Patent Publication Number: US-2020281208-A1

Title: Use of crystalline forms of copper salicylate for the control of phytopathogenic bacteria

Description:
The present invention relates to the use of crystalline forms of copper salicylate having a bactericidal activity, and agronomical compositions containing them, for the control of phytopathogenic bacteria. 
     STATE OF THE ART 
     Bacterioses are plant diseases caused by phytopathogenic bacteria. The bacteria can strike any type of plant, even if the most common bacterial diseases relate to some ornamental species, fruit trees such as olive trees, kiwi trees and apricot trees, as well as tomatoes, citrus fruits and garlic. 
     Unlike fungi, these infectious agents are not capable of actively penetrating the walls of the epidermal tissues of plants. Their entry and attack consequently occurs in points where the barriers formed by the epidermal tissues of the plants, are naturally or occasionally interrupted, such as, for example, under excessive wetting conditions of the locations or in the case of incorrect grafting. 
     Bacterioses can not only cause the death of the plant, but also a whole series of correlated diseases, such as tumours, that lead to the drying of the plant structure and subsequently to the death of the species affected. In order to combat the spreading of the disease, symptomatic plants are eradicated and various preventive interventions are effected with fertilizers and/or bio-stimulants. 
     Very frequently, however, these interventions are not sufficient for effectively controlling the disease, thus making it necessary to combat bacterial diseases by the application of specific agrochemicals. For this purpose, recalling that in Italy, since 1971, resort can no longer be made to the use of antibiotics, such as streptomycin or tetracycline, plants are normally treated with resistance-inducer active principles and/or with classical cupric compounds, such as copper hydroxide, possibly combined with some carboxylic acids, as indicated in patent application US2009/136581, which however can prove to be phytotoxic at high dosages. 
     The Applicant previously found that a resistance inducer, salicylic acid, or functional derivatives thereof, synergizes with products having a direct fungicidal activity. In particular, some patents relates to inorganic copper compounds that effectively synergize with cupric salts of derivatives of salicylic acid, as described and claimed in international patent application WO03/043971. Basic copper salicylate, a cupric salt of salicylic acid, was previously indicated in patent FR572758 for use against fungal diseases. 
     As described in the subsequent patent application WO2005/094580, however, an important limitation in the use of cupric salts of derivatives of salicylic acid alone is linked to the risk that this use can cause undesired side-effects, which negatively influence the quality and quantity of the crop treated. 
     Furthermore, from a legislative point of view, the quantities of copper admissible in agriculture are becoming increasingly reduced. This is clearly linked to the high environmental impact of heavy metals, whose use is therefore becoming more and more limited. 
     Hydrated crystalline forms of copper monosalicylate and the relative preparation method have also been described in literature, such as, for example, in Wuhan University Journal of Natural Sciences, 2003, 425-427 and in Nippon Kagaku Kaishi, 1979, 4, 502-505. No biological activity correlated with the above-mentioned crystalline forms, however, has ever been mentioned. 
     The necessity is therefore particularly felt for finding alternative treatment capable of effectively limiting bacterioses, also at low dosages, which at the same time, can be well tolerated by agrarian crops. 
     The Applicant has now surprisingly found that copper salicylate, in certain crystalline forms, is capable of controlling bacterioses in plants, without causing undesired phytotoxicity effects. 
     An object of the present invention therefore relates to the use of at least one crystalline form of copper salicylate, with a molar ratio between copper and salicylic acid of (1:1), selected from crystalline form I, crystalline form II or crystalline form III and/or relative mixtures; wherein crystalline form I is characterized by an orthorhombic crystalline structure, a crystal unit cell indicated in Table 1 hereunder, a FTIR spectrum that has at least the absorption bands indicated in Table 2 hereunder, an XRD spectrum that has at least the characteristic peaks indicated in Table 3 hereunder and, preferably, a particle-size distribution ranging from 1.95 μm to 19.40 μm, preferably equal to about 6.17 μm; wherein crystalline form II is characterized by a triclinic crystalline structure, a crystal unit cell indicated in Table 1 hereunder, a FTIR spectrum that has at least the absorption bands indicated in Table 2 hereunder, an XRD spectrum that has at least the characteristic peaks indicated in Table 3 hereunder and, preferably, a particle-size distribution ranging from 15.37 μm to 100.30 μm, preferably equal to about 49.45 μm; wherein crystalline form III is characterized by a two-dimensional crystalline structure, a crystal unit cell indicated in Table 1 hereunder, a FTIR spectrum that has at least the absorption bands indicated in Table 2 hereunder, an XRD spectrum that has at least the characteristic peaks indicated in Table 3 hereunder 
     
       
         
           
               
               
               
               
               
               
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 Crystalline form 
                 a, Å 
                 b, Å 
                 c, Å 
                 α, ° 
                 β, ° 
                 γ, ° 
                 V, Å 3   
                 Z 
                 V/Z, Å 3   
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                 I 
                 18.868 
                 15.884 
                 5.042 
                 90 
                 90 
                 90 
                 1507 
                 8 
                 188 
               
               
                 II 
                 7.322 
                 10.677 
                 15.580 
                 70.67 
                 83.82 
                 76.31 
                 1116.09 
                 6 
                 186 
               
               
                 III 
                 24.46 
                 — 
                 5.66 
                 — 
                 90 
                 — 
                 — 
                 — 
                 — 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
               
                 Crystalline 
                 Crystalline 
                 Crystalline 
               
               
                 form I 
                 form II 
                 form III 
               
               
                   
               
             
            
               
                 3244.25 
                 3371.87 
                 3252.77 
               
               
                 2929.28 
                 3065.63 
                 3067.12 
               
               
                 1603.68 
                 1623.17 
                 2925.20 
               
               
                 1558.03 
                 1604.97 
                 1603.32 
               
               
                 1496.27 
                 1590.00 
                 1572.60 
               
               
                 1470.55 
                 1570.31 
                 1558.81 
               
               
                 1407.99 
                 1472.32 
                 1507.33 
               
               
                 1248.06 
                 1408.52 
                 1471.60 
               
               
                   
                 1253.54 
                 1258.08 
               
               
                   
               
            
           
         
       
     
                             TABLE 3               Crystalline   Crystalline   Crystalline       form I (2θ, °)   form II (2θ, °)   form III (2θ, °)                                            9.420   6.034   7.283       22.638   17.171   20.759       26.512   18.103   25.867       28.296   25.033   —       29.575   25.968   —       —   27.458   —                    
for the control of phytopathogenic bacteria.
 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a FTIR spectrum of the crystalline form I of copper salicylate. 
         FIG. 2  is a FTIR spectrum of the crystalline form II of copper salicylate. 
         FIG. 3  is a FTIR spectrum of the crystalline form III of copper salicylate. 
         FIG. 4  is a XRD spectrum of the crystalline form I of copper salicylate. 
         FIG. 5  is a XRD spectrum of the crystalline form II of copper salicylate. 
         FIG. 6  is a XRD spectrum of the crystalline form III of copper salicylate. 
     
    
    
     The crystalline form I of copper salicylate is characterized by a FTIR spectrum, having, as main absorption bands, the bands indicated in the above table, and substantially represented in  FIG. 1  (FTIR spectrum of the crystalline form I of copper salicylate). 
     Said crystalline form I of copper salicylate is also characterized by an XRD spectrum having, as characteristic peaks, the peaks indicated in the above table, and substantially represented in  FIG. 4  (XRD spectrum of the crystalline form I of copper salicylate). 
     Said crystalline form I of copper salicylate preferably has a very fine particle size. The particle-size distribution ranges from 1.95 μm to 19.40 μm, and is preferably equal to about 6.17 μm. 
     As can be observed from the FTIR spectrum in  FIG. 1 , the crystalline form I is characterized by the presence of [Ph(OH)CO 2 ] −  tridentate ions, bridge-coordinated with Cu(II) ions through the oxygen atoms of the carboxylate and undissociated phenolic oxygen. The small separation between the asymmetric stretching and symmetric stretching of the carboxyl bands indicates a bridge configuration of the carboxylate group between various Cu(II) centres. Furthermore, the copper ions carry a hydroxyl, interacting via hydrogen bonding with the surroundings. 
     The crystalline form II of copper salicylate is characterized by a FTIR spectrum, having, as main absorption bands, the bands indicated in the previous table, and substantially represented in  FIG. 2  (FTIR spectrum of the crystalline form II of copper salicylate). 
     Said crystalline form II of copper salicylate is also characterized by an XRD spectrum having, as characteristic peaks, the peaks indicated in the above table, and substantially represented in  FIG. 5  (XRD spectrum of the crystalline form II of copper salicylate). 
     Said crystalline form II of copper salicylate according to the present invention, preferably has a rougher particle size. The particle-size distribution ranges from 15.37 μm to 100.30 μm, and is preferably equal to about 49.45 μm. 
     As can be seen from the FTIR spectrum in  FIG. 2 , crystalline form II is characterized by [Ph(OH)CO 2 ] −  tridentate ions, coordinated through dissociated phenolic oxygen and with the monodentate, bidentate and/or chelated carboxylate group bridge-coordinated between multiple cupric sites. The presence of hydroxyl groups coordinated with Cu(II) ions and interacting through a hydrogen bond, can also be observed. 
     The crystalline form III of copper salicylate is characterized by a FTIR spectrum, having, as main absorption bands, the bands indicated in the previous table, and substantially represented in  FIG. 3  (FTIR spectrum of the crystalline form III of copper salicylate). 
     Said crystalline form III of copper salicylate is also characterized by an XRD spectrum having, as characteristic peaks, the peaks indicated in the above table, and substantially represented in  FIG. 6  (XRD spectrum of the crystalline form III of copper salicylate). 
     Preferred crystalline forms for the control of phytopathogenic bacteria are form I, form II and mixtures of crystalline form I and crystalline form II. 
     Said crystalline forms can be prepared according to methods well-known to skilled persons in the field, such as those indicated for example in WO2010/076038 for the above-mentioned crystalline form I, in Wuhan University Journal of Natural Sciences, 2003, 425-427 for the above-mentioned crystalline form II, or in Nippon Kagaku Kaishi, 1979, 4, 502-505 for the above-mentioned crystalline form III. 
     Copper salicylate, when used in one of the above-mentioned three crystalline forms I, II, III, or mixtures thereof, allows excellent levels of effectiveness to be obtained at particularly reduced doses of copper metal with respect to other commercial reference products such as copper hydroxide (Kocide® 3000). 
     As is evident from the results indicated in Example 9 below, on comparing the activity of copper salicylate in crystalline form I with the activity of commercial copper hydroxide, it can be observed that the crystalline form I according to the invention is much more active than the commercial product on bacteriosis of tomato plants. Furthermore, the above crystalline form I is also more persistent with intervals of 10 days between one treatment of the crop and the subsequent treatment, with respect to copper hydroxide with intervals of 7 days between one treatment and another. 
     As already specified, the crystalline forms I, II and III of copper salicylate have a high bactericidal activity and do not show any phytotoxicity with respect to the crops of application. These characteristics make them particularly suitable for use in the agrarian field in defense against bacteria. 
     Examples of bacteria that can be effectively reduced with the crystalline forms I, II and III of copper salicylate are  Pseudomonas syringae  pv tomato of tomato plants,  Pseudomonas syringae  pv actinidiae of kiwi plants,  Pseudomonas lachrymans  of cucurbits,  Xanthomonas campestris  pv campestris of lettuces,  Pseudomonas viridiflava  of vegetables,  Xanthomonas juglandis  of walnut trees,  Xylella fastidiosa  of olive trees, etc. 
     The three crystalline forms of copper salicylate according to the present invention can also be validly used in the control of phytopathogenic fungi. Examples of fungi that can be effectively controlled with the crystalline forms I, II and III of copper salicylate are  Plasmopara viticola  of vines,  Phytopthora infestans  of tomato and potato plants,  Uromyces appendiculatus  on beans,  Erysiphe cichoracearum  on cucumbers,  Fusicoccum amygdale  of peach trees,  Cercospora beticola  of sugar beet,  Uncinula necator  of vines,  Bremia lactucae  of lettuces, etc. 
     For practical uses in agriculture, it is often preferable to use copper salicylate suitably formulated in agronomic compositions comprising one or more crystalline forms of the present invention and agronomically acceptable coformulants. 
     As previously indicated, the present invention also relates to the use of bactericidal agronomic compositions comprising one or more crystalline forms I, II and III of copper salicylate, a solvent and/or diluent, possibly one or more surfactants and other agronomically acceptable coformulants. 
     Compositions can be used in the form of dry powders, wettable powders, emulsifiable concentrates, microemulsions, pastes, granulates, solutions, suspensions, etc.: the choice of the type of composition depends on the specific use. 
     The compositions are prepared according to known methods, for example by diluting or dissolving the active substance with a solvent medium and/or solid diluent, possibly in the presence of surfactants. 
     Kaolin, alumina, silica, talc, bentonite, gypsum, quartz, dolomite, attapulgite, montmorillonite, diatomaceous earth, cellulose, starch, etc., can be used as inert solid diluents, or carriers. 
     Inert liquid diluents that can be used are water, or organic solvents such as aromatic hydrocarbons (xylols, blends of alkyl benzenes, etc.), aliphatic hydrocarbons (hexane, cyclohexane, etc.) halogenated aromatic hydrocarbons (chlorobenzene, etc.), alcohols (methanol, propanol, butanol, octanol, etc.), esters (isobutyl acetate, etc.), ketones (acetone, cyclohexanone, acetophenone, isophorone, ethylamylketone etc.), or vegetable or mineral oils or mixtures thereof, etc. 
     Propellant gases such as butane, propane, halogenated hydrocarbons, nitrogen or carbon dioxide can be used as liquefied diluents or liquefied substances that gasify at room temperature and atmospheric pressure. 
     Surfactants that can be used are wetting and emulsifying agents of the non-ionic type (polyethoxylated alkyl phenols, polyethoxylated fatty alcohols, etc.), of the anionic type (alkylbenzenesulfonates, alkylsulfonates, etc.), of the cationic type (alkyl ammonium quaternary salts, etc.). 
     Dispersing agents can also be added (for example lignin and its salts, cellulose derivatives, alginates, etc.), stabilizers (for example antioxidants, UV absorbers, etc.). 
     The concentration of copper salicylate in the above compositions can vary within a wide range and depends on various factors. It varies in relation to the crystalline form of copper salicylate, the applications for which said compositions are destined, the environmental conditions and the type of formulation adopted. The concentration of copper salicylate generally ranges from 0.1 to 90% by weight with respect to the total weight of the composition, preferably from 0.5 to 90% by weight. 
     The crystalline forms of the present invention as such or formulated can be used in a mixture with other active principles such as, for example, insecticides, acaricides, nematocides, herbicides, fungicides, bactericides different from copper salicyl ate, fertilizers, biostimulants, etc. for broadening their spectrum or preventing resistance. 
     Said agronomic compositions can comprise a crystalline form I, II and III of copper salicylate and/or mixtures thereof and at least a second active principle selected from insecticides, acaricides, nematocides, herbicides, fungicides, bactericides different from copper salicylate, fertilizers, biostimulants. 
     In some cases, the mixtures thus obtained have a synergistic effect between the components, which causes the mixture, for example, to exert a higher activity with respect to that of the single elements of which it is composed. 
     Examples of insecticides, acaricides, nematocides that can be added to the compositions containing at least one crystalline form of copper salicylate are the following: abamectin, acetamiprid, acrinathrin, alphacypermethrin, alphamethrin, azadirachtin,  Bacillus subtilis, Bacillus thuringiensis , Beauveria bassiana, betacyfluthrin, bifenazate, bifenthrin, buprofezin, chlorpyrifos, chlorpyrifos M, clofentezine, cyhalothrin, cyhexatin, cypermethrin, cyromazine, chloropicrin, clorantranilipide, clotianidin, deltamethrin, diflubenzuron, dimethoat, dazonet, sulfuryl difluoride, dimethyldisulfide, emamectin, esfenvalerate, ethoprophos, etofenprox, etoxazole, fenamiphos, fenazaquin, fenoxycarb, fenpyroximate, fipronil, fluazinam, flufenoxuron, fluvalinate, fosthiazate, formentanate, flonicamid, formet, viruses, hexythiazox, imidaclopridi, indoxacarb, lambda-cyhalothrin, lufenuron malathion, metaldehyde, methamidophos, Metharhizium spp, methiocarb, methomyl, methoxyfenozide, milbemectin, metaflumizone, metam sodium, metam potassium, oxamyl, Paecilomyces fumosoroseus, phosmet, pirimicarb, pirimiphos M, pyrethrum, pyridaben, pyriproxyfen, piperonyl butoxide, spinosad, spironesifen, spirotetramat, spinetoran, spirodiclofen, tau-fluvalinate, tebufenozide, tebufenpyrad, teflubenzuron, tefluthrin, thiacloprid, triflumuron, zeta-cypermethrin, (1R-cis)-[5-(phenylmethyl)-3-furanyl]-methyl-3-[(dihydro-2-oxo-3(2H)-furanylidene) methyl]-2,2-dimethyl-cyclopropane-carboxylate, (3-phenoxyphenyl)-methyl-2,2,3,3-tetra-methylcyclopropanecarboxylate, 1-[(2-chloro-5-thiazolyl)methyl]-5-triazine 2-(1H)-imine, 2-(2-chloro-6-fluorophenyl)-4-[4-(1,1-dimethylethyl)-phenyl]-4,5-dihydro-oxazol, 2-(acetyloxy)-3-dodecyl-1,4-naph-thalenedione, 2-chloro-N-[[[4-(1-phenylethoxy)-phenyl]-amino]-carbonyl]-benzamide, 2-chloro-N-[[[4-(2,2-dichloro-1,1-difluoroethoxy)-phenyl]-amino]-carbonyl]-benzamide, 3-methylphenyl-propylcarbamate, 4-[4-(4-ethoxyphenyl)-4-methylpentyl]-1-fluoro-2-phenoxy-benzene, 4-chloro-2-(1,1-dimethylethyl)-5-[[2-(2,6-dimethyl-4-phenoxyphenoxy)ethyl]thio]-3-(2H)-pyrid-azinone, 4-chloro-2-(2-chloro-2-methylpropyl)-5-[(6-iodo-3-pyridinyl)(2-chloro-2-methylpropyl)-5-[(6-iodo-3-pyridinyl)-methoxy]-3-(2H)pyridazinone, 4-chloro-5-[(6-chloro-3-pyridinyl)methoxy]-2-(3,4-dichloro-phenyl)-3(2H)pyridazinone,  Bacillus thuringiensis  strain EG-2348, [2-benzoyl-1-(1,1-dimethyl ethyl)-hydrazine] benzoic acid, 2,2-dimethyl-3-(2,4-dichlorophenyl)-2-oxo-1-oxaspiro [4.5]-dec-3-en-4-yl butanoate, [3-[(6-chloro-3-pyridinyl)-methyl]-2-thiazolidinylidene]-cyanamide, dihydro-2-(nitro-methylene)-2H-1,3-thiazine-3(4H)-carboxaldehyde, ethyl[2-[[1,6-dihydro-6-oxo-1-(phenylmethyl)-4-pyrid-azinyl]oxy]ethyl]-carbamate, N-(3,4,4-trifluoro-1-oxo-3-butenyl)-glycine, N-(4-chlorophenyl)-3-[4-(difluoro-methoxy)-phenyl]-4,5-dihydro-4-phenyl-1H-pyrazole- 1 -carboxamide, N-[(2-chloro-5-thiazolyl)-methyl]-N′-methyl-N″-nitro-guanidine, N-methyl-N′-(1-methyl-2-propenyl)-1,2-hydrazinedicarbothioamide, N-methyl-N′-2-propenyl-1,2-hydrazinedicarbothioamide, O,O-diethyl[2-(dipropylamino)-2-oxoethyl]-ethyl-phosphoroamido-thioate. 
     Examples of herbicides that can be added to the compositions containing at least one crystalline form I, II or III of copper salicylate are the following: acetochlor, acifluorfen, aclonifen, AKH-7088 ({methyl (E,Z)-[[[1-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrophenyl]-2-methoxyethylidene]amino]acetate}), alachlor, alloxydim, ametryn, amicarbazone, amidosulfuron, amitrole, anilofos, asulam, atrazine, azafenidin, azimsulfuron, aziprotryne, BAY MKH 6561 (methyl 2-({[(4-methyl-5-oxo-3-propoxy-4,5-dihydro-1H-1,2,4-triazol-1-yl)carbonyl] amino}sulfonyl)benzoate sodium salt), beflubutamid, benazolin, benfluralin, benfuresate, bensulfuron, bensulide, bentazone, benzfendizone, benzobicyclon, benzofenap, benzthiazuron, bifenox, bilanafos, bispyribac-sodium, bromacil, bromobutide, bromofenoxim, bromoxynil, butachlor, butafenacil, butamifos, butenachlor, butralin, butroxydim, butylate, cafenstrole, carbetamide, carfentrazone-ethyl, chlomethoxyfen, chloramben, chlorbromuron, chlorbufam, chlorflurenol, chloridazon, chlorimuron, chlornitrofen, chlorotoluron, chloroxuron, chlorpropham, chlorsulfuron, chlorthal, chlorthiamid, cinidon ethyl, cinmethylin, cinosulfuron, clethodim, clodinafop, clomazone, clomeprop, clopyralid, cloransulam-methyl, cumyluron (JC-940), cyanazine, cycloate, cyclosulfamuron, cycloxydim, cyhalofop-butyl, 2,4-D, 2,4-DB, daimuron, dalapon, desmedipham, desmetryn, dicamba, dichlobenil, dichlorprop, dichlorprop-P, diclofop, diclosulam, diethatyl, difenoxuron, difenzoquat, diflufenican, diflufenzopyr, dimefuron, dimepiperate, dimethachlor, dimethametryn, dimethenamid, dinitramine, dinoseb, dinoseb acetate, dinoterb, diphenamid, dipropetryn, diquat, dithiopyr, 1-diuron, eglinazine, endothal, EPTC, esprocarb, ethalfluralin, ethametsulfuron-methyl, ethidimuron, ethiozin (SMY 1500), ethofumesate, ethoxyfen-ethyl (HC-252), ethoxysulfuron, etobenzanid (HW 52), fenoxaprop, fenoxaprop-P, fentrazamide, fenuron, flamprop, flamprop-M, flazasulfuron, florasulam, fluazifop, fluazifop-P, fluazolate (JV 485), flucarbazone-sodium, fluchloralin, flufenacet, flufenpyr ethyl, flumetsulam, flumiclorac-pentyl, flumioxazin, flumipropin, fluometuron, fluoroglycofen, fluoronitrofen, flupoxam, flupropanate, flupyrsulfuron, flurenol, fluridone, flurochloridone, fluroxypyr, flurtamone, fluthiacet-methyl, fomesafen, foramsulfuron, fosamine, furyloxyfen, glufosinate, glyphosate, halosulfuron-methyl, haloxyfop, haloxyfop-P-methyl, hexazinone, imazamethabenz, imazamox, imazapic, imazapyr, imazaquin, imazethapyr, imazosulfuron, indanofan, iodosulfuron, ioxynil, isopropalin, isoproturon, isouron, isoxaben, isoxachlortole, isoxaflutole, isoxapyrifop, KPP-421, lactofen, lenacil, linuron, LS830556 [[[2-methyl(methylsulfonyl)amino]-2-oxoethyl]amino]-methylphosphonic acid, MCPA 2-methyl-4-chlorophenoxyacetic acid, MCPA-thioethyl, MCPB (4-(4-chloro-2-methylphenoxy)butanoic acid), mecoprop, mecoprop-P, mefenacet, mesosulfuron, mesotrione, metamitron, metazachlor, methabenzthiazuron, methazole, methoprotryne, methyldymron, metobenzuron, metobromuron, metolachlor, S-metolachlor, metosulam, metoxuron, metribuzin, metsulfuron, molinate, monalide, monolinuron, naproanilide, napropamide, naptalam, NC-330 (methyl 5-[(4,6-dimethylpyrimidin-2-yl)carbamoyl-sulfamoyl]1-pyridin-2-yl pyrazol-4-carboxylate), neburon, nicosulfuron, nipyraclofen, norflurazon, orbencarb, oryzalin, oxadiargyl, oxadiazon, oxasulfuron, oxaziclomefone, oxyfluorfen, paraquat, pebulate, pendimethalin, penoxsulam, pentanochlor, pentoxazone, pethoxamid, phenmedipham, picloram, picolinafen, piperophos, pretilachlor, primisulfuron, prodiamine, profluazol, proglinazine, prometon, prometryne, propachlor, propanil, propaquizafop, propazine, propham, propisochlor, propyzamide, prosulfocarb, prosulfuron, pyraclonil, pyraflufen-ethyl, pyrazogyl (HSA-961), pyrazolynate, pyrazosulfuron, pyrazoxyfen, pyribenzoxim, pyributicarb, pyridafol, pyridate, pyriftalid, pyriminobac-methyl, pyrithiobac-sodium, pyroxasulfone quinclorac, quinmerac, quizalofop, quizalofop-P, rimsulfuron, sethoxydim, siduron, simazine, simetryn, sulcotrione, sulfentrazone, sulfometuron-methyl, sulfosulfuron, 2,3,6-TBA, TCA-sodium, tebutam, tebuthiuron, tepraloxydim, terbacil, terbumeton, terbuthyl-azine, terbutryn, thenylchlor, thiazafluron, thiazopyr, thidiazimin, thifensulfuron-methyl, thiobencarb, tiocarbazil, tioclorim, tralkoxydim, tri-allate, triasulfuron, triaziflam, tribenuron, triclopyr, trietazine, trifloxysulfuron, trifluralin, triflusulfuron-methyl, tritosulfuron, UBI-C4874 (quizalofop-P), vernolate. 
     Examples of fungicides that can be added to the compositions containing at least one crystalline form I, II or III of copper salicylate are the following: acibenzolar, ametoctradin, amisulbrom, ampropylfos, anilazine, azaconazole, azoxystrobin, benalaxyl, benalaxyl-M, benomyl, benthiavalicarb, bitertanol, bixafen, blasticidin-S, boscalid, bromuconazole, bupirimate, buthiobate, captafol, captan, carbendazim, carboxin, carpropamid, chinomethionat, chloroneb, chlorothalonil, chlozolinate, cyazofamid, cyflufenamid, cymoxanil, cyproconazole, cyprodinil, debacarb, dichlofluanid, dichlone, diclobutrazol, diclomezine, dicloran, diclocymet, diethofencarb, difenoconazole, diflumetorim, dimethirimol, dimethomorph, dimoxystrobin, diniconazole, dinocap, dipyrithione, ditalimfos, dithianon, dodemorph, dodine, edifenphos, epoxiconazole, etaconazole, ethab oxam, ethirimol, ethoxyquin, etridiazole, famoxadone, fenamidone, fenaminosulf, fenapanil, fenarimol, fenbuconazole, fenfuram, fenhexamid, fenoxanil, fenpiclonil, fenpropidin, fenpropimorph, fenpyrazamine, fentin, ferbam, ferimzone, fluazinam, fludioxonil, fluindapyr ((RS)-3-(difluoromethyl)-N-(7-fluoro-2,3-dihydro-1,1,3-trimethyl-1H-inden-4-yl)-1-methyl-1H-pyrazole-4-carboxamide), flumetover, flumorph, fluopicolide, fluopyram, fluoroimide, fluotrimazole, fluoxastrobin, fluquinconazole, flusilazole, flusulfamide, flutianil, flutolanil, flutriafol, fluxapyroxad, folpet, fosetyl-aluminium, fuberidazole, furalaxyl, furametpyr, furconazole, furconazole-cis, guazatine, hexaconazole, hymexazol, hydroxyquinoline sulfate, imazalil, imibenconazole, iminoctadine, ipconazole, iprobenfos, iprodione, isoprothiolane, iprovalicarb, isopyrazam, isotianil, kasugamycin, kresoxim-methyl, mancopper, mancozeb, mandipropamid, maneb, mebenil, mepanipyrim, mepronil, meptyldinocap, metalaxyl, metalaxyl-M, metconazole, methfuroxam, metiram, metominostrobin, metrafenone, metsulfovax, myclobutanil, natamycin, nicobifen, nitrothal-isopropyl, nuarimol, ofurace, orysastrobin, oxadixyl, oxpoconazole, oxycarboxin, pefurazoate, penconazole, pencycuron, penflufen, pentachlorofenol and its salts, penthiopyrad, phthalide, picoxystrobin, piperalin, Bordeaux mixture, polyoxins, probenazole, prochloraz, procymidone, propamocarb, propiconazole, propineb, proquinazid, prothiocarb, prothioconazole, pyracarbolid, pyraclostrobin, pyrametostrobin, pyraoxystrobin, pyrazophos, pyribencarb, pyrifenox, pyrimethanil, pyriofenone, pyroquilon, pyroxyfur, quinacetol, quinazamid, quinconazole, quinoxyfen, quintozene, rabenzazole, copper hydroxide, copper oxychloride, copper (I) oxide, copper sulfate, sedaxane, silthiofam, simeconazole, spiroxamine, streptomycin, tebuconazole, tebufloquin, tetraconazole, thiabendazole, thiadifluor, thicyofen, thifluzamide, thiophanate, thiophanate-methyl, thiram, tiadinil, tioxymid, tolclofos-methyl, tolylfluanid, triadimefon, triadimenol, triarimol, triazbutil, triazoxide, tricyclazole, tridemorf, trifloxystrobin, triflumizole, triforine, triticonazole, uniconazole, uniconazole-P, validamycin, valifenalate, vinclozolin, zineb, ziram, sulfur, zoxamide. 
     Examples of bactericides that can be added to the compositions containing at least one crystalline form I, II or III of copper salicylate are the following: bronopol, dichlorophen, nitrapyrina, nickel dimethyldithi ocarbamate, kasugamycin, octhilinone, furancarboxylic acid, probenazole, streptomycin, tecloftalam, copper hydroxide, copper oxychloride, copper (I) oxide, copper sulfate. 
     Examples of fertilizers and biostimulants that can be added to the compositions containing at least one crystalline form I, II or III of copper salicylate are the following: mixtures of amino acids and/or oligopeptides of an animal and/or vegetable origin, 4-thiazolidinecarboxylic acid, 4-acetylthiazolidine-carboxylic acid, ectoin, phytosterols. 
     The above compositions are capable of exerting a bactericidal action that can be of a curative or preventive nature and generally have an extremely low or zero phytotoxicity with respect to the crops treated. 
     When the compositions comprise a crystalline form I, II or III of copper salicylate and at least a further known active principle, the weight ratios in the above compositions, between copper salicylate and the further known active principles, range according to the compounds selected and can normally be within the range of 1:100 to 100:1, preferably from 1:10 to 10:1. 
     The total concentration of active components in the above compositions can vary within a wide range; they generally range from 1% to 99% by weight with respect to the total weight of the composition, preferably from 5 to 90% by weight with respect to the total weight of the composition. 
     The crystalline forms I, II and/or III of copper salicylate or the compositions containing them can be applied to the crop via the leaves, or to the soil by means of fertigation, or incorporation into the ground, or through seed care. 
     A further object of the present invention therefore relates to a method for the control of bacteria in cultivated areas, which consists in applying effective and non-phytotoxic doses of compositions comprising at least one crystalline form I, II or III of copper salicylate and, optionally, one or more known active principles compatible therewith, on any part of the plant to be protected. 
     The quantity of compound to be applied for obtaining the desired effect can vary in relation to different factors such as, for example, the compound used, the crop to be protected, the degree of infestation, the climatic conditions, the characteristics of the soil, the method of application, etc. 
     Doses of copper salicylate ranging from 100 g to 2,000 g per hectare of agricultural crop or, in the case of compositions comprising other known active principles, overall doses of active principles ranging from 100 g to 2,000 g per hectare of agricultural crop, generally provide a sufficient control. 
     Doses of copper salicylate ranging from 100 g to 600 g per hectare of agricultural crop are preferably used. 
     The following illustrative and non-limiting examples are provided for a better understanding of the invention. 
     EXPERIMENTAL PART 
     Example 1 
     Preparation of the Crystalline Form I of Copper Salicylate 
     A solution of NH 4 OH at 28%, 181 g (1.45 moles) in 300 ml was added to a suspension of salicylic acid, 100 g (0.724 moles) in 400 ml of water, until the complete solubilization had been obtained. An aqueous solution of CuCl 2 , 97.3 g (0.724 moles) in 300 ml was then added and the system was left under stirring at room temperature for 8 hours. During the reaction time, the precipitation of a fine green solid first took place, whose colour then turned to ochre, accompanied by an increase in the viscosity and rise in the pH to a final value of about 6, requiring an adequate mixing system. The reaction mixture was ground and then filtered on a Buchner funnel. It was then washed with a large quantity of water to eliminate the ammonium chloride formed, and dried in air obtaining 150 g of the desired product. 
     The FT-IR spectrum indicated in  FIG. 1 , was obtained from this product. The spectrum was recorded within the range of 4,000-400 cm −1  in reflectance with an AVATAR 370 FT-IT Thermo-Nicolet instrument, effecting 64 scans. The sample was dispersed in KBr. 
     The XRD spectrum indicated in  FIG. 4 , was obtained from this product. The following experimental conditions were used for the analysis: 
     Bruker AXS D8 Advance diffractometer; setting of the generator 40 kV, 40 mA; divergence slit=0.3°; Goniometric radius=300 mm; radiation Cu-Kα, λ=1.5418 Å; position sensitive detector Lynx-eye; nickel filter on the diffracted beam. Geometry θ:θ; step scan with Δ2θ=0.02°. Silicon sample-holder (monocrystal) 200μ deep. Peak search and profile fitting with Fundamental Parameters Approach (Cheary &amp; Coelho, 1992), with the program TOPAS-R (v. 3.0, Bruker). 
     The product of the present example has a particle-size distribution equal to about 6.17 μm for D 50 , 1.95 μm for D 10  and 19.40 μm for D 90 . 
     The term “D 50 ” represents the value of the diameter of the sieve which allows the passage of 50% of the particles. 
     The term “D 10 ” represents the value of the diameter of the sieve which allows the passage of 10% of the particles. 
     The term “D 90 ” represents the value of the diameter of the sieve which allows the passage of 90% of the particles. 
     The particle-size distribution was measured with a MALVERN MASTERSIZER instrument according to standard methods. 
     Example 2 
     Preparation of the Crystalline Form I of Copper Salicylate 
     A solution of NaOH, 58 g (1.45 moles) in 300 ml was added to a suspension of salicylic acid, 100 g (0.724 moles) in 400 ml of water, until the complete solubilization had been obtained. An aqueous solution of CuSO 4 , 180.8 g (0.724 moles) in 300 ml was then added and the system was left under stirring at room temperature for 8 hours. During the reaction time, the precipitation of a fine green solid first took place, whose colour then turned to ochre, accompanied by an increase in the viscosity and rise in the pH to a final value of about 6, requiring an adequate mixing system. The reaction mixture was ground and then filtered on a Buchner funnel. It was then washed with a large quantity of water to eliminate the ammonium chloride formed, and dried in air obtaining 150 g of the desired product. 
     Example 3 
     Preparation of the Crystalline Form II of Copper Salicylate 
     70.6 g (0.724 moles) of Cu(OH) 2  were added, in portions, to a suspension of 100 g (0.724 moles) of salicylic acid in 1 l of water, maintained at 60-65° C. to increase its solubility, and the system was left under stirring at this temperature for 2 hours. During the reaction time, the precipitation of a dark green solid took place, accompanied by an increase in the pH to a final value of about 6. The reaction mixture thus obtained was filtered on a Buchner funnel and dried in air. Alternatively, the mixture can be used as slurry ready for the subsequent formulation. 
     The FT-IR spectrum indicated in  FIG. 2 , was obtained from this product. The spectrum was recorded within the range of 4,000-400 cm −1  in reflectance with an AVATAR 370 FT-IT Thermo-Nicolet instrument, effecting 64 scans. The sample was dispersed in KBr. 
     The XRD spectrum indicated in  FIG. 5 , was obtained from this product. The following experimental conditions were used for the analysis: 
     Bruker AXS D8 Advance diffractometer; setting of the generator 40 kV, 40 mA; divergence slit =0.3°; Goniometric radius=300 mm; radiation Cu-Kα, λ=1.5418 Å; position sensitive detector Lynx-eye; nickel filter on the diffracted beam. Geometry θ:θ; step scan with Δ2θ=0.02°. Silicon sample-holder (monocrystal) 200μ deep. Peak search and profile fitting with Fundamental Parameters Approach (Cheary &amp; Coelho, 1992), with the program TOPAS-R (v. 3.0, Bruker). 
     The product obtained in the present example has a particle-size distribution equal to about 49.45 μm for D 50 , 15.37 μm for D 10  and 100.30 μm for D 90 . 
     The particle-size distribution was measured with a MALVERN MASTERSIZER instrument according to standard methods. 
     Example 4 
     Preparation of the Crystalline Form III of Copper Salicylate 
     The solid obtained according to Example 1 or according to Example 2 was subjected to thermal treatment using an electric oven at a temperature equal to about 90° C. until a homogeneous colour and constant weight had been reached. A solid having a brick-red colour was obtained. 
     The FT-IR spectrum indicated in  FIG. 3 , was obtained from this product. 
     The spectrum was recorded within the range of 4000-400 cm −1  in reflectance with an AVATAR 370 FT-IT Thermo-Nicolet instrument, effecting 64 scans. The sample was dispersed in KBr. 
     The XRD spectrum indicated in  FIG. 6 , was obtained from this product. The following experimental conditions were used for the analysis: 
     Bruker AXS D8 Advance diffractometer; setting of the generator 40 kV, 40 mA; divergence slit=0.3°; Goniometric radius=300 mm; radiation Cu-Kα, λ=1.5418 Å; position sensitive detector Lynx-eye; nickel filter on the diffracted beam. Geometry θ:θ; step scan with Δ2θ=0.02°. Silicon sample-holder (monocrystal) 200μ deep. Peak search and profile fitting with Fundamental Parameters Approach (Cheary &amp; Coelho, 1992), with the program TOPAS-R (v. 3.0, Bruker). 
     Example 5 
     Preparation of Copper Salicylate in the Form of a Wettable Powder (WP). 
     A formulation in the form of a wettable powder at 50% by weight (WP) was prepared by mixing and suitably grinding the following ingredients: 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                   
                 QUANTITY % 
               
               
                   
                 INGREDIENTS 
                 (weight/weight) 
               
               
                   
                   
               
             
            
               
                   
                 Technical copper salicylate at 99% 
                 50.5  
               
               
                   
                 Crystalline form I Example 1 
                 (equal to 14.6% of Cu) 
               
               
                   
                 (Cu content = 29%) 
               
               
                   
                 Sodium alkyl naphthalene sulfonate 
                 1.5 
               
               
                   
                 Sodium polycarboxylate 
                 1.0 
               
               
                   
                 Sodium ligninsulfonate 
                 3.0 
               
               
                   
                 Silica 
                 1.0 
               
               
                   
                 Calcium carbonate 
                 complement to 100 
               
               
                   
                   
               
            
           
         
       
     
     Example 6 
     Another formulation was prepared as a wettable powder (WP), analogously to Example 5: 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                   
                 QUANTITY % 
               
               
                   
                 INGREDIENTS 
                 (weight/weight) 
               
               
                   
                   
               
             
            
               
                   
                 Technical copper salicylate at 99% 
                 50.5  
               
               
                   
                 Crystalline form I Example 1 
                 (equal to 14.6% of Cu) 
               
               
                   
                 (Cu content = 29%) 
               
               
                   
                 Sodium alkyl naphthalene sulfonate 
                 1.0 
               
               
                   
                 Sodium polycarboxylate 
                 2.0 
               
               
                   
                 Calcium ligninsulfonate 
                 5.0 
               
               
                   
                 Silica 
                 1.0 
               
               
                   
                 Kaolin 
                 complement to 100 
               
               
                   
                   
               
            
           
         
       
     
     Example 7 
     Preparation of Copper Salicylate in the Form of Dispersible Granules (WG). 
     A formulation in the form of dispersible granules at 20% of copper (WG) was prepared by adding the following ingredients to the slurry obtained in Example 1: 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                   
                 QUANTITY % 
               
               
                   
                 INGREDIENTS 
                 (weight/weight) 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Sodium alkyl naphthalene sulfonate 
                 2.3 
               
               
                   
                 Sodium polynaphthalene sulfonate 
                 9.1 
               
               
                   
                 Calcium ligninsulfonate 
                 11.4 
               
               
                   
                 Kaolin 
                 48 
               
               
                   
                   
               
            
           
         
       
     
     The slurry thus prepared was granulated by evaporation of the water present (fluid-bed granulation), obtained the final WG with the following composition: 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                   
                 QUANTITY % 
               
               
                   
                 INGREDIENTS 
                 (weight/weight) 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Technical copper salicylate at 99% 
                 69 
               
               
                   
                 Crystalline form I Example 2 
                 (equal to 20% of Cu) 
               
               
                   
                 (Cu content = 29%) 
               
               
                   
                 Sodium alkyl naphthalene sulfonate 
                 1.0 
               
               
                   
                 Sodium polycarboxylate 
                 4.0 
               
               
                   
                 Calcium ligninsulfonate 
                 5.0 
               
               
                   
                 Kaolin 
                 complement to 100 
               
               
                   
                   
               
            
           
         
       
     
     Example 8 
     Preparation of Copper Salicylate in the Form of a Concentrated Aqueous Suspension (SC). 
     A formulation in the form of a concentration aqueous suspension at 5% of copper (SC) was prepared by adding the following ingredients to the slurry obtained in Example 1: 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                   
                 QUANTITY % 
               
               
                   
                 INGREDIENTS 
                 (weight/weight) 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Technical copper salicylate at 99% 
                 17.4 
               
               
                   
                 Crystalline form I Example 2 
                 (equal to 5% of Cu) 
               
               
                   
                 (Cu content = 29%) 
               
               
                   
                 Sodium polynaphthalenemethane sulfonate 
                 4 
               
               
                   
                 Calcium ligninsulfonate 
                 6 
               
               
                   
                 Monopropylene glycol 
                 7 
               
               
                   
                 Defoaming agent 
                 0.5 
               
               
                   
                 Antibacterial agent 
                 0.25 
               
               
                   
                 Xanthan gum 
                 5 
               
               
                   
                 Water 
                 complement to 100 
               
               
                   
                   
               
            
           
         
       
     
     Example 9 
     Biological Evaluation of the Crystalline Form I of Copper Salicylate on  Pseudomonas syringae  pv tomato 
     Tomato plants at the 4-leaf stage were transplanted in plots in a cold greenhouse, replicated four times and treated according to the protocol indicated in the following table with knapsack pumps, with a volume of 500 l per hectare. 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                   
                 Conc. form 
                   
                   
                 Quantity 
                 Quantity 
               
               
                 Treatment 
                 (%) 
                 Unit 
                 Type 
                 g a.i.*/1001 
                 g p.f.**/1001 
               
               
                   
               
             
            
               
                 Control 
                 — 
                 — 
                 — 
                 — 
                 — 
               
               
                 Crystalline 
                 5 
                 % W/W 
                 SC 
                 25 
                 500 
               
               
                 form I 
                   
                   
                   
                 12.5 
                 250 
               
               
                 Example 1 
                   
                   
                   
                 6.25 
                 125 
               
               
                   
                   
                   
                   
                 3.125 
                 62.5 
               
               
                 Cu(OH) 2   
                 40 
                 % W/W 
                 WG 
                 25 
                 62.5 
               
               
                   
                   
                   
                   
                 12.5 
                 31.25 
               
               
                   
                   
                   
                   
                 6.25 
                 15.625 
               
               
                 Salicylic 
                 100 
                 % W/W 
                 TQ 
                 54.2 
                 54.2 
               
               
                 acid 
                   
                   
                   
                 27.2 
                 27.2 
               
               
                   
                   
                   
                   
                 13.6 
                 13.6 
               
               
                   
               
               
                 *a.i. = active ingredient 
               
               
                 **p.f. = finished product 
               
            
           
         
       
     
     In the above table, SC is an abbreviation of “concentrated aqueous suspension” and TQ of “as such”. 
     The day after application, the plants were infected with a suspension of bacterial cells previously cultivated in Nutrient Broth culture broth, at a bacterium-broth concentration of 1:4. 
     The inoculum, kept under stirring for 2 days, was sprayed on the plants in the morning, in guttation phase, with knapsack sprayers. 
     Visual surveys were effected 7 days after each infection/treatment, analyzing 50 plants per plot, analyzing the percentage of plants infected and the percentage of leaf surface affected. 
     The results obtained are indicated in the following table: 
     
       
         
           
               
               
               
               
               
             
               
                   
                   
               
               
                   
                   
                 Quantity 
                 Quantity infected 
                 Leaf surface 
               
               
                   
                 Treatment 
                 g a.i./1001 
                 plants (%) 
                 affected (%) 
               
               
                   
                   
               
             
            
               
                   
                 Control 
                 — 
                 50.7 
                 6.4 
               
               
                   
                 Crystalline 
                 25 
                 26.3 
                 2.3 
               
               
                   
                 form I 
                 12.5 
                 26.0 
                 2.7 
               
               
                   
                 Example 1 
                 6.25 
                 12.5 
                 1.7 
               
               
                   
                   
                 3.125 
                 26.6 
                 2.0 
               
               
                   
                 Cu(OH) 2   
                 25 
                 31.0 
                 3.9 
               
               
                   
                   
                 12.5 
                 35.7 
                 4.9 
               
               
                   
                   
                 6.25 
                 37.5 
                 3.4 
               
               
                   
                 Salicylic 
                 54.2 
                 36.3 
                 3.5 
               
               
                   
                 acid 
                 27.2 
                 31.5 
                 3.9 
               
               
                   
                   
                 13.6 
                 40.7 
                 4.7 
               
               
                   
                   
               
            
           
         
       
     
     It can be noted that the bactericidal activity of the crystalline form I of copper salicylate of Example 1 is much higher with respect to the activity of the single components of copper salicylate, i.e. salicylic acid and copper hydroxide. 
     Furthermore, the percentage of plants infected after the treatment with the crystalline form I of Example 1 at 6.25 g a.i./100 l is about a third (12.5%) with respect to that after treatment with copper hydroxide (37.5%) with the same quantity of copper metal. 
     Finally, it should be pointed out that no symptom of phytotoxicity was observed in the four tests carried out with the crystalline form I of Example 1, unlike the single components that proved to be phytotoxic at high doses. 
     Example 10 (Comparative) 
     Biological Evaluation of Cupric Salts of Carboxylic Acids on  Pseudomonas syringae  pv tomato 
     A comparison was effected between the bactericidal activity of various cupric salts of carboxylic acids described in patent application US2009/136581 with that of crystalline foiin 1. 
     Tomato plants at the 4-leaf stage were transplanted in plots in a cold greenhouse, replicated four times and treated with the various compounds being tested by means of knapsack pumps, with a volume of 500 l per hectare. 
     The day after application, the plants were infected with a suspension of bacterial cells previously cultivated in Nutrient Broth culture broth, at a bacterium-broth concentration of 1:4. 
     The inoculum, kept under stirring for 2 days, was sprayed on the plants in the morning, in guttation phase, with knapsack sprayers. 
     Visual surveys were effected 7 days after each infection/treatment, analyzing 10 plants per plot, analyzing the percentage of leaf surface affected. 
     The results obtained are indicated in the following table: 
     
       
         
           
               
               
               
               
             
               
                   
               
               
                   
                 Dosage 
                 Leaf surface 
                 Phytotoxicity 
               
               
                 Treatment 
                 (ppm a.i.) 
                 affected (%) 
                 (%) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 Control 
                   
                 50 
                 0 
               
               
                 Crystalline form I 
                 250 
                 5 
                 0 
               
               
                 Example 1 
               
               
                 Crystalline form II 
                 250 
                 5 
                 0 
               
               
                 Example 3 
               
               
                 Copper glutarate 
                 250 
                 69 
                 0 
               
               
                 Copper malate 
                 250 
                 5 
                 20 
               
               
                 Copper succinate 
                 250 
                 50 
                 0 
               
               
                 Copper tartrate 
                 250 
                 69 
                 0 
               
               
                   
               
            
           
         
       
     
     As can be noted, the only compound that allows an almost complete control of the bacteriosis is copper salicylate in both crystalline form I and in crystalline form II, as it demonstrated an excellent bactericidal efficacy (5% of the leaf surface affected corresponding to an activity of 95%) without showing phytotoxicity phenomena, unlike copper malate which, on the other hand, proved to be phytotoxic (20%) at the same dosage of use.