Patent Publication Number: US-2023141087-A1

Title: System for Viewing Cell Cultures Under a Microscope whilst Applying TTFields

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This Application is a divisional of U.S. application Ser. No. 15/872,318, filed Jan. 16, 2018, which claims the benefit of U.S. Provisional Application 62/448,152, filed Jan. 19, 2017, each of which is incorporated herein by reference in its entirety. 
    
    
     BACKGROUND 
     Tumor Treating Fields (TTFields) are low intensity electric fields in the intermediate frequency range that target solid tumors by disrupting mitosis. When treating patients, TTFields are delivered via transducer arrays made from a plurality of ceramic disks with a high dielectric constant (as described, for example, in U.S. Pat. No. 8,715,203, which is incorporated herein by reference). The transducer arrays capacitively couple the electric field into the patient&#39;s body. 
     The inovitro™ system supplied by Novocure is an existing system for studying TTFields in vitro. The inovitro™ system includes ceramic culture dishes constructed to capacitively couple electric field into the cell culture while the cell cultures are maintained at a controlled temperature. This is used to simulate the capacitive coupling of the electric fields into a patient&#39;s body. 
     SUMMARY OF THE INVENTION 
     One aspect of the invention is directed to a first apparatus for applying electric fields to a sample and for observing the sample using an inverted microscope while the sample is illuminated by a light source. The inverted microscope has a stage and an objective. This first apparatus comprises a bottom panel, ceramic sidewalls, a plurality of electrodes, and a plurality of electrical conductors. The bottom panel has a transparent region. The ceramic sidewalls are affixed to the bottom panel such that the ceramic sidewalls and the bottom panel cooperate to form a container for holding the sample, wherein the ceramic sidewalls are affixed to the bottom panel at a position that surrounds the transparent region, and wherein the ceramic sidewalls have at least one outer surface. The plurality of electrodes are disposed on the at least one outer surface of the ceramic sidewalls at positions selected so that when the sample is positioned in the container, application of a voltage between the plurality of electrodes induces an electric field through the sample. Each of the plurality of electrical conductors provides electrical contact with a respective one of the plurality of electrodes. The bottom panel, the transparent region, and the ceramic sidewalls are sized and shaped to facilitate positioning of the container on the stage of the inverted microscope so that when the sample is positioned in the container, light emanating from the light source is free to travel along an optical path that passes through the sample, through the transparent region, and into the objective of the inverted microscope. And each of the plurality of electrodes and each of the plurality of electrical conductors is positioned with respect to the transparent region so as not to interfere with the optical path. 
     In some embodiments of the first apparatus, the ceramic sidewalls are cylindrical. 
     Some embodiments of the first apparatus further comprise at least one thermistor mounted to the ceramic sidewalls at a position that does not interfere with the optical path. Some embodiments of the first apparatus further comprise at least one thermistor mounted to the bottom panel at a position that does not interfere with the optical path. 
     In some embodiments of the first apparatus, the ceramic sidewalls are mounted to the bottom panel using an adhesive. 
     In some embodiments of the first apparatus, the ceramic sidewalls are mounted to the bottom panel using a screw mount configured to squeeze the ceramic sidewalls and the bottom panel together. Some of these embodiments further comprise an O-ring disposed between the ceramic sidewalls and the bottom panel. 
     In some embodiments of the first apparatus, the ceramic sidewalls are mounted to the bottom panel using an O-ring that has (a) an outer diameter that matches the inner diameter of a Petri dish and (b) an inner diameter that matches the outer diameter of the cylindrical sidewalls, and the cylindrical sidewalls are jammed into the O-ring to provide an interference fit. 
     In some embodiments of the first apparatus, the entire bottom panel is transparent. 
     Some embodiments of the first apparatus further comprise at least one thermistor mounted to the container at a position that does not interfere with the optical path. In these embodiments, the entire bottom panel is transparent, the ceramic sidewalls are cylindrical, and the second direction is roughly perpendicular to the first direction. 
     In some embodiments of the first apparatus, the plurality of electrodes comprises at least four electrodes disposed on the at least one outer surface of the ceramic sidewalls at positions selected so that when the sample is positioned in the container, (a) application of a voltage between a first subset of the at least four electrodes induces an electric field in a first direction through the sample, and (b) application of a voltage between a second subset of the at least four electrodes induces an electric field in a second direction through the sample. The plurality of electrical conductors comprises at least four electrical conductors, and each of the at least four electrical conductors provides electrical contact with a respective one of the at least four electrodes. In some of these embodiments, the second direction is roughly perpendicular to the first direction. 
     Another aspect of the invention is directed to a second apparatus for optically observing a sample while applying electric fields to the sample. This second apparatus comprises a fluid tight container; first, second, third, and fourth electrodes; and first, second, third, and fourth electrical conductors. The fluid-tight container is shaped and dimensioned for holding the sample, and the container has a transparent bottom panel and ceramic sidewalls affixed to the transparent bottom panel, wherein the ceramic sidewalls have at least one outer surface. The first electrode is disposed at a first position on the at least one outer surface of the ceramic sidewalls. The second electrode is disposed at a second position on the at least one outer surface of the ceramic sidewalls, wherein the second position is opposite to the first position, so that application of an AC voltage between the first electrode and the second electrode induces an AC electric field through the sample in a first direction. The third electrode is disposed at a third position on the at least one outer surface of the ceramic sidewalls. The fourth electrode is disposed at a fourth position on the at least one outer surface of the ceramic sidewalls, wherein the fourth position is opposite to the third position, so that application of an AC voltage between the third electrode and the fourth electrode induces an AC electric field through the sample in a second direction. The first electrical conductor is arranged to route electricity between a first electrical terminal and the first electrode along a path that circumvents the transparent bottom panel. The second electrical conductor is arranged to route electricity between a second electrical terminal and the second electrode along a path that circumvents the transparent bottom panel. The third electrical conductor is arranged to route electricity between a third electrical terminal and the third electrode along a path that circumvents the transparent bottom panel. And the fourth electrical conductor is arranged to route electricity between a fourth electrical terminal and the fourth electrode along a path that circumvents the transparent bottom panel. 
     In some embodiments of the second apparatus, the first electrical terminal, the second electrical terminal, the third electrical terminal, and the fourth electrical terminal are all disposed in a single electrical connector. In some embodiments of the second apparatus, the ceramic sidewalls are cylindrical. 
     Some embodiments of the second apparatus further comprise at least one thermistor mounted to the ceramic sidewalls. 
     In some embodiments of the second apparatus, the ceramic sidewalls are mounted to the bottom panel using an adhesive. In some of these embodiments, the container has an O-ring disposed between the ceramic sidewalls and the bottom panel, and the ceramic sidewalls are mounted to the bottom panel using a screw mount configured to squeeze the ceramic sidewalls and the bottom panel together. 
     In some embodiments of the second apparatus, the ceramic sidewalls are mounted to the bottom panel using an O-ring that has (a) an outer diameter that matches the inner diameter of a Petri dish and (b) an inner diameter that matches the outer diameter of the cylindrical sidewalls, and the cylindrical sidewalls are jammed into the O-ring to provide an interference fit. 
     In some embodiments of the second apparatus, the second direction is roughly perpendicular to the first direction. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG.  1    is an assembled view of a first embodiment of an apparatus for viewing cell cultures under a microscope whilst applying TTFields. 
         FIG.  2    is an exploded view of the  FIG.  1    apparatus. 
         FIG.  3    depicts the  FIG.  2    view with the upper housing removed in order to reveal the ceramic sidewalls. 
         FIG.  4    depicts a perspective view of the cylindrical ceramic sidewalls of the  FIG.  1    embodiment. 
         FIG.  5    depicts a schematic plan of view of the components that appear in  FIG.  4   . 
     
    
    
     Various embodiments are described in detail below with reference to the accompanying drawings, wherein like reference numerals represent like elements. 
     DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     One limitation of the inovitro™ system is that the construction of the ceramic culture dishes and the circuitry for applying electrical signals to the electrodes on the dishes makes it impossible to perform live-cell microscopy experiments on cells while those cells are being subjected to TTFields. The embodiments described below overcome this disadvantage. 
       FIGS.  1 - 5    depict various views of an embodiment of a dish-like apparatus for applying electric fields to a sample and for observing the sample using an inverted microscope while the sample is illuminated by a light source. This embodiment facilitates time lapse microscopy during the application of TTFields. The inverted microscope that is used to observe the sample will typically have a stage and an objective. The dish-like apparatus may be designed to sit directly on the stage of the inverted microscope while the inverted microscope is positioned in an incubator (which optionally may provide any necessary gases to the sample). 
     To allow light to flow through the sample for the purpose of microscopy experiments or other imaging techniques, the dish-like apparatus has a bottom panel  20  with a transparent region. In the illustrated embodiment, the entire bottom panel  20  is transparent. However, in alternative embodiments, only a portion of the bottom panel  20  may be transparent. In the illustrated embodiment, the floor of a glass Petri dish  10  serves as the bottom panel  20 . Preferably, the transparent region does not introduce optical distortions. In those embodiments that use the floor of a glass Petri dish to serve as the bottom panel, ibidi® dishes may be used to minimize optical distortions. 
     Ceramic sidewalls  30  (visible in  FIGS.  3 - 5   ) are affixed to the bottom panel  20  such that the ceramic sidewalls  30  and the bottom panel  20  cooperate to form a container that is shaped and dimensioned for holding the sample. In those embodiments where only a portion of the bottom panel  20  is transparent, the ceramic sidewalls  30  are affixed to the bottom panel  20  at a position that surrounds the transparent region. In those embodiments where the entire bottom panel  20  is transparent, the ceramic sidewalls  30  may be affixed to the bottom panel  20  anywhere (e.g. close to the perimeter of the bottom panel). In this case, a portion of the bottom panel  20  that lies within the boundaries of the ceramic sidewalls  30  will serve as the transparent region. The ceramic sidewalls  30  are preferably made from a of a high capacitance material (e.g. PMN-PT). 
     The bottom panel  20 , the transparent region of the bottom panel  20 , and the ceramic sidewalls  30  are sized and shaped to facilitate positioning of the container on the stage of the inverted microscope so that when the sample is positioned in the container, light emanating from the light source is free to travel along an optical path that passes through the sample, through the transparent region of the bottom panel  20 , and into the objective of the inverted microscope. 
     In some preferred embodiments (including the illustrated embodiment), the ceramic sidewalls  30  are formed from a single cylindrical tube. In these embodiments, the ceramic sidewalls  30  will have a single cylindrical outer surface. In alternative embodiments, ceramic sidewalls  30  with different shapes may be used (e.g. square or octagonal). In these embodiments, the ceramic sidewalls  30  will have two or more outer surfaces. For example, in those embodiments where the square ceramic sidewalls are used, the ceramic sidewalls will have four outer surfaces. 
     In some embodiments, the ceramic sidewalls  30  are mounted to the bottom panel  20  using an adhesive (e.g., biocompatible glue or cement). 
     In alternative embodiments, the ceramic sidewalls  30  are mounted to the bottom panel  20  using a screw mount configured to squeeze the ceramic sidewalls  30  and the bottom panel  20  together. For example, the upper housing  70  can connect to a Petri dish  10  using a threaded screw-mount connection which includes a set of external threads (not shown) on the upper housing  70  and a corresponding set of internal threads (not shown) on the Petri dish  10 . In these embodiments, an O-ring  75  is preferably positioned between the ceramic sidewalls  30  and the bottom panel  20  of the Petri dish  10  such that the O-ring  75  is compressed when the upper housing  70  is screwed into the Petri dish  10 . The O-ring  75  seals liquids into the volume defined by the bottom panel  20  and the cylindrical sidewalls  30 . The upper housing  70  has an opening  72  through which samples can be inserted into the Petri dish  10 . 
     In alternative embodiments, instead of using a screw mount, an O-ring  75  that has (a) an outer diameter that matches the inner diameter of the Petri dish  10  and (b) an inner diameter that matches the outer diameter of the cylindrical sidewalls  30  is used, and the cylindrical sidewalls  30  are jammed into the O-ring  75  to provide an interference fit. The 35 mm m-dish made by Ibidi GmbH (ibid.com) is suitable for use as the Petri dish  10  in this embodiment. 
     Note that in those embodiments that use the floor of a glass Petri dish  10  to serve as the bottom panel  20 , the vertical walls of the Petri dish  10  are disposed radially beyond the ceramic sidewalls  30 . 
     The height of the ceramic sidewalls  30  may be varied to allow different amounts of media to be placed within each container as well as to accommodate possible inserts (e.g. Boyden inserts). In alternative embodiments, tall containers may be obtained by grafting a second cylinder made of a biocompatible material (e.g., glass or polycarbonate) to the top of a short ceramic cylinder. Positioning the ceramic cylinder at the bottom of the container facilitates the application of TTFields to bottom of the container where cells are plated. 
     Optionally, tubing  90  is provided to allow for media replacement without the need to remove the dish  10  from the stage of the microscope (not shown) while maintaining sterile conditions. Although only a single piece of tubing  90  is depicted in  FIGS.  1 - 3   , two or more pieces of tubing (not shown) may be used in alternative embodiments. 
     As best seen in  FIGS.  4 - 5   , a plurality of electrodes  41 - 44  is disposed on the at least one outer surface of the ceramic sidewalls  30  at positions selected so that when the sample is positioned in the container, application of a voltage between the plurality of electrodes  41 - 44  induces an electric field through the sample. In some embodiments, at least four electrodes  41 - 44  are disposed on the outer surface (or surfaces) of the ceramic sidewalls  30  at positions selected so that when the sample is positioned in the container, (a) application of a voltage between a first subset of the at least four electrodes induces an electric field in a first direction through the sample, and (b) application of a voltage between a second subset of the at least four electrodes induces an electric field in a second direction through the sample. The electrodes  41 - 44  may be formed on the outer surface of the ceramic sidewalls  30  by painting panels of a conductive material directly onto the outer surface, by applying a thin sheet of a conductive material using a suitable conductive adhesive, by compressing a thin leaf of metal (e.g., gold) directly onto the outer surface of the ceramic sidewalls  30 , or by a variety of alternative approaches that will be apparent to persons skilled in the relevant arts. 
     The electrodes  41 - 44  and the region of the ceramic sidewalls  30  beneath the electrodes  41 - 44  form capacitive electrodes through which the electric field is coupled into the sample (i.e. the cell culture). The advantage of using a ceramic with a high relative permittivity is that the impedance of the electrodes can be kept low whilst maintaining the walls at a thickness that ensures the mechanical rigidity of the dish-like apparatus. 
     In the illustrated embodiment and as best seen in  FIG.  5   , the sidewalls  30  are cylindrical and four electrodes  41 - 44  are positioned on respective quadrants of the cylindrical sidewalls  30 . The first electrode  41  is disposed at a first position on the cylindrical sidewalls  30  and the second electrode  42  is disposed at a second position on the cylindrical sidewalls  30  that is opposite to the first position. The third electrode  43  is disposed at a third position on the cylindrical sidewalls  30  and the fourth electrode  44  is disposed at a fourth position on the cylindrical sidewalls  30  that is opposite to the third position. 
     Application of an AC voltage between a first subset of electrodes consisting of electrodes  41  and  42  induces an electric field in a first direction through the sample. Application of an AC voltage between a second subset of electrodes consisting of electrodes  43  and  44  induces an electric field in a second direction through the sample. When the electrodes are arranged as depicted in  FIGS.  4 - 5   , the second direction is perpendicular to the first direction. If one subset of electrodes (e.g. electrodes  41  and  42 ) is shifted by a small angle (e.g. less than 10°), the second direction will be roughly perpendicular to the first direction. 
     A plurality of electrical conductors  50  is provided, and each of the plurality of electrical conductors  50  provides electrical contact with a respective one of the plurality of electrodes  41 - 44  and routes electricity between a given one of those electrodes  41 - 44  and a respective corresponding electrical terminal. In embodiments that have at least four electrodes, at least four electrical conductors  50  are provided, and each of the at least four electrical conductors  50  provides electrical contact between a respective one of at least four electrical terminals and a respective one of the at least four electrodes  41 - 44 . The conductors  50  may be implemented using individual wires, ribbon cables, flex circuits, etc. Each of the conductors  50  may be connected to the electrodes  41 - 44  using any appropriate approach including but not limited to soldering, electrical connectors, etc. In some embodiments, each of the electrical terminals is disposed in a single electrical connector  52  (shown in  FIGS.  2  and  3   ). 
     Each of the plurality of electrodes  41 - 44  and each of the plurality of electrical conductors  50  is positioned with respect to the transparent region of the bottom panel  20  so as not to interfere with the optical path described above. For example, in those embodiments where the entire bottom panel  20  is transparent, each of the plurality of electrical conductors  50  may traverse a path that circumvents the entire transparent bottom panel  20 . The conductors are used for applying electric fields to a sample that is positioned in the container. For example, an AC voltage between 50 and 500 kHz may be applied across the conductors that are wired to the first electrode pair  41 - 42  and then across the conductors that are wired to the second electrode pair  43 - 44  in an alternating and repeating sequence. This will cause electric fields with different directions to be generated in the samples that are located in the container in a corresponding alternating and repeating sequence. In alternative embodiments, the voltages may be applied across different combinations of the electrodes  41 - 44  in a different sequence to provide alternative field shapes or directions. 
     The dish-like apparatus described herein is useful for various assays such as: watching the evolution of cellular structures in response to TTFields; using fluorescent dyes, GFP-tagged proteins, or other labeled proteins; scanning frequencies to determine the most effective frequency; measuring cells&#39; sensitivity assays to different TTFields intensities; measuring the diameter of cells; measuring migration rates and directions during TTFields application; determining TTFields&#39; effect on cell invasion using a Boyden chamber inserted into the container; determining TTFields&#39; effect intracellular on different structures/molecules within the cell; and determining TTFields&#39; effect on cell grown in 3D structure (e.g. microspheres) using specific inserts which maintain and support the 3D structures (e.g. agarose mesh). 
     As best seen in  FIGS.  2 - 3   , in some embodiments, the container has a transparent cover  80  that maintains sterile conditions within the container and minimizes evaporation while allowing for gas exchange. The cover  80  is preferably made of a transparent material that allows light from the microscope condenser to reach sample (i.e., the cell culture), thereby enabling imaging of the sample using the light from the condenser. Optionally, a heating element may be provided to heat the transparent cover  80  to prevent liquid condensation from interfering with the viewing of the sample. Optionally, temperature sensors may be used to control the temperature of the cover  80 . 
     Turning now to  FIG.  3   , at least one temperature sensor (e.g., thermistor  60 ) is preferably provided in thermal contact with the container (which, as described above, is formed from the ceramic sidewalls  30  and the bottom panel  20 ) to measure the temperature of the container. The thermistor or thermistors  60  are mounted at a position that does not interfere with the optical path described above. In some embodiments, the thermistor or thermistors  60  are mounted to the ceramic sidewalls  30  at a position that does not interfere with the optical path. In alternative embodiments, the thermistor or thermistors  60  are mounted to the bottom panel  20  at a position that does not interfere with the optical path. In some embodiments, the at least one thermistor  60  comprises two thermistors that are mounted on opposite sides of the ceramic sidewalls  30 . The thermistors may be connected to ceramic sidewalls  30  using a heat conductive adhesive. The electrical wiring to the thermistors  60  (not shown) should not interfere with the optical path. In alternative embodiments, different types of temperature sensors may be used in place of thermistors. 
     When applying TTFields to cell cultures, Ohmic losses in the cell culture heat the cell culture medium. The thermistor or thermistors  60  are used to monitor the temperature in the container. The electric field intensity and/or the ambient temperature can then be controlled to maintain the desired temperature in the cell culture. 
     One example of a temperature control algorithm that is suitable for use when applying TTFields is provided below. The data from the thermistors attached to the ceramic sidewalls  30  is transferred to a processor (not shown). The processor compares the current temperature of the hottest thermistor with the temperature recorded in the previous 20 measurements and provides a prediction regarding the temperature to be reached within the next 20 measurements in the current settings. The predication is based on the following equations: 
         D =[( T   (n)   −T   (n−20) )]  Equation 1
 
         T   (n+20)   =T   n   +D   Equation 2
 
     Where:
         D—temperature change in the last 20 measurements   T n —Last temperature measured   T (n+20) —Predicted temperature within the next 20 measurements       

     Based upon the predicted temperature the algorithm determines the change in the electric fields intensity by changing the output currents, thus allowing for the temperature within the dish-like apparatus to reach the target temperature T TARGET  with minimal overshooting. The degree of change in the output current is based upon the following scheme: 
     1. If (T TARGET −0.4)&lt;T (n+20) &lt;T TARGET →don&#39;t change current 
     2. If T n &lt;(T TARGET −0.4) and D&gt;0.3→don&#39;t change current 
     3. If T n &lt;(T TARGET −0.4) and D*20&gt;0.8→don&#39;t change current 
     4. If 1-3 are false, change current as follows:
         If cooling if needed→Reduce the current by (T (n+20) −T TARGET )* K 1 , but not more than K 2 .   If heating is needed→Increase the current by (T TARGET −T (n+20) )* K 3 , but not more than K 4 .       

     Where: 
     T TARGET  is the target temperature 
     K 1 —a constant describing the current step down 
     K 2 —a constant describing the current maximal step down 
     K 3 —a constant describing the current step up 
     K 4 —a constant describing the current maximal step up 
     This algorithm takes into account the differences between the temperature of the ceramic sidewalls  30  and the ambient temperature and provides accurate estimation of the temperature within the container (e.g., ±1° C.). The precise estimation of the temperature within the container is based upon extensive temperature measurements performed using the thermistors attached to the ceramic sidewalls  30 . Optionally, temperature probes inserted into the media within the container may be used to supplement the temperature measurements obtained using the thermistors. 
     The algorithm also ensures that the temperature increase within the container from room temperature to the target temperature will take at least 25 minutes to follow the temperature rate increase in control dishes when transferred from room temperature to an incubator set to the target temperature. 
     In some preferred embodiments, temperature measurements are made every 1-5 seconds (e.g., every 3 seconds); the prediction algorithm starts every 2-10 seconds (e.g., every 6 seconds), and the values of the constants K 1 -K 4  are as follows: 
     K 1  is between 1 and 5 mA (e.g., 2 mA or 2.2 mA) 
     K 2 =4×K 1    
     K 3 =K 1    
     K 4 =2×K 3    
     In some preferred embodiments the maximum measured load current is 546 mA; the maximum measured output voltage is 210 V; and the algorithm works with the digital trimmer steps, with a minimal current of 25 steps (e.g., corresponding to 54.6 mA) and a maximum current of 250 steps (e.g., corresponding to 546 mA). 
     While the present invention has been disclosed with reference to certain embodiments, numerous modifications, alterations, and changes to the described embodiments are possible without departing from the sphere and scope of the present invention, as defined in the appended claims. Accordingly, it is intended that the present invention not be limited to the described embodiments, but that it has the full scope defined by the language of the following claims, and equivalents thereof.