucleotide molecules (e.g., chirally controlled oligonucleotide compositions). In some embodiments, all such molecules are of the same type (i.e., are structurally identical to one another). In many embodiments, however, provided compositions comprise a plurality of oligonucleotides of different types, typically in pre-determined relative amounts. 
     Chiral control: As used herein, “chiral control” refers to an ability to control the stereochemical designation of every chiral linkage phosphorus within an oligonucleotide strand. The phrase “chirally controlled oligonucleotide” refers to an oligonucleotide which exists in a single diastereomeric form with respect to the chiral linkage phosphorus. 
     Chirally controlled oligonucleotide composition: As used herein, the phrase “chirally controlled oligonucleotide composition” refers to an oligonucleotide composition that contains predetermined levels of individual oligonucleotide types. For instance, in some embodiments a chirally controlled oligonucleotide composition comprises one oligonucleotide type. In some embodiments, a chirally controlled oligonucleotide composition comprises more than one oligonucleotide type. In some embodiments, a chirally controlled oligonucleotide composition comprises a mixture of multiple oligonucleotide types. Exemplary chirally controlled oligonucleotide compositions are described further herein. 
     Chirally pure: as used herein, the phrase “chirally pure” is used to describe a chirally controlled oligonucleotide composition in which all of the oligonucleotides exist in a single diastereomeric form with respect to the linkage phosphorus. 
     Chirally uniform: as used herein, the phrase “chirally uniform” is used to describe an oligonucleotide molecule or type in which all nucleotide units have the same stereochemistry at the linkage phosphorus. For instance, an oligonucleotide whose nucleotide units all have Rp stereochemistry at the linkage phosphorus is chirally uniform. Likewise, an oligonucleotide whose nucleotide units all have Sp stereochemistry at the linkage phosphorus is chirally uniform. 
     Predetermined: By predetermined is meant deliberately selected, for example as opposed to randomly occurring or achieved. Those of ordinary skill in the art, reading the present specification, will appreciate that the present invention provides new and surprising technologies that permit selection of particular oligonucleotide types for preparation and/or inclusion in provided compositions, and further permits controlled preparation of precisely the selected particular types, optionally in selected particular relative amounts, so that provided compositions are prepared. Such provided compositions are “predetermined” as described herein. Compositions that may contain certain individual oligonucleotide types because they happen to have been generated through a process that cannot be controlled to intentionally generate the particular oligonucleotide types is not a “predetermined” composition. In some embodiments, a predetermined composition is one that can be intentionally reproduced (e.g., through repetition of a controlled process). 
     Linkage phosphorus: as defined herein, the phrase “linkage phosphorus” is used to indicate that the particular phosphorus atom being referred to is the phosphorus atom present in the internucleotidic linkage, which phosphorus atom corresponds to the phosphorus atom of a phosphodiester of an internucleotidic linkage as occurs in naturally occurring DNA and RNA. In some embodiments, a linkage phosphorus atom is in a modified internucleotidic linkage, wherein each oxygen atom of a phosphodiester linkage is optionally and independently replaced by an organic or inorganic moiety. In some embodiments, a linkage phosphorus atom is P* of formula I. In some embodiments, a linkage phosphorus atom is chiral. In some embodiments, a chiral linkage phosphorus atom is P* of formula I. 
     P-modification: as used herein, the term “P-modification” refers to any modification at the linkage phosphorus other than a stereochemical modification. In some embodiments, a P-modification comprises addition, substitution, or removal of a pendant moiety covalently attached to a linkage phosphorus. In some embodiments, the “P-modification” is —X-L-R 1  wherein each of X, L and R 1  is independently as defined and described herein and below. 
     Blockmer: the term “blockmer,” as used herein, refers to an oligonucleotide strand whose pattern of structural features characterizing each individual nucleotide unit is characterized by the presence of at least two consecutive nucleotide units sharing a common structural feature at the internucleotidic phosphorus linkage. By common structural feature is meant common stereochemistry at the linkage phosphorus or a common modification at the linkage phosphorus. In some embodiments, the at least two consecutive nucleotide units sharing a common structure feature at the internucleotidic phosphorus linkage are referred to as a “block”. 
     In some embodiments, a blockmer is a “stereoblockmer,” e.g., at least two consecutive nucleotide units have the same stereochemistry at the linkage phosphorus. Such at lest two consecutive nucleotide units form a “stereoblock.” For instance, (Sp, Sp)-ATsCs1GA is a stereoblockmer because at least two consecutive nucleotide units, the Ts and the Cs1, have the same stereochemistry at the linkage phosphorus (both Sp). In the same oligonucleotide (Sp, Sp)-ATsCs1GA, TsCs1 forms a block, and it is a stereoblock. 
     In some embodiments, a blockmer is a “P-modification blockmer,” e.g., at least two consecutive nucleotide units have the same modification at the linkage phosphorus. Such at lest two consecutive nucleotide units form a “P-modification block”. For instance, (Rp, Sp)-ATsCsGA is a P-modification blockmer because at least two consecutive nucleotide units, the Ts and the Cs, have the same P-modification (i.e., both are a phosphorothioate diester). In the same oligonucleotide of (Rp, Sp)-ATsCsGA, TsCs forms a block, and it is a P-modification block. 
     In some embodiments, a blockmer is a “linkage blockmer,” e.g., at least two consecutive nucleotide units have identical stereochemistry and identical modifications at the linkage phosphorus. At least two consecutive nucleotide units form a “linkage block”. For instance, (Rp, Rp)-ATsCsGA is a linkage blockmer because at least two consecutive nucleotide units, the Ts and the Cs, have the same stereochemistry (both Rp) and P-modification (both phosphorothioate). In the same oligonucleotide of (Rp, Rp)-ATsCsGA, TsCs forms a block, and it is a linkage block. 
     In some embodiments, a blockmer comprises one or more blocks independently selected from a stereoblock, a P-modification block and a linkage block. In some embodiments, a blockmer is a stereoblockmer with respect to one block, and/or a P-modification blockmer with respect to another block, and/or a linkage blockmer with respect to yet another block. For instance, (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp)-AAsTsCsGsAs1Ts1Cs1Gs1ATCG (SEQ ID NO: 107) is a stereoblockmer with respect to the stereoblock AsTsCsGsAs1 (all Rp at linkage phosphorus) or Ts1Cs1Gs1 (all Sp at linkage phosphorus), a P-modification blockmer with respect to the P-modification block AsTsCsGs (all s linkage) or As1Ts1Cs1Gs1 (all s1 linkage), or a linkage blockmer with respect to the linkage block AsTsCsGs (all Rp at linkage phosphorus and all s linkage) or Ts1Cs1Gs1 (all Sp at linkage phosphorus and all s1 linkage). 
     Altmer: the term “altmer,” as used herein, refers to an oligonucleotide strand whose pattern of structural features characterizing each individual nucleotide unit is characterized in that no two consecutive nucleotide units of the oligonucleotide strand share a particular structural feature at the internucleotidic phosphorus linkage. In some embodiments, an altmer is designed such that it comprises a repeating pattern. In some embodiments, an altmer is designed such that it does not comprise a repeating pattern. 
     In some embodiments, an altmer is a “stereoaltmer,” e.g., no two consecutive nucleotide units have the same stereochemistry at the linkage phosphorus. For instance, (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp)-GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC (SEQ ID NO: 106). 
     In some embodiments, an altmer is a “P-modification altmer” e.g., no two consecutive nucleotide units have the same modification at the linkage phosphorus. For instance, All-(Sp)-CAs1GsT, in which each linkage phosphorus has a different P-modification than the others. 
     In some embodiments, an altmer is a “linkage altmer,” e.g., no two consecutive nucleotide units have identical stereochemistry or identical modifications at the linkage phosphorus. For instance, (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp)-GsCs1CsTs1CsAs1GsTs1CsTs1GsCs1TsTs2CsGs3CsAs4CsC (SEQ ID NO: 106). 
     Unimer: the term “unimer,” as used herein, refers to an oligonucleotide strand whose pattern of structural features characterizing each individual nucleotide unit is such that all nucleotide units within the strand share at least one common structural feature at the internucleotidic phosphorus linkage. By common structural feature is meant common stereochemistry at the linkage phosphorus or a common modification at the linkage phosphorus. 
     In some embodiments, a unimer is a “stereounimer,” e.g., all nucleotide units have the same stereochemistry at the linkage phosphorus. For instance, All-(Sp)-CsAs1GsT, in which all the linkages have Sp phosphorus. 
     In some embodiments, a unimer is a “P-modification unimer”, e.g., all nucleotide units have the same modification at the linkage phosphorus. For instance, (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp)-GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC (SEQ ID NO: 106), in which all the internucleotidic linkages are phosphorothioate diester. 
     In some embodiments, a unimer is a “linkage unimer,” e.g., all nucleotide units have the same stereochemistry and the same modifications at the linkage phosphorus. For instance, All-(Sp)-GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC (SEQ ID NO: 106), in which all the internucleotidic linkages are phosphorothioate diester having Sp linkage phosphorus. 
     Gapmer: as used herein, the term “gapmer” refers to an oligonucleotide strand characterized in that at least one internucleotidic phosphorus linkage of the oligonucleotide strand is a phosphate diester linkage, for example such as those found in naturally occurring DNA or RNA. In some embodiments, more than one internucleotidic phosphorus linkage of the oligonucleotide strand is a phosphate diester linkage such as those found in naturally occurring DNA or RNA. For instance, All-(Sp)-CAs1GsT, in which the internucleotidic linkage between C and A is a phosphate diester linkage. 
     Skipmer: as used herein, the term “skipmer” refers to a type of gapmer in which every other internucleotidic phosphorus linkage of the oligonucleotide strand is a phosphate diester linkage, for example such as those found in naturally occurring DNA or RNA, and every other internucleotidic phosphorus linkage of the oligonucleotide strand is a modified internucleotidic linkage. For instance, All-(Sp)-AsTCs1GAs2TCs3G. 
     For purposes of this invention, the chemical elements are identified in accordance with the Periodic Table of the Elements, CAS version,  Handbook of Chemistry and Physics,  67th Ed., 1986-87, inside cover. 
     The methods and structures described herein relating to compounds and compositions of the invention also apply to the pharmaceutically acceptable acid or base addition salts and all stereoisomeric forms of these compounds and compositions. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWING 
         FIG.  1   . Chirally controlled oligonucleotide has significantly different retention time on HPLC compared to the stereorandom oligonucleotide. A: crude chirally controlled oligonucleotide (Oligonucleotide 101); C: the corresponding stereorandom oligonucleotide (Oligonucleotide 118). 
         FIG.  2   . HPLC of chirally controlled oligonucleotides and stereorandom oligonucleotide. A: Oligonucleotide 101 (all-Rp); B: Oligonucleotide 102 (all-Sp); and C: Oligonucleotide 118 (stereorandom). 
         FIG.  3   . Tm of chirally controlled oligonucleotides and stereorandom oligonucleotide. 
         FIG.  4   . Representative Data: Melting Curve Analysis of the target and endogenous control pairs yield single amplicons. 
         FIG.  5   . Representative data and IC 50  curves for compounds. 
         FIG.  6   . HPLC of crude (Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] ((RRS) 6 -R (SEQ ID NO: 106), stereoblockmer and P-modification unimer (s-unimer)). 
         FIG.  7   . HPLC of purified (Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] ((RRS) 6 -R (SEQ ID NO: 106), stereoblockmer and P-modification unimer (s-unimer)). 
         FIG.  8   . LCMS of (Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] ((RRS) 6 -R (SEQ ID NO: 106), stereoblockmer and P-modification unimer (s-unimer)). 
         FIG.  9   . HPLC of crude (Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (S-(RRS) 6  (SEQ ID NO: 106), stereoblockmer and P-modification unimer (s-unimer)). 
         FIG.  10   . HPLC of purified (Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (S-(RRS) 6  (SEQ ID NO: 106), stereoblockmer and P-modification unimer (s-unimer)). 
         FIG.  11   . LCMS of (Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (S-(RRS) 6  (SEQ ID NO: 106), stereoblockmer and P-modification unimer (s-unimer)). 
         FIG.  12   . HPLC of crude (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp) d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (RS-(RRS) 5 -RR (SEQ ID NO: 106), stereoblockmer and P-modification unimer (s-unimer)). 
         FIG.  13   . HPLC of purified (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp) d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (RS-(RRS) 5 -RR (SEQ ID NO: 106), stereoblockmer and P-modification unimer (s-unimer)). 
         FIG.  14   . LCMS of (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (RS-(RRS) 5 -RR (SEQ ID NO: 106), stereoblockmer and P-modification unimer (s-unimer)). 
         FIG.  15   . HPLC of crude (Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) (3R-5S-3R, stereoblockmer and P-modification unimer (s1-unimer)). 
         FIG.  16   . HPLC of purified (Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) (3R-5S-3R, stereoblockmer and P-modification unimer (s1-unimer)). 
         FIG.  17   . LCMS of (Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) (3R-5S-3R, stereoblockmer and P-modification unimer (s1-unimer)). 
         FIG.  18   . HPLC of crude All-(Rp)-d[Cs3As3Gs3T] (P-modification unimer (s3-unimer), stereounimer and linkage unimer). 
         FIG.  19   . LCMS of All-(Rp)-d[Cs3As3Gs3T] (P-modification unimer (s3-unimer), stereounimer and linkage unimer). 
         FIG.  20   . HPLC of crude All-(Rp)-d[Cs2As2Gs2T] (P-modification unimer (s2-unimer), stereounimer and linkage unimer). 
         FIG.  21   . LCMS of All-(Rp)-d[Cs2As2Gs2T] (P-modification unimer (s2-unimer), stereounimer and linkage unimer). 
         FIG.  22   . HPLC of crude All-(Sp)-d[Cs1AGs1T] (gapmer, stereoaltmer, P-modification altmer and linkage altmer). 
         FIG.  23   . LCMS of All-(Sp)-d[Cs1AGs1T] (gapmer stereoaltmer, P-modification altmer and linkage altmer). 
         FIG.  24   . Crude All-(Rp)-d[TsCs1AsT] (stereounimer, P-modification altmer and linkage altmer). 
         FIG.  25   . LCMS of All-(Rp)-d[TsCs1AsT] (stereounimer, P-modification altmer and linkage altmer). 
         FIG.  26   . Exemplary oligonucleotides (SEQ ID NOS 133 and 133, respectively, in order of appearance) described in WO2012/030683 and contemplated for synthesis using methods of the present invention. 
         FIG.  27   . Exemplary oligonucleotides (SEQ ID NOS 134-135, 135, 134, 136 and 135, respectively, in order of appearance) described in WO2012/030683 and contemplated for synthesis using methods of the present invention. 
         FIG.  28   . Exemplary oligonucleotides (SEQ ID NOS 135, 135, 135, 135, 135 and 137, respectively, in order of appearance) described in WO2012/030683 and contemplated for synthesis using methods of the present invention. 
         FIG.  29   . Exemplary oligonucleotides (SEQ ID NOS 136, 138, 136 and 137, respectively, in order of appearance) described in WO2012/030683 and contemplated for synthesis using methods of the present invention. 
         FIG.  30   . Exemplary oligonucleotides (SEQ ID NOS 134, 133 and 133, respectively, in order of appearance) described in WO2012/030683 and contemplated for synthesis using methods of the present invention. 
         FIG.  31   . Exemplary linkers described in WO2012/030683 for use in methods of the present invention. 
         FIG.  32   . Exemplary linkers described in WO2012/030683 for use in methods of the present invention. 
         FIG.  33   . Exemplary linkers described in WO2012/030683 for use in methods of the present invention. 
         FIG.  34   . Exemplary linkers described in WO2012/030683 for use in methods of the present invention. 
         FIG.  35   . RP-HPLC of crude DMT on oligonucleotide: ONT-75 (Panel A); ONT-80 (Panel B); ONT-77 (Panel C); ONT-81 (Panel D); ONT-87 (Panel E); ONT-88 (Panel F); ONT-89 (Panel G); ONT-82 (Panel H); ONT-84 (Panel I); ONT-85 (Panel J); ONT-86 (Panel K). 
         FIG.  36   . RP-HPLC of purified DMT off oligonucleotide: ONT-75 (Panel A); ONT-80 (Panel B); ONT-77 (Panel C); ONT-81 (Panel D); ONT-87 (Panel E); ONT-88 (Panel F); ONT-89 (Panel G); ONT-82 (Panel H); ONT-84 (Panel I); ONT-85 (Panel J); ONT-86 (Panel K). 
         FIG.  37   . Overlay of RP-HPLC traces of purified DMT off oligonucleotide: ONT-75, ONT-77, ONT-80, ONT-81, ONT-87, ONT-88, ONT-89, and ONT-41 (Panel A); expanded view of overlay of ONT-75, ONT-77, ONT-80, ONT-81, ONT-87, ONT-88, ONT-89, and ONT-41 (Panel B). 
         FIG.  38   . Overlay of RP-HPLC traces of purified DMT off oligonucleotide: ONT-82, ONT-84, ONT-85, ONT-86, and ONT-83 (Panel A); expanded view of overlay of ONT-82, ONT-84, ONT-85, ONT-86, and ONT-83 (Panel B). 
         FIG.  39   . Tm overlay of chirally controlled oligonucleotides ONT-81, ONT-41, ONT-75, ONT-77, and ONT-80. 
         FIG.  40   . Graphical representation of timecourse of serum human apolipoprotein B protein levels relative to PBS after 5 mg/kg stereoisomer or mipomersen IP dosing in huApoB mice for ONT-41, ONT-75, ONT-80, ONT-77, and ONT-81. A downward arrow indicates dosing days. 
         FIG.  41   . Graphical representation of timecourse of serum human apolipoprotein B protein levels relative to PBS after 5 mg/kg stereoisomer or mipomersen IP dosing in huApoB mice for mipomersen, “full R” mipomersen, “full S” mipomersen, “RSR” mipomersen, and “SRS” mipomersen. A downward arrow indicates dosing days. 
         FIG.  42   . Graphical representation of timecourse of serum human apolipoprotein B protein levels relative to PBS after 10 mg/kg stereoisomer or mipomersen IP dosing in huApoB mice for mipomersen, “full R” mipomersen, “full S” mipomersen, “RSR” mipomersen, and “SRS” mipomersen. A downward arrow indicates dosing days. 
         FIG.  43   . Graphical representation of timecourse of serum human apolipoprotein B protein levels relative to PBS after 5 mg/kg stereoisomer or mipomersen IP dosing in huApoB mice for mipomersen, ONT-87, ONT-88, and ONT-89. A downward arrow indicates dosing days. 
         FIG.  44   . Graphical representation of timecourse of serum human apolipoprotein B protein levels relative to PBS after 10 mg/kg stereoisomer or mipomersen IP dosing in huApoB mice for ONT-87, ONT-88, and ONT-89. A downward arrow indicates dosing days. 
         FIG.  45   . Graphical representation of % PCSK-9 mRNA remaining after Hep3B treatment with siRNA duplex. 
         FIG.  46   . Graphical representation of % PCSK-9 mRNA remaining after Hep3B treatment with siRNA duplex curve fit. 
         FIG.  47   . Graphical representation of % PCSK-9 mRNA remaining after HeLa treatment with siRNA duplex. 
         FIG.  48   . Graphical representation of % PCSK-9 mRNA remaining after HeLa treatment with siRNA duplex curve fit. 
         FIG.  49   . Graphical representation of % PCSK-9 mRNA remaining after HeLa treatment with siRNA duplex containing 3 Phosphorothiate stereo-centers. 
         FIG.  50   . Graphical representation of % PCSK-9 mRNA remaining after HeLa treatment with siRNA duplex containing 3 Phosphorothiate stereo-centers curve fit. 
         FIG.  51   . Overlay of RP-HPLC traces of purified DMT off oligonucleotide: ONT-108, ONT-109, and ONT-114. 
         FIG.  52   . Overlay of RP-HPLC traces of purified DMT off oligonucleotide: ONT-106, ONT-107, and ONT-114. 
         FIG.  53   . Graphical representation of timecourse of serum human apolipoprotein B protein levels relative to PBS after 10 mg/kg stereoisomer or mipomersen IP dosing in huApoB mice. A downward arrow indicates dosing days. 
         FIG.  54   . Graphical representation of timecourse of serum human apolipoprotein B protein levels relative to PBS after multiple IP doses of 5 mg/kg stereoisomer or mipomersen in huApoB mice (n=3-4). A downward arrow indicates dosing days. 
         FIG.  55   . Day 17 serum human apolipoprotein B protein levels relative to PBS after 10 mg/kg stereoisomer (ONT-87, ONT-88 or ONT-89) or mipomersen IP dosing in huApoB mice. 
         FIG.  56   . Day 24 Serum Human Apolipoprotein B Protein Levels Relative to PBS After 10 mg/kg Stereoisomer (ONT-87, ONT-88 or ONT-89) or Mipomersen IP Dosing in huApoB Mice. 
         FIG.  57   . Serum Human Apolipoprotein B Protein Levels Relative to PBS After 10 mg/kg Stereoisomer (ONT-41, ONT-87, ONT-88 or ONT-89) Dosing in huApoB Mice. 
         FIG.  58   . Serum Human Apolipoprotein B Protein Levels Relative to PBS After 10 mg/kg Stereoisomer (ONT-87, ONT-88 or ONT-89) Dosing in huApoB Mice. 
         FIG.  59   . Plot of IEX-HPLC quantification analysis of svPDE digestion study for oligonucleotides ONT-75, ONT-77, ONT-80, ONT-81, ONT-87, ONT-88, ONT-89 and ONT-41. 
         FIG.  60   . IEX-HPLC of enzymatic digestion study using nP1 for oligonucleotide ONT-75 (All (Rp))- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsmCsTsTs5mCs Gs 5mCsAs5mCs5mC  (SEQ ID NO: 106). 
         FIG.  61   . IEX-HPLC of enzymatic digestion study using nP1 for oligonucleotide ONT-77 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp)- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) (5R-10S-4R). 
         FIG.  62   . IEX-HPLC of enzymatic digestion study using nP1 for oligonucleotide ONT-80 (All (Sp))- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsGs5mCsTsTs5mCs  Gs5mCsAs5mCs5mC  (SEQ ID NO: 106). 
         FIG.  63   . IEX-HPLC of enzymatic digestion study using nP1 for oligonucleotide ONT-81 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) (5S-10R-4S). 
         FIG.  64   . IEX-HPLC of enzymatic digestion study using nP1 for oligonucleotide ONT-87 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp)- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) (5R-(SSR) 3 -5R). 
         FIG.  65   . IEX-HPLC of enzymatic digestion study using nP1 for oligonucleotide ONT-88 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp)- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) (5S-(RRS) 3 -5SS). 
         FIG.  66   . IEX-HPLC of enzymatic digestion study using nP1 for oligonucleotide ONT-89 (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp)- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) ((SR) 9 S). 
         FIG.  67   . IEX-HPLC of enzymatic digestion study using nP1 for oligonucleotide ONT-41 (diastereomixture)- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsGs 5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106). 
         FIG.  68   . Comparison of stability of chirally pure oligonucleotides ONT-75 and ONT-77 with the stereorandom “parental” oligonucleotide ONT-41 (Mipomersen) in preincubated rat whole liver homogenate. 
         FIG.  69   . UPLC profile in producing oligonucleotide derivative using the monomer of 13b. 
         FIG.  70   . UPLC profile in producing oligonucleotide derivative using the monomer of 27. 
         FIG.  71   . Mouse Apolipoprotein B/GAPDH mRNA Levels Relative to Mock and Untreated Controls after Transfection of Primary Mouse Hepatocytes with Stereoisomer (ONT-82, ONT-83, ONT-84, ONT-85 or ONT-86). 
         FIG.  72   . Mouse Apolipoprotein B/GAPDH mRNA Levels Relative to Mock and Untreated Controls after Transfection of Primary Mouse Hepatocytes with Stereoisomer (ONT-83, ONT-84, ONT-85 or ONT-86). 
     
    
    
     DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS 
     Synthetic oligonucleotides provide useful molecular tools in a wide variety of applications. For example, oligonucleotides are useful in therapeutic, diagnostic, research, and new nanomaterials applications. The use of naturally occurring nucleic acids (e.g., unmodified DNA or RNA) is limited, for example, by their susceptibility to endo- and exo-nucleases. As such, various synthetic counterparts have been developed to circumvent these shortcomings. These include synthetic oligonucleotides that contain backbone modifications, which render these molecules less susceptible to degradation. From a structural point of view, such modifications to internucleotide phosphate linkages introduce chirality. It has become clear that certain properties of oligonucleotides may be affected by the configurations of the phosphorus atoms that form the backbone of the oligonucleotides. For example, in vitro studies have shown that the properties of antisense nucleotides such as binding affinity, sequence specific binding to the complementary RNA, stability to nucleases are affected by, inter alia, chirality of the backbone (e.g., the configurations of the phosphorus atoms). Thus, the present invention encompasses the recognition that there is a need for chirally controlled oligonucleotides which comprise phosphorus atom-modified nucleic acids, as well as related compositions and methods. In some embodiments, the present invention provides chirally controlled oligonucleotides that are structurally optimized to exhibit certain desirable characteristics, such as, e.g., increased stability and improved efficacy for in vitro and/or in vivo applications. 
     Oligonucleotides in which one or two of the two nonbridging oxygen atoms of the internucleotidic phosphates is replaced by a different type of atom or substituent are known to be useful as therapeutic agents and probes to elucidate enzymatic reaction mechanisms. However, such oligonucleotides often exhibit undesirable properties (e.g., susceptibility to degradation by nucleases, poor cell membrane permeability) that prohibit their use in numerous applications. Thus, various types of chemical modifications have been developed in an attempt to improve their properties and/or impart new functionality. 
     Modified Oligonucleotide Structures 
     As noted above, in light of the usefulness of oligonucleotide compositions in various applications and indications, those skilled in the art have endeavored to develop modifications of oligonucleotide structures that may have preferred or desirable characteristics or attributes as compared with naturally-occurring oligonucleotide molecules, for example as used in particular applications and indications. Exemplary such modifications are described below. 
     WO2010/141471 (herein “Traversa I”) teaches the modification of different types of nucleic acid constructs modified to have a reduced net polyanionic charge. WO2010/039543 (herein “Travera II”) teaches compositions and methods for making neutral polynucleotides (NNs) with reduced polyanionic charge. WO2008/008476 (herein, “Traversa III”) describes the synthesis of SATE (Imbach-type) phosphate prodrugs. Traversa I, II, and III do not teach chirally controlled oligonucleotides, compositions thereof, and methods of making and using the same, as described by the present invention. 
     WO2010/072831 (herein “Girindus et al.”) also teaches the modification of oligonucleotides. In particular, Girindus et al. teaches the use of sulfurization reagents to generate phosphorothioate triesters as prodrugs. Girindus et al. does not teach chirally controlled oligonucleotides, compositions thereof, and methods of making and using the same, as described by the present invention. 
     Similarly, WO2004/085454 (herein “Avecia I”) teaches the preparation of phosphorothioate oligonucleotides through, e.g., transient silylation of poly-H-phosphonate diesters. WO2001/027126 (herein “Avecia II”) teaches processes for the solid phase synthesis of phosphotriester oligonucleotides by coupling H-phosphonate monomers to a solid supported 5′-hydroxyl oligonucleotide and further sulfurization of the resulting H-phosphonate diester into a phosphorothioate triester. The disclosure of WO2001/064702 (herein “Avecia III”) is similar to Avecia II and further describes solid-phase synthesis on different solid supports. Avecia I, II, and III do not teach chirally controlled oligonucleotides, compositions thereof, and methods of making and using the same, as described by the present invention. 
     WO1997/006183 (herein “Chiron”) teaches oligonucleotides with cationic internucleotide linkages comprising asymmetric phosphorus, such as stereopure amidates. Chiron teaches stereopure oligonucleotides obtained via crystallization of a mixture of diastereomers or via resolution using, e.g., column chromatography. Chiron does not teach chirally controlled oligonucleotides, compositions thereof, and methods of making and using the same, as described by the present invention. 
     WO2009/146123 (herein “Spring Bank I”) teaches compositions and methods for treating viral infections using substituted phosphate oligonucleotides and phosphorothioate triesters. WO2007/070598 (herein “Spring Bank II”) teaches phosphotriester prodrugs as antiviral nucleic acids and teaches the synthesis of phosphorothioate prodrugs. Spring Bank I and II do not teach chirally controlled oligonucleotides, compositions thereof, and methods of making and using the same, as described by the present invention. 
     EP0779893 (herein “Hybridon”) teaches lipophilic prodrugs for the increased cellular uptake of antisense oligonucleotides and observes that Rp and Sp phosphorothioates and phosphorothioate triester dimers can have different enzymatic stability properties. Hybridon does not teach chirally controlled oligonucleotides, compositions thereof, and methods of making and using the same, as described by the present invention. 
     WO1997/047637 (herein “Imbach I”) teaches generally the Imbach “SATE” (S-acyl thioethyl) prodrug oligonucleotide compositions and methods. Imbach I describes, for example, bioreversible phosphotriester prodrugs and the preparation of certain prodrug oligonucleotides using post-synthetic alkylation or prodrug-group-containing phosphoramidites. U.S. Pat. No. 6,124,445 (herein “Imbach II”) teaches modified antisense and chimeric prodrug oligonucleotides. Imbach I and II do not teach chirally controlled oligonucleotides, compositions thereof, and methods of making and using the same, as described by the present invention. 
     WO2006/065751 (herein “Beaucage”) teaches CpG oligonucleotide phosphorothioate prodrugs that comprise thermolabile substituents (which substituents are introduced via a phosphoramidite monomer), and applications thereof. Beaucage does not teach chirally controlled oligonucleotides, compositions thereof, and methods of making and using the same, as described by the present invention. 
     Takeshi Wada et al. developed novel methods for the stereo-controlled synthesis of P-chiral nucleic acids using amidite chiral auxiliaries (JP4348077, WO2005/014609, WO2005/092909, and WO2010/064146, cumulatively referred to herein as “Wada I”). In particular, WO2010/064146 (referred to herein as “Wada II”) discloses methods for synthesizing phosphorus atom-modified nucleic acids wherein the stereochemical configuration at phosphorus is controlled. However, the methods of Wada II are limited in that they do not provide for individual P-modification of each chiral linkage phosphorus in a controlled and designed manner. That is, the methods for P-modified linkages of Wada II provide for the generation of a condensed intermediate poly H-phosphonate oligonucleotide strand that, once built to a desired length, is mass modified at the linkage phosphorus to provide, e.g., a desired phosphorothioate diester, phosphoramidate or boranophosphate or other such phosphorus atom-modified nucleic acids (referred to as Route B in the document—Scheme 6, page 36). Furthermore, the H-phosphonate oligonucleotide strands of Wada II are of shorter lengths (e.g., dimer trimer, or tetramer). Combined with the fact that there is no capping step in route B, which generally presents low crude purity as a result of the accumulation of “n−1”-type byproducts, the Wada II route contains limitations in regards of the synthesis of longer oligonucleotides. While Wada II contemplates generally that a particular oligonucleotide could be envisaged to contain different modifications at each linkage phosphorus, Wada II does not describe or suggest methods for controlled iterative installation of such modifications, as are described herein. To the extent that Wada II depicts a synthetic cycle that does not require an H-phosphonate intermediate oligonucleotide to be completely assembled prior to modification at the linkage phosphorus (therein referred to as Route A, page 35, Scheme 5, “Synthesis of a nucleic acid comprising a chiral X-phosphonate moiety of Formula 1 via Route A”), this general disclosure does not teach certain key steps that are required to install certain P-modifications, as provided by the present invention, and especially not with any degree of efficiency and versatility such that this cycle would be useful in the synthesis of chirally controlled P-modified oligonucleotides, and especially oligonucleotides of longer lengths. 
     At least one such inefficiency of Wada II is noted by Wada et al. in WO2012/039448 (herein “Wada III”). Wada III teaches novel chiral auxiliaries for use in Wada II methods to produce H-phosphonate oligonucleotides that, once built, can be subsequently modified to provide, inter alia, phosphorothioates and the like. Wada et al. observe in Wada III that the four types of chiral auxiliaries disclosed in Wada II formed strong bonds with phosphorus at the linkage phosphorus and thus did not allow for efficient removal. Wada III notes that removal of the Wada II chiral auxiliaries required harsh conditions, which conditions were prone to compromising the integrity of the product oligonucleotide. Wada III observes that this is especially problematic when synthesizing long chain oligonucleotides for at least the reason that as the degradation reaction(s) proceed, additional byproducts are generated that can further react with and degrade the product oligonucleotide. Wada III therefore provides chiral auxiliaries that can be more efficiently cleaved from the oligonucleotide under mild acidic conditions by way of an S N 1 mechanism releasing the H-phosphonate internucleotide linkage (route B), or under relatively mild basic conditions, by a β-elimation pathway. 
     One of skill in the chemical and synthetic arts will immediately appreciate the complexities associated with generating chirally controlled oligonucleotides such as those provided by the present invention. For instance, in order to synthesize and isolate a chirally controlled oligonucleotide, conditions for each monomer addition must be designed such that (1) the chemistry is compatible with every portion of the growing oligonucleotide; (2) the byproducts generated during each monomer addition do not compromise the structural and stereochemical integrity of the growing oligonucleotide; and (3) the crude final product composition is a composition which allows for isolation of the desired chirally controlled oligonucleotide product. 
     Oligonucleotide phosphorothioates have shown therapeutic potential (Stein et al., Science (1993), 261:1004-12; Agrawal et al., Antisence Res. and Dev. (1992), 2:261-66; Bayever et al., Antisense Res. and Dev. (1993), 3:383-390). Oligonucleotide phosphorothioates prepared without regard to the stereochemistry of the phosphorothioate exist as a mixture of 2 n  diastereomers, where n is the number of internucleotide phosphorothioates linkages. The chemical and biological properties of these diastereomeric phosphorothioates can be distinct. For example, Wada et al (Nucleic Acids Symposium Series No. 51 p. 119-120; doi:10.1093/nass/nrm060) found that stereodefined-(Rp)-(Ups)9U/(Ap)9A duplex showed a higher Tm value than that of natural-(Up)9U/(Ap)9A and stereodefined-(Sp)-(Ups)9U did not form a duplex. In another example, in a study by Tang et al., (Nucleosides Nucleotides (1995), 14:985-990) stereopure Rp-oligodeoxyribonucleoside phosphorothioates were found to possess lower stability to nucleases endogenous to human serum that the parent oligodeoxyribonucleoside phosphorothioates with undefined phosphorus chirality. 
     Chirally Controlled Oligonucleotides and Chirally Controlled Oligonucleotide Compositions 
     The present invention provides chirally controlled oligonucleotides, and chirally controlled oligonucleotide compositions which are of high crude purity and of high diastereomeric purity. In some embodiments, the present invention provides chirally controlled oligonucleotides, and chirally controlled oligonucleotide compositions which are of high crude purity. In some embodiments, the present invention provides chirally controlled oligonucleotides, and chirally controlled oligonucleotide compositions which are of high diastereomeric purity. 
     In some embodiments, the present invention provides chirally controlled compositions comprising a plurality of oligonucleotides of at least one type, wherein each type is defined by: 1) base sequence; 2) pattern of backbone linkages; 3) pattern of backbone chiral centers; and 4) pattern of backbone P-modifications. 
     In some embodiments, the present invention provides chirally controlled compositions comprising a plurality of oligonucleotides of the same type, wherein each type is defined by: 1) base sequence; 2) pattern of backbone linkages; 3) pattern of backbone chiral centers; and 4) pattern of backbone P-modifications. In some embodiments, the present invention provides chirally controlled compositions comprising a plurality of oligonucleotides of two or more types, wherein each type is defined by: 1) base sequence; 2) pattern of backbone linkages; 3) pattern of backbone chiral centers; and 4) pattern of backbone P-modifications. 
     In some embodiments, the present invention provides oligonucleotides comprising one or more diastereomerically pure internucleotidic linkages with respect to the chiral linkage phosphorus. In some embodiments, the present invention provides oligonucleotides comprising one or more diastereomerically pure internucleotidic linkages having the structure of formula I. In some embodiments, the present invention provides oligonucleotides comprising one or more diastereomerically pure internucleotidic linkages with respect to the chiral linkage phosphorus, and one or more phosphate diester linkages. In some embodiments, the present invention provides oligonucleotides comprising one or more diastereomerically pure internucleotidic linkages having the structure of formula I, and one or more phosphate diester linkages. In some embodiments, the present invention provides oligonucleotides comprising one or more diastereomerically pure internucleotidic linkages having the structure of formula I-c, and one or more phosphate diester linkages. In some embodiments, such oligonucleotides are prepared by using stereoselective oligonucleotide synthesis, as described in this application, to form pre-designed diastereomerically pure internucleotidic linkages with respect to the chiral linkage phosphorus. For instance, in one exemplary oligonucleotide of (Rp/Sp, Rp/Sp, Rp/Sp, Rp, Rp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGs1Cs1As1CsC] (SEQ ID NO: 106), the first three internucleotidic linkages are constructed using traditional oligonucleotide synthesis method, and the diastereomerically pure internucleotidic linkages are constructed with stereochemical control as described in this application. Exemplary internucleotidic linkages, including those having structures of formula I, are further described below. In some embodiments, such oligonucleotides comprise a sequence further described in the application, including but not limited to those described in Tables 2 and 4, and Appendices A, B and C. 
     In some embodiments, a provided oligonucleotide comprises a combination of stereopure and stereorandom internucleotidic linkages with respect to chirality at the linkage phosphorus. For instance, in some embodiments it is desirable to have a block of one or more stereodefined internucleotidic linkages within an oligonucleotide that is otherwise stereorandom with respect to chirality at the linkage phosphorus. In some embodiments, it is desirable to have a block of one or more internucleotidic linkages that are stereorandom within an oligonucleotide that is otherwise stereodefined with respect to chirality at the linkage phosphorus. 
     In some embodiments, at least one nucleotide unit of a provided oligonucleotide is installed using stereoselective oligonucleotide synthesis, as described in this application, to form a pre-designed diastereomerically pure internucleotidic linkage with respect to the chiral linkage phosphorus. In some embodiments, at least two nucleotide units of a provided oligonucleotide are installed using stereoselective oligonucleotide synthesis, as described in this application, to form at least two pre-designed diastereomerically pure internucleotidic linkages with respect to the chiral linkage phosphorus. In some embodiments, at least three nucleotide units of a provided oligonucleotide are installed using stereoselective oligonucleotide synthesis, as described in this application, to form at least three pre-designed diastereomerically pure internucleotidic linkages with respect to the chiral linkage phosphorus. In some embodiments, the at least one, two, or three pre-designed diastereomerically pure internucleotidic linkages are adjacent to one another. In some embodiments, the at least one, two, or three pre-designed diastereomerically pure internucleotidic linkages are not adjacent to one another. 
     In some embodiments, at least about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, or 50% of nucleotide units of a provided oligonucleotide are installed using stereoselective oligonucleotide synthesis, as described in this application, to form a pre-designed diastereomerically pure internucleotidic linkage with respect to the chiral linkage phosphorus. As described herein, in some embodiments the at least about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, or 50% of nucleotide units occur in one or more blocks to provide a blockmer. In some embodiments, the at least about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, or 50% of nucleotide units occur in a an alternating pattern to provide an altmer. One of skill in the relevant arts will recognize that any desirable pattern can be achieved using methods of the present invention and are contemplated herein. 
     In some embodiments, the present invention provides a chirally controlled oligonucleotide, wherein at least two of the individual internucleotidic linkages within the oligonucleotide have different stereochemistry and/or different P-modifications relative to one another. In certain embodiments, the present invention provides a chirally controlled oligonucleotide, wherein at least two individual internucleotidic linkages within the oligonucleotide have different P-modifications relative to one another. In certain embodiments, the present invention provides a chirally controlled oligonucleotide, wherein at least two of the individual internucleotidic linkages within the oligonucleotide have different P-modifications relative to one another, and wherein the chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage. In certain embodiments, the present invention provides a chirally controlled oligonucleotide, wherein at least two of the individual internucleotidic linkages within the oligonucleotide have different P-modifications relative to one another, and wherein the chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage and at least one phosphorothioate diester internucleotidic linkage. In certain embodiments, the present invention provides a chirally controlled oligonucleotide, wherein at least two of the individual internucleotidic linkages within the oligonucleotide have different P-modifications relative to one another, and wherein the chirally controlled oligonucleotide comprises at least one phosphorothioate triester internucleotidic linkage. In certain embodiments, the present invention provides a chirally controlled oligonucleotide, wherein at least two of the individual internucleotidic linkages within the oligonucleotide have different P-modifications relative to one another, and wherein the chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage and at least one phosphorothioate triester internucleotidic linkage. 
     In certain embodiments, the present invention provides a chirally controlled oligonucleotide comprising one or more modified internucleotidic linkages independently having the structure of formula I: 
                         
wherein each variable is as defined and described below. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising one or more modified internucleotidic linkages of formula I, and wherein individual internucleotidic linkages of formula I within the oligonucleotide have different P-modifications relative to one another. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising one or more modified internucleotidic linkages of formula I, and wherein individual internucleotidic linkages of formula I within the oligonucleotide have different —X-L-R 1  relative to one another. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising one or more modified internucleotidic linkages of formula I and wherein individual internucleotidic linkages of formula I within the oligonucleotide have different X relative to one another. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising one or more modified internucleotidic linkages of formula I, and wherein individual internucleotidic linkages of formula I within the oligonucleotide have different -L-R 1  relative to one another.
 
     In some embodiments, the present invention provides a chirally controlled oligonucleotide, wherein at least two of the individual internucleotidic linkages within the oligonucleotide have different stereochemistry and/or different P-modifications relative to one another. In some embodiments, the present invention provides a chirally controlled oligonucleotide, wherein at least two of the individual internucleotidic linkages within the oligonucleotide have different stereochemistry relative to one another, and wherein at least a portion of the structure of the chirally controlled oligonucleotide is characterized by a repeating pattern of alternating stereochemistry. 
     In some embodiments, the present invention provides a chirally controlled oligonucleotide, wherein at least two of the individual internucleotidic linkages within the oligonucleotide have different P-modifications relative to one another, in that they have different X atoms in their —XLR 1  moieties, and/or in that they have different L groups in their —XLR 1  moieties, and/or that they have different R 1  atoms in their —XLR 1  moieties. 
     In some embodiments, the present invention provides a chirally controlled oligonucleotide, wherein at least two of the individual internucleotidic linkages within the oligonucleotide have different stereochemistry and/or different P-modifications relative to one another and the oligonucleotide has a structure represented by the following formula:
 
[S B n1R B n2S B n3R B n4 . . . S B nxR B ny]
 
wherein:
 
each R B  independently represents a block of nucleotide units having the R configuration at the linkage phosphorus;
 
each S B  independently represents a block of nucleotide units having the S configuration at the linkage phosphorus;
 
each of n1-ny is zero or an integer, with the requirement that at least one odd n and at least one even n must be non-zero so that the oligonucleotide includes at least two individual internucleotidic linkages with different stereochemistry relative to one another; and
 
wherein the sum of n1-ny is between 2 and 200, and in some embodiments is between a lower limit selected from the group consisting of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or more and an upper limit selected from the group consisting of 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, and 200, the upper limit being larger than the lower limit.
 
     In some such embodiments, each n has the same value; in some embodiments, each even n has the same value as each other even n; in some embodiments, each odd n has the same value each other odd n; in some embodiments, at least two even ns have different values from one another; in some embodiments, at least two odd ns have different values from one another. 
     In some embodiments, at least two adjacent ns are equal to one another, so that a provided oligonucleotide includes adjacent blocks of S stereochemistry linkages and R stereochemistry linkages of equal lengths. In some embodiments, provided oligonucleotides include repeating blocks of S and R stereochemistry linkages of equal lengths. In some embodiments, provided oligonucleotides include repeating blocks of S and R stereochemistry linkages, where at least two such blocks are of different lengths from one another; in some such embodiments each S stereochemistry block is of the same length, and is of a different length from each R stereochemistry length, which may optionally be of the same length as one another. 
     In some embodiments, at least two skip-adjacent ns are equal to one another, so that a provided oligonucleotide includes at least two blocks of linkages of a first stereochemistry that are equal in length to one another and are separated by a block of linkages of the other stereochemistry, which separating block may be of the same length or a different length from the blocks of first steroechemistry. 
     In some embodiments, ns associated with linkage blocks at the ends of a provided oligonucleotide are of the same length. In some embodiments, provided oligonucleotides have terminal blocks of the same linkage stereochemistry. In some such embodiments, the terminal blocks are separated from one another by a middle block of the other linkage stereochemistry. 
     In some embodiments, a provided oligonucleotide of formula [S B n1R B n2S B n3R B n4 . . . S B nxR B ny] is a stereoblockmer. In some embodiments, a provided oligonucleotide of formula [S B n1R B n2S B n3R B n4 . . . S B nxR B ny] is a stereoskipmer. In some embodiments, a provided oligonucleotide of formula [S B n1R B n2S B n3R B n4 . . . S B nxR B ny] is a stereoaltmer. In some embodiments, a provided oligonucleotide of formula [S B n1R B n2S B n3R B n4 . . . S B nxR B ny] is a gapmer. 
     In some embodiments, a provided oligonucleotide of formula [S B n1R B n2S B n3R B n4 . . . S B nxR B ny] is of any of the above described patterns and further comprises patterns of P-modifications. For instance, in some embodiments, a provided oligonucleotide of formula [S B n1R B n2S B n3R B n4 . . . S B nxR B ny] and is a stereoskipmer and P-modification skipmer. In some embodiments, a provided oligonucleotide of formula [S B n1R B n2S B n3R B n4 . . . S B nxR B ny] and is a stereoblockmer and P-modification altmer. In some embodiments, a provided oligonucleotide of formula [S B n1R B n2S B n3R B n4 . . . S B nxR B ny] and is a stereoaltmer and P-modification blockmer. 
     In some embodiments, a provided oligonucleotide of formula [S B n1R B n2S B n3R B n4 . . . S B nxR B ny] is a chirally controlled oligonucleotide comprising one or more modified internucleotidic linkages independently having the structure of formula I: 
                         
wherein:
     P* is an asymmetric phosphorus atom and is either Rp or Sp;   W is O, S or Se;   each of X, Y and Z is independently —O—, —S—, —N(-L-R 1 )—, or L;   L is a covalent bond or an optionally substituted, linear or branched C 1 -C 10  alkylene, wherein one or more methylene units of L are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—;   R 1  is halogen, R, or an optionally substituted C 1 -C 50  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—;   each R′ is independently —R, —C(O)R, —CO 2 R, or —SO 2 R, or:
       two R′ on the same nitrogen are taken together with their intervening atoms to form an optionally substituted heterocyclic or heteroaryl ring, or   two R′ on the same carbon are taken together with their intervening atoms to form an optionally substituted aryl, carbocyclic, heterocyclic, or heteroaryl ring;   
       -Cy- is an optionally substituted bivalent ring selected from phenylene, carbocyclylene, arylene, heteroarylene, or heterocyclylene;   each R is independently hydrogen, or an optionally substituted group selected from C 1 -C 6  aliphatic, phenyl, carbocyclyl, aryl, heteroaryl, or heterocyclyl; and   each   

                         
independently represents a connection to a nucleoside.
 
     In some embodiments, a chirally controlled oligonucleotide comprises one or more modified internucleotidic phosphorus linkages. In some embodiments, a chirally controlled oligonucleotide comprises, e.g., a phosphorothioate or a phosphorothioate triester linkage. In some embodiments, a chirally controlled oligonucleotide comprises a phosphorothioate triester linkage. In some embodiments, a chirally controlled oligonucleotide comprises at least two phosphorothioate triester linkages. In some embodiments, a chirally controlled oligonucleotide comprises at least three phosphorothioate triester linkages. In some embodiments, a chirally controlled oligonucleotide comprises at least four phosphorothioate triester linkages. In some embodiments, a chirally controlled oligonucleotide comprises at least five phosphorothioate triester linkages. Exemplary such modified internucleotidic phosphorus linkages are described further herein. 
     In some embodiments, a chirally controlled oligonucleotide comprises different internucleotidic phosphorus linkages. In some embodiments, a chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage and at least one modified internucleotidic linkage. In some embodiments, a chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage and at least one phosphorothioate triester linkage. In some embodiments, a chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage and at least two phosphorothioate triester linkages. In some embodiments, a chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage and at least three phosphorothioate triester linkages. In some embodiments, a chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage and at least four phosphorothioate triester linkages. In some embodiments, a chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage and at least five phosphorothioate triester linkages. Exemplary such modified internucleotidic phosphorus linkages are described further herein. 
     In some embodiments, a phosphorothioate triester linkage comprises a chiral auxiliary, which, for example, is used to control the stereoselectivity of a reaction. In some embodiments, a phosphorothioate triester linkage does not comprise a chiral auxiliary. In some embodiments, a phosphorothioate triester linkage is intentionally maintained until and/or during the administration to a subject. 
     In some embodiments, a chirally controlled oligonucleotide is linked to a solid support. In some embodiments, a chirally controlled oligonucleotide is cleaved from a solid support. 
     In some embodiments, a chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage and at least two consecutive modified internucleotidic linkages. In some embodiments, a chirally controlled oligonucleotide comprises at least one phosphate diester internucleotidic linkage and at least two consecutive phosphorothioate triester internucleotidic linkages. 
     In some embodiments, a chirally controlled oligonucleotide is a blockmer. In some embodiments, a chirally controlled oligonucleotide is a stereoblockmer. In some embodiments, a chirally controlled oligonucleotide is a P-modification blockmer. In some embodiments, a chirally controlled oligonucleotide is a linkage blockmer. 
     In some embodiments, a chirally controlled oligonucleotide is an altmer. In some embodiments, a chirally controlled oligonucleotide is a stereoaltmer. In some embodiments, a chirally controlled oligonucleotide is a P-modification altmer. In some embodiments, a chirally controlled oligonucleotide is a linkage altmer. 
     In some embodiments, a chirally controlled oligonucleotide is a unimer. In some embodiments, a chirally controlled oligonucleotide is a stereounimer. In some embodiments, a chirally controlled oligonucleotide is a P-modification unimer. In some embodiments, a chirally controlled oligonucleotide is a linkage unimer. 
     In some embodiments, a chirally controlled oligonucleotide is a gapmer. 
     In some embodiments, a chirally controlled oligonucleotide is a skipmer. 
     In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising one or more modified internucleotidic linkages independently having the structure of formula I: 
                         
wherein:
     P* is an asymmetric phosphorus atom and is either Rp or Sp;   W is O, S or Se;   each of X, Y and Z is independently —O—, —S—, —N(-L-R 1 )—, or L;   L is a covalent bond or an optionally substituted, linear or branched C 1 -C 10  alkylene, wherein one or more methylene units of L are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—;   R 1  is halogen, R, or an optionally substituted C 1 -C 50  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—;   each R′ is independently —R, —C(O)R, —CO 2 R, or —SO 2 R, or:
       two R′ on the same nitrogen are taken together with their intervening atoms to form an optionally substituted heterocyclic or heteroaryl ring, or   two R′ on the same carbon are taken together with their intervening atoms to form an optionally substituted aryl, carbocyclic, heterocyclic, or heteroaryl ring;   
       -Cy- is an optionally substituted bivalent ring selected from phenylene, carbocyclylene, arylene, heteroarylene, or heterocyclylene;   each R is independently hydrogen, or an optionally substituted group selected from C 1 -C 6  aliphatic, phenyl, carbocyclyl, aryl, heteroaryl, or heterocyclyl; and   each   independently represents a connection to a nucleoside.   

     As defined generally above and herein, P* is an asymmetric phosphorus atom and is either Rp or Sp. In some embodiments, P* is Rp. In other embodiments, P* is Sp. In some embodiments, an oligonucleotide comprises one or more internucleotidic linkages of formula I wherein each P* is independently Rp or Sp. In some embodiments, an oligonucleotide comprises one or more internucleotidic linkages of formula I wherein each P* is Rp. In some embodiments, an oligonucleotide comprises one or more internucleotidic linkages of formula I wherein each P* is Sp. In some embodiments, an oligonucleotide comprises at least one internucleotidic linkage of formula I wherein P* is Rp. In some embodiments, an oligonucleotide comprises at least one internucleotidic linkage of formula I wherein P* is Sp. In some embodiments, an oligonucleotide comprises at least one internucleotidic linkage of formula I wherein P* is Rp, and at least one internucleotidic linkage of formula I wherein P* is Sp. 
     As defined generally above and herein, W is O, S, or Se. In some embodiments, W is O. In some embodiments, W is S. In some embodiments, W is Se. In some embodiments, an oligonucleotide comprises at least one internucleotidic linkage of formula I wherein W is O. In some embodiments, an oligonucleotide comprises at least one internucleotidic linkage of formula I wherein W is S. In some embodiments, an oligonucleotide comprises at least one internucleotidic linkage of formula I wherein W is Se. 
     As defined generally above and herein, each R is independently hydrogen, or an optionally substituted group selected from C 1 -C 6  aliphatic, phenyl, carbocyclyl, aryl, heteroaryl, or heterocyclyl. 
     In some embodiments, R is hydrogen. In some embodiments, R is an optionally substituted group selected from C 1 -C 6  aliphatic, phenyl, carbocyclyl, aryl, heteroaryl, or heterocyclyl. 
     In some embodiments, R is an optionally substituted C 1 -C 6  aliphatic. In some embodiments, R is an optionally substituted C 1 -C 6  alkyl. In some embodiments, R is optionally substituted, linear or branched hexyl. In some embodiments, R is optionally substituted, linear or branched pentyl. In some embodiments, R is optionally substituted, linear or branched butyl. In some embodiments, R is optionally substituted, linear or branched propyl. In some embodiments, R is optionally substituted ethyl. In some embodiments, R is optionally substituted methyl. 
     In some embodiments, R is optionally substituted phenyl. In some embodiments, R is substituted phenyl. In some embodiments, R is phenyl. 
     In some embodiments, R is optionally substituted carbocyclyl. In some embodiments, R is optionally substituted C 3 -C 10  carbocyclyl. In some embodiments, R is optionally substituted monocyclic carbocyclyl. In some embodiments, R is optionally substituted cycloheptyl. In some embodiments, R is optionally substituted cyclohexyl. In some embodiments, R is optionally substituted cyclopentyl. In some embodiments, R is optionally substituted cyclobutyl. In some embodiments, R is an optionally substituted cyclopropyl. In some embodiments, R is optionally substituted bicyclic carbocyclyl. 
     In some embodiments, R is an optionally substituted aryl. In some embodiments, R is an optionally substituted bicyclic aryl ring. 
     In some embodiments, R is an optionally substituted heteroaryl. In some embodiments, R is an optionally substituted 5-6 membered monocyclic heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, sulfur, or oxygen. In some embodiments, R is a substituted 5-6 membered monocyclic heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an unsubstituted 5-6 membered monocyclic heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, sulfur, or oxygen. 
     In some embodiments, R is an optionally substituted 5 membered monocyclic heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, oxygen or sulfur. In some embodiments, R is an optionally substituted 6 membered monocyclic heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     In some embodiments, R is an optionally substituted 5-membered monocyclic heteroaryl ring having 1 heteroatom selected from nitrogen, oxygen, or sulfur. In some embodiments, R is selected from pyrrolyl, furanyl, or thienyl. 
     In some embodiments, R is an optionally substituted 5-membered heteroaryl ring having 2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In certain embodiments, R is an optionally substituted 5-membered heteroaryl ring having 1 nitrogen atom, and an additional heteroatom selected from sulfur or oxygen. Exemplary R groups include optionally substituted pyrazolyl, imidazolyl, thiazolyl, isothiazolyl, oxazolyl or isoxazolyl. 
     In some embodiments, R is a 6-membered heteroaryl ring having 1-3 nitrogen atoms. In other embodiments, R is an optionally substituted 6-membered heteroaryl ring having 1-2 nitrogen atoms. In some embodiments, R is an optionally substituted 6-membered heteroaryl ring having 2 nitrogen atoms. In certain embodiments, R is an optionally substituted 6-membered heteroaryl ring having 1 nitrogen. Exemplary R groups include optionally substituted pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazinyl, or tetrazinyl. 
     In certain embodiments, R is an optionally substituted 8-10 membered bicyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an optionally substituted 5,6-fused heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In other embodiments, R is an optionally substituted 5,6-fused heteroaryl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In certain embodiments, R is an optionally substituted 5,6-fused heteroaryl ring having 1 heteroatom independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an optionally substituted indolyl. In some embodiments, R is an optionally substituted azabicyclo[3.2.1]octanyl. In certain embodiments, R is an optionally substituted 5,6-fused heteroaryl ring having 2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an optionally substituted azaindolyl. In some embodiments, R is an optionally substituted benzimidazolyl. In some embodiments, R is an optionally substituted benzothiazolyl. In some embodiments, R is an optionally substituted benzoxazolyl. In some embodiments, R is an optionally substituted indazolyl. In certain embodiments, R is an optionally substituted 5,6-fused heteroaryl ring having 3 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     In certain embodiments, R is an optionally substituted 6,6-fused heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an optionally substituted 6,6-fused heteroaryl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In other embodiments, R is an optionally substituted 6,6-fused heteroaryl ring having 1 heteroatom independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an optionally substituted quinolinyl. In some embodiments, R is an optionally substituted isoquinolinyl. According to one aspect, R is an optionally substituted 6,6-fused heteroaryl ring having 2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is a quinazoline or a quinoxaline. 
     In some embodiments, R is an optionally substituted heterocyclyl. In some embodiments, R is an optionally substituted 3-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is a substituted 3-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an unsubstituted 3-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     In some embodiments, R is an optionally substituted heterocyclyl. In some embodiments, R is an optionally substituted 6 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an optionally substituted 6 membered partially unsaturated heterocyclic ring having 2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an optionally substituted 6 membered partially unsaturated heterocyclic ring having 2 oxygen atom. 
     In certain embodiments, R is a 3-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In certain embodiments, R is oxiranyl, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, oxepaneyl, aziridineyl, azetidineyl, pyrrolidinyl, piperidinyl, azepanyl, thiiranyl, thietanyl, tetrahydrothiophenyl, tetrahydrothiopyranyl, thiepanyl, dioxolanyl, oxathiolanyl, oxazolidinyl, imidazolidinyl, thiazolidinyl, dithiolanyl, dioxanyl, morpholinyl, oxathianyl, piperazinyl, thiomorpholinyl, dithianyl, dioxepanyl, oxazepanyl, oxathiepanyl, dithiepanyl, diazepanyl, dihydrofuranonyl, tetrahydropyranonyl, oxepanonyl, pyrolidinonyl, piperidinonyl, azepanonyl, dihydrothiophenonyl, tetrahydrothiopyranonyl, thiepanonyl, oxazolidinonyl, oxazinanonyl, oxazepanonyl, dioxolanonyl, dioxanonyl, dioxepanonyl, oxathiolinonyl, oxathianonyl, oxathiepanonyl, thiazolidinonyl, thiazinanonyl, thiazepanonyl, imidazolidinonyl, tetrahydropyrimidinonyl, diazepanonyl, imidazolidinedionyl, oxazolidinedionyl, thiazolidinedionyl, dioxolanedionyl, oxathiolanedionyl, piperazinedionyl, morpholinedionyl, thiomorpholinedionyl, tetrahydropyranyl, tetrahydrofuranyl, morpholinyl, thiomorpholinyl, piperidinyl, piperazinyl, pyrrolidinyl, tetrahydrothiophenyl, or tetrahydrothiopyranyl. In some embodiments, R is an optionally substituted 5 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     In certain embodiments, R is an optionally substituted 5-6 membered partially unsaturated monocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In certain embodiments, R is an optionally substituted tetrahydropyridinyl, dihydrothiazolyl, dihydrooxazolyl, or oxazolinyl group. 
     In some embodiments, R is an optionally substituted 8-10 membered bicyclic saturated or partially unsaturated heterocyclic ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an optionally substituted indolinyl. In some embodiments, R is an optionally substituted isoindolinyl. In some embodiments, R is an optionally substituted 1, 2, 3, 4-tetrahydroquinoline. In some embodiments, R is an optionally substituted 1, 2, 3, 4-tetrahydroisoquinoline. 
     As defined generally above and herein, each R′ is independently —R, —C(O)R, —CO 2 R, or —SO 2 R, or:
         two R′ on the same nitrogen are taken together with their intervening atoms to form an optionally substituted heterocyclic or heteroaryl ring, or   two R′ on the same carbon are taken together with their intervening atoms to form an optionally substituted aryl, carbocyclic, heterocyclic, or heteroaryl ring.       

     In some embodiments, R′ is —R, —C(O)R, —CO 2 R, or —SO 2 R, wherein R is as defined above and described herein. 
     In some embodiments, R′ is —R, wherein R is as defined and described above and herein. In some embodiments, R′ is hydrogen. 
     In some embodiments, R′ is —C(O)R, wherein R is as defined above and described herein. In some embodiments, R′ is —CO 2 R, wherein R is as defined above and described herein. In some embodiments, R′ is —SO 2 R, wherein R is as defined above and described herein. 
     In some embodiments, two R′ on the same nitrogen are taken together with their intervening atoms to form an optionally substituted heterocyclic or heteroaryl ring. In some embodiments, two R′ on the same carbon are taken together with their intervening atoms to form an optionally substituted aryl, carbocyclic, heterocyclic, or heteroaryl ring. 
     As defined generally above and herein, -Cy- is an optionally substituted bivalent ring selected from phenylene, carbocyclylene, arylene, heteroarylene, or heterocyclylene. 
     In some embodiments, -Cy- is optionally substituted phenylene. In some embodiments, -Cy- is optionally substituted carbocyclylene. In some embodiments, -Cy- is optionally substituted arylene. In some embodiments, -Cy- is optionally substituted heteroarylene. In some embodiments, -Cy- is optionally substituted heterocyclylene. 
     As defined generally above and herein, each of X, Y and Z is independently —O—, —S—, —N(-L-R 1 )—, or L, wherein each of L and R 1  is independently as defined above and described below. 
     In some embodiments, X is —O—. In some embodiments, X is —S—. In some embodiments, X is —O— or —S—. In some embodiments, an oligonucleotide comprises at least one internucleotidic linkage of formula I wherein X is —O—. In some embodiments, an oligonucleotide comprises at least one internucleotidic linkage of formula I wherein X is —S—. In some embodiments, an oligonucleotide comprises at least one internucleotidic linkage of formula I wherein X is —O—, and at least one internucleotidic linkage of formula I wherein X is —S—. In some embodiments, an oligonucleotide comprises at least one internucleotidic linkage of formula I wherein X is —O—, and at least one internucleotidic linkage of formula I wherein X is —S—, and at least one internucleotidic linkage of formula I wherein L is an optionally substituted, linear or branched C 1 -C 10  alkylene, wherein one or more methylene units of L are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—. 
     In some embodiments, X is —N(-L-R 1 )—. In some embodiments, X is —N(R 1 )—. In some embodiments, X is —N(R′)—. In some embodiments, X is —N(R)—. In some embodiments, X is —NH—. 
     In some embodiments, X is L. In some embodiments, X is a covalent bond. In some embodiments, X is or an optionally substituted, linear or branched C 1 -C 10  alkylene, wherein one or more methylene units of L are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—. In some embodiments, X is an optionally substituted C 1 -C 10  alkylene or C 1 -C 10  alkenylene. In some embodiments, X is methylene. 
     In some embodiments, Y is —O—. In some embodiments, Y is —S—. 
     In some embodiments, Y is —N(-L-R 1 )—. In some embodiments, Y is —N(R 1 )—. In some embodiments, Y is —N(R′)—. In some embodiments, Y is —N(R)—. In some embodiments, Y is —NH—. 
     In some embodiments, Y is L. In some embodiments, Y is a covalent bond. In some embodiments, Y is or an optionally substituted, linear or branched C 1 -C 10  alkylene, wherein one or more methylene units of L are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—. In some embodiments, Y is an optionally substituted C 1 -C 10  alkylene or C 1 -C 10  alkenylene. In some embodiments, Y is methylene. 
     In some embodiments, Z is —O—. In some embodiments, Z is —S—. 
     In some embodiments, Z is —N(-L-R 1 )—. In some embodiments, Z is —N(R 1 )—. In some embodiments, Z is —N(R′)—. In some embodiments, Z is —N(R)—. In some embodiments, Z is —NH—. 
     In some embodiments, Z is L. In some embodiments, Z is a covalent bond. In some embodiments, Z is or an optionally substituted, linear or branched C 1 -C 10  alkylene, wherein one or more methylene units of L are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—. In some embodiments, Z is an optionally substituted C 1 -C 10  alkylene or C 1 -C 10  alkenylene. In some embodiments, Z is methylene. 
     As defined generally above and herein, L is a covalent bond or an optionally substituted, linear or branched C 1 -C 10  alkylene, wherein one or more methylene units of L are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—. 
     In some embodiments, L is a covalent bond. In some embodiments, L is an optionally substituted, linear or branched C 1 -C 10  alkylene, wherein one or more methylene units of L are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—. 
     In some embodiments, L has the structure of -L 1 -V—, wherein: 
     L 1  is an optionally substituted group selected from 
                         
C 1 -C 6  alkylene, C 1 -C 6  alkenylene, carbocyclylene, arylene, C 1 -C 6  heteroalkylene, heterocyclylene, and heteroarylene;
 
V is selected from —O—, —S—, —NR′—, C(R′) 2 , —S—S—, —B—S—S—C—,
 
                         
or an optionally substituted group selected from C 1 -C 6  alkylene, arylene, C 1 -C 6  heteroalkylene, heterocyclylene, and heteroarylene;
 
A is ═O, ═S, ═NR′, or ═C(R′) 2 ;
 
each of B and C is independently —O—, —S—, —NR′—, —C(R′) 2 —, or an optionally substituted group selected from C 1 -C 6  alkylene, carbocyclylene, arylene, heterocyclylene, or heteroarylene; and
 
each R′ is independently as defined above and described herein.
 
     In some embodiments, L 1  is 
     
       
         
         
             
             
         
       
     
     In some embodiments, L 1  is 
                         
wherein Ring Cy′ is an optionally substituted arylene, carbocyclylene, heteroarylene, or heterocyclylene. In some embodiments, L 1  is optionally substituted
 
                         
In some embodiments, L 1  is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, L 1  is connected to X. In some embodiments, L 1  is an optionally substituted group selected from 
                         
and the sulfur atom is connect to V. In some embodiments, L 1  is an optionally substituted group selected from
 
                         
and the carbon atom is connect to X.
 
     In some embodiments, L has the structure of: 
                         
wherein:
 
E is —O—, —S—, —NR′— or —C(R′) 2 —;
 
  is a single or double bond;
 
the two R L1  are taken together with the two carbon atoms to which they are bound to form an optionally substituted aryl, carbocyclic, heteroaryl or heterocyclic ring; and each R′ is independently as defined above and described herein.
 
     In some embodiments, L has the structure of: 
                         
wherein:
     G is —O—, —S—, or —NR′;      is a single or double bond; and
 
the two R L1  are taken together with the two carbon atoms to which they are bound to form an optionally substituted aryl, C 3 -C 10  carbocyclic, heteroaryl or heterocyclic ring.
   

     In some embodiments, L has the structure of: 
                         
wherein:
     E is —O—, —S—, —NR′— or —C(R′) 2 —;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—; and   each R′ is independently as defined above and described herein.   

     In some embodiments, L has the structure of: 
                         
wherein:
     G is —O—, —S—, or —NR′;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))—, or ═C(CF 3 )—.   

     In some embodiments, L has the structure of: 
                         
wherein:
     E is —O—, —S—, —NR′— or —C(R′) 2 —;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—; and   each R′ is independently as defined above and described herein.   

     In some embodiments, L has the structure of: 
                         
wherein:
     G is —O—, —S—, or —NR′;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—.   

     In some embodiments, L has the structure of: 
                         
wherein:
     E is —O—, —S—, —NR′— or —C(R′) 2 —;      is a single or double bond;
 
the two R L1  are taken together with the two carbon atoms to which they are bound to form an optionally substituted aryl, C 3 -C 10  carbocyclic, heteroaryl or heterocyclic ring; and each R′ is independently as defined above and described herein.
   

     In some embodiments, L has the structure of: 
                         
wherein:
     G is —O—, —S—, or —NR′;      is a single or double bond;
 
the two R L1  are taken together with the two carbon atoms to which they are bound to form an optionally substituted aryl, C 3 -C 10  carbocyclic, heteroaryl or heterocyclic ring; and each R′ is independently as defined above and described herein.
   

     In some embodiments, L has the structure of: 
                         
wherein:
     E is —O—, —S—, —NR′— or —C(R′) 2 —;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—; and   each R′ is independently as defined above and described herein.   

     In some embodiments, L has the structure of: 
                         
wherein:
     G is —O—, —S—, or —NR′;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—; and   each R′ is independently as defined above and described herein.   

     In some embodiments, L has the structure of: 
                         
wherein:
     E is —O—, —S—, —NR′— or —C(R′) 2 —;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—; and   each R′ is independently as defined above and described herein.   

     In some embodiments, L has the structure of: 
                         
wherein:
     G is —O—, —S—, or —NR′;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—; and   each R′ is independently as defined above and described herein.   

     In some embodiments, L has the structure of: 
                         
wherein:
     E is —O—, —S—, —NR′— or —C(R′) 2 —;      is a single or double bond;
 
the two R L1  are taken together with the two carbon atoms to which they are bound to form an optionally substituted aryl, C 3 -C 10  carbocyclic, heteroaryl or heterocyclic ring; and each R′ is independently as defined above and described herein.
   

     In some embodiments, L has the structure of: 
                         
wherein:
     G is —O—, —S—, or —NR′;      is a single or double bond;
 
the two R L1  are taken together with the two carbon atoms to which they are bound to form an optionally substituted aryl, C 3 -C 10  carbocyclic, heteroaryl or heterocyclic ring; and each R′ is independently as defined above and described herein.
   

     In some embodiments, L has the structure of: 
                         
wherein:
     E is —O—, —S—, —NR′— or —C(R′) 2 —;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—; and   each R′ is independently as defined above and described herein.   

     In some embodiments L has the structure f: 
                         
wherein:
     G is —O—, —S—, or —NR′;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—; and   R′ is as defined above and described herein.   

     In some embodiments, L has the structure of: 
                         
wherein:
     E is —O—, —S—, —NR′— or —C(R′) 2 —;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—; and   each R′ is independently as defined above and described herein.   

     In some embodiments, L has the structure of: 
                         
wherein:
     G is —O—, —S—, or —NR′;   D is ═N—, ═C(F)—, ═C(Cl)—, ═C(Br)—, ═C(I)—, ═C(CN)—, ═C(NO 2 )—, ═C(CO 2 —(C 1 -C 6  aliphatic))-, or ═C(CF 3 )—; and   R′ is as defined above and described herein.   

     In some embodiments, L has the structure of: 
                         
wherein the phenyl ring is optionally substituted. In some embodiments, the phenyl ring is not substituted. In some embodiments, the phenyl ring is substituted.
 
     In some embodiments, L has the structure of: 
                         
wherein the phenyl ring is optionally substituted. In some embodiments, the phenyl ring is not substituted. In some embodiments, the phenyl ring is substituted.
 
     In some embodiments, L has the structure of: 
                         
wherein:
        is a single or double bond; and   the two R L1  are taken together with the two carbon atoms to which they are bound to form an optionally substituted aryl, C 3 -C 10  carbocyclic, heteroaryl or heterocyclic ring.   

     In some embodiments, L has the structure of: 
                         
wherein:
     G is —O—, —S—, or —NR′;      is a single or double bond; and   the two R L1  are taken together with the two carbon atoms to which they are bound to form an optionally substituted aryl, C 3 -C 10  carbocyclic, heteroaryl or heterocyclic ring.   

     As defined generally above and herein, E is —O—, —S—, —NR′— or —C(R′) 2 —, wherein each R′ independently as defined above and described herein. In some embodiments, E is —O—, —S—, or —NR′—. In some embodiments, E is —O—, —S—, or —NH—. In some embodiments, E is —O—. In some embodiments, E is —S—. In some embodiments, E is —NH—. 
     As defined generally above and herein, G is —O—, —S—, or —NR′, wherein each R′ independently as defined above and described herein. In some embodiments, G is —O—, —S—, or —NH—. In some embodiments, G is —O—. In some embodiments, G is —S—. In some embodiments, G is —NH—. 
     In some embodiments, L is -L 3 -G-, wherein:
     L 3  is an optionally substituted C 1 -C 5  alkylene or alkenylene, wherein one or more methylene units are optionally and independently replaced by —O—, —S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —S(O)—, —S(O) 2 —, or   

                         
and
 
wherein each of G, R′ and Ring Cy′ is independently as defined above and described herein.
 
     In some embodiments, L is -L 3 -S—, wherein L 3  is as defined above and described herein. In some embodiments, L is -L 3 -O—, wherein L 3  is as defined above and described herein. In some embodiments, L is -L 3 -N(R′)—, wherein each of L 3  and R′ is independently as defined above and described herein. In some embodiments, L is -L 3 -NH—, wherein each of L 3  and R′ is independently as defined above and described herein. 
     In some embodiments, L 3  is an optionally substituted C 5  alkylene or alkenylene, wherein one or more methylene units are optionally and independently replaced by —O—, —S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —S(O)—, —S(O) 2 —, or 
                         
and each of R′ and Ring Cy′ is independently as defined above and described herein. In some embodiments, L 3  is an optionally substituted C 5  alkylene. In some embodiments, -L 3 -G- is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, L 3  is an optionally substituted C 4  alkylene or alkenylene, wherein one or more methylene units are optionally and independently replaced by —O—, —S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —S(O)—, —S(O) 2 —, or 
                         
and each of R′ and Cy′ is independently as defined above and described herein.
 
     In some embodiments, -L 3 -G- is 
     
       
         
         
             
             
         
       
     
     In some embodiments, L 3  is an optionally substituted C 3  alkylene or alkenylene, wherein one or more methylene units are optionally and independently replaced by —O—, —S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —S(O)—, —S(O) 2 —, or 
                         
and each of R′ and Cy′ is independently as defined above and described herein.
 
     In some embodiments, -L 3 -G- is 
     
       
         
         
             
             
         
       
     
     In some embodiments, L is 
                         
In some embodiments, L is
 
                         
In some embodiments, L is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, L 3  is an optionally substituted C 2  alkylene or alkenylene, wherein one or more methylene units are optionally and independently replaced by —O—, —S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —S(O)—, —S(O) 2 —, or 
                         
and each of R′ and Cy′ is independently as defined above and described herein.
 
     In some embodiments, -L 3 -G- is 
                         
wherein each of G and Cy′ is independently as defined above and described herein. In some embodiments, L is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, L is -L 4 -G-, wherein L 4  is an optionally substituted C 1 -C 2  alkylene; and G is as defined above and described herein. In some embodiments, L is -L 4 -G-, wherein L 4  is an optionally substituted C 1 -C 2  alkylene; G is as defined above and described herein; and G is connected to R 1 . In some embodiments, L is -L 4 -G-, wherein L 4  is an optionally substituted methylene; G is as defined above and described herein; and G is connected to R 1 . In some embodiments, L is -L 4 -G-, wherein L 4  is methylene; G is as defined above and described herein; and G is connected to R 1 . In some embodiments, L is -L 4 -G-, wherein L 4  is an optionally substituted —(CH 2 ) 2 —; G is as defined above and described herein; and G is connected to R 1 . In some embodiments, L is -L 4 -G-, wherein L 4  is —(CH 2 ) 2 —; G is as defined above and described herein; and G is connected to R 1 . 
     In some embodiments, L is 
                         
wherein G is as defined above and described herein, and G is connected to R 1 . In some embodiments, L is
 
                         
wherein G is as defined above and described herein, and G is connected to R 1 . In some embodiments, L is
 
                         
wherein G is as defined above and described herein, and G is connected to R 1 . In some embodiments, L is
 
                         
wherein the sulfur atom is connected to R 1 . In some embodiments, L is
 
                         
wherein the oxygen atom is connected to R 1 .
 
     In some embodiments, L is 
                         
wherein G is as defined above and described herein.
 
     In some embodiments, L is —S—R L3 — or —S—C(O)—R L3 —, wherein R L3  is an optionally substituted, linear or branched, C 1 -C 9  alkylene, wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—, wherein each of R′ and -Cy- is independently as defined above and described herein. In some embodiments, L is —S—R L3 — or —S—C(O)—R L3 —, wherein R L3  is an optionally substituted C 1 -C 6  alkylene. In some embodiments, L is —S—R L3 — or —S—C(O)—R L3 —, wherein R L3  is an optionally substituted C 1 -C 6  alkenylene. In some embodiments, L is —S—R L3 — or —S—C(O)—R L3 —, wherein R L3  is an optionally substituted C 1 -C 6  alkylene wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkenylene, arylene, or heteroarylene. In some embodiments, In some embodiments, R L3  is an optionally substituted —S—(C 1 -C 6  alkenylene)-, —S—(C 1 -C 6  alkylene)-, —S—(C 1 -C 6  alkylene)-arylene-(C 1 -C 6  alkylene)-, —S—CO-arylene-(C 1 -C 6  alkylene)-, or —S—CO—(C 1 -C 6  alkylene)-arylene-(C 1 -C 6  alkylene)-. 
     In some embodiments, L is 
     
       
         
         
             
             
         
       
     
     In some embodiments, L is 
                         
In some embodiments, L is
 
                         
In some embodiments,
 
     
       
         
         
             
             
         
       
     
     In some embodiments, the sulfur atom in the L embodiments described above and herein is connected to X. In some embodiments, the sulfur atom in the L embodiments described above and herein is connected to R 1 . 
     As defined generally above and herein, R 1  is halogen, R, or an optionally substituted C 1 -C 50  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—, wherein each variable is independently as defined above and described herein. In some embodiments, R 1  is halogen, R, or an optionally substituted C 1 -C 10  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—, wherein each variable is independently as defined above and described herein. 
     In some embodiments, R 1  is hydrogen. In some embodiments, R 1  is halogen. In some embodiments, R 1  is —F. In some embodiments, R′ is —Cl. In some embodiments, R 1  is —Br. In some embodiments, R 1  is —I. 
     In some embodiments, R 1  is R wherein R is as defined above and described herein. 
     In some embodiments, R 1  is hydrogen. In some embodiments, R 1  is an optionally substituted group selected from C 1 -C 50  aliphatic, phenyl, carbocyclyl, aryl, heteroaryl, or heterocyclyl. 
     In some embodiments, R 1  is an optionally substituted C 1 -C 50  aliphatic. In some embodiments, R 1  is an optionally substituted C 1 -C 10  aliphatic. In some embodiments, R 1  is an optionally substituted C 1 -C 6  aliphatic. In some embodiments, R 1  is an optionally substituted C 1 -C 6  alkyl. In some embodiments, R 1  is optionally substituted, linear or branched hexyl. In some embodiments, R 1  is optionally substituted, linear or branched pentyl. In some embodiments, R 1  is optionally substituted, linear or branched butyl. In some embodiments, R 1  is optionally substituted, linear or branched propyl. In some embodiments, R 1  is optionally substituted ethyl. In some embodiments, R 1  is optionally substituted methyl. 
     In some embodiments, R 1  is optionally substituted phenyl. In some embodiments, R 1  is substituted phenyl. In some embodiments, R 1  is phenyl. 
     In some embodiments, R 1  is optionally substituted carbocyclyl. In some embodiments, R 1  is optionally substituted C 3 -C 10  carbocyclyl. In some embodiments, R 1  is optionally substituted monocyclic carbocyclyl. In some embodiments, R 1  is optionally substituted cycloheptyl. In some embodiments, R 1  is optionally substituted cyclohexyl. In some embodiments, R 1  is optionally substituted cyclopentyl. In some embodiments, R 1  is optionally substituted cyclobutyl. In some embodiments, R 1  is an optionally substituted cyclopropyl. In some embodiments, R 1  is optionally substituted bicyclic carbocyclyl. 
     In some embodiments, R 1  is an optionally substituted C 1 -C 50  polycyclic hydrocarbon. In some embodiments, R 1  is an optionally substituted C 1 -C 50  polycyclic hydrocarbon wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—, wherein each variable is independently as defined above and described herein. In some embodiments, R 1  is optionally substituted 
                         
In some embodiments, R 1  is
 
                         
In some embodiments, R 1  is optionally substituted
 
     
       
         
         
             
             
         
       
     
     In some embodiments, R 1  is an optionally substituted C 1 -C 50  aliphatic comprising one or more optionally substituted polycyclic hydrocarbon moieties. In some embodiments, R 1  is an optionally substituted C 1 -C 50  aliphatic comprising one or more optionally substituted polycyclic hydrocarbon moieties, wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—, wherein each variable is independently as defined above and described herein. In some embodiments, R 1  is an optionally substituted C 1 -C 50  aliphatic comprising one or more optionally substituted 
                         
In some embodiments, R 1  is
 
                         
In some embodiments, R 1  is
 
                         
In some embodiments, R 1  is
 
                         
In some embodiments, R 1  is
 
                         
In some embodiments, R 1  is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, R 1  is an optionally substituted aryl. In some embodiments, R 1  is an optionally substituted bicyclic aryl ring. 
     In some embodiments, R 1  is an optionally substituted heteroaryl. In some embodiments, R 1  is an optionally substituted 5-6 membered monocyclic heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, sulfur, or oxygen. In some embodiments, R 1  is a substituted 5-6 membered monocyclic heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is an unsubstituted 5-6 membered monocyclic heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, sulfur, or oxygen. 
     In some embodiments, R 1  is an optionally substituted 5 membered monocyclic heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, oxygen or sulfur. In some embodiments, R 1  is an optionally substituted 6 membered monocyclic heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     In some embodiments, R 1  is an optionally substituted 5-membered monocyclic heteroaryl ring having 1 heteroatom selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is selected from pyrrolyl, furanyl, or thienyl. 
     In some embodiments, R 1  is an optionally substituted 5-membered heteroaryl ring having 2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In certain embodiments, R 1  is an optionally substituted 5-membered heteroaryl ring having 1 nitrogen atom, and an additional heteroatom selected from sulfur or oxygen. Exemplary R 1  groups include optionally substituted pyrazolyl, imidazolyl, thiazolyl, isothiazolyl, oxazolyl or isoxazolyl. 
     In some embodiments, R 1  is a 6-membered heteroaryl ring having 1-3 nitrogen atoms. In other embodiments, R 1  is an optionally substituted 6-membered heteroaryl ring having 1-2 nitrogen atoms. In some embodiments, R 1  is an optionally substituted 6-membered heteroaryl ring having 2 nitrogen atoms. In certain embodiments, R 1  is an optionally substituted 6-membered heteroaryl ring having 1 nitrogen. Exemplary R 1  groups include optionally substituted pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazinyl, or tetrazinyl. 
     In certain embodiments, R 1  is an optionally substituted 8-10 membered bicyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is an optionally substituted 5,6-fused heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In other embodiments, R 1  is an optionally substituted 5,6-fused heteroaryl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In certain embodiments, R 1  is an optionally substituted 5,6-fused heteroaryl ring having 1 heteroatom independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is an optionally substituted indolyl. In some embodiments, R 1  is an optionally substituted azabicyclo[3.2.1]octanyl. In certain embodiments, R 1  is an optionally substituted 5,6-fused heteroaryl ring having 2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is an optionally substituted azaindolyl. In some embodiments, R 1  is an optionally substituted benzimidazolyl. In some embodiments, R 1  is an optionally substituted benzothiazolyl. In some embodiments, R 1  is an optionally substituted benzoxazolyl. In some embodiments, R 1  is an optionally substituted indazolyl. In certain embodiments, R 1  is an optionally substituted 5,6-fused heteroaryl ring having 3 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     In certain embodiments, R 1  is an optionally substituted 6,6-fused heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is an optionally substituted 6,6-fused heteroaryl ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In other embodiments, R 1  is an optionally substituted 6,6-fused heteroaryl ring having 1 heteroatom independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is an optionally substituted quinolinyl. In some embodiments, R 1  is an optionally substituted isoquinolinyl. According to one aspect, R 1  is an optionally substituted 6,6-fused heteroaryl ring having 2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is a quinazoline or a quinoxaline. 
     In some embodiments, R 1  is an optionally substituted heterocyclyl. In some embodiments, R 1  is an optionally substituted 3-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is a substituted 3-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is an unsubstituted 3-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     In some embodiments, R 1  is an optionally substituted heterocyclyl. In some embodiments, R 1  is an optionally substituted 6 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is an optionally substituted 6 membered partially unsaturated heterocyclic ring having 2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is an optionally substituted 6 membered partially unsaturated heterocyclic ring having 2 oxygen atoms. 
     In certain embodiments, R 1  is a 3-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In certain embodiments, R 1  is oxiranyl, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, oxepaneyl, aziridineyl, azetidineyl, pyrrolidinyl, piperidinyl, azepanyl, thiiranyl, thietanyl, tetrahydrothiophenyl, tetrahydrothiopyranyl, thiepanyl, dioxolanyl, oxathiolanyl, oxazolidinyl, imidazolidinyl, thiazolidinyl, dithiolanyl, dioxanyl, morpholinyl, oxathianyl, piperazinyl, thiomorpholinyl, dithianyl, dioxepanyl, oxazepanyl, oxathiepanyl, dithiepanyl, diazepanyl, dihydrofuranonyl, tetrahydropyranonyl, oxepanonyl, pyrolidinonyl, piperidinonyl, azepanonyl, dihydrothiophenonyl, tetrahydrothiopyranonyl, thiepanonyl, oxazolidinonyl, oxazinanonyl, oxazepanonyl, dioxolanonyl, dioxanonyl, dioxepanonyl, oxathiolinonyl, oxathianonyl, oxathiepanonyl, thiazolidinonyl, thiazinanonyl, thiazepanonyl, imidazolidinonyl, tetrahydropyrimidinonyl, diazepanonyl, imidazolidinedionyl, oxazolidinedionyl, thiazolidinedionyl, dioxolanedionyl, oxathiolanedionyl, piperazinedionyl, morpholinedionyl, thiomorpholinedionyl, tetrahydropyranyl, tetrahydrofuranyl, morpholinyl, thiomorpholinyl, piperidinyl, piperazinyl, pyrrolidinyl, tetrahydrothiophenyl, or tetrahydrothiopyranyl. In some embodiments, R 1  is an optionally substituted 5 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. 
     In certain embodiments, R 1  is an optionally substituted 5-6 membered partially unsaturated monocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In certain embodiments, R 1  is an optionally substituted tetrahydropyridinyl, dihydrothiazolyl, dihydrooxazolyl, or oxazolinyl group. 
     In some embodiments, R 1  is an optionally substituted 8-10 membered bicyclic saturated or partially unsaturated heterocyclic ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R 1  is an optionally substituted indolinyl. In some embodiments, R 1  is an optionally substituted isoindolinyl. In some embodiments, R 1  is an optionally substituted 1, 2, 3, 4-tetrahydroquinoline. In some embodiments, R 1  is an optionally substituted 1, 2, 3, 4-tetrahydroisoquinoline. 
     In some embodiments, R 1  is an optionally substituted C 1 -C 10  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—, wherein each variable is independently as defined above and described herein. In some embodiments, R 1  is an optionally substituted C 1 -C 10  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —OC(O)—, or —C(O)O—, wherein each R′ is independently as defined above and described herein. In some embodiments, R′ is an optionally substituted C 1 -C 10  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —OC(O)—, or —C(O)O—, wherein each R′ is independently as defined above and described herein. 
     In some embodiments, R 1  is 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     In some embodiments, R 1  is CH 3 —, 
     
       
         
         
             
             
         
       
     
     In some embodiments, R 1  comprises a terminal optionally substituted —(CH 2 ) 2 — moiety which is connected to L. Exemplary such R 1  groups are depicted below: 
     
       
         
         
             
             
         
       
     
     In some embodiments, R 1  comprises a terminal optionally substituted —(CH 2 )— moiety which is connected to L. Exemplary such R 1  groups are depicted below: 
     
       
         
         
             
             
         
       
     
     In some embodiments, R 1  is —S—R L2 , wherein R L2  is an optionally substituted C 1 -C 9  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—, and each of R′ and -Cy- is independently as defined above and described herein. In some embodiments, R 1  is —S—R L2 , wherein the sulfur atom is connected with the sulfur atom in L group. 
     In some embodiments, R 1  is —C(O)—R L2 , wherein R L2  is an optionally substituted C 1 -C 9  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—, and each of R′ and -Cy- is independently as defined above and described herein. In some embodiments, R 1  is —C(O)—R L2 , wherein the carbonyl group is connected with G in L group. In some embodiments, R 1  is —C(O)—R L2 , wherein the carbonyl group is connected with the sulfur atom in L group. 
     In some embodiments, R L2  is optionally substituted C 1 -C 9  aliphatic. In some embodiments, R L2  is optionally substituted C 1 -C 9  alkyl. In some embodiments, R L2  is optionally substituted C 1 -C 9  alkenyl. In some embodiments, R L2  is optionally substituted C 1 -C 9  alkynyl. In some embodiments, R L2  is an optionally substituted C 1 -C 9  aliphatic wherein one or more methylene units are optionally and independently replaced by -Cy- or —C(O)—. In some embodiments, R L2  is an optionally substituted C 1 -C 9  aliphatic wherein one or more methylene units are optionally and independently replaced by -Cy-. In some embodiments, R L2  is an optionally substituted C 1 -C 9  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted heterocycylene. In some embodiments, R L2  is an optionally substituted C 1 -C 9  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted arylene. In some embodiments, R L2  is an optionally substituted C 1 -C 9  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted heteroarylene. In some embodiments, R L2  is an optionally substituted C 1 -C 9  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 3 -C 10  carbocyclylene. In some embodiments, R L2  is an optionally substituted C 1 -C 9  aliphatic wherein two methylene units are optionally and independently replaced by -Cy- or —C(O)—. In some embodiments, R L2  is an optionally substituted C 1 -C 9  aliphatic wherein two methylene units are optionally and independently replaced by -Cy- or —C(O)—. Exemplary R L2  groups are depicted below: 
     
       
         
         
             
             
         
       
     
     In some embodiments, R 1  is hydrogen, or an optionally substituted group selected from 
                         
—S—(C 1 -C 10  aliphatic), C 1 -C 10  aliphatic, aryl, C 1 -C 6  heteroalkyl, heteroaryl and heterocyclyl. In some embodiments, R 1  is
 
                         
—S—(C 1 -C 10  aliphatic). In some embodiments, R 1  is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, R 1  is an optionally substituted group selected from —S—(C 1 -C 6  aliphatic), C 1 -C 10  aliphatic, C 1 -C 6  heteroaliphatic, aryl, heterocyclyl and heteroaryl. 
     In some embodiments, R 1  is 
     
       
         
         
             
             
         
       
     
     In some embodiments, the sulfur atom in the R 1  embodiments described above and herein is connected with the sulfur atom, G, E, or —C(O)— moiety in the L embodiments described above and herein. In some embodiments, the —C(O)— moiety in the R 1  embodiments described above and herein is connected with the sulfur atom, G, E, or —C(O)— moiety in the L embodiments described above and herein. 
     In some embodiments, -L-R 1  is any combination of the L embodiments and R 1  embodiments described above and herein. 
     In some embodiments, -L-R 1  is -L 3 -G-R 1  wherein each variable is independently as defined above and described herein. 
     In some embodiments, -L-R 1  is -L 4 -G-R 1  wherein each variable is independently as defined above and described herein. 
     In some embodiments, -L-R 1  is -L 3 -G-S—R L2 , wherein each variable is independently as defined above and described herein. 
     In some embodiments, -L-R 1  is -L 3 -G-C(O)—R L2 , wherein each variable is independently as defined above and described herein. 
     In some embodiments, -L-R 1  is 
                         
wherein R L2  is an optionally substituted C 1 -C 9  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—, and each G is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  is —R L3 —S—S—R L2 , wherein each variable is independently as defined above and described herein. In some embodiments, -L-R 1  is —R L3 —C(O)—S—S—R L2 , wherein each variable is independently as defined above and described herein. 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, -L-R 1  has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, L has the structure of: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments —X-L-R 1  has the structure of: 
                         
wherein:
     the phenyl ring is optionally substituted, and   each of R 1  and X is independently as defined above and described herein.   

     In some embodiments, -L-R 1  is 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     In some embodiments, -L-R 1  is: 
     
       
         
         
             
             
         
       
     
     In some embodiments, -L-R 1  is CH 3 —, 
                         
In some embodiments, -L-R 1  is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, -L-R 1  comprises a terminal optionally substituted —(CH 2 ) 2 — moiety which is connected to X. In some embodiments, -L-R 1  comprises a terminal —(CH 2 ) 2 — moiety which is connected to X. Exemplary such -L-R 1  moieties are depicted below: 
     
       
         
         
             
             
         
       
     
     In some embodiments, -L-R 1  comprises a terminal optionally substituted —(CH 2 )— moiety which is connected to X. In some embodiments, -L-R 1  comprises a terminal —(CH 2 )— moiety which is connected to X. Exemplary such -L-R 1  moieties are depicted below: 
     
       
         
         
             
             
         
       
     
     In some embodiments, -L-R 1  is 
     
       
         
         
             
             
         
       
     
     In some embodiments, -L-R 1  is CH 3 —, 
                         
and X is —S—.
 
     In some embodiments, -L-R 1  is CH 3 —, 
                         
X is —S—, W is O, Y is —O—, and Z is —O—.
 
     In some embodiments, R 1  is 
                         
or —S—(C 1 -C 10  aliphatic).
 
     In some embodiments, R 1  is 
     
       
         
         
             
             
         
       
     
     In some embodiments, X is —O— or —S—, and R 1  is 
                         
or —S—(C 1 -C 10  aliphatic).
 
     In some embodiments, X is —O— or —S—, and R 1  is, 
                         
—S—(C 1 -C 10  aliphatic) or —S—(C 1 -C 50  aliphatic).
 
     In some embodiments, L is a covalent bond and -L-R 1  is R 1 . 
     In some embodiments, -L-R 1  is not hydrogen. 
     In some embodiments, —X-L-R 1  is R 1  is 
                         
—S—(C 1 -C 10  aliphatic) or —S—(C 1 -C 50  aliphatic).
 
     In some embodiments, —X-L-R 1  has the structure of 
                         
wherein the
 
                         
the moiety is optionally substituted. In some embodiments, —X-L-R 1  is
 
                         
In some embodiments, —X-L-R 1  is
 
                         
In some embodiments, —X-L-R 1  is
 
                         
In some embodiments, —X-L-R 1  has the structure of
 
                         
wherein X′ is O or S, Y′ is —O—, —S— or —NR′—, and the
 
                         
moiety is optionally substituted. In some embodiments, Y′ is —O—, —S— or —NH—. In some embodiments,
 
                         
In some embodiments,
 
                         
In some embodiments,
 
                         
In some embodiments, —X-L-R 1  has the structure of
 
                         
wherein X′ is O or S, and the
 
                         
moiety is optionally substituted. In some embodiments,
 
                         
In some embodiments, —X-L-R 1  is
 
                         
wherein the
 
                         
is optionally substituted. In some embodiments, —X-L-R 1  is
 
                         
wherein the
 
                         
is substituted. In some embodiments, —X-L-R 1  is
 
                         
wherein the
 
                         
is unsubstituted.
 
     In some embodiments, —X-L-R 1  is R 1 —C(O)—S-L x -S—, wherein L x  is an optionally substituted group selected from 
                         
In some embodiments, L x  is
 
                         
and
 
In some embodiments, —X-L-R 1  is (CH 3 ) 3 C—S—S-L x -S—. In some embodiments, —X-L-R 1  is R 1 —C(═X′)—Y′—C(R) 2 —S-L x -S—. In some embodiments, —X-L-R 1  is R—C(═X′)—Y′—CH 2 —S-L x -S—. In some embodiments, —X-L-R 1  is
 
     
       
         
         
             
             
         
       
     
     As will be appreciated by a person skilled in the art, many of the —X-L-R 1  groups described herein are cleavable and can be converted to —X −  after administration to a subject. In some embodiments, —X-L-R 1  is cleavable. In some embodiments, —X-L-R 1  is —S-L-R 1 , and is converted to —S −  after administration to a subject. In some embodiments, the conversion is promoted by an enzyme of a subject. As appreciated by a person skilled in the art, methods of determining whether the —S-L-R 1  group is converted to —S −  after administration is widely known and practiced in the art, including those used for studying drug metabolism and pharmacokinetics. 
     In some embodiments, the internucleotidic linkage having the structure of formula I is 
     
       
         
         
             
             
         
       
     
     In some embodiments, the internucleotidic linkage of formula I has the structure of formula I-a: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, the internucleotidic linkage of formula I has the structure of formula I-b: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, the internucleotidic linkage of formula I is an phosphorothioate triester linkage having the structure of formula I-c: 
                         
wherein:
     P* is an asymmetric phosphorus atom and is either Rp or Sp;   L is a covalent bond or an optionally substituted, linear or branched C 1 -C 10  alkylene, wherein one or more methylene units of L are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—;   R 1  is halogen, R, or an optionally substituted C 1 -C 50  aliphatic wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkylene, C 1 -C 6  alkenylene, —C≡C—, —C(R′) 2 —, -Cy-, —O—, —S—, —S—S—, —N(R′)—, —C(O)—, —C(S)—, —C(NR′)—, —C(O)N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)—, —N(R′)C(O)O—, —OC(O)N(R′)—, —S(O)—, —S(O) 2 —, —S(O) 2 N(R′)—, —N(R′)S(O) 2 —, —SC(O)—, —C(O)S—, —OC(O)—, or —C(O)O—;   each R′ is independently —R, —C(O)R, —CO 2 R, or —SO 2 R, or:
       two R′ on the same nitrogen are taken together with their intervening atoms to form an optionally substituted heterocyclic or heteroaryl ring, or   two R′ on the same carbon are taken together with their intervening atoms to form an optionally substituted aryl, carbocyclic, heterocyclic, or heteroaryl ring;   
       -Cy- is an optionally substituted bivalent ring selected from phenylene, carbocyclylene, arylene, heteroarylene, or heterocyclylene;   each R is independently hydrogen, or an optionally substituted group selected from C 1 -C 6  aliphatic phenyl carbocyclyl, aryl, heteroaryl, or heterocyclyl;   each   

                         
independently represents a connection to a nucleoside; and
     R 1  is not —H when L is a covalent bond.   

     In some embodiments, the internucleotidic linkage having the structure of formula I is 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     In some embodiments, the internucleotidic linkage having the structure of formula I-c is 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising one or more phosphate diester linkages, and one or more modified internucleotide linkages having the formula of I-a, I-b, or I-c. 
     In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising at least one phosphate diester internucleotidic linkage and at least one phosphorothioate triester linkage having the structure of formula I-c. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising at least one phosphate diester internucleotidic linkage and at least two phosphorothioate triester linkages having the structure of formula I-c. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising at least one phosphate diester internucleotidic linkage and at least three phosphorothioate triester linkages having the structure of formula I-c. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising at least one phosphate diester internucleotidic linkage and at least four phosphorothioate triester linkages having the structure of formula I-c. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising at least one phosphate diester internucleotidic linkage and at least five phosphorothioate triester linkages having the structure of formula I-c. 
     In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in any of the Appendixes of the application. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in Appendix A. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in Appendix B. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in Appendix C. In some embodiments, the present invention provides a chirally controlled oligonucleotide having a sequence found in any of the Appendixes of the application. In some embodiments, the present invention provides a chirally controlled oligonucleotide having a sequence found in Appendix A. In some embodiments, the present invention provides a chirally controlled oligonucleotide having a sequence found in Appendix B. In some embodiments, the present invention provides a chirally controlled oligonucleotide having a sequence found in Appendix C. 
     In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106). In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the said sequence has over 50% identity with GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106). In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the said sequence has over 60% identity with GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106). In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the said sequence has over 70% identity with GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106). In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the said sequence has over 80% identity with GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106). In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the said sequence has over 90% identity with GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106). In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the said sequence has over 95% identity with GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106). In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106). In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106). 
     In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage has a chiral linkage phosphorus. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage has the structure of formula I. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage has the structure of formula I. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage has the structure of formula I-c. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage has the structure of formula I-c. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage is 
                         
In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage is
 
                         
In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage is
 
                         
In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising a sequence found in GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage has a chiral linkage phosphorus. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage has the structure of formula I. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage has the structure of formula I. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage has the structure of formula I-c. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage has the structure of formula I-c. In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage is 
                         
In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage is
 
                         
In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage is
 
                         
In some embodiments, the present invention provides a chirally controlled oligonucleotide comprising the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage has a chiral linkage phosphorus. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage has the structure of formula I. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage has the structure of formula I. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage has the structure of formula I-c. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage has the structure of formula I-c. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage is 
                         
In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage is
 
                         
In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one internucleotidic linkage is
 
                         
In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each internucleotidic linkage is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one linkage phosphorus is Rp. It is understood by a person of ordinary skill in the art that in certain embodiments wherein the chirally controlled oligonucleotide comprises an RNA sequence, each T is independently and optionally replaced with U. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each linkage phosphorus is Rp. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one linkage phosphorus is Sp. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each linkage phosphorus is Sp. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a blockmer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a stereoblockmer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a P-modification blockmer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a linkage blockmer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is an altmer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a stereoaltmer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a P-modification altmer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a linkage altmer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a unimer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a stereounimer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a P-modification unimer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a linkage unimer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a gapmer. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein the oligonucleotide is a skipmer. 
     In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each cytosine is optionally and independently replaced by 5-methylcytosine. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein at least one cytosine is optionally and independently replaced by 5-methylcytosine. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106), wherein each cytosine is optionally and independently replaced by 5-methylcytosine. Exemplary chirally controlled oligonucleotides having the sequence of GCCTCAGTCTGCTTCGCACC (SEQ ID NO: 106) are depicted in Table 2, below: 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Exemplary chirally controlled oligonucleotides. 
               
            
           
           
               
               
               
               
            
               
                   
                 SEQ 
                   
                   
               
               
                 Oligo 
                 ID NO: 
                 Stereochemistry/Sequence 
                 Description 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 101 
                 106 
                 All-(Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                 All-R 
               
               
                   
               
               
                 102 
                 106 
                 All-(Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                 All-S 
               
               
                   
               
               
                 103 
                 106 
                 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, 
                 5R-9S-5R 
               
               
                   
                   
                 Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 104 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, 
                 5S-9R-5S 
               
               
                   
                   
                 Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 105 
                 106 
                 (Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Rp, Rp, Rp, 
                 1S-17R-1S 
               
               
                   
                   
                 Rp, Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 106 
                 106 
                 (Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, 
                 1R-17S-1R 
               
               
                   
                   
                 Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 107 
                 106 
                 (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, 
                 (R/S) 9 R 
               
               
                   
                   
                 Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 108 
                 106 
                 (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, 
                 (S/R) 9 S 
               
               
                   
                   
                 Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 109 
                 106 
                 (Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, 
                 3S-13R-3S 
               
               
                   
                   
                 Sp)d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 110 
                 106 
                 (Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, 
                 3R-13S-3R 
               
               
                   
                   
                 Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 111 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, 
                 18S/R 19   
               
               
                   
                   
                 Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 112 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, 
                 18S/R 9   
               
               
                   
                   
                 Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 113 
                 106 
                 (Sp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, 
                 18S/R 2   
               
               
                   
                   
                 Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 114 
                 106 
                 (Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, 
                 (RRS) 6 -R 
               
               
                   
                   
                 Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 115 
                 106 
                 (Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, 
                 S-(RRS) 6   
               
               
                   
                   
                 Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                   
               
               
                   
               
               
                 116 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] 
                 RR 
               
               
                   
               
               
                 122 
                 106 
                 All-(Rp)-d[Gs1Cs1Cs1Ts1Cs1As1Gs1Ts1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1Cs1As1Cs1C] 
                 All-R 
               
               
                   
               
               
                 123 
                 106 
                 (Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Rp, Rp, Rp, 
                 1S-17R-1S 
               
               
                   
                   
                 Rp, Sp)-d[Gs1Cs1Cs1Ts1Cs1As1Gs1Ts1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1Cs1As1Cs1C] 
                   
               
               
                   
               
               
                 124 
                 106 
                 All-(Sp)-d[Gs1Cs1Cs1Ts1Cs1As1Gs1Ts1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1Cs1As1Cs1C] 
                 All-S 
               
               
                   
               
               
                 125 
                   
                 All-(Rp)-d[5mCs1As1Ts1G] 
                 All-R 
               
               
                   
               
               
                 126 
                   
                 All-(Rp)-d[Cs2As2Gs2T] 
                 All-R 
               
               
                   
               
               
                 127 
                   
                 All-(Rp)-d[Cs3As3Gs3T] 
                 All-R 
               
               
                   
               
               
                 128 
                   
                 All-(Sp)-d[Cs4As4Gs4T] 
                 All-S 
               
               
                   
               
               
                 129 
                   
                 All-(Sp)-d[Cs5As5Gs5T] 
                 All-S 
               
               
                   
               
               
                 130 
                   
                 All-(Sp)-d[Cs6As6Gs6T] 
                 All-S 
               
               
                   
               
               
                 131 
                 106 
                 All-(Rp)-d[Gs7Cs7Cs7Ts7Cs7As7Gs7Ts7Cs7Ts7Gs7 
                 All-R 
               
               
                   
                   
                 Cs7Ts7Ts7Cs7Gs7Cs7As7Cs7C] 
                   
               
               
                   
               
               
                 132 
                 106 
                 All-(Sp)-d[Gs7Cs7Cs7Ts7Cs7As7Gs7Ts7Cs7Ts7Gs7 
                 All-S 
               
               
                   
                   
                 Cs7Ts7Ts7Cs7Gs7Cs7As7Cs7C] 
                   
               
               
                   
               
               
                 133 
                 106 
                 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, 
                 5R-9S-5R 
               
               
                   
                   
                 Rp)-d[Gs15mCs15mCs1Ts15mCs1As1Gs1Ts15mCs1Ts1 
                   
               
               
                   
                   
                 Gs15mCs1Ts1Ts15mCs1Gs15mCs1As15mCs15mC] 
                   
               
               
                   
               
               
                 134 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, 
                 5S-9R-5S 
               
               
                   
                   
                 Sp)-d[Gs15mCs15mCs1Ts15mCs1As1Gs1Ts15mCs1Ts1 
                   
               
               
                   
                   
                 Gs15mCs1Ts1Ts15mCs1Gs15mCs1As15mCs15mC] 
                   
               
               
                   
               
               
                 135 
                 108 
                 All-(Rp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] 
                 All-R 
               
               
                   
               
               
                 136 
                 108 
                 All-(Sp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] 
                 All-S 
               
               
                   
               
               
                 137 
                 108 
                 (Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp)- 
                 1S-9R-1S 
               
               
                   
                   
                 d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] 
                   
               
               
                   
               
               
                 138 
                 108 
                 (Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp)- 
                 2S-7R-2S 
               
               
                   
                   
                 d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] 
                   
               
               
                   
               
               
                 139 
                 108 
                 (Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp)- 
                 1R-9S-1R 
               
               
                   
                   
                 d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] 
                   
               
               
                   
               
               
                 140 
                 108 
                 (Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp)- 
                 2R-7S-2R 
               
               
                   
                   
                 d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] 
                   
               
               
                   
               
               
                 141 
                 108 
                 (Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp)- 
                 3S-5R-3S 
               
               
                   
                   
                 d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] 
                   
               
               
                   
               
               
                 142 
                 108 
                 (Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp)- 
                 3R-5S-3R 
               
               
                   
                   
                 d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] 
                   
               
               
                   
               
               
                 143 
                 108 
                 (Sp, Sp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Sp, Sp)- 
                 (SSR) 3 -SS 
               
               
                   
                   
                 d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] 
                   
               
               
                   
               
               
                 144 
                 108 
                 (Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp)- 
                 (RRS) 3 -RR 
               
               
                   
                   
                 d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] 
                   
               
               
                   
               
               
                 145 
                 109 
                 All-(Rp)-d[5mCs1Ts15mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1 
                 All-R 
               
               
                   
                   
                 Ts15mCs1Gs15mC] 
                   
               
               
                   
               
               
                 146 
                   
                 All-(Rp)-d[Gs15mCs1Ts1G] 
                 All-R 
               
               
                   
               
               
                 147 
                   
                 All-(Rp)-d[5mCs1As1Gs1T] 
                 All-R 
               
               
                   
               
               
                 148 
                 108 
                 All-(Rp)-d[5mCs2As2Gs2Ts25mCs2Ts2Gs25mCs2Ts2Ts25mCs2G] 
                 All-R 
               
               
                   
               
               
                 149 
                 108 
                 All-(Rp)-d[5mCs4As4Gs4Ts45mCs4Ts4Gs45mCs4Ts4Ts45mCs4G] 
                 All-R 
               
               
                   
               
               
                 150 
                   
                 All-(Rp)-d[TsCs1AsT] 
                 All-R 
               
               
                   
               
               
                 151 
                   
                 All-(Sp)-d[Cs1AsGs1T] 
                 All-S 
               
               
                   
               
               
                 152 
                   
                 All-(Sp)-d[Cs1AGs1T] 
                 All-S 
               
               
                   
               
               
                 153 
                   
                 All-(Sp)-d[CAs1GsT] 
                 All-S 
               
               
                   
               
               
                 154 
                   
                 All-(Rp)-d[Ts1Cs1As1T] 
                 All-R 
               
               
                   
               
               
                 155 
                   
                 All-(Rp)-d[Ts2Gs2As2C] 
                 All-R 
               
               
                   
               
               
                 156 
                   
                 All-(Sp)-d[Gs15mCs1Ts1G] 
                 All-S 
               
               
                   
               
               
                 157 
                   
                 All-(Sp)-d[5mCs1As1Gs1T] 
                 All-S 
               
               
                   
               
               
                 158 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCs1GsCsACsC] 
                   
               
               
                   
               
               
                 159 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, 
                 5S-9R-5S 
               
               
                   
                   
                 Sp)-d[Gs1Cs1Cs1Ts1CsAsGsTsCsTsGsCsTsTsCs1GsCs2As2Cs2C] 
                   
               
               
                   
               
               
                 160 
                 106 
                 All-(Rp)- 
                 All-R 
               
               
                   
                   
                 (Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs](Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 161 
                 106 
                 All-(Sp)- 
                 All-S 
               
               
                   
                   
                 (Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs](Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 162 
                 106 
                 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, 
                 5R-9S-5R 
               
               
                   
                   
                 Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs](Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 163 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, 
                 5S-9R-5S 
               
               
                   
                   
                 Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs](Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                 164 
                 106 
                 (Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Rp, Rp, Rp, 
                 1S-17R-1S 
               
               
                   
                   
                 Rp, Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 165 
                 106 
                 (Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, 
                 1R-17S-1R 
               
               
                   
                   
                 Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 166 
                 106 
                 (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, 
                 (R/S) 9 R 
               
               
                   
                   
                 Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 167 
                 106 
                 (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, 
                 (S/R) 9 S 
               
               
                   
                   
                 Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 168 
                 106 
                 (Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, 
                 3S-13R-3S 
               
               
                   
                   
                 Sp)(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 169 
                 106 
                 (Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, 
                 3R-13S-3R 
               
               
                   
                   
                 Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 170 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, 
                 18S/R 19   
               
               
                   
                   
                 Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 171 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, 
                 18S/R 9   
               
               
                   
                   
                 Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 172 
                 106 
                 (Sp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, 
                 18S/R 2   
               
               
                   
                   
                 Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 173 
                 106 
                 (Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, 
                 (RRS) 6 -R 
               
               
                   
                   
                 Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 174 
                 106 
                 (Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, 
                 S-(RRS) 6   
               
               
                   
                   
                 Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 175 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                 RR 
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) MOE   
                   
               
               
                   
               
               
                 176 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)(Gs15mCs15mCs1Ts15mCs1) MOE d[As1Gs1Ts15mCs1Ts1Gs15m 
                 RR 
               
               
                   
                   
                 Cs1Ts1Ts15mCs1] (Gs15mCs1As15mCs15mC) MOE   
                   
               
               
                   
               
               
                 177 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)(Gs15mCs15mCs1Ts15mCs1) MOE d[AGT5mCTG5mCTT5mC] 
                 RR 
               
               
                   
                   
                 (Gs25mCs2As25mCs25mC) MOE   
                   
               
               
                   
               
               
                 178 
                 106 
                 (Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, 
                 S-(RRS) 6   
               
               
                   
                   
                 Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs] 
                   
               
               
                   
                   
                 (Gs5mCsAs5mCs5mC) F  (F: 2-fluorodeoxyribose) 
                   
               
               
                   
               
               
                 179 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)d[Gs8Cs8Cs8Ts8Cs8As8Gs8Ts8Cs8Ts8Gs8Cs8Ts8Ts8Cs8Gs8Cs 
                 RR 
               
               
                   
                   
                 8As8Cs8C] 
                   
               
               
                   
               
               
                 180 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)d[Gs9Cs9Cs9Ts9Cs9As9Gs9Ts9Cs9Ts9Gs9Cs9Ts9Ts9Cs9Gs9Cs 
                 RR 
               
               
                   
                   
                 9As9Cs9C] 
                   
               
               
                   
               
               
                 181 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)d[Gs10Cs10Cs10Ts10Cs10As10Gs10Ts10Cs10Ts10Gs10Cs10Ts10 
                 RR 
               
               
                   
                   
                 Ts10Cs10Gs10Cs10As10Cs10C] 
                   
               
               
                   
               
               
                 182 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)d[Gs11Cs11Cs11Ts11Cs11As11Gs11Ts11Cs11Ts11Gs11Cs11Ts11 
                 RR 
               
               
                   
                   
                 Ts11Cs11Gs11Cs11As11Cs11C] 
                   
               
               
                   
               
               
                 183 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)d[Gs12Cs12Cs12Ts12Cs12As12Gs12Ts12Cs12Ts12Gs12Cs12Ts12 
                 RR 
               
               
                   
                   
                 Ts12Cs12Gs12Cs12As12Cs12C] 
                   
               
               
                   
               
               
                 184 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)d[Gs13Cs13Cs13Ts13Cs13As13Gs13Ts13Cs13Ts13Gs13Cs13Ts13 
                 RR 
               
               
                   
                   
                 Ts13Cs13Gs13Cs13As13Cs13C] 
                   
               
               
                   
               
               
                 185 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)d[Gs14Cs14Cs14Ts14Cs14As14Gs14Ts14Cs14Ts14Gs14Cs14Ts14 
                 RR 
               
               
                   
                   
                 Ts14Cs14Gs14Cs14As14Cs14C] 
                   
               
               
                   
               
               
                 186 
                 106 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)d[Gs15Cs15Cs15Ts15Cs15As15Gs15Ts15Cs15Ts15Gs15Cs15Ts15 
                 RR 
               
               
                   
                   
                 Ts15Cs15Gs15Cs15As15Cs15C] 
                   
               
               
                   
               
               
                 187 
                 110 
                 (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp 
                 RS-(RRS) 5 - 
               
               
                   
                   
                 Rp)d[GsCsCs1TsCsAs]GsUs2CsUsGsd[CsTs3TsCsGs]CsAs4CsC 
                 RR 
               
               
                   
               
               
                 188 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsACsC] 
                   
               
               
                   
               
               
                 189 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[Gs1Cs1Cs1Ts1Cs1As1Gs1Ts1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1CsACs 
                   
               
               
                   
                   
                 1C] 
                   
               
               
                   
               
               
                 190 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[Gs8Cs8Cs8Ts8Cs8As8Gs8Ts8Cs8Ts8Gs8Cs8Ts8Ts8Cs8Gs8Cs1A 
                   
               
               
                   
                   
                 Cs8C] 
                   
               
               
                   
               
               
                 191 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[Gs9Cs9Cs9Ts9Cs9As9Gs9Ts9Cs9Ts9Gs9Cs9Ts9Ts9Cs9Gs9Cs1A 
                   
               
               
                   
                   
                 Cs9C] 
                   
               
               
                   
               
               
                 192 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[Gs10Cs10Cs10Ts10Cs10As10Gs10Ts10Cs10Ts10Gs10Cs10Ts10Ts 
                   
               
               
                   
                   
                 10Cs10Gs10Cs1ACs10C] 
                   
               
               
                   
               
               
                 193 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[Gs11Cs11Cs11Ts11Cs11As11Gs11Ts11Cs11Ts11Gs11Cs11Ts11Ts 
                   
               
               
                   
                   
                 11Cs11Gs11Cs1ACs11C] 
                   
               
               
                   
               
               
                 194 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[Gs12Cs12Cs12Ts12Cs12As12Gs12Ts12Cs12Ts12Gs12Cs12Ts12Ts 
                   
               
               
                   
                   
                 12Cs12Gs12Cs1ACs12C] 
                   
               
               
                   
               
               
                 195 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[Gs13Cs13Cs13Ts13Cs13As13Gs13Ts13Cs13Ts13Gs13Cs13Ts13Ts 
                   
               
               
                   
                   
                 13Cs13Gs13Cs1ACs13C] 
                   
               
               
                   
               
               
                 196 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[Gs14Cs14Cs14Ts14Cs14As14Gs14Ts14Cs14Ts14Gs14Cs14Ts14Ts 
                   
               
               
                   
                   
                 14Cs14Gs14Cs1ACs14C] 
                   
               
               
                   
               
               
                 197 
                 106 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 d[Gs15Cs15Cs15Ts15Cs15As15Gs15Ts15Cs15Ts15Gs15Cs15Ts15Ts 
                   
               
               
                   
                   
                 15Cs15Gs15Cs1ACs15C] 
                   
               
               
                   
               
               
                 198 
                 111 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 GsCsCsUsCsAsGsUsCsUsGsCsUsUsCsGsCsACsC 
                   
               
               
                   
               
               
                 199 
                 111 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 Gs1Cs1Cs1Us1Cs1As1Gs1Us1Cs1Us1Gs1Cs1Us1Us1Cs1Gs1CsACs 
                   
               
               
                   
                   
                 1C 
                   
               
               
                   
               
               
                 200 
                 111 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 Gs8Cs8Cs8Us8Cs8As8Gs8Us8Cs8Us8Gs8Cs8Us8Us8Cs8Gs8Cs1AC 
                   
               
               
                   
                   
                 s8C 
                   
               
               
                   
               
               
                 201 
                 111 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 Gs9Cs9Cs9Us9Cs9As9Gs9Us9Cs9Us9Gs9Cs9Us9Us9Cs9Gs9Cs1AC 
                   
               
               
                   
                   
                 s9C 
                   
               
               
                   
               
               
                 202 
                 111 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 Gs10Cs10Cs10Us10Cs10As10Gs10Us10Cs10Us10Gs10Cs10Us10Us 
                   
               
               
                   
                   
                 10Cs10Gs10Cs1ACs10C 
                   
               
               
                   
               
               
                 203 
                 111 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 Gs11Cs11Cs11Us11Cs11As11Gs11Us11Cs11Us11Gs11Cs11Us11Us 
                   
               
               
                   
                   
                 11Cs11Gs11Cs1ACs11C 
                   
               
               
                   
               
               
                 204 
                 111 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 Gs12Cs12Cs12Us12Cs12As12Gs12Us12Cs12Us12Gs12Cs12Us12Us 
                   
               
               
                   
                   
                 12Cs12Gs12Cs1ACs12C 
                   
               
               
                   
               
               
                 205 
                 111 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 Gs13Cs13Cs13Us13Cs13As13Gs13Us13Cs13Us13Gs13Cs13Us13Us 
                   
               
               
                   
                   
                 13Cs13Gs13Cs1ACs13C 
                   
               
               
                   
               
               
                 206 
                 111 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 Gs14Cs14Cs14Us14Cs14As14Gs14Us14Cs14Us14Gs14Cs14Us14Us 
                   
               
               
                   
                   
                 14Cs14Gs14Cs1ACs14C 
                   
               
               
                   
               
               
                 207 
                 111 
                 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- 
                 5S-9R-4S 
               
               
                   
                   
                 Gs15Cs15Cs15Us15Cs15As15Gs15Us15Cs15Us15Gs15Cs15Us15Us 
                   
               
               
                   
                   
                 15Cs15Gs15Cs1ACs15C 
               
               
                   
               
            
           
         
       
     
     In some embodiments, a chirally controlled oligonucleotide is designed such that one or more nucleotides comprise a phosphorus modification prone to “autorelease” under certain conditions. That is, under certain conditions, a particular phosphorus modification is designed such that it self-cleaves from the oligonucleotide to provide, e.g., a phosphate diester such as those found in naturally occurring DNA and RNA. In some embodiments, such a phosphorus modification has a structure of —O-L-R 1 , wherein each of L and R 1  is independently as defined above and described herein. In some embodiments, an autorelease group comprises a morpholino group. In some embodiments, an autorelease group is characterized by the ability to deliver an agent to the internucleotidic phosphorus linker, which agent facilitates further modification of the phosphorus atom such as, e.g., desulfurization. In some embodiments, the agent is water and the further modification is hydrolysis to form a phosphate diester as is found in naturally occurring DNA and RNA. 
     In some embodiments, a chirally controlled oligonucleotide is designed such that the resulting pharmaceutical properties are improved through one or more particular modifications at phosphorus. It is well documented in the art that certain oligonucleotides are rapidly degraded by nucleases and exhibit poor cellular uptake through the cytoplasmic cell membrane (Poijarvi-Virta et al., Curr. Med. Chem. (2006), 13(28); 3441-65; Wagner et al., Med. Res. Rev. (2000), 20(6):417-51; Peyrottes et al., Mini Rev. Med. Chem. (2004), 4(4):395-408; Gosselin et al., (1996), 43(1):196-208; Bologna et al., (2002), Antisense &amp; Nucleic Acid Drug Development 12:33-41). For instance, Vives et al., (Nucleic Acids Research (1999), 27(20):4071-76) found that tert-butyl SATE pro-oligonucleotides displayed markedly increased cellular penetration compared to the parent oligonucleotide. 
     In some embodiments, a modification at a linkage phosphorus is characterized by its ability to be transformed to a phosphate diester, such as those present in naturally occurring DNA and RNA, by one or more esterases, nucleases, and/or cytochrome P450 enzymes, including but not limited to, those listed in Table 3, below. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Exemplary enzymes. 
               
            
           
           
               
               
               
            
               
                   
                 Family 
                 Gene 
               
               
                   
                   
               
               
                   
                 CYP1 
                 CYP1A1, CYP1A2, CYP1B1 
               
               
                   
                 CYP2 
                 CYP2A6, CYP2A7, CYP2A13, CYP2B6, 
               
               
                   
                   
                 CYP2C8, CYP2C9, CYP2C18, CYP2C19, 
               
               
                   
                   
                 CYP2D6, CYP2E1, CYP2F1, CYP2J2, 
               
               
                   
                   
                 CYP2R1, CYP2S1, CYP2U1, CYP2W1 
               
               
                   
                 CYP3 
                 CYP3A4, CYP3A5, CYP3A7, CYP3A43 
               
               
                   
                 CYP4 
                 CYP4A11, CYP4A22, CYP4B1, CYP4F2, 
               
               
                   
                   
                 CYP4F3, CYP4F8, CYP4F11, CYP4F12, 
               
               
                   
                   
                 CYP4F22, CYP4V2, CYP4X1, CYP4Z1 
               
               
                   
                 CYP5 
                 CYP5A1 
               
               
                   
                 CYP7 
                 CYP7A1, CYP7B1 
               
               
                   
                 CYP8 
                 CYP8A1 (prostacyclin synthase), CYP8B1 
               
               
                   
                   
                 (bile acid biosynthesis) 
               
               
                   
                 CYP11 
                 CYP11A1, CYP11B1, CYP11B2 
               
               
                   
                 CYP17 
                 CYP17A1 
               
               
                   
                 CYP19 
                 CYP19A1 
               
               
                   
                 CYP20 
                 CYP20A1 
               
               
                   
                 CYP21 
                 CYP21A2 
               
               
                   
                 CYP24 
                 CYP24A1 
               
               
                   
                 CYP26 
                 CYP26A1, CYP26B1, CYP26C1 
               
               
                   
                 CYP27 
                 CYP27A1 (bile acid biosynthesis), CYP27B1 
               
               
                   
                   
                 (vitamin D3 1-alpha hydroxylase, activates 
               
               
                   
                   
                 vitamin D3), CYP27C1 (unknown function) 
               
               
                   
                 CYP39 
                 CYP39A1 
               
               
                   
                 CYP46 
                 CYP46A1 
               
               
                   
                 CYP51 
                 CYP51A1 (lanosterol 14-alpha demethylase) 
               
               
                   
                   
               
            
           
         
       
     
     In some embodiments, a modification at phosphorus results in a P-modification moiety characterized in that it acts as a pro-drug, e.g., the P-modification moiety facilitates delivery of an oligonucleotide to a desired location prior to removal. For instance, in some embodiments, a P-modification moiety results from PEGylation at the linkage phosphorus. One of skill in the relevant arts will appreciate that various PEG chain lengths are useful and that the selection of chain length will be determined in part by the result that is sought to be achieved by PEGylation. For instance, in some embodiments, PEGylation is effected in order to reduce RES uptake and extend in vivo circulation lifetime of an oligonucleotide. 
     In some embodiments, a PEGylation reagent for use in accordance with the present invention is of a molecular weight of about 300 g/mol to about 100,000 g/mol. In some embodiments, a PEGylation reagent is of a molecular weight of about 300 g/mol to about 10,000 g/mol. In some embodiments, a PEGylation reagent is of a molecular weight of about 300 g/mol to about 5,000 g/mol. In some embodiments, a PEGylation reagent is of a molecular weight of about 500 g/mol. In some embodiments, a PEGylation reagent of a molecular weight of about 1000 g/mol. In some embodiments, a PEGylation reagent is of a molecular weight of about 3000 g/mol. In some embodiments, a PEGylation reagent is of a molecular weight of about 5000 g/mol. 
     In certain embodiments, a PEGylation reagent is PEG500. In certain embodiments, a PEGylation reagent is PEG1000. In certain embodiments, a PEGylation reagent is PEG3000. In certain embodiments, a PEGylation reagent is PEG5000. 
     In some embodiments, a P-modification moiety is characterized in that it acts as a PK enhancer, e.g., lipids, PEGylated lipids, etc. 
     In some embodiments, a P-modification moiety is characterized in that it acts as an agent which promotes cell entry and/or endosomal escape, such as a membrane-disruptive lipid or peptide. 
     In some embodiments, a P-modification moiety is characterized in that it acts as a targeting agent. In some embodiments, a P-modification moiety is or comprises a targeting agent. The phrase “targeting agent,” as used herein, is an entity that is associates with a payload of interest (e.g., with an oligonucleotide or oligonucleotide composition) and also interacts with a target site of interest so that the payload of interest is targeted to the target site of interest when associated with the targeting agent to a materially greater extent than is observed under otherwise comparable conditions when the payload of interest is not associated with the targeting agent. A targeting agent may be, or comprise, any of a variety of chemical moieties, including, for example, small molecule moieties, nucleic acids, polypeptides, carbohydrates, etc. Targeting agents are described further by Adarsh et al., “Organelle Specific Targeted Drug Delivery—A Review,” International Journal of Research in Pharmaceutical and Biomedical Sciences, 2011, p. 895. 
     Exemplary such targeting agents include, but are not limited to, proteins (e.g. Transferrin), oligopeptides (e.g., cyclic and acylic RGD-containing oligopeptides), antibodies (monoclonal and polyclonal antibodies, e.g. IgG, IgA, IgM, IgD, IgE antibodies), sugars/carbohydrates (e.g., monosaccharides and/or oligosaccharides (mannose, mannose-6-phosphate, galactose, and the like)), vitamins (e.g., folate), or other small biomolecules. In some embodiments, a targeting moiety is a steroid molecule (e.g., bile acids including cholic acid, deoxycholic acid, dehydrocholic acid; cortisone; digoxigenin; testosterone; cholesterol; cationic steroids such as cortisone having a trimethylaminomethyl hydrazide group attached via a double bond at the 3-position of the cortisone ring, etc.). In some embodiments, a targeting moiety is a lipophilic molecule (e.g., alicyclic hydrocarbons, saturated and unsaturated fatty acids, waxes, terpenes, and polyalicyclic hydrocarbons such as adamantine and buckminsterfullerenes). In some embodiments, a lipophilic molecule is a terpenoid such as vitamin A, retinoic acid, retinal, or dehydroretinal. In some embodiments, a targeting moiety is a peptide. 
     In some embodiments, a P-modification moiety is a targeting agent of formula —X-L-R 1  wherein each of X, L, and R 1  are as defined in Formula I above. 
     In some embodiments, a P-modification moiety is characterized in that it facilitates cell specific delivery. 
     In some embodiments, a P-modification moiety is characterized in that it falls into one or more of the above-described categories. For instance, in some embodiments, a P-modification moiety acts as a PK enhancer and a targeting ligand. In some embodiments, a P-modification moiety acts as a pro-drug and an endosomal escape agent. One of skill in the relevant arts would recognize that numerous other such combinations are possible and are contemplated by the present invention. 
     Nucleobases 
     In some embodiments, a nucleobase present in a provided oligonucleotide is a natural nucleobase or a modified nucleobase derived from a natural nucleobase. Examples include, but are not limited to, uracil, thymine, adenine, cytosine, and guanine having their respective amino groups protected by acyl protecting groups, 2-fluorouracil, 2-fluorocytosine, 5-bromouracil, 5-iodouracil, 2,6-diaminopurine, azacytosine, pyrimidine analogs such as pseudoisocytosine and pseudouracil and other modified nucleobases such as 8-substituted purines, xanthine, or hypoxanthine (the latter two being the natural degradation products). Exemplary modified nucleobases are disclosed in Chiu and Rana,  RNA,  2003, 9, 1034-1048, Limbach et al.  Nucleic Acids Research,  1994, 22, 2183-2196 and Revankar and Rao,  Comprehensive Natural Products Chemistry , vol. 7, 313. 
     Compounds represented by the following general formulae are also contemplated as modified nucleobases: 
                         
wherein R 8  is an optionally substituted, linear or branched group selected from aliphatic, aryl, aralkyl, aryloxylalkyl, carbocyclyl, heterocyclyl or heteroaryl group having 1 to 15 carbon atoms, including, by way of example only, a methyl, isopropyl, phenyl, benzyl, or phenoxymethyl group; and each of R 9  and R 10  is independently an optionally substituted group selected from linear or branched aliphatic, carbocyclyl, aryl, heterocyclyl and heteroaryl.
 
     Modified nucleobases also include expanded-size nucleobases in which one or more aryl rings, such as phenyl rings, have been added. Nucleic base replacements described in the Glen Research catalog (www.glenresearch.com); Krueger A T et al,  Acc. Chem. Res.,  2007, 40, 141-150; Kool, E T,  Acc. Chem. Res.,  2002, 35, 936-943; Benner S. A., et al.,  Nat. Rev. Genet.,  2005, 6, 553-543; Romesberg, F. E., et al.,  Curr. Opin. Chem. Biol.,  2003, 7, 723-733; Hirao, I.,  Curr. Opin. Chem. Biol.,  2006, 10, 622-627, are contemplated as useful for the synthesis of the nucleic acids described herein. Some examples of these expanded-size nucleobases are shown below: 
     
       
         
         
             
             
         
       
     
     Herein, modified nucleobases also encompass structures that are not considered nucleobases but are other moieties such as, but not limited to, corrin- or porphyrin-derived rings. Porphyrin-derived base replacements have been described in Morales-Rojas, H and Kool, E T,  Org. Lett.,  2002, 4, 4377-4380. Shown below is an example of a porphyrin-derived ring which can be used as a base replacement: 
     
       
         
         
             
             
         
       
     
     In some embodiments, modified nucleobases are of any one of the following structures, optionally substituted: 
     
       
         
         
             
             
         
       
     
     In some embodiments, a modified nucleobase is fluorescent. Exemplary such fluorescent modified nucleobases include phenanthrene, pyrene, stillbene, isoxanthine, isozanthopterin, terphenyl, terthiophene, benzoterthiophene, coumarin, lumazine, tethered stillbene, benzo-uracil, and naphtho-uracil, as shown below: 
     
       
         
         
             
             
         
       
     
     In some embodiments, a modified nucleobase is unsubstituted. In some embodiments, a modified nucleobase is substituted. In some embodiments, a modified nucleobase is substituted such that it contains, e.g., heteroatoms, alkyl groups, or linking moieties connected to fluorescent moieties, biotin or avidin moieties, or other protein or peptides. In some embodiments, a modified nucleobase is a “universal base” that is not a nucleobase in the most classical sense, but that functions similarly to a nucleobase. One representative example of such a universal base is 3-nitropyrrole. 
     In some embodiments, other nucleosides can also be used in the process disclosed herein and include nucleosides that incorporate modified nucleobases, or nucleobases covalently bound to modified sugars. Some examples of nucleosides that incorporate modified nucleobases include 4-acetylcytidine; 5-(carboxyhydroxylmethyl)uridine; 2′-O-methylcytidine; 5-carboxymethylaminomethyl-2-thiouridine; 5-carboxymethylaminomethyluridine; dihydrouridine; 2′-O-methylpseudouridine; beta,D-galactosylqueosine; 2′-O-methylguanosine; N 6 -isopentenyladenosine; 1-methyladenosine; 1-methylpseudouridine; 1-methylguanosine; 1-methylinosine; 2,2-dimethylguanosine; 2-methyladenosine; 2-methylguanosine; N 7 -methylguanosine; 3-methyl-cytidine; 5-methylcytidine; 5-hydroxymethylcytidine; 5-formylcytosine; 5-carboxylcytosine; N 6 -methyladenosine; 7-methylguanosine; 5-methylaminoethyluridine; 5-methoxyaminomethyl-2-thiouridine; beta,D-mannosylqueosine; 5-methoxycarbonylmethyluridine; 5-methoxyuridine; 2-methylthio-N 6 -isopentenyladenosine; N-((9-beta,D-ribofuranosyl-2-methylthiopurine-6-yl)carbamoyl)threonine; N-((9-beta,D-ribofuranosylpurine-6-yl)-N-methylcarbamoyl)threonine; uridine-5-oxyacetic acid methylester; uridine-5-oxyacetic acid (v); pseudouridine; queosine; 2-thiocytidine; 5-methyl-2-thiouridine; 2-thiouridine; 4-thiouridine; 5-methyluridine; 2′-O-methyl-5-methyluridine; and 2′-O-methyluridine. 
     In some embodiments, nucleosides include 6′-modified bicyclic nucleoside analogs that have either (R) or (S)-chirality at the 6′-position and include the analogs described in U.S. Pat. No. 7,399,845. In other embodiments, nucleosides include 5′-modified bicyclic nucleoside analogs that have either (R) or (S)-chirality at the 5′-position and include the analogs described in US Patent Application Publication No. 20070287831. 
     In some embodiments, a nucleobase or modified nucleobase comprises one or more biomolecule binding moieties such as e.g., antibodies, antibody fragments, biotin, avidin, streptavidin, receptor ligands, or chelating moieties. In other embodiments, a nucleobase or modified nucleobase is 5-bromouracil, 5-iodouracil, or 2,6-diaminopurine. In some embodiments, a nucleobase or modified nucleobase is modified by substitution with a fluorescent or biomolecule binding moiety. In some embodiments, the substituent on a nucleobase or modified nucleobase is a fluorescent moiety. In some embodiments, the substituent on a nucleobase or modified nucleobase is biotin or avidin. 
     Representative U.S. patents that teach the preparation of certain of the above noted modified nucleobases as well as other modified nucleobases include, but are not limited to, the above noted U.S. Pat. No. 3,687,808, as well as U.S. Pat. Nos. 4,845,205; 5,130,30; 5,134,066; 5,175,273; 5,367,066; 5,432,272; 5,457,187; 5,457,191; 5,459,255; 5,484,908; 5,502,177; 5,525,711; 5,552,540; 5,587,469; 5,594,121, 5,596,091; 5,614,617; 5,681,941; 5,750,692; 6,015,886; 6,147,200; 6,166,197; 6,222,025; 6,235,887; 6,380,368; 6,528,640; 6,639,062; 6,617,438; 7,045,610; 7,427,672; and 7,495,088, each of which is herein incorporated by reference in its entirety. 
     Sugars 
     The most common naturally occurring nucleotides are comprised of ribose sugars linked to the nucleobases adenosine (A), cytosine (C), guanine (G), and thymine (T) or uracil (U). Also contemplated are modified nucleotides wherein a phosphate group or linkage phosphorus in the nucleotides can be linked to various positions of a sugar or modified sugar. As non-limiting examples, the phosphate group or linkage phosphorus can be linked to the 2′, 3′, 4′ or 5′ hydroxyl moiety of a sugar or modified sugar. Nucleotides that incorporate modified nucleobases as described herein are also contemplated in this context. In some embodiments, nucleotides or modified nucleotides comprising an unprotected —OH moiety are used in accordance with methods of the present invention. 
     Other modified sugars can also be incorporated within a provided oligonucleotide. In some embodiments, a modified sugar contains one or more substituents at the 2′ position including one of the following: —F; —CF 3 , —CN, —N 3 , —NO, —NO 2 , —OR′, —SR′, or —N(R′) 2 , wherein each R′ is independently as defined above and described herein; —O—(C 1 -C 10  alkyl), —S—(C 1 -C 10  alkyl), —NH—(C 1 -C 10  alkyl), or —N(C 1 -C 10  alkyl) 2 ; —O—(C 2 -C 10  alkenyl), —S—(C 2 -C 10  alkenyl), —NH—(C 2 -C 10  alkenyl), or —N(C 2 -C 10  alkenyl) 2 ; —O—(C 2 -C 10  alkynyl), —S—(C 2 -C 10  alkynyl), —NH—(C 2 -C 10  alkynyl), or —N(C 2 -C 10  alkynyl) 2 ; or —O—(C 1 -C 10  alkylene)-O—(C 1 -C 10  alkyl), —O—(C 1 -C 10  alkylene)-NH—(C 1 -C 10  alkyl) or —O—(C 1 -C 10  alkylene)-NH(C 1 -C 10  alkyl) 2 , —NH—(C 1 -C 10  alkylene)-O—(C 1 -C 10  alkyl), or —N(C 1 -C 10  alkyl)C 1 -C 10  alkylene)-O—(C 1 -C 10  alkyl), wherein the alkyl, alkylene, alkenyl and alkynyl may be substituted or unsubstituted. Examples of substituents include, and are not limited to, —O(CH 2 ) n OCH 3 , and —O(CH 2 ) n NH 2 , wherein n is from 1 to about 10, MOE, DMAOE, DMAEOE. Also contemplated herein are modified sugars described in WO 2001/088198; and Martin et al.,  Helv. Chim. Acta,  1995, 78, 486-504. In some embodiments, a modified sugar comprises one or more groups selected from a substituted silyl group, an RNA cleaving group, a reporter group, a fluorescent label, an intercalator, a group for improving the pharmacokinetic properties of a nucleic acid, a group for improving the pharmacodynamic properties of a nucleic acid, or other substituents having similar properties. In some embodiments, modifications are made at one or more of the the 2′, 3′, 4′, 5′, or 6′ positions of the sugar or modified sugar, including the 3′ position of the sugar on the 3′-terminal nucleotide or in the 5′ position of the 5′-terminal nucleotide. 
     In some embodiments, the 2′-OH of a ribose is replaced with a substituent including one of the following: —H, —F; —CF 3 , —CN, —N 3 , —NO, —NO 2 , —OR′, —SR′, or —N(R′) 2 , wherein each R′ is independently as defined above and described herein; —O—(C 1 -C 10  alkyl), —S—(C 1 -C 10  alkyl), —NH—(C 1 -C 10  alkyl), or —N(C 1 -C 10  alkyl) 2 ; —O—(C 2 -C 10  alkenyl), —S—(C 2 -C 10  alkenyl), —NH—(C 2 -C 10  alkenyl), or —N(C 2 -C 10  alkenyl) 2 ; —O—(C 2 -C 10  alkynyl), —S—(C 2 -C 10  alkynyl), —NH—(C 2 -C 10  alkynyl), or —N(C 2 -C 10  alkynyl) 2 ; or —O—(C 1 -C 10  alkylene)-O—(C 1 -C 10  alkyl), —O—(C 1 -C 10  alkylene)-NH—(C 1 -C 10  alkyl) or —O—(C 1 -C 10  alkylene)-NH(C 1 -C 10  alkyl) 2 , —NH—(C 1 -C 10  alkylene)-O—(C 1 -C 10  alkyl), or —N(C 1 -C 10  alkyl)C 1 -C 10  alkylene)-O—(C 1 -C 10  alkyl), wherein the alkyl, alkylene, alkenyl and alkynyl may be substituted or unsubstituted. In some embodiments, the 2′-OH is replaced with —H (deoxyribose). In some embodiments, the 2′-OH is replaced with —F. In some embodiments, the 2′-OH is replaced with —OR′. In some embodiments, the 2′-OH is replaced with —OMe. In some embodiments, the 2′-OH is replaced with —OCH 2 CH 2 OMe. 
     Modified sugars also include locked nucleic acids (LNAs). In some embodiments, the locked nucleic acid has the structure indicated below. A locked nucleic acid of the structure below is indicated, wherein Ba represents a nucleobase or modified nucleobase as described herein, and wherein R 2s  is —OCH 2 C4′-. 
     
       
         
         
             
             
         
       
     
     In some embodiments, a modified sugar is an ENA such as those described in, e.g., Seth et al., J Am Chem Soc. 2010 Oct. 27; 132(42): 14942-14950. In some embodiments, a modified sugar is any of those found in an XNA (xenonucleic acid), for instance, arabinose, anhydrohexitol, threose, 2′fluoroarabinose, or cyclohexene. 
     Modified sugars include sugar mimetics such as cyclobutyl or cyclopentyl moieties in place of the pentofuranosyl sugar. Representative United States patents that teach the preparation of such modified sugar structures include, but are not limited to, U.S. Pat. Nos. 4,981,957; 5,118,800; 5,319,080; and 5,359,044. Some modified sugars that are contemplated include sugars in which the oxygen atom within the ribose ring is replaced by nitrogen, sulfur, selenium, or carbon. In some embodiments, a modified sugar is a modified ribose wherein the oxygen atom within the ribose ring is replaced with nitrogen, and wherein the nitrogen is optionally substituted with an alkyl group (e.g., methyl, ethyl, isopropyl, etc). 
     Non-limiting examples of modified sugars include glycerol, which form glycerol nucleic acid (GNA) analogues. One example of a GNA analogue is shown below and is described in Zhang, R et al.,  J. Am. Chem. Soc.,  2008, 130, 5846-5847; Zhang L, et al.,  J. Am. Chem. Soc.,  2005, 127, 4174-4175 and Tsai C H et al.,  PNAS,  2007, 14598-14603 (X=O − ): 
     
       
         
         
             
             
         
       
     
     Another example of a GNA derived analogue, flexible nucleic acid (FNA) based on the mixed acetal aminal of formyl glycerol, is described in Joyce G F et al.,  PNAS,  1987, 84, 4398-4402 and Heuberger B D and Switzer C,  J. Am. Chem. Soc.,  2008, 130, 412-413, and is shown below: 
     
       
         
         
             
             
         
       
     
     Additional non-limiting examples of modified sugars include hexopyranosyl (6′ to 4′), pentopyranosyl (4′ to 2′), pentopyranosyl (4′ to 3′), or tetrofuranosyl (3′ to 2′) sugars. In some embodiments, a hexopyranosyl (6′ to 4′) sugar is of any one of the following formulae: 
                         
wherein X s  corresponds to the P-modification group “—XLR 1 ” described herein and Ba is as defined herein.
 
     In some embodiments, a pentopyranosyl (4′ to 2′) sugar is of any one of the following formulae: 
                         
wherein X s  corresponds to the P-modification group “—XLR 1 ” described herein and Ba is as defined herein.
 
     In some embodiments, a pentopyranosyl (4′ to 3′) sugar is of any one of the following formulae: 
                         
wherein X s  corresponds to the P-modification group “—XLR 1 ” described herein and Ba is as defined herein.
 
     In some embodiments, a tetrofuranosyl (3′ to 2′) sugar is of either of the following formulae: 
                         
wherein X s  corresponds to the P-modification group “—XLR 1 ” described herein and Ba is as defined herein.
 
     In some embodiments, a modified sugar is of any one of the following formulae: 
                                           
wherein X s  corresponds to the P-modification group “—XLR 1 ” described herein and Ba is as defined herein.
 
     In some embodiments, one or more hydroxyl group in a sugar moiety is optionally and independently replaced with halogen, R′—N(R′) 2 , —OR′, or —SR′, wherein each R′ is independently as defined above and described herein. 
     In some embodiments, a sugar mimetic is as illustrated below, wherein X s  corresponds to the P-modification group “—XLR 1 ” described herein, Ba is as defined herein, and X 1  is selected from —S—, —Se—, —CH 2 —, —NMe-, —NEt- or —NiPr—. 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     In some embodiments, at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50% or more (e.g., 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or more), inclusive, of the sugars in a chirally controlled oligonucleotide composition are modified. In some embodiments, only purine residues are modified (e.g., about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50% or more [e.g., 55%, 60%/a, 65%, 70%, 75%, 80%, 85%, 90%, 95% or more] of the purine residues are modified). In some embodiments, only pyrimidine residues are modified (e.g., about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50% or more [e.g., 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or more] of the pyridimine residues are modified). In some embodiments, both purine and pyrimidine residues are modified. 
     Modified sugars and sugar mimetics can be prepared by methods known in the art, including, but not limited to: A. Eschenmoser, Science (1999), 284:2118; M. Bohringer et al, Helv. Chim. Acta (1992), 75:1416-1477; M. Egli et al, J. Am. Chem. Soc. (2006), 128(33):10847-56; A. Eschenmoser in  Chemical Synthesis: Gnosis to Prognosis , C. Chatgilialoglu and V. Sniekus, Ed., (Kluwer Academic, Netherlands, 1996), p. 293; K.-U. Schoning et al, Science (2000), 290:1347-1351; A. Eschenmoser et al, Helv. Chim. Acta (1992), 75:218; J. Hunziker et al, Helv. Chim. Acta (1993), 76:259; G. Otting et al, Helv. Chim. Acta (1993), 76:2701; K. Groebke et al, Helv. Chim. Acta (1998), 81:375; and A. Eschenmoser, Science (1999), 284:2118. Modifications to the 2′ modifications can be found in Verma, S. et al.  Annu. Rev. Biochem.  1998, 67, 99-134 and all references therein. Specific modifications to the ribose can be found in the following references: 2′-fluoro (Kawasaki et. al.,  J. Med. Chem.,  1993, 36, 831-841), 2′-MOE (Martin, P.  Helv. Chim. Acta  1996, 79, 1930-1938), “LNA” (Wengel,  J. Acc. Chem. Res.  1999, 32, 301-310). In some embodiments, a modified sugar is any of those described in PCT Publication No. WO2012/030683, incorporated herein by reference, and depicted in the  FIGS.  26 - 30    of the present application. 
     Oligonucleotides 
     In some embodiments, the present invention provides oligonucleotides and oligonucleotide compositions that are chirally controlled. For instance, in some embodiments, a provided composition contains predetermined levels of one or more individual oligonucleotide types, wherein an oligonucleotide type is defined by: 1) base sequence; 2) pattern of backbone linkages; 3) pattern of backbone chiral centers; and 4) pattern of backbone P-modifications. 
     In some embodiments, a provided oligonucleotide is a unimer. In some embodiments, a provided oligonucleotide is a P-modification unimer. In some embodiments, a provided oligonucleotide is a stereounimer. In some embodiments, a provided oligonucleotide is a stereounimer of configuration Rp. In some embodiments, a provided oligonucleotide is a stereounimer of configuration Sp. 
     In some embodiments, a provided oligonucleotide is an altmer. In some embodiments, a provided oligonucleotide is a P-modification altmer. In some embodiments, a provided oligonucleotide is a stereoaltmer. 
     In some embodiments, a provided oligonucleotide is a blockmer. In some embodiments, a provided oligonucleotide is a P-modification blockmer. In some embodiments, a provided oligonucleotide is a stereoblockmer. 
     In some embodiments, a provided oligonucleotide is a gapmer. 
     In some embodiments, a provided oligonucleotide is a skipmer. 
     In some embodiments, a provided oligonucleotide is a combination of one or more of unimer, altmer, blockmer, gapmer, and skipmer. For instance, in some embodiments, a provided oligonucleotide is both an altmer and a gapmer. In some embodiments, a provided nucleotide is both a gapmer and a skipmer. One of skill in the chemical and synthetic arts will recognize that numerous other combinations of patterns are available and are limited only by the commercial availability and/or synthetic accessibility of constituent parts required to synthesize a provided oligonucleotide in accordance with methods of the present invention. 
     In some embodiments, a provided oligonucleotide comprises one or more optionally substituted nucleotides. In some embodiments, a provided oligonucleotide comprises one or more modified nucleotides. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted nucleosides. In some embodiments, a provided oligonucleotide comprises one or more modified nucleosides. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted LNAs. 
     In some embodiments, a provided oligonucleotide comprises one or more optionally substituted nucleobases. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted natural nucleobases. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted modified nucleobases. In some embodiments, a provided oligonucleotide comprises one or more 5-methylcytidine; 5-hydroxymethylcytidine, 5-formylcytosine, or 5-carboxylcytosine. In some embodiments, a provided oligonucleotide comprises one or more 5-methylcytidine. 
     In some embodiments, a provided oligonucleotide comprises one or more optionally substituted sugars. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted sugars found in naturally occurring DNA and RNA. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted ribose or deoxyribose. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted ribose or deoxyribose, wherein one or more hydroxyl groups of the ribose or deoxyribose moiety is optionally and independently replaced by halogen, R′, —N(R′) 2 , —OR′, or —SR′, wherein each R′ is independently as defined above and described herein. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted deoxyribose, wherein the 2′ position of the deoxyribose is optionally and independently substituted with halogen, R′, —N(R′) 2 , —OR′, or —SR′, wherein each R′ is independently as defined above and described herein. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted deoxyribose, wherein the 2′ position of the deoxyribose is optionally and independently substituted with halogen. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted deoxyribose, wherein the 2′ position of the deoxyribose is optionally and independently substituted with one or more —F. halogen. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted deoxyribose, wherein the 2′ position of the deoxyribose is optionally and independently substituted with —OR′, wherein each R′ is independently as defined above and described herein. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted deoxyribose, wherein the 2′ position of the deoxyribose is optionally and independently substituted with —OR′, wherein each R′ is independently an optionally substituted C 1 -C 6  aliphatic. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted deoxyribose, wherein the 2′ position of the deoxyribose is optionally and independently substituted with —OR′, wherein each R′ is independently an optionally substituted C 1 -C 6  alkyl. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted deoxyribose, wherein the 2′ position of the deoxyribose is optionally and independently substituted with —OMe. In some embodiments, a provided oligonucleotide comprises one or more optionally substituted deoxyribose, wherein the 2′ position of the deoxyribose is optionally and independently substituted with —O-methoxyethyl. 
     In some embodiments, a provided oligonucleotide is single-stranded oligonucleotide. 
     In some embodiments, a provided oligonucleotide is a hybridized oligonucleotide strand. In certain embodiments, a provided oligonucleotide is a partially hybridized oligonucleotide strand. In certain embodiments, a provided oligonucleotide is a completely hybridized oligonucleotide strand. In certain embodiments, a provided oligonucleotide is a double-stranded oligonucleotide. In certain embodiments, a provided oligonucleotide is a triple-stranded oligonucleotide (e.g., a triplex). 
     In some embodiments, a provided oligonucleotide is chimeric. For example, in some embodiments, a provided oligonucleotide is DNA-RNA chimera, DNA-LNA chimera, etc. 
     In some embodiments, any one of the structures comprising an oligonucleotide depicted in WO2012/030683 can be modified in accordance with methods of the present invention to provide chirally controlled variants thereof. For example, in some embodiments the chirally controlled variants comprise a stereochemical modification at any one or more of the linkage phosphorus and/or a P-modification at any one or more of the linkage phosphorus. For example, in some embodiments, a particular nucleotide unit of a oligonucleotide of WO2012/030683 is preselected to be stereochemically modified at the linkage phosphorus of that nucleotide unit and/or P-modified at the linkage phosphorus of that nucleotide unit. In some embodiments, a chirally controlled oligonucleotide is of any one of the structures depicted in  FIGS.  26 - 30   . In some embodiments, a chirally controlled oligonucleotide is a variant (e.g., modified version) of any one of the structures depicted in  FIGS.  26 - 30   . The disclosure of WO2012/030683 is herein incorporated by reference in its entirety. 
     In some embodiments, a provided oligonucleotide is a therapeutic agent. 
     In some embodiments, a provided oligonucleotide is an antisense oligonucleotide. 
     In some embodiments, a provided oligonucleotide is an antigene oligonucleotide. 
     In some embodiments, a provided oligonucleotide is a decoy oligonucleotide. 
     In some embodiments, a provided oligonucleotide is part of a DNA vaccine. 
     In some embodiments, a provided oligonucleotide is an immunomodulatory oligonucleotide, e.g., immunostimulatory oligonucleotide and immunoinhibitory oligonucleotide. 
     In some embodiments, a provided oligonucleotide is an adjuvant. 
     In some embodiments, a provided oligonucleotide is an aptamer. 
     In some embodiments, a provided oligonucleotide is a ribozyme. 
     In some embodiments, a provided oligonucleotide is a deoxyribozyme (DNAzymes or DNA enzymes). 
     In some embodiments, a provided oligonucleotide is an siRNA. 
     In some embodiments, a provided oligonucleotide is a microRNA, or miRNA. 
     In some embodiments, a provided oligonucleotide is a ncRNA (non-coding RNAs), including a long non-coding RNA (lncRNA) and a small non-coding RNA, such as piwi-interacting RNA (piRNA). 
     In some embodiments, a provided oligonucleotide is complementary to a structural RNA, e.g., tRNA. 
     In some embodiments, a provided oligonucleotide is a nucleic acid analog, e.g., GNA, LNA, PNA, TNA and Morpholino. 
     In some embodiments, a provided oligonucleotide is a P-modified prodrug. 
     In some embodiments, a provided oligonucleotide is a primer. In some embodiments, a primers is for use in polymerase-based chain reactions (i.e., PCR) to amplify nucleic acids. In some embodiments, a primer is for use in any known variations of PCR, such as reverse transcription PCR (RT-PCR) and real-time PCR. 
     In some embodiments, a provided oligonucleotide is characterized as having the ability to modulate RNase H activation. For example, in some embodiments, RNase H activation is modulated by the presence of stereocontrolled phosphorothioate nucleic acid analogs, with natural DNA/RNA being more or equally susceptible than the Rp stereoisomer, which in turn is more susceptible than the corresponding Sp stereoisomer. 
     In some embodiments, a provided oligonucleotide is characterized as having the ability to indirectly or directly increase or decrease activity of a protein or inhibition or promotion of the expression of a protein. In some embodiments, a provided oligonucleotide is characterized in that it is useful in the control of cell proliferation, viral replication, and/or any other cell signaling process. 
     In some embodiments, a provided oligonucleotide is from about 2 to about 200 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 180 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 160 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 140 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 120 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 100 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 90 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 80 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 70 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 60 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 50 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 40 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 30 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 29 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 28 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 27 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 26 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 25 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 24 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 23 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 22 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 21 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 2 to about 20 nucleotide units in length. 
     In some embodiments, a provided oligonucleotide is from about 4 to about 200 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 180 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 160 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 140 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 120 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 100 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 90 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 80 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 70 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 60 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 50 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 40 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 30 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 29 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 28 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 27 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 26 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 25 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 24 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 23 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 22 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 21 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 4 to about 20 nucleotide units in length. 
     In some embodiments, a provided oligonucleotide is from about 5 to about 10 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 10 to about 30 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 15 to about 25 nucleotide units in length. In some embodiments, a provided oligonucleotide is from about 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 nucleotide units in length. 
     In some embodiments, the oligonucleotide is at least 2 nucleotide units in length. In some embodiments, the oligonucleotide is at least 3 nucleotide units in length. In some embodiments, the oligonucleotide is at least 4 nucleotide units in length. In some embodiments, the oligonucleotide is at least 5 nucleotide units in length. In some embodiments, the oligonucleotide is at least 6 nucleotide units in length. In some embodiments, the oligonucleotide is at least 7 nucleotide units in length. In some embodiments, the oligonucleotide is at least 8 nucleotide units in length. In some embodiments, the oligonucleotide is at least 9 nucleotide units in length. In some embodiments, the oligonucleotide is at least 10 nucleotide units in length. In some embodiments, the oligonucleotide is at least 11 nucleotide units in length. In some embodiments, the oligonucleotide is at least 12 nucleotide units in length. In some embodiments, the oligonucleotide is at least 15 nucleotide units in length. In some embodiments, the oligonucleotide is at least 20 nucleotide units in length. In some embodiments, the oligonucleotide is at least 25 nucleotide units in length. In some other embodiments, the oligonucleotide is at least 30 nucleotide units in length. In some other embodiments, the oligonucleotide is a duplex of complementary strands of at least 18 nucleotide units in length. In some other embodiments, the oligonucleotide is a duplex of complementary strands of at least 21 nucleotide units in length. 
     In some embodiments, the 5′-end and/or the 3′-end of a provided oligonucleotide is modified. In some embodiments, the 5′-end and/or the 3′-end of a provided oligonucleotide is modified with a terminal cap moiety. Exemplary such modifications, including terminal cap moieties are extensively described herein and in the art, for example but not limited to those described in US Patent Application Publication US 2009/0023675A1. 
     Species of Oligonucleotides 
     In certain embodiments, an oligonucleotide of formula I is of any one of the structures shown in Table 2, above and those described in the examples. 
     In some embodiments, a provided chirally controlled oligonucleotide comprises the sequence of, or part of the sequence of mipomersen. Mipomersen is based on the following base sequence GCCT/UCAGT/UCT/UGCT/UT/UCGCACC (SEQ ID NO: 112). In some embodiments, one or more of any of the nucleotide or linkages may be modified in accordance of the present invention. In some embodiments, the present invention provides a chirally controlled oligonucleotide having the sequence of  G *- C *-C*- U *-C*-d A -dG-d T -dC-dT-dG-d mC -dT-dT-dmC-G*-C*-A*-C*-C* (SEQ ID NO: 113) [d=2′-deoxy, *=2′-O-(2-methoxyethyl)] with 3′→5′ phosphorothioate linkages. Exemplary modified mipomersen sequences are described throughout the application, including but not limited to those in Table 4. 
     In certain embodiments, a provided oligonucleotide is a mipomersen unimer. In certain embodiments, a provided oligonucleotide is a mipomersen unimer of configuration Rp. In certain embodiments, a provided oligonucleotide is a mipomersen unimer of configuration Sp. 
     Exemplary chirally controlled oligonucleotides comprising the sequence of, or part of the sequence of mipomersen is depicted in Table 4, below. 
                     TABLE 4                  Exemplary Mipomersen related sequences.                                 SEQ       De-           ID       scrip-       Oligo    NO:   Stereochemistry/Sequence   tion                                     101   106   All-(Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]   All-R               102   106   All-(Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]   All-S               103   106   (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp,   5R-9S-               Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]   5R               104   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp,   5S-9R-               Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]   5S               105   106   (Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Rp, Rp, Rp,   1S-17R-               Rp, Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]   1S               106   106   (Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp,   1R-17S-               Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]   1R               107   106   (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp,   (R/S) 9 R               Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]                   108   106   (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp,   (S/R) 9 S               Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]                   109   106   (Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp,   3S-13R-               Sp)d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]   3S               110   106   (Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp,   3R-13S-               Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]   3R               111   106   (Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp,   18S/R 19                 Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]                   112   106   (Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp,   18S/R 9                 Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]                   113   106   (Sp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp,   18S/R 2                 Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]                   114   106   (Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp,   (RRS) 6 -               Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]   R               115   106   (Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp,   S-(RRS) 6                 Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]                   116   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]   (RRS) 5 -                   RR               122   106   All-(Rp)-   All-R               d[Gs1Cs1Cs1Ts1Cs1As1Gs1Ts1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1Cs1As1Cs1C]                   123   106   (Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Rp, Rp, Rp,   1S-17R-               Rp, Sp)-d[Gs1Cs1Cs1Ts1Cs1As1Gs1Ts1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1Cs1As1Cs1C]   1S               124   106   All-(Sp)-d[Gs1Cs1Cs1Ts1Cs1As1Gs1Ts1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1Cs1As1Cs1C]   All-S               126       All-(Rp)-d[Cs2As2Gs2T]   All-R               127       All-(Rp)-d[Cs3As3Gs3T]   All-R               128       All-(Sp)-d[Cs4As4Gs4T]   All-S               129       All-(Sp)-d[Cs5As5Gs5T]   All-S               130       All-(Sp)-d[Cs6As6Gs6T]   All-S               131   106   All-(Rp)-d[Gs7Cs7Cs7Ts7Cs7As7Gs7Ts7Cs7Ts7Gs7Cs7Ts7Ts7Cs7Gs7Cs7As7Cs7C]   All-R               132   106   All-(Sp)-d[Gs7Cs7Cs7Ts7Cs7As7Gs7Ts7Cs7Ts7Gs7Cs7Ts7Ts7Cs7Gs7Cs7As7Cs7C]   All-S               133   106   (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp,   5R-9S-               Rp)-d[Gs15mCs15mCs1Ts15mCs1As1Gs1Ts15mCs1Ts1   5R               Gs15mCs1Ts1Ts15mCs1Gs15mCs1As15mCs15mC]                   134   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp,   5S-9R-               Sp)-d[Gs15mCs15mCs1Ts15mCs1As1Gs1Ts15mCs1Ts1   5S               Gs15mCs1Ts1Ts15mCs1Gs15mCs1As15mCs15mC]                   135   108   All-(Rp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G]   All-R               136   108   All-(Sp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G]   All-S               137   108   (Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp)-   1S-9R-               d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G]   1S               138   108   (Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp)-   2S-7R-               d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G]   2S               139   108   (Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp)-   1R-9S-               d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G]   1R               140   108   (Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp)-   2R-7S-               d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G]   2R               141   108   (Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp)-   3S-5R-               d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G]   3S               142   108   (Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp)-   3R-5S-               d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G]   3R               143   108   (Sp, Sp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Sp, Sp)-   (SSR) 3 -               d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G]   SS               144   108   (Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp)-   (RRS) 3 -               d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G]   RR               145   109   All-(Rp)-   All-R               d[5mCs1Ts15mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1                   Gs15mC]                   146       All-(Rp)-d[Gs15mCs1Ts1G]   All-R               147       All-(Rp)-d[5mCs1As1Gs1T]   All-R               148   108   All-(Rp)-d[5mCs2As2Gs2Ts25mCs2Ts2Gs25mCs2Ts2Ts25mCs2G]   All-R               149   108   All-(Rp)-d[5mCs4As4Gs4Ts45mCs4Ts4Gs45mCs4Ts4Ts45mCs4G]   All-R               151       All-(Sp)-d[Cs1AsGs1T]   All-S               152       All-(Sp)-d[Cs1AGs1T]   All-S               153       All-(Sp)-d[CAs1GsT]   All-S               157       All-(Sp)-d[5mCs1As1Gs1T]   All-S               158   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCs1GsCsACsC]   4S               159   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp,   5S-9R-               Sp)-d[Gs1Cs1Cs1Ts1CsAsGsTsCsTsGsCsTsTsCs1GsCs2As2Cs2C]   5S               160   106   All-(Rp)-   All-R               (Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) MOE                     161   106   All-(Sp)-   All-S               (Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) MOE                     162   106   (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp,   5R-9S-               Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]   5R               (Gs5mCsAs5mCs5mC) MOE                     163   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp,   5S-9R-               Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]   5S               (Gs5mCsAs5mCs5mC) MOE                     164   106   (Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Rp, Rp, Rp,   1S-17R-               Rp, Sp)-   1S               (Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) MOE                     165   106   (Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp,   1R-17S-               Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]   1R               (Gs5mCsAs5mCs5mC) MOE                     166   106   (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp,   (R/S) 9 R               Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) MOE                     167   106   (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp,   (S/R) 9 S               Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) MOE                     168   106   (Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp,   3S-13R-               Sp)(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]   3S               (Gs5mCsAs5mCs5mC) MOE                     169   106   (Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp,   3R-13S-               Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]   3R               (Gs5mCsAs5mCs5mC) MOE                     170   106   (Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp,   18S/R 19                 Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) MOE                     171   106   (Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp,   18S/R 9                 Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) MOE                     172   106   (Sp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp,   18S/R 2                 Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) MOE                     173   106   (Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp,   (RRS) 6 -R               Rp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) MOE                     174   106   (Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp,   S-(RRS) 6                 Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) MOE                     175   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]   (RRS) 5 -               (Gs5mCsAs5mCs5mC) MOE     RR               176   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)(Gs15mCs15mCs1Ts15mCs1) MOE d[As1Gs1Ts15mCs1Ts1Gs15m   (RRS) 5 -               Cs1Ts1Ts15mCs1] (Gs15mCs1As15mCs15mC) MOE     RR               177   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)(Gs15mCs15mCs1Ts15mCs1) MOE d[AGT5mCTG5mCTT5mC]   (RRS) 5 -               (Gs25mCs2As25mCs25mC) MOE     RR               178   106   (Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp,   S-(RRS) 6                 Sp)-(Gs5mCs5mCsTs5mCs) MOE d[AsGsTs5mCsTsGs5mCsTsTs5mCs]                   (Gs5mCsAs5mCs5mC) F  (F: 2-fluorodeoxyribose)                   179   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)d[Gs8Cs8Cs8Ts8Cs8As8Gs8Ts8Cs8Ts8Gs8Cs8Ts8Ts8Cs8Gs8Cs   (RRS) 5 -               8As8Cs8C]   RR               180   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)d[Gs9Cs9Cs9Ts9Cs9As9Gs9Ts9Cs9Ts9Gs9Cs9Ts9Ts9Cs9Gs9Cs   (RRS) 5 -               9As9Cs9C]   RR               181   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)d[Gs10Cs10Cs10Ts10Cs10As10Gs10Ts10Cs10Ts10Gs10Cs10Ts10   (RRS) 5 -               Ts10Cs10Gs10Cs10As10Cs10C]   RR               182   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)d[Gs11Cs11Cs11Ts11Cs11As11Gs11Ts11Cs11Ts11Gs11Cs11Ts11   (RRS) 5 -               Ts11Cs11Gs11Cs11As11Cs11C]   RR               183   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)d[Gs12Cs12Cs12Ts12Cs12As12Gs12Ts12Cs12Ts12Gs12Cs12Ts12   (RRS) 5 -               Ts12Cs12Gs12Cs12As12Cs12C]   RR               184   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)d[Gs13Cs13Cs13Ts13Cs13As13Gs13Ts13Cs13Ts13Gs13Cs13Ts13   (RRS) 5 -               Ts13Cs13Gs13Cs13As13Cs13C]   RR               185   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)d[Gs14Cs14Cs14Ts14Cs14As14Gs14Ts14Cs14Ts14Gs14Cs14Ts14   (RRS) 5 -               Ts14Cs14Gs14Cs14As14Cs14C]   RR               186   106   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)d[Gs15Cs15Cs15Ts15Cs15As15Gs15Ts15Cs15Ts15Gs15Cs15Ts15   (RRS) 5 -               Ts15Cs15Gs15Cs15As15Cs15C]   RR               187   110   (Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp   RS-               Rp)d[GsCsCs1TsCsAs]GsUs2CsUsGsd[CsTs3TsCsGs]CsAs4CsC   (RRS) 5 -                   RR               188   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsACsC]   4S               189   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[Gs1Cs1Cs1Ts1Cs1As1Gs1Ts1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1CsACs   4S               1C]                   190   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[Gs8Cs8Cs8Ts8Cs8As8Gs8Ts8Cs8Ts8Gs8Cs8Ts8Ts8Cs8Gs8Cs1A   4S               Cs8C]                   191   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[Gs9Cs9Cs9Ts9Cs9As9Gs9Ts9Cs9Ts9Gs9Cs9Ts9Ts9Cs9Gs9Cs1A   4S               Cs9C]                   192   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[Gs10Cs10Cs10Ts10Cs10As10Gs10Ts10Cs10Ts10Gs10Cs10Ts10Ts   4S               10Cs10Gs10Cs1ACs10C]                   193   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[Gs11Cs11Cs11Ts11Cs11As11Gs11Ts11Cs11Ts11Gs11Cs11Ts11Ts   4S               11Cs11Gs11Cs1ACs11C]                   194   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[Gs12Cs12Cs12Ts12Cs12As12Gs12Ts12Cs12Ts12Gs12Cs12Ts12Ts   4S               12Cs12Gs12Cs1ACs12C]                   195   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[Gs13Cs13Cs13Ts13Cs13As13Gs13Ts13Cs13Ts13Gs13Cs13Ts13Ts   4S               13Cs13Gs13Cs1ACs13C]                   196   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[Gs14Cs14Cs14Ts14Cs14As14Gs14Ts14Cs14Ts14Gs14Cs14Ts14Ts   4S               14Cs14Gs14Cs1ACs14C]                   197   106   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               d[Gs15Cs15Cs15Ts15Cs15As15Gs15Ts15Cs15Ts15Gs15Cs15Ts15Ts   4S               15Cs15Gs15Cs1ACs15C]                   198   111   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               GsCsCsUsCsAsGsUsCsUsGsCsUsUsCsGsCsACsC   4S               199   111   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               Gs1Cs1Cs1Us1Cs1As1Gs1Us1Cs1Us1Gs1Cs1Us1Us1Cs1Gs1CsACs   4S               1C                   200   111   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               Gs8Cs8Cs8Us8Cs8As8Gs8Us8Cs8Us8Gs8Cs8Us8Us8Cs8Gs8Cs1AC   4S               s8C                   201   111   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               Gs9Cs9Cs9Us9Cs9As9Gs9Us9Cs9Us9Gs9Cs9Us9Us9Cs9Gs9Cs1AC   4S               s9C                   202   111   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               Gs10Cs10Cs10Us10Cs10As10Gs10Us10Cs10Us10Gs10Cs10Us10Us   4S               10Cs10Gs10Cs1ACs10C                   203   111   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               Gs11Cs11Cs11Us11Cs11As11Gs11Us11Cs11Us11Gs11Cs11Us11Us   4S               11Cs11Gs11Cs1ACs11C                   204   111   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               Gs12Cs12Cs12Us12Cs12As12Gs12Us12Cs12Us12Gs12Cs12Us12Us   4S               12Cs12Gs12Cs1ACs12C                   205   111   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               Gs13Cs13Cs13Us13Cs13As13Gs13Us13Cs13Us13Gs13Cs13Us13Us   4S               13Cs13Gs13Cs1ACs13C                   206   111   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               Gs14Cs14Cs14Us14Cs14As14Gs14Us14Cs14Us14Gs14Cs14Us14Us   4S               14Cs14Gs14Cs1ACs14C                   207   111   (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)-   5S-9R-               Gs15Cs15Cs15Us15Cs15As15Gs15Us15Cs15Us15Gs15Cs15Us15Us   4S               15Cs15Gs15Cs1ACs15C                    
Oligonucleotide Compositions
 
     The present invention provides compositions comprising or consisting of a plurality of provided oligonucleotides (e.g., chirally controlled oligonucleotide compositions). In some embodiments, all such provided oligonucleotides are of the same type, i.e., all have the same base sequence, pattern of backbone linkages (i.e., pattern of internucleotidic linkage types, for example, phosphate, phosphorothioate, etc), pattern of backbone chiral centers (i.e. pattern of linkage phosphorus stereochemistry (Rp/Sp)), and pattern of backbone phosphorus modifications (e.g., pattern of “—XLR 1 ” groups in formula I). In many embodiments, however, provided compositions comprise a plurality of oligonucleotides types, typically in pre-determined relative amounts. 
     In some embodiments, a provided chirally controlled oligonucleotide composition is a chirally pure mipomersen composition. That is to say, in some embodiments, a provided chirally controlled oligonucleotide composition provides mipomersen as a single diastereomer with respect to the configuration of the linkage phosphorus. 
     In some embodiments, a provided chirally controlled oligonucleotide composition is a chirally uniform mipomersen composition. That is to say, in some embodiments, every linkage phosphorus of mipomersen is in the Rp configuration or every linkage phosphorus of mipomersen is in the Sp configuration. 
     In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of one or more provided oligonucleotide types. One of skill in the chemical and medicinal arts will recognize that the selection and amount of each of the one or more types of provided oligonucleotides in a provided composition will depend on the intended use of that composition. That is to say, one of skill in the relevant arts would design a provided chirally controlled oligonucleotide composition such that the amounts and types of provided oligonucleotides contained therein cause the composition as a whole to have certain desirable characteristics (e.g., biologically desirable, therapeutically desirable, etc.). 
     In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of two or more provided oligonucleotide types. In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of three or more provided oligonucleotide types. In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of four or more provided oligonucleotide types. In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of five or more provided oligonucleotide types. In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of six or more provided oligonucleotide types. In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of seven or more provided oligonucleotide types. In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of eight or more provided oligonucleotide types. In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of nine or more provided oligonucleotide types. In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of ten or more provided oligonucleotide types. In some embodiments, a provided chirally controlled oligonucleotide composition comprises a combination of fifteen or more provided oligonucleotide types. 
     In some embodiments, a provided chirally controlled oligonucleotide composition is a combination of an amount of chirally uniform mipomersen of the Rp configuration and an amount of chirally uniform mipomersen of the Sp configuration. 
     In some embodiments, a provided chirally controlled oligonucleotide composition is a combination of an amount of chirally uniform mipomersen of the Rp configuration, an amount of chirally uniform mipomersen of the Sp configuration, and an amount of one or more chirally pure mipomersen of a desired diastereomeric form. 
     Methods for Making Chirally Controlled Oligonucleotides and Compositions Thereof 
     The present invention provides methods for making chirally controlled oligonucleotides and chirally controlled compositions comprising one or more specific nucleotide types. As noted above, the phrase “oligonucleotide type,” as used herein, defines an oligonucleotide that has a particular base sequence, pattern of backbone linkages, pattern of backbone chiral centers, and pattern of backbone phosphorus modifications (e.g., “—XLR 1 ” groups). Oligonucleotides of a common designated “type” are structurally identical to one another with respect to base sequence, pattern of backbone linkages, pattern of backbone chiral centers, and pattern of backbone phosphorus modifications. 
     In some embodiments, a provided chirally controlled oligonucleotide in the invention has properties different from those of the corresponding stereorandom oligonucleotide mixture. In some embodiments, a chirally controlled oligonucleotide has lipophilicity different from that of the stereorandom oligonucleotide mixture. In some embodiments, a chirally controlled oligonucleotide has different retention time on HPLC. In some embodiments, a chirally controlled oligonucleotide may have a peak retention time significantly different from that of the corresponding stereorandom oligonucleotide mixture. During oligonucleotide purification using HPLC as generally practiced in the art, certain chirally controlled oligonucleotides will be largely if not totally lost. During oligonucleotide purification using HPLC as generally practiced in the art, certain chirally controlled oligonucleotides will be largely if not totally lost. One of the consequences is that certain diastereomers of a stereorandom oligonucleotide mixture (certain chirally controlled oligonucleotides) are not tested in assays. Another consequence is that from batches to batches, due to the inevitable instrumental and human errors, the supposedly “pure” stereorandom oligonucleotide will have inconsistent compositions in that diastereomers in the composition, and their relative and absolute amounts, are different from batches to batches. The chirally controlled oligonucleotide and chirally controlled oligonucleotide composition provided in this invention overcome such problems, as a chirally controlled oligonucleotide is synthesized in a chirally controlled fashion as a single diastereomer, and a chirally controlled oligonucleotide composition comprise predetermined levels of one or more individual oligonucleotide types. 
     One of skill in the chemical and synthetic arts will appreciate that synthetic methods of the present invention provide for a degree of control during each step of the synthesis of a provided oligonucleotide such that each nucleotide unit of the oligonucleotide can be designed and/or selected in advance to have a particular stereochemistry at the linkage phosphorus and/or a particular modification at the linkage phosphorus, and/or a particular base, and/or a particular sugar. In some embodiments, a provided oligonucleotide is designed and/or selected in advance to have a particular combination of stereocenters at the linkage phosphorus of the internucleotidic linkage. 
     In some embodiments, a provided oligonucleotide made using methods of the present invention is designed and/or determined to have a particular combination of linkage phosphorus modifications. In some embodiments, a provided oligonucleotide made using methods of the present invention is designed and/or determined to have a particular combination of bases. In some embodiments, a provided oligonucleotide made using methods of the present invention is designed and/or determined to have a particular combination of sugars. In some embodiments, a provided oligonucleotide made using methods of the present invention is designed and/or determined to have a particular combination of one or more of the above structural characteristics. 
     Methods of the present invention exhibit a high degree of chiral control. For instance, methods of the present invention facilitate control of the stereochemical configuration of every single linkage phosphorus within a provided oligonucleotide. In some embodiments, methods of the present invention provide an oligonucleotide comprising one or more modified internucleotidic linkages independently having the structure of formula I. 
     In some embodiments, methods of the present invention provide an oligonucleotide which is a mipomersen unimer. In some embodiments, methods of the present invention provide an oligonucleotide which is a mipomersen unimer of configuration Rp. In some embodiments, methods of the present invention provide an oligonucleotide which is a mipomersen unimer of configuration Sp. 
     In some embodiments, methods of the present invention provide a chirally controlled oligonucleotide composition, i.e., an oligonucleotide composition that contains predetermined levels of individual oligonucleotide types. In some embodiments a chirally controlled oligonucleotide composition comprises one oligonucleotide type. In some embodiments, a chirally controlled oligonucleotide composition comprises more than one oligonucleotide type. In some embodiments, a chirally controlled oligonucleotide composition comprises a plurality of oligonucleotide types. Exemplary chirally controlled oligonucleotide compositions made in accordance with the present invention are described herein. 
     In some embodiments, methods of the present invention provide chirally pure mipomersen compositions with respect to the configuration of the linkage phosphorus. That is to say, in some embodiments, methods of the present invention provide compositions of mipomersen wherein mipomersen exists in the composition in the form of a single diastereomer with respect to the configuration of the linkage phosphorus. 
     In some embodiments, methods of the present invention provide chirally uniform mipomersen compositions with respect to the configuration of the linkage phosphorus. That is to say, in some embodiments, methods of the present invention provide compositions of mipomersen in which all nucleotide units therein have the same stereochemistry with respect to the configuration of the linkage phosphorus, e.g., all nucleotide units are of the Rp configuration at the linkage phosphorus or all nucleotide units are of the Sp configuration at the linkage phosphorus. 
     In some embodiments, a provided chirally controlled oligonucleotide is over 50% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 55% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 60% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 65% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 70% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 75% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 80% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 85% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 90% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 91% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 92% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 93% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 94% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 95% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 96% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 97% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 98% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 99% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 99.5% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 99.6% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 99.7% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 99.8% pure. In some embodiments, a provided chirally controlled oligonucleotide is over about 99.9% pure. In some embodiments, a provided chirally controlled oligonucleotide is over at least about 99% pure. 
     In some embodiments, a chirally controlled oligonucleotide composition is a composition designed to comprise a single oligonucleotide type. In certain embodiments, such compositions are about 50% diastereomerically pure. In some embodiments, such compositions are about 50% diastereomerically pure. In some embodiments, such compositions are about 50% diastereomerically pure. In some embodiments, such compositions are about 55% diastereomerically pure. In some embodiments, such compositions are about 60% diastereomerically pure. In some embodiments, such compositions are about 65% diastereomerically pure. In some embodiments, such compositions are about 70% diastereomerically pure. In some embodiments, such compositions are about 75% diastereomerically pure. In some embodiments, such compositions are about 80% diastereomerically pure. In some embodiments, such compositions are about 85% diastereomerically pure. In some embodiments, such compositions are about 90% diastereomerically pure. In some embodiments, such compositions are about 91% diastereomerically pure. In some embodiments, such compositions are about 92% diastereomerically pure. In some embodiments, such compositions are about 93% diastereomerically pure. In some embodiments, such compositions are about 94% diastereomerically pure. In some embodiments, such compositions are about 95% diastereomerically pure. In some embodiments, such compositions are about 96% diastereomerically pure. In some embodiments, such compositions are about 97% diastereomerically pure. In some embodiments, such compositions are about 98% diastereomerically pure. In some embodiments, such compositions are about 99% diastereomerically pure. In some embodiments, such compositions are about 99.5% diastereomerically pure. In some embodiments, such compositions are about 99.6% diastereomerically pure. In some embodiments, such compositions are about 99.7% diastereomerically pure. In some embodiments, such compositions are about 99.8% diastereomerically pure. In some embodiments, such compositions are about 99.9% diastereomerically pure. In some embodiments, such compositions are at least about 99% diastereomerically pure. 
     In some embodiments, a chirally controlled oligonucleotide composition is a composition designed to comprise multiple oligonucleotide types. In some embodiments, methods of the present invention allow for the generation of a library of chirally controlled oligonucleotides such that a pre-selected amount of any one or more chirally controlled oligonucleotide types can be mixed with any one or more other chirally controlled oligonucleotide types to create a chirally controlled oligonucleotide composition. In some embodiments, the pre-selected amount of an oligonucleotide type is a composition having any one of the above-described diastereomeric purities. 
     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved. 
     When describing the provided methods, the word “cycle” has its ordinary meaning as understood by a person of ordinary skill in the art. In some embodiments, one round of steps (1)-(4) is referred to as a cycle. 
     In some embodiments, the present invention provides methods for making chirally controlled oligonucleotide compositions, comprising steps of: 
     (a) providing an amount of a first chirally controlled oligonucleotide; and 
     (b) optionally providing an amount of one or more additional chirally controlled oligonucleotides. 
     In some embodiments, a first chirally controlled oligonucleotide is an oligonucleotide type, as described herein. In some embodiments, a one or more additional chirally controlled oligonucleotide is a one or more oligonucleotide type, as described herein. 
     One of skill in the relevant chemical and synthetic arts will recognize the degree of versatility and control over structural variation and stereochemical configuration of a provided oligonucleotide when synthesized using methods of the present invention. For instance, after a first cycle is complete, a subsequent cycle can be performed using a nucleotide unit individually selected for that subsequent cycle which, in some embodiments, comprises a nucleobase and/or a sugar that is different from the first cycle nucleobase and/or sugar. Likewise, the chiral auxiliary used in the coupling step of the subsequent cycle can be different from the chiral auxiliary used in the first cycle, such that the second cycle generates a phosphorus linkage of a different stereochemical configuration. In some embodiments, the stereochemistry of the linkage phosphorus in the newly formed internucleotidic linkage is controlled by using stereochemically pure phosphoramidites. Additionally, the modification reagent used in the modifying step of a subsequent cycle can be different from the modification reagent used in the first or former cycle. The cumulative effect of this iterative assembly approach is such that each component of a provided oligonucleotide can be structurally and configurationally tailored to a high degree. An additional advantage to this approach is that the step of capping minimizes the formation of “n−1” impurities that would otherwise make isolation of a provided oligonucleotide extremely challenging, and especially oligonucleotides of longer lengths. 
     In some embodiments, an exemplary cycle of the method for making chirally controlled oligonucleotides is illustrated in Scheme I. In Scheme I, 
                         
represents the solid support, and optionally a portion of the growing chirally controlled oligonucleotide attached to the solid support. The chiral auxiliary exemplified has the structure of formula 3-I:
 
                         
which is further described below. “Cap” is any chemical moiety introduced to the nitrogen atom by the capping step, and in some embodiments, is an amino protecting group. One of ordinary skill in the art understands that in the first cycle, there may be only one nucleoside attached to the solid support when started, and cycle exit can be performed optionally before deblocking. As understood by a person of skill in the art, B PRO  is a protected base used in oligonucleotide synthesis. Each step of the above-depicted cycle of Scheme I is described further below.
 
                         
Synthesis on Solid Support
 
     In some embodiments, the synthesis of a provided oligonucleotide is performed on solid phase. In some embodiments, reactive groups present on a solid support are protected. In some embodiments, reactive groups present on a solid support are unprotected. During oligonucleotide synthesis a solid support is treated with various reagents in several synthesis cycles to achieve the stepwise elongation of a growing oligonucleotide chain with individual nucleotide units. The nucleoside unit at the end of the chain which is directly linked to the solid support is termed “the first nucleoside” as used herein. A first nucleoside is bound to a solid support via a linker moiety, i.e. a diradical with covalent bonds between either of a CPG, a polymer or other solid support and a nucleoside. The linker stays intact during the synthesis cycles performed to assemble the oligonucleotide chain and is cleaved after the chain assembly to liberate the oligonucleotide from the support. 
     Solid supports for solid-phase nucleic acid synthesis include the supports described in, e.g., U.S. Pat. Nos. 4,659,774, 5,141,813, 4,458,066; Caruthers U.S. Pat. Nos. 4,415,732, 4,458,066, 4,500,707, 4,668,777, 4,973,679, and 5,132,418; Andrus et al. U.S. Pat. Nos. 5,047,524, 5,262,530; and Koster U.S. Pat. No. 4,725,677 (reissued as Re34,069). In some embodiments, a solid phase is an organic polymer support. In some embodiments, a solid phase is an inorganic polymer support. In some embodiments, an organic polymer support is polystyrene, aminomethyl polystyrene, a polyethylene glycol-polystyrene graft copolymer, polyacrylamide, polymethacrylate, polyvinylalcohol, highly cross-linked polymer (HCP), or other synthetic polymers, carbohydrates such as cellulose and starch or other polymeric carbohydrates, or other organic polymers and any copolymers, composite materials or combination of the above inorganic or organic materials. In some embodiments, an inorganic polymer support is silica, alumina, controlled polyglass (CPG), which is a silica-gel support, or aminopropyl CPG. Other useful solid supports include fluorous solid supports (see e.g., WO/2005/070859), long chain alkylamine (LCAA) controlled pore glass (CPG) solid supports (see e.g., S. P. Adams, K. S. Kavka, E. J. Wykes, S. B. Holder and G. R. Galluppi,  J. Am. Chem. Soc.,  1983, 105, 661-663; G. R. Gough, M. J. Bruden and P. T. Gilham,  Tetrahedron Lett.,  1981, 22, 4177-4180). Membrane supports and polymeric membranes (see e.g. Innovation and Perspectives in Solid Phase Synthesis, Peptides, Proteins and Nucleic Acids, ch 21 pp 157-162, 1994, Ed. Roger Epton and U.S. Pat. No. 4,923,901) are also useful for the synthesis of nucleic acids. Once formed, a membrane can be chemically functionalized for use in nucleic acid synthesis. In addition to the attachment of a functional group to the membrane, the use of a linker or spacer group attached to the membrane is also used in some embodiments to minimize steric hindrance between the membrane and the synthesized chain. 
     Other suitable solid supports include those generally known in the art to be suitable for use in solid phase methodologies, including, for example, glass sold as Primer™ 200 support, controlled pore glass (CPG), oxalyl-controlled pore glass (see, e.g., Alul, et al.,  Nucleic Acids Research,  1991, 19, 1527), TentaGel Support—an aminopolyethyleneglycol derivatized support (see, e.g., Wright, et al.,  Tetrahedron Lett.,  1993, 34, 3373), and Poros-a copolymer of polystyrene/divinylbenzene. 
     Surface activated polymers have been demonstrated for use in synthesis of natural and modified nucleic acids and proteins on several solid supports mediums. A solid support material can be any polymer suitably uniform in porosity, having sufficient amine content, and sufficient flexibility to undergo any attendant manipulations without losing integrity. Examples of suitable selected materials include nylon, polypropylene, polyester, polytetrafluoroethylene, polystyrene, polycarbonate, and nitrocellulose. Other materials can serve as a solid support, depending on the design of the investigator. In consideration of some designs, for example, a coated metal, in particular gold or platinum can be selected (see e.g., US publication No. 20010055761). In one embodiment of oligonucleotide synthesis, for example, a nucleoside is anchored to a solid support which is functionalized with hydroxyl or amino residues. Alternatively, a solid support is derivatized to provide an acid labile trialkoxytrityl group, such as a trimethoxytrityl group (TMT). Without being bound by theory, it is expected that the presence of a trialkoxytrityl protecting group will permit initial detritylation under conditions commonly used on DNA synthesizers. For a faster release of oligonucleotide material in solution with aqueous ammonia, a diglycolate linker is optionally introduced onto the support. 
     In some embodiments, a provided oligonucleotide alternatively is synthesized from the 5′ to 3′ direction. In some embodiments, a nucleic acid is attached to a solid support through its 5′ end of the growing nucleic acid, thereby presenting its 3′ group for reaction, i.e. using 5′-nucleoside phosphoramidites or in enzymatic reaction (e.g. ligation and polymerization using nucleoside 5′-triphosphates). When considering the 5′ to 3′ synthesis the iterative steps of the present invention remain unchanged (i.e. capping and modification on the chiral phosphorus). 
     Linking Moiety 
     A linking moiety or linker is optionally used to connect a solid support to a compound comprising a free nucleophilic moiety. Suitable linkers are known such as short molecules which serve to connect a solid support to functional groups (e.g., hydroxyl groups) of initial nucleosides molecules in solid phase synthetic techniques. In some embodiments, the linking moiety is a succinamic acid linker, or a succinate linker (—CO—CH 2 —CH 2 —CO—), or an oxalyl linker (—CO—CO—). In some embodiments, the linking moiety and the nucleoside are bonded together through an ester bond. In some embodiments, a linking moiety and a nucleoside are bonded together through an amide bond. In some embodiments, a linking moiety connects a nucleoside to another nucleotide or nucleic acid. Suitable linkers are disclosed in, for example,  Oligonucleotides And Analogues A Practical Approach , Ekstein, F. Ed., IRL Press, N.Y., 1991, Chapter 1 and Solid-Phase Supports for Oligonucleotide Synthesis, Pon, R. T.,  Curr. Prot. Nucleic Acid Chem.,  2000, 3.1.1-3.1.28. 
     A linker moiety is used to connect a compound comprising a free nucleophilic moiety to another nucleoside, nucleotide, or nucleic acid. In some embodiments, a linking moiety is a phosphodiester linkage. In some embodiments, a linking moiety is an H-phosphonate moiety. In some embodiments, a linking moiety is a modified phosphorus linkage as described herein. In some embodiments, a universal linker (UnyLinker) is used to attached the oligonucleotide to the solid support (Ravikumar et al.,  Org. Process Res. Dev.,  2008, 12 (3), 399-410). In some embodiments, other universal linkers are used (Pon, R. T.,  Curr. Prot. Nucleic Acid Chem.,  2000, 3.1.1-3.1.28). In some embodiments, various orthogonal linkers (such as disulfide linkers) are used (Pon, R. T.,  Curr. Prot. Nucleic Acid Chem.,  2000, 3.1.1-3.1.28). 
     General Conditions—Solvents for Synthesis 
     Syntheses of provided oligonucleotides are generally performed in aprotic organic solvents. In some embodiments, a solvent is a nitrile solvent such as, e.g., acetonitrile. In some embodiments, a solvent is a basic amine solvent such as, e.g., pyridine. In some embodiments, a solvent is an ethereal solvent such as, e.g., tetrahydrofuran. In some embodiments, a solvent is a halogenated hydrocarbon such as, e.g., dichloromethane. In some embodiments, a mixture of solvents is used. In certain embodiments a solvent is a mixture of any one or more of the above-described classes of solvents. 
     In some embodiments, when an aprotic organic solvent is not basic, a base is present in the reacting step. In some embodiments where a base is present, the base is an amine base such as, e.g., pyridine, quinoline, or N,N-dimethylaniline. Exemplary other amine bases include pyrrolidine, piperidine, N-methyl pyrrolidine, pyridine, quinoline, N,N-dimethylaminopyridine (DMAP), or N,N-dimethylaniline. 
     In some embodiments, a base is other than an amine base. 
     In some embodiments, an aprotic organic solvent is anhydrous. In some embodiments, an anhydrous aprotic organic solvent is freshly distilled. In some embodiments, a freshly distilled anhydrous aprotic organic solvent is a basic amine solvent such as, e.g., pyridine. In some embodiments, a freshly distilled anhydrous aprotic organic solvent is an ethereal solvent such as, e.g., tetrahydrofuran. In some embodiments, a freshly distilled anhydrous aprotic organic solvent is a nitrile solvent such as, e.g., acetonitrile. 
     Chiral Reagent 
     In provided methods, chiral reagents are used to confer stereoselectivity in the production of chirally controlled oligonucleotides. Many different chiral reagents, also referred to by those of skill in the art and herein as chiral auxiliaries, may be used in accordance with methods of the present invention. Exemplary such chiral reagents are described herein and in Wada I, II and III, referenced above. In certain embodiments, a chiral reagent is as described by Wada I. In some embodiments, a chiral reagent for use in accordance with the methods of the present invention are of Formula 3-I, below: 
                         
wherein W 1  and W 2  are any of —O—, —S—, or —NG 5 -, U 1  and U 3  are carbon atoms which are bonded to U 2  if present, or to each other if r is 0, via a single, double or triple bond. U 2  is —C—, —CG 8 -, —CG 8 G 8 , —NG 8 -, —N—, —O—, or —S— where r is an integer of 0 to 5 and no more than two heteroatoms are adjacent. When any one of U 2  is C, a triple bond must be formed between a second instance of U 2 , which is C, or to one of U 1  or U 3 . Similarly, when any one of U 2  is CG 8 , a double bond is formed between a second instance of U 2  which is —CG 8 - or —N—, or to one of U 1  or U 3 .
 
     In some embodiments, —U 1 —(U 2 ) r —U 3 — is —CG 3 G 4 -CG 1 G 2 -. In some embodiments, —U 1 —(U 2 ) r —U 3 — is —CG 3 =CG 1 -. In some embodiments, —U 1 —(U 2 ) r —U 3 — is —C≡C—. In some embodiments, —U 1 —(U 2 ) r —U 3 — is —CG 3 =CG 8 -CG 1 G 2 -. In some embodiments, —U 1 —(U 2 ) r —U 3 — is —CG 3 G 4 -O—CG 1 G 2 -. In some embodiments, —U 1 —(U 2 ) r —U 3 — is —CG 3 G 4 -NG 8 -CG 1 G 2 -. In some embodiments, —U 1 —(U 2 ) r —U 3 — is —CG 3 G 4 -N—CG 2 -. In some embodiments, —U 1 —(U 2 ) r —U 3 — is —CG 3 G 4 -N═C G 8 -CG 1 G 2 -. 
     As defined herein, G 1 , G 2 , G 3 , G 4 , G 5 , and G 8  are independently hydrogen, or an optionally substituted group selected from alkyl, aralkyl, cycloalkyl, cycloalkylalkyl, heterocyclyl, heteroaryl, and aryl; or two of G 1 , G 2 , G 3 , G 4 , and G 5  are G 6  taken together to form an optionally substituted, saturated, partially unsaturated or unsaturated carbocyclic or heteroatom-containing ring of up to about 20 ring atoms which is monocyclic or polycyclic, and is fused or unfused. In some embodiments, a ring so formed is substituted by oxo, thioxo, alkyl, alkenyl, alkynyl, heteroaryl, or aryl moieties. In some embodiments, when a ring formed by taking two G 6  together is substituted, it is substituted by a moiety which is bulky enough to confer stereoselectivity during the reaction. 
     In some embodiments, a ring formed by taking two of G 6  together is optionally substituted cyclopentyl, pyrrolyl, cyclopropyl, cyclohexenyl, cyclopentenyl, tetrahydropyranyl, or piperazinyl. In some embodiments, a ring formed by taking two of G 6  together is optionally substituted cyclopentyl, pyrrolyl, cyclopropyl, cyclohexenyl, cyclopentenyl, tetrahydropyranyl, pyrrolidinyl, or piperazinyl. 
     In some embodiments, G 1  is optionally substituted phenyl. In some embodiments, G 1  is phenyl. In some embodiments, G 2  is methyl or hydrogen. In some embodiments, G 1  is optionally substituted phenyl and G 2  is methyl. In some embodiments, G 1  is phenyl and G 2  is methyl. 
     In some embodiments, r is 0. 
     In some embodiments, W 1  is —NG 5 -. In some embodiments, one of G 3  and G 4  is taken together with G 5  to form an optionally substituted pyrrolidinyl ring. In some embodiments, one of G 3  and G 4  is taken together with G 5  to form a pyrrolidinyl ring. 
     In some embodiments, W 2  is —O—. 
     In some embodiments, a chiral reagent is a compound of Formula 3-AA: 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments of Formula 3AA, W 1  and W 2  are independently —NG 5 -, —O—, or —S—; G 1 , G 2 , G 3 , G 4 , and G 5  are independently hydrogen, or an optionally substituted group selected from alkyl, aralkyl, cycloalkyl, cycloalkylalkyl, heterocyclyl, heteroaryl, or aryl; or two of G 1 , G 2 , G 3 , G 4 , and G 5  are G 6  taken together to form an optionally substituted saturated, partially unsaturated or unsaturated carbocyclic or heteroatom-containing ring of up to about 20 ring atoms which is monocyclic or polycyclic, fused or unfused, and no more than four of G 1 , G 2 , G 3 , G 4 , and G 5  are G 6 . Similarly to the compounds of Formula 3-I, any of G 1 , G 2 , G 3 , G 4 , or G 5  are optionally substituted by oxo, thioxo, alkyl, alkenyl, alkynyl, heteroaryl, or aryl moieties. In some embodiments, such substitution induces stereoselectivity in chirally controlled oligonucleotide production. 
     In some embodiments, a chiral reagent has one of the following formulae: 
     
       
         
         
             
             
         
       
     
     In some embodiments, a chiral reagent is an aminoalcohol. In some embodiments, a chiral reagent is an aminothiol. In some embodiments, a chiral reagent is an aminophenol. In some embodiments, a chiral reagent is (S)- and (R)-2-methylamino-1-phenylethanol, (1R,2S)-ephedrine, or (1R,2S)-2-methylamino-1,2-diphenylethanol. 
     In some embodiments of the invention, a chiral reagent is a compound of one of the following formulae: 
     
       
         
         
             
             
         
       
     
     The choice of chiral reagent, for example, the isomer represented by Formula Q or its stereoisomer, Formula R, permits specific control of chirality at a linkage phosphorus. Thus, either an Rp or Sp configuration can be selected in each synthetic cycle, permitting control of the overall three dimensional structure of a chirally controlled oligonucleotide. In some embodiments, a chirally controlled oligonucleotide has all Rp stereocenters. In some embodiments of the invention, a chirally controlled oligonucleotide has all Sp stereocenters. In some embodiments of the invention, each linkage phosphorus in the chirally controlled oligonucleotide is independently Rp or Sp. In some embodiments of the invention, each linkage phosphorus in the chirally controlled oligonucleotide is independently Rp or Sp, and at least one is Rp and at least one is Sp. In some embodiments, the selection of Rp and Sp centers is made to confer a specific three dimensional superstructure to a chirally controlled oligonucleotide. Exemplary such selections are described in further detail herein. 
     In some embodiments, a chiral reagent for use in accordance with the present invention is selected for its ability to be removed at a particular step in the above-depicted cycle. For example, in some embodiments it is desirable to remove a chiral reagent during the step of modifying the linkage phosphorus. In some embodiments, it is desirable to remove a chiral reagent before the step of modifying the linkage phosphorus. In some embodiments, it is desirable to remove a chiral reagent after the step of modifying the linkage phosphorus. In some embodiments, it is desirable to remove a chiral reagent after a first coupling step has occurred but before a second coupling step has occurred, such that a chiral reagent is not present on the growing oligonucleotide during the second coupling (and likewise for additional subsequent coupling steps). In some embodiments, a chiral reagent is removed during the “deblock” reaction that occurs after modification of the linkage phosphorus but before a subsequent cycle begins. Exemplary methods and reagents for removal are described herein. 
     In some embodiments, removal of chiral auxiliary is achieved when performing the modification and/or deblocking step, as illustrated in Scheme I. It can be beneficial to combine chiral auxiliary removal together with other transformations, such as modification and deblocking. A person of ordinary skill in the art would appreciate that the saved steps/transformation could improve the overall efficiency of synthesis, for instance, with respect to yield and product purity, especially for longer oligonucleotides. One example wherein the chiral auxiliary is removed during modification and/or deblocking is illustrated in Scheme I. 
     In some embodiments, a chiral reagent for use in accordance with methods of the present invention is characterized in that it is removable under certain conditions. For instance, in some embodiments, a chiral reagent is selected for its ability to be removed under acidic conditions. In certain embodiments, a chiral reagent is selected for its ability to be removed under mildly acidic conditions. In certain embodiments, a chiral reagent is selected for its ability to be removed by way of an E1 elimination reaction (e.g., removal occurs due to the formation of a cation intermediate on the chiral reagent under acidic conditions, causing the chiral reagent to cleave from the oligonucleotide). In some embodiments, a chiral reagent is characterized in that it has a structure recognized as being able to accommodate or facilitate an E1 elimination reaction. One of skill in the relevant arts will appreciate which structures would be envisaged as being prone toward undergoing such elimination reactions. 
     In some embodiments, a chiral reagent is selected for its ability to be removed with a nucleophile. In some embodiments, a chiral reagent is selected for its ability to be removed with an amine nucleophile. In some embodiments, a chiral reagent is selected for its ability to be removed with a nucleophile other than an amine. 
     In some embodiments, a chiral reagent is selected for its ability to be removed with a base. In some embodiments, a chiral reagent is selected for its ability to be removed with an amine. In some embodiments, a chiral reagent is selected for its ability to be removed with a base other than an amine. 
     Further Embodiments of Chiral Reagents 
     In some embodiments, the present invention is directed to a chiral reagent that is used to synthesize chirally controlled oligonucleotides. 
     In some embodiments, the present invention provides chiral reagents that are stable to the coupling, capping, modifying and deblocking steps described above and herein. In some embodiments, the present invention provides chiral reagents that are stable to the modifying and deblocking steps described above and herein. In some embodiments, the present invention provides chiral reagents that are stable to the sulfurization and deblocking steps described above and herein. In some embodiments, the present invention provides chiral reagents that are stable to the oxidation step described above and herein. In some embodiments, such a chiral reagent has a structure of formula Z-I. 
     In some embodiments, the present invention provides chiral reagents that are removed by treatment with a base and/or a nucleophile. In some embodiments, the present invention provides chiral reagents that are removed by treatment with a base and/or a nucleophile, and are stable to the coupling, capping, modifying and deblocking steps described above and herein. In some embodiments, the present invention provides chiral reagents that are removed by treatment comprising an amine. In some embodiments, the present invention provides chiral reagents that are removed by treatment comprising an amine, and are stable to the coupling, capping, modifying and deblocking steps described above and herein. In some embodiments, the present invention provides chiral reagents that are removed by the deprotection/cleavage conditions described in this application, and are stable to the coupling, capping, modifying and deblocking steps described above and herein. In some embodiments, such a chiral reagent has a structure of formula Z-I. 
     In some embodiments, the chiral reagents that are stable to the coupling, capping, modifying and deblocking steps are used to synthesize chirally controlled oligonucleotides described above and herein. In some embodiments, the chiral reagents that are stable to the coupling, capping, modifying and deblocking steps are used to synthesize chirally controlled oligonucleotides described above and herein, wherein the chirally controlled oligonucleotides comprise one or more phosphate diester or phosphorothioate diester linkages. In some embodiments, the chiral reagents that are stable to the coupling, capping, modifying and deblocking steps are used to synthesize chirally controlled oligonucleotides comprising one or more phosphate diester or phosphorothioate diester linkages, and are not removed until the desired oligonucleotide lengths have been achieved. In some embodiments, the chiral reagents that are stable to the coupling, capping, modifying and deblocking steps are used to synthesize chirally controlled oligonucleotides comprising one or more phosphate diester or phosphorothioate diester linkages, and are not removed until after cycle exit. In some embodiments, the chiral reagents that are stable to the coupling, capping, modifying and deblocking steps are used to synthesize chirally controlled oligonucleotides comprising one or more phosphate diester or phosphorothioate diester linkages, and are not removed until cleavage from solid support. In some embodiments, the chiral reagents that are stable to the coupling, capping, modifying and deblocking steps are used to synthesize chirally controlled oligonucleotides comprising one or more phosphate diester or phosphorothioate diester linkages, and are not removed until cleavage from solid support, and the removal is performed in the same step as cleavage from solid support. In some embodiments, such a chiral reagent has a structure of formula Z-I. 
     In some embodiments, when a chiral reagent that is stable to the coupling, capping, modifying and deblocking steps is used in oligonucleotide synthesis, the oligonucleotide with 5′-OH ready for coupling can be from any synthetic cycle, including those described in Schemes I, I-b, I-c, I-d, Z-1 and Z-2. In some embodiments, the oligonucleotide with 5′-OH for coupling comprises various types of internucleotidic linkages as described above and herein. After coupling, the modifying step as described in this application installs the desired modification to the linkage phosphorus. The product can either go to cycle exit before/after deblocking, or enter the next cycle after deblocking the 5′-OH. It is understood by a person of ordinary skill in the art that the next cycle can be any of the synthetic cycles described in this application, including but not limited to those in Schemes I, I-b, I-c, I-d, Z-1 and Z-2. 
     In some embodiments, a chiral reagent or a salt thereof for use in accordance with the present invention is of chemical formula (Z-I). 
     
       
         
         
             
             
         
       
     
     In the formula (Z-I), G z1  and G z2  are independently a hydrogen atom, a nitro group (—NO 2 ), a halogen atom, a cyano group (—CN), a group of formula (Z-II) or (Z-III), or both G 1  and G 2  taken together to form a group of formula (Z-IV). 
     In some embodiments, a group of formula (Z-II) is as depicted below: 
                         
wherein G 21  to G 23  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group.
 
     In some embodiments, a group of formula (Z-III) is as depicted below: 
                         
wherein G 31  to G 33  are independently C 1-4  alkyl group, C 6-14  aryl group C 1-4  alkoxy group, C 7-14  aralkyl group, C 1-4  alkyl C 6-14  aryl group, C 1-4  alkoxy C 6-14  aryl group, or C 6-14  aryl C 1-4  alkyl group.
 
     In some embodiments, a group of formula (Z-IV) is as depicted below: 
                         
wherein G 41  to G 46  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group.
 
     G z3  and G z4  are independently a hydrogen atom, C 1-3  alkyl group, C 6-14  aryl group, or both G z3  and G z4  taken together to form a heteroatom-containing ring that has 3 to 16 carbon atoms, together with the NH moiety in formula (Z-I). 
     In some embodiments, a chiral reagent has following chemical formula (Z-I′): 
                         
wherein G z1  and G z2  are same as above. Namely, G z1  and G z2  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group, a group of formula (Z-II) or (Z-III), or both G z1  and G z2  taken together to form a group of formula (Z-IV).
 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and each of G z1  and G z2  is a group of formula (Z-II), wherein G 21  to G 23  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and each of G z1  and G z2  is a group of formula (Z-II) and each of G 21  to G 23  is a hydrogen atom. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-II), and G 21  to G 23  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-II), each of G 21  and G 22  is a hydrogen atom and G 23  is a nitro group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom and G z2  is a group of formula (III), and G 31  to G 33  are independently C 1-4  alkyl group, C 6-14  aryl group, C 7-14  aralkyl group, C 1-4  alkyl C 6-14  aryl group, C 1-4  alkoxy C 6-14  aryl group, or C 6-14  aryl C 1-4  alkyl group. 
     In some embodiments, the chiral reagent has chemical formula (I′) and G 1  is a hydrogen atom and G 2  is a group of formula (III), and G 31  to G 33  are independently C 1-4  alkyl group, C 6  aryl group, C 7-10  aralkyl group, C 1-4  alkyl C 6  aryl group, C 1-4  alkoxy C 6  aryl group, or C 6  aryl C 1-4  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-III), and G 31  to G 33  are independently C 1-4  alkyl group or C 6  aryl group. Examples of C 1-4  alkyl group are methyl group, ethyl group, n-propyl group, iso-propyl group, n-buthyl group and tert-buthyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-III), and G 31  to G 33  are independently C 1-4  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-III), and G 31  and G 33  are C 6  aryl group and G 32  is C 1-4  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  and G z2  are taken together to form a group of formula (Z-IV), and G 41  to G 46  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-4  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  and G z2  are taken together to form a group of formula (Z-IV), wherein each of G 41  to G 46  is a hydrogen atom. 
     In certain embodiments, a chiral reagent is selected from one of chemical formulae 3a, 3b, 5a, Z-5b, 7a, 7b, 9a, 9b, 11a and 11b: 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     In some embodiments, a nucleoside 3′-phosphoramidite derivative for use in accordance with the present invention is represented by formula (Z-Va) or (Z-Vb): 
                         
wherein G z1  to G z4  are the same as above, G z5  is a protective group of the hydroxyl group, and Bs is a group selected from the groups represented by following formula (Z-VI) to (Z-XI), or derivatives thereof.
 
                         
Examples of Bs are an adenine, a thymine, a cytosine, a guanine, an uracil, a 5-methylcytosine or derivative thereof;
 
R z2  is independently hydrogen, —OH, —SH, —NR d R d , —N 3 , halogen, alkyl, alkenyl, alkynyl, alkyl-Y 1 —, alkenyl-Y 1 —, alkynyl-Y 1 —, aryl-Y 1 —, heteroaryl-Y 1 —, —OR b , or —SR b , wherein R b  is a blocking moiety;
 
Y 1  is O, NR d , S, or Se;
 
R d  is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, —P(O)(R e ) 2 , or —HP(O)(R e );
 
R e  is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y 2 —, alkenyl-Y 2 —, alkynyl-Y 2 —, aryl-Y 2 —, or heteroaryl-Y 2 —, or a cation which is Na + , Li + , or K + , or —O − ;
 
Y 2  is O, NR d , or S;
 
R z3  is a group represented by —CH 2 —, —(CH 2 ) 2 —, —CH 2 NH—, or —CH 2 N(CH 3 )—.
 
     Examples of G 5  are trityl, 4-monomethoxytrityl, 4,4′-dimethoxytrityl, 4,4′,4″-trimethoxytrityl, 9-phenylxanthin-9-yl (Pixyl) and 9-(p-methoxyphenyl)xanthin-9-yl (MOX). 
     In some embodiments, a nucleoside 3′-phosphoramidite derivative is represented by formula (Z-Va′) or (Z-Vb′): 
                         
wherein each of G z1 , G z2 , G z5 , Bs, R z2 , and R z3  is independently as defined above and described herein.
 
     In some embodiments, the invention relates to a method for synthesis of a chirally controlled oligonucleotide. 
     In some embodiments, a provided method comprises a first step of reacting a molecule comprising an achiral H-phosphonate moiety, the first activating reagent and a chiral reagent or a salt thereof to form a monomer. In some embodiments, a chiral reagent has chemical formula (Z-I) and the monomer may be represented by formula (Z-Va), (Z-Vb), (Z-Va′), or (Z-Vb′). The monomer reacts with the second activating reagent and a nucleoside to form a condensed intermediate. In some embodiments, a subsequent step comprises converting the condensed intermediate to the nucleic acid comprising a chiral X-phosphonate moiety. 
     In some embodiments, the present methods provide stable and commercially available materials as starting materials. In some embodiments, the present methods provide a stereocontrolled phosphorous atom-modified oligonucleotide using an achiral starting material. 
     As shown in the working examples, in some embodiments methods of the present invention do not cause degradation during deprotection steps. Further the method does not require special capping agents to produce phosphorus atom-modified oligonucleotide derivatives. 
     In some embodiments, the present invention provides a method for synthesis of stereocontrolled phosphorus atom-modified oligonucleotide derivatives using a chiral monomer. In some embodiments, the first step is reacting a nucleoside 3′-phosphoramidite derivative which is represented by formula (Z-Va), (Z-Vb), (Z-Va′), or (Z-Vb′) with the second activating reagent and a nucleoside to form a condensed intermediate. The second step is converting the condensed intermediate to the nucleic acid comprising a chiral X-phosphonate moiety. 
     All publications and patent applications disclosed herein in this specification are herein incorporated by reference in their entirety to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. 
     As used in this “Further Embodiments of Chiral Reagents” section, in a condensation reaction, the term “activating reagent” refers to a reagent that activates a less reactive site and renders it more susceptible to attack by a nucleophile. 
     As used in this “Further Embodiments of Chiral Reagents” section, an “alkyl” group refers to an aliphatic hydrocarbon group. The alkyl moiety may be a saturated alkyl group (which means that it does not contain any units of unsaturation, e.g. carbon-carbon double bonds or carbon-carbon triple bonds) or the alkyl moiety may be an unsaturated alkyl group (which means that it contains at least one unit of unsaturation). The alkyl moiety, whether saturated or unsaturated, may be branched, straight chain, or include a cyclic portion. The point of attachment of an alkyl is at a carbon atom that is not part of a ring. The “alkyl” moiety may have 1 to 10 carbon atoms (whenever it appears herein, a numerical range such as “1 to 10” refers to each integer in the given range; e.g., “1 to 10 carbon atoms” means that the alkyl group may consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, etc., up to and including 10 carbon atoms, although the present definition also covers the occurrence of the term “alkyl” where no numerical range is designated). Alkyl includes both branched and straight chain alkyl groups. The alkyl group of the compounds described herein may be designated as “C 1 -C 6  alkyl” or similar designations. By way of example only, “C 1 -C 6  alkyl” indicates that there are one, two, three, four, five, or six carbon atoms in the alkyl chain, i.e., the alkyl chain is selected from e.g., methyl, ethyl, propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, and tert-butyl. Typical alkyl groups include, but are in no way limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tertiary butyl, pentyl, hexyl, allyl, cyclopropylmethyl, cyclobutylmethyl, cyclopentylmethyl, cyclohexylmethyl, and the like. In one aspect, an alkyl is a C 1 -C 6  alkyl. C 1-3  alkyl group means straight or branched alkyl group that has 1 to 3 carbon atoms. Examples of C 1-3  alkyl group are methyl, ethyl, propyl and isopropyl. C 1-4  alkyl group means straight or branched alkyl group that has 1 to 4 carbon atoms. Examples of C 1-4  alkyl group are methyl, ethyl, propyl, isopropyl, butyl, isobutyl, and tert-butyl. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “aryl” refers to an aromatic ring wherein each of the atoms forming the ring is a carbon atom. Aryl rings are formed by five, six, seven, eight, nine, or more than nine carbon atoms. Aryl groups are a substituted or unsubstituted. In one aspect, an aryl is a phenyl or a naphthalenyl. Depending on the structure, an aryl group can be a monoradical or a diradical (i.e., an arylene group). In one aspect, an aryl is a C 6 -C 10  aryl. C 6-14  aryl group means aryl group that has 6 to 14 carbon atoms. The examples of C 6-14  aryl group are phenyl, biphenyl, naphthyl, anthracyl, indanyl, phthalimidyl, naphthimidyl, phenanthridinyl, and tetrahydronaphthyl. 
     The term “aralkyl” refers to an alkyl group substituted with an aryl group. Suitable aralkyl groups include benzyl, picolyl, and the like, all of which may be optionally substituted. 
     As used in this “Further Embodiments of Chiral Reagents” section, An “acyl moiety” refers to an alkyl(C═O), aryl(C═O), or aralkyl(C═O) group. An acyl moiety can have an intervening moiety (Y) that is oxy, amino, thio, or seleno between the carbonyl and the hydrocarbon group. For example, an acyl group can be alkyl-Y—(C═O), aryl-Y—(C═O) or aralkyl-Y—(C═O). 
     As used in this “Further Embodiments of Chiral Reagents” section, “alkenyl” groups are straight chain, branch chain, and cyclic hydrocarbon groups containing at least one carbon-carbon double bond. Alkenyl groups can be substituted. 
     As used in this “Further Embodiments of Chiral Reagents” section, “alkynyl” groups are straight chain, branch chain, and cyclic hydrocarbon groups containing at least one carbon-carbon triple bond. Alkynyl groups can be substituted. 
     As used in this “Further Embodiments of Chiral Reagents” section, an “alkoxy” group refers to an alklyl group linked to oxygen i.e. (alkyl)-O— group, where alkyl is as defined herein. Examples include methoxy (—OCH3) or ethoxy (—OCH2CH3) groups. 
     As used in this “Further Embodiments of Chiral Reagents” section, an “alkenyloxy” group refers to an alkenyl group linked to oxygen i.e. (alkenyl)-O— group, where alkenyl is as defined herein. 
     As used in this “Further Embodiments of Chiral Reagents” section, an “alkynyloxy” group refers to an alkynyl group linked to oxygen i.e. (alkynyl)-O— group, where alkynyl is as defined herein. 
     As used in this “Further Embodiments of Chiral Reagents” section, an “aryloxy” group refers to an aryl group linked to oxygen i.e. (aryl)-O-group, where the aryl is as defined herein. An example includes phenoxy (—OC 6 H 5 ) group. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “alkylseleno” refers to an alkyl group having a substituted seleno group attached thereto i.e. (alkyl)-Se— group, wherein alkyl is defined herein. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “alkenylseleno” refers to an alkenyl group having a substituted seleno group attached thereto i.e. (alkenyl)-Se— group, wherein alkenyl is defined herein. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “alkynylseleno” refers to an alkynyl group having a substituted seleno group attached thereto i.e. (alkynyl)-Se— group, wherein alkenyl is defined herein. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “alkylthio” refers to an alkyl group attached to a bridging sulfur atom i.e. (alkyl)-S-group, wherein alkyl is defined herein. For example, an alkylthio is a methylthio and the like. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “alkenylthio” refers to an alkenyl group attached to a bridging sulfur atom i.e. (alkenyl)-S— group, wherein alkenyl is defined herein. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “alkynylthio” refers to an alkynyl group attached to a bridging sulfur atom i.e. (alkynyl)-S— group, wherein alkenyl is defined herein. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “alkylamino” refers to an amino group substituted with at least one alkyl group i.e. —NH(alkyl) or —N(alkyl)2, wherein alkyl is defined herein. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “alkenylamino” refers to an amino group substituted with at least one alkenyl group Le —NH(alkenyl) or —N(alkenyl)2, wherein alkenyl is defined herein. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “alkynylamino” refers to an amino group substituted with at least one alkynyl group i.e. —NH(alkynyl) or —N(alkynyl) 2 , wherein alkynyl is defined herein. 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “halogen” is intended to include fluorine, chlorine, bromine and iodine. 
     As used in this “Further Embodiments of Chiral Reagents” section, a “fluorescent group” refers to a molecule that, when excited with light having a selected wavelength, emits light of a different wavelength. Fluorescent groups include, but are not limited to, indole groups, fluorescein, tetramethylrhodamine, Texas Red, BODIPY, 5-[(2-aminoethyl)amino]napthalene-1-sulfonic acid (EDANS), coumarin and Lucifer yellow. 
     As used in this “Further Embodiments of Chiral Reagents” section, an “ammonium ion” is a positively charged polyatomic cation of the chemical formula NH 4   + . 
     As used in this “Further Embodiments of Chiral Reagents” section, an “alkylammonium ion” is an ammonium ion that has at least one of its hydrogen atoms replaced by an alkyl group, wherein alkyl is defined herein. Examples include triethylammonium ion, N, N-diisopropylethylammonium ion. 
     As used in this “Further Embodiments of Chiral Reagents” section, an “iminium ion” has the general structure (R x ) 2 C═N(R x ) 2   +  The R x  groups refer to alkyl, alkenyl, alkynyl, aryl groups as defined herein. A “heteroaromatic iminium ion” refers to an imminium ion where the nitrogen and its attached R x  groups form a heteroaromatic ring. A “heterocyclic iminium ion” refers to an imminium ion where the nitrogen and its attached R x  groups form a heterocyclic ring. 
     As used in this “Further Embodiments of Chiral Reagents” section, the terms “amino” or “amine” refers to a —N(R h ) 2  radical group, where each R h  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl, unless stated otherwise specifically in the specification. When a —N(R h ) 2  group has two R h  other than hydrogen they can be combined with the nitrogen atom to form a 4-, 5-, 6-, or 7-membered ring. For example, —N(R h ) 2  is meant to include, but not be limited to, 1-pyrrolidinyl and 4-morpholinyl. Any one or more of the hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl are optionally substituted by one or more substituents which independently are alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilyl, —OR i , —SR i , —OC(O)R i , —N(R i ) 2 , —C(O)R i , —C(O)OR i , —OC(O)N(R i ) 2 , —C(O)N(R i ) 2 , —N(R i )C(O)OR, —N(R i )C(O)R i , —N(R i )C(O)N(R i ) 2 , N(R i )C(NR)N(R i ) 2 , —N(R i )S(O) t R i  (where t is 1 or 2), —S(O), or —S(O) t N(R i ) 2  (where t is 1 or 2), where each R i  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl. 
     As used in this “Further Embodiments of Chiral Reagents” section, “carbamate” as used herein, refers to a moiety attached to an amino group which has the formula —C(O)OR where R is alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl. Examples include but are not limited to Boc (tert-butyl-OC(O)—), CBz (benzyl-OC(O)—), Teoc (Me 3 SiCH 2 CH 2 OC(O)—), alloc (allyl-OC(O)—), or Fmoc (9-fluorenylmethyl-OC(O)—) group 
     As used in this “Further Embodiments of Chiral Reagents” section, “substituted silyl” as used herein, refers to a moiety which has the formula R x   3 Si—. Examples include, but are not limited to, TBDMS (tert-butyldimethylsilyl), TBDPS (tert-butyldiphenylsilyl) or TMS (trimethylsilyl). 
     As used in this “Further Embodiments of Chiral Reagents” section, the term “thiol” refers to —SH groups, and include substituted thiol groups i.e. —SRJ groups, wherein RJ are each independently a substituted or unsubstituted alkyl, cycloalkyl, alkenyl, alkynyl, aryl aralkyl, heterocyclyl or heterocyclylalkyl group as defined herein. 
     In some embodiments, the present invention provides a chiral reagent or a salt thereof. In some embodiments, a chiral reagent is of the following chemical formula (Z-I): 
                         
wherein G z1  and G z2  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group (—CN), a group of formula (Z-II) or (Z-III), or both G z1  and G z2  taken together to form a group of formula (Z-IV). In some embodiments, the term “chiral reagent” is a chemical composition which is used to produce stereocontrolled phosphorous atom-modified nucleotide or oligonucleotide derivatives. A chiral reagent reacts with a nucleoside to form a chiral intermediate.
 
     In some embodiments, a group of formula (Z-II) is of the following formula: 
                         
wherein G 21  to G 23  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. In some embodiments, examples of G21 to G23 are a hydrogen atom.
 
     In some embodiments, a group of formula (Z-III) is of the following formula: 
                         
wherein G 31  to G 33  are independently C 1-4  alkyl group, C 6-14  aryl group, C 1-4  alkoxy group, C 7-14  aralkyl group, C 1-4  alkyl C 6-14  aryl group, C 1-4  alkoxy C 6-14  aryl group, or C 6-14  aryl C 1-4  alkyl group. Examples of C 1-4  alkyl C 6-14  aryl group are methylphenyl group, and ethylphenyl group. Examples of C 1-4  alkoxy C 6-14  aryl group are methoxyphenyl group and ethoxyphenyl group. Examples of C 6-14  aryl C 1-4  alkyl groups are benzyl group and phenylethyl group. In some embodiments, examples of G 31  to G 33  are independently a methyl group and a phenyl group.
 
     In some embodiments, a group of formula (Z-IV) is of the following formula: 
                         
wherein G 41  to G 46  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. In some embodiments, examples of G41 to G46 are a hydrogen atom.
 
     G z3  and G z4  are independently a hydrogen atom, C 1-3  alkyl group, C 6-14  aryl group, or both G z3  and G z4  taken together to form a heteroatom-containing ring that has 3 to 16 carbon atoms. In some embodiments, examples of G 3  and G 4  are that taken together to form a heteroatom-containing ring that has 3 to 16 carbon atoms with NH moiety in the formula (I). 
     In certain embodiments, a chiral reagent has following chemical formula (Z-I′). 
     
       
         
         
             
             
         
       
     
     In the formula (Z-I′), G z1  and G z2  are same as above and G z1  and G z2  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group, a group of formula (Z-II) or (Z-III), or both G z1  and G z2  taken together to form a group of formula (Z-IV). 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and each of G z1  and G z2  is a group of formula (Z-II), wherein G 21  to G 23  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and each of G z1  and G z2  is a group of formula (Z-II) and each of G 21  to G 23  is a hydrogen atom. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-II), and G 21  to G 23  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-II), each of G 21  and G 22  is a hydrogen atom and G 23  is a nitro group (—NO 2 ). 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom and G z2  is a group of formula (Z-III), and G 31  to G 33  are independently C 1-4  alkyl group, C 6-14  aryl group, C 7-14  aralkyl group, C 1-4  alkyl C 6-14  aryl group, C 1-4  alkoxy C 6-14  aryl group, or C 6-14  aryl C 1-4  alkyl group. 
     In some embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-III), and G 31  to G 33  are independently C 1-4  alkyl group or C 6  aryl group (a phenyl group). Examples of C 1-4  alkyl group are methyl group, ethyl group, n-propyl group, iso-propyl group, n-butyl group and tert-butyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-III), and G 31  to G 33  are independently C 1-2  alkyl group (a methyl group or an ethyl group) or C 6  aryl group (a phenyl group). 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-III), and G 31  to G 33  are independently C 1-4  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  is a hydrogen atom, G z2  is a group of formula (Z-III), and G 31  and G 33  are C 6  aryl group (a phenyl group) and G 32  is C 1-2  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  and G z2  are taken together to form a group of formula (Z-IV), and G 41  to G 46  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. 
     In certain embodiments, a chiral reagent has chemical formula (Z-I′) and G z1  and G z2  are taken together to form a group of formula (Z-IV), wherein each of G 41  to G 46  is a hydrogen atom. 
     In certain embodiments, a chiral reagent is selected from one of chemical formulae 3a, 3b, 5a, Z-5b, 7a, 7b, 9a, 9b, 11a and 11b: 
                                           
Namely, in some embodiments, a chiral reagent is selected from:
     (S)-2-(Methyldiphenylsilyl)-1-((S)-1-pyrrolidin-2-yl)ethanol (3a),   (R)-2-(Methyldiphenylsilyl)-1-((R)-1-pyrrolidin-2-yl)ethanol (3b),   (S)-2-(Trimethylsilyl)-1-((S)-1-pyrrolidin-2-yl)ethanol (5a),   (R)-2-(Trimethylsilyl)-1-((R)-1-pyrrolidin-2-yl)ethanol (Z-5b),   (R)-2,2-Diphenyl-1-((S)-pyrrolidin-2-yl)ethanol (7a),   (S)-2,2-Diphenyl-1-((R)-pyrrolidin-2-yl)ethanol (7b),   (R)-2-(4-Nitrophenyl)-1-((S)-pyrrolidin-2-yl)ethanol (9a),   (S)-2-(4-Nitrophenyl)-1-((R)-pyrrolidin-2-yl)ethanol (9b),   (R)-(9H-Fluororen-9-yl)((S)-pyrrolidin-2-yl)methanol (11a), or   (S)-(9H-Fluororen-9-yl)((R)-pyrrolidin-2-yl)methanol (11b).   

     The chiral reagent reacts with a nucleic acid or modified nucleic acid to be an asymmetric auxiliary group. A nucleoside 3′-phosphoramidite derivative, which is an intermediate of manufacturing a stereocontrolled phosphorous atom-modified oligonucleotide derivative, is obtained by chiral reagent reacting with a nucleic acid or modified nucleic acid. 
     In some embodiments, the invention provides a nucleoside 3′-phosphoramidite derivative which is represented by formula (Z-Va) or (Z-Vb). The compounds of formula (Z-Va) and (Z-Vb) are known as monomers that are used in synthesizing oligonucleotide derivatives. These compounds are also known as oxazaphospholidine monomers. The sugar moieties of the compounds represented by formula (Z-Vb) are known as BNA and LNA (when R z3  is a methylene group). 
     
       
         
         
             
             
         
       
     
     In the formula (Z-Va) and (Z-Va), G z1  to G z4  are same as above, G z5  is a protective group of the hydroxyl group, and Bs is a group selected from the groups represented by formula (Z-VI) to (Z-XI) or derivatives thereof. 
                         
Examples of Bs are an adenine, a thymine, a cytosine, a guanine, an uracil, a 5-methylcytosine or derivative thereof;
 
R z2  is independently hydrogen, —OH, —SH, —NR d R d , —N 3 , halogen, alkyl, alkenyl, alkynyl, alkyl-Y 1 —, alkenyl-Y 1 —, alkynyl-Y 1 —, aryl-Y 1 —, heteroaryl-Y 1 —, —OR b , or —SR b , wherein R b  is a blocking moiety;
 
Y 1  is O, NR d , S, or Se;
 
R d  is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, —P(O)(R e ) 2 , or —HP(O)(R e );
 
R e  is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y 2 —, alkenyl-Y 2 —, alkynyl-Y 2 —, aryl-Y 2 —, or heteroaryl-Y 2 —, or a cation which is Na + , Li + , or K + , or —O − ;
 
Y 2  is O, NR d , or S;
 
R z3  is a group represented by —CH 2 —, —(CH 2 ) 2 —, —CH 2 NH—, or —CH 2 N(CH 3 )—.
 
     Examples of G z5  is trityl, 4-monomethoxytrityl, 4,4′-dimethoxytrityl, 4,4′,4″-trimethoxytrityl, 9-phenylxanthin-9-yl (Pixyl) and 9-(p-methoxyphenyl)xanthin-9-yl (MOX). 
     In some embodiments, Bs is an adenine, a thymine, a cytosine, a guanine, or derivative thereof. In some embodiments, Bs is a nucleobase or a modified nucleobase. Exemplary derivatives are, for instance, those disclosed in JP 2005-89441 A, and are represented as follows: 
                         
wherein, in the above formula, each of R 8  to R 10  is independently C 1-10  alkyl, C 6 -C 10  aryl, C 6 -C 1  aralkyl, or C 6 -C 10  aryloxyalkyl. In some embodiments, R 8  is methyl, isopropyl, phenyl, benzyl, and phenoxymethyl. In some embodiments, R 9  and R 10  are C 1-4  alkyl group.
 
     In some embodiments, a nucleoside 3′-phosphoramidite derivative is represented by formula (Z-Va′) or (Z-Vb′): 
                         
wherein, in the formula (Z-Va′) and (Z-Vb′), each of G z1 , G z2 , G z5 , Bs, R z2  and R z3  are the same as above. In certain embodiments, a nucleoside 3′-phosphoramidite derivative is a chiral monomer which is used to produce stereocontrolled phosphorous atom-modified nucleotide and oligonucleotide. Examples of the nucleoside 3′-phosphoramidite derivatives are represented by the following formulae: 12a, 12b, 13a, 13b, 14a, 14b, 15a, 15b, 16a, 16b, 17a, 17b, 18a, 18b, 19a, 19b, 20a, 20b, 21a, 21b, 22a, 22b, 23a, 23b, 24a, 24b, 25a, 25b, 26a, 26b, 27a, 27b, 28a, 28b, 29a, 29b, 30a, 30b, 31a, 31b, 32a, 32b, 33a, 33b, 34a, 34b and 35a.
 
                                                                                                                                                                                                                                                                                     
DMTr represents a 4,4′-dimethoxytrityl group and TOM represents a triisopropylsiloxymethyl group.
 
     Examples using a nucleoside 3′-phosphoramidite derivative are disclosed in, e.g, JP 2005-89441 A. By repeating steps of condensation and de-protection, methods of the present invention facilitate lengthening the chain of oligonucleotide, as disclosed therein. 
     In some embodiments, an oligonucleotide is as shown in formula (Z-X): 
                         
wherein, in the formula (Z-X), X z  represents sulfide (═S), C 1-3  alkyl, C 1-3  alkoxy, C 1-3  alkylthio, C 6 -C 10  aryl, C 6 -C 10  aralkyl, or C 6 -C 10  aryloxialkyl. In some embodiments, X z  represents sulfide (═S). n z  is an integer that represents 1 to 150, 1 to 100, 1 to 50, or 1 to 30. In some embodiments, n z  is preferably 2 to 100, preferably 10 to 100, preferably 10 to 50, and more preferably 15 to 30.
 
     In some embodiments, the present invention provides methods for synthesis of a stereocontrolled phosphorus atom-modified oligonucleotide derivative. In some embodiments, the first step is a step of reacting a molecule comprising an achiral H-phosphonate moiety, the first activating reagent and a chiral reagent or a salt thereof to form a monomer. In some embodiments, the chiral reagent has chemical formula (Z-I) or (Z-I′) and the monomer may be represented by fomura (Z-Va), (Z-Vb), (Z-Va′), or (Z-Vb′). The monomer reacts with the second activating reagent and a nucleoside to form a condensed intermediate. Next step is a step of converting the condensed intermediate to the nucleic acid comprising a chiral X-phosphonate moiety. In some embodiments, the methods are as described in WO 2010/064146. In some embodiments, the steps are as described in route A and route B of WO 2010/064146. 
     In some embodiments, the present invention provides a method of synthesizing chirally controlled oligonucleotide as illustrated in Scheme Z-1 below. 
                         
Activation
 
     An achiral H-phosphonate moiety is treated with the first activating reagent to form the first intermediate. In one embodiment, the first activating reagent is added to the reaction mixture during the condensation step. Use of the first activating reagent is dependent on reaction conditions such as solvents that are used for the reaction. Examples of the first activating reagent are phosgene, trichloromethyl chloroformate, bis(trichloromethyl)carbonate (BTC), oxalyl chloride, Ph 3 PCl 2 , (PhO) 3 PCl 2 , N,N′-bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BopCl), 1,3-dimethyl-2-(3-nitro-1,2,4-triazol-1-yl)-2-pyrrolidin-1-yl-1,3,2-diazaphospholidinium hexafluorophosphate (MNTP), or 3-nitro-1,2,4-triazol-1-yl-tris(pyrrolidin-1-yl)phosphonium hexafluorophosphate (PyNTP). 
     The example of achiral H-phosphonate moiety is a compound shown in the above Scheme. DBU represents 1,8-diazabicyclo[5.4.0]undec-7-ene. H + DBU may be, for example, ammonium ion, alkylammonium ion, heteroaromatic iminium ion, or heterocyclic iminium ion, any of which is primary, secondary, tertiary or quaternary, or a monovalent metal ion. 
     Reacting with Chiral Reagent 
     After the first activation step, the activated achiral H-phosphonate moiety reacts with a chiral reagent, which is represented by formula (Z-I) or (Z-I′), to form a chiral intermediate of formula (Z-Va), (Z-Vb), (Z-Va′), or (Z-Vb′). 
     Stereospecific Condensation Step 
     A chiral intermediate of Formula Z-Va ((Z-Vb), (Z-Va′), or (Z-Vb′)) is treated with the second activating reagent and a nucleoside to form a condensed intermediate. The nucleoside may be on solid support. Examples of the second activating reagent are 4,5-dicyanoimidazole (DCI), 4,5-dichloroimidazole, 1-phenylimidazolium triflate (PhIMT), benzimidazolium triflate (BIT), benztriazole, 3-nitro-1,2,4-triazole (NT), tetrazole, 5-ethylthiotetrazole (ETT), 5-benzylthiotetrazole (BTT), 5-(4-nitrophenyl)tetrazole, N-cyanomethylpyrrolidinium triflate (CMPT), N-cyanomethylpiperidinium triflate, N-cyanomethyldimethylammonium triflate. A chiral intermediate of Formula Z-Va ((Z-Vb), (Z-Va′), or (Z-Vb′)) may be isolated as a monomer. Usually, the chiral intermediate of Z-Va ((Z-Vb), (Z-Va′), or (Z-Vb′)) is not isolated and undergoes a reaction in the same pot with a nucleoside or modified nucleoside to provide a chiral phosphite compound, a condensed intermediate. In other embodiments, when the method is performed via solid phase synthesis, the solid support comprising the compound is filtered away from side products, impurities, and/or reagents. 
     Capping Step 
     If the final nucleic acid is larger than a dimer, the unreacted —OH moiety is capped with a blocking group and the chiral auxiliary in the compound may also be capped with a blocking group to form a capped condensed intermediate. If the final nucleic acid is a dimer, then the capping step is not necessary. 
     Modifying Step 
     The compound is modified by reaction with an electrophile. The capped condensed intermediate may be executed modifying step. In some embodiments, the modifying step is performed using a sulfur electrophile, a selenium electrophile or a boronating agent. Examples of modifying steps are step of oxidation and sulfurization. 
     In some embodiments of the method, the sulfur electrophile is a compound having one of the following formulas:
 
S 8  (Formula Z-B), Z z1 —S—S—Z z2 , or Z z1 —S—V z —Z z2 ;
 
wherein Z z1  and Z z2  are independently alkyl, aminoalkyl, cycloalkyl, heterocyclic, cycloalkylalkyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, heteroaryloxy, acyl, amide, imide, or thiocarbonyl, or Z z1  and Z z2  are taken together to form a 3 to 8 membered alicyclic or heterocyclic ring, which may be substituted or unsubstituted; V z  is SO 2 , O, or NR f ; and R f  is hydrogen, alkyl, alkenyl, alkynyl, or aryl.
 
     In some embodiments of the method, the sulfur electrophile is a compound of following Formulae Z-A, Z-B, Z-C, Z-D, Z-E, or Z-F: 
     
       
         
         
             
             
         
       
     
     In some embodiments, the selenium electrophile is a compound having one of the following formulae:
 
Se (Formula Z-G), Z 3 —Se—Se—Z z4 , or Z z3 —Se—V—Z z4 ;
 
wherein Z z3  and Z z4  are independently alkyl, aminoalkyl, cycloalkyl, heterocyclic, cycloalkylalkyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, heteroaryloxy, acyl, amide, imide, or thiocarbonyl, or Z z3  and Z z4  are taken together to form a 3 to 8 membered alicyclic or heterocyclic ring, which may be substituted or unsubstituted; V z  is SO 2 , S, O, or NR f ; and R f  is hydrogen, alkyl, alkenyl, alkynyl, or aryl.
 
     In some embodiments, the selenium electrophile is a compound of Formula Z-G, Z-H, Z-I, Z-J, Z-K, or Z-L. 
     
       
         
         
             
             
         
       
     
     In some embodiments, the boronating agent is borane-N,N-diisopropylethylamine (BH 3  DIPEA), borane-pyridine (BH 3  Py), borane-2-chloropyridine (BH 3  CPy), borane-aniline (BH 3  An), borane-tetrahydrofiirane (BH 3  THF), or borane-dimethylsulfide (BH 3  Me 2 S). 
     In some embodiments of the method, the modifying step is an oxidation step. In some embodiments of the method, the modifying step is an oxidation step using similar conditions as described above in this application. In some embodiments, an oxidation step is as disclosed in, e.g., JP 2010-265304 A and WO2010/064146. 
     Chain Elongation Cycle and De-Protection Step 
     The capped condensed intermediate is deblocked to remove the blocking group at the 5′-end of the growing nucleic acid chain to provide a compound. The compound is optionally allowed to re-enter the chain elongation cycle to form a condensed intermediate, a capped condensed intermediate, a modified capped condensed intermediate, and a 5′-deprotected modified capped intermediate. Following at least one round of chain elongation cycle, the 5′-deprotected modified capped intermediate is further deblocked by removal of the chiral auxiliary ligand and other protecting groups for, e.g., nucleobase, modified nucleobase, sugar and modified sugar protecting groups, to provide a nucleic acid. In other embodiments, the nucleoside comprising a 5′-OH moiety is an intermediate from a previous chain elongation cycle as described herein. In yet other embodiments, the nucleoside comprising a 5′-OH moiety is an intermediate obtained from another known nucleic acid synthetic method. In embodiments where a solid support is used, the phosphorus-atom modified nucleic acid is then cleaved from the solid support. In certain embodiments, the nucleic acids is left attached on the solid support for purification purposes and then cleaved from the solid support following purification. 
     In yet other embodiments, the nucleoside comprising a 5′-OH moiety is an intermediate obtained from another known nucleic acid synthetic method. In yet other embodiments, the nucleoside comprising a 5′-OH moiety is an intermediate obtained from another known nucleic acid synthetic method as described in this application. In yet other embodiments, the nucleoside comprising a 5′-OH moiety is an intermediate obtained from another known nucleic acid synthetic method comprising one or more cycles illustrated in Scheme I. In yet other embodiments, the nucleoside comprising a 5′-OH moiety is an intermediate obtained from another known nucleic acid synthetic method comprising one or more cycles illustrated in Scheme I-b, I-c or I-d. 
     In some embodiments, the present invention provides oligonucleotide synthesis methods that use stable and commercially available materials as starting materials. In some embodiments, the present invention provides oligonucleotide synthesis methods to produce stereocontrolled phosphorus atom-modified oligonucleotide derivatives using an achiral starting material. 
     In some embodiments, the method of the present invention does not cause degradations under the de-protection steps. Further the method does not require special capping agents to produce phosphorus atom-modified oligonucleotide derivatives. 
     In some embodiments, the present invention provides methods for the synthesis of stereocontrolled phosphorus atom-modified oligonucleotide derivatives using a chiral monomer. In some embodiments, the first step is reacting a nucleoside 3′-phosphoramidite derivative which is represented by formula (Z-Va), (Z-Vb), (Z-Va′), or (Z-Vb′) with the second activating reagent and a nucleoside to form a condensed intermediate. The second step is converting the condensed intermediate to the nucleic acid comprising a chiral X-phosphonate moiety. An exemplary method is illustrated Scheme Z-2 below. 
     
       
         
         
             
             
         
       
     
     The detailed conditions of the Scheme Z-2 are similar to that of Scheme Z-1. The starting material of formula Z-Va (Z-Vb), especially of formula Z-Va′ (or Z-Vb′), is chemically stable. As shown in a working example, the method of the present invention does not cause degradations under the de-protection steps. Further the method does not require special capping agents to produce phosphorus atom-modified oligonucleotide derivatives. 
     In some embodiments, mechanism for the removal of auxiliaries is shown as illustrated in Scheme Z-3, below. 
                         
In Scheme Z-3, Nu stands for is a nucleophile. In some embodiments, the mechanism in Scheme Z-3 is thought to be different from the previous mechanism for the removal of auxiliaries.
 
     In some embodiments, the present invention provides a chiral reagent or a salt thereof, the chiral reagent having following chemical formula (Z-I): 
                         
wherein G z1  and G z2  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group, a group of formula (Z-II) or (Z-III), or both G z1  and G z2  taken together to form a group of formula (Z-IV),
 
                         
wherein G 21  to G 23  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group,
 
                         
wherein G 31  to C 33  are independently C 1-4  alkyl group, C 1-4  alkoxy group, C 6-14  aryl group, C 7-14  aralkyl group, C 1-4  alkyl C 6-14  aryl group, C 1-4  alkoxy C 6-14  aryl group, or C 6-14  aryl C 1-4  alkyl group,
 
                         
wherein G 41  to G 46  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group,
 
G z3  and G z4  are independently a hydrogen atom, C 1-3  alkyl group, C 6-14  aryl group, or both G z3  and G z4  taken together to form a heteroatom-containing ring that has 3 to 16 carbon atoms.
 
     In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula Z-1 has following chemical formula (Z-I′) 
                         
wherein each variable is independently as defined above and described herein. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein each of G z1  and G z2  is a group of formula (Z-II), wherein G 21  to G 23  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein each of G z1  and G z2  is a group of formula (Z-II), wherein each of G 21  to G 23  is a hydrogen atom. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  is a hydrogen atom, and G z2  is a group of formula (Z-II), wherein G 21  to G 23  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  is a hydrogen atom, and G z2  is a group of formula (Z-II), wherein each of G 21  and G 22  is a hydrogen atom and G 23  is a nitro group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  is a hydrogen atom, and G z2  is a group of formula (Z-III), wherein G 31  to G 33  are independently C 1-4  alkyl group, C 6-14  aryl group, C 7-14  aralkyl group, C 1-4  alkyl C 6-14  aryl group, C 1-4  alkoxy C 6-14  aryl group, or C 6-14  aryl C 1-4  alkyl group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  is a hydrogen atom, and G z2  is a group of formula (Z-III), wherein G 31  to G 33  are independently C 1-4  alkyl group, C 6  aryl group, C 7-10  aralkyl group, C 1-4  alkyl C 6  aryl group, C 1-4  alkoxy C 6  aryl group, or C 6  aryl C 1-4  alkyl group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  is a hydrogen atom, and G z2  is a group of formula (Z-III), wherein G 31  to G 33  are independently C 1-4  alkyl group, or C 6  aryl group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  is a hydrogen atom, and G z2  is a group of formula (Z-III), wherein G 31  and G 33  are C 6  aryl group and G 32  is C 1-2  alkyl group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  is a hydrogen atom, and G z2  is a group of formula (Z-III), wherein G 31  to G 33  are independently C 1-4  alkyl group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  is a hydrogen atom, and G z2  is a group of formula (Z-III), wherein G 31  and G 33  are C 6  aryl group and G 32  is C 1-4  alkyl group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  is a hydrogen atom, and G z2  is a group of formula (Z-IV), wherein G 41  to G 46  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  and G z2  are taken together to form a group of formula (Z-IV), wherein G 41  to G 46  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group. In some embodiments, the present invention provides a chiral reagent, or a salt thereof, of formula (Z-1′), wherein G z1  and G z2  are taken together to form a group of formula (Z-IV), wherein each of G 41  to G 46  is a hydrogen atom.
 
     In some embodiments, a chiral reagent or a salt thereof is selected from formulae 3a, 3b, 5a, Z-5b, 7a, 7b, 9a, 9b, 11a and 11b. 
     In some embodiments, the present invention provides a nucleoside 3′-phosphoramidite derivative which is represented by formula Z-Va or Z-Vb: 
                         
wherein G z1  and G z2  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group, a group of formula (Z-II) or (Z-III), or both G z1  and G z2  taken together to form a group of formula (Z-IV),
 
                         
wherein G 21  to G 23  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group,
 
                         
wherein G 31  to G 33  are independently C 1-4  alkyl group, C 1-4  alkoxy group, C 6-14  aryl group, C 7-14  aralkyl group, C 1-4  alkyl C 6-14  aryl group, C 1-4  alkoxy C 6-14  aryl group, or C 6-14  aryl C 1-4  alkyl group,
 
                         
wherein G 41  to G 46  are independently a hydrogen atom, a nitro group, a halogen atom, a cyano group or C 1-3  alkyl group;
 
G z3  and G z4  are independently a hydrogen atom, C 1-3  alkyl group, C 6-14  aryl group, or both G z3  and G z4  taken together to form a heteroatom-containing ring that has 3 to 16 carbon atoms;
 
G z5  is a protective group of a hydroxyl group;
 
R z2  is independently hydrogen, —OH, —SH, —NR d R d , —N 3 , halogen, alkyl, alkenyl, alkynyl, alkyl-Y 1 —, alkenyl-Y 1 —, alkynyl-Y 1 —, aryl-Y 1 —, heteroaryl-Y 1 —, —OR b , or —SR b , wherein R b  is a blocking moiety;
 
Y 1  is O, NR d , S, or Se;
 
R d  is independently hydrogen, alkyl, alkenyl, alkynyl, aryl, acyl, substituted silyl, carbamate, —P(O)(R e ) 2 , or —HP(O)(R e );
 
R e  is independently hydrogen, alkyl, aryl, alkenyl, alkynyl, alkyl-Y 2 —, alkenyl-Y 2 —, alkynyl-Y 2 —, aryl-Y 2 —, or heteroaryl-Y 2 —, or a cation which is Na + , Li + , or K + , or —O − ;
 
Y 2  is O, NR d , or S;
 
R z3  is a group represented by —CH 2 —, —(CH 2 ) 2 —, —CH 2 NH—, or —CH 2 N(CH 3 )—; and
 
Bs is a group selected from the groups represented by following formula (Z-VI) to (Z-XI) or derivatives thereof.
 
     
       
         
         
             
             
         
       
     
     In some embodiments, the present invention provides a nucleoside 3′-phosphoramidite derivative of formula Z-Va or Z-Vb, having the structure of (Z-Va′) or (Z-Vb′): 
                         
wherein each variable is independently as defined above and described herein.
 
     In some embodiments, the present invention provides a nucleoside 3′-phosphoramidite derivative selected from formulae 12a, 12b, 13a, 13b, 14a, 14b, 15a, 15b, 16a, 16b, 17a, 17b, 18a, 18b, 19a, 19b, 20a, 20b, 21a, 21b, 22a, 22b, 23a, 23b, 24a, 24b, 25a, 25b, 26a, 26b, 27a, 27b, 28a, 28b, 29a, 29b, 30a, 30b, 31a, 31b, 32a, 32b, 33a, 33b, 34a, 34b and 35a. In some embodiments, the present invention provides a nucleoside 3′-phosphoramidite derivative selected from formulae 12a, 12b, 13a, 13b, 14a, 14b, 15a, 15b, 16a, 16b, 17a, 17b, 18a, 18b, 19a, 19b, 20a, 20b, 21a, 21b, 22a, 22b, 23a, 23b, 24a, 24b, 25a, 25b, 26a, or 26b. 
     In some embodiments, the present invention provides a method for synthesis of stereocontrolled phosphorus atom-modified oligonucleotide derivatives comprising steps of: 
     reacting a molecule comprising an achiral H-phosphonate moiety, a chiral reagent or a salt thereof to form a monomer of a nucleoside 3′-phosphoramidite derivative; 
     reacting the monomer and a nucleoside to form a condensed intermediate; and 
     converting the condensed intermediate to the nucleic acid comprising a chiral X-phosphonate moiety; 
     wherein the chiral reagent has following chemical formula (Z-I). 
     In some embodiments, the present invention provides a method for synthesis of stereocontrolled phosphorus atom-modified oligonucleotide derivatives comprising steps of: 
     reacting a molecule comprising an achiral H-phosphonate moiety, a chiral reagent or a salt thereof to form a monomer of a nucleoside 3′-phosphoramidite derivative; 
     reacting the monomer and a nucleoside to form a condensed intermediate; and 
     converting the condensed intermediate to the nucleic acid comprising a chiral X-phosphonate moiety; 
     wherein the chiral reagent has following chemical formula (Z-I′). 
     In some embodiments, the present invention provides a method for synthesis of stereocontrolled phosphorus atom-modified oligonucleotide derivatives comprising steps of: 
     reacting a molecule comprising an achiral H-phosphonate moiety, a chiral reagent or a salt thereof to form a monomer of a nucleoside 3′-phosphoramidite derivative; 
     reacting the monomer and a nucleoside to form a condensed intermediate; and 
     converting the condensed intermediate to the nucleic acid comprising a chiral X-phosphonate moiety; 
     wherein the chiral reagent is selected from formulae 3a, 3b, 5a, Z-5b, 7a, 7b, 9a, 9b, 11a and 11b. 
     In some embodiments, the present invention provides a method for synthesis of stereocontrolled phosphorus atom-modified oligonucleotide derivatives comprising steps of: 
     reacting a nucleoside 3′-phosphoramidite derivative which is represented by formula (Z-Va) or (Z-Vb), with an activating reagent and a nucleoside to form a condensed intermediate; and converting the condensed intermediate to the nucleic acid comprising a chiral X-phosphonate moiety. 
     In some embodiments, the present invention provides a method for synthesis of stereocontrolled phosphorus atom-modified oligonucleotide derivatives comprising steps of: 
     reacting a nucleoside 3′-phosphoramidite derivative represented by formula (Z-Va) or (Z-Vb), with an activating reagent and a nucleoside to form a condensed intermediate; and converting the condensed intermediate to the nucleic acid comprising a chiral X-phosphonate moiety; and wherein the nucleoside 3′-phosphoramidite derivative represented by formula (Z-Va) or (Z-Vb) is selected from formulae 12a, 12b, 13a, 13b, 14a, 14b, 15a, 15b, 16a, 16b, 17a, 17b, 18a, 18b, 19a, 19b, 20a, 20b, 21a, 21b, 22a, 22b, 23a, 23b, 24a, 24b, 25a, 25b, 26a, 26b, 27a, 27b, 28a, 28b, 29a, 29b, 30a, 30b, 31a, 31b, 32a, 32b, 33a, 33b, 34a, 34b and 35a. In some embodiments, the present invention provides a method for synthesis of stereocontrolled phosphorus atom-modified oligonucleotide derivatives comprising steps of:
 
reacting a nucleoside 3′-phosphoramidite derivative represented by formula (Z-Va) or (Z-Vb), with an activating reagent and a nucleoside to form a condensed intermediate; and converting the condensed intermediate to the nucleic acid comprising a chiral X-phosphonate moiety; and wherein the nucleoside 3′-phosphoramidite derivative represented by formula (Z-Va) or (Z-Vb) is selected from formulae 12a, 12b, 13a, 13b, 14a, 14b, 15a, 15b, 16a, 16b, 17a, 17b, 18a, 18b, 19a, 19b, 20a, 20b, 21a, 21b, 22a, 22b, 23a, 23b, 24a, 24b, 25a, 25b, 26a, and 26b.
 
Preparation and Use of Certain Chiral Auxiliaries of Formula Z-I
 
     Abbreviation 
     
         
         ac: acetyl 
         bz: benzoyl 
         CSO: (1S)-(+)-(10-camphorsulfonyl)oxaziridine 
         DBU: 1,8-diazabicyclo[5.4.0]undec-7-ene 
         DCA: dichloroacetic acid 
         DCM: dichloromethane, CH 2 Cl 2    
         Tr: trityl, triphenylmethyl 
         MeIm: N-methylimidazole 
         NIS: N-iodosuccinimide 
         pac: phenoxyacetyl 
         Ph: phenyl 
         PhIMT: N-phenylimidazolium triflate 
         POS: 3-phenyl-1,2,4-dithiazoline-5-one 
         TBS: tert-butyldimethylsilyl 
         TBDPS: tert-butyldiphenylsilyl 
         TOM: triisopropylsiloxymethyl 
         TFA: trifluoroacetic acid
 
General Procedure for the Synthesis of Chirally Controlled Oligonucleotides-1.
 
       
    
     The automated solid-phase synthesis of chirally controlled oligonucleotides was performed according to the cycles shown in Table Z-1. 
                     TABLE Z-1                  Synthesis procedure.                                 step   operation   reagents and solvent   volume   waiting time                                             1   detritylation   3% DCA/DCM   1.6 mL   20   s       2   coupling   0.1M monomer/MeCN + 1M PhIMT   0.5 mL   5   min       3   capping   Ac 2 O/THF-pyridine + 16% MeIm/THF   0.5 mL   30   s       4   oxidation/sulfurization   0.5M CSO/MeCN or 0.1M POS/MeCN   0.5 mL   90   s                    
General Procedure for the Synthesis of Chirally Controlled Oligonucleotides-2.
 
     The automated solid-phase synthesis of chirally controlled oligonucleotides was performed according to the cycles shown in Table Z-2. 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE Z-2 
               
               
                   
               
               
                 step 
                 operation 
                 reagents and solvent 
                 volume 
                 waiting time 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 1 
                 detritylation 
                 3% DCA/DCM 
                 1.6 mL 
                 20 
                 s 
               
               
                 2 
                 coupling 
                 pre-activated monomer* + 1M PhIMT 
                 0.5 mL 
                 5 
                 min 
               
               
                 3 
                 capping 
                 Ac 2 O/THF-pyridine + 16% MeIm/THF 
                 0.5 mL 
                 30 
                 s 
               
               
                 4 
                 oxidation/sulfurization 
                 0.5M CSO/MeCN or 0.1M POS/MeCN 
                 0.5 mL 
                 90 
                 s 
               
               
                   
               
               
                 *preparation of pre-activated monomer in Step 2 of TableZ- 2: 
               
            
           
         
       
     
     Nucleoside-3′-H-phosphonate monoester is dried by repeated coevaporations with dry toluene and then dissolved in dry MeCN. Ph 3 PCl 2  is added to the solution, and the mixture is stirred for 5 min. To the mixture, a solution of chiral reagent, which is repeated coevaportions with dry toluene and dissolved in dry MeCN, is added dropwise via syringe, and the mixture is stirred for 5 min under argon. 
     After the synthesis, the resin was treated with a 25% NH 3  aqueous solution (1 mL) for 12 h at 55° C. The mixture was cooled to room temperature and the resin was removed by membrane filtration. The filtrate was concentrated to dryness under reduced pressure. The residue was dissolved in H 2 O (3 mL) and analyzed by RP-UPLC-MS with a linear gradient of acetonitrile (0-50%/30 min) in 0.1 M triethylammonium acetate buffer (pH 7.0) at 50° C. at a rate of 0.3 mL/min. 
     Example Z-1 
     (S)-1-Tritylpyrrolidin-2-carbaldehyde (1a) 
     
       
         
         
             
             
         
       
     
     Compound 1a was synthesized from L-proline according to the procedure described in the literature (Guga, P.  Curr. Top. Med. Chem.  2007, 7, 695-713). 
     (R)-1-Tritylpyrrolidin-2-carbaldehyde (1b) 
     
       
         
         
             
             
         
       
     
     Compound 1b was synthesized from D-proline in a similar manner to compound 1a. 
     (S)-2-(Methyldiphenylsilyl)-1-((S)-1-tritylpyrrolidin-2-yl)ethanol (2a) 
     
       
         
         
             
             
         
       
     
     To a solution of methyldiphenylsilylmethyl magnesium chloride in THF prepared from chloromethyldiphenylmethylsilane (4.02 g, 16.3 mmol) and magnesium (402 mg, 16.3 mmol) in THF (14 mL) was added 1a (2.79 g, 8.14 mmol) in THF (30 mL) solution with ice cooling. After stirring for 1.5 h with ice cooling, the mixture warmed to room temperature and continued stirring for 30 min. Saturated aqueous NH 4 Cl (100 mL) was added to the reaction mixture at 0° C., and extraction was performed with diethylether (100 mL) for three times. The combined extract was dried over Na 2 SO 4 , filtered and concentrated under reduced pressure. The residue was chromatographed on silica gel afforded 2a as a colorless foam (3.91 g, 87%).  1 H NMR (300 MHz, CDCl 3 ) δ 7.48-7.08 (25H, m), 4.33-4.23 (1H, m), 3.16-2.89 (3H, m), 2.84 (1H, brs), 1.70-1.54 (1H, m), 1.35 (1H, dd, J=14.7, 6.3 Hz), 1.10 (1H, dd, J=14.7, 8.1 Hz), 1.18-1.05 (1H, m), 1.04-0.90 (1H, m), 0.34 (3H, s), −0.17-−0.36 (1H, m). 
     (S)-2-(Methyldiphenylsilyl)-1-((S)-1-pyrrolidin-2-yl)ethanol (3a) 
     
       
         
         
             
             
         
       
     
     2a (3.91 g, 7.06 mmol) was dissolved in 3% DCA in DCM (70 mL), and stirred for 10 min at room temperature. To the mixture, 1M NaOH (200 mL) was added, and extraction was performed with DCM (100 mL) for three times. The combined extract was dried over Na 2 SO 4 , filtered and concentrated under reduced pressure. The residue was chromatographed on silica gel afforded 3a as a light yellow oil (1.99 g, 90%).  1 H NMR (300 MHz, CDCl 3 ) δ 7.57-7.52 (5H, m), 7.38-7.33 (5H, m), 3.77 (1H, ddd, J=8.9, 5.4, 3.5 Hz), 3.01 (1H, dt, J=7.4, 3.6 Hz), 2.97-2.79 (2H, m), 2.27 (2H, brs), 1.76-1.53 (4H, m), 1.38 (1H, dd, J=15.0, 9.0 Hz), 1.24 (1H, dd, J=15.0, 5.4 Hz), 0.65 (3H, s);  13 C NMR (100.4 MHz, CDCl 3 ) δ 137.4, 137.1, 134.6, 134.5, 129.1, 127.8, 69.5, 64.1, 47.0, 25.8, 24.0, 19.6, −3.4. MALDI TOF-MS m/z Calcd for C 19 H 26 NOSi [M+H] +  312.18, found 312.06. 
     Example Z-2 
     (R)-2-(Methyldiphenylsilyl)-1-((R)-1-tritylpyrrolidin-2-yl)ethanol (2b) 
     
       
         
         
             
             
         
       
     
     Compound 2b was obtained by using 1b instead of 1a in a similar manner to compound 2a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.48-7.12 (25H, m), 4.33-4.24 (1H, m), 3.16-2.89 (3H, m), 2.86 (1H, brs), 1.69-1.52 (1H, m), 1.35 (1H, dd, J=14.4, 6.0 Hz), 1.10 (1H, dd, J=14.4, 8.4 Hz), 1.18-1.05 (1H, m), 1.03-0.89 (1H, m), 0.33 (3H, s), −0.19-−0.39 (1H, m);  13 C NMR (75.5 MHz, CDCl 3 ) δ 144.5, 137.5, 136.8, 134.6, 134.3, 129.8, 129.0, 127.8, 127.7, 127.4, 126.1, 77.9, 71.7, 65.1, 53.5, 25.0, 24.8, 19.6, −4.0. MALDI TOF-MS m/z Calcd for C 38 H 40 NOSi [M+H] +  554.29, found 554.09. 
     (R)-2-(Methyldiphenylsilyl)-1-((R)-1-pyrrolidin-2-yl)ethanol (3b) 
     
       
         
         
             
             
         
       
     
     Compound 3b was obtained by using 2b instead of 2a in a similar manner to compound 3a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 7.58-7.52 (5H, m), 7.38-7.33 (5H, m), 3.78 (1H, ddd, J=9.0, 5.1, 3.6 Hz), 3.00 (1H, dt, J=7.4, 3.3 Hz), 2.97-2.78 (2H, m), 2.19 (2H, brs), 1.76-1.53 (4H, m), 1.38 (1H, dd, J=14.6, 9.0 Hz), 1.24 (1H, dd, J=14.6, 5.1 Hz), 0.66 (3H, s);  13 C NMR (75.5 MHz, CDCl 3 ) δ 137.5, 137.1, 134.5, 134.4, 129.0, 127.7, 69.2, 64.2, 46.9, 25.8, 24.0, 19.7, −3.4. MALDI TOF-MS m/z Calcd for C 19 H 26 NOSi [M+H] +  312.18, found 312.09. 
     Example Z-3 
     (S)-2-(Trimethylsilyl)-1-((S)-1-tritylpyrrolidin-2-yl)ethanol (4a) 
     
       
         
         
             
             
         
       
     
     Compound 4a was obtained by using “chloromethyltrimethylsilane” instead of “chloromethyldiphenylmethylsilane” in a similar manner to compound 2a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.58-7.51 (5H, m), 7.31-7.14 (10H, m), 4.13 (1H, dt, J=7.5, 3.0 Hz), 3.39-3.31 (1H, m), 3.20-2.99 (2H, m), 2.84 (1H, s), 1.74-1.57 (1H, m), 1.29-1.10 (2H, m), 0.74 (1H, dd, J=14.4, 7.2 Hz), 0.46 (1H, dd, J=14.4, 7.2 Hz), −0.15 (9H, s). MALDI TOF-MS m/z Calcd for C 28 H 36 NOSi [M+H] +  430.26, found 430.09. 
     (S)-2-(Trimethylsilyl)-1-((S)-1-pyrrolidin-2-yl)ethanol (5a) 
     
       
         
         
             
             
         
       
     
     Compound 5a was obtained by using 4a instead of 2a in a similar manner to compound 3a.  1 H NMR (300 MHz, CDCl 3 ) δ 3.76 (1H, ddd, J=8.8, 5.7, 3.3 Hz), 3.08 (1H, dt, J=7.8, 3.3 Hz), 3.02-2.87 (2H, m), 2.48 (2H, brs), 1.81-1.58 (4H, m), 0.83 (1H, dd, J=14.7, 8.7 Hz), 0.68 (1H, dd, J=14.7, 6.0 Hz), 0.05 (9H, s);  13 C NMR (75.5 MHz, CDCl 3 ) δ 69.6, 64.3, 46.9, 25.8, 23.9, 22.0, −0.8. MALDI TOF-MS m/z Calcd for C 9 H 22 NOSi [M+H] +  188.15, found 188.00. 
     Example Z-5 
     (R)-2,2-Diphenyl-1-((S)-1-tritylpyrrolidin-2-yl)ethanol (6a) 
     
       
         
         
             
             
         
       
     
     To a solution of diphenylmethane (6.7 mL, 40 mmol) in anhydrous THF (36 mL), n-BuLi (1.67M solution of Hexane, 24 mL, 40 mmol) was added dropwise at room temperature and stirred for 1 h. To the mixture, 1a (3.41 g, 10 mmol), which was dried by repeated coevaporations with toluene, in anhydrous THF (40 mL) was slowly added at 0° C., and continued stirring for 45 min. A saturated NH 4 Cl aqueous solution (100 mL) and Et 2 O (100 mL) were then added, and the organic layer was separated and the aqueous layer was extracted with Et 2 O (2×100 mL). The organic layer were combined, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure. The residue was purified by chromatography on silica gel to afford 6a (1.41 g, 28%) as white foam. 
     (R)-2,2-Diphenyl-1-((S)-pyrrolidin-2-yl)ethanol (7a) 
     
       
         
         
             
             
         
       
     
     6a (650 mg, 1.27 mmol) was dissolved in 3% DCA in DCM (13 mL), and stirred for 10 min at room temperature. To the mixture, 1M NaOH (40 mL) was added, and extraction was performed with DCM (30 mL) for three times. The combined extract was dried over Na 2 SO 4 , filtered and concentrated under reduced pressure. The residue was chromatographed on silica gel afforded 7a as a light yellow oil (316 mg, 93%).  1 H NMR (300 MHz, CDCl 3 ) δ 7.44-7.38 (2H, m), 7.33-7.14 (8H, m), 4.46 (1H, dd, J=9.9, 3.3 Hz), 3.91 (1H, d, J=9.9 Hz), 3.02-2.88 (2H, m), 2.81-2.69 (1H, m), 2.52 (2H, brs), 1.88-1.56 (4H, m);  13 C NMR (75.5 MHz, CDCl 3 ) δ 142.3, 142.0, 128.6, 128.5, 128.4, 128.2, 126.5, 126.4, 73.5, 60.1, 55.8, 46.6, 25.8, 23.4. MALDI TOF-MS m/z Calcd for C 18 H 22 NO [M+H] +  268.17, found 268.06. 
     Example Z-6 
     (S)-2,2-Diphenyl-1-((R)-1-tritylpyrrolidin-2-yl)ethanol (6b) 
     
       
         
         
             
             
         
       
     
     Compound 6b was obtained by using 1b instead of 1a in a similar manner to compound 6a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.44-7.37 (6H, m), 7.30-7.01 (17H, m), 6.66-6.61 (2H, m), 4.80 (1H, d, J=10.8 Hz), 3.63 (1H, d, J=10.8 Hz), 3.36-3.28 (1H, m), 3.22-3.09 (1H, m), 3.01-2.89 (1H, m), 2.66 (1H, s), 1.90-1.75 (1H, m), 1.29-1.04 (2H, m), 0.00-0.19 (1H, m);  13 C NMR (75.5 MHz, CDCl 3 ) δ 144.2, 142.9, 141.6, 130.0, 128.5, 128.4, 127.9, 127.8, 127.4, 126.4, 126.2, 77.9, 75.9, 61.9, 55.4, 53.4, 24.7, 24.5. MALDI TOF-MS m/z Calcd for C 37 H 36 NO [M+H] +  510.28, found 510.11. 
     (S)-2,2-Diphenyl-1-((R)-pyrrolidin-2-yl)ethanol (7b) 
     
       
         
         
             
             
         
       
     
     Compound 7b was obtained by using 6b instead of 6a in a similar manner to compound 7a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.45-7.14 (10H, m), 4.45 (1H, dd, J=9.9, 3.3 Hz), 3.91 (1H, d, J=9.9 Hz), 3.00-2.89 (2H, m), 2.82-2.71 (1H, m), 2.40 (2H, brs), 1.87-1.55 (4H, m);  13 C NMR (75.5 MHz, CDCl 3 ) δ 142.3, 142.0, 128.5, 128.3, 128.1, 126.3, 126.2, 73.4, 60.1, 55.9, 46.5, 25.8, 23.5. MALDI TOF-MS m/z Calcd for C 18 H 22 NO [M+H] +  268.17, found 268.03. 
     Example Z-7 
     (R)-2-(4-Nitrophenyl)-1-((S)-1-tritylpyrrolidin-2-yl)ethanol (8a) 
     
       
         
         
             
             
         
       
     
     Compound 8a was obtained by using “4-nitrobenzylchloride” instead of “diphenylmethane” in a similar manner to compound 6a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.09-8.03 (2H, m), 7.49-7.43 (6H, m), 7.28-7.09 (11H, m), 4.23 (1H, ddd, J=8.3, 5.6, 3.0 Hz), 3.43-3.33 (1H, m), 3.23-3.11 (1H, m), 3.07-2.96 (1H, m), 2.83 (1H, brs), 2.74 (1H, dd, J=13.8, 8.4 Hz), 2.49 (1H, dd, J=13.8, 5.1 Hz), 1.83-1.67 (1H, m), 1.41-1.17 (2H, m), 0.27-0.08 (1H, m);  13 C NMR (75.5 MHz, CDCl 3 ) δ 147.3, 146.3, 144.3, 129.8, 129.6, 127.5, 126.3, 123.4, 77.9, 74.8, 63.5, 53.2, 39.5, 25.0, 24.9. MALDI TOF-MS m/z Calcd for C 31 H 31 N 2 O 3  [M+H] +  479.23, found 479.08. 
     (R)-2-(4-Nitrophenyl)-1-((S)-pyrrolidin-2-yl)ethanol (9a) 
     
       
         
         
             
             
         
       
     
     Compound 9a was obtained by using 8a instead of 6a in a similar manner to compound 7a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.15 (2H, d, J=8.7 Hz), 7.42 (2H, d, J=8.7 Hz), 3.86-3.79 (1H, m), 3.16-3.07 (1H, m), 2.99-2.68 (6H, m), 1.84-1.68 (4H, m);  13 C NMR (75.5 MHz, CDCl 3 ) δ 147.4, 146.2, 129.9, 123.2, 72.4, 62.0, 46.6, 40.4, 25.7, 24.4. MALDI TOF-MS m/z Calcd for C 12 H 17 N 2 O 3  [M+H] +  237.12, found 237.01. 
     Example Z-8 
     (S)-2-(4-Nitrophenyl)-1-((R)-1-tritylpyrrolidin-2-yl)ethanol (8b) 
     
       
         
         
             
             
         
       
     
     Compound 8b was obtained by using 1b instead of 1a in a similar manner to compound 8a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.09-8.04 (2H, m), 7.49-7.43 (6H, m), 7.28-7.09 (11H, m), 4.22 (1H, ddd, J=8.4, 5.6, 3.0 Hz), 3.43-3.33 (1H, m), 3.24-3.10 (1H, m), 3.08-2.94 (1H, m), 2.81 (1H, brs), 2.75 (1H, dd, J=14.0, 8.1 Hz), 2.49 (1H, dd, J=14.0, 5.1 Hz), 1.81-1.67 (1H, m), 1.40-1.16 (2H, m), 0.26-0.09 (1H, m);  13 C NMR (75.5 MHz, CDCl 3 ) δ 147.3, 144.3, 129.8, 129.6, 129.4, 126.3, 123.5, 77.9, 74.8, 63.5, 53.2, 39.5, 25.0, 24.9. MALDI TOF-MS m/z Calcd for C 31 H 31 N 2 O 3  [M+H] +  479.23, found 479.08. 
     (S)-2-(4-Nitrophenyl)-1-((R)-pyrrolidin-2-yl)ethanol (9b) 
     
       
         
         
             
             
         
       
     
     Compound 9b was obtained by using 8b instead of 8a in a similar manner to compound 9a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.19-8.13 (2H, m), 7.45-7.39 (2H, m), 3.83 (1H, ddd, J=7.7, 5.4, 3.9 Hz), 3.14 (1H, dt, J=7.7, 3.9 Hz), 3.01-2.87 (2H, m), 2.83 (1H, d, J=3.3 Hz), 2.81 (1H, s), 2.62 (2H, brs), 1.79-1.72 (4H, m);  13 C NMR (75.5 MHz, CDCl 3 ) δ 147.3, 146.5, 130.0, 123.5, 72.7, 61.7, 46.7, 40.1, 25.8, 24.2. MALDI TOF-MS m/z Calcd for C 12 H 17 N 2 O 3  [M+H] +  237.12, found 237.02. 
     Example Z-9 
     (R)-(9H-Fluoren-9-yl)((S)-1-tritylpyrrolidin-2-yl)methanol (10a) 
     
       
         
         
             
             
         
       
     
     Compound 10a was obtained by using “fluorene” instead of “diphenylmethane” in a similar manner to compound 6a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.70 (1H, d, J=7.5 Hz), 7.66 (1H, d, J=7.8 Hz), 7.55 (2H, d, J=7.5 Hz), 7.44-7.09 (18H, m), 6.87-6.62 (1H, m), 4.55-4.48 (1H, m), 4.06 (1H, d, J=7.5 Hz), 3.43-3.34 (1H, m), 3.18-3.06 (1H, m), 2.98-2.88 (1H, m), 2.85 (1H, brs), 1.42-1.24 (1H, m), 1.18-1.04 (1H, m), 0.53-0.39 (1H, m), −0.02-−0.20 (1H, m); MALDI TOF-MS m/z Calcd for C 37 H 34 NO [M+H] +  508.26, found 508.12. 
     (R)-(9H-Fluororen-9-yl)((S)-pyrrolidin-2-yl)methanol (11a) 
     
       
         
         
             
             
         
       
     
     Compound 11a was obtained by using 10a instead of 6a in a similar manner to compound 7a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.76 (2H, d, J=7.5 Hz), 7.68 (2H, t, J=8.0 Hz), 7.43-7.35 (2H, m), 7.34-7.25 (2H, m), 4.28 (1H, d, J=6.3 Hz), 4.03 (1H, dd, J=6.5, 4.2 Hz), 3.19-3.11 (1H, m), 2.97-2.88 (1H, m), 2.86-2.76 (1H, m), 2.02 (2H, brs), 1.77-1.53 (3H, m), 1.38-1.23 (1H, m); MALDI TOF-MS m/z Calcd for C 18 H 20 NO [M+H] +  266.15, found 266.04. 
     (S)-2-Tosyl-1-((S)-1-tritylpyrrolidin-2-yl)ethanol (12a′) 
     
       
         
         
             
             
         
       
     
     Compound 12a′ was obtained by using “chloromethyl p-tolyl sulfone” instead of “chloromethyldiphenylmethylsilane” in a similar manner to compound 2a. 
       1 H NMR (600 MHz, CDCl 3 ) δ 7.66 (2H, d, J=8.4 Hz), 7.48-7.44 (6H, m), 7.35 (2H, d, J=7.2 Hz), 7.21-7.13 (9H, m), 4.39-4.36 (1H, m), 3.33 (1H, s), 3.24-3.20 (1H, m), 3.19-3.10 (2H, m), 2.98-2.92 (2H, m), 2.49 (3H, s), 1.55-1.49 (1H, m), 1.33-1.26 (1H, m), 1.12-1.04 (1H, m), 0.22-0.14 (1H, m);  13 C NMR (150.9 MHz, CDCl 3 ) δ 144.6, 144.5, 136.3, 129.9, 129.5, 128.1, 127.5, 126.2, 78.0, 69.1, 63.9, 60.2, 52.6, 25.5, 24.7, 21.7. 
     (S)-2-Tosyl-1-((S)-1-tritylpyrrolidin-2-yl)ethanol (13a′) 
     
       
         
         
             
             
         
       
     
     Compound 13a′ was obtained by using 12a′ instead of 2a in a similar manner to compound 3a. 
       1 H NMR (600 MHz, CDCl 3 ) δ 7.82 (2H, d, J=8.4 Hz), 7.37 (2H, d, J=8.4 Hz), 4.01 (1H, ddd, J=12.0, 5.1, 3.0 Hz), 3.32 (1H, dd, J=14.4, 3.0 Hz), 3.25 (1H, dd, J=14.4, 9.0 Hz), 3.16 (1H, dt, J=7.8, 5.1 Hz), 2.90-2.82 (2H, m), 2.46 (3H, s), 2.04 (2H, brs), 1.78-1.63 (3H, m), 1.62-1.55 (1H, m);  13 C NMR (150.9 MHz, CDCl 3 ) δ 144.5, 136.7, 129.7, 127.7, 67.4, 61.8, 60.1, 46.7, 25.7, 21.4. MALDI TOF-MS m/z Calcd for C 13 H 20 NO 3 S [M+H] +  270.12, found 270.04. 
     (R)-2-Tosyl-1-((R)-1-tritylpyrrolidin-2-yl)ethanol (12b′) 
     
       
         
         
             
             
         
       
     
     Compound 12b′ was obtained by using 1b instead of 1a in a similar manner to compound 12a′. 
       1 H NMR (600 MHz, CDCl 3 ) δ 7.66 (2H, d, J=8.4 Hz), 7.47-7.44 (6H, m), 7.35 (2H, d, J=7.8 Hz), 7.21-7.13 (9H, m), 4.37 (1H, dt, J=8.6, 2.4 Hz), 3.33 (1H, s), 3.23-3.20 (1H, m), 3.19-3.12 (2H, m), 2.98-2.92 (2H, m), 2.49 (3H, s), 1.56-1.49 (1H, m), 1.32-1.26 (1H, m), 1.11-1.03 (1H, m), 0.23-0.15 (1H, m);  13 C NMR (150.9 MHz, CDCl 3 ) δ 144.6, 144.5, 136.3, 129.9, 129.6, 128.1, 127.6, 126.2, 78.0, 69.1, 63.9, 60.2, 52.6, 25.5, 24.7, 21.7. 
     (R)-2-Tosyl-1-(R)-1-tritylpyrrolidin-2-yl)ethanol (13b′) 
     
       
         
         
             
             
         
       
     
     Compound 13b′ was obtained by using 12b′ instead of 12a′ in a similar manner to compound 13a′. 
       1 H NMR (600 MHz, CDCl 3 ) δ 7.82 (2H, d, J=8.4 Hz), 7.37 (2H, d, J=8.4 Hz), 4.01 (1H, ddd, J=9.0, 5.1, 3.0 Hz), 3.32 (1H, dd, J=14.4, 3.0 Hz), 3.25 (1H, dd, J=14.4, 9.0 Hz), 3.17 (1H, dt, J=7.2, 5.1 Hz), 2.89-2.83 (2H, m), 2.46 (3H, s), 2.04 (2H, brs), 1.79-1.64 (3H, m), 1.62-1.55 (1H, m);  13 C NMR (150.9 MHz, CDCl 3 ) δ 144.8, 136.6, 129.8, 127.9, 67.7, 61.8, 60.1, 46.8, 25.9, 25.8, 21.6. MALDI TOF-MS m/z Calcd for C 13 H 20 NO 3 S [M+H] +  270.12, found 270.05. 
     Example Z-10 
     Oxazaphospholidine Monomer 12a 
     
       
         
         
             
             
         
       
     
     3a (560 mg, 1.80 mmol) were dried by repeated coevaporations with dry toluene and dissolved in dry diethylether (0.90 mL) under argon. N-Methylmorpholine (400 micro L, 3.60 mmol) was added to the solution, and the resultant solution was added dropwise to a solution of PCl 3  (160 micro L, 1.80 mmol) in dry diethylether (0.90 mL) at 0° C. under argon with stirring. The mixture was then allowed to warm to room temperature and stirred for 30 min. The resultant N-methylmorpholine hydrochloride was removed by filtration under nitrogen, and the filtrate was concentrated to dryness under reduced pressure to afford crude 2-chloro-1,3,2-oxazaphospholidine derivative. The crude materials were dissolved in freshly distilled THF (3.6 mL) to make 0.5 M solutions, which were used to synthesize the nucleoside 3′-O-oxazaphospholidines without further purification. 
     5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine (636 mg, 0.84 mmol) was dried by repeated coevaporations with dry toluene, and dissolved in freshly distilled THF (2.5 mL) under argon. Et 3 N (0.58 mL, 4.2 mmol) was added, and the mixture was cooled to −78° C. A 0.5 M solution of the corresponding crude 2-chloro-1,3,2-oxazaphospholidine derivative in freshly distilled THF (3.6 mL, 1.80 mmol) was added dropwise via a syringe, and the mixture was stirred for 15 min at room temperature. A saturated NaHCO 3  aqueous solution (70 mL) and CHCl 3  (70 mL) were then added, and the organic layer was separated and washed with saturated NaHCO 3  aqueous solutions (2×70 mL). The combined aqueous layers were back-extracted with CHCl 3  (70 mL). The organic layers were combined, dried over Na 2 SO 4 , filtered and concentrated under reduced pressure. The residue was purified by chromatography on silica gel to afford 12a (829 mg, 90%) as a white foam.  1 H NMR (300 MHz, CDCl 3 ) δ 8.77 (1H, brs), 7.99 (1H, s), 7.54-6.98 (24H, m), 6.81-6.73 (4H, m), 6.35 (1H, dd, J=8.0, 6.3 Hz), 4.89-4.73 (411, m), 4.68 (2H, brs), 4.05-3.98 (1H, m), 3.75 (6H, s), 3.62-3.46 (1H, m), 3.41-3.20 (3H, m), 3.18-3.04 (1H, m), 3.08 (2H, t, J=6.6 Hz), 2.58-2.36 (2H, m), 1.94-1.59 (2H, m), 1.56 (1H, dd, J=15.0, 8.7 Hz), 1.43 (1H, dd, J=15.0, 5.7 Hz), 1.33-1.16 (2H, m), 0.62 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 153.5 (1P, s). 
     Example Z-11 
     Oxazaphospholidine Monomer 12b 
     
       
         
         
             
             
         
       
     
     Compound 12b was obtained by using 3b instead of 3a in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.80 (1H, brs), 7.96 (1H, s), 7.54-6.96 (24H, m), 6.79-6.71 (4H, m), 6.19 (1H, t, J=6.6 Hz), 4.90-4.73 (4H, m), 4.66 (2H, brs), 4.16-4.08 (1H, m), 3.76 (6H, s), 3.60-3.36 (2H, m), 3.29 (1H, d, J=3.9 Hz), 3.27-3.12 (2H, m), 3.09 (2H, t, J=6.6 Hz), 2.59-2.46 (1H, m), 2.07-1.97 (1H, m), 1.94-1.41 (5H, m), 1.36-1.18 (1H, m), 0.65 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 157.1 (1P, s). 
     Example Z-12 
     Oxazaphospholidine Monomer 13a 
     
       
         
         
             
             
         
       
     
     Compound 13a was obtained by using “5′-O-(DMTr)thymidine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.58-7.23 (21H, m), 6.86-6.79 (4H, m), 6.35 (1H, dd, J=8.1, 5.7 Hz), 4.79-4.67 (2H, m), 3.83-3.78 (1H, m), 3.78 (6H, s), 3.59-3.43 (1H, m), 3.34 (1H, dd, J=10.5, 2.4 Hz), 3.35-3.24 (1H, m), 3.20 (1H, dd, J=10.5, 2.4 Hz), 3.16-3.02 (1H, m), 2.36-2.26 (1H, m), 2.15-2.02 (1H, m), 1.92-1.77 (1H, m), 1.74-1.59 (1H, m), 1.52 (1H, dd, J=14.7, 9.0 Hz), 1.40 (3H, s), 1.45-1.15 (3H, m), 0.60 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 153.7 (1P, s). 
     Example Z-13 
     Oxazaphospholidine Monomer 13b 
     
       
         
         
             
             
         
       
     
     Compound 13b was obtained by using 3b instead of 3a in a similar manner to compound 13a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.46 (1H, brs), 7.59-7.20 (20H, m), 6.86-6.79 (4H, m), 6.26 (1H, t, J=6.8 Hz), 4.78-4.65 (2H, m), 4.01-3.95 (1H, m), 3.78 (6H, s), 3.55-3.40 (1H, m), 3.42 (1H, dd, J=10.5, 2.7 Hz), 3.40-3.28 (1H, m), 3.22 (1H, dd, J=10.5, 3.0 Hz), 3.19-3.06 (1H, m), 2.16-1.95 (2H, m), 1.90-1.54 (3H, m), 1.49-1.35 (1H, m), 1.43 (3H, s), 1.34-1.17 (2H, m), 0.67 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 156.2 (1P, s). Oligos were synthesized using the above compound 13b by the general method disclosed above. 
     Example Z-14 
     Oxazaphospholidine Monomer 14a 
     
       
         
         
             
             
         
       
     
     Compound 14a was obtained by using “5′-O-(DMTr)-4-N-(isobutyryl)cytidine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.33 (1H, brs), 8.17 (1H, d, J=7.5 Hz), 7.52-7.22 (19H, m), 7.07 (1H, d, J=7.5 Hz), 6.88-6.81 (4H, m), 6.20 (1H, t, J=6.2 Hz), 4.81-4.64 (2H, m), 3.93-3.87 (1H, m), 3.79 (6H, s), 3.59-3.43 (1H, m), 3.39-3.29 (3H, m), 3.16-3.02 (1H, m), 2.69-2.52 (2H, m), 2.12-2.00 (1H, m), 1.91-1.50 (3H, m), 1.47-1.32 (2H, m), 1.27-1.16 (7H, m), 0.60 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 154.8 (1P, s). 
     Example Z-16 
     Oxazaphospholidine Monomer 14b 
     
       
         
         
             
             
         
       
     
     Compound 14b was obtained by using 3b instead of 3a in a similar manner to compound 14a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.33 (1H, d, J=7.5 Hz), 8.23 (1H, brs), 7.57-7.22 (19H, m), 7.12 (1H, d, J=7.5 Hz), 6.88-6.81 (4H, m), 6.15 (1H, dd, J=6.6, 4.2 Hz), 4.82-4.63 (2H, m), 4.03-3.97 (1H, m), 3.80 (6H, s), 3.55-3.26 (4H, m), 3.19-3.05 (1H, m), 2.59 (1H, quintet, J=6.9 Hz), 2.39-2.27 (1H, m), 2.21-2.10 (1H, m), 1.90-1.56 (3H, m), 1.50-1.32 (2H, m), 1.26-1.17 (7H, m), 0.66 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 157.2 (1P, s). 
     Example Z-17 
     Oxazaphospholidine Monomer 15a 
     
       
         
         
             
             
         
       
     
     Compound 15a was obtained by using “5′-O-(DMTr)-6-N-(benzoyl)adenosine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a. 
       1 H NMR (600 MHz, CDCl 3 ) δ 8.71 (1H, s), 8.12 (1H, s), 8.04 (2H, d, J=7.8 Hz), 7.62-7.15 (23H, m), 6.80-6.75 (4H, m), 6.37 (1H, dd, J=7.8, 6.0 Hz), 4.94-4.88 (1H, m), 4.80 (1H, ddd, J=12.0, 6.0, 5.4 Hz), 4.07-4.04 (1H, m), 3.76 (6H, s), 3.58-3.49 (1H, m), 3.41-3.34 (1H, m), 3.33 (1H, dd, J=10.8, 4.8 Hz), 3.25 (1H, dd, J=10.8, 4.8 Hz), 3.13-3.06 (1H, m), 2.66-2.58 (1H, m), 2.40-2.35 (1H, m), 1.91-1.84 (1H, m), 1.73-1.66 (1H, m), 1.56 (1H, dd, J=15.0, 9.0 Hz), 1.44 (1H, dd, J=15.0, 5.4 Hz), 1.47-1.41 (1H, m), 1.30-1.23 (1H, m), 0.63 (3H, s);  31 P NMR (243.0 MHz, CDCl 3 ) δ 151.8 (1P, s). 
     Example Z-18 
     Oxazaphospholidine Monomer 15b 
     
       
         
         
             
             
         
       
     
     Compound 15b was obtained by using 3b instead of 3a in a similar manner to compound 15a.  1 H NMR (300 MHz, CDCl 3 ) δ 9.06 (1H, brs), 8.76 (1H, s), 8.12 (1H, s), 8.07-7.99 (2H, m), 7.64-7.14 (22H, m), 6.83-6.75 (4H, m), 6.25 (1H, t, J=6.6 Hz), 4.86-4.75 (2H, m), 4.20-4.15 (1H, m), 3.77 (6H, s), 3.61-3.38 (2H, m), 3.36 (1H, dd, J=10.2, 4.2 Hz), 3.27 (1H, dd, J=10.2, 4.2 Hz), 3.27-3.13 (1H, m), 2.71-2.59 (1H, m), 2.12-2.01 (1H, m), 1.94-1.42 (5H, m), 1.36-1.20 (1H, m), 0.67 (3H, s));  31 P NMR (121.5 MHz, CDCl 3 ) δ 157.3 (1P, s). 
     Example Z-19 
     Oxazaphospholidine Monomer 16a 
     
       
         
         
             
             
         
       
     
     Compound 16a was obtained by using 7a instead of 3a in a similar manner to compound 13a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.57 (1H, d, J=0.9 Hz), 7.37-6.94 (20H, m), 6.87-6.78 (4H, m), 6.48 (1H, dd, J=8.6, 5.7 Hz), 5.42 (1H, dd, J=11.0, 5.1 Hz), 4.81-4.71 (1H, m), 4.02 (1H, d, J=11.0 Hz), 3.83 (1H, d, J=2.1 Hz), 3.79 (6H, s), 3.61-3.41 (2H, m), 3.24-3.09 (1H, m), 3.16 (1H, dd, J=10.8, 2.4 Hz), 3.02 (1H, dd, J=10.8, 2.4 Hz), 2.54-2.44 (1H, m), 2.34-2.22 (1H, m), 1.94-1.79 (1H, m), 1.74-1.56 (1H, m), 1.38 (3H, s), 1.38-1.28 (2H, m);  31 P NMR (121.5 MHz, CDCl 3 ) δ 160.9 (1P, s). 
     Example Z-20 
     Oxazaphospholidine Monomer 16b 
     
       
         
         
             
             
         
       
     
     Compound 16b was obtained by using 3b instead of 3a in a similar manner to compound 16a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.57 (1H, d, J=1.5 Hz), 7.43-7.11 (20H, m), 6.85-6.78 (4H, m), 6.48 (1H, dd, J=7.5, 5.7 Hz), 5.58 (1H, dd, J=11.4, 5.1 Hz), 4.82-4.73 (1H, m), 4.17-4.02 (2H, m), 3.78 (6H, s), 3.56-3.40 (3H, m), 3.32 (1H, dd, J=10.7, 2.4 Hz), 3.22-3.07 (1H, m), 2.26-2.04 (2H, m), 1.95-1.81 (1H, m), 1.74-1.56 (1H, m), 1.40 (3H, d, J=1.5 Hz), 1.44-1.34 (2H, m);  31 P NMR (121.5 MHz, CDCl 3 ) δ 162.2 (1P, s). 
     Example Z-21 
     Oxazaphospholidine Monomer 17a 
     
       
         
         
             
             
         
       
     
     Compound 17a was obtained by using 9a instead of 3a in a similar manner to compound 13a.  1 H NMR (300 MHz, CDCl 3 ) δ 9.22 (1H, brs), 8.05-7.99 (2H, m), 7.52 (1H, d, J=1.2 Hz), 7.41-7.19 (11H, m), 6.87-6.79 (4H, m), 6.37 (1H, dd, J=8.4, 5.7 Hz), 4.88-4.75 (2H, m), 3.86-3.80 (1H, m), 3.79 (6H, d, J=1.2 Hz), 3.64-3.49 (2H, m), 3.27-3.12 (3H, m), 2.97 (2H, d, J=6.6 Hz), 2.51-2.41 (1H, m), 2.33-2.20 (1H, m), 2.03-1.75 (2H, m), 1.72-1.59 (1H, m), 1.46-1.36 (1H, m), 1.40 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 157.5 (1P, s). 
     Example Z-22 
     Oxazaphospholidine Monomer 17b 
     
       
         
         
             
             
         
       
     
     Compound 17b was obtained by using 9b instead of 9a in a similar manner to compound 17a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.67 (1H, brs), 8.18-8.11 (2H, m), 7.57 (1H, d, J=1.2 Hz), 7.47-7.22 (11H, m), 6.86-6.79 (4H, m), 6.29 (1H, t, J=6.6 Hz), 4.87 (1H, dt, J=7.5, 5.7 Hz), 4.80-4.72 (1H, m), 4.11-4.05 (1H, m), 3.79 (6H, s), 3.67-3.47 (2H, m), 3.43 (1H, dd, J=10.8, 2.7 Hz), 3.27 (1H, dd, J=10.8, 2.4 Hz), 3.25-3.13 (1H, m), 3.07-2.99 (2H, m), 2.19-2.12 (2H, m), 2.03-1.62 (3H, m), 1.46-1.30 (1H, m), 1.41 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 158.1 (1P, s). 
     Example Z-23 
     Oxazaphospholidine Monomer 18a 
     
       
         
         
             
             
         
       
     
     Compound 18a was obtained by using “5′-O-(DMTr)-2′-O-TOM-6-N-(acetyl)adenosine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.82 (1H, brs), 8.49 (1H, s), 8.10 (1H, s), 7.58-7.17 (19H, m), 6.83-6.73 (4H, m), 6.11 (1H, d, J=6.6 Hz), 5.15 (1H, dd, J=6.6, 5.4 Hz), 4.98-4.77 (4H, m), 4.18-4.11 (1H, m), 3.76 (6H, s), 3.59-3.25 (4H, m), 3.16-3.02 (1H, m), 2.62 (3H, s), 1.91-1.53 (3H, m), 1.49-1.18 (3H, m), 0.96-0.80 (3H, m), 0.90 (18H, s), 0.62 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 156.7 (1P, s). 
     Example Z-24 
     Oxazaphospholidine Monomer 18b 
     
       
         
         
             
             
         
       
     
     Compound 18b was obtained by using 3b instead of 3a in a similar manner to compound 18a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.56 (1H, brs), 8.55 (1H, s), 8.13 (1H, s), 7.57-7.17 (19H, m), 6.82-6.73 (4H, m), 6.16 (1H, d, J=5.7 Hz), 5.06 (1H, t, J=5.6 Hz), 4.93 (1H, d, J=5.1 Hz), 4.83 (1H, d, J=5.1 Hz), 4.81-4.69 (2H, m), 4.27-4.19 (1H, m), 3.76 (6H, s), 3.55-3.40 (2H, m), 3.33-3.16 (2H, m), 3.12-2.97 (1H, m), 2.63 (3H, s), 1.88-1.52 (3H, m), 1.45-1.16 (3H, m), 0.91-0.79 (3H, m), 0.86 (18H, s), 0.64 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 154.8 (1P, s). 
     Example Z-25 
     Oxazaphospholidine Monomer 19a 
     
       
         
         
             
             
         
       
     
     Compound 19a was obtained by using “5′-O-(DMTr)-2′-O-(methyl)uridine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.91 (1H, d, J=7.8 Hz), 7.58-7.20 (19H, m), 6.88-6.80 (4H, m), 5.96 (1H, d, J=3.3 Hz), 5.19 (1H, d, J=7.8 Hz), 4.88-4.78 (1H, m), 4.66-4.57 (1H, m), 4.03-3.95 (1H, m), 3.90-3.74 (1H, m), 3.78 (6H, s), 3.77-3.71 (1H, m), 3.58-3.29 (2H, m), 3.45 (3H, s), 3.13-2.82 (2H, m), 1.88-1.53 (3H, m), 1.49-1.16 (3H, m), 0.60 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 155.3 (1P, s). 
     Example Z-26 
     Oxazaphospholidine Monomer 19b 
     
       
         
         
             
             
         
       
     
     Compound 19b was obtained by using 3b instead of 3a in a similar manner to compound 19a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.10 (1H, d, J=8.4 Hz), 7.58-7.20 (19H, m), 6.87-6.79 (4H, m), 5.89 (1H, d, J=1.5 Hz), 5.21 (1H, d, J=8.4 Hz), 4.92-4.82 (1H, m), 4.73-4.63 (1H, m), 4.15-4.08 (1H, m), 3.89-3.73 (1H, m), 3.78 (6H, s), 3.66-3.62 (1H, m), 3.57-3.27 (2H, m), 3.30 (3H, s), 3.17-2.82 (2H, m), 1.89-1.55 (3H, m), 1.55-1.40 (1H, m), 1.35-1.15 (2H, m), 0.66 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 157.5 (1P, s). 
     Example Z-27 
     Oxazaphospholidine Monomer 20a 
     
       
         
         
             
             
         
       
     
     Compound 20a was obtained by using “5′-O-(DMTr)-2′-deoxy-2′-fluorouridine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.85 (1H, d, J=8.1 Hz), 7.58-7.20 (19H, m), 6.87-6.79 (4H, m), 5.98 (1H, d, J=16.5 Hz), 5.23 (1H, d, J=8.1 Hz), 4.86-4.61 (3H, m), 3.99 (1H, d, J=6.9 Hz), 3.76 (6H, d, J=3.0 Hz), 3.56-3.34 (4H, m), 3.10-2.96 (1H, m), 1.88-1.74 (1H, m), 1.72-1.52 (2H, m), 1.48-1.16 (3H, m), 0.61 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 154.3 (1P, d, J=8.9 Hz). 
     Example Z-28 
     Oxazaphospholidine Monomer 20b 
     
       
         
         
             
             
         
       
     
     Compound 20b was obtained by using 3b instead of 3a in a similar manner to compound 20a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.01 (1H, d, J=8.4 Hz), 7.58-7.20 (19H, m), 6.87-6.79 (4H, m), 6.03 (1H, d, J=16.2 Hz), 5.29 (1H, d, J=8.4 Hz), 4.96 (1H, dd, J=13.1, 7.5 Hz), 4.80-4.54 (2H, m), 4.15 (1H, d, J=9.0 Hz), 3.78 (6H, s), 3.61-3.39 (3H, m), 3.37-3.25 (1H, m), 3.23-3.09 (1H, m), 1.91-1.56 (3H, m), 1.51-1.13 (3H, m), 0.66 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 158.9 (1P, d, J=4.4 Hz). 
     Example Z-29 
     Oxazaphospholidine Monomer 21a 
     
       
         
         
             
             
         
       
     
     Compound 21a was obtained by using “5′-O-(DMTr)-2′-O-methoxyethyl-5-methyluridine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.62-7.18 (21H, m), 6.84 (4H, d, J=8.7 Hz), 6.07 (1H, d, J=5.7 Hz), 4.86-4.76 (1H, m), 4.63-4.54 (1H, m), 4.20 (1H, t, J=5.4 Hz), 3.95-3.89 (1H, m), 3.78 (6H, s), 3.78-3.71 (2H, m), 3.60-3.48 (2H, m), 3.44-3.02 (5H, m), 3.31 (3H, s), 1.88-1.15 (6H, m), 1.35 (3H, s), 0.58 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 156.3 (1P, s). 
     Example Z-30 
     Oxazaphospholidine Monomer 21b 
     
       
         
         
             
             
         
       
     
     Compound 21b was obtained by using 3b instead of 3a in a similar manner to compound 21a.  1 H NMR (300 MHz, CDCl 3 ) δ 7.71 (1H, d, J=1.2 Hz), 7.55-7.22 (20H, m), 6.86-6.78 (4H, m), 5.99 (1H, d, J=3.9 Hz), 4.78-4.62 (2H, m), 4.13-4.08 (1H, m), 4.07-4.02 (1H, m), 3.77 (6H, s), 3.77-3.70 (1H, m), 3.65-3.56 (1H, m), 3.52-3.36 (4H, m), 3.33-3.14 (2H, m), 3.29 (3H, s), 3.08-2.94 (1H, m), 1.86-1.72 (1H, m), 1.71-1.55 (2H, m), 1.30 (3H, d, J=1.2 Hz), 1.47-1.16 (3H, m) 0.64 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 155.6 (1P, s). 
     Example Z-31 
     Oxazaphospholidine Monomer 22a 
     
       
         
         
             
             
         
       
     
     Compound 22a was obtained by using “5′-O-(DMTr)-2′-O-methyl-4-N-(isobutyryl)cytidine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.49 (1H, d, J=7.2 Hz), 7.58-7.20 (19H, m), 6.96 (1H, d, J=7.2 Hz), 6.90-6.82 (4H, m), 5.98 (1H, s), 4.84 (1H, dd, J=13.1, 7.5 Hz), 4.59 (1H, dt, J=8.3, 4.5 Hz), 4.19-4.13 (1H, m), 3.79 (6H, s), 3.78-3.72 (1H, m), 3.63-3.40 (3H, m), 3.55 (3H, s), 3.36-3.24 (1H, m), 3.09-2.95 (1H, m), 2.59 (1H, septet, J=6.9 Hz), 1.85-1.53 (5H, m), 1.48-1.37 (1H, m), 1.24-1.17 (6H, m), 0.59 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 155.2 (1P, s). 
     Example Z-32 
     Oxazaphospholidine Monomer 22b 
     
       
         
         
             
             
         
       
     
     Compound 22b was obtained by using 3b instead of 3a in a similar manner to compound 22a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.62 (1H, d, J=7.5 Hz), 7.57-7.23 (19H, m), 7.02 (1H, d, J=7.5 Hz), 6.89-6.81 (4H, m), 5.92 (1H, s), 4.90 (1H, dt, J=9.0, 5.7 Hz), 4.61 (1H, dt, J=8.7, 4.8 Hz), 4.25-4.17 (1H, m), 3.81 (6H, s), 3.67 (1H, d, J=4.5 Hz), 3.62-3.25 (4H, m), 3.38 (3H, s), 3.16-3.02 (1H, m), 2.58 (1H, septet, J=6.9 Hz), 1.87-1.40 (6H, m), 1.26-1.14 (6H, m), 0.64 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 158.2 (1P, s). 
     Example Z-33 
     Oxazaphospholidine Monomer 23a 
     
       
         
         
             
             
         
       
     
     Compound 23a was obtained by using “5′-O-(DMTr)-2′-O-methyl-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.67 (1H, brs), 8.01 (1H, s), 7.56-7.16 (24H, m), 6.83-6.74 (4H, m), 6.08 (1H, d, J=6.9 Hz), 4.85-4.76 (1H, m), 4.84 (2H, t, J=6.6 Hz), 4.65-4.56 (1H, m), 4.59 (2H, brs), 4.48 (1H, dd, J=6.6, 5.1 Hz), 4.09-4.05 (1H, m), 3.75 (6H, s), 3.60-3.42 (2H, m), 3.40-3.26 (2H, m), 3.35 (3H, s), 3.18-3.05 (1H, m), 3.08 (2H, t, J=6.6 Hz), 1.89-1.49 (3H, m), 1.48-1.16 (3H, m), 0.59 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 156.9 (1P, s). 
     Example Z-34 
     Oxazaphospholidine Monomer 23b 
     
       
         
         
             
             
         
       
     
     Compound 23b was obtained by using 3b instead of 3a in a similar manner to compound 23a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.74 (1H, brs), 8.09 (1H, s), 7.56-6.94 (24H, m), 6.84-6.71 (4H, m), 6.09 (1H, d, J=4.8 Hz), 4.83-4.70 (2H, m), 4.83 (2H, t, J=6.6 Hz), 4.63 (2H, brs), 4.35 (1H, t, J=5.0 Hz), 4.23-4.16 (1H, m), 3.75 (6H, s), 3.58-3.19 (4H, m), 3.32 (3H, s), 3.16-3.04 (1H, m), 3.07 (2H, t, J=6.6 Hz), 1.90-1.55 (3H, m), 1.48-1.15 (3H, m), 0.64 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 154.6 (1P, s). 
     Example Z-35 
     Oxazaphospholidine Monomer 24a 
     
       
         
         
             
             
         
       
     
     Compound 24a was obtained by using “5′-O-(DMTr)-2′-deoxy-2′-fluoro-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.74 (1H, brs), 8.03 (1H, s), 7.55-6.94 (24H, m), 6.80-6.69 (4H, m), 6.21 (1H, dd, J=14.9, 3.6 Hz), 5.34 (1H, dt, J=52.3, 3.6 Hz), 5.01-4.75 (2H, m), 4.84 (1H, t, J=6.6 Hz), 4.62 (2H, brs), 4.15-4.07 (1H, m), 3.73 (6H, s), 3.59-3.29 (4H, m), 3.15-3.00 (1H, m), 3.07 (2H, t, J=6.6 Hz), 1.90-1.49 (3H, m), 1.47-1.12 (3H, m), 0.58 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 155.6 (1P, d, J=10.9 Hz). 
     Example Z-36 
     Oxazaphospholidine Monomer 24b 
     
       
         
         
             
             
         
       
     
     Compound 24b was obtained by using 3b instead of 3a in a similar manner to compound 24a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.81 (1H, brs), 8.06 (1H, s), 7.55-6.95 (24H, m), 6.77-6.69 (4H, m), 6.06 (1H, d, J=17.1 Hz), 5.24-5.08 (1H, m), 5.04-4.80 (2H, m), 4.87 (1H, t, J=6.6 Hz), 4.62 (2H, brs), 4.25-4.19 (1H, m), 3.73 (6H, s), 3.58-3.02 (5H, m), 3.10 (2H, t, J=6.6 Hz), 1.90-1.56 (3H, m), 1.50-1.15 (3H, m), 0.63 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 158.0 (1P, d, J=4.4 Hz). 
     Example Z-37 
     Oxazaphospholidine Monomer 25a 
     
       
         
         
             
             
         
       
     
     Compound 25a was obtained by using “5′-O-(DMTr)-2′-O-TOM-4-N-(acetyl)cytidine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 10.04 (1H, brs), 8.30 (1H, d, J=7.5 Hz), 7.51-7.21 (19H, m), 6.99 (1H, d, J=7.5 Hz), 6.89-6.81 (4H, m), 6.12 (1H, d, J=3.3 Hz), 5.07 (1H, d, J=4.8 Hz), 5.05 (1H, d, J=4.8 Hz), 4.84-4.75 (1H, m), 4.62-4.52 (1H, m), 4.31-4.25 (1H, m), 4.08-4.01 (1H, m), 3.78 (6H, d, J=3.0 Hz), 3.55-3.23 (4H, m), 3.10-2.96 (1H, m), 2.24 (3H, s), 1.84-1.49 (3H, m), 1.46-0.96 (24H, m), 0.58 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 156.5 (1P, s). 
     Example Z-38 
     Oxazaphospholidine Monomer 25b 
     
       
         
         
             
             
         
       
     
     Compound 25b was obtained by using 3b instead of 3a in a similar manner to compound 25a.  1 H NMR (300 MHz, CDCl 3 ) δ 10.19 (1H, brs), 8.46 (1H, d, J=7.5 Hz), 7.54-7.23 (19H, m), 7.01 (1H, d, J=7.5 Hz), 6.88-6.79 (4H, m), 6.19 (1H, d, J=1.8 Hz), 5.11 (1H, d, J=4.8 Hz), 5.07 (1H, d, J=4.8 Hz), 4.81-4.71 (1H, m), 4.60-4.51 (1H, m), 4.26-4.18 (2H, m), 3.79 (6H, s), 3.63-3.55 (1H, m), 3.48-3.28 (2H, m), 3.21-2.94 (2H, m), 2.26 (3H, s), 1.81-1.49 (3H, m), 1.43-0.96 (24H, m), 0.62 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 156.4 (1P, s). 
     Example Z-39 
     Oxazaphospholidine Monomer 26a 
     
       
         
         
             
             
         
       
     
     Compound 26a was obtained by using “5′-O-(DMTr)-2′-deoxy-2′-fluoro-4-N-(isobutyryl)cytidine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.66 (1H, brs), 8.41 (1H, d, J=7.5 Hz), 7.55-7.20 (19H, m), 7.01 (1H, d, J=7.5 Hz), 6.89-6.81 (4H, m), 6.06 (1H, d, J=15.9 Hz), 4.85 (1H, dd, J=51.4, 3.9 Hz), 4.84 (1H, dd, J=12.9, 7.5 Hz), 4.77-4.59 (1H, m), 4.15-4.08 (1H, m), 3.79 (6H, s), 3.63-3.29 (4H, m), 3.10-2.96 (1H, m), 2.65 (1H, septet, J=6.9 Hz), 1.85-1.53 (3H, m), 1.48-1.17 (3H, m), 1.21 (3H, d, J=4.8 Hz), 1.19 (3H, d, J=4.8 Hz), 0.59 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 155.5 (1P, d, J=6.6 Hz). 
     Example Z-40 
     Oxazaphospholidine Monomer 26b 
     
       
         
         
             
             
         
       
     
     Compound 26b was obtained by using 3b instead of 3a in a similar manner to compound 26a.  1 H NMR (300 MHz, CDCl 3 ) δ 8.53 (1H, d, J=7.5 Hz), 7.57-7.23 (20H, m), 7.10 (11H, d, J=7.5 Hz), 6.89-6.81 (4H, m), 6.10 (1H, d, J=15.9 Hz), 5.00-4.92 (1H, m), 4.84 (1H, dd, J=51.5, 3.3 Hz), 4.75-4.58 (1H, m), 4.24 (1H, d, J=9.3 Hz), 3.81 (6H, s), 3.65-3.39 (3H, m), 3.32-3.06 (2H, m), 2.59 (1H, septet, J=6.9 Hz), 1.88-1.53 (4H, m), 1.49-1.34 (2H, m), 1.27-1.18 (6H, m), 0.65 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 159.0 (1P, d, J=4.4). 
     Oxazaphospholidine Monomer 27a 
     
       
         
         
             
             
         
       
     
     Compound 27a was obtained by using “5′-O-(DMTr)-2′-O-methyl-6-N-(benzoyl)adenosine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 8.66 (1H, s), 8.13 (1H, s), 8.03 (2H, d, J=7.2 Hz), 7.64-7.16 (23H, m), 6.79 (4H, d, J=8.7 Hz), 6.08 (1H, d, J=6.3 Hz), 4.91-4.81 (1H, m), 4.77-4.69 (1H, m), 4.64-4.57 (1H, m), 4.15-4.10 (1H, m), 3.76 (6H, s), 3.60-3.23 (4H, m), 3.35 (3H, s), 3.14-3.00 (1H, m), 1.90-1.19 (6H, m), 0.62 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 155.8 (1P, s). 
     Oxazaphospholidine Monomer 27b 
     
       
         
         
             
             
         
       
     
     Compound 27b was obtained by using 3b instead of 3a in a similar manner to compound 27a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 9.12 (1H, brs), 8.73 (1H, s), 8.24 (1H, s), 8.07-8.01 (2H, m), 7.62-7.17 (22H, m), 6.83-6.77 (4H, m), 6.12 (1H, d, J=4.8 Hz), 4.84-4.73 (2H, m), 4.43 (1H, t, J=4.8 Hz), 4.25-4.19 (1H, m), 3.77 (6H, s), 3.55-3.20 (4H, m), 3.28 (3H, s), 3.16-3.03 (1H, m), 1.90-1.17 (6H, m), 0.65 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 155.0 (1P, s). 
     Oxazaphospholidine Monomer 28a 
     
       
         
         
             
             
         
       
     
     Compound 28a was obtained by using “5′-O-(DMTr)-2′-deoxy-2′-fluoro-6-N-(benzoyl)adenosine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 8.64 (1H, s), 8.14 (1H, s), 8.06-8.01 (2H, m), 7.63-7.07 (23H, m), 6.78-6.70 (4H, m), 6.12 (1H, dd, J=18.0, 2.4 Hz), 5.24-5.01 (2H, m), 4.94-4.84 (1H, m), 4.17-4.06 (1H, m), 3.73 (6H, s), 3.55-3.40 (3H, m), 3.30-3.22 (1H, m), 3.03-2.88 (1H, m), 1.92-1.19 (6H, m), 0.62 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 150.5 (1P, d, J=7.7 Hz). 
     Oxazaphospholidine Monomer 28b 
     
       
         
         
             
             
         
       
     
     Compound 28b was obtained by using 3b instead of 3a in a similar manner to compound 28a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 9.07 (1H, brs), 8.80 (1H, s), 8.24 (1H, s), 8.08-8.01 (2H, m), 7.66-7.15 (22H, m), 6.81-6.75 (4H, m), 6.14 (1H, dd, J=18.0, 1.8 Hz), 5.16-4.91 (3H, m), 4.28-4.21 (1H, m), 3.76 (6H, s), 3.57-3.11 (5H, m), 1.82-1.16 (6H, m), 0.65 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 157.8 (1P, d, J=5.6 Hz). 
     Oxazaphospholidine Monomer 29a 
     
       
         
         
             
             
         
       
     
     Compound 29a was obtained by using “5′-O-(DMTr)-2′-O-TOM-2-N-(acetyl)guanosine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 7.70 (1H, s), 7.63-7.13 (21H, m), 6.84-6.76 (4H, m), 5.77 (1H, d, J=8.4 Hz), 5.41-5.33 (1H, m), 4.90 (2H, s), 4.78-4.68 (2H, m), 3.86 (1H, brs), 3.75 (3H, s), 3.74 (3H, s), 3.56-3.41 (2H, m), 3.32-2.90 (3H, m), 1.92-1.10 (9H, m), 0.97-0.87 (21H, m), 0.52 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 158.1 (1P, s). 
     Oxazaphospholidine Monomer 29b 
     
       
         
         
             
             
         
       
     
     Compound 29b was obtained by using 3b instead of 3a in a similar manner to compound 29a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 7.77 (1H, s), 7.56-7.15 (21H, m), 6.82-6.75 (4H, m), 5.86 (1H, d, J=7.5 Hz), 5.26-5.17 (1H, m), 4.95 (1H, d, J=5.4 Hz), 4.85 (1H, d, J=5.4 Hz), 4.78-4.71 (1H, m), 4.59-4.49 (1H, m), 4.10-4.05 (1H, m), 3.74 (6H, s), 3.52-3.37 (2H, m), 3.30-3.18 (1H, m), 3.11-2.85 (2H, m), 1.85-1.15 (9H, m), 0.93-0.84 (21H, m), 0.62 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 152.3 (1P, s). 
     Oxazaphospholidine Monomer 30a 
     
       
         
         
             
             
         
       
     
     Compound 30a was obtained by using “5′-O-(DMTr)-2′-O-TOM-uridine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 7.76 (1H, d, J=8.1 Hz), 7.55-7.18 (20H, m), 6.88-6.80 (4H, m), 6.11 (1H, d, J=6.0 Hz), 5.32 (1H, d, J=8.1 Hz), 4.99 (1H, d, J=5.1 Hz), 4.93 (1H, d, J=5.1 Hz), 4.84-4.75 (1H, m), 4.54-4.46 (1H, m), 4.38 (1H, t, J=5.7 Hz), 3.87-3.83 (1H, m), 3.78 (3H, s), 3.77 (3H, s), 3.56-3.42 (1H, m), 3.39-3.28 (1H, m), 3.36 (1H, dd, J=11.0, 2.7 Hz), 3.25 (1H, dd, J=11.0, 2.7 Hz), 3.16-3.03 (1H, m), 1.88-1.12 (6H, m), 1.08-0.97 (21H, m), 0.59 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 156.6 (1P, s). 
     Oxazaphospholidine Monomer 30b 
     
       
         
         
             
             
         
       
     
     Compound 30b was obtained by using 3b instead of 3a in a similar manner to compound 30a. 
       1 H NMR (600 MHz, CDCl 3 ) δ 7.87 (1H, d, J=7.8 Hz), 7.52-7.48 (4H, m), 7.38-7.21 (16H, m), 6.83-6.79 (4H, m), 6.14 (1H, d, J=4.8 Hz), 5.33 (1H, d, J=7.8 Hz), 4.99 (1H, d, J=5.4 Hz), 4.89 (1H, d, J=5.4 Hz), 4.67 (1H, dd, J=13.8, 7.2 Hz), 4.52 (1H, dt, J=10.4, 4.8 Hz), 4.31 (1H, t, J=4.8 Hz), 4.06-4.03 (1H, m), 3.78 (3H, s), 3.77 (3H, s), 3.47 (1H, dd, J=10.4, 2.4 Hz), 3.47-3.39 (1H, m), 3.22-3.17 (2H, m), 3.00 (1H, ddd, J=19.5, 10.4, 4.8 Hz), 1.82-1.74 (1H, m), 1.68-1.58 (1H, m), 1.56 (1H, dd, J=14.4, 8.4 Hz), 1.38 (1H, dd, J=14.4, 7.2 Hz), 1.31-1.25 (1H, m), 1.26-1.17 (1H, m), 1.08-0.98 (21H, m), 0.63 (3H, s);  31 P NMR (243.0 MHz, CDCl 3 ) δ 154.3 (1P, s). 
     Oxazaphospholidine Monomer 31a 
     
       
         
         
             
             
         
       
     
     Compound 31a was obtained by using “5′-O-(DMTr)-2′-O,4′-C-methylene-6-N-(benzoyl)adenosine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 9.10 (1H, brs), 8.76 (1H, s), 8.32 (1H, s), 8.04 (2H, d, J=7.2 Hz), 7.64-7.18 (22H, m), 6.84 (4H, d, J=8.7 Hz), 6.10 (1H, s), 4.76 (1H, d J=6.9 Hz), 4.58 (1H, s), 4.61-4.51 (1H, m), 3.91 (1H, d, J=7.8 Hz), 3.77 (1H, d, J=7.8 Hz), 3.75 (6H, s), 3.50 (1H, s), 3.47-3.33 (1H, m), 3.31-3.19 (1H, m), 3.03-2.88 (1H, m), 1.84-1.09 (6H, m), 0.51 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 152.9 (1P, s). 
     Oxazaphospholidine Monomer 31b 
     
       
         
         
             
             
         
       
     
     Compound 31b was obtained by using 3b instead of 3a in a similar manner to compound 31a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 8.81 (1H, s), 8.30 (1H, s), 8.07-8.00 (2H, m), 7.64-7.17 (22H, m), 6.86-6.79 (4H, m), 6.12 (1H, s), 4.81-4.72 (1H, m), 4.62 (1H, d J=7.2 Hz), 4.57 (1H, s), 3.94 (1H, d, J=7.8 Hz), 3.89 (1H, d, J=7.8 Hz), 3.77 (6H, s), 3.48 (2H, s), 3.46-3.32 (1H, m), 3.24-3.13 (1H, m), 3.10-2.97 (1H, m), 1.84-1.49 (3H, m), 1.42-1.09 (3H, m), 0.58 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 157.3 (1P, s). 
     Oxazaphospholidine Monomer 32a 
     
       
         
         
             
             
         
       
     
     Compound 32a was obtained by using “5′-O-(DMTr)-2′-O,4′-C-methylene-4-N-(isobutyryl)-5-methylcytidine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 7.88 (1H, brs), 7.58-7.18 (20H, m), 6.88-6.80 (4H, m), 5.65 (1H, s), 4.69-4.60 (1H, m), 4.52 (1H, d, J=6.6 Hz), 4.49 (1H, s), 3.81-3.74 (1H, m), 3.75 (3H, s), 3.73 (3H, s), 3.64 (1H, d, J=8.1 Hz), 3.56 (1H, d, J=11.1 Hz), 3.53 (1H, d, J=8.1 Hz), 3.46 (1H, d, J=11.1 Hz), 3.56-3.40 (1H, m), 3.32-3.20 (1H, m), 3.14-3.00 (1H, m), 1.85-1.12 (6H, m), 1.60 (3H, s), 1.19 (6H, d, J=6.9 Hz), 0.55 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 155.9 (1P, s). 
     Oxazaphospholidine Monomer 32b 
     
       
         
         
             
             
         
       
     
     Compound 32b was obtained by using 3b instead of 3a in a similar manner to compound 32a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 7.86 (1H, brs), 7.56-7.19 (20H, m), 6.88-6.79 (4H, m), 5.69 (1H, s), 4.86-4.76 (1H, m), 4.46 (1H, s), 4.45 (1H, d, J=7.5 Hz), 3.80-3.75 (1H, m), 3.79 (6H, s), 3.74 (1H, d, J=8.1 Hz), 3.69 (1H, d, J=8.1 Hz), 3.51 (1H, d, J=11.1 Hz), 3.44-3.30 (1H, m), 3.39 (1H, d, J=11.1 Hz), 3.29-3.17 (1H, m), 3.11-2.97 (1H, m), 1.86-1.52 (3H, m), 1.64 (3H, s), 1.45-1.10 (3H, m), 1.21 (6H, d, J=6.6 Hz), 0.62 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 158.2 (1P, s). 
     Oxazaphospholidine Monomer 33a 
     
       
         
         
             
             
         
       
     
     Compound 33a was obtained by using “5′-O-(DMTr)-2′-O,4′-C-methylene-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 8.71 (1H, brs), 8.16 (1H, s), 7.50-7.17 (21H, m), 7.09-7.01 (3H, m), 6.86-6.79 (4H, m), 6.03 (1H, s), 4.84 (2H, t, J=6.6 Hz), 4.72 (2H, s), 4.68 (1H, d, J=7.2 Hz), 4.55-4.46 (1H, m), 4.50 (1H, s), 3.90 (1H, d, J=7.8 Hz), 3.77 (1H, d, J=7.8 Hz), 3.75 (6H, s), 3.51 (1H, d, J=10.8 Hz), 3.47 (1H, d, J=10.8 Hz), 3.45-3.21 (2H, m), 3.08 (2H, t, J=6.6 Hz), 3.03-2.89 (1H, m), 1.80-1.08 (6H, m), 0.47 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 153.2 (1P, s). 
     Oxazaphospholidine Monomer 33b 
     
       
         
         
             
             
         
       
     
     Compound 33b was obtained by using 3b instead of 3a in a similar manner to compound 33a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 8.86 (1H, brs), 8.13 (1H, s), 7.55-7.17 (21H, m), 7.08-6.98 (3H, m), 6.95-6.78 (4H, m), 6.01 (1H, s), 4.86 (2H, t, J=6.6 Hz), 4.82-4.73 (1H, m), 4.70 (2H, s), 4.64 (1H, d, J=7.5 Hz), 4.49 (1H, s), 3.94 (1H, d, J=7.8 Hz), 3.89 (1H, d, J=7.8 Hz), 3.77 (6H, s), 3.46 (2H, s), 3.45-3.30 (1H, m), 3.24-3.12 (1H, m), 3.09 (2H, t, J=6.6 Hz), 3.09-2.96 (1H, m), 1.81-1.50 (3H, m), 1.41-1.06 (3H, m), 0.58 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 157.4 (1P, s). 
     Oxazaphospholidine Monomer 34a 
     
       
         
         
             
             
         
       
     
     Compound 34a was obtained by using “5′-O-(DMTr)-2′-O,4′-C-methylene-5-methyluridine” instead of “5′-O-(DMTr)-2-N-(phenoxyacetyl)-6-O-(cyanoethyl)guanosine” in a similar manner to compound 12a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 7.71 (1H, d, J=0.9 Hz), 7.50-7.17 (20H, m), 6.87-6.80 (4H, m), 5.61 (1H, s), 4.69-4.60 (1H, m), 4.55 (1H, d, J=6.9 Hz), 4.41 (1H, s), 3.74 (3H, s), 3.73 (3H, s), 3.64 (1H, d, J=7.8 Hz), 3.55 (1H, d, J=7.8 Hz), 3.53 (1H, d, J=10.8 Hz), 3.46 (1H, d, J=10.8 Hz), 3.56-3.42 (1H, m), 3.35-3.24 (1H, m), 3.13-3.00 (1H, m), 1.85-1.45 (3H, m), 1.55 (3H, d, J=0.9 Hz), 1.41-1.12 (3H, m), 0.56 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 155.1 (1P, s). 
     Oxazaphospholidine Monomer 34b 
     
       
         
         
             
             
         
       
     
     Compound 34b was obtained by using 3b instead of 3a in a similar manner to compound 34a. 
       1 H NMR (300 MHz, CDCl 3 ) δ 7.69 (1H, s), 7.56-7.19 (20H, m), 6.88-6.79 (4H, m), 5.66 (1H, s), 4.87-4.77 (1H, m), 4.47 (1H, d, J=7.8 Hz), 4.40 (1H, s), 3.78 (6H, s), 3.74 (1H, d, J=7.8 Hz), 3.68 (1H, d, J=7.8 Hz), 3.50 (1H, d, J=10.8 Hz), 3.46-3.32 (1H, m), 3.39 (1H, d, J=10.8 Hz), 3.30-3.19 (1H, m), 3.12-2.98 (1H, m), 1.85-1.56 (3H, m), 1.59 (31, s), 1.46-1.12 (3H, m), 0.63 (3H, s);  31 P NMR (121.5 MHz, CDCl 3 ) δ 158.1 (1P, s). 
     Oxazaphospholidine Monomer 35a 
     
       
         
         
             
             
         
       
     
     Compound 35a was obtained by using 13a′ instead of 3a in a similar manner to compound 13a.  1 H NMR (600 MHz, CDCl 3 ) δ 7.76 (2H, d, J=9.0 Hz), 7.62 (1H, d, J=1.2 Hz), 7.40 (2H, d, J=7.2 Hz), 7.32-7.23 (101, m), 6.85 (4H, d, J=8.4 Hz), 6.41 (1H, dd, J=8.4, 5.4 Hz), 4.94 (1H, dd, J=12.3, 5.4 Hz), 4.84-4.79 (1H, m), 4.03-4.01 (1H, m), 3.79 (6H, s), 3.59-3.53 (1H, m), 3.52-3.44 (2H, m), 3.41 (1H, dd, J=14.7, 7.2 Hz), 3.37-3.30 (2H, m), 3.13 (1H, ddd, J=19.3, 10.3, 4.1 Hz), 2.50-2.44 (1H, m), 2.39 (3H, s), 2.35-2.29 (1H, m), 1.91-1.72 (2H, m), 1.64-1.59 (1H, m), 1.40 (3H, s), 1.12-1.05 (1H, m);  31 P NMR (243.0 MHz, CDCl 3 ) δ 154.2 (1P, s). 
     Example Z-41 
     
       
         
         
             
             
         
       
     
     The above Compound Z-27, which represents a conventional monomer, was used to produce oligos.  FIG.  70    shows a chart of products obtained through Comparison Example Z-1. As shown in  FIGS.  69  and  70   , the present monomers provided more complete deprotection and less side product, which makes product isolation and/or purification easier. 
     In some embodiments, the present invention provides chemically stable monomers. Exemplary such monomers are depicted in the Examples above. In some embodiments, the present invention provides monomers with high isolated yield. In some embodiments, the present invention provides monomers with isolated yield higher than conventional method. In some embodiments, the isolated yield is more than 80%. Exemplary such monomers are depicted in the Examples above. 
     Condensing Reagent 
     Condensing reagents (C R ) useful in accordance with methods of the present invention are of any one of the following general formulae: 
                         
wherein Z 1 , Z 2 , Z 3 , Z 4 , Z 5 , Z 6 , Z 7 , Z 8 , and Z 9  are independently optionally substituted group selected from alkyl, aminoalkyl, cycloalkyl, heterocyclic, cycloalkylalkyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, or heteroaryloxy, or wherein any of Z 2  and Z 3 , Z 5  and Z 6 , Z 7  and Z 8 , Z 8  and Z 9 , Z 9  and Z 7 , or Z 7  and Z 8  and Z 9  are taken together to form a 3 to 20 membered alicyclic or heterocyclic ring; Q −  is a counter anion; and LG is a leaving group.
 
     In some embodiments, a counter ion of a condensing reagent C R  is Cl − , Br − , BF 4   − , PF 6   − , TfO − , Tf 2 N − , AsF 6   − , ClO 4   − , or SbF 6   − , wherein Tf is CF 3 SO 2 . In some embodiments, a leaving group of a condensing reagent C R  is F, Cl, Br, I, 3-nitro-1,2,4-triazole, imidazole, alkyltriazole, tetrazole, pentafluorobenzene, or 1-hydroxybenzotriazole. 
     Examples of condensing reagents used in accordance with methods of the present invention include, but are not limited to, pentafluorobenzoyl chloride, carbonyldiimidazole (CDI), 1-mesitylenesulfonyl-3-nitrotriazole (MSNT), 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide hydrochloride (EDCI-HCl), benzotriazole-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate (PyBOP), N,N′-bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BopCl), 2-(1H-7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU), and O-benzotriazole-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU), DIPCDI; N,N′-bis(2-oxo-3-oxazolidinyl)phosphinic bromide (BopBr), 1,3-dimethyl-2-(3-nitro-1,2,4-triazol-1-yl)-2-pyrrolidin-1-yl-1,3,2-diazaphospholidinium hexafluorophosphate (MNTP), 3-nitro-1,2,4-triazol-1-yl-tris(pyrrolidin-1-yl)phosphonium hexafluorophosphate (PyNTP), bromotripyrrolidinophosphonium hexafluorophosphate (PyBrOP); O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium tetrafluoroborate (TBTU); and tetramethylfluoroformamidinium hexafluorophosphate (TFFH). In certain embodiments, a counter ion of the condensing reagent C R  is Cl − , Br − , BF 4   − , PF 6   − , TfO − , Tf 2 N − , AsF 6   − , ClO 4   − , or SbF 6   − , wherein Tf is CF 3 SO 2 . 
     In some embodiments, a condensing reagent is 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl) tetrazolide, pivaloyl chloride, bromotrispyrrolidinophosphonium hexafluorophosphate, N,N′-bis(2-oxo-3-oxazolidinyl) phosphinic chloride (BopCl), or 2-chloro-5,5-dimethyl-2-oxo-1,3,2-dioxaphosphinane. In some embodiment, a condensing reagent is N,N′-bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BopCl). In some embodiments, a condensing reagent is selected from those described in WO/2006/066260). 
     In some embodiments, a condensing reagent is 1,3-dimethyl-2-(3-nitro-1,2,4-triazol-1-yl)-2-pyrrolidin-1-yl-1,3,2-diazaphospholidinium hexafluorophosphate (MNTP), or 3-nitro-1,2,4-triazol-1-yl-tris(pyrrolidin-1-yl)phosphonium hexafluorophosphate (PyNTP): 
                         
Selection of Base and Sugar of Nucleoside Coupling Partner
 
     As described herein, nucleoside coupling partners for use in accordance with methods of the present invention can be the same as one another or can be different from one another. In some embodiments, nucleoside coupling partners for use in the synthesis of a provided oligonucleotide are of the same structure and/or stereochemical configuration as one another. In some embodiments, each nucleoside coupling partner for use in the synthesis of a provided oligonucleotide is not of the same structure and/or stereochemical configuration as certain other nucleoside coupling partners of the oligonucleotide. Exemplary nucleobases and sugars for use in accordance with methods of the present invention are described herein. One of skill in the relevant chemical and synthetic arts will recognize that any combination of nucleobases and sugars described herein are contemplated for use in accordance with methods of the present invention. 
     Coupling Step: 
     Exemplary coupling procedures and chiral reagents and condensing reagents for use in accordance with the present invention are outlined in, inter alia, Wada I (JP4348077; WO2005/014609; WO2005/092909), Wada II (WO2010/064146), and Wada III (WO2012/039448). Chiral nucleoside coupling partners for use in accordance with the present invention are also referred to herein as “Wada amidites.” In some embodiments, a coupling partner has the structure of 
                         
wherein B PRO  is a protected nucleobase. In some embodiments, a coupling partner has the structure of
 
                         
wherein B PRO  is a protected nucleobase. Exemplary chiral phosphoramidites as coupling partner are depicted below:
 
     
       
         
         
             
             
         
       
     
     One of the methods used for synthesizing the coupling partner is depicted in Scheme II below. 
     
       
         
         
             
             
         
       
     
     In some embodiments, the step of coupling comprises reacting a free hydroxyl group of a nucleotide unit of an oligonucleotide with a nucleoside coupling partner under suitable conditions to effect the coupling. In some embodiments, the step of coupling is preceded by a step of deblocking. For instance, in some embodiments, the 5′ hydroxyl group of the growing oligonucleotide is blocked (i.e., protected) and must be deblocked in order to subsequently react with a nucleoside coupling partner. 
     Once the appropriate hydroxyl group of the growing oligonucleotide has been deblocked, the support is washed and dried in preparation for delivery of a solution comprising a chiral reagent and a solution comprising an activator. In some embodiments, a chiral reagent and an activator are delivered simultaneously. In some embodiments, co-delivery comprises delivering an amount of a chiral reagent in solution (e.g., a phosphoramidite solution) and an amount of activator in a solution (e.g., a CMPT solution) in a polar aprotic solvent such as a nitrile solvent (e.g., acetonitrile). 
     In some embodiments, the step of coupling provides a crude product composition in which the chiral phosphite product is present in a diastereomeric excess of &gt;95%. In some embodiments, the chiral phosphite product is present in a diastereomeric excess of &gt;96%. In some embodiments, the chiral phosphite product is present in a diastereomeric excess of &gt;97%. In some embodiments, the chiral phosphite product is present in a diastereomeric excess of &gt;98%. In some embodiments, the chiral phosphite product is present in a diastereomeric excess of &gt;99%. 
     Capping Step: 
     Provided methods for making chirally controlled oligonucleotides comprise a step of capping. In some embodiments, a step of capping is a single step. In some embodiments, a step of capping is two steps. In some embodiments, a step of capping is more than two steps. 
     In some embodiments, a step of capping comprises steps of capping the free amine of the chiral auxiliary and capping any residual unreacted 5′ hydroxyl groups. In some embodiments, the free amine of the chiral auxiliary and the unreacted 5′ hydroxyl groups are capped with the same capping group. In some embodiments, the free amine of the chiral auxiliary and the unreacted 5′ hydroxyl groups are capped with different capping groups. In certain embodiments, capping with different capping groups allows for selective removal of one capping group over the other during synthesis of the oligonucleotide. In some embodiments, the capping of both groups occurs simultaneously. In some embodiments, the capping of both groups occurs iteratively. 
     In certain embodiments, capping occurs iteratively and comprises a first step of capping the free amine followed by a second step of capping the free 5′ hydroxyl group, wherein both the free amine and the 5′ hydroxyl group are capped with the same capping group. For instance, in some embodiments, the free amine of the chiral auxiliary is capped using an anhydride (e.g., phenoxyacetic anhydride, i.e., Pac 2 O) prior to capping of the 5′ hydroxyl group with the same anhydride. In certain embodiments, the capping of the 5′ hydroxyl group with the same anhydride occurs under different conditions (e.g., in the presence of one or more additional reagents). In some embodiments, capping of the 5′ hydroxyl group occurs in the presence of an amine base in an etherial solvent (e.g., NMI (N-methylimidazole) in THF). The phrase “capping group” is used interchangeably herein with the phrases “protecting group” and “blocking group”. 
     In some embodiments, an amine capping group is characterized in that it effectively caps the amine such that it prevents rearrangement and/or decomposition of the intermediate phosphite species. In some embodiments, a capping group is selected for its ability to protect the amine of the chiral auxiliary in order to prevent intramolecular cleavage of the internucleotide linkage phosphorus. 
     In some embodiments, a 5′ hydroxyl group capping group is characterized in that it effectively caps the hydroxyl group such that it prevents the occurrence of “shortmers,” e.g., “n-m” (m and n are integers and m&lt;n; n is the number of bases in the targeted oligonucleotide) impurities that occur from the reaction of an oligonucleotide chain that fails to react in a first cycle but then reacts in one or more subsequent cycles. The presence of such shortmers, especially “n−1”, has a deleterious effect upon the purity of the crude oligonucleotide and makes final purification of the oligonucleotide tedious and generally low-yielding. 
     In some embodiments, a particular cap is selected based on its tendency to facilitate a particular type of reaction under particular conditions. For instance, in some embodiments, a capping group is selected for its ability to facilitate an E1 elimination reaction, which reaction cleaves the cap and/or auxiliary from the growing oligonucleotide. In some embodiments, a capping group is selected for its ability to facilitate an E2 elimination reaction, which reaction cleaves the cap and/or auxiliary from the growing oligonucleotide. In some embodiments, a capping group is selected for its ability to facilitate a β-elimination reaction, which reaction cleaves the cap and/or auxiliary from the growing oligonucleotide. 
     Modifying Step: 
     As used herein, the phrase “modifying step”, “modification step” and “P-modification step” are used interchangeably and refer generally to any one or more steps used to install a modified internucleotidic linkage. In some embodiments, the modified internucleotidic linkage having the structure of formula I. A P-modification step of the present invention occurs during assembly of a provided oligonucleotide rather than after assembly of a provided oligonucleotide is complete. Thus, each nucleotide unit of a provided oligonucleotide can be individually modified at the linkage phosphorus during the cycle within which the nucleotide unit is installed. 
     In some embodiments, a suitable P-modification reagent is a sulfur electrophile, selenium electrophile, oxygen electrophile, boronating reagent, or an azide reagent. 
     For instance, in some embodiments, a selemium reagent is elemental selenium, a selenium salt, or a substituted diselenide. In some embodiments, an oxygen electrophile is elemental oxygen, peroxide, or a substituted peroxide. In some embodiments, a boronating reagent is a borane-amine (e.g., N,N-diisopropylethylamine (BH 3 .DIPEA), borane-pyridine (BH 3 .Py), borane-2-chloropyridine (BH 3 .CPy), borane-aniline (BH 3 .An)), a borane-ether reagent (e.g., borane-tetrahydrofuran (BH 3 .THF)), a borane-dialkylsulfide reagent (e.g., BH 3 .Me 2 S), aniline-cyanoborane, or a triphenylphosphine-carboalkoxyborane. In some embodiments, an azide reagent is comprises an azide group capable of undergoing subsequent reduction to provide an amine group. 
     In some embodiments, a P-modification reagent is a sulfurization reagent as described herein. In some embodiments, a step of modifying comprises sulfurization of phosphorus to provide a phosphorothioate linkage or phosphorothioate triester linkage. In some embodiments, a step of modifying provides an oligonucleotide having an internucleotidic linkage of formula I. 
     In some embodiments, the present invention provides sulfurizing reagents, and methods of making, and use of the same. 
     In some embodiments, such sulfurizing reagents are thiosulfonate reagents. In some embodiments, a thiosulfonate reagent has a structure of formula S-I: 
                         
wherein:
 
R s1  is R; and
 
each of R, L and R 1  is independently as defined and described above and herein.
 
     In some embodiments, the sulfurizing reagent is a bis(thiosulfonate) reagent. In some embodiments, the bis(thiosulfonate) reagent has the structure of formula S-II: 
                         
wherein each of R s1  and L is independently as defined and described above and herein.
 
     As defined generally above, R s1  is R, wherein R is as defined and described above and herein. In some embodiments, R s1  is optionally substituted aliphatic, aryl, heterocyclyl or heteroaryl. In some embodiments, R s1  is optionally substituted alkyl. In some embodiments, R s1  is optionally substituted alkyl. In some embodiments, R s1  is methyl. In some embodiments, R s1  is cyanomethyl. In some embodiments, R s1  is nitromethyl. In some embodiments, R s1  is optionally substituted aryl. In some embodiments, R s1  is optionally substituted phenyl. In some embodiments, R s1  is phenyl. In some embodiments, R s1  is p-nitrophenyl. In some embodiments, R s1  is p-methylphenyl. In some embodiments, R s1  is p-chlorophenyl. In some embodiments, R s1  is o-chlorophenyl. In some embodiments, R s1  is 2,4,6-trichlorophenyl. In some embodiments, R s1  is pentafluorophenyl. In some embodiments, R s1  is optionally substituted heterocyclyl. In some embodiments, R s1  is optionally substituted heteroaryl. 
     In some embodiments, R s1 —S(O) 2 S— is 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
                         
In some embodiments, R s1 —S(O) 2 S— is
 
     
       
         
         
             
             
         
       
     
     In some embodiments, the sulfurizing reagent has the structure of S-I or S-II, wherein L is —S—R L3 — or —S—C(O)—R L3 —. In some embodiments, L is —S—R L3 — or —S—C(O)—R L3 —, wherein R L3  is an optionally substituted C 1 -C 6  alkylene. In some embodiments, L is —S—R L3 — or —S—C(O)—R L3 —, wherein R L3  is an optionally substituted C 1 -C 6  alkenylene. In some embodiments, L is —S—R L3 — or —S—C(O)—R L3 —, wherein R L3  is an optionally substituted C 1 -C 6  alkylene wherein one or more methylene units are optionally and independently replaced by an optionally substituted C 1 -C 6  alkenylene, arylene, or heteroarylene. In some embodiments, In some embodiments, R L3  is an optionally substituted —S—(C 1 -C 6  alkenylene)-, —S—(C 1 -C 6  alkylene)-, —S—(C 1 -C 6  alkylene)-arylene-(C 1 -C 6  alkylene)-, —S—CO-arylene-(C 1 -C 6  alkylene)-, or —S—CO—(C 1 -C 6  alkylene)-arylene-(C 1 -C 6  alkylene)-. In some embodiments, the sulfurizing reagent has the structure of S-I or S-II, wherein L is —S—R L3 — or —S—C(O)—R L3 —, and the sulfur atom is connected to R 1 . 
     In some embodiments, the sulfurizing reagent has the structure of S-I or S-II, wherein L is alkylene, alkenylene, arylene or heteroarylene. 
     In some embodiments, the sulfurizing reagent has the structure of S-I or S-II, wherein L is 
                         
In some embodiments, L is
 
                         
wherein the sulfur atom is connected to R 1 .
 
     In some embodiments, the sulfurizing reagent has the structure of S-I or S-II, wherein R 1  is 
                         
In some embodiments, R 1  is
 
                         
wherein the sulfur atom is connected to L.
 
     In some embodiments, the sulfurizing reagent has the structure of S-I or S-II, wherein L is 
                         
wherein the sulfur atom is connected to R 1 ; and R 1  is
 
                         
wherein the sulfur atom is connected to L.
 
     In some embodiments, the sulfurizing reagent has the structure of S-I or S-II, wherein R 1  is —S—R L2 , wherein R L2  is as defined and described above and herein. In some embodiments, R L2  is an optionally substituted group selected from —S—(C 1 -C 6  alkylene)-heterocyclyl, —S—(C 1 -C 6  alkenylene)-heterocyclyl, —S—(C 1 -C 6  alkylene)-N(R′) 2 , —S—(C 1 -C 6  alkylene)-N(R′) 3 , wherein each R′ is as defined above and described herein. 
     In some embodiments, -L-R 1  is —R L3 —S—R L2 , wherein each variable is independently as defined above and described herein. In some embodiments, -L-R 1  is —R L3 —C(O)—S—S—R L2 , wherein each variable is independently as defined above and described herein. 
     Exemplary bis(thiosulfonate) reagents of formula S-II are depicted below: 
     
       
         
         
             
             
         
       
     
     In some embodiments, the sulfurization reagent is a compound having one of the following formulae:
 
S 8 , R s2 —S—S—R s3 , or R s2 —S—X s —R s3 ,
 
wherein:
     each of R s2  and R s3  is independently an optionally substituted group selected from aliphatic, aminoalkyl, carbocyclyl, heterocyclyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, heteroaryloxy, acyl, amide, imide, or thiocarbonyl; or
       R s2  and R s3  are taken together with the atoms to which they are bound to form an optionally substituted heterocyclic or heteroaryl ring;   
       X s  is —S(O) 2 —, —O—, or —N(R′)—; and   R′ is as defined and described above and herein.   

     In some embodiments, the sulfurization reagent is S 8 , 
                         
In some embodiments, the sulfurization reagent is S 8 ,
 
                         
In some embodiments, the sulfurization reagent is
 
     
       
         
         
             
             
         
       
     
     Exemplary sulfuring reagents are depicted in Table 5 below. 
     
       
         
           
               
             
               
                 TABLE 5 
               
               
                   
               
               
                 Exemplary sulfurization reagents. 
               
               
                   
               
             
            
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 S 8   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     In some embodiments, a provided sulfurization reagent is used to modify an H-phosphonate. For instance, in some embodiments, an H-phosphonate oligonucleotide is synthesized using, e.g., a method of Wada I or Wada II, and is modified using a sulfurization reagent of formula S-I or S-II: 
                         
wherein each of R S1 , L, and R 1  are as described and defined above and herein.
 
     In some embodiments, the present invention provides a process for synthesizing a phosphorothioate triester, comprising steps of: 
     i) reacting an H-phosphonate of structure: 
     
       
         
         
             
             
         
       
     
     wherein each of W, Y, and Z are as described and defined above and herein, with a silylating reagent to provide a silyloxyphosphonate; and 
     ii) reacting the silyloxyphosphonate with a sulfurization reagent of structure S-I or S-II: 
     
       
         
         
             
             
         
       
     
     to provide a phosphorothiotriester. 
     In some embodiments, a selenium electrophile is used instead of a sulfurizing reagent to introduce modification to the internucleotidic linkage. In some embodiments, a selenium electrophile is a compound having one of the following formulae:
 
Se, R s2 —Se—Se—R s3 , or R s2 —Se—X s —R s3 ,
 
wherein:
     each of R s2  and R s3  is independently an optionally substituted group selected from aliphatic, aminoalkyl, carbocyclyl, heterocyclyl, heterocycloalkyl, aryl, heteroaryl, alkyloxy, aryloxy, heteroaryloxy, acyl, amide, imide, or thiocarbonyl; or
       R s2  and R s3  are taken together with the atoms to which they are bound to form an optionally substituted heterocyclic or heteroaryl ring;   
       X s  is —S(O) 2 —, —O—, or —N(R′)—; and   R′ is as defined and described above and herein.   

     In other embodiments, the selenium electrophile is a compound of Se, KSeCN, 
                         
In some embodiments, the selenium electrophile is Se or
 
     
       
         
         
             
             
         
       
     
     In some embodiments, a sulfurization reagent for use in accordance with the present invention is characterized in that the moiety transferred to phosphorus during sulfurization is a substituted sulfur (e.g., —SR) as opposed to a single sulfur atom (e.g., —S −  or ═S). 
     In some embodiments, a sulfurization reagent for use in accordance with the present invention is characterized in that the activity of the reagent is tunable by modifying the reagent with a certain electron withdrawing or donating group. 
     In some embodiments, a sulfurization reagent for use in accordance with the present invention is characterized in that it is crystalline. In some embodiments, a sulfurization reagent for use in accordance with the present invention is characterized in that it has a high degree of crystallinity. In certain embodiments, a sulfurization reagent for use in accordance with the present invention is characterized by ease of purification of the reagent via, e.g., recrystallization. In certain embodiments, a sulfurization reagent for use in accordance with the present invention is characterized in that it is substantially free from sulfur-containing impurities. In some embodiments, sulfurization reagents which are substantially free from sulfur-containing impurities show increased efficiency. 
     In some embodiments, the provided chirally controlled oligonucleotide comprises one or more phosphate diester linkages. To synthesize such chirally controlled oligonucleotides, one or more modifying steps are optionally replaced with an oxidation step to install the corresponding phosphate diester linkages. In some embodiments, the oxidation step is performed in a fashion similar to ordinary oligonucleotide synthesis. In some embodiments, an oxidation step comprises the use of I 2 . In some embodiments, an oxidation step comprises the use of I 2  and pyridine. In some embodiments, an oxidation step comprises the use of 0.02 M I 2  in a THF/pyridine/water (70:20:10—v/v/v) co-solvent system. An exemplary cycle is depicted in Scheme I-c. 
     In some embodiments, a phosphorothioate precursor is used to synthesize chirally controlled oligonucleotides comprising phosphorothioate linkages. In some embodiments, such a phosphorothioate precursor is 
                         
In some embodiments,
 
                         
is converted into phosphorothioate diester linkages during standard deprotection/release procedure after cycle exit. Examples are further depicted below.
 
     In some embodiments, the provided chirally controlled oligonucleotide comprises one or more phosphate diester linkages and one or more phosphorothioate diester linkages. In some embodiments, the provided chirally controlled oligonucleotide comprises one or more phosphate diester linkages and one or more phosphorothioate diester linkages, wherein at least one phosphate diester linkage is installed after all the phosphorothioate diester linkages when synthesized from 3′ to 5′. To synthesize such chirally controlled oligonucleotides, in some embodiments, one or more modifying steps are optionally replaced with an oxidation step to install the corresponding phosphate diester linkages, and a phosphorothioate precursor is installed for each of the phosphorothioate diester linkages. In some embodiments, a phosphorothioate precursor is converted to a phosphorothioate diester linkage after the desired oligonucleotide length is achieved. In some embodiments, the deprotection/release step during or after cycle exit converts the phosphorothioate precursors into phosphorothioate diester linkages. In some embodiments, a phosphorothioate precursor is characterized in that it has the ability to be removed by a beta-elimination pathway. In some embodiments, a phosphorothioate precursor is 
                         
As understood by one of ordinary skill in the art, one of the benefits of using a phosphorothioate precursor, for instance,
 
                         
during synthesis is that
 
                         
is more stable than phosphorothioate in certain conditions.
 
     In some embodiments, a phosphorothioate precursor is a phosphorus protecting group as described herein, e.g., 2-cyanoethyl (CE or Cne), 2-trimethylsilylethyl, 2-nitroethyl, 2-sulfonylethyl, methyl, benzyl, o-nitrobenzyl, 2-(p-nitrophenyl)ethyl (NPE or Npe), 2-phenylethyl, 3-(N-tert-butylcarboxamido)-1-propyl, 4-oxopentyl, 4-methylthio-1-butyl, 2-cyano-1,1-dimethylethyl, 4-N-methylaminobutyl, 3-(2-pyridyl)-1-propyl, 2-[N-methyl-N-(2-pyridyl)]aminoethyl, 2-(N-formyl,N-methyl)aminoethyl, 4-[N-methyl-N-(2,2,2-trifluoroacetyl)amino]butyl. Examples are further depicted below. 
     Methods for synthesizing a desired sulfurization reagent are described herein and in the examples section. 
     As noted above, in some embodiments, sulfurization occurs under conditions which cleave the chiral reagent from the growing oligonucleotide. In some embodiments, sulfurization occurs under conditions which do not cleave the chiral reagent from the growing oligonucleotide. 
     In some embodiments, a sulfurization reagent is dissolved in a suitable solvent and delivered to the column. In certain embodiments, the solvent is a polar aprotic solvent such as a nitrile solvent. In some embodiments, the solvent is acetonitrile. In some embodiments, a solution of sulfurization reagent is prepared by mixing a sulfurization reagent (e.g., a thiosulfonate derivative as described herein) with BSTFA (N,O-bis-trimethylsilyl-trifluoroacetamide) in a nitrile solvent (e.g., acetonitrile). In some embodiments, BSTFA is not included. For example, the present inventors have found that relatively more reactive sulfurization reagents of general formula R s2 —S—S(O) 2 —R s3  can often successfully participate in sulfurization reactions in the absence of BSTFA. To give but one example, the inventors have demonstrated that where R s2  is p-nitrophenyl and R s3  is methyl then no BSTFA is required. In light of this disclosure, those skilled in the art will readily be able to determine other situations and/or sulfurization reagents that do not require BSTFA. 
     In some embodiments, the sulfurization step is performed at room temperature. In some embodiments, the sulfurization step is performed at lower temperatures such as about 0° C., about 5° C., about 10° C., or about 15° C. In some embodiments, the sulfurization step is performed at elevated temperatures of greater than about 20° C. 
     In some embodiments, a sulfurization reaction is run for about 1 minute to about 120 minutes. In some embodiments, a sulfurization reaction is run for about 1 minute to about 90 minutes. In some embodiments, a sulfurization reaction is run for about 1 minute to about 60 minutes. In some embodiments, a sulfurization reaction is run for about 1 minute to about 30 minutes. In some embodiments, a sulfurization reaction is run for about 1 minute to about 25 minutes. In some embodiments, a sulfurization reaction is run for about 1 minute to about 20 minutes. In some embodiments, a sulfurization reaction is run for about 1 minute to about 15 minutes. In some embodiments, a sulfurization reaction is run for about 1 minute to about 10 minutes. In some embodiments, a sulfurization reaction is run for about 5 minute to about 60 minutes. 
     In some embodiments, a sulfurization reaction is run for about 5 minutes. In some embodiments, a sulfurization reaction is run for about 10 minutes. In some embodiments, a sulfurization reaction is run for about 15 minutes. In some embodiments, a sulfurization reaction is run for about 20 minutes. In some embodiments, a sulfurization reaction is run for about 25 minutes. In some embodiments, a sulfurization reaction is run for about 30 minutes. In some embodiments, a sulfurization reaction is run for about 35 minutes. In some embodiments, a sulfurization reaction is run for about 40 minutes. In some embodiments, a sulfurization reaction is run for about 45 minutes. In some embodiments, a sulfurization reaction is run for about 50 minutes. In some embodiments, a sulfurization reaction is run for about 55 minutes. In some embodiments, a sulfurization reaction is run for about 60 minutes. 
     It was unexpectedly found that certain of the sulfurization modification products made in accordance with methods of the present invention are unexpectedly stable. In some embodiments, it the unexpectedly stable products are phosphorothioate triesters. In some embodiments, the unexpectedly stable products are chirally controlled oligonucleotides comprising one or more internucleotidic linkages having the structure of formula I-c. 
     One of skill in the relevant arts will recognize that sulfurization methods described herein and sulfurization reagents described herein are also useful in the context of modifying H-phosphonate oligonucleotides such as those described in Wada II (WO2010/064146). 
     In some embodiments, the sulfurization reaction has a stepwise sulfurization efficiency that is at least about 80%, 85%, 90%, 95%, 96%, 97%, or 98%. In some embodiments, the sulfurization reaction provides a crude dinucleotide product composition that is at least 98% pure. In some embodiments, the sulfurization reaction provides a crude tetranucleotide product composition that is at least 90% pure. In some embodiments, the sulfurization reaction provides a crude dodecanucleotide product composition that is at least 70% pure. In some embodiments, the sulfurization reaction provides a crude icosanucleotide product composition that is at least 50% pure. 
     Once the step of modifying the linkage phosphorus is complete, the oligonucleotide undergoes another deblock step in preparation for re-entering the cycle. In some embodiments, a chiral auxiliary remains intact after sulfurization and is deblocked during the subsequent deblock step, which necessarily occurs prior to re-entering the cycle. The process of deblocking, coupling, capping, and modifying, are repeated until the growing oligonucleotide reaches a desired length, at which point the oligonucleotide can either be immediately cleaved from the solid support or left attached to the support for purification purposes and later cleaved. In some embodiments, one or more protecting groups are present on one or more of the nucleotide bases, and cleavage of the oligonucleotide from the support and deprotection of the bases occurs in a single step. In some embodiments, one or more protecting groups are present on one or more of the nucleotide bases, and cleavage of the oligonucleotide from the support and deprotection of the bases occurs in more than one steps. In some embodiments, deprotection and cleavage from the support occurs under basic conditions using, e.g., one or more amine bases. In certain embodiments, the one or more amine bases comprise propyl amine. In certain embodiments, the one or more amine bases comprise pyridine. 
     In some embodiments, cleavage from the support and/or deprotection occurs at elevated temperatures of about 30° C. to about 90° C. In some embodiments, cleavage from the support and/or deprotection occurs at elevated temperatures of about 40° C. to about 80° C. In some embodiments, cleavage from the support and/or deprotection occurs at elevated temperatures of about 50° C. to about 70° C. In some embodiments, cleavage from the support and/or deprotection occurs at elevated temperatures of about 60° C. In some embodiments, cleavage from the support and/or deprotection occurs at ambient temperatures. 
     Exemplary purification procedures are described herein and/or are known generally in the relevant arts. 
     Noteworthy is that the removal of the chiral auxiliary from the growing oligonucleotide during each cycle is beneficial for at least the reasons that (1) the auxiliary will not have to be removed in a separate step at the end of the oligonucleotide synthesis when potentially sensitive functional groups are installed on phosphorus; and (2) unstable phosphorus-auxiliary intermediates prone to undergoing side reactions and/or interfering with subsequent chemistry are avoided. Thus, removal of the chiral auxiliary during each cycle makes the overall synthesis more efficient. 
     While the step of deblocking in the context of the cycle is described above, additional general methods are included below. 
     Deblocking Step 
     In some embodiments, the step of coupling is preceded by a step of deblocking. For instance, in some embodiments, the 5′ hydroxyl group of the growing oligonucleotide is blocked (i.e., protected) and must be deblocked in order to subsequently react with a nucleoside coupling partner. 
     In some embodiments, acidification is used to remove a blocking group. In some embodiments, the acid is a Brønsted acid or Lewis acid. Useful Brønsted acids are carboxylic acids, alkylsulfonic acids, arylsulfonic acids, phosphoric acid and its derivatives, phosphonic acid and its derivatives, alkylphosphonic acids and their derivatives, arylphosphonic acids and their derivatives, phosphinic acid, dialkylphosphinic acids, and diarylphosphinic acids which have a pKa (25° C. in water) value of −0.6 (trifluoroacetic acid) to 4.76 (acetic acid) in an organic solvent or water (in the case of 80% acetic acid). The concentration of the acid (1 to 80%) used in the acidification step depends on the acidity of the acid. Consideration to the acid strength must be taken into account as strong acid conditions will result in depurination/depyrimidination, wherein purinyl or pyrimidinyl bases are cleaved from ribose ring and or other sugar ring. In some embodiments, an acid is selected from R a1 COOH, R a1 SO 3 H, R a3 SO 3 H, 
                         
wherein each of R a1  and R a2  is independently hydrogen or an optionally substituted alkyl or aryl, and R a3  is an optionally substituted alkyl or aryl.
 
     In some embodiments, acidification is accomplished by a Lewis acid in an organic solvent. Exemplary such useful Lewis acids are Zn(X a ) 2  wherein X a  is Cl, Br, I, or CF 3 SO 3 . 
     In some embodiments, the step of acidifying comprises adding an amount of a Brønsted or Lewis acid effective to remove a blocking group without removing purine moieties from the condensed intermediate. 
     Acids that are useful in the acidifying step also include, but are not limited to 10% phosphoric acid in an organic solvent, 10% hydrochloric acid in an organic solvent, 1% trifluoroacetic acid in an organic solvent, 3% dichloroacetic acid or trichloroacetic acid in an organic solvent or 80% acetic acid in water. The concentration of any Brønsted or Lewis acid used in this step is selected such that the concentration of the acid does not exceed a concentration that causes cleavage of a nucleobase from a sugar moiety. 
     In some embodiments, acidification comprises adding 1% trifluoroacetic acid in an organic solvent. In some embodiments, acidification comprises adding about 0.1% to about 8% trifluoroacetic acid in an organic solvent. In some embodiments, acidification comprises adding 3% dichloroacetic acid or trichloroacetic acid in an organic solvent. In some embodiments, acidification comprises adding about 0.1% to about 10% dichloroacetic acid or trichloroacetic acid in an organic solvent. In some embodiments, acidification comprises adding 3% trichloroacetic acid in an organic solvent. In some embodiments, acidification comprises adding about 0.1% to about 10% trichloroacetic acid in an organic solvent. In some embodiments, acidification comprises adding 80% acetic acid in water. In some embodiments, acidification comprises adding about 50% to about 90%, or about 50% to about 80%, about 50% to about 70%, about 50% to about 60%, about 70% to about 90% acetic acid in water. In some embodiments, the acidification comprises the further addition of cation scavengers to an acidic solvent. In certain embodiments, the cation scavengers can be triethylsilane or triisopropylsilane. In some embodiments, a blocking group is deblocked by acidification, which comprises adding 1% trifluoroacetic acid in an organic solvent. In some embodiments, a blocking group is deblocked by acidification, which comprises adding 3% dichloroacetic acid in an organic solvent. In some embodiments, a blocking group is deblocked by acidification, which comprises adding 3% trichloroacetic acid in an organic solvent. In some embodiments, a blocking group is deblocked by acidification, which comprises adding 3% trichloroacetic acid in dichloromethane. 
     In certain embodiments, methods of the present invention are completed on a synthesizer and the step of deblocking the hydroxyl group of the growing oligonucleotide comprises delivering an amount solvent to the synthesizer column, which column contains a solid support to which the oligonucleotide is attached. In some embodiments, the solvent is a halogenated solvent (e.g., dichloromethane). In certain embodiments, the solvent comprises an amount of an acid. In some embodiments, the solvent comprises an amount of an organic acid such as, for instance, trichloroacetic acid. In certain embodiments, the acid is present in an amount of about 1% to about 20% w/v. In certain embodiments, the acid is present in an amount of about 1% to about 10% w/v. In certain embodiments, the acid is present in an amount of about 1% to about 5% w/v. In certain embodiments, the acid is present in an amount of about 1 to about 3% w/v. In certain embodiments, the acid is present in an amount of about 3% w/v. Methods for deblocking a hydroxyl group are described further herein. In some embodiments, the acid is present in 3% w/v is dichloromethane. 
     In some embodiments, the chiral auxiliary is removed before the deblocking step. In some embodiments, the chiral auxiliary is removed during the deblocking step. 
     In some embodiments, cycle exit is performed before the deblocking step. In some embodiments, cycle exit is preformed after the deblocking step. 
     General Conditions for Blocking Group/Protecting Group Removal 
     Functional groups such as hydroxyl or amino moieties which are located on nucleobases or sugar moieties are routinely blocked with blocking (protecting) groups (moieties) during synthesis and subsequently deblocked. In general, a blocking group renders a chemical functionality of a molecule inert to specific reaction conditions and can later be removed from such functionality in a molecule without substantially damaging the remainder of the molecule (see e.g., Green and Wuts, Protective Groups in Organic Synthesis, 2nd Ed., John Wiley &amp; Sons, New York, 1991). For example, amino groups can be blocked with nitrogen blocking groups such as phthalimido, 9-fludrenylmethoxycarbonyl (FMOC), triphenylmethylsulfenyl, t-BOC, 4,4′-dimethoxytrityl (DMTr), 4-methoxytrityl (MMTr), 9-phenylxanthin-9-yl (Pixyl), trityl (Tr), or 9-(p-methoxyphenyl)xanthin-9-yl (MOX). Carboxyl groups can be protected as acetyl groups. Hydroxy groups can be protected such as tetrahydropyranyl (THP), t-butyldimethylsilyl (TBDMS), 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp), 1-(2-fluorophenyl)-4-methoxypiperidin-4-yl (Fpmp), 1-(2-chloroethoxy)ethyl, 3-methoxy-1,5-dicarbomethoxypentan-3-yl (MDP), bis(2-acetoxyethoxy)methyl (ACE), triisopropylsilyloxymethyl (TOM), 1-(2-cyanoethoxy)ethyl (CEE), 2-cyanoethoxymethyl (CEM), [4-(N-dichloroacetyl-N-methylamino)benzyloxy]methyl, 2-cyanoethyl (CN), pivaloyloxymethyl (PivOM), levunyloxymethyl (ALE). Other representative hydroxyl blocking groups have been described (see e.g., Beaucage et al.,  Tetrahedron,  1992, 46, 2223). In some embodiments, hydroxyl blocking groups are acid-labile groups, such as the trityl, monomethoxytrityl, dimethoxytrityl, trimethoxytrityl, 9-phenylxanthin-9-yl (Pixyl) and 9-(p-methoxyphenyl)xanthin-9-yl (MOX). Chemical functional groups can also be blocked by including them in a precursor form. Thus an azido group can be considered a blocked form of an amine as the azido group is easily converted to the amine. Further representative protecting groups utilized in nucleic acid synthesis are known (see e.g. Agrawal et al., Protocols for Oligonucleotide Conjugates, Eds., Humana Press, New Jersey, 1994, Vol. 26, pp. 1-72). 
     Various methods are known and used for removal of blocking groups from nucleic acids. In some embodiments, all blocking groups are removed. In some embodiments, a portion of blocking groups are removed. In some embodiments, reaction conditions can be adjusted to selectively remove certain blocking groups. 
     In some embodiments, nucleobase blocking groups, if present, are cleavable with an acidic reagent after the assembly of a provided oligonucleotide. In some embodiment, nucleobase blocking groups, if present, are cleavable under neither acidic nor basic conditions, e.g. cleavable with fluoride salts or hydrofluoric acid complexes. In some embodiments, nucleobase blocking groups, if present, are cleavable in the presence of base or a basic solvent after the assembly of a provided oligonucleotide. In certain embodiments, one or more of the nucleobase blocking groups are characterized in that they are cleavable in the presence of base or a basic solvent after the assembly of a provided oligonucleotide but are stable to the particular conditions of one or more earlier deprotection steps occurring during the assembly of the provided oligonucleotide. 
     In some embodiments, blocking groups for nucleobases are not required. In some embodiments, blocking groups for nucleobases are required. In some embodiments, certain nucleobases require one or more blocking groups while other nucleobases do not require one or more blocking groups. 
     In some embodiments, the oligonucleotide is cleaved from the solid support after synthesis. In some embodiments, cleavage from the solid support comprises the use of propylamine. In some embodiments, cleavage from the solid support comprises the use of propylamine in pyridine. In some embodiments, cleavage from the solid support comprises the use of 20% propylamine in pyridine. In some embodiments, cleavage from the solid support comprises the use of propylamine in anhydrous pyridine. In some embodiments, cleavage from the solid support comprises the use of 20% propylamine in anhydrous pyridine. In some embodiments, cleavage from the solid support comprises use of a polar aprotic solvent such as acetonitrile, NMP, DMSO, sulfone, and/or lutidine. In some embodiments, cleavage from the solid support comprises use of solvent, e.g., a polar aprotic solvent, and one or more primary amines (e.g., a C 1-10  amine), and/or one or more of methoxylamine, hydrazine, and pure anhydrous ammonia. 
     In some embodiments, deprotection of oligonucleotide comprises the use of propylamine. In some embodiments, deprotection of oligonucleotide comprises the use of propylamine in pyridine. In some embodiments, deprotection of oligonucleotide comprises the use of 20% propylamine in pyridine. In some embodiments deprotection of oligonucleotide comprises the use of propylamine in anhydrous pyridine. In some embodiments, deprotection of oligonucleotide comprises the use of 20% propylamine in anhydrous pyridine. 
     In some embodiments, the oligonucleotide is deprotected during cleavage. 
     In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at about room temperature. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at elevated temperature. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at above about 30° C., 40° C., 50° C., 60° C., 70° C., 80° C. 90° C. or 100° C. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at about 30° C., 40° C., 50° C., 60° C., 70° C., 80° C. 90° C. or 100° C. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at about 40-80° C. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at about 50-70° C. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at about 60° C. 
     In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for more than 0.1 hr, 1 hr, 2 hrs, 5 hrs, 10 hrs, 15 hrs, 20 hrs, 24 hrs, 30 hrs, or 40 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 0.1-5 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 3-10 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 5-15 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 10-20 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 15-25 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 20-40 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 2 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 5 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 10 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 15 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 18 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed for about 24 hrs. 
     In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at room temperature for more than 0.1 hr, 1 hr, 2 hrs, 5 hrs, 10 hrs, 15 hrs, 20 hrs, 24 hrs, 30 hrs, or 40 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at room temperature for about 5-48 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at room temperature for about 10-24 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at room temperature for about 18 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at elevated temperature for more than 0.1 hr, 1 hr, 2 hrs, 5 hrs, 10 hrs, 15 hrs, 20 hrs, 24 hrs, 30 hrs, or 40 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at elevated temperature for about 0.5-5 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at about 60° C. for about 0.5-5 hrs. In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide, is performed at about 60° C. for about 2 hrs. 
     In some embodiments, cleavage of oligonucleotide from solid support, or deprotection of oligonucleotide comprises the use of propylamine and is performed at room temperature or elevated temperature for more than 0.1 hr, 1 hr, 2 hrs, 5 hrs, 10 hrs, 15 hrs, 20 hrs, 24 hrs, 30 hrs, or 40 hrs. Exemplary conditions are 20% propylamine in pyridine at room temperature for about 18 hrs, and 20% propylamine in pyridine at 60° C. for about 18 hrs, 
     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved; 
     wherein the chirally controlled oligonucleotide comprises at least one phosphorothioate diester linkage or at least one internucleotidic linkage having the structure of formula I-c. 
     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved; 
     wherein: 
     at least one cycle of (1) to (4) forms an phosphorothioate diester linkage. 
     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved; 
     wherein: 
     at least one cycle of (1) to (4) forms an internucleotidic linkage having the structure of formula I-c. 
     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved; 
     wherein: 
     
         
         the coupling step comprises the use of an activating group and 
       
    
                         
wherein B PRO  is a protected nucleobase;
     the capping step comprising capping of the amino group in the chiral auxiliary and the capping of unreacted 5′-OH;   the modifying step comprising installation of —S-L-R 1  group to the linkage phosphorus, wherein each of L and R 1  is independently as defined above and described herein;   the delocking step comprising the use of an acid.   

     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved; 
     wherein: 
     
         
         the coupling step comprises the use of CMPT and 
       
    
                         
wherein B PRO  is a protected nucleobase;
     the capping step comprising capping of the amino group in the chiral auxiliary and the capping of unreacted 5′-OH;   the modifying step comprising installation of —S-L-R 1  group to the linkage phosphorus, wherein each of L and R 1  is independently as defined above and described herein;   the deblocking step comprising the use of an acid.   

     In some embodiments, an activator is a “Wada” activator, i.e., the activator is from any one of Wada I, II, or III documents cited above. 
     Exemplary activating groups are depicted below: 
     
       
         
         
             
             
         
       
     
     An exemplary cycle is depicted in Scheme I-b. 
     
       
         
         
             
             
         
       
     
     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved; 
     wherein: 
     the chirally controlled oligonucleotide comprises at least one phosphorothioate diester linkage or at least one internucleotidic linkage of formula I-c, and at least one phosphate diester internucleotidic linkage; and 
     at least one modifying step is replaced by an oxidization step. 
     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved; 
     wherein: 
     the chirally controlled oligonucleotide comprises at least one phosphorothioate diester linkage or at least one internucleotidic linkage of formula I-c, and at least one phosphate diester internucleotidic linkage; and 
     at least one modifying step is replaced by an oxidization step comprising the use of I 2 . 
     An exemplary cycle is illustrated in Scheme I-c. 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     In Scheme I-c, oligonucleotide (or nucleotide, or oligonucleotide with modified internucleotidic linkage) on solid support (C-1) is coupled with phosphoramidite C-2. After coupling and capping, an oxidation step is performed. After deblocking, a phosphate diester linkage is formed. The cycle product C-3 can either re-enter cycle C to install more phosphate diester linkage, or enter other cycles to install other types of internucleotidic linkages, or go to cycle exit. 
     In some embodiments, non-chirally pure phosphoramidite can be used instead of C-2 in Scheme I-c. In some embodiments, β-cyanoethylphosphoramidites protected with DMTr is used. In some embodiments, the phosphoramidite being used has the structure of 
     
       
         
         
             
             
         
       
     
     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved; 
     wherein: 
     the chirally controlled oligonucleotide comprises one or more phosphorothioate diester linkages; and 
     one or more phosphorothioate diester precursors are formed for each of the corresponding phosphorothioate diester linkage. 
     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved; 
     wherein: 
     the chirally controlled oligonucleotide comprises at least one phosphorothioate diester linkages; one or more phosphorothioate diester precursors are formed for each of the corresponding phosphorothioate diester linkage; and 
     each phosphorothioate diester precursor is converted to a phosphorothioate diester linkage after the desired length is achieved. 
     In some embodiments, the present invention provides methods for making a chirally controlled oligonucleotide comprising steps of: 
     (1) coupling; 
     (2) capping; 
     (3) modifying; 
     (4) deblocking; and 
     (5) repeating steps (1)-(4) until a desired length is achieved; 
     wherein: 
     the chirally controlled oligonucleotide comprises at least one phosphorothioate diester linkages and at least one phosphate diester internucleotidic linkage; 
     at least one modifying step is replaced by an oxidization step; and 
     at least one modifying step is performed to install a phosphorothioate diester precursor for each of the phosphorothioate diester linkages; and 
     each phosphorothioate diester precursor is converted to a phosphorothioate diester linkage after the desired length is achieved. 
     In some embodiments, the use of a phosphorothioate diester precursor increases the stability of oligonucleotide during synthesis. In some embodiments, the use of a phosphorothioate diester precursor improves the efficiency of chirally controlled oligonucleotide synthesis. In some embodiments, the use of a phosphorothioate diester precursor improves the yield of chirally controlled oligonucleotide synthesis. In some embodiments, the use of a phosphorothioate diester precursor improves the product purity of chirally controlled oligonucleotide synthesis. 
     In some embodiments, the phosphorothioate diester precursor in the above-mentioned methods is 
                         
In some embodiments,
 
                         
is converted to a phosphorothioate diester linkage during deprotection/release. An exemplary cycle is depicted in Scheme I-d. More examples are depicted below.
 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     As illustrated in Scheme I-d, both phosphorothioate and phosphate diester linkages can be incorporated into the same chirally controlled oligonucleotide. As understood by a person of ordinary skill in the art, the provided methods do not require that the phosphorothioate diester and the phosphate diester to be consecutive—other internucleotidic linkages can form between them using a cycle as described above. In Scheme I-d, phosphorothioate diester precursors, 
                         
are installed in place of the phosphorothioate diester linkages. In some embodiments, such replacement provided increased synthesis efficiency during certain steps, for instance, the oxidation step. In some embodiments, the use of phosphorothioate diester precursors generally improve the stability of chirally controlled oligonucleotides during synthesis and/or storage. After cycle exit, during deprotection/release, the phosphorothioate diester precursor is converted to phosphorothioate diester linkage. In some embodiments, it is beneficial to use phosphorothioate diester precursor even when no phosphate diester linkage is present in the chirally controlled oligonucleotide, or no oxidation step is required during synthesis.
 
     As in Scheme I-c, in some embodiments, non-chirally pure phosphoramidite can be used for cycles comprising oxidation steps. In some embodiments, β-cyanoethylphosphoramidites protected with DMTr is used. In some embodiments, the phosphoramidite being used has the structure of 
     
       
         
         
             
             
         
       
     
     In some embodiments, methods of the present invention provide chirally controlled oligonucleotide compositions that are enriched in a particular oligonucleotide type. 
     In some embodiments, at least about 10% of a provided crude composition is of a particular oligonucleotide type. In some embodiments, at least about 20% of a provided crude composition is of a particular oligonucleotide type. In some embodiments, at least about 30% of a provided crude composition is of a particular oligonucleotide type. In some embodiments, at least about 40% of a provided crude composition is of a particular oligonucleotide type. In some embodiments, at least about 50% of a provided crude composition is of a particular oligonucleotide type. In some embodiments, at least about 60% of a provided crude composition is of a particular oligonucleotide type. In some embodiments, at least about 70% of a provided crude composition is of a particular oligonucleotide type. In some embodiments, at least about 80% of a provided crude composition is of a particular oligonucleotide type. In some embodiments, at least about 90% of a provided crude composition is of a particular oligonucleotide type. In some embodiments, at least about 95% of a provided crude composition is of a particular oligonucleotide type. 
     In some embodiments, at least about 1% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 2% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 3% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 4% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 5% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 10% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 20% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 30% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 40% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 50% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 60% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 70% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 80% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 90% of a provided composition is of a particular oligonucleotide type. In some embodiments, at least about 95% of a provided composition is of a particular oligonucleotide type. 
     Biological Applications 
     As discussed in detail herein, the present invention provides, among other things, a chirally controlled oligonucleotide composition, meaning that the composition contains a plurality of oligonucleotides of at least one type. Each oligonucleotide molecule of a particular “type” is comprised of preselected (e.g., predetermined) structural elements with respect to: (1) base sequence; (2) pattern of backbone linkages; (3) pattern of backbone chiral centers; and (4) pattern of backbone P-modification moieties. In some embodiments, provided oligonucleotide compositions contain oligonucleotides that are prepared in a single synthesis process. In some embodiments, provided compositions contain oligonucleotides having more than one chiral configuration within a single oligonucleotide molecule (e.g., where different residues along the oligonucleotide have different stereochemistry); in some such embodiments, such oligonucleotides may be obtained in a single synthesis process, without the need for secondary conjugation steps to generate individual oligonucleotide molecules with more than one chiral configuration. 
     Oligonucleotide compositions as provided herein can be used as agents for modulating a number of cellular processes and machineries, including but not limited to, transcription, translation, immune responses, epigenetics, etc. In addition, oligonucleotide compositions as provided herein can be used as reagents for research and/or diagnostic purposes. One of ordinary skill in the art will readily recognize that the present invention disclosure herein is not limited to particular use but is applicable to any situations where the use of synthetic oligonucleitides is desirable. Among other things, provided compositions are useful in a variety of therapeutic, diagnostic, agricultural, and/or research applications. 
     In some embodiments, provided oligonucleotide compositions comprise oligonucleotides and/or residues thereof that include one or more structural modifications as described in detail herein. In some embodiments, provided oligonucleotide compositions comprise oligonucleoties that contain one or more nucleic acid analogs. In some embodiments, provided oligonucleotide compositions comprise oligonucleotides that contain one or more artificial nucleic acids or residues, including but not limited to: peptide nucleic acids (PNA), Morpholino and locked nucleic acids (LNA), glycon nucleic acids (GNA), threose nucleic acids (TNA), Xeno nucleic acids (ZNA), and any combination thereof. 
     In any of the embodiments, the invention is useful for oligonucleotide-based modulation of gene expression, immune response, etc. Accordingly, stereo-defined, oligonucleotide compositions of the invention, which contain oligonucleotides of predetermined type (i.e., which are chirally controlled, and optionally chirally pure), can be used in lieu of conventional stereo-random or chirally impure counterparts. In some embodiments, provided compositions show enhanced intended effects and/or reduced unwanted side effects. Certain embodiments of biological and clinical/therapeutic applications of the invention are discussed explicitly below. 
     Various dosing regimens can be utilized to administer provided chirally controlled oligonucleotide compositions. In some embodiments, multiple unit doses are administered, separated by periods of time. In some embodiments, a given composition has a recommended dosing regimen, which may involve one or more doses. In some embodiments, a dosing regimen comprises a plurality of doses each of which are separated from one another by a time period of the same length; in some embodiments, a dosing regimen comprises a plurality of doses and at least two different time periods separating individual doses. In some embodiments, all doses within a dosing regimen are of the same unit dose amount. In some embodiments, different doses within a dosing regimen are of different amounts. In some embodiments, a dosing regimen comprises a first dose in a first dose amount, followed by one or more additional doses in a second dose amount different from the first dose amount. In some embodiments, a dosing regimen comprises a first dose in a first dose amount, followed by one or more additional doses in a second (or subsequent) dose amount that is same as or different from the first dose (or another prior dose) amount. In some embodiments, a dosing regimen comprises administering at least one unit dose for at least one day. In some embodiments, a dosing regimen comprises administering more than one dose over a time period of at least one day, and sometimes more than one day. In some embodiments, a dosing regimen comprises administering multiple doses over a time period of at least week. In some embodiments, the time period is at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40 or more (e.g., about 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100 or more) weeks. In some embodiments, a dosing regimen comprises administering one dose per week for more than one week. In some embodiments, a dosing regimen comprises administering one dose per week for 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40 or more (e.g., about 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100 or more) weeks. In some embodiments, a dosing regimen comprises administering one dose every two weeks for more than two week period. In some embodiments, a dosing regimen comprises administering one dose every two weeks over a time period of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40 or more (e.g., about 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100 or more) weeks. In some embodiments, a dosing regimen comprises administering one dose per month for one month. In some embodiments, a dosing regimen comprises administering one dose per month for more than one month. In some embodiments, a dosing regimen comprises administering one dose per month for 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or more months. In some embodiments, a dosing regimen comprises administering one dose per week for about 10 weeks. In some embodiments, a dosing regimen comprises administering one dose per week for about 20 weeks. In some embodiments, a dosing regimen comprises administering one dose per week for about 30 weeks. In some embodiments, a dosing regimen comprises administering one dose per week for 26 weeks. In some embodiments, a chirally controlled oligonucleotide composition is administered according to a dosing regimen that differs from that utilized for a chirally uncontrolled (e.g., stereorandom) oligonucleotide composition of the same sequence, and/or of a different chirally controlled oligonucleotide composition of the same sequence. In some embodiments, a chirally controlled oligonucleotide composition is administered according to a dosing regimen that is reduced as compared with that of a chirally uncontrolled (e.g., stereorandom) oligonucleotide composition of the same sequence in that it achieves a lower level of total exposure over a given unit of time, involves one or more lower unit doses, and/or includes a smaller number of doses over a given unit of time. In some embodiments, a chirally controlled oligonucleotide composition is administered according to a dosing regimen that extends for a longer period of time than does that of a chirally uncontrolled (e.g., stereorandom) oligonucleotide composition of the same sequence Without wishing to be limited by theory, Applicant notes that in some embodiments, the shorter dosing regimen, and/or longer time periods between doses, may be due to the improved stability, bioavailability, and/or efficacy of a chirally controlled oligonucleotide composition. In some embodiments, a chirally controlled oligonucleotide composition has a longer dosing regimen compared to the corresponding chirally uncontrolled oligonucleotide composition. In some embodiments, a chirally controlled oligonucleotide composition has a shorter time period between at least two doses compared to the corresponding chirally uncontrolled oligonucleotide composition. Without wishing to be limited by theory, Applicant notes that in some embodiments longer dosing regimen, and/or shorter time periods between doses, may be due to the improved safety of a chirally controlled oligonucleotide composition. 
     A single dose can contain various amounts of a type of chirally controlled oligonucleotide, as desired suitable by the application. In some embodiments, a single dose contains about 1, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300 or more (e.g., about 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000 or more) mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 1 mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 5 mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 10 mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 15 mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 20 mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 50 mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 100 mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 150 mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 200 mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 250 mg of a type of chirally controlled oligonucleotide. In some embodiments, a single dose contains about 300 mg of a type of chirally controlled oligonucleotide. In some embodiments, a chirally controlled oligonucleotide is administered at a lower amount in a single dose, and/or in total dose, than a chirally uncontrolled oligonucleotide. In some embodiments, a chirally controlled oligonucleotide is administered at a lower amount in a single dose, and/or in total dose, than a chirally uncontrolled oligonucleotide due to improved efficacy. In some embodiments, a chirally controlled oligonucleotide is administered at a higher amount in a single dose, and/or in total dose, than a chirally uncontrolled oligonucleotide. In some embodiments, a chirally controlled oligonucleotide is administered at a higher amount in a single dose, and/or in total dose, than a chirally uncontrolled oligonucleotide due to improved safety. 
     Biologically Active Oligonucleotides 
     A provided oligonucleotide composition as used herein may comprise single stranded and/or multiply stranded oligonucleotides. In some embodiments, single-stranded oligonucleotides contain self-complementary portions that may hybridize under relevant conditions so that, as used, even single-stranded oligonucleotides may have at least partially double-stranded character. In some embodiments, an oligonucleotide included in a provided composition is single-stranded, double-stranded, or triple-stranded. In some embodiments, an oligonucleotide included in a provided composition comprises a single-stranded portion and a multiple-stranded portion within the oligonucleotide. In some embodiments, as noted above, individual single-stranded oligonucleotides can have double-stranded regions and single-stranded regions. 
     In some embodiments, provided compositions include one or more oligonucleotides fully or partially complementary to strand of: structural genes, genes control and/or termination regions, and/or self-replicating systems such as viral or plasmid DNA. In some embodiments, provided compositions include one or more oligonucleotides that are or act as siRNAs or other RNA interference reagents (RNAi agents or iRNA agents), shRNA, antisense oligonucleotides, self-cleaving RNAs, ribozymes, fragment thereof and/or variants thereof (such as Peptidyl transferase 23S rRNA, RNase P, Group I and Group II introns, GIR1 branching ribozymes, Leadzyme, Hairpin ribozymes, Hammerhead ribozymes, HDV ribozymes, Mammalian CPEB3 ribozyme, VS ribozymes, glmS ribozymes, CoTC ribozyme, etc.), microRNAs, microRNA mimics, supermirs, aptamers, antimirs, antagomirs, Ul adaptors, triplex-forming oligonucleotides, RNA activators, long non-coding RNAs, short non-coding RNAs (e.g., piRNAs), immunomodulatory oligonucleotides (such as immunostimulatory oligonucleotides, immunoinhibitory oligonucleotides), GNA, LNA, ENA, PNA, TNA, morpholinos, G-quadruplex (RNA and DNA), antiviral oligonucleotides, and decoy oligonucleotides. 
     In some embodiments, provided compositions include one or more hybrid (e.g., chimeric) oligonucleotides. In the context of the present disclosure, the term “hybrid” broadly refers to mixed structural components of oligonucleotides. Hybrid oligonucleotides may refer to, for example, (1) an oligonucleotide molecule having mixed classes of nucleotides, e.g., part DNA and part RNA within the single molecule (e.g., DNA-RNA); (2) complementary pairs of nucleic acids of different classes, such that DNA:RNA base pairing occurs either intramolecularly or intermolecularly; or both; (3) an oligonucleotide with two or more kinds of the backbone or internucleotide linkages. 
     In some embodiments, provided compositions include one or more oligonucleotide that comprises more than one classes of nucleic acid residues within a single molecule. For example, in any of the embodiments described herein, an oligonucleotide may comprise a DNA portion and an RNA portion. In some embodiments, an oligonucleotide may comprise a unmodified portion and modified portion. 
     Provided oligonucleotide compositions can include oligonucleotides containing any of a variety of modifications, for example as described herein. In some embodiments, particular modifications are selected, for example, in light of intended use. In some embodiments, it is desirable to modify one or both strands of a double-stranded oligonucleotide (or a double-stranded portion of a single-stranded oligonucleotide). In some embodiments, the two strands (or portions) include different modifications. In some embodiments, the two strands include the same modifications. One of skill in the art will appreciate that the degree and type of modifications enabled by methods of the present invention allow for numerous permutations of modifications to be made. Exemplary such modifications are described herein and are not meant to be limiting. 
     RNA Interference 
     Provided oligonucleotide compositions are useful, among other things, for applications in RNA interference. 
     RNA interference (RNAi) refers to the inhibition of gene expression by RNA molecules. Typically, these are small, double-stranded RNA molecules. Since gene expression controls most cellular processes, the ability to inhibit gene expression provides a potentially powerful tool for modulating biological conditions, including treating human and/or animal (e.g., livestock or pet) diseases. A number of studies have been conducted to demonstrate the use of RNAi in regulating or controlling disease-associated gene expression. See, for example: Cullen, K. A., Hall, M. J. &amp; Golosinskiy, A. Ambulatory surgery in the United States, 2006 . Natl Health Stat Report  2009; 1-25; Elbashir S, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001; 411: 494-498; Fire A, Xu S, Montgomery M K, Kostas S A, Driveri S E &amp; Mello C. Potent and specific RNA interference by double-stranded RNA in  Caenorhadbditis elegans. Nature  1998; 391 (6669):806-811; Gauglitz, G. G., Kortin, H. C., Pavicic, T., Ruzicka, T., &amp; Jeschke, M. G. Hypertrophic scarring and keloids: pathomechanisms and current emerging treatment strategies.  Mol Med  2011; 17(1-2): 113-125; Li-Tsang, C. W., Lau, J. C. &amp; Chan, C. C. Prevalence of hypertrophic scar formation and its characteristics among the Chinese population.  Burns  2005; 31, 610-616; Wang H, Ghosh A, Baigude H, Yang C, Qui L, Xia L, et al. Therapeutic gene silencing delivered by a chemically modified siRNA against mutant SOD1 slows ALS progression.  JBC  2008; 283 (23):15845-15852; Weiser, T. G., Regenbogen, S. E., Thompson, K. D., Haynes, A. B., Lipsitz, S. R., Berry, W. R. &amp; Gawande, A. A. An estimation of the global volume of surgery: a modeling strategy based on available data.  Lancet  2008; 372(9633): 139-44. 
     The phenomenon of RNA interference was initially demonstrated in  C. elegans , in which the injection of dsRNA molecules inhibited complementary gene expression. Though the use of siRNA has become a widely used tool for down-regulating gene expression, the existence of a naturally occurring pathway in eukaryotes has been well described. The origin of endogenous siRNA (or miRNA) may be transposons, viruses, repetitive sequences and genes. The process of producing effective endogenous siRNA is regulated by three enzymes. RNA-dependent RNA polymerases convert single-stranded RNA into double-stranded RNA. Alternatively, DNA-dependent RNA polymerases produce dsRNA by transcribing inverted DNA repeats. The resulting large RNA molecules are subject to digestion by ribonuclease III (Dicer) to produce short double-stranded siRNA molecules. Argonaute proteins are then required to bind siRNA molecules to form a complex known as RISC (RNA-induced silencing complex). RISC recognizes double-stranded RNA fragment and splits the double-strands apart, retaining one strand in the RISC complex. RISCs may then promote epigentic silencing through RNA-directed DNA methylation or by target RNA cleavage. Though protein translation may be knocked down considerably, siRNA does not normally eliminate the expression of a gene target completely. RISC can therefore help the guide strand of RNA bind to and destroy its corresponding cellular messenger RNA target. Thus, RNAi provides a method to potentially block the creation of the proteins that cause disease. 
     siRNA technology represents a useful molecular tool. The use of RNA interference for artificially manipulating gene expression was initially limited by the activation of cellular antiviral mechanisms. Exposure of cells to sequences longer than 30 nucleotides has been shown to induce interferon gene expression resulting in non-specific RNA degradation and reduced protein synthesis. However, this problem can be circumvented by designing short (e.g., 19 to 22 nucleotide) siRNA sequences. Methods for siRNA delivery into cells include, without limitation, liposome-based addition of purified ribonucleotides to the media or transfection of plasmid vectors designed to express siRNA molecules. Plasmid vectors rely on the use of two RNA Polymerase III promoters (U6 and H1) to drive transcription of the siRNA molecule. The target sequence (19 to 29 nucleotides) is placed in a sense and antisense orientation with a small spacer group in between (short hairpin RNA or shRNA). Once transcribed, a hairpin structure is formed that can be recognized and cleaved by Dicer. Alternatively, RNA duplexes may be transcribed without hairpin structures and directly process by the RISC. Currently, there are a variety of plasmid and viral vectors that utilize similar concepts to produce siRNA, shRNA, or single stranded siRNA (ss-siRNA) molecules (See, e.g., 2012 Cell-150-883 Walt Lima et al. ssRNAi activate RNAi in animals). 
     In some embodiments, a provided oligonucleotide or oligonucleotide composition is useful as ss-siRNA or GalNAc conjugated siRNA. 
     The art is familiar with certain structural features that affect siRNA as a tool. Following the discovery of siRNA, several studies attempted to identify the optimal characteristics required for siRNA design. Some of the requirements include using sequences shorter than 30 nucleotides to avoid PKR activation, sequence stability at the 5′ end of the antisense strand relative to the 3′ terminus and inserting a TT overhang. Based on studies like these, a number of algorithms have been developed by academic and industrial labs to predict the most effective target sequences for a given gene. Though most of these programs are not perfect, the likelihood of obtaining a predicted sequence is superior to designing sequences without consideration of the recommended features. Synthesis and testing of multiple sequences may be required. The design of siRNA experiments may contain some potential pitfalls, thus the design should be done to include appropriate controls and measurable endpoints. A negative control may include a non-complementary sequence with thermodynamically similar properties as the effective siRNA sequence. When transfecting a plasmid vector to introduce siRNA or shRNA, the ratio of lipid to nucleic acid may be equal and the control vector may contain a sequence that is transcribed and processed intracellularly. Validation of the siRNA effect may also be carried out by measuring both RNA and protein expression. 
     In some embodiments, a provided oligonucleotide as used herein is double-stranded. Typically, double-stranded oligonucleotides comprising a duplex structure of between 20 and 23, but specifically 21, base pairs have been hailed as particularly effective in inducing RNA interference (Elbashir ct al., EMBO 2001, 20:6877-6888). However, others have found that shorter or longer double-stranded oligonucleotides can be effective as well. 
     In some embodiments, a double-stranded oligonucleotide utilized in accordance with the present invention comprises two oligonucleotide strands that are sufficiently complementary to hybridize to form a duplex structure. In some embodiments, a duplex structure is between about 12 to about 45 base pairs in length. In some embodiments, a duplex structure is between about 18 to about 25 base pairs in length. In some embodiments, a duplex structure is between about 19 to about 24 base pairs in length. In some embodiments, a duplex structure is between about 19 to about 21 base pairs in length. In some embodiments, a duplex structure is a double-stranded oligonucleotides of between about 25 to about 30 base pairs in length. In some embodiments, a duplex structure is a double-stranded oligonucleotide of between about 10 to about 15 base pairs in length. In some embodiments, a double-stranded oligonucleotide is at least about 21 nucleotides long. 
     In some embodiments, a double-stranded oligonucleotide utilized in accordance with the present invention comprises a sense strand and an antisense strand, wherein the antisense RNA strand has a region of complementarity which is complementary to at least a part of a target sequence, and the duplex region is about 14 to about 30 nucleotides in length. In some embodiments, a region of complementarity to the target sequence is between about 14 to about 30 nucleotides in length. In some embodiments, a region of complementarity to the target sequence is between about 18 to about 25 nucleotides in length. In some embodiments, a region of complementarity to the target sequence is between about 19 to about 24 nucleotides in length. In some embodiments, a region of complementarity to the target sequence is about 19 to about 21 nucleotides in length. 
     The phrase “antisense strand” as used herein, refers to an oligonucleotide that is substantially or 100% complementary to a target sequence of interest. The phrase “antisense strand” includes the antisense region of both oligonucleotides that are formed from two separate strands, as well as unimolecular oligonucleotides that are capable of forming hairpin or dumbbell type structures. The terms “antisense strand” and “guide strand” are used interchangeably herein. 
     The phrase “sense strand” refers to an oligonucleotide that has the same nucleoside sequence, in whole or in part, as a target sequence such as a messenger RNA or a sequence of DNA. The terms “sense strand” and “passenger strand” are used interchangeably herein. 
     By “target sequence” is meant any nucleic acid sequence whose expression or activity is to be modulated. The target nucleic acid can be DNA or RNA, such as endogenous DNA or RNA, viral DNA or viral RNA, or other RNA encoded by a gene, virus, bacteria, fungus, mammal, or plant. In some embodiments, a target sequence is associated with a disease or disorder. 
     By “specifically hybridizable” and “complementary” is meant that a nucleic acid can form hydrogen bond(s) with another nucleic acid sequence by either traditional Watson-Crick or other non-traditional types. In reference to the nucleic molecules of the present invention, the binding free energy for a nucleic acid molecule with its complementary sequence is sufficient to allow the relevant function of the nucleic acid to proceed, e.g., RNAi activity. Determination of binding free energies for nucleic acid molecules is well known in the art (see, e.g., Turner et al, 1987 , CSH Symp. Quant. Biol . LIT pp. 123-133; Frier et al., 1986 , Proc. Nat. Acad. Sci . USA 83:9373-9377; Turner et al., 1987 , /. Ain. Chem. Soc.  109:3783-3785) 
     A percent complementarity indicates the percentage of contiguous residues in a nucleic acid molecule that can form hydrogen bonds (e.g., Watson-Crick base pairing) with a second nucleic acid sequence (e.g., 5, 6, 7, 8, 9, 10 out of 10 being 50%, 60%, 70%, 80%, 90%, and 100% complementary). “Perfectly complementary” or 100% complementarity means that all the contiguous residues of a nucleic acid sequence will hydrogen bond with the same number of contiguous residues in a second nucleic acid sequence. Less than perfect complementarity refers to the situation in which some, but not all, nucleoside units of two strands can hydrogen bond with each other. “Substantial complementarity” refers to polynucleotide strands exhibiting 90% or greater complementarity, excluding regions of the polynucleotide strands, such as overhangs, that are selected so as to be noncomplementary. Specific binding requires a sufficient degree of complementarity to avoid non-specific binding of the oligomeric compound to non-target sequences under conditions in which specific binding is desired, e.g., under physiological conditions in the case of in vivo assays or therapeutic treatment, or in the case of in vitro assays, under conditions in which the assays are performed. In some embodiments, non-target sequences differ from corresponding target sequences by at least 5 nucleotides. 
     Double Stranded Oligonucleotides 
     In some embodiments, a double-stranded oligonucleotide utilized in accordance with the present invention is sufficiently large that it can be cleaved by an endogenous molecule, e.g., by Dicer, to produce smaller double-stranded oligonucleotides, e.g., RNAi agents. In some embodiments, a provided double-stranded oligonucleotide modulates the expression of a target gene via RISC mediated cleavage of the target sequence. 
     In some embodiments, a double-stranded region of a double-stranded oligonucleotide is equal to or at least, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 23, 24, 25, 26, 27, 28, 29, or 30 nucleotide pairs in length. 
     In some embodiments, an antisense strand of a double-stranded oligonucleotide is equal to or at least 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 23, 24, 25, 26, 27, 28, 29, or 30 nucleotides in length. 
     In some embodiments, a sense strand of a double-stranded oligonucleotide is equal to or at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 23, 24, 25, 26, 27, 28, 29, or 30 nucleotides in length. 
     In some embodiments, one strand has at least one stretch of 1-5 single-stranded nucleotides in the double-stranded region. By “stretch of single-stranded nucleotides in the double-stranded region” is meant that there is present at least one nucleotide base pair at both ends of the single-stranded stretch. In some embodiments, both strands have at least one stretch of 1-5 (e.g., 1, 2, 3, 4, or 5) single-stranded nucleotides in the double stranded region. When both strands have a stretch of 1-5 (e.g., 1, 2, 3, 4, or 5) single-stranded nucleotides in the double stranded region, such single-stranded nucleotides can be opposite to each other (e.g., a stretch of mismatches) or they can be located such that the second strand has no single-stranded nucleotides opposite to the single-stranded oligonucleotides of the first strand and vice versa (e.g., a single-stranded loop). In some embodiments, the single-stranded nucleotides are present within 8 nucleotides from either end, for example 8, 7, 6, 5, 4, 3, or 2 nucleotide from either the 5′ or 3′ end of the region of complementarity between the two strands. 
     In some embodiments, each strand of a double-stranded oligonucleotide utilized in accordance with the present invention has a ZXY structure, such as is described in International Application No. PCT/US2004/07070 filed on Mar. 8, 2004, contents of which are hereby incorporated in their entireties. 
     Hairpins and Dumbbells 
     In some embodiments, a double-stranded oligonucleotide utilized in accordance with the present invention is a single molecule that comprises self-complementary regions; thus the two “strands” of a double-stranded regions are in fact covalently linked to one another. Such two strands can be linked to each other at both ends, or at one end only. By linking at one end is meant that 5′-end of first strand is linked to the 3′-end of the second strand or 3′-end of first strand is linked to 5′-end of the second strand. When the two strands are linked to each other at both ends, 5′-end of first strand is linked to 3′-end of second strand and 3′-end of first strand is linked to 5′-end of second strand. In some embodiments, two strands are linked together by an oligonucleotide linker including, but not limited to, (N) n ; wherein N is independently a modified or unmodified nucleotide and n is 3-23. In some embodiments, n is 3-10, e.g., 3, 4, 5, 6, 7, 8, 9, or 10. In some embodiments, an oligonucleotide linker is selected from the group consisting of GNRA, (G) 4 , (U) 4 , and (dT) 4 , wherein N is a modified or unmodified nucleotide and R is a modified or unmodified purine nucleotide. In some embodiments, some of the nucleotides in the linker are involved in base-pair interactions with other nucleotides in the linker. In some embodiments, the two strands are linked together by a non-nucleosidic linker, e.g. 
     In some embodiments, hairpin and dumbbell type RNAi agents have a duplex region equal to or at least 14, 15, 15, 16, 17, 18, 19, 29, 21, 22, 23, 24, or 25 nucleotide pairs. In some embodiments, the duplex region is equal to or fewer than 200, 100, or 50, nucleotide pairs in length. In some embodiments, ranges for the duplex region are about 15 to about 30, about 17 to about 23, about 19 to about 23, and about 19 to about 21 nucleotides pairs in length. In some embodiments, hairpin oligonucleotides mimic the natural precursors of microRNAs. 
     In some embodiments, hairpin RNAi agents can have a single strand overhang or terminal unpaired region, e.g., at the 3′ end on the antisense side of the hairpin, etc. In some embodiments, the overhangs are about 1 to about 4 nucleotides in length. In some embodiments, the overhangs are about 2 to about 3 nucleotides in length. 
     In some embodiments, a hairpin RNAi agent is characterized in that the 3′-end of an antisense strand is linked to 5′-end of a sense strand. In some embodiments, a hairpin RNAi agent is characterized in that the 5′-end of an antisense strand is linked to the 3′-end of a sense strand. Provided hairpin oligonucleotides are also referred to herein as “shRNA”. 
     Single-Stranded Oligonucleotides 
     In some embodiments, a single-stranded oligonucleotide utilized in accordance with the present invention comprises a nucleotide sequence that is substantially complementary to a “sense” nucleic acid encoding a gene expression product, e.g., complementary to the coding strand of a double-stranded cDNA molecule or complementary to an RNA sequence, e.g., a pre-mRNA, mRNA, miRNA, or premiRNA. Provided single-stranded oligonucleotides include, but are not limited to, antisense oligonucleotides and single-stranded RNAi agents. In some embodiments, the region of complementarity is less than about 30 nucleotides in length. In some embodiments, the region of complementarity is at least about 15 nucleotides in length. In some embodiments, a provided single stranded oligonucleotide is about 10 to about 25 nucleotides in length (e.g., about 11, 12, 13, 14, 15, 16, 18, 19, 20, 21, 22, 23, or 24 nucleotides in length). In some embodiments, a provided single stranded oligonucleotide is about 25 to about 30 nucleotides in length. In some embodiments, a provided single stranded oligonucleotide is about 15 to about 29 nucleotides in length. In some embodiments, a provided single stranded oligonucleotide is characterized as having less than 100% complementarity to a mRNA, RNA or DNA arc. In some embodiments, a provided single-stranded oligonucleotide has a ZXY structure, such as is described in International Application No. PCT/US2004/07070 filed on Mar. 8, 2004. 
     In some embodiments, a utilized single-stranded oligonucleotide can hybridize to a complementary RNA, e.g., mRNA, pre-mRNA, and prevent access of the translation machinery to the target RNA transcript, thereby preventing protein synthesis. In some embodiments, a provided single-stranded oligonucleotide can hybridize to a complementary RNA and the RNA target can be subsequently cleaved by an enzyme such as RNasc H, thus preventing translation of target RNA. In some embodiments, a provided single-stranded oligonucleotide modulates the expression of a target gene via RISC mediated cleavage of the target sequence. 
     A “single-stranded RNAi agent” as used herein, is an RNAi agent which is made up of a single molecule. In some embodiments, a single-stranded RNAi agent includes a duplexed region, formed by intra-strand pairing, e.g., it is or it includes a hairpin or pan-handle structure. In some embodiments, single-stranded RNAi agents are antisense with regard to the target molecule. In some embodiments, single-stranded RNAi agents are sufficiently long such that they are able to enter the RISC and participate in RISC mediated cleavage of a target mRNA. Exemplary single-stranded siRNAs (ss siRNAs) are known and are described, for example, in U.S. Pat. Pub. No. 2006/0166901, the contents of which are herein incorporated by reference in its entirety. 
     In some embodiments, a single-stranded RNAi agent is at least about 12 nucleotides in length. In some embodiments, a single-stranded RNAi agent is at least about 15 nucleotides in length. In some embodiments, a single-stranded RNAi agent is at least about 20 nucleotides in length. In some embodiments, a single-stranded RNAi agent is at least about 25 nucleotides in length. In some embodiments, a single-stranded RNAi agent is at least about 29 nucleotides in length. In some embodiments, a single-stranded RNAi agent is at least about 30 nucleotides in length. In some embodiments, a single-stranded RNAi agent is at least about 35 nucleotides in length. In some embodiments, a single-stranded RNAi agent is at least about 40 nucleotides in length. In some embodiments, a single-stranded RNAi agent is at least about 50 nucleotides in length. 
     In some embodiments, a single-stranded RNAi agent is less than 200 nucleotides in length. In some embodiments, a single-stranded RNAi agent is less than 100 nucleotides in length. In some embodiments, a single-stranded RNAi agent is less than 60 nucleotides in length. 
     In some embodiments, a single-stranded RNAi agent is 5′ phosphorylated. In some embodiments, a singlestranded RNAi agent includes a phosphoryl analog at the 5′ prime terminus. In certain embodiments, a single-stranded RNAi agent has length from about 15 to about 29 nucleotides in length. 
     Single-stranded oligonucleotides, including those described and/or identified as single stranded siRNAs, microRNAs or mirs which may be used as targets or may serve as a template for the design of oligonucleotides of the invention are taught in, for example, Esau, et al. US Publication #20050261218 (U.S. Ser. No. 10/909,125) entitled “Oligonucleotides and compositions for use in modulation small non-coding RNAs” the entire contents of which are incorporated herein by reference. 
     The present invention encompasses research and/or diagnostic reagents that comprise single-stranded oligonucleotides. In some embodiments, a single-stranded oligonucleotide utilized in accordance with the present invention is and/or acts as a primer. In some embodiments, primers are used in polymerase-based chain reactions (i.e., PCR) to amplify nucleic acids. These applications include any known variations of PCR, such as reverse transcription PCR (RT-PCR) and real-time PCR. 
     MicroRNAs 
     In some embodiments provided compositions comprise one or more oligonucleotides that are or act as MicroRNAs. 
     MicroRNAs (miRNAs or mirs) are a highly conserved class of small RNA molecules that are transcribed from DNA in the genomes of plants and animals, but are not translated into protein. Pre-microRNAs are processed into miRNAs. Processed microRNAs are single stranded 17-25 nucleotide (nt) RNA molecules that become incorporated into the RNA-induced silencing complex (RISC) and have been identified as key regulators of development, cell proliferation, apoptosis and differentiation. They are believed to play a role in regulation of gene expression by binding to the 3′-untranslated region of specific mRNAs. RISC mediates downregulation of gene expression through translational inhibition, transcript cleavage, or both. RISC is also implicated in transcriptional silencing in the nucleus of a wide range of eukaryotes. 
     MicroRNAs have also been implicated in modulation of pathogens in hosts. For example, see Jopling, C. L., et al.,  Science  (2005) vol. 309, pp 1577-1581. Without wishing to be bound by theory, administration of a microRNA, microRNA mimic, and/or anti microRNA oligonucleotide, leads to modulation of pathogen viability, growth, development, and/or replication. In some embodiments, a provided oligonucleotide is a microRNA, microRNA mimic, and/or anti microRNA, wherein microRNA is a host microRNA. The number of miRNA sequences identified to date is large and growing, illustrative examples of which can be found, for example, in: “ miRBase: microRIVA sequences, targets and gene nomenclature ” Griffiths-Jones S, Grocock R J, van Dongen S, Bateman A, Enright A J. NAR, 2006, 34, Database Issue, D140-D144 ; “The microRNA Registry ” Griffiths-Jones S. NAR, 2004, 32, Database Issue, D109-D111. Non-limiting examples of useful miRNA sequences are also provided in the accompanying Appendix (C). 
     Ribozymes 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as ribozymes. 
     Ribozymes are oligonucleotides having specific catalytic domains that possess endonuclease activity (Kim and Cech, Proc Natl Acad Sci USA. 1987 December; 84(24):8788-92; Forster and Symons, Cell. 1987 Apr. 24; 49(2):211-20). At least six basic varieties of naturally occurring enzymatic RNAs are known presently. In general, enzymatic nucleic acids act by first binding to a target RNA. Such binding occurs through the target binding portion of an enzymatic nucleic acid which is held in close proximity to an enzymatic portion of the molecule that acts to cleave the target RNA. Thus, the enzymatic nucleic acid first recognizes and then binds a target RNA through complementary base-pairing, and once bound to the correct site, acts enzymatically to cut the target RNA. Strategic cleavage of such a target RNA will destroy its ability to direct synthesis of an encoded protein. After an enzymatic nucleic acid has bound and cleaved its RNA target, it is released from that RNA to search for another target and can repeatedly bind and cleave new targets. 
     Methods of producing a ribozyme targeted to any target sequence are known in the art. Ribozymes can be designed as described in, inter alia, Int. Pat. Appl. Publ. No. WO 93/23569 and Int. Pat. Appl. Publ. No. WO 94/02595, each specifically incorporated herein by reference, and synthesized to be tested in vitro and in vivo, as described therein. 
     Aptamers 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as aptamers. 
     Aptamers are nucleic acid or peptide molecules that bind to a particular molecule of interest with high affinity and specificity (Tuerk and Gold, Science 249:505 (1990); Ellington and Szostak, Nature 346:818 (1990)). DNA or RNA aptamers have been successfully produced which bind many different entities from large proteins to small organic molecules. See Eaton, Curr. Opin. Chem. Biol. 1:10-16 (1997), Famulok, Curr. Opin. Struct. Biol. 9:324-9(1999), and Hermann and Patel, Science 287:820-5 (2000). Aptamers can be RNA or DNA based. Generally, aptamers are engineered through repeated rounds of in vitro selection or equivalently, SELEX (systematic evolution of ligands by exponential enrichment) to bind to various molecular targets such as small molecules, proteins, nucleic acids, and even cells, tissues and organisms. The SELEX procedure is a protocol in which single stranded oligonucleotides are selected from vast libraries of sequences, based on binding affinity at a target protein or other molecule (C. Tuerk, L. Gold, Science, 249 (1990), pp. 505-510; R. Green, A. D. Ellington, D. P. Bartel, J. W. Szostak, Methods Enzymol., 2 (1991), pp. 75-86; L. Gold, B. Polisky, O. Uhlenbeck, M. Yarus, Annu. Rev. Biochem., 64 (1995), pp. 763-797). The SELEX procedure is usually initiated with an RNA or DNA library consisting of some 10 14 -10 15  random oligonucleotide sequences. In a fully randomised oligonucleotide library, each molecule will exhibit a unique tertiary structure which will be dependent on the nucleotide sequence of that molecule. The binding affinity of the oligonucleotide for the target protein will be determined by the fit between moieties on the surface of the oligonucleotide and epitopes on the target protein. As a consequence of starting from a library of vast diversity it is often possible to identify aptamers of nM or sub-nM affinity for the target protein and with selectivity for that target protein over other proteins with a high degree of structural homology (K. W. Uphoff, S. D. Bell, A. D. Ellington, Curr. Opin. Struct. Biol., 6 (1996), pp. 281-288). Using SELEX methodology RNA or DNA aptamers have been generated to many proteins, peptides and small molecules including dopamine (C. Mannironi, A. Di Nardo, P. Fruscoloni, G. P. Tocchini-Valentini, Biochemistry, 36 (1997), pp. 9726-9734), substance P (D. Nieuwlandt, M. Wecker, Biochemistry, 34 (1995), pp. 5651-5659), subtilisin (H. Takeno, S. Yamamoto, T. Tanaka, Y. Sakano, Y. Kikuchi, J. Biochem., 125 (1999), pp. 1115-1119), platelet derived growth factor (L. S. Green, D. Jellinek, R. Jenison, A. Ostman, C-H. Heldin, N. Janjic Biochemistry, 35 (1996), pp. 14413-14424), vascular endothelial growth factor (L. S. Green, D. Jellinek, C. Bell, L. A. Bebe, B. D. Feistner, S. C. Gill, F. M. Jucker, N. Janjic Chem. Biol., 2 (1995), pp. 683-695), thrombin (L. C. Bock, L. C. Griffen, J. A. Latham, E. H. Vermaas, J. J. Toole, Nature, 355 (1992), pp. 564-566), and L-selectin (D. O&#39;Connell, A. Koenig, S. Jennings, B. Hicke, H. L. Han, T. Fitzwater, Y. F. Chang, N. Varki, D. Parma Proc. Natl. Acad. Sci. USA, 93 (1996), pp. 5883-5887). 
     As reviewed in Dua et al. (2008) Recent Patents on DNA &amp; Gene Sequences, 2: 172-186 (incorporated herein by reference) in more detail, over the years, a number of modified SELEX protocols have been developed. Non-limiting examples of modified SELEX methods are described in the following publications: Counter SELEX (U.S. Pat. No. 5,580,737), Flow cell SELEX (WO9833941), Truncation SELEX (WO0056930), Blended SELEX (U.S. Pat. No. 5,683,867), Transcription free SELEX (US20026387620), Solution SELEX (U.S. Pat. No. 5,567,588), Chimeric SELEX (WO9604403), Tissue SELEX (US20026376474), Photo SELEX (U.S. Pat. No. 6,001,577), Toggle SELEX (US20077312325), Covalent SELEX/Chemi SELEX (U.S. Pat. No. 5,763,595), Genomic SELEX (US20016261774), SELEX without purified protein (WO0224954), CE-SELEX (WO03102212), Mirror-image SELEX—Spiegelmers (EP1386972) and Laser SELEX, DeSELEX (WO07035532), each of which is incorporated herein by reference in its entirety. Stereo-defined oligonucleotides embraced by the present invention may be used in any one or more of the variations of the SELEX methods. 
     An aptamer can be prepared by any known method, including synthetic, recombinant, and purification methods, and can be used alone or in combination with other aptamers specific for the same target. Further, as described more fully herein, the term “aptamer” specifically includes “secondary aptamers” containing a consensus sequence derived from comparing two or more known aptamers to a given target. Aptamers possess several characteristics that make them attractive therapeutic agents. Both DNA and RNA aptamers have been shown to bind their targets with dissociation constants (Kd) in the low picomolar to low nanomolar range. Binding of an aptamer is a highly specific interaction that can even discriminate between related proteins that share common structural domains. Though the binding affinity of both aptamers and antibodies is in the same range, aptamers have many additional features which overpower their rival in many cases. Unlike antibodies, aptamers can be targeted even against non immunogenic targets. During synthesis they can be easily subjected to a chemical modification that improves their stability and pharmacokinetics. They display nil to negligible levels of immunogenicity at therapeutic doses due to their resemblance to endogenous molecules. 
     In some embodiments, oligonucleotides utilized in accordance with the present invention are useful as anti-pathogenic agents, for example, antiviral agents, antibacterial agents, antifungal agents, and so on. Suitable targets for a variety of infectious agents such as viruses and bacteria have been reported. See, for example, U.S. Pat. Nos. 5,726,017, 5,654,151, 5,496,938, 5,503,978, 5,587,468, 5,527,894, US2005233317, U.S. Pat. No. 5,496,938, WO08 066231, JP2002165594, WO02081494, U.S. Pat. No. 5,861,501, WO9720072, U.S. Pat. Nos. 5,475,096, 6,569,630, CN101109013, and WO03106476, each of which is incorporated herein by reference in its entirety. 
     In some embodiments, oligonucleotides utilized in accordance with the present invention are or act as anticancer agents. Any known cancer- or tumor-associated factors, such as proteins involved in the regulation of cell division or proliferation, cell cycle progression, apoptosis, cell migration, DNA repair, etc. including structural proteins and signal transduction molecules, may be targeted by an aptamer. Examples include, without limitation, See, for example, AU Patent 775412, U.S. Pat. No. 6,232,071, WO 08/028534, U.S. Pat. No. 6,933,114, WO 04/081574, AU Patent 242462, U.S. Pat. Nos. 6,995,249, 5,668,264, 6,699,843, WO 04/094614, and U.S. Pat. No. 5,846,713, each of which is incorporated herein by reference in its entirety. 
     In some embodiments, oligonucleotides utilized in accordance with the present invention are or act as antiangiogenic agents. Non-limiting examples of antiangiogenic targets include VEGF and associates receptors, as well as extracellular matrix or adhesion molecules. See, for example, U.S. Pat. No. 6,051,698, US 2003175703, WO 95/21853 and U.S. Pat. No. 7,094,535, each of which is incorporated herein by reference in its entirety. 
     In some embodiments, oligonucleotides utilized in accordance with the present invention are or act as anticoagulant agents. Much is known about blood coagulation factors and their function as well as proteins regulation such process. Aptamers are useful in targeting one or more of such factors in treating conditions such as cardiovascular diseases and blood coagulation disorders. See for example, WO 06/033854, U.S. Pat. No. 5,543,293, WO 07/025049, U.S. Pat. No. 6,774,118, WO 07/140000, each of which is incorporated herein by reference in its entirety. 
     In some embodiments, oligonucleotides utilized in accordance with the present invention are or act as immunomodulatory agents. Aptamers have been generated to target immunomodulatory molecules involved in autoimmune disorders. The present invention may be useful for targeting molecules expressed on immune cells, such as T cells and antigen presenting cells (APCs). See for example, U.S. Pat. No. 5,869,641, WO 01/09160, each of which is incorporated herein by reference in its entirety. In some embodiments, molecules involved in the complement system such as C1, C4, C2, C3 and C5 may be targeted by an aptamer. The complement system has been implicated in numerous renal, rheumatological, neurological, dermatological, hematological, allergic, infectious, and other diseases. See for example, WO 97/28178 and WO 07/103549, each of which is incorporated herein by reference in its entirety. Other immune-related targets include, without limitation, IL-12, IL-23 and IL-28 (see, for example, WO 07/035922, which is incorporated herein by reference), IgE (see, for example, WO 05/113813, which is incorporated herein by reference), Sp-1 and Sp1-, CD28, IL-2, GMCSF (see, for example, U.S. Pat. No. 6,994,959, which is incorporated herein by reference), SDF-1, CXCR4 (see, for example, WO 08/009437, which is incorporated herein by reference), IL-6, IL-12, IFN gamma (see, for example, U.S. Pat. No. 6,589,940 and US2003125279, each of which is incorporated herein by reference), and TLR. Stereo-defined aptamers may elicit improved efficacy against such diseases. 
     In some embodiments, oligonucleotides utilized in accordance with the present invention are or act as anti-inflammatory agents. Inflammatory disease such as acute respiratory distress syndrome (ARDS), septic shock, pulmonary emphysema, cystic fibrosis, rheumatoid arthritis and chronic bronchitis, have neutrophil elastase involved in their pathogenesis. Human elastate is therefore a target for treating such disorders using an aptamer that regulates inflammation. Other suitable targets include, without limitation, phospholipase A2, such as non-pancreatic secretory PLA2 (see, for example, WO 96/27604, which is incorporated by reference), selectins, E-, P- and L- (see, for example, U.S. Pat. No. 5,780,228, which is incorporated by reference), three homologous C-type lectins, other cell adhesion molecules expressed in cells such as leukocytes, endothelial cells and platelets; MCP-1 (see, for example, WO 07/093409, which is incorporated by reference), NF-kappa B and NF-IL6 (see, for example, WO 00/24404, which is incorporated by reference). Stereo-defined aptamers may elicit improved efficacy against such diseases. 
     In some embodiments, oligonucleotides utilized in accordance with the present invention are useful for treating certain brain diseases, including but are not limited to: Transmissible spongiform encephalopathies (TSEs) and Alzheimer&#39;s disease. Known targets are described in publications including WO 2006/138676 and DE19916417, and WO 08/008884, each of which is incorporated herein by reference. Stereo-defined aptamers may elicit improved efficacy against such diseases. 
     Decoy Oligonucleotides 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as decoy oligonucleotides. 
     Because transcription factors recognize their relatively short binding sequences, even in the absence of surrounding genomic DNA, short oligonucleotides bearing the consensus binding sequence of a specific transcription factor can be used as tools for manipulating gene expression in living cells. This strategy involves the intracellular delivery of such “decoy oligonucleotides”, which are then recognized and bound by the target factor. Occupation of the transcription factor&#39;s DNA-binding site by the decoy renders the transcription factor incapable of subsequently binding to the promoter regions of target genes. Decoys can be used as therapeutic agents, either to inhibit the expression of genes that are activated by a transcription factor, or to up-regulate genes that are suppressed by the binding of a transcription factor. Examples of the utilization of decoy oligonucleotides can be found in Mann et al., J. Clin. Invest., 2000, 106: 1071-1075, which is expressly incorporated by reference herein, in its entirety. 
     miRNA Mimics 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as miRNA mimics. 
     miRNA mimics represent a class of molecules that can be used to imitate the gene modulating activity of one or more miRNAs. Thus, the term “microRNA mimic” refers to synthetic non-coding RNAs (i.e. the miRNA is not obtained by purification from a source of the endogenous miRNA) that are capable of entering the RNAi pathway and regulating gene expression. miRNA mimics can be designed as mature molecules (e.g. single stranded) or mimic precursors (e.g., pri- or pre-miRNAs). 
     In some embodiments, miRNA mimics are double stranded molecules (e.g., with a duplex region of between about 16 and about 31 nucleotides in length) and contain one or more sequences that have identity with the mature strand of a given miRNA. 
     In some embodiments, an miRNA mimic comprises a duplex region of between about 16 and about 31 nucleotides. In some embodiments, provided miRNA mimic may contain one or more of the following chemical modification patterns: the sense strand contains 2′-O-methyl modifications of nucleotides 1 and 2 (counting from the 5′ end of the sense oligonucleotide), and all of the Cs and Us; the antisense strand modifications can comprise 2′ F modification of all of the Cs and Us, phosphorylation of the 5′ end of the oligonucleotide, and stabilized internucleotide linkages associated with a 2 nucleotide 3′ overhang. 
     Supermirs 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as supermirs. 
     A supermir refers to an oligonucleotide, e.g., single stranded, double stranded or partially double stranded, which has a nucleotide sequence that is substantially identical to an miRNA and that is antisense with respect to its target. This term includes oligonucleotides which comprise at least one non-naturally-occurring portion which functions similarly. In some embodiments, the supermir does not include a sense strand. In some embodiments, the supermir does not self-hybridize to a significant extent. In some embodiments, a supermir has a secondary structure but is substantially single-stranded under physiological conditions. A supermir that is substantially single-stranded is single-stranded to the extent that less than about 50% (e.g., less than about 40%, 30%, 20%, 10%, or 5%) of the supermir is duplexed with itself. A supermir can include a hairpin segment, e.g., sequence, preferably at the 3′ end can self hybridize and form a duplex region, e.g., a duplex region of at least 1, 2, 3, or 4 and preferably less than 8, 7, 6, or 5 nucleotides, e.g., 5 nucleotides. The duplexed region can be connected by a linker, e.g., a nucleotide linker, e.g., 3, 4, 5, or 6 dTs, e.g., modified dTs. In some embodiments, a supermir is duplexed with a shorter oligonucleotide, e.g., of 5, 6, 7, 8, 9, or 10 nucleotides in length, e.g., at one or both of the 3′ and 5′ end or at one end and in the non-terminal or middle of the supermir. 
     Antimers or miRNA Inhibitors 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as antimers or miRNA inhibitors. 
     The terms “antimir” “microRNA inhibitor” or “miR inhibitor” are synonymous and refer to oligonucleotides or modified oligonucleotides that interfere with the activity of specific miRNAs. Inhibitors can adopt a variety of configurations including single stranded, double stranded (RNA/RNA or RNA/DNA duplexes), and hairpin designs. In some embodiments, microRNA inhibitors comprise one or more sequences or portions of sequences that are complementary or partially complementary with the mature strand (or strands) of the miRNA to be targeted. In some embodiments, miRNA inhibitors comprise additional sequences located 5′ and 3′ to the sequence that is the reverse complement of the mature miRNA. Additional sequences can be the reverse complements of the sequences that are adjacent to the mature miRNA in the primiRNA from which the mature miRNA is derived, or additional sequences can be arbitrary sequences (having a mixture of A, G, C, U, or dT). In some embodiments, one or both of the additional sequences are arbitrary sequences capable of forming hairpins. Thus, in some embodiments, the sequence that is the reverse complement of the miRNA is flanked on the 5′ side and on the 3′ side by hairpin structures. In some embodiments, microRNA inhibitors are double stranded. In some embodiments, microRNA inhibitors are double stranded and include mismatches between nucleotides on opposite strands. In some embodiments, microRNA inhibitors are linked to conjugate moieties in order to facilitate uptake of the inhibitor into a cell. 
     MicroRNA inhibitors, including hairpin miRNA inhibitors, are described in detail in Vermeulen et al., “Double-Stranded Regions Are Essential Design Components Of Potent Inhibitors of RISC Function,” RNA 13: 723-730 (2007) and in W02007/095387 and WO 2008/036825 each of which is incorporated herein by reference in its entirety. 
     An exemplary application of miRNA-based therapy is described in Pan et al. (2007) World J Gastroenterol 13(33): 4431-36, “New therapeutic opportunities for Hepatitis C based on small RNA,” the contents of which are incorporated by reference. Briefly, the authors describe 22 nucleotide mature miR-122, derived from a noncoding polyadenylated RNA transcript of the_hcr gene, which is a liver-specific developmental regulator. Because miR-122 is a liver specific miRNA that is involved in HCV viral replication, silencing of miR-122 can be useful for the treatment of HCV. 
     Antagomirs 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as antagomirs. 
     Antagomirs are RNA-like oligonucleotides that harbor various modifications for RNAse protection and pharmacologic properties, such as enhanced tissue and cellular uptake. They differ from normal RNA by, for example, complete 2′-0-methylation of sugar, phosphorothioate intersugar linkage and, for example, a cholesterol-moiety at 3′-end. In some embodiments, an antagomir comprises a 2′-O-methylmodification at all nucleotides, a cholesterol moiety at 3′-end, two phosphorothioate intersugar linkages at the first two positions at the 5′-end and four phosphorothioate linkages at the 3′-end of the molecule. Antagomirs can be used to efficiently silence endogenous miRNAs by forming duplexes comprising the antagomir and endogenous miRNA, thereby preventing miRNA-induced gene silencing. An example of antagomir-mediated miRNA silencing is the silencing of miR-122, described in Krutzfeldt et al, Nature, 2005, 438: 685-689, which is incorporated by reference herein in its entirety. 
     Modified Single Oligonucleotide Variations 
     Typically, it is perceived that two components are required to activate RNAi machinery—the double-stranded nucleic acid motif, which is required for recognition by RNAi-associated proteins and the guide strand which serves as the mRNA binding co-factor in the RISC&#39;s Argonaute catalytic protein. More recently, a novel type of RNAi molecules composed of a single, short (25-28 nt) oligo capable of self-dimerizing into a partially complementary duplex have been developed. These molecules were demonstrated to efficiently activate the RISC and to produce target mRNA silencing comparable to that obtained with potent conventional RNAi molecules. See: Lapierre et al., “Potent and systematic RNAi mediated silencing with single oligonucleotide compounds.” RNA 2011; 17:00, the contents of which are hereby incorporated by reference. See also: WO 2010/090762 “RNA DUPLEXES WITH SINGLE STRANDED PHOSPHOROTHIOATE NUCLEOTIDE REGIONS FOR ADDITIONAL FUNCTIONALITY” (PCT/US2010/00348), the contents of which are hereby incorporated by reference. 
     Thus, the present invention includes RNAi constructs containing single stranded regions of phosphorothioate modified nucleotides, and the uses of such constructs in gene silencing. 
     In some embodiments, the invention utilizes isolated double stranded nucleic acid molecules including a guide strand and a passenger strand, wherein the passenger strand is connected through a cleavable linker to a single stranded region of at least eight phosphorothioate modified nucleotides. In some embodiments, the invention is an isolated double stranded nucleic acid molecule having a guide strand and a passenger strand, wherein at least one of the guide strand and passenger strand is connected through a cleavable linker to a single stranded region of at least six phosphorothioate modified nucleotides. 
     In some embodiments, the invention utilizes an isolated double stranded nucleic acid molecule having a guide strand and a passenger strand, wherein at least one of the guide strand and passenger strand is connected through a cleavable linker to a single stranded region of at least three phosphorothioate modified nucleotides. In some embodiments, the double stranded nucleic acid molecule includes at least one of the following properties. The passenger strand may be 8-18 nucleotides in length. The nucleic acid may have at least one 2′ O methyl or 2′ fluoro modification. The cleavable linkage may be other than a nucleotidic linkage. The nucleic acid may include a lipophilic group. The guide strand may be 16-18 nucleotides or 26-28 nucleotides in length. In some embodiments the single stranded region is connected to the guide strand. 
     In some embodiments, the cleavable linker includes one or more unmodified nucleotides. In other embodiments, the cleavable linker is a phosphodiester bond. In certain embodiments, the cleavable linker is S—S. In some embodiments the cleavable linker is DNA or RNA. The single stranded region of at least eight phosphorothioate modified nucleotides can be at either the 3′ or 5′ end of the passenger strand. In some embodiments, the single stranded region of at least eight phosphorothioate modified nucleotides is DNA, while in other embodiments it is RNA. 
     In some embodiments the double stranded region of the nucleic acid molecule is a perfect duplex. In other embodiments the double stranded region contains at least one bulge region. In some embodiments the passenger strand comprises a nick within the double stranded region of the molecule. The double stranded region may contain at least one nucleotide that is phosphorothioate modified. 
     Nucleic acid molecules utilized in accordance with the invention may be chemically modified. In certain embodiments the chemical modification is 2′Omethyl and/or 2′Fluoro. In some embodiments more than one chemical modification is present in the same molecule. In some embodiments chemical modification increases stability, increases evasion of immune regulation, and/or prevents off-target gene silencing. Chemical modification can be present on the passenger strand and/or the guide strand. In some embodiments the single stranded region of at least eight phosphorothioate modified nucleotides is cleaved from the double stranded region of the nucleic acid molecule in a cell. In some embodiments the single stranded region of at least eight phosphorothioate modified nucleotides has complementarity to a mammalian gene. In certain embodiments the single stranded region of at least eight phosphorothioate modified nucleotides functions as an antisense molecule. The double stranded region may be at least 19 nucleotides long. In some embodiments the single stranded region is at least 12 nucleotides long. 
     In some embodiments, the invention utilizes bifunctional nucleic acid molecule including a double stranded region that functions in RNA interference and a single stranded region that functions in antisense, wherein the double stranded region comprises a guide strand and a passenger strand, and wherein the double stranded region and the single stranded region are connected through a cleavable linker. In some embodiments, the cleavable linker includes one or more unmodified nucleotides. In other embodiments, the cleavable linker is a phosphodiester bond. In certain embodiments, the cleavable linker is S—S. In some embodiments the cleavable linker is DNA or RNA. 
     In some embodiments, the invention relates to methods for inhibiting the expression of a target gene in a mammalian cell, including contacting the mammalian cell with an isolated double stranded nucleic acid molecule comprising a guide strand and a passenger strand, wherein the passenger strand is connected through a cleavable linker to a single stranded region of at least eight phosphorothioate modified nucleotides. In some embodiments, the cleavable linker includes one or more unmodified nucleotides. In other embodiments, the cleavable linker is a phosphodiester bond. In certain embodiments, the cleavable linker is S—S. In some embodiments the cleavable linker is DNA or RNA. 
     In some embodiments, the single stranded region comprises at least eight phosphorothioate modified nucleotides and can be at either the 3′ or 5′ end of the passenger strand. In some embodiments, the single stranded region of at least eight phosphorothioate modified nucleotides is DNA, while in other embodiments it is RNA. In some embodiments the double stranded region of the nucleic acid molecule is a perfect duplex. In other embodiments the double stranded region contains at least one bulge region. In some embodiments the passenger strand comprises a nick within the double stranded region of the molecule. The double stranded region may contain at least one nucleotide that is phosphorothioate modified. 
     Nucleic acid molecules utilized in accordance with the invention may be chemically modified. In certain embodiments the chemical modification is 2′Omethyl and/or 2′Fluoro. In some embodiments more than one chemical modification is present in the same molecule. In some embodiments chemical modification increases stability, increases evasion of immune regulation, and/or prevents off-target gene silencing. Chemical modification can be present on the passenger strand and/or the guide strand. 
     In some embodiments the single stranded region of at least eight phosphorothioate modified nucleotides is cleaved from the double stranded region of the nucleic acid molecule in a cell. In some embodiments the single stranded region of at least eight phosphorothioate modified nucleotides has complementarity to a mammalian gene. In certain embodiments the single stranded region of at least eight phosphorothioate modified nucleotides functions as an antisense molecule. The double stranded region may be at least 19 nucleotides long. In some embodiments the single stranded region is at least 12 nucleotides long. 
     In some embodiments, the invention relates to methods for inhibiting the expression of a target gene in a mammalian cell, comprising contacting the mammalian cell with an isolated double stranded nucleic acid molecule comprising a guide strand and a passenger strand, wherein the guide strand is connected through a cleavable linker to a single stranded region of at least eight phosphorothioate modified nucleotides. In some embodiments, the cleavable linker includes one or more unmodified nucleotides. In other embodiments, the cleavable linker is a phosphodiester bond. In certain embodiments, the cleavable linker is S—S. In some embodiments the cleavable linker is DNA or RNA. 
     In some embodiments, the invention relate to methods for inhibiting the expression of a target gene in a mammalian cell, including contacting the mammalian cell with a bifunctional nucleic acid molecule including a double stranded region that functions in RNA interference and a single stranded region that functions in antisense, wherein the double stranded region includes a guide strand and a passenger strand, and wherein the double stranded region and the single stranded region are connected through a cleavable linker. In other aspects a method for inhibiting the expression of a target gene in a mammalian cell is provided. The method involves contacting the mammalian cell with any of the isolated double stranded nucleic acid molecules described herein. 
     In some embodiments, isolated double stranded nucleic acid molecules utilized in accordance with the present invention include a chemical modification that increases stability. In some embodiments, isolated double stranded nucleic acid molecules include a chemical modification that increases evasion of immune regulation. In some embodiments, the isolated double stranded nucleic acid molecules include a chemical modification that prevents off-target gene silencing. 
     In some embodiments, as discussed herein, utilized oligonucleotides are single oligonucleotide molecules capable of self-dimerizing into a partially complementary duplex. In some embodiments, such single oligonucleotides are about 23-30 nucleotides in length, e.g., 23, 24, 25, 26, 27, 28, 29, and 30. In some embodiments, single oligonucleotides capable of self-dimerizing into a partially complementary duplex contain about 14-18 nucleotide mRNA targeting region, e.g., 14, 15, 16, 17, and 18. In some embodiments, single oligonucleotides capable of self-dimerizing into a partially complementary duplex contain an additional 7-11, e.g., 7, 8, 9, 10, and 11 nucleotides to enable self-dimerization into a partially complementary duplex. In some embodiments, single oligonucleotides capable of self-dimerizing into a partially complementary duplex can efficiently enter and activate the RNA-induced silencing complex (RISC). 
     Ul Adaptors 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as U1 adaptors. 
     Ul adaptors inhibit polyA sites and are bifunctional oligonucleotides with a target domain complementary to a site in the target gene&#39;s terminal exon and a ‘Ul domain’ that binds to the Ul smaller nuclear RNA component of the Ul snRNP. See for example, Int. Pat. App. Pub. No. W02008/121963 and Goraczniak, et al., 2008, Nature Biotechnology, 27(3), 257-263, each of which is incorporated by reference herein, in its entirety. Ul snRNP is a ribonucleoprotein complex that functions primarily to direct early steps in spliceosome formation by binding to the pre-mRNA exon-intron boundary, Brown and Simpson, 1998, Annu Rev Plant Physiol Plant MoI Biol 49:77-95. 
     In some embodiments, a utilized oligonucleotide is a Ul adaptor, wherein the oligonucleotide comprises at least one annealing domain (targeting domain) linked to at least one effector domain (U1 domain), wherein the annealing domain hybridizes to a target gene sequence and the effector domain hybridizes to the Ul snRNA of Ul snRNP. In some embodiments, the Ul adaptor comprises one annealing domain. In some embodiments, the Ul adaptor comprises one effector domain. 
     Without wishing to be bound by theory, the annealing domain will typically be from about 10 to about 50 nucleotides in length, more typically from about 10 to about 30 nucleotides or about 10 to about 20 nucleotides. In some embodiments, the annealing, domain is 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or 21 nucleotides in length. The annealing domain may be at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, or at least about 100% complementary to the target gene. In some embodiments, the annealing domain hybridizes with a target site within the Y terminal exon of a pre-MRNA, which includes the terminal coding region and the 3′UTR and polyadenylation signal sequences (e.g., through the polyadenylation site). In some embodiments, the target sequence is within about 500 ‘basepair, about 250 basepair, about 100 basepair, or about 50 ‘basepair of the poly (A) signal sequence of the, pre-mRNA. In some embodiments, the annealing domain comprises 1, 2, 3, or 4, mismatches with the target gene sequence. 
     In some embodiments, the effector domain is from about 8 nucleotides to about 30 nucleotides in length. In some embodiments, the effector domain is from about 10 nucleotides to about 20 nucleotides in length. In some embodiments, the effector domain is from about 10 nucleotides to about 15 nucleotides in length. The U1 domain can hybridize with UI snRNA, particularly the 5′ end and more specifically nucleotides 2-11. In some embodiments, the Ul domain, is perfectly complementary to nucleotides 2-11 of endogenous U1 snRNA. In some embodiments, the U1 domain comprises a nucleotide sequence selected from the group consisting of SEQ. ID NO: 2299 (5′-GCCAGGUAAGUAU-3′), SEQ. ID NO: 2300(5′-CCAGGUAAGUAU-3′). SEQ ID NO: 2301 (5′-CAGGUAAGUAU-3′), SEQ ID NO: 2302 (5′-CAGGUAAGU-3′), SEQ ID NO: 2303 (5′-CAGGUAAG-3′), and. SEQ ID NO: 2304 (5′-CAGGUAA 3′). In some embodiments; the III domain comprises a nucleotide sequence, SEQ ID NO: 2305 (5′-CAGGUAAGUA-3′). Without wishing to be bound by theory, increasing the length of the U1 domain to include basepairing into stem 1 and/or basepairing to position 1 of U1 snRNA improves the U1 adaptor&#39;s affinity to Ul snRNA. 
     The annealing and effector domains of the Ul adaptor can be linked such that the effector domain is at the 5′ end and/or 3′ end of the annealing domain. The two domains can be linked by such that the 3′ end of one domain is linked to 5′ end of the other domain, or 3′ end of one domain is linked to 3′ end of the other domain, or 5′ end of one domain is linked to 5′ end of the other domain. The annealing and effector domains can be linked directly to each other or by a nucleotide based or non-nucleotide based linker. In some embodiments, a linker is nucleotide base and comprises 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, up to 15, up to 20, or up to 25 nucleotides. 
     In some embodiments, a linker between an annealing domain and an effector domain is multivalent, e.g., trivalent, tetravalent, pentavalent. Without wishing to be bound by theory, a multivalent linker can be used to link together a single annealing domain with a plurality of adaptor domains. 
     In some embodiments, a Ul adaptor comprises any oligonucleotide modification described herein. Exemplary such modifications include those that increase annealing affinity, specificity, bioavailability in the cell and organism, cellular and/or nuclear transport, stability, and/or resistance to degradation. In some embodiments, the U1 adaptor can be administered in combination with at least one other RNAi agent. 
     RNA Activators 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as RNA activators. 
     Recent studies have found that dsRNA can also activate gene expression, a mechanism that has been termed “small RNA-induced gene activation” or RNAa. See for example Li, L. C. et al.  Proc Nati Auld Sci USA . (2006), 103(46): 17337-42 and Li L. C. (2008). “Small RNAMediated Gene Activation”. RNA and the Regulation of Gene Expression: A Hidden Layer of Complexity. Caister Academic Press. ISBN 978-1-904455-25-7. It has been shown that dsRNAs targeting gene promoters induce potent transcriptional activation of associated genes. Endogenous miRNA that cause RNAa has also been found in humans. Check E. Nature (2007). 448 (7156): 855-858. 
     Another observation is that gene activation by RNAa is long-lasting. Induction of gene expression has been seen to last for over ten days. The prolonged effect of RNAa could be attributed to epigenetic changes at dsRNA target sites. 
     In some embodiments, a provided oligonucleotide is an RNA activator, wherein a provided oligonucleotide increases the expression of a gene. In some embodiments, increased gene expression inhibits viability, growth development, and/or reproduction. 
     Non-Coding RNAs (ncRNAs) 
     The present invention encompasses non-coding RNAs, such as long non-coding RNAs (lncRNAs) and short non-coding RNAs (sncRNAs). 
     Accordingly, provided oligonucleotide compositions of the present invention may be useful for modulating disease-associated long non-coding RNA. In some embodiments, oligonuclotides synthesized according to the methods provided herein are used to specifically block the binding of transcriptional repressor complexes to target lncRNA regions, thereby inducing the expression of the associated target gene. In some embodiments, the transcriptional repressor complex is a silencing factor. In some embodiments, the transcriptional repressor complex has Histone methyltransferase activity. In some embodiments, the transcriptional repressor complex methylates histone H3. In some embodiments, the transcriptional repressor complex is PRC2 (Polycomb Repressive Complex 2). 
     Certain PRC2-associated lncRNAs have been reported to be potential therapeutic targets and/or biomarkers (Zhao et al., 2010. “Genome-wide Identification of Polycomb-Associated RNAs by RIP-seq” Molecular Cell 40: 939-53). Overexpression of PCR2 proteins have been linked to various types of cancer, including metastatic prostate and breast cancer, and cancers of the colon, breast, and liver. Pharmacological inhibition of PRC2-mediated gene repression was found to induce apoptosis in several cancer cell lines in vitro, but not in various types of normal cells. Induction of apoptosis in this system is dependent on reactivation of genes that had been repressed by PRC2. There is also evidence that PRC2-mediated gene repression may be linked to the maintenance of the stem-cell properties of cancer stem cells. These results suggest that at least in some cases, inhibition of PRC2-mediated gene repression-including via targeting lncRNAs that recruit PRC2 to critical genes—is a potential strategy for treating various types of cancer. Non-limiting sequences of nucleic acids, which can be prepared in accordance with the methods provided herein, can be found in, for example, International Patent Publication WO 2012/065143 entitled “Polycomb-associated non-coding RNAs” (PCT/US2011/60493), the contents of which are incorporated by reference herein. 
     Provided oligonucleotide compositions of the present invention may be or act as small non-coding RNAs, such as piwi-interacting RNAs (piRNAs). The piRNAs represent the largest class of small non-coding RNA molecules that is expressed in animal cells and are found in clusters throughout the genome. piRNAs are known to form RNA-protein complexes through interactions with piwi proteins. piRNA complexes have been implicated in both epigenetic and post-transcriptional gene silencing of retrotransposons and other genetic elements in germ cells, including spermatogenesis. Typically piRNAs are 26-31 nucleotides in length, and, as compared to typical miRNAs, they lack sequence conservation and exhibit higher complexity. In some embodiments, oligonucleotide compositions utilized in accordance with the present invention comprise 5′ uridine. In some embodiments, oligonucleotide compositions utilized in accordance with the present invention comprise 5′ monophosphate and a 3′ modification that acts to block either the 2′ or 3′ oxygen. In some embodiments, the 3′ modification is a 2′-O-methylation. 
     Triplex Forming Oligonucleotides 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as triplex-forming oligonucleotides. 
     Recent studies have shown that triplex forming oligonucleotides (TFO) can be designed which can recognize and bind to polypurine/polypyrimidine regions in double-stranded helical DNA in a sequence-specific manner. These recognition rules are outline by Maher III, L. J., et al.,  Science  (1989) vol. 245, pp 725-730; Moser, H. E., et al.,  Science  (1987) vol. 238, pp 645-630; Beal, P. A., et al.,  Science  (1992) vol. 251, pp 1360-1363; Conney, M., et al.,  Science  (1988) vol. 241, pp 456-459 and Hogan, M. E., et al., EP Publication 375408. Modification of the oligonucleotides, such as the introduction of intercalators and intersugar linkage substitutions, and optimization of binding conditions (pH and cation concentration) have aided in. overcoming inherent obstacles to TFO activity such as charge repulsion and instability, and it was recently shown that oligonucleotides can be targeted to specific sequences (for a recent review sec Seidman and Glazer, Clin Invest 2003 2:487-94). In generaL the triplex-forming oligonucleotide has the sequence correspondence: 
     
       
         
           
               
               
            
               
                   
                 oligo 
               
               
                   
                 3′-A G G T 
               
               
                   
               
               
                   
                 duplex 
               
               
                   
                 5′-A G C T 
               
               
                   
               
               
                   
                 duplex 
               
               
                   
                 3′-T C G A 
               
            
           
         
       
     
     However, it has been shown that the A-AT and G-GC triplets have the greatest triple helical stability (Reither and Jeltsch, BMC Biochem, 2002, Sep. 12, Epub). The same authors have demonstrated that TFOs designed according to the A-AT and G-GC rule do not form nonspecific triplexes, indicating that the triplex formation is indeed sequence specific. Thus for any given sequence a triplex forming sequence can be devised. In some embodiments, triplex-forming oligonucleotides are at least about 15, about 25, or about 30 or more nucleotides in length. In some embodiments, triplex-forming oligonucleotides are up to about 50 or about 100 nucleotides in length. 
     Formation of the triple helical structure with the target DNA induces steric and functional changes, blocking transcription initiation and elongation, allowing the introduction of desired sequence changes in the endogenous DNA and resulting in the specific down-regulation of gene expression. Examples of such suppression of gene expression in cells treated with TFOs include knockout of episomal supFGI and endogenous HPRT genes in mammalian cells (Vasquez et al., Nucl Acids Res. 1999; 27: 1176-81, and Puri, et al, J Biol Chem, 2001; 276:28991-98), and the sequence- and target specific downregulation of expression of the Ets2 transcription factor, important in prostate cancer etiology (Carbone, et al, Nucl Acid Res. 2003; 31:833-43), and the pro-inflammatory ICAM-I gene (Besch et al, J Biol Chem, 2002; 277:32473-79). In addition, Vuyisich and Beal have recently shown that sequence specific TFOs can bind to dsRNA, inhibiting activity dsRNA-dependent enzymes such as RNA-dependent kinases (Vuyisich and Beal, Nuc, Acids Res 2000; 28:2369-74). 
     Additionally, TFO s designed according to the abovementioned principles can induce directed mutagenesis capable of effecting DNA repair, thus providing both down-regulation and up-regulation of expression of endogenous genes (Seidman and Glazer, J Invest 2003; 112:487-94). Detailed description of the design, synthesis and administration of effective TFOs can be found. S. Pat. App. Nos. 2003 017068 and 2003 0096980 to Froehier et at and 2002 012821/8 and 2002 0123476 to Emanude et aE, and U.S. Pat. No. 5,721,138 to Lawn, the contents of which are herein incorporated in their entireties. 
     Conjugates/Linkers 
     The invention also contemplates that utilized oligonucleotides, in some embodiments, are optimized for cellular uptake. In any utilized oligonucleotides embraced by the present invention, guide and/or passenger strands may be attached to a conjugate. In some embodiments the conjugate is hydrophobic. The hydrophobic conjugate can be a small molecule with a partition coefficient that is higher than 10. The conjugate can be a sterol-type molecule such as cholesterol, or a molecule with an increased length polycarbon chain attached to C17, and the presence of a conjugate can influence the ability of an RNA molecule to be taken into a cell with or without a lipid transfection reagent. The conjugate can be attached to the passenger or guide strand through a hydrophobic linker. In some embodiments, a hydrophobic linker is 5-12C in length, and/or is hydroxypyrrolidine-based. In some embodiments, a hydrophobic conjugate is attached to the passenger strand and the CU residues of either the passenger and/or guide strand are modified. In some embodiments, at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% or 95% of the CU residues on the passenger strand and/or the guide strand are modified. In some aspects, molecules associated with the invention are self-delivering (sd). As used herein, “self-delivery” refers to the ability of a molecule to be delivered into a cell without the need for an additional delivery vehicle such as a transfection reagent. Aspects of the invention relate to selecting molecules for use in RNAi. 
     In any of the embodiments embraced herein, utilized oligonucleotides may be associated with a hydrophobic moiety for targeting and/or delivery of the molecule to a cell. In some embodiments, the hydrophobic moiety is associated with the nucleic acid molecule through a linker. In some embodiments, the association is through non-covalent interactions. In other some, the association is through a covalent bond. Any linker known in the art may be used to associate the nucleic acid with the hydrophobic moiety. Linkers known in the art are described in published international PCT applications, WO 92/03464, WO 95/23162, WO 2008/021157, WO 2009/021157, WO 2009/134487, WO 2009/126933, U.S. Patent Application Publication 2005/0107325, U.S. Pat. Nos. 5,414,077, 5,419,966, 5,512,667, 5,646,126, and 5,652,359, which are incorporated herein by reference. The linker may be as simple as a covalent bond to a multi-atom linker. The linker may be cyclic or acyclic. The linker may be optionally substituted. In some embodiments, the linker is capable of being cleaved from the nucleic acid. In certain embodiments, the linker is capable of being hydrolyzed under physiological conditions. In some embodiments, the linker is capable of being cleaved by an enzyme (e.g., an esterase or phosphodiesterase). In some embodiments, the linker comprises a spacer element to separate the nucleic acid from the hydrophobic moiety. The spacer element may include one to thirty carbon or heteroatoms. In certain embodiments, the linker and/or spacer element comprises protonatable functional groups. Such protonatable functional groups may promote the endosomal escape of the nucleic acid molecule. The protonatable functional groups may also aid in the delivery of the nucleic acid to a cell, for example, neutralizing the overall charge of the molecule. In some embodiments, the linker and/or spacer element is biologically inert (that is, it does not impart biological activity or function to the resulting nucleic acid molecule). 
     The hydrophobic molecule may be connected to the polynucleotide by a linker moiety. Optionally the linker moiety is a non-nucleotidic linker moiety. Non-nucleotidic linkers are e.g. abasic residues (dSpacer), oligoethyleneglycol, such as triethyleneglycol (spacer 9) or hexaethyleneglycol (spacer 18), or alkane-diol, such as butanediol. The spacer units are preferably linked by phosphodiester or phosphorothioate bonds. The linker units may appear just once in the molecule or may be incorporated several times, e.g. via phosphodiester, phosphorothioate, methylphosphonate, or amide linkages. 
     Typical conjugation protocols involve the synthesis of polynucleotides bearing an aminolinker at one or more positions of the sequence, however, a linker is not required. The amino group is then reacted with the molecule being conjugated using appropriate coupling or activating reagents. The conjugation reaction may be performed either with the polynucleotide still bound to a solid support or following cleavage of the polynucleotide in solution phase. Purification of the modified polynucleotide by HPLC typically results in a pure material. 
     In some embodiments, a linking group can be attached to a nucleomonomer and the transporting peptide can be covalently attached to the linker. In some embodiments, a linker can function as both an attachment site for a transporting peptide and can provide stability against nucleases. Examples of suitable linkers include substituted or unsubstituted C 1 -C 20  alkyl chains, C 2 -C 20  alkenyl chains, C 2 -C 20  alkynyl chains, peptides, and heteroatoms (e.g., S, O, NH, etc.). Other exemplary linkers include bifunctional crosslinking agents such as sulfosuccinimidyl-4-(maleimidophenyl)-butyrate (SMPB) (see, e.g., Smith et al. Biochem J 1991.276: 417-2). 
     In some embodiments, provided oligonucleotides of the invention are synthesized as molecular conjugates which utilize receptor-mediated endocytotic mechanisms for delivering genes into cells (see, e.g., Bunnell et al. 1992. Somatic Cell and Molecular Genetics. 18:559, and the references cited therein). 
     Targeting Agents 
     In some embodiments, provided compositions include one or more targeting agents, associated with the composition and/or with individual oligonucleotides therein. Exemplary such targeting agents are described above and further herein. The phrases “targeting agent” and “targeting moiety” are used herein interchangeably. 
     Delivery of oligonucleotides and/or compositions thereof can often be improved by targeting the oligonucleotides to a cellular receptor. The targeting moieties can be conjugated to the oligonucleotides or attached to a carrier group (i.e., poly(L-lysine) or liposomes) linked to the oligonucleotides. This method is well suited to cells that display specific receptor-mediated endocytosis. 
     For instance, oligonucleotide conjugates to 6-phosphomannosylated proteins are internalized 20-fold more efficiently by cells expressing mannose 6-phosphate specific receptors than free oligonucleotides. The oligonucleotides may also be coupled to a ligand for a cellular receptor using a biodegradable linker. In another example, the delivery construct is mannosylated streptavidin which forms a tight complex with biotinylated oligonucleotides. Mannosylated streptavidin was found to increase 20-fold the internalization of biotinylated oligonucleotides. (Vlassov et al. 1994. Biochimica et Biophysica Acta 1197:95-108). 
     The field of RNA interference has revolutionized the study of biological processes. These tools include small inhibitory RNA (siRNA), small hairpin RNA (shRNA) and ribozymes. As RNA interference technology has grown, so has the list of validated RNA target sequences and reagents for use. Provided compositions and methods embraced by the present application can be readily applied to each of such target sequences. For a database of validated siRNA target sequences and links to kits and reagents that may be used for RNA interference experiments, see, for example, http://www.rnainterference.org/index.html. Exemplary siRNA target sequences useful for the present invention are provided in the accompanying Appendix (A). 
     Immunomodulatory Oligonucleotides 
     In some embodiments, provided compositions include one or more oligonucleotides that are or act as immunomodulatory oligonucleotides. 
     Oligonucleotides utilized in accordance with the invention can be immunomodulatory agents, i.e., agents that are capable of modulating or regulating an immune response when administered to a subject. Immune responses elicited by such an agent can be an innate and/or an adaptive immune response. The immune system is divided into a more innate immune system, and acquired adaptive immune system of vertebrates, the latter of which is further divided into humoral cellular components. In some embodiments, the immune response can be mucosal. Immunomodulatory motifs described herein can be used in the context of previously described classes of immunomodulatory oligonucleotides including ODN classes such as A class, B class, C class, E class, T class and P class. 
     In some embodiments of the invention immunomodulatory oligonucleotides utilized in accordance with the present invention include one or more immunostimulatory motifs. In some embodiments, oligonucleotides utilized in accordance with the present invention include one or more “CpG dinucleotides.” A CpG dinucleotide can be methylated or unmethylated. An immunostimulatory oligonucleotide containing at least one unmethylated CpG dinucleotide is an oligonucleotide molecule which contains an unmethylated cytosine-guanine dinucleotide sequence (i.e., an unmethylated 5′ cytidine followed by 3′ guanosine and linked by a phosphate bond) and which activates the immune system; such an immunostimulatory oligonucleotide is a CpG oligonucleotide. CpG oligonucleotides have been described in a number of issued patents, published patent applications, and other publications, including: U.S. Pat. Nos. 6,194,388; 6,207,646; 6,214,806; 6,218,371; 6,239,116; and 6,339,068, the contents of which are incorporated herein by reference. In some embodiments, utilized oligonucleotides are or act as an agonist for a Toll-like receptor. In some embodiments, utilized oligonucleotides are or act as an agonist for Toll-like receptor 9 (TLR9). In some embodiments, utilized oligonucleotides are or act as an antagonist for a Toll-like receptor. In some embodiments, utilized oligonucleotides are or act as an antagonist for Toll-like receptor 9 (TLR9). In some embodiments, stereo-defined oligonucleotides embraced by the invention exhibit greater affinity for respective receptor/target, as compared to stereo-random counterpart. In some embodiments, stereo-defined oligonucleotides embraced by the invention elicit one or more immune responses, when administered to subjects that meet certain clinical criteria, with less degree of variables amongst the population, as compared to stereo-random counterpart. In some embodiments, stereo-defined oligonucleotides embraced by the invention cause less degree of toxic side effects or fewer side effects, when administered to subjects that meet certain clinical criteria, as compared to stereo-random counterpart. 
     In some embodiments, immunostimulatory oligonucleotides utilized in accordance with the present invention are free of CpG dinucleotide motifs. These oligonucleotides which are free of CpG dinucleotides are referred to as non-CpG oligonucleotides, and they have non-CpG immunostimulatory motifs. In some embodiments, these are T-rich immunostimulatory oligonucleotides, such as oligonucleotides having at least 80% T. 
     In some embodiments, immunostimulatory oligonucleotides utilized in accordance with the present invention are B class immunomodulatory oligonucleotides. 
     “B class” ODN are potent at activating B cells but are relatively weak in inducing IFN-α and NK cell activation. The B class CpG oligonucleotides typically are fully stabilized and include an unmethylated CpG dinucleotide within certain preferred base contexts. See, e.g., U.S. Pat. Nos. 6,194,388; 6,207,646; 6,214,806; 6,218,371; 6,239,116; and 6,339,068. 
     Another class is potent for inducing IFN-α and NK cell activation but is relatively weak at stimulating B cells; this class has been termed the “A class”. In some embodiments, immunostimulatory oligonucleotides utilized in accordance with the present invention are A class immunomodulatory oligonucleotides. The A class CpG oligonucleotides typically have stabilized poly-G sequences at 5′ and 3′ ends and a palindromic phosphodiester CpG dinucleotide-containing sequence of at least 6 nucleotides. See, for example, published patent application PCT/US00/26527 (WO 01/22990). 
     Yet another class of CpG oligonucleotides activates B cells and NK cells and induces IFN-α; this class has been termed the C-class. In some embodiments, immunostimulatory oligonucleotides utilized in accordance with the present invention are C class immunomodulatory oligonucleotides. The “C class” immunostimulatory oligonucleotides contain at least two distinct motifs have unique and desirable stimulatory effects on cells of the immune system. Some of these ODN have both a traditional “stimulatory” CpG sequence and a “GC-rich” or “B-cell neutralizing” motif. These combination motif oligonucleotides have immune stimulating effects that fall somewhere between those effects associated with traditional “class B” CpG ODN, which are strong inducers of B cell activation and dendritic cell (DC) activation, and those effects associated with a more recently described class of immune stimulatory oligonucleotides (“class A” CpG ODN) which are strong inducers of IFN-α and natural killer (NK) cell activation but relatively poor inducers of B-cell and DC activation. Krieg A M et al. (1995) Nature 374:546-9; Ballas Z K et al. (1996) J Immunol 157:1840-5; Yamamoto S et al. (1992) J Immunol 48:4072-6. While preferred class B CpG ODN often have phosphorothioate backbones and preferred class A CpG ODN have mixed or chimeric backbones, the C class of combination motif immune stimulatory oligonucleotides may have either stabilized, e.g., phosphorothioate, chimeric, or phosphodiester backbones, and in some preferred embodiments, they have semi-soft backbones. This class has been described in U.S. patent application Ser. No. 10/224,523 filed on Aug. 19, 2002, the entire contents of which is incorporated herein by reference. 
     In some embodiments, immunostimulatory oligonucleotides utilized in accordance with the present invention are E class immunomodulatory oligonucleotides. The “E class” oligonucleotides have an enhanced ability to induce secretion of IFN-alpha. These ODN have a lipophilic substituted nucleotide analog 5′ and/or 3′ of a YGZ motif. The compound of the E class formula may be, for example, any of the following lipophilic substituted nucleotide analogs: a substituted pyrimidine, a substituted uracil, a hydrophobic T analog, a substituted toluene, a substituted imidazole or pyrazole, a substituted triazole, 5-chloro-uracil, 5-bromouracil, 5-iodo-uracil, 5-ethyl-uracil, 5-propyl-uracil, 5-propinyl-uracil, (E)-5-(2-bromovinyl)-uracil, or 2,4-difluoro-toluene. E class oligonucleotides are described at least in provisional patent application U.S. 60/847,811. 
     In some embodiments, immunostimulatory oligonucleotides utilized in accordance with the present invention are T class immunomodulatory oligonucleotides. The “T class” oligonucleotides induce secretion of lower levels of IFN-alpha when not modified as in the ODNs of the invention and IFN-related cytokines and chemokines than B class or C class oligonucleotides, while retaining the ability to induce levels of IL-10 similar to B class oligonucleotides. T class oligonucleotides are described at least in U.S. patent application Ser. No. 11/099,683, the entire contents of which are hereby incorporated by reference. 
     In some embodiments, immunostimulatory oligonucleotides utilized in accordance with the present invention are P class immunomodulatory oligonucleotides. The “P class” immunostimulatory oligonucleotides have several domains, including a 5TLR activation domain, 2 duplex forming regions and an optional spacer and 3′ tail. This class of oligonucleotides has the ability in some instances to induce much higher levels of IFN-α secretion than the C-Class. The P-Class oligonucleotides have the ability to spontaneously self-assemble into concatamers either in vitro and/or in vivo. Without being bound by any particular theory for the method of action of these molecules, one potential hypothesis is that this property endows the P-Class oligonucleotides with the ability to more highly crosslink TLR9 inside certain immune cells, inducing a distinct pattern of immune activation compared to the previously described classes of CpG oligonucleotides. Cross-linking of TLR9 receptors may induce activation of stronger IFN-α secretion through the type I IFNR feedback loop in plasmacytoid dendritic cells. P class oligonucleotides are described at least in U.S. application Ser. No. 11/706,561. 
     In some embodiments, immunostimulatory oligonucleotides utilized in accordance with the present invention are S class immunomodulatory oligonucleotides. The immunomodulatory oligonucleotides of the instant invention may be immunosuppressive oligonucleotides. The immunomodulatory motifs described above can be used in the context of previously described classes of immunosuppressive oligonucleotides including ODN classes such as the “S class”. Inhibitory, or S class, ODN are useful whenever it is desirable to inhibit immunostimulation. Inhibitory ODN can be used for preventing and treating septic shock, inflammation, allergy, asthma, graft rejection, graft-versus host disease (GvHD), autoimmune diseases, Th1- or Th2-mediated diseases, bacterial infections, parasitic infections, spontaneous abortions, and tumors. The inhibitory ODN can be used generally to inhibit activation of all cells expressing the relevant TLRs, and more specifically to inhibit activation of antigen-presenting cells, B cells, plasmacytoid dendritic cells (pDCs), monocytes, monocyte-derived cells, eosinophils, and neutrophils. S class ODN are further described at least in U.S. application Ser. No. 10/977,560. 
     According to the invention, immunomodulatory oligonucleotides may have a backbone of stabilized internucleotide linkages in addition to the stabilizing FANA purine nucleotide(s) or have a chimeric backbone of stabilized and phosphodiester nucleotide linkages. A “stabilized internucleotide linkage” shall mean an internucleotide linkage that is relatively resistant to in vivo degradation (e.g., via an exo- or endo-nuclease), compared to a phosphodiester internucleotide linkage. In some embodiments, stabilized internucleotide linkages include, without limitation, phosphorothioate, phosphorodithioate, methylphosphonate, methylphosphorothioate, phosphonoacetate, Rp-phosphorothioate, Sp-phosphorothioate, boranophosphate, or 3′-thioformacetal, or combinations thereof. Other stabilized oligonucleotides include: nonionic DNA analogs, such as alkyl- and aryl-phosphates (in which the charged phosphonate oxygen is replaced by an alkyl or aryl group), phosphodiester and alkylphosphotriesters, in which the charged oxygen moiety is alkylated. Oligonucleotides which contain diol, such as tetraethyleneglycol or hexaethyleneglycol, at either or both termini have also been shown to be substantially resistant to nuclease degradation. 
     As described in more detained herein, according to the invention, these and other modifications can be selectively introduced at a predetermined position(s)/pattern(s) within an oligonucleotide so as to obtain a stereospecific oligonucleotide molecule. Furthermore, according to the invention, a composition comprising a plurality of such oligonucleotides at an extremely high degree of purity (e.g., at least 96%, at least 97%, at least 98%, at least 99%, essentially 100%) can be obtained. Thus, provided oligonucleotide compositions comprising oligonucleotides of one or more predetermined types are suitable for in vivo administration. It is contemplated that, due to high degree of structural purity of oligonucleotides in a composition (such that a composition comprises a single specified type of oligonucleotides), provided compositions can elicit improved biological activities, increased efficacy, reduced variables in response, and/or reduced unwanted side effects, when administered to subjects. 
     Accordingly, utilized oligonucleotides of the invention are particularly useful as an therapeutic agent when formulated into a pharmaceutical composition. In some embodiments, such therapeutic agent is an immunomodulatory agent. In some embodiments, provided immunomodulatory agents are immunostimulatory agents. In some embodiments, provided immunomodulatory agents are immunoinhibitory (or immunosuppressive) agents. In some embodiments, provided immunomodulatory agents act as an adjuvant. In some embodiments, provided immunomodulatory agents can shift a Th2 type immune response to a Th1 type immune response by inducing Th1 type cytokines that can suppress inflammatory Th2 response. In some embodiments, provided oligonucleotide compositions are useful as an agent that regulates gene expression. For example, in some embodiments, provided oligonucleotide compositions are useful as an agent capable of silencing a gene of interest, e.g., genes associated with disease or disorder. In some embodiments, provided oligonucleotide compositions are useful as an agent to regulate RNA splicing. 
     For example, some embodiments of such oligonucleotides include those comprising at least one CpG dinucleotide motif. In some embodiments, such oligonucleotides comprise at least one unmethylated CpG dinucleotide motif. In some embodiments, CpG-containing oligonucleotides useful for the present invention are categorized as Class A (type D) oligonucleotides. In some embodiments, CpG-containing oligonucleotides useful for the present invention are categorized as Class B (type K) oligonucleotides. In some embodiments, CpG-containing oligonucleotides useful for the present invention are categorized as Class C oligonucleotides. In some embodiments, CpG-containing oligonucleotides useful for the present invention are categorized as Class P oligonucleotides. In some embodiments, CpG-containing oligonucleotides useful for the present invention contain at least one palindromic sequence. In some embodiments, CpG-containing oligonucleotides useful for the present invention can form a “dumbbell-like” structure. In some embodiments, CpG-containing oligonucleotides useful for the present invention can form a “Y” shaped structure, or multimers thereof. In some embodiments, CpG-containing oligonucleotides useful for the present invention can form a tetrahedral structure. For reviews, see, for example: Bode et al. (2011) Expert Rev. Vaccines 10(4): 499-511; Hanagata (2012) Int. J. Nanomedicine 7:2181-95. 
     In some embodiments, oligonucleotides utilized in accordance with the present invention elicit immunomodulatory effects on cells expressing Toll-like receptor(s). In some embodiments, target cells that respond to such oligonucleotides include, but are not limited to, antigen-presenting cells (APCs), antigen-specific T and B cells, cytotoxic T lymphocytes (CTLs), natural killer (NK) cells, and dendritic cells (DCs). In some embodiments, immunomodulatory effects are the direct effects elicited by cells expressing a receptor or receptors that recognize such an oligonucleotide. In some embodiments, such oligonucleotides elicit immunomodulatory effects on cells expressing Toll-like receptor 9 (TLR9) as ligands. For example, some embodiments of the invention include CpG oligonucleotides that act as agonists of one or more Toll-like receptors. Some embodiments of the invention include CpG oligonucleotides that act as antagonists of one or more Toll-like receptors. In some embodiments, immunomodulatory effects are indirect effects that occur downstream, involving cells that do not necessarily respond directly to such oligonucleotides or express such receptors. 
     In some embodiments, CpG oligonucleotides useful for the present invention are characterized in that they directly activate human B cells and plasmacytoid dendritic cells via TLR-9. In some embodiments, CpG oligodeoxynucleotides useful for the present invention are characterized in that indirectly support the maturation and proliferation of natural killer cells, T cells and monocytes/macrophages. 
     In some embodiments, CpG oligonucleotides useful for the present invention trigger an immune response, which is characterized by the production of Th1-type and proinflammatory cytokines, chemokines and polyreactive IgM. 
     In some embodiments, CpG oligonucleotides useful for the present invention are characterized in that the immunogenicity of conventional protein antigens and peptide-based vaccines is enhanced by such oligonucleotides. Without wising to be bound by a particular theory, it is believed that such adjuvant effect is mediated through improved function of professional antigen-presenting cells and the resultant generation of humoral and cellular vaccine-specific immune responses. 
     In some embodiments, CpG oligonucleotides useful for the present invention are characterized in that CpG oligonucleotides increase the magnitude and accelerate the development of vaccineinduced responses. They also improve the induction of memory, thereby extending the duration of humoral and cellular immunity. 
     In some embodiments, CpG oligonucleotides useful for the present invention are characterized in that they boost immunity in groups of subjects (e.g., populations of patients) with reduced immune function, such as the elderly and those with suppressed immune system. They are effective when administered either systemically or mucosally. Preclinical and clinical trials using CpG oligonucleotides of mixed chirality (e.g., not chirally pure) demonstrate CpG oligonucleotides can boost the immunogenicity of vaccines targeting infectious diseases and cancer. Furthermore, clinical trials indicate that CpG oligonucleotides are reasonably safe when administered s vaccine adjuvants. 
     Immunomodulatory oligonucleotides prepared in accordance with the present disclosure are useful for a number of therapeutic applications. Therefore, utilized immunomodulatory oligonucleotides may be formulated into suitable pharmaceutical compositions. Such pharmaceutical compositions can be administered to a subject in an amount effective to treat a disease, disorder or condition, as described in further detail herein by suitable routes of administration. According to the invention, an effective amount of an immunostimulatory oligonucleotides may be administered to a subject who is likely to benefit from boosting the immune system (e.g., enhanced immune response). In some embodiments, clinical benefits are conferred directly by an immunomodulatory oligonucleotide acting upon its target immune cells, e.g., via binding of the ligand to its Toll-like receptor proteins. In some embodiments, clinical benefits are achieved at least in part indirectly by overall boosting of the immune system of the subject. 
     As used herein, the terms “effective amount” and “effective dose” refer to any amount or dose of a compound or composition that is sufficient to fulfill its intended purpose(s), i.e., a desired biological or medicinal response in a tissue or subject at an acceptable benefit/risk ratio. The relevant intended purpose may be objective (i.e., measurable by some test or marker) or subjective (i.e., subject gives an indication of or feels an effect). In some embodiments, a therapeutically effective amount is an amount that, when administered to a population of subjects that meet certain clinical criteria for a disease or disorder (for example, as determined by symptoms manifested, disease progression/stage, genetic profile, etc.), a statistically significant therapeutic response is obtained among the population. A therapeutically effective amount is commonly administered in a dosing regimen that may comprise multiple unit doses. For any particular pharmaceutical agent, a therapeutically effective amount (and/or an appropriate unit dose within an effective dosing regimen) may vary, for example, depending on route of administration, on combination with other pharmaceutical agents. In some embodiments, the specific therapeutically effective amount (and/or unit dose) for any particular patient may depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific pharmaceutical agent employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and/or rate of excretion or metabolism of the specific pharmaceutical agent employed; the duration of the treatment; and like factors as is well known in the medical arts. Those of ordinary skill in the art will appreciate that in some embodiments of the invention, a unit dosage may be considered to contain an effective amount if it contains an amount appropriate for administration in the context of a dosage regimen correlated with a positive outcome. In some embodiments, CpG oligodeoxynucleotides prepared according to the present invention therefore may exert improved efficacy and immunomodulatory effects due to their chiral purity. In some embodiments, an effective amount of provided immunomodulatory oligonucleotides is less than that of less-pure counterpart. 
     In some embodiments, an immunomodulatory oligonucleotide composition is administered to a healthy subject. In some embodiments, an immunostimulatory oligonucleotide composition is administered to a subject as part of a vaccine, in which the immunostimulatory oligonucleotide acts as an adjuvant. In some embodiments, an immunostimulatory oligonucleotide composition is administered to a subject with a suppressed (e.g., compromised) immune system. In some embodiments, a subject, whose immune system is suppressed and does not sufficiently respond to a conventional vaccine, responds to a vaccine comprising an immunostimulatory oligonucleotide embraced by the present invention. In some embodiments, a subject who may benefit from such a vaccine has a suppressed immune system associated with an infection, such as viral infection, e.g., HIV, HBV, HCV, etc. 
     The invention encompasses the use of immunomodulatory oligonucleotides contemplated herein for treatment of a subject having a condition that may be treated by stimulation or suppression of the immune response. Thus, provided immunomodulatory oligonucleotide compositions are useful for the treatment of diseases or conditions, including, without limitation, infection, cancer, allergy, asthma, an inflammatory condition, an autoimmune disease, and any combination thereof. 
     In some embodiments, provided immunomodulatory oligonucleotide compositions are useful in some aspects of the invention for the treatment of a subject at risk of developing a clinical condition. Non-limiting clinical conditions include allergy, asthma, an infection with an infectious organism, cancer, inflammation, and autoimmune disease. A subject at risk as used herein is a subject who has any risk of exposure to an infection causing pathogen or a cancer or an allergen or a risk of developing cancer. For instance, a subject at risk may be a subject who is planning to travel to an area where a particular type of infectious agent is found, or it may be a subject who through lifestyle or medical procedures is exposed to bodily fluids which may contain infectious organisms or directly to the organism, or even any subject living in an area where an infectious organism or an allergen has been identified. Subjects at risk of developing infection also include general populations to which a medical agency recommends vaccination with a particular infectious organism antigen. If the antigen is an allergen and the subject develops allergic responses to that particular antigen and the subject may be exposed to the antigen, i.e., during pollen season, then that subject is at risk of exposure to the antigen. A subject at risk of developing allergy or asthma includes those subjects that have been identified as having an allergy or asthma but that don&#39;t have the active disease during the immunomodulatory oligonucleotide treatment as well as subjects that are considered to be at risk of developing these diseases because of genetic or environmental factors. A subject at risk of developing a cancer is one who has a high probability of developing cancer. These subjects include, for instance, subjects having a genetic abnormality, the presence of which has been demonstrated to have a correlative relation to a higher likelihood of developing a cancer and subjects exposed to cancer causing agents such as tobacco, asbestos, or other chemical toxins, or a subject who has previously been treated for cancer and is in apparent remission. When a subject at risk of developing a cancer is treated with an antigen specific for the type of cancer to which the subject is at risk of developing and a CpG immunostimulatory oligonucleotide, the subject may be able to kill the cancer cells as they develop. If a tumor begins to form in the subject, the subject will develop a specific immune response against the tumor antigen. 
     In some embodiments, provided immunomodulatory oligonucleotide compositions are useful for treating a subject having an immune disease or disorder, including asthma, allergy, and related conditions. 
     A subject having an allergy is a subject that has or is at risk of developing an allergic reaction in response to an allergen. An allergy refers to acquired hypersensitivity to a substance (allergen). Allergic conditions include but are not limited to eczema, allergic rhinitis or coryza, hay fever, conjunctivitis, bronchial asthma, urticaria (hives) and food allergies, and other atopic conditions. 
     Allergies are generally caused by IgE antibody generation against harmless allergens. The cytokines that are induced by systemic or mucosal administration of immunomodulatory oligonucleotides are predominantly of a class called Th1 (examples are IL-12, IP-10, IFN-α and IFN-γ) and these induce both humoral and cellular immune responses. The other major type of immune response, which is associated with the production of IL-4 and IL-5 cytokines, is termed a Th2 immune response. In general, it appears that allergic diseases are mediated by Th2 type immune responses. Based on the ability of the immunomodulatory oligonucleotide to shift the immune response in a subject from a predominant Th2 (which is associated with production of IgE antibodies and allergy) to a balanced Th2/Th1 response (which is protective against allergic reactions), an effective dose for inducing an immune response of a immunomodulatory oligonucleotide can be administered to a subject to treat or prevent asthma and allergy. 
     Thus, provided immunomodulatory oligonucleotide compositions have significant therapeutic utility in the treatment of allergic and non-allergic conditions such as asthma. Th2 cytokines, especially IL-4 and IL-5 are elevated in the airways of asthmatic subjects. These cytokines promote important aspects of the asthmatic inflammatory response, including IgE isotope switching, eosinophil chemotaxis and activation and mast cell growth. Th1 cytokines, especially IFN-γ and IL-12, can suppress the formation of Th2 clones and production of Th2 cytokines. Asthma refers to a disorder of the respiratory system characterized by inflammation, narrowing of the airways and increased reactivity of the airways to inhaled agents. Asthma is frequently, although not exclusively associated with atopic or allergic symptoms. 
     Provided immunomodulatory oligonucleotide compositions may also be administered in conjunction with an anti-allergy therapy. Conventional methods for treating or preventing allergy have involved the use of allergy medicaments or desensitization therapies. Some evolving therapies for treating or preventing allergy include the use of neutralizing anti-lgE antibodies. Anti-histamines and other drugs which block the effects of chemical mediators of the allergic reaction help to regulate the severity of the allergic symptoms but do not prevent the allergic reaction and have no effect on subsequent allergic responses. Desensitization therapies are performed by giving small doses of an allergen, usually by injection under the skin, in order to induce an IgG-type response against the allergen. The presence of IgG antibody helps to neutralize the production of mediators resulting from the induction of IgE antibodies, it is believed. Initially, the subject is treated with a very low dose of the allergen to avoid inducing a severe reaction and the dose is slowly increased. This type of therapy is dangerous because the subject is actually administered the compounds which cause the allergic response and severe allergic reactions can result. 
     Anti-allergy medicaments include, but are not limited to, anti-histamines, corticosteroids, and prostaglandin inducers. Anti-histamines are compounds which counteract histamine released by mast cells or basophils. These compounds are well known in the art and commonly used for the treatment of allergy. Anti-histamines include, but are not limited to, acrivastine, astemizole, azatadine, azelastine, betatastine, brompheniramine, buclizine, cetirizine, cetirizine analogues, chlorpheniramine, clemastine, CS 560, cyproheptadine, desloratadine, dexchlorpheniramine, ebastine, epinastine, fexofenadine, HSR 609, hydroxyzine, levocabastine, loratidine, methscopolamine, mizolastine, norastemizole, phenindamine, promethazine, pyrilamine, terfenadine, and tranilast. Corticosteroids include, but are not limited to, methylprednisolone, prednisolone, prednisone, beclomethasone, budesonide, dexamethasone, flunisolide, fluticasone propionate, and triamcinolone. Although dexamethasone is a corticosteroid having antiinflammatory action, it is not regularly used for the treatment of allergy or asthma in an inhaled form because it is highly absorbed and it has long-term suppressive side effects at an effective dose. Dexamethasone, however, can be used according to the invention for treating allergy or asthma because when administered in combination with a composition of the invention it can be administered at a low dose to reduce the side effects. Some of the side effects associated with corticosteroid use include cough, dysphonia, oral thrush (candidiasis), and in higher doses, systemic effects, such as adrenal suppression, glucose intolerance, osteoporosis, aseptic necrosis of bone, cataract formation, growth suppression, hypertension, muscle weakness, skin thinning, and easy bruising. Barnes &amp; Peterson (1993) Am Rev Respir Dis 148:S1-S26; and Kamada A K et al. (1996) Am J Respir Crit Care Med 153:1739-48. 
     Provided oligonucleotide compositions and methods in accordance with the invention can be used alone or in conjunction with other agents and methods useful for the treatment of asthma. In one aspect the invention provides a method of treating a subject having asthma. The method according to this aspect of the invention includes the step of administering to a subject having asthma an effective amount of a composition of the invention to treat the subject. 
     In some embodiments, the invention provides a method of treating a subject having asthma. The method according to this aspect of the invention includes the step of administering to a subject having asthma an effective amount of the composition of the invention and an anti-asthma therapy to treat the subject. 
     “Asthma” as used herein refers to a disorder of the respiratory system characterized by inflammation and narrowing of the airways, and increased reactivity of the airways to inhaled agents. Asthma is frequently, although not exclusively, associated with an atopic or allergic condition. Symptoms of asthma include recurrent episodes of wheezing, breathlessness, chest tightness, and coughing, resulting from airflow obstruction. Airway inflammation associated with asthma can be detected through observation of a number of physiological changes, such as, denudation of airway epithelium, collagen deposition beneath basement membrane, edema, mast cell activation, inflammatory cell infiltration, including neutrophils, eosinophils, and lymphocytes. As a result of the airway inflammation, asthma patients often experience airway hyper-responsiveness, airflow limitation, respiratory symptoms, and disease chronicity. Airflow limitations include acute bronchoconstriction, airway edema, mucous plug formation, and airway remodeling, features which often lead to bronchial obstruction. In some cases of asthma, sub-basement membrane fibrosis may occur, leading to persistent abnormalities in lung function. 
     Research over the past several years has revealed that asthma likely results from complex interactions among inflammatory cells, mediators, and other cells and tissues resident in the airways. Mast cells, eosinophils, epithelial cells, macrophage, and activated T cells all play an important role in the inflammatory process associated with asthma. Djukanovic R et al. (1990) Am Rev Respir Dis 142:434-457. It is believed that these cells can influence airway function through secretion of preformed and newly synthesized mediators which can act directly or indirectly on the local tissue. It has also been recognized that subpopulations of T lymphocytes (Th2) play an important role in regulating allergic inflammation in the airway by releasing selective cytokines and establishing disease chronicity. Robinson D S et al. (1992) N Engl J Med 326:298-304. 
     Asthma is a complex disorder which arises at different stages in development and can be classified based on the degree of symptoms as acute, subacute, or chronic. An acute inflammatory response is associated with an early recruitment of cells into the airway. The subacute inflammatory response involves the recruitment of cells as well as the activation of resident cells causing a more persistent pattern of inflammation. Chronic inflammatory response is characterized by a persistent level of cell damage and an ongoing repair process, which may result in permanent abnormalities in the airway. 
     A “subject having asthma” is a subject that has a disorder of the respiratory system characterized by inflammation and narrowing of the airways and increased reactivity of the airways to inhaled agents. Factors associated with initiation of asthma include, but are not limited to, allergens, cold temperature, exercise, viral infections, and SO 2 . 
     As mentioned above, asthma may be associated with a Th2-type of immune response, which is characterized at least in part by Th2 cytokines IL-4 and IL-5, as well as antibody isotype switching to IgE. Th1 and Th2 immune responses are mutually counter-regulatory, so that skewing of the immune response toward a Th1-type of immune response can prevent or ameliorate a Th2-type of immune response, including allergy. Provided immunomodulatory oligonucleotide compositions of the invention are therefore useful by themselves to treat a subject having asthma because the analogs can skew the immune response toward a Th1-type of immune response. 
     Provided immunomodulatory oligonucleotide compositions of the invention may also be administered in conjunction with an asthma therapy. Conventional methods for treating or preventing asthma have involved the use of anti-allergy therapies (described above) and a number of other agents, including inhaled agents. 
     Medications for the treatment of asthma are generally separated into two categories, quick-relief medications and long-term control medications. Asthma patients take the long-term control medications on a daily basis to achieve and maintain control of persistent asthma. Long-term control medications include anti-inflammatory agents such as corticosteroids, chromolyn sodium and nedocromil; long-acting bronchodilators, such as long-acting β 2 -agonists and methylxanthines; and leukotriene modifiers. The quick-relief medications include short-acting β 2  agonists, anticholinergics, and systemic corticosteroids. There are many side effects associated with each of these drugs and none of the drugs alone or in combination is capable of preventing or completely treating asthma. 
     Anti-asthma medicaments include, but are not limited to, PDE-4 inhibitors, bronchodilator/beta-2 agonists, K+ channel openers, VLA-4 antagonists, neurokin antagonists, thromboxane A2 (TXA2) synthesis inhibitors, xanthines, arachidonic acid antagonists, 5 lipoxygenase inhibitors, TXA2 receptor antagonists, TXA2 antagonists, inhibitor of 5-lipox activation proteins, and protease inhibitors. 
     Bronchodilator/β 2  agonists are a class of compounds which cause bronchodilation or smooth muscle relaxation. Bronchodilator/β 2  agonists include, but are not limited to, salmeterol, salbutamol, albuterol, terbutaline, D2522/formoterol, fenoterol, bitolterol, pirbuerol methylxanthines and orciprenaline. Long-acting β 2  agonists and bronchodilators are compounds which are used for long-term prevention of symptoms in addition to the anti-inflammatory therapies. Long-acting β 2  agonists include, but are not limited to, salmeterol and albuterol. These compounds are usually used in combination with corticosteroids and generally are not used without any inflammatory therapy. They have been associated with side effects such as tachycardia, skeletal muscle tremor, hypokalemia, and prolongation of QTc interval in overdose. 
     Methylxanthines, including for instance theophylline, have been used for long-term control and prevention of symptoms. These compounds cause bronchodilation resulting from phosphodiesterase inhibition and likely adenosine antagonism. Dose-related acute toxicities are a particular problem with these types of compounds. As a result, routine serum concentration must be monitored in order to account for the toxicity and narrow therapeutic range arising from individual differences in metabolic clearance. Side effects include tachycardia, tachyarrhythmias, nausea and vomiting, central nervous system stimulation, headache, seizures, hematemesis, hyperglycemia and hypokalemia. Short-acting β 2  agonists include, but are not limited to, albuterol, bitolterol, pirbuterol, and terbutaline. Some of the adverse effects associated with the administration of short-acting β 2  agonists include tachycardia, skeletal muscle tremor, hypokalemia, increased lactic acid, headache, and hyperglycemia. 
     Chromolyn sodium and nedocromil are used as long-term control medications for preventing primarily asthma symptoms arising from exercise or allergic symptoms arising from allergens. These compounds are believed to block early and late reactions to allergens by interfering with chloride channel function. They also stabilize mast cell membranes and inhibit activation and release of mediators from inosineophils and epithelial cells. A four to six week period of administration is generally required to achieve a maximum benefit. 
     Anticholinergics are generally used for the relief of acute bronchospasm. These compounds are believed to function by competitive inhibition of muscarinic cholinergic receptors. Anticholinergics include, but are not limited to, ipratropium bromide. These compounds reverse only cholinerigically-mediated bronchospasm and do not modify any reaction to antigen. Side effects include drying of the mouth and respiratory secretions, increased wheezing in some individuals, and blurred vision if sprayed in the eyes. 
     In some embodiments, provided immunomodulatory oligonucleotide compositions may also be useful for treating airway remodeling. Airway remodeling results from smooth muscle cell proliferation and/or submucosal thickening in the airways, and ultimately causes narrowing of the airways leading to restricted airflow. The immunomodulatory oligonucleotides of the invention may prevent further remodeling and possibly even reduce tissue build-up resulting from the remodeling process. 
     In some embodiments, provided immunomodulatory oligonucleotide compositions are useful for treating a subject having an inflammatory disorder. As used herein, the term “inflammatory disorder” refers to a condition associated with an antigen-nonspecific reaction of the innate immune system that involves accumulation and activation of leukocytes and plasma proteins at a site of infection, toxin exposure, or cell injury. Cytokines that are characteristic of inflammation include tumor necrosis factor (TNF-α), interleukin 1 (IL-1), IL-6, IL-12, interferon alpha (IFN-α), interferon beta (IFN-β), and chemokines. Thus, certain types of asthma, allergy, and autoimmune disorders may have characteristics of an inflammatory disorder. Inflammatory disorders also include, for example cardiovascular disease, chronic obstructive pulmonary disease (COPD), bronchiectasis, chronic cholecystitis, tuberculosis, Hashimoto&#39;s thyroiditis, sepsis, sarcoidosis, silicosis and other pneumoconiosis, and an implanted foreign body in a wound, but are not so limited. As used herein, the term “sepsis” refers to a well-recognized clinical syndrome associated with a host&#39;s systemic inflammatory response to microbial invasion. The term “sepsis” as used herein refers to a condition that is typically signaled by fever or hypothermia, tachycardia, and tachypnea, and in severe instances can progress to hypotension, organ dysfunction, and even death. 
     In some embodiments, provided immunomodulatory oligonucleotide compositions are useful for treating a subject having an infection. In some embodiments, provided immunomodulatory oligonucleotide compositions are useful for treating a subject who is susceptible to an infection, including those who may have exposure to a pathogen or pathogens. The immunomodulatory oligonucleotides utilized in accordance with the invention can in some aspects also be used to treat or prevent infections by viruses, bacteria, fungi, or parasites. A subject having an infection is a subject that has been exposed to an infectious pathogen and has acute or chronic detectable levels of the pathogen in the body. The immunomodulatory oligonucleotides can be used with or without an antigen to mount an antigen specific systemic or mucosal immune response that is capable of reducing the level of or eradicating the infectious pathogen. An infectious disease, as used herein, is a disease arising from the presence of a foreign microorganism in the body. It is particularly important to develop effective vaccine strategies and treatments to protect the body&#39;s mucosal surfaces, which are the primary site of pathogenic entry. 
     Viruses are small infectious agents which generally contain a nucleic acid core and a protein coat, but are not independently living organisms. Viruses can also take the form of infectious nucleic acids lacking a protein. A virus cannot survive in the absence of a living cell within which it can replicate. Viruses enter specific living cells either by endocytosis or direct injection of DNA (phage) and multiply, causing disease. The multiplied virus can then be released and infect additional cells. Some viruses are DNA-containing viruses and others are RNA-containing viruses. DNA viruses include Pox, Herpes, Adeno, Papova, Parvo, and Hepadna. RNA viruses include Picorna, Calici, Astro.Toga, Flavi, Corona, Paramyxo, Orthomyxo, Bunya, Arena, Rhabdo, FiIo, Borna, Reo, and Retro. In some aspects, the invention also intends to treat diseases in which prions are implicated in disease progression such as for example bovine spongiform encephalopathy (i.e., mad cow disease, BSE) or scrapie infection in animals, or Creutzfeldt-Jakob disease in humans. 
     Viruses include, but are not limited to, enteroviruses (including, but not limited to, viruses that the family picornaviridae, such as polio virus, Coxsackie virus, echo virus), rotaviruses, adenovirus, and hepatitis virus, such as hepatitis A, B, C D and E. Specific examples of viruses that have been found in humans include but are not limited to: Retroviridae (e.g., human immunodeficiency viruses, such as HIV-1 (also referred to as HTLV-III, LAV or HTLV-III/LAV, or HIV-III; and other isolates, such as HIV-LP; Picornaviridae (e.g., polio viruses, hepatitis A virus; enteroviruses, human Coxsackie viruses, rhinoviruses, echoviruses); Calciviridae (e.g., strains that cause gastroenteritis); Togaviridae (e.g., equine encephalitis viruses, rubella viruses); Flaviviridae (e.g., dengue viruses, encephalitis viruses, yellow fever viruses); Coronaviridae (e.g., coronavi ruses); Rhabdoviridae (e.g., vesicular stomatitis viruses, rabies viruses); Filoviridae (e.g., ebola viruses); Paramyxoviridae (e.g., parainfluenza viruses, mumps virus, measles virus, respiratory syncytial virus); Orthomyxoviridae (e.g., influenza viruses); Bunyaviridae (e.g., Hantaan viruses, bunya viruses, phleboviruses and Nairo viruses); Arenaviridae (hemorrhagic fever viruses); Reoviridae (e.g., reoviruses, orbiviurses and rotaviruses); Birnaviridae; Hepadnaviridae (Hepatitis B virus); Parvoviridae (parvoviruses); Papovavihdae (papillomaviruses, polyoma viruses); Adenoviridae (most adenoviruses); Herpesviridae (herpes simplex virus (HSV) 1 and 2, varicella zoster virus, cytomegalovirus (CMV)); Poxviridae (variola viruses, vaccinia viruses, pox viruses); Iridoviridae (e.g., African swine fever virus); and other viruses acute laryngotracheobronchitis virus, Alphavirus, Kaposi&#39;s sarcoma-associated herpesvirus, Newcastle disease virus, Nipah virus, Norwalk virus, Papillomavirus, parainfluenza virus, avian influenza, SARs virus, West Nile virus. Viruses that infect plants include, for example, viruses of the genera and/or families of Alfamoviruses: Bromoviridae, Alphacryptoviruses: Partitiviridae, Badnaviruses, Betacryptoviruses: Partitiviridae, Bigeminiviruses: Geminiviridae, Bromoviruses: Bromoviridae, Bymoviruses: Potyviridae, Capilloviruses, Carlaviruses, Carmoviruses: Tombusviridae, Caulimoviruses, Closteroviruses, Comoviruses: Comoviridae, Cucumoviruses: Bromoviridae, Cytorhabdoviruses: Rhabdoviridae, Dianthoviruses, Enamoviruses, Fabaviruses: Comoviridae, Fijiviruses: Reoviridae, Furoviruses, Hordeiviruses, Hybrigeminiviruses: Geminiviridae, Idaeoviruses, Ilarviruses: Bromoviridae, Ipomoviruses: Potyviridae, Luteoviruses, Machlomoviruses, Macluraviruses, Marafiviruses, Monogeminiviruses: Geminiviridae, Nanaviruses, Necroviruses, Nepoviruses: Comoviridae, Nucleorhabdoviruses: Rhabdoviridae, Oryzaviruses: Reoviridae, Ourmiaviruses, Phytoreoviruses: Reoviridae, Potexviruses, Potyviruses: Potyviridae, Rymoviruses: Potyviridae, Satellite RNAs, Satelliviruses, Sequiviruses: Sequiviridae, Sobemoviruses, Tenuiviruses, Tobamoviruses, Tobraviruses, Tombusviruses: Tombusviridae, Tospoviruses: Bunyaviridae, Trichoviruses, Tymoviruses, Umbraviruses, Unassigned potyviruses: Potyviridae, Unassigned rhabdoviruses: Rhabdoviridae, Varicosaviruses, Waikaviruses: Sequiviridae, Ungrouped viruses, Bromoviridae Potyviridae and Tymoviridae; particular relevant plant viruses include, for example, Tobacco mosaic virus (TMV), Tomato spotted wilt virus, Tomato yellow leaf curl virus, Cucumber mosaic virus, Potato virus Y, Cauliflower mosaic virus, African cassava mosaic virus, Plum pox virus, Brome mosaic virus, Potato virus X, Citrus tristeza virus, Barley yellow dwarf virus, Potato leafroll virus and Tomato bushy stunt virus. 
     Viral hepatitis is an inflammation of the liver which may produce swelling, tenderness, and sometimes permanent damage to the liver. If the inflammation of the liver continues at least six months or longer, it is referred to as chronic hepatitis. There are at least five different viruses known to cause viral hepatitis, include hepatitis A 1 B, C D and E. Hepatitis A is generally communicated through food or drinking water contaminated with human feces. Hepatitis B generally is spread through bodily fluids such as blood. For instance, it may be spread from mother to child at birth, through sexual contact, contaminated blood transfusions and needles. Hepatitis C is quite common and like Hepatitis B is often spread through blood transfusions and contaminated needles. Hepatitis D is found most often in IV drug users who are carriers of the hepatitis B virus with which it co-associates. Hepatitis E is similar to viral hepatitis A and is generally associated with poor sanitation. 
     Both gram negative and gram positive bacteria serve as antigens in vertebrate animals. Such gram positive bacteria include, but are not limited to,  Pasteυrella  species, Staphylococci species, and  Streptococcus  species. Gram negative bacteria include, but are not limited to,  Escherichia coli, Pseudomonas  species, and  Salmonella  species. Specific examples of infectious bacteria include but are not limited to,  Helicobacter pyloris, Borelia burgdorferi, Legionella pneumophilia, Mycobacteria  sps (e.g.  M. tuberculosis, M. avium, M. intracellular, M. kansaii, M. gordonae ),  Staphylococcus aureus, Neisseria gonorrhoeae, Neisseria meningitidis, Listeria monocytogenes, Streptococcus pyogenes  (Group A  Streptococcus ),  Streptococcus agalactiae  (Group B  Streptococcus ),  Streptococcus  ( viridans  group),  Streptococcus faecalis, Streptococcus bovis, Streptococcus  ( anaerobic  sps.),  Streptococcus pneumoniae , pathogenic  Campylobacter  sp.,  Enterococcus  sp.,  Haemophilus influenzae, Bacillus antracis, Corynebacterium diphtheriae, Corynebacterium  sp.,  Erysipelothrix rhusiopathiae, Clostridium penringers, Clostridium tetani, Enterobacter aerogenes, Klebsiella pneumoniae, Pasteurella multocida, Bacteroides  sp.,  Fusobacterium nucleatum,    
       Streptobacillus moniliformis, Treponema pallidium, Treponema pertenue, Leptospira, Rickettsia , and  Actinomyces israelii.    
     Examples of fungi include  Cryptococcus neoformans, Histoplasma capsulatum, Coccidioides immitis, Blastomyces dermatitidis, Chlamydia trachomatis, Candida albicans.    
     Other infectious organisms (i.e., protists) include  Plasmodium  spp. such as  Plasmodium falciparum, Plasmodium malariae, Plasmodium ovale , and  Plasmodium vivax  and  Toxoplasma gondii . Blood-borne and/or tissues parasites include  Plasmodium  spp.,  Babesia microti, Babesia divergens, Leishmania tropica, Leishmania  spp.,  Leishmania braziliensis, Leishmania donovani, Trypanosoma gambiense  and  Trypanosoma rhodesiense  (African sleeping sickness),  Trypanosoma cruzi  (Chagas&#39; disease), and  Toxoplasma gondii.    
     Other medically relevant microorganisms have been described extensively in the literature, e.g., see C. G. A Thomas, Medical Microbiology, Bailliere Tindall, Great Britain 1983, the entire contents of which is hereby incorporated by reference. Agriculturally relevant microorganisms (e.g., those that infect crops and/or livestock) are also known, for example but not limited to those described in, e.g., George N. Agrios, Plant Pathology, Elsevier Academic Press, 5 th  Edition, 2005; John Lucas, Plant Pathology and Plant Pathogens, Wiley-Blackwell; 3 rd  edition, 1998; Dwight C. Hirsh, N. James MacLachlan, Richard L. Walker, Veterinary Microbiology, Wiley-Blackwell; 2 edition, 2004; and P. J. Quinn, et al, Veterinary Microbiology and Microbial Disease, Wiley-Blackwell; 2 edition, 2011. 
     Provided oligonucleotide compositions of the invention may be administered to a subject (including, for example, a human subject or, in some embodiments an animal [e.g., livestock or pet animal] or plant [e.g., crop] subject) with an anti-microbial agent. An anti-microbial agent, as used herein, refers to a naturally-occurring or synthetic compound which is capable of killing or inhibiting infectious microorganisms. The type of anti-microbial agent useful according to the invention will depend upon the type of microorganism with which the subject is infected or at risk of becoming infected. Anti-microbial agents include but are not limited to anti-bacterial agents, anti-viral agents, anti-fungal agents and anti-parasitic agents. Phrases such as “anti-infective agent”, “anti-bacterial agent”, “anti-viral agent”, “anti-fungal agent”, “antiparasitic agent” and “parasiticide” have well-established meanings to those of ordinary skill in the art and are defined in standard medical texts. Briefly, anti-bacterial agents kill or inhibit bacteria, and include antibiotics as well as other synthetic or natural compounds having similar functions. Antibiotics are low molecular weight molecules which are produced as secondary metabolites by cells, such as microorganisms. In general, antibiotics interfere with one or more bacterial functions or structures which are specific for the microorganism and which are not present in host cells. Anti-viral agents can be isolated from natural sources or synthesized and are useful for killing or inhibiting viruses. Anti-fungal agents are used to treat superficial fungal infections as well as opportunistic and primary systemic fungal infections. Anti-parasite agents kill or inhibit parasites. 
     Examples of anti-parasitic agents, also referred to as parasiticides useful for human administration include but are not limited to albendazole, amphotericin B, benznidazole, bithionol, chloroquine HCI, chloroquine phosphate, clindamycin, dehydroemetine, diethylcarbamazine, diloxanide furoate, eflornithine, furazolidaone, glucocorticoids, halofantrine, iodoquinol, ivermectin, mebendazole, mefloquine, meglumine antimoniate, melarsoprol, metrifonate, metronidazole, niclosamide, nifurtimox, oxamniquine, paromomycin, pentamidine isethionate, piperazine, praziquantel, primaquine phosphate, proguanil, pyrantel pamoate, pyrimethanmine-sulfonamides, pyrimethanmine-sulfadoxine, quinacrine HCI, quinine sulfate, quinidine gluconate, spiramycin, stibogluconate sodium (sodium antimony gluconate), suramin, tetracycline, doxycycline, thiabendazole, tinidazole, trimethroprim-sulfamethoxazole, and tryparsamide some of which are used alone or in combination with others. 
     Antibacterial agents kill or inhibit the growth or function of bacteria. A large class of antibacterial agents is antibiotics. Antibiotics, which are effective for killing or inhibiting a wide range of bacteria, are referred to as broad spectrum antibiotics. Other types of antibiotics are predominantly effective against the bacteria of the class gram-positive or gram-negative. These types of antibiotics are referred to as narrow spectrum antibiotics. Other antibiotics which are effective against a single organism or disease and not against other types of bacteria, are referred to as limited spectrum antibiotics. Antibacterial agents are sometimes classified based on their primary mode of action. In general, antibacterial agents are cell wall synthesis inhibitors, cell membrane inhibitors, protein synthesis inhibitors, nucleic acid synthesis or functional inhibitors, and competitive inhibitors. 
     Antiviral agents are compounds which prevent infection of cells by viruses or replication of the virus within the cell. There are many fewer antiviral drugs than antibacterial drugs because the process of viral replication is so closely related to DNA replication within the host cell, that non-specific antiviral agents would often be toxic to the host. There are several stages within the process of viral infection which can be blocked or inhibited by antiviral agents. These stages include, attachment of the virus to the host cell (immunoglobulin or binding peptides), uncoating of the virus (e.g. amantadine), synthesis or translation of viral mRNA (e.g. interferon), replication of viral RNA or DNA (e.g. nucleotide analogues), maturation of new virus proteins (e.g. protease inhibitors), and budding and release of the virus. 
     Nucleotide analogues are synthetic compounds which are similar to nucleotides, but which have an incomplete or abnormal deoxyribose or ribose group. Once the nucleotide analogues are in the cell, they are phosphorylated, producing the triphosphate formed which competes with normal nucleotides for incorporation into the viral DNA or RNA. Once the triphosphate form of the nucleotide analogue is incorporated into the growing nucleic acid chain, it causes irreversible association with the viral polymerase and thus chain termination. Nucleotide analogues include, but are not limited to, acyclovir (used for the treatment of herpes simplex virus and varicella-zoster virus), gancyclovir (useful for the treatment of cytomegalovirus), idoxuridine, ribavirin (useful for the treatment of respiratory syncitial virus), dideoxyinosine, dideoxycytidine, zidovudine (azidothymidine), imiquimod, and resimiquimod. 
     The interferons are cytokines which are secreted by virus-infected cells as well as immune cells. The interferons function by binding to specific receptors on cells adjacent to the infected cells, causing the change in the cell which protects it from infection by the virus, α and β-interferon also induce the expression of Class I and Class II MHC molecules on the surface of infected cells, resulting in increased antigen presentation for host immune cell recognition, α and β-interferons are available as recombinant forms and have been used for the treatment of chronic hepatitis B and C infection. At the dosages which are effective for anti-viral therapy, interferons have severe side effects such as fever, malaise and weight loss. 
     Anti-viral agents useful in the invention include but are not limited to immunoglobulins, amantadine, interferons, nucleotide analogues, and protease inhibitors. Specific examples of anti-virals include but are not limited to Acemannan; Acyclovir; Acyclovir Sodium; Adefovir; Alovudine; Alvircept Sudotox; Amantadine Hydrochloride; Aranotin; Arildone; Atevirdine Mesylate; Avridine; Cidofovir; Cipamfylline; Cytarabine Hydrochloride; Delavirdine Mesylate; Desciclovir; Didanosine; Disoxaril; Edoxudine; Enviradene; Enviroxime; Famciclovir; Famotine Hydrochloride; Fiacitabine; Fialuridine; Fosarilate; Foscarnet Sodium; Fosfonet Sodium; Ganciclovir; Ganciclovir Sodium; Idoxuridine; Kethoxal; Lamivudine; Lobucavir; Memotine Hydrochloride; Methisazone; Nevirapine; Penciclovir; Pirodavir; Ribavirin; Rimantadine Hydrochloride; Saquinavir Mesylate; Somantadine Hydrochloride; Sorivudine; Statolon; Stavudine; Tilorone Hydrochloride; Trifluridine; Valacyclovir Hydrochloride; Vidarabine; Vidarabine Phosphate; Vidarabine Sodium Phosphate; Viroxime; Zalcitabine; Zidovudine; and Zinviroxime. 
     Anti-fungal agents are useful for the treatment and prevention of infective fungi. Anti-fungal agents are sometimes classified by their mechanism of action. Some antifungal agents function as cell wall inhibitors by inhibiting glucose synthase. These 
     include, but are not limited to, basiungin/ECB. Other anti-fungal agents function by destabilizing membrane integrity. These include, but are not limited to, immidazoles, such as clotrimazole, sertaconzole, fluconazole, itraconazole, ketoconazole, miconazole, and voriconacole, as well as FK 463, amphotericin B, BAY 38-9502, MK 991, pradimicin, UK 292, butenafine, and terbinafine. Other anti-fungal agents function by breaking down chitin (e.g. chitinase) or immunosuppression (501 cream). 
     In some embodiments, provided immunomodulatory oligonucleotide compositions are useful for treating a subject having a cell proliferative disease, such as cancer. A subject having a cancer is a subject that has detectable cancerous cells. The cancer may be a malignant or non-malignant cancer. Cancers or tumors include but are not limited to biliary tract cancer; brain cancer; breast cancer; cervical cancer; choriocarcinoma; colon cancer; endometrial cancer; esophageal cancer; gastric cancer; intraepithelial neoplasms; lymphomas; liver cancer; lung cancer (e.g. small cell and non-small cell); melanoma; neuroblastomas; oral cancer; ovarian cancer; pancreas cancer; prostate cancer; rectal cancer; sarcomas; skin cancer; testicular cancer; thyroid cancer; and renal cancer, as well as other carcinomas and sarcomas. In one embodiment the cancer is hairy cell leukemia, chronic myelogenous leukemia, cutaneous T-cell leukemia, multiple myeloma, follicular lymphoma, malignant melanoma, squamous cell carcinoma, renal cell carcinoma, prostate carcinoma, bladder cell carcinoma, or colon carcinoma. 
     Provided immunomodulatory oligonucleotide compositions may be administered alone or in conjunction with an anti-cancer therapy. Anti-cancer therapies include but are not limited to radiation therapy, chemotherapy, immunotherapy, a cancer vaccine, hormone therapy, a biological response modifier, and surgical procedures. A cancer medicament refers to an agent which is administered to a subject for the purpose of treating a cancer. As used herein, “treating cancer” includes preventing the development of a cancer, reducing the symptoms of cancer, and/or inhibiting the growth of an established cancer. In other aspects, the cancer medicament is administered to a subject at risk of developing a cancer for the purpose of reducing the risk of developing the cancer. Various types of medicaments for the treatment of cancer are described herein. For the purpose of this specification, cancer medicaments are classified as chemotherapeutic agents, immunotherapeutic agents, cancer vaccines, hormone therapy, and biological response modifiers. 
     Additionally, the methods of the invention are intended to embrace the use of more than one cancer medicament along with provided immunomodulatory oligonucleotide compositions. As an example, where appropriate, provided immunomodulatory oligonucleotide compositions may be administered with both a chemotherapeutic agent and an immunotherapeutic agent. Alternatively, the cancer medicament may embrace an immunotherapeutic agent and a cancer vaccine, or a chemotherapeutic agent and a cancer vaccine, or a chemotherapeutic agent, an immunotherapeutic agent and a cancer vaccine all administered to one subject for the purpose of treating a subject having a cancer or at risk of developing a cancer. 
     Chemotherapeutic agent may be selected from the group consisting of methotrexate, vincristine, adriamycin, cisplatin, non-sugar containing chloroethylnitrosoureas, 5-fluorouracil, mitomycin C, bleomycin, doxorubicin, dacarbazine, taxol, fragyline, Meglamine GLA, valrubicin, carmustaine and poliferposan, MMI270, BAY 12-9566, RAS famesyl transferase inhibitor, famesyl transferase inhibitor, MMP, MTA/LY231514, LY264618/Lometexol, Glamolec, CI-994, TNP-470, Hycamtin/Topotecan, PKC412, Valspodar/PSC833, Novantrone/Mitroxantrone, Metaret/Suramin, Batimastat, E7070, BCH-4556, CS-682, 9-AC, AG3340, AG3433, lncel/VX-710, VX-853, ZD0101, ISI641, ODN 698, TA 2516/Marmistat, BB2516/Marmistat, CDP 845, D2163, PD183805, DX8951f, Lemonal DP 2202, FK 317, Picibanil/OK-432, AD 32/Valrubicin, Metastron/strontium derivative, Temodal/Temozolomide, Evacet/liposomal doxorubicin, Yewtaxan/Paclitaxel, Taxol/Paclitaxel, Xeload/Capecitabine, Furtulon/Doxifluridine, Cyclopax/oral paclitaxel, Oral Taxoid, SPU-077/Cisplatin, HMR 1275/Flavopiridol, CP-358 (774)/EGFR, CP-609 (754)/RAS oncogene inhibitor, BMS-182751/oral platinum, UFT (Tegafur/Uracil), Ergamisol/Levamisole, Eniluracil/776C85/5FU enhancer, Campto/Levamisole, Camptosar/lrinotecan, Tumodex/Ralitrexed, Leustatin/Cladribine, Paxex/Paclitaxel, Doxil/liposomal doxorubicin, Caelyx/liposomal doxorubicin, Fludara/Fludarabine, Pharmarubicin/Epirubicin, DepoCyt, ZD1839, LU 79553/Bis-Naphtalimide, LU 103793/Dolastain, Caetyx/liposomal doxorubicin, Gemzar/Gemcitabine, ZD 0473/Anormed, YM 116, Iodine seeds, CDK4 and CDK2 inhibitors, PARP inhibitors, D4809/Dexifosamide, Ifes/Mesnex/lfosamide, Vumon/Teniposide, Paraplatin/Carboplatin, Plantinol/cisplatin, Vepeside/Etoposide, ZD 9331, Taxotere/Docetaxel, prodrug of guanine arabinoside, Taxane Analog, nitrosoureas, alkylating agents such as melphelan and cyclophosphamide, Aminoglutethimide, Asparaginase, Busulfan, Carboplatin, Chlorambucil, Cytarabine HCI, Dactinomycin, Daunorubicin HCI, Estramustine phosphate sodium, Etoposide (VP16-213), Floxuridine, Fluorouracil (5-FU), Flutamide, Hydroxyurea (hydroxycarbamide), Ifosfamide, Interferon Alfa-2a, Alfa-2b, Leuprolide acetate (LHRH-releasing factor analogue), Lomustine (CCNU), Mechlorethamine HCI (nitrogen mustard), Mercaptopurine, Mesna, Mitotane (o.p′-DDD), Mitoxantrone HCI, Octreotide, Plicamycin, Procarbazine HCI, Streptozocin, 
     Tamoxifen citrate, Thioguanine, Thiotepa, Vinblastine sulfate, Amsacrine (m-AMSA), Azacitidine, Erthropoietin, Hexamethylmelamine (HMM), Interleukin 2, Mitoguazone (methyl-GAG; methyl glyoxal bis-guanylhydrazone; MGBG), Pentostatin (2′deoxycoformycin), Semustine (methyl-CCNU), Teniposide (VM-26) and Vindesine sulfate, but it is not so limited. 
     Immunotherapeutic agent may be selected from the group consisting of Ributaxin, Herceptin, Quadramet, Panorex, IDEC-Y2B8, BEC2, C225, Oncolym, SMART M195, ATRAGEN, Ovarex, Bexxar, LDP-03, ior tβ, MDX-210, MDX-11, MDX-22, OV103, 3622W94, anti-VEGF, Zenapax, MDX-220, MDX-447, MELIMMUNE-2, MELIMMUNE-1, CEACIDE, Pretarget, NovoMAb-G2, TNT, Gliomab-H, GNI-250, EMD-72000, LymphoCide, CMA 676, Monopharm-C, 4B5, ior egf.r3, ior c5, BABS, anti-FLK-2, MDX-260, ANA Ab, SMART 1 D10 Ab, SMART ABL 364 Ab and ImmuRAIT-CEA, but it is not so limited. 
     Cancer vaccine may be selected from the group consisting of EGF, Anti-idiotypic cancer vaccines, Gp75 antigen, GMK melanoma vaccine, MGV ganglioside conjugate vaccine, Her2/neu, Ovarex, M-Vax, O-Vax, L-Vax, STn-KHL theratope, BLP25 (MUC-1), liposomal idiotypic vaccine, Melacine, peptide antigen vaccines, toxin/antigen vaccines, MVA-based vaccine, PACIS, BCG vacine, TA-HPV, TA-CIN, DISC-virus and ImmuCyst/TheraCys, but it is not so limited. 
     As used herein, the terms “cancer antigen” and “tumor antigen” are used interchangeably to refer to antigens which are differentially expressed by cancer cells and can thereby be exploited in order to target cancer cells. Cancer antigens are antigens which can potentially stimulate apparently tumor-specific immune responses. Some of these antigens are encoded, although not necessarily expressed, by normal cells. These antigens can be characterized as those which are normally silent (i.e., not expressed) in normal cells, those that are expressed only at certain stages of differentiation and those that are temporally expressed such as embryonic and fetal antigens. Other cancer antigens are encoded by mutant cellular genes, such as oncogenes (e.g., activated ras oncogene), suppressor genes (e.g., mutant p53), fusion proteins resulting from internal deletions or chromosomal translocations. Still other cancer antigens can be encoded by viral genes such as those carried on RNA and DNA tumor viruses. 
     In some embodiments, provided immunomodulatory oligonucleotide compositions may also be useful for treating and preventing autoimmune disease. Autoimmune disease is a class of diseases in which a subject&#39;s own antibodies react with host tissue or in which immune effector T cells are autoreactive to endogenous self peptides and cause destruction of tissue. Thus an immune response is mounted against a subject&#39;s own antigens, referred to as self antigens. Autoimmune diseases include but are not limited to alopecia areata, acquired hemophilia, ankylosing spondylitis, antiphospholipid syndrome, autoimmune-associated infertility, autoimmune encephalomyelitis, autoimmune hepatitis, autoimmune hemolytic anemia, autoimmune diabetes mellitus, autoimmune thrombocytopenic purpura, Behget&#39;s syndrome, bullous pemphigoid, cardiomyopathy, chronic fatigue immune dysfunction syndrome (CFIDS), chronic inflammatory demyelinating polyneuropathy, Churg-Strauss syndrome, cicatricial pemphigoid, CREST syndrome, cold agglutinin disease, dermatomyositis, discoid lupus, essential mixed cryoglobulinemia, fibromyalgia, fibromyositis, Guillain-Barre syndrome, Hashimoto&#39;s thyroiditis, glomerulonephritis (e.g., crescentic glomerulonephritis, proliferative glomerulonephritis), Grave&#39;s disease, graft versus host disease, Goodpasture&#39;s syndrome, pemphigus (e.g., pemphigus vulgaris), idiopathic pulmonary fibrosis, idiopathic thrombocytopenic purpura, insulin resistance, idiopathic Addison&#39;s disease, IgA nephropathy, inflammatory bowel disease (including Crohn&#39;s disease and ulcerative colitis), juvenile arthritis, lichen planus, myasthenia gravis, multiple sclerosis, mixed connective tissue disease, polymyositis, pernicious anemia, polyarteritis nodosa, polychondritis, polyglandular syndromes, polymyalgia rheumatica, primary agammaglobulinemia, primary biliary cirrhosis, psoriasis, Raynaud&#39;s phenomena, Reite{circumflex over ( )}s syndrome, juvenile and adult rheumatoid arthritis, Sjorgen&#39;s syndrome, scleroderma with anti-collagen antibodies, sarcoidosis, stiff-man syndrome, systemic lupus erythematosus (SLE), Takayasu arthritis, transplanted organ rejection, temporal arteritis/giant cell arteritis, uveitis, ulcerative colitis, vasculitis, and vitiligo. 
     A “self-antigen” as used herein refers to an antigen of a normal host tissue. Normal host tissue does not include cancer cells. Thus an immune response mounted against a self-antigen, in the context of an autoimmune disease, is an undesirable immune response and contributes to destruction and damage of normal tissue, whereas an immune response mounted against a cancer antigen is a desirable immune response and contributes to the destruction of the tumor or cancer. Thus, in some aspects of the invention aimed at treating autoimmune disorders it is not recommended that the immunomodulatory oligonucleotides be administered with self antigens, particularly those that are the targets of the autoimmune disorder. 
     In other instances, immunomodulatory oligonucleotides used in according to the present invention may be delivered with low doses of self-antigens. A number of animal studies have demonstrated that mucosal administration of low doses of antigen can result in a state of immune hyporesponsiveness or “tolerance.” The active mechanism appears to be a cytokine-mediated immune deviation away from a Th1 towards a predominantly Th2 and Th3 (i.e., TGF-β dominated) response. The active suppression with low dose antigen delivery can also suppress an unrelated immune response (bystander suppression) which is of considerable interest in the therapy of autoimmune diseases, for example, rheumatoid arthritis and SLE. Bystander suppression involves the secretion of Th1-counter-regulatory, suppressor cytokines in the local environment where proinflammatory and Th1 cytokines are released in either an antigen-specific or antigen-nonspecific manner. ‘Tolerance” as used herein is used to refer to this phenomenon. Indeed, oral tolerance has been effective in the treatment of a number of autoimmune diseases in animals including: experimental autoimmune encephalomyelitis (EAE), experimental autoimmune myasthenia gravis, collagen-induced arthritis (CIA), and insulin-dependent diabetes mellitus. In these models, the prevention and suppression of autoimmune disease is associated with a shift in antigen-specific humoral and cellular responses from a Th1 to Th2/Th3 response 
     Non-limiting examples of useful immunomodulatory oligonucleotides that can be utilized in accordance with the present invention are provided in the accompanying Appendix (B). 
     RIPtides 
     In some embodiments, oligonucleotides utilized in accordance with the present invention are or act as RNA-interacting polynucleotides (“RIPtides”). RIPtides are a class of therapeutics intended to open up the space of “undruggable” targets by inactivating structural RNAs implicated in disease. RIPtides are typically small nucleotide stretches (around 8 nucleotides) that bind and disrupt the action of structured RNA. Due to their size, these molecules can readily pass through the cell membrane. See, for example, U.S. Pat. No. 6,080,585. 
     RIPtides may be useful for a number of clinical applications. In the context of cancer therapy, at least one target is telomerase, which is present in about 90% of cancer cells. Therefore, in some embodiments, stereo-defined oligonucleotides of the invention can be used to target telomerase for the treatment of various cancer. In addition, RIPtides may be useful for the treatment of infectious disease. Viruses, such as HIV and Hepatitis C, must be able to take over a host cell in order to replicate; therefore, structural RNA can play an important role in the maturation and replication of many viruses. Accordingly, in some embodiments, stereo-defined oligonucleotides of the invention can be used to target portions of DNA or RNA involved in viral maturation and/or replication. 
     Thus, the invention is useful for treating various viral infections. Viruses include, but are not limited to, enteroviruses (including, but not limited to, viruses that the family picornaviridae, such as polio virus, Coxsackie virus, echo virus), rotaviruses, adenovirus, and hepatitis virus, such as hepatitis A, B, C D and E. Specific examples of viruses that have been found in humans include but are not limited to: Retroviridae (e.g., human immunodeficiency viruses, such as HIV-1 (also referred to as HTLV-III, LAV or HTLV-III/LAV, or HIV-III; and other isolates, such as HIV-LP; Picornaviridae (e.g., polio viruses, hepatitis A virus; enteroviruses, human Coxsackie viruses, rhinoviruses, echoviruses); Calciviridae (e.g., strains that cause gastroenteritis); Togaviridae (e.g., equine encephalitis viruses, rubella viruses); Flaviviridae (e.g., dengue viruses, encephalitis viruses, yellow fever viruses); Coronaviridae (e.g., coronavi ruses); Rhabdoviridae (e.g., vesicular stomatitis viruses, rabies viruses); Filoviridae (e.g., ebola viruses); Paramyxoviridae (e.g., parainfluenza viruses, mumps virus, measles virus, respiratory syncytial virus); Orthomyxoviridae (e.g., influenza viruses); Bunyaviridae (e.g., Hantaan viruses, bunya viruses, phleboviruses and Nairo viruses); Arenaviridae (hemorrhagic fever viruses); Reoviridae (e.g., reoviruses, orbiviurses and rotaviruses); Birnaviridae; Hepadnaviridae (Hepatitis B virus); Parvoviridae (parvoviruses); Papovavihdae (papillomaviruses, polyoma viruses); Adenoviridae (most adenoviruses); Herpesviridae (herpes simplex virus (HSV) 1 and 2, varicella zoster virus, cytomegalovirus (CMV)); Poxviridae (variola viruses, vaccinia viruses, pox viruses); Iridoviridae (e.g., African swine fever virus); and other viruses acute laryngotracheobronchitis virus, Alphavirus, Kaposi&#39;s sarcoma-associated herpesvirus, Newcastle disease virus, Nipah virus, Norwalk virus, Papillomavirus, parainfluenza virus, avian influenza, SARs virus, West Nile virus. 
     Antisense Drugs 
     The present invention also encompasses a variety of antisense-based therapeutics. Typically, antisense drugs are small (12-21 nucleotides) DNA- or RNA-like compounds that are chemically modified to engineer favorable drug properties. Some of the antisense agents available in the art include phosphorothioate or deoxyoligonucleotides, and in this situation a sulfur group is exchanged for a nonbridging oxygen on the phosphate backbone. This increases the resistance to RNase degradation and improves the pharmacologic stability. Yet in other agents, so-called “the next generation of antisense molecules,” the oligonucleotides the 2′-O-methoxyethyl modification to the backbone, which can increase the affinity for these oligonucleotides for the target RNA. However, in some cases, many of these agents are associated with side effects, such as unwanted immune responses. At least in some situations, such undesirable properties of these agents are attributable at least in part to their stereorandom nature. The present invention provides stereodefined counterpart having preferred properties, such as improved lipophilicity and increased RNA binding. 
     A number of antisense-based therapeutics have been developed or in development. Non-limiting examples of antisense therapies are provided below. Stereo-defined (e.g., chirally pure) oligonucleotides as described herein can be prepared for achieving improved efficacy, increased affinity to target, reduced side effects, improved pharmacokinetics, enhanced stability and/or increased bioavailability, relative to the antisense drugs currently available in the art. In some embodiments, antisense therapeutics inclide Morpholino drugs. See, for example: Morcos, P A (2007). “Achieving targeted and quantifiable alteration of mRNA splicing with Morpholino oligos”. Biochem Biophys Res Commun 358 (2): 521-7. 
     Cytomegalovirus retinitis: Fomivirsen (marketed as Vitravene), was approved by the U.S. FDA in August 1998 as a treatment for cytomegalovirus retinitis. 
     Hemorrhagic fever viruses: In early 2006, scientists studying the Ebola hemorrhagic fever virus at USAMRIID announced a 75% recovery rate after infecting four rhesus monkeys and then treating them with an antisense Morpholino drug developed by AVI BioPharma, a U.S. biotechnology firm. [U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Md. News Release: Gene-Specific Ebola Therapies Protect Nonhuman Primates from Lethal Disease. Jan. 13, 2006] The usual mortality rate for monkeys infected with Ebola virus is 100%. In late 2008, AVI BioPharma successfully filed Investigational New Drug (IND) applications with the FDA for its two lead products for Marburg and Ebola viruses. These drugs, AVI-6002 (Lu, X; Yu, Q; Binder, GK; Chen, Z; Slepushkina, T; Rossi, J; Dropulic, B (2004). “Antisense-Mediated Inhibition of Human Immunodeficiency Virus (HIV) Replication by Use of an HIV Type 1-Based Vector Results in Severely Attenuated Mutants Incapable of Developing Resistance”. Journal of Virology 78 (13): 7079-88) and AVI-6003 are novel analogs based on AVI&#39;s PMO antisense chemistry in which anti-viral potency is enhanced by the addition of positively-charged components to the morpholino oligomer chain. Preclinical results of AVI-6002 and AVI-6003 demonstrated reproducible and high rates of survival in non-human primates challenged with a lethal infection of the Ebola and Marburg viruses, respectively (Medical News Today. AVI BioPharma Announces FDA Clears IND Applications For Clinical Trials Of RNA Therapeutic Agents For Treatment Of Ebola And Marburg Viruses. 30 Dec. 2008). 
     Cancer: Also in 2006, German physicians reported on a dose-escalation study for the compound AP 12009 (a phosphorothioate antisense oligodeoxynucleotide specific for the mRNA of human transforming growth factor TGF-beta2) in patients with high grade gliomas. At the time of the report, the median overall survival had not been obtained and the authors hinted at a potential cure (Results of G0004, a phase IIb actively controlled clinical trial with the TGF-b2 targeted compound AP 12009 for recurrent anaplastic astrocytoma—Hau et al. 24 (18 Supplement): 1566—ASCO Meeting Abstracts). 
     For example, trabedersen (AP 12009-P001) is developed as monotherapy for the treatment of patients with advanced pancreatic cancer, malignant melanoma, brain tumors, anaplastic astrocytoma, and colorectal carcinoma. AP 12009-P001 has been evaluated in an open-label, multicenter, Phase I/II dose escalation trial to evaluate safety and tolerability of i.v. administration of trabedersen in 61 patients with advanced solid tumors known to overproduce TGF-b2, who were either not or no longer amenable to established forms of therapies. 
     Other examples of antisense drugs that target proteins overexpressed in cancers include: Clusterin (OGX-011/TV01011), which regulates epithelial-mesenchymal transition induced by the growth factor TGF beta and the transcription factor TWIST1 in prostate cancer cells; ATL1101 (a second generation antisense inhibitor of the insulin-like growth factor-I receptor (IGF-IR)) in prostate cancer treatment; oblimersen (trade name: Genasense) for treating chronic lymphocytic leukemia; G4460 and LR3001 (inhibitors of c-myb) for the treatment of CML, melanoma, neuroblastoma, and cancers of the breast, pancreas and colon; MG98 (inhibitor of DNA methyltransferase I) for the treatment of renal cell carcinoma; ISIS 5132 (c-Raf antisense); LY900003 (Protein kinase C-alpha antisense); G3139 (Bcl-2 antisense), etc. 
     HIV/AIDS: Starting in 2004, researchers in the US have been conducting research on using antisense technology to combat HIV. It is known that antisense-mediated inhibition of human immunodeficiency virus (HIV) replication by use of an HIV type 1-based vector results in severely attenuated mutants incapable of developing resistance. In February 2010 researchers reported success in reducing HIV viral load using patient T-cells which had been harvested, modified with an RNA antisense strand to the HIV viral envelope protein, and re-infused into the patient during a planned lapse in retroviral drug therapy. 
     High cholesterol: In 2010 mipomersen (previously ISIS 301012, trade name Kynamro) successfully completed phase 3 trials for some types of high cholesterol. Mipomersen is a cholesterol-reducing drug candidate. It is an antisense therapeutic that targets the messenger RNA for apolipoprotein B. See: Merki E, Graham M J, Mullick A E, et al. (August 2008). “Antisense oligonucleotide directed to human apolipoprotein B-100 reduces lipoprotein(a) levels and oxidized phospholipids on human apolipoprotein B-100 particles in lipoprotein(a) transgenic mice”. Circulation 118 (7): 743-53; El Harchaoui K, Akdim F, Stroes E S, Trip M D, Kastelein J J (2008). “Current and future pharmacologic options for the management of patients unable to achieve low-density lipoprotein-cholesterol goals with statins”. Am J Cardiovasc Drugs 8 (4): 233-42; Athyros V G, Kakafika A I, Tziomalos K, Karagiannis A, Mikhailidis D P (July 2008). “Antisense technology for the prevention or the treatment of cardiovascular disease: the next blockbuster?”. Expert Opin Investig Drugs 17 (7): 969-72. It is administered as a weekly injection. The compound is a ‘second-generation’ antisense oligonucleotide; the nucleotides are linked with phosphorothioate linkages rather than the phosphodiester linkages of RNA and DNA, and the sugar parts are deoxyribose in the middle part of the molecule and 2′-O-methoxyethyl-modified ribose at the two ends. These modifications make the drug resistant to degradation by nucleases, allowing it to be administered weekly. The drug accumulates in the liver, which is convenient since apolipoprotein B predominantly acts there. The complete sequence is  G *- C *-C*- U *-C*-d A -dG-d T -dC-dT-dG-d mC -dT-dT-dmC-G*-C*-A*-C*-C* (SEQ ID NO: 113) [d=2′-deoxy, *=2′-O-(2-methoxyethyl)] with 3′-5′ phosphorothioate linkages. The Phase 3 trials were in patients with familial hypercholesterolemia (FH), both homozygous (ho) and heterozygous (he). FH is a genetic disorder that causes exceptionally high levels of low-density lipoprotein cholesterol. Both trials showed exceptional performance with the highest efficacy seen so far in those two patient populations, and with relatively low drop-out rates compared to other injectable drugs. 
     Duchenne muscular dystrophy: In Duchenne muscular dystrophy (DMD), the patient&#39;s muscle cells break down and are lost, leading to progressive muscle weakness and death. AVI-4658 is a targeted antisense therapy to restore expression of dystrophin, a key protein which patients with Duchenne muscular dystrophy lack. Results from an open-label, phase 2, dose-escalation study with 19 patients showed that when treatment was completed, a muscle biopsy was taken from each patient. The team discovered that the patients&#39; ability to produce functional mRNA through “exon skipping’ was repaired with the use of AVI-4658, eventually allowing them to manufacture functional dystrophin protein. 
     Myotonic dystrophy: Myotonic dystrophy is the most common muscular disease in adults, affecting mainly the skeletal muscles, heart and central nervous system. It occurs because of a mutation that causes numerous repeats of three letters of the genetic code (CTG) in a gene called DMPK. The messenger RNA that is produced from the mutated gene also contains the abnormal long repeats that cause the RNA to accumulate in the cell&#39;s nucleus. There it sequesters and blocks the function of a protein called Muscleblind-like 1 and activates another protein called CELF1. These proteins antagonize one another and the result is abnormal expression of proteins from many other genes in adult tissues, resulting in disease. To counteract this, Cooper and his colleagues created antisense oligonucleotides that are simply strands of genetic material that seek out portions of the abnormal RNA repeats and target RNase H to the toxic RNA causing its degradation. It was also reported that combining the antisense oligonucleotides with other antisense oligonucleotides that help released the sequestered Muscleblind-like1 can enhance the effect. 
     Diabetes: SGLT2 (ISIS 388626) is an antisense drug to achieve reduction in sodium dependent glucose co-transporter type 2 (SGLT2) levels that resulted in a significant reduction in blood glucose levels in diabetic patients. 
     Hepatitis: Persistent hepatitis C virus (HCV) infection is a leading cause of liver disease. Antisense oligonucleotides represent a promising class of antiviral agents. Such drugs under development include ISIS 14803, which is a 20-unit phosphorothioate oligodeoxynucleotide that inhibits HCV replication and protein expression. 
     Methods of Treatment 
     Provided oligonucleotides and compositions thereof, described herein, are useful as therapeutic agents against various disease states, including use as antiviral agents. In some embodiments, provided oligonucleotides can be used as agents for treatment of diseases through modulation of DNA and/or RNA activity. In some embodiments, provided oligonucleotides can be used for inhibiting specific gene expression. For example, a provided oligonucleotide can be complementary to a specific target messenger RNA (mRNA) sequence. It can be used to inhibit viral replication of myriad viruses. Exemplary virus families include orthomyxoviruses, pox viruses, herpes viruses, papilloma viruses, picornaviruses, flaviviruses, retroviruses, hepatitis viruses, paramyxoviruses, reoviruses, parvoviruses, filoviruses, coronaviruses, arenaviruses, rhabdoviruses and adenoviruses. Additional virus families are known and are also contemplated herein. Other examples include uses as antisense compounds against HIV RNA or other retroviral RNA or for hybridizing to HIV mRNA encoding the tat protein, or to the TAR region of HIV mRNA. In some embodiments, the nucleic acids mimic the secondary structure of the TAR region of HIV mRNA, and by doing so bind the tat protein. In some embodiments, a provided oligonucleotide is used to inhibit expression of a target protein by contacting a cell with a provided oligonucleotide, wherein the expression of other proteins in the cell are not inhibited or are minimally inhibited. In some embodiment, target protein inhibition occurs in vivo in a mammal. In some embodiments, a therapeutically effective amount of a provided oligonucleotide is administered for inhibiting the expression of a target protein. 
     Other examples of proteins where expression can be modulated include Jun N-terminal kinase (JNK) proteins, diacylglycerol acyltransferase I, apolipoprotein B, glucagon receptor, Aurora B, acyl CoA cholesterol acyltransferase-2, c-reactive protein, STAT (signal transducers and activators of transcription) family of proteins, and MDR P-glycoprotein. In some embodiments, a provided oligonucleotide can be used to inhibit protein phosphatase 1B (PTP1B) expression, RNA-dependent RNA viral polymerase. In some embodiments, a provided oligonucleotide can be used to induce events such as apoptosis in cancer cells or to make a cell more susceptible to apoptosis. In some embodiments, a provided oligonucleotide can be used to modulate activities of proteins. In some embodiments, a provided oligonucleotide can help modulate RNase H activity targeting multidrug resistance (MDR) RNA molecules. 
     In some embodiments, the present invention provides methods of treating a disease mediated by undesired gene expression in a subject (e.g., mammals, such as humans) in need of such treatment. By “diseases” is meant diseases, or disease symptoms. Methods include, e.g., administering to the subject an effective amount of a provided oligonucleotide. 
     Examples of diseases mediated by undesired gene expression include cancer (e.g., leukemia, tumors, and metastases), allergy, asthma, obesity, inflammation (e.g., inflammatory diseases such as inflammatory airways disease), hypercholesterolemia, hematological disorders, severe acute respiratory syndrome (SARS), obstructive airways disease, asthma, autoimmune diseases, retroviral diseases such as AIDS or HIV, other viral infections, intrauterine infections, metabolic diseases, infection (e.g., bacterial, viral, yeast, fungal), CNS diseases, brain tumors, degenerative neural diseases, cardiovascular diseases, and diseases associated with angiogenesis, neovascularization, and vasculogenesis. 
     In some embodiments, provided oligonucleotides are useful for treating cancer, including pancreatic cancer, and other diseases or disorders involving abnormal cell proliferation. 
     Located in the upper abdomen (in the retroperitoneum), the pancreas is a dual-function gland of the digestive and endocrine system. In certain instances, the pancreas functions as an endocrine gland (e.g., producing several important hormones). In certain instances, the pancreas functions as an exocrine gland (e.g., secreting fluids containing digestive enzymes that pass to the small intestine). 
     Pancreatic cancer is the fourth most common cause of cancer death in the US (after lung, colon and breast), comprising 6% of all cancer-related deaths. In 2008, an estimated 37,680 new cases of pancreatic cancer will have been diagnosed in the US, with 34,290 deaths. Incidence of the disease, rises linearly after age 50, with the only definitive risk factor being cigarette smoking (smokers are four times more likely to develop the disease than non-smokers). Invasive pancreatic cancer is almost always fatal. The collective median survival time of all patients is 4-6 months. Relative 1-year survival is 24%; the overall 5-year survival rate &lt;5%. 
     Pancreatic cancer is asymptomatic in its early stage and often remains undiagnosed for several months (less than one third of patients being diagnosed within 2 months of the onset symptoms). In certain instances, the delayed diagnosis results in (either partially or fully) metastasis of the cancerous cells to the liver or lymph nodes. 
     Currently, surgery (resectioning of the pancreas) is the primary and only curative therapy for pancreatic cancer. However, only 15-25% of tumors are resectable at the time of diagnosis and only 10-20% of patients undergoing surgery survive more than two years. Once tumor infiltration occurs and other tissues have been affected, surgery is no longer possible. 
     In certain instances, diabetes mellitus or pancreatitis predisposes an individual to develop a proliferative disorder of a plurality of pancreatic cells. In certain instances, individuals are at an increased risk of developing a proliferative disorder of a plurality of pancreatic cells due to a hereditary syndrome selected from the group consisting of hereditary nonpolyposis colorectal cancer (HNPCC) and familial adenomatous polyposis (FAP). In certain instances, individuals are at an increased risk of developing a proliferative disorder of a plurality of pancreatic cells due to a mutation in a gene selected from the group consisting of MSH2, MSH6, MLH1, and APC. 
     Ideally, effective treatment of pancreatic cancer should (i) control the primary tumor mass, both initially and subsequently, and (ii) treat the metastatic tumor cells. Chemoprevention (the administration of agents such as drugs, biologics, nutrients and the like) slows the progression of, reverses, or inhibits carcinogenesis, thereby lowering the risk of developing invasive or clinically significant disease. 
     Disclosed herein, in certain embodiments, are methods of treating pancreatic cancer. As used herein, “pancreatic cancer” includes forms of cancer of the pancreas. In some embodiments, a pancreatic cancer is metastatic pancreatic cancer. In some embodiments, a pancreatic cancer is a carcinoma, sarcoma, cancer, or combinations thereof. In some embodiments, a pancreatic cancer to be treated includes sporadic and hereditary pancreatic cancers. In some embodiments, a pancreatic cancer is duct cell carcinoma, acinar cell carcinoma, papillary mucinous carcinoma, signet ring carcinoma, adenosquamous carcinoma, undifferentiated carcinoma, mucinous carcinoma, giant cell carcinoma, small cell carcinoma, cystcancer, serous cystcancer, mucinous cystcancer, unclassified pancreatic cancer, pancreatoblastoma, or combinations thereof. 
     In some embodiments, an individual in need of treatment for pancreatic cancer presents with a localized tumor of the pancreas. In some embodiments, an individual in need of treatment for pancreatic cancer presents with a negative regional lymph node biopsy. In some embodiments, an individual in need of treatment for pancreatic cancer presents with a positive regional lymph node biopsy. In some embodiments, an individual in need of treatment for pancreatic cancer presents with a nodal negative pancreatic tumor (e.g., node-negative). In some embodiments, an individual in need of treatment for pancreatic cancer presents with a nodal positive tumor (e.g., node-positive). 
     In some embodiments, a pancreatic cancer in an individual in need of treatment for pancreatic cancer has metastasized to other locations in the body. In some embodiments, a pancreatic cancer has metastasized to a location selected from the group consisting of lymph node, stomach, bile duct, liver, bone, ovary, peritoneum and brain. 
     In some embodiments, cancer cells or precancerous cells are identified by histological typing or grading of a tissue sample (e.g., a biopsy sample). In some embodiments, cancer cells or precancerous cells are identified through the use of appropriate molecular markers. 
     In some embodiments, a pancreatic cancer in an individual in need of treatment for pancreatic cancer is staged according to the American Joint Committee on Cancer (AJCC) TNM classification system, where the tumor (T) has been assigned a stage of Tx, T1, T2, T3, T4; and where the regional lymph nodes (N) have been assigned a stage of NX, N0, N1; and where distant metastasis (M) has been assigned a stage of MX, M0, or M1. In some embodiments, a pancreatic cancer in an individual in need of treatment for pancreatic cancer is staged as Stage 0, I, IA, IB, II IIA, IIB, III, and IV pancreatic cancer. In some embodiments, a pancreatic cancer in an individual in need of treatment for pancreatic cancer is staged as Grade GX (e.g., grade cannot be assessed), Grade 1, Grade 2, Grade 3 or Grade 4. 
     More specific examples of cancers treated with provided oligonucleotides include breast cancer, lung cancer, melanoma, colorectal cancer, bladder cancer, ovarian cancer, prostate cancer, renal cancer, squamous cell cancer, glioblastoma, Kaposi&#39;s sarcoma, multiple myeloma, and leukemia. 
     Evaluation and Treatment of Cancer 
     The term “tumor cell antigen” is defined herein as an antigen that is present in higher quantities on a tumor cell or in body fluids than unrelated tumor cells, normal cells, or in normal body fluid. The antigen presence may be tested by any number of assays known to those skilled in the art and include without limitation negative and/or positive selection with antibodies, such as an ELISA assay, a radioimmunoassay, or by Western Blot. 
     “Apoptosis inducing agent” is defined herein to induce apoptosis/programmed cell death, and include, for example, anticancer agents and treatments wherein cells (e.g., tumor cells) are induced to undergo programmed cell death. Exemplary apoptosis inducing agents are described in more detail below. 
     The terms “apoptosis” or “programmed cell death,” refers to the physiological process by which unwanted or useless cells are eliminated during development and other normal biological processes. Apoptosis is a mode of cell death that occurs under normal physiological conditions and the cell is an active participant in its own demise (“cellular suicide”). It is most often found during normal cell turnover and tissue homeostasis, embryogenesis, induction and maintenance of immune tolerance, development of the nervous system and endocrine-dependent tissue atrophy. Cells undergoing apoptosis show characteristic morphological and biochemical features. These features include chromatin aggregation, nuclear and cytoplasmic condensation, partition of cytoplasm and nucleus into membrane bound vesicles (apoptotic bodies), which contain ribosomes, morphologically intact mitochondria and nuclear material. In vivo, these apoptotic bodies are rapidly recognized and phagocytized by macrophages, dendritic cells or adjacent epithelial cells. Due to this efficient mechanism for the removal of apoptotic cells in vivo no inflammatory response is elicited. In vitro, the apoptotic bodies as well as the remaining cell fragments ultimately swell and finally lyse. This terminal phase of in vitro cell death has been termed “secondary necrosis.” Apoptosis can be measured by methods known to those skilled in the art like DNA fragmentation, exposure of Annexin V, activation of caspases, release of cytochrome c, etc. A cell that has been induced to die is termed herein as an “apoptotic cell.” Apoptosis can also be tested using a standard Annexin V Apoptosis Assay: NIH:OVCAR-3 cells are grown in 6-well plates (NUNC) and irradiated or treated with an antagonist (or in combination with another anti-cancer drug) for 4-48 hours, washed and stained with Annexin V-FITC (BD-Pharmingen) for 1 hour. Cells are analyzed by flow cytometry (Becton-Dickinson, CellQuest), counterstained with Propidium Iodide and analyzed again in the flow cytometer. 
     Patients can be assessed with respect to symptoms at one or more multiple time points including prior to, during, and after treatment regimens. Treatment can result in improving the subject&#39;s condition and can be assessed by determining if one or more of the following factors has occurred: decreased tumor size, decreased cell proliferation, decreased numbers of cells, decreased neovascularization, increased apoptosis, or decreased survival of at least a portion of the tumor cells. One or more of these occurrences may, in some cases, result in partial or total elimination of the cancer and prolongation of survival of the patient. Alternatively, for terminal stage cancers, treatment may result in stasis of disease, better quality of life and/or prolongation of survival. 
     Methods of Assaying Cell Migration 
     Assays for cell migration have been described in the literature, e.g., by Brooks, et al., J. Clin. Invest 1997, 99:1390-1398 and methods for measuring cell migration are known to those of skill in the art. In one method for measuring cell migration described herein, membranes from transwell migration chambers are coated with substrate, the transwells washed, and non-specific binding sites blocked with BSA. Tumor cells from sub-confluent cultures are harvested, washed, and resuspended in migration buffer in the presence or absence of assay antibodies. After the tumor cells are allowed to migrate to the underside of the coated transwell membranes, the cells remaining on the top-side of the membrane are removed and cells that migrate to the under-side are stained with crystal violet. Cell migration is then quantified by direct cell counts per microscopic field. 
     Methods of Assaying Tumor Growth 
     Tumor growth can be assayed by methods known to those of skill in the art, e.g., the SCID mouse model, the nude mouse model, and BALB/c mice with syngeneic tumors. SCID mouse models for tumor growth are carried out as follows: subconfluent human M21 melanoma cells (or any desired tumor cell type) are harvested, washed, and resuspended in sterile PBS (20×106 per mL). SCID mice are injected subcutaneously with 100 L of M21 human melanoma cell (2×106) suspension. Three days after tumor cell injection, mice are either untreated or treated intraperitoneally with an antagonist in the desired dose ranges. The mice are treated daily for 24 days. Tumor size is measured with calipers and the volume estimated using the formula V=(L×W2)/2, where V is equal to the volume, L is equal to the length, and W is equal to the width. 
     Alternatively, nude mouse models, SCID mouse models and/or BALB/c syngeneic mouse models can also be utilized to assess tumor growth and inhibition thereof by the humanized anti-endoglin antibodies or antigen-binding fragments described herein. 
     Methods of Assaying Cell Proliferation 
     Cell proliferation can be assayed by methods known to those of skill in the art. As described herein, subconfluent human endothelial cells (HUVECs) can be resuspended in proliferation buffer containing low (5.0%) serum in the presence or absence of CM (25 μL) from ECV or ECVL cells, and endothelial cells allowed to proliferate for 24 hours. Proliferation can be quantified by measuring mitochondrial dehydrogenase activity using a commercially available WST-1 assay kit (Chemicon). Also, as described herein, proliferation can be quantified by measuring 3H incorporation using standard methods. (She et al., Int. J. Cancer, 108: 251-257 (2004)). 
     Other methods of assessing cell proliferation are known in the art and are contemplated herein. Further non-limiting examples are described in more detail in the examples. 
     One would understand that classification and staging systems described herein represent one means to assess treatment of cancers described herein; additionally, other staging schemes are known in the art and may be used in connection with the methods described herein. By way of example only, the TNM classification of malignant tumors may be used as a cancer staging system to describe the extent of cancer in a patient&#39;s body. T describes the size of the tumor and whether it has invaded nearby tissue, N describes regional lymph nodes that are involved, and M describes distant metastasis. TNM is maintained by the International Union Against Cancer (UICC) and is used by the American Joint Committee on Cancer (AJCC) and the International Federation of Gynecology and Obstetrics (FIGO). One would understand that not all tumors have TNM classifications such as, for example, brain tumors. Generally, T (a,is,(0), 1-4) is measured as the size or direct extent of the primary tumor. N (0-3) refers to the degree of spread to regional lymph nodes: N0 means that tumor cells are absent from regional lymph nodes, N1 means that tumor cells spread to the closest or small numbers of regional lymph nodes, N2 means that tumor cells spread to an extent between N1 and N3; N3 means that tumor cells spread to most distant or numerous regional lymph nodes. M (0/1) refers to the presence of metastasis: M0 means that no distant metastasis are present; M1 means that metastasis has occurred to distant organs (beyond regional lymph nodes). Other parameters may also be assessed. G (1-4) refers to the grade of cancer cells (i.e., they are low grade if they appear similar to normal cells, and high grade if they appear poorly differentiated). R (0/1/2) refers to the completeness of an operation (i.e., resection-boundaries free of cancer cells or not). L (0/1) refers to invasion into lymphatic vessels. V (0/1) refers to invasion into vein. C (1-4) refers to a modifier of the certainty (quality) of V. 
     Provided herein are methods for degrading, inhibiting the growth of or killing cancer cells comprising contacting the cells with an amount of a compound described herein effective to degrade, inhibit the growth of or kill cancer cells. 
     Provided herein are methods of inhibiting tumor size increase, reducing the size of a tumor, reducing tumor proliferation or preventing tumor proliferation in an individual comprising administering to said individual an effective amount of a compound described herein to inhibit tumor size increase, reduce the size of a tumor, reduce tumor proliferation or prevent tumor proliferation. Treatment of tumors in some cases includes stasis of symptoms, that is, by treating the patient, the cancer does not worsen and survival of the patient is prolonged. 
     Patients may be assessed with respect to symptoms at one or more multiple time points including prior to, during, and after treatment regimens. Treatment can result in improving the subject&#39;s condition and can be assessed by determining if one or more of the following events has occurred: decreased tumor size, decreased tumor cell proliferation, decreased numbers of cells, decreased neovascularization and/or increased apoptosis. One or more of these occurrences may, in some cases, result in partial or total elimination of the cancer and prolongation of survival of the patient. Alternatively, for terminal stage cancers, treatment may result in stasis of disease, better quality of life and/or prolongation of survival. 
     Other methods of assessing treatment are known in the art and contemplated herein. In an exemplary embodiment, the pro-oligonucleotide compounds of the invention are administered to a subject such as a mammal (e.g., a human), suffering from a medical disorder, e.g., a cancer, or non-malignant conditions characterized by the presence of a class of unwanted cells. 
     Primary outcome measures may be assessed for patients treated using the methods described herein and include, for example, progression-free survival. In one embodiment, an increase in progression free survival is observed in an amount of by about 2-fold, 5-fold, 10-fold, 20 fold, 50 fold or more compared to lack of treatment. In another embodiment, an increase in progression free survival is increased survival by about 3 months, about 6 months, about 9 months, about 12 months, about 18 months, about 2 years, about 3 years, about 4 years, about 5 years or more compared to lack of treatment. 
     Secondary outcome measures may also be assessed and include duration of response, time to tumor progression, overall survival, serious and non-serious adverse events. For example, a treatment may prevent progression of the disease (i.e., stasis) or may result in an improvement. Alternately, or in addition, other goals can be measured with respect to one or more of the following: decreased tumor burden, decreased neovascularization, reduced side effects, decreased adverse reactions, and/or increased patient compliance. 
     Other specific examples of diseases or disorders for which treatment by the compounds or compositions of the invention are useful for treatment or prevention include, but are not limited to transplant rejection (e.g., kidney, liver, heart, lung, islet cells, pancreas, bone marrow, cornea, small bowel, skin allografts or xenografts and other transplants), graft vs. host disease, osteoarthritis, rheumatoid arthritis, multiple sclerosis, diabetes, diabetic retinopathy, inflammatory bowel disease (e.g., Crohn&#39;s disease, ulcerative colitis, and other bowel diseases), renal disease, cachexia, septic shock, lupus, myasthenia gravis, psoriasis, dermatitis, eczema, seborrhea, Alzheimer&#39;s disease, Parkinson&#39;s disease, stem cell protection during chemotherapy, ex vivo selection or ex vivo purging for autologous or allogeneic bone marrow transplantation, ocular disease, retinopathies (e.g., macular degeneration, diabetic retinopathy, and other retinopathies), corneal disease, glaucoma, infections (e.g., bacterial, viral, or fungal), heart disease, including, but not limited to, restenosis. 
     Activation of RNAse L 
     The 2′-5′ oligoadenylate (2-5A)/RNase L pathway is one of the enzymatic pathways induced by interferon. Rnase L is activated after binding to 5′-phosphorylated fragments of 2′-5′ adenylic acid. These fragments of 2′-5′ adenylic acid (2-5A) are produced under the control of 2′-5′ oligo(A) synthetase. This pathway is part of the innate immune system and has an important role in preventing viral infection. 2-5A-Induced cleavage of single-stranded RNA results in apoptosis. Biostable phosphorothioate analogs of 2-5A have been shown to be potent activators of Rnase L (Xianh et al., Cancer Research (2003), 63:6795-6801). In this study, the 2-5A analogs induced Rnase L activity and caused apoptosis in cultures of late-stage, metastatic human prostate cancer cell lines DU145, PC3 and LNCaP. 
     Sustained activation of RNase L triggers a mitochondrial pathway of apoptosis that eliminates virus-infected cells as well as cancerous/tumor cells. RNase L can inhibit fibrosarcoma growth, prostate cancer growth, colorectal cancer growth and pancreatic cancer growth. Given the common role of RNase L in different cancers, it is contemplated that the invention described herein can be use for the treatment of any type of cancer. Silverman, R H, Cytokine Growth Factor Rev, 18(5-6): 381-388 (2007); Bisbal, C. and Silverman, R H,  Biochimie.  89(6-7): 789-798 (2007). By way of example, downregulation of RNase L refers to any reduction in expression levels of the gene or genes encoding RNase L, silencing of the gene or genes encoding RNase L, reduction in the levels of expression/translation of the proteins comprising RNase L, reduction in the amount of RNase L present within a cell, and/or any reduction in activity of RNase L as compared to a predetermined level of RNase L in an exemplary healthy population. Alternatively any reduction in RNase L levels as described herein can be indicative of downregulation of RNase L. 
     In some embodiments, provided oligonucleotides are useful for the treatment of diseases having downregulated RNase L. In some embodiment, a disease associated with downregulated RNase L is cancer. In some embodiments, the cancer is pancreatic cancer, prostate cancer, or colorectal cancer. Alternatively, provided oligonucleotides described herein are useful for the treatment of disease having upregulated RNase L. In some embodiments, the disease having upregulated RNase L is chronic fatigue syndrome. Additional diseases having upregulated RNase L are known in the art and contemplated herein. 
     When used as therapeutics, a provided oligonucleotide is administered as a pharmaceutical composition. In some embodiments, the pharmaceutical composition comprises a therapeutically effective amount of a provided oligonucleotide comprising, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable inactive ingredient selected from pharmaceutically acceptable diluents, pharmaceutically acceptable excipients, and pharmaceutically acceptable carriers. In another embodiment, the pharmaceutical composition is formulated for intravenous injection, oral administration, buccal administration, inhalation, nasal administration, topical administration, ophthalmic administration or otic administration. In further embodiments, the pharmaceutical composition is a tablet, a pill, a capsule, a liquid, an inhalant, a nasal spray solution, a suppository, a suspension, a gel, a colloid, a dispersion, a suspension, a solution, an emulsion, an ointment, a lotion, an eye drop or an ear drop. 
     Pharmaceutical Compositions 
     When used as therapeutics, a provided oligonucleotide or oligonucleotide composition described herein is administered as a pharmaceutical composition. In some embodiments, the pharmaceutical composition comprises a therapeutically effective amount of a provided oligonucleotides, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable inactive ingredient selected from pharmaceutically acceptable diluents, pharmaceutically acceptable excipients, and pharmaceutically acceptable carriers. In some embodiments, the pharmaceutical composition is formulated for intravenous injection, oral administration, buccal administration, inhalation, nasal administration, topical administration, ophthalmic administration or otic administration. In some embodiments, the pharmaceutical composition is a tablet, a pill, a capsule, a liquid, an inhalant, a nasal spray solution, a suppository, a suspension, a gel, a colloid, a dispersion, a suspension, a solution, an emulsion, an ointment, a lotion, an eye drop or an ear drop. 
     In some embodiments, the present invention provides a pharmaceutical composition comprising chirally controlled oligonucleotide, or composition thereof, in admixture with a pharmaceutically acceptable excipient. One of skill in the art will recognize that the pharmaceutical compositions include the pharmaceutically acceptable salts of the chirally controlled oligonucleotide, or composition thereof, described above. 
     Compounds for Enhancing and Targeting Delivery 
     A provided oligonucleotide as described herein can be delivered using a variety of delivery strategies, including conjugates of nucleic acids with various ligands, as well as use of nanocarrier approaches. Any nucleic acid delivery strategies are contemplated for use with provided oligonucleotides described herein. The choice between exemplary delivery strategies, including but not limited to, chemical conjugates, cationic lipid/liposomal transfer vessicles and supramolecular nanocarriers depends on the therapeutic context, and methods for determining the optimal delivery modality are known in the art and further contemplated herein. 
     Cell Penetrating Compounds (“CPCs”) 
     Numerous compounds are known to act as carriers for cargo such as nucleic acids and facilitate entry of the nucleic acid in a cell in an in vivo setting. Exemplary carriers are described in Dietz et al., Molecular &amp; Cellular Neuroscience, 27(2): 85-131 (2004) which is incorporated herein by reference. The prototypical CPCs derived from the Tat and antennepedia transcriptional regulators have been joined by a large number of new moieties. As an example, CPCs that are peptides can be relatively short (9-30 amino acids) polycationic peptides rich in argine and lysine, or membrane-interactive hydrophobic sequences. CPCs can be linked by recombinant DNA techniques or chemically coupled to peptides, oligonucleotides or nanocarriers, which then comprise the ‘cargo’ for the CPC. 
     Cell Targeting Ligands (“CTLs”) 
     Another strategy is to deliver oligonucleotides by use of a CTL that binds with high affinity to a cell surface receptor that is capable of undergoing efficient internalization. Potential ligands include antibodies, polypeptides derived from phage display libraries, and small organic molecules. Additional cell-targeting ligands are known in the art, or will be developed, and are contemplated for use with the invention described herein (for ASGPR—GalNAc conjugated siRNA and oligonucleotides, e.g., WO2012037254A1). Because various receptors are often preferentially expressed on particular cell types, this approach offers the possibility of improved selectivity for the oligonucleotide reagents. Exemplary receptor targets include, but are not limited to, lipoprotein receptors (such as those in the liver), integrins, receptor tyrosine kinases, and the G-protein coupled receptor (GPCR) superfamily. 
     Nanocarriers 
     A variety of supramolecular nanocarriers can be used to deliver nucleic acids. Exemplary nanocarriers include, but are not limited to liposomes, cationic polymer complexes and various polymeric. Complexation of nucleic acids with various polycations is another approach for intracellular delivery; this includes use of PEGlyated polycations, polyethyleneamine (PEI) complexes, cationic block co-polymers, and dendrimers. Several cationic nanocarriers, including PEI and polyamidoamine dendrimers help to release contents from endosomes. Other approaches include use of polymeric nanoparticles, polymer micelles, quantum dots and lipoplexes. 
     Additional nucleic acid delivery strategies are known in addition to the exemplary delivery strategies described herein. 
     In therapeutic and/or diagnostic applications, the compounds of the invention can be formulated for a variety of modes of administration, including systemic and topical or localized administration. Techniques and formulations generally may be found in Remington, The Science and Practice of Pharmacy, (20th ed. 2000). 
     Provided oligonucleotides, and compositions thereof, are effective over a wide dosage range. For example, in the treatment of adult humans, dosages from about 0.01 to about 1000 mg, from about 0.5 to about 100 mg, from about 1 to about 50 mg per day, and from about 5 to about 100 mg per day are examples of dosages that may be used. The exact dosage will depend upon the route of administration, the form in which the compound is administered, the subject to be treated, the body weight of the subject to be treated, and the preference and experience of the attending physician. 
     Pharmaceutically acceptable salts are generally well known to those of ordinary skill in the art, and may include, by way of example but not limitation, acetate, benzenesulfonate, besylate, benzoate, bicarbonate, bitartrate, bromide, calcium edetate, carnsylate, carbonate, citrate, edetate, edisylate, estolate, esylate, fumarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, iodide, isethionate, lactate, lactobionate, malate, maleate, mandelate, mesylate, mucate, napsylate, nitrate, pamoate (embonate), pantothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, subacetate, succinate, sulfate, tannate, tartrate, or teoclate. Other pharmaceutically acceptable salts may be found in, for example, Remington, The Science and Practice of Pharmacy (20th ed. 2000). Preferred pharmaceutically acceptable salts include, for example, acetate, benzoate, bromide, carbonate, citrate, gluconate, hydrobromide, hydrochloride, maleate, mesylate, napsylate, pamoate (embonate), phosphate, salicylate, succinate, sulfate, or tartrate. 
     Depending on the specific conditions being treated, such agents may be formulated into liquid or solid dosage forms and administered systemically or locally. The agents may be delivered, for example, in a timed- or sustained-low release form as is known to those skilled in the art. Techniques for formulation and administration may be found in Remington, The Science and Practice of Pharmacy (20th ed. 2000). Suitable routes may include oral, buccal, by inhalation spray, sublingual, rectal, transdermal, vaginal, transmucosal, nasal or intestinal administration; parenteral delivery, including intramuscular, subcutaneous, intramedullary injections, as well as intrathecal, direct intraventricular, intravenous, intra-articullar, intra-sternal, intra-synovial, intra-hepatic, intralesional, intracranial, intraperitoneal, intranasal, or intraocular injections or other modes of delivery. 
     For injection, the agents of the invention may be formulated and diluted in aqueous solutions, such as in physiologically compatible buffers such as Hank&#39;s solution, Ringer&#39;s solution, or physiological saline buffer. For such transmucosal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art. 
     Use of pharmaceutically acceptable inert carriers to formulate the compounds herein disclosed for the practice of the invention into dosages suitable for systemic administration is within the scope of the invention. With proper choice of carrier and suitable manufacturing practice, the compositions of the present invention, in particular, those formulated as solutions, may be administered parenterally, such as by intravenous injection. 
     The compounds can be formulated readily using pharmaceutically acceptable carriers well known in the art into dosages suitable for oral administration. Such carriers enable the compounds of the invention to be formulated as tablets, pills, capsules, liquids, gels, syrups, slurries, suspensions and the like, for oral ingestion by a subject (e.g., patient) to be treated. 
     For nasal or inhalation delivery, the agents of the invention may also be formulated by methods known to those of skill in the art, and may include, for example, but not limited to, examples of solubilizing, diluting, or dispersing substances such as, saline, preservatives, such as benzyl alcohol, absorption promoters, and fluorocarbons. 
     Pharmaceutical compositions suitable for use in the present invention include compositions wherein the active ingredients are contained in an effective amount to achieve its intended purpose. Determination of the effective amounts is well within the capability of those skilled in the art, especially in light of the detailed disclosure provided herein. 
     In addition to the active ingredients, these pharmaceutical compositions may contain suitable pharmaceutically acceptable carriers comprising excipients and auxiliaries which facilitate processing of the active compounds into preparations which can be used pharmaceutically. The preparations formulated for oral administration may be in the form of tablets, dragees, capsules, or solutions. 
     Pharmaceutical preparations for oral use can be obtained by combining the active compounds with solid excipients, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores. Suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethyl-cellulose, sodium carboxymethyl-cellulose (CMC), and/or polyvinylpyrrolidone (PVP: povidone). If desired, disintegrating agents may be added, such as the cross-linked polyvinylpyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate. 
     Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions may be used, which may optionally contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol (PEG), and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dye-stuffs or pigments may be added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses. 
     Pharmaceutical preparations that can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin, and a plasticizer, such as glycerol or sorbitol. The push-fit capsules can contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols (PEGs). In addition, stabilizers may be added. 
     Depending upon the particular condition, or disease state, to be treated or prevented, additional therapeutic agents, which are normally administered to treat or prevent that condition, may be administered together with the inhibitors of this invention. For example, chemotherapeutic agents or other anti-proliferative agents may be combined with the inhibitors of this invention to treat proliferative diseases and cancer. Examples of known chemotherapeutic agents include, but are not limited to, adriamycin, dexamethasone, vincristine, cyclophosphamide, fluorouracil, topotecan, taxol, interferons, and platinum derivatives. 
     Other examples of agents the non-racemic pro-oligonucleotide of this invention may also be combined with include, without limitation, anti-inflammatory agents such as corticosteroids, TNF blockers, IL-1 RA, azathioprine, cyclophosphamide, and sulfasalazine; immunomodulatory and immunosuppressive agents such as cyclosporin, tacrolimus, rapamycin, mycophenolate mofetil, interferons, corticosteroids, cyclophophamide, azathioprine, and sulfasalazine; neurotrophic factors such as acetylcholinesterase inhibitors, MAO inhibitors, interferons, anti-convulsants, ion channel blockers, riluzole, and anti-Parkinsonian agents; agents for treating cardiovascular disease such as beta-blockers, ACE inhibitors, diuretics, nitrates, calcium channel blockers, and statins; agents for treating liver disease such as corticosteroids, cholestyramine, interferons, and anti-viral agents; agents for treating blood disorders such as corticosteroids, anti-leukemic agents, and growth factors; agents for treating diabetes such as insulin, insulin analogues, alpha glucosidase inhibitors, biguanides, and insulin sensitizers; and agents for treating immunodeficiency disorders such as gamma globulin. 
     These additional agents may be administered separately, as part of a multiple dosage regimen, from provided chirally controlled oligonucleotides, and composition thereof. Alternatively, these agents may be part of a single dosage form, mixed together with the provided chirally controlled oligonucleotides, and composition thereof, in a single composition. 
     The examples and preparations provided below further illustrate and exemplify provided oligonucleotides and compositions thereof, and methods of preparing the same. It is to be understood that the scope of the present invention is not limited in any way by the scope of the following examples and preparations. 
     The function and advantage of these and other embodiments of the present invention will be more fully understood from the examples described below. The following examples are intended to illustrate the benefits of the present invention, but do not exemplify the full scope of the invention. 
     Method of Analyzing Oligonucleotide Compositions 
     In some embodiments, the present invention provides methods for analyzing oligonucleotide compositions. In some embodiments, a provided method detects, determines, and/or quantifies, for example, the identity, purity, quantity and/or quality of one or more oligonucleotides. In some embodiments, the present invention provides a method comprising steps of: 
     a) performing a first analysis of a first composition, which first composition comprises a plurality of different types of an oligonucleotide; and 
     b) comparing the performed first analysis with a second analysis, under comparable conditions as the first analysis, of a second composition, which second composition is a chirally controlled composition of the oligonucleotide, where differences between the first and second analyses reflect differences in presence or level of at least one type of the oligonucleotide in the first as compared with the second composition. 
     In some embodiments, a second composition contains only a single type of the oligonucleotide. In some embodiments, a second composition contains more than one types of the oligonucleotide. In some embodiments, a provided method is used for quality control of oligonucleotide compositions, for example, as illustrated in Examples 17 and 48. As will be appreciated by those skilled in the art, the presented data in Example 48 confirm that the depicted analyses comparing the compositions show that the random oligonucleotide composition (the first composition), as prepared by non-chiral controlled oligonucleotide synthesis, comprises extremely low level of certain oligonucleotide types, such as the full Rp or Sp type of the chirally controlled composition (the second composition). 
     EXEMPLIFICATION 
     The foregoing has been a description of certain non-limiting embodiments of the invention. Accordingly, it is to be understood that the embodiments of the invention herein described are merely illustrative of the application of the principles of the invention. Reference herein to details of the illustrated embodiments is not intended to limit the scope of the claims. 
     General Description of Oligonucleotide Synthesis 
     As described above and herein, in some embodiments, the present invention provides oligonucleotides and the methods of preparing the same. In some embodiments, the synthesis of oligonucleotide is performed using solid support. In some embodiments, the synthesis of oligonucleotide is performed in solution. In some embodiments, the synthesis of oligonucleotide comprises steps using solid support and steps in solution phase. In some embodiments, the steps using solid support are performed on an oligonucleotide synthesizer. 
     Preparation of the Solid Support: The support (Highly Cross-linked Polystyrene (HCP) or Controlled Pore Glass (CPG)) was used to load the first 5′-O-DMTr protected nucleoside contained in the oligonucleotide sequence. In some embodiments, an oxalyl group was used to link the 3′-OH group of the first nucleoside to an amine group on the solid support, as described in Alul et al., Oxalyl-CPG: a labile support for synthesis of sensitive oligonucleotide derivatives,  Nucleic Acids Research  1991, 19(7), 1527. In some embodiments, the standard succinyl group was used as the linker. 
     Detritylation of Nucleotide on the Solid Support: A solution of 3% TCA (trichloroacetic acid) in dichloromethane (DCM, CH 2 Cl 2 ) was delivered to the column containing the support which was installed on a synthesizer to de-block the 5′-O-DMTr. 
     CMPT-Mediated Chiral Phosphoramidite Coupling: After washing of the solid support with anhydrous acetonitrile (ACN) and drying by reverse flush with dry argon, the free 5′-OH was coupled with the next nucleotide in the oligonucleotide sequence, growing from the 3′ to the 5′ end. This entailed co-delivery to the solid support of a solution of the chiral phosphoramidite together with CMPT (below), which resulted in highly efficient coupling with excellent diastereomeric excess (typically &gt;99%) to give a chiral phosphite diester product, as described by Wada et al.  J. Am. Chem. Soc.,  2008, 130, 16031-1603 ; Angew. Chem. Intern. Ed.  2009, 48, 496-499. The solvent was normally acetonitrile (ACN), but other solvents could also be used. 
                         
Chiral Phosphoramidites Employed in these Studies:
 
                                                                                                                                                                                                                               
Additional Phosphoramidites:
 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Capping: In some embodiments, the capping step was carried out in two steps. In some embodiments, the above-mentioned two capping steps were combined into one and performed in one-pot. Unless otherwise specified, the two-step capping was used in the synthesis of the examples described herein. 
     For the two-step capping, after washing of the solid support with anhydrous ACN and drying by reverse flush with dry argon, the ‘Cap A’ reagent, typically comprising 5% Pac 2 O solution in 2,6-lutidine/THF—1:4 (v/v) was first introduced to the solid support. Without the intention to be limited by theory, Cap A was sufficiently reactive to effectively cap (acylate) the free amine of the chiral auxiliary. In some embodiments, such protection prevents rearrangement/decomposition of the intermediate phosphite. Immediately following this treatment, a 1:1 mixture of ‘Cap A’ and ‘Cap B’ reagents was sent to the column containing the solid support. ‘Cap B’ was a solution of 16% N-methylimidazole (NMI) in THF which, when mixed with ‘Cap A’ effected capping of, for example, the unreacted 5′-OH oligonucleotide bound to the solid support. Not wishing to be limited by theory, the capping step was extremely important in that, without capping of the amino group of the chiral auxiliary the following sulfurization step within the cycle induced the cleavage of the internucleotidic linkage and decomposition of the oligonucleotide, for instance, due to an undesired intermolecular nucleophilic attack of the free amine of the auxiliary on the phosphorous atom. Moreover, capping of the 5′-OH groups of the run-off uncoupled solid-supported oligonucleotide shortmers is crucial in regards of obtaining high purity of the crude full-length oligonucleotide, avoiding low yields due to accumulation of extended failed sequences and tedious and difficult, if not impossible final purifications. 
     Sulfurization: After washing of the solid support with anhydrous ACN and drying by reverse flush with dry argon, the resulting capped phosphite triester intermediate was subject to oxidative sulfurization. A solution of sulfurizing reagent was prepared by mixing the sulfurizing reagent, for example, an alkyl thiosulfonate derivative (300 mM), with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) (100 mM) in ACN. This was then delivered to the column containing the solid support and allowed to react for a certain amount of time (usually 5-60 min). In some embodiments, the sulfurizing thiosulfonate reagent was used as a 300 mM solution in anhydrous ACN, without addition of BSTFA. In some embodiments, the reagent solutions with and without BSTFA produced similar results. 
     Oxidation: In some embodiments, oxidation to phosphodiester was performed instead of the sulfurization step. Oxidation was achieved by delivering a solution of 0.02 M I 2  in a THF/pyridine/water (70:20:10—v/v/v) co-solvent system, forming a non-chiral phosphodiester linkage. 
     5′-Deblocking of the DMTr group: DMTr removal was effected using 3% TCA in DCM. After de-blocking, the cycle can then be carried forward for further iterative rounds of coupling, capping, sulfurization/oxidation (either sulfurization with the same sulfurizing agent, or sulfurization with a different sulfurizing agent, or oxidation instead of sulfurization, for each of the next cycles) and de-blocking. Alternatively, if the pre-designed length was reached, the oligonucleotide was subject to cycle exit and, optionally, post-synthetic treatment. In some embodiments, the oligonucleotide was removed before de-blocking of the terminal 5′-O-DMTr group. 
     Spontaneous Removal of Chiral Auxiliary: The spontaneous removal of chiral auxiliary was achieved during sulfurization, oxidation, de-blocking of the DMTr group, or the combination thereof. No specific step was needed to remove the chiral auxiliary. 
     Cleavage and Deprotection: The desired length oligonucleotide was removed from its solid support by cleaving the corresponding linker (oxalyl or succinyl linked HCP or CPG) whilst at the same time, the protecting groups on the oligonucleotide were deprotected. In some embodiments, the solid-supported oligonucleotide was first treated with an anhydrous 1 M solution of 1,5-diazabicyclo(4.3.0)non-5-ene (DBN) or 1,8-diazabicycloundec-7-ene (DBU) in dry ACN-trimethylsilyl chloride—16:1 (v/v) for 10 min at r.t. and then washed with dry ACN, as described by Reese and Yan,  J. Chem. Soc., Perkin Trans.  1, 2002, 2619. In some embodiments, the material was treated with solutions of dry propylamine in dry pyridine (typically in a 1:4 ratio) for a period of 18 h at r.t. or at 60° C. for 2 h. In some embodiments, either condition afforded the crude compound with similar quality. In some embodiments, the crude oligonucleotides were cleaved from the support and deprotected by treatment with 28% aqueous ammonia for a period of 18 h at r.t. or at 60° C. for 5 h. In some embodiments, either condition afforded the crude compound with similar quality. The solvents were then evaporated and the residue was treated with ˜pH 1.5 aqueous solution (the pH may be altered if desired) with 0-50% DMSO, and the crude product was subject to analysis by a combination of HPLC and UPLC/MS. The product was purified by one of or a combination of reverse phase HPLC (RP-HPLC), normal phase HPLC, ion exchange HPLC (IE-HPLC) or size exclusion chromatography. In some embodiments, the oligonucleotide was removed from the solid support, deprotected and purified before de-blocking of the DMTr group. 
     Example 1: Synthesis of Sulfurizing Reagents and Synthesis of Phosphoramidites 
     In some embodiments, the present invention provides sulfurizing reagents, and methods of making the same. In some embodiments, the provided sulfurizing reagents were used in the synthesis of oligonucleotides described in the present application. Exemplary sulfurizing reagents and their synthesis are illustrated in Scheme E-1 
                                                                                                                                                                                                             
Synthesis of Compound 5
 
     
       
         
         
             
             
         
       
     
     Compound 2: A solution of (Z)-but-2-ene-1,4-diol (0.93 ml, 11.3 mmol) and triethylamine (3.3 ml, 24 mmol) in dichloromethane (DCM, 50 mL) was added in a dropwise fashion to a stirring ice cold solution of methanesulfonyl chloride (1.9 ml, 24 mmol) in DCM (50 mL). After stirring for 0.5 h at r.t. the mixture was poured onto ice and extracted. The organic layer was collected, dried (MgSO 4 ) and filtered. After removal of solvent, 2.66 g compound 2 was obtained (96%), which was judged by NMR to be sufficiently pure for direct use in the next step of the reaction.  1 H NMR (399 MHz, CDCl 3 ) δ 5.94 (ddd, J=5.4, 4.1, 1.3 Hz, 2H), 4.83 (dd, J=4.1, 1.3 Hz, 4H), 3.04 (s, 6H);  13 C NMR 128.34, 64.38, 38.27; MS (ESI +ve): calc (M+NH 4 ) + : 262.04, found: 262.05; R f =0.3 (1:1 EtOAc/hexane). 
     Compound 3: A solution of sodium methanesulfonothioate (1.51 g, 11.3 mmol) in MeOH (20 ml) was treated with neat (Z)-but-2-ene-1,4-diyl dimethanesulfonate (1.25 g, 5.12 mmol) at room temperature. After 5 min, precipitation was observed to occur. After 36 h, the mixture was partitioned between water and DCM. The organic layer was separated, dried (MgSO 4 ), and filtered. Removal of solvent afforded colorless oil. Column chromatography (ISCO) gave pure compound 3 (0.89 g, 63%) as a colorless oil.  1 H NMR (399 MHz, CDCl 3 ) δ 5.84 (ddd, J=6.6, 5.1, 1.5 Hz, 2H), 3.92 (dd, J=5.1, 1.5 HZ, 4H), 3.33 (s, 6H);  13 C NMR 128.1, 51.47, 33.13; MS (ESI +ve): calc (M+NH 4 ) + : 294.00, found: 294.04; R f =0.4 (1:1 EtOAc/hexane). 
     Compound 4: Under argon atmosphere, morpholine (10 g, 115 mmol) was added to ethylene sulfide (15 g, 250 mmol) in a round bottom flask. The reaction was stirred for 7 hrs and was directly loaded on to a silica gel column. The column was washed with DCM first and then 2% MeOH/DCM was used to obtain compound 4 (15.3 g, 91%) as colorless oil.  1 H NMR (399 MHz, CDCl 3 ) δ 3.67-3.59 (m, 4H), 2.63-2.52 (m, 2H), 2.51-2.45 (m, 2H), 2.44-2.34 (m, 4H); MS (ESI +ve): calc (M+H) + =148.07, found: 148.1. 
     Compound 5: A DCM solution (1 mL) of 2-morpholinoethanethiol (0.21 g, 1.44 mmol) was added dropwise via syringe to a stirring solution compound 3 (0.40 g, 1.44 mmol) in DCM (10 mL) at room temperature. Immediately after addition, the reaction was checked by TLC, showing rapid formation of the product and some dimer. After 0.5 h, the mixture was partitioned by addition of water. Upon extraction, the organic layer was separated, dried (MgSO 4 ) and filtered. After removal of solvent in vacuo, column chromatography gave compound 5 (0.29 g, 58%) as colorless oil.  1 H NMR (399 MHz, CDCl 3 ) δ 5.78 (m, 2H), 3.92 (d, J=7.3 Hz, 2H), 3.70 (t, J=4.7 Hz, 4H), 3.46 (d, J=5.5 Hz, 2H), 3.31 (s, 3H), 2.84 (dd, J=7.8, 6.7 Hz, 2H), 2.66 (dd, J=7.8, 6.7, 2H), 2.48 (t, J=4.6 Hz, 4H);  13 C NMR 130.35, 126.27, 66.97, 58.20, 53.67, 51.52, 36.22, 35.16, 33.67; MS (ESI +ve): calc (M+H) + : 344.05, found: 344.06; R f =0.3 (EtOAc). 
     
       
         
         
             
             
         
       
     
     Compound 5b: A DCM solution (1 mL) of compound 4b (395 mg, 1.085 mmol) was added dropwise via syringe to a stirring DCM (15 mL) solution compound 3 (300 mg, 1.085 mmol) at r.t. After 1 h, the resulting solution was partitioned by addition of water. Upon extraction, the organic layer was separated, dried (MgSO 4 ) and filtered. After removal of the solvent in vacuo, column chromatography gave compound 5b as a colorless oil (0.35 g, 58%).  1 H NMR (399 MHz, CDCl 3 ) δ 5.83-5.70 (m, 2H), 5.35-5.21 (dt, J=26.0, 9.3 Hz, 2H), 5.16-5.07 (m, 1H), 4.59-4.54 (d, J=9.5 Hz, 1H), 4.29-4.23 (m, 1H), 4.23-4.18 (m, 1H), 3.99-3.88 (dd, J=6.7, 1.2 Hz, 2H), 3.80-3.72 (ddd, J=10.1, 4.6, 2.6 Hz, 1H), 3.64-3.56 (m, 1H), 3.50-3.43 (m, 1H), 3.31 (s, 3H), 2.09 (s, 3H), 2.03 (s, 6H), 2.00 (s, 3H);  13 C NMR (100 MHz, CDCl 3 ) δ 170.68, 170.30, 169.51, 169.30, 129.43, 127.14, 87.73, 76.49, 73.89, 69.16, 67.99, 61.99, 51.64, 35.89, 33.58, 20.95, 20.80, 20.74, 20.71; MS (ESI +ve): calc (M+NH 4 ) + : 578.07, found: 577.96; R f =0.5 (1:1 EtOAc/hexane). 
     Synthesis of Compound 7 
     
       
         
         
             
             
         
       
     
     Compound 6: An ice cold solution of (Z)-but-2-ene-1,4-diol (0.93 ml, 11.3 mmol) and triethylamine (1.6 mL, 11.5 mmol) in DCM (50 ml) was treated dropwise via syringe with pivaloyl chloride (1.4 ml, 11.4 mmol) over 2 min. After 1 h, TLC showed good reaction results. The resulting mixture was partitioned by addition of water. Upon extraction, the organic layer was separated, dried (MgSO 4 ), and concentrated in vacuo. This crude compound was found: by TLC (Rf=0.6, 1:1 EtOAc/hexane) to contain no starting diol and was used crude to prepare the mesylate. The crude material was taken up in DCM (50 ml) containing triethylamine (1.7 mL, 12 mmol) and cooled on an ice bath. Methanesulfonyl chloride (0.98 ml, 12.66 mmol) was added dropwise via syringe over 2 min. TLC immediately after addition indicated complete consumption of starting material. The resulting mixture was partitioned by addition of water. Upon extraction, the organic layer was separated, dried (MgSO 4 ), filtered and concentrated in vacuo. Column chromatography gave pure compound 6, 1.48 g, 52%, as a colorless oil.  1 H NMR (399 MHz, CDCl 3 ) δ 5.89-5.75 (m, 2H), 4.89-4.84 (d, J=5.7 Hz, 2H), 4.68-4.63 (d, J=5.9 Hz, 2H), 3.03 (s, 3H), 1.19 (s, 9H);  13 C NMR (100 MHz, CDCl 3 ) δ 178.28, 130.61, 126.11, 65.08, 59.65, 38.84, 38.21, 27.25; MS (ESI +ve): calc (M+NH 4 ) + : 268.12, found: 268.20; R f =0.3 (20% EtOAc/hexane). 
     Compound 7: A MeOH (10 ml) solution of sodium methanesulfonothioate (0.63 g, 4.70 mmol) and (Z)-4-(methylsulfonyloxy)but-2-enyl pivalate (1.00 g, 4.00 mmol) was stirred at r.t. for 18 h with formation of a white precipitate (after 10 min). The resulting mixture was partitioned by addition of water and DCM. Upon extraction into DCM, the organic layer was separated, dried (MgSO4), filtered and concentrated in vacuo. Column chromatography gave compound 7, 0.83 g, 78% as a colorless oil.  1 H NMR (399 MHz, CDCl 3 ) δ 5.82-5.73 (m, 2H), 4.73-4.66 (m, 2H), 3.95-3.87 (m, 2H), 3.32 (s, 3H), 1.19 (s, 9H);  13 C NMR (100 MHz, CDCl 3 ) δ 178.35, 129.37, 127.32, 59.50, 51.44, 38.84, 33.61, 27.28; MS (ESI +ve): calc (M+NH 4 ) + : 284.10, found: 284.19; R f =0.4 (20% EtOAc/hexane). 
     Synthesis of Compound 9 
     
       
         
         
             
             
         
       
     
     Compound 9: Pivaloyl chloride (0.60 g, 5.0 mmol) was added in a dropwise fashion to a stirring solution of S-2-hydroxyethyl methanesulfonothioate (0.65 g, 4.16 mmol) in DCM (20 ml). After 2 h at r.t. the resulting mixture with white precipitate was partitioned with water. The organic layer was separated, dried (Na 2 SO 4 ), filtered concentrated to an oil. Column chromatography gave compound 9 as a colorless oil (0.45 g, 45%).  1 H NMR (399 MHz, CDCl 3 ) δ 4.39-4.34 (t, J=6.3 Hz, 2H), 3.44-3.39 (t, J=6.3 Hz, 2H), 3.36 (s, 3H), 1.20 (s, 9H);  13 C NMR (100 MHz, CDCl 3 ) δ 62.10, 51.11, 38.96, 35.19, 27.24; MS (ESI +ve): calc (M+NH 4 ) + : 158.08, found: 158.04; R f =0.3 (20% EtOAc/hexane). 
     Synthesis of Compound 12 
     
       
         
         
             
             
         
       
     
     Compound 11: Pivaloyl chloride (4.96 ml, 40.3 mmol) was added dropwise via syringe to an ice cold DCM solution (50 mL) of 2-(hydroxymethyl)phenol (5 g, 40.3 mmol) and triethylamine (5.61 ml, 40.3 mmol). An ice-cold solution of the crude pivalate ester was treated with triethylamine (6.74 ml, 48.4 mmol) and 50 mL DCM. Methanesulfonyl chloride (3.43 ml, 44.3 mmol) was then added slowly (5 min) via syringe and the resulting mixture was warmed to r.t. The mixture was poured onto ice and the organic layer was separated then washed with sat NaHCO 3  (aq), dried (MgSO 4 ), filtered and concentrated to afford 10.5 g crude pale yellow oil. Column chromatography (ISCO) gave pure 11 5.45 g, 47%.  1 H NMR (399 MHz, CDCl 3 ) δ 7.53-7.46 (dd, 7.7, 1.8 Hz, 1H), 7.46-7.40 (dt, 7.7, 1.8 Hz, 1H), 7.32-7.24 (t, 7.7 Hz, 1H), 7.13-7.06 (d, 7.7 Hz, 1H), 5.21 (s, 2H), 2.79 (s, 3H), 1.40 (s, 9H);  13 C NMR (100 MHz, CDCl 3 ) δ 177.05, 150.06, 131.18, 131.07, 126.35, 125.94, 123.21, 66.88, 39.48, 38.82, 27.30, 27.26. MS (ESI +ve): calc (M+NH 4 ) + : 304.12, found: 303.99; R f =0.4 (20% EtOAc/hexane). 
     Compound 12: A MeOH (20 mL) solution of sodium methanesulfonothioate (0.825 g, 6.15 mmol) was treated with 2-((methylsulfonyloxy)methyl)phenyl pivalate (1.76 g, 6.15 mmol) at r.t. and left to stir for 18 h. The mixture was partitioned between water and DCM. The organic layer was separated, dried (MgSO 4 ), filtered and concentrated to afford a colorless oil. Column chromatography gave pure compound 12 as a pale colorless oil, 0.754 g, 41%.  1 H NMR (399 MHz, CDCl 3 ) δ 7.48-7.44 (dd, J=7.7, 1.7 Hz, 1H), 7.39-7.34 (td, J=7.8, 1.7 Hz, 1H), 7.25-7.20 (td, J=7.6, 1.2 Hz, 1H), 7.10-7.06 (dd, J=8.2, 1.2 Hz, 1H), 4.29 (s, 2H), 2.90 (s, 3H), 1.39 (s, 9H);  13 C NMR (100 MHz, CDCl 3 ) δ 176.69, 149.59, 131.17, 129.85, 127.41, 126.18, 123.40, 51.43, 39.47, 36.01, 27.30; MS (ESI +ve): calc (M+NH 4 ) + : 320.10, found: 320.09; R f =0.4 (20% EtOAc/hexane). 
     Synthesis of Compound 14 
     
       
         
         
             
             
         
       
     
     Compound 14: Chloromethyl pivalate (0.478 ml, 3.32 mmol) was added to a stirring mixture of sodium iodide (0.050 g, 0.33 mmol) and sodium methanesulfonothioate (0.445 g, 3.32 mmol) in acetone (7 ml) at r.t. After 24 h, TLC showed good conversion to product. The solvent was removed, and the residue was partitioned between water and DCM. The organic layer was separated and dried (MgSO 4 ), filtered and concentrated to afford a colorless oil. Column chromatography gave pure 14 as a slightly pink solid, 0.41 g, 55%.  1 H NMR (399 MHz, CDCl 3 ) δ 5.67 (s, 2H), 3.39 (s, 3H), 1.24 (s, 9H);  13 C NMR (100 MHz, CDCl 3 ) δ 177.35, 67.84, 52.20, 38.93, 27.05; R f =0.5 (20% EtOAc/hexane). 
     Synthesis of Compound 16 
     
       
         
         
             
             
         
       
     
     Compound 16: Prepared from 15 and NaMTS as described previously in U.S. Pat. No. 3,484,473.  1 H NMR (399 MHz, CDCl 3 ) δ 4.86 (s, 2H), 3.45 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 52.15, 41.50. 
     Synthesis of Compounds 18 and 19 
     
       
         
         
             
             
         
       
     
     Compound 18: Prepared from 17 and NaMTS as described previously: Chem. Pharm. Bull. Vol. 12(11) p. 1271, 1964.  1 H NMR (399 MHz, CDCl 3 ) δ 3.55 (s, 4H), 3.40 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 50.67, 35.96. 
     Compound 19: A DCM solution (1 mL) of 2-morpholinoethanethiol (0.17 g, 1.2 mmol) was added dropwise via syringe to a stirring solution of compound 18 (300 mg, 1.2 mmol) in DCM (10 mL) at r.t. Immediately after addition, TLC showed rapid formation of product and some dimer. After 0.5 h, the mixture was partitioned by addition of NaHCO 3  solution. Upon extraction, the organic layer was separated, dried (MgSO 4 ), filtered and concentrated in vacuo. Column chromatography gave pure 19 (0.20 g, 53%) as a colorless oil.  1 H NMR (399 MHz, CDCl 3 ) δ 3.73-3.67 (t, J=4.7 Hz, 4H), 3.51-3.46 (m, 2H), 3.35 (s, 3H), 3.07-3.01 (m, 2H), 2.88-2.83 (m, 2H), 2.69-2.63 (m, 2H), 2.52-2.43 (t, J=4.6 Hz, 4H);  13 C NMR (100 MHz, CDCl 3 ) δ 66.96, 57.91, 53.58, 50.79, 37.66, 36.10, 35.52; MS (ESI +ve): calc (M+H) + : 318.03, found: 318.04; R f =0.3 (EtOAc). 
     Synthesis of Compound 22 
     
       
         
         
             
             
         
       
     
     Compound 21: Compound 20 was converted to compound 21 by a procedure analogous to that described for compound 11.  1 H NMR (399 MHz, CDCl 3 ) δ 7.45-7.36 (m, 4H), 5.37 (s, 2H), 5.21 (s, 2H), 2.93 (s, 3H), 1.21 (s, 9H);  13 C NMR (100 MHz, CDCl 3 ) δ 178.20, 135.65, 131.92, 130.48, 129.98, 129.78, 128.88, 69.05, 63.39, 38.94, 38.36, 27.27; MS (ESI +ve): calc (M+NH 4 ) + : 318.24, found: 318.14; R f =0.4 (20% EtOAc/hexane). 
     Compound 22: Compound 21 was converted to compound 22 by a procedure analogous to that described for compound 12.  1 H NMR (399 MHz, CDCl 3 ) δ 7.46-7.32, (m, 4H), 5.21 (s, 2H), 4.50 (s, 2H), 3.03 (s, 3H), 1.21 (s, 9H);  13 C NMR (100 MHz, CDCl 3 ) δ 178.24, 135.10, 133.15, 130.93, 130.32, 129.05, 129.00, 63.61, 51.07, 38.97, 38.03, 27.30; MS (ESI +ve): calc (M+NH 4 ) + : 334.11, found: 334.13; R f =0.4 (20% EtOAc/hexane). 
     Synthesis of Compound 25 
     
       
         
         
             
             
         
       
     
     Compound 23: Compound 23 is prepared according to a literature method (Journal of Medicinal Chemistry, 50(23), 5568-5570, 2007). 
     Compound 24: An ice-cold pyridine solution (10 mL) of compound 23 (1 mmol) is treated successively, in a dropwise fashion with acetyl chloride (1 mmol), then after 5 min with MsCl (1.1 mmol). The solution is warmed to room temperature then the solvent is removed. The residue is dissolved in EtOAc, washed with water, dried (MgSO 4 ), filtered and concentrated in vacuo. Purification by column chromatography affords pure compound 24. 
     Compound 25: Compound 24 is converted to compound 25 by a procedure analogous to that described for compound 12. 
     Synthesis of Compound 27 
     
       
         
         
             
             
         
       
     
     Compound 27: Compound 26 was converted to compound 27 by a procedure analogous to that described for compound 14.  1 H NMR (399 MHz, CDCl 3 ) δ 3.97 (s, 2H), 3.79 (s, 3H), 3.48 (s, 3H);  13 C NMR (100 MHz, CDCl 3 ) δ 168.84, 53.35, 51.53, 37.83; MS (ESI +ve): calc (M+NH 4 ) + : 202.02, found: 201.96; R f =0.2 (20% EtOAc/hexane). 
     Synthesis of Compound 29 
     
       
         
         
             
             
         
       
     
     Compound 29: Compound 28 was converted to compound 29 by a procedure analogous to that described for compound 14.  1 H NMR (399 MHz, CDCl 3 ) δ 3.72 (s, 3H), 3.39 (t, J=6.8 Hz, 2H), 3.34 (s, 3H), 2.85 (t, J=6.8 Hz, 2H);  13 C NMR (100 MHz, CDCl 3 ) δ 171.53, 52.29, 50.66, 34.51, 31.20; MS (ESI +ve): calc (M+NH 4 ) + : 216.10, found: 216.04; R f =0.2 (20% EtOAc/hexane). 
     Synthesis of Compound 31 
     
       
         
         
             
             
         
       
     
     Compound 30: Compound 30 is prepared according to a literature method (Tetrahedron, 42(2), 601-607; 1986). 
     Compound 31: Compound 31 is prepared from compound 30 according to a patent procedure (US 20090181444). 
     Synthesis of Compound 33 
     
       
         
         
             
             
         
       
     
     Compound 33: Compound 33 is prepared from compound 32 according to a patent procedure (US 20090181444). 
     Synthesis of Compound 38 
     
       
         
         
             
             
         
       
     
     Compound 36: An ice-cold DCM (20 mL) solution of compound 34 (1 mmol) is treated with NEt 3  (1 mmol) followed by the dropwise addition of TMS-Cl (1.1 mmol). After 1 h, the solution is washed with water, dried (MgSO 4 ), filtered and concentrated in vacuo. The crude TMS protected material is re-dissolved in THF (10 mL), whereon PPh 3  (1.2 mmol), compound 35 (1.2 mmol), then DEAD (1.2 mmol, dropwise) are added in succession. After stirring at room temperature for 18 h, the solvent is removed under vacuum, the residue is re-dissolved in DCM, the solution of which is washed with water, dried (MgSO 4 ), filtered and concentrated in vacuo. Purification by column chromatography affords pure compound 36. 
     Compound 37: A THF (10 mL) solution of compound 36 (0.5 mmol) is treated with TBAF (1 mmol of a 1M solution in THF), with monitoring by TLC. On completion of TMS cleavage, the solvent is removed under vacuum, and the residue is re-dissolved in DCM, the solution of which is washed with water, dried (MgSO 4 ), filtered and reduced in vacuo. The crude alcohol is re-dissolved in pyridine (5 mL), and TsCl (0.55 mmol) is added. After 18 h at room temperature, the solvent is removed, and the residue is re-dissolved in DCM, the solution of which is washed with water, dried (MgSO 4 ), filtered and reduced in vacuo. Purification by column chromatography affords pure compound 37. 
     Compound 38: Compound 37 is converted to compound 38 by a procedure analogous to that described for compound 12. 
     Synthesis of Compound 41 
     
       
         
         
             
             
         
       
     
     Compound 40: An ice-cold DCM (20 mL) solution of compound 39 (1 mmol) is treated with NEt 3  (1 mmol) followed by the dropwise addition of TMS-Cl (1.1 mmol). After 1 h, the solution is washed with water, dried (MgSO 4 ), filtered and concentrated in vacuo. The crude TMS protected material is re-dissolved in THF (10 mL), whereon PPh 3  (1.2 mmol), potassiump-toluenethiosulfonate (KTTS, 1.2 mmol), anhydrous ZnCl 2  (1 mmol) then DEAD (1.2 mmol, dropwise) are added in succession. After stirring at r.t. for 18 h, the solvent is removed under vacuum, and the residue is re-dissolved in DCM, the solution of which is washed with water, dried (MgSO 4 ), filtered and concentrated in vacuo. Purification by column chromatography affords pure compound 40. 
     Compound 41: A THF (10 mL) solution of compound 40 (0.5 mmol) is treated with TBAF (1 mmol of a 1M solution in THF), with monitoring by TLC. On completion of TMS cleavage, the solvent is removed under vacuum, and the residue is re-dissolved in DCM, the solution of which is washed with water, dried (MgSO 4 ), filtered and concentrated in vacuo. The crude alcohol is re-dissolved in THF (10 mL), whereon PPh 3  (1.2 mmol), compound 35 (1.2 mmol), then DEAD (1.2 mmol, dropwise) are added in succession. After stirring at r.t. for 18 h, the solvent is removed under vacuum, and the residue is re-dissolved in DCM, the solution of which is washed with water, dried (MgSO 4 ), filtered and concentrated in vacuo. Purification by column chromatography affords pure compound 41. 
     Synthesis of Compound 43 
     
       
         
         
             
             
         
       
     
     Compound 43: Compound 42 is converted to compound 43 by a procedure analogous to that described for compound 14. 
     Synthesis of Compound 45a, 45b, 47a and 47b 
     
       
         
         
             
             
         
       
     
     Compound 45a: A mixture of 4-(2-chloroethyl)morpholine hydrochloride (compound 44) (50 g, 269 mmol), sodium iodide (4.03 g, 26.9 mmol) and potassium 4-methylbenzenesulfonothioate (73.0 g, 322 mmol) was stirred in MeOH (200 ml) and heated at 60° C. over 72 h. The pale yellow mixture was cooled and diluted with 200 mL water, stirred for 0.5 h then the white solid was collected by filtration, washed with water (100 mL), IPA (200 mL), EtOAc (200 mL) and ether (200 mL). Dried mass=68 g. This microcrystalline powder was recrystallized from 90° C. water (200 mL) and the crystalline mass was collected by filtration after standing at r.t. overnight (64 g, 71%).  1 H NMR (500 MHz, CDCl 3 ) δ 7.83 (d, J=8.4 Hz, 2H), 7.36 (d, J=8.4 Hz, 2H), 3.67 (t, J=4.7 Hz, 4H), 3.16 (t, J=6.9 Hz, 2H), 2.64 (t, J=6.9 Hz, 2H), 2.47 (s, 3H), 2.41 (t, J=4.4 Hz, 4H);  13 C NMR (100 MHz, CDCl 3 ) δ 144.82, 141.99, 129.95, 127.14, 66.85, 56.54, 53.18, 33.44, 21.78; MS (ESI +ve): calc (M+H) + : 302.08, found: 302.22. 
     Compound 45b: Replacing potassium 4-methylbenzenesulfonothioate with potassium 4-chlorobenzenesulfonothioate compound 45b was synthesized by a method analogous to that described for compound 45a. (ESI +ve): calc (M+H) + : 324.03, 322.04, found: 324.22, 322.20 ( 37 Cl,  35 Cl isotope pattern). 
     Compound 47a: Replacing 4-(2-chloroethyl)morpholine hydrochloride with 4-(2-bromooethyl)-N-methylpiperazine hydrobromide (compound 46), compound 47a was synthesized by a method analogous to that described for compound 45a. MS (ESI +ve): calc (M+H) + : 315.12, found: 315.07. 
     Compound 47b: Replacing 4-(2-chloroethyl)morpholine hydrochloride with 4-(2-bromooethyl)-N-methylpiperazine hydrobromide and potassium 4-methylbenzenesulfonothioate with potassium 4-chlorobenzenesulfonothioate compound 47b is synthesized by a method analogous to that described for compound 45a. 
     Synthesis of Compound 50 
     
       
         
         
             
             
         
       
     
     Compound 49: A mixture of compound 48, (500 mg, 1.894 mmol) and sodium methanesulfonothioate (534 mg, 3.98 mmol) was dissolved in acetone (10 ml) and stirred at r.t. for 4 h. TLC indicated a complete reaction. The solvent was removed then the mixture was partitioned by addition of water and DCM. Upon extraction, the organic layer was separated then dried (MgSO 4 ), filtered and concentrated in vacuo. Column chromatography afforded the pure product as a colorless solid (0.60 g, 97%).  1 H NMR (399 MHz, CDCl 3 ) δ 7.47 (dd, J=5.5, 3.5 Hz, 2H), 7.38 (dd, J=5.5, 3.5 Hz, 2H), 4.55 (s, 4H), 3.13 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 133.41, 131.75, 129.51, 51.02, 37.09; MS (ESI +ve): calc (M+NH 4 ) + : 344.01, found: 344.01; R f =0.5 (1:1 EtOAc/hexane). 
     Compound 50: A DCM solution (2 mL) compound 4, (180 mg, 1.225 mmol) was added dropwise via syringe to a stirring solution of compound 49 (400 mg, 1.225 mmol) in DCM (20 mL) at r.t. After 0.5 h, the mixture was partitioned by addition of NaHCO 3 . Upon extraction, the organic layer was separated then dried (MgSO 4 ), filtered and concentrated in vacuo. Column chromatography gave the product (170 mg, 35%) as a colorless oil.  1 H NMR (399 MHz, CDCl 3 ) δ 7.43-7.39 (m, 1H), 7.35-7.27 (m, 3H), 4.54 (s, 2H), 4.03 (s, 2H), 3.67 (t, J=4.6 Hz, 4H), 3.05 (s, 3H), 2.58-2.50 (m, 4H), 2.38 (t, J=4.6 Hz, 4H);  13 C NMR (100 MHz, CDCl 3 ) δ 136.09, 133.20, 131.87, 131.21, 128.86, 128.54, 66.95, 57.95, 53.52, 51.04, 40.81, 38.18, 35.82; MS (ESI +ve): calc (M+H): 394.06, found: 394.23; R f =0.5 (EtOAc). 
     Synthesis of Compound 55 
     
       
         
         
             
             
         
       
     
     Compound 54: Bromine (0.246 ml, 4.78 mmol) in DCM (50 mL) was added in a dropwise fashion over 0.5 h to a stirring mixture of sodium 4-nitrobenzenesulfinate (2 g, 9.56 mmol) and 2-hydroxyethyl disulfide (0.585 ml, 4.78 mmol) in DCM (50 mL) at r.t. After 2 h at r.t. the mixture was filtered to remove salt. The solvent was removed then column purification by column chromatography gave the product (2.1 g, 83%) as a colorless oil. Rf=0.4 (1:1 EA/hexane.  1 H NMR (399 MHz, CDCl 3 ) δ 8.41 (d, J=8.8 Hz, 2H), 8.13 (d, J=8.8 Hz, 2H), 3.89 (t, J=5.8 Hz, 2H), 3.23 (t, J=5.8 Hz, 2H), 2.02-1.94 (s, br, 1H);  13 C NMR (100 MHz, CDCl 3 ) δ 149.73, 128.40, 124.82, 60.94, 39.28. 
     Compound 55: Compound 51 (1 g, 3.07 mmol) in DCM (50 mL) was treated dropwise with oxalyl dichloride (0.527 ml, 6.15 mmol) and stirred at r.t. During the reaction a colorless homogeneous solution was formed. After 1 h at r.t. the solvent was removed under reduced pressure. The white residue (crude acid chloride, compound 52) was re-dissolved in DCM (20 mL) and added to a stirring solution of compound 54 (0.48 g, 3.1 mmol) in pyridine (20 mL). After 18 h, it was judged that the reaction was complete. The solvents were removed. The residue was re-dissolved in toluene and partitioned with water. The toluene extracts were collected, washed with brine, dried (MgSO 4 ), filtered and concentrated. Column chromatography afforded pure compound 55 (0.86 g, 60%) as a colorless solid.  1 H NMR (399 MHz, CDCl 3 ) δ 8.31 (d, J=8.9 Hz, 2H), 8.03 (d, J=8.9 Hz, 2H), 7.7 (d, J=7.5 Hz, 2H), 7.58 (d, J=7.5 Hz, 2H), 7.42 (t, J=7.5 Hz, 2H), 7.32 (dt, J=7.5, 1.2 Hz, 2H), 5.22 (s, br, 1H), 4.34 (s, br, 4H), 4.19 (t, J=6.8 Hz, 1H), 3.23 (t, J=6.0 Hz, 2H), 4.17 (s, br, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 173.91, 155.10, 150.60, 149.53, 143.85, 141.42, 128.27, 128.20, 127.91, 127.22, 127.18, 125.12, 124.84, 120.17, 66.81, 62.60, 56.40, 47.25, 34.76, 25.37; MS (ESI +ve): calc (M+H) + : 571.11, found: 571.00; R f =0.5 (EtOAc). 
     Synthesis of Compound 59 
     
       
         
         
             
             
         
       
     
     Compound 57: Compound 57 is prepared from compound 56 (1 mmol) by reaction with 1.5 eq Fmoc-OSu in pyridine at r.t. for 18 h. Aqueous workup then column chromatography affords pure compound 57. 
     Compound 59: Compound 59 is prepared from compound 57 by a procedure analogous to that described for compound 55. 
     Synthesis of Compound 63 
     
       
         
         
             
             
         
       
     
     Compound 61: Compound 61 is prepared from compound 60 by a procedure analogous to that described for compound 57. 
     Compound 63: Compound 63 is prepared from compound 61 by a procedure analogous to that described for compound 55. 
     Synthesis of Compound 69 
     
       
         
         
             
             
         
       
     
     Compound 65: Ethyl isobutyrate, (11.6 g, 100 mmol) was added over 0.5 h to a cooled (−78° C.) solution of LDA in THF (53 mL, 2M). The orange mixture was then warmed to 0° C. briefly before re-cooling to −78° C. 1,2-dibromoethane (20 g, 106 mmol) was then added over 0.5 h to the solution which was warmed gradually to r.t. and stirred overnight. The resulting mixture was partitioned by addition of water and EA. Upon extraction into EA and washing with brine, the organic layer was separated then dried (MgSO 4 ), filtered and concentrated in vacuo. Column chromatography gave the pure product as a colorless oil (6.0 g, 25%).  1 H NMR (399 MHz, CDCl 3 ) 4.15 (q, J=7.1 Hz, 2H), 3.35 (t, J=8.4 Hz, 2H), 2.16 (t, J=8.4 Hz, 2H), 1.27 (t, J=7.1 Hz, 3H), 1.22 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 176.78, 60.83, 43.83, 42.89, 28.45, 25.20, 14.32; R f =0.5 (5% EtOAc/hexane). 
     Compound 66: A mixture of compound 65 (3.4 g, 15.24 mmol) and morpholine (13.28 g, 152 mmol) in THF (20 ml) was heated at 50° C. in a glass pressure bottle over 72 h. A white precipitate was observed to form. The mixture was separated between water and EA. The EA extracts were dried (MgSO 4 ), filtered and concentrated. Column chromatography gave the product as a colorless liquid (3.5 g, 100%).  1 H NMR (399 MHz, CDCl 3 ) 4.11 (q, J=7.1 Hz, 2H), 3.67 (t, J=4.7 Hz, 4H), 2.41 (t, br, J=4.0 Hz, 4H), 2.30-2.26 (m, 2H), 1.74-1.70 (m, 2H), 1.23 (t, J=7.1 Hz, 3H), 1.17 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 177.69, 67.11, 55.15, 54.02, 41.10, 37.04, 25.49, 14.35; MS (ESI +ve): calc (M+H) + : 230.18, found: 230.33; R f =0.5 (5% EtOAc/hexane). 
     Compound 67: Compound 66 (120 mg, 0.523 mmol) and sodium hydroxide (120 mg, 3.00 mmol) were stirred together in 1:1 EtOH/H 2 O (10 mL). After 36 h, the pH of the solution was adjusted to ca. 2 by addition of c. HCl, then NEt 3  was added until the pH reached ca. 10. SiO 2  (2 g) was added and the solvents/water were evaporated. Column chromatography with dry loading (MeOH/DCM, with 2% NEt 3  in the DCM), gave the product as a partial (approx ⅓ mol eq) HNEt 3  salt. The adjusted yield was 103 mg, 84%.  1 H NMR (399 MHz, CDCl 3  plus 3 drops of DMSO-d6) δ 3.62 (t, J=4.7 Hz, 4H), 2.51-2.46 (br, 4H), 2.40 (t, J=7.1 Hz, 2H), 1.62 (J=7.1 Hz, 2H), 1.08 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 179.91, 65.91, 54.29, 52.87, 45.44, 41.37, 35.09, 25.93, 8.56; MS (ESI −ve): calc (M−H) − : 200.13, found: 200.16; R f =0.5 (2% NEt 3 /10% MeOH/DCM). 
     Compound 69a: A suspension of compound 67 (11 g, 54.9 mmol) in DCM (200 mL) was treated with oxalyl chloride (9.42 ml, 110 mmol) and stirred at r.t. During the reaction a colorless homogeneous solution was formed. After 1 h at r.t. the solvent was removed under reduced pressure. The yellow residue was suspended in a mixture of DCM (200 mL) and Py (100 mL) then S-2-hydroxyethyl compound 54 (15.91 g, 60.4 mmol) (DB-7-5) was added all at once and the reaction was monitored by HPLC/MS. After 18 h, it was judged by MS and TLC that the reaction was virtually complete. The solvents were removed then the residue was redissolved in DCM/bicarbonate. The combined organics were dried (MgSO 4 ), filtered and reduced. Purification by column chromatography gave 12.5 g, 51% of compound 69a as a yellow oil which crystallized on standing.  1 H NMR (399 MHz, CDCl 3 ) δ 8.41 (d, J=8.5 Hz, 2H), 8.13 (d, J=8.5 Hz, 2H), 4.27 (t, J=6.3 Hz, 2H), 3.65 (t, J=4.6 Hz, 4H), 3.28 (t, J=6.3 Hz, 2H), 2.40 (s, br, 4H), 2.27 (t, J=7.7 Hz, 2H), 1.71 (t, J=7.7 Hz, 2H), 1.14 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 177.11, 150.71, 149.69, 128.34, 124.92, 66.96, 61.66, 54.95, 53.92, 41.25, 46.81, 35.11, 25.36; MS (ESI +ve): calc (M+H) + : 447.12, found: 446.98. 
     Compound 69b: A suspension of compound 67 (128 mg, 0.636 mmol) in DCM (12 mL) was treated dropwise with oxalyl chloride (545 μl, 6.36 mmol) and stirred at r.t. During the reaction a colorless homogeneous solution was formed which soon developed a colorless precipitate. HPLC/MS indicated good conversion to acid chloride (compound 68) as shown by conversion to the N-propyl amide. The solvent was removed under reduced pressure. The white residue was cooled on an ice bath and treated with a DCM (12 mL) solution of S-2-hydroxyethyl methanesulfonothioate (compound 54) (99 mg, 0.636 mmol), followed by dropwise addition of Hunig&#39;s base (0.34 g, 2.6 mmol, 4 eq) with stirring. After 1.5 h, the solution was washed with diluted NaHCO 3  (aq). The combined extracts were dried (MgSO 4 ), filtered and concentrated. Column chromatography gave the product as a colorless oil (84 mg, 39%).  1 H NMR (399 MHz, CDCl 3 ) δ 4.40 (t, J=6.2 Hz, 2H), 3.70 (t, J=4.6 Hz, 4H), 3.44 (t, J=6.2 Hz, 2H), 3.39 (s, 3H), 2.47-2.43 (br, 4H), 2.32 (t, J=7.7 Hz, 2H), 1.77 (t, J=7.7 Hz, 2H), 1.64-1.58 (br, 4H), 1.22 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 177.30, 67.08, 62.27, 55.03, 54.01, 51.07, 41.32, 36.99, 35.14, 25.44; MS (ESI +ve): calc (M+H) + : 340.13, found: 340.27; R f =0.2 (EtOAc). 
     Synthesis of Compound 73 
     
       
         
         
             
             
         
       
     
     Compound 70: Compound 70 was prepared by reaction of N-methyl piperazine with compound 65 by a procedure analogous to that described for compound 66.  1 H NMR (399 MHz, CDCl 3 ) δ 4.10 (q, J=7.1 Hz, 2H), 2.3-2.6 (br, 8H), 2.28 (t, J=8.0 Hz, 2H), 2.26 (s, 3H), 1.72 (t, J=8.0 Hz, 2H), 1.23 (t, J=7.1 Hz, 3H), 1.15 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 177.71, 60.44, 55.27, 54.64, 53.50, 46.18, 41.15, 37.36, 25.47, 14.34; MS (ESI +ve): calc (M+H) + : 243.20, found: 243.31. 
     Compound 71: Compound 71 was prepared by hydrolysis of compound 70 using a procedure analogous to that described for compound 67.  1 H NMR (399 MHz, CDCl 3 ) δ 12-10 (vbr, 1H), 3.2-2.2 (vbr, 8H), 2.44 (t, J=8.0 Hz, 2H), 2.36 (s, 3H), 1.71 (t, J=8.0 Hz, 2H), 1.17 (s, 6H),  13 C NMR (100 MHz, CDCl 3 ) δ 181.36, 55.18, 53.51, 52.18, 44.31, 41.57, 37.19, 26.28; MS (ESI +ve): calc (M+H) + : 215.17, found: 215.19. 
     Compound 83: Bromine (2.58 ml, 50.0 mmol) in DCM (50 mL) was added in a dropwise fashion over 0.5 h to a stirring mixture of sodium benzenesulfinate (16.4 g, 100 mmol) and 2-hydroxyethyl disulfide (6.11 ml, 49.9 mmol) in DCM (50 mL) at r.t. After 2 h at r.t. TLC showed good reaction (Rf=0.4 (1:1 EA/hexane), so the mixture was filtered to remove salt. The solvent was removed then column chromatography gave the product (17.4 g, 80%) as a colorless oil. 
     Compound 73: Compound 71 (1.342 g, 6.29 mmol), in DCM (20 mL) was treated with oxalyl chloride (1.079 ml, 12.58 mmol) and stirred at r.t. During the reaction a pale yellow precipitate was observed to form. After 0.5 h at r.t. the solvent was removed under reduced pressure. The pale yellow residue was suspended in a mixture of DCM (20 mL) and Py (20 mL) then compound 83 (2.060 g, 9.44 mmol) was added all at once. After 18 h, it was judged by TLC that the reaction was complete. The solvents were removed then the residue was redissolved in water and washed with ether. The aqueous layer was reduced to a brown solid. Recrystallization from boiling EtOH (10 mL) gave a slightly impure HCl salt which was free based (NEt 3  in DCM) then loaded directly onto silica gel. Column chromatography gave 0.95 g, 36% of pure compound 73.  1 H NMR (399 MHz, CDCl 3 ) δ 7.96 (d, J=8.0 Hz, 2H), 7.68 (t, J=7.2 Hz, 1H), 7.59 (t, J=7.7 Hz, 2H), 4.25 (t, J=6.3 Hz, 2H), 3.25 (t, J=6.3 Hz, 2H), 2.8-2.3 (vbr, 8H), 2.36 (s, 3H), 2.33 (t, J=7.7 Hz, 2H), 1.74 (t, J=7.7 Hz, 2H), 1.16 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 177.13, 144.68, 134.09, 129.59, 127.09, 61.99, 54.86, 54.35, 52.84, 45.95, 45.71, 41.26, 37.05, 34.66, 25.39; MS (ESI +ve): calc (M+H) + : 415.17, found: 415.09. 
     Synthesis of Compound 76 
     
       
         
         
             
             
         
       
     
     Compound 75: Compound 74 was converted to compound 75 by a procedure analogous to that described for compound 2.  1 H NMR (500 MHz, CDCl 3 ) δ 6.49-6.41 (br, d, J=6.3 Hz, 1H), 4.91 (dt, J=6.3, 2.7 Hz, 1H), 4.59 (ddd, J=31.4, 10.6, 3.0 Hz, 2H), 3.84 (s, 3H), 3.04 (s, 3H), 2.09 (s, 3H); 170.27, 169.05, 68.90, 53.33, 52.03, 37.63, 23.16; MS (ESI +ve): calc (M+H) + : 240.06, found: 240.24. 
     Compound 76: Compound 75 was converted to compound 76 by a procedure analogous to that described for compound 3.  1 H NMR (500 MHz, CDCl 3 ) δ 6.56-6.40 (br, d, J=6.2 Hz, 1H), 4.93 (q, J=5.9 Hz, 1H), 3.81 (s, 3H), δ 3.74 (dd, J=14.6, 4.8 Hz, 1H), 3.57 (dd, J=14.6, 5.6 Hz, 1H), 3.38 (s, 3H), 2.07 (s, 3H);  13 C NMR (126 MHz, CDCl 3 ) δ 170.44, 170.19, 53.23, 52.02, 50.73, 37.77, 23.12; MS (ESI +ve): calc (M+H) + : 256.03, found: 256.21. 
     Synthesis of Compound 78 
     
       
         
         
             
             
         
       
     
     A mixture of compound 77, (2.49 ml, 32.8 mmol), and sodium methanesulfonothioate (4.4 g, 32.8 mmol) was stirred in acetone (80 ml) over 6 h. The acetone was removed by rotary evaporation and the mixture was triturated with DCM. After filtration, DCM was removed by evaporation (5 g crude yield) and the mixture was subjected to purification by column chromatography. Yield of pure compound 78 was 2.8 g, 55%, colorless oil, Rf=0.3 (20% EA/hexane).  1 H NMR (500 MHz, CDCl 3 ) δ 5.30 (s, 2H), 3.48 (s, 3H), 3.38 (s, 3H);  13 C NMR (126 MHz, CDCl 3 ) δ 80.07, 57.40, 22.71. 
     Synthesis of Compound 80 
     
       
         
         
             
             
         
       
     
     An ice-cold solution of compound 79 (5 g, 65.7 mmol) and triethylamine (11 ml, 79 mmol) in DCM (150 mL) was treated with methanesulfonyl chloride (5.59 ml, 72.3 mmol) over 2 min. After 1 h the reaction was judged to be complete by TLC. Water was added and the organic extracts were washed sequentially with dilute HCl, saturated sodium bicarbonate, brine, then dried (MgSO 4 ), filtered and reduced. The product mesylate (9.4 g, 61 mmol, 96%) was taken up in acetone (200 ml), then the potassium salt of toluene thiosulfinic acid (61 mmol) was added and the solution was stirred at 50° C. over 18 h. A thick white precipitate was observed to form. The mixture was filtered, reduced to an oil then extracted into DCM/water. The organic extracts were dried (MgSO 4 ), filtered and reduced. Column chromatography gave pure compound 80 as a colorless oil which crystallized on standing Rf=0.3 (20% EA/hexane) 
     Synthesis of Compound 82 
     
       
         
         
             
             
         
       
     
     A mixture of 2-chloro-N,N-dimethylethanamine hydrochloride (10 g, 69.4 mmol), sodium iodide (1.041 g, 6.94 mmol) and potassium 4-methylbenzenesulfonothioate (18.86 g, 83 mmol) was stirred in MeOH (50 ml) and heated at 60° C. over 72 h. Aqueous workup then column chromatography gave pure compound 82 (8.5 g, 47%) as a colorless oil.  1 H NMR (399 MHz, CDCl 3 ) δ 7.81 (d, J=8.4 Hz, 2H), 7.33 (dd, J=8.4, 0.6 Hz, 2H), 3.09 (t, J=6.8 Hz, 2H), 2.53 (t, J=6.8 Hz, 2H), 2.44 (s, 3H), 2.17 (s, 6H);  13 C NMR (100 MHz, CDCl 3 ) δ 144.76, 142.03, 129.92, 127.18, 57.51, 45.14, 34.29, 21.77; MS (ESI +ve): calc (M+H) + : 260.07, found: 260.16. 
     Synthesis of Compound 85 
     
       
         
         
             
             
         
       
     
     Sodium methanesulfonate (11.5 g, 86 mmol) was placed in a dry 50 mL one neck flask. 30 mL of anhydrous DMF (Aldrich) was added using a dry glass syringe. 5 mL of 3-bromopropionitrile (compound 84) (8.2 g, 61.2 mmol) was added using a dry glass syringe. The reaction flask was closed under argon, sealed and stirred for 24 h at 50° C. The reaction was monitored using TLC (system: hexanes/ethyl acetate—5:5—v/v). The reaction mixture was diluted with 100 mL of ethyl acetate and washed 5 times with water (5×100 mL). The organic layers were dried over sodium sulfate, filtered and evaporated to dryness. The residue was dissolved in 5 mL of dichloromethane and purified by silica gel chromatography (CombiFlash) using a linear gradient of ethyl acetate in hexanes. Pure compound 85 (7.2 g, 52%) was obtained as a colorless oil.  1 H-NMR (CDCl 3 , 399 MHz). δ 3.43 (s, 3H), 3.41 (t, 2H, J=2.5 Hz), 2.93 (t, 2H, J=2.5 Hz);  13 C-NMR (CDCl 3 , 100 MHz) δ 117.7, 51.2, 31.7, 19.6. 
     Synthesis of Compound 87 
     
       
         
         
             
             
         
       
     
     Compound 87: Compound 86 (1.11 mL, 12.5 mmol) and sodium 4-nitrobenzenesulfinate (5.23 g, 25.0 mmol) were added in DCM (12.5 mL) to give a white suspension. Dibromine (0.64 ml, 12.5 mmol) was added to the stirred solution. The solution was stirred for 30 min at r.t. and filtrated to remove the salt, then the filtrate was evaporated under reduced pressure. The crude product was added to a silica gel column and was eluted with hexane-EtOAc to give pure compound 87 (4.80 g, 20.6 mmol, 82% yield).  1 H NMR (399 MHz, CDCl 3 ) δ 8.44-8.40 (m, 2H), 8.15-8.10 (m, 2H), 2.58 (s, 3H);  13 C NMR (100 MHz, CDCl 3 ) δ 149.00, 128.54, 124.87, 94.61, 18.55; R f =0.60 (1:1 EtOAc/hexane). 
     Synthesis of Compound 90 
     
       
         
         
             
             
         
       
     
     Compound 89: In a 1 L round-bottomed flask N-(2-mercaptoethyl)acetamide, 88 (11.92 g, 100 mmol) was dissolved in EtOAc (300 mL) to give a colorless solution. Sodium iodide (0.150 g, 1.000 mmol) and 30% hydrogen peroxide (3.40 g, 100 mmol) in H 2 O (11.3 mL) were added to the solution which was stirred for 45 min at r.t. Saturated Na 2 S 2 O 3  was added to the solution. The aqueous layer was extracted with EtOAc (3×300 mL). The combined organic layers were dried with Na 2 SO 4 , filtered and concentrated by rotary evaporation. The crude product was subjected to column chromatography and was eluted with a MeOH/DCM gradient to give pure compound 89 (4.94 g, 20.90 mmol, 41.8% yield), which was judged by TLC to be sufficiently pure for direct use in the next step of the reaction. R f =0.35 (9:1 DCM/Methanol) 
     Compound 90: In a 100 mL round-bottomed flask, compound 89 (2.364 g, 10.00 mmol) and sodium 4-nitrobenzenesulfinate (4.18 g, 20 mmol) were dissolved in CH 2 Cl 2  (20 mL) to give a white suspension. Dibromine (0.516 ml, 10.00 mmol) was added to the stirred solution. The solution was stirred for 30 min at r.t. and filtrated to remove the salt, then the filtrate was evaporated under reduced pressure. The crude product was added to a silica gel column and was eluted with hexane-EtOAc to give pure compound 90 (2.37 g, 7.79 mmol, 39% yield).  1 H NMR (399 MHz, CDCl 3 ) δ 8.45-8.40 (m, 2H), 8.17-8.12 (m, 2H), 3.60-3.54 (dt, 2H), 3.21-3.16 (t, 2H), 2.00 (s, 3H);  13 C NMR (100 MHz, CDCl 3 ) δ 130.35, 126.27, 66.97, 58.20, 53.67, 51.52, 36.22, 35.16, 33.67; R f =0.40 (EtOAc). 
     Synthesis of Compounds 93 and 94 
     
       
         
         
             
             
         
       
     
     Compound 92: In a 250 mL round-bottomed flask, sodium 4-chlorobenzenesulfonothioate (14.5 g, 63.0 mmol) was dissolved in MeOH. Cis-1,4-dichloro-2-butene, 91 (3.16 g, 30.0 mmol) and sodium iodide (450 mg, 3.0 mmol) was added to the stirred solution. The solution was stirred for 3 h at r.t., then heated up to 50° C. After stirring for 18 h at 50° C., solvents were removed under reduced pressure. The resulting crude material was diluted with DCM (300 mL) and washed with water (1×100 mL). The aqueous layer was extracted with DCM (1×100 mL). Combined organic layers were dried with Na 2 SO 4 , filtered and concentrated. The crude product was subjected to column chromatography and was eluted with an EtOAc/hexane gradient to give compound 92 (5.99 g, 12.8 mmol, 43% yield).  1 H NMR (399 MHz, CDCl 3 ) δ 7.87-7.84 (m, 4H), 7.56-7.51 (m, 4H), 5.56-5.50 (m, 2H), 3.72-3.68 (m, 4H);  13 C NMR (100 MHz, CDCl 3 ) 130.35, 126.27, 66.97, 58.20, 53.67, 51.52, 36.22, 35.16, 33.67; R f =0.35 (1:3 EtOAc/hexane). 
     Compound 93: In a 100 mL round-bottomed flask compound 92 (3.09 g, 6.58 mmol) was dissolved in THF at 0° C. Triethylamine (459 μL, 3.29 mmol) and 2-methyl-2-propanethiol (742 μL, 6.58 mmol) were added to the stirred solution at 0° C. The solution was stirred for 20 min at 0° C., then triethylamine (230 μL, 1.65 mmol) was added to the stirred solution at 0° C. After stirring for 40 min at 0° C., the solvents were removed under reduced pressure. The resultant was subjected to column chromatography and was eluted with an EtOAc/hexane gradient to give compound 93 (1.77 g, 4.62 mmol, 70% yield).  1 H NMR (399 MHz, CDCl 3 ) δ 7.90-7.85 (m, 2H), 7.56-7.52 (m, 2H), 5.73-5.65 (m, 1H), 5.55-5.48 (m, 1H), 3.78-3.75 (d, 2H), 3.36-3.32 (d, 2H), 1.32 (s, 9H);  13 C NMR (100 MHz, CDCl 3 ) δ 143.54, 140.60, 130.90, 129.83, 128.66, 124.56, 48.29, 37.05, 33.35, 30.16; R f =0.60 (1:3 EtOAc/hexane). 
     Compound 94: Using a procedure analogous to that described for compound 93, and substituting 2-propanethiol for 2-methyl-2-propanethiol, pure compound 94 was obtained (1.13 g, 3.06 mmol, 72% yield).  1 H NMR (399 MHz, CDCl 3 ) δ 7.90-7.82 (m, 2H), 7.56-7.48 (m, 2H), 5.72-5.64 (m, 1H), 5.57-5.47 (m, 1H), 3.79-3.72 (d, 2H), 3.33-3.26 (d, 2H), 3.61-2.92 (m, 1H), 1.27 (d, 6H);  13 C NMR (100 MHz, CDCl 3 ) 143.28, 140.36, 131.37, 125.63, 124.55, 124.56, 41.32, 38.02, 35.81, 33.08, 22.54; R f =0.55 (1:3 EtOAc/hexane). 
     Synthesis of Compound 96 
     
       
         
         
             
             
         
       
     
     Compound 96: Compounds 9 (1.81 g, 5.50 mmol) and 54 (2.17 g, 8.25 mmol) were combined and dried by co-evaporation with anhydrous toluene (3×2 mL). The mixture was dissolved in dry 1,2-dichloroethane (16.5 mL). 4 Å molecular sieves were added to the solution and the mixture was stirred 30 min at r.t. Trimethylsilyltriflate (497 μL, 2.75 mmol) was added to the stirred solution. The solution was stirred for 9 h at r.t., then additional 54 (0.723 g, 2.75 mmol) was added to the stirred solution. After stirring 16 h at r.t., the solution was diluted with DCM (100 mL) and washed with sat. NaHCO 3  (1×100 mL). The aqueous layer was extracted with DCM (1×50 mL). The combined organic layers were dried with Na 2 SO 4 , filtered and concentrated by rotary evaporation. The resulting crude was subjected to column chromatography and was eluted with an EtOAc/hexane gradient to give compound 96 (2.21 g, 3.73 mmol, 68% yield).  1 H NMR (399 MHz, CDCl 3 ) δ 8.42-8.36 (d, 2H), 8.12-8.06 (d, 2H), 6.07-6.00 (d, 1H), 5.33-5.30 (d, 1H), 5.23-5.15 (dd, 1H), 4.69-4.63 (d, 1H), 4.15-4.04 (m, 3H), 4.04-3.87 (m, 2H), 3.84-3.73 (m, 1H), 3.22-3.12 (t, 2H), 2.12-1.92 (m, 12H);  13 C NMR (100 MHz, CDCl 3 ) 179.95, 170.74, 170.48, 150.77, 149.59, 128.47, 125.03, 101.40, 71.08, 70.02, 67.39, 66.92, 61.76, 51.12, 36.51, 23.72, 20.92; R f =0.25 (1:9 MeOH/DCM). 
     Synthesis of Compound 100 
     
       
         
         
             
             
         
       
     
     Compound 98: An ice-cold solution of compound 97 (4.87 g, 22.0 mmol) in dry pyridine (70 mL) was treated with phenoxyacetic anhydride (37.8 g, 132.0 mmol) and 4-dimethylaminopyridine (26.9 mg, 220 μmol). After stirring at r.t. for 12 h, the mixture was concentrated and co-evaporated with toluene in vacuo. The resultant was diluted by DCM (400 mL) and washed with sat. NaHCO 3  (1×400 mL). Upon extraction, the organic layer was separated, dried with Na 2 SO 4 , filtered and concentrated in vacuo. The resultant was subjected to column chromatography and was eluted with an EtOAc/hexane gradient to give pure compound 98 (16.7 g, 22.0 mmol, 100% yield).  1 H NMR (399 MHz, CDCl 3 ) δ 7.36-6.75 (m, 20H), 6.27-6.20 (d, 1H), 5.43-5.36 (m, 1H), 5.22-5.06 (m, 2H), 4.81-4.65 (m, 3H), 4.60-4.52 (m, 2H), 4.45-4.29 (m, 2H), 4.17-4.02 (m, 2H), 3.97-3.87 (m, 1H), 1.85 and 1.74 (d, 3H, rotamers);  13 C NMR (100 MHz, CDCl 3 ) δ 170.20, 168.78, 168.70, 168.36, 167.52, 157.56, 157.48, 157.40, 130.09, 129.70, 129.66, 129.60, 122.32, 122.01, 121.94, 114.61, 114.58, 114.58, 114.39, 91.93, 68.37, 68.25, 67.30, 64.54, 61.25, 46.43, 22.95; R f =0.35 (1:1 EtOAc/hexane). 
     Compound 99: Compound 98 (15.15 g, 20.0 mmol) was dissolved in ClCH 2 CH 2 Cl (40 mL) and trimethylsilyl trifluoromethanesulfonate (5.43 mL, 30.0 mmol) was added to the stirred solution at r.t. The solution was stirred for 24 h at 50° C., triethylamine (12.6 mL, 90.0 mmol) was added and the mixture was concentrated in vacuo. The resultant was purified by silica gel column chromatography (EtOAc (2.5% Et 3 N)-hexane) to give slightly impure compound 99 containing tiny amounts of phenoxyacetic acid. This material was used in the next step of the reaction scheme without further purification. 
     Compound 100: Compound 99 (3.63 g, 6 mmol) and S-2-hydroxyethyl-4-nitrobenzenesulfonothioate, 54 (2.76 g, 10.5 mmol) were dissolved in ClCH 2 CH 2 Cl (18 mL) to give a colorless solution. 4 Å Molecular sieves were added to the stirred solution at r.t. The solution was stirred at r.t. for 30 min then trimethylsilyl trifluoromethanesulfonate (0.543 ml, 3.00 mmol) was added. The mixture was stirred at r.t. for 24 h and TLC showed about 90% reaction completed. Additional 54 (237.0 mg, 0.90 mmol) was added to the mixture and it was stirred for further 36 h to complete the reaction. The mixture was diluted with DCM (100 mL) and washed with sat NaHCO 3  (1×100 mL). The aqueous layer was back-extracted with CH 2 Cl 2  (1×50 mL). The combined organic layers were dried with Na 2 SO 4 , filtered, concentrated and dissolved in 120 mL of Ac 2 O-pyridine (1:9, v/v). The mixture was stirred for 12 h then concentrated under reduced pressure. The resultant was diluted with CH 2 Cl 2  (100 mL) and washed with sat NaHCO 3  (1×100 mL). The aqueous layer was back extracted with CH 2 Cl 2  (1×50 mL). The combined organic layers were dried with Na 2 SO 4 , filtered and concentrated. The resultant was purified by silica gel column chromatography (EtOAc-hexane gradient) to give pure compound 100 (2.56 g, 2.94 mmol, 49.0% yield).  1 H NMR (399 MHz, CDCl 3 ) δ 8.70-8.62 (d, 2H), 8.41-8.33 (d, 2H), 7.65-7.03 (m, 15H), 6.35-6.27 (d, 1H), 5.81-5.72 (m, 2H), 5.06-4.97 (m, 3H), 4.92-4.85 (m, 2H), 4.83-4.62 (m, 2H), 4.58-4.44 (m, 2H), 4.34-4.26 (m, 2H), 4.26-4.15 (m, 1H), 4.06-3.95 (m, 1H), 3.51-3.40 (m, 2H), 2.22 and 2.18 (d, 3H, rotamers);  13 C NMR (100 MHz, CDCl 3 ) 171.17, 169.13, 169.05, 168.92, 157.86, 157.80, 157.76, 150.82, 149.63, 130.01, 129.96, 128.47, 125.06, 122.27, 122.23, 114.93, 114.74, 100.86, 70.64, 70.54, 65.36, 64.90, 62.21, 51.30, 36.54, 23.74; R f =0.60 (1:1 EtOAc/hexane). 
     Synthesis of Compound 104 
     
       
         
         
             
             
         
       
     
     Compound 102: Using a procedure analogous to that described for compound 98, and substituting compound 101 for compound 97, pure compound 102 is obtained. 
     Compound 103 Using a procedure analogous to that described for compound 99, and substituting compound 102 for compound 98, pure compound 103 is obtained. 
     Compound 104 Using a procedure analogous to that described for compound 100, and substituting compound 103 for compound 99, pure compound 103 is obtained. 
     Synthesis of Compounds 109 and 111 
     
       
         
         
             
             
         
       
     
     Compound 106: A solution of compound 105 (100 mmol) in dry 1,2-dichloroethane (300 mL) is treated dropwise via syringe with TiCl 4  (110 mmol) over 2 min. After refluxing for 16 h, TLC shows complete reaction. The crude is diluted with DCM and washed with sat. NaHCO 3 . The combined organic layers are dried with Na 2 SO 4 , filtered, concentrated and used for next reaction without further purification. 
     Compound 107: Thiourea (150 mmol) and compound 106 (100 mmol) are dissolved in acetone (200 mL) under Ar. The reaction mixture is heated to 60° C. and stirred. After 2 h, a white solid precipitation is removed by filtration. The precipitate is recrystallized. The filtered crystals and Na 2 S 2 O 5  (140 mmol) are added to a stirred mixture of DCM (500 mL) and H 2 O (250 mL). The reaction mixture is heated to reflux under Ar. After 3 h, the reaction mixture is cooled to r.t. and the phases are separated. The aqueous layer is back extracted with DCM. The combined organic layers are dried over Na 2 SO 4 , filtered and concentrated to give compound 107. 
     Compound 108: 1-Bromo-2-chloroethane (100 mmol), triethylamine (200 mmol) and compound 107 (100 mmol) are dissolved in acetonitrile (200 mL) under Ar. The reaction mixture is heated to 60° C. and stirred. TLC shows completed reaction. The mixture is diluted with DCM (500 mL) and washed with NaHCO 3  (250 mL). The organic layer is dried over Na 2 SO 4 , filtered, concentrated and purified to give compound 108. 
     Compound 109: compound 112 (120 mmol), sodium iodide (10.0 mmol) and compound 108 (100 mmol) are dissolved in MeOH (200 mL) under Ar. The reaction mixture is heated to 60° C. and stirred. TLC shows completed reaction. The mixture is diluted with DCM (500 mL) and washed with NaHCO 3  (250 mL). The organic layer is dried over Na 2 SO 4 , filtered, concentrated and purified to give compound 109. 
     Compound 110: 1-Bromo-4-chloro-2,3-cis-butene (100 mmol), triethylamine (200 mmol) and compound 107 (100 mmol) are dissolved in acetonitrile (200 mL) under Ar. The reaction mixture is heated to 60° C. TLC shows reaction is completed. The mixture is diluted with DCM (500 mL) and washed with NaHCO 3  (250 mL). The organic layer is dried over Na 2 SO 4 , filtered, concentrated and purified to give compound 110. 
     Compound 111: compound 112 (120 mmol), sodium iodide (10.0 mmol) and compound 110 (100 mmol) are dissolved in MeOH (200 mL) under Ar. The reaction mixture is heated to 60° C. TLC shows completed reaction. The mixture is diluted with DCM (500 mL) and washed with NaHCO 3  (250 mL). The organic layer is dried over Na 2 SO 4 , filtered, concentrated and purified to give compound 111. 
     Synthesis of Compound 115 
     
       
         
         
             
             
         
       
     
     Compound 113: 2-Chlorosulfonylacetonitrile (113) is prepared following the procedure of Sammes (as described in patent GB 1252903). 
     Compound 114: Sodium sulfide nonahydrate (65 mmol) is taken up in 100 mL water. The flask is kept in a water bath. Compound 113 (50 mmol) is added dropwise to the solution while stirring and gently warming the water bath. Sulfur is observed to appear and then disappear in the flask. Solvent is evaporated under vacuum and the residue is recrystallized from ethanol. Sodium cyanomethanesulfonothioate (compound 114) is thus obtained as a colorless crystalline solid. 
     Compound 115: Compound 114 (13.69 g, 86 mmol) is taken up, with stirring, in anhydrous DMF (30 mL) in a 100 mL round bottom flask. Then, 3-bromopropionitrile (5 mL, 8.2 g, 61.2 mmol) is added and the resulting mixture is stirred for 18 h at 50° C. with monitoring by TLC. On completion of reaction (complete consumption of 3-bromopropionitrile), the reaction mixture is diluted with EtOAc (100 mL) and the organic layer is washed with 5×20 mL H 2 O. The organic separated organic layer is then dried (MgSO 4 ), filtered and reduced to an oil by rotary evaporation. The crude oil is purified by column chromatography using an EtOAc/hexane gradient and the fractions containing pure material are collected and the solvent is removed by rotary evaporation then further drying in vacuo to furnish the pure compound 115 as a colorless oil. 
     Synthesis of Compound 118 
     
       
         
         
             
             
         
       
     
     Compound 116: Compound 51 (2.9 g, 8.91 mmol) in DCM (50 mL) was treated dropwise with oxalyl dichloride (0.53 ml, 6.15 mmol) then DMF (10 μL) and stirred at r.t. During the reaction a colorless homogeneous solution was formed. After 2 h at r.t. the solvent was removed under reduced pressure. The white residue was redissolved in DCM (20 mL) then added dropwise to a stirring pyridine (10 mL) solution of ethane-1,2-dithiol (8.40 g, 89 mmol), with monitoring by UPLC/MS. After 18 h, it was judged that the reaction was complete. The solvents were removed and the remaining ethanethiol was removed by trap-to-trap distillation then the residue was subjected to purification by column chromatography with DCM to provide the pure compound 116 as a colorless solid. Yield was 1.82 g, 51%. MS (ESI+): calc (M+Na) + : 424.10, found: 424.06.  1 H NMR (500 MHz, CDCl 3 ) δ 7.79 (d, J=7.5 Hz, 2H), 7.63 (d, J=5.7 Hz, 2H), 7.43 (t, J=7.5 Hz, 2H), 7.34 (t, J=7.4 Hz, 2H), 5.40-5.15 (br, 1H), 4.60-4.35 (br, 2H), 4.31-4.19 (m, 1H), 3.15-3.04 (br, 2H), 2.95-2.80 (m, 1H), 2.75-2.60 (br, 2H), 1.72-1.43 (m, 6H);  13 C NMR (126 MHz, CDCl 3 ) 203.14, 154.75, 143.92, 141.49, 127.84, 127.19, 125.17, 120.13, 66.81, 62.69, 47.41, 33.01, 25.78, 24.60. 
     Compound 117: To a stirred solution of compound 116 (1.7 g, 4.23 mmol) in EtOAc (12 mL) was added sodium iodide (6.35 mg, 0.042 mmol) and hydrogen peroxide (0.144 g, 4.23 mmol) (0.45 mL of a 30% aqueous solution) and the mixture was stirred at r.t. for 15 min. TLC showed complete consumption of the starting material. Aqueous sodium thiosulfate was added until the solution became colorless. The solution was washed with water, dried (MgSO 4 ), then column chromatography (EtOAc/hexane), gave pure compound 117 as a colorless solid foam (1.45 g, 86%). MS (ESI+): calc (M+H) + : 802.07, found: 802.08.  1 H NMR (500 MHz, CDCl 3 ) δ 7.78 (d, J=7.6 Hz, 4H), 7.63 (d, J=5.8 Hz, 4H), 7.42 (t, J=7.5 Hz, 4H), 7.33 (t, J=7.5 Hz, 4H), 5.42-5.25 (br, 2H), 4.55-4.35 (br, 4H), 4.30-4.18 (br, 2H), 3.25-3.10 (br, 4H), 2.95-2.70 (br, 4H), 1.65-1.45 (br, 12H);  13 C NMR (126 MHz, CDCl 3 ) δ 203.19, 154.71, 143.90, 141.45, 127.80, 127.17, 125.16, 120.10, 66.76, 62.65, 47.37, 37.90, 28.56, 25.73. 
     Compound 118: Dibromine (0.091 ml, 1.77 mmol) was added in a dropwise fashion over 2 min to a stirring mixture of sodium 4-nitrobenzenesulfinate (0.742 g, 3.55 mmol) and compound 117 (1.42 g, 1.773 mmol) in DCM (10 mL) at r.t. After 30 min stirring at r.t., the mixture was filtered and the filtrate was reduced to an orange solid foam in vacuo. Column chromatography (DCM/hexane) gave pure compound 118 (1.26 g, 60%) as a pale yellow solid foam. MS (ESI+): calc (M+H) + : 587.71, found: 802.08.  1 H NMR (500 MHz, CDCl 3 ) δ 8.36 (d, J=8.2 Hz, 2H), 8.14 (d, J=7.3 Hz, 2H), 7.79 (d, J=7.0 Hz, 2H), 7.63 (s, 2H), 7.43 (t, J=6.7 Hz, 2H), 7.35 (d, J=6.8 Hz, 2H), 5.35-5.15 (br, 1H), 4.55-4.35 (br, 2H), 4.30-4.20 (br, 1H), 3.30-3.00 (br, 4H), 1.70-1.25 (br, 6H);  13 C NMR (126 MHz, CDCl 3 ) δ 202.71, 154.66, 150.60, 149.63, 143.78, 141.46, 128.40, 127.91, 127.21, 125.12, 124.81, 120.18, 66.86, 62.59, 47.35, 35.84, 28.42, 25.61. 
     Synthesis of Compound 120 
     
       
         
         
             
             
         
       
     
     Compound 120: Commercially available compound 119 (1 g, 7.04 mmol) and potassium 4-methylbenzenesulfonothioate (1.753 g, 7.75 mmol) were stirred in MeOH (10 mL) over 5 days at r.t. then at 40° C. over 24 h. TLC showed good conversion to product. The MeOH was evaporated and the residue was taken up in DCM (30 mL) and Boc 2 O (1.54 g, 7.04 mmol) was added. The mixture was treated with triethylamine (1.030 ml, 7.39 mmol) in a dropwise fashion over 10 min at r.t. until the solution became practically homogeneous. Washing with water (50 mL), drying (MgSO 4 ), filtration and evaporation gave the crude product which was further purified by column chromatography to give pure compound 120 (1.64 g, 65%) as a colorless solid. MS (ESI+): calc (M+Na) + : 380.10, found: 380.11.  1 H NMR (399 MHz, CDCl 3 ) δ 7.80 (d, J=8.2 Hz, 2H), 7.34 (dd, J=8.5, 0.8 Hz, 2H), 5.54 (ddt, J=24.7, 16.6, 7.9 Hz, 2H), 4.65 (s, 1H), 3.77-3.66 (m, 4H), 2.45 (s, 3H), 1.43 (s, 9H);  13 C NMR (100 MHz, CDCl 3 ) δ 155.83, 145.05, 142.06, 131.80, 130.01, 127.15, 124.55, 79.72, 37.29, 32.75, 28.51, 21.79. 
     Synthesis of Compound 122 
     
       
         
         
             
             
         
       
     
     Compound 122: A solution of commercially available compound 121 (1 g, 3.59 mmol) in water (1 mL) was treated all at once with a solution of potassium hexafluorophosphate (0.662 g, 3.59 mmol) in water (10 mL) and agitated by shaking at r.t. for 5 min. The resulting solid was collected by filtration, washed with 2×3 mL water and dried in vacuo over KOH to give pure compound 122 as a colorless solid (1.01 g, 82%).  1 H NMR (300 MHz, CD 3 CN) δ 3.67-3.60 (m, 2H), 3.49 (s, 3H), 3.53-3.45 (m, 2H), 3.10 (s, 9H);  13 C NMR (126 MHz, CD 3 CN) δ 65.98, 54.05 (q, J=4.1 Hz), 51.11, 28.99;  19 F NMR (376 MHz, CD 3 CN) δ −73.15 (d, J=706.5 Hz);  31 P NMR (162 MHz, CD 3 CN) δ −143.50 (hept, J=706.6 Hz). 
     Synthesis of Compound 125 
     
       
         
         
             
             
         
       
     
     Compound 124: A DMF (50 mL) solution of commercially available compound 123 (10 mmol) is treated successively with tert-butyl-2-aminoacetate (22 mmol), diisopropylethylamine (50 mmol) and HBTU (24 mmol). After 1 h stirring at r.t., water (100 mL) is added and the resulting solution is extracted with EtOAc (2×50 mL) and washed with 5% NaHCO 3  (2×25 mL) then dilute brine (2×25 mL), dried (MgSO 4 ), filtered and reduced by rotary evaporation. Purification by column chromatography provides the pure compound 124. 
     Compound 125: Dibromine (0.091 ml, 1.77 mmol) is added in a dropwise fashion over 2 min to a stirring mixture of sodium 4-nitrobenzenesulfinate (0.742 g, 3.55 mmol) and di-tert-butyl-2,2′-((4,4′-disulfanediyl-bis(butanoyl))bis(azanediyl))diacetate (824 mg, 1.77 mmol) in DCM (10 mL) at r.t. After 30 min stirring at r.t., the mixture is filtered and the filtrate is reduced to a solid foam in vacuo. Column chromatography gives the pure product as a solid foam. 
     Synthesis of Compound 129 
     
       
         
         
             
             
         
       
     
     Compound 127: A solution of ethane-1,2-dithiol (1.130 g, 12 mmol) in TFA (10 mL) was treated all at once with commercially available compound 126 (0.89 g, 9.99 mmol). The solution became warm. After 1 h stirring at r.t. the volatiles were removed and the residue was subjected to column chromatography to give pure compound 127 as a colorless oil (0.95 g, 48%). R f =0.6 (5% MeOH/DCM);  1 H NMR (500 MHz, CDCl 3 ) δ 6.09-5.93 (br, 1H), 4.41 (d, J=6.4 Hz, 2H), 2.87-2.83 (m, 2H), 2.79 (ddd, J=9.7, 7.3, 3.5 Hz, 2H), 2.04 (s, 3H), 1.71 (t, J=8.0 Hz, 1H);  13 C NMR (126 MHz, CDCl 3 ) δ 170.25, 40.89, 35.22, 24.91, 23.44. 
     Compound 129: A warm (35° C.) DMF (10 mL) solution of commercially available compound 128 (939 mg, 3.0 mmol) was treated in a dropwise fashion over 5 min with a DCM (5 mL) solution of compound 127 (250 mg, 1.513 mmol). After checking TLC, the solvents were removed by rotary evaporation under high vacuum then the residue was triturated with DCM (50 mL), filtered, and the filtrate was washed with 5×5% NaHCO 3 , then was dried (MgSO 4 ), filtered and reduced to a pale yellow solid. Column chromatography gave pure compound 129 as a pale yellow solid. MS (ESI+): calc (M+H) + : 320.02, found: 320.34;  1 H NMR (500 MHz, CDCl 3 ) δ 9.28 (dd, J=2.7, 0.7 Hz, 1H), 8.43 (dd, J=8.9, 2.6 Hz, 1H), 7.93 (dd, J=8.9, 0.7 Hz, 1H), 6.10-5.90 (br, 1H), 4.41 (d, J=6.5 Hz, 2H), 3.13 (dd, J=8.6, 6.3 Hz, 2H), 2.93 (dd, J=8.6, 6.4 Hz, 2H), 2.00 (s, 3H);  13 C NMR (126 MHz, CDCl 3 ) δ 170.18, 168.57, 145.22, 142.22, 131.81, 119.59, 40.65, 38.59, 30.07, 23.39. 
     Synthesis of Compound 134 
     
       
         
         
             
             
         
       
     
     Compound 130: Compound 130 is prepared by a previously described method (Heterocycles, Vol 54, p 139, 2001). 
     Compound 132: Using an analogous procedure described previously for the synthesis of compound 126 (Organic Syntheses, Coll. Vol. 6, p. 5 (1988)), compound 131 (Tetrahedron Letters, 48(39), 7038-7041; 2007) is converted to compound 132 by gently heating in the presence of an aqueous formaldehyde solution containing K 2 CO 3 . 
     Compound 133: Using a procedure analogous to that described for the synthesis of compound 127 and substituting compound 132 for compound 126 and compound 130 for ethane-1,2-dithiol, pure compound 133 is thus obtained. 
     Compound 134: Using a procedure analogous to that described for the synthesis of compound 129 and substituting compound 133 for compound 127, pure compound 134 is thus obtained. 
     Synthesis of Compound 140 
     
       
         
         
             
             
         
       
     
     Compound 136: An ice-cold solution of commercially available compound 135 (50 g, 342 mmol), and methanol (277 mL) was treated dropwise with sulfuric acid (3.36 g, 34.2 mmol) over 10 min then warmed to r.t. gradually overnight. Most of the solvent was removed by rotary evaporation, then saturated aqueous NaHCO 3  was carefully added. The pH of the solution was adjusted to 1.9 by addition of 1 M HCl then extracted into EtOAc (200 mL), washed with diluted brine (50 mL), dried (MgSO 4 ), filtered and reduced to 29 g of colorless oil. Column chromatography gave pure compound 136 as a colorless solid (12.5 g, 23%).  1 H NMR (500 MHz, CDCl 3 ) δ 12.5-10.5 (vbr, 1H), 3.69 (s, 3H), 2.63 (s, 2H), 1.32 (s, 6H);  13 C NMR (126 MHz, CDCl 3 ) δ 183.32, 171.76, 51.72, 43.88, 40.60, 25.34. 
     Synthesis of Compound 138: Compound 136 (10 mmol) in DCM (50 mL) is treated dropwise with oxalyl dichloride (10 mmol) then DMF (10 μL) and stirred at r.t. During the reaction a colorless homogeneous solution is formed. After 2 h at r.t. the solvent is removed under reduced pressure. The white residue is redissolved in DCM (20 mL) then added dropwise to a stirring pyridine (10 mL) solution of compound 137 (10 mmol), with monitoring by TLC. After 18 h, the solvents are removed and the residue is subjected to purification by column chromatography with DCM to provide the pure compound 137. 
     Compound 139: Compound 138 (5 mmol) as a solution in THF (20 mL) is added to an ice-cold solution of LiBH 4  (5 mmol) in THF (20 mL). Progress of the reaction at r.t. is judged by TLC. After completion of the reaction (complete consumption of starting material), water (100 mL) is added carefully to the ice-cold solution which is subsequently extracted with EtOAc (2×100 mL). The combined organic extracts are dried (MgSO 4 ), filtered and the solvents are removed by rotary evaporation. The residue is subjected to column chromatography the pure compound 139 is thus obtained. 
     Compound 140: Using a procedure analogous to that described for the synthesis of compound 80, and substituting compound 139 for compound 79, pure compound 140 is thus obtained. 
     Synthesis of Compound 143 
     
       
         
         
             
             
         
       
     
     Compound 139: Using a procedure analogous to that described for the synthesis of compound 139 and substituting compound 136 for compound 138, pure compound 141 is thus obtained. 
     Compound 142: A solution of compound 141 (8.39 mmol) in DCM/MeOH (12/3 mL) is treated dropwise at 0° C. with a 2 M solution of (diazomethyl)trimethylsilane (1.05 g, 9.23 mmol) in ether over 30 min. After quenching excess reagent with AcOH, washing with water (2×5 mL), drying (MgSO 4 ), filtration, removal of solvent by rotary evaporation, then column chromatography the pure compound 142 is obtained. 
     Compound 143: Using a procedure analogous to that described for the synthesis of compound 80, and substituting compound 142 for compound 79, pure compound 143 is thus obtained. 
     Synthesis of Compound 147 
     
       
         
         
             
             
         
       
     
     Compound 144: Using a procedure analogous to that described for the synthesis of compound 69a, and substituting compound 136 for compound 67, and compound 54 for NH 4 Cl plus iPr 2 NEt, pure compound 144 is thus obtained. 
     Compound 145: LiAlH 4  (100 mL of a 2 M solution in THF) is added in a dropwise fashion to an ice-cold THF (500 mL) solution of compound 144 (86 mmol). The mixture is warmed to r.t. then refluxed for 0.5 h. The residual LiAlH 4  is destroyed by careful addition of saturated Na 2 SO 4  to the ice-cold solution until a granular precipitate is formed. The mixture is filtered and reduced then redissolved in EtOAc, dried (MgSO4), filtered and reduced in vacuo to furnish compound 145 which is sufficiently pure for direct use in the next step of the reaction. 
     Compound 146: Using a procedure analogous to that described for the synthesis of compound 124, and substituting compound 145 for compound 123, and Leu-Fmoc-OH for tert-butyl 2-aminoacetate, pure compound 146 is thus obtained. 
     Compound 147: Using a procedure analogous to that described for the synthesis of compound 80, and substituting compound 146 for compound 79, and compound 114 for potassium 4-methylbenzenesulfonothioate, pure compound 147 is thus obtained. 
     Synthesis of Compound 150 
     
       
         
         
             
             
         
       
     
     Compound 148: Commercially available chloromethyl pivalate (10 mmol) is added in a dropwise fashion to a stirring solution of compound 130 (20 mmol) in 1,2-dichloroethane (100 mL) under Ar. The resulting solution is heated in order to force to completion (noted by disappearance of chloromethylpivalate material by TLC). Solvent is removed by rotary evaporation, then the residue is subjected to column chromatography to provide the pure compound 148. 
     Compound 149: Using a procedure analogous to that described for the synthesis of compound 89, and substituting compound 148 for compound 88, pure compound 149 is thus obtained. 
     Compound 150: Using a procedure analogous to that described for the synthesis of compound 87, and substituting compound 149 for compound 86, pure compound 150 is thus obtained. 
     Additional Sulfurization Reagents: 
                         
wherein S a  denotes any of the following structures:
 
                         
Synthesis of Phosphoramidites
 
     In some embodiments, the present invention provides phosphoramidites, and methods of making the same. In some embodiments, the provided phosphoramidites were used in the synthesis of oligonucleotides described in the present application. Exemplary phosphoramidites and their synthesis are described below. 
     Synthesis of Compound 203 
     
       
         
         
             
             
         
       
     
     Compound 202: N 4 -Bz-5′-O-DMTr-2′-O-MOE-5-methylcytidine (201) (2.00 g, 2.77 mmol) was treated with 2 M ammonia in 2-propanol (Aldrich, anhydrous, 45 mL) and Pyridine (22.5 mL) at 60° C. for 5 h then at r.t. overnight, with monitoring by TLC and UPLC/MS. Solvent was removed (3× azeotrope with toluene) then column chromatography (0-100/MeOH/DCM) gave the pure compound 202 (1.62 g, 95%) as a colorless solid which was homogeneous by TLC and HPLC. (ESI+): calc (M+H) + : 618.28, found: 618.53.  1 H NMR (399 MHz, CDCl 3 ) δ 7.82 (d, J = 1.3 Hz, 1H), 7.47-7.40 (m, 2H), 7.37-7.18 (m, 8H), 6.83 (dd, J=8.9, 1.7 Hz, 4H), 5.93 (d, J=1.1 Hz, 1H), 4.43 (td, J=8.3, 4.9 Hz, 1H), 4.24 (ddd, J=11.7, 5.1, 3.0 Hz, 1H), 4.07 (dt, J=8.6, 2.4 Hz, 1H), 4.03-3.98 (m, 1H), 3.90 (ddd, J=11.7, 6.3, 3.3 Hz, 1H), 3.79 (d, J=1.0 Hz, 6H), 3.63-3.52 (m, 3H), 3.44 (dd, J=11.0, 3.0 Hz, 1H), 3.37 (s, 3H), 3.32 (d, J=9.4 Hz, 1H), 1.80-1.64 (s, br, 1H), 1.13 (s, 3H). 
     Compound 203: Isobutyric anhydride (0.48 ml, 2.9 mmol) was added dropwise to a solution of compound 202 (1.61 g, 2.61 mmol) in DMF (13 ml) at r.t. The solution was allowed to stir at r.t. over 24 h then the solvent was removed in vacuo at 35° C. Extraction into ether/bicarbonate then drying (MgSO 4 ), filtration and removal of the solvent gave the crude product as a colorless solid foam. Column chromatography (0-4% MeOH/DCM) gave the pure product (1.75 g, 98%) as a colorless solid foam which was homogenous by TLC and HPLC. (ESI+): calc (M+H) + : 688.32, found: 688.56.  1 H NMR (399 MHz, CDCl 3 ) δ 8.00-7.75 (br, 1H), 7.45-7.39 (m, 2H), 7.34-7.20 (m, 8H), 6.84 (dd, J=9.0, 1.2 Hz, 4H), 5.97 (s, 1H), 4.43 (d, J=6.1 Hz, 1H), 4.20-4.04 (m, 3H), 3.79 (d, J=0.9 Hz, 6H), 3.64-3.58 (m, 1H), 3.64-3.52 (m, 2H), 3.47-3.40 (m, 2H), 3.38 (s, 3H), 1.70-1.55 (br, 1H), 1.39 (d, J=1.0 Hz, 3H), 1.18 (d, J=6.9 Hz, 6H). 
     Synthesis of Compound 205 
     
       
         
         
             
             
         
       
     
     Compound 205: (R)-1-phenyl-1-((S)-pyrrolidin-2-yl)ethanol (4) was dried by azeotropic distillation with toluene (3×3 mL). A solution dried compound 4 (0.725 g, 3.79 mmol) and 4-methylmorpholine (0.833 ml, 7.58 mmol) in toluene (5 mL) was added to an ice-cold solution of trichlorophosphine (0.331 ml, 3.79 mmol) in toluene (5 mL) which was then warmed to r.t. for 1 h then filtered under Ar and reduced to an oil which was used in the next step of the reaction without further purification. 
     Synthesis of Compound 207 
     
       
         
         
             
             
         
       
     
     Compound 207: Using a procedure analogous to that described for the synthesis of compound 205, and substituting compound 206 for compound 204, compound 207 was obtained as a crude brown oil and was used in the next step of the reaction without further purification. 
     Synthesis of Compound 208 
     
       
         
         
             
             
         
       
     
     Compound 208: Compound 203 (1.74 g, 2.53 mmol) was dried by co-evaporation with pyridine (3×5 mL) then toluene (5×5 mL). The resulting dried 203 was dissolved in THF (15 mL), then triethylamine (2.47 ml, 17.7 mmol) was added and the solution was cooled to −78° C. by means of CO 2 (s)/acetone cooling bath. A THF solution (15 mL) of the crude compound 205 (3.79 mmol) was added dropwise over 0.5 h then gradually warmed to r.t. After 1 h at r.t., TLC indicated complete conversion of compound 203 to product. The mixture was washed into a separation funnel with chloroform (100 mL) then was extracted with NaHCO 3  (saturated, aqueous, 50 mL then 2×25 mL). At each extraction, the aqueous extracts were washed with an additional 1×10 mL of chloroform. The combined chloroform extracts were dried (MgSO 4 ), filtered, and concentrated by rotary evaporation at 32° C. The crude solid thus obtained was redissolved in DCM containing a few drops of triethylamine then subjected to column chromatography with a hexane/EtOAc gradient containing a steady concentration of 2% triethylamine to give pure compound 208 as a white solid foam.  1 H NMR (399 MHz, CD 3 CN) δ 7.92-7.80 (s, 1H), 7.52-7.47 (m, 2H), 7.40-7.24 (m, 12H), 6.88 (dd, J=9.0, 1.1 Hz, 4H), 5.94 (d, J=3.9 Hz, 1H), 4.79 (dt, J=9.6, 5.6 Hz, 1H), 4.31-4.27 (m, 1H), 4.27-4.22 (m, 1H), 3.87 (t, J=4.7 Hz, 2H), 3.75 (d, J=2.0 Hz, 6H), 3.66 (td, J=5.9, 5.5, 2.2 Hz, 1H), 3.58-3.53 (m, 2H), 3.50 (dd, J=11.1, 2.4 Hz, 1H), 3.38 (dd, J=11.0, 3.3 Hz, 1H), 3.31 (s, 3H), 2.85 (dq, J=10.5, 7.4, 6.9 Hz, 2H), 1.73 (s, 3H), 1.52 (d, J=1.0 Hz, 3H), 1.47-1.32 (m, 2H), 1.17 (dd, J=6.9, 0.7 Hz, 6H), 1.05-0.90 (m, 2H);  31 P NMR (162 MHz, CD 3 CN) δ 155.35. 
     Synthesis of Compound 210 
     
       
         
         
             
             
         
       
     
     Compound 210: Using a procedure analogous to that described for compound 208 and substituting compound 209 for compound 203 and compound 207 for compound 205, pure compound 210 was obtained as a colorless solid foam.  1 H NMR (399 MHz, CD 3 CN) δ 8.31-8.27 (m, 2H), 7.86 (d, J=1.2 Hz, 1H), 7.62-7.55 (m, 1H), 7.55-7.45 (m, 5H), 7.44-7.24 (m, 12H), 6.94-6.90 (m, 4H), 5.96 (d, J=3.7 Hz, 1H), 4.86-4.76 (m, 1H), 4.29 (dd, J=5.0, 3.8 Hz, 1H), 4.23 (dt, J=5.8, 2.8 Hz, 1H), 3.92-3.80 (m, 2H), 3.78 (s, 6H), 3.74 (ddd, J=7.1, 5.3, 2.1 Hz, 1H), 3.55 (t, J=4.7 Hz, 2H), 3.51 (d, J=2.3 Hz, 1H), 3.41 (dd, J=11.1, 3.4 Hz, 1H), 3.30 (s, 3H), 3.26 (ddd, J=10.3, 6.1, 2.2 Hz, 1H), 2.86 (ddt, J=10.1, 8.2, 7.2 Hz, 1H), 1.72 (d, J=0.7 Hz, 3H), 1.62 (d, J=1.1 Hz, 3H), 1.56-1.41 (m, 2H), 1.09-0.87 (m, 2H);  31 P NMR (162 MHz, CD 3 CN) δ 155.22. 
     Synthesis of Compound 212 
     
       
         
         
             
             
         
       
     
     Compound 212: Using a procedure analogous to that described for compound 208 and substituting compound 211 for compound 203, pure compound 212 was obtained as a colorless solid foam.  1 H NMR (399 MHz, CDCl 3 ) δ 9.25-8.70 (br, 1H), 7.69 (d, J=1.3 Hz, 1H), 7.46-7.40 (m, 2H), 7.40-7.17 (m, 12H), 6.81 (dd, J=9.0, 2.5 Hz, 4H), 6.09 (d, J=4.3 Hz, 1H), 4.78 (dt, J=9.6, 5.2 Hz, 1H), 4.36-4.26 (m, 2H), 3.94-3.88 (m, 2H), 3.74 (dd, J=4.0, 0.9 Hz, 6H), 3.69 (td, J=6.4, 2.9 Hz, 1H), 3.64-3.56 (m, 3H), 3.44-3.37 (m, 2H), 3.36 (d, J=0.9 Hz, 3H), 3.00 (dtd, J=10.3, 7.9, 6.4 Hz, 1H), 1.72 (s, 3H), 1.54-1.44 (m, 1H), 1.38 (dd, J=6.8, 5.4 Hz, 1H), 1.34 (d, J=1.2 Hz, 3H), 1.23-1.13 (m, 1H), 0.97-0.87 (m, 1H);  31 P NMR (162 MHz, CDCl 3 ) δ 158.18. 
     Synthesis of Compound 213 
     
       
         
         
             
             
         
       
     
     Compound 213: Using a procedure analogous to that described for compound 210 and substituting compound 211 for compound 209, pure compound 213 was obtained as a colorless solid foam.  1 H NMR (399 MHz, CDCl 3 ) δ 9.55-9.10 (br, 1H), 8.09 (d, J=1.5 Hz, 1H), 7.80-7.71 (m, 2H), 7.69-7.47 (m, 12H), 7.20-7.08 (m, 4H), 6.32 (d, J=2.9 Hz, 1H), 5.20-5.11 (m, 1H), 4.66-4.59 (m, 1H), 4.49-4.39 (m, 1H), 4.33-4.24 (m, 1H), 4.16-4.02 (m, 8H), 4.00-3.93 (m, 1H), 3.91-3.84 (m, 2H), 3.73 (dd, J=11.0, 2.5 Hz, 1H), 3.65-3.52 (m, 4H), 3.27-3.16 (m, 1H), 2.15 (s, 3H), 1.88-1.76 (m, 1H), 1.76-1.65 (m, 1H), 1.63-1.49 (m, 4H), 1.29-1.18 (m, 1H);  31 P NMR (162 MHz, CDCl 3 ) δ 160.08. 
     Synthesis of Compound 215 
                         
(i) TMSCl, NEt3, 0° C.; (ii) 2,4,6-trimethylbenzene-1-sulfonyl chloride, DMAP; (iii) 1-methylpyrrolidine, 3-hydroxypropanenitrile, DBU, 0° C.; (iv) MeOH/H2O, 4 d, room temperature
 
     Compound 215: (i) Transient TMS Protection: A solution of N 2 -isobutyryl-5′-O-DMTr-2′-O-MOE-guanosine (compound 214) (15.04 g, 21.1 mmol) and triethylamine (11.8 ml, 85 mmol) was taken up in ACN (200 mL) then chlorotrimethylsilane (10.4 ml, 82 mmol) was added dropwise to the ice-cold solution which was stirred at r.t. for 1 h with monitoring by TLC. Filtration and removal of solvent, then extraction (DCM/NaHCO 3 500 mL/200 mL), drying (MgSO 4 ), filtration, and removal of the solvent gave the crude compound which was used in the next step of the reaction without further purification. The compound was homogeneous by TLC (R f =0.6, (5% MeOH/DCM)). (ii) Activation of O 6  position for Cyanoethyl protection: The residue was redissolved in DCM (500 mL) then triethylamine (13.1 ml, 94 mmol) was added, followed by 2,4,6-trimethylbenzene-1-sulfonyl chloride (6.15 g, 28.1 mmol) and DMAP (0.14 g, 1.146 mmol). The mixture was stirred at r.t. for 3 h with monitoring by TLC until full disappearance of the starting material and emergence of a new spot by TLC (R f =0.9, (5% MeOH/DCM)). (iii) Cyanoethyl protection: 1-methylpyrrolidine (22.43 ml, 211 mmol) was added slowly in a dropwise fashion to the ice-cold solution with further reaction at 0° C. over 1 h, monitoring by TLC (new spot, R f =0.2, streak, (5% MeOH/DCM)). 3-hydroxypropanenitrile (14.40 ml, 211.0 mmol) then DBU (6.32 mL, 42.1 mmol) were added in a dropwise fashion successively to the still ice-cold solution and stirring was continued for additional 90 min, monitoring by TLC (new spot, R f =0.6, (5% MeOH/DCM)). In order to force the reaction almost to completion, a further 1.6 mL of DBU was added dropwise with careful monitoring by TLC. The mixture was poured onto NaH 2 PO 4  (50 g in 400 mL water), the organics were separated, and washed with dilute NaH 2 PO 4  (10 g in 200 mL water), dried (MgSO 4 ), filtered and reduced by rotary evaporation. (iv) TMS Deprotection: The residue thus obtained in the previous step was redissolved in MeOH (250 mL) then water was added in a dropwise fashion being careful not to induce precipitation. The dropwise addition was continued over 4 days with monitoring by TLC. Most of the solvent was removed and the residue was extracted (DCM/NaHCO 3  (500/200 mL), filtered and reduced. The residue was subjected to column purification (0-5% MeOH/DCM) to give pure compound 215 as a white foam which was homogeneous by TLC and HPLC. (ESI+): calc (M+H) + : 767.34, found: 767.03.  1 H NMR (300 MHz, CD 3 CN) δ 8.63 (s, 1H), 8.07 (s, 1H), 7.44-7.36 (m, 2H), 7.33-7.18 (m, 7H), 6.84-6.72 (m, 4H), 6.02 (d, J=3.2 Hz, 1H), 4.74 (td, J=6.2, 0.9 Hz, 2H), 4.71-4.64 (m, 2H), 4.14-4.06 (m, 1H), 3.88-3.73 (m, 8H), 3.57-3.41 (m, 4H), 3.32-3.23 (m, 3H), 3.08 (t, J=6.2 Hz, 2H), 2.77 (dt, J=13.7, 6.9 Hz, 1H), 1.14 (dd, J=6.9, 1.2 Hz, 6H). 
     Synthesis of Compound 216 
     
       
         
         
             
             
         
       
     
     Compound 216: Using a procedure analogous to that described for compound 208 and substituting compound 215 for compound 203, pure compound 216 was obtained as a colorless solid foam.  1 H NMR (399 MHz, CD 3 CN) δ 8.41 (s, 1H), 8.12 (s, 1H), 7.50-7.44 (m, 2H), 7.40-7.19 (m, 12H), 6.80 (dd, J=8.9, 5.2 Hz, 4H), 6.04 (d, J=5.7 Hz, 1H), 4.99 (t, J=5.5 Hz, 1H), 4.94 (ddd, J=9.9, 5.2, 3.7 Hz, 1H), 4.75 (t, J=6.2 Hz, 2H), 4.29 (q, J=3.8 Hz, 1H), 3.89-3.82 (m, 1H), 3.76-3.68 (m, 7H), 3.64 (ddd, J=6.8, 5.5, 2.3 Hz, 1H), 3.50-3.46 (m, 2H), 3.42 (s, 2H), 3.38-3.28 (m, 1H), 3.21 (s, 3H), 3.09 (t, J=6.2 Hz, 2H), 2.84 (dq, J=10.2, 7.3 Hz, 1H), 2.70-2.58 (m, 1H), 1.76 (s, 3H), 1.46-1.27 (m, 2H), 1.12 (d, J=6.8 Hz, 3H), 1.07 (d, J=6.8 Hz, 3H), 1.04-0.89 (m, 2H);  31 P NMR (162 MHz, CD 3 CN) δ 154.40. 
     Synthesis of Compound 217 
     
       
         
         
             
             
         
       
     
     Compound 217: Using a procedure analogous to that described for compound 210 and substituting compound 215 for compound 209, pure compound 217 was obtained as a colorless solid foam.  1 H NMR (399 MHz, CD 3 CN) δ 8.56 (s, 1H), 8.13 (s, 1H), 7.44 (dd, J=8.2, 1.5 Hz, 2H), 7.39-7.19 (m, 12H), 6.81 (dd, J=10.4, 8.9 Hz, 4H), 6.06 (d, J=4.5 Hz, 1H), 4.96-4.86 (m, 2H), 4.75 (t, J=6.2 Hz, 2H), 4.30 (td, J=5.1, 4.7, 2.4 Hz, 1H), 3.83 (dt, J=11.2, 4.3 Hz, 1H), 3.79-3.70 (m, 7H), 3.67 (ddd, J=7.1, 5.2, 2.0 Hz, 1H), 3.52-3.45 (m, 3H), 3.41 (dd, J=10.8, 2.7 Hz, 1H), 3.34-3.24 (m, 1H), 3.21 (s, 3H), 3.08 (t, J=6.1 Hz, 2H), 2.89-2.72 (m, 2H), 1.68 (s, 3H), 1.54-1.38 (m, 2H), 1.14 (dd, J=6.8, 5.4 Hz, 6H), 1.09-1.00 (m, 1H), 0.95-0.83 (m, 1H);  31 P NMR (162 MHz, CD 3 CN) δ 154.93. 
     Synthesis of Compound 219 
     
       
         
         
             
             
         
       
     
     Compound 219: Using a procedure analogous to that described for compound 208 and substituting compound 218 for compound 203, pure compound 219 was obtained as a colorless solid foam.  1 H NMR (399 MHz, CDCl 3 ) δ 9.31 (s, 1H), 8.72 (s, 1H), 8.26 (s, 1H), 8.07-7.98 (m, 2H), 7.60-7.53 (m, 1H), 7.52-7.43 (m, 4H), 7.38-7.15 (m, 12H), 6.83-6.74 (m, 4H), 6.24 (d, J=5.2 Hz, 1H), 5.03-4.92 (m, 2H), 4.45 (q, J=3.8 Hz, 1H), 3.93 (dt, J=11.4, 4.2 Hz, 1H), 3.77 (ddd, J=7.5, 5.8, 3.2 Hz, 2H), 3.73 (s, 6H), 3.60-3.49 (m, 3H), 3.47-3.37 (m, 2H), 3.26 (s, 3H), 3.02-2.92 (m, 1H), 1.81 (s, 3H), 1.56-1.43 (m, 1H), 1.37 (dq, J=13.2, 6.5 Hz, 1H), 1.22-1.09 (m, 1H), 0.96 (ddt, J=13.1, 7.9, 6.8 Hz, 1H);  31 P NMR (162 MHz, CDCl 3 ) δ 157.73. 
     Synthesis of Compound 220 
     
       
         
         
             
             
         
       
     
     Compound 220: Using a procedure analogous to that described for compound 210 and substituting compound 218 for compound 209, pure compound 220 was obtained as a colorless solid foam.  1 H NMR (399 MHz, CDCl 3 ) δ 9.26 (s, 1H), 8.73 (s, 1H), 8.31 (s, 1H), 8.02 (d, J=7.6 Hz, 2H), 7.60-7.53 (m, 1H), 7.52-7.40 (m, 4H), 7.39-7.13 (m, 12H), 6.84-6.76 (m, 4H), 6.24 (d, J=4.5 Hz, 1H), 4.99 (dt, J=10.0, 5.0 Hz, 1H), 4.84 (t, J=4.7 Hz, 1H), 4.46 (q, J=4.0 Hz, 1H), 3.91 (dt, J=11.1, 4.2 Hz, 1H), 3.85-3.69 (m, 8H), 3.63-3.50 (m, 3H), 3.42 (dd, J=10.7, 4.0 Hz, 1H), 3.40-3.31 (m, 1H), 3.27 (s, 3H), 3.00-2.89 (m, 1H), 1.82 (s, 3H), 1.58-1.38 (m, 2H), 1.20-1.09 (m, 1H), 1.00 (ddt, J=12.3, 8.8, 5.9 Hz, 1H);  31 P NMR (162 MHz, CDCl 3 ) δ 158.98. 
     Synthesis of Compound 222 
     
       
         
         
             
             
         
       
     
     Compound 222: Using a procedure analogous to that described for compound 208 and substituting compound 221 for compound 203, pure compound 222 was obtained as a colorless solid foam.  1 H NMR (499 MHz, CDCl 3 ) δ 10.46 (s, br, 1H), 8.62 (d, J=7.5 Hz, 1H), 7.42 (d, J=7.1 Hz, 2H), 7.36-7.21 (m, 12H), 7.00 (d, J=7.5 Hz, 1H), 6.82 (dd, J=9.0, 7.1 Hz, 4H), 6.03 (s, 1H), 4.77 (td, J=8.7, 4.8 Hz, 1H), 4.34 (dt, J=9.4, 2.3 Hz, 1H), 3.97 (d, J=4.8 Hz, 1H), 3.78 (dd, J=6.7, 3.4 Hz, 1H), 3.74 (s, 3H), 3.71 (s, 6H), 3.68-3.58 (m, 2H), 3.48-3.38 (m, 1H), 3.01 (p, J=7.8 Hz, 1H), 2.27 (s, 3H), 1.72 (s, 3H), 1.49 (tt, J=12.3, 7.0 Hz, 1H), 1.40 (dq, J=13.2, 6.6 Hz, 1H), 1.18 (dq, J=13.9, 7.0 Hz, 1H), 1.02-0.90 (m, 1H);  31 P NMR (202 MHz, CDCl 3 ) δ 158.71. 
     Synthesis of Compound 223 
     
       
         
         
             
             
         
       
     
     Compound 223: Using a procedure analogous to that described for compound 210 and substituting compound 221 for compound 209, pure compound 223 was obtained as a colorless solid foam.  1 H NMR (500 MHz, CDCl 3 ) δ 9.76 (s, 1H), 8.70 (d, J=7.4 Hz, 1H), 7.48-7.41 (m, 2H), 7.41-7.18 (m, 12H), 7.06 (d, J=7.4 Hz, 1H), 6.87 (dd, J=8.9, 1.9 Hz, 4H), 6.00 (s, 1H), 4.80 (td, J=9.2, 4.7 Hz, 1H), 4.33 (d, J=9.5 Hz, 1H), 3.84 (d, J=4.7 Hz, 1H), 3.81 (s, 3H), 3.80 (s, 3H), 3.72 (td, J=6.6, 3.4 Hz, 1H), 3.70-3.62 (m, 4H), 3.53 (dd, J=11.3, 2.2 Hz, 1H), 3.34 (tdd, J=10.7, 7.8, 4.9 Hz, 1H), 2.96 (dq, J=10.3, 7.6 Hz, 1H), 2.25 (s, 3H), 1.83 (s, 3H), 1.56-1.48 (m, 1H), 1.43 (dq, J=13.1, 6.4 Hz, 1H), 1.24-1.16 (m, 1H), 1.00-0.91 (m, 1H).  31 P NMR (202 MHz, CDCl 3 ) δ 157.17. 
     Synthesis of Compound 225 
     
       
         
         
             
             
         
       
     
     Compound 225: Using a procedure analogous to that described for compound 208 and substituting compound 224 for compound 203, pure compound 225 was obtained as a colorless solid foam.  1 H NMR (500 MHz, CDCl 3 ) δ 9.76 (s, br, 1H), 8.70 (d, J=7.4 Hz, 1H), 7.48-7.41 (m, 2H), 7.41-7.18 (m, 12H), 7.06 (d, J=7.4 Hz, 1H), 6.87 (dd, J=8.9, 1.9 Hz, 4H), 6.00 (s, 1H), 4.80 (td, J=9.2, 4.7 Hz, 1H), 4.33 (d, J=9.5 Hz, 1H), 3.84 (d, J=4.7 Hz, 1H), 3.81 (s, 3H), 3.80 (s, 3H), 3.72 (td, J=6.6, 3.4 Hz, 1H), 3.69-3.62 (m, 4H), 3.53 (dd, J=11.3, 2.2 Hz, 1H), 3.34 (tdd, J=10.7, 7.8, 4.9 Hz, 1H), 2.96 (dq, J=10.3, 7.6 Hz, 1H), 2.25 (s, 3H), 1.83 (s, 3H), 1.56-1.48 (m, 1H), 1.43 (dq, J=13.1, 6.4 Hz, 1H), 1.24-1.16 (m, 1H), 1.00-0.91 (m, 1H);  31 P NMR (202 MHz, CDCl 3 ) δ 158.97. 
     Synthesis of Compound 226 
     
       
         
         
             
             
         
       
     
     Compound 226: Using a procedure analogous to that described for compound 210 and substituting compound 224 for compound 209, pure compound 226 was obtained as a colorless solid foam.  1 H NMR (499 MHz, CDCl 3 ) δ 9.8-9.0 (br, 1H), 8.09 (d, J=8.2 Hz, 1H), 7.40 (d, J=7.4 Hz, 2H), 7.36-7.19 (m, 12H), 6.80 (dd, J=11.4, 8.8 Hz, 4H), 6.02 (d, J=2.2 Hz, 1H), 5.22 (d, J=8.1 Hz, 1H), 4.82 (td, J=8.1, 4.9 Hz, 1H), 4.27 (d, J=7.4 Hz, 1H), 3.96 (dd, J=5.0, 2.2 Hz, 1H), 3.81-3.76 (m, 1H), 3.74 (s, 3H), 3.70 (s, 3H), 3.62 (s, 3H), 3.61-3.54 (m, 2H), 3.50-3.41 (m, 1H), 3.04 (dtd, J=10.2, 8.0, 6.3 Hz, 1H), 1.75 (s, 3H), 1.56-1.47 (m, 1H), 1.44-1.35 (m, 1H), 1.26-1.18 (m, 1H), 0.95 (dq, J=12.3, 7.5 Hz, 1H);  31 P NMR (202 MHz, CDCl 3 ) δ 158.96. 
     Synthesis of Compound 228 
     
       
         
         
             
             
         
       
     
     Compound 228: Using a procedure analogous to that described for compound 208 and substituting compound 227 for compound 203, pure compound 228 was obtained as a colorless solid foam.  31 P NMR (202 MHz, CDCl 3 ) δ 158.16. 
     Synthesis of Compound 229 
     
       
         
         
             
             
         
       
     
     Compound 229: Using a procedure analogous to that described for compound 210 and substituting compound 227 for compound 209, pure compound 229 was obtained as a colorless solid foam.  31 P NMR (202 MHz, CDCl 3 ) δ 158.84. 
     Synthesis of Compound 231 
     
       
         
         
             
             
         
       
     
     Compound 231: Using a procedure analogous to that described for compound 208 and substituting compound 230 for compound 203, pure compound 231 was obtained as a colorless solid foam.  1 H NMR (499 MHz, CDCl 3 ) δ 9.6-9.3 (s, 1H), 8.73 (s, 1H), 8.32 (s, 1H), 7.50-7.41 (m, 2H), 7.39-7.16 (m, 14H), 7.10-7.01 (m, 3H), 6.82 (d, J=8.8 Hz, 4H), 6.21 (d, J=4.1 Hz, 1H), 4.96 (dt, J=10.1, 5.1 Hz, 1H), 4.86 (s, 2H), 4.54 (t, J=4.5 Hz, 1H), 4.43 (q, J=3.9 Hz, 1H), 3.81-3.75 (m, 7H), 3.62 (dd, J=10.8, 3.3 Hz, 1H), 3.54 (s, 3H), 3.46-3.42 (m, 1H), 3.41-3.33 (m, 1H), 3.01-2.93 (m, 1H), 1.82 (s, 3H), 1.56-1.47 (m, 1H), 1.44 (dq, J=13.2, 6.5 Hz, 1H), 1.22-1.11 (m, 1H), 1.03-0.94 (m, 1H);  31 P NMR (202 MHz, CDCl 3 ) δ 158.11. 
     Synthesis of Compound 232 
     
       
         
         
             
             
         
       
     
     Compound 232: Using a procedure analogous to that described for compound 210 and substituting compound 230 for compound 209, pure compound 232 was obtained as a colorless solid foam.  1 H NMR (499 MHz, CDCl 3 ) δ 9.8-9.2 (br, 1H), 8.71 (s, 1H), 8.23 (s, 1H), 7.49-7.43 (m, 2H), 7.40-7.15 (m, 14H), 7.09-7.02 (m, 3H), 6.79 (d, J=8.9 Hz, 4H), 6.20 (d, J=5.6 Hz, 1H), 4.93 (ddd, J=9.3, 5.0, 3.8 Hz, 1H), 4.86 (s, 2H), 4.73 (t, J=5.3 Hz, 1H), 4.43 (q, J=3.9 Hz, 1H), 3.79-3.75 (m, 1H), 3.74 (s, 3H), 3.74 (s, 3H), 3.57 (dt, J=6.8, 3.8 Hz, 1H), 3.52 (s, 3H), 3.46-3.36 (m, 2H), 2.99 (dtd, J=10.1, 7.9, 6.5 Hz, 1H), 1.80 (s, 3H), 1.54-1.45 (m, 1H), 1.39-1.31 (m, 1H), 1.23-1.13 (m, 1H), 0.99-0.90 (m, 1H);  31 P NMR (202 MHz, CDCl 3 ) δ 158.13. 
     Synthesis of Compound 234 
     
       
         
         
             
             
         
       
     
     Compound 234: Using a procedure analogous to that described for compound 208 and substituting compound 233 for compound 203, pure compound 234 was obtained as a colorless solid foam. 
     Synthesis of Compound 235 
     
       
         
         
             
             
         
       
     
     Compound 235: Using a procedure analogous to that described for compound 210 and substituting compound 233 for compound 209, pure compound 235 was obtained as a colorless solid foam.  31 P NMR (202 MHz, CDCl 3 ) δ 158.75. 
     Synthesis of Compound 237 
     
       
         
         
             
             
         
       
     
     Compound 237: Using a procedure analogous to that described for compound 208 and substituting compound 236 for compound 203, pure compound 237 was obtained as a colorless solid foam.  31 P NMR (202 MHz, Chloroform-d) δ 159.51 (d, J P-F =9.5 Hz). 
     Synthesis of Compound 238 
     
       
         
         
             
             
         
       
     
     Compound 238: Using a procedure analogous to that described for compound 210 and substituting compound 236 for compound 209, pure compound 238 was obtained as a colorless solid foam.  31 P NMR (202 MHz, Chloroform-d) δ 159.48. 
     Synthesis of Compound 240 
     
       
         
         
             
             
         
       
     
     Compound 240: Using a procedure analogous to that described for compound 208 and substituting compound 239 for compound 203, pure compound 240 was obtained as a colorless solid foam.  31 P NMR (202 MHz, CDCl 3 ) δ 160.20 (d, J P-F =11.0 Hz). 
     Synthesis of Compound 241 
     
       
         
         
             
             
         
       
     
     Compound 241: Using a procedure analogous to that described for compound 210 and substituting compound 239 for compound 209, pure compound 241 was obtained as a colorless solid foam.  31 P NMR (202 MHz, CDCl 3 ) δ 159.66 (d, J P-F =8.4 Hz). 
     Synthesis of Compound 243 
     
       
         
         
             
             
         
       
     
     Compound 243: Using a procedure analogous to that described for compound 208 and substituting compound 242 for compound 203, pure compound 243 was obtained as a colorless solid foam.  31 P NMR (202 MHz, CDCl 3 ) δ 160.20 (d, J=11.0 Hz). 
     Synthesis of Compound 244 
     
       
         
         
             
             
         
       
     
     Compound 244: Using a procedure analogous to that described for compound 210 and substituting compound 242 for compound 209, pure compound 244 was obtained as a colorless solid foam.  31 P NMR (202 MHz, CDCl 3 ) δ 156.52 (d, J P-F =8.5 Hz). 
     Synthesis of Compound 246 
     
       
         
         
             
             
         
       
     
     Compound 246: Using a procedure analogous to that described for compound 208 and substituting compound 245 for compound 203, pure compound 246 is obtained as a colorless solid foam. 
     Synthesis of Compound 247 
     
       
         
         
             
             
         
       
     
     Compound 247: Using a procedure analogous to that described for compound 210 and substituting compound 245 for compound 209, pure compound 247 is obtained as a colorless solid foam. 
     Synthesis of Compound 249 
     
       
         
         
             
             
         
       
     
     Compound 249: Using a procedure analogous to that described for compound 208 and substituting compound 248 for compound 203, pure compound 249 is obtained as a colorless solid foam. 
     Synthesis of Compound 250 
     
       
         
         
             
             
         
       
     
     Compound 250: Using a procedure analogous to that described for compound 210 and substituting compound 248 for compound 209, pure compound 250 is obtained as a colorless solid foam. 
     Synthesis of Compound 252 
     
       
         
         
             
             
         
       
     
     Compound 252: Using a procedure analogous to that described for compound 208 and substituting compound 251 for compound 203, pure compound 252 is obtained as a colorless solid foam. 
     Synthesis of Compound 253 
     
       
         
         
             
             
         
       
     
     Compound 253: Using a procedure analogous to that described for compound 210 and substituting compound 251 for compound 209, pure compound 253 is obtained as a colorless solid foam. 
     Synthesis of Compound 255 
     
       
         
         
             
             
         
       
     
     Compound 255: Using a procedure analogous to that described for compound 208 and substituting compound 254 for compound 203, pure compound 255 is obtained as a colorless solid foam. 
     Synthesis of Compound 256 
     
       
         
         
             
             
         
       
     
     Compound 256: Using a procedure analogous to that described for compound 210 and substituting compound 254 for compound 209, pure compound 256 is obtained as a colorless solid foam. 
     Synthesis of Compound 258 
     
       
         
         
             
             
         
       
     
     Compound 258: Using a procedure analogous to that described for compound 208 and substituting compound 257 for compound 203, pure compound 258 is obtained as a colorless solid foam. 
     Synthesis of Compound 259 
     
       
         
         
             
             
         
       
     
     Compound 259: Using a procedure analogous to that described for compound 210 and substituting compound 257 for compound 209, pure compound 259 is obtained as a colorless solid foam. 
     Examples 2-16 
     Summarized below in Table E-1 is the synthetic procedure for Examples 2-16 on the DNA/RNA Synthesizer ABI-394. 
     
       
         
           
               
             
               
                 TABLE E-1 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Examples 2-16. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time (sec) 
                 wait time (sec) 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 step 
                 reaction 
                 reagent 
                 1 μmol 
                 10 μmol 
                 1 μmol 
                 10 μmol 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 
                 3 + 90 + 
                 N.A. 
                 N.A. 
               
               
                   
                   
                   
                 10 
                 10 
                   
                   
               
               
                 2 
                 coupling 
                 0.15 M phosphoramidite in 
                 5 + 4 
                 10 + 6 
                 30 + 600 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2 M CMPT in ACN 
                   
                   
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 30 
                 60 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 
                 20 
                 30 
                 60 
                 60 
               
               
                   
                   
                 16% NMI in THF 
                   
                   
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3 M S-cyanoethyl- 
                 10 + 4 × 2 
                 15 + 4 × 4 
                 300 + 3 × 
                 300 + 3 × 
               
               
                   
                   
                 MethylThioSulfonate 
                   
                   
                 150 + 600 
                 150 + 600 
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Examples 2-9: The oligonucleotides containing stereodefined phosphorothioate diester internucleotidic linkages were synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-1 using a 10 μmol synthesis column and 6.5 μmol of succinyl linked dC on CPG. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry solid support was then treated with 5 mL of anhydrous 1 M solution of 1,8-diazabicycloundec-7-ene (DBU) in dry ACN-trimethylsilyl chloride—16:1 (v/v) for 10 min at r.t., during which time the solution was slowly pushed through the column by means of a plastic luer syringe fixed to the outlet of the column. The support was then washed with dry ACN and dried under vacuum. The dry CPG was placed in a plastic vial and then treated with 3 mL of 28% aqueous ammonia for a period of 18 h at r.t. The solvents were evaporated to dryness, the residue was re-suspended in 10% aqueous DMSO, and the solid support was filtered off. The crude product was purified by anion exchange preparative HPLC (Gradient of 0.25 to 1.75 M NaCl in 20 mM NaOH). The fractions having purity above 95% were pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product was lyophilized from water. 
     Examples 10-16: The oligonucleotides containing stereodefined phosphorothioate diester internucleotidic linkages were synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-1 using 1 μmol synthesis column and 3 μmol of succinyl linked dC on CPG. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry solid support was then treated with 5 mL of anhydrous 1 M solution of 1,5-diazabicyclo(4.3.0) non-5-ene (DBN) in dry ACN-trimethylsilyl chloride—16:1 (v/v) for 10 min at r.t. The DBN solution was slowly pushed through the column by means of a plastic luer syringe fixed to the outlet of the column. The support was then washed with dry ACN and dried under vacuum. The dry CPG was placed in a plastic vial and was treated with 2 mL of 28% aqueous ammonia for a period of 18 h at r.t. The solvents were evaporated to dryness, the residue was re-suspended in 10% aqueous DMSO, and the solid support was filtered off. 
     Purification and Desalting of Examples 2-16: 
     The crude product was purified by Waters 2525 BGM HPLC system, equipped with a 2487 dual wavelength detector and with FCO and Flex inject. An AP-1 glass column from Waters, 10×200 mm was filled with Source 15Q Support (GE Healthcare, Part no. 17-0947-01) and was used with flow rate of 4 mL/min. Column was heated during all the purifications using a TL600 mobile phase heater and TL150 Temperature controller (Timberline Instruments) set at 75° C. Buffer A: 20 mM NaOH and Buffer B: 20 mM NaOH, 2.5 M NaCl were used with step gradients starting from 30% B to 70% B. The fractions having purity above 95% were pooled, concentrated and desalted on the same HPLC system by reverse-phase column (XBridge Semi Prep, 250×10 mm, C 18 , 5 μm) with a gradient of water to 80% ACN and 4 mL/min flow rate. The final desalted product was concentrated in speedvac followed by lyophilization from water. 
     HPLC analysis of purified oligonucleotides: Quality of oligonucleotides was determined using DNA Pac 100 (10×250 mm) and using the following conditions: 
     Buffer A: 10 mM Tris HCl, 50% HCl, pH=8.0 
     Buffer B: A+0.8 M NaClO 4 , pH=8.0 
     Column temperature: 60° C. 
     Gradient method: 
                                                         Time   Flow   % A   % B   Curve                                                                    1   0.01   1.00   85.0   15.0               2   3.00   1.00   85.0   15.0   1           3   23.00   1.00   40.0   60.0   6           4   25.00   1.00   5.0   95.0   6           5   25.50   1.00   85.0   15.0   6           6   30.00   1.00   85.0   15.0   1                        
LCMS analysis method:
 
Eluent A: 15 mM TEA, 400 mM HFIP, Water
 
Eluent B: 50:50 Buffer A/Methanol
 
Column: UPLC@OST C 18  1.7 μm, 2.1×500 mm
 
Column temperature=50° C.
 
Gradient method:
 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time 
                 Flow 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                 0.2 
                 95 
                 5 
                   
               
               
                 10 
                 0.2 
                 35 
                 65 
                 6 
               
               
                 12 
                 0.2 
                 5 
                 95 
                 6 
               
               
                 12.5 
                 0.2 
                 95 
                 5 
                 6 
               
               
                 15 
                 0.2 
                 95 
                 5 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 2. Synthesis of Oligonucleotide 101 (All-(Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]) (SEQ ID NO: 106) 
     Oligonucleotide 101 was synthesized as described above. RT in IEX-HPLC: 14.70 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6310.4. 
     Example 3. Synthesis of Oligonucleotide 102 (All-(Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC]) (SEQ ID NO: 106) 
     Oligonucleotide 102 was synthesized as described above. RT in IEX-HPLC: 15.49 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6310.2. 
     Example 4. Synthesis of Oligonucleotide 103 ((Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) (5R-9S-5R)) 
     Oligonucleotide 103 was synthesized as described above. RT in IEX-HPLC: 15.10 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6310.3. 
     Example 5. Synthesis of Oligonucleotide 104 ((Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) (5S-9R-5S)) 
     Oligonucleotide 104 was synthesized as described above. RT in IEX-HPLC: 15.04 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6307.2. 
     Example 6. Synthesis of Oligonucleotide 105 ((Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) (1S-17R-1S)) 
     Oligonucleotide 105 was synthesized as described above. RT in IEX-HPLC: 14.75 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6310.2. 
     Example 7. Synthesis of Oligonucleotide 106 ((Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) (1R-17S-1R)) 
     Oligonucleotide 106 was synthesized as described above. RT in IEX-HPLC: 15.43 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6309.6. 
     Example 8. Synthesis of Oligonucleotide 107 ((Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) ((R/S) 9 R)) 
     Oligonucleotide 107 was synthesized as described above. RT in IEX-HPLC: 15.02 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6310.7. 
     Example 9. Synthesis of Oligonucleotide 108 ((Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) ((S/R) 9 S)) 
     Oligonucleotide 108 was synthesized as described above. RT in IEX-HPLC: 15.10 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6307.9. 
     Example 10. Synthesis of Oligonucleotide 109 ((Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp)d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) (3S-13R-3S)) 
     Oligonucleotide 109 was synthesized as described above. RT in IEX-HPLC: 14.91 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6309.5. 
     Example 11. Synthesis of Oligonucleotide 110 ((Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, RP)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) (3R-13S-3R)) 
     Oligonucleotide 110 was synthesized as described above. RT in IEX-HPLC: 15.24 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6309.3. 
     Example 12. Synthesis of Oligonucleotide 111 ((Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) ((18S/R 19 )) 
     Oligonucleotide 111 was synthesized as described above. RT in IEX-HPLC: 15.69 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6309.4. 
     Example 13. Synthesis of Oligonucleotide 113 ((Sp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) (18S/R 2 )) 
     Oligonucleotide 113 was synthesized as described above. RT in IEX-HPLC: 15.72 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6311.0. 
     Example 14. Synthesis of Oligonucleotide 114 ((Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) ((RRS) 6 -R)) 
     Oligonucleotide 114 was synthesized as described above. RT in IEX-HPLC: 14.14 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6313.7. 
     Example 15. Synthesis of Oligonucleotide 115 ((Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp)-d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) (S-(RRS) 6 )) 
     Oligonucleotide 115 was synthesized as described above. RT in IEX-HPLC: 14.30 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6313.7. 
     Example 16. Synthesis of Oligonucleotide 116 ((Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp)d[GsCsCsTsCsAsGsTsCsTsGsCsTsTsCsGsCsAsCsC] (SEQ ID NO: 106) (RS-(RRS) 5 -RR)) 
     Oligonucleotide 116 was synthesized as described above. RT in IEX-HPLC: 14.17 min. UPLC/ESI-MS: Calcd for C 191 H 246 N 67 O 102 P 19 S 19 : 6310.2; Found: 6312.4. 
     Results of Examples 2-16 are summarized in Table E-2, below: 
     
       
         
           
               
             
               
                 TABLE E-2 
               
             
            
               
                   
               
               
                 Summary of Examples 2-16. 
               
            
           
           
               
               
               
               
               
            
               
                 Oli- 
                   
                   
                 Descrip- 
                   
               
               
                 gonu- 
                 SEQ 
                   
                 tion of 
                 RT- 
               
               
                 cleo- 
                 ID 
                   
                 stereo- 
                 IEX 
               
               
                 tide 
                 NO: 
                 5′-sequence-3′ 
                 chemistry 
                 (min) 
               
               
                   
               
               
                 101 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 All-(Rp) 
                 14.70 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 102 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 All-(Sp) 
                 15.49 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 103 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 5R-9S-5R 
                 15.10 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 104 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 5S-9R-5S 
                 15.04 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 105 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 1S-17R-1S 
                 14.75 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 106 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 1R-17S-1R 
                 15.43 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 107 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 (R/S) 9 R 
                 15.02 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 108 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 (S/R) 9 S 
                 15.10 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 109 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 3S-13R-3S 
                 14.91 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 110 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 3R-13S-3R 
                 15.24 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 111 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 18S/R 19   
                 15.69 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 113 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 18S/R 2   
                 15.72 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 114 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 (RRS) 6 -R 
                 14.14 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 115 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 S-(RRS) 6   
                 14.30 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 116 
                 106 
                 d[GsCsCsTsCsAsGsTsCsT 
                 RS-(RRS) 5 - 
                 14.17 
               
               
                   
                   
                 sGsCsTsTsCsGsCsAsCsC] 
                 RR 
               
               
                   
               
            
           
         
       
     
     Example 17. Synthesis of Control Oligonucleotides 
     The control oligonucleotides (see Table E-3) were synthesized using the standard chemistry methods for automated solid-phase oligonucleotide synthesis (Beaucage, S. L. and Iyer, R. P.,  Tetrahedron,  1992, 48, 2223-2311). More specifically, stereorandom DNA was synthesized using standard DNA phosphoramidites (ChemGenes Co.), ethylthiotetrazole (ETT, Muang et al.,  Tetrahedron Lett.,  2004, 45, 6497-6499) as the activator (Glen Research) and N,N-dimethyl-N′-(3-thioxo-3H-1,2,4-dithiazol-5-yl)methanimidamide (DDTT, AM Chemicals) as the sulfurizing reagent (Guzaev, A.;  Tetrahedron Lett.,  2011, 52, 434-437). The phosphoramidite coupling time was 2 min and the sulfurization time was 10 min. The oligonucleotide was deprotected and purified using standard methods. DNA phosphodiester was synthesized using standard DNA phosphoramidites, ETT as the activator and iodine/pyridine/water as the oxidizing reagent. 2′-O-Methoxyethyl (MOE) DNA was synthesized using in-house prepared 2′-O-Methoxyethyl (MOE) phosphoramidites (Martin, P.;  Helv. Chim. Acta.  1995, 78, 486-504; Ross, B.; Song, Q.; 2004, US patent publication No. 20040082775), ETT as the activator and DDTT as the sulfurizing reagent. The times for coupling were 10 min and the times for sulfurization were 10 min. RNA was synthesized using standard RNA phosphoramidites (ChemGenes Co.), ETT as the activator and iodine/pyridine/water as the oxidizing reagent. The coupling times were 10 min. 
     All oligonucleotides were deprotected and purified using standard methods. 
     Purification of RNA (Oligonucleotide 117): 
     Waters 2525 BGM, 2487 Dual wavelength detector equipped with FCO and Flex injector 
     Buffer A: 20 mM Sodium Phosphate pH=8.5 
     Buffer B: 20 mM Sodium Phosphate, 1 M NaBr pH=8.5 
     Column: AP-1 glass column from Waters, 10×200 mm, filled with Super Q-5PW (20), TSK Gel (Anion Exchange) from TOSOH 
     Column Temperature: 70° C. (Timberline Instruments, TL600 mobile phase heater and TL150 Temperature controller) 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 100 
                 0 
                   
               
               
                 10 
                 4 
                 100 
                 0 
                 1 
               
               
                 25 
                 4 
                 80 
                 20 
                 6 
               
               
                 115 
                 4 
                 55 
                 45 
                 6 
               
               
                 125 
                 4 
                 0 
                 100 
                 6 
               
               
                 130 
                 4 
                 100 
                 0 
                 6 
               
               
                 140 
                 4 
                 100 
                 0 
                 1 
               
               
                   
               
            
           
         
       
     
     As shown in  FIG.  1   , the chirally controlled phosphorothioate diester 20-mer oligonucleotide (All-(Rp), Oligonucleotide 101,  FIG.  1   , A) has a different retention time than that of the phosphorothioate diester 20-mer standard stereorandom oligonucleotide (Oligonucleotide 118,  FIG.  1   , C) and has a sharper peak. One of skill in the art understands that during purification of the stereorandom oligonucleotide 108, it is likely that most of the all-(Rp) oligonucleotide (101, present in an approximately ½ 19  fraction of the mixture stereorandom oligonucleotide 108) would be lost. 
     Results of Example 17 are summarized in Table E-3, below. 
     
       
         
           
               
             
               
                 TABLE E-3 
               
             
            
               
                   
               
               
                 Summary of Example 17. 
               
            
           
           
               
               
               
               
               
            
               
                 Oligonu- 
                 SEQ ID 
                   
                   
                 RT 
               
               
                 cleotide 
                 NO: 
                 5′-sequence-3′ 
                 Description 
                 (min) 
               
               
                   
               
               
                 117 
                 114 
                 GGUGCGAAGCAGAC 
                 RNA 
                  5.10 
               
               
                   
                   
                 UGAGGC 
                   
                   
               
               
                   
               
               
                 118 
                 106 
                 d[GsCsCsTsCsAs 
                 stereo- 
                 15.04 
               
               
                   
                   
                 GsTsCsTsGsCsTs 
                 random 
                   
               
               
                   
                   
                 TsCsGsCsAsCsC] 
                   
                   
               
               
                   
               
               
                 119 
                 106 
                 d[GCCTCAGTC 
                 DNA 
                  6.90 
               
               
                   
                   
                 TGC TTC GCACC] 
                   
                   
               
               
                   
               
               
                 120 
                 106 
                 ( Gs5mCs5mCsTs5   
                 stereo- 
                 15.49 
               
               
                   
                   
                   mC s) MOE   
                 random 
                   
               
               
                   
                   
                 d[AsGsTs5mCsTs 
                   
                   
               
               
                   
                   
                 Gs5mCsTsTs5mCs] 
                   
                   
               
               
                   
                   
                 ( Gs5mCsAs5mCs5   
                   
                   
               
               
                   
                   
                 
                   mC) MOE   
                 
                   
                   
               
               
                   
               
               
                 121 
                 115 
                 d[GsAsTsGsCsCs 
                 scrambled 
                 15.09 
               
               
                   
                   
                 TsCsTsCsCsTsAs 
                   
                   
               
               
                   
                   
                 CsGsCsGsCsCsT] 
               
               
                   
               
            
           
         
       
     
     Procedures for Examples 18-21: The oligonucleotides containing stereodefined morpholinoethyl phosphorothioate triester internucleotidic linkages were synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-4 using 1 μmol synthesis column and 0.8 μmol of oxalyl linked dC on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product was purified by reverse phase preparative HPLC. The fractions having purity above 95% were pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product was lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-4 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Examples 18-21. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                 wait time 
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15 M phosphoramidite in ACN + 1.2 M CMPT 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 in ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 16% NMI in THF 
                 20 
                 60 
               
               
                 5 
                 sulfurization 
                 0.3 M S-Morpholinoethyl-ToluylThioSulfonate 
                 10 + 4 × 2 
                 300 + 3 × 
               
               
                   
                   
                   
                   
                 150 + 600 
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     General Purification Method for Examples 18-21: 
     Buffer A: 20 mM Phosphate pH=6.0 (adjusted with phosphoric acid) 
     Buffer B: ACN 
     Column: XBridge Prep C 18 , 5 μm, C 18 , 250×10 mm, Part #186003256 
     Buffer heater set temperature=50° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time 
                 Flow (ml/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 99 
                 1 
                   
               
               
                 5 
                 4 
                 99 
                 1 
                 1 
               
               
                 10 
                 4 
                 77 
                 23 
                 6 
               
               
                 60 
                 4 
                 70 
                 30 
                 6 
               
               
                 65 
                 4 
                 20 
                 80 
                 6 
               
               
                 70 
                 4 
                 20 
                 80 
                 6 
               
               
                 71 
                 4 
                 99 
                 1 
                 6 
               
               
                 80 
                 4 
                 99 
                 1 
                 1 
               
               
                   
               
            
           
         
       
     
     Analytical HPLC methods for oligonucleotides. 
     HPLC Method 1: 
     Buffer A: 20 mM Phosphate pH=6.0 (adjusted with phosphoric acid) 
     Buffer B: ACN 
     Column: XBridge C 18 , 3.5 μm, C 18 , 4.6×50 mm, Part #186003034 
     Buffer heater set temperature=35° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
                                             Time   Flow (ml/min)   % A   % B   Curve                                                    Initial       95   5           3   1   95   5   1       23   1   60   40   6       25   1   40   60   6       25.5   1   95   5   6       30   1   95   5   1                    
HPLC Method 2:
 
Buffer A: 50 mM TEAA, pH 7.8
 
Buffer B: ACN
 
Column: XBridge C 18 , 3.5 μm, C 18 , 4.6×50 mm, Part #186003034
 
Buffer heater set temperature=60° C.
 
Signal monitored at 254 and 280 nm
 
Gradient used:
 
                                             Time   Flow (ml/min)   % A   % B   Curve                                                    Initial       99   1           2   1   99   1   1       22   1   65   35   6       25   1   5   95   6       25.5   1   5   95   6       30   1   99   1   1                    
HPLC Method 3:
 
                                             Time   Flow (ml/min)   % A   % B   Curve                                                    Initial       85   15           2   1   85   15   1       20   1   60   40   6       22   1   5   95   6       25   1   5   95   6       25.5   1   85   15   6       30   1   85   15   1                    
HPLC Method 4:
 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time 
                 Flow (ml/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 85 
                 15 
                   
               
               
                 2 
                 1 
                 85 
                 15 
                 1 
               
               
                 20 
                 1 
                 40 
                 60 
                 6 
               
               
                 22 
                 1 
                 5 
                 95 
                 6 
               
               
                 25 
                 1 
                 5 
                 95 
                 6 
               
               
                 25.5 
                 1 
                 85 
                 15 
                 6 
               
               
                 30 
                 1 
                 85 
                 15 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 18. Synthesis of Oligonucleotide 122 (All-(Rp)-d[Gs1Cs1Cs1Ts1Cs1As1GsTs1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1Cs1As1Cs1C] (SEQ ID NO: 106) 
     
       
         
         
             
             
         
       
     
     Oligonucleotide 122 was synthesized as described above. RT in RP-HPLC: (HPLC method 1): 15.2 min. UPLC/ESI-MS: Calcd for C 305 H 455 N 86 O 121 P 19 S 19 : 8460.25; Found: 8462.0. 
     Example 19. Synthesis of Oligonucleotide 123 ((Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp)-d[Gs1Cs1Cs1Ts1Cs1As1Gs1Ts1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1Cs1As1Cs1C] (SEQ ID NO: 106) ((1S-17R-1S) 
     Oligonucleotide 123 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 16.2 min. UPLC/ESI-MS: Calcd for C 305 H 455 N 86 O 121 P 19 S 19 : 8460.3; Found: 8461.5. 
     Example 20. Synthesis of Oligonucleotide 124 (All-(Sp)-d[Gs1Cs1Cs1Ts1Cs1As1Gs1Ts1Cs1Ts1Gs1Cs1Ts1Ts1Cs1Gs1Cs1As1Cs1C] (SEQ ID NO: 106)) 
     Oligonucleotide 124 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 18.3 min. UPLC/ESI-MS: Calcd for C 305 H 455 N 86 O 121 P 19 S 19 : 8460.3; Found: 8461.8. 
     Example 21. Synthesis of Oligonucleotide 125 (All-(Rp)-d[5mCs1As1Ts1G] (SEQ ID NO: 106)) 
     Oligonucleotide 125 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 16.32 min. UPLC/ESI-MS: Calcd for C 58 H 85 N 18 O 22 P 3 S 3 : 1575.5; Found: 1575.2. 
     In Examples 22 and 42 the oligonucleotide containing stereodefined methoxyethyl phosphorothioate triester internucleotidic linkages was synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-5 using 1 μmol synthesis column and 0.8 μmol of oxalyl linked dC on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product is purified by reverse phase preparative HPLC (Gradient of 5 to 65% ACN in 20 mM sodium phosphate buffer, pH=6.0). The fractions having purity above 95% are pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product is lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-5 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Examples 22 and 42. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                 wait time 
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15 M phosphoramidite in ACN + 1.2 M CMPT 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 in ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 16% NMI in THF 
                 20 
                 60 
               
               
                 5 
                 sulfurization 
                 0.3 M S-MOE Toluylthiosulfonate 
                 10 + 4 × 2 
                 300 + 3 × 
               
               
                   
                   
                   
                   
                 150 + 600 
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 22. Synthesis of Oligonucleotide 126 (All-(Rp)-d[Cs2As2Gs2T] 
     
       
         
         
             
             
         
       
     
     Oligonucleotide 126 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 16.23 min. UPLC/ESI-MS: Calcd for C 48 H 68 N 15 O 22 P 3 S 3 : 1396.2; Found: 1395.2. 
     Example 23: The oligonucleotide containing stereodefined N-methylpiperazino bulky ester phosphorothioate triester internucleotidic linkages was synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-6 using 1 μmol synthesis column and 1.5 μmol of oxalyl linked dT on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product was purified by reverse phase preparative HPLC (Gradient of 5 to 65% ACN in 20 mM sodium phosphate buffer, pH=6.0). The fractions having purity above 95% were pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product was lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-6 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Example 23. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                 wait time  
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15 M phosphoramidite in ACN + 1.2 M CMPT in ACN 
                 5 + 4 
                 30 + 600 
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 16% NMI in THF 
                 20 
                 60 
               
               
                 5 
                 sulfurization 
                 0.3 M N-Methyl Piperazino ester PhenylThioSulfonate 
                 10 + 4 × 2 
                 300 + 3 × 
               
               
                   
                   
                   
                   
                 150 + 600 
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 23. Synthesis of Oligonucleotide 127 (All-(Rp)-d[Cs3As3Gs3T] 
     
       
         
         
             
             
         
       
     
     Oligonucleotide 127 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 20.24 min. UPLC/ESI-MS: Calcd for C 78 H 122 N 21 O 25 P 3 S 3 : 1943.1; Found: 1941.0. 
     Examples 24 and 43 The oligonucleotide containing stereodefined morpholino bulky ester phosphorothioate triester internucleotidic linkages was synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-7 using 1 μmol synthesis column and 1.5 μmol of oxalyl linked dT on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product is purified by reverse phase preparative HPLC (Gradient of 5 to 65% ACN in 20 mM sodium phosphate buffer, pH=6.0). The fractions having purity above 95% are pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product is lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-7 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Examples 24 and 43. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                 wait time 
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15 M phosphoramidite in ACN + 1.2 M CMPT in ACN 
                 5 + 4 
                 30 + 600 
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 16% NMI in THF 
                 20 
                 60 
               
               
                 5 
                 sulfurization 
                 0.3 M morpholino ester NitroPhenylThioSulfonate 
                 10 + 4 × 2 
                 300 + 3 × 
               
               
                   
                   
                   
                   
                 150 + 600 
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 24. Synthesis of Oligonucleotide 128 (All-(Sp)-d[Cs4As4Gs4T] 
     
       
         
         
             
             
         
       
     
     Oligonucleotide 128 was synthesized as described above. RT in RP-HPLC (HPLC method 3): 19.75 min. UPLC/ESI-MS: Calcd for C 75 H 113 N 18 O 28 P 3 S 3 : 1902.9; Found: 1904.0. 
     Example 25: The oligonucleotide containing stereodefined dimethylaminoethyl phosphorothioate triester internucleotidic linkages was synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-8 using 1 μmol synthesis column and 1.5 μmol of oxalyl linked dT on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product was purified by reverse phase preparative HPLC (Gradient of 5 to 65% ACN in 20 mM sodium phosphate buffer, pH=6.0). The fractions having purity above 95% were pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product was lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-8 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Example 25. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                 wait time 
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15 M phosphoramidite in ACN + 1.2 M CMPT 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 in ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 16% NMI in THF 
                 20 
                 60 
               
               
                 5 
                 sulfurization 
                 0.3 M dimethylaminoethyl-ToluylThioSulfonate 
                 10 + 4 × 2 
                 900 + 3 × 
               
               
                   
                   
                   
                   
                 600 + 900 
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 25. Synthesis of Oligonucleotide 129 (All-(Sp)-d[Cs5As5Gs5T] 
     
       
         
         
             
             
         
       
     
     Oligonucleotide 129 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 17.25 min. UPLC/ESI-MS: Calcd for C 51 H 77 N 18 O 19 P 3 S 3 : 1435.4; Found: 1435.0. 
     Example 26: The oligonucleotide containing stereodefined dimethylalanine ester phosphorothioate triester internucleotidic linkages was synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-9 using 1 μmol synthesis column and 1.5 μmol of oxalyl linked dT on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product is purified by reverse phase preparative HPLC (Gradient of 5 to 65% ACN in 20 mM sodium phosphate buffer, pH=6.0). The fractions having purity above 95% are pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product is lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-9 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Example 26. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                 wait time 
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15 M phosphoramidite in ACN + 1.2 M CMPT in ACN 
                 5 + 4 
                 30 + 600 
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 16% NMI in THF 
                 20 
                 60 
               
               
                 5 
                 sulfurization 
                 0.3 M dimethylalanine-Fmoc ester NitroPhenylThioSulfonate 
                 10 + 4 × 2 
                 900 + 3 × 
               
               
                   
                   
                   
                   
                 600 + 900 
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 26. Synthesis of Oligonucleotide 130 (All-(Sp)-d[Cs6As6Gs6T] 
     
       
         
         
             
             
         
       
     
     Oligonucleotide 130 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 16.45 min. UPLC/ESI-MS: Calcd for C 57 H 83 N 18 O 25 P 3 S 3 : 1609.5; Found: 1609.6. 
     Examples 27 and 28: The oligonucleotide containing stereodefined S-methyl phosphorothioate triester internucleotidic linkages was synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-10 using 1 μmol synthesis column and 0.8 μmol of oxalyl linked dC on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) with 50% DMSO for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product is purified by reverse phase preparative HPLC (Gradient of 5 to 65% ACN in 20 mM sodium phosphate buffer, pH=6.0). The fractions having purity above 95% are pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product is lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-10 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Examples 27 and 28. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                 wait time 
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 
                 20 
                 60 
               
               
                   
                   
                 16% NMI in THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-methyl 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                   
                 NitroPhenylThioSulfonate 
                   
                 600  
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 27. Synthesis of Oligonucleotide 131 (All-(Rp)-d[Gs7Cs7Cs7Ts7Cs7As7Gs7Ts7Cs7Ts7Gs7Cs7Ts7Ts7Cs7Gs7Cs7As7Cs7C] (SEQ ID NO: 106) 
     
       
         
         
             
             
         
       
     
     Oligonucleotide 131 was synthesized as described above. RT in RP-HPLC: 27.65 min. UPLC/ESI-MS: Calcd for C 210 H 284 N 67 O 102 P 19 S 19 : 6576.71; Found: 6575.6. 
     Example 28. Synthesis of Oligonucleotide 132 (All-(Sp)-d[Gs7Cs7Cs7Ts7Cs7As7Gs7Ts7Cs7Ts7Gs7Cs7Ts7Ts7Cs7Gs7Cs7As7Cs7C] (SEQ ID NO: 106) 
     
       
         
         
             
             
         
       
     
     Oligonucleotide 132 was synthesized as described above. RT in RP-HPLC: 32.65 min. UPLC/ESI-MS: Calcd for C 210 H 284 N 67 O 102 P 19 S 19 : 6576.71; Found: 6574.8. 
     Synthesis of Chirally Controlled Oligonucleotides Comprising Modified Nucleobases 
     As generally described above and herein, in some embodiments, the present invention provides chirally controlled oligonucleotides comprising oligonucleotides other than A, T, C and G. In some embodiments, such chirally controlled oligonucleotides comprise 5-methylcytosine (5mC). Non-limiting examples are presented in Examples 21 and below. 
     Examples 29-41 were synthesized using the automated synthesis on ABI-394 DNA/RNA synthesizer according to the synthetic cycle summarized on Table E-11, using 1 μmol synthesis column and 1.75 μmol of oxalyl linked dG on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). After completion of the automated oligonucleotide synthesis, the HCP support was washed with dry ACN and dried under vacuum. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product is purified by reverse phase preparative HPLC (According to the procedure described below). The fractions having purity above 95% are pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 30% ACN). The final desalted product is lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-11 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA 
               
               
                 Synthesizer ABI-394 Used for the Synthesis of Examples 29-41. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                   
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 
                 20 
                 60 
               
               
                   
                   
                 16% NMI in THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-Morpholinoethyl 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                   
                 ToluylThioSulfonate 
                   
                 600  
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     General Purification Method for Examples 29-41: 
     Buffer A: 20 mM Phosphate pH=6.0 (adjusted with phosphoric acid) 
     Buffer B: ACN 
     Column: XBridge Prep C 18 , 5 μm, C 18 , 250×10 mm, Part #186003256 
     Buffer heater set temperature=50° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time 
                 Flow (ml/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 99 
                 1 
                   
               
               
                 5 
                 4 
                 99 
                 1 
                 1 
               
               
                 10 
                 4 
                 77 
                 23 
                 6 
               
               
                 60 
                 4 
                 70 
                 30 
                 6 
               
               
                 65 
                 4 
                 20 
                 80 
                 6 
               
               
                 70 
                 4 
                 20 
                 80 
                 6 
               
               
                 71 
                 4 
                 99 
                 1 
                 6 
               
               
                 80 
                 4 
                 99 
                 1 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 29. Synthesis of Oligonucleotide 135 (All-(Rp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108)) 
     Oligonucleotide 135 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 17.50 min. UPLC/ESI-MS: Calcd for C 186 H 278 N 51 O 73 P 11 S 11 : 5090.0; Found: 5091.9. 
     Example 30. Synthesis of Oligonucleotide 136 (All-(Sp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108)) 
     Oligonucleotide 136 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 19.25 min. UPLC/ESI-MS: Calcd for C 186 H 278 N 51 O 73 P 11 S 11 : 5090.0; Found: 5090.8. 
     Example 31. Synthesis of Oligonucleotide 137 ((Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) (1S-9R-1S)) 
     Oligonucleotide 137 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 17.85 min. UPLC/ESI-MS: Calcd for C 186 H 278 N 51 O 73 P 11 S 11 : 5090.0; Found: 5091.9. 
     Example 32. Synthesis of Oligonucleotide 138 ((Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) (2S-7R-2S)) 
     Oligonucleotide 138 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 18.10 min. UPLC/ESI-MS: Calcd for C 186 H 278 N 51 O 73 P 11 S 11 : 5090.0; Found: 5091.9. 
     Example 33. Synthesis of Oligonucleotide 139 ((Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) (1R-9S-1R)) 
     Oligonucleotide 139 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 18.75 min. UPLC/ESI-MS: Calcd for C 186 H 278 N 51 O 73 P 11 S 11 : 5090.0; Found: 5088.9. 
     Example 34. Synthesis of Oligonucleotide 140 ((Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) (2R-7S-2R)) 
     Oligonucleotide 140 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 18.72 min. UPLC/ESI-MS: Calcd for C 186 H 278 N 51 O 73 P 11 S 11 : 5090.0; Found: 5091.3. 
     Example 35. Synthesis of Oligonucleotide 141 ((Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) (3S-5R-3S)) 
     Oligonucleotide 141 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 18.09 min. UPLC/ESI-MS: Calcd for C 186 H 278 N 51 O 73 P 11 S 11 : 5090.0; Found: 5090.9. 
     Example 36. Synthesis of Oligonucleotide 142 ((Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) (3R-5S-3R)) 
     Oligonucleotide 142 was synthesized as described above. RT in RP-HPLC: 18.35 min. UPLC/ESI-MS (HPLC method 1): Calcd for C 186 H 278 N 51 O 73 P 11 S 11 : 5090.0; Found: 5088.9. 
     Example 37. Synthesis of Oligonucleotide 143 ((Sp, Sp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Sp, Sp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) ((SSR) 3 -SS)) 
     Oligonucleotide 143 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 18.48 min. UPLC/ESI-MS: Calcd for C 186 H 278 N 51 O 73 P 11 S 11 : 5090.0; Found: 5092.0. 
     Example 38. Synthesis of Oligonucleotide 144 ((Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp)-d[5mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1G] (SEQ ID NO: 108) ((RRS) 3 -RR)) 
     Oligonucleotide 144 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 18.02 min. UPLC/ESI-MS: Calcd for C 186 H 278 N 51 O 73 P 11 S 11 : 5090.0; Found: 5091.4. 
     Example 39. Synthesis of Oligonucleotide 145 (All-(Rp)-d[5mCs1Ts15mCs1As1Gs1Ts15mCs1Ts1Gs15mCs1Ts1Ts15mCs1Gs15mC] (SEQ ID NO: 108)) 
     Oligonucleotide 145 was synthesized as described above. RT in RP-HPLC (HPLC method 1): 17.30 min. UPLC/ESI-MS: Calcd for C 234 H 352 N 62 O 92 P 14 S 14 : 6388.3; Found: 6390.6. 
     Example 40. Synthesis of Oligonucleotide 146 (All-(Rp)-d[Gs15mCs1Ts1G]) 
     Oligonucleotide 146 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 15.89 min. UPLC/ESI-MS: Calcd for C 58 H 85 N 18 O 23 P 3 S 3 : 1591.5; Found: 1590.8. 
     Example 41. Synthesis of Oligonucleotide 147 (All-(Rp)-d[5mCs1As1Gs1T]) 
     Oligonucleotide 147 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 16.30 min. UPLC/ESI-MS: Calcd for C 58 H 85 N 18 O 22 P 3 S 3 : 1575.5; Found: 1575.2. 
     Example 42. Synthesis of Oligonucleotide 148 (All-(Rp)-d[5mCs2As2Gs2Ts25mCs2Ts2Gs25mCs2Ts2Ts25mCs2G] (SEQ ID NO: 108)) 
     Oligonucleotide 148 was synthesized as described above, using the sulfurization reagent in Example 22. RT in RP-HPLC (HPLC method 3): 16.51 min. UPLC/ESI-MS: Calcd for C 153 H 223 N 40 O 73 P 11 S 11 : 4484.1; Found: 4483.0. 
     Example 43. Synthesis of Oligonucleotide 149 (All-(Rp)-d[5mCs4As4Gs4Ts45mCs4Ts4Gs45mCs4Ts4Ts45mCs4G] (SEQ ID NO: 108)) 
     Oligonucleotide 149 was synthesized as described above, using the sulfurization reagent in Example 24. RT in RP-HPLC (HPLC method 4): 17.87 min. UPLC/ESI-MS: Calcd for C 252 H 388 N 51 O 95 P 11 S 11 : 6345.6; Found: 6346.5. 
     Synthesis of Chirally Controlled Chimeric Oligonucleotides Comprising Different Internucleotidic Linkages 
     As generally described above and herein, in some embodiments, the present invention provides chirally controlled oligonucleotide comprising different internucleotidic linkages. The non-limiting examples below illustrate such chirally controlled oligonucleotides, and the methods of synthesizing the same. 
     Examples 44-45 illustrate the synthesis of chirally controlled chimeric oligonucleotide comprising different internucleotidic linkages. The oligonucleotides containing mixed diastereomerically pure morpholinoethyl phosphorothioate triester/phosphorothioate diester internucleotidic linkages were synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-12 using 1 μmol synthesis column and 1.5 μmol of oxalyl linked dT on HCP. As each iterative cycle allows for a different sulfurization reagent to be reacted, two different thiosulfonates were used. The synthesis was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry solid support was then treated with 5 mL of anhydrous 1 M solution of 1,5-diazabicyclo(4.3.0)non-5-ene (DBN) in dry ACN-trimethylsilyl chloride—16:1 (v/v) for 10 min at r.t. The DBN solution was slowly pushed through the column by means of a plastic luer syringe fixed to the outlet of the column. The support was then washed with dry ACN and dried under vacuum. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product is purified by reverse phase preparative HPLC (Gradient of 5 to 65% ACN in 20 mM sodium phosphate buffer, pH=6.0). The fractions having purity above 95% are pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product is lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-12 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Examples 44-45. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                   
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 
                 20 
                 60 
               
               
                   
                   
                 16% NMI in THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-Morpholinoethyl 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                 1 
                 Toluylthiosulfonate 
                   
                 600  
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                   
                   
               
               
                   
               
               
                 6 
                 sulfurization 
                 0.3M S-cyanoethyl 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                 2 
                 Methylthiosulfonate 
                   
                 600  
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 44. Synthesis of Oligonucleotide 150 (All-(Rp)-d[TsCs1AsT]) 
     Oligonucleotide 150 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 12.72 min. UPLC/ESI-MS: Calcd for C 45 H 62 N 13 O 21 P 3 S 3 : 1310.2; Found: 1309.2 
     Example 45. Synthesis of Oligonucleotide 151 (All-(Sp)-d[Cs1AsGs1T]) 
     Oligonucleotide 151 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 14.71 min. UPLC/ESI-MS: Calcd for C 51 H 72 N 17 O 21 P 3 S 3 : 1448.4; Found: 1446.9. 
     Example 46 describes the synthesis of chirally controlled chimeric oligonucleotide comprising both phosphate diester internucleotidic linkage and modified internucleotidic linkages. The exemplary oligonucleotides containing mixed morpholinoethyl phosphorothioate triester/phosphodiester internucleotidic linkages were synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-13 using 1 μmol synthesis column and 1.5 μmol of oxalyl linked dT on HCP. As each iterative cycle allows for different sulfurization or oxidation reagents to be reacted, one thiosulfonate reagent was used for sulfurization in one cycle and iodine-promoted oxidation was used in another cycle. The synthesis was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product was purified by reverse phase preparative HPLC (Gradient of 5 to 65% ACN in 20 mM sodium phosphate buffer, pH=6.0). The fractions having purity above 95% were pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product was lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-13 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Example 46. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                   
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 
                 20 
                 60 
               
               
                   
                   
                 16% NMI in THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-Morpholinoethyl 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                   
                 Toluylthiosulfonate 
                   
                 600  
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                   
                   
               
               
                   
               
               
                 6 
                 oxidation 
                 0.02M I 2 , Pyridine/Water 
                 10 
                 300  
               
               
                   
               
            
           
         
       
     
     Example 46. Synthesis of Oligonucleotide 152 (All-(Sp)-d[Cs1AGs1T]) 
     Oligonucleotide 152 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 13.42 min. UPLC/ESI-MS: Calcd for C 51 H 72 N 17 O 22 P 3 S 2 : 1432.3; Found: 1431.7. 
     Example 47 describes the synthesis of chirally controlled chimeric oligonucleotide comprising a phosphodiester internucleotidic linkage and both modified chirally pure phosphotriester and phosphodiester internucleotidic linkages. The chirally controlled oligonucleotides containing mixed morpholinoethyl phosphorothioate triester/phosphodiester/phosphorothioate diester linkages were synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-14 using 1 μmol synthesis column and 1.5 μmol of oxalyl linked dT on HCP. As each iterative cycle allows for different sulfurization or oxidation reagents to be reacted, two different thiosulfonate reagents were used for the two different sulfurization cycles and iodine-promoted oxidation was used for another cycle. The synthesis was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry solid support was then treated with 5 mL of anhydrous 1 M solution of 1,5-diazabicyclo(4.3.0)non-5-ene (DBN) in dry ACN-trimethylsilyl chloride—16:1 (v/v) for 10 min at r.t. The DBN solution was slowly pushed through the column by means of a plastic luer syringe fixed to the outlet of the column. The support was then washed with dry ACN and dried under vacuum. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product was purified by reverse phase preparative HPLC (Gradient of 5 to 65% ACN in 20 mM sodium phosphate buffer, pH=6.0). The fractions having purity above 95% were pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product was lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-14 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA 
               
               
                 Synthesizer ABI-394 Used for the Synthesis of Example 47. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                 wait time 
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                  60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 
                 20 
                  60 
               
               
                   
                   
                 16% NMI in THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-cyanoethyl 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                 1 
                 MethylThioSulfonate 
                   
                 600 
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                   
                   
               
               
                   
               
               
                 6 
                 sulfurization 
                 0.3M S-Morpholinoethyl 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                 2 
                 ToluylThioSulfonate 
                   
                 600 
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                   
                   
               
               
                   
               
               
                 7 
                 oxidation 
                 0.02M I 2 , Pyridine/Water 
                 10 
                 300 
               
               
                   
               
            
           
         
       
     
     Example 47. Synthesis of Oligonucleotide 153 (All-(Sp)-d[CAs1GsT]) 
     Oligonucleotide 153 was synthesized as described above. RT in RP-HPLC (HPLC method 2): 11.48 min. UPLC/ESI-MS: Calcd for C 45 H 61 N 16 O 21 P 3 S 2 : 1318.1; Found: 1318.1. 
     As will be appreciated by a person skilled in the art, following examples 46 and 47, and other examples and methods described herein, longer chimeric chirally controlled oligonucleotides can be prepared. 
     Chirally Pure Oligonucleotides have Different Properties than the Mixture of Diastereomers from Non-Stereospecific Synthesis 
     As described above and herein, in some embodiments, the present application provides chirally pure oligonucleotides that have different chemical and biological properties than the mixture of diastereomers with the same base sequence but synthesized through non-stereospecific oligonucleotide synthesis. 
     Example 48. HPLC Profile of Chirally Pure Oligonucleotides and Mixture of Diastereomers from Non-Stereospecific Synthesis 
     Chirally pure phosphorothioate diester oligonucleotides A (Full R P , Oligonucleotide 101) and B (Full S P , Oligonucleotide 102) and non-stereospecific C (stereorandom phosphorothioate diester internucleotidic linkages), which was made by standard non-stereospecific oligonucleotide synthesis, were analyzed by the same RP-HPLC conditions and the corresponding HPLC traces are represented on  FIG.  2   . 
     As clearly demonstrated in  FIG.  2   , the stereochemistry of the phosphorothioate diester 20-mer oligonucleotides affects their behavior as determined by RP-HPLC and IEX-HPLC. Without the intention to be limited by theory, a correlation is observed between the retention times (RT) obtained by RP-HPLC and IEX-HPLC, as RT trends are conserved. The full R P  stereoisomer (A) has a shorter retention than the full S P  stereoisomer (B), while the stereorandom phosphorothioate diester oligonucleotide (C), being a mixture of all 2 19  stereoisomers, has a broad HPLC peak, eluting between the extreme full R P  and full S P  diastereoisomers. 
     As will be appreciated by those skilled in the art, the presented data herewith confirm that depicted analyses comparing different chirally controlled or uncontrolled (e.g., stereorandom) oligonucleotide compositions of the same sequence show that the exemplified chirally uncontrolled oligonucleotide composition (i.e., as prepared by non-chiral controlled oligonucleotide synthesis), includes only an extremely low level of certain oligonucleotide types, such as the full Rp or Sp type. 
     Example 49. Thermal Denaturation Experiment (Tm) 
     Each DNA strand was mixed with complementary RNA in equimolar concentration of 0.5 μM in 1×PBS. Total 2.5 mL solution was prepared for each duplex and the mixture was heated at 90° C. for 2 min and was allowed to cool down over the course of several hours. The mixtures were then stored at 4° C. for 2 hrs. Absorbance at 254 nm was recorded at an interval of 0.5 min starting the temperature gradient from 15° C. to 90° C. with rise of 0.5° C./minute, using a Perkin Elmer UV-spectrophotometer equipped with a Peltier unit. The 254 nm absorbance was plotted against the temperature and the Tm values were calculated from the respective first derivative of each curve. 
       FIG.  3    illustrates the difference in Tm between two stereopure diastereoisomer phosphorothioate oligonucleotides (full R P  20-mer A and full S P  20-mer B) and the stereorandom phosphorothioate oligonucleotide (C). Full R P  phosphorothioate DNA demonstrate higher affinity towards complementary RNA when compared to both the opposite full S P  diastereoisomer and the stereorandom 20-mer. 
     Table E-15 below summarizes the differences between chirally controlled oligonucleotides and stereorandom oligonucleotide. 
     
       
         
           
               
             
               
                 TABLE E-15 
               
             
            
               
                   
               
               
                 Differences between chirally controlled 
               
               
                 and stereorandom oligonucleotides. 
               
            
           
           
               
               
               
               
            
               
                   
                 RT-IEX 
                   
                 IC 50  (nM) (rounded 
               
               
                 Oligonucleotide 
                 (min) 
                 Tm (° C.) ± 0.5 
                 up to single digit) 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 101 
                 14.70 
                 68.3 
                 4 
               
               
                 102 
                 15.49 
                 60.5 
                 6 
               
               
                 103 
                 15.10 
                 63.4 
                 6 
               
               
                 104 
                 15.04 
                 63.5 
                 4 
               
               
                 105 
                 14.75 
                 66.5 
                 3 
               
               
                 106 
                 15.43 
                 61.8 
                 4 
               
               
                 107 
                 15.02 
                 64.5 
                 4 
               
               
                 108 
                 15.10 
                 64.8 
                 3 
               
               
                 109 
                 14.91 
                 65.8 
                 2 
               
               
                 110 
                 15.24 
                 62.9 
                 7 
               
               
                 111 
                 15.69 
                 NA 
                 NA 
               
               
                 113 
                 15.72 
                 NA 
                 NA 
               
               
                 114 
                 14.14 
                 65.2 
                 5 
               
               
                 115 
                 14.30 
                 65.2 
                 3 
               
               
                 116 
                 14.17 
                 64.8 
                 2 
               
               
                 118 
                 15.04 
                 64.5 
                 3 
               
               
                 119 
                 6.90 
                 73.4 
                 Not detected 
               
               
                 120 
                 15.49 
                 65.3 
                 4 
               
               
                   
               
            
           
         
       
     
     Example 50. Biological Activity of Chirally Pure Oligonucleotides 
     After checking OD all oligonucleotide candidate molecules were diluted to a starting concentration of 20 μM. Multidose forward transfection experiment was setup in 96-well plates using Hep3B cells (ATCC®, Cat. HB-8064™) using Lipofectamine 2000 (Life Technologies, Cat. 11668-019) transfection reagent. 
     Transfection Protocol: 
     2×10 4  Hep3B cells per well were seeded and incubated for 24 hours at 37° C. in a CO 2  incubator in 100 μl of antibiotic free MEM medium (Life Technologies, Cat. 11095098) containing 10% FBS and 1% Glutamax I (Life Technologies, Cat. 35050061). Twelve dilutions were setup in nuclease free water (Table E-16). 
     
       
         
           
               
             
               
                 TABLE E-16 
               
               
                   
               
               
                 Concentrations of oligonucleotide transfection stock plate. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                   
                 Dilution 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 1 
                 2 
                 3 
                 4 
                 5 
                 6 
               
               
                   
               
               
                 In μM 
                 20 
                 10 
                 3.33333 
                 1.11111 
                 0.37037 
                 0.12346 
               
               
                   
               
            
           
           
               
               
            
               
                   
                 Dilution 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 7 
                 8 
                 9 
                 10 
                 11 
                 12 
               
               
                   
               
               
                 In μM 
                 0.04115 
                 0.01372 
                 0.00457 
                 0.00152 
                 0.00051 
                 0.00017 
               
               
                   
               
            
           
         
       
     
     For each well, 0.5 μL of Lipofectamine 2000 were mixed with 9.5 μL of Opti MEM I reduced serum medium (Life Technologies, Cat. 31985062) and incubated for 5 minutes. Following incubation, 10 μL of each of the candidates at the reported concentrations was mixed with the diluted Lipofectamine 2000 by gentle pipetting. The mixture was then incubated for 20 minutes at room temperature to allow for complex formation. During this time the cell growth medium was replaced with 80 μL of fresh antibiotic free MEM as previously described. 20 μL of lipid-oligo complex was gently mixed into each well bringing the total volume to 100 μL. The cells were then incubated for 24 hours at 37° C. in a CO 2  incubator. 
     RNA Extraction: 
     After 24 hour incubation the cell growth medium was removed and RNA was extracted using a Dynabeads mRNA Direct kit (Life Technologies, Cat. 61012) per the instructions provided in the kit manual without modifications. This magnetic Oligo (dT) 25  bead system allows for cost-effective and robust high-throughput poly (A) RNA extraction circumventing DNAse treatment. RNA was eluted in nuclease-free water and stored at −80° C. 
     cDNA Synthesis: 
     10 μL of RNA was used in a 20 μL cDNA synthesis reaction using a High Capacity cDNA Reverse Transcription Kit (Life Technologies, Cat. 4374967) using the kit protocol with RNAse Inhibitor. The reverse transcription was performed in a 96-well format on a C1000 Touch thermal cycler (Biorad, Cat. 185-1196EDU). 
     Gene Expression Analysis: 
     Gene expression was measured by quantitative PCR using a LightCycler® 480 SYBR Green I Master (Roche, Cat. 04707516001) on LightCycler 480 Real-Time PCR instrument. Primers for the  Homo Sapiens  sequences of the target Apolipoprotein B (Apo B) (NM_000384) and endogenous control Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (NM_002046) were designed and ordered from IDT (Table E-17). 
     
       
         
           
               
             
               
                 TABLE E-17 
               
             
            
               
                   
               
               
                 Sequences of HPLC purified primers 
               
               
                 used for gene expression quantification. 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Primer 
                   
                   
                 SEQ ID 
                   
               
               
                   
                 Name 
                 Target 
                 Sequence 
                 NO: 
                 Position 
               
               
                   
               
               
                   
                 Apo B 
                   H.   sapiens   
                 AGCCTTGGTG 
                 116 
                 3209-3232 
               
               
                   
                 Forward 
                 Apo B 
                 GATACCCTGA 
                   
                   
               
               
                   
                 primer 
                   
                 AGTT 
                   
                   
               
               
                   
               
               
                   
                 Apo B 
                   H.   sapiens   
                 TGGACAAGGT 
                 117 
                 3289-3312 
               
               
                   
                 Reverse 
                 Apo B 
                 CATACTCTGC 
                   
                   
               
               
                   
                 primer 
                   
                 CGAT 
                   
                   
               
               
                   
               
               
                   
                 GAPDH 
                   H.   sapiens   
                 CTCTGCTCCT 
                 118 
                 30-49 
               
               
                   
                 Forward 
                 GAPDH 
                 CCTGTTCGAC 
                   
                   
               
               
                   
                 primer 
                   
                   
                   
                   
               
               
                   
               
               
                   
                 GAPDH 
                   H.   sapiens   
                 ACGACCAAAT 
                 119 
                 122-141 
               
               
                   
                 Reverse 
                 GAPDH 
                 CCGTTGACTC 
                   
                   
               
               
                   
                 primer 
               
               
                   
               
            
           
         
       
     
     Measurement of the target and endogenous control was performed in separate wells using material from the same cDNA template. SYBR green assay PCR conditions were setup as described in the SYBR Green I Master protocol (Table E-18). 
     
       
         
           
               
             
               
                 TABLE E-18 
               
               
                   
               
               
                 Real-time PCR conditions for SYBR green assay. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Step 
                 Number of Cycles 
               
               
                   
                   
               
               
                   
                 Pre-incubation 
                 1 
               
               
                   
                 Amplification 
                 45 
               
               
                   
                 Melting curve 
                 1 
               
               
                   
                 Cooling 
                 1 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                 Acquisition 
                   
                 Acquisition per 
               
               
                 Degrees ° C. 
                 Time 
                 Mode 
                 Ramp rate ° C./s 
                 ° C. 
               
               
                   
               
            
           
           
               
            
               
                 Pre-incubation 
               
            
           
           
               
               
               
               
               
               
            
               
                 95 
                 5 
                 min 
                 None 
                 4.4 
                 None 
               
            
           
           
               
            
               
                 Amplification 
               
            
           
           
               
               
               
               
               
               
            
               
                 95 
                 10 
                 sec 
                 None 
                 4.4 
                 None 
               
               
                 60 
                 10 
                 sec 
                 None 
                 2.2 
                 None 
               
               
                 72 
                 10 
                 sec 
                 Single 
                 4.4 
                 None 
               
            
           
           
               
            
               
                 Melting Curve 
               
            
           
           
               
               
               
               
               
               
            
               
                 95 
                 5 
                 sec 
                 None 
                 4.4 
                 None 
               
               
                 65 
                 1 
                 min 
                 None 
                 2.2 
                 None 
               
            
           
           
               
               
               
               
               
            
               
                 97 
                   
                 Continuous 
                 0.11 
                 5 
               
            
           
           
               
            
               
                 Cooling 
               
            
           
           
               
               
               
               
               
               
            
               
                 40 
                 30 
                 sec 
                 None 
                 2.2 
                 None 
               
               
                   
               
            
           
         
       
     
     Melt Curve Analysis show single amplicon peaks for each primer pair ( FIG.  4   ). 
     The data is a result of 3 biological replicates. All samples were compared to Naïve untransfected control. The following results were observed when evaluating gene expression by real-time PCR using SYBR green (Table E-19,  FIG.  5   ). 
     
       
         
           
               
             
               
                 TABLE E-19 
               
             
            
               
                   
               
               
                 Complete IC 50  data evaluated by SYBR green. 
               
            
           
           
               
               
               
            
               
                   
                 Oligonucleotide 
                 IC 50  (nM) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 101 
                 4.368 
               
               
                   
                 All-R 
               
               
                   
                 102 
                 6.345 
               
               
                   
                 All-S 
               
               
                   
                 103 
                 5.727 
               
               
                   
                 5R-9S-5R 
               
               
                   
                 104 
                 4.291 
               
               
                   
                 5S-9R-5S 
               
               
                   
                 105 
                 2.877 
               
               
                   
                 1S-17R-1S 
               
               
                   
                 106 
                 ~3.855 
               
               
                   
                 1R-17S-1R 
               
               
                   
                 107 
                 ~4.180 
               
               
                   
                 (R/S) 9 R 
               
               
                   
                 108 
                 3.189 
               
               
                   
                 (S/R) 9 S 
               
               
                   
                 118 
                 3.087 
               
               
                   
                 Stereorandom 
               
               
                   
                 109 
                 1.722 
               
               
                   
                 3S-13R-3S 
               
               
                   
                 110 
                 7.437 
               
               
                   
                 3R-13S-3R 
               
               
                   
                 114 
                 5.234 
               
               
                   
                 3R-13S-3R 
               
               
                   
                 115 
                 2.689 
               
               
                   
                 S-(RRS) 6   
               
               
                   
                 116 
                 1.919 
               
               
                   
                 RS-(RRS) 5 -RR 
               
               
                   
                 120 
                 4.386 
               
               
                   
                 Mipomersen 
               
               
                   
                   
               
            
           
         
       
     
     In some embodiments, provided chirally controlled oligonucleotides show an IC 50  below 8 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  below 7 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  below 6 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  below 5 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  below 4 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  below 3 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  below 2 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range of 0.5-8 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range of 1-4 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range of 1.5-3 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range of 1.5-2 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range of 0.5-2 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range of 1-2.5 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range of 1.5-3 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range of 2.5-5 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range of 3-6 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range of 5-8 nM. In some embodiments, provided chirally controlled oligonucleotides show an IC 50  within a range between an upper boundary and a lower boundary. In some such embodiments, the upper boundary is 8, 5, 4, or 3 nM. In some such embodiments, the lower boundary is 1, 1.5, 2, or 2.5 nM. As can be seen with reference to the Examples, the present disclosure specifically exemplifies various chirally controlled oligonucleotides or chirally controlled oligonucleotide compositions showing representative such IC 50  values. 
     Table E-19 illustrates that when test in chirally controlled form, certain chirally controlled oligonucleotides/oligonucleotide compositions are more potent than the corresponding stereorandom oligonucleotide mixture (Oligonucleotides 105, 109, 115 and 116 compared to Oligonucleotide 118); they are also more active than Mipomersen, which is a stereorandom oligonucleotide mixture (Oligonucleotides 104, 105, 106, 107, 109, 115 and 116 compared to 120). 
     Additional Chirally Controlled Preparations 
     Example 51. Preparation of Chirally Controlled Preparations of Oligonucleotides 
     
       
         
         
             
             
         
       
     
     The present Example describes preparation of a variety of particular chirally controlled composition of certain oligonucleotides as described herein. In particular, the present Example describes oligonucleotide preparation on utilizing loaded lcaa-CPG-500. 
     N 4 -Bz-5′-O-DMTr-2′-O-MOE-5mC (1) (4.0 g, 5.5 mmol) was dissolved in anhydrous DCM (20 mL) and mixed with 2 equiv of succinic anhydride (1.1 g, 11.1 mmol) and 3 equiv of 4-N,N-dimethylaminopyridine (2.0 g, 16.6 mmol). The reaction was stirred under Argon at room temperature. After complete consumption of the starting material as determined by TLC (1 hour), the solvents were evaporated to dryness, the crude residue was dissolved in DCM containing 1% triethylamine then purified by flash silica gel chromatography using a gradient of 0-2% of MeOH in DCM containing 2% triethylamine. Yield of pure compound (2) after evaporation was 4.5 g, 88%. The resulting 3′-O-succinate (2) (0.92 g, 1.0 mmol), N,N-diisopropylethylamine (0.82 ml, 5.0 mmol) and CPG-500 (10 g) were taken up in DMF (50 mL) then HBTU (0.42 g, 1.1 mmol) was added. The mixture was shaken for 2 h then filtered. The support was washed with DMF, MeOH and finally, DCM then dried in vacuo. Trityl cation analysis (monitoring at 504 nm) showed that the loading of the nucleoside on the support (3) was 63 μmol/g. 
     Chirally controlled compositions of oligonucleotides containing stereodefined chimeric deoxy and 2′-O-MOE phosphorothioate triester internucleotidic linkages was synthesized on MerMade-12 DNA/RNA synthesizer (BioAutomation) according to the cycle summarized in Table E-20 using a MM-6-200 synthesis column (BioAutomation) loaded with 2.0 g (126 μmol) of uncapped succinyl linked N 4 -Bz-5′-O-DMTr-2′-O-MOE-5mC (63 μmol/g, lcaa CPG-500 from Glen Research. The oligonucleotide synthesis cycle was performed with a preliminary capping step (capping 2) and without removal of the final terminal 5′-O-DMTr oligonucleotide group (DMT On). The stereospecific sulfurization steps were performed using the 0.3 M S-(2-cyanoethyl)methylthiosulfonate reagent following the coupling of the corresponding chiral phosphoramide and the two-step capping process (Table E-20). 
     Once the automated oligonucleotide synthesis cycle was completed with the final 5′-O-DMTr group kept On, the synthesis column was taken off the DNA/RNA synthesizer and dried under vacuum. The dried support was transferred onto an empty glass manual peptide synthesizer and 40 mL solution of 0.5 M 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 0.25 M N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) in ACN was continuously passed through the support for 5 min without stopping the flow in the manual peptide synthesizer. The support was washed by 50% Py/ACN and dried with Argon flow for 1 sec. Then, the support was transferred into an empty screw-cap plastic vial and treated with 5% EtOH/conc NH 3  (20 mL) at 60° C. for 6 h, and left at room temperature for 12 h. The support was removed by filteration and washed with conc. NH 3 . The filtrate was dried in vacuo then the resultant was dissolved in 50 mM TEAA (120 mL). Further filtration was performed when there was a suspension in the solution. The solution was loaded on a Sep-Pak cartridge (Waters, Sep-Pak Vac 35 cc (10 g) Cis Cartridges). The cartridge was washed with 20% ACN/50 mM TEAA (70 mL) to remove all capped truncated sequences and 50% ACN/water containing 0.5% of conc NH 3  (50 mL) to elute the full length DMT On oligonucleotide. The solution containing the DMT On oligonucleotide was dried in vacuo then diluted with 50 mM TEAA (120 mL) and loaded on another Sep-Pak cartridge (Waters, Sep-Pak Vac 35 cc (10 g) C 18  Cartridges). The cartridge was washed with milli Q water (50 mL), 2% TFA/water (50 mL), then water (50 mL). The DMT Off oligonucleotide was eluted with 50% ACN/water containing 0.5% of cone NH 3  (50 mL). 
     
       
         
           
               
             
               
                 TABLE E-20 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer 
               
               
                 MerMade 12 Used for Chirally Controlled Synthesis. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery  
                 wait 
               
               
                   
                   
                   
                 volume 
                 time 
               
               
                 step 
                 reaction 
                 reagent 
                 (mL) 
                 (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 × 10  
                 3 × 24  
               
               
                 2 
                 coupling 
                 0.15M chiral 
                 2 × 3.4 
                 2 × 450 
               
               
                   
                   
                 phosphoramidite in ACN + 
                   
                 (MOE) 
               
               
                   
                   
                 1.2M CMPT in ACN 
                   
                 2 × 300 
               
               
                   
                   
                   
                   
                 (DNA) 
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6- 
                 8 
                 60 
               
               
                   
                   
                 lutidine 
                   
                   
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6- 
                   6.8 
                 60 
               
               
                   
                   
                 lutidine + 16% NMI in THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-(2-cyanoethyl) 
                 9 
                 600  
               
               
                   
                   
                 methanethiosulfonate 
                   
                   
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Synthesis of Oligonucleotide ONT-75 (All (Rp))- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) 
     Synthesis of Oligonucleotide ONT-80 (All (Sp))- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) 
     Synthesis of Oligonucleotide ONT-77 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) (5R-10S-4R) 
     Synthesis of Oligonucleotide ONT-81 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) (5S-10R-4S) 
     Synthesis of Oligonucleotide ONT-87 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) (5R-(SSR) 3 -5R) 
     Synthesis of Oligonucleotide ONT-88 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) (5S-(RRS) 3 -5S) 
     Synthesis of Oligonucleotide ONT-89 (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC  (SEQ ID NO: 106) ((SR) 9 S) 
     Synthesis of Oligonucleotide ONT-82 (All (Rp))- GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC (SEQ ID NO: 120)   
     Synthesis of Oligonucleotide ONT-84 (All (Sp))- GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC (SEQ ID NO: 120)   
     Synthesis of Oligonucleotide ONT-85 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp)- GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC (SEQ ID NO: 120)  (5R-10S-4R) 
     Synthesis of Oligonucleotide ONT-86 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp)- GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC (SEQ ID NO: 120)  (5S-10R-4S) 
     Example 52: General RP-HPLC Method for the Analysis of Crude DMT On and Purified DMT Off Oligonucleotides 
     The present Example describes RP-HPLC analysis of crude and purified oligonucleotide compositions prepared by chirally controlled synthesis as described herein. 
     Buffer A: 50 mM TEAA, pH 7.0 
     Buffer B: ACN 
     Column: XBridge C 18 , 3.5 μm, C 18 , 4.6×50 mm, Part #186003034 
     Column temperature=50° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time(min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 99 
                 1 
                   
               
               
                 2 
                 1 
                 99 
                 1 
                 6 
               
               
                 52 
                 1 
                 50 
                 50 
                 6 
               
               
                 55 
                 1 
                 5 
                 95 
                 6 
               
               
                 55.5 
                 1 
                 5 
                 95 
                 6 
               
               
                 56 
                 1 
                 99 
                 1 
                 6 
               
               
                 60 
                 1 
                 99 
                 1 
                 1 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                 SEQ. 
                   
               
               
                   
                 ID 
                 FIG.  
               
               
                 RP-HPLC of Crude DMT on: 
                 NO: 
                 35: 
               
               
                   
               
             
            
               
                 ONT-75 (All (Rp))- Gs5mCs5mCsTs5mCs As 
                 106 
                 (Panel A) 
               
               
                 GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs 5mCs5mC   
                   
                   
               
               
                 ONT-80 (All (Sp))- Gs5mCs5mCsTs5mCs As 
                 106 
                 (Panel B) 
               
               
                 GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs 5mCs5mC   
                   
                   
               
               
                 ONT-77: (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, 
                 106 
                 (Panel C) 
               
               
                 Rp, Rp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs   
                   
                   
               
               
                   5mCs5mC  (5R-10S-4R) 
                   
                   
               
               
                 ONT-81 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp,  
                 106 
                 (Panel D) 
               
               
                 Sp,Sp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs   
                   
                   
               
               
                   5mCs5mC  (5S-10R-4S) 
                   
                   
               
               
                 ONT-87 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Rp, Rp, Rp,  
                 106 
                 (Panel E) 
               
               
                 Rp,Rp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs   
                   
                   
               
               
                   5mCs5mC  (5R-(SSR) 3 -5R) 
                   
                   
               
               
                 ONT-88 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, 
                 106 
                 (Panel F) 
               
               
                 Sp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs   
                   
                   
               
               
                   5mCs5mC  (5S-(RRS) 3 -5S) 
                   
                   
               
               
                 ONT-89 (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp,  
                 106 
                 (Panel G) 
               
               
                 Rp,Sp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs   
                   
                   
               
               
                   5mCs5mC  ((SR) 9 S) 
                   
                   
               
               
                 ONT-82 (All (Rp))- 
                 120 
                 (Panel H) 
               
               
                   GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC   
                   
                   
               
               
                 ONT-84 (All (Sp))- 
                 120 
                 (Panel I) 
               
               
                   GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC   
                   
                   
               
               
                 ONT-85 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp,  
                 120 
                 (Panel J) 
               
               
                 Rp,Rp)- GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC   
                   
                   
               
               
                 (5R-10S-4R) 
                   
                   
               
               
                 ONT-86 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp,  
                 120 
                 (Panel K) 
               
               
                 Sp,Sp)- GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC  (5S- 
                   
                   
               
               
                 10R-4S) 
               
               
                   
               
            
           
         
       
     
     Example 53: General IEX-HPLC Method for the Analysis of Crude DMT On and Purified DMT Off Oligonucleotides 
     The present Example describes IEX-HPLC analysis of crude and purified oligonucleotide compositions prepared by chirally controlled synthesis as described herein. 
     Buffer A: 10 mM TrisHCl, 50% ACN, pH 8.0 
     Buffer B: 10 mM TrisHCl, 800 mM NaClO 4 , 50% ACN, pH 8.0 
     Column: DIONEX, DNAPac, PA-100, Analytical, 4.0×250 mm, Part #063000 
     Column temperature=60° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 85 
                 15 
                   
               
               
                 3 
                 1 
                 85 
                 15 
                 1 
               
               
                 23 
                 1 
                 60 
                 40 
                 6 
               
               
                 25 
                 1 
                 5 
                 95 
                 6 
               
               
                 25.5 
                 1 
                 85 
                 15 
                 6 
               
               
                 30 
                 1 
                 85 
                 15 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 54: General UPLC-LCMS Method for the Analysis of Purified DMT Off Oligonucleotides 
     The present Example describes UPLC-LCMS analysis of purified oligonucleotide compositions prepared by chirally controlled synthesis as described herein. 
     Buffer A: 15 mM TEA, 400 mM HFIP, Water 
     Buffer B: 50:50 Buffer A/Methanol 
     Column: UPLC@OST C 18  1.7 μm, 2.1×500 mm 
     Column temperature=50° C. 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                 0.2 
                 80 
                 20 
                   
               
               
                 2 
                 0.2 
                 80 
                 20 
                 1 
               
               
                 22 
                 0.2 
                 30 
                 70 
                 6 
               
               
                 25 
                 0.2 
                 5 
                 95 
                 6 
               
               
                 25.5 
                 0.2 
                 80 
                 20 
                 6 
               
               
                 30 
                 0.2 
                 80 
                 20 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 55: General IEX-HPLC Method for the Purification of Crude DMT Off Oligonucleotide 
     The present Example describes IEX-HPLC purification of crude oligonucleotide compositions prepared by chirally controlled synthesis as described herein. 
     Buffer A: 20 mM NaOH, pH 11.0 
     Buffer B: 20 mM NaOH, 2.5 M NaCl, pH 11.0 
     Column: Empty column Waters AP-2 (Waters), custom in-house packed with Source 15Q support (GE Healthcare). Individual purification columns were packed and used for the different stereopure oligonucleotides. 
     Instrument: AKTA Purifier, equipped with the P-900 pump, the UPC-900 detector and the 50 mL injection SuperLoop (GE Healthcare) 
     Buffer heater temperature set=70° C. 
     Signal monitored at 254 nm 
     Fractions volume: 5 mL 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 100 
                 0 
                   
               
               
                 25 
                 10 
                 100 
                 0 
                 1 
               
               
                 40 
                 10 
                 85 
                 15 
                 6 
               
               
                 60 
                 10 
                 85 
                 15 
                 1 
               
               
                 80 
                 10 
                 70 
                 30 
                 6 
               
               
                 100 
                 10 
                 70 
                 30 
                 1 
               
               
                 140 
                 10 
                 60 
                 40 
                 6 
               
               
                 180 
                 10 
                 60 
                 40 
                 1 
               
               
                 200 
                 10 
                 45 
                 55 
                 6 
               
               
                 210 
                 10 
                 45 
                 55 
                 1 
               
               
                 211 
                 10 
                 100 
                 0 
                 6 
               
               
                 235 
                 10 
                 100 
                 0 
                 1 
               
               
                   
               
            
           
         
       
     
     Collected fractions were individually analyzed by analytical IEX-HPLC using the analytical conditions and gradient described above. Pure fractions were pooled in order to provide purified material of 95% and above purity as determined by the 254 nm UV absorbance profiles. 
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                 SEQ 
                   
               
               
                   
                 ID 
                 FIG. 
               
               
                 RP-HPLC of Purified DMT Off 
                 NO: 
                 36: 
               
               
                   
               
             
            
               
                 ONT-75 (All (Rp))- Gs5mCs5mCsTs5mCs As 
                 106 
                 (Panel A) 
               
               
                 GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs 5mCs5mC   
                   
                   
               
               
                 ONT-80 (All (Sp))- Gs5mCs5mCsTs5mCs As 
                 106 
                 (Panel B) 
               
               
                 GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs 5mCs5mC   
                   
                   
               
               
                 ONT-77 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, 
                 106 
                 (Panel C) 
               
               
                 Rp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs   
                   
                   
               
               
                   5mCs5mC  (5R-10S-4R) 
                   
                   
               
               
                 ONT-81 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, 
                 106 
                 (Panel D) 
               
               
                 Sp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs   
                   
                   
               
               
                   5mCs5mC  (5S-10R-4S) 
                   
                   
               
               
                 ONT-87 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, 
                 106 
                 (Panel E) 
               
               
                 Rp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs   
                   
                   
               
               
                   5mCs5mC  (5R-(SSR) 3 -5R) 
                   
                   
               
               
                 ONT-88 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, 
                 106 
                 (Panel F) 
               
               
                 Sp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs   
                   
                   
               
               
                   5mCs5mC  (5S-(RRS) 3 -5S) 
                   
                   
               
               
                 ONT-89 (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, 
                 106 
                 (Panel G) 
               
               
                 Sp)- Gs5mCs5mCsTs5mCs As GsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs   
                   
                   
               
               
                   5mCs5mC  ((SR) 9 S) 
                   
                   
               
               
                 ONT-82 (All (Rp))- 
                 120 
                 (Panel H) 
               
               
                   GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC   
                   
                   
               
               
                 ONT-84 (All (Sp))- 
                 120 
                 (Panel I) 
               
               
                   GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC   
                   
                   
               
               
                 ONT-85 (Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, 
                 120 
                 (Panel J) 
               
               
                 Rp)- GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC  (5R- 
                   
                   
               
               
                 10S-4R) 
                   
                   
               
               
                 ONT-86 (Sp, Sp, Sp, Sp, Sp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Rp, Sp, Sp, Sp, 
                 120 
                 (Panel K) 
               
               
                 Sp)- GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC  (5S- 
                   
                   
               
               
                 10R-4S) 
               
               
                   
               
            
           
         
       
     
     Table E-21. Compilation of the Physico-Chemical Properties for the Stereopure Phosphorothioate Oligonucleotides Synthesized as Described in Example XX. 
     Sequence A: human ApoB sequence 5′- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC -3′ (SEQ ID NO: 106). 
     Sequence B: mouse ApoB sequence 5′- GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC -3′ (SEQ ID NO: 120). 
     Underlined nucleotides designate 2′-O-MOE. s=phosphorothioate linkage. 5mC=5-methyl-2′-deoxycytidine.  5mC =5-methyl-2′-O-MOE-cytidine. Stereo architecture describes the stereoisomer nature (Rp/Sp) of each phosphorus atom in a given phosphorothioate linkage of the oligonucleotide. Retention time (t R ) in IEX-HPLC and Found molecular weight (MW) values were obtained using the corresponding analytical methods for the purified compounds (described above). 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                   
                   
                   
                 t R  IEX- 
                   
                   
               
               
                 sequence 
                   
                   
                 HPLC 
                 Calc 
                 Found 
               
               
                 ID 
                 sequence 
                 stereo architecture 
                 (min) 
                 MW 
                 MW 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 ONT-41 
                 A 
                 Stereorandom 
                 17.34 
                 7177.2 
                 7175.7 
               
               
                   
                   
                 diastereomixture 
               
               
                 ONT-75 
                 A 
                 All Rp 
                 16.44 
                 7177.2 
                 7177.0 
               
               
                 ONT-77 
                 A 
                 5R-10S-4R 
                 17.44 
                 7177.2 
                 7177.5 
               
               
                 ONT-80 
                 A 
                 All Sp 
                 18.13 
                 7177.2 
                 7175.5 
               
               
                 ONT-81 
                 A 
                 5S-10R-4S 
                 17.67 
                 7177.2 
                 7175.8 
               
               
                 ONT-87 
                 A 
                 5R-(SSR) 3 -5R 
                 13.64 
                 7177.2 
                 — 
               
               
                 ONT-88 
                 A 
                 5S-(RRS) 3 -5S 
                 14.88 
                 7177.2 
                 7177.0 
               
               
                 ONT-89 
                 A 
                 (SR) 9 S 
                 14.25 
                 7177.2 
                 — 
               
               
                 ONT-83 
                 B 
                 Stereorandom 
                 17.02 
                 7233.2 
                 7231.8 
               
               
                   
                   
                 diastereomixture 
               
               
                 ONT-82 
                 B 
                 All Rp 
                 16.43 
                 7233.2 
                 7231.6 
               
               
                 ONT-84 
                 B 
                 All Sp 
                 19.51 
                 7233.2 
                 7232.1 
               
               
                 ONT-85 
                 B 
                 5R-10S-4R 
                 18.76 
                 7233.2 
                 7231.1 
               
               
                 ONT-86 
                 B 
                 5S-10R-4S 
                 18.75 
                 7233.2 
                 7230.9 
               
               
                   
               
            
           
         
       
     
     Example 56: General RP-HPLC Method for the Overlay Analyses of Purified DMT Off Oligonucleotides 
     The present Example describes RP-HPLC analysis of purified oligonucleotide compositions prepared by chirally controlled synthesis as described herein. 
     Buffer A: 50 mM TEAA, pH 7.0 
     Buffer B: ACN 
     Column: XBridge C 18 , 3.5 μm, C 18 , 4.6×50 mm 
     Column temperature=50° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time(min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 95 
                 5 
                   
               
               
                 2 
                 1 
                 95 
                 5 
                 1 
               
               
                 52 
                 1 
                 70 
                 30 
                 6 
               
               
                 54 
                 1 
                 5 
                 95 
                 6 
               
               
                 54.5 
                 1 
                 5 
                 95 
                 1 
               
               
                 55 
                 1 
                 99 
                 1 
                 6 
               
               
                 60 
                 1 
                 99 
                 1 
                 1 
               
               
                   
               
            
           
         
       
     
     Panel A of  FIG.  37    is an overlay of RP-HPLC traces of Purified DMT Off Oligonucleotide ONT-75, ONT-77, ONT-80, ONT-81, ONT-87, ONT-88, ONT-89 and ONT-41 (diastereomixture)- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs 5mCs5mC  (SEQ ID NO: 106). 
     Panel B of  FIG.  37    shows an expanded view of Panel A, with each curve labeled as follows: 
     
       
         
           
               
               
             
               
                   
               
               
                 Curve # 
                 Oligonucleotide 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 1 
                 diastereomixture (ONT-41) 
               
               
                 2 
                 all-Rp (ONT-75) 
               
               
                 3 
                 5R-(SSR) 3 -5R (ONT-87) 
               
               
                 4 
                 (SR) 9 -S (ONT-89) 
               
               
                 5 
                 5R-10S-4R (ONT-77) 
               
               
                 6 
                 5S-10R-4S (ONT-81) 
               
               
                 7 
                 5S-(RRS) 3 -5S (ONT-88) 
               
               
                 8 
                 all-Sp (ONT-80) 
               
               
                   
               
            
           
         
       
     
     Panel A of  FIG.  38    is an overlay of RP-HPLC of Purified DMT Off Oligonucleotide ONT-82, ONT-84, ONT-85, ONT-86, and ONT-83 (diastereomixture)- GsTs5mCs5mCs5mCs TsGsAsAsGsAsTsGsTs5mCs AsAsTsGs5mC (SEQ ID NO: 120)   
     Panel B of  FIG.  38    shows an expanded view of Panel A, with each curve labeled as follows: 
     
       
         
           
               
               
             
               
                   
               
               
                 Curve # 
                 Oligonucleotide 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 1 
                 diastereomixture (ONT-83) 
               
               
                 2 
                 all-Rp (ONT-82) 
               
               
                 3 
                 5S-10R-4S (ONT-86) 
               
               
                 4 
                 5R-10S-4R (ONT-85) 
               
               
                 5 
                 all-Sp (ONT-84) 
               
               
                   
               
            
           
         
       
     
     Example 57: Thermal Denaturation Experiment (Tm) 
     The present Example describes characterization of chirally controlled oligonucleotide compositions using thermal denaturation. 
     Each DNA strand was mixed with its complementary RNA strand in equimolar concentration of 1 μM in 1×PBS. Total 3 mL solution was prepared for each duplex and the mixture was heated at 90° C. for 2 min and was allowed to cool down over the course of several hours. The mixtures were then stored at 4° C. for 2 hrs. Absorbance at 254 nm was recorded at an interval of 0.5 min starting the temperature gradient from 15.0° C. to 95.0° C. with rise of 0.5° C./minute, using Cary100 Series (Agilent Technologies). The 254 nm absorbance was plotted against the temperature and the Tm values were calculated from the respective first derivative of each curve. 
       FIG.  39    presents a Tm overlay of chirally controlled oligonucleotides and stereorandom oligonucleotide. 
       FIG.  39    illustrates the difference in Tm between four stereopure diastereoisomer phosphorothioate oligonucleotides (all-Rp 20-mer, all-Sp 20-mer, 5R-10S-4R and 5S-10R-4S gapmers) and the stereorandom phosphorothioate oligonucleotide. Full Rp phosphorothioate DNA demonstrates the highest affinity towards complementary RNA (Tm=85.1° C.) when compared to full Sp 20-mer (Tm=75.1° C.) which has the lowest affinity. 5R-10S-4R and 5S-10R-4S gapmers and the stereorandom phosphorothioate oligonucleotides all showed intermediates values (Tm range=80.1-81.2° C.). 
     Table E-22, below, gives Tm values for the studied stereopure and stereorandom phosphorothioate oligonucleotides having the human ApoB sequence 5′- Gs5mCs5mCsTs5mCs AsGsTs5mCsTsGs5mCsTsTs5mCs Gs5mCsAs5mCs5mC-3′  (SEQ ID NO: 106) with various stereochemistry architectures on the phosphorothioate backbone. 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE E-22 
               
               
                   
                   
               
               
                   
                 sequence ID 
                 stereo architecture 
                 Tm (° C.) 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 ONT-41 
                 Stereorandom 
                 81.0 
               
               
                   
                   
                 diastereomixture 
               
               
                   
                 ONT-75 
                 All Rp 
                 85.1 
               
               
                   
                 ONT-77 
                 5R-10S-4R 
                 80.1 
               
               
                   
                 ONT-80 
                 All Sp 
                 75.1 
               
               
                   
                 ONT-81 
                 5S-10R-4S 
                 81.2 
               
               
                   
                   
               
            
           
         
       
     
     Example 58: Preparation of Exemplary Chirally Controlled siRNA Oligonucleotides Targeting PCSK9 
     Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme involved in cholesterol metabolism. PCSK9 binds to the receptor for low density lipoprotein (LDL), triggering its destruction. Although LDL associated with the receptor is also eliminated when the receptor is destroyed, the net effect of PCSK9 binding in fact increases LDL levels, as the receptor would otherwise cycle back to the cell surface and remove more cholesterol. 
     Several companies are developing therapeutic agents that target PCSK9. Of particular relevance to the present disclosure, each of Isis Pharmaceuticals, Santaris Pharma, and Alnylam Pharmaceuticals is developing a nucleic acid agent that inhibits PCSK9. The Isis Pharmaceuticals product, an antisense oligonucleotide, has been shown to increase expression of the LDLR and decrease circulating total cholesterol levels in mice (Graham et al “Antisense inhibition of proprotein convertase subtilisin/kexin type 9 reduces serum LDL in hyperlipidemic mice”.  J. Lipid Res.  48 (4): 763-7, April 2007). Initial clinical trials with the Alnylam Pharmaceuticals product, ALN-PCS, reveal that RNA interference offers an effective mechanism for inhibiting PCSK9 (Frank-Kamenetsky et al “Therapeutic RNAi targeting PCSK9 acutely lowers plasma cholesterol in rodents and LDL cholesterol in nonhuman primates”.  Proc. Natl. Acad. Sci. U.S.A.  105 (33): 11915-20, August 2008). 
     The present Example describes preparation of a variety of chirally controlled or stereorandom siRNA agents directed to PCSK9. Specifically, this Example describes preparation of each of the following oligonucleotide compositions as illustrated in Table E-23 below: 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE E-23 
               
               
                   
               
               
                   
                   
                 SEQ 
                   
                   
               
               
                   
                   
                 ID 
                   
                   
               
               
                   
                 ONT # 
                 NO: 
                 Sequence 
                 Type 
               
               
                   
               
             
            
               
                   
                 ONT-106 
                 121 
                 (Rp)-uucuAGAccuGu 
                 PCSK9 
               
               
                   
                   
                   
                 uuuGcuudT  dT 
                 sense 
               
               
                   
               
               
                   
                 ONT-107 
                 121 
                 (Sp)-uucuAGAccuGu 
                 PCSK9 
               
               
                   
                   
                   
                 uuuGcuudT  dT 
                 sense 
               
               
                   
               
               
                   
                 ONT-108 
                 122 
                 (Rp)-AAGcAAAAcAGG 
                 PCSK9 
               
               
                   
                   
                   
                 UCuAGAAdT s dt 
                 antisense 
               
               
                   
               
               
                   
                 ONT-109 
                 122 
                 (Sp)-AAGcAAAAcAGG 
                 PCSK9 
               
               
                   
                   
                   
                 UCuAGAAdT  dT 
                 antisense 
               
               
                   
               
               
                   
                 ONT-110 
                 122 
                 (Rp,  R p)-asAGcAAA 
                 PCSK9 
               
               
                   
                   
                   
                 AcAGGUCuAGAAdT  dT 
                 antisense 
               
               
                   
               
               
                   
                 ONT-111 
                 123 
                 (Sp,  R p)-asGcAAAA 
                 PCSK9 
               
               
                   
                   
                   
                 cAGGUCuAGAAdT  dT 
                 antisense 
               
               
                   
               
               
                   
                 ONT-112 
                 123 
                 (Sp,  S p)-asGcAAAA 
                 PCSK9 
               
               
                   
                   
                   
                 cAGGUCuAGAAdT  dT 
                 antisense 
               
               
                   
               
               
                   
                 ONT-113 
                 123 
                 (Rp,  S p)-asGcAAAA 
                 PCSK9 
               
               
                   
                   
                   
                 cAGGUCuAGAAdT  dT 
                 antisense 
               
               
                   
               
               
                 NOTE: 
               
               
                 lower case letters represent 2′OMe RNA residues; 
               
               
                 capital letters represent 2′OH RNA residues; 
               
               
                 and bolded and italicized “s” indicates a phosphorothioate moiety. 
               
            
           
         
       
     
     Lcaa-CPG-500 used in the preparation of these oligonucleotides was prepared according to the following reaction: 
     
       
         
         
             
             
         
       
     
     In particular, 5′-O-DMTr-2′-deoxythymidine (1) (4.28 g, 7.86 mmol) was dissolved in anhydrous DCM (50 mL) and mixed with 2 equiv of succinic anhydride (1.57 g, 15.7 mmol) and 3 equiv of 4-N,N-dimethylaminopyridine (2.88 g, 23.6 mmol). The reaction was stirred under Argon at room temperature. After complete consumption of the starting material as determined by TLC (1 hour), the solvents were evaporated to dryness, the crude residue was dissolved in DCM containing 1% triethylamine then purified by flash silica gel chromatography using a gradient of 0-2% of MeOH in DCM containing 2% triethylamine. Yield of pure compound (2) after evaporation was 5.5 g, 94%. The resulting 5′-O-DMTr-2′-deoxythymidine-3′-O-succinate (2) (0.60 g, 0.81 mmol), N,N-diisopropylethylamine (0.71 mL, 4.02 mmol) and CPG-500 (10 g) were taken up in DMF (50 mL) then HBTU (0.37 g, 0.97 mmol) was added. The mixture was shaken for 2 h then filtered. The support was washed with DMF, MeOH and finally, DCM then dried in vacuo. Trityl cation analysis (monitoring at 504 nm) showed that the loading of nucleoside on the support (3) was 38 μmol/g. 
     Each oligonucleotide, containing 2′-OH and 2′-OMe phosphodiester and stereodefined 2′-deoxy and 2′-OMe phosphorothioate diester internucleotidic linkages as indicated above in Table E-23, was synthesized on an ABI 394 DNA/RNA synthesizer according to the cycles summarized in Table E-24 and Table E-25 respectively, using a 10 μmol capacity synthesis column loaded with 130 mg (4.9 μmol) of succinyl linked 5′-O-DMTr-2′-deoxythymidine (38 μmol/g). Prior to synthesis, a preliminary capping step (capping 2) was performed and the synthesis was terminated with removal of the terminal 5′-O-DMTr groups. The oxidation step was performed using a commercially available 5-6 M solution of tert-butyl hydroperoxide (TBHP) in decane which was then diluted with four parts dichloromethane. The stereospecific sulfurization step was performed using the 0.3 M S-cyanoethylmethylthiosulfonate reagent following the coupling of the corresponding chiral phosphoramide and the two-step capping process (Table E-25). 
     Once the automated oligonucleotide synthesis cycle was completed and the final 5′-O-DMTr group was removed, the synthesis column was taken off the DNA/RNA synthesizer and dried under vacuum. 10 mL solution of 0.5 M 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 0.25 M N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) in ACN was continuously added to the support through the synthesis column for 1 min without stopping the flow using a syringe attached to one end of the synthesis column. The support was then washed with anhydrous ACN and dried under vacuum. Then, the dried support was transferred into an empty screw-cap plastic vial and treated with 40% MeNH 2  aqueous solution (0.5 mL) at 60° C. for 10 min. After that, the vial was immediately cooled down and after dilution with DMSO (0.5 mL), the support was removed by filtration, washed again with DMSO (1 mL) and filtered. The filtrate was cooled down and then immediately treated with 3HF.Et 3 N (0.75 mL) at 60° C. for 10 min, then immediately frozen and stored at 4° C. prior to purification. 
     
       
         
           
               
             
               
                 TABLE E-24 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer 
               
               
                 ABI 394 (2′-O-TBDMS and 2′-OMe substituted RNA cycle) 
               
            
           
           
               
               
               
               
               
            
               
                 step 
                 reaction 
                 reagent 
                 delivery time (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 120 + 10 
                 N. A. 
               
               
                 2 
                 coupling 
                 0.15M 
                 7 + 6 
                 30 + 600 
               
               
                   
                   
                 phosphoramidite in 
               
               
                   
                   
                 ACN + 2M CMPT in 
               
               
                   
                   
                 ACN 
               
               
                 3 
                 capping 
                 5% Pac 2 O in THF/2,6- 
                 10 
                 20 
               
               
                   
                   
                 lutidine + 16% NMI in 
               
               
                   
                   
                 THF 
               
               
                 4 
                 oxidation 
                 1.1M tert-butyl 
                 20 
                 110 
               
               
                   
                   
                 hydroperoxide in 4:1 
               
               
                   
                   
                 dichloromethane:decane 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE E-25 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI 394 
               
               
                 (stereodefined phosphorothioate 2′-deoxy and 2′-OMe RNA cycle) 
               
            
           
           
               
               
               
               
               
            
               
                 step 
                 reaction 
                 reagent 
                 delivery time (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 120 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M chiral 
                 8 + 6 
                 30 + 900 (2′-OMe 
               
               
                   
                   
                 phosphoramidite in 
                   
                 RNA) 
               
               
                   
                   
                 ACN + 2M CMPT in 
                   
                 30 + 600 (DNA) 
               
               
                   
                   
                 ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6- 
                 30 
                 60 
               
               
                   
                   
                 lutidine 
                   
                   
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6- 
                 30 
                 60 
               
               
                   
                   
                 lutidine + 16% NMI in 
                   
                   
               
               
                   
                   
                 THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-(2-cyanoethyl) 
                 15 + 3 × 4 
                 120 + 3 × 60 + 300 
               
               
                   
                   
                 methylthiosulfonate 
                   
                   
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 59. General IEX-HPLC Method for the Analysis of Crude and Purified DMT Off RNA Oligonucleotides 
     Buffer A: 20 mM sodium phosphate, pH 11.0 
     Buffer B: 20 mM sodium phosphate, 1 M NaBr, pH 11.0 
     Column: DIONEX, DNAPac, PA-100, Analytical, 4.0×250 mm 
     Column temperature=60° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 95 
                 5 
                   
               
               
                 3 
                 1 
                 95 
                 5 
                 1 
               
               
                 23 
                 1 
                 20 
                 80 
                 6 
               
               
                 25 
                 1 
                 5 
                 95 
                 6 
               
               
                 25.5 
                 1 
                 95 
                 5 
                 6 
               
               
                 30 
                 1 
                 95 
                 5 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 60. General UPLC-LCMS Method for the Analysis of Purified DMT Off RNA Oligonucleotides 
     Buffer A: 15 mM TEA, 400 mM HFIP, Water 
     Buffer B: 50:50 Buffer A/Methanol 
     Column: UPLC@OST C 18  1.7 μm, 2.1×500 mm 
     Column temperature=50° C. 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                 0.2 
                 95 
                 5 
                   
               
               
                 10 
                 0.2 
                 35 
                 65 
                 6 
               
               
                 12 
                 0.2 
                 5 
                 95 
                 6 
               
               
                 12.5 
                 0.2 
                 95 
                 5 
                 6 
               
               
                 15 
                 0.2 
                 95 
                 5 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 61. General IEX-HPLC Method for the Purification of Crude DMTr Off RNA Oligonucleotides 
     Buffer A: 20 mM sodium phosphate, pH 8.5 
     Buffer B: 20 mM sodium phosphate, 1 M NaBr, pH 8.5 
     Column: Empty column Waters AP-2 (Waters), custom in-house packed with Source 15Q support (GE Healthcare). The same purification column was used for the different stereopure oligonucleotides. 
     Instrument: Waters HPLC unit equipped with the 2525 binary gradient module, the 2487 dual absorbance detector and the 20 mL Flex injector (Waters). 
     Buffer heater temperature set=70° C. 
     Signal monitored at 254 nm and 280 nm 
     Fractions volume: 4.5 mL 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 100 
                 0 
                   
               
               
                 10 
                 10 
                 100 
                 0 
                 1 
               
               
                 25 
                 10 
                 80 
                 20 
                 6 
               
               
                 85 
                 10 
                 67.5 
                 32.5 
                 6 
               
               
                 95 
                 10 
                 67.5 
                 32.5 
                 1 
               
               
                 155 
                 10 
                 55 
                 45 
                 6 
               
               
                 165 
                 10 
                 0 
                 100 
                 6 
               
               
                 170 
                 10 
                 100 
                 0 
                 6 
               
               
                 180 
                 10 
                 100 
                 0 
                 1 
               
               
                   
               
            
           
         
       
     
     Collected fractions were individually analyzed by analytical IEX-HPLC using the analytical conditions and gradient described above. Pure fractions were pooled in order to provide purified material of 95% and above purity as determined by the 254 nm UV absorbance profiles. 
     Example 62. General RP-Sep-Pak Method for the Desalting of Purified RNA Oligonucleotides 
     The solution of pooled pure fractions was loaded on a Sep-Pak cartridge (Waters, Sep-Pak Vac 35 cc (10 g) C 18  Cartridges) pre-conditioned with water. After loading of the sample (100 mL), the cartridge was washed with milli Q water (50 mL) to remove all salt and then washed with 50% ACN/water to elute the full length desalted RNA oligonucleotide. The collected solution was concentrated in vacuo to a volume of 5 mL and lyophilized from water. 
     Example 63: Preparation of Double Stranded siRNA Agents Using Chirally Controlled Oligonucleotide Strands 
     The present Example describes preparation of double stranded siRNA agents by thermal annealing of chirally controlled oligonucleotide strands as described above. 
     Each RNA strand was mixed with its complementary RNA strand in equimolar concentration of 10 μM in 1×PBS. Total 0.5 mL solution was prepared for each duplex and the mixture was heated at 90° C. for 2 min and was allowed to cool down over the course of several hours. The mixtures were then stored at 4° C. Physico-chemical properties of the utilized RNA strands are presented below in Table E-26. 
     
       
         
           
               
               
               
               
               
               
             
               
                 TABLE E-26 
               
               
                   
               
               
                 duplex 
                   
                 sequence 
                 stereo 
                 Calc 
                 Found 
               
               
                 ID 
                 sequence 
                 ID 
                 architecture 
                 MW 
                 MW 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 ONT D-1 
                 S 
                 ONT-116 
                 Stereorandom 
                 6735.4 
                 6734.9 
               
               
                   
                   
                   
                 diastereomixture 
               
               
                   
                 AS1 
                 ONT-114 
                 Stereorandom 
                 6805.4 
                 6805.2 
               
               
                   
                   
                   
                 diastereomixture 
               
               
                 ONT D-2 
                 S 
                 ONT-107 
                 Sp 
                 6735.4 
                 6736.6 
               
               
                   
                 AS1 
                 ONT-109 
                 Sp 
                 6805.4 
                 6801.6 
               
               
                 ONT D-3 
                 S 
                 ONT-106 
                 Rp 
                 6735.4 
                 6731.8 
               
               
                   
                 AS1 
                 ONT-109 
                 Sp 
                 6805.4 
                 6801.6 
               
               
                 ONT D-4 
                 S 
                 ONT-107 
                 Sp 
                 6735.4 
                 6736.6 
               
               
                   
                 AS1 
                 ONT-108 
                 Rp 
                 6805.4 
                 6799.2 
               
               
                 ONT D-5 
                 S 
                 ONT-106 
                 Rp 
                 6735.4 
                 6731.8 
               
               
                   
                 AS1 
                 ONT-108 
                 Rp 
                 6805.4 
                 6799.2 
               
               
                 ONT D-7 
                 S 
                 ONT-116 
                 Stereorandom 
                 6735.4 
                 6734.9 
               
               
                   
                   
                   
                 diastereomixture 
               
               
                   
                 AS2 
                 ONT-115 
                 Stereorandom 
                 6835.5 
                 6835.2 
               
               
                   
                   
                   
                 diastereomixture 
               
               
                 ONT D-8 
                 S 
                 ONT-106 
                 Rp 
                 6735.4 
                 6731.8 
               
               
                   
                 AS2 
                 ONT-110 
                 Rp, Rp 
                 6835.5 
                 6832.3 
               
               
                 ONT D-9 
                 S 
                 ONT-107 
                 Sp 
                 6735.4 
                 6736.6 
               
               
                   
                 AS2 
                 ONT-110 
                 Rp, Rp 
                 6835.5 
                 6832.2 
               
               
                 ONT D-10 
                 S 
                 ONT-106 
                 Rp 
                 6735.4 
                 6731.8 
               
               
                   
                 AS2 
                 ONT-111 
                 Sp, Rp 
                 6835.5 
                 6832.4 
               
               
                 ONT D-11 
                 S 
                 ONT-107 
                 Sp 
                 6735.4 
                 6736.6 
               
               
                   
                 AS2 
                 ONT-111 
                 Sp, Rp 
                 6835.5 
                 6832.4 
               
               
                 ONT D-12 
                 S 
                 ONT-106 
                 Rp 
                 6735.4 
                 6731.8 
               
               
                   
                 AS2 
                 ONT-112 
                 Sp, Sp 
                 6835.5 
                 6836.2 
               
               
                 ONT D-13 
                 S 
                 ONT-107 
                 Sp 
                 6735.4 
                 6736.6 
               
               
                   
                 AS2 
                 ONT-112 
                 Sp, Sp 
                 6835.5 
                 6836.2 
               
               
                 ONT D-14 
                 S 
                 ONT-106 
                 Rp 
                 6735.4 
                 6731.8 
               
               
                   
                 AS2 
                 ONT-113 
                 Rp, Sp 
                 6835.5 
                 6834.6 
               
               
                 ONT D-15 
                 S 
                 ONT-107 
                 Sp 
                 6735.4 
                 6736.6 
               
               
                   
                 AS2 
                 ONT-113 
                 Rp, Sp 
                 6835.5 
                 6834.6 
               
               
                   
               
            
           
         
       
     
     Oligonucleotide sequences used, human PCSK9 siRNA: Sense strand (S) 5′-uucuAGAccuGuuuuGcuudTsdT-3′ (SEQ ID NO: 121); Antisense strand 1 (AS1) 5′-AAGcAAAAcAGGUCuAGAAdTsdT-3′ (SEQ ID NO: 122); Antisense strand 2 (AS2) 5′-asAGcAAAAcAGGUCuAGAAdTsdT-3′ (SEQ ID NO: 123). Upper case nucleotides: RNA, lower case nucleotides 2′-OMe, d=2′-deoxy, s=phosphorothioate. Stereo architecture describes the stereoisomer nature (Rp/Sp absolute configuration) of each phosphorus atom in a given phosphorothioate linkage of the oligonucleotide. Found molecular weight (MW) values were obtained using the corresponding analytical methods for the purified compounds (described above). 
       FIG.  51    shows overlays of IEX-HPLC profiles showing difference in retention times between stereopure RNA oligonucleotides. 
     
       
         
           
               
               
             
               
                   
               
               
                 Curve # 
                 Oligonucleotide 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 1 
                 ONT-109 (Sp) 
               
               
                 2 
                 ONT-114 (diastereomixture) 
               
               
                 3 
                 ONT-108 (Rp) 
               
               
                   
               
            
           
         
       
     
       FIG.  52    shows overlays of IEX-HPLC profiles showing difference in retention times between stereopure RNA oligonucleotides. 
     
       
         
           
               
               
             
               
                   
               
               
                 Curve # 
                 Oligonucleotide 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 1 
                 ONT-109 (Rp) 
               
               
                 2 
                 ONT-114 (diastereomixture) 
               
               
                 3 
                 ONT-107 (Sp) 
               
               
                   
               
            
           
         
       
     
     In addition to the above specifically exemplified, prepared chirally controlled siRNA oligonucleotides, the present invention provides for preparation of siRNA duplexes having several chiral phosphorothioate internucleotide linkages, and full chiral phosphorothioate internucleotide linkages. 
     For example, in accordance with the present invention, multiple chiral phosphorothioate linkages are introduced inside a RNA oligonucleotide by using the appropriate chiral RNA 3′-phosphoramidites, having suitable 2′-OH protecting groups, such as 2′-O-PivOM (Debart et al.,  Chem. Eur. J.,  2008, 14, 9135), 2′-O-CEM (Ohgi et al.,  Org. Lett.,  2005, 7, 7913; Wada et al.,  J. Org. Chem.,  2012, 77, 7913), 2′-O-TOM (Pitsch et al.,  Helv. Chim. Acta,  2001, 84, 3773) or 2′-O-TC (Dellinger et al.,  J. Am. Chem. Soc.,  2011, 133, 11540). Each of the following Human PCSK9 siRNA Sense Strands having several chiral phosphorothioate internucleotide linkages and full chiral phosphorothioate internucleotide linkages can be prepared in accordance with the present invention: 
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                 SEQ 
                   
               
               
                   
                 ID 
                   
               
               
                   
                 NO: 
                 PCSK9 siRNA Sense Strands 
               
               
                   
               
             
            
               
                 PCSK9 
                 121 
                 (All (Sp))- 
               
               
                 (1) 
                   
                 ususcsusAsGsAscscsusGsususususGscsususdTsdT 
               
               
                 PCSK9 
                 121 
                 (All (Rp))- 
               
               
                 (2) 
                   
                 ususcsusAsGsAscscsusGsususususGscsususdTsdT 
               
               
                 PCSK9 
                 121 
                 (All (Sp))-usucuAsGsAsccuGsuuuuGscuusdTsdT 
               
               
                 (3) 
                   
                   
               
               
                 PCSK9 
                 121 
                 (All (Rp))-usucuAsGsAsccuGsuuuuGscuusdTsdT 
               
               
                 (4) 
                   
                   
               
               
                 PCSK9 
                 121 
                 (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, 
               
               
                 (5) 
                   
                 Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp)- 
               
               
                   
                   
                 ususcsusAsGsAscscsusGsususususGscsususdTsdT 
               
               
                 PCSK9 
                 121 
                 (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, 
               
               
                 (6) 
                   
                 Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp)- 
               
               
                   
                   
                 ususcsusAsGsAscscsusGsususususGscsususdTsdT 
               
               
                   
               
               
                 NOTE: 
               
               
                 lower case letters represent 2′-OMe RNA residues; 
               
               
                 capital letters represent RNA residues; 
               
               
                 d = 2′-deoxy residues; and 
               
               
                 “s” indicates a phosphorothioate moiety. 
               
            
           
         
       
     
     Synthesis examples for Human PCSK9 siRNA Antisense Strands having several chiral phosphorothioate internucleotide linkages and full chiral phosphorothioate internucleotide linkages. 
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                 SEQ 
                   
               
               
                   
                 ID  
                   
               
               
                   
                 NO: 
                 Human PCSK9 siRNA Antisense Strands 
               
               
                   
               
             
            
               
                 PCSK9 (7) 
                 122 
                 (All (Rp))- AsAsGscsAsAsAsAscsAsGsGsUsCsusAsGsAsAsdTsdT 
               
               
                 PCSK9 (8) 
                 122 
                 (All (Sp))- AsAsGscsAsAsAsAscsAsGsGsUsCsusAsGsAsAsdTsdT 
               
               
                 PCSK9 (9) 
                 122 
                 (All (Rp))- AsAGcAAAAcsAsGsGsUsCsusAsGsAsAsdTsdT 
               
               
                 PCSK9 (10) 
                 122 
                 (All (Sp))- AsAGcAAAAcsAsGsGsUsCsusAsGsAsAsdTsdT 
               
               
                 PCSK9 (11) 
                 122 
                 (All (Rp))- AAsGscsAsAsAsAscAGGUCuAGAAdTsdT 
               
               
                 PCSK9 (12) 
                 122 
                 (All (Sp))- AAsGscsAsAsAsAscAGGUCuAGAAdTsdT 
               
               
                 PCSK9 (13) 
                 122 
                 (All (Rp))- AsAsGscAsAsAsAscAsGsGsUsCsuAsGsAsAsdTsdT 
               
               
                 PCSK9 (14) 
                 122 
                 (All (Sp))- AsAsGscAsAsAsAscAsGsGsUsCsuAsGsAsAsdTsdT 
               
               
                 PCSK9 (15) 
                 122 
                 (All (Rp))- AsAGcAAAsAscAsGsGsUsCsusAsGsAsAsdTsdT 
               
               
                 PCSK9 (16) 
                 122 
                 (All (Sp))- AsAGcAAAsAscAsGsGsUsCsusAsGsAsAsdTsdT 
               
               
                 PCSK9 (17) 
                 122 
                 (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp)- 
               
               
                   
                   
                 AsAGcAAAsAscAsGsGsUsCsusAsGsAsAsdTsdT 
               
               
                 PCSK9 (18) 
                 122 
                 (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp)- 
               
               
                   
                   
                 AsAGcAAAsAscAsGsGsUsCsusAsGsAsAsdTsdT 
               
               
                   
               
               
                 NOTE: 
               
               
                 lower case letters represent 2′-OMe RNA residues; capital letters represent RNA residues; d = 2′-deoxy residues; and “s” indicates a phosphorothioate moiety. 
               
            
           
         
       
     
     Alternatively or additionally, the present invention provides for preparation of siRNA duplexes having several chiral phosphorothioate internucleotide linkages and full chiral phosphorothioate internucleotide linkages and fully modified ribose moieties. 
     For example, in certain embodiments, multiple chiral phosphorothioate linkages are introduced inside a fully ribose-modified RNA oligonucleotide by using the appropriate chiral RNA 3′-phosphoramidites, having the corresponding desired ribose 2′-chemical modification, such as 2′-deoxy-2′-fluoro (2′-F) or 2′-O-methyl (2′-OMe). To give but a few examples, each of the following Human PCSK9 siRNA fully modified 2′-F/2′-OMe Sense Strands having several chiral phosphorothioate internucleotide linkages and full chiral phosphorothioate internucleotide linkages, can be prepared. 
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                 SEQ 
                   
               
               
                   
                 ID 
                   
               
               
                   
                 NO: 
                 Human PCSK9 siRNA fully modified 2′-F/2′-OMe Sense Strands 
               
               
                   
               
             
            
               
                 PCSK9 (19) 
                 121 
                 (All (Rp))- UfsusCfsusAfsgsAfscsCfsusGfsusUfsusUfsgsCfsusUfsdTsdT 
               
               
                 PCSK9 (20) 
                 121 
                 (All (Sp))- UfsusCfsusAfsgsAfscsCfsusGfsusUfsusUfsgsCfsusUfsdTsdT 
               
               
                 PCSK9 (21) 
                 121 
                 (All (Rp))- UfsuCfsuAfsgAfscCfsuGfsuUfsuUfsgCfsuUfsdTsdT 
               
               
                 PCSK9 (22) 
                 121 
                 (All (Sp))- UfsuCfsuAfsgAfscCfsuGfsuUfsuUfsgCfsuUfsdTsdT 
               
               
                 PCSK9 (23) 
                 121 
                 (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, 
               
               
                   
                   
                 Sp)- UfsusCfsusAfsgsAfscsCfsusGfsusUfsusUfsgsCfsusUfsdTsdT 
               
               
                 PCSK9 (24) 
                 121 
                 (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, 
               
               
                   
                   
                 Rp)- UfsusCfsusAfsgsAfscsCfsusGfsusUfsusUfsgsCfsusUfsdTsdT 
               
               
                   
               
               
                 NOTE: 
               
               
                 lower case letters represent 2′-OMe RNA residues; capital letters represent 2′-F RNA residues; d = 2′-deoxy residues; and “s” indicates a phosphorothioate moiety. 
               
            
           
         
       
     
     Synthesis examples for Human PCSK9 siRNA fully modified 2′-F/2′-OMe Antisense Strands having several chiral phosphorothioate internucleotide linkages and full chiral phosphorothioate internucleotide linkages. 
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                 SEQ 
                   
               
               
                   
                 ID 
                   
               
               
                   
                 NO: 
                 Human PCSK9 siRNA fully modified 2′-F/2′-OMe Antisense Strands 
               
               
                   
               
             
            
               
                 PCSK9 (25) 
                 122 
                 (All (Rp))- asAfsgsCfsasAfsasAfscsAfsgsGfsusCfsusAfsgsAfsasdTsdT 
               
               
                 PCSK9 (26) 
                 122 
                 (All (Sp))- asAfsgsCfsasAfsasAfscsAfsgsGfsusCfsusAfsgsAfsasdTsdT 
               
               
                 PCSK9 (27) 
                 122 
                 (All (Rp))- asAfgCfaAfaAfcsAfsgsGfsusCfsusAfsgsAfsasdTsdT 
               
               
                 PCSK9 (28) 
                 122 
                 (All (Sp))- asAfgCfaAfaAfcsAfsgsGfsusCfsusAfsgsAfsasdTsdT 
               
               
                 PCSK9 (29) 
                 122 
                 (All (Rp))- asAfsgCfsaAfsaAfscAfsgGfsuCfsuAfsgAfsadTsdT 
               
               
                 PCSK9 (30) 
                 122 
                 (All (Sp))- asAfsgCfsaAfsaAfscAfsgGfsuCfsuAfsgAfsadTsdT 
               
               
                 PCSK9 (31) 
                 122 
                 (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp)- 
               
               
                   
                   
                 asAfgCfaAfasAfscAfsgsGfsusCfsusAfsgsAfsasdTsdT 
               
               
                 PCSK9 (32) 
                 122 
                 (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp)- 
               
               
                   
                   
                 asAfgCfaAfasAfscAfsgsGfsusCfsusAfsgsAfsasdTsdT 
               
               
                   
               
               
                 NOTE: 
               
               
                 lower case letters represent 2′-OMe RNA residues; capital letters represent 2′-F RNA residues; d = 2′-deoxy residues; and “s” indicates a phosphorothioate moiety. 
               
            
           
         
       
     
     To assemble duplexes, RNA strand thermal annealing and preparation of siRNA duplexes is performed. Specifically, each RNA strand is mixed with its complementary RNA strand in equimolar concentration of 10 μM in 1×PBS. Total 0.5 mL solution is prepared for each duplex and the mixture is heated at 90° C. for 2 min and is allowed to cool down over the course of several hours. The mixtures are then stored at 4° C. 
     Following the thermal RNA strand annealing step, all the possible siRNA duplex combinations can be prepared by annealing any of the Sense strands with any possible complementary strand of the Antisense strands. 
     All prepared siRNA duplexes can be evaluated in vitro for their PCSK9 gene-silencing properties, following transfection in HeLa cells or Hep3B cells (e.g., as described herein). According to the present invention, different potencies may be observed for different duplexes, e.g., that vary in number, position, and/or stereo architecture of the chiral phosphorothioate backbone linkages, and optionally also in presence, level, and or type of one or more other chemical modifications. 
     Any or all siRNA properties such as: nuclease resistance, cell penetration, endosomal escape, duplex thermodynamic stability, tridemnsional structure of the duplex, affinity towards the various mechanistic steps of enzyme interactions, affinity towards the target mRNA, specific off-target effects, immunostimulation, duration of action, pharmacokinetics, etc. may be modulated and influenced by the stereochemistry of the chiral phosphorothioate backbone linkages, as described herein. 
     Example 64. Preparation of Chirally Controlled Preparations of Oligonucleotide 
     
       
         
         
             
             
         
       
     
     The present Example describes preparation of a variety of particular chirally controlled compositions of certain oligonucleotides described therein. 
     N 4 -Acetyl-5′-O-DMTr-2′-O-methylcytidine (1) (1.15 g, 1.91 mmol) was dissolved in anhydrous DCM (20 mL) and mixed with 2 equiv of succinic anhydride (0.383 g, 3.82 mmol) and 3 equiv of 4-N,N-dimethylaminopyridine (0.701 g, 5.73 mmol). The reaction was stirred under Argon at room temperature. After complete consumption of starting material as determined by TLC (1 hour), the solvents were evaporated to dryness, the crude residue was dissolved in DCM containing 1% triethylamine then purified by flash silica gel chromatography using a gradient of 0-2% of MeOH in DCM containing 2% of triethylamine. Yield of pure succinyl compound (2) after evaporation was 1.50 g, 98%. MS (ESI +ve): calc (M+H) + : 702.27, found: 702.34. The resulting N 4 -acetyl-5′-O-DMTr-3′-O-succinyl-2′-O-methylcytidine (2) (0.18 g, 0.22 mmol), N,N-diisopropylethylamine (0.18 mL, 0.79 mmol) and GE Custom Support™ Amino (1 g) were taken up in DMF (5 mL) then HBTU (0.10 g, 0.26 mmol) was added. The mixture was shaken for 2 h then filtered. The support was washed with DMF, MeOH and finally DCM then dried in vacuo. Trityl cation analysis (monitoring at 504 nm) showed that the loading of nucleoside on the support (3) was 180 μmol/g. 
     Example 65. Preparation of Chirally Controlled Preparations of Oligonucleotide 
     
       
         
         
             
             
         
       
     
     Using a procedure analogous to that described in Example 64, N 2 -Phenoxyacetyl-5′-O-DMTr-3′-O-succinyl-2′-O-methylguanosine (4, MS (ESI +ve): calc (M+H) + : 834.30, found: 834.32) was loaded onto GE Custom Support™ Amino. Trityl cation analysis (monitoring at 504 nm) showed that the loading of nucleoside on the support (6) was 140 μmol/g. 
     In some embodiments, oligonucleotides containing stereodefined 2′-OMe phosphorothioate triester internucleotidic linkages was synthesized on ABI 394 DNA/RNA synthesizer according to the cycle summarized in Table E-27 using a 10 μmol capacity synthesis column loaded with 60 mg (10.8 and 8.4 μmol respectively) of either uncapped succinyl linked 5′-O-DMTr-2′-O-methyl-G Pac  (6, 140 μmol/g) or 5′-O-DMTr-2′-O-methyl-C Ac  (3, 180 μmol/g). The synthesis cycle was performed with a preliminary capping step (capping 2) and with removal of the terminal 5′-O-DMTr group. The stereospecific sulfurization steps were performed using the 0.3 M S-(2-cyanoethyl)methylthiosulfonate reagent in ACN containing BSTFA, following the coupling of the corresponding chiral phosphoramide and the two-step capping process (Table E-27). 
     Once the automated oligonucleotide synthesis cycle was complete, and the final 5′-O-DMTr group removed, the synthesis column was taken off the DNA/RNA synthesizer and dried under vacuum. The dried support was transferred onto an empty glass manual peptide synthesizer and 10 mL solution of 0.5 M 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 0.25 M N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) in ACN was continuously added to the support for 5 min without stopping the flow in the manual peptide synthesizer. The support was washed by ACN and dried in vacuo. Then, the support was treated with 5% EtOH/conc NH 3  (5 mL) at 60° C. for 6 h, and left at room temperature for 12 h. The support was removed by filtration and washed with conc. NH 3 . The filtrate was concentrated in vacuo then purified by IEX. 
     
       
         
           
               
             
               
                 TABLE E-27 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis. 
               
            
           
           
               
               
               
               
               
            
               
                 step 
                 reaction 
                 reagent 
                 delivery time (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 120 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M 
                 8 + 6 
                 30 + 900 
               
               
                   
                   
                 phosphoramidite in 
                   
                   
               
               
                   
                   
                 ACN + 2M CMPT in 
                   
                   
               
               
                   
                   
                 ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6- 
                 30 
                 60 
               
               
                   
                   
                 lutidine 
                   
                   
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6- 
                 30 
                 60 
               
               
                   
                   
                 lutidine + 16% NMI in 
                   
                   
               
               
                   
                   
                 THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M (S-cyanoethyl)- 
                 15 + 3 × 4 
                 120 + 3 × 60 + 300 
               
               
                   
                   
                 methylthiosulfonate 
                   
                   
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Synthesis of Oligonucleotide ONT-94: (All (Sp))-gsgsusgsgsasasgsgsc (SEQ ID NO: 124). t R  (IEX-HPLC): 18.26 min. Calc MW: 3563.9; Found MW: 3562.6. 
     Synthesis of Oligonucleotide ONT-96: (All (Rp))-gsgsusgsgsasasgsgsc (SEQ ID NO: 124). t R  (IEX-HPLC): 18.16 min. Calc MW: 3563.9; Found MW: 3561.7. 
     Synthesis of Oligonucleotide ONT-98: (Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp)-gsgsusgsgsasasgsgsc (SEQ ID NO: 124). t R  (IEX-HPLC): 18.05 min. Calc MW: 3563.9; Found MW: 3562.5. 
     Synthesis of Oligonucleotide ONT-100: (Sp, Rp, Sp, Rp, Sp, Rp, Sp, Rp, Sp)-gsgsusgsgsasasgsgsc (SEQ ID NO: 124). t R  (IEX-HPLC): 17.86 min. Calc MW: 3563.9; Found MW: 3561.1. 
     Synthesis of Oligonucleotide ONT-102: (Rp, Rp, Sp, Sp, Sp, Sp, Sp, Rp, Rp)-gsgsusgsgsasasgsgsc (SEQ ID NO: 124). t R  (IEX-HPLC): 18.30 min. Calc MW: 3563.9; Found MW: 3561.3. 
     Synthesis of Oligonucleotide ONT-104: (Sp, Sp, Rp, Rp, Rp, Rp, Rp, Sp, Sp)-gsgsusgsgsasasgsgsc (SEQ ID NO: 124). t R  (IEX-HPLC): 17.95 min. Calc MW3563.9; Found MW: 3562.7. 
     Synthesis of Oligonucleotide ONT-95: (All (Sp))-gscscsuscscsasg. t R  (IEX-HPLC): 14.78 min. Calc MW: 2709.2; Found MW: 2707.4. 
     Synthesis of Oligonucleotide ONT-97: (All (Rp))-gscscsuscscsasg. t R  (IEX-HPLC): 15.60 min. Calc MW: 2709.2; Found MW: 2708.3. 
     Synthesis of Oligonucleotide ONT-99: (Rp, Sp, Rp, Sp, Rp, Sp, Rp)-gscscsuscscsasg. t R  (IEX-HPLC): 16.10 min. Calc MW: 2709.2; Found MW: 2708.0. 
     Synthesis of Oligonucleotide ONT-101: (Sp, Rp, Sp, Rp, Sp, Rp, Sp)-gscscsuscscsasg. t R  (IEX-HPLC): 16.23 min. Calc MW: 2709.2; Found MW: 2708.2. 
     Synthesis of Oligonucleotide ONT-103: (Rp, Rp, Sp, Sp, Sp, Rp, Rp)-gscscsuscscsasg. t R  (IEX-HPLC): 16.26 min. Calc MW: 2709.2; Found MW: 2707.8. 
     Synthesis of Oligonucleotide ONT-105: (Sp, Sp, Rp, Rp, Rp, Sp, Sp)-gscscsuscscsasg. t R  (IEX-HPLC): 16.22 min. Calc MW: 2709.2; Found MW: 2710.0. 
     Oligonucleotides containing stereodefined chimeric 2′-OMe phosphorothioate triester and 2′-O-MOE phosphorothioate triester internucleotidic linkages was synthesized on ABI 394 DNA/RNA in an analogous fashion to those described in examples herein. 
     Synthesis of Oligonucleotide ONT-90: (All (Rp))-G MOE sG MOE susG MOE sG MOE sasasG MOE sG MOE sc (SEQ ID NO: 124). t R  (IEX-HPLC): 15.35 min. Calc MW: 3828.2; Found MW: 3826.5. 
     Synthesis of Oligonucleotide ONT-119: (All (Sp))-G MOE sG MOE susG MOE sG MOE sasasG MOE sG MOE sc (SEQ ID NO: 124). t R  (IEX-HPLC): 16.42 min. Calc MW: 3828.2; Found MW: 3827.2. 
     Synthesis of Oligonucleotide ONT-91: (All (Rp))-G MOE scscsuscscsasg. t R  (IEX-HPLC): 15.69 min. Calc MW: 2753.3; Found MW: 2751.5. 
     Synthesis of Oligonucleotide ONT-120: (All (Sp))-G MOE scscsuscscsasg. t R  (IEX-HPLC): 14.71 min. Calc MW: 2753.3; Found MW: 2751.4. 
     Example 66. General IEX-HPLC Method for the Analysis of Crude and Purified DMT Off RIPtide Oligonucleotides 
     Buffer A: 10 mM TrisHCl, 50% ACN, pH 8.0 
     Buffer B: 10 mM TrisHCl, 800 mM NaClO 4 , 50% ACN, pH 8.0 
     Column: DIONEX, DNAPac, PA-200, Analytical, 4.0×250 mm 
     Column temperature=60° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 95 
                 5 
                   
               
               
                 2 
                 1 
                 95 
                 5 
                 1 
               
               
                 22 
                 1 
                 70 
                 30 
                 6 
               
               
                 25 
                 1 
                 5 
                 95 
                 6 
               
               
                 25.5 
                 1 
                 95 
                 5 
                 6 
               
               
                 30 
                 1 
                 95 
                 5 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 67. General UPLC-LCMS Method for the Analysis of Purified DMT Off RIPtide Oligonucleotides 
     Buffer A: 15 mM TEA, 400 mM HFIP, Water 
     Buffer B: 50:50 Buffer A/Methanol 
     Column: UPLC@OST C 18  1.7 μm, 2.1×500 mm 
     Column temperature=50° C. 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                 0.2 
                 80 
                 20 
                   
               
               
                 2 
                 0.2 
                 80 
                 20 
                 1 
               
               
                 22 
                 0.2 
                 30 
                 70 
                 6 
               
               
                 25 
                 0.2 
                 5 
                 95 
                 6 
               
               
                 25.5 
                 0.2 
                 80 
                 20 
                 6 
               
               
                 30 
                 0.2 
                 80 
                 20 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 68. General IEX-HPLC Method for the Purification of Crude DMT Off RIPtide Oligonucleotide 
     Buffer A: 20 mM NaOH, pH 11.0 
     Buffer B: 20 mM NaOH, 2.5 M NaCl, pH 11.0 
     Column: Empty column Waters AP-1 (Waters), custom in-house packed with Source 15Q support (GE Healthcare). The same purification column was used for the different stereopure RIPtide oligonucleotides. 
     Instrument: AKTA Purifier, equipped with the P-900 pump, the UPC-900 detector and a 50 mL injection SuperLoop (GE Healthcare) 
     Buffer heater temperature set=70° C. 
     Column heater tape set=70° C. 
     Signal monitored at 254 nm 
     Fractions volume: 5 mL 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 100 
                 0 
                   
               
               
                 15 
                 4 
                 100 
                 0 
                 1 
               
               
                 25 
                 4 
                 90 
                 10 
                 6 
               
               
                 35 
                 4 
                 90 
                 10 
                 1 
               
               
                 45 
                 4 
                 80 
                 20 
                 6 
               
               
                 60 
                 4 
                 80 
                 20 
                 1 
               
               
                 80 
                 4 
                 65 
                 35 
                 6 
               
               
                 95 
                 4 
                 65 
                 35 
                 1 
               
               
                 120 
                 4 
                 45 
                 55 
                 6 
               
               
                 125 
                 4 
                 45 
                 55 
                 1 
               
               
                 126 
                 4 
                 100 
                 0 
                 6 
               
               
                 140 
                 4 
                 100 
                 0 
                 1 
               
               
                   
               
            
           
         
       
     
     Collected fractions were individually analyzed by analytical IEX-HPLC using the analytical conditions and gradient described above. Pure fractions were pooled in order to provide purified material of 95% and above purity as determined by the 254 nm UV absorbance profiles. 
     General RP-Sep-Pak method for the desalting of purified RIPtides oligonucleotides. The solution of pooled pure fractions was loaded on a Sep-Pak cartridge (Waters, Sep-Pak Vac 35 cc (10 g) C 18  Cartridges) pre-conditioned with water. After loading of the sample (100 mL), the cartridge was washed with milli Q water (50 mL) to remove all salt and then washed with 50% ACN/water to elute the full length desalted RNA oligonucleotide. The collected solution was concentrated in vacuo to a volume of 5 mL and lyophilized from water. 
     A panel of stereocontrolled fully phosphorothioate modified RIPtides (8-mer or 10-mer) that bind naked hTR are investigated for the in vitro inhibition of the activity of the telomerase RNP complex. The Cy5-TRAP assay (Shay et al.,  Nat. Protoc.,  2006, 1, 1583), a variation of the TRAP (Shay et al.,  Science,  1994, 266, 2011), is used to the determine the IC 50  values for the stereocontrolled phosphorothioate RIPtides using HeLa cell extracts, according to previously reported protocols (Verdine et al.,  J. Biol. Chem.,  2012, 287, 18843). 
     TRAP assays are performed following previously reported protocols that use fluorescence as a quantitation system, with some modifications. Briefly, extension of a fluorescent artificial substrate by telomerase is carried out for 30 minutes at 30° C., followed by amplification with 30 PCR cycles (34° C. 30 s, 59° C. 30 s, 72° C. 1 min). The inhibitory potential of the RIPtides is initially assessed in HeLa cell extracts, in duplicate experiments, using a 600 μM-60 μM concentration range. Experiments with selected RIPtides are repeated for a concentration range of 0.06 μM-60 μM in HeLa, DU145 (prostate cancer) and HEK293 cell extracts. Several controls are used in these assays: a positive control (untreated cell lysate), negative controls (buffer only, heat inactivated and RNase treated cell extracts), and PCR amplification control (60 μM of RIPtide added after telomerase elongation and before PCR). In certain embodiments, chirally controlled RIPtide preparations show enhanced inhibition of hTR as compared with the positive control and/or enhanced inhibition of hTRas compared with the negative control; in some embodiments, some, but optionally not all RIPtides of a given sequence show such properties. In some embodiments, by “enhanced” in this context is meant enhanced by between about five-fold and about ten-fold. In some embodiments, by “enhanced” is meant at least about 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, or 10.0-fold. 
     Cell Culturing Conditions. The transformed embryonic kidney cell line HEK293 and the prostate cancer cell line DU145 are maintained in DMEM supplemented with 10% fetal bovine serum in 5% CO 2  at 37° C. Soluble cell extracts for TRAP assays are prepared by detergent lysis of 106 cells with 200 μL 1×CHAPS Lysis Buffer (Chemicon) as described in the manufacturer&#39;s instructions. 
     According to the present invention, different potencies and/or different other properties may be observed for the various stereoarchitectures of fully stereocontrolled phosphorothioate RIPtides. For instance, RIPtide properties such as: nuclease resistance, tissue accumulation, cell penetration, endosomal escape, tridemnsional structure of the RIPtide, affinity towards the target folded hTR RNA, immunostimulation, duration of action, pharmacokinetics, etc. may vary for chirally controlled RIPtide preparations that share the same sequence but differ from one another with respect to different location and/or stereochemistry of one or more chiral phosphorothioate backbone linkages. 
     Example 69: Chirally Controlled Oligonucleotide Compositions Show Different Activity In Vivo as Compared with Chirally Uncontrolled Compositions Having the Same Sequence 
     The present Example compares in vivo pharmacological activity of chirally pure oligonucleotides with that observed for the “parent” stereorandom mixture (i.e., for a composition containing oligonucleotides of the same sequence as the chirally pure oligonucleotides but not displaying chiral purity, for example as a result of having been prepared via a stereorandom process). Four chirally pure oligonucleotides, each of which had a sequence complementary to that of a particular target transcript or gene encoding a protein of interest were synthesized, formulated, and administered twice per week for 5 weeks at two dose levels each, to animals expressing the target gene. Levels of encoded protein levels were quantified. In this Example, oligonucleotides having a sequence antisense to (and therefore targeting) human Apolipoprotein-B (ApoB) were used for proof-of-concept in transgenic mice expressing human ApoB. 
     Test Articles 
     Test articles were PBS alone (i.e., no oligonucleotide control) or the relevant oligonucleotide composition (i.e., Mipomersen (ONT-41), ONT-75, ONT-77, ONT-80, or ONT-81 oligonucleotides (see Example 52 for structures) were formulated in 1×PBS (diluted from 10×PBS (Life Technologies, AM9624) using nuclease-free water (Qiagen, 129115)) at 0.5 and 1 mg/mL for dosing at 5 and 10 mg/kg, respectively. Formulations were based on absolute mass with no adjustment for the active material. Oligonucleotide concentrations were confirmed by measurement with a Carry-100 UV-Vis (Agilent Technologies). Samples of all test articles were checked for endotoxin levels using a kinetic pyrochrome chromogenic endotoxin assay (Associates of Cape Cod, 1500-5, E005-5). All tested samples were found to have a lower amount of endotoxin than the 0.5 EU/ml acceptable limit. 
     Animals and In Vivo Procedures 
     Female transgenic mice (huApoB mice) expressing high plasma concentrations of human apolipoprotein B100 and lipoprotein(a) (J. Clin. Invest. 92: 3029-3037) were obtained from Taconic (strain B6.SJL-Tg(APOB)|102Sgy N20+?, model #1004-F). All animals were genotyped prior to delivery. 
     Mice were delivered and allowed to acclimate for at least seven days prior to study start. All mice were given regular chow and water ad libitum, and were not fasted prior to compound administration. Mice were randomized to study groups, and dosed intraperitoneally (IP) at 10 ml/kg on Days 1, 4, 8, 11, 15, 18, 22, 25, 29 and 32, based on individual mouse body weight measured prior to dosing on each dosing day. Blood was collected during the course of the study on Days 0 (day before first dose), 17, 24, 31, 38, 45, 52 and 60 by submandibular (cheek) bleed and at sacrifice on Day 66 by cardiac puncture, and then processed to serum. 
     Apolipoprotein B Protein Assay 
     ApoB protein levels in serum were measured using an ApoB Human ELISA Kit (Abcam, ab108807). Serum samples were diluted 1:20,000 and assayed per the kit recommended protocol without modifications. Automated washes were done with 30 second soak periods using an Aquamax 4000 (Molecular Devices). Reactions were stopped after 12 minute incubations with Chromogen substrate and measurements were taken with a Spectramax M5 (Molecular Devices). 
     Standard curves were generated by plotting the standard concentration on the x-axis and the corresponding mean 450 nm absorbance on the y-axis. The best-fit line was determined by regression analysis using log-log curve-fit. Each assay plate included a negative (PBS treated) and a positive (Mipomersen-treated) control and each mouse serum sample was assayed in 4 technical replicates. 
     For each sample, the mean absolute level of ApoB was normalized to the mean value for the PBS treated group, to obtain the relative level of ApoB protein expression. In one instance, an animal was excluded from the analysis as measurements deviated from the cohort mean by 2 standard deviations. Relative levels of ApoB protein expression were used for statistical analysis (Graphpad Prism) by 2-way ANOVA followed by Newman-Keuls post-hoc test. 
     Results 
     The results are shown in Table E-28a and  FIG.  40   . Relative to PBS, 5 mg/kg IP ONT-75 resulted in significant reductions of serum ApoB protein levels on day 24 of the study, to 87% (p&lt;0.05). Relative to PBS, 5 mg/kg IP ONT-77 resulted in significant reductions of serum ApoB protein levels on days 24, and 31 of the study, to 72% (p&lt;0.0001), 81% (p&lt;0.05) of control levels, respectively. Relative to PBS, 5 mg/kg IP ONT-80 resulted in significant reductions of serum ApoB protein levels on days 24 and 31 of the study, to 83% (p&lt;0.05), 80% (p&lt;0.05) of control levels, respectively. Relative to PBS, 5 mg/kg IP ONT-81 resulted in significant reductions of serum ApoB protein levels on days 17 and 24 of the study, to 85% (p&lt;0.05), 70% (p&lt;0.0001) of control levels, respectively. 
     
       
         
           
               
             
               
                 TABLE E-28a 
               
             
            
               
                   
               
               
                 Serum Human Apolipoprotein B Levels Relative to PBS 
               
               
                 After Multiple IP Doses of 5 mg/kg Stereioisomer or 
               
               
                 Mipomersen in huApoB Mice (N = 4-5] 
               
            
           
           
               
               
            
               
                   
                 Day 
               
            
           
           
               
               
               
               
               
               
            
               
                 Sample 
                 17 
                 24 
                 31 
                 38 
                 45 
               
               
                   
               
               
                 PBS 
                 100 ± 9     
                 100 ± 9 
                 100 ± 9      
                 100 ± 11     
                 100 ± 9     
               
               
                 ONT-41 
                     54 ± 11 
                  49 ± 7 
                 54 ± 9     
                     64 ± 10 
                  80 ± 9 
               
               
                 ONT-75 
                 103 d  ± 11 
                 87 dw  ± 10 
                 94 d  ± 10 
                 97 d  ± 10 
                 98 a  ± 9 
               
               
                 ONT-77 
                  83 d  ± 15 
                  72 cz  ± 10 
                 81 dw  ± 9  
                 91 d  ± 13 
                  95 a  ± 12 
               
               
                 ONT-80 
                 89 d  ± 9 
                 83 dw  ± 8  
                 80 dw  ± 3  
                 95 d  ± 7  
                     87 ± 11 
               
               
                 ONT-81 
                  85 dw ± 12 
                 70 cz  ± 5  
                 88 d  ± 15 
                 101 d  ± 10  
                 102 b  ± 7  
               
               
                   
               
               
                   a Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.05 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   b Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.01 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   c Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   d Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.0001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   w Statistically different from the PBS control group, with p &lt; 0.5 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                 x: Statistically different from the PBS control group, with p &lt; 0.01 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                 y: Statistically different from the PBS control group, with p &lt; 0.001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   z Statistically different from the PBS control group, with p &lt; 0.0001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
            
           
         
       
     
     Compared with 5 mg/kg IP Mipomersen, ONT-75 administered with the same dosing paradigm resulted in significantly less reduction of serum ApoB protein levels on days 17, 24, 31 and 38 (p&lt;0.0001), and 45 (p&lt;0.05) of the study. Compared with 5 mg/kg IP Mipomersen, ONT-77 administered with the same dosing paradigm resulted in significantly less reduction of serum ApoB protein levels on days 17, 31 and 38 (p&lt;0.0001), 24 (p&lt;0.001) and 45 (p&lt;0.05) of the study. Compared with 5 mg/kg IP Mipomersen, ONT-80 administered with the same dosing paradigm resulted in significantly less reduction of serum ApoB protein levels on days 17, 24, 31 and 38 (p&lt;0.0001), but was not different on day 45 (p&gt;0.05) of the study. Compared with 5 mg/kg IP Mipomersen, ONT-81 administered with the same dosing paradigm resulted in significantly less reduction of serum ApoB protein levels on days 17, 31 and 38 (p&lt;0.0001), 24 (p&lt;0.001) and 45 (p&lt;0.01) of the study.  FIG.  40    shows the timecourse of Serum Human Apolipoprotein B protein levels relative to PBS after 5 mg/kg stereoisomer or mipomersen IP dosing in huApoB mice. Downward arrows indicate dosing days. Group means normalized to the PBS control group are shown, where each groups comprised 4-5 animals. Error bars represent standard deviations. 
     The results of the 10 mg/kg dosing paradigm are shown in Table E-28b and  FIG.  53   . Relative to PBS, 10 mg/kg IP ONT-75 resulted in significant reductions of serum ApoB protein levels on days 17, 24 and 38 of the study, to 78% (p&lt;0.0001), 76% (p&lt;0.0001) and 82% (p&lt;0.001), respectively. Relative to PBS, 10 mg/kg IP ONT-77 resulted in significant reductions of serum ApoB protein levels on days 17, 24, 31, 38, 45 and 52 of the study, to 62% (p&lt;0.0001), 61% (p&lt;0.0001), 54% (p&lt;0.0001), 59% (p&lt;0.0001), 72% (p&lt;0.0001), and 73% (p&lt;0.0001) of control levels, respectively. Relative to PBS, 10 mg/kg IP ONT-80 resulted in significant reductions of serum ApoB protein levels on days 17, 24, 31, 38, 45, 52 and 66 of the study, to 55% (p&lt;0.0001), 59% (p&lt;0.0001), 73% (p&lt;0.0001), 67% (p&lt;0.0001), 76% (p&lt;0.0001), 76% (p&lt;0.0001), and 80% (p&lt;0.01) of control levels, respectively. Relative to PBS, 10 mg/kg IP ONT-81 resulted in significant reductions of serum ApoB protein levels on days 17, 24, 31, 38 and 45 of the study, to 49% (p&lt;0.0001), 62% (p&lt;0.0001), 71% (p&lt;0.0001), 74% (p&lt;0.0001) and 79% (p&lt;0.001) of control levels, respectively. 
     
       
         
           
               
             
               
                 TABLE E-28b 
               
             
            
               
                   
               
               
                 Serum Human Apolipoprotein B Levels Relative to PBS After Multiple IP Doses of 10 mg/kg 
               
               
                 Stereioisomer (ONT-75, -77, -80 or -81) or Mipomersen in huApoB Mice (N = 4-5) 
               
            
           
           
               
               
            
               
                   
                 Day 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 Sample 
                 17 
                 24 
                 31 
                 38 
                 45 
                 52 
                 60 
                 66 
               
               
                   
               
               
                 PBS 
                     100 ± 11 
                  100 ± 9 
                     100 ± 17 
                  100 ± 11 
                 100 ± 7  
                 100 ± 7     
                 100 ± 9  
                 100 ± 7     
               
               
                 ONT-41 
                     31 ± 13 
                     24 ± 24 
                     23 ± 6 
                     29 ± 10 
                     46 ± 10 
                  64 ± 8 
                 77 ± 8 
                  64 ± 7 
               
               
                 ONT-75 
                 78 dz  ± 5 
                 76 dz  ± 4 
                 82 dy  ± 7 
                 95 d  ± 9     
                 97 d  ± 3     
                  94 d  ± 13 
                 108 d  ± 6      
                  87 c  ± 12 
               
               
                 ONT-77 
                 62 dz  ± 9 
                 61 dz  ± 4 
                 54 dz  ± 6 
                 59 dz  ± 10 
                 72 dz  ± 7  
                 73 z  ± 7 
                 95 a  ± 5  
                  87 b  ± 11 
               
               
                 ONT-80 
                 55 cz  ± 4 
                 59 dz  ± 3 
                 73 dz  ± 4 
                 67 dz  ± 10 
                 76 dz  ± 10 
                  76 z  ± 11 
                  87 ± 10 
                 80 bx  ± 7  
               
               
                 ONT-81 
                 49 bz  ± 7 
                  62 dz  ± 19 
                 71 dz  ± 7 
                 74 dz  ± 7  
                 79 dy  ± 11 
                 88 c  ± 5 
                 90 ± 6 
                 89 c  ± 8 
               
               
                   
               
               
                   a Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.05 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   b Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.01 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   c Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   d Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.0001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                 w: Statistically different from the PBS control group, with p &lt; 0.05 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   x Statistically different from the PBS control group, with p &lt; 0.01 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   y Statistically different from the PBS control group, with p &lt; 0.001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   z Statistically different from the PBS control group, with p &lt; 0.0001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
            
           
         
       
     
     Compared with 10 mg/kg IP Mipomersen, ONT-75 administered with the same dosing paradigm resulted in significantly lower reduction (knockdown) of serum ApoB protein levels on days 17, 24, 31, 38, 45, 52, 60 (p&lt;0.0001), and 66 (p&lt;0.001) of the study. Compared with 10 mg/kg IP Mipomersen, ONT-77 administered with the same dosing paradigm resulted in significantly lower reduction (knockdown) of serum ApoB protein levels on days 17, 24, 31, 38, and 45 (p&lt;0.0001), 60 (p&lt;0.05) and 66 (p&lt;0.01) of the study. Compared with 10 mg/kg IP Mipomersen, ONT-80 administered with the same dosing paradigm resulted in significantly lower reduction (knockdown) of serum ApoB protein levels on days 24, 31, 38 and 45 (p&lt;0.0001), 17 (p&lt;0.001) and 66 (p&lt;0.01). Compared with 10 mg/kg IP Mipomersen, ONT-81 administered with the same dosing paradigm resulted in significantly lower reduction (knockdown) of serum ApoB protein levels on days 24, 31, 38 and 45 (p&lt;0.0001), 17 (p&lt;0.01), 52 and 66 (p&lt;0.001) of the study. 
     By day 38 (i.e. 6 days after the last dose), serum ApoB protein levels had returned to baseline after ONT-75. Considering the initial serum ApoB protein level reduction, the rate of return to baseline of serum ApoB protein was slower after ONT-77 and ONT-80 and the level of serum ApoB protein is similar to Mipomersen levels by day 52. 
     The results of the 5 mg/kg dosing paradigm of additional stereopure architectures are shown in Table E-28c and  FIG.  54   . Relative to PBS, 5 mg/kg IP ONT-87 resulted in significant reductions of serum ApoB protein levels on days 17, 24, 31 and 38 of the study, to 27% (p&lt;0.0001), 40% (p&lt;0.0001), 55% (p&lt;0.0001) and 71% (p&lt;0.0001) of control levels, respectively. Relative to PBS, 5 mg/kg IP ONT-88 resulted in significant reductions of serum ApoB protein levels on days 17, 24, 31 and 38 of the study, to 47% (p&lt;0.0001), 34% (p&lt;0.0001), 69% (p&lt;0.0001) and 85% (p&lt;0.0001) of control levels, respectively. Relative to PBS, 5 mg/kg IP ONT-89 resulted in significant reductions of serum ApoB protein levels on days 17, 24 and 31 of the study, to 43% (p&lt;0.0001), 48% (p&lt;0.0001) and 85% (p&lt;0.05) of control levels, respectively. 
     
       
         
           
               
             
               
                 TABLE E-28c 
               
             
            
               
                   
               
               
                 Serum Human Apolipoprotein B Levels Relative to PBS After 
               
               
                 Multiple IP Doses of 5 mg/kg Stereioisomer (ONT-87, 
               
               
                 -88 or -89) or Mipomersen in huApoB Mice (N = 3-4) 
               
            
           
           
               
               
               
            
               
                   
                 Day 
                   
               
            
           
           
               
               
               
               
               
            
               
                 SAMPLES 
                 17 
                 24 
                 31 
                 38 
               
               
                   
               
               
                 PBS 
                 100 ± 5     
                  100 ± 12 
                 100 ± 4  
                 100 ± 8     
               
               
                 ONT-41 
                 33 z  ± 3 
                 44 z  ± 2 
                     75 y  ± 11 
                 76 y  ± 8 
               
               
                 ONT-87 
                  27 z  ± 11 
                 40 z  ± 5 
                 55 bz  ± 8      
                 71 z  ± 7 
               
               
                 ONT-88 
                 47 az  ± 5  
                 34 z  ± 8 
                  69 z  ± 13 
                 85 z  ± 7 
               
               
                 ONT-89 
                 43 z  ± 9 
                 48 z  ± 6 
                 85 w  ± 12 
                 93 a  ± 7 
               
               
                   
               
               
                   a Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.05 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   b Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.01 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                 c: Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                 d: Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.0001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   w Statistically different from the PBS control group, with p &lt; 0.05 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                 x: Statistically different from the PBS control group, with p &lt; 0.01 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   y Statistically different from the PBS control group, with p &lt; 0.001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   z Statistically different from the PBS control group, with p &lt; 0.0001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
            
           
         
       
     
     Compared with 5 mg/kg IP Mipomersen, ONT-87 administered with the same dosing paradigm resulted in significantly higher reduction (knock-down) of serum ApoB protein levels on day 31, (p&lt;0.01) of the study. Compared with 5 mg/kg IP Mipomersen, ONT-88 administered with the same dosing paradigm resulted in significantly lower reduction (knock-down) of serum ApoB protein levels on day 17 (p&lt;0.05 of the study). Compared with 5 mg/kg IP Mipomersen, ONT-89 administered with the same dosing paradigm resulted in significantly lower reduction (knockdown) of serum ApoB protein levels on day 38 (p&lt;0.05) of the study. 
     The results of the 10 mg/kg dosing paradigm of additional stereopure architectures are shown in Table E-28d and  FIGS.  55  and  56   . Relative to PBS, 10 mg/kg IP ONT-87 resulted in significant reductions of serum ApoB protein levels on days 17, and 24 of the study, to 27% (p&lt;0.0001), 14% (p&lt;0.0001) of control levels, respectively. Relative to PBS, 10 mg/kg IP ONT-88 resulted in significant reductions of serum ApoB protein levels on days 17, and 24 of the study, to 23% (p&lt;0.0001), and 17% (p&lt;0.0001) of control levels, respectively. Relative to PBS, 10 mg/kg IP ONT-89 resulted in significant reductions of serum ApoB protein levels on days 17, and 24 of the study, to 29% (p&lt;0.0001), and 23% (p&lt;0.0001) of control levels, respectively. 
     
       
         
           
               
             
               
                 TABLE E-28d 
               
             
            
               
                   
               
               
                 Serum Human Apolipoprotein B Levels Relative to PBS After 
               
               
                 Multiple IP Doses of 10 mg/kg Stereioisomer (ONT-87, 
               
               
                 -88 or -89) or Mipomersen in huApoB Mice (N = 3-4) 
               
            
           
           
               
               
               
            
               
                   
                 Day 
                   
               
            
           
           
               
               
               
               
            
               
                   
                 Sample 
                 17 
                 24 
               
               
                   
                   
               
               
                   
                 PBS 
                 100 ± 5  
                 100 ± 8     
               
               
                   
                 ONT-41 
                  44 z  ± 17 
                 27 z  ± 9 
               
               
                   
                 ONT-87 
                 27 az  ± 10 
                 14 z  ± 3 
               
               
                   
                 ONT-88 
                 23 az  ± 6  
                 17 z  ± 5 
               
               
                   
                 ONT-89 
                 29 az  ± 12 
                 23 z  ± 8 
               
               
                   
                   
               
               
                   
                   a Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.05 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   
                 b: Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.01 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   
                 c: Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   
                 d: Statistically different from the Mipomersen control group (ONT-41), with p &lt; 0.0001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   
                 w: Statistically different from the PBS control group, with p &lt; 0.05 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   
                 x: Statistically different from the PBS control group, with p &lt; 0.01 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   
                 y: Statistically different from the PBS control group, with p &lt; 0.001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
               
                   
                   z Statistically different from the PBS control group, with p &lt; 0.0001 (2-way ANOVA with Newman-Keuls post-hoc test) 
               
            
           
         
       
     
     Compared with 10 mg/kg IP Mipomersen, ONT-87 administered with the same dosing paradigm resulted in significantly higher reduction (knockdown) of serum ApoB protein levels on day 17, (p&lt;0.05) of the study. Compared with 10 mg/kg IP Mipomersen, ONT-88 administered with the same dosing paradigm resulted in significantly higher reduction (knockdown) of serum ApoB protein levels on day 17 (p&lt;0.05) of the study. Compared with 10 mg/kg IP Mipomersen, ONT-89 administered with the same dosing paradigm resulted in significantly higher reduction (knockdown) of serum ApoB protein levels on day 17 (p&lt;0.05) of the study. 
     Thus, in at least some embodiments, the present invention provides chirally controlled oligonucleotide compositions that show a biological activity and are characterized by extended persistence and/or slower rate of decay of that activity over time as compared with an appropriate reference (e.g., a preparation of oligonucleotides of the same sequence but different chiral specificity, including particularly stereorandom preparations). In some embodiments where such extended persistence and/or slower rate of decay is observed, provided chirally controlled compositions may be administered according to a dosing regimen with fewer total doses and/or longer periods between two or more doses that is utilized with the “parent” stereorandom composition to achieve comparable biologic and/or therapeutic effect. For example, the slower return to baseline of serum ApoB protein following treatment with chirally pure oligonucleotide compared with Mipomersen as demonstrated in the present Example, suggests that chirally pure ApoB oligonucleotides can be dosed less frequently than the parental stereorandom mixture (e.g., than Mipomersen). 
     The results presented in this Example demonstrate, for instance, that chirally pure oligonucleotide compositions can have significantly different pharmacological activity in vivo as compared with an appropriate reference (e.g., a preparation of oligonucleotides of the same sequence but different chiral specificity, including particularly stereorandom preparations), and specifically as compared with a “parental” stereorandom preparation. Those skilled in the art, in light of this demonstration, will appreciate that chirally controlled oligonucleotide compositions provided by the present disclosure have unexpected activities and characteristics. Those skilled in the art, in light of the present disclosure, will further appreciate that various methodologies are provided, including therapeutic methods that utilize dosing regimens that differ from those utilized for non-chirally controlled compositions (e.g., of the same nucleotide sequence). In some embodiments, relatively larger individual doses of chirally controlled (e.g., chirally pure) oligonucleotides may be utilized as compared with a control (e.g., a stereorandom control). In some embodiments, relatively smaller individual doses of chirally controlled (e.g., chirally pure) oligonucleotides may be utilized as compared with a control (e.g., a stereorandom control). In some embodiments, relatively fewer doses of chirally controlled (e.g., chirally pure) oligonucleotides may be utilized as compared with a control (e.g., a stereorandom control) within a given period of time. 
     Example 70: siRNA Agents Comprising Chirally Controlled Oligonucleotides Inhibit PCSK-9 
     The present Example demonstrates successful inhibition of target gene expression using siRNA agents comprised of chirally controlled oligonucleotides as described herein. Specifically, this Example describes hybridization of individual oligonucleotide strands prepared through chirally controlled synthesis as described herein, so that double-stranded chirally controlled siRNA oligonucleotide compositions are provided. This Example further demonstrates successful transfection of cells with such agents and, moreover, successful inhibition of target gene expression. 
     siRNA Transfection of Chiral siRNA Molecules 
     Hep3B, or HeLa cells were reverse transfected at a density of 2.0×10 4  cells/well in 96-well plates. Transfection of siRNA was carried out with lipofectamine RNAiMax (Life Technologies, cat. No. 13778-150) using the manufacturer&#39;s protocol, except with a decreased amount of Lipofectamine RNAiMax of 0.2 ul per well. Twelve, 1:3 siRNA duplex dilutions were created starting at 1 uM. 10 ul of 10×siRNA duplex was then lipoplexed with a prepared mixture of 9.8 ul of serum-free medium and 0.2 ul of Lipofectamine RNAiMax per well. After a 10-15 minute incubation, 2.0×10 4  cells in 80 ul of EMEM cell growing media (ATCC, 30-2003) were added to bring the final volume to 100 ul per well. Two separate transfection events were performed for each dose. 
     24 hours after transfection Hep3B or HeLa cells were lysed and PCSK9 mRNA was purified using MagMAX™-96 Total RNA Isolation Kit (Life Technologies, AM1830); 15 ul of cDNA was synthesized with High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Life Technologies, 4374967). Gene expression was evaluated by Real-Time PCR on a Lightcycler 480 (Roche) using a Probes Master Mix (Roche, 04 707 494 001) according to manufacturer&#39;s protocol using a FAM-labeled Taqman probe-set for PCSK9 (Life Technologies, Hs03037355_m1) and a VIC-labeled GAPDH primer-limited endogenous control (Life Technologies, NM_002046.3) 
     IC50s and Data Analysis 
     Delta delta Ct method was used to calculate values. Samples were normalized to hGAPDH and calibrated to mock transfected and untreated samples. A stereo-random molecule was used as positive control. The data are represented as a mean of 2 biological replicates using Graphpad Prism. A four-parameter linear regression curve was fitted to the data and the bottom and top were constrained to a 0 and 100 constants respectively in order to calculate a relative IC50. 
     
       
         
           
               
             
               
                 TABLE E-29 
               
             
            
               
                   
               
               
                 Relative IC50 values [Hep3B Transfection] 
               
            
           
           
               
               
               
               
            
               
                   
                 LogIC50 
                 HillSlope 
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 Bottom 
                 Top 
                 Mean 
                 SEM 
                 Mean 
                 SEM 
                 IC50 [nM] 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 ONT-D1 
                 0 
                 100 
                 −0.39 
                 0.12 
                 −0.27 
                 0.02 
                 0.41 
               
               
                 ONT-D2 
                 0 
                 100 
                 −0.85 
                 0.14 
                 −0.24 
                 0.03 
                 0.14 
               
               
                 ONT-D3 
                 0 
                 100 
                 −0.62 
                 0.14 
                 −0.24 
                 0.02 
                 0.24 
               
               
                 ONT-D4 
                 0 
                 100 
                 −0.49 
                 0.09 
                 −0.28 
                 0.02 
                 0.33 
               
               
                 ONT-D5 
                 0 
                 100 
                 −0.32 
                 0.09 
                 −0.31 
                 0.02 
                 0.48 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE E-30 
               
             
            
               
                   
               
               
                 Relative IC50 values [HeLa Transfection] 
               
            
           
           
               
               
               
               
            
               
                   
                 LogIC50 
                 HillSlope 
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 Bottom 
                 Top 
                 Mean 
                 SEM 
                 Mean 
                 SEM 
                 IC50 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 ONT-D1 
                 0 
                 100 
                 −1.73 
                 0.07 
                 −0.61 
                 0.05 
                 0.02 
               
               
                 ONT-D2 
                 0 
                 100 
                 −1.94 
                 0.07 
                 −0.66 
                 0.07 
                 0.01 
               
               
                 ONT-D3 
                 0 
                 100 
                 −1.72 
                 0.08 
                 −0.62 
                 0.07 
                 0.02 
               
               
                 ONT-D4 
                 0 
                 100 
                 −1.26 
                 0.06 
                 −0.74 
                 0.06 
                 0.06 
               
               
                 ONT-D5 
                 0 
                 100 
                 −1.03 
                 0.08 
                 −0.86 
                 0.12 
                 0.09 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE E-31 
               
             
            
               
                   
               
               
                 Relative IC50 values of siRNAs with 3 Phosporothioate 
               
               
                 stereo-centers[HeLa Transfection] 
               
            
           
           
               
               
               
               
            
               
                   
                 LogIC50 
                 HillSlope 
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 Bottom 
                 Top 
                 Mean 
                 SEM 
                 Mean 
                 SEM 
                 IC50 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 ONT-D7 
                 0 
                 100 
                 0.75 
                 0.15 
                 −0.24 
                 0.02 
                 5.60 
               
               
                 ONT-D8 
                 0 
                 100 
                 0.39 
                 0.09 
                 −0.32 
                 0.02 
                 2.45 
               
               
                 ONT-D9 
                 0 
                 100 
                 0.43 
                 0.13 
                 −0.22 
                 0.02 
                 2.68 
               
               
                 ONT-D10 
                 0 
                 100 
                 1.57 
                 0.20 
                 −0.27 
                 0.03 
                 36.86 
               
               
                 ONT-D11 
                 0 
                 100 
                 2.92 
                 0.33 
                 −0.12 
                 0.01 
                 839.42 
               
               
                 ONT-D12 
                 0 
                 100 
                 −0.17 
                 0.10 
                 −0.38 
                 0.03 
                 0.68 
               
               
                 ONT-D13 
                 0 
                 100 
                 1.20 
                 0.21 
                 −0.39 
                 0.08 
                 15.97 
               
               
                 ONT-D14 
                 0 
                 100 
                 −0.22 
                 0.18 
                 −0.43 
                 0.07 
                 0.60 
               
               
                 ONT-D15 
                 0 
                 100 
                 −0.25 
                 0.16 
                 −0.41 
                 0.06 
                 0.57 
               
               
                   
               
            
           
         
       
     
       FIGS.  45 - 50    present results of these studies.  FIG.  45    shows the % PCSK-9 mRNA remaining after Hep3B treatment with siRNA duplex.  FIG.  46    shows the % PCSK-9 mRNA remaining after Hep3B treatment with siRNA duplex curve fit.  FIG.  47    shows the % PCSK-9 mRNA remaining after HeLa treatment with siRNA duplex.  FIG.  48    shows the % PCSK-9 mRNA remaining after HeLa treatment with siRNA duplex curve fit.  FIG.  49    shows the % PCSK-9 mRNA remaining after HeLa treatment with siRNA duplex containing 3 Phosphorothiate stereo-centers.  FIG.  50    shows the % PCSK-9 mRNA remaining after HeLa treatment with siRNA duplex containing 3 Phosphorothiate stereo-centers curve fit. 
     As can be seen, in molecules with a single stereochemically defined phosphorothioate in each strand, a slightly increased potency was observed with molecules with Sp stereo-chemistry at the phosphorothioate in both sense and antisense strands While the specifically exemplified siRNA agents contained only one chiral center per strand, those skilled in the art, reading the present disclosure, will recognize the significance of the demonstrated differential effect as proof of principle that presence of chirality can impact activity. 
     The impact of chirality is more pronounced in siRNAs with more than two stereo-centers. In siRNAs with 3 chiral phosphorothioates (one in the sense strand and two in the antisense strand), an Sp stereochemistry at the 5′ end (between nucleotides n1 and n2) combined with a Rp stereochemistry at the 3′ end (between nucleotides n20 and n21) of the antisense strand had deleterious effect on potency. However, an Rp stereochemistry at the 5′ end (between nucleotides n1 and n2) combined with a Sp stereochemistry at the 3′ end (between nucleotides n20 and n21) of the antisense strand showed a significant PCSK-9 mRNA knockdown improvement over stereo-random control siRNA. The results suggest that both strands are affected by the stereochemistry of the phosphorothioate. It is likely that the observed efficacy differential will increase for agents with larger numbers of chiral centers and, moreover that both location and type of chiral center can impact activity, for example through effects on stability, potency, and/or both. Those skilled in the art, reading the present disclosure, will therefore appreciate that it provides teachings of both single and double-stranded chirally controlled oligonucleotide compositions, and uses thereof that, in at least some embodiments, distinguish such chirally controlled oligonucleotides and agents from stereorandom agents of identical sequence. 
     Example 71. Additional Exemplary Oligonucleotides and Synthesis Thereof 
     
       
         
         
             
             
         
       
     
     Oligonucleotides N101-N104 were synthesized using the automated synthesis on ABI-394 DNA/RNA synthesizer according to the synthetic cycle summarized on Table E-32, using 1 μmol synthesis column and 1.7 μmol of oxalyl linked dG CE,Pac  on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). After completion of the automated oligonucleotide synthesis, the HCP support was washed with dry ACN and dried under vacuum. The dry HCP was placed in a plastic vial and was treated with 1 mL of sat. NH 3 /Py for 1 h at 55° C. then 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for 18 h at RT. The solvents were then evaporated and the residue was re-suspended with ˜pH 1.5 HCl aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product was purified by reverse phase preparative HPLC (According to the procedure described below). The fractions having purity above 95% were pooled, concentrated and desalted by reverse-phase HPLC (According to the procedure described below). The final desalted product was lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-32 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of oligonucleotides N101-N102. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                   
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in 
                   
                   
               
               
                   
                   
                 ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6- 
                 20 
                 60 
               
               
                   
                   
                 lutidine 
                   
                   
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6- 
                 20 
                 60 
               
               
                   
                   
                 lutidine + 16% NMI in 
                   
                   
               
               
                   
                   
                 THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-(N- 
                 7 + 1.5 × 4 
                 360 + 3 × 180 + 
               
               
                   
                   
                 methylpiperazinoethyl- 
                   
                 900 
               
               
                   
                   
                 ToluylThioSulfonate 
                   
                   
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 72. HPLC Method for N101 and N102 
     Buffer A: 50 mM TEAA, pH 9.6 (adjusted with TEA) 
     Buffer B: ACN 
     Column: XBridge C 8 , 3.5 μm, 4.6×50 mm, Part #186003034 
     Buffer heater set temperature=35° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time 
                 Flow (ml/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 99 
                 1 
                   
               
               
                 2 
                 1 
                 99 
                 1 
                 1 
               
               
                 22 
                 1 
                 70 
                 30 
                 6 
               
               
                 25 
                 1 
                 5 
                 95 
                 6 
               
               
                 25.5 
                 1 
                 5 
                 95 
                 6 
               
               
                 30 
                 1 
                 99 
                 1 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 73. General UPLC-LCMS Method for N101 and N102 
     Buffer A: 10 mM ammonium formate, pH 9.5 (adjusted with NH 3  aq.) 
     Buffer B: ACN 
     Column: UPLC@OST C 18  1.7 μm, 2.1×500 mm 
     Column temperature=35° C. 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                 0.5 
                 99 
                 1 
                   
               
               
                 2 
                 0.5 
                 99 
                 1 
                 1 
               
               
                 12 
                 0.5 
                 60 
                 40 
                 6 
               
               
                 13 
                 0.5 
                 5 
                 95 
                 6 
               
               
                 13.5 
                 0.5 
                 5 
                 95 
                 6 
               
               
                 15 
                 0.5 
                 99 
                 1 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 74. General Purification Method for N101 and N102 
     Buffer A: 50 mM TEAA pH=9.6 (adjusted with TEA) 
     Buffer B: ACN 
     Column: XBridge Prep C 18 , 5 μm, C 18 , 250×10 mm, Part #186003256 
     Buffer heater set temperature=RT 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
                                             Time   Flow (ml/min)   % A   % B   Curve                                                    Initial       99   1           5   4   99   1   1       10   4   95   5   6       30   4   74   26   6       35   4   5   95   6       36   4   5   95   6       38   4   99   1   6       45   4   99   1   1                    
100 μL of 49-51% phosphoric acid was added to each fraction (pH 3) and checked by HPLC, then stored at −80° C. until frozen. The fractions were kept on lypholizer for a couple of hours until ½ volumes then stored at −80° C.
 
     Example 75. General Desalting Method for N101 and N102 
     Buffer A: 10 mM formic acid 
     Buffer B: ACN 
     Column: XBridge Prep C 18 , 5 μm, C 18 , 250×10 mm, Part #186003256 
     Buffer heater set temperature=RT 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
                                             Time   Flow (ml/min)   % A   % B   Curve                                                    Initial       100   0           12   4   100   0   1       13   4   65   35   6       21   4   65   35   1       21.5   4   100   0   6       30   4   100   0   1                    
Collected fractions were stored in −80° C. until frozen and kept on lypholizer until dryness and checked by HPLC.
 
     Example 76. Synthesis of Oligonucleotide N101 (All-Rp)-d[5mCs16As16Gs16T] 
     
       
         
         
             
             
         
       
     
     Oligonucleotide N101 was synthesized as described above and purified. RT in RP-HPLC: (HPLC method N1): 15.9 min. UPLC/ESI-MS: Calcd: 1614.64; Found [+H]: 1615.06. 
     Example 77. Synthesis of Oligonucleotide N102 (All-(Sp)-d[5mCs16As16Gs16T]) 
     Oligonucleotide N102 was synthesized as described above and purified. RT in RP-HPLC (HPLC method N1): 16.4 min. UPLC/ESI-MS: Calcd: 1614.64; Found [+H]: 1614.97. 
     Example 78. Synthesis of Oligonucleotide N103 (All-Rp)-d[5mCs16As16Gs16Ts165mCs16Ts16Gs165mCs16Ts16Ts165mCs16G] (SEQ ID NO: 125)) 
     Oligonucleotide N103 was synthesized as described above. RT in RP-HPLC (HPLC method N1): 18.4 min. UPLC/ESI-MS: Calcd for C 197 H 311 N 62 O 62 P 11 S 11 : 5233.38; Found: 5234.7. 
     Example 79. Synthesis of Oligonucleotide N104 (All-(Sp)-d[5mCs16As16Gs16Ts165mCs16Ts16Gs165mCs16Ts16Ts165mCs16G] (SEQ ID NO: 125)) 
     Oligonucleotide N104 was synthesized as described above. RT in RP-HPLC (HPLC method N1): 18.7 min. UPLC/ESI-MS: Calcd: 5233.38; Found: 5232.9. 
     Oligonucleotides N105-N106 were synthesized using the automated synthesis on ABI-394 DNA/RNA synthesizer according to the synthetic cycle summarized on Table E-33, using 1 μmol synthesis column and 1.7 μmol of oxalyl linked dG CE,Pac  on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). After completion of the automated oligonucleotide synthesis, the HCP support was washed with dry ACN and dried under vacuum. The dry HCP was placed in a plastic vial and was treated with 1 mL of sat. NH 3 /i-PrOH for 16 h at 55° C. The solvent was then evaporated and the residue was re-suspended with pH 7.0 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product is purified by reverse phase preparative HPLC. The fractions having purity above 95% are pooled, concentrated and desalted by reverse-phase HPLC. The final desalted product is lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-33 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of oligonucleotides N105-N106. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                   
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in 
                   
                   
               
               
                   
                   
                 ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6- 
                 20 
                 60 
               
               
                   
                   
                 lutidine 
                   
                   
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6- 
                 20 
                 60 
               
               
                   
                   
                 lutidine + 16% NMI in 
                   
                   
               
               
                   
                   
                 THF 
                   
                   
               
               
                   
               
               
                 5 
                 sulfurization 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 7 + 1.5 × 4 
                 360 + 3 × 180 + 900  
               
               
                   
               
            
           
         
       
     
     Example 80. HPLC Method for N101 and N102 
     Buffer A: 50 mM TEAA, pH 7.0 
     Buffer B: ACN 
     Column: XBridge C 8 , 3.5 μm, 4.6×150 mm, Part #186003034 
     Buffer heater set temperature=35° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time 
                 Flow (ml/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 99 
                 1 
                   
               
               
                 2 
                 1 
                 99 
                 1 
                 1 
               
               
                 22 
                 1 
                 80 
                 20 
                 6 
               
               
                 25 
                 1 
                 5 
                 95 
                 6 
               
               
                 25.5 
                 1 
                 5 
                 95 
                 6 
               
               
                 30 
                 1 
                 99 
                 1 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 81. General UPLC-LCMS Method for N101 and N102 
     Buffer A: 10 mM ammonium formate, pH 7.0 
     Buffer B: ACN 
     Column: UPLC@OST C 18  1.7 μm, 2.1×500 mm 
     Column temperature=35° C. 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                 0.5 
                 99 
                 1 
                   
               
               
                 2 
                 0.5 
                 99 
                 1 
                 1 
               
               
                 12 
                 0.5 
                 70 
                 30 
                 6 
               
               
                 13 
                 0.5 
                 5 
                 95 
                 6 
               
               
                 13.5 
                 0.5 
                 5 
                 95 
                 6 
               
               
                 15 
                 0.5 
                 99 
                 1 
                 1 
               
               
                   
               
            
           
         
       
     
     Example 82. Synthesis of Oligonucleotide N105 (All-(Rp)-d[5mCs9As9Gs9T]) 
     
       
         
         
             
             
         
       
     
     Oligonucleotide N105 was synthesized as described above. RT in RP-HPLC: (HPLC method N2): 14.1 min. UPLC/ESI-MS: Calcd: 1977.78; Found [−H]: 1978.8. 
     Example 83. Synthesis of Oligonucleotide N106 (All-(Sp)-d[5mCs9As9Gs9T]) 
     Oligonucleotide N106 was synthesized and purified as described above. RT in RP-HPLC (HPLC method N2): 14.7 min. UPLC/ESI-MS: Calcd: 1977.78; Found [−H]: 1977.37. 
     Oligonucleotides xxx were synthesized using the automated synthesis on ABI-394 DNA/RNA synthesizer according to the synthetic cycle summarized on Table E-33, using 1 μmol synthesis column and 1.7 μmol of oxalyl linked dG CE,Pac  on HCP. The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). After completion of the automated oligonucleotide synthesis, the HCP support was washed with dry ACN and dried under vacuum. The dry HCP was placed in a plastic vial and was treated with 1 mL of sat. NH 3 /i-PrOH for 16 h at 55° C. The solvent was then evaporated and the residue was re-suspended with pH 7.0 aqueous solution containing 10% DMSO and the HCP support was filtered off. The crude product is purified by reverse phase preparative HPLC. The fractions having purity above 95% are pooled, concentrated and desalted by reverse-phase HPLC. The final desalted product is lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-34 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA 
               
               
                 Synthesizer ABI-394. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                   
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in 
                   
                   
               
               
                   
                   
                 ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6- 
                 20 
                 60 
               
               
                   
                   
                 lutidine 
                   
                   
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6- 
                 20 
                 60 
               
               
                   
                   
                 lutidine + 16% NMI in 
                   
                   
               
               
                   
                   
                 THF 
                   
                   
               
               
                   
               
               
                 5 
                 sulfurization 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 10 + 4 × 2 
                 300 + 3 × 150 + 600  
               
               
                   
               
            
           
         
       
     
     Example 84. General Purification Method 
     Buffer A: 50 mM TEAA 
     Buffer B: ACN 
     Column: XBridge Prep C 18 , 5 μm, C 18 , 250×10 mm, Part #186003256 
     Buffer heater set temperature=rt 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
                                             Time   Flow (ml/min)   % A   % B   Curve                                                    Initial       99   1           5   4   99   1   1       10   4   95   5   6       30   4   74   26   6       35   4   5   95   6       36   4   5   95   6       38   4   99   1   6       45   4   99   1   1                    
HPLC Method 5:
 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time 
                 Flow (ml/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 80 
                 20 
                   
               
               
                 2 
                 1 
                 80 
                 20 
                 1 
               
               
                 22 
                 1 
                 45 
                 55 
                 6 
               
               
                 25 
                 1 
                 5 
                 95 
                 6 
               
               
                 25.5 
                 1 
                 5 
                 95 
                 6 
               
               
                 26 
                 1 
                 85 
                 20 
                 6 
               
               
                 30 
                 1 
                 85 
                 20 
                 1 
               
               
                   
               
            
           
         
       
     
     ONT-60: Oligonucleotide 149 All-(Rp)-d[5mCs8As8Gs8Ts85mCs8Ts8Gs85mCs8Ts8Ts85mCs8G] (SEQ ID NO: 125) 
     
       
         
         
             
             
         
       
     
     The oligonucleotide was synthesized as described above. RT in RP-HPLC (HPLC method 5): 15.80 min. UPLC/ESI-MS: Calcd: 6345.6; Found: 6340.7. 
     ONT-69: All-(Sp)-d[5mCs16As8Gs8Ts85mCs8Ts8Gs85mCs8Ts8Ts85mCs8G] (SEQ ID NO: 125) 
     The oligonucleotide was synthesized as described above. RT in RP-HPLC (HPLC method 5): 18.61 min. UPLC/ESI-MS: Calcd: 6345.6; Found: 6340.6. 
     Example 85 
     The P-modified blockmer and altmer oligonucleotides containing both stereodefined phosphorothioate diester and stereodefined morpholinoethyl phosphorothioate triester internucleotidic linkages were synthesized on an ABI-394 DNA/RNA synthesizer according to the cycle summarized in Table E-4 using 1 μmol synthesis column and 1.7 μmol of oxalyl linked dG CE,Pac  on HCP. Either stereodefined P-modified phosphorothioate linkage was introduced at predetermined positions within the sequence by performing either sulfurization step (1) or (2). The synthesis cycle was performed with removal of the terminal 5′-O-DMTr group (DMT Off). The solid support was washed with dry ACN and dried under a flux of argon. The dry HCP was placed in a plastic vial and was treated with 1 mL of dry propylamine in dry pyridine (in a 1:4 ratio) for a period of 18 h at r.t. The solvents were then evaporated and the residue was re-suspended in DMSO and the HCP support was filtered off. The crude product was purified by reverse phase preparative HPLC. The fractions having purity above 95% were pooled, concentrated and desalted by reverse-phase HPLC (Gradient of 0 to 80% ACN). The final desalted product was lyophilized from water. 
     
       
         
           
               
             
               
                 TABLE E-4 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394 
               
               
                 Used for the Synthesis of Example 85. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                   
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 
                 20 
                 60 
               
               
                   
                   
                 16% NMI in THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-Morpholinoethyl p- 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                   
                 Chlorophenyl thiosulfonate 
                   
                 600  
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                   
                   
               
               
                   
               
               
                 6 
                 sulfurization 
                 0.3M S-cyanoethyl 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                   
                 Methylthiosulfonate 
                   
                 600  
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 86. General Purification Method for Example 85 
     Buffer A: 20 mM Phosphate pH=6.0 (adjusted with phosphoric acid) 
     Buffer B: ACN 
     Column: XBridge Prep C 18 , 5 μm, C 18 , 250×10 mm, Part #186003256 
     Buffer heater set temperature=50° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
                                             Time   Flow (ml/min)   % A   % B   Curve                                                    Initial       99   1           5   4   99   1   1       10   4   77   23   6       60   4   70   30   6       65   4   20   80   6       70   4   20   80   6       71   4   99   1   6       80   4   99   1   1                    
HPLC Method 6
 
Buffer A: 20 mM Ammonium acetate, pH 6.0
 
Buffer B: ACN
 
Column: XBridge C 8 , 3.5 μm, 4.6×150 mm, Part #186003034
 
Buffer heater set temperature=60° C.
 
Signal monitored at 254 and 280 nm
 
Gradient used:
 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time 
                 Flow (ml/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 90 
                 10 
                   
               
               
                 2 
                 1 
                 90 
                 10 
                 1 
               
               
                 22 
                 1 
                 75 
                 25 
                 6 
               
               
                 25 
                 1 
                 5 
                 95 
                 6 
               
               
                 25 
                 1 
                 5 
                 95 
                 6 
               
               
                 25.5 
                 1 
                 90 
                 10 
                 6 
               
               
                 30 
                 1 
                 90 
                 10 
                 1 
               
               
                   
               
            
           
         
       
     
     ONT-71: All-(Rp)-d[5mCs1As1GsTs5mCsTsGs5mCsTsTs1S5mCs1G] (SEQ ID NO: 125) 
     The oligonucleotide was synthesized as described above. RT in RP-HPLC (HPLC method 6): 9.39 min. UPLC/ESI-MS: Calcd: 4297.9; Found: 4295.3 
     ONT-72: All-(Sp)-d[5mCs1As1GsTs5mCsTsGs5mCsTsTs15mCs1G] (SEQ ID NO: 125) 
     The oligonucleotide was synthesized as described above. RT in RP-HPLC (HPLC method 6): 10.84 min. UPLC/ESI-MS: Calcd: 4297.9; Found: 4295.7 
     ONT-73: All-(Rp)-d[5mCs1AsGs1Ts5mCs1TsGs15mCsTs1Ts5mCs1G] (SEQ ID NO: 125) 
     The oligonucleotide was synthesized as described above. RT in RP-HPLC (HPLC method 6): 13.54 min. UPLC/ESI-MS: Calcd: 4524.2; Found: 4522.6 
     ONT-74: All-(Sp)-d[5mCs1AsGs1Ts5mCs1TsGs15mCsTs1Ts5mCs1G] (SEQ ID NO: 125) 
     The oligonucleotide was synthesized as described above. RT in RP-HPLC (HPLC method 6): 15.52 min. UPLC/ESI-MS: Calcd: 4524.2; Found: 4521.0 
     Prodrug oligonucleotide properties such as: nuclease resistance, tissue accumulation, cell penetration, endosomal escape, immunostimulation, duration of action, pharmacokinetics, etc. are all modulated and influenced by the stereochemistry of the chiral phosphorothioate backbone linkages. 
     RNA oligonucleotides containing 2′-OH, 2′-OMe, 2′-F, 2′-deoxy, internucleotidic phosphodiester or internucleotidic stereodefined phosphorothioate diester or internucleotidic stereodefined phosphorothioate triester (ProDrug) (either releasing a internucleotidic phosphodiester (PO) or a internucleotidic stereodefined phosphorothioate diester (PS)) linkages are synthesized on ABI 394 DNA/RNA synthesizer according to the cycles summarized in Table E-35, Table E-36, Table E-37 and Table E-38, using a 10 μmol capacity synthesis column loaded with 130 mg (4.9 μmol) of oxalyl linked 5′-O-DMTr-2′-deoxythymidine prepared as previously described. Prior to synthesis, a preliminary capping step (capping 2) is performed and the synthesis is terminated with removal of the terminal 5′-O-DMTr groups. The oxidation step is performed using a commercially available 5-6 M solution of tert-butyl hydroperoxide (TBHP) in decane which was then diluted with four parts dichloromethane. The stereospecific sulfurization step for internucleotidic stereodefined phosphorothioate diester linkage is performed using the 0.3 M S-cyanoethylmethylthiosulfonate reagent following the coupling of the corresponding chiral phosphoramide and the two-step capping process (Table E-36). The stereospecific sulfurization step for internucleotidic stereodefined phosphorothioate triester releasing a stereodefined internucleotidic phosphodiester (PS) linkage is performed using the 0.3 M S—(N-morpholinoethylthioester-ethyl)-para-nitro-toluylthiosulfonate reagent following the coupling of the corresponding chiral phosphoramide and the two-step capping process (Table E-37). The stereospecific sulfurization step for internucleotidic stereodefined phosphorothioate triester releasing an internucleotidic phosphodiester (PO) linkage is performed using the 0.3 M S—(N-morpholinoethyl)-toluylthiosulfonate reagent following the coupling of the corresponding chiral phosphoramide and the two-step capping process (Table E-38). For 2′-OH RNA nucleotides, 2′-O-base protecting groups are used, such as 2′-O-PivOM (Debart et al.,  Chem. Eur. J.,  2008, 14, 9135) or 2′-O-TC (Dellinger et al.,  J. Am. Chem. Soc.,  2011, 133, 11540). Ultra-mild (C-Ac, G-Pac, A-Pac) protecting groups are used for all of the nucleobases. 
     Once the automated oligonucleotide synthesis cycle is completed and the final 5′-O-DMTr group is removed, the synthesis column is taken off the DNA/RNA synthesizer and dried under vacuum. 10 mL solution of 0.5 M 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 0.25 M N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) in ACN is continuously added to the support through the synthesis column for 1 min without stopping the flow using a syringe attached to one end of the synthesis column. The support is then washed with anhydrous ACN and dried under vacuum. Then, the dried support is transferred into an empty screw-cap plastic vial and treated with 10% n-PrNH 2  solution in anhydrous pyridine (1.5 mL) at room temperature for 12 h. After that, the solvents are evaporated to dryness and the residue including the solid support is dissolved in 2 mL of water/DMSO (50/50) at pH 5, the support is filtered off and the filtrates are collected, immediately frozen and stored at −80° C. prior to purification. 
     
       
         
           
               
             
               
                 TABLE E-35 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis 
               
               
                 on a DNA/RNA Synthesizer ABI 394 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery 
                 wait 
               
               
                 step 
                 reaction 
                 reagent 
                 time (sec) 
                 time (sec) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 120 + 10 
                 N. A. 
               
               
                   
                   
                 0.15M 
               
               
                 2 
                 coupling 
                 phosphoramidite in 
                 7 + 6 
                 30 + 600 
               
               
                   
                   
                 ACN + 2M CMPT in 
               
               
                   
                   
                 ACN 
               
               
                   
                   
                 5% Pac 2 O in THF/2,6- 
               
               
                 3 
                 capping 
                 lutidine + 16% NMI in 
                 10 
                 20 
               
               
                   
                   
                 THF 
               
               
                 4 
                 oxidation 
                 1.1M tert-butyl 
                 20 
                 110 
               
               
                   
                   
                 hydroperoxide in 4:1 
               
               
                   
                   
                 dichloromethane:decane 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE E-36 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI 394 
               
            
           
           
               
               
               
               
               
            
               
                 step 
                 reaction 
                 reagent 
                 delivery time (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 120 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M chiral 
                 8 + 6 
                 30 + 900 (2′-OMe 
               
               
                   
                   
                 phosphoramidite in 
                   
                 RNA) 
               
               
                   
                   
                 ACN + 2M CMPT in 
                   
                 30 + 600 (DNA) 
               
               
                   
                   
                 ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6- 
                 30 
                 60 
               
               
                   
                   
                 lutidine 
                   
                   
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6- 
                 30 
                 60 
               
               
                   
                   
                 lutidine + 16% NMI in 
                   
                   
               
               
                   
                   
                 THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-(2-cyanoethyl) 
                 15 + 3 × 4 
                 120 + 3 × 60 + 300 
               
               
                   
                   
                 methylthiosulfonate 
                   
                   
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE E-37 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                   
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in 
                   
                   
               
               
                   
                   
                 ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6- 
                 20 
                 60 
               
               
                   
                   
                 lutidine 
                   
                   
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6- 
                 20 
                 60 
               
               
                   
                   
                 lutidine + 16% NMI in 
                   
                   
               
               
                   
                   
                 THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-(N- 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                   
                 morpholinothioester-ethyl- 
                   
                 600  
               
               
                   
                   
                 p-nitro-toluylthiosulfonate 
                   
                   
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE E-38 
               
             
            
               
                   
               
               
                 Summary for Oligonucleotide Synthesis on a DNA/RNA Synthesizer ABI-394. 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 delivery time 
                   
               
               
                 step 
                 reaction 
                 reagent 
                 (sec) 
                 wait time (sec) 
               
               
                   
               
               
                 1 
                 detritylation 
                 3% TCA in DCM 
                 3 + 60 + 10 
                 N.A. 
               
               
                 2 
                 coupling 
                 0.15M phosphoramidite in 
                 5 + 4 
                 30 + 600 
               
               
                   
                   
                 ACN + 1.2M CMPT in ACN 
                   
                   
               
               
                 3 
                 capping 1 
                 5% Pac 2 O in THF/2,6-lutidine 
                 20 
                 60 
               
               
                 4 
                 capping 2 
                 5% Pac 2 O in THF/2,6-lutidine + 
                 20 
                 60 
               
               
                   
                   
                 16% NMI in THF 
                   
                   
               
               
                 5 
                 sulfurization 
                 0.3M S-Morpholinoethyl 
                 10 + 4 × 2 
                 300 + 3 × 150 + 
               
               
                   
                   
                 Toluylthiosulfonate 
                   
                 600 
               
               
                   
               
               
                   
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
         
             
             
         
       
     
     Synthesis of Oligonucleotide: (Rp)-uucuAGAccuGuuuuGcuudTs10dT (SEQ ID NO: 121) 
     Synthesis of Oligonucleotide ONT-107: (Sp)-uucuAGAccuGuuuuGcuudTs10dT (SEQ ID NO: 121) 
     Synthesis of Oligonucleotide ONT-108: (Rp)-AAGcAAAAcAGGUCuAGAAdTs10dT (SEQ ID NO: 122) 
     Synthesis of Oligonucleotide ONT-109: (Sp)-AAGcAAAAcAGGUCuAGAAdTs10dT (SEQ ID NO: 122) 
     
       
         
         
             
             
         
       
     
     Synthesis of Oligonucleotide: (Rp, Rp)-as1AGcAAAAcAGGUCuAGAAdTsdT (SEQ ID NO: 122) 
     Synthesis of Oligonucleotide: (Sp, Rp)-as1GcAAAAcAGGUCuAGAAdTsdT (SEQ ID NO: 123) 
     Synthesis of Oligonucleotide: (Sp, Sp)-as1GcAAAAcAGGUCuAGAAdTsdT (SEQ ID NO: 123) 
     Synthesis of Oligonucleotide: (Rp, Sp)-as1GcAAAAcAGGUCuAGAAdTsdT (SEQ ID NO: 123) 
     Synthesis of Oligonucleotide: (All (Sp))-us1ucus1AGAccs1uGus1uus1uGcuudTs10dT (SEQ ID NO: 121) 
     Synthesis of Oligonucleotide: (All (Rp))-As10AGcAAAAcAGGs1UCuAs1GAs1AdTs10dT (SEQ ID NO: 122) 
     RNA strand thermal annealing and preparation of siRNA duplexes. Each RNA strand is mixed with its complementary RNA strand in equimolar concentration of 10 μM in 1×PBS. Total 0.5 mL solution is prepared for each duplex and the mixture is heated at 90° C. for 2 min and is allowed to cool down over the course of several hours. The mixtures are then stored at 4° C. 
     Following the thermal RNA strand annealing step, all the possible siRNA duplex combinations are prepared by annealing any of the Sense strands with any possible complementary strand of the Antisense strands. 
     All prepared siRNA duplexes are evaluated in vitro for their PCSK9 gene-silencing properties, following transfection in HeLa cells or Hep3B cells. 
     All prepared siRNA duplexes with ProDrug groups are evaluated in vitro for their PCSK9 gene-silencing properties, following free uptake in Hep3B cells, Huh-7 cells or human primary hepatocytes. 
     Different potencies are observed, modulated by the number, the position and the stereo architecture of the chiral phosphorothioate backbone linkages, combined with the layers of additional chemical modifications and ProDrug groups explored. 
     siRNA properties such as: nuclease resistance, cell penetration, endosomal escape, duplex thermodynamic stability, tridemnsional structure of the duplex, affinity towards the various mechanistic steps of enzyme interactions, affinity towards the target mRNA, specific off-target effects, immunostimulation, duration of action, pharmacokinetics, etc. are all modulated and influenced by the stereochemistry of the chiral phosphorothioate backbone linkages as well as the presence or the absence of the ProDrug group. 
     The attachment of PO and PS releasing ProDrug groups to the siRNA duplexes enhances their intracellular delivery and free uptake in the absence of transfection reagent or targeting ligand. 
     Example 87: Stability Studies of Diastereomerically Pure Oligonucleotides 
     The present Example compares the in vitro stability of chirally pure oligonucleotides with that observed for the “parent” stereorandom mixture (i.e., for a composition containing oligonucleotides of the same sequence as the chirally pure oligonucleotides but not displaying chiral purity, for example as a result of having been prepared via a stereorandom process). Seven chirally pure oligonucleotides, each of which had a sequence complementary to that of a particular target transcript or gene encoding a protein of interest were synthesized, formulated, and assessed for metabolic stability using three different biological matrices: snake venom phosphodiesterase (svPDE), nuclease P1 (nP1) and rat whole liver homogenates. Levels of full-length oligonucleotide were quantified by IEX-HPLC after different incubation periods. The results presented in this Example demonstrate, for instance, that chirally pure oligonucleotide compositions can have significantly different metabolic stability as compared with an appropriate reference (e.g., a preparation of oligonucleotides of the same sequence but different chiral specificity, including particularly stereorandom preparations), and specifically as compared with a “parental” stereorandom preparation. In this Example, oligonucleotides having a sequence antisense to (and therefore targeting) human Apolipoprotein-B (ApoB) were used for proof-of-concept in metabolic stability studies. 
     Snake Venom Phosphodiesterase (svPDE) Digestion Study for Oligonucleotides ONT-75, ONT-77, ONT-80, ONT-81, ONT-87, ONT-88, ONT-89 and ONT-41 
     We used the protocol reported by Oka et al. ( J. Am. Chem. Soc.  2008, 130, 16031-16037) with minor modifications. Purified oligonucleotide (10 nmoles) in water (50 μL) was added to the aqueous solution (450 μL, pH 8.6) containing svPDE (4×10 −3  units), 100 mM Tris-HCl and 15 mM MgCl 2 . The mixture was incubated at 37° C. with shaking at 400 rpm. A 50 μL aliquot was taken at each time point (0 h, 12 h, 1 d, 2 d, 3 d, 4 d, 5 d, 6 d and 7 d) and was quenched with 25 μL of 150 mM EDTA, 2 μL of Proteinase K solution (20 mg/mL) and 30 μL of Lysis buffer (Qiagen, #129115) and the mixture was heated at 60° C. for 20 min. 5 μL of internal standard (5′- GC GTT TGCTCTT CTTCTTGCGTTT TTT -3′ (SEQ ID NO: 126), a 27-mer oligonucleotide (underlined nucleotides are 2′-MOE modified), (200 μM) was added to the aliquot. Quantification analyses were performed by IEX-HPLC and metabolite identification was carried out by UPLC/MS. The results were illustrated in  FIG.  59   . 
     IEX-HPLC analysis showed degradation of phosphorothioate 20-mer during incubation with svPDE with no significant difference between stereoisomers. LCMS analysis of metabolites revealed that the majority of the degradation products were formed as a result of desulfurization. As reported previously by Prakash et al. ( Biochemistry  2002, 41, 11642-11648), Prhavc et al. ( Org. Lett.,  2003, 5, 2017-2020) and others, we also observed that 2′-MOE modifications at 5′ and 3′-ends protect these oligomers from svPDE digestion which is a 3′-5′ exonuclease. 
     Nuclease P1 (nP1) Digestion Study for Oligonucleotides ONT-75, ONT-77, ONT-80, ONT-81, ONT-87, ONT-88, ONT-89 and ONT-41 
     We employed the protocol reported by Oka et al. ( J. Am. Chem. Soc.  2008, 130, 16031-16037) with minor modifications. Purified oligonucleotide (10 nmoles) in Water (50 μL) was added to an aqueous solution (500 μL, pH 7.2) containing nP1 (20 units), 100 mM Tris-HCl and 1 mM ZnCl 2 . The mixture was incubated at 37° C. with shaking at 400 rpm. A 50 μL aliquot was taken at each time point (0 h, 1 h, 2 h, 4 h, 8 h, 12 h, 1 d, 2 d) and was quenched with 25 μL of stop buffer (150 mM EDTA, 2 μL of Proteinase K solution (20 mg/mL) and 30 μL of Lysis buffer (Qiagen, #129115) and the mixture was heated at 60° C. for 20 min. 5 μL of internal standard (5′- GC GTTT GCTCTT CTTCTTGCGTTTT TT -3′ (SEQ ID NO: 126)), a 27-mer oligonucleotide (underlined nucleotides are 2′-MOE modified) (200 μM) was added to the aliquot. Quantification analyses were performed by IEX-HPLC and metabolite identification was carried out by UPLC/MS. The results were illustrated in  FIGS.  60 - 67   . 
     Nuclease nP1 has previously been reported to specifically cleave DNA phosphorothioate linkages having an (Sp) absolute configuration at the phosphorus atoms (Porter et al.,  Biochemistry,  1983, 22, 1369-1377; Oka et al.,  J. Am. Chem. Soc.,  2008, 130, 16031-16037). We observed a similar pattern of cleavage for the 5-10-5 2′-MOE gapmer phosphorothioate oligonucleotides studied, where nP1 was found to digest efficiently the DNA core of the gapmer oligonucleotides at the (Sp) phosphorothioate centers, without affecting the 2′-MOE wings, which were stable independent of stereochemistry. The complete digestion of the DNA core was observed in one hour for the stereorandom diastereomixture oligonucleotide ONT-41, as well as for the stereopure oligonucleotides containing (Sp)-phosphorothioate internucleotidic linkages in the DNA core (ONT-77, ONT-80, ONT-87, ONT-88 and ONT-89). All products of cleavage were clearly identified by UPLC/MS. As previously reported in the literature, we found that (Rp) phosphorothioate DNA was clearly not a substrate for nP1. The two chirally pure oligonucleotides having (Rp) phosphorothioate DNA cores (ONT-75 and ONT-81) were completely stable to nP1 for an incubation period of 1 h at 37° C. ONT-75 and ONT-81 showed c.a. 10-15% loss of the full length products during the course of incubation over several days. The results presented in this Example demonstrate, for instance, that chirally pure oligonucleotide compositions can have significantly different metabolic stability in an nP1 assay as compared with an appropriate reference (e.g., a preparation of oligonucleotides of the same sequence but different chiral specificity, including particularly stereorandom preparations), and specifically as compared with a “parental” stereorandom preparation. 
     General IEX-HPLC Method for the Analysis of Enzyme Digestion Products 
     Buffer A: 10 mM TrisHCl, 50% ACN, pH 8.0 
     Buffer B: 10 mM TrisHCl, 800 mM NaClO 4 , 50% ACN, pH 8.0 
     Column: DIONEX, DNAPac, PA-100, 4.0×250 mm, Part #063000 
     Column temperature=60° C. 
     Signal monitored at 254 and 280 nm 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                   
                 95 
                 5 
                   
               
               
                 2 
                 1 
                 95 
                 5 
                 1 
               
               
                 3 
                 1 
                 75 
                 25 
                 6 
               
               
                 10 
                 1 
                 35 
                 65 
                 6 
               
               
                 10.1 
                 1 
                 95 
                 5 
                 6 
               
               
                 12.5 
                 1 
                 95 
                 5 
                 1 
               
               
                   
               
            
           
         
       
     
     General UPLC-LCMS Method for the Analysis of Enzyme Digestion Products. 
     Buffer A: 15 mM TEA, 400 mM HFIP, Water 
     Buffer B: 50:50 Buffer A/Methanol 
     Column: UPLC@OST C 18  1.7 μm, 2.1×500 mm 
     Column temperature=60° C. 
     Gradient used: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                 Time (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 Curve 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Initial 
                 0.2 
                 70 
                 30 
                   
               
               
                 2 
                 0.2 
                 70 
                 30 
                 1 
               
               
                 27 
                 0.2 
                 35 
                 65 
                 6 
               
               
                 27.5 
                 0.2 
                 5 
                 95 
                 6 
               
               
                 28.5 
                 0.2 
                 5 
                 95 
                 6 
               
               
                 29 
                 0.2 
                 70 
                 30 
                 6 
               
               
                 30 
                 0.2 
                 70 
                 30 
                 1 
               
               
                   
               
            
           
         
       
     
     In Vitro Metabolic Stabilities of Human Diastereomerically Pure Oligonucleotides in Preincubated Rat Whole Liver Homogenates 
     The metabolic stability of chirally pure oligonucleotides in in vitro rat whole liver homogenates was measured. The protocol employed here has previously been reported and used to evaluate the stability of oligonucleotide drugs. 
     Protocol: The protocol reported by Geary et al was employed in the current study ( Oligonucleotides,  2010, 20, 309) with some modifications. 
     Test system: Two male Sprague-Dawley rats ( Rattus norvegicus ) were supplied by Charles River Laboratories, Inc., (Hollister, Calif.). 
     Tissue Collection: Animals were acclimated to the study room for two days prior to tissue collection. At the time of tissue collection, animals were anesthetized with an intraperitoneal (IP) injection of sodium pentobarbital solution. Liver perfusion was performed using 500 mL of chilled saline/animal, administered via the hepatic portal vein. After perfusion, the livers were dissected and maintained on ice. Livers were minced into small pieces then weighed. 
     Liver Homogenate Preparation: The minced pieces of liver tissues were transferred to tared 50 mL centrifuge tubes and weighed. Chilled homogenization buffer (100 mM Tris pH 8.0, 1 mM magnesium acetate, with antibiotic-antimycotic agents) was added to each tube, such that the tube(s) contained 5 mL of buffer per gram of tissue. Using a QIAGEN TissueRuptor tissue homogenizer, the liver/buffer mixture was homogenized while maintaining the tube on ice. The protein concentration of the liver homogenate was determined using a Pierce BCA protein assay. Liver homogenates were divided into 1 mL aliquots, transferred to cryovials and stored at −60° C. 
     Incubation Conditions: 1 mL aliquots of frozen liver homogenate (protein concentration=31.9 mg/mL) were thawed and incubated at 37° C. for 24 h. Six eppendorf tubes (2 mL) were taken and 450 μL of homogenate was added in each tube. 50 μL of test oligonucleotide (200 μM) was added to each tube. Immediately after mixing, 125 μL of (5×) stop buffer (2.5% IGEPAL, 0.5 M NaCl, 5 mM EDTA, 50 mM Tris, pH=8.0) and 12.5 μL of 20 mg/mL Proteinase K (Ambion, #AM2546) was added to one tube for a 0 hour time point. The mixture was then heated at 60° C. for one hour. The remaining reaction mixtures were incubated at 37° C. with shaking at 400 rpm on a VWR Incubating Microplate shaker. After incubation for a designated period (1, 2, 3, 4, and 5 days), each mixture was treated with 125 μL of (5×) stop buffer (2.5% IGEPAL, 0.5 M NaCl, 5 mM EDTA, 50 mM Tris, pH=8.0) and 12.5 μL of 20 mg/mL Proteinase K (Ambion, #AM2546). 
     Work up and Bioanalysis: (5′- GC GTTT GCTCTT CTTCTTGCGTTTT TT -3′ (SEQ ID NO: 126)), a 27-mer oligonucleotide (underlined nucleotides are 2′-MOE modified) was used as the internal standard for quantitation of diastereomerically pure oligonucleotides. 50 μL of internal standard (200 μM) was added to each tube followed by addition of 250 μL of 30% ammonium hydroxide, 800 μL of Phenol:Chloroform:isoamyl alcohol (25:24:1). After mixing and centrifugation at 600 rpm, the aqueous layer was evaporated on speed vac to 100 μL and loaded on a Sep Pak column (C18, 1 g, WAT 036905). All the aqueous washings of the Sep pak column were tested with quick IEX-HPLC method to ensure that no product was found there. The acetonitrile eluate was concentrated to dryness and dissolved in 100 μL water and analyzed using RP-HPLC. 
     Eluant A=10 mM Tributylammonium acetate, pH=7.0 
     Eluant B=ACN 
     Column: XTerra MS C 18 , 3.5 μm, 4.6×150 mm, Part number: 186000432 
     Column Temperature=60° C. 
     HPLC Gradient: 
     
       
         
           
               
               
               
               
               
               
             
               
                   
                   
               
               
                   
                 Time 
                 Flow 
                 % A 
                 % B 
                 Curve 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 1 
                   
                 1.0 
                 65 
                 35 
                   
               
               
                   
                 2 
                 5.0 
                 1.0 
                 65 
                 35 
                 1 
               
               
                   
                 3 
                 30.0 
                 1.0 
                 40 
                 60 
                 6 
               
               
                   
                 4 
                 35.0 
                 1.0 
                 5 
                 90 
                 6 
               
               
                   
                 5 
                 36.0 
                 1.0 
                 65 
                 35 
                 6 
               
               
                   
                 6 
                 40.0 
                 1.0 
                 65 
                 35 
                 1 
               
               
                   
                   
               
            
           
         
       
     
       FIG.  68    shows that different chirally pure oligonucleotides have different metabolic stability profiles in rat whole liver homogenate. The experiment also demonstrates that the diastereoisomeric mixture ONT-41 has a different metabolic stability profile compared with diastereomerically pure stereodefined oligonucleotides with the same sequence and chemical composition (ONT-75 and ONT-77 are used here as examples, but this observation, as appreciated by a person of ordinary skill in the art, may be extrapolated to other possible diastereomerically pure stereodefined phosphorothioate diastereoisomers of this molecule). 
     The oligonucleotide ONT-75, having a stereocontrolled phosphorothioate backbone with full Rp absolute configuration at all phosphorus atoms was degraded completely in one day in preincubated rat whole liver homogenates at 37° C., demonstrating the lowest metabolic stability of the three used in the study. 
     The other diastereomerically pure stereodefined phosphorothioate oligonucleotide ONT-77 (having absolute configuration: 5R-10S-4R) was more than two-fold more stable than the stereorandom ONT-41 (Mipomersen). After incubation for 5 days 40% of full length oligonucleotide remained for the diastereomerically pure ONT-77 vs. approximately 15% of full length oligonucleotide for the stereorandom ONT-41 (Mipomersen). A direct comparison between ONT-77 and ONT-41 clearly demonstrated a significantly higher metabolic stability for the stereocontrolled isomer 5R-10S-4R (ONT-77), throughout all of the analyzed time points. The stereodefined architecture of the diastereomerically pure isomer ONT-77 clearly affects the rate of degradation and the overall metabolic stability of this oligonucleotide. These observations lead to the conclusion, without the intention to be limited by theory, that the Sp stereochemistry applied to the DNA core of the 5-10-5 gapmer oligonucleotide provides enhanced endonuclease resistance and hence enhanced metabolic stability compared with the stereorandom diastereomixture. Some other stereo architectures used for other diastereomerically pure stereocontrolled phosphorothioate isomers of this sequence would be expected to show even higher metabolic stability in this experiment. 
     While not wishing to be limited by theory, the results presented in this Example demonstrate, for instance, that Rp stereochemistry at the phosphorothioate linkage (ONT-75) is less metabolically stable in rat liver homogenates than ONT-77, which contains the Sp DNA core. The results presented in this Example also demonstrate, for instance, that chirally pure oligonucleotide compositions can have significantly different metabolic stability in rat liver homogenate as compared with an appropriate reference (e.g., a preparation of oligonucleotides of the same sequence but different chiral specificity, including particularly stereorandom preparations), and specifically as compared with a “parental” stereorandom preparation. In this Example, oligonucleotides having a sequence antisense to (and therefore targeting) human Apolipoprotein-B (ApoB) were used for proof-of-concept in metabolic stability studies. A provided chirally controlled oligonucleotides may have increased or decreased stability towards endogenous enzymes. By providing chirally controlled oligonucleotide and compositions and methods thereof, the present invention provided oligonucleotides and compositions that have enhanced pharmacological properties than known chirally uncontrolled oligonucleotides and their compositions. 
     In Vitro Metabolic Stabilities of Human PCSK9 siRNA Duplexes Having Stereocontrolled Phosphorothioate Diester Linkages in Human Serum. 
     A protocol similar to previously reported procedures (Oka et al.,  J. Am. Chem. Soc.  2008, 130, 16031-16037) is used. siRNA duplexes (2.5 μmoles) in water (50 μL) are added to human serum (450 μL). The mixture is incubated at 37° C. with shaking at 400 rpm. 50 μL aliquots are taken out at each time point (0 h, 0.5 h, 1 h, 2 h, 4 h, 6 h, 8 h and 24 h) and are quenched with 25 μL of 150 mM EDTA, 2 μL of Proteinase K solution (20 mg/mL) and 30 μL of Lysis buffer (Qiagen, #129115) and the mixture is heated at 60° C. for 20 min. 5 μL of Internal standard (5′- GC GTT TGCTCTT CTTCTTGCGTTTT TT -3′ (SEQ ID NO: 126), a 27-mer oligonucleotide (underlined nucleotides are 2′-MOE modified) (200 μM) is added to the aliquot. Quantification analyses are performed by IEX-HPLC and metabolite identification is carried out by UPLC/MS. 
     In some embodiments, the siRNA duplexes having a 3′ terminal diastereomerically pure phosphorothioate with the Sp configuration show higher metabolic stability in human serum compared to the siRNAs having the phosphorothioate with the Rp configuration, or the stereorandom diastereomixture, at the same position. In other embodiments, the siRNA duplexes having a 5′ terminal diastereomerically pure phosphorothioate with the Sp configuration show higher metabolic stability in human serum compared to the siRNAs having the phosphorothioate with the Rp configuration, or the stereorandom diastereomixture, at the same position. In other embodiments, siRNA duplexes having diastereomerically pure phosphorothioates with the Sp configuration at both the 3′ and the 5′ extremities show higher metabolic stability in human serum compared to the siRNAs having the phosphorothioate with the Rp configuration, or the stereorandom diastereomixtures, at the same positions. In other embodiments, siRNA duplexes having multiple diastereomerically pure phosphorothioates with the Sp configuration show higher metabolic stability in human serum compared to the siRNAs having multiple phosphorothioates with the Rp configuration, or the stereorandom diastereomixtures. In other embodiments, siRNA duplexes having full backbone of diastereomerically pure phosphorothioates with the Sp configuration show higher metabolic stability in human serum compared to the siRNAs having the full backbone of phosphorothioates with the Rp configuration, or the stereorandom diastereomixtures. 
     Example 88. Chirally Controlled Oligonucleotide Compositions Show Different Potency In Vitro as Compared with Chirally Uncontrolled Compositions Having the Same Sequence 
     The present Example compares in vitro pharmacological activity of chirally pure oligonucleotides with that observed for the “parent” stereorandom mixture (i.e., for a composition containing oligonucleotides of the same sequence as the chirally pure oligonucleotides but not displaying chiral purity, for example as a result of having been prepared via a stereorandom process). Four chirally pure oligonucleotides, each of which had a sequence complementary to that of a particular target transcript or gene encoding a protein of interest were synthesized, formulated, and transfected into primary mouse hepatocytes. mRNA levels were quantified to assess level of suppression. In this Example, oligonucleotides having a sequence antisense to (and therefore targeting) human Apolipoprotein-B (ApoB) were used for proof-of-concept in transgenic mice expressing human ApoB. 
     Transfection of Mouse Primary Hepatocytes with Oligonucleotides 
     C57BL6 male mice, 7 weeks of age were used to extract and plate mouse primary hepatocytes in 96-well plates (without overlay) (Celsis/Bioreclamation IVT). Transfection of primary hepatocytes was carried out with lipofectin (Life Technologies, cat. No. 18292-037) using the manufacturer&#39;s protocol, using 0.5 ul of Lipofectin per 96-plate well. Twelve, 1:3 siRNA duplex dilutions were created starting at 6 uM. 10 ul of 10× oligo was then lipoplexed with a prepared mixture of 9.5 ul of In vitro Gro HI Medium (Celsis Cat. Z99009) serum-free medium and 0.5 ul of Lipofectin per well. After a 10-15 minute incubation, 20 ul of lipoplexed oligo was added to primary hepatocytes in 80 ul of In vitro Gro HI Medium to bring the final volume to 100 ul per well. 24 hours after transfection, cells were lysed. 
     Apolipoprotein B mRNA Assay 
     Total mRNA was purified from cell lysates using MagMAX™-96 Total RNA Isolation Kit (Life Technologies, AM1830); 15 ul of cDNA was synthesized with High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Life Technologies, 4374967). Gene expression was evaluated by Real-Time PCR on a Lightcycler 480 (Roche) using a Probes Master Mix (Roche, 04 707 494 001) according to manufacturer&#39;s protocol using the following primers: Mouse apolipoprotein B primers: 
     [Template: (GenBank Accession Number M35186)] 
                            forward primer:           (SEQ ID NO: 127)           CGTGGGCTCCAGCATTCTA                   reverse primer:           (SEQ ID NO: 128)           AGTCATTTCTGCCTTTGCGTC                   PCR probe:           (SEQ ID NO: 129)           FAM-CCAATGGTCGGGCACTGCTCAA-TAMRA            
Mouse GAPDH Primers:
 
                            forward primer:           (SEQ ID NO: 130)           GGCAAATTCAACGGCACAGT                       reverse primer:           (SEQ ID NO: 131)           GGGTCTCGCTCCTGGAAGAT                       PCR probe:           (SEQ ID NO: 132)           5′ JOE-AAGGCCGAGAATGGGAAGCTTGTCATC-TAMRA 3′            
Data Analysis
 
     The delta delta Ct method was used to calculate values. For each sample, the mean ApoB signal was normalized to the mean GAPDH signal, and then normalized to the mean corresponding ratio for mock transfected and untreated samples, to obtain the relative level of ApoB protein expression. The “parent” stereorandom mixture was used for reference. A four-parameter linear regression curve was fitted to the data, and the bottom and top were constrained to a 0% and 100% constants respectively in order to calculate a relative IC50 using Graphpad Prism. 
     The in vitro dose-responses ( FIGS.  71  and  72   ) show that the chirally pure oligonucleotides have significantly different potencies, with ONT-83 being the most potent, and ONT-84 and ONT-85 being the least potent, with a 8.6-fold difference in IC50s (Table E-39). 
     
       
         
           
               
             
               
                 TABLE E-39 
               
             
            
               
                   
               
               
                 IC50 values of Stereoisomers (ONT-83, -84, -85 or -86) for Suppression 
               
               
                 of Mouse Apolipoprotein B/GAPDH mRNA Levels in Primary Mouse Hepatocytes 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Bottom 
                 Top 
                 LogIC50 
                 Hill Slope 
                 IC50 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 ONT-83 
                 0 
                 100 
                 1.6 
                 −0.4 
                 35.8 
               
               
                 ONT-82 
                 0 
                 100 
                 1.8 
                 −0.3 
                 64.4 
               
               
                 ONT-85 
                 0 
                 100 
                 2.5 
                 −0.3 
                 308.0 
               
               
                 ONT-84 
                 0 
                 100 
                 2.5 
                 −0.8 
                 307.8 
               
               
                 ONT-86 
                 0 
                 100 
                 1.7 
                 −0.6 
                 51.2 
               
               
                   
               
            
           
         
       
     
     The results presented in this Example demonstrate, for instance, that chirally pure oligonucleotide compositions can have significantly different pharmacological activity in vitro as compared with an appropriate reference (e.g., a preparation of oligonucleotides of the same sequence but different chiral specificity, including particularly stereorandom preparations), and specifically as compared with a “parental” stereorandom preparation. Those skilled in the art, in light of this demonstration, will appreciate that chirally controlled oligonucleotide compositions provided by the present disclosure have unexpected activities and characteristics. 
     EQUIVALENTS 
     Having described some illustrative embodiments of the invention, it should be apparent to those skilled in the art that the foregoing is merely illustrative and not limiting, having been presented by way of example only. Numerous modifications and other illustrative embodiments are within the scope of one of ordinary skill in the art and are contemplated as falling within the scope of the invention. In particular, although many of the examples presented herein involve specific combinations of method acts or system elements, it should be understood that those acts and those elements may be combined in other ways to accomplish the same objectives. Acts, elements, and features discussed only in connection with one embodiment are not intended to be excluded from a similar role in other embodiments. Further, for the one or more means-plus-function limitations recited in the following claims, the means are not intended to be limited to the means disclosed herein for performing the recited function, but are intended to cover in scope any means, known now or later developed, for performing the recited function. 
     Use of ordinal terms such as “first”, “second”, “third”, etc., in the claims to modify a claim element does not by itself connote any priority, precedence, or order of one claim element over another or the temporal order in which acts of a method are performed, but are used merely as labels to distinguish one claim element having a certain name from another element having a same name (but for use of the ordinal term) to distinguish the claim elements. Similarly, use of a), b), etc., or i), ii), etc. does not by itself connote any priority, precedence, or order of steps in the claims. Similarly, the use of these terms in the specification does not by itself connote any required priority, precedence, or order. 
     The foregoing written specification is considered to be sufficient to enable one skilled in the art to practice the invention. The present invention is not to be limited in scope by examples provided, since the examples are intended as a single illustration of one aspect of the invention and other functionally equivalent embodiments are within the scope of the invention. Various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description and fall within the scope of the appended claims. The advantages and objects of the invention are not necessarily encompassed by each embodiment of the invention. 
     APPENDIX (A) 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                   
                   
                   
                   
                   
                 SEQ 
               
               
                   
                   
                 Gene 
                   
                   
                 ID 
               
               
                 Accession # 
                 Unigene # 
                 Symbol 
                 Name 
                 Sequence 
                 NO: 
               
               
                   
               
             
            
               
                 NM_003467 
                 Hs.421986 
                 CXCR4 
                 Chemokine (C-X-C motif) 
                 5′-UAAAAUCUUCCUGCCCACCdTdT-3′ 
                 139 
               
               
                   
                   
                   
                 receptor 4 
                   
                   
               
               
                   
               
               
                 NM_003467 
                 Hs.421986 
                 CXCR4 
                 Chemokine (C-X-C motif) 
                 5′-GGAAGCUGUUGGCUGAAAAdTdT-3′ 
                 140 
               
               
                   
                   
                   
                 receptor 4 
                   
                   
               
               
                   
               
               
                 NM_006799.2 
                 Hs.72026 
                 PRSS21 
                 Protease, serine, 21 (testisin) 
                 5′-CACAUCCAGCCCAUCUGUC(dTdT)-3′ 
                 141 
               
               
                   
               
               
                 NM_000117.1 
                 Hs.522823 
                 EMD 
                 Emerin 
                 5′-CCGUGCUCCUGGGGCUGGG(dTdT)-3′ 
                 142 
               
               
                   
               
               
                 NM_001350.3 
                 Hs.336916 
                 DAXX 
                 Death-associated protein 6 
                 5′-GGAGUUGGAUCUCUCAGAA(dTdT)-3′ 
                 143 
               
               
                   
               
               
                 NM_003014.2 
                 Hs.105700 
                 SFRP4 
                 Secreted frizzled-related 
                 5′-AAGTCCCGCTCATTACAAATT-3′ 
                 144 
               
               
                   
                   
                   
                 protein 4 
                   
                   
               
               
                   
               
               
                 NM_015062.3 
                 Hs.533551 
                 PPRC1 
                 Peroxisome proliferative 
                 5′-AAGACCAGCCUCUUUGCCCAG-3′ 
                 145 
               
               
                   
                   
                   
                 activated receptor, gamma, 
                   
                   
               
               
                   
                   
                   
                 coactivator-related 1 
                   
                   
               
               
                   
               
               
                 NM_001005360.1 
                 Hs.211463 
                 DNM2 
                 Dynamin 2 
                 5′-GGACCAGGCAGAAAACGAG-3 
                 146 
               
               
                   
               
               
                 NM_001904.2 
                 Hs.476018 
                 CTNNB1 
                 Catenin (cadherin-associated 
                 5′-CUAUCAGGAUGACGCGG-3′ 
                 147 
               
               
                   
                   
                   
                 Protein), beta 1, 88 kDa 
                   
                   
               
               
                   
               
               
                 NM_153831.2 
                 Hs.395482 
                 PTK2 
                 PTK2 protein tyrosine kinase 2 
                 5′-AACCACCUGGGCCAGUAUUAU-dTT-3′ 
                 148 
               
               
                   
               
               
                 NM_001429.2 
                 Hs.517517 
                 EP300 
                 E1A binding protein p300 
                 5′-UGACACAGGCAGGCUUGACUU-3′ 
                 149 
               
               
                   
               
               
                 NM_005904.2 
                 Hs.465087 
                 SMAD7 
                 SMAD, mothers against DPP 
                 5′ AA GCU CAA UUC GGA CAA CAA 
                 150 
               
               
                   
                   
                   
                 homolog 7 (Drosophila) 
                 G 3′ 
                   
               
               
                   
               
               
                 NM_001904.2 
                 Hs.476018 
                 CTNNB1 
                 Catenin (cadherin-associated 
                 5′ AAG UCC UGU AUG AGU GGG 
                 151 
               
               
                   
                   
                   
                 protein), beta 1, 88 kDa 
                 AAC 3′ 
                   
               
               
                   
               
               
                 NM_175847.1 
                 Hs.172550 
                 PTBP1 
                 Polypyrimidine tract binding 
                 5′-TCG ACG AAC ATC TAC AAC GCC 
                 152 
               
               
                   
                   
                   
                 protein 1 
                 TGC TTC AAG AGA GCA GGC GTT 
                   
               
               
                   
                   
                   
                   
                 GTA GAT GTT CTT TTT TT-3′ 
                   
               
               
                   
               
               
                 NM_175847.1 
                 Hs.172550 
                 PTBP1 
                 Polypyrimidine tract binding 
                 5′-TCG ACC AAT GAC AAG AGC CGT 
                 153 
               
               
                   
                   
                   
                 Protein 1 
                 GAC TTC AAG AGA GTC ACG GCT 
                   
               
               
                   
                   
                   
                   
                 CTT GTC ATT GTT TTT TT-3′ 
                   
               
               
                   
               
               
                 NM_002659.2 
                 Hs.466871 
                 PLAUR 
                 Plasminogen activator, 
                 5′-GGTGAAGAAGGGCGTCCAA-3′ 
                 154 
               
               
                   
                   
                   
                 urokinase receptor 
                   
                   
               
               
                   
               
               
                 NM_033360.2 
                 Hs.505033 
                 KRAS2 
                 V-Ki-ras2 Kirsten rat sarcoma 
                 5′GATCCGTTGGAGCTGTTGGCGTAGTTCA 
                 155 
               
               
                   
                   
                   
                 2 viral oncogene homolog 
                 AGAGACTACGCCAACAGCTCCAACTTTTTG 
                   
               
               
                   
                   
                   
                   
                 GAAA-3′ 
                   
               
               
                   
               
               
                 NM_002959.4 
                 Hs.485195 
                 SORT1 
                 Sortilin 1 
                 5′-AGGTGGTGTTAACAGCAGAG-3′ 
                 156 
               
               
                   
               
               
                 NM_002959.4 
                 Hs.485195 
                 SORT1 
                 Sortilin 1 
                 5′-AATGTTCCAATGCCCCACTC-3′ 
                 157 
               
               
                   
               
               
                 NM_000743.2 
                 Hs.89605 
                 CHRNA3 
                 Cholinergic receptor, nicotinic, 
                 5′-AACUGCCAGUGGCCAGGGCCU-3′ 
                 158 
               
               
                   
                   
                   
                 alpha polypeptide 3 
                   
                   
               
               
                   
               
               
                 NM_004859.2 
                 Hs.491351 
                 CLTC 
                 Clathrin, heavy polypeptide 
                 5′-AACCUGCGGUCUGGAGUCAAC-3′ 
                 159 
               
               
                   
                   
                   
                 (Hc) 
                   
                   
               
               
                   
               
               
                 NM_004859.2 
                 Hs.491351 
                 CLTC 
                 Clathrin, heavy polypeptide 
                 5′-UAAUCCAAUUCGAAGACCAAU-3′ 
                 160 
               
               
                   
                   
                   
                 (Hc) 
                   
                   
               
               
                   
               
               
                 NM_000038.3 
                 Hs.158932 
                 APC 
                 Adenomatosis polyposis coli 
                 5′-AGGGGCAGCAACTGATGAAAA-3′ 
                 161 
               
               
                   
               
               
                 NM_004850.3 
                 Hs.58617 
                 ROCK2 
                 Rho-associated, coiled-coil 
                 5′-AAGGCATCGCAGAAGGTTTAT-3′ 
                 162 
               
               
                   
                   
                   
                 containing protein kinase 2 
                   
                   
               
               
                   
               
               
                 NM_001274.2 
                 Hs.24529 
                 CHEK1 
                 CHKl checkpoint homolog ( S.   
                 5′-UCGAAGUACUCAGCGUAAG-3′ 
                 163 
               
               
                   
                   
                   
                   pombe ) 
                   
                   
               
               
                   
               
               
                 NM_007194.3 
                 Hs.291363 
                 CHEK2 
                 CHK2 checkpoint homolog ( S.   
                 5′-GAACCUGAGGACCAAGAAC-3′ 
                 164 
               
               
                   
                   
                   
                   pombe ) 
                   
                   
               
               
                   
               
               
                 NM_001901.1 
                 Hs.410037 
                 CTGF 
                 Connective tissue growth factor 
                 5′-AATGTTCTCTTCCAGGTCAGCCCTGT 
                 165 
               
               
                   
                   
                   
                   
                 CTC-3′ 
                   
               
               
                   
               
               
                 NM_001619.2 
                 Hs.83636 
                 ADRBK1 
                 Adrenergic, beta, receptor 
                 5′-AAGAAGUACGAGAAGCUGGAG-3′ 
                 166 
               
               
                   
                   
                   
                 kinase 1 
                   
                   
               
               
                   
               
               
                 NM_005160.2 
                 Hs.517493 
                 ADRBK2 
                 Adrenergic, beta, receptor 
                 5′-AAGCAAGCUGUAGAACACGUA-3′ 
                 167 
               
               
                   
                   
                   
                 kinase 2 
                   
                   
               
               
                   
               
               
                 NM_005308.2 
                 Hs.524625 
                 GRK5 
                 G protein-coupled receptor 
                 5′-AAGCCGUGCAAAGAACUCUUU-3′ 
                 168 
               
               
                   
                   
                   
                 kinase 5 
                   
                   
               
               
                   
               
               
                 NM_001004106.1 
                 Hs.235116 
                 GRK6 
                 G protein-coupled receptor 
                 5-AACAGUAGGUUUGUAGUGAGC-3′ 
                 169 
               
               
                   
                   
                   
                 kinase 6 
                   
                   
               
               
                   
               
               
                 NM_017556.1 
                 Hs.530101 
                 FBLP-1 
                 Filamin-binding LIM protein-1 
                 5′-AAAGGGGCAUCCACAGACAUC-3′ 
                 170 
               
               
                   
               
               
                 NM_005857.2 
                 Hs.132642 
                 ZMPSTE24 
                 Zinc metallopeptidase (STE24 
                 5′-TTATTCTTCTCTTTGGAGGA-3′ 
                 171 
               
               
                   
                   
                   
                 homolog, yeast) 
                   
                   
               
               
                   
               
               
                 NM_005572 
                 Hs.491359 
                 LMNA 
                 Lamin A/C 
                 5′-ACTGGACTTCCAGAAGAAC-3′ 
                 172 
               
               
                   
               
               
                 NM_015878.3 
                 Hs.459106 
                 OAZIN 
                 Ornithine decarboxylase 
                 5′-AATTGCACGTAATCACCCAAA-3′ 
                 173 
               
               
                   
                   
                   
                 antizyme inhibitor 
                   
                   
               
               
                   
               
               
                 NM_015878.3 
                 Hs.459106 
                 OAZIN 
                 Ornithine decarboxylase 
                 5′-AAGAAATACAAGGAAGATGAG-3′ 
                 174 
               
               
                   
                   
                   
                 antizyme inhibitor 
                   
                   
               
               
                   
               
               
                 NM_001664.2 
                 Hs.247077 
                 RHOA 
                 Ras homolog gene family, 
                 5′-GACAUGCUUGCUCAUAGUCTT-3′ 
                 175 
               
               
                   
                   
                   
                 member A 
                   
                   
               
               
                   
               
               
                 NM_175744.3 
                 Hs.502659 
                 RHOC 
                 Ras homolog gene family, 
                 5′-GACCUGCCUCCUCAUCGUCTT-3′ 
                 176 
               
               
                   
                   
                   
                 member C 
                   
                   
               
               
                   
               
               
                 NM_000041.2 
                 Hs.515465 
                 APOE 
                 Apolipoprotein E 
                 5′-AAGGTGGAGCAAGCGGTGGAG-3′ 
                 177 
               
               
                   
               
               
                 NM_000041.2 
                 Hs.515465 
                 APOE 
                 Apolipoprotein E 
                 5′-AAGGAGTTGAAGGCCTACAAA-3′ 
                 178 
               
               
                   
               
               
                 AF520590.1 
                 Hs.53600 
                 BAK1 
                 BCL2-antagonist/killer 1 
                 5′-UGCCUACGAACUCUUCACCdTdT-3′ 
                 179 
               
               
                   
               
               
                 NM_138761.2 
                 Hs.159428 
                 BAX 
                 BCL2-associated X protein 
                 5′-UAUGGAGCUGCAGAGGAUGdTdT-3′ 
                 180 
               
               
                   
               
               
                 NM_005733.1 
                 Hs.73625 
                 KIF20A 
                 Kinesin family member 20A 
                 5′-TTGGCCAAGCCACACACAG-3′ 
                 181 
               
               
                   
               
               
                 NM_005733.1 
                 Hs.73625 
                 KIF20A 
                 Kinesin family member 20A 
                 5′-GTTCTCAGCCATTGCTAGC-3′ 
                 182 
               
               
                   
               
               
                 NM_005733.1 
                 Hs.73625 
                 KIF20A 
                 Kinesin family member 20A 
                 5′-GGCAGCATGTATTGCTGAG-3′ 
                 183 
               
               
                   
               
               
                 NM_014034.1 
                 Hs.292316 
                 ASF1A 
                 ASF1 anti-silencing function 1 
                 5′-AAUCCAGGACUCAUUCCAGAU-3′ 
                 184 
               
               
                   
                   
                   
                 homolog A ( S. cerevisiae ) 
                   
                   
               
               
                   
               
               
                 NM_014034.1 
                 Hs.292316 
                 ASF1A 
                 ASF1 anti-silencing function 1 
                 5′-AAGUGAAGAAUACGAUCAAGU-3′ 
                 185 
               
               
                   
                   
                   
                 homolog A ( S. cerevisiae ) 
                   
                   
               
               
                   
               
               
                 NM_018154.1 
                 Hs.26516 
                 ASF1B 
                 ASF1 anti-silencing function 1 
                 5′-AACAACGAGUACCUCAACCCU-3′ 
                 186 
               
               
                   
                   
                   
                 homolog B ( S. cerevisiae ) 
                   
                   
               
               
                   
               
               
                 NM_022110.3 
                 Hs.520042 
                 WISp39 
                 FK506 binding protein like 
                 5′ AACGCUUGAGCUGGAAGG 3′ 
                 187 
               
               
                   
               
               
                 NM_022110.3 
                 Hs.520042 
                 WISp39 
                 FK506 binding protein like 
                 5′-CCUUCAAGCUUCUGAUCUC-3′ 
                 188 
               
               
                   
               
               
                 NM_000389.2 
                 Hs.370771 
                 CDKN1A 
                 Cyclin-dependent kinase 
                 5′-AACUUCGACUUUGUCACCGAG-3′ 
                 189 
               
               
                   
                   
                   
                 inhibitor 1A (p21, Cip1) 
                   
                   
               
               
                   
               
               
                 NM_004064.2 
                 Hs.238990 
                 CDKN1B 
                 Cyclin-dependent kinase 
                 5′-AAGCACUGCAGAGACAUGGAAG-3′ 
                 190 
               
               
                   
                   
                   
                 inhibitor 1B (p27, Kip1) 
                   
                   
               
               
                   
               
               
                 NM_033084.2 
                 Hs.208388 
                 FANCD2 
                 Fanconi anemia, 
                 5′-AACAGCCATGGATACACTTGA-3′ 
                 191 
               
               
                   
                   
                   
                 complementation group D2 
                   
                   
               
               
                   
               
               
                 NM_001641.2 
                 Hs.73722 
                 APEX1 
                 APEX nuclease 
                 5′-AATGACAAAGAGGCAGCAGG-3′ 
                 192 
               
               
                   
                   
                   
                 (multifunctional DNA repair  
                   
                   
               
               
                   
                   
                   
                 enzyme) 1 
                   
                   
               
               
                   
               
               
                 NM_001641.2 
                 Hs.73722 
                 APEX1 
                 APEX nuclease 
                 5′-AACCTGCCACACTCAAGATC-3′ 
                 193 
               
               
                   
                   
                   
                 (multifunctional DNA repair 
                   
                   
               
               
                   
                   
                   
                 enzyme) 1 
                   
                   
               
               
                   
               
               
                 NM_001641.2 
                 Hs.73722 
                 APEX1 
                 APEX nuclease 
                 5′-AGCTGAACTTCAGGAGCTGCC-3′ 
                 194 
               
               
                   
                   
                   
                 (multifunctional DNA repair 
                   
                   
               
               
                   
                   
                   
                 enzyme) 1 
                   
                   
               
               
                   
               
               
                 NM_001641.2 
                 Hs.73722 
                 APEX1 
                 APEX nuclease 
                 5′-AAGCCTTTCGCAAGTTCCTGA-3′ 
                 195 
               
               
                   
                   
                   
                 (multifunctional DNA repair 
                   
                   
               
               
                   
                   
                   
                 enzyme) 1 
                   
                   
               
               
                   
               
               
                 NM_001641.2 
                 Hs.73722 
                 APEX1 
                 APEX nuclease 
                 5′-ACGGCATAGGCGATGAGGAG-3′ 
                 196 
               
               
                   
                   
                   
                 (multifunctional DNA repair 
                   
                   
               
               
                   
                   
                   
                 enzyme) 1 
                   
                   
               
               
                   
               
               
                 NM_001641.2 
                 Hs.73722 
                 APEX1 
                 APEX nuclease 
                 5′-AGGAAGGCCGGGTGATTGTG-3′ 
                 197 
               
               
                   
                   
                   
                 (multifunctional DNA repair 
                   
                   
               
               
                   
                   
                   
                 enzyme) 1 
                   
                   
               
               
                   
               
               
                 NM_001641.2 
                 Hs.73722 
                 APEX1 
                 APEX nuclease 
                 5′-GTCTGGTACGACTGGAGTA-3′ 
                 198 
               
               
                   
                   
                   
                 (multifunctional DNA repair 
                   
                   
               
               
                   
                   
                   
                 enzyme) 1 
                   
                   
               
               
                   
               
               
                 NM_001641.2 
                 Hs.73722 
                 APEX1 
                 APEX nuclease 
                 5′-GACAGCTTTAGGCACCTCTA-3′ 
                 199 
               
               
                   
                   
                   
                 (multifunctional DNA repair 
                   
                   
               
               
                   
                   
                   
                 enzyme) 1 
                   
                   
               
               
                   
               
               
                 NM_015641.2 
                 Hs.533391 
                 TES 
                 Testis derived transcript (3 
                 5′-GGAUUCGAACUGCACUUCU-3′ 
                 200 
               
               
                   
                   
                   
                 LIM domains) 
                   
                   
               
               
                   
               
               
                 NM_015641.2 
                 Hs.533391 
                 TES 
                 Testis derived transcript (3 
                 5′-ACUGUGGCACCCAGCUUGU-3′ 
                 201 
               
               
                   
                   
                   
                 LIM domains) 
                   
                   
               
               
                   
               
               
                 NM_003461.3 
                 Hs.490415 
                 ZYX 
                 Zyxin 
                 5′-GCCCAAAGUGAAUCCCUUC-3′ 
                 202 
               
               
                   
               
               
                 NM_002880.2 
                 Hs.159130 
                 RAF1 
                 V-raf-1 murine leukemia viral 
                 5′-TTTGAATATCTGTGCTGAGAACACA 
                 203 
               
               
                   
                   
                   
                 oncogene homolog 1 
                 GTTCTCAGCACAGATATTCTTTTT-3′ 
                   
               
               
                   
               
               
                 NM_002880.2 
                 Hs.159130 
                 RAF1 
                 V-raf-1 murine leukemia viral 
                 5′-TTTGTCAATTAGCTGGAACATCACAG 
                 204 
               
               
                   
                   
                   
                 oncogene homolog 1 
                 ATGTTCCAGCTAATTGACTTTTT-3′ 
                   
               
               
                   
               
               
                 NM_004506.2 
                 Hs.158195 
                 HSF2 
                 Heat shock transcription 
                 5′-AATGAGAAAAGCAAAAGGTGCCCTGT 
                 205 
               
               
                   
                   
                   
                 factor 2 
                 CTC-3′ 
                   
               
               
                   
               
               
                 NM_005356.2 
                 Hs.470627 
                 LCK 
                 Lymphocyte-specific protein 
                 5′-CAUCGAUGUGUGUGAGAACUGC-3′ 
                 206 
               
               
                   
                   
                   
                 tyrosine kinase 
                   
                   
               
               
                   
               
               
                 NM_005546.3 
                 Hs.483938 
                 ITK 
                 IL2-inducible T-cell kinase 
                 5′-CUGUUCUCAGCUGGAGAAGCUU-3′ 
                 207 
               
               
                   
               
               
                 NM_005546.3 
                 Hs.483938 
                 ITK 
                 IL2-inducible T-cell kinase 
                 5′-GGAGCCUUCAUGGUAAGGGAUU-3′ 
                 208 
               
               
                   
               
               
                 NM_002133.1 
                 Hs.517581 
                 HMOX1 
                 Heme oxygenase (decycling) 1 
                 5′-GGCACCATGAAGGCG-3′ 
                 209 
               
               
                   
               
               
                 NM_000639.1 
                 Hs.2007 
                 FASLG 
                 Tumor necrosis factor (ligand) 
                 5′-CUGGGCU GUACU UUGUA UATT-3′ 
                 210 
               
               
                   
                   
                   
                 superfamily, member 6 
                   
                   
               
               
                   
               
               
                 NM_018417.2 
                 Hs.320892 
                 SAC 
                 Testicular soluble adenylyl 
                 5′-AUGUAGCCUGGAGAUCCAUUU-3′ 
                 211 
               
               
                   
                   
                   
                 cyclase 
                   
                   
               
               
                   
               
               
                 NM_003743.3 
                 Hs.412293 
                 NCOA1 
                 Nuclear receptor coactivator 1 
                 5′-CCUCAGGGCAGAGAACCAUCUdTdT-3′ 
                 212 
               
               
                   
               
               
                 NM_005572.2 
                 Hs.491359 
                 LMNA 
                 Lamin A/C 
                 5′-CUGGACUUCCAGAAGAACAUCdTdT-3′ 
                 213 
               
               
                   
               
               
                 NM_176871.2 
                 Hs.521444 
                 PDLIM2 
                 PDZ and LIM domain 2 
                 5′-AAGAUCCGCCAGAGCCCCUCG-3′ 
                 214 
               
               
                   
                   
                   
                 (mystique) 
                   
                   
               
               
                   
               
               
                 NM_014188.2 
                 Hs.30026 
                 HSPC182 
                 HSPC182 protein 
                 5′-AACAGGGACTCACGTGAAGCT-3′ 
                 215 
               
               
                   
               
               
                 NM_014188.2 
                 Hs.30026 
                 HSPC182 
                 HSPC182 protein 
                 5′-AAGACCTGTTTGATCTGATCC-3′ 
                 216 
               
               
                   
               
               
                 AF263744.1 
                 Hs.519346 
                 ERBB2IP 
                 Erbb2 interacting protein 
                 5′-UAGACUGACCCAGCUGGAAdTdT-3′ 
                 217 
               
               
                   
               
               
                 NM_002583.2 
                 Hs.406074 
                 PAWR 
                 PRKC, apoptosis, WT1, 
                 5′-GAUGCAAUUACACAACAGAdTdT-3′ 
                 218 
               
               
                   
                   
                   
                 regulator 
                   
                   
               
               
                   
               
               
                 NM_003766.2 
                 Hs.12272 
                 BECN1 
                 Beclin 1 (coiled-coil, myosin- 
                 5′-CUCAGGAGAGGAGCCAUUU-3′ 
                 219 
               
               
                   
                   
                   
                 like BCL2 interacting protein) 
                   
                   
               
               
                   
               
               
                 NM_003766.2 
                 Hs.12272 
                 BECN1 
                 Beclin 1 (coiled-coil, myosin- 
                 5′-GAUUGAAGACACAGGAGGC-3′ 
                 220 
               
               
                   
                   
                   
                 like BCL2 interacting protein) 
                   
                   
               
               
                   
               
               
                 NM_004849.1 
                 Hs.486063 
                 APG5L 
                 APG5 autophagy 5-like ( S.   
                 5′-GCAACUCUGGAUGGGAUUG-3′ 
                 221 
               
               
                   
                   
                   
                   cerevisiae ) 
                   
                   
               
               
                   
               
               
                 NM_031482.3 
                 Hs.527193 
                 APG10L 
                 APG10 autophagy 10-like ( S.   
                 5′-GGAGUUCAUGAGUGCUAUA-3′ 
                 222 
               
               
                   
                   
                   
                   cerevisiae ) 
                   
                   
               
               
                   
               
               
                 NM_004707.2 
                 Hs.264482 
                 APG12L 
                 APG12 autophagy 12-like ( S.   
                 5′-CAGAGGAACCUGCUGGCGA-3′ 
                 223 
               
               
                   
                   
                   
                   cerevisiae ) 
                   
                   
               
               
                   
               
               
                 NM_002613.2 
                 Hs.459691 
                 PDPK1 
                 3-phosphoinositide dependent 
                 5′-AACTGGCAACCTCCAGAGAAT-3′ 
                 224 
               
               
                   
                   
                   
                 protein kinase-1 
                   
                   
               
               
                   
               
               
                 NM_002613.2 
                 Hs.459691 
                 PDPK1 
                 3-phosphoinositide dependent 
                 5′-AAGAGACCTCGTGGAGAAACT-3′ 
                 225 
               
               
                   
                   
                   
                 protein kinase-1 
                   
                   
               
               
                   
               
               
                 NM_000314.2 
                 Hs.500466 
                 PTEN 
                 Phosphatase and tensin 
                 5′-AACAGTAGAGGAGCCGTCAAA-3′ 
                 226 
               
               
                   
                   
                   
                 homolog (mutated in multiple 
                   
                   
               
               
                   
                   
                   
                 advanced cancers 1) 
                   
                   
               
               
                   
               
               
                 NM_006092.1 
                 Hs.405153 
                 CARD4 
                 Caspase recruitment domain 
                 5′-GGGUGAGACCAUCUUCAUCTT-3′ 
                 227 
               
               
                   
                   
                   
                 family, member 4 
                   
                   
               
               
                   
               
               
                 NM_006092.1 
                 Hs.405153 
                 CARD4 
                 Caspase recruitment domain 
                 5′-GGCCAAAGUCUAUGAAGAUTT-3′ 
                 228 
               
               
                   
                   
                   
                 family, member 4 
                   
                   
               
               
                   
               
               
                 NM_000598.3 
                 Hs.450230 
                 IGFBP3 
                 Insulin-like growth factor 
                 5′-AAUCAUCAUCAAGAAAGGGCA-3′ 
                 229 
               
               
                   
                   
                   
                 binding protein 3 
                   
                   
               
               
                   
               
               
                 NM_006839.1 
                 Hs.148559 
                 IMMT 
                 Inner membrane protein, 
                 5′-AAUUGCUGGAGCUGGCCUUTT-3′ 
                 230 
               
               
                   
                   
                   
                 mitochondrial (mitofilin) 
                   
                   
               
               
                   
               
               
                 NM_016485.3 
                 Hs.431367 
                 C6ORF55 
                 Chromosome 6 open reading 
                 5′-GAATGAAGATCGATAGTAA-3′ 
                 231 
               
               
                   
                   
                   
                 frame 55 
                   
                   
               
               
                   
               
               
                 NM_016485.3 
                 Hs.431367 
                 C6ORF55 
                 Chromosome 6 open reading 
                 5′-GCACAGGTGTAGCAAGTAA-3′ 
                 232 
               
               
                   
                   
                   
                 frame 55 
                   
                   
               
               
                   
               
               
                 NM_016485.3 
                 Hs.431367 
                 C6ORF55 
                 Chromosome 6 open reading 
                 5′-GGAGAATTATGCTTTGAAA-3′ 
                 233 
               
               
                   
                   
                   
                 frame 55 
                   
                   
               
               
                   
               
               
                 NM_016485.3 
                 Hs.431367 
                 C6ORF55 
                 Chromosome 6 open reading 
                 5′-GCAGTGCTTTGCAGTATGA-3′ 
                 234 
               
               
                   
                   
                   
                 frame 55 
                   
                   
               
               
                   
               
               
                 NM_016410.2 
                 Hs.415534 
                 SNF7DC2 
                 SNF7 domain containing 2 
                 5′-CAGAAAGCCTTGCGAGTTT-3′ 
                 235 
               
               
                   
               
               
                 NM_016410.2 
                 Hs.415534 
                 SNF7DC2 
                 SNF7 domain containing 2 
                 5′-GAATTTGGATTGCCACA3A-3′ 
                 236 
               
               
                   
               
               
                 NM_016410.2 
                 Hs.415534 
                 SNF7DC2 
                 SNF7 domain containing 2 
                 5′-GAAGGTGTTCCCACTGATA-3′ 
                 237 
               
               
                   
               
               
                 NM_016410.2 
                 Hs.415534 
                 SNF7DC2 
                 SNF7 domain containing 2 
                 5′-GAGAGGGTCCTGCAAAGAA-3′ 
                 238 
               
               
                   
               
               
                 NM_199185.1 
                 Hs.519452 
                 NPM1 
                 Nucleophosmin (nucleolar 
                 5′-UGAUGAAAAUGAGCACCAG-3′ 
                 239 
               
               
                   
                   
                   
                 phosphoprotein B23, numatrin 
                   
                   
               
               
                   
               
               
                 NM_003118.2 
                 Hs.111779 
                 SPARC 
                 Secreted protein, acidic 
                 5-AAAATCCCTGCCAGAACCACC-3′ 
                 240 
               
               
                   
                   
                   
                 cysteine-rich 
                   
                   
               
               
                   
               
               
                 NM_003118.2 
                 Hs.111779 
                 SPARC 
                 Secreted protein, acidic, 
                 5-AACAAGACCTTCGACTCTTCC-3′ 
                 241 
               
               
                   
                   
                   
                 cysteine-rich 
                   
                   
               
               
                   
               
               
                 NM_003183.3 
                 Hs.404914 
                 ADAM17 
                 A disintegrin and 
                 5′-AAACGAAAGCGAGTACACT-3′ 
                 242 
               
               
                   
                   
                   
                 metalloproteinase domain 17 
                   
                   
               
               
                   
                   
                   
                 (tumor necrosis factor, alpha, 
                   
                   
               
               
                   
                   
                   
                 converting enzyme) 
                   
                   
               
               
                   
               
               
                 NM_012164.2 
                 Hs.494985 
                 FBXW2 
                 F-box and WD-40 domain 
                 5′-AGATGGACTTCTCTGTACAGG-3′ 
                 243 
               
               
                   
                   
                   
                 protein 2 
                   
                   
               
               
                   
               
               
                 NM_012164.2 
                 Hs.494985 
                 FBXW2 
                 F-box and WD-40 domain 
                 5′-GACATTGTCTGTCTCTGAGGA-3′ 
                 244 
               
               
                   
                   
                   
                 protein 2 
                   
                   
               
               
                   
               
               
                 NM_175940.1 
                 Hs.272813 
                 DUOX1 
                 Dual oxidase 1 
                 5′-GGACUUAUCCUGGCUAGAGtt-3′ 
                 245 
               
               
                   
               
               
                 NM_004503.2 
                 Hs.820 
                 HOXC6 
                 Homeo box C6 
                 5′ CCGGAUCUACUCGACUCCCTT 3′ 
                 246 
               
               
                   
               
               
                 NM_004503.2 
                 Hs.820 
                 HOXC6 
                 Homeo box C6 
                 5′ CCUAAUCACACACUCUGLATT 3′ 
                 247 
               
               
                   
               
               
                 NM_004503.2 
                 Hs.820 
                 HOXC6 
                 Homeo box C6 
                 5′ ACUGCAGACAAAACACCUUTT 3′ 
                 248 
               
               
                   
               
               
                 NM_004503.2 
                 Hs.820 
                 HOXC6 
                 Homeo box C6 
                 5′ UCCAACCUCUGGGUCCGUUTT 3′ 
                 249 
               
               
                   
               
               
                 NM_004503.2 
                 Hs.820 
                 H0XC6 
                 Homeo box C6 
                 5′ ACUGUGACCGUUUCUGUGUTT 3′ 
                 250 
               
               
                   
               
               
                 NM_004503.2 
                 Hs.820 
                 HOXC6 
                 Homeo box C6 
                 5′ CUCAGACUCUACAGAUUGCTT 3′ 
                 251 
               
               
                   
               
               
                 NM_182965.1 
                 Hs.68061 
                 SPHK1 
                 Sphingosine kinase 1 
                 5′-GGG CAA GGC CUU GCA GCU C-3′ 
                 252 
               
               
                   
               
               
                 NM_003329.2 
                 Hs.435136 
                 TXN 
                 Thioredoxin 
                 5′-AUGACUGUCAGGAUGUUGCdTT-3′ 
                 253 
               
               
                   
               
               
                 NM_003329.2 
                 Hs.435136 
                 TXN 
                 Thioredoxin 
                 5′-GCAACAUCCUGACAGUCAUdCC-3′ 
                 254 
               
               
                   
               
               
                 NM_203500.1 
                 Hs.465870 
                 KEAP1 
                 Kelch-like ECH-associated 
                 5′-UGAACGGUGCUGUCAUGUAdTdT-3′ 
                 255 
               
               
                   
                   
                   
                 protein 1 
                   
                   
               
               
                   
               
               
                 NM_005239.4 
                 Hs.517296 
                 ETS2 
                 V-ets erythroblastosis virus 
                 5′-GCAGAGGUUCGGCAUGAAUdTdT-3′ 
                 256 
               
               
                   
                   
                   
                 E26 oncogene homolog 2 
                   
                   
               
               
                   
                   
                   
                 (avian) 
                   
                   
               
               
                   
               
               
                 NM_002067.1 
                 Hs.515056 
                 GNA11 
                 Guanine nucleotide binding 
                 5′-AAGATGTTCGTGGACCTG1AC-3′ 
                 257 
               
               
                   
                   
                   
                 protein (G protein), alpha 11 
                   
                   
               
               
                   
                   
                   
                 (Gq class) 
                   
                   
               
               
                   
               
               
                 NM_004827.1 
                 Hs.480218 
                 ABCG2 
                 ATP-binding cassette, sub- 
                 5′-AAGATGATTGTTCGTCCCTGCTAT 
                 258 
               
               
                   
                   
                   
                 family G (WHITE), member 2 
                 AGTGAGTCGTATTA-3′ 
                   
               
               
                   
               
               
                 NM_000610.3 
                 Hs.502328 
                 CD44 
                 CD44 antigen (homing 
                 5′-GAACGAAUCCUGAAGACAUCU-3′ 
                 259 
               
               
                   
                   
                   
                 function and Indian blood 
                   
                   
               
               
                   
                   
                   
                 group system) 
                   
                   
               
               
                   
               
               
                 NM_003489.1 
                 Hs.155017 
                 NRIP1 
                 Nuclear receptor interacting 
                 5′-GAAGGAAGCUUUGCUAGCU-3′ 
                 260 
               
               
                   
                   
                   
                 protein 1 
                   
                   
               
               
                   
               
               
                 NM_004995.2 
                 Hs.2399 
                 MMP14 
                 Matrix metalloproteinase 14 
                 5′-AAGCCTGGCTACAGCAATATGCCTGT 
                 261 
               
               
                   
                   
                   
                   
                 CTC-3′ 
                   
               
               
                   
               
               
                 NM_022045.2 
                 Hs.546363 
                 MTBP 
                 Mdm2, transformed 3T3 cell 
                 5′ GGCUCAUUUGCACUCAAUU 3′ 
                 262 
               
               
                   
                   
                   
                 double minute 2, p53 binding 
                   
                   
               
               
                   
                   
                   
                 protein (mouse) binding 
                   
                   
               
               
                   
                   
                   
                 protein, 104 kDa 
                   
                   
               
               
                   
               
               
                 NM_002392.2 
                 Hs.369849 
                 MDM2 
                 Mdm2, transformed 3T3 cell 
                 5′ GCCACAAAUCUGAUAGUAU 3′ 
                 263 
               
               
                   
                   
                   
                 double minute 2, p53 binding  
                   
                   
               
               
                   
                   
                   
                 protein (mouse) 
                   
                   
               
               
                   
               
               
                 NM_170707.1 
                 Hs.491359 
                 LMNA 
                 Lamin A/C 
                 5′ CUGGACUUCCAGAAGAACA 3′ 
                 264 
               
               
                   
               
               
                 NM_004759.3 
                 Hs.519276 
                 MAPKAPK2 
                 Mitogen-activated protein 
                 5′-UGACCAUCACCGAGUUUAUdTdT-3′ 
                 265 
               
               
                   
                   
                   
                 kinase-activated protein kinase 
                   
                   
               
               
                   
                   
                   
                 2 
                   
                   
               
               
                   
               
               
                 NM_001948.2 
                 Hs.527980 
                 DUT 
                 DUTP pyrophosphatase 
                 5′-GATTATAGGAAATGTTG-3′ 
                 266 
               
               
                   
               
               
                 NM_016022.1 
                 Hs.108408 
                 APH-1A 
                 Likely ortholog of  C. elegans   
                 5′-AAGAAGGCAGATGAGGGGTTA-3′ 
                 267 
               
               
                   
                   
                   
                 anterior pharynx defective 1A 
                   
                   
               
               
                   
               
               
                 NM_031301.2 
                 Hs.511703 
                 PSFL 
                 Anterior pharynx defective 1B- 
                 5′-AACAAAGATGGACCAACACAG-3′ 
                 268 
               
               
                   
                   
                   
                 like 
                   
                   
               
               
                   
               
               
                 BC007496.1 
                 Hs.36915 
                 SMAD3 
                 SMAD, mothers against DPP 
                 5′-GGACGAGGUCUGCGUGAAUdTdT-3′ 
                 269 
               
               
                   
                   
                   
                 homolog 3 (Drosophila) 
                   
                   
               
               
                   
               
               
                 NM_182763.1 
                 Hs.532826 
                 MCL1 
                 Myeloid cell leukemia 
                 5′-AAGAAACGCGGUAAUCGGACU-3′ 
                 270 
               
               
                   
                   
                   
                 sequence 1 (BCL2-related) 
                   
                   
               
               
                   
               
               
                 NM_001022.3 
                 Hs.438429 
                 RPS19 
                 Ribosomal protein S19 
                 5′-GCACAAAGAGCTTGCTCCCttcaagaga 
                 271 
               
               
                   
                   
                   
                   
                 GGGAGCAAGCTCTTTGTGC-3′ 
                   
               
               
                   
               
               
                 NM_001022.3 
                 Hs.438429 
                 RPS19 
                 Ribosomal protein S19 
                 5′-GTCCGGGAAGCTGAAAGTCttcaagaga 
                 272 
               
               
                   
                   
                   
                   
                 GACTTTCAGCTTCCCGGAC-3′ 
                   
               
               
                   
               
               
                 NM_001022.3 
                 Hs.438429 
                 RPS19 
                 Ribosomal protein S19 
                 5′-GAGATCTGGACAGAATCGCttcaagaga 
                 273 
               
               
                   
                   
                   
                   
                 GCGATTCTGTCCAGATCTC-3′ 
                   
               
               
                   
               
               
                 NM_001400.2 
                 Hs.154210 
                 EDG1 
                 Endothelial differentiation, 
                 5′-GGAGAACAGCATTAAACTG-3′ 
                 274 
               
               
                   
                   
                   
                 sphingolipid G-protein-coupled 
                   
                   
               
               
                   
                   
                   
                 receptor, 1 
                   
                   
               
               
                   
               
               
                 NM_001001938.1 
                 Hs.546252 
                 C9orf47 
                 Chromosome 9 open reading 
                 5′-GGTCAACATTCTGATGTCT-3′ 
                 275 
               
               
                   
                   
                   
                 frame 47 
                   
                   
               
               
                   
               
               
                 NM_021972.2 
                 Hs.68061 
                 SPHK1 
                 Sphingosine kinase 1 
                 5′-GGGCAAGGCCTTGCAGCTC-3′ 
                 276 
               
               
                   
               
               
                 NM_016068.1 
                 Hs.423968 
                 TTC11 
                 Tetratricopeptide repeat 
                 5′-GTACAATGATGACATCCGTAA-3′ 
                 277 
               
               
                   
                   
                   
                 domain 11 
                   
                   
               
               
                   
               
               
                 NM_016068.1 
                 Hs.423968 
                 TTC11 
                 Tetratricopeptide repeat 
                 5′-GTACGTCCGCGGGTTGCTGCA-3′ 
                 278 
               
               
                   
                   
                   
                 domain 11 
                   
                   
               
               
                   
               
               
                 NM_153831.2 
                 Hs.395482 
                 PTK2 
                 PTK2 protein tyrosine kinase 2 
                 5′-AAGCAUGUGGCCUGCUAUGGA-3′ 
                 279 
               
               
                   
               
               
                 NM_003749.2 
                 Hs.442344 
                 IRS2 
                 Insulin receptor substrate 2 
                 5′-GATCCCGCCTCAACAACAACAACAACT 
                 280 
               
               
                   
                   
                   
                   
                 TCAAGAGAGTTGTTGTTGTTGTTGAGGTTT 
                   
               
               
                   
                   
                   
                   
                 TTTGGAAA-3′ 
                   
               
               
                   
               
               
                 NM_000691.3 
                 Hs.531682 
                 ALDH3A1 
                 Aldehyde dehydrogenase 3 
                 5′-AAG AAG AGC UUC GAG ACU 
                 281 
               
               
                   
                   
                   
                 family, member A1 
                 UUC-3′ 
                   
               
               
                   
               
               
                 NM_000689.3 
                 Hs.76392 
                 ALDH1A1 
                 Aldehyde dehydrogenase 1 
                 5′-AAC TGG GAG AGT ACG GTT 
                 282 
               
               
                   
                   
                   
                 family, member A1 
                 TCC-3′ 
                   
               
               
                   
               
               
                 NM_000604.2 
                 Hs.549034 
                 FGFR1 
                 Fibroblast growth factor 
                 5′-AAGTCGGACGCAACAGAGAAA-3′ 
                 283 
               
               
                   
                   
                   
                 receptor 1 (fms-related 
                   
                   
               
               
                   
                   
                   
                 tyrosine kinase 2, Pfeiffer 
                   
                   
               
               
                   
                   
                   
                 syndrome) 
                   
                   
               
               
                   
               
               
                 NM_006006.3 
                 Hs.171299 
                 ZBTB16 
                 Zinc finger and BTB domain 
                 5′-GGCCAACCAGAUGCGGCUGUU-3′ 
                 284 
               
               
                   
                   
                   
                 containing 16 
                   
                   
               
               
                   
               
               
                 NM_006006.3 
                 Hs.171299 
                 ZBTB16 
                 Zinc finger and BTB domain 
                 5′-GAUGUUUGACAUCCUCUUCUU-3′ 
                 285 
               
               
                   
                   
                   
                 containing 16 
                   
                   
               
               
                   
               
               
                 NM_004348.1 
                 Hs.122116 
                 RUNX2 
                 Runt-related transcription 
                 5′-GGCUGCAAGCAGUAUUUACUU-3′ 
                 286 
               
               
                   
                   
                   
                 factor 2 
                   
                   
               
               
                   
               
               
                 NM_004348.1 
                 Hs.122116 
                 RUNX2 
                 Runt-related transcription 
                 5′-GGACAGAGUCAGAUUACAGUU-3′ 
                 287 
               
               
                   
                   
                   
                 factor 2 
                   
                   
               
               
                   
               
               
                 NM_014382.2 
                 Hs.546361 
                 ATP2C1 
                 ATPase, Ca++ transporting, 
                 5′-AGCCACTGTGGAAGAAGTATATT-3′ 
                 288 
               
               
                   
                   
                   
                 type 2C, member 1 
                   
                   
               
               
                   
               
               
                 NM_002083.2 
                 Hs.2704 
                 GPX2 
                 Glutathione peroxidase 2 
                 5′-CCCUCUGGUUGGUGAUUCAdTdT-3′ 
                 289 
               
               
                   
                   
                   
                 (gastrointestinal) 
                   
                   
               
               
                   
               
               
                 NM_002083.2 
                 Hs.2704 
                 GPX2 
                 Glutathione peroxidase 2 
                 5′-GGAUGAUGGCACCUUCCUAdTdT-3′ 
                 290 
               
               
                   
                   
                   
                 (gastrointestinal) 
                   
                   
               
               
                   
               
               
                 NM_000942.4 
                 Hs.434937 
                 PP1B 
                 Peptidylprolyl isomerase B 
                 5′-AATTGGAGATGAAGATGTAGG-3′ 
                 291 
               
               
                   
                   
                   
                 (cyclophilin B) 
                   
                   
               
               
                   
               
               
                 NM_003153.3 
                 Hs.524518 
                 STAT6 
                 Signal transducer and activator 
                 5′-CAGUUCCGCCACUUGCCAAdTdT-3′ 
                 292 
               
               
                   
                   
                   
                 of transcription 6,  
                   
                   
               
               
                   
                   
                   
                 interleukin-4 induced 
                   
                   
               
               
                   
               
               
                 NM_003153.3 
                 Hs.524518 
                 STAT6 
                 Signal transducer and activator 
                 5′-AGCCUGGUGGACAUUUAUUdTdT-3′ 
                 293 
               
               
                   
                   
                   
                 of transcription 6,  
                   
                   
               
               
                   
                   
                   
                 interleukin-4 induced 
                   
                   
               
               
                   
               
               
                 NM_003153.3 
                 Hs.524518 
                 STAT6 
                 Signal transducer and activator 
                 5′-GAUGUGUGAAACUCUGAACdTdT-3′ 
                 294 
               
               
                   
                   
                   
                 of transcription 6,   
                   
                   
               
               
                   
                   
                   
                 interleukin-4 induced 
                   
                   
               
               
                   
               
               
                 NM_003153.3 
                 Hs.524518 
                 STAT6 
                 Signal transducer and activator 
                 5′-CAGAUGGGUAAGGAUGGCAdTdT-3′ 
                 295 
               
               
                   
                   
                   
                 of transcription 6,  
                   
                   
               
               
                   
                   
                   
                 interleukin-4 induced 
                   
                   
               
               
                   
               
               
                 NM_002945.2 
                 Hs.461925 
                 RPA1 
                 Replication protein A1, 70 kDa 
                 5′-AAGCACUAUCAUUGCGAAUCC-3′ 
                 296 
               
               
                   
               
               
                 NM_003169.2 
                 Hs.437056 
                 SUPT5H 
                 Suppressor of Ty 5 homolog 
                 5′-AACTGGGCGAGTATTACATGA-3′ 
                 297 
               
               
                   
               
               
                 NM_003318.3 
                 Hs.169840 
                 TTK 
                 TTK protein kinase 
                 5′-TGAACAAAGTGAGAGACAT-3′ 
                 298 
               
               
                   
               
               
                 NM_007194.3 
                 Hs.291363 
                 CHEK2 
                 CHK2 checkpoint homolog 
                 5′-AATGTGTGAATGACAACTACT-3′ 
                 299 
               
               
                   
                   
                   
                 ( S. pombe ) 
                   
                   
               
               
                   
               
               
                 NM_002358.2 
                 Hs.533185 
                 MAD2L1 
                 MAD2 mitotic arrest deficient- 
                 5′-AATACGGACTCACCTTGCTTG-3′ 
                 300 
               
               
                   
                   
                   
                 like 1 (yeast) 
                   
                   
               
               
                   
               
               
                 NM_001401.3 
                 Hs.126667 
                 EDG2 
                 Endothelial differentiation, 
                 5′-r(CCGCCGCUUCCAUUUUUCCU)d 
                 301 
               
               
                   
                   
                   
                 lysophosphatidic acid G- 
                 (TT)-3′ 
                   
               
               
                   
                   
                   
                 protein-coupled receptor, 2 
                   
                   
               
               
                   
               
               
                 NM_001401.3 
                 Hs.126667 
                 EDG2 
                 Endothelial differentiation, 
                 5′-r(AGGAAAAAUGGAAGCGGCGGG) 
                 302 
               
               
                   
                   
                   
                 lysophosphatidic acid G- 
                 d(TT)-3′) 
                   
               
               
                   
                   
                   
                 protein-coupled receptor, 2 
                   
                   
               
               
                   
               
               
                 NM_004448.2 
                 Hs.446352 
                 ERBB2 
                 V-erb-b2 erythroblastic 
                 5′-CCUGGAACUCACCUACCUGdTdT-3′ 
                 303 
               
               
                   
                   
                   
                 leukemia viral oncogene 
                   
                   
               
               
                   
                   
                   
                 homolog 2 
                   
                   
               
               
                   
               
               
                 NM_004448.2 
                 Hs.446352 
                 ERBB2 
                 V-erb-b2 erythroblastic 
                 5′-CUACCUUUCUACGGACGUGdTdT-3′ 
                 304 
               
               
                   
                   
                   
                 leukemia viral oncogene 
                   
                   
               
               
                   
                   
                   
                 homolog 2 
                   
                   
               
               
                   
               
               
                 NM_004448.2 
                 Hs.446352 
                 ERBB2 
                 V-crb-b2 crythroblastic 
                 5′-GAUCCGGAAGUACACGAUGdTdT-3′ 
                 305 
               
               
                   
                   
                   
                 leukemia viral oncogene 
                   
                   
               
               
                   
                   
                   
                 homolog 2 
                   
                   
               
               
                   
               
               
                 NM_014812.1 
                 Hs.533635 
                 KAB 
                 KARP-1-binding protein 
                 5′-GAAGGAAUCCUCCAAGUCA-3′ 
                 306 
               
               
                   
               
               
                 NM_002737.2 
                 Hs.531704 
                 PRKCA 
                 Protein kinase C, alpha 
                 5′-AAGCTCCATGTCACAGTACGA-3′ 
                 307 
               
               
                   
               
               
                 NM_212535.1 
                 Hs.460355 
                 PRKCB1 
                 Protein kinase C, beta 1 
                 5′-AAGCGCTGCGTCATGAATGTT-3′ 
                 308 
               
               
                   
               
               
                 NM_138578.1 
                 Hs.516966 
                 BCL2L1 
                 BCL2-like 1 
                 5′-CTG CCT AAG GCG GAT TTG 
                 309 
               
               
                   
                   
                   
                   
                 AAT-3′ 
                   
               
               
                   
               
               
                 NM_138578.1 
                 Hs.516966 
                 BCL2L1 
                 BCL2-like 1 
                 5′-GGC AGG CGA CGA GTT TGA 
                 310 
               
               
                   
                   
                   
                   
                 ACT-3′ 
                   
               
               
                   
               
               
                 NM_138578.1 
                 Hs.516966 
                 BCL2L1 
                 BCL2-like 1 
                 5′-GTG CGT GGA AAG CGT AGA 
                 311 
               
               
                   
                   
                   
                   
                 CAA-3′ 
                   
               
               
                   
               
               
                 NM_004050.2 
                 Hs.410026 
                 BCL2L2 
                 BCL2-like 2 
                 5′-GGC GGA GTT CAC AGC TCT 
                 312 
               
               
                   
                   
                   
                   
                 ATA-3′ 
                   
               
               
                   
               
               
                 NM_004050.2 
                 Hs.410026 
                 BCL2L2 
                 BCL2-like 2 
                 5′-TG GGC ATA AGT GCT GAT  
                 313 
               
               
                   
                   
                   
                   
                 CTA-3′ 
                   
               
               
                   
               
               
                 NM_004050.2 
                 Hs.410026 
                 BCl2L2 
                 BCL2-like 2 
                 5′-CTC GGT CCT GCG ATT ATT 
                 314 
               
               
                   
                   
                   
                   
                 AAT-3′ 
                   
               
               
                   
               
               
                 NM_003443.1 
                 Hs.433764 
                 ZBTB17 
                 Zinc finger and BTB domain 
                 5′-AAGGCCGAGATCAGCAAAGTTCAAGAG 
                 315 
               
               
                   
                   
                   
                 containing 17 
                 ACTTTGCTGATCTCGGCCTTTTTTTT-3′ 
                   
               
               
                   
               
               
                 NM_003345.3 
                 Hs.302903 
                 UBE2I 
                 Ubiquitin-conjugating enzyme 
                 5′-GGC CAG CCA UCA CAA UCA  
                 316 
               
               
                   
                   
                   
                 E21 (UBC9 homolog, yeast) 
                 ATT-3′ 
                   
               
               
                   
               
               
                 NM_003345.3 
                 Hs.302903 
                 UBE2I 
                 Ubiquitin-conjugating enzyme 
                 5′-GGA ACU UCU AAA UGA ACC  
                 317 
               
               
                   
                   
                   
                 E2I (UBC9 homolog, yeast) 
                 ATT-3′ 
                   
               
               
                   
               
               
                 NM_016166.1 
                 Hs.162458 
                 PIAS1 
                 Protein inhibitor of activated 
                 5′-GGU CCA GUU AAG GUU UUG  
                 318 
               
               
                   
                   
                   
                 STAT, 1 
                 UTT-3′ 
                   
               
               
                   
               
               
                 NM_016166.1 
                 Hs.62458 
                 PIAS1 
                 Protein inhibitor of activated 
                 5′-GGU UAC CUU CCA CCU ACA  
                 319 
               
               
                   
                   
                   
                 STAT, 1 
                 ATT-3′ 
                   
               
               
                   
               
               
                 NM_004068.2 
                 Hs.518460 
                 AP2M1 
                 Adaptor-related protein 
                 5′-AAGUGGAUGCCUUUCGGGUCA-3′ 
                 320 
               
               
                   
                   
                   
                 complex 2, mu 1 subunit 
                   
                   
               
               
                   
               
               
                 NM_004068.2 
                 Hs.518460 
                 AP2M1 
                 Adaptor-related protein 
                 5′-AAGGAGAACAGUUCUUGCGGC-3′ 
                 321 
               
               
                   
                   
                   
                 complex 2, mu 1 subunit 
                   
                   
               
               
                   
               
               
                 NM_004068.2 
                 Hs.518460 
                 AP2M1 
                 Adaptor-related protein 
                 5′-AAGGUCCAGU-CAUUCCAAAUG-3′ 
                 322 
               
               
                   
                   
                   
                 complex 2, mu 1 subunit 
                   
                   
               
               
                   
               
               
                 NM_001278.2 
                 Hs.198998 
                 CHUK 
                 Conserved helix-loop-helix 
                 5′-AGGAAGGACCUGUUGACCUUTT-3′ 
                 323 
               
               
                   
                   
                   
                 ubiquitous kinase 
                   
                   
               
               
                   
               
               
                 NM_001556.1 
                 Hs.413513 
                 IKBKB 
                 Inhibitor of kappa light 
                 5′-UGGUGAGCUUAAUGAAUGATT-3′ 
                 324 
               
               
                   
                   
                   
                 polypeptide gene enhancer in 
                   
                   
               
               
                   
                   
                   
                 B-cells, kinase beta 
                   
                   
               
               
                   
               
               
                 NM_021975.2 
                 Hs.502875 
                 RELA 
                 V-rel reticuloendotheliosis  
                 5′-AGAGGACAUUGAGGUGUAUTT-3′ 
                 325 
               
               
                   
                   
                   
                 viral oncogene homolog A 
                   
                   
               
               
                   
               
               
                 NM_000963.1 
                 Hs.196384 
                 PTGS2 
                 Prostaglandin-endoperoxide 
                 5′-AACTGCTCAACACCGGAATTTTT-3′ 
                 326 
               
               
                   
                   
                   
                 synthase 2 
                   
                   
               
               
                   
               
               
                 NM_005427.1 
                 Hs.192132 
                 TP73 
                 Tumor protein p73 
                 5′-CCAUCCUGUACAACUUCAUGUG-3′ 
                 327 
               
               
                   
               
               
                 NM_005157.2 
                 Hs.431048 
                 ABL1 
                 V-abl Abelson murine 
                 5′-CAAUAAGGAAGAAGCCCUUTT-3′ 
                 328 
               
               
                   
                   
                   
                 leukemia viral oncogene 
                   
                   
               
               
                   
                   
                   
                 homolog 1 
                   
                   
               
               
                   
               
               
                 NM_005157.2 
                 Hs.431048 
                 ABL1 
                 V-abl Abelson murine 
                 5′-TTAUUCCUUCUUCGGGAAGUC-3′ 
                 329 
               
               
                   
                   
                   
                 leukemia viral oncogene 
                   
                   
               
               
                   
                   
                   
                 homolog 1 
                   
                   
               
               
                   
               
               
                 NM_001168.1 
                 Hs.514527 
                 BIRC5 
                 Baculoviral IAP repeat- 
                 5′-GGCUGGCUUCAUCCACUGCTT-3′ 
                 330 
               
               
                   
                   
                   
                 containing 5 (survivin) 
                   
                   
               
               
                   
               
               
                 NM_002940.1 
                 Hs.12013 
                 ABCE1 
                 ATP-binding cassette, sub- 
                 5′-CGAAGATGTTGACCTGGTC-3′ 
                 331 
               
               
                   
                   
                   
                 family E (OABP), member 1 
                   
                   
               
               
                   
               
               
                 NM_002940.1 
                 Hs.12013 
                 ABCE1 
                 ATP-binding cassette, sub- 
                 5′-AGAGTTGTCCTGTAGTTCG-3′ 
                 332 
               
               
                   
                   
                   
                 family E (OABP), member 1 
                   
                   
               
               
                   
               
               
                 NM_004208.2 
                 Hs.424932 
                 PDCD8 
                 Programmed cell death 8 
                 5′-GGAAAUAUGGGAAAGAUCCdTdT 
                 333 
               
               
                   
                   
                   
                 (apoptosis-inducing factor) 
                   
                   
               
               
                   
               
               
                 NM_000115.1 
                 Hs.82002 
                 EDNRB 
                 Endothelin receptor type B 
                 5′-GGAGACUUUCAAAUACAUCTTtt-3′ 
                 334 
               
               
                   
               
               
                 NM_001712.2 
                 Hs.512682 
                 CEACAM1 
                 Carcinoembryonic antigen- 
                 5′-AACCTTCTGGAACCCGCCCAC-3′ 
                 335 
               
               
                   
                   
                   
                 related cell adhesion 
                   
                   
               
               
                   
                   
                   
                 molecule 1 
                   
                   
               
               
                   
               
               
                 NM_001712.2 
                 Hs.512682 
                 CEACAM1 
                 Carcinoembryonic antigen- 
                 5′-AATGTTGCAGAGGGGAAGGAG-3′ 
                 336 
               
               
                   
                   
                   
                 related cell adhesion 
                   
                   
               
               
                   
                   
                   
                 molecule 1 
                   
                   
               
               
                   
               
               
                 NM_033284.1 
                 Hs.436900 
                 TBL1Y 
                 Transducin (beta)-like 1Y- 
                 5′-AAGAGAATGGAGCACATGAAA-3′ 
                 337 
               
               
                   
                   
                   
                 linked 
                   
                   
               
               
                   
               
               
                 NM_033284.1 
                 Hs.436900 
                 TBL1Y 
                 Transducin (beta)-like 1Y- 
                 5′-AAGATGAGCATAACCAGTGAC-3′ 
                 338 
               
               
                   
                   
                   
                 linked 
                   
                   
               
               
                   
               
               
                 NM_024665.3 
                 Hs.438970 
                 TBL1XR1 
                 Transducin (beta)-like 1X- 
                 5′-AAGGCCCTATATTTGCATTAA-3′ 
                 339 
               
               
                   
                   
                   
                 linked receptor 1 
                   
                   
               
               
                   
               
               
                 NM_173174.1 
                 Hs.491322 
                 PTK2B 
                 PTK2B protein tyrosine kinase 
                 5′-GTTGGCTGAGTGCTATGGGCTGA-3′ 
                 340 
               
               
                   
                   
                   
                 2 beta 
                   
                   
               
               
                   
               
               
                 NM_006311.2 
                 Hs.462323 
                 NCOR1 
                 Nuclear receptor 
                 5′-GGGCTTAT0GAGGACCCTATGA-3′ 
                 341 
               
               
                   
                   
                   
                 co-repressor 1 
                   
                   
               
               
                   
               
               
                 NM_002211.2 
                 Hs.429052 
                 ITGB1 
                 Integrin, beta 1 
                 5′-GGAACAGCAGAGAAGCTCATTCAAGAG 
                 342 
               
               
                   
                   
                   
                   
                 ATGAGCTTCTCTGCTGTTCCTTTTT-3′ 
                   
               
               
                   
               
               
                 NM_139176.2 
                 Hs.351118 
                 NALP7 
                 NACHT, leucine rich repeat 
                 5′-CACCGAAGCAGCACGACTTCTTCTTCA 
                 343 
               
               
                   
                   
                   
                 and PYD containing 7 
                 AGAGAGAAGAAGTCGTGCTGCTTC-3′ 
                   
               
               
                   
               
               
                 NM_004422.2 
                 Hs.118640 
                 DVL2 
                 Dishevelled, dsh homolog 2 
                 5′-AGGUUCAGCAGCUCCACGGAdTdT-3′ 
                 344 
               
               
                   
                   
                   
                 (Drosophila) 
                   
                   
               
               
                   
               
               
                 NM_001228.2 
                 Hs.369736 
                 CASP8 
                 Caspase 8, apoptosis-related 
                 5′-gatccccCCTCGGGGATACTGTCTGAt 
                 345 
               
               
                   
                   
                   
                 cysteine protease 
                 tcaagagaTCAGACAGTATCCCCGAGGttt 
                   
               
               
                   
                   
                   
                   
                 ttggaaa-3′ 
                   
               
               
                   
               
               
                 NM_001769.2 
                 Hs.114286 
                 CD9 
                 CD9 antigen (p24) 
                 5′-GAGCATCTTCGAGCAAGAA-3′ 
                 346 
               
               
                   
               
               
                 NM_004357.3 
                 Hs.512857 
                 CD151 
                 CD151 antigen 
                 5′-CATGTGGCACCGTTTGCCT-3′ 
                 347 
               
               
                   
               
               
                 NM_003188.2 
                 Hs.485968 
                 MAP3K7 
                 Mitogen-activated protein 
                 5′-UGGCUUAUCUUACACUGGA-3′ 
                 348 
               
               
                   
                   
                   
                 kinase kinase kinase 7 
                   
                   
               
               
                   
               
               
                 NM_006116.2 
                 Hs.507681 
                 MAP3K7IP1 
                 Mitogen-activated protein 
                 5′-GGCUCAAGUUCAGGAGUGAGAACAA-3′ 
                 349 
               
               
                   
                   
                   
                 kinase kinase kinase 7 
                   
                   
               
               
                   
                   
                   
                 interacting protein 1 
                   
                   
               
               
                   
               
               
                 NM_015093.2 
                 Hs.269775 
                 MAP3K7IP2 
                 Mitogen-activated protein 
                 5′-GGAACGACUUCAAAGAGAACUUGAG-3′ 
                 350 
               
               
                   
                   
                   
                 kinase kinase kinase 7 
                   
                   
               
               
                   
                   
                   
                 interacting protein 2 
                   
                   
               
               
                   
               
               
                 NM_001315.1 
                 Hs.485233 
                 MAPK14 
                 Mitogen-activated protein 
                 5′-GCAUUACAACCAGACAGUUGAUAUU-3′ 
                 351 
               
               
                   
                   
                   
                 kinase 14 
                   
                   
               
               
                   
               
               
                 NM_006502.1 
                 Hs.439153 
                 POLH 
                 Polymerase (DNA directed), 
                 5′-GUG GAG CAG CGG CAA AAU  
                 352 
               
               
                   
                   
                   
                 eta 
                 CTT-3′ 
                   
               
               
                   
               
               
                 NM_006502.1 
                 Hs.439153 
                 POLH 
                 Polymerase (DNA directed), 
                 5′-UCC UCA UUU GAG GAA UAA  
                 353 
               
               
                   
                   
                   
                 eta 
                 ATT-3′ 
                   
               
               
                   
               
               
                 NM_006502.1 
                 Hs.439153 
                 POLH 
                 Polymerase (DNA directed), 
                 5′-GGA AUA AAC CUU GUG CAG  
                 354 
               
               
                   
                   
                   
                 eta 
                 UTT-3′ 
                   
               
               
                   
               
               
                 NM_006502.1 
                 Hs.439153 
                 POLH 
                 Polymerase (DNA directed), 
                 5′-UAA ACC UUG UGC AGU UGU  
                 355 
               
               
                   
                   
                   
                 eta 
                 ATT-3′ 
                   
               
               
                   
               
               
                 NM_006502.1 
                 Hs.439153 
                 POLH 
                 Polymerase (DNA directed), 
                 5′-CCU UGU GCA GUU GUA CAG  
                 356 
               
               
                   
                   
                   
                 eta 
                 UTT-3′ 
                   
               
               
                   
               
               
                 NM_015321.1 
                 Hs.371096 
                 MECT1 
                 Mucoepidermoid carcinoma 
                 5′-CCGGCAACCUCGCGGCCAAUU-3′ 
                 357 
               
               
                   
                   
                   
                 translocated 1 
                   
                   
               
               
                   
               
               
                 NM_181715.1 
                 Hs.406392 
                 TORC2 
                 Transducer of regulated cAMP 
                 5′-CGACUACCAUCUGCACUUAUU-3′ 
                 358 
               
               
                   
                   
                   
                 response element-binding 
                   
                   
               
               
                   
                   
                   
                 protein (CREB) 2 
                   
                   
               
               
                   
               
               
                 NM_001079.3 
                 Hs.234569 
                 ZAP70 
                 Zeta-chain (TCR) associated 
                 5′-AACCGGCTCTCCATTGGCATT-3′ 
                 359 
               
               
                   
                   
                   
                 protein kinase 70 kDa 
                   
                   
               
               
                   
               
               
                 NM_004834.3 
                 Hs.431550 
                 MAP4K4 
                 Mitogen-activated protein 
                 5′-GTGGTTGGAAATGGCACCTTT-3′ 
                 360 
               
               
                   
                   
                   
                 kinase kinase kinase kinase 4 
                   
                   
               
               
                   
               
               
                 NM_006191.1 
                 Hs.524498 
                 PA2G4 
                 Proliferation-associated 2G4, 
                 5′-AAGCGACCAGGAUUAUAUUCU-3′ 
                 361 
               
               
                   
                   
                   
                 38 kDa 
                   
                   
               
               
                   
               
               
                 NM_006191.1 
                 Hs.524498 
                 PA2G4 
                 Proliferation-associated 2G4, 
                 5′-AAGUGAGGUGGAAAGGCGUUU-3′ 
                 362 
               
               
                   
                   
                   
                 38 kDa 
                   
                   
               
               
                   
               
               
                 NM_005940.3 
                 Hs.143751 
                 MMP11 
                 Matrix metalloproteinase 11 
                 5′-TCCCATGTCCACTTCGACTATGATGTCA 
                 363 
               
               
                   
                   
                   
                 (stromelysin 3) 
                 AGAGCATCATAGTCGAAGTGGACATTT-3′ 
                   
               
               
                   
               
               
                 NM_005940.3 
                 Hs.143751 
                 MMP11 
                 Matrix metalloproteinase 11 
                 5′-TCCCAGATCTACTTCTTCCGAGGTCAAG 
                 364 
               
               
                   
                   
                   
                 (stromelysin 3) 
                 AGCCTCGGAAGAAGTAGATCTTT-3′ 
                   
               
               
                   
               
               
                 NM_005940.3 
                 Hs.143751 
                 MMP11 
                 Matrix metalloproteinase 11 
                 5′-TCCCAGGATGCTGATGGCTATGCCTTCA 
                 365 
               
               
                   
                   
                   
                 (stromelysin 3) 
                 AGAGAGGCATAGCCATCAGCATCCTTT-3′ 
                   
               
               
                   
               
               
                 NM_003684.3 
                 Hs.371594 
                 MKNK1 
                 MAP kinase interacting 
                 5′-AATGCCCATCTCTATAGGTTT-3′ 
                 366 
               
               
                   
                   
                   
                 serine/threonine kinase 1 
                   
                   
               
               
                   
               
               
                 NM_003668.2 
                 Hs.413901 
                 MAPKAPK5 
                 Mitogen-activated protein 
                 5′-GGAUAUGCGAAGAAAGAUCTT-3′ 
                 367 
               
               
                   
                   
                   
                 kinase-activated protein 
                   
                   
               
               
                   
                   
                   
                 kinase 5 
                   
                   
               
               
                   
               
               
                 NM_004604.3 
                 Hs.83734 
                 STX4A 
                 Syntaxin 4A (placental) 
                 5′-AAGGAGGAAGCTGATGAGAAC-3′ 
                 368 
               
               
                   
               
               
                 NM_004177.3 
                 Hs.530733 
                 STX3A 
                 Syntaxin 3A 
                 5′-AACGTCCGGAACAAACTGAAG-3′ 
                 369 
               
               
                   
               
               
                 NM_001009567.1 
                 Hs.461247 
                 MRC1L1 
                 Mannose receptor, C type 1- 
                 5′-AAGTGGTACGCAGATTGCACG-3′ 
                 370 
               
               
                   
                   
                   
                 like 1 
                   
                   
               
               
                   
               
               
                 NM_002576.3 
                 Hs.435714 
                 PAK1 
                 P21/Cdc42/Rac1-activated 
                 5′-AAGGAGAAGAAAAAGAAGGAC-3′ 
                 371 
               
               
                   
                   
                   
                 kinase 1 
                   
                   
               
               
                   
               
               
                 NM_001664.2 
                 Hs.247077 
                 RHOA 
                 Ras homolog gene family, 
                 5′-GCAGGTAGAGTTGGCTTTG-3′ 
                 372 
               
               
                   
                   
                   
                 member A 
                   
                   
               
               
                   
               
               
                 NM_175744.3 
                 Hs.502659 
                 RHOC 
                 Ras homolog gene family, 
                 5′-GACTATGATCGACTGCGGC-3′ 
                 373 
               
               
                   
                   
                   
                 member C 
                   
                   
               
               
                   
               
               
                 NM_080491.1 
                 Hs.429434 
                 GAB2 
                 GRB2-associated binding 
                 5′-GTGAGAACGATGAGAAATA-3′ 
                 374 
               
               
                   
                   
                   
                 protein 2 
                   
                   
               
               
                   
               
               
                 NM_080491.1 
                 Hs.429434 
                 GAB2 
                 GRB2-associated binding 
                 5′-GTTGGTGCCTAATCACTTA-3′ 
                 375 
               
               
                   
                   
                   
                 protein 2 
                   
                   
               
               
                   
               
               
                 NM_005225.1 
                 Hs.96055 
                 E2F1 
                 E2F transcription factor 1 
                 5′-GACGTGTCAGGACCTTCGT-3′ 
                 376 
               
               
                   
               
               
                 NM_005225.1 
                 Hs.96055 
                 E2F1 
                 E2F transcription factor 1 
                 5′-CTTAACTGGTGTACATTAA-3′ 
                 377 
               
               
                   
               
               
                 NM_006392.2 
                 Hs.376064 
                 NOL5A 
                 Nucleolar protein 5A (56 kDa 
                 5′-CAAUAUGAUCAUCCAGUCCAUUA-3′ 
                 378 
               
               
                   
                   
                   
                 with KKE/D repeat) 
                   
                   
               
               
                   
               
               
                 NM_015934 
                 Hs.471104 
                 NOP5/NOP58 
                 Nucleolar protein 
                 5′-CAAGCAUGCAGCUUCUACCGUUC-3′ 
                 379 
               
               
                   
                   
                   
                 NOP5/NOP58 
                   
                   
               
               
                   
               
               
                 NM_001436 
                 Hs.299002 
                 FBL 
                 Fibrillarin 
                 5′-CAGUCGAGUUCUCCCACCGCUCU-3′ 
                 380 
               
               
                   
               
               
                 NM_006666 
                 Hs.515846 
                 RUVBL2 
                 RuvB-like 2 ( E. coli ) 
                 5′-GAGACCAUCUACGACCUGGGCAC-3′ 
                 381 
               
               
                   
               
               
                 NM_006666 
                 Hs.515846 
                 RUVBL2 
                 RuvB-like 2 ( E. coli ) 
                 5′-GAGAGUGACAUGGCGCCUGUCCU-3′ 
                 382 
               
               
                   
               
               
                 NM_003707.1 
                 Hs.272822 
                 RUVBL2 
                 RuvB-like 1 ( E. coli ) 
                 5′-AAGGAACCAAACAGUUGAAACUG-3′ 
                 383 
               
               
                   
               
               
                 NM_003707.1 
                 Hs.272822 
                 RUVBL2 
                 RuvB-like 1 ( E. coli ) 
                 5′-GAGUCUUCUAUCGCUCCCAUCGU-3′ 
                 384 
               
               
                   
               
               
                 NM_004741 
                 Hs.523238 
                 NOLC1 
                 Nucleolar and coiled-body 
                 5′-AAAUUGAGGUGGAUUCACGAGUU-3′ 
                 385 
               
               
                   
                   
                   
                 phosphoprotein 1 
                   
                   
               
               
                   
               
               
                 NM_032177 
                 Hs.546453 
                 PHAX 
                 RNA U, small nuclear RNA 
                 5′-UAGUAUCAGCGAGGAACAAAUUA-3′ 
                 386 
               
               
                   
                   
                   
                 export adaptor 
                   
                   
               
               
                   
                   
                   
                 (phosphorylation regulated) 
                   
                   
               
               
                   
               
               
                 NM_032177 
                 Hs.546453 
                 PHAX 
                 RNA U, small nuclear RNA 
                 5′-AAGAGUAUAUAGCACAGGAUUUA-3′ 
                 387 
               
               
                   
                   
                   
                 export adaptor 
                   
                   
               
               
                   
                   
                   
                 (phosphorylation regulated) 
                   
                   
               
               
                   
               
               
                 NM_024831 
                 Hs.335068 
                 NCOA6IP 
                 Nuclear receptor coactivator 6 
                 5′-AAGAUUGCCCUUGCUCGCAAUAA-3′ 
                 388 
               
               
                   
                   
                   
                 interacting protein 
                   
                   
               
               
                   
               
               
                 NM_024831 
                 Hs.335068 
                 NCOA6IP 
                 Nuclear receptor coactivator 6 
                 5′-UAUCACCGUAUGAAAUGGAAACU-3′ 
                 389 
               
               
                   
                   
                   
                 interacting protein 
                   
                   
               
               
                   
               
               
                 NM_022874.1 
                 Hs.202179 
                 SMN2 
                 Survival of motor neuron 1, 
                 5′-AAGUGGAAUGGGUAACUCUUCUU-3′ 
                 390 
               
               
                   
                   
                   
                 telomeric 
                   
                   
               
               
                   
               
               
                 NM_012321.2 
                 Hs.515255 
                 LSM4 
                 LSM4 homolog, U6 small 
                 5′-AACGGCCGUCCCAAAGCUGGCUG-3′ 
                 391 
               
               
                   
                   
                   
                 nuclear RNA associated 
                   
                   
               
               
                   
               
               
                 NM_016200.2 
                 Hs.446179 
                 LSM8 
                 LSM8 homolog, U6 small 
                 5′-AAGAAACAGAUUCUGCGCUUGAU-3′ 
                 392 
               
               
                   
                   
                   
                 nuclear RNA associated 
                   
                   
               
               
                   
               
               
                 NM_003142 
                 Hs.546301 
                 SSB 
                 Sjogren syndrome antigen B 
                 5′-GAAUUAGGUCCACUUCAAUGUCC-3′ 
                 393 
               
               
                   
                   
                   
                 (autoantigen La) 
                   
                   
               
               
                   
               
               
                 NM_003142 
                 Hs.546301 
                 SSB 
                 Sjogren syndrome antigen B 
                 5′-AAGAUUCUUCCAUUAAAUUGCCU-3′ 
                 394 
               
               
                   
                   
                   
                 (autoantigen La) 
                   
                   
               
               
                   
               
               
                 NM_001228 
                 Hs.369736 
                 CASP8 
                 Caspase 8, apoptosis-related 
                 5′-AACTACCAGAAAGGTATACCT-3′ 
                 395 
               
               
                   
                   
                   
                 cysteine protease 
                   
                   
               
               
                   
               
               
                 NM_003842.3 
                 Hs.521456 
                 TNFRSF10B 
                 Tumor necrosis factor receptor 
                 5′-AAGACCCTTGTGCTCGTTGTC-3′ 
                 396 
               
               
                   
                   
                   
                 superfamily, member 10b 
                   
                   
               
               
                   
               
               
                 NM_017672.2 
                 Hs.512894 
                 TRPM7 
                 Transient receptor potential 
                 5′-AAGCAGAGTGACCTGGTAGAT-3′ 
                 397 
               
               
                   
                   
                   
                 cation channel, subfamily M, 
                   
                   
               
               
                   
                   
                   
                 member 7 
                   
                   
               
               
                   
               
               
                 NM_007294.1 
                 Hs.194143 
                 BRCA1 
                 Breast cancer 1, early onset 
                 5′-UCACAGUGUCCUUUAUGUAdTdT-3′ 
                 398 
               
               
                   
               
               
                 NM_033238.1 
                 Hs.526464 
                 PML 
                 Promyelocytic leukemia 
                 5′-AUGGCUUCGACGAGUUCAATT-3′ 
                 399 
               
               
                   
               
               
                 NM_000546.2 
                 Hs.408312 
                 TP53 
                 Tumor protein p53 (Li- 
                 5′-GCAUGAACCGGAGGCCCAUTT-3′ 
                 400 
               
               
                   
                   
                   
                 Fraumeni syndrome) 
                   
                   
               
               
                   
               
               
                 NM_002198.1 
                 Hs.436061 
                 IRF1 
                 Interferon regulatory factor 1 
                 5′-AGACCAGAGCAGGAACAAGTT-3′ 
                 401 
               
               
                   
               
               
                 NM_024790.3 
                 Hs.370147 
                 FLJ22490 
                 Hypothetical protein FLJ22490 
                 5′-GAAGATTTGCGCAGTGGAC-3′ 
                 402 
               
               
                   
               
               
                 NM_000546.2 
                 Hs.408312 
                 TP53 
                 Tumor protein P53 (Li- 
                 5′-UGGUUCACUGAAGACCCAGUU-3′ 
                 403 
               
               
                   
                   
                   
                 Fraumeni syndrome) 
                   
                   
               
               
                   
               
               
                 NM_002880.2 
                 Hs.159130 
                 RAF1 
                 V-raf-1 murine leukemia viral 
                 5′-AUUCCUGCUCAAUGGAUUUdTdT-3′ 
                 404 
               
               
                   
                   
                   
                 oncogene homolog 1 
                   
                   
               
               
                   
               
               
                 NM_198400.1 
                 Hs.1565 
                 NEDD4 
                 Neural precursor cell 
                 5′-TAGAGCCTGGCTGGGTTGTTTTG-3′ 
                 405 
               
               
                   
                   
                   
                 expressed, developmentally 
                   
                   
               
               
                   
                   
                   
                 down-regulated 4 
                   
                   
               
               
                   
               
               
                 NM_015277.2 
                 Hs.185677 
                 NEDD4L 
                 Neural precursor cell 
                 5′-AACCACAACACAAAGTCACAG-3′ 
                 406 
               
               
                   
                   
                   
                 expressed, developmentally 
                   
                   
               
               
                   
                   
                   
                 down-regulated 4-like 
                   
                   
               
               
                   
               
               
                 NM_016931.2 
                 Hs.371036 
                 NOX4 
                 NADPH oxidase 4 
                 5′-AAACCGGCAGGAGUUUACCCAG-3′ 
                 407 
               
               
                   
               
               
                 NM_005975.2 
                 Hs.51133 
                 PTK6 
                 PTK6 protein tyrosine kinase 6 
                 5′-AAGGUGGCCAUUAAGGUGAUU-3′ 
                 408 
               
               
                   
               
               
                 NM_005531.1 
                 Hs.380250 
                 IFI16 
                 Interferon, gamma-inducible 
                 5′-UCAGAAGACCACAAUCUAC-3′ 
                 409 
               
               
                   
                   
                   
                 protein 16 
                   
                   
               
               
                   
               
               
                 NM_000633.1 
                 Hs.150749 
                 BCL2 
                 B-cell CLL/lymphoma 2 
                 5′-GUGAAGUCAACAUGCCUGC-dTdT-3′ 
                 410 
               
               
                   
               
               
                 NM_182981.1 
                 Hs.528383 
                 OKL38 
                 Pregnancy-induced growth 
                 5′-CACCCUACACGAAGCCAGATT-3′ 
                 411 
               
               
                   
                   
                   
                 inhibitor 
                   
                   
               
               
                   
               
               
                 NM_002961.2 
                 Hs.81256 
                 S100A4 
                 S100 calcium binding protein 
                 5′-GGA CAG AUG AAG CUG CUU  
                 412 
               
               
                   
                   
                   
                 A4 
                 UTT-3′ 
                   
               
               
                   
               
               
                 NM_014585.3 
                 Hs.529285 
                 SLC40A1 
                 Solute carrier family 40  
                 5′-GGTGGACAAGAATGCTAGAC-3′ 
                 413 
               
               
                   
                   
                   
                 (iron-regulated transporter), 
                   
                   
               
               
                   
                   
                   
                 member 1 
                   
                   
               
               
                   
               
               
                 NM_014585.3 
                 Hs.529285 
                 SLC40A1 
                 Solute carrier family 40  
                 5′-GAAGGATTGACCAGTTAACC-3′ 
                 414 
               
               
                   
                   
                   
                 (iron-regulated transporter), 
                   
                   
               
               
                   
                   
                   
                 member 1 
                   
                   
               
               
                   
               
               
                 NM_014585.3 
                 Hs.529285 
                 SLC40A1 
                 Solute carrier family 40  
                 5′-GCTTGAACATGAGCAAGAGC-3′ 
                 415 
               
               
                   
                   
                   
                 (iron-regulated transporter), 
                   
                   
               
               
                   
                   
                   
                 member 1 
                   
                   
               
               
                   
               
               
                 NM_021127.1 
                 Hs.96 
                 PMAIP1 
                 Phorbol-12-myristate-13- 
                 5′-AACTTCCGGCAGAAACTTCTG-3′ 
                 416 
               
               
                   
                   
                   
                 acetate-induced protein 1 
                   
                   
               
               
                   
               
               
                 NM_002467.2 
                 Hs.202453 
                 MYC 
                 V-myc myelocytomatosis viral 
                 5′-GCCACAGCAUACAUCCUGUdTdT-3′ 
                 417 
               
               
                   
                   
                   
                 oncogene homolog (avian) 
                   
                   
               
               
                   
               
               
                 NM_002187.2 
                 Hs.674 
                 IL12B 
                 Interleukin 12B 
                 5′-CGCACGCUAAUGCUGGCAU1TdT-3′ 
                 418 
               
               
                   
               
               
                 NM_019887.3 
                 Hs.169611 
                 DIABLO 
                 Diablo homolog (Drosophila) 
                 5′-AAGCGGUGUUUCUCAGAATTGtt-3′ 
                 419 
               
               
                   
               
               
                 NM_017563 
                 Hs.150725 
                 IL17RD 
                 Interleukin 17 receptor D 
                 5′-GUCGG AGGGA AGACA GUGCT T-3′ 
                 420 
               
               
                   
               
               
                 NM_017563 
                 Hs.150725 
                 IL17RD 
                 Interleukin 17 receptor D 
                 5′-GCAUG UGAUU GCUGA CGCCT T-3′ 
                 421 
               
               
                   
               
               
                 NM_003142.2 
                 Hs.546301 
                 SSB 
                 Sjogren syndrome antigen B 
                 5Õ-AAGGCTTCCC AACTGATGC AA-3Õ 
                 422 
               
               
                   
                   
                   
                 (autoantigen La) 
                   
                   
               
               
                   
               
               
                 NM_003142.2 
                 Hs.546301 
                 SSB 
                 Sjogren syndrome antigen B 
                 5Õ-AAGCCAAG GAAGCAT TGGGTA-3Õ 
                 423 
               
               
                   
                   
                   
                 (autoantigen La) 
                   
                   
               
               
                   
               
               
                 NM_003142.2 
                 Hs.546301 
                 SSB 
                 Sjogren syndrome antigen B 
                 5Õ-AAGTACTAGAA GGAGAGGTGG-3Õ 
                 424 
               
               
                   
                   
                   
                 (autoantigen La) 
                   
                   
               
               
                   
               
               
                 NM_006101 
                 Hs.414407 
                 KNTC2 
                 Kinctochore associated 2 
                 5′-GTTCAAAAGCTGGATGATCTT-3′ 
                 425 
               
               
                   
               
               
                 NM_145697 
                 Hs.234545 
                 CDCA1 
                 Cell division cycle associated 1 
                 5′-AAGATACGGTCCAGAAGCTTA-3′ 
                 426 
               
               
                   
               
               
                 NM_003550 
                 Hs.209128 
                 MAD1L1 
                 MAD1 mitotic arrest deficient- 
                 5′-CCAGCGGCTCAAGGAGGTTTT-3′ 
                 427 
               
               
                   
                   
                   
                 like 1 
                   
                   
               
               
                   
               
               
                 NM_002358 
                 Hs.533185 
                 MAD2L1 
                 MAD2 mitotic arrest deficient- 
                 5′-GAGTCGGGACCACAGTTTATT-3′ 
                 428 
               
               
                   
                   
                   
                 like 1 
                   
                   
               
               
                   
               
               
                 NM_004336 
                 Hs.469649 
                 BUB1 
                 BUB1 budding uninhibited by 
                 5′-TAGGCTAATTGTACTGCTCTT-3′ 
                 429 
               
               
                   
                   
                   
                 benzimidazoles 1 homolog 
                   
                   
               
               
                   
               
               
                 NM_001211.4 
                 Hs.36708 
                 BUB1B 
                 BUB1 budding uninhibited by 
                 5′-GGAGATCCTCTACAAAGGGTT-3′ 
                 430 
               
               
                   
                   
                   
                 benzimidazoles 1 homolog beta 
                   
                   
               
               
                   
               
               
                 NM_016343.3 
                 Hs.497741 
                 CENPF 
                 Centromere protein F, 
                 5′-AAGAGATGCTAATAGCAGTTT-3′ 
                 431 
               
               
                   
                   
                   
                 350/400 ka (mitosin) 
                   
                   
               
               
                   
               
               
                 NM_001813 
                 Hs.75573 
                 CENPE 
                 Centromere protein E, 312 kDa 
                 5′-ACTCTTACTGCTCTCCAGTTT-3′ 
                 432 
               
               
                   
               
               
                 NM_004217 
                 Hs.442658 
                 AURKB 
                 Aurora kinase B 
                 5′-CGAGACCTATCGCCGCATCGT-3′ 
                 433 
               
               
                   
               
               
                 NM_005030 
                 Hs.329989 
                 PLK1 
                 Polo-like kinase 1 
                 5′-GGGCGGCTTTGCCAAGTGCTT-3′ 
                 434 
               
               
                   
               
               
                 NM_004104 
                 Hs.83190 
                 FASN 
                 Fatty acid synthase 
                 5′-CCCUGAGAUCCCAGCGCUGdTdT-3′ 
                 435 
               
               
                   
               
               
                 NM_021975.2 
                 Hs.502875 
                 RELA 
                 V-rel reticuloendotheliosis 
                 5′-GATCAATGGCTACACAGGA-3′ 
                 436 
               
               
                   
                   
                   
                 viral oncogene homolog A 
                   
                   
               
               
                   
               
               
                 NM_033256 
                 Hs.348037 
                 PPP1R14A 
                 Protein phosphatase 1, 
                 5′-ACCUGUCGAGGACUUCAUCdTdT-3′ 
                 437 
               
               
                   
                   
                   
                 regulatory (inhibitor) subunit 
                   
                   
               
               
                   
                   
                   
                 14A 
                   
                   
               
               
                   
               
               
                 NM_177966.3 
                 Hs.151293 
                 2′-PDE 
                 2′-phosphodiesterase 
                 5′-GUACAAGGUGGAGCGCAACdTdT-3′ 
                 438 
               
               
                   
               
               
                 NM_015355 
                 Hs.462732 
                 SUZ12 
                 Suppressor of zeste 12 
                 5′-CCCGGAAATTTCCCGTCCC-3′ 
                 439 
               
               
                   
                   
                   
                 homolog 
                   
                   
               
               
                   
               
               
                 NM_015355 
                 Hs.462732 
                 SUZ12 
                 Suppressor of zeste 12 
                 5′-GAGATGACCTGCATTGCCC-3′ 
                 440 
               
               
                   
                   
                   
                 homolog 
                   
                   
               
               
                   
               
               
                 NM_016179.1 
                 Hs.262960 
                 TRPC4 
                 Transient receptor potential 
                 5′-ACUCUUGGUUCAGAAAGGATT-3′ 
                 441 
               
               
                   
                   
                   
                 cation channel, subfamily C,  
                   
                   
               
               
                   
                   
                   
                 member 4 
                   
                   
               
               
                   
               
               
                 NM_000249 
                 Hs.195364 
                 MLH1 
                 MutL homolog 1, colon cancer, 
                 5′-GGTTCACTACTAGTAAACT-3′ 
                 442 
               
               
                   
                   
                   
                 nonpolyposis type 2 
                   
                   
               
               
                   
               
               
                 NM_000534 
                 Hs.111749 
                 PMS1 
                 PMS1 postmeiotic segregation 
                 5′-GGAATCTACTCGTTTGTAT-3′ 
                 443 
               
               
                   
                   
                   
                 increased 1 
                   
                   
               
               
                   
               
               
                 NM_002198 
                 Hs.436061 
                 IRF1 
                 Interferon regulatory factor 1 
                 5′-CCAAGAACCAGAGAAAAGATT-3′ 
                 444 
               
               
                   
               
               
                 NM_002199.2 
                 Hs.374097 
                 IRF2 
                 Interferon regulatory factor 2 
                 5′-CUCUUUAGAAACUGGGCAATT-3′ 
                 445 
               
               
                   
               
               
                 NM_000546.2 
                 Hs.408312 
                 TP53 
                 Tumor protein p53 (Li- 
                 5′-AAGACTCCAGTGGTAATCTAC-3′ 
                 446 
               
               
                   
                   
                   
                 Fraumeni syndrome) 
                   
                   
               
               
                   
               
               
                 NM_000051 
                 Hs.435561 
                 ATM 
                 Ataxia telangiectasia mutated 
                 5′-TAGAGCTACAGAACGAAAG-3′ 
                 447 
               
               
                   
                   
                   
                 (includes complementation 
                   
                   
               
               
                   
                   
                   
                 groups A, C and D) 
                   
                   
               
               
                   
               
               
                 NM_000051 
                 Hs.435561 
                 ATM 
                 Ataxia telangiectasia mutated 
                 5′-GAATGTGAACACCACCAAA-3′ 
                 448 
               
               
                   
                   
                   
                 (includes complementation 
                   
                   
               
               
                   
                   
                   
                 groups A, C and D) 
                   
                   
               
               
                   
               
               
                 NM_000051 
                 Hs.435561 
                 ATM 
                 Ataxia telangiectasia mutated 
                 5′-CTACACAAATATTGAGGAT-3′ 
                 449 
               
               
                   
                   
                   
                 (includes complementation 
                   
                   
               
               
                   
                   
                   
                 groups A, C and D) 
                   
                   
               
               
                   
               
               
                 NM_000051 
                 Hs.435561 
                 ATM 
                 Ataxia telangiectasia mutated 
                 5′-CTGTACTTCCATACTTGAT-3′ 
                 450 
               
               
                   
                   
                   
                 (includes complementation 
                   
                   
               
               
                   
                   
                   
                 groups A, C and D) 
                   
                   
               
               
                   
               
               
                 NM_001184 
                 Hs.271791 
                 ATR 
                 Ataxia telangiectasia and Rad3 
                 5′-AAGCCAAGACAAATTCTGTGT-3′ 
                 451 
               
               
                   
                   
                   
                 related 
                   
                   
               
               
                   
               
               
                 NM_001184 
                 Hs.271791 
                 ATR 
                 Ataxia telangiectasia and Rad3 
                 5′-AACCTCCGTGATGTTGCTTGA-3′ 
                 452 
               
               
                   
                   
                   
                 related 
                   
                   
               
               
                   
               
               
                 NM_001798.2 
                 Hs.19192 
                 CDK2 
                 Cyclin-dependent kinase 2 
                 5′-CAAAGCCAGAAACAAGTTG-3′ 
                 453 
               
               
                   
               
               
                 NM_001798.2 
                 Hs.19192 
                 CDK2 
                 Cyclin-dependent kinase 2 
                 5′-AAATAAACTCTACCTGGTT-3′ 
                 454 
               
               
                   
               
               
                 NM_001798.2 
                 Hs.19192 
                 CDK2 
                 Cyclin-dependent kinase 2 
                 5′-AAACCTCAGAATCTGCTTA-3′ 
                 455 
               
               
                   
               
               
                 NM_001798.2 
                 Hs.19192 
                 CDK2 
                 Cyclin-dependent kinase 2 
                 5′-GTTACTTCTATGCCTGATT-3′ 
                 456 
               
               
                   
               
               
                 NM_207003 
                 Hs.469658 
                 BCL2L11 
                 BCL2-like 11 (apoptosis 
                 5′-(GACCGAGAAGGUAGACAAUUG) 
                 457 
               
               
                   
                   
                   
                 facilitator) 
                 d(TT)-3′ 
                   
               
               
                   
               
               
                 NM_000166 
                 Hs.333303 
                 GJB1 
                 Gap junction protein, beta 1, 
                 5′-AAGAGGCACAAGGTCCACATCdTdT-3′ 
                 458 
               
               
                   
                   
                   
                 32 kDa 
                   
                   
               
               
                   
               
               
                 NM_000359 
                 Hs.508950 
                 TGM1 
                 Transglutaminase 1 
                 5′-AUGCAGCUGGAGAUGGCACdTdT-3′ 
                 459 
               
               
                   
               
               
                 NM_024596 
                 Hs.550532 
                 MCPH1 
                 Microcephaly, primary 
                 5′-AGGAAGUUGGAAGGAUCCAdTdT-3′ 
                 460 
               
               
                   
                   
                   
                 autosomal recessive 1 
                   
                   
               
               
                   
               
               
                 NM_024596 
                 Hs.550532 
                 MCPH1 
                 Microcephaly, primary 
                 5′-GAACACUUAUCAAGCCUAAUU-3′ 
                 461 
               
               
                   
                   
                   
                 autosomal recessive 1 
                   
                   
               
               
                   
               
               
                 NM_024596 
                 Hs.550532 
                 MCPH1 
                 Microcephaly, primary 
                 5′-GGAGAGAACAAGCAUAUUUUU-3′ 
                 462 
               
               
                   
                   
                   
                 autosomal recessive 1 
                   
                   
               
               
                   
               
               
                 NM_024596 
                 Hs.550532 
                 MCPH1 
                 Microcephaly, primary 
                 5′-UGAUGUACCUAUUCUCUUAUU-3′ 
                 463 
               
               
                   
                   
                   
                 autosomal recessive 1 
                   
                   
               
               
                   
               
               
                 NM_024596 
                 Hs.550532 
                 MCPH1 
                 Microcephaly, primary 
                 5′-GAUAAGAGAUUUCAGAAGAUU-3′ 
                 464 
               
               
                   
                   
                   
                 autosomal recessive 1 
                   
                   
               
               
                   
               
               
                 NM_024596 
                 Hs.550532 
                 MCPH1 
                 Microcephaly, primary 
                 5′-GUCACCACAGCGCAATGGAdTdT-3′ 
                 465 
               
               
                   
                   
                   
                 autosomal recessive 1 
                   
                   
               
               
                   
               
               
                 NM_000245 
                 Hs.132966 
                 MET 
                 Met proto-oncogene 
                 5′-ACUCUAGAUGCUCAGACUUTT-3′ 
                 466 
               
               
                   
                   
                   
                 (hepatocyte growth factor 
                   
                   
               
               
                   
                   
                   
                 receptor) 
                   
                   
               
               
                   
               
               
                 NM_205860.1 
                 Hs.33446 
                 NR5A2 
                 Nuclear receptor subfamily 5, 
                 5′-AGGATCCATCTTCCTGGTTAC-3′ 
                 467 
               
               
                   
                   
                   
                 group A, member 2 
                   
                   
               
               
                   
               
               
                 NM_182763.1 
                 Hs.532826 
                 MCL1 
                 Myeloid cell leukemia 
                 5′-UAACACCAGTACGGACGGGdTdT-3′ 
                 468 
               
               
                   
                   
                   
                 sequence 1 (BCL2-related) 
                   
                   
               
               
                   
               
               
                 NM_008765 
                 Hs.444870 
                 ORC2L 
                 Origin recognition complex, 
                 5′-UGCUCCUCUCAUGUGGGAU-3′ 
                 469 
               
               
                   
                   
                   
                 subunit 2-like 
                   
                   
               
               
                   
               
               
                 NM_006190 
                 Hs.444870 
                 ORC2L 
                 Origin recognition complex, 
                 5′-UCAUUGGUCAGUUGUCAUC-3′ 
                 470 
               
               
                   
                   
                   
                 subunit 2-like 
                   
                   
               
               
                   
               
               
                 NM_181837 
                 Hs.410228 
                 ORC3L 
                 Origin recognition complex, 
                 5′-GAGACUUGGGCGGUCAAAU-3′ 
                 471 
               
               
                   
                   
                   
                 subunit 3-like 
                   
                   
               
               
                   
               
               
                 NM_002592.2 
                 Hs.147433 
                 PCNA 
                 Proliferating cell nuclear 
                 5′-CGGUGACACUCAGUAUGUC-3′ 
                 472 
               
               
                   
                   
                   
                 antigen 
                   
                   
               
               
                   
               
               
                 NM_016526 
                 Hs.414418 
                 BET1L 
                 Blocked early in transport 1 
                 5′-AAGCAUGACCAGCCUGCUUAC-3′ 
                 473 
               
               
                   
                   
                   
                 homolog ( S. cerevisiae ) like 
                   
                   
               
               
                   
               
               
                 NM_001569 
                 Hs.522819 
                 IRAK1 
                 Interleukin-1 receptor- 
                 5′-GGUUGUCCUUGAGUAAUAAtt-3′ 
                 474 
               
               
                   
                   
                   
                 associated kinase 1 
                   
                   
               
               
                   
               
               
                 NM_080649 
                 Hs.73722 
                 APEX1 
                 APEX nuclease 
                 5′-GUCUGGUACGACUGGAGUACC-3′ 
                 475 
               
               
                   
                   
                   
                 (multifunctional DNA repair 
                   
                   
               
               
                   
                   
                   
                 enzyme) 1 
                   
                   
               
               
                   
               
               
                 NM_002658 
                 Hs.77274 
                 PLAU 
                 Plasminogen activator, 
                 5′-AACATTCACTGGTGCAACTGC-3′ 
                 476 
               
               
                   
                   
                   
                 urokinase 
                   
                   
               
               
                   
               
               
                 NM_001654 
                 Hs.446641 
                 ARAF 
                 V-raf murine sarcoma 3611 
                 5′-AACAACATCTTCCTACATGAG-3′ 
                 477 
               
               
                   
                   
                   
                 viral oncogene homolog 
                   
                   
               
               
                   
               
               
                 NM_004333 
                 Hs.490366 
                 BRAF 
                 V-raf murine sarcoma viral 
                 5′-AAAGAATTGGATCTGGATCAT-3′ 
                 478 
               
               
                   
                   
                   
                 oncogene homolog B1 
                   
                   
               
               
                   
               
               
                 NM_002880 
                 Hs.159130 
                 RAF1 
                 V-raf-1 murine leukemia viral 
                 5′-AAUAGUUCAGCAGUUUGGCUA-3′ 
                 479 
               
               
                   
                   
                   
                 oncogene homolog 1 
                   
                   
               
               
                   
               
               
                 NM_014314 
                 Hs.190622 
                 DDX58 
                 DEAD (Asp-Glu-Ala-Asp) box 
                 5′-GAATTTAAAACCAGAATTATC-3′ 
                 480 
               
               
                   
                   
                   
                 Polypeptide 58 
                   
                   
               
               
                   
               
               
                 NM_000927.3 
                 Hs.489033 
                 ABCB1 
                 ATP-binding cassette, sub- 
                 5′-AAGCGAAGCAGTGGTTCAGGT-3′ 
                 481 
               
               
                   
                   
                   
                 family B (MDR/TAP), member 1 
                   
                   
               
               
                   
               
               
                 NM_001753.3 
                 Hs.74034 
                 CAV1 
                 Caveolin 1, caveolae protein, 
                 5′-AGACGAGCUGAGCGAGAAGCA-3′ 
                 482 
               
               
                   
                   
                   
                 22 kDa 
                   
                   
               
               
                   
               
               
                 NM_001753.3 
                 Hs.74034 
                 CAV1 
                 Caveolin 1, caveolae protein, 
                 5′-CAUCUACAAGCCCAACAACTT-3′ 
                 483 
               
               
                   
                   
                   
                 22 kDa 
                   
                   
               
               
                   
               
               
                 NM_000389.2 
                 Hs.370771 
                 CDKN1A 
                 Cyclin-dependent kinase 
                 5′-CUUCGACUUUGUCACCGAG-3′ 
                 484 
               
               
                   
                   
                   
                 inhibitor 1A (p21, Cip1) 
                   
                   
               
               
                   
               
               
                 NM_007294.1 
                 Hs.194143 
                 BRCA1 
                 Breast cancer 1, early onset 
                 5′-AACCTGTCTCCACAAAGTGTG-3′ 
                 485 
               
               
                   
               
               
                 NM_002105 
                 Hs.477879 
                 H2AFX 
                 H2A histone family, member X 
                 5′-CAA CAA GAA GAC GCG AAU  
                 486 
               
               
                   
                   
                   
                   
                 CdTdT-3′ 
                   
               
               
                   
               
               
                 NM_020382 
                 Hs.443735 
                 SET8 
                 PR/SET domain containing 
                 5′-AAUCGCCUAGGAAGACUGAUC-3′ 
                 487 
               
               
                   
                   
                   
                 protein 8 
                   
                   
               
               
                   
               
               
                 NM_012331 
                 Hs.490981 
                 MSRA 
                 Methionine sulfoxidc reductase A 
                 5′-CCCCUGUAGCGGCCAAACAUU-3′ 
                 488 
               
               
                   
               
               
                 NM_012331 
                 Hs.490981 
                 MSRA 
                 Methionine sulfoxide reductase A 
                 5′-CAAAGUACAAAGGAAUUUAUU-3′ 
                 489 
               
               
                   
               
               
                 NM_012331 
                 Hs.490981 
                 MSRA 
                 Methionine sulfoxide reductase A 
                 5′-CGGGAGGGACAGACUUUCUUU-3′ 
                 490 
               
               
                   
               
               
                 NM_014554 
                 Hs.371957 
                 SENP1 
                 SUMO1/sentrin specific 
                 5′-GTGAACCACAACTCCGTATTC-3′ 
                 491 
               
               
                   
                   
                   
                 protease 1 
                   
                   
               
               
                   
               
               
                 NM_002945 
                 Hs.461925 
                 RPA1 
                 Replication protein A1, 70 kDa 
                 5′-AACUGGUUGACGAAAGUGGUG-3′ 
                 492 
               
               
                   
               
               
                 NM_001184 
                 Hs.271791 
                 ATR 
                 Ataxia telangiectasia and Rad3 
                 5′-AACCCGCGUUGGCGUGGUUGA-3′ 
                 493 
               
               
                   
                   
                   
                 related 
                   
                   
               
               
                   
               
               
                 NM_001430.3 
                 Hs.468410 
                 EPAS1 
                 Endothelial PAS domain 
                 5′-ACCAAUCCAGCACCCAUCCdTdT-3′ 
                 494 
               
               
                   
                   
                   
                 protein 1 
                   
                   
               
               
                   
               
               
                 NM_001530.2 
                 Hs.509554 
                 HIF1A 
                 Hypoxia-inducible factor 1, 
                 5′-CUGAUGACCAGCAACUUGAdTdT-3′ 
                 495 
               
               
                   
                   
                   
                 alpha subunit (basic helix-loop- 
                   
                   
               
               
                   
                   
                   
                 helix transcription factor) 
                   
                   
               
               
                   
               
               
                 NM_021972 
                 Hs.68061 
                 SPHK1 
                 Sphingosine kinase 1 
                 5′-GAGCUGCAAGGCCUUGCCCdTdT-3 
                 496 
               
               
                   
               
               
                 NM_002502 
                 Hs.73090 
                 NFKB2 
                 Nuclear factor of kappa light 
                 5′-CTCCTCCATTGTGGAACCCAAGG 
                 497 
               
               
                   
                   
                   
                 polypeptide gene enhancer in 
                 AGC-3′ 
                   
               
               
                   
                   
                   
                 B-cells 2 (p49/p100) 
                   
                   
               
               
                   
               
               
                 NM_016829 
                 Hs.380271 
                 OGG1 
                 8-oxoguanine DNA 
                 5′-GUAUGGACACUGACUCAGAUU-3′ 
                 498 
               
               
                   
                   
                   
                 glycosylase 
                   
                   
               
               
                   
               
               
                 NM_016829 
                 Hs.380271 
                 OGG1 
                 8-oxoguanine DNA 
                 5′-GUACUUCCAGCUAGAUGUUUU-3′ 
                 499 
               
               
                   
                   
                   
                 glycosylase 
                   
                   
               
               
                   
               
               
                 NM_006142 
                 Hs.523718 
                 SFN 
                 Stratifin 
                 5′-GAGCGAAACCUGCUCUCAG-3′ 
                 500 
               
               
                   
               
               
                 NM_006142 
                 Hs.523718 
                 SFN 
                 Stratifin 
                 5′-GGGUGACUACUACCGCUAC-3′ 
                 501 
               
               
                   
               
               
                 NM_006142 
                 Hs.523718 
                 SFN 
                 Stratifin 
                 5′-AGACAGCACCCUCAUCAUG-3′ 
                 502 
               
               
                   
               
               
                 NM_00615 
                 Hs.477693 
                 NCK1 
                 NCK adaptor protein 1 
                 5′-GUCCUGGUGGC3AGUUCGATT-3′ 
                 503 
               
               
                   
               
               
                 NM_00615 
                 Hs.477693 
                 NCK1 
                 NCK adaptor protein 1 
                 5′-CGUCUCUAUGACCUCAACATT-3′ 
                 504 
               
               
                   
               
               
                 NM_002422 
                 Hs.375129 
                 MMP3 
                 Matrix metalloproteinase 3 
                 5′-AUGAAGAGUCUUCCAAUCCUU-3′ 
                 505 
               
               
                   
                   
                   
                 (stromelysin 1, progelatinase) 
                   
                   
               
               
                   
               
               
                 NM_000021.2 
                 Hs.3260 
                 PSEN1 
                 Presenilin 1 (Alzheimer 
                 5′-AAGGTCCACTTCGTATGCTGG-3′ 
                 506 
               
               
                   
                   
                   
                 disease 3) 
                   
                   
               
               
                   
               
               
                 NM_015331 
                 Hs.517249 
                 NCSTN 
                 Nicastrin 
                 5′-AAGGGCAAGTTTCCCGTGCAG-3′ 
                 507 
               
               
                   
               
               
                 NM_016022 
                 Hs.108408 
                 APH-1A 
                 Anterior pharynx defective 1 
                 5′-AAGAAGGCAGATGAGGGGTTA-3′ 
                 508 
               
               
                   
                   
                   
                 homolog A ( C. elegans ) 
                   
                   
               
               
                   
               
               
                 NM_172341 
                 Hs.534465 
                 PEN2 
                 Presenilin enhancer 2 homolog 
                 5′-AAUCAAAGGCUAUGUCUGGCG-3′ 
                 509 
               
               
                   
                   
                   
                 ( C. elegans ) 
                   
                   
               
               
                   
               
               
                 NM_020673 
                 Hs.529044 
                 RAB22A 
                 RAB22A, member RAS 
                 5′-AAGGACUACGCCGACUCUAUU-3′ 
                 510 
               
               
                   
                   
                   
                 oncogene family 
                   
                   
               
               
                   
               
               
                 NM_001002814 
                 Hs.191179 
                 RAB11FIP1 
                 RAB11 family interacting 
                 5′-CGCCTCTTTCCCAGTCCATGT-3′ 
                 511 
               
               
                   
                   
                   
                 protein 1 (class I) 
                   
                   
               
               
                   
               
               
                 NM_015470 
                 Hs.24557 
                 RAB11FIP5 
                 RAB11 family inetracting 
                 5′-GAGCTGAGTGCTCAGGCTAAA-3′ 
                 512 
               
               
                   
                   
                   
                 protein 5 (class I) 
                   
                   
               
               
                   
               
               
                 NM_030791 
                 Hs.24678 
                 SGPP1 
                 Sphingosine-1-phosphate 
                 5′-AGUGGCCCGUUUCCAGCGGdTT-3′ 
                 513 
               
               
                   
                   
                   
                 phosphatase 1 
                   
                   
               
               
                   
               
               
                 NM_005406 
                 Hs.306307 
                 ROCK1 
                 Rho-associated, coiled-coil 
                 5′-AAGGTGATTGGTAGAGGTGCA-3′ 
                 514 
               
               
                   
                   
                   
                 containing protein kinase 1 
                   
                   
               
               
                   
               
               
                 NM_198437 
                 Hs.250822 
                 STK6 
                 Serine/threonine kinase 6 
                 5′-AAGCACAAAAGCTTGTCTCCA-3′ 
                 515 
               
               
                   
               
               
                 NM_006272 
                 Hs.422181 
                 S100B 
                 S100 calcium binding protein, 
                 5′-GGAAUUCAUGGCCUUUGUU-3′ 
                 516 
               
               
                   
                   
                   
                 beta (neural) 
                   
                   
               
               
                   
               
               
                 NM_004219 
                 Hs.350966 
                 PTTG1 
                 Pituitary tumor-transforming 1 
                 5′-GAU CUC AAG UUU CAA CAC  
                 517 
               
               
                   
                   
                   
                   
                 Ctt-3′ 
                   
               
               
                   
               
               
                 NM_004219 
                 Hs.350966 
                 PTTG1 
                 Pituitary tumor-transforming 1 
                 5′-GUC UGU AAA GAC CAA GG  
                 518 
               
               
                   
                   
                   
                   
                 GAtt-3′ 
                   
               
               
                   
               
               
                 NM_001478.2 
                 Hs.159481 
                 GALGT 
                 UDP-N-acetyl-alpha-D- 
                 5′-GGAGCAAGUAGUGGGGCUGdTdT-3′ 
                 519 
               
               
                   
                   
                   
                 galactosamine:(N- 
                   
                   
               
               
                   
                   
                   
                 acetylneuraminyl)- 
                   
                   
               
               
                   
                   
                   
                 galactosylglueosylceramide N- 
                   
                   
               
               
                   
                   
                   
                 acetylgalactosaminyltransferase 
                   
                   
               
               
                   
               
               
                 NM_000657 
                 Hs.150749 
                 BCL2 
                 B-cell CLL/lymphoma 2 
                 5′-GUACAUCCAUUAUAAGCUGTT-3′ 
                 520 
               
               
                   
               
               
                 NM_032984 
                 Hs.368982 
                 CASP2 
                 Caspase 2, apoptosis-related 
                 5′-AACTTCCAGCTGGCATATAGGdTdT-3′ 
                 521 
               
               
                   
                   
                   
                 cysteine protease 
                   
                   
               
               
                   
               
               
                 NM_001228 
                 Hs.369736 
                 CASP8 
                 Caspase 8, apoptosis-related 
                 5′-AAGGGUCAUGCUCUAUCAGAUdTdT-3′ 
                 522 
               
               
                   
                   
                   
                 cysteine protease 
                   
                   
               
               
                   
               
               
                 NM_197967 
                 Hs.474150 
                 BID 
                 BH3 interacting domain death 
                 5′-AAGAAGACAUCAUCCGGAAUAdTdT-3′ 
                 523 
               
               
                   
                   
                   
                 agonist 
                   
                   
               
               
                   
               
               
                 NM_001167 
                 Hs.356076 
                 BIRC4 
                 Baculoviral IAP repeat- 
                 5′-AAGGAGAUACCGUGCGGUGCUdTdT-3′ 
                 524 
               
               
                   
                   
                   
                 containing 4 
                   
                   
               
               
                   
               
               
                 NM_002483 
                 Hs.466814 
                 CEACAM6 
                 Carcinoembryonic antigen- 
                 5′-CCGGACAGTTCCATGTATA-3′ 
                 525 
               
               
                   
                   
                   
                 related cell adhesion 
                   
                   
               
               
                   
                   
                   
                 molecule 6 
                   
                   
               
               
                   
               
               
                 NM_001008490 
                 Hs.285313 
                 KLF6 
                 Kruppel-like factor 6 
                 5′-GGAGAAAAGCCUUACAGAUTT-3′ 
                 526 
               
               
                   
               
               
                 NM_024309 
                 Hs.368551 
                 TNIP2 
                 TNFAIP3 interacting protein 2 
                 5′-GUAUUUGGCCGCCGACGCAd(TT)-3′ 
                 527 
               
               
                   
               
               
                 NM_001621 
                 Hs.171189 
                 AHR 
                 Aryl hydrocarbon receptor 
                 5′-AAGACTGGAGAAAGTGGCATG-3′ 
                 528 
               
               
                   
               
               
                 NM_001005845 
                 Hs.2442 
                 ADAM9 
                 A disintegrin and 
                 5′-AAUCACUGUGGAGACAUUUGCdTdT-3′ 
                 529 
               
               
                   
                   
                   
                 metalloproteinase domain 9 
                   
                   
               
               
                   
                   
                   
                 (meltrin gamma) 
                   
                   
               
               
                   
               
               
                 NM_001110 
                 Hs.172028 
                 ADAM10 
                 A disintegrin and 
                 5′-AAUGAAGAGGGACACUUCCCUdTdT-3′ 
                 530 
               
               
                   
                   
                   
                 metalloproteinase domain 10 
                   
                   
               
               
                   
               
               
                 NM_021641 
                 Hs.386283 
                 ADAM12 
                 A disintegrin and 
                 5′-AACCUCGCUGCAAAGAAUGUGdTdT-3′ 
                 531 
               
               
                   
                   
                   
                 metalloproteinase domain 12 
                   
                   
               
               
                   
               
               
                 NM_207196 
                 Hs.312098 
                 ADAM15 
                 A disintegrin and 
                 5′-AACUCCAUCUGUUCUCCUGACdTdT-3′ 
                 532 
               
               
                   
                   
                   
                 metalloproteinase domain 15 
                   
                   
               
               
                   
                   
                   
                 (metargidin) 
                   
                   
               
               
                   
               
               
                 NM_021832 
                 Hs.404914 
                 ADAM17 
                 A disintegrin and 
                 5′-AAAGUUUGCUUGGCACACCUUdTdT-3′ 
                 533 
               
               
                   
                   
                   
                 metalloproteinase domain 17 
                   
                   
               
               
                   
               
               
                 NM_000927.3 
                 Hs.489033 
                 ABCB1 
                 ATP-binding cassette, sub- 
                 5′-AAG GCC TAA TGC CGA ACA  
                 534 
               
               
                   
                   
                   
                 family B (MDR/TAP), member 1 
                 CA-3′ 
                   
               
               
                   
               
               
                 NM_000927.3 
                 Hs.489033 
                 ABCB1 
                 ATP-binding cassette, sub- 
                 5′-AAC TTT GGC TGC CAT CAT  
                 535 
               
               
                   
                   
                   
                 family B (MDR/TAP), member 1 
                 CCA-3′ 
                   
               
               
                   
               
               
                 NM_000572 
                 Hs.193717 
                 IL10 
                 Interleukin 10 
                 5′-UAAGCUCCAAGAGAAAGGCdTdT-3′ 
                 536 
               
               
                   
               
               
                 NM_021975 
                 Hs.502875 
                 RELA 
                 V-rel reticuloendotheliosis  
                 5′-GCCCUAUCCCUUUACGUCA-3′ 
                 537 
               
               
                   
                   
                   
                 viral oncogene homolog A 
                   
                   
               
               
                   
               
               
                 NM_001331 
                 Hs.166011 
                 CTNND1 
                 Catenin (cadherin-associated 
                 5′-GTGGACCATGCACTGCATGCCTAT 
                 538 
               
               
                   
                   
                   
                 protein), data 1 
                 AGTGAGTCGTATTAC-3′ 
                   
               
               
                   
               
               
                 NM_001211 
                 Hs.36708 
                 BUB1B 
                 BUB1 budding uninhibited by 
                 5′- AGATCCTGGCTAACTGTTC-3′ 
                 539 
               
               
                   
                   
                   
                 benzimidazoles 1 homolog beta 
                   
                   
               
               
                   
               
               
                 NM_002358 
                 Hs.533185 
                 MAD2L1 
                 MAD2 mitotic arrest deficient- 
                 5′-TACGGACTCACCTTGCTTG-3′ 
                 540 
               
               
                   
                   
                   
                 like 1 (yeast) 
                   
                   
               
               
                   
               
               
                 NM_001530.2 
                 Hs.509554 
                 HIF1A 
                 Hypoxia-inducible factor 1, 
                 5′-CUGGACACAGUGUGUUUGAdTdT-3′ 
                 541 
               
               
                   
                   
                   
                 alpha subunit 
                   
                   
               
               
                   
               
               
                 NM_001530.2 
                 Hs.509554 
                 HIF1A 
                 Hypoxia-inducible factor 1, 
                 5′-CUGAUGACCAGCAACUUGAdTdT-3′ 
                 542 
               
               
                   
                   
                   
                 alpha subunit 
                   
                   
               
               
                   
               
               
                 NM_001430 
                 Hs.468410 
                 EPAS1 
                 Endothelial PAS domain 
                 5′-GCUCUUCGCCAUGGACACAdTdT-3′ 
                 543 
               
               
                   
                   
                   
                 protein 1 
                   
                   
               
               
                   
               
               
                 NM_001430 
                 Hs.468410 
                 EPAS1 
                 Endothelial PAS domain 
                 5′-GCGACAGCUGGAGUAUGAAdTdT-3′ 
                 544 
               
               
                   
                   
                   
                 protein 1 
                   
                   
               
               
                   
               
               
                 NM_001379 
                 Hs.202672 
                 DNMT1 
                 DNA (cytosine-5-)- 
                 5′-CCAUGAGCACCGUUCUCCTT-3′ 
                 545 
               
               
                   
                   
                   
                 methyltransferase 1 
                   
                   
               
               
                   
               
               
                 NM_031310 
                 Hs.107125 
                 PLVAP 
                 Plasmalemma vesicle 
                 5′-CUUGACCAAGGAGCUCAACTT-3′ 
                 546 
               
               
                   
                   
                   
                 associated protein 
                   
                   
               
               
                   
               
               
                 NM_031310 
                 Hs.107125 
                 PLVAP 
                 Plasmalemma vesicle 
                 5′-GGAGCUCAACUUCACCACCTT-3′ 
                 547 
               
               
                   
                   
                   
                 associated protein 
                   
                   
               
               
                   
               
               
                 NM_016734 
                 Hs.126365 
                 PAX5 
                 Paired box gene 5 (B-cell 
                 5′-CGGCCACUCGCUUCCGGGCTT-3′ 
                 548 
               
               
                   
                   
                   
                 lineage specific activator) 
                   
                   
               
               
                   
               
               
                 NM_016734 
                 Hs.126365 
                 PAX5 
                 Paired box gene 5 (B-cell 
                 5′-GCUCCGUCGACUGCGCGCC-3′ 
                 549 
               
               
                   
                   
                   
                 lineage specific activator) 
                   
                   
               
               
                   
               
               
                 NM_006257 
                 Hs.498570 
                 PRKCQ 
                 Protein kinase C, theta 
                 5′-AAACCACCGTGGAGCTCTACT-3′ 
                 550 
               
               
                   
               
               
                 NM_006257 
                 Hs.498570 
                 PRKCQ 
                 Protein kinase C, theta 
                 5′-AAGAGCCCGACCTTCTGTGAA-3′ 
                 551 
               
               
                   
               
               
                 NM_032430 
                 Hs.182081 
                 BRSK1 
                 BR serine/threonine kinase 1 
                 5′-GUU CUU CCG CCA GAU UGU  
                 552 
               
               
                   
                   
                   
                   
                 GdTdT-3′ 
                   
               
               
                   
               
               
                 NM_015045 
                 Hs.203099 
                 KIAA0261 
                 KIAA0261 
                 5′-CGGACUACCCUUAGCACAAUU-3′ 
                 553 
               
               
                   
               
               
                 NM_015045 
                 Hs.203099 
                 KIAA0261 
                 KIAA0261 
                 5′-GAAUAGUCACCAUAUUCACUU-3′ 
                 554 
               
               
                   
               
               
                 NM_005430 
                 Hs.248164 
                 WNT1 
                 Wingless-type MMTV 
                 5′-GGTTCCATCGAATCCTGCA-3′ 
                 555 
               
               
                   
                   
                   
                 integration site family, 
                   
                   
               
               
                   
                   
                   
                 member 1 
                   
                   
               
               
                   
               
               
                 NM_004421.2 
                 Hs.74375 
                 DVL1 
                 Dishevelled, dsh homolog 1 
                 5′-AACAAGATCACCTTCTCCGAG-3′ 
                 556 
               
               
                   
                   
                   
                 (Drosophila) 
                   
                   
               
               
                   
               
               
                 NM_004422 
                 Hs.118640 
                 DVL2 
                 Dishevelled, dsh homolog 2 
                 5′-AACTTTGAGAACATGAGCAAC-3′ 
                 557 
               
               
                   
                   
                   
                 (Drosophila) 
                   
                   
               
               
                   
               
               
                 NM_139049 
                 Hs.522924 
                 MAPK8 
                 Mitogen-activated protein 
                 5′-CGTGGATTTATGGTCTGTG-3′ 
                 558 
               
               
                   
                   
                   
                 kinase 8 
                   
                   
               
               
                   
               
               
                 NM_003376 
                 Hs.73793 
                 VEGF 
                 Vascular endothelial growth 
                 5′-UGGAUGUCUAUCAGCGCAGdTdT-3′ 
                 559 
               
               
                   
                   
                   
                 factor 
                   
                   
               
               
                   
               
               
                 NM_003376 
                 Hs.73793 
                 VEGF 
                 Vascular endothelial growth 
                 5′-GCUACUGCCAUCCAAUCGAdTdT-3′ 
                 560 
               
               
                   
                   
                   
                 factor 
                   
                   
               
               
                   
               
               
                 NM_003376 
                 Hs.73793 
                 VEGF 
                 Vascular endothelial growth 
                 5′-GGAGUACCCUGAUGAGAUCdTdT-3′ 
                 561 
               
               
                   
                   
                   
                 factor 
                   
                   
               
               
                   
               
               
                 NM_003376 
                 Hs.73793 
                 VEGF 
                 Vascular endothelial growth 
                 5′-CUGAGGAGUCCAACAUCACdTdT-3′ 
                 562 
               
               
                   
                   
                   
                 factor 
                   
                   
               
               
                   
               
               
                 NM_003376 
                 Hs.73793 
                 VEGF 
                 Vascular endothelial growth 
                 5′-CCAAGGCCAGCACAUAGGAdTdT-3′ 
                 563 
               
               
                   
                   
                   
                 factor 
                   
                   
               
               
                   
               
               
                 NM_005123 
                 Hs.282735 
                 NR1H4 
                 Nuclear receptor subfamily 1, 
                 5′-GTCGTGACTTGCGACAAG-3′ 
                 564 
               
               
                   
                   
                   
                 group H, member 4 
                   
                   
               
               
                   
               
               
                 NM_004999 
                 Hs.149387 
                 MYO6 
                 Myosin VI 
                 5′-GCUGGCAGUUCAUAGGAAUdTdT-3′ 
                 565 
               
               
                   
               
               
                 NM_004999 
                 Hs.149387 
                 MYO6 
                 Myosin VI 
                 5′-CGUGCUCCAAAGUCUGU1AdTdT-3′ 
                 566 
               
               
                   
               
               
                 NM_014865 
                 Hs.5719 
                 CNAP1 
                 Chromosome condensation- 
                 5′-UCAGUAUGUUGUGCAAGAGTT-3′ 
                 567 
               
               
                   
                   
                   
                 related SMC-associated 
                   
                   
               
               
                   
                   
                   
                 protein 1 
                   
                   
               
               
                   
               
               
                 NM_014865 
                 Hs.5719 
                 CNAP1 
                 Chromosome condensation- 
                 5′-GAAGAUACUCUGGAAUUCCTT-3′ 
                 568 
               
               
                   
                   
                   
                 related SMC-associated 
                   
                   
               
               
                   
                   
                   
                 protein 1 
                   
                   
               
               
                   
               
               
                 NM_015261 
                 Hs.438550 
                 KIAA0056 
                 KIAA0056 protein 
                 5′-CUGGAUUUCACAGAGACUGTT-3′ 
                 569 
               
               
                   
               
               
                 NM_015261 
                 Hs.438550 
                 KIAA0056 
                 KIAA0056 protein 
                 5′-GCAGAGAUCAUAGAGACUGTT-3′ 
                 570 
               
               
                   
               
               
                 NM_015341 
                 Hs.308045 
                 BRRN1 
                 Barren homolog (Drosophila) 
                 5′-GACUUUCCUCAGAAUGACGTT-3′ 
                 571 
               
               
                   
               
               
                 NM_015341 
                 Hs.308045 
                 BRRN1 
                 Barren homolog (Drosophila) 
                 5′-CAUUACUCCACCUGUAUCATT-3′ 
                 572 
               
               
                   
               
               
                 NM_014551 
                 Hs.180903 
                 384D8-2 
                 hypothetical protein 384D8_6 
                 5′-GGAUUUCAGGAUGAACACGTT-3′ 
                 573 
               
               
                   
               
               
                 NM_014551 
                 Hs.180903 
                 384D8-2 
                 hypothetical protein 384D8_6 
                 5′-GCUGCAGGACUUCCACCAGTT-3′ 
                 574 
               
               
                   
               
               
                 NM_006031 
                 Hs.474069 
                 PCNT2 
                 Pericentrin 2 (kendrin) 
                 5′-AAUUGGAACAGCUGCAGCAGA-3′ 
                 575 
               
               
                   
               
               
                 NM_006031 
                 Hs.474069 
                 PCNT2 
                 Pericentrin 2 (kendrin) 
                 5′-AAGCUCUGAUUUAUCAAAAGA-3′ 
                 576 
               
               
                   
               
               
                 NM_012179.2 
                 Hs.5912 
                 FBXO7 
                 F-box protein 7 
                 5′-CCCACACCAUUCCAUUCUA-3′ 
                 577 
               
               
                   
               
               
                 NM_002467 
                 Hs.202453 
                 MYC 
                 V-myc myelocytomatosis viral 
                 5′-AAGAUGAGGAAGAAAUCGAUGUU-3′ 
                 578 
               
               
                   
                   
                   
                 oncogene homolog (avian) 
                   
                   
               
               
                   
               
               
                 NM_002467 
                 Hs.202453 
                 MYC 
                 V-myc myelocytomatosis viral 
                 5′-AAAAGGUCAGAGUCUGGAUCACC-3′ 
                 579 
               
               
                   
                   
                   
                 oncogene homolog (avian) 
                   
                   
               
               
                   
               
               
                 NM_002467 
                 Hs.202453 
                 MYC 
                 V-myc myelocytomatosis viral 
                 5′-CACGUCUCCACACAUCAGCACAA-3′ 
                 580 
               
               
                   
                   
                   
                 oncogene homolog (avian) 
                   
                   
               
               
                   
               
               
                 NM_002467 
                 Hs.202453 
                 MYC 
                 V-myc myelocytomatosis viral 
                 5′-AAAUGAGAUAAAGGUGGCUAAUU-3′ 
                 581 
               
               
                   
                   
                   
                 oncogene homolog (avian) 
                   
                   
               
               
                   
               
               
                 NM_002392 
                 Hs.369849 
                 MDM2 
                 Mdm2, transformed 3T3 cell 
                 5′-UGGUUGCAUUGUCCAUGGC-3′ 
                 582 
               
               
                   
                   
                   
                 double minute 2, p53 binding 
                   
                   
               
               
                   
                   
                   
                 protein 
                   
                   
               
               
                   
               
               
                 NM_003121 
                 Hs.437905 
                 SPIB 
                 Spi-B transcription factor 
                 5′-GATCGCTGTGTGTCTGTAA-3′ 
                 583 
               
               
                   
                   
                   
                 (Spi-1/PU.1 related) 
                   
                   
               
               
                   
               
               
                 NM_003120.1 
                 Hs.502511 
                 SPI1 
                 Spleen focus forming virus 
                 5′-GTCCGTATGTAAATCAGAT-3′ 
                 584 
               
               
                   
                   
                   
                 (SFFV) proviral integration 
                   
                   
               
               
                   
                   
                   
                 oncogene spi1 
                   
                   
               
               
                   
               
               
                 NM_199002 
                 Hs.278186 
                 ARHGEF1 
                 Rho guanine nucleotide 
                 5′-CATACCATCTCTACCGACG-3′ 
                 585 
               
               
                   
                   
                   
                 exchange factor (GEF) 1 
                   
                   
               
               
                   
               
               
                 NM_014784 
                 Hs.516954 
                 ARHGEF11 
                 Rho guanine nucleotide 
                 5′-ACTGAAGTCTCGGCCAGCT-3′ 
                 586 
               
               
                   
                   
                   
                 exchange factor (GEF) 11 
                   
                   
               
               
                   
               
               
                 NM_015313 
                 Hs.24598 
                 ARHGEF12 
                 Rho guanine nucleotide 
                 5′-GAAACTCGTCGCATCTTCC-3′ 
                 587 
               
               
                   
                   
                   
                 exchange factor (GEF) 12 
                   
                   
               
               
                   
               
               
                 NM_173842 
                 Hs.81134 
                 IL1RN 
                 Interleukin 1 receptor 
                 5′-AUCUGCAGAGGCCUCCGCAtt-3′ 
                 588 
               
               
                   
                   
                   
                 antagonist 
                   
                   
               
               
                   
               
               
                 NM_032726 
                 Hs.549218 
                 PLCD4 
                 Phospholipase C, delta 4 
                 5′-GAGCAGAACCTTCAGAATAdTdT-3′ 
                 589 
               
               
                   
               
               
                 NM_032726 
                 Hs.549218 
                 PLCD4 
                 Phospholipase C, delta 4 
                 5′-GAGCAGGGCTTCACCATTGdTdT-3′ 
                 590 
               
               
                   
               
               
                 NM_032726 
                 Hs.549218 
                 PLCD4 
                 Phospholipase C, delta 4 
                 5′-GGAAGGAGAAGAATTCGTAdTdT-3′ 
                 591 
               
               
                   
               
               
                 NM_032726 
                 Hs.549218 
                 PLCD4 
                 Phospholipase C, delta 4 
                 5′-GATATCATCTTTCTCTGAAdTdT-3′ 
                 592 
               
               
                   
               
               
                 NM_004104 
                 Hs.83190 
                 FASN 
                 Fatty acid synthase 
                 5′-CAACTACGGCTTTGCCAAT-3′ 
                 593 
               
               
                   
               
               
                 NM_004104 
                 Hs.83190 
                 FASN 
                 Fatty acid synthase 
                 5′-GCAACTCACGCTCCGGAAA-3′ 
                 594 
               
               
                   
               
               
                 NM_004104 
                 Hs.83190 
                 FASN 
                 Fatty acid synthase 
                 5′-GCCCTGAGCTGGACTACTT-3′ 
                 595 
               
               
                   
               
               
                 NM_004104 
                 Hs.83190 
                 FASN 
                 Fatty acid synthase 
                 5′-GGTATGCGACGGGAAAGTA-3′ 
                 596 
               
               
                   
               
               
                 NM_002165.2 
                 Hs.504609 
                 ID1 
                 Inhibitor of DNA binding 1, 
                 5′-AACTCGGAATCCGAAGTTGGA-3′ 
                 597 
               
               
                   
                   
                   
                 dominant negative helix-loop- 
                   
                   
               
               
                   
                   
                   
                 helix protein 
                   
                   
               
               
                   
               
               
                 NM_003200.1 
                 Hs.371282 
                 TCF3 
                 Transcription factor 3 
                 5′-AAAGACCTGAGGGACCGGGAG-3′ 
                 598 
               
               
                   
               
               
                 NM_015895 
                 Hs.234896 
                 GMNN 
                 Geminin, DNA replication 
                 5′-GAGAAAATGAGCTGTCCGC-3′ 
                 599 
               
               
                   
                   
                   
                 inhibitor 
                   
                   
               
               
                   
               
               
                 NM_015895 
                 Hs.234896 
                 GMNN 
                 Geminin, DNA replication 
                 5′-CTGGCAGAAGTAGCAGAAC-3′ 
                 600 
               
               
                   
                   
                   
                 inhibitor 
                   
                   
               
               
                   
               
               
                 NM_006704 
                 Hs.281902 
                 SUGT1 
                 SGT1, suppressor of G2 allele 
                 5′-AAGGCUUUGGAACAGAAACCA-3′ 
                 601 
               
               
                   
                   
                   
                 of SKP1 ( S. cerevisiae ) 
                   
                   
               
               
                   
               
               
                 NM_002358 
                 Hs.533185 
                 MAD2L1 
                 MAD2 mitotic arrest deficient- 
                 5′-AAGAGUCGGGACCACAGUUUA-3′ 
                 602 
               
               
                   
                   
                   
                 like 1 
                   
                   
               
               
                   
               
               
                 NM_006472 
                 Hs.533977 
                 TXNIP 
                 Thioredoxin interacting 
                 5′-ACAGACUUCGGAGUACCUG1TT-3′ 
                 603 
               
               
                   
                   
                   
                 protein 
                   
                   
               
               
                   
               
               
                 NM_001379 
                 Hs.202672 
                 DNMT1 
                 DNA (cytosine-5-)- 
                 5′-CGGUGCUCAUGCUUACAACTT-3′ 
                 604 
               
               
                   
                   
                   
                 methyltransferase 1 
                   
                   
               
               
                   
               
               
                 NM_001379 
                 Hs.202672 
                 DNMT1 
                 DNA (cytosine-5-)- 
                 5′-CGAGUUGCUAGACCGCUUCTT-3′ 
                 605 
               
               
                   
                   
                   
                 methyltransferase 1 
                   
                   
               
               
                   
               
               
                 NM_006838 
                 Hs.444986 
                 METAP2 
                 Methionyl aminopeptidase 2 
                 5′-AAUGCCGGUGACACAACAUGA-3′ 
                 606 
               
               
                   
               
               
                 NM_007862.2 
                 Mm.382 
                 Dlgh1 
                 Discs, large homolog 1 
                 5′-TACGGGAGCAGATGATGAAA-3′ 
                 607 
               
               
                   
                   
                   
                 (Drosophila) 
                   
                   
               
               
                   
               
               
                 NM_007862.2 
                 Mm.382 
                 Dlgh1 
                 Discs, large homolog 1 
                 5′-AACCCAAATCCATGGAAAATA-3′ 
                 608 
               
               
                   
                   
                   
                 (Drosophila) 
                   
                   
               
               
                   
               
               
                 NM_008173.1 
                 Mm.129481 
                 Nr3c1 
                 Nuclear receptor subfamily 3, 
                 5′-GAGCAGTGGAAGGACAGCATTCAA 
                 609 
               
               
                   
                   
                   
                 group C, member 1 
                 GAGATGCTGTCCTTCCACTGCTCTTTT 
                   
               
               
                   
                   
                   
                   
                 TT-3′ 
                   
               
               
                   
               
               
                 NM_008173.1 
                 Mm.129481 
                 Nr3c1 
                 Nuclear receptor subfamily 3, 
                 5′-GATCCCGAGCAGTGGAAGGACAGC 
                 610 
               
               
                   
                   
                   
                 group C, member 1 
                 ATTCAAGAGATGCTGTCCTTCCACTGC 
                   
               
               
                   
                   
                   
                   
                 TCTTTTTTGGAAA-3′ 
                   
               
               
                   
               
               
                 NM_007871.1 
                 Mm.39292 
                 Dnm2 
                 Dynamin 2 
                 5′-GGACCAGGCAGAGAATGAG-3′ 
                 611 
               
               
                   
               
               
                 NM_011155.1 
                 Mm.3294 
                 Ppp5c 
                 Protein phosphatase 5, 
                 5′-AAG ACA CAG GCC AAC GAC 
                 612 
               
               
                   
                   
                   
                 catalytic subunit 
                 UAC-3′ 
                   
               
               
                   
               
               
                 NM_011155.1 
                 Mm.3294 
                 Ppp5c 
                 Protein phosphatase 5, 
                 5′-AAG AUU GUG AAG CAG AAG 
                 613 
               
               
                   
                   
                   
                 catalytic subunit 
                 GCC-3′ 
                   
               
               
                   
               
               
                 NM_009263.1 
                 Mm.288474 
                 Spp1 
                 Secreted phosphoprotein 1 
                 5′-AATCTCCTTGCGCCACAGAAT-3′ 
                 614 
               
               
                   
               
               
                 NM_009263.1 
                 Mm.288474 
                 Sppl 
                 Secreted phosphoprotein 1 
                 5′-AAGTCAGCTGGATGAACCAAG-3′ 
                 615 
               
               
                   
               
               
                 NM_145978.1 
                 Mm.283968 
                 Pdlim2 
                 PDZ and LIM domain 2 
                 5′-AAGAUCCGACAGAGCGC05CA-3′ 
                 616 
               
               
                   
               
               
                 NM_146386.1 
                 Mm.32257 
                 Myocd 
                 Myocardin 
                 5′-AATGCAACTGCAGAAGCAGAA-3′ 
                 617 
               
               
                   
               
               
                 NM_145541.3 
                 Mm.333868 
                 Rap1a 
                 RAS-related protein-1a 
                 5′-AAGCAAGTCGAGGTAGATTGC-3′ 
                 618 
               
               
                   
               
               
                 NM_010026.1 
                 Mm.277236 
                 Ddef1 
                 RIKEN cDNA 1700010G06 
                 5′-CAGCUAACUGCACUCCGAG-3′ 
                 619 
               
               
                   
                   
                   
                 gene 
                   
                   
               
               
                   
               
               
                 NM_010026.1 
                 Mm.277236 
                 Ddef1 
                 RIKEN cDNA 1700010G06 
                 5′-UGAUAUUAUGGAAGCAAAU-3′ 
                 620 
               
               
                   
                   
                   
                 gene 
                   
                   
               
               
                   
               
               
                 NM_007614.2 
                 Mm.291928 
                 Catnb 
                 Catenin beta 
                 5′-AAGGCTTTTCCCAGTCCTTCA-3′ 
                 621 
               
               
                   
               
               
                 NM_007614.2 
                 Mm.291928 
                 Catnb 
                 Catenin beta 
                 5′-AAGATGATGGTGTGCCAAGTG-3′ 
                 622 
               
               
                   
               
               
                 NM_011952.1 
                 Mm.8385 
                 Mapk3 
                 Mitogen activated protein 
                 5′-AATGTTATAGGCATCCGAGAC-3′ 
                 623 
               
               
                   
                   
                   
                 kinase 3 
                   
                   
               
               
                   
               
               
                 NM_011952.1 
                 Mm.8385 
                 Mapk3 
                 Mitogen activated protein 
                 5′-AACCCAAACAAGCGCATCACA-3′ 
                 624 
               
               
                   
                   
                   
                 kinase 3 
                   
                   
               
               
                   
               
               
                 NM_011949.2 
                 Mm.196581 
                 Mapk1 
                 Mitogen activated protein 
                 5′-AAAGTTCGAGTTGCTATCAAG-3′ 
                 625 
               
               
                   
                   
                   
                 kinase 1 
                   
                   
               
               
                   
               
               
                 NM_010591.1 
                 Mm.275071 
                 Jun 
                 Jun oncogene 
                 5′-GCGCATGAGGAACCGCATT-3′ 
                 626 
               
               
                   
               
               
                 NM_008416.1 
                 Mm.1167 
                 Junb 
                 Jun-B oncogene 
                 5′-GACCAGGAGCGCATCAAAG-3′ 
                 627 
               
               
                   
               
               
                 NM_010592.3 
                 Mm.1175 
                 Jund1 
                 Jun proto-oncogene related 
                 5′-AAGCCAGAACACCGAGCTG-3′ 
                 628 
               
               
                   
                   
                   
                 gene d1 
                   
                   
               
               
                   
               
               
                 NM_010234.2 
                 Mm.246513 
                 Fos 
                 FBJ osteosarcoma related 
                 5′-GCGGAGACAGATCAACTTG-3′ 
                 629 
               
               
                   
                   
                   
                 oncogene 
                   
                   
               
               
                   
               
               
                 NM_010235.1 
                 Mm.6215 
                 Fosl1 
                 Fos-like antigen 1 
                 5′-ATTGGAGGATGAGAAATCG-3′ 
                 630 
               
               
                   
               
               
                 NM_008037.3 
                 Mm.24684 
                 Fosl2 
                 Fos-like antigen 2 
                 5′-TCAACGCCATCACCACCAG-3′ 
                 631 
               
               
                   
               
               
                 NM_009505.2 
                 Mm.282184 
                 Vegfa 
                 Vascular endothelial growth 
                 5′-AACGAUGAAGCCCUGGAGUGC-3′ 
                 632 
               
               
                   
                   
                   
                 factor A 
                   
                   
               
               
                   
               
               
                 NM_015774.2 
                 Mm.264435 
                 Ero11 
                 ERO1-like ( S. cerevisiae ) 
                 5′-ACTTCATCAGAATGGCAGGGTTT-3′ 
                 633 
               
               
                   
               
               
                 NM_021450.1 
                 Mm.244705 
                 Trpm7 
                 Transient receptor potential 
                 5′-AACCGGAGGTCAGGTCGAAAT-3′ 
                 634 
               
               
                   
                   
                   
                 cation channel, subfamily M, 
                   
                   
               
               
                   
                   
                   
                 member 7 
                   
                   
               
               
                   
               
               
                 NM_021450.1 
                 Mm.244705 
                 Trpm7 
                 Transient receptor potential 
                 5′-AAGCAGAGTGACCTGGTAGAT-3 
                 635 
               
               
                   
                   
                   
                 cation channel, subfamily M, 
                   
                   
               
               
                   
                   
                   
                 member 7 
                   
                   
               
               
                   
               
               
                 NM_177407.2 
                 Mm.131530 
                 Camk2a 
                 Calcium/calmodulin-dependent 
                 5′-CACCACCAUUGAGGACGAAdTdT-3′ 
                 636 
               
               
                   
                   
                   
                 protein kinase II alpha 
                   
                   
               
               
                   
               
               
                 NM_010118.1 
                 Mm.290421 
                 Egr2 
                 Early growth response 2 
                 5′-GUGACCACCUUACUACUCAdTdT-3′ 
                 637 
               
               
                   
               
               
                 NM_010118.1 
                 Mm.290421 
                 Egr2 
                 Early growth response 2 
                 5′-GUUUGCCAGGAGUGACGAAdTdT-3′ 
                 638 
               
               
                   
               
               
                 NM_015806.2 
                 Mm.18856 
                 Mapk6 
                 Mitogen-activated protein 
                 5′-GGCUUUUCAUGUAUCAGCUTT-3′ 
                 639 
               
               
                   
                   
                   
                 kinase 6 
                   
                   
               
               
                   
               
               
                 NM_015806.2 
                 Mm.18856 
                 Mapk6 
                 Mitogen-activated protein 
                 5′-GGCAAUGGCUUGGUUUUUUTT-3′ 
                 640 
               
               
                   
                   
                   
                 kinase 6 
                   
                   
               
               
                   
               
               
                 NM_015806.2 
                 Mm.18856 
                 Mapk6 
                 Mitogen-activated protein 
                 5′-GGAGUACAUGGAGACAGACTT-3′ 
                 641 
               
               
                   
                   
                   
                 kinase 6 
                   
                   
               
               
                   
               
               
                 NM_009744.2 
                 Mm.347398 
                 Bcl6 
                 B-cell leukemia/lymphoma 6 
                 5′-GTCGAGACATCTTGACTGA-3′ 
                 642 
               
               
                   
               
               
                 NM_009744.2 
                 Mm.347398 
                 Bcl6 
                 B-cell leukemia/lymphoma 6 
                 5′-GACACGGATCTGAGAATCT-3′ 
                 643 
               
               
                   
               
               
                 NM_145533.1 
                 Mm.136586 
                 Smox 
                 Spermine oxidase 
                 5′-GGACGUGGUUGAGGAAUUC-3′ 
                 644 
               
               
                   
               
               
                 NM_008778.1 
                 Mm.40035 
                 Pak3 
                 P21 (CDKN1A)-activated 
                 5′-TAGCAGCACATCAGTCGAATA-3′ 
                 645 
               
               
                   
                   
                   
                 kinase 3 
                   
                   
               
               
                   
               
               
                 NM_008778.1 
                 Mm.40035 
                 Pak3 
                 P21 (CDKN1A)-activated 
                 5′-CCCAATATTGTCAATTATTTA-3′ 
                 646 
               
               
                   
                   
                   
                 kinase 3 
                   
                   
               
               
                   
               
               
                 NM_080428.2 
                 Mm.196475 
                 Fbxw7 
                 F-box and WD-40 domain 
                 5′-CACAAAGCTGGTGTGTGCA-3′ 
                 647 
               
               
                   
                   
                   
                 protein 7, archipelago homolog 
                   
                   
               
               
                   
               
               
                 NM_021450.1 
                 Mm.244705 
                 Trpm7 
                 Transient receptor potential 
                 5′-AACCGGAGGTCAGGTCGAAAT-3′ 
                 648 
               
               
                   
                   
                   
                 cation channel, subfamily M,  
                   
                   
               
               
                   
                   
                   
                 member 7 
                   
                   
               
               
                   
               
               
                 NM_021450.1 
                 Mm.244705 
                 Trpm7 
                 Transient receptor potential 
                 5′-AAGCAGAGTGACCTGGTAGAT-3′ 
                 649 
               
               
                   
                   
                   
                 cation channel, subfamily M, 
                   
                   
               
               
                   
                   
                   
                 member 7 
                   
                   
               
               
                   
               
               
                 NM_009505.2 
                 Mm.282184 
                 Vegfa 
                 Vascular endothelial growth 
                 5′-AAGCCGTCCTGTGTGCCGCTG-3′ 
                 650 
               
               
                   
                   
                   
                 factor A 
                   
                   
               
               
                   
               
               
                 NM_009505.2 
                 Mm.282184 
                 Vegfa 
                 Vascular endothelial growth 
                 5′-AACGATGAAGCCCTGGAGTGC-3′ 
                 651 
               
               
                   
                   
                   
                 factor A 
                   
                   
               
               
                   
               
               
                 NM_010228.2 
                 Mm.3464 
                 Flt1 
                 FMS-like tyrosine kinase 1 
                 5′-AAGTTAAAAGTGCCTGAACTG-3′ 
                 652 
               
               
                   
               
               
                 NM_010228.2 
                 Mm.3464 
                 Flt1 
                 FMS-like tyrosine kinase 1 
                 5′-AAGCAGGCCAGACTCTCTTTC-3′ 
                 653 
               
               
                   
               
               
                 NM_010612.2 
                 Mm.285 
                 Kdr 
                 Kinase insert domain protein 
                 5′-AAGCTCAGCACACAGAAAGAC-3′ 
                 654 
               
               
                   
                   
                   
                 receptor 
                   
                   
               
               
                   
               
               
                 NM_010612.2 
                 Mm.285 
                 Kdr 
                 Kinase insert domain protein 
                 5′-AATGCGGCGGTGGTGACAGTA-3′ 
                 655 
               
               
                   
                   
                   
                 receptor 
                   
                   
               
               
                   
               
               
                 AK019429.1 
                 Mm.3049 
                 Cks1b 
                 CDC28 protein kinase 1b 
                 5′-GGGACATAGCCAAGCTGGTCgagt 
                 656 
               
               
                   
                   
                   
                   
                 actgGACCAGCTTGGCTATGTCC-3′ 
                   
               
               
                   
               
               
                 NM_008000.1 
                 Mm.280819 
                 Fert2 
                 Fer (fms/fps related) protein 
                 5′-AAC TAC GGT TGC TGG AGA 
                 657 
               
               
                   
                   
                   
                 kinase, testis specific 2 
                 CAG-3′ 
                   
               
               
                   
               
               
                 NM_009750.1 
                 Mm.90787 
                 Ngfrap1 
                 Nerve growth factor receptor 
                 5′-CAACAACCACAACCATAAC-3′ 
                 658 
               
               
                   
                   
                   
                 (TNFRSF16) associated 
                   
                   
               
               
                   
                   
                   
                 protein 1 
                   
                   
               
               
                   
               
               
                 NM_009750.1 
                 Mm.90787 
                 Ngfrap1 
                 Nerve growth factor receptor 
                 5′-CATAACCACAACCACCACTdTdT-3′ 
                 659 
               
               
                   
                   
                   
                 (TNFRSF16) associated 
                   
                   
               
               
                   
                   
                   
                 protein 1 
                   
                   
               
               
                   
               
               
                 NM_010431.1 
                 Mm.3879 
                 Hif1a 
                 Hypoxia inducible factor 1, 
                 5′-TGTGAGCTCACATCTTGAT-3′ 
                 660 
               
               
                   
                   
                   
                 alpha subunit 
                   
                   
               
               
                   
               
               
                 NM_010838 
                 Mm.1287 
                 Mapt 
                 Microtubule-associated protein 
                 5′-CCAGGAGTTTGACACAATG-3′ 
                 661 
               
               
                   
                   
                   
                 tau 
                   
                   
               
               
                   
               
               
                 NM_009045 
                 Mm.2499661 
                 Rela 
                 V-rel reticuloendotheliosis 
                 5′-GATCAATGGCTACACAGGA-3′ 
                 662 
               
               
                   
                   
                   
                 viral oncogene homolog A 
                   
                   
               
               
                   
               
               
                 NM_009689 
                 Mm.8552 
                 Birc5 
                 Baculoviral IAP repeat- 
                 5′-GAGCCAAGAACAAAATTGC-3′ 
                 663 
               
               
                   
                   
                   
                 containing 5 
                   
                   
               
               
                   
               
               
                 NM_009689 
                 Mm.8552 
                 Birc5 
                 Baculoviral IAP repeat- 
                 5′-GAAAGTGCGCCGTGCCATC-3′ 
                 664 
               
               
                   
                   
                   
                 containing 5 
                   
                   
               
               
                   
               
               
                 NM_007798.1 
                 Mm.236553 
                 CTSB 
                 Cathepsin B 
                 5′-CCACUGUGGCAUUGAAUCATT-3′ 
                 665 
               
               
                   
               
               
                 NM_011960 
                 Mm.15962 
                 Parg 
                 Poly (ADP-ribose) 
                 5′-AACGCCACCTCGTTTGTTTTC-3′ 
                 666 
               
               
                   
                   
                   
                 glycohydrolase 
                   
                   
               
               
                   
               
               
                 NM_010928.1 
                 Mm.254017 
                 Notch2 
                 Notch gene homolog 2 
                 5′-GAUGUGGACAGUGUCUGUTT-3′ 
                 667 
               
               
                   
               
               
                 NM_019984 
                 Mm.41964 
                 Tgm1 
                 Transglutaminase 1, K 
                 5′-AUGCAGCUGGAGAUGGCACdTdT-3′ 
                 668 
               
               
                   
                   
                   
                 polypeptide 
                   
                   
               
               
                   
               
               
                 NM_009593 
                 Mm.15691 
                 Abcg1 
                 ATP-binding cassette, sub- 
                 5′-CGTGGATGAGGTTGAGACA-3′ 
                 669 
               
               
                   
                   
                   
                 family G (WHITE), member 1 
                   
                   
               
               
                   
               
               
                 NM_009593 
                 Mm.15691 
                 Abcg1 
                 ATP-binding cassette, sub- 
                 5′-GGTGGACAACAACTTCACA-3′ 
                 670 
               
               
                   
                   
                   
                 family G (WHITE), member 1 
                   
                   
               
               
                   
               
               
                 NM_138955 
                 Mm.101876 
                 Abcg4 
                 ATP-binding cassette, sub- 
                 5′-GAAGGTGGAGAACCATATC-3′ 
                 671 
               
               
                   
                   
                   
                 family G (WHITE), member 4 
                   
                   
               
               
                   
               
               
                 NM_138955 
                 Mm.101876 
                 Abcg4 
                 ATP-binding cassette, sub- 
                 5′-GCACTTGAACTACTGGTAT-3′ 
                 672 
               
               
                   
                   
                   
                 family G (WHITE), member 4 
                   
                   
               
               
                   
               
               
                 NM_011658 
                 Mm.3280 
                 Twist1 
                 Twist gene homolog 1 
                 5′-AAGCTGAGCAAGATTCAGACC-3′ 
                 673 
               
               
                   
               
               
                 NM_011658 
                 Mm.3280 
                 Twist1 
                 Twist gene homolog 1 
                 5′-AGGTACATCGACTTCCTGTAC-3′ 
                 674 
               
               
                   
               
               
                 NM_011658 
                 Mm.3280 
                 Twist1 
                 Twist gene homolog 1 
                 5′-AGCGGGTCATGGCTAACGTGC-3′ 
                 675 
               
               
                   
               
               
                 NM_009537 
                 Mm.3868 
                 Yy1 
                 YY1 transcription factor 
                 5′-GGGAGCAGAAGCAGGUGCAGAU-3′ 
                 676 
               
               
                   
               
               
                 NM_172689 
                 Mm.86382 
                 Ddx58 
                 DEAD (Asp-Glu-Ala-Asp) box 
                 5′-GCCCATTGAAACCAAGAAATT-3′ 
                 677 
               
               
                   
                   
                   
                 polypeptide 58 
                   
                   
               
               
                   
               
               
                 NM_008500.1 
                 Mm.12881 
                 Lhx6 
                 LIM homeobox protein 6 
                 5′-AGACGCAGAGGCCTTGGTTCAAGAGA 
                 678 
               
               
                   
                   
                   
                   
                 CCAAGGCCTCTGCGTCTGACTTTTTC-3′ 
                   
               
               
                   
               
               
                 NM_009308 
                 Mm.233846 
                 Syt4 
                 Synaptotagmin 4 
                 5′-r(GAAGCACAGAGUGAAGACCA) 
                 679 
               
               
                   
                   
                   
                   
                 d(TT)-3′ 
                   
               
               
                   
               
               
                 NM_009308 
                 Mm.233846 
                 Syt4 
                 Synaptotagmin 4 
                 5′-r(CGAGCAGGAGAACAGCGAG) 
                 680 
               
               
                   
                   
                   
                   
                 d(TT)-3′ 
                   
               
               
                   
               
               
                 NM_007615.1 
                 Mm.35738 
                 Catns 
                 Expressed sequence AI225934 
                 5′-GATGGTTATCCAGGTGGCA-3′ 
                 681 
               
               
                   
               
               
                 NM_007614.2 
                 Mm.291928 
                 Catnb 
                 Catenin beta 
                 5′-CUGUUGUGGUUAAACUCCUTT-3′ 
                 682 
               
               
                   
               
               
                 NM_011232 
                 Mm.38376 
                 Rad1 
                 RAD1 homolog ( S. pombe ) 
                 5′-GCCTTGACAACGTTAGGAATC-3′ 
                 683 
               
               
                   
               
               
                 NM_011232 
                 Mm.38376 
                 Rad1 
                 RAD1 homolog ( S. pombe ) 
                 5′-GCAGGAAGTTCCCACCTTGAC-3′ 
                 684 
               
               
                   
               
               
                 NM_011232 
                 Mm.38376 
                 Rad1 
                 RAD1 homolog ( S. pombe ) 
                 5′-GCCTGATGAAGAAGTTCC-3′ 
                 685 
               
               
                   
               
               
                 NM_010423 
                 Mm.29581 
                 Hcy1 
                 Hairy/enhancer-of-split  
                 5′-GCTAGAAAAAGCTGAGATC-3′ 
                 686 
               
               
                   
                   
                   
                 related with YRPW motif 1 
                   
                   
               
               
                   
               
               
                 NM_010133 
                 Mm.2657 
                 En1 
                 Engrailed 1 
                 5′-CAUCCUAAGGCCCGAUUUCTT-3′ 
                 687 
               
               
                   
               
               
                 NM_010133 
                 Mm.2657 
                 En1 
                 Engrailed 1 
                 5′-GUUCCCGGAACACAACCCUTT-3′ 
                 688 
               
               
                   
               
               
                 NM_019390 
                 Mm.243014 
                 Lmna 
                 Lamin A 
                 5′-GCAGCUUCAGGAUGAGAUGTT-3′ 
                 689 
               
               
                   
               
               
                 NM_183355.1 
                 Mm.43358 
                 Pbx1 
                 Pre B-cell leukemia 
                 5′-CAGUUUUGAGUAUUCGGGGTT-3′ 
                 690 
               
               
                   
                   
                   
                 transcription factor 1 
                   
                   
               
               
                   
               
               
                 NM_007430 
                 Mm.5180 
                 Nr0b1 
                 Nuclear receptor subfamily 0, 
                 5′-GAUCACCUGCACUUCGAGdTdT-3′ 
                 691 
               
               
                   
                   
                   
                 group B, member 1 
                   
                   
               
               
                   
               
               
                 NM_007430 
                 Mm.5180 
                 Nr0b1 
                 Nuclear receptor subfamily 0, 
                 5′-CUGAACAGUGCCCUUUUCCdTdT-3′ 
                 692 
               
               
                   
                   
                   
                 group B, member 1 
                   
                   
               
               
                   
               
               
                 NM_172203 
                 Mm.233865 
                 Nox1 
                 NADPH oxidase 1 
                 5′-TTATGAGAAGTCTGACAAG-3′ 
                 693 
               
               
                   
               
               
                 NM_172203 
                 Mm.233865 
                 Nox1 
                 NADPH oxidase 1 
                 5′-GATTCTTGGCTAAATCCCA-3′ 
                 694 
               
               
                   
               
               
                 NM_172203 
                 Mm.233865 
                 Nox1 
                 NADPH oxidase 1 
                 5′-GGACATTTGAACAACAGCA-3′ 
                 695 
               
               
                   
               
               
                 NM_080850 
                 Mm.253287 
                 Pask 
                 PAS domain containing 
                 5′-AATTTATGGAGTCAACCACAGCTT-3′ 
                 696 
               
               
                   
                   
                   
                 serine/threonine kinase 
                   
                   
               
               
                   
               
               
                 NM_009330 
                 Mm.7226 
                 Tcf2 
                 Transcription factor 2 
                 5′-GCCGGUUUUCCAUACUCUCtt-3′ 
                 697 
               
               
                   
               
               
                 NM_009330 
                 Mm.7226 
                 Tcf2 
                 Transcription factor 2 
                 5′-CAAGAAGAUGCGCCGCAACtt-3′ 
                 698 
               
               
                   
               
               
                 NM_009330 
                 Mm.7226 
                 Tcf2 
                 Transcription factor 2 
                 5′-UGGUGGUCACAGAUACCAGtt-3′ 
                 699 
               
               
                   
               
               
                 NM_024148.1 
                 Rn.5949 
                 Apex1 
                 Apurinic/apyrimidinic 
                 5′-GUCUGGUAAGACUGGAGUACC-3′ 
                 700 
               
               
                   
                   
                   
                 endonuclease 1 
                   
                   
               
               
                   
               
               
                 NM_017059.1 
                 Rn.10668 
                 Bax 
                 Bcl2-associated X protein 
                 5′-UUGGAGAUGAACUGGACAAUU-3′ 
                 701 
               
               
                   
               
               
                 NM_017059.1 
                 Rn.10668 
                 Bax 
                 Bcl2-associated X protein 
                 5′-CUG GAC AAU AAU AUG GAG  
                 702 
               
               
                   
                   
                   
                   
                 CUU-3′ 
                   
               
               
                   
               
               
                 NM_023979.1 
                 Rn.64522 
                 Apaf1 
                 Apoptotic protease activating 
                 5′-AGA ACU UUG UGC UUU AAU  
                 703 
               
               
                   
                   
                   
                 factor 1 
                 GUU-3′ 
                   
               
               
                   
               
               
                 NM_023979.1 
                 Rn.64522 
                 Apaf1 
                 Apoptotic protease activating 
                 5′-UAU AGG CAU AUA CUG GAU  
                 704 
               
               
                   
                   
                   
                 factor 1 
                 GUU-3′ 
                   
               
               
                   
               
               
                 NM_021835.2 
                 Rn.93714 
                 Jun 
                 V-jun sarcoma virus 17 
                 5′-AGU GAA AAC CUU GAA AGC  
                 705 
               
               
                   
                   
                   
                 oncogene homolog (avian) 
                 GUU-3′ 
                   
               
               
                   
               
               
                 NM_021835.2 
                 Rn.93714 
                 Jun 
                 V-jun sarcoma virus 17 
                 5′-AGU CAU GAA CCA CGU UAA  
                 706 
               
               
                   
                   
                   
                 oncogene homolog (avian) 
                 CUU-3′ 
                   
               
               
                   
               
               
                 NM_012655.1 
                 Rn.44609 
                 Sp1 
                 Sp1 transcription factor 
                 AATGAGAACAGCAACAACTCC 
                 707 
               
               
                   
               
               
                 XM_230974.2 
                 Rn.102138 
                 Sp3 
                 Sp3 transcription factor 
                 AAGTTCTCAGACAATGACTGC 
                 708 
               
               
                   
               
               
                 NM_133551.1 
                 Rn.10162 
                 Pla2g4a 
                 Phospholipase A2, group IVA 
                 5′-TCGAGACAGTAGTGGTTCTACGTG 
                 709 
               
               
                   
                   
                   
                 (cytosolic, calcium-dependent) 
                 CCgagtactgGGCACGTAGAACCACTA 
                   
               
               
                   
                   
                   
                   
                 CTGTTTTTT-3′ 
                   
               
               
                   
               
               
                 NM_001003959.1 
                 Rn.117353 
                 Dnmt3b 
                 DNA methyltransferase 3B 
                 5′-AGAUGACAGGUGCCCAGAGUU-3′ 
                 710 
               
               
                   
               
               
                 NM_019335.1 
                 Rn.10022 
                 Prkr 
                 Protein kinase, interferon- 
                 5′-GGUAGAUCAAAGCAGGAGGTT-3′ 
                 711 
               
               
                   
                   
                   
                 inducible double stranded RNA  
                   
                   
               
               
                   
                   
                   
                 dependent 
                   
                   
               
               
                   
               
               
                 NM_053622.1 
                 Rn.10474 
                 Pom121 
                 Nuclear pore membrane 
                 5′-AACGGAGUCCCUGCUGCAUUU 
                 712 
               
               
                   
                   
                   
                 glycoprotein 121 kD 
                 dTdT-3′ 
                   
               
               
                   
               
               
                 NM_053622.1 
                 Rn.10474 
                 Pom121 
                 Nuclear pore membrane 
                 5′-AACCAUGUCACCAGUCCAGUU 
                 713 
               
               
                   
                   
                   
                 glycoprotein 121 kD 
                 dTdT-3′ 
                   
               
               
                   
               
               
                 NM_053622.1 
                 Rn.10474 
                 Pom121 
                 Nuclear pore membrane 
                 5′-AAGCCUGUGUUUGGCUUUGGA 
                 714 
               
               
                   
                   
                   
                 glycoprotein 121 kD 
                 dTdT-3′ 
                   
               
               
                   
               
               
                 NM_131907.2 
                 Rn.5805 
                 Atp2c1 
                 ATPase, Ca++-sequestering 
                 5′-AACCATTATGGAAGAAGTACATT-3′ 
                 715 
               
               
                   
               
               
                 NM_031337.1 
                 Rn.22706 
                 Siar9 
                 Sialyltransferase 9 
                 5′-GGGUUAUUCUGAACAUGUUtt-3′ 
                 716 
               
               
                   
               
               
                 NM_031010.2 
                 Rn.11318 
                 Alox15 
                 Arachidonate 12-lipoxygenase 
                 5′-GCAACTGGATTTCTGTGAAGG-3′ 
                 717 
               
               
                   
               
               
                 NM_031010.2 
                 Rn.11318 
                 Alox15 
                 Arachidonate 12-lipoxygenase 
                 5′-GAAGCGGATTTCTTCCTTCTG-3′ 
                 718 
               
               
                   
               
               
                 NM_031556.1 
                 Rn.22518 
                 Cav 
                 Caveolin 
                 5′-AAGGAGATCGACCTGGTCAAC-3′ 
                 719 
               
               
                   
               
               
                 NM_031556.1 
                 Rn.22518 
                 Cav 
                 Caveolin 
                 5′-AAGGGACACACAGTTTTGACG-3′ 
                 720 
               
               
                   
               
               
                 XM_232106.2 
                 Rn.117974 
                 Dok-1 
                 Docking protein 1 (predicted) 
                 5′-GAACTACACAAATTCAGCCAGGCG 
                 721 
               
               
                   
                   
                   
                   
                 TATCATCCGGTGTTTCGTCCTTTCCAC 
                   
               
               
                   
                   
                   
                   
                 AAG-3′ 
                   
               
               
                   
               
               
                 NM_133307.1 
                 Rn.98279 
                 Prkcd 
                 Protein kinase C, delta 
                 5′-AAAAGGCAAATTCACAAACAGCCT 
                 722 
               
               
                   
                   
                   
                   
                 GTCTC-3′ 
                   
               
               
                   
               
               
                 NM_133307.1 
                 Rn.98279 
                 Prkcd 
                 Protein kinase C, delta 
                 5′-AAGTTCTCCGAAGTGTGAGAACCT 
                 723 
               
               
                   
                   
                   
                   
                 GTCTC-3′ 
                   
               
               
                   
               
               
                 NM_012637.1 
                 Rn.11317 
                 Ptpn1 
                 Protein tyrosine phosphatase, 
                 5′-AAGCTGACACTGATCTCTGAA-3′ 
                 724 
               
               
                   
                   
                   
                 non-receptor type 1 
                   
                   
               
               
                   
               
               
                 NM_017212 
                 Rn.2455 
                 Mapt 
                 Microtubule-associated protein 
                 5′-CCAGGAGTTTGACACAATG-3′ 
                 725 
               
               
                   
                   
                   
                 tau 
                   
                   
               
               
                   
               
               
                 NM_013156 
                 Rn.1294 
                 CTSL 
                 Cathepsin L 
                 5′-GGACAGAUGUUCCUUAAGATT-3′ 
                 726 
               
               
                   
               
               
                 NM_012576.1 
                 Rn.90070 
                 Nr3c1 
                 Nuclear receptor subfamily 3, 
                 5′-GGCCAAGGGAGGGGGAGCGTA-3′ 
                 727 
               
               
                   
                   
                   
                 group C, member 1 
                   
                   
               
               
                   
               
               
                 NM_013131.1 
                 Rn.9678 
                 Nr3c2 
                 Nuclear receptor subfamily 3, 
                 5′-GGCGCTGGAGTCAAGTGTCTC-3′ 
                 728 
               
               
                   
                   
                   
                 group C, member 2 
                   
                   
               
               
                   
               
               
                 NM_031659 
                 Rn.10039 
                 Tgm1 
                 Transglutaminase 1 
                 5′-AUGCAGCUGGAGAUGGCACdTdT-3′ 
                 729 
               
               
                   
               
               
                 NM_019275 
                 Rn.9774 
                 Madh4 
                 MAD homolog 4 
                 5′-AAUACACCGACAAGCAAUGACdTdT-3′ 
                 730 
               
               
                   
               
               
                 NM_031132 
                 Rn.9954 
                 Tgfbr2 
                 Transforming growth factor, 
                 5′-AAAGUCGGUUAACAGCGAUCUdTdT-3′ 
                 731 
               
               
                   
                   
                   
                 beta receptor II 
                   
                   
               
               
                   
               
               
                 NM_144741 
                 Rn.16746 
                 Retn 
                 Resistin 
                 5′-CCTTTCATTTCTCCTCCTT-3′ 
                 732 
               
               
                   
               
               
                 NM_144741 
                 Rn.16746 
                 Retn 
                 Resistin 
                 5′-AGCTGCTCCTGTGGCTCTG-3′ 
                 733 
               
               
                   
               
               
                 NM_144741 
                 Rn.16746 
                 Retn 
                 Resistin 
                 5′-GCCTCCTGCCCAGAAGGCA-3′ 
                 734 
               
               
                   
               
               
                 NM_012614 
                 Rn.9714 
                 Npy 
                 Neuropeptide Y 
                 5′-UGAGAGAAAGCACAGAAA-3′ 
                 735 
               
               
                   
               
               
                 NM_021597 
                 Rn.35512 
                 Eif2c2 
                 GERp95 
                 5′-UGGACAUCCCCAAAAUUGA-3′ 
                 736 
               
               
                   
               
               
                 NM_012547 
                 Rn.87299 
                 Drd2 
                 Dopamine receptor 2 
                 5′-CCCCAUCAUCUACACCACA-3′ 
                 737 
               
               
                   
               
            
           
         
       
     
     APPENDIX (B) 
     U.S. Pat. No. 6,207,646 B1 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Oligonucleotide Stimulation of Mouse B Cells 
               
            
           
           
               
               
               
            
               
                 ODN 
                   
                 Stimulation Index′ 
               
            
           
           
               
               
               
               
            
               
                  Production 
                 Sequence (5′ to 3′) 
                   3 H Uridine 
                 IgM 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 1 
                 (SEQ ID NO: 1) 
                 GCTAGA CG TTAG CGT   
                  6.1 ± 0.8 
                 17.9 ± 3.6  
               
               
                   
               
               
                 1a 
                 (SEQ ID NO: 2) 
                 ......T..... .. . 
                  1.2 ± 0.2 
                  1.7 ± 0.5 
               
               
                   
               
               
                 1b 
                 (SEQ ID NO: 3) 
                 ......Z..... .. . 
                  1.2 ± 0.1 
                  1.8 ± 0.0 
               
               
                   
               
               
                 1c 
                 (SEQ ID NO: 4) 
                 ...... .. ....Z.. 
                 10.3 ± 4.4  
                  9.5 ± 1.8 
               
               
                   
               
               
                 1d 
                 (SEQ ID NO: 5) 
                 ..AT... .. ..GAGC. 
                 13.0 ± 2.3  
                 18.3 ± 7.5  
               
               
                   
               
               
                 2 
                 (SEQ ID NO: 6) 
                 ATGGAAGGTCCAG CG TTCTC 
                  2.9 ± 0.2 
                 13.6 ± 2.0  
               
               
                   
               
               
                 2a 
                 (SEQ ID NO: 7) 
                 .. C. .CTC. .G .. .. ..... 
                  7.7 ± 0.8 
                 24.2 ± 3.2  
               
               
                   
               
               
                 2b 
                 (SEQ ID NO: 8) 
                 ..Z..CTC.ZG..Z...... 
                  1.6 ± 0.5 
                  2.8 ± 2.2 
               
               
                   
               
               
                 2c 
                 (SEQ ID NO: 9) 
                 ..Z..CTC. .G .. .. ..... 
                  3.1 ± 0.6 
                  7.3 ± 1.4 
               
               
                   
               
               
                 2d 
                 (SEQ ID NO: 10) 
                 .. C. .CTC. .G .. .. ..Z.. 
                  7.4 ± 1.4 
                 27.7 ± 5.4  
               
               
                   
               
               
                 2e 
                 (SEQ ID NO: 11) 
                 ............A .. ..... 
                  5.6 ± 2.0 
                 ND 
               
               
                   
               
               
                 3D 
                 (SEQ ID NO: 12) 
                 GAGAA CG CTGGACCTTCCAT 
                  4.9 ± 0.5 
                 19.9 ± 3.6  
               
               
                   
               
               
                 3Da 
                 (SEQ ID NO: 13) 
                 ..... .. .. C. ......... 
                  6.6 ± 1.5 
                 33.9 ± 6.8  
               
               
                   
               
               
                 3Db 
                 (SEQ ID NO: 14) 
                 ..... .. .. C. ..... .G .. 
                 10.1 ± 2.8  
                 25.4 ± 0.8  
               
               
                   
               
               
                 3Dc 
                 (SEQ ID NO: 15) 
                 ...C.A.............. 
                  1.0 ± 0.1 
                  1.2 ± 0.5 
               
               
                   
               
               
                 3Dd 
                 (SEQ ID NO: 17) 
                 .....Z.............. 
                  1.2 ± 0.2 
                  1.0 ± 0.4 
               
               
                   
               
               
                 3De 
                 (SEQ ID NO: 12) 
                 ..... .. ......Z...... 
                  4.4 ± 1.2 
                 18.8 ± 4.4  
               
               
                   
               
               
                 3Df 
                 (SEQ ID NO: 18) 
                 ..... .. A............ 
                  1.6 ± 0.1 
                  7.7 ± 0.4 
               
               
                   
               
               
                 3Dg 
                 (SEQ ID NO: 19) 
                 ..... .. ..CC.G.ACTG.. 
                  6.1 ± 1.5 
                 18.6 ± 1.5  
               
               
                   
               
               
                 3M 
                 (SEQ ID NO: 22) 
                 TCCATGT CG GTCCTGATGCT 
                  4.1 ± 0.2 
                 23.2 ± 4.9  
               
               
                   
               
               
                 3Ma 
                 (SEQ ID NO: 21) 
                 ......CT............ 
                  0.9 ± 0.1 
                  1.8 ± 0.5 
               
               
                   
               
               
                 3Mb 
                 (SEQ ID NO: 22) 
                 .......Z............ 
                  1.3 ± 0.3 
                  1.5 ± 0.6 
               
               
                   
               
               
                 3Mc 
                 (SEQ ID NO: 23) 
                 ....... .. ..Z........ 
                  5.4 ± 1.5 
                  8.5 ± 2.6 
               
               
                   
               
               
                 3Md 
                 (SEQ ID NO: 24) 
                 ......A .. T.......... 
                 17.2 ± 9.4  
                 ND 
               
               
                   
               
               
                 3Me 
                 (SEQ ID NO: 741) 
                 ....... .. ......C..A. 
                  3.6 ± 0.2 
                 14.2 ± 5.2  
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 4 
                 TCAACGTT (SEQ ID NO: 
                  6.1 ± 1.4 
                 19.2 ± 5.2  
               
               
                   
                 26) 
                   
                   
               
               
                   
               
               
                 4a 
                 ....GC.. (SEQ ID NO: 
                  1.1 ± 0.2 
                  1.5 ± 1.1 
               
               
                   
                 27) 
                   
                   
               
               
                   
               
               
                 4b 
                 ...G CG C. (SEQ ID NO: 
                  4.5 ± 0.2 
                  9.6 ± 3.4 
               
               
                   
                 28) 
                   
                   
               
               
                   
               
               
                 4c 
                 ...T CG A. (SEQ ID NO: 
                  2.7 ± 1.0 
                 ND 
               
               
                   
                 2306) 
                   
                   
               
               
                   
               
               
                 4d 
                 ..TT .. AA (SEQ ID NO: 
                  1.3 ± 0.2 
                 ND 
               
               
                   
                 30) 
                   
                   
               
               
                   
               
               
                 4e 
                 .... .. .. (SEQ ID NO: 
                  1.3 ± 0.2 
                 1.1 ± 0.5 
               
               
                   
                 31) 
                   
                   
               
               
                   
               
               
                 4f 
                 C... .. .. (SEQ ID NO: 
                  3.9 ± 1.4 
                 ND 
               
               
                   
                 32) 
                   
                   
               
               
                   
               
               
                 4g 
                 --.. .. ..CT (SEQ ID 
                  1.4 ± 0.3 
                 ND 
               
               
                   
                 NO: 2307) 
                   
                   
               
               
                   
               
               
                 4h 
                 .... .. .C (SEQ ID NO: 
                  1.2 ± 0.2 
                 ND 
               
               
                   
                 34) 
                   
                   
               
               
                   
               
               
                 LPS 
                   
                  7.8 ± 2.8 
                 4.8 ± 1.0 
               
               
                   
               
               
                 ′Stimulation indexes are the means and std. dev. derived from at least 3 separate experiments, and are compared to wells cultured with no added ODN. 
               
               
                 ND = not done. 
               
               
                 CpG dinucleotides are underlined. 
               
               
                 Dots indicate identity; dashes indicate deletion. 
               
               
                 Z indicates 5 methyl cytonine. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Identification of the optimal CpG motif for Nurine IL-6 production and B cell activation. 
               
            
           
           
               
               
               
            
               
                   
                 IL-6 (pg/ml) a   
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 ODN 
                   
                   
                   
                 SPLENIC 
                   
                   
               
               
                 (ng/ml) C   
                 SEQUENCE (5′-3′) 
                   
                 CH12.LX 
                 B CELL 
                 SI b   
                 IgM 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 512 
                 (SEQ ID No: 22) 
                 TCCATGT CG GTCCTGATGCT 
                 1300 ± 106 
                  627 ± 43 
                  5.8 ± 0.3 
                  7315 ± 1324 
               
               
                   
               
               
                 1637 
                 (SEQ ID No: 43) 
                 ......C .. ........... 
                  136 ± 27 
                   46 ± 6 
                  1.7 ± 0.2 
                   770 ± 72 
               
               
                   
               
               
                 1615 
                 (SEQ ID No: 44) 
                 ......G .. ........... 
                 1201 ± 155 
                  850 ± 202 
                  3.7 ± 0.3 
                  3212 ± 617 
               
               
                   
               
               
                 1614 
                 (SEQ ID No: 45) 
                 ......A .. ........... 
                 1533 ± 321 
                 1812 ± 103 
                 10.8 ± 0.6  
                  7558 ± 414 
               
               
                   
               
               
                 1636 
                 (SEQ ID No: 46) 
                 ....... .. A.......... 
                 1181 ± 76  
                  947 ± 132 
                  5.4 ± 0.4 
                  3983 ± 485 
               
               
                   
               
               
                 1634 
                 (SEQ ID No: 47) 
                 ....... .. C.......... 
                 1049 ± 223 
                 1671 ± 175 
                  9.2 ± 0.9 
                  6256 ± 261 
               
               
                   
               
               
                 1619 
                 (SEQ ID No: 48) 
                 ....... .. T.......... 
                 1555 ± 304 
                 2908 ± 129 
                 12.5 ± 1.0  
                  8243 ± 698 
               
               
                   
               
               
                 1618 
                 (SEQ ID No: 24) 
                 ......A .. T.......... 
                 2109 ± 291 
                 2596 ± 166 
                 12.9 ± 0.7  
                 10425 ± 674  
               
               
                   
               
               
                 1639 
                 (SEQ ID No: 49) 
                 .....AA .. T.......... 
                 1827 ± 83  
                 2012 ± 132 
                 11.5 ± 0.4  
                  9439 ± 103 
               
               
                   
               
               
                 1707 
                 (SEQ ID No: 50) 
                 ......A .. TC......... 
                 ND 
                 1147 ± 175 
                  4.0 ± 0.2 
                  3534 ± 217 
               
               
                   
               
               
                 1708 
                 (SEQ ID No: 51) 
                 .....CA .. TG......... 
                 ND 
                 59 ± 3 
                  1.5 ± 0.1 
                   466 ± 109 
               
               
                   
               
               
                 Dots indicate identity; 
               
               
                 CpG dinucleotides are underlined; 
               
               
                 ND = not done. 
               
               
                 The experiment was done at least three times with similar results. The level of IL-6 of unstimulated control cultures of both CH.2.LX and splenic B cells was ≤ 10 pg/ml. The IgM level of unstimulated culture was 547 ± 82 ng/ml. CpG dinucleotides are underlined and dots indicate identity. 
               
               
                   b [ 3 H] Uridine uptake was indicated as a fold increase (SI: stimulation index) from unstimulated control (2322.67 ± 213.68 cpm). Cells were stimulated with 20 μM of various CpG O-QDN. 
               
               
                 Data present the mean ± SD of triplicates 
               
               
                 Measured by ELISA. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Induction of Murine IL-6 secretion by CpG motifs 
               
               
                 in bacterial DNA or oligonucleotides. 
               
            
           
           
               
               
            
               
                 Treatment 
                 IL-6 (pg/ml) 
               
               
                   
               
               
                 calf thymus DNA 
                 ≤10 
               
               
                   
               
               
                 calf thymus DNA + DNase 
                 ≤10 
               
               
                   
               
               
                   E. coli  DNA 
                 1169.5 ± 94.1  
               
               
                   
               
               
                   E. coli  DNA + DNase 
                 ≤10 
               
               
                   
               
               
                 CpG methylated  E. coli  DNA 
                 ≤10 
               
               
                   
               
               
                 LPS 
                 280.1 ± 17.1 
               
               
                   
               
               
                 Media (no DNA) 
                 ≤10 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 ODN 
                 5a 
                 SEQ. 
                 ATGGACTCTCCAGCGTTCTC 
                 1096.4 ± 372.0 
               
               
                   
                   
                 ID 
                   
                   
               
               
                   
                   
                 No: 35 
                   
                   
               
               
                   
                 5b 
                 SEQ. 
                 .....AGG....A .. ..... 
                 1124.5 ± 126.2 
               
               
                   
                   
                 ID 
                   
                   
               
               
                   
                   
                 No:  
                   
                   
               
               
                   
                   
                 740 
                   
                   
               
               
                   
                 5c 
                 SEQ. 
                 .. C. ..... .G .. .. ..... 
                 1783.0 ± 189.5 
               
               
                   
                   
                 ID 
                   
                   
               
               
                   
                   
                 No: 7 
                   
                   
               
               
                   
                 5d 
                 SEQ. 
                 .... AGG..C..T...... 
                 ≤10 
               
               
                   
                   
                 ID 
                   
                   
               
               
                   
                   
                 No:  
                   
                   
               
               
                   
                   
                 4742 
                   
                   
               
               
                   
                 5e 
                 SEQ. 
                 .. C. ...... G ..Z...... 
                  851.1 ± 114.4 
               
               
                   
                   
                 ID 
                   
                   
               
               
                   
                   
                 No: 8 
                   
                   
               
               
                   
                 5f 
                 SEQ. 
                 ..Z......ZG..Z...... 
                 ≤10 
               
               
                   
                   
                 ID 
                   
                   
               
               
                   
                   
                 No: 9 
                   
                   
               
               
                   
                 5g 
                 SEQ. 
                 .. C. ..... .G .. .. ..Z.. 
                 1862.3 ± 87.26 
               
               
                   
                   
                 ID 
                   
                   
               
               
                   
                   
                 No: 10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Induction of human PBMC cytokine secrtetion by CpG oligos 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 ODN 
                 Sequence (5′-3′) 
                 IL-6 1   
                 TNF-α 1   
                 IFN-γ 1   
                 GM-CSF 
                 IL-12 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 512 
                 TCCATGT CG GTCCTGATGCT 
                 500 
                 140 
                 15.6 
                 70 
                 250 
               
               
                 SEQ ID NO: 22 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1637 
                 ......C .. ........... 
                 550 
                 16 
                 7.8 
                 15.6 
                 35 
               
               
                 SEQ ID NO: 43 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1615 
                 ......G .. ........... 
                 600 
                 145 
                 7.8 
                 45 
                 250 
               
               
                 SEQ ID NO: 44 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1645 
                 ......A............. 
                 550 
                 31 
                 0 
                 50 
                 250 
               
               
                 (SEQ ID NO: 45) 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1636 
                 ....... .. A.......... 
                 325 
                 250 
                 35 
                 40 
                 0 
               
               
                 SEQ ID NO: 46 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1634 
                 ....... .. C.......... 
                 300 
                 400 
                 40 
                 85 
                 200 
               
               
                 SEQ ID NO: 47 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1619 
                 ....... .. T.......... 
                 275 
                 450 
                 200 
                 80 
                 ≥500 
               
               
                 SEQ ID NO: 48 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1618 
                 ......A .. T.......... 
                 300 
                 60 
                 15.6 
                 15.6 
                 62 
               
               
                 SEQ ID NO: 24 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1639 
                 .....AA .. T.......... 
                 625 
                 220 
                 15.6 
                 40 
                 60 
               
               
                 SEQ ID NO: 49 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1707 
                 ......A .. TC......... 
                 300 
                 70 
                 17 
                 0 
                 0 
               
               
                 SEQ ID NO: 50 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1708 
                 .....CA .. TG......... 
                 270 
                 10 
                 17 
                 0 
                 0 
               
               
                 SEQ ID NO: 51 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Induction of NK Activity by DNA Containing CpG Motifs but not 
               
               
                 by Non-CpG DNA 
               
            
           
           
               
               
            
               
                   
                 LU/10 6   
               
            
           
           
               
               
               
               
               
            
               
                   
                 DNA or Cytokine Added 
                   
                 Mouse Cells 
                 Human cells 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Expt. 1 
                 None 
                   
                 0.00 
                 0.00 
               
               
                   
                 IL-2 
                   
                 16.68 
                 15.82 
               
               
                   
                   E. Coli , DNA 
                   
                 7.23 
                 5.05 
               
               
                   
                 Calf thymus DNA 
                   
                 0.00 
                 0.00 
               
               
                   
               
               
                 Expt. 2 
                 None 
                   
                 0.00 
                 3.28 
               
               
                   
                 1585 ggGGGGTCAA CG TTGAGGGGGGgggg 
                 (SEQ ID No. 743) 
                 7.38 
                 17.98 
               
               
                   
                 1629 ----------gtc------------- 
                 (SEQ ID No. 744) 
                 0.00 
                 4.4 
               
               
                   
               
               
                 Expt. 3 
                 None 
                   
                 0.00 
                   
               
               
                   
                 1613 GCTAGA CG TTAGTGT 
                 (SEQ ID No. 54) 
                 5.22 
                   
               
               
                   
                 1769 -------Z------- 
                 (SEQ ID No. 745) 
                 0.02 
                 ND 
               
               
                   
                 1619 TCCATGT CG TTCCTGATGCT 
                 (SEQ ID No: 48) 
                 3.35 
                   
               
               
                   
                 1765 -------Z------------ 
                 (SEQ ID No. 56) 
                 0.11 
               
               
                   
               
            
           
         
       
     
                     TABLE 10                  Different CpG motifs stimulate optima       lmurine B cell and NK activation                                     B cell   NK       ODM   Sequence   activation 1     activation 2                                       1668   TCCATGA CG TTCCTGATGCT   42,849   2.52           (SEQ. ID. NO: 24)                       1758   TCTCCCAG CG TG CG CCAT   1,747   6.66           (SEQ. ID. NO. 59)                       NONE       367   0.00               CpG dinucleotides are underlined; oligonucleotides were synthesized with phosphorothioate modified backbones to improve their nuclease resistance.         1 Measured by  3 H thymidine incorporation after 48 hr culture with oligodeoxynucleotides at a 200 nM concentration as described in Example 1.         2 Measured in lytic units.            
U.S. Pat. No. 6,214,806 B1
 
     
       
         
           
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 ODN Number 
                 cells/ml × 10 4   
                 PMN/ml × 10 3   
                 % PMN 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 expt 1, C3H/BFeJ mice 
               
            
           
           
               
               
               
               
            
               
                 1908 
                 61.4 ± 15.9 
                 59.2 ± 15.7 
                 95.8 ± 0.95 
               
               
                 1760 
                 27.8 ± 3.5* 
                 25.8 ± 3.0* 
                 93.3 ± 2.3 
               
               
                 1631 
                 47.6 ± 11.1 
                 46.1 ± 10.7 
                 96.8 ± 1.11 
               
               
                 1835 
                 43.8 ± 7.1 
                 44.4 ± 7.1 
                 96.8 ± 0.75 
               
               
                 1759 
                 71.0 ± 19.8 
                 67.7 ± 20.4 
                 96.8 ± 2.6 
               
               
                 1826 
                 39.8 ± 7.8 
                 38.3 ± 7.9 
                 93.5 ± 1.4 
               
               
                 None (saline) 
                 71.0 ± 7.4 
                 69.3 ± 6.9 
                 97.8 ± 1.3 
               
            
           
           
               
            
               
                 expt 2, C57 Bl/6 mice 
               
            
           
           
               
               
               
               
            
               
                 1908 
                 18.0 ± 2.6 
                 16.6 ± 2.7 
                 91.2 ± 3.7 
               
               
                 1760 
                 10.2 ± 2.3* 
                  8.6 ± 2.1* 
                 82.0 ± 3.0 
               
               
                 1585 
                 11.0 ± 2.2* 
                  9.5 ± 2.2* 
                 84.6 ± 2.9 
               
               
                 2010 
                 14.1 ± 2.1 
                 11.8 ± 1.9 
                 83.4 ± 2.1 
               
               
                 None (saline) 
                 17.9 ± 3.4 
                 16.9 ± 2.1 
                   
               
            
           
           
               
            
               
                 expt 3 
               
            
           
           
               
               
               
               
            
               
                 1908 
                 19.0 ± 2.5 
                 16.9 ± 2.1 
                 89.4 ± 1.4 
               
               
                 1760 
                  9.1 ± 0.8* 
                  7.7 ± 0.7* 
                 84.6 ± 0.5 
               
               
                 1972 
                 15.3 ± 1.6 
                 13.5 ± 1.4 
                 84.2 ± 1.2 
               
               
                 2001 
                 13.0 ± 1.6* 
                 11.8 ± 1.6 
                 90.4 ± 2.2 
               
               
                   
               
               
                 *P &gt; 0.05, Mean-Whitney U test 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Induction Of NK Activity By CpG Oligodeoxynucleotides (ODN) 
               
            
           
           
               
               
               
            
               
                   
                 % YAC-1 Specific Lysis* 
                 % 2C11 Specific Lysis 
               
               
                   
                 Effector:Target 
                 Effector:Target 
               
            
           
           
               
               
               
               
               
            
               
                 ODN 
                 50:1 
                 100:1 
                 50:1 
                 100:1 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 None 
                 −1.1 
                 −1.4 
                 15.3 
                 16.6 
               
               
                 1 
                 16.1 
                 24.5 
                 38.7 
                 47.2 
               
               
                 3Dd 
                 17.1 
                 27.0 
                 37.0 
                 40.0 
               
               
                 non-CpG ODN 
                 −1.6 
                 −1.7 
                 14.8 
                 15.4 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Induction of NK Activity by DNA 
               
               
                 Containing CpG Motifs but not by Non-CpG DNA 
               
            
           
           
               
               
            
               
                   
                 LU/10 5   
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Mouse 
                 Human 
               
               
                   
                 DNA or Cytokine Added 
                 Cells 
                 Cells 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 Expt. 1 
                 None 
                 0.00 
                 0.00 
               
               
                   
                 IL-2 
                 16.68 
                 15.82 
               
               
                   
                   E. Coli  DNA 
                 7.23 
                 5.05 
               
               
                   
                 Calf thymus DNA 
                 0.00 
                 0.00 
               
               
                   
               
               
                 Expt. 2 
                 None 
                 0.00 
                 3.28 
               
               
                   
                 1585 ggGGTCAA CG TTGACgggg 
                 7.38 
                 17.98 
               
               
                   
                 (SEQ ID NO: 746) 
                   
                   
               
               
                   
                 1629 --------gtc-------- 
                 0.00 
                 4.4 
               
               
                   
                 (SEQ ID NO: 747) 
                   
                   
               
               
                   
               
               
                 Expt. 3 
                 None 
                 0.00 
                   
               
               
                   
                 1613 GCTAGA CG TTAGTGT 
                 5.22 
                   
               
               
                   
                 (SEQ ID NO: 54) 
                   
                   
               
               
                   
                 1769 -------X----- 
                 0.02 
                 ND 
               
               
                   
                 (SEQ ID NO: 745) 
                   
                   
               
               
                   
                 1619 TCCATGT CG TTCCTGATGCT 
                 3.35 
                   
               
               
                   
                 (SEQ ID NO: 48) 
                   
                   
               
               
                   
                 1765 --------X----------- 
                 0.11 
                   
               
               
                   
                 (SEQ ID NO: 748) 
               
               
                   
               
               
                 CpG dinucleotides in ODN sequences are indicated by underlining; 
               
               
                 X indicates methylcytosine. 
               
               
                 Lower case letters indicate nuclease resistant phosphorothioate modified internucleotide linkages which, in titration experiments, were more than 20 times as potent as non-modified ODN, depending on the flanking bases. 
               
               
                 Poly G ends (g) were used in some ODN, because they significantly increase the level of ODN uptake. 
               
               
                 Dashes indicate some bases are identical to those in the directly preceding sequence, with the exception of changes noted. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 ODN induction of NK Lytic Activity (LU) 
               
            
           
           
               
               
               
               
            
               
                   
                 ODN 
                 Sequence (5′-3′) 
                 LU 
               
               
                   
                   
               
               
                   
                 None 
                   
                 0.01 
               
               
                   
                   
               
               
                   
                 1754 
                 ACCATGGACGATCTGTTTCCCCTC 
                 0.02 
               
               
                   
                   
                 (SEQ ID NO: 58) 
                   
               
               
                   
                   
               
               
                   
                 1758 
                 TCTCCCAGCGTGCGCCAT 
                 0.05 
               
               
                   
                   
                 (SEQ ID NO: 59) 
                   
               
               
                   
                   
               
               
                   
                 1761 
                 TACCGCGTGCGACCCTCT 
                 0.05 
               
               
                   
                   
                 (SEQ ID NO: 60) 
                   
               
               
                   
                   
               
               
                   
                 1776 
                 ACCATGGACGAACTGTTTCCCCTC 
                 0.03 
               
               
                   
                   
                 (SEQ ID NO: 61) 
                   
               
               
                   
                   
               
               
                   
                 1777 
                 ACCATGGACGAGCTGTTTCCCCTC 
                 0.05 
               
               
                   
                   
                 (SEQ ID NO: 62) 
                   
               
               
                   
                   
               
               
                   
                 1778 
                 ACCATGGACGACCTGTTTCCCCTC 
                 0.01 
               
               
                   
                   
                 (SEQ ID NO: 63) 
                   
               
               
                   
                   
               
               
                   
                 1779 
                 ACCATGGACGTACTGTTTCCCCTC 
                 0.02 
               
               
                   
                   
                 (SEQ ID NO: 64) 
                   
               
               
                   
                   
               
               
                   
                 1780 
                 ACCATGGACGGTCTGTTTCCCCTC 
                 0.29 
               
               
                   
                   
                 (SEQ ID NO: 65) 
                   
               
               
                   
                   
               
               
                   
                 1781 
                 ACCATGGACGTTCTGTTTCCCCTC 
                 0.38 
               
               
                   
                   
                 (SEQ ID NO: 66) 
                   
               
               
                   
                   
               
               
                   
                 1823 
                 GCATGACGTTGAGCT 
                 0.08 
               
               
                   
                   
                 (SEQ ID NO: 41) 
                   
               
               
                   
                   
               
               
                   
                 1824 
                 CACGTTGAGGGGCAT 
                 0.01 
               
               
                   
                   
                 (SEQ ID NO: 67) 
                   
               
               
                   
                   
               
               
                   
                 1825 
                 CTGCTGAGACTGGAG 
                 0.01 
               
               
                   
                   
                 (SEQ ID NO: 68) 
                   
               
               
                   
                   
               
               
                   
                 1828 
                 TCAGCGTGCGCC 
                 0.01 
               
               
                   
                   
                 (SEQ ID NO: 69) 
                   
               
               
                   
                   
               
               
                   
                 1829 
                 ATGACGTTCCTGACGTT 
                 0.42 
               
               
                   
                   
                 (SEQ ID NO: 70) 
                   
               
               
                   
                   
               
               
                   
                 1830 2   
                 RANDOM SEQUENCE 
                 0.25 
               
               
                   
                   
               
               
                   
                 1834 
                 TCTCCCAGCGGGCGCAT 
                 0.00 
               
               
                   
                   
                 (SEQ ID NO: 71) 
                   
               
               
                   
                   
               
               
                   
                 1836 
                 TCTCCCAGCGCGCGCCAT 
                 0.46 
               
               
                   
                   
                 (SEQ ID NO: 72) 
                   
               
               
                   
                   
               
               
                   
                 1840 
                 TCCATGTCGTTCCTGTCGTT 
                 2.70 
               
               
                   
                   
                 (SEQ ID NO: 73) 
                   
               
               
                   
                   
               
               
                   
                 1841 
                 TCCATAGCGTTCCTAGCGTT 
                 1.45 
               
               
                   
                   
                 (SEQ ID NO: 74) 
                   
               
               
                   
                   
               
               
                   
                 1842 
                 TCGTCGCTGTCTCCGCTTCTT 
                 0.06 
               
               
                   
                   
                 (SEQ ID NO: 75) 
                   
               
               
                   
                   
               
               
                   
                 1851 
                 TCCTGACGTTCCTGACGTT 
                 2.32 
               
               
                   
                   
                 (SEQ ID NO: 76) 
               
               
                   
                   
               
               
                   
                   1 Lytic units (LU) were measured as described (8). 
               
               
                   
                 Briefly, PBMC were collected from normal donors and spun over Ficoll, then cultured with or without the indicated ODN (which were added to cultures at 6 μg/ml) for 24 hr. Then their ability to lyse  51 Cr-labeled K562 cells was determined. The results shown are typical of those obtained with several different normal human donors. 
               
               
                   
                   2 This oligo mixture contained a random selection of all 4 bases at each position. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Induction of NK LU by Phoshorothioate Cp 
               
               
                 GODN with Good Motifs 
               
            
           
           
               
               
               
               
               
            
               
                 ODN 1   
                 sequence (5′-3′) 
                 expt. 1 
                 expt. 2 
                 expt. 3 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 None 
                   
                 0.00 
                 1.26 
                 0.46 
               
               
                   
               
               
                 1840 
                 TCCATGT CG TTCCTGT CG TT (SEQ ID NO: 73) 
                 2.33 
                 ND 
                 ND 
               
               
                   
               
               
                 1960 
                 TCCTGT CG TTCCTGT CG TT (SEQ ID NO: 77) 
                 ND 
                 0.48 
                 8.99 
               
               
                   
               
               
                 1961 
                 TCCATGT CG TTTTTGT CG TT (SEQ ID NO: 78) 
                 4.03 
                 1.23 
                 5.08 
               
               
                   
               
               
                 1962 
                 TCCTGT CG TTCCTTGT CG TT (SEQ ID NO: 79) 
                 ND 
                 1.60 
                 5.74 
               
               
                   
               
               
                 1963 
                 TCCTTGT CG TTCCTGT CG TT (SEQ ID NO: 80) 
                 3.42 
                 ND 
                 ND 
               
               
                   
               
               
                 1965 
                 TCCTGT CG TTTTTTGT CG TT (SEQ ID NO: 81) 
                 0.46 
                 0.42 
                 3.48 
               
               
                   
               
               
                 1966 
                 T CG T CG CTGTCTC CG CTTCTT (SEQ ID NO: 75) 
                 2.62 
                 ND 
                 ND 
               
               
                   
               
               
                 1967 
                 T CG T CG CTGTCTGCCCTTCTT (SEQ ID NO: 82) 
                 5.82 
                 1.64 
                 8.32 
               
               
                   
               
               
                 1968 
                 T CG T CG CTGTTGT CG TTTCTT (SEQ ID NO: 83) 
                 3.77 
                 5.26 
                 6.12 
               
               
                   
               
               
                 1979 2   
                 TCCATGTZGTTCCTGTZGTT (SEQ ID NO: 84) 
                 1.32 
                 ND 
                 ND 
               
               
                   
               
               
                 1982 
                 TCCAGGACTTCTCTCAGGTT (SEQ ID NO: 85) 
                 0.05 
                 ND 
                 0.98 
               
               
                   
               
               
                 1990 
                 TCCATG CG TG CG TG CG TTTT (SEQ ID NO: 86) 
                 2.10 
                 ND 
                 ND 
               
               
                   
               
               
                 1991 
                 TCCATG CG TTG CG TTG CG TT (SEQ ID NO: 87) 
                 0.89 
                 ND 
                 ND 
               
               
                   
               
               
                 2002 
                 TCCA CG ACGTTTT CG A CG TT (SEQ ID NO: 88) 
                 4.02 
                 1.31 
                 9.79 
               
               
                   
               
               
                 2005 
                 TCGT CG TTGT CG TTGT CG TT (SEQ ID NO: 89) 
                 ND 
                 4.22 
                 12.75 
               
               
                   
               
               
                 2006 
                 TCGT CG TTTTGT CG TTTTGT CG TT (SEQ ID NO: 90) 
                 ND 
                 6.17 
                 12.82 
               
               
                   
               
               
                 2007 
                 TCGT CG TTGT CG TTTTGT CG TT (SEQ ID NO: 91) 
                 ND 
                 2.68 
                 9.66 
               
               
                   
               
               
                 2008 
                 G CG TG CG TTGT CG TTGT CG TT (SEQ ID NO: 92) 
                 ND 
                 1.37 
                 8.15 
               
               
                   
               
               
                 2010 
                 G CG G CG GG CG G CGCGCG CCC (SEQ ID NO: 93) 
                 ND 
                 0.01 
                 0.05 
               
               
                   
               
               
                 2012 
                 TGT CG TTTGT CG TTTGT CG TT (SEQ ID NO: 94) 
                 ND 
                 2.02 
                 11.61 
               
               
                   
               
               
                 2013 
                 TGT CG TTGT CG TTGT CG TTGT CG TT (SEQ ID NO: 95) 
                 ND 
                 0.56 
                 5.22 
               
               
                   
               
               
                 2014 
                 TGT CG TTGT CG TTGT CG TT (SEQ ID NO: 96) 
                 ND 
                 5.74 
                 10.89 
               
               
                   
               
               
                 2015 
                 T CG T CG TCGT CG TT (SEQ ID NO: 97) 
                 ND 
                 4.53 
                 10.13 
               
               
                   
               
               
                 2016 
                 TGT CG TTGT CG TT (SEQ ID NO: 98) 
                 ND 
                 6.54 
                 8.06 
               
               
                   
               
               
                   1 PBMC essentially as described herein. Results are representative of 6 separate experiments; each experiment represents a different donor. 
               
               
                   2 This is the methylated version of ODN 1840; 
               
               
                 Z = 5-methyl cytosine LU is lytic units; 
               
               
                 ND = not done; 
               
               
                 CpG dinucleotides are underlined for clarity. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Induction of human B cell proliferation by Phosphorothioate CpG ODN 
               
            
           
           
               
               
            
               
                   
                 Stimulation Index 1   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                   
                 expt. 
                   
                   
                   
                   
                   
               
               
                 DB 
                 sequence (5′-3′) 
                 1 
                 expt. 2 
                 expt. 3 
                 expt. 4 
                 expt. 5 
                 expt. 6 
               
               
                   
               
               
                 1840 
                 TCCATGT CG TTCCTGT CG TT (SEQ ID NO: 84) 
                 4 
                 ND 
                 ND 
                 ND 
                 ND 
                 34 
               
               
                   
               
               
                 1841 
                 TCCATAG CG TTCCTAG CG TT (SEQ ID NO: 99) 
                 3 
                 ND 
                 ND 
                 ND 
                 ND 
                 ND 
               
               
                   
               
               
                 1960 
                 TCCTGT CG TTCCTGT CG TT (SEQ ID NO: 77) 
                 ND 
                 2.0 
                 2.0 
                 3.6 
                 ND 
                 ND 
               
               
                   
               
               
                 1961 
                 TCCATGT CG TTTTTGT CG TT (SEQ ID NO: 78) 
                 2 
                 3.9 
                 1.9 
                 3.7 
                 ND 
                 37 
               
               
                   
               
               
                 1962 
                 TCCTGT CG TTCCTTGT CG TT (SEQ ID NO: 79) 
                 ND 
                 3.8 
                 1.9 
                 3.9 
                 5.4 
                 35 
               
               
                   
               
               
                 1963 
                 TCCTTGT CG TTCCTGT CG TT (SEQ ID NO: 80) 
                 3 
                 ND 
                 ND 
                 ND 
                 ND 
                 ND 
               
               
                   
               
               
                 1965 
                 TCCTGT CG TTTTTTGT CG TT (SEQ ID NO: 81) 
                 4 
                 3.7 
                 2.4 
                 4.7 
                 6.0 
                 43 
               
               
                   
               
               
                 1967 
                 T CG T CG CTGTCTGCCCTTCTT (SEQ ID NO: 82) 
                 ND 
                 4.4 
                 2.0 
                 4.5 
                 5.0 
                 36 
               
               
                   
               
               
                 1968 
                 T CG T CG CTGTTGT CG TTTCTT (SEQ ID NO: 83) 
                 ND 
                 4.0 
                 2.0 
                 4.9 
                 8.7 
                 38 
               
               
                   
               
               
                 1982 
                 TCCAGGACTTCTCTCAGGTT (SEQ ID NO: 85) 
                 3 
                 1.8 
                 1.3 
                 3.1 
                 3.2 
                 12 
               
               
                   
               
               
                 2002 
                 TCCA CG A CG TTTT CG A CG TT (SEQ ID NO: 88) 
                 ND 
                 2.7 
                 1.4 
                 4.4 
                 ND 
                 14 
               
               
                   
               
               
                 2005 
                 T CG T CG TTGT CG TTGT CG TT (SEQ ID NO: 89) 
                 5 
                 3.2 
                 1.2 
                 3.0 
                 7.9 
                 37 
               
               
                   
               
               
                 2006 
                 T CG T CG TTTTGT CG TTTTGT CG TT (SEQ ID NO: 90) 
                 4 
                 4.5 
                 2.2 
                 5.8 
                 8.3 
                 40 
               
               
                   
               
               
                 2007 
                 T CG T CG TTGT CG TTTTGT CG TT (SEQ ID NO: 91) 
                 3 
                 4.0 
                 4.2 
                 4.1 
                 ND 
                 22 
               
               
                   
               
               
                 2008 
                 G CG TG CG TTGT CG TTGT CG TT (SEQ ID NO: 92) 
                 ND 
                 3.0 
                 2.4 
                 1.6 
                 ND 
                 12 
               
               
                   
               
               
                 2010 
                 G CG G CG GG CG G CGCGCG CCC (SEQ ID NO: 93) 
                 ND 
                 1.6 
                 1.9 
                 3.2 
                 ND 
                 ND 
               
               
                   
               
               
                 2012 
                 TGT CG TTTGT CG TTTGT CG TT (SEQ ID NO: 94) 
                 2 
                 2.8 
                 0   
                 3.2 
                 ND 
                 33 
               
               
                   
               
               
                 2013 
                 TGT CG TTGT CG TTGT CG TTGT CG TT (SEQ ID NO: 95) 
                 3 
                 2.3 
                 3.1 
                 2.8 
                 ND 
                  7 
               
               
                   
               
               
                 2014 
                 TGT CG TTGT CG TTGT CG TT (SEQ ID NO: 96) 
                 3 
                 2.5 
                 4.0 
                 3.2 
                 6.7 
                 14 
               
               
                   
               
               
                 2015 
                 T CG T CG T CG T CG TT (SEQ ID NO: 97) 
                 5 
                 1.8 
                 2.6 
                 4.5 
                 9.4 
                  1 
               
               
                   
               
               
                 2016 
                 TGT CG TTGT CG TT (SEQ ID NO: 98) 
                 ND 
                 1.1 
                 1.7 
                 2.7 
                 7.3 
                  1 
               
               
                   
               
               
                   1 Cells = human spleen cells stored at −70° C. after surgical harvest or PBMC collected from normal donors and spun over Ficoll. Cells were cultured in 96 well U-bottom microtiter plates with or without the indicated ODN (which were added to cultures at 6 μml). N = 12 experiments. Cells were cultured for 4-7 days, pulsed with 1 μCi of  3 H thymidine for 18 hr before harvest and scintillation counting. Stimulation index = the ratio of cpm in wells without ODN to that in wells that had been stimulated throughout the culture period with the indicated ODN (there were no further additions of ODN after the cultures were set up). 
               
               
                 ND = not done 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Induction of human IL-12 
               
               
                 secretion by Phosphorothioate CpG ODN 
               
            
           
           
               
               
            
               
                   
                 IL-12 (pg/ml) 
               
            
           
           
               
               
               
               
            
               
                 ODN 1   
                 sequence (5′-3′) 
                 expt. 1 
                 expt. 2 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 None 
                   
                 0 
                 0 
               
               
                   
               
               
                 1962 
                 TCCTGTCGTTCCTTGTCGTT 
                 19 
                 0 
               
               
                   
                 (SEQ ID NO: 79) 
                   
                   
               
               
                   
               
               
                 1965 
                 TCCTGTCGTTTTTTGTCGTT 
                 36 
                 0 
               
               
                   
                 (SEQ ID NO: 81) 
                   
                   
               
               
                   
               
               
                 1967 
                 TCGTCGCTGTCTGCCCTTCTT 
                 41 
                 0 
               
               
                   
                 (SEQ ID NO: 82) 
                   
                   
               
               
                   
               
               
                 1968 
                 TCGTCGCTGTTGTCGTTTCTT 
                 24 
                 0 
               
               
                   
                 (SEQ ID NO: 83) 
                   
                   
               
               
                   
               
               
                 2005 
                 TCGTCGTTGTCGTTGTCGTT 
                 25 
                 0 
               
               
                   
                 (SEQ ID NO: 89) 
                   
                   
               
               
                   
               
               
                 2006 
                 TCGTCGTTTTGTCGTTTTGTCGTT 
                 29 
                 15 
               
               
                   
                 (SEQ ID NO: 90) 
                   
                   
               
               
                   
               
               
                 2014 
                 TGTCGTTGTCGTTGTCGTT 
                 28 
                 0 
               
               
                   
                 (SEQ ID NO: 96) 
                   
                   
               
               
                   
               
               
                 2015 
                 TCGTCGTCGTCGTT 
                 14 
                 0 
               
               
                   
                 (SEQ ID NO: 97) 
                   
                   
               
               
                   
               
               
                 2016 
                 TGTCGTTGTCGTT 
                 3 
                 0 
               
               
                   
                 (SEQ ID NO: 98) 
               
               
                   
               
               
                   1 PBMC were collected from normal donors and spun over Ficoll, then cultured at 10 6  cells/well in 96 well microtiter plates with or without the indicated ODN which were added to cultures at 6 μg/ml. Supernatants were collected at 24 hr and tested for IL-12 levels by ELISA as described in methods. A standard curve was run in each experiment, which represents a different donor. 
               
            
           
         
       
     
                     TABLE 8                  Different CpG motifs stimulate optimal murine       B cell and NK activation                                     B cell   NK       ODN   Sequence   activation 1     activation 2                                       1668   TCCATGA CG TTCCTGATGCT   42,849   2.52           (SEQ ID NO: 24)                       1758   TCTCCCAG CG TG CG CCAT   1,747   6.66           (SEQ ID NO: 59)                       NONE       367   0.00               CpG dinucleotides are underlined;       oligonucleotides were synthesized with phosphorothioate modified backbones to improve their nuclease resistance.         1 Measured by  3 H thymidine incorporation after 48 hr culture with oligodeoxy-nucleotides at a 200 nM concentration as described in Example 1.         2 Measured in lytic units.            
U.S. Pat. No. 6,218,371 B1
 
     
       
         
           
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 sequences 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 GCTAGA CG TTAG CGT   
                 (SEQ ID NO: 1) 
               
               
                   
               
               
                   
                 GCTAGATGTTAG CG T 
                 (SEQ ID NO: 2) 
               
               
                   
               
               
                   
                 GCTAGAZGTTAG CG T 
                 (SEQ ID NO: 3) 
               
               
                   
               
               
                   
                 GCTAGA CG TTAGZGT 
                 (SEQ ID NO: 4) 
               
               
                   
               
               
                   
                 GCATGA CG TTGAGCT 
                 (SEQ ID NO: 5) 
               
               
                   
               
               
                   
                 ATGGAAGGTCCAG CG TTCTC 
                 (SEQ ID NO: 6) 
               
               
                   
               
               
                   
                 AT CG ACTCT CG AG CG TTCTC 
                 (SEQ ID NO: 7) 
               
               
                   
               
               
                   
                 ATZ G ACTCTZGAGZ G TTCTC 
                 (SEQ ID NO: 8) 
               
               
                   
               
               
                   
                 ATZ G ACTCT CG AG CG TTCTC 
                 (SEQ ID NO: 9) 
               
               
                   
               
               
                   
                 AT CG ACTCT CG AG CG TTZTC 
                 (SEQ ID NO: 10) 
               
               
                   
               
               
                   
                 AT CG ACTCT CG AA CG TTCTC 
                 (SEQ ID NO: 11) 
               
               
                   
               
               
                   
                 GAGAA CG CTGGACCTTCCAT 
                 (SEQ ID NO: 12) 
               
               
                   
               
               
                   
                 GAGAA CG CT CG ACCTTCCAT 
                 (SEQ ID NO: 13) 
               
               
                   
               
               
                   
                 GAGAA CG CT CG ACCTT CG AT 
                 (SEQ ID NO: 14) 
               
               
                   
               
               
                   
                 GAGCA AG CTGGACCTTCCAT 
                 (SEQ ID NO: 15) 
               
               
                   
               
               
                   
                 GAGCAZ G CTGGACCTTCCAT 
                 (SEQ ID NO: 16) 
               
               
                   
               
               
                   
                 GAGAA CG CTGGACZTTCCAT 
                 (SEQ ID NO: 17) 
               
               
                   
               
               
                   
                 GAGAA CG ATGGACCTTCCAT 
                 (SEQ ID NO: 18) 
               
               
                   
               
               
                   
                 TCCATGTZ G TTCCTGATGCT 
                 (SEQ ID NO: 57) 
               
               
                   
               
               
                   
                 ACCATGGACGATCTGTTTCCCCTC 
                 (SEQ ID NO: 58) 
               
               
                   
               
               
                   
                 TCTCCCAGCGTGCGCCAT 
                 (SEQ ID NO: 59) 
               
               
                   
               
               
                   
                 TACCGCGTGCGACCCTCT 
                 (SEQ ID NO: 60) 
               
               
                   
               
               
                   
                 ACCATGGACGAACTGTTTCCCCTC 
                 (SEQ ID NO: 61) 
               
               
                   
               
               
                   
                 ACCATGGACGAGCTGTTTCCCCTC 
                 (SEQ ID NO: 62) 
               
               
                   
               
               
                   
                 ACCATGGACGACCTGTTTCCCCTC 
                 (SEQ ID NO: 63) 
               
               
                   
               
               
                   
                 ACCATGGACGTACTGTTTCCCCTC 
                 (SEQ ID NO: 64) 
               
               
                   
               
               
                   
                 ACCATGGACGGTCTGTTTCCCCTC 
                 (SEQ ID NO: 65) 
               
               
                   
               
               
                   
                 ACCATGGACGTTCTGTTTCCCCTC 
                 (SEQ ID NO: 66) 
               
               
                   
               
               
                   
                 CACGTTGAGGGGCAT 
                 (SEQ ID NO: 67) 
               
               
                   
               
               
                   
                 CTGCTGAGACTGGAG 
                 (SEQ ID NO: 68) 
               
               
                   
               
               
                   
                 TCAGCGTGCGCC 
                 (SEQ ID NO: 69) 
               
               
                   
               
               
                   
                 ATGACGTTCCTGACGTT 
                 (SEQ ID NO: 70) 
               
               
                   
               
               
                   
                 TCTCCCAGCGGGCGCAT 
                 (SEQ ID NO: 71) 
               
               
                   
               
               
                   
                 TCTCCCAGCGCGCGCCAT 
                 (SEQ ID NO: 72) 
               
               
                   
               
               
                   
                 TCCATGTCGTTCCTGTCGTT 
                 (SEQ ID NO: 73) 
               
               
                   
               
               
                   
                 TCCATAGCGTTCCTAGCGTT 
                 (SEQ ID NO: 74) 
               
               
                   
               
               
                   
                 TCGTCGCTGTCTCCGCTTCTT 
                 (SEQ ID NO: 75) 
               
               
                   
               
               
                   
                 TCCTGACGTTCCTGACGTT 
                 (SEQ ID NO: 76) 
               
               
                   
               
               
                   
                 TCCTGT CG TTCCTGT CG TT 
                 (SEQ ID NO: 77) 
               
               
                   
               
               
                   
                 TCCATGT CG TTTTTGT CG TT 
                 (SEQ ID NO: 78) 
               
               
                   
               
               
                   
                 TCCTGT CG TTCCTTGT CG TT 
                 (SEQ ID NO: 79) 
               
               
                   
               
               
                   
                 TCCTTGT CG TTCCTGT CG TT 
                 (SEQ ID NO: 80) 
               
               
                   
               
               
                   
                 TCCTGT CG TTTTTTGT CG TT 
                 (SEQ ID NO: 81) 
               
               
                   
               
               
                   
                 T CG T CG CTGTCTGCCCTTCTT 
                 (SEQ ID NO: 82) 
               
               
                   
               
               
                   
                 T CG T CG CTGTTGT CG TTTCTT 
                 (SEQ ID NO: 83) 
               
               
                   
               
               
                   
                 TCCATGTZGTTCCTGTZGTT 
                 (SEQ ID NO: 84) 
               
               
                   
               
               
                   
                 TCCAGGACTTCTCTCAGGTT 
                 (SEQ ID NO: 85) 
               
               
                   
               
               
                   
                 TCCATG CG TG CG TG CG TTTT 
                 (SEQ ID NO: 86) 
               
               
                   
               
               
                   
                 TCCATG CG TTG CG TTG CG TT 
                 (SEQ ID NO: 87) 
               
               
                   
               
               
                   
                 TCCA CG A CG TTTT CG A CG TT 
                 (SEQ ID NO: 88) 
               
               
                   
               
               
                   
                 T CG T CG TTGT CG TTGT CG TT 
                 (SEQ ID NO: 89) 
               
               
                   
               
               
                   
                 T CG T CG TTTTGT CG TTTTGT CG TT 
                 (SEQ ID NO: 90) 
               
               
                   
               
               
                   
                 T CG T CG TTGT CG TTTTGT CG TT 
                 (SEQ ID NO: 91) 
               
               
                   
               
               
                   
                 G CG TG CG TTGT CG TTGT CG TT 
                 (SEQ ID NO: 92) 
               
               
                   
               
               
                   
                 G CG G CG GG CG G CGCGCG CCC 
                 (SEQ ID NO: 93) 
               
               
                   
               
               
                   
                 TGT CG TTTGT CG TTTGT CG TT 
                 (SEQ ID NO: 94) 
               
               
                   
               
               
                   
                 TGT CG TTGT CG TTGT CG TTGT CG TT 
                 (SEQ ID NO: 95) 
               
               
                   
               
               
                   
                 TGT CG TTGT CG TTGT CG TT 
                 (SEQ ID NO: 96) 
               
               
                   
               
               
                   
                 T CG T CG T CG T CG TT 
                 (SEQ ID NO: 97) 
               
               
                   
               
               
                   
                 TGT CG TTGT CG TT 
                 (SEQ ID NO: 98) 
               
               
                   
               
               
                   
                 TCCATAG CG TTCCTAG CG TT 
                 (SEQ ID NO: 99) 
               
               
                   
               
               
                   
                 TCCATGACGTTCCTGACGTT 
                 (SEQ ID NO: 100) 
               
               
                   
               
               
                   
                 GTCG(T/C)T 
                 (SEQ ID NO: 101) 
               
               
                   
               
               
                   
                 TGTCG(T/C)T 
                 (SEQ ID NO: 102) 
               
               
                   
               
               
                   
                 TCCATGAGCTTCCTGAGTCT 
                 (SEQ ID NO: 103) 
               
               
                   
               
               
                   
                 TCTCCCAG CG TG CG CCAT 
                 (SEQ ID NO: 104) 
               
               
                   
               
               
                   
                 TCCATGACGTTCCTGACGTT 
                 (SEQ ID NO: 105) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Induction of human IL-12 secretion by 
               
               
                 Phosphorothioate CpG oliqonucleotide 
               
            
           
           
               
               
            
               
                   
                 IL-12 (pg/ml) 
               
            
           
           
               
               
               
               
            
               
                 ODN 1   
                 sequence (5′-3′) 
                 expt. 1 
                 expt. 2 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 None 
                   
                 0 
                 0 
               
               
                   
               
               
                 1962 
                 TCCTGTCGTTCCTTGTCGTT 
                 19 
                 0 
               
               
                   
                 (SEQ. ID NO: 79) 
                   
                   
               
               
                   
               
               
                 1965 
                 TCCTGTCGTTTTTTGTCGTT 
                 36 
                 0 
               
               
                   
                 (SEQ. ID NO: 81) 
                   
                   
               
               
                   
               
               
                 1967 
                 TCGTCGCTGTCTGCCCTTCTT 
                 41 
                 0 
               
               
                   
                 (SEQ. ID NO: 82) 
                   
                   
               
               
                   
               
               
                 1968 
                 TCGTCGCTGTTGTCGTTTCTT 
                 24 
                 0 
               
               
                   
                 (SEQ. ID NO: 83) 
                   
                   
               
               
                   
               
               
                 2005 
                 TCGTCGTTGTCGTTGTCGTT 
                 25 
                 0 
               
               
                   
                 (SEQ. ID NO: 89) 
                   
                   
               
               
                   
               
               
                 2006 
                 TCGTCGTTTTGTCGTTTTGTCGTT 
                 29 
                 15 
               
               
                   
                 (SEQ ID NO: 90) 
                   
                   
               
               
                   
               
               
                 2014 
                 TGTCGTTGTCGTTGTCGTT 
                 28 
                 0 
               
               
                   
                 (SEQ. ID NO: 96) 
                   
                   
               
               
                   
               
               
                 2015 
                 TCGTCGTCGTCGTT 
                 14 
                 0 
               
               
                   
                 (SEQ ID NO: 97) 
                   
                   
               
               
                   
               
               
                 2016 
                 TGTCGTTGTCGTT 
                 3 
                 0 
               
               
                   
                 (SEQ. ID NO: 98) 
               
               
                   
               
               
                   1 PBMC were collected from normal donors and spun over Ficoll, then cultured at 10 6  cells/well in 96 well microtiter plates with or without the indicated oligonucleotide which were added to cultures at μg/ml. Supernatants were collected at 24 hr and tested for IL-12 levels by ELISA as described in methods. A standard curve was run in each experiment, which represents a different donor. 
               
            
           
         
       
     
                                     TABLE 3                       CD86   CD40               Compound   (5 Exp)   (4 Exp.)   T cell proliferation                      GM-CSF   1.9   2.5   13.3           CpG   3.9   3.5   19.7           CpG + GM-CSF   7.0   8.5   25.6                    
U.S. Pat. No. 6,239,116 B1
 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Olionucleotide Stimulation of Mouse B Cells 
               
            
           
           
               
               
            
               
                   
                 Stimulation Index 1   
               
            
           
           
               
               
               
               
            
               
                 ODN 
                 Sequence (5′ to 3′)† 
                   3 H Uridine 
                 IgM Production 
               
               
                   
               
               
                 1 (SEQ ID NO: 1) 
                 GCTAGA CG TTAG CGT   
                 6.1 ± 0.8 
                 17.9 ± 3.6  
               
               
                   
               
               
                 1a (SEQ. ID NO: 42) 
                 ......T..... .. . 
                 1.2 ± 0.2 
                 1.7 ± 0.5 
               
               
                   
               
               
                 1b (SEQ ID NO: 3) 
                 ......Z..... .. . 
                 1.2 ± 0.1 
                 1.8 ± 0.0 
               
               
                   
               
               
                 1c (SEQ ID NO: 4) 
                 ...... .. ....Z.. 
                 10.3 ± 4.4  
                 9.5 ± 1.8 
               
               
                   
               
               
                 1d (SEQ ID NO: 5) 
                 ..AT.. .. ..GAGC. 
                 13.0 ± 2.3  
                 18.3 ± 7.5  
               
               
                   
               
               
                 2 (SEQ ID NO: 6) 
                 ATGGAAGGTCCAG CG TTCTC 
                 2.9 ± 0.2 
                 13.6 ± 2.0  
               
               
                   
               
               
                 2a (SEQ ID NO: 7) 
                 .. C. .CTC. .G .. .. ..... 
                 7.7 ± 0.8 
                 24.2 ± 3.2  
               
               
                   
               
               
                 2b (SEQ ID NO: 8) 
                 ..Z..CTC.ZG..Z...... 
                 1.6 ± 0.5 
                 2.8 ± 2.2 
               
               
                   
               
               
                 2c (SEQ ID NO: 9) 
                 ..Z..CTC. .G .. .. ..... 
                 3.1 ± 0.6 
                 7.3 ± 1.4 
               
               
                   
               
               
                 2d (SEQ ID NO: 10) 
                 .. C. .CTC. .G .. .. ..Z.. 
                 7.4 ± 1.4 
                 27.7 ± 5.4  
               
               
                   
               
               
                 2e (SEQ ID NO: 740) 
                 ............A .. ..... 
                 5.6 ± 2.0 
                 ND 
               
               
                   
               
               
                 3D (SEQ ID NO: 12) 
                 GAGAA CG CTGGACCTTCCAT 
                 4.9 ± 0.5 
                 19.9 ± 3.6  
               
               
                   
               
               
                 3Da (SEQ ID NO: 749) 
                 ..... .. .. .C ......... 
                 6.6 ± 1.5 
                 33.9 ± 6.8  
               
               
                   
               
               
                 3Db (SEQ ID NO: 14) 
                 ..... .. .. C. ..... .G .. 
                 10.1 ± 2.8  
                 25.4 ± 0.8  
               
               
                   
               
               
                 3Dc (SEQ ID NO: 15) 
                 ...C.A.............. 
                 1.0 ± 0.1 
                 1.2 ± 0.5 
               
               
                   
               
               
                 3Dd (SEQ ID NO: 17) 
                 .....Z.............. 
                 1.2 ± 0.2 
                 1.0 ± 0.4 
               
               
                   
               
               
                 3De (SEQ ID NO: 12) 
                 ..... .. ......Z...... 
                 4.4 ± 1.2 
                 18.8 ± 4.4  
               
               
                   
               
               
                 3Df (SEQ ID NO: 18) 
                 ..... .. A............ 
                 1.6 ± 0.1 
                 7.7 ± 0.4 
               
               
                   
               
               
                 3Dg (SEQ ID NO: 19) 
                 ..... .. ..CC.G.ACTG.. 
                 6.1 ± 1.5 
                 18.6 ± 1.5  
               
               
                   
               
               
                 3M (SEQ ID NO: 22) 
                 TCCATGT CG GTCCTGATGCT 
                 4.1 ± 0.2 
                 23.2 ± 4.9  
               
               
                   
               
               
                 3Ma (SEQ ID NO: 21) 
                 ......CT............ 
                 0.9 ± 0.1 
                 1.8 ± 0.5 
               
               
                   
               
               
                 3Mb (SEQ ID NO: 22) 
                 .......Z............ 
                 1.3 ± 0.3 
                 1.5 ± 0.6 
               
               
                   
               
               
                 3Mc (SEQ ID NO: 23) 
                 ....... .. ..Z........ 
                 5.4 ± 1.5 
                 8.5 ± 2.6 
               
               
                   
               
               
                 3Md (SEQ ID NO: 24) 
                 ......A .. T.......... 
                 17.2 ± 9.4  
                 ND 
               
               
                   
               
               
                 3Me (SEQ ID NO: 741) 
                 ...... .. .......C..A. 
                 3.6 ± 0.2 
                 14.2 ± 5.2  
               
               
                   
               
               
                 4 (SEQ ID NO: 26) 
                 TCAA CG TT 
                 6.1 ± 1.4 
                 19.2 ± 5.2  
               
               
                   
               
               
                 4a (SEQ ID NO: 27) 
                 ....GC.. 
                 1.1 ± 0.2 
                 1.5 ± 1.1 
               
               
                   
               
               
                 4b (SEQ ID NO: 28) 
                 ...G CG C. 
                 4.5 ± 0.2 
                 9.6 ± 3.4 
               
               
                   
               
               
                 4c (SEQ ID NO: 2306) 
                 ...T CG A. 
                 2.7 ± 1.0 
                 ND 
               
               
                   
               
               
                 4d (SEQ ID NO: 30) 
                 ..TT .. AA 
                 1.3 ± 0.2 
                 ND 
               
               
                   
               
               
                 4e (Residue 2-8 of 
                 -... .. .. 
                 1.3 ± 0.2 
                 1.1 ± 0.5 
               
               
                 SEQ ID NO: 26) 
                   
                   
                   
               
               
                 (SEQ ID NO: 31) 
                   
                   
                   
               
               
                   
               
               
                 4f (SEQ ID NO: 32) 
                 C... .. .. 
                 3.9 ± 1.4 
                 ND 
               
               
                   
               
               
                 4g (Residue 11-18 of 
                 --.. .. ..CT 
                 1.4 ± 0.3 
                 ND 
               
               
                 SEQ ID NO: 740) 
                   
                   
                   
               
               
                 (SEQ ID NO: 2307) 
                   
                   
                   
               
               
                   
               
               
                 4h (SEQ ID NO: 34) 
                 .... .. .C 
                 1.2 ± 0.2 
                 ND 
               
               
                   
               
               
                 LPS 
                   
                 7.8 ± 2.5 
                 4.8 ± 1.0 
               
               
                   
               
               
                   1 Stimulation indexes are the means and std. dev. derived from at least 3 separate experiments, and are compared to wells cultured with no added ODN. 
               
               
                 ND = not done. 
               
               
                 CpG dinuclectides are underlined. 
               
               
                 Dots indicate identity; 
               
               
                 dashes indicate deletions. 
               
               
                 Z indicates S methyl cytosine. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Identification of the optimal CpG motif for Murine IL-6 production and 
               
               
                 B cell activation 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                 IL-6 
                 (pg/ml) a   
                   
                   
               
               
                 ODN 
                 SEQUENCE (5′-3′) 
                   
                 CH12.LX 
                 SPLENIC B CELL 
                 SI b   
                 IgM (ng/ml) c   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 512 
                 (SEQ ID NO: 22) 
                 TCCATGT CG TCCTGATGCT 
                 1300 ± 106 
                 627 ± 43 
                 5.8 ± 0.3 
                  7315 ± 1324 
               
               
                   
               
               
                 1637 
                 (SEQ ID NO: 43) 
                 ......C .. ........... 
                 136 ± 27 
                 46 ± 6 
                 1.7 ± 0.2 
                 770 ± 72 
               
               
                   
               
               
                 1615 
                 (SEQ ID NO: 44) 
                 ......G .. ........... 
                 1201 ± 155 
                  850 ± 202 
                 3.7 ± 0.3 
                 3212 ± 617 
               
               
                   
               
               
                 1614 
                 (SEQ ID NO: 45) 
                 ......A .. ........... 
                 1533 ± 321 
                 1812 ± 103 
                 10.8 ± 0.6  
                 7558 ± 414 
               
               
                   
               
               
                 1636 
                 (SEQ ID NO: 46) 
                 ....... .. A.......... 
                 1181 ± 76  
                  947 ± 132 
                 5.4 ± 0.4 
                 3983 ± 485 
               
               
                   
               
               
                 1634 
                 (SEQ ID NO: 47) 
                 ....... .. C.......... 
                 1049 ± 223 
                 1671 ± 175 
                 9.2 ± 0.9 
                 6256 ± 261 
               
               
                   
               
               
                 1619 
                 (SEQ ID NO: 48) 
                 ....... .. T.......... 
                 1555 ± 304 
                 2908 ± 129 
                 12.5 ± 1.0  
                 8243 ± 698 
               
               
                   
               
               
                 1618 
                 (SEQ ID NO: 24) 
                 ......A .. T.......... 
                 2109 ± 291 
                 2596 ± 166 
                 12.9 ± 0.7  
                 10425 ± 674  
               
               
                   
               
               
                 1639 
                 (SEQ ID NO: 49) 
                 .....AA .. .T.......... 
                 1827 ± 83  
                 2012 ± 132 
                 11.5 ± 0.4  
                 9489 ± 103 
               
               
                   
               
               
                 1707 
                 (SEQ ID NO: 50) 
                 ......A .. TC......... 
                 ND 
                 1147 ± 175 
                 4.0 ± 0.2 
                 3534 ± 217 
               
               
                   
               
               
                 1708 
                 (SEQ ID NO: 51) 
                 .....CA .. TG......... 
                 ND 
                 59 ± 3 
                 1.5 ± 0.1 
                  466 ± 109 
               
               
                   
               
               
                 Dots indicate identity; CpG dinucleotides are underlined; ND = not done 
               
               
                   a The experiment was done at least three times with similar results. The level of IL-6 of unstimulated control cultures of both CH12.LX and splenic B cells was ≤10 pg/ml. The IgM level of unstimulated culture was 547 ± 82 ng/ml. CpG dinucleotides are underlined and dots indicate identity. 
               
               
                   b [ 3 H] Uridine uptake was indicated as a fold increase (SI: stimulation index) from unstimulated control (2322.67 ± 213.68 cpm). Cells were stimulated with 20 μM of various CpG O-ODN. Data present the mean ± SD of triplicates 
               
               
                   c Measured by ELISA. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
               
                   
               
               
                 Induction of Murine IL-6 secretion by CpG 
               
               
                 motifs in bacterial DNA or oligonucleotides. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Treatment 
                 IL-6 (pg/ml) 
               
               
                   
               
               
                   
                 calf thymus DNA 
                 ≤10 
               
               
                   
               
               
                   
                 calf thymus DNA + DNase 
                 ≤10 
               
               
                   
               
               
                   
                   E. coli  DNA 
                 1169.5 ± 94.1 
               
               
                   
               
               
                   
                   E. coli  DNA + DNase 
                 ≤10 
               
               
                   
               
               
                   
                 CpG methylated  E. coli  DNA 
                 ≤10 
               
               
                   
               
               
                   
                 LPS 
                  280.1 ± 17.1 
               
               
                   
               
               
                   
                 Media (no DNA) 
                 ≤10 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 ODN 
                   
                   
                   
               
               
                   
               
               
                 5a 
                 SEQ. ID. 
                 ATGGACTCTCCAG CG TTCTC 
                 1096.4 ± 372.0 
               
               
                   
                 No: 35 
                   
                   
               
               
                   
               
               
                 5b 
                 SEQ. ID. 
                 .....AGG....A .. ..... 
                 1124.5 ± 126.2 
               
               
                   
                 No: 740 
                   
                   
               
               
                   
               
               
                 5c 
                 SEQ. ID. 
                 .. C. ..... .G .. .. ..... 
                 1783.0 ± 189.5 
               
               
                   
                 No: 7 
                   
                   
               
               
                   
               
               
                 5d 
                 SEQ. ID. 
                 .... AGG..C..T...... 
                 ≤10 
               
               
                   
                 No: 742 
                   
                   
               
               
                   
               
               
                 5e 
                 SEQ. ID. 
                 .. C. ...... G. ..Z...... 
                  851.1 ± 114.4 
               
               
                   
                 No: 2308 
                   
                   
               
               
                   
               
               
                 5f 
                 SEQ. ID. 
                 ..Z......ZG..Z...... 
                 ≤10 
               
               
                   
                 No: 9 
                   
                   
               
               
                   
               
               
                 5g 
                 SEQ. ID. 
                 .. C. ..... .G .. .. ..Z.. 
                 1862.3 ± 87.26 
               
               
                   
                 No: 10 
               
               
                   
               
               
                 T cell depleted spleen cells from DBA/2 mice were stimulated with phosphodiester modified oligonucleotides (O-ODN) (20 μM), calf thymus DNA (50 μg/ml)or  E. Coli  DNA (50 μg/ml) with ot without enzyme treatment, or LPS (10 μg/ml) for 24 hr. 
               
               
                 Data represent the mean (pg/ml) ± SD of triplicates. 
               
               
                 CpG dinucleotides are underlined and dots indicate identity. 
               
               
                 Z indicates 5-methylcytosine. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Secretion of Murine IL-6 induced by CpG DNA 
               
               
                 stimulation in vivo. 
               
            
           
           
               
               
               
            
               
                   
                 Stimulant 
                 IL-6 (pg/ml) 
               
               
                   
               
               
                   
                 PBS 
                 &lt;50 
               
               
                   
               
               
                   
                   E. coli  DNA 
                 13858 ± 3143 
               
               
                   
               
               
                   
                 Calf Thymus DNA 
                 &lt;50 
               
               
                   
               
               
                   
                 CpG S-ODN 
                 20715 ± 606  
               
               
                   
               
               
                   
                 non-CpG S-ODN 
                 &lt;50 
               
               
                   
               
               
                 Mice (2 mice/group) were i.v. injected with 100 μl of PBS, 200 μg of  E. coli  DNA or calf. thymus DNA, or 500 μg of CpG S-ODN or non-CpG control S-ODN. Mice were bled 2 hr after injection and 1:10 dilution of each serum was analyzed by IL-6 ELISA. Sensitivity limit of IL-6 ELISA was 5 pg/ml. Sequences of the CpG S-ODN is 5′GCATGACGT-TGAGCT3′ (SEQ. ID. No: 6) and of the non-stimulatory S-ODN is 5′GCTAGATGTTAGCGT3′ (SEQ. ID. No: 49). Note that although there is a CpG in sequence 48, it is too close to the 3′ end to effect stimulation, as explained herein. Data represent mean ± SD of duplicates. The experiment was done at least twice with similar results. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Induction of human PBMC cytokine secrtetion by CpG oligos 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 ODN 
                 Sequence (5′-3′) 
                 IL-6 1   
                 TNF- α1   
                 IFN- γ1   
                 GM-CSF 
                 IL-12 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                  512 
                 TCCATGT CG GTCCTGATGCT 
                 500 
                 140 
                 15.6 
                 70 
                 250 
               
               
                 SEQ ID NO: 22 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1637 
                 ......C .. ........... 
                 550 
                 16 
                 7.8 
                 15.6 
                 16 
               
               
                 SEQ ID NO: 43 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1615 
                 ......G .. ........... 
                 600 
                 145 
                 7.8 
                 45 
                 145 
               
               
                 SEQ ID NO: 44 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1614 
                 ......A .. ........... 
                 550 
                 31 
                 0 
                 50 
                 31 
               
               
                 SEQ ID NO: 45 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1636 
                 ....... .. A.......... 
                 325 
                 250 
                 35 
                 40 
                 250 
               
               
                 SEQ ID NO: 46 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1634 
                 ....... .. C.......... 
                 300 
                 400 
                 40 
                 85 
                 400 
               
               
                 SEQ ID NO: 47 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1619 
                 ....... .. T.......... 
                 275 
                 450 
                 200 
                 80 
                 450 
               
               
                 SEQ ID NO: 48 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1618 
                 ......A .. T.......... 
                 300 
                 60 
                 15.6 
                 15.6 
                 62 
               
               
                 SEQ ID NO: 24 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1639 
                 .....AA .. T.......... 
                 625 
                 220 
                 15.6 
                 40 
                 220 
               
               
                 SEQ ID NO: 49 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1707 
                 ......A .. TC......... 
                 300 
                 70 
                 17 
                 0 
                 70 
               
               
                 SEQ ID NO: 50 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 1708 
                 .....CA .. TG......... 
                 270 
                 10 
                 17 
                 ND 
                 10 
               
               
                 SEQ ID NO: 51 
               
               
                   
               
               
                 dots indicate identity; 
               
               
                 CpG dinucleotides are underlined, measured by ELISA using Quantikine kits from R&amp;D Systems (pg/ml) Cells were caltured in 10% autologous serum with the indicated oligodeoxynucleotides (12 μg/ml) for 4 hr in the case of TNF-α or 24 hr for the other cytokines before supernatant harvest and assay. 
               
               
                 Data are presented as the level of cytokine above that in wells with no added oligodeoxynucleotide. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 CpG DNA induces cytokine secretion by human PBMC 
               
            
           
           
               
               
               
               
               
            
               
                   
                 TNF- 
                 IL-6 
                 IFN-γ 
                 RANTES 
               
               
                 DNA 
                 α (pg/ml) 1   
                 (pg/ml) 
                 (pg/ml) 
                 (pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 EC DNA (50 μg/ml) 
                 900 
                 12,000 
                 700 
                 1560 
               
               
                 EC DNA (5 μg/ml) 
                 850 
                 11,000 
                 400 
                 750 
               
               
                 EC DNA (0.5 μ/ml) 
                 500 
                 ND 
                 200 
                 0 
               
               
                 EC DNA (0.05 μg/ml) 
                 62.5 
                 10,000 
                 15.6 
                 0 
               
               
                 EC DNA (50 μg/ml) + 
                 0 
                 ND 
                 ND 
                 ND 
               
               
                 L-LME 2   
                   
                   
                   
                   
               
               
                 EC DNA (10 μg/ml) Methyl. 3   
                 0 
                 5 
                 ND 
                 ND 
               
               
                 CT DNA (50 μg/ml) 
                 0 
                 600 
                 0 
                 0 
               
               
                   
               
               
                   1 Levels of all cytokines were determined by ELISA using Quantikine kits from R&amp;D Systems as described in the previous table. Results are representative using PBMC from different donors. 
               
               
                   2 Cells were pretreated for 15 min. with L-leucyl-L-leucine methyl ester (M-LME) to determine whether the cytokine production under these conditions was from monocytes (or other L-LME-sensitive cells). 
               
               
                   3 EC DNA was methylated using 2 U/μg DNA of CpG methylase (New England Biolabs) according to the manufacturer&#39;s directions, and methylation confirmed by digestion with Hpa-II and Msp-I. As a negative control, samples were included containing twice the maximal amount of LPS contained in the highest concentration of EC DNA which failed to induce detectable cytokine production under these experimental conditions. 
               
               
                 ND = not done 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 CpG DNA induces cytokine expression in purified human macrophages 
               
            
           
           
               
               
               
               
            
               
                   
                 IL-6 (pg/ml) 
                 GM-CSF (pg/ml) 
                 TNF-α(pg/ml) 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 Cells alone 
                 0 
                 0 
                 0 
               
               
                 CT DNA (50 μg/ml) 
                 0 
                 0 
                 0 
               
               
                 EC DNA (50 μg/ml) 
                 2000 
                 15.6 
                 1000 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Induction Of NK Activity By CpG Oligodeoxynucleotides (ODN) 
               
            
           
           
               
               
               
            
               
                   
                 % YAC-1 Specific Lysis* 
                 % 2C11 Specific Lysis 
               
               
                   
                 Effector:Target 
                 Effector:Target 
               
            
           
           
               
               
               
               
               
            
               
                 ODN 
                 50:1 
                 100:1 
                 50:1 
                 100:1 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 None 
                 −1.1 
                 −1.4 
                 15.3 
                 16.6 
               
               
                 1 
                 16.1 
                 24.5 
                 38.7 
                 47.2 
               
               
                 3Dd 
                 17.1 
                 27.0 
                 37.0 
                 40.0 
               
               
                 non-CpG ODN 
                 −1.6 
                 −1.7 
                 14.8 
                 15.4 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Induction of NK Activity by DNA Containing CpG Motifs but not by Non- 
               
               
                 CpG DNA 
               
            
           
           
               
               
            
               
                   
                 LU/10 6   
               
            
           
           
               
               
               
               
            
               
                   
                 DNA or Cytokine Added 
                 Mouse Cells 
                 Human Cells 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 Expt. 1 
                 None 
                 0.00 
                 0.00 
               
               
                   
                 IL-2 
                 16.68 
                 15.82 
               
               
                   
                   E. Coli , DNA 
                 7.23 
                 5.05 
               
               
                   
                 Calf thymus DNA 
                 0.00 
                 0.00 
               
               
                   
               
               
                 Expt. 2 
                 None 
                 0.00 
                 3.28 
               
               
                   
                 1585 ggGGTCAA CG TTGACgggg (SEQ ID No. 52) 
                 7.38 
                 17.98 
               
               
                   
                 1629 -------gtc----- (SEQ ID No. 750) 
                 0.00 
                 4.4 
               
               
                   
               
               
                 Expt. 3 
                 None 
                 0.00 
                   
               
               
                   
                 1613 GCTAGA CG TTAGTGT (SEQ ID No. 54) 
                 5.22 
                   
               
               
                   
                 1769 -------Z----- (SEQ ID No. 745) 
                 0.02 
                 ND 
               
               
                   
                 1619 TCCATGT CG TTCCTGATGCT (SEQ ID No: 48) 
                 3.35 
                   
               
               
                   
                 1765 -------Z--------- (SEQ ID No. 56) 
                 0.11 
               
               
                   
               
               
                 CpG dinuoleotides in ODN sequences are indicated by underlying; 
               
               
                 Z indicates methylcytosine. 
               
               
                 Lower case letters indicate nuclease resistant phosphorothioate modified internucleotide linkages which, in titration experiments, were more than 20 times as potent as non-modified ODN, depending on the flanking bases. 
               
               
                 Poly G ends (g) were used in some ODN, because they significantly increase the level of ODN uptake. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 ODN induction of NK Lytic Activity (LU) 
               
            
           
           
               
               
               
               
            
               
                 ODN 
                   
                   
                   
               
               
                 cells 
                   
                 LU 
                   
               
               
                 alone 
                 Sequence (5′-3′) 
                 0.01 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 1754 
                 ACCATGGACGATCTGTTTCCCCTC 
                 0.02 
                 SEQ ID NO: 58 
               
               
                   
               
               
                 1758 
                 TCTCCCAGCGTGCGCCAT 
                 0.05 
                 SEQ ID NO: 59 
               
               
                   
               
               
                 1761 
                 TACCGCGTGCGACCCTCT 
                 0.05 
                 SEQ ID NO: 60 
               
               
                   
               
               
                 1776 
                 ACCATGGACGAACTGTTTCCCCTC 
                 0.03 
                 SEQ ID NO: 61 
               
               
                   
               
               
                 1777 
                 ACCATGGACGAGCTGTTTCCCCTC 
                 0.05 
                 SEQ ID NO: 62 
               
               
                   
               
               
                 1778 
                 ACCATGGACGTACTGTTTCCCCTC 
                 0.01 
                 SEQ ID NO: 63 
               
               
                   
               
               
                 1779 
                 ACCATGGACGTACTGTTTCCCCTC 
                 0.02 
                 SEQ ID NO: 64 
               
               
                   
               
               
                 1780 
                 ACCATGGACGGTCTGTTTCCCCTC 
                 0.29 
                 SEQ ID NO: 65 
               
               
                   
               
               
                 1781 
                 ACCATGGACGTTCTGTTTCCCCTC 
                 0.38 
                 SEQ ID NO: 66 
               
               
                   
               
               
                 1823 
                 GCATGACGTTGAGCT 
                 0.08 
                 SEQ ID NO: 41 
               
               
                   
               
               
                 1824 
                 CACGTTGAGGGGCAT 
                 0.01 
                 SEQ ID NO: 67 
               
               
                   
               
               
                 1825 
                 CTGCTGAGACTGGAG 
                 0.01 
                 SEQ ID NO: 68 
               
               
                   
               
               
                 1828 
                 TCAGCGTGCGCC 
                 0.01 
                 SEQ ID NO: 69 
               
               
                   
               
               
                 1829 
                 ATGACGTTCCTGACGTT 
                 0.42 
                 SEQ ID NO: 70 
               
               
                   
               
               
                 1830 2   
                 RANDOM SEQUENCE 
                 0.25 
                   
               
               
                   
               
               
                 1834 
                 TCTCCCAGCGGGCGCAT 
                 0.00 
                 SEQ ID NO: 71 
               
               
                   
               
               
                 1836 
                 TCTCCCAGCGCGCGCCAT 
                 0.46 
                 SEQ ID NO: 72 
               
               
                   
               
               
                 1840 
                 TCCATGTCGTTCCTGTCGTT 
                 2.70 
                 SEQ ID NO: 73 
               
               
                   
               
               
                 1841 
                 TCCATAGCGTTCCTAGCGTT 
                 1.45 
                 SEQ ID NO: 74 
               
               
                   
               
               
                 1842 
                 TCGTCGCTGTCTCCGCTTCTT 
                 0.06 
                 SEQ ID NO: 75 
               
               
                   
               
               
                 1851 
                 TCCTGACGTTCCTGACGTT 
                 2.32 
                 SEQ ID NO: 76 
               
               
                   
               
               
                   1 Lytic unite (LU) were measured as described (8). Briefly, PBMC were collected from normal donors and spun over Ficoll, then cultured with or without the indicated ODN (which were added to cultures at 6 μg/ml) for 24 hr. Then their ability to lyse  51 Cr-labeled K562 cells was determined. The results shown are typical of those obtained with several different normal human donors. 
               
               
                   2 This oligo mixture contained a random selection of all 4 bases at each position. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Induction of NK LU by Phoshorothioate CpG ODN with Good Motifs 
               
            
           
           
               
               
               
               
               
               
            
               
                 ODN 1   
                   
                   
                   
                   
                   
               
               
                 cells 
                   
                   
                 expt. 1 
                 expt. 2 
                 expt. 3 
               
               
                 alone 
                 sequence (5′-3′) 
                 SEQ ID NO: 
                 0.00 
                 1.26 
                 0.46 
               
               
                   
               
               
                 1840 
                 TCCATGT CG TTCCTGT CG TT 
                 73 
                 2.33 
                 ND 
                 ND 
               
               
                   
               
               
                 1960 
                 TCCTGT CG TTCCTGT CG TT 
                 77 
                 ND 
                 0.48 
                 8.99 
               
               
                   
               
               
                 1961 
                 TCCATGT CG TTTTTGT CG TT 
                 78 
                 4.03 
                 1.23 
                 5.08 
               
               
                   
               
               
                 1962 
                 TCCTGT CG TTCCTTGT CG TT 
                 79 
                 ND 
                 1.60 
                 5.74 
               
               
                   
               
               
                 1963 
                 TCCTTGT CG TTCCTGT CG TT 
                 80 
                 3.42 
                 ND 
                 ND 
               
               
                   
               
               
                 1965 
                 TCCTGT CG TTTTTTGT CG TT 
                 81 
                 0.46 
                 0.42 
                 3.48 
               
               
                   
               
               
                 1966 
                 T CG T CG CTGTCTC CG CTTCTT 
                 75 
                 2.62 
                 ND 
                 ND 
               
               
                   
               
               
                 1967 
                 T CG T CG CTGTCTGCCCTTCTT 
                 82 
                 5.82 
                 1.64 
                 8.32 
               
               
                   
               
               
                 1968 
                 T CG T CG CTGTTGT CG TTTCTT 
                 83 
                 3.77 
                 5.26 
                 5.12 
               
               
                   
               
               
                 1979 2   
                 TCCATGTZGTTCCTGTZGTT 
                 84 
                 1.32 
                 ND 
                 ND 
               
               
                   
               
               
                 1982 
                 TCCAGGACTTCTCTCAAGTT 
                 751 
                 0.05 
                 ND 
                 0.98 
               
               
                   
               
               
                 1990 
                 TCCATG CG TG CG TG CG TTTT 
                 86 
                 2.10 
                 ND 
                 ND 
               
               
                   
               
               
                 1991 
                 TCCATG CG TTG CG TTG CG TT 
                 87 
                 0.89 
                 ND 
                 ND 
               
               
                   
               
               
                 2002 
                 TCCA CG A CG TTTT CG A CG TT 
                 88 
                 4.02 
                 1.31 
                 9.79 
               
               
                   
               
               
                 2005 
                 T CG T CG TTGT CG TTGT CG TT 
                 89 
                 ND 
                 4.22 
                 12.73  
               
               
                   
               
               
                 2006 
                 T CG T CG TTTTGT CG TTTTGT CG T 
                 752 
                 ND 
                 6.17 
                 12.82  
               
               
                   
               
               
                 2007 
                 T CG T CG TTGT CG TTTTGT CG TT 
                 91 
                 ND 
                 2.68 
                 9.65 
               
               
                   
               
               
                 2008 
                 G CG TG CG TTGT CG TTGT CG TT 
                 92 
                 ND 
                 1.37 
                 8.15 
               
               
                   
               
               
                 2010 
                 G CG G CG GG CG G CGCGCG CCC 
                 93 
                 ND 
                 0.01 
                 0.05 
               
               
                   
               
               
                 2012 
                 TGT CG TTTGT CG TTTGT CG TT 
                 94 
                 ND 
                 2.02 
                 11.61  
               
               
                   
               
               
                 2013 
                 TGT CG TTGT CG TTGT CG TTGT CG TT 
                 95 
                 ND 
                 0.56 
                 5.22 
               
               
                   
               
               
                 2014 
                 TGT CG TTGT CG TTGT CG TT 
                 96 
                 ND 
                 5.74 
                 10.89  
               
               
                   
               
               
                 2015 
                 T CG T CG T CG T CG TT 
                 97 
                 ND 
                 4.53 
                 10.13  
               
               
                   
               
               
                 2016 
                 TGT CG TTGT CG TT 
                 98 
                 ND 
                 6.54 
                 8.06 
               
               
                   
               
               
                   1 PBMC essentially as described herein. Results are representative of 6 separate experiments; each experiment represents a different donor. 
               
               
                   2 This is the methylated version of ODN 1840 (SEQ ID NO: 83); Z = 5-methyl cytosine at residues 8 and 17; LU is lytic units; ND = not done; CpG dinucleotides are underlined for clarity 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 Induction of human B cell proliferation by Phosphorothicate CpG ODN 
               
            
           
           
               
               
            
               
                   
                 Stimulation Index 1L   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 ODN 
                 sequence (5′ 3′) 
                 SEQ ID NO: 
                 expt. 1 
                 expt. 2 
                 expt. 3 
                 expt. 4 
                 expt. 5 
                 expt. 6 
               
               
                   
               
               
                 1840 
                 TCCATGT CG TTCCTGT CG TT 
                 73 
                 4 
                 ND 
                 ND 
                 ND 
                 ND 
                 34 
               
               
                   
               
               
                 1841 
                 TCCATAG CG TTCCTAG CG TT 
                 74 
                 3 
                 ND 
                 ND 
                 ND 
                 ND 
                 ND 
               
               
                   
               
               
                 1960 
                 TCCTGT CG TTCCTGT CG TT 
                 77 
                 ND 
                 2.0 
                 2.0 
                 3.6 
                 ND 
                 ND 
               
               
                   
               
               
                 1961 
                 TCCATGT CG TTTTTGT CG TT 
                 78 
                 2 
                 3.9 
                 1.9 
                 3.7 
                 ND 
                 37 
               
               
                   
               
               
                 1962 
                 TCCTGT CG TTCCTTGT CG TT 
                 79 
                 ND 
                 3.9 
                 1.9 
                 3.9 
                 5.4 
                 35 
               
               
                   
               
               
                 1963 
                 TCCTTGT CG TTCCTGT CG TT 
                 80 
                 3 
                 ND 
                 ND 
                 ND 
                 ND 
                 ND 
               
               
                   
               
               
                 1965 
                 TCCTGT CG TTTTTTGT CG TT 
                 81 
                 4 
                 3.7 
                 2.4 
                 4.7 
                 6.0 
                 43 
               
               
                   
               
               
                 1967 
                 T CG T CG TGTCTGCCCTTCTT 
                 82 
                 ND 
                 4.4 
                 2.0 
                 4.5 
                 5.0 
                 38 
               
               
                   
               
               
                 1968 
                 T CG T CG CTGTGT CG TTTCTT 
                 83 
                 ND 
                 4.0 
                 2.0 
                 4.9 
                 8.7 
                 38 
               
               
                   
               
               
                 1982 
                 TCCAGGACTTCTCTCAGGTT 
                 85 
                 3 
                 1.8 
                 1.3 
                 3.1 
                 3.2 
                 12 
               
               
                   
               
               
                 2002 
                 TCCA CG A CG TTTT CG A CG TT 
                 88 
                 ND 
                 2.7 
                 1.4 
                 4.4 
                 ND 
                 14 
               
               
                   
               
               
                 2005 
                 T CG T CG TTGT CG TTGT CG TT 
                 89 
                 5 
                 3.2 
                 1.2 
                 3.0 
                 7.9 
                 37 
               
               
                   
               
               
                 2006 
                 T CG T CG TTTTGT CG TTTTGT CG TT 
                 90 
                 4 
                 4.5 
                 2.2 
                 5.8 
                 8.3 
                 40 
               
               
                   
               
               
                 2007 
                 T CG T CG TTGT CG TTTTGT CG TT 
                 91 
                 3 
                 4.0 
                 4.2 
                 4.1 
                 ND 
                 22 
               
               
                   
               
               
                 2008 
                 G CG TG CG TTGT CG TTGT CG TT 
                 92 
                 ND 
                 3.0 
                 2.4 
                 1.6 
                 ND 
                 12 
               
               
                   
               
               
                 2010 
                 G CG G CG GG CG G CGCGCG CCC 
                 93 
                 ND 
                 1.6 
                 1.9 
                 3.2 
                 ND 
                 ND 
               
               
                   
               
               
                 2012 
                 TGT CG TTTGT CG TTTGT CG TT 
                 94 
                 2 
                 2.8 
                 0 
                 3.2 
                 ND 
                 33 
               
               
                   
               
               
                 2013 
                 TGT CG TTGT CG TTGT CG TTGT CG TT 
                 95 
                 3 
                 2.3 
                 3.1 
                 2.8 
                 ND 
                  7 
               
               
                   
               
               
                 2014 
                 TGT CG TTGT CG TTGT CG TT 
                 96 
                 3 
                 2.5 
                 4.0 
                 3.2 
                 6.7 
                 14 
               
               
                   
               
               
                 2015 
                 T CG T CG T CG T CG TT 
                 97 
                 5 
                 1.8 
                 2.6 
                 4.5 
                 9.4 
                  1 
               
               
                   
               
               
                 2016 
                 TGT CG TTGT CG TT 
                 98 
                 ND 
                 1.1 
                 1.7 
                 2.7 
                 7.3 
                  1 
               
               
                   
               
               
                   1 Cells = human spleen cells stored at −70° C. after surgical harvest or PBNC collected from normal donors and spun over Ficoll. Cells were cultured in 96 well U-bottom microtiter lates with or without the indicated ODN (which were added to cultures at 6 μml). N = 12 experiments. Cells were cultured for 4-7 days, pulsed with 1 μCi of  3 H thymidine for 18 hr before harvest and scintillation counting. Stimulation index = the ratio of cpm in wells without ODN to that in wells that had been stimulated throughout the culture period with the indicated ODN (there were no further additions of ODN after the culture were set up). 
               
               
                 ND = not done 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 Induction of human IL-12 secretion by 
               
               
                 Phophorothicate CpG ODN 
               
            
           
           
               
               
               
            
               
                   
                 SEQ 
                 IL-12 (pg/ml) 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                 ID 
                 expt. 
                 expt. 
               
               
                 ODN 1   
                 sequence (5′-3′) 
                 no 
                 1 
                 2 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 cells 
                   
                   
                 0 
                 0 
               
               
                 alone 
                   
                   
                   
                   
               
               
                   
               
               
                 1962 
                 TCCTGTCGTTCCTTGTCGTT 
                 79 
                 19 
                 0 
               
               
                   
               
               
                 1965 
                 TCCTGTCGTTTTTTGTCGTT 
                 81 
                 36 
                 0 
               
               
                   
               
               
                 1967 
                 TCGTCGCTGTCTGCCCTTCTT 
                 82 
                 41 
                 0 
               
               
                   
               
               
                 1968 
                 TCGTCGCTGTTGTCGTTTCTT 
                 83 
                 24 
                 0 
               
               
                   
               
               
                 2005 
                 TCGTCGTTGTCGTTGTCGTT 
                 89 
                 25 
                 0 
               
               
                   
               
               
                 2006 
                 TCGTCGTTTTGTCGTTTTGTCGTT 
                 90 
                 29 
                 15 
               
               
                   
               
               
                 2014 
                 TGTCGTTGTCGTTGTCGTT 
                 96 
                 28 
                 0 
               
               
                   
               
               
                 2015 
                 TCGTCGTCGTCGTT 
                 97 
                 14 
                 0 
               
               
                   
               
               
                 2016 
                 TGTCGTTGTCGTT 
                 98 
                 3 
                 0 
               
               
                   
               
               
                   1 PBMC were collected from normal donors and spun over Ficoll, then cultured at 10 6  cells/well in 96 well microtiter plates with or without the indicated ODN which were added to cultures at 6 μg/ml. Supernatants were collected at 24 hr and tested for IL-12 levels by ELISA as described in methods. A standard curve was run in each experiment, which represents a different donor. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Different CpG motifs stimulate optimal 
               
               
                 murine B cell and NK activation 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 B cell 
                 NK 
               
               
                 ODN 
                 Sequence 
                 activation 1   
                 activation 2   
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 1668 
                 TCCATGA CG TTCCTGATGCT 
                 42,849 
                 2.52 
               
               
                   
                 (SEQ. ID. NO: 24) 
                   
                   
               
               
                   
               
               
                 1758 
                 TCTCCCAG CG TG CG CCAT 
                 1,747 
                 6.66 
               
               
                   
                 (SEQ. ID. NO: 59) 
                   
                   
               
               
                   
               
               
                 NONE 
                   
                 367 
                 0.00 
               
               
                   
               
               
                 CpG dinucleotides are underlined; oligonucleotides were synthesized with phosphorothioate modified backbones to improve their nuclease resistance. 
               
               
                   1 Measured by  3 H thymidine incorporation after 48 hr culture with oligodeoxynucleotides at a 200 nM concentration as described in Example 1. 
               
               
                   2 Measured in lytic units. 
               
            
           
         
       
     
                         TABLE 15                      Specific blockade of CpG-induced TNF-α and IL-12 expression by inhibitors           of endosomal acidification or NFκB activation                                                         Inhibitors:           Glio-   Bisglio-                                                                 Bafilomycin   Chloroquine   Monensin   NAC   TPCK   toxin   toxin                                                     Medium   (250 nM)   (2.5 μg/ml)   (10 μM)   (50 mM)   (50 μM)   (0.1 μg/ml)   (0.1 μg/ml)                                                                 activators   TNF-α   IL-12   TNF-α   IL-12   TNF-α   IL-12   TNF-α   IL-12   TNF-α   TNF-α   TNF-α   TNF-α                                                                         Medium   37   147   46   102   27   20   22   73   10   24   17   41       CpG   455   17,114   71   116   28   6   49   777   54   23   31   441       ODN                                                       LPS   901   22,485   1370   4051   1025   12418   491   4796   417   46   178   1120               TABLE 15 legend IL-12 and TNF-α assays: The murine monocyte cell line 3774 (1 × 10 5  cells/ml for IL 12 or 1 × 10 6  cells/ml for TNF-α), were cultured with or without the indicated inhibitors at the concentrations shown for 2 hr and then stimulated with the CpG oligodeoxynucleotide (ODN) 1826 (TCCATGACGTTCCTGACGTT SEQ ID NO: 100) at 2 μm or LPS (10 μg/ml) for 4 hr (TNF-α or 24 hr (IL-12) at which time the supernatant was harvested. ELISA for IL-12 or TNF-α (pg/ml) was performed on the supernatants essentially as described (A.K. Krieg, A.-K. Yi, S. Matson, T.J. Waldschmidt, G.A. Bishop, R. Teasdale, G. Koretzy and D. Klinman, Nature 374, 546 (1995); Yi A.-K., D.M. Klinman, T.L. Martin, S. Matson and A.M. Krieg. J Immunol., 157, 5394-5402 (1996); Krieg, A.M, J Lab. Clin. Med., 128, 128-133 (1996). Cells cultured with ODN that lacked CpG motifs did not induce cytokine secretion. Similar specific inhibition of CpG responses was seen with IL-6 assays, and in experiments using primary spleen cells or the B cell lines CH12.LX and WEHI-231.2.5 μg/ml of chloroquine is equivalent to &lt;5 μM. Other inhibitors of NF-κB activation including PDTC and calpain inhibitors I and II gave similar results to the inhibitors shown. The results shown are representative of those obtained in ten different experiments.            
U.S. Pat. No. 6,339,068 B1
 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Plasmids containing immunostimulatory CpG motifs 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 Species specificity and 
               
               
                   
                   
                 No. CpG, 
                 ODN Equivalence of CpG-S 
               
               
                 Plasmid 
                 Backbone 
                 Motifs 
                 Insert 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 pMCG-16 
                 pMAS 
                 16 
                 mouse-specific CpG motif 
               
               
                 pMCG-50 
                 pMAS 
                 50 
                 #1826 1   
               
               
                 pMCG- 
                 pMAS 
                 300 
                   
               
               
                 100 
                   
                   
                   
               
               
                 pMCG- 
                 pMAS 
                 200 
                   
               
               
                 200 
                   
                   
                   
               
               
                   
               
               
                 pHCG-30 
                 pMAS 
                 30 
                 human-specific CpG motif- 
               
               
                 pHCG-50 
                 pMAS 
                 50 
                 no ODN equivalent 2   
               
               
                 pHCG- 
                 pMAS 
                 100 
                   
               
               
                 100 
                   
                   
                   
               
               
                 pHCG- 
                 pMAS 
                 200 
                   
               
               
                 200 
                   
                   
                   
               
               
                   
               
               
                 pHIS-40 
                 pMAS 
                 40 
                 human-specific CpG motif 
               
               
                 pHIS-64 
                 pMAS 
                 64 
                 #2006 3   
               
               
                 pHIS- 
                 pMAS 
                 128 
                   
               
               
                 128 
                   
                   
                   
               
               
                 pHIS- 
                 pMAS 
                 192 
                   
               
               
                 192 
               
               
                   
               
               
                   1 sequence of 1826 is TCCATGA C GTTCCTGA CG TT (SEQ ID NO. 100) 
               
               
                   2 sequence used as a source of CpG motifs is GACTT  CG TGT CG TTCTTCTGT CG TCTTTAG CG CTTCTCCTGCGTG  CG TCCCTTG(SEQ ID NO: 753) 
               
               
                   3 sequence of 2006 is T CG T CG TTTTGT CG TTTTGT CG TT(SEQ ID NO: 90) 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Plasmids encoding hepatitis B surface antigen 
               
               
                 (derived from ayw or adw subtypes of HBV) 
               
            
           
           
               
               
               
               
            
               
                   
                 Plasmid 
                 Backbone 
                 Insert 
               
               
                   
               
               
                   
                 pUK-S 
                 pUK21-A2 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pUKAX-S 
                 pUK21-AX* 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pMAS-S 
                 pMAS 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pMCG16-S 
                 pMCG-16 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pMCG50-S 
                 pMCG-50 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pMCG100-S 
                 pMCG-100 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pMCG200-S 
                 pMCG-200 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pHCG30-S 
                 pHCG-30 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pHCG50-S 
                 pHCG-50 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pHCG100-S 
                 pHCG-100 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pHCG200-S 
                 pHCG-200 
                 HBV-S (ayw) 
               
               
                   
               
               
                   
                 pHIS40-S(ad) 
                 pHIS-40 
                 HBV-S(adw2) 
               
               
                   
               
               
                   
                 pHIS64-S(ad) 
                 pHIS-64 
                 HBV-S(adw2) 
               
               
                   
               
               
                   
                 pHIS128-S(ad) 
                 pHIS-128 
                 HBV-S(adw2) 
               
               
                   
               
               
                   
                 pHIS192-S(ad) 
                 pHIS-192 
                 HBV-S(adw2) 
               
               
                   
               
               
                 *pUK21-AX was created by deleting f1 origin from pUK21-A 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 ODN used with plasmid DNA 
               
            
           
           
               
               
               
            
               
                   
                 ODN 
                   
               
               
                 Backbone 
                 code number 
                 Sequence 
               
               
                   
               
               
                 S-ODN 
                 1826 
                 (SEQ ID NO: 100) 
               
               
                   
                   
                 TCCATGA CG TTCCTGA CG TT 
               
               
                   
                 1628 
                 (SEQ ID NO: 754) 
               
               
                   
                   
                 GGGGTCAA CG TTGAGGGGGG 
               
               
                   
                 1911 
                 (SEQ ID NO: 755) 
               
               
                   
                   
                 TCCAGGACTTTCCTCAGGTT 
               
               
                   
                 1982 
                 (SEQ ID NO: 85) 
               
               
                   
                   
                 TCCAGGACTTCTCTCAGGTT 
               
               
                   
                 2017 
                 (SEQ ID NO: 756) 
               
               
                   
                   
                 CCCCCCCCCCCCCCCCCCCC 
               
               
                   
               
               
                 O-ODN 
                 2061 
                 (SEQ ID NO: 100) 
               
               
                   
                   
                 TCCATGA CG TTCCTGA CG TT 
               
               
                   
                 2001 
                 (SEQ ID NO: 757) 
               
               
                   
                   
                 GG CG G CG G CG G CG G CG G CG G 
               
               
                   
               
               
                 SOS-ODN 
                 1980 
                 (SEQ ID NO: 100) 
               
               
                   
                   
                 TCCATGA CG TTCCTGA CG TT 
               
               
                   
                 1585 
                 (SEQ ID NO: 758) 
               
               
                   
                   
                 GGGTCAA CG TTGAGGGGGG 
               
               
                   
                 1844 
                 (SEQ ID NO: 759) 
               
               
                   
                   
                 TCTCCCAG CG TG CG CCATAT 
               
               
                   
                 1972 
                 (SEQ ID NO: 760) 
               
               
                   
                   
                 GGGGTCTGTGCTTTTGGGGGG 
               
               
                   
                 2042 
                 (SEQ ID NO: 761) 
               
               
                   
                   
                 TCAGGGGTGGGGGGAACCTT 
               
               
                   
                 1981 
                 (SEQ ID NO: 762) 
               
               
                   
                   
                 GGGGTTGA CG TTTTGGGGGG 
               
               
                   
                 2018 
                 (SEQ ID NO: 763) 
               
               
                   
                   
                 TCTAG CG TTTTTAG CG TTCC 
               
               
                   
                 2021 
                 (SEQ ID NO: 89) 
               
               
                   
                   
                 T CG T CG TTGT CG TTGT CG TT 
               
               
                   
                 2022 
                 (SEQ ID NO: 90) 
               
               
                   
                   
                 T CG T CG TTTTGT CG TTTTGT C GTT 
               
               
                   
                 2023 
                 (SEQ ID NO: 91) 
               
               
                   
                   
                 T CG T CG TTGT CG TTTTGT CG TT 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
             
               
                 TABLE 10 
               
               
                   
               
             
            
               
                   
                 Inhibitory CpG motifs can block B cell 
               
               
                   
                 proliferation induced by a stimulatory 
               
               
                   
                 CpG motif 
               
            
           
           
               
               
            
               
                 Oligonucleotide added 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 medium 
                   
                 194 
               
               
                 1968 
                 (TCCATGACGTTCCTGATGCT 
                 34,669 
               
               
                   
                 (SEQ ID NO: 24) 
                 24,452 
               
               
                   
               
               
                 1668 
                 +1735 (GCGTTTTTTTTTGCG) 
                 601 
               
               
                   
                 (SEQ ID NO: 764) 
                   
               
               
                   
               
               
                 1720 
                 (TCCATGAGCTTCCTGATGCT) 
                 1109 
               
               
                   
                 (SEQ ID NO: 765) 
                   
               
               
                   
               
               
                 1720 
                 +1735 
               
               
                   
               
            
           
           
               
               
            
               
                   
                 Inhibitory effects of “bad” CpG 
               
               
                   
                 motifs on the “good” CpG Oligo 1619 
               
            
           
           
               
               
            
               
                   
                 I: −12 in 
               
               
                 Oligonucleotide added 
                 pg/ml 
               
               
                   
               
            
           
           
               
               
               
            
               
                 medium 
                   
                 0 
               
               
                   
               
               
                 1619 
                 alone 
                 6 
               
               
                   
               
               
                 1619 
                 1949 (TCCATGTCGTTCCTGATGCG 
                 16 
               
               
                   
                 (SEQ ID NO: 766)) 
                   
               
               
                   
               
               
                 1619 
                 1952 (TCCATGTCGTTCCGCGCGCG 
                 0 
               
               
                   
                 (SEQ ID NO: 767)) 
                   
               
               
                   
               
               
                 1619 
                 1953 (TCCATGTCGTTCCTGCCGCT 
                 0 
               
               
                   
                 (SEQ ID NO: 768)) 
                   
               
               
                   
               
               
                 1619 
                 1953 (GCGGCGGGCGGCGCGCGCCC 
                 0 
               
               
                   
                 (SEQ ID NO: 93)) 
               
               
                   
               
               
                   1 Dots 1720in the sequence of ODN 1952 and 1953 indicate identity to ODN 1619; CpG dinucleotides are underlined for clarity. ODN without CpG-N or CpG-S motifs had little or no effect on cytokine production. The data shown are representative of 4 experiments. 
               
               
                   2 All cytokines are given in pg/ml; measured by ELISA on supernatants from DBA/2 spleen cells cultured in 96 well plates at 2 × 10 7  cells/ml for 24 hr with the indicated ODN at 30 μg/ml. Std. dev. of the triplicate wells was &lt;7%. None of the ODN induced significant amounts of IL-5. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 Identification of neutralizing CpG motifs 
               
               
                 which reduce the induction of cytokine 
               
               
                 secretion by a CpG-S motif in the same 
               
               
                 ODN (cis-neutralization) 
               
            
           
           
               
               
            
               
                   
                 ODN-induced cytokine 
               
               
                   
                 expression 2   
               
            
           
           
               
               
               
               
               
            
               
                 ODN 
                 sequence 5′-3′ 1   
                 IL-6 2   
                 IL-12 
                 IFN-γ 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 None 
                   
                 &lt;5 
                 206 
                 898 
               
               
                   
               
               
                 1619 
                 TCCATGT CG TTCCTGATGCT 
                 1405 
                 3130 
                 4628 
               
               
                   
                 (SEQ ID NO: 48) 
                   
                   
                   
               
               
                   
               
               
                 1952 
                 ....... .. ....G CGCGCG   
                 559 
                 1615 
                 2135 
               
               
                   
                 (SEQ ID NO: 767) 
                   
                   
                   
               
               
                   
               
               
                 1953 
                 ....... .. ......C C. .. 
                   
                   
                   
               
               
                   
                 (SEQ ID NO: 768) 
               
               
                   
               
               
                   1 Dots in the sequence of ODN 1952 and 1953 indicate identity to ODN 1619; CpG dinucleotides are underlined for clarity. ODN without CpG-N or CpG-S motifs had little or no effect on cytokine production. The data shown are representative of 4 experiments. 
               
               
                   2 All cytokines are given in pg/ml; measured by ELISA on supernatants from DBA/2 spleen cells cultured in 96 well plates at 2 × 10 7  cells/ml for 24 hr with the indicated ODN at 30 μg/ml. Std. dev. of the triplicate wells was &lt;7%. None of the ODN induced significant amounts of IL-5. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Inhibition of CpG-induced cytokine 
               
               
                 secretion by ODN containing CpG-N motifs 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 CpG-S- 
               
               
                   
                   
                   
                 induced 
               
               
                   
                   
                 IL-12 
                 IL-12 
               
               
                 ODN 
                 sequence 5′-3′ 
                 secretion 1   
                  secretion 2   
               
               
                   
               
               
                 none 
                   
                 268 
                 5453 
               
               
                   
               
               
                 1895 
                 G CGCGCGCGCGCGCGCGCG C 
                 123 
                 2719 
               
               
                   
                 (SEQ ID NO: 769) 
                   
                   
               
               
                   
               
               
                 1896 
                 C CG GC CG GC CG GC CG GC CG G 
                 292 
                 2740 
               
               
                   
                 (SEQ ID NO: 770) 
                   
                   
               
               
                   
               
               
                 1955 
                 G CG G CG GG CG G CGCGCG CCC 
                 270 
                 2539 
               
               
                   
                 (SEQ ID NO: 93) 
                   
                   
               
               
                   
               
               
                 2037 
                 TCCATGC CG TTCCTGC CG TT 
                 423 
                 2847 
               
               
                   
                 (SEQ ID NO: 771) 
               
               
                   
               
               
                   1 BALB/c spleen cells were cultured in 96 well plates at 2 × 10 7  cells/ml with the indicated ODN for 24 hr and then the supernatants were assayed for IL-12 by ELISA (pg/ml). 
               
               
                   2 Cells were set up the same as in  1  except that IL-12 secretion was induced by the addition of the CpG ODN 1619 (TCCATGTCGTTCCTGATGCT) (SEQ ID NO: 48) at 30 μg/ml. The data shown are representative of 5 experiments. 
               
            
           
         
       
     
     APPENDIX (C) 
     
       
         
           
               
             
               
                   
               
               
                 Exemplary Human miRNA Sequences 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 &gt;hsa-let-7a-1 MI0000060 (SEQ ID NO: 772) 
               
               
                 UGGGAUGAGGUAGUAGGUUGUAUAGUUUUAGGGUCACACCCACCACUGGGAGAUAACUAUACAAUCUACUGUCUUUC 
               
               
                 CUA 
               
               
                   
               
               
                 &gt;hsa-let-7a-2 MI0000061 (SEQ ID NO: 773) 
               
               
                 AGGUUGAGGUAGUAGGUUGUAUAGUUUAGAAUUACAUCAAGGGAGAUAACUGUACAGCCUCCUAGCUUUCCU 
               
               
                   
               
               
                 &gt;hsa-let-7a-3 MI0000062 (SEQ ID NO: 774) 
               
               
                 GGGUGAGGUAGUAGGUUGUAUAGUUUGGGGCUCUGCCCUGCUAUGGGAUAACUAUACAAUCUACUGUCUUUCCU 
               
               
                   
               
               
                 &gt;hsa-let-7b MI0000063 (SEQ ID NO: 775) 
               
               
                 CGGGGUGAGGUAGUAGGUUGUGUGGUUUCAGGGCAGUGAUGUUGCCCCUCGGAAGAUAACUAUACAACCUACUGCCU 
               
               
                 UCCCUG 
               
               
                   
               
               
                 &gt;hsa-let-7c MI0000064 (SEQ ID NO: 776) 
               
               
                 GCAUCCGGGUUGAGGUAGUAGGUUGUAUGGUUUAGAGUUACACCCUGGGAGUUAACUGUACAACCUUCUAGCUUUCC 
               
               
                 UUGGAGC 
               
               
                   
               
               
                 &gt;hsa-let-7d MI0000065 (SEQ ID NO: 777) 
               
               
                 CCUAGGAAGAGGUAGUAGGUUGCAUAGUUUUAGGGCAGGGAUUUUGCCCACAAGGAGGUAACUAUACGACCUGCUGC 
               
               
                 CUUUCUUAGG 
               
               
                   
               
               
                 &gt;hsa-let-7e MI0000066 (SEQ ID NO: 778) 
               
               
                 CCCGGGCUGAGGUAGGAGGUUGUAUAGUUGAGGAGGACACCCAAGGAGAUCACUAUACGGCCUCCUAGCUUUCCCCA 
               
               
                 GG 
               
               
                   
               
               
                 &gt;hsa-let-7f-1 MI0000067 (SEQ ID NO: 779) 
               
               
                 UCAGAGUGAGGUAGUAGAUUGUAUAGUUGUGGGGUAGUGAUUUUACCCUGUUCAGGAGAUAACUAUACAAUCUAUUG 
               
               
                 CCUUCCCUGA 
               
               
                   
               
               
                 &gt;hsa-let-7f-2 MI0000068 (SEQ ID NO: 780) 
               
               
                 UGUGGGAUGAGGUAGUAGAUUGUAUAGUUUUAGGGUCAUACCCCAUCUUGGAGAUAACUAUACAGUCUACUGUCUUU 
               
               
                 CCCACG 
               
               
                   
               
               
                 &gt;hsa-let-7g MI0000433 (SEQ ID NO: 781) 
               
               
                 AGGCUGAGGUAGUAGUUUGUACAGUUUGAGGGUCUAUGAUACCACCCGGUACAGGAGAUAACUGUACAGGCCACUGC 
               
               
                 CUUGCCA 
               
               
                   
               
               
                 &gt;hsa-let-7i MI0000434 (SEQ ID NO: 782) 
               
               
                 CUGGCUGAGGUAGUAGUUUGUGCUGUUGGUCGGGUUGUGACAUUGCCCGCUGUGGAGAUAACUGCGCAAGCUACUGC 
               
               
                 CUUGCUA 
               
               
                   
               
               
                 &gt;hsa-mir-1-1 MI0000651 (SEQ ID NO: 783) 
               
               
                 UGGGAAACAUACUUCUUUAUAUGCCCAUAUGGACCUGCUAAGCUAUGGAAUGUAAAGAAGUAUGUAUCUCA 
               
               
                   
               
               
                 &gt;hsa-mir-1-2 MI0000437 (SEQ ID NO: 784) 
               
               
                 ACCUACUCAGAGUACAUACUUCUUUAUGUACCCAUAUGAACAUACAAUGCUAUGGAAUGUAAAGAAGUAUGUAUUUU 
               
               
                 UGGUAGGC 
               
               
                   
               
               
                 &gt;hsa-mir-7-1 MI0000263 (SEQ ID NO: 785) 
               
               
                 UUGGAUGUUGGCCUAGUUCUGUGUGGAAGACUAGUGAUUUUGUUGUUUUUAGAUAACUAAAUCGACAACAAAUCACA 
               
               
                 GUCUGCCAUAUGGCACAGGCCAUGCCUCUACAG 
               
               
                   
               
               
                 &gt;hsa-mir-7-2 MI0000264 (SEQ ID NO: 786) 
               
               
                 CUGGAUACAGAGUGGACCGGCUGGCCCCAUCUGGAAGACUAGUGAUUUUGUUGUUGUCUUACUGCGCUCAACAACAA 
               
               
                 AUCCCAGUCUACCUAAUGGUGCCAGCCAUCGCA 
               
               
                   
               
               
                 &gt;hsa-mir-7-3 MI0000265 (SEQ ID NO: 787) 
               
               
                 AGAUUAGAGUGGCUGUGGUCUAGUGCUGUGUGGAAGACUAGUGAUUUUGUUGUUCUGAUGUACUACGACAACAAGUC 
               
               
                 ACAGCCGGCCUCAUAGCGCAGACUCCCUUCGAC 
               
               
                   
               
               
                 &gt;hsa-mir-9-1 MI0000466 (SEQ ID NO: 788) 
               
               
                 CGGGGUUGGUUGUUAUCUUUGGUUAUCUAGCUGUAUGAGUGGUGUGGAGUCUUCAUAAAGCUAGAUAACCGAAAGUA 
               
               
                 AAAAUAACCCCA 
               
               
                   
               
               
                 &gt;hsa-mir-9-2 MI0000467 (SEQ ID NO: 789) 
               
               
                 GGAAGCGAGUUGUUAUCUUUGGUUAUCUAGCUGUAUGAGUGUAUUGGUCUUCAUAAAGCUAGAUAACCGAAAGUAAA 
               
               
                 AACUCCUUCA 
               
               
                   
               
               
                 &gt;hsa-mir-9-3 MI0000468 (SEQ ID NO: 790) 
               
               
                 GGAGGCCCGUUUCUCUCUUUGGUUAUCUAGCUGUAUGAGUGCCACAGAGCCGUCAUAAAGCUAGAUAACCGAAAGUA 
               
               
                 GAAAUGAUUCUCA 
               
               
                   
               
               
                 &gt;hsa-mir-10a MI0000266 (SEQ ID NO: 791) 
               
               
                 GAUCUGUCUGUCUUCUGUAUAUACCCUGUAGAUCCGAAUUUGUGUAAGGAAUUUUGUGGUCACAAAUUCGUAUCUAG 
               
               
                 GGGAAUAUGUAGUUGACAUAAACACUCCGCUCU 
               
               
                   
               
               
                 &gt;hsa-mir-10b MI0000267 (SEQ ID NO: 792) 
               
               
                 CCAGAGGUUGUAACGUUGUCUAUAUAUACCCUGUAGAACCGAAUUUGUGUGGUAUCCGUAUAGUCACAGAUUCGAUU 
               
               
                 CUAGGGGAAUAUAUGGUCGAUGCAAAAACUUCA 
               
               
                   
               
               
                 &gt;hsa-mir-15a MI0000069 (SEQ ID NO: 793) 
               
               
                 CCUUGGAGUAAAGUAGCAGCACAUAAUGGUUUGUGGAUUUUGAAAAGGUGCAGGCCAUAUUGUGCUGCCUCAAAAAU 
               
               
                 ACAAGG 
               
               
                   
               
               
                 &gt;hsa-mir-15b MI0000438 (SEQ ID NO: 794) 
               
               
                 UUGAGGCCUUAAAGUACUGUAGCAGCACAUCAUGGUUUACAUGCUACAGUCAAGAUGCGAAUCAUUAUUUGCUGCUC 
               
               
                 UAGAAAUUUAAGGAAAUUCAU 
               
               
                   
               
               
                 &gt;hsa-mir-16-1 MI0000070 (SEQ ID NO: 795) 
               
               
                 GUCAGCAGUGCCUUAGCAGCACGUAAAUAUUGGCGUUAAGAUUCUAAAAUUAUCUCCAGUAUUAACUGUGCUGCUGA 
               
               
                 AGUAAGGUUGAC 
               
               
                   
               
               
                 &gt;hsa-mir-16-2 MI0000115 (SEQ ID NO: 796) 
               
               
                 GUUCCACUCUAGCAGCACGUAAAUAUUGGCGUAGUGAAAUAUAUAUUAAACACCAAUAUUACUGUGCUGCUUUAGUG 
               
               
                 UGAC 
               
               
                   
               
               
                 &gt;hsa-mir-17 MI0000071 (SEQ ID NO: 797) 
               
               
                 GUCAGAAUAAUGUCAAAGUGCUUACAGUGCAGGUAGUGAUAUGUGCAUCUACUGCAGUGAAGGCACUUGUAGCAUUA 
               
               
                 UGGUGAC 
               
               
                   
               
               
                 &gt;hsa-mir-18a MI0000072 (SEQ ID NO: 798) 
               
               
                 UGUUCUAAGGUGCAUCUAGUGCAGAUAGUGAAGUAGAUUAGCAUCUACUGCCCUAAGUGCUCCUUCUGGCA 
               
               
                   
               
               
                 &gt;hsa-mir-18b MI0001518 (SEQ ID NO: 799) 
               
               
                 UGUGUUAAGGUGCAUCUAGUGCAGUUAGUGAAGCAGCUUAGAAUCUACUGCCCUAAAUGCCCCUUCUGGCA 
               
               
                   
               
               
                 &gt;hsa-mir-19a MI0000073 (SEQ ID NO: 800) 
               
               
                 GCAGUCCUCUGUUAGUUUUGCAUAGUUGCACUACAAGAAGAAUGUAGUUGUGCAAAUCUAUGCAAAACUGAUGGUGG 
               
               
                 CCUGC 
               
               
                   
               
               
                 &gt;hsa-mir-19b-1 MI0000074 (SEQ ID NO: 801) 
               
               
                 CACUGUUCUAUGGUUAGUUUUGCAGGUUUGCAUCCAGCUGUGUGAUAUUCUGCUGUGCAAAUCCAUGCAAAACUGAC 
               
               
                 UGUGGUAGUG 
               
               
                   
               
               
                 &gt;hsa-mir-19b-2 MI0000075 (SEQ ID NO: 802) 
               
               
                 ACAUUGCUACUUACAAUUAGUUUUGCAGGUUUGCAUUUCAGCGUAUAUAUGUAUAUGUGGCUGUGCAAAUCCAUGCA 
               
               
                 AAACUGAUUGUGAUAAUGU 
               
               
                   
               
               
                 &gt;hsa-mir-20a MI0000076 (SEQ ID NO: 803) 
               
               
                 GUAGCACUAAAGUGCUUAUAGUGCAGGUAGUGUUUAGUUAUCUACUGCAUUAUGAGCACUUAAAGUACUGC 
               
               
                   
               
               
                 &gt;hsa-mir-20b MI0001519 (SEQ ID NO: 804) 
               
               
                 AGUACCAAAGUGCUCAUAGUGCAGGUAGUUUUGGCAUGACUCUACUGUAGUAUGGGCACUUCCAGUACU 
               
               
                   
               
               
                 &gt;hsa-mir-21 MI0000077 (SEQ ID NO: 805) 
               
               
                 UGUCGGGUAGCUUAUCAGACUGAUGUUGACUGUUGAAUCUCAUGGCAACACCAGUCGAUGGGCUGUCUGACA 
               
               
                   
               
               
                 &gt;hsa-mir-22 MI0000078 (SEQ ID NO: 806) 
               
               
                 GGCUGAGCCGCAGUAGUUCUUCAGUGGCAAGCUUUAUGUCCUGACCCAGCUAAAGCUGCCAGUUGAAGAACUGUUGC 
               
               
                 CCUCUGCC 
               
               
                   
               
               
                 &gt;hsa-mir-23a MI0000079 (SEQ ID NO: 807) 
               
               
                 GGCCGGCUGGGGUUCCUGGGGAUGGGAUUUGCUUCCUGUCACAAAUCACAUUGCCAGGGAUUUCCAACCGACC 
               
               
                   
               
               
                 &gt;hsa-mir-23b MI0000439 (SEQ ID NO: 808) 
               
               
                 CUCAGGUGCUCUGGCUGCUUGGGUUCCUGGCAUGCUGAUUUGUGACUUAAGAUUAAAAUCACAUUGCCAGGGAUUAC 
               
               
                 CACGCAACCACGACCUUGGC 
               
               
                   
               
               
                 &gt;hsa-mir-23c MI0016010 (SEQ ID NO: 809) 
               
               
                 AGUGACUUUCCAGGUGUCACACAGUGAGUGGCAUAAUCAGAGUACAAUUUGAGUCAUGCCCAUACAUCACAUUGCCA 
               
               
                 GUGAUUACCCAAGGAAAGUGACG 
               
               
                   
               
               
                 &gt;hsa-mir-24-1 MI0000080 (SEQ ID NO: 811) 
               
               
                 CUCCGGUGCCUACUGAGCUGAUAUCAGUUCUCAUUUUACACACUGGCUCAGUUCAGCAGGAACAGGAG 
               
               
                   
               
               
                 &gt;hsa-mir-24-2 MI0000081 (SEQ ID NO: 812) 
               
               
                 CUCUGCCUCCCGUGCCUACUGAGCUGAAACACAGUUGGUUUGUGUACACUGGCUCAGUUCAGCAGGAACAGGG 
               
               
                   
               
               
                 &gt;hsa-mir-25 MI0000082 (SEQ ID NO: 813) 
               
               
                 GGCCAGUGUUGAGAGGCGGAGACUUGGGCAAUUGCUGGACGCUGCCCUGGGCAUUGCACUUGUCUCGGUCUGACAGU 
               
               
                 GCCGGCC 
               
               
                   
               
               
                 &gt;hsa-mir-26a-1 MI0000083 (SEQ ID NO: 814) 
               
               
                 GUGGCCUCGUUCAAGUAAUCCAGGAUAGGCUGUGCAGGUCCCAAUGGGCCUAUUCUUGGUUACUUGCACGGGGACGC 
               
               
                   
               
               
                 &gt;hsa-mir-26a-2 MI0000750 (SEQ ID NO: 815) 
               
               
                 GGCUGUGGCUGGAUUCAAGUAAUCCAGGAUAGGCUGUUUCCAUCUGUGAGGCCUAUUCUUGAUUACUUGUUUCUGGA 
               
               
                 GGCAGCU 
               
               
                   
               
               
                 &gt;hsa-mir-26b MI0000084 (SEQ ID NO: 816) 
               
               
                 CCGGGACCCAGUUCAAGUAAUUCAGGAUAGGUUGUGUGCUGUCCAGCCUGUUCUCCAUUACUUGGCUCGGGGACCGG 
               
               
                   
               
               
                 &gt;hsa-mir-27a MI0000085 (SEQ ID NO: 817) 
               
               
                 CUGAGGAGCAGGGCUUAGCUGCUUGUGAGCAGGGUCCACACCAAGUCGUGUUCACAGUGGCUAAGUUCCGCCCCCCAG 
               
               
                   
               
               
                 &gt;hsa-mir-27b MI0000440 (SEQ ID NO: 817) 
               
               
                 ACCUCUCUAACAAGGUGCAGAGCUUAGCUGAUUGGUGAACAGUGAUUGGUUUCCGCUUUGUUCACAGUGGCUAAGUU 
               
               
                 CUGCACCUGAAGAGAAGGUG 
               
               
                   
               
               
                 &gt;hsa-mir-28 MI0000086 (SEQ ID NO: 818) 
               
               
                 GGUCCUUGCCCUCAAGGAGCUCACAGUCUAUUGAGUUACCUUUCUGACUUUCCCACUAGAUUGUGAGCUCCUGGAGG 
               
               
                 GCAGGCACU 
               
               
                   
               
               
                 &gt;hsa-mir-29a MI0000087 (SEQ ID NO: 819) 
               
               
                 AUGACUGAUUUCUUUUGGUGUUCAGAGUCAAUAUAAUUUUCUAGCACCAUCUGAAAUCGGUUAU 
               
               
                   
               
               
                 &gt;hsa-mir-29b-1 MI0000105 (SEQ ID NO: 820) 
               
               
                 CUUCAGGAAGCUGGUUUCAUAUGGUGGUUUAGAUUUAAAUAGUGAUUGUCUAGCACCAUUUGAAAUCAGUGUUCUUG 
               
               
                 GGGG 
               
               
                   
               
               
                 &gt;hsa-mir-29b-2 MI0000107 (SEQ ID NO: 821) 
               
               
                 CUUCUGGAAGCUGGUUUCACAUGGUGGCUUAGAUUUUUCCAUCUUUGUAUCUAGCACCAUUUGAAAUCAGUGUUUUA 
               
               
                 GGAG 
               
               
                   
               
               
                 &gt;hsa-mir-29c MI0000735 (SEQ ID NO: 822) 
               
               
                 AUCUCUUACACAGGCUGACCGAUUUCUCCUGGUGUUCAGAGUCUGUUUUUGUCUAGCACCAUUUGAAAUCGGUUAUG 
               
               
                 AUGUAGGGGGA 
               
               
                   
               
               
                 &gt;hsa-mir-30a MI0000088 (SEQ ID NO: 823) 
               
               
                 GCGACUGUAAACAUCCUCGACUGGAAGCUGUGAAGCCACAGAUGGGCUUUCAGUCGGAUGUUUGCAGCUGC 
               
               
                   
               
               
                 &gt;hsa-mir-30b MI0000441 (SEQ ID NO: 824) 
               
               
                 ACCAAGUUUCAGUUCAUGUAAACAUCCUACACUCAGCUGUAAUACAUGGAUUGGCUGGGAGGUGGAUGUUUACUUCA 
               
               
                 GCUGACUUGGA 
               
               
                   
               
               
                 &gt;hsa-mir-30c-1 MI0000736 (SEQ ID NO: 825) 
               
               
                 ACCAUGCUGUAGUGUGUGUAAACAUCCUACACUCUCAGCUGUGAGCUCAAGGUGGCUGGGAGAGGGUUGUUUACUCC 
               
               
                 UUCUGCCAUGGA 
               
               
                   
               
               
                 &gt;hsa-mir-30c-2 MI0000254 (SEQ ID NO: 826) 
               
               
                 AGAUACUGUAAACAUCCUACACUCUCAGCUGUGGAAAGUAAGAAAGCUGGGAGAAGGCUGUUUACUCUUUCU 
               
               
                   
               
               
                 &gt;hsa-mir-30d MI0000255 (SEQ ID NO: 827) 
               
               
                 GUUGUUGUAAACAUCCCCGACUGGAAGCUGUAAGACACAGCUAAGCUUUCAGUCAGAUGUUUGCUGCUAC 
               
               
                   
               
               
                 &gt;hsa-mir-30e MI0000749 (SEQ ID NO: 828) 
               
               
                 GGGCAGUCUUUGCUACUGUAAACAUCCUUGACUGGAAGCUGUAAGGUGUUCAGAGGAGCUUUCAGUCGGAUGUUUAC 
               
               
                 AGCGGCAGGCUGCCA 
               
               
                   
               
               
                 &gt;hsa-mir-31 MI0000089 (SEQ ID NO: 829) 
               
               
                 GGAGAGGAGGCAAGAUGCUGGCAUAGCUGUUGAACUGGGAACCUGCUAUGCCAACAUAUUGCCAUCUUUCC 
               
               
                   
               
               
                 &gt;hsa-mir-32 MI0000090 (SEQ ID NO: 830) 
               
               
                 GGAGAUAUUGCACAUUACUAAGUUGCAUGUUGUCACGGCCUCAAUGCAAUUUAGUGUGUGUGAUAUUUUC 
               
               
                   
               
               
                 &gt;hsa-mir-33a MI0000091 (SEQ ID NO: 831) 
               
               
                 CUGUGGUGCAUUGUAGUUGCAUUGCAUGUUCUGGUGGUACCCAUGCAAUGUUUCCACAGUGCAUCACAG 
               
               
                   
               
               
                 &gt;hsa-mir-33b MI0003646 (SEQ ID NO: 832) 
               
               
                 GCGGGCGGCCCCGCGGUGCAUUGCUGUUGCAUUGCACGUGUGUGAGGCGGGUGCAGUGCCUCGGCAGUGCAGCCCGG 
               
               
                 AGCCGGCCCCUGGCACCAC 
               
               
                   
               
               
                 &gt;hsa-mir-34a MI0000268 (SEQ ID NO: 833) 
               
               
                 GGCCAGCUGUGAGUGUUUCUUUGGCAGUGUCUUAGCUGGUUGUUGUGAGCAAUAGUAAGGAAGCAAUCAGCAAGUAU 
               
               
                 ACUGCCCUAGAAGUGCUGCACGUUGUGGGGCCC 
               
               
                   
               
               
                 &gt;hsa-mir-34b MI0000742 (SEQ ID NO: 834) 
               
               
                 GUGCUCGGUUUGUAGGCAGUGUCAUUAGCUGAUUGUACUGUGGUGGUUACAAUCACUAACUCCACUGCCAUCAAAAC 
               
               
                 AAGGCAC 
               
               
                   
               
               
                 &gt;hsa-mir-34c MI0000743 (SEQ ID NO: 835) 
               
               
                 AGUCUAGUUACUAGGCAGUGUAGUUAGCUGAUUGCUAAUAGUACCAAUCACUAACCACACGGCCAGGUAAAAAGAUU 
               
               
                   
               
               
                 &gt;hsa-mir-92a-1 MI0000093 (SEQ ID NO: 836) 
               
               
                 CUUUCUACACAGGUUGGGAUCGGUUGCAAUGCUGUGUUUCUGUAUGGUAUUGCACUUGUCCCGGCCUGUUGAGUUUGG 
               
               
                   
               
               
                 &gt;hsa-mir-92a-2 MI0000094 (SEQ ID NO: 837) 
               
               
                 UCAUCCCUGGGUGGGGAUUUGUUGCAUUACUUGUGUUCUAUAUAAAGUAUUGCACUUGUCCCGGCCUGUGGAAGA 
               
               
                   
               
               
                 &gt;hsa-mir-92b MI0003560 (SEQ ID NO: 838) 
               
               
                 CGGGCCCCGGGCGGGCGGGAGGGACGGGACGCGGUGCAGUGUUGUUUUUUCCCCCGCCAAUAUUGCACUCGUCCCGG 
               
               
                 CCUCCGGCCCCCCCGGCCC 
               
               
                   
               
               
                 &gt;hsa-mir-93 MI0000095 (SEQ ID NO: 839) 
               
               
                 CUGGGGGCUCCAAAGUGCUGUUCGUGCAGGUAGUGUGAUUACCCAACCUACUGCUGAGCUAGCACUUCCCGAGCCCC 
               
               
                 CGG 
               
               
                   
               
               
                 &gt;hsa-mir-95 MI0000097 (SEQ ID NO: 840) 
               
               
                 AACACAGUGGGCACUCAAUAAAUGUCUGUUGAAUUGAAAUGCGUUACAUUCAACGGGUAUUUAUUGAGCACCCACUC 
               
               
                 UGUG 
               
               
                   
               
               
                 &gt;hsa-mir-96 MI0000098 (SEQ ID NO: 841) 
               
               
                 UGGCCGAUUUUGGCACUAGCACAUUUUUGCUUGUGUCUCUCCGCUCUGAGCAAUCAUGUGCAGUGCCAAUAUGGGAAA 
               
               
                   
               
               
                 &gt;hsa-mir-98 MI0000100 (SEQ ID NO: 842) 
               
               
                 AGGAUUCUGCUCAUGCCAGGGUGAGGUAGUAAGUUGUAUUGUUGUGGGGUAGGGAUAUUAGGCCCCAAUUAGAAGAU 
               
               
                 AACUAUACAACUUACUACUUUCCCUGGUGUGUGGCAUAUUCA 
               
               
                   
               
               
                 &gt;hsa-mir-99a MI0000101 (SEQ ID NO: 843) 
               
               
                 CCCAUUGGCAUAAACCCGUAGAUCCGAUCUUGUGGUGAAGUGGACCGCACAAGCUCGCUUCUAUGGGUCUGUGUCAG 
               
               
                 UGUG 
               
               
                   
               
               
                 &gt;hsa-mir-99b MI0000746 (SEQ ID NO: 844) 
               
               
                 GGCACCCACCCGUAGAACCGACCUUGCGGGGCCUUCGCCGCACACAAGCUCGUGUCUGUGGGUCCGUGUC 
               
               
                   
               
               
                 &gt;hsa-mir-100 MI0000102 (SEQ ID NO: 845) 
               
               
                 CCUGUUGCCACAAACCCGUAGAUCCGAACUUGUGGUAUUAGUCCGCACAAGCUUGUAUCUAUAGGUAUGUGUCUGUU 
               
               
                 AGG 
               
               
                   
               
               
                 &gt;hsa-mir-101-1 MI0000103 (SEQ ID NO: 846) 
               
               
                 UGCCCUGGCUCAGUUAUCACAGUGCUGAUGCUGUCUAUUCUAAAGGUACAGUACUGUGAUAACUGAAGGAUGGCA 
               
               
                   
               
               
                 &gt;hsa-mir-101-2 MI0000739 (SEQ ID NO: 847) 
               
               
                 ACUGUCCUUUUUCGGUUAUCAUGGUACCGAUGCUGUAUAUCUGAAAGGUACAGUACUGUGAUAACUGAAGAAUGGUG 
               
               
                 GU 
               
               
                   
               
               
                 &gt;hsa-mir-103a-1 MI0000109 (SEQ ID NO: 848) 
               
               
                 UACUGCCCUCGGCUUCUUUACAGUGCUGCCUUGUUGCAUAUGGAUCAAGCAGCAUUGUACAGGGCUAUGAAGGCAUUG 
               
               
                   
               
               
                 &gt;hsa-mir-103a-2 MI0000108 (SEQ ID NO: 849) 
               
               
                 UUGUGCUUUCAGCUUCUUUACAGUGCUGCCUUGUAGCAUUCAGGUCAAGCAGCAUUGUACAGGGCUAUGAAAGAACCA 
               
               
                   
               
               
                 &gt;hsa-mir-103b-1 MI0007261 (SEQ ID NO: 850) 
               
               
                 UCAUAGCCCUGUACAAUGCUGCUUGAUCCAUAUGCAACAAGGCAGCACUGUAAAGAAGCCGA 
               
               
                   
               
               
                 &gt;hsa-mir-103b-2 MI0007262 (SEQ ID NO: 851) 
               
               
                 UCAUAGCCCUGUACAAUGCUGCUUGACCUGAAUGCUACAAGGCAGCACUGUAAAGAAGCUGA 
               
               
                   
               
               
                 &gt;hsa-mir-105-1 MI0000111 (SEQ ID NO: 852) 
               
               
                 UGUGCAUCGUGGUCAAAUGCUCAGACUCCUGUGGUGGCUGCUCAUGCACCACGGAUGUUUGAGCAUGUGCUACGGUG 
               
               
                 UCUA 
               
               
                   
               
               
                 &gt;hsa-mir-105-2 MI0000112 (SEQ ID NO: 853) 
               
               
                 UGUGCAUCGUGGUCAAAUGCUCAGACUCCUGUGGUGGCUGCUUAUGCACCACGGAUGUUUGAGCAUGUGCUAUGGUG 
               
               
                 UCUA 
               
               
                   
               
               
                 &gt;hsa-mir-106a MI0000113 (SEQ ID NO: 854) 
               
               
                 CCUUGGCCAUGUAAAAGUGCUUACAGUGCAGGUAGCUUUUUGAGAUCUACUGCAAUGUAAGCACUUCUUACAUUACC 
               
               
                 AUGG 
               
               
                   
               
               
                 &gt;hsa-mir-106b MI0000734 (SEQ ID NO: 855) 
               
               
                 CCUGCCGGGGCUAAAGUGCUGACAGUGCAGAUAGUGGUCCUCUCCGUGCUACCGCACUGUGGGUACUUGCUGCUCCA 
               
               
                 GCAGG 
               
               
                   
               
               
                 &gt;hsa-mir-107 MI0000114 (SEQ ID NO: 856) 
               
               
                 CUCUCUGCUUUCAGCUUCUUUACAGUGUUGCCUUGUGGCAUGGAGUUCAAGCAGCAUUGUACAGGGCUAUCAAAGCA 
               
               
                 CAGA 
               
               
                   
               
               
                 &gt;hsa-mir-122 MI0000442 (SEQ ID NO: 857) 
               
               
                 CCUUAGCAGAGCUGUGGAGUGUGACAAUGGUGUUUGUGUCUAAACUAUCAAACGCCAUUAUCACACUAAAUAGCUAC 
               
               
                 UGCUAGGC 
               
               
                   
               
               
                 &gt;hsa-mir-124-1 MI0000443 (SEQ ID NO: 858) 
               
               
                 AGGCCUCUCUCUCCGUGUUCACAGCGGACCUUGAUUUAAAUGUCCAUACAAUUAAGGCACGCGGUGAAUGCCAAGAA 
               
               
                 UGGGGCUG 
               
               
                   
               
               
                 &gt;hsa-mir-124-2 MI0000444 (SEQ ID NO: 859) 
               
               
                 AUCAAGAUUAGAGGCUCUGCUCUCCGUGUUCACAGCGGACCUUGAUUUAAUGUCAUACAAUUAAGGCACGCGGUGAA 
               
               
                 UGCCAAGAGCGGAGCCUACGGCUGCACUUGAA 
               
               
                   
               
               
                 &gt;hsa-mir-124-3 MI0000445 (SEQ ID NO: 860) 
               
               
                 UGAGGGCCCCUCUGCGUGUUCACAGCGGACCUUGAUUUAAUGUCUAUACAAUUAAGGCACGCGGUGAAUGCCAAGAG 
               
               
                 AGGCGCCUCC 
               
               
                   
               
               
                 &gt;hsa-mir-125a MI0000469 (SEQ ID NO: 861) 
               
               
                 UGCCAGUCUCUAGGUCCCUGAGACCCUUUAACCUGUGAGGACAUCCAGGGUCACAGGUGAGGUUCUUGGGAGCCUGG 
               
               
                 CGUCUGGCC 
               
               
                   
               
               
                 &gt;hsa-mir-125b-1 MI0000446 (SEQ ID NO: 862) 
               
               
                 UGCGCUCCUCUCAGUCCCUGAGACCCUAACUUGUGAUGUUUACCGUUUAAAUCCACGGGUUAGGCUCUUGGGAGCUG 
               
               
                 CGAGUCGUGCU 
               
               
                   
               
               
                 &gt;hsa-mir-125b-2 MI0000470 (SEQ ID NO: 863) 
               
               
                 ACCAGACUUUUCCUAGUCCCUGAGACCCUAACUUGUGAGGUAUUUUAGUAACAUCACAAGUCAGGCUCUUGGGACCU 
               
               
                 AGGCGGAGGGGA 
               
               
                   
               
               
                 &gt;hsa-mir-126 MI0000471 (SEQ ID NO: 864) 
               
               
                 CGCUGGCGACGGGACAUUAUUACUUUUGGUACGCGCUGUGACACUUCAAACUCGUACCGUGAGUAAUAAUGCGCCGU 
               
               
                 CCACGGCA 
               
               
                   
               
               
                 &gt;hsa-mir-127 MI0000472 (SEQ ID NO: 865) 
               
               
                 UGUGAUCACUGUCUCCAGCCUGCUGAAGCUCAGAGGGCUCUGAUUCAGAAAGAUCAUCGGAUCCGUCUGAGCUUGGC 
               
               
                 UGGUCGGAAGUCUCAUCAUC 
               
               
                   
               
               
                 &gt;hsa-mir-128-1 MI0000447 (SEQ ID NO: 866) 
               
               
                 UGAGCUGUUGGAUUCGGGGCCGUAGCACUGUCUGAGAGGUUUACAUUUCUCACAGUGAACCGGUCUCUUUUUCAGCU 
               
               
                 GCUUC 
               
               
                   
               
               
                 &gt;hsa-mir-128-2 MI0000727 (SEQ ID NO: 867) 
               
               
                 UGUGCAGUGGGAAGGGGGGCCGAUACACUGUACGAGAGUGAGUAGCAGGUCUCACAGUGAACCGGUCUCUUUCCCUA 
               
               
                 CUGUGUC 
               
               
                   
               
               
                 &gt;hsa-mir-129-1 MI0000252 (SEQ ID NO: 868) 
               
               
                 GGAUCUUUUUGCGGUCUGGGCUUGCUGUUCCUCUCAACAGUAGUCAGGAAGCCCUUACCCCAAAAAGUAUCU 
               
               
                   
               
               
                 &gt;hsa-mir-129-2 MI0000473 (SEQ ID NO: 869) 
               
               
                 UGCCCUUCGCGAAUCUUUUUGCGGUCUGGGCUUGCUGUACAUAACUCAAUAGCCGGAAGCCCUUACCCCAAAAAGCA 
               
               
                 UUUGCGGAGGGCG 
               
               
                   
               
               
                 &gt;hsa-mir-130a MI0000448 (SEQ ID NO: 870) 
               
               
                 UGCUGCUGGCCAGAGCUCUUUUCACAUUGUGCUACUGUCUGCACCUGUCACUAGCAGUGCAAUGUUAAAAGGGCAUU 
               
               
                 GGCCGUGUAGUG 
               
               
                   
               
               
                 &gt;hsa-mir-130b MI0000748 (SEQ ID NO: 871) 
               
               
                 GGCCUGCCCGACACUCUUUCCCUGUUGCACUACUAUAGGCCGCUGGGAAGCAGUGCAAUGAUGAAAGGGCAUCGGUC 
               
               
                 AGGUC 
               
               
                   
               
               
                 &gt;hsa-mir-132 MI0000449 (SEQ ID NO: 872) 
               
               
                 CCGCCCCCGCGUCUCCAGGGCAACCGUGGCUUUCGAUUGUUACUGUGGGAACUGGAGGUAACAGUCUACAGCCAUGG 
               
               
                 UCGCCCCGCAGCACGCCCACGCGC 
               
               
                   
               
               
                 &gt;hsa-mir-133a-1 MI0000450 (SEQ ID NO: 873) 
               
               
                 ACAAUGCUUUGCUAGAGCUGGUAAAAUGGAACCAAAUCGCCUCUUCAAUGGAUUUGGUCCCCUUCAACCAGCUGUAG 
               
               
                 CUAUGCAUUGA 
               
               
                   
               
               
                 &gt;hsa-mir-133a-2 MI0000451 (SEQ ID NO: 874) 
               
               
                 GGGAGCCAAAUGCUUUGCUAGAGCUGGUAAAAUGGAACCAAAUCGACUGUCCAAUGGAUUUGGUCCCCUUCAACCAG 
               
               
                 CUGUAGCUGUGCAUUGAUGGCGCCG 
               
               
                   
               
               
                 &gt;hsa-mir-133b MI0000822 (SEQ ID NO: 875) 
               
               
                 CCUCAGAAGAAAGAUGCCCCCUGCUCUGGCUGGUCAAACGGAACCAAGUCCGUCUUCCUGAGAGGUUUGGUCCCCUU 
               
               
                 CAACCAGCUACAGCAGGGCUGGCAAUGCCCAGUCCUUGGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-134 MI0000474 (SEQ ID NO: 876) 
               
               
                 CAGGGUGUGUGACUGGUUGACCAGAGGGGCAUGCACUGUGUUCACCCUGUGGGCCACCUAGUCACCAACCCUC 
               
               
                   
               
               
                 &gt;hsa-mir-135a-1 MI0000452 (SEQ ID NO: 877) 
               
               
                 AGGCCUCGCUGUUCUCUAUGGCUUUUUAUUCCUAUGUGAUUCUACUGCUCACUCAUAUAGGGAUUGGAGCCGUGGCG 
               
               
                 CACGGCGGGGACA 
               
               
                   
               
               
                 &gt;hsa-mir-135a-2 MI0000453 (SEQ ID NO: 878) 
               
               
                 AGAUAAAUUCACUCUAGUGCUUUAUGGCUUUUUAUUCCUAUGUGAUAGUAAUAAAGUCUCAUGUAGGGAUGGAAGCC 
               
               
                 AUGAAAUACAUUGUGAAAAAUCA 
               
               
                   
               
               
                 &gt;hsa-mir-135b MI0000810 (SEQ ID NO: 879) 
               
               
                 CACUCUGCUGUGGCCUAUGGCUUUUCAUUCCUAUGUGAUUGCUGUCCCAAACUCAUGUAGGGCUAAAAGCCAUGGGC 
               
               
                 UACAGUGAGGGGCGAGCUCC 
               
               
                   
               
               
                 &gt;hsa-mir-136 MI0000475 (SEQ ID NO: 880) 
               
               
                 UGAGCCCUCGGAGGACUCCAUUUGUUUUGAUGAUGGAUUCUUAUGCUCCAUCAUCGUCUCAAAUGAGUCUUCAGAGG 
               
               
                 GUUCU 
               
               
                   
               
               
                 &gt;hsa-mir-137 MI0000454 (SEQ ID NO: 881) 
               
               
                 GGUCCUCUGACUCUCUUCGGUGACGGGUAUUCUUGGGUGGAUAAUACGGAUUACGUUGUUAUUGCUUAAGAAUACGC 
               
               
                 GUAGUCGAGGAGAGUACCAGCGGCA 
               
               
                   
               
               
                 &gt;hsa-mir-138-1 MI0000476 (SEQ ID NO: 882) 
               
               
                 CCCUGGCAUGGUGUGGUGGGGCAGCUGGUGUUGUGAAUCAGGCCGUUGCCAAUCAGAGAACGGCUACUUCACAACAC 
               
               
                 CAGGGCCACACCACACUACAGG 
               
               
                   
               
               
                 &gt;hsa-mir-138-2 MI0000455 (SEQ ID NO: 883) 
               
               
                 CGUUGCUGCAGCUGGUGUUGUGAAUCAGGCCGACGAGCAGCGCAUCCUCUUACCCGGCUAUUUCACGACACCAGGGU 
               
               
                 UGCAUCA 
               
               
                   
               
               
                 &gt;hsa-mir-139 MI0000261 (SEQ ID NO: 884) 
               
               
                 GUGUAUUCUACAGUGCACGUGUCUCCAGUGUGGCUCGGAGGCUGGAGACGCGGCCCUGUUGGAGUAAC 
               
               
                   
               
               
                 &gt;hsa-mir-140 MI0000456 (SEQ ID NO: 885) 
               
               
                 UGUGUCUCUCUCUGUGUCCUGCCAGUGGUUUUACCCUAUGGUAGGUUACGUCAUGCUGUUCUACCACAGGGUAGAAC 
               
               
                 CACGGACAGGAUACCGGGGCACC 
               
               
                   
               
               
                 &gt;hsa-mir-141 MI0000457 (SEQ ID NO: 886) 
               
               
                 CGGCCGGCCCUGGGUCCAUCUUCCAGUACAGUGUUGGAUGGUCUAAUUGUGAAGCUCCUAACACUGUCUGGUAAAGA 
               
               
                 UGGCUCCCGGGUGGGUUC 
               
               
                   
               
               
                 &gt;hsa-mir-142 MI0000458 (SEQ ID NO: 887) 
               
               
                 GACAGUGCAGUCACCCAUAAAGUAGAAAGCACUACUAACAGCACUGGAGGGUGUAGUGUUUCCUACUUUAUGGAUGA 
               
               
                 GUGUACUGUG 
               
               
                   
               
               
                 &gt;hsa-mir-143 MI0000459 (SEQ ID NO: 888) 
               
               
                 GCGCAGCGCCCUGUCUCCCAGCCUGAGGUGCAGUGCUGCAUCUCUGGUCAGUUGGGAGUCUGAGAUGAAGCACUGUA 
               
               
                 GCUCAGGAAGAGAGAAGUUGUUCUGCAGC 
               
               
                   
               
               
                 &gt;hsa-mir-144 MI0000460 (SEQ ID NO: 889) 
               
               
                 UGGGGCCCUGGCUGGGAUAUCAUCAUAUACUGUAAGUUUGCGAUGAGACACUACAGUAUAGAUGAUGUACUAGUCCG 
               
               
                 GGCACCCCC 
               
               
                   
               
               
                 &gt;hsa-mir-145 MI0000461 (SEQ ID NO: 890) 
               
               
                 CACCUUGUCCUCACGGUCCAGUUUUCCCAGGAAUCCCUUAGAUGCUAAGAUGGGGAUUCCUGGAAAUACUGUUCUUG 
               
               
                 AGGUCAUGGUU 
               
               
                   
               
               
                 &gt;hsa-mir-146a MI0000477 (SEQ ID NO: 891) 
               
               
                 CCGAUGUGUAUCCUCAGCUUUGAGAACUGAAUUCCAUGGGUUGUGUCAGUGUCAGACCUCUGAAAUUCAGUUCUUCA 
               
               
                 GCUGGGAUAUCUCUGUCAUCGU 
               
               
                   
               
               
                 &gt;hsa-mir-146b MI0003129 (SEQ ID NO: 892) 
               
               
                 CCUGGCACUGAGAACUGAAUUCCAUAGGCUGUGAGCUCUAGCAAUGCCCUGUGGACUCAGUUCUGGUGCCCGG 
               
               
                   
               
               
                 &gt;hsa-mir-147a MI0000262 (SEQ ID NO: 893) 
               
               
                 AAUCUAAAGACAACAUUUCUGCACACACACCAGACUAUGGAAGCCAGUGUGUGGAAAUGCUUCUGCUAGAUU 
               
               
                   
               
               
                 &gt;hsa-mir-147b MI0005544 (SEQ ID NO: 894) 
               
               
                 UAUAAAUCUAGUGGAAACAUUUCUGCACAAACUAGAUUCUGGACACCAGUGUGCGGAAAUGCUUCUGCUACAUUUUU 
               
               
                 AGG 
               
               
                   
               
               
                 &gt;hsa-mir-148a MI0000253 (SEQ ID NO: 895) 
               
               
                 GAGGCAAAGUUCUGAGACACUCCGACUCUGAGUAUGAUAGAAGUCAGUGCACUACAGAACUUUGUCUC 
               
               
                   
               
               
                 &gt;hsa-mir-148b MI0000811 (SEQ ID NO: 896) 
               
               
                 CAAGCACGAUUAGCAUUUGAGGUGAAGUUCUGUUAUACACUCAGGCUGUGGCUCUCUGAAAGUCAGUGCAUCACAGA 
               
               
                 ACUUUGUCUCGAAAGCUUUCUA 
               
               
                   
               
               
                 &gt;hsa-mir-149 MI0000478 (SEQ ID NO: 897) 
               
               
                 GCCGGCGCCCGAGCUCUGGCUCCGUGUCUUCACUCCCGUGCUUGUCCGAGGAGGGAGGGAGGGACGGGGGCUGUGCU 
               
               
                 GGGGCAGCUGGA 
               
               
                   
               
               
                 &gt;hsa-mir-150 MI0000479 (SEQ ID NO: 898) 
               
               
                 CUCCCCAUGGCCCUGUCUCCCAACCCUUGUACCAGUGCUGGGCUCAGACCCUGGUACAGGCCUGGGGGACAGGGACC 
               
               
                 UGGGGAC 
               
               
                   
               
               
                 &gt;hsa-mir-151a MI0000809 (SEQ ID NO: 899) 
               
               
                 UUUCCUGCCCUCGAGGAGCUCACAGUCUAGUAUGUCUCAUCCCCUACUAGACUGAAGCUCCUUGAGGACAGGGAUGG 
               
               
                 UCAUACUCACCUC 
               
               
                   
               
               
                 &gt;hsa-mir-151b MI0003772 (SEQ ID NO: 900) 
               
               
                 ACCUCUGAUGUGUCAGUCUCUCUUCAGGGCUCCCGAGACACAGAAACAGACACCUGCCCUCGAGGAGCUCACAGUCU 
               
               
                 AGACAAACAAACCCAGGGU 
               
               
                   
               
               
                 &gt;hsa-mir-152 MI0000462 (SEQ ID NO: 901) 
               
               
                 UGUCCCCCCCGGCCCAGGUUCUGUGAUACACUCCGACUCGGGCUCUGGAGCAGUCAGUGCAUGACAGAACUUGGGCC 
               
               
                 CGGAAGGACC 
               
               
                   
               
               
                 &gt;hsa-mir-153-1 MI0000463 (SEQ ID NO: 902) 
               
               
                 CUCACAGCUGCCAGUGUCAUUUUUGUGAUCUGCAGCUAGUAUUCUCACUCCAGUUGCAUAGUCACAAAAGUGAUCAU 
               
               
                 UGGCAGGUGUGGC 
               
               
                   
               
               
                 &gt;hsa-mir-153-2 MI0000464 (SEQ ID NO: 903) 
               
               
                 AGCGGUGGCCAGUGUCAUUUUUGUGAUGUUGCAGCUAGUAAUAUGAGCCCAGUUGCAUAGUCACAAAAGUGAUCAUU 
               
               
                 GGAAACUGUG 
               
               
                   
               
               
                 &gt;hsa-mir-154 MI0000480 (SEQ ID NO: 904) 
               
               
                 GUGGUACUUGAAGAUAGGUUAUCCGUGUUGCCUUCGCUUUAUUUGUGACGAAUCAUACACGGUUGACCUAUUUUUCA 
               
               
                 GUACCAA 
               
               
                   
               
               
                 &gt;hsa-mir-155 MI0000681 (SEQ ID NO: 905) 
               
               
                 CUGUUAAUGCUAAUCGUGAUAGGGGUUUUUGCCUCCAACUGACUCCUACAUAUUAGCAUUAACAG 
               
               
                   
               
               
                 &gt;hsa-mir-181a-1 MI0000289 (SEQ ID NO: 906) 
               
               
                 UGAGUUUUGAGGUUGCUUCAGUGAACAUUCAACGCUGUCGGUGAGUUUGGAAUUAAAAUCAAAACCAUCGACCGUUG 
               
               
                 AUUGUACCCUAUGGCUAACCAUCAUCUACUCCA 
               
               
                   
               
               
                 &gt;hsa-mir-181a-2 MI0000269 (SEQ ID NO: 907) 
               
               
                 AGAAGGGCUAUCAGGCCAGCCUUCAGAGGACUCCAAGGAACAUUCAACGCUGUCGGUGAGUUUGGGAUUUGAAAAAA 
               
               
                 CCACUGACCGUUGACUGUACCUUGGGGUCCUUA 
               
               
                   
               
               
                 &gt;hsa-mir-181b-1 MI0000270 (SEQ ID NO: 908) 
               
               
                 CCUGUGCAGAGAUUAUUUUUUAAAAGGUCACAAUCAACAUUCAUUGCUGUCGGUGGGUUGAACUGUGUGGACAAGCU 
               
               
                 CACUGAACAAUGAAUGCAACUGUGGCCCCGCUU 
               
               
                   
               
               
                 &gt;hsa-mir-181b-2 MI0000683 (SEQ ID NO: 909) 
               
               
                 CUGAUGGCUGCACUCAACAUUCAUUGCUGUCGGUGGGUUUGAGUCUGAAUCAACUCACUGAUCAAUGAAUGCAAACU 
               
               
                 GCGGACCAAACA 
               
               
                   
               
               
                 &gt;hsa-mir-181c MI0000271 (SEQ ID NO: 910) 
               
               
                 CGGAAAAUUUGCCAAGGGUUUGGGGGAACAUUCAACCUGUCGGUGAGUUUGGGCAGCUCAGGCAAACCAUCGACCGU 
               
               
                 UGAGUGGACCCUGAGGCCUGGAAUUGCCAUCCU 
               
               
                   
               
               
                 &gt;hsa-mir-181d MI0003139 (SEQ ID NO: 911) 
               
               
                 GUCCCCUCCCCUAGGCCACAGCCGAGGUCACAAUCAACAUUCAUUGUUGUCGGUGGGUUGUGAGGACUGAGGCCAGA 
               
               
                 CCCACCGGGGGAUGAAUGUCACUGUGGCUGGGCCAGACACGGCUUAAGGGGAAUGGGGAC 
               
               
                   
               
               
                 &gt;hsa-mir-182 MI0000272 (SEQ ID NO: 912) 
               
               
                 GAGCUGCUUGCCUCCCCCCGUUUUUGGCAAUGGUAGAACUCACACUGGUGAGGUAACAGGAUCCGGUGGUUCUAGAC 
               
               
                 UUGCCAACUAUGGGGCGAGGACUCAGCCGGCAC 
               
               
                   
               
               
                 &gt;hsa-mir-183 MI0000273 (SEQ ID NO: 913) 
               
               
                 CCGCAGAGUGUGACUCCUGUUCUGUGUAUGGCACUGGUAGAAUUCACUGUGAACAGUCUCAGUCAGUGAAUUACCGA 
               
               
                 AGGGCCAUAAACAGAGCAGAGACAGAUCCACGA 
               
               
                   
               
               
                 &gt;hsa-mir-184 MI0000481 (SEQ ID NO: 914) 
               
               
                 CCAGUCACGUCCCCUUAUCACUUUUCCAGCCCAGCUUUGUGACUGUAAGUGUUGGACGGAGAACUGAUAAGGGUAGG 
               
               
                 UGAUUGA 
               
               
                   
               
               
                 &gt;hsa-mir-185 MI0000482 (SEQ ID NO: 915) 
               
               
                 AGGGGGCGAGGGAUUGGAGAGAAAGGCAGUUCCUGAUGGUCCCCUCCCCAGGGGCUGGCUUUCCUCUGGUCCUUCCC 
               
               
                 UCCCA 
               
               
                   
               
               
                 &gt;hsa-mir-186 MI0000483 (SEQ ID NO: 916) 
               
               
                 UGCUUGUAACUUUCCAAAGAAUUCUCCUUUUGGGCUUUCUGGUUUUAUUUUAAGCCCAAAGGUGAAUUUUUUGGGAA 
               
               
                 GUUUGAGCU 
               
               
                   
               
               
                 &gt;hsa-mir-187 MI0000274 (SEQ ID NO: 917) 
               
               
                 GGUCGGGCUCACCAUGACACAGUGUGAGACCUCGGGCUACAACACAGGACCCGGGCGCUGCUCUGACCCCUCGUGUC 
               
               
                 UUGUGUUGCAGCCGGAGGGACGCAGGUCCGCA 
               
               
                   
               
               
                 &gt;hsa-mir-188 MI0000484 (SEQ ID NO: 918) 
               
               
                 UGCUCCCUCUCUCACAUCCCUUGCAUGGUGGAGGGUGAGCUUUCUGAAAACCCCUCCCACAUGCAGGGUUUGCAGGA 
               
               
                 UGGCGAGCC 
               
               
                   
               
               
                 &gt;hsa-mir-190a MI0000486 (SEQ ID NO: 919) 
               
               
                 UGCAGGCCUCUGUGUGAUAUGUUUGAUAUAUUAGGUUGUUAUUUAAUCCAACUAUAUAUCAAACAUAUUCCUACAGU 
               
               
                 GUCUUGCC 
               
               
                   
               
               
                 &gt;hsa-mir-190b MI0005545 (SEQ ID NO: 920) 
               
               
                 UGCUUCUGUGUGAUAUGUUUGAUAUUGGGUUGUUUAAUUAGGAACCAACUAAAUGUCAAACAUAUUCUUACAGCAGC 
               
               
                 AG 
               
               
                   
               
               
                 &gt;hsa-mir-191 MI0000465 (SEQ ID NO: 921) 
               
               
                 CGGCUGGACAGCGGGCAACGGAAUCCCAAAAGCAGCUGUUGUCUCCAGAGCAUUCCAGCUGCGCUUGGAUUUCGUCC 
               
               
                 CCUGCUCUCCUGCCU 
               
               
                   
               
               
                 &gt;hsa-mir-192 MI0000234 (SEQ ID NO: 922) 
               
               
                 GCCGAGACCGAGUGCACAGGGCUCUGACCUAUGAAUUGACAGCCAGUGCUCUCGUCUCCCCUCUGGCUGCCAAUUCC 
               
               
                 AUAGGUCACAGGUAUGUUCGCCUCAAUGCCAGC 
               
               
                   
               
               
                 &gt;hsa-mir-193a MI0000487 (SEQ ID NO: 923) 
               
               
                 CGAGGAUGGGAGCUGAGGGCUGGGUCUUUGCGGGCGAGAUGAGGGUGUCGGAUCAACUGGCCUACAAAGUCCCAGUU 
               
               
                 CUCGGCCCCCG 
               
               
                   
               
               
                 &gt;hsa-mir-193b MI0003137 (SEQ ID NO: 924) 
               
               
                 GUGGUCUCAGAAUCGGGGUUUUGAGGGCGAGAUGAGUUUAUGUUUUAUCCAACUGGCCCUCAAAGUCCCGCUUUUGG 
               
               
                 GGUCAU 
               
               
                   
               
               
                 &gt;hsa-mir-194-1 MI0000488 (SEQ ID NO: 925) 
               
               
                 AUGGUGUUAUCAAGUGUAACAGCAACUCCAUGUGGACUGUGUACCAAUUUCCAGUGGAGAUGCUGUUACUUUUGAUG 
               
               
                 GUUACCAA 
               
               
                   
               
               
                 &gt;hsa-mir-194-2 MI0000732 (SEQ ID NO: 926) 
               
               
                 UGGUUCCCGCCCCCUGUAACAGCAACUCCAUGUGGAAGUGCCCACUGGUUCCAGUGGGGCUGCUGUUAUCUGGGGCG 
               
               
                 AGGGCCAG 
               
               
                   
               
               
                 &gt;hsa-mir-195 MI0000489 (SEQ ID NO: 927) 
               
               
                 AGCUUCCCUGGCUCUAGCAGCACAGAAAUAUUGGCACAGGGAAGCGAGUCUGCCAAUAUUGGCUGUGCUGCUCCAGG 
               
               
                 CAGGGUGGUG 
               
               
                   
               
               
                 &gt;hsa-mir-196a-1 MI0000238 (SEQ ID NO: 928) 
               
               
                 GUGAAUUAGGUAGUUUCAUGUUGUUGGGCCUGGGUUUCUGAACACAACAACAUUAAACCACCCGAUUCAC 
               
               
                   
               
               
                 &gt;hsa-mir-196a-2 MI0000279 (SEQ ID NO: 929) 
               
               
                 UGCUCGCUCAGCUGAUCUGUGGCUUAGGUAGUUUCAUGUUGUUGGGAUUGAGUUUUGAACUCGGCAACAAGAAACUG 
               
               
                 CCUGAGUUACAUCAGUCGGUUUUCGUCGAGGGC 
               
               
                   
               
               
                 &gt;hsa-mir-196b MI0001150 (SEQ ID NO: 930) 
               
               
                 ACUGGUCGGUGAUUUAGGUAGUUUCCUGUUGUUGGGAUCCACCUUUCUCUCGACAGCACGACACUGCCUUCAUUACU 
               
               
                 UCAGUUG 
               
               
                   
               
               
                 &gt;hsa-mir-197 MI0000239 (SEQ ID NO: 931) 
               
               
                 GGCUGUGCCGGGUAGAGAGGGCAGUGGGAGGUAAGAGCUCUUCACCCUUCACCACCUUCUCCACCCAGCAUGGCC 
               
               
                   
               
               
                 &gt;hsa-mir-198 MI0000240 (SEQ ID NO: 932) 
               
               
                 UCAUUGGUCCAGAGGGGAGAUAGGUUCCUGUGAUUUUUCCUUCUUCUCUAUAGAAUAAAUGA 
               
               
                   
               
               
                 &gt;hsa-mir-199a-1 MI0000242 (SEQ ID NO: 933) 
               
               
                 GCCAACCCAGUGUUCAGACUACCUGUUCAGGAGGCUCUCAAUGUGUACAGUAGUCUGCACAUUGGUUAGGC 
               
               
                   
               
               
                 &gt;hsa-mir-199a-2 MI0000281 (SEQ ID NO: 934) 
               
               
                 AGGAAGCUUCUGGAGAUCCUGCUCCGUCGCCCCAGUGUUCAGACUACCUGUUCAGGACAAUGCCGUUGUACAGUAGU 
               
               
                 CUGCACAUUGGUUAGACUGGGCAAGGGAGAGCA 
               
               
                   
               
               
                 &gt;hsa-mir-199b MI0000282 (SEQ ID NO: 935) 
               
               
                 CCAGAGGACACCUCCACUCCGUCUACCCAGUGUUUAGACUAUCUGUUCAGGACUCCCAAAUUGUACAGUAGUCUGCA 
               
               
                 CAUUGGUUAGGCUGGGCUGGGUUAGACCCUCGG 
               
               
                   
               
               
                 &gt;hsa-mir-200a MI0000737 (SEQ ID NO: 936) 
               
               
                 CCGGGCCCCUGUGAGCAUCUUACCGGACAGUGCUGGAUUUCCCAGCUUGACUCUAACACUGUCUGGUAACGAUGUUC 
               
               
                 AAAGGUGACCCGC 
               
               
                   
               
               
                 &gt;hsa-mir-200b MI0000342 (SEQ ID NO: 937) 
               
               
                 CCAGCUCGGGCAGCCGUGGCCAUCUUACUGGGCAGCAUUGGAUGGAGUCAGGUCUCUAAUACUGCCUGGUAAUGAUG 
               
               
                 ACGGCGGAGCCCUGCACG 
               
               
                   
               
               
                 &gt;hsa-mir-200c MI0000650 (SEQ ID NO: 938) 
               
               
                 CCCUCGUCUUACCCAGCAGUGUUUGGGUGCGGUUGGGAGUCUCUAAUACUGCCGGGUAAUGAUGGAGG 
               
               
                   
               
               
                 &gt;hsa-mir-202 MI0003130 (SEQ ID NO: 939) 
               
               
                 CGCCUCAGAGCCGCCCGCCGUUCCUUUUUCCUAUGCAUAUACUUCUUUGAGGAUCUGGCCUAAAGAGGUAUAGGGCA 
               
               
                 UGGGAAAACGGGGCGGUCGGGUCCUCCCCAGCG 
               
               
                   
               
               
                 &gt;hsa-mir-203 MI0000283 (SEQ ID NO: 940) 
               
               
                 GUGUUGGGGACUCGCGCGCUGGGUCCAGUGGUUCUUAACAGUUCAACAGUUCUGUAGCGCAAUUGUGAAAUGUUUAG 
               
               
                 GACCACUAGACCCGGCGGGCGCGGCGACAGCGA 
               
               
                   
               
               
                 &gt;hsa-mir-204 MI0000284 (SEQ ID NO: 941) 
               
               
                 GGCUACAGUCUUUCUUCAUGUGACUCGUGGACUUCCCUUUGUCAUCCUAUGCCUGAGAAUAUAUGAAGGAGGCUGGG 
               
               
                 AAGGCAAAGGGACGUUCAAUUGUCAUCACUGGC 
               
               
                   
               
               
                 &gt;hsa-mir-205 MI0000285 (SEQ ID NO: 942) 
               
               
                 AAAGAUCCUCAGACAAUCCAUGUGCUUCUCUUGUCCUUCAUUCCACCGGAGUCUGUCUCAUACCCAACCAGAUUUCA 
               
               
                 GUGGAGUGAAGUUCAGGAGGCAUGGAGCUGACA 
               
               
                   
               
               
                 &gt;hsa-mir-206 MI0000490 (SEQ ID NO: 943) 
               
               
                 UGCUUCCCGAGGCCACAUGCUUCUUUAUAUCCCCAUAUGGAUUACUUUGCUAUGGAAUGUAAGGAAGUGUGUGGUUU 
               
               
                 CGGCAAGUG 
               
               
                   
               
               
                 &gt;hsa-mir-208a MI0000251 (SEQ ID NO: 944) 
               
               
                 UGACGGGCGAGCUUUUGGCCCGGGUUAUACCUGAUGCUCACGUAUAAGACGAGCAAAAAGCUUGUUGGUCA 
               
               
                   
               
               
                 &gt;hsa-mir-208b MI0005570 (SEQ ID NO: 945) 
               
               
                 CCUCUCAGGGAAGCUUUUUGCUCGAAUUAUGUUUCUGAUCCGAAUAUAAGACGAACAAAAGGUUUGUCUGAGGGCAG 
               
               
                   
               
               
                 &gt;hsa-mir-210 MI0000286 (SEQ ID NO: 946) 
               
               
                 ACCCGGCAGUGCCUCCAGGCGCAGGGCAGCCCCUGCCCACCGCACACUGCGCUGCCCCAGACCCACUGUGCGUGUGA 
               
               
                 CAGCGGCUGAUCUGUGCCUGGGCAGCGCGACCC 
               
               
                   
               
               
                 &gt;hsa-mir-211 MI0000287 (SEQ ID NO: 947) 
               
               
                 UCACCUGGCCAUGUGACUUGUGGGCUUCCCUUUGUCAUCCUUCGCCUAGGGCUCUGAGCAGGGCAGGGACAGCAAAG 
               
               
                 GGGUGCUCAGUUGUCACUUCCCACAGCACGGAG 
               
               
                   
               
               
                 &gt;hsa-mir-212 MI0000288 (SEQ ID NO: 948) 
               
               
                 CGGGGCACCCCGCCCGGACAGCGCGCCGGCACCUUGGCUCUAGACUGCUUACUGCCCGGGCCGCCCUCAGUAACAGU 
               
               
                 CUCCAGUCACGGCCACCGACGCCUGGCCCCGCC 
               
               
                   
               
               
                 &gt;hsa-mir-214 MI0000290 (SEQ ID NO: 949) 
               
               
                 GGCCUGGCUGGACAGAGUUGUCAUGUGUCUGCCUGUCUACACUUGCUGUGCAGAACAUCCGCUCACCUGUACAGCAG 
               
               
                 GCACAGACAGGCAGUCACAUGACAACCCAGCCU 
               
               
                   
               
               
                 &gt;hsa-mir-215 MI0000291 (SEQ ID NO: 950) 
               
               
                 AUCAUUCAGAAAUGGUAUACAGGAAAAUGACCUAUGAAUUGACAGACAAUAUAGCUGAGUUUGUCUGUCAUUUCUUU 
               
               
                 AGGCCAAUAUUCUGUAUGACUGUGCUACUUCAA 
               
               
                   
               
               
                 &gt;hsa-mir-216a MI0000292 (SEQ ID NO: 951) 
               
               
                 GAUGGCUGUGAGUUGGCUUAAUCUCAGCUGGCAACUGUGAGAUGUUCAUACAAUCCCUCACAGUGGUCUCUGGGAUU 
               
               
                 AUGCUAAACAGAGCAAUUUCCUAGCCCUCACGA 
               
               
                   
               
               
                 &gt;hsa-mir-216b MI0005569 (SEQ ID NO: 952) 
               
               
                 GCAGACUGGAAAAUCUCUGCAGGCAAAUGUGAUGUCACUGAGGAAAUCACACACUUACCCGUAGAGAUUCUACAGUC 
               
               
                 UGACA 
               
               
                   
               
               
                 &gt;hsa-mir-217 MI0000293 (SEQ ID NO: 953) 
               
               
                 AGUAUAAUUAUUACAUAGUUUUUGAUGUCGCAGAUACUGCAUCAGGAACUGAUUGGAUAAGAAUCAGUCACCAUCAG 
               
               
                 UUCCUAAUGCAUUGCCUUCAGCAUCUAAACAAG 
               
               
                   
               
               
                 &gt;hsa-mir-218-1 MI0000294 (SEQ ID NO: 954) 
               
               
                 GUGAUAAUGUAGCGAGAUUUUCUGUUGUGCUUGAUCUAACCAUGUGGUUGCGAGGUAUGAGUAAAACAUGGUUCCGU 
               
               
                 CAAGCACCAUGGAACGUCACGCAGCUUUCUACA 
               
               
                   
               
               
                 &gt;hsa-mir-218-2 MI0000295 (SEQ ID NO: 955) 
               
               
                 GACCAGUCGCUGCGGGGCUUUCCUUUGUGCUUGAUCUAACCAUGUGGUGGAACGAUGGAAACGGAACAUGGUUCUGU 
               
               
                 CAAGCACCGCGGAAAGCACCGUGCUCUCCUGCA 
               
               
                   
               
               
                 &gt;hsa-mir-219-1 MI0000296 (SEQ ID NO: 956) 
               
               
                 CCGCCCCGGGCCGCGGCUCCUGAUUGUCCAAACGCAAUUCUCGAGUCUAUGGCUCCGGCCGAGAGUUGAGUCUGGAC 
               
               
                 GUCCCGAGCCGCCGCCCCCAAACCUCGAGCGGG 
               
               
                   
               
               
                 &gt;hsa-mir-219-2 MI0000740 (SEQ ID NO: 957) 
               
               
                 ACUCAGGGGCUUCGCCACUGAUUGUCCAAACGCAAUUCUUGUACGAGUCUGCGGCCAACCGAGAAUUGUGGCUGGAC 
               
               
                 AUCUGUGGCUGAGCUCCGGG 
               
               
                   
               
               
                 &gt;hsa-mir-221 MI0000298 (SEQ ID NO: 958) 
               
               
                 UGAACAUCCAGGUCUGGGGCAUGAACCUGGCAUACAAUGUAGAUUUCUGUGUUCGUUAGGCAACAGCUACAUUGUCU 
               
               
                 GCUGGGUUUCAGGCUACCUGGAAACAUGUUCUC 
               
               
                   
               
               
                 &gt;hsa-mir-222 MI0000299 (SEQ ID NO: 959) 
               
               
                 GCUGCUGGAAGGUGUAGGUACCCUCAAUGGCUCAGUAGCCAGUGUAGAUCCUGUCUUUCGUAAUCAGCAGCUACAUC 
               
               
                 UGGCUACUGGGUCUCUGAUGGCAUCUUCUAGCU 
               
               
                   
               
               
                 &gt;hsa-mir-223 MI0000300 (SEQ ID NO: 960) 
               
               
                 CCUGGCCUCCUGCAGUGCCACGCUCCGUGUAUUUGACAAGCUGAGUUGGACACUCCAUGUGGUAGAGUGUCAGUUUG 
               
               
                 UCAAAUACCCCAAGUGCGGCACAUGCUUACCAG 
               
               
                   
               
               
                 &gt;hsa-mir-224 MI0000301 (SEQ ID NO: 961) 
               
               
                 GGGCUUUCAAGUCACUAGUGGUUCCGUUUAGUAGAUGAUUGUGCAUUGUUUCAAAAUGGUGCCCUAGUGACUACAAA 
               
               
                 GCCC 
               
               
                   
               
               
                 &gt;hsa-mir-296 MI0000747 (SEQ ID NO: 962) 
               
               
                 AGGACCCUUCCAGAGGGCCCCCCCUCAAUCCUGUUGUGCCUAAUUCAGAGGGUUGGGUGGAGGCUCUCCUGAAGGGC 
               
               
                 UCU 
               
               
                   
               
               
                 &gt;hsa-mir-297 MI0005775 (SEQ ID NO: 963) 
               
               
                 UGUAUGUAUGUGUGCAUGUGCAUGUAUGUGUAUAUACAUAUAUAUGUAUUAUGUACUCAUAUAUCA 
               
               
                   
               
               
                 &gt;hsa-mir-298 MI0005523 (SEQ ID NO: 964) 
               
               
                 UCAGGUCUUCAGCAGAAGCAGGGAGGUUCUCCCAGUGGUUUUCCUUGACUGUGAGGAACUAGCCUGCUGCUUUGCUC 
               
               
                 AGGAGUGAGCU 
               
               
                   
               
               
                 &gt;hsa-mir-299 MI0000744 (SEQ ID NO: 965) 
               
               
                 AAGAAAUGGUUUACCGUCCCACAUACAUUUUGAAUAUGUAUGUGGGAUGGUAAACCGCUUCUU 
               
               
                   
               
               
                 &gt;hsa-mir-300 MI0005525 (SEQ ID NO: 966) 
               
               
                 UGCUACUUGAAGAGAGGUAAUCCUUCACGCAUUUGCUUUACUUGCAAUGAUUAUACAAGGGCAGACUCUCUCUGGGG 
               
               
                 AGCAAA 
               
               
                   
               
               
                 &gt;hsa-mir-301a MI0000745 (SEQ ID NO: 967) 
               
               
                 ACUGCUAACGAAUGCUCUGACUUUAUUGCACUACUGUACUUUACAGCUAGCAGUGCAAUAGUAUUGUCAAAGCAUCU 
               
               
                 GAAAGCAGG 
               
               
                   
               
               
                 &gt;hsa-mir-301b MI0005568 (SEQ ID NO: 968) 
               
               
                 GCCGCAGGUGCUCUGACGAGGUUGCACUACUGUGCUCUGAGAAGCAGUGCAAUGAUAUUGUCAAAGCAUCUGGGACCA 
               
               
                   
               
               
                 &gt;hsa-mir-302a MI0000738 (SEQ ID NO: 969) 
               
               
                 CCACCACUUAAACGUGGAUGUACUUGCUUUGAAACUAAAGAAGUAAGUGCUUCCAUGUUUUGGUGAUGG 
               
               
                   
               
               
                 &gt;hsa-mir-302b MI0000772 (SEQ ID NO: 970) 
               
               
                 GCUCCCUUCAACUUUAACAUGGAAGUGCUUUCUGUGACUUUAAAAGUAAGUGCUUCCAUGUUUUAGUAGGAGU 
               
               
                   
               
               
                 &gt;hsa-mir-302c MI0000773 (SEQ ID NO: 971) 
               
               
                 CCUUUGCUUUAACAUGGGGGUACCUGCUGUGUGAAACAAAAGUAAGUGCUUCCAUGUUUCAGUGGAGG 
               
               
                   
               
               
                 &gt;hsa-mir-302d MI0000774 (SEQ ID NO: 972) 
               
               
                 CCUCUACUUUAACAUGGAGGCACUUGCUGUGACAUGACAAAAAUAAGUGCUUCCAUGUUUGAGUGUGG 
               
               
                   
               
               
                 &gt;hsa-mir-302e MI0006417 (SEQ ID NO: 973) 
               
               
                 UUGGGUAAGUGCUUCCAUGCUUCAGUUUCCUUACUGGUAAGAUGGAUGUAGUAAUAGCACCUACCUUAUAGA 
               
               
                   
               
               
                 &gt;hsa-mir-302f MI0006418 (SEQ ID NO: 974) 
               
               
                 UCUGUGUAAACCUGGCAAUUUUCACUUAAUUGCUUCCAUGUUUAUAAAAGA 
               
               
                   
               
               
                 &gt;hsa-mir-320a MI0000542 (SEQ ID NO: 975) 
               
               
                 GCUUCGCUCCCCUCCGCCUUCUCUUCCCGGUUCUUCCCGGAGUCGGGAAAAGCUGGGUUGAGAGGGCGAAAAAGGAU 
               
               
                 GAGGU 
               
               
                   
               
               
                 &gt;hsa-mir-320b-1 MI0003776 (SEQ ID NO: 976) 
               
               
                 AAUUAAUCCCUCUCUUUCUAGUUCUUCCUAGAGUGAGGAAAAGCUGGGUUGAGAGGGCAAACAAAUUAACUAAUUAA 
               
               
                 UU 
               
               
                   
               
               
                 &gt;hsa-mir-320b-2 MI0003839 (SEQ ID NO: 977) 
               
               
                 UGUUAUUUUUUGUCUUCUACCUAAGAAUUCUGUCUCUUAGGCUUUCUCUUCCCAGAUUUCCCAAAGUUGGGAAAAGC 
               
               
                 UGGGUUGAGAGGGCAAAAGGAAAAAAAAAGAAUUCUGUCUCUGACAUAAUUAGAUAGGGAA 
               
               
                   
               
               
                 &gt;hsa-mir-320c-1 MI0003778 (SEQ ID NO: 978) 
               
               
                 UUUGCAUUAAAAAUGAGGCCUUCUCUUCCCAGUUCUUCCCAGAGUCAGGAAAAGCUGGGUUGAGAGGGUAGAAAAAA 
               
               
                 AAUGAUGUAGG 
               
               
                   
               
               
                 &gt;hsa-mir-320c-2 MI0008191 (SEQ ID NO: 979) 
               
               
                 CUUCUCUUUCCAGUUCUUCCCAGAAUUGGGAAAAGCUGGGUUGAGAGGGU 
               
               
                   
               
               
                 &gt;hsa-mir-320d-1 MI0008190 (SEQ ID NO: 980) 
               
               
                 UUCUCGUCCCAGUUCUUCCCAAAGUUGAGAAAAGCUGGGUUGAGAGGA 
               
               
                   
               
               
                 &gt;hsa-mir-320d-2 MI0008192 (SEQ ID NO: 981) 
               
               
                 UUCUCUUCCCAGUUCUUCUUGGAGUCAGGAAAAGCUGGGUUGAGAGGA 
               
               
                   
               
               
                 &gt;hsa-mir-320e MI0014234 (SEQ ID NO: 982) 
               
               
                 GCCUUCUCUUCCCAGUUCUUCCUGGAGUCGGGGAAAAGCUGGGUUGAGAAGGU 
               
               
                   
               
               
                 &gt;hsa-mir-323a MI0000807 (SEQ ID NO: 983) 
               
               
                 UUGGUACUUGGAGAGAGGUGGUCCGUGGCGCGUUCGCUUUAUUUAUGGCGCACAUUACACGGUCGACCUCUUUGCAG 
               
               
                 UAUCUAAUC 
               
               
                   
               
               
                 &gt;hsa-mir-323b MI0014206 (SEQ ID NO: 984) 
               
               
                 UGGUACUCGGAGGGAGGUUGUCCGUGGUGAGUUCGCAUUAUUUAAUGAUGCCCAAUACACGGUCGACCUCUUUUCGG 
               
               
                 UAUCA 
               
               
                   
               
               
                 &gt;hsa-mir-324 MI0000813 (SEQ ID NO: 985) 
               
               
                 CUGACUAUGCCUCCCCGCAUCCCCUAGGGCAUUGGUGUAAAGCUGGAGACCCACUGCCCCAGGUGCUGCUGGGGGUU 
               
               
                 GUAGUC 
               
               
                   
               
               
                 &gt;hsa-mir-325 MI0000824 (SEQ ID NO: 986) 
               
               
                 AUACAGUGCUUGGUUCCUAGUAGGUGUCCAGUAAGUGUUUGUGACAUAAUUUGUUUAUUGAGGACCUCCUAUCAAUC 
               
               
                 AAGCACUGUGCUAGGCUCUGG 
               
               
                   
               
               
                 &gt;hsa-mir-326 MI0000808 (SEQ ID NO: 987) 
               
               
                 CUCAUCUGUCUGUUGGGCUGGAGGCAGGGCCUUUGUGAAGGCGGGUGGUGCUCAGAUCGCCUCUGGGCCCUUCCUCC 
               
               
                 AGCCCCGAGGCGGAUUCA 
               
               
                   
               
               
                 &gt;hsa-mir-328 MI0000804 (SEQ ID NO: 988) 
               
               
                 UGGAGUGGGGGGGCAGGAGGGGCUCAGGGAGAAAGUGCAUACAGCCCCUGGCCCUCUCUGCCCUUCCGUCCCCUG 
               
               
                   
               
               
                 &gt;hsa-mir-329-1 MI0001725 (SEQ ID NO: 989) 
               
               
                 GGUACCUGAAGAGAGGUUUUCUGGGUUUCUGUUUCUUUAAUGAGGACGAAACACACCUGGUUAACCUCUUUUCCAGU 
               
               
                 AUC 
               
               
                   
               
               
                 &gt;hsa-mir-329-2 MI0001726 (SEQ ID NO: 990) 
               
               
                 GUGGUACCUGAAGAGAGGUUUUCUGGGUUUCUGUUUCUUUAUUGAGGACGAAACACACCUGGUUAACCUCUUUUCCA 
               
               
                 GUAUCAA 
               
               
                   
               
               
                 &gt;hsa-mir-330 MI0000803 (SEQ ID NO: 991) 
               
               
                 CUUUGGCGAUCACUGCCUCUCUGGGCCUGUGUCUUAGGCUCUGCAAGAUCAACCGAGCAAAGCACACGGCCUGCAGA 
               
               
                 GAGGCAGCGCUCUGCCC 
               
               
                   
               
               
                 &gt;hsa-mir-331 MI0000812 (SEQ ID NO: 992) 
               
               
                 GAGUUUGGUUUUGUUUGGGUUUGUUCUAGGUAUGGUCCCAGGGAUCCCAGAUCAAACCAGGCCCCUGGGCCUAUCCU 
               
               
                 AGAACCAACCUAAGCUC 
               
               
                   
               
               
                 &gt;hsa-mir-335 MI0000816 (SEQ ID NO: 993) 
               
               
                 UGUUUUGAGCGGGGGUCAAGAGCAAUAACGAAAAAUGUUUGUCAUAAACCGUUUUUCAUUAUUGCUCCUGACCUCCU 
               
               
                 CUCAUUUGCUAUAUUCA 
               
               
                   
               
               
                 &gt;hsa-mir-337 MI0000806 (SEQ ID NO: 994) 
               
               
                 GUAGUCAGUAGUUGGGGGGUGGGAACGGCUUCAUACAGGAGUUGAUGCACAGUUAUCCAGCUCCUAUAUGAUGCCUU 
               
               
                 UCUUCAUCCCCUUCAA 
               
               
                   
               
               
                 &gt;hsa-mir-338 MI0000814 (SEQ ID NO: 995) 
               
               
                 UCUCCAACAAUAUCCUGGUGCUGAGUGAUGACUCAGGCGACUCCAGCAUCAGUGAUUUUGUUGAAGA 
               
               
                   
               
               
                 &gt;hsa-mir-339 MI0000815 (SEQ ID NO: 996) 
               
               
                 CGGGGCGGCCGCUCUCCCUGUCCUCCAGGAGCUCACGUGUGCCUGCCUGUGAGCGCCUCGACGACAGAGCCGGCGCC 
               
               
                 UGCCCCAGUGUCUGCGC 
               
               
                   
               
               
                 &gt;hsa-mir-340 MI0000802 (SEQ ID NO: 997) 
               
               
                 UUGUACCUGGUGUGAUUAUAAAGCAAUGAGACUGAUUGUCAUAUGUCGUUUGUGGGAUCCGUCUCAGUUACUUUAUA 
               
               
                 GCCAUACCUGGUAUCUUA 
               
               
                   
               
               
                 &gt;hsa-mir-342 MI0000805 (SEQ ID NO: 998) 
               
               
                 GAAACUGGGCUCAAGGUGAGGGGUGCUAUCUGUGAUUGAGGGACAUGGUUAAUGGAAUUGUCUCACACAGAAAUCGC 
               
               
                 ACCCGUCACCUUGGCCUACUUA 
               
               
                   
               
               
                 &gt;hsa-mir-345 MI0000825 (SEQ ID NO: 999) 
               
               
                 ACCCAAACCCUAGGUCUGCUGACUCCUAGUCCAGGGCUCGUGAUGGCUGGUGGGCCCUGAACGAGGGGUCUGGAGGC 
               
               
                 CUGGGUUUGAAUAUCGACAGC 
               
               
                   
               
               
                 &gt;hsa-mir-346 MI0000826 (SEQ ID NO: 1000) 
               
               
                 GGUCUCUGUGUUGGGCGUCUGUCUGCCCGCAUGCCUGCCUCUCUGUUGCUCUGAAGGAGGCAGGGGCUGGGCCUGCA 
               
               
                 GCUGCCUGGGCAGAGCGG 
               
               
                   
               
               
                 &gt;hsa-mir-361 MI0000760 (SEQ ID NO: 1001) 
               
               
                 GGAGCUUAUCAGAAUCUCCAGGGGUACUUUAUAAUUUCAAAAAGUCCCCCAGGUGUGAUUCUGAUUUGCUUC 
               
               
                   
               
               
                 &gt;hsa-mir-362 MI0000762 (SEQ ID NO: 1002) 
               
               
                 CUUGAAUCCUUGGAACCUAGGUGUGAGUGCUAUUUCAGUGCAACACACCUAUUCAAGGAUUCAAA 
               
               
                   
               
               
                 &gt;hsa-mir-363 MI0000764 (SEQ ID NO: 1003) 
               
               
                 UGUUGUCGGGUGGAUCACGAUGCAAUUUUGAUGAGUAUCAUAGGAGAAAAAUUGCACGGUAUCCAUCUGUAAACC 
               
               
                   
               
               
                 &gt;hsa-mir-365a MI0000767 (SEQ ID NO: 1004) 
               
               
                 ACCGCAGGGAAAAUGAGGGACUUUUGGGGGCAGAUGUGUUUCCAUUCCACUAUCAUAAUGCCCCUAAAAAUCCUUAU 
               
               
                 UGCUCUUGCA 
               
               
                   
               
               
                 &gt;hsa-mir-365b MI0000769 (SEQ ID NO: 1005) 
               
               
                 AGAGUGUUCAAGGACAGCAAGAAAAAUGAGGGACUUUCAGGGGCAGCUGUGUUUUCUGACUCAGUCAUAAUGCCCCU 
               
               
                 AAAAAUCCUUAUUGUUCUUGCAGUGUGCAUCGGG 
               
               
                   
               
               
                 &gt;hsa-mir-367 MI0000775 (SEQ ID NO: 1006) 
               
               
                 CCAUUACUGUUGCUAAUAUGCAACUCUGUUGAAUAUAAAUUGGAAUUGCACUUUAGCAAUGGUGAUGG 
               
               
                   
               
               
                 &gt;hsa-mir-369 MI0000777 (SEQ ID NO: 1007) 
               
               
                 UUGAAGGGAGAUCGACCGUGUUAUAUUCGCUUUAUUGACUUCGAAUAAUACAUGGUUGAUCUUUUCUCAG 
               
               
                   
               
               
                 &gt;hsa-mir-370 MI0000778 (SEQ ID NO: 1008) 
               
               
                 AGACAGAGAAGCCAGGUCACGUCUCUGCAGUUACACAGCUCACGAGUGCCUGCUGGGGUGGAACCUGGUCUGUCU 
               
               
                   
               
               
                 &gt;hsa-mir-371a MI0000779 (SEQ ID NO: 1009) 
               
               
                 GUGGCACUCAAACUGUGGGGGCACUUUCUGCUCUCUGGUGAAAGUGCCGCCAUCUUUUGAGUGUUAC 
               
               
                   
               
               
                 &gt;hsa-mir-371b MI0017393 (SEQ ID NO: 1010) 
               
               
                 GGUAACACUCAAAAGAUGGCGGCACUUUCACCAGAGAGCAGAAAGUGCCCCCACAGUUUGAGUGCC 
               
               
                   
               
               
                 &gt;hsa-mir-372 MI0000780 (SEQ ID NO: 1011) 
               
               
                 GUGGGCCUCAAAUGUGGAGCACUAUUCUGAUGUCCAAGUGGAAAGUGCUGCGACAUUUGAGCGUCAC 
               
               
                   
               
               
                 &gt;hsa-mir-373 MI0000781 (SEQ ID NO: 1012) 
               
               
                 GGGAUACUCAAAAUGGGGGCGCUUUCCUUUUUGUCUGUACUGGGAAGUGCUUCGAUUUUGGGGUGUCCC 
               
               
                   
               
               
                 &gt;hsa-mir-374a MI0000782 (SEQ ID NO: 1013) 
               
               
                 UACAUCGGCCAUUAUAAUACAACCUGAUAAGUGUUAUAGCACUUAUCAGAUUGUAUUGUAAUUGUCUGUGUA 
               
               
                   
               
               
                 &gt;hsa-mir-374b MI0005566 (SEQ ID NO: 1014) 
               
               
                 ACUCGGAUGGAUAUAAUACAACCUGCUAAGUGUCCUAGCACUUAGCAGGUUGUAUUAUCAUUGUCCGUGUCU 
               
               
                   
               
               
                 &gt;hsa-mir-374c MI0016684 (SEQ ID NO: 1015) 
               
               
                 ACACGGACAAUGAUAAUACAACCUGCUAAGUGCUAGGACACUUAGCAGGUUGUAUUAUAUCCAUCCGAGU 
               
               
                   
               
               
                 &gt;hsa-mir-375 MI0000783 (SEQ ID NO: 1016) 
               
               
                 CCCCGCGACGAGCCCCUCGCACAAACCGGACCUGAGCGUUUUGUUCGUUCGGCUCGCGUGAGGC 
               
               
                   
               
               
                 &gt;hsa-mir-376a-1 MI0000784 (SEQ ID NO: 1017) 
               
               
                 UAAAAGGUAGAUUCUCCUUCUAUGAGUACAUUAUUUAUGAUUAAUCAUAGAGGAAAAUCCACGUUUUC 
               
               
                   
               
               
                 &gt;hsa-mir-376a-2 MI0003529 (SEQ ID NO: 1018) 
               
               
                 GGUAUUUAAAAGGUAGAUUUUCCUUCUAUGGUUACGUGUUUGAUGGUUAAUCAUAGAGGAAAAUCCACGUUUUCAGU 
               
               
                 AUC 
               
               
                   
               
               
                 &gt;hsa-mir-376b MI0002466 (SEQ ID NO: 1019) 
               
               
                 CAGUCCUUCUUUGGUAUUUAAAACGUGGAUAUUCCUUCUAUGUUUACGUGAUUCCUGGUUAAUCAUAGAGGAAAAUC 
               
               
                 CAUGUUUUCAGUAUCAAAUGCUG 
               
               
                   
               
               
                 &gt;hsa-mir-376c MI0000776 (SEQ ID NO: 1020) 
               
               
                 AAAAGGUGGAUAUUCCUUCUAUGUUUAUGUUAUUUAUGGUUAAACAUAGAGGAAAUUCCACGUUUU 
               
               
                   
               
               
                 &gt;hsa-mir-377 MI0000785 (SEQ ID NO: 1021) 
               
               
                 UUGAGCAGAGGUUGCCCUUGGUGAAUUCGCUUUAUUUAUGUUGAAUCACACAAAGGCAACUUUUGUUUG 
               
               
                   
               
               
                 &gt;hsa-mir-378a MI0000786 (SEQ ID NO: 1022) 
               
               
                 AGGGCUCCUGACUCCAGGUCCUGUGUGUUACCUAGAAAUAGCACUGGACUUGGAGUCAGAAGGCCU 
               
               
                   
               
               
                 &gt;hsa-mir-378b MI0014154 (SEQ ID NO: 1023) 
               
               
                 GGUCAUUGAGUCUUCAAGGCUAGUGGAAAGAGCACUGGACUUGGAGGCAGAAAGACC 
               
               
                   
               
               
                 &gt;hsa-mir-378c MI0015825 (SEQ ID NO: 1024) 
               
               
                 GGAGGCCAUCACUGGACUUGGAGUCAGAAGAGUGGAGUCGGGUCAGACUUCAACUCUGACUUUGAAGGUGGUGAGUG 
               
               
                 CCUC 
               
               
                   
               
               
                 &gt;hsa-mir-378d-1 MI0016749 (SEQ ID NO: 1025) 
               
               
                 ACUGUUUCUGUCCUUGUUCUUGUUGUUAUUACUGGACUUGGAGUCAGAAACAGG 
               
               
                   
               
               
                 &gt;hsa-mir-378d-2 MI0003840 (SEQ ID NO: 1026) 
               
               
                 GAAUGGUUACAAGGAGAGAACACUGGACUUGGAGUCAGAAAACUUUCAUCCAAGUCAUUCCCUGCUCUAAGUCCCAU 
               
               
                 UUCUGUUCCAUGAGAUUGUUU 
               
               
                   
               
               
                 &gt;hsa-mir-378e MI0016750 (SEQ ID NO: 1027) 
               
               
                 CUGACUCCAGUGUCCAGGCCAGGGGCAGACAGUGGACAGAGAACAGUGCCCAAGACCACUGGACUUGGAGUCAGGAC 
               
               
                 AU 
               
               
                   
               
               
                 &gt;hsa-mir-378f MI0016756 (SEQ ID NO: 1028) 
               
               
                 GUCAGGUCCUGGACUCCCAUAGUUUUCAGGCUGCUAAACAACAGAACGAGCACUGGACUUGGAGCCAGAAGUCUUGGG 
               
               
                   
               
               
                 &gt;hsa-mir-378g MI0016761 (SEQ ID NO: 1029) 
               
               
                 CACUGGGCUUGGAGUCAGAAGACCUGGCUCCAGCCCAGCUC 
               
               
                   
               
               
                 &gt;hsa-mir-378h MI0016808 (SEQ ID NO: 1030) 
               
               
                 ACAGGAACACUGGACUUGGUGUCAGAUGGGAUGAGCCCUGGCUCUGUUUCCUAGCAGCAAUCUGAUCUUGAGCUAGU 
               
               
                 CACUGG 
               
               
                   
               
               
                 &gt;hsa-mir-378i MI0016902 (SEQ ID NO: 1031) 
               
               
                 GGGAGCACUGGACUAGGAGUCAGAAGGUGGAGUUCUGGGUGCUGUUUUCCCACUCUUGGGCCCUGGGCAUGUUCUG 
               
               
                   
               
               
                 &gt;hsa-mir-379 MI0000787 (SEQ ID NO: 1032) 
               
               
                 AGAGAUGGUAGACUAUGGAACGUAGGCGUUAUGAUUUCUGACCUAUGUAACAUGGUCCACUAACUCU 
               
               
                   
               
               
                 &gt;hsa-mir-380 MI0000788 (SEQ ID NO: 1033) 
               
               
                 AAGAUGGUUGACCAUAGAACAUGCGCUAUCUCUGUGUCGUAUGUAAUAUGGUCCACAUCUU 
               
               
                   
               
               
                 &gt;hsa-mir-381 MI0000789 (SEQ ID NO: 1034) 
               
               
                 UACUUAAAGCGAGGUUGCCCUUUGUAUAUUCGGUUUAUUGACAUGGAAUAUACAAGGGCAAGCUCUCUGUGAGUA 
               
               
                   
               
               
                 &gt;hsa-mir-382 MI0000790 (SEQ ID NO: 1035) 
               
               
                 UACUUGAAGAGAAGUUGUUCGUGGUGGAUUCGCUUUACUUAUGACGAAUCAUUCACGGACAACACUUUUUUCAGUA 
               
               
                   
               
               
                 &gt;hsa-mir-383 MI0000791 (SEQ ID NO: 1036) 
               
               
                 CUCCUCAGAUCAGAAGGUGAUUGUGGCUUUGGGUGGAUAUUAAUCAGCCACAGCACUGCCUGGUCAGAAAGAG 
               
               
                   
               
               
                 &gt;hsa-mir-384 MI0001145 (SEQ ID NO: 1037) 
               
               
                 UGUUAAAUCAGGAAUUUUAAACAAUUCCUAGACAAUAUGUAUAAUGUUCAUAAGUCAUUCCUAGAAAUUGUUCAUAA 
               
               
                 UGCCUGUAACA 
               
               
                   
               
               
                 &gt;hsa-mir-409 MI0001735 (SEQ ID NO: 1038) 
               
               
                 UGGUACUCGGGGAGAGGUUACCCGAGCAACUUUGCAUCUGGACGACGAAUGUUGCUCGGUGAACCCCUUUUCGGUAU 
               
               
                 CA 
               
               
                   
               
               
                 &gt;hsa-mir-410 MI0002465 (SEQ ID NO: 1039) 
               
               
                 GGUACCUGAGAAGAGGUUGUCUGUGAUGAGUUCGCUUUUAUUAAUGACGAAUAUAACACAGAUGGCCUGUUUUCAGU 
               
               
                 ACC 
               
               
                   
               
               
                 &gt;hsa-mir-411 MI0003675 (SEQ ID NO: 1040) 
               
               
                 UGGUACUUGGAGAGAUAGUAGACCGUAUAGCGUACGCUUUAUCUGUGACGUAUGUAACACGGUCCACUAACCCUCAG 
               
               
                 UAUCAAAUCCAUCCCCGAG 
               
               
                   
               
               
                 &gt;hsa-mir-412 MI0002464 (SEQ ID NO: 1041) 
               
               
                 CUGGGGUACGGGGAUGGAUGGUCGACCAGUUGGAAAGUAAUUGUUUCUAAUGUACUUCACCUGGUCCACUAGCCGUC 
               
               
                 CGUAUCCGCUGCAG 
               
               
                   
               
               
                 &gt;hsa-mir-421 MI0003685 (SEQ ID NO: 1042) 
               
               
                 GCACAUUGUAGGCCUCAUUAAAUGUUUGUUGAAUGAAAAAAUGAAUCAUCAACAGACAUUAAUUGGGCGCCUGCUCU 
               
               
                 GUGAUCUC 
               
               
                   
               
               
                 &gt;hsa-mir-422a MI0001444 (SEQ ID NO: 1043) 
               
               
                 GAGAGAAGCACUGGACUUAGGGUCAGAAGGCCUGAGUCUCUCUGCUGCAGAUGGGCUCUCUGUCCCUGAGCCAAGCU 
               
               
                 UUGUCCUCCCUGG 
               
               
                   
               
               
                 &gt;hsa-mir-423 MI0001445 (SEQ ID NO: 1044) 
               
               
                 AUAAAGGAAGUUAGGCUGAGGGGCAGAGAGCGAGACUUUUCUAUUUUCCAAAAGCUCGGUCUGAGGCCCCUCAGUCU 
               
               
                 UGCUUCCUAACCCGCGC 
               
               
                   
               
               
                 &gt;hsa-mir-424 MI0001446 (SEQ ID NO: 1045) 
               
               
                 CGAGGGGAUACAGCAGCAAUUCAUGUUUUGAAGUGUUCUAAAUGGUUCAAAACGUGAGGCGCUGCUAUACCCCCUCG 
               
               
                 UGGGGAAGGUAGAAGGUGGGG 
               
               
                   
               
               
                 &gt;hsa-mir-425 MI0001448 (SEQ ID NO: 1046) 
               
               
                 GAAAGCGCUUUGGAAUGACACGAUCACUCCCGUUGAGUGGGCACCCGAGAAGCCAUCGGGAAUGUCGUGUCCGCCCA 
               
               
                 GUGCUCUUUC 
               
               
                   
               
               
                 &gt;hsa-mir-429 MI0001641 (SEQ ID NO: 1047) 
               
               
                 CGCCGGCCGAUGGGCGUCUUACCAGACAUGGUUAGACCUGGCCCUCUGUCUAAUACUGUCUGGUAAAACCGUCCAUC 
               
               
                 CGCUGC 
               
               
                   
               
               
                 &gt;hsa-mir-431 MI0001721 (SEQ ID NO: 1048) 
               
               
                 UCCUGCUUGUCCUGCGAGGUGUCUUGCAGGCCGUCAUGCAGGCCACACUGACGGUAACGUUGCAGGUCGUCUUGCAG 
               
               
                 GGCUUCUCGCAAGACGACAUCCUCAUCACCAACGACG 
               
               
                   
               
               
                 &gt;hsa-mir-432 MI0003133 (SEQ ID NO: 1049) 
               
               
                 UGACUCCUCCAGGUCUUGGAGUAGGUCAUUGGGUGGAUCCUCUAUUUCCUUACGUGGGCCACUGGAUGGCUCCUCCA 
               
               
                 UGUCUUGGAGUAGAUCA 
               
               
                   
               
               
                 &gt;hsa-mir-433 MI0001723 (SEQ ID NO: 1050) 
               
               
                 CCGGGGAGAAGUACGGUGAGCCUGUCAUUAUUCAGAGAGGCUAGAUCCUCUGUGUUGAGAAGGAUCAUGAUGGGCUC 
               
               
                 CUCGGUGUUCUCCAGG 
               
               
                   
               
               
                 &gt;hsa-mir-448 MI0001637 (SEQ ID NO: 1051) 
               
               
                 GCCGGGAGGUUGAACAUCCUGCAUAGUGCUGCCAGGAAAUCCCUAUUUCAUAUAAGAGGGGGCUGGCUGGUUGCAUA 
               
               
                 UGUAGGAUGUCCCAUCUCCCAGCCCACUUCGUCA 
               
               
                   
               
               
                 &gt;hsa-mir-449a MI0001648 (SEQ ID NO: 1052) 
               
               
                 CUGUGUGUGAUGAGCUGGCAGUGUAUUGUUAGCUGGUUGAAUAUGUGAAUGGCAUCGGCUAACAUGCAACUGCUGUC 
               
               
                 UUAUUGCAUAUACA 
               
               
                   
               
               
                 &gt;hsa-mir-449b MI0003673 (SEQ ID NO: 1053) 
               
               
                 UGACCUGAAUCAGGUAGGCAGUGUAUUGUUAGCUGGCUGCUUGGGUCAAGUCAGCAGCCACAACUACCCUGCCACUU 
               
               
                 GCUUCUGGAUAAAUUCUUCU 
               
               
                   
               
               
                 &gt;hsa-mir-449c MI0003823 (SEQ ID NO: 1054) 
               
               
                 GCUGGGAUGUGUCAGGUAGGCAGUGUAUUGCUAGCGGCUGUUAAUGAUUUUAACAGUUGCUAGUUGCACUCCUCUCU 
               
               
                 GUUGCAUUCAGAAGC 
               
               
                   
               
               
                 &gt;hsa-mir-450a-1 MI0001652 (SEQ ID NO: 1055) 
               
               
                 AAACGAUACUAAACUGUUUUUGCGAUGUGUUCCUAAUAUGCACUAUAAAUAUAUUGGGAACAUUUUGCAUGUAUAGU 
               
               
                 UUUGUAUCAAUAUA 
               
               
                   
               
               
                 &gt;hsa-mir-450a-2 MI0003187 (SEQ ID NO: 1056) 
               
               
                 CCAAAGAAAGAUGCUAAACUAUUUUUGCGAUGUGUUCCUAAUAUGUAAUAUAAAUGUAUUGGGGACAUUUUGCAUUC 
               
               
                 AUAGUUUUGUAUCAAUAAUAUGG 
               
               
                   
               
               
                 &gt;hsa-mir-450b MI0005531 (SEQ ID NO: 1057) 
               
               
                 GCAGAAUUAUUUUUGCAAUAUGUUCCUGAAUAUGUAAUAUAAGUGUAUUGGGAUCAUUUUGCAUCCAUAGUUUUGUAU 
               
               
                   
               
               
                 &gt;hsa-mir-451a MI0001729 (SEQ ID NO: 1058) 
               
               
                 CUUGGGAAUGGCAAGGAAACCGUUACCAUUACUGAGUUUAGUAAUGGUAAUGGUUCUCUUGCUAUACCCAGA 
               
               
                   
               
               
                 &gt;hsa-mir-451b MI0017360 (SEQ ID NO: 1059) 
               
               
                 UGGGUAUAGCAAGAGAACCAUUACCAUUACUAAACUCAGUAAUGGUAACGGUUUCCUUGCCAUUCCCA 
               
               
                   
               
               
                 &gt;hsa-mir-452 MI0001733 (SEQ ID NO: 1060) 
               
               
                 GCUAAGCACUUACAACUGUUUGCAGAGGAAACUGAGACUUUGUAACUAUGUCUCAGUCUCAUCUGCAAAGAAGUAAG 
               
               
                 UGCUUUGC 
               
               
                   
               
               
                 &gt;hsa-mir-454 MI0003820 (SEQ ID NO: 1061) 
               
               
                 UCUGUUUAUCACCAGAUCCUAGAACCCUAUCAAUAUUGUCUCUGCUGUGUAAAUAGUUCUGAGUAGUGCAAUAUUGC 
               
               
                 UUAUAGGGUUUUGGUGUUUGGAAAGAACAAUGGGCAGG 
               
               
                   
               
               
                 &gt;hsa-mir-455 MI0003513 (SEQ ID NO: 1062) 
               
               
                 UCCCUGGCGUGAGGGUAUGUGCCUUUGGACUACAUCGUGGAAGCCAGCACCAUGCAGUCCAUGGGCAUAUACACUUG 
               
               
                 CCUCAAGGCCUAUGUCAUC 
               
               
                   
               
               
                 &gt;hsa-mir-466 MI0014157 (SEQ ID NO: 1063) 
               
               
                 GUGUGUGUAUAUGUGUGUUGCAUGUGUGUAUAUGUGUGUAUAUAUGUACACAUACACAUACACGCAACACACAUAUA 
               
               
                 UACAUGC 
               
               
                   
               
               
                 &gt;hsa-mir-483 MI0002467 (SEQ ID NO: 1064) 
               
               
                 GAGGGGGAAGACGGGAGGAAAGAAGGGAGUGGUUCCAUCACGCCUCCUCACUCCUCUCCUCCCGUCUUCUCCUCUC 
               
               
                   
               
               
                 &gt;hsa-mir-484 MI0002468 (SEQ ID NO: 1065) 
               
               
                 AGCCUCGUCAGGCUCAGUCCCCUCCCGAUAAACCCCUAAAUAGGGACUUUCCCGGGGGGUGACCCUGGCUUUUUUGG 
               
               
                 CG 
               
               
                   
               
               
                 &gt;hsa-mir-485 MI0002469 (SEQ ID NO: 1066) 
               
               
                 ACUUGGAGAGAGGCUGGCCGUGAUGAAUUCGAUUCAUCAAAGCGAGUCAUACACGGCUCUCCUCUCUUUUAGU 
               
               
                   
               
               
                 &gt;hsa-mir-486 MI0002470 (SEQ ID NO: 1067) 
               
               
                 GCAUCCUGUACUGAGCUGCCCCGAGGCCCUUCAUGCUGCCCAGCUCGGGGCAGCUCAGUACAGGAUAC 
               
               
                   
               
               
                 &gt;hsa-mir-487a MI0002471 (SEQ ID NO: 1068) 
               
               
                 GGUACUUGAAGAGUGGUUAUCCCUGCUGUGUUCGCUUAAUUUAUGACGAAUCAUACAGGGACAUCCAGUUUUUCAGU 
               
               
                 AUC 
               
               
                   
               
               
                 &gt;hsa-mir-487b MI0003530 (SEQ ID NO: 1069) 
               
               
                 UUGGUACUUGGAGAGUGGUUAUCCCUGUCCUGUUCGUUUUGCUCAUGUCGAAUCGUACAGGGUCAUCCACUUUUUCA 
               
               
                 GUAUCAA 
               
               
                   
               
               
                 &gt;hsa-mir-488 MI0003123 (SEQ ID NO: 1070) 
               
               
                 GAGAAUCAUCUCUCCCAGAUAAUGGCACUCUCAAACAAGUUUCCAAAUUGUUUGAAAGGCUAUUUCUUGGUCAGAUG 
               
               
                 ACUCUC 
               
               
                   
               
               
                 &gt;hsa-mir-489 MI0003124 (SEQ ID NO: 1071) 
               
               
                 GUGGCAGCUUGGUGGUCGUAUGUGUGACGCCAUUUACUUGAACCUUUAGGAGUGACAUCACAUAUACGGCAGCUAAA 
               
               
                 CUGCUAC 
               
               
                   
               
               
                 &gt;hsa-mir-490 MI0003125 (SEQ ID NO: 1072) 
               
               
                 UGGAGGCCUUGCUGGUUUGGAAAGUUCAUUGUUCGACACCAUGGAUCUCCAGGUGGGUCAAGUUUAGAGAUGCACCA 
               
               
                 ACCUGGAGGACUCCAUGCUGUUGAGCUGUUCACAAGCAGCGGACACUUCCA 
               
               
                   
               
               
                 &gt;hsa-mir-491 MI0003126 (SEQ ID NO: 1073) 
               
               
                 UUGACUUAGCUGGGUAGUGGGGAACCCUUCCAUGAGGAGUAGAACACUCCUUAUGCAAGAUUCCCUUCUACCUGGCU 
               
               
                 GGGUUGG 
               
               
                   
               
               
                 &gt;hsa-mir-492 MI0003131 (SEQ ID NO: 1074) 
               
               
                 CAACUACAGCCACUACUACAGGACCAUCGAGGACCUGCGGGACAAGAUUCUUGGUGCCACCAUUGAGAACGCCAGGA 
               
               
                 UUGUCCUGCAGAUCAACAAUGCUCAACUGGCUGCAGAUG 
               
               
                   
               
               
                 &gt;hsa-mir-493 MI0003132 (SEQ ID NO: 1075) 
               
               
                 CUGGCCUCCAGGGCUUUGUACAUGGUAGGCUUUCAUUCAUUCGUUUGCACAUUCGGUGAAGGUCUACUGUGUGCCAG 
               
               
                 GCCCUGUGCCAG 
               
               
                   
               
               
                 &gt;hsa-mir-494 MI0003134 (SEQ ID NO: 1076) 
               
               
                 GAUACUCGAAGGAGAGGUUGUCCGUGUUGUCUUCUCUUUAUUUAUGAUGAAACAUACACGGGAAACCUCUUUUUUAG 
               
               
                 UAUC 
               
               
                   
               
               
                 &gt;hsa-mir-495 MI0003135 (SEQ ID NO: 1077) 
               
               
                 UGGUACCUGAAAAGAAGUUGCCCAUGUUAUUUUCGCUUUAUAUGUGACGAAACAAACAUGGUGCACUUCUUUUUCGG 
               
               
                 UAUCA 
               
               
                   
               
               
                 &gt;hsa-mir-496 MI0003136 (SEQ ID NO: 1078) 
               
               
                 CCCAAGUCAGGUACUCGAAUGGAGGUUGUCCAUGGUGUGUUCAUUUUAUUUAUGAUGAGUAUUACAUGGCCAAUCUC 
               
               
                 CUUUCGGUACUCAAUUCUUCUUGGG 
               
               
                   
               
               
                 &gt;hsa-mir-497 MI0003138 (SEQ ID NO: 1079) 
               
               
                 CCACCCCGGUCCUGCUCCCGCCCCAGCAGCACACUGUGGUUUGUACGGCACUGUGGCCACGUCCAAACCACACUGUG 
               
               
                 GUGUUAGAGCGAGGGUGGGGGAGGCACCGCCGAGG 
               
               
                   
               
               
                 &gt;hsa-mir-498 MI0003142 (SEQ ID NO: 1080) 
               
               
                 AACCCUCCUUGGGAAGUGAAGCUCAGGCUGUGAUUUCAAGCCAGGGGGCGUUUUUCUAUAACUGGAUGAAAAGCACC 
               
               
                 UCCAGAGCUUGAAGCUCACAGUUUGAGAGCAAUCGUCUAAGGAAGUU 
               
               
                   
               
               
                 &gt;hsa-mir-499a MI0003183 (SEQ ID NO: 1081) 
               
               
                 GCCCUGUCCCCUGUGCCUUGGGCGGGCGGCUGUUAAGACUUGCAGUGAUGUUUAACUCCUCUCCACGUGAACAUCAC 
               
               
                 AGCAAGUCUGUGCUGCUUCCCGUCCCUACGCUGCCUGGGCAGGGU 
               
               
                   
               
               
                 &gt;hsa-mir-499b MI0017396 (SEQ ID NO: 1082) 
               
               
                 GGAAGCAGCACAGACUUGCUGUGAUGUUCACGUGGAGAGGAGUUAAACAUCACUGCAAGUCUUAACAGCCGCC 
               
               
                   
               
               
                 &gt;hsa-mir-500a MI0003184 (SEQ ID NO: 1083) 
               
               
                 GCUCCCCCUCUCUAAUCCUUGCUACCUGGGUGAGAGUGCUGUCUGAAUGCAAUGCACCUGGGCAAGGAUUCUGAGAG 
               
               
                 CGAGAGC 
               
               
                   
               
               
                 &gt;hsa-mir-500b MI0015903 (SEQ ID NO: 1084) 
               
               
                 CCCCCUCUCUAAUCCUUGCUACCUGGGUGAGAGUGCUUUCUGAAUGCAGUGCACCCAGGCAAGGAUUCUGCAAGGGG 
               
               
                 GA 
               
               
                   
               
               
                 &gt;hsa-mir-501 MI0003185 (SEQ ID NO: 1085) 
               
               
                 GCUCUUCCUCUCUAAUCCUUUGUCCCUGGGUGAGAGUGCUUUCUGAAUGCAAUGCACCCGGGCAAGGAUUCUGAGAG 
               
               
                 GGUGAGC 
               
               
                   
               
               
                 &gt;hsa-mir-502 MI0003186 (SEQ ID NO: 1086) 
               
               
                 UGCUCCCCCUCUCUAAUCCUUGCUAUCUGGGUGCUAGUGCUGGCUCAAUGCAAUGCACCUGGGCAAGGAUUCAGAGA 
               
               
                 GGGGGAGCU 
               
               
                   
               
               
                 &gt;hsa-mir-503 MI0003188 (SEQ ID NO: 1087) 
               
               
                 UGCCCUAGCAGCGGGAACAGUUCUGCAGUGAGCGAUCGGUGCUCUGGGGUAUUGUUUCCGCUGCCAGGGUA 
               
               
                   
               
               
                 &gt;hsa-mir-504 MI0003189 (SEQ ID NO: 1088) 
               
               
                 GCUGCUGUUGGGAGACCCUGGUCUGCACUCUAUCUGUAUUCUUACUGAAGGGAGUGCAGGGCAGGGUUUCCCAUACA 
               
               
                 GAGGGC 
               
               
                   
               
               
                 &gt;hsa-mir-505 MI0003190 (SEQ ID NO: 1089) 
               
               
                 GAUGCACCCAGUGGGGGAGCCAGGAAGUAUUGAUGUUUCUGCCAGUUUAGCGUCAACACUUGCUGGUUUCCUCUCUG 
               
               
                 GAGCAUC 
               
               
                   
               
               
                 &gt;hsa-mir-506 MI0003193 (SEQ ID NO: 1090) 
               
               
                 GCCACCACCAUCAGCCAUACUAUGUGUAGUGCCUUAUUCAGGAAGGUGUUACUUAAUAGAUUAAUAUUUGUAAGGCA 
               
               
                 CCCUUCUGAGUAGAGUAAUGUGCAACAUGGACAACAUUUGUGGUGGC 
               
               
                   
               
               
                 &gt;hsa-mir-507 MI0003194 (SEQ ID NO: 1091) 
               
               
                 GUGCUGUGUGUAGUGCUUCACUUCAAGAAGUGCCAUGCAUGUGUCUAGAAAUAUGUUUUGCACCUUUUGGAGUGAAA 
               
               
                 UAAUGCACAACAGAUAC 
               
               
                   
               
               
                 &gt;hsa-mir-508 MI0003195 (SEQ ID NO: 1092) 
               
               
                 CCACCUUCAGCUGAGUGUAGUGCCCUACUCCAGAGGGCGUCACUCAUGUAAACUAAAACAUGAUUGUAGCCUUUUGG 
               
               
                 AGUAGAGUAAUACACAUCACGUAACGCAUAUUUGGUGG 
               
               
                   
               
               
                 &gt;hsa-mir-509-1 MI0003196 (SEQ ID NO: 1093) 
               
               
                 CAUGCUGUGUGUGGUACCCUACUGCAGACAGUGGCAAUCAUGUAUAAUUAAAAAUGAUUGGUACGUCUGUGGGUAGA 
               
               
                 GUACUGCAUGACACAUG 
               
               
                   
               
               
                 &gt;hsa-mir-509-2 MI0005530 (SEQ ID NO: 1094) 
               
               
                 CAUGCUGUGUGUGGUACCCUACUGCAGACAGUGGCAAUCAUGUAUAAUUAAAAAUGAUUGGUACGUCUGUGGGUAGA 
               
               
                 GUACUGCAUGACAC 
               
               
                   
               
               
                 &gt;hsa-mir-509-3 MI0005717 (SEQ ID NO: 1095) 
               
               
                 GUGGUACCCUACUGCAGACGUGGCAAUCAUGUAUAAUUAAAAAUGAUUGGUACGUCUGUGGGUAGAGUACUGCAU 
               
               
                   
               
               
                 &gt;hsa-mir-510 MI0003197 (SEQ ID NO: 1096) 
               
               
                 GUGGUGUCCUACUCAGGAGAGUGGCAAUCACAUGUAAUUAGGUGUGAUUGAAACCUCUAAGAGUGGAGUAACAC 
               
               
                   
               
               
                 &gt;hsa-mir-511-1 MI0003127 (SEQ ID NO: 1097) 
               
               
                 CAAUAGACACCCAUCGUGUCUUUUGCUCUGCAGUCAGUAAAUAUUUUUUUGUGAAUGUGUAGCAAAAGACAGAAUGG 
               
               
                 UGGUCCAUUG 
               
               
                   
               
               
                 &gt;hsa-mir-511-2 MI0003128 (SEQ ID NO: 1098) 
               
               
                 CAAUAGACACCCAUCGUGUCUUUUGCUCUGCAGUCAGUAAAUAUUUUUUUGUGAAUGUGUAGCAAAAGACAGAAUGG 
               
               
                 UGGUCCAUUG 
               
               
                   
               
               
                 &gt;hsa-mir-512-1 MI0003140 (SEQ ID NO: 1099) 
               
               
                 UCUCAGUCUGUGGCACUCAGCCUUGAGGGCACUUUCUGGUGCCAGAAUGAAAGUGCUGUCAUAGCUGAGGUCCAAUG 
               
               
                 ACUGAGG 
               
               
                   
               
               
                 &gt;hsa-mir-512-2 MI0003141 (SEQ ID NO: 1100) 
               
               
                 GGUACUUCUCAGUCUGUGGCACUCAGCCUUGAGGGCACUUUCUGGUGCCAGAAUGAAAGUGCUGUCAUAGCUGAGGU 
               
               
                 CCAAUGACUGAGGCGAGCACC 
               
               
                   
               
               
                 &gt;hsa-mir-513a-1 MI0003191 (SEQ ID NO: 1101) 
               
               
                 GGGAUGCCACAUUCAGCCAUUCAGCGUACAGUGCCUUUCACAGGGAGGUGUCAUUUAUGUGAACUAAAAUAUAAAUU 
               
               
                 UCACCUUUCUGAGAAGGGUAAUGUACAGCAUGCACUGCAUAUGUGGUGUCCC 
               
               
                   
               
               
                 &gt;hsa-mir-513a-2 MI0003192 (SEQ ID NO: 1102) 
               
               
                 GGAUGCCACAUUCAGCCAUUCAGUGUGCAGUGCCUUUCACAGGGAGGUGUCAUUUAUGUGAACUAAAAUAUAAAUUU 
               
               
                 CACCUUUCUGAGAAGGGUAAUGUACAGCAUGCACUGCAUAUGUGGUGUCC 
               
               
                   
               
               
                 &gt;hsa-mir-513b MI0006648 (SEQ ID NO: 1103) 
               
               
                 GUGUACAGUGCCUUUCACAAGGAGGUGUCAUUUAUGUGAACUAAAAUAUAAAUGUCACCUUUUUGAGAGGAGUAAUG 
               
               
                 UACAGCA 
               
               
                   
               
               
                 &gt;hsa-mir-513c MI0006649 (SEQ ID NO: 1104) 
               
               
                 GCGUACAGUGCCUUUCUCAAGGAGGUGUCGUUUAUGUGAACUAAAAUAUAAAUUUCACCUUUCUGAGAAGAGUAAUG 
               
               
                 UACAGCA 
               
               
                   
               
               
                 &gt;hsa-mir-514a-1 MI0003198 (SEQ ID NO: 1105) 
               
               
                 AACAUGUUGUCUGUGGUACCCUACUCUGGAGAGUGACAAUCAUGUAUAAUUAAAUUUGAUUGACACUUCUGUGAGUA 
               
               
                 GAGUAACGCAUGACACGUACG 
               
               
                   
               
               
                 &gt;hsa-mir-514a-2 MI0003199 (SEQ ID NO: 1106) 
               
               
                 GUUGUCUGUGGUACCCUACUCUGGAGAGUGACAAUCAUGUAUAACUAAAUUUGAUUGACACUUCUGUGAGUAGAGUA 
               
               
                 ACGCAUGACAC 
               
               
                   
               
               
                 &gt;hsa-mir-514a-3 MI0003200 (SEQ ID NO: 1107) 
               
               
                 GUUGUCUGUGGUACCCUACUCUGGAGAGUGACAAUCAUGUAUAACUAAAUUUGAUUGACACUUCUGUGAGUAGAGUA 
               
               
                 ACGCAUGACAC 
               
               
                   
               
               
                 &gt;hsa-mir-514b MI0014251 (SEQ ID NO: 1108) 
               
               
                 CAUGUGGUACUCUUCUCAAGAGGGAGGCAAUCAUGUGUAAUUAGAUAUGAUUGACACCUCUGUGAGUGGAGUAACAC 
               
               
                 AUG 
               
               
                   
               
               
                 &gt;hsa-mir-515-1 MI0003144 (SEQ ID NO: 1109) 
               
               
                 UCUCAUGCAGUCAUUCUCCAAAAGAAAGCACUUUCUGUUGUCUGAAAGCAGAGUGCCUUCUUUUGGAGCGUUACUGU 
               
               
                 UUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-515-2 MI0003147 (SEQ ID NO: 1110) 
               
               
                 UCUCAUGCAGUCAUUCUCCAAAAGAAAGCACUUUCUGUUGUCUGAAAGCAGAGUGCCUUCUUUUGGAGCGUUACUGU 
               
               
                 UUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-516a-1 MI0003180 (SEQ ID NO: 1111) 
               
               
                 UCUCAGGCUGUGACCUUCUCGAGGAAAGAAGCACUUUCUGUUGUCUGAAAGAAAAGAAAGUGCUUCCUUUCAGAGGG 
               
               
                 UUACGGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-516a-2 MI0003181 (SEQ ID NO: 1112) 
               
               
                 UCUCAGGUUGUGACCUUCUCGAGGAAAGAAGCACUUUCUGUUGUCUGAAAGAAAAGAAAGUGCUUCCUUUCAGAGGG 
               
               
                 UUACGGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-516b-1 MI0003172 (SEQ ID NO: 1113) 
               
               
                 UCUCAGGCUGUGACCAUCUGGAGGUAAGAAGCACUUUCUGUUUUGUGAAAGAAAAGAAAGUGCUUCCUUUCAGAGGG 
               
               
                 UUACUCUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-516b-2 MI0003167 (SEQ ID NO: 1114) 
               
               
                 UCUCAUGAUGUGACCAUCUGGAGGUAAGAAGCACUUUGUGUUUUGUGAAAGAAAGUGCUUCCUUUCAGAGGGUUACU 
               
               
                 CUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-517a MI0003161 (SEQ ID NO: 1115) 
               
               
                 UCUCAGGCAGUGACCCUCUAGAUGGAAGCACUGUCUGUUGUAUAAAAGAAAAGAUCGUGCAUCCCUUUAGAGUGUUA 
               
               
                 CUGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-517b MI0003165 (SEQ ID NO: 1116) 
               
               
                 GUGACCCUCUAGAUGGAAGCACUGUCUGUUGUCUAAGAAAAGAUCGUGCAUCCCUUUAGAGUGUUAC 
               
               
                   
               
               
                 &gt;hsa-mir-517c MI0003174 (SEQ ID NO: 1117) 
               
               
                 GAAGAUCUCAGGCAGUGACCCUCUAGAUGGAAGCACUGUCUGUUGUCUAAGAAAAGAUCGUGCAUCCUUUUAGAGUG 
               
               
                 UUACUGUUUGAGAAAAUC 
               
               
                   
               
               
                 &gt;hsa-mir-518a-1 MI0003170 (SEQ ID NO: 1118) 
               
               
                 UCUCAAGCUGUGACUGCAAAGGGAAGCCCUUUCUGUUGUCUGAAAGAAGAGAAAGCGCUUCCCUUUGCUGGAUUACG 
               
               
                 GUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-518a-2 MI0003173 (SEQ ID NO: 1119) 
               
               
                 UCUCAAGCUGUGGGUCUGCAAAGGGAAGCCCUUUCUGUUGUCUAAAAGAAGAGAAAGCGCUUCCCUUUGCUGGAUUA 
               
               
                 CGGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-518b MI0003156 (SEQ ID NO: 1120) 
               
               
                 UCAUGCUGUGGCCCUCCAGAGGGAAGCGCUUUCUGUUGUCUGAAAGAAAACAAAGCGCUCCCCUUUAGAGGUUUACG 
               
               
                 GUUUGA 
               
               
                   
               
               
                 &gt;hsa-mir-518c MI0003159 (SEQ ID NO: 1121) 
               
               
                 GCGAGAAGAUCUCAUGCUGUGACUCUCUGGAGGGAAGCACUUUCUGUUGUCUGAAAGAAAACAAAGCGCUUCUCUUU 
               
               
                 AGAGUGUUACGGUUUGAGAAAAGC 
               
               
                   
               
               
                 &gt;hsa-mir-518d MI0003171 (SEQ ID NO: 1122) 
               
               
                 UCCCAUGCUGUGACCCUCUAGAGGGAAGCACUUUCUGUUGUCUGAAAGAAACCAAAGCGCUUCCCUUUGGAGCGUUA 
               
               
                 CGGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-518e MI0003169 (SEQ ID NO: 1123) 
               
               
                 UCUCAGGCUGUGACCCUCUAGAGGGAAGCGCUUUCUGUUGGCUAAAAGAAAAGAAAGCGCUUCCCUUCAGAGUGUUA 
               
               
                 ACGCUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-518f MI0003154 (SEQ ID NO: 1124) 
               
               
                 UCUCAUGCUGUGACCCUCUAGAGGGAAGCACUUUCUCUUGUCUAAAAGAAAAGAAAGCGCUUCUCUUUAGAGGAUUA 
               
               
                 CUCUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-519a-1 MI0003178 (SEQ ID NO: 1125) 
               
               
                 CUCAGGCUGUGACACUCUAGAGGGAAGCGCUUUCUGUUGUCUGAAAGAAAGGAAAGUGCAUCCUUUUAGAGUGUUAC 
               
               
                 UGUUUGAG 
               
               
                   
               
               
                 &gt;hsa-mir-519a-2 MI0003182 (SEQ ID NO: 1126) 
               
               
                 UCUCAGGCUGUGUCCCUCUACAGGGAAGCGCUUUCUGUUGUCUGAAAGAAAGGAAAGUGCAUCCUUUUAGAGUGUUA 
               
               
                 CUGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-519b MI0003151 (SEQ ID NO: 1127) 
               
               
                 CAUGCUGUGACCCUCUAGAGGGAAGCGCUUUCUGUUGUCUGAAAGAAAAGAAAGUGCAUCCUUUUAGAGGUUUACUG 
               
               
                 UUUG 
               
               
                   
               
               
                 &gt;hsa-mir-519c MI0003148 (SEQ ID NO: 1128) 
               
               
                 UCUCAGCCUGUGACCCUCUAGAGGGAAGCGCUUUCUGUUGUCUGAAAGAAAAGAAAGUGCAUCUUUUUAGAGGAUUA 
               
               
                 CAGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-519d MI0003162 (SEQ ID NO: 1129) 
               
               
                 UCCCAUGCUGUGACCCUCCAAAGGGAAGCGCUUUCUGUUUGUUUUCUCUUAAACAAAGUGCCUCCCUUUAGAGUGUU 
               
               
                 ACCGUUUGGGA 
               
               
                   
               
               
                 &gt;hsa-mir-519e MI0003145 (SEQ ID NO: 1130) 
               
               
                 UCUCAUGCAGUCAUUCUCCAAAAGGGAGCACUUUCUGUUUGAAAGAAAACAAAGUGCCUCCUUUUAGAGUGUUACUG 
               
               
                 UUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-520a MI0003149 (SEQ ID NO: 1131) 
               
               
                 CUCAGGCUGUGACCCUCCAGAGGGAAGUACUUUCUGUUGUCUGAGAGAAAAGAAAGUGCUUCCCUUUGGACUGUUUC 
               
               
                 GGUUUGAG 
               
               
                   
               
               
                 &gt;hsa-mir-520b MI0003155 (SEQ ID NO: 1132) 
               
               
                 CCCUCUACAGGGAAGCGCUUUCUGUUGUCUGAAAGAAAAGAAAGUGCUUCCUUUUAGAGGG 
               
               
                   
               
               
                 &gt;hsa-mir-520c MI0003158 (SEQ ID NO: 1133) 
               
               
                 UCUCAGGCUGUCGUCCUCUAGAGGGAAGCACUUUCUGUUGUCUGAAAGAAAAGAAAGUGCUUCCUUUUAGAGGGUUA 
               
               
                 CCGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-520d MI0003164 (SEQ ID NO: 1134) 
               
               
                 UCUCAAGCUGUGAGUCUACAAAGGGAAGCCCUUUCUGUUGUCUAAAAGAAAAGAAAGUGCUUCUCUUUGGUGGGUUA 
               
               
                 CGGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-520e MI0003143 (SEQ ID NO: 1135) 
               
               
                 UCUCCUGCUGUGACCCUCAAGAUGGAAGCAGUUUCUGUUGUCUGAAAGGAAAGAAAGUGCUUCCUUUUUGAGGGUUA 
               
               
                 CUGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-520f MI0003146 (SEQ ID NO: 1136) 
               
               
                 UCUCAGGCUGUGACCCUCUAAAGGGAAGCGCUUUCUGUGGUCAGAAAGAAAAGCAAGUGCUUCCUUUUAGAGGGUUA 
               
               
                 CCGUUUGGGA 
               
               
                   
               
               
                 &gt;hsa-mir-520g MI0003166 (SEQ ID NO: 1137) 
               
               
                 UCCCAUGCUGUGACCCUCUAGAGGAAGCACUUUCUGUUUGUUGUCUGAGAAAAAACAAAGUGCUUCCCUUUAGAGUG 
               
               
                 UUACCGUUUGGGA 
               
               
                   
               
               
                 &gt;hsa-mir-520h MI0003175 (SEQ ID NO: 1138) 
               
               
                 UCCCAUGCUGUGACCCUCUAGAGGAAGCACUUUCUGUUUGUUGUCUGAGAAAAAACAAAGUGCUUCCCUUUAGAGUU 
               
               
                 ACUGUUUGGGA 
               
               
                   
               
               
                 &gt;hsa-mir-521-1 MI0003176 (SEQ ID NO: 1139) 
               
               
                 UCUCAGGCUGUGACCCUCCAAAGGGAAGAACUUUCUGUUGUCUAAAAGAAAAGAACGCACUUCCCUUUAGAGUGUUA 
               
               
                 CCGUGUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-521-2 MI0003163 (SEQ ID NO: 1140) 
               
               
                 UCUCGGGCUGUGACUCUCCAAAGGGAAGAAUUUUCUCUUGUCUAAAAGAAAAGAACGCACUUCCCUUUAGAGUGUUA 
               
               
                 CCGUGUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-522 MI0003177 (SEQ ID NO: 1141) 
               
               
                 UCUCAGGCUGUGUCCCUCUAGAGGGAAGCGCUUUCUGUUGUCUGAAAGAAAAGAAAAUGGUUCCCUUUAGAGUGUUA 
               
               
                 CGCUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-523 MI0003153 (SEQ ID NO: 1142) 
               
               
                 UCUCAUGCUGUGACCCUCUAGAGGGAAGCGCUUUCUGUUGUCUGAAAGAAAAGAACGCGCUUCCCUAUAGAGGGUUA 
               
               
                 CCCUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-524 MI0003160 (SEQ ID NO: 1143) 
               
               
                 UCUCAUGCUGUGACCCUACAAAGGGAAGCACUUUCUCUUGUCCAAAGGAAAAGAAGGCGCUUCCCUUUGGAGUGUUA 
               
               
                 CGGUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-525 MI0003152 (SEQ ID NO: 1144) 
               
               
                 CUCAAGCUGUGACUCUCCAGAGGGAUGCACUUUCUCUUAUGUGAAAAAAAAGAAGGCGCUUCCCUUUAGAGCGUUAC 
               
               
                 GGUUUGGG 
               
               
                   
               
               
                 &gt;hsa-mir-526a-1 MI0003157 (SEQ ID NO: 1145) 
               
               
                 CUCAGGCUGUGACCCUCUAGAGGGAAGCACUUUCUGUUGCUUGAAAGAAGAGAAAGCGCUUCCUUUUAGAGGAUUAC 
               
               
                 UCUUUGAG 
               
               
                   
               
               
                 &gt;hsa-mir-526a-2 MI0003168 (SEQ ID NO: 1146) 
               
               
                 GUGACCCUCUAGAGGGAAGCACUUUCUGUUGAAAGAAAAGAACAUGCAUCCUUUCAGAGGGUUAC 
               
               
                   
               
               
                 &gt;hsa-mir-526b MI0003150 (SEQ ID NO: 1147) 
               
               
                 UCAGGCUGUGACCCUCUUGAGGGAAGCACUUUCUGUUGUCUGAAAGAAGAGAAAGUGCUUCCUUUUAGAGGCUUACU 
               
               
                 GUCUGA 
               
               
                   
               
               
                 &gt;hsa-mir-527 MI0003179 (SEQ ID NO: 1148) 
               
               
                 UCUCAAGCUGUGACUGCAAAGGGAAGCCCUUUCUGUUGUCUAAAAGAAAAGAAAGUGCUUCCCUUUGGUGAAUUACG 
               
               
                 GUUUGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-532 MI0003205 (SEQ ID NO: 1149) 
               
               
                 CGACUUGCUUUCUCUCCUCCAUGCCUUGAGUGUAGGACCGUUGGCAUCUUAAUUACCCUCCCACACCCAAGGCUUGC 
               
               
                 AAAAAAGCGAGCCU 
               
               
                   
               
               
                 &gt;hsa-mir-539 MI0003514 (SEQ ID NO: 1150) 
               
               
                 AUACUUGAGGAGAAAUUAUCCUUGGUGUGUUCGCUUUAUUUAUGAUGAAUCAUACAAGGACAAUUUCUUUUUGAGUAU 
               
               
                   
               
               
                 &gt;hsa-mir-541 MI0005539 (SEQ ID NO: 1151) 
               
               
                 ACGUCAGGGAAAGGAUUCUGCUGUCGGUCCCACUCCAAAGUUCACAGAAUGGGUGGUGGGCACAGAAUCUGGACUCU 
               
               
                 GCUUGUG 
               
               
                   
               
               
                 &gt;hsa-mir-542 MI0003686 (SEQ ID NO: 1152) 
               
               
                 CAGAUCUCAGACAUCUCGGGGAUCAUCAUGUCACGAGAUACCAGUGUGCACUUGUGACAGAUUGAUAACUGAAAGGU 
               
               
                 CUGGGAGCCACUCAUCUUCA 
               
               
                   
               
               
                 &gt;hsa-mir-543 MI0005565 (SEQ ID NO: 1153) 
               
               
                 UACUUAAUGAGAAGUUGCCCGUGUUUUUUUCGCUUUAUUUGUGACGAAACAUUCGCGGUGCACUUCUUUUUCAGUAUC 
               
               
                   
               
               
                 &gt;hsa-mir-544a MI0003515 (SEQ ID NO: 1154) 
               
               
                 AUUUUCAUCACCUAGGGAUCUUGUUAAAAAGCAGAUUCUGAUUCAGGGACCAAGAUUCUGCAUUUUUAGCAAGUUCU 
               
               
                 CAAGUGAUGCUAAU 
               
               
                   
               
               
                 &gt;hsa-mir-544b MI0014159 (SEQ ID NO: 1155) 
               
               
                 GGAAUUUUGUUAAAAUGCAGAAUCCAUUUCUGUAGCUCUGAGACUAGACCUGAGGUUGUGCAUUUCUAACAAAGUGCC 
               
               
                   
               
               
                 &gt;hsa-mir-545 MI0003516 (SEQ ID NO: 1156) 
               
               
                 CCCAGCCUGGCACAUUAGUAGGCCUCAGUAAAUGUUUAUUAGAUGAAUAAAUGAAUGACUCAUCAGCAAACAUUUAU 
               
               
                 UGUGUGCCUGCUAAAGUGAGCUCCACAGG 
               
               
                   
               
               
                 &gt;hsa-mir-548a-1 MI0003593 (SEQ ID NO: 1157) 
               
               
                 UGCAGGGAGGUAUUAAGUUGGUGCAAAAGUAAUUGUGAUUUUUGCCAUUAAAAGUAACGACAAAACUGGCAAUUACU 
               
               
                 UUUGCACCAAACCUGGUAUU 
               
               
                   
               
               
                 &gt;hsa-mir-548a-2 MI0003598 (SEQ ID NO: 1158) 
               
               
                 UGUGAUGUGUAUUAGGUUUGUGCAAAAGUAAUUGGGGUUUUUUGCCGUUAAAAGUAAUGGCAAAACUGGCAAUUACU 
               
               
                 UUUGCACCAAACUAAUAUAA 
               
               
                   
               
               
                 &gt;hsa-mir-548a-3 MI0003612 (SEQ ID NO: 1159) 
               
               
                 CCUAGAAUGUUAUUAGGUCGGUGCAAAAGUAAUUGCGAGUUUUACCAUUACUUUCAAUGGCAAAACUGGCAAUUACU 
               
               
                 UUUGCACCAACGUAAUACUU 
               
               
                   
               
               
                 &gt;hsa-mir-548aa-1 MI0016689 (SEQ ID NO: 1160) 
               
               
                 CUUUAUUAGUCUGGUGCAAAAGAAACUGUGGUUUUUGCCAUUACUUUUACAGGCAAAAACCACAAUUACUUUUGCAC 
               
               
                 CAACCUAAUAUAACUUGUUU 
               
               
                   
               
               
                 &gt;hsa-mir-548aa-2 MI0016690 (SEQ ID NO: 1161) 
               
               
                 UUUUAUUAGGUUGGUGCAAAAGAAACUGUGGUUUUUGCCAUUACUUUCAAUGGCAAAAACCACAAUUACUUUUGCAC 
               
               
                 CAACCUAAAUCUUCCCUCUC 
               
               
                   
               
               
                 &gt;hsa-mir-548ab MI0016752 (SEQ ID NO: 1162) 
               
               
                 AUGUUGGUGCAAAAGUAAUUGUGGAUUUUGCUAUUACUUGUAUUUAUUUGUAAUGCAAAACCCGCAAUUAGUUUUGC 
               
               
                 ACCAACC 
               
               
                   
               
               
                 &gt;hsa-mir-548ac MI0016762 (SEQ ID NO: 1163) 
               
               
                 GUAUUAGGUUGGUGCAAAAGUUAUUGUGGUUUUUGCUAUUUUUUUUUAAUGGCAAAAACCGGCAAUUACUUUUGCAC 
               
               
                 UAACCUAGUAG 
               
               
                   
               
               
                 &gt;hsa-mir-548ad MI0016770 (SEQ ID NO: 1164) 
               
               
                 CUGUUAGGUUGGUGCAAAAGUAAUUGUGGUUUUUGAAAGUAACUUGGCGAAAACGACAAUGACUUUUGCACCAAUCU 
               
               
                 AAUAC 
               
               
                   
               
               
                 &gt;hsa-mir-548ae-1 MI0016779 (SEQ ID NO: 1165) 
               
               
                 GCAGUUUUUGCCAUUAAGUUGCGGUUUUUGCCAUUAUAAUGGCAAAAACUGCAAUUACUUUCACACCUGC 
               
               
                   
               
               
                 &gt;hsa-mir-548ae-2 MI0016780 (SEQ ID NO: 1166) 
               
               
                 UGUGCAAAAGUAAUUGUGGUUUUUGUCAUUUAAAAGUAAUGGCAAAAACUGCAAUUACUUUCACACC 
               
               
                   
               
               
                 &gt;hsa-mir-548ag-1 MI0016793 (SEQ ID NO: 1167) 
               
               
                 GUGCAAAGGUAAUUGUGGUUUCUGCUUUUAAAGGUAAUGGCAAAUAUUACAUUUACUUUUGCACCA 
               
               
                   
               
               
                 &gt;hsa-mir-548ag-2 MI0016794 (SEQ ID NO: 1168) 
               
               
                 UGCAAAGGUAAUUGUGGUUUCUGCCAUUGAAAGUAAAGGCAAGAACCUCAAUUACCUUUGCAGC 
               
               
                   
               
               
                 &gt;hsa-mir-548ah MI0016796 (SEQ ID NO: 1169) 
               
               
                 AGGUUGGUGCAAAAGUGAUUGCAGUGUUUGCCAAUAAAAGUAAUGACAAAAACUGCAGUUACUUUUGCACCAGCCC 
               
               
                   
               
               
                 &gt;hsa-mir-548ai MI0016813 (SEQ ID NO: 1170) 
               
               
                 GUAUUAGGUUGGUGCAAAGGUAAUUGCAGUUUUUCCCAUUUAAAAUAUGGAAAAAAAAAUCACAAUUACUUUUGCAU 
               
               
                 CAACCUAAUAA 
               
               
                   
               
               
                 &gt;hsa-mir-548aj-1 MI0016814 (SEQ ID NO: 1171) 
               
               
                 AUUGGUGUAAAAGUAAUUGCAGGUUAUGCCAUUAAAAGUAAUGGUAAAAACUGCAAUUACUUUUACACUAAC 
               
               
                   
               
               
                 &gt;hsa-mir-548aj-2 MI0016815 (SEQ ID NO: 1172) 
               
               
                 AAGGUAUUAGGUUGGUGCAAAAGUAAUUGCAGUUUUUGCUAUUACUUUUAAUGGUAAAAACUGCAAUUACUUUUACA 
               
               
                 CCAACCUAAUAUUUA 
               
               
                   
               
               
                 &gt;hsa-mir-548ak MI0016840 (SEQ ID NO: 1173) 
               
               
                 GUGCAAAAGUAACUGCGGUUUUUGAGAAGUAAUUGAAAACCGCAAUUACUUUUGCAG 
               
               
                   
               
               
                 &gt;hsa-mir-548a1 MI0016851 (SEQ ID NO: 1174) 
               
               
                 GGUCGGUGCAAAAGUAAUUGCUGUUUUUGCCAUUAAAAAUAAUGGCAUUAAAAGUAAUGGCAAAAACGGCAAUGACU 
               
               
                 UUUGUACCAAUCUAAUAUCU 
               
               
                   
               
               
                 &gt;hsa-mir-548am MI0016904 (SEQ ID NO: 1175) 
               
               
                 AGUUGGUGCAAAAGUAAUUGCGGUUUUUGCCGUCGAAAAUAAUGGCAAAAACUGCAGUUACUUUUGUACCAAUG 
               
               
                   
               
               
                 &gt;hsa-mir-548an MI0016907 (SEQ ID NO: 1176) 
               
               
                 CAUUAGGUUGGUGCAAAAGGCAUUGUGGUUUUUGCCUAUAAAAGUAAUGGCAAAAACCGCAAUUCCUUUUGCACCAA 
               
               
                 CCUAAU 
               
               
                   
               
               
                 &gt;hsa-mir-548ao MI0017871 (SEQ ID NO: 1177) 
               
               
                 AACUAUUCUUAGGUUGAUGCAGAAGUAACUACGGUUUUUGCAGUUGAAAGUAAUGGCAAAGACCGUGACUACUUUUG 
               
               
                 CAACAGCCUAAUAGUUUCU 
               
               
                   
               
               
                 &gt;hsa-mir-548ap MI0017875 (SEQ ID NO: 1178) 
               
               
                 ACCAAUUCCUAGGUUGGUGCAAAAGUAAUUGCGGUCUUUGUCAUUAAAACCAAUAACAAAAACCACAAUUACUUUUU 
               
               
                 ACUGACCUAAAGAUUAAUU 
               
               
                   
               
               
                 &gt;hsa-mir-548aq MI0019130 (SEQ ID NO: 1179) 
               
               
                 GAAAGUAAUUGCUGUUUUUGCCAUUACUUUCAGUGGCAAAAACUGCAAUUACUUUUGC 
               
               
                   
               
               
                 &gt;hsa-mir-548ar MI0019131 (SEQ ID NO: 1180) 
               
               
                 AAAAGUAAUUGCAGUUUUUGCUGUUGAACGUAGUGGUAAAACUGCAGUUAUUUUUGC 
               
               
                   
               
               
                 &gt;hsa-mir-548as MI0019132 (SEQ ID NO: 1181) 
               
               
                 AAAAGUAAUUGCGGGUUUUGCCGUUGCUUUUAAUGGUAAAACCCACAAUUAUGUUUGU 
               
               
                   
               
               
                 &gt;hsa-mir-548at MI0019137 (SEQ ID NO: 1182) 
               
               
                 AAAAGUUAUUGCGGUUUUGGCUGCCAAAAGAAAUGGCCAAAACCGCAGUAACUUUUGU 
               
               
                   
               
               
                 &gt;hsa-mir-548au MI0019145 (SEQ ID NO: 1183) 
               
               
                 AAAAGUAAUUGCGGUUUUUGCUAUUGGUUUUAAUGGCAGUUACUUUUGCACCAG 
               
               
                   
               
               
                 &gt;hsa-mir-548av MI0019152 (SEQ ID NO: 1184) 
               
               
                 AAAAGUACUUGCGGAUUUGCCAUCACCUUUACCUUUAAUGGCAAAACUGCAGUUACUUUUGC 
               
               
                   
               
               
                 &gt;hsa-mir-548aw MI0019283 (SEQ ID NO: 1185) 
               
               
                 UAGGUCGGUGCAAAAGUCAUCACGGUUUUUACCAUUAAAACCGCGAUGACUUUUGCAUCAACCUA 
               
               
                   
               
               
                 &gt;hsa-mir-548ax MI0019286 (SEQ ID NO: 1186) 
               
               
                 GAUUGGUGCAGAAGUAAUUGCGGUUUUGCCAUGGAAAGUAAUGGCAAAAACCGUAAUUACUUUUGUACCAACC 
               
               
                   
               
               
                 &gt;hsa-mir-548b MI0003596 (SEQ ID NO: 1187) 
               
               
                 CAGACUAUAUAUUUAGGUUGGCGCAAAAGUAAUUGUGGUUUUGGCCUUUAUUUUCAAUGGCAAGAACCUCAGUUGCU 
               
               
                 UUUGUGCCAACCUAAUACUU 
               
               
                   
               
               
                 &gt;hsa-mir-548c MI0003630 (SEQ ID NO: 1188) 
               
               
                 CAUUGGCAUCUAUUAGGUUGGUGCAAAAGUAAUUGCGGUUUUUGCCAUUACUUUCAGUAGCAAAAAUCUCAAUUACU 
               
               
                 UUUGCACCAACUUAAUACUU 
               
               
                   
               
               
                 &gt;hsa-mir-548d-1 MI0003668 (SEQ ID NO: 1189) 
               
               
                 AAACAAGUUAUAUUAGGUUGGUGCAAAAGUAAUUGUGGUUUUUGCCUGUAAAAGUAAUGGCAAAAACCACAGUUUCU 
               
               
                 UUUGCACCAGACUAAUAAAG 
               
               
                   
               
               
                 &gt;hsa-mir-548d-2 MI0003671 (SEQ ID NO: 1190) 
               
               
                 GAGAGGGAAGAUUUAGGUUGGUGCAAAAGUAAUUGUGGUUUUUGCCAUUGAAAGUAAUGGCAAAAACCACAGUUUCU 
               
               
                 UUUGCACCAACCUAAUAAAA 
               
               
                   
               
               
                 &gt;hsa-mir-548e MI0006344 (SEQ ID NO: 1191) 
               
               
                 UUAUUAGGUUGGUACAAAAGCAAUCGCGGUUUUUGCUAUUACUUUUAAAGGCAAAAACUGAGACUACUUUUGCACCA 
               
               
                 ACCUGAUAGAA 
               
               
                   
               
               
                 &gt;hsa-mir-548f-1 MI0006374 (SEQ ID NO: 1192) 
               
               
                 AUUAGGUUGGUGCAAAAGUAAUCACAGUUUUUGACAUUACUUUCAAAGACAAAAACUGUAAUUACUUUUGGACCAAC 
               
               
                 CUAAUAG 
               
               
                   
               
               
                 &gt;hsa-mir-548f-2 MI0006375 (SEQ ID NO: 1193) 
               
               
                 UAAUAACUAUUAGGUUGGUGCGAACAUAAUUGCAGUUUUUAUCAUUACUUUUAAUGGCAAAAACUGUAAUUACUUUU 
               
               
                 GCACCAACCUAAUAUUUUAGU 
               
               
                   
               
               
                 &gt;hsa-mir-548f-3 MI0006376 (SEQ ID NO: 1194) 
               
               
                 AUUAGGUUGGUGCAAACCUAAUUGCAAUUUUUGCAGUUUUUUUAAGUAAUUGCAAAAACUGUAAUUACUUUUGCACC 
               
               
                 AACCUAAUAC 
               
               
                   
               
               
                 &gt;hsa-mir-548f-4 MI0006377 (SEQ ID NO: 1195) 
               
               
                 GAGUUCUAACGUAUUAGGUUGGUGCAAAAGUAAUAGUGGUUUUUGCCAUUAAAAGUAAUGACAAAAACUGUAAUUAC 
               
               
                 UUUUGGAACAAUAUUAAUAGAAUUUCAG 
               
               
                   
               
               
                 &gt;hsa-mir-548f-5 MI0006378 (SEQ ID NO: 1196) 
               
               
                 UAUUAGGUUGCUGCAAAAGUAAUCAUGUUUUUUUCCAUUGUAAGUAAUGGGAAAAACUGUAAUUACUUUUGUACCAA 
               
               
                 CCUAAUAGC 
               
               
                   
               
               
                 &gt;hsa-mir-548g MI0006395 (SEQ ID NO: 1197) 
               
               
                 AGUUAUUAGAUUAGUGCAAAAGUAAUUGCAGUUUUUGCAUUACGUUCUAUGGCAAAACUGUAAUUACUUUUGUACCA 
               
               
                 ACAUAAUACUUC 
               
               
                   
               
               
                 &gt;hsa-mir-548h-1 MI0006411 (SEQ ID NO: 1198) 
               
               
                 UCUGUCCAUUAGGUGGGUGCAAAAGUAAUCGCGGUUUUUGUCAUUACUUUUAAUGGUAAAAACUGGAAUUACUUUUG 
               
               
                 CACUGACCUAAUAUUAAGCCAGAUA 
               
               
                   
               
               
                 &gt;hsa-mir-548h-2 MI0006412 (SEQ ID NO: 1199) 
               
               
                 GUAUUAGGUUGGUGCAAAAGUAAUCGCGGUUUUUGUCAUUACUUUCAAUGGCAAACACCACAAUUACUUUUGCACCA 
               
               
                 ACCUAAUAUAA 
               
               
                   
               
               
                 &gt;hsa-mir-548h-3 MI0006413 (SEQ ID NO: 1200) 
               
               
                 UCUGAUUCUGCAUGUAUUAGGUUGGUGCAAAAGUAAUCGCGGUUUUUGUCAUUGAAAGUAAUAGCAAAAACUGCAAU 
               
               
                 UACUUUUGCACCAACCUAAAAGUAGUCACUGUCUUCAGAUA 
               
               
                   
               
               
                 &gt;hsa-mir-548h-4 MI0006414 (SEQ ID NO: 1201) 
               
               
                 GCUAUUAGGUUGGUGCAAAAGUAAUCGCGGUUUUUGUCAUUACUUUAAUUACUUUACGUUUCAUUAAUGACAAAAAC 
               
               
                 CGCAAUUACUUUUGCACCAACCUAAUACUUGCUA 
               
               
                   
               
               
                 &gt;hsa-mir-548h-5 MI0016751 (SEQ ID NO: 1202) 
               
               
                 ACAAAAGUAAUCGCGGUUUUUGUCAUUACUUUUAACUGUAAAAACCACGGUUGCUUUUGC 
               
               
                   
               
               
                 &gt;hsa-mir-548i-1 MI0006421 (SEQ ID NO: 1203) 
               
               
                 CAGAUGGCUCUGAAGUUUGCACCCUAUUAGGUUGGUGCAAAAGUAAUUGCGGAUUUUGCCAUUAAAAGUAAUGGCAA 
               
               
                 AAAUAGCAAUUAUUUUUGUACCAGCCUAGUAUCUUUUCUCCUUCUACCAAACUUUGUCCCUGAGCCAUCUCA 
               
               
                   
               
               
                 &gt;hsa-mir-548i-2 MI0006422 (SEQ ID NO: 1204) 
               
               
                 UAGAUGGCUCCGAAGUUUGCAUCCUAUUAGUUUGGUGCAAAAGUAAUUGCGGAUUUUGCCAUUAAAAGUAAUGGCAA 
               
               
                 AAAUAGCAAUUAUUUUUGUACCAGCCUAGUAUCUUUUCUCCUUCUAACAAAGUUCGUCCCUGAUCCAUCUCA 
               
               
                   
               
               
                 &gt;hsa-mir-548i-3 MI0006423 (SEQ ID NO: 1205) 
               
               
                 CAGAUGGCUCCGAAGUUUACAUCCUAUUAGGUUUGUGCAAAAGUAAUUGCGGAUUUUGCCAUUAAAAGUAAUGGCAA 
               
               
                 AAAUAGCAAUUAUUUUUGUACCAGCCUAGUAUCUUUUCUCCUUCUACCAAACUUUGUCCCUGAGCCAUCUCA 
               
               
                   
               
               
                 &gt;hsa-mir-548i-4 MI0006424 (SEQ ID NO: 1206) 
               
               
                 AGGUUGGUGCAAAAGUAAUUGCGGAUUUUGCCAUACUUUUAACGGCAAAAACCACAAAUAUUAUUGCACCAACCUAU 
               
               
                   
               
               
                 &gt;hsa-mir-548j MI0006345 (SEQ ID NO: 1207) 
               
               
                 GGGCAGCCAGUGAAUAGUUAGCUGGUGCAAAAGUAAUUGCGGUCUUUGGUAUUACUUUCAGUGGCAAAAACUGCAUU 
               
               
                 ACUUUUGCACCAGCCUACUAGAACGCUGAGUUCAG 
               
               
                   
               
               
                 &gt;hsa-mir-548k MI0006354 (SEQ ID NO: 1208) 
               
               
                 CUUUUCUCAAGUAUUGCUGUUAGGUUGGUGCAAAAGUACUUGCGGAUUUUGCUUUACUUUUAAUGGCAAAAACCGCA 
               
               
                 AUUAUUUUUGCUUCAACCUAAUAUGAUGCAAAAUUGGCU 
               
               
                   
               
               
                 &gt;hsa-mir-5481 MI0006361 (SEQ ID NO: 1209) 
               
               
                 UAUUAGGUUGGUGCAAAAGUAUUUGCGGGUUUUGUCGUAGAAAGUAAUGGCAAAAACUGCAGUUACUUGUGCACCAA 
               
               
                 CCAAAUGCU 
               
               
                   
               
               
                 &gt;hsa-mir-548m MI0006400 (SEQ ID NO: 1210) 
               
               
                 AUAUUAGGUUGGUGCAAAGGUAUUUGUGGUUUUUGUCAUUAAAGUAAUGCAAAAGCCACAAAUACCUUUGCACCAAC 
               
               
                 CUAAUAUUA 
               
               
                   
               
               
                 &gt;hsa-mir-548n MI0006399 (SEQ ID NO: 1211) 
               
               
                 AGGUUGGUGCAAAAGUAAUUGUGGAUUUUGUCGUUAAAAAUAGCAAAACCCGCAAUUACUUUUGCACCAACCUAA 
               
               
                   
               
               
                 &gt;hsa-mir-548o MI0006402 (SEQ ID NO: 1212) 
               
               
                 UGGUGAAAAUGUGUUGAUUGUAAUGGUUCCUAUUCUGAUCAAUAAACAUGGUUUGAGCCUAGUUACAAUGAUCUAAA 
               
               
                 AUUCACGGUCCAAAACUGCAGUUACUUUUGCACCAAC 
               
               
                   
               
               
                 &gt;hsa-mir-548o-2 MI0016746 (SEQ ID NO: 1213) 
               
               
                 UGGUGCAAAAGUAAUUGCGGUUUUUGCCAUUAAAAGUAAUGCGGCCAAAACUGCAGUUACUUUUGCACCC 
               
               
                   
               
               
                 &gt;hsa-mir-548p MI0006420 (SEQ ID NO: 1214) 
               
               
                 AUUAGGUUGGUAUAAAAUUAAUUGCAGUUUUUGUCAUUACUUUCAAUAGCAAAAACUGCAGUUACUUUUGCACCAAU 
               
               
                 GUAAUAC 
               
               
                   
               
               
                 &gt;hsa-mir-548q MI0010637 (SEQ ID NO: 1215) 
               
               
                 AUAUUAGGCUGGUGCAAAAGUAAUGGCGGUUUUUGCCAUUACUUUUCAUUUUUACCAUUAAAAGUAAUGGCAAAAAG 
               
               
                 CAUGAUUACUUUUUCACCAACCU 
               
               
                   
               
               
                 &gt;hsa-mir-548s MI0014141 (SEQ ID NO: 1216) 
               
               
                 UUGCUGCAAAAAUAAUUGCAGUUUUUGCCAUUAUUUUUAAUAAUUAUAAUAAUGGCCAAAACUGCAGUUAUUUUUGC 
               
               
                 ACCAA 
               
               
                   
               
               
                 &gt;hsa-mir-548t MI0014164 (SEQ ID NO: 1217) 
               
               
                 AGGGUGGUGCAAAAGUGAUCGUGGUUUUUGCAAUUUUUUAAUGACAAAAACCACAAUUACUUUUGCACCAACCU 
               
               
                   
               
               
                 &gt;hsa-mir-548u MI0014168 (SEQ ID NO: 1218) 
               
               
                 AUUAGGAUGGUGCAAAAGUAAUGUGGUUUUUUUCUUUACUUUUAAUGGCAAAGACUGCAAUUACUUUUGCGCCAACC 
               
               
                 UAAU 
               
               
                   
               
               
                 &gt;hsa-mir-548v MI0014174 (SEQ ID NO: 1219) 
               
               
                 AAUACUAGGUUUGAGCAAAAGUAAUUGCGGUUUUGCCAUCAUGCCAAAAGCUACAGUUACUUUUGCACCAGCCUAAU 
               
               
                 AUU 
               
               
                   
               
               
                 &gt;hsa-mir-548w MI0014222 (SEQ ID NO: 1220) 
               
               
                 GGUUGGUGCAAAAGUAACUGCGGUUUUUGCCUUUCAACAUAAUGGCAAAACCCACAAUUACUUUUGCACCAAUC 
               
               
                   
               
               
                 &gt;hsa-mir-548x MI0014244 (SEQ ID NO: 1221) 
               
               
                 AGGUUAGUGCAAAAGUAAUUGCAGUUUUUGCGUUACUUUCAAUCGUAAAAACUGCAAUUACUUUCACACCAAUCU 
               
               
                   
               
               
                 &gt;hsa-mir-548x-2 MI0016833 (SEQ ID NO: 1222) 
               
               
                 AUGCCAAAUAUUAGGUUGGCACAAAAGUAAUUGUGGCUUUUGCCAUUAAAAGUAAUGGUAAAAACUGCAAUUACUUU 
               
               
                 CGUGCCAACCUAAUAUUUGUGUG 
               
               
                   
               
               
                 &gt;hsa-mir-548y MI0016595 (SEQ ID NO: 1223) 
               
               
                 GCCUAAACUAUUAGGUUGGUGCAAAAGUAAUCACUGUUUUUGCCAUUACUCUCAGUGGCAAAAACCGUGAUUACUUU 
               
               
                 UGCACCAACCUAGUAACACCUUCACUGUGGGGG 
               
               
                   
               
               
                 &gt;hsa-mir-548z MI0016688 (SEQ ID NO: 1224) 
               
               
                 AAGUAUUAAGUUGGUGCAAAAGUAAUUGAGAUUUUUGCUACUGAAAGUAAUGGCAAAAACCGCAAUUACUUUUGCAC 
               
               
                 CAACCUAAUAGAUGCCAAUG 
               
               
                   
               
               
                 &gt;hsa-mir-549 MI0003679 (SEQ ID NO: 1225) 
               
               
                 AGACAUGCAACUCAAGAAUAUAUUGAGAGCUCAUCCAUAGUUGUCACUGUCUCAAAUCAGUGACAACUAUGGAUGAG 
               
               
                 CUCUUAAUAUAUCCCAGGC 
               
               
                   
               
               
                 &gt;hsa-mir-550a-1 MI0003600 (SEQ ID NO: 1226) 
               
               
                 UGAUGCUUUGCUGGCUGGUGCAGUGCCUGAGGGAGUAAGAGCCCUGUUGUUGUAAGAUAGUGUCUUACUCCCUCAGG 
               
               
                 CACAUCUCCAACAAGUCUCU 
               
               
                   
               
               
                 &gt;hsa-mir-550a-2 MI0003601 (SEQ ID NO: 1227) 
               
               
                 UGAUGCUUUGCUGGCUGGUGCAGUGCCUGAGGGAGUAAGAGCCCUGUUGUUGUCAGAUAGUGUCUUACUCCCUCAGG 
               
               
                 CACAUCUCCAGCGAGUCUCU 
               
               
                   
               
               
                 &gt;hsa-mir-550a-3 MI0003762 (SEQ ID NO: 1228) 
               
               
                 GAUGCUUUGCUGGCUGGUGCAGUGCCUGAGGGAGUAAGAGUCCUGUUGUUGUAAGAUAGUGUCUUACUCCCUCAGGC 
               
               
                 ACAUCUCCAACAAGUCUC 
               
               
                   
               
               
                 &gt;hsa-mir-550b-1 MI0016686 (SEQ ID NO: 1229) 
               
               
                 AGAGACUUGUUGGAGAUGUGCCUGAGGGAGUAAGACACUAUCUUACAACAACAGGGCUCUUACUCCCUCAGGCACUG 
               
               
                 CACCAGCCAGCAAAGCAUCA 
               
               
                   
               
               
                 &gt;hsa-mir-550b-2 MI0016687 (SEQ ID NO: 1230) 
               
               
                 AGAGACUCGCUGGAGAUGUGCCUGAGGGAGUAAGACACUAUCUGACAACAACAGGGCUCUUACUCCCUCAGGCACUG 
               
               
                 CACCAGCCAGCAAAGCAUCA 
               
               
                   
               
               
                 &gt;hsa-mir-551a MI0003556 (SEQ ID NO: 1231) 
               
               
                 GGGGACUGCCGGGUGACCCUGGAAAUCCAGAGUGGGUGGGGCCAGUCUGACCGUUUCUAGGCGACCCACUCUUGGUU 
               
               
                 UCCAGGGUUGCCCUGGAAA 
               
               
                   
               
               
                 &gt;hsa-mir-551b MI0003575 (SEQ ID NO: 1232) 
               
               
                 AGAUGUGCUCUCCUGGCCCAUGAAAUCAAGCGUGGGUGAGACCUGGUGCAGAACGGGAAGGCGACCCAUACUUGGUU 
               
               
                 UCAGAGGCUGUGAGAAUAA 
               
               
                   
               
               
                 &gt;hsa-mir-552 MI0003557 (SEQ ID NO: 1233) 
               
               
                 AACCAUUCAAAUAUACCACAGUUUGUUUAACCUUUUGCCUGUUGGUUGAAGAUGCCUUUCAACAGGUGACUGGUUAG 
               
               
                 ACAAACUGUGGUAUAUACA 
               
               
                   
               
               
                 &gt;hsa-mir-553 MI0003558 (SEQ ID NO: 1234) 
               
               
                 CUUCAAUUUUAUUUUAAAACGGUGAGAUUUUGUUUUGUCUGAGAAAAUCUCGCUGUUUUAGACUGAGG 
               
               
                   
               
               
                 &gt;hsa-mir-554 MI0003559 (SEQ ID NO: 1235) 
               
               
                 ACCUGAGUAACCUUUGCUAGUCCUGACUCAGCCAGUACUGGUCUUAGACUGGUGAUGGGUCAGGGUUCAUAUUUUGG 
               
               
                 CAUCUCUCUCUGGGCAUCU 
               
               
                   
               
               
                 &gt;hsa-mir-555 MI0003561 (SEQ ID NO: 1236) 
               
               
                 GGAGUGAACUCAGAUGUGGAGCACUACCUUUGUGAGCAGUGUGACCCAAGGCCUGUGGACAGGGUAAGCUGAACCUC 
               
               
                 UGAUAAAACUCUGAUCUAU 
               
               
                   
               
               
                 &gt;hsa-mir-556 MI0003562 (SEQ ID NO: 1237) 
               
               
                 GAUAGUAAUAAGAAAGAUGAGCUCAUUGUAAUAUGAGCUUCAUUUAUACAUUUCAUAUUACCAUUAGCUCAUCUUUU 
               
               
                 UUAUUACUACCUUCAACA 
               
               
                   
               
               
                 &gt;hsa-mir-557 MI0003563 (SEQ ID NO: 1238) 
               
               
                 AGAAUGGGCAAAUGAACAGUAAAUUUGGAGGCCUGGGGCCCUCCCUGCUGCUGGAGAAGUGUUUGCACGGGUGGGCC 
               
               
                 UUGUCUUUGAAAGGAGGUGGA 
               
               
                   
               
               
                 &gt;hsa-mir-558 MI0003564 (SEQ ID NO: 1239) 
               
               
                 GUGUGUGUGUGUGUGUGUGGUUAUUUUGGUAUAGUAGCUCUAGACUCUAUUAUAGUUUCCUGAGCUGCUGUACCAAA 
               
               
                 AUACCACAAACGGGCUG 
               
               
                   
               
               
                 &gt;hsa-mir-559 MI0003565 (SEQ ID NO: 1240) 
               
               
                 GCUCCAGUAACAUCUUAAAGUAAAUAUGCACCAAAAUUACUUUUGGUAAAUACAGUUUUGGUGCAUAUUUACUUUAG 
               
               
                 GAUGUUACUGGAGCUCCCA 
               
               
                   
               
               
                 &gt;hsa-mir-561 MI0003567 (SEQ ID NO: 1241) 
               
               
                 CUUCAUCCACCAGUCCUCCAGGAACAUCAAGGAUCUUAAACUUUGCCAGAGCUACAAAGGCAAAGUUUAAGAUCCUU 
               
               
                 GAAGUUCCUGGGGGAACCAU 
               
               
                   
               
               
                 &gt;hsa-mir-562 MI0003568 (SEQ ID NO: 1242) 
               
               
                 AGUGAAAUUGCUAGGUCAUAUGGUCAGUCUACUUUUAGAGUAAUUGUGAAACUGUUUUUCAAAGUAGCUGUACCAUU 
               
               
                 UGCACUCCCUGUGGCAAU 
               
               
                   
               
               
                 &gt;hsa-mir-563 MI0003569 (SEQ ID NO: 1243) 
               
               
                 AGCAAAGAAGUGUGUUGCCCUCUAGGAAAUGUGUGUUGCUCUGAUGUAAUUAGGUUGACAUACGUUUCCCUGGUAGC 
               
               
                 CA 
               
               
                   
               
               
                 &gt;hsa-mir-564 MI0003570 (SEQ ID NO: 1244) 
               
               
                 CGGGCAGCGGGUGCCAGGCACGGUGUCAGCAGGCAACAUGGCCGAGAGGCCGGGGCCUCCGGGCGGCGCCGUGUCCG 
               
               
                 CGACCGCGUACCCUGAC 
               
               
                   
               
               
                 &gt;hsa-mir-566 MI0003572 (SEQ ID NO: 1245) 
               
               
                 GCUAGGCGUGGUGGCGGGCGCCUGUGAUCCCAACUACUCAGGAGGCUGGGGCAGCAGAAUCGCUUGAACCCGGGAGG 
               
               
                 CGAAGGUUGCAGUGAGC 
               
               
                   
               
               
                 &gt;hsa-mir-567 MI0003573 (SEQ ID NO: 1246) 
               
               
                 GGAUUCUUAUAGGACAGUAUGUUCUUCCAGGACAGAACAUUCUUUGCUAUUUUGUACUGGAAGAACAUGCAAAACUA 
               
               
                 AAAAAAAAAAAAGUUAUUGCU 
               
               
                   
               
               
                 &gt;hsa-mir-568 MI0003574 (SEQ ID NO: 1247) 
               
               
                 GAUAUACACUAUAUUAUGUAUAAAUGUAUACACACUUCCUAUAUGUAUCCACAUAUAUAUAGUGUAUAUAUUAUACA 
               
               
                 UGUAUAGGUGUGUAUAUG 
               
               
                   
               
               
                 &gt;hsa-mir-569 MI0003576 (SEQ ID NO: 1248) 
               
               
                 GGUAUUGUUAGAUUAAUUUUGUGGGACAUUAACAACAGCAUCAGAAGCAACAUCAGCUUUAGUUAAUGAAUCCUGGA 
               
               
                 AAGUUAAGUGACUUUAUUU 
               
               
                   
               
               
                 &gt;hsa-mir-570 MI0003577 (SEQ ID NO: 1249) 
               
               
                 CUAGAUAAGUUAUUAGGUGGGUGCAAAGGUAAUUGCAGUUUUUCCCAUUAUUUUAAUUGCGAAAACAGCAAUUACCU 
               
               
                 UUGCACCAACCUGAUGGAGU 
               
               
                   
               
               
                 &gt;hsa-mir-571 MI0003578 (SEQ ID NO: 1250) 
               
               
                 CCUCAGUAAGACCAAGCUCAGUGUGCCAUUUCCUUGUCUGUAGCCAUGUCUAUGGGCUCUUGAGUUGGCCAUCUGAG 
               
               
                 UGAGGGCCUGCUUAUUCUA 
               
               
                   
               
               
                 &gt;hsa-mir-572 MI0003579 (SEQ ID NO: 1251) 
               
               
                 GUCGAGGCCGUGGCCCGGAAGUGGUCGGGGCCGCUGCGGGCGGAAGGGCGCCUGUGCUUCGUCCGCUCGGCGGUGGC 
               
               
                 CCAGCCAGGCCCGCGGGA 
               
               
                   
               
               
                 &gt;hsa-mir-573 MI0003580 (SEQ ID NO: 1252) 
               
               
                 UUUAGCGGUUUCUCCCUGAAGUGAUGUGUAACUGAUCAGGAUCUACUCAUGUCGUCUUUGGUAAAGUUAUGUCGCUU 
               
               
                 GUCAGGGUGAGGAGAGUUUUUG 
               
               
                   
               
               
                 &gt;hsa-mir-574 MI0003581 (SEQ ID NO: 1253) 
               
               
                 GGGACCUGCGUGGGUGCGGGCGUGUGAGUGUGUGUGUGUGAGUGUGUGUCGCUCCGGGUCCACGCUCAUGCACACAC 
               
               
                 CCACACGCCCACACUCAGG 
               
               
                   
               
               
                 &gt;hsa-mir-575 MI0003582 (SEQ ID NO: 1254) 
               
               
                 AAUUCAGCCCUGCCACUGGCUUAUGUCAUGACCUUGGGCUACUCAGGCUGUCUGCACAAUGAGCCAGUUGGACAGGA 
               
               
                 GCAGUGCCACUCAACUC 
               
               
                   
               
               
                 &gt;hsa-mir-576 MI0003583 (SEQ ID NO: 1255) 
               
               
                 UACAAUCCAACGAGGAUUCUAAUUUCUCCACGUCUUUGGUAAUAAGGUUUGGCAAAGAUGUGGAAAAAUUGGAAUCC 
               
               
                 UCAUUCGAUUGGUUAUAACCA 
               
               
                   
               
               
                 &gt;hsa-mir-577 MI0003584 (SEQ ID NO: 1256) 
               
               
                 UGGGGGAGUGAAGAGUAGAUAAAAUAUUGGUACCUGAUGAAUCUGAGGCCAGGUUUCAAUACUUUAUCUGCUCUUCA 
               
               
                 UUUCCCCAUAUCUACUUAC 
               
               
                   
               
               
                 &gt;hsa-mir-578 MI0003585 (SEQ ID NO: 1257) 
               
               
                 AGAUAAAUCUAUAGACAAAAUACAAUCCCGGACAACAAGAAGCUCCUAUAGCUCCUGUAGCUUCUUGUGCUCUAGGA 
               
               
                 UUGUAUUUUGUUUAUAUAU 
               
               
                   
               
               
                 &gt;hsa-mir-579 MI0003586 (SEQ ID NO: 1258) 
               
               
                 CAUAUUAGGUUAAUGCAAAAGUAAUCGCGGUUUGUGCCAGAUGACGAUUUGAAUUAAUAAAUUCAUUUGGUAUAAAC 
               
               
                 CGCGAUUAUUUUUGCAUCAAC 
               
               
                   
               
               
                 &gt;hsa-mir-580 MI0003587 (SEQ ID NO: 1259) 
               
               
                 AUAAAAUUUCCAAUUGGAACCUAAUGAUUCAUCAGACUCAGAUAUUUAAGUUAACAGUAUUUGAGAAUGAUGAAUCA 
               
               
                 UUAGGUUCCGGUCAGAAAUU 
               
               
                   
               
               
                 &gt;hsa-mir-581 MI0003588 (SEQ ID NO: 1260) 
               
               
                 GUUAUGUGAAGGUAUUCUUGUGUUCUCUAGAUCAGUGCUUUUAGAAAAUUUGUGUGAUCUAAAGAACACAAAGAAUA 
               
               
                 CCUACACAGAACCACCUGC 
               
               
                   
               
               
                 &gt;hsa-mir-582 MI0003589 (SEQ ID NO: 1261) 
               
               
                 AUCUGUGCUCUUUGAUUACAGUUGUUCAACCAGUUACUAAUCUAACUAAUUGUAACUGGUUGAACAACUGAACCCAA 
               
               
                 AGGGUGCAAAGUAGAAACAUU 
               
               
                   
               
               
                 &gt;hsa-mir-583 MI0003590 (SEQ ID NO: 1262) 
               
               
                 AACUCACACAUUAACCAAAGAGGAAGGUCCCAUUACUGCAGGGAUCUUAGCAGUACUGGGACCUACCUCUUUGGU 
               
               
                   
               
               
                 &gt;hsa-mir-584 MI0003591 (SEQ ID NO: 1263) 
               
               
                 UAGGGUGACCAGCCAUUAUGGUUUGCCUGGGACUGAGGAAUUUGCUGGGAUAUGUCAGUUCCAGGCCAACCAGGCUG 
               
               
                 GUUGGUCUCCCUGAAGCAAC 
               
               
                   
               
               
                 &gt;hsa-mir-585 MI0003592 (SEQ ID NO: 1264) 
               
               
                 UGGGGUGUCUGUGCUAUGGCAGCCCUAGCACACAGAUACGCCCAGAGAAAGCCUGAACGUUGGGCGUAUCUGUAUGC 
               
               
                 UAGGGCUGCUGUAACAA 
               
               
                   
               
               
                 &gt;hsa-mir-586 MI0003594 (SEQ ID NO: 1265) 
               
               
                 AUGGGGUAAAACCAUUAUGCAUUGUAUUUUUAGGUCCCAAUACAUGUGGGCCCUAAAAAUACAAUGCAUAAUGGUUU 
               
               
                 UUCACUCUUUAUCUUCUUAU 
               
               
                   
               
               
                 &gt;hsa-mir-587 MI0003595 (SEQ ID NO: 1266) 
               
               
                 CUCCUAUGCACCCUCUUUCCAUAGGUGAUGAGUCACAGGGCUCAGGGAAUGUGUCUGCACCUGUGACUCAUCACCAG 
               
               
                 UGGAAAGCCCAUCCCAUAU 
               
               
                   
               
               
                 &gt;hsa-mir-588 MI0003597 (SEQ ID NO: 1267) 
               
               
                 AGCUUAGGUACCAAUUUGGCCACAAUGGGUUAGAACACUAUUCCAUUGUGUUCUUACCCACCAUGGCCAAAAUUGGG 
               
               
                 CCUAAG 
               
               
                   
               
               
                 &gt;hsa-mir-589 MI0003599 (SEQ ID NO: 1268) 
               
               
                 UCCAGCCUGUGCCCAGCAGCCCCUGAGAACCACGUCUGCUCUGAGCUGGGUACUGCCUGUUCAGAACAAAUGCCGGU 
               
               
                 UCCCAGACGCUGCCAGCUGGCC 
               
               
                   
               
               
                 &gt;hsa-mir-590 MI0003602 (SEQ ID NO: 1269) 
               
               
                 UAGCCAGUCAGAAAUGAGCUUAUUCAUAAAAGUGCAGUAUGGUGAAGUCAAUCUGUAAUUUUAUGUAUAAGCUAGUC 
               
               
                 UCUGAUUGAAACAUGCAGCA 
               
               
                   
               
               
                 &gt;hsa-mir-591 MI0003603 (SEQ ID NO: 1270) 
               
               
                 UCUUAUCAAUGAGGUAGACCAUGGGUUCUCAUUGUAAUAGUGUAGAAUGUUGGUUAACUGUGGACUCCCUGGCUCUG 
               
               
                 UCUCAAAUCUACUGAUUC 
               
               
                   
               
               
                 &gt;hsa-mir-592 MI0003604 (SEQ ID NO: 1271) 
               
               
                 UAUUAUGCCAUGACAUUGUGUCAAUAUGCGAUGAUGUGUUGUGAUGGCACAGCGUCAUCACGUGGUGACGCAACAUC 
               
               
                 AUGACGUAAGACGUCACAAC 
               
               
                   
               
               
                 &gt;hsa-mir-593 MI0003605 (SEQ ID NO: 1272) 
               
               
                 CCCCCAGAAUCUGUCAGGCACCAGCCAGGCAUUGCUCAGCCCGUUUCCCUCUGGGGGAGCAAGGAGUGGUGCUGGGU 
               
               
                 UUGUCUCUGCUGGGGUUUCUCCU 
               
               
                   
               
               
                 &gt;hsa-mir-595 MI0003607 (SEQ ID NO: 1273) 
               
               
                 ACGGAAGCCUGCACGCAUUUAACACCAGCACGCUCAAUGUAGUCUUGUAAGGAACAGGUUGAAGUGUGCCGUGGUGU 
               
               
                 GUCUGGAGGAAGCGCCUGU 
               
               
                   
               
               
                 &gt;hsa-mir-596 MI0003608 (SEQ ID NO: 1274) 
               
               
                 AGCACGGCCUCUCCGAAGCCUGCCCGGCUCCUCGGGAACCUGCCUCCCGCAUGGCAGCUGCUGCCCUUCGGAGGCCG 
               
               
                   
               
               
                 &gt;hsa-mir-597 MI0003609 (SEQ ID NO: 1275) 
               
               
                 UACUUACUCUACGUGUGUGUCACUCGAUGACCACUGUGAAGACAGUAAAAUGUACAGUGGUUCUCUUGUGGCUCAAG 
               
               
                 CGUAAUGUAGAGUACUGGUC 
               
               
                   
               
               
                 &gt;hsa-mir-598 MI0003610 (SEQ ID NO: 1276) 
               
               
                 GCUUGAUGAUGCUGCUGAUGCUGGCGGUGAUCCCGAUGGUGUGAGCUGGAAAUGGGGUGCUACGUCAUCGUUGUCAU 
               
               
                 CGUCAUCAUCAUCAUCCGAG 
               
               
                   
               
               
                 &gt;hsa-mir-599 MI0003611 (SEQ ID NO: 1277) 
               
               
                 AAAGACAUGCUGUCCACAGUGUGUUUGAUAAGCUGACAUGGGACAGGGAUUCUUUUCACUGUUGUGUCAGUUUAUCA 
               
               
                 AACCCAUACUUGGAUGAC 
               
               
                   
               
               
                 &gt;hsa-mir-600 MI0003613 (SEQ ID NO: 1278) 
               
               
                 AAGUCACGUGCUGUGGCUCCAGCUUCAUAGGAAGGCUCUUGUCUGUCAGGCAGUGGAGUUACUUACAGACAAGAGCC 
               
               
                 UUGCUCAGGCCAGCCCUGCCC 
               
               
                   
               
               
                 &gt;hsa-mir-601 MI0003614 (SEQ ID NO: 1279) 
               
               
                 UGCAUGAGUUCGUCUUGGUCUAGGAUUGUUGGAGGAGUCAGAAAAACUACCCCAGGGAUCCUGAAGUCCUUUGGGUG 
               
               
                 GA 
               
               
                   
               
               
                 &gt;hsa-mir-602 MI0003615 (SEQ ID NO: 1280) 
               
               
                 UUCUCACCCCCGCCUGACACGGGCGACAGCUGCGGCCCGCUGUGUUCACUCGGGCCGAGUGCGUCUCCUGUCAGGCA 
               
               
                 AGGGAGAGCAGAGCCCCCCUG 
               
               
                   
               
               
                 &gt;hsa-mir-603 MI0003616 (SEQ ID NO: 1281) 
               
               
                 GAUUGAUGCUGUUGGUUUGGUGCAAAAGUAAUUGCAGUGCUUCCCAUUUAAAAGUAAUGGCACACACUGCAAUUACU 
               
               
                 UUUGCUCCAACUUAAUACUU 
               
               
                   
               
               
                 &gt;hsa-mir-604 MI0003617 (SEQ ID NO: 1282) 
               
               
                 AGAGCAUCGUGCUUGACCUUCCACGCUCUCGUGUCCACUAGCAGGCAGGUUUUCUGACACAGGCUGCGGAAUUCAGG 
               
               
                 ACAGUGCAUCAUGGAGA 
               
               
                   
               
               
                 &gt;hsa-mir-605 MI0003618 (SEQ ID NO: 1283) 
               
               
                 GCCCUAGCUUGGUUCUAAAUCCCAUGGUGCCUUCUCCUUGGGAAAAACAGAGAAGGCACUAUGAGAUUUAGAAUCAA 
               
               
                 GUUAGG 
               
               
                   
               
               
                 &gt;hsa-mir-606 MI0003619 (SEQ ID NO: 1284) 
               
               
                 UGUAUCCUUGGUUUUUAGUAGUUUUACUAUGAUGAGGUGUGCCAUCCACCCCAUCAUAGUAAACUACUGAAAAUCAA 
               
               
                 AGAUACAAGUGCCUGACCA 
               
               
                   
               
               
                 &gt;hsa-mir-607 MI0003620 (SEQ ID NO: 1285) 
               
               
                 UUGCCUAAAGUCACACAGGUUAUAGAUCUGGAUUGGAACCCAGGGAGCCAGACUGCCUGGGUUCAAAUCCAGAUCUA 
               
               
                 UAACUUGUGUGACUUUGGG 
               
               
                   
               
               
                 &gt;hsa-mir-608 MI0003621 (SEQ ID NO: 1286) 
               
               
                 GGGCCAAGGUGGGCCAGGGGUGGUGUUGGGACAGCUCCGUUUAAAAAGGCAUCUCCAAGAGCUUCCAUCAAAGGCUG 
               
               
                 CCUCUUGGUGCAGCACAGGUAGA 
               
               
                   
               
               
                 &gt;hsa-mir-609 MI0003622 (SEQ ID NO: 1287) 
               
               
                 UGCUCGGCUGUUCCUAGGGUGUUUCUCUCAUCUCUGGUCUAUAAUGGGUUAAAUAGUAGAGAUGAGGGCAACACCCU 
               
               
                 AGGAACAGCAGAGGAACC 
               
               
                   
               
               
                 &gt;hsa-mir-610 MI0003623 (SEQ ID NO: 1288) 
               
               
                 UCUAUUUGUCUUAGGUGAGCUAAAUGUGUGCUGGGACACAUUUGAGCCAAAUGUCCCAGCACACAUUUAGCUCACAU 
               
               
                 AAGAAAAAUGGACUCUAGU 
               
               
                   
               
               
                 &gt;hsa-mir-611 MI0003624 (SEQ ID NO: 1289) 
               
               
                 AAAAUGGUGAGAGCGUUGAGGGGAGUUCCAGACGGAGAUGCGAGGACCCCUCGGGGUCUGACCCACA 
               
               
                   
               
               
                 &gt;hsa-mir-612 MI0003625 (SEQ ID NO: 1290) 
               
               
                 UCCCAUCUGGACCCUGCUGGGCAGGGCUUCUGAGCUCCUUAGCACUAGCAGGAGGGGCUCCAGGGGCCCUCCCUCCA 
               
               
                 UGGCAGCCAGGACAGGACUCUCA 
               
               
                   
               
               
                 &gt;hsa-mir-613 MI0003626 (SEQ ID NO: 1291) 
               
               
                 GGUGAGUGCGUUUCCAAGUGUGAAGGGACCCUUCCUGUAGUGUCUUAUAUACAAUACAGUAGGAAUGUUCCUUCUUU 
               
               
                 GCCACUCAUACACCUUUA 
               
               
                   
               
               
                 &gt;hsa-mir-614 MI0003627 (SEQ ID NO: 1292) 
               
               
                 UCUAAGAAACGCAGUGGUCUCUGAAGCCUGCAGGGGCAGGCCAGCCCUGCACUGAACGCCUGUUCUUGCCAGGUGGC 
               
               
                 AGAAGGUUGCUGC 
               
               
                   
               
               
                 &gt;hsa-mir-615 MI0003628 (SEQ ID NO: 1293) 
               
               
                 CUCGGGAGGGGCGGGAGGGGGGUCCCCGGUGCUCGGAUCUCGAGGGUGCUUAUUGUUCGGUCCGAGCCUGGGUCUCC 
               
               
                 CUCUUCCCCCCAACCCCCC 
               
               
                   
               
               
                 &gt;hsa-mir-616 MI0003629 (SEQ ID NO: 1294) 
               
               
                 UUAGGUAAUUCCUCCACUCAAAACCCUUCAGUGACUUCCAUGACAUGAAAUAGGAAGUCAUUGGAGGGUUUGAGCAG 
               
               
                 AGGAAUGACCUGUUUUAAAA 
               
               
                   
               
               
                 &gt;hsa-mir-617 MI0003631 (SEQ ID NO: 1295) 
               
               
                 CAUCAUAAGGAGCCUAGACUUCCCAUUUGAAGGUGGCCAUUUCCUACCACCUUCAAAUGGUAAGUCCAGGCUCCUUC 
               
               
                 UGAUUCAAUAAAUGAGGAGC 
               
               
                   
               
               
                 &gt;hsa-mir-618 MI0003632 (SEQ ID NO: 1296) 
               
               
                 CUCUUGUUCACAGCCAAACUCUACUUGUCCUUCUGAGUGUAAUUACGUACAUGCAGUAGCUCAGGAGACAAGCAGGU 
               
               
                 UUACCCUGUGGAUGAGUCUGA 
               
               
                   
               
               
                 &gt;hsa-mir-619 MI0003633 (SEQ ID NO: 1297) 
               
               
                 CGCCCACCUCAGCCUCCCAAAAUGCUGGGAUUACAGGCAUGAGCCACUGCGGUCGACCAUGACCUGGACAUGUUUGU 
               
               
                 GCCCAGUACUGUCAGUUUGCAG 
               
               
                   
               
               
                 &gt;hsa-mir-620 MI0003634 (SEQ ID NO: 1298) 
               
               
                 AUAUAUAUCUAUAUCUAGCUCCGUAUAUAUAUAUAUAUAUAUAUAGAUAUCUCCAUAUAUAUGGAGAUAGAUAUAGA 
               
               
                 AAUAAAACAAGCAAAGAA 
               
               
                   
               
               
                 &gt;hsa-mir-621 MI0003635 (SEQ ID NO: 1299) 
               
               
                 UAGAUUGAGGAAGGGGCUGAGUGGUAGGCGGUGCUGCUGUGCUCUGAUGAAGACCCAUGUGGCUAGCAACAGCGCUU 
               
               
                 ACCUUUUGUCUCUGGGUCC 
               
               
                   
               
               
                 &gt;hsa-mir-622 MI0003636 (SEQ ID NO: 1300) 
               
               
                 AGAGAAGCUGGACAAGUACUGGUCUCAGCAGAUUGAGGAGAGCACCACAGUGGUCAUCACACAGUCUGCUGAGGUUG 
               
               
                 GAGCUGCUGAGAUGACACU 
               
               
                   
               
               
                 &gt;hsa-mir-623 MI0003637 (SEQ ID NO: 1301) 
               
               
                 GUACACAGUAGAAGCAUCCCUUGCAGGGGCUGUUGGGUUGCAUCCUAAGCUGUGCUGGAGCUUCCCGAUGUACUCUG 
               
               
                 UAGAUGUCUUUGCACCUUCUG 
               
               
                   
               
               
                 &gt;hsa-mir-624 MI0003638 (SEQ ID NO: 1302) 
               
               
                 AAUGCUGUUUCAAGGUAGUACCAGUACCUUGUGUUCAGUGGAACCAAGGUAAACACAAGGUAUUGGUAUUACCUUGA 
               
               
                 GAUAGCAUUACACCUAAGUG 
               
               
                   
               
               
                 &gt;hsa-mir-625 MI0003639 (SEQ ID NO: 1303) 
               
               
                 AGGGUAGAGGGAUGAGGGGGAAAGUUCUAUAGUCCUGUAAUUAGAUCUCAGGACUAUAGAACUUUCCCCCUCAUCCC 
               
               
                 UCUGCCCU 
               
               
                   
               
               
                 &gt;hsa-mir-626 MI0003640 (SEQ ID NO: 1304) 
               
               
                 ACUGAUAUAUUUGUCUUAUUUGAGAGCUGAGGAGUAUUUUUAUGCAAUCUGAAUGAUCUCAGCUGUCUGAAAAUGUC 
               
               
                 UUCAAUUUUAAAGGCUU 
               
               
                   
               
               
                 &gt;hsa-mir-627 MI0003641 (SEQ ID NO: 1305) 
               
               
                 UACUUAUUACUGGUAGUGAGUCUCUAAGAAAAGAGGAGGUGGUUGUUUUCCUCCUCUUUUCUUUGAGACUCACUACC 
               
               
                 AAUAAUAAGAAAUACUACUA 
               
               
                   
               
               
                 &gt;hsa-mir-628 MI0003642 (SEQ ID NO: 1306) 
               
               
                 AUAGCUGUUGUGUCACUUCCUCAUGCUGACAUAUUUACUAGAGGGUAAAAUUAAUAACCUUCUAGUAAGAGUGGCAG 
               
               
                 UCGAAGGGAAGGGCUCAU 
               
               
                   
               
               
                 &gt;hsa-mir-629 MI0003643 (SEQ ID NO: 1307) 
               
               
                 UCCCUUUCCCAGGGGAGGGGCUGGGUUUACGUUGGGAGAACUUUUACGGUGAACCAGGAGGUUCUCCCAACGUAAGC 
               
               
                 CCAGCCCCUCCCCUCUGCCU 
               
               
                   
               
               
                 &gt;hsa-mir-630 MI0003644 (SEQ ID NO: 1308) 
               
               
                 AACUUAACAUCAUGCUACCUCUUUGUAUCAUAUUUUGUUAUUCUGGUCACAGAAUGACCUAGUAUUCUGUACCAGGG 
               
               
                 AAGGUAGUUCUUAACUAUAU 
               
               
                   
               
               
                 &gt;hsa-mir-631 MI0003645 (SEQ ID NO: 1309) 
               
               
                 GUGGGGAGCCUGGUUAGACCUGGCCCAGACCUCAGCUACACAAGCUGAUGGACUGAGUCAGGGGCCACACUCUCC 
               
               
                   
               
               
                 &gt;hsa-mir-632 MI0003647 (SEQ ID NO: 1310) 
               
               
                 CGCCUCCUACCGCAGUGCUUGACGGGAGGCGGAGCGGGGAACGAGGCCGUCGGCCAUUUUGUGUCUGCUUCCUGUGG 
               
               
                 GACGUGGUGGUAGCCGU 
               
               
                   
               
               
                 &gt;hsa-mir-633 MI0003648 (SEQ ID NO: 1311) 
               
               
                 AACCUCUCUUAGCCUCUGUUUCUUUAUUGCGGUAGAUACUAUUAACCUAAAAUGAGAAGGCUAAUAGUAUCUACCAC 
               
               
                 AAUAAAAUUGUUGUGAGGAUA 
               
               
                   
               
               
                 &gt;hsa-mir-634 MI0003649 (SEQ ID NO: 1312) 
               
               
                 AAACCCACACCACUGCAUUUUGGCCAUCGAGGGUUGGGGCUUGGUGUCAUGCCCCAAGAUAACCAGCACCCCAACUU 
               
               
                 UGGACAGCAUGGAUUAGUCU 
               
               
                   
               
               
                 &gt;hsa-mir-635 MI0003650 (SEQ ID NO: 1313) 
               
               
                 CAGAGAGGAGCUGCCACUUGGGCACUGAAACAAUGUCCAUUAGGCUUUGUUAUGGAAACUUCUCCUGAUCAUUGUUU 
               
               
                 UGUGUCCAUUGAGCUUCCAAU 
               
               
                   
               
               
                 &gt;hsa-mir-636 MI0003651 (SEQ ID NO: 1314) 
               
               
                 UGGCGGCCUGGGCGGGAGCGCGCGGGCGGGGCCGGCCCCGCUGCCUGGAAUUAACCCCGCUGUGCUUGCUCGUCCCG 
               
               
                 CCCGCAGCCCUAGGCGGCGUCG 
               
               
                   
               
               
                 &gt;hsa-mir-637 MI0003652 (SEQ ID NO: 1315) 
               
               
                 UGGCUAAGGUGUUGGCUCGGGCUCCCCACUGCAGUUACCCUCCCCUCGGCGUUACUGAGCACUGGGGGCUUUCGGGC 
               
               
                 UCUGCGUCUGCACAGAUACUUC 
               
               
                   
               
               
                 &gt;hsa-mir-638 MI0003653 (SEQ ID NO: 1316) 
               
               
                 GUGAGCGGGCGCGGCAGGGAUCGCGGGCGGGUGGCGGCCUAGGGCGCGGAGGGCGGACCGGGAAUGGCGCGCCGUGC 
               
               
                 GCCGCCGGCGUAACUGCGGCGCU 
               
               
                   
               
               
                 &gt;hsa-mir-639 MI0003654 (SEQ ID NO: 1317) 
               
               
                 UGGCCGACGGGGCGCGCGCGGCCUGGAGGGGCGGGGCGGACGCAGAGCCGCGUUUAGUCUAUCGCUGCGGUUGCGAG 
               
               
                 CGCUGUAGGGAGCCUGUGCUG 
               
               
                   
               
               
                 &gt;hsa-mir-640 MI0003655 (SEQ ID NO: 1318) 
               
               
                 GUGACCCUGGGCAAGUUCCUGAAGAUCAGACACAUCAGAUCCCUUAUCUGUAAAAUGGGCAUGAUCCAGGAACCUGC 
               
               
                 CUCUACGGUUGCCUUGGGG 
               
               
                   
               
               
                 &gt;hsa-mir-641 MI0003656 (SEQ ID NO: 1319) 
               
               
                 UGGGUGAAAGGAAGGAAAGACAUAGGAUAGAGUCACCUCUGUCCUCUGUCCUCUACCUAUAGAGGUGACUGUCCUAU 
               
               
                 GUCUUUCCUUCCUCUUACCCCU 
               
               
                   
               
               
                 &gt;hsa-mir-642a MI0003657 (SEQ ID NO: 1320) 
               
               
                 AUCUGAGUUGGGAGGGUCCCUCUCCAAAUGUGUCUUGGGGUGGGGGAUCAAGACACAUUUGGAGAGGGAACCUCCCA 
               
               
                 ACUCGGCCUCUGCCAUCAUU 
               
               
                   
               
               
                 &gt;hsa-mir-642b MI0016685 (SEQ ID NO: 1321) 
               
               
                 GAGUUGGGAGGUUCCCUCUCCAAAUGUGUCUUGAUCCCCCACCCCAAGACACAUUUGGAGAGGGACCCUCCCAACUC 
               
               
                   
               
               
                 &gt;hsa-mir-643 MI0003658 (SEQ ID NO: 1322) 
               
               
                 ACCAAGUGAUAUUCAUUGUCUACCUGAGCUAGAAUACAAGUAGUUGGCGUCUUCAGAGACACUUGUAUGCUAGCUCA 
               
               
                 GGUAGAUAUUGAAUGAAAAA 
               
               
                   
               
               
                 &gt;hsa-mir-644a MI0003659 (SEQ ID NO: 1323) 
               
               
                 UUUUUUUUUAGUAUUUUUCCAUCAGUGUUCAUAAGGAAUGUUGCUCUGUAGUUUUCUUAUAGUGUGGCUUUCUUAGA 
               
               
                 GCAAAGAUGGUUCCCUA 
               
               
                   
               
               
                 &gt;hsa-mir-644b MI0019134 (SEQ ID NO: 1324) 
               
               
                 UGGGCUAAGGGAGAUGAUUGGGUAGAAAGUAUUAUUCUAUUCAUUUGCCUCCCAGCCUACA 
               
               
                   
               
               
                 &gt;hsa-mir-645 MI0003660 (SEQ ID NO: 1325) 
               
               
                 CAGUUCCUAACAGGCCUCAGACCAGUACCGGUCUGUGGCCUGGGGGUUGAGGACCCCUGCUCUAGGCUGGUACUGCU 
               
               
                 GAUGCUUAAAAAGAGAG 
               
               
                   
               
               
                 &gt;hsa-mir-646 MI0003661 (SEQ ID NO: 1326) 
               
               
                 GAUCAGGAGUCUGCCAGUGGAGUCAGCACACCUGCUUUUCACCUGUGAUCCCAGGAGAGGAAGCAGCUGCCUCUGAG 
               
               
                 GCCUCAGGCUCAGUGGC 
               
               
                   
               
               
                 &gt;hsa-mir-647 MI0003662 (SEQ ID NO: 1327) 
               
               
                 AGGAAGUGUUGGCCUGUGGCUGCACUCACUUCCUUCAGCCCCAGGAAGCCUUGGUCGGGGGCAGGAGGGAGGGUCAG 
               
               
                 GCAGGGCUGGGGGCCUGAC 
               
               
                   
               
               
                 &gt;hsa-mir-648 MI0003663 (SEQ ID NO: 1328) 
               
               
                 AUCACAGACACCUCCAAGUGUGCAGGGCACUGGUGGGGGCCGGGGCAGGCCCAGCGAAAGUGCAGGACCUGGCACUU 
               
               
                 AGUCGGAAGUGAGGGUG 
               
               
                   
               
               
                 &gt;hsa-mir-649 MI0003664 (SEQ ID NO: 1329) 
               
               
                 GGCCUAGCCAAAUACUGUAUUUUUGAUCGACAUUUGGUUGAAAAAUAUCUAUGUAUUAGUAAACCUGUGUUGUUCAA 
               
               
                 GAGUCCACUGUGUUUUGCUG 
               
               
                   
               
               
                 &gt;hsa-mir-650 MI0003665 (SEQ ID NO: 1330) 
               
               
                 CAGUGCUGGGGUCUCAGGAGGCAGCGCUCUCAGGACGUCACCACCAUGGCCUGGGCUCUGCUCCUCCUCACCCUCCU 
               
               
                 CACUCAGGGCACAGGUGAU 
               
               
                   
               
               
                 &gt;hsa-mir-651 MI0003666 (SEQ ID NO: 1331) 
               
               
                 AAUCUAUCACUGCUUUUUAGGAUAAGCUUGACUUUUGUUCAAAUAAAAAUGCAAAAGGAAAGUGUAUCCUAAAAGGC 
               
               
                 AAUGACAGUUUAAUGUGUUU 
               
               
                   
               
               
                 &gt;hsa-mir-652 MI0003667 (SEQ ID NO: 1332) 
               
               
                 ACGAAUGGCUAUGCACUGCACAACCCUAGGAGAGGGUGCCAUUCACAUAGACUAUAAUUGAAUGGCGCCACUAGGGU 
               
               
                 UGUGCAGUGCACAACCUACAC 
               
               
                   
               
               
                 &gt;hsa-mir-653 MI0003674 (SEQ ID NO: 1333) 
               
               
                 UUCAUUCCUUCAGUGUUGAAACAAUCUCUACUGAACCAGCUUCAAACAAGUUCACUGGAGUUUGUUUCAAUAUUGCA 
               
               
                 AGAAUGAUAAGAUGGAAGC 
               
               
                   
               
               
                 &gt;hsa-mir-654 MI0003676 (SEQ ID NO: 1334) 
               
               
                 GGGUAAGUGGAAAGAUGGUGGGCCGCAGAACAUGUGCUGAGUUCGUGCCAUAUGUCUGCUGACCAUCACCUUUAGAA 
               
               
                 GCCC 
               
               
                   
               
               
                 &gt;hsa-mir-655 MI0003677 (SEQ ID NO: 1335) 
               
               
                 AACUAUGCAAGGAUAUUUGAGGAGAGGUUAUCCGUGUUAUGUUCGCUUCAUUCAUCAUGAAUAAUACAUGGUUAACC 
               
               
                 UCUUUUUGAAUAUCAGACUC 
               
               
                   
               
               
                 &gt;hsa-mir-656 MI0003678 (SEQ ID NO: 1336) 
               
               
                 CUGAAAUAGGUUGCCUGUGAGGUGUUCACUUUCUAUAUGAUGAAUAUUAUACAGUCAACCUCUUUCCGAUAUCGAAUC 
               
               
                   
               
               
                 &gt;hsa-mir-657 MI0003681 (SEQ ID NO: 1337) 
               
               
                 GUGUAGUAGAGCUAGGAGGAGAGGGUCCUGGAGAAGCGUGGACCGGUCCGGGUGGGUUCCGGCAGGUUCUCACCCUC 
               
               
                 UCUAGGCCCCAUUCUCCUCUG 
               
               
                   
               
               
                 &gt;hsa-mir-658 MI0003682 (SEQ ID NO: 1338) 
               
               
                 GCUCGGUUGCCGUGGUUGCGGGCCCUGCCCGCCCGCCAGCUCGCUGACAGCACGACUCAGGGCGGAGGGAAGUAGGU 
               
               
                 CCGUUGGUCGGUCGGGAACGAGG 
               
               
                   
               
               
                 &gt;hsa-mir-659 MI0003683 (SEQ ID NO: 1339) 
               
               
                 UACCGACCCUCGAUUUGGUUCAGGACCUUCCCUGAACCAAGGAAGAGUCACAGUCUCUUCCUUGGUUCAGGGAGGGU 
               
               
                 CCCCAACAAUGUCCUCAUGG 
               
               
                   
               
               
                 &gt;hsa-mir-660 MI0003684 (SEQ ID NO: 1340) 
               
               
                 CUGCUCCUUCUCCCAUACCCAUUGCAUAUCGGAGUUGUGAAUUCUCAAAACACCUCCUGUGUGCAUGGAUUACAGGA 
               
               
                 GGGUGAGCCUUGUCAUCGUG 
               
               
                   
               
               
                 &gt;hsa-mir-661 MI0003669 (SEQ ID NO: 1341) 
               
               
                 GGAGAGGCUGUGCUGUGGGGCAGGCGCAGGCCUGAGCCCUGGUUUCGGGCUGCCUGGGUCUCUGGCCUGCGCGUGAC 
               
               
                 UUUGGGGUGGCU 
               
               
                   
               
               
                 &gt;hsa-mir-662 MI0003670 (SEQ ID NO: 1342) 
               
               
                 GCUGUUGAGGCUGCGCAGCCAGGCCCUGACGGUGGGGUGGCUGCGGGCCUUCUGAAGGUCUCCCACGUUGUGGCCCA 
               
               
                 GCAGCGCAGUCACGUUGC 
               
               
                   
               
               
                 &gt;hsa-mir-663a MI0003672 (SEQ ID NO: 1343) 
               
               
                 CCUUCCGGCGUCCCAGGCGGGGCGCCGCGGGACCGCCCUCGUGUCUGUGGCGGUGGGAUCCCGCGGCCGUGUUUUCC 
               
               
                 UGGUGGCCCGGCCAUG 
               
               
                   
               
               
                 &gt;hsa-mir-663b MI0006336 (SEQ ID NO: 1344) 
               
               
                 GGUGCCGAGGGCCGUCCGGCAUCCUAGGCGGGUCGCUGCGGUACCUCCCUCCUGUCUGUGGCGGUGGGAUCCCGUGG 
               
               
                 CCGUGUUUUCCUGGUGGCCCGGCCGUGCCUGAGGUUUC 
               
               
                   
               
               
                 &gt;hsa-mir-664 MI0006442 (SEQ ID NO: 1345) 
               
               
                 GAACAUUGAAACUGGCUAGGGAAAAUGAUUGGAUAGAAACUAUUAUUCUAUUCAUUUAUCCCCAGCCUACAAAAUGA 
               
               
                 AAAAA 
               
               
                   
               
               
                 &gt;hsa-mir-665 MI0005563 (SEQ ID NO: 1346) 
               
               
                 UCUCCUCGAGGGGUCUCUGCCUCUACCCAGGACUCUUUCAUGACCAGGAGGCUGAGGCCCCUCACAGGCGGC 
               
               
                   
               
               
                 &gt;hsa-mir-668 MI0003761 (SEQ ID NO: 1347) 
               
               
                 GGUAAGUGCGCCUCGGGUGAGCAUGCACUUAAUGUGGGUGUAUGUCACUCGGCUCGGCCCACUACC 
               
               
                   
               
               
                 &gt;hsa-mir-670 MI0003933 (SEQ ID NO: 1348) 
               
               
                 GUUUAGGGGUGGACCUGAUGUCCCUGAGUGUAUGUGGUGAACCUGAAUUUGCCUUGGGUUUCCUCAUAUUCAUUCAG 
               
               
                 GAGUGUCAGUUGCCCCUUCAC 
               
               
                   
               
               
                 &gt;hsa-mir-671 MI0003760 (SEQ ID NO: 1349) 
               
               
                 GCAGGUGAACUGGCAGGCCAGGAAGAGGAGGAAGCCCUGGAGGGGCUGGAGGUGAUGGAUGUUUUCCUCCGGUUCUC 
               
               
                 AGGGCUCCACCUCUUUCGGGCCGUAGAGCCAGGGCUGGUGC 
               
               
                   
               
               
                 &gt;hsa-mir-675 MI0005416 (SEQ ID NO: 1350) 
               
               
                 CCCAGGGUCUGGUGCGGAGAGGGCCCACAGUGGACUUGGUGACGCUGUAUGCCCUCACCGCUCAGCCCCUGGG 
               
               
                   
               
               
                 &gt;hsa-mir-676 MI0016436 (SEQ ID NO: 1351) 
               
               
                 GCAUGACUCUUCAACCUCAGGACUUGCAGAAUUAAUGGAAUGCUGUCCUAAGGUUGUUGAGUUGUGC 
               
               
                   
               
               
                 &gt;hsa-mir-708 MI0005543 (SEQ ID NO: 1352) 
               
               
                 AACUGCCCUCAAGGAGCUUACAAUCUAGCUGGGGGUAAAUGACUUGCACAUGAACACAACUAGACUGUGAGCUUCUA 
               
               
                 GAGGGCAGGGA 
               
               
                   
               
               
                 &gt;hsa-mir-711 MI0012488 (SEQ ID NO: 1353) 
               
               
                 ACUGACUUUGAGUCUCUCCUCAGGGUGCUGCAGGCAAAGCUGGGGACCCAGGGAGAGACGUAAGUGAGGGGAGAUG 
               
               
                   
               
               
                 &gt;hsa-mir-718 MI0012489 (SEQ ID NO: 1354) 
               
               
                 GGCCGCGGCGCGCAAGAUGGCGGCGGGCCCGGGCACCGCCCCUUCCGCCCCGCCGGGCGUCGCACGAGGC 
               
               
                   
               
               
                 &gt;hsa-mir-720 MI0006654 (SEQ ID NO: 1355) 
               
               
                 CCGGAUCUCACACGGUGGUGUUAAUAUCUCGCUGGGGCCUCCAAAAUGUUGUGCCCAGGGGUGUUAGAGAAAACACC 
               
               
                 ACACUUUGAGAUGAAUUAAGAGUCCUUUAUUAG 
               
               
                   
               
               
                 &gt;hsa-mir-744 MI0005559 (SEQ ID NO: 1356) 
               
               
                 UUGGGCAAGGUGCGGGGCUAGGGCUAACAGCAGUCUUACUGAAGGUUUCCUGGAAACCACGCACAUGCUGUUGCCAC 
               
               
                 UAACCUCAACCUUACUCGGUC 
               
               
                   
               
               
                 &gt;hsa-mir-758 MI0003757 (SEQ ID NO: 1357) 
               
               
                 GCCUGGAUACAUGAGAUGGUUGACCAGAGAGCACACGCUUUAUUUGUGCCGUUUGUGACCUGGUCCACUAACCCUCA 
               
               
                 GUAUCUAAUGC 
               
               
                   
               
               
                 &gt;hsa-mir-759 MI0004065 (SEQ ID NO: 1358) 
               
               
                 UAAUAAAUUAAAUGCCUAAACUGGCAGAGUGCAAACAAUUUUGACUCAGAUCUAAAUGUUUGCACUGGCUGUUUAAA 
               
               
                 CAUUUAAUUUGUUA 
               
               
                   
               
               
                 &gt;hsa-mir-760 MI0005567 (SEQ ID NO: 1359) 
               
               
                 GGCGCGUCGCCCCCCUCAGUCCACCAGAGCCCGGAUACCUCAGAAAUUCGGCUCUGGGUCUGUGGGGAGCGAAAUGC 
               
               
                 AAC 
               
               
                   
               
               
                 &gt;hsa-mir-761 MI0003941 (SEQ ID NO: 1360) 
               
               
                 GGAGGAGCAGCAGGGUGAAACUGACACAGUUCUGGUGAGUUUCACUUUGCUGCUCCUCC 
               
               
                   
               
               
                 &gt;hsa-mir-762 MI0003892 (SEQ ID NO: 1361) 
               
               
                 GGCCCGGCUCCGGGUCUCGGCCCGUACAGUCCGGCCGGCCAUGCUGGCGGGGCUGGGGCCGGGGCCGAGCCCGCGGC 
               
               
                 GGGGCC 
               
               
                   
               
               
                 &gt;hsa-mir-764 MI0003944 (SEQ ID NO: 1362) 
               
               
                 AAUCUAGGAGGCAGGUGCUCACUUGUCCUCCUCCAUGCUUGGAAAAUGCAGGGAGGAGGCCAUAGUGGCAACUGUUA 
               
               
                 CCAUGAUU 
               
               
                   
               
               
                 &gt;hsa-mir-765 MI0005116 (SEQ ID NO: 1363) 
               
               
                 UUUAGGCGCUGAUGAAAGUGGAGUUCAGUAGACAGCCCUUUUCAAGCCCUACGAGAAACUGGGGUUUCUGGAGGAGA 
               
               
                 AGGAAGGUGAUGAAGGAUCUGUUCUCGUGAGCCUGAA 
               
               
                   
               
               
                 &gt;hsa-mir-766 MI0003836 (SEQ ID NO: 1364) 
               
               
                 GCAUCCUCAGGACCUGGGCUUGGGUGGUAGGAGGAAUUGGUGCUGGUCUUUCAUUUUGGAUUUGACUCCAGCCCCAC 
               
               
                 AGCCUCAGCCACCCCAGCCAAUUGUCAUAGGAGC 
               
               
                   
               
               
                 &gt;hsa-mir-767 MI0003763 (SEQ ID NO: 1365) 
               
               
                 GCUUUUAUAUUGUAGGUUUUUGCUCAUGCACCAUGGUUGUCUGAGCAUGCAGCAUGCUUGUCUGCUCAUACCCCAUG 
               
               
                 GUUUCUGAGCAGGAACCUUCAUUGUCUACUGC 
               
               
                   
               
               
                 &gt;hsa-mir-769 MI0003834 (SEQ ID NO: 1366) 
               
               
                 GCCUUGGUGCUGAUUCCUGGGCUCUGACCUGAGACCUCUGGGUUCUGAGCUGUGAUGUUGCUCUCGAGCUGGGAUCU 
               
               
                 CCGGGGUCUUGGUUCAGGGCCGGGGCCUCUGGGUUCCAAGC 
               
               
                   
               
               
                 &gt;hsa-mir-770 MI0005118 (SEQ ID NO: 1367) 
               
               
                 AGGAGCCACCUUCCGAGCCUCCAGUACCACGUGUCAGGGCCACAUGAGCUGGGCCUCGUGGGCCUGAUGUGGUGCUG 
               
               
                 GGGCCUCAGGGGUCUGCUCUU 
               
               
                   
               
               
                 &gt;hsa-mir-802 MI0003906 (SEQ ID NO: 1368) 
               
               
                 GUUCUGUUAUUUGCAGUCAGUAACAAAGAUUCAUCCUUGUGUCCAUCAUGCAACAAGGAGAAUCUUUGUCACUUAGU 
               
               
                 GUAAUUAAUAGCUGGAC 
               
               
                   
               
               
                 &gt;hsa-mir-873 MI0005564 (SEQ ID NO: 1369) 
               
               
                 GUGUGCAUUUGCAGGAACUUGUGAGUCUCCUAUUGAAAAUGAACAGGAGACUGAUGAGUUCCCGGGAACACCCACAA 
               
               
                   
               
               
                 &gt;hsa-mir-874 MI0005532 (SEQ ID NO: 1370) 
               
               
                 UUAGCCCUGCGGCCCCACGCACCAGGGUAAGAGAGACUCUCGCUUCCUGCCCUGGCCCGAGGGACCGACUGGCUGGGC 
               
               
                   
               
               
                 &gt;hsa-mir-875 MI0005541 (SEQ ID NO: 1371) 
               
               
                 UUAGUGGUACUAUACCUCAGUUUUAUCAGGUGUUCUUAAAAUCACCUGGAAACACUGAGGUUGUGUCUCACUGAAC 
               
               
                   
               
               
                 &gt;hsa-mir-876 MI0005542 (SEQ ID NO: 1372) 
               
               
                 UGAAGUGCUGUGGAUUUCUUUGUGAAUCACCAUAUCUAAGCUAAUGUGGUGGUGGUUUACAAAGUAAUUCAUAGUGC 
               
               
                 UUCA 
               
               
                   
               
               
                 &gt;hsa-mir-877 MI0005561 (SEQ ID NO: 1373) 
               
               
                 GUAGAGGAGAUGGCGCAGGGGACACGGGCAAAGACUUGGGGGUUCCUGGGACCCUCAGACGUGUGUCCUCUUCUCCC 
               
               
                 UCCUCCCAG 
               
               
                   
               
               
                 &gt;hsa-mir-885 MI0005560 (SEQ ID NO: 1374) 
               
               
                 CCGCACUCUCUCCAUUACACUACCCUGCCUCUUCUCCAUGAGAGGCAGCGGGGUGUAGUGGAUAGAGCACGGGU 
               
               
                   
               
               
                 &gt;hsa-mir-887 MI0005562 (SEQ ID NO: 1375) 
               
               
                 GUGCAGAUCCUUGGGAGCCCUGUUAGACUCUGGAUUUUACACUUGGAGUGAACGGGCGCCAUCCCGAGGCUUUGCAC 
               
               
                 AG 
               
               
                   
               
               
                 &gt;hsa-mir-888 MI0005537 (SEQ ID NO: 1376) 
               
               
                 GGCAGUGCUCUACUCAAAAAGCUGUCAGUCACUUAGAUUACAUGUGACUGACACCUCUUUGGGUGAAGGAAGGCUCA 
               
               
                   
               
               
                 &gt;hsa-mir-889 MI0005540 (SEQ ID NO: 1377) 
               
               
                 GUGCUUAAAGAAUGGCUGUCCGUAGUAUGGUCUCUAUAUUUAUGAUGAUUAAUAUCGGACAACCAUUGUUUUAGUAU 
               
               
                 CC 
               
               
                   
               
               
                 &gt;hsa-mir-890 MI0005533 (SEQ ID NO: 1378) 
               
               
                 GGAAGUGCCCUACUUGGAAAGGCAUCAGUUGCUUAGAUUACAUGUAACUAUUCCCUUUCUGAGUAGAGUAAGUCUUA 
               
               
                   
               
               
                 &gt;hsa-mir-891a MI0005524 (SEQ ID NO: 1379) 
               
               
                 CCUUAAUCCUUGCAACGAACCUGAGCCACUGAUUCAGUAAAAUACUCAGUGGCACAUGUUUGUUGUGAGGGUCAAAA 
               
               
                 GA 
               
               
                   
               
               
                 &gt;hsa-mir-891b MI0005534 (SEQ ID NO: 1380) 
               
               
                 CCUUAAUCCUUGCAACUUACCUGAGUCAUUGAUUCAGUAAAACAUUCAAUGGCACAUGUUUGUUGUUAGGGUCAAAA 
               
               
                 GA 
               
               
                   
               
               
                 &gt;hsa-mir-892a MI0005528 (SEQ ID NO: 1381) 
               
               
                 GCAGUGCCUUACUCAGAAAGGUGCCAGUCACUUACACUACAUGUCACUGUGUCCUUUCUGCGUAGAGUAAGGCUC 
               
               
                   
               
               
                 &gt;hsa-mir-892b MI0005538 (SEQ ID NO: 1382) 
               
               
                 UGCAAUGCCCUACUCAGAAAGGUGCCAUUUAUGUAGAUUUUAUGUCACUGGCUCCUUUCUGGGUAGAGCAAGGCUCA 
               
               
                   
               
               
                 &gt;hsa-mir-920 MI0005712 (SEQ ID NO: 1383) 
               
               
                 GUAGUUGUUCUACAGAAGACCUGGAUGUGUAGGAGCUAAGACACACUCCAGGGGAGCUGUGGAAGCAGUAACACG 
               
               
                   
               
               
                 &gt;hsa-mir-921 MI0005713 (SEQ ID NO: 1384) 
               
               
                 ACUAGUGAGGGACAGAACCAGGAUUCAGACUCAGGUCCAUGGGCCUGGAUCACUGG 
               
               
                   
               
               
                 &gt;hsa-mir-922 MI0005714 (SEQ ID NO: 1385) 
               
               
                 AUGGCGUUUUCCCUCUCCCUGUCCUGGACUGGGGUCAGACUGUGCCCCGAGGAGAAGCAGCAGAGAAUAGGACUACG 
               
               
                 UCAU 
               
               
                   
               
               
                 &gt;hsa-mir-924 MI0005716 (SEQ ID NO: 1386) 
               
               
                 AAUAGAGUCUUGUGAUGUCUUGCUUAAGGGCCAUCCAACCUAGAGUCUACAAC 
               
               
                   
               
               
                 &gt;hsa-mir-933 MI0005755 (SEQ ID NO: 1387) 
               
               
                 ACUUGGGUCAGUUCAGAGGUCCUCGGGGCGCGCGUCGAGUCAGCCGUGUGCGCAGGGAGACCUCUCCCACCCACAGU 
               
               
                   
               
               
                 &gt;hsa-mir-934 MI0005756 (SEQ ID NO: 1388) 
               
               
                 AGAAAUAAGGCUUCUGUCUACUACUGGAGACACUGGUAGUAUAAAACCCAGAGUCUCCAGUAAUGGACGGGAGCCUU 
               
               
                 AUUUCU 
               
               
                   
               
               
                 &gt;hsa-mir-935 MI0005757 (SEQ ID NO: 1389) 
               
               
                 GGCGGGGGCGCGGGCGGCAGUGGCGGGAGCGGCCCCUCGGCCAUCCUCCGUCUGCCCAGUUACCGCUUCCGCUACCG 
               
               
                 CCGCCGCUCCCGCU 
               
               
                   
               
               
                 &gt;hsa-mir-936 MI0005758 (SEQ ID NO: 1390) 
               
               
                 UCAAGGCCACUGGGACAGUAGAGGGAGGAAUCGCAGAAAUCACUCCAGGAGCAACUGAGAGACCUUGCUUCUACUUU 
               
               
                 ACCAGGUCCUGCUGGCCCAGA 
               
               
                   
               
               
                 &gt;hsa-mir-937 MI0005759 (SEQ ID NO: 1391) 
               
               
                 AGCACUGCCCCCGGUGAGUCAGGGUGGGGCUGGCCCCCUGCUUCGUGCCCAUCCGCGCUCUGACUCUCUGCCCACCU 
               
               
                 GCAGGAGCU 
               
               
                   
               
               
                 &gt;hsa-mir-938 MI0005760 (SEQ ID NO: 1392) 
               
               
                 GAAGGUGUACCAUGUGCCCUUAAAGGUGAACCCAGUGCACCUUCAUGAACCGUGGUACACCUUUAAGAACUUGGUAU 
               
               
                 GCCUUC 
               
               
                   
               
               
                 &gt;hsa-mir-939 MI0005761 (SEQ ID NO: 1393) 
               
               
                 UGUGGGCAGGGCCCUGGGGAGCUGAGGCUCUGGGGGUGGCCGGGGCUGACCCUGGGCCUCUGCUCCCCAGUGUCUGA 
               
               
                 CCGCG 
               
               
                   
               
               
                 &gt;hsa-mir-940 MI0005762 (SEQ ID NO: 1394) 
               
               
                 GUGAGGUGUGGGCCCGGCCCCAGGAGCGGGGCCUGGGCAGCCCCGUGUGUUGAGGAAGGAAGGCAGGGCCCCCGCUC 
               
               
                 CCCGGGCCUGACCCCAC 
               
               
                   
               
               
                 &gt;hsa-mir-941-1 MI0005763 (SEQ ID NO: 1395) 
               
               
                 CACGGAAGAGGACACACCCGGCUGUGUGGACAUGUGCCCAGGGCCCGGGACAGCGCCACGGAAGAGGACGCACCCGG 
               
               
                 CUGUGUGCACAUGUGCCCAGGGCCCGGGACAGCGCCACGG 
               
               
                   
               
               
                 &gt;hsa-mir-941-2 MI0005764 (SEQ ID NO: 1396) 
               
               
                 CACGGAAGAGGACGCACCCGGCUGUGUGCACAUGUGCCCAGGGCCCGGGACAGCGCCACGGAAGAGGACGCACCCGG 
               
               
                 CUGUGUGCACAUGUGCCCAGGGCCCGGGACAGCGCCACGG 
               
               
                   
               
               
                 &gt;hsa-mir-941-3 MI0005765 (SEQ ID NO: 1397) 
               
               
                 CACGGAAGAGGACGCACCCGGCUGUGUGCACAUGUGCCCAGGGCCCGGGACAGCGCCACGGAAGAGGACGCACCCGG 
               
               
                 CUGUGUGCACAUGUGCCCAGGGCCCGGGACAGCGCCAUGG 
               
               
                   
               
               
                 &gt;hsa-mir-941-4 MI0005766 (SEQ ID NO: 1398) 
               
               
                 CAUGGAAGAGGACGCACCCGGCUGUGUGCACAUGUGCCCAGGGCCCGGGACAGCGCCACGGAAGAGGACGCACCCGG 
               
               
                 CUGUGUGCACAUGUGCCCAGGGCCCGGGACAGCGCCACGG 
               
               
                   
               
               
                 &gt;hsa-mir-942 MI0005767 (SEQ ID NO: 1399) 
               
               
                 AUUAGGAGAGUAUCUUCUCUGUUUUGGCCAUGUGUGUACUCACAGCCCCUCACACAUGGCCGAAACAGAGAAGUUAC 
               
               
                 UUUCCUAAU 
               
               
                   
               
               
                 &gt;hsa-mir-943 MI0005768 (SEQ ID NO: 1400) 
               
               
                 GGGACGUUCUGAGCUCGGGGUGGGGGACGUUUGCCGGUCACUGCUGCUGGCGCCCUGACUGUUGCCGUCCUCCAGCC 
               
               
                 CCACUCAAAGGCAUCCC 
               
               
                   
               
               
                 &gt;hsa-mir-944 MI0005769 (SEQ ID NO: 1401) 
               
               
                 GUUCCAGACACAUCUCAUCUGAUAUACAAUAUUUUCUUAAAUUGUAUAAAGAGAAAUUAUUGUACAUCGGAUGAGCU 
               
               
                 GUGUCUGGGAU 
               
               
                   
               
               
                 &gt;hsa-mir-1178 MI0006271 (SEQ ID NO: 1402) 
               
               
                 GCGUUGGCUGGCAGAGGAAGGGAAGGGUCCAGGGUCAGCUGAGCAUGCCCUCAGGUUGCUCACUGUUCUUCCCUAGA 
               
               
                 AUGUCAGGUGAUGU 
               
               
                   
               
               
                 &gt;hsa-mir-1179 MI0006272 (SEQ ID NO: 1403) 
               
            
           
           
               
               
            
               
                 GGCUGGAAAGGAAGAAGCAUUCUUUCAUUGGUUGGUGUGUAUUGCCUUGUCAACCAAUAAGAGGAUGCCAUUUAUCC 
                   
               
               
                 UUUUCUGACUAGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1180 MI0006273 (SEQ ID NO: 1404) 
                   
               
               
                 GCUGCUGGACCCACCCGGCCGGGAAUAGUGCUCCUGGUUGUUUCCGGCUCGCGUGGGUGUGUCGGCGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1181 MI0006274 (SEQ ID NO: 1405) 
                   
               
               
                 UCCACUGCUGCCGCCGUCGCCGCCACCCGAGCCGGAGCGGGCUGGGCCGCCAAGGCAAGAUGGUGGACUACAGCGUG 
                   
               
               
                 UGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1182 MI0006275 (SEQ ID NO: 1406) 
                   
               
               
                 GGGACUUGUCACUGCCUGUCUCCUCCCUCUCCAGCAGCGACUGGAUUCUGGAGUCCAUCUAGAGGGUCUUGGGAGGG 
                   
               
               
                 AUGUGACUGUUGGGAAGCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1183 MI0006276 (SEQ ID NO: 1407) 
                   
               
               
                 AUUAUUCAAAUGCUCGGAGACACAGAACAUUAGAGAAGACAGGAGUUCACUGUAGGUGAUGGUGAGAGUGGGCAUGG 
                   
               
               
                 AGCAGGAGUGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1184-1 MI0006277 (SEQ ID NO: 1408) 
                   
               
               
                 CUUGCAGAACGAGGUGAAGGAGGUGGUUCUGCUCAGCAGUCAACAGUGGCCACAUCUCCACCUGCAGCGACUUGAUG 
                   
               
               
                 GCUUCCGUGUCCUUUUCGUGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1184-2 MI0015971 (SEQ ID NO: 1409) 
                   
               
               
                 CUUGCAGAACGAGGUGAAGGAGGUGGUUCUGCUCAGCAGUCAACAGUGGCCACAUCUCCACCUGCAGCGACUUGAUG 
                   
               
               
                 GCUUCCGUGUCCUUUUCGUGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1184-3 MI0015972 (SEQ ID NO: 1410) 
                   
               
               
                 CUUGCAGAACGAGGUGAAGGAGGUGGUUCUGCUCAGCAGUCAACAGUGGCCACAUCUCCACCUGCAGCGACUUGAUG 
                   
               
               
                 GCUUCCGUGUCCUUUUCGUGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1185-l MI0003844 (SEQ ID NO: 1411) 
                   
               
               
                 UUUGGUACUUGAAGAGAGGAUACCCUUUGUAUGUUCACUUGAUUAAUGGCGAAUAUACAGGGGGAGACUCUUAUUUG 
                   
               
               
                 CGUAUCAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1185-2 MI0003821 (SEQ ID NO: 1412) 
                   
               
               
                 UUUGGUACUUAAAGAGAGGAUACCCUUUGUAUGUUCACUUGAUUAAUGGCGAAUAUACAGGGGGAGACUCUCAUUUG 
                   
               
               
                 CGUAUCAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1193 MI0014205 (SEQ ID NO: 1413) 
                   
               
               
                 GUAGCUGAGGGGAUGGUAGACCGGUGACGUGCACUUCAUUUACGAUGUAGGUCACCCGUUUGACUAUCCACCAGCGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1197 MI0006656 (SEQ ID NO: 1414) 
                   
               
               
                 ACUUCCUGGUAUUUGAAGAUGCGGUUGACCAUGGUGUGUACGCUUUAUUUGUGACGUAGGACACAUGGUCUACUUCU 
                   
               
               
                 UCUCAAUAUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1200 MI0006332 (SEQ ID NO: 1415) 
                   
               
               
                 UGCUACUUCUCCUGAGCCAUUCUGAGCCUCAAUCACUUGCCAGAGAGAUUGGUUCAGGAAUUUGUCAGGGAUAGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1202 MI0006334 (SEQ ID NO: 1416) 
                   
               
               
                 CCUGCUGCAGAGGUGCCAGCUGCAGUGGGGGAGGCACUGCCAGGGCUGCCCACUCUGCUUAGCCAGCAGGUGCCAAG 
                   
               
               
                 AACAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1203 MI0006335 (SEQ ID NO: 1417) 
                   
               
               
                 UCCUCCCCGGAGCCAGGAUGCAGCUCAAGCCACAGCAGGGUGUUUAGCGCUCUUCAGUGGCUCCAGAUUGUGGCGCU 
                   
               
               
                 GGUGCAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1204 MI0006337 (SEQ ID NO: 1418) 
                   
               
               
                 ACCUCGUGGCCUGGUCUCCAUUAUUUGAGAUGAGUUACAUCUUGGAGGUGAGGACGUGCCUCGUGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1205 MI0006338 (SEQ ID NO: 1419) 
                   
               
               
                 GAAGGCCUCUGCAGGGUUUGCUUUGAGGUACUUCCUUCCUGUCAACCCUGUUCUGGAGUCUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1206 MI0006339 (SEQ ID NO: 1420) 
                   
               
               
                 CAGUGUUCAUGUAGAUGUUUAAGCUCUUGCAGUAGGUUUUUGCAAGCUAGUGAACGCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1207 MI0006340 (SEQ ID NO: 1421) 
                   
               
               
                   
               
               
                 GCAGGGCUGGCAGGGAGGCUGGGAGGGGCUGGCUGGGUCUGGUAGUGGGCAUCAGCUGGCCCUCAUUUCUUAAGACA 
                   
               
               
                 GCACUUCUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1208 MI0006341 (SEQ ID NO: 1422) 
                   
               
               
                 CACCGGCAGAAUCACUGUUCAGACAGGCGGAGACGGGUCUUUCUCGCCCUCUGAUGAGUCACCACUGUGGUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1224 MI0003764 (SEQ ID NO: 1423) 
                   
               
               
                 GUGAGGACUCGGGAGGUGGAGGGUGGUGCCGCCGGGGCCGGGCGCUGUUUCAGCUCGCUUCUCCCCCCACCUCCUCU 
                   
               
               
                 CUCCUCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1225 MI0006311 (SEQ ID NO: 1424) 
                   
               
               
                 GUGGGUACGGCCCAGUGGGGGGGAGAGGGACACGCCCUGGGCUCUGCCCAGGGUGCAGCCGGACUGACUGAGCCCCU 
                   
               
               
                 GUGCCGCCCCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1226 MI0006313 (SEQ ID NO: 1425) 
                   
               
               
                 GUGAGGGCAUGCAGGCCUGGAUGGGGCAGCUGGGAUGGUCCAAAAGGGUGGCCUCACCAGCCCUGUGUUCCCUAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1227 MI0006316 (SEQ ID NO: 1426) 
                   
               
               
                 GUGGGGCCAGGCGGUGGUGGGCACUGCUGGGGUGGGCACAGCAGCCAUGCAGAGCGGGCAUUUGACCCCGUGCCACC 
                   
               
               
                 CUUUUCCCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1228 MI0006318 (SEQ ID NO: 1427) 
                   
               
               
                 GUGGGCGGGGGCAGGUGUGUGGUGGGUGGUGGCCUGCGGUGAGCAGGGCCCUCACACCUGCCUCGCCCCCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1229 MI0006319 (SEQ ID NO: 1428) 
                   
               
               
                 GUGGGUAGGGUUUGGGGGAGAGCGUGGGCUGGGGUUCAGGGACACCCUCUCACCACUGCCCUCCCACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1231 MI0006321 (SEQ ID NO: 1429) 
                   
               
               
                 GUCAGUGUCUGGGCGGACAGCUGCAGGAAAGGGAAGACCAAGGCUUGCUGUCUGUCCAGUCUGCCACCCUACCCUGU 
                   
               
               
                 CUGUUCUUGCCACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1233-1 MI0006323 (SEQ ID NO: 1430) 
                   
               
               
                 GUGAGUGGGAGGCCAGGGCACGGCAGGGGGAGCUGCAGGGCUAUGGGAGGGGCCCCAGCGUCUGAGCCCUGUCCUCC 
                   
               
               
                 CGCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1233-2 MI0015973 (SEQ ID NO: 1431) 
                   
               
               
                 GUGAGUGGGAGGCCAGGGCACGGCAGGGGGAGCUGCAGGGCUAUGGGAGGGGCCCCAGCGUCUGAGCCCUGUCCUCC 
                   
               
               
                 CGCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1234 MI0006324 (SEQ ID NO: 1432) 
                   
               
               
                 GUGAGUGUGGGGUGGCUGGGGGGGGGGGGGGGGGGCCGGGGACGGCUUGGGCCUGCCUAGUCGGCCUGACCACCCAC 
                   
               
               
                 CCCACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1236 MI0006326 (SEQ ID NO: 1433) 
                   
               
               
                 GUGAGUGACAGGGGAAAUGGGGAUGGACUGGAAGUGGGCAGCAUGGAGCUGACCUUCAUCAUGGCUUGGCCAACAUA 
                   
               
               
                 AUGCCUCUUCCCCUUGUCUCUCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1237 MI0006327 (SEQ ID NO: 1434) 
                   
               
               
                 GUGGGAGGGCCCAGGCGCGGGCAGGGGUGGGGGUGGCAGAGCGCUGUCCCGGGGGCGGGGCCGAAGCGCGGCGACCG 
                   
               
               
                 UAACUCCUUCUGCUCCGUCCCCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1238 MI0006328 (SEQ ID NO: 1435) 
                   
               
               
                 GUGAGUGGGAGCCCCAGUGUGUGGUUGGGGCCAUGGCGGGUGGGCAGCCCAGCCUCUGAGCCUUCCUCGUCUGUCUG 
                   
               
               
                 CCCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1243 MI0006373 (SEQ ID NO: 1436) 
                   
               
               
                 CUAAAACUGGAUCAAUUAUAGGAGUGAAAUAAAGGUCCAUCUCCUGCCUAUUUAUUACUUUGCUUUGGUAAUAAAUC 
                   
               
               
                 UAUUUUUAAAAGAACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1244-1 MI0006379 (SEQ ID NO: 1437) 
                   
               
               
                 AUCUUAUUCCGAGCAUUCCAGUAACUUUUUUGUGUAUGUACUUAGCUGUACUAUAAGUAGUUGGUUUGUAUGAGAUG 
                   
               
               
                 GUUAAAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1244-2 MI0015974 (SEQ ID NO: 1438) 
                   
               
               
                 AUCUUAUUCCGAGCAUUCCAGUAACUUUUUUGUGUAUGUACUUAGCUGUACUAUAAGUAGUUGGUUUGUAUGAGAUG 
                   
               
               
                 GUUAAAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1244-3 MI0015975 (SEQ ID NO: 1439) 
                   
               
               
                 AUCUUAUUCCGAGCAUUCCAGUAACUUUUUUGUGUAUGUACUUAGCUGUACUAUAAGUAGUUGGUUUGUAUGAGAUG 
                   
               
               
                 GUUAAAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1245a MI0006380 (SEQ ID NO: 1440) 
                   
               
               
                 AUUUAUGUAUAGGCCUUUAGAUCAUCUGAUGUUGAAUACUCUUUAAGUGAUCUAAAGGCCUACAUAUAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1245b MI0017431 (SEQ ID NO: 1441) 
                   
               
               
                 UUUAUAUGUAGGCCUUUAGAUCACUUAAAGAGUAUUCAACAUCAGAUGAUCUAAAGGCCUAUACAUAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1246 MI0006381 (SEQ ID NO: 1442) 
                   
               
               
                 UGUAUCCUUGAAUGGAUUUUUGGAGCAGGAGUGGACACCUGACCCAAAGGAAAUCAAUCCAUAGGCUAGCAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1247 MI0006382 (SEQ ID NO: 1443) 
                   
               
               
                 CCGCUUGCCUCGCCCAGCGCAGCCCCGGCCGCUGGGCGCACCCGUCCCGUUCGUCCCCGGACGUUGCUCUCUACCCC 
                   
               
               
                 GGGAACGUCGAGACUGGAGCGCCCGAACUGAGCCACCUUCGCGGACCCCGAGAGCGGCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1248 MI0006383 (SEQ ID NO: 1444) 
                   
               
               
                 UUUACCUUCUUGUAUAAGCACUGUGCUAAAAUUGCAGACACUAGGACCAUGUCUUGGUUUUUGCAAUAAUGCUAGCA 
                   
               
               
                 GAGUACACACAAGAAGAAAAGUAACAGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1249 MI0006384 (SEQ ID NO: 1445) 
                   
               
               
                 GGGAGGAGGGAGGAGAUGGGCCAAGUUCCCUCUGGCUGGAACGCCCUUCCCCCCCUUCUUCACCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1250 MI0006385 (SEQ ID NO: 1446) 
                   
               
               
                 CUGUCCCGCUGGCCUGGCAGGUGACGGUGCUGGAUGUGGCCUUUUUGCCUUUUCUAAAGGCCACAUUUUCCAGCCCA 
                   
               
               
                 UUCAACCUUCCAGAGCCCUCUGAAGUGGCCACAGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1251 MI0006386 (SEQ ID NO: 1447) 
                   
               
               
                 GUGGACUCUAGCUGCCAAAGGCGCUUCUCCUUCUGAACAGAGCGCUUUGCUCAGCCAGUGUAGACAUGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1252 MI0006434 (SEQ ID NO: 1448) 
                   
               
               
                 AGAAAGAAGGAAAUUGAAUUCAUUUAGAAAAGAGAAUUCCAAAUGAGCUUAAUUUCCUUUUUUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1253 MI0006387 (SEQ ID NO: 1449) 
                   
               
               
                 AGCAGCAAGAGAUAGAAUCCAAAAGAGAAGAAGAUCAGCCUGCAGAUGUGGACUGCUAAAUGCAGGCUGAUCUUCUC 
                   
               
               
                 CCCUUUGGGAUUCUCUUAUGAGAAGCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1254-1 MI0006388 (SEQ ID NO: 1450) 
                   
               
               
                 GGUGGGAGGAUUGCUUGAGCCUGGAAGCUGGAGCCUGCAGUGAACUAUCAUUGUGCCACUGUACUCCAGCCUAGGCA 
                   
               
               
                 ACAAAAUGAAAUCCUGUCUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1254-2 MI0016747 (SEQ ID NO: 1451) 
                   
               
               
                 CUGAGCCUGGAAGCUGGAGCCUGCAGUGAGCUAUGAUCAUGUCCCUGUACUCUAGCCUGGGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1255a MI0006389 (SEQ ID NO: 14523) 
                   
               
               
                 AUUGGAAAUCCUUUGAGUUGCUUCUCAAGGAUGAGCAAAGAAAGUAGAUUUUUUAGAUUCUAAAGAAACUAUCUUCU 
                   
               
               
                 UUGCUCAUCCUUGAGAAGCAACUCCUUAUCCAUUAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1255b-1 MI0006435 (SEQ ID NO: 1453) 
                   
               
               
                 UACGGAUGAGCAAAGAAAGUGGUUUCUUAAAAUGGAAUCUACUCUUUGUGAAGAUGCUGUGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1255b-2 MI0006436 (SEQ ID NO: 1454) 
                   
               
               
                 UCUUACGGAUGAGCAAAGAAAGUGGUUUGCGCCUCAAGAAACCACUUUCUUUGCUCAUCCAUAAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1256 MI0006390 (SEQ ID NO: 1455) 
                   
               
               
                 AGUCAGCCUGUUGAAGCUUUGAAGCUUUGAUGCCAGGCAUUGACUUCUCACUAGCUGUGAAAGUCCUAGCUAAAGAG 
                   
               
               
                 AAGUCAAUGCAUGACAUCUUGUUUCAAUAGAUGGCUGUUUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1257 MI0006391 (SEQ ID NO: 1456) 
                   
               
               
                 GCCCUGGGCUUGUGCUUGGGGAGUGAAUGAUGGGUUCUGACCCCCAUGCACCCCUGUGGGCCCCUGGCAUCACUGGC 
                   
               
               
                 CCCAUCCUUCACCCCUGCCAACCACGCUUGCCCUGUGCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1258 MI0006392 (SEQ ID NO: 1457) 
                   
               
               
                 CUGUGGCUUCCACGACCUAAUCCUAACUCCUGCGAGUCCCUGGAGUUAGGAUUAGGUCGUGGAAGCCACAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1260a MI0006394 (SEQ ID NO: 1458) 
                   
               
               
                 ACCUUUCCAGCUCAUCCCACCUCUGCCACCAAAACACUCAUCGCGGGGUCAGAGGGAGUGCCAAAAAAGGUAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1260b MI0014197 (SEQ ID NO: 1459) 
                   
               
               
                 UCUCCGUUUAUCCCACCACUGCCACCAUUAUUGCUACUGUUCAGCAGGUGCUGCUGGUGGUGAUGGUGAUAGUCUGG 
                   
               
               
                 UGGGGGCGGUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1261 MI0006396 (SEQ ID NO: 1460) 
                   
               
               
                 UGCUAUGGAUAAGGCUUUGGCUUAUGGGGAUAUUGUGGUUGAUCUGUUCUAUCCAGAUGACUGAAACUUUCUCCAUA 
                   
               
               
                 GCAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1262 MI0006397 (SEQ ID NO: 1461) 
                   
               
               
                 AUCUACAAUGGUGAUGGGUGAAUUUGUAGAAGGAUGAAAGUCAAAGAAUCCUUCUGGGAACUAAUUUUUGGCCUUCA 
                   
               
               
                 ACAAGAAUUGUGAUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1263 MI0006398 (SEQ ID NO: 1462) 
                   
               
               
                 CUACCCCAAAAUAUGGUACCCUGGCAUACUGAGUAUUUUAAUACUGGCAUACUCAGUAUGCCAUGUUGCCAUAUUUU 
                   
               
               
                 GGGGUAGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1264 MI0003758 (SEQ ID NO: 1463) 
                   
               
               
                 AGGUCCUCAAUAAGUAUUUGUUGAAAGAAUAAAUAAACCAACAAGUCUUAUUUGAGCACCUGUUAUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1265 MI0006401 (SEQ ID NO: 1464) 
                   
               
               
                 AUGGUUUGGGACUCAGGAUGUGGUCAAGUGUUGUUAAGGCAUGUUCAGGAACAAUACUUGACCACAUUUUGAAUUCC 
                   
               
               
                 AAACCAUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1266 MI0006403 (SEQ ID NO: 1465) 
                   
               
               
                 ACAGGUAGUGUCCCUCAGGGCUGUAGAACAGGGCUGGGAUUACUAAAGCCCUGUUCUAUGCCCUGAGGGACACUGAG 
                   
               
               
                 CAUGUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1267 MI0006404 (SEQ ID NO: 1466) 
                   
               
               
                 CUCCCAAAUCUCCUGUUGAAGUGUAAUCCCCACCUCCAGCAUUGGGGAUUACAUUUCAACAUGAGAUUUGGAUGAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1268a MI0006405 (SEQ ID NO: 1467) 
                   
               
               
                 UAGCCGGGCGUGGUGGUGGGGGCCUGUGGUCCCAGCUACUUUGGAGGCUGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1268b MI0016748 (SEQ ID NO: 1468) 
                   
               
               
                 ACCCGGGCGUGGUGGUGGGGGUGGGUGCCUGUAAUUCCAGCUAGUUGGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1269a MI0006406 (SEQ ID NO: 1469) 
                   
               
               
                 UGGAUUGCCUAGACCAGGGAAGCCAGUUGGCAUGGCUCAGUCCAAGUCUGACCACCUGAGGAAUGCCUGGACUGAGC 
                   
               
               
                 CGUGCUACUGGCUUCCCUGGUCUCCAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1269b MI0016888 (SEQ ID NO: 1470) 
                   
               
               
                 UGAGGUUUCUGGACUGAGCCAUGCUACUGGCUUCUCUGGUUCUCCAGCUUACAGAUGGCUUAUCAUGGGACCUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1270-1 MI0006407 (SEQ ID NO: 1471) 
                   
               
               
                 CACAGAGUUAUACUGGAGAUAUGGAAGAGCUGUGUUGGGUAUAAGUAACAGGCUUUUCUUUAUCUUCUAUGUGGCUC 
                   
               
               
                 UUUGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1270-2 MI0015976 (SEQ ID NO: 1472) 
                   
               
               
                 CACAGAGUUAUACUGGAGAUAUGGAAGAGCUGUGUUGGGUAUAAGUAACAGGCUUUUCUUUAUCUUCUAUGUGGCUC 
                   
               
               
                 UUUGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1271 MI0003814 (SEQ ID NO: 1473) 
                   
               
               
                 CACCCAGAUCAGUGCUUGGCACCUAGCAAGCACUCAGUAAAUAUUUGUUGAGUGCCUGCUAUGUGCCAGGCAUUGUG 
                   
               
               
                 CUGAGGGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1272 MI0006408 (SEQ ID NO: 1474) 
                   
               
               
                 CCAGAUCAGAUCUGGGUGCGAUGAUGAUGGCAGCAAAUUCUGAAAACGUGCUCAGUGUCUUUAUAACAGGAAAGCCG 
                   
               
               
                 UAAACUUAGAAAUGUAGGCUGCAGCUCGUGUGCUCUGUGGUCUGGGCUGGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1273a MI0006409 (SEQ ID NO: 1475) 
                   
               
               
                 UGAGGCAGGAGAAUUGCUUGAACCCGGGUGGUGGAGGUUGCAGUGAGCCAAGAUUGCGCCACUGCACUCCAGCCUGG 
                   
               
               
                 GCGACAAAGCAAGACUCUUUCUUGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1273c MI0014171 (SEQ ID NO: 1476) 
                   
               
               
                 UGCAGCCUGGGCGACAAAACGAGACCCUGUCUUUUUUUUUUUCUGAGACAGAGUCUCGUUCUGUUGCCCAAGCUGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1273d MI0014254 (SEQ ID NO: 1477) 
                   
               
               
                 GAAUCGCUUGAACCCAUGAGGUUGAGGCUGCAGUGAGCCAAGAUCGUGCCACUGCACUUCAGCCUGGGUGACAAGAG 
                   
               
               
                 CGAAACUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1273e MI0016059 (SEQ ID NO: 1478) 
                   
               
               
                 UGAGGCAGGAGAAUUGCUUGAACCCAGGAAGUGGAGGCUGCAGUGAGCCGAGAUCGAGCCACUGUACUCCAGCCUGG 
                   
               
               
                 GUGACACAGCGAGACUCCAGUCUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1273f MI0018002 (SEQ ID NO: 1479) 
                   
               
               
                 AGGUGGGAGGAUUGCUUGAGCCUGGGAGAUGGAGGUUGCAGUGAGCUGAGAUCACGCAACUGCACCCCCAGCCUGGG 
                   
               
               
                 CCAUAGAGUCAGUCCUUGUCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1273g MI0018003 (SEQ ID NO: 1480) 
                   
               
               
                 GAGGUGGGAGGAUUGCUUGAGUCAGGGUGGUUGAGGCUGCAGUAAGUUGUGAUCAUACCACUGCACUCCAGCCUGAG 
                   
               
               
                 UGACAGAGCAAGACCUUGUCUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1275 MI0006415 (SEQ ID NO: 1481) 
                   
               
               
                 CCUCUGUGAGAAAGGGUGUGGGGGAGAGGCUGUCUUGUGUCUGUAAGUAUGCCAAACUUAUUUUCCCCAAGGCAGAG 
                   
               
               
                 GGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1276 MI0006416 (SEQ ID NO: 1482) 
                   
               
               
                 CCCCAGCUAGGUAAAGAGCCCUGUGGAGACACCUGGAUUCAGAGAACAUGUCUCCACUGAGCACUUGGGCCUUGAUG 
                   
               
               
                 GCGGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1277 MI0006419 (SEQ ID NO: 1483) 
                   
               
               
                 ACCUCCCAAAUAUAUAUAUAUAUGUACGUAUGUGUAUAUAAAUGUAUACGUAGAUAUAUAUGUAUUUUUGGUGGGUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1278 MI0006425 (SEQ ID NO: 1484) 
                   
               
               
                 AUUUGCUCAUAGAUGAUAUGCAUAGUACUCCCAGAACUCAUUAAGUUGGUAGUACUGUGCAUAUCAUCUAUGAGCGA 
                   
               
               
                 AUAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1279 MI0006426 (SEQ ID NO: 1485) 
                   
               
               
                 AUAUUCACAAAAAUUCAUAUUGCUUCUUUCUAAUGCCAAGAAAGAAGAGUAUAAGAACUUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1280 MI0006437 (SEQ ID NO: 1486) 
                   
               
               
                 UCUGUCCCACCGCUGCCACCCUCCCCUCUGCCUCAGUGUGCCAGGCAUCAGCACUCACUCACAGAGGCAGGCUGGAU 
                   
               
               
                 GGCGGGUGGGACAACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1281 MI0006428 (SEQ ID NO: 1487) 
                   
               
               
                 AGGGGGCACCGGGAGGAGGUGAGUGUCUCUUGUCGCCUCCUCCUCUCCCCCCUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1282 MI0006429 (SEQ ID NO: 1488) 
                   
               
               
                 CCUUCUUCUCGUUUGCCUUUUUCUGCUUCUGCUGCAUGAUCUCCGAGUCCCUGGGGGUAGAGAUGAUGGGGCACUGG 
                   
               
               
                 GAGGUACCAGAGGGCAAAAAGGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1283-1 MI0003832 (SEQ ID NO: 1489) 
                   
               
               
                 CUCAAGCUAUGAGUCUACAAAGGAAAGCGCUUUCUGUUGUCAGAAAGAAGAGAAAGCGCUUCCCUUUUGAGGGUUAC 
                   
               
               
                 GGUUUGAGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1283-2 MI0006430 (SEQ ID NO: 1490) 
                   
               
               
                 CUCAAGCUGUGAGUCUACAAAGGAAAGCGCUUUCUGUUGUCUGAAAGAAAAGAAAUCGCUUCCCUUUGGAGUGUUAC 
                   
               
               
                 GGUUUGAGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1284 MI0006431 (SEQ ID NO: 1491) 
                   
               
               
                 AUUUUGAUAUAUAAGCCAGUUUAAUGUUUUCUAUACAGACCCUGGCUUUUCUUAAAUUUUAUAUAUUGGAAAGCCCA 
                   
               
               
                 UGUUUGUAUUGGAAACUGCUGGUUUCUUUCAUACUGAAAAUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1285-1 MI0006346 (SEQ ID NO: 1492) 
                   
               
               
                 UGUAGAGAUAGGAUCUCACUUUGUUGCCCAGGCUGGUCUCAAACUCCUGGUCUGGGCAACAAAGUGAGACCUUAUCU 
                   
               
               
                 CUACAAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1285-2 MI0006347 (SEQ ID NO: 1493) 
                   
               
               
                 UUUGGGAGGCCGAGGCUGGUGCAUCACUUGAGCCCAGCAAUUUGAGACCAAUCUGGGCAACAAAGUGAGACCUCCGU 
                   
               
               
                 CUCUACAAAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1286 MI0006348 (SEQ ID NO: 1494) 
                   
               
               
                 UGUCCUCUGGGGACUCAGCUUGCUCUGGCUGCUGGAUUGAAUUAGCUGCAGGACCAAGAUGAGCCCUUGGUGGAGACA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1287 MI0006349 (SEQ ID NO: 1495) 
                   
               
               
                 GUUGUGCUGUCCAGGUGCUGGAUCAGUGGUUCGAGUCUGAGCCUUUAAAAGCCACUCUAGCCACAGAUGCAGUGAUU 
                   
               
               
                 GGAGCCAUGACAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1288 MI0006432 (SEQ ID NO: 1496) 
                   
               
               
                 GAGGGUGUUGAUCAGCAGAUCAGGACUGUAACUCACCAUAGUGGUGGACUGCCCUGAUCUGGAGACCACUGCCUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1289-1 MI0006350 (SEQ ID NO: 1497) 
                   
               
               
                 UUCUCAAUUUUUAGUAGGAAUUAAAAACAAAACUGGUAAAUGCAGACUCUUGGUUUCCACCCCCAGAGAAUCCCUAA 
                   
               
               
                 ACCGGGGGUGGAGUCCAGGAAUCUGCAUUUUAGAAAGUACCCAGGGUGAUUCUGAUAAUUGGGAACA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1289-2 MI0006351 (SEQ ID NO: 1498) 
                   
               
               
                 CCACGGUCCUAGUUAAAAAGGCACAUUCCUAGACCCUGCCUCAGAACUACUGAACAGAGUCACUGGGUGUGGAGUCC 
                   
               
               
                 AGGAAUCUGCAUUUUUACCCCUAUCGCCCCCGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1290 MI0006352 (SEQ ID NO: 1499) 
                   
               
               
                 GAGCGUCACGUUGACACUCAAAAAGUUUCAGAUUUUGGAACAUUUCGGAUUUUGGAUUUUUGGAUCAGGGAUGCUCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1291 MI0006353 (SEQ ID NO: 1500) 
                   
               
               
                 GGUAGAAUUCCAGUGGCCCUGACUGAAGACCAGCAGUUGUACUGUGGCUGUUGGUUUCAAGCAGAGGCCUAAAGGAC 
                   
               
               
                 UGUCUUCCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1292 MI0006433 (SEQ ID NO: 1501) 
                   
               
               
                 CCUGGGAACGGGUUCCGGCAGACGCUGAGGUUGCGUUGACGCUCGCGCCCCGGCUCCCGUUCCAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1293 MI0006355 (SEQ ID NO: 1502) 
                   
               
               
                 AGGUUGUUCUGGGUGGUCUGGAGAUUUGUGCAGCUUGUACCUGCACAAAUCUCCGGACCACUUAGUCUUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1294 MI0006356 (SEQ ID NO: 1503) 
                   
               
               
                 CACCUAAUGUGUGCCAAGAUCUGUUCAUUUAUGAUCUCACCGAGUCCUGUGAGGUUGGCAUUGUUGUCUGGCAUUGU 
                   
               
               
                 CUGAUAUACAACAGUGCCAACCUCACAGGACUCAGUGAGGUGAAACUGAGGAUUAGGAAGGUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1295a MI0006357 (SEQ ID NO: 1504) 
                   
               
               
                 AGGACAUUUUGCCCAGAUCCGUGGCCUAUUCAGAAAUGUGGCCUGUGAUUAGGCCGCAGAUCUGGGUGAAAUGUCCU 
                   
               
               
                 CC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1295b MI0019146 (SEQ ID NO: 1505) 
                   
               
               
                 CACCCAGAUCUGCGGCCUAAUCACAGGCCACAUUUCUGAAUAGGCCACGGAUCUGGGCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1296 MI0003780 (SEQ ID NO: 1506) 
                   
               
               
                 ACCUACCUAACUGGGUUAGGGCCCUGGCUCCAUCUCCUUUAGGAAAACCUUCUGUGGGGAGUGGGGCUUCGACCCUA 
                   
               
               
                 ACCCAGGUGGGCUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1297 MI0006358 (SEQ ID NO: 1507) 
                   
               
               
                 UGUUUAUCUCUAGGGUUGAUCUAUUAGAAUUACUUAUCUGAGCCAAAGUAAUUCAAGUAAUUCAGGUGUAGUGAAAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1298 MI0003938 (SEQ ID NO: 1508) 
                   
               
               
                 AGACGAGGAGUUAAGAGUUCAUUCGGCUGUCCAGAUGUAUCCAAGUACCCUGUGUUAUUUGGCAAUAAAUACAUCUG 
                   
               
               
                 GGCAACUGACUGAACUUUUCACUUUUCAUGACUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1299 MI0006359 (SEQ ID NO: 1509) 
                   
               
               
                 CCUCAUGGCAGUGUUCUGGAAUCCUACGUGAGGGACAAUCAUUCAGACCCACGUAGCAGUGUUCUGGAAUUCUGUGU 
                   
               
               
                 GAGGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1301 MI0003815 (SEQ ID NO: 1510) 
                   
               
               
                 GGAUUGUGGGGGGUCGCUCUAGGCACCGCAGCACUGUGCUGGGGAUGUUGCAGCUGCCUGGGAGUGACUUCACACAG 
                   
               
               
                 UCCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-1 MI0006362 (SEQ ID NO: 1511) 
                   
               
               
                 CAGAAAGCCCAGUUAAAUUUGAAUUUCAAGUAAACAAUGAAUAAUUGUGUAUGUAAGAAUAUCCCAUACAAUAUUUG 
                   
               
               
                 GGACAUACUUAUGCUAAAAAUUAUUCCUUGCUUAUCUGAAAUUCAAAUGUAACUAGGAUUCCUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-10 MI0015979 (SEQ ID NO: 1512) 
                   
               
               
                 GGAUGCCCAGCUAGUUUGAAUUUUAGAUAAACAACGAAUAAUUUCGUAGCAUAAAUAUGUCCCAAGCUUAGUUUGGG 
                   
               
               
                 ACAUACUUAUGCUAAAAAACAUUAUUGGUUGUUUAUCUGAGAUUCAGAAUUAAGCAUUUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-11 MI0015980 (SEQ ID NO: 1513) 
                   
               
               
                 GGAUGCCCAGCUAGUUUGAAUUUUAGAUAAACAACGAAUAAUUUCGUAGCAUAAAUAUGUCCCAAGCUUAGUUUGGG 
                   
               
               
                 ACAUACUUAUGCUAAAAAACAUUAUUGGUUGUUUAUCUGAGAUUCAGAAUUAAGCAUUUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-2 MI0006363 (SEQ ID NO: 1514) 
                   
               
               
                 GGAUGCCCAGCUAGUUUGAAUUUUAGAUAAACAACGAAUAAUUUCGUAGCAUAAAUAUGUCCCAAGCUUAGUUUGGG 
                   
               
               
                 ACAUACUUAUGCUAAAAAACAUUAUUGGUUGUUUAUCUGAGAUUCAGAAUUAAGCAUUUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-3 MI0006364 (SEQ ID NO: 1515) 
                   
               
               
                 GGAUGCCCAGCUAGUUUGAAUUUUAGAUAAACAACGAAUAAUUUCGUAGCAUAAAUAUUUCCCAAGCUUAGUUUGGG 
                   
               
               
                 ACAUACUUAUGCUAAAAAACAUUAUUGGUUGUUUAUCUGAGAUUCAAAAUUAAGCAUUUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-4 MI0006365 (SEQ ID NO: 1516) 
                   
               
               
                 AAUGCAGAAGCACAGCUAAAAUUUGAAUUUCAGAUAAACAAAUUUUUCUUAGAAUAAGUAUGUCUCCAUGCAACAUU 
                   
               
               
                 UGGGACAUACUUAUGCUAAAAUAUUAUUUGUGUUUCAUCUGAAAUUCAAAUUCAACUGGACAUCCUGUAUUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-5 MI0006366 (SEQ ID NO: 1517) 
                   
               
               
                 UGCCCGGCCUCCCAUUAAAUUGGUUUUUCAGACAAAUCACAAAUUUGUUUAGGUAUAAGUAUAUCCCAUGUAAUCUU 
                   
               
               
                 UGGGACAUACUUAUGCUAAAAUAAUUGUUCCUUGUUGAUUGGAAAUUUUAAUUUUAAUUAGGUGUCCUGUAUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-6 MI0006367 (SEQ ID NO: 1518) 
                   
               
               
                 AACAAAUAAUUUGGUAAUAUAUGUAUGGCCCACACAAUAUUUAGGACAACAAUAUUUGGGACAUACUUAUGCUAAAA 
                   
               
               
                 AAGUAUUUGUUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-7 MI0006368 (SEQ ID NO: 1519) 
                   
               
               
                 ACAACAUGUUUUUAGGACAUGUAUGUCUGGUGCAAUAAUUGGGACAUACUUAUGCUAAAAAAAUUAGUGUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-8 MI0006369 (SEQ ID NO: 1520) 
                   
               
               
                 CCCAUUUAAACUUGAAUUUCAUAUAAACACCGUAAUUUUCAGCAUUAGUGUAUCACAUGCAGUAUUUGGGACAUACU 
                   
               
               
                 UAUGCUAAAAAAUUAGGUGGUGUUGAUCUGAAAUUCCAGUGUAGAUGGGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1302-9 MI0015978 (SEQ ID NO: 1521) 
                   
               
               
                 GGAUGCCCAGCUAGUUUGAAUUUUAGAUAAACAACGAAUAAUUUCGUAGCAUAAAUAUGUCCCAAGCUUAGUUUGGG 
                   
               
               
                 ACAUACUUAUGCUAAAAAACAUUAUUGGUUGUUUAUCUGAGAUUCAGAAUUAAGCAUUUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1303 MI0006370 (SEQ ID NO: 1522) 
                   
               
               
                 GGCUGGGCAACAUAGCGAGACCUCAACUCUACAAUUUUUUUUUUUUUAAAUUUUAGAGACGGGGUCUUGCUCUGUUG 
                   
               
               
                 CCAGGCUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1304 MI0006371 (SEQ ID NO: 1523) 
                   
               
               
                 AAACACUUGAGCCCAGCGGUUUGAGGCUACAGUGAGAUGUGAUCCUGCCACAUCUCACUGUAGCCUCGAACCCCUGG 
                   
               
               
                 GCUCAAGUGAUUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1305 MI0006372 (SEQ ID NO: 1524) 
                   
               
               
                 AAGAUCCUGCUGUUUCUACCAUUAGUUUUGAAUGUUUAUUGUAAAGAUACUUUUCAACUCUAAUGGGAGAGACAGCA 
                   
               
               
                 GGAUUCUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1306 MI0006443 (SEQ ID NO: 1525) 
                   
               
               
                 GUGAGCAGUCUCCACCACCUCCCCUGCAAACGUCCAGUGGUGCAGAGGUAAUGGACGUUGGCUCUGGUGGUGAUGGA 
                   
               
               
                 CAGUCCGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1307 MI0006444 (SEQ ID NO: 1526) 
                   
               
               
                 CAUCAAGACCCAGCUGAGUCACUGUCACUGCCUACCAAUCUCGACCGGACCUCGACCGGCUCGUCUGUGUUGCCAAU 
                   
               
               
                 CGACUCGGCGUGGCGUCGGUCGUGGUAGAUAGGCGGUCAUGCAUACGAAUUUUCAGCUCUUGUUCUGGUGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1321 MI0006652 (SEQ ID NO: 1527) 
                   
               
               
                 ACAUUAUGAAGCAAGUAUUAUUAUCCCUGUUUUACAAAUAAGGAAAUAAACUCAGGGAGGUGAAUGUGAUCAAAGAU 
                   
               
               
                 AG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1322 MI0006653 (SEQ ID NO: 1528) 
                   
               
               
                 AGUAUCAUGAAUUAGAAACCUACUUAUUACAUAGUUUACAUAAGAAGCGUGAUGAUGCUGCUGAUGCUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1323 MI0003786 (SEQ ID NO: 1529) 
                   
               
               
                 ACUGAGGUCCUCAAAACUGAGGGGCAUUUUCUGUGGUUUGAAAGGAAAGUGCACCCAGUUUUGGGGAUGUCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1324 MI0006657 (SEQ ID NO: 1530) 
                   
               
               
                 CCUGAAGAGGUGCAUGAAGCCUGGUCCUGCCCUCACUGGGAACCCCCUUCCCUCUGGGUACCAGACAGAAUUCUAUG 
                   
               
               
                 CACUUUCCUGGAGGCUCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1343 MI0017320 (SEQ ID NO: 1531) 
                   
               
               
                 GCUGGCGUCGGUGCUGGGGAGCGGCCCCCGGGUGGGCCUCUGCUCUGGCCCCUCCUGGGGCCCGCACUCUCGCUCUG 
                   
               
               
                 GGCCCGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1468 MI0003782 (SEQ ID NO: 1532) 
                   
               
               
                 GGUGGGUGGUUUCUCCGUUUGCCUGUUUCGCUGAUGUGCAUUCAACUCAUUCUCAGCAAAAUAAGCAAAUGGAAAAU 
                   
               
               
                 UCGUCCAUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1469 MI0007074 (SEQ ID NO: 1533) 
                   
               
               
                 CUCGGCGCGGGGCGCGGGCUCCGGGUUGGGGCGAGCCAACGCCGGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1470 MI0007075 (SEQ ID NO: 1534) 
                   
               
               
                 GCCCUCCGCCCGUGCACCCCGGGGCAGGAGACCCCGCGGGACGCGCCGAGGUAGGGGGGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1471 MI0007076 (SEQ ID NO: 1535) 
                   
               
               
                 GCCCGCGUGUGGAGCCAGGUGUAGAGGCGGAGCACAGCUGGCUCUAAUUUGAGGGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1537 MI0007258 (SEQ ID NO: 1536) 
                   
               
               
                 ACAGCUGUAAUUAGUCAGUUUUCUGUCCUGUCCACACAGAAAACCGUCUAGUUACAGUUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1538 MI0007259 (SEQ ID NO: 1537) 
                   
               
               
                 GGGAACAGCAGCAACAUGGGCCUCGCUUCCUGCCGGCGCGGCCCGGGCUGCUGCUGUUCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1539 MI0007260 (SEQ ID NO: 1538) 
                   
               
               
                 GGCUCUGCGGCCUGCAGGUAGCGCGAAAGUCCUGCGCGUCCCAGAUGCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1587 MI0016905 (SEQ ID NO: 1539) 
                   
               
               
                 UUUGGGCUGGGCUGGGUUGGGCAGUUCUUCUGCUGGACUCACCUGUGACCAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1825 MI0008193 (SEQ ID NO: 1540) 
                   
               
               
                 AGAGACUGGGGUGCUGGGCUCCCCUAGACUAGGACUCCAGUGCCCUCCUCUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1827 MI0008195 (SEQ ID NO: 1541) 
                   
               
               
                 UCAGCAGCACAGCCUUCAGCCUAAAGCAAUGAGAAGCCUCUGAAAGGCUGAGGCAGUAGAUUGAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1908 MI0008329 (SEQ ID NO: 1542) 
                   
               
               
                 CGGGAAUGCCGCGGCGGGGACGGCGAUUGGUCCGUAUGUGUGGUGCCACCGGCCGCCGGCUCCGCCCCGGCCCCCGC 
                   
               
               
                 CCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1909 MI0008330 (SEQ ID NO: 1543) 
                   
               
               
                 CAUCCAGGACAAUGGUGAGUGCCGGUGCCUGCCCUGGGGCCGUCCCUGCGCAGGGGCCGGGUGCUCACCGCAUCUGC 
                   
               
               
                 CCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1910 MI0008331 (SEQ ID NO: 1544) 
                   
               
               
                 UGUCCCUUCAGCCAGUCCUGUGCCUGCCGCCUUUGUGCUGUCCUUGGAGGGAGGCAGAAGCAGGAUGACAAUGAGGG 
                   
               
               
                 CAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1911 MI0008332 (SEQ ID NO: 1545) 
                   
               
               
                 UCGGCAUCUGCUGAGUACCGCCAUGUCUGUUGGGCAUCCACAGUCUCCCACCAGGCAUUGUGGUCUCCGCUGACGCU 
                   
               
               
                 UUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1912 MI0008333 (SEQ ID NO: 1546) 
                   
               
               
                 CUCUAGGAUGUGCUCAUUGCAUGGGCUGUGUAUAGUAUUAUUCAAUACCCAGAGCAUGCAGUGUGAACAUAAUAGAG 
                   
               
               
                 AUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1913 MI0008334 (SEQ ID NO: 1547) 
                   
               
               
                 ACCUCUACCUCCCGGCAGAGGAGGCUGCAGAGGCUGGCUUUCCAAAACUCUGCCCCCUCCGCUGCUGCCAAGUGGCU 
                   
               
               
                 GGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1914 MI0008335 (SEQ ID NO: 1548) 
                   
               
               
                 CGUGUGAGCCCGCCCUGUGCCCGGCCCACUUCUGCUUCCUCUUAGCGCAGGAGGGGUCCCGCACUGGGAGGGGCCCU 
                   
               
               
                 CAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1915 MI0008336 (SEQ ID NO: 1549) 
                   
               
               
                 UGAGAGGCCGCACCUUGCCUUGCUGCCCGGGCCGUGCACCCGUGGGCCCCAGGGCGACGCGGCGGGGGCGGCCCUAG 
                   
               
               
                 CGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1972-1 MI0009982 (SEQ ID NO: 1550) 
                   
               
               
                 UAUAGGCAUGUGCCACCACACCUGGCUUAAAUGUGUCAUUUAAAAAUUCAGGCCAGGCACAGUGGCUCAUGCCUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1972-2 MI0015977 (SEQ ID NO: 1551) 
                   
               
               
                 UAUAGGCAUGUGCCACCACACCUGGCUUAAAUGUGUCAUUUAAAAAUUCAGGCCAGGCACAGUGGCUCAUGCCUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1973 MI0009983 (SEQ ID NO: 1552) 
                   
               
               
                 UAUGUUCAACGGCCAUGGUAUCCUGACCGUGCAAAGGUAGCAUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-1976 MI0009986 (SEQ ID NO: 1553) 
                   
               
               
                 GCAGCAAGGAAGGCAGGGGUCCUAAGGUGUGUCCUCCUGCCCUCCUUGCUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2052 MI0010486 (SEQ ID NO: 1554) 
                   
               
               
                 CUGUUUUGAUAACAGUAAUGUCCCUUUAGUUCAAAGUUACCAGCUAUCAAAACAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2053 MI0010487 (SEQ ID NO: 1555) 
                   
               
               
                 CUUGCCAUGUAAAUACAGAUUUAAUUAACAUUUGCAACCUGUGAAGAUGCAAAACUUUAAGUGUUAAUUAAACCUCU 
                   
               
               
                 AUUUACAUAGCAAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2054 MI0010488 (SEQ ID NO: 1556) 
                   
               
               
                 CUGUAAUAUAAAUUUAAUUUAUUCUCUAUCAUUAAAAAAUGUAUUACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2110 MI0010629 (SEQ ID NO: 1557) 
                   
               
               
                 CAGGGGUUUGGGGAAACGGCCGCUGAGUGAGGCGUCGGCUGUGUUUCUCACCGCGGUCUUUUCCUCCCACUCUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2113 MI0003939 (SEQ ID NO: 1558) 
                   
               
               
                 UUUUCAAAGCAAUGUGUGACAGGUACAGGGACAAAUCCCGUUAAUAAGUAAGAGGAUUUGUGCUUGGCUCUGUCACA 
                   
               
               
                 UGCCACUUUGAAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2114 MI0010633 (SEQ ID NO: 1559) 
                   
               
               
                 CCUCCAUGCUCCUAGUCCCUUCCUUGAAGCGGUCGGAUAAUCACAUGACGAGCCUCAAGCAAGGGACUUCAAGCUGG 
                   
               
               
                 UGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2115 MI0010634 (SEQ ID NO: 1560) 
                   
               
               
                 ACUGUCAUCCCACUGCUUCCAGCUUCCAUGACUCCUGAUGGAGGAAUCACAUGAAUUCAUCAGAAUUCAUGGAGGCU 
                   
               
               
                 AGAAGCAGUAUGAGGAUCAUUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2116 MI0010635 (SEQ ID NO: 1561) 
                   
               
               
                 GACCUAGGCUAGGGGUUCUUAGCAUAGGAGGUCUUCCCAUGCUAAGAAGUCCUCCCAUGCCAAGAACUCCCAGACUA 
                   
               
               
                 GGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2117 MI0010636 (SEQ ID NO: 1562) 
                   
               
               
                 GCUCUGAUUUACUUCUGUCCGGCAUGGUGAACAGCAGGAUUGGCUGUAGCUGUUCUCUUUGCCAAGGACAGAUCUGA 
                   
               
               
                 UCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2276 MI0011282 (SEQ ID NO: 1563) 
                   
               
               
                 GUGUUCUUCCAGUCCGCCCUCUGUCACCUUGCAGACGGCUUUCUCUCCGAAUGUCUGCAAGUGUCAGAGGCGAGGAG 
                   
               
               
                 UGGCAGCUGCAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2277 MI0011284 (SEQ ID NO: 1564) 
                   
               
               
                 GUGCUUCCUGCGGGCUGAGCGCGGGCUGAGCGCUGCCAGUCAGCGCUCACAUUAAGGCUGACAGCGCCCUGCCUGGC 
                   
               
               
                 UCGGCCGGCGAAGCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2278 MI0011285 (SEQ ID NO: 1565) 
                   
               
               
                 GUGCUGCAGGUGUUGGAGAGCAGUGUGUGUUGCCUGGGGACUGUGUGGACUGGUAUCACCCAGACAGCUUGCACUGA 
                   
               
               
                 CUCCAGACCCUGCCGUCAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2355 MI0015873 (SEQ ID NO: 1566) 
                   
               
               
                 CAGACGUGUCAUCCCCAGAUACAAUGGACAAUAUGCUAUUAUAAUCGUAUGGCAUUGUCCUUGCUGUUUGGAGAUAA 
                   
               
               
                 UACUGCUGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2392 MI0016870 (SEQ ID NO: 1567) 
                   
               
               
                 AUGGUCCCUCCCAAUCCAGCCAUUCCUCAGACCAGGUGGCUCCCGAGCCACCCCAGGCUGUAGGAUGGGGGUGAGAG 
                   
               
               
                 GUGCUAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2467 MI0017432 (SEQ ID NO: 1568) 
                   
               
               
                 GGACAGGCACCUGAGGCUCUGUUAGCCUUGGCUCUGGGUCCUGCUCCUUAGAGCAGAGGCAGAGAGGCUCAGGGUCU 
                   
               
               
                 GUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2681 MI0012062 (SEQ ID NO: 1569) 
                   
               
               
                 GCCCCCUUUUCACGCAUUUGUGUUUUACCACCUCCAGGAGACUGCCCAAAGACUCUUCAGUAUCAUGGAGUUGGUAA 
                   
               
               
                 AGCACAGAUGCAUGAAUAAUUCAACGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2682 MI0012063 (SEQ ID NO: 1570) 
                   
               
               
                 ACCUUCCUGAAAGAGGUUGGGGCAGGCAGUGACUGUUCAGACGUCCAAUCUCUUUGGGACGCCUCUUCAGCGCUGUC 
                   
               
               
                 UUCCCUGCCUCUGCCUUUAGGACGAGUCUCAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2861 MI0013006 (SEQ ID NO: 1571) 
                   
               
               
                 GGCGCCUCUGCAGCUCCGGCUCCCCCUGGCCUCUCGGGAACUACAAGUCCCAGGGGGCCUGGCGGUGGGCGGCGGGC 
                   
               
               
                 GGAAGAGGCGGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2909 MI0013083 (SEQ ID NO: 1572) 
                   
               
               
                 GGUGUUAGGGCCAACAUCUCUUGGUCUUUCCCCUGUGGUCCCAAGAUGGCUGUUGCAACUUAACGCCAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-2964a MI0017299 (SEQ ID NO: 1573) 
                   
               
               
                 GGAGCUCAGCCACAGAUGUCCAGCCACAAUUCUCGGUUGGCCGCAGACUCGUACAAGAAUUGCGUUUGGACAAUCAG 
                   
               
               
                 UGGCGAAGCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3064 MI0017375 (SEQ ID NO: 1574) 
                   
               
               
                 GGUCUGGCUGUUGUGGUGUGCAAAACUCCGUACAUUGCUAUUUUGCCACACUGCAACACCUUACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3065 MI0014228 (SEQ ID NO: 1575) 
                   
               
               
                 CUGCCCUCUUCAACAAAAUCACUGAUGCUGGAGUCGCCUGAGUCAUCACUCAGCACCAGGAUAUUGUUGGAGAGGAC 
                   
               
               
                 AG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3074 MI0014181 (SEQ ID NO: 1576) 
                   
               
               
                 GCUCGACUCCUGUUCCUGCUGAACUGAGCCAGUGUGUAAAAUGAGAACUGAUAUCAGCUCAGUAGGCACCGGAGGGC 
                   
               
               
                 GGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3115 MI0014127 (SEQ ID NO: 1577) 
                   
               
               
                 UCUGAAUAUGGGUUUACUAGUUGGUGGUGAAUUCAUGAGUCGCCAACUAUUAGGCCUUUAUGUCCAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3116-1 MI0014128 (SEQ ID NO: 1578) 
                   
               
               
                 CUUUAUUGAGUCCCUACUAUGUUCCAGGCACUGGGUAUCGUAGGUGCCUGGAACAUAGUAGGGACUCAAUAAAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3116-2 MI0014129 (SEQ ID NO: 1579) 
                   
               
               
                 UAUUGAGUCCCUACUAUGUUCCAGGCACCUACGAUACCCAGUGCCUGGAACAUAGUAGGGACUCAAUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3117 MI0014130 (SEQ ID NO: 1580) 
                   
               
               
                 CCCUAAAGGGCCAGACACUAUACGAGUCAUAUAAGGGAAGGCAUUAUAGGACUCAUAUAGUGCCAGGUGUUUUGUGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3118-1 MI0014131 (SEQ ID NO: 1581) 
                   
               
               
                 CACACUACAAUAAUUUUCAUAAUGCAAUCACACAUAAUCACUAUGUGACUGCAUUAUGAAAAUUCUUGUAGUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3118-2 MI0014132 (SEQ ID NO: 1582) 
                   
               
               
                 ACACUACAAUAAUUUUCAUAAUGCAAUCACACAUAAUCACUAUGUGACUGCAUUAUGAAAAUUCUUGUAGUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3118-3 MI0014133 (SEQ ID NO: 1583) 
                   
               
               
                 CACACUACAAUAAUUUUCAUAAUGCAAUCACACAUAAUCACUAUGUGACUGCAUUAUGAAAAUUCUUGUAGUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3118-4 MI0014207 (SEQ ID NO: 1584) 
                   
               
               
                 CAUACUACAAUAAUUUUCAUAAUGCAAUCACACACAAUCACCGUGUGACUGCAUUAUGAAAAUUCUUCUAGUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3118-5 MI0014243 (SEQ ID NO: 1585) 
                   
               
               
                 CACACAUACAAUAAUAUUCAUAAUGCAAUCACACACAAUCACCAUGUGACUGCAUUAUGAAAAUUCUUCUAGUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3118-6 MI0015981 (SEQ ID NO: 1586) 
                   
               
               
                 CAUACUACAAUAAUUUUCAUAAUGCAAUCACACACAAUCACCGUGUGACUGCAUUAUGAAAAUUCUUCUAGUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3119-1 MI0014134 (SEQ ID NO: 1587) 
                   
               
               
                 AUUAACUCUGGCUUUUAACUUUGAUGGCAAAGGGGUAGCUAAACAAUCUAUGUCUUUGCCAUCAAAGUUAAAAGCCA 
                   
               
               
                 UAGUUAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3119-2 MI0014135 (SEQ ID NO: 1588) 
                   
               
               
                 AUUAACUAUGGCUUUUAACUUUGAUGGCAAAGACAUAGAUUGUUUAGCUACCCCUUUGCCAUCAAAGUUAAAAGCCA 
                   
               
               
                 GAGUUAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3120 MI0014136 (SEQ ID NO: 1589) 
                   
               
               
                 GUCAUGUGACUGCCUGUCUGUGCCUGCUGUACAGGUGAGCGGAUGUUCUGCACAGCAAGUGUAGACAGGCAGACACA 
                   
               
               
                 UGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3121 MI0014137 (SEQ ID NO: 1590) 
                   
               
               
                 AAAUGGUUAUGUCCUUUGCCUAUUCUAUUUAAGACACCCUGUACCUUAAAUAGAGUAGGCAAAGGACAGAAACAUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3122 MI0014138 (SEQ ID NO: 1591) 
                   
               
               
                 ACCAGCUCUGUUGGGACAAGAGGACGGUCUUCUUUUGGAAGGAAGACCAUCAUCUUGUCCGAAGAGAGCUGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3123 MI0014139 (SEQ ID NO: 1592) 
                   
               
               
                 AUGGAUUUGAUUGAAUGAUUCUCCCAUUUCCACAUGGAGAGUGGAGCCCAGAGAAUUGUUUAAUCAUGUAUCCAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3124 MI0014140 (SEQ ID NO: 1593) 
                   
               
               
                 GCGGGCUUCGCGGGCGAAGGCAAAGUCGAUUUCCAAAAGUGACUUUCCUCACUCCCGUGAAGUCGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3125 MI0014142 (SEQ ID NO: 1594) 
                   
               
               
                 GAGAAUGGGUAGAGGAAGCUGUGGAGAGAACUCACGGUGCCUGUGGUUCGAGAUCCCCGCCUUCCUCCUCCUUUCCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3126 MI0014143 (SEQ ID NO: 1595) 
                   
               
               
                 AUGAUUAUAUGAGGGACAGAUGCCAGAAGCACUGGUUAUGAUUUGCAUCUGGCAUCCGUCACACAGAUAAUUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3127 MI0014144 (SEQ ID NO: 1596) 
                   
               
               
                 GGCCAGGCCCAUCAGGGCUUGUGGAAUGGGAAGGAGAAGGGACGCUUCCCCUUCUGCAGGCCUGCUGGGUGUGGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3128 MI0014145 (SEQ ID NO: 1597) 
                   
               
               
                 UUCCUCUGGCAAGUAAAAAACUCUCAUUUUCCUUAAAAAAUGAGAGUUUUUUACUUGCAAUAGGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3129 MI0014146 (SEQ ID NO: 1598) 
                   
               
               
                 GUACUUGGGCAGUAGUGUAGAGAUUGGUUUGCCUGUUAAUGAAUUCAAACUAAUCUCUACACUGCUGCCCAAGAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3130-1 MI0014147 (SEQ ID NO: 1599) 
                   
               
               
                 CUUGUCAUGUCUUACCCAGUCUCCGGUGCAGCCUGUUGUCAAGGCUGCACCGGAGACUGGGUAAGACAUGACAAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3130-2 MI0014148 (SEQ ID NO: 1600) 
                   
               
               
                 CUUGUCAUGUCUUACCCAGUCUCCGGUGCAGCCUUGACAACAGGCUGCACCGGAGACUGGGUAAGACAUGACAAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3131 MI0014151 (SEQ ID NO: 1601) 
                   
               
               
                 GAGUCGAGGACUGGUGGAAGGGCCUUUCCCCUCAGACCAAGGCCCUGGCCCCAGCUUCUUCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3132 MI0014152 (SEQ ID NO: 1602) 
                   
               
               
                 GGUGGGAUGGGUAGAGAAGGAGCUCAGAGGACGGUGCGCCUUGUUUCCCUUGAGCCCUCCCUCUCUCAUCCCACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3133 MI0014153 (SEQ ID NO: 1603) 
                   
               
               
                 CAGAAAUUGUAAAGAACUCUUAAAACCCAAUAGUAAAAAGACAACCUGUUGAGUUUUAAGAGUUCUUUAUAUAUUCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3134 MI0014155 (SEQ ID NO: 1604) 
                   
               
               
                 UGUAUCCAAUGUGUAGUCUUUUAUCCCUCACAUGGAGUAAAAUAUGAUGGAUAAAAGACUACAUAUUGGGUACA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3135a MI0014156 (SEQ ID NO: 1605) 
                   
               
               
                 UCACUUUGGUGCCUAGGCUGAGACUGCAGUGGUGCAAUCUCAGUUCACUGCAGCCUUGACCUCCUGGGCUCAGGUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3135b MI0016809 (SEQ ID NO: 1606) 
                   
               
               
                 UGCCCAGGCUGGAGCGAGUGCAGUGGUGCAGUCAGUCCUAGCUCACUGCAGCCUCGAACUCCUGGGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3136 MI0014158 (SEQ ID NO: 1607) 
                   
               
               
                 AAUAUGAAACUGACUGAAUAGGUAGGGUCAUUUUUCUGUGACUGCACAUGGCCCAACCUAUUCAGUUAGUUCCAUAUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3137 MI0014160 (SEQ ID NO: 1608) 
                   
               
               
                 UACAGGUCUGUAGCCUGGGAGCAAUGGGGUGUAUGGUAUAGGGGUAGCCUCGUGCUCCUGGGCUACAAACCUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3138 MI0014161 (SEQ ID NO: 1609) 
                   
               
               
                 CCCUCCUCGGCACUUCCCCCACCUCACUGCCCGGGUGCCCACAAGACUGUGGACAGUGAGGUAGAGGGAGUGCCGAG 
                   
               
               
                 GAGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3139 MI0014162 (SEQ ID NO: 1610) 
                   
               
               
                 GGCUCAGAGUAGGAGCUCAACAGAUGCCUGUUGACUGAAUAAUAAACAGGUAUCGCAGGAGCUUUUGUUAUGUGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3140 MI0014163 (SEQ ID NO: 1611) 
                   
               
               
                 CCUCUUGAGGUACCUGAAUUACCAAAAGCUUUAUGUAUUCUGAAGUUAUUGAAAAUAAGAGCUUUUGGGAAUUCAGG 
                   
               
               
                 UAGUUCAGGAGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3141 MI0014165 (SEQ ID NO: 1612) 
                   
               
               
                 UCACCCGGUGAGGGCGGGUGGAGGAGGAGGGUCCCCACCAUCAGCCUUCACUGGGACGGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3142 MI0014166 (SEQ ID NO: 1613) 
                   
               
               
                 UUCAGAAAGGCCUUUCUGAACCUUCAGAAAGGCUGCUGAAUCUUCAGAAAGGCCUUUCUGAACCUUCAGAAAGGCUG 
                   
               
               
                 CUGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3143 MI0014167 (SEQ ID NO: 1614) 
                   
               
               
                 UAGAUAACAUUGUAAAGCGCUUCUUUCGCGGUUGGGCUGGAGCAACUCUUUACAAUGUUUCUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3144 MI0014169 (SEQ ID NO: 1615) 
                   
               
               
                 AACUACACUUUAAGGGGACCAAAGAGAUAUAUAGAUAUCAGCUACCUAUAUACCUGUUCGGUCUCUUUAAAGUGUAG 
                   
               
               
                 UU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3145 MI0014170 (SEQ ID NO: 1616) 
                   
               
               
                 UAUAUGAGUUCAACUCCAAACACUCAAAACUCAUUGUUGAAUGGAAUGAGAUAUUUUGAGUGUUUGGAAUUGAACUC 
                   
               
               
                 GUAUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3146 MI0014172 (SEQ ID NO: 1617) 
                   
               
               
                 GCUAAGUCCCUUCUUUCUAUCCUAGUAUAACUUGAAGAAUUCAAAUAGUCAUGCUAGGAUAGAAAGAAUGGGACUUG 
                   
               
               
                 GC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3147 MI0014173 (SEQ ID NO: 1618) 
                   
               
               
                 GUCCGGGUUGGGCAGUGAGGAGGGUGUGACGCCGCGAAGUGCACCUCGCCCUUGUCCAACUCGGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3148 MI0014175 (SEQ ID NO: 1619) 
                   
               
               
                 GAGUUAAGAUGGAAAAAACUGGUGUGUGCUUAUUGAUGUAGCCAACAAGCAUACAUCAGUUUUUUCCAACUUAACUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3149 MI0014176 (SEQ ID NO: 1620) 
                   
               
               
                 AUACAUACAUGUACACACACAUGUCAUCCACACACAUACAUAUAUAUAUGUUUGUAUGGAUAUGUGUGUGUAUGUGU 
                   
               
               
                 GUGUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3150a MI0014177 (SEQ ID NO: 1621) 
                   
               
               
                 GGGAAGCAGGCCAACCUCGACGAUCUCCUCAGCACCUGAACGCCAAGGCUGGGGAGAUCCUCGAGGUUGGCCUGCUU 
                   
               
               
                 UCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3150b MI0016426 (SEQ ID NO: 1622) 
                   
               
               
                 GAGGGAAAGCAGGCCAACCUCGAGGAUCUCCCCAGCCUUGGCGUUCAGGUGCUGAGGAGAUCGUCGAGGUUGGCCUG 
                   
               
               
                 CUUCCCCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3151 MI0014178 (SEQ ID NO: 1623) 
                   
               
               
                 GGGGUGAUGGGUGGGGCAAUGGGAUCAGGUGCCUCAAAGGGCAUCCCACCUGAUCCCACAGCCCACCUGUCACCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3152 MI0014179 (SEQ ID NO: 1624) 
                   
               
               
                 GUGCAGAGUUAUUGCCUCUGUUCUAACACAAGACUAGGCUUCCCUGUGUUAGAAUAGGGGCAAUAACUCUGCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3153 MI0014180 (SEQ ID NO: 1625) 
                   
               
               
                 GACAAAUUUUAAAUGUCCCUGUCCCCUUCCCCCCAAUUAAAGUAGAUUGGGGGAAAGCGAGUAGGGACAUUUAAAAU 
                   
               
               
                 UUGUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3154 MI0014182 (SEQ ID NO: 1626) 
                   
               
               
                 GGCCCCUCCUUCUCAGCCCCAGCUCCCGCUCACCCCUGCCACGUCAAAGGAGGCAGAAGGGGAGUUGGGAGCAGAGA 
                   
               
               
                 GGGGACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3155a MI0014183 (SEQ ID NO: 1627) 
                   
               
               
                 UCCGGGCAUCACCUCCCACUGCAGAGCCUGGGGAGCCGGACAGCUCCCUUCCCAGGCUCUGCAGUGGGAACUGAUGC 
                   
               
               
                 CUGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3155b MI0016839 (SEQ ID NO: 1628) 
                   
               
               
                 CCACUGCAGAGCCUGGGAAGGGAGCUGUCCGGCUCCCCAGGCUCUGCAGUGGGAGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3156-1 MI0014184 (SEQ ID NO: 1629) 
                   
               
               
                 GCAGAAGAAAGAUCUGGAAGUGGGAGACACUUUUACUAUAUAUAGUGGCUCCCACUUCCAGAUCUUUCUCUCUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3156-2 MI0014230 (SEQ ID NO: 1630) 
                   
               
               
                 UGCAGAAGAAAGAUCUGGAAGUGGGAGACACUUUCACUAUAUAUAGUGGCUCCCACUUCCAGAUCUUUCUCUCUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3156-3 MI0014242 (SEQ ID NO: 1631) 
                   
               
               
                 UGCAGAAGAAAGAUCUGGAAGUGGGAGACACUUUCACUAUAUAUAGUGGCUCCCACUUCCUGAUCUUUCUCUCUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3157 MI0014185 (SEQ ID NO: 16323) 
                   
               
               
                 GGGAAGGGCUUCAGCCAGGCUAGUGCAGUCUGCUUUGUGCCAACACUGGGGUGAUGACUGCCCUAGUCUAGCUGAAG 
                   
               
               
                 CUUUUCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3158-1 MI0014186 (SEQ ID NO: 1633) 
                   
               
               
                 AUUCAGGCCGGUCCUGCAGAGAGGAAGCCCUUCUGCUUACAGGUAUUGGAAGGGCUUCCUCUCUGCAGGACCGGCCU 
                   
               
               
                 GAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3158-2 MI0014187 (SEQ ID NO: 1634) 
                   
               
               
                 AUUCAGGCCGGUCCUGCAGAGAGGAAGCCCUUCCAAUACCUGUAAGCAGAAGGGCUUCCUCUCUGCAGGACCGGCCU 
                   
               
               
                 GAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3159 MI0014188 (SEQ ID NO: 1635) 
                   
               
               
                 CCAAAGUCCUAGGAUUACAAGUGUCGGCCACGGGCUGGGCACAGUGGCUCACGCCUGUAAUCCCAGCAUUUUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3160-1 MI0014189 (SEQ ID NO: 1636) 
                   
               
               
                 GGACCUGCCCUGGGCUUUCUAGUCUCAGCUCUCCUCCAGCUCAGCUGGUCAGGAGAGCUGAGACUAGAAAGCCCAGG 
                   
               
               
                 GCAGGUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3160-2 MI0014190 (SEQ ID NO: 1637) 
                   
               
               
                 ACCUGCCCUGGGCUUUCUAGUCUCAGCUCUCCUGACCAGCUGAGCUGGAGGAGAGCUGAGACUAGAAAGCCCAGGGC 
                   
               
               
                 AGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3161 MI0014191 (SEQ ID NO: 1638) 
                   
               
               
                 CCUCGAGAGCUGAUAAGAACAGAGGCCCAGAUUGAAGUUGAAUAGUGCUGGGCCUUUGUUUUUACCAAGUUCCCUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3162 MI0014192 (SEQ ID NO: 1639) 
                   
               
               
                 CUGACUUUUUUAGGGAGUAGAAGGGUGGGGAGCAUGAACAAUGUUUCUCACUCCCUACCCCUCCACUCCCCAAAAAA 
                   
               
               
                 GUCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3163 MI0014193 (SEQ ID NO: 1640) 
                   
               
               
                 UUCCUCAUCUAUAAAAUGAGGGCAGUAAGACCUUCCUUCCUUGUCUUACUACCCCCAUUUUAUAGAUGAGGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3164 MI0014194 (SEQ ID NO: 1641) 
                   
               
               
                 CUUGGAAACUGUGACUUUAAGGGAAAUGGCGCACAGCAGACCCUGCAAUCAUGCCGUUUUGCUUGAAGUCGCAGUUU 
                   
               
               
                 CCCAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3165 MI0014195 (SEQ ID NO: 1642) 
                   
               
               
                 CCGGUGGCAAGGUGGAUGCAAUGUGACCUCAACUCUUGGUCCUCUGAGGUCACAUUGUAUCCACCUUACCACUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3166 MI0014196 (SEQ ID NO: 1643) 
                   
               
               
                 AAAUUUUUUUGAGGCCAGUAGGCAUUGUCUGCGUUAGGAUUUCUGUAUCAUCCUCCUAACGCAGACAAUGCCUACUG 
                   
               
               
                 GCCUAAGAAAAAUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3167 MI0014198 (SEQ ID NO: 1644) 
                   
               
               
                 GGCUGUGGAGGCACCAGUAUUUCUGAAAUUCUUUUUUCUGAAAUUCUUCAGGAAGGAUUUCAGAAAUACUGGUGUCC 
                   
               
               
                 CGACAGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3168 MI0014199 (SEQ ID NO: 1645) 
                   
               
               
                 AAGAUCAUGAGUUCUACAGUCAGACAGCCUGAGUUGGAGGCUCAUCUUCACUUCUUGCUGUGUGACCCUGGGCCAGU 
                   
               
               
                 GACUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3169 MI0014200 (SEQ ID NO: 1646) 
                   
               
               
                 AUGUGAAAACAUAGGACUGUGCUUGGCACAUAGCACAAAGUCUUAUGGUACUGUGUGCCAAGCAUAGUCCUGUGUUU 
                   
               
               
                 UUACAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3170 MI0014201 (SEQ ID NO: 1647) 
                   
               
               
                 CUGGUAACACUGGGGUUCUGAGACAGACAGUGUUAGCUCCAGAAGCAUUGCCUGUCUUAGAACCCCUAUGUUACCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3171 MI0014202 (SEQ ID NO: 1648) 
                   
               
               
                 UAUAUAUAGAGAUGUAUGGAAUCUGUAUAUAUCUAUAUAUAUGUGUAUAUAUAGAUUCCAUAAAUCUAUAUAUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3173 MI0014204 (SEQ ID NO: 1649) 
                   
               
               
                 UCCCUGCCCUGCCUGUUUUCUCCUUUGUGAUUUUAUGAGAACAAAGGAGGAAAUAGGCAGGCCAGGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3174 MI0014208 (SEQ ID NO: 1650) 
                   
               
               
                 GUUACCUGGUAGUGAGUUAGAGAUGCAGAGCCCUGGGCUCCUCAGCAAACCUACUGGAUCUGCAUUUUAAUUCACAU 
                   
               
               
                 GCAUGGUAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3175 MI0014209 (SEQ ID NO: 1651) 
                   
               
               
                 CCUGGGGGGCGGGGAGAGAACGCAGUGACGUCUGGCCGCGUGCGCAUGUCGGGCGCUUUCUCCUCCCCCUACCCAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3176 MI0014210 (SEQ ID NO: 1652) 
                   
               
               
                 UGGCCUCUCCAGUCUGCAGCUCCCGGCAGCCUCGGGCCACACUCCCGGGAUCCCCAGGGACUGGCCUGGGACUACCG 
                   
               
               
                 GGGGUGGCGGCCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3177 MI0014211 (SEQ ID NO: 1653) 
                   
               
               
                 CCACGUGCCAUGUGUACACACGUGCCAGGCGCUGUCUUGAGACAUUCGCGCAGUGCACGGCACUGGGGACACGUGGC 
                   
               
               
                 ACUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3178 MI0014212 (SEQ ID NO: 1654) 
                   
               
               
                 GAGGCUGGGCGGGGCGCGGCCGGAUCGGUCGAGAGCGUCCUGGCUGAUGACGGUCUCCCGUGCCCACGCCCCAAACG 
                   
               
               
                 CAGUCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3179-1 MI0014213 (SEQ ID NO: 1655) 
                   
               
               
                 CAGGAUCACAGACGUUUAAAUUACACUCCUUCUGCUGUGCCUUACAGCAGUAGAAGGGGUGAAAUUUAAACGUCUGU 
                   
               
               
                 GAUCCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3179-2 MI0014216 (SEQ ID NO: 1656) 
                   
               
               
                 CAGGAUCACAGACGUUUAAAUUACACUCCUUCUGCUGUGCCUUACAGCAGUAGAAGGGGUGAAAUUUAAACGUCUGU 
                   
               
               
                 GAUCCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3179-3 MI0014221 (SEQ ID NO: 1657) 
                   
               
               
                 CAGGAUCACAGACGUUUAAAUUACACUCCUUCUGCUGUGCCUUACAGCAGUAGAAGGGGUGAAAUUUAAACGUCUGU 
                   
               
               
                 GAUCCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3180-1 MI0014214 (SEQ ID NO: 1658) 
                   
               
               
                 CAGUGCGACGGGCGGAGCUUCCAGACGCUCCGCCCCACGUCGCAUGCGCCCCGGGAAAGCGUGGGGCGGAGCUUCCG 
                   
               
               
                 GAGGCCCCGCCCUGCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3180-2 MI0014215 (SEQ ID NO: 1659) 
                   
               
               
                 GCGACGGGCGGAGCUUCCAGACGCUCCGCCCCACGUCGCAUGCGCCCCGGGAAAGCGUGGGGCGGAGCUUCCGGAGG 
                   
               
               
                 CCCCGCCCUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3180-3 MI0014217 (SEQ ID NO: 1660) 
                   
               
               
                 CAGUGCGACGGGCGGAGCUUCCAGACGCUCCGCCCCACGUCGCAUGCGCCCCGGGAAAGCGUGGGGCGGAGCUUCCG 
                   
               
               
                 GAGGCCCCGCCCUGCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3180-4 MI0016408 (SEQ ID NO: 1661) 
                   
               
               
                 GCUCCGCCCCACGUCGCAUGCGCCCCGGGAACGCGUGGGGCGGAGCUUCCGGAGGCCCCGCUCUGCUGCCGACCCUG 
                   
               
               
                 UGGAGCGGAGGGUGAAGCCUCCGGAUGCCAGUCCCUCAUCGCUGGCCUGGUCGCGCUGUGGCGAAGGGGGCGGAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3180-5 MI0016409 (SEQ ID NO: 1662) 
                   
               
               
                 GCUCCGCCCCACGUCGCAUGCGCCCCGGGAACGCGUGGGGCGGAGCUUCCGGAGGCCCCGCCCUGCUGCCGACCCUG 
                   
               
               
                 UGGAGCGGAGGGUGAAGCCUCCGGAUGCCAGUCCCUCAUCGCUGGCCCGGUCGCGCUGUGGCGAAGGGGGCGGAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3181 MI0014223 (SEQ ID NO: 1663) 
                   
               
               
                 CGGCGACCAUCGGGCCCUCGGCGCCGGCCCGUUAGUUGCCCGGGCCCGAGCCGGCCGGGCCCGCGGGUUGCCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3182 MI0014224 (SEQ ID NO: 1664) 
                   
               
               
                 GCUGCUUCUGUAGUGUAGUCCGUGCAUCCGCCCUUCGAUGCUUGGGUUGGAUCAUAGAGCAGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3183 MI0014225 (SEQ ID NO: 1665) 
                   
               
               
                 CUCUGCCCUGCCUCUCUCGGAGUCGCUCGGAGCAGUCACGUUGACGGAAUCCUCCGGCGCCUCCUCGAGGGAGGAGA 
                   
               
               
                 GGCAGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3184 MI0014226 (SEQ ID NO: 1666) 
                   
               
               
                 AAGCAAGACUGAGGGGCCUCAGACCGAGCUUUUGGAAAAUAGAAAAGUCUCGCUCUCUGCCCCUCAGCCUAACUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3185 MI0014227 (SEQ ID NO: 1667) 
                   
               
               
                 GAAUGGAAGAAGAAGGCGGUCGGUCUGCGGGAGCCAGGCCGCAGAGCCAUCCGCCUUCUGUCCAUGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3186 MI0014229 (SEQ ID NO: 1668) 
                   
               
               
                 AGCCUGCGGUUCCAACAGGCGUCUGUCUACGUGGCUUCAACCAAGUUCAAAGUCACGCGGAGAGAUGGCUUUGGAAC 
                   
               
               
                 CAGGGGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3187 MI0014231 (SEQ ID NO: 1669) 
                   
               
               
                 GCUGGCCCUGGGCAGCGUGUGGCUGAAGGUCACCAUGUUCUCCUUGGCCAUGGGGCUGCGCGGGGCCAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3188 MI0014232 (SEQ ID NO: 1670) 
                   
               
               
                 GGCGCCUCCUGCUCUGCUGUGCCGCCAGGGCCUCCCCUAGCGCGCCUUCUGGAGAGGCUUUGUGCGGAUACGGGGCU 
                   
               
               
                 GGAGGCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3189 MI0014233 (SEQ ID NO: 1671) 
                   
               
               
                 GCCUCAGUUGCCCCAUCUGUGCCCUGGGUAGGAAUAUCCUGGAUCCCCUUGGGUCUGAUGGGGUAGCCGAUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3190 MI0014235 (SEQ ID NO: 1672) 
                   
               
               
                 CUGGGGUCACCUGUCUGGCCAGCUACGUCCCCACGGCCCUUGUCAGUGUGGAAGGUAGACGGCCAGAGAGGUGACCC 
                   
               
               
                 CGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3191 MI0014236 (SEQ ID NO: 1673) 
                   
               
               
                 GGGGUCACCUCUCUGGCCGUCUACCUUCCACACUGACAAGGGCCGUGGGGACGUAGCUGGCCAGACAGGUGACCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3192 MI0014237 (SEQ ID NO: 1674) 
                   
               
               
                 GGAAGGGAUUCUGGGAGGUUGUAGCAGUGGAAAAAGUUCUUUUCUUCCUCUGAUCGCCCUCUCAGCUCUUUCCUUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3193 MI0014238 (SEQ ID NO: 1675) 
                   
               
               
                 UCCUGCGUAGGAUCUGAGGAGUGGACGAGUCUCAUUACCCAGCUCCUGAGCAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3194 MI0014239 (SEQ ID NO: 1676) 
                   
               
               
                 AGGUGGCAGGGCCAGCCACCAGGAGGGCUGCGUGCCACCCGGGCAGCUCUGCUGCUCACUGGCAGUGUCACCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3195 MI0014240 (SEQ ID NO: 1677) 
                   
               
               
                 CCGCAGCCGCCGCGCCGGGCCCGGGUUGGCCGCUGACCCCCGCGGGGCCCCCGGCGGCCGGGGCGGGGGCGGGGGCU 
                   
               
               
                 GCCCCGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3196 MI0014241 (SEQ ID NO: 1678) 
                   
               
               
                 GGGUGGGGGCGGGGCGGCAGGGGCCUCCCCCAGUGCCAGGCCCCAUUCUGCUUCUCUCCCAGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3197 MI0014245 (SEQ ID NO: 1679) 
                   
               
               
                 GGCGAGGGGAGGCGCAGGCUCGGAAAGGCGCGCGAGGCUCCAGGCUCCUUCCCGAUCCACCGCUCUCCUCGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3198-1 MI0014246 (SEQ ID NO: 1680) 
                   
               
               
                 GACUGUGCUCUCACUGUUCACCCAGCACUAGCAGUACCAGACGGUUCUGUGGAGUCCUGGGGAAUGGAGAGAGCACA 
                   
               
               
                 GUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3198-2 MI0017335 (SEQ ID NO: 1681) 
                   
               
               
                 GACUCUGCUCUCACUGUUCACCCAGCACUAGCAGUACCAGAUGGUUCUGUGGAGUCCUGGGGAAUGGAGAGAGCACA 
                   
               
               
                 GUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3199-1 MI0014247 (SEQ ID NO: 1682) 
                   
               
               
                 GGUGACUCCAGGGACUGCCUUAGGAGAAAGUUUCUGGAAGUUCUGACAUUCCAGAAACUUUCUCCUAAGGCAGUCCC 
                   
               
               
                 UGGGAGUCACU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3199-2 MI0014248 (SEQ ID NO: 1683) 
                   
               
               
                 GUGACUCCCAGGGACUGCCUUAGGAGAAAGUUUCUGGAAUGUCAGAACUUCCAGAAACUUUCUCCUAAGGCAGUCCC 
                   
               
               
                 UGGAGUCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3200 MI0014249 (SEQ ID NO: 1684) 
                   
               
               
                 GGUGGUCGAGGGAAUCUGAGAAGGCGCACAAGGUUUGUGUCCAAUACAGUCCACACCUUGCGCUACUCAGGUCUGCU 
                   
               
               
                 CGUGCCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3201 MI0014250 (SEQ ID NO: 1685) 
                   
               
               
                 GGGAUAUGAAGAAAAAUAAGAGGCUAGGAUUGCCUCUUAUUUUUACAUGCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3202-1 MI0014252 (SEQ ID NO: 1686) 
                   
               
               
                 UAUUAAUAUGGAAGGGAGAAGAGCUUUAAUGAUUGGAGUCAUUUUCAGAGCAUUAAAGCUCUUCUCCCUUCCAUAUU 
                   
               
               
                 AAUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3202-2 MI0014253 (SEQ ID NO: 1687) 
                   
               
               
                 AUUAAUAUGGAAGGGAGAAGAGCUUUAAUGCUCUGAAAAUGACUCCAAUCAUUAAAGCUCUUCUCCCUUCCAUAUUA 
                   
               
               
                 AU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3529 MI0017351 (SEQ ID NO: 1688) 
                   
               
               
                 GGCACCAUUAGGUAGACUGGGAUUUGUUGUUGAGCGCAGUAAGACAACAACAAAAUCACUAGUCUUCCAGAUGGGGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3545 MI0017343 (SEQ ID NO: 1689) 
                   
               
               
                 GCGCCCGCCGGGUCUAGUGGUCCUAAACAUUUCACAAUUGCGCUACAGAACUGUUGAACUGUUAAGAACCACUGGAC 
                   
               
               
                 CCAGCGCGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3591 MI0017383 (SEQ ID NO: 1690) 
                   
               
               
                 CAGUAGCUAUUUAGUGUGAUAAUGGCGUUUGAUAGUUUAGACACAAACACCAUUGUCACACUCCACAGCUCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3605 MI0015995 (SEQ ID NO: 1691) 
                   
               
               
                 ACUUUAUACGUGUAAUUGUGAUGAGGAUGGAUAGCAAGGAAGCCGCUCCCACCUGACCCUCACGGCCUCCGUGUUAC 
                   
               
               
                 CUGUCCUCUAGGUGGGACGCUCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3606 MI0015996 (SEQ ID NO: 1692) 
                   
               
               
                 UUGUUGCUAUCUAGGUUAGUGAAGGCUAUUUUAAUUUUUUUAAAAUUUCUUUCACUACUUAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3607 MI0015997 (SEQ ID NO: 1693) 
                   
               
               
                 AAGGUUGCGGUGCAUGUGAUGAAGCAAAUCAGUAUGAAUGAAUUCAUGAUACUGUAAACGCUUUCUGAUGUACUACU 
                   
               
               
                 CA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3609 MI0015999 (SEQ ID NO: 1694) 
                   
               
               
                 GUAACAGUAACUUUUAUUCUCAUUUUCCUUUUCUCUACCUUGUAGAGAAGCAAAGUGAUGAGUAAUACUGGCUGGAG 
                   
               
               
                 CCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3610 MI0016000 (SEQ ID NO: 1695) 
                   
               
               
                 AAGAGCCGCGGCGUAACGGCAGCCAUCUUGUUUGUUUGAGUGAAUCGGAAAGGAGGCGCCGGCUGUGGCGGCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3611 MI0016001 (SEQ ID NO: 1696) 
                   
               
               
                 AGCAGGUCUAAUAAGAAUUUCUUUUUCUUCACAAUUAUGAAAGAAAAGAAAUUGUGAAGAAAGAAAUUCUUACUAGU 
                   
               
               
                 UUUGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3612 MI0016002 (SEQ ID NO: 1697) 
                   
               
               
                 GGGACUGGGGAUGAGGAGGCAUCUUGAGAAAUGGAAGGAAUGGGAUCUACUUCCAGUUCACUAGAGGCGUCCUGACA 
                   
               
               
                 CCCCUAGCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3613 MI0016003 (SEQ ID NO: 1698) 
                   
               
               
                 UGGUUGGGUUUGGAUUGUUGUACUUUUUUUUUUGUUCGUUGCAUUUUUAGGAACAAAAAAAAAAGCCCAACCCUUCA 
                   
               
               
                 CACCACUUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3614 MI0016004 (SEQ ID NO: 1699) 
                   
               
               
                 GGUUCUGUCUUGGGCCACUUGGAUCUGAAGGCUGCCCCUUUGCUCUCUGGGGUAGCCUUCAGAUCUUGGUGUUUUGA 
                   
               
               
                 AUUCUUACU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3615 MI0016005 (SEQ ID NO: 1700) 
                   
               
               
                 GACUCUGGGACGCUCAGACGCCGCGCGGGGCGGGGAUUGGUCUGUGGUCCUCUCUCGGCUCCUCGCGGCUCGCGGCG 
                   
               
               
                 GCCGACGGUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3616 MI0016006 (SEQ ID NO: 1701) 
                   
               
               
                 UGUCACUCCGCCAGCAUCAUGAAGUGCACUCAUGAUAUGUUUGCCCCAUCAGCGUGUCACGAGGGCAUUUCAUGAUG 
                   
               
               
                 CAGGCGGGGUUGGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3617 MI0016007 (SEQ ID NO: 1702) 
                   
               
               
                 AGGUCAUAGAAAGACAUAGUUGCAAGAUGGGAUUAGAAACCAUAUGUCUCAUCAGCACCCUAUGUCCUUUCUCUGCC 
                   
               
               
                 CU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3618 MI0016008 (SEQ ID NO: 1703) 
                   
               
               
                 UAAGCUGAGUGCAUUGUGAUUUCCAAUAAUUGAGGCAGUGGUUCUAAAAGCUGUCUACAUUAAUGAAAAGAGCAAUG 
                   
               
               
                 UGGCCAGCUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3619 MI0016009 (SEQ ID NO: 1704) 
                   
               
               
                 ACGGCAUCUUUGCACUCAGCAGGCAGGCUGGUGCAGCCCGUGGUGGGGGACCAUCCUGCCUGCUGUGGGGUAAGGAC 
                   
               
               
                 GGCUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3620 MI0016011 (SEQ ID NO: 1705) 
                   
               
               
                 GUGAGGUGGGGGCCAGCAGGGAGUGGGCUGGGCUGGGCUGGGCCAAGGUACAAGGCCUCACCCUGCAUCCCGCACCC 
                   
               
               
                 AG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3621 MI0016012 (SEQ ID NO: 1706) 
                   
               
               
                 GUGAGCUGCUGGGGACGCGGGUCGGGGUCUGCAGGGCGGUGCGGCAGCCGCCACCUGACGCCGCGCCUUUGUCUGUG 
                   
               
               
                 UCCCACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3622a MI0016013 (SEQ ID NO: 1707) 
                   
               
               
                 AAUAGAGGGUGCACAGGCACGGGAGCUCAGGUGAGGCAGGGAGCUGAGCUCACCUGACCUCCCAUGCCUGUGCACCC 
                   
               
               
                 UCUAUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3622b MI0016014 (SEQ ID NO: 1708) 
                   
               
               
                 AGUGAUAUAAUAGAGGGUGCACAGGCAUGGGAGGUCAGGUGAGCUCAGCUCCCUGCCUCACCUGAGCUCCCGUGCCU 
                   
               
               
                 GUGCACCCUCUAUUGGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3646 MI0016046 (SEQ ID NO: 1709) 
                   
               
               
                 UUCAGUAGGUUGGGUUCAUUUCAUUUUCAUGACAACCCUAUAUGGGAAAAUGUUGUGAAAAUGAAAUGAGCCCAGCC 
                   
               
               
                 CAUUGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3648 MI0016048 (SEQ ID NO: 1710) 
                   
               
               
                 CGCGACUGCGGCGGCGGUGGUGGGGGGAGCCGCGGGGAUCGCCGAGGGCCGGUCGGCCGCCCCGGGUGCCGCGCGGU 
                   
               
               
                 GCCGCCGGCGGCGGUGAGGCCCCGCGCGUGUGUCCCGGCUGCGGUCGGCCGCGCUCGAGGGGUCCCCGUGGCGUCCC 
                   
               
               
                 CUUCCCCGCCGGCCGCCUUUCUCGCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3649 MI0016049 (SEQ ID NO: 1711) 
                   
               
               
                 GCUUGGAACAGGCACCUGUGUGUGCCCAAGUGUUUCUAGCAAACACAGGGACCUGAGUGUCUAAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3650 MI0016050 (SEQ ID NO: 1712) 
                   
               
               
                 UCAAGGUGUGUCUGUAGAGUCCUGACUGCGUGCCAGGGGCUCUGUCUGGCACAUUUCUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3651 MI0016051 (SEQ ID NO: 1713) 
                   
               
               
                 GAUUCGAUGGGCCAUAGCAAUCCUGUGAUUUAUGCAUGGAGGCUGCUUCUCCUCAGCAGCUGCCAUAGCCCGGUCGC 
                   
               
               
                 UGGUACAUGAUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3652 MI0016052 (SEQ ID NO: 1714) 
                   
               
               
                 CGGCUGGAGGUGUGAGGAUCCGAACCCAGGGGUGGGGGGUGGAGGCGGCUCCUGCGAUCGAAGGGGACUUGAGACUC 
                   
               
               
                 ACCGGCCGCACGCCAUGAGGGCCCUGUGGGUGCUGGGCCUCUGCUGCGUCCUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3653 MI0016053 (SEQ ID NO: 1715) 
                   
               
               
                 UCCCUGGGGACCCCUGGCAGCCCCUCCUGAUGAUUCUUCUUCCUGAGCACGCUCAUGAUGAGCAAACUGAGCCUCUA 
                   
               
               
                 AGAAGUUGACUGAAGGGGCUGCUUCCCCAAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3654 MI0016054 (SEQ ID NO: 1716) 
                   
               
               
                 UUCAUGAGCUGCAAUCUCAUCACUGGAAUGUUCCAGCGACUGGACAAGCUGAGGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3655 MI0016055 (SEQ ID NO: 1717) 
                   
               
               
                 GCUUGUCGCUGCGGUGUUGCUGUUGGAGACUCGAUUGUUGGUGACAGCGAAAGAACGAUAACAAAAUGCCGGAGCGA 
                   
               
               
                 GAUAGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3656 MI0016056 (SEQ ID NO: 1718) 
                   
               
               
                 CUUUCGGCCAGCGGGACGGCAUCCGAGGUGGGCUAGGCUCGGGCCCGUGGCGGGUGCGGGGGUGGGAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3657 MI0016057 (SEQ ID NO: 1719) 
                   
               
               
                 UGUGUCCCAUAAUUAAAUAAUGAAAUCUGAAAUCACCAAUAAUGGGACACUAAUGUGAUUAAUGUUGUUGUGUCCCA 
                   
               
               
                 UUAUUGGUGAUUUCAGAUUUCAUAUAUGAUUAAGGACAUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3658 MI0016058 (SEQ ID NO: 1720) 
                   
               
               
                 UAUUUAAGAAAACACCAUGGAGAUGAAAUGCCUUUGAUUUUUUUUUUCUUUUUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3659 MI0016060 (SEQ ID NO: 1721) 
                   
               
               
                 UCUACAAGCAGAUACAAGGAUGCCCUUGUACACAACACACGUGCUGCUUGUAUAGACAUGAGUGUUGUCUACGAGGG 
                   
               
               
                 CAUCCUUGUGUCUGUGUGUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3660 MI0016061 (SEQ ID NO: 1722) 
                   
               
               
                 GAAAGAAGAACUGGACAAAAUUAAAAUGCUCUUCUGUCAUUGUAAUAGUUCAUAUGGGCACUGACAGGAGAGCAUUU 
                   
               
               
                 UGACUUUGUCAAGUGUGUCUGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3661 MI0016062 (SEQ ID NO: 1723) 
                   
               
               
                 CACCUUCUCGCAGAGGCUCUUGACCUGGGACUCGGACAGCUGCUUGCACUCGUUCAGCUGCUCGAUCCACUGGUCCA 
                   
               
               
                 GCUCCUUGGUGAACACCUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3662 MI0016063 (SEQ ID NO: 1724) 
                   
               
               
                 UGUGUUUUCCUCAACGCUCACAGUUACACUUCUUACUCUCAAUCCAUUCAUAUUGAAAAUGAUGAGUAGUGACUGAU 
                   
               
               
                 GAAGCACAAAUCAGCCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3663 MI0016064 (SEQ ID NO: 1725) 
                   
               
               
                 CCCGGGACCUUGGUCCAGGCGCUGGUCUGCGUGGUGCUCGGGUGGAUAAGUCUGAUCUGAGCACCACACAGGCCGGG 
                   
               
               
                 CGCCGGGACCAAGGGGGCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3664 MI0016065 (SEQ ID NO: 1726) 
                   
               
               
                 CUGUAAACUUGAAGGUAGGGAACUCUGUCUUCACUCAUGAGUACCUUCCAACACGAGCUCUCAGGAGUAAAGACAGA 
                   
               
               
                 GUUCCCUACCUUCAAUGUGGAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3665 MI0016066 (SEQ ID NO: 1727) 
                   
               
               
                 GCGGGCGGCGGCGGCGGCAGCAGCAGCAGGUGCGGGGCGGCGGCCGCGCUGGCCGCUCGACUCCGCAGCUGCUCGUU 
                   
               
               
                 CUGCUUCUCCAGCUUGCGCACCAGCUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3666 MI0016067 (SEQ ID NO: 1728) 
                   
               
               
                 AGUAAGGUCCGUCAGUUGUAAUGAGACCCAGUGCAAGUGUAGAUGCCGACUCCGUGGCAGAGUUCAGCGUUUCACAC 
                   
               
               
                 UGCCUGGUCUCUGUCACUCUAUUGAAUUAGAUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3667 MI0016068 (SEQ ID NO: 1729) 
                   
               
               
                 UGAGGAUGAAAGACCCAUUGAGGAGAAGGUUCUGCUGGCUGAGAACCUUCCUCUCCAUGGGUCUUUCAUCCUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3668 MI0016069 (SEQ ID NO: 1730) 
                   
               
               
                 AUAUAUGAAAUGUAGAGAUUGAUCAAAAUAGUUUCUAUCAAAAUAGUUUUGAUCAAUCUCUGCAAUUUUAUAUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3669 MI0016070 (SEQ ID NO: 1731) 
                   
               
               
                 AUAUAUAUAUACGGAAUAUAUAUACGGAAUAUAUAUAUACGGAAUAUAUAUAUACGGAAUAUGUAUACGGAAUAUAU 
                   
               
               
                 AUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3670-1 MI0016071 (SEQ ID NO: 1732) 
                   
               
               
                 UCUAGACUGGUAUAGCUGCUUUUGGAGCCUCACCUGCUGAGAGCUCACAGCUGUCCUUCUCUAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3670-2 MI0019112 (SEQ ID NO: 1733) 
                   
               
               
                 UCUAGACUGGUAUAGCUGCUUUUGGAGCCUCACCUGCUGAGAGCUCACAGCUGUCCUUCUCUAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3671 MI0016072 (SEQ ID NO: 1734) 
                   
               
               
                 AUGUUAUUGCUGCUGCUGUCACAUUUACAUGAAAAUAAAAUGUAAAUUAUUUUAUUUCUAUCAAAUAAGGACUAGUC 
                   
               
               
                 UGCAGUGAUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3672 MI0016073 (SEQ ID NO: 1735) 
                   
               
               
                 UCUUUGUGAUUACCAUGAGACUCAUGUAAAACAUCUUAGACUAUUACAAGAUGUUUUAUGAGUCUCAUGAUAAUCAC 
                   
               
               
                 AAAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3673 MI0016074 (SEQ ID NO: 1736) 
                   
               
               
                 AUAUAUAUAUAUGGAAUGUAUAUACGGAAUAUAUAUAUAUAUGGAAUGUAUAUACGGAAUAUAUAUAUAUAUGGAAU 
                   
               
               
                 GUAUAUACGGAAUAUAUAUAUAUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3674 MI0016075 (SEQ ID NO: 1737) 
                   
               
               
                 ACAUCACUAUUGUAGAACCUAAGAUUGGCCGUUUGAGAUGUCCUUUCAAGUUUUUGCAUUUCUGAUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3675 MI0016076 (SEQ ID NO: 1738) 
                   
               
               
                 GGAUGAUAAGUUAUGGGGCUUCUGUAGAGAUUUCUAUGAGAACAUCUCUAAGGAACUCCCCCAAACUGAAUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3676 MI0016077 (SEQ ID NO: 1739) 
                   
               
               
                 UUGGUUAAAGCGCCUGUCUAGUAAACAGGAGAUCCUGGGUUCGAAUCCCAGCGGUGCCUCCGUGUUUCCCCCACGCU 
                   
               
               
                 UUUGCCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3677 MI0016078 (SEQ ID NO: 1740) 
                   
               
               
                 GGCAGUGGCCAGAGCCCUGCAGUGCUGGGCAUGGGCUUCUCGUGGGCUCUGGCCACGGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3678 MI0016079 (SEQ ID NO: 1741) 
                   
               
               
                 GAAUCCGGUCCGUACAAACUCUGCUGUGUUGAAUGAUUGGUGAGUUUGUUUGCUCAUUGAUUGAAUCACUGCAGAGU 
                   
               
               
                 UUGUACGGACCGGAUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3679 MI0016080 (SEQ ID NO: 1742) 
                   
               
               
                 CGUGGUGAGGAUAUGGCAGGGAAGGGGAGUUUCCCUCUAUUCCCUUCCCCCCAGUAAUCUUCAUCAUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3680-1 MI0016081 (SEQ ID NO: 1743) 
                   
               
               
                 AAAUUUAAGGAGGGACUCACUCACAGGAUUGUGCAAAUGCAAAGUUGGCUUUUGCAUGACCCUGGGAGUAGGUGCCU 
                   
               
               
                 CCUUAAAUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3680-2 MI0019113 (SEQ ID NO: 1744) 
                   
               
               
                 AAAUUUAAGGAGGGACUCACUCACAGGAUUGUGCAAAUGCAAAGUUGGCUUUUGCAUGACCCUGGGAGUAGGUGCCU 
                   
               
               
                 CCUUAAAUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3681 MI0016082 (SEQ ID NO: 1745) 
                   
               
               
                 ACUUCCAGUAGUGGAUGAUGCACUCUGUGCAGGGCCAACUGUGCACACAGUGCUUCAUCCACUACUGGAAGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3682 MI0016083 (SEQ ID NO: 1746) 
                   
               
               
                 UAAGUUAUAUAUGUCUACUUCUACCUGUGUUAUCAUAAUAAAGGUGUCAUGAUGAUACAGGUGGAGGUAGAAAUAUA 
                   
               
               
                 UAACUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3683 MI0016084 (SEQ ID NO: 1747) 
                   
               
               
                 GGGUGUACACCCCCUGCGACAUUGGAAGUAGUAUCAUCUCUCCCUUGGAUGCUACGAACAAUAUCACAGAAGGUGUA 
                   
               
               
                 CACCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3684 MI0016085 (SEQ ID NO: 1748) 
                   
               
               
                 AAUCUAAAGGACCUGUACUAGGUUUAACAUGUUGAGCAUUACUCAUGUUAGACCUAGUACACGUCCUUUAGAUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3685 MI0016086 (SEQ ID NO: 1749) 
                   
               
               
                 GUACAUUUCCUACCCUACCUGAAGACUUGAGAUUAUAGUCUUUGGGGGGAUGGGCAAAGUAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3686 MI0016087 (SEQ ID NO: 1750) 
                   
               
               
                 CUCACCUCAUUCAUUUACCUUCUCUUACAGAUCACUUUUCUGCACUGGACAGUGAUCUGUAAGAGAAAGUAAAUGAA 
                   
               
               
                 AGAGGUGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3687 MI0016088 (SEQ ID NO: 1751) 
                   
               
               
                 CGCGCGUGCGCCCGAGCGCGGCCCGGUGGUCCCUCCCGGACAGGCGUUCGUGCGACGUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3688-1 MI0016089 (SEQ ID NO: 1752) 
                   
               
               
                 UCUUCACUUUCAAGAGUGGCAAAGUCUUUCCAUAUGUAUGUAUGUAUGUCUGUUACACAUAUGGAAAGACUUUGCCA 
                   
               
               
                 CUCUUUAAAGUGAAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3688-2 MI0017447 (SEQ ID NO: 1753) 
                   
               
               
                 UCACUUUAAAGAGUGGCAAAGUCUUUCCAUAUGUGUAACAGACAUACAUACAUACAUAUGGAAAGACUUUGCCACUC 
                   
               
               
                 UUGAAAGUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3689a MI0016090 (SEQ ID NO: 1754) 
                   
               
               
                 CCUGGGAGGUGUGAUAUCAUGGUUCCUGGGAGGUGUGAUCCUGUGCUUCCUGGGAGGUGUGAUAUCGUGGUUCCUGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3689b MI0016411 (SEQ ID NO: 1755) 
                   
               
               
                 GAUCCUGUGCUCCCUGGGGGGUCUGAUCCUGUGCUUCCUGGGAGGUGUGAUAUCAUGGUUCCUGGGAGGUGUGAUCC 
                   
               
               
                 CGUGCUUCCUGGGAGGUGUGAUAUUGUGGUUCCUGGGAGGUGUGAUCCCGUGCUCCCUGGGAGGUGUGAUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3689c MI0016832 (SEQ ID NO: 1756) 
                   
               
               
                 GGGAGGUGUGAUAUCGUGGUUCCUGGGAGGUGUGAUAUCGUGGUUCCUGGGAGGUGUGAUAUUGUGGUUCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3689d-1 MI0016834 (SEQ ID NO: 1757) 
                   
               
               
                 UGGGAGGUGUGAUCUCACACUCGCUGGGAGGUGUGCUAUCGUCUUCCCCGGGAGGUGUGAUCCUGUUCUUCCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3689d-2 MI0016835 (SEQ ID NO: 1758) 
                   
               
               
                 ACUGGGAGGUGUGAUCUCACACUCGCUGGGAGGUGUGCUAUCGUCUUCCCUGGGAGGUGUGAUCCUGUUCUUCCUGA 
                   
               
               
                 GCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3689e MI0016836 (SEQ ID NO: 1759) 
                   
               
               
                 GGGAGGUGUGAUAUCAUGGUUCCUGGGAGGUAUGAUAUCGUGGUUCCUGGGAGGUGUGAUCCCGUGCUCCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3689f MI0016837 (SEQ ID NO: 1760) 
                   
               
               
                 AGGUGUGAUAUCGUGCUUCCUGGGACGUGUGAUGCUGUGCUUCCUGGGAGGUGUGAUCCCACACUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3690 MI0016091 (SEQ ID NO: 1761) 
                   
               
               
                 CCCAUCUCCACCUGGACCCAGCGUAGACAAAGAGGUGUUUCUACUCCAUAUCUACCUGGACCCAGUGUAGAUGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3691 MI0016092 (SEQ ID NO: 1762) 
                   
               
               
                 UUGAGGCACUGGGUAGUGGAUGAUGGAGACUCGGUACCCACUGCUGAGGGUGGGGACCAAGUCUGCGUCAUCCUCUC 
                   
               
               
                 CUCAGUGCCUCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3692 MI0016093 (SEQ ID NO: 1763) 
                   
               
               
                 CCAUUCCUGCUGGUCAGGAGUGGAUACUGGAGCAAUAGAUACAGUUCCACACUGACACUGCAGAAGUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3713 MI0016134 (SEQ ID NO: 1764) 
                   
               
               
                 GGUAUCCGUUUGGGGAUGGUUUCACUAUCCCCAGAUGGAUACCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3714 MI0016135 (SEQ ID NO: 1765) 
                   
               
               
                 GAAGGCAGCAGUGCUCCCCUGUGACGUGCUCCAUCACCGGGCAGGGAAGACACCGCUGCCACCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3907 MI0016410 (SEQ ID NO: 1766) 
                   
               
               
                 GGGUUGGAAAGCUGUAGGUGUGGAGGGGCAUGGAUACGGGGGCCAUGAGGGUGGGGUCCAGGCUGGACCAGGCCUGC 
                   
               
               
                 CCUGAGUCCCCCAGCAGGUGCUCCAGGCUGGCUCACACCCUCUGCCUCUCUCUCUUCCUUCCUGGCCCCAACCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3908 MI0016412 (SEQ ID NO: 1767) 
                   
               
               
                 GCCUGAGCAAUGUAGGUAGACUGUUUCUAAAAAAAUAAAAAGUUAAAAAAAUUUAUGUUAACGUGUAAUGUGUUUAC 
                   
               
               
                 UAAUUUUUUUUUUUUUUUUUGGAGACAGAGUCUCCCUCUGUCGCCAGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3909 MI0016413 (SEQ ID NO: 1768) 
                   
               
               
                 GGUAUGCUGUUGCGCUGUCCUUCCUCUGGGGAGCAGGCUCCGGGGGACAGGGAAAAGCACACAAGGAACUUGUCCUC 
                   
               
               
                 UAGGGCCUGCAGUCUCAUGGGAGAGUGACAUGCACCAGGACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3910-1 MI0016414 (SEQ ID NO: 1769) 
                   
               
               
                 CUUUUGCUGUCAGUUUUUCUGUUGCUUGUCUUGGUUUUAUGCCUUUUAUAUCAAGGCACAUAAAAGGCAUAAAACCA 
                   
               
               
                 AGACAAGCAACAAAAAAAGGAUUGAUCACAGAAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3910-2 MI0016431 (SEQ ID NO: 1770) 
                   
               
               
                 UUUUUUUGUUGCUUGUCUUGGUUUUAUGCCUUUUAUGUGCCUUGAUAUAAAAGGCAUAAAACCAAGACAAGCAACAG 
                   
               
               
                 AAAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3911 MI0016415 (SEQ ID NO: 1771) 
                   
               
               
                 GGGUGAGGAUGUGUGUGGAUCCUGGAGGAGGCAGAGAAGACAGUGAGCUUGCCAGUUCUGGUUUCCAACACUUCCUU 
                   
               
               
                 UCCUGCGCUUCUCGAUUCCCAGAUCUGCACCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3912 MI0016416 (SEQ ID NO: 1772) 
                   
               
               
                 AGAGAGGAAUGAACAGUUAAAUUAUAACAUGUCCAUAUUAUGGGUUAGUUGUGGACACAUACUAACGCAUAAUAUGG 
                   
               
               
                 ACAUGUUAUAAUUUAACUGUUCCUUUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3913-1 MI0016417 (SEQ ID NO: 1773) 
                   
               
               
                 UUGUUUAUAAUAAACUGAAAUAUUUGGGACUGAUCUUGAUGUCUGCCAAAACCUUGGCAGACAUCAAGAUCAGUCCC 
                   
               
               
                 AAAUAUUUCAGUUUAUUAUAGACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3913-2 MI0016418 (SEQ ID NO: 1774) 
                   
               
               
                 UGUCUAUAAUAAACUGAAAUAUUUGGGACUGAUCUUGAUGUCUGCCAAGGUUUUGGCAGACAUCAAGAUCAGUCCCA 
                   
               
               
                 AAUAUUUCAGUUUAUUAUAAACA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3914-1 MI0016419 (SEQ ID NO: 1775) 
                   
               
               
                 UGGACUUCAGAUUUAACUUCUCAUUUUCUGGUUCCUUCUAAUGAGUAUGCUUAACUUGGUAGAAGGAACCAGAAAAU 
                   
               
               
                 GAGAAGUUGAGUAGGAACUCUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3914-2 MI0016421 (SEQ ID NO: 1776) 
                   
               
               
                 GAGUUCCUACUCAACUUCUCAUUUUCUGGUUCCUUCUACCAAGUUAAGCAUACUCAUUAGAAGGAACCAGAAAAUGA 
                   
               
               
                 GAAGUUAAAUCUGAAGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3915 MI0016420 (SEQ ID NO: 1777) 
                   
               
               
                 CAAGUUGGCACUGUAGAAUAUUGAGGAAAAGAUGGUCUUAUUGCAAAGAUUUUCAAUAAGACCAUCCUUUCCUCAAU 
                   
               
               
                 AUUCUGUGGUGUCAUCUUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3916 MI0016422 (SEQ ID NO: 1778) 
                   
               
               
                 AUCCCAGAGAAGAAGGAAGAAGAGGAAGAAAUGGCUGGUUCUCAGGUGAAUGUGUCUGGGUUCAGGGGAUGUGUCUC 
                   
               
               
                 CUCUUUUCUUCUGGGAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3917 MI0016423 (SEQ ID NO: 1779) 
                   
               
               
                 GGCGCUUUUGUGCGCGCCCGGGUCUGUUGGUGCUCAGAGUGUGGUCAGGCGGCUCGGACUGAGCAGGUGGGUGCGGG 
                   
               
               
                 GCUCGGAGGAGGCGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3918 MI0016424 (SEQ ID NO: 1780) 
                   
               
               
                 AGGCGGUUAAGCCAUGGGACAGGGCCGCAGAUGGAGACUGCUCAAGGUCAAAGGGGUCUCCAGCUGGGACCCUGCAC 
                   
               
               
                 CUGGUUCGUAGCCCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3919 MI0016425 (SEQ ID NO: 1781) 
                   
               
               
                 CCUGAGCACCAUUUACUGAGUCCUUUGUUCUCUACUAGUUUGUAGUAGUUCGUAGCAGAGAACAAAGGACUCAGUAA 
                   
               
               
                 AUGGUGCUCAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3920 MI0016427 (SEQ ID NO: 1782) 
                   
               
               
                 ACUGAGUGAGGGAGUCAGAGAGUUAAGAGAAUUAGUACAGGUGAGAUUGUACUGAUUAUCUUAACUCUCUGACCCCC 
                   
               
               
                 UCACUCAGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3921 MI0016428 (SEQ ID NO: 1783) 
                   
               
               
                 CCUAGCCCAGUACAAGGCAUAUGGUACUCAAGAGACUUAGAAAUCCCUAAGUCUCUGAGUACCAUAUGCCUUGUACU 
                   
               
               
                 GGGCUAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3922 MI0016429 (SEQ ID NO: 1784) 
                   
               
               
                 GGAAGAGUCAAGUCAAGGCCAGAGGUCCCACAGCAGGGCUGGAAAGCACACCUGUGGGACUUCUGGCCUUGACUUGA 
                   
               
               
                 CUCUUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3923 MI0016430 (SEQ ID NO: 1785) 
                   
               
               
                 GGUAGAGUGAGCUCUAAUCCAAUAUUACUAGCUUCUUUAUAAGAAGAGGAAACUAGUAAUGUUGGAUUAGGGCUCAC 
                   
               
               
                 UCUACU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3924 MI0016432 (SEQ ID NO: 1786) 
                   
               
               
                 UAAAUGAAAAAGUAGUAGUCAAAUAUGCAGAUCUAUGUCAUAUAUACAGAUAUGUAUAUGUGACUGCUACUUUUUUG 
                   
               
               
                 UUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3925 MI0016433 (SEQ ID NO: 1787) 
                   
               
               
                 GUGGGAAUAGCAAGAGAACUGAAAGUGGAGCCUGUCACAUCUCCAGACUCCAGUUUUAGUUCUCUUGCUAUUUCCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3926-1 MI0016434 (SEQ ID NO: 1788) 
                   
               
               
                 AAAAUGGAGCUGGCCAAAAAGCAGGCAGAGACUUUAAAAGCGUCUCUGCCUGCUUUUUGGCCAGCUCCGUUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3926-2 MI0016437 (SEQ ID NO: 1789) 
                   
               
               
                 GGAGCUGGCCAAAAAGCAGGCAGAGACGCUUUUAAAGUCUCUGCCUGCUUUUUGGCCAGCUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3927 MI0016435 (SEQ ID NO: 1790) 
                   
               
               
                 UGCCAAUGCCUAUCACAUAUCUGCCUGUCCUAUGACAAACAUGGCAGGUAGAUAUUUGAUAGGCAUUGGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3928 MI0016438 (SEQ ID NO: 1791) 
                   
               
               
                 GCUGAAGCUCUAAGGUUCCGCCUGCGGGCAGGAAGCGGAGGAACCUUGGAGCUUCGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3929 MI0016439 (SEQ ID NO: 1792) 
                   
               
               
                 AGUGGCUCACACCAGUAAUCCCAGCACUUUGGGAGGCUGAUGUGAGUAGACCACU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3934 MI0016590 (SEQ ID NO: 1793) 
                   
               
               
                 CACAGCCCUUCCUGUCCCCAGUUUUCAGGUGUGGAAACUGAGGCAGGAGGCAGUGAAGUAACUUGCUCAGGUUGCAC 
                   
               
               
                 AGCUGGGAAGUGGAGCAGGGAUUUGAAUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3935 MI0016591 (SEQ ID NO: 1794) 
                   
               
               
                 GGAUGUGUUCCUGUCCCAGAAGGAGCUGAUGGUUGUAUCUAUGAAGGUAAGCAUUUUUGUAGAUACGAGCACCAGCC 
                   
               
               
                 ACCCUAAGCAAAGGCAGAGAAUGCUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3936 MI0016592 (SEQ ID NO: 1795) 
                   
               
               
                 AUGAUUCAGAGCAUCUGUCCAGUGUCUGCUGUAGAUCCCUCAAAUCCGUGUUUGGACGCUUCUGGUAAGGGGUGUAU 
                   
               
               
                 GGCAGAUGCACCCGACAGAUGCACUUGGCAGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3937 MI0016593 (SEQ ID NO: 1796) 
                   
               
               
                 AGAAGAAUGCCCAACCAGCCCUCAGUUGCUACAGUUCCCUGUUGUUUCAGCUCGACAACAACAGGCGGCUGUAGCAA 
                   
               
               
                 UGGGGGGCUGGAUGGGCAUCUCAAUGUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3938 MI0016594 (SEQ ID NO: 1797) 
                   
               
               
                 AGGAAUUUUUAACCCGAUCACUAGAUUAUCUACAAGGGAAUUUUUUUUUAAUUUAAAAAAUUCCCUUGUAGAUAACC 
                   
               
               
                 CGGUGGUCAGGUUGGAUGGCUCCAUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3939 MI0016596 (SEQ ID NO: 1798) 
                   
               
               
                 CUGGCUUCCAAAGGCCUCUGUGUGUUCCUGUAUGUGGGCGUGCACGUACCUGUCACAUGUGUACGCGCAGACCACAG 
                   
               
               
                 GAUGUCCACACUGGCUUCCAAACACAUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3940 MI0016597 (SEQ ID NO: 1799) 
                   
               
               
                 GCUUAUCGAGGAAAAGAUCGAGGUGGGUUGGGGCGGGCUCUGGGGAUUUGGUCUCACAGCCCGGAUCCCAGCCCACU 
                   
               
               
                 UACCUUGGUUACUCUCCUUCCUUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3941 MI0016598 (SEQ ID NO: 1800) 
                   
               
               
                 GAGUCAGAAUUCUCAUCAGGCUGUGAUGCUCAGUUGUGUGUAGAUUGAAAGCCCUAAUUUUACACACAACUGAGGAU 
                   
               
               
                 CAUAGCCUGAUGGUUCCUUUUUGUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3942 MI0016599 (SEQ ID NO: 1801) 
                   
               
               
                 UCUUCAGUAUGACACCUCAAAGAAGCAAUACUGUUACCUGAAAUAGGCUGCGAAGAUAACAGUAUUUCAGAUAACAG 
                   
               
               
                 UAUUACAUCUUUGAAGUGUCAUAUUCACUGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3943 MI0016600 (SEQ ID NO: 1802) 
                   
               
               
                 CACACAGACGGCAGCUGCGGCCUAGCCCCCAGGCUUCACUUGGCGUGGACAACUUGCUAAGUAAAGUGGGGGGUGGG 
                   
               
               
                 CCACGGCUGGCUCCUACCUGGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3944 MI0016601 (SEQ ID NO: 1803) 
                   
               
               
                 UCCACCCAGCAGGCGCAGGUCCUGUGCAGCAGGCCAACCGAGAAGCGCCUGCGUCUCCCAUUUUCGGGCUGGCCUGC 
                   
               
               
                 UGCUCCGGACCUGUGCCUGAUCUUAAUGCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3945 MI0016602 (SEQ ID NO: 1804) 
                   
               
               
                 GAUGUUGAUGCACGUGACGGGGAGGGCAUAGGAGAGGGUUGAUAUAAAAUGCAAUUACAGCCUCUUAUGCUUUCCAA 
                   
               
               
                 AGUGGGGGAUGAUUCAAUGAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3960 MI0016964 (SEQ ID NO: 1805) 
                   
               
               
                 GGCGCCCCGGCUCCCCGCGCCCCCGAUCGGGGCCGCCGCUAGUAGUGGCGGCGGCGGAGGCGGGGGCAGCGGCGGCG 
                   
               
               
                 GCGGCGGAGGCGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3972 MI0016990 (SEQ ID NO: 1806) 
                   
               
               
                 GCCCAUUUGCCUUGGCUUGGGGUGGCAGUCCUGUGGGAAUGAGAGAUGCCAAACUGGACCUGCCAGCCCCGUUCCAG 
                   
               
               
                 GGCACAGCAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3973 MI0016991 (SEQ ID NO: 1807) 
                   
               
               
                 GCCCAGGGUAGCUCUCUGUAUUGCUUGUUACAUUUUAGGAUUGCUUGCCCCUUGCUCCAAUGGUGCAGGCAGAAGAA 
                   
               
               
                 AUGCAAACAAAGUACAGCAUUAGCCUUAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3974 MI0016992 (SEQ ID NO: 1808) 
                   
               
               
                 GUUCAGGGAAAAGGUCAUUGUAAGGUUAAUGCACCCAUAUUUUAAUAUCAAACUAUGACAAAUUUGACUACAGCCUU 
                   
               
               
                 UCCGUACCCCUGCCAAAAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3975 MI0016993 (SEQ ID NO: 1809) 
                   
               
               
                 CAUGUAGGUGAGUGAUUGCUAUUUCAAAAGACUAGAGGUAGGAAAUGAGGCUAAUGCACUACUUCACAUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3976 MI0016994 (SEQ ID NO: 1810) 
                   
               
               
                 UGAGGGAUAUAGAGAGCAGGAAGAUUAAUGUCAUAUUGGAGUUGGACUGCAGGGCUUCCUUUACACAAUAAAUAUUG 
                   
               
               
                 UAUGAAGUGCUGAUGUAACCUUUACUGCAGCAUGACAUGGGAUUUGGCUGUUUUUAUGGCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3977 MI0016995 (SEQ ID NO: 1811) 
                   
               
               
                 UUGUGCUUCAUCGUAAUUAACCUUAAGUGGUUCGGGUAAAUCACUUUAAUUUGUUAUGUGUUGGCAGAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-3978 MI0016996 (SEQ ID NO: 1812) 
                   
               
               
                 UCAGUGGAAAGCAUGCAUCCAGGGUGUGGAGCCAAAAUUAGAAGGGCCAAAAUUCUACCUGGCCCACUACCACAGCA 
                   
               
               
                 ACCUUGGGCAUCGUUUUCUUUUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4251 MI0015861 (SEQ ID NO: 1813) 
                   
               
               
                 CACGUCCUCCAGCUUUUUUCCUUAGUGGCCAAUUCCUGAGAAAAGGGCCAACGUGCUUCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4252 MI0015864 (SEQ ID NO: 1814) 
                   
               
               
                 UGGGGGGCUGGCAGCUCAUCAGUCCAGGCCAUCUGGCCACUGAGUCAGCACCAGCGCCCAAUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4253 MI0015860 (SEQ ID NO: 1815) 
                   
               
               
                 CCAGCCAUCGCCCUUGAGGGGCCCUAGGACUUACUUGUGCAGGGCAUGUCCAGGGGGUCCAGGUCUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4254 MI0015862 (SEQ ID NO: 1816) 
                   
               
               
                 CUUGGGAGGAGGGUGGGGUGGCUCCUCUGCAGUGAGUAGGUCUGCCUGGAGCUACUCCACCAUCUCCCCCAGCCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4255 MI0015863 (SEQ ID NO: 1817) 
                   
               
               
                 GAGCAUCCUUCAGUGUUCAGAGAUGGAGUCAGUAUUGGUCUGGCCAUUUUUAGGGCAAAGAGGCAGCAUCAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4256 MI0015855 (SEQ ID NO: 1818) 
                   
               
               
                 UGUUCCAUUUAUCUGACCUGAUGAAGGUCUCCUGGCAUUGAUUAGGUCUGAUGAUCCAUUUCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4257 MI0015856 (SEQ ID NO: 1819) 
                   
               
               
                 GGCUUAGAAACAGUCCCUAGGUAGGAUUUGGGGAGGAGCUAAGAAGCCCCUACAGGGCCCAGAGGUGGGGACUGAGC 
                   
               
               
                 CUUAGUUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4258 MI0015857 (SEQ ID NO: 1820) 
                   
               
               
                 ACGCCCCCCGCCCCGCCACCGCCUUGGAGGCUGACCUCUUACUUUCGGUCGGUCUUCUUCCCUGGGCUUGGUUUGGG 
                   
               
               
                 GGCGGGGGAGUGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4259 MI0015858 (SEQ ID NO: 1821) 
                   
               
               
                 GAUGGGCCCCUUGUGUCCUGAAUUGGGUGGGGGCUCUGAGUGGGGAAAGUGGGGGCCUAGGGGAGGUCACAGUUGGG 
                   
               
               
                 UCUAGGGGUCAGGAGGGCCCAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4260 MI0015859 (SEQ ID NO: 1822) 
                   
               
               
                 AACAAGGUGACUUGGGGCAUGGAGUCCCACUUCCUGGAGCCCACACCCCAGCUUGUCACACACCAAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4261 MI0015868 (SEQ ID NO: 1823) 
                   
               
               
                 GGUGGAAGUGGGUUCCUCCCAGUUCCUGAGACAGGAAACAGGGACCCAGGAGACCAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4262 MI0015872 (SEQ ID NO: 1824) 
                   
               
               
                 GAAAGCUGCAGGUGCUGAUGUUGGGGGGACAUUCAGACUACCUGCAGCAGAGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4263 MI0015876 (SEQ ID NO: 1825) 
                   
               
               
                 AUAGUGCUCUUCAGGGUUUUACUUGGGAGAUUGGAGUGGCCAGUGUUCCUAAACAAUUCUAAGUGCCUUGGCCCACA 
                   
               
               
                 ACAUAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4264 MI0015877 (SEQ ID NO: 1826) 
                   
               
               
                 AAAGCUGGAUACUCAGUCAUGGUCAUUGUAACAUGAUAGUGACAGGUACUGGGUAAGACUGCAUAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4265 MI0015869 (SEQ ID NO: 1827) 
                   
               
               
                 UGCAGUGGGUUGGAGCUUCAGCCUACACCUGUAAAGAAUUGGUCAGCCUGGGGACUGGUGAUCUCUGCAGCUGUGGG 
                   
               
               
                 CUCAGCUCUGGGCUGGGCCUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4266 MI0015870 (SEQ ID NO: 1828) 
                   
               
               
                 CCACUGCUGGCCGGGGCCCCUACUCAAGGCUAGGAGGCCUUGGCCAAGGACAGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4267 MI0015871 (SEQ ID NO: 1829) 
                   
               
               
                 CUCAGCAGGCUCCAGCUCGGUGGCACUGGGGGAAGGCUCCAGACCCCAGCCUCUGUCAUCCCUGCAUGGAGCCCACA 
                   
               
               
                 UCUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4268 MI0015874 (SEQ ID NO: 1830) 
                   
               
               
                 AUGCACAUCAGGUUCUAGAGGUUUUGCCCUAGCGGCUCCUCCUCUCAGGAUGUGAUGUCACCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4269 MI0015875 (SEQ ID NO: 1831) 
                   
               
               
                 ACAGCGCCCUGCAGGCACAGACAGCCCUGGCUUCUGCCUCUUUCUUUGUGGAAGCCACUCUGUCAGGCCUGGGAUGG 
                   
               
               
                 AGGGGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4270 MI0015878 (SEQ ID NO: 1832) 
                   
               
               
                 ACAAAUAGCUUCAGGGAGUCAGGGGAGGGCAGAAAUAGAUGGCCUUCCCCUGCUGGGAAGAAAGUGGGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4271 MI0015879 (SEQ ID NO: 1833) 
                   
               
               
                 AAAUCUCUCUCCAUAUCUUUCCUGCAGCCCCCAGGUGGGGGGGAAGAAAAGGUGGGGAAUUAGAUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4272 MI0015880 (SEQ ID NO: 1834) 
                   
               
               
                 UUUUCUGCACAAAUUAAUCAGUUAAUGCAUAGAAAGCAUUCAACUAGUGAUUGUGUUAUAAGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4273 MI0015881 (SEQ ID NO: 1835) 
                   
               
               
                 UCCCCUGUGUGUGUUCUCUGAUGGACAGUAAGCCUUGACUUAUGGCUAAAUGCUUCUUCACAAUGGUCACAUGCAUA 
                   
               
               
                 GGGCUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4274 MI0015884 (SEQ ID NO: 1836) 
                   
               
               
                 GGGGCAUUUAGGGUAACUGAGCUGCUGCCGGGGCCUGGCGCUCCUCUACCUUGUCAGGUGACCCAGCAGUCCCUCCC 
                   
               
               
                 CCUGCAUGGUGCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4275 MI0015883 (SEQ ID NO: 1837) 
                   
               
               
                 ACAUUUUUGUCCAAUUACCACUUCUUUUUGCCACCUGAGCACAGUCAGCAGUCAGCAUAAAAAAGUGAUAAUGGGAA 
                   
               
               
                 GUUAAUGUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4276 MI0015882 (SEQ ID NO: 1838) 
                   
               
               
                 CACAGUCUGACUCAGUGACUCAUGUGCUGGCAGUGGCCACGUAAAUAGAGCUACUGUGUCUGAAAGCAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4277 MI0015886 (SEQ ID NO: 1839) 
                   
               
               
                 CUGGGUCGAGGCAGUUCUGAGCACAGUACACUGGGCUGCCCCCACUGCCCAGUGCCCUGCUCAGCUCAAGUCCUUGU 
                   
               
               
                 GCCCCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4278 MI0015888 (SEQ ID NO: 1840) 
                   
               
               
                 AUCUAACACCAGGAGAAUCCCAUAGAACAUUGACAUCAACACUAGGGGGUUUGCCCUUGUGGGGAAGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4279 MI0015887 (SEQ ID NO: 1841) 
                   
               
               
                 UGCUCUGUGGAGCUGAGGAGCAGAUUCUCUCUCUCUCCUCCCGGCUUCACCUCCUGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4280 MI0015889 (SEQ ID NO: 1842) 
                   
               
               
                 AAUCAGGGUGGAGUGUAGUUCUGAGCAGAGCCUUAAAGGAUGAGGUAUGUUCAAGACUGAAUGACACCUUUGUGAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4281 MI0015885 (SEQ ID NO: 1843) 
                   
               
               
                 GCUGGGGGUCCCCCGACAGUGUGGAGCUGGGGCCGGGUCCCGGGGAGGGGGGUUCUGGGCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4282 MI0015890 (SEQ ID NO: 1844) 
                   
               
               
                 GGUGAAGUUCCAGGGGAAGAUUUUAGUAUGCCACAUUUCUAAAAUUUGCAUCCAGGAACAUCAUCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4283-1 MI0015892 (SEQ ID NO: 1845) 
                   
               
               
                 ACUCUGAUCCUGGGGCUCAGCGAGUUUGCAAGGGGUGUUUCUGUCCAUGGUCAGGCUUGCCAGCCUUGGUCCUUGGG 
                   
               
               
                 CCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4283-2 MI0015982 (SEQ ID NO: 1846) 
                   
               
               
                 ACUCUGAUCCUGGGGCUCAGCGAGUUUGCAAGGGGUGUUUCUGUCCAUGGUCAGGCUUGCCAGCCUUGGUCCUUGGG 
                   
               
               
                 CCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4284 MI0015893 (SEQ ID NO: 1847) 
                   
               
               
                 GUUCUGUGAGGGGCUCACAUCACCCCAUCAAAGUGGGGACUCAUGGGGAGAGGGGGUAGUUAGGAGCUUUGAUAGAG 
                   
               
               
                 GCGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4285 MI0015891 (SEQ ID NO: 1848) 
                   
               
               
                 AUUAGCUGGGGCGGCGAGUCCGACUCAUCAAUAUUUUAAGGAAUGACCCGGCCUUGGGGUGCGGAAUUGCUGCGCGG 
                   
               
               
                 GCGGGGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4286 MI0015894 (SEQ ID NO: 1849) 
                   
               
               
                 UACUUAUGGCACCCCACUCCUGGUACCAUAGUCAUAAGUUAGGAGAUGUUAGAGCUGUGAGUACCAUGACUUAAGUG 
                   
               
               
                 UGGUGGCUUAAACAUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4287 MI0015895 (SEQ ID NO: 1850) 
                   
               
               
                 UAGUUCUUUUUCUCCCUUGAGGGCACUUUUCAGUUCCUGAGAUCAAUGUGGUCCCUACUGGGGAGACCAUAGGAGCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4288 MI0015896 (SEQ ID NO: 1851) 
                   
               
               
                 AUGGAGGUGGAGAGUCAUCAGCAGCACUGAGCAGGCAGUGUUGUCUGCUGAGUUUCCACGUCAUUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4289 MI0015898 (SEQ ID NO: 1852) 
                   
               
               
                 CCUUGGGAGGGCAUUGUGCAGGGCUAUCAGGCAGUUUCCUGGGCCCUGUCUGCAGAGCCUAAACAGAUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4290 MI0015899 (SEQ ID NO: 1853) 
                   
               
               
                 GCCACCAAGAAGGUGAAGGGAGGGUCAGUCCCAAUCUGAAUCCCACCAAAAUAGGUGGUAGAGGGUUGCCCUCCUUU 
                   
               
               
                 CUUCCCUCACCUCUGACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4291 MI0015900 (SEQ ID NO: 1854) 
                   
               
               
                 CGCCGGGGGCUUCAGCAGGAACAGCUGGGUGGAGGCAGAGCUGUUCUGCUGUGGCUGCAGCCCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4292 MI0015897 (SEQ ID NO: 1855) 
                   
               
               
                 GAGACACCAGAAGGCCACCUGCUUAGGAGGCCAGAGGUGCCCCUGGGCCGGCCUUGGUGAGGGGCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4293 MI0015826 (SEQ ID NO: 1856) 
                   
               
               
                 AGAGACACCUGUUCCUUGGGAAGCUGGUGACAUUGCUAAUUCAUUUCACACCAGCCUGACAGGAACAGCCUGACUGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4294 MI0015827 (SEQ ID NO: 1857) 
                   
               
               
                 CCGAUGCCUCGGGAGUCUACAGCAGGGCCAUGUCUGUGAGGGCCCAAGGGUGCAUGUGUCUCCCAGGUUUCGGUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4295 MI0015822 (SEQ ID NO: 1858) 
                   
               
               
                 CUUUGUGGAACAGUGCAAUGUUUUCCUUGCCUGUGGCAAGACCACUUCGGUUCAAGGCUAAGAAACUAGACUGUUCC 
                   
               
               
                 UACAGAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4296 MI0015823 (SEQ ID NO: 1859) 
                   
               
               
                 UUGGGCUUUGAUGUGGGCUCAGGCUCAGAGGGCUGAAGUGGUUGUGGGGAGGGGCUUCUGGGGACUGUGUCCAUGUC 
                   
               
               
                 UCUGUCGUUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4297 MI0015824 (SEQ ID NO: 1860) 
                   
               
               
                 AGCACGCACGUGCCUUCCUGUCUGUGCCUGCCUUCGAAGUGCACGGCAGGGCCAGGACGGGUCGCUGUGGGUGGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4298 MI0015830 (SEQ ID NO: 1861) 
                   
               
               
                 GGGGAGGUACCUGGGACAGGAGGAGGAGGCAGCCUUGCCUCAGAAACCAAACUGUCAAAAGUGUAGGUUCCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4299 MI0015829 (SEQ ID NO: 1862) 
                   
               
               
                 GGGUUCUGACCAAUCAUGUUACAGUGUUUUCUCCUUUAGAGAGAGCUGGUGACAUGAGAGGCAGAAAAAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4300 MI0015831 (SEQ ID NO: 1863) 
                   
               
               
                 UGAGUUUAGAAGAGGGCCAGCUAAAUCAGCAGAGACAUGAGGUGAUCAAAAACCUUUUUUCAAAGCAGUGGGAGCUG 
                   
               
               
                 GACUACUUCUGAACCAAUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4301 MI0015828 (SEQ ID NO: 1864) 
                   
               
               
                 ACCAGCCACCUCCCACUACUUCACUUGUGAACAUUGCAUUCGUGGAGGGUGGCAGGUGCAGCUCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4302 MI0015833 (SEQ ID NO: 1865) 
                   
               
               
                 UCAGGAGGGACCAGUGUGGCUCAGCGAGGUGGCUGAGUUUACUUAAGGUAUUGGAAUGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4303 MI0015834 (SEQ ID NO: 1866) 
                   
               
               
                 AGAAAAUAGCUUCUGAGCUGAGGACAGCUUGCUCUGCUUUUCUUUAGCUUAGGAGCUAACCAUGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4304 MI0015832 (SEQ ID NO: 1867) 
                   
               
               
                 AGAGAAGUGGCCGGCAUGUCCAGGGCAUCCCCAUUGCUCUGUGACUGCUGCCAUCCUUCUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4305 MI0015835 (SEQ ID NO: 1868) 
                   
               
               
                 CUGCCUUAGACCUAGACACCUCCAGUUCUGGGUUCUUAGAGGCCUAAUCCUCUACAAACUCAGUUUUCAGACUGUGA 
                   
               
               
                 GGGAAAUUCUCUGUCUUAUUGCUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4306 MI0015836 (SEQ ID NO: 1869) 
                   
               
               
                 AAGCUGCUUAGUGUCCUUAGAGUCUCCAGAGGCAUCCCUAACCCAGAAUCUUUUGACUGUCCUCUGGAGAGAAAGGC 
                   
               
               
                 AGUAGGUCUGUACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4307 MI0015838 (SEQ ID NO: 1870) 
                   
               
               
                 UCAGAAGAAAAAACAGGAGAUAAAGUUUGUGAUAAUGUUUGUCUAUAUAGUUAUGAAUGUUUUUUCCUGUUUCCUUC 
                   
               
               
                 AGGGCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4308 MI0015839 (SEQ ID NO: 1871) 
                   
               
               
                 UAUGGGUUCAGAGGGAACUCCAUUGGACAGAAAUUUCCUUUUGAGGAAAUCUUUCCCUGGAGUUUCUUCUUACCUUU 
                   
               
               
                 UUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4309 MI0015837 (SEQ ID NO: 1872) 
                   
               
               
                 UCUGGGGGUUCUGGAGUCUAGGAUUCCAGGAUCUGGGUUUUGAGGUCUUGGGUUGUAGGGUCUGCGGUUUGAAGCCC 
                   
               
               
                 CUCUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4310 MI0015840 (SEQ ID NO: 1873) 
                   
               
               
                 UGGCGUCUGGGGCCUGAGGCUGCAGAACAUUGCAGCAUUCAUGUCCCACCCCCACCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4311 MI0015841 (SEQ ID NO: 1874) 
                   
               
               
                 UCAGAGAGGGGAAAGAGAGCUGAGUGUGACCUGGAGCAGCUCAGGAGGGCUUCCUGGGUGAGGUGGCAGGUUACAGG 
                   
               
               
                 UUCGAUCUUUGGCCCUCAGAUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4312 MI0015842 (SEQ ID NO: 1875) 
                   
               
               
                 GAAAGGUUGGGGGCACAGAGAGCAAGGAGCCUUCCCCAGAGGAGUCAGGCCUUGUUCCUGUCCCCAUUCCUCAGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4313 MI0015843 (SEQ ID NO: 1876) 
                   
               
               
                 GAUCAGGCCCAGCCCCCUGGCCCCAAACCCUGCAGCCCCAGCUGGAGGAUGAGGAGAUGCUGGGCUUGGGUGGGGGA 
                   
               
               
                 AUCAGGGGUGUAAAGGGGCCUGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4314 MI0015846 (SEQ ID NO: 1877) 
                   
               
               
                 GGCCAUUCCUCUCUGGGAAAUGGGACAGGUAGUGGCCACAGUGAGAAAGCUGGCCUGUCCUUCUGCCCCAGGGCCCA 
                   
               
               
                 GAGUCUGUGACUGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4315-1 MI0015844 (SEQ ID NO: 1878) 
                   
               
               
                 UGGGCUUUGCCCGCUUUCUGAGCUGGACCCUCUCUCUACCUCUGGUGCAGAACUACAGCGGAAGGAAUCUCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4315-2 MI0015983 (SEQ ID NO: 1879) 
                   
               
               
                 UGGGCUUUGCCCGCUUUCUGAGCUGGACCCUCUCUCUACCUCUGGUGCAGAACUACAGCGGAAGGAAUCUCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4316 MI0015845 (SEQ ID NO: 1880) 
                   
               
               
                 AGUGGCCCAGGGUGAGGCUAGCUGGUGUGGUCACCCACUCUCCAGCCCAGCCCCAAUCCCACCACAACCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4317 MI0015850 (SEQ ID NO: 1881) 
                   
               
               
                 AAAAGGCGAGACAUUGCCAGGGAGUUUAUUUUGUAGCUCUCUUGAUAAAAUGUUUUAGCAAACAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4318 MI0015847 (SEQ ID NO: 1882) 
                   
               
               
                 GCUUCUUAAUUAUGUCAUAAACCCACUGUGGACAAGGGCCUUGUCUUAGACAGUCACUGUGGGUACAUGCUAGGUGC 
                   
               
               
                 UCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4319 MI0015848 (SEQ ID NO: 1883) 
                   
               
               
                 UUGGCUUGAGUCCCUGAGCAAAGCCACUGGGAAUGCUCCCUGAGGACGUUAUAUGAGUGCUCAGCUCAUGGGGCUAU 
                   
               
               
                 GAUGGUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4320 MI0015849 (SEQ ID NO: 1884) 
                   
               
               
                 GACAUGUGGGGUUUGCUGUAGACAUUUCAGAUAACUCGGGAUUCUGUAGCUUCCUGGCAACUUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4321 MI0015852 (SEQ ID NO: 1885) 
                   
               
               
                 CUGGUCUCCGCAGAGCCUCUGCCCCUCCCGAGACACCCGCUACCUGGUGUUAGCGGUGGACCGCCCUGCGGGGGCCU 
                   
               
               
                 GGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4322 MI0015851 (SEQ ID NO: 1886) 
                   
               
               
                 ACCGCGAGUUCCGCGCCUGGCCGUGUCGCCCCACGAGGGGGACUGUGGGCUCAGCGCGUGGGGCCCGGAGCAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4323 MI0015853 (SEQ ID NO: 1887) 
                   
               
               
                 CGGGGCCCAGGCGGGCAUGUGGGGUGUCUGGAGACGCCAGGCAGCCCCACAGCCUCAGACCUCGGGCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4324 MI0015854 (SEQ ID NO: 1888) 
                   
               
               
                 CGGCCCCUUUGUUAAGGGUCUCAGCUCCAGGGAACUUUAAAACCCUGAGACCCUAACCUUAAAGGUGCUGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4325 MI0015865 (SEQ ID NO: 1889) 
                   
               
               
                 GGGGAAGAUGUUGCACUUGUCUCAGUGAGAGAUGCUUCUAGAUCCAGGAGGCAGACCUCAAGGAUGGAGAGAAGGCA 
                   
               
               
                 GAUCCUUUGAGAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4326 MI0015866 (SEQ ID NO: 1890) 
                   
               
               
                 GCUGCUCUGCUGUUCCUCUGUCUCCCAGACUCUGGGUGGAUGGAGCAGGUCGGGGGCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4327 MI0015867 (SEQ ID NO: 1891) 
                   
               
               
                 GGCCUGGGUAGGCUUGCAUGGGGGACUGGGAAGAGACCAUGAACAGGUUAGUCCAGGGAGUUCUCAUCAAGCCUUUA 
                   
               
               
                 CUCAGUAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4328 MI0015904 (SEQ ID NO: 1892) 
                   
               
               
                 AACAGUUGAGUCCUGAGAACCAUUGAGAACCAGUUUUCCCAGGAUUAACUGUUCCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4329 MI0015901 (SEQ ID NO: 1893) 
                   
               
               
                 UAGAGAGGAAGGUGUACCAGGGUUUUGGAGUUUUUUUUUCCUCCUGAGACCCUAGUUCCACAUUCUGGAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4330 MI0015902 (SEQ ID NO: 1894) 
                   
               
               
                 AAUUGUCAGCAGGCAAUUAUCUGAGGAUGCAGGAGAGGAAGGGGGCUUCUUUUUGACGCCUACUUCAUCAGCUGCUC 
                   
               
               
                 CUCAGAUCAGAGCCUUGCAGGUCAGGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4417 MI0016753 (SEQ ID NO: 1895) 
                   
               
               
                 GAAAACAACCAGGUGGGCUUCCCGGAGGGCGGAACACCCAGCCCCAGCAUCCAGGGCUCACCUACCACGUUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4418 MI0016754 (SEQ ID NO: 1896) 
                   
               
               
                 UGGUUUUUGCUCUGAGUGACCGUGGUGGUUGUGGGAGUCACUGCAGGACUCAGCAGGAAUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4419a MI0016755 (SEQ ID NO: 1897) 
                   
               
               
                 UGGUGGUGUGUGCCUGUAGUCUUAGCUACUCGGGAGGCUGAGGGAGGAGACUGCAGUGAGUGGAGGUCACGCCACUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4419b MI0016861 (SEQ ID NO: 1898) 
                   
               
               
                 CUCAGGCUCAGUGGUGCAUGCUUAUAGUCCCAGCCACUCUGGAGGCUGAAGGAAGAUGGCUUGAGCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4420 MI0016757 (SEQ ID NO: 1899) 
                   
               
               
                 CUCUUGGUAUGAACAUCUGUGUGUUCAUGUCUCUCUGUGCACAGGGGACGAGAGUCACUGAUGUCUGUAGCUGAGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4421 MI0016758 (SEQ ID NO: 1900) 
                   
               
               
                 CUGGGUCUCCUUUCUGCUGAGAGUUGAACACUUGUUGGGACAACCUGUCUGUGGAAAGGAGCUACCUAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4422 MI0016759 (SEQ ID NO: 1901) 
                   
               
               
                 AGUUCUUCUGCAGACAAAAGCAUCAGGAAGUACCCACCAUGUACCAGUGGGCCCUUCUUGAUGCUCUUGAUUGCAGA 
                   
               
               
                 GGAGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4423 MI0016760 (SEQ ID NO: 1902) 
                   
               
               
                 AUCAUGUACUGCAGUUGCCUUUUUGUUCCCAUGCUGUUUAAGCCUAGCAUAGGCACCAAAAAGCAACAACAGUAUGU 
                   
               
               
                 GAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4424 MI0016763 (SEQ ID NO: 1903) 
                   
               
               
                 CUUACAUCACACACAGAGUUAACUCAAAAUGGACUAAUUUUUCCACUAGUUAGUCCAUUUCAAGUUAACUCUGUGUG 
                   
               
               
                 UGAUGUAGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4425 MI0016764 (SEQ ID NO: 1904) 
                   
               
               
                 GUGCUUUACAUGAAUGGUCCCAUUGAAUCCCAACAGCUUUGCGAAGUGUUGUUGGGAUUCAGCAGGACCAUUCGUGU 
                   
               
               
                 AAAGUAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4426 MI0016765 (SEQ ID NO: 1905) 
                   
               
               
                 AGUUGGAAGAUGGACGUACUUUGUCUGACUACAAUAUUCAAAAGGAGUCUACUCUUCAUCUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4427 MI0016766 (SEQ ID NO: 1906) 
                   
               
               
                 GAAGCCUCUUGGGGCUUAUUUAGACAAUGGUUUCAUCAUUUCGUCUGAAUAGAGUCUGAAGAGUCUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4428 MI0016767 (SEQ ID NO: 1907) 
                   
               
               
                 UUGGCAGGUGCCAUGUUGCCUGCUCCUUACUGUACACGUGGCUGGCAAGGAGACGGGAACAUGGAGCCGCCAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4429 MI0016768 (SEQ ID NO: 1908) 
                   
               
               
                 AGGGAGAAAAGCUGGGCUGAGAGGCGACUGGUGUCUAAUUUGUUUGUCUCUCCAACUCAGACUGCCUGGCCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4430 MI0016769 (SEQ ID NO: 1909) 
                   
               
               
                 GUGAGGCUGGAGUGAGCGGAGAUCGUACCACUGCACUCCAACCUGGUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4431 MI0016771 (SEQ ID NO: 1910) 
                   
               
               
                 UGGUUUGCGACUCUGAAAACUAGAAGGUUUAUGACUGGGCAUUUCUCACCCAAUGCCCAAUAUUGAACUUUCUAGUU 
                   
               
               
                 GUCAGAGUCAUUAACCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4432 MI0016772 (SEQ ID NO: 1911) 
                   
               
               
                 GCAUCUUGCAGAGCCGUUCCAAUGCGACACCUCUAGAGUGUCAUCCCCUAGAAUGUCACCUUGGAAAGACUCUGCAA 
                   
               
               
                 GAUGCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4433 MI0016773 (SEQ ID NO: 1912) 
                   
               
               
                 CAUCCUCCUUACGUCCCACCCCCCACUCCUGUUUCUGGUGAAAUAUUCAAACAGGAGUGGGGGUGGGACAUAAGGAG 
                   
               
               
                 GAUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4434 MI0016774 (SEQ ID NO: 1913) 
                   
               
               
                 UCACUUUAGGAGAAGUAAAGUAGAACUUUGGUUUUCAACUUUUCCUACAGUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4435-1 MI0016775 (SEQ ID NO: 1914) 
                   
               
               
                 AGGCAGCAAAUGGCCAGAGCUCACACAGAGGGAUGAGUGCACUUCACCUGCAGUGUGACUCAGCAGGCCAACAGAUG 
                   
               
               
                 CUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4435-2 MI0016777 (SEQ ID NO: 1915) 
                   
               
               
                 GCAAAUGGCCAGAGCUCACACAGAGGGAUGAGUGCACUUCACCUGCAGUGUGACUCAGCAGGCCAACAGAUGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4436a MI0016776 (SEQ ID NO: 1916) 
                   
               
               
                 GCCUCACUUUUCCACUUAUGCCUGCCCUGCCCCUCGAAUCUGCUCCACGAUUUGGGCAGGACAGGCAGAAGUGGAUA 
                   
               
               
                 AGUGAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4436b-1 MI0017425 (SEQ ID NO: 1917) 
                   
               
               
                 GUGUCCUCACUUGUCCACUUCUGCCUGCCCUGCCCAAAUGGUGGAGCAGAUUCGAGGGGCAGGGCAGGAAGAAGUGG 
                   
               
               
                 ACAAGUGAGGCCAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4436b-2 MI0019110 (SEQ ID NO: 1918) 
                   
               
               
                 GUGUCCUCACUUGUCCACUUCUGCCUGCCCUGCCCAAAUGGUGGAGCAGAUUCGAGGGGCAGGGCAGGAAGAAGUGG 
                   
               
               
                 ACAAGUGAGGCCAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4437 MI0016778 (SEQ ID NO: 1919) 
                   
               
               
                 ACUUUGUGCAUUGGGUCCACAAGGAGGGGAUGACCCUUGUGGGCUCAGGGUACAAAGGUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4438 MI0016781 (SEQ ID NO: 1920) 
                   
               
               
                 UAAGUGUAAACUUAAGGACUGUCUUUUCUAAGCCUGUGCCUUGCCUUUCCUUUGGCACAGGCUUAGAAAAGACAGUC 
                   
               
               
                 UUUAAGUUUACACUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4439 MI0016782 (SEQ ID NO: 1921) 
                   
               
               
                 CCAGUGACUGAUACCUUGGAGGCAUUUUAUCUAAGAUACACACAAAGCAAAUGCCUCUAAGGUAUCAGUUUACCAGG 
                   
               
               
                 CCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4440 MI0016783 (SEQ ID NO: 1922) 
                   
               
               
                 CUCUCACCAAGCAAGUGCAGUGGGGCUUGCUGGCUUGCACCGUGACUCCCUCUCACCAAGCAAGUGUCGUGGGGCUU 
                   
               
               
                 GCUGGCUUGCACUGUGAAGAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4441 MI0016784 (SEQ ID NO: 1923) 
                   
               
               
                 CAGAGUCUCCUUCGUGUACAGGGAGGAGACUGUACGUGAGAGAUAGUCAGAUCCGCAUGUUAGAGCAGAGUCUCCUU 
                   
               
               
                 CGUGUACAGGGAGGAGAUUGUAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4442 MI0016785 (SEQ ID NO: 1924) 
                   
               
               
                 GCGCCCUCCCUCUCUCCCCGGUGUGCAAAUGUGUGUGUGCGGUGUUAUGCCGGACAAGAGGGAGGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4443 MI0016786 (SEQ ID NO: 1925) 
                   
               
               
                 GGUGGGGGUUGGAGGCGUGGGUUUUAGAACCUAUCCCUUUCUAGCCCUGAGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4444-1 MI0016787 (SEQ ID NO: 1926) 
                   
               
               
                 GUGACGACUGGCCCCGCCUCUUCCUCUCGGUCCCAUAUUGAACUCGAGUUGGAAGAGGCGAGUCCGGUCUCAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4444-2 MI0019111 (SEQ ID NO: 1927) 
                   
               
               
                 GUGACGACUGGCCCCGCCUCUUCCUCUCGGUCCCAUAUUGAACUCGAGUUGGAAGAGGCGAGUCCGGUCUCAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4445 MI0016788 (SEQ ID NO: 1928) 
                   
               
               
                 UUCCUGCAGAUUGUUUCUUUUGCCGUGCAAGUUUAAGUUUUUGCACGGCAAAAGAAACAAUCCAGAGGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4446 MI0016789 (SEQ ID NO: 1929) 
                   
               
               
                 CUGGUCCAUUUCCCUGCCAUUCCCUUGGCUUCAAUUUACUCCCAGGGCUGGCAGUGACAUGGGUCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4447 MI0016790 (SEQ ID NO: 1930) 
                   
               
               
                 GUUCUAGAGCAUGGUUUCUCAUCAUUUGCACUACUGAUACUUGGGGUCAGAUAAUUGUUUGUGGUGGGGGCUGUUGU 
                   
               
               
                 UUGCAUUGUAGGAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4448 MI0016791 (SEQ ID NO: 1931) 
                   
               
               
                 AGGAGUGACCAAAAGACAAGAGUGCGAGCCUUCUAUUAUGCCCAGACAGGGCCACCAGAGGGCUCCUUGGUCUAGGG 
                   
               
               
                 GUAAUGCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4449 MI0016792 (SEQ ID NO: 1932) 
                   
               
               
                 AGCAGCCCUCGGCGGCCCGGGGGGCGGGCGGCGGUGCCCGUCCCGGGGCUGCGCGAGGCACAGGCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4450 MI0016795 (SEQ ID NO: 1933) 
                   
               
               
                 UGUCUGGGGAUUUGGAGAAGUGGUGAGCGCAGGUCUUUGGCACCAUCUCCCCUGGUCCCUUGGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4451 MI0016797 (SEQ ID NO: 1934) 
                   
               
               
                 UCUGUACCUCAGCUUUGCUCCCAACCAACCACUUCCACAUGUUUUGCUGGUAGAGCUGAGGACAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4452 MI0016798 (SEQ ID NO: 1935) 
                   
               
               
                 UGGAUCACUUGAGGCCAAGAGUGCAAGGCUGUAGUGUGCACAGCCUUGAAUUCUUGGCCUUAAGUGAUCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4453 MI0016799 (SEQ ID NO: 1936) 
                   
               
               
                 UGGAGAGCUUGGUCUGUAGCGGUUUCCUUCGGGGCAGGUGGGGACUGCUCCUUUGGGAGGAAGGAGGAGGCCCAGGC 
                   
               
               
                 CGCGUCUUCAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4454 MI0016800 (SEQ ID NO: 1937) 
                   
               
               
                 CCGGAUCCGAGUCACGGCACCAAAUUUCAUGCGUGUCCGUGUGAAGAGACCACCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4455 MI0016801 (SEQ ID NO: 1938) 
                   
               
               
                 AGAAGGGUGUGUGUGUUUUUCCUGAGAAUAAGAGAAGGAAGGACAGCCAAAUUCUUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4456 MI0016802 (SEQ ID NO: 1939) 
                   
               
               
                 AUGAACCUGGUGGCUUCCUUUUCUGGGAGGAAGUUAGGGUUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4457 MI0016803 (SEQ ID NO: 1940) 
                   
               
               
                 GGAGUACUCCAGUCAAUACCGUGUGAGUUAGAAAAGCUCAAUUCACAAGGUAUUGACUGGCGUAUUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4458 MI0016804 (SEQ ID NO: 1941) 
                   
               
               
                 GAGCGCACAGAGGUAGGUGUGGAAGAAAGUGAAACACUAUUUUAGGUUUUAGUUACACUCUGCUGUGGUGUGCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4459 MI0016805 (SEQ ID NO: 1942) 
                   
               
               
                 ACCCAGGAGGCGGAGGAGGUGGAGGUUGCAGUGAGCCAAGAUCGUGGCACUGACUCCAGCCUGGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4460 MI0016806 (SEQ ID NO: 1943) 
                   
               
               
                 GUUUUUUGCCCAUAGUGGUUGUGAAUUUACCUUCUCCUCUUUGCAGUGAUAAAGGAGGUAAAUUCACAACCACUGUG 
                   
               
               
                 GGCAGAAAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4461 MI0016807 (SEQ ID NO: 1944) 
                   
               
               
                 GAGUAGGCUUAGGUUAUGUACGUAGUCUAGGCCAUACGUGUUGGAGAUUGAGACUAGUAGGGCUAGGCCUACUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4462 MI0016810 (SEQ ID NO: 1945) 
                   
               
               
                 CUUCCCAGCUGCCCUAAGUCAGGAGUGGCUUUCCUGACACGGAGGGUGGCUUGGGAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4463 MI0016811 (SEQ ID NO: 1946) 
                   
               
               
                 AAUAGAUUAUUGGUCACCACCUCCAGUUUCUGAAUUUGUGAGACUGGGGUGGGGCCUGAGAAUUUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4464 MI0016812 (SEQ ID NO: 1947) 
                   
               
               
                 GGAACCUUAGUAAGGUUUGGAUAGAUGCAAUAAAGUAUGUCCACAGCUGAAAGGACAUACUUUAUUGCAUGUAUCCA 
                   
               
               
                 AACCUUACUAAUUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4465 MI0016816 (SEQ ID NO: 1948) 
                   
               
               
                 CAUGUGUCCCCUGGCACGCUAUUUGAGGUUUACUAUGGAACCUCAAGUAGUCUGACCAGGGGACACAUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4466 MI0016817 (SEQ ID NO: 1949) 
                   
               
               
                 ACGCGGGUGCGGGCCGGCGGGGUAGAAGCCACCCGGCCCGGCCCGGCCCGGCGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4467 MI0016818 (SEQ ID NO: 1950) 
                   
               
               
                 UGGUGGCGGCGGUAGUUAUGGGCUUCUCUUUCUCACCAGCAGCCCCUGGGCCGCCGCCUCCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4468 MI0016819 (SEQ ID NO: 1951) 
                   
               
               
                 AGUCUUCUCCUGGGGCUUUGGUGGCUAUGGUUGACUGGGCCACUCAGAGCAGAAGGAUGAGAUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4469 MI0016820 (SEQ ID NO: 1952) 
                   
               
               
                 CCGACGCGGAGAGCGGCUCUAGGUGGGUUUGGCGGCGGCGAGGACACCGCCGCUCCCUCUAGGGUCGCUCGGAGCGU 
                   
               
               
                 GA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4470 MI0016821 (SEQ ID NO: 1953) 
                   
               
               
                 CGAGCCUCUUUCGGCUUUCCAGUUUGUCUCGGUCCUUUGGAACGUGGCAAACGUGGAAGCCGAGAGGGCUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4471 MI0016822 (SEQ ID NO: 1954) 
                   
               
               
                 CCAAAUUUAAAACUUAAACCUCUACUAAGUUUCCAUGAAAAGAACCCAUGGGAACUUAGUAGAGGUUUAAGUUUUAA 
                   
               
               
                 AUUUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4472-1 MI0016823 (SEQ ID NO: 1955) 
                   
               
               
                 UGGCAGACCCUUGCUCUCUCACUCUCCCUAAUGGGGCUGAAGACAGCUCAGGGGCAGGGUGGGGGGUGUUGUUUUUG 
                   
               
               
                 UUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4472-2 MI0016824 (SEQ ID NO: 1956) 
                   
               
               
                 UGGUGGGGGUGGGGGGUGUUGUUUUUGUUUUUGAGACAGAGUCUUGCUCCGUCGCCCAGGCCGGAGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4473 MI0016825 (SEQ ID NO: 1957) 
                   
               
               
                 AAGGAACAGGGGACACUUGUAAUGGAGAACACUAAGCUAUGGACUGCUAUGGACUGCUAGUGCUCUCCGUUACAAGU 
                   
               
               
                 AUCCCCUGUUACCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4474 MI0016826 (SEQ ID NO: 1958) 
                   
               
               
                 UUGCCUACCUUGUUAGUCUCAUGAUCAGACACAAAUAUGGCUCUUUGUGGCUGGUCAUGAGGCUAACAAGGUAGGCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4475 MI0016827 (SEQ ID NO: 1959) 
                   
               
               
                 AUCUCAAUGAGUGUGUGGUUCUAAAUGACUCAUAGUCAAGGGACCAAGCAUUCAUUAUGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4476 MI0016828 (SEQ ID NO: 1960) 
                   
               
               
                 AAAAGCCUGUCCCUAAGUCCCUCCCAGCCUUCCAGAGUUGGUGCCAGGAAGGAUUUAGGGACAGGCUUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4477a MI0016829 (SEQ ID NO: 1961) 
                   
               
               
                 UCCUCCUCCCAUCAAUCACAAAUGUCCUUAAUGGCAUUUAAGGAUUGCUAUUAAGGACAUUUGUGAUUCACGGGAGG 
                   
               
               
                 AGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4477b MI0016830 (SEQ ID NO: 1962) 
                   
               
               
                 ACCUCCUCCCGUGAAUCACAAAUGUCCUUAAUAGCAAUCCUUAAAUGCCAUUAAGGACAUUUGUGAUUGAUGGGAGG 
                   
               
               
                 AGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4478 MI0016831 (SEQ ID NO: 1963) 
                   
               
               
                 GGCCGAGGCUGAGCUGAGGAGCCUCCAAACCUGUAGACAGGGUCAUGCAGUACUAGGGGCGAGCCUCAUCCCCUGCA 
                   
               
               
                 GCCCUGGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4479 MI0016838 (SEQ ID NO: 1964) 
                   
               
               
                 GAAACCAAGUCCGAGCGUGGCUGGCGCGGGAAAGUUCGGGAACGCGCGCGGCCGUGCUCGGAGCAGCGCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4480 MI0016841 (SEQ ID NO: 1965) 
                   
               
               
                 GCAGAGGUGAGUUGACCUCCACAGGGCCACCCAGGGAGUAAGUAGCCAAGUGGAAGUUACUUUACCUCUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4481 MI0016842 (SEQ ID NO: 1966) 
                   
               
               
                 GGAGUGGGCUGGUGGUUUUUUAAGAGGAAGGGAGACCUAAGCUAGCACAUGAGCACGCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4482-1 MI0016843 (SEQ ID NO: 1967) 
                   
               
               
                 AGUGAGCAACCCAGUGGGCUAUGGAAAUGUGUGGAAGAUGGCAUUUCUAUUUCUCAGUGGGGCUCUUACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4482-2 MI0017872 (SEQ ID NO: 1968) 
                   
               
               
                 AAUAAAAAUUGAAUAGUGAGCAACCCAGUGGGCUAUGGAAAUGUGUGGAAGAUGGCAUUUCUAUUUCUCAGUGGGGC 
                   
               
               
                 UCUUACCUAUUACUCAUCAAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4483 MI0016844 (SEQ ID NO: 1969) 
                   
               
               
                 AAAAAACAACAUACUUAGUGCAUACCCAUAUAAUAUUAGGGGUGGUCUGUUGUUGUUUUUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4484 MI0016845 (SEQ ID NO: 1970) 
                   
               
               
                 GGGUUUCCUCUGCCUUUUUUUCCAAUGAAAAUAACGAAACCUGUUAUUUCCCAUUGAGGGGGAAAAAGGCGGGAGAA 
                   
               
               
                 GCCCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4485 MI0016846 (SEQ ID NO: 1971) 
                   
               
               
                 AGAGGCACCGCCUGCCCAGUGACAUGCGUUUAACGGCCGCGGUACCCUAACUGUGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4486 MI0016847 (SEQ ID NO: 1972) 
                   
               
               
                 GCAUGCUGGGCGAGGCUGGCAUCUAGCACAGGCGGUAGAUGCUUGCUCUUGCCAUUGCAAUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4487 MI0016848 (SEQ ID NO: 1973) 
                   
               
               
                 ACUGUCCUUCAGCCAGAGCUGGCUGAAGGGCAGAAGGGAACUGUCCUUCAGCCAGAGCUGGCUGAAGGGCAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4488 MI0016849 (SEQ ID NO: 1974) 
                   
               
               
                 GGUAGGGGGCGGGCUCCGGCGCUGGGACCCCACUAGGGUGGCGCCUUGGCCCCGCCCCGCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4489 MI0016850 (SEQ ID NO: 1975) 
                   
               
               
                 GGGGGUGGGGCUAGUGAUGCAGGACGCUGGGGACUGGAGAAGUCCUGCCUGACCCUGUCCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4490 MI0016852 (SEQ ID NO: 1976) 
                   
               
               
                 AUAGUUUCUGCAAUGCUCAAAUCUCUGGCCAAAGACCAGAACUUAAUGGUCUCUGGUAAGAGAUUUGGGCAUAUUAG 
                   
               
               
                 AAACUAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4491 MI0016853 (SEQ ID NO: 1977) 
                   
               
               
                 ACAUUUGGUCACACCAGUCCACAUUAACGUGGACCAGACAAUAUUAAUGUGGACUGGUGUGACCAAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4492 MI0016854 (SEQ ID NO: 1978) 
                   
               
               
                 CUGCAGCGUGCUUCUCCAGGCCCCGCGCGCGGACAGACACACGGACAAGUCCCGCCAGGGGCUGGGCGCGCGCCAGC 
                   
               
               
                 CGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4493 MI0016855 (SEQ ID NO: 1979) 
                   
               
               
                 CCAGAGAUGGGAAGGCCUUCCGGUGAUUAUCACAGCCAUGCCUUUACCUCCAGAAGGCCUUUCCAUCUCUGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4494 MI0016856 (SEQ ID NO: 1980) 
                   
               
               
                 AGUUUUAGUUACCCUGGUCAUCUGCAGUCUGAAAAUACAAAAUGGAAAAUUCCAGACUGUGGCUGACCAGAGGUAAC 
                   
               
               
                 UGAAACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4495 MI0016857 (SEQ ID NO: 1981) 
                   
               
               
                 AAGAAAUGUAAACAGGCUUUUUGCUCAGUGGAGUUAUUUUGAGCAAAAAGCUUAUUUACAUUUCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4496 MI0016858 (SEQ ID NO: 1982) 
                   
               
               
                 ACAUCAGCUCAUAUAAUCCUCGAAGCUGCCUUUAGAAAUGAGGAAACUGAAGCUGAGAGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4497 MI0016859 (SEQ ID NO: 1983) 
                   
               
               
                 ACCUCCGGGACGGCUGGGCGCCGGCGGCCGGGAGAUCCGCGCUUCCUGAAUCCCGGCCGGCCCGCCCGGCGCCCGUC 
                   
               
               
                 CGCCCGCGGGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4498 MI0016860 (SEQ ID NO: 1984) 
                   
               
               
                 AGGGCUGGGCUGGCAGGGCAAGUGCUGCAGAUCUUUGUCUAAGCAGCCCCUGCCUUGGAUCUCCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4499 MI0016862 (SEQ ID NO: 1985) 
                   
               
               
                 AAGACUGAGAGGAGGGAACUGGUGAGUUGUACAUAGAAAUGCUUUCUAACUCCUUGUCUCAGUCUGUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4500 MI0016863 (SEQ ID NO: 1986) 
                   
               
               
                 CAGGAGAGAAAGUACUGCCCAGAAGCUAAAGUGUAGAUCAAACGCAUAAUGGCUGAGGUAGUAGUUUCUUGAACUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4501 MI0016864 (SEQ ID NO: 1987) 
                   
               
               
                 UAUGUGACCUCGGAUGAAUCACUGAAAUAUGUCUGAGCUUCUGUUUCAUCAGAUGUCACAUUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4502 MI0016865 (SEQ ID NO: 1988) 
                   
               
               
                 AGCCUUUAGCAAGUUGUAAUCUUUUUGCUGAUGGAGGGUCUUGCCUCCAUGGGGAUGGCUGAUGAUGAUGGUGCUGA 
                   
               
               
                 AGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4503 MI0016866 (SEQ ID NO: 1989) 
                   
               
               
                 ACAAUGUAGAUAUUUAAGCAGGAAAUAGAAUUUACAUAUAAAUUUCUAUUUGUUUCUAUUUCCUGCUUAAAUAUCUA 
                   
               
               
                 CAUUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4504 MI0016867 (SEQ ID NO: 1990) 
                   
               
               
                 CUAAGAUAAUGUCCUCCAGGUUCAUCUCUGUUGUCAUUUGUGGCAUGGACCAUUUGUGACAAUAGAGAUGAACAUGG 
                   
               
               
                 AGGAUAUUAUCUUAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4505 MI0016868 (SEQ ID NO: 1991) 
                   
               
               
                 GGAGGCUGGGCUGGGACGGACACCCGGCCUCCACUUUCUGUGGCAGGUACCUCCUCCAUGUCGGCCCGCCUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4506 MI0016869 (SEQ ID NO: 1992) 
                   
               
               
                 UGGCCUCUGCCAUCAGACCAUCUGGGUUCAAGUUUGGCUCCAUCUUUAUGAAAUGGGUGGUCUGAGGCAAGUGGUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4507 MI0016871 (SEQ ID NO: 1993) 
                   
               
               
                 UCUGGGCUGAGCCGAGCUGGGUUAAGCCGAGCUGGGUUGGGCUGGGCUGGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4508 MI0016872 (SEQ ID NO: 1994) 
                   
               
               
                 AGGACCCAGCGGGGCUGGGCGCGCGGAGCAGCGCUGGGUGCAGCGCCUGCGCCGGCAGCUGCAAGGGCCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4509-1 MI0016873 (SEQ ID NO: 1995) 
                   
               
               
                 CUUUAAUACUAUCUCAAACUAAAGGAUAUAGAAGGUUUUCCCUUUCUCUUGCCCUGAAACCUUCUGUAUCCUUUAUU 
                   
               
               
                 UUGAGAUAGUAUUAGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4509-2 MI0016874 (SEQ ID NO: 1996) 
                   
               
               
                 CUUUAAUACUAUCUCAAACUAAAGGAUAUAGAAGGUUUUCCCUUUCUCUUGCCCUGAAACCUUCUGUAUCCUUUAUU 
                   
               
               
                 UUGAGAUAGUAUUAGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4509-3 MI0016875 (SEQ ID NO: 1997) 
                   
               
               
                 CUUUAAUACUAUCUCAAACUAAAGGAUAUAGAAGGUUUUCCCUUUCUCUUGCCCUGAAACCUUCUGUAUCCUUUAUU 
                   
               
               
                 UUGAGAUAGUAUUAGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4510 MI0016876 (SEQ ID NO: 1998) 
                   
               
               
                 GUGUAUGUGAGGGAGUAGGAUGUAUGGUUGUUAGAUAGACAACUACAAUCUUUUCUCACAACAGACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4511 MI0016877 (SEQ ID NO: 1999) 
                   
               
               
                 AAAAAAAAGGGAAAGAAGAACUGUUGCAUUUGCCCUGCACUCAGUUUGCACAGGGUAAAUGCAAUAGUUCUUCUUUC 
                   
               
               
                 CCUUUUUUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4512 MI0016878 (SEQ ID NO: 2000) 
                   
               
               
                 CUCAGCCCGGGCAAUAUAGUGAGACCUCGUCUCUACAAAAAAUUGAGACAGGGCCUCACUGUAUCGCCCAGGCUGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4513 MI0016879 (SEQ ID NO: 2001) 
                   
               
               
                 AUUCUAGGUGGGGAGACUGACGGCUGGAGGCCCAUAAGCUGUCUAAAACUUCGGCCCCCAGAUUUCUGGUCUCCCCA 
                   
               
               
                 CUUCAGAAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4514 MI0016880 (SEQ ID NO: 2002) 
                   
               
               
                 GUUGAGACAGGCAGGAUUGGGGAAACAUCUUUUACCUCGUCUCUUGCCUGUUUUAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4515 MI0016881 (SEQ ID NO: 2003) 
                   
               
               
                 GCGGGAGGUGUAACAGGACUGGACUCCCGGCAGCCCCAGGGCAGGGGCGUGGGGAGCUGGUCCUAGCUCAGCGCUCC 
                   
               
               
                 CGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4516 MI0016882 (SEQ ID NO: 2004) 
                   
               
               
                 AGGGAGAAGGGUCGGGGCAGGGAGGGCAGGGCAGGCUCUGGGGUGGGGGGUCUGUGAGUCAGCCACGGCUCUGCCCA 
                   
               
               
                 CGUCUCCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4517 MI0016883 (SEQ ID NO: 2005) 
                   
               
               
                 AGGUAAAUAUGAUGAAACUCACAGCUGAGGAGCUUAGCAAGUAGCUAAGGCCAGAGCUUGUGUUUGGGUGGUGUGGC 
                   
               
               
                 UG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4518 MI0016884 (SEQ ID NO: 2006) 
                   
               
               
                 UGGGGGAAAAGUGCUGGGAUUGAUUAGUGAUGUCUGCUGGGGAACCGGGGCUCAGGGAUGAUAACUGUGCUGAGAAG 
                   
               
               
                 CCCCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4519 MI0016885 (SEQ ID NO: 2007) 
                   
               
               
                 AACCUCAGCAGUGCGCAGGGCUGCACUGUCUCCGUCUGCGGCCUGCAGUAAGCGGGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4520a MI0016886 (SEQ ID NO: 2008) 
                   
               
               
                 GUGUGCCACCUGCGUGUUUUCUGUCCAAAUCAGAAAAGGAUUUGGACAGAAAACACGCAGGAAGAAGGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4520b MI0017358 (SEQ ID NO: 2009) 
                   
               
               
                 CCUGCGUGUUUUCUGUCCAAAUCCUUUUCUGAUUUGGACAGAAAACACGCAGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4521 MI0016887 (SEQ ID NO: 2010) 
                   
               
               
                 UCGGCUAAGGAAGUCCUGUGCUCAGUUUUGUAGCAUCAAAACUAGGAUUUCUCUUGUUAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4522 MI0016889 (SEQ ID NO: 2011) 
                   
               
               
                 GCGGGCGUUGCCUGGGGGCCUCGCAGGGGGAGAUCCAGCCCAGGCUGGUUCCGCUGACUCUGCCUGUAGGCCGGUGG 
                   
               
               
                 CGUCUUCUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4523 MI0016890 (SEQ ID NO: 2012) 
                   
               
               
                 GCGGGGGACCGAGAGGGCCUCGGCUGUGUGAGGACUAGAGGCGGCCGAGGCCCGGGCCGGUUCCCCCGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4524a MI0016891 (SEQ ID NO: 2013) 
                   
               
               
                 GAACGAUAGCAGCAUGAACCUGUCUCACUGCAGAAUUAUUUUGAGACAGGCUUAUGCUGCUAUCCUUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4524b MI0019114 (SEQ ID NO: 2014) 
                   
               
               
                 UAGCUGGGUGGAUGUGUUCUUUUGAAGGAUAGCAGCAUAAGCCUGUCUCAAAAUAAUUCUGCAGUGAGACAGGUUCA 
                   
               
               
                 UGCUGCUAUCGUUCCAAAGAGGAAGGGUAAUCACUGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4525 MI0016892 (SEQ ID NO: 2015) 
                   
               
               
                 GUCAGAGGGGGGAUGUGCAUGCUGGUUGGGGUGGGCUGCCUGUGGACCAAUCAGCGUGCACUUCCCCACCCUGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4526 MI0016893 (SEQ ID NO: 2016) 
                   
               
               
                 UGCGGUGACAUCAGGGCCCAGUCCCUGCUGUCAUGCCCCAGGUGACGUGCUGGGCUGACAGCAGGGCUGGCCGCUAA 
                   
               
               
                 CGUCACUGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4527 MI0016894 (SEQ ID NO: 2017) 
                   
               
               
                 CCAGAAGUGGUCUGCAAAGAGAUGACUGUGAAUCCAAGAUCCACAUCAGCUCUGUGCUGCCUACAUCUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4528 MI0016895 (SEQ ID NO: 2018) 
                   
               
               
                 UAUUCUACUGAGAGUACAGAUCUUUAUAUAUAUGAUCAUUAUAUGUAUGAUGAGAUCAUUAUAUGUAUGAUCUGGAC 
                   
               
               
                 ACCCAGUAGAAUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4529 MI0016896 (SEQ ID NO: 2019) 
                   
               
               
                 AUGACAGGCCAUCAGCAGUCCAAUGAAGACAUGAAGACCCAAUGUCUUCAUUGGACUGCUGAUGGCCCGUCACUGGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4530 MI0016897 (SEQ ID NO: 2020) 
                   
               
               
                 CGACCGCACCCGCCCGAAGCUGGGUCAAGGAGCCCAGCAGGACGGGAGCGCGGCGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4531 MI0016898 (SEQ ID NO: 2021) 
                   
               
               
                 GCCUAGGAGUCCUUGGUCAGUGGGGACAUGGAGAAGGCUUCUGAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4532 MI0016899 (SEQ ID NO: 2022) 
                   
               
               
                 ACAGACCCCGGGGAGCCCGGCGGUGAAGCUCCUGGUAUCCUGGGUGUCUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4533 MI0016900 (SEQ ID NO: 2023) 
                   
               
               
                 UGAGAAUGUGGAAGGAGGUUGCCGGACGCUGCUGGCUGCCUUCCAGCGUCCACUUCCCUUUCUCUCUCUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4534 MI0016901 (SEQ ID NO: 2024) 
                   
               
               
                 UGUGAAUGACCCCCUUCCAGAGCCAAAAUCACCAGGGAUGGAGGAGGGGUCUUGGGUACU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4535 MI0016903 (SEQ ID NO: 2025) 
                   
               
               
                 AACUGGGUCCCAGUCUUCACAGUUGGUUUCUGACACGUGGACCUGGCUGGGACGAUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4536-1 MI0016906 (SEQ ID NO: 2026) 
                   
               
               
                 AUGUGGUAGAUAUAUGCACGAUAUAUAUACUGCCCUGCUUUUAUACAUACAUACAUACAUACCUAUAUCGUGCAUAU 
                   
               
               
                 AUCUACCACAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4536-2 MI0019149 (SEQ ID NO: 2027) 
                   
               
               
                 AUGUGGUAGAUAUAUGCACGAUAUAGGUAUGUAUGUAUGUAUGUAUAAAAGCAGGGCAGUAUAUAUAUCGUGCAUAU 
                   
               
               
                 AUCUACCACAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4537 MI0016908 (SEQ ID NO: 2028) 
                   
               
               
                 UGAGCCGAGCUGAGCUUAGCUGGGCUGAGCUAACCAGGGCUGGGCUGAGCUGGGCUGAGCUGAGCUGAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4538 MI0016909 (SEQ ID NO: 2029) 
                   
               
               
                 GAGCUUGGAUGAGCUGGGCUGAACUGGGCUGGGUUGAGCUGGGCUGGGCUGAGUUGAGCCAGGCUGAUCUGGGCUGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4539 MI0016910 (SEQ ID NO: 2030) 
                   
               
               
                 UGAGCUGGGCUCUGCUGUGCUGUGCUGAGCAGGGCUGAGCUGAACUGGGCUGAGCUGGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4540 MI0016911 (SEQ ID NO: 2031) 
                   
               
               
                 AAGCUGCAUGGACCAGGACUUGGCACCUUUGGCCUUAGUCCUGCCUGUAGGUUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4632 MI0017259 (SEQ ID NO: 2032) 
                   
               
               
                 GAGGGCAGCGUGGGUGUGGCGGAGGCAGGCGUGACCGUUUGCCGCCCUCUCGCUGCUCUAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4633 MI0017260 (SEQ ID NO: 2033) 
                   
               
               
                 UGGCAAGUCUCCGCAUAUGCCUGGCUAGCUCCUCCACAAAUGCGUGUGGAGGAGCUAGCCAGGCAUAUGCAGAGCGU 
                   
               
               
                 CA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4634 MI0017261 (SEQ ID NO: 2034) 
                   
               
               
                 GGACAAGGGCGGCGCGACCGGCCCGGGGCUCUUGGGCGGCCGCGUUUCCCCUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4635 MI0017262 (SEQ ID NO: 2035) 
                   
               
               
                 CCGGGACUUUGUGGGUUCUGACCCCACUUGGAUCACGCCGACAACACUGGUCUUGAAGUCAGAACCCGCAAAGUCCU 
                   
               
               
                 GG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4636 MI0017263 (SEQ ID NO: 2036) 
                   
               
               
                 UAGAUUCAGAACUCGUGUUCAAAGCCUUUAGCCCAGCAAUGGGAGAGUGCUAAAGGCUUCAAGCACGAGUUCUGAAU 
                   
               
               
                 CUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4637 MI0017264 (SEQ ID NO: 2037) 
                   
               
               
                 CCCUUACUUGGAUCUGCAAUUAGUAUUUUAAUCAUAGAUUGUAUUUAGUUAGUUUUUAAUACUAACUGCAGAUUCAA 
                   
               
               
                 GUGAGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4638 MI0017265 (SEQ ID NO: 2038) 
                   
               
               
                 GACUCGGCUGCGGUGGACAAGUCCGGCUCCAGAACCUGGACACCGCUCAGCCGGCCGCGGCAGGGGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4639 MI0017266 (SEQ ID NO: 2039) 
                   
               
               
                 UUGCUAAGUAGGCUGAGAUUGAUGUCAGGUUAUCCCCAAGCAUAACCUCACUCUCACCUUGCUUUGCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4640 MI0017267 (SEQ ID NO: 2040) 
                   
               
               
                 CUGUGGGCUGGGCCAGGGAGCAGCUGGUGGGUGGGAAGUAAGAUCUGACCUGGACUCCAUCCCACCCACCCCCUGUU 
                   
               
               
                 UCCUGGCCCACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4641 MI0017268 (SEQ ID NO: 2041) 
                   
               
               
                 GGGGGGCAGGGGGCAGAGGGCAUCAGAGGACAGCCGCCUGGUGCCCAUGCCAUACUUUUGCCUCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4642 MI0017269 (SEQ ID NO: 2042) 
                   
               
               
                 CACAACUGCAUGGCAUCGUCCCCUGGUGGCUGUGGCCUAGGGCAAGCCACAAAGCCACUCAGUGAUGAUGCCAGCAG 
                   
               
               
                 UUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4643 MI0017270 (SEQ ID NO: 2043) 
                   
               
               
                 GUGUGCCCUAGCAUUUAUAAUCAUGUGUUCAUUCACAUGAUCAUAAGUGGACACAUGACCAUAAAUGCUAAAGCACAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4644 MI0017271 (SEQ ID NO: 2044) 
                   
               
               
                 GCGGCGGUGCUCUGCCUCUUUCUCCAUCCACCCUGGUCCAGGUCCACAGCAGUGGAGAGAGAAAAGAGACAGAAGGA 
                   
               
               
                 UGGCCGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4645 MI0017272 (SEQ ID NO: 2045) 
                   
               
               
                 UGAUAGGGAAACCAGGCAAGAAAUAUUGUCUCCUCAAGUUGCGACGAGACAGUAGUUCUUGCCUGGUUUCUCUAUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4646 MI0017273 (SEQ ID NO: 2046) 
                   
               
               
                 ACUGGGAAGAGGAGCUGAGGGACAUUGCGGAGAGGGUCUCACAUUGUCCCUCUCCCUUCCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4647 MI0017274 (SEQ ID NO: 2047) 
                   
               
               
                 CCAGGAGGGUGAAGAUGGUGCUGUGCUGAGGAAAGGGGAUGCAGAGCCCUGCCCAGCACCACCACCUCCUAUGCUCC 
                   
               
               
                 UGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4648 MI0017275 (SEQ ID NO: 2048) 
                   
               
               
                 UGUGGGACUGCAAAUGGGAGCUCAGCACCUGCCUGCCACCCACGCAGACCAGCCCCUGCUCUGUUCCCACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4649 MI0017276 (SEQ ID NO: 2049) 
                   
               
               
                 UCUGGGCGAGGGGUGGGCUCUCAGAGGGGCUGGCAGUACUGCUCUGAGGCCUGCCUCUCCCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4650-1 MI0017277 (SEQ ID NO: 2050) 
                   
               
               
                 UUCUGUAGAGAUUAUCAGGCCUCUUUCUACCUUCCAAGGCUCAGAAGGUAGAAUGAGGCCUGACAUAUCUGCAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4650-2 MI0017278 (SEQ ID NO: 2051) 
                   
               
               
                 UUCUGUAGAGAUUAUCAGGCCUCUUUCUACCUUCCAAGGCUCAGAAGGUAGAAUGAGGCCUGACAUAUCUGCAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4651 MI0017279 (SEQ ID NO: 2052) 
                   
               
               
                 CGGCGACGGCGGGGUGGGUGAGGUCGGGCCCCAAGACUCGGGGUUUGCCGGGCGCCUCAGUUCACCGCGGCCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4652 MI0017280 (SEQ ID NO: 2053) 
                   
               
               
                 UAUUGGACGAGGGGACUGGUUAAUAGAACUAACUAACCAGAACUAUUUUGUUCUGUUAACCCAUCCCCUCAUCUAAUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4653 MI0017281 (SEQ ID NO: 2054) 
                   
               
               
                 UUGUCCAAUUCUCUGAGCAAGGCUUAACACCAAAGGGUUAAGGGUUUGCUCUGGAGUUAAGGGUUGCUUGGAGAAUU 
                   
               
               
                 GGAGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4654 MI0017282 (SEQ ID NO: 2055) 
                   
               
               
                 CUGGCUGGUUGUGGGAUCUGGAGGCAUCUGGGGUUGGAAUGUGACCCCAGUCUCCUUUUCCCUCAUCAUCUGCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4655 MI0017283 (SEQ ID NO: 2056) 
                   
               
               
                 CCAAGGGCACACCGGGGAUGGCAGAGGGUCGUGGGAAAGUGUUGACCCUCGUCAGGUCCCCGGGGAGCCCCUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4656 MI0017284 (SEQ ID NO: 2057) 
                   
               
               
                 AGGCUGGCGUGGGCUGAGGGCAGGAGGCCUGUGGCCGGUCCCAGGCCUCCUGCUUCCUGGGCUCAGGCUCGGUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4657 MI0017285 (SEQ ID NO: 2058) 
                   
               
               
                 AAUGUGGAAGUGGUCUGAGGCAUAUAGAGUAUAUGCCAAGAACACUACCAUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4658 MI0017286 (SEQ ID NO: 2059) 
                   
               
               
                 GCUGCCCUUCACUCAGAGCAUCUACACCCACUACCGGUGAGUGUGGAUCCUGGAGGAAUCGUGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4659a MI0017287 (SEQ ID NO: 2060) 
                   
               
               
                 GAAACUGCUGAAGCUGCCAUGUCUAAGAAGAAAACUUUGGAGAAAAAUUUUCUUCUUAGACAUGGCAACGUCAACAG 
                   
               
               
                 UUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4659b MI0017291 (SEQ ID NO: 2061) 
                   
               
               
                 CUGUUGACGUUGCCAUGUCUAAGAAGAAAAUUUUUCUCCAAAGUUUUCUUCUUAGACAUGGCAGCUUCAGCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4660 MI0017288 (SEQ ID NO: 2062) 
                   
               
               
                 ACUCCUUCUGCAGCUCUGGUGGAAAAUGGAGAAGACUUUUCCUUUCCUCCAUCUCCCCCAGGGCCUGGUGGAGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4661 MI0017289 (SEQ ID NO: 2063) 
                   
               
               
                 UUUACUCUGAACUAGCUCUGUGGAUCCUGACAGACAGCCUGAUAGACAGGAUCCACAGAGCUAGUCCAGAGUAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4662a MI0017290 (SEQ ID NO: 2064) 
                   
               
               
                 UCUAUUUAGCCAAUUGUCCAUCUUUAGCUAUUCUGAAUGCCUAAAGAUAGACAAUUGGCUAAAUAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4662b MI0017293 (SEQ ID NO: 2065) 
                   
               
               
                 CACAAUUUCUAUUUAGCCAAUUGUCUAUCUUUAGGCAUUCAGAAUAGCUAAAGAUGGACAAUUGGCUAAAUAGACAC 
                   
               
               
                 UGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4663 MI0017292 (SEQ ID NO: 2066) 
                   
               
               
                 CUGUGGUGGAGCUGAGCUCCAUGGACGUGCAGUGGCAUCUGUCAUUGCUGCCUUCCUGGAGCUCAGGCCCUUGCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4664 MI0017294 (SEQ ID NO: 2067) 
                   
               
               
                 GUUGGGGGCUGGGGUGCCCACUCCGCAAGUUAUCACUGAGCGACUUCCGGUCUGUGAGCCCCGUCCUCCGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4665 MI0017295 (SEQ ID NO: 2068) 
                   
               
               
                 CUCGAGGUGCUGGGGGACGCGUGAGCGCGAGCCGCUUCCUCACGGCUCGGCCGCGGCGCGUAGCCCCCGCCACAUCG 
                   
               
               
                 GG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4666a MI0017296 (SEQ ID NO: 2069) 
                   
               
               
                 AUCACUUAAAUACAUGUCAGAUUGUAUGCCUACAAAAUCCCUCCAGACUGGCAUACAAUCUGACAUGUAUUUAAGAG 
                   
               
               
                 AU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4666b MI0019299 (SEQ ID NO: 2070) 
                   
               
               
                 UGUCUAAAUUGCAUGUCAGAUUGUAAUUCCCAGGCCCUUCCUCCAAUACUGGGAAUUACAAUUUGACAUGCAAUUUA 
                   
               
               
                 GACA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4667 MI0017297 (SEQ ID NO: 2071) 
                   
               
               
                 UGACUGGGGAGCAGAAGGAGAACCCAAGAAAAGCUGACUUGGAGGUCCCUCCUUCUGUCCCCACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4668 MI0017298 (SEQ ID NO: 2072) 
                   
               
               
                 AGGGAAAAAAAAAAGGAUUUGUCUUGUAGCCAGGAUAUUGUUUUAAAGAAAAUCCUUUUUGUUUUUCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4669 MI0017300 (SEQ ID NO: 2073) 
                   
               
               
                 GCCUCCCUUCACUUCCUGGCCAUCCAGGCAUCUGUGUCUGUGUCCGGGAAGUGGAGGAGGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4670 MI0017301 (SEQ ID NO: 2074) 
                   
               
               
                 CUCUAGGAAGCGACCAUGAUGUAACUUCACAGACUCUCCAAAAGUCUGAAGUUACAUCAUGGUCGCUUCCUAGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4671 MI0017302 (SEQ ID NO: 2075) 
                   
               
               
                 UAUUUUAAGACCGAAGACUGUGCGCUAAUCUCUUAGCACUGAAGAUUAGUGCAUAGUCUUUGGUCUCAAAAUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4672 MI0017303 (SEQ ID NO: 2076) 
                   
               
               
                 GGCUGCUUCUCGCCUCUGUCCAGCUGUGUGGCCUUGGACAAGCCUCUUGGUUACACAGCUGGACAGAGGCACGAAAC 
                   
               
               
                 AGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4673 MI0017304 (SEQ ID NO: 2077) 
                   
               
               
                 GUCCAGGCAGGAGCCGGACUGGACCUCAGGGAAGAGGCUGACCCGGCCCCUCUUGCGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4674 MI0017305 (SEQ ID NO: 2078) 
                   
               
               
                 CCCAGGCGCCCGCUCCCGACCCACGCCGCGCCGCCGGGUCCCUCCUCCCCGGAGAGGCUGGGCUCGGGACGCGCGGC 
                   
               
               
                 UCAGCUCGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4675 MI0017306 (SEQ ID NO: 2079) 
                   
               
               
                 CAUGAGAAAUCCUGCUGGUCAACCAUAGCCCUGGUCAGACUCUCCGGGGCUGUGAUUGACCAGCAGGACUUCUCAUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4676 MI0017307 (SEQ ID NO: 2080) 
                   
               
               
                 UGAAUGAAAGAGCCAGUGGUGAGACAGUGAGUUGAUUACUUCUCACUGUUUCACCACUGGCUCUUUGGUUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4677 MI0017308 (SEQ ID NO: 2081) 
                   
               
               
                 GCAAAGCAGCAAUUGUUCUUUGGUCUUUCAGCCAUGACCUGACCUUCUGUCUGUGAGACCAAAGAACUACUUUGCUU 
                   
               
               
                 GGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4678 MI0017309 (SEQ ID NO: 2082) 
                   
               
               
                 GGAAAAAACAAGGUAUUGUUCAGACUUAUGAUUUUUGGGGUCAAAGAUUCUGAGCAAUAACCUAUUAAAAAACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4679-1 MI0017310 (SEQ ID NO: 2083) 
                   
               
               
                 GUCUUUUUUCUGUGAUAGAGAUUCUUUGCUUUGUUAGAAACAAAAAGCAAAGAAUCUCUAUCACAGAAAAAAGAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4679-2 MI0017311 (SEQ ID NO: 2084) 
                   
               
               
                 UAUCUUUUUUCUGUGAUAGAGAUUCUUUGCUUUUUGUUUCUAACAAAGCAAAGAAUCUCUAUCACAGAAAAAAGACG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4680 MI0017312 (SEQ ID NO: 2085) 
                   
               
               
                 UAUAAGAACUCUUGCAGUCUUAGAUGUUAUAAAAAUAUAUAUCUGAAUUGUAAGAGUUGUUAGCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4681 MI0017313 (SEQ ID NO: 2086) 
                   
               
               
                 GGCAACGGGAAUGCAGGCUGUAUCUGCAGGGCAUUGUGCUAACAGGUGCAGGCUGCAGACCUGUCACAGGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4682 MI0017314 (SEQ ID NO: 2087) 
                   
               
               
                 UGCCCCUGGUCUGAGUUCCUGGAGCCUGGUCUGUCACUGGGGAAGUCCAGAGCUCCAAGGCUCAGUGCCCAGGGGAC 
                   
               
               
                 GCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4683 MI0017315 (SEQ ID NO: 2088) 
                   
               
               
                 GACACGCAAGACGAGGCGGGCCUGGAGGUGCACCAGUUCUGGCCGCUGGUGGAGAUCCAGUGCUCGCCCGAUCUCAA 
                   
               
               
                 GUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4684 MI0017316 (SEQ ID NO: 2089) 
                   
               
               
                 GCACCAGGGGUACCUCUCUACUGACUUGCAACAUACAUUUGUCUUGGUGUGUUGCAAGUCGGUGGAGACGUACCCUU 
                   
               
               
                 GGUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4685 MI0017317 (SEQ ID NO: 2090) 
                   
               
               
                 UAGCCCAGGGCUUGGAGUGGGGCAAGGUUGUUGGUGAUAUGGCUUCCUCUCCCUUCCUGCCCUGGCUAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4686 MI0017318 (SEQ ID NO: 2091) 
                   
               
               
                 GGCUUCCUGUAUCUGCUGGGCUUUCUGGUGUUGGCAGCCCAAGAUGACACCCUGGGCCCAGCAGGAGCCAGAAGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4687 MI0017319 (SEQ ID NO: 2092) 
                   
               
               
                 ACCUGAGGAGCCAGCCCUCCUCCCGCACCCAAACUUGGAGCACUUGACCUUUGGCUGUUGGAGGGGGCAGGCUCGCG 
                   
               
               
                 GGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4688 MI0017321 (SEQ ID NO: 2093) 
                   
               
               
                 GUCUACUCCCAGGGUGCCAAGCUGUUUCGUGUUCCCUCCCUAGGGGAUCCCAGGUAGGGGCAGCAGAGGACCUGGGC 
                   
               
               
                 CUGGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4689 MI0017322 (SEQ ID NO: 2094) 
                   
               
               
                 GGUUUCUCCUUGAGGAGACAUGGUGGGGGCCGGUCAGGCAGCCCAUGCCAUGUGUCCUCAUGGAGAGGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4690 MI0017323 (SEQ ID NO: 2095) 
                   
               
               
                 GAGCAGGCGAGGCUGGGCUGAACCCGUGGGUGAGGAGUGCAGCCCAGCUGAGGCCUCUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4691 MI0017324 (SEQ ID NO: 2096) 
                   
               
               
                 GGAGCACUCCCAGGUCCUCCAGGCCAUGAGCUGCGGCCCUGAUGUCUCUACUCCAGCCACGGACUGAGAGUGCAUAG 
                   
               
               
                 GAGUGUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4692 MI0017325 (SEQ ID NO: 2097) 
                   
               
               
                 GUUUUACUUGAUACCCACACUGCCUGGGUGGGACACUCAGGCAGUGUGGGUAUCAGAUAAAAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4693 MI0017326 (SEQ ID NO: 2098) 
                   
               
               
                 GUUUAAAGAAUACUGUGAAUUUCACUGUCACAAAUUCAAAUAAAGUGAGAGUGGAAUUCACAGUAUUUAAGGAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4694 MI0017327 (SEQ ID NO: 2099) 
                   
               
               
                 CAAAUACAUAGGUGUUAUCCUAUCCAUUUGCCUCUCUCAGAAAAUAGAGUCAAAUGGACAGGAUAACACCUAUGUAU 
                   
               
               
                 UUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4695 MI0017328 (SEQ ID NO: 2100) 
                   
               
               
                 CCUGCAGGAGGCAGUGGGCGAGCAGGCGGGGCAGCCCAAUGCCAUGGGCCUGAUCUCACCGCUGCCUCCUUCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4696 MI0017329 (SEQ ID NO: 2101) 
                   
               
               
                 CAAAGCCACUGCAAGACGGAUACUGUCAUCUAUUCCAGAAGAUGACAAUGUCCAUUUUGCAGUGGCUUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4697 MI0017330 (SEQ ID NO: 2102) 
                   
               
               
                 GGGCCCAGAAGGGGGCGCAGUCACUGACGUGAAGGGACCACAUCCCGCUUCAUGUCAGUGACUCCUGCCCCUUGGUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4698 MI0017331 (SEQ ID NO: 2103) 
                   
               
               
                 UGCUUCUCCUGGGGUCUUCCUCUACAUUUCCACCUAGACGGGCCUGGGUCAAAAUGUAGAGGAAGACCCCAGAAGGA 
                   
               
               
                 GCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4699 MI0017332 (SEQ ID NO: 2104) 
                   
               
               
                 AGCAAUUGGAGAAGAUUGCAGAGUAAGUUCCUGAUUAAGAAAUGGAAUUUACUCUGCAAUCUUCUCCAAUUGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4700 MI0017333 (SEQ ID NO: 2105) 
                   
               
               
                 UCAGUGAGGUCUGGGGAUGAGGACAGUGUGUCCUGAAAUUCACAGGACUGACUCCUCACCCCAGUGCACGAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4701 MI0017334 (SEQ ID NO: 2106) 
                   
               
               
                 CCUUGGCCACCACACCUACCCCUUGUGAAUGUCGGGCAAUGGGUGAUGGGUGUGGUGUCCACA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4703 MI0017336 (SEQ ID NO: 2107) 
                   
               
               
                 UUAUGCAUAUUAGCAAUACAGUACAAAUAUAGUGUGUUUGAUUUGCACUGUAGUUGUAUUGUAUUGCCACUCUGUAU 
                   
               
               
                 AA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4704 MI0017337 (SEQ ID NO: 2108) 
                   
               
               
                 CUUAUCCUAGACACUAGGCAUGUGAGUGAUUGUCUUCCUCACUCAAUCAGUCACAUAUCUAGUGUCUAGAAUGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4705 MI0017338 (SEQ ID NO: 2109) 
                   
               
               
                 CUCACAAGAUCAAUCACUUGGUAAUUGCUGUGAUAACAACUCAGCAAUUACCAAGUGAUUGGUUUUGUGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4706 MI0017339 (SEQ ID NO: 2110) 
                   
               
               
                 GCUACGGGGAGCGGGGAGGAAGUGGGCGCUGCUUCUGCGUUAUCUGGAAGGAGCAGCCCACUCCUGUCCUGGGCUCU 
                   
               
               
                 GUGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4707 MI0017340 (SEQ ID NO: 2111) 
                   
               
               
                 GGUUCCGGAGCCCCGGCGCGGGCGGGUUCUGGGGUGUAGACGCUGCUGGCCAGCCCGCCCCAGCCGAGGUUCUCGGC 
                   
               
               
                 ACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4708 MI0017341 (SEQ ID NO: 2112) 
                   
               
               
                 UUUAGGAGAGAGAUGCCGCCUUGCUCCUUGAACAGGAGGAGCAAGGCGGCAUCUCUCUGAUACUAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4709 MI0017342 (SEQ ID NO: 2113) 
                   
               
               
                 CUGCUUCAACAACAGUGACUUGCUCUCCAAUGGUAUCCAGUGAUUCGUUGAAGAGGAGGUGCUCUGUAGCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4710 MI0017344 (SEQ ID NO: 2114) 
                   
               
               
                 GACCGAGUGGGGUGAGGGCAGGUGGUUCUUCCCGAAGCAGCUCUCGCCUCUUCGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4711 MI0017345 (SEQ ID NO: 2115) 
                   
               
               
                 AAAUGUGCAUCAGGCCAGAAGACAUGAGCCCUUUGGAAAGGUCUCGUGUCUUCUGGCUUGAUGCACAUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4712 MI0017346 (SEQ ID NO: 2116) 
                   
               
               
                 GACAGGAUUCCAGUACAGGUCUCUCAUUUCCUUCAUGAUUAGGAAUACUACUUUGAAAUGAGAGACCUGUACUGUAU 
                   
               
               
                 CUGUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4713 MI0017347 (SEQ ID NO: 2117) 
                   
               
               
                 GUCCCCAUUUUUCUCCCACUACCAGGCUCCCAUAAGGGUCGAAUGGGAUCCAGACAGUGGGAGAAAAAUGGGGAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4714 MI0017348 (SEQ ID NO: 2118) 
                   
               
               
                 AUUUUGGCCAACUCUGACCCCUUAGGUUGAUGUCAGAAUGAGGUGUACCAACCUAGGUGGUCAGAGUUGGCCAAAAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4715 MI0017349 (SEQ ID NO: 2119) 
                   
               
               
                 GGGGAAUGAAAGUUGGCUGCAGUUAAGGUGGCUAAUCAGCUGAUGGUGCCACCUUAACUGCAGCCAAUUCUAAUUCC 
                   
               
               
                 CC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4716 MI0017350 (SEQ ID NO: 2120) 
                   
               
               
                 CAUACUUUGUCUCCAUGUUUCCUUCCCCCUUCUGUAUACAUGUAUACAGGAGGAAGGGGGAAGGAAACAUGGAGACA 
                   
               
               
                 AAGUGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4717 MI0017352 (SEQ ID NO: 2121) 
                   
               
               
                 GGCAGUGUUUAGGCCACAGCCACCCAUGUGUAGGGGUGGCUACACAUGGGUGGCUGUGGCCUAAACACUGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4718 MI0017353 (SEQ ID NO: 2122) 
                   
               
               
                 AGCUGUACCUGAAACCAAGCACCUGUUUGUGACUUGGCUUCAGUUACUAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4719 MI0017354 (SEQ ID NO: 2123) 
                   
               
               
                 ACAAUGAUGACUUGUAUGUUAUAGAUUUGUGAUUACAUUAAAACUUAAAAUUUCACAAAUCUAUAAUAUGCAGGUCA 
                   
               
               
                 UCACUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4720 MI0017355 (SEQ ID NO: 2124) 
                   
               
               
                 AAGCCUGGCAUAUUUGGUAUAACUUAAGCACCAGGUAAAAUCUGGUGCUUAAGUUGUACCAAGUAUAGCCAAGUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4721 MI0017356 (SEQ ID NO: 2125) 
                   
               
               
                 GGGCCUGGUCAUGGUCAAGCCAGGUUCCAUCAAGCCCCACCAGAAGGUGGAGGCCCAGGUGAGGGCUCCAGGUGACG 
                   
               
               
                 GUGGGCAGGGUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4722 MI0017357 (SEQ ID NO: 2126) 
                   
               
               
                 GGCAGGAGGGCUGUGCCAGGUUGGCUGGGCCAGGCCUGACCUGCCAGCACCUCCCUGCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4723 MI0017359 (SEQ ID NO: 2127) 
                   
               
               
                 AGUUGGUGGGGGAGCCAUGAGAUAAGAGCACCUCCUAGAGAAUGUUGAACUAAAGGUGCCCUCUCUGGCUCCUCCCC 
                   
               
               
                 AAAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4724 MI0017361 (SEQ ID NO: 2128) 
                   
               
               
                 ACGCAAAAUGAACUGAACCAGGAGUGAGCUUCGUGUACAUUAUCUAUUAGAAAAUGAAGUACCUUCUGGUUCAGCUA 
                   
               
               
                 GUCCCUGUGCGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4725 MI0017362 (SEQ ID NO: 2129) 
                   
               
               
                 GUGUCUCUCUGGAGACCCUGCAGCCUUCCCACCCACCAGGGAGCUUUCCAUGGGCUGUGGGGAAGGCGUCAGUGUCG 
                   
               
               
                 GGUGAGGGAACAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4726 MI0017363 (SEQ ID NO: 2130) 
                   
               
               
                 AGGGCCAGAGGAGCCUGGAGUGGUCGGGUCGACUGAACCCAGGUUCCCUCUGGCCGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4727 MI0017364 (SEQ ID NO: 2131) 
                   
               
               
                 AAUCUGCCAGCUUCCACAGUGGCAGAUUUUCCCAUAGUGGGAAGCUGGCAGAUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4728 MI0017365 (SEQ ID NO: 2132) 
                   
               
               
                 GUGGGAGGGGAGAGGCAGCAAGCACACAGGGCCUGGGACUAGCAUGCUGACCUCCCUCCUGCCCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4729 MI0017366 (SEQ ID NO: 2133) 
                   
               
               
                 UCUGUUUCCUCAUUUAUCUGUUGGGAAGCUAACUGUGACCUUAGCGUCCCAGCAGAUAAAUGAGGAAACAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4730 MI0017367 (SEQ ID NO: 2134) 
                   
               
               
                 CGCAGGCCUCUGGCGGAGCCCAUUCCAUGCCAGAUGCUGAGCGAUGGCUGGUGUGUGCUGCUCCACAGGCCUGGUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4731 MI0017368 (SEQ ID NO: 2135) 
                   
               
               
                 CCCUGCCAGUGCUGGGGGCCACAUGAGUGUGCAGUCAUCCACACACAAGUGGCCCCCAACACUGGCAGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4732 MI0017369 (SEQ ID NO: 2136) 
                   
               
               
                 GAGGGAGCUGUAGAGCAGGGAGCAGGAAGCUGUGUGUGUCCAGCCCUGACCUGUCCUGUUCUGCCCCCAGCCCCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4733 MI0017370 (SEQ ID NO: 2137) 
                   
               
               
                 GGUCGCUUAAAUCCCAAUGCUAGACCCGGUGGCAAUCAAGGUCUAGCCACCAGGUCUAGCAUUGGGAUUUAAGCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4734 MI0017371 (SEQ ID NO: 2138) 
                   
               
               
                 CUCGGGCCCGACCGCGCCGGCCCGCACCUCCCGGCCCGGAGCUGCGGGCUGCGGUCAGGGCGAUCCCGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4735 MI0017372 (SEQ ID NO: 2139) 
                   
               
               
                 UGCAGUGCCUAAUUUGAACACCUUCGGUAUUCAUCAAAAAUACCAAAGGUGCUCAAAUUAGACAUUGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4736 MI0017373 (SEQ ID NO: 2140) 
                   
               
               
                 AGGCAGGUUAUCUGGGCUGCCAUCUCCCACUGGCUGCUUGCCUGCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4737 MI0017374 (SEQ ID NO: 2141) 
                   
               
               
                 CUGCACAGGAUGCGAGGAUGCUGACAGUGCCUCACAGCCGCACAGGACCGAGGAUGCUGACGGUGCCUCACAGCCAC 
                   
               
               
                 ACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4738 MI0017376 (SEQ ID NO: 2142) 
                   
               
               
                 GGUCGCAUUUCUCCUUCUUACCAGCGCGUUUUCAGUUUCAUAGGGAAGCCUUUCCAUGAAACUGGAGCGCCUGGAGG 
                   
               
               
                 AGAAGGGGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4739 MI0017377 (SEQ ID NO: 2143) 
                   
               
               
                 GGGAGGAAGAAGGGAGGAGGAGCGGAGGGGCCCUUGUCUUCCCAGAGCCUCUCCCUUCCUCCCCUCCCCCUCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4740 MI0017378 (SEQ ID NO: 2144) 
                   
               
               
                 GCCAAGGACUGAUCCUCUCGGGCAGGGAGUCAGAGGGGACCGCCCGAGAGGAUCCGUCCCUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4741 MI0017379 (SEQ ID NO: 2145) 
                   
               
               
                 CGGGCGGGGCGGGUCCGGCCGCCUCCGAGCCCGGCCGGCAGCCCCCGGCCUUAAAGCGCGGGCUGUCCGGAGGGGUC 
                   
               
               
                 GGCUUUCCCACCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4742 MI0017380 (SEQ ID NO: 2146) 
                   
               
               
                 UCAGGCAAAGGGAUAUUUACAGAUACUUUUUAAAAUUUGUUUGAGUUGAGGCAGAUUAAAUAUCUGUAUUCUCCUUU 
                   
               
               
                 GCCUGCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4743 MI0017381 (SEQ ID NO: 2147) 
                   
               
               
                 GCUGGCCGGAUGGGACAGGAGGCAUGAAUGAGCCAUCUUUCCAAUGCCUUUCUGUCUUUUCUGGUCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4744 MI0017382 (SEQ ID NO: 2148) 
                   
               
               
                 GUAAUCACAUCUAAAGACUAGACUUCGCUAUGACCAGGCCAUAGUAAACAUCAUAGUAUGUCUAGUCUUUAGGUUUG 
                   
               
               
                 AUUAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4745 MI0017384 (SEQ ID NO: 2149) 
                   
               
               
                 GUGAGUGGGGCUCCCGGGACGGCGCCCGCCCUGGCCCUGGCCCGGCGACGUCUCACGGUCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4746 MI0017385 (SEQ ID NO: 2150) 
                   
               
               
                 GUGUCUGUGCCGGUCCCAGGAGAACCUGCAGAGGCAUCGGGUCAGCGGUGCUCCUGCGGGCCGACACUCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4747 MI0017386 (SEQ ID NO: 2151) 
                   
               
               
                 AGGGAAGGAGGCUUGGUCUUAGCACGGGGUCUAAGGCCCGGGCUUUCCUCCCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4748 MI0017387 (SEQ ID NO: 2152) 
                   
               
               
                 UGGCUGGCUGAGGUUUGGGGAGGAUUUGCUGGUGCUAGAGAGGAAAGCAGACCCUACCCAACCCCACGCCCUACUAC 
                   
               
               
                 AGCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4749 MI0017388 (SEQ ID NO: 2153) 
                   
               
               
                 CCUGCGGGGACAGGCCAGGGCAUCUAGGCUGUGCACAGUGACGCCCCUCCUGCCCCCACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4750 MI0017389 (SEQ ID NO: 2154) 
                   
               
               
                 CGCUCGGGCGGAGGUGGUUGAGUGCCGACUGGCGCCUGACCCACCCCCUCCCGCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4751 MI0017390 (SEQ ID NO: 2155) 
                   
               
               
                 CCCGGAGCCAGAGGACCCGUAGCUGCUAGAAGGGCAGGGGUGUGGCUUCUGGGGGCUGGUCUUCAGCUCUGGCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4752 MI0017391 (SEQ ID NO: 2156) 
                   
               
               
                 AGUGUCUCCUUGUGGAUCUCAAGGAUGUGCUUCCACAUAGCAGCAUGUUCUUCAGAUGGACAAGGAGACACU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4753 MI0017392 (SEQ ID NO: 2157) 
                   
               
               
                 AUAUCUACACAAGGCCAAAGGAAGAGAACAGAUAUAUCCACAGUACACUUGGCUGUUCUCUUUCUUUAGCCUUGUGU 
                   
               
               
                 AGAUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4754 MI0017394 (SEQ ID NO: 2158) 
                   
               
               
                 ACGCGCCUGAUGCGGACCUGGGUUAGCGGAGUGAGGCCCAGUGGUCACCGCCGCCCUCCGCAGGUCCAGGUUGCCGU 
                   
               
               
                 GCGCAUGUGCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4755 MI0017395 (SEQ ID NO: 2159) 
                   
               
               
                 AGAUUCAGCUUUCCCUUCAGAGCCUGGCUUUGGCAUCUAUGAAAGCCAGGCUCUGAAGGGAAAGUUGAAUCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4756 MI0017397 (SEQ ID NO: 2160) 
                   
               
               
                 GGGAUAAAAUGCAGGGAGGCGCUCACUCUCUGCUGCCGAUUCUGCACCAGAGAUGGUUGCCUUCCUAUAUUUUGUGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4757 MI0017398 (SEQ ID NO: 2161) 
                   
               
               
                 UUCCAGCCCGAGGCCUCUGUGACGUCACGGUGUCUGCGGGAGGAGACCAUGACGUCACAGAGGCUUCGCGCUCUGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4758 MI0017399 (SEQ ID NO: 2162) 
                   
               
               
                 GGUGAGUGGGAGCCGGUGGGGCUGGAGUAAGGGCACGCCCGGGGCUGCCCCACCUGCUGACCACCCUCCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4759 MI0017400 (SEQ ID NO: 2163) 
                   
               
               
                 CAUUUAGGACUAGAUGUUGGAAUUAGACAGAAAAAAGUUAGACACAAAAAAUUGUGUCUAAUUCCAACAUCUAGUCC 
                   
               
               
                 UAAAUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4760 MI0017401 (SEQ ID NO: 2164) 
                   
               
               
                 GCCAUGGUGUUUAGAUUGAACAUGAAGUUAGAAUUCUUAAGUAUCAAAACUAAAUUCAUGUUCAAUCUAAACCCCAU 
                   
               
               
                 GGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4761 MI0017402 (SEQ ID NO: 2165) 
                   
               
               
                 GGACAAGGUGUGCAUGCCUGACCCGUUGUCAGACCUGGAAAAAGGGCCGGCUGUGGGCAGGGAGGGCAUGCGCACUU 
                   
               
               
                 UGUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4762 MI0017403 (SEQ ID NO: 2166) 
                   
               
               
                 CUGAUACCCCAAAUCUUGAUCAGAAGCCUUGAUCAGAAGCUAGGAAGGCUUCUGAUCAAGAUUUGUGGUGUCAAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4763 MI0017404 (SEQ ID NO: 2167) 
                   
               
               
                 CCUGUCCCUCCUGCCCUGCGCCUGCCCAGCCCUCCUGCUCUGGUGACUGAGGACCGCCAGGCAGGGGCUGGUGCUGG 
                   
               
               
                 GCGGGGGGCGGCGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4764 MI0017405 (SEQ ID NO: 2168) 
                   
               
               
                 UCUUCCCCAUGGAUGUGGAAGGAGUUAUCUGUCACCAGUCAGAUAACUGUCACCAGUCAGUUAACUCCUUUCACACC 
                   
               
               
                 CAUGGGGAAGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4765 MI0017406 (SEQ ID NO: 2169) 
                   
               
               
                 UGGUGAUUUUGAACGUAGCUAUCCACCACUCAGCCUGGAAAAAGCUGAGUGAUUGAUAGCUAUGUUCAAAAUCACCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4766 MI0017407 (SEQ ID NO: 2170) 
                   
               
               
                 CUGAAGCUCCUUCUGAAAGAGCAGUUGGUGUUUAUUUUUUACUAAAUAGCAAUUGCUCUUUUGGAAGGAACUUGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4767 MI0017408 (SEQ ID NO: 2171) 
                   
               
               
                 ACAUGGGCCCGCGGGCGCUCCUGGCCGCCGCCCGACUUCGGGGCCAGCCGGGGGCAGAGCGCGCGGGAGCCCGAGCGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4768 MI0017409 (SEQ ID NO: 2172) 
                   
               
               
                 AAACUUUGAUUCUCUCUGGAUCCCAUGGAUAUGGGAACUGUGAUGUCCAGGAGAUCCAGAGAGAAUCAGAGUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4769 MI0017410 (SEQ ID NO: 2173) 
                   
               
               
                 GAGGAGAGGUGGGAUGGAGAGAAGGUAUGAGCUAAAAAUCCCCAAGCUCUGCCAUCCUCCCUCCCCUACUUCUCCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4770 MI0017411 (SEQ ID NO: 2174) 
                   
               
               
                 GAGUUAUGGGGUCAUCUAUCCUUCCCUUGGAAAAUGAUCUGAGAUGACACUGUAGCUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4771-1 MI0017412 (SEQ ID NO: 2175) 
                   
               
               
                 GCUCUAGCCUAAUUUUAGAUCUGGUCUGCUUCAGUUUCACUCCAAGCAGACUUGACCUACAAUUAGCCUAGAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4771-2 MI0017413 (SEQ ID NO: 2176) 
                   
               
               
                 GCUCUAGCCUAAUUUUAGAUCUGGUCUGCUUCAGUUUCACUCCAAGCAGACUUGACCUACAAUUAGCCUAGAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4772 MI0017414 (SEQ ID NO: 2177) 
                   
               
               
                 GUGAUUGCCUCUGAUCAGGCAAAAUUGCAGACUGUCUUCCCAAAUAGCCUGCAACUUUGCCUGAUCAGAGGCAGUCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4773-1 MI0017415 (SEQ ID NO: 2178) 
                   
               
               
                 UGCUCCCCAGCCUUUCUAUGCUCCUGUUCUGCUUUAUUUCAUCAAAGCAGAACAGGAGCAUAGAAAGGCUGGGGAGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4773-2 MI0017416 (SEQ ID NO: 2179) 
                   
               
               
                 UGCUCCCCAGCCUUUCUAUGCUCCUGUUCUGCUUUGAUGAAAUAAAGCAGAACAGGAGCAUAGAAAGGCUGGGGAGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4774 MI0017417 (SEQ ID NO: 2180) 
                   
               
               
                 UAUAUUGUUGUCUGGUAUGUAGUAGGUAAUAACUGACAAACAGACAAUUGCCUAACAUGUGCCAGAAAACAACAUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4775 MI0017418 (SEQ ID NO: 2181) 
                   
               
               
                 AUUAAGCUUUUAAUUUUUUGUUUCGGUCACUCUUGAUAGCAGACAUUGACUGAAACAAAAAAUUAAAAGCUUUAU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4776-1 MI0017419 (SEQ ID NO: 2182) 
                   
               
               
                 CUAUAUGCAGUGGACCAGGAUGGCAAGGGCUCUCCUGAAAGGACAGUAGAGCCCUUGCCAUCCUGGUCCACUGCAUA 
                   
               
               
                 UAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4776-2 MI0017420 (SEQ ID NO: 2183) 
                   
               
               
                 CUAUAUGCAGUGGACCAGGAUGGCAAGGGCUCUACUGUCCUUUCAGGAGAGCCCUUGCCAUCCUGGUCCACUGCAUA 
                   
               
               
                 UAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4777 MI0017421 (SEQ ID NO: 2184) 
                   
               
               
                 UAGAAUAUUUCGGCAUUCUAGAUGAGAGAUAUAUAUAUACCUCAUAUGUAUAUGGUAUACCUCAUCUAGAAUGCUGU 
                   
               
               
                 AAUAUUCUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4778 MI0017422 (SEQ ID NO: 2185) 
                   
               
               
                 UCACAUGUCCAAUUCUGUAAAGGAAGAAGAGGUAAGAAGAAGUGAAGCCCUCUUCUUCCUUUGCAGAGUUGAAUAUG 
                   
               
               
                 UGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4779 MI0017423 (SEQ ID NO: 2186) 
                   
               
               
                 UAAAUGUCUUACUGCUUUUACUGUUCCCUCCUAGAGUCCAUUCUUUACUCUAGGAGGGAAUAGUAAAAGCAGUAAGA 
                   
               
               
                 CAUUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4780 MI0017424 (SEQ ID NO: 2187) 
                   
               
               
                 GGCCAGUGCCAGGGGGUCAGGCUCAAGGACCAGCCCAAAGGCCAGGCCUGACCCUUGAGCCUGAUCCCUAGCACUGA 
                   
               
               
                 UCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4781 MI0017426 (SEQ ID NO: 2188) 
                   
               
               
                 AGGUGCACGCUCUAGCGGGGAUUCCAAUAUUGGGCCAAUUCCCCCAAUGUUGGAAUCCUCGCUAGAGCGUGCACUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4782 MI0017427 (SEQ ID NO: 2189) 
                   
               
               
                 AUUGCCCAGUUCUGGAUAUGAAGACAAUCAAGAAAAGAUUUGGUGUUCUUGAUUGUCUUCAUAUCUAGAACUGGGCA 
                   
               
               
                 GU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4783 MI0017428 (SEQ ID NO: 2190) 
                   
               
               
                 GGGAAAGCGGAGGGCGCGCCCAGCUCCCGGGCUGAUUGCGCUAACAGUGGCCCCGGUGUUGGGGCGCGUCUGCCGCU 
                   
               
               
                 GCCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4784 MI0017429 (SEQ ID NO: 2191) 
                   
               
               
                 UGACUGGGCUGAGGAGAUGCUGGGACUGAGAGUGUCAUGGUGGAGCCUCCGUCCCUGCUCAUCCUCUCCGCAUGUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4785 MI0017430 (SEQ ID NO: 2192) 
                   
               
               
                 GUAGGUGGGGACGCGGCGGCGCUGCUCCUCCGCUGCCGCCGGGAGAGUCGGCGACGCCGCCAGCUCCGCGCGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4786 MI0017433 (SEQ ID NO: 2193) 
                   
               
               
                 GGGCAUGGCCUGAGACCAGGACUGGAUGCACCACUCUCCCUGUGAUGAGGUGAAGCCAGCUCUGGUCUGGGCCAUUU 
                   
               
               
                 CAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4787 MI0017434 (SEQ ID NO: 2194) 
                   
               
               
                 CGGUCCAGACGUGGCGGGGGUGGCGGCGGCAUCCCGGACGGCCUGUGAGGGAUGCGCCGCCCACUGCCCCGCGCCGC 
                   
               
               
                 CUGACCG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4788 MI0017435 (SEQ ID NO: 2195) 
                   
               
               
                 AAUGAAGGAUUACGGACCAGCUAAGGGAGGCAUUAGGAUCCUUAUUCUUGCCUCCCUUAGUUGGUCCCUAAUCCUUC 
                   
               
               
                 GUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4789 MI0017436 (SEQ ID NO: 2196) 
                   
               
               
                 CAUGCUACGUAUGUAUACACCUGAUAUGUGUAUGUGUAAAUACAUAUCCACACACAUAGCAGGUGUAUAUAUAGGUA 
                   
               
               
                 GCCUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4790 MI0017437 (SEQ ID NO: 2197) 
                   
               
               
                 CAAUGUGACAUCGCUUUACCAUUCAUGUUCACUGAAAGGUAGAUUUUAAAAACAUGAAUGGUAAAGCGAUGUCACAU 
                   
               
               
                 UG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4791 MI0017438 (SEQ ID NO: 2198) 
                   
               
               
                 UAAGAACUGGAUAUGAUGACUGAAAUAAGCUCCAUAUCAAUGAGAAUUUCAAUGGGAUUAUGUGCAGUCAAUGUCCA 
                   
               
               
                 GUAAUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4792 MI0017439 (SEQ ID NO: 2199) 
                   
               
               
                 GCAGCCCGGUGAGCGCUCGCUGGCCUGGCAGUGCGUCGGAAGAACAGGGCGGGUGGGGCCGCGCACAUCUCUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4793 MI0017440 (SEQ ID NO: 2200) 
                   
               
               
                 UUUCUCCUCGCUGCCCGCACAUCCUGCUCCACAGGGCAGAGGGAGGCCAAGAAGACCUCUGCACUGUGAGUUGGCUG 
                   
               
               
                 GCUGGAGGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4794 MI0017441 (SEQ ID NO: 2201) 
                   
               
               
                 UUUUAACAUCUGGCUAUCUCACGAGACUGUAUGUCCUAACAGUGCUUGUAGUCUCAUGAGAUAGCCAGAUGUUAAAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4795 MI0017442 (SEQ ID NO: 2202) 
                   
               
               
                 UGAUAUGGAAGAAAUCCAGAAGUGGCUAAUAAUAUUGACACUAUAACAAUAAUGUCAAUAUUAUUAGCCACUUCUGG 
                   
               
               
                 AUUUAUGAAUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4796 MI0017443 (SEQ ID NO: 2203) 
                   
               
               
                 UAAAUUUGUGUCUAUACUCUGUCACUUUACUUUUGGCCUCAAGUCAUUGCAGUAAAGUGGCAGAGUAUAGACACAAA 
                   
               
               
                 UUUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4797 MI0017444 (SEQ ID NO: 2204) 
                   
               
               
                 GACUCAGAAGACAGAGUGCCACUUACUGAAAGGUUUUUUCUCUCAGUAAGUGGCACUCUGUCUUCUGAGUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4798 MI0017445 (SEQ ID NO: 2205) 
                   
               
               
                 AAGUACAACUUCGGUAUACUUUGUGAAUUGGCUUUUACAAAAGACCAACUCACGAAGUAUACCGAAGUCAUACUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4799 MI0017446 (SEQ ID NO: 2206) 
                   
               
               
                 ACUGCUAAUAUCUAAAUGCAGCAUGCCAGUCCUGAGAUGCAGGGACUGGCAUGCUGCAUUUAUAUAUUAGCAGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4800 MI0017448 (SEQ ID NO: 2207) 
                   
               
               
                 GGAGAAAGGAGUGGACCGAGGAAGGAAGGAAGGCAAGGCUGUCUGUCCAUCCGUCCGUCUGUCCACCUACCUGUCAG 
                   
               
               
                 UCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4801 MI0017449 (SEQ ID NO: 2208) 
                   
               
               
                 UUGAGGCUUGGUUUUCUUAUGUGUAAAAUGUAAUAACAUUUCUUAUGUUUAAAACACUUUACACAAGAAAACCAAGG 
                   
               
               
                 CUCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4802 MI0017450 (SEQ ID NO: 2209) 
                   
               
               
                 CUGACUGGCUUGUAUGGAGGUUCUAGACCAUGUUAGUGUUCAAGUCUACAUGGAUGGAAACCUUCAAGCAGGCCAAG 
                   
               
               
                 CAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4803 MI0017451 (SEQ ID NO: 2210) 
                   
               
               
                 AGUGGGAUUUAACAUAAUAGUGUGGAUUGAAUCACACACACAUUUCAACCCACACUAUGAUGUUAAAUCCCAUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4804 MI0017452 (SEQ ID NO: 2211) 
                   
               
               
                 UCAGUGUAUUUGGACGGUAAGGUUAAGCAAGGUGCGUCGUAUCUUGCUUAACCUUGCCCUCGAAAUACACUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-4999 MI0017865 (SEQ ID NO: 2212) 
                   
               
               
                 AUAGAAAAUAAAACACAUACUGCUGUAUUGUCAGGUAGUGAUAGGAUUUAUCACUACCUGACAAUACAGUAUGUGUU 
                   
               
               
                 UGUUUUAUAUAUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5000 MI0017866 (SEQ ID NO: 2213) 
                   
               
               
                 CUGAAGAGUAGAGUGUGUGGUCCCAGUUCAGAAGUGUUCCUGAGUAACUUGUGCUUAUAACUCAGGACACUUCUGAA 
                   
               
               
                 CUUGGACCAUACAGGUCUCCCUGCUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5001 MI0017867 (SEQ ID NO: 2214) 
                   
               
               
                 AGCUCAGGGCGGCUGCGCAGAGGGCUGGACUCAGCGGCGGAGCUGGCUGCUGGCCUCAGUUCUGCCUCUGUCCAGGU 
                   
               
               
                 CCUUGUGACCCGCCCGCUCUCCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5002 MI0017868 (SEQ ID NO: 2215) 
                   
               
               
                 UCUUCCUCUCUGUCCUCUGGAAUUUGGUUUCUGAGGCACUUAGUAGGUGAUAGCAUGACUGACUGCCUCACUGACCA 
                   
               
               
                 CUUCCAGAUGAGGGUUACUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5003 MI0017869 (SEQ ID NO: 2216) 
                   
               
               
                 AUGAGUUUGCUUUGUGUCAUCCUCACAACAACCUUGCAGGGUAGAGAUGAUUUUUCCUACUUUUCUAGGUUGUUGGG 
                   
               
               
                 GGCUGGGGCAGGGGGAACAGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5004 MI0017870 (SEQ ID NO: 2217) 
                   
               
               
                 GGCACUUGCUUGGGGGUUAGUGAGGACAGGGCAAAUUCACGAGAUUGGGUUGUGCAGAGGCUGACACUUGGAUUUUC 
                   
               
               
                 CUGGGCCUCAGGACUUCCUUUCAGACAUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5006 MI0017873 (SEQ ID NO: 2218) 
                   
               
               
                 AACCAUUAGGGGGCUGUGGUUUGCCAGGGCAGGAGGUGGAAGGGAGCCCCAUUUACAGUGGUAACUUCCUUUCCCUU 
                   
               
               
                 UCCAUCCUGGCAGGCUUCAGAGAACUUUACCAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5007 MI0017874 (SEQ ID NO: 2219) 
                   
               
               
                 GGUAAACCUUGGUGACUAAUUAGAGUCUGGCUGAUAUGGUUUGACACAGAGCUAAAUCAUAUGAACCAAACUCUAAU 
                   
               
               
                 UAGUCAAUAAUUUCUGUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5008 MI0017876 (SEQ ID NO: 2220) 
                   
               
               
                 GGGCUGACCCCUAGGGUCAGGUGAGGCCCUUGGGGCACAGUGGUGCCAUCUCCCCUGUGCUCCCAGGGCCUCGCCUG 
                   
               
               
                 UCCCUUGAGGUCGGCCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5009 MI0017877 (SEQ ID NO: 2221) 
                   
               
               
                 GACCAGAAGUGUUUUGGAUUUUGGACUUUUUCAGAUUUGGGGAUAUUUGCAUUAUACUUAUCCUAAAUCUGAAAGUC 
                   
               
               
                 CAAAACCUGAAAUGACCAAUAAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5010 MI0017878 (SEQ ID NO: 2222) 
                   
               
               
                 GAUCCAGGGAACCCUAGAGCAGGGGGAUGGCAGAGCAAAAUUCAUGGCCUACAGCUGCCUCUUGCCAAACUGCACUG 
                   
               
               
                 GAUUUUGUGUCUCCCAUUCCCCAGAGCUGUCUGAGGUGCUUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5011 MI0017879 (SEQ ID NO: 2223) 
                   
               
               
                 AGAUGGUAUUGAGUGGAUGCUGUUAUAUAUACAGCCAUGCACUCUGUAGUUUGGGUACACAGUGCAUGGCUGUAUAU 
                   
               
               
                 AUAACACUAUCCAUUCAUCUUUCAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5047 MI0017932 (SEQ ID NO: 2224) 
                   
               
               
                 GAAGCGCUUGCCUAGACGAGACACAGUGCAUAAAAACAACUUUUGGGGGACAGGUAUGUUUUCUUGCAGCUGCGGUU 
                   
               
               
                 GUAAGGUCUUGGCAAGACAAGCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5087 MI0017976 (SEQ ID NO: 2225) 
                   
               
               
                 AGCUUUCUACGGGUUUGUAGCUUUGCUGGCAUGUUAAGUGUUGUCCUACAGUCGCAAGCAUAAGAAAGAGAAAGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5088 MI0017977 (SEQ ID NO: 2226) 
                   
               
               
                 UCUCCAGGGUGGCCAGGCGGGGCCGGGCCUGAGGGAUGGAGGGGAGCCCAUCAGGGCUCAGGGAUUGGAUGGAGGUG 
                   
               
               
                 AUGGGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5089 MI0017978 (SEQ ID NO: 2227) 
                   
               
               
                 AAGGACUUCAGUGGGAUUUCUGAGUAGCAUCCUUGGAAUCUGCACUCAAGGGAUGCUACUCGGAAAUCCCACUGAAG 
                   
               
               
                 UCCUUUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5090 MI0017979 (SEQ ID NO: 2228) 
                   
               
               
                 UCUGAGGUACCCGGGGCAGAUUGGUGUAGGGUGCAAAGCCUGCCCGCCCCCUAAGCCUUCUGCCCCCAACUCCAGCC 
                   
               
               
                 UGUCAGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5091 MI0017980 (SEQ ID NO: 2229) 
                   
               
               
                 GACUGUGGCGACGGAGACGACAAGACUGUGCUGGUCGCGGGUUGUGGGGUUUAGGUCACCGGCAGGGGUCUGGAGUC 
                   
               
               
                 CCUGGAGGUUAGGGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5092 MI0017981 (SEQ ID NO: 2230) 
                   
               
               
                 AUCCCAGAUCAGAUGCCAAAGCCAGUGGGGACUGGACAACAUGAUGAGCCCAAACCAAUCCACGCUGAGCUUGGCAU 
                   
               
               
                 CUGAUUUGGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5093 MI0017982 (SEQ ID NO: 2231) 
                   
               
               
                 CCCGCCAGGUCCACAUGCCAGAGUGUCAACGUGACCCAGCCAGCCUCCUUCCUGAGCUAGGAGGAUUAGGAAAUGAG 
                   
               
               
                 GCUGGCUAGGAGCACAGCCAGGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5094 MI0017983 (SEQ ID NO: 2232) 
                   
               
               
                 AAAAGAAAAAAAUCAGUGAAUGCCUUGAACCUAACACACUGCCUUUUAUGUGGUAGGUACAGUGGGCUCACUGAAAC 
                   
               
               
                 AUUCAACU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5095 MI0018001 (SEQ ID NO: 2233) 
                   
               
               
                 CUGGGAUUACAGGCGUGAACCACCGCGCCCGGCCUAACUUUUAAGAAACGUCGGCCCGGGAGCGGUGGCUCACGCCU 
                   
               
               
                 GUAAUCCCAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5096 MI0018004 (SEQ ID NO: 2234) 
                   
               
               
                 AGUAGAGGUGGGGUUUCACCAUGUUGGUCAGGCUGGUCUCAAACUCCUGACCUCAGGUGAUCCAUCCACC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5100 MI0019116 (SEQ ID NO: 2235) 
                   
               
               
                 CCAUGAGGAGCUGGCAGUGGGAUGGCCUGGGGGUAGGAGCGUGGCUUCUGGAGCUAGACCACAUGGGUUCAGAUCCC 
                   
               
               
                 AGCGGUGCCUCUAACUGGCCACAGGACCUUGGGCAGUCAGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5186 MI0018165 (SEQ ID NO: 2236) 
                   
               
               
                 UCAGCCAGCUUAUGACUUGACCCUCUCACCUGAUUUCUACCAACCUUUCCUCAGCUGAUUUCUUUCUGGGGAGAGAU 
                   
               
               
                 UGGUAGAAAUCAGGUGAGAGGGUCAUGCCAUAAGCUGGCUAAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5187 MI0018166 (SEQ ID NO: 2237) 
                   
               
               
                 GACUAAGGGUGGGAUGAGGGAUUGAAGUGGAGCAGGAAUGCGCUUUUCUCCACUGAAUCCUCUUUUCCUCAGGUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5188 MI0018167 (SEQ ID NO: 2238) 
                   
               
               
                 GGGAGGCAUGGAAAUUUCUCUGGUUUCAAUGGGUACGAUUAUUGUAAGCAGGAUCCAUUCAAUAAUCGGACCCAUUU 
                   
               
               
                 AAACCGGAGAUUUUAAAAGACAGGAAUAGAAUCCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5189 MI0018168 (SEQ ID NO: 2239) 
                   
               
               
                 GGCCCGCCUUUUAGGGGCCUCGCUGUCUGGGCACAGGCGGAUGGACAGGCUGGCCUCUGGAUGACCUGCCAACCGUC 
                   
               
               
                 AGAGCCCAGACCCACGUGGCCUCAGUUGGGGACCAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5190 MI0018169 (SEQ ID NO: 2240) 
                   
               
               
                 GGUCAUACCCUGGCUCCAGCCCUGUCACAUGGUUAAUGUUCCACAGCCAGUGACUGAGCUGGAGCCAGGGCCACUGC 
                   
               
               
                 CCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5191 MI0018170 (SEQ ID NO: 2241) 
                   
               
               
                 AGUUGGCCAGGACCCCAAGCCCCCAGCACUUCAUUCUUGCUGUCCUCUCCUGGUCUGGGAGGAUAGAAGAGAGGAUA 
                   
               
               
                 GGAAGAAUGAAGUGCUGGGCGCUUAGGGGGAUCCUGGCCAACU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5192 MI0018171 (SEQ ID NO: 2242) 
                   
               
               
                 UUAGUUCCAGCCUCCUGGCUCACCUGGAACCAUUUCUCCUGGGAAGCAUGGUAGCCAGGAGAGUGGAUUCCAGGUGG 
                   
               
               
                 UGAGGGCUUGGUACU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5193 MI0018172 (SEQ ID NO: 2243) 
                   
               
               
                 CCUAGGAAAGGCUGCUGGUAACUGGGAUGGGGGUUGGGGGGAGGUAAGAAGUCUCUGACUCCUCCUCUACCUCAUCC 
                   
               
               
                 CAGUUCCAUCACCUGAAGUGGACCUCUUGGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5194 MI0018173 (SEQ ID NO: 2244) 
                   
               
               
                 AUUUCUUUGGGUUAACUUAAACUCAGCCCUUCUAGGCCCAUUCUUUUCACUCAGGAAUUGGAUAAGCUUUUCUGAGG 
                   
               
               
                 GGUUUGGAAUGGGAUGGCAGGGAGAGUCACCAGACACCAUGAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5195 MI0018174 (SEQ ID NO: 2245) 
                   
               
               
                 GAGCAAAAACCAGAGAACAACAUGGGAGCGUUCCUAACCCCUAAGGCAACUGGAUGGGAGACCUGACCCAUCCAGUU 
                   
               
               
                 CUCUGAGGGGGCUCUUGUGUGUUCUACAAGGUUGUUCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5196 MI0018175 (SEQ ID NO: 2246) 
                   
               
               
                 UCUGAGGAGACCUGGGCUGUCAGAGGCCAGGGAAGGGGACGAGGGUUGGGGAACAGGUGGUUAGCACUUCAUCCUCG 
                   
               
               
                 UCUCCCUCCCAGGUUAGAAGGGCCCCCCUCUCUGAAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5197 MI0018176 (SEQ ID NO: 2247) 
                   
               
               
                 UAUGGGAUUCCACAGACAAUGAGUAUCAAUGGCACAAACUCAUUCUUGAAUUUUUGCCAGUUCAAGAAGAGACUGAG 
                   
               
               
                 UCAUCGAAUGCUCUAAAUGUCACUUCACCUCAUGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5571 MI0019115 (SEQ ID NO: 2248) 
                   
               
               
                 AUCUGACACAAAAUGUGAACCAAGCAAUUCUCAAAGGAGCCUCCCAGGAAAUUCACUUUAGGAAGUCCUAGGAGGCU 
                   
               
               
                 CCUCUGAGAGUUGCUAAAACAAAACAUUGAGAGUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5572 MI0019117 (SEQ ID NO: 2249) 
                   
               
               
                 AGCCAGACAAGAGGGUCAUGGGGAGUCACUGUCAACCCAGAGCAGGCACUGCCCCUGCGACCAGCCUGGGGCAUCGG 
                   
               
               
                 UUGGGGUGCAGGGGUCUGCUGGUGAUGCUUUCCAUCUCUUUGCUUUGUCCUGAUUGUAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5579 MI0019133 (SEQ ID NO: 2250) 
                   
               
               
                 UAUGGUACUCCUUAAGCUAACAGGCCCCUGUCACCAUUAGCUUAAGGAGUACCAGAUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5580 MI0019135 (SEQ ID NO: 2251) 
                   
               
               
                 UGCUGGCUCAUUUCAUAUGUGUGCUGAGAAAAUUCACACAUAUGAAGUGAGCCAGCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5581 MI0019136 (SEQ ID NO: 2252) 
                   
               
               
                 AGCCUUCCAGGAGAAAUGGAGACCCUAUACAUACCUGUUUCCAUGCCUCCUAGAAGUUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5582 MI0019138 (SEQ ID NO: 2253) 
                   
               
               
                 UAGGCACACUUAAAGUUAUAGCUACAUCAGUUAUAACUAUAUCAGUUAAAACUUUAAGUGUGCCUAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5583-1 MI0019139 (SEQ ID NO: 2254) 
                   
               
               
                 AAACUAAUAUACCCAUAUUCUGGCUAGGUGAUCAUCAGAAUAUGGGUAUAUUAGUUUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5583-2 MI0019140 (SEQ ID NO: 2255) 
                   
               
               
                 AAACUAAUAUACCCAUAUUCUGAUGAUCACCUAGCCAGAAUAUGGGUAUAUUAGUUUGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5584 MI0019141 (SEQ ID NO: 2256) 
                   
               
               
                 CAGGGAAAUGGGAAGAACUAGAUUUGAAUCCAGACCUUUAGUUCUUCCCUUUGCCCAAUU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5585 MI0019142 (SEQ ID NO: 2257) 
                   
               
               
                 UGAAGUACCAGCUACUCGAGAGGUCAGAGGAUUGCUCCUGAAUAGCUGGGACUACAGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5586 MI0019143 (SEQ ID NO: 2258) 
                   
               
               
                 UAUCCAGCUUGUUACUAUAUGCUUUUUAAAUGGGGCACAGAGUGACAAGCUGGUUAAAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5587 MI0019144 (SEQ ID NO: 2259) 
                   
               
               
                 AUGGUCACCUCCGGGACUCAGCCCUGUGCUGAGCCCCGGGCAGUGUGAUCAUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5588 MI0019147 (SEQ ID NO: 2260) 
                   
               
               
                 ACUGGCAUUAGUGGGACUUUUUUUUUUUUUUUUUUUAAUGUUAAAAGUCCCACUAAUGCCAGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5589 MI0019148 (SEQ ID NO: 2261) 
                   
               
               
                 GGCUGGGUGCUCUUGUGCAGUGAGCAACCUACACAACUGCACAUGGCAACCUAGCUCCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5590 MI0019150 (SEQ ID NO: 2262) 
                   
               
               
                 UUGCCAUACAUAGACUUUAUUGUGUUGAUCAACAAUAAAGUUCAUGUAUGGCAA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5591 MI0019151 (SEQ ID NO: 2263) 
                   
               
               
                 UGGGAGCUAAGCUAUGGGUAUACUGAGCUUAUGUAUGCAUCUGCAUACCCAUAGCUUAGCUCCCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5680 MI0019280 (SEQ ID NO: 2264) 
                   
               
               
                 GCAUUGGGUUAGCAGGUUAGCCCAGCAUUUCCCUUCCUGGACACACAGGAGGAGAAAUGCUGGACUAAUCUGCUAAU 
                   
               
               
                 CCAAUGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5681a MI0019281 (SEQ ID NO: 2265) 
                   
               
               
                 AGUUUUUGAAGAGUAUUGCCACCCUUUCUAGUCCCUAUUAGACUAGAAAGGGUGGCAAUACCUCUUCCAAAAACU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5681b MI0019293 (SEQ ID NO: 2266) 
                   
               
               
                 GAAGAGGUAUUGCCACCCUUUCUAGUCUAAUAGGGACUAGAAAGGGUGGCAAUACUCUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5682 MI0019282 (SEQ ID NO: 2267) 
                   
               
               
                 GGCCCAUGGGUCUUAUCCUGCAAGGUGCUGCAGAGACGAGGCCUGUAGCACCUUGCAGGAUAAGGUCUACUGGGCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5683 MI0019284 (SEQ ID NO: 2268) 
                   
               
               
                 GGAGCUUGUUACAGAUGCAGAUUCUCUGACUUCUUACUGCACCAGUGAAGUCAGGAUCUGCAUUUGAAUAAGACCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5684 MI0019285 (SEQ ID NO: 2269) 
                   
               
               
                 GCUGAACUCUAGCCUGAGCAACAGAGUGAGAUGGUCUUGUUUUGUUGCCCAGGCUGGAGUCCAGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5685 MI0019287 (SEQ ID NO: 2270) 
                   
               
               
                 CUCUACAUCACAGCCCAGCAGUUAUCACGGGCCCCUCCCCUCAAUGGGCCCGUGAUAACUGCAGGGCUGUGAUGUAG 
                   
               
               
                 AG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5686 MI0019290 (SEQ ID NO: 2271) 
                   
               
               
                 UAUCGUAUCGUAUCGUAUCGUAUUGUAUUGUACUGUAUUGUAUUGUACUGUAUUGUAUCGUAUCGUAUCGUAUCGUA 
                   
               
               
                 UCGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5687 MI0019291 (SEQ ID NO: 2272) 
                   
               
               
                 CCUCACUUAUCUGACUCUGAAAUCUUCUAAAUGGUACCCACUUUAUUUAGAACGUUUUAGGGUCAAAUAAGUACAGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5688 MI0019292 (SEQ ID NO: 2273) 
                   
               
               
                 GAAACACUUUGCCUUUUUACAGGAGUUUAUUAUGUUUUGGACAUAGAAACAUAACAAACACCUGUAAAACAGCAAAG 
                   
               
               
                 UGUUUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5689 MI0019294 (SEQ ID NO: 2274) 
                   
               
               
                 AGCGUGGUAGCAUACACCUGUAGUCCUAGAUACUCAGGAGGGUGAGUAUCUAGGACUACAGGUGUGUGCUACCACGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5690 MI0019295 (SEQ ID NO: 2275) 
                   
               
               
                 CUUUUAAUUUCAGCUACUACCUCUAUUAGGAUUUGGGAGUUAUACUAAUAGAGGUAAUAGUUGAAAUUAAGAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5691 MI0019296 (SEQ ID NO: 2276) 
                   
               
               
                 GGACAAGCUUGCUCUGAGCUCCGAGAAAGCUGACAGACAGCUGCUUGGUGUUCAGAGCUUGUCUGUCC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5692a-1 MI0019297 (SEQ ID NO: 2277) 
                   
               
               
                 GACAGUACAAAUAAUACCACAGUGGGUGUACCUCAUGUGUGUACACCCUGUGAUAUUAUUUGUAAUAUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5692a-2 MI0019298 (SEQ ID NO: 2278) 
                   
               
               
                 UACAAAUAAUACCACAGUGGGUGUACCUCAUGUGUGUACACCCUGUGAUAUUAUUUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5692b MI0019311 (SEQ ID NO: 2279) 
                   
               
               
                 GAUAUUAUGAAUAAUAUCACAGUAGGUGUUCACACAUAAUGUGUACACCAUGUGUGUACACCCAUGUGAUAUUUGAA 
                   
               
               
                 GUAGUAUGUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5692c-1 MI0019288 (SEQ ID NO: 2280) 
                   
               
               
                 UAUAACAUUGUAUAUACCCACUGUGAUAUUAAGAGUAAUAGCUCUCUAGGUUAUUAUGAAUAAUAUCACAGUAGGUG 
                   
               
               
                 UACACAAUGUUGUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5692c-2 MI0019289 (SEQ ID NO: 2281) 
                   
               
               
                 UGUGUACACCAACUGUGAUAUUAGGAGUCCUAUUUAUUUUUAGGAUAUUAGGAAUAAUAUCACAGUAGGUGUACACA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5693 MI0019300 (SEQ ID NO: 2282) 
                   
               
               
                 CUGGGAAGUUAGUUCAUUUCAGUCUGUGCUGUGAGCUAGCCAGCAGUGGCUCUGAAAUGAACUCAAACUCUAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5694 MI0019301 (SEQ ID NO: 2283) 
                   
               
               
                 GCCAACUGCAGAUCAUGGGACUGUCUCAGCCCCAUAUGUAUCUGAAGGCUGAGAAGUCCCAUGAUCCGCACUUGGC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5695 MI0019302 (SEQ ID NO: 2284) 
                   
               
               
                 CAAGGCCUAUCUAUCUAGAUUCUUCUUGGCCUCUCUGAGCAUGCAUUCCUGAGACUCCAAGAAGAAUCUAGACAGAU 
                   
               
               
                 AGGCCUUG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5696 MI0019303 (SEQ ID NO: 2285) 
                   
               
               
                 GUGCUCAUUUAAGUAGUCUGAUGCCUACUACUGAUGACAUACAAUGUAAGUGCUCAUUUAGGCGUCAGACUACCUAA 
                   
               
               
                 AUGAGCAC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5697 MI0019304 (SEQ ID NO: 2286) 
                   
               
               
                 AGCAUAUUCUCAAGUAGUUUCAUGAUAAAGGGUGUAUGAGAGAUCAACCCUUUAUCAUGAAACGCUUGAGGAUACGCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5698 MI0019305 (SEQ ID NO: 2287) 
                   
               
               
                 CUGUGCACCUGGGGGAGUGCAGUGAUUGUGGAAUGCAAAGUCCCACAAUCACUGUACUCCCCAGGUGCACAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5699 MI0019306 (SEQ ID NO: 2288) 
                   
               
               
                 CUGUACCCCUGCCCCAACAAGGAAGGACAAGAGGUGUGAGCCACACACACGCCUGGCCUCCUGUCUUUCCUUGUUGG 
                   
               
               
                 AGCAGGGAUGUAG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5700 MI0019307 (SEQ ID NO: 2289) 
                   
               
               
                 UUAAUUAAUGCAUUAAAUUAUUGAAGGCCCUUGGGCACCCCAGGCCUUCAAUAAUUUAAUGCAUUUAUUGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5701-1 MI0019308 (SEQ ID NO: 2290) 
                   
               
               
                 GAUUGGACUUUAUUGUCACGUUCUGAUUGGUUAGCCUAAGACUUGUUCUGAUCCAAUCAGAACAUGAAAAUAACGUC 
                   
               
               
                 CAAUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5701-2 MI0019593 (SEQ ID NO: 2291) 
                   
               
               
                 GAUUGGACUUUAUUGUCACGUUCUGAUUGGUUAGCCUAAGACUUGUUCUGAUCCAAUCAGAACAUGAAAAUAACGUC 
                   
               
               
                 CAAUC 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5702 MI0019309 (SEQ ID NO: 2292) 
                   
               
               
                 GCCUCAACUCCUGGGAUAUGUUGCUGAUCCAACCUGAAAUCCUUCUGUAGGUUGAGUCAGCAACAUAUCCCAUGACU 
                   
               
               
                 UUUGGGU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5703 MI0019310 (SEQ ID NO: 2293) 
                   
               
               
                 UUGCCGUCCCCUUCCUCGUCUUUUCCCCUCAGGAGAAGUCGGGAAGGUGGCGGCGG 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5704 MI0019312 (SEQ ID NO: 2294) 
                   
               
               
                 UGAUCUUGUUUAGGCCAUCAUCCCAUUAUGCUAAGUCCAUGGGCAAACAUAACAGGAUGAUGGCCUAAACAAGACCA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5705 MI0019313 (SEQ ID NO: 2295) 
                   
               
               
                 UCCCCAUUUACACAGGCCAUGAGCCCCGAAACACCCAUCCCAGGAUUGCUGAUGGGUGUUUCGGGGCUCAUGGCCUG 
                   
               
               
                 UGUAAAUGGGGA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5706 MI0019314 (SEQ ID NO: 2296) 
                   
               
               
                 AGCUAGGUCUUCUGGAUAACAUGCUGAAGCUUCUACGUCAUUCAGCACUUGCUUCAGCAUGUUUUCCAGAGGAUCUA 
                   
               
               
                 GCU 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5707 MI0019315 (SEQ ID NO: 2297) 
                   
               
               
                 UGUAAGAACACGUUUGAAUGCUGUACAAGGCACAUAUGUGAACAUUGUACCACAUGUACAGCUUUCAAACAUGCUCU 
                   
               
               
                 UAUA 
                   
               
               
                   
               
               
                 &gt;hsa-mir-5708 MI0019316 (SEQ ID NO: 2298) 
                   
               
               
                 AUUACAGACAUGAGCGACUGUGCCUGACCAAAAGUCAACAUUAAACAACAAAUCUUGGCCAGGCACAGUGGCUCAUG 
                   
               
               
                 CCUGUAAU