Patent Publication Number: US-6040430-A

Title: Nucleic acid encoding site specific recombinase and nuclear receptor fusion protein

Description:
SPECIFICATION 
     The present invention relates to the technical field of genetic manipulation, and more specifically, to the use of recombination-mediated DNA rearrangements and to the use of the regulatory potential of nuclear receptors. 
     The use of site-specific recombinases (SSRs) to induce defined rearrangements of DNA has been described in a variety of organisms (1-12). These reports describe the introduction of a DNA construct that contains SSR target sites. Subsequent exposure to the SSR enzyme activity resulted in the DNA rearrangement determined by the disposition of the target sites (see reference 13 for a recent review of SSRs). Three SSRs have been used in this manner to date; FLP recombinase from the 2 μ episome of Saccharomyces cerevisiae (1,2,5,6,9,10), CRE recombinase from the Escherichia coli phage P1 (3,4,8,11,12) and R recombinase from pSR1 of Zygosaccharomyces rouxii (7). Amongst other SSR systems relevant to the invention described here are those listed in references 13 and 14, and SSRs from Kluyveromyces drosophilarium (15), Kluyveromyces waltii (16), λ Int (17) and the Gin recombination system from phage Mu (18). The content of the above document is incorporated by reference. 
     For many applications in cells, organisms and cell-free in vitro systems, SSR induced DNA rearrangements must be regulated. Current implementation of the potential offered by SSRs is limited by the means available to regulate SSR activity. In experiments with cultured cells, unregulated SSRs have been used. For example, after introduction of SSR target sites into cells, recombination has been induced by subsequent introduction of either FLP recombinase by transfection of DNA (4,9) or injection of CRE recombinase protein (12). That is, the intended recombination event was regulated merely by the time of introduction of an appropriate macromolecule. Amongst other limitations, this precludes the creation and proliferation of homogeneous populations of cells that contain both the unrearranged target sites and the SSR and in which the recombination event can be induced after cell numbers have been expanded. 
     In experiments with transgenic animals, the issue of SSR regulation has been addressed by regulating the expression of an introduced SSR gene using the inducible heat-shock promoter in Drosophila (5) or a tissue-specific promoter in mice (11). Both approaches have limited applicability. Namely, heat-shock regulation of transgene expression is currently only useful in flies and no suitable counterpart is available for use in cell lines or vertebrates. Also, the use of a tissue-specific promoter to regulate transgene expression relies on the limited availability of suitable promoters and enhancers and the expression pattern achieved is confined to the times and places at which these tissue specific elements are active. 
     The problem underlying the present invention was to provide a regulated recombination system, wherein the disadvantages of the prior art are at least partially eliminated. More specifically, the problem was to provide a recombination system, wherein the recombination event can be induced independently from any tissue specific restrictions. 
     This patent application describes an invention that regulates SSR activity, rather than its expression. Thus any means of achieving and directing expression can be used, such as using ubiquitous or broadly active promoters and enhancers, as well as tissue specific or inducible promoters and enhancers. 
     One aspect of the present invention relates to a fusion protein, comprising a recombinase protein or a component of a recombinase complex, fused to part or all of a nuclear receptor, so that the amino acids that bind the ligand of said nuclear receptor are included, such that in cells or appropriate cell-free systems: (a) recombinase activity is inhibited in the absence of ligand binding to said ligand binding domain and (b) recombinase activity is induced or altered by binding of ligand to said ligand binding domain. 
     Preferably the recombinase activity is altered by binding of ligand to ligand binding domain by a factor of at least 10, more preferably of at least 20 and most preferably of at least 40. 
     The present invention resides in the regulation of SSR activity by fusing an SSR protein to the ligand binding domain (LBD) of a nuclear receptor. SSR-LBD fusion proteins comprise an amino acid sequence of SSRs physically attached to the amino acid sequence of an LBD of a nuclear receptor. That is, the invention is a fusion protein, comprising a recombinase protein, or a component of a recombinase complex, fused to the ligand binding domain of a nuclear receptor. Preferably, the recombinase protein or a component of a recombinase complex is fused to the nuclear receptor or ligand binding domain thereof by means of genetic fusion, i.e. the SSR-LBD protein is a linear genetic fusion encoded by a single nucleic acid. On the other hand, the present invention also encompasses SSR-LBD fusion proteins which are linked by different means, e.g. through a spacer molecule having reactive groups thereon, which are covalently bound to each protein domain. 
     Most simply, the attachment of the SSR and LBD components can be achieved by making a DNA construct that encodes for the amino acid sequence of the SSR-LBD fusion protein with the LBD encoding DNA placed in the same reading frame as the SSR encoding DNA, preferably either at the amino or carboxy termini of the SSR protein (19). More preferably, the nuclear receptor or ligand binding domain thereof is fused to the C-terminus of the recombinase protein or component of a recombinase complex. In an especially preferred embodiment of the present invention the nuclear receptor or ligand binding domain thereof is fused to the recombinase protein or component of a recombinase complex through a peptide linker which mainly consists of hydrophilic acids and preferably has a length of 4 to 20 amino acids. 
     SSR-LBD fusion proteins can coexist with target sites without recombination occurring since these proteins require ligand binding to the LBD for recombinase activity. In our experiments, recombinase activity of the SSR-LBD fusion protein, in the absence of the relevant ligand, is at least 200× less active than wild type recombinase activity. Upon presenting the SSR-LBD fusion proteins with the relevant ligand, recombinase activity is induced to more than 20% of wild type, that is, equal to or greater than 40× induction. This is the first description of post-transcriptional regulation of SSRs by any means. In particular, the invention permits the propagation of both the unrearranged target sites and the SSR-LBD in living systems. More specifically: 
     (a) cell lines can be established that carry both the unrearranged target sites and the SSR-LBD as a prerequisite for biochemical studies or introduction into organisms. The cell lines can be homogeneous and characterised before expansion to the required quantities and subsequent induction of recombination by administration of the relevant ligand, 
     (b) recombination can be regulated in any experimentally manipulatable multicellular organism by administration of the relevant ligand. The SSR-LBD could be introduced to the organism either through the incorporation of cells or by direct means such as microinjection or in the genome of a viral based vector. Recombination can be induced after characterisation by administration of the relevant ligand. 
     The term &#34;nuclear receptor&#34;, as used herein, refers to a molecule, preferably a protein molecule, which may be glycosylated or unglycosylated, having the abilities to bind ligand and to be incorporated into a nucleus of a cell. Specifically, the term nuclear receptor refers to those proteins that display functional or biochemical properties that are similar to the functional or biochemical properties displayed by the steroid hormone receptors with respect to ligand binding, for example, the dioxin receptor (Whitelaw, M. L., Gottlicher, M., Gustafsson, J. -A. and Poellinger, L., (1993) EMBO J. 12, 4169-4179). More specifically, the term nuclear receptor refers to those proteins that are related by their amino acid sequence to the LBDs of the steroid hormone receptors. The paper of Laudet et al. (1992) (Laudet, V., Hanni, C., Coll, J., Catzeflis, F. and Stehelin, D. (1992) EMBO J. 11, 1003-1013) describes an alignment of nuclear receptor amino acid sequences using standard methodologies. Included in the definition of nuclear receptors used here are also those proteins not listed in Laudet et al. but which can be included using the methods employed by Laudet et al. The term nuclear receptor also includes mutant derivatives of nuclear receptor amino acid sequences which retain sufficient relatedness to nuclear receptor amino acid sequences as to be identifiable as related using the methods employed by Laudet et al. 
     The nuclear receptor which is fused to the recombinase protein is preferably a hormone receptor, e.g. a receptor recognized by steroids, vitamins or related ligands. Examples of suitable nuclear receptors are listed in reference 20, which is hereby incorporated by reference. Preferably, the nuclear receptor is a steroid hormone receptor, more preferably, a glucocorticoid, estrogen, progesteron, or androgen receptor. 
     In the SSR-LBD fusion protein of the present invention, it is not required that the complete nuclear receptor is present; i.e. it is sufficient that the amino acids that bind the ligand are fused to the SSR. 
     Upon binding their relevant ligand, nuclear receptors become active, or altered, transcription factors. The cloning of cDNAs encoding members of the steroid receptor family of proteins revealed that they share amino acid sequence homology, in particular in the protein domain that binds ligand. The LBD can be separated from the rest of the protein and fused to other transcription factors conferring ligand regulation onto the resulting fusion proteins. For the glucocorticoid and estrogen receptors, the domain that binds ligand has been fused to other transcription factors and also to oncoproteins, rendering the fusion proteins dependent on the relevant ligand for activity (19,21-26). All characterised oncoproteins that have been experimentally regulated in this manner so far are also transcription factors. Transcription factors and oncoproteins are not enzymes in the classical sense and the regulation of their activities by steroids and LBDs described in references 19, 21-26, is essentially similar to the role that LBDs play in regulating steroid receptors. Prior attempts to extend the regulatory possibilities of the LBD fusion protein strategy beyond transcription factors and oncoproteins have yet to be reported. Attempts to regulate the enzyme activities of either β-galactosidase or dihydrofolate reductase by fusing them with the glucocorticoid LBD have been unsuccessful (D. Picard, public seminar given at EMBL, Heidelberg, Nov. 1991). 
     The present invention extends the regulatory possibilities of the LBD fusion protein strategy beyond the currently documented transference of ligand regulation from nuclear receptors to other transcription factors, to include an enzyme activity, namely a site specific recombinase. The term &#34;site specific recombinase&#34; refers to any protein component of any recombinant system that mediates DNA rearrangements in a specific DNA locus, including site specific recombinases of the integrase or resolvase/invertase classes (13; Abremski, K. E. and Hoess, R. H. (1992) Protein Engineering 5, 87-91; Khan, E., Mack, J. P. G., Katz, R. A., Kulkosky, J. and Skalka, A. M. (1991) Nucleic acids Res. 19, 851-860) and site-specific recombination mediated by intron-encoded endonucleases (Perrin, A., Buckle, M. and Dujon, B. (1993) EMBO J. 12, 2939-2947). Preferred recombinase proteins, which can be used in the fusion proteins according to the invention, are selected from the group consisting of: FLP recombinase, Cre recombinase, R recombinase from the Zygosaccharomyces rouxii plasmid PSR1, A recombinase from the Kluyveromyces drosophilarium plasmid pKD1, A recombinase from the Kluyveromyces waltii plasmid pKW1, any component of the λ Int recombination system, any component of the Gin recombination system, or variants thereof. The term &#34;variant&#34; in this context refers to proteins which are derived from the above proteins by deletion, substitution and/or addition of amino acids and which retain their some or all of the function inherent in the protein from which they are derived. Specifically, the variant could retain the ability to act as a recombinase, or it could retain protein/protein or protein/DNA interactions critical to the recombination reaction, or to the regulation of the recombination reaction. 
     In a preferred embodiment of the invention, FLP recombinase is fused to the LBD of the estrogen, glucocorticoid, progesterone or androgen receptors (20). Other preferred embodiments include fusing Cre recombinase or R recombinase, or SSRs from Kluyveromyces drosophilarium or Kluyveromyces waltii, to these LBDs. Another preferred embodiment involves regulating one or more components of an SSR complex to these LBDs, in particular, components of the λ Int or Gin recombination systems. The invention, in providing a means to regulate recombination, is however not limited to known recombinases and recombination complexes and is not limited to known nuclear receptor LBDs. Rather, the strategy of fusing recombinases, or components of recombination complexes, to LBDs of nuclear receptors is applicable to any fusion combination of these proteins which display the desired characteristics readily identifiable without undue experimentation on the part of a skilled person. 
     A further subject-matter of the present invention is a nucleic acid which encodes the fusion protein according to the present invention. Preferably, the nucleic acid is a DNA or RNA. 
     Still a further subject-matter of the present invention is a recombinant vector comprising at least one copy of the nucleic acid as defined above. This recombinant vector may be a eukaryotic vector, a viral vector, or a prokaryotic vector, or a vector which can be maintained in eukaryotic and prokaryotic host cells. The recombinant vector is obtainable by inserting a nucleic acid encoding a SSR-LBD fusion protein into a suitable starting vector. Specific examples of suitable starting vectors are given for example in Molecular Cloning. A Laboratory Manual, 2nd edition, J. Sambrook et al. (1989), Cold Spring Harbor Laboratory Press, chapters 1, 2, 3, 4, 16 and 17. 
     The vector of the present invention may be an extra-chromosomal vector or a vector which integrates into the genome of the host cell. Extra-chromosomal vectors, e.g. circular plasmids are especially preferred. An example of a plasmid vector is the plasmid pHFE1 comprising a nucleic acid encoding a fusion of the FLP recombinase and the ligand binding domain of the estrogen receptor. The plasmid PHFE1 has been deposited at the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM), Mascheroder Weg 1b, D-38124 Braunschweig on Jun. 25, 1994 under the accession number DSM 9265 according to the requirements of the Budapest Treaty. 
     Still a further subject-matter of the present invention is a cell containing a nucleic acid or a recombinant vector as defined above. Preferably, the cell is stably transformed with the nucleic acid or the vector. Suitable cells are eukaryotic or prokaryotic cells. Examples of prokaryotic cells are gram-negative bacterial cells, especially E. coli cells. Examples of eukaryotic cells are mammalian cells, yeasts and trypanosomes. 
     The invention separates the function of ligand binding from the functions of transcription factors, coupling ligand binding to recombinase activity. Thereby, the binding of ligand can be assessed by any means that measures recombinase activity. Thus, further embodiments of the invention include methods for determining the binding of ligand to the LED of a nuclear receptor, comprising the steps of: 
     (i) the introduction of the SSR-LBD fusion protein or the nucleic acid coding therefor into cells, or appropriate cell-free systems, that contain the DNA target sites for the SSR; 
     (ii) administering the ligand or a mixture suspected to contain a ligand or ligands to be evaluated; if the ligand is not already present in the system; (iii) detecting the recombinase activity, if any, of the SSR-LBD by detecting, directly or indirectly, recombination or changes in the recombination rate between the DNA target sites. 
     The introduction of SSR-LBD fusion proteins into cells may be accomplished by transforming the cells with appropriate vectors containing a nucleic acid coding for the SSR-LBD fusion protein. The administration of the ligand to be evaluated is not necessary in systems which already contain the ligand and in which the ligand-concentration is determined by detecting recombination between DNA target sites. 
     Particularly preferred embodiments include: 
     (a) direct measurements of the physical arrangement of the target sites using techniques such as gel electrophoresis of DNA molecules, Southern blotting or polymerase chain reaction. 
     (b) indirect measurements by assessing the properties encoded by the DNA regions carrying the target sites before or after recombination. For example, recombination could excise an antibiotic resistance gene from the genome of the host and thus recombination can be measured as sensitivity to the antibiotic. 
     (c) the measurement of intracellular ligand concentrations by evaluating recombination between the DNA target sites resident in the cells that also carry the SSR-LBD fusion proteins. 
     (d) the evaluation of ligand binding to an LBD without the need to use radiolabelled ligand derivatives or without reliance on the transcription factor properties of the corresponding nuclear receptor. 
     (e) evaluating the effect of mutations in the LBD on ligand binding. Since SSR-LBD recombination requires ligand, mutations in the LBD that decrease ligand binding can be ascertained. Alternatively, mutations that improve binding of a different ligand can be selected. For example, ligand-dependent recombination could rearrange the DNA, so that an antibiotic resistance gene is expressed. Cells with the rearranged DNA will grow under the appropriate antibiotic selection, cells with unrearranged DNA will not. Specifically this describes a method for determining the effect of mutations in the LBD of a nuclear receptor on its ability to bind ligand, comprising the steps of (a) introducing mutations into the LBD of the SSR-LBD fusion protein, and (b) following steps (i) to (iii) above. 
     The invention also encompasses a method for regulating the recombination of DNA target sites, comprising the steps of: 
     (a) providing cells or appropriate cell-free systems that contain DNA target sites for a site-specific recombinase, 
     (b) contacting the DNA target sites with a fusion protein according to the present invention, which contacting may be accomplished by direct introduction of the protein or by transformation of a cell with the nucleic acid encoding the protein and expressing the nucleic acid to produce the protein, 
     (c) effecting a recombination of said DNA target sites by contacting the fusion protein with a ligand for the nuclear receptor component of the fusion protein, e.g. by adding the ligand to the system at a predetermined time, or by effecting production of the ligand in the system at a predetermined time. 
    
    
     DESCRIPTION OF THE FIGURES 
     FIG. 1A depicts the plasmid, pNEOβGAL (thin line), integrated into the genome of 293 cells (thick line). FLP recombinase target sites (FRTs) are depicted as broad arrows and the neomycin resistance gene lies between the two FRTs. Upon estradiol administration, the DNA between the FRTs is excised, leaving one FRT in the genome and one on the excised circular DNA. The probe used for the Southern blot of FIG. 2 is also depicted. 
     FIG. 1B depicts the steps involved in the creation of plasmid pHFE1. 
     FIG. 2 shows a Southern blot of a timecourse of recombination occurring in the cell line P1.4. At the times indicated after estradiol, or ethanol only, administration in the upper part of the figure, cells were lysed, DNA purified, restricted with NdeI, run on a 1.25% agarose gel and blotted to Biodyne B membranes by standard methodologies. The membrane was hybridized with the radioactively labelled probe depicted in FIG. 1A. The upper band shows the unrecombined integrated DNA and the lower the recombined band. Estradiol was dissolved in ethanol and the extreme right hand lane shows cells treated with the equivalent amount of ethanol, without estradiol, for 51 hours. 
     FIG. 3 shows the complete amino acid sequence of the SSR-LBD-fusion polypeptide encoded by the plasmid pHFE1 [SEQ ID NO: 1]. The FLP recombinase domain is located from amino acid 1-423. The linker peptide is located from amino acid 424-428. The estrogen binding domain is located from amino acid 429-773. 
     FIG. 4 shows the nucleic acid sequence coding for the SSR-LBD fusion polypeptide of FIG. 3 [SEQ ID NO: 2]. The parts coding for the FLP recombinase domain, the linker peptide and the estrogen binding domain, respectively, are specified. 
    
    
     The following examples are offered by way of illustration and is not intended to limit the invention in any manner. 
     EXAMPLE 1 
     Construction of Plasmid Containing a SSR-LBD-fusion Gene 
     The experiment involves two plasmids, one of which is the target for recombination, and the other is the recombinase expression plasmid. As the target plasmid, pNEOβGAL (9) was employed. pNEOβGAL contains two FLP recombinase target sites (FRT) surrounding a gene encoding neomycin resistance (FIG. 1a). The expression plasmid, pHFE 1, carries two genes, one encoding an FLP recombinase-estrogen receptor LBD fusion protein and the other encoding hygromycin resistance (FIG. 1b). The plasmid was constructed by standard cloning procedures using pBluescribe (Stratagene), pOG 44 (9), pHE63 (22) and pCNH2 (27). The stop codon of the FLP recombinase coding region present in pOG44 was mutated by oligonucleotide replacement to introduce BamH1, BsiW1 and EcoR1 sites and continue the open reading frame. The BamHI site in the estrogen receptor encoded by pHE63 was used to join the coding regions of FLP recombinase and estrogen receptor LBD. All of the estrogen receptor coding region carboxy to the BamHI site is present in the plasmid, including its stop codon. 
     EXAMPLE 2 
     Regulation of Site Specific Recombination 
     pNEOβGAL was introduced into 293 human embryonal kidney cells by electroporation (5×10 6  cells in 500 μl phosphate-buffered saline with 5 μg of ApaI linearised plasmid; 300 V at 125 μF in a 1 ml cuvette using a Bio-Rad Gene Pulser) and selection for neomycin resistance (G418, 0.4 mg/ml). Single colonies of resistant cells were cloned and characterised for incorporation of target plasmid DNA by Southern blotting. A clone, P1, showing integration of a single copy of target plasmid DNA was selected and transfected, using the calcium phosphate procedure, with the second plasmid, PHFE 1 (1 μg of XmnI linearised pHFE 1 was precipitated by the standard calcium phosphate method onto 10 7  P1 cells which were cultured in DMEM without phenol red, 10% charcoal stripped fetal calf serum). Four clones resistant to both neomycin and hygromycin (0.4 mg/ml G418, 0.4 mg/ml hygromycin B) were isolated and the dependance of recombination on estradiol administration was observed in all four. For clone P1.4, a time course of recombination in the presence of 10 -6  M estradiol is shown (FIG. 2). 
     REFERENCES 
     1. Falco, S. C., Li. Y., Broach, J. R. and Botstein, D. (1982) Cell 29, 573-584. 
     2. Cox, M. M. (1983) Proc. Natl. Acad. Sci. USA 80, 4223-4227. 
     3. Sauer, B. (1987) Mol. Cell. Biol. 7, 2087-2096. 
     4. Sauer, B. and Henderson, N. (1989) Nucleic Acids Res. 17, 147-161. 
     5. Golic, K. G. and Lindquist, S. (1989) Cell 59, 499-509. 
     6. Cregg, J. M. and Madden, K. R. (1989) Mol. Gen. Genet. 219, 320-323. 
     7. Matsuzaki, H. et al. (1990) J. Bacteriol. 172, 610-618. 
     8. Odell, J., Caimi, P., Sauer, B. and Russell, S. (1990) Mol. Gen. Genet. 223, 369-378. 
     9. O&#39;Gorman, S., Fox, D. T. and Wahl, G. M. (1991) Science 251, 1351-1355. 
     10. Konsolaki, M. et al (1992) New Biologist 4, 551-557. 
     11. Lakso, M. et al (1992) Proc. Natl. Acad. Sci. USA 89, 6232-6236. 
     12. Baubonis, W. and Sauer, B. (1993) Nucleic Acids Res. 21, 2025-2029. 
     13. Stark, W. M. et al. (1992) Trends Genet. 8. 432-439. 
     14. Utatsu, I. et al (1987) J. Bacteriol. 169, 5537-5545. 
     15. Chen, X. J. et al (1986) Nucleic Acids Res. 314, 4471-4481. 
     16. Chen, Y. J. et al (1992) J. Gen. Microbiol. 138, 337-345. 
     17. Landy, A. (1989) Annu. Rev. Biochem. 58, 913-949. 
     18. Klippel, A. et al (1993) EMBO J. 12, 1047-1057. 
     19. Webster, N. J. G. et al (1988) Cell 54, 194-207 
     20. Beato, M. (1989) Cell 56, 335-344. 
     21. Kumar, V. et al (1987) Cell 51, 941-951. 
     22. Picard, D. Salser, S. J. and Yamamoto, K. R. (1988) Cell 54, 1073-1080. 
     23. Eilers, M., Picard, D., Yamamoto, K. R. and Bishop, M. (1989) Nature 340, 66-68. 
     24. Superti-Furga, G. et al (1991) Proc. Natl. Acad. Sci. USA 88, 5114-5118. 
     25. Burk, O. and Klempenauer, K. -H. (1991) EMBO J. 10, 3713-3719. 
     26. Boehmelt, G. et al (1992) EMBO J. 11, 4641-4652. 
     27. Schmidt, E. et al (1990) Mol. Cell. Biol. 10, 4406-4411. 
     
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#             SEQUENCE LISTING                                            
- (1) GENERAL INFORMATION:                                                
-    (iii) NUMBER OF SEQUENCES: 2                                         
- (2) INFORMATION FOR SEQ ID NO: 1:                                       
-      (i) SEQUENCE CHARACTERISTICS:                                      
#acids    (A) LENGTH: 773 amino                                           
          (B) TYPE: amino acid                                            
          (D) TOPOLOGY: linear                                            
-     (ii) MOLECULE TYPE: protein                                         
-     (ix) FEATURE:                                                       
          (A) NAME/KEY: misc.sub.-- - #feature                            
          (B) LOCATION: (1-423)                                           
#/note= &#34;FLP recombinase domain.&#34;                                         
-     (ix) FEATURE:                                                       
          (A) NAME/KEY: misc.sub.-- - #feature                            
          (B) LOCATION: (424-428)                                         
#/note= &#34;Linker peptide.&#34;MATION:                                          
-     (ix) FEATURE:                                                       
          (A) NAME/KEY: misc.sub.-- - #feature                            
          (B) LOCATION: (429-773)                                         
#/note= &#34;Estrogen binding domain.&#34;                                        
#1:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:                               
- Met Pro Gln Phe Asp Ile Leu Cys Lys Thr Pr - #o Pro Lys Val Leu Val     
#                 15                                                      
- Arg Gln Phe Val Glu Arg Phe Glu Arg Pro Se - #r Gly Glu Lys Ile Ala     
#             30                                                          
- Leu Cys Ala Ala Glu Leu Thr Tyr Leu Cys Tr - #p Met Ile Thr His Asn     
#         45                                                              
- Gly Thr Ala Ile Lys Arg Ala Thr Phe Met Se - #r Tyr Asn Thr Ile Ile     
#     60                                                                  
- Ser Asn Ser Leu Ser Ser Asp Ile Val Asn Ly - #s Ser Leu Gln Phe Lys     
# 80                                                                      
- Tyr Lys Thr Gln Lys Ala Thr Ile Leu Glu Al - #a Ser Leu Lys Lys Leu     
#                 95                                                      
- Ile Pro Ala Trp Glu Phe Thr Ile Ile Pro Ty - #r Tyr Gly Gln Lys His     
#           110                                                           
- Gln Ser Asp Ile Thr Asp Ile Val Ser Ser Le - #u Gln Leu Gln Phe Glu     
#       125                                                               
- Ser Ser Glu Glu Ala Asp Lys Gly Asn Ser Hi - #s Ser Lys Lys Met Leu     
#   140                                                                   
- Lys Ala Leu Leu Ser Glu Gly Glu Ser Ile Tr - #p Glu Ile Thr Glu Lys     
145                 1 - #50                 1 - #55                 1 -   
#60                                                                       
- Ile Leu Asn Ser Phe Glu Tyr Thr Ser Arg Ph - #e Thr Lys Thr Lys Thr     
#               175                                                       
- Leu Tyr Gln Phe Leu Phe Leu Ala Thr Phe Il - #e Asn Cys Gly Arg Phe     
#           190                                                           
- Ser Asp Ile Lys Asn Val Asp Pro Lys Ser Ph - #e Lys Leu Val Gln Asn     
#       205                                                               
- Lys Tyr Leu Gly Val Ile Ile Gln Cys Leu Va - #l Thr Glu Thr Lys Thr     
#   220                                                                   
- Ser Val Ser Arg His Ile Tyr Phe Phe Ser Al - #a Arg Gly Arg Ile Asp     
225                 2 - #30                 2 - #35                 2 -   
#40                                                                       
- Pro Leu Val Tyr Leu Asp Glu Phe Leu Arg As - #n Ser Glu Pro Val Leu     
#               255                                                       
- Lys Arg Val Asn Arg Thr Gly Asn Ser Ser Se - #r Asn Lys Gln Glu Tyr     
#           270                                                           
- Gln Leu Leu Lys Asp Asn Leu Val Arg Ser Ty - #r Asn Lys Ala Leu Lys     
#       285                                                               
- Lys Asn Ala Pro Tyr Ser Ile Phe Ala Ile Ly - #s Asn Gly Pro Lys Ser     
#   300                                                                   
- His Ile Gly Arg His Leu Met Thr Ser Phe Le - #u Ser Met Lys Gly Leu     
305                 3 - #10                 3 - #15                 3 -   
#20                                                                       
- Thr Glu Leu Thr Asn Val Val Gly Asn Trp Se - #r Asp Lys Arg Ala Ser     
#               335                                                       
- Ala Val Ala Arg Thr Thr Tyr Thr His Gln Il - #e Thr Ala Ile Pro Asp     
#           350                                                           
- His Tyr Phe Ala Leu Val Ser Arg Tyr Tyr Al - #a Tyr Asp Pro Ile Ser     
#       365                                                               
- Lys Glu Met Ile Ala Leu Lys Asp Glu Thr As - #n Pro Ile Glu Glu Trp     
#   380                                                                   
- Gln His Ile Glu Gln Leu Lys Gly Ser Ala Gl - #u Gly Ser Ile Arg Tyr     
385                 3 - #90                 3 - #95                 4 -   
#00                                                                       
- Pro Ala Trp Asn Gly Ile Ile Ser Gln Glu Va - #l Leu Asp Tyr Leu Ser     
#               415                                                       
- Ser Tyr Ile Asn Arg Arg Ile Ser Val Arg Gl - #y Ser Met Lys Gly Gly     
#           430                                                           
- Ile Arg Lys Asp Arg Arg Gly Gly Arg Met Le - #u Lys His Lys Arg Gln     
#       445                                                               
- Arg Asp Asp Gly Glu Gly Arg Gly Glu Val Gl - #y Ser Ala Gly Asp Met     
#   460                                                                   
- Arg Ala Ala Asn Leu Trp Pro Ser Pro Leu Me - #t Ile Lys Arg Ser Lys     
465                 4 - #70                 4 - #75                 4 -   
#80                                                                       
- Lys Asn Ser Leu Ala Leu Ser Leu Thr Ala As - #p Gln Met Val Ser Ala     
#               495                                                       
- Leu Leu Asp Ala Glu Pro Pro Ile Leu Tyr Se - #r Glu Tyr Asp Pro Thr     
#           510                                                           
- Arg Pro Phe Ser Glu Ala Ser Met Met Gly Le - #u Leu Thr Asn Leu Ala     
#       525                                                               
- Asp Arg Glu Leu Val His Met Ile Asn Trp Al - #a Lys Arg Val Pro Gly     
#   540                                                                   
- Phe Val Asp Leu Thr Leu His Asp Gln Val Hi - #s Leu Leu Glu Cys Ala     
545                 5 - #50                 5 - #55                 5 -   
#60                                                                       
- Trp Leu Glu Ile Leu Met Ile Gly Leu Val Tr - #p Arg Ser Met Glu His     
#               575                                                       
- Pro Val Lys Leu Leu Phe Ala Pro Asn Leu Le - #u Leu Asp Arg Asn Gln     
#           590                                                           
- Gly Lys Cys Val Glu Gly Met Val Glu Ile Ph - #e Asp Met Leu Leu Ala     
#       605                                                               
- Thr Ser Ser Arg Phe Arg Met Met Asn Leu Gl - #n Gly Glu Glu Phe Val     
#   620                                                                   
- Cys Leu Lys Ser Ile Ile Leu Leu Asn Ser Gl - #y Val Tyr Thr Phe Leu     
625                 6 - #30                 6 - #35                 6 -   
#40                                                                       
- Ser Ser Thr Leu Lys Ser Leu Glu Glu Lys As - #p His Ile His Arg Val     
#               655                                                       
- Leu Asp Lys Ile Thr Asp Thr Leu Ile His Le - #u Met Ala Lys Ala Gly     
#           670                                                           
- Leu Thr Leu Gln Gln Gln His Gln Arg Leu Al - #a Gln Leu Leu Leu Ile     
#       685                                                               
- Leu Ser His Ile Arg His Met Ser Asn Lys Gl - #y Met Glu His Leu Tyr     
#   700                                                                   
- Ser Met Lys Cys Lys Asn Val Val Pro Leu Ty - #r Asp Leu Leu Leu Glu     
705                 7 - #10                 7 - #15                 7 -   
#20                                                                       
- Met Leu Asp Ala His Arg Leu His Ala Pro Th - #r Ser Arg Gly Gly Ala     
#               735                                                       
- Ser Val Glu Glu Thr Asp Gln Ser His Leu Al - #a Thr Ala Gly Ser Thr     
#           750                                                           
- Ser Ser His Ser Leu Gln Lys Tyr Tyr Ile Th - #r Gly Glu Ala Glu Gly     
#       765                                                               
- Phe Pro Ala Thr Val                                                     
    770                                                                   
- (2) INFORMATION FOR SEQ ID NO: 2:                                       
-      (i) SEQUENCE CHARACTERISTICS:                                      
#pairs    (A) LENGTH: 2322 base                                           
          (B) TYPE: nucleic acid                                          
          (C) STRANDEDNESS: single                                        
          (D) TOPOLOGY: linear                                            
-     (ix) FEATURE:                                                       
          (A) NAME/KEY: misc.sub.-- - #feature                            
          (B) LOCATION: (1-1269)                                          
#/note= &#34;FLP recombinase domain.&#34;                                         
-     (ix) FEATURE:                                                       
          (A) NAME/KEY: misc.sub.-- - #feature                            
          (B) LOCATION: (1270-1284)                                       
#/note= &#34;Linker peptide.&#34;MATION:                                          
-     (ix) FEATURE:                                                       
          (A) NAME/KEY: misc.sub.-- - #feature                            
          (B) LOCATION: (1285-2322)                                       
#/note= &#34;Estrogen binding domain.&#34;                                        
#2:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:                               
- ATG CCA CAA TTT GAT ATA TTA TGT AAA ACA CC - #A CCT AAG GTG CTT GTT     
  48                                                                      
Met Pro Gln Phe Asp Ile Leu Cys Lys Thr Pr - #o Pro Lys Val Leu Val       
#                 15                                                      
- CGT CAG TTT GTG GAA AGG TTT GAA AGA CCT TC - #A GGT GAG AAA ATA GCA     
  96                                                                      
Arg Gln Phe Val Glu Arg Phe Glu Arg Pro Se - #r Gly Glu Lys Ile Ala       
#             30                                                          
- TTA TGT GCT GCT GAA CTA ACC TAT TTA TGT TG - #G ATG ATT ACA CAT AAC     
 144                                                                      
Leu Cys Ala Ala Glu Leu Thr Tyr Leu Cys Tr - #p Met Ile Thr His Asn       
#         45                                                              
- GGA ACA GCA ATC AAG AGA GCC ACA TTC ATG AG - #C TAT AAT ACT ATC ATA     
 192                                                                      
Gly Thr Ala Ile Lys Arg Ala Thr Phe Met Se - #r Tyr Asn Thr Ile Ile       
#     60                                                                  
- AGC AAT TCG CTG AGT TCC GAT ATT GTC AAC AA - #G TCA CTG CAG TTT AAA     
 240                                                                      
Ser Asn Ser Leu Ser Ser Asp Ile Val Asn Ly - #s Ser Leu Gln Phe Lys       
# 80                                                                      
- TAC AAG ACG CAA AAA GCA ACA ATT CTG GAA GC - #C TCA TTA AAG AAA TTG     
 288                                                                      
Tyr Lys Thr Gln Lys Ala Thr Ile Leu Glu Al - #a Ser Leu Lys Lys Leu       
#                 95                                                      
- ATT CCT GCT TGG GAA TTT ACA ATT ATT CCT TA - #C TAT GGA CAA AAA CAT     
 336                                                                      
Ile Pro Ala Trp Glu Phe Thr Ile Ile Pro Ty - #r Tyr Gly Gln Lys His       
#           110                                                           
- CAA TCT GAT ATC ACT GAT ATT GTA AGT AGT TT - #G CAA TTA CAG TTC GAA     
 384                                                                      
Gln Ser Asp Ile Thr Asp Ile Val Ser Ser Le - #u Gln Leu Gln Phe Glu       
#       125                                                               
- TCA TCG GAA GAA GCA GAT AAG GGA AAT AGC CA - #C AGT AAA AAA ATG CTT     
 432                                                                      
Ser Ser Glu Glu Ala Asp Lys Gly Asn Ser Hi - #s Ser Lys Lys Met Leu       
#   140                                                                   
- AAA GCA CTT CTA AGT GAG GGT GAA AGC ATC TG - #G GAG ATC ACT GAG AAA     
 480                                                                      
Lys Ala Leu Leu Ser Glu Gly Glu Ser Ile Tr - #p Glu Ile Thr Glu Lys       
145                 1 - #50                 1 - #55                 1 -   
#60                                                                       
- ATA CTA AAT TCG TTT GAG TAT ACT TCG AGA TT - #T ACA AAA ACA AAA ACT     
 528                                                                      
Ile Leu Asn Ser Phe Glu Tyr Thr Ser Arg Ph - #e Thr Lys Thr Lys Thr       
#               175                                                       
- TTA TAC CAA TTC CTC TTC CTA GCT ACT TTC AT - #C AAT TGT GGA AGA TTC     
 576                                                                      
Leu Tyr Gln Phe Leu Phe Leu Ala Thr Phe Il - #e Asn Cys Gly Arg Phe       
#           190                                                           
- AGC GAT ATT AAG AAC GTT GAT CCG AAA TCA TT - #T AAA TTA GTC CAA AAT     
 624                                                                      
Ser Asp Ile Lys Asn Val Asp Pro Lys Ser Ph - #e Lys Leu Val Gln Asn       
#       205                                                               
- AAG TAT CTG GGA GTA ATA ATC CAG TGT TTA GT - #G ACA GAG ACA AAG ACA     
 672                                                                      
Lys Tyr Leu Gly Val Ile Ile Gln Cys Leu Va - #l Thr Glu Thr Lys Thr       
#   220                                                                   
- AGC GTT AGT AGG CAC ATA TAC TTC TTT AGC GC - #A AGG GGT AGG ATC GAT     
 720                                                                      
Ser Val Ser Arg His Ile Tyr Phe Phe Ser Al - #a Arg Gly Arg Ile Asp       
225                 2 - #30                 2 - #35                 2 -   
#40                                                                       
- CCA CTT GTA TAT TTG GAT GAA TTT TTG AGG AA - #T TCT GAA CCA GTC CTA     
 768                                                                      
Pro Leu Val Tyr Leu Asp Glu Phe Leu Arg As - #n Ser Glu Pro Val Leu       
#               255                                                       
- AAA CGA GTA AAT AGG ACC GGC AAT TCT TCA AG - #C AAC AAG CAG GAA TAC     
 816                                                                      
Lys Arg Val Asn Arg Thr Gly Asn Ser Ser Se - #r Asn Lys Gln Glu Tyr       
#           270                                                           
- CAA TTA TTA AAA GAT AAC TTA GTC AGA TCG TA - #C AAC AAA GCT TTG AAG     
 864                                                                      
Gln Leu Leu Lys Asp Asn Leu Val Arg Ser Ty - #r Asn Lys Ala Leu Lys       
#       285                                                               
- AAA AAT GCG CCT TAT TCA ATC TTT GCT ATA AA - #A AAT GGC CCA AAA TCT     
 912                                                                      
Lys Asn Ala Pro Tyr Ser Ile Phe Ala Ile Ly - #s Asn Gly Pro Lys Ser       
#   300                                                                   
- CAC ATT GGA AGA CAT TTG ATG ACC TCA TTT CT - #T TCA ATG AAG GGC CTA     
 960                                                                      
His Ile Gly Arg His Leu Met Thr Ser Phe Le - #u Ser Met Lys Gly Leu       
305                 3 - #10                 3 - #15                 3 -   
#20                                                                       
- ACG GAG TTG ACT AAT GTT GTG GGA AAT TGG AG - #C GAT AAG CGT GCT TCT     
1008                                                                      
Thr Glu Leu Thr Asn Val Val Gly Asn Trp Se - #r Asp Lys Arg Ala Ser       
#               335                                                       
- GCC GTG GCC AGG ACA ACG TAT ACT CAT CAG AT - #A ACA GCA ATA CCT GAT     
1056                                                                      
Ala Val Ala Arg Thr Thr Tyr Thr His Gln Il - #e Thr Ala Ile Pro Asp       
#           350                                                           
- CAC TAC TTC GCA CTA GTT TCT CGG TAC TAT GC - #A TAT GAT CCA ATA TCA     
1104                                                                      
His Tyr Phe Ala Leu Val Ser Arg Tyr Tyr Al - #a Tyr Asp Pro Ile Ser       
#       365                                                               
- AAG GAA ATG ATA GCA TTG AAG GAT GAG ACT AA - #T CCA ATT GAG GAG TGG     
1152                                                                      
Lys Glu Met Ile Ala Leu Lys Asp Glu Thr As - #n Pro Ile Glu Glu Trp       
#   380                                                                   
- CAG CAT ATA GAA CAG CTA AAG GGT AGT GCT GA - #A GGA AGC ATA CGA TAC     
1200                                                                      
Gln His Ile Glu Gln Leu Lys Gly Ser Ala Gl - #u Gly Ser Ile Arg Tyr       
385                 3 - #90                 3 - #95                 4 -   
#00                                                                       
- CCC GCA TGG AAT GGG ATA ATA TCA CAG GAG GT - #A CTA GAC TAC CTT TCA     
1248                                                                      
Pro Ala Trp Asn Gly Ile Ile Ser Gln Glu Va - #l Leu Asp Tyr Leu Ser       
#               415                                                       
- TCC TAC ATA AAT AGA CGC ATA TCC GTA CGC GG - #A TCC ATG AAA GGT GGG     
1296                                                                      
Ser Tyr Ile Asn Arg Arg Ile Ser Val Arg Gl - #y Ser Met Lys Gly Gly       
#           430                                                           
- ATA CGA AAA GAC CGA AGA GGA GGG AGA ATG TT - #G AAA CAC AAG CGC CAG     
1344                                                                      
Ile Arg Lys Asp Arg Arg Gly Gly Arg Met Le - #u Lys His Lys Arg Gln       
#       445                                                               
- AGA GAT GAT GGG GAG GGC AGG GGT GAA GTG GG - #G TCT GCT GGA GAC ATG     
1392                                                                      
Arg Asp Asp Gly Glu Gly Arg Gly Glu Val Gl - #y Ser Ala Gly Asp Met       
#   460                                                                   
- AGA GCT GCC AAC CTT TGG CCA AGC CCG CTC AT - #G ATC AAA CGC TCT AAG     
1440                                                                      
Arg Ala Ala Asn Leu Trp Pro Ser Pro Leu Me - #t Ile Lys Arg Ser Lys       
465                 4 - #70                 4 - #75                 4 -   
#80                                                                       
- AAG AAC AGC CTG GCC TTG TCC CTG ACG GCC GA - #C CAG ATG GTC AGT GCC     
1488                                                                      
Lys Asn Ser Leu Ala Leu Ser Leu Thr Ala As - #p Gln Met Val Ser Ala       
#               495                                                       
- TTG TTG GAT GCT GAG CCC CCC ATA CTC TAT TC - #C GAG TAT GAT CCT ACC     
1536                                                                      
Leu Leu Asp Ala Glu Pro Pro Ile Leu Tyr Se - #r Glu Tyr Asp Pro Thr       
#           510                                                           
- AGA CCC TTC AGT GAA GCT TCG ATG ATG GGC TT - #A CTG ACC AAC CTG GCA     
1584                                                                      
Arg Pro Phe Ser Glu Ala Ser Met Met Gly Le - #u Leu Thr Asn Leu Ala       
#       525                                                               
- GAC AGG GAG CTG GTT CAC ATG ATC AAC TGG GC - #G AAG AGG GTG CCA GGC     
1632                                                                      
Asp Arg Glu Leu Val His Met Ile Asn Trp Al - #a Lys Arg Val Pro Gly       
#   540                                                                   
- TTT GTG GAT TTG ACC CTC CAT GAT CAG GTC CA - #C CTT CTA GAA TGT GCC     
1680                                                                      
Phe Val Asp Leu Thr Leu His Asp Gln Val Hi - #s Leu Leu Glu Cys Ala       
545                 5 - #50                 5 - #55                 5 -   
#60                                                                       
- TGG CTA GAG ATC CTG ATG ATT GGT CTC GTC TG - #G CGC TCC ATG GAG CAC     
1728                                                                      
Trp Leu Glu Ile Leu Met Ile Gly Leu Val Tr - #p Arg Ser Met Glu His       
#               575                                                       
- CCA GTG AAG CTA CTG TTT GCT CCT AAC TTG CT - #C TTG GAC AGG AAC CAG     
1776                                                                      
Pro Val Lys Leu Leu Phe Ala Pro Asn Leu Le - #u Leu Asp Arg Asn Gln       
#           590                                                           
- GGA AAA TGT GTA GAG GGC ATG GTG GAG ATC TT - #C GAC ATG CTG CTG GCT     
1824                                                                      
Gly Lys Cys Val Glu Gly Met Val Glu Ile Ph - #e Asp Met Leu Leu Ala       
#       605                                                               
- ACA TCA TCT CGG TTC CGC ATG ATG AAT CTG CA - #G GGA GAG GAG TTT GTG     
1872                                                                      
Thr Ser Ser Arg Phe Arg Met Met Asn Leu Gl - #n Gly Glu Glu Phe Val       
#   620                                                                   
- TGC CTC AAA TCT ATT ATT TTG CTT AAT TCT GG - #A GTG TAC ACA TTT CTG     
1920                                                                      
Cys Leu Lys Ser Ile Ile Leu Leu Asn Ser Gl - #y Val Tyr Thr Phe Leu       
625                 6 - #30                 6 - #35                 6 -   
#40                                                                       
- TCC AGC ACC CTG AAG TCT CTG GAA GAG AAG GA - #C CAT ATC CAC CGA GTC     
1968                                                                      
Ser Ser Thr Leu Lys Ser Leu Glu Glu Lys As - #p His Ile His Arg Val       
#               655                                                       
- CTG GAC AAG ATC ACA GAC ACT TTG ATC CAC CT - #G ATG GCC AAG GCA GGC     
2016                                                                      
Leu Asp Lys Ile Thr Asp Thr Leu Ile His Le - #u Met Ala Lys Ala Gly       
#           670                                                           
- CTG ACC CTG CAG CAG CAG CAC CAG CGG CTG GC - #C CAG CTC CTC CTC ATC     
2064                                                                      
Leu Thr Leu Gln Gln Gln His Gln Arg Leu Al - #a Gln Leu Leu Leu Ile       
#       685                                                               
- CTC TCC CAC ATC AGG CAC ATG AGT AAC AAA GG - #C ATG GAG CAT CTG TAC     
2112                                                                      
Leu Ser His Ile Arg His Met Ser Asn Lys Gl - #y Met Glu His Leu Tyr       
#   700                                                                   
- AGC ATG AAG TGC AAG AAC GTG GTG CCC CTC TA - #T GAC CTG CTG CTG GAG     
2160                                                                      
Ser Met Lys Cys Lys Asn Val Val Pro Leu Ty - #r Asp Leu Leu Leu Glu       
705                 7 - #10                 7 - #15                 7 -   
#20                                                                       
- ATG CTG GAC GCC CAC CGC CTA CAT GCG CCC AC - #T AGC CGT GGA GGG GCA     
2208                                                                      
Met Leu Asp Ala His Arg Leu His Ala Pro Th - #r Ser Arg Gly Gly Ala       
#               735                                                       
- TCC GTG GAG GAG ACG GAC CAA AGC CAC TTG GC - #C ACT GCG GGC TCT ACT     
2256                                                                      
Ser Val Glu Glu Thr Asp Gln Ser His Leu Al - #a Thr Ala Gly Ser Thr       
#           750                                                           
- TCA TCG CAT TCC TTG CAA AAG TAT TAC ATC AC - #G GGG GAG GCA GAG GGT     
2304                                                                      
Ser Ser His Ser Leu Gln Lys Tyr Tyr Ile Th - #r Gly Glu Ala Glu Gly       
#       765                                                               
#2322              TC TGA                                                 
Phe Pro Ala Thr Val                                                       
    770                                                                   
__________________________________________________________________________