Patent Publication Number: US-2011072636-A1

Title: Method of assembling a porous membrane cartridge

Description:
This is a divisional of application Ser. No. 10/575,366 filed Apr. 11, 2006 and PCT/JP04/014156, filed Sep. 28, 2004. The entire disclosure of the prior applications, application Ser. No. 10/575,366 and PCT/JP04/014156 are considered part of the disclosure of the accompanying divisional application and are hereby incorporated by reference. 
    
    
     TECHNICAL FIELD 
     The present invention relates to a porous membrane cartridge used for a filtration of a liquid and the like. 
     BACKGROUND ART 
     A porous membrane is widely used in a laboratory and a factory for a filtration of a liquid and an adsorption of a specific substance in the liquid. Then in using the porous membrane for such a purpose, it is requested to hold the porous membrane on the way of a passage where the liquid passes. As the holding method is generally used a method of holding the porous membrane with sandwiching it between two members having a passage where the liquid passes. 
     Because such a porous membrane is generally used in a precise experiment and measurement, a purified one is requested and is usually changed if once used. Therefore, it is convenient in points of a purification and an expedience in use to make a porous membrane a cartridge holding the porous membrane in a state of being able to pass a liquid therethrough. As such a porous membrane is known, for example, such a nucleic acid refining unit described in Japanese Patent Laid-Open Publication No. 2002-345465. 
     DISCLOSURE OF THE INVENTION 
     However, in a conventional porous membrane there is a fear that a liquid to pass the porous membrane goes around a side thereof. Particularly, in a case that two components for sandwiching the porous membrane are fixed only by fastening with fit-in, a size changes according to an expansion by a temperature change and swelling by a humidity change, and a sandwiched state changes in some case. In addition, in a case of using the conventional porous membrane in refining a nucleic acid, although the nucleic acid is adsorbed to the porous membrane, then is adsorbed to the porous membrane, and making a specific liquid flow, the nucleic acid is desorbed; however, if unable to make the liquid for desorption sufficiently flow also in corner portions of the porous membrane, there is a problem that: the nucleic acid remains in the corners and a recovery efficiency of the nucleic acid worsens. In view of such a problem, the present invention is made, and a purpose thereof is to provide a porous membrane cartridge for preventing a liquid from going around a side portion thereof. 
     In order to solve the problem, a porous membrane cartridge of the present invention is configured as follows: Namely, the porous membrane cartridge comprises a cylindrical barrel having openings at a top end and a rear end, respectively; a cap formed into a cylindrical shape having a fit-in portion fitted outside the top end, abutting with an opening edge of the top end, and having a sandwiching face for sandwiching a porous membrane between itself and the barrel; and the porous membrane sandwiched between the opening edge of the barrel and the cap, wherein the cap is fixed to the barrel so as not to be pulled out of the barrel in a state of crushing a peripheral edge of the porous membrane and sandwiching the porous membrane between itself and the cap. 
     In accordance with such a porous membrane cartridge, an opening edge of a barrel and a sandwiching face of a cap are sandwiched in a state of a peripheral edge of the porous membrane being crushed. In other words, if because the porous membrane has a porosity, it is strongly sandwiched by the sandwiching face between the opening edge of the barrel and the sandwiching face of the cap, the porosity is crushed as if occluded, and a liquid results in not flowing therefrom. Then because in the porous membrane cartridge the cap is fixed to the barrel in this state, the liquid does not go around a side portion of the porous membrane. Accordingly, when using the porous membrane cartridge in filtration and adsorption, it is enabled to prevent a discharged liquid from being contaminated by a liquid going around the side portion of the porous membrane. 
     As a method of fixing the cap to the barrel can be used any one of a fit-in, adhesion, ultrasonic welding, laser welding, insert molding, and the like. To be more precise, it is preferable that the opening edge of the barrel and the sandwiching face of the cap are welded by ultrasound. 
     Then, in addition that the opening edge of the barrel is formed to a taper where an inner perimeter side is more retreated than an outer perimeter side, it is preferable that the opening edge of the barrel is welded to the sandwiching face of the cap by ultrasound. Thus because in addition to crushing a peripheral edge of the porous membrane by a portion of an outer perimeter side, welding can be performed by the portion of the outer perimeter and a sandwiching force of the porous membrane gradually becomes smaller as the porous membrane draws apart from the welded portion (namely, the portion of the outer perimeter), it is enabled to avoid a stress on the porous membrane from concentrating and to prevent a breakage thereof from occurring. At this time, because if the taper is continuously formed into a flat portion formed at an outmost perimeter of the opening edge, it sandwiches the porous membrane by a clear welded portion (energy director), it is enabled to reduce an unnecessary positional displacement of the porous membrane. 
     In this case, in addition to sandwiching and crushing the porous membrane between the outer perimeter side of the opening edge and the sandwiching face, it is preferable that a portion of the outer perimeter side and that of the sandwiching face are welded therebetween by ultrasound as it is. 
     In addition, a porous membrane cartridge may also be configured by: circularly forming a bead portion as a welded portion (energy director), additionally the porous membrane is sandwiched and crushed with the bead portion, and the cap and the barrel are welded by ultrasound. 
     In addition, any one of the adhesion portion and welded portion of the barrel and the cap is not limited to the opening edge and the sandwiching face. For example, the barrel may also have a joint portion for abutting with an opening edge of a fit-in portion of the cap on own outer perimeter face, make the cap and the barrel adhere or be welded between the opening edge and the joint portion, and thereby configure a porous membrane cartridge. Thus, if sandwiching a porous membrane and jointing a barrel and a cap at separate portions, it does not occur to damage the porous membrane even in a case of using an especially delicate porous membrane. 
     Furthermore, in a case of fixing the cap by fitting in the barrel, it is enabled to fix the cap to the barrel by an engagement between any one of a depression and a protrusion formed on an outer perimeter face of the barrel and any one of those formed on an inner perimeter face of the cap. At this time, not to mention an engagement between the depression on the outer perimeter face of the barrel and the protrusion on the inner perimeter face of the cap and an engagement between the protrusion on the outer perimeter face of the barrel and the depression on the inner perimeter face of the cap, the protrusion on the outer perimeter face of the barrel and the protrusion on the inner perimeter face of the cap may be engaged. 
     In addition, not limiting to the portions described above, it is also enabled to fix the cap and the barrel by any one of an adhesive and laser welding on a fit-in face where the outer perimeter face of the barrel and the inner perimeter face of the cap oppose each other. 
     In addition, in a case of performing molding by insert molding, it is preferable to place a cap molded in advance and a porous membrane disposed in the cap within a cavity of an injection molding mold, to inject a molding material in the cavity, to thereby mold the barrel, and to simultaneously sandwich a porous membrane with the opening edge of the barrel and the sandwiching face of the cap. Thus it is enabled to realize a sandwich and fixation of the porous membrane. In addition, in a case of making a porous membrane cartridge unit where a plurality of porous membrane cartridges are connected, it is enabled to align a sandwiching force of porous membranes of the plurality of the porous membrane cartridges, and while simultaneously forming a plurality of barrels, to make up a state of these barrels being stably connected. 
     In accordance with a porous membrane cartridge of the present invention thus described, it is enabled to prevent a liquid from going around a side portion of the porous membrane. 
    
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         FIG. 1  is an exploded perspective view of a porous membrane cartridge related to a first embodiment of the present invention. 
         FIG. 2  is a section view of a porous membrane cartridge related to the first embodiment. 
         FIG. 3  is an enlarged section perspective view of a cap related to the first embodiment. 
         FIG. 4  is an enlarged view of a part A in  FIG. 2 , and shows a state before a barrel  10  and a cap  20  are welded. 
         FIG. 5  is drawings showing a process of assembling a porous membrane cartridge related to the first embodiment; (a) shows a state before welding; (b) shows a state of starting to crush a porous membrane F; (c) shows a state on the way of the welding; and (d) shows a state after the welding. 
         FIG. 6  is section views showing a porous membrane cartridge  1 A related to a second embodiment of the present invention and corresponding to the enlarged view of the part A in  FIG. 2 ; (a) shows a state before welding; (b) shows a state on the way of the welding; and (c) shows a state after the welding. 
         FIG. 7  is a section view showing another example of the porous membrane cartridge  1 A related to the second embodiment and corresponding to the enlarged view of the part A in  FIG. 2 . 
         FIG. 8  is drawings showing a porous membrane cartridge  1 D related to a third embodiment of the present invention and corresponding to the enlarged view of the part A in  FIG. 2 ; (a) shows a state before welding; and (b) shows a state after the welding. 
         FIG. 9(   a ) is another example of the porous membrane cartridge  1 D of the third embodiment; and  FIG. 9(   b ) shows still another example. 
     
    
    
     BEST MODE(S) FOR CARRYING OUT THE INVENTION 
     First Embodiment 
     Next will be described an embodiment of the present invention, referring to drawings as needed.  FIG. 1  is an exploded perspective view of a porous membrane cartridge related to a first embodiment of the present invention;  FIG. 2  is a section view of a porous membrane cartridge related to the first embodiment; and  FIG. 3  is an enlarged section perspective view of a cap related to the first embodiment. 
     Meanwhile, although in the embodiment is described a case of using a porous membrane cartridge in a nucleic acid as an application thereof, the application is not limited thereto. 
     As shown in  FIG. 1 , a porous membrane cartridge  1  related to the first embodiment is configured of a porous membrane F, and a barrel  10  and a cap  20  for holding the porous membrane F and forming a passage where a liquid passes. 
     The barrel  10  comprises a cylindrical main body portion  12  and a cylindrical top end  13  continuing into the main body portion  12 , and has an opening  11   a  at the top end  13  and an opening  11   b  at a rear end of the main body portion  12 . Therefore, a liquid is enabled to pass from the opening  11   b  to the opening  11   a . An outer diameter of the top end  13  is designed to be one size smaller that of the main body portion  12 . Meanwhile, in the embodiment a side of the barrel  10  and the cap  20  in which a liquid flows is called a rear end, and a side from which the liquid is pushed out is called a top end. 
     The cap  20  comprises a cylindrical fit-in portion  22  and a nozzle  23  continuing into a top side of the fit-in portion  22 . 
     An opening  21   a  is formed at the top end of the nozzle  23 , an opening  21   b  is formed at the rear end of the fit-in portion  22 , and thus a liquid is enabled to pass from the rear end to top end of the cap  20 . 
     An inner diameter of the fit-in portion  22  is formed into a diameter enabled to fit in the diameter of the top end  13  of the barrel  10 . 
     Then as shown in  FIG. 2 , by fitting the top end  13  of the barrel  10  in the fit-in portion  22  of the cap  20  in a state of putting in the porous membrane F into the portion  22  of the cap  20 , it is enabled to sandwich the porous membrane F between the cap  20  and the barrel  10 . 
     As shown in  FIG. 3 , in the cap  20  are formed six radial ribs  25  (only three are shown in  FIG. 3 ) at a bottom portion  26  of the fit-in portion  22  continuing into the nozzle  23  from the portion  22 . In addition, at an outer perimeter edge of the bottom portion  26 , across a whole perimeter thereof is formed a sandwiching face  24  made higher by one step from the bottom portion  26  so as to be a same height as an upper face of the ribs  25 . 
     The sandwiching face  24  is a face for sandwiching the porous membrane F between itself and an opening edge  14  (see  FIG. 1 ) corresponding to an end edge of the opening  11   a  of the barrel  10 . 
     The ribs  25  are formed at the same height as the sandwiching face  24 , thereby support the porous membrane F disposed at the bottom portion  26  within the cap  20 , and prevent the porous membrane F from elongating and breaking by a liquid flowing into the top end (opening  21   a ) from the rear end (opening  21   b ). In addition, the ribs  25  are radially formed, and thereby, it is designed that a liquid smoothly flows in the nozzle  23  in being made to flow from the rear end to the top end. 
     Meanwhile, although the barrel  10  and the cap  20  are composed of polystyrene, they are not limited thereto. In a case of fixing the barrel  10  and the cap  20  by ultrasonic welding, a thermoplastic resin is available that can be welded by ultrasound. In addition, in a case of fixing the barrel  10  and the cap  20  by adhesive, a material is available that can be made to adhere by adhesive. 
     The porous membrane F is composed of an organic polymer and is formed into a circle of which a diameter approximately matches the inner diameter of the cap  20  and the outer diameter of the top end  13  of the barrel  10 . As a material of the porous membrane F, for example, a surface-saponified substance of acetylcellulose is suitable. As acetylcellulose, although any one of mono acetylcellulose, diacetylcellulose, and triacetylcellulose is available, specifically triacetylcellulose is preferable. 
     Meanwhile, as a general filter can also be used a porous membrane of such PTFE (polytetrafluoroethylene), polyamide, polypropylene, and polycarbonate. 
       FIG. 4  is an enlarged view of a part A in  FIG. 2 . 
     As shown in  FIG. 4 , in the opening edge  14  of the barrel  10 , a slight portion (for example, a degree of 0.1 mm width) from an outmost perimeter is formed as a flat portion  14   a  perpendicular to an axial line (longitudinal direction) of the barrel  10 , and continuing into the flat portion  14   a  is formed a taper portion  14   b  that becomes an inside taper that gradually retreats from an outer perimeter toward an inner perimeter thereof. The taper portion  14   b  makes a small angle of 3 to 20 degrees, for example, 10 degrees with the flat portion  14   a . A diameter of an end  14   c  at an inner perimeter side of the taper portion  14   b  approximately matches that of an end  24   a  at an inner perimeter side of the sandwiching face  24 , and from the end  14   c  across the inner perimeter face  13   a  of the barrel  10 , a chamfer  15  is formed that makes an angle of 60 degrees with the flat portion  14   a.    
     In addition, it is preferable to set a longitudinal direction distance of the barrel  10  that retreats from the flat portion  14   a  to the end  14   c  so as to be a same degree as a thickness of the porous membrane F and so that the end  14   c  abuts with a surface of the membrane F after welding the barrel  10  and the cap  20 . 
     Although such an outer diameter of the opening edge  14  of the barrel  10  is decided according to a process liquid amount and a filtration time, in a case of fixing the barrel  10  and the cap  20  by ultrasonic welding, it is preferable to press the flat portion  14   a  to the porous membrane F and vibrate the portion  14   a , and to thereby set a width of the portion  14   a  to a size suitable for the membrane F melting. For example, in a case that the outer diameter of the opening edge  14  is 7 mm, the width of the flat portion  14   a  is preferably 0.02 to 1 mm. This is because a filtration effective area decreases if the width of the flat portion  14   a  is made too wide. Furthermore, considering a manufacturing adequacy such as an energy added to the barrel  10  in welding, the width of the flat portion  14   a  is preferably 0.02 to 0.5 mm, and more preferably 0.02 to 0.2 mm. 
     Next will be described an assembling method of the porous membrane F, the barrel  10 , and the cap  20  thus described, referring to  FIG. 4  and  FIG. 5  showing an assembling process of the porous membrane cartridge  1 . 
     As shown in  FIG. 4 , dispose the porous membrane F so as to be put on the bottom portion  26  of the cap  20 . At this time, because the sandwiching face  24  and the ribs  25  are designed higher than the bottom portion  26  by one step, the porous membrane F is, to be more precise, put on the sandwiching face  24  and the ribs  25 . Then because the diameter of the porous membrane F is approximately same as the inner diameter of the cap  20 , as shown in  FIG. 5(   a ), a peripheral edge Fa of the porous membrane F results in being positioned on the sandwiching face  24 . 
     Then fitting the top end  13  of the barrel  10  inside the fit-in portion  22  of the cap  20 , make the opening edge  14  of the barrel  10  abut with the peripheral edge Fa of the porous membrane F. In such a temporal assembly, perform welding by an ultrasonic welder not shown. For example, set the cap  20  on a platform for pressurizing of the ultrasonic welder, apply an ultrasound while pressurizing the barrel  10  toward the cap  20  by horn. 
     Whereat, by pressing the barrel  10  to the cap  20 , an air gap of the porous membrane F is crushed, and then as shown in  FIG. 5(   b ), the peripheral edge Fa of the outmost perimeter of the porous membrane F is crushed. At this time, because the flat portion  14   a  provided at the outmost perimeter of the opening edge  14  sandwiches the porous membrane F between itself and the sandwiching face  24 , a position of the membrane F is decided, and an unnecessary positional displacement of the membrane F is prevented. Then part of the porous membrane F is further crushed as shown in  FIG. 5(   c ) while melting, and the opening edge  14  abuts with the sandwiching face  24 . Subsequently, a peripheral edge including the flat portion  14   a  of the opening edge  14  and that of the sandwiching face  24  melt and are welded. As a result, as shown in  FIG. 5(   d ), the opening edge  14  of the barrel  10  and the sandwiching face  24  of the cap  20  sandwich the porous membrane F, and the membrane F is held in a state of its peripheral edge Fa being crushed. Also the porous membrane F existing between the taper portion  14   b  of the opening edge  14  and the sandwiching face  24  is also compressed by the taper portion  14   b  and part of an air gap is crushed. Then the end  14   c  is in contact with the surface of the porous membrane F. At this time, because the end  14   c  is formed as a blunt angle by the chamfer  15 , it is difficult to damage the porous membrane F. 
     The porous membrane cartridge  1  thus made is used as follows: 
     Firstly, as sample solutions containing nucleic acids, prepare body fluids such as a whole blood, plasma, serum, urine, human waste, semen, and saliva taken as analytes; or solutions adjusted from biotic materials such as a soluble matter and homogenate of a vegetable (or its part), an animal (or its part), and the like. Treat these solutions with a water solution containing a reagent, which solves a cell membrane and solublizes the nucleic acids. Thus the cell membrane and nucleic membrane are solved, and the nucleic acids are dispersed in the water solution. For example, when a sample is a whole blood, red blood cells and various proteins are removed and white blood cells and nucleic membranes are solved by incubation of 10 minutes at 60 degrees Celsius in a state of addition of Guanidine Hydrochloride, Triton-X100, and Protease K (manufactured by SIGMA Corp.). 
     Adding a water soluble organic solvent, for example, ethanol to a water solution where nucleic acids are thus dispersed, a sample solution is completed. Pass the sample solution while applying a pressure from the opening  11   b  at the rear end side of the barrel  10  toward the opening  21   a  of the top of the nozzle  23 . Thus the nucleic acids in the sample solution are adsorbed to the porous membrane F. 
     In a pressurizing system of passing a sample solution by applying the pressure with being compared to a centrifugal separation system that passes the solution by centrifugal force, although the solution attempts to flow toward the peripheral edge Fa of the porous membrane F, because in the portion Fa of the porous membrane F the end portion  14   c  of the barrel  10  is in contact with the surface of the membrane F, the solution cannot enter a space between the membrane F and the opening edge  14  of the barrel  10 . In addition, because in the peripheral edge Fa of the porous membrane F an air gap is crushed by the taper portion  14   b  and the sandwiching face  24  and furthermore the outer perimeter portion thereof is completely closed by welding of the barrel  10  and the cap  20 , it does not occur that a sample solution goes around a side portion (edge of the outer perimeter) of the membrane F. Accordingly, nucleic acids in a sample solution result in being adsorbed only to an inside portion surrounded by the end  14   c  of the barrel  10  out of the porous membrane F. 
     Next, pass a nucleic acid washing buffer while applying a pressure from the opening  11   b  at the rear end side of the porous membrane cartridge  1  toward the opening  21   a  of the nozzle  23 . Although the nucleic acid washing buffer has a composition of desorbing not nucleic acids adsorbed to the porous membrane F but impurities, it is composed of a water solution containing a main agent and a buffer agent, and a surfactant as needed. As the main agent, a solution containing ethanol, Tris, and Triton-X100 is preferable. By this operation, impurities other than nucleic acids are removed from the porous membrane F. 
     At this time, out of the porous membrane F, because a nucleic acid washing buffer sufficiently passes a portion where the sample solution passed, that is, a portion surrounded by the end  14   c , impurities are removed without remaining at the peripheral edge Fa of the membrane F. 
     Next, passing any one of such refined distilled water and a TE buffer (containing Tris and EDTA (ethylenediamine tetraacetic acid)) while applying a pressure from the opening  11   b  toward the opening  21   a  (see  FIG. 2 ), desorb nucleic acids from the porous membrane F, desorb the acids and make them flow out, and recover a solution containing the flowed-out nucleic acids. At this time because same as in adsorbing nucleic acids to the porous membrane F, such refined distilled water sufficiently passes through a portion where the acids surrounded by the end  14   c  out of the porous membrane F, the acids are sufficiently desorbed without remaining at the peripheral edge Fa of the membrane F. 
     Thus in the porous membrane cartridge  1  of the embodiment, because when passing such a sample solution where nucleic acids is dispersed, a nucleic acid washing buffer, and refined distilled water, they do not go around a side portion of the porous membrane F, it does not occur that: the acids are discharged without being adsorbed to the membrane F, and impurities mix in a solution where the acids are recovered. In addition, because the end  14   c  is in contact with the porous membrane F and it does not also occur that the various liquids described above enter a space between the membrane F and the opening edge  14  of the barrel  10 , impurities do not mix in a nucleic acid solution after the recovery, and a nucleic acid recovery efficiency is also higher. 
     In addition, in the porous membrane cartridge  1  of the embodiment, because part of the opening edge  14  is formed so as to be the taper portion  14   b  where an inner perimeter side is more retreated than an outer perimeter side and a sandwiching force by the opening edge  14  and the sandwiching face  24  gradually becomes smaller from an outer perimeter to an inner perimeter, it is enabled to avoid a concentration of a stress applied to the porous membrane F. In addition, because that the opening edge  14  is designed to be a blunt angle also alleviates the concentration of the stress applied to the porous membrane F, it is enabled to prevent the membrane F from breaking. 
     In addition, in a case of using the porous membrane cartridge  1  in filtration, because a liquid does not go around the side portion of the porous membrane F, mixing of impurities in a liquid after the filtration becomes less. 
     The first embodiment thus described is one example of the present invention, and because it suffices that the invention may fix the barrel  10  and the cap  20  in a state of crushing and sandwiching the porous membrane F, various variation examples as below can be considered. Meanwhile, in embodiments below with respect to a same portion as in the first embodiment, a detailed description thereof will be omitted, appending a same symbol. 
     Second Embodiment 
       FIG. 6  is drawings showing a porous membrane cartridge  1 A related to a second embodiment of the present invention, and is section views corresponding to the enlarged view of the part A in  FIG. 2 . As shown in  FIG. 6(   a ), in the porous membrane cartridge  1 A of the second embodiment a bead  16   a  of which a section is chevron is provided across a whole perimeter in the vicinity of an outer perimeter of an opening edge  16  of the barrel  10 . Then an inner perimeter side of the bead  16   a  is designed to be a flat portion  16   b  for orthogonalizing an axial line of the barrel  10 . 
     In a case of assembling such the porous membrane cartridge  1 A, disposing the porous membrane F at the bottom portion  26  of the cap  20  and fitting the top end  13  of the barrel  10  in the fit-in portion  22  of the cap  20 , sandwich the membrane F between the opening edge  16  and the sandwiching face  24 . 
     Then if applying an ultrasound to the barrel  10  by horn while pressing the opening edge  16  to the sandwiching face  24  in order to weld the cap  20  and the barrel  10  by ultrasonic welder, the bead  16   a  melts and penetrates the porous membrane F, and as shown in  FIG. 6(   b ), the sandwiching face  24  and the bead  16   a  are welded. If further making the welding proceed, the flat portion  16   b  crushes and sandwiches the porous membrane F between itself and the sandwiching face  24  as shown in  FIG. 6(   c ). 
     Also in the porous membrane cartridge  1 A thus made, because the opening edge  16  and the sandwiching face  24  are welded while the peripheral edge Fa of the porous membrane F melts, a liquid does not go around a side portion of the porous membrane F. In addition, because the porous membrane F is sandwiched between the flat portion  16   b  and the sandwiching face  24  while crushed between them, a liquid does not enter the portion Fa of the membrane F. 
     Accordingly, also in the porous membrane cartridge  1 A of the embodiment, a recovery efficiency of nucleic acids is better and mixing of impurities in a solution after a recovery is less. 
     In addition, in a case of using the porous membrane cartridge  1 A in filtration, mixing of impurities in a liquid after the filtration becomes less. 
     Meanwhile, in a case of providing such a bead are available not only a mode of providing the bead at the opening edge  16  of the barrel  10  but also a porous membrane cartridge  1 C of a mode where a bead  24   b  of which a section is chevron across a whole perimeter in the vicinity of the outer perimeter of the sandwiching face  24  without providing the bead at the opening edge  16  as shown in  FIG. 7 . 
     Third Embodiment 
       FIG. 8  is drawings showing a porous membrane cartridge  1 D related to a third embodiment of the present invention and corresponding to the enlarged view of the part A in  FIG. 2 . The porous membrane cartridge  1 D is a mode of welding the barrel  10  and the cap  20  at a portion of an outer perimeter more than the sandwiching face  24 . 
     In other words, as shown in  FIG. 8(   a ), in the cap  20  is formed a step portion  27  for welding defined by: a wall face portion  27   a  perpendicularly rising up with respect to the sandwiching face  24  at outside of the sandwiching face  24 ; and a slant portion  27   b  slantedly rising up toward the inner perimeter of the fit-in portion  22  from an upper end (namely, the rear end) in  FIG. 8(   a ) of the wall face portion  27   a.    
     On the other hand, at an opening edge  17  of the barrel  10  are formed a flat portion  17   a  parallel with the sandwiching face  24  formed at an inner perimeter side; a wall face portion  17   b  composed of a peripheral face for fitting in the wall face portion  27   a  formed with continuing from an outer perimeter end of the flat portion  17   a ; a slant portion  17   c  formed with continuing from the top end in  FIG. 8(   a ) of the wall face portion  27   a  and parallel with the slant portion  27   b ; and a flat portion  17   d  formed with continuing into the slant portion  17   c  and extending toward the outer perimeter of the barrel  10 . 
     If such the porous membrane cartridge  1 D sandwiches the porous membrane F between the sandwiching face  24  and the flat portion  17   a , and the barrel  10  and the cap  20  is applied to an ultrasonic welder, the flat portion  17   d  abuts with the slant portion  27   b  and the abutment portion is welded. Then the peripheral edge Fa of the porous membrane F is sandwiched between the flat portion  17   a  and the sandwiching face  24  in a state of being crushed. 
     Thus only sandwiching and holding the porous membrane F and welding the barrel  10  and the cap  20  at an outer portion more than the membrane F, it does not occur to damage the membrane F also in a case that it is especially delicate. 
     Furthermore, as a mode of fixing the barrel  10  and the cap  20  at outside of the porous membrane F are cited modes shown in  FIG. 9 . 
     For example, as shown in  FIG. 9(   a ), it is also enabled to make the main body  12  of the barrel  10  and a face of a step difference of an outer perimeter face of the top end  13  a joint portion  18  for welding, on the other hand to form a protrusion abutting with the portion  18  across a whole perimeter at an opening edge  28  at a rear end side of the cap  20 , and to thus make a porous membrane cartridge  1 E where the joint portion  18  and the opening edge  28  are welded. 
     In addition, as shown in  FIG. 9(   b ), form a bead  19  composed of a gentle circular section along a peripheral direction across a whole perimeter at an outer perimeter of the top end  13  of the barrel  10 , and on the other hand, in advance form a corresponding depression groove  29  at a portion corresponding to the bead  19  on an inner perimeter face of the fit-in portion  22  of the cap  20 . Then by an engagement between the bead  19  and the depression groove  29 , it is enabled to sandwich the porous membrane F between the opening edge  14  and the sandwiching face  24  in a state of crushing the outer perimeter edge of the membrane F. 
     Because such a porous membrane cartridge  1 F is enabled to be assembled by hand, it is convenient in small production. 
     Thus, although the embodiments of the present invention are described, the invention is not limited thereto, and it can be carried out with being appropriately varied. 
     For example, the barrel  10  and the cap  20  can also be made to adhere by any one of an adhesive and a UV curing resin, and the outer perimeter face of the top end  13  of the barrel  10  and the inner perimeter face of the fit-in portion  22  of the cap  20  can also be welded by laser. Meanwhile, in a case of laser welding, it is preferable to make the barrel  10  a material that can generate heat by absorbing laser or to blend a plasticizer in the barrel  10 . 
     Example 
     Next will be described one example of the present invention. 
     In the example, manufacturing a porous membrane cartridge of a shape in the first embodiment and changing a welding condition at that time, a test for determining whether welding was good or bad was performed. 
     As a material of the barrel  10  and the cap  20  was used polystyrene (manufactured by A&amp;M Styrene Co., Ltd.). The outer diameter of the opening edge  14  of the barrel  10  was made 7 mm, the width of the flat portion  14   a  at outer perimeter side of opening edge  14  was made 0.05 mm, and as shown in  FIG. 5(   a ), the angle of the taper portion  14   b  was formed to be 10 degrees for the flat portion  14   a.    
     As the porous membrane F was used triacetylcellulose (Microfilter FM500 manufactured by Fuji Photo Film Co., Ltd.). 
     Using an ultrasonic welder (40 KHz, 800 KW) manufactured by Branson Corp. as an ultrasonic welder, disposing the porous membrane F at the bottom portion  26  of the cap  20  same as in the first embodiment, and fitting the top end  13  of the barrel  10  in the cap  20 , the barrel  10  was vibrated while pressed toward the cap  20  by a horn of the ultrasonic welder. Changing a pressing force and a vibration time at this time, a welding result under each condition was determined. 
     Table 1 shows the result. 
     
       
         
           
               
               
             
               
                   
                 TABLE 1 
               
             
            
               
                   
                   
               
               
                   
                 Pressing Force (N) 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                 50 
                 75 
                 100 
                 150 
                 200 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 Vibration 
                 0.03 
                 X 
                 X 
                 X 
                 X 
                 X 
               
               
                   
                 Time (sec) 
                 0.05 
                 X 
                 X 
                 ◯ 
                 ◯ 
                 ◯ 
               
               
                   
                   
                 0.1 
                 X 
                 X 
                 ◯ 
                 ◯ 
                 ◯ 
               
               
                   
                   
                 0.15 
                 X 
                 ◯ 
                 ◯ 
                 ◯ 
                 ◯ 
               
               
                   
                   
                 0.2 
                 Δ 
                 ◯ 
                 ◯ 
                 ◯ 
                 ◯ 
               
               
                   
                   
                 0.25 
                 Δ 
                 ◯ 
                 ◯ 
                 ◯ 
                 ◯ 
               
               
                   
                   
                 0.3 
                 Δ 
                 ◯ 
                 ◯ 
                 ◯ 
                 □ 
               
               
                   
                   
                 0.35 
                 Δ 
                 ◯ 
                 ◯ 
                 ◯ 
                 □ 
               
               
                   
                   
                 0.4 
                 Δ 
                 ◯ 
                 ◯ 
                 □ 
                 □ 
               
               
                   
                   
                 0.45 
                 Δ 
                 ◯ 
                 ◯ 
                 □ 
                 □ 
               
               
                   
                   
                 0.5 
                 Δ 
                 □ 
                 □ 
                 □ 
                 □ 
               
               
                   
                   
                 0.6 
                 Δ 
                 □ 
                 □ 
                 □ 
                 □ 
               
               
                   
                   
               
               
                   
                 X Unable to be Welded 
               
               
                   
                 Δ Liquid Leakage 
               
               
                   
                 □ Perforation of Porous Membrane and Deformation of Molded Product 
               
               
                   
                 ◯ Good 
               
            
           
         
       
     
     As shown in Table 1 were changed the pressing force between 50 N and 200 N and the vibration time between 0.03 sec and 0.6 sec. In Table 1, the symbol x indicates that the barrel  10  and the cap  20  could not be welded; the symbol Δ indicates that although the barrel  10  and the cap  20  could be welded, the welding was insufficient and there was a liquid leakage from the welded portion; the symbol □ indicates that there was a defect that the porous membrane F was perforated or a formed product was deformed; and the symbol O indicates that the welding could be performed well without the defect. 
     As seen from Table 1, in a case of the pressing force of 50 N, the welding could not be performed or there was a liquid leakage, and any case of the vibration time was bad. 
     In a case of the pressing force of 75 N, the welding could not be performed in a case of the vibration time not more than 0.1 sec and could be performed well in a case of the vibration time between 0.15 sec and 0.45 sec; in a case of the vibration time not less than 0.5 sec, a welding time was too long and there was the defect in a formed product. 
     In a case of the pressing force of 100 N, the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.45 sec; in a case of the vibration time not less than 0.5 sec, a welding time was too long and there was the defect in a formed product. 
     In a case of the pressing force of 150 N, the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.35 sec; in a case of the vibration time not less than 0.4 sec, a welding time was too long and there was the defect in a formed product. 
     In a case of the pressing force of 200 N, the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.25 sec; in a case of the vibration time not less than 0.3 sec, a welding time was too long and there was the defect in a formed product. 
     Thus in a case of the pressing force between 75 N and 200 N, good welding was enabled if between a predetermined vibration time, and as a result, it was enabled to manufacture a preferable porous membrane cartridge where a liquid leakage does not occur.