Patent Publication Number: US-2022220117-A1

Title: 9-substituted amino triazolo quinazoline derivatives as adenosine receptor antagonists, pharmaceutical compositions and their use

Description:
FIELD OF THE INVENTION 
     The present invention relates to novel compounds that inhibit at least one of the A2a and A2b adenosine receptors, and pharmaceutically acceptable salts thereof, and compositions comprising such compound(s) and salts, methods for the synthesis of such compounds, and their use in the treatment of a variety of diseases, conditions, or disorders that are mediated, at least in part, by the adenosine A2a receptor and/or the adenosine A2b receptor. Such diseases, conditions, and disorders include but are not limited to cancer and immune-related disorders. The invention further relates to combination therapies, including but not limited to a combination comprising a compound of the invention and a PD-1 antagonist. 
     BACKGROUND OF THE INVENTION 
     Adenosine is a purine nucleoside compound comprised of adenine and ribofuranose, a ribose sugar molecule. Adenosine occurs naturally in mammals and plays important roles in various biochemical processes, including energy transfer (as adenosine triphosphate and adenosine monophosphate) and signal transduction (as cyclic adenosine monophosphate). Adenosine also plays a causative role in processes associated with vasodilation, including cardiac vasodilation. It also acts as a neuromodulator (e.g., it is thought to be involved in promoting sleep). In addition to its involvement in these biochemical processes, adenosine is used as a therapeutic antiarrhythmic agent to treat supraventricular tachycardia and other indications. 
     The adenosine receptors are a class of purinergic G protein-coupled receptors with adenosine as the endogenous ligand. The four types of adenosine receptors in humans are referred to as A1, A2a, A2b, and A3. Modulation of A1 has been proposed for the management and treatment of neurological disorders, asthma, and heart and renal failure, among others. Modulation of A3 has been proposed for the management and treatment of asthma and chronic obstructive pulmonary diseases, glaucoma, cancer, stroke, and other indications. Modulation of the A2a and A2b receptors are also believed to be of potential therapeutic use. 
     In the central nervous system, A2a antagonists are believed to exhibit antidepressant properties and to stimulate cognitive functions. A2a receptors are present in high density in the basal ganglia, known to be important in the control of movement. Hence, A2a receptor antagonists are believed to be useful in the treatment of depression and to improve motor impairment due to neurodegenerative diseases such as Parkinson&#39;s disease, senile dementia (as in Alzheimer&#39;s disease), and in various psychoses of organic origin. 
     In the immune system, adenosine signaling through A2a receptors and A2b receptors, expressed on a variety of immune cells and endothelial cells, has been established as having an important role in protecting tissues during inflammatory responses. In this way (and others), tumors have been shown to evade host responses by inhibiting immune function and promoting tolerance. (See, e.g., Fishman, P., et al., Handb. Exp. Pharmacol. (2009) 193:399-441). Moreover, A2a and A2b cell surface adenosine receptors have been found to be upregulated in various tumor cells. Thus, antagonists of the A2a and/or A2b adenosine receptors represent a new class of promising oncology therapeutics. For example, activation of A2a adenosine receptors results in the inhibition of the immune response to tumors by a variety of cell types, including but not limited to: the inhibition of natural killer cell cytotoxicity, the inhibition of tumor-specific CD4+/CD8+ activity, promoting the generation of LAG-3 and Foxp3+ regulatory T-cells, and mediating the inhibition of regulatory T-cells. Adenosine A2a receptor inhibition has also been shown to increase the efficacy of PD-1 inhibitors through enhanced anti-tumor T cell responses. As each of these immunosuppressive pathways has been identified as a mechanism by which tumors evade host responses, a cancer immunotherapeutic regimen that includes an antagonist of the A2a and/or A2b receptors, alone or together with one or more other therapeutic agents designed to mitigate immune suppression, may result in enhanced tumor immunotherapy. (See, e.g., P. Beavis, et al., Cancer Immunol. Res. DOI: 10.1158/2326-6066. CIR-14-0211, Feb. 11, 2015; Willingham, S B., et al., Cancer Immunol. Res., 6(10), 1136-49; and Leone R D, et al., Cancer Immunol. Immunother., August 2018, Vol. 67, Issue 8, 1271-1284). 
     Cancer cells release ATP into the tumor microenvironment when treated with chemotherapy and radiation therapy, which is subsequently converted to adenosine. (See Martins, I., et al., Cell Cycle, vol. 8, issue 22, pp. 3723 to 3728.) The adenosine can then bind to A2a receptors and blunt the anti-tumor immune response through mechanisms such as those described above. The administration of A2a receptor antagonists during chemotherapy or radiation therapy has been proposed to lead to the expansion of the tumor-specific T-cells while simultaneously preventing the induction of tumor-specific regulatory T-cells. (Young, A., et al., Cancer Discovery (2014) 4:879-888). 
     The combination of an A2a receptor antagonist with anti-tumor vaccines is believed to provide at least an additive therapeutic effect in view of their different mechanisms of action. Further, A2a receptor antagonists may be useful in combination with checkpoint blockers. By way of example, the combination of a PD-1 inhibitor and an adenosine A2a receptor inhibitor is thought to mitigate the ability of tumors to inhibit the activity of tumor-specific effector T-cells. (See, e.g., Willingham, S B., et al., Cancer Immunol. Res.; 6(10), 1136-49; Leone, R D., et al., Cancer Immunol. Immunother., August 2018, Vol. 67, Issue 8, pp. 1271-1284; Fishman, P., et al., Handb. Exp. Pharmacol. (2009) 193:399-441; and Sitkovsky, M V., et al., (2014) Cancer Immunol. Res 2:598-605.) 
     The A2b receptor is a G protein-coupled receptor found in various cell types. A2b receptors require higher concentrations of adenosine for activation than the other adenosine receptor subtypes, including A2a. (Fredholm, B B., et al., Biochem. Pharmacol. (2001) 61:443-448). Conditions which activate A2b have been seen, for example, in tumors where hypoxia is observed. The A2b receptor may thus play an important role in pathophysiological conditions associated with massive adenosine release. While the pathway(s) associated with A2b receptor-mediated inhibition are not well understood, it is believed that the inhibition of A2b receptors (alone or together with A2a receptors) may block pro-tumorigenic functions of adenosine in the tumor microenvironment, including suppression of T-cell function and angiogenesis, and thus expand the types of cancers treatable by the inhibition of these receptors. 
     A2b receptors are expressed primarily on myeloid cells. The engagement of A2b receptors on myeloid derived suppressor cells (MDSCs) results in their expansion in vitro (Ryzhov, S. et al., J. Immunol. 2011, 187:6120-6129). MDSCs suppress T-cell proliferation and anti-tumor immune responses. Selective inhibitors of A2b receptors and A2b receptor knockouts have been shown to inhibit tumor growth in mouse models by increasing MDSCs in the tumor microenvironment (Iannone, R., et al., Neoplasia Vol. 13 No. 12, (2013) pp. 1400-1409; Ryzhov, S., et al., Neoplasia (2008) 10: 987-995). Thus, A2b receptor inhibition has become an attractive biological target for the treatment of a variety of cancers involving myeloid cells. Examples of cancers that express A2b receptors can be readily obtained through analysis of the publicly available TCGA database. Such cancers include lung, colorectal, head and neck, and cervical cancer, among others, and are discussed in further detail below. 
     Angiogenesis plays an important role in tumor growth. The angiogenesis process is highly regulated by a variety of factors and is triggered by adenosine under particular circumstances that are associated with hypoxia. The A2b receptor is expressed in human microvascular endothelial cells, where it plays an important role in the regulation of the expression of angiogenic factors such as the vascular endothelial growth factor (VEGF). In certain tumor types, hypoxia has been observed to cause an upregulation of the A2b receptors, suggesting that inhibition of A2b receptors may limit tumor growth by limiting the oxygen supply to the tumor cells. Furthermore, experiments involving adenylate cyclase activation indicate that A2b receptors are the sole adenosine receptor subtype in certain tumor cells, suggesting that A2b receptor antagonists may exhibit effects on particular tumor types. (See, e.g., Feoktistov, I., et al., (2003) Circ. Res. 92:485-492; and P. Fishman, P., et al., Handb. Exp. Pharmacol. (2009) 193:399-441). 
     In view of their promising and varied therapeutic potential, there remains a need in the art for potent and selective inhibitors of the A2a and/or A2b adenosine receptors, for use alone or in combination with other therapeutic agents. The present invention addresses this and other needs. 
     SUMMARY OF THE INVENTION 
     In one aspect, the present invention provides compounds (hereinafter referred to as compounds of the invention) which, surprisingly and advantageously, have been found to be inhibitors of the adenosine A2a receptor and/or the adenosine A2b receptor. The compounds of the invention have a structure in accordance with the structural Formula (I): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein ring A, R 1 , and R 2  are as defined below. 
     In another aspect, the present invention provides pharmaceutical compositions comprising at least one compound of the invention, or a pharmaceutically acceptable salt thereof, in a pharmaceutically acceptable carrier or diluent. Such compositions according to the invention may optionally further include one or more additional therapeutic agents as described herein. 
     In another aspect, the present invention provides a method for treating or preventing a disease, condition, or disorder that is mediated, at least in part, by the adenosine A2a receptor and/or the adenosine A2b receptor in a subject (e.g., an animal or human) in need thereof, said method comprising administering to the subject a therapeutically effective amount of at least one compound of the invention, or a pharmaceutically acceptable salt thereof, alone or in combination with one or more additional therapeutic agents. These and other aspects and embodiments of the invention are described more fully below. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     For each of the following embodiments, any variable not explicitly defined in the embodiment is as defined in Formula (I). In each of the embodiments described herein, each variable is selected independently of the other unless otherwise noted. 
     In one embodiment, the compounds of the invention have the structural Formula (I): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     R 1  is selected from F, Cl, (C 1 -C 6 )alkyl, and O(C 1 -C 6 )alkyl; 
     R 2  is selected from H, F, Cl, (C 1 -C 6 )alkyl, and O(C 1 -C 6 )alkyl; 
     ring A is a moiety selected from: 
     
       
         
         
             
             
         
       
     
     R 3  is selected from pyrazolyl, triazolyl, and pyridinyl, wherein said pyrazolyl and said triazolyl, are substituted with 1 or 2 R 3A  groups, and wherein said pyridinyl is substituted with 1, 2, or 3 R 3A  groups, wherein: 
     each R 3A  is independently selected from (C 1 -C 6 )alkyl, O(C 1 -C 6 )alkyl, (C 1 -C 6 )alkyl-OH, (C 1 -C 6 )haloalkyl, O(C 1 -C 6 )haloalkyl, oxo, (C 1 -C 4 )alkylC(O)(C 1 -C 3 )alkyl, (C 1 -C 4 )alkylCH(OH)(C 1 -C 3 )alkyl, (C 1 -C 4 )alkylS(O) 2 (C 1 -C 3 )alkyl, —(CH 2 ) n (C 3 -C 7 )cycloalkyl, and —(CH 2 ) n 4-7 membered monocyclic heterocycloalkyl comprising 1 or 2 ring heteroatoms selected from oxygen and nitrogen, wherein said (C 3 -C 7 )cycloalkyl, and said 4-7 membered monocyclic heterocycloalkyl are each unsubstituted or substituted with 1, 2, or 3 groups independently selected from F, Cl, OH, (C 1 -C 6 )alkyl, and (C 1 -C 6 )haloalkyl; 
     n is 0, 1, or 2; 
     R A1  is selected from H, and (C 1 -C 4 )alkyl; 
     R A2  is selected from H, F, and (C 1 -C 4 )alkyl; 
     R A3  is selected from H, F, and (C 1 -C 4 )alkyl; 
     R A4  is selected from H and OH; and 
     R A5  is selected from H, F, and (C 1 -C 4 )alkyl. 
     In another embodiment, the compounds of the invention have the structural Formula (I.1): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein ring A, R 1 , and R 2  are as defined in Formula (I). 
     In another embodiment, the compounds of the invention have the structural Formula (I.2): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein ring A, R 1 , and R 2  are as defined in Formula (I). 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     R 1  is selected from F, Cl, and OCH 3 ; 
     R 2  is selected from H, F, Cl, CH 3 , and OCH 3 . 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     R 1  is F; and 
     R 2  is selected from H, F, Cl, CH 3 , and OCH 3 . 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     R 1  is Cl; and 
     R 2  is selected from H, F, Cl, CH 3 , and OCH 3 . 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     R 1  is F; and 
     R 2  is OCH 3 . 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     R 1  is F; and 
     R 2  is F. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     R 1  is F; and 
     R 2  is H. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is a moiety selected from: 
     
       
         
         
             
             
         
       
     
     wherein R 3 , R A1 , R A2 , R A3 , and R A5  are as defined in Formula (I); and wherein R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is a moiety selected from: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein: 
     each R 3A  is as defined in Formula (I); 
     each R Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; 
     R A1 , R A2 , R A3 , and R A5  are as defined in Formula (I); and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is a moiety selected from: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein:
         each R 3A  is a moiety selected from:       

     
       
         
         
             
             
         
       
     
     each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; 
     R A1 , R A2 , R A3 , and R A5  are as defined in Formula (I); and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In an alternative of the immediately preceding embodiment: 
     R A1  is selected from H, CH 3 , and CH 2 CH 3 ; 
     R A2  is selected from H, F, CH 3 , and CH 2 CH 3 ; 
     R A3  is selected from H and F; and 
     R A5  is H. 
     In another alternative of the immediately preceding embodiment: 
     R A1  is selected from H and CH 3 ; 
     R A2  is H; 
     R A3  is H; and 
     R A5  is H. 
     In another alternative of the immediately preceding embodiment: 
     R A1  is H; 
     R A2  is H; 
     R A3  is H; and 
     R A5  is H. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein R 3  and R A3  are as defined in Formula (I); and wherein R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein:
         each R 3A  is as defined in Formula (I);   each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl;       

     R A3  is as defined in Formula (I); and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein: 
     each R 3A  is a moiety selected from: 
     
       
         
         
             
             
         
       
     
     each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; 
     R A3  is as defined in Formula (I); and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In an alternative of the immediately preceding embodiment: 
     R A3  is selected from H and F. 
     In another alternative of the immediately preceding embodiment: 
     R A3  is H. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein R 3  is as defined in Formula (I); and wherein R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     
       
         
         
             
             
         
       
     
     R 3  is selected from 
     wherein: 
     each R 3A  is as defined in Formula (I); 
     each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein: 
     each R 3A  is a moiety selected from: 
     
       
         
         
             
             
         
       
     
     each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein R 3  is as defined in Formula (I); and wherein R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein: 
     each R 3A  is as defined in Formula (I); 
     each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein: 
     each R 3A  is a moiety selected from: 
     
       
         
         
             
             
         
       
     
     each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein R 3 , R A1 , R A2 , and R A3  are as defined in Formula (I); and wherein R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein: 
     each R 3A  is as defined in Formula (I); 
     each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; 
     R A1 , R A2 , and R A3  are as defined in Formula (I); and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein: 
     each R 3A  is a moiety selected from: 
     
       
         
         
             
             
         
       
     
     each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; 
     R A1 , R A2 , and R A3  are as defined in Formula (I); and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In an alternative of the immediately preceding embodiment: 
     R A1  is selected from H, CH 3 , and CH 2 CH 3 ; 
     R A2  is selected from H, F, CH 3 , and CH 2 CH 3 ; and 
     R A3  is selected from H and F. 
     In another alternative of the immediately preceding embodiment: 
     R A1  is selected from H and CH 3 ; 
     R A2  is H; and 
     R A3  is H. 
     In another alternative of the immediately preceding embodiment: 
     R A1  is H; 
     R A2  is H; and 
     R A3  is H. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein R 3 , R A1 , R A2 , and R A3  are as defined in Formula (I); and 
     wherein R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein:
         each R 3A  is as defined in Formula (I);   each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl;       

     R A1 , R A2 , and R A3  are as defined in Formula (I); and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein: 
     each R 3A  is a moiety selected from: 
     
       
         
         
             
             
         
       
     
     each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; 
     R A1 , R A2 , and R A3  are as defined in Formula (I); and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In an alternative of the immediately preceding embodiment: 
     R A1  is selected from H, CH 3 , and CH 2 CH 3 ; 
     R A2  is selected from H, F, CH 3 , and CH 2 CH 3 ; and 
     R A3  is selected from H and F. 
     In another alternative of the immediately preceding embodiment: 
     R A1  is selected from H and CH 3 ; 
     R A2  is H; and 
     R A3  is H. 
     In another alternative of the immediately preceding embodiment: 
     R A1  is H; 
     R A2  is H; and 
     R A3  is H. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein R 3 , R A1 , R A2 , R A3  and R A4  are as defined in Formula (I); and 
     wherein R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein: 
     each R 3A  is as defined in Formula (I); 
     each R 3Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )halo alkyl; 
     R A1 , R A2 , R A3  and R A4  re as defined in Formula (I); and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In another embodiment, in each of Formulas (I), (I.1), and (I.2): 
     ring A is the moiety: 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 3  is selected from 
     
       
         
         
             
             
         
       
     
     wherein: 
     each R 3A  is a moiety selected from: 
     
       
         
         
             
             
         
       
     
     each R Aa  is independently selected from (C 1 -C 4 )alkyl, O(C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, and O(C 1 -C 4 )haloalkyl; 
     R A1 ; R A2 ; R A3  and R A4  re as defined in Formula (I); and 
     R 1  and R 2  are as defined in Formula (I) or as defined in any of the alternative embodiments of R 1  and R 2  described above. 
     In an alternative of the immediately preceding embodiment: 
     R A1  is selected from H, CH 3 , and CH 2 CH 3 ; 
     R A2  is selected from H, F, CH 3 , and CH 2 CH 3 ; 
     R A3  is selected from H and F; and 
     R A4  is selected from H and OH. 
     In an alternative of the immediately preceding embodiment: 
     R A1  is H; 
     R A2  is H; 
     R A3  is H; and 
     R A4  is selected from H and OH. 
     In another embodiment, the compounds of the invention comprise those compounds identified herein as examples in the tables below, and pharmaceutically acceptable salts thereof. 
     In another aspect, the present invention provides pharmaceutical compositions comprising a pharmaceutically acceptable carrier and a compound of the invention or a pharmaceutically acceptable salt thereof. Such compositions according to the invention may optionally further include one or more additional therapeutic agents as described herein. 
     In another aspect, the present invention provides a method for the manufacture of a medicament or a composition which may be useful for treating diseases, conditions, or disorders that are mediated, at least in part, by the adenosine A2a receptor and/or the adenosine A2b receptor, comprising combining a compound of the invention with one or more pharmaceutically acceptable carriers. 
     In another aspect, the present invention provides a method for treating or preventing a disease, condition, or disorder that is mediated, at least in part, by the adenosine A2a receptor and/or the adenosine A2b receptor in a subject (e.g., an animal or human) in need thereof, said method comprising administering to the subject in need thereof a therapeutically effective amount of at least one compound of the invention, or a pharmaceutically acceptable salt thereof, alone or in combination with one or more additional therapeutic agents. Specific non-limiting examples of such diseases, conditions, and disorders are described herein. 
     Oncology 
     In some embodiments, the disease, condition or disorder is a cancer. Any cancer for which a PD-1 antagonist and/or an A2a and/or A2b inhibitor are thought to be useful by those of ordinary skill in the art are contemplated as cancers treatable by this embodiment, either as a monotherapy or in combination with other therapeutic agents discussed below. Cancers that express high levels of A2a receptors or A2b receptors are among those cancers contemplated as treatable by the compounds of the invention. Examples of cancers that express high levels of A2a and/or A2b receptors may be discerned by those of ordinary skill in the art by reference to the Cancer Genome Atlas (TCGA) database. Non-limiting examples of cancers that express high levels of A2a receptors include cancers of the kidney, breast, lung, and liver. Non-limiting examples of cancers that express high levels of the A2b receptor include lung, colorectal, head &amp; neck cancer, and cervical cancer. 
     Thus, one embodiment provides a method of treating cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a subject in need of such treatment, wherein said cancer is a cancer that expresses a high level of A2a receptor. A related embodiment provides a method of treating cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a subject in need of such treatment, wherein said cancer is selected from kidney (or renal) cancer, breast cancer, lung cancer, and liver cancer. 
     Another embodiment provides a method of treating cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a subject in need of such treatment, wherein said cancer is a cancer that expresses a high level of A2b receptor. A related embodiment provides a method of treating cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a subject in need of such treatment, wherein said cancer is selected from lung cancer, colorectal cancer, head &amp; neck cancer, and cervical cancer. 
     Additional non-limiting examples of cancers which may be treatable by administration of a compound of the invention (alone or in combination with one or more additional agents described below) include cancers of the prostate (including but not limited to metastatic castration resistant prostate cancer), colon, rectum, pancreas, cervix, stomach, endometrium, brain, liver, bladder, ovary, testis, head, neck, skin (including melanoma and basal carcinoma), mesothelial lining, white blood cell (including lymphoma and leukemia) esophagus, breast, muscle, connective tissue, lung (including but not limited to small cell lung cancer, non-small cell lung cancer, and lung adenocarcinoma), adrenal gland, thyroid, kidney, or bone. Additional cancers treatable by a compound of the invention include glioblastoma, mesothelioma, renal cell carcinoma, gastric carcinoma, sarcoma, choriocarcinoma, cutaneous basocellular carcinoma, and testicular seminoma, and Kaposi&#39;s sarcoma. 
     CNS and Neurological Disorders 
     In other embodiments, the disease, condition or disorder is a central nervous system or a neurological disorder. Non-limiting examples of such diseases, conditions or disorders include movement disorders such as tremors, bradykinesias, gait disorders, dystonias, dyskinesias, tardive dyskinesias, other extrapyramidal syndromes, Parkinson&#39;s disease, and disorders associated with Parkinson&#39;s disease. The compounds of the invention also have the potential, or are believed to have the potential, for use in preventing or reducing the effect of drugs that cause or worsen such movement disorders. 
     Infections 
     In other embodiments, the disease, condition or disorder is an infective disorder. Non-limiting examples of such diseases, conditions or disorders include an acute or chronic viral infection, a bacterial infection, a fungal infection, or a parasitic infection. In one embodiment, the viral infection is human immunodeficiency virus. In another embodiment, the viral infection is cytomegalovirus. 
     Immune Disease 
     In other embodiments, the disease, condition or disorder is an immune-related disease, condition or disorder. Non-limiting examples of immune-related diseases, conditions, or disorders include multiple sclerosis and bacterial infections. (See, e.g., Safarzadeh, E. et al., Inflamm Res 2016 65(7):511-20; and Antonioli, L., et al., Immunol Lett S0165-2478(18)30172-X 2018). 
     Additional Indications 
     Other diseases, conditions, and disorders that have the potential to be treated or prevented, in whole or in part, by the inhibition of the A2a and/or A2b adenosine receptor(s) are also candidate indications for the compounds of the invention and salts thereof. Non-limiting examples of other diseases, conditions or disorders in which a compound of the invention, or a pharmaceutically acceptable salt thereof, may be useful include the treatment of hypersensitivity reaction to a tumor antigen and the amelioration of one or more complications related to bone marrow transplant or to a peripheral blood stem cell transplant. Thus, in another embodiment, the present invention provides a method for treating a subject receiving a bone marrow transplant or a peripheral blood stem cell transplant by administering to said subject a therapeutically effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, sufficient to increase the delayed-type hypersensitivity reaction to tumor antigen, to delay the time-to-relapse of post-transplant malignancy, to increase relapse-free survival time post-transplant, and/or to increase long-term post-transplant survival. 
     Combination Therapy 
     In another aspect, the present invention provides methods for the use of a compound of the invention, or a pharmaceutically acceptable salt thereof, (or a pharmaceutically acceptable composition comprising a compound of the invention or pharmaceutically acceptable salt thereof) in combination with one or more additional agents. Such additional agents may have some adenosine A2a and/or A2b receptor activity, or, alternatively, they may function through distinct mechanisms of action. The compounds of the invention may be used in combination with one or more other drugs in the treatment, prevention, suppression or amelioration of diseases or conditions for which the compounds of the invention or the other drugs described herein may have utility, where the combination of the drugs together are safer or more effective than either drug alone. The combination therapy may have an additive or synergistic effect. Such other drug(s) may be administered in an amount commonly used therefore, contemporaneously or sequentially with a compound of the invention or a pharmaceutically acceptable salt thereof. When a compound of the invention is used contemporaneously with one or more other drugs, the pharmaceutical composition may in specific embodiments contain such other drugs and the compound of the invention or its pharmaceutically acceptable salt in separate doses or in unit dosage form. However, the combination therapy may also include therapies in which the compound of the invention or its pharmaceutically acceptable salt and one or more other drugs are administered sequentially, on different or overlapping schedules. It is also contemplated that when used in combination with one or more other active ingredients, the compounds of the invention and the other active ingredients may be used in lower doses than when each is used singly. Accordingly, the pharmaceutical compositions comprising the compounds of the invention include those that contain one or more other active ingredients, in addition to a compound of the invention or a pharmaceutically acceptable salt thereof. 
     The weight ratio of the compound of the present invention to the second active ingredient may be varied and will depend upon the effective dose of each ingredient. Generally, an effective dose of each will be used. Thus, for example, when a compound of the invention is used in combination with another agent, the weight ratio of the compound of the present invention to the other agent may generally range from about 1000:1 to about 1:1000, in particular embodiments from about 200:1 to about 1:200. Combinations of a compound of the present invention and other active ingredients will generally also be within the aforementioned range, but in each case, an effective dose of each active ingredient should generally be used. 
     Given the immunosuppressive role of adenosine, the administration of an A2a receptor antagonist, an A2b receptor antagonist, and/or an A2a/A2b receptor dual antagonist according to the invention may enhance the efficacy of immunotherapies such as PD-1 antagonists. Thus, in one embodiment, the additional therapeutic agent comprises an anti-PD-1 antibody. In another embodiment, the additional therapeutic agent is an anti-PD-L1 antibody. 
     As noted above, PD-1 is recognized as having an important role in immune regulation and the maintenance of peripheral tolerance. PD-1 is moderately expressed on naive T-cells, B-cells and NKT-cells and up-regulated by T-cell and B-cell receptor signaling on lymphocytes, monocytes and myeloid cells (Sharpe et al., Nature Immunology (2007); 8:239-245). 
     Two known ligands for PD-1, PD-L1 (B7-H1) and PD-L2 (B7-DC) are expressed in human cancers arising in various tissues. In large sample sets of, for example, ovarian, renal, colorectal, pancreatic, and liver cancers, and in melanoma, it was shown that PD-L1 expression correlated with poor prognosis and reduced overall survival irrespective of subsequent treatment. (Dong et al., Nat Med. 8(8):793-800 (2002); Yang et al., Invest Ophthamol Vis Sci. 49: 2518-2525 (2008); Ghebeh et al., Neoplasia 8:190-198 (2006); Hamanishi et al., Proc. Natl. Acad. Sci. USA 104: 3360-3365 (2007); Thompson et al., Cancer 5: 206-211 (2006); Nomi et al., Clin. Cancer Research 13:2151-2157 (2007); Ohigashi et al., Clin. Cancer Research 11: 2947-2953; Inman et al., Cancer 109: 1499-1505 (2007); Shimauchi et al., Int. J. Cancer 121:2585-2590 (2007); Gao et al., Clin. Cancer Research 15: 971-979 (2009); Nakanishi J., Cancer Immunol Immunother. 56: 1173-1182 (2007); and Hino et al., Cancer 00: 1-9 (2010)). 
     Similarly, PD-1 expression on tumor infiltrating lymphocytes was found to mark dysfunctional T-cells in breast cancer and melanoma (Ghebeh et al., BMC Cancer. 2008 8:5714-15 (2008); and Ahmadzadeh et al., Blood 114: 1537-1544 (2009)) and to correlate with poor prognosis in renal cancer (Thompson et al., Clinical Cancer Research 15: 1757-1761(2007)). Thus, it has been proposed that PD-L1 expressing tumor cells interact with PD-1 expressing T-cells to attenuate T-cell activation and to evade immune surveillance, thereby contributing to an impaired immune response against the tumor. 
     Immune checkpoint therapies targeting the PD-1 axis have resulted in groundbreaking improvements in clinical response in multiple human cancers (Brahmer, et al., N Engl J Med 2012, 366: 2455-65; Garon et al., N Engl J Med 2015, 372: 2018-28; Hamid et al., N Engl J Med 2013, 369: 134-44; Robert et al., Lancet 2014, 384: 1109-17; Robert et al., N Engl J Med 2015, 372: 2521-32; Robert et al., N Engl J Med 2015, 372: 320-30; Topalian et al., N Engl J Med 2012, 366: 2443-54; Topalian et al., J Clin Oncol 2014, 32: 1020-30; and Wolchok et al., N Engl J Med 2013, 369: 122-33). 
     “PD-1 antagonist” means any chemical compound or biological molecule that blocks binding of PD-L1 expressed on a cancer cell to PD-1 expressed on an immune cell (T-cell, B-cell or NKT cell) and preferably also blocks binding of PD-L2 expressed on a cancer cell to the immune-cell expressed PD-1. Alternative names or synonyms for PD-1 and its ligands include: PDCD1, PD1, CD279 and SLEB2 for PD-1; PDCD1L1, PDL1, B7H1, B7-4, CD274 and B7-H for PD-L1; and PDCD1L2, PDL2, B7-DC, Btdc and CD273 for PD-L2. In any of the treatment methods, medicaments and uses of the present invention in which a human individual is being treated, the PD-1 antagonist blocks binding of human PD-L1 to human PD-1, and preferably blocks binding of both human PD-L1 and PD-L2 to human PD-1. Human PD-1 amino acid sequences can be found in NCBI Locus No.: NP 005009. Human PD-L1 and PD-L2 amino acid sequences can be found in NCBI Locus No.: NP_054862 and NP_079515, respectively. 
     PD-1 antagonists useful in any of the treatment methods, medicaments and uses of the present invention include a monoclonal antibody (mAb), or antigen binding fragment thereof, which specifically binds to PD-1 or PD-L1, and preferably specifically binds to human PD-1 or human PD-L1. The mAb may be a human antibody, a humanized antibody or a chimeric antibody, and may include a human constant region. In some embodiments the human constant region is selected from the group consisting of IgG1, IgG2, IgG3 and IgG4 constant regions, and in preferred embodiments, the human constant region is an IgG1 or IgG4 constant region. In some embodiments, the antigen binding fragment is selected from the group consisting of Fab, Fab′-SH, F(ab′)2, scFv and Fv fragments. Examples of PD-1 antagonists include, but are not limited to, pembrolizumab (KEYTRUDA®, Merck and Co., Inc., Kenilworth, N.J., USA). “Pembrolizumab” (formerly known as MK-3475, SCH 900475 and lambrolizumab and sometimes referred to as “pembro”) is a humanized IgG4 mAb with the structure described in WHO Drug Information, Vol. 27, No. 2, pages 161-162 (2013). Additional examples of PD-1 antagonists include nivolumab (OPDIVO®, Bristol-Myers Squibb Company, Princeton, N.J., USA), atezolizumab (MPDL3280A; TECENTRIQ®, Genentech, San Francisco, Calif., USA), durvalumab (IMFINZI®, Astra Zeneca Pharmaceuticals, LP, Wilmington, Del., and avelumab (BAVENCIO®, Merck KGaA, Darmstadt, Germany and Pfizer, Inc., New York, N.Y.). 
     Examples of monoclonal antibodies (mAbs) that bind to human PD-1, and useful in the treatment methods, medicaments and uses of the present invention, are described in U.S. Pat. Nos. 7,488,802, 7,521,051, 8,008,449, 8,354,509, 8,168,757, WO2004/004771, WO2004/072286, WO2004/056875, and US2011/0271358. 
     Examples of mAbs that bind to human PD-L1, and useful in the treatment methods, medicaments and uses of the present invention, are described in WO2013/019906, WO2010/077634 A1 and U.S. Pat. No. 8,383,796. Specific anti-human PD-L1 mAbs useful as the PD-1 antagonist in the treatment method, medicaments and uses of the present invention include MPDL3280A, BMS-936559, MEDI4736, MSB0010718C and an antibody which comprises the heavy chain and light chain variable regions of SEQ ID NO:24 and SEQ ID NO:21, respectively, of WO2013/019906. 
     Other PD-1 antagonists useful in any of the treatment methods, medicaments and uses of the present invention include an immunoadhesin that specifically binds to PD-1 or PD-L1, and preferably specifically binds to human PD-1 or human PD-L1, e.g., a fusion protein containing the extracellular or PD-1 binding portion of PD-L1 or PD-L2 fused to a constant region such as an Fc region of an immunoglobulin molecule. Examples of immunoadhesin molecules that specifically bind to PD-1 are described in WO2010/027827 and WO2011/066342. Specific fusion proteins useful as the PD-1 antagonist in the treatment methods, medicaments and uses of the present invention include AMP-224 (also known as B7-DCIg), which is a PD-L2-FC fusion protein that binds to human PD-1. 
     Thus, one embodiment provides for a method of treating cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, in combination with a PD-1 antagonist to a subject in need thereof. In such embodiments, the compounds of the invention, or a pharmaceutically acceptable salt thereof, and PD-1 antagonist are administered concurrently or sequentially. 
     Specific non-limiting examples of such cancers in accordance with this embodiment include melanoma (including unresectable or metastatic melanoma), head &amp; neck cancer (including recurrent or metastatic head and neck squamous cell cancer (HNSCC)), classical Hodgkin lymphoma (cHL), urothelial carcinoma, gastric cancer, cervical cancer, primary mediastinal large-B-cell lymphoma, microsatellite instability-high (MSI-H) cancer, non-small cell lung cancer, hepatocellular carcinoma, clear cell kidney cancer, colorectal cancer, breast cancer, squamous cell lung cancer, basal carcinoma, sarcoma, bladder cancer, endometrial cancer, pancreatic cancer, liver cancer, gastrointestinal cancer, multiple myeloma, renal cancer, mesothelioma, ovarian cancer, anal cancer, biliary tract cancer, esophageal cancer, and salivary cancer. 
     In one embodiment, there is provided a method of treating cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist, wherein said cancer is selected from unresectable or metastatic melanoma, recurrent or metastatic head and neck squamous cell cancer (HNSCC), classical Hodgkin lymphoma (cHL), urothelial carcinoma, gastric cancer, cervical cancer, primary mediastinal large-B-cell lymphoma, microsatellite instability-high (MSI-H) cancer, non-small cell lung cancer, and hepatocellular carcinoma. In one such embodiment, the agent is a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     Pembrolizumab is approved by the U.S. FDA for the treatment of patients with unresectable or metastatic melanoma and for the treatment of certain patients with recurrent or metastatic head and neck squamous cell cancer (HNSCC), classical Hodgkin lymphoma (cHL), urothelial carcinoma, gastric cancer, cervical cancer, primary mediastinal large-B-cell lymphoma, microsatellite instability-high (MSI-H) cancer, non-small cell lung cancer, and hepatocellular carcinoma, as described in the Prescribing Information for KEYTRUDA™ (Merck &amp; Co., Inc., Whitehouse Station, N.J. USA; initial U.S. approval 2014, updated November 2018). In another embodiment, there is provided a method of treating cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with pembrolizumab, wherein said cancer is selected from unresectable or metastatic melanoma, recurrent or metastatic head and neck squamous cell cancer (HNSCC), classical Hodgkin lymphoma (cHL), urothelial carcinoma, gastric cancer, cervical cancer, primary mediastinal large-B-cell lymphoma, microsatellite instability-high (MSI-H) cancer, non-small cell lung cancer, and hepatocellular carcinoma. 
     In another embodiment, there is provided a method of treating cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist, wherein said cancer is selected from melanoma, non-small cell lung cancer, head and neck squamous cell cancer (HNSCC), Hodgkin lymphoma, primary mediastinal large B-cell lymphoma, urothelial carcinoma, micro satellite instability-high cancer, gastric cancer, Merkel cell carcinoma, hepatocellular carcinoma, esophageal cancer and cervical cancer. In one such embodiment, the agent is a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. In another such embodiment, the agent is durvalumab. In another such embodiment, the agent is avelumab. 
     In another embodiment, there is provided a method of treating cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist, wherein said cancer is selected from melanoma, non-small cell lung cancer, small cell lung cancer, head and neck cancer, bladder cancer, breast cancer, gastrointestinal cancer, multiple myeloma, hepatocellular cancer, lymphoma, renal cancer, mesothelioma, ovarian cancer, esophageal cancer, anal cancer, biliary tract cancer, colorectal cancer, cervical cancer, thyroid cancer, and salivary cancer. In one such embodiment, the agent is a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. In another such embodiment, the agent is durvalumab. In another such embodiment, the agent is avelumab. 
     In one embodiment, there is provided a method of treating unresectable or metastatic melanoma comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     In one embodiment, there is provided a method of treating recurrent or metastatic head and neck squamous cell cancer (HNSCC) comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     In one embodiment, there is provided a method of treating classical Hodgkin lymphoma (cHL) comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     In one embodiment, there is provided a method of treating urothelial carcinoma comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     In one embodiment, there is provided a method of treating gastric cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     In one embodiment, there is provided a method of treating cervical cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     In one embodiment, there is provided a method of treating primary mediastinal large-B-cell lymphoma comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     In one embodiment, there is provided a method of treating microsatellite instability-high (MSI-H) cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     In one embodiment, there is provided a method of treating non-small cell lung cancer comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     In one embodiment, there is provided a method of treating hepatocellular carcinoma comprising administering an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, to a person in need thereof, in combination with a PD-1 antagonist. In one such embodiment, the agent is pembrolizumab. In another such embodiment, the agent is nivolumab. In another such embodiment, the agent is atezolizumab. 
     In another embodiment, the additional therapeutic agent is at least one immunomodulator other than an A2a or A2b receptor inhibitor. Non-limiting examples of immunomodulators include CD40L, B7, B7RP1, anti-CD40, anti-CD38, anti-ICOS, 4-IBB ligand, dendritic cell cancer vaccine, IL2, IL12, ELC/CCL19, SLC/CCL21, MCP-1, IL-4, IL-18, TNF, IL-15, MDC, IFN-a/-13, M-CSF, IL-3, GM-CSF, IL-13, anti-IL-10 and indolamine 2,3-dioxygenase 1 (ID01) inhibitors. 
     In another embodiment, the additional therapeutic agent comprises radiation. Such radiation includes localized radiation therapy and total body radiation therapy. 
     In another embodiment, the additional therapeutic agent is at least one chemotherapeutic agent. Non-limiting examples of chemotherapeutic agents contemplated for use in combination with the compounds of the invention include: pemetrexed, alkylating agents (e.g., nitrogen mustards such as chlorambucil, cyclophosphamide, isofamide, mechlorethamine, melphalan, and uracil mustard; aziridines such as thiotepa; methanesulphonate esters such as busulfan; nucleoside analogs (e.g., gemcitabine); nitroso ureas such as carmustine, lomustine, and streptozocin; topoisomerase 1 inhibitors (e.g., irinotecan); platinum complexes such as cisplatin, carboplatin and oxaliplatin; bioreductive alkylators such as mitomycin, procarbazine, dacarbazine and altretamine); anthracycline-based therapies (e.g., doxorubicin, daunorubicin, epirubicin and idarubicin); DNA strand-breakage agents (e.g., bleomycin); topoisomerase II inhibitors (e.g., amsacrine, dactinomycin, daunorubicin, idarubicin, mitoxantrone, doxorubicin, etoposide, and teniposide); DNA minor groove binding agents (e.g., plicamydin); antimetabolites (e.g., folate antagonists such as methotrexate and trimetrexate; pyrimidine antagonists such as fluorouracil, fluorodeoxyuridine, CB3717, azacitidine, cytarabine, and floxuridine; purine antagonists such as mercaptopurine, 6-thioguanine, fludarabine, pentostatin; asparginase; and ribonucleotide reductase inhibitors such as hydroxyurea); tubulin interactive agents (e.g., vincristine, estramustine, vinblastine, docetaxol, epothilone derivatives, and paclitaxel); hormonal agents (e.g., estrogens; conjugated estrogens; ethynyl estradiol; diethylstilbesterol; chlortrianisen; idenestrol; progestins such as hydroxyprogesterone caproate, medroxyprogesterone, and megestrol; and androgens such as testosterone, testosterone propionate, fluoxymesterone, and methyltestosterone); adrenal corticosteroids (e.g., prednisone, dexamethasone, methylprednisolone, and prednisolone); luteinizing hormone releasing agents or gonadotropin-releasing hormone antagonists (e.g., leuprolide acetate and goserelin acetate); and antihormonal antigens (e.g., tamoxifen, antiandrogen agents such as flutamide; and antiadrenal agents such as mitotane and aminoglutethimide). 
     In another embodiment, the additional therapeutic agent is at least one signal transduction inhibitor (STI). Non-limiting examples of signal transduction inhibitors include BCR/ABL kinase inhibitors, epidermal growth factor (EGF) receptor inhibitors, HER-2/neu receptor inhibitors, and farnesyl transferase inhibitors (FTIs). 
     In another embodiment, the additional therapeutic agent is at least one anti-infective agent. Non-limiting examples of anti-infective agents include cytokines, non-limiting examples of which include granulocyte-macrophage colony stimulating factor (GM-CSF) and an flt3-ligand. 
     In another embodiment, the present invention provides a method for treating or preventing a viral infection (e.g., a chronic viral infection) including, but not limited to, hepatitis C virus (HCV), human papilloma virus (HPV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), varicella zoster virus, coxsackievirus, and human immunodeficiency virus (HIV). 
     In another embodiment, the present invention provides a method for the treatment of an infective disorder, said method comprising administering to a subject in need thereof an effective amount of a compound of the invention, or a pharmaceutically acceptable salt thereof, in combination with a vaccine. In some embodiments, the vaccine is an anti-viral vaccine, including, for example, an anti-HTV vaccine. Other antiviral agents contemplated for use include an anti-HIV, anti-HPV, anti HCV, anti HSV agents and the like. In other embodiments, the vaccine is effective against tuberculosis or malaria. In still other embodiments, the vaccine is a tumor vaccine (e.g., a vaccine effective against melanoma); the tumor vaccine may comprise genetically modified tumor cells or a genetically modified cell line, including genetically modified tumor cells or a genetically modified cell line that has been transfected to express granulocyte-macrophage stimulating factor (GM-CSF). In another embodiment, the vaccine includes one or more immunogenic peptides and/or dendritic cells. 
     In another embodiment, the present invention provides for the treatment of an infection by administering a compound of the invention, or a pharmaceutically acceptable salt thereof, and at least one additional therapeutic agent, wherein a symptom of the infection observed after administering both the compound of the invention (or a pharmaceutically acceptable salt thereof) and the additional therapeutic agent is improved over the same symptom of infection observed after administering either alone. In some embodiments, the symptom of infection observed can be reduction in viral load, increase in CD4+ T cell count, decrease in opportunistic infections, increased survival time, eradication of chronic infection, or a combination thereof. 
     Definitions 
     As used herein, unless otherwise specified, the following terms have the following meanings. 
     Unsatisfied valences in the text, schemes, examples, structural formulae, and any Tables herein are assumed to have a hydrogen atom or atoms of sufficient number to satisfy the valences. 
     When a variable appears more than once in any moiety or in any compound of the invention (e.g., aryl, heterocycle, N(R) 2 ), the selection of moieties defining that variable for each occurrence is independent of its definition at every other occurrence unless specified otherwise in the local variable definition. 
     As used herein, unless otherwise specified, the term “A2a receptor antagonist” (equivalently, A2a antagonist) and/or “A2b receptor antagonist” (equivalently, A2b antagonist) means a compound exhibiting a potency (IC 50 ) of less than about 1 μM with respect to the A2a and/or A2b receptors, respectively, when assayed in accordance with the procedures described herein. Preferred compounds exhibit at least 10-fold selectivity for antagonizing the A2a receptor and/or the A2b receptor over any other adenosine receptor (e.g., A1 or A3). 
     As described herein, unless otherwise indicated, the use of a compound in treatment means that an amount of the compound, generally presented as a component of a formulation that comprises other excipients, is administered in aliquots of an amount, and at time intervals, which provides and maintains at least a therapeutic serum level of at least one pharmaceutically active form of the compound over the time interval between dose administrations. 
     The phrase “at least one” used in reference to the number of components comprising a composition, for example, “at least one pharmaceutical excipient” means that one member of the specified group is present in the composition, and more than one may additionally be present. Components of a composition are typically aliquots of isolated pure material added to the composition, where the purity level of the isolated material added into the composition is the normally accepted purity level for a reagent of the type. 
     Whether used in reference to a substituent on a compound or a component of a pharmaceutical composition the phrase “one or more”, means the same as “at least one”. 
     “Concurrently” and “contemporaneously” both include in their meaning (1) simultaneously in time (e.g., at the same time); and (2) at different times but within the course of a common treatment schedule. 
     “Consecutively” means one following the other. 
     “Sequentially” refers to a series administration of therapeutic agents that awaits a period of efficacy to transpire between administering each additional agent; this is to say that after administration of one component, the next component is administered after an effective time period after the first component; the effective time period is the amount of time given for realization of a benefit from the administration of the first component. 
     “Effective amount” or “therapeutically effective amount” is meant to describe the provision of an amount of at least one compound of the invention or of a composition comprising at least one compound of the invention which is effective in treating or inhibiting a disease or condition described herein, and thus produce the desired therapeutic, ameliorative, inhibitory or preventative effect. For example, in treating a cancer as described herein with one or more of the compounds of the invention optionally in combination with one or more additional agents, “effective amount” (or “therapeutically effective amount”) means, for example, providing the amount of at least one compound of the invention that results in a therapeutic response in a patient afflicted with the disease, condition, or disorder, including a response suitable to manage, alleviate, ameliorate, or treat the condition or alleviate, ameliorate, reduce, or eradicate one or more symptoms attributed to the condition and/or long-term stabilization of the condition, for example, as may be determined by the analysis of pharmacodynamic markers or clinical evaluation of patients afflicted with the condition. 
     “Patient” and “subject” means an animal, such as a mammal (e.g., a human being) and is preferably a human being. 
     “Prodrug” means compounds that are rapidly transformed, for example, by hydrolysis in blood, in vivo to the parent compound, e.g., conversion of a prodrug of a compound of the invention to a compound of the invention, or to a salt thereof. A thorough discussion is provided in T. Higuchi and V. Stella, Pro-drugs as Novel Delivery Systems, Vol. 14 of the A.C.S. Symposium Series, and in Edward B. Roche, ed., Bioreversible Carriers in Drug Design, American Pharmaceutical Association and Pergamon Press, 1987, both of which are incorporated herein by reference; the scope of this invention includes prodrugs of the novel compounds of this invention. 
     The term “substituted” means that one or more of the moieties enumerated as substituents (or, where a list of substituents are not specifically enumerated, the substituents specified elsewhere in this application) for the particular type of substrate to which said substituent is appended, provided that such substitution does not exceed the normal valence rules for the atom in the bonding configuration presented in the substrate, and that the substitution ultimate provides a stable compound, which is to say that such substitution does not provide compounds with mutually reactive substituents located geminal or vicinal to each other; and wherein the substitution provides a compound sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture. 
     Where optional substitution by a moiety is described (e.g. “optionally substituted”) the term means that if substituents are present, one or more of the enumerated (or default) moieties listed as optional substituents for the specified substrate can be present on the substrate in a bonding position normally occupied by the default substituent, for example, a hydrogen atom on an alkyl chain can be substituted by one of the optional substituents, in accordance with the definition of “substituted” presented herein. 
     “Alkyl” means an aliphatic hydrocarbon group, which may be straight or branched, comprising 1 to 10 carbon atoms. “(C 1 -C 6 )alkyl” means an aliphatic hydrocarbon group, which may be straight or branched, comprising 1 to 6 carbon atoms. Branched means that one or more lower alkyl groups such as methyl, ethyl or propyl, are attached to a linear alkyl chain. Non-limiting examples of alkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, i-butyl, and t-butyl. 
     “Haloalkyl” means an alkyl as defined above wherein one or more hydrogen atoms on the alkyl (up to and including each available hydrogen group) is replaced by a halogen atom. As appreciated by those of skill in the art, “halo” or “halogen” as used herein is intended to include chloro (Cl), fluoro (F), bromo (Br) and iodo (I). Chloro (Cl) and fluoro(F) halogens are generally preferred. 
     “Aryl” means an aromatic monocyclic or multicyclic ring system comprising 6 to 14 carbon atoms, preferably 6 to 10 carbon atoms. The aryl group can be optionally substituted with one or more “ring system substituents” which may be the same or different, and are as defined herein. Non-limiting examples of suitable aryl groups include phenyl and naphthyl. “Monocyclic aryl” means phenyl. 
     “Heteroaryl” means an aromatic monocyclic or multicyclic ring system comprising 5 to 14 ring atoms, preferably 5 to 10 ring atoms, in which one or more of the ring atoms is an element other than carbon, for example nitrogen, oxygen or sulfur, alone or in combination. Preferred heteroaryls contain 5 to 6 ring atoms. The “heteroaryl” can be optionally substituted by one or more substituents, which may be the same or different, as defined herein. The prefix aza, oxa or thia before the heteroaryl root name means that at least a nitrogen, oxygen or sulfur atom respectively, is present as a ring atom. A nitrogen atom of a heteroaryl can be optionally oxidized to the corresponding N-oxide. “Heteroaryl” may also include a heteroaryl as defined above fused to an aryl as defined above. Non-limiting examples of suitable heteroaryls include pyridyl, pyrazinyl, furanyl, thienyl (which alternatively may be referred to as thiophenyl), pyrimidinyl, pyridone (including N-substituted pyridones), isoxazolyl, isothiazolyl, oxazolyl, oxadiazolyl, thiazolyl, thiadiazolyl, pyrazolyl, furazanyl, pyrrolyl, pyrazolyl, triazolyl, 1,2,4-thiadiazolyl, pyrazinyl, pyridazinyl, quinoxalinyl, phthalazinyl, oxindolyl, imidazo[1,2-a]pyridinyl, imidazo[2,1-b]thiazolyl, benzofurazanyl, indolyl, azaindolyl, benzimidazolyl, benzothienyl, quinolinyl, imidazolyl, thienopyridyl, quinazolinyl, thienopyrimidyl, pyrrolopyridyl, imidazopyridyl, isoquinolinyl, benzoazaindolyl, 1,2,4-triazinyl, benzothiazolyl and the like. The term “heteroaryl” also refers to partially saturated heteroaryl moieties such as, for example, tetrahydroisoquinolyl, tetrahydroquinolyl and the like. The term “monocyclic heteroaryl” refers to monocyclic versions of heteroaryl as described above and includes 4- to 7-membered monocyclic heteroaryl groups comprising from 1 to 4 ring heteroatoms, said ring heteroatoms being independently selected from the group consisting of N, O, and S, and oxides thereof. The point of attachment to the parent moiety is to any available ring carbon or ring heteroatom. Non-limiting examples of monocyclic heteroaryl moieties include pyridyl, pyrazinyl, furanyl, thienyl, pyrimidinyl, pyridazinyl, pyridinyl, thiazolyl, isothiazolyl, oxazolyl, oxadiazolyl, isoxazolyl, pyrazolyl, furazanyl, pyrrolyl, pyrazolyl, triazolyl, thiadiazolyl (e.g., 1,2,4-thiadiazolyl), imidazolyl, and triazinyl (e.g., 1,2,4-triazinyl), and oxides thereof. 
     “Cycloalkyl” means a non-aromatic fully saturated monocyclic or multicyclic ring system comprising 3 to 10 carbon atoms, preferably 3 to 6 carbon atoms. The cycloalkyl can be optionally substituted with one or more substituents, which may be the same or different, as described herein. Monocyclic cycloalkyl refers to monocyclic versions of the cycloalkyl moieties described herein. Non-limiting examples of suitable monocyclic cycloalkyls include cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl and the like. Non-limiting examples of multicyclic cycloalkyls include [1.1.1]-bicyclopentane, 1-decalinyl, norbornyl, adamantyl and the like. 
     “Heterocycloalkyl” (or “heterocyclyl”) means a non-aromatic saturated monocyclic or multicyclic ring system comprising 3 to 10 ring atoms, preferably 5 to 10 ring atoms, in which one or more of the atoms in the ring system is an element other than carbon, for example nitrogen, oxygen or sulfur, alone or in combination. There are no adjacent oxygen and/or sulfur atoms present in the ring system. Preferred heterocycloalkyl groups contain 4, 5 or 6 ring atoms. The prefix aza, oxa or thia before the heterocyclyl root name means that at least a nitrogen, oxygen or sulfur atom respectively is present as a ring atom. Any —NH in a heterocyclyl ring may exist protected such as, for example, as an —N(Boc), —N(CBz), —N(Tos) group and the like; such protections are also considered part of this invention. The heterocyclyl can be optionally substituted by one or more substituents, which may be the same or different, as described herein. The nitrogen or sulfur atom of the heterocyclyl can be optionally oxidized to the corresponding N-oxide, S-oxide or S,S-dioxide. Thus, the term “oxide,” when it appears in a definition of a variable in a general structure described herein, refers to the corresponding N-oxide, S-oxide, or S,S-dioxide. “Heterocyclyl” also includes rings wherein ═O replaces two available hydrogens on the same carbon atom (i.e., heterocyclyl includes rings having a carbonyl group in the ring). Such ═O groups may be referred to herein as “oxo.” An example of such a moiety is pyrrolidinone (or pyrrolidone): 
     
       
         
         
             
             
         
       
     
     As used herein, the term “monocyclic heterocycloalkyl” refers to monocyclic versions of the heterocycloalkyl moieties described herein and include a 4- to 7-membered monocyclic heterocycloalkyl groups comprising from 1 to 4 ring heteroatoms, said ring heteroatoms being independently selected from the group consisting of N, N-oxide, O, S, S-oxide, S(O), and S(O) 2 . The point of attachment to the parent moiety is to any available ring carbon or ring heteroatom. Non-limiting examples of monocyclic heterocycloalkyl groups include piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof. Non-limiting examples of lower alkyl-substituted oxetanyl include the moiety: 
     
       
         
         
             
             
         
       
     
     It is noted that in hetero-atom containing ring systems of this invention, there are no hydroxyl groups on carbon atoms adjacent to a N, O or S, and there are no N or S groups on carbon adjacent to another heteroatom. 
     
       
         
         
             
             
         
       
     
     there is no —OH attached directly to carbons marked 2 and 5. 
     The line  , as a bond generally indicates a mixture of, or either of, the possible isomers, e.g., containing (R)- and (S)-stereochemistry. For example: 
     
       
         
         
             
             
         
       
     
     means containing both 
     
       
         
         
             
             
         
       
     
     The wavy line  , as used herein, indicates a point of attachment to the rest of the compound. Lines drawn into the ring systems, such as, for example: 
     
       
         
         
             
             
         
       
     
     indicate that the indicated line (bond) may be attached to any of the substitutable ring atoms. 
     “Oxo” is defined as an oxygen atom that is double bonded to a ring carbon in a cycloalkyl, cycloalkenyl, heterocyclyl, heterocyclenyl, or other ring described herein, e.g., 
     
       
         
         
             
             
         
       
     
     As well known in the art, a bond drawn from a particular atom wherein no moiety is depicted at the terminal end of the bond indicates a methyl group bound through that bond to the atom, unless stated otherwise. For example: 
     
       
         
         
             
             
         
       
     
     represents 
     
       
         
         
             
             
         
       
     
     One or more compounds of the invention may also exist as, or optionally be converted to, a solvate. Preparation of solvates is generally known. Thus, for example, M. Caira et al., J. Pharmaceutical Sci., 93(3), 601-611 (2004) describe the preparation of the solvates of the antifungal fluconazole in ethyl acetate as well as from water. Similar preparations of solvates, and hemisolvate, including hydrates (where the solvent is water or aqueous-based) and the like are described by E. C. van Tonder et al., AAPS PharmSciTech., 5(1), article 12 (2004); and A. L. Bingham et al., Chem. Commun., 603-604 (2001). A typical, non-limiting, process involves dissolving the inventive compound in desired amounts of the desired solvent (for example, an organic solvent, an aqueous solvent, water or mixtures of two or more thereof) at a higher than ambient temperature, and cooling the solution, with or without an antisolvent present, at a rate sufficient to form crystals which are then isolated by standard methods. Analytical techniques such as, for example I.R. spectroscopy, show the presence of the solvent (including water) in the crystals as a solvate (or hydrate in the case where water is incorporated into the crystalline form). 
     The term “purified”, “in purified form” or “in isolated and purified form” for a compound refers to the physical state of said compound after being isolated from a synthetic process or natural source or combination thereof. Thus, the term “purified”, “in purified form” or “in isolated and purified form” for a compound refers to the physical state of said compound after being obtained from a purification process or processes described herein or well known to the skilled artisan, and in sufficient purity to be characterized by standard analytical techniques described herein or well known to the skilled artisan. 
     This invention also includes the compounds of the invention in isolated and purified form obtained by routine techniques. Polymorphic forms of the compounds of the invention, and of the salts, solvates and prodrugs of the thereof, are intended to be included in the present invention. Certain compounds of the invention may exist in different isomeric forms (e.g., enantiomers, diastereoisomers, atropisomers). The inventive compounds include all isomeric forms thereof, both in pure form and admixtures of two or more, including racemic mixtures. 
     In similar manner, unless indicated otherwise, presenting a structural representation of any tautomeric form of a compound which exhibits tautomerism is meant to include all such tautomeric forms of the compound. Accordingly, where compounds of the invention, their salts, and solvates and prodrugs thereof, may exist in different tautomeric forms or in equilibrium among such forms, all such forms of the compound are embraced by, and included within the scope of the invention. Examples of such tautomers include, but are not limited to, ketone/enol tautomeric forms, imine-enamine tautomeric forms, and for example heteroaromatic forms such as the following moieties: 
     
       
         
         
             
             
         
       
     
     Where a reaction scheme appearing in an example employs a compound having one or more stereocenters, the stereocenters are indicated with an asterisk, as shown below: 
     
       
         
         
             
             
         
       
     
     Accordingly, the above depiction consists of the following pairs of isomers: (i) Trans-isomers ((2R,7aS)-2-methylhexahydro-1H-pyrrolizin-7a-yl)methanamine (Compound ABC-1) and ((2S,7aR)-2-methylhexahydro-1H-pyrrolizin-7a-yl)methanamine (Compound ABC-2); and (ii) Cis-isomers ((2R,7aR)-2-methylhexahydro-1H-pyrrolizin-7a-yl)methanamine (Compound ABC-3) and ((2S,7aS)-2-methylhexahydro-1H-pyrrolizin-7a-yl)methanamine (Compound ABC-4). 
     
       
         
         
             
             
         
       
     
     All stereoisomers of the compounds of the invention (including salts and solvates of the inventive compounds and their prodrugs), such as those which may exist due to asymmetric carbons present in a compound of the invention, and including enantiomeric forms (which may exist even in the absence of asymmetric carbons), rotameric forms, atropisomers, and diastereomeric forms, are contemplated within the scope of this invention. Individual stereoisomers of the compounds of the invention may be isolated in a pure form, for example, substantially free of other isomers, or may be isolated as an admixture of two or more stereoisomers or as a racemate. The chiral centers of the present invention can have the S or R configuration as defined by the IUPAC 1974 Recommendations. The use of the terms “salt”, “solvate” “prodrug” and the like, is intended to equally apply to salts, solvates and prodrugs of isolated enantiomers, stereoisomer pairs or groups, rotamers, tautomers, or racemates of the inventive compounds. 
     Where diastereomeric mixtures can be separated into their individual diastereomers on the basis of their physical chemical differences by known methods, for example, by chiral chromatography and/or fractional crystallization, simple structural representation of the compound contemplates all diastereomers of the compound. As is known, enantiomers may also be separated by converting the enantiomeric mixture into a diastereomeric mixture by reaction with an appropriate optically active compound (e.g., chiral auxiliary such as a chiral alcohol or Mosher&#39;s acid chloride), separating the diastereomers and converting (e.g., hydrolyzing) the individually isolated diastereomers to the corresponding purified enantiomers. 
     As the term is employed herein, salts of the inventive compounds, whether acidic salts formed with inorganic and/or organic acids, basic salts formed with inorganic and/or organic bases, salts formed which include zwitterionic character, for example, where a compound contains both a basic moiety, for example, but not limited to, a nitrogen atom, for example, an amine, pyridine or imidazole, and an acidic moiety, for example, but not limited to a carboxylic acid, are included in the scope of the inventive compounds described herein. The formation of pharmaceutically useful salts from basic (or acidic) pharmaceutical compounds are discussed, for example, by S. Berge et al., Journal of Pharmaceutical Sciences (1977) 66(1) 1-19; P. Gould, International J. of Pharmaceutics (1986) 33 201-217; Anderson et al., The Practice of Medicinal Chemistry (1996), Academic Press, New York; in The Orange Book (Food &amp; Drug Administration, Washington, D.C. on their website); and P. Heinrich Stahl, Camille G. Wermuth (Eds.), Handbook of Pharmaceutical Salts: Properties, Selection, and Use, (2002) Int&#39;l. Union of Pure and Applied Chemistry, pp. 330-331. These disclosures are incorporated herein by reference. 
     The present invention contemplates all available salts, including salts which are generally recognized as safe for use in preparing pharmaceutical formulations and those which may be formed presently within the ordinary skill in the art and are later classified as being “generally recognized as safe” for use in the preparation of pharmaceutical formulations, termed herein as “pharmaceutically acceptable salts”. Examples of pharmaceutically acceptable acid addition salts include, but are not limited to, acetates, including trifluoroacetate salts, adipates, alginates, ascorbates, aspartates, benzoates, benzenesulfonates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, cyclopentanepropionates, digluconates, dodecylsulfates, ethanesulfonates, fumarates, glucoheptanoates, glycerophosphates, hemisulfates, heptanoates, hexanoates, hydrochlorides, hydrobromides, hydroiodides, 2-hydroxyethanesulfonates, lactates, maleates, methanesulfonates, methyl sulfates, 2-naphthalenesulfonates, nicotinates, nitrates, oxalates, pamoates, pectinates, persulfates, 3-phenylpropionates, phosphates, picrates, pivalates, propionates, salicylates, succinates, sulfates, sulfonates (such as those mentioned herein), tartarates, thiocyanates, toluenesulfonates (also known as tosylates) undecanoates, and the like. 
     Examples of pharmaceutically acceptable basic salts include, but are not limited to, ammonium salts, alkali metal salts such as sodium, lithium, and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, aluminum salts, zinc salts, salts with organic bases (for example, organic amines) such as benzathines, diethylamine, dicyclohexylamines, hydrabamines (formed with N,N-bis(dehydroabietyl)ethylenediamine), N-methyl-D-glucamines, N-methyl-D-glucamides, t-butyl amines, piperazine, phenylcyclohexyl-amine, choline, tromethamine, and salts with amino acids such as arginine, lysine and the like. Basic nitrogen-containing groups may be converted to an ammonium ion or quarternized with agents such as lower alkyl halides (e.g. methyl, ethyl, propyl, and butyl chlorides, bromides and iodides), dialkyl sulfates (e.g. dimethyl, diethyl, dibutyl, and diamyl sulfates), long chain halides (e.g. decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides), arylalkyl halides (e.g. benzyl and phenethyl bromides), and others. 
     All such acid and base salts are intended to be pharmaceutically acceptable salts within the scope of the invention and all acid and base salts are considered equivalent to the free forms of the corresponding compounds for purposes of the scope of the invention. 
     A functional group in a compound termed “protected” means that the group is in modified form to preclude undesired side reactions at the protected site when the protected compound is subjected to particular reaction conditions aimed at modifying another region of the molecule. Suitable protecting groups are known, for example, as by reference to standard textbooks, for example, T. W. Greene et al., Protective Groups in organic Synthesis (1991), Wiley, New York. 
     In the compounds of the invention, the atoms may exhibit their natural isotopic abundances, or one or more of the atoms may be artificially enriched in a particular isotope having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number predominantly found in nature. The present invention is meant to include all suitable isotopic variations of the compounds of the invention. For example, different isotopic forms of hydrogen (H) include protium ( 1 H) and deuterium ( 2 H). Protium is the predominant hydrogen isotope found in nature. Enriching for deuterium may afford certain therapeutic advantages, such as increasing in vivo half-life or reducing dosage requirements, or may provide a compound useful as a standard for characterization of biological samples. Isotopically-enriched compounds of the invention can be prepared without undue experimentation by conventional techniques well known to those skilled in the art or by processes analogous to those described in the Schemes and Examples herein using appropriate isotopically-enriched reagents and/or intermediates. 
     The present invention also embraces isotopically-labeled compounds of the present invention which are structurally identical to those recited herein, but for the fact that a statistically significant percentage of one or more atoms in that form of the compound are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number of the most abundant isotope usually found in nature, thus altering the naturally occurring abundance of that isotope present in a compound of the invention. Examples of isotopes that can be preferentially incorporated into compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, iodine, fluorine and chlorine, for example, but not limited to:  2 H,  3 H,  11 C,  13 C,  14 C,  13 N,  15 N,  15 O,  17 O,  18 O,  31 P,  32 P,  35 S,  18 F, and  36 Cl,  123 I and  125 I. It will be appreciated that other isotopes also may be incorporated by known means. 
     Certain isotopically-labeled compounds of the invention (e.g., those labeled with  3 H,  11 C and  14 C) are recognized as being particularly useful in compound and/or substrate tissue distribution assays using a variety of known techniques. Tritiated (i.e.,  3 H) and carbon-14 (i.e.,  14 C) isotopes are particularly preferred for their ease of preparation and detection. Further, substitution of a naturally abundant isotope with a heavier isotope, for example, substitution of protium with deuterium (i.e.,  2 H) may afford certain therapeutic advantages resulting from greater metabolic stability (e.g., increased in vivo half-life or reduced dosage requirements) and hence may be preferred in some circumstances. Isotopically labeled compounds of the invention can generally be prepared by following procedures analogous to those disclosed in the reaction Schemes and/or in the Examples herein below, by substituting an appropriate isotopically labeled reagent for a non-isotopically labeled reagent, or by well-known reactions of an appropriately prepared precursor to the compound of the invention which is specifically prepared for such a “labeling” reaction. Such compounds are included also in the present invention. 
     The term “composition” is intended to encompass a product comprising the specified ingredients in the specified amounts, and any product which results, directly or indirectly, from combination of the specified ingredients in the specified amounts. 
     The term “pharmaceutical composition” as used herein encompasses both the bulk composition and individual dosage units comprised of one, or more than one (e.g., two), pharmaceutically active agents such as, for example, a compound of the present invention (optionally together with an additional agent as described herein), along with any pharmaceutically inactive excipients. As will be appreciated by those of ordinary skill in the art, excipients are any constituent which adapts the composition to a particular route of administration or aids the processing of a composition into a dosage form without itself exerting an active pharmaceutical effect. The bulk composition and each individual dosage unit can contain fixed amounts of the aforesaid one, or more than one, pharmaceutically active agents. The bulk composition is material that has not yet been formed into individual dosage units. 
     It will be appreciated that pharmaceutical formulations of the invention may comprise more than one compound of the invention (or a pharmaceutically acceptable salt thereof), for example, the combination of two or three compounds of the invention, each present in such a composition by adding to the formulation the desired amount of the compound in a pharmaceutically acceptably pure form. It will be appreciated also that in formulating compositions of the invention, a composition may comprise, in addition to one or more of compounds of the invention, one or more other agents which also have pharmacological activity, as described herein. 
     While formulations of the invention may be employed in bulk form, it will be appreciated that for most applications the inventive formulations will be incorporated into a dosage form suitable for administration to a patient, each dosage form comprising an amount of the selected formulation which contains an effective amount of one or more compounds of the invention. Examples of suitable dosage forms include, but are not limited to, dosage forms adapted for: (i) oral administration, e.g., a liquid, gel, powder, solid or semi-solid pharmaceutical composition which is loaded into a capsule or pressed into a tablet and may comprise additionally one or more coatings which modify its release properties, for example, coatings which impart delayed release or formulations which have extended release properties; (ii) a dosage form adapted for intramuscular administration (IM), for example, an injectable solution or suspension, and which may be adapted to form a depot having extended release properties; (iii) a dosage form adapted for intravenous administration (IV), for example, a solution or suspension, for example, as an IV solution or a concentrate to be injected into a saline IV bag; (iv) a dosage form adapted for administration through tissues of the oral cavity, for example, a rapidly dissolving tablet, a lozenge, a solution, a gel, a sachets or a needle array suitable for providing intramucosal administration; (v) a dosage form adapted for administration via the mucosa of the nasal or upper respiratory cavity, for example a solution, suspension or emulsion formulation for dispersion in the nose or airway; (vi) a dosage form adapted for transdermal administration, for example, a patch, cream or gel; (vii) a dosage form adapted for intradermal administration, for example, a microneedle array; and (viii) a dosage form adapted for delivery via rectal or vaginal mucosa, for example, a suppository. 
     For preparing pharmaceutical compositions comprising compounds of the invention, generally the compounds of the invention will be combined with one or more pharmaceutically acceptable excipients. These excipients impart to the composition properties which make it easier to handle or process, for example, lubricants or pressing aids in powdered medicaments intended to be tableted, or adapt the formulation to a desired route of administration, for example, excipients which provide a formulation for oral administration, for example, via absorption from the gastrointestinal tract, transdermal or transmucosal administration, for example, via adhesive skin “patch” or buccal administration, or injection, for example, intramuscular or intravenous, routes of administration. These excipients are collectively termed herein “a carrier”. Typically formulations may comprise up to about 95 percent active ingredient, although formulations with greater amounts may be prepared. 
     Pharmaceutical compositions can be solid, semi-solid or liquid. Solid form preparations can be adapted to a variety of modes of administration, examples of which include, but are not limited to, powders, dispersible granules, mini-tablets, beads, which can be used, for example, for tableting, encapsulation, or direct administration. Liquid form preparations include, but are not limited to, solutions, suspensions and emulsions which for example, but not exclusively, can be employed in the preparation of formulations intended for parenteral injection, for intranasal administration, or for administration to some other mucosal membrane. Formulations prepared for administration to various mucosal membranes may also include additional components adapting them for such administration, for example, viscosity modifiers. 
     Aerosol preparations, for example, suitable for administration via inhalation or via nasal mucosa, may include solutions and solids in powder form, which may be in combination with a pharmaceutically acceptable propellant, for example, an inert compressed gas, e.g. nitrogen. Also included are solid form preparations which are intended to be converted, shortly before use, to a suspension or a solution, for example, for oral or parenteral administration. Examples of such solid forms include, but are not limited to, freeze dried formulations and liquid formulations adsorbed into a solid absorbent medium. 
     The compounds of the invention may also be deliverable transdermally or transmucosally, for example, from a liquid, suppository, cream, foam, gel, or rapidly dissolving solid form. It will be appreciated that transdermal compositions can take also the form of creams, lotions, aerosols and/or emulsions and can be provided in a unit dosage form which includes a transdermal patch of any know in the art, for example, a patch which incorporates either a matrix comprising the pharmaceutically active compound or a reservoir which comprises a solid or liquid form of the pharmaceutically active compound. 
     Examples of pharmaceutically acceptable carriers and methods of manufacture for various compositions mentioned above may be found in A. Gennaro (ed.), Remington: The Science and Practice of Pharmacy, 20 th  Edition, (2000), Lippincott Williams &amp; Wilkins, Baltimore, Md. 
     Preferably, the pharmaceutical preparation is in a unit dosage form. In such form, the preparations subdivided into suitably sized unit doses containing appropriate quantities of the active component, e.g., an effective amount to achieve the desired purpose. 
     The actual dosage employed may be varied depending upon the requirements of the patient and the severity of the condition being treated. Determination of the proper dosage regimen for a particular situation is within the skill in the art. For convenience, the total daily dosage may be divided and administered in portions during the day as required. 
     In accordance with the present invention, antagonism of adenosine A2a and/or A2b receptors is accomplished by administering to a patient in need of such therapy an effective amount of one or more compounds of the invention, or a pharmaceutically acceptable salt thereof. 
     In some embodiments it is preferred for the compound to be administered in the form of a pharmaceutical composition comprising the compound of the invention, or a salt thereof, and at least one pharmaceutically acceptable carrier (described herein). It will be appreciated that pharmaceutically formulations of the invention may comprise more than one compound of the invention, or a salt thereof, for example, the combination of two or three compounds of the invention, or, additionally or alternatively, another active agent such as those described herein, each present by adding to the formulation the desired amount of the compound or a salt thereof (or agent, where applicable) which has been isolated in a pharmaceutically acceptably pure form. 
     As mentioned above, administration of a compound of the invention to effect antagonism of A2a and/or A2b receptors is preferably accomplished by incorporating the compound into a pharmaceutical formulation incorporated into a dosage form, for example, one of the above-described dosage forms comprising an effective amount of at least one compound of the invention (e.g., 1, 2 or 3, or 1 or 2, or 1, and usually 1 compound of the invention), or a pharmaceutically acceptable salt thereof. Methods for determining safe and effective administration of compounds which are pharmaceutically active, for example, a compound of the invention, are known to those skilled in the art, for example, as described in the standard literature, for example, as described in the “Physicians&#39; Desk Reference” (PDR), e.g., 1996 edition (Medical Economics Company, Montvale, N.J. 07645-1742, USA), the Physician&#39;s Desk Reference, 56 th  Edition, 2002 (published by Medical Economics company, Inc. Montvale, N.J. 07645-1742), or the Physician&#39;s Desk Reference, 57 th  Edition, 2003 (published by Thompson PDR, Montvale, N.J. 07645-1742); the disclosures of which is incorporated herein by reference thereto. The amount and frequency of administration of the compounds of the invention and/or the pharmaceutically acceptable salts thereof will be regulated according to the judgment of the attending clinician considering such factors as age, condition and size of the patient as well as severity of the symptoms being treated. Compounds of the invention can be administered at a total daily dosage of up to 1,000 mg, which can be administered in one daily dose or can be divided into multiple doses per 24 hour period, for example, two to four doses per day. 
     As those of ordinary skill in the art will appreciate, an appropriate dosage level for a compound (or compounds) of the invention will generally be about 0.01 to 500 mg per kg patient body weight per day which can be administered in single or multiple doses. A suitable dosage level may be about 0.01 to 250 mg/kg per day, about 0.05 to 100 mg/kg per day, or about 0.1 to 50 mg/kg per day. Within this range the dosage may be 0.05 to 0.5, 0.5 to 5 or 5 to 50 mg/kg per day. For oral administration, the compositions may be provided in the form of tablets containing 1.0 to 1000 milligrams of the active ingredient, particularly 1.0, 5.0, 10.0, 15.0, 20.0, 25.0, 50.0, 75.0, 100.0, 150.0, 200.0, 250.0, 300.0, 400.0, 500.0, 600.0, 750.0, 800.0, 900.0, and 1000.0 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated. The compounds may be administered on a regimen of 1 to 4 times per day, or may be administered once or twice per day. 
     Those skilled in the art will appreciate that treatment protocols utilizing at least one compound of the invention can be varied according to the needs of the patient. Thus, compounds of the invention used in the methods of the invention can be administered in variations of the protocols described above. For example, compounds of the invention can be administered discontinuously rather than continuously during a treatment cycle. 
     In general, in whatever form administered, the dosage form administered will contain an amount of at least one compound of the invention, or a salt thereof, which will provide a therapeutically effective serum level of the compound in some form for a suitable period of time such as at least 2 hours, more preferably at least four hours or longer. In general, as is known in the art, dosages of a pharmaceutical composition providing a therapeutically effective serum level of a compound of the invention can be spaced in time to provide serum level meeting or exceeding the minimum therapeutically effective serum level on a continuous basis throughout the period during which treatment is administered. As will be appreciated the dosage form administered may also be in a form providing an extended release period for the pharmaceutically active compound which will provide a therapeutic serum level for a longer period, necessitating less frequent dosage intervals. As mentioned above, a composition of the invention can incorporate additional pharmaceutically active components or be administered simultaneously, contemporaneously, or sequentially with other pharmaceutically active agents as may be additionally needed or desired in the course of providing treatment. As will be appreciated, the dosage form administered may also be in a form providing an extended release period for the pharmaceutically active compound which will provide a therapeutic serum level for a longer period, necessitating less frequent dosage intervals. 
     PREPARATIVE EXAMPLES 
     The compounds of the present invention can be prepared readily according to the following schemes and specific examples, or modifications thereof, using readily available starting materials, reagents and conventional synthetic procedures. In these reactions, it is also possible to make use of variants which are themselves known to those of ordinary skill in this art but are not mentioned in detail. The general procedures for making the compounds claimed in this invention can be readily understood and appreciated by one skilled in the art from viewing the following Schemes and descriptions. 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     One general strategy for the synthesis of compounds of type G1.6 is via the four-step procedure shown in General Scheme 1, wherein R 4  corresponds to ring A in Formula (I) and wherein R 1 , R 2 , and ring A are as defined in Formula (I). In the first step, amino benzonitriles G1.1 can be treated with 1-(isocyanatomethyl)-2,4-dimethoxybenzene in solvents such as the combination of dichloromethane and pyridine to form intermediate ureas G1.2. In the second step, these ureas can be dehydrated to the corresponding carbodiimides G1.3 in the presence of triphenylphosphine, carbon tetrabromide, and triethylamine in a solvent such as dichloromethane. In the third step, treatment of carbodiimides G1.3 with a hydrazide of the type G1.4 in the presence of acetic acid in a solvent such as dichloromethane or dioxane, produces products of the type G1.5. In the fourth step, the 2,4-dimethoxybenzyl group of G1.4 is removed under acidic conditions to provide products of type G1.6, which can be purified by silica gel chromatography, preparative reversed phase HPLC, and/or chiral SFC. 
     
       
         
         
             
             
         
       
     
     One general strategy for the synthesis of compounds of type G2.5 is via the three-step procedure shown in General Scheme 2, wherein R A (n) corresponds to R A1 , R A2 , R A3 , and R A5  in Formula (I) and wherein R 1 , R 2 , R 3  and R A(n)  (as R A1 , R A2 , R A3 , and R A5 ) are as defined in Formula (I). In the first step, protected cyclic amines G2.1 can be converted into unprotected amines G2.2 through carefully controlled treatment with acid. Acids such as formic acid in the absence of solvent or hydrochloric acid in the presence of MeOH or DCM, can be used. In the second step, intermediates of type G2.2 can be converted into intermediates of type G2.4 through a transition-metal catalyzed C—N coupling reaction with aryl bromides G2.3. The reaction is performed under deoxygenated conditions with palladium catalysts such as, tert-butyl X-Phos Third Generation Precatalyst, a base such as sodium tert-butoxide, and a solvent such as THF, at the appropriate temperature. In the third step, the 2,4-dimethoxybenzyl group of G2.4 is removed under acidic conditions to provide products of type G2.5, which can be purified by silica gel chromatography, preparative reversed-phase HPLC, and/or chiral SFC. 
     
       
         
         
             
             
         
       
     
     One general strategy for the synthesis of compounds of type G3.6 is via a four-step procedure shown in General Scheme 3, wherein R 4  corresponds to ring A in Formula (I) and wherein R 1 , R 2 , and ring A are as defined in Formula (I). In the first step, amino benzoic acids G3.1 can be converted into amino quinazolines G3.2 via treatment with cyanamide in the presence of aqueous HCl in a solvent such as EtOH. In the second step, intermediates of type G3.2 can be converted into intermediates of type G3.3 through coupling with 1,2,4-triazole, following treatment of G3.2 with phosphorous (V) oxychloride in a solvent such as acetonitrile. In the third step, intermediates of type G3.3 can be treated with hydrazides G3.4 in a solvent such as THF, to provide products of type G3.5. In the fourth step, intermediates of type G3.5 can undergo a rearrangement upon heating in neat N,O-Bis(trimethylsilyl)acetamide (BSA) to form products of type G3.6. Products of type G3.6 can be purified by silica gel chromatography, preparative reversed-phase HPLC, and/or chiral SFC. 
     
       
         
         
             
             
         
       
     
     One general strategy for the synthesis of compounds of type G4.5 is via a three-step procedure outlined in General Scheme 4, wherein R 1 , R 2 , R 3 , R A1 , R A2 , and R A3  are defined in Formula (I). Heteroaryl cyclohexanol G4.1 can be converted into the corresponding cyclohexanone G4.2 via oxidation with Dess-Martin periodinane. In the second step, intermediates of type G4.2 can undergo treatment with an R 3 —Li G4.3 at low temperature, to provide products of type G4.4. In the third step, the 2,4-dimethoxybenzyl group of G2.4 can be removed with DDQ to provide products of type G4.5, which can be purified by silica gel chromatography, preparative reversed-phase HPLC, and/or chiral SFC. 
     Experimentals 
     Abbreviations used in the experimentals may include, but are not limited to, the following: 
     
       
         
           
               
               
             
               
                   
               
             
            
               
                 18-crown-6 
                 1,4,7,10,13,16-hexaoxacyclooctadecane 
               
               
                 ° C. 
                 Degrees Celsius 
               
               
                 AcOH 
                 Acetic acid 
               
               
                 aq. 
                 Aqueous 
               
               
                 Atm 
                 Atmospheres 
               
               
                 Boc 2 O 
                 Di-tert-butyl dicarbonate 
               
               
                 BSA 
                 N,O-Bis(trimethylsilyl)acetamide 
               
               
                 CD 3 OD 
                 Deuterated Methanol-d4 
               
               
                 DCM 
                 Dichloromethane 
               
               
                 DDQ 
                 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone 
               
               
                 DEA 
                 Diethylamine 
               
               
                 DIAD 
                 Diisopropyl diazene-1,2-dicarboxylate 
               
               
                 DIBAL 
                 Diisobutylaluminium hydride 
               
               
                 DIPEA 
                 N,N-Diisopropylethylamine 
               
               
                 DMAP 
                 4-(dimethylamino)-pyridine 
               
               
                 DMF 
                 Dimethylformamide 
               
               
                 DMP 
                 Dess-Martin periodinane 
               
               
                 DMSO 
                 Dimethyl Sulfoxide 
               
               
                 DMSO-d6 
                 Deuterated Dimethyl Sulfoxide 
               
               
                 dppf 
                 Bis(diphenylphosphino)ferrocene 
               
               
                 ES 
                 Electrospray Ionization 
               
               
                 Et 2 O 
                 Diethylether 
               
               
                 EtOAc 
                 Ethyl Acetate 
               
               
                 EtOH 
                 Ethanol 
               
               
                 h 
                 Hours 
               
               
                 HPLC 
                 High Performance Liquid Chromatography 
               
               
                 M 
                 Molar 
               
               
                 MeCN 
                 Acetonitrile 
               
               
                 MeOD-d4 
                 Deuterated Methanol 
               
               
                 MeOH 
                 Methanol 
               
               
                 MHz 
                 Megahertz 
               
               
                 min 
                 Minutes 
               
               
                 mL 
                 Milliliters 
               
               
                 MS 
                 Mass Spectroscopy 
               
               
                 MsCl 
                 p-Toluenesulfonyl chloride 
               
               
                 NaH 
                 Sodium hydride 
               
               
                 NBS 
                 N-Bromosuccinimide 
               
               
                 nm 
                 Nanometers 
               
               
                 NMR 
                 Nuclear Magnetic Resonance 
               
               
                 Pd/C 
                 Palladium on Carbon 
               
               
                 PPTS 
                 Pyrdinium para-toluenesulfonate 
               
               
                 p-TsOH 
                 4-Methylbenzenesulfonic acid 
               
               
                 Py 
                 Pyridine 
               
               
                 rac 
                 racemic 
               
               
                 SFC 
                 Supercritical Fluid (CO2) Chromatography 
               
               
                 T3P 
                 Tripropyl phosphonic anhydride 
               
               
                   
               
               
                 tBuXPhos-Pd G3 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Tf 2 O 
                 Trifluoromethanesulfonic anhydride 
               
               
                 TFA 
                 Trifluoroacetic acid 
               
               
                 TFE 
                 2,2,2-Trifluoroethanol 
               
               
                 THF 
                 Tetrahydrofuran 
               
               
                 THP 
                 (tetrahydro-2H-pyran-2-yl)oxy 
               
               
                 TLC 
                 Thin Layer Chromatography 
               
               
                   
               
            
           
         
       
     
     General Experimental Information: 
     Unless otherwise noted, all reactions were magnetically stirred and performed under an inert atmosphere such as nitrogen or argon. 
     Unless otherwise noted, diethyl ether used in the experiments described below was Fisher ACS certified material and stabilized with BHT. 
     Unless otherwise noted, “degassed” refers to a solvent from which oxygen has been removed, generally by bubbling an inert gas such as nitrogen or argon through the solution for 10 to 15 minutes with an outlet needle to normalize pressure. 
     Unless otherwise noted, “concentrated” means evaporating the solvent from a solution or mixture using a rotary evaporator or vacuum pump. 
     Unless otherwise noted, “evaporated” means evaporating using a rotary evaporator or vacuum pump. 
     Unless otherwise noted, silica gel chromatography was carried out on an ISCO®, Analogix®, or Biotage® automated chromatography system using a commercially available cartridge as the column. Columns were usually filled with silica gel as the stationary phase. Reverse phase preparative HPLC conditions can be found at the end of the experimental section. Aqueous solutions were concentrated on a Genevac® evaporator or were lyophilized. 
     Unless otherwise noted, proton nuclear magnetic resonance ( 1 H NMR) spectra and proton-decoupled carbon nuclear magnetic resonance ( 13 C{ 1 H} NMR) spectra were recorded on 400, 500, or 600 MHz Bruker or Varian NMR spectrometers at ambient temperature. All chemical shifts (6) were reported in parts per million (ppm). Proton resonances were referenced to residual protium in the NMR solvent, which can include, but is not limited to, CDCl 3 , DMSO-d 6 , and MeOD-d 4 . Carbon resonances are referenced to the carbon resonances of the NMR solvent. Data are represented as follows: chemical shift, multiplicity (br=broad, br s=broad singlet, s=singlet, d=doublet, dd=doublet of doublets, ddd=doublet of doublet of doublets, t=triplet, q=quartet, m=multiplet), coupling constants (J) in Hertz (Hz), integration. 
     Intermediate 1: rac-3-(4-bromo-1H-pyrazol-1-yl)-2-methylbutan-2-ol 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 4-bromo-1H-pyrazole (1.00 g, 6.80 mmol) in DMF (3.40 ml) was added cesium carbonate (2.22 g, 6.80 mmol) and 2,2,3-trimethyloxirane (820 mg, 9.52 mmol). The mixture was stirred and heated at 90° C. for 4 h. The mixture was cooled to room temperature, filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 5-100% EtOAc in hexanes as eluent to afford rac-3-(4-bromo-1H-pyrazol-1-yl)-2-methylbutan-2-ol. LCMS (C 8 H 13 BrN 2 O) (ES, m/z) [M+H] + : 233, 235. 
     The intermediates in the following Table 1 were prepared in a manner similar to that of Intermediate 1 from the appropriate pyrazole and epoxide. 
     
       
         
           
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                   
                 Structure 
                 Observed m/z 
               
               
                 Intermediate 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 2 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 220, 222 
               
               
                   
               
               
                 3 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 234, 236 
               
               
                   
               
               
                 4 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 219, 221 
               
               
                   
               
               
                 5 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 231, 233 
               
               
                   
               
               
                 6 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 245, 247 
               
               
                   
               
               
                 7 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 ND 
               
               
                   
               
               
                 8 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 247, 249 
               
               
                   
               
               
                 9 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 247, 249 
               
               
                   
               
               
                 10 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 234, 236 
               
               
                   
               
            
           
         
       
     
     Intermediate 11: 1-((4-bromo-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 
     Step 1: (1-hydroxycyclobutyl)methyl methanesulfonate 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 1-(hydroxymethyl)cyclobutan-1-ol (9.00 g, 88.0 mmol) in DCM (260 mL) at 0° C. was added triethylamine (17.2 ml, 123 mmol) followed by methanesulfonyl chloride (7.0 mL, 90 mmol). The mixture was stirred at 0° C. for 10 min. The mixture was then warmed to room temperature and stirred for 15 min. The mixture was then partitioned with water. The layers were separated and the organic layer was washed with brine. The organic layer was dried over anhydrous MgSO 4 , filtered, and evaporated to afford (1-hydroxycyclobutyl)methyl methanesulfonate. 
     Step 2: 1-((4-bromo-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 
     
       
         
         
             
             
         
       
     
     To a solution of 4-bromo-1H-pyrazole (7.70 g, 52.4 mmol) in DMF (60 ml) at 0° C. was added NaH (60% in mineral oil, 2.30 g, 57.6 mmol) portionwise. The mixture was stirred at 0° C. under nitrogen for 30 min. To the mixture was added a solution of (1-hydroxycyclobutyl)methyl methanesulfonate (13.1 g, 72.8 mmol) in DMF (20 ml). The mixture was stirred and heated at 90° C. for 16 h. The mixture was quenched with water (70 mL), then extracted with EtOAc three times. The organic layer was dried over anhydrous MgSO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 0-30% EtOAc in petroleum ether as eluent, to afford 1-((4-bromo-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol. LCMS (C 8 H 11 BrN 2 O) (ES, m/z): 231, 233 [M+H] + . 
     Intermediate 12: 4-bromo-1-((3-methyloxetan-3-yl)methyl)-1H-pyrazole 
     Step 1: (3-methyloxetan-3-yl)methyl methanesulfonate 
     
       
         
         
             
             
         
       
     
     Step 1 of the synthesis of Intermediate 12 was conducted similar to step 1 of the synthesis of Intermediate 11 from the appropriate starting materials to afford (3-methyloxetan-3-yl)methyl methanesulfonate. 
     Step 2: 4-bromo-1-((3-methyloxetan-3-yl)methyl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a solution of 4-bromo-1H-pyrazole (5.04 g, 34.3 mmol) in DMF (114 mL) was added (3-methyloxetan-3-yl)methyl methanesulfonate (6.18 g, 34.3 mmol) and cesium carbonate (15.6 g, 48.0 mmol). The mixture was stirred and heated at 60° C. for 6 h. The solvents were evaporated. To the residue was added DCM (100 mL), and the mixture was filtered. The solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 0-100% EtOAc in hexane to afford 4-bromo-1-((3-methyloxetan-3-yl)methyl)-1H-pyrazole. LCMS (C 8 H 11 BrN 2 O) (ES, m/z): 231, 233 [M+H] + . 
     Intermediate 13, shown in the following Table 2, was prepared in a manner similar to that of Intermediate 12 from the appropriate starting materials. 
     
       
         
           
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
               
                   
                 Structure 
                 Observed m/z 
               
               
                 Intermediate 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 13 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 249, 251 
               
               
                   
               
            
           
         
       
     
     Intermediate 14: (1s,3s)-3-(4-bromo-1H-pyrazol-1-yl)-1-methylcyclobutanol 
     Step 1: 4-bromo-1-(5,8-dioxaspiro[3.4]octan-2-yl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a solution of 2-bromo-5,8-dioxaspiro[3.4]octane (0.500 g, 2.59 mmol) and 4-bromo-1H-pyrazole (0.761 g, 5.18 mmol) in DMF (2.6 mL) in an 8 mL vial was added potassium carbonate (1.07 g, 7.77 mmol) and 18-crown-6 (0.137 g, 0.518 mmol). The mixture was stirred and heated at 90° C. After 5 min, the mixture was cooled to room temperature, and to the mixture was added additional 4-bromo-1H-pyrazole (400 mg, 2.72 mmol). The mixture was stirred and heated at 90° C. for 48 h. The mixture was then cooled to room temperature and partitioned between EtOAc (25 mL) and water (25 mL). The layers were separated and the organic layer was washed with brine. The two aqueous layers were combined and extracted with EtOAc (15 mL). The organic layers were combined, washed with brine twice, dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 0-50% EtOAc in hexanes to afford 4-bromo-1-(5,8-dioxaspiro[3.4]octan-2-yl)-1H-pyrazole. 
     LCMS (C 9 H 12 BrN 2 O 2 ) (ES, m/z): 259, 261 [M+H] + . 
     Step 2: 3-(4-bromo-1H-pyrazol-1-yl)cyclobutanone 
     
       
         
         
             
             
         
       
     
     To a solution of 4-bromo-1-(5,8-dioxaspiro[3.4]octan-2-yl)-1H-pyrazole (270 mg, 1.042 mmol) and PPTS (131 mg, 0.521 mmol) in dioxane (2.6 mL) was added water (2.6 mL). The mixture was stirred and heated at 85° C. for 95 h. The mixture was cooled to room temperature. The mixture was partitioned between EtOAc and saturated aqueous sodium bicarbonate. The layers were separated, and the aqueous layer was extracted with EtOAc. The organic layers were combined, washed with brine, dried over anhydrous Na 2 SO 4 , filtered, and the solvents were evaporated. The residue was purified by silica gel chromatography with 0-100% EtOAc in hexane to afford 3-(4-bromo-1H-pyrazol-1-yl)cyclobutanone. LCMS (C 7 H 8 BrN 2 O) (ES, m/z): 215, 217 [M+H] + . 
     Step 3: (1s,3s)-3-(4-bromo-1H-pyrazol-1-yl)-1-methylcyclobutanol 
     
       
         
         
             
             
         
       
     
     A solution of 3-(4-bromo-1H-pyrazol-1-yl)cyclobutanone (129 mg, 0.600 mmol) in diethyl ether (3.5 ml) was cooled to 0° C. To the stirred mixture was added methylmagnesium bromide (3 M in diethyl ether, 0.240 ml, 0.720 mmol) dropwise. The mixture was stirred for 16 h, allowing the ice bath to expire. The mixture was partitioned between EtOAc and 20% aqueous citric acid and stirred for 2 h. The layers were separated and the aqueous layer was extracted with EtOAc. The organic layers were combined, washed with brine, dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 0-60% EtOAc in hexanes as eluent to afford (1s, 3s)-3-(4-bromo-1H-pyrazol-1-yl)-1-methylcyclobutanol. LCMS (C 8 H 12 BrN 2 O) (ES, m/z): 231, 233 [M+H] + . 
     Intermediate 15: 4-bromo-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a reaction vial were added 4-bromo-1H-pyrazole (1.50 g, 10.2 mmol), 4-iodotetrahydro-2H-pyran (2.16 g, 10.2 mmol), potassium carbonate (1.41 g, 10.2 mmol) and DMF (15 mL). The mixture was stirred and heated at 100° C. for 16 h. The mixture was purified by silica gel chromatography with 0-50% EtOAc in petroleum ether as eluent, to afford 4-bromo-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole. LCMS (C 8 H 11 BrN 2 O) (ES, m/z): 231, 233 [M+H] + . 
     Intermediate 16: mixture of 4-bromo-5-methyl-1-trityl-1H-pyrazole and 4-bromo-3-methyl-1-trityl-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a 200 mL round bottom flask was added 4-bromo-3-methyl-1H-pyrazole (1.00 g, 6.21 mmol) and THF (62.1 ml). The mixture was stirred under an atmosphere of nitrogen. The mixture was cooled at 0° C. To the mixture was added NaH (0.311 g, 7.76 mmol) portionwise. The mixture was slowly warmed to room temperature over 30 min. The mixture was then cooled at 0° C., and to the mixture was added trityl chloride (1.90 g, 6.83 mmol). The mixture was stirred for 16 h at room temperature. The mixture was quenched with water (60 mL) and diluted with EtOAc (60 mL). The layers were separated and the aqueous layer was further extracted with EtOAc (2×50 mL). The combined organic layers were dried over anhydrous MgSO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 0-25% EtOAc in hexanes as eluent to afford 4-bromo-3-methyl-1-trityl-1H-pyrazole and 4-bromo-5-methyl-1-trityl-1H-pyrazole as a mixture of regioisomers. LCMS (C 23 H 19 BrN 2 ) (ES, m/z): 425, 427 [M+Na] + . 
     Intermediate 17: 4-bromo-3-(difluoromethyl)-1-trityl-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 4-bromo-5-(difluoromethyl)-1H-pyrazole (250 mg, 1.27 mmol), trityl chloride (460 mg, 1.65 mmol) and pyridine (201 mg, 2.54 mmol) in DCM (4 mL) was added DMAP (15.5 mg, 0.127 mmol). The mixture was stirred at room temperature for 16 h. The mixture was washed with water (5 mL) and aqueous saturated NH 4 Cl (5 mL). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 0-50% EtOAc in petroleum ether as eluent to afford 4-bromo-5-(difluoromethyl)-1-trityl-1H-pyrazole. LCMS (C 23 H 17 BrF 2 N 2 ) (ES, m/z): 461, 463 [M+Na] + . 
     Intermediate 18: mixture of 4-bromo-3-(difluoromethyl)-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole and 4-bromo-5-(difluoromethyl)-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a solution of 4-bromo-5-(difluoromethyl)-1H-pyrazole (240 mg, 1.218 mmol) in DMF (60 ml) were added cesium carbonate (595 mg, 1.828 mmol) and tetrahydro-2H-pyran-4-yl methanesulfonate (329 mg, 1.828 mmol). The mixture was stirred and heated at 80° C. under nitrogen for 3 h. The mixture was cooled to room temperature and diluted with water (100 mL). The mixture was extracted with EtOAc three times. The organic layer was washed with water followed by brine, dried over anhydrous MgSO 4 , and filtered. The solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-90% EtOAc in hexanes as eluent to afford a mixture of 4-bromo-3-(difluoromethyl)-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole and 4-bromo-5-(difluoromethyl)-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole. 
     Intermediate 19: 2-(4-Bromo-1H-pyrazol-1-yl)-2-methylpropan-1-ol 
     Step 1: Methyl 2-(4-bromo-1H-pyrazol-1-yl)-2-methylpropanoate 
     
       
         
         
             
             
         
       
     
     To a solution of 4-bromo-1H-pyrazole (2.00 g, 13.6 mmol) in DMF (20 mL) was added methyl 2-bromo-2-methylpropanoate (1.76 mL, 13.6 mmol) followed by cesium carbonate (8.87 g, 27.2 mmol). The mixture was heated at 80° C. for 18 h. The mixture was filtered, and the filter cake was washed with DCM. The combined filtrates were evaporated. The resulting residue was purified by silica gel chromatography with 10% EtOAc in hexanes as eluent to afford methyl 2-(4-bromo-1H-pyrazol-1-yl)-2-methylpropanoate. LCMS (C 8 H 11 BrN 2 O 2 ) (ES, m/z): 247, 249 [M+H] + . 
     Step 2: 2-(4-bromo-1H-pyrazol-1-yl)-2-methylpropan-1-ol 
     
       
         
         
             
             
         
       
     
     To a solution of methyl 2-(4-bromo-1H-pyrazol-1-yl)-2-methylpropanoate (1.74 g, 7.04 mmol) in EtOH (35 ml) was added sodium borohydride (0.799 g, 21.1 mmol) at 0° C. The mixture was stirred at room temperature for 2 h. The mixture was diluted in DCM (50 mL), washed with water and brine solution. The organic layer was dried over anhydrous sodium sulfate, filtered, and the filtrate was evaporated to afford 2-(4-bromo-1H-pyrazol-1-yl)-2-methylpropan-1-ol. 
     LCMS (C 7 H 11 BrN 2 O) (ES, m/z): 219, 221 [M+H] + . 
     Intermediate 20 in the following Table 3 was prepared in a manner similar to that described for the synthesis of Intermediate 19 from the appropriate starting materials. 
     
       
         
           
               
               
               
             
               
                 TABLE 3 
               
               
                   
               
               
                   
                 Structure 
                 Observed m/z 
               
               
                 Intermediate 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 20 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 233, 235 
               
               
                   
               
            
           
         
       
     
     Intermediate 21: 1-(4-bromo-3-cyclopropyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     Step 1: 1-(3-cyclopropyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     Step 1 of the synthesis of Intermediate 21 was conducted in manner similar to that used in the synthesis of Intermediate 1 from the appropriate starting materials to afford 1-(3-cyclopropyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol. 
     Step 2: 1-(4-bromo-3-cyclopropyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a solution of 1-(3-cyclopropyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (1.55 g, 8.60 mmol) in DCM (86 mL) was added 1,3-dibromo-5,5-dimethylhydantoin (1.23 g, 4.30 mmol). The mixture was stirred at room temperature for 30 min. The solvents were evaporated, and the resulting residue was purified by silica gel chromatography with 10-90% EtOAc in hexanes to afford 1-(4-bromo-3-cyclopropyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol. LCMS (C 10 H 15 BrN 2 O) (ES, m/z): 259, 261 [M+H] + . 
     Intermediate 22: 4-bromo-1-ethyl-3-(((tetrahydro-2H-pyran-2-yl)oxy)methyl)-1H-pyrazole 
     Step 1: (4-bromo-1-ethyl-1H-pyrazol-3-yl)methanol 
     
       
         
         
             
             
         
       
     
     Step 1 of the synthesis of Intermediate 22 was conducted in a manner analogous to step 2 of the synthesis of Intermediate 21 from the appropriate starting materials to afford (4-bromo-1-ethyl-1H-pyrazol-3-yl)methanol. LCMS (C 6 H 9 BrN 2 O) (ES, m/z): 205, 207 [M+H] + . 
     Step 2: rac-4-bromo-1-ethyl-3-(((tetrahydro-2H-pyran-2-yl)oxy)methyl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a stirred solution of (4-bromo-1-ethyl-1H-pyrazol-3-yl)methanol (570 mg, 2.78 mmol) in DCM (27 mL) was added 3,4-dihydro-2H-pyran (468 mg, 5.56 mmol), followed by the addition of 4-methylbenzenesulfonic acid (polymer supported) (239 mg, 1.39 mmol). The mixture was stirred at room temperature for 2 h. The mixture was filtered, and the filtrate was loaded directly onto a silica gel column and purified with 0-60% EtOAc in hexane as eluent to afford rac-4-bromo-1-ethyl-3-(((tetrahydro-2H-pyran-2-yl)oxy)methyl)-1H-pyrazole. LCMS (C 11 H 17 BrN 2 O 2 ) (ES, m/z): 289, 291 [M+H] + . 
     Intermediate 23: rac-4-bromo-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole 
     Step 1: rac-(1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl methanesulfonate 
     
       
         
         
             
             
         
       
     
     To a stirred solution of rac-(1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methanol (8.00 g, 43.0 mmol) in DCM (150 mL) at 0° C. was added triethylamine (8.38 mL, 60.1 mmol) followed by methanesulfonyl chloride (4.02 mL, 51.5 mmol). The mixture was stirred at 0° C. for 10 min and then warmed to room temperature and stirred for 40 min. The mixture was then partitioned with water. The layers were separated, and the organic layer was washed with brine. The organic layer was dried over anhydrous MgSO 4 , filtered, and evaporated to afford rac-(1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl methanesulfonate, LCMS (C 11 H 20 O 5 S) (ES, m/z): 287 [M+Na] + . 
     Step 2: rac-4-bromo-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a solution of 4-bromo-1H-pyrazole (5.40 g, 36.7 mmol) in DMF (60 mL) at 0° C. was added NaH (60% in mineral oil, 1.62 g, 40.4 mmol) portionwise. The mixture was stirred at 0° C. under nitrogen for 1 h. To the mixture was added rac-(1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl methanesulfonate (10.7 g, 40.4 mmol). The mixture was stirred and heated at 90° C. under nitrogen for 16 h. The mixture was quenched with water (200 mL) and extracted with EtOAc three times. The combined organic layers were washed with water followed by brine. The organic layer was dried over anhydrous MgSO 4 , filtered, and evaporated. The resulting residue was purified by silica gel chromatography with 0-30% EtOAc in petroleum ether as eluent to afford rac-4-bromo-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole (Intermediate 23). LCMS (C 13 H 19 BrN 2 O 2 ) (ES, m/z): 315, 317 [M+H] + . 
     Intermediate 24 and Intermediate 25: 1-(4-bromo-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-bromo-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     The mixture of 1-(4-bromo-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-bromo-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 7) was separated by SFC (Chiral Technologies IG 21×250 mm column with 15% (MeOH w/0.1% NH 4 OH modifier) as cosolvent) to afford 1-(4-bromo-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 24, first eluting peak) and 1-(4-bromo-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 25, second eluting peak). 
     For Intermediate 24: LCMS (C 13 H 19 BrN 2 O 2 ) (ES, m/z): 233, 235 [M+H] + . 
     For Intermediate 25: LCMS (C 8 H 13 BrN 2 O) (ES, m/z): 233, 235 [M+H] + . 
     Intermediate 26 and Intermediate 27 in the following Table 4 were prepared in a manner analogous to the preparation of Intermediate 24 and Intermediate 25 by SFC separation of the racemic mixture Intermediate 1. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 4 
               
               
                   
               
               
                   
                   
                   
                 Observed 
               
               
                 Inter- 
                 Structure 
                 SFC 
                 m/z 
               
               
                 mediate 
                 Name 
                 Conditions 
                 [M + H] +   
               
               
                   
               
             
            
               
                 26 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AD-H 50 × 250 mm column with 35% MeOH as co-solvent 
                 233, 235 
               
               
                   
               
               
                   
                 (S or R)-3-(4-bromo-1H-pyrazol- 
                   
                   
               
               
                   
                 1-yl)-2-methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 27 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AD-H 50 × 250 mm column with 35% MeOH as co-solvent 
                 233, 235 
               
               
                   
               
               
                   
                 (R or S)-3-(4-bromo-1H-pyrazol- 
                   
                   
               
               
                   
                 1-yl)-2-methylbutan-2-ol 
               
               
                   
               
            
           
         
       
     
     Intermediate 28: rac-2-(4-bromo-1H-pyrazol-1-yl)cyclobutan-1-one 
     
       
         
         
             
             
         
       
     
     To a solution of 2-bromocyclobutanone (16.2 g, 109 mmol) in MeCN (30 mL) was added 4-bromo-1H-pyrazole (8.00 g, 54.4 mmol) and potassium carbonate (30.1 g, 218 mmol). The mixture was stirred at 20° C. for 10 h. The mixture was filtered, and the solvents of the filtrate were evaporated. The residue was purified by reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm MeCN/water (with 0.1% TFA modifier) as eluent) to afford rac-2-(4-bromo-1H-pyrazol-1-yl)cyclobutanone. LCMS (C 7 H 7 BrN 2 O) (ES, m/z) [M+H] + : 215, 217. 
     Intermediate 29: 2-(4-bromo-1H-pyrazol-1-yl)-1-methylcyclobutan-1-ol 
     
       
         
         
             
             
         
       
     
     Methylmagnesium bromide (0.248 ml, 0.744 mmol, 3 M in diethyl ether) was added to a stirred mixture of rac-2-(4-bromo-1H-pyrazol-1-yl)cyclobutanone (Intermediate 28) (80.0 mg, 0.372 mmol) in THF (2 mL) at −78° C., and the mixture was stirred at that temperature for 3 h. The reaction was quenched with aqueous saturated NH 4 Cl (2 mL), and the desired layer was extracted from the mixture with EtOAc (2×20 mL). The combined organic layers were dried over anhydrous Na 2 SO 4 , filtered, and evaporated. The resulting residue was purified by preparative silica gel TLC with 30% EtOAc in petroleum ether as eluent to afford 2-(4-bromo-1H-pyrazol-1-yl)-1-methylcyclobutanol. LCMS (C 8 H 11 BrN 2 O) (ES, m/z) [M+H] + : 231, 233. 
     Intermediate 30: rac-4-bromo-1-(2,2-dimethoxycyclobutyl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of trimethoxymethane (592 mg, 5.58 mmol) and rac-2-(4-bromo-1H-pyrazol-1-yl)cyclobutanone (Intermediate 28) (600 mg, 2.79 mmol) in MeOH (5 mL) was added 4-methylbenzenesulfonic acid hydrate (53.1 mg, 0.279 mmol). The mixture was stirred at 28° C. for 12 h. The mixture was diluted with EtOAc (50 mL) and washed with water (30 mL). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-10% EtOAc in petroleum ether as eluent to afford rac-4-bromo-1-(2,2-dimethoxycyclobutyl)-1H-pyrazole. 
     LCMS (C 9 H 13 BrN 2 O 2 ) (ES, m/z) [M+H] + : 261, 263. 
     Intermediate 31: mixture of rac-4-bromo-5-methyl-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole and rac-4-bromo-3-methyl-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a stirred solution of rac-(1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methanol (1.00 g, 5.37 mmol), 4-bromo-5-methyl-1H-pyrazole (0.864 g, 5.37 mmol) and triphenylphosphine (1.41 g, 5.37 mmol) in THF (10.2 mL) was added diisopropyl diazene-1,2-dicarboxylate (1.09 g, 5.37 mmol). The mixture was stirred and heated at 60° C. for 16 h. The solvents were evaporated. The resulting residue was purified by silica gel chromatography with 0-80% EtOAc in hexane to afford a mixture of rac-4-bromo-5-methyl-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole and rac-4-bromo-3-methyl-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole. LCMS (C 14 H 21 BrN 2 O 2 ) (ES, m/z): 329, 331 [M+H] + . 
     Intermediate 32: rac-4-bromo-1-(2-((tetrahydro-2H-pyran-2-yl)oxy)cyclopentyl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2-(4-bromo-1H-pyrazol-1-yl)cyclopentanol (Intermediate 5) (3.00 g, 13.0 mmol) in DCM (45 mL) was added 3,4-dihydro-2H-pyran (2.4 mL, 26 mmol), followed by 4-methylbenzenesulfonic acid (polymer-bound, 2.0 g). The mixture was stirred at room temperature for 16 h. The mixture was filtered, and the solvents of the filtrate were evaporated. The residue was purified by reversed-phase C18 chromatography with 0-100% MeCN in water as eluent to afford rac-4-bromo-1-(2-((tetrahydro-2H-pyran-2-yl)oxy)cyclopentyl)-1H-pyrazole. LCMS (C 13 H 19 BrN 2 O 2 ) (ES, m/z): 315, 317 [M+H] + . 
     Intermediate 33: 1-(4-amino-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     Step 1: 2-methyl-1-(4-nitro-1H-pyrazol-1-yl)propan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 500 mL round bottom flask was added 4-nitro-1H-pyrazole (15.0 g, 133 mmol), cesium carbonate (64.8 g, 199 mmol), and DMF (195 mL). To the mixture was added 2,2-dimethyloxirane (23.6 mL, 265 mmol). The mixture was heated at 80° C. for 16 h. The mixture was cooled to room temperature. The mixture was filtered and washed with EtOAc. The solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-80% EtOAc in hexanes, yielding 2-methyl-1-(4-nitro-1H-pyrazol-1-yl)propan-2-ol. LCMS (C 7 H 11 N 3 O 3 ) (ES, m/z): 186 [M+H] + . 
     Step 2: 1-(4-amino-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 500 mL flask was added 2-methyl-1-(4-nitro-1H-pyrazol-1-yl)propan-2-ol (18.8 g, 102 mmol), 10% palladium on carbon (1.08 g, 1.01 mmol), and EtOAc (300 mL). The mixture was degassed under vacuum and refilled with nitrogen three times. The mixture was degassed and refilled with hydrogen from a balloon. The mixture was stirred under an atmosphere of hydrogen for 21 h. The mixture was filtered through Celite® (diatomaceous earth). The solvents of the filtrate were evaporated, yielding 1-(4-amino-1H-pyrazol-1-yl)-2-methylpropan-2-ol. LCMS (C 7 H 13 N 3 O) (ES, m/z): 156 [M+H] + . 
     Intermediate 34: 2-(4-amino-1H-pyrazol-1-yl)-2-methylpropan-1-ol 
     Step 1: ethyl 2-methyl-2-(4-nitro-1H-pyrazol-1-yl)propanoate 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 4-nitro-1H-pyrazole (3.00 g, 26.5 mmol) and ethyl 2-bromo-2-methylpropanoate (5.69 g, 29.2 mmol) in DMF (50 mL) was added K 2 CO 3  (11.00 g, 80.00 mmol). The mixture was stirred and heated at 80° C. for 10 h. The mixture was cooled, filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography 5-20% EtOAc in petroleum ether as eluent to afford ethyl 2-methyl-2-(4-nitro-1H-pyrazol-1-yl)propanoate. LCMS (C 9 H 13 N 3 O 4 ) (ES, m/z): 228 [M+H] + . 
     Step 2: 2-methyl-2-(4-nitro-1H-pyrazol-1-yl)propan-1-ol 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of ethyl 2-methyl-2-(4-nitro-1H-pyrazol-1-yl)propanoate (3.00 g, 13.2 mmol) in EtOH (50 mL) was added NaBH 4  (0.999 g, 26.4 mmol). The mixture was stirred at room temperature for 2 h. The mixture was diluted with water (40 mL) and extracted with EtOAc (2×50 mL). The combined organic layers were dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated to afford 2-methyl-2-(4-nitro-1H-pyrazol-1-yl) propan-1-ol. 
     Step 3: 2-(4-amino-1H-pyrazol-1-yl)-2-methylpropan-1-ol 
     
       
         
         
             
             
         
       
     
     Step 3 of the synthesis of Intermediate 34 was conducted in a manner similar to that of step 2 of the synthesis of Intermediate 33, using 2-methyl-2-(4-nitro-1H-pyrazol-1-yl) propan-1-ol as the starting material, to afford 2-(4-amino-1H-pyrazol-1-yl)-2-methylpropan-1-ol. LCMS (C 7 H 13 N 3 O) (ES, m/z): 156 [M+H] + . 
     Intermediate 35: 2-amino-5-fluoro-4-methoxybenzonitrile 
     Step 1: 2-bromo-4-fluoro-5-methoxyaniline 
     
       
         
         
             
             
         
       
     
     A solution of 4-fluoro-3-methoxyaniline (350.0 g, 2.48 mol) in EtOAc (3.5 L) was cooled at 0-5° C. To the mixture was added tetra-n-butylammonium tribromide (14.0 kg, 2.90 mol) portionwise. The mixture was warmed to 15° C., and stirred at that temperature for 1 h. The mixture was adjusted to pH 8 with saturated aqueous Na 2 CO 3 . The mixture was extracted with EtOAc and the combined organic layers were washed with water (2×1.5 L) and dried with anhydrous Na 2 SO 4 . The solids were removed by filtration, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-100% EtOAc in petroleum ether as eluent to afford 2-bromo-4-fluoro-5-methoxyaniline. LCMS (C 7 H 7 BrFNO) (ES, m/z): 220, 222 [M+H] + . 
     Step 2: 2-amino-5-fluoro-4-methoxybenzonitrile 
     
       
         
         
             
             
         
       
     
     To a solution of 2-bromo-4-fluoro-5-methoxyaniline (300 g, 1.36 mol) in DMF (2.1 L) was added Zn(CN) 2  (327 g, 2.78 mol) and Pd(PPh 3 ) 4  (90.0 g, 0.0778 mol). The mixture was degassed under vacuum and purged with nitrogen. The mixture was stirred and heated at 130° C. for 1 h under nitrogen. The mixture was poured into ice water (4 L). The mixture was extracted with EtOAc (3 L, 2 L, 1 L), and the combined organic layers were washed with brine (2 L, 1.5 L). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-100% EtOAc in petroleum ether as eluent to afford 2-amino-5-fluoro-4-methoxybenzonitrile. LCMS (C 8 H 7 FN 2 O) (ES, m/z): 167 [M+H] + . 
     Intermediate 36: 2-amino-4-chloro-5-fluorobenzonitrile 
     
       
         
         
             
             
         
       
     
     To a 20 mL microwave vial was added 2-bromo-5-chloro-4-fluoroaniline (1.00 g, 4.46 mmol), copper(I) cyanide (0.472 g, 4.90 mmol), and NMP (8 mL). The mixture was stirred and heated at 180° C. in a microwave for 1 h. The mixture was diluted in diethyl ether (100 mL) and filtered through Celite® (diatomaceous earth). The filtrate was washed with water (3×100 mL). The organic layer was dried over anhydrous magnesium sulfate, filtered, and the solvents of the filtrate were evaporated, to afford 2-amino-4-chloro-5-fluorobenzonitrile (LCMS (C 7 H 4 ClFN 2 ) (ES, m/z): 171 [M+H] + . 
     Intermediate 37: 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5-fluoro-4-methoxybenzonitrile 
     Step 1: 1-(2-Cyano-4-fluoro-5-methoxyphenyl)-3-(2,4-dimethoxybenzyl)urea 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added 2-amino-5-fluoro-4-methoxybenzonitrile (Intermediate 35) (817 mg, 4.92 mmol), DCM (6 mL), and pyridine (1 mL). The mixture was stirred. To the mixture was added 1-(isocyanatomethyl)-2,4-dimethoxybenzene (1425 mg, 7.380 mmol). The mixture was stirred and heated at 40° C. for 16 h. The solids were collected by filtration and washed with MeOH (3×3 mL), to afford 1-(2-cyano-4-fluoro-5-methoxyphenyl)-3-(2,4-dimethoxybenzyl)urea. 
     Step 2: 2-((((2,4-Dimethoxybenzyl)imino)methylene)amino)-5-fluoro-4-methoxybenzonitrile 
     
       
         
         
             
             
         
       
     
     To a 100 mL round bottom flask was added 1-(2-cyano-4-fluoro-5-methoxyphenyl)-3-(2,4-dimethoxybenzyl)urea (1.16 g, 3.22 mmol), triphenylphosphine (1.69 g, 6.44 mmol), triethylamine (1.80 ml, 12.9 mmol), and DCM (25 mL). The mixture was stirred and cooled at 0° C. To the mixture was added a solution of carbon tetrabromide (2.14 g, 6.44 mmol) in DCM (5 mL) dropwise. After 30 min, the mixture was concentrated. The resulting residue was purified by silica gel chromatography with 0-70% EtOAc in hexanes as eluent, to afford 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5-fluoro-4-methoxybenzonitrile. LCMS (C 18 H 16 FN 3 O 3 ) (ES, m/z) [M+Na] + : 364. 
     The intermediates in the following Table 5 were prepared in a manner similar to that of Intermediate 37 from the appropriate intermediates and starting materials. 
     
       
         
           
               
               
               
             
               
                 TABLE 5 
               
               
                   
               
               
                   
                   
                 Ob- 
               
               
                   
                   
                 served 
               
               
                   
                   
                 m/z 
               
               
                 Inter- 
                 Structure 
                 [M + 
               
               
                 mediate 
                 Name 
                 Na] +   
               
               
                   
               
             
            
               
                 38 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 364 
               
               
                   
               
               
                   
                 2-((((2,3-dimethoxybenzyl)imino)methylene)amino)-5- 
                   
               
               
                   
                 fluoro-3-methoxybenzonitrile 
                   
               
               
                   
               
               
                 39 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 368 
               
               
                   
               
               
                   
                 4-chloro-2-((((2,4- 
                   
               
               
                   
                 dimethoxybenzyl)imino)methylene)amino)-5- 
                   
               
               
                   
                 fluorobenzonitrile 
                   
               
               
                   
               
               
                 40 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 348 
               
               
                   
               
               
                   
                 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5- 
                   
               
               
                   
                 fluoro-4-methylbenzonitrile 
                   
               
               
                   
               
               
                 41 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 352 
               
               
                   
               
               
                   
                 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)- 
                   
               
               
                   
                 3,5-difluorobenzonitrile 
                   
               
               
                   
               
               
                 42 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 384 
               
               
                   
               
               
                   
                 3,5-dichloro-2-((((2,4- 
                   
               
               
                   
                 dimethoxybenzyl)imino)methylene)amino)benzonitrile 
               
               
                   
               
            
           
         
       
     
     Intermediate 43: 1-(tert-butyl) 3-ethyl (R)-piperidine-1,3-dicarboxylate 
     
       
         
         
             
             
         
       
     
     A solution of (R)-ethyl piperidine-3-carboxylate (200.0 g, 1270 mmol), triethylamine (257.5 g, 2540 mmol) and DMAP (15.5 g, 130 mmol) in DCM (2 L) was cooled at 0° C. To the mixture was added di-tert-butyl dicarbonate (305.4 g, 1400 mmol) portionwise. The mixture was stirred at room temperature for 3 h. Then the organic layer was washed with aqueous saturated sodium bicarbonate (3×1 L). The combined organic layers were dried over anhydrous MgSO 4 , filtered, and the solvents of the filtrate were evaporated to afford 1-(tert-butyl) 3-ethyl (R)-piperidine-1,3-dicarboxylate. 
     Intermediate 44 in the following Table 6 was prepared in a manner similar to that of Intermediate 43 from the appropriate starting materials. 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 6 
               
               
                   
                   
               
               
                   
                   
                 Structure 
                 Observed m/z 
               
               
                   
                 Intermediate 
                 Name 
                 [M + Na] +   
               
               
                   
                   
               
             
            
               
                   
                 44 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 280 
               
               
                   
                   
               
               
                   
                   
                 1-(tert-butyl) 3-methyl azepane- 
                   
               
               
                   
                   
                 1,3-dicarboxylate 
               
               
                   
                   
               
            
           
         
       
     
     Intermediate 45: 1-(tert-butyl) 3-methyl 5-methylpiperidine-1,3-dicarboxylate 
     Step 1: 5-methylpiperidine-3-carboxylic acid 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 5-methylnicotinic acid (10 g, 72.9 mmol) and concentrated aqueous HCl (0.599 mL, 7.29 mmol) in MeOH (100 mL) at 20° C. was added platinum (IV) oxide (1.67 g, 7.29 mmol). The mixture was degassed and purged with nitrogen then pressurized to 50 psi with hydrogen. The mixture was stirred for 10 h. The mixture was filtered, and the solvents of the filtrate were evaporated to afford the 5-methylpiperidine-3-carboxylic acid. 
     Step 2: 1-(tert-butoxycarbonyl)-5-methylpiperidine-3-carboxylic acid 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of di-tert-butyl dicarbonate (5.84 ml, 25.1 mmol) and 5-methylpiperidine-3-carboxylic acid (3.00 g, 21.0 mmol) in MeCN (20 mL) and water (20 mL) at 20° C. was added sodium bicarbonate (7.04 g, 84.0 mmol). The mixture was stirred at 20° C. for 5 h. The mixture was diluted with water (20 mL), adjusted with concentrated aqueous HCl to pH 5, and extracted with EtOAc (3×30 mL). The combined organic layers were dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated to afford 1-(tert-butoxycarbonyl)-5-methylpiperidine-3-carboxylic acid. LCMS (C 12 H 21 NO 4 ) (ES, m/z) [M+H] + : 244. 
     Step 3: 1-(tert-butyl) 3-methyl 5-methylpiperidine-1,3-dicarboxylate 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 1-(tert-butoxycarbonyl)-5-methylpiperidine-3-carboxylic acid (5.00 g, 20.5 mmol) in DCM (10 mL) and MeOH (10 mL) at 0° C. was added trimethylsilyl-diazomethane (15.4 mL, 30.8 mmol). The mixture was stirred at room temperature for 2 h. The solvents were evaporated to afford 1-tert-butyl 3-methyl 5-methylpiperidine-1,3-dicarboxylate. LCMS (C 13 H 23 NO 4 ) (ES, m/z) [M+H] + : 258. 
     Intermediate 46: 1-(tert-butyl) 3-methyl 4-methylpiperidine-1,3-dicarboxylate 
     Step 1: Methyl 4-methylpiperidine-3-carboxylate 
     
       
         
         
             
             
         
       
     
     Step 1 of the synthesis of Intermediate 46 was conducted in a manner similar to that of step 1 of the synthesis of Intermediate 45 from the appropriate starting materials to afford methyl 4-methylpiperidine-3-carboxylate. LCMS (C 8 H 15 NO 2 ) (ES, m/z) [M+H] + : 158. 
     Step 2: 1-(tert-butyl) 3-methyl 4-methylpiperidine-1,4-dicarboxylate 
     
       
         
         
             
             
         
       
     
     Step 2 of the synthesis of Intermediate 46 was conducted in a manner similar to step 2 of the synthesis of Intermediate 45 from the appropriate starting materials, with the exception that the crude material was purified by silica gel chromatography with 0-30% EtOAc in petroleum ether as eluent to afford to 1-(tert-butyl) 3-methyl 4-methylpiperidine-1,4-dicarboxylate. Intermediate 47: mixture of rac,cis-1-(tert-butyl) 3-ethyl-5-fluoropiperidine-1,3-dicarboxylate and rac,trans-1-(tert-butyl) 3-ethyl-5-fluoropiperidine-1,3-dicarboxylate 
     
       
         
         
             
             
         
       
     
     To a 250 mL round bottom flask containing rac, cis-1-(tert-butyl) 3-ethyl-5-fluoropiperidine-1,3-dicarboxylate (2.00 g, 7.26 mmol) was added EtOH (73 mL). To the mixture was added sodium tert-butoxide (7.26 mL, 14.5 mmol) (2 M solution in THF) dropwise with stirring. The mixture was stirred at room temperature for 3 h. The mixture was concentrated to about 10 mL of volume. To the mixture was added EtOAc (10 mL). The solvents were evaporated. The residue was dissolved in EtOAc (60 mL) and washed with water (3×20 mL). The organic layer was dried over anhydrous sodium sulfate, filtered, and the solvents were evaporated to afford a mixture of rac,cis-1-(tert-butyl) 3-ethyl-5-fluoropiperidine-1,3-dicarboxylate and rac,trans-1-(tert-butyl) 3-ethyl-5-fluoropiperidine-1,3-dicarboxylate. 
     Intermediate 48 and 49: methyl (3S,6R)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carboxylate and methyl (3R,6S)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carboxylate 
     
       
         
         
             
             
         
       
     
     A 100 mL flask was charged with 1-(4-amino-1H-pyrazol-1-yl)-2-methylpropan-2-ol (4.66 g, 30.0 mmol), methyl 2-methylene-5-oxohexanoate 1  (3.12 g, 20.0 mmol), and LiBF 4  (1.88 g, 20.0 mmol). To the flask was added TFE (31.2 mL). The flask was fitted with a reflux condenser, which had an inlet for nitrogen. The mixture was heated at reflux for 48 h. The mixture was cooled to room temperature, and to the mixture was added 10% palladium on carbon (0.639 g, 6.00 mmol). The mixture was placed under an atmosphere of hydrogen and stirred at room temperature for 6 h. The mixture was filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-4% MeOH in DCM as eluent, yielding the racemate with cis relative stereochemistry. The racemic mixture was resolved by chiral SFC (Chiral Technologies AD-H 21×250 mm column with 15% (MeOH w/0.1% NH 4 OH modifier) as cosolvent), to afford methyl (3S,6R)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carboxylate (Intermediate 48, first eluting peak) and methyl (3R,6S)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carboxylate (Intermediate 49, second eluting peak). 
     For Intermediate 48: LCMS (C 15 H 25 N 3 O 3 ) (ES, m/z): 296 [M+H] + . For Intermediate 49: LCMS (C 15 H 25 N 3 O 3 ) (ES, m/z): 296 [M+H] + .  1 Bizet, V.; Lefebvre, V.; Baudoux, J.; Lasne, M.; Boulange, A.; Leleu, S.; Franck, X.; Rouden, J. Eur. J. Org. Chem. 2011, 4170. 
     The intermediates in the following Table 7 were prepared in a manner similar to that of Intermediate 48 and Intermediate 49 from the appropriate intermediates and starting materials, with the exception that these compounds were isolated as racemic mixtures of diastereomers that were not resolved by SFC separation. 
     
       
         
           
               
               
               
             
               
                 TABLE 7 
               
               
                   
               
               
                   
                 Structure 
                 Observed 
               
               
                 Intermediate 
                 Name 
                 m/z [M + H] +   
               
               
                   
               
             
            
               
                 50 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 310 
               
               
                   
               
               
                   
                 ethyl 1-(1-(1-hydroxy-2- 
                   
               
               
                   
                 methylpropan-2-yl)-1H-pyrazol-4- 
                   
               
               
                   
                 yl)-6-methylpiperidine-3-carboxylate 
                   
               
               
                   
               
               
                 51 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 310 
               
               
                   
               
               
                   
                 methyl 1-(1-(2-hydroxy-2- 
                   
               
               
                   
                 methylpropyl)-3-methyl-1H-pyrazol-4- 
                   
               
               
                   
                 yl)-6-methylpiperidine-3-carboxylate 
               
               
                   
               
            
           
         
       
     
     Intermediate 52: ethyl 6-ethyl-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)-2-oxopiperidine-3-carboxylate 
     Step 1: diethyl 2-(3-oxopentyl)malonate 
     
       
         
         
             
             
         
       
     
     A mixture of diethyl malonate (10.0 g, 62.4 mmol), pent-1-en-3-one (5.78 g, 68.7 mmol) and potassium carbonate (0.863 g, 6.24 mmol) was stirred at room temperature in a sealed tube for 3 days. The resulting mixture was filtered to provide the filtrate, which is neat diethyl 2-(3-oxopentyl) malonate. LCMS (C 12 H 20 O 5 ) (ES, m/z): 245 [M+H] + . The crude material was used without further purification. 
     Step 2: rac-diethyl 2-(3-((1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)amino)pentyl)malonate 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 1-(4-amino-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 33) (2.00 g, 12.9 mmol) in DCM (129 mL) was added diethyl 2-(3-oxopentyl)malonate (6.93 g, 28.4 mmol) and AcOH (0.077 mL, 1.3 mmol). The mixture was stirred at room temperature for 30 min. To the mixture was added sodium cyanoborohydride (1.62 g, 25.8 mmol) portionwise. The mixture was stirred at room temperature for an additional 30 min. The mixture was quenched with 1 M aqueous HCl (150 mL). The organic layer was separated, and the aqueous layer was extracted with DCM twice more. The combined organic layers were dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated to afford rac-diethyl 2-(3-((1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)amino)pentyl)malonate. LCMS (C 19 H 33 N 3 O 5 ) (ES, m/z): 384 [M+H] + . 
     Step 3: ethyl 6-ethyl-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)-2-oxopiperidine-3-carboxylate 
     
       
         
         
             
             
         
       
     
     To a solution of rac-diethyl 2-(3-((1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)amino)pentyl)malonate (1.70 g, 4.43 mmol) in toluene (22 mL) was added AcOH (0.530 mL, 8.87 mmol). The mixture was stirred at 90° C. for 2 days. The mixture was cooled to room temperature, and the solvents were evaporated. The residue was purified by silica gel chromatography with 0-100% EtOAc in hexanes as eluent to afford ethyl 6-ethyl-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)-2-oxopiperidine-3-carboxylate. LCMS (C 17 H 27 N 3 O 4 ) (ES, m/z): 338 [M+H] + . 
     Intermediate 53: ethyl 3-hydroxycyclohexanecarboxylate 
     
       
         
         
             
             
         
       
     
     To a solution of ethyl 3-oxocyclohexanecarboxylate (2.00 g, 11.7 mmol) in THF (20 mL) was added a solution of sodium borohydride (0.889 g, 23.5 mmol) in THF (10 mL) at 0° C. The mixture was stirred at 0° C. for 2 h. To the mixture was added water (10 mL), and the mixture was extracted with EtOAc (3×15 mL). The combined organic layers were dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 10-50% EtOAc in petroleum ether as eluent to afford ethyl 3-hydroxycyclohexanecarboxylate. 
     Intermediate 54: tert-butyl (R)-3-(hydrazinecarbonyl)piperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     The solution of (R)-1-tert-butyl 3-ethyl piperidine-1,3-dicarboxylate (320.0 g, 1243 mmol) and hydrazine hydrate (311.3 g, 6217 mmol) in EtOH (1.6 L) was stirred and heated at 80° C. for 16 h. The solvents were evaporated. The resulting residue was purified by silica gel chromatography eluting with DCM to afford tert-butyl (R)-3-(hydrazinecarbonyl)piperidine-1-carboxylate. LCMS (C 11 H 21 N 3 O 3 ) (ES, m/z): 244 [M+H] + . 
     The intermediates in the following Table 8 were prepared in a manner similar to that of Intermediate 54 from the appropriate intermediates and starting materials. 
     
       
         
           
               
               
               
             
               
                 TABLE 8 
               
               
                   
               
               
                   
                 Structure 
                 Observed 
               
               
                 Intermediate 
                 name 
                 m/z [M + H] +   
               
               
                   
               
             
            
               
                 55 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 258 
               
               
                   
               
               
                   
                 tert-butyl 3-(hydrazinecarbonyl)azepane-1-carboxylate 
                   
               
               
                   
               
               
                 56 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 258 
               
               
                   
               
               
                   
                 tert-butyl 3-(hydrazinecarbonyl)-5-methylpiperidine-1-carboxylate 
                   
               
               
                   
               
               
                 57 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 258 
               
               
                   
               
               
                   
                 tert-butyl 3-(hydrazinecarbonyl)-4-methylpiperidine-1-carboxylate 
                   
               
               
                   
               
               
                 58 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 206 [M + H − C 4 H 8 ] +   
               
               
                   
               
               
                   
                 tert-butyl 3-fluoro-4-(hydrazinecarbonyl)piperidine-1-carboxylate 
                   
               
               
                   
               
               
                 59 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 206 [M + H − C 4 H 8 ] +   
               
               
                   
               
               
                   
                 mixture of tert-butyl (3R,5R and 3S,5S)-3-fluoro-5- 
                   
               
               
                   
                 (hydrazinecarbonyl)piperidine-1-carboxylate and tert- 
                   
               
               
                   
                 butyl (3S,5R and 3R,5S)-3-fluoro-5- 
                   
               
               
                   
                 (hydrazinecarbonyl)piperidine-1-carboxylate 
                   
               
               
                   
               
               
                 60 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 248 
               
               
                   
               
               
                   
                 tert-butyl 3-fluoro-3-(hydrazinecarbonyl)pyrrolidine-1-carboxylate 
                   
               
               
                   
               
               
                 61 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 296 
               
               
                   
               
               
                   
                 (3R,6S)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol- 
                   
               
               
                   
                 4-yl)-6-methylpiperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 62 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 296 
               
               
                   
               
               
                   
                 1-(1-(1-hydroxy-2-methylpropan-2-yl)-1H-pyrazol-4- 
                   
               
               
                   
                 yl)-6-methylpiperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 63 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 310 
               
               
                   
               
               
                   
                 1-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol- 
                   
               
               
                   
                 4-yl)-6-methylpiperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 64 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 324 
               
               
                   
               
               
                   
                 6-ethyl-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4- 
                   
               
               
                   
                 yl)-2-oxopiperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 65 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 159 
               
               
                   
               
               
                   
                 3-hydroxycyclohexane-1-carbohydrazide 
                   
               
               
                   
               
               
                 66 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 258 
               
               
                   
               
               
                   
                 tert-butyl 5-(hydrazinecarbonyl)-2-methylpiperidine-1-carboxylate 
               
               
                   
               
            
           
         
       
     
     Intermediate 67: (R)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide 
     Step 1: ethyl 1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carboxylate 
     
       
         
         
             
             
         
       
     
     A 40 mL reaction vial was charged with ethyl 1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carboxylate (1.00 g, 6.36 mmol) and THF (15 mL). To the mixture was added 4-bromo-1-methyl-1H-pyrazole (4.96 mL, 48.0 mmol), followed by tBuXPhos-Pd G3 (2.02 g, 2.54 mmol) and sodium tert-butoxide (4.61 g, 48.0 mmol). Nitrogen was bubbled through the mixture for 10 min. The vial was sealed and heated at 65° C. for 24 h. The mixture was cooled to room temperature and diluted with EtOAc (40 mL). The mixture was filtered through Celite® (diatomaceous earth). The solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-10% MeOH in DCM to afford ethyl 1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carboxylate. LCMS (C 12 H 19 N 3 O 2 ) (ES, m/z): 238 [M+H] + . 
     Step 2: (R)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide 
     
       
         
         
             
             
         
       
     
     A round bottom flask was charged with ethyl 1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carboxylate (7.72 g, 32.5 mmol) and EtOH (77 mL). To the mixture was added hydrazine hydrate (31.7 mL, 651 mmol). The round bottom flask was fitted with a reflux condenser, and the mixture was heated at 80° C. for 16 h. The mixture was cooled to room temperature, and the solvents were evaporated to afford (R and S)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide (Intermediate 67). The racemic mixture was resolved by chiral SFC separation (Chiral Technologies AD-H 21×250 mm column with 40% (MeOH w/0.25% DEA modifier) as co-solvent to afford (R or S)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide as the first eluting peak and (S or R)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide as the second eluting peak corresponding to Intermediate 67a and Intermediate 67b, respectively. LCMS (C 10 H 17 N 5 O) (ES, m/z): 224 [M+H] + . 
     Intermediate 68 in the following Table 9 was prepared in a manner similar to that of Intermediate 67, with the exception that no SFC separation was conducted. Thus, the compound was isolated as a mixture of isomers. 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 9 
               
               
                   
                   
               
               
                   
                   
                 Structure 
                 Observed 
               
               
                   
                 Intermediate 
                 Name 
                 m/z [M + H] +   
               
               
                   
                   
               
             
            
               
                   
                 68 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 296 
               
               
                   
                   
               
               
                   
                   
                 1-(1-(2-hydroxy-2-methylpropyl)- 
                   
               
               
                   
                   
                 1H-pyrazol-4-yl)-6- 
                   
               
               
                   
                   
                 methylpiperidine-3-carbohydrazide 
               
               
                   
                   
               
            
           
         
       
     
     Intermediate 69: tert-butyl (R)-3-(hydrazinecarbonyl)pyrrolidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a 100 mL round bottom flask was added (R)-1-(tert-butoxycarbonyl)pyrrolidine-3-carboxylic acid (2.00 g, 9.29 mmol) and THF (18.6 mL). To the mixture was added 1,1′-carbonyldiimidazole (1.96 g, 12.1 mmol). The mixture was heated at 60° C. for 30 min. The mixture was cooled to room temperature and transferred to a stirring mixture of hydrazine hydrate (0.447 g, 13.9 mmol) in THF (10 mL) dropwise over 25 min. The mixture was stirred at room temperature for 2 h. The mixture was quenched with water (50 mL) and extracted with EtOAc (2×60 mL). The combined organic layers were dried over anhydrous MgSO 4 , filtered, and the solvents of the filtrate were evaporated to afford (R)-tert-butyl 3-(hydrazinecarbonyl)pyrrolidine-1-carboxylate. LCMS (C 10 H 19 N 3 O 3 ) (ES, m/z): 230 [M+H] + . The intermediates in the following Table 9A were prepared in a manner similar to that of the preparation of Intermediate 60. 
     
       
         
           
               
               
               
             
               
                 TABLE 9A 
               
               
                   
               
               
                   
                 Structure 
                 Observed 
               
               
                 Intermediate 
                 Name 
                 m/z [M + H] +   
               
               
                   
               
             
            
               
                 70 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 186 [M + H − C 4 H 8 ] +   
               
               
                   
                 tert-butyl 1-(hydrazinecarbonyl)-3- 
                   
               
               
                   
                 azabicyclo[3.1.0]hexane-3-carboxylate 
                   
               
               
                   
               
               
                 71 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 158 
               
               
                   
               
               
                   
                 2-oxopiperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 72 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 268 [M + Na] +   
               
               
                   
               
               
                   
                 (R)-tert-butyl 2-(hydrazinecarbonyl) 
                   
               
               
                   
                 morpholine-4-carboxylate 
                   
               
               
                   
               
               
                 73 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 316 [M + Na] +   
               
               
                   
               
               
                   
                 tert-butyl 2-(hydrazinecarbonyl) 
                   
               
               
                   
                 thiomorpholine-4-carboxylate 1,1-dioxide 
               
               
                   
               
            
           
         
       
     
     Intermediate 74: Benzyl 3-fluoro-3-(hydrazinecarbonyl)piperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a stirred solution of hydrazine hydrate (0.155 mL, 7.11 mmol), 1-((benzyloxy)carbonyl)-3-fluoropiperidine-3-carboxylic acid (2.00 g, 7.11 mmol) and DIPEA (5.02 ml, 28.4 mmol) in DCM (70 mL) was added tripropyl phosphonic anhydride (50% v/v solution in EtOAc, 6.38 mL, 14.2 mmol) dropwise. The mixture was stirred at room temperature for 12 h. The reaction mixture was quenched by adding saturated aqueous sodium bicarbonate. The mixture was stirred for 5 min, the organic layer was separated, dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated to afford benzyl 3-fluoro-3-(hydrazinecarbonyl)piperidine-1-carboxylate. LCMS (C 14 H 18 FN 3 O 3 ) (ES, m/z): 296 [M+H] + . Intermediate 75 and Intermediate 76: tert-butyl (2R,5S or 2S,5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidine-1-carboxylate and tert-butyl (2S,5R or 2R,5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     A solution of rac,cis-tert-butyl 5-(hydrazinecarbonyl)-2-methylpiperidine-1-carboxylate (Intermediate 66) (5.00 g, 19.4 mmol) in DCM (7 mL) was added AcOH (0.556 ml, 9.72 mmol). The mixture was stirred at room temperature. To the mixture was added 2-((((2,4-dimethoxybenzyl) imino) methylene) amino)-5-fluoro-4-methoxybenzonitrile (Intermediate 37) (6.63 g, 19.4 mmol). The mixture was stirred for 60 h. The mixture was filtered, and the filtrate was loaded directly onto a silica gel column and purified with 0-100% EtOAc in hexane as eluent to provide the racemic tert-butyl (2R,5S and 2S,5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidine-1-carboxylate. The racemic mixture was resolved by chiral SFC (Chiral Technologies AD-H 50×250 mm column, with 35% EtOH as cosolvent) to afford tert-butyl (2R,5S or 2S,5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidine-1-carboxylate (Intermediate 75, first eluting peak) and tert-butyl (2S,5R or 2R,5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidine-1-carboxylate (Intermediate 76, second eluting peak). The intermediates in the following Table 10 were prepared in a manner similar to Intermediate 75 and Intermediate 76, from the appropriate intermediates and starting materials. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 10 
               
               
                   
               
               
                   
                 Structure 
                 SFC 
                 Observed m/z 
               
               
                 Intermediate 
                 Name 
                 Conditions 
                 [M + H] +   
               
               
                   
               
             
            
               
                 77 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AD-H 21 × 25 mm column with 50% (IPA w/ 0.2% DIPA modifier) as co-solvent 
                 565 
               
               
                   
               
               
                   
                 tert-butyl (1R,5R or 1S,5S)-1-(5-((2,4- 
                   
                   
               
               
                   
                 dimethoxybenzyl)amino)-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3- 
                   
                   
               
               
                   
                 azabicyclo[3.1.0]hexane-3-carboxylate 
                   
                   
               
               
                   
               
               
                 78 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AD-H 21 × 250 mm column with 50% (IPA w/ 0.2% DIPA modifier) as co-solvent 
                 565 
               
               
                   
               
               
                   
                 tert-butyl (1S,5S or 1R,5R)-1-(5-((2,4- 
                   
                   
               
               
                   
                 dimethoxybenzyl)amino)-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3- 
                   
                   
               
               
                   
                 azabicyclo[3.1.0]hexane-3-carboxylate 
               
               
                   
               
            
           
         
       
     
     Intermediates 79-81: tert-butyl (3S,5R or 3R,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidine-1-carboxylate and tert-butyl (3R,5S or 3S,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidine-1-carboxylate and tert-butyl (3R,5R and 3S,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     Intermediates 79-81 were prepared from Intermediate 37 and Intermediate 58 in a manner similar to that used for the preparation of Intermediate 75 and Intermediate 76. The crude residue was purified by silica gel chromatography with 0-100% EtOAc in hexane as eluent to afford tert-butyl (3S,5R and 3R,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidine-1-carboxylate (first eluting peak, mixture of Intermediate 79 and Intermediate 80) and tert-butyl (3R,5R and 3S,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidine-1-carboxylate (second eluting peak, Intermediate 81). For Intermediate 81: LCMS (C 29 H 34 F 2 N 6 O 5 ) (ES, m/z): 585 [M+H] + . The mixture of Intermediate 79 and Intermediate 80 was resolved by chiral SFC (Chiral Technologies AD-H 50×250 mm column with 35% MeOH as cosolvent) to afford tert-butyl (3S,5R or 3R,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidine-1-carboxylate (Intermediate 79, first eluting peak) and tert-butyl (3R,5S or 3S,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidine-1-carboxylate (Intermediate 80, second eluting peak). For Intermediate 79: LCMS (C 29 H 34 F 2 N 6 O 5 ) (ES, m/z): 585 [M+H] + . For Intermediate 80: LCMS (C 29 H 34 F 2 N 6 O 5 ) (ES, m/z): 585 [M+H] + . 
     Intermediate 82: (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     Step 1: (R)-tert-butyl 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a 40 mL vial was added (R)-tert-butyl 3-(hydrazinecarbonyl)piperidine-1-carboxylate (Intermediate 54) (596 mg, 2.45 mmol), DCM (7 mL) and AcOH (0.070 ml, 1.2 mmol). To the mixture was added 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5-fluoro-4-methoxybenzonitrile (Intermediate 37) (836 mg, 2.45 mmol). The mixture was stirred for 16 h. The solution was loaded onto a silica gel column and purified with 0-80% EtOAc in hexane as eluent to afford (R)-tert-butyl 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate LCMS (C 29 H 35 FN 6 O 5 ) (ES, m/z) [M+H] + : 567. 
     Step 2: (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added (R)-tert-butyl 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate (1.40 g, 2.47 mmol) and formic acid (4 mL). The solution was stirred for 16 h. The mixture was diluted with DCM (50 mL) and washed with 2 M aqueous potassium carbonate (75 mL). The mixture was extracted with additional DCM (50 mL). The combined organic layers were dried over sodium sulfate, filtered, and the solvents of the filtrate were evaporated to afford (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 82). LCMS (C 24 H 27 FN 6 O 3 ) (ES, m/z) [M+H] + : 467. 
     The intermediates in the following Table 11 were synthesized in a manner similar to that used in the preparation of Intermediate 82 from the appropriate intermediates and starting materials. For the synthesis of Intermediate 89, the deprotection step in formic acid (step 2) was not necessary. 
     
       
         
           
               
               
               
             
               
                 TABLE 11 
               
               
                   
               
               
                   
                   
                 Ob- 
               
               
                   
                   
                 served 
               
               
                   
                   
                 m/z 
               
               
                 Inter- 
                 Structure 
                 [M + 
               
               
                 mediate 
                 Name 
                 H] +   
               
               
                   
               
             
            
               
                 83 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 2-(azepan-3-yl)-N-(2,4-dimethoxybenzyl)-9-fluoro-8- 
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 84 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 2-(azepan-3-yl)-N-(2,4-dimethoxybenzyl)-9-fluoro-7- 
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 85 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(5- 
                   
               
               
                   
                 methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin- 
                   
               
               
                   
                 5-amine 
                   
               
               
                   
               
               
                 86 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(4- 
                   
               
               
                   
                 methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin- 
                   
               
               
                   
                 5-amine 
                   
               
               
                   
               
               
                 87 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 (R)-N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2- 
                   
               
               
                   
                 (morpholin-2-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5- 
                   
               
               
                   
                 amine 
                   
               
               
                   
               
               
                 88 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 517 
               
               
                   
               
               
                   
                 2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8- 
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
               
               
                   
                 yl)thiomorpholine 1,1-dioxide 
                   
               
               
                   
               
               
                 89 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8- 
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin- 
                   
               
               
                   
                 2-one 
               
               
                   
               
            
           
         
       
     
     Intermediate 90 and Intermediate 91: (S or R)—N-(2,4-dimethoxybenzyl)-9-fluoro-2-(3-fluoropiperidin-3-yl)-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R or S)—N-(2,4-dimethoxybenzyl)-9-fluoro-2-(3-fluoropiperidin-3-yl)-7-methoxy-[ 1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     Step 1: rac-benzyl 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[ 1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-fluoropiperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a stirred solution of rac-benzyl 3-fluoro-3-(hydrazinecarbonyl)piperidine-1-carboxylate (Intermediate 74) (1.73 g, 5.86 mmol) in DCM (25 mL) was added AcOH (0.201 mL, 3.52 mmol). The mixture was stirred at room temperature for 10 min. To the mixture was added 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5-fluoro-3-methoxybenzonitrile (Intermediate 38) (2.00 g, 5.86 mmol). The mixture was stirred and heated at 40° C. for 16 h. The mixture was cooled to room temperature. The mixture was diluted with DCM (100 mL) and then washed with saturated aqueous sodium bicarbonate and brine. The organic layer was dried over anhydrous MgSO 4 , filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with EtOAc in isohexane as eluent to afford rac-benzyl 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-fluoropiperidine-1-carboxylate. LCMS (C 32 H 32 F 2 N 6 O 5 ) (ES, m/z): 619 [M+H] + . 
     Step 2: (S or R)—N-(2,4-dimethoxybenzyl)-9-fluoro-2-(3-fluoropiperidin-3-yl)-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R or S)—N-(2,4-dimethoxybenzyl)-9-fluoro-2-(3-fluoropiperidin-3-yl)-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     A 200 mL round bottom flask was charged rac-benzyl 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-fluoropiperidine-1-carboxylate (2.00 g, 3.23 mmol), 10% Pd/C (800 mg, 3.23 mmol), and MeOH (50 mL). The mixture was stirred under an atmosphere of hydrogen for 16 h. The mixture was filtered through Celite® (diatomaceous earth). and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 0-8% MeOH in DCM (with 0.2% NH 4 OH) as eluent to afford a racemic mixture that was resolved by chiral SFC separation (Chiral Technologies, IC 20×250 mm column with 50% (EtOH with 0.2% DEA modifier) as cosolvent) to afford (S or R)—N-(2,4-dimethoxybenzyl)-9-fluoro-2-(3-fluoropiperidin-3-yl)-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (first eluting peak, Intermediate 90) and (R or S)—N-(2,4-dimethoxybenzyl)-9-fluoro-2-(3-fluoropiperidin-3-yl)-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (second eluting peak, Intermediate 91). For Intermediate 90: LCMS (C 24 H 26 F 2 N 6 O 3 ) (ES, m/z): 485 [M+H] + . For Intermediate 91: LCMS (C 24 H 26 F 2 N 6 O 3 ) (ES, m/z): 485 [M+H] + . 
     Intermediate 92: (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-7-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     Step 1: tert-butyl (R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a solution of tert-butyl (R)-3-(hydrazinecarbonyl)piperidine-1-carboxylate (Intermediate 54) (1.52 g, 6.25 mmol) in DCM (25 mL) was added AcOH (0.201 mL, 3.52 mmol). The mixture was stirred at room temperature for 10 min. To this mixture was added 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5-fluoro-3-methoxybenzonitrile (Intermediate 38) (2.00 g, 5.86 mmol). The mixture was stirred for 16 h. The mixture was diluted with DCM (100 mL), washed with saturated aqueous sodium bicarbonate and brine. The organic layer was dried over anhydrous MgSO 4 , the solids were removed by filtration, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with EtOAc in isohexane as eluent to afford tert-butyl (R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate. LCMS (C 29 H 35 FN 6 O 5 ) (ES, m/z): 567 [M+H] + . 
     Step 2: (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-7-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     To a solution of tert-butyl (R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate (2.12 g, 3.74 mmol) in DCM (30 mL) was added 4 M HCl in dioxane (10 mL, 40.0 mmol). The mixture was stirred at room temperature for 2 h. The solvents were evaporated. The residue was purified by silica gel chromatography with 0-8% MeOH in DCM (with 0.2% NH 4 OH) as eluent to afford (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-7-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 92). LCMS (C 24 H 27 FN 6 O 3 ) (ES, m/z): 467 [M+H] + . 
     The intermediates in the following Table 12 were prepared in a manner similar to that used in the preparation of Intermediate 92 from the appropriate intermediates and starting materials. 
     
       
         
           
               
               
               
             
               
                 TABLE 12 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Inter- 
                 Structure 
                 m/z 
               
               
                 mediate 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 93 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 453 
               
               
                   
               
               
                   
                 (R)-N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2- 
                   
               
               
                   
                 (pyrrolidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5- 
                   
               
               
                   
                 amine 
                   
               
               
                   
               
               
                 94 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 471 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-2-(3- 
                   
               
               
                   
                 fluoropyrrolidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-5-amine 
               
               
                   
               
            
           
         
       
     
     The intermediates in the following Table 12A were prepared in a manner similar to that used in step 2 of the preparation of Intermediate 92 from the appropriate intermediates and starting materials. 
     
       
         
           
               
               
               
             
               
                 TABLE 12A 
               
               
                   
               
               
                 Inter- 
                   
                 Observed  
               
               
                 me- 
                 Structure 
                 m/z 
               
               
                 diate 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 95 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2- 
                   
               
               
                   
                 ((3R,6S or 3S,6R)-6-methylpiperidin-3-yl)- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 96 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2- 
                   
               
               
                   
                 ((3S,6R or 3R,6S)-6-methylpiperidin-3-yl)- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 97 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 465 
               
               
                   
               
               
                   
                 2-((1R,5R or 1S,5S)-3-azabicyclo[3.1.0]hexan- 
                   
               
               
                   
                 1-yl)-N-(2,4-dimethoxybenzyl)-9-fluoro-8- 
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin- 
                   
               
               
                   
                 5-amine 
                   
               
               
                   
               
               
                 98 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 465 
               
               
                   
               
               
                   
                 2-((1S,5S or 1R,5R)-3-azabicyclo[3.1.0]hexan- 
                   
               
               
                   
                 1-yl)-N-(2,4-dimethoxybenzyl)-9-fluoro-8- 
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin- 
                   
               
               
                   
                 5-amine 
                   
               
               
                   
               
               
                 99 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 485 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3R,5S or 
                   
               
               
                   
                 3S,5R)-5-fluoropiperidin-3-yl)-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 100 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 485 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3S,5R or 
                   
               
               
                   
                 3R,5S)-5-fluoropiperidin-3-yl)-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 101 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 485 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3R,5R and 
                   
               
               
                   
                 3S,5S)-5-fluoropiperidin-3-yl)-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 102 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 485 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3R,5S and 
                   
               
               
                   
                 3S,5R)-5-fluoropiperidin-3-yl)-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 103 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 485 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3R,5R and 
                   
               
               
                   
                 3S,5S)-5-fluoropiperidin-3-yl)-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
               
               
                   
               
            
           
         
       
     
     Intermediate 104: rac-N′-(2-amino-6-fluoro-8-methoxyquinazolin-4-yl)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide 
     Step 1: 2-amino-6-fluoro-8-methoxyquinazolin-4-ol 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 2-amino-5-fluoro-3-methoxybenzoic acid (278 mg, 1.50 mmol) in EtOH (1.5 mL) was added cyanamide (158 mg, 3.75 mmol) and hydrochloric acid (325 μL, 1.95 mmol) (6 M, aqueous). The mixture was heated at reflux for 16 h. The mixture was cooled. The precipitate was collected by filtration and dried under high vacuum to afford 2-amino-6-fluoro-8-methoxyquinazolin-4-ol. LCMS (C 9 H 8 FN 3 O 2 ) (ES, m/z): 210 [M+H] + . 
     Step 2: 6-fluoro-8-methoxy-4-(1H-1,2,4-triazol-1-yl)quinazolin-2-amine 
     
       
         
         
             
             
         
       
     
     POCl 3  (295 μL, 3.16 mmol) was added dropwise over 15 min to a stirred mixture of 1,2,4-triazole (524 mg, 7.59 mmol), 2-amino-6-fluoro-8-methoxyquinazolin-4-ol (264.7 mg, 1.265 mmol), and DIPEA (553 μL, 3.16 mmol) in acetonitrile (5 mL) at room temperature. The mixture was stirred and heated at 40° C. for 3 h and then at room temperature for 16 h. The mixture was filtered through Celite® (diatomaceous earth). washing with acetonitrile and diethyl ether to afford 6-fluoro-8-methoxy-4-(1H-1,2,4-triazol-1-yl)quinazolin-2-amine. LCMS (C 11 H 9 FN 6 O) (ES, m/z): 261 [M+H] + . 
     Step 3: rac-N′-(2-amino-6-fluoro-8-methoxyquinazolin-4-yl)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide 
     
       
         
         
             
             
         
       
     
     A 20 mL reaction vial was charged with 6-fluoro-8-methoxy-4-(1H-1,2,4-triazol-1-yl)quinazolin-2-amine (41.1 mg, 0.158 mmol), (R and S)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide Intermediate 67) (38.8 mg, 0.174 mmol), THF (1 mL) and DIPEA (138 μl, 0.790 mmol). The mixture was stirred and heated at 50° C. for 4 h. The mixture was diluted with ethyl acetate (10 mL) and washed with saturated aqueous sodium bicarbonate (20 mL). The organic layer was dried over anhydrous MgSO 4 , filtered, and the solvents of the filtrate were evaporated to afford rac-N′-(2-amino-6-fluoro-8-methoxyquinazolin-4-yl)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide. LCMS (C 19 H 23  FN 8 O 2 ) (ES, m/z): 415 [M+H] + . 
     The intermediates in the following Table 13 were prepared from the appropriate starting materials in a manner similar to Intermediate 104, with the exception that the enantiopure hydrazide, Intermediate 67b, was used. 
     
       
         
           
               
               
               
             
               
                 TABLE 13 
               
               
                   
               
               
                   
                 Structure 
                 Observed 
               
               
                 Intermediate 
                 Name 
                 m/z [M + H] +   
               
               
                   
               
             
            
               
                 105 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 403 
               
               
                   
               
               
                   
                 (R os S)-N′-(2-amino-6,7-difluoroquinazolin-4-yl)-1-(1- 
                   
               
               
                   
                 methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 106 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 385 
               
               
                   
               
               
                   
                 (R or S)-N′-(2-amino-6-fluoroquinazolin-4-yl)-1-(1- 
                   
               
               
                   
                 methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 107 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 403 
               
               
                   
               
               
                   
                 (R or S)-N′-(2-amino-6,8-difluoroquinazolin-4-yl)-1-(1- 
                   
               
               
                   
                 methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 108 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 403 
               
               
                   
               
               
                   
                 (R or S)-N′-(2-amino-5,6-difluoroquinazolin-4-yl)-1-(1- 
                   
               
               
                   
                 methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 109 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 401 
               
               
                   
               
               
                   
                 (R or S)-N′-(2-amino-6-chloroquinazolin-4-yl)-1-(1- 
                   
               
               
                   
                 methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 110 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 415 
               
               
                   
               
               
                   
                 (R or S)-N′-(2-amino-6-chloro-8-methylquinazolin-4- 
                   
               
               
                   
                 yl)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3- 
                   
               
               
                   
                 carbohydrazide 
                   
               
               
                   
               
               
                 111 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 431 
               
               
                   
               
               
                   
                 (R or S)-N′-(2-amino-6-chloro-8-methoxyquinazolin-4- 
                   
               
               
                   
                 yl)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3- 
                   
               
               
                   
                 carbohydrazide 
                   
               
               
                   
               
               
                 112 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 397 
               
               
                   
               
               
                   
                 (R or S)-N′-(2-amino-6-methoxyquinazolin-4-yl)-1-(1- 
                   
               
               
                   
                 methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide 
               
               
                   
               
            
           
         
       
     
     Intermediates 113-116: 1-(4-((2R or 2S,5S or 5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2S or 2R,5R or 5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2S or 2R,5S or 5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2R or 2S,5R or 5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     Step 1: 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-6-ethyl-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)piperidin-2-one 
     
       
         
         
             
             
         
       
     
     To a solution of 6-ethyl-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)-2-oxopiperidine-3-carbohydrazide (Intermediate 64) (270 mg, 0.835 mmol) in dioxane (7 mL) was added AcOH (0.024 mL, 0.42 mmol). The mixture was stirred at room temperature for 30 min. To this mixture was added 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5-fluoro-4-methoxybenzonitrile (Intermediate 37) (285 mg, 0.835 mmol). The mixture was stirred at room temperature for 3 days. The mixture was filtered, and the filtrate was purified by silica gel chromatography with 0-100% 3:1 EtOAc:EtOH in hexanes as eluent to afford 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-6-ethyl-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)piperidin-2-one. LCMS (C 33 H 39 FN 8 O 5 ) (ES, m/z): 647 [M+H] + . 
     Step 2: 1-(4-((2R or 2S,5S or 5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2S or 2R,5R or 55)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2S or 2R,5S or 5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2R or 2S,5R or 55)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To the solution of 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-6-ethyl-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)piperidin-2-one (320 mg, 0.495 mmol) in THF (4.9 mL) was added borane in THF (1.0 M, 2.47 mL, 2.47 mmol). The mixture was stirred at room temperature for 24 h. The reaction mixture was quenched with MeOH, and then the solvents were evaporated. The resulting residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm with MeCN/water (with 0.1% TFA) as eluent) to afford two racemic mixtures of the corresponding diastereomers. Each racemate was resolved by chiral SFC. 
     The first eluting racemate was resolved by chiral SFC separation (Chiral Technologies, AS-H, 21×250 mm column with 50% (IPA+0.2% DIPA) as co-solvent) to afford 1-(4-((2R or 2S,5S or 5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (first eluting peak) and 1-(4-((2S or 2R,5R or 55)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (second eluting) corresponding to Intermediate 113 and Intermediate 114, respectively. For Intermediate 113: LCMS (C 33 H 41 FN 8 O 4 ) (ES, m/z): 634 [M+H] + . For Intermediate 114: LCMS (C 33 H 41 FN 8 O 4 ) (ES, m/z): 634 [M+H] + . 
     The second eluting racemate was resolved by chiral SFC separation (AS-H, 21×250 mm column with 50% (IPA+0.2% DIPA) as co-solvent) to afford 1-(4-((2S or 2R,5S or 5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (first eluting peak) and 1-(4-((2R or 2S,5R or 5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (second eluting peak), corresponding to Intermediate 115 and Intermediate 116, respectively. For Intermediate 115: LCMS (C 33 H 41 FN 8 O 4 ) (ES, m/z): 634 [M+H] + . For Intermediate 116: LCMS (C 33 H 41 FN 8 O 4 ) (ES, m/z): 634 [M+H] + . 
     Intermediate 117: mixture of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(3-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(5-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     Step 1: mixture of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(5-methyl-1-trityl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(3-methyl-1-trityl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     A 20 mL microwave vial was charged with (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 82) (500 mg, 1.07 mmol) and THF (6.7 mL). To the mixture was added the mixture of 4-bromo-5-methyl-1-trityl-1H-pyrazole and 4-bromo-3-methyl-1-trityl-1H-pyrazole (Intermediate 16) (865 mg, 2.14 mmol), followed by tBuXPhos-Pd G3 (412 mg, 4.29 mmol) and sodium tert-butoxide (412 mg, 4.29 mmol). Nitrogen was bubbled through the mixture for 10 min. The mixture was stirred and heated at 90° C. for 16 h. The mixture was cooled to room temperature. To the mixture was added Celite® (diatomaceous earth) and saturated aqueous NH 4 Cl. The mixture was stirred vigorously for 5 min. The mixture was filtered through Celite® (diatomaceous earth) topped with anhydrous MgSO 4  and washed with DCM. The solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 0-20% MeOH in DCM to afford the mixture of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(5-methyl-1-trityl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(3-methyl-1-trityl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. LCMS (C 47 H 45 FN 8 O 3 ) (ES, m/z): 789 [M+H] + . 
     Step 2: Mixture of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(3-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(5-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     To a stirred solution of the mixture of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(3-methyl-1-trityl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(5-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (565 mg, 0.716 mmol) in MeOH (7.2 mL) was added a 4 M solution of HCl in dioxane (1.79 mL, 7.16 mmol). The mixture was stirred at room temperature for 1 h. The solvents were evaporated, and the residue was dissolved in DCM (50 mL). To the mixture was added saturated aqueous sodium bicarbonate (50 mL). The biphasic mixture was separated, and the aqueous layer was extracted with additional DCM (50 mL). The combined organic layers were dried over anhydrous MgSO 4 , filtered, and the solvents of the filtrate were evaporated to afford the mixture of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(3-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(5-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. LCMS (C 28 H 31 FN 8 O 3 ) (ES, m/z): 547 [M+H] + . 
     The intermediates in the following Table 14 were prepared in a manner similar to that used for the preparation of Intermediate 117, from the appropriate starting materials. 
     
       
         
           
               
               
               
             
               
                 TABLE 14 
               
               
                   
               
               
                   
                 Structure 
                 Observed 
               
               
                 Intermediate 
                 Name 
                 m/z [M + H] +   
               
               
                   
               
             
            
               
                 118 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 533 
               
               
                   
               
               
                   
                 (R)-2-(1-(1H-pyrazol-4-yl)piperidin-3-yl)-N-(2,4- 
                   
               
               
                   
                 dimethoxybenzyl)-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 119 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 583 
               
               
                   
               
               
                   
                 (R)-2-(1-(3-(difluoromethyl)-1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-N-(2,4-dimethoxybenzyl)-9-fluoro- 
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
               
               
                   
               
            
           
         
       
     
     Intermediate 120 and Intermediate 121: N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S or 3S, 6R)-6-methyl-1-(1-((1R,2R or 1S,2S)-2-((tetrahydro-2H-pyran-2-yl)oxy)cyclopentyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S or 3S, 6R)-6-methyl-1-(1-((1S,2S or 1R,2R)-2-((tetrahydro-2H-pyran-2-yl)oxy)cyclopentyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     To a reaction vial containing of solution of N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S or 3S,6R)-6-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 95) (150 mg, 0.312 mmol) in THF (4 mL) was added 4-bromo-1-(2-((tetrahydro-2H-pyran-2-yl)oxy)cyclopentyl)-1H-pyrazole (Intermediate 32) (177 mg, 0.562 mmol), followed by tBuXPhos-Pd G3 (124 mg, 0.156 mmol) and sodium tert-butoxide (105 mg, 1.09 mmol). The mixture was sparged with nitrogen for 10 min. The mixture was stirred and heated at 90° C. for 16 h. To the mixture was added additional 4-bromo-1-(2-((tetrahydro-2H-pyran-2-yl)oxy)cyclopentyl)-1H-pyrazole (Intermediate 32) (88.5 mg, 0.281 mmol), tBuXPhos-Pd G3 (62 mg, 0.078 mmol) and sodium tert-butoxide (52.5 mg, 0.547 mmol). The mixture was stirred and heated at 90° C. for 16 h. The mixture was purified by preparative silica gel TLC with 4% MeOH in DCM as eluent to afford a mixture of isomers. The mixture was resolved by chiral SFC separation (ID 21×250 mm column with 50% (MeOH w/ACN 1:1+0.2% DIPA) as co-solvent) to afford N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S or 3S,6R)-6-methyl-1-(1-((1R,2R or 1S,2S)-2-((tetrahydro-2H-pyran-2-yl)oxy)cyclopentyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (first eluting peak, Intermediate 120) and N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S or 3S, 6R)-6-methyl-1-(1-((1S,2S or 1R,2R)-2-((tetrahydro-2H-pyran-2-yl)oxy)cyclopentyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (fourth eluting peak, Intermediate 121), corresponding to Intermediate 120 and Intermediate 121, respectively. (NOTE: peaks 2 and 3 had poor separation). For Intermediate 120: LCMS (C 34 H 47 FN 8 O 5 ) (ES, m/z): 715 [M+H] + . For Intermediate 121: LCMS (C 38 H 47 FN 8 O 5 ) (ES, m/z): 715 [M+H] + . 
     Intermediate 122 and Intermediate 123: 1-(4-((3R,5S and 3R,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((3R,5R and 3S,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     To a stirred mixture of sodium tert-butoxide (300 mg, 3.12 mmol), 1-(4-bromo-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 4) (274 mg, 1.25 mmol) and N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 85) (300 mg, 0.624 mmol) in THF (2 mL) was added tBuXPhos-Pd G3 (149 mg, 0.187 mmol) under a nitrogen atmosphere in a glove box. The mixture was stirred and heated at 100° C. for 14 h. The mixture was purified by preparative silica gel TLC with 10% MeOH in DCM as eluent to afford the two diastereomers: 1-(4-((3R,5S and 3R,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((3R,5R and 3S,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol, corresponding to Intermediate 122 and Intermediate 123, respectively. For Intermediate 122, LCMS (C 32 H 39 FN 8 O 4 ) (ES, m/z): 619 [M+H] + . For Intermediate 123, LCMS (C 32 H 39 FN 8 O 4 ) (ES, m/z): 619 [M+H] + . 
     Intermediate 124: (R)-5-(4-(3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)pentan-2-one 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of sodium tert-butoxide (66.5 mg, 0.692 mmol), (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 82) (89.0 mg, 0.190 mmol), 2-(4-bromo-1H-pyrazol-1-yl)-1-methylcyclobutanol (Intermediate 29) (40.0 mg, 0.173 mmol) in THF (2 mL) was added tBuXPhos-Pd G3 (41.3 mg, 0.0520 mmol). The mixture was stirred and heated at 80° C. for 12 h. The mixture was cooled, diluted with EtOAc (10 mL), and washed with water (10 mL). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by preparative silica gel TLC with EtOAc as eluent, affording the ring-opened product (R)-5-(4-(3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)pentan-2-one. LCMS (C 32 H 37 FN 8 O 4 ) (ES, m/z): 617 [M+H] + . 
     Intermediate 125: 2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)cyclobutan-1-one 
     Step 1: N-(2,4-dimethoxybenzyl)-2-((3R)-1-(1-(2,2-dimethoxycyclobutyl)-1H-pyrazol-4-yl)piperidin-3-yl)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of sodium tert-butoxide (177 mg, 1.84 mmol), (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 82) (236 mg, 0.506 mmol), 4-bromo-1-(2,2-dimethoxycyclobutyl)-1H-pyrazole (Intermediate 30) (120 mg, 0.460 mmol) in THF (4 mL) was added tBuXPhos-Pd G3 (110 mg, 0.138 mmol). The mixture was stirred and heated at 80° C. for 12 h under nitrogen. The mixture was cooled, diluted with EtOAc (10 mL), and then washed with water (10 mL). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified silica gel chromatography with 0-100% EtOAc in petroleum ether as eluent to afford N-(2,4-dimethoxybenzyl)-2-((3R)-1-(1-(2,2-dimethoxycyclobutyl)-1H-pyrazol-4-yl)piperidin-3-yl)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. LCMS (C 33 H 39 FN 8 O 5 ) (ES, m/z): 647 [M+H] + . 
     Step 2: 2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)cyclobutan-1-one 
     
       
         
         
             
             
         
       
     
     A mixture of N-(2,4-dimethoxybenzyl)-2-((3R)-1-(1-(2,2-dimethoxycyclobutyl)-1H-pyrazol-4-yl)piperidin-3-yl)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (130 mg, 0.201 mmol) and formic acid (2 mL) was stirred and heated at 40° C. for 15 h. The mixture was cooled and adjusted to pH=8 with saturated aqueous sodium bicarbonate. The mixture was extracted with DCM (3×10 mL). The combined organic layers were dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by preparative silica gel TLC with EtOAc as eluent to afford 2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)cyclobutan-1-one. LCMS (C 22 H 23 FN 8 O 2 ) (ES, m/z): 451 [M+H] + . 
     Intermediate 126 and Intermediate 127: rac-(1R or 1S,3R or 3S)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol and rac-(1S or 1R,3S or 3R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol 
     Step 1: 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)cyclohexan-1-ol 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 3-hydroxycyclohexanecarbohydrazide (Intermediate 65) (0.556 g, 3.52 mmol) in DCM (30 mL) was added AcOH (0.084 mL, 1.5 mmol). To the solution was added 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5-fluoro-4-methoxybenzonitrile (Intermediate 37) (1.00 g, 2.93 mmol). The mixture was stirred and heated at 35° C. for 10 h. The mixture was concentrated. The resulting residue was purified by silica gel chromatography with 10-50% EtOAc in petroleum ether as eluent to afford 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)cyclohexanol. LCMS (C 25 H 28 FN 5 O 4 ) (ES, m/z) [M+H] + : 482. 
     Step 2: 3-(5-((2,4-Dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)cyclohexan-1-one 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)cyclohexanol (780 mg, 1.62 mmol) in DCM (10 mL) was added DMP (1.37 g, 3.24 mmol) at 0° C. The mixture was warmed to room temperature and stirred for 3 h. The mixture was quenched with saturated aqueous sodium bicarbonate (5 mL), and the mixture was filtered. The filtrate was extracted with EtOAc (3×10 mL). The combined organic layers were washed with brine (30 mL), dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 10-50% EtOAc in petroleum ether to afford 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)cyclohexanone. LCMS (C 25 H 26 FN 5 O 4 ) (ES, m/z) [M+H] + : 480. 
     Step 3: 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 4-bromo-1-ethyl-1H-pyrazole (621 mg, 3.55 mmol) in THF (3 mL) was added n-butyllithium (1.42 mL, 3.55 mmol, 2.5 M in hexane) at −78° C. The mixture was stirred at −78° C. for 20 min. To the mixture was added a solution of 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)cyclohexanone (340 mg, 0.709 mmol) in THF (3 mL) at −78° C., and the mixture was stirred at this temperature for 1 h. The mixture was quenched with saturated aqueous NH 4 Cl (5 mL) and extracted with EtOAc (3×10 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 10-50% EtOAc in petroleum ether as eluent to afford 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl). LCMS (C 34 H 34 FN 7 O 4 ) (ES, m/z) [M+H] + : 576. 
     Step 4: rac-(1R or 1S,3R or 35)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol and rac-(15 or 1R,3S or 3R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol 
     
       
         
         
             
             
         
       
     
     To a solution of 3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexanol (180 mg, 0.313 mmol) in DCM (4 mL) and water (2 mL) was added DDQ (106 mg, 0.469 mmol) portionwise at 0° C. The mixture was stirred at 0° C. for 30 min. The mixture was diluted with DCM (10 mL) and was washed with Na 2 SO 3  (2 M aqueous solution, 5 mL) and brine (2×10 mL). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by reversed-phase HPLC (Waters XBridge C18 OBD Prep Column, 19 mm×100 mm with MeCN/water (w/10 mM NH 4 HCO 3  modifier) as eluent) to afford two diastereomers rac-(1R or 1S,3R or 3S)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol and rac-(15 or 1R,3S or 3R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol, corresponding to Intermediate 126 and Intermediate 127, respectively. For Intermediate 126: LCMS (C 21 H 24 FN 7 O 2 ) (ES, m/z) [M+H] + : 426. For Intermediate 127: LCMS (C 21 H 24 FN 7 O 2 ) (ES, m/z) [M+H] + : 426. 
     Intermediate 128: (2S,3S and 2R,3R)-3-(4-bromo-1H-pyrazol-1-yl)butan-2-ol 
     
       
         
         
             
             
         
       
     
     To a mixture of 4-bromo-1H-pyrazole (2.00 g, 13.6 mmol) and cesium carbonate (13.3 g, 40.8 mmol) in MeCN (20 mL) was added cis-2,3-dimethyloxirane (2.38 ml, 27.2 mmol). The mixture was stirred and heated at 80° C. for 16 h. The mixture was cooled to room temperature and the solids were removed by filtration. The filtrate was concentrated, and the residue was diluted with DCM and washed with water and brine solution. The organic layer was dried over anhydrous sodium sulfate. The residue was purified by silica gel chromatography with 0-100% EtOAc in hexanes as eluent to afford (2S,3S and 2R,3R)-3-(4-bromo-1H-pyrazol-1-yl)butan-2-ol. LCMS (C 7 H 11 BrN 2 O) (ES, m/z) [M+H] + : 219, 221. 
     The intermediates in the following Table 15 were prepared from the appropriate starting materials in a manner similar to Intermediate 128. 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 15 
               
               
                   
                   
               
               
                   
                   
                 Structure 
                 Observed 
               
               
                   
                 Intermediate 
                 Name 
                 m/z [M + H] +   
               
               
                   
                   
               
             
            
               
                   
                 129 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 219, 221 
               
               
                   
                   
               
               
                   
                   
                 racemic, anti-3-(4-bromo- 
                   
               
               
                   
                   
                 1H-pyrazol-1-yl)butan-2-ol 
                   
               
               
                   
                   
               
               
                   
                 130 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 233, 235 
               
               
                   
                   
               
               
                   
                   
                 racemic, syn-3-(4-bromo-3-methyl- 
                   
               
               
                   
                   
                 1H-pyrazol-1-yl)butan-2-ol 
                   
               
               
                   
                   
               
               
                   
                 131 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 233, 235 
               
               
                   
                   
               
               
                   
                   
                 racemic-syn-3-(4-bromo-5-methyl- 
                   
               
               
                   
                   
                 1H-pyrazol-1-yl)butan-2-ol 
                   
               
               
                   
                   
               
               
                   
                 132 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 186 
               
               
                   
                   
               
               
                   
                   
                 racemic-syn-3-(4-nitro-1H- 
                   
               
               
                   
                   
                 pyrazol-1-yl)butan-2-ol 
                   
               
               
                   
                   
               
               
                   
                 133 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 202 
               
               
                   
                   
               
               
                   
                   
                 rac-2-methyl-3-(4-nitro-1H-pyrazol- 
                   
               
               
                   
                   
                 1-yl)propane-1,2-diol 
                   
               
               
                   
                   
               
               
                   
                 134 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 200 
               
               
                   
                   
               
               
                   
                   
                 2-methyl-1-(5-methyl-4-nitro- 
                   
               
               
                   
                   
                 1H-pyrazol-1-yl)propan-2-ol 
                   
               
               
                   
                   
               
               
                   
                 135 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 200 
               
               
                   
                   
               
               
                   
                   
                 2-methyl-1-(3-methyl-4-nitro- 
                   
               
               
                   
                   
                 1H-pyrazol-1-yl)propan-2-ol 
               
               
                   
                   
               
            
           
         
       
     
     Intermediate 136: ethyl 3-(4-bromo-1H-pyrazol-1-yl)propanoate 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 4-bromo-1H-pyrazole (0.500 g, 3.40 mmol) in DMF (10 mL) was added K 2 CO 3  (1.18 g, 8.50 mmol) and ethyl 3-bromopropanoate (0.924 g, 5.10 mmol). The mixture was stirred and heated at 60° C. for 8 h. The mixture was diluted with water (30 mL), filtered, and the filtrate was extracted with EtOAc (2×30 mL). The organic layers were dried over anhydrous sodium sulfate, filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel column chromatography with 3-25% EtOAc in petroleum ether as eluent to afford ethyl 3-(4-bromo-1H-pyrazol-1-yl)propanoate. LCMS (C 8 H 11 BrN 2 O 2 ) (ES, m/z) [M+H] + : 247, 249. 
     The intermediates in the following Table 16 were prepared from the appropriate pyrazole and alkyl halide or mesylate in a manner similar to that used in the preparation of Intermediate 136. 
     
       
         
           
               
               
               
             
               
                 TABLE 16 
               
               
                   
               
               
                   
                 Structure 
                 Observed 
               
               
                 Intermediate 
                 Name 
                 m/z [M + H] +   
               
               
                   
               
             
            
               
                 137 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 231, 233 
               
               
                   
               
               
                   
                 3-(4-bromo-1H-pyrazol- 
                   
               
               
                   
                 1-yl)-3-methylbutan-2-one 
                   
               
               
                   
               
               
                 138 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 245, 247 
               
               
                   
               
               
                   
                 3-(4-bromo-3-methyl-1H-pyrazol- 
                   
               
               
                   
                 1-yl)-3-methylbutan-2-one 
                   
               
               
                   
               
               
                 139 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 261, 263 
               
               
                   
               
               
                   
                 methyl 2-(4-bromo-3-methyl- 
                   
               
               
                   
                 1H-pyrazol-1-yl)-2-methylpropanoate 
                   
               
               
                   
               
               
                 140 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 261, 263 
               
               
                   
               
               
                   
                 methyl 2-(4-bromo-5-methyl- 
                   
               
               
                   
                 1H-pyrazol-1-yl)-2-methylpropanoate 
                   
               
               
                   
               
               
                 141 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 203, 205 
               
               
                   
               
               
                   
                 methyl 2-(4-bromo-5-methyl-1H- 
                   
               
               
                   
                 pyrazol-1-yl)-2-methylpropanoate 
                   
               
               
                   
               
               
                 142 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 307 [M + Na] +   
               
               
                   
               
               
                   
                 tert-butyl (2-methyl-1-(4-nitro- 
                   
               
               
                   
                 1H-pyrazol-1-yl)propan-2-yl)carbamate 
                   
               
               
                   
               
               
                 143 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 198 
               
               
                   
               
               
                   
                 3-methyl-3-(4-nitro-1H-pyrazol- 
                   
               
               
                   
                 1-yl)butan-2-one 
                   
               
               
                   
               
               
                 144 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 — 
               
               
                   
               
               
                   
                 diethyl 2-methyl-2-(4-nitro-1H- 
                   
               
               
                   
                 pyrazol-1-yl)malonate 
               
               
                   
               
            
           
         
       
     
     Intermediate 145: rac-1-(4-bromo-1H-pyrazol-1-yl)-2-methylbutan-2-ol 
     
       
         
         
             
             
         
       
     
     To a solution of 1-(4-bromo-1H-pyrazol-1-yl)propan-2-one (Intermediate 141) (2.00 g, 9.85 mmol) in Et 2 O (22 mL) was added a 3 M solution of ethylmagnesium bromide (9.85 mL, 29.6 mmol) dropwise at 0° C. under a nitrogen atmosphere. The solution was stirred at 0° C. for 1 h, then warmed to room temperature and stirred for 15 h. The mixture was quenched with saturated aqueous ammonium chloride (50 mL), diluted with EtOAc (50 mL) and water (50 mL). The aqueous layer was extracted with EtOAc (3×100 mL). The combined organic layers were washed with brine (2×100 mL), dried over anhydrous Na 2 SO 4 , filtered and the solvents were evaporated. The resulting residue was purified by reversed phase HPLC (MeCN/water with 0.05% TFA) to afford rac-1-(4-bromo-1H-pyrazol-1-yl)-2-methylbutan-2-ol. LCMS (C 8 H 13 BrN 2 O) (ES, m/z) [M+H] + : 233, 235. 
     Intermediate 146: 1-((5-methyl-2-phenyl-1,3-dioxan-5-yl)methyl)-4-nitro-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a solution of (5-methyl-2-phenyl-1,3-dioxan-5-yl)methanol (4.00 g, 19.2 mmol) in THF (20 mL) was added 4-nitro-1H-pyrazole (2.61 g, 23.1 mmol), triphenylphosphine (10.1 g, 38.4 mmol). To the mixture was slowly added DIAD (5.83 g, 28.8 mmol) in DCM (20 mL). The mixture was stirred at room temperature for 15 h. The solvents were evaporated. The residue was purified by silica gel chromatography with 0-30% EtOAc in petroleum ether to afford 1-((5-methyl-2-phenyl-1,3-dioxan-5-yl)methyl)-4-nitro-1H-pyrazole. LCMS (C 15 H 17 N 3 O 4 ) (ES, m/z) [M+H] + : 304. 
     The intermediate in the following Table 17 was prepared from the appropriate pyrazole and alcohol in a manner similar to that described for the synthesis of Intermediate 146. 
     
       
         
           
               
               
               
             
               
                 TABLE 17 
               
               
                   
               
               
                   
                 Structure 
                 Observed 
               
               
                 Intermediate 
                 Name 
                 m/z [M + H] +   
               
               
                   
               
             
            
               
                 147 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 558 [2M + Na] +   
               
               
                   
               
               
                   
                 4-nitro-1-((1-((tetrahydro-2H-pyran-2- 
                   
               
               
                   
                 yl)oxy)cyclopropyl)methyl)-1H-pyrazole 
               
               
                   
               
            
           
         
       
     
     Intermediate 148: rac-3-(4-bromo-3-methyl-1H-pyrazol-1-yl)-3-methylbutan-2-ol 
     
       
         
         
             
             
         
       
     
     To a suspension of 3-(4-bromo-3-methyl-1H-pyrazol-1-yl)-3-methylbutan-2-one (Intermediate 138) (1.27 g, 5.18 mmol) in EtOH (25.9 ml) was added sodium borohydride (0.588 g, 15.5 mmol). The mixture was stirred for 16 h. The mixture was diluted with EtOAc (50 mL), washed with water (50 mL) and aqueous potassium hydroxide (1 M, 20 mL). The organic layer was dried over anhydrous MgSO 4 , filtered, and the solvent of the filtrate was evaporated to afford 3-(4-bromo-3-methyl-1H-pyrazol-1-yl)-3-methylbutan-2-ol, which was taken forward without further purification. LCMS (C 9 H 15 BrN 2 O) (ES, m/z) [M+H] + : 247, 249. 
     The intermediates in the following Table 18 were prepared from the appropriate ketone or ester containing pyrazole in a manner similar to that described in the preparation of Intermediate 148. 
     
       
         
           
               
               
               
             
               
                 TABLE 18 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Inter- 
                 Structure 
                 m/z 
               
               
                 mediate 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 149 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 233, 235 
               
               
                   
               
               
                   
                 2-(4-bromo-3-methyl-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-1-ol 
                   
               
               
                   
               
               
                 150 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 233, 235 
               
               
                   
               
               
                   
                 2-(4-bromo-5-methyl-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-1-ol 
                   
               
               
                   
               
               
                 151 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 200 
               
               
                   
               
               
                   
                 rac-3-methyl-3-(4-nitro-1H-pyrazol-1-yl)butan-2-ol 
                   
               
               
                   
               
               
                 152 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 202 
               
               
                   
               
               
                   
                 4-nitro-1-((1-((tetrahydro-2H-pyran-2- 
                   
               
               
                   
                 yl)oxy)cyclopropyl)methyl)-1H-pyrazole 
               
               
                   
               
            
           
         
       
     
     Intermediate 153: 4-(4-bromo-1H-pyrazol-1-yl)-2-methylbutan-2-ol 
     
       
         
         
             
             
         
       
     
     To a solution of ethyl 3-(4-bromo-1H-pyrazol-1-yl)propanoate (100 mg, 0.405 mmol) (Intermediate 136) in THF (4 mL) was added a 3 M solution of MeMgBr in diethyl ether (1.35 mL, 4.05 mmol) at 15° C. under a nitrogen atmosphere. The mixture was stirred at 15° C. for 1 h. The mixture was cooled at 0° C., diluted with water (5 mL), extracted with EtOAc (2×5 mL). The organic layer was dried over anhydrous sodium sulfate, filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by preparative silica gel TLC with 25% EtOAc in petroleum ether as eluent to afford 4-(4-bromo-1H-pyrazol-1-yl)-2-methylbutan-2-ol. LCMS (C 8 H 13 BrN 2 O) (ES, m/z) [M+H] + : 233, 235. 
     Intermediate 154: 4-bromo-1-((racemic, anti)-3-((rac-tetrahydro-2H-pyran-2-yl)oxy)butan-2-yl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a solution of Intermediate 129 (9.40 g, 42.9 mmol) in DCM (100 mL) was added 3,4-dihydro-2H-pyran (19.6 mL, 215 mmol) and PPTS (10.8 g, 42.9 mmol). The mixture was stirred at room temperature for 4 h. The mixture was diluted with DCM (15 mL), washed with saturated aqueous NaHCO 3  and brine solution. The organic layer was dried over anhydrous sodium sulfate, filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-100% EtOAc in hexanes as eluent to afford rac-4-bromo-1-((2S,3R)-3-((tetrahydro-2H-pyran-2-yl)oxy)butan-2-yl)-1H-pyrazole. LCMS (C 12 H 19 BrN 2 O 2 ) (ES, m/z) [M+H] + : 303, 305. 
     The intermediate in the following Table 19 was prepared from the appropriate starting materials in a manner similar to that described for the synthesis of Intermediate 154. 
     
       
         
           
               
               
               
             
               
                 TABLE 19 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Inter- 
                 Structure 
                 m/z 
               
               
                 mediate 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 155 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 303, 305 
               
               
                   
               
               
                   
                 rac-4-bromo-1-(2-methyl-1-((tetrahydro-2H-pyran-2- 
                   
               
               
                   
                 yl)oxy)propan-2-yl)-1H-pyrazole 
               
               
                   
               
            
           
         
       
     
     Intermediate 156: rac-3-(4-amino-1H-pyrazol-1-yl)-3-methylbutan-2-ol 
     
       
         
         
             
             
         
       
     
     A 100 mL round-bottom flask was charged with 10% Pd/C (200 mg, 0.188 mmol), 3-methyl-3-(4-nitro-1H-pyrazol-1-yl)butan-2-ol (Intermediate 151) (750 mg, 3.76 mmol), and EtOH (31.4 mL). The mixture was degassed by evacuating and backfilling with nitrogen three times. The mixture was then evacuated and refilled with hydrogen from a balloon. The mixture was stirred for 3 h. The mixture was filtered through Celite, and the solvents of the filtrate were evaporated to afford 3-(4-amino-1H-pyrazol-1-yl)-3-methylbutan-2-ol. LCMS (C 8 H 15 N 3 O) (ES, m/z) [M+H] + : 170. 
     The intermediates in the following Table 20 were prepared from the appropriate nitro-pyrazole in a manner similar to that described for the preparation of Intermediate 156. 
     
       
         
           
               
               
               
             
               
                 TABLE 20 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Inter- 
                 Structure 
                 m/z 
               
               
                 mediate 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 157 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 255 
               
               
                   
               
               
                   
                 tert-butyl (1-(4-amino-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-2-yl)carbamate 
                   
               
               
                   
               
               
                 158 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 — 
               
               
                   
               
               
                   
                 2-(4-amino-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol 
                   
               
               
                   
               
               
                 159 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 172 
               
               
                   
               
               
                   
                 rac-3-(4-amino-1H-pyrazol-1-yl)-2-methylpropane-1,2- 
                   
               
               
                   
                 diol 
                   
               
               
                   
               
               
                 160 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 156 
               
               
                   
               
               
                   
                 racemic, syn--3-(4-amino-1H-pyrazol-1-yl)butan-2-ol 
                   
               
               
                   
               
               
                 161 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 238 
               
               
                   
               
               
                   
                 rac-1-((1-((tetrahydro-2H-pyran-2- 
                   
               
               
                   
                 yl)oxy)cyclopropyl)methyl)-1H-pyrazol-4-amine 
                   
               
               
                   
               
               
                 162 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 274 
               
               
                   
               
               
                   
                 1-((5-methyl-2-phenyl-1,3-dioxan-5-yl)methyl)-1H- 
                   
               
               
                   
                 pyrazol-4-amine 
                   
               
               
                   
               
               
                 163 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 170 
               
               
                   
               
               
                   
                 1-(4-amino-5-methyl-1H-pyrazol-1-yl)-2-methylpropan- 
                   
               
               
                   
                 2-ol 
                   
               
               
                   
               
               
                 164 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 170 
               
               
                   
               
               
                   
                 1-(4-amino-3-methyl-1H-pyrazol-1-yl)-2-methylpropan- 
                   
               
               
                   
                 2-ol 
               
               
                   
               
            
           
         
       
     
     Intermediate 165: tert-butyl (1-(4-((2S,5R and 2R,5S)-5-(hydrazinecarbonyl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-yl)carbamate 
     
       
         
         
             
             
         
       
     
     Step 1: methyl (3R,6S and 3S,6R)-1-(1-(2-((tert-butoxycarbonyl)amino)-2-methylpropyl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carboxylate 
     To a 20 mL vial was added tert-butyl (1-(4-amino-1H-pyrazol-1-yl)-2-methylpropan-2-yl)carbamate (Intermediate 157) (85.0 mg, 0.334 mmol), sodium triacetoxyborohydride (106 mg, 0.501 mmol), and DCE (1.5 mL). The mixture was stirred. To the mixture was added methyl 2-methylene-5-oxohexanoate (0.063 ml, 0.40 mmol). After 20 minutes, to the mixture was added 1 M aqueous KOH (3 mL), water (3 mL), and DCM (3 mL). The organic layer was collected with a phase separator. The solvents were evaporated. To the resulting residue was dissolved in MeOH (1 mL), and water (0.4 mL). The mixture was stirred at room temperature for 72 h. To the mixture was added water (20 mL). The mixture was extracted with DCM (2×20 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered, and the solvents were evaporated. The resulting residue was purified by silica gel chromatography with 0-100% EtOAc in hexanes as eluent to afford methyl (3R,6S and 3S,6R)-1-(1-(2-((tert-butoxycarbonyl)amino)-2-methylpropyl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carboxylate. LCMS (C 24 H 34 N 4 O 4 ) (ES, m/z) [M+H] + : 395. 
     Step 2: tert-butyl (1-(4-((2S,5R and 2R,5S)-5-(hydrazinecarbonyl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-yl)carbamate 
     To a 20 mL vial was added methyl (3R,6S and 3S,6R)-1-(1-(2-((tert-butoxycarbonyl)amino)-2-methylpropyl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carboxylate (99.1 mg, 0.251 mmol), EtOH (1 mL), and hydrazine hydrate (0.175 ml, 3.77 mmol). The mixture was heated at 90° C. for 16 h. The solvents were evaporated to afford tert-butyl (1-(4-((2S,5R and 2R,5S)-5-(hydrazinecarbonyl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-yl)carbamate. LCMS (C 19 H34N 6 O 3 ) (ES, m/z) [M+H] + : 395. 
     The intermediates in the following Table 21 were prepared from the appropriate amino-pyrazole in a manner similar to that described for the preparation of Intermediate 165. 
     
       
         
           
               
               
               
             
               
                 TABLE 21 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Inter- 
                 Structure 
                 m/z 
               
               
                 mediate 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 166 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 312 
               
               
                   
               
               
                   
                 1-(1-(1,3-dihydroxy-2-methylpropan-2-yl)-1H-pyrazol- 
                   
               
               
                   
                 4-yl)-6-methylpiperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 167 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 312 
               
               
                   
               
               
                   
                 1-(1-(2,3-dihydroxy-2-methylpropyl)-1H-pyrazol-4-yl)- 
                   
               
               
                   
                 6-methylpiperidine-3-carbohydrazide 
                   
               
               
                   
               
               
                 168 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 414 
               
               
                   
               
               
                   
                 6-methyl-1-(1-((5-methyl-2-phenyl-1,3-dioxan-5- 
                   
               
               
                   
                 yl)methyl)-1H-pyrazol-4-yl)piperidine-3- 
                   
               
               
                   
                 carbohydrazide 
                   
               
               
                   
               
               
                 169 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 378 
               
               
                   
               
               
                   
                 6-methyl-1-(1-((1-((tetrahydro-2H-pyran-2- 
                   
               
               
                   
                 yl)oxy)cyclopropyl)methyl)-1H-pyrazol-4-yl)piperidine- 
                   
               
               
                   
                 3-carbohydrazide 
                   
               
               
                   
               
               
                 170 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 224 
               
               
                   
               
               
                   
                 (3R,6S and 3S,6R)-6-methyl-1-(1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidine-3-carbohydrazide 
               
               
                   
               
            
           
         
       
     
     Intermediate 171: 1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carbohydrazide 
     
       
         
         
             
             
         
       
     
     Step 1: methyl 4-methyl-2-methylene-5-oxopentanoate 
     A solution of methyl 2-(bromomethyl)acrylate (6.04 ml, 50.3 mmol) and DMAP (6.76 g, 55.3 mmol) in DCM (201 mL) was stirred at room temperature for 30 minutes. To the mixture were added propionaldehyde (5.41 ml, 75 mmol) and L-proline (5.79 g, 50.3 mmol). The mixture was stirred at 23° C. for 48 hours. The mixture was washed with water (200 mL), 1 M aqueous HCl (100 mL), and brine (100 mL). The organic layer was dried over anhydrous MgSO 4 , filtered, and the solvents were evaporated. The resulting residue was purified by silica gel chromatography with 0-2.5% MeOH in DCM as eluent to afford methyl 4-methyl-2-methylene-5-oxopentanoate. 
     Step 2: methyl (3R,5R and 3S,5S)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carboxylate 
     1-(4-amino-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 163) (1.43 g, 8.45 mmol) was dissolved in MeOH (25.6 mL). To the mixture was added sodium triacetoxyborohydride (6.51 g, 30.7 mmol), followed by methyl 4-methyl-2-methylene-5-oxopentanoate (1.20 mL, 7.68 mmol). The mixture was stirred for 5 min (until the sodium triacetoxyborohydride went into solution). The mixture was quenched with 1 M aqueous sodium hydroxide (30.7 mL, 30.7 mmol) and allowed to stir for 48 h. The MeOH was evaporated, and to the mixture was added DCM (30 mL). The layers were separated and the DCM layer was dried with anhydrous MgSO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 0-10% MeOH in DCM to afford methyl (3R,5R and 3S,5S)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carboxylate. LCMS (C 16 H 27 N 3 O 3 ) (ES, m/z) [M+H] + : 310. 
     Step 3: Mixture of methyl (3S,5R and 3R,5S)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carboxylate and methyl (3R,5R and 3S,5S)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carboxylate 
     Methyl (3R,5R and 3S,5S)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carboxylate (1.39 g, 4.49 mmol) was stirred in MeOH (35.9 ml) with potassium tert-butoxide (1.01 g, 8.98 mmol) for 18 h at 60° C. The mixture was cooled to room temperature, and the solvents were evaporated. The resulting residue was dissolved in DCM and quenched with saturated aqueous NH 4 Cl. The layers were separated using a Biotage Isolute® phase separator and the DCM layer was concentrated. The resulting residue was purified by silica gel chromatography with 0-10% MeOH in DCM to afford a mixture of methyl (3S,5R and 3R,5S)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carboxylate and methyl (3R,5R and 3S,5S)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carboxylate. LCMS (C 16 H 27 N 3 O 3 ) (ES, m/z) [M+H] + : 310. 
     Step 4: (3R,5S and 3S,5R)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carbohydrazide and (3R,5R and 3S,5S)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carbohydrazide 
     To a solution of the product from step 3 (1.18 g, 3.81 mmol) in ethanol (15.3 mL) was added hydrazine hydrate (1.87 mL, 38.1 mmol). The mixture was stirred and heated at 80° C. for 16 h. The mixture was cooled to room temperature and the solvents were evaporated to afford (3R,5S and 3S,5R)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carbohydrazide and (3R,5R and 3S,5S)-1-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)-5-methylpiperidine-3-carbohydrazide LCMS (C 15 H 27 N 5 O 2 ) (ES, m/z) [M+H] + : 310. 
     Intermediate 172 and Intermediate 173: N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,5S and 3S,5R)-5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3S,5S and 3R,5R)-5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 85) (2.52 g, 5.24 mmol) was subjected to chiral SFC separation (Phenomenex Lux-3 21×250 mm column with 20% MeOH (w/0.1% NH 4 OH) as cosolvent) to afford rac-N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,5S)-5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (combination of peaks 2 and 3) and rac-N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3S,5S)-5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (combination of peaks 1 and 4). LCMS (C 25 H 29 FN 6 O 3 ) (ES, m/z) [M+H] + : 481. 
     Intermediate 174 and Intermediate 175: N-(2,4-dimethoxybenzyl)-7,9-difluoro-2-((3R,5S and 3S,5R)-5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and N-(2,4-dimethoxybenzyl)-7,9-difluoro-2-((3S,5S and 3R,5R)-5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     Step 1: tert-butyl (3R,5S and 3S,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidine-1-carboxylate and tert-butyl (3S,5S and 3R,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidine-1-carboxylate 
     To a 100 mL round bottom flask was added tert-butyl 3-(hydrazinecarbonyl)-5-methylpiperidine-1-carboxylate (Intermediate 56) (1.56 g, 6.07 mmol), 1,4-dioxane (20 mL), and acetic acid (0.174 mL, 3.04 mmol). The mixture was stirred. To this stirring mixture was added 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-3,5-difluorobenzonitrile (Intermediate 41) (2.00 g, 6.07 mmol). The mixture was stirred at 75° C. for 16 h. The mixture was purified by silica gel chromatography with 0-50% EtOAc in hexanes as eluent to afford tert-butyl (3R,5S and 3S,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidine-1-carboxylate (first eluting) and tert-butyl (3S,5S and 3R,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidine-1-carboxylate (second eluting). LCMS (C 29 H 34 F 2 N 6 O 4 ) (ES, m/z) [M+H] + : 569. 
     Step 2: N-(2,4-dimethoxybenzyl)-7,9-difluoro-2-((3R,5S and 3S,5R)-5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and N-(2,4-dimethoxybenzyl)-7,9-difluoro-2-((3S,5S and 3R,5R)-5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     tert-butyl (3R,5S and 3S,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidine-1-carboxylate and tert-butyl (3S,5S and 3R,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidine-1-carboxylate from Step 1 were converted to N-(2,4-dimethoxybenzyl)-7,9-difluoro-2-((3R,5S and 3S,5R)-5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 174) and N-(2,4-dimethoxybenzyl)-7,9-difluoro-2-((3S,5S and 3R,5R)-5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 175) (LCMS (C 24 H 26 F 2 N 6 O 2 ) (ES, m/z) [M+H] + : 469) with formic acid in a manner similar to the synthesis of Intermediate 82. 
     The intermediates in the following Table 22 were prepared in a similar manner to that described for the synthesis of Intermediate 174 from the appropriate hydrazide and carbodiimide. 
     
       
         
           
               
               
               
             
               
                 TABLE 22 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Inter- 
                 Structure 
                 m/z 
               
               
                 mediate 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 176 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-7-methoxy-2- 
                   
               
               
                   
                 ((3R,5S and 3S,5R)-5-methylpiperidin-3-yl)- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 177 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 547 
               
               
                   
               
               
                   
                 N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2- 
                   
               
               
                   
                 ((3R,6S and 3S,6R)-6-methyl-1-(1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5- 
                   
               
               
                   
                 amine 
               
               
                   
               
            
           
         
       
     
     Intermediate 178: (R)-8-(difluoromethoxy)-N-(2,4-dimethoxybenzyl)-9-fluoro-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     Step 1: 2-amino-4-(difluoromethoxy)-5-fluorobenzonitrile 
     
       
         
         
             
             
         
       
     
     To a mixture of 2-bromo-5-(difluoromethoxy)-4-fluoroaniline (1.40 g, 5.47 mmol) and zinc cyanide (1.28 g, 10.9 mmol) in NMP (4 mL) was added bis(tri-tert-butylphosphine)palladium(0) (0.699 g, 1.37 mmol). The mixture was stirred and heated at 160° C. under nitrogen for 1 h in a microwave reactor. The mixture was cooled to room temperature. To the mixture was added brine (60 mL), and the mixture was extracted with petroleum ether:ethyl acetate (3:1) (3×25 mL), the combined organic layers were dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 25% EtOAc in petroleum ether to afford 2-amino-4-(difluoromethoxy)-5-fluorobenzonitrile. 
     Step 2: methyl (2-cyano-5-(difluoromethoxy)-4-fluorophenyl)carbamate 
     
       
         
         
             
             
         
       
     
     A solution of 2-amino-4-(difluoromethoxy)-5-fluorobenzonitrile (500 mg, 2.47 mmol) in methyl carbonochloridate (3.04 g, 32.2 mmol) was stirred and heated at 75° C. under a nitrogen atmosphere for 15 h. The mixture was cooled, diluted with water (10 mL), extracted with EtOAc (3×20 mL), dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography to afford methyl (2-cyano-5-(difluoromethoxy)-4-fluorophenyl)carbamate. 
     Step 3: (R)-tert-butyl-3-(8-(difluoromethoxy)-9-fluoro-5-hydroxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a solution of methyl (2-cyano-5-(difluoromethoxy)-4-fluorophenyl)carbamate (400 mg, 1.537 mmol) in NMP (4 mL) was added (R)-tert-butyl 3-(hydrazinecarbonyl)piperidine-1-carboxylate (411 mg, 1.691 mmol) (Intermediate 54). The mixture was stirred and heated at 170° C. for 30 min. The mixture was cooled, diluted with water (30 mL) and extracted with EtOAc (3×30 mL). The combined organic extracts were dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-30% EtOAc in hexanes as eluent to afford (R)-tert-butyl-3-(8-(difluoromethoxy)-9-fluoro-5-hydroxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate. LCMS (C 24 H 22 F 3 N 5 O 4 ) (ES, m/z) [M+H] + : 454. 
     Step 4: tert-butyl-3-(8-(difluoromethoxy)-5-((2,4-dimethoxybenzyl)amino)-9-fluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a solution of tert-butyl-3-(8-(difluoromethoxy)-9-fluoro-5-hydroxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate (1.00 g, 2.21 mmol) in MeCN (10 mL) was added DBU (0.831 mL, 5.51 mmol), PyBroP (1.34 g, 2.87 mmol) and (2,4-dimethoxyphenyl)methanamine (0.553 g, 3.31 mmol) at 90° C. under a nitrogen atmosphere. The mixture was stirred at 90° C. for 12 h. The solvents were evaporated. The resulting residue was purified by silica gel chromatography with 0-50% EtOAc in hexanes as eluent to afford tert-butyl-3-(8-(difluoromethoxy)-5-((2,4-dimethoxybenzyl)amino)-9-fluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate. LCMS (C 29 H 33 F 3 N 6 O 5 ) (ES, m/z) [M+H] + : 603. 
     Step 5: (R)-8-(difluoromethoxy)-N-(2,4-dimethoxybenzyl)-9-fluoro-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     To a solution of tert-butyl-3-(8-(difluoromethoxy)-5-((2,4-dimethoxybenzyl)amino)-9-fluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidine-1-carboxylate (700 mg, 1.16 mmol) in DCM (7 mL) was added TFA (0.7 mL) at 15° C. under a nitrogen atmosphere. The mixture was stirred at 15° C. for 2 h. The mixture was cooled, diluted with NaHCO 3  (15 mL), extracted with DCM (3×20 mL), dried over anhydrous Na 2 SO 4 , and the solvents were evaporated. The resulting residue was purified by silica gel chromatography with 0-50% EtOAc in petroleum ether as eluent to afford (R)-8-(difluoromethoxy)-N-(2,4-dimethoxybenzyl)-9-fluoro-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. LCMS (C 24 H 25 F 3 N 6 O 3 ) (ES, m/z) [M+H] + : 503. 
     Example 1: (R)-1-(3-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-1,2,4-triazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     A 5 mL microwave vial was charged with (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 82) (100 mg, 0.214 mmol), tBuXPhos-Pd G3 (68.1 mg, 0.086 mmol) and sodium tert-butoxide (82 mg, 0.86 mmol). To the mixture was added 1-(3-bromo-1H-1,2,4-triazol-1-yl)-2-methylpropan-2-ol (Intermediate 2) (94 mg, 0.429 mmol) in THF (1.4 mL). The mixture was sparged with nitrogen for 10 min. The mixture was stirred and heated at 90° C. for 16 h. The mixture was cooled to room temperature, and the solids were removed by filtration and washed with DCM. The solvents of the filtrate were evaporated. To the resulting residue was added TFA (0.5 mL). The mixture was stirred and heated at 50° C. for 3 h. The mixture was cooled to room temperature, and the solvents were evaporated. The residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm MeCN/H 2 O with 0.1% TFA as eluent) to afford (R)-1-(3-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-1,2,4-triazol-1-yl)-2-methylpropan-2-ol. LCMS (C 21 H 26 FN 9 O 2 ) (ES, m/z): 458 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 8.17 (s, 1H), 7.89 (d, J=10.9 Hz, 1H), 7.81 (s, 2H), 7.19 (d, J=7.9 Hz, 1H), 4.31 (d, J=12.8 Hz, 1H), 3.97 (s, 4H), 3.91 (s, 2H), 3.15 (ddt, J=10.9, 6.7, 3.4 Hz, 1H), 3.11-3.04 (m, 1H), 2.86 (td, J=12.5, 2.7 Hz, 1H), 2.23 (d, J=12.1 Hz, 1H), 1.92-1.77 (m, 2H), 1.75-1.63 (m, 1H), 1.10 (d, J=2.4 Hz, 6H). 
     The example compounds of the invention in the following Table 15 were prepared in a manner similar to that described in Example 1, from the appropriate starting aryl halide and amine intermediates. 
     
       
         
           
               
               
               
             
               
                 TABLE 15 
               
               
                   
               
               
                   
                 Structure 
                 Observed 
               
               
                 Example 
                 Name 
                 m/z [M + H] +   
               
               
                   
               
             
            
               
                  2 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 397 
               
               
                   
               
               
                   
                 (R)-9-fluoro-8-methoxy-2-(1-(1-methyl-1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                  3 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 439 
               
               
                   
               
               
                   
                 (R)-2-(1-(1-(tert-butyl)-1H-pyrazol-4-yl)piperidin-3-yl)-9- 
                   
               
               
                   
                 fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                  4 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 425 
               
               
                   
               
               
                   
                 (R)-9-fluoro-2-(1-(1-isopropyl-1H-pyrazol-4-yl)piperidin-3-yl)- 
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                  5 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 465 
               
               
                   
               
               
                   
                 (R)-9-fluoro-8-methoxy-2-(1-(1-(2,2,2-trifluoroethyl)-1H- 
                   
               
               
                   
                 pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5- 
                   
               
               
                   
                 amine 
                   
               
               
                   
               
               
                  6 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 398 
               
               
                   
               
               
                   
                 (R)-9-fluoro-8-methoxy-2-(1-(1-methyl-1H-1,2,3-triazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                  7 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 455 
               
               
                   
               
               
                   
                 (R)-1-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-2-ol 
                   
               
               
                   
               
               
                  8 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 (R)-1-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-5-methyl-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-2-ol 
                   
               
               
                   
               
               
                  9 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 (R)-1-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-2-ol 
                   
               
               
                   
               
               
                 10 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 467 
               
               
                   
               
               
                   
                 (R)-1-((4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1- 
                   
               
               
                   
                 yl)methyl)cyclobutan-1-ol 
                   
               
               
                   
               
               
                 11 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 (R)-2-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-1-ol 
                   
               
               
                   
               
               
                 12 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 (from Intermediate 26) 
                   
               
               
                   
                 (S or R)-3-(4-((R)-3-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H- 
                   
               
               
                   
                 pyrazol-1-yl)-2-methylbutan-2-ol 
                   
               
               
                   
               
               
                 13 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 (from Intermediate 27) 
                   
               
               
                   
                 (R or S)-3-(4-((R)-3-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H- 
                   
               
               
                   
                 pyrazol-1-yl)-2-methylbutan-2-ol 
                   
               
               
                   
               
               
                 14 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 467 
               
               
                   
               
               
                   
                 (1s,3s)-3-(4-((R)-3-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H- 
                   
               
               
                   
                 pyrazol-1-yl)-1-methylcyclobutan-1-ol 
                   
               
               
                   
               
               
                 15 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 467 
               
               
                   
                 (R)-9-fluoro-8-methoxy-2-(1-(1-((3-methyloxetan-3- 
                   
               
               
                   
                 yl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 16 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 (R)-9-fluoro-8-methoxy-2-(1-(5-methyl-1-(tetrahydro-2H- 
                   
               
               
                   
                 pyran-4-yl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 17 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 (R)-9-fluoro-8-methoxy-2-(1-(3-methyl-1-(tetrahydro-2H- 
                   
               
               
                   
                 pyran-4-yl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 18 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 517 
               
               
                   
               
               
                   
                 (R)-2-(1-(5-(difluoromethyl)-1-(tetrahydro-2H-pyran-4-yl)-1H- 
                   
               
               
                   
                 pyrazol-4-yl)piperidin-3-yl)-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 19 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 517 
               
               
                   
               
               
                   
                 (R)-2-(1-(3-(difluoromethyl)-1-(tetrahydro-2H-pyran-4-yl)-1H- 
                   
               
               
                   
                 pyrazol-4-yl)piperidin-3-yl)-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 20 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 441 
               
               
                   
               
               
                   
                 (R)-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-1-ethyl-1H-pyrazol-3- 
                   
               
               
                   
                 yl)methanol 
                   
               
               
                   
               
               
                 21 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 495 
               
               
                   
               
               
                   
                 (R)-1-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-3-cyclopropyl-1H-pyrazol-1- 
                   
               
               
                   
                 yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 22 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 460 
               
               
                   
               
               
                   
                 (R)-2-(1-(6-(difluoromethoxy)pyridin-3-yl)piperidin-3-yl)-9- 
                   
               
               
                   
                 fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 23 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 452 
               
               
                   
               
               
                   
                 (R)-9-fluoro-2-(1-(6-isopropoxypyridin-3-yl)piperidin-3-yl)-8- 
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 24 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 474 
               
               
                   
               
               
                   
                 (R)-2-(1-(6-(difluoromethoxy)-5-methylpyridin-3-yl)piperidin- 
                   
               
               
                   
                 3-yl)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5- 
                   
               
               
                   
                 amine 
                   
               
               
                   
               
               
                 25 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 474 
               
               
                   
               
               
                   
                 (R)-5-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-1-(difluoromethyl)-3- 
                   
               
               
                   
                 methylpyridin-2(1H)-one 
                   
               
               
                   
               
               
                 26 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 424 
               
               
                   
               
               
                   
                 (R)-9-fluoro-8-methoxy-2-(1-(6-methoxypyridin-3-yl)piperidin- 
                   
               
               
                   
                 3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 27 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 470 
               
               
                   
               
               
                   
                 (R)-1-(3-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-5-methyl-1H-1,2,4-triazol-1- 
                   
               
               
                   
                 yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 28 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 455 
               
               
                   
               
               
                   
                 (R)-1-(4-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-2-ol 
                   
               
               
                   
               
               
                 29 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 (R)-1-(4-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-5-methyl-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-2-ol 
                   
               
               
                   
               
               
                 30 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 (R)-1-(4-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-2-ol 
                   
               
               
                   
               
               
                 31 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 467 
               
               
                   
               
               
                   
                 (R)-1-((4-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1- 
                   
               
               
                   
                 yl)methyl)cyclobutan-1-ol 
                   
               
               
                   
               
               
                 32 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 455 
               
               
                   
               
               
                   
                 (R)-2-(4-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-1-ol 
                   
               
               
                   
               
               
                 33 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 460 
               
               
                   
               
               
                   
                 (R)-2-(1-(6-(difluoromethoxy)pyridin-3-yl)piperidin-3-yl)-9- 
                   
               
               
                   
                 fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 34 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 424 
               
               
                   
               
               
                   
                 (R)-9-fluoro-7-methoxy-2-(1-(6-methoxypyridin-3-yl)piperidin- 
                   
               
               
                   
                 3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 35 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 452 
               
               
                   
               
               
                   
                 (R)-9-fluoro-2-(1-(6-isopropoxypyridin-3-yl)piperidin-3-yl)-7- 
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 36 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 456 
               
               
                   
               
               
                   
                 (R)-1-(3-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-1H-1,2,4-triazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-2-ol 
                   
               
               
                   
               
               
                 37 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 470 
               
               
                   
               
               
                   
                 (R)-1-(3-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-5-methyl-1H-1,2,4-triazol-1- 
                   
               
               
                   
                 yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 38 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 453 
               
               
                   
               
               
                   
                 (R)-1-((4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)pyrrolidin-1-yl)-1H-pyrazol-1- 
                   
               
               
                   
                 yl)methyl)cyclobutan-1-ol 
                   
               
               
                   
               
               
                 39 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 rac-1-(4-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)azepan-1-yl)-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-2-ol 
               
               
                   
               
            
           
         
       
     
     Example 40: 1-((4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 
     Step 1: N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S or 3S,6R)-6-methyl-1-(1-((1-(((RS)-tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     To a reaction vial containing of solution of N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S or 3S,6R)-6-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 95) (600 mg, 1.25 mmol) in THF (12 ml) was added rac-4-bromo-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole (Intermediate 23) (590 mg, 1.87 mmol) followed by tBuXPhos-Pd G3 (298 mg, 0.375 mmol) and sodium tert-butoxide (420 mg, 4.37 mmol). Nitrogen was bubbled through the mixture for 10 min. The mixture was stirred and heated at 90° C. for 4 h. The mixture was cooled to room temperature. To the mixture was added additional tBuXPhos-Pd G3 (149 mg, 0.188 mmol) followed by sodium tert-butoxide (210 mg, 2.19 mmol). Nitrogen was bubbled through the mixture for an additional 10 min. The mixture was stirred and heated at 90° C. for 18 h. The mixture was cooled to room temperature, and then the solvents were evaporated. The residue was partitioned between DCM and water. The organic layer was washed with brine, dried over anhydrous MgSO 4 , and the solids were removed by filtration. The filtrate was concentrated. The resulting residue was purified by silica gel chromatography with 0-40% EtOAc:EtOH (3:1) in hexane as eluent. The obtained residue was further purified by preparative silica gel TLC with 4% MeOH in DCM as eluent to afford N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S or 3S,6R)-6-methyl-1-(1-((1-(((RS)-tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. LCMS (C 38 H 47 FN 8 O 5 ) (ES, m/z): 715 [M+H] + . 
     Step 2: 1-((4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 
     
       
         
         
             
             
         
       
     
     To a reaction vial was added N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S or 3S,6R)-6-methyl-1-(1-((1-(((RS)-tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (1.40 g, 2.22 mmol) and TFA (1.65 mL, 22.2 mmol). The mixture was stirred and heated at 60° C. for 1 h. The mixture was cooled to room temperature, and then the solvents were evaporated. The residue was purified by silica gel chromatography with 6% (7 M ammonia solution in MeOH) in DCM as eluent to afford 1-((4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol (Example 40). LCMS (C 24 H 29 FN 8 O 2 ) (ES, m/z): 481 [M+H] + .  1 H NMR (500 MHz, Methanol-d 4 ) δ 7.87 (d, J=10.9 Hz, 1H), 7.35 (s, 1H), 7.27 (s, 1H), 7.15 (d, J=7.6 Hz, 1H), 4.14 (s, 2H), 3.99 (s, 3H), 3.73 (d, J=4.9 Hz, 1H), 3.47 (d, J=9.2 Hz, 1H), 3.36-3.20 (m, 3H), 2.11 (d, J=7.9 Hz, 3H), 2.02 (p, J=10.7, 9.6 Hz, 2H), 1.89-1.69 (m, 2H), 1.55 (dq, J=19.0, 9.6 Hz, 1H), 1.37-1.20 (m, 2H), 1.12 (d, J=6.6 Hz, 3H), 0.96-0.82 (m, 2H). 
     The example compounds of the invention in the following Table 16 were prepared in a manner similar to that described for the preparation of Example 40 from the appropriate starting aryl halide and Intermediate 95. 
     
       
         
           
               
               
               
             
               
                 TABLE 16 
               
               
                   
               
               
                   
                 Structure 
                 Observed m/z 
               
               
                 Example 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 41 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 483 
               
               
                   
               
               
                   
                 1-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1- 
                   
               
               
                   
                 yl)-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 42 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 483 
               
               
                   
               
               
                   
                 1-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1- 
                   
               
               
                   
                 yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 43 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 497 
               
               
                   
               
               
                   
                 3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1- 
                   
               
               
                   
                 yl)-1H-pyrazol-1-yl)-2,3-dimethylbutan-2-ol 
                   
               
               
                   
               
               
                 44 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 481 
               
               
                   
               
               
                   
                 9-fluoro-8-methoxy-2-((3R,6S or 3S,6R)-6-methyl-1-(1- 
                   
               
               
                   
                 (tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)piperidin-3-yl)- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 45 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 495 
               
               
                   
               
               
                   
                 9-fluoro-8-methoxy-2-((3R,6S or 3S,6R)-6-methyl-1-(5- 
                   
               
               
                   
                 methyl-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 46 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 495 
               
               
                   
               
               
                   
                 9-fluoro-8-methoxy-2-((3R,6S or 3S,6R)-6-methyl-1-(3- 
                   
               
               
                   
                 methyl-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 47 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 499 
               
               
                   
               
               
                   
                 9-fluoro-2-((3R,6S or 3S,6R)-1-(1-((3-(fluoromethyl)oxetan- 
                   
               
               
                   
                 3-yl)methyl)-1H-pyrazol-4-yl)-6-methylpiperidin-3-yl)-8- 
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
               
               
                   
               
            
           
         
       
     
     Example 48 and Example 49: (R or S)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylbutan-2-ol and (S or R)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylbutan-2-ol 
     Step 1: (R or S)-3-(4-((2S,5R or 2R,5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylbutan-2-ol and (S or R)-3-(4-((2S,5R or 2R,5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylbutan-2-ol 
     
       
         
         
             
             
         
       
     
     Step 1 of the synthesis of Example 48 and Example 49 was conducted with Intermediate 95 and Intermediate 1 in a manner similar to that described in step 1 of the synthesis of Example 40. The resulting diastereomeric mixture was purified by SFC (Chiral Technologies AD-H 21×250 mm column with 55% (IPA+0.2% DIPA) as co-solvent), to afford peak 1 and peak 2 corresponding to (R or S)-3-(4-((2S,5R or 2R,5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylbutan-2-ol and (S or R)-3-(4-((2S,5R or 2R,5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylbutan-2-ol. For peak 1, LCMS (C 33 H 41 FN 8 O 4 ) (ES, m/z): 633 [M+H] + . For peak 2, LCMS (C 33 H 41 FN 8 O 4 ) (ES, m/z): 633 [M+H] + . 
     Step 2: (R or S)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylbutan-2-ol and (S or R)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylbutan-2-ol 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Step 2 of the synthesis of Example 48 and Example 49 was conducted in a manner similar to that described in step 2 of Example 40, where peak 1 was converted to (R or S)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylbutan-2-ol (Example 48) and peak 2 was converted to (S or R)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylbutan-2-ol (Example 49). 
     For Example 48: LCMS (C 24 H 31 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (400 MHz, Chloroform-d) δ 7.99 (d, J=10.7 Hz, 1H), 7.26 (s, 1H), 7.15 (d, J=7.6 Hz, 1H), 7.01 (s, 1H), 5.74 (s, 2H), 4.14 (s, 1H), 4.02 (m, 1H), 4.00 (s, 3H), 3.74 (m, 1H), 3.49 (s, 1H), 3.45 (dd, J=11.6, 3.8 Hz, 1H), 3.32 (dd, J=11.3, 4.0 Hz, 1H), 3.20 (t, J=11.3 Hz, 1H), 2.18-2.00 (m, 3H), 1.78 (d, J=9.9 Hz, 1H), 1.52 (d, J=6.9 Hz, 3H), 1.14 (s, 3H), 1.11 (d, J=6.7 Hz, 2H), 1.01 (s, 3H). 
     For Example 49: LCMS (C 24 H 31 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (400 MHz, Chloroform-d) δ 7.99 (d, J=10.8 Hz, 1H), 7.26 (s, 1H), 7.15 (d, J=7.6 Hz, 1H), 7.01 (s, 1H), 5.78 (s, 2H), 4.18 (s, 1H), 4.03 (m, 1H), 4.00 (s, 4H), 3.74 (m, 1H), 3.49 (s, 1H), 3.44 (dd, J=11.6, 3.9 Hz, 1H), 3.33 (dd, J=11.0, 4.2 Hz, 1H), 3.21 (t, J=11.3 Hz, 1H), 2.18-2.00 (m, 3H), 1.78 (d, J=9.7 Hz, 1H), 1.52 (d, J=6.9 Hz, 3H), 1.14 (s, 3H), 1.11 (m, J=6.9 Hz, 3H), 1.02 (s, 3H). 
     Example 50 and Example 51: 1-((4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol and 1-((4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-5-methyl-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 
     Step 1: N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3S,6R or 3R,6S)-6-methyl-1-(3-methyl-1-((1-(((RS)-tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3S,6R, or 3R,6S)-6-methyl-1-(5-methyl-1-((1-(((RS)-tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     To a reaction vial was added N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3S,6R, or 3R,6S)-6-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 96) (240 mg, 0.499 mmol, tBuXPhos-Pd G3 (119 mg, 0.150 mmol), rac-4-bromo-3-methyl-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole (Intermediate 31) (329 mg, 0.999 mmol), sodium tert-butoxide (288 mg, 3.00 mmol) and THF (5 mL). The mixture was sparged with nitrogen for 5 min. The mixture was stirred and heated at 100° C. for 19 h. The solvents were evaporated and the residue was purified by preparative silica gel TLC with 4% (7 M ammonia in MeOH) in DCM as eluent to afford a mixture of N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3S,6R or 3R,6S)-6-methyl-1-(3-methyl-1-((1-(((RS)-tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3S,6R or 3R,6S)-6-methyl-1-(5-methyl-1-((1-(((RS)-tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. 
     Step 2: 1-((4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol and 1-((4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-5-methyl-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 
     
       
         
         
             
             
         
       
     
     To the mixture of N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3S,6R or 3R,6S)-6-methyl-1-(3-methyl-1-((1-(((RS)-tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3S,6R or 3R,6S)-6-methyl-1-(5-methyl-1-((1-(((RS)-tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (12.0 mg, 0.0186 mmol) was added TFA (2 mL). The mixture was stirred and heated at 60° C. for 1 h. The mixture was cooled to room temperature. The mixture was concentrated and the residue was purified by preparative silica gel TLC with 4% (7 M ammonia in MeOH) in DCM as eluent followed by reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm MeCN/water w/0.1% TFA modifier as eluent) to afford 1-((4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol (Example 50) and 1-((4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-5-methyl-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol (Example 51). 
     For Example 50: LCMS (C 25 H 31 FN 8 O 2 ) (ES, m/z): 495 [M+H] + .  1 H NMR (500 MHz, Methanol-d 4 ) δ 8.02 (s, 1H), 7.95 (d, J=10.8 Hz, 1H), 7.26 (d, J=7.7 Hz, 1H), 4.22 (s, 2H), 4.18 (d, J=12.9 Hz, 1H), 4.03 (s, 3H), 3.91 (s, 2H), 3.74 (s, 1H), 3.57-3.43 (m, 1H), 2.46 (s, 3H), 2.36-2.26 (m, 1H), 2.22-2.12 (m, 3H), 2.04 (q, J=9.7 Hz, 3H), 1.84-1.73 (m, 1H), 1.68-1.58 (m, 1H), 1.27 (d, J=6.5 Hz, 3H). 
     For Example 51: LCMS (C 25 H 31 FN 8 O 2 ) (ES, m/z): 495 [M+H] + .  1 H NMR (500 MHz, Methanol-d 4 ) δ 7.98-7.91 (m, 1H), 7.82 (s, 1H), 7.26 (d, J=7.7 Hz, 1H), 4.27 (s, 2H), 4.03 (s, 3H), 3.96 (d, J=11.8 Hz, 2H), 3.76 (d, J=20.2 Hz, 1H), 2.61 (s, 2H), 2.45 (d, J=15.4 Hz, 2H), 2.40-2.29 (m, 2H), 2.26 (s, 1H), 2.15 (d, J=10.4 Hz, 3H), 2.11-1.97 (m, 3H), 1.89-1.74 (m, 1H), 1.75-1.58 (m, 1H), 1.28 (d, J=6.6 Hz, 3H). 
     The example compounds of the invention in the following Table 17 were prepared in a manner similar to that described for Example 50 and Example 51 from the appropriate starting aryl halide and Intermediate 96. 
     
       
         
           
               
               
               
             
               
                 TABLE 17 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Ex- 
                 Structure 
                 m/z 
               
               
                 ample 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 52 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 483 
               
               
                   
               
               
                   
                 1-(4-((2R,5S, or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin- 
                   
               
               
                   
                 1-yl)-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 53 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 483 
               
               
                   
               
               
                   
                 1-(4-((2R,5S, or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin- 
                   
               
               
                   
                 1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
               
               
                   
               
            
           
         
       
     
     Example 54: 1-((4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 
     Step 1: N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3R,5S or 3S,5R)-5-fluoro-1-(1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     To a reaction vial containing of solution of N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3R,5S or 3S,5R)-5-fluoropiperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 99) (80.0 mg, 0.165 mmol) in THF (1.5 mL) was added 4-bromo-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole (Intermediate 23) (83.0 mg, 0.260 mmol) followed by tBuXPhos-Pd G3 (39.3 mg, 0.0500 mmol) and sodium tert-butoxide (47.6 mg, 0.495 mmol). The mixture was flushed with nitrogen for 10 min. The mixture was stirred and heated at 90° C. for 2 h. The solvents were evaporated. The resulting residue was purified by silica gel chromatography with 0-40% EtOAc:EtOH (3:1) in hexanes as eluent to afford N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3R,5S or 3S,5R)-5-fluoro-1-(1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. LCMS (C 37 H 44 F 2 N 8 O 5 ) (ES, m/z): 719 [M+H] + . 
     Step 2: 1-((4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 
     
       
         
         
             
             
         
       
     
     A mixture of N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3R,5S or 3S,5R)-5-fluoro-1-(1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (105 mg, 0.146 mmol) in TFA (1.2 mL) was stirred and heated at 60° C. for 1 h. The mixture was concentrated. The residue was purified by preparative silica gel TLC with 5% (7 M ammonia in MeOH) in DCM as eluent to afford 1-((4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol. LCMS (C 23 H 26 F 2 N 8 O 2 ) (ES, m/z): 485 [M+H] + . 
       1 H NMR (400 MHz, Chloroform-d) δ 7.96 (d, J=10.7 Hz, 1H), 7.26 (s, 1H), 7.15 (t, J=3.8 Hz, 2H), 5.91 (s, 2H), 4.90 (dtt, J=48.1, 9.9, 4.7 Hz, 1H), 4.13 (s, 2H), 4.00 (s, 3H), 3.74-3.64 (m, 2H), 3.42 (d, J=12.6 Hz, 1H), 2.86 (t, J=11.4 Hz, 1H), 2.71 (dq, J=10.3, 7.2, 5.2 Hz, 2H), 2.17-1.92 (m, 3H), 1.88-1.73 (m, 2H), 1.57 (dq, J=18.2, 9.1 Hz, 2H). 
     The example compounds of the invention in the following Table 18 were prepared in a manner similar to that described for the preparation of Example 54 from the appropriate starting aryl halide and Intermediate 99. 
     
       
         
           
               
               
               
             
               
                 TABLE 18 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                   
                 Structure 
                 m/z 
               
               
                 Example 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 55 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 473 
               
               
                   
               
               
                   
                 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1- 
                   
               
               
                   
                 yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 56 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 485 
               
               
                   
               
               
                   
                 9-fluoro-2-((3R,5S or 3S,5R)-5-fluoro-1-(1-(tetrahydro-2H- 
                   
               
               
                   
                 pyran-4-yl)-1H-pyrazol-4-yl)piperidin-3-yl)-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
               
               
                   
               
            
           
         
       
     
     Example 57: 1-((4-((1R,5R or 1S,5S)-1-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-azabicyclo[3.1.0]hexan-3-yl)-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 
     Step 1: N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((1R,5R or 1S,5S)-3-(1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)-3-azabicyclo[3.1.0]hexan-1-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     To a reaction vial containing of solution of 2-((1R,5R or 1S,5S)-3-azabicyclo[3.1.0]hexan-1-yl)-N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 97) (60.0 mg, 0.129 mmol) in THF (1.5 mL) was added 4-bromo-1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazole (Intermediate 23) (61.1 mg, 0.194 mmol) followed by tBuXPhos-Pd G3 (30.8 mg, 0.039 mmol) and sodium tert-butoxide (43.4 mg, 0.452 mmol). Nitrogen was bubbled through the mixture for 10 min. The mixture was stirred and heated at 90° C. for 18 h. The mixture was cooled to room temperature. The solvents were evaporated, and the resulting residue was purified by preparative silica gel TLC with 5% MeOH in DCM as eluent to afford N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((1R,5R or 1S,5S)-3-(1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)-3-azabicyclo[3.1.0]hexan-1-yl)[1,2,4]triazolo[1,5-c]quinazolin-5-amine. LCMS (C 37 H 43 FN 8 O 5 ) (ES, m/z): 699 [M+H] + . 
     Step 2: 1-((4-((1R,5R or 1S,5S)-1-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-azabicyclo[3.1.0]hexan-3-yl)-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 2,2,2-trifluoroacetate 
     
       
         
         
             
             
         
       
     
     A mixture of N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((1R,5R or 1S,5S)-3-(1-((1-((tetrahydro-2H-pyran-2-yl)oxy)cyclobutyl)methyl)-1H-pyrazol-4-yl)-3-azabicyclo[3.1.0]hexan-1-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (59 mg, 0.084 mmol) and TFA (1.0 mL) was stirred and heated at 60° C. for 1 h. The mixture was cooled to room temperature. The solvents were evaporated. The resulting residue was purified by preparative silica gel TLC with 8% (7 M ammonia in MeOH) in DCM as eluent. The obtained residue was further purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm MeCN/H 2 O with 0.1% TFA modifier as eluent) to afford 1-((4-((1R,5R or 1S,5S)-1-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-azabicyclo[3.1.0]hexan-3-yl)-1H-pyrazol-1-yl)methyl)cyclobutan-1-ol 2,2,2-trifluoroacetate). LCMS (C 23 H 25 FN 8 O 2 ) (ES, m/z): 465 [M+H] + . 
       1 H NMR (400 MHz, Chloroform-d) δ 7.96 (d, J=10.0 Hz, 1H), 7.27 (s, 1H), 7.21 (s, 1H), 7.06 (s, 1H), 4.18 (s, 2H), 4.08 (s, 3H), 3.79 (d, J=8.6 Hz, 1H), 3.69 (d, J=8.7 Hz, 1H), 3.54 (d, J=8.6 Hz, 2H), 3.35 (s, 2H), 3.20 (dd, J=8.8, 3.8 Hz, 2H), 2.32 (s, 1H), 2.13-2.04 (m, 3H), 1.75 (d, J=3.8 Hz, 2H), 1.58 (d, J=4.9 Hz, 1H). 
     Example 58 in the following Table 19 was prepared in a manner similar to that described for the preparation of Example 57 from Intermediate 97 and the appropriate starting aryl halide. 
     
       
         
           
               
               
               
             
               
                 TABLE 19 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Exam- 
                 Structure 
                 m/z 
               
               
                 ple 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 58 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 453 
               
               
                   
               
               
                   
                 1-(4-((1R,5R or 1S,5S)-1-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3- 
                   
               
               
                   
                 azabicyclo[3.1.0]hexan-3-yl)-1H-pyrazol-1-yl)-2- 
                   
               
               
                   
                 methylpropan-2-ol 
               
               
                   
               
            
           
         
       
     
     Example 59: 9-fluoro-2-((3S,5R or 3R,5S)-5-fluoro-1-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)piperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     Step 1: N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3S,5R or 3R,5S)-5-fluoro-1-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)piperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     To a reaction vial was added N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3S,5R or 3R,5S)-5-fluoropiperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 100) (50.0 mg, 0.103 mmol), tBuXPhos-Pd G3 (24.6 mg, 0.0310 mmol), 4-bromo-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole (Intermediate 15) (23.9 mg, 0.103 mmol), sodium tert-butoxide (59.5 mg, 0.619 mmol) and THF (1 mL). The mixture was flushed with nitrogen for 5 min. The mixture was stirred and heated at 100° C. for 4 h. The solvents were evaporated, and the resulting residue was purified by silica gel chromatography with 0-100% (30% MeOH in EtOAc) in hexanes, yielding N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3S,5R or 3R,5S)-5-fluoro-1-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)piperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. 
     Step 2: 9-fluoro-2-((3S,5R or 3R,5S)-5-fluoro-1-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)piperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     To a reaction vial was added N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3S,5R or 3R,5S)-5-fluoro-1-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)piperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (85.0 mg, 0.130 mmol) was added TFA (2 mL). The mixture was stirred and heated at 60° C. for 1 h. The solvents were evaporated, and the residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm MeCN/H 2 O with 0.1% TFA modifier as eluent), to afford 9-fluoro-2-((3S,5R or 3R,5S)-5-fluoro-1-(1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazol-4-yl)piperidin-3-yl)-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. LCMS (C 23 H 26 F 2 N 8 O 2 ) (ES, m/z): 485 [M+H] + .  1 H NMR (500 MHz, Methanol-d 4 ) δ 7.93 (d, J=10.7 Hz, 1H), 7.51 (s, 1H), 7.38 (s, 1H), 7.23 (d, J=7.5 Hz, 1H), 4.95 (dt, J=10.3, 5.4 Hz, 1H), 4.32 (dq, J=11.0, 6.1, 5.6 Hz, 1H), 4.13-3.95 (m, 4H), 3.78 (d, J=11.1 Hz, 2H), 3.65-3.52 (m, 2H), 3.46 (t, J=11.2 Hz, 1H), 2.89 (t, J=11.4 Hz, 1H), 2.82-2.59 (m, 2H), 2.12-1.92 (m, 4H). 
     Example 60 and Example 61: (R or 5)-1-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)azepan-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and (S or R)-1-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)azepan-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     A 5 mL microwave vial was charged with rac-2-(azepan-3-yl)-N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 83) (100 mg, 0.208 mmol) and THF (1.3 mL). To the mixture was added 1-(4-bromo-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 4) (91.0 mg, 0.420 mmol), followed by tBuXPhos-Pd G3 (66.1 mg, 0.0830 mmol) and sodium tert-butoxide (80.0 mg, 0.832 mmol). Nitrogen was bubbled through the mixture for 10 min. The mixture was stirred and heated at 90° C. for 12 h. The mixture was cooled to room temperature, and then the solids were removed by filtration and washed with DCM. The solvents of the filtrate were evaporated. The resulting residue was dissolved in TFA (802 μL, 10.4 mmol) and heated at 50° C. for 3 h. The mixture was cooled to room temperature, and the solvents were evaporated. The resulting residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm MeCN/H 2 O with 0.1% TFA modifier as eluent) to yield the racemic product. The racemic mixture was resolved by chiral SFC separation (Chiral Technologies OJ-H 21×250 mm column with 25% (isopropanol w/0.1% NH 4 OH modifier) as co-solvent), to afford (R or S)-1-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)azepan-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 60, first eluting peak) and (S or R)-1-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)azepan-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 61, second eluting peak). 
     For Example 60: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z): 469 [M+H] + . 1H NMR (499 MHz, DMSO-d6) δ 7.88 (d, J=11.0 Hz, 1H), 7.72 (d, J=26.0 Hz, 2H), 7.18 (d, J=7.9 Hz, 1H), 7.12 (s, 1H), 7.07 (s, 1H), 4.63 (s, 1H), 3.97 (s, 3H), 3.87 (s, 2H), 3.75 (dd, J=14.3, 3.9 Hz, 1H), 3.53 (dd, J=14.3, 10.0 Hz, 1H), 3.45 (dq, J=9.7, 5.0, 4.6 Hz, 1H), 3.37 (dd, J=14.0, 6.1 Hz, 1H), 3.23 (ddd, J=13.3, 7.6, 5.1 Hz, 1H), 2.07-1.82 (m, 3H), 1.71 (s, 1H), 1.58-1.45 (m, 2H), 1.03 (d, J=3.5 Hz, 6H). 
     For Example 61: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z): 469 [M+H] + . 1H NMR (499 MHz, DMSO-d6) δ 7.88 (d, J=11.0 Hz, 1H), 7.70 (s, 2H), 7.18 (d, J=7.7 Hz, 1H), 7.13 (s, 1H), 7.07 (s, 1H), 4.62 (s, 1H), 3.97 (s, 3H), 3.87 (s, 2H), 3.75 (dd, J=14.4, 3.8 Hz, 1H), 3.53 (dd, J=14.2, 10.2 Hz, 1H), 3.45 (dt, J=9.5, 4.9 Hz, 1H), 3.38 (s, 1H), 3.24 (dd, J=13.6, 5.5 Hz, 2H), 2.08-1.84 (m, 3H), 1.71 (s, 1H), 1.50 (d, J=12.7 Hz, 2H), 1.03 (d, J=3.4 Hz, 6H). 
     The example compounds of the invention in the following Table 20 were prepared in a manner similar to that described for the preparation of Example 60 and Example 61 from the appropriate starting amine and aryl halide, where the resulting isomeric mixture of the corresponding final compounds were separated by SFC. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 20 
               
               
                   
               
               
                   
                   
                   
                 Observed 
               
               
                 Exam- 
                 Structure 
                 SFC 
                 m/z 
               
               
                 ple 
                 Name 
                 Conditions 
                 [M + H] +   
               
               
                   
               
             
            
               
                 62 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AD-H 21 × 250 mm column with 50% (IPA w/ 0.2% DIPA modifier) as co-solvent 
                 459 
               
               
                   
               
               
                   
                 (R or S)-1-(4-(3-(5-amino-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3- 
                   
                   
               
               
                   
                 fluoropyrrolidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 63 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AD-H 21 × 250 mm column with 50% (IPA w/ 0.2% DIPA modifier) as co-solvent 
                 459 
               
               
                   
               
               
                   
                 (S or R)-1-(4-(3-(5-amino-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3- 
                   
                   
               
               
                   
                 fluoropyrrolidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 64 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies IC 21 × 250 mm column with 35% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 470 
               
               
                   
               
               
                   
                 (R or S)-3-(3-((R)-3-(5-amino-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)- 
                   
                   
               
               
                   
                 1H-1,2,4-triazol-1-yl)-2-methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 65 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies IC 21 × 250 mm column with 35% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 470 
               
               
                   
               
               
                   
                 (S or R)-3-(3-((R)-3-(5-amino-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)- 
                   
                   
               
               
                   
                 1H-1,2,4-triazol-1-yl)-2-methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 66 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralcel OJ- H 4.6 × 150 mm column with 40% (MeOH w/ 0.05% DEA modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 1-(4-((3S or 3R, 4S or 4R)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 67 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralcel OJ- H 4.6 × 150 mm column with 40% (MeOH w/ 0.05% DEA modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 1-(4-((3R or 3S,4R or 4S)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
               
               
                   
               
            
           
         
       
     
     Example 68 and Example 69: 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     Step 1: rac-1-(4-((3R,5S or 3S,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 40 mL vial was added rac-N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3R,5S or 3S,5R)-5-fluoropiperidin-3-yl)-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 102) (736 mg, 1.52 mmol), 1-(4-bromo-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 4) (998 mg, 4.56 mmol), tBuXPhos-Pd G3 (965 mg, 1.22 mmol), sodium tert-butoxide (876 mg, 9.11 mmol), and THF (15.0 mL). The mixture was purged with nitrogen for 5 min. The mixture was stirred and heated at 80° C. for 6 h. The mixture was cooled to room temperature. The solvents were evaporated, and the resulting residue was purified by silica gel chromatography with 0-100% EtOAc:EtOH (3:1) in hexanes as eluent to afford rac-1-(4-((3R,5S or 3S,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol. LCMS (C 31 H 36 F 2 N 8 O 4 ) (ES, m/z): 623 [M+H] + . 
     Step 2: 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((3S,5R or 3R,55)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added rac-1-(4-((3R,5S or 3S,5R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (550 mg, 0.883 mmol), and TFA (8.83 mL, 115 mmol). The mixture was stirred and heated at 50° C. for 2 h. The solvents were evaporated. To the resulting residue was added MeOH and the mixture was filtered. The solvents of the filtrate were evaporated. The racemic mixture was resolved by chiral SFC separation (Chiral Technologies AS-H 21×250 mm column with 15% (MeOH w/0.1% NH 4 OH modifier) as co-solvent) to afford 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 68, first eluting peak) and 1-(4-((3S,5R or 3R,55)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 69, second eluting peak). 
     For Example 68: LCMS (C 22 H 26 F 2 N 8 O 2 ) (ES, m/z): 473 [M+H] + . 1H NMR (600 MHz, DMSO-d6) δ 7.82 (s, 2H), 7.43 (dd, J=8.3, 2.6 Hz, 1H), 7.37 (s, 1H), 7.28 (s, 1H), 7.18 (dd, J=11.0, 2.6 Hz, 1H), 4.94 (dtt, J=48.3, 10.3, 4.8 Hz, 1H), 4.65 (s, 1H), 3.93 (s, 3H), 3.89 (s, 2H), 3.78-3.71 (m, 1H), 3.66 (d, J=11.5 Hz, 1H), 3.40 (t, J=11.8 Hz, 1H), 2.75 (t, J=11.5 Hz, 1H), 2.66 (d, J=6.1 Hz, 1H), 2.58 (td, J=10.4, 5.2 Hz, 1H), 1.92 (p, J=11.3 Hz, 1H), 1.04 (s, 6H). 
     For Example 69: LCMS (C 22 H 26 F 2 N 8 O 2 ) (ES, m/z): 473 [M+H] + . 1H NMR (600 MHz, DMSO-d6) δ 7.82 (s, 1H), 7.44 (dd, J=8.3, 2.7 Hz, 1H), 7.37 (s, 1H), 7.28 (s, 1H), 7.19 (dd, J=11.1, 2.7 Hz, 1H), 4.95 (ddt, J=48.3, 10.4, 5.2 Hz, 1H), 4.64 (s, 1H), 3.94 (s, 2H), 3.89 (s, 1H), 3.74 (d, J=10.6 Hz, 1H), 3.66 (d, J=11.9 Hz, 1H), 3.40 (t, J=11.8 Hz, 1H), 2.74 (d, J=11.5 Hz, 1H), 2.65 (s, 1H), 2.58 (dt, J=10.3, 5.2 Hz, 1H), 1.97-1.89 (m, 1H), 1.04 (s, 6H). 
     The example compounds of the invention in the following Table 21 were prepared in a manner similar to that described for the preparation of Example 68 and Example 69 from Intermediate 102 and the appropriate starting aryl halide, where the resulting isomeric mixture of the corresponding final compounds were separated by SFC. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 21 
               
               
                   
               
               
                   
                   
                   
                 Observed  
               
               
                 Exam- 
                 Structure 
                   
                 m/z 
               
               
                 ple 
                 Name 
                 SFC Conditions 
                 [M + H] +   
               
               
                   
               
             
            
               
                 70 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Phenomenex Lux-2 21 × 250 mm column with 40% (MeOH w/ 0.1% NH 4 OH modifier) as  co-solvent 
                 487 
               
               
                   
               
               
                   
                 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 fluoropiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 71 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Phenomenex Lux-2 21 × 250 mm column with 40% (MeOH w/ 0.1% NH 4 OH modifier) as co- solvent 
                 487 
               
               
                   
               
               
                   
                 1-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 fluoropiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 72 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; ES Industries CCA 21 × 250 mm column with 20% (MeOH w/ 0.1% NH 4 OH modifier) as co- solvent  
                 485 
               
               
                   
               
               
                   
                 1-((4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 fluoropiperidin-1-yl)-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)methyl)cyclobutan-1-ol 
                   
                   
               
               
                   
               
               
                 73 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; ES Industries CCA 21 × 250 mm column with 20% (MeOH w/ 0.1% NH 4 OH modifier) as co- solvent 
                 485 
               
               
                   
               
               
                   
                 1-((4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 fluoropiperidin-1-yl)-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)methyl)cyclobutan-1-ol 
               
               
                   
               
            
           
         
       
     
     Example 74 and Example 75: 1-(4-((3R,5R or 3S,5S)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((3S,5S or 3R,5R)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     Step 1: rac-1-(4-((3R,5R or 3S,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added rac-N-(2,4-dimethoxybenzyl)-9-fluoro-2-((3R,5R or 3S,5S)-5-fluoropiperidin-3-yl)-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 102) (434 mg, 0.896 mmol), 1-(4-bromo-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 4) (589 mg, 2.69 mmol), tBuXPhos-Pd G3 (569 mg, 0.717 mmol), sodium tert-butoxide (517 mg, 5.37 mmol) and THF (9.0 mL). The mixture was purged with nitrogen for 5 min. The mixture was stirred and heated at 80° C. for 6 h. The mixture was cooled to room temperature. The solvents were evaporated. The resulting residue was purified by silica gel chromatography with 30-50% EtOAc:EtOH (3:1) in hexane as eluent, yielding rac-1-(4-((3R,5R or 3S,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol. LCMS (C 31 H 36 F 2 N 8 O 4 ) (ES, m/z): 623 [M+H] + . 
     Step 2: 1-(4-((3R,5R or 35,55)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((3S,5S or 3R,5R)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial containing rac-1-(4-((3R,5R or 3S,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (439 mg, 0.705 mmol) was added TFA (7.05 mL, 92.0 mmol). The mixture was stirred and heated at 50° C. for 2 h. The solvents were evaporated. To the residue was added MeOH. The mixture was filtered, and then the solvents of the filtrate were evaporated. The racemic mixture was resolved by chiral SFC separation (Chiral Technologies AS-H 21×250 mm column with 15% (MeOH w/0.1% NH 4 OH modifier) as co-solvent), yielding 1-(4-((3R,5R or 3S,5S)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 74, first eluting peak) and 1-(4-((3R,5R or 35,55)-3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 75, second eluting peak). 
     For Example 74: LCMS (C 22 H 26 F 2 N 8 O 2 ) (ES, m/z): 473 [M+H] + . 1H NMR (499 MHz, DMSO-d6) δ 7.83 (s, 2H), 7.43 (dd, J=8.4, 2.7 Hz, 1H), 7.32 (s, 1H), 7.24 (s, 1H), 7.19 (d, J=10.0 Hz, 1H), 5.11 (d, J=46.5 Hz, 1H), 4.64 (s, 1H), 3.94 (s, 3H), 3.89 (s, 2H), 3.69-3.48 (m, 3H), 2.97-2.89 (m, 1H), 2.90-2.79 (m, 1H), 2.40 (s, 1H), 2.14 (dt, J=40.9, 11.8 Hz, 1H), 1.04 (s, 6H). 
     For Example 75: LCMS (C 22 H 26 F 2 N 8 O 2 ) (ES, m/z): 473 [M+H] + . 1H NMR (499 MHz, DMSO-d6) δ 7.83 (s, 2H), 7.43 (dd, J=8.4, 2.7 Hz, 1H), 7.32 (s, 1H), 7.24 (s, 1H), 7.19 (d, J=8.9 Hz, 1H), 5.11 (d, J=47.5 Hz, 1H), 3.94 (s, 3H), 3.89 (s, 2H), 3.67-3.50 (m, 3H), 2.97-2.90 (m, 1H), 2.90-2.79 (m, 1H), 2.41 (s, 1H), 2.14 (dt, J=40.8, 12.8 Hz, 1H), 1.04 (s, 6H). 
     The example compounds of the invention in the following Table 22 were prepared in a manner similar to that described for the preparation of Example 74 and Example 75 from Intermediate 103 and the appropriate starting aryl halide, where the resulting isomeric mixture of the corresponding final compounds were separated by SFC. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 22 
               
               
                   
               
               
                   
                   
                   
                 Observed 
               
               
                 Exam- 
                 Structure 
                 SFC 
                 m/z 
               
               
                 ple 
                 Name 
                 Conditions 
                 [M + H] +   
               
               
                   
               
             
            
               
                 76 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Phenomenex Lux-3 21 × 250 mm column with 15% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 487 
               
               
                   
               
               
                   
                 1-(4-((3R,5R or 3S,5S)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 fluoropiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 77 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Phenomenex Lux-2 21 × 250 mm column with 15% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 487 
               
               
                   
               
               
                   
                 1-(4-((3S,5S or 3R,5R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 fluoropiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 78 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies OJ-H 21 × 250 mm column with 20% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 485 
               
               
                   
               
               
                   
                 1-((4-((3R,5R or 3S,5S)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 fluoropiperidin-1-yl)-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)methyl)cyclobutan-1-ol 
                   
                   
               
               
                   
               
               
                 79 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies OJ-H 21 × 250 mm column with 20% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 485 
               
               
                   
               
               
                   
                 1-((4-((3S,5S or 3R,5R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 fluoropiperidin-1-yl)-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)methyl)cyclobutan-1-ol 
               
               
                   
               
            
           
         
       
     
     Example 80 and Example 81: 1-(4-((3R,5R or 3S,5S)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((3S,5S or 3R,5R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     Step 1: rac-1-(4-((3R,5R or 3S,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     Step 1 of Example 80 and Example 81 was conducted in a manner similar to step 1 of Example 74 and Example 75, with Intermediate 101 and Intermediate 4 to afford rac-1-(4-((3R,5R or 3S,5S)-3-(5 #2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol. LCMS (C 31 H 36 F 2 N 8 O 4 ) (ES, m/z): 623 [M+H] + . 
     Step 2: 1-(4-((3R,5R or 35,55)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((3S,5S or 3R,5R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     Step 2 of Example 80 and Example 81 was conducted in a manner similar to step 2 of Example 74 and Example 75, where rac-1-(4-((3R,5R or 3S,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol is converted to the racemic mixture of the corresponding final compounds. The racemic mixture was resolved by chiral SFC separation (Chiral Technologies OJ-H 21×250 mm column with 25% (MeOH w/0.2% DIPA modifier) as co-solvent), to afford 1-(4-((3R,5R or 3S,5S)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 80, first eluting peak) and 1-(4-((3S,5S or 3R,5R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 81, second eluting peak). 
     For Example 80: LCMS (C 22 H 26 F 2 N 8 O 2 ) (ES, m/z): 473 [M+H] + .  1 H NMR (500 MHz, Methanol-d 4 ) δ 7.94 (d, J=10.8 Hz, 1H), 7.48 (s, 1H), 7.42 (s, 1H), 7.24 (d, J=7.5 Hz, 1H), 5.12 (d, J=46.8 Hz, 1H), 4.02 (d, J=3.8 Hz, 3H), 3.84 (d, J=12.4 Hz, 1H), 3.82-3.74 (m, 1H), 3.74-3.62 (m, 1H), 3.11 (t, J=11.0 Hz, 1H), 3.08-2.95 (m, 1H), 2.67 (s, 1H), 2.59 (s, 1H), 2.31-2.12 (m, 1H), 1.16 (s, 6H). 
     For Example 81: LCMS (C 22 H 26 F 2 N 8 O 2 ) (ES, m/z): 473 [M+H] + .  1 H NMR (500 MHz, Methanol-d 4 ) δ 7.94 (d, J=10.8 Hz, 1H), 7.50 (s, 1H), 7.43 (s, 1H), 7.24 (d, J=7.5 Hz, 1H), 5.13 (d, J=47.0 Hz, 1H), 4.02 (d, J=4.8 Hz, 3H), 3.85 (d, J=11.0 Hz, 1H), 3.82-3.73 (m, 1H), 3.73-3.60 (m, 1H), 3.11 (t, J=11.0 Hz, 1H), 3.04 (dd, J=36.0, 12.9 Hz, 1H), 2.59 (s, 1H), 2.30-2.12 (m, 1H), 1.16 (s, 6H). 
     Example 82 and Example 83: (1R or 1S,2R or 2S)-2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclopentan-1-ol and (1S or 1R,2S or 2R)-2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclopentan-1-ol 
     Step 1: (1R or 1S,2R or 2S)-2-(4-((R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclopentan-1-ol and (1S or 1R,2S or 2R)-2-(4-((R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclopentan-1-ol 
     
       
         
         
             
             
         
       
     
     To a reaction vial containing a solution of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 82) (150 mg, 0.322 mmol) in THF (3.0 mL) was added 2-(4-bromo-1H-pyrazol-1-yl)-1-methylcyclopentanol 2,2,2-trifluoroacetate (Intermediate 6) (184 mg, 0.514 mmol) followed by tBuXPhos-Pd G3 (77.0 mg, 0.0960 mmol) and sodium tert-butoxide (93.0 mg, 0.965 mmol). Nitrogen was bubbled through the mixture for 10 min. The mixture was stirred and heated at 90° C. for 4 h. The mixture was cooled to room temperature, and then the resulting residue was purified by silica gel chromatography with 0-40% EtOAc:EtOH (3:1) in hexane as eluent, yielding a diastereomeric mixture of products. The mixture was purified by SFC separation (Chiral Technologies OJ-H 21×250 mm column with 30% MeOH as co-solvent), yielding peak 1 and peak 2 corresponding to (1R or 1S,2R or 2S)-2-(4-((R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclopentan-1-ol and (1S or 1R,2S or 2R)-2-(4-((R)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclopentan-1-ol. For peak 1, LCMS (C 33 H 39 FN 8 O 4 ) (ES, m/z): 631 [M+H] + . For peak 2, LCMS (C 33 H 39 FN 8 O 4 ) (ES, m/z): 631 [M+H] + . 
     Step 2: (1R or 1S,2R or 2S)-2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclopentan-1-ol and (1S or 1R,2S or 2R)-2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclopentan-1-ol 
     
       
         
         
             
             
         
       
     
     Step 2 of Example 82 and Example 83 was conducted in a manner similar to step 2 of Example 40, where peak 1 and peak 2 obtained from step 1 were converted to (1R or 1S,2R or 2S)-2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclopentan-1-ol and (1S or 1R,2S or 2R)-2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclopentan-1-ol, which are the final compounds of Example 82 and Example 83, respectively. 
     For Example 82: LCMS (C 24 H 29 FN 8 O 2 ) (ES, m/z): 481 [M+H] + .  1 H NMR (400 MHz, Chloroform-d) δ 7.97 (d, J=10.7 Hz, 1H), 7.31 (s, 1H), 7.13 (d, J=7.6 Hz, 1H), 7.07 (s, 1H), 5.89 (s, 2H), 4.36 (t, J=8.8 Hz, 1H), 3.99 (s, 3H), 3.68 (dd, J=11.5, 3.6 Hz, 1H), 3.38 (tt, J=7.5, 3.8 Hz, 2H), 2.95 (t, J=11.1 Hz, 1H), 2.67 (td, J=11.3, 4.4 Hz, 1H), 2.40-2.07 (m, 3H), 1.99-1.73 (m, 7H), 0.88 (s, 3H). 
     For Example 83: LCMS (C 24 H 29 FN 8 O 2 ) (ES, m/z): 481 [M+H] + .  1 H NMR (400 MHz, Chloroform-d) δ 7.97 (d, J=10.7 Hz, 1H), 7.31 (s, 1H), 7.13 (d, J=7.6 Hz, 1H), 7.07 (s, 1H), 5.88 (s, 2H), 4.35 (t, J=8.7 Hz, 1H), 3.99 (s, 3H), 3.74-3.64 (m, 1H), 3.43-3.31 (m, 2H), 2.95 (t, J=11.1 Hz, 1H), 2.67 (td, J=11.2, 4.5 Hz, 1H), 2.39-2.10 (m, 3H), 2.00-1.74 (m, 7H), 0.88 (s, 3H). 
     The example compounds of the invention in the following Table 23 were prepared in a manner similar to that described for the preparation of Example 82 and Example 83 from the appropriate intermediates and starting materials. In each case, an SFC separation was conducted on the intermediate mixture formed from the first step. The SFC conditions to isolate these intermediates are shown with the corresponding final products formed from the second step. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 23 
               
               
                   
               
               
                   
                   
                 SFC Conditions 
                 Observed 
               
               
                 Exam- 
                 Structure 
                 for intermediate 
                 m/z 
               
               
                 ple 
                 Name 
                 from step 1 
                 [M + H] +   
               
               
                   
               
             
            
               
                 84 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Whelko- 1 21 × 250 mm column with 50% (MeOH w/ 0.2% DIPA modifier) as co-solvent 
                 467 
               
               
                   
                 (1R or 1S,2R or 2S)-2-(4-((R)-3-(5-amino-9- 
                   
                   
               
               
                   
                 fluoro-8-methoxy-[1,2,4[triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol- 
                   
                   
               
               
                   
                 1-yl)cyclopentan-1-ol 
                   
                   
               
               
                   
               
               
                 85 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Whelko- 1 21 × 250 mm column with 50% (MeOH w/ 0.2% DIPA modifier) as co-solvent 
                 467 
               
               
                   
               
               
                   
                 (1S or 1R,2S or 2R)-2-(4-((R)-3-(5-amino-9- 
                   
                   
               
               
                   
                 fluoro-8-methoxy-[1,2,4[triazolo]1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol- 
                   
                   
               
               
                   
                 1-yl)cyclopentan-1-ol 
                   
                   
               
               
                   
               
               
                 86 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AD-H 21 × 250 mm column with 45% (IPA 1:1 w/ 0.2% DIPA modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 (S or R)-3-(4-((R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4[triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-3-methyl-1H-pyrazol- 
                   
                   
               
               
                   
                 1-yl)-2-methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 87 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AD-H 21 × 250 mm column with 45% (IPA 1:1 w/ 0.2% DIPA modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 (R or S)-3-(4-((R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylbutan-2-ol 
               
               
                   
               
            
           
         
       
     
     Example 88: (1R or 1S,2R or 2S)-2-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)cyclopentan-1-ol 
     
       
         
         
             
             
         
       
     
     The mixture of N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S or 3S,6R)-6-methyl-1-(14(1R or 1S,2R or 2S)-2-((tetrahydro-2H-pyran-2-yl)oxy)cyclopentyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 120) (5.0 mg, 7.0 μmol) in TFA (0.3 mL) was heated at 60° C. for 25 min. The mixture was concentrated. The residue was purified by preparative silica gel TLC eluting with 5% (7 M ammonia in MeOH) in DCM to afford (1R or 1S,2R or 2S)-2-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)cyclopentan-1-ol. LCMS (C 24 H 29 FN 8 O 2 ) (ES, m/z): 481 [M+H] + .  1 H NMR (400 MHz, Chloroform-d) δ 7.99 (d, J=10.7 Hz, 1H), 7.25 (s, 1H), 7.15 (d, J=7.6 Hz, 1H), 7.02 (s, 1H), 5.87 (s, 2H), 4.35 (d, J=7.5 Hz, 1H), 4.21 (d, J=8.7 Hz, 1H), 4.01 (s, 3H), 3.74 (d, J=6.5 Hz, 1H), 3.47-3.41 (m, 1H), 3.32 (d, J=11.0 Hz, 1H), 3.23 (d, J=11.3 Hz, 1H), 2.26 (m, 1H), 2.16-2.08 (m, 3H), 2.08-2.00 (m, 2H), 1.90-1.71 (m, 4H), 1.13 (d, J=6.7 Hz, 3H). 
     Example 89: (1S or 1R,2S or 2R)-2-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)cyclopentan-1-ol 
     
       
         
         
             
             
         
       
     
     Example 89 was prepared from Intermediate 121 in a manner similar to Example 88 to afford (1S or 1R,2S or 2R)-2-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)cyclopentan-1-ol. LCMS (C 24 H 29 FN 8 O 2 ) (ES, m/z): 481 [M+H] + .  1 H NMR (400 MHz, Chloroform-d) δ 7.99 (d, J=10.7 Hz, 1H), 7.25 (s, 1H), 7.15 (d, J=7.6 Hz, 1H), 7.01 (s, 1H), 5.81 (s, 2H), 4.36 (q, J=7.1 Hz, 1H), 4.27-4.15 (m, 1H), 4.00 (s, 3H), 3.79-3.67 (m, 1H), 3.44 (dd, J=11.5, 4.2 Hz, 1H), 3.37-3.28 (m, 1H), 3.22 (t, J=11.1 Hz, 1H), 2.34-2.21 (m, 1H), 2.19-1.96 (m, 4H), 1.94-1.62 (m, 6H), 1.12 (d, J=6.7 Hz, 3H). 
     Example 90 and 91: 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((3S,5R or 3R,55)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a solution of rac-1-(4-((3S,5R or 3R,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 122) (150 mg, 0.242 mmol) in DCM (2 mL) was added TFA (2 mL). The mixture was stirred at 50° C. for 10 h. The mixture was concentrated. The racemic mixture was purified and resolved by chiral SFC (Phenomenex Lux-2 4.6×150 mm column with 0-40% (EtOH w/0.05% DEA) as cosolvent), to yield 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (first eluting peak) and 1-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (second eluting peak), corresponding to Example 90 and Example 91, respectively For Example 90: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z) [M+H] + : 469.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.66 (d, J=10.8 Hz, 1H), 7.32 (d, J=10.0 Hz, 2H), 6.94 (d, J=7.8 Hz, 1H), 3.98 (s, 2H), 3.91 (s, 3H), 3.81-3.71 (m, 1H), 3.38 (br d, J=9.0 Hz, 1H), 3.32-3.22 (m, 2H), 2.69 (t, J=11.6 Hz, 1H), 2.32-2.13 (m, 2H), 2.02-1.89 (m, 1H), 1.36 (q, J=12.5 Hz, 1H), 1.14 (s, 6H), 1.00 (d, J=6.6 Hz, 3H). 
     For Example 91: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z) [M+H] + : 469.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.68 (d, J=10.8 Hz, 1H), 7.37 (d, J=19.1 Hz, 2H), 6.98 (d, J=7.8 Hz, 1H), 3.99 (s, 2H), 3.93 (s, 3H), 3.84-3.76 (m, 1H), 3.42 (br d, J=10.3 Hz, 1H), 3.34-3.26 (m, 2H), 2.77 (t, J=11.6 Hz, 1H), 2.32-2.20 (m, 2H), 1.99 (br d, J=6.6 Hz, 1H), 1.40 (q, J=12.5 Hz, 1H), 1.19-1.09 (m, 7H), 1.02 (d, J=6.6 Hz, 3H). 
     Example 92: rac-1-(4-((3R,5R or 35,55)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a solution of 1-(4-((3R,5R and 3S,5S)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 123) (20 mg, 0.032 mmol) in DCM (2 mL) was added TFA (2 mL). The mixture was stirred and heated at 45° C. for 5 h. The mixture was concentrated. The residue was purified by reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm with MeCN/water (w/0.1% TFA as modifier) as eluent), to yield 1-(4-((3R,5R and 3S,5S)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol. LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z) [M+H] + : 469.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=8.02-7.90 (m, 2H), 7.73 (s, 1H), 7.27-7.15 (m, 1H), 4.22 (br d, J=11.2 Hz, 1H), 4.13-4.07 (m, 2H), 4.00 (s, 4H), 3.78-3.65 (m, 2H), 3.57-3.47 (m, 1H), 3.11 (t, J=11.0 Hz, 1H), 2.54 (br d, J=13.7 Hz, 1H), 2.27-2.14 (m, 1H), 1.90-1.80 (m, 1H), 1.17 (s, 6H), 1.11 (d, J=6.8 Hz, 3H). 
     Example 93: (R)-5-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)pentan-2-one 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of TFA (0.5 mL) in DCM (0.5 mL) was added (R)-5-(4-(3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)pentan-2-one (Intermediate 124) (28.0 mg, 0.0450 mmol). The mixture was stirred and heated at 50° C. for 15 h. The mixture was cooled, and the solvents were evaporated. The resulting residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm with MeCN/water (w/0.1% TFA as modifier) as eluent), to yield (R)-5-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)pentan-2-one. LCMS (C 23 H 27 FN 8 O 2 ) (ES, m/z) [M+H] + : 467. 
       1 H NMR (400 MHz, CD 3 OD) δ: 7.90 (d, J=10.96 Hz, 1H), 7.36 (s, 1H), 7.31 (s, 1H), 7.19 (d, J=7.45 Hz, 1H), 4.62 (br s, 1H), 4.05 (t, J=6.80 Hz, 2H), 3.99 (s, 3H), 3.67-3.78 (m, 1H), 3.39 (br d, J=11.84 Hz, 1H), 2.95 (t, J=11.18 Hz, 1H), 2.60-2.74 (m, 1H), 2.44 (t, J=7.02 Hz, 2H), 2.28 (br d, J=9.21 Hz, 1H), 2.10 (s, 3H), 2.02 (quin, J=7.02 Hz, 2H), 1.81-1.95 (m, 3H). 
     Example 94 and 95: (S or R)-5-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)pentan-2-ol and (R or S)-5-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)pentan-2-ol 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of (R)-5-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)pentan-2-one (Example 93) (15.0 mg, 0.0320 mmol) in THF (2 mL) was added NaBH 4  (2.4 mg, 0.064 mmol). The mixture was stirred at 25° C. for 3 h. The reaction mixture was quenched with 1 M aqueous HCl (2 mL). The mixture was extracted with EtOAc (2×30 mL), and the organic layer was dried over sodium sulfate, filtered and then the solvents of the filtrate were evaporated. The residue was purified by reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm with MeCN/water (w/0.1% TFA as modifier) as eluent), to afford (S or R)-5-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)pentan-2-ol and (R or S)-5-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)pentan-2-ol, corresponding to Example 94 and Example 95, respectively. 
     For Example 94: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z) [M+H] + : 469.  1 H NMR (400 MHz, CD 3 OD) δ: 7.94 (d, J=10.83 Hz, 1H), 7.89 (s, 1H), 7.67 (s, 1H), 7.25 (d, J=7.63 Hz, 1H), 4.19 (br t, J=7.10 Hz, 2H), 4.08-4.00 (m, 4H), 3.78-3.66 (m, 2H), 3.57 (br s, 2H), 3.36 (t, J=1.68 Hz, 1H), 2.40 (br s, 1H), 2.16-1.85 (m, 5H), 1.48-1.36 (m, 2H), 1.17 (d, J=6.26 Hz, 3H). 
     For Example 95: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z) [M+H] + : 469.  1 H NMR (400 MHz, CD 3 OD) δ: 7.95 (d, J=10.68 Hz, 1H), 7.46 (s, 1H), 7.37 (s, 1H), 7.24 (d, J=7.78 Hz, 1H), 4.11 (t, J=6.87 Hz, 3H), 4.03 (s, 4H), 3.84-3.62 (m, 3H), 3.48-3.37 (m, 5H), 3.31 (br s, 1H), 3.19 (s, 1H), 2.33 (s, 1H), 2.02-1.77 (m, 5H), 1.41 (br d, J=5.80 Hz, 2H), 1.16 (d, J=6.10 Hz, 3H). 
     Example 96 and Example 97: rac-(1r,4s or 1r,4r)-4-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclohexan-1-ol and rac-(1r,4r or 1r,4s)-4-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclohexan-1-ol 
     Step 1: 4-nitro-1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazole 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 1,4-dioxaspiro[4.5]decan-8-ol (5.00 g, 31.6 mmol), PPh 3  (12.4 g, 47.4 mmol), and 4-nitro-1H-pyrazole (4.29 g, 37.9 mmol) in DCM (80 mL) was added di-tert-butyl diazene-1,2-dicarboxylate (18.2 g, 79.0 mmol) under a nitrogen atmosphere, and the mixture was stirred at 25° C. for 10 h. The mixture was concentrated and purified by silica gel chromatography with 10-25% EtOAc in petroleum ether as eluent to afford 4-nitro-1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazole. LCMS (C 11 H 15 N 3 O 4 ) (ES, m/z) [M+H] + : 254. 
     Step 2: 1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-amine 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 4-nitro-1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazole (5.50 g, 21.7 mmol) in MeOH (50 mL) and EtOAc (50 mL) was added 10% Pd/C (0.462 g, 4.34 mmol). The mixture was purged with nitrogen twice and was stirred under an atmosphere of hydrogen for 6 h. The mixture was filtered and the solvents were evaporated. The resulting residue was purified by silica gel chromatography with 20-50% EtOAc in petroleum ether as eluent to afford 1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-amine. LCMS (C 11 H 17 N 3 O 2 ) (ES, m/z) [M+H] + : 224. 
     Step 3: methyl 1-(1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carboxylate 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-amine (2.14 g, 9.60 mmol) and lithium tetrafluoroborate (0.600 g, 6.40 mmol) in TFE (15 mL) was added ethyl 2-methylene-5-oxohexanoate (1.00 g, 5.88 mmol). The mixture was stirred and heated at 80° C. for 10 h. The mixture was cooled, diluted with water (15 mL), and extracted with EtOAc (2×30 mL). The organic layer was dried (anhydrous Na 2 SO 4 ), filtered, and the solvents of the filtrate were evaporated. To the resulting residue was added MeOH (15 mL) and 10% Pd/C (0.068 g, 0.64 mmol). The mixture was degassed and purged with nitrogen twice and was stirred under an atmosphere of hydrogen for 10 h. The mixture was filtered, and then the filtrate was concentrated. The residue was purified by silica gel chromatography with 10-50% EtOAc in petroleum ether as eluent to afford ethyl 1-(1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carboxylate. LCMS (C 20 H 31 N 3 O 4 ) (ES, m/z) [M+H] + : 378. 
     Step 4: 1-(1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carbohydrazide 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 1-(1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carboxylate (450 mg, 1.192 mmol) in EtOH (10 mL) was added hydrazine hydrate (382 mg, 11.92 mmol). The mixture was stirred and heated at 80° C. for 10 h. The mixture was concentrated to afford 1-(1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carbohydrazide, which was used without further purification. LCMS (C 18 H 29 N 5 O 3 ) (ES, m/z) [M+H] +  364. 
     Step 5: rac-2-((3R,6S or 3S,6R)-1-(1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-yl)-6-methylpiperidin-3-yl)-N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     A 40 mL vial was charged with 1-(1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carbohydrazide (351 mg, 0.967 mmol) and DCM (2 mL). To this mixture was added AcOH (0.025 mL, 0.44 mmol) followed by 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5-fluoro-4-methoxybenzonitrile (Intermediate 37) (300 mg, 0.879 mmol). The mixture was stirred and heated at 35° C. for 16 h. The mixture was then concentrated. The resulting residue was purified by silica gel chromatography with 10-50% EtOAc in petroleum ether as eluent to afford the cis diastereomer, 2-((3R,6S and 3S,6R)-1-(1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-yl)-6-methylpiperidin-3-yl)-N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. LCMS (C 36 H 43 FN 8 O 5 ) (ES, m/z) [M+H] +  687. 
     Step 6: 4-(4-((2S,5R and 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)cyclohexanone 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of 2-((3R,6S and 3S,6R)-1-(1-(1,4-dioxaspiro[4.5]decan-8-yl)-1H-pyrazol-4-yl)-6-methylpiperidin-3-yl)-N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (40.0 mg, 0.0580 mmol) in DCM (2 mL) was added TFA (2 mL). The mixture was stirred for 10 h. The mixture was concentrated. To the residue was added saturated aqueous sodium bicarbonate. The mixture was extracted with EtOAc (2×5 mL). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by preparative silica gel TLC with EtOAc as eluent to afford rac-4-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)cyclohexanone. LCMS (C 25 H 29 FN 8 O 2 ) (ES, m/z) [M+H] +  493. 
     Step 7: (1r,4s or 1r,4r)-4-(4-((2S,5R and 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclohexan-1-ol and (1r,4r or 1r,4s)-4-(4-((2S,5R and 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclohexan-1-ol 
     
       
         
         
             
             
         
       
     
     To a stirred mixture of rac-4-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)cyclohexanone (18 mg, 0.037 mmol) in THF (5 mL) was added methylmagnesium bromide (0.122 mL, 0.365 mmol). The mixture was stirred at 0° C. for 12 h. The reaction mixture was quenched with aqueous NH 4 Cl (5 mL) and extracted with EtOAc (2×5 mL). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm with MeCN/water (w/0.1% TFA modifier) as eluent) to afford (1r,4s or 1r,4r)-4-(4-((2S,5R and 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclohexan-1-ol and (1r,4r or 1r,4s)-4-(4-((2S,5R and 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclohexan-1-ol, corresponding to Example 96 and Example 97. 
     For Example 96:  1 H NMR (400 MHz, MeOD) δ 7.98 (s, 1H), 7.92 (d, J=11.2 Hz, 1H), 7.68 (s, 1H), 7.22 (d, J=7.6 Hz, 1H), 4.18-4.29 (m, 1H), 4.01 (s, 4H), 3.85-3.97 (m, 2H), 3.63-3.65 (m, 1H), 2.46-2.48 (m, 1H), 2.15-2.30 (m, 2H), 1.90-2.13 (m, 5H), 1.73-1.84 (m, 2H), 1.60-1.72 (m, 2H), 1.31 (s, 3H), 1.20 (d, J=6.8 Hz, 3H). LCMS (C 26 H 33 FN 8 O 2 ) (ES, m/z) [M+H] +  509. 
     For Example 97:  1 H NMR (400 MHz, MeOD) δ 8.01 (s, 1H), 7.91 (d, J=11.2 Hz, 1H), 7.70 (s, 1H), 7.21 (d, J=8.0 Hz, 1H), 4.06-4.25 (m, 2H), 4.00 (s, 3H), 3.84-3.98 (m, 2H), 3.65-3.68 (m, 1H), 2.48-2.50 (m, 1H), 2.10-2.32 (m, 3H), 2.02-2.08 (m, 2H), 1.87-1.96 (m, 2H), 1.80-1.82 (m, 2H), 1.56-1.67 (m, 2H), 1.25 (s, 3H), 1.21 (d, J=6.8 Hz, 3H). LCMS (C 26 H 33 FN 8 O 2 ) (ES, m/z) [M+H] +  509. 
     Example 98 and 99: (1S or 1R,2S or 2R)-2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclobutan-1-ol and (1R or 1S,2R or 2S)-2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclobutan-1-ol 
     
       
         
         
             
             
         
       
     
     A mixture of methylmagnesium bromide (0.318 ml, 0.955 mmol, 3 M in diethyl ether) in THF (1.00 ml) was cooled at 0° C. To the mixture was added 2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)cyclobutanone (Intermediate 125) (86.0 mg, 0.191 mmol) in THF (1.0 mL) The mixture was stirred at 0° C. for 5 h. To the mixture was added water (3 mL), and then the mixture was extracted with EtOAc (3×20 mL). The combined organic layers were dried over anhydrous Na 2 SO 4 , filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by preparative silica gel TLC with 10% MeOH in DCM as eluent. The isomeric mixture was purified by SFC (OJ-3 100×4.6 mm column with 5-40% (MeOH w/0.05% DEA) as co-solvent) to afford (1S or 1R,2S or 2R)-2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclobutan-1-ol (first eluting peak) and (1R or 1S,2R or 2S)-2-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-1-methylcyclobutan-1-ol (second eluting peak) corresponding to Example 98 and Example 99, respectively. 
     For Example 98: LCMS (C 23 H 27 FN 8 O 2 ) (ES, m/z) [M+H] + : 467.  1 H NMR (400 MHz, CD 3 OD) δ=7.98 (d, J=10.68 Hz, 1H), 7.37 (s, 1H), 7.15 (d, J=7.48 Hz, 1H), 7.09 (s, 1H), 5.78 (br s, 2H), 4.48-4.34 (m, 1H), 4.05-3.96 (m, 3H), 3.81-3.73 (m, 1H), 3.72-3.62 (m, 1H), 3.42-3.31 (m, 2H), 2.98 (t, J=11.06 Hz, 1H), 2.76-2.59 (m, 1H), 2.52-2.38 (m, 1H), 2.33-2.15 (m, 3H), 2.06-1.77 (m, 5H), 1.42 (s, 3H). 
     For Example 99: LCMS (C 23 H 27 FN 8 O 2 ) (ES, m/z) [M+H] + : 467.  1 H NMR (400 MHz, CD 3 OD) δ=7.98 (d, J=10.68 Hz, 1H), 7.37 (s, 1H), 7.15 (d, J=7.48 Hz, 1H), 7.09 (s, 1H), 5.78 (br s, 2H), 4.48-4.34 (m, 1H), 4.05-3.96 (m, 3H), 3.81-3.73 (m, 1H), 3.72-3.62 (m, 1H), 3.42-3.31 (m, 2H), 2.98 (t, J=11.06 Hz, 1H), 2.76-2.59 (m, 1H), 2.52-2.38 (m, 1H), 2.33-2.15 (m, 3H), 2.06-1.77 (m, 5H), 1.42 (s, 3H). 
     Example 100: (R)-9-fluoro-7-methoxy-2-(1-(1-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     BSA (2 mL, 8.18 mmol) was added to N′-(2-amino-6-fluoro-8-methoxyquinazolin-4-yl)-1-(1-methyl-1H-pyrazol-4-yl)piperidine-3-carbohydrazide (Intermediate 104) (25.0 mg, 0.0600 mmol), and the mixture was stirred at 120° C. for 2 h. The solvents were then evaporated. The resulting residue was diluted with chloroform/isopropanol—3:1 (5 mL), washed with aqueous sodium bicarbonate (saturated, 5 mL), and the organic layer collected using a phase separator column (25 mL) and concentrated. The residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm MeCN/water (with 0.1% TFA modifier) as eluent). The racemic mixture was resolved by chiral SFC separation (OJ-H column 21×250 mm column with 20% (MeOH w/0.25% DMEA modifier) as co-solvent) to afford (R or S)-9-fluoro-7-methoxy-2-(1-(1-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Example 100, first eluting peak). LCMS (C 19 H 21 FN 8 O) (ES, m/z): 397 [M+H] + .  1 H NMR (600 MHz, DMSO-d 6 ) δ 7.79 (s, 2H), 7.42 (dd, J=8.4, 2.7 Hz, 1H), 7.30 (s, 1H), 7.21-7.16 (m, 2H), 3.93 (s, 3H), 3.72 (s, 3H), 3.60 (dd, J=11.5, 3.7 Hz, 1H), 3.32-3.30 (m, 1H), 3.29-3.23 (m, 1H), 2.83 (t, J=11.1 Hz, 1H), 2.58-2.52 (m, 2H), 2.20-2.12 (m, 1H), 1.88-1.81 (m, 1H), 1.80-1.74 (m, 2H). 
     The example compounds of the invention in following Table 24 were prepared from the appropriate intermediates in a manner similar to Example 100, with the exception that the starting materials were enantiopure, thus no SFC separation was conducted for these examples. 
     
       
         
           
               
               
               
             
               
                 TABLE 24 
               
               
                   
               
               
                   
                   
                 Ob- 
               
               
                   
                   
                 served 
               
               
                 Ex- 
                   
                 m/z 
               
               
                 am- 
                 Structure 
                 [M + 
               
               
                 ple 
                 Name 
                 H] +   
               
               
                   
               
             
            
               
                 101 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 385 
               
               
                   
               
               
                   
                 (R or S)-8,9-difluoro-2-(1-(1-methyl-1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 102 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 367 
               
               
                   
               
               
                   
                 (R or S)-9-fluoro-2-(1-(1-methyl-1H-pyrazol-4-yl)piper- 
                   
               
               
                   
                 idin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 103 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 385 
               
               
                   
               
               
                   
                 (R or S)-7,9-difluoro-2-(1-(1-methyl-1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 104 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 413 
               
               
                   
               
               
                   
                 (R or S)-9-chloro-7-methoxy-2-(1-(1-methyl-1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 105 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 397 
               
               
                   
               
               
                   
                 (R or S)-9-chloro-7-methyl-2-(1-(1-methyl-1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 106 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 383 
               
               
                   
               
               
                   
                 (R or S)-9-chloro-2-(1-(1-methyl-1H-pyrazol-4-yl)piper- 
                   
               
               
                   
                 idin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 107 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 385 
               
               
                   
               
               
                   
                 (R or S)-9,10-difluoro-2-(1-(1-methyl-1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
               
               
                   
               
               
                 108 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 379 
               
               
                   
                 (R or S)-9-methoxy-2-(1-(1-methyl-1H-pyrazol-4- 
                   
               
               
                   
                 yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin- 
                   
               
               
                   
                 5-amine 
               
               
                   
               
            
           
         
       
     
     Example 109: 1-(4-((2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     Step 1: 1-(4-((2S,5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 100 mL round bottom flask was added (3R,6S)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carbohydrazide (Intermediate 61) (1.00 g, 3.39 mmol), 2-((((2,4-dimethoxybenzyl)imino)-methylene)amino)-5-fluoro-4-methoxybenzonitrile (Intermediate 37) (1.21 g, 3.55 mmol) and DCM (10 mL). The mixture was stirred at room temperature for 18 h. The mixture was concentrated, and the resulting residue was purified by silica gel chromatography with 0-100% EtOAc in hexane as eluent, yielding 1-(4-((2S,5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol. LCMS (C 32 H 39 FN 8 O 4 ) (ES, m/z): 619 [M+H] + . The absolute configuration of the product of Step 1 was assigned to be (2S,5R) using Vibrational Circular Dichroism (VCD) spectroscopy with confidence. Analysis was done comparing experimental data to the calculated VCD and IR spectra of the product possessing the (2S,5R) configuration. The experimental VCD spectrum of the product matched well with the calculated (2S,5R) spectrum over the region from 1000-1500 cm-1, resulting in an assignment of (2S,5R). 
     Step 2: 1-(4-((2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added 1-(4-((2S,5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (962 mg, 1.56 mmol) and TFA (7 mL). The mixture was stirred and heated at 50° C. for 1 h. The mixture was then concentrated. To the resulting residue was added 1 M aqueous HCl (50 mL) and DCM (50 mL). The aqueous layer was washed with DCM (2×50 mL), then filtered. The pH of the aqueous layer was then adjusted to ˜pH 10 with 10 M aqueous NaOH. The aqueous layer was extracted with 10% MeOH in DCM (2×100 mL). The organic layers were combined, dried over anhydrous sodium sulfate, filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-10% MeOH in DCM, yielding 1-(4-((2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 109). LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z): 469 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.90 (d, J=11.0 Hz, 1H), 7.72 (s, 2H), 7.20 (s, 1H), 7.19 (d, J=7.9 Hz, 1H), 7.15 (s, 1H), 3.69 (d, J=6.7 Hz, 1H), 3.35 (d, J=3.9 Hz, 1H), 3.20 (dt, J=11.1, 5.8 Hz, 1H), 3.10 (t, J=11.5 Hz, 1H), 2.00 (d, J=6.1 Hz, 3H), 1.70 (d, J=9.3 Hz, 1H), 1.03 (d, J=4.4 Hz, 9H). 
     The example compounds of the invention in the following Table 25 were prepared in a manner similar to that described for the preparation of Example 109 from the appropriate hydrazide and carbodiimide intermediates. 
     
       
         
           
               
               
               
             
               
                 TABLE 25 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Exam-  
                 Structure 
                 m/z 
               
               
                 ple 
                 Name 
                 [M + H] +   
               
               
                   
               
             
            
               
                 110 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 473 
               
               
                   
               
               
                   
                 1-(4-((2S,5R)-5-(5-amino-8-chloro-9-fluoro- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c[quinazolin-2-yl)-2-methylpiperidin-1- 
                   
               
               
                   
                 yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 111 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 457 
               
               
                   
               
               
                   
                 1-(4-((2S,5R)-5-(5-amino-7,9-difluoro-[1,2,4[triazolo[1,5- 
                   
               
               
                   
                 c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1- 
                   
               
               
                   
                 yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 112 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 453 
               
               
                   
               
               
                   
                 1-(4-((2S,5R)-5-(5-amino-9-fluoro-8-methyl- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1- 
                   
               
               
                   
                 yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 113 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 417 
               
               
                   
               
               
                   
                 (R)-7,9-dichloro-2-(1-(1-methyl-1H-pyrazol-4-yl)piperidin- 
                   
               
               
                   
                 3-yl)-[1,2,4[triazolo[1,5-c]quinazolin-5-amine 
               
               
                   
               
            
           
         
       
     
     Examples 114-117:1-(4-((2R or 2S,5R or 5S)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2S or 2R,5R or 5S)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2S or 2R,5S or 5R)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2R or 2S,5S or 5R)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     Step 1: 1-(4-(5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl) 2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 2 dram vial was added 1-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol-4-yl)-6-methylpiperidine-3-carbohydrazide (Intermediate 63) (133 mg, 0.431 mmol) and 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5-fluoro-3-methoxybenzonitrile (Intermediate 38) (140 mg, 0.410 mmol). To the mixture was added dioxane (1.6 mL) and acetic acid (24 μL, 0.41 mmol), and then the mixture was stirred and then heated at 60° C. for 2 h. The mixture was then allowed to slowly cool to room temperature. The mixture was diluted with DCM (10 mL) and washed with saturated aqueous sodium bicarbonate (2×10 mL) and brine. The combined organic layers were dried over anhydrous MgSO 4 , filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 10-100% EtOAc in hexanes to afford 1-(4-(5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol as a mixture of racemic diastereomers. LCMS (C 33 H 42 FN 8 O 4 ) (ES, m/z): 633 [M+H] +   
     Step 2: 1-(4-((2R or 2S,5R or 55)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2S or 2R,5R or 5S)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2S or 2R,5S or 5R)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((2R or 2S,5S or 5R)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added 1-(4-(5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (80 mg, 0.126 mmol) followed by TFA (0.13 mL). The mixture was stirred and heated at 50° C. for 2 h. The mixture was then concentrated. The resulting residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm with MeCN/water w/0.1% TFA as eluent) to afford the product as a mixture of diastereomers. The four isomers were resolved by chiral SFC separation (IC, 21×250 mm column with 35% (2-Propanol w/0.1% NH 4 OH modifier) as cosolvent) to afford 1-(4-((2R or 2S,5R or 5S)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 114, first eluting peak) and 1-(4-((2S or 2R,5R or 5S)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 115, second eluting peak) and 1-(4-((2S or 2R,5S or 5R)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 116, third eluting peak) and 1-(4-((2R or 2S,5S or 5R)-5-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 117, fourth eluting peak). 
     For Example 114: LCMS (C 24 H 32 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.70 (br s, 2H), 7.42 (dd, J=8.4, 2.7 Hz, 1H), 7.30 (s, 1H), 7.18 (dd, J=11.1, 2.7 Hz, 1H), 4.75 (br s, 1H), 3.93 (s, 3H), 3.80 (s, 2H), 3.37-3.31 (m, 1H), 3.23-3.16 (m, 1H), 2.18-2.15 (m, 1H), 2.01 (s, 3H), 2.09-1.94 (m, 2H), 1.70-1.59 (m, 1H), 1.25-1.10 (m, 2H), 1.04 (s, 3H), 1.03 (s, 3H), 0.95 (d, J=12.0 Hz, 3H). 
     For Example 115: LCMS (C 24 H 32 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.61 (br s, 2H), 7.40 (dd, J=8.2, 2.4 Hz, 1H), 7.30 (s, 1H), 7.18 (dd, J=11.0, 2.1 Hz, 1H), 4.75 (br s, 1H), 3.93 (s, 3H), 3.80 (s, 2H), 3.38-3.30 (m, 1H), 3.19-3.12 (m, 1H), 2.15-2.10 (m, 1H), 2.07 (s, 3H), 2.09-1.94 (m, 2H), 1.72-1.65 (m, 1H), 1.25-1.10 (m, 2H), 1.07 (s, 3H), 1.06 (s, 3H), 0.93 (d, J=11.8 Hz, 3H). 
     For Example 116: LCMS (C 24 H 32 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.52 (br s, 2H), 7.41 (dd, J=8.4, 2.7 Hz, 1H), 7.39 (s, 1H), 7.15-7.11 (m, 1H), 4.41 (br s, 1H), 3.90 (s, 3H), 3.85 (s, 2H), 3.30-3.20 (m, 1H), 3.13-3.09 (m, 1H), 2.18-2.11 (m, 1H), 2.00 (s, 3H), 2.00-1.84 (m, 2H), 1.75-1.66 (m, 1H), 1.24-1.10 (m, 2H), 1.04 (m, 6H), 0.91 (d, J=6.5 Hz, 3H). 
     For Example 117: LCMS (C 24 H 32 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.77 (br s, 2H), 7.43 (dd, J=8.4, 2.7 Hz, 1H), 7.33 (s, 1H), 7.18 (dd, J=11.1, 2.7 Hz, 1H), 4.60 (br s, 1H), 3.94 (s, 3H), 3.83 (s, 2H), 3.26-3.22 (m, 1H), 3.10-3.06 (m, 1H), 2.18-2.16 (m, 1H), 2.04 (s, 3H), 2.01-1.87 (m, 2H), 1.75-1.69 (m, 1H), 1.24-1.10 (m, 2H), 1.03 (s, 3H), 1.02 (s, 3H), 0.93 (d, J=6.5 Hz, 3H). 
     The example compounds of the invention in the following Table 26 were prepared in a manner similar to that described for the preparation of Examples 114-117 from the appropriate hydrazide and carbodiimide intermediates. The resulting isomeric mixtures were resolved by SFC separation. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 26 
               
               
                   
               
               
                   
                   
                   
                 Observed 
               
               
                 Exam- 
                 Structure 
                 SFC 
                 m/z 
               
               
                 ple 
                 Name 
                 Conditions 
                 [M + H] +   
               
               
                   
               
             
            
               
                 118 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Lux-4 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 1-(4-((2R or 2S,5R or 5S)-5-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 119 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Lux-4 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 1-(4-((2S or 2R,5S or 5R)-5-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 120 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Lux-4 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 1-(4-((2R or 2S,5S or 5R)-5-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 121 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 4; Lux-4 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 1-(4-((2S or 2R,5R or 5S)-5-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 122 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; IC-3 4.6 × 100 mm column with 40% (IPA w/ 0.05% DEA modifier) as cosolvent 
                 469 
               
               
                   
               
               
                   
                 2-(4-((2R or 2S,5R or 5S)-5-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 123 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; IC-3 4.6 × 100 mm column with 40% (IPA w/ 0.05% DEA modifier) as cosolvent 
                 469 
               
               
                   
               
               
                   
                 2-(4-((2S or 2R,5S or 5R)-5-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 124 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; IC-3 4.6 × 100 mm column with 40% (IPA w/ 0.05% DEA modifier) as cosolvent 
                 469 
               
               
                   
               
               
                   
                 2-(4-((2R or 2S,5S or 5R)-5-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 125 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 4; IC-3 4.6 × 100 mm column with 40% (IPA w/ 0.05% DEA modifier) as cosolvent 
                 469 
               
               
                   
               
               
                   
                 2-(4-((2S or 2R,5R or 5S)-5-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 126 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1 and 2 overlapping; Chiralcel OJ- 3 4.6 × 100 mm column with 5-40% (MeOH w/ 0.05% DEA modifier) as cosolvent 
                 469 
               
               
                   
               
               
                   
                 rac-2-(4-((2R or 2S,5R or 5S)-5-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 127 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Chiralcel OJ- 3 4.6 × 100 mm column with 5-40% (MeOH w/ 0.05% DEA modifier) as cosolvent 
                 469 
               
               
                   
               
               
                   
                 2-(4-((2R or 2S,5S or 5R)-5-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 128 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 4; Chiralcel OJ- 3 4.6 × 100 mm column with 5-40% (MeOH w/ 0.05% DEA modifier) as cosolvent 
                 469 
               
               
                   
               
               
                   
                 1-(4-((2S or 2R,5R or 5S)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
               
               
                   
               
            
           
         
       
     
     Example 129 and Example 130: (R)-9-fluoro-8-methoxy-2-(1-(3-methyl-1-((methylsulfonyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)-9-fluoro-8-methoxy-2-(1-(5-methyl-1-((methylsulfonyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     Step 1: mixture of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(3-methyl-1-((methylthio)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(5-methyl-1-((methylthio)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     A 1-dram vial was charged with the mixture of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(3-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(5-methyl-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 117) (391 mg, 0.715 mmol) and (chloromethyl)(methyl)sulfane (359 μL, 4.29 mmol) in dioxane (7.0 mL). To the mixture was added cesium carbonate (466 mg, 1.43 mmol), and then the mixture was stirred and heated at 75° C. for 60 h. The mixture was cooled to room temperature. The mixture was diluted with water and DCM. The mixture was poured into a phase separator. The DCM layer was collected and concentrated. The resulting residue was purified by silica gel column chromatography with 0-10% MeOH in DCM as eluent to afford the mixture of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(3-methyl-1-((methylthio)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(5-methyl-1-((methylthio)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine. LCMS (C 30 H 35 FN 8 O 3 S) (ES, m/z): 607 [M+H] + . 
     Step 2: (R)-9-fluoro-8-methoxy-2-(1-(3-methyl-1-((methylsulfonyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)-9-fluoro-8-methoxy-2-(1-(5-methyl-1-((methylsulfonyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine 
     
       
         
         
             
             
         
       
     
     The mixture of (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(3-methyl-1-((methylthio)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine and (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(1-(5-methyl-1-((methylthio)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (328 mg, 0.541 mmol) was dissolved in acetone (4 mL) and water (1.35 mL). To the mixture was added Oxone® (potassium peroxymonosulfate, 665 mg, 1.08 mmol). The mixture was stirred for 10 min at room temperature. The mixture was concentrated to remove the acetone, diluted with water and DCM, and the organic layer was collected via a phase separator. The organic layer was concentrated. To the resulting residue was added and TFA (2.08 mL, 27.0 mmol), and the mixture was stirred and heated at 50° C. for 16 h. The mixture was then diluted with water and DCM, and the DCM layer was collected via a phase separator and concentrated. The resulting residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm, MeCN/water (w/0.1% TFA modifier) as eluent) to afford (R)-9-fluoro-8-methoxy-2-(1-(3-methyl-1-((methylsulfonyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine, TFA (Example 129, first eluting peak) and (R)-9-fluoro-8-methoxy-2-(1-(5-methyl-1-((methylsulfonyl)methyl)-1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine, TFA, (Example 130, second eluting peak). 
     For Example 129: LCMS (C 21 H 25 FN 8 O 3 S) (ES, m/z): 489 [M+H] + .  1 H NMR (500 MHz, DMSO-d 6 ) δ 7.87 (d, J=10.9 Hz, 1H), 7.77 (s, 1H), 7.61 (s, 1H), 7.19 (d, J=7.9 Hz, 1H), 5.67 (s, 2H), 3.97 (s, 3H), 3.44 (m, 1H), 3.17 (s, 1H), 2.99 (s, 3H), 2.31 (s, 3H), 2.20 (m, 1H), 1.87 (m, 3H). 
     For Example 130: LCMS (C 21 H 25 FN 8 O 3 S) (ES, m/z): 489 [M+H] + .  1 H NMR (500 MHz, DMSO-d 6 ) δ 7.87 (dd, J=10.9, 3.6 Hz, 1H), 7.78 (s, 2H), 7.58 (s, 1H), 7.19 (d, J=7.8 Hz, 1H), 5.52 (s, 2H), 3.97 (s, 3H), 3.48 (d, J=9.4 Hz, 1H), 3.32 (d, J=10.1 Hz, 1H), 3.21 (d, J=11.2 Hz, 1H), 2.98 (s, 3H), 2.65 (m, 1H), 2.18 (s, 3H), 1.97-1.71 (m, 3H). 
     The example compounds of the invention in the following Table 27 were prepared in a manner similar to that described for the preparation of Example 129 and Example 130 from the appropriate intermediates and commercially available starting materials. 
     
       
         
           
               
               
               
             
               
                 TABLE 27 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Exam- 
                 Structure 
                 m/z [M + 
               
               
                 ple 
                 Name 
                 H] +   
               
               
                   
               
             
            
               
                 131 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 475 
               
               
                   
               
               
                   
                 (R)-9-fluoro-8-methoxy-2-(1-(1-((methylsulfonyl)methyl)-1H- 
                   
               
               
                   
                 pyrazo1-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c] 
                   
               
               
                   
                 quinazolin-5-amine 
                   
               
               
                   
               
               
                 132 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 525 
               
               
                   
               
               
                   
                 (R)-2-(1-(3-(difluoromethyl)-1-((methylsulfonyl)methyl)-1H- 
                   
               
               
                   
                 pyrazol-4-yl)piperidin-3-yl)-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
               
               
                   
               
            
           
         
       
     
     Example 133: (S or R)-1-(4-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     Step 1: (S or R)-1-(4-(3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a reaction vial was added (S or R)—N-(2,4-dimethoxybenzyl)-9-fluoro-2-(3-fluoropiperidin-3-yl)-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 90) (78 mg, 0.161 mmol), 1-(4-bromo-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 4) (52.9 mg, 0.241 mmol), tBuXPhos-Pd G3 (38.4 mg, 0.048 mmol), and sodium tert-butoxide (93 mg, 0.97 mmol) in THF (3 mL). The mixture was flushed with nitrogen for 10 min. The mixture was stirred and heated at 90° C. for 16 h. The mixture was cooled to room temperature, filtered, and the solvents of the filtrate were evaporated. The residue was purified by silica gel chromatography with 0-8% MeOH in DCM (with 0.2% NH 4 OH) as eluent to afford (S or R)-1-(4-(3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol. LCMS (C 31 H 36 F 2 N 8 O 4 ) (ES, m/z): 623 [M+H] + . 
     Step 2: (S or R)-1-(4-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     An 8 mL scintillation vial was charged with (S or R)-1-(4-(3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (70.0 mg, 0.112 mmol) and TFA (750 μL, 9.73 mmol). The mixture was stirred and heated at 40° C. for 2 h. The mixture was then cooled, and the solvents were evaporated. The resulting residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm MeCN/H 2 O with 0.05% TFA as eluent) to afford (S or R)-1-(4-(3-(5-amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-3-fluoropiperidin-1-yl)-1H-pyrazol-1-yl)-2 methylpropan-2-ol 2,2,2-trifluoroacetate. LCMS (C 22 H 26 F 2 N 8 O 2 ) (ES, m/z): 473 [M+H] + .  1 H NMR (500 MHz, DMSO-d 6 ) δ 7.89 (s, 2H), 7.48 (dd, J=8.3, 2.7 Hz, 1H), 7.30 (s, 1H), 7.24 (d, J=0.7 Hz, 1H), 7.21 (dd, J=11.1, 2.8 Hz, 1H), 3.93 (s, 3H), 3.88 (s, 2H), 3.68-3.47 (m, −1H), 3.45-3.42 (m, 1H), 3.22-3.08 (m, 1H), 2.84-2.65 (m, 1H), 2.45-2.18 (m, 2H), 1.99 (d, J=9.7 Hz, 1H), 1.80 (dd, J=9.0, 4.0 Hz, 1H), 1.03 (d, J=2.7 Hz, 6H). 
     Example 134 in the following Table 28 was prepared from Intermediate 91 and the appropriate starting materials in a manner similar to that described for the preparation of Example 133. 
     
       
         
           
               
               
               
             
               
                 TABLE 28 
               
               
                   
               
               
                 Ex- 
                   
                 Observed 
               
               
                 am- 
                 Structure 
                 m/z 
               
               
                 ple 
                 Name 
                 [M +H] +   
               
               
                   
               
             
            
               
                 134 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 473 
               
               
                   
               
               
                   
                 (R or S)-1-(4-(3-(5-amino-9-fluoro-7-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c[quinazolin-2-yl)-3-fluoropiper- 
                   
               
               
                   
                 idin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
               
               
                   
               
            
           
         
       
     
     Example 135: 1-(4-((2R or 2S,5S or 5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     A mixture of 1-(4-((2R or 2S,5S or 5R)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 113) (28.0 mg, 0.0440 mmol) in TFA (50.5 mg, 0.443 mmol) was heated at 60° C. for 1 h. The solvents were evaporated, and the resulting residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm MeCN/H 2 O with 0.1% TFA as eluent) to afford 1-(4-((2R or 2S,5S or 5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol.: LCMS (C 24 H 31 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (500 MHz, Methanol-d 4 ) δ 7.97-7.89 (m, 2H), 7.70 (s, 1H), 7.24 (d, J=7.5 Hz, 1H), 4.12 (s, 2H), 4.08-3.92 (m, 5H), 3.72-3.57 (m, 2H), 2.52-2.38 (m, 1H), 2.31-2.14 (m, 2H), 2.09-1.98 (m, 1H), 1.75-1.56 (m, 2H), 1.18 (s, 6H), 0.94 (t, J=7.4 Hz, 3H). 
     Example 136: 1-(4-((2S or 2R,5S or 5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     Example 136 was prepared from Intermediate 114 in a manner similar to that described for the preparation of Example 135. LCMS (C 24 H 31 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (500 MHz, Methanol-d 4 ) δ 7.93 (d, J=11.9 Hz, 2H), 7.70 (s, 1H), 7.24 (d, J=7.5 Hz, 1H), 4.12 (s, 2H), 4.07-3.93 (m, 5H), 3.69-3.58 (m, 2H), 2.51-2.37 (m, 1H), 2.32-2.15 (m, 2H), 2.10-1.99 (m, 1H), 1.74-1.58 (m, 2H), 1.17 (s, 6H), 0.94 (t, J=7.3 Hz, 3H). 
     Example 137: 1-(4-((2S or 2R,5S or 5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     Example 137 was prepared from Intermediate 115 in a manner similar to that described for the preparation of Example 135. LCMS (C 24 H 31 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (500 MHz, Methanol-d 4 ) δ 8.11 (s, 1H), 7.90-7.81 (m, 2H), 7.21 (d, J=7.5 Hz, 1H), 4.14 (d, J=20.7 Hz, 3H), 3.98 (d, J=12.7 Hz, 4H), 3.72-3.54 (m, 2H), 2.56 (d, J=16.8 Hz, 1H), 2.45 (d, J=20.5 Hz, 1H), 2.21-2.07 (m, 1H), 1.98-1.82 (m, 1H), 1.81-1.69 (m, 1H), 1.56-1.43 (m, 1H), 1.19 (s, 6H), 0.97 (t, J=7.3 Hz, 3H). 
     Example 138: 1-(4-((2S or 2R,5S or 5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-ethylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     Example 138 was prepared from Intermediate 116 in a manner similar to that described for the preparation of Example 135. LCMS (C 24 H 31 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (500 MHz, Methanol-d 4 ) δ 8.13 (s, 1H), 7.86 (d, J=13.4 Hz, 2H), 7.21 (d, J=7.6 Hz, 1H), 4.15 (d, J=13.4 Hz, 3H), 3.99 (d, J=8.8 Hz, 4H), 3.74-3.54 (m, 2H), 2.57 (d, J=11.0 Hz, 1H), 2.50-2.39 (m, 1H), 2.24-2.05 (m, 1H), 1.96-1.83 (m, 1H), 1.82-1.67 (m, 1H), 1.59-1.42 (m, 1H), 1.18 (s, 6H), 0.97 (t, J=7.5 Hz, 3H). 
     Example 139: rac-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)piperidin-2-one 
     Step 1: rac-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)piperidin-2-one 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added rac-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-2-one (Intermediate 89) (0.102 g, 0.212 mmol), 1-(4-iodo-1H-pyrazol-1-yl)-2-methylpropan-2-ol (0.172 g, 0.646 mmol), copper(I) iodide (42.7 mg, 0.224 mmol), potassium phosphate (267 mg, 1.26 mmol), and anhydrous DMF (2.1 mL). 
     The mixture was sparged with nitrogen for 5 min. To the mixture was added N 1 ,N 2 -dimethylethane-1,2-diamine (0.046 mL, 0.43 mmol). The mixture was stirred and heated at 100° C. for 2 h. The mixture was purified by reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm with MeCN/H 2 O (with 0.1% TFA) as eluent), to afford rac-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)piperidin-2-one. LCMS (C 31 H 35 FN 8 O 5 ) (ES, m/z): 619 [M+H] + . 
     Step 2: rac-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)piperidin-2-one 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added rac-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)piperidin-2-one (11.9 mg, 0.0192 mmol) and TFA (0.26 mL). The mixture was stirred and heated at 50° C. for 1 h. The mixture was concentrated. The residue was purified by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm with 
     MeCN/H 2 O (with 0.1% TFA) as eluent) to afford rac-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)piperidin-2-one. LCMS (C 22 H 25 FN 8 O 3 ) (ES, m/z): 469 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 8.05 (s, 1H), 7.88 (d, J=11.0 Hz, 1H), 7.79 (br s, 2H), 7.66 (s, 1H), 7.18 (d, J=7.8 Hz, 1H), 4.18 (t, J=7.8 Hz, 1H), 3.99-3.93 (m, 5H), 3.86-3.76 (m, 2H), 2.28-2.16 (m, 3H), 2.10-2.00 (m, 1H), 1.03 (s, 3H), 1.03 (s, 3H). 
     Example 140 and Example 141: (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     Step 1: Mixture of (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(morpholin-2-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 87) (49.4 mg, 0.105 mmol), a mixture of 1-(4-bromo-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-bromo-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 7) (31.0 mg, 0.133 mmol), tBuXPhos-Pd G3 (33.5 mg, 0.0422 mmol), sodium tert-butoxide (60.8 mg, 0.633 mmol), and anhydrous THF (1.5 mL). The mixture was sparged with nitrogen. The mixture was then stirred and heated at 100° C. for several minutes, then cooled to 23° C., and additional THF (1 mL) was added. The mixture was stirred and heated at 80° C. for 14 h. Additional amounts of the mixture of 1-(4-bromo-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-bromo-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 7) (42.5 mg, 0.182 mmol) and tBuXPhos-Pd G3 (33.5 mg, 0.0422 mmol) were added. The mixture was stirred and heated at 80° C. for 8 h. The mixture was diluted with DCM and MeOH and filtered through Celite® (diatomaceous earth). The filtrate was concentrated. The resulting residue was purified by silica gel chromatography with 0-100% EtOAc:EtOH (3:1) in hexanes as eluent to afford a mixture of (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol. LCMS (C 31 H 37 FN 8 O 5 ) (ES, m/z): 621 [M+H] + . 
     Step 2: (R)-1-(4-(2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and (R)-1-(4-(2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     To a 4 mL vial was added the mixture of (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (4.6 mg, 0.0074 mmol) and TFA (0.10 mL). The mixture was stirred at 23° C. for 2 h. The mixture was then stirred and heated at 50° C. for 50 min. To a separate vial was added the mixture of (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (35.6 mg, 0.0574 mmol) and TFA (0.78 mL), and this mixture was stirred and heated at 50° C. for 50 min. The contents of the two reaction vials were combined and concentrated to a residue. The residue was suspended in MeOH and filtered. The filtrate was concentrated to a residue. The resulting residue was subjected to chiral SFC separation (Chiral Technologies OJ-H 21×250 mm column with 15% (MeOH w/0.1% NH 4 OH modifier) as co-solvent), to afford (R)-1-(4-(2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 140) as peak 1, and a second peak. The second peak contained an impurity, and therefore was purified by SFC (Chiral Technologies AS-H 21×250 mm column with 20% (MeOH w/0.1% NH 4 OH modifier) as co-solvent), yielding (R)-1-(4-(2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)morpholino)-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 141). 
     For Example 140: LCMS (C 22 H 27 FN 8 O 3 ) (ES, m/z): 471 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.90 (d, J=10.9 Hz, 1H), 7.80 (br s, 2H), 7.37 (s, 1H), 7.18 (d, J=7.9 Hz, 1H), 4.95 (dd, J=10.0, 2.5 Hz, 1H), 4.62 (s, 1H), 4.07-4.00 (m, 1H), 3.97 (s, 3H), 3.88 (td, J=11.1, 2.3 Hz, 1H), 3.83 (s, 2H), 3.07-2.97 (m, 2H), 2.72 (td, J=11.5, 3.1 Hz, 1H), 2.12 (s, 3H), 1.03 (s, 3H), 1.02 (s, 3H). 
     For Example 141: LCMS (C 22 H 27 FN 8 O 3 ) (ES, m/z): 471 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.89 (d, J=10.9 Hz, 1H), 7.79 (br s, 2H), 7.34 (s, 1H), 7.18 (d, J=7.9 Hz, 1H), 4.95 (dd, J=9.7, 2.6 Hz, 1H), 4.63 (s, 1H), 4.08-4.00 (m, 1H), 3.96 (s, 3H), 3.92-3.82 (m, 3H), 3.25-3.20 (m, 1H), 3.11 (dd, J=11.5, 10.0 Hz, 1H), 2.96-2.90 (m, 1H), 2.87 (td, J=11.3, 3.1 Hz, 1H), 2.22 (s, 3H), 1.07 (s, 3H), 1.06 (s, 3H). 
     Examples 142-145: (R or S)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide and (S or R)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide and (R or S)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide and (S or R)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide 
     Step 1: mixture of 2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide and 2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added rac-2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)thiomorpholine 1,1-dioxide (Intermediate 88) (100 mg, 0.194 mmol), tBuXPhos-Pd G3 (154 mg, 0.194 mmol), a mixture of 1-(4-bromo-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-bromo-5-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 7) (140 mg, 0.598 mmol), and dry THF (3 mL). The mixture was sparged with nitrogen for 4 min. To the mixture was added sodium tert-butoxide (112 mg, 1.16 mmol). The mixture was stirred and heated at 80° C. for 18 h. The mixture was concentrated. The resulting residue was suspended in DCM, mixed with Celite® (diatomaceous earth), and filtered. The filtrate was concentrated. The resulting residue was purified by silica gel chromatography with 0-70% EtOAc:EtOH (3:1) in hexanes as eluent, yielding a mixture of rac-2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide and rac-2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide. LCMS (C 31 H 37 FN 8 O 6 S) (ES, m/z): 669 [M+H] + . 
     Step 2: (R or S)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide and (S or R)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide and (R or S)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide and (S or R)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added the mixture of rac-2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide and rac-2-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide (48.1 mg, 0.0719 mmol) and TFA (0.96 mL). The mixture was stirred and heated at 50° C. for 1 h. The mixture was concentrated to a residue. The resulting residue was suspended in MeOH and filtered. The filtrate was concentrated to a residue that was purified by SFC (Chiral Technologies AS-H 21×250 mm column with 20% (MeOH w/0.1% NH 4 OH modifier) as co-solvent) to afford four peaks that were concentrated. Each peak was subsequently purified individually by preparative reversed-phase HPLC (Waters SunFire C18 OBD Prep Column, 19 mm×100 mm with MeCN/H 2 O (with 0.1% TFA) as eluent) to afford the final compounds, (R or S)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide, and (S or R)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-3-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide, and (R or S)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide, and (S or R)-2-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-4-(1-(2-hydroxy-2-methylpropyl)-5-methyl-1H-pyrazol-4-yl)thiomorpholine 1,1-dioxide, corresponding to Example 142 (SFC peak 1), Example 143 (SFC peak 2), Example 144 (SFC peak 3), and Example 145 (SFC peak 4), respectively. 
     For Example 142: LCMS (C 22 H 27 FN 8 O 4 S) (ES, m/z): 519 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.91 (d, J=10.9 Hz, 1H), 7.88 (br s, 2H), 7.55 (s, 1H), 7.21 (d, J=7.8 Hz, 1H), 4.96 (dd, J=10.3, 3.5 Hz, 1H), 3.99 (s, 3H), 3.87-3.76 (m, 3H), 3.72-3.65 (m, 1H), 3.65-3.53 (m, 2H), 3.53-3.45 (m, 1H), 3.44-3.34 (m, 1H), 2.06 (s, 3H), 1.05 (s, 3H), 1.04 (s, 3H). 
     For Example 143: LCMS (C 22 H 27 FN 8 O 4 S) (ES, m/z): 519 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.91 (d, J=10.9 Hz, 1H), 7.88 (br s, 2H), 7.55 (s, 1H), 7.21 (d, J=7.8 Hz, 1H), 4.96 (dd, J=10.3, 3.5 Hz, 1H), 3.99 (s, 3H), 3.87-3.76 (m, 3H), 3.72-3.65 (m, 1H), 3.65-3.53 (m, 2H), 3.53-3.45 (m, 1H), 3.44-3.34 (m, 1H), 2.06 (s, 3H), 1.05 (s, 3H), 1.04 (s, 3H). 
     For Example 144: LCMS (C 22 H 27 FN 8 O 4 S) (ES, m/z): 519 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.89 (d, J=10.9 Hz, 1H), 7.86 (br s, 2H), 7.48 (s, 1H), 7.20 (d, J=7.8 Hz, 1H), 4.96 (dd, J=10.1, 3.4 Hz, 1H), 3.98 (s, 3H), 3.87-3.78 (m, 3H), 3.64-3.54 (m, 3H), 3.51-3.35 (m, 2H), 2.15 (s, 3H), 1.05 (s, 3H), 1.04 (s, 3H). 
     For Example 145: LCMS (C 22 H 27 FN 8 O 4 S) (ES, m/z): 519 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.89 (d, J=10.9 Hz, 1H), 7.86 (br s, 2H), 7.48 (s, 1H), 7.20 (d, J=7.8 Hz, 1H), 4.96 (dd, J=10.1, 3.4 Hz, 1H), 3.98 (s, 3H), 3.87-3.78 (m, 3H), 3.64-3.54 (m, 3H), 3.51-3.35 (m, 2H), 2.15 (s, 3H), 1.05 (s, 3H), 1.04 (s, 3H). 
     Example 146 and Example 147: (1R or 1S,3R or 3S)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol and (1S or 1R,3S or 3R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol 
     
       
         
         
             
             
         
       
     
     Intermediate 124 (70.0 mg, 0.170 mmol) was resolved by chiral SFC (AD 250×30 mm column with MeOH (0.1% NH 4 OH modifier) as cosolvent) to afford (1R or 1S,3R or 3S)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol (Example 146, first eluting peak) and (15 or 1R,3S or 3R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol (Example 147, second eluting peak). 
     For Example 146: LCMS (C 21 H 24 FN 7 O 2 ) (ES, m/z) [M+H] + : 426.  1 H NMR (400 MHz, MeOD-d 4 ) δ (ppm) 7.81 (s, 1H), 7.59-7.70 (m, 2H), 6.91 (br d, J=7.9 Hz, 1H), 4.20 (q, J=7.3 Hz, 2H), 3.88-3.99 (m, 3H), 2.89-3.01 (m, 1H), 2.75 (br d, J=13.2 Hz, 1H), 2.39 (br d, J=12.3 Hz, 1H), 1.97-2.13 (m, 2H), 1.88 (br dd, J=9.9, 3.3 Hz, 1H), 1.58-1.77 (m, 2H), 1.50-1.55 (m, 1H), 1.42-1.49 (m, 3H). 
     For Example 147: LCMS (C 21 H 24 FN 7 O 2 ) (ES, m/z) [M+H] + : 426.  1 H NMR (400 MHz, MeOD-d 4 ) δ (ppm) 7.71-7.87 (m, 2H), 7.64 (d, J=2.6 Hz, 1H), 6.96-7.18 (m, 1H), 4.20 (q, J=7.3 Hz, 2H), 3.97 (br d, J=13.2 Hz, 3H), 2.89-3.05 (m, 1H), 2.73 (br d, J=12.7 Hz, 1H), 2.40 (br d, J=12.7 Hz, 1H), 1.99-2.13 (m, 2H), 1.91 (br d, J=13.2 Hz, 1H), 1.62-1.79 (m, 2H), 1.52-1.59 (m, 1H), 1.47 (t, J=7.5 Hz, 3H). 
     Example 148 and Example 149: (15 or 1R,3R or 35)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol and (15 or 1R,3R or 35)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol 
     
       
         
         
             
             
         
       
     
     Intermediate 125 (50.0 mg, 0.120 mmol) was resolved by chiral SFC (AD 250×30 mm column with IPA (0.1% NH 4 OH modifier) as cosolvent) to afford (15 or 1R,3R or 35)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol (Example 148, first eluting peak) and (15 or 1R,3R or 35)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-ethyl-1H-pyrazol-4-yl)cyclohexan-1-ol (Example 149, second eluting peak). 
     For Example 148: LCMS (C 21 H 24  FN 7 O 2 ) (ES, m/z) [M+H] + : 426.  1 H NMR (400 MHz, MeOD-d 4 ) δ (ppm) 7.78-7.94 (m, 1H), 7.60 (s, 1H), 7.50 (s, 1H), 7.01-7.23 (m, 1H), 4.14 (q, J=7.2 Hz, 2H), 3.99 (br s, 3H), 3.46-3.56 (m, 1H), 2.38 (br d, J=14.0 Hz, 1H), 1.92-2.25 (m, 4H), 1.63-1.85 (m, 3H), 1.42 (t, J=7.2 Hz, 3H). 
     For Example 149: LCMS (C 21 H 24  FN 7 O 2 ) (ES, m/z) [M+H] + : 426.  1 H NMR (400 MHz, MeOD-d 4 ) δ (ppm) 7.81 (br s, 1H), 7.60 (s, 1H), 7.50 (s, 1H), 7.11 (br s, 1H), 4.09-4.20 (m, 2H), 3.96 (br s, 3H), 3.51 (br s, 1H), 2.38 (br d, J=13.6 Hz, 1H), 1.96-2.24 (m, 4H), 1.59-1.85 (m, 3H), 1.42 (t, J=7.2 Hz, 3H). 
     Example 150: (1R,3R or 1S,35)-3-(5-amino-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexan-1-ol 
     Step 1: ethyl 3-(((trifluoromethyl)sulfonyl)oxy)cyclohex-3-ene-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a 100 mL round bottom flask was added 2,6-di-tert-butylpyridine (11.1 ml, 49.4 mmol), ethyl 3-oxocyclohexane-1-carboxylate (6.32 ml, 35.3 mmol), and DCE (70.5 mL). The mixture was stirred and cooled at 0° C. To the mixture was added a 1 M solution in THF of Tf 2 O (45.8 mL, 45.8 mmol), dropwise over 5 min. The mixture was stirred for 30 min. The mixture was warmed to room temperature. After 2 h, the mixture was concentrated. To the resulting residue was added 1:1 DCM:hexanes (20 mL) and solids precipitated. The solids were removed by filtration. The filter cake was washed with 1:1 DCM:hexanes. The solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-100% EtOAc in hexanes as eluent, yielding ethyl 3-(((trifluoromethyl)sulfonyl)oxy)cyclohex-3-ene-1-carboxylate. 
     Step 2: ethyl 3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)cyclohex-3-ene-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a 100 mL round bottom flask was added potassium acetate (3.96 g, 40.4 mmol), Pd(dppf)Cl 2  (0.660 g, 0.808 mmol), bis(pinacolato)diboron (8.21 g, 32.3 mmol), and ethyl 3-(((trifluoromethyl)sulfonyl)oxy)cyclohex-3-ene-1-carboxylate (7.08 mL, 26.9 mmol). The flask was evacuated and refilled with nitrogen three times. To the flask was added DMA (40 mL). The mixture was stirred and heated at 90° C. for 16 h. The mixture was cooled to room temperature. The mixture was poured into a flask containing diethyl ether (150 mL). The mixture was stirred for 15 min. The solids were removed by filtration. The filtrate was washed with water (3×100 mL). The organic layer was dried over anhydrous magnesium sulfate, filtered, and the solvents were evaporated. The resulting residue was purified by silica gel chromatography with 0-30% EtOAc in hexanes as eluent to afford ethyl 3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)cyclohex-3-ene-1-carboxylate. LCMS (C 15 H 25 BO 4 ) (ES, m/z) [M+H] + : 281. 
     Step 3: ethyl (R or S)-3-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohex-3-ene-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a 100 mL flask was added Pd(dppf)Cl 2  (0.708 g, 0.968 mmol), K 3 PO 4  (15.4 g, 72.6 mmol), ethyl 3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)cyclohex-3-ene-1-carboxylate (7.12 g, 25.4 mmol), and 1-(4-bromo-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 4) (5.30 g, 24.2 mmol). To the flask was added dioxane (60 mL) and water (12 mL). The mixture was sparged with nitrogen for 5 min. The mixture was stirred and heated at 90° C. for 2 h. The mixture was diluted in EtOAc (10 mL) and filtered through Celite® (diatomaceous earth) topped with anhydrous sodium sulfate. The solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-70% EtOAc in hexanes as eluent, to afford the racemate. The racemate was resolved by chiral SFC (ES Industries CCA 21×250 mm column, 15% (MeOH w/NH 4 OH modifier) as cosolvent) to afford ethyl (R or S)-3-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohex-3-ene-1-carboxylate (first eluting peak). LCMS (C 16 H 24 N 2 O 3 ) (ES, m/z) [M+H] + : 293. 
     Step 4: ethyl (1R,3R or 1S,3S)-3-hydroxy-3-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexane-1-carboxylate 
     
       
         
         
             
             
         
       
     
     To a 250 mL round bottom flask was added (R or S)-3-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohex-3-ene-1-carboxylate (933 mg, 3.19 mmol), cobalt (II) acetylacetonate hydrate (220 mg, 0.798 mmol), and THF (50 mL). To the mixture was added phenylsilane (1.18 mL, 9.57 mmol), and the mixture was stirred, open to air, at room temperature for 5 days. To the mixture was added a 1 M solution of TBAF (6.38 mL, 6.38 mmol) in THF. The mixture was stirred for 15 min. The solvents were evaporated. The resulting residue was purified by silica gel chromatography with 0-10% MeOH in DCM as eluent, to afford the trans-diastereomer ethyl (1R,3R or 1S,3S)-3-hydroxy-3-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexane-1-carboxylate. LCMS (C 16 H 26 N 2 O 4 ) (ES, m/z) [M+H] + : 311. 
     Step 5: (1R,3R or 1S,3S)-3-hydroxy-3-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexane-1-carbohydrazide 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added ethyl (1R,3R or 1S,3S)-3-hydroxy-3-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexane-1-carboxylate (190 mg, 0.612 mmol), EtOH (1.5 mL), and hydrazine hydrate (0.210 ml, 3.67 mmol). The mixture was heated at 90° C. for 24 h. The solvents were evaporated to afford (1R,3R or 1S,3S)-3-hydroxy-3-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexane-1-carbohydrazide. LCMS (C 14 H 24 N 2 O 4 ) (ES, m/z) [M+H] + : 297. 
     Step 6: (1R,3R or 1S,3S)-3-(5-((2,4-dimethoxybenzyl)amino)-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexan-1-ol 
     
       
         
         
             
             
         
       
     
     The asterisks (*) in the above scheme indicate chiral centers. To a 20 mL vial was added (1R,3R or 1S,3S)-3-hydroxy-3-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexane-1-carbohydrazide (70.0 mg, 0.236 mmol), 2-((((2,4-dimethoxybenzyl)imino)methylene)amino)-5-fluoro-3-methoxybenzonitrile (105 mg, 0.307 mmol), dioxane (0.5 mL), and AcOH (7 μl, 0.12 mmol). The mixture was stirred and heated at 65° C. for 2 h. The solvents were evaporated. The residue was purified by silica gel chromatography with 0-100% EtOAc:EtOH (3:1) in hexanes as eluent to afford (1R,3R or 1S,35)-3-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylprop yl)-1H-pyrazol-4-yl)cyclohexan-1-ol. LCMS (C 32 H 39 N 7 O 5 ) (ES, m/z) [M+H] + : 602. 
     Step 7: (1R,3R or 1S,3S)-3-(5-amino-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexan-1-ol 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added DDQ (30.3 mg, 0.133 mmol) and DCM (1.0 mL). The mixture was cooled at 0° C. To the mixture was added water (0.05 mL). To the mixture was added (1R,3R or 1S,3S)-3-(5-((2,4-dimethoxybenzyl)amino)-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexan-1-ol (53.5 mg, 0.089 mmol) as a solution in DCM (1 mL). The mixture was stirred for 4 h. To the mixture was added 1 M aqueous KOH (20 mL), and then the mixture was extracted with DCM (2×20 mL). The organic layers were combined, dried over anhydrous sodium sulfate, filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography with 0-100% EtOAc:EtOH (3:1) in hexane as eluent. The product was further purified by chiral SFC (Chiral Technologies OJ-H 21×250 mm column, with 20% (MeOH with NH 4 OH modifier) as cosolvent) to afford (1R,3R or 1S,3S)-3-(5-amino-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-1-(1-(2-hydroxy-2-methylpropyl)-1H-pyrazol-4-yl)cyclohexan-1-ol (Example 150). LCMS (C 23 H 29 N 7 O 3 ) (ES, m/z) [M+H] + : 452.  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.73 (dd, J=8.0, 1.2 Hz, 3H), 7.56 (s, 1H), 7.39 (s, 1H), 7.29 (t, J=7.9 Hz, 1H), 7.24-7.10 (m, 1H), 4.81 (s, 1H), 4.65 (s, 1H), 3.95 (s, 2H), 3.90 (s, 3H), 3.48-3.40 (m, 1H), 2.22 (d, J=13.4 Hz, 1H), 2.09 (d, J=11.9 Hz, 1H), 1.98-1.84 (m, 3H), 1.73-1.58 (m, 3H), 1.16-0.89 (m, 9H). 
     Example 151: (R)-2-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol 
     
       
         
         
             
             
         
       
     
     To a 20 mL vial was added (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 82) (400 mg, 0.857 mmol), sodium tert-butoxide (330 mg, 3.43 mmol), 4-bromo-1-(2-methyl-1-((tetrahydro-2H-pyran-2-yl)oxy)propan-2-yl)-1H-pyrazole (Intermediate 151) (520 mg, 1.72 mmol), tBuXPhos-Pd G3 (272 mg, 0.343 mmol), and THF (5.7 mL). The mixture was purged with nitrogen for 5 min, sealed, and heated at 105° C. for 16 h. The reaction mixture was cooled to room temperature. To the mixture was added water (10 mL) and DCM (10 mL). The mixture was stirred for 10 min and filtered. The organic layer was collected with a phase separator. The solvents were evaporated. To the resulting residue was added TFA (3.8 mL, 49 mmol), and the mixture was heated at 50° C. for 3 h. The solvents were evaporated, and to the resulting residue was added DCM (10 mL), and a 7 M solution of ammonia in MeOH (1.07 mL, 7.52 mmol). The mixture was stirred for 1 h. The mixture was washed with water then brine. The organic layer was dried over anhydrous sodium sulfate, filtered, and the solvents of the filtrate were evaporated. The resulting residue was purified by silica gel chromatography column with 0-40% of MeOH in DCM as eluent to afford (R)-2-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol (Example 151). LCMS (C 22 H 27 FN 8 O 2 ) (ES, m/z): 455 [M+H] + .  1 H NMR (600 MHz, Methanol-d 4 ) δ 8.18 (s, 1H), 7.92 (d, J=10.7 Hz, 1H), 7.82 (s, 1H), 7.25 (d, J=7.6 Hz, 1H), 4.14 (dd, J=12.0, 3.5 Hz, 1H), 3.84 (dd, J=26.2, 11.7 Hz, 2H), 3.76 (s, 2H), 3.66 (dt, J=9.9, 5.9 Hz, 1H), 3.57-3.46 (m, 1H), 2.51-2.39 (m, 1H), 2.30-2.02 (m, 3H), 1.59 (s, 6H). 
     The example compounds of the invention in the following Table 29 were prepared from the appropriate starting aryl halide and amine intermediates in a manner similar to that described for the preparation of Example 151. 
     
       
         
           
               
               
               
             
               
                 TABLE 29 
               
               
                   
               
               
                   
                   
                 Observed 
               
               
                 Exam- 
                 Structure 
                 m/z [M + 
               
               
                 ple 
                 Name 
                 H] +   
               
               
                   
               
             
            
               
                 152 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 (R)-4-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4] 
                   
               
               
                   
                 triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H- 
                   
               
               
                   
                 pyrazol-1-yl)-2-methylbutan-2-ol 
                   
               
               
                   
               
               
                 153 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 491 
               
               
                   
               
               
                   
                 (R)-1-(4-(3-(5-amino-8-(difluoromethoxy)-9-fluoro- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)- 
                   
               
               
                   
                 1H-pyrazol-1-yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 154 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 505 
               
               
                   
               
               
                   
                 (R)-1-(4-(3-(5-amino-8-(difluoromethoxy)-9-fluoro- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-3- 
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
                   
               
               
                   
               
               
                 155 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 483 
               
               
                   
               
               
                   
                 racemic, trans-2-(4-(3-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin- 
                   
               
               
                   
                 1-yl)-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-1-ol 
                   
               
               
                   
               
               
                 156 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 469 
               
               
                   
               
               
                   
                 racemic, trans-2-(4-(3-(5-amino-9-fluoro-8-methoxy- 
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin- 
                   
               
               
                   
                 1-yl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol 
               
               
                   
               
            
           
         
       
     
     Example 157 and Example 158: 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol and 1-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol 
     
       
         
         
             
             
         
       
     
     A 20 mL microwave vial equipped with a stirbar was charged with rac, cis-N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(5-methylpiperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 83) (400 mg, 0.832 mmol) and THF (5.20 mL). To the mixture was added 1-(4-bromo-3-methyl-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Intermediate 24) (388 mg, 1.67 mmol), followed by tBuXPhos-Pd G3 (264 mg, 0.333 mmol) and sodium tert-butoxide (320 mg, 3.33 mmol). Nitrogen was bubbled through the mixture for 10 min. The vial was then sealed with a fresh cap and heated at 90° C. for 16 h. The reaction was cooled, quenched with saturated ammonium chloride (1 mL), and Celite was added. The biphasic mixture was filtered over Celite topped with anhydrous MgSO 4 , and the solvents of the filtrate were concentrated. The resulting residue was dissolved in TFA (3.2 mL, 42 mmol) and heated at 50° C. for 3 h. The reaction mixture was cooled, diluted with DCM, and quenched with saturated aqueous NaHCO 3 . The biphasic mixture was separated and the aqueous phase was further extracted with DCM. The organic layers were combined, dried over anhydrous MgSO 4 , filtered, and the solvents of the filtrate were concentrated. The resulting residue was purified by silica gel chromatography with 0-20% MeOH in DCM as eluent. The purified product was then subjected to chiral SFC separation (Chiral Technologies AD-H 21×250 mm column with 30% (IPA w/0.1% NH 4 OH modifier) as co-solvent), to afford 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 157, first eluting peak) and 1-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropan-2-ol (Example 158, second eluting peak). 
     For Example 157: LCMS (C 24 H 31 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.87 (d, J=11.0 Hz, 1H), 7.69 (s, 2H), 7.34 (s, 1H), 7.18 (d, J=7.9 Hz, 1H), 4.61 (s, 1H), 3.97 (s, 3H), 3.83 (s, 2H), 3.46 (d, J=10.9 Hz, 1H), 3.28 (d, J=11.8 Hz, 1H), 3.13 (d, J=8.6 Hz, 1H), 2.64 (t, J=11.3 Hz, 1H), 2.22 (d, J=13.4 Hz, 1H), 2.16 (t, J=11.1 Hz, 1H), 2.11 (s, 3H), 1.94 (s, 1H), 1.38 (q, J=12.3 Hz, 1H), 1.03 (d, J=3.1 Hz, 6H), 0.97 (d, J=6.6 Hz, 3H). For Example 158: LCMS (C 24 H 31 FN 8 O 2 ) (ES, m/z): 483 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.87 (d, J=11.0 Hz, 1H), 7.69 (s, 2H), 7.34 (s, 1H), 7.18 (d, J=7.8 Hz, 1H), 4.61 (s, 1H), 3.97 (s, 3H), 3.83 (s, 2H), 3.47 (d, J=8.4 Hz, 1H), 3.29 (s, 1H), 3.13 (d, J=8.0 Hz, 1H), 2.64 (t, J=11.3 Hz, 1H), 2.22 (d, J=12.5 Hz, 1H), 2.16 (t, J=11.0 Hz, 1H), 2.11 (s, 3H), 1.96 (s, 1H), 1.38 (q, J=12.4 Hz, 1H), 1.03 (d, J=3.1 Hz, 6H), 0.97 (d, J=6.6 Hz, 3H). 
     The example compounds of the invention in the following Table 30 were prepared from the appropriate starting amine and aryl halide in a manner similar to that described for the preparation of Example 157 and Example 158, where the resulting isomeric mixture of the corresponding final compounds were separated by SFC. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 30 
               
               
                   
               
               
                   
                   
                   
                 Observed 
               
               
                   
                   
                   
                 m/z 
               
               
                 Exam- 
                 Structure 
                   
                 [M + 
               
               
                 ple 
                 Name 
                 SFC Conditions 
                 H] +   
               
               
                   
               
             
            
               
                 159 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Cellulose-2 30 × 250 mm column with 5-15% (MeOH w/0.05% DEA modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 1-(4-(3R,5S or 3S,5R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 160 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Cellulose-2 30 × 250 mm column with 5-15% (MeOH w/0.05% DEA modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 1-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 161 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Cellulose-3 4.6 × 150 mm column with 5-15% (MeOH w/0.05% DEA modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 162 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Cellulose-3 4.6 × 150 mm column with 5-15% (MeOH w/0.05% DEA modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 1-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 163 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AS-H 21 × 250 mm column with 25% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 457 
               
               
                   
               
               
                   
                 1-(4-((3R,5S or 3S,5R)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 164 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AS-H 21 × 250 mm column with 25% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 457 
               
               
                   
               
               
                   
                 1-(4-((3S,5R or 3R,5S)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 165 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Lux-4 21 × 250 mm column with 40% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 2-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 166 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Lux-4 21 × 250 mm column with 40% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 2-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)- 
                   
                   
               
               
                   
                 5-methylpiperidin-1-yl)-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 167 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Lux-4 21 × 250 mm column with 30% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 471 
               
               
                   
               
               
                   
                 1-(4-((3R,5S or 3S,5R)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 168 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Lux-4 21 × 250 mm column with 30% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 471 
               
               
                   
               
               
                   
                 1-(4-((3S,5R or 3R,5S)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 169 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AD- 3 4.6 × 150 mm column with 0-40% (IPA w/0.05% DEA modifier) as co- solvent 
                 483 
               
               
                   
               
               
                   
                 2-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 170 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak AD- 3 4.6 × 150 mm column with 0-40% (IPA w/0.05% DEA modifier) as co- solvent  
                 483 
               
               
                   
               
               
                   
                 2-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 171 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (EtOH w/0.05% DEA modifier) as co- solvent 
                 469 
               
               
                   
               
               
                   
                 2-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 172 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (EtOH w/0.05% DEA modifier) as co- solvent 
                 469 
               
               
                   
               
               
                   
                 2-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 173 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; ES Industries CCA 21 × 250 mm column with 25% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 483 
               
               
                   
                 1-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-5-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 174 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; ES Industries CCA 21 × 250 mm column with 25% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 1-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-5-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 175 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (MeOH w/0.05% DEA modifier) as  co-solvent 
                 483 
               
               
                   
               
               
                   
                 2-(4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 176 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (MeOH w/0.05% DEA modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 2-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-7- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 177 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co-solvent 
                 471 
               
               
                   
               
               
                   
                 2-(4-((3R,5S or 3S,5R)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 178 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co-solvent 
                 471 
               
               
                   
               
               
                   
                 2-(4-((3S,5R or 3R,5S)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-3-methyl-1H-pyrazol-1-yl)- 
                   
                   
               
               
                   
                 2-methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 179 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies OJ-H 21 × 250 mm colum with 20% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 457 
               
               
                   
               
               
                   
                 2-(4-((3S,5S or 3R,5R)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 180 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies OJ-H 21 × 250 mm column with 20% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 457 
               
               
                   
               
               
                   
                 2-(4-((3R,5R or 3S,5S)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-1-ol 
                   
                   
               
               
                   
               
               
                 181 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies OJ-H 21 × 250 mm  column with 20% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 457 
               
               
                   
               
               
                   
                 1-(4-((3S,5S or 3R,5R)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 182 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies OJ-H 21 × 250 mm column with 20% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 457 
               
               
                   
               
               
                   
                 1-(4-((3R,5R or 3S,5S)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 183 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 (R or S)-1-(4-((R)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 184 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 (S or R)-1-(4-((R)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 185 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; ES Industries CCA 21 × 250 mm column with 30% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 455 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 186 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; ES Industries CCA 21 × 250 mm column with 30% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 455 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 187 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AS-H 21 × 250 mm column with 30% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 455 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 188 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AS-H 21 × 250 mm column with 30% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 455 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 189 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; ES Industries CCA 21 × 250 mm column with 15% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-3-methyl-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 190 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; ES Industries CCA 21 × 250 mm column with 15% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-3-methyl-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 191 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AD-H 21 × 250 mm column with 30% (MeOH w/ 0.1% NH 4 OH modifier) as co- solvent 
                 457 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((R)-3-(5-amino-7,9- 
                   
                   
               
               
                   
                 difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-3-methyl-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 192 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AD-H 21 × 250 mm column with 30% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 457 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((R)-3-(5-amino-7,9- 
                   
                   
               
               
                   
                 difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-3-methyl-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 193 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AD-H 21 × 250 mm column with 25% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-5-methyl-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 194 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AD-H 21 × 250 mm column with 25% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-5-methyl-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 195 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies IG 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-5-methyl-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 196 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies IG 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 469 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-5-methyl-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 197 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AS- 3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co-solvent 
                 443 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((R)-3-(5-amino-7,9- 
                   
                   
               
               
                   
                 difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 198 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak AS- 3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co- solvent 
                 443 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((R)-3-(5-amino-7,9- 
                   
                   
               
               
                   
                 difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 199 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies OJ-H 21 × 250 mm column with 15% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 455 
               
               
                   
               
               
                   
                 (2R,3S or 2S,3R)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 200 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies OJ-H 21 × 250 mm column with 15% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 455 
               
               
                   
               
               
                   
                 (2S,3R or 2R,3S)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 201 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AD-H 21 × 250 mm column with 35% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 455 
               
               
                   
               
               
                   
                 (2R,3S or 2S,3R)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 202 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AD-H 21 × 250 mm column with 35% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 455 
               
               
                   
               
               
                   
                 (2S,3R or 2R,3S)-3-(4-((R)-3-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 203 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AS- 3 4.6 × 100 mm column with 0-40% (IPA w/0.05% DEA modifier) as co-solvent 
                 443 
               
               
                   
               
               
                   
                 (2R,3S or 2S,3R)-3-(4-((R)-3-(5-amino-7,9- 
                   
                   
               
               
                   
                 difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 204 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak AS- 3 4.6 × 100 mm column with 0-40% (IPA w/0.05% DEA modifier) as co-solvent 
                 443 
               
               
                   
               
               
                   
                 (2S,3R or 2R,3S)-3-(4-((R)-3-(5-amino-7,9- 
                   
                   
               
               
                   
                 difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)piperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 205 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AD- 3 4.6 × 100 mm column with 5-40% (IPA w/0.05% DEA modifier) as co- solvent. Then Peak 1; Chiral Technologies AD-H 21 × 250 mm column with 20% (IPA w/0.1% NH 4 OH modifier) as co-solvent 
                 497 
               
               
                   
               
               
                   
                 (R or S)-3-(4-((3R,5S or 3S,5R)-3-(5-amino-9- 
                   
                   
               
               
                   
                 fluoro-7-methoxy-[1,2,4[triazolo[1,5-c]quinazolin- 
                   
                   
               
               
                   
                 2-yl)-5-methylpiperidin-1-yl)-3-methyl-1H- 
                   
                   
               
               
                   
                 pyrazol-1-yl)-3-methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 206 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AD- 3 4.6 × 100 mm column with 5-40% (IPA w/0.05% DEA modifier) as co- solvent. Then Peak 2; Chiral Technologies AD-H 21 × 250 mm column with 20% (IPA w/0.1% NH 4 OH modifier) as co-solvent 
                 497 
               
               
                   
               
               
                   
                 (S or R)-3-(4-((3R,5S or 3S,5R)-3-(5-amino-9- 
                   
                   
               
               
                   
                 fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin- 
                   
                   
               
               
                   
                 2-yl)-5-methylpiperidin-1-yl)-3-methyl-1H- 
                   
                   
               
               
                   
                 pyrazol-1-yl)-3-methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 207 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak AD- 3 4.6 × 100 mm column with 5-40% (IPA w/0.05% DEA modifier) as co-solvent. 
                 497 
               
               
                   
               
               
                   
                 (R or S)-3-(4-((3S,5R or 3R,5S)-3-(5-amino-9- 
                   
                   
               
               
                   
                 fluoro-7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin- 
                   
                   
               
               
                   
                 2-yl)-5-methylpiperidin-1-yl)-3-methyl-1H- 
                   
                   
               
               
                   
                 pyrazol-1-yl)-3-methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 208 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; ES Industries CC4 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. Then Peak 1; ES Industries CCA 21 × 250 mm column with 15% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((3R,5S or 3S,5R)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-8-methoxy-[1,2,4[triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 209 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; ES Industries CC4 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. Then Peak 2; ES Industries CCA 21 × 250 mm column with 15% (MeOH w/ 0.1% NH 4 OH modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((3R,5S or 3S,5R)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-8-methoxy-[1,2,4[triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 210 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; ES Industries CC4 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. 
                 483 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((3S,5R or 3R,5S)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 211 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; ES Industries CC4 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. 
                 483 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((3S,5R or 3R,5S)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 212 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AD-H 30 × 250 mm column with 5-40% (IPA w/ 0.05% DEA modifier) as co-solvent. 
                 471 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((3R,5S or 3S,5R)-3-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 213 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AD-H 30 × 250 mm column with 5-40% (IPA w/ 0.05% DEA modifier) as co-solvent. 
                 471  
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((3R,5S or 3S,5R)-3-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 214 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Chiral Technologies AD-H 30 × 250 mm column with 5-40% (IPA w/ 0.05% DEA modifier) as co-solvent. 
                 471 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((3S,5R or 3R,5S)-3-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 215 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 4; Chiral Technologies AD-H 30 × 250 mm column with 5-40% (IPA w/ 0.05% DEA modifier) as co-solvent. 
                 471 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((3S,5R or 3R,5S)-3-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 216 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; IG 50 × 250 mm column with 5- 40% (EtOH w/0.05% DEA modifier) as co- solvent. 
                 471 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((3R,5S or 3S,5R)-3-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-5- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 217 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; IG 50 × 250 mm column with 5- 40% (EtOH w/0.05% DEA modifier) as co- solvent. 
                 471 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((3R,5S or 3S,5R)-3-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-5- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 218 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; IG 50 × 250 mm column with 5- 40% (EtOH w/0.05% DEA modifier) as co-solvent. 
                 471 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((3S,5R or 3R,5S)-3-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-5- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 219 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 4; IG 50 × 250 mm column with 5- 40% (EtOH w/0.05% DEA modifier) as co- solvent. 
                 471 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((3S,5R or 3R,5S)-3-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-5- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 220 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co-solvent. 
                 483 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((3R,5S or 3S,5R)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-5- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 221 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co-solvent.  
                 483 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((3R,5S or 3S,5R)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-5- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 222 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((3S,5R or 3R,5S)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-5- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 223 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 4; Chiralpak AD- 3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co-solvent. 
                 483 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((3S,5R or 3R,5S)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-5- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 224 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AD-H 30 × 250 mm column with 5-40% (EtOH w/ 0.05% DEA modifier) as co-solvent. Then Peak 1; Chiral Technologies AD-H 30 × 250 mm column with 5-40% (IPA w/ 0.05% DEA modifier) as co-solvent. 
                 483 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((3R,5S or 3S,5R)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-7-methoxy-[1,2,4[triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 225 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies AD-H 30 × 250 mm column with 5-40% (EtOH w/ 0.05% DEA modifier) as co-solvent. Then Peak 2; Chiral Technologies AD-H 30 × 250 mm column with 5-40% (IPA w/ 0.05% DEA modifier) as co-solvent. 
                 483 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((3R,5S or 3S,5R)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-7-methoxy-[1,2,4[triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 226 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AD-H 30 × 250 mm column with 5-40% (EtOH w/ 0.05% DEA modifier) as co-solvent. 
                 483 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((3S,5R or 3R,5S)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3- 
                   
                   
               
               
                   
                 methyl-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 227 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Chiral Technologies AD-H 30 × 250 mm column with 5-40% (EtOH w/ 0.05% DEA modifier) as co-solvent. 
                 483 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((3S,5R or 3R,5S)-3-(5- 
                   
                   
               
               
                   
                 amino-9-fluoro-7-methoxy-[1,2,4]triazolo[1,5- 
                   
                   
               
               
                   
                 c]quinazolin-2-yl)-5-methylpiperidin-1-yl)-3-methyl- 
                   
                   
               
               
                   
                 1H-pyrazol-1-yl)butan-2-ol 
               
               
                   
               
            
           
         
       
     
     Example 228-231: (2S,3S or 2R,3R)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol and (2S,3S or 2R,3R)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol and (2R,3R or 2S,3S)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol and (2R,3R or 2S,3S)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
     
       
         
         
             
             
         
       
     
     Step 1: Mixture of diastereomers of (2S,3S and 2R,3R)-3-(4-((2S,5R or 2R,5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
     To solution of N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-((3R,6S and 3S,6R)-6-methyl-1-(1H-pyrazol-4-yl)piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 177) (530 mg, 0.970 mmol) in DMF (10 mL) was added cis-2,3-dimethyloxirane (846 μl, 9.70 mmol) and cesium carbonate (1.26 g, 3.88 mmol). The mixture was stirred and heated at 125° C. for 5 h. The mixture was cooled to room temperature, and the mixture was diluted with water (20 mL) and ethyl acetate (20 mL). The biphasic mixture was separated and the aqueous phase was further extracted with ethyl acetate (20 mL). The combined organic layers were then washed with water (2×20 mL) and brine (1×20 mL). The organic layer was dried over anhydrous MgSO 4 , filtered, and the solvents were evaporated. The residue was purified by silica gel chromatography with 0-20% MeOH in DCM as eluent to afford the intermediate mixture of diastereomers of (2S,3S and 2R,3R)-3-(4-((2S,5R or 2R,5S)-5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol. 
     Step 2: (2S,3S or 2R,3R)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol and (2S,3S or 2R,3R)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol and (2R,3R or 2S,3S)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol and (2R,3R or 2S,3S)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
     To a 20 mL vial was added 3-(4-(5-(5-((2,4-dimethoxybenzyl)amino)-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol (475 mg, 0.768 mmol) and TFA (1.77 mL 23.0 mmol). The mixture was stirred and heated at 50° C. for 3 h. The mixture was cooled at room temperature, and the solvents were evaporated. The residue was dissolved in MeOH (10 mL) and quenched with a 7 M solution of ammonia in MeOH (1.10 mL, 7.68 mmol). The mixture was stirred for 20 minutes. The mixture was filtered, rinsing the solids with MeOH, and the filtrate was concentrated. The residue was suspended in DCM and filtered to remove remaining ammonium salts. The filtrate was was loaded directly onto a silica gel column, eluting with 0-15% MeOH in DCM to afford a mixture of isomers. The mixture was submitted for chiral SFC separation (Phenomenex Lux-2 21×250 mm column with 45% (MeOH w/0.1% NH 4 OH modifier) as co-solvent), to afford a mixture of Example 228 and Example 229 (peak 1), (2R,3R or 2S,3S)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol (Example 230, peak 2) and (2R,3R or 2S,3S)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol (Example 231, peak 3). The mixture obtained in peak 1 was further purified by SFC separation (Chiral Technologies AS-H 21×250 mm column with 20% (MeOH w/0.1% NH 4 OH modifier) as co-solvent), to afford (2S,3S or 2R,3R)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol (Example 228, peak 1) and (2S,3S or 2R,3R)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol (Example 229, peak 2). 
     For Example 228: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z): 469 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.90 (d, J=10.6 Hz, 1H), 7.73 (s, 2H), 7.22 (s, 1H), 7.19 (d, J=7.4 Hz, 1H), 7.12 (s, 1H), 4.73 (d, J=5.0 Hz, 1H), 4.13-4.05 (m, 1H), 3.98 (s, 3H), 3.88-3.78 (m, 1H), 3.70 (s, 1H), 3.19 (s, 1H), 3.10 (t, J=11.5 Hz, 1H), 2.01 (d, J=21.4 Hz, 3H), 1.70 (d, J=9.1 Hz, 1H), 1.34 (d, J=6.9 Hz, 3H), 1.03 (d, J=6.6 Hz, 3H), 0.92 (d, J=6.1 Hz, 3H). 
     For Example 229: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z): 469 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.90 (d, J=8.2 Hz, 1H), 7.73 (s, 2H), 7.25-7.21 (m, 1H), 7.19 (d, J=4.8 Hz, 1H), 7.12 (d, J=2.9 Hz, 1H), 4.72 (s, 1H), 4.09 (s, 1H), 3.98 (d, J=2.7 Hz, 3H), 3.83 (s, 1H), 3.70 (s, 1H), 3.19 (s, 1H), 3.10 (t, J=10.5 Hz, 1H), 1.99 (s, 3H), 1.72 (s, 1H), 1.43-1.30 (m, 3H), 1.03 (d, J=3.4 Hz, 3H), 0.92 (d, J=3.0 Hz, 3H). 
     For Example 230: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z): 469 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.90 (d, J=11.0 Hz, 1H), 7.72 (s, 2H), 7.22 (s, 1H), 7.18 (d, J=7.8 Hz, 1H), 7.11 (s, 1H), 4.77-4.64 (m, 1H), 4.14-4.04 (m, 1H), 4.02-3.94 (m, 3H), 3.88-3.78 (m, 1H), 3.71 (d, J=9.9 Hz, 1H), 3.18 (dd, J=6.4, 4.4 Hz, 1H), 3.09 (t, J=11.4 Hz, 1H), 2.01 (d, J=22.2 Hz, 3H), 1.70 (d, J=10.4 Hz, 1H), 1.38-1.31 (m, 3H), 1.03 (d, J=6.4 Hz, 3H), 0.95-0.89 (m, 3H). 
     For Example 231: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z): 469 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.90 (d, J=9.6 Hz, 1H), 7.73 (s, 2H), 7.22 (s, 1H), 7.19 (d, J=6.7 Hz, 1H), 7.12 (s, 1H), 4.73 (d, J=3.3 Hz, 1H), 4.15-4.03 (m, 1H), 3.98 (s, 3H), 3.83 (d, J=5.2 Hz, 1H), 3.70 (s, 1H), 3.19 (s, 1H), 3.10 (t, J=11.0 Hz, 1H), 2.01 (d, J=21.8 Hz, 3H), 1.70 (d, J=9.6 Hz, 1H), 1.33 (d, J=5.7 Hz, 3H), 1.03 (d, J=5.4 Hz, 3H), 0.92 (d, J=5.0 Hz, 3H). 
     The example compounds of the invention in the following Table 31 were prepared in a manner similar to that described for the preparation of Example 228-231 from the appropriate starting materials and intermediates, where the resulting isomeric mixture of the corresponding final compounds were separated by SFC. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 31 
               
               
                   
               
               
                   
                   
                   
                 Observed 
               
               
                   
                 Structure 
                   
                 m/z  
               
               
                 Example 
                 Name 
                 SFC Conditions 
                 [M + H] +   
               
               
                   
               
             
            
               
                 232 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1 (mixture of Example 232 and Example 233); Phenomenex Lux-2  21 × 250 mm column with 45% (MeOH  w/0.1% NH 4 OH modifier) as co- solvent. Then Peak 1; Chiral Technologies AS-H 21 × 250 mm column with 20% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. 
                 457 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((2S,5R or 2R,5S)-5-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin- 
                   
                   
               
               
                   
                 2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 233 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1 (mixture of Example 232 and Example 233); Phenomenex Lux-2  21 × 250 mm column with 45% (MeOH  w/0.1% NH 4 OH modifier) as co- solvent. Then Peak 2; Chiral Technologies AS-H 21 × 250 mm column with 20% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. 
                 457 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((2S,5R or 2R,5S)-5-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin- 
                   
                   
               
               
                   
                 2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 234 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Phenomenex  Lux-2 21 × 250 mm  column with 45%  (MeOH w/0.1%  NH 4 OH modifier) as  co-solvent. 
                 457 
               
               
                   
               
               
                   
                 (2R,3R or 2S,3S)-3-(4-((2R,5S or 2S,5R)-5-(5- 
                   
                   
               
               
                   
                 amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin- 
                   
                   
               
               
                   
                 2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 235 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Phenomenex  Lux-2 21 × 250 mm  column with 45%  (MeOH w/0.1%  NH 4 OH modifier) as  co-solvent. 
                 457 
               
               
                   
               
               
                   
                 (2S,3S or 2R,3R)-3-(4-((2R,5S or 2S,5R)-5-(5-amino- 
                   
                   
               
               
                   
                 7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 236 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral  Technologies AD-H  21 × 250 mm column  with 40% (MeOH  w/0.1% NH 4 OH  modifier) as co- solvent. 
                 469 
               
               
                   
               
               
                   
                 (2S,3R or 2R,3S)-3-(4-((2S,5R or 2R,5S)-5-(5-amino- 
                   
                   
               
               
                   
                 9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 237 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral  Technologies AD-H  21 × 250 mm column  with 40% (MeOH  w/0.1% NH 4 OH  modifier) as co- solvent. 
                 469 
               
               
                   
               
               
                   
                 (2R,3S or 2S,3R)-3-(4-((2S,5R or 2R,5S)-5-(5-amino- 
                   
                   
               
               
                   
                 9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2- 
                   
                   
               
               
                   
                 yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 238 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Phenomenex  Lux-3 21 × 250 mm  column with 15%  (MeOH w/0.1%  NH 4 OH modifier) as  co-solvent. 
                 469 
               
               
                   
               
               
                   
                 (2S,3R or 2R,3S)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9- 
                   
                   
               
               
                   
                 fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)- 
                   
                   
               
               
                   
                 2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 239 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Phenomenex  Lux-3 21 × 250 mm  column with 15%  (MeOH w/0.1%  NH 4 OH modifier) as  co-solvent.  
                 469 
               
               
                   
               
               
                   
                 (2R,3S or 2S,3R)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9- 
                   
                   
               
               
                   
                 fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)- 
                   
                   
               
               
                   
                 2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)butan-2-ol 
                   
                   
               
               
                   
               
               
                 240 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral  Technologies AS-H  21 × 250 mm column  with 25% (MeOH  w/0.1% NH 4 OH  modifier) as co- solvent. 
                 469 
               
               
                   
               
               
                   
                 (R or S)-1-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)propan-2-ol 
                   
                   
               
               
                   
               
               
                 241 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral  Technologies AS-H  21 × 250 mm column  with 25% (MeOH  w/0.1% NH 4 OH  modifier) as co- solvent. 
                 469 
               
               
                   
               
               
                   
                 (S or R)-1-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)propan-2-ol 
               
               
                   
               
            
           
         
       
     
     Example 242 and Example 243: (R or S)-3-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-3-methylbutan-2-ol and (S or R)-3-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-3-methylbutan-2-ol 
     
       
         
         
             
             
         
       
     
     Step 1: (R)-3-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-3-methylbutan-2-one 
     A 5 mL microwave vial equipped with a stirbar was charged with (R)—N-(2,4-dimethoxybenzyl)-9-fluoro-8-methoxy-2-(piperidin-3-yl)-[1,2,4]triazolo[1,5-c]quinazolin-5-amine (Intermediate 82) (100 mg, 0.214 mmol) and THF (1.3 mL). To the mixture was added 3-(4-bromo-1H-pyrazol-1-yl)-3-methylbutan-2-one (Intermediate 137) (99.0 mg, 0.429 mmol), followed by tBuXPhos-Pd G3 (85.0 mg, 0.107 mmol) and sodium tert-butoxide (82.0 mg, 0.857 mmol). Nitrogen was bubbled through the mixture for 10 min. The vial was then sealed with a fresh cap and heated at 105° C. for 16 h. The reaction mixture was cooled to room temperature, and to the mixture was added water (10 mL) and DCM (10 mL). The mixture was stirred for 10 min and filtered. The organic layer was collected and concentrated. To the resulting residue was added TFA (826 μL, 10.7 mmol), and the mixture was heated at 50° C. for 3 h. The solvents were evaporated. The resulting residue was dissolved in MeOH (5 mL), and to the mixture was added a 7 M solution of ammonia in MeOH (1.53 mL, 10.7 mmol). The mixture was stirred for 30 min and filtered. The solids were washed with methanol. The filtrate was concentrated. The residue was dissolved in DCM, and the resulting solution was washed with water. The organic layer was dried over anhydrous sodium sulfate, filtered, and the solvents were evaporated. The resulting residue was purified by silica gel chromatography with 5-30% MeOH in DCM as eluent to afford (R)-3-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-3-methylbutan-2-one LCMS (C 23 H 27 FN 8 O 2 ) (ES, m/z): 467 [M+H] + . Step 2: (R or S)-3-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-3-methylbutan-2-ol and (S or R)-3-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-3-methylbutan-2-ol 
     To a solution of (R)-3-(4-(3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-3-methylbutan-2-one (49.0 mg, 0.105 mmol) in EtOH (1 mL) was added NaBH 4  (11.9 mg, 0.315 mmol), and the mixture was stirred at room temperature for 1 h. The solvents were evaporated. To the resulting residue was added DCM, and the mixture was washed with water. The organic layer was dried over anhydrous sodium sulfate, filtered, and the solvents of the filtrate were evaporated to afford a mixture of isomers. The mixture was submitted for SFC chiral separation (Chiral Technologies IA 21×250 mm column with 45% (MeOH w/0.1% NH 4 OH modifier) as co-solvent), to afford (R or S)-3-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-3-methylbutan-2-ol (Example 242, peak 1) and (S or R)-3-(4-((R)-3-(5-amino-9-fluoro-8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)-1H-pyrazol-1-yl)-3-methylbutan-2-ol (Example 243, peak 2). 
     For Example 242: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z): 469 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.88 (dd, J=10.9, 2.3 Hz, 1H), 7.71 (s, 2H), 7.42-7.34 (m, 1H), 7.24-7.07 (m, 2H), 4.80 (s, 1H), 3.97 (d, J=2.2 Hz, 3H), 3.82 (s, 1H), 3.62 (d, J=11.3 Hz, 1H), 3.36 (s, 1H), 3.24 (s, 1H), 2.82 (t, J=10.2 Hz, 1H), 2.15 (s, 1H), 1.80 (d, J=40.9 Hz, 3H), 1.48-1.42 (m, 3H), 1.42-1.34 (m, 3H), 0.73 (dd, J=6.1, 2.3 Hz, 3H). 
     For Example 243: LCMS (C 23 H 29 FN 8 O 2 ) (ES, m/z): 469 [M+H] + .  1 H NMR (499 MHz, DMSO-d 6 ) δ 7.93-7.83 (m, 1H), 7.71 (s, 2H), 7.38 (s, 1H), 7.22-7.10 (m, 2H), 4.81 (s, 1H), 4.01-3.95 (m, 3H), 3.82 (s, 1H), 3.62 (d, J=10.8 Hz, 1H), 3.37 (s, 1H), 3.24 (s, 1H), 2.82 (t, J=11.3 Hz, 1H), 2.55 (d, J=9.7 Hz, 1H), 2.15 (s, 1H), 1.90-1.67 (m, 3H), 1.45 (s, 3H), 1.39 (s, 3H), 0.76-0.67 (m, 3H). 
     The example compounds of the invention in the following Table 32 were prepared in a manner similar to that described for the preparation of Example 242 and Example 243 from the appropriate starting amine and aryl halide, where the resulting isomeric mixture of the corresponding final compounds were separated by SFC. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 32 
               
               
                   
               
               
                   
                   
                   
                 Observed 
               
               
                   
                 Structure 
                   
                 m/z  
               
               
                 Example 
                 Name 
                 SFC Conditions 
                 [M + H] +   
               
               
                   
               
             
            
               
                 244 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiral Technologies IA 21 × 250 mm column  with 40% (MeOH  w/0.1% NH 4 OH  modifier) as co- solvent 
                 469 
               
               
                   
               
               
                   
                 (R or S)-3-(4-((R)-3-(5-amino-9-fluoro-7-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)- 
                   
                   
               
               
                   
                 1H-pyrazol-1-yl)-3-methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 245 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies IA 21 × 250 mm column  with 40% (MeOH  w/0.1% NH 4 OH  modifier) as co- solvent 
                 469 
               
               
                   
               
               
                   
                 (S or R)-3-(4-((R)-3-(5-amino-9-fluoro-7-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)- 
                   
                   
               
               
                   
                 1H-pyrazol-1-yl)-3-methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 246 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; ES Industries CCA 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 457 
               
               
                   
               
               
                   
                 (R or S)-3-(4-((R)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)- 
                   
                   
               
               
                   
                 1H-pyrazol-1-yl)-3-methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 247 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; ES Industries CCA 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 457 
               
               
                   
               
               
                   
                 (S or R)-3-(4-((R)-3-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)piperidin-1-yl)- 
                   
                   
               
               
                   
                 1H-pyrazol-1-yl)-3-methylbutan-2-ol 
               
               
                   
               
            
           
         
       
     
     Example 248 and Example 249: 2-(4-((2S,5R or 2R,5S)-5-(5-amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol and 2-(4-((2R,5S or 2S,5R)-5-(5-amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Step 1: 2-(4-(5-(5-((2,4-dimethoxybenzyl)amino)-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol 
     To a solution of 1-(1-(1,3-dihydroxy-2-methylpropan-2-yl)-1H-pyrazol-4-yl)-6-methylpiperidine-3-carbohydrazide (105 mg, 0.336 mmol) (Intermediate 166) in DMF (1 mL) was added AcOH (9.63 μl, 0.168 mmol), 2-((((3,4-dimethylbenzyl)imino)methylene)amino)-3,5-difluorobenzonitrile (Intermediate 37) (100 mg, 0.336 mmol) at 50° C. under an atmosphere of nitrogen. The mixture was stirred and heated at 50° C. for 16 h. The mixture was cooled, diluted with water (20 mL), and extracted with EtOAc (2×20 mL). The organic layer was dried over anhydrous Na 2 SO 4 , filtered and the solvents were evaporated. The resulting residue was purified by preparative silica gel TLC with 10% MeOH in DCM as eluent to afford 2-(4-(5-(5-((2,4-dimethoxybenzyl)amino)-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol. LCMS (C 31 H 36 F 2 N 8 O 4 ) (ES, m/z): 623 [M+H] + . 
     Step 2: 2-(4-(5-(5-amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3-hydroxy-2-methylpropyl 2,2,2-trifluoroacetate To a solution of 2-(4-(5-(5-((2,4-dimethoxybenzyl)amino)-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol (60 mg, 0.096 mmol) in DCM (2 mL) was added TFA (2.0 mL, 26 mmol) at 10° C. under a nitrogen atmosphere. The mixture was stirred at 10° C. for 16 h. The solvents were evaporated to afford the crude product of 2-(4-(5-(5-amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3-hydroxy-2-methylpropyl 2,2,2-trifluoroacetate, which was used in the next step without any further purification. LCMS (C 24 H 25 F 5 N 8 O 3 ) (ES, m/z): 569 [M+H] + . 
     Step 3: 2-(4-((2S,5R or 2R,5S)-5-(5-amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol and 2-(4-((2R,5S or 2S,5R)-5-(5-amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol 
     To a solution of 2-(4-(5-(5-amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3-hydroxy-2-methylpropyl 2,2,2-trifluoroacetate (40 mg, 0.070 mmol) in MeOH (2 mL) was added Na 2 CO 3  (7.5 mg, 0.070 mmol) at 10° C. under a nitrogen atmosphere. The mixture was stirred at 10° C. for 1 h. The solvents were evaporated to afford a mixture of isomers. The mixture was submitted for SFC chiral separation (Chiralpak AD-3 4.6×150 mm column with 5-40% (MeOH w/0.05% DEA modifier) as co-solvent), to afford 2-(4-((2S,5R or 2R,5S)-5-(5-amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol (Example 248, peak 1) and 2-(4-((2R,5S or 2S,5R)-5-(5-amino-7,9-difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol (Example 249, peak 2). 
     For Example 248: LCMS (C 22 H 26 F 2 N 8 O 2 ) (ES, m/z): 473 [M+H] + .  1 H NMR (500 MHz, methanol-d 4 ) δ=7.71 (br dd, J=1.3, 6.8 Hz, 1H), 7.38-7.26 (m, 2H), 7.22 (s, 1H), 3.78-3.68 (m, 4H), 3.64 (br dd, J=3.8, 6.1 Hz, 1H), 3.41 (br d, J=8.2 Hz, 1H), 2.96 (s, 1H), 2.82 (s, 1H), 2.06-1.99 (m, 2H), 1.98 (s, 1H), 1.73 (br dd, J=3.1, 12.7 Hz, 1H), 1.42 (s, 3H), 1.03 (d, J=6.7 Hz, 3H). 
     For Example 249: LCMS (C 22 H 26 F 2 N 8 O 2 ) (ES, m/z): 473 [M+H] + .  1 H NMR (500 MHz, methanol-d 4 ) δ=7.84 (br s, 1H), 7.53-7.41 (m, 2H), 7.36 (br s, 1H), 3.93-3.79 (m, 4H), 3.78 (br s, 1H), 3.55 (br s, 1H), 2.22 (br s, 1H), 2.18-2.09 (m, 2H), 1.87 (br d, J=10.1 Hz, 1H), 1.54 (s, 3H), 1.31 (s, 2H), 1.16 (d, J=6.6 Hz, 3H). 
     The example compounds of the invention in the following Table 33 were prepared in a manner similar to that described for the preparation of Example 248 and Example 249 from the appropriate starting hydrazide, where the resulting isomeric mixture of the corresponding final compounds were separated by SFC. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 33 
               
               
                   
               
               
                   
                   
                   
                 Observed 
               
               
                   
                 Structure 
                   
                 m/z 
               
               
                 Example 
                 Name 
                 SFC Conditions 
                 [M + H] +   
               
               
                   
               
             
            
               
                 250 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak  AD-3 4.6 × 150 mm column with 5-40% (MeOH w/0.05% DEA modifier) as  co-solvent 
                 485 
               
               
                   
               
               
                   
                 2-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin- 
                   
                   
               
               
                   
                 1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol 
                   
                   
               
               
                   
               
               
                 251 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak  AD-3 4.6 × 150 mm column with 5-40% (MeOH w/0.05% DEA modifier) as  co-solvent.  
                 485 
               
               
                   
               
               
                   
                 2-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin- 
                   
                   
               
               
                   
                 1-yl)-1H-pyrazol-1-yl)-2-methylpropane-1,3-diol 
                   
                   
               
               
                   
               
               
                 252 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralcel  OJ-3 4.6 × 100 mm  column with 5-40%  (EtOH w/0.05%  DEA modifier) as  co-solvent. Then Peak 1; Chiralpak  AD-3 4.6 × 150 mm column with 40% (MeOH w/0.05% DEA modifier) as  co-solvent.  
                 485 
               
               
                   
               
               
                   
                 (R or S)-2-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)- 
                   
                   
               
               
                   
                 1H-pyrazol-1-yl)-2-methylpropane-1,2-diol 
                   
                   
               
               
                   
               
               
                 253 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralcel  OJ-3 4.6 × 100 mm  column with 5-40%  (EtOH w/0.05%  DEA modifier) as  co-solvent. Then Peak 2; Chiralpak  AD-3 4.6 × 150 mm column with 40% (MeOH w/0.05% DEA modifier) as  co-solvent.  
                 485 
               
               
                   
               
               
                   
                 (S or R)-2-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)- 
                   
                   
               
               
                   
                 1H-pyrazol-1-yl)-2-methylpropane-1,2-diol 
                   
                   
               
               
                   
               
               
                 254 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Chiralcel  OJ-3 4.6 × 100 mm column with 5-40% (EtOH w/0.05% DEA modifier) as  co-solvent. 
                 485 
               
               
                   
               
               
                   
                 (R or S)-2-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)- 
                   
                   
               
               
                   
                 1H-pyrazol-1-yl)-2-methylpropane-1,2-diol 
                   
                   
               
               
                   
               
               
                 255 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 4; Chiralcel  OJ-3 4.6 × 100 mm column with 5-40% (EtOH w/0.05% DEA modifier) as  co-solvent. 
                 485 
               
               
                   
               
               
                   
                 (S or R)-2-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1-yl)- 
                   
                   
               
               
                   
                 1H-pyrazol-1-yl)-2-methylpropane-1,2-diol 
                   
                   
               
               
                   
               
               
                 256 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Cellulose 2 4.6 × 100 mm column with 40% (MeOH  w/0.05% DEA  modifier) as co- solvent. Then Peak 1; Chiralpak  AS-3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co- solvent. 
                 473 
               
               
                   
               
               
                   
                 (R or S)-2-(4-((2S,5R or 2R,5S)-5-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1- 
                   
                   
               
               
                   
                 yl)-1H-pyrazol-1-yl)-2-methylpropane-1,2-diol 
                   
                   
               
               
                   
               
               
                 257 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Cellulose 2 4.6 × 100 mm column with 40% (MeOH  w/0.05% DEA  modifier) as co- solvent. Then Peak 2; Chiralpak  AS-3 4.6 × 150 mm column with 5-40% (IPA w/0.05% DEA modifier) as co- solvent. 
                 473 
               
               
                   
               
               
                   
                 (S or R)-2-(4-((2S,5R or 2R,5S)-5-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1- 
                   
                   
               
               
                   
                 yl)-1H-pyrazol-1-yl)-2-methylpropane-1,2-diol 
                   
                   
               
               
                   
               
               
                 258 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 4; Cellulose 2 4.6 × 100 mm column with 40% (MeOH  w/0.05% DEA  modifier) as co- solvent.  
                 473 
               
               
                   
               
               
                   
                 (R or S)-2-(4-((2R,5S or 2S,5R)-5-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1- 
                   
                   
               
               
                   
                 yl)-1H-pyrazol-1-yl)-2-methylpropane-1,2-diol 
                   
                   
               
               
                   
               
               
                 259 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 5; Cellulose 2 4.6 × 100 mm column with 40% (MeOH  w/0.05% DEA  modifier) as co- solvent.  
                 473 
               
               
                   
               
               
                   
                 (S or R)-2-(4-((2R,5S or 2S,5R)-5-(5-amino-7,9-difluoro- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2-methylpiperidin-1- 
                   
                   
               
               
                   
                 yl)-1H-pyrazol-1-yl)-2-methylpropane-1,2-diol 
                   
                   
               
               
                   
               
               
                 260 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak  AS-3 4.6 × 100 mm column with 5-40% (MeOH w/0.05% DEA modifier) as co- solvent. 
                 487 
               
               
                   
               
               
                   
                 (R or S)-2-(4-((2R,5S or 2S,5R)-5-(5-amino-7,9- 
                   
                   
               
               
                   
                 difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropane-1,2-diol 
                   
                   
               
               
                   
               
               
                 261 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak  AS-3 4.6 × 100 mm column with 5-40% (MeOH w/0.05% DEA modifier) as  co-solvent. 
                 487 
               
               
                   
               
               
                   
                 (S or R)-2-(4-((2R,5S or 2S,5R)-5-(5-amino-7,9- 
                   
                   
               
               
                   
                 difluoro-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-2- 
                   
                   
               
               
                   
                 methylpropane-1,2-diol 
                   
                   
               
               
                   
               
               
                 262 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; ES Industries CC4 21 × 250 mm column with 40% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. 
                 468 
               
               
                   
               
               
                   
                 2-((3R,6S or 3S,6R)-1-(1-(2-amino-2-methylpropyl)-1H-pyrazol-4- 
                   
                   
               
               
                   
                 yl)-6-methylpiperidin-3-yl)-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
                   
               
               
                   
               
               
                 263 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; ES Industries CC4 21 × 250 mm column with 40% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. 
                 468 
               
               
                   
               
               
                   
                 2-((3S,6R or 3R,6S)-1-(1-(2-amino-2-methylpropyl)-1H-pyrazol-4- 
                   
                   
               
               
                   
                 yl)-6-methylpiperidin-3-yl)-9-fluoro-8-methoxy- 
                   
                   
               
               
                   
                 [1,2,4]triazolo[1,5-c]quinazolin-5-amine 
                   
                   
               
               
                   
               
               
                 264 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; ES Industries CCA 21 × 250 mm column with 25% (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 (R or S)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3- 
                   
                   
               
               
                   
                 methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 265 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2 (mixture of Example 265 and Example 266); ES Industries CCA 21 ×  250 mm column with 25% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. Then Peak 1; Lux-4 21 × 250  mm column with 35% (MeOH w/0.1% NH 4 OH modifier) 
                 483 
               
               
                   
               
               
                   
                 (S or R)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3- 
                   
                   
               
               
                   
                 methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 266 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2 (mixture of Example 265 and Example 266); ES Industries CCA 21 ×  250 mm column with 25% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. Then Peak 2; Lux-4 21 × 250  mm column with 35%  (MeOH w/0.1% NH 4 OH modifier) 
                 483 
               
               
                   
               
               
                   
                 (R or S)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)- 
                   
                   
               
               
                   
                 2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3- 
                   
                   
               
               
                   
                 methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 267 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; ES Industries  CCA 21 × 250 mm  column with 25%  (MeOH w/0.1% NH 4 OH modifier) as co-solvent 
                 483 
               
               
                   
               
               
                   
                 (S or R)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 8-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)- 
                   
                   
               
               
                   
                 2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3- 
                   
                   
               
               
                   
                 methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 268 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1 (mixture of Example 268 and Example 269); Chiral Technologies OJ-H  21 × 250 mm column  with 10% (MeOH  w/0.1% NH 4 OH  modifier) as co- solvent. Then peak 1;  ID 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. 
                 483 
               
               
                   
               
               
                   
                 (R or S)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)- 
                   
                   
               
               
                   
                 2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3- 
                   
                   
               
               
                   
                 methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 269 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1 (mixture of Example 268 and Example 269); Chiral Technologies OJ-H  21 × 250 mm column  with 10% (MeOH  w/0.1% NH 4 OH  modifier) as co- solvent. Then peak 2;  ID 21 × 250 mm column with 35% (MeOH w/0.1% NH 4 OH modifier) as co-solvent. 
                 483 
               
               
                   
               
               
                   
                 (S or R)-3-(4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)- 
                   
                   
               
               
                   
                 2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3- 
                   
                   
               
               
                   
                 methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 270 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies OJ-H  21 × 250 mm column  with 10% (MeOH  w/0.1% NH 4 OH  modifier) as co- solvent. 
                 483 
               
               
                   
               
               
                   
                 (R or S)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)- 
                   
                   
               
               
                   
                 2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3- 
                   
                   
               
               
                   
                 methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 271 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Chiral Technologies OJ-H  21 × 250 mm column  with 10% (MeOH  w/0.1% NH 4 OH  modifier) as co- solvent. 
                 483 
               
               
                   
               
               
                   
                 (S or R)-3-(4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro- 
                   
                   
               
               
                   
                 7-methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)- 
                   
                   
               
               
                   
                 2-methylpiperidin-1-yl)-1H-pyrazol-1-yl)-3- 
                   
                   
               
               
                   
                 methylbutan-2-ol 
                   
                   
               
               
                   
               
               
                 272 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 1; Chiralpak AS- 3 4.6 × 150 mm column with 5-40% (EtOH w/0.05% DEA modifier) as co- solvent. 
                 467 
               
               
                   
               
               
                   
                 1-((4-((2S,5R or 2R,5S)-5-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)methyl)cyclopropan-1-ol 
                   
                   
               
               
                   
               
               
                 273 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiralpak AS- 3 4.6 × 150 mm column with 5-40% (EtOH w/0.05% DEA modifier) as co- solvent. 
                 467 
               
               
                   
               
               
                   
                 1-((4-((2R,5S or 2S,5R)-5-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-2- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)methyl)cyclopropan-1-ol 
                   
                   
               
               
                   
               
               
                 274 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 2; Chiral Technologies AD-H 21 × 250 mm column with 30% (MeOH w/ 0.1% NH 4 OH modifier) as co- solvent. 
                 483 
               
               
                   
               
               
                   
                 1-((4-((3R,5S or 3S,5R)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)-2-methylpropan-2-ol 
                   
                   
               
               
                   
               
               
                 275 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Peak 3; Chiral Technologies AD-H 21 × 250 mm column with 30% (MeOH w/ 0.1% NH 4 OH modifier) as co- solvent. 
                 483 
               
               
                   
               
               
                   
                 1-(4-((3S,5R or 3R,5S)-3-(5-amino-9-fluoro-8- 
                   
                   
               
               
                   
                 methoxy-[1,2,4]triazolo[1,5-c]quinazolin-2-yl)-5- 
                   
                   
               
               
                   
                 methylpiperidin-1-yl)-5-methyl-1H-pyrazol-1- 
                   
                   
               
               
                   
                 yl)-2-methylpropan-2-ol 
               
               
                   
               
            
           
         
       
     
     Biological Assays 
     The IC 50  values reported for each of the compounds of the invention shown in the table below were measured in accordance with the methods described below. 
     The A2a receptor affinity binding assay measured the amount of binding of a tritiated ligand with high affinity for the A2a adenosine receptor to membranes made from HEK293 or CHO cells recombinantly expressing the human A2a adenosine receptor, in the presence of varying concentrations of a compound of the invention. In each assay, the tested compounds of the invention were solubilized in 100% DMSO and further diluted in 100% DMSO to generate, typically, a 10-point titration at half-log intervals such that the final assay concentrations did not exceed 10 μM of compound or 1% DMSO. 
     Measurement of A2a Binding Affinity Using Radioligand Binding 
     148 μL (5 μg/mL) membranes (Perkin Elmer, Cat. No. RBHA2aM400UA) and 2 μL compounds of the invention to be tested (test compound) were transferred to individual wells of a 96-well polypropylene assay plate and incubated for 15 to 30 minutes at room temperature. [ 3 H] SCH58261 ((7-(2-phenylethyl)-5-amino-2-(2-furyl)-pyrazolo-[4,3-e]-1,2,4-triazolo[1,5-c]pyrimidine)) was diluted in assay buffer (50 mM Tris pH 7.4, 10 mM MgCl 2 , 0.005% Tween20) to a concentration of 4 nM and 50 μL transferred to each well of the assay plate. To define total and non-specific binding, wells containing 1% DMSO and 1 μM ZM241385 (Tocris Bioscience, Cat. No. 1036) respectively, were also included. The assay plate was incubated at room temperature for 60 minutes with agitation. Using a FilterMate Harvester® (Perkin Elmer), the contents of the assay plate were filtered through a UniFilter-96® PEI coated plate (Perkin Elmer Cat. No. 6005274 or 6005277). Filtering was achieved by aspirating the contents of the assay plate for 5 seconds, then washing and aspirating the contents three times with ice-cooled wash buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl) and allowing the vacuum manifold to dry the plate for 30 seconds. The filter plate was incubated for at least 1 hour at 55° C. and allowed to dry. The bottom of the filter plate was sealed with backing tape. 40 μL Ultima Gold™ (Perkin Elmer, Cat. No. 6013329) was added to each well of the filter plate and the top of the plate was sealed with TopSeal-A PLUS® clear plate seal (Perkin Elmer, Cat. No. 6050185). The plate was incubated for at least 20 minutes, and then the amount of radioactivity remaining in each well was determined using a TopCount® (Perkin Elmer) scintillation counter. After normalization to total and non-specific binding, the percent effect at each compound concentration was calculated. The plot of percent effect versus the log of compound concentration was analyzed electronically using a 4-parameter logistic fit based on the Levenberg-Marquardt algorithm to generate IC 50  values. 
     Measurement of A2b Binding Affinity 
     The reported affinity of the compounds of the invention for the human A2b adenosine receptor was determined experimentally using a radioligand filtration binding assay. This assay measures the amount of binding of a tritiated proprietary A2b receptor antagonist, in the presence and absence of a compound of the invention, to membranes made from HEK293 cells recombinantly expressing the human A2b adenosine receptor (Perkin Elmer, Cat. No. ES-013-C). 
     To perform the assay, compounds of the invention to be tested were first solubilized in 100% DMSO and further diluted in 100% DMSO to generate, typically, a 10-point titration at half-log intervals such that the final assay concentrations did not exceed 10 μM of compound or 1% DMSO. 148 μL (135 μg/mL) membranes and 2 μL test compounds were transferred to individual wells of a 96-well polypropylene assay plate and incubated for 15 to 30 minutes at room temperature with agitation. Tritiated radioligand was diluted to a concentration of 14 nM in assay buffer (phosphate buffered saline without Magnesium and Calcium, pH 7.4; GE Healthcare Life Sciences, Cat. No. SH30256.01) and then 50 μL of the solution were transferred to each well of the assay plate. To define total and non-specific binding, wells containing 1% DMSO and 20 μM N-ethylcarboxamidoadenosine (Tocris Bioscience, Cat. No. 1691) respectively, were also included. The wells of the assay plate were incubated at room temperature for 60 minutes with agitation, then filtered using a FilterMate Harvester® (Perkin Elmer) or similar equipment through a UniFilter-96® PEI coated plate (Perkin Elmer Cat. No. 6005274 or 6005277). Filtering was achieved by aspirating the contents of the assay plate for 5 seconds, then washing and aspirating the contents three times with ice-cooled wash buffer (assay buffer supplemented with 0.0025% Brij58) and allowing the vacuum manifold to dry the plate for 30 seconds. The filter plate was incubated for at least 1 hour at 55° C. and allowed to dry. The bottom of the filter plate was then sealed with backing tape. 40 μL Ultima Gold™ (Perkin Elmer, Cat. No. 6013329) was added to each well of the filter plate and the top of the plate was sealed with TopSeal-A PLUS® clear plate seal (Perkin Elmer, Cat. No. 6050185). The plates were then incubated for at least 20 minutes, and then the amount of radioactivity remaining in each well was determined using a TopCount® (Perkin Elmer) scintillation counter. After normalization to total and non-specific binding, the percent effect at each compound concentration was calculated. The plot of percent effect versus the log of compound concentration was analyzed electronically using a 4-parameter logistic fit based on the Levenberg-Marquardt algorithm to generate IC 50  values. 
                                     Example   A 2A  IC 50  binding (nM)   A 2B  IC 50  binding (nM)                                            1   7.5   554.2       2   3.7   9.4       3   1.5   180.4       4   4.6   2.6       5   3.3   1.6       6   2.0   85.9       7   3.7   104.1       8   4.5   149.3       9   0.5   136.0       10   3.5   63.4       11   0.5   56.0       12   1.7   75.0       13   0.7   72.6       14   3.0   224.9       15   38.4   905.2       16   0.8   426.0       17   1.7   433.9       18   0.7   13.5       19   0.7   87.3       20   5.8   163       21   0.3   514.6       22   8.6   27.6       23   2.0   15.9       24   13.8   480.5       25   0.6   61.7       26   3.9   48.7       27   1.8   860.8       28   2.8   76.3       29   1.5   301.1       30   0.4   97.9       31   1.7   94.8       32   0.5   6.9       33   3.6   17.5       34   1.4   29.7       35   0.6   12.2       36   1.6   93.0       37   1.1   468.1       38   16.9   182.4       39   1.1   43.3       40   1.2   9.2       41   1.2   42.8       42   4.3   475.7       43   0.7   3.4       44   1.3   20.4       45   1.8   150.4       46   0.6   86.4       47   8.2   284.2       48   0.6   17.6       49   0.3   2.7       50   18.3   1889       51   21.0   2079       52   18.3   3006       53   44.1   4239       54   3.3   194.6       55   10.6   500.2       56   1.8   846.5       57   48.3   85.6       58   13.6   136.2       59   67.0   4744       60   31.4   5862       61   5.0   99.0       62   146.1   382.8       63   242.2   2431       64   1.0   555.3       65   1.2   227.9       66   0.6   957.1       67   44% Inh.   31% Inh.           @ 1000 nM   @ 10000 nM       68   181.4   31% Inh.               @ 10000 nM       69   2.6   561.4       70   0.6   786.5       71   35.6   43% Inh.               @ 10000 nM       72   2.0   484       73   436.0   5124       74   0.9   153.3       75   56.9   54% Inh.               @ 10000 nM       76   256.4   34% Inh.               @ 10000 nM       77   0.4   107.9       78   0.9   233.7       79   16.0   3141       80   11.6   404.5       81   121.1   6582       82   4.9   63.0       83   2.7   113.0       84   2.3   116.6       85   5.2   192.1       86   0.3   164.7       87   0.4   133.8       88   0.1   1.8       89   0.2   3.4       90   1.2   29.0       91   44.7   4624       92   10.9   602.3       93   5.8   314.6       94   7.8   752.0       95   205   5041       96   0.7   24.6       97   0.6   33.3       98   0.9   116.4       99   4.4   230.9       100   1.4   4.8       101   32.7   390.0       102   103.5   538.7       103   0.5   136.7       104   17.7   766.1       105   328.6   743.0       106   316.4   429.6       107   126.9   1522       108   36% Inh.   8474           @ 1000 nM           109   0.7   7.8       110   0.2   1.8       111   0.7   16.5       112   0.4   7.9       113   58.1   470.5       114   50.6   2905       115   2.9   895.1       116   207.4   35% Inh.               @ 10000 nM       117   0.2   13.0       118   1.9   386.7       119   51.6   1302       120   0.6   7.8       121   55.4   5344       122   368.7   2058       123   0.3   422.2       124   0.3   1.7       125   86.4   2501       126   2.6   2158       127   120.5   1158       128   0.3   2.4       129   4.1   469.9       130   1.2   78.7       131   11.8   66.0       132   6.7   242.6       133   4.3   558.5       134   123.6   4288       135   14.1   583.3       136   1.0   7.9       137   79.3   924.5       138   13.2   2257       139   16.8   2824       140   0.8   2723       141   24.1   1756       142   32% Inh.    7937           @ 1000 nM           143   984.1   7557       144   30% Inh.    7143           @ 1000 nM           145   32% Inh.    8046           @ 1000 nM           146   2.1   137.1       147   870.2   6333       148   385.1   4429       149   1.8   96.6       150   1.4   180.6                    
Measurement of A 2A  and A 2B  Antagonism in cAMP Cell-Based Assay
 
     The ability of compounds to antagonize human A 2A  and A 2B  adenosine receptors was determined using a kit to measure changes in intracellular cyclic AMP levels (LANCE cAMP 384 Kit, Perkin Elmer, Cat. No. AD0264). HEK293 cells recombinantly expressing either human A 2A  or A 2B  receptors, previously frozen in Recovery Medium (Life Technologies, Cat. No. 12648-010) were thawed and diluted into stimulation buffer (HBSS (Hyclone SH 30268.01), 5 mM HEPES (Gibco 15630-106), 200 nM rolipram (Tocris, Cat. No. 0905), and 1.5% (V/v) BSA stabilizer (kit component). The cell suspension was centrifuged at 200×g for 10 min and then resuspended in stimulation buffer, supplemented with a 1:10 000 dilution of Alexa Fluor 647 anti-cAMP antibody, to a density of 6.0×10 5  cells/mL. A Labcyte Echo 550 acoustic dispenser was used to transfer up to 25 nL of test compound dissolved in DMSO into the wells of a dry Optiplate-384 plate (Perkin Elmer, Cat. No. 6008289). All subsequent liquid additions were performed using a multichannel pipettor. Next, 5 μL of the cell suspension was added to the wells of the Optiplate-384 and incubated for 30 min. at 37° C. and 5% CO 2  in a humidified environment. After this time 5 μL of either 300 nM or 600 nM adenosine (Sigma Cat. No. A9251) for A 2A  and A 2B  respectively was added and incubated for 30 minutes at 37° C. and 5% CO 2  in a humidified environment. At this time detection mix was prepared by combining the LANCE Eu-W8044 labeled streptavidin and Biotin-cAMP in detection buffer according to the manufacturers protocol. 10 μL of the detection mix was added to each well of the Optiplate-384 which was covered with a plate seal and incubated under ambient conditions for 2 hours prior to reading the plate using an Envision (Perkin Elmer, Waltham, Mass.) multimode plate reader. Data was normalized by defining minimal effect as stimulation in the presence of 0.25% (v/v) DMSO and maximal effect as stimulation in the presence of 1 μM ZM241385 (Cayman, Cat. No. 1036). Curve fitting of the percent effect data versus the log of compound concentration used a 4-parameter concentration response curve fitting algorithm to calculate IC 50  values. Compound concentrations tested were 10,000, 3,333, 1,111, 370.4, 123.4, 41.2, 13.7, 4.6, 1.5 and 0.5 nM with 0.25% residual DMSO. 
     
       
         
           
               
               
               
             
               
                   
               
               
                 Example 
                 A 2A  IC 50  c AMP (nM) 
                 A 2B  IC 50  c AMP (nM) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 151 
                 3.8 
                 56.9 
               
               
                 152 
                 5.9 
                 371 
               
               
                 153 
                 7.4 
                 719.8 
               
               
                 154 
                 1.2 
                 974.1 
               
               
                 155 
                 23.9 
                 1436 
               
               
                 156 
                 9.5 
                 358.1 
               
               
                 157 
                 33.4 
                 4481 
               
               
                 158 
                 1.1 
                 32.9 
               
               
                 159 
                 1.0 
                 29.3 
               
               
                 160 
                 8.7 
                 647.8 
               
               
                 161 
                 38.9 
                 3268 
               
               
                 162 
                 0.7 
                 20.7 
               
               
                 163 
                 36.0 
                 5306 
               
               
                 164 
                 1.9 
                 47.7 
               
               
                 165 
                 0.8 
                 6.4 
               
               
                 166 
                 20.0 
                 2769 
               
               
                 167 
                 0.6 
                 46.0 
               
               
                 168 
                 23.9 
                 4723 
               
               
                 169 
                 96.5 
                 3382 
               
               
                 170 
                 0.8 
                 14.1 
               
               
                 171 
                 1.2 
                 11.3 
               
               
                 172 
                 60.5 
                 14% Inh. 
               
               
                   
                   
                 @ 10000 nM 
               
               
                 173 
                 20.7 
                 2022 
               
               
                 174 
                 0.6 
                 30.7 
               
               
                 175 
                 30.2 
                 2004 
               
               
                 176 
                 0.9 
                 10.2 
               
               
                 177 
                 154 
                 3957 
               
               
                 178 
                 0.6 
                 11.5 
               
               
                 179 
                 4.0 
                 335.5 
               
               
                 180 
                 54.0 
                 5342 
               
               
                 181 
                 16.0 
                 5099 
               
               
                 182 
                 361.9 
                 &gt;10000 
               
               
                 183 
                 2.0 
                 90.6 
               
               
                 184 
                 4.9 
                 345.5 
               
               
                 185 
                 1.1 
                 49.8 
               
               
                 186 
                 1.0 
                 25.8 
               
               
                 187 
                 2.6 
                 95.8 
               
               
                 188 
                 1.5 
                 53.1 
               
               
                 189 
                 0.5 
                 53.4 
               
               
                 190 
                 0.8 
                 55.9 
               
               
                 191 
                 0.9 
                 92.4 
               
               
                 192 
                 0.8 
                 72.4 
               
               
                 193 
                 1.3 
                 250.7 
               
               
                 194 
                 1.7 
                 318.4 
               
               
                 195 
                 3.4 
                 389.5 
               
               
                 196 
                 3.7 
                 474.9 
               
               
                 197 
                 6.4 
                 93.0 
               
               
                 198 
                 4.3 
                 52.4 
               
               
                 199 
                 2.5 
                 97.5 
               
               
                 200 
                 2.5 
                 102.5 
               
               
                 201 
                 1.9 
                 38.4 
               
               
                 202 
                 1.4 
                 62.4 
               
               
                 203 
                 7.7 
                 53.2 
               
               
                 204 
                 7.8 
                 87.7 
               
               
                 205 
                 7.0 
                 1146 
               
               
                 206 
                 13.0 
                 4314 
               
               
                 207 
                 0.9 
                 10.37 
               
               
                 208 
                 0.7 
                 2799 
               
               
                 209 
                 0.7 
                 634.9 
               
               
                 210 
                 16.2 
                 35% Inh. 
               
               
                   
                   
                 @ 10000 nM 
               
               
                 211 
                 89.3 
                 &gt;10000 
               
               
                 212 
                 121.7 
                 27% Inh. 
               
               
                   
                   
                 @ 10000 nM 
               
               
                 213 
                 330.8 
                 &gt;10000 
               
               
                 214 
                 0.5 
                 34.09 
               
               
                 215 
                 0.4 
                 27.5 
               
               
                 216 
                 1.0 
                 159.7 
               
               
                 217 
                 1.1 
                 161.3 
               
               
                 218 
                 235.8 
                 5775 
               
               
                 219 
                 246.7 
                 27% Inh. 
               
               
                   
                   
                 @ 10000 nM 
               
               
                 220 
                 20.4 
                 30% Inh. 
               
               
                   
                   
                 @ 10000 nM 
               
               
                 221 
                 0.5 
                 63 
               
               
                 222 
                 34.6 
                 3277 
               
               
                 223 
                 0.7 
                 46 
               
               
                 224 
                 0.4 
                 13.1 
               
               
                 225 
                 0.4 
                 12.1 
               
               
                 226 
                 17.6 
                 1611 
               
               
                 227 
                 40.2 
                 5071 
               
               
                 228 
                 0.8 
                 16.1 
               
               
                 229 
                 1.0 
                 6.6 
               
               
                 230 
                 32.9 
                 723.7 
               
               
                 231 
                 121.5 
                 3845 
               
               
                 232 
                 1.3 
                 12.7 
               
               
                 233 
                 0.7 
                 5.6 
               
               
                 234 
                 46.8 
                 1195 
               
               
                 235 
                 438.8 
                 4450 
               
               
                 236 
                 1.0 
                 5.1 
               
               
                 237 
                 0.8 
                 8.7 
               
               
                 238 
                 21.8 
                 2438 
               
               
                 239 
                 75.1 
                 10% Inh. 
               
               
                   
                   
                 @ 10000 nM 
               
               
                 240 
                 1.2 
                 22.6 
               
               
                 241 
                 1.3 
                 21.7 
               
               
                 242 
                 2.0 
                 34.9 
               
               
                 243 
                 2.6 
                 50.7 
               
               
                 244 
                 0.9 
                 18.8 
               
               
                 245 
                 2.0 
                 25.8 
               
               
                 246 
                 3.2 
                 21.3 
               
               
                 247 
                 4.5 
                 21.8 
               
               
                 248 
                 1.4 
                 8.4 
               
               
                 249 
                 26.9 
                 197.1 
               
               
                 250 
                 0.7 
                 4.9 
               
               
                 251 
                 39.5 
                 387.1 
               
               
                 252 
                 95.0 
                 8157 
               
               
                 253 
                 123.1 
                 &gt;10000 
               
               
                 254 
                 1.5 
                 10. 
               
               
                 255 
                 2.6 
                 28.8 
               
               
                 256 
                 240.7 
                 3892 
               
               
                 257 
                 540.6 
                 25% Inh. 
               
               
                   
                   
                 @ 10000 nM 
               
               
                 258 
                 2.3 
                 8.1 
               
               
                 259 
                 2.3 
                 11.8 
               
               
                 260 
                 1.7 
                 10.0 
               
               
                 261 
                 86.6 
                 1473 
               
               
                 262 
                 2.2 
                 23.9 
               
               
                 263 
                 26.0 
                 510.8 
               
               
                 264 
                 3.0 
                 14.3 
               
               
                 265 
                 1.3 
                 4.5 
               
               
                 266 
                 64.2 
                 579.6 
               
               
                 267 
                 175.1 
                 1215 
               
               
                 268 
                 0.7 
                 4.7 
               
               
                 269 
                 1.2 
                 6.4 
               
               
                 270 
                 5.2 
                 30.3 
               
               
                 271 
                 21.4 
                 241.8 
               
               
                 272 
                 43.6 
                 5800 
               
               
                 273 
                 1.2 
                 26.7 
               
               
                 274 
                 0.7 
                 70.5 
               
               
                 275 
                 15.6 
                 2468