Patent Publication Number: US-2023137139-A1

Title: Biosynthesis of cannabinoids and cannabinoid precursors

Description:
CROSS REFERENCE TO RELATED APPLICATION 
     This application claims the benefit under 35 U.S.C. § 119(e) of U.S. Provisional Application No. 63/000,419, filed Mar. 26, 2020, entitled “BIOSYNTHESIS OF CANNABINOIDS AND CANNABINOID PRECURSORS,” the entire disclosure of which is hereby incorporated by reference in its entirety. 
    
    
     REFERENCE TO A SEQUENCE LISTING SUBMITTED AS A TEXT FILE VIA EFS-WEB 
     The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. The ASCII file, created on Mar. 24, 2021, is named G091970059WO00-SEQ-OMJ.txt and is 526 kilobytes in size. 
     FIELD OF INVENTION 
     The present disclosure relates to the biosynthesis of cannabinoids and cannabinoid precursors, such as in recombinant cells. 
     BACKGROUND 
     Cannabinoids are chemical compounds that may act as ligands for endocannabinoid receptors and have multiple medical applications. Traditionally, cannabinoids have been isolated from plants of the genus  Cannabis . The use of plants for producing cannabinoids is inefficient, however, with isolated products often limited to the two most prevalent endogenous cannabinoids, THC and CBD, as other cannabinoids are typically produced in very low concentrations in  Cannabis  plants. Further, the cultivation of  Cannabis  plants is restricted in many jurisdictions. In addition, in order to obtain consistent results,  Cannabis  plants are often grown in a controlled environment, such as indoor grow rooms without windows, to provide flexibility in modulating growing conditions such as lighting, temperature, humidity, airflow, etc. Growing  Cannabis  plants in such controlled environments can result in high energy usage per gram of cannabinoid produced, especially for rare cannabinoids that the plants produce only in small amounts. For example, lighting in such grow rooms is provided by artificial sources, such as high-powered sodium lights. As many species of  Cannabis  have a vegetative cycle that requires 18 or more hours of light per day, powering such lights can result in significant energy expenditures. It has been estimated that between 0.88-1.34 kWh of energy is required to produce one gram of THC in dried  Cannabis  flower form (e.g., before any extraction or purification). Additionally, concern has been raised over agricultural practices in certain jurisdictions, such as California, where the growing season coincides with the dry season such that the water usage may impact connected surface water in streams (Dillis, Christopher, Connor McIntee, Van Butsic, Lance Le, Kason Grady, and Theodore Grantham. “Water storage and irrigation practices for cannabis drive seasonal patterns of water extraction and use in Northern California.” Journal of Environmental Management 272 (2020): 110955). 
     Cannabinoids can be produced through chemical synthesis (see, e.g., U.S. Pat. No. 7,323,576 to Souza et al). However, such methods suffer from low yields and high cost. Production of cannabinoids, cannabinoid analogs, and cannabinoid precursors using engineered organisms may provide an advantageous approach to meet the increasing demand for these compounds. 
     SUMMARY 
     Aspects of the present disclosure provide methods for production of cannabinoids and cannabinoid precursors from fatty acid substrates using genetically modified host cells. 
     Aspects of the disclosure relate to host cells that comprise a heterologous polynucleotide encoding a terminal synthase (TS), wherein the TS comprises a sequence that is at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical, or is 100% identical, to SEQ ID NO: 27 or 25 and wherein the host cell is capable of producing at least one cannabinoid. 
     Aspects of the disclosure relate to host cells that comprise a heterologous polynucleotide encoding a terminal synthase (TS), wherein the TS comprises a sequence that is at least 90% identical to SEQ ID NO: 27 or 25 and wherein the host cell is capable of producing at least one cannabinoid. 
     In some embodiments, relative to the sequence of SEQ ID NO: 27, the TS comprises an amino acid substitution at a residue corresponding to position 33, 39, 55, 57, 61, 62, 63, 71, 112, 122, 126, 129, 131 180, 183, 202, 256, 257, 260, 287, 295, 341, 386, 392, 394, 398, 410, 423, 426, 450, and/or 472 of SEQ ID NO: 27. 
     In some embodiments, the TS comprises: the amino acid D at a residue corresponding to position 33 in SEQ ID NO: 27; the amino acid F at a residue corresponding to position 39 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 55 in SEQ ID NO: 27; the amino acid Q or E at a residue corresponding to position 57 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 61 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 62 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 63 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 71 in SEQ ID NO: 27; the amino acid V or T at a residue corresponding to position 112 in SEQ ID NO: 27; the amino acid S, G, A or E at a residue corresponding to position 122 in SEQ ID NO: 27; the amino acid A, R, T, K, or D at a residue corresponding to position 126 in SEQ ID NO: 27; the amino acid W at a residue corresponding to position 129 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 131 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 180 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 183 in SEQ ID NO: 27; the amino acid S or G at a residue corresponding to position 202 in SEQ ID NO: 27; the amino acid F or M at a residue corresponding to position 256 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 257 in SEQ ID NO: 27; the amino acid M or F at a residue corresponding to position 260 in SEQ ID NO: 27; the amino acid R at a residue corresponding to position 287 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 295 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 341 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 386 in SEQ ID NO: 27; the amino acid H at a residue corresponding to position 392 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 394 in SEQ ID NO: 27; the amino acid F, T, A, or L at a residue corresponding to position 398 in SEQ ID NO: 27; the amino acid N at a residue corresponding to position 410 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 423 in SEQ ID NO: 27; the amino acid Y at a residue corresponding to position 426 in SEQ ID NO: 27; the amino acid K at a residue corresponding to position 450 in SEQ ID NO: 27; and/or the amino acid R or A at a residue corresponding to position 472 in SEQ ID NO: 27. 
     In some embodiments, the TS comprises one or more of the following amino acid substitutions relative to the sequence of SEQ ID NO: 27: T33D; Y39F; T55S; A57Q; A57E; G61A; V62I; V63I; Y71I; E112V; E112T; N122S; N122G; N122A; N122E; I126A; I126R; I126T; I126K; I126D; Y129W; N131S; S180T; R183T; N202S; N202G; Y256F; Y256M; N257S; V260M; V260F; H287R; N295S; A341S; V386A; L392H; M394T; V398F; V398T; V398A; V398L; D410N; S423A; H426Y; R450K; P472R; and/or P472A. 
     In some embodiments, the cannabinoid is a CBC-type cannabinoid. In some embodiments, the cannabinoid is cannabichromenic acid (CBCA) and/or cannabichromevarinic acid (CBCVA). In some embodiments, the host cell further produces one or more of tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA) and/or tetrahydrocannabivarinic acid (THCVA). 
     In some embodiments, the TS produces a higher ratio of CBCA:CBDA, CBCA:THCA, and/or CBCVA:THCVA than a control TS. In some embodiments, the control TS is a TS comprising the sequence of SEQ ID NO: 20, 23, 25 or 27. In some embodiments, the TS comprises one or more of the following amino acid substitutions relative to SEQ ID NO: 27: A57Q and G61A; Y71I; and/or V260F. In some embodiments, the TS has a higher product specificity for a CBC-type cannabinoid than a control TS. In some embodiments, the control TS is a TS comprising the sequence of SEQ ID NO: 20, 23, 25 or 27. In some embodiments, the TS comprises Y39F and/or V63I relative to the sequence of SEQ ID NO: 27. 
     In some embodiments, the TS comprises the sequence of any one of SEQ ID NOs: 25, 27, 105, 126, 134, 155, 162, 164, or 165, optionally wherein relative to the sequence of SEQ ID NO: 27, the TS comprises an amino acid substitution at a residue corresponding to position 33, 39, 55, 57, 61, 62, 63, 71, 112, 122, 126, 129, 131 180, 183, 202, 256, 257, 260, 287, 295, 341, 386, 392, 394, 398, 410, 423, 426, 450, and/or 472 of SEQ ID NO: 27. In some embodiments, the sequence of the TS comprises one or more of the following motifs: KVQARSGGH (SEQ ID NO: 174); RASNTQNQD[VI][FL]FA[VI]K (SEQ ID NO: 176); CPTI[KR]TGGH (SEQ ID NO: 181); WFVTLSLEGGAINDV[AP]EDATAY[AG]H (SEQ ID NO: 184); P[IV]S[DQE]TTY[EDG]F[TA]DGLYDVLA[RQK]AVPES[VA]GHAYLGCPDP[RK]M (SEQ ID NO: 186); MKHF[TNS]QFSM (SEQ ID NO: 189); P[EQ][TS]A[EAD][QE]IA[GA][VI]VKC (SEQ ID NO: 193); RDCL[IV]SA[LV]GGN[SA]A[LH][AV][AV]F[PQ][ND][QE]LL[WY] (SEQ ID NO: 200); RT[EQ][PQ]APGLAVQYSY (SEQ ID NO: 207); and/or WQ[SA]FI[SA][AQ][KE]NLT[RW][QK]FY[NST]NM (SEQ ID NO: 211). 
     Further aspects of the disclosure relate to host cells for producing a cannabinoid, wherein the host cell comprises a heterologous polynucleotide encoding a terminal synthase (TS), wherein the sequence of the TS comprises one or more of the following motifs: KVQARSGGH (SEQ ID NO: 174); RASNTQNQD[VI][FL]FA[VI]K (SEQ ID NO: 176); CPTI[KR]TGGH (SEQ ID NO: 181); WFVTLSLEGGAINDV[AP]EDATAY[AG]H (SEQ ID NO: 184); P[IV]S[DQE]TTY[EDG]F[TA]DGLYDVLA[RQK]AVPES[VA]GHAYLGCP DP[RK]M (SEQ ID NO: 186); MKHF[TNS]QFSM (SEQ ID NO: 189); P[EQ][TS]A[EAD][QE]IA[GA][VI]VKC (SEQ ID NO: 193); RDCL[IV]SA[LV]GGN[SA]A[LH][AV][AV]F[PQ][ND][QE]LL[WY] (SEQ ID NO: 200); RT[EQ][PQ]APGLAVQYSY (SEQ ID NO: 207); and/or WQ[SA]FI[SA][AQ][KE]NLT[RW][QK]FY[NST]NM (SEQ ID NO: 211), and wherein the host cell is capable of producing at least one cannabinoid. 
     In some embodiments, the motif KVQARSGGH (SEQ ID NO: 174) is located at residues in the TS corresponding to residues 72-80 in SEQ ID NO: 27; the motif RASNTQNQD[VI][FL]FA[VI]K (SEQ ID NO: 176) is located at residues in the TS corresponding to residues 183-197 in SEQ ID NO: 27; the motif CPTI[KR]TGGH (SEQ ID NO: 181) is located at residues in the TS corresponding to residues 141-149 in SEQ ID NO: 27; the motif WFVTLSLEGGAINDV[AP]EDATAY[AG]H (SEQ ID NO: 184) is located at residues in the TS corresponding to residues 360-383 in SEQ ID NO: 27; the motif P[IV]S[DQE]TTY[EDG]F[TA]DGLYDVLA[RQK]AVPES[VA]GHAYLGCPDP[RK]M (SEQ ID NO: 186) is located at residues in the TS corresponding to residues 400-436 in SEQ ID NO: 27; the motif MKHF[TNS]QFSM (SEQ ID NO: 189) is located at residues in the TS corresponding to residues 98-106 in SEQ ID NO: 27; the motif P[EQ][TS]A[EAD][QE]IA[GA][VI]VKC (SEQ ID NO: 193) is located at residues in the TS corresponding to residues 53-65 in SEQ ID NO: 27; the motif RDCL[IV]SA[LV]GGN[SA]A[LH][AV][AV]F[PQ][ND][QE]LL[WY] (SEQ ID NO: 200) is located at residues in the TS corresponding to residues 10-32 in SEQ ID NO: 27; the motif RT[EQ][PQ]APGLAVQYSY (SEQ ID NO: 207) is located at residues in the TS corresponding to residues 212-225 in SEQ ID NO: 27; and/or the motif WQ[SA]FI[SA][AQ][KE]NLT[RW][QK]FY[NST]NM (SEQ ID NO: 211) is located at residues in the TS corresponding to residues 242-259 in SEQ ID NO: 27. 
     In some embodiments, the TS is a fungal TS or a conservatively substituted version thereof. In some embodiments, the TS is an  Apergillus  TS or a conservatively substituted version thereof. In some embodiments, the TS comprises a sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172. In some embodiments, relative to the sequence of SEQ ID NO: 27, the TS comprises an amino acid substitution at a residue corresponding to position 33, 39, 55, 57, 61, 62, 63, 71, 112, 122, 126, 129, 131 180, 183, 202, 256, 257, 260, 287, 295, 341, 386, 392, 394, 398, 410, 423, 426, 450, and/or 472 of SEQ ID NO: 27. In some embodiments, the TS comprises: the amino acid D at a residue corresponding to position 33 in SEQ ID NO: 27; the amino acid F at a residue corresponding to position 39 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 55 in SEQ ID NO: 27; the amino acid Q or E at a residue corresponding to position 57 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 61 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 62 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 63 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 71 in SEQ ID NO: 27; the amino acid V or T at a residue corresponding to position 112 in SEQ ID NO: 27; the amino acid S, G, A or E at a residue corresponding to position 122 in SEQ ID NO: 27; the amino acid A, R, T, K, or D at a residue corresponding to position 126 in SEQ ID NO: 27; the amino acid W at a residue corresponding to position 129 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 131 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 180 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 183 in SEQ ID NO: 27; the amino acid S or G at a residue corresponding to position 202 in SEQ ID NO: 27; the amino acid F or M at a residue corresponding to position 256 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 257 in SEQ ID NO: 27; the amino acid M or F at a residue corresponding to position 260 in SEQ ID NO: 27; the amino acid R at a residue corresponding to position 287 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 295 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 341 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 386 in SEQ ID NO: 27; the amino acid H at a residue corresponding to position 392 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 394 in SEQ ID NO: 27; the amino acid F, T, A, or L at a residue corresponding to position 398 in SEQ ID NO: 27; the amino acid N at a residue corresponding to position 410 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 423 in SEQ ID NO: 27; the amino acid Y at a residue corresponding to position 426 in SEQ ID NO: 27; the amino acid K at a residue corresponding to position 450 in SEQ ID NO: 27; and/or the amino acid R or A at a residue corresponding to position 472 in SEQ ID NO: 27. 
     In some embodiments, the TS comprises one or more of the following amino acid substitutions relative to the sequence of SEQ ID NO: 27: T33D; Y39F; T55S; A57Q; A57E; G61A; V62I; V63I; Y71I; E112V; E112T; N122S; N122G; N122A; N122E; I126A; I126R; I126T; I126K; I126D; Y129W; N131S; S180T; R183T; N202S; N202G; Y256F; Y256M; N257S; V260M; V260F; H287R; N295S; A341S; V386A; L392H; M394T; V398F; V398T; V398A; V398L; D410N; S423A; H426Y; R450K; P472R; and/or P472A. In some embodiments, the TS comprises the sequence of any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 143, 144, 155, 159, 162-167, or 172 or a conservatively substituted version thereof. 
     Further aspects of the disclosure relate to host cells that comprises a heterologous polynucleotide encoding a terminal synthase (TS), wherein the TS comprises a sequence that is at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical, or is 100% identical, to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172, wherein the host cell is capable of producing at least one cannabinoid. 
     Further aspects of the disclosure relate to host cells that comprises a heterologous polynucleotide encoding a terminal synthase (TS), wherein the TS comprises a sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172, wherein the host cell is capable of producing at least one cannabinoid. 
     In some embodiments, the sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172 is linked to one or more signal peptides. In some embodiments, the sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172 is linked to a signal peptide that comprises SEQ ID NO: 16 or a sequence that has no more than two amino acid substitutions, insertions, additions, or deletions relative to the sequence of SEQ ID NO: 16. In some embodiments, the signal peptide is linked to the N-terminus of the sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172. In some embodiments, an N-terminal methionine is removed from SEQ ID NOs: 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172 and wherein a methionine residue is added to the N-terminus of the signal peptide. In some embodiments, the sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172 is linked to a signal peptide that comprises SEQ ID NO: 17 or a sequence that has no more than one amino acid substitution, insertion, addition, or deletion relative to the sequence of SEQ ID NO: 17. In some embodiments, the signal peptide that comprises SEQ ID NO: 17 or a sequence that has no more than one amino acid substitution, insertion, addition, or deletion relative to the sequence of SEQ ID NO: 17 is linked to the C-terminus of the sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172. 
     In some embodiments, relative to the sequence of SEQ ID NO: 27, the TS comprises an amino acid substitution at a residue corresponding to position 33, 39, 55, 57, 61, 62, 63, 71, 112, 122, 126, 129, 131 180, 183, 202, 256, 257, 260, 287, 295, 341, 386, 392, 394, 398, 410, 423, 426, 450, and/or 472 of SEQ ID NO: 27. In some embodiments, the TS comprises: the amino acid D at a residue corresponding to position 33 in SEQ ID NO: 27; the amino acid F at a residue corresponding to position 39 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 55 in SEQ ID NO: 27; the amino acid Q or E at a residue corresponding to position 57 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 61 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 62 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 63 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 71 in SEQ ID NO: 27; the amino acid V or T at a residue corresponding to position 112 in SEQ ID NO: 27; the amino acid S, G, A or E at a residue corresponding to position 122 in SEQ ID NO: 27; the amino acid A, R, T, K, or D at a residue corresponding to position 126 in SEQ ID NO: 27; the amino acid W at a residue corresponding to position 129 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 131 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 180 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 183 in SEQ ID NO: 27; the amino acid S or G at a residue corresponding to position 202 in SEQ ID NO: 27; the amino acid F or M at a residue corresponding to position 256 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 257 in SEQ ID NO: 27; the amino acid M or F at a residue corresponding to position 260 in SEQ ID NO: 27; the amino acid R at a residue corresponding to position 287 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 295 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 341 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 386 in SEQ ID NO: 27; the amino acid H at a residue corresponding to position 392 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 394 in SEQ ID NO: 27; the amino acid F, T, A, or L at a residue corresponding to position 398 in SEQ ID NO: 27; the amino acid N at a residue corresponding to position 410 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 423 in SEQ ID NO: 27; the amino acid Y at a residue corresponding to position 426 in SEQ ID NO: 27; the amino acid K at a residue corresponding to position 450 in SEQ ID NO: 27; and/or the amino acid R or A at a residue corresponding to position 472 in SEQ ID NO: 27. In some embodiments, the TS comprises one or more of the following amino acid substitutions relative to the sequence of SEQ ID NO: 27: T33D; Y39F; T55S; A57Q; A57E; G61A; V62I; V63I; Y71I; E112V; E112T; N122S; N122G; N122A; N122E; I126A; I126R; I126T; I126K; I126D; Y129W; N131S; S180T; R183T; N202S; N202G; Y256F; Y256M; N257S; V260M; V260F; H287R; N295S; A341S; V386A; L392H; M394T; V398F; V398T; V398A; V398L; D410N; S423A; H426Y; R450K; P472R; and/or P472A. 
     In some embodiments, the heterologous polynucleotide comprises a sequence that is at least 90% identical to any one of SEQ ID NOs: 26, 28, 35, 42, 56, 60, 64, 74, 85, 89, 92, 93, 94, 95, 96, 97, and 102. In some embodiments, the TS sequence comprises any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167 and 172. 
     Further aspects of the disclosure relate to host cells that comprise a heterologous polynucleotide encoding a terminal synthase (TS), wherein the TS comprises a sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172, or wherein the host cell comprises a conservatively substituted version of any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172. 
     Further aspects of the disclosure relate to host cells that comprise a heterologous polynucleotide encoding a terminal synthase (TS), wherein the host cell is capable of producing at least one cannabinoid, and wherein the TS is a fungal TS or a conservatively substituted version thereof. In some embodiments, the fungal TS is an  Aspergillus  TS or a conservatively substituted version thereof. In some embodiments, the cannabinoid is a is a CBC-type cannabinoid. In some embodiments, the cannabinoid is cannabichromenic acid (CBCA) and/or cannabichromevarinic acid (CBCVA). In some embodiments, the host cell further produces one or more of tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA) and/or tetrahydrocannabivarinic acid (THCVA). 
     In some embodiments, the host cell is a plant cell, an algal cell, a yeast cell, a bacterial cell, or an animal cell. In some embodiments, the host cell is a yeast cell. In some embodiments, the yeast cell is a  Saccharomyces  cell, a  Yarrowia  cell, a  Komagataella  cell, or a  Pichia  cell. In some embodiments, the  Saccharomyces  cell is a  Saccharomyces cerevisiae  cell. In some embodiments, the host cell is a bacterial cell. In some embodiments, the bacterial cell is an  E. coli  cell. In some embodiments, the host cell further comprises one or more heterologous polynucleotides encoding one or more of: an acyl activating enzyme (AAE), a polyketide synthase (PKS), a polyketide cyclase (PKC), a prenyltransferase (PT), and/or an additional terminal synthase (TS). In some embodiments, the PKS is an olivetol synthase (OLS) or a divarinol synthase. Further aspects of the disclosure relate to methods comprising culturing any of the host cells associated with the disclosure. 
     Further aspects of the disclosure relate to methods for producing a cannabinoid comprising contacting a CBG-type cannabinoid with a terminal synthase (TS), wherein the TS comprises a sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172. In some embodiments, contacting the CBG-type cannabinoid with the TS occurs in vitro. In some embodiments, contacting the CBG-type cannabinoid with the TS occurs in vivo. In some embodiments, contacting the CBG-type cannabinoid with the TS occurs in a host cell. Further aspects of the disclosure relate to methods for producing a cannabinoid comprising contacting a CBG-type cannabinoid in vivo with an oxidative cyclization catalyst adapted to preferentially convert the CBG-type cannabinoid to a CBC-type cannabinoid as compared to a CBD-type cannabinoid, a THC-type cannabinoid or both. 
     In some embodiments, the cannabinoid is a cyclized product of a CBG-type cannabinoid. In some embodiments, the cannabinoid is a cannabinoid with a cyclized prenyl moiety. In some embodiments, the cannabinoid is a CBC-type cannabinoid, a CBD-type cannabinoid, or a THC-type cannabinoid. In some embodiments, the cannabinoid is a CBC-type cannabinoid. In some embodiments, the CBG-type cannabinoid is cannabigerolic acid. In some embodiments, the CBC-type cannabinoid is CBCA. In some embodiments, the TS comprises the sequence of any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172 or a conservatively substituted version thereof. 
     Further aspects of the disclosure relate to host cells comprising a CBG-type cannabinoid and a means for catalyzing the oxidative cyclization of the CBG-type cannabinoid to preferentially convert the CBG-type cannabinoid to a CBC-type cannabinoid as compared to a CBG-type cannabinoid, a THC-type cannabinoid, or both. Further aspects of the disclosure relate to host cells comprising a CBG-type cannabinoid and an oxidative cyclization catalyst adapted to preferentially convert the CBG-type cannabinoid to a CBC-type cannabinoid as compared to a CBG-type cannabinoid, a THC-type cannabinoid, or both. In some embodiments, the means for catalyzing the oxidative cyclization of the CBG-type cannabinoid to produce a CBC-type cannabinoid is a heterologous polynucleotide encoding a terminal synthase (TS), wherein the TS comprises a sequence that is at least 90% identical to any of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172 or a conservatively substituted version thereof. In some embodiments, the TS is also capable of producing THCA, THCVA or CBDA. 
     Further aspects of the disclosure relate to non-naturally occurring nucleic acid encoding a terminal synthase (TS), wherein the non-naturally occurring nucleic acid comprises a sequence that has at least 90% identity to any one of SEQ ID NOs: 26, 28, 35, 42, 56, 60, 64, 74, 85, 89, 92, 93, 94, 95, 96, 97, and 102. Further aspects of the disclosure relate to vectors comprising non-naturally occurring nucleic acids associated with the disclosure. Further aspects of the disclosure relate to expression cassettes comprising non-naturally occurring nucleic acids associated with the disclosure. Further aspects of the disclosure relate to host cells transformed with non-naturally occurring nucleic acids, vectors, or expression cassettes associated with the disclosure. 
     Further aspects of the disclosure relate to bioreactors for producing a cannabinoid, wherein the bioreactor contains a CBG-type cannabinoid and a terminal synthase (TS), wherein the TS comprises a sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172 or wherein the TS comprises a conservatively substituted version of any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172. 
     Further aspects of the disclosure relate to non-naturally occurring terminal synthases (TS), wherein the TS comprises a sequence that is at least 90% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 144, 155, 159, 162-167, or 172. 
     Further aspects of the disclosure relate to oxidative cyclization catalysts adapted to preferentially convert a CBG-type cannabinoid to a CBC-type compound in vivo as compared to a THC-type compound or a CBD-type compound. 
     Each of the limitations of the invention can encompass various embodiments of the invention. It is, therefore, anticipated that each of the limitations of the invention involving any one element or combinations of elements can be included in each aspect of the invention. This disclosure is not limited in its application to the details of construction and the arrangement of components set forth in the following description or illustrated in the drawings. The invention is capable of other embodiments and of being practiced or of being carried out in various ways. Also, the phraseology and terminology used in this application is for the purpose of description and should not be regarded as limiting. The use of “including,” “comprising,” or “having,” “containing,” “involving,” and variations thereof, is meant to encompass the items listed thereafter and equivalents thereof as well as additional items. 
    
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
       The accompanying drawings are not intended to be drawn to scale. In the drawings, each identical or nearly identical component that is illustrated in various figures is represented by a like numeral. For purposes of clarity, not every component may be labeled in every drawing. In the drawings: 
         FIG.  1    is a schematic depicting the native  Cannabis  biosynthetic pathway for production of cannabinoid compounds, including five enzymatic steps mediated by: (Ria) acyl activating enzymes (AAE); (R2a) olivetol synthase enzymes (OLS); (R3a) olivetolic acid cyclase enzymes (OAC); (R4a) prenyltransferase enzymes (PT); and (R5a) terminal synthase enzymes (TS). Formulae 1a-11a correspond to hexanoic acid (1a), hexanoyl-CoA (2a), malonyl-CoA (3a), 3,5,7-trioxododecanoyl-CoA (4a), olivetol (5a), olivetolic acid (6a), geranyl pyrophosphate (7a), cannabigerolic acid (8a), cannabidiolic acid (9a), tetrahydrocannabinolic acid (10a), and cannabichromenic acid (11a). Hexanoic acid is an exemplary carboxylic acid substrate; other carboxylic acids may also be used (e.g., butyric acid, isovaleric acid, octanoic acid, decanoic acid, etc.; see e.g.,  FIG.  3    below). The enzymes that catalyze the synthesis of 3,5,7-trioxododecanoyl-CoA and olivetolic acid are shown in R2a and R3a, respectively, and can include multi-functional enzymes that catalyze the synthesis of 3,5,7-trioxododecanoyl-CoA and olivetolic acid. The enzymes cannabidiolic acid synthase (CBDAS), tetrahydrocannabinolic acid synthase (THCAS), and cannabichromenic acid synthase (CBCAS) that catalyze the synthesis of cannabidiolic acid, tetrahydrocannabinolic acid, and cannabichromenic acid, respectively, are shown in step R5a.  FIG.  1    is adapted from Carvalho et al. “Designing Microorganisms for Heterologous Biosynthesis of Cannabinoids” (2017)  FEMS Yeast Research  June 1; 17(4), which is incorporated by reference in its entirety. 
         FIG.  2    is a schematic depicting a heterologous biosynthetic pathway for production of cannabinoid compounds, including five enzymatic steps mediated by: (R1) acyl activating enzymes (AAE); (R2) polyketide synthase enzymes (PKS) or bifunctional polyketide synthase-polyketide cyclase enzymes (PKS-PKC); (R3) polyketide cyclase enzymes (PKC) or bifunctional PKS-PKC enzymes; (R4) prenyltransferase enzymes (PT); and (R5) terminal synthase enzymes (TS). Any carboxylic acid of varying chain lengths, structures (e.g., aliphatic, alicyclic, or aromatic) and functionalization (e.g., hydroxylic-, keto-, amino-, thiol-, aryl-, or alogeno-) may also be used as precursor substrates (e.g., thiopropionic acid, hydroxy phenyl acetic acid, norleucine, bromodecanoic acid, butyric acid, isovaleric acid, octanoic acid, decanoic acid, etc). 
         FIG.  3    is a non-exclusive representation of select putative precursors for the cannabinoid pathway in  FIG.  2   . 
         FIG.  4    is a schematic showing a reaction catalyzed by a TS enzyme wherein the geranyl moiety of cannabigerolic acid (Formula (8a)) is cyclized to yield cannabidiolic acid, tetrahydrocannabinolic acid, or cannabichromenic acid. 
         FIG.  5    is a schematic showing a plasmid bearing the transcriptional unit encoding a TS. The coding sequence for the TS enzymes (labeled “Library gene”) was driven by the GAL1 promoter. Each TS enzyme possessed an N-terminally fused  S. cerevisiae  Mating Factor alpha 2 signal peptide (labeled “MFα2”) and a C-terminally fused HDEL signal peptide (labeled “HDEL”). 
         FIG.  6    depicts a graph showing secondary screening data for CBCA production based on an in vivo activity assay in  S. cerevisiae . One library strain, strain t619896, expressing an  Aspergillus niger  ( A. niger ) CBCAS, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was observed to produce CBCA. Strain t616313, expressing GFP, was used as a negative control. Strain t616315, expressing a  C. sativa  THCAS, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control because it was observed to exhibit CBCAS activity as well as THCAS activity. The data represent the average of four biological replicates ±one standard deviation of the mean. Strain IDs and their corresponding activity from these graphs are shown in Table 5. 
         FIG.  7    depicts a graph showing production of CBCVA based on an in vivo activity assay in  S. cerevisiae  by library strain t619896. The data represent the average of four biological replicates ±one standard deviation of the mean. Strain IDs and their corresponding activity from these graphs are shown in Table 6. 
         FIGS.  8 A- 8 C  depict graphs showing secondary screening data of a library of TS variants for CBCA, THCA, and CBDA production based on an in vivo activity assay in  S. cerevisiae . Strain t865843, expressing a  C. sativa  THCAS, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control for THCAS activity. Strain t865768, expressing the  A. niger  CBCAS identified in Example 1, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control for CBCAS activity. Strain t876607, expressing a  C. sativa  CBDAS, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control for CBDAS activity. Strain t865842, expressing GFP, was used as a negative control. All library strains included an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide.  FIG.  8 A  depicts a graph showing CBCA production.  FIG.  8 B  depicts a graph showing THCA production.  FIG.  8 C  depicts a graph showing CBDA production. Strains depicted in  FIGS.  8 A- 8 C  and their corresponding activity are shown in Table 8. 
         FIGS.  9 A- 9 C  depict graphs showing secondary screening data of a library of TS variants for cannabichromevarinic acid (CBCVA), tetrahydrocannabivarinic acid (THCVA), and cannabidivarinic acid (CBDVA) production based on an in vivo activity assay in  S. cerevisiae . Strain t865843, expressing a  C. sativa  THCAS, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control for THCVAS activity. Strain t865768, expressing the  A. niger  CBCAS identified in Example 1, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control for CBCVAS activity. Strain t876607, expressing a  C. sativa  CBDAS, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control for CBDVAS activity. Strain t865842, expressing GFP, was used as a negative control. All library strains included an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide.  FIG.  9 A  depicts a graph showing CBCVA production.  FIG.  9 B  depicts a graph showing THCVA production.  FIG.  9 C  depicts a graph showing CBDVA production. Strains depicted in  FIGS.  9 A- 9 C  and their corresponding activity are shown in Table 9. 
         FIGS.  10 A- 10 C  depict graphs showing secondary screening activity data of candidate CBCAS enzymes identified in Example 3 for CBCA, THCA, and CBDA production based on an in vivo activity assay in  S. cerevisiae . Strain t807925, expressing the  A. niger  CBCAS identified in Example 1, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control for CBCAS activity. Strain t616313, expressing GFP, was used as a negative control. Strain t616314, expressing a  Cannabis  CBDAS, was used as a positive control for CBDAS activity. Strain t701870, expressing a  Cannabis  THCAS, was used as a positive control for THCAS activity. All library strains and positive control strains included an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide. The data represent the average of four biological replicates ±one standard deviation of the mean.  FIG.  10 A  depicts a graph showing CBCA production.  FIG.  10 B  depicts a graph showing THCA production.  FIG.  10 C  depicts a graph showing CBDA production. Strains depicted in  FIGS.  10 A- 10 C  and their corresponding activity are shown in Table 10. 
         FIGS.  11 A- 11 C  depict graphs showing secondary screening activity data of candidate CBCAS enzymes identified in Example 3 for CBCVA, THCVA, and CBDVA production based on an in vivo activity assay in  S. cerevisiae . Strain t807925, expressing the  A. niger  CBCAS identified in Example 1, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control. Strain t616313, expressing GFP, was used as a negative control. Strain t616314, expressing a  Cannabis  CBDAS, was used as a positive control. Strain t701870, expressing a  Cannabis  THCAS, was used as a positive control. All library strains and positive control strains included an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide. The data represent the average of four biological replicates ±one standard deviation of the mean.  FIG.  11 A  depicts a graph showing CBCVA production.  FIG.  11 B  depicts a graph showing THCVA production.  FIG.  11 C  depicts a graph showing CBDVA production. Strains depicted in  FIGS.  11 A- 11 C  and their corresponding activity are shown in Table 11. 
         FIGS.  12 A- 12 B  depict graphs showing substrate utilization of CBGA and CBGVA by candidate CBCAS enzymes identified in Example 3 based on an in vivo activity assay in  S. cerevisiae . Strain t807925, expressing the  A. niger  CBCAS identified in Example 1, including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control. Strain t616313, expressing GFP, was used as a negative control. All library strains included an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide. The data represent the average of four biological replicates ±one standard deviation of the mean.  FIG.  12 A  depicts a graph showing CBGA substrate utilization.  FIG.  12 B  depicts a graph showing CBGVA substrate utilization. Strains depicted in  FIGS.  12 A- 12 B  and their corresponding activity are shown in Table 12. 
         FIG.  13    depicts a percent identity matrix of candidate CBCAS enzymes identified in Examples 3 and 4. The far-left column and the top row recite SEQ ID NOs corresponding to specific enzymes. SEQ ID NO: 27 corresponds to the protein sequence associated with UniProt Accession No. A0A254UC34 from  A. niger . SEQ ID NO: 144 corresponds to the protein sequence associated with UniProt Accession No. A0A0C2SDS1, from  Amanita muscaria ; SEQ ID NO: 172 corresponds to the protein sequence associated with UniProt Accession No. B6HV04, from  Penicillium rubens ; SEQ ID NO: 166 corresponds to the protein sequence associated with UniProt Accession No. QOCYD9, from  Aspergillus terreus ; SEQ ID NO: 159 corresponds to the protein sequence associated with UniProt Accession No. A0A397IKU4, from  Aspergillus turcosus ; SEQ ID NO: 167 corresponds to the protein sequence associated with UniProt Accession No. A0A0K8LLN9, from  Aspergillus udagawae ; SEQ ID NO: 163 corresponds to the protein sequence associated with UniProt Accession NO. A0A2I1CBC7, from  Aspergillus novofumigatus ; SEQ ID NO: 165 corresponds to the protein sequence associated with UniProt Accession No. G3Y7J1, from  Aspergillus niger ; SEQ ID NO: 162 corresponds to the protein sequence associated with UniProt Accession No. A0A319AGI5, from  Aspergillus lacticoffeatus ; SEQ ID NO: 164 corresponds to the protein sequence associated with UniProt Accession No. A0A3F3PQ52, from  Aspergillus welwitschiae ; SEQ ID NO: 134 corresponds to the protein sequence associated with UniProt Accession No. A0A401KY63, from  Aspergillus awamori ; SEQ ID NO: 105 corresponds to the protein sequence associated with UniProt Accession No. A0A1L9NII2, from  Aspergillus tubingensis ; SEQ ID NO: 126 corresponds to the protein sequence associated with UniProt Accession No. A0A318Y6S9, from  Aspergillus neoniger ; SEQ ID NO: 155 corresponds to the protein sequence associated with UniProt Accession No. A0A319B6X5, from  Aspergillus  vadensis; SEQ ID NO: 112 corresponds to the protein sequence associated with UniProt Accession No. A0A0L1J4J1, from  Aspergillus nomiae ; and SEQ ID NO: 130 corresponds to the protein sequence associated with UniProt Accession No. Q2UF91, from  Aspergillus oryzae . The value in each cell in the matrix is the percent identity between the amino acid sequences of the enzymes of the corresponding X and Y axes. Cells with 100% percent identity are shaded in black with white text and cells with 95-99.99% identity are shaded in grey. 
         FIG.  14    depicts a graph showing secondary screening activity data of candidate CBCAS enzymes identified in Example 3 for CBCA production based on an in vivo activity assay in  S. cerevisiae . Strain 861555, expressing the  A. niger  CBCAS identified in Example 1 (referred to as “AnCBCAS”), including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide, was used as a positive control. Strain 861565 expresses the  A. niger  CBCAS identified in Example 1 (referred to as “AnCBCAS”) but excluding the N-terminally fused MFα2 signal peptide and the C-terminally fused HDEL signal peptide. All library strains were assayed in pairs with one strain including an N-terminally fused MFα2 signal peptide and a C-terminally fused HDEL signal peptide and the other strain excluding the N-terminally fused MFα2 signal peptide and C-terminally fused HDEL signal peptide. The data represent the average of four biological replicates ±one standard deviation of the mean. Strains depicted in  FIG.  14    and their corresponding activity are shown in Table 13. 
         FIG.  15    is a ribbon diagram depicting the predicted location within the 3-dimensional structure of a  Cannabis  TS of sequence motifs that were identified as being enriched in candidate non- Cannabis  CBCASs that were found to be effective in producing CBCA. Sequence motifs KVQARSGGH (SEQ ID NO: 174), CPTI[KR]TGGH (SEQ ID NO: 181), and P[IV]S[DQE]TTY[EDG]F[TA]DGLYDVLA[RQK]AVPES[VA]GHAYLGCPDP[RK]M (SEQ ID NO: 186), indicated by arrows, are predicted to contact the cofactor binding site. 
         FIG.  16    is a ribbon diagram depicting the predicted location within the 3-dimensional structure of a  Cannabis  TS of sequence motifs that were identified as being enriched in candidate non- Cannabis  CBCASs that were found to be effective in producing CBCA. The active site of the TS is shown in dark gray. The FAD cofactor is shown as sticks at the right-hand side of the diagram. The triangular void shown in the middle of the figure is the substrate binding site. The motifs RT[EQ][PQ]APGLAVQYSY (SEQ ID NO: 207) and WQ[SA]FI[SA][AQ][KE]NLT[RW][QK]FY[NST]NM (SEQ ID NO: 211), indicated by arrows, are predicted to be near the substrate binding pocket. 
     
    
    
     DETAILED DESCRIPTION 
     This disclosure provides methods for production of cannabinoids and cannabinoid precursors from fatty acid substrates using genetically modified host cells. Methods include heterologous expression of a terminal synthase (TS), such as a cannabichromenic acid synthase (CBCAS). The application describes TSs that can be functionally expressed in host cells such as  S. cerevisiae . As demonstrated in the Examples, multiple non- Cannabis  CBCASs were identified that were capable of producing cannabichromenic acid (CBCA) and cannabichromevarinic acid (CBCVA) in a host cell, as well as other TS products such as THCA, THCVA and CBDA. The TSs described in this disclosure may be useful in increasing the efficiency and purity of cannabinoid production such as, for example, by altering the activity and/or abundance of such enzymes. 
     Definitions 
     While the following terms are believed to be well understood by one of ordinary skill in the art, the following definitions are set forth to facilitate explanation of the disclosed subject matter. 
     The term “a” or “an” refers to one or more of an entity, i.e., can identify a referent as plural. Thus, the terms “a” or “an,” “one or more” and “at least one” are used interchangeably in this application. In addition, reference to “an element” by the indefinite article “a” or “an” does not exclude the possibility that more than one of the elements is present, unless the context clearly requires that there is one and only one of the elements. 
     The terms “microorganism” or “microbe” should be taken broadly. These terms are used interchangeably and include, but are not limited to, the two prokaryotic domains, Bacteria and Archaea, as well as certain eukaryotic fungi and protists. In some embodiments, the disclosure may refer to the “microorganisms” or “microbes” of lists/tables and figures present in the disclosure. This characterization can refer to not only the identified taxonomic genera of the tables and figures, but also the identified taxonomic species, as well as the various novel and newly identified or designed strains of any organism in the tables or figures. The same characterization holds true for the recitation of these terms in other parts of the specification, such as in the Examples. 
     The term “prokaryotes” is recognized in the art and refers to cells that contain no nucleus or other cell organelles. The prokaryotes are generally classified in one of two domains, the Bacteria and the Archaea. 
     “Bacteria” or “eubacteria” refers to a domain of prokaryotic organisms. Bacteria include at least 11 distinct groups as follows: (1) Gram-positive (gram+) bacteria, of which there are two major subdivisions: (a) high G+C group (Actinomycetes, Mycobacteria,  Micrococcus , others) and (b) low G+C group ( Bacillus, Clostridia, Lactobacillus , Staphylococci, Streptococci, Mycoplasmas); (2) Proteobacteria, e.g., Purple photosynthetic+non-photosynthetic Gram-negative bacteria (includes most “common” Gram-negative bacteria); (3) Cyanobacteria, e.g., oxygenic phototrophs; (4) Spirochetes and related species; (5) Planctomyces; (6)  Bacteroides , Flavobacteria; (7)  Chlamydia ; (8) Green sulfur bacteria; (9) Green non-sulfur bacteria (also anaerobic phototrophs); (10) Radioresistant micrococci and relatives; and (11)  Thermotoga  and  Thermosipho thermophiles.    
     The term “Archaea” refers to a taxonomic classification of prokaryotic organisms with certain properties that make them distinct from Bacteria in physiology and phylogeny. 
     The term “ Cannabis ” refers to a genus in the family Cannabaceae.  Cannabis  is a dioecious plant. Glandular structures located on female flowers of  Cannabis , called trichomes, accumulate relatively high amounts of a class of terpeno-phenolic compounds known as phytocannabinoids (described in further detail below).  Cannabis  has conventionally been cultivated for production of fibre and seed (commonly referred to as “hemp-type”), or for production of intoxicants (commonly referred to as “drug-type”). In drug-type  Cannabis , the trichomes contain relatively high amounts of tetrahydrocannabinolic acid (THCA), which can convert to tetrahydrocannabinol (THC) via a decarboxylation reaction, for example upon combustion of dried  Cannabis  flowers, to provide an intoxicating effect. Drug-type  Cannabis  often contains other cannabinoids in lesser amounts. In contrast, hemp-type  Cannabis  contains relatively low concentrations of THCA, often less than 0.3% THC by dry weight. Hemp-type  Cannabis  may contain non-THC and non-THCA cannabinoids, such as cannabidiolic acid (CBDA), cannabidiol (CBD), and other cannabinoids. Presently, there is a lack of consensus regarding the taxonomic organization of the species within the genus. Unless context dictates otherwise, the term “ Cannabis ” is intended to include all putative species within the genus, such as, without limitation,  Cannabis sativa, Cannabis indica , and  Cannabis ruderalis  and without regard to whether the  Cannabis  is hemp-type or drug-type. 
     The term “cyclase activity” in reference to a polyketide synthase (PKS) enzyme (e.g., an olivetol synthase (OLS) enzyme) or a polyketide cyclase (PKC) enzyme (e.g., an olivetolic acid cyclase (OAC) enzyme), refers to the activity of catalyzing the cyclization of an oxo fatty acyl-CoA (e.g., 3,5,7-trioxododecanoyl-COA, 3,5,7-trioxodecanoyl-COA) to the corresponding intramolecular cyclization product (e.g., olivetolic acid, divarinic acid). In some embodiments, the PKS or PKC catalyzes the C2-C7 aldol condensation of an acyl-COA with three additional ketide moieties added thereto. 
     A “cytosolic” or “soluble” enzyme refers to an enzyme that is predominantly localized (or predicted to be localized) in the cytosol of a host cell. 
     A “eukaryote” is any organism whose cells contain a nucleus and other organelles enclosed within membranes. Eukaryotes belong to the taxon Eukarya or Eukaryota. The defining feature that sets eukaryotic cells apart from prokaryotic cells (i.e., bacteria and archaea) is that they have membrane-bound organelles, especially the nucleus, which contains the genetic material, and is enclosed by the nuclear envelope. 
     The term “host cell” refers to a cell that can be used to express a polynucleotide, such as a polynucleotide that encodes an enzyme used in biosynthesis of cannabinoids or cannabinoid precursors. The terms “genetically modified host cell,” “recombinant host cell,” and “recombinant strain” are used interchangeably and refer to host cells that have been genetically modified by, e.g., cloning and transformation methods, or by other methods known in the art (e.g., selective editing methods, such as CRISPR). Thus, the terms include a host cell (e.g., bacterial cell, yeast cell, fungal cell, insect cell, plant cell, mammalian cell, human cell, etc.) that has been genetically altered, modified, or engineered, so that it exhibits an altered, modified, or different genotype and/or phenotype, as compared to the naturally-occurring cell from which it was derived. It is understood that in some embodiments, the terms refer not only to the particular recombinant host cell in question, but also to the progeny or potential progeny of such a host cell. 
     The term “control host cell,” or the term “control” when used in relation to a host cell, refers to an appropriate comparator host cell for determining the effect of a genetic modification or experimental treatment. In some embodiments, the control host cell is a wild type cell. In other embodiments, a control host cell is genetically identical to the genetically modified host cell, except for the genetic modification(s) differentiating the genetically modified or experimental treatment host cell. In some embodiments, the control host cell has been genetically modified to express a wild type or otherwise known variant of an enzyme being tested for activity in other test host cells. 
     The term “heterologous” with respect to a polynucleotide, such as a polynucleotide comprising a gene, is used interchangeably with the term “exogenous” and the term “recombinant” and refers to: a polynucleotide that has been artificially supplied to a biological system; a polynucleotide that has been modified within a biological system, or a polynucleotide whose expression or regulation has been manipulated within a biological system. A heterologous polynucleotide that is introduced into or expressed in a host cell may be a polynucleotide that comes from a different organism or species from the host cell, or may be a synthetic polynucleotide, or may be a polynucleotide that is also endogenously expressed in the same organism or species as the host cell. For example, a polynucleotide that is endogenously expressed in a host cell may be considered heterologous when it is situated non-naturally in the host cell; expressed recombinantly in the host cell, either stably or transiently; modified within the host cell; selectively edited within the host cell; expressed in a copy number that differs from the naturally occurring copy number within the host cell; or expressed in a non-natural way within the host cell, such as by manipulating regulatory regions that control expression of the polynucleotide. In some embodiments, a heterologous polynucleotide is a polynucleotide that is endogenously expressed in a host cell but whose expression is driven by a promoter that does not naturally regulate expression of the polynucleotide. In other embodiments, a heterologous polynucleotide is a polynucleotide that is endogenously expressed in a host cell and whose expression is driven by a promoter that does naturally regulate expression of the polynucleotide, but the promoter or another regulatory region is modified. In some embodiments, the promoter is recombinantly activated or repressed. For example, gene-editing based techniques may be used to regulate expression of a polynucleotide, including an endogenous polynucleotide, from a promoter, including an endogenous promoter. See, e.g., Chavez et al., Nat Methods. 2016 July; 13(7): 563-567. A heterologous polynucleotide may comprise a wild-type sequence or a mutant sequence as compared with a reference polynucleotide sequence. 
     The term “at least a portion” or “at least a fragment” of a nucleic acid or polypeptide means a portion having the minimal size characteristics of such sequences, or any larger fragment of the full length molecule, up to and including the full length molecule. A fragment of a polynucleotide of the disclosure may encode a biologically active portion of an enzyme, such as a catalytic domain. A biologically active portion of a genetic regulatory element may comprise a portion or fragment of a full length genetic regulatory element and have the same type of activity as the full length genetic regulatory element, although the level of activity of the biologically active portion of the genetic regulatory element may vary compared to the level of activity of the full length genetic regulatory element. 
     A coding sequence and a regulatory sequence are said to be “operably joined” or “operably linked” when the coding sequence and the regulatory sequence are covalently linked and the expression or transcription of the coding sequence is under the influence or control of the regulatory sequence. If the coding sequence is to be translated into a functional protein, the coding sequence and the regulatory sequence are said to be operably joined if induction of a promoter in the 5′ regulatory sequence promotes transcription of the coding sequence and if the nature of the linkage between the coding sequence and the regulatory sequence does not (1) result in the introduction of a frame-shift mutation, (2) interfere with the ability of the promoter region to direct the transcription of the coding sequence, or (3) interfere with the ability of the corresponding RNA transcript to be translated into a protein. 
     The terms “link,” “linked,” or “linkage” means two entities (e.g., two polynucleotides or two proteins) are bound to one another by any physicochemical means. Any linkage known to those of ordinary skill in the art, covalent or non-covalent, is embraced. In some embodiments, a nucleic acid sequence encoding an enzyme of the disclosure is linked to a nucleic acid encoding a signal peptide. In some embodiments, an enzyme of the disclosure is linked to a signal peptide. Linkage can be direct or indirect. 
     The terms “transformed” or “transform” with respect to a host cell refer to a host cell in which one or more nucleic acids have been introduced, for example on a plasmid or vector or by integration into the genome. In some instances where one or more nucleic acids are introduced into a host cell on a plasmid or vector, one or more of the nucleic acids, or fragments thereof, may be retained in the cell, such as by integration into the genome of the cell, while the plasmid or vector itself may be removed from the cell. In such instances, the host cell is considered to be transformed with the nucleic acids that were introduced into the cell regardless of whether the plasmid or vector is retained in the cell or not. 
     The term “volumetric productivity” or “production rate” refers to the amount of product formed per volume of medium per unit of time. Volumetric productivity can be reported in gram per liter per hour (g/L/h). 
     The term “specific productivity” of a product refers to the rate of formation of the product normalized by unit volume or mass or biomass and has the physical dimension of a quantity of substance per unit time per unit mass or volume [M·T −1 ·M −1  or M·T −1 ·L −3 , where M is mass or moles, T is time, L is length]. 
     The term “biomass specific productivity” refers to the specific productivity in gram product per gram of cell dry weight (CDW) per hour (g/g CDW/h) or in mmol of product per gram of cell dry weight (CDW) per hour (mmol/g CDW/h). Using the relation of CDW to OD600 for the given microorganism, specific productivity can also be expressed as gram product per liter culture medium per optical density of the culture broth at 600 nm (OD) per hour (g/L/h/OD). Also, if the elemental composition of the biomass is known, biomass specific productivity can be expressed in mmol of product per C-mole (carbon mole) of biomass per hour (mmol/C-mol/h). 
     The term “yield” refers to the amount of product obtained per unit weight of a certain substrate and may be expressed as g product per g substrate (g/g) or moles of product per mole of substrate (mol/mol). Yield may also be expressed as a percentage of the theoretical yield. “Theoretical yield” is defined as the maximum amount of product that can be generated per a given amount of substrate as dictated by the stoichiometry of the metabolic pathway used to make the product and may be expressed as g product per g substrate (g/g) or moles of product per mole of substrate (mol/mol). 
     The term “titer” refers to the strength of a solution or the concentration of a substance in solution. For example, the titer of a product of interest (e.g., small molecule, peptide, synthetic compound, fuel, alcohol, etc.) in a fermentation broth is described as g of product of interest in solution per liter of fermentation broth or cell-free broth (g/L) or as g of product of interest in solution per kg of fermentation broth or cell-free broth (g/Kg). 
     The term “total titer” refers to the sum of all products of interest produced in a process, including but not limited to the products of interest in solution, the products of interest in gas phase if applicable, and any products of interest removed from the process and recovered relative to the initial volume in the process or the operating volume in the process. For example, the total titer of products of interest (e.g., small molecule, peptide, synthetic compound, fuel, alcohol, etc.) in a fermentation broth is described as g of products of interest in solution per liter of fermentation broth or cell-free broth (g/L) or as g of products of interest in solution per kg of fermentation broth or cell-free broth (g/Kg). 
     The term “amino acid” refers to organic compounds that comprise an amino group, —NH2, and a carboxyl group, —COOH. The term “amino acid” includes both naturally occurring and unnatural amino acids. Nomenclature for the twenty common amino acids is as follows: alanine (ala or A); arginine (arg or R); asparagine (asn or N); aspartic acid (asp or D); cysteine (cys or C); glutamine (gln or Q); glutamic acid (glu or E); glycine (gly or G); histidine (his or H); isoleucine (ile or I); leucine (leu or L); lysine (lys or K); methionine (met or M); phenylalanine (phe or F); proline (pro or P); serine (ser or S); threonine (thr or T); tryptophan (trp or W); tyrosine (tyr or Y); and valine (val or V). Non-limiting examples of unnatural amino acids include homo-amino acids, proline and pyruvic acid derivatives, 3-substituted alanine derivatives, glycine derivatives, ring-substituted phenylalanine derivatives, ring-substituted tyrosine derivatives, linear core amino acids, amino acids with protecting groups including Fmoc, Boc, and Cbz, j-amino acids (β3 and β2), and N-methyl amino acids. 
     The term “aliphatic” refers to alkyl, alkenyl, alkynyl, and carbocyclic groups. Likewise, the term “heteroaliphatic” refers to heteroalkyl, heteroalkenyl, heteroalkynyl, and heterocyclic groups. 
     The term “alkyl” refers to a radical of, or a substituent that is, a straight-chain or branched saturated hydrocarbon group having from 1 to 20 carbon atoms (“C1-20 alkyl”). In certain embodiments, the term “alkyl” refers to a radical of, or a substituent that is, a straight-chain or branched saturated hydrocarbon group having from 1 to 10 carbon atoms (“C 1-10  alkyl”). In some embodiments, an alkyl group has 1 to 9 carbon atoms (“C 1-9  alkyl”). In some embodiments, an alkyl group has 1 to 8 carbon atoms (“C 1-8  alkyl”). In some embodiments, an alkyl group has 1 to 7 carbon atoms (“C 1-7  alkyl”). In some embodiments, an alkyl group has 2 to 7 carbon atoms (“C2-7 alkyl”). In some embodiments, an alkyl group has 3 to 7 carbon atoms (“C3-7 alkyl”). In some embodiments, an alkyl group has 1 to 6 carbon atoms (“C 1-6  alkyl”). In some embodiments, an alkyl group has 2 to 6 carbon atoms (“C 2-6  alkyl”). In some embodiments, an alkyl group has 3 to 5 carbon atoms (“C 3-5  alkyl”). In some embodiments, an alkyl group has 5 carbon atoms (“C 5  alkyl”). In some embodiments, the alkyl group has 3 carbon atoms (“C3 alkyl”). In some embodiments, the alkyl group has 7 carbon atoms (“C7 alkyl”). In some embodiments, an alkyl group has 1 to 5 carbon atoms (“C 1-5  alkyl”). In some embodiments, an alkyl group has 1 to 4 carbon atoms (“C 1-4  alkyl”). In some embodiments, an alkyl group has 1 to 3 carbon atoms (“C 1-3  alkyl”). In some embodiments, an alkyl group has 1 to 2 carbon atoms (“C 1-2  alkyl”). In some embodiments, an alkyl group has 1 carbon atom (“C 1  alkyl”). 
     Examples of C 1-6  alkyl groups include methyl (C 1 ), ethyl (C 2 ), propyl (C 3 ) (e.g., n-propyl, isopropyl), butyl (C 4 ) (e.g., n-butyl, tert-butyl, sec-butyl, iso-butyl), pentyl (C 5 ) (e.g., n-pentyl, 3-pentanyl, amyl, neopentyl, 3-methyl-2-butanyl, tertiary amyl), and hexyl (C 6 ) (e.g., n-hexyl). Additional examples of alkyl groups include n-heptyl (C 7 ), n-octyl (C 8 ), and the like. Unless otherwise specified, each instance of an alkyl group is independently unsubstituted (an “unsubstituted alkyl”) or substituted (a “substituted alkyl”) with one or more substituents (e.g., halogen, such as F). In certain embodiments, the alkyl group is an unsubstituted C 1-10  alkyl (such as unsubstituted C 1-6  alkyl, e.g., —CH 3  (Me), unsubstituted ethyl (Et), unsubstituted propyl (Pr, e.g., unsubstituted n-propyl (n-Pr), unsubstituted isopropyl (i-Pr)), unsubstituted butyl (Bu, e.g., unsubstituted n-butyl (n-Bu), unsubstituted tert-butyl (tert-Bu or t-Bu), unsubstituted sec-butyl (sec-Bu), unsubstituted isobutyl (i-Bu)). In certain embodiments, the alkyl group is a substituted C 1-10  alkyl (such as substituted C 1-6  alkyl, e.g., —CF 3 , benzyl). 
     The term “acyl” refers to a group having the general formula —C(═O)R X1 , —C(═O)OR X1 , —C(═O)—O—C(═O)R X1 , —C(═O)SR X1 , —C(═O)N(R X1 ) 2 , —C(═S)R X1 , —C(═S)N(R X1 ) 2 , and —C(═S)S(R X1 ), —C(═NR X1 )R X1 , —C(═NR X1 )OR X1 , —C(═NR X1 )SR X1 , and —C(═NR X1 )N(R X1 ) 2 , wherein R X1  is hydrogen; halogen; substituted or unsubstituted hydroxyl; substituted or unsubstituted thiol; substituted or unsubstituted amino; substituted or unsubstituted acyl, cyclic or acyclic, substituted or unsubstituted, branched or unbranched aliphatic; cyclic or acyclic, substituted or unsubstituted, branched or unbranched heteroaliphatic; cyclic or acyclic, substituted or unsubstituted, branched or unbranched alkyl; cyclic or acyclic, substituted or unsubstituted, branched or unbranched alkenyl; substituted or unsubstituted alkynyl; substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, aliphaticoxy, heteroaliphaticoxy, alkyloxy, heteroalkyloxy, aryloxy, heteroaryloxy, aliphaticthioxy, heteroaliphaticthioxy, alkylthioxy, heteroalkylthioxy, arylthioxy, heteroarylthioxy, mono- or di-aliphaticamino, mono- or di-heteroaliphaticamino, mono- or di-alkylamino, mono- or di-heteroalkylamino, mono- or di-arylamino, or mono- or di-heteroarylamino; or two R X1  groups taken together form a 5- to 6-membered heterocyclic ring. Exemplary acyl groups include aldehydes (—CHO), carboxylic acids (—CO 2 H), ketones, acyl halides, esters, amides, imines, carbonates, carbamates, and ureas. Acyl substituents include, but are not limited to, any of the substituents described in this application that result in the formation of a stable moiety (e.g., aliphatic, alkyl, alkenyl, alkynyl, heteroaliphatic, heterocyclic, aryl, heteroaryl, acyl, oxo, imino, thiooxo, cyano, isocyano, amino, azido, nitro, hydroxyl, thiol, halo, aliphaticamino, heteroaliphaticamino, alkylamino, heteroalkylamino, arylamino, heteroarylamino, alkylaryl, arylalkyl, aliphaticoxy, heteroaliphaticoxy, alkyloxy, heteroalkyloxy, aryloxy, heteroaryloxy, aliphaticthioxy, heteroaliphaticthioxy, alkylthioxy, heteroalkylthioxy, arylthioxy, heteroarylthioxy, acyloxy, and the like, each of which may or may not be further substituted). 
     “Alkenyl” refers to a radical of, or a substituent that is, a straight-chain or branched hydrocarbon group having from 2 to 20 carbon atoms, one or more carbon-carbon double bonds, and no triple bonds (“C 2-20  alkenyl”). In some embodiments, an alkenyl group has 2 to 10 carbon atoms (“C 2-10  alkenyl”). In some embodiments, an alkenyl group has 2 to 9 carbon atoms (“C 2-9  alkenyl”). In some embodiments, an alkenyl group has 2 to 8 carbon atoms (“C 2-8  alkenyl”). In some embodiments, an alkenyl group has 2 to 7 carbon atoms (“C 2-7  alkenyl”). In some embodiments, an alkenyl group has 2 to 6 carbon atoms (“C 2-6  alkenyl”). In some embodiments, an alkenyl group has 2 to 5 carbon atoms (“C 2-5  alkenyl”). In some embodiments, an alkenyl group has 2 to 4 carbon atoms (“C 2-4  alkenyl”). In some embodiments, an alkenyl group has 2 to 3 carbon atoms (“C 2-3  alkenyl”). In some embodiments, an alkenyl group has 2 carbon atoms (“C 2  alkenyl”). The one or more carbon-carbon double bonds can be internal (such as in 2-butenyl) or terminal (such as in 1-butenyl). Examples of C 2-4  alkenyl groups include ethenyl (C 2 ), 1-propenyl (C 3 ), 2-propenyl (C 3 ), 1-butenyl (C 4 ), 2-butenyl (C 4 ), butadienyl (C 4 ), and the like. Examples of C 2-6  alkenyl groups include the aforementioned C 2-4  alkenyl groups as well as pentenyl (C 5 ), pentadienyl (C 5 ), hexenyl (C 6 ), and the like. Additional examples of alkenyl include heptenyl (C 7 ), octenyl (C 8 ), octatrienyl (C 8 ), and the like. Unless otherwise specified, each instance of an alkenyl group is independently optionally substituted, i.e., unsubstituted (an “unsubstituted alkenyl”) or substituted (a “substituted alkenyl”) with one or more substituents. In certain embodiments, the alkenyl group is unsubstituted C 2-10  alkenyl. In certain embodiments, the alkenyl group is substituted C 2-10  alkenyl. 
     “Alkynyl” refers to a radical of, or a substituent that is, a straight-chain or branched hydrocarbon group having from 2 to 20 carbon atoms, one or more carbon-carbon triple bonds, and optionally one or more double bonds (“C 2-20  alkynyl”). In some embodiments, an alkynyl group has 2 to 10 carbon atoms (“C 2-10  alkynyl”). In some embodiments, an alkynyl group has 2 to 9 carbon atoms (“C 2-9  alkynyl”). In some embodiments, an alkynyl group has 2 to 8 carbon atoms (“C 2-8  alkynyl”). In some embodiments, an alkynyl group has 2 to 7 carbon atoms (“C 2-7  alkynyl”). In some embodiments, an alkynyl group has 2 to 6 carbon atoms (“C 2-6  alkynyl”). In some embodiments, an alkynyl group has 2 to 5 carbon atoms (“C 2-5  alkynyl”). In some embodiments, an alkynyl group has 2 to 4 carbon atoms (“C 2-4  alkynyl”). In some embodiments, an alkynyl group has 2 to 3 carbon atoms (“C 2-3  alkynyl”). In some embodiments, an alkynyl group has 2 carbon atoms (“C 2  alkynyl”). The one or more carbon-carbon triple bonds can be internal (such as in 2-butynyl) or terminal (such as in 1-butynyl). Examples of C 2-4  alkynyl groups include, without limitation, ethynyl (C 2 ), 1-propynyl (C 3 ), 2-propynyl (C 3 ), 1-butynyl (C 4 ), 2-butynyl (C 4 ), and the like. Examples of C 2-6  alkenyl groups include the aforementioned C 2-4  alkynyl groups as well as pentynyl (C 5 ), hexynyl (C), and the like. Additional examples of alkynyl include heptynyl (C 7 ), octynyl (C 8 ), and the like. Unless otherwise specified, each instance of an alkynyl group is independently optionally substituted, i.e., unsubstituted (an “unsubstituted alkynyl”) or substituted (a “substituted alkynyl”) with one or more substituents. In certain embodiments, the alkynyl group is unsubstituted C 2-10  alkynyl. In certain embodiments, the alkynyl group is substituted C 2-10  alkynyl. 
     “Carbocyclyl” or “carbocyclic” refers to a radical of a non-aromatic cyclic hydrocarbon group having from 3 to 10 ring carbon atoms (“C 3-10  carbocyclyl”) and zero heteroatoms in the non-aromatic ring system. In some embodiments, a carbocyclyl group has 3 to 8 ring carbon atoms (“C 3-8  carbocyclyl”). In some embodiments, a carbocyclyl group has 3 to 6 ring carbon atoms (“C 3-6  carbocyclyl”). In some embodiments, a carbocyclyl group has 3 to 6 ring carbon atoms (“C 3-6  carbocyclyl”). In some embodiments, a carbocyclyl group has 5 to 10 ring carbon atoms (“C 5-10  carbocyclyl”). Exemplary C 3-6  carbocyclyl groups include, without limitation, cyclopropyl (C 3 ), cyclopropenyl (C 3 ), cyclobutyl (C 4 ), cyclobutenyl (C 4 ), cyclopentyl (C 5 ), cyclopentenyl (C 5 ), cyclohexyl (C), cyclohexenyl (C), cyclohexadienyl (C), and the like. Exemplary C 3-8  carbocyclyl groups include, without limitation, the aforementioned C 3-6  carbocyclyl groups as well as cycloheptyl (C 7 ), cycloheptenyl (C 7 ), cycloheptadienyl (C 7 ), cycloheptatrienyl (C 7 ), cyclooctyl (C 8 ), cyclooctenyl (C 8 ), bicyclo[2.2.1]heptanyl (C 7 ), bicyclo[2.2.2]octanyl (C 8 ), and the like. Exemplary C 3-10  carbocyclyl groups include, without limitation, the aforementioned C 3-8  carbocyclyl groups as well as cyclononyl (C 9 ), cyclononenyl (C 9 ), cyclodecyl (C 10 ), cyclodecenyl (C 10 ), octahydro-1H-indenyl (C 9 ), decahydronaphthalenyl (C 10 ), spiro[4.5]decanyl (C 10 ), and the like. As the foregoing examples illustrate, in certain embodiments, the carbocyclyl group is either monocyclic (“monocyclic carbocyclyl”) or contain a fused, bridged or spiro ring system such as a bicyclic system (“bicyclic carbocyclyl”) and can be saturated or can be partially unsaturated. “Carbocyclyl” also includes ring systems wherein the carbocyclic ring, as defined above, is fused with one or more aryl or heteroaryl groups wherein the point of attachment is on the carbocyclic ring, and in such instances, the number of carbons continue to designate the number of carbons in the carbocyclic ring system. Unless otherwise specified, each instance of a carbocyclyl group is independently optionally substituted, i.e., unsubstituted (an “unsubstituted carbocyclyl”) or substituted (a “substituted carbocyclyl”) with one or more substituents. In certain embodiments, the carbocyclyl group is unsubstituted C 3-10  carbocyclyl. In certain embodiments, the carbocyclyl group is a substituted C 3-10  carbocyclyl. 
     In some embodiments, “carbocyclyl” is a monocyclic, saturated carbocyclyl group having from 3 to 10 ring carbon atoms (“C 3-10  cycloalkyl”). In some embodiments, a cycloalkyl group has 3 to 8 ring carbon atoms (“C 3-8  cycloalkyl”). In some embodiments, a cycloalkyl group has 3 to 6 ring carbon atoms (“C 3-6  cycloalkyl”). In some embodiments, a cycloalkyl group has 5 to 6 ring carbon atoms (“C 5-6  cycloalkyl”). In some embodiments, a cycloalkyl group has 5 to 10 ring carbon atoms (“C 5-10  cycloalkyl”). Examples of C 5-6  cycloalkyl groups include cyclopentyl (C 5 ) and cyclohexyl (C 5 ). Examples of C 3-6  cycloalkyl groups include the aforementioned C 5-6  cycloalkyl groups as well as cyclopropyl (C 3 ) and cyclobutyl (C 4 ). Examples of C 3-8  cycloalkyl groups include the aforementioned C 3-6  cycloalkyl groups as well as cycloheptyl (C 7 ) and cyclooctyl (C 8 ). Unless otherwise specified, each instance of a cycloalkyl group is independently unsubstituted (an “unsubstituted cycloalkyl”) or substituted (a “substituted cycloalkyl”) with one or more substituents. In certain embodiments, the cycloalkyl group is unsubstituted C 3-10  cycloalkyl. In certain embodiments, the cycloalkyl group is substituted C 3-10  cycloalkyl. 
     “Aryl” refers to a radical of a monocyclic or polycyclic (e.g., bicyclic or tricyclic) 4n+2 aromatic ring system (e.g., having 6, 10, or 14 pi electrons shared in a cyclic array) having 6-14 ring carbon atoms and zero heteroatoms provided in the aromatic ring system (“C 6-14  aryl”). In some embodiments, an aryl group has six ring carbon atoms (“C 6  aryl”; e.g., phenyl). In some embodiments, an aryl group has ten ring carbon atoms (“C 10  aryl”; e.g., naphthyl such as 1-naphthyl and 2-naphthyl). In some embodiments, an aryl group has fourteen ring carbon atoms (“C 14  aryl”; e.g., anthracyl). “Aryl” also includes ring systems wherein the aryl ring, as defined above, is fused with one or more carbocyclyl or heterocyclyl groups wherein the radical or point of attachment is on the aryl ring, and in such instances, the number of carbon atoms continue to designate the number of carbon atoms in the aryl ring system. Unless otherwise specified, each instance of an aryl group is independently optionally substituted, i.e., unsubstituted (an “unsubstituted aryl”) or substituted (a “substituted aryl”) with one or more substituents. In certain embodiments, the aryl group is unsubstituted C 6-14  aryl. In certain embodiments, the aryl group is substituted C 6-14  aryl. 
     “Aralkyl” is a subset of alkyl and aryl and refers to an optionally substituted alkyl group substituted by an optionally substituted aryl group. In certain embodiments, the aralkyl is optionally substituted benzyl. In certain embodiments, the aralkyl is benzyl. In certain embodiments, the aralkyl is optionally substituted phenethyl. In certain embodiments, the aralkyl is phenethyl. In certain embodiments, the aralkyl is 7-phenylheptanyl. In certain embodiments, the aralkyl is C7 alkyl substituted by an optionally substituted aryl group (e.g., phenyl). In certain embodiments, the aralkyl is a C7-C10 alkyl group substituted by an optionally substituted aryl group (e.g., phenyl). 
     “Partially unsaturated” refers to a group that includes at least one double or triple bond. A “partially unsaturated” ring system is further intended to encompass rings having multiple sites of unsaturation but is not intended to include aromatic groups (e.g., aryl or heteroaryl groups) as defined in this application. Likewise, “saturated” refers to a group that does not contain a double or triple bond, i.e., contains all single bonds. 
     The term “optionally substituted” means substituted or unsubstituted. 
     Alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl groups are optionally substituted (e.g., “substituted” or “unsubstituted” alkyl, “substituted” or “unsubstituted” alkenyl, “substituted” or “unsubstituted” alkynyl, “substituted” or “unsubstituted” carbocyclyl, “substituted” or “unsubstituted” heterocyclyl, “substituted” or “unsubstituted” aryl or “substituted” or “unsubstituted” heteroaryl group). In general, the term “substituted,” whether preceded by the term “optionally” or not, means that at least one hydrogen present on a group (e.g., a carbon or nitrogen atom) is replaced with a permissible substituent, e.g., a substituent which upon substitution results in a stable compound, e.g., a compound which does not spontaneously undergo transformation such as by rearrangement, cyclization, elimination, or other reaction. Unless otherwise indicated, a “substituted” group has a substituent at one or more substitutable positions of the group, and when more than one position in any given structure is substituted, the substituent is either the same or different at each position. The term “substituted” is contemplated to include substitution with all permissible substituents of organic compounds, any of the substituents described in this application that results in the formation of a stable compound. The present invention contemplates any and all such combinations in order to arrive at a stable compound. For purposes of this invention, heteroatoms such as nitrogen may have hydrogen substituents and/or any suitable substituent as described in this application which satisfy the valencies of the heteroatoms and results in the formation of a stable moiety. 
     Exemplary carbon atom substituents include, but are not limited to, halogen, —CN, —NO 2 , —N 3 , —SO 2 H, —SO 3 H, —OH, —OR aa , —ON(R bb ) 2 , —N(R bb ) 2 , —N(R bb ) 3 +X − , —N(OR cc )R bb , —SH, —SR aa , —SSR cc , —C(═O)R aa , —CO 2 H, —CHO, —C(OR cc ) 2 , —CO 2 R aa , —OC(═O)R aa , —OCO 2 R aa , —C(═O)N(R bb ) 2 , —OC(═O)N(R bb ) 2 , —NR bb C(═O)R aa , —NR bb CO 2 R aa , —NR bb C(═O)N(R bb ) 2 , —C(═NR bb )R aa , —C(═NR bb )OR aa , —OC(═NR bb )R aa , —OC(═NR bb )OR aa , —C(═NR bb )N(R bb ) 2 , —OC(═NR bb )N(R bb ) 2 , —NR bb C(═NR bb )N(R bb ) 2 , —C(═O)NR bb SO 2 R aa , —NR bb SO 2 R aa , —SO 2 N(R bb ) 2 , —SO 2 R aa , —SO 2 OR aa , —OSO 2 R aa , —S(═O)R aa , —OS(═O)R aa , —Si(R aa ) 3 , —OSi(R aa ) 3  —C(═S)N(R bb ) 2 , —C(═O)SR aa , —C(═S)SR aa , —SC(═S)SR aa , —SC(═O)SR aa , —OC(═O)SR aa , —SC(═O)OR aa , —SC(═O)R aa , —P(═O)(R aa ) 2 , —P(═O)(OR cc ) 2 , —OP(═O)(R aa ) 2 , —OP(═O)(OR cc ) 2 , —P(═O)(N(R bb ) 2 ) 2 , —OP(═O)(N(R bb ) 2 ) 2 , —NR bb P(═O)(R aa ) 2 , —NR bb P(═O)(OR cc ) 2 , —NR bb P(═O)(N(R bb ) 2 ) 2 , —P(R cc ) 2 , —P(OR cc ) 2 , —P(R cc ) 3 +X − , —P(OR cc ) 3 +X − , —P(R cc ) 4 , —P(OR cc ) 4 , —OP(R cc ) 2 , —OP(R cc ) 3 +X − , —OP(OR cc ) 2 , —OP(OR cc ) 3 +X − , —OP(R cc ) 4 , —OP(OR cc ) 4 , —B(R aa ) 2 , —B(OR cc ) 2 , —BR aa (OR cc ), C 1-10  alkyl, C 1-10  perhaloalkyl, C 2-10  alkenyl, C 2-10  alkynyl, heteroC 1-10  alkyl, heteroC 2-10  alkenyl, heteroC 2-10  alkynyl, C 3-10  carbocyclyl, 3-14 membered heterocyclyl, C 6-14  aryl, and 5-14 membered heteroaryl; 
     wherein: 
     each instance of R aa  is, independently, selected from C 1-10  alkyl, C 1-10  perhaloalkyl, C 2-10  alkenyl, C 2-10  alkynyl, heteroC 1-10  alkyl, heteroC 2-10 alkenyl, heteroC 2-10 alkynyl, C 3-10  carbocyclyl, 3-14 membered heterocyclyl, C 6-14  aryl, and 5-14 membered heteroaryl, or two R aa  groups are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R dd  groups; 
     each instance of R bb  is, independently, selected from hydrogen, —OH, —OR aa , —N(R cc ) 2 , —CN, —C(═O)R aa , —C(═O)N(R cc ) 2 , —CO 2 R aa , —SO 2 R aa , —C(═NR cc )OR aa , —C(═NR cc )N(R cc ) 2 , —SO 2 N(R cc ) 2 , —SO 2 R cc , —SO 2 OR cc , —SOR aa , —C(═S)N(R cc ) 2 , —C(═O)SR cc , —C(═S)SR cc , —P(═O)(R aa ) 2 , —P(═O)(OR cc ) 2 , —P(═O)(N(R cc ) 2 ) 2 , C 1-10  alkyl, C 1-10  perhaloalkyl, C 2-10  alkenyl, C 2-10  alkynyl, heteroC 1-10 alkyl, heteroC 2-10 alkenyl, heteroC 2-10 alkynyl, C 3-10  carbocyclyl, 3-14 membered heterocyclyl, C 6-14  aryl, and 5-14 membered heteroaryl, or two R bb  groups are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R dd  groups; wherein X −  is a counterion; 
     each instance of R cc  is, independently, selected from hydrogen, C 1-10  alkyl, C 1-10  perhaloalkyl, C 2-10  alkenyl, C 2-10  alkynyl, heteroC 1-10  alkyl, heteroC 2-10  alkenyl, heteroC 2-10  alkynyl, C 3-10  carbocyclyl, 3-14 membered heterocyclyl, C 6-14  aryl, and 5-14 membered heteroaryl, or two R cc  groups are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R dd  groups; 
     each instance of R dd  is, independently, selected from halogen, —CN, —NO 2 , —N 3 , —SO 2 H, —SO 3 H, —OH, —OR ee , —ON(R ff ) 2 , —N(R ff ) 2 , —N(R ff ) 3 +X − , —N(OR ee )R ff , —SH, —SR ee , —SSR ee , —C(═O)R ee , —CO 2 H, —CO 2 R ee , —OC(═O)R ee , —OCO 2 R ee , —C(═O)N(R ff ) 2 , —OC(═O)N(R ff ) 2 , —NR ff C(═O)R ee , —NR ff CO 2 R ee , —NR ff C(═O)N(R ff ) 2 , —C(═NR ff )OR ee , —OC(═NR ff )R ee , —OC(═NR ff )OR ee , —C(═NR ff )N(R ff ) 2 , —OC(═NR ff )N(R ff ) 2 , —NR ff C(═NR ff )N(R ff ) 2 , —NR ff SO 2 R ee , —SO 2 N(R ff ) 2 , —SO 2 R ee , —SO 2 OR ee , —OSO 2 R ee , —S(═O)R ee , —Si(R ee ) 3 , —OSi(R ee ) 3 , —C(═S)N(R ff ) 2 , —C(═O)SR ee , —C(═S)SR ee , —SC(═S)SR ee , —P(═O)(OR ee ) 2 , —P(═O)(R ee ) 2 , —OP(═O)(R ee ) 2 , —OP(═O)(OR ee ) 2 , C 1-6  alkyl, C 1-6  perhaloalkyl, C 2-6  alkenyl, C 2-6  alkynyl, heteroC 1-6 alkyl, heteroC 2-6 alkenyl, heteroC 2-6 alkynyl, C 3-10  carbocyclyl, 3-10 membered heterocyclyl, C 6-10  aryl, 5-10 membered heteroaryl, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R gg  groups, or two geminal R dd  substituents can be joined to form O or ═S; wherein X −  is a counterion; 
     each instance of R ee  is, independently, selected from C 1-6  alkyl, C 1-6  perhaloalkyl, C 2-6  alkenyl, C 2-6  alkynyl, heteroC 1-6  alkyl, heteroC 2-6 alkenyl, heteroC 2-6  alkynyl, C 3-10  carbocyclyl, C 6-10  aryl, 3-10 membered heterocyclyl, and 3-10 membered heteroaryl, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R gg  groups; 
     each instance of R ff  is, independently, selected from hydrogen, C 1-6  alkyl, C 1-6  perhaloalkyl, C 2-6  alkenyl, C 2-6  alkynyl, heteroC 1-6 alkyl, heteroC 2-6 alkenyl, heteroC 2-6 alkynyl, C 3-10  carbocyclyl, 3-10 membered heterocyclyl, C 6-10  aryl and 5-10 membered heteroaryl, or two R ff  groups are joined to form a 3-10 membered heterocyclyl or 5-10 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R gg  groups; and 
     each instance of R gg  is, independently, halogen, —CN, —NO 2 , —N 3 , —SO 2 H, —SO 3 H, —OH, —OC 1-6  alkyl, —ON(C 1-6  alkyl) 2 , —N(C 1-6  alkyl) 2 , —N(C 1-6  alkyl) 3 +X − , —NH(C 1-6  alkyl) 2 +X − , —NH 2 (C 1-6  alkyl)+X − , —NH 3 +X − , —N(OC 1-6  alkyl)(C 1-6  alkyl), —N(OH)(C 1-6  alkyl), —NH(OH), —SH, —SC 1-6  alkyl, —SS(C 1-6  alkyl), —C(═O)(C 1-6  alkyl), —CO 2 H, —CO 2 (C 1-6  alkyl), —OC(═O)(C 1-6  alkyl), —OCO 2 (C 1-6  alkyl), —C(═O)NH 2 , —C(═O)N(C 1-6  alkyl) 2 , —OC(═O)NH(C 1-6  alkyl), —NHC(═O)(C 1-6  alkyl), —N(C 1-6  alkyl)C(═O)(C 1-6  alkyl), —NHCO 2 (C 1-6  alkyl), —NHC(═O)N(C 1-6  alkyl) 2 , —NHC(═O)NH(C 1-6  alkyl), —NHC(═O)NH 2 , —C(═NH)O(C 1-6  alkyl), —OC(═NH)(C 1-6  alkyl), —OC(═NH)OC 1-6  alkyl, —C(═NH)N(C 1-6  alkyl) 2 , —C(═NH)NH(C 1-6  alkyl), —C(═NH)NH 2 , —OC(═NH)N(C 1-6  alkyl) 2 , —OC(NH)NH(C 1-6  alkyl), —OC(NH)NH 2 , —NHC(NH)N(C 1-6  alkyl) 2 , —NHC(═NH)NH 2 , —NHSO 2 (C 1-6  alkyl), —SO 2 N(C 1-6  alkyl) 2 , —SO 2 NH(C 1-6  alkyl), —SO 2 NH 2 , —SO 2 C 1-6  alkyl, —SO 2 OC 1-6  alkyl, —OSO 2 C 1-6  alkyl, —SOC 1-6  alkyl, —Si(C 1-6  alkyl) 3 , —OSi(C 1-6  alkyl) 3  —C(═S)N(C 1-6  alkyl) 2 , C(═S)NH(C 1-6  alkyl), C(═S)NH 2 , —C(═O)S(C 1-6  alkyl), —C(═S)SC 1-6  alkyl, —SC(═S)SC 1-6  alkyl, —P(═O)(OC 1-6  alkyl) 2 , —P(═O)(C 1-6  alkyl) 2 , —OP(═O)(C 1-6  alkyl) 2 , —OP(═O)(OC 1-6  alkyl) 2 , C 1-6  alkyl, C 1-6  perhaloalkyl, C 2-6  alkenyl, C 2-6  alkynyl, heteroC 1-6 alkyl, heteroC 2-6 alkenyl, heteroC 2-6 alkynyl, C 3-10  carbocyclyl, C 6-10  aryl, 3-10 membered heterocyclyl, 5-10 membered heteroaryl; or two geminal R gg  substituents can be joined to form ═O or ═S; wherein X −  is a counterion. Alternatively, two geminal hydrogens on a carbon atom are replaced with the group ═O, ═S, ═NN(R bb ) 2 , ═NNR bb C(═O)R aa , ═NNR bb C(═O)OR aa , ═NNR bb S(═O) 2 R aa , ═NR bb , or ═NOR cc ; wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R dd  groups; wherein X −  is a counterion; 
     wherein: 
     each instance of R aa  is, independently, selected from C 1-10  alkyl, C 1-10  perhaloalkyl, C 2-10  alkenyl, C 2-10  alkynyl, heteroC 1-10  alkyl, heteroC 2-10 alkenyl, heteroC 2-10 alkynyl, C 3-10  carbocyclyl, 3-14 membered heterocyclyl, C 6-14  aryl, and 5-14 membered heteroaryl, or two R aa  groups are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R dd  groups; 
     each instance of R bb  is, independently, selected from hydrogen, —OH, —OR aa , —N(R cc ) 2 , —CN, —C(═O)R aa , —C(═O)N(R cc ) 2 , —CO 2 R aa , —SO 2 R aa , —C(═NR cc )OR aa , —C(═NR cc )N(R cc ) 2 , —SO 2 N(R cc ) 2 , —SO 2 R cc  —SO 2 OR cc , —SOR aa , —C(═S)N(R cc ) 2 , —C(═O)SR cc , —C(═S)SR cc , —P(═O)(R aa ) 2 , —P(═O)(OR cc ) 2 , —P(═O)(N(R cc ) 2 ) 2 , C 1-10  alkyl, C 1-10  perhaloalkyl, C 2-10  alkenyl, C 2-10  alkynyl, heteroC 1-10 alkyl, heteroC 2-10 alkenyl, heteroC 2-10 alkynyl, C 3-10  carbocyclyl, 3-14 membered heterocyclyl, C 6-14  aryl, and 5-14 membered heteroaryl, or two Rb groups are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R dd  groups; wherein X −  is a counterion; 
     each instance of R cc  is, independently, selected from hydrogen, C 1-10  alkyl, C 1-10  perhaloalkyl, C 2-10  alkenyl, C 2-10  alkynyl, heteroC 1-10  alkyl, heteroC 2-10  alkenyl, heteroC 2-10  alkynyl, C 3-10  carbocyclyl, 3-14 membered heterocyclyl, C 6-14  aryl, and 5-14 membered heteroaryl, or two R cc  groups are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R dd  groups; 
     each instance of R dd  is, independently, selected from halogen, —CN, —NO 2 , —N 3 , —SO 2 H, —SO 3 H, —OH, —OR ee , —ON(R ff ) 2 , —N(R ff ) 2 , —N(R ff ) 3 +X − , —N(OR ee )R ff , —SH, —SR ee , —SSR ee , —C(═O)R ee , —CO 2 H, —CO 2 R ee , —OC(═O)R ee , —OCO 2 R ee , —C(═O)N(R ff ) 2 , —OC(═O)N(R ff ) 2 , —NR ff C(═O)R ee , —NR ff CO 2 R ee , —NR ff C(═O)N(R ff ) 2 , —C(═NR ff )OR ee , —OC(═NR ff )R ee , —OC(═NR ff )OR ee , —C(═NR ff )N(R ff ) 2 , —OC(═NR ff )N(R ff ) 2 , —NR ff C(═NR ff )N(R ff ) 2 , —NR ff SO 2 R ee , —SO 2 N(R ff ) 2 , —SO 2 R ee , —SO 2 OR ee , —OSO 2 R ee , —S(═O)R ee , —Si(R ee ) 3 , —OSi(R ee ) 3 , —C(═S)N(R ff ) 2 , —C(═O)SR ee , —C(═S)SR ee , —SC(═S)SR ee , —P(═O)(OR ee ) 2 , —P(═O)(R ee ) 2 , —OP(═O)(R ee ) 2 , —OP(═O)(OR ee ) 2 , C 1-6  alkyl, C 1-6  perhaloalkyl, C 2-6  alkenyl, C 2-6  alkynyl, heteroC 1-6 alkyl, heteroC 2-6 alkenyl, heteroC 2-6 alkynyl, C 3-10  carbocyclyl, 3-10 membered heterocyclyl, C 6-10  aryl, 5-10 membered heteroaryl, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R gg  groups, or two geminal R dd  substituents can be joined to form O or ═S; wherein X −  is a counterion; 
     each instance of R ee  is, independently, selected from C 1-6  alkyl, C 1-6  perhaloalkyl, C 2-6  alkenyl, C 2-6  alkynyl, heteroC 1-6  alkyl, heteroC 2-6 alkenyl, heteroC 2-6  alkynyl, C 3-10  carbocyclyl, C 6-10  aryl, 3-10 membered heterocyclyl, and 3-10 membered heteroaryl, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R gg  groups; 
     each instance of R ff  is, independently, selected from hydrogen, C 1-6  alkyl, C 1-6  perhaloalkyl, C 2-6  alkenyl, C 2-6  alkynyl, heteroC 1-6 alkyl, heteroC 2-6 alkenyl, heteroC 2-6 alkynyl, C 3-10  carbocyclyl, 3-10 membered heterocyclyl, C 6-10  aryl and 5-10 membered heteroaryl, or two R groups are joined to form a 3-10 membered heterocyclyl or 5-10 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R gg  groups; and 
     each instance of R gg  is, independently, halogen, —CN, —NO 2 , —N 3 , —SO 2 H, —SO 3 H, —OH, —OC 1-6  alkyl, —ON(C 1-6  alkyl) 2 , —N(C 1-6  alkyl) 2 , —N(C 1-6  alkyl) 3 +X − , —NH(C 1-6  alkyl) 2 +X − , —NH 2 (C 1-6  alkyl)+X − , —NH 3 +X − , —N(OC 1-6  alkyl)(C 1-6  alkyl), —N(OH)(C 1-6  alkyl), —NH(OH), —SH, —SC 1-6  alkyl, —SS(C 1-6  alkyl), —C(═O)(C 1-6  alkyl), —CO 2 H, —CO 2 (C 1-6  alkyl), —OC(═O)(C 1-6  alkyl), —CO 2 (C 1-6  alkyl), —C(═O)NH 2 , —C(═O)N(C 1-6  alkyl) 2 , —OC(═O)NH(C 1-6  alkyl), —NHC(═O)(C 1-6  alkyl), —N(C 1-6  alkyl)C(═O)(C 1-6  alkyl), —NHCO 2 (C 1-6  alkyl), —NHC(═O)N(C 1-6  alkyl) 2 , —NHC(═O)NH(C 1-6  alkyl), —NHC(═O)NH 2 , —C(═NH)O(C 1-6  alkyl), —OC(═NH)(C 1-6  alkyl), —OC(═NH)OC 1-6  alkyl, —C(═NH)N(C 1-6  alkyl) 2 , —C(═NH)NH(C 1-6  alkyl), —C(═NH)NH 2 , —OC(═NH)N(C 1-6  alkyl) 2 , —OC(NH)NH(C 1-6  alkyl), —OC(NH)NH 2 , —NHC(NH)N(C 1-6  alkyl) 2 , —NHC(═NH)NH 2 , —NHSO 2 (C 1-6  alkyl), —SO 2 N(C 1-6  alkyl) 2 , —SO 2 NH(C 1-6  alkyl), —SO 2 NH 2 , —SO 2 C 1-6  alkyl, —SO 2 OC 1-6  alkyl, —OSO 2 C 1-6  alkyl, —SOC 1-6  alkyl, —Si(C 1-6  alkyl) 3 , —OSi(C 1-6  alkyl) 3  —C(═S)N(C 1-6  alkyl) 2 , C(═S)NH(C 1-6  alkyl), C(═S)NH 2 , —C(═O)S(C 1-6  alkyl), —C(═S)SC 1-6  alkyl, —SC(═S)SC 1-6  alkyl, —P(═O)(OC 1-6  alkyl) 2 , —P(═O)(C 1-6  alkyl) 2 , —OP(═O)(C 1-6  alkyl) 2 , —OP(═O)(OC 1-6  alkyl) 2 , C 1-6  alkyl, C 1-6  perhaloalkyl, C 2-6  alkenyl, C 2-6  alkynyl, heteroC 1-6 alkyl, heteroC 2-6 alkenyl, heteroC 2-6 alkynyl, C 3-10  carbocyclyl, C 6-10  aryl, 3-10 membered heterocyclyl, 5-10 membered heteroaryl; or two geminal R 99  substituents can be joined to form ═O or ═S; wherein X −  is a counterion. 
     A “counterion” or “anionic counterion” is a negatively charged group associated with a positively charged group in order to maintain electronic neutrality. An anionic counterion may be monovalent (i.e., including one formal negative charge). An anionic counterion may also be multivalent (i.e., including more than one formal negative charge), such as divalent or trivalent. Exemplary counterions include halide ions (e.g., F − , Cl − , Br − , I − ), NO 3   − , ClO 4   − , OH − , H 2 PO 4   − , HCO 3   − , HSO 4   − , sulfonate ions (e.g., methansulfonate, trifluoromethanesulfonate, p-toluenesulfonate, benzenesulfonate, 10-camphor sulfonate, naphthalene-2-sulfonate, naphthalene-1-sulfonic acid-5-sulfonate, ethan-1-sulfonic acid-2-sulfonate, and the like), carboxylate ions (e.g., acetate, propanoate, benzoate, glycerate, lactate, tartrate, glycolate, gluconate, and the like), BF 4   − , PF 4   − , PF 6 , AsF 6   − , SbF 6   − , B[3,5-(CF 3 ) 2 C 6 H 3 ] 4 ] − , B(C 6 F 5 ) 4   − , BPh 4 , Al(OC(CF 3 ) 3 ) 4   − , and carborane anions (e.g., CB 11 H 12   −  or (HCB 11 Me 5 Br 6 ) − ). Exemplary counterions which may be multivalent include CO 3   2− , HPO 4   2− , PO 4   3− , B 4 O 7   2− , SO 4   2− , S 2 O 3   2− , carboxylate anions (e.g., tartrate, citrate, fumarate, maleate, malate, malonate, gluconate, succinate, glutarate, adipate, pimelate, suberate, azelate, sebacate, salicylate, phthalates, aspartate, glutamate, and the like), and carboranes. 
     The term “pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, Berge et al., describe pharmaceutically acceptable salts in detail in  J. Pharmaceutical Sciences,  1977, 66, 1-19, incorporated by reference. Pharmaceutically acceptable salts of the compounds disclosed in this application include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid, and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid, or malonic acid or by using other methods known in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N+(C 1-4  alkyl) 4  salts. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, lower alkyl sulfonate, and aryl sulfonate. 
     The term “solvate” refers to forms of a compound that are associated with a solvent, usually by a solvolysis reaction. This physical association may include hydrogen bonding. Conventional solvents include water, methanol, ethanol, acetic acid, DMSO, THF, diethyl ether, and the like. The compounds of Formula (1), (9), (10), and (11) may be prepared, e.g., in crystalline form, and may be solvated. Suitable solvates include pharmaceutically acceptable solvates and further include both stoichiometric solvates and non-stoichiometric solvates. In certain instances, the solvate will be capable of isolation, for example, when one or more solvent molecules are incorporated in the crystal lattice of a crystalline solid. “Solvate” encompasses both solution-phase and isolable solvates. Representative solvates include hydrates, ethanolates, and methanolates. 
     The term “hydrate” refers to a compound that is associated with water. Typically, the number of the water molecules contained in a hydrate of a compound is in a definite ratio to the number of the compound molecules in the hydrate. Therefore, a hydrate of a compound may be represented, for example, by the general formula R.x H 2 O, wherein R is the compound and wherein x is a number greater than 0. A given compound may form more than one type of hydrates, including, e.g., monohydrates (x is 1), lower hydrates (x is a number greater than 0 and smaller than 1, e.g., hemihydrates (R.0.5H 2 O)), and polyhydrates (x is a number greater than 1, e.g., dihydrates (R.2 H 2 O) and hexahydrates (R.6 H 2 O)). 
     The term “tautomers” refer to compounds that are interchangeable forms of a particular compound structure, and that vary in the displacement of hydrogen atoms and electrons. Thus, two structures may be in equilibrium through the movement of π electrons and an atom (usually H). For example, enols and ketones are tautomers because they are rapidly interconverted by treatment with either acid or base. Another example of tautomerism is the aci- and nitro-forms of phenylnitromethane, which are likewise formed by treatment with acid or base. Tautomeric forms may be relevant to the attainment of the optimal chemical reactivity and biological activity of a compound of interest. 
     It is also to be understood that compounds that have the same molecular formula but differ in the nature or sequence of bonding of their atoms or the arrangement of their atoms in space are termed “isomers.” Isomers that differ in the arrangement of their atoms in space are termed “stereoisomers.” 
     Stereoisomers that are not mirror images of one another are termed “diastereomers” and those that are non-superimposable mirror images of each other are termed “enantiomers.” When a compound has an asymmetric center, for example, it is bonded to four different groups, a pair of enantiomers is possible. An enantiomer can be characterized by the absolute configuration of its asymmetric center and described by the R- and S-sequencing rules of Cahn and Prelog. An enantiomer can also be characterized by the manner in which the molecule rotates the plane of polarized light, and designated as dextrorotatory or levorotatory (i.e., as (+) or (−)-isomers respectively). A chiral compound can exist as either an individual enantiomer or as a mixture of enantiomers. A mixture containing equal proportions of the enantiomers is called a “racemic mixture.” 
     The term “co-crystal” refers to a crystalline structure comprising at least two different components (e.g., a compound described in this application and an acid), wherein each of the components is independently an atom, ion, or molecule. In certain embodiments, none of the components is a solvent. In certain embodiments, at least one of the components is a solvent. A co-crystal of a compound and an acid is different from a salt formed from a compound and the acid. In the salt, a compound described in this application is complexed with the acid in a way that proton transfer (e.g., a complete proton transfer) from the acid to a compound described in this application easily occurs at room temperature. In the co-crystal, however, a compound described in this application is complexed with the acid in a way that proton transfer from the acid to a compound described in this application does not easily occur at room temperature. In certain embodiments, in the co-crystal, there is no proton transfer from the acid to a compound described in this application. In certain embodiments, in the co-crystal, there is partial proton transfer from the acid to a compound described in this application. Co-crystals may be useful to improve the properties (e.g., solubility, stability, and ease of formulation) of a compound described in this application. 
     The term “polymorphs” refers to a crystalline form of a compound (or a salt, hydrate, or solvate thereof) in a particular crystal packing arrangement. All polymorphs of the same compound have the same elemental composition. Different crystalline forms usually have different X-ray diffraction patterns, infrared spectra, melting points, density, hardness, crystal shape, optical and electrical properties, stability, and solubility. Recrystallization solvent, rate of crystallization, storage temperature, and other factors may cause one crystal form to dominate. Various polymorphs of a compound can be prepared by crystallization under different conditions. 
     The term “prodrug” refers to compounds, including derivatives of the compounds of Formula (X), (8), (9), (10), or (11), that have cleavable groups and become by solvolysis or under physiological conditions the compounds of Formula (X), (8), (9), (10), or (11) and that are pharmaceutically active in vivo. The prodrugs may have attributes such as, without limitation, solubility, bioavailability, tissue compatibility, or delayed release in a mammalian organism. Examples include, but are not limited to, derivatives of compounds described in this application, including derivatives formed from glycosylation of the compounds described in this application (e.g., glycoside derivatives), carrier-linked prodrugs (e.g., ester derivatives), bioprecursor prodrugs (a prodrug metabolized by molecular modification into the active compound), and the like. Non-limiting examples of glycoside derivatives are disclosed in and incorporated by reference from PCT Publication No. WO2018208875 and U.S. Patent Publication No. 2019/0078168. Non-limiting examples of ester derivatives are disclosed in and incorporated by reference from U.S. Patent Publication No. US2017/0362195. 
     Other derivatives of the compounds of this invention have activity in both their acid and acid derivative forms, but the acid sensitive form often offers advantages of solubility, bioavailability, tissue compatibility, or delayed release in a mammalian organism (see, Bundgard, H.,  Design of Prodrugs , pp. 7-9, 21-24, Elsevier, Amsterdam 1985). Prodrugs include acid derivatives well known to practitioners of the art, such as, for example, esters prepared by reaction of the parent acid with a suitable alcohol, or amides prepared by reaction of the parent acid compound with a substituted or unsubstituted amine, or acid anhydrides, or mixed anhydrides. Simple aliphatic or aromatic esters, amides, and anhydrides derived from acidic groups pendant on the compounds of this invention are particular prodrugs. In some cases it is desirable to prepare double ester type prodrugs such as (acyloxy)alkyl esters or ((alkoxycarbonyl)oxy)alkylesters. C 1 -C 8  alkyl, C 2 -C 8  alkenyl, C 2 -C 8  alkynyl, aryl, C 7 -C 12  substituted aryl, and C 7 -C 12  arylalkyl esters of the compounds of Formula (X), (8), (9), (10), or (11) may be preferred. 
     Cannabinoids 
     As used in this application, the term “cannabinoid” includes compounds of Formula (X): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt, co-crystal, tautomer, stereoisomer, solvate, hydrate, polymorph, isotopically enriched derivative, or prodrug thereof, wherein R1 is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; R2 and R6 are, independently, hydrogen or carboxyl; R3 and R5 are, independently, hydroxyl, halogen, or alkoxy; and R4 is a hydrogen or an optionally substituted prenyl moiety; or optionally R4 and R3 are taken together with their intervening atoms to form a cyclic moiety, or optionally R4 and R5 are taken together with their intervening atoms to form a cyclic moiety, or optionally both 1) R4 and R3 are taken together with their intervening atoms to form a cyclic moiety and 2) R4 and R5 are taken together with their intervening atoms to form a cyclic moiety. In certain embodiments, R4 and R3 are taken together with their intervening atoms to form a cyclic moiety. In certain embodiments, R4 and R5 are taken together with their intervening atoms to form a cyclic moiety. In certain embodiments, “cannabinoid” refers to a compound of Formula (X), or a pharmaceutically acceptable salt thereof. In certain embodiments, both 1) R4 and R3 are taken together with their intervening atoms to form a cyclic moiety and 2) R4 and R5 are taken together with their intervening atoms to form a cyclic moiety. 
     In some embodiments, cannabinoids may be synthesized via the following steps: a) one or more reactions to incorporate three additional ketone moieties onto an acyl-CoA scaffold, where the acyl moiety in the acyl-CoA scaffold comprises between four and fourteen carbons; b) a reaction cyclizing the product of step (a); and c) a reaction to incorporate a prenyl moiety to the product of step (b) or a derivative of the product of step (b). In some embodiments, non-limiting examples of the acyl-CoA scaffold described in step (a) include hexanoyl-CoA and butyryl-CoA. In some embodiments, non-limiting examples of the product of step (b) or a derivative of the product of step (b) include olivetolic acid divarinic acid, and sphaerophorolic acid. 
     In some embodiments, a cannabinoid compound of Formula (X) is of Formula (X-A), (X-B), or (X-C): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof;
 
wherein   is a double bond or a single bond, as valency permits;
 
     R is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; 
     R Z1  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; 
     R Z2  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; 
     or optionally, R Z1  and R Z2  are taken together with their intervening atoms to form an optionally substituted carbocyclic ring; 
     R 3A  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, or optionally substituted alkynyl; 
     R 3B  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, or optionally substituted alkynyl; 
     R Y  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, or optionally substituted alkynyl; 
     R Z  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, or optionally substituted alkynyl. 
     In certain embodiments, a cannabinoid compound is of Formula (X-A): 
     
       
         
         
             
             
         
       
     
     wherein   is a double bond, and each of R Z1  and R Z2  is hydrogen, one of R 3A  and R 3B  is optionally substituted C 2-6  alkenyl, and the other one of R 3A  and R 3B  is optionally substituted C 2-6  alkyl. In some embodiments, a cannabinoid compound of Formula (X) is of Formula (X-A), wherein each of R Z1  and R Z2  is hydrogen, one of R 3A  and R 3B  is a prenyl group, and the other one of R 3A  and R 3B  is optionally substituted methyl. 
     In certain embodiments, a cannabinoid compound of Formula (X) of Formula (X-A) is of Formula (11-z): 
     
       
         
         
             
             
         
       
     
     wherein   is a double bond or single bond, as valency permits; one of R 3A  and R 3B  is C 1-6  alkyl optionally substituted with alkenyl, and the other of R 3A  and R 3B  is optionally substituted C 1-6  alkyl. In certain embodiments, in a compound of Formula (11-z),   is a single bond; one of R 3A  and R 3B  is C 1-6  alkyl optionally substituted with prenyl; and the other of one of R 3A  and R 3B  is unsubstituted methyl; and R is as described in this application. In certain embodiments, in a compound of Formula (11-z),   is a single bond; one of R 3A  and R 3B  is 
     
       
         
         
             
             
         
       
     
     and the other of one of R 3A  and R 3B  is unsubstituted methyl; and R is as described in this application. In certain embodiments, a cannabinoid compound of Formula (11-z) is of Formula (11a): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a cannabinoid compound of Formula (X) of Formula (X-A) is of Formula (11a): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a cannabinoid compound of Formula (X-A) is of Formula (10-z): 
     
       
         
         
             
             
         
       
     
     wherein   is a double bond or single bond, as valency permits; R Y  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, or optionally substituted alkynyl; and each of R 3A  and R 3B  is independently optionally substituted C 1-6  alkyl. In certain embodiments, in a compound of Formula (10-z),   is a single bond; each of R 3A  and R 3B  is unsubstituted methyl, and R is as described in this application. In certain embodiments, a cannabinoid compound of Formula (10-z) is of Formula (10a): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     has a chiral atom labeled with * at carbon 10 and a chiral atom labeled with ** at carbon 6. In certain embodiments, in a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the R-configuration or S-configuration; and a chiral atom labeled with ** at carbon 6 is of the R-configuration. In certain embodiments, in a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the S-configuration; and a chiral atom labeled with ** at carbon 6 is of the R-configuration or S-configuration. In certain embodiments, in a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the R-configuration and a chiral atom labeled with ** at carbon 6 is of the R-configuration. In certain embodiments, a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, in a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the S-configuration and a chiral atom labeled with ** at carbon 6 is of the S-configuration. In certain embodiments, a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a cannabinoid compound is of Formula (X-B): 
     
       
         
         
             
             
         
       
     
     wherein   is a double bond; R Y  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, or optionally substituted alkynyl; and each of R 3A  and R 3B  is independently optionally substituted C 1-6  alkyl. In certain embodiments, in a compound of Formula (X-B), R Y  is optionally substituted C 1 -6 alkyl; one of R 3A  and R 3B  is 
     
       
         
         
             
             
         
       
     
     and the other one of R 3A  and R 3B  is unsubstituted methyl, and R is as described in this application. In certain embodiments, a compound of Formula (X-B) is of Formula (9a): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     has a chiral atom labeled with * at carbon 3 and a chiral atom labeled with ** at carbon 4. In certain embodiments, in a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the R-configuration or S-configuration; and a chiral atom labeled with ** at carbon 4 is of the R-configuration. In certain embodiments, in a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the S-configuration; and a chiral atom labeled with ** at carbon 4 is of the R-configuration or S-configuration. In certain embodiments, in a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the R-configuration and a chiral atom labeled with ** at carbon 4 is of the R-configuration. In certain embodiments, a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, in a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the S-configuration and a chiral atom labeled with ** at carbon 4 is of the S-configuration. In certain embodiments, a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a cannabinoid compound is of Formula (X-C): 
     
       
         
         
             
             
         
       
     
     wherein R Z  is optionally substituted alkyl or optionally substituted alkenyl. In certain embodiments, a compound of Formula (X-C) is of formula: 
     
       
         
         
             
             
         
       
     
     wherein a is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10. In certain embodiments, a is 1. In certain embodiments, a is 2. In certain embodiments, a is 3. In certain embodiments, a is 1, 2, or 3 for a compound of Formula (X-C). In certain embodiments, a cannabinoid compound is of Formula (X-C), and a is 1, 2, 3, 4, or 5. In certain embodiments, a compound of Formula (X-C) is of Formula (8a): 
     
       
         
         
             
             
         
       
     
     In some embodiments, cannabinoids of the present disclosure comprise cannabinoid receptor ligands. Cannabinoid receptors are a class of cell membrane receptors in the G protein-coupled receptor superfamily. Cannabinoid receptors include the CB 1  receptor and the CB 2  receptor. In some embodiments, cannabinoid receptors comprise GPR18, GPR55, and PPAR. (See Bram et al. “Activation of GPR18 by cannabinoid compounds: a tale of biased agonism”  Br J Pharmcol  v171 (16) (2014); Shi et al. “The novel cannabinoid receptor GPR55 mediates anxiolytic-like effects in the medial orbital cortex of mice with acute stress”  Molecular Brain  10, No. 38 (2017); and O&#39;Sullvan, Elizabeth. “An update on PPAR activation by cannabinoids”  Br J Pharmcol  v. 173(12) (2016)). 
     In some embodiments, cannabinoids comprise endocannabinoids, which are substances produced within the body, and phytocannabinoids, which are cannabinoids that are naturally produced by plants of genus  Cannabis . In some embodiments, phytocannabinoids comprise the acidic and decarboxylated acid forms of the naturally-occurring plant-derived cannabinoids, and their synthetic and biosynthetic equivalents. 
     Over 94 phytocannabinoids have been identified to date (Berman, Paula, et al. “A new ESI-LC/MS approach for comprehensive metabolic profiling of phytocannabinoids in  Cannabis.” Scientific reports  8.1 (2018): 14280; El-Alfy et al., 2010, “Antidepressant-like effect of delta-9-tetrahydrocannabinol and other cannabinoids isolated from  Cannabis sativa  L”, Pharmacology Biochemistry and Behavior 95 (4): 434-42; Rudolf Brenneisen, 2007, Chemistry and Analysis of Phytocannabinoids, Citti, Cinzia, et al. “A novel phytocannabinoid isolated from  Cannabis sativa  L. with an in vivo cannabimimetic activity higher than Δ9-tetrahydrocannabinol: Δ9-Tetrahydrocannabiphorol.” Sci Rep 9 (2019): 20335, each of which is incorporated by reference in this application in its entirety). In some embodiments, cannabinoids comprise Δ 9 -tetrahydrocannabinol (THC) type (e.g., (−)-trans-delta-9-tetrahydrocannabinol or dronabinol, (+)-trans-delta-9-tetrahydrocannabinol, (−)-cis-delta-9-tetrahydrocannabinol, or (+)-cis-delta-9-tetrahydrocannabinol), cannabidiol (CBD) type, cannabigerol (CBG) type, cannabichromene (CBC) type, cannabicyclol (CBL) type, cannabinodiol (CBND) type, or cannabitriol (CBT) type cannabinoids, or any combination thereof (see, e.g., R Pertwee, ed,  Handbook of Cannabis  (Oxford, UK: Oxford University Press, 2014)), which is incorporated by reference in this application in its entirety). A non-limiting list of cannabinoids comprises: cannabiorcol-C1 (CBNO), CBND-C1 (CBNDO), Δ 9 -trans-Tetrahydrocannabiorcolic acid-C1 (Δ 9 -THCO), Cannabidiorcol-C1 (CBDO), Cannabiorchromene-C1 (CBCO), (−)-Δ 8 -trans-(6aR,10aR)-Tetrahydrocannabiorcol-C1 (Δ 8 -THCO), Cannabiorcyclol C1 (CBLO), CBG-C1 (CBGO), Cannabinol-C2 (CBN-C2), CBND-C2, Δ 9 -THC-C2, CBD-C2, CBC-C2, Δ 8 -THC-C2, CBL-C2, Bisnor-cannabielsoin-C1 (CBEO), CBG-C2, Cannabivarin-C3 (CBNV), Cannabinodivarin-C3 (CBNDV), (−)-Δ 9 -trans-Tetrahydrocannabivarin-C3 (Δ 9 -THCV), (−)-Cannabidivarin-C3 (CBDV), (±)-Cannabichromevarin-C3 (CBCV), (−)-Δ 8 -trans-THC-C3 (Δ 8 -THCV), (±)-(1aS,3aR,8bR,8cR)-Cannabicyclovarin-C3 (CBLV), 2-Methyl-2-(4-methyl-2-pentenyl)-7-propyl-2H-1-benzopyran-5-ol, Δ 7 -tetrahydrocannabivarin-C3 (Δ 7 -THCV), CBE-C2, Cannabigerovarin-C3 (CBGV), Cannabitriol-C1 (CBTO), Cannabinol-C4 (CBN-C4), CBND-C4, (−)-Δ 9 -trans-Tetrahydrocannabinol-C4 (Δ 9 -THC-C4), Cannabidiol-C4 (CBD-C4), CBC-C4, (−)-trans-Δ 8 -THC-C4, CBL-C4, Cannabielsoin-C3 (CBEV), CBG-C4, CBT-C2, Cannabichromanone-C3, Cannabiglendol-C3 (OH-iso-HHCV-C3), Cannabioxepane-C5 (CBX), Dehydrocannabifuran-C5 (DCBF), Cannabinol-C5 (CBN), Cannabinodiol-C5 (CBND), (−)-Δ 9 -trans-Tetrahydrocannabinol-C5 (Δ 9 -THC), (−)-Δ 8 -trans-(6aR,10aR)-Tetrahydrocannabinol-C5 (Δ 8 -THC), (±)-Cannabichromene-C5 (CBC), (−)-Cannabidiol-C5 (CBD), (±)-(1aS,3aR,8bR,8cR)-CannabicyclolC5 (CBL), Cannabicitran-C5 (CBR), (−)-Δ 9 -(6aS,10aR-cis)-Tetrahydrocannabinol-C5 ((−)-cis-Δ 9 -THC), (−)-Δ 7 -trans-(1R,3R,6R)-Isotetrahydrocannabinol-C5 (trans-isoΔ 7 -THC), CBE-C4, Cannabigerol-C5 (CBG), Cannabitriol-C3 (CBTV), Cannabinol methyl ether-C5 (CBNM), CBNDM-C5, 8-OH—CBN-C5 (OH-CBN), OH-CBND-C5 (OH-CBND), 10-Oxo-Δ 6a(10a) -Tetrahydrocannabinol-C5 (OTHC), Cannabichromanone D-C5, Cannabicoumaronone-C5 (CBCON-C5), Cannabidiol monomethyl ether-C5 (CBDM), Δ 9 -THCM-C5, (±)-3″-hydroxy-Δ 4 ″-cannabichromene-C5, (5aS,6S,9R,9aR)-Cannabielsoin-C5 (CBE), 2-geranyl-5-hydroxy-3-n-pentyl-1,4-benzoquinone-C5, 5-geranyl olivetolic acid. 5-geranyl olivetolate, 8α-Hydroxy-Δ 9 -Tetrahydrocannabinol-C5 (8α-OH-Δ 9 -THC), 8β-Hydroxy-Δ 9 -Tetrahydrocannabinol-C5 (8β-OH-Δ 9 -THC), 10α-Hydroxy-Δ 8 -Tetrahydrocannabinol-C5 (10α-OH-Δ 8 -THC), 10β-Hydroxy-Δ 8 -Tetrahydrocannabinol-C5 (10β-OH-Δ 8 -THC), 10α-hydroxy-Δ 9,11 -hexahydrocannabinol-C5, 9β,10β-Epoxyhexahydrocannabinol-C5, OH-CBD-C5 (OH-CBD), Cannabigerol monomethyl ether-C5 (CBGM), Cannabichromanone-C5, CBT-C4, (±)-6,7-cis-epoxycannabigerol-C5, (±)-6,7-trans-epoxycannabigerol-C5, (−)-7-hydroxycannabichromane-C5, Cannabimovone-C5, (−)-trans-Cannabitriol-C5 ((−)-trans-CBT), (+)-trans-Cannabitriol-C5 ((+)-trans-CBT), (±)-cis-Cannabitriol-C5 ((±)-cis-CBT), (−)-trans-10-Ethoxy-9-hydroxy-Δ 6a(10a) -tetrahydrocannabivarin-C3 [(−)-trans-CBT-OEt], (−)-(6aR,9S,10S,10aR)-9,10-Dihydroxyhexahydrocannabinol-C5 [(−)-Cannabiripsol] (CBR), Cannabichromanone C-C5, (−)-6a,7,10a-Trihydroxy-Δ 9 -tetrahydrocannabinol-C5 [(−)-Cannabitetrol] (CBTT), Cannabichromanone B-C5, 8,9-Dihydroxy-Δ 6a(10a) -tetrahydrocannabinol-C5 (8,9-Di-OHCBT), (±)-4-acetoxycannabichromene-C5, 2-acetoxy-6-geranyl-3-n-pentyl-1,4-benzoquinone-C5, 11-Acetoxy-Δ9-TetrahydrocannabinolC5 (11-OAc-Δ9-THC), 5-acetyl-4-hydroxycannabigerol-C5, 4-acetoxy-2-geranyl-5-hydroxy-3-npentylphenol-C5, (−)-trans-10-Ethoxy-9-hydroxy-Δ 6a(10a) -tetrahydrocannabinol-C5 ((−)-trans-CBTOEt), sesquicannabigerol-C5 (SesquiCBG), carmagerol-C5, 4-terpenyl cannabinolate-C5, β-fenchyl-Δ 9 -tetrahydrocannabinolate-C5, α-fenchyl-Δ 9 -tetrahydrocannabinolate-C5, epi-bornyl-Δ 9 -tetrahydrocannabinolate-C5, bornyl-Δ 9 -tetrahydrocannabinolate-C5, α-terpenyl-Δ 9 -tetrahydrocannabinolate-C5, 4-terpenyl-Δ 9 -tetrahydrocannabinolate-C5, 6,6,9-trimethyl-3-pentyl-6H-dibenzo[b,d]pyran-1-ol, 3-(1,1-dimethylheptyl)-6,6a,7,8,10,10a-hexahydro-1-hydroxy-6,6-dimethyl-9H-dibenzo[1b,]pyran-9-one, (−)-(3S,4)-7-hydroxy-6-tetrahydrocannabinol-1,1-dimethylheptyl, (+)-(3S,4S)-7-hydroxy-Δ 6 -tetrahydrocannabinol-1,1-dimethylheptyl, 11-hydroxy-Δ 9 -tetrahydrocannabinol, and Δ 8 -tetrahydrocannabinol-11-oic acid)); certain piperidine analogs (e.g., (−)-(6S,6aR,9R,10aR)-5,6,6a,7,8,9,10,10a-octahydro-6-methyl-3-[(R)-1-methyl-4-phenylbutoxy]-1,9-phenanthridinediol 1-acetate)), certain aminoalkylindole analogs (e.g., (R)-(+)-[2,3-dihydro-5-methyl-3-(4-morpholinylmethyl)-pyrrolo[1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenyl-methanone), certain open pyran ring analogs (e.g., 2-[3-methy-6-(1-methylethenyl)-2-cyclohexen-1-yl]-5-pentyl-1,3-benzenediol and 4-(1,1-dimethylheptyl)-2,3′-dihydroxy-6′alpha-(3-hydroxypropyl)-1′,2′,3′,4′,5′,6′-hexahydrobiphenyl, tetrahydrocannabiphorol (THCP), cannabidiphorol (CBDP), CBGP, CBCP, their acidic forms, salts of the acidic forms, dimers of any combination of the above, trimers of any combination of the above, polymers of any combination of the above, or any combination thereof. 
     A cannabinoid described in this application can be a rare cannabinoid. For example, in some embodiments, a cannabinoid described in this application corresponds to a cannabinoid that is naturally produced in conventional  Cannabis  varieties at concentrations of less than 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, 0.6%, 0.5%, 0.25%, or 0.1% by dry weight of the female flower. In some embodiments, rare cannabinoids include CBGA, CBGVA, THCVA, CBDVA, CBCVA, and CBCA. In some embodiments, rare cannabinoids are cannabinoids that are not THCA, THC, CBDA or CBD. 
     A cannabinoid described in this application can also be a non-rare cannabinoid. 
     In some embodiments, the cannabinoid is selected from the cannabinoids listed in Table 1. 
     
       
         
           
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 Non-limiting examples of cannabinoids according to the present disclosure. 
               
               
                   
               
             
            
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Δ 9 -Tetrahydro- 
               
               
                 cannabinol 
               
               
                 Δ 9 -THC-C 5   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Δ 9 -Tetrahydro- 
               
               
                 cannabinol-C 4   
               
               
                 Δ 9 -THC-C 4   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Δ 9 -Tetrahydro- 
               
               
                 cannabivarin 
               
               
                 Δ 9 -THCV-C 3   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Δ 9 -Tetrahydro- 
               
               
                 cannbiorcol 
               
               
                 Δ 9 -THCO-C 1   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (−)-(6aS,10aR)-Δ 9 - 
               
               
                 Tetrahydro- 
               
               
                 cannabinol 
               
               
                 (−)-cis-Δ 9 -THC-C 5   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Δ 9 -Tetrahydro- 
               
               
                 cannabinolic acid A 
               
               
                 Δ 9 -THCA-C 5  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Δ 9 -Tetrahydro- 
               
               
                 cannabinolic acid B 
               
               
                 Δ 9 -THCA-C 5  B 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Δ 9 -Tetrahydro- 
               
               
                 cannabinolic acid-C 4   
               
               
                 A and/or B 
               
               
                 Δ 9 -THCA-C 4  A and/or B 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Δ 9 -Tetrahydro- 
               
               
                 cannabivarinic acid 
               
               
                 A 
               
               
                 Δ 9 -THCVA-C 3  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Δ 9 -Tetrahydro- 
               
               
                 cannabiorcolic acid 
               
               
                 A and/or B 
               
               
                 Δ 9 -THCOA-C 1  A 
               
               
                 and/or B 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (−)-Δ 8 -trans- 
               
               
                 (6aR,10aR)- 
               
               
                 Δ 8 -Tetrahydro- 
               
               
                 cannabinol 
               
               
                 Δ 8 -THC-C 5   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (−)-Δ 8 -trans- 
               
               
                 (6aR,10aR)- 
               
               
                 Tetrahydro- 
               
               
                 cannabinolic 
               
               
                 acid A 
               
               
                 Δ 8 -THCA-C 5  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (−)-Cannabidiol 
               
               
                 CBD-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabidiol 
               
               
                 momomethyl ether 
               
               
                 CBDM-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabidiol-C4 
               
               
                 CBD-C4 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabidiolic acid 
               
               
                 CBDA-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabidivarinic acid 
               
               
                 CBDVA-C3 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (−)-Cannabidivarin 
               
               
                 CBDV-C3 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabidiorcol 
               
               
                 CBD-C1 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabigerolic acid 
               
               
                 A 
               
               
                 (E)-CBGA-C 5  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabigerol 
               
               
                 (E)-CBG-C 5   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabigerol 
               
               
                 monomethyl ether 
               
               
                 (E)-CBGM-C 5  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabinerolic acid A 
               
               
                 (Z)-CBGA-C 5  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabigerovarin 
               
               
                 (E)-CBGV-C 3   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabigerol 
               
               
                 (E)-CBG-C 5   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabigerolic acid 
               
               
                 A 
               
               
                 (E)-CBGA-C 5  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabigerolic acid A 
               
               
                 monomethyl ether 
               
               
                 (E)-CBGAM-C 5  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabigerovarinic 
               
               
                 acid A 
               
               
                 (E)-CBGVA-C 3  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabinolic acid A 
               
               
                 CBNA-C5 A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabinol methyl 
               
               
                 ether 
               
               
                 CBNM-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabinol 
               
               
                 CBN-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabinol-C4 
               
               
                 CBN-C4 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabivarin 
               
               
                 CBN-C3 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabinol-C2 
               
               
                 CBN-C2 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabiorcol 
               
               
                 CBN-C1 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (±)- 
               
               
                 Cannabichromene 
               
               
                 CBC-C 5   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (±)-Cannabichromenic 
               
               
                 acid A 
               
               
                 CBCA-C 5  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (±)- 
               
               
                 Cannabivarichromene, 
               
               
                 (±)- 
               
               
                 Cannabichromevarin 
               
               
                 CBCV-C 3   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (±)-Cannabichro- 
               
               
                 mevarinic 
               
               
                 acid A 
               
               
                 CBCVA-C 3  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (±)- 
               
               
                 Cannabichromene 
               
               
                 CBC-C 5   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (±)- 
               
               
                 (1aS,3aR,8bR,8cR)- 
               
               
                 Cannabicyclol 
               
               
                 CBL-C 5   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (±)-(1aS,3aR,8bR,8cR)- 
               
               
                 Cannabicyclolic acid A 
               
               
                 CBLA-C 5  A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (±)-(1aS,3aR,8bR,8cR)- 
               
               
                 cannabicyclovarin 
               
               
                 CBLV-C 3   
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (−)-(9R,10R)-trans- 
               
               
                 10-O-Ethyl- 
               
               
                 cannabitriol 
               
               
                 (−)-trans-CBT-OEt- 
               
               
                 C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (±)-(9R,10R/9S,10S)- 
               
               
                 Cannabitriol-C3 
               
               
                 (±)-trans-CBT-C3 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (−)-(9R,10R)-trans- 
               
               
                 Cannabitriol 
               
               
                 (−)-trans-CBT-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (+)-(9S,10S)- 
               
               
                 Cannabitriol 
               
               
                 (+)-trans-CBT-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (±)-(9R,10S/9S,10R)- 
               
               
                 Cannabitriol 
               
               
                 (±)-cis-CBT-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (−)-6a,7,10a- 
               
               
                 Trihydroxy- 
               
               
                 Δ9- 
               
               
                 tetrahydrocannabinol 
               
               
                 (−)-Cannabitetrol 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 10-Oxo-Δ6a(10a)- 
               
               
                 tetrahydro- 
               
               
                 cannabinol 
               
               
                 OTHC 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 8,9-Dihydroxy- 
               
               
                 Δ6a(10a)- 
               
               
                 tetrahydro- 
               
               
                 cannabinol 
               
               
                 8,9-Di-OH-CBT-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabidiolic acid A 
               
               
                 cannabitriol ester 
               
               
                 CBDA-C5 9-OH-CBT-C5 
               
               
                 ester 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (−)-(6aR,9S,10S,10aR)- 
               
               
                 9,10-Dihydroxy- 
               
               
                 hexahydrocannabinol, 
               
               
                 Cannabiripsol 
               
               
                 Cannabiripsol-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (5aS,6S,9R,9aR)- 
               
               
                 Cannabielsoic acid B 
               
               
                 CBEA-C5 B 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (5aS,6S,9R,9aR)- 
               
               
                 C3-Cannabielsoic 
               
               
                 acid B 
               
               
                 CBEA-C3 B 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (5aS,6S,9R,9aR)- 
               
               
                 Cannabielsoin 
               
               
                 CBE-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (5aS,6S,9R,9aR)- 
               
               
                 C3-Cannabielsoin 
               
               
                 CBE-C3 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 (5aS,6S,9R,9aR)- 
               
               
                 Cannabielsoic acid A 
               
               
                 CBEA-C5 A 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabiglendol-C3 
               
               
                 OH-iso-HHCV-C3 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Dehydro- 
               
               
                 cannabifuran 
               
               
                 DCBF-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabifuran 
               
               
                 CBF-C5 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Cannabidiphorol 
               
               
                 (CBDP) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Tetrahydro- 
               
               
                 cannabiphorol 
               
               
                 (THCP) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
            
           
         
       
     
     Cannabinoids are often classified by “type,” i.e., by the topological arrangement of their prenyl moieties (See, for example, M. A. Elsohly and D. Slade, Life Sci., 2005, 78, 539-548; and L. O. Hanus et al. Nat. Prod. Rep., 2016, 33, 1357). Generally, each “type” of cannabinoid includes the variations possible for ring substitutions of the resorcinol moiety at the position meta to the two hydroxyl moieties. As used herein, a “CBG-type” cannabinoid is a 3-[(2E)-3,7-dimethylocta-2,6-dienyl]-2,4-dihydroxybenzoic acid optionally substituted at the 6 position of the benzoic acid moiety. As used herein, “CBC-type” cannabinoids refer to 5-hydroxy-2-methyl-2-(4-methylpent-3-enyl)-chromene-6-carboxylic acid optionally substituted at the 7 position of the chromene moiety. As used herein, a “THC-type” cannabinoid is a (6aR,10aR)-1-hydroxy-6,6,9-trimethyl-6a,7,8,10a-tetrahydrobenzo[c]chromene-2-carboxylic acid optionally substituted at the 3 position of the benzo[c]chromene moiety. As used herein, a “CBD-type” cannabinoid is a 2,4-dihydroxy-3-[(1R,6R)-3-methyl-6-prop-1-en-2-ylcyclohex-2-en-1-yl]-benzoic acid optionally substituted at the 6 position of the benzoic acid moiety. In some embodiments, the optional ring substitution for each “type” is an optionally substituted C1-C11 alkyl, an optionally substituted C1-C11 alkenyl, an optionally substituted C1-C11 alkynyl, or an optionally substituted C1-C11 aralkyl. 
     Biosynthesis of Cannabinoids and Cannabinoid Precursors 
     Aspects of the present disclosure provide tools, sequences, and methods for the biosynthetic production of cannabinoids in host cells. In some embodiments, the present disclosure teaches expression of enzymes that are capable of producing cannabinoids by biosynthesis. 
     As a non-limiting example, one or more of the enzymes depicted in  FIG.  2    may be used to produce a cannabinoid or cannabinoid precursor of interest.  FIG.  1    shows a cannabinoid biosynthesis pathway for the most abundant phytocannabinoids found in  Cannabis . See also, de Meijer et al. I, II, III, and IV (I: 2003,  Genetics,  163:335-346; II: 2005,  Euphytica,  145:189-198; III: 2009,  Euphytica,  165:293-311; and IV: 2009,  Euphytica,  168:95-112), and Carvalho et al. “Designing Microorganisms for Heterologous Biosynthesis of Cannabinoids” (2017)  FEMS Yeast Research  June 1; 17(4), each of which is incorporated by reference in this application in its entirety. 
     It should be appreciated that a precursor substrate for use in cannabinoid biosynthesis is generally selected based on the cannabinoid of interest. Non-limiting examples of cannabinoid precursors include compounds of Formulae (1)-(8) in  FIG.  2   . In some embodiments, polyketides, including compounds of Formula (5), could be prenylated. In certain embodiments, the precursor is a precursor compound shown in  FIG.  1 ,  2   , or  3 . Substrates in which R contains 1-40 carbon atoms are preferred. In some embodiments, substrates in which R contains 3-8 carbon atoms are most preferred. 
     As used in this application, a cannabinoid or a cannabinoid precursor may comprise an R group. See, e.g.,  FIG.  2   . In some embodiments, R may be a hydrogen. In certain embodiments, R is optionally substituted alkyl. In certain embodiments, R is optionally substituted C1-40 alkyl. In certain embodiments, R is optionally substituted C2-40 alkyl. In certain embodiments, R is optionally substituted C2-40 alkyl, which is straight chain or branched alkyl. In certain embodiments, R is optionally substituted C3-8 alkyl. In certain embodiments, R is optionally substituted C1-C40 alkyl, C1-C20 alkyl, C1-C10 alkyl, C1-C8 alkyl, C1-C5 alkyl, C3-C5 alkyl, C3 alkyl, or C5 alkyl. In certain embodiments, R is optionally substituted C1-C20 alkyl. In certain embodiments, R is optionally substituted C1-C10 alkyl. In certain embodiments, R is optionally substituted C1-C8 alkyl. In certain embodiments, R is optionally substituted C1-C5 alkyl. In certain embodiments, R is optionally substituted C1-C7 alkyl. In certain embodiments, R is optionally substituted C3-C5 alkyl. In certain embodiments, R is optionally substituted C3 alkyl. In certain embodiments, R is unsubstituted C3 alkyl. In certain embodiments, R is n-C3 alkyl. In certain embodiments, R is n-propyl. In certain embodiments, R is n-butyl. In certain embodiments, R is n-pentyl. In certain embodiments, R is n-hexyl. In certain embodiments, R is n-heptyl. In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is optionally substituted C4 alkyl. In certain embodiments, R is unsubstituted C4 alkyl. In certain embodiments, R is optionally substituted C5 alkyl. In certain embodiments, R is unsubstituted C5 alkyl. In certain embodiments, R is optionally substituted C6 alkyl. In certain embodiments, R is unsubstituted C6 alkyl. In certain embodiments, R is optionally substituted C7 alkyl. In certain embodiments, R is unsubstituted C7 alkyl. In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is optionally substituted n-propyl. In certain embodiments, R is n-propyl optionally substituted with optionally substituted aryl. In certain embodiments, R is n-propyl optionally substituted with optionally substituted phenyl. In certain embodiments, R is n-propyl substituted with unsubstituted phenyl. In certain embodiments, R is optionally substituted butyl. In certain embodiments, R is optionally substituted n-butyl. In certain embodiments, R is n-butyl optionally substituted with optionally substituted aryl. In certain embodiments, R is n-butyl optionally substituted with optionally substituted phenyl. In certain embodiments, R is n-butyl substituted with unsubstituted phenyl. In certain embodiments, R is optionally substituted pentyl. In certain embodiments, R is optionally substituted n-pentyl. In certain embodiments, R is n-pentyl optionally substituted with optionally substituted aryl. In certain embodiments, R is n-pentyl optionally substituted with optionally substituted phenyl. In certain embodiments, R is n-pentyl substituted with unsubstituted phenyl. In certain embodiments, R is optionally substituted hexyl. In certain embodiments, R is optionally substituted n-hexyl. In certain embodiments, R is optionally substituted n-heptyl. In certain embodiments, R is optionally substituted n-octyl. In certain embodiments, R is alkyl optionally substituted with aryl (e.g., phenyl). In certain embodiments, R is optionally substituted acyl (e.g., —C(═O)Me). 
     In certain embodiments, R is optionally substituted alkenyl (e.g., substituted or unsubstituted C 2-6  alkenyl). In certain embodiments, R is substituted or unsubstituted C 2-6  alkenyl. In certain embodiments, R is substituted or unsubstituted C 2-5  alkenyl. In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is optionally substituted alkynyl (e.g., substituted or unsubstituted C 2-6  alkynyl). In certain embodiments, R is substituted or unsubstituted C 2-6  alkynyl. In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is optionally substituted carbocyclyl. In certain embodiments, R is optionally substituted aryl (e.g., phenyl or napthyl). 
     The chain length of a precursor substrate can be from C1-C40. Those substrates can have any degree and any kind of branching or saturation or chain structure, including, without limitation, aliphatic, alicyclic, and aromatic. In addition, they may include any functional groups including hydroxy, halogens, carbohydrates, phosphates, methyl-containing or nitrogen-containing functional groups. 
     For example,  FIG.  3    shows a non-exclusive set of putative precursors for the cannabinoid pathway. Aliphatic carboxylic acids including four to eight total carbons (“C4”-“C8” in  FIG.  3   ) and up to 10-12 total carbons with either linear or branched chains may be used as precursors for the heterologous pathway. Non-limiting examples include methanoic acid, butyric acid, pentanoic acid, hexanoic acid, heptanoic acid, isovaleric acid, octanoic acid, and decanoic acid. Additional precursors may include ethanoic acid and propanoic acid. In some embodiments, in addition to acids, the ester, salt, and acid forms may all be used as substrates. Substrates may have any degree and any kind of branching, saturation, and chain structure, including, without limitation, aliphatic, alicyclic, and aromatic. In addition, they may include any functional modifications or combination of modifications including, without limitation, halogenation, hydroxylation, amination, acylation, alkylation, phenylation, and/or installation of pendant carbohydrates, phosphates, sulfates, heterocycles, or lipids, or any other functional groups. 
     Substrates for any of the enzymes disclosed in this application may be provided exogenously or may be produced endogenously by a host cell. In some embodiments, the cannabinoids are produced from a glucose substrate, so that compounds of Formula 1 shown in  FIG.  2    and CoA precursors are synthesized by the cell. In other embodiments, a precursor is fed into the reaction. In some embodiments, a precursor is a compound selected from Formulae 1-8 in  FIG.  2   . 
     Cannabinoids produced by methods disclosed in this application include rare cannabinoids. Due to the low concentrations at which cannabinoids, including rare cannabinoids occur in nature, producing industrially significant amounts of isolated or purified cannabinoids from the  Cannabis  plant may become prohibitive due to, e.g., the large volumes of  Cannabis  plants, and the large amounts of space, labor, time, and capital requirements to grow, harvest, and/or process the plant materials (see, for example, Crandall, K., 2016. A Chronic Problem: Taming Energy Costs and Impacts from Marijuana Cultivation. EQ Research; Mills, E., 2012. The carbon footprint of indoor  Cannabis  production. Energy Policy, 46, pp. 58-67; Jourabchi, M. and M. Lahet. 2014. Electrical Load Impacts of Indoor Commercial  Cannabis  Production. Presented to the Northwest Power and Conservation Council; O&#39;Hare, M., D. Sanchez, and P. Alstone. 2013. Environmental Risks and Opportunities in  Cannabis  Cultivation. Washington State Liquor and  Cannabis  Board; 2018. Comparing  Cannabis  Cultivation Energy Consumption. New Frontier Data; and Madhusoodanan, J., 2019. Can cannabis go green? Nature Outlook:  Cannabis ; all of which are incorporated by reference in this disclosure). The disclosure provided in this application represents a potentially efficient method for producing high yields of cannabinoids, including rare cannabinoids. The disclosure provided in this application also represents a potential method for addressing concerns related to agricultural practices and water usage associated with traditional methods of cannabinoid production (Dillis et al. “Water storage and irrigation practices for cannabis drive seasonal patterns of water extraction and use in Northern California.” Journal of Environmental Management 272 (2020): 110955, incorporated by reference in this disclosure). 
     Cannabinoids produced by the disclosed methods also include non-rare cannabinoids. Without being bound by a particular theory, the methods described in this application may be advantageous compared with traditional plant-based methods for producing non-rare cannabinoids. For example, methods provided in this application represent potentially efficient means for producing consistent and high yields of non-rare cannabinoids. With traditional methods of cannabinoid production, in which cannabinoids are harvested from plants, maintaining consistent and uniform conditions, including airflow, nutrients, lighting, temperature, and humidity, can be difficult. For example, with plant-based methods, there can be microclimates created by branching, which can lead to inconsistent yields and by-product formation. In some embodiments, the methods described in this application are more efficient at producing a cannabinoid of interest as compared to harvesting cannabinoids from plants. For example, with plant-based methods, seed-to-harvest can take up to half a year, while cutting-to-harvest usually takes about 4 months. Additional steps including drying, curing, and extraction are also usually needed with plant-based methods. In contrast, in some embodiments, the fermentation-based methods described in this application only take about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 days. In some embodiments, the fermentation-based methods described in this application only take about 3-5 days. In some embodiments, the fermentation-based methods described in this application only take about 5 days. In some embodiments, the methods provided in this application reduce the amount of security needed to comply with regulatory standards. For example, a smaller secured area may be needed to be monitored and secured to practice the methods described in this application as compared to the cultivation of plants. In some embodiments, the methods described in this application are advantageous over plant-sourced cannabinoids. 
     Terminal Synthases (TS) 
     A host cell described in this application may comprise a terminal synthase (TS). As used in this application, a “TS” refers to an enzyme that is capable of catalyzing oxidative cyclization of a prenyl moiety (e.g., terpene) to produce a ring-containing product (e.g., heterocyclic ring-containing product). In certain embodiments, a TS is capable of catalyzing oxidative cyclization of a prenyl moiety (e.g., terpene) to produce a carbocyclic-ring containing product (e.g., cannabinoid). In certain embodiments, a TS is capable of catalyzing oxidative cyclization of a prenyl moiety (e.g., terpene) to produce a heterocyclic-ring containing product (e.g., cannabinoid). In certain embodiments, a TS is capable of catalyzing oxidative cyclization of a prenyl moiety (e.g., terpene) to produce a cannabinoid. 
     TS enzymes are monomers that include FAD-binding and Berberine Bridge Enzyme (BBE) sequence motifs. 
     In some embodiments, the TS is an “ancestral” terminal synthase. Ancestral TSes can be generated from probabilistic models of mutations applied to terminal synthase phylogenes based on transcriptomic datasets. For example, Hochberg et al., describe a process for reconstructing ancestral proteins in  Annu. Rev. Biophys.  2017. 46:247-69, which is incorporated by reference in its entirety in this disclosure. 
     a. Substrates 
     A TS may be capable of using one or more substrates. In some instances, the location of the prenyl group and/or the R group differs between TS substrates. For example, a TS may be capable of using as a substrate one or more compounds of Formula (8w), Formula (8x), Formula (8′), Formula (8y), and/or Formula (8z): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein a is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10. 
     In certain embodiments, a compound of Formula (8′) is a compound of Formula (8): 
     
       
         
         
             
             
         
       
     
     In some embodiments, R is hydrogen, an optionally substituted C1-C11 alkyl, an optionally substituted C1-C11 alkenyl, an optionally substituted C1-C11 alkynyl, or an optionally substituted C1-C11 aralkyl. 
     In some embodiments, a TS catalyzes oxidative cyclization of the prenyl moiety (e.g., terpene) of a compound of Formula (8) described in this application and shown in  FIG.  2   . In certain embodiments, a compound of Formula (8) is a compound of Formula (8a): 
     
       
         
         
             
             
         
       
     
     In some embodiments, the production of a compound of Formula (11) from a particular substrate may be assessed relative to the production of a compound of Formula (11) from a control substrate. In some embodiments, the production of a compound of Formula (10) from a particular substrate may be assessed relative to the production of a compound of Formula (10) from a control substrate. In some embodiments, the production of a compound of Formula (9) from a particular substrate may be assessed relative to the production of a compound of Formula (9) from a control substrate. 
     b. Products 
     In some embodiments, TS enzymes catalyze the formation of CBD-type cannabinoids, THC-type cannabinoids and/or CBC-type cannabinoids from CBG-type cannabinoids. In embodiments where CBGA is the substrate, the TS enzymes CBDAS, THCAS and CBCAS would generally catalyze the formation of cannabidiolic acid (CBDA), A9-tetrahydrocannabinolic acid (THCA) and cannabichromenic acid (CBCA), respectively. However, in some embodiments, a TS can produce more than one different product depending on reaction conditions. Product promiscuity has been noted among the  Cannabis  terminal synthases (e.g., Zirpel et al., J. Biotechnol. 2018 Apr. 20; 272:40-7). Without wishing to be bound by any theory, it is believed that the reaction conditions affect the protonation state and orientation of the amino acids that form the substrate binding site of the TS enzymes, which may affect the docking of the substrate and/or products of these enzymes. For example, the pH of the reaction environment may cause a THCAS or a CBDAS to produce CBCA in greater proportions than THCA or CBDAS, respectively (see, for example, U.S. Pat. No. 9,359,625 to Winnicki and Donsky, incorporated by reference in its entirety). In some embodiments, a TS has a predetermined product specificity in intracellular conditions, such as cytosolic conditions or organelle conditions. By expressing a TS with a predetermined product specificity based on intracellular conditions, in vivo products produced by a cell expressing the TS may be more predictably produced. In some embodiments, a TS produces a desired product at a pH of 5.5. In some embodiments, a TS produces a desired product at a pH of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 or 14. In some embodiments, a TS produces a desired product at a pH that is between 4.5 and 8.0. In some embodiments, a TS produces a desired product at a pH that is between 5 and 6. In some embodiments, a TS produces a desired product at a pH that is around 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, or 8.0, including all values in between. In some embodiments, the product profile of a TS is dependent on the TS&#39;s signal peptide because the signal peptide targets the TS to a particular intracellular location having particular intracellular conditions (e.g. a particular organelle) that regulate the type of product produced by the TS. Exemplary signal peptides are discussed in further detail below. Differences in the intracellular conditions can affect the activity of the TS enzymes, for example, due to variations in pH and/or differences in the folding of TS enzymes due to the presence of chaperone proteins. 
     A TS may be capable of using one or more substrates described in this application to produce one or more products. Non-limiting example of TS products are shown in Table 1. In some instances, a TS is capable of using one substrate to produce 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 different products. In some embodiments, a TS is capable of using more than one substrate to produce 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 different products. 
     In some embodiments, a TS is capable of producing a compound of Formula (X-A) and/or a compound of Formula (X-B): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof;
 
wherein   is a double bond or a single bond, as valency permits;
 
     R is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; 
     R Z1  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; 
     R Z2  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; or optionally, R Z1  and R Z2  are taken together with their intervening atoms to form an optionally substituted carbocyclic ring; 
     R 3A  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, or optionally substituted alkynyl; 
     R 3B  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, or optionally substituted alkynyl; and/or 
     R Y  is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, or optionally substituted alkynyl. 
     In some embodiments, a compound of Formula (X-A) is: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a compound of Formula (10) 
     
       
         
         
             
             
         
       
     
     has a chiral atom labeled with * at carbon 10 and a chiral atom labeled with ** at carbon 6. In certain embodiments, in a compound of Formula (10) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the R-configuration or S-configuration; and a chiral atom labeled with ** at carbon 6 is of the R-configuration. In certain embodiments, in a compound of Formula (10) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the S-configuration; and a chiral atom labeled with ** at carbon 6 is of the R-configuration or S-configuration. In certain embodiments, in a compound of Formula (10) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the R-configuration and a chiral atom labeled with ** at carbon 6 is of the R-configuration. In certain embodiments, a compound of Formula (10) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, in a compound of Formula (10) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the S-configuration and a chiral atom labeled with ** at carbon 6 is of the S-configuration. In certain embodiments, a compound of Formula (10) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     has a chiral atom labeled with * at carbon 10 and a chiral atom labeled with ** at carbon 6. In certain embodiments, in a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the R-configuration or S-configuration; and a chiral atom labeled with ** at carbon 6 is of the R-configuration. In certain embodiments, in a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the S-configuration; and a chiral atom labeled with ** at carbon 6 is of the R-configuration or S-configuration. In certain embodiments, in a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the R-configuration and a chiral atom labeled with ** at carbon 6 is of the R-configuration. In certain embodiments, a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, in a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 10 is of the S-configuration and a chiral atom labeled with ** at carbon 6 is of the S-configuration. In certain embodiments, a compound of Formula (10a) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In some embodiments, a compound of Formula (X-A) is: 
     
       
         
         
             
             
         
       
     
     In some embodiments, a compound of Formula (X-A) is: 
     
       
         
         
             
             
         
       
     
     In some embodiments, a compound of Formula (X-B) is: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a compound of Formula (9) 
     
       
         
         
             
             
         
       
     
     has a chiral atom labeled with * at carbon 3 and a chiral atom labeled with ** at carbon 4. In certain embodiments, in a compound of Formula (9) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the R-configuration or S-configuration; and a chiral atom labeled with ** at carbon 4 is of the R-configuration. In certain embodiments, in a compound of Formula (9) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the S-configuration; and a chiral atom labeled with ** at carbon 4 is of the R-configuration or S-configuration. In certain embodiments, in a compound of Formula (9) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the R-configuration and a chiral atom labeled with ** at carbon 4 is of the R-configuration. In certain embodiments, a compound of Formula (9) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, in a compound of Formula (9) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the S-configuration and a chiral atom labeled with ** at carbon 4 is of the S-configuration. In certain embodiments, a compound of Formula (9) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a compound of Formula (9a) (CBDA) 
     
       
         
         
             
             
         
       
     
     has a chiral atom labeled with * at carbon 3 and a chiral atom labeled with ** at carbon 4. In certain embodiments, in a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the R-configuration or S-configuration; and a chiral atom labeled with ** at carbon 4 is of the R-configuration. In certain embodiments, in a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the S-configuration; and a chiral atom labeled with ** at carbon 4 is of the R-configuration or S-configuration. In certain embodiments, in a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the R-configuration and a chiral atom labeled with ** at carbon 4 is of the R-configuration. In certain embodiments, a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, in a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     the chiral atom labeled with * at carbon 3 is of the S-configuration and a chiral atom labeled with ** at carbon 4 is of the S-configuration. In certain embodiments, a compound of Formula (9a) 
     
       
         
         
             
             
         
       
     
     is of the formula: 
     
       
         
         
             
             
         
       
     
     In some embodiments, as shown in  FIG.  2   , a TS is capable of producing a cannabinoid from the product of a PT, including, without limitation, an enzyme capable of producing a compound of Formula (9), (10), or (11): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein R is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; produced from a compound of Formula (8′): 
     
       
         
         
             
             
         
       
     
     wherein a is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10; and R is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; or using any other substrate. In certain embodiments, a compound of Formula (8′) is a compound of Formula (8): 
     
       
         
         
             
             
         
       
     
     In certain embodiments, a compound of Formula (9), (10), or (11) is produced using a TS from a substrate compound of Formula (8′) (e.g., compound of Formula (8)), for example. Non-limiting examples of substrate compounds of Formula (8′) include but are not limited to cannabigerolic acid (CBGA), cannabigerovarinic acid (CBGVA), or cannabinerolic acid. In certain embodiments, at least one of the hydroxyl groups of the product compounds of Formula (9), (10), or (11) is further methylated. In certain embodiments, a compound of Formula (9) is methylated to form a compound of Formula (12): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. 
     Any of the enzymes, host cells, and methods described in this application may be used for the production of cannabinoids and cannabinoid precursors, such as those provided in Table 1. In general, the term “production” is used to refer to the generation of one or more products (e.g., products of interest and/or by-products/off-products), for example, from a particular substrate or reactant. The amount of production may be evaluated at any one or more steps of a pathway, such as a final product or an intermediate product, using metrics familiar to one of ordinary skill in the art. For example, the amount of production may be assessed for a single enzymatic reaction (e.g., conversion of a compound of Formula (8) to a compound of Formula (11) by a TS). Alternatively or in addition, the amount of production may be assessed for a series of enzymatic reactions (e.g., the biosynthetic pathway shown in  FIG.  1    and/or  FIG.  2   ). Production may be assessed by any metrics known in the art, for example, by assessing volumetric productivity, enzyme kinetics/reaction rate, specific productivity biomass-specific productivity, titer, yield, and total titer of one or more products (e.g., products of interest and/or by-products/off-products). 
     In some embodiments, the metric used to measure production may depend on whether a continuous process is being monitored (e.g., several cannabinoid biosynthesis steps are used in combination) or whether a particular end product is being measured. For example, in some embodiments, metrics used to monitor production by a continuous process may include volumetric productivity, enzyme kinetics and reaction rate. In some embodiments, metrics used to monitor production of a particular product may include specific productivity, biomass-specific productivity, titer, yield, and/or total titer of one or more products (e.g., products of interest and/or by-products/off-products). 
     Production of one or more products (e.g., products of interest and/or by-products/off-products) may be assessed indirectly, for example by determining the amount of a substrate remaining following termination of the reaction/fermentation. For example, for a TS that catalyzes the formation of products (e.g., a compound of Formula (11), including cannabichromenic acid (CBCA) (Formula (11a)) from a compound of Formula (8), including CBGA (Formula 8(a))), production of the products may be assessed by quantifying the compound of Formula (11) directly or by quantifying the amount of substrate remaining following the reaction (e.g., amount of the compound of Formula (8)). For a TS that catalyzes the formation of products (e.g., a compound of Formula (10), including tetrahydrocannabinolic acid (THCA) (Formula (10a)) from a compound of Formula (8), including CBGA (Formula 8(a))), production of the products may be assessed by quantifying the compound of Formula (10) directly or by quantifying the amount of substrate remaining following the reaction (e.g., amount of the compound of Formula (8)). For a TS that catalyzes the formation of products (e.g., a compound of Formula (9), including cannabidiolic acid (CBDA) (Formula (9a)) from a compound of Formula (8), including CBGA (Formula 8(a))), production of the products may be assessed by quantifying the compound of Formula (9) directly or by quantifying the amount of substrate remaining following the reaction (e.g., amount of the compound of Formula (8)). 
     In some embodiments, a TS that exhibits high production of by-products but low production of a desired product may still be used, for example if one or more amino acid substitutions, insertions, and/or deletions are introduced into the TS to shift production to the desired product, or if the TS can be expressed at locations where reaction conditions favor the production of the desired product. In some embodiments, the TS is a THCAS or has THCAS activity. Non-limiting by-products of a THCAS include compounds of Formulae (9) and (11) and a product resulting from the terpene of a compound of Formula (8) cyclizing with the other open —OH group (at carbon 1). In some embodiments, the TS is a CBDAS or has CBDAS activity. Non-limiting by-products of a CBDAS include compounds of Formulae (10) and (11) and a product resulting from the terpene of a compound of Formula (8) cyclizing with the other open —OH group (at carbon 1). In some embodiments, the TS is a CBCAS or has CBCAS activity. Non-limiting by-products of a CBCAS include compounds of Formula (9) or (10) and a product resulting from the terpene of a compound of Formula (8) cyclizing with the other open —OH group (at carbon 1). The carbons in a compound of Formula (8) may be numbered as follows: 
     
       
         
         
             
             
         
       
     
     See, e.g., Hanuš et al.,  Nat Prod Rep . (2016) November 23; 33(12):1357-1392. 
     In some embodiments, the production of a product (e.g., product of interest and/or by-product/off-product) by a particular TS may be assessed as relative production, for example relative to a control TS. In some embodiments, the production of a product by a particular host cell may be assessed relative to a control host cell. 
     In some embodiments, a TS or a host cell associated with the disclosure may be capable of producing a product at a higher titer or yield relative to a control. In some embodiments, a TS may be capable of producing a product at a faster rate (e.g., higher productivity) relative to a control. In some embodiments, a TS may have preferential binding and/or activity towards one substrate relative to another substrate. In some embodiments, a TS may preferentially produce one product relative to another product. 
     In some embodiments, a TS may produce at least 0.0001 μg/L, at least 0.001 μg/L, at least 0.01 μg/L, at least 0.02 μg/L, at least 0.03 μg/L, at least 0.04 μg/L, at least 0.05 μg/L, at least 0.06 μg/L, at least 0.07 μg/L, at least 0.08 μg/L, at least 0.09 μg/L, at least 0.1 μg/L, at least 0.11 μg/L, at least 0.12 μg/L, at least 0.13 μg/L, at least 0.14 μg/L, at least 0.15 μg/L, at least 0.16 μg/L, at least 0.17 μg/L, at least 0.18 μg/L, at least 0.19 μg/L, at least 0.2 μg/L, at least 0.21 μg/L, at least 0.22 μg/L, at least 0.23 μg/L, at least 0.24 μg/L, at least 0.25 μg/L, at least 0.26 μg/L, at least 0.27 μg/L, at least 0.28 μg/L, at least 0.29 μg/L, at least 0.3 μg/L, at least 0.31 μg/L, at least 0.32 μg/L, at least 0.33 μg/L, at least 0.34 μg/L, at least 0.35 μg/L, at least 0.36 μg/L, at least 0.37 μg/L, at least 0.38 μg/L, at least 0.39 μg/L, at least 0.4 μg/L, at least 0.41 μg/L, at least 0.42 μg/L, at least 0.43 μg/L, at least 0.44 μg/L, at least 0.45 μg/L, at least 0.46 μg/L, at least 0.47 μg/L, at least 0.48 μg/L, at least 0.49 μg/L, at least 0.5 μg/L, at least 0.51 μg/L, at least 0.52 μg/L, at least 0.53 μg/L, at least 0.54 μg/L, at least 0.55 μg/L, at least 0.56 μg/L, at least 0.57 μg/L, at least 0.58 μg/L, at least 0.59 μg/L, at least 0.6 μg/L, at least 0.61 μg/L, at least 0.62 μg/L, at least 0.63 μg/L, at least 0.64 μg/L, at least 0.65 μg/L, at least 0.66 μg/L, at least 0.67 μg/L, at least 0.68 μg/L, at least 0.69 μg/L, at least 0.7 μg/L, at least 0.71 μg/L, at least 0.72 μg/L, at least 0.73 μg/L, at least 0.74 μg/L, at least 0.75 μg/L, at least 0.76 μg/L, at least 0.77 μg/L, at least 0.78 μg/L, at least 0.79 μg/L, at least 0.8 μg/L, at least 0.81 μg/L, at least 0.82 μg/L, at least 0.83 μg/L, at least 0.84 μg/L, at least 0.85 μg/L, at least 0.86 μg/L, at least 0.87 μg/L, at least 0.88 μg/L, at least 0.89 μg/L, at least 0.9 μg/L, at least 0.91 μg/L, at least 0.92 μg/L, at least 0.93 μg/L, at least 0.94 μg/L, at least 0.95 μg/L, at least 0.96 μg/L, at least 0.97 μg/L, at least 0.98 μg/L, at least 0.99 μg/L, at least 1 μg/L, at least 1.1 μg/L, at least 1.2 μg/L, at least 1.3 μg/L, at least 1.4 μg/L, at least 1.5 μg/L, at least 1.6 μg/L, at least 1.7 μg/L, at least 1.8 μg/L, at least 1.9 μg/L, at least 2 μg/L, at least 2.1 μg/L, at least 2.2 μg/L, at least 2.3 μg/L, at least 2.4 μg/L, at least 2.5 μg/L, at least 2.6 μg/L, at least 2.7 μg/L, at least 2.8 μg/L, at least 2.9 μg/L, at least 3 μg/L, at least 3.1 μg/L, at least 3.2 μg/L, at least 3.3 μg/L, at least 3.4 μg/L, at least 3.5 μg/L, at least 3.6 μg/L, at least 3.7 μg/L, at least 3.8 μg/L, at least 3.9 μg/L, at least 4 μg/L, at least 4.1 μg/L, at least 4.2 μg/L, at least 4.3 μg/L, at least 4.4 μg/L, at least 4.5 μg/L, at least 4.6 μg/L, at least 4.7 μg/L, at least 4.8 μg/L, at least 4.9 μg/L, at least 5 μg/L, at least 5.1 μg/L, at least 5.2 μg/L, at least 5.3 μg/L, at least 5.4 μg/L, at least 5.5 μg/L, at least 5.6 μg/L, at least 5.7 μg/L, at least 5.8 μg/L, at least 5.9 μg/L, at least 6 μg/L, at least 6.1 μg/L, at least 6.2 μg/L, at least 6.3 μg/L, at least 6.4 μg/L, at least 6.5 μg/L, at least 6.6 μg/L, at least 6.7 μg/L, at least 6.8 μg/L, at least 6.9 μg/L, at least 7 μg/L, at least 7.1 μg/L, at least 7.2 μg/L, at least 7.3 μg/L, at least 7.4 μg/L, at least 7.5 μg/L, at least 7.6 μg/L, at least 7.7 μg/L, at least 7.8 μg/L, at least 7.9 μg/L, at least 8 μg/L, at least 8.1 μg/L, at least 8.2 μg/L, at least 8.3 μg/L, at least 8.4 μg/L, at least 8.5 μg/L, at least 8.6 μg/L, at least 8.7 μg/L, at least 8.8 μg/L, at least 8.9 μg/L, at least 9 μg/L, at least 9.1 μg/L, at least 9.2 μg/L, at least 9.3 μg/L, at least 9.4 μg/L, at least 9.5 μg/L, at least 9.6 μg/L, at least 9.7 μg/L, at least 9.8 μg/L, at least 9.9 μg/L, at least 10 μg/L, at least 10.1 μg/L, at least 10.2 μg/L, at least 10.3 μg/L, at least 10.4 μg/L, at least 10.5 μg/L, at least 10.6 μg/L, at least 10.7 μg/L, at least 10.8 μg/L, at least 10.9 μg/L, at least 11 μg/L, at least 11.1 μg/L, at least 11.2 μg/L, at least 11.3 μg/L, at least 11.4 μg/L, at least 11.5 μg/L, at least 11.6 μg/L, at least 11.7 μg/L, at least 11.8 μg/L, at least 11.9 μg/L, at least 12 μg/L, at least 12.1 μg/L, at least 12.2 μg/L, at least 12.3 μg/L, at least 12.4 μg/L, at least 12.5 μg/L, at least 12.6 μg/L, at least 12.7 μg/L, at least 12.8 μg/L, at least 12.9 μg/L, at least 13 μg/L, at least 13.1 μg/L, at least 13.2 μg/L, at least 13.3 μg/L, at least 13.4 μg/L, at least 13.5 μg/L, at least 13.6 μg/L, at least 13.7 μg/L, at least 13.8 μg/L, at least 13.9 μg/L, at least 14 μg/L, at least 14.1 μg/L, at least 14.2 μg/L, at least 14.3 μg/L, at least 14.4 μg/L, at least 14.5 μg/L, at least 14.6 μg/L, at least 14.7 μg/L, at least 14.8 μg/L, at least 14.9 μg/L, at least 15 μg/L, at least 15.1 μg/L, at least 15.2 μg/L, at least 15.3 μg/L, at least 15.4 μg/L, at least 15.5 μg/L, at least 15.6 μg/L, at least 15.7 μg/L, at least 15.8 μg/L, at least 15.9 μg/L, at least 16 μg/L, at least 16.1 μg/L, at least 16.2 μg/L, at least 16.3 μg/L, at least 16.4 μg/L, at least 16.5 μg/L, at least 16.6 μg/L, at least 16.7 μg/L, at least 16.8 μg/L, at least 16.9 μg/L, at least 17 μg/L, at least 17.1 μg/L, at least 17.2 μg/L, at least 17.3 μg/L, at least 17.4 μg/L, at least 17.5 μg/L, at least 17.6 μg/L, at least 17.7 μg/L, at least 17.8 μg/L, at least 17.9 μg/L, at least 18 μg/L, at least 18.1 μg/L, at least 18.2 μg/L, at least 18.3 μg/L, at least 18.4 μg/L, at least 18.5 μg/L, at least 18.6 μg/L, at least 18.7 μg/L, at least 18.8 μg/L, at least 18.9 μg/L, at least 19 μg/L, at least 19.1 μg/L, at least 19.2 μg/L, at least 19.3 μg/L, at least 19.4 μg/L, at least 19.5 μg/L, at least 19.6 μg/L, at least 19.7 μg/L, at least 19.8 μg/L, at least 19.9 μg/L, at least 20 μg/L, at least 25 μg/L, at least 30 μg/L, at least 35 μg/L, at least 40 μg/L, at least 45 μg/L, at least 50 μg/L, at least 55 μg/L, at least 60 μg/L, at least 65 μg/L, at least 70 μg/L, at least 75 μg/L, at least 80 μg/L, at least 85 μg/L, at least 90 μg/L, at least 95 μg/L, at least 100 μg/L, at least 105 μg/L, at least 110 μg/L, at least 115 μg/L, at least 120 μg/L, at least 125 μg/L, at least 130 μg/L, at least 135 μg/L, at least 140 μg/L, at least 145 μg/L, at least 150 μg/L, at least 155 μg/L, at least 160 μg/L, at least 165 μg/L, at least 170 μg/L, at least 175 μg/L, at least 180 μg/L, at least 185 μg/L, at least 190 μg/L, at least 195 μg/L, at least 200 μg/L, at least 205 μg/L, at least 210 μg/L, at least 215 μg/L, at least 220 μg/L, at least 225 μg/L, at least 230 μg/L, at least 235 μg/L, at least 240 μg/L, at least 245 μg/L, at least 250 μg/L, at least 255 μg/L, at least 260 μg/L, at least 265 μg/L, at least 270 μg/L, at least 275 μg/L, at least 280 μg/L, at least 285 μg/L, at least 290 μg/L, at least 295 μg/L, at least 300 μg/L, at least 305 μg/L, at least 310 μg/L, at least 315 μg/L, at least 320 μg/L, at least 325 μg/L, at least 330 μg/L, at least 335 μg/L, at least 340 μg/L, at least 345 μg/L, at least 350 μg/L, at least 355 μg/L, at least 360 μg/L, at least 365 μg/L, at least 370 μg/L, at least 375 μg/L, at least 380 μg/L, at least 385 μg/L, at least 390 μg/L, at least 395 μg/L, at least 400 μg/L, at least 405 μg/L, at least 410 μg/L, at least 415 μg/L, at least 420 μg/L, at least 425 μg/L, at least 430 μg/L, at least 435 μg/L, at least 440 μg/L, at least 445 μg/L, at least 450 μg/L, at least 455 μg/L, at least 460 μg/L, at least 465 μg/L, at least 470 μg/L, at least 475 μg/L, at least 480 μg/L, at least 485 μg/L, at least 490 μg/L, at least 495 μg/L, at least 500 μg/L, at least 600 μg/L, at least 700 μg/L, at least 800 μg/L, at least 900 μg/L, at least 1,000 μg/L, at least 2,000 μg/L, at least 3,000 μg/L, at least 4,000 μg/L, at least 5,000 μg/L, at least 6,000 μg/L, at least 7,000 μg/L, at least 8,000 μg/L, at least 9,000 μg/L, at least 10,000 μg/L, at least 11,000 μg/L, at least 12,000 μg/L, at least 13,000 μg/L, at least 14,000 μg/L, at least 15,000 μg/L, at least 16,000 μg/L, at least 17,000 μg/L, at least 18,000 μg/L, at least 19,000 μg/L, at least 20,000 μg/L, at least 21,000 μg/L, at least 22,000 μg/L, at least 23,000 μg/L, at least 24,000 μg/L, at least 25,000 μg/L, at least 26,000 μg/L, at least 27,000 μg/L, at least 28,000 μg/L, at least 29,000 μg/L, at least 30,000 μg/L, at least 31,000 μg/L, at least 32,000 μg/L, at least 33,000 μg/L, at least 34,000 μg/L, at least 35,000 μg/L, at least 36,000 μg/L, at least 37,000 μg/L, at least 38,000 μg/L, at least 39,000 μg/L, at least 40,000 μg/L, at least 41,000 μg/L, at least 42,000 μg/L, at least 43,000 μg/L, at least 44,000 μg/L, at least 45,000 μg/L, at least 46,000 μg/L, at least 47,000 μg/L, at least 48,000 μg/L, at least 49,000 μg/L, at least 50,000 μg/L, at least 51,000 μg/L, at least 52,000 μg/L, at least 53,000 μg/L, at least 54,000 μg/L, at least 55,000 μg/L, at least 56,000 μg/L, at least 57,000 μg/L, at least 58,000 μg/L, at least 59,000 μg/L, at least 60,000 μg/L, at least 61,000 μg/L, at least 62,000 μg/L, at least 63,000 μg/L, at least 64,000 μg/L, at least 65,000 μg/L, at least 66,000 μg/L, at least 67,000 μg/L, at least 68,000 μg/L, at least 69,000 μg/L, at least 70,000 μg/L, at least 71,000 μg/L, at least 72,000 μg/L, at least 73,000 μg/L, at least 74,000 μg/L, at least 75,000 μg/L, at least 76,000 μg/L, at least 77,000 μg/L, at least 78,000 μg/L, at least 79,000 μg/L, at least 80,000 μg/L, at least 81,000 μg/L, at least 82,000 μg/L, at least 83,000 μg/L, at least 84,000 μg/L, at least 85,000 μg/L, at least 86,000 μg/L, at least 87,000 μg/L, at least 88,000 μg/L, at least 89,000 μg/L, at least 90,000 μg/L, at least 91,000 μg/L, at least 92,000 μg/L, at least 93,000 μg/L, at least 94,000 μg/L, at least 95,000 μg/L, at least 96,000 μg/L, at least 97,000 μg/L, at least 98,000 μg/L, at least 99,000 μg/L, at least 100,000 μg/L, at least 105,000 μg/L, at least 110,000 μg/L, at least 115,000 μg/L, at least 120,000 μg/L, at least 125,000 μg/L, at least 130,000 μg/L, at least 135,000 μg/L, at least 140,000 μg/L, at least 145,000 μg/L, at least 150,000 μg/L, at least 155,000 μg/L, at least 160,000 μg/L, at least 165,000 μg/L, at least 170,000 μg/L, at least 175,000 μg/L, at least 180,000 μg/L, at least 185,000 μg/L, at least 190,000 μg/L, at least 195,000 μg/L, at least 200,000 μg/L, at least 205,000 μg/L, at least 210,000 μg/L, at least 215,000 μg/L, at least 220,000 μg/L, at least 225,000 μg/L, at least 230,000 μg/L, at least 235,000 μg/L, at least 240,000 μg/L, at least 245,000 μg/L, at least 250,000 μg/L, at least 255,000 μg/L, at least 260,000 μg/L, at least 265,000 μg/L, at least 270,000 μg/L, at least 275,000 μg/L, at least 280,000 μg/L, at least 285,000 μg/L, at least 290,000 μg/L, at least 295,000 μg/L, at least 300,000 μg/L, at least 305,000 μg/L, at least 310,000 μg/L, at least 315,000 μg/L, at least 320,000 μg/L, at least 325,000 μg/L, at least 330,000 μg/L, at least 335,000 μg/L, at least 340,000 μg/L, at least 345,000 μg/L, at least 350,000 μg/L, at least 355,000 μg/L, at least 360,000 μg/L, at least 365,000 μg/L, at least 370,000 μg/L, at least 375,000 μg/L, at least 380,000 μg/L, at least 385,000 μg/L, at least 390,000 μg/L, at least 395,000 μg/L, at least 400,000 μg/L, at least 405,000 μg/L, at least 410,000 μg/L, at least 415,000 μg/L, at least 420,000 μg/L, at least 425,000 μg/L, at least 430,000 μg/L, at least 435,000 μg/L, at least 440,000 μg/L, at least 445,000 μg/L, at least 450,000 μg/L, at least 455,000 μg/L, at least 460,000 μg/L, at least 465,000 μg/L, at least 470,000 μg/L, at least 475,000 μg/L, at least 480,000 μg/L, at least 485,000 μg/L, at least 490,000 μg/L, at least 495,000 μg/L, at least 500,000 μg/L, at least 600,000 μg/L, at least 700,000 μg/L, at least 800,000 μg/L, at least 900,000 μg/L, or at least 1,000,000 μg/L, including all values in between, of a product described herein. In some embodiments, a product is a compound of Formula (11) (e.g., a compound of Formula (11a)). In some embodiments, a product is CBCA and/or CBCVA. In some embodiments, a product is a compound of Formula (9) (e.g., the compound of Formula (9a)). In some embodiments, a product is a compound of Formula (10) (e.g., the compound of Formula (10a)). 
     In some embodiments, a TS or a host cell associated with the disclosure may be capable of producing more of an amount of one or more products than produced by a control (e.g., a positive control). In some embodiments, a TS or a host cell associated with the disclosure may be capable of producing at least 0.05% (e.g., at least 0.075%, at least 0.1%, at least 0.5%, at least 0.75%, at least 1%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 300%, at least 400%, at least 500%, at least 600%, at least 700%, at least 800%, at least 900%, or at least 1,000%) of the amount of one or more products produced by a control (e.g., such as a positive control). In some embodiments, a product is CBCA and/or CBCVA. In some embodiments, a TS or a host cell associated with the disclosure may be capable of producing at least 0.05% (e.g., at least 0.075%, at least 0.1%, at least 0.5%, at least 0.75%, at least 1%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 300%, at least 400%, at least 500%, at least 600%, at least 700%, at least 800%, at least 900%, or at least 1,000%) more of one or more products produced by a control (e.g., such as a positive control). In some embodiments, a product is a compound of Formula (11) (e.g., the compound of Formula (11a)). In some embodiments, a product is CBCA and/or CBCVA. In some embodiments, a product is a compound of Formula (9) (e.g., the compound of Formula (9a)). In some embodiments, a product is a compound of Formula (10) (e.g., the compound of Formula (10a)). 
     In some embodiments, a TS or a host cell associated with the disclosure may be capable of producing at least 0.05% (e.g., at least 0.075%, at least 0.1%, at least 0.5%, at least 0.75%, at least 1%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 300%, at least 400%, at least 500%, at least 600%, at least 700%, at least 800%, at least 900%, or at least 1,000%) of the titer or yield of one or more products produced by a control (e.g., such as a positive control). In some embodiments, a product is CBCA and/or CBCVA. In some embodiments, a TS or a host cell associated with the disclosure may be capable of producing at least 0.05% (e.g., at least 0.075%, at least 0.1%, at least 0.5%, at least 0.75%, at least 1%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 300%, at least 400%, at least 500%, at least 600%, at least 700%, at least 800%, at least 900%, or at least 1,000%) higher titer or yield of one or more products as compared to a control. In some embodiments, a product is a compound of Formula (11) (e.g., the compound of Formula (11a)). In some embodiments, a product is CBCA and/or CBCVA. In some embodiments, a product is a compound of Formula (9) (e.g., the compound of Formula (9a)). In some embodiments, a product is a compound of Formula (10) (e.g., the compound of Formula (10a)). 
     In some embodiments, a TS or host cell associated with the disclosure may be capable of producing one or more products at a rate that is at least 0.05% (e.g., at least 0.075%, at least 0.1%, at least 0.5%, at least 0.75%, at least 1%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 300%, at least 400%, at least 500%, at least 600%, at least 700%, at least 800%, at least 900%, or at least 1,000%) the rate of a control (e.g., such as a positive control). In some embodiments, a product is CBCA and/or CBCVA. In some embodiments, a TS may be capable of producing one or more products at a rate that is at least 1% (e.g., at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 300%, at least 400%, at least 500%, at least 600%, at least 700%, at least 800%, at least 900%, or at least 1,000%) faster relative to a control (e.g., such as a positive control). In some embodiments, a product is a compound of Formula (11) (e.g., a compound of Formula (11a)). In some embodiments, a product is CBCA and/or CBCVA. In some embodiments, a product is a compound of Formula (9) (e.g., the compound of Formula (9a)). In some embodiments, a product is a compound of Formula (10) (e.g., the compound of Formula (10a)). 
     In some embodiments, a TS or host cell associated with the disclosure may be capable of producing less of an amount of one or more products than produced by a control (e.g., a positive control). In some embodiments, a TS or host cell associated with the disclosure may be capable of producing at least 0.05% (e.g., at least 0.075%, at least 0.1% at least 0.5%, at least 0.75%, at least 1%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 300%, at least 400%, at least 500%, at least 600%, at least 700%, at least 800%, at least 900%, or at least 1,000%) less of one or more products relative to a control (e.g., such as a positive control). In some embodiments, a product is a compound of Formula (11) (e.g., the compound of Formula (11a)). In some embodiments, a product is CBCA and/or CBCVA. In some embodiments, a product is a compound of Formula (9) (e.g., the compound of Formula (9a)). In some embodiments, a product is a compound of Formula (10) (e.g., the compound of Formula (10a)). 
     In some embodiments, a TS or host cell associated with the disclosure may be capable of producing at least 0.05% (e.g., at least 0.075%, at least 0.1%, at least 0.5%, at least 0.75%, at least 1%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 300%, at least 400%, at least 500%, at least 600%, at least 700%, at least 800%, at least 900%, or at least 1,000%) lower titer or yield of one or more products relative to a control (e.g., such as a positive control). In some embodiments, a product is a compound of Formula (11) (e.g., the compound of Formula (11a)). In some embodiments, a product is CBCA and/or CBCVA. In some embodiments, a product is a compound of Formula (9) (e.g., the compound of Formula (9a)). In some embodiments, a product is a compound of Formula (10) (e.g., the compound of Formula (10a)). 
     In some embodiments, a TS or host cell associated with the disclosure may be capable of producing one or more products at a rate that is at least 0.5% (e.g., at least 0.075%, at least 0.1%, at least 0.5%, at least 0.75%, at least 1%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100%, at least 125%, at least 150%, at least 175%, at least 200%, at least 300%, at least 400%, at least 500%, at least 600%, at least 700%, at least 800%, at least 900%, or at least 1,000%) slower relative to a control (e.g., such as a positive control). In some embodiments, a product is a compound of Formula (11) (e.g., the compound of Formula (11a)). In some embodiments, a product is CBCA and/or CBCVA. In some embodiments, a product is a compound of Formula (9) (e.g., the compound of Formula (9a)). In some embodiments, a product is a compound of Formula (10) (e.g., the compound of Formula (10a)). 
     In some embodiments of methods described herein involving comparison of an experimental TS to a control, the control is a wild-type reference TS. In some embodiments, the control is a wild-type  C. sativa  THCAS (e.g., comprising SEQ ID NO: 21). In some embodiments, the control is a wild-type  C. sativa  THCAS (e.g., comprising SEQ ID NO: 21) that also exhibits CBCAS activity in addition to THCAS activity. In some embodiments, the control TS is identical to an experimental TS except for the presence of one or more amino acid substitutions, insertions, or deletions within the experimental TS. 
     In some embodiments of methods described herein involving comparison of an experimental host cell to a control host cell, the control host cell is a host cell that does not comprise a heterologous polynucleotide encoding a TS. In some embodiments, a control host cell is a wild-type cell. In some embodiments, a control host cell is a host cell that comprises a heterologous polynucleotide encoding a wild-type  C. sativa  THCAS. In some embodiments, the control is a wild-type  C. sativa  THCAS that also exhibits CBCAS activity in addition to THCAS activity. In  Cannabis , the wild-type CsTHCAS is secreted into glandular trichomes. However, as described in further detail below, it may be desirable to control the localization of a cannabinoid produced by the recombinant host cell, for example to a particular cellular compartment and/or the cellular secretory pathway. Accordingly, in some embodiments, the control is a wild-type  C. sativa  THCAS, that also exhibits CBCAS activity, in which the native signal sequence has been removed (e.g., as set forth in SEQ ID NO: 21) and, optionally, replaced with one or more heterologous signal sequences. In some embodiments, a control host cell is a host cell that comprises a heterologous polynucleotide comprising SEQ ID NO: 22. In some embodiments, a control host cell is genetically identical to an experimental host cell except for the the presence of one or more amino acid substitutions, insertions, or deletions within a TS that is heterologously expressed in the experimental host cell. 
     In some embodiments, a TS is capable of producing a mixture of products. For example, the mixture may comprise one or more compounds of Formula (11). In some embodiments, the mixture comprises a compound of Formula (9), Formula (10), and/or Formula (11). In some embodiments, at least approximately 50-100%, at least approximately 50-60%, at least approximately 60-70%, at least approximately 70-80%, at least approximately 80-90%, at least approximately 90-100%, of compounds within the product mixture are compounds of Formula (11a). In some embodiments, from about 50-100%, at least approximately 50%, at least approximately 60%, at least approximately 70%, at least approximately 80%, or at least approximately 90%, of compounds within the product mixture are CBCA. In some embodiments, from about 50-100%, at least approximately 50%, at least approximately 60%, at least approximately 70%, at least approximately 80%, or at least approximately 90%, of compounds within the product mixture are CBCVA. 
     In some embodiments, a TS is capable of producing at least 1.1 times, 1.2 times, 1.3 times, 1.4 times, 1.5 times, 1.6 times, 1.7 times, 1.8 times, 1.9 times, 2 times, 2.1 times, 2.2 times, 2.3 times, 2.4 times, 2.5 times, 2.6 times, 2.7 times, 2.8 times, 2.9 times, 3 times, 3.1 times, 3.2 times, 3.3 times, 3.4 times, 3.5 times, 3.6 times, 3.7 times, 3.8 times, 3.9 times, 4 times, 5 times, 6 times, 8 times, 9 times, 10 times, 20 times, 30 times, 40 times, 50 times, 60 times, 70 times, 80 times, 90 times, 100 times, 200 times, 300 times, 400 times, 500 times, 600 times, 700 times, 800 times or 1,000 times more of a compound of Formula (11a) than another compound of Formula (11), a compound of Formula (10a), a compound of Formula (9a), or any combination thereof. In some embodiments, a TS is capable of producing at least 1.1 times, 1.2 times, 1.3 times, 1.4 times, 1.5 times, 1.6 times, 1.7 times, 1.8 times, 1.9 times, 2 times, 2.1 times, 2.2 times, 2.3 times, 2.4 times, 2.5 times, 2.6 times, 2.7 times, 2.8 times, 2.9 times, 3 times, 3.1 times, 3.2 times, 3.3 times, 3.4 times, 3.5 times, 3.6 times, 3.7 times, 3.8 times, 3.9 times, 4 times, 5 times, 6 times, 8 times, 9 times, 10 times, 20 times, 30 times, 40 times, 50 times, 60 times, 70 times, 80 times, 90 times, 100 times, 200 times, 300 times, 400 times, 500 times, 600 times, 700 times, 800 times or 1,000 times less of a compound of Formula (11a) than another compound of Formula (11), a compound of Formula (10a), a compound of Formula (9a), or any combination thereof. 
     In some embodiments, at least approximately 50-100%, at least approximately 50-60%, at least approximately 60-70%, at least approximately 70-80%, at least approximately 80-90%, at least approximately 90-100%, of compounds within the product mixture are compounds of Formula (9a). In some embodiments, a TS is capable of producing at least 1.1 times, 1.2 times, 1.3 times, 1.4 times, 1.5 times, 1.6 times, 1.7 times, 1.8 times, 1.9 times, 2 times, 2.1 times, 2.2 times, 2.3 times, 2.4 times, 2.5 times, 2.6 times, 2.7 times, 2.8 times, 2.9 times, 3 times, 3.1 times, 3.2 times, 3.3 times, 3.4 times, 3.5 times, 3.6 times, 3.7 times, 3.8 times, 3.9 times, 4 times, 5 times, 6 times, 8 times, 9 times, 10 times, 20 times, 30 times, 40 times, 50 times, 60 times, 70 times, 80 times, 90 times, 100 times, 200 times, 300 times, 400 times, 500 times, 600 times, 700 times, 800 times or 1,000 times more of a compound of Formula (9a) than another compound of Formula (9), a compound of Formula (10a), a compound of Formula (11a), or any combination thereof. In some embodiments, a TS is capable of producing at least 1.1 times, 1.2 times, 1.3 times, 1.4 times, 1.5 times, 1.6 times, 1.7 times, 1.8 times, 1.9 times, 2 times, 2.1 times, 2.2 times, 2.3 times, 2.4 times, 2.5 times, 2.6 times, 2.7 times, 2.8 times, 2.9 times, 3 times, 3.1 times, 3.2 times, 3.3 times, 3.4 times, 3.5 times, 3.6 times, 3.7 times, 3.8 times, 3.9 times, 4 times, 5 times, 6 times, 8 times, 9 times, 10 times, 20 times, 30 times, 40 times, 50 times, 60 times, 70 times, 80 times, 90 times, 100 times, 200 times, 300 times, 400 times, 500 times, 600 times, 700 times, 800 times or 1,000 times less of a compound of Formula (9a) than another compound of Formula (9), a compound of Formula (10a), a compound of Formula (11a), or any combination thereof. 
     In some embodiments, at least approximately 50-100%, at least approximately 50-60%, at least approximately 60-70%, at least approximately 70-80%, at least approximately 80-90%, at least approximately 90-100%, of compounds within the product mixture are compounds of Formula (10a). In some embodiments, a TS is capable of producing at least 1.1 times, 1.2 times, 1.3 times, 1.4 times, 1.5 times, 1.6 times, 1.7 times, 1.8 times, 1.9 times, 2 times, 2.1 times, 2.2 times, 2.3 times, 2.4 times, 2.5 times, 2.6 times, 2.7 times, 2.8 times, 2.9 times, 3 times, 3.1 times, 3.2 times, 3.3 times, 3.4 times, 3.5 times, 3.6 times, 3.7 times, 3.8 times, 3.9 times, 4 times, 5 times, 6 times, 8 times, 9 times, 10 times, 20 times, 30 times, 40 times, 50 times, 60 times, 70 times, 80 times, 90 times, 100 times, 200 times, 300 times, 400 times, 500 times, 600 times, 700 times, 800 times or 1,000 times more of a compound of Formula (10a) than another compound of Formula (10), a compound of Formula (9a), a compound of Formula (11a), or any combination thereof. In some embodiments, a TS is capable of producing at least 1.1 times, 1.2 times, 1.3 times, 1.4 times, 1.5 times, 1.6 times, 1.7 times, 1.8 times, 1.9 times, 2 times, 2.1 times, 2.2 times, 2.3 times, 2.4 times, 2.5 times, 2.6 times, 2.7 times, 2.8 times, 2.9 times, 3 times, 3.1 times, 3.2 times, 3.3 times, 3.4 times, 3.5 times, 3.6 times, 3.7 times, 3.8 times, 3.9 times, 4 times, 5 times, 6 times, 8 times, 9 times, 10 times, 20 times, 30 times, 40 times, 50 times, 60 times, 70 times, 80 times, 90 times, 100 times, 200 times, 300 times, 400 times, 500 times, 600 times, 700 times, 800 times or 1,000 times less of a compound of Formula (10a) than another compound of Formula (10), a compound of Formula (9a), a compound of Formula (11a), or any combination thereof. 
     c. Signal Peptides 
     Any of the enzymes described in this application, including TSs, may comprise a signal peptide. Signal peptides, also referred to as “signal sequences,” generally comprise approximately 15-30 amino acids and are involved in regulating trafficking of a newly translated protein to a particular cellular compartment and/or the cellular secretory pathway. 
     In some instances, a signal peptide promotes localization of an enzyme of interest. A non-limiting example of a signal peptide that promotes localization of an enzyme of interest in intracellular spaces is the MFalpha2 signal peptide. See, e.g., the signal sequence from UniProtKB—U3N2MO (residues 1-19) and Singh et al.,  Nucleic Acids Res . (1983) June 25; 11(12): 4049-4063. In other instances, a signal peptide is capable of preventing a protein from being secreted from the endoplasmic reticulum (ER) and/or is capable of facilitating the return of such a protein if it is inadvertently exported. Such a signal peptide may be referred to as an “ER retentional signal.” A non-limiting example of a signal peptide that is capable of preventing a protein from being secreted from the ER and/or is capable of facilitating the return of such a protein if it is inadvertently exported is an HDEL signal peptide. See, e.g., Pelham et al.,  EMBO J  (1988)7:1757-1762. 
     Non-limiting examples of signal peptides include those listed in Table 2 below. As one of ordinary skill in the art would appreciate, other signal peptides known in the art would also be compatible with aspects of the disclosure. A signal peptide may be located N-terminal or C-terminal relative to a sequence encoding an enzyme of interest. A sequence encoding an enzyme of interest may be linked to two or more signal peptides. In some embodiments, an enzyme of interest may be linked to one or more signal peptides at the N-terminus and one or more signal peptides at the C-terminus. For example, in some embodiments, the MFalpha2 signal peptide may be located N-terminal to a sequence encoding an enzyme of interest and/or the HDEL signal peptide may be located C-terminal to a sequence encoding an enzyme of interest. In other embodiments, the HDEL signal peptide may be located N-terminal to a sequence encoding an enzyme of interest and/or the MFalpha2 signal peptide may be located C-terminal to a sequence encoding an enzyme of interest. 
     Without wishing to be bound by any theory, it is believed that an enzyme, such as a TS enzyme, linked to the MFalpha2 signal peptide and/or the HDEL signal peptide will be localized to intracellular locations associated with the secretory pathway, such as the ER and/or the Golgi apparatus. One or more of the conditions of the secretory pathway are believed to contribute to improved activity of TS enzymes derived from  C. sativa . For example, the ER and Golgi apparatus are oxidative environments, which may assist in the formation of disulphide bridges. Without wishing to be bound by any theory, signal peptides and the resulting intracellular localization of proteins containing the signal peptides may differentially impact the stability and/or half-life of proteins. 
     In some embodiments, a signal peptide comprises a nucleic acid or protein sequence that is at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or is 100% identical, including all values in between, to one or more of SEQ ID NOs: 3, 4, 16-19, 31, or 32. 
     In some embodiments, a signal peptide comprises a sequence that differs by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18 amino acids from any of SEQ ID NOs: 3, 4, 16, or 31. In some embodiments, a signal peptide comprises no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18 amino acid substitutions, insertions, additions, or deletions relative to the sequence of SEQ ID NOs: 3, 4, 16, or 31. In some embodiments, a signal peptide comprises SEQ ID NO: 16 or a sequence that has no more than 2 amino acid substitutions, insertions, additions, or deletions relative to the sequence of SEQ ID NO: 16. In some embodiments, a signal peptide comprises a protein sequence that differs by no more than 1, 2 or 3 amino acids from SEQ ID NO: 17. In some embodiments, a signal peptide comprises SEQ ID NO: 17 or a sequence that has no more than one amino acid substitution, insertion, addition, or deletion relative to the sequence of SEQ ID NO: 17. 
     A signal peptide that is located at the N-terminus of a sequence encoding an enzyme of interest may comprise a methionine at the N-terminus of the signal peptide. In some embodiments, a methionine is added to a signal peptide if the signal peptide will be located at the N-terminus of a sequence encoding an enzyme of interest. In some embodiments, a signal peptide that is normally associated with an enzyme of interest (e.g., a naturally occurring signal peptide that is present in a naturally occurring enzyme of interest) may be removed or replaced with one or more different signal peptides that are suitable for targeting the enzyme to a particular cellular compartment in a host cell of interest. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Non-limiting examples of signal peptides 
               
            
           
           
               
               
               
            
               
                   
                   
                 Non-limiting example of corresponding nucleic acid 
               
               
                 Name 
                 Amino acid sequence 
                 sequence 
               
               
                   
               
               
                   C .  sativa   
                 NCSAFSFWFVCKIIFFFLSFNI 
                 aattgctcagcattttccttttggtttgtttgcaaaataatatttttctttct 
               
               
                 THCAS 
                 QISIA (SEQ ID NO: 4) 
                 ctcattcaatatccaaatttcaata (SEQ ID NO: 3) 
               
               
                 native signal 
                   
                   
               
               
                 peptide 
                   
                   
               
               
                   
               
               
                 MFalpha2 
                 KFISTFLTFILAAVSVTA (SEQ 
                 aagtttatcagtaccttcttgacctttatcttggccgctgtctccgtaaccgc 
               
               
                   
                 ID NO: 16) 
                 t (SEQ ID NO: 18) 
               
               
                   
               
               
                 HDEL 
                 HDEL (SEQ ID NO: 17) 
                 catgatgaatta (SEQ ID NO: 19) 
               
               
                   
               
               
                   C .  sativa   
                 NCSTFSFWFVCKIIFFFLSFNIQ 
                 aattgctcaacattctccttttggtttgtttgcaaaataatatttttctttct 
               
               
                 CBCAS 
                 ISIA (SEQ ID NO: 31) 
                 ctcattcaatatccaa atttcaatagct (SEQ ID NO: 32) 
               
               
                 native signal 
                   
                   
               
               
                 peptide 
               
               
                   
               
            
           
         
       
     
     In some embodiments, a TS is a tetrahydrocannabinolic acid synthase (THCAS), a cannabidiolic acid synthase (CBDAS), and/or a cannabichromenic acid synthase (CBCAS). As one of ordinary skill in the art would appreciate a TS could be obtained from any source, including naturally occurring sources and synthetic sources (e.g., a non-naturally occurring TS). 
     Tetrahydrocannabinolic Acid Synthase (THCAS) 
     A host cell described in this application may comprise a TS that is a tetrahydrocannabinolic acid synthase (THCAS). As used in this application “tetrahydrocannabinolic acid synthase (THCAS)” or “Δ 1 -tetrahydrocannabinolic acid (THCA) synthase” refers to an enzyme that is capable of catalyzing oxidative cyclization of a prenyl moiety (e.g., terpene) of a compound of Formula (8) to produce a ring-containing product (e.g., heterocyclic ring-containing product, carbocyclic-ring containing product) of Formula (10). In certain embodiments, a THCAS refers to an enzyme that is capable of producing Δ9-tetrahydrocannabinolic acid (Δ9-THCA, THCA, Δ9-Tetrahydro-cannabivarinic acid A (Δ9-THCVA-C3 A), THCVA, THCPA, or a compound of Formula 10(a), from a compound of Formula (8). In certain embodiments, a THCAS is capable of producing Δ 9 -tetrahydrocannabinolic acid (Δ 9 -THCA, THCA, or a compound of Formula 10(a)). In certain embodiments, a THCAS is capable of producing Δ9-tetrahydrocannabivarinic acid (Δ9-THCVA, THCVA, or a compound of Formula 10 where R is n-propyl). 
     In some embodiments, a THCAS may catalyze the oxidative cyclization of substrates, such as 3-prenyl-2,4-dihydroxy-6-alkylbenzoic acids. In some embodiments, a THCAS may use cannabigerolic acid (CBGA) as a substrate. In some embodiments, the THCAS produces Δ9-THCA from CBGA. In some embodiments, a THCAS may catalyze the oxidative cyclization of cannabigerovarinic acid (CBGVA). In some embodiments, a THCAS exhibits specificity for CBGA substrates as compared to other substrates. In some embodiments, a THCAS may use a compound of Formula (8) of  FIG.  2    where R is C4 alkyl (e.g., n-butyl) or R is C7 alkyl (e.g., n-heptyl) as a substrate. In some embodiments, a THCAS may use a compound of Formula (8) where R is C4 alkyl (e.g., n-butyl) as a substrate. In some embodiments, a THCAS may use a compound of Formula (8) of  FIG.  2    where R is C7 alkyl (e.g., n-heptyl) as a substrate. In some embodiments, the THCAS exhibits specificity for substrates that can result in THCP as a product. 
     In some embodiments, a THCAS is from  C. sativa. C. sativa  THCAS performs the oxidative cyclization of the geranyl moiety of Cannabigerolic Acid (CBGA) ( FIG.  4    Structure 8a) to form Tetrahydrocannabinolic Acid ( FIG.  4    Structure 10a) using covalently bound flavin adenine dinucleotide (FAD) as a cofactor and molecular oxygen as the final electron acceptor. THCAS was first discovered and characterized by Taura et al. (JACS. 1995) following extraction of the enzyme from the leaf buds of  C. sativa  and confirmation of its THCA synthase activity in vitro upon the addition of CBGA as a substrate. A crystal structure of the enzyme published by Shoyama et al. ( J Mol Biol.  2012 Oct. 12; 423(1):96-105) revealed that the enzyme covalently binds to a molecule of the cofactor FAD. See also, e.g., Sirikantarams et al.,  J. Biol. Chem.  2004 Sep. 17; 279(38):39767-39774. There are several THCAS isozymes in  C. sativa.    
     In some embodiments, a  C. sativa  THCAS (Uniprot KB Accession No.: I1V0C5) comprises the amino acid sequence shown below, in which the signal peptide is underlined and bolded: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 20) 
               
               
                 M   NCSAFSFWFVCKIIFFFLSFNIQISIA   NPQENFLKCFSEYIPNNPANP 
               
               
                   
               
               
                 KFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASIL 
               
               
                   
               
               
                 CSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 
               
               
                   
               
               
                 WVEAGATLGEVYYWINEKNENFSFPGGYCPTVGVGGHFSGGGYGALMRN 
               
               
                   
               
               
                 YGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWK 
               
               
                   
               
               
                 IKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFI 
               
               
                   
               
               
                 TKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCK 
               
               
                   
               
               
                 EFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKPI 
               
               
                   
               
               
                 PETAMVKILEKLYEEDVGVGMYVLYPYGGIMEEISESAIPFPHRAGIMY 
               
               
                   
               
               
                 ELWYTASWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLG 
               
               
                   
               
               
                 KTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPP 
               
               
                   
               
               
                 LPPHHH. 
               
            
           
         
       
     
     In some embodiments, a THCAS comprises the sequence shown below: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 21) 
               
               
                 NPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDT 
               
               
                   
               
               
                 TPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGMSYISQVP 
               
               
                   
               
               
                 FVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENFSFPGGY 
               
               
                   
               
               
                 CPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGE 
               
               
                   
               
               
                 DLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVKLF 
               
               
                   
               
               
                 NKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVD 
               
               
                   
               
               
                 SLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILL 
               
               
                   
               
               
                 DRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGVGMYVLYPYG 
               
               
                   
               
               
                 GIMEEISESAIPFPHRAGIMYELWYTASWEKQEDNEKHINWVRSVYNFT 
               
               
                   
               
               
                 TPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRL 
               
               
                   
               
               
                 VKVKTKADPNNFFRNEQSIPPLPPHHH. 
               
            
           
         
       
     
     A non-limiting example of a nucleotide sequence encoding SEQ ID NO: 21 is: 
     
       
         
           
               
               
            
               
                 (SEQ ID NO: 22) 
                   
               
               
                 aacccgcaagaaaactttctaaaatgcttttctgaatacattcctaacaaccctgccaacccgaagtttatctacacacaacacgatcaat 
                   
               
               
                   
               
               
                 tgtatatgagcgtgttgaatagtacaatacagaacctgaggtttacatccgacacaacgccgaaaccgctagtgatcgtcacaccctcca 
               
               
                   
               
               
                 acgtaagccacattcaggcaagcattttatgcagcaagaaagtcggactgcagataaggacgaggtccggaggacacgacgccgaa 
               
               
                   
               
               
                 gggatgagctatatctcccaggtaccttttgtggtggtagacttgagaaatatgcactctatcaagatagacgttcactcccaaaccgctt 
               
               
                   
               
               
                 gggttgaggcgggagccacccttggtgaggtctactactggatcaacgaaaagaatgaaaattttagctttcctgggggatattgccca 
               
               
                   
               
               
                 actgtaggtgttggcggccacttctcaggaggcggttatggggccttgatgcgtaactacggacttgcggccgacaacattatagacg 
               
               
                   
               
               
                 cacatctagtgaatgtagacggcaaagttttagacaggaagagcatgggtgaggatcttttttgggcaattagaggcggagggggaga 
               
               
                   
               
               
                 aaattttggaattatcgctgcttggaaaattaagctagttgcggtaccgagcaaaagcactatattctctgtaaaaaagaacatggagata 
               
               
                   
               
               
                 catggtttggtgaagctttttaataagtggcaaaacatcgcgtacaagtacgacaaagatctggttctgatgacgcattttataacgaaaa 
               
               
                   
               
               
                 atatcaccgacaaccacggaaaaaacaaaaccacagtacatggctacttctctagtatatttcatgggggagtcgattctctggttgattt 
               
               
                   
               
               
                 aatgaacaaatcattcccagagttgggtataaagaagacagactgtaaggagttctcttggattgacacaactatattctattcaggcgta 
               
               
                   
               
               
                 gtcaactttaacacggcgaatttcaaaaaagagatccttctggacagatccgcaggtaagaaaactgcgttctctatcaaattggactatg 
               
               
                   
               
               
                 tgaagaagcctattcccgaaaccgcgatggtcaagatacttgagaaattatacgaggaagatgtgggagttggaatgtacgtactttatc 
               
               
                   
               
               
                 cctatggtgggataatggaagaaatcagcgagagcgccattccatttccccatcgtgccggcatcatgtacgagctgtggtatactgcg 
               
               
                   
               
               
                 agttgggagaagcaagaagacaacgaaaagcacattaactgggtcagatcagtttacaatttcaccaccccatacgtgtcccagaatc 
               
               
                   
               
               
                 cgcgtctggcttacttgaactaccgtgatcttgacctgggtaaaacgaacccggagtcacccaacaattacactcaagctagaatctgg 
               
               
                   
               
               
                 ggagagaaatactttgggaagaacttcaacaggttagtaaaggttaaaaccaaggcagatccaaacaacttttttagaaatgaacaatc 
               
               
                   
               
               
                 cattcccccgctacccccgcaccatcac. 
               
            
           
         
       
     
     In some embodiments, a THCAS comprises the amino acid sequence shown below, in which signal peptides are underlined and bolded: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 23) 
               
               
                 M   KFISTFLTFILAAVSVTA   NPQENFLKCFSEYIPNNPANPKFIYTQHDQ 
               
               
                   
               
               
                 LYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQI 
               
               
                   
               
               
                 RTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLG 
               
               
                   
               
               
                 EVYYWINEKNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNII 
               
               
                   
               
               
                 DAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSK 
               
               
                   
               
               
                 STIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHG 
               
               
                   
               
               
                 KNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTI 
               
               
                   
               
               
                 FYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKIL 
               
               
                   
               
               
                 EKLYEEDVGVGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWE 
               
               
                   
               
               
                 KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNN 
               
               
                   
               
               
                 YTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPPHHH   HDE     
               
               
                   
               
               
                     L   . 
               
            
           
         
       
     
     A non-limiting example of a nucleotide sequence encoding SEQ ID NO: 23, in which sequences encoding signal peptides are underlined and bolded, is shown below: 
     
       
         
           
               
               
            
               
                 (SEQ ID NO: 24) 
                   
               
               
                 atg   aagtttatcagtaccttcttgacctttatcttggccgctgtctccgtaaccgct   aacccgcaagaaaactttctaaaatgcttttct 
                   
               
               
                   
               
               
                 gaatacattcctaacaaccctgccaacccgaagtttatctacacacaacacgatcaattgtatatgagcgtgttgaatagtacaatacaga 
               
               
                   
               
               
                 acctgaggtttacatccgacacaacgccgaaaccgctagtgatcgtcacaccctccaacgtaagccacattcaggcaagcattttatgc 
               
               
                   
               
               
                 agcaagaaagtcggactgcagataaggacgaggtccggaggacacgacgccgaagggatgagctatatctcccaggtaccttttgt 
               
               
                   
               
               
                 ggtggtagacttgagaaatatgcactctatcaagatagacgttcactcccaaaccgcttgggttgaggcgggagccacccttggtgag 
               
               
                   
               
               
                 gtctactactggatcaacgaaaagaatgaaaattttagctttcctgggggatattgcccaactgtaggtgttggcggccacttctcaggag 
               
               
                   
               
               
                 gcggttatggggccttgatgcgtaactacggacttgcggccgacaacattatagacgcacatctagtgaatgtagacggcaaagtttta 
               
               
                   
               
               
                 gacaggaagagcatgggtgaggatcttttttgggcaattagaggcggagggggagaaaattttggaattatcgctgcttggaaaattaa 
               
               
                   
               
               
                 gctagttgcggtaccgagcaaaagcactatattctctgtaaaaaagaacatggagatacatggtttggtgaagctttttaataagtggcaa 
               
               
                   
               
               
                 aacatcgcgtacaagtacgacaaagatctggttctgatgacgcattttataacgaaaaatatcaccgacaaccacggaaaaaacaaaa 
               
               
                   
               
               
                 ccacagtacatggctacttctctagtatatttcatgggggagtcgattctctggttgatttaatgaacaaatcattcccagagttgggtata 
               
               
                   
               
               
                 aagaagacagactgtaaggagttctcttggattgacacaactatattctattcaggcgtagtcaactttaacacggcgaatttcaaaaaaga 
               
               
                   
               
               
                 gatccttctggacagatccgcaggtaagaaaactgcgttctctatcaaattggactatgtgaagaagcctattcccgaaaccgcgatggt 
               
               
                   
               
               
                 caagatacttgagaaattatacgaggaagatgtgggagttggaatgtacgtactttatccctatggtgggataatggaagaaatcagcga 
               
               
                   
               
               
                 gagcgccattccatttccccatcgtgccggcatcatgtacgagctgtggtatactgcgagttgggagaagcaagaagacaacgaaaa 
               
               
                   
               
               
                 gcacattaactgggtcagatcagtttacaatttcaccaccccatacgtgtcccagaatccgcgtctggcttacttgaactaccgtgatcttg 
               
               
                   
               
               
                 acctgggtaaaacgaacccggagtcacccaacaattacactcaagctagaatctggggagagaaatactttgggaagaacttcaaca 
               
               
                   
               
               
                 ggttagtaaaggttaaaaccaaggcagatccaaacaacttttttagaaatgaacaatccattcccccgctacccccgcaccatcac   cat     
               
               
                   
               
               
                     gatgaatta   . 
               
            
           
         
       
     
     In some embodiments, a  C. sativa  THCAS comprises the amino acid sequence set forth in UniProtKB—Q8GTB6 (SEQ ID NO: 14) in which the signal peptide is underlined and bolded: 
                    (SEQ ID NO: 14)       M   NCSAFSFWFVCKIIFFFLSFHIQISIA   NPRENFLKCFSKHIPNNVANPK               LVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCS               KKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVE               AGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLA               ADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVA               VPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITD               NHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDT               TIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKI               LEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWE               KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNY               TQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPPHHH.            
In some embodiments, a THCAS comprises the sequence shown below:
 
     
       
         
           
               
            
               
                 (SEQ ID NO: 214) 
               
               
                 NPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTT 
               
               
                   
               
               
                 PKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFV 
               
               
                   
               
               
                 VVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPT 
               
               
                   
               
               
                 VGVGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFW 
               
               
                   
               
               
                 AIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQN 
               
               
                   
               
               
                 IAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLM 
               
               
                   
               
               
                 NKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKK 
               
               
                   
               
               
                 TAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISE 
               
               
                   
               
               
                 SAIPFPHRAGIMYELWYTASWEKQEDNEKHINWVRSVYNFTTPYVSQNPR 
               
               
                   
               
               
                 LAYLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPN 
               
               
                   
               
               
                 NFFRNEQSIPPLPPHHH 
               
            
           
         
       
     
     Additional non-limiting examples of THCAS enzymes may also be found in U.S. Pat. No. 9,512,391 and US Publication No. 2018/0179564, which are incorporated by reference in this application in their entireties. 
     Cannabidiolic Acid Synthase (CBDAS) 
     A host cell described in this application may comprise a TS that is a cannabidiolic acid synthase (CBDAS). As used in this application, a “CBDAS” refers to an enzyme that is capable of catalyzing oxidative cyclization of a prenyl moiety (e.g., terpene) of a compound of Formula (8) to produce a compound of Formula 9. In some embodiments, a compound of Formula 9 is a compound of Formula (9a) (cannabidiolic acid (CBDA)), CBDVA, or CBDP. A CBDAS may use cannabigerolic acid (CBGA) or cannabinerolic acid as a substrate. In some embodiments, a cannabidiolic acid synthase is capable of oxidative cyclization of cannabigerolic acid (CBGA) to produce cannabidiolic acid (CBDA). In some embodiments, the CBDAS may catalyze the oxidative cyclization of other substrates, such as 3-geranyl-2,4-dihydro-6-alkylbenzoic acids like cannabigerovarinic acid (CBVGA). In some embodiments, the CBDAS exhibits specificity for CBGA substrates. 
     In some embodiments, a CBDAS is from  Cannabis . In  C. sativa , CBDAS is encoded by the CBDAS gene and is a flavoenzyme. A non-limiting example of an amino acid sequence comprising a CBDAS is provided by UniProtKB—A6P6V9 (SEQ ID NO: 13) from  C. sativa  in which the signal peptide is underlined and bolded: 
     
       
         
           
               
            
               
                 M   KCSTFSFWFVCKIIFFFFSFNIQTSIA   NPRENFLKCFSQYIPNNATNLK 
               
               
                   
               
               
                 LVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCS 
               
               
                   
               
               
                 KKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVE 
               
               
                   
               
               
                 AGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLA 
               
               
                   
               
               
                 ADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVA 
               
               
                   
               
               
                 VPKSTMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDN 
               
               
                   
               
               
                 QGKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTI 
               
               
                   
               
               
                 IFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQIL 
               
               
                   
               
               
                 EKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSWEK 
               
               
                   
               
               
                 QEDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYT 
               
               
                   
               
               
                 QARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPLPRHRH 
               
            
           
         
       
     
     In some embodiments, a CBDAS comprises the sequence shown below: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 215) 
               
               
                 NPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTT 
               
               
                   
               
               
                 PKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFV 
               
               
                   
               
               
                 IVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPT 
               
               
                   
               
               
                 VCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFW 
               
               
                   
               
               
                 ALRGGGAESFGIIVAWKIRLVAVPKSTMFSVKKIMEIHELVKLVNKWQNI 
               
               
                   
               
               
                 AYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMN 
               
               
                   
               
               
                 KSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNG 
               
               
                   
               
               
                 AFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISES 
               
               
                   
               
               
                 AIPFPHRAGILYELWYICSWEKQEDNEKHLNWIRNIYNFMTPYVSKNPRL 
               
               
                   
               
               
                 AYLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNN 
               
               
                   
               
               
                 FFRNEQSIPPLPRHRH 
               
            
           
         
       
     
     Additional non-limiting examples of CBDAS enzymes may also be found in U.S. Pat. No. 9,512,391 and US Publication No. 2018/0179564, which are incorporated by reference in this application in their entireties. 
     Cannabichromenic Acid Synthase (CBCAS) 
     A host cell described in this application may comprise a TS that is a cannabichromenic acid synthase (CBCAS). As used in this application, a “CBCAS” refers to an enzyme that is capable of catalyzing oxidative cyclization of a prenyl moiety (e.g., terpene) of a compound of Formula (8) to produce a compound of Formula (11). In some embodiments, a compound of Formula (11) is a compound of Formula (11a) (cannabichromenic acid (CBCA)), CBCVA, or a compound of Formula (8) with R as a C7 alkyl (heptyl) group. A CBCAS may use cannabigerolic acid (CBGA) as a substrate. In some embodiments, a CBCAS produces cannabichromenic acid (CBCA) from cannabigerolic acid (CBGA). In some embodiments, the CBCAS may catalyze the oxidative cyclization of other substrates, such as 3-geranyl-2,4-dihydro-6-alkylbenzoic acids like cannabigerovarinic acid (CBVGA), or a substrate of Formula (8) with R as a C7 alkyl (heptyl) group. In some embodiments, the CBCAS exhibits specificity for CBGA substrates. 
     In some embodiments, a CBCAS is from  Cannabis . A  C. sativa  CBCAS has the amino acid sequence as follows, in which the signal peptide is underlined and bolded: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 15) 
               
               
                 M   NCSTFSFWFVCKIIFFFLSFNIQISIA   NPQENFLKCFSEYIPNNPANPK 
               
               
                   
               
               
                 FIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCS 
               
               
                   
               
               
                 KKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVE 
               
               
                   
               
               
                 AGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLA 
               
               
                   
               
               
                 ADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAACKIKLVV 
               
               
                   
               
               
                 VPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITD 
               
               
                   
               
               
                 NHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDT 
               
               
                   
               
               
                 TIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKI 
               
               
                   
               
               
                 LEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWE 
               
               
                   
               
               
                 KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNY 
               
               
                   
               
               
                 TQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPPRHH. 
               
            
           
         
       
     
     In some embodiments, a CBCAS comprises the sequence shown below: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 33) 
               
               
                 NPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTT 
               
               
                   
               
               
                 PKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFA 
               
               
                   
               
               
                 IVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPT 
               
               
                   
               
               
                 VGVGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFW 
               
               
                   
               
               
                 AIRGGGGENFGIIAACKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQN 
               
               
                   
               
               
                 IAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLM 
               
               
                   
               
               
                 NKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKK 
               
               
                   
               
               
                 TAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISE 
               
               
                   
               
               
                 SAIPFPHRAGIMYELWYTATWEKQEDNEKHINWVRSVYNFTTPYVSQNPR 
               
               
                   
               
               
                 LAYLNYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPN 
               
               
                   
               
               
                 NFFRNEQSIPPLPPRHH. 
               
            
           
         
       
     
     In other embodiments, a CBCAS may be a CBCAS described in and incorporated by reference from U.S. Pat. No. 9,359,625. 
     In some embodiments, a CBCAS may be a  C. sativa  enzyme that also exhibits THCAS activity, such as a THCAS corresponding to Uniprot KB Accession No.: I1V0C5. In some embodiments, a CBCAS may be a  C. sativa  THCAS corresponding to any of SEQ ID NOs: 20-24. 
     As described in the Examples section, it was surprisingly discovered that multiple fungal enzymes, including enzymes of the  Aspergillus  family, such as an enzyme from  A. niger  (mold), are capable of catalyzing the conversion of a compound of Formula (8) to produce a compound of Formula (11), and, in some cases, also to produce a compound of Formula (10) and/or a compound of Formula (9). Whereas  Cannabis  plants have been under artificially high selection pressure to produce cannabinoids through human intervention for centuries, fungal species, such as the  A. niger  mold, have not been subjected to selection pressure for cannabinoid production. Therefore, without being bound by a particular theory, the fungal CBCASs, such as the  A. niger  CBCAS, disclosed in this application may be useful for engineering to alter the activity and or abundance of the TS (e.g., change the product profile, substrate profile, and/or kinetics (e.g., Kcat/Vmax and/or Kd) of the TS). It was also surprisingly found, as shown in the Examples section, that many of the fungal enzymes, including enzymes of the  Aspergillus  family, such as the  A. niger  enzyme, identified in this disclosure exhibit CBCAS activity, CBCVAS activity, or even both. Some of these enzymes additionally exhibited THCAS activity, THCVAS activity, CBDAS activity, or a combination thereof. 
     In some embodiments, a CBCAS from  A. niger  comprises the amino acid sequence shown below: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 25) 
               
               
                 GNTTSIAGRDCLISALGGNSALAVFPNELLWTADVHEYNLNLPVTPAAIT 
               
               
                   
               
               
                 YPETAAQIAGVVKCASDYDYKVQARSGGHSFGNYGLGGADGAVVVDMKHF 
               
               
                   
               
               
                 TQFSMDDETYEAVIGPGTTLNDVDIELYNNGKRAMAHGVCPTIKTGGHFT 
               
               
                   
               
               
                 IGGLGPTARQWGLALDHVEEVEVVLANSSIVRASNTQNQDVFFAVKGAAA 
               
               
                   
               
               
                 NFGIVTEFKVRTEPAPGLAVQYSYTFNLGSTAEKAQFVKDWQSFISAKNL 
               
               
                   
               
               
                 TRQFYNNMVIFDGDIILEGLFFGSKEQYDALGLEDHFAPKNPGNILVLTD 
               
               
                   
               
               
                 WLGMVGHALEDTILKLVGNTPTWFYAKSLGFRQDTLIPSAGIDEFFEYIA 
               
               
                   
               
               
                 NHTAGTPAWFVTLSLEGGAINDVAEDATAYAHRDVLFWVQLFMVNPVGPI 
               
               
                   
               
               
                 SDTTYEFTDGLYDVLARAVPESVGHAYLGCPDPRMEDAQQKYWRTNLPRL 
               
               
                   
               
               
                 QELKEELDPKNTFHHPQGVMPA. 
               
            
           
         
       
     
     A non-limiting example of a nucleic acid sequence encoding SEQ ID NO: 25 for expression in  S. cerevisiae  is: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 26) 
               
               
                 ggtaatacgacctctattgccggcagagattgtttgatctcagctttagg 
               
               
                   
               
               
                 tggtaactccgctcttgcagtttttccaaacgagttgctatggacagctg 
               
               
                   
               
               
                 acgtacacgaatataatctgaacttgcctgtcactcccgctgctataacc 
               
               
                   
               
               
                 tacccagaaaccgccgctcagattgccggtgtggttaagtgcgcttctga 
               
               
                   
               
               
                 ttacgactataaagtccaagcaaggtccggaggtcatagtttcggtaatt 
               
               
                   
               
               
                 acggcttgggtggagctgacggtgcagttgtcgttgatatgaagcacttc 
               
               
                   
               
               
                 actcaattttcgatggacgatgaaacttacgaagctgttatcggtccagg 
               
               
                   
               
               
                 tacaactttaaacgatgtcgacatcgaattgtacaacaacggtaaaagag 
               
               
                   
               
               
                 ccatggctcatggtgtatgtccaaccattaagactggtggtcacttcacc 
               
               
                   
               
               
                 atcggtggtctaggacctacggctcgtcaatggggtctggctttggacca 
               
               
                   
               
               
                 tgtcgaggaagttgaagttgtgttagctaactctagcattgttagagcct 
               
               
                   
               
               
                 ctaatacacaaaatcaagatgttttctttgcagtcaagggtgctgctgct 
               
               
                   
               
               
                 aacttcggaatcgtcactgaatttaaagttagaactgaaccagccccagg 
               
               
                   
               
               
                 tttggctgtacagtactcctataccttcaacttgggttcaactgccgaga 
               
               
                   
               
               
                 aggctcaattcgttaaggattggcaatctttcatttcggctaagaaccta 
               
               
                   
               
               
                 accagacaattttataataacatggtcatttttgatggtgacataatctt 
               
               
                   
               
               
                 ggaaggtttattcttcggtagcaaggaacaatacgacgccttgggccttg 
               
               
                   
               
               
                 aagatcacttcgcaccaaagaatccaggtaacatattggttttaacagat 
               
               
                   
               
               
                 tggctaggcatggtgggtcacgcattggaagacactattttaaaattggt 
               
               
                   
               
               
                 cggtaataccccaacatggttctatgctaagtccttgggttttagacaag 
               
               
                   
               
               
                 acactctgatcccttctgccggtattgacgaatttttcgaatacattgct 
               
               
                   
               
               
                 aaccataccgccggcactcctgcttggtttgttactttgtccttagaggg 
               
               
                   
               
               
                 tggtgctatcaacgatgtcgcagaagatgctacggcctatgctcacagag 
               
               
                   
               
               
                 atgttttgttctgggtccaactattcatggttaatccagtcggtcctatc 
               
               
                   
               
               
                 tctgacactacctacgagtttacagacggcttgtacgatgtgttggcccg 
               
               
                   
               
               
                 tgctgttccagaaagcgtgggacatgcttaccttggttgtccagatccaa 
               
               
                   
               
               
                 gaatggaagacgctcaacagaagtattggcgtaccaatttgccccgtctg 
               
               
                   
               
               
                 caagaactaaaggaagagttggatccaaaaaacaccttccatcacccaca 
               
               
                   
               
               
                 gggtgttatgccagcttaa. 
               
            
           
         
       
     
     In some embodiments, a CBCAS from  A. niger  comprises the amino acid sequence shown below (corresponding to UniProt accession no. A0A254UC34): 
     
       
         
           
               
            
               
                 (SEQ ID NO: 27) 
               
               
                 MGNTTSIAGRDCLISALGGNSALAVFPNELLWTADVHEYNLNLPVTPAAI 
               
               
                   
               
               
                 TYPETAAQIAGVVKCASDYDYKVQARSGGHSFGNYGLGGADGAVVVDMKH 
               
               
                   
               
               
                 FTQFSMDDETYEAVIGPGTTLNDVDIELYNNGKRAMAHGVCPTIKTGGHF 
               
               
                   
               
               
                 TIGGLGPTARQWGLALDHVEEVEVVLANSSIVRASNTQNQDVFFAVKGAA 
               
               
                   
               
               
                 ANFGIVTEFKVRTEPAPGLAVQYSYTFNLGSTAEKAQFVKDWQSFISAKN 
               
               
                   
               
               
                 LTRQFYNNMVIFDGDIILEGLFFGSKEQYDALGLEDHFAPKNPGNILVLT 
               
               
                   
               
               
                 DWLGMVGHALEDTILKLVGNTPTWFYAKSLGFRQDTLIPSAGIDEFFEYI 
               
               
                   
               
               
                 ANHTAGTPAWFVTLSLEGGAINDVAEDATAYAHRDVLFWVQLFMVNPVGP 
               
               
                   
               
               
                 ISDTTYEFTDGLYDVLARAVPESVGHAYLGCPDPRMEDAQQKYWRTNLPR 
               
               
                   
               
               
                 LQELKEELDPKNTFHHPQGVMPA. 
               
            
           
         
       
     
     A non-limiting example of a nucleic acid sequence encoding SEQ ID NO: 27 for expression in  S. cerevisiae  is: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 28) 
               
               
                 atgggtaatacgacctctattgccggcagagattgtttgatctcagcttt 
               
               
                   
               
               
                 aggtggtaactccgctcttgcagtttttccaaacgagttgctatggacag 
               
               
                   
               
               
                 ctgacgtacacgaatataatctgaacttgcctgtcactcccgctgctata 
               
               
                   
               
               
                 acctacccagaaaccgccgctcagattgccggtgtggttaagtgcgcttc 
               
               
                   
               
               
                 tgattacgactataaagtccaagcaaggtccggaggtcatagtttcggta 
               
               
                   
               
               
                 attacggcttgggtggagctgacggtgcagttgtcgttgatatgaagcac 
               
               
                   
               
               
                 ttcactcaattttcgatggacgatgaaacttacgaagctgttatcggtcc 
               
               
                   
               
               
                 aggtacaactttaaacgatgtcgacatcgaattgtacaacaacggtaaaa 
               
               
                   
               
               
                 gagccatggctcatggtgtatgtccaaccattaagactggtggtcacttc 
               
               
                   
               
               
                 accatcggtggtctaggacctacggctcgtcaatggggtctggctttgga 
               
               
                   
               
               
                 ccatgtcgaggaagttgaagttgtgttagctaactctagcattgttagag 
               
               
                   
               
               
                 cctctaatacacaaaatcaagatgttttctttgcagtcaagggtgctgct 
               
               
                   
               
               
                 gctaacttcggaatcgtcactgaatttaaagttagaactgaaccagcccc 
               
               
                   
               
               
                 aggtttggctgtacagtactcctataccttcaacttgggttcaactgccg 
               
               
                   
               
               
                 agaaggctcaattcgttaaggattggcaatctttcatttcggctaagaac 
               
               
                   
               
               
                 ctaaccagacaattttataataacatggtcatttttgatggtgacataat 
               
               
                   
               
               
                 cttggaaggtttattcttcggtagcaaggaacaatacgacgccttgggcc 
               
               
                   
               
               
                 ttgaagatcacttcgcaccaaagaatccaggtaacatattggttttaaca 
               
               
                   
               
               
                 gattggctaggcatggtgggtcacgcattggaagacactattttaaaatt 
               
               
                   
               
               
                 ggtcggtaataccccaacatggttctatgctaagtccttgggttttagac 
               
               
                   
               
               
                 aagacactctgatcccttctgccggtattgacgaatttttcgaatacatt 
               
               
                   
               
               
                 gctaaccataccgccggcactcctgcttggtttgttactttgtccttaga 
               
               
                   
               
               
                 gggtggtgctatcaacgatgtcgcagaagatgctacggcctatgctcaca 
               
               
                   
               
               
                 gagatgttttgttctgggtccaactattcatggttaatccagtcggtcct 
               
               
                   
               
               
                 atctctgacactacctacgagtttacagacggcttgtacgatgtgttggc 
               
               
                   
               
               
                 ccgtgctgttccagaaagcgtgggacatgcttaccttggttgtccagatc 
               
               
                   
               
               
                 caagaatggaagacgctcaacagaagtattggcgtaccaatttgccccgt 
               
               
                   
               
               
                 ctgcaagaactaaaggaagagttggatccaaaaaacaccttccatcaccc 
               
               
                   
               
               
                 acagggtgttatgccagcttaa. 
               
            
           
         
       
     
     In some embodiments, a CBCAS comprises each of: SEQ ID NO: 25; the MFalpha2 signal peptide; and the HDEL signal peptide. In some embodiments, such a CBCAS comprises the amino acid sequence shown below, in which signal peptides are underlined and bolded: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 29) 
               
               
                 M   KFISTFLTFILAAVSVTA   GNTTSIAGRDCLISALGGNSALAVFPNELLW 
               
               
                   
               
               
                 TADVHEYNLNLPVTPAAITYPETAAQIAGVVKCASDYDYKVQARSGGHSF 
               
               
                   
               
               
                 GNYGLGGADGAVVVDMKHFTQFSMDDETYEAVIGPGTTLNDVDIELYNNG 
               
               
                   
               
               
                 KRAMAHGVCPTIKTGGHFTIGGLGPTARQWGLALDHVEEVEVVLANSSIV 
               
               
                   
               
               
                 RASNTQNQDVFFAVKGAAANFGIVTEFKVRTEPAPGLAVQYSYTFNLGST 
               
               
                   
               
               
                 AEKAQFVKDWQSFISAKNLTRQFYNNMVIFDGDIILEGLFFGSKEQYDAL 
               
               
                   
               
               
                 GLEDHFAPKNPGNILVLTDWLGMVGHALEDTILKLVGNTPTWFYAKSLGF 
               
               
                   
               
               
                 RQDTLIPSAGIDEFFEYIANHTAGTPAWFVTLSLEGGAINDVAEDATAYA 
               
               
                   
               
               
                 HRDVLFWVQLFMVNPVGPISDTTYEFTDGLYDVLARAVPESVGHAYLGCP 
               
               
                   
               
               
                 DPRMEDAQQKYWRTNLPRLQELKEELDPKNTFHHPQGVMPA   HDEL   . 
               
            
           
         
       
     
     A non-limiting example of a nucleic acid sequence encoding SEQ ID NO: 29 is shown below, in which sequences encoding signal peptides are underlined and bolded: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 30) 
               
               
                 atg   aagtttatcagtaccttcttgacctttatcttggccgctgtctccgt     
               
               
                   
               
               
                     aaccgct   ggtaatacgacctctattgccggcagagattgtttgatctcag 
               
               
                   
               
               
                 ctttaggtggtaactccgctcttgcagtttttccaaacgagttgctatgg 
               
               
                   
               
               
                 acagctgacgtacacgaatataatctgaacttgcctgtcactcccgctgc 
               
               
                   
               
               
                 tataacctacccagaaaccgccgctcagattgccggtgtggttaagtgcg 
               
               
                   
               
               
                 cttctgattacgactataaagtccaagcaaggtccggaggtcatagtttc 
               
               
                   
               
               
                 ggtaattacggcttgggtggagctgacggtgcagttgtcgttgatatgaa 
               
               
                   
               
               
                 gcacttcactcaattttcgatggacgatgaaacttacgaagctgttatcg 
               
               
                   
               
               
                 gtccaggtacaactttaaacgatgtcgacatcgaattgtacaacaacggt 
               
               
                   
               
               
                 aaaagagccatggctcatggtgtatgtccaaccattaagactggtggtca 
               
               
                   
               
               
                 cttcaccatcggtggtctaggacctacggctcgtcaatggggtctggctt 
               
               
                   
               
               
                 tggaccatgtcgaggaagttgaagttgtgttagctaactctagcattgtt 
               
               
                   
               
               
                 agagcctctaatacacaaaatcaagatgttttctttgcagtcaagggtgc 
               
               
                   
               
               
                 tgctgctaacttcggaatcgtcactgaatttaaagttagaactgaaccag 
               
               
                   
               
               
                 ccccaggtttggctgtacagtactcctataccttcaacttgggttcaact 
               
               
                   
               
               
                 gccgagaaggctcaattcgttaaggattggcaatctttcatttcggctaa 
               
               
                   
               
               
                 gaacctaaccagacaattttataataacatggtcatttttgatggtgaca 
               
               
                   
               
               
                 taatcttggaaggtttattcttcggtagcaaggaacaatacgacgccttg 
               
               
                   
               
               
                 ggccttgaagatcacttcgcaccaaagaatccaggtaacatattggtttt 
               
               
                   
               
               
                 aacagattggctaggcatggtgggtcacgcattggaagacactattttaa 
               
               
                   
               
               
                 aattggtcggtaataccccaacatggttctatgctaagtccttgggtttt 
               
               
                   
               
               
                 agacaagacactctgatcccttctgccggtattgacgaatttttcgaata 
               
               
                   
               
               
                 cattgctaaccataccgccggcactcctgcttggtttgttactttgtcct 
               
               
                   
               
               
                 tagagggtggtgctatcaacgatgtcgcagaagatgctacggcctatgct 
               
               
                   
               
               
                 cacagagatgttttgttctgggtccaactattcatggttaatccagtcgg 
               
               
                   
               
               
                 tcctatctctgacactacctacgagtttacagacggcttgtacgatgtgt 
               
               
                   
               
               
                 tggcccgtgctgttccagaaagcgtgggacatgcttaccttggttgtcca 
               
               
                   
               
               
                 gatccaagaatggaagacgctcaacagaagtattggcgtaccaatttgcc 
               
               
                   
               
               
                 ccgtctgcaagaactaaaggaagagttggatccaaaaaacaccttccatc 
               
               
                   
               
               
                 acccacagggtgttatgccagcttaa   catgatgaatta   . 
               
               
                   
               
            
           
         
       
     
     In some embodiments, a TS comprises a nucleic acid or protein sequence that is at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or is 100% identical, including all values in between, to one or more of SEQ ID NOs: 20-30 or 34-173, to any one of the sequences in Table 15, or to any TS disclosed in this application. In some embodiments, a TS comprises a nucleic acid or protein sequence that is at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or is 100% identical, including all values in between, to one or more of SEQ ID NOs: 25, 26, 27, 28, 35, 56, 64, 85, 92, 94, 95, 105, 126, 134, 155, 162, 164, and 165. In some embodiments, a TS comprises a nucleic acid or protein sequence that is at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or is 100% identical, including all values in between, to one or more of SEQ ID NOs: 25, 26, 27, 28, 35, 42, 56, 60, 64, 105, 85, 92, 94, 95, 112, 126, 130, 134, 155, 162, 164, 165. In some embodiments, a TS comprises a nucleic acid or protein sequence that is at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or is 100% identical, including all values in between, to one or more of SEQ ID NOs: 25, 26, 27, 28, 35, 42, 56, 60, 64, 105, 85, 89, 92, 93, 94, 95, 96, 97, 102, 112, 126, 130, 134, 155, 159, 162, 163, 164, 165, 166, 167, and 172. 
     In some embodiments, a TS comprises a sequence that is at most 5%, at most 10%, at most 15%, at most 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%, at most 60%, at most 65%, at most 70%, at most 71%, at most 72%, at most 73%, at most 74%, at most 75%, at most 76%, at most 77%, at most 78%, at most 79%, at most 80%, at most 81%, at most 82%, at most 83%, at most 84%, at most 85%, at most 86%, at most 87%, at most 88%, at most 89%, at most 90%, at most 91%, at most 92%, at most 93%, at most 94%, at most 95%, at most 96%, at most 97%, at most 98%, at most 99%, or is 100% identical, including all values in between, to one or more of SEQ ID NOs: 20-30 or 34-173, to any one of the sequences in Table 15, or to any TS disclosed in this application. In some embodiments, a TS comprises a sequence that is 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical, including all values in between, to one or more of SEQ ID NOs: 20-30 or 34-173, to any one of the sequences in Table 15, or to any TS disclosed in this application. 
     In some embodiments, a TS sequence that is at least 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to SEQ ID NO: 29 includes a signal peptide that comprises SEQ ID NO: 16 or a sequence that has no more than two amino acid substitutions, insertions, additions, or deletions relative to the sequence of SEQ ID NO: 16. In some embodiments, the signal peptide that comprises SEQ ID NO: 16 or a sequence that has no more than two amino acid substitutions, insertions, additions, or deletions relative to the sequence of SEQ ID NO: 16 is located at the N-terminus of the TS sequence. For example, the signal peptide that comprises SEQ ID NO: 16 or a sequence that has no more than two amino acid substitutions, insertions, additions, or deletions relative to the sequence of SEQ ID NO: 16 may start at position 2 of the TS sequence following a methionine residue. 
     In some embodiments, a TS sequence that is at least 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to SEQ ID NO: 29 includes a signal peptide that comprises SEQ ID NO: 17 or a sequence that has no more than one amino acid substitution, insertion, addition, or deletion relative to the sequence of SEQ ID NO: 17. In some embodiments, the signal peptide that comprises SEQ ID NO: 17 or a sequence that has no more than one amino acid substitution, insertion, addition, or deletion relative to the sequence of SEQ ID NO: 17 is located at the C-terminus of the sequence that is at least 90% identical to SEQ ID NO: 29. 
     In some embodiments, a TS comprises a sequence that is at least 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to any one of SEQ ID NO: 25, 27 or 104-173 wherein the sequence is linked to one or more signal peptides. In some embodiments, a signal peptide that comprises SEQ ID NO: 16 or a sequence that has no more than two amino acid substitutions, insertions, additions, or deletions relative to the sequence of SEQ ID NO: 16 is linked to the N-terminus of the sequence that is at least 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to any one of SEQ ID NO: 25, 27 or 104-173. In some embodiments, the N-terminal methionine residue of any one of SEQ ID NOs: 27 or 104-173 is not included when the sequence is linked to an N-terminal signal peptide. In some embodiments, a methionine residue is added to the N-terminus of the N-terminal signal peptide (e.g., SEQ ID NO: 16). In some embodiments, a signal peptide that comprises SEQ ID NO: 17 or a sequence that has no more than one amino acid substitution, insertion, addition, or deletion relative to the sequence of SEQ ID NO: 17 is linked to the carboxyl terminus of the sequence that is at least 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to SEQ ID NO: 25, 27 or 104-173. 
     In some embodiments, a TS comprises a sequence that is at least 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 155, 159, 162, 163, 164, 165, 166, 167, and 172, wherein the sequence is linked to one or more signal peptides. In some embodiments, a signal peptide that comprises SEQ ID NO: 16 or a sequence that has no more than two amino acid substitutions, insertions, additions, or deletions relative to the sequence of SEQ ID NO: 16 is linked to the N-terminus of the sequence that is at least 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 155, 159, 162, 163, 164, 165, 166, 167, and 172. In some embodiments, the N-terminal methionine residue of any one of SEQ ID NOs: 27, 105, 112, 126, 130, 134, 155, 159, 162, 163, 164, 165, 166, 167, and 172 is not included when the sequence is linked to an N-terminal signal peptide. In some embodiments, a methionine residue is added to the N-terminus of the N-terminal signal peptide (e.g., SEQ ID NO: 16). In some embodiments, a signal peptide that comprises SEQ ID NO: 17 or a sequence that has no more than one amino acid substitution, insertion, addition, or deletion relative to the sequence of SEQ ID NO: 17 is linked to the carboxyl terminus of the sequence that is at least 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to any one of SEQ ID NOs: 25, 27, 105, 112, 126, 130, 134, 155, 159, 162, 163, 164, 165, 166, 167, and 172. 
     In some embodiments, relative to SEQ ID NO: 21, a TS comprises an amino acid substitution, deletion, or insertion at a residue corresponding to position 1, 2, 3, 4, 5, 6, 8, 10, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, 29, 30, 31, 33, 34, 35, 37, 39, 41, 48, 49, 51, 55, 58, 60, 61, 62, 70, 72, 74, 75, 76, 81, 88, 89, 91, 94, 97, 100, 101, 102, 104, 105, 106, 108, 110, 111, 112, 113, 114, 115, 116, 117, 119, 122, 123, 125, 127, 130, 132, 133, 135, 137, 138, 139, 140, 141, 142, 145, 147, 149, 150, 164, 165, 168, 169, 172, 173, 175, 176, 177, 180, 181, 183, 184, 185, 187, 193, 201, 208, 209, 212, 214, 215, 217, 222, 225, 226, 227, 229, 231, 233, 235, 236, 238, 239, 241, 242, 243, 244, 245, 246, 247, 250, 251, 253, 254, 255, 256, 257, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 277, 278, 279, 281, 282, 283, 284, 286, 287, 288, 290, 292, 293, 294, 295, 297, 298, 299, 301, 302, 309, 310, 311, 312, 315, 317, 322, 323, 324, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 344, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 357, 361, 362, 365, 366, 368, 369, 370, 371, 372, 373, 374, 376, 377, 379, 380, 381, 382, 383, 384, 385, 386, 387, 389, 394, 396, 401, 402, 411, 412, 414, 415, 416, 418, 419, 420, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 439, 440, 441, 447, 448, 451, 452, 459, 461, 463, 464, 465, 467, 468, 469, 470, 471, 473, 474, 477, 484, 485, 488, 492, 496, 497, 500, 505, 511, 513, 514, 515, 516, and/or 517 in SEQ ID NO: 21. In some embodiments, a TS comprises the amino acid residue that is present in SEQ ID NO: 25 at a position corresponding to position 1, 2, 3, 4, 5, 6, 8, 10, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, 29, 30, 31, 33, 34, 35, 37, 39, 41, 48, 49, 51, 55, 58, 60, 61, 62, 70, 72, 74, 75, 76, 81, 88, 89, 91, 94, 97, 100, 101, 102, 104, 105, 106, 108, 110, 111, 112, 113, 114, 115, 116, 117, 119, 122, 123, 125, 127, 130, 132, 133, 135, 137, 138, 139, 140, 141, 142, 145, 147, 149, 150, 164, 165, 168, 169, 172, 173, 175, 176, 177, 180, 181, 183, 184, 185, 187, 193, 201, 208, 209, 212, 214, 215, 217, 222, 225, 226, 227, 229, 231, 233, 235, 236, 238, 239, 241, 242, 243, 244, 245, 246, 247, 250, 251, 253, 254, 255, 256, 257, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 277, 278, 279, 281, 282, 283, 284, 286, 287, 288, 290, 292, 293, 294, 295, 297, 298, 299, 301, 302, 309, 310, 311, 312, 315, 317, 322, 323, 324, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 344, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 357, 361, 362, 365, 366, 368, 369, 370, 371, 372, 373, 374, 376, 377, 379, 380, 381, 382, 383, 384, 385, 386, 387, 389, 394, 396, 401, 402, 411, 412, 414, 415, 416, 418, 419, 420, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 439, 440, 441, 447, 448, 451, 452, 459, 461, 463, 464, 465, 467, 468, 469, 470, 471, 473, 474, 477, 484, 485, 488, 492, 496, 497, 500, 505, 511, 513, 514, 515, 516, and/or 517 in SEQ ID NO: 21. 
     Examples 1 and 3 describe the identification of fungal candidate TSs that were surprisingly effective in producing CBCA. Table 14 provides non-limiting examples of sequence motifs that were identified as being enriched in the sequences of candidate TSs that were effective in producing CBCA. In some embodiments, a TS includes one or more of the following motifs, provided in Table 14: KVQARSGGH (SEQ ID NO: 174), RASNTQNQD[VI][FL]FA[VI]K (SEQ ID NO: 176), CPTI[KR]TGGH (SEQ ID NO: 181), WFVTLSLEGGAINDV[AP]EDATAY[AG]H (SEQ ID NO: 184), P[IV]S[DQE]TTY[EDG]F[TA]DGLYDVLA[RQK]AVPES[VA]GHAYLGCPDP[RK]M (SEQ ID NO: 186), MKHF[TNS]QFSM (SEQ ID NO: 189), P[EQ][TS]A[EAD][QE]IA[GA][VI]VKC (SEQ ID NO: 193), RDCL[IV]SA[LV]GGN[SA]A[LH][AV][AV]F[PQ][ND][QE]LL[WY] (SEQ ID NO: 200), RT[EQ][PQ]APGLAVQYSY (SEQ ID NO: 207), and/or WQ[SA]FI[SA][AQ][KE]NLT[RW][QK]FY[NST]NM (SEQ ID NO: 211). In some embodiments, a TS includes the motif KVQARSGGH (SEQ ID NO: 174) at residues corresponding to residues 72-80 in SEQ ID NO: 27. 
     In some embodiments, a TS includes the motif RASNTQNQD[VI][FL]FA[VI]K (SEQ ID NO: 176) at residues corresponding to residues 183-197 in SEQ ID NO: 27. In some embodiments, the motif 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 176) 
               
               
                   
                 RASNTQNQD[VI][FL]FA[VI]K is 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 177) 
               
               
                   
                 RASNTQNQDVFFAVK, 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 178) 
               
               
                   
                 RASNTQNQDILFAVK, 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 179), 
               
               
                   
                 RASNTQNQDILFAIK 
               
               
                   
                 or 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 180) 
               
               
                   
                 RASNTQNQDVLFAVK. 
               
            
           
         
       
     
     In some embodiments, a TS includes the motif CPTI[KR]TGGH (SEQ ID NO: 181) at residues corresponding to residues 141-149 in SEQ ID NO: 27. In some embodiments, the motif CPTI[KR]TGGH (SEQ ID NO: 181) is CPTIKTGGH (SEQ ID NO: 182) or CPTIRTGGH (SEQ ID NO: 183). 
     In some embodiments, a TS includes the motif WFVTLSLEGGAINDV[AP]EDATAY[AG]H (SEQ ID NO: 184) at residues corresponding to residues 360-383 in SEQ ID NO: 27. In some embodiments, the motif 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 184) 
               
               
                   
                 WFVTLSLEGGAINDV[AP]EDATAY[AG]H is 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 185) 
               
               
                   
                 WFVTLSLEGGAINDVAEDATAYAH. 
               
            
           
         
       
     
     In some embodiments, a TS includes the motif P[IV]S[DQE]TTY[EDG]F[TA]DGLYDVLA[RQK]AVPES[VA]GHAYLGCPDP[RK]M (SEQ ID NO: 186) at residues corresponding to residues 400-436 in SEQ ID NO: 27. In some embodiments, the motif 
     
       
         
           
               
            
               
                 (SEQ ID NO: 186) 
               
               
                 P[IV]S[DQE]TTY[EDG]F[TA]DGLYDVLA[RQK]AVPES[VA]GHAY 
               
               
                   
               
               
                 LGCPDP[RK]M is 
               
               
                   
               
               
                 (SEQ ID NO: 187) 
               
               
                 PISDTTYEFTDGLYDVLARAVPESVGHAYLGCPDPRM 
               
               
                 or 
               
               
                   
               
               
                 (SEQ ID NO: 188) 
               
               
                 PISETTYEFTDGLYDVLARAVPESVGHAYLGCPDPRM. 
               
            
           
         
       
     
     In some embodiments, a TS includes the motif MKHF[TNS]QFSM (SEQ ID NO: 189) at residues corresponding to residues 98-106 in SEQ ID NO: 27. In some embodiments, the motif MKHF[TNS]QFSM (SEQ ID NO: 189) is MKHFTQFSM (SEQ ID NO: 190), MKHFSQFSM (SEQ ID NO: 191), or MKHFNQFSM (SEQ ID NO: 192). 
     In some embodiments, a TS includes the motif P[EQ][TS]A[EAD][QE]IA[GA][VI]VKC (SEQ ID NO: 193) at residues corresponding to residues 53-65 in SEQ ID NO: 27. In some embodiments, the motif 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 193) 
               
               
                   
                 P[EQ][TS]A[EAD][QE]IA[GA][VI]VKC is 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 194), 
               
               
                   
                 PETAEQIAGIVKC, 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 195) 
               
               
                   
                 PQSADEIAAVVKC, 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 196) 
               
               
                   
                 PETAAQIAGVVKC, 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 197) 
               
               
                   
                 PQSAEEIAAVVKC, 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 198) 
               
               
                   
                 PETAEQIAGVVKC, 
               
               
                   
                 or 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 199) 
               
               
                   
                 PETAEQIAAVVKC. 
               
            
           
         
       
     
     In some embodiments, a TS includes the motif 
                    (SEQ ID NO: 200)       RDCL[IV]SA[LV]GGN[SA]A[LH][AV][AV]F[PQ][ND][QE]LL               [WY]            
at residues corresponding to residues 10-32 in SEQ ID NO: 27. In some embodiments, the motif RDCL[IV]SA[LV]GGN[SA]A[LH][AV][AV]F[PQ][ND][QE]LL[WY] (SEQ ID NO: 200) is RDCLISAVGGNAAHVAFQDQLLY (SEQ ID NO: 201), RDCLISALGGNSALAVFPNELLW (SEQ ID NO: 202), RDCLISALGGNSALAAFPNELLW (SEQ ID NO: 203), RDCLISALGGNSALAVFPNQLLW (SEQ ID NO: 204), RDCLISALGGNSALAAFPNQLLW (SEQ ID NO: 205), or RDCLVSALGGNSALAAFPNQLLW (SEQ ID NO: 206).
 
     In some embodiments, a TS includes the motif RT[EQ][PQ]APGLAVQYSY (SEQ ID NO: 207) at residues corresponding to residues 212-225 in SEQ ID NO: 27. In some embodiments, the motif RT[EQ][PQ]APGLAVQYSY (SEQ ID NO: 207) is RTEPAPGLAVQYSY (SEQ ID NO: 208), RTEQAPGLAVQYSY (SEQ ID NO: 209), or RTQPAPGLAVQYSY (SEQ ID NO: 210). 
     In some embodiments, a TS includes the motif WQ[SA]FI[SA][AQ][KE]NLT[RW][QK]FY[NST]NM (SEQ ID NO: 211) at residues corresponding to residues 242-259 in SEQ ID NO: 27. In some embodiments, the motif 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 211) 
               
               
                   
                 WQ[SA]FI[SA][AQ][KE]NLT[RW][QK]FY[NST]NM is 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 212) 
               
               
                   
                 WQSFISAKNLTRQFYNNM 
               
               
                   
                 or 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 213) 
               
               
                   
                 WQSFISAKNLTRQFYTNM. 
               
            
           
         
       
     
     In some embodiments, one or more of the motifs described above may contact the cofactor (FAD) binding site of the TS. For example, KVQARSGGH (SEQ ID NO: 174), CPTI[KR]TGGH (SEQ ID NO: 181), and P[IV]S[DQE]TTY[EDG]F[TA]DGLYDVLA[RQK]AVPES[VA]GHAYLGCPDP[RK]M (SEQ ID NO: 186), indicated by arrows in  FIG.  15   , are predicted to contact the cofactor binding site and may therefore influence cofactor binding. Without wishing to be bound by any theory, these motifs may be involved in modulating the redox potential of the cofactor and may be important for enzyme activity by regulating, for example, enzyme turnover. 
     In some embodiments, one or more of the motifs described above may line the cavity of the active site of the TS. For example, WQ[SA]FI[SA][AQ][KE]NLT[RW][QK]FY[NST]NM (SEQ ID NO: 211), indicated by an arrow in  FIG.  16   , is predicted to line the cavity of the active site. In some embodiments, motifs RT[EQ][PQ]APGLAVQYSY (SEQ ID NO: 207) and WFVTLSLEGGAINDV[AP]EDATAY[AG]H (SEQ ID NO: 184) may also line the cavity of the active site and be near the substrate binding pocket. Without wishing to be bound by any theory, these motifs may influence substrate or product specificity. 
     In some embodiments, a TS associated with this disclosure comprises one or more amino acid substitutions, deletions, additions, or insertions relative to the sequence of any of the TSs provided in this disclosure. In some embodiments, relative to the sequence of SEQ ID NO: 27, the TS comprises an amino acid substitution at a residue corresponding to position 25, 33, 35, 39, 43, 55, 57, 61, 62, 63, 71, 102, 112, 114, 122, 126, 129, 131, 161, 180, 183, 202, 256, 257, 260, 262, 280, 287, 295, 341, 353, 386, 392, 394, 398, 410, 423, 426, 446, 450, 456, 458, 466, 469, and/or 472 in SEQ ID NO: 27. In some embodiments, relative to the sequence of SEQ ID NO: 27, the TS comprises an amino acid substitution at a residue corresponding to position 33, 39, 55, 57, 61, 62, 63, 71, 112, 122, 126, 129, 131 180, 183, 202, 256, 257, 260, 287, 295, 341, 386, 392, 394, 398, 410, 423, 426, 450, and/or 472. 
     In some embodiments, the TS comprises: the amino acid A at a residue corresponding to position 25 in SEQ ID NO: 27; the amino acid D at a residue corresponding to position 33 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 35 in SEQ ID NO: 27; the amino acid F at a residue corresponding to position 39 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 43 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 55 in SEQ ID NO: 27; the amino acid Q at a residue corresponding to position 57 in SEQ ID NO: 27; the amino acid E at a residue corresponding to position 57 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 61 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 62 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 63 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 71 in SEQ ID NO: 27; the amino acid N at a residue corresponding to position 102 in SEQ ID NO: 27; the amino acid Q at a residue corresponding to position 102 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 102 in SEQ ID NO: 27; the amino acid V at a residue corresponding to position 112 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 112 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 114 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 122 in SEQ ID NO: 27; the amino acid G at a residue corresponding to position 122 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 122 in SEQ ID NO: 27; the amino acid E at a residue corresponding to position 122 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 126 in SEQ ID NO: 27; the amino acid R at a residue corresponding to position 126 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 126 in SEQ ID NO: 27; the amino acid K at a residue corresponding to position 126 in SEQ ID NO: 27; the amino acid D at a residue corresponding to position 126 in SEQ ID NO: 27; the amino acid W at a residue corresponding to position 129 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 131 in SEQ ID NO: 27; the amino acid K at a residue corresponding to position 161 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 180 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 183 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 202 in SEQ ID NO: 27; the amino acid G at a residue corresponding to position 202 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 202 in SEQ ID NO: 27; the amino acid F at a residue corresponding to position 256 in SEQ ID NO: 27; the amino acid M at a residue corresponding to position 256 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 257 in SEQ ID NO: 27; the amino acid M at a residue corresponding to position 260 in SEQ ID NO: 27; the amino acid F at a residue corresponding to position 260 in SEQ ID NO: 27; the amino acid I at a residue corresponding to position 262 in SEQ ID NO: 27; the amino acid N at a residue corresponding to position 280 in SEQ ID NO: 27; the amino acid R at a residue corresponding to position 287 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 295 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 341 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 353 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 386 in SEQ ID NO: 27; the amino acid H at a residue corresponding to position 392 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 394 in SEQ ID NO: 27; the amino acid F at a residue corresponding to position 398 in SEQ ID NO: 27; the amino acid T at a residue corresponding to position 398 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 398 in SEQ ID NO: 27; the amino acid L at a residue corresponding to position 398 in SEQ ID NO: 27; the amino acid N at a residue corresponding to position 410 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 423 in SEQ ID NO: 27; the amino acid Y at a residue corresponding to position 426 in SEQ ID NO: 27; the amino acid P at a residue corresponding to position 446 in SEQ ID NO: 27; the amino acid K at a residue corresponding to position 450 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 456 in SEQ ID NO: 27; the amino acid W at a residue corresponding to position 458 in SEQ ID NO: 27; the amino acid N at a residue corresponding to position 466 in SEQ ID NO: 27; the amino acid S at a residue corresponding to position 469 in SEQ ID NO: 27; the amino acid R at a residue corresponding to position 472 in SEQ ID NO: 27; the amino acid A at a residue corresponding to position 472 in SEQ ID NO: 27; and/or the amino acid K at a residue corresponding to position 450 in SEQ ID NO: 27. 
     In some embodiments, the TS comprises one or more of the following amino acid substitutions relative to SEQ ID NO: 27: V25A; T33D; D35A Y39F; L43I; T55S; A57Q; A57E; G61A; V62I; V63I; Y71I; T102N; T102Q; T102S; E112V; E112T; V114T; N122S; N122G; N122A; N122E; I126A; I126R; I126T; I126K; I126D; Y129W; N131S; Q161K; S180T; R183T; N202S; N202G; Y256F; Y256M; N257S; V260M; V260F; F262I; D280N; H287R; N295S; A341S; H353A; V386A; L392H; M394T; V398F; V398T; V398A; V398L; D410N; S423A; H426Y; T446P; R450K; E456A; L458W; H466N; G469S; P472R; P472A; and/or R450K. 
     Residues Y256, L392, and M394 of SEQ ID NO: 27, which are all large, hydrophobic amino acids, are predicted to be located within the active site. Without wishing to be bound by any theory, mutations at these positions may shift the product profile toward CBCA and away from CBDA at least in part by physically blocking the folding of CBGA in a manner that sterically prevents CBDA synthesis. 
     In some embodiments, one or more amino acid substitutions increases the product specificity of the TS, such as the specificity for a compound of Formula (11), CBCA, CBCVA or a combination thereof, as compared to a TS without such substitution. In some embodiments, the one or more amino acid substitutions include: A57Q and G61A; V260M; V62I; V386A; V260F; E112V and N122S; A57E and I126A; T33D and N257S; N202S and P472A; D410N; R450K; S180T; R183T; N122G and I126R; N122A and I126T; Y71I; H287R and A341S; T55S and I126T; N122G and V398F; M394T; A57E; N131S; V63I; N122G and I126R; P472R; S180T; V398A; R183T; V260M; V386A; H426Y; Y256M; N202S and P472A; N122G and I126K; V62I; R450K; Y129W; S423A; H287R and A341S; N295S; Y39F; V260F; L392H; A57E and N131S; E112V and N122S; T33D and N257S. 
     In some embodiments, the one or more amino acid substitutions include: A57Q and G61A; Y71I; and/or V260F. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Mutations in  A. niger  CBCAS that 
               
               
                 demonstrated increased CBCA titer 
               
            
           
           
               
               
               
               
            
               
                   
                 Residue in 
                   
                   
               
               
                   
                 SEQ ID NO: 27 
                 Amino Acid Substitutions 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 T33 
                 D 
                 — 
                 — 
                 — 
               
               
                   
                 Y39 
                 F 
                 — 
                 — 
                 — 
               
               
                   
                 T55 
                 S 
                 — 
                 — 
                 — 
               
               
                   
                 A57 
                 Q 
                 E 
                 — 
                 — 
               
               
                   
                 G61 
                 A 
                 — 
                 — 
                 — 
               
               
                   
                 V62 
                 I 
                 — 
                 — 
                 — 
               
               
                   
                 V63 
                 I 
                 — 
                 — 
                 — 
               
               
                   
                 Y71 
                 I 
                 — 
                 — 
                 — 
               
               
                   
                 E112 
                 V 
                 — 
                 — 
                 — 
               
               
                   
                 N122 
                 S 
                 G 
                 A 
                 — 
               
               
                   
                 I126 
                 A 
                 R 
                 T 
                 K 
               
               
                   
                 Y129 
                 W 
                 — 
                 — 
                 — 
               
               
                   
                 N131 
                 S 
                 — 
                 — 
                 — 
               
               
                   
                 S180 
                 T 
                 — 
                 — 
                 — 
               
               
                   
                 R183 
                 T 
                 — 
                 — 
                 — 
               
               
                   
                 N202 
                 S 
                 — 
                 — 
                 — 
               
               
                   
                 Y256 
                 M 
                 — 
                 — 
                 — 
               
               
                   
                 N257 
                 S 
                 — 
                 — 
                 — 
               
               
                   
                 V260 
                 M 
                 F 
                 — 
                 — 
               
               
                   
                 H287 
                 R 
                 — 
                 — 
                 — 
               
               
                   
                 N295 
                 S 
                 — 
                 — 
                 — 
               
               
                   
                 A341 
                 S 
                 — 
                 — 
                 — 
               
               
                   
                 V386 
                 A 
                 — 
                 — 
                 — 
               
               
                   
                 L392 
                 H 
                 — 
                 — 
                 — 
               
               
                   
                 M394 
                 T 
                 — 
                 — 
                 — 
               
               
                   
                 V398 
                 F 
                 A 
                 — 
                 — 
               
               
                   
                 D410 
                 N 
                 — 
                 — 
                 — 
               
               
                   
                 S423 
                 A 
                 — 
                 — 
                 — 
               
               
                   
                 H426 
                 Y 
                 — 
                 — 
                 — 
               
               
                   
                 R450 
                 K 
                 — 
                 — 
                 — 
               
               
                   
                 P472 
                 A 
                 R 
                 — 
                 — 
               
               
                   
                   
               
            
           
         
       
     
     Additional Cannabinoid Pathway Enzymes 
     Methods for production of cannabinoids and cannabinoid precursors can further include expression of one or more of: an acyl activating anzyme (AAE); a polyketide synthase (PKS) (e.g., OLS); a polykeide cyclase (PKC); and a prenyltransferase (PT). 
     Acyl Activating Enzyme (AAE) 
     A host cell described in this disclosure may comprise an AAE. As used in this disclosure, an AAE refers to an enzyme that is capable of catalyzing the esterification between a thiol and a substrate (e.g., optionally substituted aliphatic or aryl group) that has a carboxylic acid moiety. In some embodiments, an AAE is capable of using Formula (1): 
     
       
         
         
             
             
         
       
     
     or a salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative thereof to produce a product of Formula (2): 
     
       
         
         
             
             
         
       
     
     R is as defined in this application. In certain embodiments, R is hydrogen. In certain embodiments, R is optionally substituted alkyl. In certain embodiments, R is optionally substituted C1-40 alkyl. In certain embodiments, R is optionally substituted C2-40 alkyl. In certain embodiments, R is optionally substituted C2-40 alkyl, which is straight chain or branched alkyl. In certain embodiments, R is optionally substituted C2-10 alkyl, optionally substituted C10-C20 alkyl, optionally substituted C20-C30 alkyl, optionally substituted C30-C40 alkyl, or optionally substituted C40-C50 alkyl, which is straight chain or branched alkyl. In certain embodiments, R is optionally substituted C3-8 alkyl. In certain embodiments, R is optionally substituted C1-C40 alkyl, C1-C20 alkyl, C1-C10 alkyl, C1-C8 alkyl, C1-C5 alkyl, C3-C5 alkyl, C3 alkyl, or C5 alkyl. In certain embodiments, R is optionally substituted C1-C20 alkyl. In certain embodiments, R is optionally substituted C1-C20 branched alkyl. In certain embodiments, R is optionally substituted C1-C20 alkyl, optionally substituted C1-C10 alkyl, optionally substituted C10-C20 alkyl, optionally substituted C20-C30 alkyl, optionally substituted C30-C40 alkyl, or optionally substituted C40-C50 alkyl. In certain embodiments, R is optionally substituted C1-C10 alkyl. In certain embodiments, R is optionally substituted C3 alkyl. In certain embodiments, R is optionally substituted n-propyl. In certain embodiments, R is unsubstituted n-propyl. In certain embodiments, R is optionally substituted C1-C8 alkyl. In some embodiments, R is a C2-C6 alkyl. In certain embodiments, R is optionally substituted C1-C5 alkyl. In certain embodiments, R is optionally substituted C3-C5 alkyl. In certain embodiments, R is optionally substituted C3 alkyl. In certain embodiments, R is optionally substituted C5 alkyl. In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is optionally substituted propyl. In certain embodiments, R is optionally substituted n-propyl. In certain embodiments, R is n-propyl optionally substituted with optionally substituted aryl. In certain embodiments, R is n-propyl optionally substituted with optionally substituted phenyl. In certain embodiments, R is n-propyl substituted with unsubstituted phenyl. In certain embodiments, R is optionally substituted butyl. In certain embodiments, R is optionally substituted n-butyl. In certain embodiments, R is n-butyl optionally substituted with optionally substituted aryl. In certain embodiments, R is n-butyl optionally substituted with optionally substituted phenyl. In certain embodiments, R is n-butyl substituted with unsubstituted phenyl. In certain embodiments, R is optionally substituted pentyl. In certain embodiments, R is optionally substituted n-pentyl. In certain embodiments, R is n-pentyl optionally substituted with optionally substituted aryl. In certain embodiments, R is n-pentyl optionally substituted with optionally substituted phenyl. In certain embodiments, R is n-pentyl substituted with unsubstituted phenyl. In certain embodiments, R is optionally substituted hexyl. In certain embodiments, R is optionally substituted n-hexyl. In certain embodiments, R is optionally substituted n-heptyl. In certain embodiments, R is optionally substituted n-octyl. In certain embodiments, R is alkyl optionally substituted with aryl (e.g., phenyl). In certain embodiments, R is optionally substituted acyl (e.g., —C(═O)Me). 
     In certain embodiments, R is optionally substituted alkenyl (e.g., substituted or unsubstituted C 2-6  alkenyl). In certain embodiments, R is substituted or unsubstituted C 2-6  alkenyl. In certain embodiments, R is substituted or unsubstituted C 2-5  alkenyl. In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is optionally substituted alkynyl (e.g., substituted or unsubstituted C 2-6  alkynyl). In certain embodiments, R is substituted or unsubstituted C 2-6  alkynyl. In certain embodiments, R is of formula: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R is optionally substituted carbocyclyl. In certain embodiments, R is optionally substituted aryl (e.g., phenyl or napthyl). 
     In some embodiments, a substrate for an AAE is produced by fatty acid metabolism within a host cell. In some embodiments, a substrate for an AAE is provided exogenously. 
     In some embodiments, an AAE is capable of catalyzing the formation of hexanoyl-coenzyme A (hexanoyl-CoA) from hexanoic acid and coenzyme A (CoA). In some embodiments, an AAE is capable of catalyzing the formation of butanoyl-coenzyme A (butanoyl-CoA) from butanoic acid and coenzyme A (CoA). 
     As one of ordinary skill in the art would appreciate, an AAE could be obtained from any source, including naturally occurring sources and synthetic sources (e.g., a non-naturally occurring AAE). In some embodiments, an AAE is a  Cannabis  enzyme. Non-limiting examples of AAEs include  C. sativa  hexanoyl-CoA synthetase 1 (CsHCS1) and  C. sativa  hexanoyl-CoA synthetase 2 (CsHCS2) as disclosed in U.S. Pat. No. 9,546,362, which is incorporated by reference in this application in its entirety. 
     CsHCS1 has the sequence: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 5) 
               
               
                 MGKNYKSLDSVVASDFIALGITSEVAETLHGRLAEIVCNYGAATPQTWIN 
               
               
                   
               
               
                 IANHILSPDLPFSLHQMLFYGCYKDFGPAPPAWIPDPEKVKSTNLGALLE 
               
               
                   
               
               
                 KRGKEFLGVKYKDPISSFSHFQEFSVRNPEVYWRTVLMDEMKISFSKDPE 
               
               
                   
               
               
                 CILRRDDINNPGGSEWLPGGYLNSAKNCLNVNSNKKLNDTMIVWRDEGND 
               
               
                   
               
               
                 DLPLNKLTLDQLRKRVWLVGYALEEMGLEKGCAIAIDMPMHVDAVVIYLA 
               
               
                   
               
               
                 IVLAGYVVVSIADSFSAPEISTRLRLSKAKAIFTQDHIIRGKKRIPLYSR 
               
               
                   
               
               
                 VVEAKSPMAIVIPCSGSNIGAELRDGDISWDYFLERAKEFKNCEFTAREQ 
               
               
                   
               
               
                 PVDAYTNILFSSGTTGEPKAIPWTQATPLKAAADGWSHLDIRKGDVIVWP 
               
               
                   
               
               
                 TNLGWMMGPWLVYASLLNGASIALYNGSPLVSGFAKFVQDAKVTMLGVVP 
               
               
                   
               
               
                 SIVRSWKSTNCVSGYDWSTIRCFSSSGEASNVDEYLWLMGRANYKPVIEM 
               
               
                   
               
               
                 CGGTEIGGAFSAGSFLQAQSLSSFSSQCMGCTLYILDKNGYPMPKNKPGI 
               
               
                   
               
               
                 GELALGPVMFGASKTLLNGNHHDVYFKGMPTLNGEVLRRHGDIFELTSNG 
               
               
                   
               
               
                 YYHAHGRADDTMNIGGIKISSIEIERVCNEVDDRVFETTAIGVPPLGGGP 
               
               
                   
               
               
                 EQLVIFFVLKDSNDTTIDLNQLRLSFNLGLQKKLNPLFKVTRVVPLSSLP 
               
               
                   
               
               
                 RTATNKIMRRVLRQFSHFE. 
               
            
           
         
       
     
     CsHCS2 has the sequence: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 6) 
               
               
                 MEKSGYGRDGIYRSLRPPLHLPNNNNLSMVSFLFRNSSSYPQKPALIDSE 
               
               
                   
               
               
                 TNQILSFSHFKSTVIKVSHGFLNLGIKKNDVVLIYAPNSIHFPVCFLGII 
               
               
                   
               
               
                 ASGAIATTSNPLYTVSELSKQVKDSNPKLIITVPQLLEKVKGFNLPTILI 
               
               
                   
               
               
                 GPDSEQESSSDKVMTFNDLVNLGGSSGSEFPIVDDFKQSDTAALLYSSGT 
               
               
                   
               
               
                 TGMSKGVVLTHKNFIASSLMVTMEQDLVGEMDNVFLCFLPMFHVFGLAII 
               
               
                   
               
               
                 TYAQLQRGNTVISMARFDLEKMLKDVEKYKVTHLWVVPPVILALSKNSMV 
               
               
                   
               
               
                 KKFNLSSIKYIGSGAAPLGKDLMEECSKVVPYGIVAQGYGMTETCGIVSM 
               
               
                   
               
               
                 EDIRGGKRNSGSAGMLASGVEAQIVSVDTLKPLPPNQLGEIWVKGPNMMQ 
               
               
                   
               
               
                 GYFNNPQATKLTIDKKGWVHTGDLGYFDEDGHLYVVDRIKELIKYKGFQV 
               
               
                   
               
               
                 APAELEGLLVSHPEILDAVVIPFPDAEAGEVPVAYVVRSPNSSLTENDVK 
               
               
                   
               
               
                 KFIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQKVRSNM. 
               
            
           
         
       
     
     Polyketide Synthases (PKS) 
     A host cell described in this application may comprise a PKS. As used in this application, a “PKS” refers to an enzyme that is capable of producing a polyketide. In certain embodiments, a PKS converts a compound of Formula (2) to a compound of Formula (4), (5), and/or (6). In certain embodiments, a PKS converts a compound of Formula (2) to a compound of Formula (4). In certain embodiments, a PKS converts a compound of Formula (2) to a compound of Formula (5). In certain embodiments, a PKS converts a compound of Formula (2) to a compound of Formula (4) and/or (5). In certain embodiments, a PKS converts a compound of Formula (2) to a compound of Formula (5) and/or (6). 
     In some embodiments, a PKS is a tetraketide synthase (TKS). In certain embodiments, a PKS is an olivetol synthase (OLS). As used in this application, an “OLS” refers to an enzyme that is capable of using a substrate of Formula (2a) to form a compound of Formula (4a), (5a) or (6a) as shown in  FIG.  1   . 
     In certain embodiments, a PKS is a divarinic acid synthase (DVS). 
     In certain embodiments, polyketide synthases can use hexanoyl-CoA or any acyl-CoA (or a product of Formula (2): 
     
       
         
         
             
             
         
       
     
     and three malonyl-CoAs as substrates to form 3,5,7-trioxododecanoyl-CoA or other 3,5,7-trioxo-acyl-CoA derivatives; or to form a compound of Formula (4): 
     
       
         
         
             
             
         
       
     
     wherein R is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; depending on substrate. R is as defined in this application. In some embodiments, R is a C2-C6 optionally substituted alkyl. In some embodiments, R is a propyl or pentyl. In some embodiments, R is pentyl. In some embodiments, R is propyl. A PKS may also bind isovaleryl-CoA, octanoyl-CoA, hexanoyl-CoA, and butyryl-CoA. In some embodiments, a PKS is capable of catalyzing the formation of a 3,5,7-trioxoalkanoyl-CoA (e.g. 3,5,7-trioxododecanoyl-CoA). In some embodiments, an OLS is capable of catalyzing the formation of a 3,5,7-trioxoalkanoyl-CoA (e.g. 3,5,7-trioxododecanoyl-CoA). 
     In some embodiments, a PKS uses a substrate of Formula (2) to form a compound of Formula (4): 
     
       
         
         
             
             
         
       
     
     wherein R is unsubstituted pentyl. 
     As one of ordinary skill in the art would appreciate a PKS, such as an OLS, could be obtained from any source, including naturally occurring sources and synthetic sources (e.g., a non-naturally occurring PKS). In some embodiments a PKS is from  Cannabis . In some embodiments a PKS is from Dictyostelium. Non-limiting examples of PKS enzymes may be found in U.S. Pat. No. 6,265,633; WO 2018/148848 A1; WO 2018/148849 A1; and US 2018/155748, which are incorporated by reference in this application in their entireties. 
     A non-limiting example of an OLS is provided by UniProtKB—B1Q2B6 from  C. sativa . In  C. sativa , this OLS uses hexanoyl-CoA and malonyl-CoA as substrates to form 3,5,7-trioxododecanoyl-CoA. OLS (e.g., UniProtKB—B1Q2B6) in combination with olivetolic acid cyclase (OAC) produces olivetolic acid (OA) in  C. sativa.    
     The amino acid sequence of UniProtKB—B1Q2B6 is: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 7) 
               
               
                 MNHLRAEGPASVLAIGTANPENILLQDEFPDYYFRVTKSEHMTQLKEKFR 
               
               
                   
               
               
                 KICDKSMIRKRNCFLNEEHLKQNPRLVEHEMQTLDARQDMLVVEVPKLGK 
               
               
                   
               
               
                 DACAKAIKEWGQPKSKITHLIFTSASTTDMPGADYHCAKLLGLSPSVKRV 
               
               
                   
               
               
                 MMYQLGCYGGGTVLRIAKDIAENNKGARVLAVCCDIMACLFRGPSESDLE 
               
               
                   
               
               
                 LLVGQAIFGDGAAAVIVGAEPDESVGERPIFELVSTGQTILPNSEGTIGG 
               
               
                   
               
               
                 HIREAGLIFDLHKDVPMLISNNIEKCLIEAFTPIGISDWNSIFWITHPGG 
               
               
                   
               
               
                 KAILDKVEEKLHLKSDKFVDSRHVLSEHGNMSSSTVLFVMDELRKRSLEE 
               
               
                   
               
               
                 GKSTTGDGFEWGVLFGFGPGLTVERVVVRSVPIKY. 
               
            
           
         
       
     
     PKS enzymes described in this application may or may not have cyclase activity. In some embodiments where the PKS enzyme does not have cyclase activity, one or more exogenous polynucleotides that encode a polyketide cyclase (PKC) enzyme may also be co-expressed in the same host cells to enable conversion of hexanoic acid or butyric acid or other fatty acid conversion into olivetolic acid or divarinolic acid or other precursors of cannabinoids. In some embodiments, the PKS enzyme and a PKC enzyme are expressed as separate distinct enzymes. In some embodiments, a PKS enzyme that lacks cyclase activity and a PKC are linked as part of a fusion polypeptide that is a bifunctional PKS. In some embodiments, a bifunctional PKC is referred to as a bifunctional PKS-PKC. In some embodiments, a bifunctional PKC is a bifunctional tetraketide synthase (TKS-TKC). As used in this application, a bifunctional PKS is an enzyme that is capable of producing a compound of Formula (6): 
     
       
         
         
             
             
         
       
     
     from a compound of Formula (2): 
     
       
         
         
             
             
         
       
     
     and a compound of Formula (3): 
     
       
         
         
             
             
         
       
     
     In some embodiments, a PKS produces more of a compound of Formula (6): 
     
       
         
         
             
             
         
       
     
     as compared to a compound of Formula (5): 
     
       
         
         
             
             
         
       
     
     As a non-limiting example, a compound of Formula (6): 
     
       
         
         
             
             
         
       
     
     is olivetolic acid (Formula (6a)): 
     
       
         
         
             
             
         
       
     
     As a non-limiting example, a compound of Formula (5): 
     
       
         
         
             
             
         
       
     
     is olivetol (Formula (5a)): 
     
       
         
         
             
             
         
       
     
     In some embodiments, a polyketide synthase of the present disclosure is capable of catalyzing a compound of Formula (2): 
     
       
         
         
             
             
         
       
     
     and a compound of Formula (3): 
     
       
         
         
             
             
         
       
     
     to produce a compound of Formula (4): 
     
       
         
         
             
             
         
       
     
     and also further catalyzes a compound of Formula (4): 
     
       
         
         
             
             
         
       
     
     to produce a compound of Formula (6): 
     
       
         
         
             
             
         
       
     
     In some embodiments, the PKS is not a fusion protein. In some embodiments, a PKS that is capable of catalyzing a compound of Formula (2): 
     
       
         
         
             
             
         
       
     
     and a compound of Formula (3): 
     
       
         
         
             
             
         
       
     
     to produce a compound of Formula (4): 
     
       
         
         
             
             
         
       
     
     and is also capable of further catalyzing the production of a compound of Formula (6): 
     
       
         
         
             
             
         
       
     
     from the compound of Formula (4): 
     
       
         
         
             
             
         
       
     
     is preferred because it avoids the need for an additional polyketide cyclase to produce a compound of Formula (6): 
     
       
         
         
             
             
         
       
     
     In some embodiments, such an enzyme that is a bifunctional PKS eliminates the transport considerations needed with addition of a polyketide cyclase, whereby the compound of Formula (4), being the product of the PKS, must be transported to the PKS for use as a substrate to be converted into the compound of Formula (6). 
     In some embodiments, a PKS is capable of producing olivetolic acid in the presence of a compound of Formula (2a): 
     
       
         
         
             
             
         
       
     
     and Formula (3a): 
     
       
         
         
             
             
         
       
     
     In some embodiments, an OLS is capable of producing olivetolic acid in the presence of a compound of Formula (2a): 
     
       
         
         
             
             
         
       
     
     and Formula (3a): 
     
       
         
         
             
             
         
       
     
     Polyketide Cyclase (PKC) 
     A host cell described in this disclosure may comprise a PKC. As used in this application, a “PKC” refers to an enzyme that is capable of cyclizing a polyketide. 
     In certain embodiments, a polyketide cyclase (PKC) catalyzes the cyclization of an oxo fatty acyl-CoA (e.g., a compound of Formula (4): 
     
       
         
         
             
             
         
       
     
     or 3,5,7-trioxododecanoyl-COA, 3,5,7-trioxodecanoyl-COA) to the corresponding intramolecular cyclization product (e.g., compound of Formula (6), including olivetolic acid and divarinic acid). In some embodiments, a PKC catalyzes the formation of a compound which occurs in the presence of a PKS. PKC substrates include trioxoalkanol-CoA, such as 3,5,7-Trioxododecanoyl-CoA, or a compound of Formula (4): 
     
       
         
         
             
             
         
       
     
     wherein R is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl. In certain embodiments, a PKC catalyzes a compound of Formula (4): 
     
       
         
         
             
             
         
       
     
     wherein R is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; to form a compound of Formula (6): 
     
       
         
         
             
             
         
       
     
     wherein R is hydrogen, optionally substituted acyl, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, or optionally substituted aryl; as substrates. R is as defined in this application. In some embodiments, R is a C2-C6 optionally substituted alkyl. In some embodiments, R is a propyl or pentyl. In some embodiments, R is pentyl. In some embodiments, R is propyl. In certain embodiments, a PKC is an olivetolic acid cyclase (OAC). In certain embodiments, a PKC is a divarinic acid cyclase (DAC). 
     As one of ordinary skill in the art would appreciate a PKC could be obtained from any source, including naturally occurring sources and synthetic sources (e.g., a non-naturally occurring PKC). In some embodiments, a PKC is from  Cannabis . Non-limiting examples of PKCs include those disclosed in U.S. Pat. Nos. 9,611,460; 10,059,971; and U.S. Patent No. 2019/0169661, which are incorporated by reference in this application in their entireties. 
     In some embodiments, a PKC is an OAC. As used in this application, an “OAC” refers to an enzyme that is capable of catalyzing the formation of olivetolic acid (OA). In some embodiments, an OAC is an enzyme that is capable of using a substrate of Formula (4a) (3,5,7-trioxododecanoyl-CoA): 
     
       
         
         
             
             
         
       
     
     to form a compound of Formula (6a) (olivetolic acid): 
     
       
         
         
             
             
         
       
     
     Olivetolic acid cyclase from  C. sativa  (CsOAC) is a 101 amino acid enzyme that performs non-decaboxylative cyclization of the tetraketide product of olivetol synthase ( FIG.  4    Structure 4a) via aldol condensation to form olivetolic acid ( FIG.  4    Structure 6a). CsOAC was identified and characterized by Gagne et al. (PNAS 2012) via transcriptome mining, and its cyclization function was recapitulated in vitro to demonstrate that CsOAC is required for formation of olivetolic acid in  C. sativa . A crystal structure of the enzyme was published by Yang et al. (FEBS J. 2016 March; 283(6):1088-106), which revealed that the enzyme is a homodimer and belongs to the α+β barrel (DABB) superfamily of protein folds. CsOAC is the only known plant polyketide cyclase. Multiple fungal Type III polyketide synthases have been identified that perform both polyketide synthase and cyclization functions (Funa et al., J Biol Chem. 2007 May 11; 282(19):14476-81); however, in plants such a dual function enzyme has not yet been discovered. 
     A non-limiting example of an amino acid sequence of an OAC in  C. sativa  is provided by UniProtKB—I6WU39 (SEQ ID NO: 1), which catalyzes the formation of olivetolic acid (OA) from 3,5,7-Trioxododecanoyl-CoA. 
     The sequence of UniProtKB—I6WU39 (SEQ ID NO: 1) is: 
     
       
         
           
               
            
               
                 MAVKHLIVLKFKDEITEAQKEEFFKTYVNLVNIIPAMKDVYWGKDVTQKN 
               
               
                   
               
               
                 KEEGYTHIVEVTFESVETIQDYIIHPAHVGFGDVYRSFWEKLLIFDYTPR 
               
               
                   
               
               
                 K. 
               
            
           
         
       
     
     A non-limiting example of a nucleic acid sequence encoding  C. sativa  OAC is: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 2) 
               
               
                 atggcagtgaagcatttgattgtattgaagttcaaagatgaaatcacaga 
               
               
                   
               
               
                 agcccaaaaggaagaatttttcaagacgtatgtgaatcttgtgaatatca 
               
               
                   
               
               
                 tcccagccatgaaagatgtatactggggtaaagatgtgactcaaaagaat 
               
               
                   
               
               
                 aaggaagaagggtacactcacatagttgaggtaacatttgagagtgtgga 
               
               
                   
               
               
                 gactattcaggactacattattcatcctgcccatgttggatttggagatg 
               
               
                   
               
               
                 tctatcgttctttctgggaaaaacttctcatttttgactacacaccacga 
               
               
                   
               
               
                 aag. 
               
            
           
         
       
     
     Prenyltransferase (PT) 
     A host cell described in this application may comprise a prenyltransferase (PT). As used in this application, a “PT” refers to an enzyme that is capable of transferring prenyl groups to acceptor molecule substrates. Non-limiting examples of prenyltransferases are described in PCT Publication No. WO2018200888 (e.g., CsPT4), U.S. Pat. No. 8,884,100 (e.g., CsPT1); Canadian Patent No. CA2718469; Valliere et al.,  Nat Commun.  2019 Feb. 4; 10(1):565; and Luo et al.,  Nature  2019 March; 567(7746):123-126, which are incorporated by reference in their entireties. In some embodiments, a PT is capable of producing cannabigerolic acid (CBGA), cannabigerovarinic acid (CBGVA), or other cannabinoids or cannabinoid-like substances. In some embodiments, a PT is cannabigerolic acid synthase (CBGAS). In some embodiments, a PT is cannabigerovarinic acid synthase (CBGVAS). 
     In some embodiments, the PT is an NphB prenyltransferase. See, e.g., U.S. Pat. No. 7,544,498; and Kumano et al.,  Bioorg Med Chem.  2008 Sep. 1; 16(17): 8117-8126, which are incorporated by reference in this application in their entireties. In some embodiments, a PT corresponds to NphB from  Streptomyces  sp. (see, e.g., UniprotKB Accession No. Q4R2T2; see also SEQ ID NO: 2 of U.S. Pat. No. 7,361,483). The protein sequence corresponding to UniprotKB Accession No. Q4R2T2 is provided by SEQ ID NO: 8: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 8) 
               
               
                 MSEAADVERVYAAMEEAAGLLGVACARDKIYPLLSTFQDTLVEGGSVVVF 
               
               
                   
               
               
                 SMASGRHSTELDFSISVPTSHGDPYATVVEKGLFPATGHPVDDLLADTQK 
               
               
                   
               
               
                 HLPVSMFAIDGEVTGGFKKTYAFFPTDNMPGVAELSAIPSMPPAVAENAE 
               
               
                   
               
               
                 LFARYGLDKVQMTSMDYKKRQVNLYFSELSAQTLEAESVLALVRELGLHV 
               
               
                   
               
               
                 PNELGLKFCKRSFSVYPTLNWETGKIDRLCFAVISNDPTLVPSSDEGDIE 
               
               
                   
               
               
                 KFHNYATKAPYAYVGEKRTLVYGLTLSPKEEYYKLGAYYHITDVQRGLLK 
               
               
                   
               
               
                 AFDSLED. 
               
            
           
         
       
     
     A non-limiting example of a nucleic acid sequence encoding NphB is: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 9) 
               
               
                 atgtcagaagccgcagatgtcgaaagagtttacgccgctatggaagaagc 
               
               
                   
               
               
                 cgccggtttgttaggtgttgcctgtgccagagataagatctacccattgt 
               
               
                   
               
               
                 tgtctacttttcaagatacattagttgaaggtggttcagttgttgttttc 
               
               
                   
               
               
                 tctatggcttcaggtagacattctacagaattggatttctctatctcagt 
               
               
                   
               
               
                 tccaacatcacatggtgatccatacgctactgttgttgaaaaaggtttat 
               
               
                   
               
               
                 ttccagcaacaggtcatccagttgatgatttgttggctgatactcaaaag 
               
               
                   
               
               
                 catttgccagtttctatgtttgcaattgatggtgaagttactggtggttt 
               
               
                   
               
               
                 caagaaaacttacgctttctttccaactgataacatgccaggtgttgcag 
               
               
                   
               
               
                 aattatctgctattccatcaatgccaccagctgttgcagaaaatgcagaa 
               
               
                   
               
               
                 ttatttgctagatacggtttggataaggttcaaatgacatctatggatta 
               
               
                   
               
               
                 caagaaaagacaagttaatttgtacttttctgaattatcagcacaaactt 
               
               
                   
               
               
                 tggaagctgaatcagttttggcattagttagagaattgggtttacatgtt 
               
               
                   
               
               
                 ccaaacgaattgggtttgaagttttgtaaaagatctttctcagtttatcc 
               
               
                   
               
               
                 aactttaaactgggaaacaggcaagatcgatagattatgtttcgcagtta 
               
               
                   
               
               
                 tctctaacgatccaacattggttccatcttcagatgaaggtgatatcgaa 
               
               
                   
               
               
                 aagtttcataactacgctactaaagcaccatatgcttacgttggtgaaaa 
               
               
                   
               
               
                 gagaacattagtttatggtttgactttatcaccaaaggaagaatactaca 
               
               
                   
               
               
                 agttgggtgcttactaccacattaccgacgtacaaagaggtttattgaaa 
               
               
                   
               
               
                 gcattcgatagtttagaagactaa. 
               
            
           
         
       
     
     In other embodiments, a PT corresponds to CsPT1, which is disclosed as SEQ ID NO:2 in U.S. Pat. No. 8,884,100 ( C. sativa ; corresponding to SEQ ID NO: 10 in this application): 
     
       
         
           
               
            
               
                 (SEQ ID NO: 10) 
               
               
                 MGLSSVCTFSFQTNYHTLLNPHNNNPKTSLLCYRHPKTPIKYSYNNFPSK 
               
               
                   
               
               
                 HCSTKSFHLQNKCSESLSIAKNSIRAATTNQTEPPESDNHSVATKILNFG 
               
               
                   
               
               
                 KACWKLQRPYTIIAFTSCACGLFGKELLHNTNLISWSLMFKAFFFLVAIL 
               
               
                   
               
               
                 CIASFTTTINQIYDLHIDRINKPDLPLASGEISVNTAWIMSIIVALFGLI 
               
               
                   
               
               
                 ITIKMKGGPLYIFGYCFGIFGGIVYSVPPFRWKQNPSTAFLLNFLAHIIT 
               
               
                   
               
               
                 NFTFYYASRAALGLPFELRPSFTFLLAFMKSMGSALALIKDASDVEGDTK 
               
               
                   
               
               
                 FGISTLASKYGSRNLTLFCSGIVLLSYVAAILAGIIWPQAFNSNVMLLSH 
               
               
                   
               
               
                 AILAFWLILQTRDFALTNYDPEAGRRFYEFMWKLYYAEYLVYVFI. 
               
            
           
         
       
     
     In some embodiments, a PT corresponds to CsPT4, which is disclosed as SEQ ID NO:1 in PCT Publication No. WO2019071000, corresponding to SEQ ID NO: 11 in this application: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 11) 
               
               
                 MGLSLVCTFSFQTNYHTLLNPHNKNPKNSLLSYQHPKTPIIKSSYDNFPS 
               
               
                   
               
               
                 KYCLTKNFHLLGLNSHNRISSQSRSIRAGSDQIEGSPHHESDNSIATKIL 
               
               
                   
               
               
                 NFGHTCWKLQRPYVVKGMISIACGLFGRELFNNRHLFSWGLMWKAFFALV 
               
               
                   
               
               
                 PILSFNFFAAIMNQIYDVDIDRINKPDLPLVSGEMSIETAWILSIIVALT 
               
               
                   
               
               
                 GLIVTIKLKSAPLFVFIYIFGIFAGFAYSVPPIRWKQYPFTNFLITISSH 
               
               
                   
               
               
                 VGLAFTSYSATTSALGLPFVWRPAFSFIIAFMTVMGMTIAFAKDISDIEG 
               
               
                   
               
               
                 DAKYGVSTVATKLGARNMTFVVSGVLLLNYLVSISIGIIWPQVFKSNIMI 
               
               
                   
               
               
                 LSHAILAFCLIFQTRELALANYASAPSRQFFEFIWLLYYAEYFVYVFI. 
               
            
           
         
       
     
     In some embodiments, a PT corresponds to a truncated CsPT4, which is provided as SEQ ID NO: 12: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 12) 
               
               
                 MSAGSDQIEGSPHHESDNSIATKILNFGHTCWKLQRPYVVKGMISIACGL 
               
               
                   
               
               
                 FGRELFNNRHLFSWGLMWKAFFALVPILSFNFFAAIMNQIYDVDIDRINK 
               
               
                   
               
               
                 PDLPLVSGEMSIETAWILSIIVALTGLIVTIKLKSAPLFVFIYIFGIFAG 
               
               
                   
               
               
                 FAYSVPPIRWKQYPFTNFLITISSHVGLAFTSYSATTSALGLPFVWRPAF 
               
               
                   
               
               
                 SFIIAFMTVMGMTIAFAKDISDIEGDAKYGVSTVATKLGARNMTFVVSGV 
               
               
                   
               
               
                 LLLNYLVSISIGIIWPQVFKSNIMILSHAILAFCLIFQTRELALANYASA 
               
               
                   
               
               
                 PSRQFFEFIWLLYYAEYFVYVFI. 
               
            
           
         
       
     
     Functional expression of paralog  C. sativa  CBGAS enzymes in  S. cerevisiae  and production of the major cannabinoid CBGA has been reported (U.S. Patent Publication 2012/0144523, and Luo et al. Nature, 2019 March; 567(7746):123-126). Luo et al. reported the production of CBGA in  S. cerevisiae  by expressing a truncated version of a  C. sativa  CBGAS, CsPT4, with its native signal peptide removed. Without being bound by a particular theory, the integral-membrane nature of  C. sativa  CBGAS enzymes may render functional expression of  C. sativa  CBGAS enzymes in heterologous hosts challenging. Removal of transmembrane domain(s) or signal sequences or use of prenyltransferases that are not associated with the membrane and are not integral membrane proteins may facilitate increased interaction between the enzyme and available substrate, for example in the cellular cytosol and/or in organelles that may be targeted using peptides that confer localization. 
     In some embodiments, the PT is a soluble PT. In some embodiments, the PT is a cytosolic PT. In some embodiments, the PT is a secreted protein. In some embodiments, the PT is not a membrane-associated protein. In some embodiments, the PT is not an integral membrane protein. In some embodiments, the PT does not comprise a transmembrane domain or a predicted transmembrane. In some embodiments, the PT may be primarily detected in the cytosol (e.g., detected in the cytosol to a greater extent than detected associated with the cell membrane). In some embodiments, the PT is a protein from which one or more transmembrane domains have been removed and/or mutated (e.g., by truncation, deletions, substitutions, insertions, and/or additions) so that the PT localizes or is predicted to localize in the cytosol of the host cell, or to cytosolic organelles within the host cell, or, in the case of bacterial hosts, in the periplasm. In some embodiments, the PT is a protein from which one or more transmembrane domains have been removed or mutated (e.g., by truncation, deletions, substitutions, insertions, and/or additions) so that the PT has increased localization to the cytosol, organelles, or periplasm of the host cell, as compared to membrane localization. 
     Within the scope of the term “transmembrane domains” are predicted or putative transmembrane domains in addition to transmembrane domains that have been empirically determined. In general, transmembrane domains are characterized by a region of hydrophobicity that facilitates integration into the cell membrane. Methods of predicting whether a protein is a membrane protein or a membrane-associated protein are known in the art and may include, for example amino acid sequence analysis, hydropathy plots, and/or protein localization assays. 
     In some embodiments, the PT is a protein from which a signal sequence has been removed and/or mutated so that the PT is not directed to the cellular secretory pathway. In some embodiments, the PT is a protein from which a signal sequence has been removed and/or mutated so that the PT is localized to the cytosol or has increased localization to the cytosol (e.g., as compared to the secretory pathway). 
     In some embodiments, the PT is a secreted protein. In some embodiments, the PT contains a signal sequence. 
     In some embodiments, a PT is a fusion protein. For example, a PT may be fused to one or more genes in the metabolic pathway of a host cell. In certain embodiments, a PT may be fused to mutant forms of one or more genes in the metabolic pathway of a host cell. 
     In some embodiments, a PT described in this application transfers one or more prenyl groups to any of positions 1, 2, 3, 4, or 5 in a compound of Formula (6), shown below: 
     
       
         
         
             
             
         
       
     
     In some embodiments, the PT transfers a prenyl group to any of positions 1, 2, 3, 4, or 5 in a compound of Formula (6), shown below: 
     
       
         
         
             
             
         
       
     
     to form a compound of one or more of Formula (8w), Formula (8x), Formula (8′), Formula (8y), Formula (8z): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, wherein a is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10. 
     Variants 
     Aspects of the disclosure relate to nucleic acids encoding any of the polypeptides (e.g., AAE, PKS, PKC, PT, or TS) described in this application. In some embodiments, a nucleic acid encompassed by the disclosure is a nucleic acid that hybridizes under high or medium stringency conditions to a nucleic acid encoding an AAE, PKS, PKC, PT, or TS and is biologically active. For example, high stringency conditions of 0.2 to 1×SSC at 65° C. followed by a wash at 0.2×SSC at 65° C. can be used. In some embodiments, a nucleic acid encompassed by the disclosure is a nucleic acid that hybridizes under low stringency conditions to a nucleic acid encoding an AAE, PKS, PKC, PT, or TS and is biologically active. For example, low stringency conditions of 6×SSC at room temperature followed by a wash at 2×SSC at room temperature can be used. Other hybridization conditions include 3×SSC at 40 or 50° C., followed by a wash in 1 or 2×SSC at 20, 30, 40, 50, 60, or 65° C. 
     Hybridizations can be conducted in the presence of formaldehyde, e.g., 10%, 20%, 30% 40% or 50%, which further increases the stringency of hybridization. Theory and practice of nucleic acid hybridization is described, e.g., in S. Agrawal (ed.) Methods in Molecular Biology, volume 20; and Tijssen (1993) Laboratory Techniques in biochemistry and molecular biology-hybridization with nucleic acid probes, e.g., part I chapter 2 “Overview of principles of hybridization and the strategy of nucleic acid probe assays,” Elsevier, New York provide a basic guide to nucleic acid hybridization. 
     Variants of enzyme sequences described in this application (e.g., AAE, PKS, PKC, PT, or TS, including nucleic acid or amino acid sequences) are also encompassed by the present disclosure. A variant may share at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with a reference sequence, including all values in between. 
     Unless otherwise noted, the term “sequence identity,” which is used interchangeably in this disclosure with the term “percent identity,” as known in the art, refers to a relationship between the sequences of two polypeptides or polynucleotides, as determined by sequence comparison (alignment). In some embodiments, sequence identity is determined across the entire length of a sequence (e.g., AAE, PKS, PKC, PT, or TS sequence). In some embodiments, sequence identity is determined over a region (e.g., a stretch of amino acids or nucleic acids, e.g., the sequence spanning an active site) of a sequence (e.g., AAE, PKS, PKC, PT, or TS sequence). For example, in some embodiments, sequence identity is determined over a region corresponding to at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or over 100% of the length of the reference sequence. 
     Identity measures the percent of identical matches between the smaller of two or more sequences with gap alignments (if any) addressed by a particular mathematical model, algorithm, or computer program. 
     Identity of related polypeptides or nucleic acid sequences can be readily calculated by any of the methods known to one of ordinary skill in the art. The percent identity of two sequences (e.g., nucleic acid or amino acid sequences) may, for example, be determined using the algorithm of Karlin and Altschul  Proc. Natl. Acad. Sci. USA  87:2264-68, 1990, modified as in Karlin and Altschul  Proc. Natl. Acad. Sci. USA  90:5873-77, 1993. Such an algorithm is incorporated into the NBLAST® and XBLAST® programs (version 2.0) of Altschul et al.,  J. Mol. Biol.  215:403-10, 1990. BLAST® protein searches can be performed, for example, with the XBLAST program, score=50, wordlength=3 to obtain amino acid sequences homologous to the proteins described in this application. Where gaps exist between two sequences, Gapped BLAST® can be utilized, for example, as described in Altschul et al.,  Nucleic Acids Res.  25(17):3389-3402, 1997. When utilizing BLAST® and Gapped BLAST® programs, the default parameters of the respective programs (e.g., XBLAST® and NBLAST®) can be used, or the parameters can be adjusted appropriately as would be understood by one of ordinary skill in the art. 
     Another local alignment technique which may be used, for example, is based on the Smith-Waterman algorithm (Smith, T. F. &amp; Waterman, M. S. (1981) “Identification of common molecular subsequences.”  J. Mol. Biol.  147:195-197). A general global alignment technique which may be used, for example, is the Needleman-Wunsch algorithm (Needleman, S. B. &amp; Wunsch, C. D. (1970) “A general method applicable to the search for similarities in the amino acid sequences of two proteins.”  J. Mol. Biol.  48:443-453), which is based on dynamic programming. 
     More recently, a Fast Optimal Global Sequence Alignment Algorithm (FOGSAA) was developed that purportedly produces global alignment of nucleic acid and amino acid sequences faster than other optimal global alignment methods, including the Needleman-Wunsch algorithm. In some embodiments, the identity of two polypeptides is determined by aligning the two amino acid sequences, calculating the number of identical amino acids, and dividing by the length of one of the amino acid sequences. In some embodiments, the identity of two nucleic acids is determined by aligning the two nucleotide sequences and calculating the number of identical nucleotide and dividing by the length of one of the nucleic acids. 
     For multiple sequence alignments, computer programs including Clustal Omega (Sievers et al.,  Mol Syst Biol.  2011 Oct. 11; 7:539) may be used. 
     In preferred embodiments, a sequence, including a nucleic acid or amino acid sequence, is found to have a specified percent identity to a reference sequence, such as a sequence disclosed in this application and/or recited in the claims when sequence identity is determined using the algorithm of Karlin and Altschul  Proc. Natl. Acad. Sci. USA  87:2264-68, 1990, modified as in Karlin and Altschul  Proc. Natl. Acad. Sci. USA  90:5873-77, 1993 (e.g., BLAST®, NBLAST®, XBLAST® or Gapped BLAST® programs, using default parameters of the respective programs). 
     In some embodiments, a sequence, including a nucleic acid or amino acid sequence, is found to have a specified percent identity to a reference sequence, such as a sequence disclosed in this application and/or recited in the claims when sequence identity is determined using the Smith-Waterman algorithm (Smith, T. F. &amp; Waterman, M. S. (1981) “Identification of common molecular subsequences.”  J. Mol. Biol.  147:195-197) or the Needleman-Wunsch algorithm (Needleman, S. B. &amp; Wunsch, C. D. (1970) “A general method applicable to the search for similarities in the amino acid sequences of two proteins.” J. Mol. Biol. 48:443-453) using default parameters. 
     In some embodiments, a sequence, including a nucleic acid or amino acid sequence, is found to have a specified percent identity to a reference sequence, such as a sequence disclosed in this application and/or recited in the claims when sequence identity is determined using a Fast Optimal Global Sequence Alignment Algorithm (FOGSAA) using default parameters. 
     In some embodiments, a sequence, including a nucleic acid or amino acid sequence, is found to have a specified percent identity to a reference sequence, such as a sequence disclosed in this application and/or recited in the claims when sequence identity is determined using Clustal Omega (Sievers et al.,  Mol Syst Biol.  2011 Oct. 11; 7:539) using default parameters. 
     As used in this application, a residue (such as a nucleic acid residue or an amino acid residue) in sequence “X” is referred to as corresponding to a position or residue (such as a nucleic acid residue or an amino acid residue) “Z” in a different sequence “Y” when the residue in sequence “X” is at the counterpart position of “Z” in sequence “Y” when sequences X and Y are aligned using amino acid sequence alignment tools known in the art. 
     As used in this application, variant sequences may be homologous sequences. As used in this application, homologous sequences are sequences (e.g., nucleic acid or amino acid sequences) that share a certain percent identity (e.g., at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, at least 76%, at least 77%, at least 78%, at least 79%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% percent identity, including all values in between). Homologous sequences include but are not limited to paralogous or orthologous sequences. Paralogous sequences arise from duplication of a gene within a genome of a species, while orthologous sequences diverge after a speciation event. 
     In some embodiments, a polypeptide variant (e.g., AAE, PKS, PKC, PT, or TS enzyme variant) comprises a domain that shares a secondary structure (e.g., alpha helix, beta sheet) with a reference polypeptide (e.g., a reference AAE, PKS, PKC, PT, or TS enzyme). In some embodiments, a polypeptide variant (e.g., AAE, PKS, PKC, PT, or TS enzyme variant) shares a tertiary structure with a reference polypeptide (e.g., a reference AAE, PKS, PKC, PT, or TS enzyme). As a non-limiting example, a polypeptide variant (e.g., AAE, PKS, PKC, PT, or TS enzyme) may have low primary sequence identity (e.g., less than 80%, less than 75%, less than 70%, less than 65%, less than 60%, less than 55%, less than 50%, less than 45%, less than 40%, less than 35%, less than 30%, less than 25%, less than 20%, less than 15%, less than 10%, or less than 5% sequence identity) compared to a reference polypeptide, but share one or more secondary structures (e.g., including but not limited to loops, alpha helices, or beta sheets), or have the same tertiary structure as a reference polypeptide. For example, a loop may be located between a beta sheet and an alpha helix, between two alpha helices, or between two beta sheets. Homology modeling may be used to compare two or more tertiary structures. 
     Functional variants of the recombinant AAE, PKS, PKC, PT, or TS enzyme disclosed in this application are encompassed by the present disclosure. For example, functional variants may bind one or more of the same substrates or produce one or more of the same products. Functional variants may be identified using any method known in the art. For example, the algorithm of Karlin and Altschul  Proc. Natl. Acad. Sci. USA  87:2264-68, 1990 described above may be used to identify homologous proteins with known functions. 
     Putative functional variants may also be identified by searching for polypeptides with functionally annotated domains. Databases including Pfam (Sonnhammer et al.,  Proteins.  1997 July; 28(3):405-20) may be used to identify polypeptides with a particular domain. 
     Homology modeling may also be used to identify amino acid residues that are amenable to mutation (e.g., substitution, deletion, and/or insertion) without affecting function. A non-limiting example of such a method may include use of position-specific scoring matrix (PSSM) and an energy minimization protocol. 
     Position-specific scoring matrix (PSSM) uses a position weight matrix to identify consensus sequences (e.g., motifs). PSSM can be conducted on nucleic acid or amino acid sequences. Sequences are aligned and the method takes into account the observed frequency of a particular residue (e.g., an amino acid or a nucleotide) at a particular position and the number of sequences analyzed. See, e.g., Stormo et al.,  Nucleic Acids Res.  1982 May 11; 10(9):2997-3011. The likelihood of observing a particular residue at a given position can be calculated. Without being bound by a particular theory, positions in sequences with high variability may be amenable to mutation (e.g., substitution, deletion, and/or insertion; e.g., PSSM score ≥0) to produce functional homologs. 
     PSSM may be paired with calculation of a Rosetta energy function, which determines the difference between the wild-type and the single-point mutant. The Rosetta energy function calculates this difference as (ΔΔG calc ). With the Rosetta function, the bonding interactions between a mutated residue and the surrounding atoms are used to determine whether a mutation increases or decreases protein stability. For example, a mutation that is designated as favorable by the PSSM score (e.g. PSSM score ≥0), can then be analyzed using the Rosetta energy function to determine the potential impact of the mutation on protein stability. Without being bound by a particular theory, potentially stabilizing amino acid mutations are desirable for protein engineering (e.g., production of functional homologs). In some embodiments, a potentially stabilizing amino acid mutation has a ΔΔG calc  value of less than −0.1 (e.g., less than −0.2, less than −0.3, less than −0.35, less than −0.4, less than −0.45, less than −0.5, less than −0.55, less than −0.6, less than −0.65, less than −0.7, less than −0.75, less than −0.8, less than −0.85, less than −0.9, less than −0.95, or less than −1.0) Rosetta energy units (R.e.u.). See, e.g., Goldenzweig et al.,  Mol Cell.  2016 Jul. 21; 63(2):337-346. Doi: 10.1016/j.molcel.2016.06.012. 
     In some embodiments, a coding sequence comprises an amino acid mutation at 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 or more than 100 positions relative to a reference coding sequence. In some embodiments, the coding sequence comprises an amino acid mutation in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 or more codons of the coding sequence relative to a reference coding sequence. As will be understood by one of ordinary skill in the art, a substitution, insertion, or deletion within a codon may or may not change the amino acid that is encoded by the codon due to degeneracy of the genetic code. In some embodiments, the one or more substitutions, insertions, or deletions in the coding sequence do not alter the amino acid sequence of the coding sequence relative to the amino acid sequence of a reference polypeptide. 
     In some embodiments, the one or more mutations in a sequence do alter the amino acid sequence of the corresponding polypeptide relative to the amino acid sequence of a reference polypeptide. In some embodiments, the one or more mutations alters the amino acid sequence of the polypeptide relative to the amino acid sequence of a reference polypeptide and alter (enhance or reduce) an activity of the polypeptide relative to the reference polypeptide. 
     The activity (e.g., specific activity) of any of the recombinant polypeptides described in this application (e.g., AAE, PKS, PKC, PT, or TS) may be measured using routine methods. As a non-limiting example, a recombinant polypeptide&#39;s activity may be determined by measuring its substrate specificity, product(s) produced, the concentration of product(s) produced, or any combination thereof. As used in this application, “specific activity” of a recombinant polypeptide refers to the amount (e.g., concentration) of a particular product produced for a given amount (e.g., concentration) of the recombinant polypeptide per unit time. 
     The skilled artisan will also realize that mutations in a coding sequence may result in conservative amino acid substitutions to provide functionally equivalent variants of the foregoing polypeptides, e.g., variants that retain the activities of the polypeptides. As used in this application, a “conservative amino acid substitution” refers to an amino acid substitution that does not alter the relative charge or size characteristics or functional activity of the protein in which the amino acid substitution is made. 
     In some instances, an amino acid is characterized by its R group (see, e.g., Table 4). For example, an amino acid may comprise a nonpolar aliphatic R group, a positively charged R group, a negatively charged R group, a nonpolar aromatic R group, or a polar uncharged R group. Non-limiting examples of an amino acid comprising a nonpolar aliphatic R group include alanine, glycine, valine, leucine, methionine, and isoleucine. Non-limiting examples of an amino acid comprising a positively charged R group includes lysine, arginine, and histidine. Non-limiting examples of an amino acid comprising a negatively charged R group include aspartate and glutamate. Non-limiting examples of an amino acid comprising a nonpolar, aromatic R group include phenylalanine, tyrosine, and tryptophan. Non-limiting examples of an amino acid comprising a polar uncharged R group include serine, threonine, cysteine, proline, asparagine, and glutamine. 
     Non-limiting examples of functionally equivalent variants of polypeptides may include conservative amino acid substitutions in the amino acid sequences of proteins disclosed in this application. As used in this application “conservative substitution” is used interchangeably with “conservative amino acid substitution” and refers to any one of the amino acid substitutions provided in Table 4. 
     In some embodiments, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or more than 20 residues can be changed when preparing variant polypeptides. In some embodiments, amino acids are replaced by conservative amino acid substitutions. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Conservative Amino Acid Substitutions 
               
            
           
           
               
               
               
               
            
               
                   
                 Original 
                   
                 Conservative Amino 
               
               
                   
                 Residue 
                 R Group Type 
                 Acid Substitutions 
               
               
                   
                   
               
               
                   
                 Ala 
                 nonpolar aliphatic R group 
                 Cys, Gly, Ser 
               
               
                   
                 Arg 
                 positively charged R group 
                 His, Lys 
               
               
                   
                 Asn 
                 polar uncharged R group 
                 Asp, Gln, Glu 
               
               
                   
                 Asp 
                 negatively charged R group 
                 Asn, Gln, Glu 
               
               
                   
                 Cys 
                 polar uncharged R group 
                 Ala, Ser 
               
               
                   
                 Gln 
                 polar uncharged R group 
                 Asn, Asp, Glu 
               
               
                   
                 Glu 
                 negatively charged R group 
                 Asn, Asp, Gln 
               
               
                   
                 Gly 
                 nonpolar aliphatic R group 
                 Ala, Ser 
               
               
                   
                 His 
                 positively charged R group 
                 Arg, Tyr, Trp 
               
               
                   
                 Ile 
                 nonpolar aliphatic R group 
                 Leu, Met, Val 
               
               
                   
                 Leu 
                 nonpolar aliphatic R group 
                 Ile, Met, Val 
               
               
                   
                 Lys 
                 positively charged R group 
                 Arg, His 
               
               
                   
                 Met 
                 nonpolar aliphatic R group 
                 Ile, Leu, Phe, Val 
               
               
                   
                 Pro 
                 polar uncharged R group 
               
               
                   
                 Phe 
                 nonpolar aromatic R group 
                 Met, Trp, Tyr 
               
               
                   
                 Ser 
                 polar uncharged R group 
                 Ala, Gly, Thr 
               
               
                   
                 Thr 
                 polar uncharged R group 
                 Ala, Asn, Ser 
               
               
                   
                 Trp 
                 nonpolar aromatic R group 
                 His, Phe, Tyr, Met 
               
               
                   
                 Tyr 
                 nonpolar aromatic R group 
                 His, Phe, Trp 
               
               
                   
                 Val 
                 nonpolar aliphatic R group 
                 Ile, Leu, Met, Thr 
               
               
                   
                   
               
            
           
         
       
     
     Amino acid substitutions in the amino acid sequence of a polypeptide to produce a recombinant polypeptide (e.g., AAE, PKS, PKC, PT, or TS) variant having a desired property and/or activity can be made by alteration of the coding sequence of the polypeptide (e.g., AAE, PKS, PKC, PT, or TS). Similarly, conservative amino acid substitutions in the amino acid sequence of a polypeptide to produce functionally equivalent variants of the polypeptide typically are made by alteration of the coding sequence of the recombinant polypeptide (e.g., AAE, PKS, PKC, PT, or TS). 
     Mutations (e.g., substitutions, insertions, additions, or deletions) can be made in a nucleic acid sequence by a variety of methods known to one of ordinary skill in the art. For example, mutations (e.g., substitutions, insertions, additions, or deletions) can be made by PCR-directed mutation, site-directed mutagenesis according to the method of Kunkel (Kunkel,  Proc. Nat. Acad. Sci. U.S.A.  82: 488-492, 1985), by chemical synthesis of a gene encoding a polypeptide, by CRISPR, or by insertions, such as insertion of a tag (e.g., a HIS tag or a GFP tag). Mutations can include, for example, substitutions, insertions, additions, deletions, and translocations, generated by any method known in the art. Methods for producing mutations may be found in in references such as Molecular Cloning: A Laboratory Manual, J. Sambrook, et al., eds., Fourth Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 2012, or Current Protocols in Molecular Biology, F. M. Ausubel, et al., eds., John Wiley &amp; Sons, Inc., New York, 2010. 
     In some embodiments, methods for producing variants include circular permutation (Yu and Lutz,  Trends Biotechnol.  2011 January; 29(1):18-25). In circular permutation, the linear primary sequence of a polypeptide can be circularized (e.g., by joining the N-terminal and C-terminal ends of the sequence) and the polypeptide can be severed (“broken”) at a different location. Thus, the linear primary sequence of the new polypeptide may have low sequence identity (e.g., less than 80%, less than 75%, less than 70%, less than 65%, less than 60%, less than 55%, less than 50%, less than 45%, less than 40%, less than 35%, less than 30%, less than 25%, less than 20%, less than 15%, less than 10%, less or less than 5%, including all values in between) as determined by linear sequence alignment methods (e.g., Clustal Omega or BLAST). Topological analysis of the two proteins, however, may reveal that the tertiary structure of the two polypeptides is similar or dissimilar. Without being bound by a particular theory, a variant polypeptide created through circular permutation of a reference polypeptide and with a similar tertiary structure as the reference polypeptide can share similar functional characteristics (e.g., enzymatic activity, enzyme kinetics, substrate specificity or product specificity). In some instances, circular permutation may alter the secondary structure, tertiary structure or quaternary structure and produce an enzyme with different functional characteristics (e.g., increased or decreased enzymatic activity, different substrate specificity, or different product specificity). See, e.g., Yu and Lutz,  Trends Biotechnol.  2011 January; 29(1):18-25. 
     It should be appreciated that in a protein that has undergone circular permutation, the linear amino acid sequence of the protein would differ from a reference protein that has not undergone circular permutation. However, one of ordinary skill in the art would be able to determine which residues in the protein that has undergone circular permutation correspond to residues in the reference protein that has not undergone circular permutation by, for example, aligning the sequences and detecting conserved motifs, and/or by comparing the structures or predicted structures of the proteins, e.g., by homology modeling. 
     In some embodiments, an algorithm that determines the percent identity between a sequence of interest and a reference sequence described in this application accounts for the presence of circular permutation between the sequences. The presence of circular permutation may be detected using any method known in the art, including, for example, RASPODOM (Weiner et al., Bioinformatics. 2005 Apr. 1; 21(7):932-7). In some embodiments, the presence of circulation permutation is corrected for (e.g., the domains in at least one sequence are rearranged) prior to calculation of the percent identity between a sequence of interest and a sequence described in this application. The claims of this application should be understood to encompass sequences for which percent identity to a reference sequence is calculated after taking into account potential circular permutation of the sequence. 
     Expression of Nucleic Acids in Host Cells 
     Aspects of the present disclosure relate to recombinant enzymes, functional modifications and variants thereof, as well as their uses. For example, the methods described in this application may be used to produce cannabinoids and/or cannabinoid precursors. The methods may comprise using a host cell comprising an enzyme disclosed in this application, cell lysate, isolated enzymes, or any combination thereof. Methods comprising recombinant expression of genes encoding an enzyme disclosed in this application in a host cell are encompassed by the present disclosure. In vitro methods comprising reacting one or more cannabinoid precursors or cannabinoids in a reaction mixture with an enzyme disclosed in this application are also encompassed by the present disclosure. In some embodiments, the enzyme is a TS. 
     A nucleic acid encoding any of the recombinant polypeptides (e.g., AAE, PKS, PKC, PT, or TS enzyme) described in this application may be incorporated into any appropriate vector through any method known in the art. For example, the vector may be an expression vector, including but not limited to a viral vector (e.g., a lentiviral, retroviral, adenoviral, or adeno-associated viral vector), any vector suitable for transient expression, any vector suitable for constitutive expression, or any vector suitable for inducible expression (e.g., a galactose-inducible or doxycycline-inducible vector). 
     A vector encoding any of the recombinant polypeptides (e.g., AAE, PKS, PKC, PT, or TS enzyme) described in this application may be introduced into a suitable host cell using any method known in the art. Non-limiting examples of yeast transformation protocols are described in Gietz et al., Yeast transformation can be conducted by the LiAc/SS Carrier DNA/PEG method.  Methods Mol Biol.  2006; 313:107-20, which is hereby incorporated by reference in its entirety. Host cells may be cultured under any conditions suitable as would be understood by one of ordinary skill in the art. For example, any media, temperature, and incubation conditions known in the art may be used. For host cells carrying an inducible vector, cells may be cultured with an appropriate inducible agent to promote expression. 
     In some embodiments, a vector replicates autonomously in the cell. In some embodiments, a vector integrates into a chromosome within a cell. A vector can contain one or more endonuclease restriction sites that are cut by a restriction endonuclease to insert and ligate a nucleic acid containing a gene described in this application to produce a recombinant vector that is able to replicate in a cell. Vectors are typically composed of DNA, although RNA vectors are also available. Cloning vectors include, but are not limited to: plasmids, fosmids, phagemids, virus genomes and artificial chromosomes. As used in this application, the terms “expression vector” or “expression construct” refer to a nucleic acid construct, generated recombinantly or synthetically, with a series of specified nucleic acid elements that permit transcription of a particular nucleic acid in a host cell (e.g., microbe), such as a yeast cell. In some embodiments, the nucleic acid sequence of a gene described in this application is inserted into a cloning vector so that it is operably joined to regulatory sequences and, in some embodiments, expressed as an RNA transcript. In some embodiments, the vector contains one or more markers, such as a selectable marker as described in this application, to identify cells transformed or transfected with the recombinant vector. In some embodiments, a host cell has already been transformed with one or more vectors. In some embodiments, a host cell that has been transformed with one or more vectors is subsequently transformed with one or more vectors. In some embodiments, a host cell is transformed simultaneously with more than one vector. In some embodiments, a cell that has been transformed with a vector or an expression cassette incorporates all or part of the vector or expression cassette into its genome. In some embodiments, the nucleic acid sequence of a gene described in this application is recoded. Recoding may increase production of the gene product by at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or 100%, including all values in between) relative to a reference sequence that is not recoded. 
     In some embodiments, the nucleic acid encoding any of the proteins described in this application is under the control of regulatory sequences (e.g., enhancer sequences). In some embodiments, a nucleic acid is expressed under the control of a promoter. The promoter can be a native promoter, e.g., the promoter of the gene in its endogenous context, which provides normal regulation of expression of the gene. Alternatively, a promoter can be a promoter that is different from the native promoter of the gene, e.g., the promoter is different from the promoter of the gene in its endogenous context. 
     In some embodiments, the promoter is a eukaryotic promoter. Non-limiting examples of eukaryotic promoters include TDH3, PGK1, PKC1, PDC1, TEF1, TEF2, RPL18B, SSA1, TDH2, PYK1, TPI1, GAL1, GAL10, GAL7, GAL3, GAL2, MET3, MET25, HXT3, HXT7, ACT1, ADH1, ADH2, CUP1-1, ENO2, and SOD1, as would be known to one of ordinary skill in the art (see, e.g., Addgene website: blog.addgene.org/plasmids-101-the-promoter-region). In some embodiments, the promoter is a prokaryotic promoter (e.g., bacteriophage or bacterial promoter). Non-limiting examples of bacteriophage promoters include Pls1con, T3, T7, SP6, and PL. Non-limiting examples of bacterial promoters include Pbad, PmgrB, Ptrc2, Plac/ara, Ptac, and Pm. 
     In some embodiments, the promoter is an inducible promoter. As used in this application, an “inducible promoter” is a promoter controlled by the presence or absence of a molecule. This may be used, for example, to controllably induce the expression of an enzyme. In some embodiments, an inducible promoter linked to an enzyme may be used to regulate expression of the enzyme(s), for example to reduce cannabinoid production in certain scenarios (e.g., during transport of the genetically modified organism to satisfy regulatory restrictions in certain jurisdictions, or between jurisdictions, where cannabinoids may not be shipped). In some embodiments, an inducible promoter linked to an enzyme may be used to regulate expression of the enzyme(s), for example to reduce cannabinoid production in certain scenarios (e.g., during transport of the genetically modified organism to satisfy regulatory restrictions in certain jurisdictions, or between jurisdictions, where cannabinoids may not be shipped). Non-limiting examples of inducible promoters include chemically regulated promoters and physically regulated promoters. For chemically regulated promoters, the transcriptional activity can be regulated by one or more compounds, such as alcohol, tetracycline, galactose, a steroid, a metal, an amino acid, or other compounds. For physically regulated promoters, transcriptional activity can be regulated by a phenomenon such as light or temperature. Non-limiting examples of tetracycline-regulated promoters include anhydrotetracycline (aTc)-responsive promoters and other tetracycline-responsive promoter systems (e.g., a tetracycline repressor protein (tetR), a tetracycline operator sequence (tetO) and a tetracycline transactivator fusion protein (tTA)). Non-limiting examples of steroid-regulated promoters include promoters based on the rat glucocorticoid receptor, human estrogen receptor, moth ecdysone receptors, and promoters from the steroid/retinoid/thyroid receptor superfamily. Non-limiting examples of metal-regulated promoters include promoters derived from metallothionein (proteins that bind and sequester metal ions) genes. Non-limiting examples of pathogenesis-regulated promoters include promoters induced by salicylic acid, ethylene or benzothiadiazole (BTH). Non-limiting examples of temperature/heat-inducible promoters include heat shock promoters. Non-limiting examples of light-regulated promoters include light responsive promoters from plant cells. In certain embodiments, the inducible promoter is a galactose-inducible promoter. In some embodiments, the inducible promoter is induced by one or more physiological conditions (e.g., pH, temperature, radiation, osmotic pressure, saline gradients, cell surface binding, or concentration of one or more extrinsic or intrinsic inducing agents). Non-limiting examples of an extrinsic inducer or inducing agent include amino acids and amino acid analogs, saccharides and polysaccharides, nucleic acids, protein transcriptional activators and repressors, cytokines, toxins, petroleum-based compounds, metal containing compounds, salts, ions, enzyme substrate analogs, hormones or any combination. 
     In some embodiments, the promoter is a constitutive promoter. As used in this application, a “constitutive promoter” refers to an unregulated promoter that allows continuous transcription of a gene. Non-limiting examples of a constitutive promoter include TDH3, PGK1, PKC1, PDC1, TEF1, TEF2, RPL18B, SSA1, TDH2, PYK1, TPI1, HXT3, HXT7, ACT1, ADH1, ADH2, ENO2, and SOD1. 
     Other inducible promoters or constitutive promoters, including synthetic promoters, that may be known to one of ordinary skill in the art are also contemplated. 
     The precise nature of the regulatory sequences needed for gene expression may vary between species or cell types, but generally include, as necessary, 5′ non-transcribed and 5′ non-translated sequences involved with the initiation of transcription and translation respectively, such as a TATA box, capping sequence, CAAT sequence, and the like. In particular, such 5′ non-transcribed regulatory sequences will include a promoter region which includes a promoter sequence for transcriptional control of the operably joined gene. Regulatory sequences may also include enhancer sequences or upstream activator sequences. The vectors disclosed may include 5′ leader or signal sequences. The regulatory sequence may also include a terminator sequence. In some embodiments, a terminator sequence marks the end of a gene in DNA during transcription. The choice and design of one or more appropriate vectors suitable for inducing expression of one or more genes described in this application in a heterologous organism is within the ability and discretion of one of ordinary skill in the art. 
     Expression vectors containing the necessary elements for expression are commercially available and known to one of ordinary skill in the art (see, e.g., Sambrook et al., Molecular Cloning: A Laboratory Manual, Fourth Edition, Cold Spring Harbor Laboratory Press, 2012). 
     Host Cells 
     The disclosed cannabinoid biosynthetic methods and host cells are exemplified with  S. cerevisiae , but are also applicable to other host cells, as would be understood by one of ordinary skill in the art. 
     Suitable host cells include, but are not limited to: yeast cells, bacterial cells, algal cells, plant cells, fungal cells, insect cells, and animal cells, including mammalian cells. In one illustrative embodiment, suitable host cells include  E. coli  (e.g., Shuffle™ competent  E. coli  available from New England BioLabs in Ipswich, Mass.). 
     Other suitable host cells of the present disclosure include microorganisms of the genus  Corynebacterium . In some embodiments, preferred  Corynebacterium  strains/species include:  C. efficiens , with the deposited type strain being DSM44549,  C. glutamicum , with the deposited type strain being ATCC13032, and  C. ammoniagenes , with the deposited type strain being ATCC6871. In some embodiments the preferred host cell of the present disclosure is  C. glutamicum.    
     Suitable host cells of the genus  Corynebacterium , in particular of the species  Corynebacterium glutamicum , are in particular the known wild-type strains:  Corynebacterium glutamicum  ATCC13032,  Corynebacterium acetoglutamicum  ATCC15806,  Corynebacterium acetoacidophilum  ATCC13870,  Corynebacterium melassecola  ATCC17965,  Corynebacterium thermoaminogenes  FERM BP-1539,  Brevibacterium flavum  ATCC14067,  Brevibacterium lactofermentum  ATCC13869, and  Brevibacterium divaricatum  ATCC14020; and L-amino acid-producing mutants, or strains, prepared therefrom, such as, for example, the L-lysine-producing strains:  Corynebacterium glutamicum  FERM-P 1709,  Brevibacterium flavum  FERM-P 1708,  Brevibacterium lactofermentum  FERM-P 1712,  Corynebacterium glutamicum  FERM-P 6463,  Corynebacterium glutamicum  FERM-P 6464,  Corynebacterium glutamicum  DM58-1,  Corynebacterium glutamicum  DG52-5,  Corynebacterium glutamicum  DSM5714, and  Corynebacterium glutamicum  DSM12866. 
     Suitable yeast host cells include, but are not limited to:  Candida, Hansenula, Saccharomyces, Schizosaccharomyces, Pichia, Kluyveromyces , and  Yarrowia . In some embodiments, the yeast cell is  Hansenula polymorpha, Saccharomyces cerevisiae, Saccaromyces carlsbergensis, Saccharomyces diastaticus, Saccharomyces norbensis, Saccharomyces kluyveri, Schizosaccharomyces pombe, Komagataella phaffii , formerly known as  Pichia pastoris, Pichia finlandica, Pichia trehalophila, Pichia kodamae, Pichia membranaefaciens, Pichia opuntiae, Pichia thermotolerans, Pichia salictaria, Pichia quercuum, Pichia pijperi, Pichia stipitis, Pichia methanolica, Pichia angusta, Kluyveromyces lactis, Candida albicans , or  Yarrowia lipolytica.    
     In some embodiments, the yeast strain is an industrial polyploid yeast strain. Other non-limiting examples of fungal cells include cells obtained from  Aspergillus  spp.,  Penicillium  spp.,  Fusarium  spp.,  Rhizopus  spp.,  Acremonium  spp.,  Neurospora  spp.,  Sordaria  spp.,  Magnaporthe  spp.,  Allomyces  spp.,  Ustilago  spp.,  Botrytis  spp., and  Trichoderma  spp. 
     In certain embodiments, the host cell is an algal cell such as,  Chlamydomonas  (e.g.,  C. reinhardtii ) and  Phormidium  (P. sp. ATCC29409). 
     In other embodiments, the host cell is a prokaryotic cell. Suitable prokaryotic cells include gram positive, gram negative, and gram-variable bacterial cells. The host cell may be a species of, but not limited to:  Agrobacterium, Alicyclobacillus, Anabaena, Anacystis, Acinetobacter, Acidothermus, Arthrobacter, Azobacter, Bacillus, Bifidobacterium, Brevibacterium, Butyrivibrio, Buchnera, Campestris, Camplyobacter, Clostridium, Corynebacterium, Chromatium, Coprococcus, Escherichia, Enterococcus, Enterobacter, Erwinia, Fusobacterium, Faecalibacterium, Francisella, Flavobacterium, Geobacillus, Haemophilus, Helicobacter, Klebsiella, Lactobacillus, Lactococcus, Ilyobacter, Micrococcus, Microbacterium, Mesorhizobium, Methylobacterium, Methylobacterium, Mycobacterium, Neisseria, Pantoea, Pseudomonas, Prochlorococcus, Rhodobacter, Rhodopseudomonas, Rhodopseudomonas, Roseburia, Rhodospirillum, Rhodococcus, Scenedesmus, Streptomyces, Streptococcus, Synecoccus, Saccharomonospora, Saccharopolyspora, Staphylococcus, Serratia, Salmonella, Shigella, Thermoanaerobacterium, Tropheryma, Tularensis, Temecula, Thermosynechococcus, Thermococcus, Ureaplasma, Xanthomonas, Xylella, Yersinia , and  Zymomonas.    
     In some embodiments, the bacterial host strain is an industrial strain. Numerous bacterial industrial strains are known and suitable for the methods and compositions described in this application. 
     In some embodiments, the bacterial host cell is of the  Agrobacterium  species (e.g.,  A. radiobacter, A. rhizogenes, A. rubi ), the  Arthrobacter  species (e.g.,  A. aurescens, A. citreus, A. globformis, A. hydrocarboglutamicus, A. mysorens, A. nicotianae, A. paraffineus, A. protophonniae, A. roseoparaffinus, A. sulfureus, A. ureafaciens ), the  Bacillus  species (e.g.,  B. thuringiensis, B. anthracis, B. megaterium, B. subtilis, B. lentus, B. circulars, B. pumilus, B. lautus, B. coagulans, B. brevis, B. firmus, B. alkaophius, B. licheniformis, B. clausii, B. stearothermophilus, B. halodurans  and  B. amyloliquefaciens . In particular embodiments, the host cell will be an industrial  Bacillus  strain including but not limited to  B. subtilis, B. pumilus, B. licheniformis, B. megaterium, B. clausii, B. stearothermophilus  and  B. amyloliquefaciens . In some embodiments, the host cell will be an industrial  Clostridium  species (e.g.,  C. acetobutylicum, C. tetani  E88,  C. lituseburense, C. saccharobutylicum, C. perfringens, C. beijerinckii ). In some embodiments, the host cell will be an industrial  Corynebacterium  species (e.g.,  C. glutamicum, C. acetoacidophilum ). In some embodiments, the host cell will be an industrial  Escherichia  species (e.g.,  E. coli ). In some embodiments, the host cell will be an industrial  Erwinia  species (e.g.,  E. uredovora, E. carotovora, E. ananas, E. herbicola, E. punctata, E. terreus ). In some embodiments, the host cell will be an industrial  Pantoea  species (e.g.,  P. citrea, P. agglomerans ). In some embodiments, the host cell will be an industrial  Pseudomonas  species, (e.g.,  P. putida, P. aeruginosa, P. mevalonii ). In some embodiments, the host cell will be an industrial  Streptococcus  species (e.g.,  S. equisimiles, S. pyogenes, S. uberis ). In some embodiments, the host cell will be an industrial  Streptomyces  species (e.g.,  S. ambofaciens, S. achromogenes, S. avermitilis, S. coelicolor, S. aureofaciens, S. aureus, S. fungicidicus, S. griseus, S. lividans ). In some embodiments, the host cell will be an industrial  Zymomonas  species (e.g.,  Z. mobilis, Z. lipolytica ), and the like. 
     The present disclosure is also suitable for use with a variety of animal cell types, including mammalian cells, for example, human (including 293, HeLa, W138, PER.C6 and Bowes melanoma cells), mouse (including 3T3, NS0, NS1, Sp2/0), hamster (CHO, BHK), monkey (COS, FRhL, Vero), insect cells, for example fall armyworm (including Sf9 and Sf21), silkmoth (including BmN), cabbage looper (including BTI-Tn-5B1-4) and common fruit fly (including Schneider 2), and hybridoma cell lines. 
     In various embodiments, strains that may be used in the practice of the disclosure including both prokaryotic and eukaryotic strains, and are readily accessible to the public from a number of culture collections such as American Type Culture Collection (ATCC), Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSM), Centraalbureau Voor Schimmelcultures (CBS), and Agricultural Research Service Patent Culture Collection, Northern Regional Research Center (NRRL). The present disclosure is also suitable for use with a variety of plant cell types. In some embodiments, the plant is of the  Cannabis  genus in the family Cannabaceae. In certain embodiments, the plant is of the species  Cannabis sativa, Cannabis indica , or  Cannabis ruderalis . In other embodiments, the plant is of the genus  Nicotiana  in the family Solanaceae. In certain embodiments, the plant is of the species  Nicotiana rustica.    
     The term “cell,” as used in this application, may refer to a single cell or a population of cells, such as a population of cells belonging to the same cell line or strain. Use of the singular term “cell” should not be construed to refer explicitly to a single cell rather than a population of cells. The host cell may comprise genetic modifications relative to a wild-type counterpart. Reduction of gene expression and/or gene inactivation in a host cell may be achieved through any suitable method, including but not limited to, deletion of the gene, introduction of a point mutation into the gene, selective editing of the gene and/or truncation of the gene. For example, polymerase chain reaction (PCR)-based methods may be used (see, e.g., Gardner et al.,  Methods Mol Biol.  2014; 1205:45-78). As a non-limiting example, genes may be deleted through gene replacement (e.g., with a marker, including a selection marker). A gene may also be truncated through the use of a transposon system (see, e.g., Poussu et al.,  Nucleic Acids Res.  2005; 33(12): e104). A gene may also be edited through of the use of gene editing technologies known in the art, such as CRISPR-based technologies. 
     Culturing of Host Cells 
     Any of the cells disclosed in this application can be cultured in media of any type (rich or minimal) and any composition prior to, during, and/or after contact and/or integration of a nucleic acid. The conditions of the culture or culturing process can be optimized through routine experimentation as would be understood by one of ordinary skill in the art. In some embodiments, the selected media is supplemented with various components. In some embodiments, the concentration and amount of a supplemental component is optimized. In some embodiments, other aspects of the media and growth conditions (e.g., pH, temperature, etc.) are optimized through routine experimentation. In some embodiments, the frequency that the media is supplemented with one or more supplemental components, and the amount of time that the cell is cultured, is optimized. 
     Culturing of the cells described in this application can be performed in culture vessels known and used in the art. In some embodiments, an aerated reaction vessel (e.g., a stirred tank reactor) is used to culture the cells. In some embodiments, a bioreactor or fermenter is used to culture the cell. Thus, in some embodiments, the cells are used in fermentation. As used in this application, the terms “bioreactor” and “fermenter” are interchangeably used and refer to an enclosure, or partial enclosure, in which a biological, biochemical and/or chemical reaction takes place that involves a living organism or part of a living organism. A “large-scale bioreactor” or “industrial-scale bioreactor” is a bioreactor that is used to generate a product on a commercial or quasi-commercial scale. Large scale bioreactors typically have volumes in the range of liters, hundreds of liters, thousands of liters, or more. 
     Non-limiting examples of bioreactors include: stirred tank fermenters, bioreactors agitated by rotating mixing devices, chemostats, bioreactors agitated by shaking devices, airlift fermenters, packed-bed reactors, fixed-bed reactors, fluidized bed bioreactors, bioreactors employing wave induced agitation, centrifugal bioreactors, roller bottles, and hollow fiber bioreactors, roller apparatuses (for example benchtop, cart-mounted, and/or automated varieties), vertically-stacked plates, spinner flasks, stirring or rocking flasks, shaken multi-well plates, MD bottles, T-flasks, Roux bottles, multiple-surface tissue culture propagators, modified fermenters, and coated beads (e.g., beads coated with serum proteins, nitrocellulose, or carboxymethyl cellulose to prevent cell attachment). 
     In some embodiments, the bioreactor includes a cell culture system where the cell (e.g., yeast cell) is in contact with moving liquids and/or gas bubbles. In some embodiments, the cell or cell culture is grown in suspension. In other embodiments, the cell or cell culture is attached to a solid phase carrier. Non-limiting examples of a carrier system includes microcarriers (e.g., polymer spheres, microbeads, and microdisks that can be porous or non-porous), cross-linked beads (e.g., dextran) charged with specific chemical groups (e.g., tertiary amine groups), 2D microcarriers including cells trapped in nonporous polymer fibers, 3D carriers (e.g., carrier fibers, hollow fibers, multicartridge reactors, and semi-permeable membranes that can comprising porous fibers), microcarriers having reduced ion exchange capacity, encapsulation cells, capillaries, and aggregates. In some embodiments, carriers are fabricated from materials such as dextran, gelatin, glass, or cellulose. 
     In some embodiments, industrial-scale processes are operated in continuous, semi-continuous or non-continuous modes. Non-limiting examples of operation modes are batch, fed batch, extended batch, repetitive batch, draw/fill, rotating-wall, spinning flask, and/or perfusion mode of operation. In some embodiments, a bioreactor allows continuous or semi-continuous replenishment of the substrate stock, for example a carbohydrate source and/or continuous or semi-continuous separation of the product, from the bioreactor. 
     In some embodiments, the bioreactor or fermenter includes a sensor and/or a control system to measure and/or adjust reaction parameters. Non-limiting examples of reaction parameters include biological parameters (e.g., growth rate, cell size, cell number, cell density, cell type, or cell state, etc.), chemical parameters (e.g., pH, redox-potential, concentration of reaction substrate and/or product, concentration of dissolved gases, such as oxygen concentration and CO 2  concentration, nutrient concentrations, metabolite concentrations, concentration of an oligopeptide, concentration of an amino acid, concentration of a vitamin, concentration of a hormone, concentration of an additive, serum concentration, ionic strength, concentration of an ion, relative humidity, molarity, osmolarity, concentration of other chemicals, for example buffering agents, adjuvants, or reaction by-products), physical/mechanical parameters (e.g., density, conductivity, degree of agitation, pressure, and flow rate, shear stress, shear rate, viscosity, color, turbidity, light absorption, mixing rate, conversion rate, as well as thermodynamic parameters, such as temperature, light intensity/quality, etc.). Sensors to measure the parameters described in this application are well known to one of ordinary skill in the relevant mechanical and electronic arts. Control systems to adjust the parameters in a bioreactor based on the inputs from a sensor described in this application are well known to one of ordinary skill in the art in bioreactor engineering. 
     In some embodiments, the method involves batch fermentation (e.g., shake flask fermentation). General considerations for batch fermentation (e.g., shake flask fermentation) include the level of oxygen and glucose. For example, batch fermentation (e.g., shake flask fermentation) may be oxygen and glucose limited, so in some embodiments, the capability of a strain to perform in a well-designed fed-batch fermentation is underestimated. Also, the final product (e.g., cannabinoid or cannabinoid precursor) may display some differences from the substrate in terms of solubility, toxicity, cellular accumulation and secretion and in some embodiments can have different fermentation kinetics. 
     In some embodiments, the cells of the present disclosure are adapted to produce cannabinoids or cannabinoid precursors in vivo. In some embodiments, the cells are adapted to secrete one or more enzymes for cannabinoid synthesis (e.g., AAE, PKS, PKC, PT, or TS). In some embodiments, the cells of the present disclosure are lysed, and the remaining lysates are recovered for subsequent use. In such embodiments, the secreted or lysed enzyme can catalyze reactions for the production of a cannabinoid or precursor by bioconversion in an in vitro or ex vivo process. In some embodiments, any and all conversions described in this application can be conducted chemically or enzymatically, in vitro or in vivo. 
     In some embodiments, the host cells of the present disclosure are adapted to produce cannabinoids or cannabinoid precursors in vivo. In some embodiments, the host cells are adapted to secrete one or more cannabinoid pathway substrates, intermediates, and/or terminal products (e.g., olivetol, THCA, THC, CBDA, CBD, CBGA, CBGVA, THCVA, CBDVA, CBCVA, or CBCA). In some embodiments, the host cells of the present disclosure are lysed, and the lysate is recovered for subsequent use. In such embodiments, the secreted substrates, intermediates, and/or terminal products may be recovered from the culture media. 
     Purification and Further Processing 
     In some embodiments, any of the methods described in this application may include isolation and/or purification of the cannabinoids and/or cannabinoid precursors produced (e.g., produced in a bioreactor). For example, the isolation and/or purification can involve one or more of cell lysis, centrifugation, extraction, column chromatography, distillation, crystallization, and lyophilization. 
     The methods described in this application encompass production of any cannabinoid or cannabinoid precursor known in the art. Cannabinoids or cannabinoid precursors produced by any of the recombinant cells disclosed in this application or any of the in vitro methods described in this application may be identified and extracted using any method known in the art. Mass spectrometry (e.g., LC-MS, GC-MS) is a non-limiting example of a method for identification and may be used to extract a compound of interest. 
     In some embodiments, any of the methods described in this application further comprise decarboxylation of a cannabinoid or cannabinoid precursor. As a non-limiting example, the acid form of a cannabinoid or cannabinoid precursor may be heated (e.g., at least 90° C.) to decarboxylate the cannabinoid or cannabinoid precursor. See, e.g., U.S. Pat. Nos. 10,159,908, 10,143,706, 9,908,832 and 7,344,736. See also, e.g., Wang et al., Cannabis Cannabinoid Res. 2016; 1(1): 262-271. 
     Compositions, Kits, and Administration 
     The present disclosure provides compositions, including pharmaceutical compositions, comprising a cannabinoid or a cannabinoid precursor, or pharmaceutically acceptable salt thereof, produced by any of the methods described in this application, and optionally a pharmaceutically acceptable excipient. 
     In certain embodiments, a cannabinoid or cannabinoid precursor described in this application is provided in an effective amount in a composition, such as a pharmaceutical composition. In certain embodiments, the effective amount is a therapeutically effective amount. In certain embodiments, the effective amount is a prophylactically effective amount. 
     Compositions, such as pharmaceutical compositions, described in this application can be prepared by any method known in the art. In general, such preparatory methods include bringing a compound described in this application (i.e., the “active ingredient”) into association with a carrier or excipient, and/or one or more other accessory ingredients, and then, if necessary and/or desirable, shaping, and/or packaging the product into a desired single- or multi-dose unit. 
     Pharmaceutical compositions can be prepared, packaged, and/or sold in bulk, as a single unit dose, and/or as a plurality of single unit doses. A “unit dose” is a discrete amount of the pharmaceutical composition comprising a predetermined amount of the active ingredient. The amount of the active ingredient is generally equal to the dosage of the active ingredient which would be administered to a subject and/or a convenient fraction of such a dosage, such as one-half or one-third of such a dosage. 
     Relative amounts of the active ingredient, the pharmaceutically acceptable excipient, and/or any additional ingredients in a pharmaceutical composition described in this application will vary, depending upon the identity, size, and/or condition of the subject treated and further depending upon the route by which the composition is to be administered. The composition may comprise between 0.1% and 100% (w/w) active ingredient. 
     Pharmaceutically acceptable excipients used in the manufacture of pharmaceutical compositions include inert diluents, dispersing and/or granulating agents, surface active agents and/or emulsifiers, disintegrating agents, binding agents, preservatives, buffering agents, lubricating agents, and/or oils. Excipients such as cocoa butter and suppository waxes, coloring agents, coating agents, sweetening, flavoring, and perfuming agents may also be present in the composition. Exemplary excipients include diluents, dispersing and/or granulating agents, surface active agents and/or emulsifiers, disintegrating agents, binding agents, preservatives, buffering agents, lubricating agents, and/or oils (e.g., synthetic oils, semi-synthetic oils) as disclosed in this application. 
     Exemplary diluents include calcium carbonate, sodium carbonate, calcium phosphate, dicalcium phosphate, calcium sulfate, calcium hydrogen phosphate, sodium phosphate lactose, sucrose, cellulose, microcrystalline cellulose, kaolin, mannitol, sorbitol, inositol, sodium chloride, dry starch, cornstarch, powdered sugar, and mixtures thereof. 
     Exemplary granulating and/or dispersing agents include potato starch, corn starch, tapioca starch, sodium starch glycolate, clays, alginic acid, guar gum, citrus pulp, agar, bentonite, cellulose, and wood products, natural sponge, cation-exchange resins, calcium carbonate, silicates, sodium carbonate, cross-linked poly(vinyl-pyrrolidone) (crospovidone), sodium carboxymethyl starch (sodium starch glycolate), carboxymethyl cellulose, cross-linked sodium carboxymethyl cellulose (croscarmellose), methylcellulose, pregelatinized starch (starch 1500), microcrystalline starch, water insoluble starch, calcium carboxymethyl cellulose, magnesium aluminum silicate (Veegum), sodium lauryl sulfate, quaternary ammonium compounds, and mixtures thereof. 
     Exemplary surface active agents and/or emulsifiers include natural emulsifiers (e.g., acacia, agar, alginic acid, sodium alginate, tragacanth, chondrux, cholesterol, xanthan, pectin, gelatin, egg yolk, casein, wool fat, cholesterol, wax, and lecithin), colloidal clays (e.g., bentonite (aluminum silicate) and Veegum (magnesium aluminum silicate)), long chain amino acid derivatives, high molecular weight alcohols (e.g., stearyl alcohol, cetyl alcohol, oleyl alcohol, triacetin monostearate, ethylene glycol distearate, glyceryl monostearate, and propylene glycol monostearate, polyvinyl alcohol), carbomers (e.g., carboxy polymethylene, polyacrylic acid, acrylic acid polymer, and carboxyvinyl polymer), carrageenan, cellulosic derivatives (e.g., carboxymethylcellulose sodium, powdered cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, methylcellulose), sorbitan fatty acid esters (e.g., polyoxyethylene sorbitan monolaurate (Tween® 20), polyoxyethylene sorbitan (Tween® 60), polyoxyethylene sorbitan monooleate (Tween® 80), sorbitan monopalmitate (Span® 40), sorbitan monostearate (Span® 60), sorbitan tristearate (Span® 65), glyceryl monooleate, sorbitan monooleate (Span® 80), polyoxyethylene esters (e.g., polyoxyethylene monostearate (Myrj® 45), polyoxyethylene hydrogenated castor oil, polyethoxylated castor oil, polyoxymethylene stearate, and Solutol®), sucrose fatty acid esters, polyethylene glycol fatty acid esters (e.g., Cremophor®), polyoxyethylene ethers, (e.g., polyoxyethylene lauryl ether (Brij® 30)), poly(vinyl-pyrrolidone), diethylene glycol monolaurate, triethanolamine oleate, sodium oleate, potassium oleate, ethyl oleate, oleic acid, ethyl laurate, sodium lauryl sulfate, Pluronic® F-68, poloxamer P-188, cetrimonium bromide, cetylpyridinium chloride, benzalkonium chloride, docusate sodium, and/or mixtures thereof. 
     Exemplary binding agents include starch (e.g., cornstarch and starch paste), gelatin, sugars (e.g., sucrose, glucose, dextrose, dextrin, molasses, lactose, lactitol, mannitol, etc.), natural and synthetic gums (e.g., acacia, sodium alginate, extract of Irish moss, panwar gum, ghatti gum, mucilage of isapol husks, carboxymethylcellulose, methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, microcrystalline cellulose, cellulose acetate, poly(vinyl-pyrrolidone), magnesium aluminum silicate (Veegum©), and larch arabogalactan), alginates, polyethylene oxide, polyethylene glycol, inorganic calcium salts, silicic acid, polymethacrylates, waxes, water, alcohol, and/or mixtures thereof. 
     Exemplary preservatives include antioxidants, chelating agents, antimicrobial preservatives, antifungal preservatives, antiprotozoan preservatives, alcohol preservatives, acidic preservatives, and other preservatives. In certain embodiments, the preservative is an antioxidant. In other embodiments, the preservative is a chelating agent. 
     Exemplary antioxidants include alpha tocopherol, ascorbic acid, acorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, monothioglycerol, potassium metabisulfite, propionic acid, propyl gallate, sodium ascorbate, sodium bisulfite, sodium metabisulfite, and sodium sulfite. 
     Exemplary chelating agents include ethylenediaminetetraacetic acid (EDTA) and salts and hydrates thereof (e.g., sodium edetate, disodium edetate, trisodium edetate, calcium disodium edetate, dipotassium edetate, and the like), citric acid and salts and hydrates thereof (e.g., citric acid monohydrate), fumaric acid and salts and hydrates thereof, malic acid and salts and hydrates thereof, phosphoric acid and salts and hydrates thereof, and tartaric acid and salts and hydrates thereof. Exemplary antimicrobial preservatives include benzalkonium chloride, benzethonium chloride, benzyl alcohol, bronopol, cetrimide, cetylpyridinium chloride, chlorhexidine, chlorobutanol, chlorocresol, chloroxylenol, cresol, ethyl alcohol, glycerin, hexetidine, imidurea, phenol, phenoxyethanol, phenylethyl alcohol, phenylmercuric nitrate, propylene glycol, and thimerosal. 
     Exemplary antifungal preservatives include butyl paraben, methyl paraben, ethyl paraben, propyl paraben, benzoic acid, hydroxybenzoic acid, potassium benzoate, potassium sorbate, sodium benzoate, sodium propionate, and sorbic acid. 
     Exemplary alcohol preservatives include ethanol, polyethylene glycol, phenol, phenolic compounds, bisphenol, chlorobutanol, hydroxybenzoate, and phenylethyl alcohol. 
     Exemplary acidic preservatives include vitamin A, vitamin C, vitamin E, beta-carotene, citric acid, acetic acid, dehydroacetic acid, ascorbic acid, sorbic acid, and phytic acid. 
     Other preservatives include tocopherol, tocopherol acetate, deteroxime mesylate, cetrimide, butylated hydroxyanisol (BHA), butylated hydroxytoluened (BHT), ethylenediamine, sodium lauryl sulfate (SLS), sodium lauryl ether sulfate (SLES), sodium bisulfite, sodium metabisulfite, potassium sulfite, potassium metabisulfite, Glydant® Plus, Phenonip®, methylparaben, Germall® 115, Germaben® II, Neolone®, Kathon®, and Euxyl®. 
     Exemplary buffering agents include citrate buffer solutions, acetate buffer solutions, phosphate buffer solutions, ammonium chloride, calcium carbonate, calcium chloride, calcium citrate, calcium glubionate, calcium gluceptate, calcium gluconate, D-gluconic acid, calcium glycerophosphate, calcium lactate, propanoic acid, calcium levulinate, pentanoic acid, dibasic calcium phosphate, phosphoric acid, tribasic calcium phosphate, calcium hydroxide phosphate, potassium acetate, potassium chloride, potassium gluconate, potassium mixtures, dibasic potassium phosphate, monobasic potassium phosphate, potassium phosphate mixtures, sodium acetate, sodium bicarbonate, sodium chloride, sodium citrate, sodium lactate, dibasic sodium phosphate, monobasic sodium phosphate, sodium phosphate mixtures, tromethamine, magnesium hydroxide, aluminum hydroxide, alginic acid, pyrogen-free water, isotonic saline, Ringer&#39;s solution, ethyl alcohol, and mixtures thereof. 
     Exemplary lubricating agents include magnesium stearate, calcium stearate, stearic acid, silica, talc, malt, glyceryl behanate, hydrogenated vegetable oils, polyethylene glycol, sodium benzoate, sodium acetate, sodium chloride, leucine, magnesium lauryl sulfate, sodium lauryl sulfate, and mixtures thereof. 
     Exemplary natural oils include almond, apricot kernel, avocado, babassu, bergamot, black current seed, borage, cade, camomile, canola, caraway, carnauba, castor, cinnamon, cocoa butter, coconut, cod liver, coffee, corn, cotton seed, emu, eucalyptus, evening primrose, fish, flaxseed, geraniol, gourd, grape seed, hazel nut, hyssop, isopropyl myristate, jojoba, kukui nut, lavandin, lavender, lemon,  Litsea cubeba , macademia nut, mallow, mango seed, meadowfoam seed, mink, nutmeg, olive, orange, orange roughy, palm, palm kernel, peach kernel, peanut, poppy seed, pumpkin seed, rapeseed, rice bran, rosemary, safflower, sandalwood, sasquana, savoury, sea buckthorn, sesame, shea butter, silicone, soybean, sunflower, tea tree, thistle, tsubaki, vetiver, walnut, and wheat germ oils. Exemplary synthetic or semi-synthetic oils include, but are not limited to, butyl stearate, medium chain triglycerides (such as caprylic triglyceride and capric triglyceride), cyclomethicone, diethyl sebacate, dimethicone 360, isopropyl myristate, mineral oil, octyldodecanol, oleyl alcohol, silicone oil, and mixtures thereof. In certain embodiments, exemplary synthetic oils comprise medium chain triglycerides (such as caprylic triglyceride and capric triglyceride). 
     Liquid dosage forms for oral and parenteral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active ingredients, the liquid dosage forms may comprise inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (e.g., cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents. In certain embodiments for parenteral administration, the conjugates described in this application are mixed with solubilizing agents such as Cremophor®, alcohols, oils, modified oils, glycols, polysorbates, cyclodextrins, polymers, and mixtures thereof. 
     Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions can be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation can be a sterile injectable solution, suspension, or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that can be employed are water, Ringer&#39;s solution, U.S.P., and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil can be employed including synthetic mono- or di-glycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables. 
     The injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use. 
     In order to prolong the effect of a drug, it is often desirable to slow the absorption of the drug from subcutaneous or intramuscular injection. This can be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the drug then depends upon its rate of dissolution, which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered drug form may be accomplished by dissolving or suspending the drug in an oil vehicle. 
     Compositions for rectal or vaginal administration are typically suppositories which can be prepared by mixing the conjugates described in this application with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol, or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active ingredient. 
     Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active ingredient is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or (a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, (b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, (c) humectants such as glycerol, (d) disintegrating agents such as agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, (e) solution retarding agents such as paraffin, (f) absorption accelerators such as quaternary ammonium compounds, (g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, (h) absorbents such as kaolin and bentonite clay, and (i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets, and pills, the dosage form may include a buffering agent. 
     Solid compositions of a similar type can be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the art of pharmacology. They may optionally comprise opacifying agents and can be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of encapsulating compositions which can be used include polymeric substances and waxes. Solid compositions of a similar type can be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like. 
     The active ingredient can be in a micro-encapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings, and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the active ingredient can be admixed with at least one inert diluent such as sucrose, lactose, or starch. Such dosage forms may comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose. In the case of capsules, tablets and pills, the dosage forms may comprise buffering agents. They may optionally comprise opacifying agents and can be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of encapsulating agents which can be used include polymeric substances and waxes. 
     Dosage forms for topical and/or transdermal administration of a compound described in this application may include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants, and/or patches. Generally, the active ingredient is admixed under sterile conditions with a pharmaceutically acceptable carrier or excipient and/or any needed preservatives and/or buffers as can be required. Additionally, the present disclosure contemplates the use of transdermal patches, which often have the added advantage of providing controlled delivery of an active ingredient to the body. Such dosage forms can be prepared, for example, by dissolving and/or dispensing the active ingredient in the proper medium. Alternatively or additionally, the rate can be controlled by either providing a rate controlling membrane and/or by dispersing the active ingredient in a polymer matrix and/or gel. 
     Suitable devices for use in delivering intradermal pharmaceutical compositions described in this application include short needle devices. Intradermal compositions can be administered by devices which limit the effective penetration length of a needle into the skin. Alternatively or additionally, conventional syringes can be used in the classical mantoux method of intradermal administration. Jet injection devices which deliver liquid formulations to the dermis via a liquid jet injector and/or via a needle which pierces the stratum corneum and produces a jet which reaches the dermis are suitable. Ballistic powder/particle delivery devices which use compressed gas to accelerate the compound in powder form through the outer layers of the skin to the dermis are suitable. 
     Formulations suitable for topical administration include, but are not limited to, liquid and/or semi-liquid preparations such as liniments, lotions, oil-in-water and/or water-in-oil emulsions such as creams, ointments, and/or pastes, and/or solutions and/or suspensions. Topically administrable formulations may, for example, comprise from about 1% to about 10% (w/w) active ingredient, although the concentration of the active ingredient can be as high as the solubility limit of the active ingredient in the solvent. Formulations for topical administration may further comprise one or more of the additional ingredients described in this application. 
     A pharmaceutical composition described in this application can be prepared, packaged, and/or sold in a formulation suitable for pulmonary administration via the buccal cavity. Such a formulation may comprise dry particles which comprise the active ingredient and which have a diameter in the range from about 0.5 to about 7 nanometers, or from about 1 to about 6 nanometers. Such compositions are conveniently in the form of dry powders for administration using a device comprising a dry powder reservoir to which a stream of propellant can be directed to disperse the powder and/or using a self-propelling solvent/powder dispensing container such as a device comprising the active ingredient dissolved and/or suspended in a low-boiling propellant in a sealed container. Such powders comprise particles wherein at least 98% of the particles by weight have a diameter greater than 0.5 nanometers and at least 95% of the particles by number have a diameter less than 7 nanometers. Alternatively, at least 95% of the particles by weight have a diameter greater than 1 nanometer and at least 90% of the particles by number have a diameter less than 6 nanometers. Dry powder compositions may include a solid fine powder diluent such as sugar and are conveniently provided in a unit dose form. 
     Low boiling propellants generally include liquid propellants having a boiling point of below 65° F. at atmospheric pressure. Generally, the propellant may constitute 50 to 99.9% (w/w) of the composition, and the active ingredient may constitute 0.1 to 20% (w/w) of the composition. The propellant may further comprise additional ingredients such as a liquid non-ionic and/or solid anionic surfactant and/or a solid diluent (which may have a particle size of the same order as particles comprising the active ingredient). 
     Although the descriptions of pharmaceutical compositions provided in this application are principally directed to pharmaceutical compositions which are suitable for administration to humans, it will be understood by the skilled artisan that such compositions are generally suitable for administration to animals of all sorts. Modification of pharmaceutical compositions suitable for administration to humans in order to render the compositions suitable for administration to various animals is well understood, and the ordinarily skilled veterinary pharmacologist can design and/or perform such modification with ordinary experimentation. 
     Compounds provided in this application are typically formulated in dosage unit form for ease of administration and uniformity of dosage. It will be understood, however, that the total daily usage of the compositions described in this application will be decided by a physician within the scope of sound medical judgment. The specific therapeutically effective dose level for any particular subject or organism will depend upon a variety of factors including the disease being treated and the severity of the disorder; the activity of the specific active ingredient employed; the specific composition employed; the age, body weight, general health, sex, and diet of the subject; the time of administration, route of administration, and rate of excretion of the specific active ingredient employed; the duration of the treatment; drugs used in combination or coincidental with the specific active ingredient employed; and like factors well known in the medical arts. 
     The compounds and compositions provided in this application can be administered by any route, including enteral (e.g., oral), parenteral, intravenous, intramuscular, intra-arterial, intramedullary, intrathecal, subcutaneous, intraventricular, transdermal, interdermal, rectal, intravaginal, intraperitoneal, topical (as by powders, ointments, creams, and/or drops), mucosal, nasal, bucal, sublingual; by intratracheal instillation, bronchial instillation, and/or inhalation; and/or as an oral spray, nasal spray, and/or aerosol. Specifically contemplated routes are oral administration, intravenous administration (e.g., systemic intravenous injection), regional administration via blood and/or lymph supply, and/or direct administration to an affected site. In general, the most appropriate route of administration will depend upon a variety of factors including the nature of the agent (e.g., its stability in the environment of the gastrointestinal tract), and/or the condition of the subject (e.g., whether the subject is able to tolerate oral administration). 
     In some embodiments, compounds or compositions disclosed in this application are formulated and/or administered in nanoparticles. Nanoparticles are particles in the nanoscale. In some embodiments, nanoparticles are less than 1 μm in diameter. In some embodiments, nanoparticles are between about 1 and 100 nm in diameter. Nanoparticles include organic nanoparticles, such as dendrimers, liposomes, or polymeric nanoparticles. Nanoparticles also include inorganic nanoparticles, such as fullerenes, quantum dots, and gold nanoparticles. Compositions may comprise an aggregate of nanoparticles. In some embodiments, the aggregate of nanoparticles is homogeneous, while in other embodiments the aggregate of nanoparticles is heterogeneous. 
     The exact amount of a compound required to achieve an effective amount will vary from subject to subject, depending, for example, on species, age, and general condition of a subject, severity of the side effects or disorder, identity of the particular compound, mode of administration, and the like. An effective amount may be included in a single dose (e.g., single oral dose) or multiple doses (e.g., multiple oral doses). In certain embodiments, when multiple doses are administered to a subject or applied to a tissue or cell, any two doses of the multiple doses include different or substantially the same amounts of a compound described in this application. In certain embodiments, when multiple doses are administered to a subject or applied to a tissue or cell, the frequency of administering the multiple doses to the subject or applying the multiple doses to the tissue or cell is three doses a day, two doses a day, one dose a day, one dose every other day, one dose every third day, one dose every week, one dose every two weeks, one dose every three weeks, or one dose every four weeks. In certain embodiments, the frequency of administering the multiple doses to the subject or applying the multiple doses to the tissue or cell is one dose per day. In certain embodiments, the frequency of administering the multiple doses to the subject or applying the multiple doses to the tissue or cell is two doses per day. In certain embodiments, the frequency of administering the multiple doses to the subject or applying the multiple doses to the tissue or cell is three doses per day. In certain embodiments, when multiple doses are administered to a subject or applied to a tissue or cell, the duration between the first dose and last dose of the multiple doses is one day, two days, four days, one week, two weeks, three weeks, one month, two months, three months, four months, six months, nine months, one year, two years, three years, four years, five years, seven years, ten years, fifteen years, twenty years, or the lifetime of the subject, tissue, or cell. In certain embodiments, the duration between the first dose and last dose of the multiple doses is three months, six months, or one year. In certain embodiments, the duration between the first dose and last dose of the multiple doses is the lifetime of the subject, tissue, or cell. In certain embodiments, a dose (e.g., a single dose, or any dose of multiple doses) described in this application includes independently between 0.1 μg and 1 μg, between 0.001 mg and 0.01 mg, between 0.01 mg and 0.1 mg, between 0.1 mg and 1 mg, between 1 mg and 3 mg, between 3 mg and 10 mg, between 10 mg and 30 mg, between 30 mg and 100 mg, between 100 mg and 300 mg, between 300 mg and 1,000 mg, or between 1 g and 10 g, inclusive, of a compound described in this application. In certain embodiments, a dose described in this application includes independently between 1 mg and 3 mg, inclusive, of a compound described in this application. In certain embodiments, a dose described in this application includes independently between 3 mg and 10 mg, inclusive, of a compound described in this application. In certain embodiments, a dose described in this application includes independently between 10 mg and 30 mg, inclusive, of a compound described in this application. In certain embodiments, a dose described in this application includes independently between 30 mg and 100 mg, inclusive, of a compound described in this application. 
     Dose ranges as described in this application provide guidance for the administration of provided pharmaceutical compositions to an adult. The amount to be administered to, for example, a child or an adolescent can be determined by a medical practitioner or person skilled in the art and can be lower or the same as that administered to an adult. 
     A compound or composition, as described in this application, can be administered in combination with one or more additional pharmaceutical agents (e.g., therapeutically and/or prophylactically active agents). The compounds or compositions can be administered in combination with additional pharmaceutical agents that improve their activity, improve bioavailability, improve safety, reduce drug resistance, reduce and/or modify metabolism, inhibit excretion, and/or modify distribution in a subject or cell. It will also be appreciated that the therapy employed may achieve a desired effect for the same disorder, and/or it may achieve different effects. In certain embodiments, a pharmaceutical composition described in this application including a compound described in this application and an additional pharmaceutical agent shows a synergistic effect that is absent in a pharmaceutical composition including one of the compound and the additional pharmaceutical agent, but not both. 
     The compound or composition can be administered concurrently with, prior to, or subsequent to one or more additional pharmaceutical agents, which may be useful as, e.g., combination therapies. Pharmaceutical agents include therapeutically active agents. Pharmaceutical agents also include prophylactically active agents. Pharmaceutical agents include small organic molecules such as drug compounds (e.g., compounds approved for human or veterinary use by the U.S. Food and Drug Administration as provided in the Code of Federal Regulations (CFR)), peptides, proteins, carbohydrates, monosaccharides, oligosaccharides, polysaccharides, nucleoproteins, mucoproteins, lipoproteins, synthetic polypeptides or proteins, small molecules linked to proteins, glycoproteins, steroids, nucleic acids, DNAs, RNAs, nucleotides, nucleosides, oligonucleotides, antisense oligonucleotides, lipids, hormones, vitamins, and cells. In certain embodiments, the additional pharmaceutical agent is a pharmaceutical agent useful for treating and/or preventing a disease (e.g., proliferative disease, neurological disease, painful condition, psychiatric disorder, or metabolic disorder). Each additional pharmaceutical agent may be administered at a dose and/or on a time schedule determined for that pharmaceutical agent. The additional pharmaceutical agents may also be administered together with each other and/or with the compound or composition described in this application in a single dose or administered separately in different doses. The particular combination to employ in a regimen will take into account compatibility of the compound described in this application with the additional pharmaceutical agent(s) and/or the desired therapeutic and/or prophylactic effect to be achieved. In general, it is expected that the additional pharmaceutical agent(s) in combination be utilized at levels that do not exceed the levels at which they are utilized individually. In some embodiments, the levels utilized in combination will be lower than those utilized individually. 
     In some embodiments, one or more of the compositions described in this application are administered to a subject. In certain embodiments, the subject is an animal. The animal may be of either sex and may be at any stage of development. In certain embodiments, the subject is a human. In other embodiments, the subject is a non-human animal. In certain embodiments, the subject is a mammal. In certain embodiments, the subject is a non-human mammal. In certain embodiments, the subject is a domesticated animal, such as a dog, cat, cow, pig, horse, sheep, or goat. In certain embodiments, the subject is a companion animal, such as a dog or cat. In certain embodiments, the subject is a livestock animal, such as a cow, pig, horse, sheep, or goat. In certain embodiments, the subject is a zoo animal. In another embodiment, the subject is a research animal, such as a rodent (e.g., mouse, rat), dog, pig, or non-human primate. 
     Also encompassed by the disclosure are kits (e.g., pharmaceutical packs). The kits provided may comprise a composition, such as a pharmaceutical composition, or a compound described in this application and a container (e.g., a vial, ampule, bottle, syringe, and/or dispenser package, or other suitable container). In some embodiments, provided kits may optionally further include a second container comprising a pharmaceutical excipient for dilution or suspension of a pharmaceutical composition or compound described in this application. In some embodiments, the pharmaceutical composition or compound described in this application provided in the first container and the second container a combined to form one unit dosage form. 
     Thus, in one aspect, provided are kits including a first container comprising a compound or composition described in this application. In certain embodiments, the kits are useful for treating a disease in a subject in need thereof. In certain embodiments, the kits are useful for preventing a disease in a subject in need thereof. In certain embodiments, the kits are useful for reducing the risk of developing a disease in a subject in need thereof. 
     In certain embodiments, a kit described in this application further includes instructions for using the kit. A kit described in this application may also include information as required by a regulatory agency such as the U.S. Food and Drug Administration (FDA). In certain embodiments, the information included in the kits is prescribing information. In certain embodiments, the kits and instructions provide for treating a disease in a subject in need thereof. In certain embodiments, the kits and instructions provide for preventing a disease in a subject in need thereof. In certain embodiments, the kits and instructions provide for reducing the risk of developing a disease in a subject in need thereof. A kit described in this application may include one or more additional pharmaceutical agents described in this application as a separate composition. 
     In some embodiments, the compositions include consumer product, such as comestible, cosmetic, toiletry, potable, inhalable, and wellness products. Exemplary consumer products include salves, waxes, powdered concentrates, pastes, extracts, tinctures, powders, oils, capsules, skin patches, sublingual oral dose drops, mucous membrane oral spray doses, makeup, perfume, shampoos, cosmetic soaps, cosmetic creams, skin lotions, aromatic essential oils, massage oils, shaving preparations, oils for toiletry purposes, lip balm, cosmetic oils, facial washes, moisturizing creams, moisturizing body lotions, moisturizing face lotions, bath salts, bath gels, bath soaps in liquid form, shower gels, bath bombs, hair care preparations, shampoos, conditioner, chocolate bars, brownies, chocolates, cookies, crackers, cakes, cupcakes, puddings, honey, chocolate confections, frozen confections, fruit-based confectionery, sugar confectionery, gummy candies, dragées, pastries, cereal bars, chocolate, cereal based energy bars, candy, ice cream, tea-based beverages, coffee-based beverages, and herbal infusions. 
     The present invention is further illustrated by the following Examples, which in no way should be construed as limiting. The entire contents of all of the references (including literature references, issued patents, published patent applications, and co-pending patent applications) cited throughout this application are hereby expressly incorporated by reference. If a reference incorporated in this application contains a term whose definition is incongruous or incompatible with the definition of same term as defined in the present disclosure, the meaning ascribed to the term in this disclosure shall govern. However, mention of any reference, article, publication, patent, patent publication, and patent application cited in this application is not, and should not be taken as an acknowledgment or any form of suggestion that they constitute valid prior art or form part of the common general knowledge in any country in the world. 
     Examples 
     Example 1: Primary High-Throughput Screen to Identify Functional Expression of 
     Cannabichromenic Acid Synthases (CBCASs) 
     To identify CBCAS genes that can be functionally expressed in host cells, a library of approximately 3000 candidate CBCAS genes was designed based on internal codebases and domain knowledge, sampled across enzyme families, ecological niches, and structural homologies. Protein sequences were recoded in silico for expression in  S. cerevisiae  and synthesized in the integrative yeast expression vector shown in  FIG.  5   . Each candidate enzyme expression construct was transformed into an  S. cerevisiae  CEN.PK strain that also expressed a prenyltransferase enzyme capable of catalyzing reaction R 4  in  FIG.  2   . Strain t616313, expressing GFP, was included in the library screen as a negative control for enzyme activity. 
     A putative  C. sativa  CBCAS enzyme that was previously disclosed was not found to be active. Instead, a  C. sativa  THCAS enzyme (set forth in SEQ ID NO:23) was found to demonstrate CBCAS activity in addition to THCAS activity using the assays described in this Example, and was accordingly used as a positive control for CBCAS activity (strain t616315). All candidate enzymes in the library, as well as the enzyme expressed by positive control strain t616315, included an N-terminal MFalpha2 signal peptide (SEQ ID NO: 16), (with a methionine residue added at the N-terminus of the MFalpha2 signal peptide), and a C-terminal HDEL signal peptide (SEQ ID NO: 17). 
     An assay to detect TS activity was conducted as follows: each thawed glycerol stock of candidate CBCAS transformants was stamped into a well of YEP+4% dextrose media. Samples were incubated at 30° C. in a shaking incubator for 2 days. A portion of each of the resulting cultures was stamped into a well of YEP+4% galactose+1 mM olivetolic acid ( FIG.  1    Structure 6a). Samples were incubated at 20° C. and shaken in a shaking incubator for 4 days. Every 24 hours during those 4 days, 2% galactose and 1 mM olivetolic acid were spiked into the cultures. Sodium citrate buffer adjusted to pH 5.5 was added to each well at a final concentration of 100 mM. Samples were incubated at 20° C. and shaken in a shaking incubator for 2 days. A portion of each of the resulting production cultures was stamped into a well of phosphate buffered saline (PBS). Optical measurements were taken on a plate reader, with absorbance measured at 600 nm and fluorescence at 528 nm with 485 nm excitation. Samples were incubated at 30° C. in a shaking incubator for 2 days. 100% methanol was stamped into the production cultures in half-height deepwell plates. Plates were heat sealed and frozen. Samples were then thawed for 30 min and spun down at 4° C. A portion of the supernatant was stamped into half-area 96 well plates. CBCA, THCA, and CBDA production in the samples was quantified via liquid chromatography-mass spectrometry (LC-MS). 
     The library of candidate CBCAS enzymes was assayed for activity in a primary high-throughput screen using the assay described above. LC-MS analysis revealed a single “hit” CBCAS (strain t619896, expressing an  A. niger  protein of SEQ ID NO: 25 linked to an N-terminal MFalpha2 signal peptide (with a methionine residue added at the N-terminus of the MFalpha2 signal peptide) and a C-terminal HDEL signal peptide), that produced measurable amounts of CBCA. 
     Surprisingly, the candidate  A. niger  CBCAS enzyme has very low sequence identity with  C. sativa  CBCAS and THCAS enzymes. An alignment of the  A. niger  CBCAS enzyme (SEQ ID NO: 27 (UniProt accession No. A0A254UC34), which corresponds to SEQ ID NO: 25 plus a methionine residue at the N-terminus) with a putative  C. sativa  CBCAS enzyme (SEQ ID NO: 15), and a  C. sativa  THCAS enzyme (SEQ ID NO: 20, corresponding to UniProt accession No. I1V0C5) using BLASTP with default parameters, reveals 21.15% identity, and 21.71% identity, respectively. 
     To confirm the activity of the candidate CBCAS enzyme identified in the primary screen, a secondary screen was performed to verify CBCA production. The experimental protocol for the secondary screen was identical to the primary screen, except that additional biological replicates were included per strain, and replicate production cultures for each strain were separately fed 1 mM olivetolic acid or 1 mM divaric acid. All strains were screened in quadruplicate. 
     Consistent with the primary screen, the secondary screen revealed CBCAS activity for strain t619896, as shown by titers of CBCA produced by this strain (Table 5 and  FIG.  6   ). 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 CBCA titers from secondary screening of CBCAS 
               
               
                 candidate enzymes in  S. cerevisiae   
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Average 
                 Standard 
               
               
                   
                   
                 CBCA 
                 Deviation CBCA 
               
               
                 Strain 
                 Strain type 
                 [μg/L] 
                 [μg/L] 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 t616313 
                 Negative Control (GFP) 
                 0.0 
                 0.0 
               
               
                 t616315 
                 Positive Control 
                 362.9 
                 575.6 
               
               
                   
                 ( C. sativa  THCAS) 
               
               
                 t619896 
                 Library 
                 13772.4 
                 978.5 
               
               
                   
                 ( A. niger  CBCAS) 
               
               
                   
               
            
           
         
       
     
     Surprisingly, strain t619896 also revealed CBCVAS activity, as shown by titers of CBCVA produced by this strain (Table 6 and  FIG.  7   ). Strain t616315, which was used as a positive control for production of CBCA in the secondary screen, did not demonstrate CBCVAS activity (Table 6 and  FIG.  7   ). 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 CBCVA titers from secondary screening of CBCAS 
               
               
                 candidate enzymes in  S. cerevisiae   
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Average 
                 Standard 
               
               
                   
                   
                 CBCVA 
                 Deviation CBCVA 
               
               
                 Strain 
                 Strain type 
                 [μg/L] 
                 [μg/L] 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 t616313 
                 Negative Control (GFP) 
                 0 
                 0 
               
               
                 t616315 
                 Positive Control 
                 0 
                 0 
               
               
                   
                 ( C. sativa  THCAS) 
               
               
                 t619896 
                 Library 
                 2609.3 
                 602.5 
               
               
                   
                 ( A. niger  CBCAS) 
               
               
                   
               
            
           
         
       
     
     Strain t619896 also demonstrated production of THCA and CBDA, producing a terminal cannabinoid product profile consisting of 89.60% CBCA, 5.67% CBDA, and 4.73% THCA (Table 7). 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 CBCA, THCA, and CBDA titers from secondary screening of CBCAS candidate enzymes in  S. cerevisiae   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                 Standard 
                   
                 Standard 
                   
                 Standard 
                   
                   
                   
               
               
                   
                   
                 Average 
                 Deviation 
                 Average 
                 Deviation 
                 Average 
                 Deviation 
               
               
                 Strain 
                 Strain 
                 CBCA 
                 CBCA 
                 THCA 
                 THCA 
                 CBDA 
                 CBDA 
                 % 
                 % 
                 % 
               
               
                 ID 
                 Type 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 CBCA 
                 THCA 
                 CBDA 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 t616313 
                 Negative 
                 0.00 
                 0.00 
                 506.91 
                 1467.67 
                 6.89 
                 20.62 
                 0.00 
                 98.66 
                 1.34 
               
               
                   
                 Control 
               
               
                   
                 (GFP) 
               
               
                 t616314 
                 Positive 
                 47.51 
                 68.16 
                 433.82 
                 1844.40 
                 719.89 
                 371.17 
                 3.95 
                 36.12 
                 59.93 
               
               
                   
                 Control 
               
               
                   
                 ( C. sativa   
               
               
                   
                 CBDAS) 
               
               
                 t616315 
                 Positive 
                 362.95 
                 575.63 
                 19030.65 
                 13680.86 
                 142.10 
                 169.23 
                 1.86 
                 97.41 
                 0.73 
               
               
                   
                 Control 
               
               
                   
                 ( C. sativa   
               
               
                   
                 THCAS) 
               
               
                 t619896 
                 Library 
                 13772.43 
                 978.55 
                 727.30 
                 71.49 
                 872.03 
                 158.52 
                 89.60 
                 4.73 
                 5.67 
               
               
                   
                 ( A. niger   
               
               
                   
                 CBCAS) 
               
               
                   
               
            
           
         
       
     
     Thus, out of approximately 3000 candidate genes, one CBCAS was surprisingly identified as being able to produce measurable amounts of CBCA and CBCVA when expressed in  S. cerevisiae  host cells. The CBCAS identified in these screens may be useful in cannabinoid biosynthesis. 
     Example 2: Protein Engineering of  A. niger  CBCAS 
     To determine whether engineering of the  A. niger  CBCAS identified in Example 1 (corresponding to SEQ ID NO: 29 (with signal peptides); SEQ ID NO: 27 (without signal peptides and including an N-terminal methionine (UniProt accession No. A0A254UC34)); or SEQ ID NO: 25 (without signal peptides and without the N-terminal methionine)), could alter CBCAS substrate specificity, product specificity and/or amounts of products produced, point mutations were generated in  A. niger  CBCAS and the mutant versions of the protein were expressed in  S. cerevisiae . A library containing 1047  A. niger  CBCAS mutants was generated and screened. As in Example 1, each CBCAS mutant in the library, as well as the enzymes expressed by positive control strains, included an N-terminal MFalpha2 signal peptide (SEQ ID NO: 16) (with a methionine residue added at the N-terminus of the MFalpha2 signal peptide) and a C-terminal HDEL signal peptide (SEQ ID NO: 17). 
     Production of compounds of Formulae (9), (10), and/or (11), including compounds of Formulae (9a), (10a), and/or (11a) by strains expressing the mutated versions of  A. niger  CBCAS was quantified and compared to the production of the same compounds by a strain expressing wild-type  A. niger  CBCAS, a strain expressing a  C. sativa  THCAS, and a strain expressing a  C. sativa  CBDAS. The strains were screened using the same assay described in Example 1. Production of CBCA, THCA, and/or CBDA in the samples was quantified via LC-MS. 
     Of the original 1047 library members, 55 strains were elevated to a secondary screen to verify CBCA production. The experimental protocol for the secondary screen was identical to the primary screen, except that additional biological replicates were included per strain, and replicate production cultures for each strain were separately fed 1 mM boluses of olivetolic acid or 1 mM boluses of divaric acid. All strains were screened in quadruplicate. 
     Of the 55 strains assessed in the secondary screen, 21 demonstrated a higher average CBCA titer than the  A. niger  positive control, including: strain t878470, which expresses a mutant version of  A. niger  CBCAS containing A57Q and G61A point mutations relative to SEQ ID NO: 27; strain t865743, which expresses a mutant version of  A. niger  CBCAS containing a V260M mutation relative to SEQ ID NO: 27; strain t865737, which expresses a mutant version of  A. niger  CBCAS containing a V62I mutation relative to SEQ ID NO: 27; strain t865746, which expresses a mutant version of  A. niger  CBCAS containing a V386A mutation relative to SEQ ID NO: 27; strain t865744, which expresses a mutant version of  A. niger  CBCAS containing a V260F mutation relative to SEQ ID NO: 27; strain t865717, which expresses a mutant version of  A. niger  CBCAS containing E112V and N122S point mutations relative to SEQ ID NO: 27; strain t865694, which expresses a mutant version of  A. niger  CBCAS containing A57E and I126A point mutations relative to SEQ ID NO: 27; strain t865726, which expresses a mutant version of  A. niger  CBCAS containing T33D and N257S point mutations relative to SEQ ID NO: 27; strain t878465, which expresses a mutant version of  A. niger  CBCAS containing N202S and P472A point mutations relative to SEQ ID NO: 27; strain t865771, which expresses a mutant version of  A. niger  CBCAS containing a D410N point mutation relative to SEQ ID NO: 27; strain t865739, which expresses a mutant version of  A. niger  CBCAS containing a R450K point mutation relative to SEQ ID NO: 27; strain t865750, which expresses a mutant version of  A. niger  CBCAS containing a S180T point mutation relative to SEQ ID NO: 27; strain t878464, which expresses a mutant version of  A. niger  CBCAS containing a R183T point mutation relative to SEQ ID NO: 27; strain t865689, which expresses a mutant version of  A. niger  CBCAS containing N122G and I126R point mutations relative to SEQ ID NO: 27; strain t865690, which expresses a mutant version of  A. niger  CBCAS containing N122A and I126T point mutations relative to SEQ ID NO: 27; strain t865749, which expresses a mutant version of  A. niger  CBCAS containing a Y71I point mutation relative to SEQ ID NO: 27; strain t865728, which expresses a mutant version of  A. niger  CBCAS containing H287R and A341S point mutations relative to SEQ ID NO: 27; strain t865805, which expresses a mutant version of  A. niger  CBCAS containing T55S and 1126T point mutations relative to SEQ ID NO: 27; strain t865711, which expresses a mutant version of  A. niger  CBCAS containing N122G and V398F point mutations relative to SEQ ID NO: 27; strain t865714, which expresses a mutant version of  A. niger  CBCAS containing a M394T point mutation relative to SEQ ID NO: 27; and strain t865729, which expresses a mutant version of  A. niger  CBCAS containing A57E and N131S point mutations relative to SEQ ID NO: 27. ( FIG.  8 A ; Table 8.) 
     Surprisingly these 21 mutant CBCAS hits also demonstrated enhanced product specificity for CBCA. For example, the  A. niger  positive control produced a terminal cannabinoid product profile consisting of 73.74% CBCA, 21.55% CBDA, and 4.72% THCA, whereas certain CBCAS mutants were identified that produced more than 80% CBCA (80-83% CBCA, 13-14% CBDA, and 3-5% THCA). 
     Of the 55 strains assessed in the secondary screen, 24 demonstrated a higher average CBCVA titer than the  A. niger  positive control, including: strain t865745, which expresses a mutant version of  A. niger  CBCAS containing a V63I point mutation relative to SEQ ID NO: 27; strain t865689, which expresses a mutant version of  A. niger  CBCAS containing N122G and I126R point mutations relative to SEQ ID NO: 27; strain t865718, which expresses a mutant version of  A. niger  CBCAS containing a P472R point mutation relative to SEQ ID NO: 27; strain t865750, which expresses a mutant version of  A. niger  CBCAS containing a S180T point mutation relative to SEQ ID NO: 27; strain t865747, which expresses a mutant version of  A. niger  CBCAS containing a V398A point mutation relative to SEQ ID NO: 27; strain t878464, which expresses a mutant version of  A. niger  CBCAS containing a R183T point mutation relative to SEQ ID NO: 27; strain t865743, which expresses a mutant version of  A. niger  CBCAS containing a V260M point mutation relative to SEQ ID NO: 27; strain t865746, which expresses a mutant version of  A. niger  CBCAS containing a V386A point mutation relative to SEQ ID NO: 27; strain t865732, which expresses a mutant version of  A. niger  CBCAS containing a H426Y point mutation relative to SEQ ID NO: 27; strain t865741, which expresses a mutant version of  A. niger  CBCAS containing a Y256M point mutation relative to SEQ ID NO: 27; strain t878465, which expresses a mutant version of  A. niger  CBCAS containing N202S and P472A point mutations relative to SEQ ID NO: 27; strain t865720, which expresses a mutant version of  A. niger  CBCAS containing N122G and I126K point mutations relative to SEQ ID NO: 27; strain t865737, which expresses a mutant version of  A. niger  CBCAS containing a V62I point mutation relative to SEQ ID NO: 27; strain t865739, which expresses a mutant version of  A. niger  CBCAS containing a R450K point mutation relative to SEQ ID NO: 27; strain t865723, which expresses a mutant version of  A. niger  CBCAS containing a Y129W point mutation relative to SEQ ID NO: 27; strain t865751, which expresses a mutant version of  A. niger  CBCAS containing a S423A point mutation relative to SEQ ID NO: 27; strain t865728, which expresses a mutant version of  A. niger  CBCAS containing H287R and A341S point mutations relative to SEQ ID NO: 27; strain t865736, which expresses a mutant version of  A. niger  CBCAS containing a N295S point mutation relative to SEQ ID NO: 27; strain t865748, which expresses a mutant version of  A. niger  CBCAS containing a Y39F point mutation relative to SEQ ID NO: 27; strain t865744, which expresses a mutant version of  A. niger  CBCAS containing a V260F point mutation relative to SEQ ID NO: 27; strain t865755, which expresses a mutant version of  A. niger  CBCAS containing a L392H point mutation relative to SEQ ID NO: 27; strain t865729, which expresses a mutant version of  A. niger  CBCAS containing A57E and N131S point mutations relative to SEQ ID NO: 27; strain t865717, which expresses a mutant version of  A. niger  CBCAS containing E112V and N122S point mutations relative to SEQ ID NO: 27; and strain t865726, which expresses a mutant version of  A. niger  CBCAS containing T33D and N257S point mutations relative to SEQ ID NO: 27. ( FIG.  9 A ; Table 9.) Unlike for the hits identified on olivetolic acid, a shift in product profile was not observed among the terminal cannabinoids produced from divaric acid. Rather, this product profile was 67-70% CBCVA and 30-33% THCVA for both the  A. niger  control and the mutant hits. Surprisingly CBDVA was not observed among the products generated by the CBCAS candidates assessed in this screen. 
     Multiple library strains were observed to produce THCA and THCVA. Strain t865768, expressing the  A. niger  CBCAS produced a higher average THCA titer than the positive control THCAS strain ( FIG.  8 B ; Table 8.). Additionally, 33 library strains expressing  A. niger  CBCAS mutants produced a higher average THCA titer than the positive control THCAS strain ( FIG.  8 B ; Table 8.) Additionally, Strain t865768, expressing the  A. niger  CBCAS, and most of the tested library strains expressing  A. niger  CBCAS mutants produced more THCVA than the positive control THCAS strain ( FIG.  9 B ; Table 9.) 
     Multiple library strains were also observed to produce CBDA. Strain t865768, expressing the  A. niger  CBCAS and most of the tested library strains expressing  A. niger  CBCAS mutants produced more CBDA than the positive control CBDAS strain (t876607), which expressed a  Cannabis  CBDAS. Consistent with previous reports (Luo et al. Nature, 2019 March; 567(7746):123-126), the  Cannabis  CBDAS has low to no activity in a  S. cerevisiae  host cell: ( FIG.  8 C ; Table 8). No library strains tested were found to produce CBDVA ( FIG.  9 C ; Table 9). 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 CBCA, THCA, and CBDA titers from protein engineering of CBCAS candidate enzymes in  S. cerevisiae   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 Strain type/ 
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 Point mutations 
                 Mean 
                 Std Dev. 
                 Mean 
                 Std Dev. 
                 Mean 
                 Std Dev 
               
               
                 Strain 
                 relative to 
                 CBCA 
                 CBCA 
                 THCA 
                 THCA 
                 CBDA 
                 CBDA 
                 % 
                 % 
                 % 
               
               
                 ID 
                 SEQ ID NO: 27 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 CBCA 
                 THCA 
                 CBDA 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 t865768 
                   A. niger  CBCAS 
                 31539.55 
                 2195.41 
                 2016.94 
                 224.36 
                 9216.26 
                 1477.71 
                 73.74 
                 4.72 
                 21.55 
               
               
                   
                 Positive Control 
               
               
                 t865843 
                 THCAS 
                 0 
                 0 
                 1681.35 
                 1025.75 
                 0 
                 0 
                 0.00 
                 100.00 
                 0.00 
               
               
                   
                 Positive Control 
               
               
                 t876607 
                 CBDAS 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0.00 
                 0.00 
                 0.00 
               
               
                   
                 Positive Control 
               
               
                 t865842 
                 GFP 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
               
               
                   
                 Negative Control 
               
               
                 t878470 
                 Library/A57Q 
                 64502.70 
                 42097.39 
                 2739.24 
                 1708.61 
                 10538.52 
                 3890.08 
                 82.93 
                 3.52 
                 13.55 
               
               
                   
                 G61A 
               
               
                 t865743 
                 Library/V260M 
                 58061.40 
                 14603.74 
                 3389.10 
                 103.61 
                 11245.27 
                 3070.56 
                 79.87 
                 4.66 
                 15.47 
               
               
                 t865737 
                 Library/V62I 
                 53771.53 
                 39388.63 
                 2873.74 
                 1195.99 
                 10699.35 
                 3847.33 
                 79.85 
                 4.27 
                 15.89 
               
               
                 t865746 
                 Library/V386A 
                 49195.03 
                 11206.00 
                 2882.16 
                 432.15 
                 9456.09 
                 136.04 
                 79.95 
                 4.68 
                 15.37 
               
               
                 t865744 
                 Library/V260F 
                 44305.26 
                 4660.79 
                 2369.76 
                 461.94 
                 7187.94 
                 595.36 
                 82.26 
                 4.40 
                 13.34 
               
               
                 t865717 
                 Library/E112V 
                 44204.43 
                 9829.72 
                 2648.36 
                 760.56 
                 9698.33 
                 1430.66 
                 78.17 
                 4.68 
                 17.15 
               
               
                   
                 N122S 
               
               
                 t865694 
                 Library/A57E 
                 43506.37 
                 17223.22 
                 2579.57 
                 496.08 
                 9126.46 
                 2305.93 
                 78.80 
                 4.67 
                 16.53 
               
               
                   
                 I126A 
               
               
                 t865726 
                 Library/T33D 
                 41981.73 
                 13073.05 
                 2186.01 
                 225.25 
                 9985.90 
                 1852.88 
                 77.52 
                 4.04 
                 18.44 
               
               
                   
                 N257S 
               
               
                 t878465 
                 Library/N202S 
                 41094.07 
                 22214.68 
                 2184.77 
                 642.72 
                 9826.17 
                 4038.53 
                 77.38 
                 4.11 
                 18.50 
               
               
                   
                 P472A 
               
               
                 t865771 
                 Library/D410N 
                 40971.16 
                 11253.32 
                 2638.90 
                 350.05 
                 8309.68 
                 1295.66 
                 78.91 
                 5.08 
                 16.00 
               
               
                 t865739 
                 Library/R450K 
                 40214.21 
                 3194.26 
                 2538.89 
                 45.95 
                 10767.85 
                 1830.53 
                 75.14 
                 4.74 
                 20.12 
               
               
                 t865750 
                 Library/S180T 
                 39940.41 
                 27152.74 
                 2475.89 
                 1084.67 
                 10807.90 
                 3325.20 
                 75.04 
                 4.65 
                 20.31 
               
               
                 t878464 
                 Library/R183T 
                 38911.71 
                 16555.91 
                 2062.10 
                 1512.86 
                 9203.22 
                 3865.34 
                 77.55 
                 4.11 
                 18.34 
               
               
                 t865689 
                 Library/N122G 
                 38241.90 
                 14591.60 
                 2452.92 
                 634.89 
                 10157.65 
                 2550.20 
                 75.20 
                 4.82 
                 19.97 
               
               
                   
                 I126R 
               
               
                 t865690 
                 Library/N122A 
                 38065.26 
                 22698.56 
                 2186.36 
                 977.36 
                 8288.48 
                 2915.70 
                 78.42 
                 4.50 
                 17.08 
               
               
                   
                 I126T 
               
               
                 t865749 
                 Library/Y71I 
                 37290.06 
                 23183.06 
                 2140.74 
                 1682.07 
                 6071.00 
                 2642.28 
                 81.95 
                 4.70 
                 13.34 
               
               
                 t865728 
                 Library/H287R 
                 36875.12 
                 10430.70 
                 2692.19 
                 245.09 
                 8996.37 
                 1486.17 
                 75.93 
                 5.54 
                 18.52 
               
               
                   
                 A341S 
               
               
                 t865805 
                 Library/T55S 
                 34567.68 
                 18187.95 
                 2285.23 
                 1105.08 
                 8917.97 
                 1733.41 
                 75.52 
                 4.99 
                 19.48 
               
               
                   
                 I126T 
               
               
                 t865711 
                 Library/N122G 
                 33994.02 
                 9784.58 
                 2096.22 
                 742.86 
                 9666.71 
                 2184.88 
                 74.29 
                 4.58 
                 21.13 
               
               
                   
                 V398F 
               
               
                 t865714 
                 Library/M394T 
                 32311.22 
                 2236.43 
                 2172.74 
                 264.10 
                 6827.61 
                 1091.58 
                 78.21 
                 5.26 
                 16.53 
               
               
                 t865729 
                 Library/A57E 
                 32213.25 
                 6584.57 
                 1856.57 
                 45.07 
                 8392.06 
                 3009.17 
                 75.86 
                 4.37 
                 19.76 
               
               
                   
                 N131S 
               
               
                 t865742 
                 Library/E112T 
                 31427.04 
                 4866.15 
                 2036.13 
                 312.97 
                 9022.22 
                 1377.35 
                 73.97 
                 4.79 
                 21.24 
               
               
                   
                 N122G 
               
               
                 t865751 
                 Library/S423A 
                 31396.25 
                 16606.59 
                 1709.83 
                 731.73 
                 8775.99 
                 4271.09 
                 74.96 
                 4.08 
                 20.95 
               
               
                 t865724 
                 Library/T102N 
                 30758.44 
                 22610.92 
                 2146.00 
                 1745.90 
                 7663.56 
                 4141.79 
                 75.82 
                 5.29 
                 18.89 
               
               
                   
                 V114T 
               
               
                 t865718 
                 Library/P472R 
                 28669.67 
                 11079.11 
                 1640.20 
                 565.38 
                 7340.65 
                 2480.50 
                 76.15 
                 4.36 
                 19.50 
               
               
                 t865745 
                 Library/V63I 
                 27923.31 
                 10753.63 
                 1963.25 
                 745.90 
                 7360.14 
                 4485.51 
                 74.97 
                 5.27 
                 19.76 
               
               
                 t865720 
                 Library/N122G 
                 27895.13 
                 8460.02 
                 1543.57 
                 181.34 
                 7663.58 
                 4035.53 
                 75.18 
                 4.16 
                 20.66 
               
               
                   
                 I126K 
               
               
                 t865730 
                 Library/N202G 
                 27874.45 
                 13102.29 
                 1771.94 
                 885.27 
                 8385.38 
                 3086.02 
                 73.29 
                 4.66 
                 22.05 
               
               
                   
                 H466N 
               
               
                 t865735 
                 Library/T446P 
                 27519.94 
                 94.67 
                 1783.72 
                 69.10 
                 7436.20 
                 1564.24 
                 74.90 
                 4.85 
                 20.24 
               
               
                   
                 H466N 
               
               
                 t878468 
                 Library/A57E 
                 26823.18 
                 6838.86 
                 1922.88 
                 150.52 
                 8556.40 
                 711.58 
                 71.91 
                 5.15 
                 22.94 
               
               
                   
                 T102Q 
               
               
                 t865692 
                 Library/A57E 
                 26625.20 
                 10692.03 
                 1712.61 
                 326.02 
                 7293.40 
                 2324.07 
                 74.72 
                 4.81 
                 20.47 
               
               
                   
                 T102S 
               
               
                 t865758 
                 Library/E456A 
                 26316.76 
                 980.84 
                 1712.53 
                 710.87 
                 6998.62 
                 1073.23 
                 75.13 
                 4.89 
                 19.98 
               
               
                   
                 H466N 
               
               
                 t865736 
                 Library/N295S 
                 24918.92 
                 14722.56 
                 1690.72 
                 597.82 
                 6931.79 
                 2177.76 
                 74.29 
                 5.04 
                 20.67 
               
               
                 t865734 
                 Library/A57E 
                 24880.40 
                 10047.91 
                 1632.06 
                 905.04 
                 6677.34 
                 3006.55 
                 74.96 
                 4.92 
                 20.12 
               
               
                   
                 G61A 
               
               
                 t865795 
                 Library/F262I 
                 24874.10 
                 11028.22 
                 1807.99 
                 837.97 
                 7028.82 
                 2643.85 
                 73.79 
                 5.36 
                 20.85 
               
               
                 t878466 
                 Library/Q161K 
                 23882.09 
                 8907.36 
                 1649.00 
                 361.30 
                 8030.74 
                 3022.45 
                 71.16 
                 4.91 
                 23.93 
               
               
                 t865723 
                 Library/Y129W 
                 22893.08 
                 15795.28 
                 1788.74 
                 1346.84 
                 7334.92 
                 4905.12 
                 71.50 
                 5.59 
                 22.91 
               
               
                 t865732 
                 Library/H426Y 
                 22672.81 
                 14284.27 
                 1523.15 
                 807.78 
                 6720.79 
                 3752.27 
                 73.34 
                 4.93 
                 21.74 
               
               
                 t865696 
                 Library/N122G 
                 21496.89 
                 3186.39 
                 1567.45 
                 49.69 
                 6820.56 
                 218.82 
                 71.93 
                 5.24 
                 22.82 
               
               
                   
                 G469S 
               
               
                 t865748 
                 Library/Y39F 
                 21260.42 
                 3672.95 
                 1575.92 
                 19.15 
                 6161.32 
                 386.21 
                 73.32 
                 5.43 
                 21.25 
               
               
                 t865721 
                 Library/Y256F 
                 21099.41 
                 1743.97 
                 1396.80 
                 220.29 
                 5260.35 
                 544.40 
                 76.02 
                 5.03 
                 18.95 
               
               
                 t865809 
                 Library/N122G 
                 20413.40 
                 971.41 
                 1390.36 
                 1.49 
                 6738.75 
                 219.63 
                 71.52 
                 4.87 
                 23.61 
               
               
                   
                 I126D 
               
               
                 t865814 
                 Library/D280N 
                 20192.93 
                 3941.32 
                 1367.48 
                 249.49 
                 6751.44 
                 1040.07 
                 71.32 
                 4.83 
                 23.85 
               
               
                 t865796 
                 Library/L458W 
                 19975.57 
                 898.91 
                 1436.09 
                 62.66 
                 6427.56 
                 744.81 
                 71.75 
                 5.16 
                 23.09 
               
               
                 t865755 
                 Library/L392H 
                 19432.21 
                 13347.17 
                 1001.22 
                 1415.94 
                 4507.57 
                 3615.50 
                 77.91 
                 4.01 
                 18.07 
               
               
                 t865733 
                 Library/V398T 
                 18070.99 
                 6701.89 
                 1307.71 
                 675.32 
                 6320.33 
                 2190.16 
                 70.32 
                 5.09 
                 24.59 
               
               
                   
                 H466N 
               
               
                 t865747 
                 Library/V398A 
                 18021.77 
                 4484.44 
                 1188.04 
                 471.71 
                 4783.24 
                 1417.67 
                 75.11 
                 4.95 
                 19.94 
               
               
                 t865725 
                 Library/N122G 
                 17948.56 
                 2644.64 
                 1197.84 
                 184.04 
                 6120.73 
                 541.65 
                 71.04 
                 4.74 
                 24.22 
               
               
                   
                 I126A 
               
               
                 t865727 
                 Library/H353A 
                 16276.39 
                 7210.82 
                 1177.87 
                 281.84 
                 4297.38 
                 938.33 
                 74.83 
                 5.42 
                 19.76 
               
               
                   
                 E456A 
               
               
                 t865731 
                 Library/V25A 
                 16059.53 
                 10389.40 
                 971.80 
                 1374.33 
                 4006.74 
                 838.84 
                 76.34 
                 4.62 
                 19.05 
               
               
                   
                 L43I 
               
               
                 t865741 
                 Library/Y256M 
                 15982.40 
                 1243.85 
                 957.33 
                 44.81 
                 4419.64 
                 132.81 
                 74.83 
                 4.48 
                 20.69 
               
               
                 t865740 
                 Library/N122E 
                 11837.41 
                 10494.74 
                 685.53 
                 969.48 
                 3671.56 
                 5192.38 
                 73.10 
                 4.23 
                 22.67 
               
               
                   
                 V398L 
               
               
                 t865772 
                 Library/D35A 
                 9992.22 
                 8522.88 
                 622.72 
                 880.66 
                 3051.02 
                 4314.80 
                 73.12 
                 4.56 
                 22.33 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 CBCVA, THCVA, and CBDVA titers from protein engineering of CBCAS candidate enzymes in  S. cerevisiae   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 Strain type/ 
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 Point mutations 
                 Mean 
                 Std Dev. 
                 Mean 
                 Std Dev. 
                 Mean 
                 Std Dev. 
               
               
                 Strain 
                 relative to 
                 CBCVA 
                 CBCVA 
                 THCVA 
                 THCVA 
                 CBDVA 
                 CBDVA 
                 % 
                 % 
                 % 
               
               
                 ID 
                 SEQ ID NO: 27 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 CBCVA 
                 THCVA 
                 CBDVA 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 t865768 
                   A. niger  CBCAS 
                 3642.91 
                 1964.14 
                 1788.13 
                 915.18 
                 0.00 
                 0.00 
                 67.08 
                 32.92 
                 0.00 
               
               
                   
                 Positive Control 
               
               
                 t865843 
                 THCAS 
                 0 
                 0 
                 175.02 
                 350.06 
                 0 
                 0 
                 0.00 
                 100.00 
                 0.00 
               
               
                   
                 Positive Control 
               
               
                 t876607 
                 CBDAS 
                 0 
                 0 
                 0 
                 0 
                 265.53 
                 308.55 
                 0.00 
                 0.00 
                 100.00 
               
               
                   
                 Positive Control 
               
               
                 t865842 
                 GFP 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0.00 
                 0.00 
                 0.00 
               
               
                   
                 Negative Control 
               
               
                 t865745 
                 Library/V63I 
                 7068.26 
                 3144.76 
                 2991.05 
                 1315.58 
                 0.00 
                 0.00 
                 70.27 
                 29.73 
                 0.00 
               
               
                 t865689 
                 Library/N122G 
                 6333.32 
                 2138.98 
                 2791.18 
                 1019.00 
                 0.00 
                 0.00 
                 69.41 
                 30.59 
                 0.00 
               
               
                   
                 I126R 
               
               
                 t865718 
                 Library/P472R 
                 5888.44 
                 1041.48 
                 2516.89 
                 454.55 
                 0.00 
                 0.00 
                 70.06 
                 29.94 
                 0.00 
               
               
                 t865750 
                 Library/S180T 
                 5745.78 
                 1265.89 
                 2770.13 
                 539.05 
                 0.00 
                 0.00 
                 67.47 
                 32.53 
                 0.00 
               
               
                 t865747 
                 Library/V398A 
                 5571.51 
                 3965.98 
                 2154.32 
                 1459.29 
                 0.00 
                 0.00 
                 72.12 
                 27.88 
                 0.00 
               
               
                 t878464 
                 Library/R183T 
                 5383.16 
                 2382.21 
                 2710.86 
                 1113.16 
                 0.00 
                 0.00 
                 66.51 
                 33.49 
                 0.00 
               
               
                 t865743 
                 Library/V260M 
                 4972.60 
                 518.55 
                 2989.22 
                 662.39 
                 0.00 
                 0.00 
                 62.46 
                 37.54 
                 0.00 
               
               
                 t865746 
                 Library/V386A 
                 4751.98 
                 396.86 
                 2061.14 
                 73.01 
                 0.00 
                 0.00 
                 69.75 
                 30.25 
                 0.00 
               
               
                 t865732 
                 Library/H426Y 
                 4734.85 
                 2171.13 
                 2408.74 
                 994.86 
                 0.00 
                 0.00 
                 66.28 
                 33.72 
                 0.00 
               
               
                 t865741 
                 Library/Y256M 
                 4388.54 
                 2838.45 
                 2033.77 
                 1407.31 
                 0.00 
                 0.00 
                 68.33 
                 31.67 
                 0.00 
               
               
                 t878465 
                 Library/N202S 
                 4314.23 
                 902.00 
                 2144.09 
                 215.55 
                 0.00 
                 0.00 
                 66.80 
                 33.20 
                 0.00 
               
               
                   
                 P472A 
               
               
                 t865720 
                 Library/N122G 
                 4276.65 
                 2499.99 
                 2090.51 
                 1046.91 
                 0.00 
                 0.00 
                 67.17 
                 32.83 
                 0.00 
               
               
                   
                 I126K 
               
               
                 t865737 
                 Library/V62I 
                 4271.01 
                 2381.10 
                 2136.23 
                 1383.65 
                 0.00 
                 0.00 
                 66.66 
                 33.34 
                 0.00 
               
               
                 t865739 
                 Library/R450K 
                 4265.42 
                 1259.39 
                 2039.44 
                 391.72 
                 0.00 
                 0.00 
                 67.65 
                 32.35 
                 0.00 
               
               
                 t865723 
                 Library/Y129W 
                 4223.36 
                 891.21 
                 2125.21 
                 229.49 
                 0.00 
                 0.00 
                 66.52 
                 33.48 
                 0.00 
               
               
                 t865751 
                 Library/S423A 
                 3998.68 
                 626.37 
                 1894.39 
                 203.65 
                 0.00 
                 0.00 
                 67.85 
                 32.15 
                 0.00 
               
               
                 t865728 
                 Library/H287R 
                 3907.72 
                 1195.24 
                 1759.32 
                 427.70 
                 0.00 
                 0.00 
                 68.96 
                 31.04 
                 0.00 
               
               
                   
                 A341S 
               
               
                 t865736 
                 Library/N295S 
                 3847.79 
                 1905.25 
                 1963.40 
                 832.27 
                 0.00 
                 0.00 
                 66.21 
                 33.79 
                 0.00 
               
               
                 t865748 
                 Library/Y39F 
                 3759.89 
                 702.53 
                 1591.63 
                 75.81 
                 0.00 
                 0.00 
                 70.26 
                 29.74 
                 0.00 
               
               
                 t865744 
                 Library/V260F 
                 3752.20 
                 1126.84 
                 2162.61 
                 542.42 
                 0.00 
                 0.00 
                 63.44 
                 36.56 
                 0.00 
               
               
                 t865755 
                 Library/L392H 
                 3729.91 
                 1298.74 
                 1768.56 
                 500.12 
                 0.00 
                 0.00 
                 67.84 
                 32.16 
                 0.00 
               
               
                 t865729 
                 Library/A57E 
                 3685.70 
                 1033.39 
                 1839.38 
                 172.75 
                 0.00 
                 0.00 
                 66.71 
                 33.29 
                 0.00 
               
               
                   
                 N131S 
               
               
                 t865717 
                 Library/E112V 
                 3668.51 
                 73.58 
                 1721.38 
                 239.45 
                 0.00 
                 0.00 
                 68.06 
                 31.94 
                 0.00 
               
               
                   
                 N122S 
               
               
                 t865726 
                 Library/T33D 
                 3644.48 
                 1808.16 
                 1740.51 
                 652.00 
                 0.00 
                 0.00 
                 67.68 
                 32.32 
                 0.00 
               
               
                   
                 N257S 
               
               
                 t865725 
                 Library/N122G 
                 3484.40 
                 192.25 
                 1759.92 
                 87.91 
                 0.00 
                 0.00 
                 66.44 
                 33.56 
                 0.00 
               
               
                   
                 I126A 
               
               
                 t865758 
                 Library/E456A 
                 3465.87 
                 822.25 
                 1548.62 
                 269.59 
                 0.00 
                 0.00 
                 69.12 
                 30.88 
                 0.00 
               
               
                   
                 H466N 
               
               
                 t865730 
                 Library/N202G 
                 3406.05 
                 1412.56 
                 1922.88 
                 570.78 
                 0.00 
                 0.00 
                 63.92 
                 36.08 
                 0.00 
               
               
                   
                 H466N 
               
               
                 t865814 
                 Library/D280N 
                 3290.24 
                 101.52 
                 1468.01 
                 404.74 
                 0.00 
                 0.00 
                 69.15 
                 30.85 
                 0.00 
               
               
                 t865721 
                 Library/Y256F 
                 3281.34 
                 1586.08 
                 1482.54 
                 379.24 
                 0.00 
                 0.00 
                 68.88 
                 31.12 
                 0.00 
               
               
                 t878470 
                 Library/A57Q 
                 3226.77 
                 314.59 
                 1646.54 
                 2.72 
                 0.00 
                 0.00 
                 66.21 
                 33.79 
                 0.00 
               
               
                   
                 G61A 
               
               
                 t865696 
                 Library/N122G 
                 3184.90 
                 726.92 
                 1570.38 
                 334.49 
                 0.00 
                 0.00 
                 66.98 
                 33.02 
                 0.00 
               
               
                   
                 G469S 
               
               
                 t865809 
                 Library/N122G 
                 3093.25 
                 1227.36 
                 1662.32 
                 761.41 
                 0.00 
                 0.00 
                 65.04 
                 34.96 
                 0.00 
               
               
                   
                 I126D 
               
               
                 t865805 
                 Library/T55S 
                 3077.84 
                 1412.48 
                 1538.55 
                 421.80 
                 0.00 
                 0.00 
                 66.67 
                 33.33 
                 0.00 
               
               
                   
                 I126T 
               
               
                 t865694 
                 Library/A57E 
                 3069.69 
                 294.21 
                 1647.98 
                 140.28 
                 0.00 
                 0.00 
                 65.07 
                 34.93 
                 0.00 
               
               
                   
                 I126A 
               
               
                 t878466 
                 Library/Q161K 
                 2985.03 
                 62.33 
                 1623.40 
                 158.35 
                 0.00 
                 0.00 
                 64.77 
                 35.23 
                 0.00 
               
               
                 t878468 
                 Library/A57E 
                 2954.90 
                 335.09 
                 1628.92 
                 115.38 
                 0.00 
                 0.00 
                 64.46 
                 35.54 
                 0.00 
               
               
                   
                 T102Q 
               
               
                 t865735 
                 Library/T446P 
                 2900.35 
                 358.56 
                 1459.81 
                 7.23 
                 0.00 
                 0.00 
                 66.52 
                 33.48 
                 0.00 
               
               
                   
                 H466N 
               
               
                 t865742 
                 Library/E112T 
                 2864.87 
                 812.38 
                 1514.38 
                 416.60 
                 0.00 
                 0.00 
                 65.42 
                 34.58 
                 0.00 
               
               
                   
                 N122G 
               
               
                 t865692 
                 Library/A57E 
                 2649.69 
                 1065.13 
                 1366.19 
                 421.31 
                 0.00 
                 0.00 
                 65.98 
                 34.02 
                 0.00 
               
               
                   
                 T102S 
               
               
                 t865796 
                 Library/L458W 
                 2570.89 
                 328.86 
                 1344.71 
                 162.60 
                 0.00 
                 0.00 
                 65.66 
                 34.34 
                 0.00 
               
               
                 t865734 
                 Library/A57E 
                 2566.05 
                 177.20 
                 1577.56 
                 95.34 
                 0.00 
                 0.00 
                 61.93 
                 38.07 
                 0.00 
               
               
                   
                 G61A 
               
               
                 t865690 
                 Library/N122A 
                 2557.72 
                 165.88 
                 1441.19 
                 90.67 
                 0.00 
                 0.00 
                 63.96 
                 36.04 
                 0.00 
               
               
                   
                 I126T 
               
               
                 t865711 
                 Library/N122G 
                 2442.93 
                 95.92 
                 1315.45 
                 53.48 
                 0.00 
                 0.00 
                 65.00 
                 35.00 
                 0.00 
               
               
                   
                 V398F 
               
               
                 t865749 
                 Library/Y71I 
                 2230.06 
                 429.99 
                 997.07 
                 40.32 
                 0.00 
                 0.00 
                 69.10 
                 30.90 
                 0.00 
               
               
                 t865724 
                 Library/T102N 
                 2190.11 
                 1124.38 
                 1153.25 
                 541.45 
                 0.00 
                 0.00 
                 65.51 
                 34.49 
                 0.00 
               
               
                   
                 V114T 
               
               
                 t865733 
                 Library/V398T 
                 2023.09 
                 907.28 
                 1202.96 
                 424.48 
                 0.00 
                 0.00 
                 62.71 
                 37.29 
                 0.00 
               
               
                   
                 H466N 
               
               
                 t865795 
                 Library/F262I 
                 1897.16 
                 554.17 
                 1181.24 
                 377.67 
                 0.00 
                 0.00 
                 61.63 
                 38.37 
                 0.00 
               
               
                 t865727 
                 Library/H353A 
                 1829.32 
                 696.52 
                 981.31 
                 223.32 
                 0.00 
                 0.00 
                 65.09 
                 34.91 
                 0.00 
               
               
                   
                 E456A 
               
               
                 t865714 
                 Library/M394T 
                 1775.08 
                 353.96 
                 1101.76 
                 302.87 
                 0.00 
                 0.00 
                 61.70 
                 38.30 
                 0.00 
               
               
                 t865731 
                 Library/V25A 
                 1605.94 
                 368.12 
                 885.33 
                 26.61 
                 0.00 
                 0.00 
                 64.46 
                 35.54 
                 0.00 
               
               
                   
                 L43I 
               
               
                 t865771 
                 Library/D410N 
                 1592.02 
                 388.99 
                 968.82 
                 349.88 
                 0.00 
                 0.00 
                 62.17 
                 37.83 
                 0.00 
               
               
                 t865772 
                 Library/D35A 
                 1441.55 
                 2038.66 
                 702.24 
                 993.12 
                 0.00 
                 0.00 
                 67.24 
                 32.76 
                 0.00 
               
               
                 t865740 
                 Library/N122E 
                 1153.83 
                 483.47 
                 469.98 
                 664.66 
                 0.00 
                 0.00 
                 71.06 
                 28.94 
                 0.00 
               
               
                   
                 V398L 
               
               
                   
               
            
           
         
       
     
     Example 3: High-Throughput Screen to Identify Metagenomic Cannabichromenic Acid Synthases (CRCASs) 
     To our knowledge the CBCAS from  A. niger  identified in Example 1 represents the first enzyme possessing this activity to be discovered outside of the  Cannabis  genus. To explore whether other putative CBCASs may exist in the broader metagenome, a library of 1072 candidate CBCAS genes was designed using the  A. niger  CBCAS enzyme identified in Example 1 as a reference. Protein sequences were recoded in silico for expression in  S. cerevisiae  and synthesized in the integrative yeast expression vector shown in  FIG.  5   . Each candidate enzyme expression construct was transformed into an  S. cerevisiae  CEN.PK strain that also expressed a prenyltransferase enzyme capable of catalyzing reaction R 4  in  FIG.  2   . Strain t616313, expressing GFP, was included in the library screen as a negative control for enzyme activity. Strain t807925, expressing the  A. niger  enzyme identified in Example 1, was included in the library screen as a positive control for enzyme activity. All candidate enzymes in the library, as well as the enzyme expressed by positive control strain t807925, included an N-terminal MFalpha2 signal peptide (SEQ ID NO: 16) (with a methionine residue added at the N-terminus of the MFalpha2 signal peptide) and a C-terminal HDEL signal peptide (SEQ ID NO: 17). 
     The library of candidate CBCAS enzymes was assayed for activity in a primary high-throughput screen using the assay described in Example 1. Production of CBCA, THCA, and/or CBDA in the samples was quantified via LC-MS. 
     Based on results of the primary screen, 70 strains were carried forward to a secondary screen to confirm activity observed in the primary screen. The experimental protocol for the secondary screen was identical to the primary screen, except that additional technical replicates were included per strain, and replicate production cultures for each strain were separately fed 1 mM olivetolic acid or 1 mM divaric acid. All strains were screened in quadruplicate ( FIGS.  10 A- 10 C , Tables 10 and 11). Strain IDs and their corresponding sequences are shown in Table 15. 
     These results surprisingly identified multiple strains that are capable of producing CBCA and/or CBCVA. Specifically, 17 strains produced amounts of CBCA comparable to amounts produced by the positive control (corresponding to a mean CBCA titer at least within 1 standard deviation of the mean CBCA titer of strain t807925) while 2 strains (t808223 and t808199) produced CBCA at a titer of more than 1 standard deviation of the mean CBCA titer of strain t807925 ( FIG.  10 A ). 28 strains demonstrated comparable CBCVAS activity to the positive control ( FIG.  11 A ). Of these 17 strains, multiple strains, including: t807854—SEQ ID NO: 112, t807933—SEQ ID NO: 130, t808225—SEQ ID NO: 166, t808026—SEQ ID NO: 144, and t8082001—SEQ ID NO: 164 produced a terminal cannabinoid product profile with a higher percentage of CBCA than the  A. niger  positive control, with 1 strain (t807854—SEQ ID NO: 112) producing terminal cannabinoid products with a profile of over 97% CBCA. 
     A subset of candidate CBCASs was identified that exhibited &gt;95% sequence identity to the  A. niger  CBCAS identified in Example 1 ( FIG.  13   ). 
     It was observed that several strains that produced CBCA and/or CBCVA completely exhausted their respective substrate (e.g., CBGA or CBGVA) ( FIGS.  12 A- 12 B , Table 12). Accordingly, while multiple strains were identified that are capable of producing CBCA and/or CBCVA, the observed substrate exhaustion precludes effective ranking between the strains based on production of CBCA. 
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 CBCA, THCA, and CBDA titers from metagenomic screening of CBCAS candidate enzymes in  S. cerevisiae   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                 Mean 
                 Std Dev. 
                 Mean 
                 Std Dev. 
                 Mean 
                 Std Dev 
                   
                   
                   
               
               
                 Strain 
                 Strain 
                 TS SEQ 
                 CBCA 
                 CBCA 
                 THCA 
                 THCA 
                 CBDA 
                 CBDA 
                 % 
                 % 
                 % 
               
               
                 ID 
                 Type 
                 ID NO* 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 CBCA 
                 THCA 
                 CBDA 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 t807925 
                   A. niger  CBCAS 
                  27 
                 26702.23 
                 3170.88 
                 1248.46 
                 146.74 
                 59.53 
                 81.81 
                 95.33 
                 4.46 
                 0.21 
               
               
                   
                 Positive Control 
               
               
                 t616313 
                 GFP 
                 — 
                 0 
                 0 
                 103.88 
                 293.83 
                 0 
                 0 
                 0.00 
                 100.00 
                 0.00 
               
               
                   
                 Negative Control 
               
               
                 t616314 
                 CBDAS 
                 — 
                 60.45 
                 170.99 
                 0.00 
                 0.00 
                 1170.28 
                 150.50 
                 4.91 
                 0.00 
                 95.09 
               
               
                   
                 Positive Control 
               
               
                 t701870 
                 THCAS 
                 — 
                 0 
                 0 
                 8608.03 
                 1979.341 
                 0 
                 0 
                 0.00 
                 100.00 
                 0.00 
               
               
                   
                 Positive control 
               
               
                 t807205 
                 Library 
                 104 
                 2190.95 
                 195.13 
                 28.98 
                 57.97 
                 0.00 
                 0.00 
                 98.69 
                 1.31 
                 0.00 
               
               
                 t807272 
                 Library 
                 105 
                 28089.30 
                 1594.65 
                 1372.35 
                 166.84 
                 222.98 
                 12.56 
                 94.63 
                 4.62 
                 0.75 
               
               
                 t807301 
                 Library 
                 106 
                 16894.33 
                 3008.12 
                 934.75 
                 231.20 
                 19.38 
                 38.75 
                 94.65 
                 5.24 
                 0.11 
               
               
                 t807677 
                 Library 
                 107 
                 0.00 
                 0.00 
                 4464.43 
                 5549.24 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
               
               
                 t807764 
                 Library 
                 108 
                 8745.39 
                 2597.12 
                 1145.59 
                 313.94 
                 41.75 
                 59.04 
                 88.05 
                 11.53 
                 0.42 
               
               
                 t807774 
                 Library 
                 109 
                 23257.40 
                 2358.46 
                 1638.75 
                 138.49 
                 239.69 
                 165.44 
                 92.53 
                 6.52 
                 0.95 
               
               
                 t807810 
                 Library 
                 110 
                 12633.04 
                 5930.64 
                 547.64 
                 263.95 
                 0.00 
                 0.00 
                 95.85 
                 4.15 
                 0.00 
               
               
                 t807822 
                 Library 
                 111 
                 17911.95 
                 12548.56 
                 548.59 
                 402.89 
                 52.68 
                 105.37 
                 96.75 
                 2.96 
                 0.28 
               
               
                 t807854 
                 Library 
                 112 
                 28295.73 
                 2137.45 
                 389.02 
                 29.38 
                 309.68 
                 99.97 
                 97.59 
                 1.34 
                 1.07 
               
               
                 t807859 
                 Library 
                 113 
                 979.04 
                 1622.16 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
                 0.00 
               
               
                 t807860 
                 Library 
                 114 
                 6059.67 
                 9428.46 
                 242.75 
                 379.93 
                 88.08 
                 136.55 
                 94.82 
                 3.80 
                 1.38 
               
               
                 t807861 
                 Library 
                 115 
                 1263.83 
                 1366.48 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
                 0.00 
               
               
                 t807863 
                 Library 
                 116 
                 2009.31 
                 2653.08 
                 17.48 
                 49.43 
                 0.00 
                 0.00 
                 99.14 
                 0.86 
                 0.00 
               
               
                 t807866 
                 Library 
                 117 
                 4331.01 
                 6721.32 
                 137.76 
                 213.75 
                 14.26 
                 34.94 
                 96.61 
                 3.07 
                 0.32 
               
               
                 t807869 
                 Library 
                 118 
                 7944.59 
                 10155.04 
                 281.60 
                 464.93 
                 0.00 
                 0.00 
                 96.58 
                 3.42 
                 0.00 
               
               
                 t807873 
                 Library 
                 120 
                 18433.59 
                 705.23 
                 1175.62 
                 144.22 
                 85.27 
                 135.97 
                 93.60 
                 5.97 
                 0.43 
               
               
                 t807878 
                 Library 
                 121 
                 8442.32 
                 9157.09 
                 315.30 
                 360.65 
                 110.64 
                 136.38 
                 95.20 
                 3.56 
                 1.25 
               
               
                 t807881 
                 Library 
                 122 
                 5077.61 
                 7218.42 
                 192.96 
                 320.81 
                 44.99 
                 84.48 
                 95.52 
                 3.63 
                 0.85 
               
               
                 t807883 
                 Library 
                 123 
                 4606.20 
                 7284.45 
                 181.54 
                 281.46 
                 0.00 
                 0.00 
                 96.21 
                 3.79 
                 0.00 
               
               
                 t807917 
                 Library 
                 124 
                 12476.94 
                 3431.70 
                 600.43 
                 166.06 
                 0.00 
                 0.00 
                 95.41 
                 4.59 
                 0.00 
               
               
                 t807918 
                 Library 
                 125 
                 16735.84 
                 2219.45 
                 1065.19 
                 112.89 
                 119.68 
                 87.28 
                 93.39 
                 5.94 
                 0.67 
               
               
                 t807926 
                 Library 
                 126 
                 26139.45 
                 4019.03 
                 1101.73 
                 185.88 
                 18.67 
                 37.34 
                 95.89 
                 4.04 
                 0.07 
               
               
                 t807928 
                 Library 
                 127 
                 22647.99 
                 1997.52 
                 1240.90 
                 218.30 
                 136.60 
                 95.46 
                 94.27 
                 5.16 
                 0.57 
               
               
                 t807929 
                 Library 
                 128 
                 4498.23 
                 4252.58 
                 119.42 
                 238.83 
                 0.00 
                 0.00 
                 97.41 
                 2.59 
                 0.00 
               
               
                 t807930 
                 Library 
                 129 
                 23580.19 
                 2507.70 
                 1014.24 
                 166.36 
                 0.00 
                 0.00 
                 95.88 
                 4.12 
                 0.00 
               
               
                 t807933 
                 Library 
                 130 
                 26844.72 
                 4730.41 
                 1040.73 
                 129.25 
                 178.27 
                 23.02 
                 95.66 
                 3.71 
                 0.64 
               
               
                 t807943 
                 Library 
                 131 
                 14764.41 
                 5042.77 
                 781.93 
                 369.01 
                 27.46 
                 54.92 
                 94.80 
                 5.02 
                 0.18 
               
               
                 t807945 
                 Library 
                 132 
                 333.08 
                 385.97 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
                 0.00 
               
               
                 t807950 
                 Library 
                 134 
                 28235.47 
                 5978.18 
                 1351.19 
                 306.97 
                 46.31 
                 57.36 
                 95.28 
                 4.56 
                 0.16 
               
               
                 t807955 
                 Library 
                 135 
                 18487.09 
                 3459.56 
                 1410.52 
                 211.16 
                 195.38 
                 228.38 
                 92.01 
                 7.02 
                 0.97 
               
               
                 t807965 
                 Library 
                 136 
                 20155.49 
                 3425.87 
                 1240.06 
                 94.02 
                 227.49 
                 51.37 
                 93.21 
                 5.73 
                 1.05 
               
               
                 t807974 
                 Library 
                 137 
                 0.00 
                 0.00 
                 136.24 
                 191.02 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
               
               
                 t807980 
                 Library 
                 138 
                 17555.95 
                 10045.15 
                 806.09 
                 358.39 
                 0.00 
                 0.00 
                 95.61 
                 4.39 
                 0.00 
               
               
                 t808013 
                 Library 
                 139 
                 12365.50 
                 1671.57 
                 568.09 
                 55.87 
                 0.00 
                 0.00 
                 95.61 
                 4.39 
                 0.00 
               
               
                 t808014 
                 Library 
                 140 
                 20225.49 
                 3555.31 
                 1665.44 
                 419.41 
                 327.63 
                 58.07 
                 91.03 
                 7.50 
                 1.47 
               
               
                 t808021 
                 Library 
                 141 
                 27854.09 
                 2394.77 
                 1180.40 
                 174.07 
                 0.00 
                 0.00 
                 95.93 
                 4.07 
                 0.00 
               
               
                 t808022 
                 Library 
                 142 
                 26546.08 
                 3396.30 
                 1197.03 
                 149.25 
                 33.24 
                 66.47 
                 95.57 
                 4.31 
                 0.12 
               
               
                 t808024 
                 Library 
                 143 
                 23438.63 
                 5403.63 
                 1364.49 
                 198.52 
                 176.59 
                 35.94 
                 93.83 
                 5.46 
                 0.71 
               
               
                 t808026 
                 Library 
                 144 
                 26319.85 
                 4554.96 
                 1317.85 
                 203.24 
                 101.58 
                 74.46 
                 94.88 
                 4.75 
                 0.37 
               
               
                 t808029 
                 Library 
                 145 
                 17841.91 
                 6669.16 
                 781.98 
                 293.41 
                 51.99 
                 60.16 
                 95.53 
                 4.19 
                 0.28 
               
               
                 t808039 
                 Library 
                 146 
                 12361.14 
                 4562.70 
                 543.01 
                 180.84 
                 0.00 
                 0.00 
                 95.79 
                 4.21 
                 0.00 
               
               
                 t808040 
                 Library 
                 147 
                 7960.31 
                 3266.01 
                 500.18 
                 196.90 
                 0.00 
                 0.00 
                 94.09 
                 5.91 
                 0.00 
               
               
                 t808041 
                 Library 
                 148 
                 166.10 
                 332.19 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
                 0.00 
               
               
                 t808045 
                 Library 
                 149 
                 0.00 
                 0.00 
                 41807.82 
                 5921.89 
                 173.71 
                 45.77 
                 0.00 
                 99.59 
                 0.41 
               
               
                 t808046 
                 Library 
                 150 
                 28934.98 
                 3189.39 
                 1236.39 
                 16.70 
                 52.38 
                 74.08 
                 95.74 
                 4.09 
                 0.17 
               
               
                 t808051 
                 Library 
                 151 
                 19541.60 
                 3262.21 
                 1412.60 
                 204.83 
                 0.00 
                 0.00 
                 93.26 
                 6.74 
                 0.00 
               
               
                 t808061 
                 Library 
                 152 
                 18022.20 
                 2272.19 
                 975.95 
                 149.37 
                 22.10 
                 44.21 
                 94.75 
                 5.13 
                 0.12 
               
               
                 t808069 
                 Library 
                 153 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 145.45 
                 168.05 
                 0.00 
                 0.00 
                 100.00 
               
               
                 t808076 
                 Library 
                 154 
                 22840.65 
                 7649.22 
                 1062.37 
                 368.00 
                 53.90 
                 67.25 
                 95.34 
                 4.43 
                 0.22 
               
               
                 t808093 
                 Library 
                 155 
                 25568.84 
                 4250.97 
                 1228.66 
                 49.24 
                 25.19 
                 50.38 
                 95.33 
                 4.58 
                 0.09 
               
               
                 t808094 
                 Library 
                 156 
                 4205.58 
                 1662.08 
                 42.93 
                 85.87 
                 0.00 
                 0.00 
                 98.99 
                 1.01 
                 0.00 
               
               
                 t808103 
                 Library 
                 157 
                 19799.77 
                 2081.79 
                 1431.11 
                 215.69 
                 0.00 
                 0.00 
                 93.26 
                 6.74 
                 0.00 
               
               
                 t808125 
                 Library 
                 158 
                 5001.66 
                 1039.30 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
                 0.00 
               
               
                 t808154 
                 Library 
                 159 
                 27499.73 
                 2596.60 
                 1409.40 
                 108.39 
                 474.23 
                 30.33 
                 93.59 
                 4.80 
                 1.61 
               
               
                 t808155 
                 Library 
                 160 
                 8607.79 
                 1672.46 
                 173.09 
                 202.50 
                 0.00 
                 0.00 
                 98.03 
                 1.97 
                 0.00 
               
               
                 t808175 
                 Library 
                 161 
                 12706.15 
                 5621.21 
                 457.36 
                 89.70 
                 0.00 
                 0.00 
                 96.53 
                 3.47 
                 0.00 
               
               
                 t808177 
                 Library 
                 162 
                 29841.57 
                 1319.33 
                 1379.63 
                 80.89 
                 29.37 
                 58.75 
                 95.49 
                 4.41 
                 0.09 
               
               
                 t808199 
                 Library 
                 163 
                 30105.67 
                 6581.63 
                 1428.21 
                 352.46 
                 361.60 
                 265.65 
                 94.39 
                 4.48 
                 1.13 
               
               
                 t808200 
                 Library 
                 164 
                 29722.64 
                 7533.35 
                 1371.62 
                 266.68 
                 0.00 
                 0.00 
                 95.59 
                 4.41 
                 0.00 
               
               
                 t808223 
                 Library 
                 165 
                 30389.40 
                 2626.05 
                 1438.41 
                 75.78 
                 191.90 
                 45.95 
                 94.91 
                 4.49 
                 0.60 
               
               
                 t808225 
                 Library 
                 166 
                 27768.87 
                 2462.17 
                 1275.48 
                 125.71 
                 159.20 
                 184.57 
                 95.09 
                 4.37 
                 0.55 
               
               
                 t808226 
                 Library 
                 167 
                 28398.51 
                 6813.43 
                 1301.73 
                 240.36 
                 306.20 
                 87.33 
                 94.64 
                 4.34 
                 1.02 
               
               
                 t808232 
                 Library 
                 168 
                 20281.01 
                 3554.46 
                 1367.99 
                 178.39 
                 64.49 
                 128.99 
                 93.40 
                 6.30 
                 0.30 
               
               
                 t808237 
                 Library 
                 169 
                 12281.96 
                 2071.81 
                 760.03 
                 99.13 
                 37.34 
                 43.78 
                 93.90 
                 5.81 
                 0.29 
               
               
                 t808238 
                 Library 
                 170 
                 2934.86 
                 2769.58 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
                 0.00 
               
               
                 t808240 
                 Library 
                 171 
                 6248.43 
                 606.29 
                 115.70 
                 141.31 
                 0.00 
                 0.00 
                 98.18 
                 1.82 
                 0.00 
               
               
                 t808247 
                 Library 
                 172 
                 27052.63 
                 3600.04 
                 1703.93 
                 212.83 
                 420.85 
                 92.10 
                 92.72 
                 5.84 
                 1.44 
               
               
                 t808253 
                 Library 
                 173 
                 15518.14 
                 8165.19 
                 916.93 
                 522.30 
                 63.99 
                 127.98 
                 94.05 
                 5.56 
                 0.39 
               
               
                   
               
               
                 *The TS SEQ ID NOs provided in the table correspond to the complete protein sequence of each TS. In the context of the screen, two signal peptides were attached to each TS sequence. At the N-terminus, the N-terminal methionine was removed from each TS sequence, the TS sequence was linked to a signal peptide corresponding to SEQ ID NO: 16, and a methionine residue was added at the N-terminus of SEQ ID NO: 16. At the C-terminus, each TS sequence was linked to a signal peptide corresponding to SEQ ID NO: 17. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 CBCVA, THCVA, and CBDVA titers from metagenomic screening of CBCAS candidate enzymes in  S. cerevisiae   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                 Mean 
                 Std Dev. 
                 Mean 
                 Std Dev. 
                 Mean 
                 Std Dev 
                   
                   
                   
               
               
                 Strain 
                 Strain 
                 TS SEQ 
                 CBCVA 
                 CBCVA 
                 THCVA 
                 THCVA 
                 CBDVA 
                 CBDVA 
                 % 
                 % 
                 % 
               
               
                 ID 
                 Type 
                 ID NO* 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 CBCVA 
                 THCVA 
                 CBDVA 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 t807925 
                   A. niger  CBCAS 
                  27 
                 4473.59 
                 1643.45 
                 1821.60 
                 462.56 
                 13.83 
                 30.48 
                 70.91 
                 28.87 
                 0.22 
               
               
                   
                 Positive Control 
               
               
                 t616313 
                 GFP 
                 — 
                 319.32 
                 903.18 
                 230.36 
                 651.57 
                 0.00 
                 0.00 
                 58.09 
                 41.91 
                 0.00 
               
               
                   
                 Negative Control 
               
               
                 t616314 
                 CBDAS 
                 — 
                 19.93 
                 56.36 
                 44.29 
                 48.47 
                 1372.37 
                 356.10 
                 1.39 
                 3.08 
                 95.53 
               
               
                   
                 Positive Control 
               
               
                 t701870 
                 THCAS 
                 — 
                 280.12 
                 32.10 
                 9075.03 
                 1061.25 
                 0.00 
                 0.00 
                 2.99 
                 97.01 
                 0.00 
               
               
                   
                 Positive control 
               
               
                 t807205 
                 Library 
                 104 
                 3242.11 
                 1268.65 
                 1239.06 
                 1024.00 
                 12.91 
                 25.81 
                 72.14 
                 27.57 
                 0.29 
               
               
                 t807272 
                 Library 
                 105 
                 4874.28 
                 1877.01 
                 1842.27 
                 625.63 
                 31.94 
                 37.06 
                 72.23 
                 27.30 
                 0.47 
               
               
                 t807301 
                 Library 
                 106 
                 3187.10 
                 614.37 
                 1281.65 
                 355.07 
                 0.00 
                 0.00 
                 71.32 
                 28.68 
                 0.00 
               
               
                 t807677 
                 Library 
                 107 
                 486.77 
                 1114.57 
                 3478.94 
                 3901.07 
                 0.00 
                 0.00 
                 12.27 
                 87.73 
                 0.00 
               
               
                 t807764 
                 Library 
                 108 
                 4282.10 
                 2666.67 
                 1667.68 
                 520.12 
                 33.43 
                 47.28 
                 71.57 
                 27.87 
                 0.56 
               
               
                 t807774 
                 Library 
                 109 
                 2245.66 
                 252.04 
                 1637.38 
                 209.36 
                 0.00 
                 0.00 
                 57.83 
                 42.17 
                 0.00 
               
               
                 t807810 
                 Library 
                 110 
                 860.41 
                 278.31 
                 234.33 
                 89.52 
                 0.00 
                 0.00 
                 78.59 
                 21.41 
                 0.00 
               
               
                 t807822 
                 Library 
                 111 
                 1114.76 
                 1317.16 
                 678.58 
                 795.21 
                 0.00 
                 0.00 
                 62.16 
                 37.84 
                 0.00 
               
               
                 t807854 
                 Library 
                 112 
                 3821.61 
                 376.51 
                 820.39 
                 99.73 
                 0.00 
                 0.00 
                 82.33 
                 17.67 
                 0.00 
               
               
                 t807859 
                 Library 
                 113 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t807860 
                 Library 
                 114 
                 1489.71 
                 2036.35 
                 925.30 
                 1268.97 
                 15.49 
                 37.94 
                 61.29 
                 38.07 
                 0.64 
               
               
                 t807861 
                 Library 
                 115 
                 592.76 
                 701.24 
                 322.17 
                 406.61 
                 0.00 
                 0.00 
                 64.79 
                 35.21 
                 0.00 
               
               
                 t807863 
                 Library 
                 116 
                 979.57 
                 1212.94 
                 366.25 
                 470.73 
                 0.00 
                 0.00 
                 72.79 
                 27.21 
                 0.00 
               
               
                 t807866 
                 Library 
                 117 
                 947.47 
                 1473.69 
                 541.36 
                 838.68 
                 0.00 
                 0.00 
                 63.64 
                 36.36 
                 0.00 
               
               
                 t807869 
                 Library 
                 118 
                 1969.26 
                 1731.74 
                 1700.80 
                 1849.65 
                 12.71 
                 23.54 
                 53.47 
                 46.18 
                 0.35 
               
               
                 t807873 
                 Library 
                 120 
                 2573.41 
                 469.37 
                 1852.74 
                 248.66 
                 11.40 
                 27.92 
                 57.99 
                 41.75 
                 0.26 
               
               
                 t807878 
                 Library 
                 121 
                 1509.38 
                 1309.86 
                 1003.24 
                 903.57 
                 7.68 
                 21.71 
                 59.89 
                 39.81 
                 0.30 
               
               
                 t807881 
                 Library 
                 122 
                 1683.77 
                 1656.75 
                 754.52 
                 884.88 
                 7.88 
                 22.28 
                 68.83 
                 30.84 
                 0.32 
               
               
                 t807883 
                 Library 
                 123 
                 1858.75 
                 3607.91 
                 687.66 
                 1246.57 
                 17.57 
                 43.04 
                 72.49 
                 26.82 
                 0.69 
               
               
                 t807917 
                 Library 
                 124 
                 1836.22 
                 655.01 
                 703.90 
                 291.09 
                 0.00 
                 0.00 
                 72.29 
                 27.71 
                 0.00 
               
               
                 t807918 
                 Library 
                 125 
                 2162.77 
                 205.77 
                 1837.88 
                 182.53 
                 27.72 
                 32.03 
                 53.69 
                 45.62 
                 0.69 
               
               
                 t807926 
                 Library 
                 126 
                 2784.98 
                 913.27 
                 1285.08 
                 336.14 
                 0.00 
                 0.00 
                 68.43 
                 31.57 
                 0.00 
               
               
                 t807928 
                 Library 
                 127 
                 2566.28 
                 344.04 
                 1132.43 
                 91.93 
                 0.00 
                 0.00 
                 69.38 
                 30.62 
                 0.00 
               
               
                 t807929 
                 Library 
                 128 
                 2333.33 
                 581.53 
                 299.01 
                 71.94 
                 0.00 
                 0.00 
                 88.64 
                 11.36 
                 0.00 
               
               
                 t807930 
                 Library 
                 129 
                 2442.49 
                 556.63 
                 1212.04 
                 246.82 
                 0.00 
                 0.00 
                 66.83 
                 33.17 
                 0.00 
               
               
                 t807933 
                 Library 
                 130 
                 2408.56 
                 692.63 
                 1248.45 
                 316.40 
                 0.00 
                 0.00 
                 65.86 
                 34.14 
                 0.00 
               
               
                 t807943 
                 Library 
                 131 
                 1986.17 
                 677.42 
                 756.16 
                 148.38 
                 0.00 
                 0.00 
                 72.43 
                 27.57 
                 0.00 
               
               
                 t807945 
                 Library 
                 132 
                 161.04 
                 188.87 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
                 0.00 
               
               
                 t807950 
                 Library 
                 134 
                 3453.68 
                 613.98 
                 1656.86 
                 240.06 
                 0.00 
                 0.00 
                 67.58 
                 32.42 
                 0.00 
               
               
                 t807955 
                 Library 
                 135 
                 1978.92 
                 414.31 
                 1415.78 
                 302.91 
                 0.00 
                 0.00 
                 58.29 
                 41.71 
                 0.00 
               
               
                 t807965 
                 Library 
                 136 
                 2452.35 
                 535.40 
                 1538.67 
                 349.32 
                 0.00 
                 0.00 
                 61.45 
                 38.55 
                 0.00 
               
               
                 t807974 
                 Library 
                 137 
                 165.89 
                 331.78 
                 29.12 
                 58.23 
                 0.00 
                 0.00 
                 85.07 
                 14.93 
                 0.00 
               
               
                 t807980 
                 Library 
                 138 
                 3355.90 
                 1222.41 
                 554.16 
                 669.40 
                 35.61 
                 45.18 
                 85.05 
                 14.04 
                 0.90 
               
               
                 t808013 
                 Library 
                 139 
                 1907.14 
                 594.72 
                 789.25 
                 209.72 
                 0.00 
                 0.00 
                 70.73 
                 29.27 
                 0.00 
               
               
                 t808014 
                 Library 
                 140 
                 1762.19 
                 360.60 
                 1617.54 
                 288.58 
                 0.00 
                 0.00 
                 52.14 
                 47.86 
                 0.00 
               
               
                 t808021 
                 Library 
                 141 
                 4204.55 
                 218.50 
                 1774.95 
                 79.34 
                 0.00 
                 0.00 
                 70.32 
                 29.68 
                 0.00 
               
               
                 t808022 
                 Library 
                 142 
                 4422.08 
                 738.43 
                 1809.05 
                 196.16 
                 0.00 
                 0.00 
                 70.97 
                 29.03 
                 0.00 
               
               
                 t808024 
                 Library 
                 143 
                 2908.72 
                 808.06 
                 1276.65 
                 416.04 
                 20.03 
                 40.05 
                 69.17 
                 30.36 
                 0.48 
               
               
                 t808026 
                 Library 
                 144 
                 3270.32 
                 422.75 
                 1713.13 
                 176.34 
                 18.76 
                 37.52 
                 65.38 
                 34.25 
                 0.38 
               
               
                 t808029 
                 Library 
                 145 
                 2406.20 
                 1183.56 
                 953.65 
                 712.38 
                 18.18 
                 36.36 
                 71.23 
                 28.23 
                 0.54 
               
               
                 t808039 
                 Library 
                 146 
                 2104.54 
                 404.51 
                 747.24 
                 150.97 
                 0.00 
                 0.00 
                 73.80 
                 26.20 
                 0.00 
               
               
                 t808040 
                 Library 
                 147 
                 2925.68 
                 1239.54 
                 938.63 
                 809.00 
                 14.93 
                 29.87 
                 75.42 
                 24.20 
                 0.38 
               
               
                 t808041 
                 Library 
                 148 
                 152.65 
                 111.77 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
                 0.00 
               
               
                 t808045 
                 Library 
                 149 
                 0.00 
                 0.00 
                 9402.99 
                 1132.41 
                 0.00 
                 0.00 
                 0.00 
                 100.00 
                 0.00 
               
               
                 t808046 
                 Library 
                 150 
                 3174.69 
                 772.59 
                 1514.30 
                 295.18 
                 0.00 
                 0.00 
                 67.71 
                 32.29 
                 0.00 
               
               
                 t808051 
                 Library 
                 151 
                 2863.45 
                 1434.93 
                 2043.57 
                 770.01 
                 33.01 
                 38.44 
                 57.96 
                 41.37 
                 0.67 
               
               
                 t808061 
                 Library 
                 152 
                 2367.22 
                 114.94 
                 1495.44 
                 77.78 
                 0.00 
                 0.00 
                 61.28 
                 38.72 
                 0.00 
               
               
                 t808069 
                 Library 
                 153 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
                 169.41 
                 210.59 
                 0.00 
                 0.00 
                 100.00 
               
               
                 t808076 
                 Library 
                 154 
                 3558.84 
                 124.32 
                 1458.01 
                 189.04 
                 0.00 
                 0.00 
                 70.94 
                 29.06 
                 0.00 
               
               
                 t808093 
                 Library 
                 155 
                 3833.15 
                 875.00 
                 1280.76 
                 906.89 
                 35.24 
                 41.80 
                 74.44 
                 24.87 
                 0.68 
               
               
                 t808094 
                 Library 
                 156 
                 2498.54 
                 925.99 
                 808.41 
                 353.22 
                 0.00 
                 0.00 
                 75.55 
                 24.45 
                 0.00 
               
               
                 t808103 
                 Library 
                 157 
                 2911.66 
                 912.45 
                 2038.06 
                 496.29 
                 25.07 
                 50.15 
                 58.53 
                 40.97 
                 0.50 
               
               
                 t808125 
                 Library 
                 158 
                 3288.83 
                 840.09 
                 595.19 
                 150.14 
                 0.00 
                 0.00 
                 84.68 
                 15.32 
                 0.00 
               
               
                 t808154 
                 Library 
                 159 
                 3740.08 
                 532.10 
                 1882.39 
                 217.34 
                 0.00 
                 0.00 
                 66.52 
                 33.48 
                 0.00 
               
               
                 t808155 
                 Library 
                 160 
                 4173.38 
                 1767.24 
                 1063.02 
                 315.81 
                 0.00 
                 0.00 
                 79.70 
                 20.30 
                 0.00 
               
               
                 t808175 
                 Library 
                 161 
                 1838.07 
                 137.92 
                 635.41 
                 516.48 
                 8.73 
                 17.47 
                 74.05 
                 25.60 
                 0.35 
               
               
                 t808177 
                 Library 
                 162 
                 3018.88 
                 539.22 
                 1053.71 
                 728.24 
                 17.94 
                 35.88 
                 73.80 
                 25.76 
                 0.44 
               
               
                 t808199 
                 Library 
                 163 
                 3733.69 
                 2406.71 
                 1651.60 
                 1693.22 
                 25.91 
                 51.83 
                 69.00 
                 30.52 
                 0.48 
               
               
                 t808200 
                 Library 
                 164 
                 3073.87 
                 538.39 
                 1507.03 
                 239.98 
                 0.00 
                 0.00 
                 67.10 
                 32.90 
                 0.00 
               
               
                 t808223 
                 Library 
                 165 
                 3592.30 
                 439.40 
                 1636.00 
                 155.56 
                 0.00 
                 0.00 
                 68.71 
                 31.29 
                 0.00 
               
               
                 t808225 
                 Library 
                 166 
                 3608.44 
                 825.78 
                 1476.48 
                 1038.39 
                 27.78 
                 55.57 
                 70.58 
                 28.88 
                 0.54 
               
               
                 t808226 
                 Library 
                 167 
                 4553.40 
                 2121.13 
                 2421.15 
                 654.66 
                 64.86 
                 48.17 
                 64.68 
                 34.39 
                 0.92 
               
               
                 t808232 
                 Library 
                 168 
                 2379.23 
                 352.45 
                 1626.74 
                 243.69 
                 0.00 
                 0.00 
                 59.39 
                 40.61 
                 0.00 
               
               
                 t808237 
                 Library 
                 169 
                 3599.07 
                 1154.82 
                 1273.10 
                 423.30 
                 0.00 
                 0.00 
                 73.87 
                 26.13 
                 0.00 
               
               
                 t808238 
                 Library 
                 170 
                 1841.90 
                 684.06 
                 282.10 
                 414.39 
                 0.00 
                 0.00 
                 86.72 
                 13.28 
                 0.00 
               
               
                 t808240 
                 Library 
                 171 
                 4282.13 
                 1030.26 
                 888.75 
                 394.89 
                 0.00 
                 0.00 
                 82.81 
                 17.19 
                 0.00 
               
               
                 t808247 
                 Library 
                 172 
                 2651.55 
                 513.12 
                 1783.19 
                 177.55 
                 15.16 
                 30.31 
                 59.59 
                 40.07 
                 0.34 
               
               
                 t808253 
                 Library 
                 173 
                 1476.99 
                 735.60 
                 715.85 
                 720.66 
                 0.00 
                 0.00 
                 67.35 
                 32.65 
                 0.00 
               
               
                   
               
               
                 *The TS SEQ ID NOs provided in the table correspond to the complete protein sequence of each TS. In the context of the screen, two signal peptides were attached to each TS sequence. At the N-terminus, the N-terminal methionine was removed from each TS sequence, the TS sequence was linked to a signal peptide corresponding to SEQ ID NO: 16, and a methionine residue was added at the N-terminus of SEQ ID NO: 16. At the C-terminus, each TS sequence was linked to a signal peptide corresponding to SEQ ID NO: 17. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 CBGA and CBGVA residual substrate from metagenomic screening 
               
               
                 of CBCAS candidate enzymes in  S. cerevisiae   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Standard 
                   
                 Standard 
               
               
                   
                   
                   
                 Average 
                 Deviation 
                 Average 
                 Deviation 
               
               
                 Strain 
                   
                 TS SEQ 
                 CBGA 
                 CBGA 
                 CBGVA 
                 CBGVA 
               
               
                 ID 
                 Strain Type 
                 ID NO* 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
                 [μg/L] 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 t807925 
                   A. niger  CBCAS 
                  27 
                 19.90 
                 45.80 
                 0.00 
                 0.00 
               
               
                   
                 Positive Control 
               
               
                 t616313 
                 GFP 
                 — 
                 59298.53 
                 5174.35 
                 21898.05 
                 10583.34 
               
               
                   
                 Negative Control 
               
               
                 t807205 
                 Library 
                 104 
                 53147.96 
                 12834.43 
                 3437.64 
                 2892.55 
               
               
                 t807272 
                 Library 
                 105 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t807301 
                 Library 
                 106 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t807677 
                 Library 
                 107 
                 52271.45 
                 7668.39 
                 11977.90 
                 8565.71 
               
               
                 t807764 
                 Library 
                 108 
                 40451.56 
                 9639.86 
                 311.78 
                 236.61 
               
               
                 t807774 
                 Library 
                 109 
                 32.82 
                 65.65 
                 0.00 
                 0.00 
               
               
                 t807810 
                 Library 
                 110 
                 380.38 
                 703.07 
                 0.00 
                 0.00 
               
               
                 t807822 
                 Library 
                 111 
                 538.72 
                 1077.45 
                 16.99 
                 33.97 
               
               
                 t807854 
                 Library 
                 112 
                 8963.64 
                 3478.68 
                 0.00 
                 0.00 
               
               
                 t807859 
                 Library 
                 113 
                 63345.00 
                 14967.80 
                 17522.15 
                 3427.61 
               
               
                 t807860 
                 Library 
                 114 
                 43908.19 
                 31951.06 
                 9772.66 
                 11054.13 
               
               
                 t807861 
                 Library 
                 115 
                 62687.37 
                 12260.30 
                 16647.73 
                 4876.37 
               
               
                 t807863 
                 Library 
                 116 
                 48851.59 
                 9711.58 
                 16336.42 
                 8135.29 
               
               
                 t807866 
                 Library 
                 117 
                 36035.77 
                 11249.90 
                 10751.97 
                 9127.95 
               
               
                 t807869 
                 Library 
                 118 
                 42005.98 
                 26148.08 
                 7246.67 
                 9148.58 
               
               
                 t807873 
                 Library 
                 120 
                 20.28 
                 49.68 
                 0.00 
                 0.00 
               
               
                 t807878 
                 Library 
                 121 
                 38442.99 
                 20155.33 
                 5151.50 
                 7882.93 
               
               
                 t807881 
                 Library 
                 122 
                 46732.64 
                 18976.53 
                 11406.58 
                 10063.07 
               
               
                 t807883 
                 Library 
                 123 
                 42814.16 
                 9130.34 
                 12651.49 
                 10100.78 
               
               
                 t807917 
                 Library 
                 124 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t807918 
                 Library 
                 125 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t807926 
                 Library 
                 126 
                 57.58 
                 67.71 
                 0.00 
                 0.00 
               
               
                 t807928 
                 Library 
                 127 
                 25.47 
                 50.94 
                 0.00 
                 0.00 
               
               
                 t807929 
                 Library 
                 128 
                 41396.36 
                 27087.65 
                 15214.71 
                 1846.68 
               
               
                 t807930 
                 Library 
                 129 
                 44.74 
                 89.48 
                 0.00 
                 0.00 
               
               
                 t807933 
                 Library 
                 130 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t807943 
                 Library 
                 131 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t807945 
                 Library 
                 132 
                 55188.82 
                 15675.50 
                 22716.84 
                 10015.46 
               
               
                 t807950 
                 Library 
                 134 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t807955 
                 Library 
                 135 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t807965 
                 Library 
                 136 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t807974 
                 Library 
                 137 
                 48233.77 
                 33615.86 
                 20337.45 
                 1273.42 
               
               
                 t807980 
                 Library 
                 138 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808013 
                 Library 
                 139 
                 35.97 
                 71.94 
                 0.00 
                 0.00 
               
               
                 t808014 
                 Library 
                 140 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808021 
                 Library 
                 141 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808022 
                 Library 
                 142 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808024 
                 Library 
                 143 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808026 
                 Library 
                 144 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808029 
                 Library 
                 145 
                 39.53 
                 79.06 
                 0.00 
                 0.00 
               
               
                 t808039 
                 Library 
                 146 
                 53.06 
                 106.12 
                 0.00 
                 0.00 
               
               
                 t808040 
                 Library 
                 147 
                 10397.55 
                 7554.81 
                 60.04 
                 72.45 
               
               
                 t808041 
                 Library 
                 148 
                 43557.01 
                 9983.47 
                 30246.69 
                 9758.25 
               
               
                 t808045 
                 Library 
                 149 
                 575.78 
                 450.99 
                 0.00 
                 0.00 
               
               
                 t808046 
                 Library 
                 150 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808051 
                 Library 
                 151 
                 28.31 
                 56.61 
                 0.00 
                 0.00 
               
               
                 t808061 
                 Library 
                 152 
                 34.71 
                 69.42 
                 0.00 
                 0.00 
               
               
                 t808069 
                 Library 
                 153 
                 53474.30 
                 8943.22 
                 13875.42 
                 911.61 
               
               
                 t808076 
                 Library 
                 154 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808093 
                 Library 
                 155 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808094 
                 Library 
                 156 
                 31781.07 
                 13527.80 
                 2741.81 
                 2696.82 
               
               
                 t808103 
                 Library 
                 157 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808125 
                 Library 
                 158 
                 53834.41 
                 9317.13 
                 3639.01 
                 1236.20 
               
               
                 t808154 
                 Library 
                 159 
                 1056.05 
                 420.68 
                 0.00 
                 0.00 
               
               
                 t808155 
                 Library 
                 160 
                 21117.02 
                 9763.61 
                 23.86 
                 47.72 
               
               
                 t808175 
                 Library 
                 161 
                 8034.51 
                 16069.03 
                 0.00 
                 0.00 
               
               
                 t808177 
                 Library 
                 162 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808199 
                 Library 
                 163 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808200 
                 Library 
                 164 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808223 
                 Library 
                 165 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808225 
                 Library 
                 166 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808226 
                 Library 
                 167 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808232 
                 Library 
                 168 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808237 
                 Library 
                 169 
                 69.20 
                 138.40 
                 0.00 
                 0.00 
               
               
                 t808238 
                 Library 
                 170 
                 63815.30 
                 9562.86 
                 9247.47 
                 6162.29 
               
               
                 t808240 
                 Library 
                 171 
                 24393.82 
                 2396.56 
                 4054.85 
                 4444.75 
               
               
                 t808247 
                 Library 
                 172 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                 t808253 
                 Library 
                 173 
                 0.00 
                 0.00 
                 0.00 
                 0.00 
               
               
                   
               
               
                 *The TS SEQ ID NOs provided in the table correspond to the complete protein sequence of each TS. In the context of the screen, two signal peptides were attached to each TS sequence. At the N-terminus, the N-terminal methionine was removed from each TS sequence, the TS sequence was linked to a signal peptide corresponding to SEQ ID NO: 16, and a methionine residue was added at the N-terminus of SEQ ID NO: 16. At the C-terminus, each TS sequence was linked to a signal peptide corresponding to SEQ ID NO: 17. 
               
            
           
         
       
     
     Example 4: Assessment of the Requirement for Signal Peptides for CBCAS Activity 
     Post-translational modifications (e.g., the formation of intramolecular disulfide bridges, post-translational glycosylation, etc.) are known to be important for the activity of  Cannabis  terminal synthases. The presence of signal peptides on terminal synthase enzymes may help facilitate the post-translational modifications. However, it was unknown whether the  A. niger  CBCAS identified in Example 1, or the additional CBCASs identified in Example 3, required signal peptides to be active. 
     A library of 20 CBCAS enzymes selected from Example 1 and 3 was synthesized, including versions of the CBCAS enzymes with and without the N-terminal MFalpha2 signal peptide (SEQ ID NO: 16) and C-terminal HDEL signal peptide (SEQ ID NO: 17). Each candidate enzyme expression construct was transformed into an  S. cerevisiae  CEN.PK strain that also expressed a prenyltransferase enzyme capable of catalyzing reaction R 4  in  FIG.  2   . Strain t861555 expressing the  A. niger  CBCAS identified in Example 1, carrying both the Mfalpha2 and HDEL signal peptides was included in the library screen as a positive control for enzyme activity. Strain t861565 expressed the same  A. niger  CBCAS without the Mfalpha2 and HDEL signal peptides. 
     The strains were screened using the assay described in Example 1 with the following exception: at Day 4 samples were not subjected to a pH adjustment and a further 2 days of incubation at 20° C. 
     12 strains demonstrated greater mean CBCAS activity than that of the t861555 positive control ( FIG.  14   , Table 13). Surprisingly, the impact of the two signal peptides was found to vary depending on the identity of the CBCAS candidate: in some instances, the presence of both signal peptides was observed to enhance CBCAS activity, while in other instances, it was observed to reduce activity. The absence of the two signal peptides from the  A. niger  CBCAS had a significant positive impact on CBCAS activity. The t861565 strain, expressing the  A. niger  CBCAS without signal peptides demonstrated approximately 4-fold higher CBCA titer than the t861555 strain, expressing the  A. niger  CBCAS with signal peptides. 
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 CBCA titers from screening of CBCAS candidate enzymes 
               
               
                 with and without signal peptides in  S. cerevisiae   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                 N-terminal and 
                   
                   
               
               
                   
                   
                   
                 C-terminal 
                   
                 Standard 
               
               
                   
                   
                   
                 peptides 
                 Average 
                 Deviation 
               
               
                 Strain 
                   
                 TS SEQ 
                 [Y = Yes 
                 CBCA 
                 CBCA 
               
               
                 ID 
                 Strain Type 
                 ID NO* 
                 N = No] 
                 [μg/L] 
                 [μg/L] 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 t861555 
                   A. niger  CBCAS 
                 27 
                 Y 
                 21237.64 
                 22960.70 
               
               
                   
                 Pos. Ctrl. 
               
               
                 t861565 
                   A. niger  CBCAS 
                 27 
                 N 
                 78892.80 
                 10755.89 
               
               
                   
                 Pos. Ctrl. 
               
               
                 t861557 
                 Library 
                 144 
                 Y 
                 520.64 
                 901.77 
               
               
                 t861584 
                 Library 
                 144 
                 N 
                 0.00 
                 0.00 
               
               
                 t861559 
                 Library 
                 150 
                 Y 
                 0.00 
                 0.00 
               
               
                 t861586 
                 Library 
                 150 
                 N 
                 0.00 
                 0.00 
               
               
                 t861591 
                 Library 
                 141 
                 Y 
                 0.00 
                 0.00 
               
               
                 t861573 
                 Library 
                 141 
                 N 
                 0.00 
                 0.00 
               
               
                 t861562 
                 Library 
                 167 
                 Y 
                 55737.91 
                 20610.57 
               
               
                 t861582 
                 Library 
                 167 
                 N 
                 20912.35 
                 6804.79 
               
               
                 t861563 
                 Library 
                 112 
                 Y 
                 4821.60 
                 3851.63 
               
               
                 t861553 
                 Library 
                 112 
                 N 
                 2393.08 
                 2024.49 
               
               
                 t861551 
                 Library 
                 105 
                 Y 
                 17501.94 
                 8781.47 
               
               
                 t861578 
                 Library 
                 105 
                 N 
                 62171.35 
                 31734.93 
               
               
                 t861568 
                 Library 
                 142 
                 Y 
                 0.00 
                 0.00 
               
               
                 t861576 
                 Library 
                 142 
                 N 
                 0.00 
                 0.00 
               
               
                 t861588 
                 Library 
                 163 
                 Y 
                 42686.95 
                 11722.91 
               
               
                 t861564 
                 Library 
                 163 
                 N 
                 12924.20 
                 3312.59 
               
               
                 t861567 
                 Library 
                 154 
                 Y 
                 0.00 
                 0.00 
               
               
                 t861575 
                 Library 
                 154 
                 N 
                 0.00 
                 0.00 
               
               
                 t861577 
                 Library 
                 126 
                 Y 
                 36869.19 
                 8966.99 
               
               
                 t861592 
                 Library 
                 126 
                 N 
                 74584.36 
                 5016.15 
               
               
                 t861583 
                 Library 
                 162 
                 Y 
                 59260.52 
                 5672.49 
               
               
                 t861589 
                 Library 
                 162 
                 N 
                 95796.21 
                 18887.68 
               
               
                 t861566 
                 Library 
                 155 
                 Y 
                 61918.09 
                 9713.74 
               
               
                 t861587 
                 Library 
                 155 
                 N 
                 82883.01 
                 5160.26 
               
               
                 t861554 
                 Library 
                 159 
                 Y 
                 5334.71 
                 N/A** 
               
               
                 t861552 
                 Library 
                 159 
                 N 
                 15253.62 
                 3086.10 
               
               
                 t861574 
                 Library 
                 164 
                 Y 
                 38142.03 
                 31232.36 
               
               
                 t861572 
                 Library 
                 164 
                 N 
                 61793.56 
                 7141.71 
               
               
                 t861558 
                 Library 
                 134 
                 Y 
                 27898.00 
                 15692.88 
               
               
                 t861590 
                 Library 
                 134 
                 N 
                 55852.93 
                 43778.21 
               
               
                 t861580 
                 Library 
                 143 
                 Y 
                 0.00 
                 0.00 
               
               
                 t861570 
                 Library 
                 143 
                 N 
                 0.00 
                 0.00 
               
               
                 t861579 
                 Library 
                 172 
                 Y 
                 57912.84 
                 5105.04 
               
               
                 t861556 
                 Library 
                 172 
                 N 
                 50870.36 
                 1457.77 
               
               
                 t861571 
                 Library 
                 165 
                 Y 
                 54271.76 
                 2447.30 
               
               
                 t861569 
                 Library 
                 165 
                 N 
                 36631.83 
                 6800.49 
               
               
                 t861561 
                 Library 
                 166 
                 Y 
                 46161.25 
                 5238.08 
               
               
                 t861560 
                 Library 
                 166 
                 N 
                 16325.34 
                 14173.22 
               
               
                 t861585 
                 Library 
                 130 
                 Y 
                 39673.45 
                 15792.21 
               
               
                 t861581 
                 Library 
                 130 
                 N 
                 38663.23 
                 6553.85 
               
               
                   
               
               
                 *The TS SEQ ID NOs provided in the table correspond to the complete protein sequence of each TS. In the context of the screen, for the strains that are indicated as “Y” for expressing the TS sequence with signal peptides, two signal peptides were attached to each TS sequence. At the N-terminus, the N-terminal methionine was removed from each TS sequence, the TS sequence was linked to a signal peptide corresponding to SEQ ID NO: 16, and a methionine residue was added at the N-terminus of SEQ ID NO: 16. At the C-terminus, each TS sequence was linked to a signal peptide corresponding to SEQ ID NO: 17. 
               
               
                 **single bioreplicate, standard deviation not applicable 
               
            
           
         
       
     
     Example 5: Identification of Sequence Motifs Enriched in CBCAS Enzymes Identified in Examples 1-4 
     Analysis of CBCAS enzymes from Example 4 identified multiple sequence motifs that were enriched in CBCAS enzymes that produced a mean CBCA titer greater than the  A. niger  CBCAS. Table 14 provides sequence information for the motifs identified. 
     Structural models were generated using crystal structures from related proteins to determine where the sequence motifs localize within the 3-dimensional structure of a TS enzyme.  FIGS.  15  and  16    depict ribbon diagrams showing predicted localization of several of the identified sequence motifs. Sequence motifs KVQARSGGH (SEQ ID NO: 174), CPTI[KR]TGGH (SEQ ID NO: 181), and P[IV]S[DQE]TTY[EDG]F[TA]DGLYDVLA[RQK]AVPES[VA]GHAYLGCPDP[RK]M (SEQ ID NO: 186), indicated by arrows in  FIG.  15   , are predicted to contact the cofactor binding site and may therefore influence cofactor binding. 
     The motif RT[EQ][PQ]APGLAVQYSY (SEQ ID NO: 207), indicated by an arrow in  FIG.  16   , is predicted to be near the substrate binding pocket. The motif WQ[SA]FI[SA][AQ][KE]NLT[RW][QK]FY[NST]NM (SEQ ID NO: 211), indicated by an arrow in  FIG.  16   , is predicted to line the cavity of the active site and may potentially influence substrate or product specificity. 
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Motif sequences identified in candidate CBCASs 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Reference 
                   
                   
                   
               
               
                   
                 sequence (SEQ 
                   
                   
                   
               
               
                   
                 ID NO: 27) 
                 Motif sequence 
                   
                 TS SEQ 
               
            
           
           
               
               
               
               
               
               
            
               
                 Motif 
                 start 
                 end 
                 in strain 
                 Strain* 
                 ID NO** 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 KVQARSGGH (SEQ ID NO: 174) 
                 72 
                 80 
                 KVQARSGGH 
                 t861555 
                 27 
               
               
                   
                   
                   
                 (SEQ ID NO: 
                 t861565 
                   
               
               
                   
                   
                   
                 174) 
                 t861579 
                 172 
               
               
                   
                   
                   
                   
                 t861556 
                   
               
               
                   
                   
                   
                   
                 t861561 
                 166 
               
               
                   
                   
                   
                   
                 t861560 
                   
               
               
                   
                   
                   
                   
                 t861554 
                 159 
               
               
                   
                   
                   
                   
                 t861552 
                   
               
               
                   
                   
                   
                   
                 t861588 
                 163 
               
               
                   
                   
                   
                   
                 t861564 
                   
               
               
                   
                   
                   
                   
                 t861562 
                 167 
               
               
                   
                   
                   
                   
                 t861582 
                   
               
               
                   
                   
                   
                   
                 t861571 
                 165 
               
               
                   
                   
                   
                   
                 t861569 
                   
               
               
                   
                   
                   
                   
                 t861583 
                 162 
               
               
                   
                   
                   
                   
                 t861589 
                   
               
               
                   
                   
                   
                   
                 t861558 
                 134 
               
               
                   
                   
                   
                   
                 t861590 
                   
               
               
                   
                   
                   
                   
                 t861574 
                 164 
               
               
                   
                   
                   
                   
                 t861572 
                   
               
               
                   
                   
                   
                   
                 t861551 
                 105 
               
               
                   
                   
                   
                   
                 t861578 
                   
               
               
                   
                   
                   
                   
                 t861577 
                 126 
               
               
                   
                   
                   
                   
                 t861592 
                   
               
               
                   
                   
                   
                   
                 t861566 
                 155 
               
               
                   
                   
                   
                   
                 t861587 
                   
               
               
                   
                   
                   
                   
                 t861563 
                 112 
               
               
                   
                   
                   
                   
                 t861553 
                   
               
               
                   
                   
                   
                   
                 t861585 
                 130 
               
               
                   
                   
                   
                   
                 t861581 
                   
               
               
                   
               
               
                 RASNTQNQD[VI][FL]FA[VI]K (SEQ 
                 183 
                 197 
                 RASNTQNQDVF 
                 t861555 
                 27 
               
               
                 ID NO: 176) 
                   
                   
                 FAVK (SEQ ID 
                 t861565 
                   
               
               
                   
                   
                   
                 NO: 177) 
                 t861571 
                 165 
               
               
                   
                   
                   
                   
                 t861569 
                   
               
               
                   
                   
                   
                   
                 t861583 
                 162 
               
               
                   
                   
                   
                   
                 t861589 
                   
               
               
                   
                   
                   
                   
                 t861558 
                 134 
               
               
                   
                   
                   
                   
                 t861590 
                   
               
               
                   
                   
                   
                   
                 t861574 
                 164 
               
               
                   
                   
                   
                   
                 t861572 
                   
               
               
                   
                   
                   
                   
                 t861551 
                 105 
               
               
                   
                   
                   
                   
                 t861578 
                   
               
               
                   
                   
                   
                   
                 t861566 
                 155 
               
               
                   
                   
                   
                   
                 t861587 
                   
               
               
                   
                   
                   
                 RASNTQNQDIL 
                 t861579 
                 172 
               
               
                   
                   
                   
                 FAVK (SEQ ID 
                 t861556 
                   
               
               
                   
                   
                   
                 NO: 178) 
                   
                   
               
               
                   
                   
                   
                 RASNTQNQDIL 
                 t861588 
                 163 
               
               
                   
                   
                   
                 FAIK (SEQ ID 
                 t861564 
                   
               
               
                   
                   
                   
                 NO: 179) 
                   
                   
               
               
                   
                   
                   
                 RASNTQNQDV 
                 t861577 
                 126 
               
               
                   
                   
                   
                 LFAVK (SEQ ID 
                 t861592 
                   
               
               
                   
                   
                   
                 NO: 180) 
                   
                   
               
               
                   
               
               
                 CPTI[KR]TGGH (SEQ ID NO: 181) 
                 141 
                 149 
                 CPTIKTGGH 
                 t861555 
                 27 
               
               
                   
                   
                   
                 (SEQ ID NO: 
                 t861565 
                   
               
               
                   
                   
                   
                 182) 
                 t861571 
                 165 
               
               
                   
                   
                   
                   
                 t861569 
                   
               
               
                   
                   
                   
                   
                 t861583 
                 162 
               
               
                   
                   
                   
                   
                 t861589 
                   
               
               
                   
                   
                   
                   
                 t861558 
                 134 
               
               
                   
                   
                   
                   
                 t861590 
                   
               
               
                   
                   
                   
                   
                 t861574 
                 164 
               
               
                   
                   
                   
                   
                 t861572 
                   
               
               
                   
                   
                   
                   
                 t861551 
                 105 
               
               
                   
                   
                   
                   
                 t861578 
                   
               
               
                   
                   
                   
                   
                 t861577 
                 126 
               
               
                   
                   
                   
                   
                 t861592 
                   
               
               
                   
                   
                   
                   
                 t861566 
                 155 
               
               
                   
                   
                   
                   
                 t861587 
                   
               
               
                   
                   
                   
                 CPTIRTGGH 
                 t861579 
                 172 
               
               
                   
                   
                   
                 (SEQ ID NO: 
                 t861556 
                   
               
               
                   
                   
                   
                 183) 
                 t861561 
                 166 
               
               
                   
                   
                   
                   
                 t861560 
                   
               
               
                   
                   
                   
                   
                 t861554 
                 159 
               
               
                   
                   
                   
                   
                 t861552 
                   
               
               
                   
                   
                   
                   
                 t861588 
                 163 
               
               
                   
                   
                   
                   
                 t861564 
                   
               
               
                   
                   
                   
                   
                 t861562 
                 167 
               
               
                   
                   
                   
                   
                 t861582 
                   
               
               
                   
               
               
                 WFVTLSLEGGAINDV[AP]EDATAY 
                 360 
                 383 
                 WFVTLSLEGGA 
                 t861555 
                 27 
               
               
                 [AG]H (SEQ ID NO: 184) 
                   
                   
                 INDVAEDATAY 
                 t861565 
                   
               
               
                   
                   
                   
                 AH (SEQ ID NO: 
                 t861571 
                 165 
               
               
                   
                   
                   
                 185) 
                 t861569 
                   
               
               
                   
                   
                   
                   
                 t861583 
                 162 
               
               
                   
                   
                   
                   
                 t861589 
                   
               
               
                   
                   
                   
                   
                 t861551 
                 105 
               
               
                   
                   
                   
                   
                 t861578 
                   
               
               
                   
                   
                   
                   
                 t861577 
                 126 
               
               
                   
                   
                   
                   
                 t861592 
                   
               
               
                   
                   
                   
                   
                 t861566 
                 155 
               
               
                   
                   
                   
                   
                 t861587 
                   
               
               
                   
               
               
                 P[IV]S[DQE]TTY[EDG]F[TA]DGLY 
                 400 
                 436 
                 PISDTTYEFTDG 
                 t861555 
                 27 
               
               
                 DVLA[RQK]AVPES[VA]GHAYLGC 
                   
                   
                 SVGHAYLGCPD 
                 t861565 
                   
               
               
                 PDP[RK]M (SEQ ID NO: 186) 
                   
                   
                 LYDVLARAVPE 
                 t861571 
                 165 
               
               
                   
                   
                   
                 PRM (SEQ ID 
                 t861569 
                   
               
               
                   
                   
                   
                 NO: 187) 
                 t861583 
                 162 
               
               
                   
                   
                   
                   
                 t861589 
                   
               
               
                   
                   
                   
                   
                 t861558 
                 134 
               
               
                   
                   
                   
                   
                 t861590 
                   
               
               
                   
                   
                   
                   
                 t861574 
                 164 
               
               
                   
                   
                   
                   
                 t861572 
                   
               
               
                   
                   
                   
                 PISETTYEFTDG 
                 t861551 
                 105 
               
               
                   
                   
                   
                 LYDVLARAVPE 
                 t861578 
                   
               
               
                   
                   
                   
                 SVGHAYLGCPD 
                 t861577 
                 126 
               
               
                   
                   
                   
                 PRM (SEQ ID 
                 t861592 
                   
               
               
                   
                   
                   
                 NO: 188) 
                 t861566 
                 155 
               
               
                   
                   
                   
                   
                 t861587 
                   
               
               
                   
               
               
                 MKHF[TNS]QFSM (SEQ ID NO: 189) 
                 98 
                 106 
                 MKHFTQFSM 
                 t861555 
                 27 
               
               
                   
                   
                   
                 (SEQ ID NO: 
                 t861565 
                   
               
               
                   
                   
                   
                 190) 
                 t861571 
                 165 
               
               
                   
                   
                   
                   
                 t861569 
                   
               
               
                   
                   
                   
                   
                 t861583 
                 162 
               
               
                   
                   
                   
                   
                 t861589 
                   
               
               
                   
                   
                   
                   
                 t861558 
                 134 
               
               
                   
                   
                   
                   
                 t861590 
                   
               
               
                   
                   
                   
                   
                 t861574 
                 164 
               
               
                   
                   
                   
                   
                 t861572 
                   
               
               
                   
                   
                   
                   
                 t861563 
                 112 
               
               
                   
                   
                   
                   
                 t861553 
                   
               
               
                   
                   
                   
                 MKHFSQFSM 
                 t861579 
                 172 
               
               
                   
                   
                   
                 (SEQ ID NO: 
                 t861556 
                   
               
               
                   
                   
                   
                 191) 
                 t861561 
                 166 
               
               
                   
                   
                   
                   
                 t861560 
                   
               
               
                   
                   
                   
                   
                 t861554 
                 159 
               
               
                   
                   
                   
                   
                 t861552 
                   
               
               
                   
                   
                   
                   
                 t861562 
                 167 
               
               
                   
                   
                   
                   
                 t861582 
                   
               
               
                   
                   
                   
                 MKHFNQFSM 
                 t861588 
                 163 
               
               
                   
                   
                   
                 (SEQ ID NO: 
                 t861564 
                   
               
               
                   
                   
                   
                 192) 
                 t861551 
                 105 
               
               
                   
                   
                   
                   
                 t861578 
                   
               
               
                   
                   
                   
                   
                 t861577 
                 126 
               
               
                   
                   
                   
                   
                 t861592 
                   
               
               
                   
                   
                   
                   
                 t861566 
                 155 
               
               
                   
                   
                   
                   
                 t861587 
                   
               
               
                   
               
               
                 P[EQ][TS]A[EAD][QE]IA[GA][VI]V 
                 53 
                 65 
                 PETAEQIAGIVK 
                 t861574 
                 164 
               
               
                 KC (SEQ ID NO: 193) 
                   
                   
                 C (SEQ ID NO: 
                 t861572 
                   
               
               
                   
                   
                   
                 194) 
                 t861551 
                 105 
               
               
                   
                   
                   
                   
                 t861578 
                   
               
               
                   
                   
                   
                   
                 t861577 
                 126 
               
               
                   
                   
                   
                   
                 t861592 
                   
               
               
                   
                   
                   
                   
                 t861566 
                 155 
               
               
                   
                   
                   
                   
                 t861587 
                   
               
               
                   
                   
                   
                 PQSADEIAAVV 
                 t861554 
                 159 
               
               
                   
                   
                   
                 KC (SEQ ID NO: 
                 t861552 
                   
               
               
                   
                   
                   
                 195) 
                 t861588 
                 163 
               
               
                   
                   
                   
                   
                 t861564 
                   
               
               
                   
                   
                   
                   
                 t861562 
                 167 
               
               
                   
                   
                   
                   
                 t861582 
                   
               
               
                   
                   
                   
                 PETAAQIAGVV 
                 t861555 
                 27 
               
               
                   
                   
                   
                 KC (SEQ ID NO: 
                 t861565 
                   
               
               
                   
                   
                   
                 196) 
                 t861571 
                 165 
               
               
                   
                   
                   
                   
                 t861569 
                   
               
               
                   
                   
                   
                   
                 t861583 
                 162 
               
               
                   
                   
                   
                   
                 t861589 
                   
               
               
                   
                   
                   
                 PQSAEEIAAVV 
                 t861579 
                 172 
               
               
                   
                   
                   
                 KC (SEQ ID NO: 
                 t861556 
                   
               
               
                   
                   
                   
                 197) 
                   
                   
               
               
                   
                   
                   
                 PETAEQIAGVV 
                 t861558 
                 134 
               
               
                   
                   
                   
                 KC (SEQ ID NO: 
                 t861590 
                   
               
               
                   
                   
                   
                 198) 
                   
                   
               
               
                   
                   
                   
                 PETAEQIAAVV 
                 t861585 
                 130 
               
               
                   
                   
                   
                 KC (SEQ ID NO: 
                 t861581 
                   
               
               
                   
                   
                   
                 199) 
                   
                   
               
               
                   
               
               
                 RDCL[IV]SA[LV]GGN[SA]A[LH][AV] 
                 10 
                 32 
                 RDCLISAVGGN 
                 t861561 
                 166 
               
               
                 [AV]F[PQ][ND][QE]LL[WY] (SEQ 
                   
                   
                 AAHVAFQDQL 
                 t861560 
                   
               
               
                 ID NO: 200) 
                   
                   
                 LY (SEQ ID NO: 
                 t861562 
                 167 
               
               
                   
                   
                   
                 201) 
                 t861582 
                   
               
               
                   
                   
                   
                 RDCLISALGGN 
                 t861555 
                 27 
               
               
                   
                   
                   
                 SALAVFPNELL 
                 t861565 
                   
               
               
                   
                   
                   
                 W (SEQ ID NO: 
                 t861571 
                 165 
               
               
                   
                   
                   
                 202) 
                 t861569 
                   
               
               
                   
                   
                   
                   
                 t861583 
                 162 
               
               
                   
                   
                   
                   
                 t861589 
                   
               
               
                   
                   
                   
                 RDCLISALGGN 
                 1861558 
                 134 
               
               
                   
                   
                   
                 SALAAFPNELL 
                 t861590 
                   
               
               
                   
                   
                   
                 W (SEQ ID NO: 
                 t861574 
                 164 
               
               
                   
                   
                   
                 203) 
                 t861572 
                   
               
               
                   
                   
                   
                 RDCLISALGGN 
                 t861551 
                 105 
               
               
                   
                   
                   
                 SALAVFPNQLL 
                 t861578 
                   
               
               
                   
                   
                   
                 W (SEQ ID NO: 
                   
                   
               
               
                   
                   
                   
                 204) 
                   
                   
               
               
                   
                   
                   
                 RDCLISALGGN 
                 t861577 
                 126 
               
               
                   
                   
                   
                 SALAAFPNQLL 
                 t861592 
                   
               
               
                   
                   
                   
                 W (SEQ ID NO: 
                   
                   
               
               
                   
                   
                   
                 205) 
                   
                   
               
               
                   
                   
                   
                 RDCLVSALGGN 
                 t861566 
                 155 
               
               
                   
                   
                   
                 SALAAFPNQLL 
                 t861587 
                   
               
               
                   
                   
                   
                 W (SEQ ID NO: 
                   
                   
               
               
                   
                   
                   
                 206) 
                   
                   
               
               
                   
               
               
                 RT[EQ][PQ]APGLAVQYSY (SEQ ID 
                 212 
                 225 
                 RTEPAPGLAVQ 
                 t861555 
                 27 
               
               
                 NO: 207) 
                   
                   
                 YSY (SEQ ID 
                 t861565 
                   
               
               
                   
                   
                   
                 NO: 208) 
                 t861571 
                 165 
               
               
                   
                   
                   
                   
                 t861569 
                   
               
               
                   
                   
                   
                   
                 t861583 
                 162 
               
               
                   
                   
                   
                   
                 t861589 
                   
               
               
                   
                   
                   
                   
                 t861558 
                 134 
               
               
                   
                   
                   
                   
                 t861590 
                   
               
               
                   
                   
                   
                   
                 t861574 
                 164 
               
               
                   
                   
                   
                   
                 t861572 
                   
               
               
                   
                   
                   
                   
                 t861551 
                 105 
               
               
                   
                   
                   
                   
                 t861578 
                   
               
               
                   
                   
                   
                   
                 t861577 
                 126 
               
               
                   
                   
                   
                   
                 t861592 
                   
               
               
                   
                   
                   
                   
                 t861566 
                 155 
               
               
                   
                   
                   
                   
                 t861587 
                   
               
               
                   
                   
                   
                 RTEQAPGLAVQ 
                 t861561 
                 166 
               
               
                   
                   
                   
                 YSY (SEQ ID 
                 t861560 
                   
               
               
                   
                   
                   
                 NO: 209) 
                 t861562 
                 167 
               
               
                   
                   
                   
                   
                 t861582 
                   
               
               
                   
                   
                   
                 RTQPAPGLAVQ 
                 t861563 
                 112 
               
               
                   
                   
                   
                 YSY (SEQ ID 
                 t861553 
                   
               
               
                   
                   
                   
                 NO: 210) 
                   
                   
               
               
                   
               
               
                 WQ[SA]FI[SA][AQ][KE]NLT[RW] 
                 242 
                 259 
                 WQSFISAKNLT 
                 t861555 
                 27 
               
               
                 [QK]FY[NST]NM (SEQ ID NO: 211) 
                   
                   
                 RQFYNNM 
                 t861565 
                   
               
               
                   
                   
                   
                 (SEQ ID NO: 
                 t861571 
                 165 
               
               
                   
                   
                   
                 212) 
                 t861569 
                   
               
               
                   
                   
                   
                   
                 t861583 
                 162 
               
               
                   
                   
                   
                   
                 t861589 
                   
               
               
                   
                   
                   
                   
                 t861558 
                 134 
               
               
                   
                   
                   
                   
                 t861590 
                   
               
               
                   
                   
                   
                   
                 t861574 
                 164 
               
               
                   
                   
                   
                   
                 t861572 
                   
               
               
                   
                   
                   
                   
                 t861551 
                 105 
               
               
                   
                   
                   
                   
                 t861578 
                   
               
               
                   
                   
                   
                   
                 t861577 
                 126 
               
               
                   
                   
                   
                   
                 t861592 
                   
               
               
                   
                   
                   
                   
                 t861566 
                 155 
               
               
                   
                   
                   
                   
                 t861587 
                   
               
               
                   
                   
                   
                 WQSFISAKNLT 
                 t861563 
                 112 
               
               
                   
                   
                   
                 RQFYTNM (SEQ 
                 t861553 
                   
               
               
                   
                   
                   
                 ID NO: 213) 
               
               
                   
               
               
                 *The table includes two strains for every TS, based on data presented in Example 4. For each TS, one strain expressed the TS with signal peptides (top row for each strain) and one strain expressed the TS without signal peptides (bottom row for each strain). 
               
               
                 **The TS SEQ ID NOs provided in the table correspond to the complete protein sequence of each TS. In the context of the screen, for the strains that expressed the TS with signal peptides (top row for each strain), two signal peptides were attached to each TS sequence. At the N-terminus, the N-terminal methionine was removed from each TS sequence, the TS sequence was linked to a signal peptide corresponding to SEQ ID NO: 16, and a methionine residue was added at the N-terminus of SEQ ID NO: 16. At the C-terminus, each TS sequence was linked to a signal peptide corresponding to SEQ ID NO: 17. 
               
            
           
         
       
     
     Example 6: Biosynthesis of Cannabinoids in Engineered  S. cerevisiae  Host Cells 
     The activation of an organic acid to its CoA-thioester and the subsequent condensation of this thioester with a number of malonyl-CoA molecules, or other similar polyketide extender units, represent the first two steps in the biosynthesis of all known cannabinoids. To demonstrate the biosynthesis of CBGA ( FIG.  1   , Formula 8a), CBDA ( FIG.  1   , Formula 9a), THCA ( FIG.  1   , Formula 10a), and/or CBCA ( FIG.  1   , Formula 11a) the cannabinoid biosynthetic pathway shown in  FIG.  1    is assembled in the genome of a prototrophic  S. cerevisiae  CEN.PK host cell wherein each enzyme (R1a-R5a) may be present in one or more copies. For example, the  S. cerevisiae  host cell may express one or more copies of one or more of: an AAE, an OLS, an OAC, a PT, and a TS. 
     The AAE enzyme used may be a naturally occurring or synthetic AAE that is functionally expressed in  S. cerevisiae , or a variant thereof, with activity on hexanaoic acid. The OLS enzyme may be a naturally occurring or synthetic OLS that is functionally expressed in  S. cerevisiae . The OAC enzyme may be a naturally occurring or synthetic OAC that is functionally expressed in  S. cerevisiae . In instances where a bifunctional OLS is used, a separate OAC enzyme may or may not be omitted. The PT enzyme may be a naturally occurring or synthetic PT that is functionally expressed in  S. cerevisiae.    
     A TS enzyme may be a naturally occurring or synthetic TS that is functionally expressed in  S. cerevisiae , or a variant thereof, including a TS from  C. sativa , a variant of a TS from  C. sativa , and/or a TS from a non- Cannabis  species. The TS enzyme may be a TS that produces one or more of CBCA, CBCVA, THCA, THCVA, CBDA, and CBDVA as a majority product. The TS enzyme may comprise one or more of the TS enzymes provided in this disclosure. 
     The cannabinoid fermentation procedure may be similar to the assays described in the Examples above, except that the incubation of production cultures may last from, for example, 48-144 hours and production cultures may be supplemented with, for example, 4% galactose and 1 mM sodium hexanoate every 24 hours. Titers of CBCA, CBCVA, THCA, THCVA, CBDA, and CBDVA are quantified via LC-MS. 
     Sequences Associated with the Disclosure 
     Table 15. Sequences of Candidate CBCASs Described in Example 3* and Example 4** *For the library screen in Example 3, the TS sequences provided in Table 15 were expressed with an N-terminal MFalpha2 signal peptide (SEQ ID NO: 16) and a C-terminal HDEL signal peptide (SEQ ID NO: 17). The methionine residue was removed from the N-terminus of the TS sequences provided in  FIG.  15   . A methionine residue was instead added at the N-terminus of SEQ ID NO: 16.**For the library screen in Example 4, the TS sequences were expressed with and without N-terminal and C-terminal signal peptides. For TS sequences expressed with signal peptides, the same approach as described above for Example 3 was used. 
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                   
                   
                   
                 SEQ 
                   
                 SEQ 
               
               
                 Strain 
                 Strain 
                   
                 ID 
                   
                 ID 
               
               
                 ID 
                 Type 
                 Nucleotide Sequence 
                 NO: 
                 Amino Acid Sequence 
                 NO: 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 t807925 
                 t807925 
                 atgggtaatacgacctctattgccggcagagattgtttg 
                 28 
                 MGNTTSIAGRDCLIS 
                 27 
               
               
                   
                 
                   A. niger 
                 
                 atctcagctttaggtggtaactccgctcttgcagtttttcc 
                   
                 ALGGNSALAVFPNE 
                   
               
               
                   
                 CBCAS 
                 aaacgagttgctatggacagctgacgtacacgaatat 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                 Positive 
                 aatctgaacttgcctgtcactcccgctgctataacctac 
                   
                 PVTPAAITYPETAAQ 
                   
               
               
                   
                 Control 
                 ccagaaaccgccgctcagattgccggtgtggttaagt 
                   
                 IAGVVKCASDYDYK 
                   
               
               
                   
                   
                 gcgcttctgattacgactataaagtccaagcaaggtcc 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 ggaggtcatagtttcggtaattacggcttgggtggagc 
                   
                 LGGADGAVVVWDMK 
                   
               
               
                   
                   
                 tgacggtgcagttgtcgttgatatgaagcacttcactca 
                   
                 HFTQFSMDDETYEA 
                   
               
               
                   
                   
                 attttcgatggacgatgaaacttacgaagctgttatcgg 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 tccaggtacaactttaaacgatgtcgacatcgaattgta 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 caacaacggtaaaagagccatggctcatggtgtatgt 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 ccaaccattaagactggtggtcacttcaccatcggtgg 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 tctaggacctacggctcgtcaatggggtctggctttgg 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 accatgtcgaggaagttgaagttgtgttagctaactcta 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 gcattgttagagcctctaatacacaaaatcaagatgtttt 
                   
                 AANFGIVTEFKVRTE 
                   
               
               
                   
                   
                 ctttgcagtcaagggtgctgctgctaacttcggaatcgt 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 cactgaatttaaagttagaactgaaccagccccaggtt 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 tggctgtacagtactcctataccttcaacttgggttcaac 
                   
                 QSFISAKNLTRQFYN 
                   
               
               
                   
                   
                 tgccgagaaggctcaattcgttaaggattggcaatcttt 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 catttcggctaagaacctaaccagacaattttataataa 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 catggtcatttttgatggtgacataatcttggaaggtttat 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 tcttcggtagcaaggaacaatacgacgccttgggcctt 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 gaagatcacttcgcaccaaagaatccaggtaacatatt 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 ggttttaacagattggctaggcatggtgggtcacgcat 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 tggaagacactattttaaaattggtcggtaataccccaa 
                   
                 EFFEYIANHTAGTPA 
                   
               
               
                   
                   
                 catggttctatgctaagtccttgggttttagacaagaca 
                   
                 WFVTLSLEGGAIND 
                   
               
               
                   
                   
                 ctctgatcccttctgccggtattgacgaatttttcgaata 
                   
                 VAEDATAYAHRDV 
                   
               
               
                   
                   
                 cattgctaaccataccgccggcactcctgcttggtttgt 
                   
                 LFWVQLFMVNPVGP 
                   
               
               
                   
                   
                 tactttgtccttagagggtggtgctatcaacgatgtcgc 
                   
                 ISDTTYEFTDGLYDV 
                   
               
               
                   
                   
                 agaagatgctacggcctatgctcacagagatgttttgtt 
                   
                 LARAVPESVGHAYL 
                   
               
               
                   
                   
                 ctgggtccaactattcatggttaatccagtcggtcctat 
                   
                 GCPDPRMEDAQQK 
                   
               
               
                   
                   
                 ctctgacactacctacgagtttacagacggcttgtacg 
                   
                 YWRTNLPRLQELKE 
                   
               
               
                   
                   
                 atgtgttggcccgtgctgttccagaaagcgtgggacat 
                   
                 ELDPKNTFHHPQGV 
                   
               
               
                   
                   
                 gcttaccttggttgtccagatccaagaatggaagacgc 
                   
                 MPA 
                   
               
               
                   
                   
                 tcaacagaagtattggcgtaccaatttgccccgtctgc 
                   
                   
                   
               
               
                   
                   
                 aagaactaaaggaagagttggatccaaaaaacacctt 
                   
                   
                   
               
               
                   
                   
                 ccatcacccacagggtgttatgccagcttaa 
                   
                   
                   
               
               
                   
               
               
                 t807205 
                 Library 
                 atgggcaatggacaatccaccccactgcaacagtgttt 
                 34 
                 MGNGQSTPLQQCLN 
                 104 
               
               
                   
                   
                 aaacacggtatgcaacggtcgtcttggttgtgtcgcttt 
                   
                 TVCNGRLGCVAFPS 
                   
               
               
                   
                   
                 cccttcggatgcattgtaccaagccgcttgggtgaagc 
                   
                 DALYQAAWVKPYN 
                   
               
               
                   
                   
                 catataatttggacgttcccgttactccaatcgctgtcttt 
                   
                 LDVPVTPIAVFKPSS 
                   
               
               
                   
                   
                 aaaccatcttctactgaagacgttgccggtgctattaag 
                   
                 TEDVAGAIKCAVAS 
                   
               
               
                   
                   
                 tgtgctgtcgcaagcaacgttcatgttcaagctaagtca 
                   
                 NVHVQAKSGGHSY 
                   
               
               
                   
                   
                 ggtggtcacagttacgctaacttcggtttgggtggtca 
                   
                 ANFGLGGQDGELMI 
                   
               
               
                   
                   
                 agatggtgagttaatgatagacttggccaatctacaag 
                   
                 DLANLQDFHMDKTS 
                   
               
               
                   
                   
                 attttcacatggataaaacctcctggcaggctaccttcg 
                   
                 WQATFGAGYRLGD 
                   
               
               
                   
                   
                 gcgctggttacaggttgggtgacctagataagaagttg 
                   
                 LDKKLQANGNRAIA 
                   
               
               
                   
                   
                 caagcaaacggaaacagagccattgctcatggtacat 
                   
                 HGTCPGVGIGGHATI 
                   
               
               
                   
                   
                 gtccaggtgtaggtatcggaggtcacgctactattggt 
                   
                 GGLGPMSRMWGSA 
                   
               
               
                   
                   
                 ggtttaggtcctatgtcaagaatgtggggctctgctctg 
                   
                 LDHVLSVQVVTADG 
                   
               
               
                   
                   
                 gatcatgtcttgtccgttcaagtcgttactgccgacggtt 
                   
                 SIKNASESENSDLFW 
                   
               
               
                   
                   
                 ctatcaaaaatgcatcagaatctgaaaattctgacttgtt 
                   
                 ALRGAGASFGVITKF 
                   
               
               
                   
                   
                 ctgggctttgagaggtgctggtgccagttttggtgtcat 
                   
                 TVKTHPAPGSVVQY 
                   
               
               
                   
                   
                 cacaaagttcactgttaagacccacccagccccaggt 
                   
                 TYKISLGSQAQMAP 
                   
               
               
                   
                   
                 tccgtggttcaatatacttacaaaatttcgttaggatctc 
                   
                 VYAAWQALAGDAK 
                   
               
               
                   
                   
                 aggctcaaatggctcctgtttatgctgcctggcaagca 
                   
                 LDRRFSTLFIAEPLG 
                   
               
               
                   
                   
                 ttagctggtgacgctaagttggatagaagattctcaac 
                   
                 ALITGTFYGTKAEYE 
                   
               
               
                   
                   
                 cctttttattgctgaaccattgggagccttaataacaggt 
                   
                 ATGIAARLPSGGTLD 
                   
               
               
                   
                   
                 actttttacggtacaaaggccgaatatgaagctaccgg 
                   
                 LKLLDWLGSLAHIA 
                   
               
               
                   
                   
                 tattgctgcaagacttccatccggcggtaccttggacct 
                   
                 EVVGLTLGDIPTSFY 
                   
               
               
                   
                   
                 aaagttattggattggttgggtagcttggctcatatcgct 
                   
                 GKSLALREEDMLDR 
                   
               
               
                   
                   
                 gaagttgtcggtctgactttaggtgatattcctacttcttt 
                   
                 TSIDGLFRYMGDAD 
                   
               
               
                   
                   
                 ctacggtaaatcgttggccttgagggaagaagacatg 
                   
                 AGTLLWFVIFNSEG 
                   
               
               
                   
                   
                 ttggatagaacatccatcgacggtttgtttcgttacatgg 
                   
                 GAMADTPAGATAY 
                   
               
               
                   
                   
                 gagatgcagatgctggtacgctattgtggttcgtgatat 
                   
                 PHRDKLIMYQSYVI 
                   
               
               
                   
                   
                 tcaactctgagggtggcgctatggccgatactccagct 
                   
                 GIPTLTKATRDFADG 
                   
               
               
                   
                   
                 ggtgccactgcttaccctcacagagataagttgattatg 
                   
                 VHDRVRMGAPAAN 
                   
               
               
                   
                   
                 tatcaatcttatgtgatcggtattccaacgcttactaaag 
                   
                 STYAGYIDRTLSREA 
                   
               
               
                   
                   
                 caactagagactttgctgacggtgtacacgatagagtc 
                   
                 AQEFYWGAQLPRLR 
                   
               
               
                   
                   
                 cgtatgggagctccagccgctaacagtacctacgctg 
                   
                 EVKKAWDPKDVFH 
                   
               
               
                   
                   
                 gttatatcgacagaaccttatcaagagaagccgctcaa 
                   
                 NPQSVDPAE 
                   
               
               
                   
                   
                 gagttttactggggcgctcagttaccaagactaaggg 
                   
                   
                   
               
               
                   
                   
                 aagttaagaaggcttgggaccctaaagacgttttccat 
                   
                   
                   
               
               
                   
                   
                 aatccacaatccgtcgatccagctgaa 
                   
                   
                   
               
               
                   
               
               
                 t807272 
                 Library 
                 atgggaaatacaacttcaattgcaggcagagattgctt 
                 35 
                 MGNTTSIAGRDCLIS 
                 105 
               
               
                   
                   
                 gatcagtgctctaggtggtaactctgccttagctgtgttt 
                   
                 ALGGNSALAVFPNQ 
                   
               
               
                   
                   
                 cctaaccaacttctgtggacggccgacgtccatgagt 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                   
                 ataatttgaacttgccagttactccagctgctataaccta 
                   
                 PVTPAAITYPETAEQ 
                   
               
               
                   
                   
                 cccagaaaccgctgaacagattgccggtatcgttaaat 
                   
                 IAGIVKCASDYDYK 
                   
               
               
                   
                   
                 gtgcttccgattacgactataaggtccaagctcgttctg 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 gtggtcactcgttcggtaactacggtttaggaggtact 
                   
                 LGGTDGAVVVDMK 
                   
               
               
                   
                   
                 gatggcgcagttgtagttgacatgaagcacttcaacca 
                   
                 HFNQFSMDDQTYEA 
                   
               
               
                   
                   
                 atttagcatggacgatcaaacctacgaagctgtcattg 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 gtcccggtactaccttgaatgatgtagacatcgaattgt 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 ataacaatggtaaaagagctatggcacatggtgtttgt 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 ccaactataaagacaggtggacacttcacaattggtg 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 gtttaggacctactgccagacaatggggtctagctttg 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 gaccacgttgaggaagtcgaagttgtcttggctaattc 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 ctctatcgttagggcttcaaacacccagaaccaagatg 
                   
                 AADFGIVTEFKVRTE 
                   
               
               
                   
                   
                 tgttctttgctgtaaagggtgccgctgctgacttcggtat 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 tgtcacggaatttaaagtcagaactgaaccagcccca 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 ggtcttgccgtccaatactcttacaccttcaacctaggtt 
                   
                 QSFISAKNLTRQFYN 
                   
               
               
                   
                   
                 cgactgctgaaaaggctcaattcgttaaggattggcaa 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 tctttcatttccgccaagaatttgacgagacaattttataa 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 caacatggttatctttgacggtgatattatcttggaaggt 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 ttattctttggcagtaaagaacaatacgatgcattaggtt 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 tggaagaccatttcgctcccaagaatccaggtaatatc 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 ttggttttaaccgattggctaggtatggtgggacatgcc 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 ttagaggacactatattgaagttggttggcaacactcca 
                   
                 QFFEYIANHTAGTPA 
                   
               
               
                   
                   
                 acatggttttacgctaaatccttgggtttcaggcaggat 
                   
                 WFVTLSLEGGAIND 
                   
               
               
                   
                   
                 actttaattccaagtgctggtatcgatcaatttttcgaata 
                   
                 VAEDATAYAHRDV 
                   
               
               
                   
                   
                 cattgctaaccacaccgctggtactcctgcatggttcgt 
                   
                 LFWVQLFMVNPLGP 
                   
               
               
                   
                   
                 aaccttgtctctggagggtggtgccatcaatgacgttg 
                   
                 ISETTYEFTDGLYDV 
                   
               
               
                   
                   
                 ctgaagacgccactgcttatgctcacagagatgtccta 
                   
                 LARAVPESVGHAYL 
                   
               
               
                   
                   
                 ttctgggtccaacttttcatggttaacccattgggtccaa 
                   
                 GCPDPRMENAPQKY 
                   
               
               
                   
                   
                 tttctgaaacaacttacgaatttaccgatggattgtacga 
                   
                 WRTNLPRLQELKEE 
                   
               
               
                   
                   
                 cgtgctagcacgtgcagttccagaaagcgtcggtcac 
                   
                 LDPKNTFHHPQGVIP 
                   
               
               
                   
                   
                 gcttatttgggttgtcctgatccaagaatggagaacgc 
                   
                 A 
                   
               
               
                   
                   
                 ccctcaaaagtattggagaacgaatcttccaagacttc 
                   
                   
                   
               
               
                   
                   
                 aagaactgaaggaagagttggatccaaagaacacttt 
                   
                   
                   
               
               
                   
                   
                 tcatcatcctcaaggtgtcatcccagct 
                   
                   
                   
               
               
                   
               
               
                 t807301 
                 Library 
                 atgggaaacacgaccagcatagctggtcgtgactgtc 
                 36 
                 MGNTTSIAGRDCLIS 
                 106 
               
               
                   
                   
                 tgatctctgccttgggtggcaattcagcattagctgcttt 
                   
                 ALGGNSALAAFPNQ 
                   
               
               
                   
                   
                 cccaaaccaactattgtggactgccgatgtccacgaat 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                   
                 acaaccttaatttgcctgtgacaccagctgctattactta 
                   
                 PVTPAAITYPETAEQ 
                   
               
               
                   
                   
                 tcccgagactgccgaacagatcgctggtattgttaagt 
                   
                 IAGIVKCASDYDYK 
                   
               
               
                   
                   
                 gcgcctctgattacgactacaaagtacaagctagatcg 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 ggtggtcattcctttggtaattatggtttgggtggtaccg 
                   
                 LGGTDGAVVVDMK 
                   
               
               
                   
                   
                 atggtgctgtcgttgttgacatgaagcacttcaaccaat 
                   
                 HFNQFSMDDQTYEA 
                   
               
               
                   
                   
                 tttctatggatgatcaaacctacgaagcagtcattggac 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 caggtactaccttaaacgacgtagatatcgaattgtac 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 aataacggtaaaagagctatggcccatggtgtgtgtcc 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 aacaatcaagactggaggtcacttcaccattggcggc 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 ttgggtccaactgctagacaatggggtttagctttagac 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 catgttgaagaggttgaagttgtcttggccaactccagt 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 attgttagggcatctaatactcaaaaccaggacgttttct 
                   
                 AADFGIVTEFKVRTE 
                   
               
               
                   
                   
                 ttgctgtcaagggtgctgctgctgacttcggtatcgtga 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 ccgaatttaaagttagaacagaacctgccccaggtttg 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 gccgtccaatattcctacaccttcaatcttggttcaactg 
                   
                 QYFISAKNLTRQFYN 
                   
               
               
                   
                   
                 ctgaaaaggcacaattcgtaaaggattggcaatacttc 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 atctctgctaaaaacctaacaagacaattttacaacaac 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 atggttatttttgacggtgatataattttggaaggtctgtt 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 cttcggtagtaaggaacaatatgacgccttgggtttgg 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 aggatcactttgctcccaagaatccaggaaatattttag 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 tcctaacggattggttgggcatggttggtcacgcatta 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 gaagatactattctaaaattggtcggtaacacgccaac 
                   
                 EFFEYIANHTAGTPA 
                   
               
               
                   
                   
                 ttggttctatgctaagtccttgggttttcgtcaggacacc 
                   
                 WFVTLSLEGGAIND 
                   
               
               
                   
                   
                 cttatcccttctgctggtattgatgaatttttcgagtacat 
                   
                 VAEDATAYAHRDV 
                   
               
               
                   
                   
                 cgctaatcataccgccggtactccagcttggtttgttac 
                   
                 LFWVQLFMVNPLGP 
                   
               
               
                   
                   
                 tttatctttggaaggtggagctatcaacgacgtcgctga 
                   
                 ISETTYEFTDGLYDV 
                   
               
               
                   
                   
                 agatgccacagcatacgcacatagagatgtgttattct 
                   
                 LARAVPESVGHAYL 
                   
               
               
                   
                   
                 gggttcaattgttcatggttaaccctcttggtccaatttca 
                   
                 GCPDPRMENAPQKY 
                   
               
               
                   
                   
                 gaaacaacttatgaatttaccgatggattgtacgacgtt 
                   
                 WRTNLPRLQELKEE 
                   
               
               
                   
                   
                 ttagctagagctgtcccagaatctgtaggtcacgcttac 
                   
                 LDPKNTFHHPQGVIP 
                   
               
               
                   
                   
                 ttgggttgtccagacccaagaatggagaacgcacctc 
                   
                 A 
                   
               
               
                   
                   
                 aaaagtattggaggacaaacttgccaagactacagga 
                   
                   
                   
               
               
                   
                   
                 actgaaagaggaattggaccccaagaatacttttcacc 
                   
                   
                   
               
               
                   
                   
                 atccacaaggtgttatcccagct 
                   
                   
                   
               
               
                   
               
               
                 t807677 
                 Library 
                 atggatccaatcgaggacgccattttgcagtgcttaag 
                 37 
                 MDPIEDAILQCLSLH 
                 107 
               
               
                   
                   
                 cctacacagtgacccttcgcatccaatatcaggcgtaa 
                   
                 SDPSHPISGVTYFPN 
                   
               
               
                   
                   
                 cgtatttccccaatacaccatcttacattcctatcctgca 
                   
                 TPSYIPILHSYIRNLR 
                   
               
               
                   
                   
                 ctcctacattcgtaaccttagatttacctctccatccacta 
                   
                 FTSPSTRKPLFIVAPT 
                   
               
               
                   
                   
                 gaaaaccattgttcatcgttgctccaactcatatatctca 
                   
                 HISHIQASIICCKSFQ 
                   
               
               
                   
                   
                 catccaagcatcaattatctgttgtaagtcttttcaattgc 
                   
                 LQIRIRSGGHDYDGL 
                   
               
               
                   
                   
                 aaattaggattagaagtggaggtcacgattatgatggtt 
                   
                 SYVSQSPFAIMDMF 
                   
               
               
                   
                   
                 tgtcctacgtcagccaatctcccttcgctattatggacat 
                   
                 AMRSVEVNLEDETV 
                   
               
               
                   
                   
                 gttcgctatgagatccgttgaagtcaacttagaagatg 
                   
                 WVDSGSTIGELYHGI 
                   
               
               
                   
                   
                 aaaccgtttgggttgactctggttccactatcggtgaatt 
                   
                 AERSKVHGFPAGVC 
                   
               
               
                   
                   
                 gtaccatggtattgccgaaagatctaaggtccatggttt 
                   
                 HSVGVGGHFSGGGY 
                   
               
               
                   
                   
                 cccagctggtgtgtgtcactcagttggcgtcggtggac 
                   
                 GNMMRKFGLSVDH 
                   
               
               
                   
                   
                 acttttccggtggtggttatggtaatatgatgagaaagtt 
                   
                 VLDAVIVDAEGRVL 
                   
               
               
                   
                   
                 cggtttgtctgtggaccatgttctggatgctgttatcgtt 
                   
                 DRKKMGEDLFWGIR 
                   
               
               
                   
                   
                 gatgcagaaggccgtgtcttagacagaaaaaagatg 
                   
                 GGGGASFGVIVSWR 
                   
               
               
                   
                   
                 ggtgaagacctattctggggtataagaggtggtggtg 
                   
                 IKLVPVPEVVTVFRV 
                   
               
               
                   
                   
                 gcgcttcgtttggtgttatcgtcagttggagaattaaatt 
                   
                 LKTLEQGATDVVHR 
                   
               
               
                   
                   
                 ggtcccagtgcctgaggttgtaaccgtcttccgtgtttt 
                   
                 WQYVADNIHDDLFI 
                   
               
               
                   
                   
                 gaagaccttggaacaaggtgccacagatgtcgttcac 
                   
                 RVVLSPVKRKGQKT 
                   
               
               
                   
                   
                 agatggcaatacgtcgccgacaacatccacgatgact 
                   
                 IRAKFNALFLGNAQ 
                   
               
               
                   
                   
                 tatttattagagttgttctatctccagttaagagaaaaggt 
                   
                 ELLRVMSDSFPELGL 
                   
               
               
                   
                   
                 cagaagactatcagagctaagtttaatgctttgttcttgg 
                   
                 VGEDCIEMSWIDSV 
                   
               
               
                   
                   
                 gtaacgctcaagaattactgcgtgtcatgtctgattctttt 
                   
                 LFWDNFPVGTSVDV 
                   
               
               
                   
                   
                 ccagaattgggattagtgggtgaagactgtatcgagat 
                   
                 LLQRHDTPEKFLKK 
                   
               
               
                   
                   
                 gagctggattgactccgtattgttctgggataactttcca 
                   
                 KSDYVQQPISKTGLE 
                   
               
               
                   
                   
                 gtaggtacatctgtagatgttttattgcagcgtcacgac 
                   
                 GVWNKMMELEKPV 
                   
               
               
                   
                   
                 actcctgaaaaattcttgaagaagaaatccgattacgtt 
                   
                 LTLNPYGGRMGEISE 
                   
               
               
                   
                   
                 caacaaccaatctctaagactggattagaaggtgtttg 
                   
                 MEIPFPHRAGNLYKI 
                   
               
               
                   
                   
                 gaataaaatgatggaacttgaaaagccagtgttgacct 
                   
                 QYSVNWKEEGEDV 
                   
               
               
                   
                   
                 tgaatccatatggtggtagaatgggtgaaataagtgaa 
                   
                 ANRYLDLIRMLYDY 
                   
               
               
                   
                   
                 atggaaattccttttccacatagagctggtaacttgtaca 
                   
                 MTPYVSKSPRSSYL 
                   
               
               
                   
                   
                 agatccaatactcggtcaactggaaggaggaaggtg 
                   
                 NYRDVDIGVNGPGN 
                   
               
               
                   
                   
                 aggatgttgcaaacaggtatcttgacctgattagaatgt 
                   
                 ATYAEARVWGEKY 
                   
               
               
                   
                   
                 tatacgactacatgaccccatatgtttcaaagtccccca 
                   
                 FKRNFDRLVEVKTR 
                   
               
               
                   
                   
                 gatcaagttatttgaactacagagatgtcgatatagga 
                   
                 VDPSNFFRYEQSIPS 
                   
               
               
                   
                   
                 gtcaatggtccaggcaatgccacttatgctgaagctag 
                   
                 LAASSLGIMSE 
                   
               
               
                   
                   
                 agtctggggagagaaatacttcaagagaaactttgac 
                   
                   
                   
               
               
                   
                   
                 agattggttgaagtcaaaactagggttgatccaagtaa 
                   
                   
                   
               
               
                   
                   
                 cttcttcaggtacgaacaatctataccttccttggccgct 
                   
                   
                   
               
               
                   
                   
                 tcgagcctaggtattatgtcggaa 
                   
                   
                   
               
               
                   
               
               
                 t807764 
                 Library 
                 atggtccacaatatattactacttggtttgatgccactgtt 
                 38 
                 MVHNILLLGLMPLL 
                 108 
               
               
                   
                   
                 ggttcgtgcatcacctttgccaatttatcataactacccc 
                   
                 VRASPLPIYHNYPPQ 
                   
               
               
                   
                   
                 ccacaatcgactatcaacgactgcttgcaggccgctg 
                   
                 STINDCLQAADVPAI 
                   
               
               
                   
                   
                 atgttccagctatcttacaaagctctgcttcctttgatgc 
                   
                 LQSSASFDALSQPLN 
                   
               
               
                   
                   
                 cttgagtcaacctctaaattccagattaaaatctaagcc 
                   
                 SRLKSKPAVITIPTTA 
                   
               
               
                   
                   
                 agctgtgattacaatccctacgaccgctttgcacgtca 
                   
                 LHVSSAVKCAAQFK 
                   
               
               
                   
                   
                 gttctgctgttaagtgtgccgcacaattcaagctgaaa 
                   
                 LKVTPRGGGHSYNA 
                   
               
               
                   
                   
                 gtaactccaagaggcggtggacattcttacaacgcac 
                   
                 QSLGDGAVVIDMQQ 
                   
               
               
                   
                   
                 aatccttaggtgacggtgctgtcgttattgatatgcaac 
                   
                 FHDVVYDSKTQLAR 
                   
               
               
                   
                   
                 agttccacgacgttgtctacgactctaagactcaacta 
                   
                 IGGGARLGNVAQKL 
                   
               
               
                   
                   
                 gctaggattggtggtggagctagattgggtaacgttgc 
                   
                 YDQGKRAMPHGTC 
                   
               
               
                   
                   
                 ccaaaaattgtatgatcaaggtaagagagctatgccac 
                   
                 PDVGIGGHSAGGFG 
                   
               
               
                   
                   
                 atggtacctgtccagatgtcggtattggcggtcactcc 
                   
                 WTSRQWGITVDHID 
                   
               
               
                   
                   
                 gccggtggttttggttggacctcacgtcagtggggtat 
                   
                 EVEVVTADGSIRRA 
                   
               
               
                   
                   
                 cactgtagatcacatagacgaggttgaagtggtaaca 
                   
                 NKDQNSDLFWALR 
                   
               
               
                   
                   
                 gctgacggttctatcagaagagctaataaggatcaaa 
                   
                 GAAPSFGVITNFWFS 
                   
               
               
                   
                   
                 attccgatttgttctgggcattgagaggagctgccccat 
                   
                 TLEAPDSNVIYSYKF 
                   
               
               
                   
                   
                 cgttcggtgttattactaacttttggttttctaccttggaag 
                   
                 TGLSLDEISTALLEV 
                   
               
               
                   
                   
                 ctcctgattctaacgttatttacagttataagttcactggtt 
                   
                 QKFGQTAPKEVGML 
                   
               
               
                   
                   
                 tatctttagacgaaatcagtacagctttgttggaagtgc 
                   
                 IQILDNGSGFRLYGT 
                   
               
               
                   
                   
                 aaaagttcggtcaaaccgctcccaaagaagtcggcat 
                   
                 YYNTTRQQFDNLFG 
                   
               
               
                   
                   
                 gcttatccaaatattagacaatggttctggtttcagattgt 
                   
                 QLLQRLPSPGNSAEV 
                   
               
               
                   
                   
                 acggtacgtactataacactacccgtcaacaatttgata 
                   
                 SVKGWIDSLIFASGG 
                   
               
               
                   
                   
                 atttattcggccaacttttgcaaagattgccatccccag 
                   
                 SKGLTVPELGGTNQ 
                   
               
               
                   
                   
                 gtaacagcgctgaggtttctgtcaagggttggattgac 
                   
                 HSSFYTKSLMTAQD 
                   
               
               
                   
                   
                 tcgttgatatttgcctctggcggtagcaagggtcttact 
                   
                 YPLTLDSIKSVFKYA 
                   
               
               
                   
                   
                 gttccagaactgggtggaactaaccagcattcttccttt 
                   
                 MNQGRAATERGLP 
                   
               
               
                   
                   
                 tacacaaaatcattgatgactgctcaagattacccatta 
                   
                 WMVFISLLGGRYST 
                   
               
               
                   
                   
                 accctggattcaattaagtccgtgttcaagtatgccatg 
                   
                 LPTPSAASDNSFYGR 
                   
               
               
                   
                   
                 aaccaaggtagagccgccaccgaaaggggtctacc 
                   
                 NTLWAFSFTAYLGN 
                   
               
               
                   
                   
                 atggatggtatttatctctttgttgggtggtagatatagc 
                   
                 VTEQSNRDSIYFLNG 
                   
               
               
                   
                   
                 actctaccaacgccttccgctgcttcagataactctttct 
                   
                 FDTSVRRSVDTAYIN 
                   
               
               
                   
                   
                 acggcagaaacactttgtgggctttttctttcaccgctta 
                   
                 GHDTEYSREEAHRL 
                   
               
               
                   
                   
                 cctaggtaacgtcacagaacaaagcaatagagactca 
                   
                 YYGDKYQRLSVLKK 
                   
               
               
                   
                   
                 atttacttcttgaatggtttcgacacttccgtaagaagat 
                   
                 QWDPEQVFWYPQSI 
                   
               
               
                   
                   
                 ccgttgacaccgcttacatcaacggtcacgatactgaa 
                   
                 DPAN 
                   
               
               
                   
                   
                 tattcgagagaagaagcacatagattatactacggtga 
                   
                   
                   
               
               
                   
                   
                 caaatatcaaaggttgtctgtcttaaagaagcaatggg 
                   
                   
                   
               
               
                   
                   
                 atcctgagcaagttttctggtatccacaatccatcgacc 
                   
                   
                   
               
               
                   
                   
                 ccgccaat 
                   
                   
                   
               
               
                   
               
               
                 t807774 
                 Library 
                 atgggtaacacaacttcaatcgcagctggcagggatt 
                 39 
                 MGNTTSIAAGRDCL 
                 109 
               
               
                   
                   
                 gcttactgtccgccgtcggaggtaatcacgctcatgtt 
                   
                 LSAVGGNHAHVAFQ 
                   
               
               
                   
                   
                 gcttttcaggaccaattgctatatcaagctaccgcagtg 
                   
                 DQLLYQATAVEPYN 
                   
               
               
                   
                   
                 gaaccatacaacttgaatattcccgttacgccagccgc 
                   
                 LNIPVTPAAVTYPQS 
                   
               
               
                   
                   
                 tgttacctaccctcaatcggctgatgaggttgccgctgt 
                   
                 ADEVAAVVKCAAD 
                   
               
               
                   
                   
                 cgtaaaatgtgcagccgactatggttacaaggtgcaa 
                   
                 YGYKVQARSGGHSF 
                   
               
               
                   
                   
                 gctagaagcggtggtcacagtttcggtaactacggttt 
                   
                 GNYGLGGEDGAIVV 
                   
               
               
                   
                   
                 aggcggtgaagacggtgctatagtcgttgatatgaag 
                   
                 DMKHFDQFSMDEST 
                   
               
               
                   
                   
                 catttcgatcaattttctatggacgaatctacttatactgc 
                   
                 YTATIGPGITLGDLD 
                   
               
               
                   
                   
                 tactattggtccaggtatcaccttgggagacttggatac 
                   
                 TALYNAGHRAMAH 
                   
               
               
                   
                   
                 cgccctatacaatgctggccatagagccatggctcac 
                   
                 GICPTIRTGGHLTIGG 
                   
               
               
                   
                   
                 ggtatttgtccaacaattcgtactggtggtcaccttacc 
                   
                 LGPTARQWGLALDH 
                   
               
               
                   
                   
                 atcggaggtttgggtccaactgctagacaatggggttt 
                   
                 VEEVEVVLANSSIVR 
                   
               
               
                   
                   
                 ggccttagatcacgttgaagaagtcgaagttgtcttgg 
                   
                 ASDTQNQEILFAVK 
                   
               
               
                   
                   
                 caaacagctccatcgtcagagcatcagacactcagaa 
                   
                 GAAASFGIVTEFKVR 
                   
               
               
                   
                   
                 ccaagagatcttgttcgctgttaagggtgctgctgcttc 
                   
                 TEEAPGLAVQYSFTF 
                   
               
               
                   
                   
                 tttcggtatagtaactgaatttaaagttagaacagaaga 
                   
                 NLGTAAEKAKLVKD 
                   
               
               
                   
                   
                 agctcctggtcttgccgtccaatactccttcaccttcaa 
                   
                 WQAFIAQEDLTWKF 
                   
               
               
                   
                   
                 cttaggtacagctgccgagaaggctaaattggttaag 
                   
                 YSNMNIIDGQIILEGI 
                   
               
               
                   
                   
                 gactggcaagcttttattgcacaagaagatttgacgtg 
                   
                 YFGSKAEYDALGLE 
                   
               
               
                   
                   
                 gaagttctactctaacatgaatattatcgacggtcaaatt 
                   
                 EKFPTSEPGTVLVLT 
                   
               
               
                   
                   
                 atcctagaaggcatatatttcggttctaaggctgaatac 
                   
                 DWLGMVGHGLEDV 
                   
               
               
                   
                   
                 gatgccttaggtttggaggaaaagtttccaaccagtga 
                   
                 ILRLVGNAPTWFYA 
                   
               
               
                   
                   
                 accaggcactgttttagtcttgacggactggctgggtat 
                   
                 KSLGFAPRALIPDSAI 
                   
               
               
                   
                   
                 ggttggtcatggtttggaagatgttattttgcgtttagtag 
                   
                 DDFFEYIHKNNPGT 
                   
               
               
                   
                   
                 gcaatgctccaacttggttctatgctaaatctttaggtttt 
                   
                 VSWFVTLSLEGGAI 
                   
               
               
                   
                   
                 gctcccagggcattgatcccagattccgctattgacga 
                   
                 NKVPEDATAYGHRD 
                   
               
               
                   
                   
                 tttcttcgaatacattcacaagaacaatcctggtaccgtt 
                   
                 VLFWVQIFMINPLGP 
                   
               
               
                   
                   
                 agttggttcgtcacactatcgttggaaggtggtgcaata 
                   
                 VSQTIYDFADGLYD 
                   
               
               
                   
                   
                 aacaaggtgccagaagatgccactgcttacggacata 
                   
                 VLAKAVPESAGHAY 
                   
               
               
                   
                   
                 gagatgttttgttttgggttcaaatctttatgattaaccca 
                   
                 LGCPDPRMPNAQQA 
                   
               
               
                   
                   
                 ctaggtcctgtttctcagaccatttacgactttgccgac 
                   
                 YWRNNLPRLEELKG 
                   
               
               
                   
                   
                 ggtctttatgacgttctggctaaagccgtccccgaatcc 
                   
                 DLDPKDIFHNPQGV 
                   
               
               
                   
                   
                 gcaggtcatgcttatttgggctgtccagacccaagaat 
                   
                 MVVS 
                   
               
               
                   
                   
                 gccaaatgctcaacaagcctactggagaaataacttg 
                   
                   
                   
               
               
                   
                   
                 ccaagactagaggaattgaagggtgacttagatccaa 
                   
                   
                   
               
               
                   
                   
                 aggatatcttccacaacccacagggtgtcatggttgtct 
                   
                   
                   
               
               
                   
                   
                 ct 
                   
                   
                   
               
               
                   
               
               
                 t807810 
                 Library 
                 atgggtaacactaccagcattgccggccgtgactgcc 
                 40 
                 MGNTTSIAGRDCLV 
                 110 
               
               
                   
                   
                 tagtttccgctttgggtggtaatgcaggtctggtggcttt 
                   
                 SALGGNAGLVAFQS 
                   
               
               
                   
                   
                 tcagtcacaaccattataccaaacaaccgctgtccatg 
                   
                 QPLYQTTAVHEYNL 
                   
               
               
                   
                   
                 agtataaccttaacatacccgttactccagccgctatcg 
                   
                 NIPVTPAAIAYPETA 
                   
               
               
                   
                   
                 cttaccctgaaactgccgaacaaattgctgctgtcgta 
                   
                 EQIAAVVKCASEYD 
                   
               
               
                   
                   
                 aaatgtgcatcggaatatgattacaaggttcaagcaag 
                   
                 YKVQARSGGHSFGN 
                   
               
               
                   
                   
                 atccggtggtcactctttcggaaattacggtttgggtgg 
                   
                 YGLGGTDGAVVVD 
                   
               
               
                   
                   
                 tacggatggtgctgttgtggtcgacatgaagcacttca 
                   
                 MKHFNQFSMDDQT 
                   
               
               
                   
                   
                 accaatttagtatggacgatcaaacctatgaagctgtta 
                   
                 YEAVIGPGTTLGDV 
                   
               
               
                   
                   
                 tcggcccaggtactactttgggcgacgtcgatactga 
                   
                 DTELYNNGKRAMA 
                   
               
               
                   
                   
                 gctatacaataacggtaagagagccatggcccatggt 
                   
                 HGICPTISTGGHFTM 
                   
               
               
                   
                   
                 atctgtccaacaatttctaccggtggccacttcacgatg 
                   
                 GGLGPTARQWGLAL 
                   
               
               
                   
                   
                 ggtggtttaggtccaacggctagacagtggggtttgg 
                   
                 DHVEEVEVVLANSSI 
                   
               
               
                   
                   
                 cattggatcacgttgaagaagtagaagtcgttttggcta 
                   
                 VRASNTQNQEVFFA 
                   
               
               
                   
                   
                 attcttctatcgtgagggcttccaacacccaaaaccaa 
                   
                 VKGAAASFGIVTEF 
                   
               
               
                   
                   
                 gaagttttctttgccgttaaaggagctgctgcttcatttg 
                   
                 KVRTQPAPGLAVQY 
                   
               
               
                   
                   
                 gtattgtcaccgaatttaaggttagaactcaaccagctc 
                   
                 SYTFNLGSSAEKAQF 
                   
               
               
                   
                   
                 ctggattggctgtccaatactcttacactttcaacttggg 
                   
                 VKDWQSFISAKNLT 
                   
               
               
                   
                   
                 ttcgagtgctgaaaaggctcaattcgtcaaggattggc 
                   
                 RQFYTNMVIFDGDII 
                   
               
               
                   
                   
                 aatctttcatctctgctaaaaacttaacaagacagttttat 
                   
                 LEGLFFGSKEQYEAL 
                   
               
               
                   
                   
                 accaatatggttatattcgacggcgacattattttggaa 
                   
                 GLEERFVPKNPGNIL 
                   
               
               
                   
                   
                 ggtctgttctttggtagcaaggagcaatacgaagccct 
                   
                 VLTDWLGMVGHAL 
                   
               
               
                   
                   
                 tggtttggaagaacgtttcgtcccaaagaatcctggta 
                   
                 EDTILRLVGNTPTWF 
                   
               
               
                   
                   
                 acattcttgttttaactgattggttgggtatggttggtcat 
                   
                 YAKSLGFTPDTLIPS 
                   
               
               
                   
                   
                 gctttggaggacactatcttaagattagtcggtaacacc 
                   
                 SGIDEFFEYIENNKA 
                   
               
               
                   
                   
                 ccaacctggttctacgcaaaatccctaggcttcacccc 
                   
                 GTSTWFVTLSLEGG 
                   
               
               
                   
                   
                 agatactttgataccctcctcaggtattgatgaatttttcg 
                   
                 AINDVPADTTAYGH 
                   
               
               
                   
                   
                 aatatatcgagaataataaggccggtacctctacatgg 
                   
                 RDVLFWVQIFMVSP 
                   
               
               
                   
                   
                 tttgtaacattatctcttgaaggtggtgccatcaacgac 
                   
                 TGPVSSTTYDFADG 
                   
               
               
                   
                   
                 gttccagctgatacgacagcatacggtcacagagatg 
                   
                 LYNVLTKAVPESEG 
                   
               
               
                   
                   
                 tattgttttgggtccagatattcatggtttccccaactggt 
                   
                 HAYLGCPDPKMAN 
                   
               
               
                   
                   
                 ccagtttcctctacaacttacgattttgctgacggcttgt 
                   
                 AQQKYWRQNLPRL 
                   
               
               
                   
                   
                 ataacgtgttgactaaggcagttcctgaaagcgaaggt 
                   
                 EELKATLDPKDTFH 
                   
               
               
                   
                   
                 catgcttacttgggatgtcctgaccctaagatggctaac 
                   
                 NPQGILPV 
                   
               
               
                   
                   
                 gcccaacaaaaatattggagacaaaatctaccaagac 
                   
                   
                   
               
               
                   
                   
                 tggaggaattgaaagctactcttgacccaaaggatac 
                   
                   
                   
               
               
                   
                   
                 ctttcataacccccaaggtatcttgccagta 
                   
                   
                   
               
               
                   
               
               
                 t807822 
                 Library 
                 atgaatccttctataccctcaagctccatgggtaacaca 
                 41 
                 MNPSIPSSSMGNTTS 
                 111 
               
               
                   
                   
                 acgtctatcgctggacgtgactgtttagttagtgccctg 
                   
                 IAGRDCLVSALGGN 
                   
               
               
                   
                   
                 ggtggtaacgctggtttggtagcattccaaaatcagcc 
                   
                 AGLVAFQNQPLYQT 
                   
               
               
                   
                   
                 actataccaaaccactgctgtgcacgagtataacttaa 
                   
                 TAVHEYNLNIPVTPA 
                   
               
               
                   
                   
                 acattccagtcactccagccgctattacctacccagaa 
                   
                 AITYPETAEQIAAVV 
                   
               
               
                   
                   
                 actgctgaacaaatcgccgctgttgtcaaatgcgcatc 
                   
                 KCASQYDYKVQARS 
                   
               
               
                   
                   
                 ccaatatgattacaaggttcaagctaggtctggtggcc 
                   
                 GGHSFGNYGLGGTD 
                   
               
               
                   
                   
                 attcgtttggtaactacggtcttggtggcaccgatggtg 
                   
                 GAVVVDMKYFNQF 
                   
               
               
                   
                   
                 ctgttgtcgttgacatgaagtatttcaatcaattttccatg 
                   
                 SMDDQTYEAVIGPG 
                   
               
               
                   
                   
                 gacgatcagacatacgaagcagttattggtcctggtac 
                   
                 TTLGDVDVELYNNG 
                   
               
               
                   
                   
                 taccttgggagatgtcgatgtcgaattgtataacaatgg 
                   
                 KRAMAHGVCPTIST 
                   
               
               
                   
                   
                 taaaagagctatggcccacggtgtgtgtccaactatct 
                   
                 GGHFTMGGLGPTAR 
                   
               
               
                   
                   
                 ctaccggtggccatttcactatgggtggtttaggtccaa 
                   
                 QWGLALDHVEEVE 
                   
               
               
                   
                   
                 cagctagacaatggggattggccttggaccacgttga 
                   
                 VVLANSSIVRASNTQ 
                   
               
               
                   
                   
                 ggaagttgaagtggttctagctaattcatctatcgtcag 
                   
                 NQEVFFAVKGAAAS 
                   
               
               
                   
                   
                 agcttcaaacacccaaaaccaagaagttttctttgccgt 
                   
                 FGIVTEFKVRTQPAP 
                   
               
               
                   
                   
                 aaagggtgctgctgcctcgtttggtattgtcaccgaatt 
                   
                 GIAVQYSYTFNLGSS 
                   
               
               
                   
                   
                 taaggttagaactcagcctgcaccaggtattgctgtgc 
                   
                 AEKAQFIKDWQSFV 
                   
               
               
                   
                   
                 aatactcttacactttcaacttgggttcctccgcagaaa 
                   
                 SAKNLTRQFYTNMV 
                   
               
               
                   
                   
                 aagctcaattcatcaaggactggcaatctttcgtttctgc 
                   
                 IFDGDIILEGLFFGSK 
                   
               
               
                   
                   
                 taagaatcttacgagacaattctacactaacatggtcat 
                   
                 EQYEALGLEERFVP 
                   
               
               
                   
                   
                 atttgacggtgatattattttggaaggattgttcttcggta 
                   
                 KNPGNIMVLTDWLG 
                   
               
               
                   
                   
                 gtaaagagcaatatgaagccttgggtttagaagaaag 
                   
                 MVGHALEDTILRLV 
                   
               
               
                   
                   
                 gtttgtccctaagaacccaggtaatatcatggttctaac 
                   
                 GNTPTWFYAKSLGF 
                   
               
               
                   
                   
                 agattggttgggtatggttggccatgctctggaagata 
                   
                 TPDTLIPSSGIDEFFE 
                   
               
               
                   
                   
                 cgattttgagattggtaggtaatacgccaacttggttcta 
                   
                 YIENNKAGTSTWFV 
                   
               
               
                   
                   
                 cgctaagtccctgggttttactccagacacattaatccc 
                   
                 TLSLEGGAINDVPAD 
                   
               
               
                   
                   
                 cagcagcggtattgacgaatttttcgaatatatagaaaa 
                   
                 ATAYGHRDVLFWV 
                   
               
               
                   
                   
                 caacaaggcaggaaccagtacttggttcgtcaccctat 
                   
                 QIFMVSPTGPVSSTT 
                   
               
               
                   
                   
                 ctttggagggtggtgctattaacgatgtgccagcagat 
                   
                 YDFADGLYNVLTKA 
                   
               
               
                   
                   
                 gctaccgcttacggtcacagagatgttttattctgggta 
                   
                 VPESEGHAYLGCPD 
                   
               
               
                   
                   
                 cagatttttatggtttctcccactggtccagtttcctccac 
                   
                 PKMANAQQKYWRQ 
                   
               
               
                   
                   
                 tacctacgactttgccgacggcttgtacaatgtcttgac 
                   
                 NLPRLEELKETLDPK 
                   
               
               
                   
                   
                 taaggctgttccagagtctgaaggccacgcctatttgg 
                   
                 DTFHNPQGILPA 
                   
               
               
                   
                   
                 gctgtccagaccctaaaatggctaacgctcaacaaaa 
                   
                   
                   
               
               
                   
                   
                 gtactggagacaaaacttgccacgtctggaagagctt 
                   
                   
                   
               
               
                   
                   
                 aaggaaacattagatccaaaagacactttccacaatcc 
                   
                   
                   
               
               
                   
                   
                 tcaaggtatcttgccagca 
                   
                   
                   
               
               
                   
               
               
                 1807854 
                 Library 
                 atggcccgtgtccccgcactgttcttgggcctatcttca 
                 42 
                 MARVPALFLGLSSV 
                 112 
               
               
                   
                   
                 gtgtgtttgttagttttcttttgcagtttgattaacccaacc 
                   
                 CLLVFFCSLINPTTPL 
                   
               
               
                   
                   
                 actcctttacatcagcacggtaagcaaacatctatcgct 
                   
                 HQHGKQTSIAGRDC 
                   
               
               
                   
                   
                 ggtagggattgtcttgtatccgctttgggtggaaatact 
                   
                 LVSALGGNTGLVAF 
                   
               
               
                   
                   
                 ggtttagttgcttttcaagaccaattgttgtaccaaacca 
                   
                 QDQLLYQTTAVHEY 
                   
               
               
                   
                   
                 cggctgtccacgagtataacctgaacataccagttact 
                   
                 NLNIPVTPAAITYPE 
                   
               
               
                   
                   
                 ccagccgctatcacctacccagaaacctcggaacaa 
                   
                 TSEQIAAVVKCASE 
                   
               
               
                   
                   
                 attgctgccgttgttaaatgtgcatctgaatacgattata 
                   
                 YDYKVQARSGGHSF 
                   
               
               
                   
                   
                 aggtccaggccagatccggtggtcatagcttcggtaa 
                   
                 GNYGLGGADGAVV 
                   
               
               
                   
                   
                 ttacggattgggtggtgctgacggtgctgttgtcgttga 
                   
                 VDMKHFTQFSMDE 
                   
               
               
                   
                   
                 tatgaagcacttcactcaattttctatggacgaacaaac 
                   
                 QTYEAVIGPGTTLG 
                   
               
               
                   
                   
                 ctatgaagctgtaattggcccaggtacaactctaggtg 
                   
                 DVDTELYNNGKRA 
                   
               
               
                   
                   
                 atgtggataccgagttgtacaacaacggtaaaagagc 
                   
                 MAHGICPTISTGGHF 
                   
               
               
                   
                   
                 tatggcccatggtatctgtcctactatctccactggcgg 
                   
                 TMGGLGPTARQWG 
                   
               
               
                   
                   
                 tcacttcaccatgggtggtcttggcccaacagcaaga 
                   
                 LALDHVEEVEVVLA 
                   
               
               
                   
                   
                 caatggggattggctcttgaccacgtcgaagaagtgg 
                   
                 NSSIVRASNTQNQEV 
                   
               
               
                   
                   
                 aagttgttttggctaattcctctattgtcagagcatcaaa 
                   
                 FFAVKGAAASFGIVT 
                   
               
               
                   
                   
                 cactcaaaatcaagaagttttctttgctgttaagggtgct 
                   
                 EFKVRTQPAPGLAV 
                   
               
               
                   
                   
                 gccgcctccttcggtatcgtcactgaatttaaagtcaga 
                   
                 QYSYTFNLGSSAEK 
                   
               
               
                   
                   
                 actcaaccagccccaggattagctgttcaatactcttat 
                   
                 AQFVKDWQSFISAK 
                   
               
               
                   
                   
                 acgtttaacttgggtagctcagctgagaaggctcaatt 
                   
                 NLTRQFYTNMVIFD 
                   
               
               
                   
                   
                 cgtcaaggactggcaatctttcatttctgctaagaattta 
                   
                 GNIILEGLFFGSKEQ 
                   
               
               
                   
                   
                 accagacagttttacacaaacatggtaattttcgatggt 
                   
                 YEALGLEDRFVPKN 
                   
               
               
                   
                   
                 aacataatcctagaaggcctatttttcggttccaaagaa 
                   
                 PGNILVLTDWLGMV 
                   
               
               
                   
                   
                 caatacgaagctttgggtttggaggaccgttttgttcca 
                   
                 GHALEDTILRLVGN 
                   
               
               
                   
                   
                 aagaatcctggtaatatcttagtcttgacagattggcta 
                   
                 TPTWFYAKSLGFTP 
                   
               
               
                   
                   
                 ggtatggttggccatgcattggaggacactattttgag 
                   
                 DTLIPSSGIDEFFDYI 
                   
               
               
                   
                   
                 attggtcggtaacacccccacatggttctacgcaaaga 
                   
                 ENNKAGTLTWFVTL 
                   
               
               
                   
                   
                 gcttgggtttcactccagacacgttaatacctagttcag 
                   
                 SLEGGAINDVPKDA 
                   
               
               
                   
                   
                 gtattgatgaatttttcgattatatagaaaacaacaaggc 
                   
                 TAYGHRDVLFWVQI 
                   
               
               
                   
                   
                 tggtaccctgacgtggttcgttactttgagtttagaaggt 
                   
                 FMASPTGPVSSTTYD 
                   
               
               
                   
                   
                 ggtgccattaacgatgtgccaaaggatgctactgctta 
                   
                 FADGLYNVLTKAVP 
                   
               
               
                   
                   
                 tggtcacagagatgtgcttttttgggttcagatcttcatg 
                   
                 ESEGHAYLGCPDPK 
                   
               
               
                   
                   
                 gcttctccaacaggtcctgtctcttccactacctacgac 
                   
                 MADAQQKYWRQNL 
                   
               
               
                   
                   
                 ttcgccgatggtttgtataatgtcttgaccaaagccgttc 
                   
                 PRLEELKATLDPKDT 
                   
               
               
                   
                   
                 cagaatccgaaggacatgcttacctgggttgtccaga 
                   
                 FHNPQGILPA 
                   
               
               
                   
                   
                 cccaaagatggctgacgctcaacaaaagtactggag 
                   
                   
                   
               
               
                   
                   
                 gcaaaacttacctagacttgaagaattgaaagcaacct 
                   
                   
                   
               
               
                   
                   
                 tagatccaaaagatacttttcacaatccacaaggtatctt 
                   
                   
                   
               
               
                   
                   
                 gccagca 
                   
                   
                   
               
               
                   
               
               
                 t807859 
                 Library 
                 atgacaaccaagtgtaggcatgcagtggaggctactg 
                 43 
                 MTTKCRHAVEATAL 
                 113 
               
               
                   
                   
                 ctctaggcacgatggtagcctacctgattagaaataac 
                   
                 GTMVAYLIRNNPRR 
                   
               
               
                   
                   
                 ccacgtagacctagttcaactaacgctaatgttttggac 
                   
                 PSSTNANVLDTGLG 
                   
               
               
                   
                   
                 acaggtttgggtggtgccgatggtgctgttgtcgtcga 
                   
                 GADGAVVVDMKHF 
                   
               
               
                   
                   
                 tatgaaacactttactcagttctccatggacgatgaaac 
                   
                 TQFSMDDETYEAVI 
                   
               
               
                   
                   
                 ctatgaagctgttatcggaccaggtaccactttaaacg 
                   
                 GPGTTLNDVDIELY 
                   
               
               
                   
                   
                 acgttgatatagaattgtacaacaacggtaagagagct 
                   
                 NNGKRAMAHGVCP 
                   
               
               
                   
                   
                 atggcacacggtgtctgccccaccattaagactggtg 
                   
                 TIKTGGHFTIGGLGP 
                   
               
               
                   
                   
                 gtcatttcaccatcggcggacttggtccaactgccaga 
                   
                 TARQWGLALDHVE 
                   
               
               
                   
                   
                 caatggggtttggccttagatcacgttgaagaggttga 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 agtcgtgttggctaattcgtctattgtaagagctagcaa 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 cacacaaaaccaagacgttttctttgcagttaaaggtg 
                   
                 AANFGIVTEFKVRTE 
                   
               
               
                   
                   
                 ctgccgctaatttcggaatcgtcactgaatttaaggtca 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 gaaccgaaccagctcctggtttggctgttcaatattcct 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 acactttcaatttaggttctactgctgaaaaggctcaatt 
                   
                 QSFISAKNLTRQFYN 
                   
               
               
                   
                   
                 cgtgaaagattggcaatcttttatctctgctaagaatcta 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 acgagacagttctacaacaacatggttatttttgacggt 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 gatatcattcttgagggtttgtttttcggttccaaggaac 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 aatatgacgccttgggcttggaagatcatttcgctccaa 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 agaatccaggtaacatcttagtcctgacagactggcta 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 ggtatggttggacacgccttggaagacaccattttgaa 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 gcttgttggtaacaccccaacctggttctacgccaaat 
                   
                 EFFEYIANHTAGTPA 
                   
               
               
                   
                   
                 cgttgggttttagacaagatacattaattccatcagctg 
                   
                 WFVTLSLEGGAIND 
                   
               
               
                   
                   
                 gtatagacgaatttttcgaatacatagctaaccacactg 
                   
                 VAEDATAYAHRDV 
                   
               
               
                   
                   
                 ctggtactcccgcatggttcgtcacacttagtttggaag 
                   
                 LFWVQLFMVNPLGP 
                   
               
               
                   
                   
                 gtggtgctattaatgatgtcgcagaagatgccacggc 
                   
                 ISDTTYEFTDGLYDV 
                   
               
               
                   
                   
                 atatgctcaccgtgacgtcttgttctgggtacaactgttt 
                   
                 LARAVPESVGHAYL 
                   
               
               
                   
                   
                 atggtgaaccctttgggtcccatctctgacacgacttac 
                   
                 GCPDPRMEDAQQK 
                   
               
               
                   
                   
                 gaatttactgacggtttatatgatgttttggcaagagctg 
                   
                 YWRTNLPRLQELKE 
                   
               
               
                   
                   
                 ttccagagtccgtgggccatgcttacttaggttgtccag 
                   
                 ELDPKNTFHHPQGV 
                   
               
               
                   
                   
                 atcctagaatggaagatgctcagcaaaagtattggcgt 
                   
                 MPA 
                   
               
               
                   
                   
                 accaacttgccaaggttgcaagagttgaaagaagaac 
                   
                   
                   
               
               
                   
                   
                 tagacccaaagaacactttccaccatccacaaggtgtt 
                   
                   
                   
               
               
                   
                   
                 atgccagcc 
                   
                   
                   
               
               
                   
               
               
                 t807860 
                 Library 
                 atgggtcatggacaatccactcccggtgagaactgttt 
                 44 
                 MGHGQSTPGENCLN 
                 114 
               
               
                   
                   
                 aaatgcaatatgcggtaacagaacggactgtgtatcat 
                   
                 AICGNRTDCVSYPL 
                   
               
               
                   
                   
                 acccattggatcctctattccacatcgcttgggcccgtc 
                   
                 DPLFHIAWARPYNL 
                   
               
               
                   
                   
                 catataaccttcaggtgcctgttacaccagctgctgtcc 
                   
                 QVPVTPAAVLRPDN 
                   
               
               
                   
                   
                 tgaggccagacaatgctcaagatgttgctgatgccgtt 
                   
                 AQDVADAVKCANE 
                   
               
               
                   
                   
                 aaatgtgccaacgaaaatggcattaaggtccaagcta 
                   
                 NGIKVQARSGGHSY 
                   
               
               
                   
                   
                 gatctggtggtcactcgtacggtaactttggtttgggag 
                   
                 GNFGLGGDDGALVL 
                   
               
               
                   
                   
                 gtgacgacggtgcattggttttggatctagtcaatttac 
                   
                 DLVNLQGFTMDEEN 
                   
               
               
                   
                   
                 aaggtttcactatggacgaagaaaactggcatgctgct 
                   
                 WHAAVGAGIRLGKL 
                   
               
               
                   
                   
                 gttggcgcaggtattagattgggtaagctggatgaaca 
                   
                 DEHLHKNGGRAMA 
                   
               
               
                   
                   
                 cttgcataaaaatggtggtagagctatggctcacggta 
                   
                 HGTCPGVGIGGHATI 
                   
               
               
                   
                   
                 cctgtccaggcgtaggtatcggtggtcacgccaccat 
                   
                 GGLGPMSRMWGSA 
                   
               
               
                   
                   
                 cggaggtttaggtccaatgagcagaatgtggggttct 
                   
                 LDHVVEVEVVTADG 
                   
               
               
                   
                   
                 gccttggatcatgtcgtcgaagttgaagttgttactgct 
                   
                 SIQRANETENSDLFW 
                   
               
               
                   
                   
                 gacggctctattcaacgtgctaacgaaacagaaaata 
                   
                 ALRGAGASFGIITEF 
                   
               
               
                   
                   
                 gtgatttattttgggccttgagaggtgctggtgcttcctt 
                   
                 VVRTHPEPGDVIEYT 
                   
               
               
                   
                   
                 cggtataatcactgagtttgtagttagaacccacccag 
                   
                 YSLTFGSQAEMAPIY 
                   
               
               
                   
                   
                 aaccaggagatgtaattgaatatacctactctctgacct 
                   
                 QEWQEFIGDPDLDR 
                   
               
               
                   
                   
                 tcggttcacaagcagaaatggctcctatttaccaagaa 
                   
                 RFSSQFIAQPLGAIIT 
                   
               
               
                   
                   
                 tggcaggaatttattggtgacccagacttggatagaag 
                   
                 GTFYGTEDEYRESGI 
                   
               
               
                   
                   
                 gttctcctctcaattcattgctcaaccattgggtgctatc 
                   
                 PDKIPGGGSDGVGIL 
                   
               
               
                   
                   
                 atcactggaaccttctacggtactgaagacgaatatag 
                   
                 VTDWLGSLGHQAER 
                   
               
               
                   
                   
                 agagagtggtatccccgataagataccaggaggtggt 
                   
                 SGLAISDLPSPFYSKS 
                   
               
               
                   
                   
                 agtgatggtgttggtattttagttacagactggttgggtt 
                   
                 LAFRKEDLLPKEGIT 
                   
               
               
                   
                   
                 ctctaggccaccaagccgaaagatccggtcttgctatt 
                   
                 ELFQYLNDADHGTL 
                   
               
               
                   
                   
                 tcggatcttccaagccctttttattctaagtcgttggcctt 
                   
                 IWVVIFNSEGGAMG 
                   
               
               
                   
                   
                 cagaaaggaagacttgttgccaaaagagggtataact 
                   
                 DTAANATAYPHRDK 
                   
               
               
                   
                   
                 gagttattccagtacttgaacgacgctgaccatggtac 
                   
                 TMMYQSYVIGIPEVS 
                   
               
               
                   
                   
                 attgatctgggtcgtcatctttaactccgaaggaggtgc 
                   
                 GTARRFLEGVHAKI 
                   
               
               
                   
                   
                 aatgggtgatactgccgcaaacgctacagcctaccca 
                   
                 QDAAPGANSTYAGY 
                   
               
               
                   
                   
                 catcgtgataagaccatgatgtatcaatcctacgtcatt 
                   
                 IDTELGRAEAQEVY 
                   
               
               
                   
                   
                 ggtatccccgaagtgagtggcaccgcaagaagattc 
                   
                 WGSQLPQLRKIKKD 
                   
               
               
                   
                   
                 ctggagggtgttcacgctaagattcaagatgctgctcc 
                   
                 WDPKDRFSNPQSVQ 
                   
               
               
                   
                   
                 tggtgctaattcaacgtatgctggttacatcgatacgga 
                   
                 AAR 
                   
               
               
                   
                   
                 attaggcagagctgaagctcaagaagtgtactggggt 
                   
                   
                   
               
               
                   
                   
                 agccagttgcctcaattgagaaagatcaaaaaggact 
                   
                   
                   
               
               
                   
                   
                 gggacccaaaggacaggttttcaaacccacaatctgt 
                   
                   
                   
               
               
                   
                   
                 ccaagccgccaga 
                   
                   
                   
               
               
                   
               
               
                 t807861 
                 Library 
                 atgcgtgtcgttggaaagatgggtgctttgcaaagcac 
                 45 
                 MRVVGKMGALQST 
                 115 
               
               
                   
                   
                 tctggagaaatctatcaaggccgcattagctggtgacg 
                   
                 LEKSIKAALAGDDD 
                   
               
               
                   
                   
                 atgatctatacgctgtgcccggtaaaccattttatcagat 
                   
                 LYAVPGKPFYQIQH 
                   
               
               
                   
                   
                 acaacatgtcaagccttacaacttgtcgattccaatcga 
                   
                 VKPYNLSIPIEPAAIT 
                   
               
               
                   
                   
                 accagccgctattacctatcctaagacaactgctcaag 
                   
                 YPKTTAQVAAIIKCA 
                   
               
               
                   
                   
                 tagccgcaattatcaagtgcgctgttgctgctaatttga 
                   
                 VAANLKVQARSGG 
                   
               
               
                   
                   
                 aggtccaagccagatcaggtggccactcctacgctaa 
                   
                 HSYANYCIGGVSGA 
                   
               
               
                   
                   
                 ctactgtattggtggtgtttctggtgctgttgttatcgacc 
                   
                 VVIDLKHFQRFSMD 
                   
               
               
                   
                   
                 ttaaacacttccaaagattcagtatggatagaaccacgt 
                   
                 RTTWQAAVGAGTL 
                   
               
               
                   
                   
                 ggcaagcagccgtcggtgctggcactttattgggtaat 
                   
                 LGNLTKRMHEAGN 
                   
               
               
                   
                   
                 ttgaccaagaggatgcatgaagctggtaacagagcc 
                   
                 RAMAHGTCPQVGIG 
                   
               
               
                   
                   
                 atggctcacggtacttgtccacaagtgggaattggtgg 
                   
                 GHATIGGLGPSSRL 
                   
               
               
                   
                   
                 tcacgcaaccataggtggccttggtccatcttcaagatt 
                   
                 WGTALDHVEEVEIV 
                   
               
               
                   
                   
                 gtggggtacggctttagaccatgttgaagaagtcgaa 
                   
                 LADSTIKRCSATQNP 
                   
               
               
                   
                   
                 atagtcttggctgattccacaattaagagatgttctgcta 
                   
                 DIFWAVKGAGASFG 
                   
               
               
                   
                   
                 ctcagaatccagacatcttttgggccgttaagggagct 
                   
                 VVTEFKLRTEPEPSE 
                   
               
               
                   
                   
                 ggtgcatccttcggtgttgtgactgaatttaaattaagaa 
                   
                 AVHFSYSFTVGSYA 
                   
               
               
                   
                   
                 ccgagcccgaaccatctgaagctgtacatttctcttatt 
                   
                 SLAAVFKSWQSFVA 
                   
               
               
                   
                   
                 cgttcactgttggttcctacgcaagcttggctgctgttttt 
                   
                 DPGLTRKFSSEVIITE 
                   
               
               
                   
                   
                 aaatcatggcaatctttcgtcgctgacccaggtcttact 
                   
                 IGMIISGTYFGSQAE 
                   
               
               
                   
                   
                 cgtaagttctcctctgaagtcatcattacagagatcggt 
                   
                 YDALDMKSQLRGDS 
                   
               
               
                   
                   
                 atgattatatcaggcacttattttggtagtcaagctgaat 
                   
                 VAKIIVFKDWLGLL 
                   
               
               
                   
                   
                 acgatgccctagatatgaagtctcaattgagaggtgac 
                   
                 GHWAEDVGLRIAGG 
                   
               
               
                   
                   
                 agtgttgctaagatcattgtttttaaggactggttaggatt 
                   
                 LPAPLYAKTLTFNG 
                   
               
               
                   
                   
                 gttgggtcactgggccgaagatgtgggcctaagaatt 
                   
                 ANLIPDEVIDKLFAY 
                   
               
               
                   
                   
                 gccggtggtttacctgcccctttgtacgctaaaaccttg 
                   
                 LDKVEKGALVWFVI 
                   
               
               
                   
                   
                 accttcaacggtgccaacctgatcccagatgaagtcat 
                   
                 FDLAGGAVNDIAQD 
                   
               
               
                   
                   
                 cgataaattgttcgcctacctggacaaggttgaaaagg 
                   
                 ATSYAHRDALFYLQ 
                   
               
               
                   
                   
                 gagctttggtatggttcgtcatttttgacctggctggag 
                   
                 SYAVGLGNVSQTTK 
                   
               
               
                   
                   
                 gtgccgttaatgacatagctcaagatgctacatcctatg 
                   
                 DFLTGINTTITNGMP 
                   
               
               
                   
                   
                 ctcatcgtgatgccttgttctacttgcagtcatatgcagt 
                   
                 EGGDFGAYPGYVDL 
                   
               
               
                   
                   
                 gggtttaggtaacgtttcacaaacaactaaggattttctt 
                   
                 ELPNGPHAYWRTNL 
                   
               
               
                   
                   
                 accggtataaacacgactattaccaacggtatgccag 
                   
                 PRLEQIKALVDPND 
                   
               
               
                   
                   
                 aaggtggtgacttcggtgcttacccaggctacgttgac 
                   
                 VFHNPQSYLCILFLL 
                   
               
               
                   
                   
                 ttggaattaccaaatggtccacacgcttactggagaac 
                   
                 NLLNRALAWAPVGT 
                   
               
               
                   
                   
                 caaccttccaaggttggaacaaatcaaagccctggta 
                   
                 VQPFQVLRYSIDTGP 
                   
               
               
                   
                   
                 gatcctaatgatgtcttccacaacccacaatcttatttgt 
                   
                 LVLL 
                   
               
               
                   
                   
                 gcatcctatttttgctaaacttgctaaacagagctttggc 
                   
                   
                   
               
               
                   
                   
                 ttgggctccagttggtactgtccagccattccaagtctt 
                   
                   
                   
               
               
                   
                   
                 aaggtactccattgacacaggtcctcttgtgcttttg 
                   
                   
                   
               
               
                   
               
               
                 t807863 
                 Library 
                 atgggtcagggctcgagcggtgtgcaatctaacccct 
                 46 
                 MGQGSSGVQSNPLE 
                 116 
               
               
                   
                   
                 tagaagattgtttgaaggtagctacaagtccactaggtt 
                   
                 DCLKVATSPLGSYA 
                   
               
               
                   
                   
                 catacgccttccatgacaaattgctgtttcaacttaccg 
                   
                 FHDKLLFQLTDVKP 
                   
               
               
                   
                   
                 atgttaagccttataatttagactacccagtcaacccaat 
                   
                 YNLDYPVNPIAVTY 
                   
               
               
                   
                   
                 cgctgttacgtatccaggttccactaaagaggttgcac 
                   
                 PGSTKEVAQIIKCAT 
                   
               
               
                   
                   
                 aaattataaagtgcgctaccacttacgataagaaggtc 
                   
                 TYDKKVQARSGGHS 
                   
               
               
                   
                   
                 caagccagaagcggaggtcactcttacgctaatttcg 
                   
                 YANFALGDGDGAIV 
                   
               
               
                   
                   
                 ctttgggtgacggtgacggtgcaattgttatcgatatgc 
                   
                 IDMQKFKQFSMDTS 
                   
               
               
                   
                   
                 aaaaatttaagcaattctccatggacacttctacctggc 
                   
                 TWQATIGPGTLLGD 
                   
               
               
                   
                   
                 aggctacaattggtcctggtactttgttgggtgatgtctc 
                   
                 VSKRLHENGNRVIP 
                   
               
               
                   
                   
                 caagcgtttacacgaaaacggtaacagggtaatccca 
                   
                 HGTSPQIGFGGHGTI 
                   
               
               
                   
                   
                 catggaacctctccacaaataggtttcggaggccacg 
                   
                 GGLGPLSRMYGLTL 
                   
               
               
                   
                   
                 gtactattggtggtctgggccctttgtctcgtatgtacgg 
                   
                 DSIEEVEAVLANGQI 
                   
               
               
                   
                   
                 tttaaccttggactccatcgaagaagttgaagccgtctt 
                   
                 VRASKTQNEDLFFAI 
                   
               
               
                   
                   
                 ggctaacggtcaaattgttagagctagtaaaactcaaa 
                   
                 RGAAASVAVVTEFK 
                   
               
               
                   
                   
                 atgaagatctattttttgctattagaggagccgccgcttc 
                   
                 VRTYPEPSSSVLYSY 
                   
               
               
                   
                   
                 agtcgcagttgtcacagaatttaaggttagaacctatcc 
                   
                 TLQGGSVASRANAF 
                   
               
               
                   
                   
                 agagccctctagttctgtgttatattcttacactttacaag 
                   
                 KQWQKLTTDPSVSR 
                   
               
               
                   
                   
                 gtggttcagttgcttccagagctaacgctttcaagcagt 
                   
                 KFASTFVLSEAITVV 
                   
               
               
                   
                   
                 ggcaaaaattgacgacagatccatcggtcagcagaa 
                   
                 TGTFFGTQAEFDSLD 
                   
               
               
                   
                   
                 agttcgcttctactttcgttctatccgaagccataaccgt 
                   
                 ITSRLPADMISNNTE 
                   
               
               
                   
                   
                 cgtcacgggtactttcttcggtactcaagctgagtttgat 
                   
                 VKNWLGVVGHWGE 
                   
               
               
                   
                   
                 tccttggacatcacctctaggttgcctgccgacatgatc 
                   
                 SLALRAGGGIPAHFY 
                   
               
               
                   
                   
                 tccaataatacagaagttaagaactggttgggtgtcgtt 
                   
                 SKSLGFKKDEIMDD 
                   
               
               
                   
                   
                 ggccattggggtgaatcattggctttgagagccggtg 
                   
                 ATVDKLFNYIDKAD 
                   
               
               
                   
                   
                 gtggtattccagcacacttttactccaagtctttgggtttc 
                   
                 KGGAVWFVIWDLE 
                   
               
               
                   
                   
                 aaaaaggatgagatcatggatgatgctactgtggaca 
                   
                 GGAISDVPTTETSYG 
                   
               
               
                   
                   
                 agctattcaattatattgacaaagctgataaaggaggtg 
                   
                 HRDAIFFQQSYAINL 
                   
               
               
                   
                   
                 ctgtttggttcgttatttgggaccttgaaggaggtgctat 
                   
                 LGRVKDDTHEFLNR 
                   
               
               
                   
                   
                 ctctgatgttccaaccactgaaacttcttacggtcatag 
                   
                 VNSVIMESNPGGYW 
                   
               
               
                   
                   
                 agatgcaatctttttccaacagtcttatgcaattaacttat 
                   
                 GAYPGYVDTALGNS 
                   
               
               
                   
                   
                 tgggtagagttaaggacgacacccacgaatttttgaac 
                   
                 SAKAYWGINSERLQ 
                   
               
               
                   
                   
                 agagttaatagtgtaattatggaatctaacccaggtggt 
                   
                 TIKSWVDAGDVFHN 
                   
               
               
                   
                   
                 tactggggtgcctacccaggttatgtcgatactgctcta 
                   
                 PQSVRPK 
                   
               
               
                   
                   
                 ggtaattccagcgctaaggcctactggggtatcaaca 
                   
                   
                   
               
               
                   
                   
                 gcgaaagattacaaaccataaaaagttgggtagacgc 
                   
                   
                   
               
               
                   
                   
                 tggtgatgtgtttcacaacccacaatcagttagaccca 
                   
                   
                   
               
               
                   
                   
                 ag 
                   
                   
                   
               
               
                   
               
               
                 t807866 
                 Library 
                 atgcagccttttacaagccttactaggtcccccttccgtt 
                 47 
                 MQPFTSLTRSPFRSA 
                 117 
               
               
                   
                   
                 cagcccacgttatcagttgtccagtcgctttggacaatc 
                   
                 HVISCPVALDNPPSV 
                   
               
               
                   
                   
                 caccatcggtaccaattataatgggacaaaagccttcc 
                   
                 PIIMGQKPSSPLATC 
                   
               
               
                   
                   
                 tctccattagctacctgcttggataaagtttgtaacggta 
                   
                 LDKVCNGRSSCVGY 
                   
               
               
                   
                   
                 gatctagttgtgtcggttacccaaacgaccccctattcc 
                   
                 PNDPLFQINWVKPY 
                   
               
               
                   
                   
                 aaatcaattgggttaagccatataacttggatattcctgt 
                   
                 NLDIPVQPIAVTRPS 
                   
               
               
                   
                   
                 ccaaccaattgcagtgactagaccatctaccgctgag 
                   
                 TAEDVAGFVKCAAE 
                   
               
               
                   
                   
                 gatgttgccggttttgttaagtgtgctgctgaaaacaat 
                   
                 NNVKVQAKSGGHS 
                   
               
               
                   
                   
                 gtcaaagtccaagcaaagtctggcggtcattcctacg 
                   
                 YGNFAIGGTDGALVI 
                   
               
               
                   
                   
                 gtaacttcgctatcggtggtactgacggtgccttagtta 
                   
                 DLVNFQNFSMDTNT 
                   
               
               
                   
                   
                 ttgatctggtgaattttcaaaacttcagcatggatacaaa 
                   
                 WQATFGGGHKLHE 
                   
               
               
                   
                   
                 cacctggcaggctacgttcggtggaggccacaagttg 
                   
                 VTQKLHDNGKRAIA 
                   
               
               
                   
                   
                 catgaagttactcaaaaactacacgacaatggtaaga 
                   
                 HGTCPGVGIGGHATI 
                   
               
               
                   
                   
                 gagctatcgcccacggtacctgtccaggtgttggtata 
                   
                 GGLGPSSRMWGSCL 
                   
               
               
                   
                   
                 ggtggacatgctactattggtggtttgggtccatcttctc 
                   
                 DHVVEVEVVTADG 
                   
               
               
                   
                   
                 gtatgtggggctcctgcttggatcacgtagttgaagtc 
                   
                 KIQRANDKQNSDLF 
                   
               
               
                   
                   
                 gaagtcgttaccgcagacggtaagatccaaagagcta 
                   
                 FALKGAGAGFGVIT 
                   
               
               
                   
                   
                 acgataagcaaaattccgacttgttctttgccttaaaag 
                   
                 EFVMRTHPEPGDVV 
                   
               
               
                   
                   
                 gtgcaggagctggttttggtgtcattactgagttcgtga 
                   
                 QYSYAITFAKHRDL 
                   
               
               
                   
                   
                 tgagaacccatccagaacctggtgacgttgttcaatatt 
                   
                 VPVFKQWQELIFDPT 
                   
               
               
                   
                   
                 cttacgctatcacttttgctaaacacagagacttggttcc 
                   
                 LDRRFSSEFVMQEL 
                   
               
               
                   
                   
                 tgtattcaagcaatggcaagaactgattttcgatccaac 
                   
                 GVAITATFYGTEDEF 
                   
               
               
                   
                   
                 acttgatagacgtttctcatctgaatttgtcatgcaagaa 
                   
                 KKTGIPDRIPKGKVS 
                   
               
               
                   
                   
                 ttaggtgtcgctataacggccactttttacggcacgga 
                   
                 VVINNWLGDVAQK 
                   
               
               
                   
                   
                 ggatgaatttaagaagactggtattccagacagaatcc 
                   
                 AQDAALWLSDIQSA 
                   
               
               
                   
                   
                 ccaaaggtaaagtttccgtcgttataaacaattggttgg 
                   
                 FTSKSLAFTHNDLIS 
                   
               
               
                   
                   
                 gtgatgtcgcacagaaggctcaagatgcagccttgtg 
                   
                 EDGIQTMMDYVDSV 
                   
               
               
                   
                   
                 gcttagtgatattcaatcagctttcacctctaagtccttg 
                   
                 DRGTLIWFLILDSTG 
                   
               
               
                   
                   
                 gctttcacccataacgacctaatctcggaagacggtat 
                   
                 GAINDVPMNATAYR 
                   
               
               
                   
                   
                 ccaaactatgatggactatgttgattcagtcgatagag 
                   
                 HRDKVMFFQGYGV 
                   
               
               
                   
                   
                 gcacattaatttggttcttgattttggattctactggagga 
                   
                 GIPTLSGKTKDFMSG 
                   
               
               
                   
                   
                 gctattaatgacgttccaatgaacgctacagcctacag 
                   
                 VADKIRKASPNELST 
                   
               
               
                   
                   
                 acacagggacaaagtgatgttcttccaaggttacggtg 
                   
                 YAGYVDPTLDNAQE 
                   
               
               
                   
                   
                 ttggtataccaaccctttctggtaagaccaaggattttat 
                   
                 RYWGPNLPALERIK 
                   
               
               
                   
                   
                 gtccggtgttgctgataagatccgtaaggcctctccta 
                   
                 ATWDPKDLFSNPQS 
                   
               
               
                   
                   
                 acgaattgagcacttacgctggatacgtagacccaact 
                   
                 VRPNASAKDVEPAA 
                   
               
               
                   
                   
                 ttggacaatgctcaagaaagatattggggtccaaactt 
                   
                 SGGSNNSGSKGGDS 
                   
               
               
                   
                   
                 accagccctagaaagaataaaagctacctgggatcct 
                   
                   
                   
               
               
                   
                   
                 aaggacttattctcaaacccacagtcagtgaggccaa 
                   
                   
                   
               
               
                   
                   
                 acgcttccgccaaggatgtcgaacctgccgcatctgg 
                   
                   
                   
               
               
                   
                   
                 tggttccaataattcgggttctaaaggtggagacagt 
                   
                   
                   
               
               
                   
               
               
                 t807869 
                 Library 
                 atgggatccggtcatagttctggcttggccacttgctta 
                 48 
                 MGSGHSSGLATCLD 
                 118 
               
               
                   
                   
                 gatgcagtgtgtaatggtcgtcacgcttgtgtagcttac 
                   
                 AVCNGRHACVAYP 
                   
               
               
                   
                   
                 cctgaccacctactgtatcaagcctcttgggtcgatag 
                   
                 DHLLYQASWVDRY 
                   
               
               
                   
                   
                 atacaaccttgacatcccagttcatcccatagctgttac 
                   
                 NLDIPVHPIAVTRPS 
                   
               
               
                   
                   
                 caggccatcaaacgcagacgatgtcagcggttttgtta 
                   
                 NADDVSGFVKCAA 
                   
               
               
                   
                   
                 aatgtgctgccgctaataacgtcagagttcaggctaag 
                   
                 ANNVRVQAKSGGH 
                   
               
               
                   
                   
                 tctggtggtcactcgtatgctaattacggcttgggtggt 
                   
                 SYANYGLGGEDGEL 
                   
               
               
                   
                   
                 gaggatggtgaattagttattgacttgagacatttgcaa 
                   
                 VIDLRHLQHFSMDT 
                   
               
               
                   
                   
                 cacttctcaatggatacgaacacttggcaagctaccatt 
                   
                 NTWQATIGAGHRL 
                   
               
               
                   
                   
                 ggtgccggtcacagattatgggacgttacacataagtt 
                   
                 WDVTHKLHENGKR 
                   
               
               
                   
                   
                 gcacgaaaacggtaagagagcagtcagccacggaa 
                   
                 AVSHGTCPGVGIGG 
                   
               
               
                   
                   
                 cttgcccaggtgttggtattggcggtcatgccaccatc 
                   
                 HATIGGLGPSSRMW 
                   
               
               
                   
                   
                 ggtggtctaggtccatcctctcgtatgtggggatcgtgt 
                   
                 GSCLDHVVEVEVVT 
                   
               
               
                   
                   
                 ttggatcacgtggtcgaagttgaagttgtgactgctga 
                   
                 ADGSIRRASERENA 
                   
               
               
                   
                   
                 cggttctataagaagagcttccgaaagagaaaacgct 
                   
                 DLFFALKGAGAGFG 
                   
               
               
                   
                   
                 gatttgttctttgctttaaaaggtgccggtgctggtttcg 
                   
                 VITEFVMKTHPEPGS 
                   
               
               
                   
                   
                 gtgtgatcaccgaatttgtaatgaagactcaccctgaa 
                   
                 VVRYTYSVNFGRHA 
                   
               
               
                   
                   
                 ccaggatctgttgtcatgaggtacacatactccgttaat 
                   
                 DMVDVFDQWQALIS 
                   
               
               
                   
                   
                 ttcggtagacatgcagacatggtcgacgtattcgatca 
                   
                 DPGLDRRFGSEIIMH 
                   
               
               
                   
                   
                 atggcaagctttgatttctgatccaggtctggatagaag 
                   
                 AFGLVISATFHGTRD 
                   
               
               
                   
                   
                 atttggaagtgaaattatcatgcacgcattcggcctagt 
                   
                 EYEASGIPDRIPRGN 
                   
               
               
                   
                   
                 catttccgctacgttccatggtaccagagatgagtatga 
                   
                 VSVLLDNWLGVVG 
                   
               
               
                   
                   
                 agcttctggtatcccagacagaatccctcgtggtaacg 
                   
                 NQAQDAGLWVSEV 
                   
               
               
                   
                   
                 tgtccgttttgttggacaattggttaggtgtcgttggtaat 
                   
                 RSSFTSRSLAFRRDQ 
                   
               
               
                   
                   
                 caggcccaagatgctggattgtgggtttctgaggttag 
                   
                 LLSRDDIVRMMDFL 
                   
               
               
                   
                   
                 atcgagtttcacttcacgttcattggcttttagaagggac 
                   
                 DRTDKGTLVWFLIF 
                   
               
               
                   
                   
                 caacttctatctcgtgatgatattgtcagaatgatggact 
                   
                 DVTGGAIGDVRTDA 
                   
               
               
                   
                   
                 ttttggacagaactgataagggtacgttagtctggttttt 
                   
                 TAYAHRDKIMFCQG 
                   
               
               
                   
                   
                 gattttcgacgtcacaggtggtgctattggcgacgtta 
                   
                 YAVGIPALTRKTRVF 
                   
               
               
                   
                   
                 gaactgacgcaaccgcctacgctcatagagataagat 
                   
                 MDGLISTIRETANST 
                   
               
               
                   
                   
                 catgttctgtcaaggttacgcagttggtataccagctctt 
                   
                 LTTYPGYVDPSLHD 
                   
               
               
                   
                   
                 accagaaaaactcgtgtcttcatggacggtttaatttcc 
                   
                 AQASYWGPNLPRLT 
                   
               
               
                   
                   
                 actatcagggaaaccgccaactctactctaaccaccta 
                   
                 EVKTKWDPQDVFH 
                   
               
               
                   
                   
                 tcccggatacgtcgatccaagtttgcacgacgctcaa 
                   
                 NPQSVRPSGKD 
                   
               
               
                   
                   
                 gcttcctactggggtcctaacttgccaagattaacaga 
                   
                   
                   
               
               
                   
                   
                 agttaagactaagtgggatccacaggatgtttttcacaa 
                   
                   
                   
               
               
                   
                   
                 cccacaatctgtaagaccatctggtaaagat 
                   
                   
                   
               
               
                   
               
               
                 t807873 
                 Library 
                 atgggtaacactacatcaatagctgccggccgtgact 
                 50 
                 MGNTTSIAAGRDCL 
                 120 
               
               
                   
                   
                 gcctattgagcgctgtgggtggaaatcacgcacatgtt 
                   
                 LSAVGGNHAHVAFQ 
                   
               
               
                   
                   
                 gcttttcaggatcaacttttataccaagctaccgccgtc 
                   
                 DQLLYQATAVEPYN 
                   
               
               
                   
                   
                 gaaccctataacttgaatatccctgtaactccagcagct 
                   
                 LNIPVTPAAVTYPQS 
                   
               
               
                   
                   
                 gttacgtacccacaaagtgctgatgaggttgccgctgt 
                   
                 ADEVAAVVKCAAD 
                   
               
               
                   
                   
                 cgttaaatgtgccgctgactacggttataaggttcaag 
                   
                 YGYKVQARSGGHSF 
                   
               
               
                   
                   
                 ctaggtccggtggtcactcgttcggtaactacggtttg 
                   
                 GNYGLGGEDGAIVV 
                   
               
               
                   
                   
                 ggaggtgaagacggtgctattgtcgttgatatgaagca 
                   
                 DMKHFDQFSMDEST 
                   
               
               
                   
                   
                 tttcgatcagttttccatggacgaatctacctatactgca 
                   
                 YTATIGPGITLGDLD 
                   
               
               
                   
                   
                 acgatcggtccaggcattactttaggtgatctggatac 
                   
                 TALYNAGHRAMAH 
                   
               
               
                   
                   
                 cgccttgtacaacgctggtcacagagctatggctcatg 
                   
                 GICPTIRTGGHLTIGG 
                   
               
               
                   
                   
                 gtatctgtccaacaattagaactggtggtcaccttacca 
                   
                 LGPTARQWGLALDH 
                   
               
               
                   
                   
                 ttggtggattaggtcctacagctagacaatggggcttg 
                   
                 VEEVEVVLANSSIVR 
                   
               
               
                   
                   
                 gccctggaccacgttgaagaagtggaagtcgtcttgg 
                   
                 ASDTQNQEILFAVK 
                   
               
               
                   
                   
                 ctaactcgtctatagttagagcatctgacacccaaaatc 
                   
                 GAAASFGIVTEFKVR 
                   
               
               
                   
                   
                 aagaaatcttgttcgctgtaaaaggtgctgctgcctcat 
                   
                 TEEAPGLAVQYSFTF 
                   
               
               
                   
                   
                 tcggtattgtgactgaatttaaggttcgtactgaggaag 
                   
                 NLGTAAEKAKLVKD 
                   
               
               
                   
                   
                 ccccaggtttggccgtccaatattctttcacctttaattta 
                   
                 WQAFIAQEDLTWKF 
                   
               
               
                   
                   
                 ggtactgctgctgaaaaggcaaagctggttaaagact 
                   
                 YSNMNIIDGQIILEGI 
                   
               
               
                   
                   
                 ggcaagctttcatcgctcaggaggatcttacttggaag 
                   
                 YFGSKAEYDALGLE 
                   
               
               
                   
                   
                 ttctactctaacatgaacattattgatggtcaaatcatctt 
                   
                 EKFPTSEPGTVLVLT 
                   
               
               
                   
                   
                 ggaaggcatctactttggttctaaggccgaatatgacg 
                   
                 DWLGMVGHGLEDV 
                   
               
               
                   
                   
                 ctctaggtttggaggaaaaatttccaacctccgaacca 
                   
                 ILRLVGNAPTWFYA 
                   
               
               
                   
                   
                 ggaaccgtcttggtattgactgactggctaggcatggt 
                   
                 KSLGFAPRALIPDSAI 
                   
               
               
                   
                   
                 gggtcacggtttggaagatgttatattaagattggtcgg 
                   
                 DDFFEYIHKNNPGT 
                   
               
               
                   
                   
                 taatgccccaacttggttctacgccaagtcccttggattt 
                   
                 VSWFVTLSLEGGAI 
                   
               
               
                   
                   
                 gcaccaagagcactaattcctgattccgcaattgatga 
                   
                 NKVPEDATAYGHRD 
                   
               
               
                   
                   
                 cttcttcgaatacatccataagaacaaccccggtaccg 
                   
                 VLFWVQIFMINPLGP 
                   
               
               
                   
                   
                 tttcttggttcgttactttgagtttagagggtggtgctata 
                   
                 VSQTIYDFADGLYD 
                   
               
               
                   
                   
                 aataaggtcccagaagatgctaccgcttatggtcatag 
                   
                 VLAKAVPESAGHAY 
                   
               
               
                   
                   
                 agatgttctattctgggtacaaattttcatgatcaatccttt 
                   
                 LGCPDPRMPNAQQA 
                   
               
               
                   
                   
                 gggtccagtctcacaaactatttacgactttgcagacg 
                   
                 YWRNNLPRLEELKG 
                   
               
               
                   
                   
                 gattgtacgatgttttagccaaagctgttccagaaagc 
                   
                 DLDPKDIFHNPQGV 
                   
               
               
                   
                   
                 gctggtcatgcttacttgggttgtcccgacccaagaat 
                   
                 MVVS 
                   
               
               
                   
                   
                 gccaaacgctcaacaagcttactggaggaacaatttg 
                   
                   
                   
               
               
                   
                   
                 cctagattagaagaacttaagggtgatttggacccaaa 
                   
                   
                   
               
               
                   
                   
                 agatatattccacaacccacaaggtgtcatggttgtttc 
                   
                   
                   
               
               
                   
                   
                 c 
                   
                   
                   
               
               
                   
               
               
                 t807878 
                 Library 
                 atgggtcaatcccccagttcacttttagccacttgccta 
                 51 
                 MGQSPSSLLATCLN 
                 121 
               
               
                   
                   
                 aataccgtttgtgacggcagaacagattgtgtagcata 
                   
                 TVCDGRTDCVAYPN 
                   
               
               
                   
                   
                 ccctaacaacccattgtatcagatcagctgggtcaacc 
                   
                 NPLYQISWVNRYNL 
                   
               
               
                   
                   
                 gttacaatctggatttgccagttactcctattgctgtcac 
                   
                 DLPVTPIAVTRPQTV 
                   
               
               
                   
                   
                 cagaccacaaacggttcaagacgtgtctgcttttgttaa 
                   
                 QDVSAFVKCAATNN 
                   
               
               
                   
                   
                 atgtgctgccactaacaatataaaggtccaaccaaagt 
                   
                 IKVQPKSGGHSYAN 
                   
               
               
                   
                   
                 ctggtggacactcttacgctaactatggtggtgaagac 
                   
                 YGGEDGALVIDLLK 
                   
               
               
                   
                   
                 ggtgctttagttattgatttgttgaagttgcaagatttctc 
                   
                 LQDFSMDAKTWQA 
                   
               
               
                   
                   
                 catggacgccaaaacctggcaggctactatcggtggt 
                   
                 TIGGGTKLADVTKR 
                   
               
               
                   
                   
                 ggtacaaagttggctgatgtcaccaagagactgcatg 
                   
                 LHDNGKRAISHGTC 
                   
               
               
                   
                   
                 ataacggtaaaagggcaatttctcacggtacttgtcca 
                   
                 PGVGIGGHATIGGLG 
                   
               
               
                   
                   
                 ggcgttggtatcggtggtcatgctaccatcggtggctt 
                   
                 PTSRMWGSCLDHVV 
                   
               
               
                   
                   
                 gggacctacttcgagaatgtggggttcctgcttagacc 
                   
                 EAEVVTADGSIKRA 
                   
               
               
                   
                   
                 acgtcgtggaggctgaagttgtgactgccgatggtag 
                   
                 SETENRDLFFALKG 
                   
               
               
                   
                   
                 tattaagagagcctctgaaacagaaaatcgtgacttgtt 
                   
                 AGAGFGVVTKFVM 
                   
               
               
                   
                   
                 cttcgctcttaaaggtgcaggagcaggttttggtgttgt 
                   
                 KTHPEPGSMVQYSY 
                   
               
               
                   
                   
                 cacgaagtttgttatgaagacccacccagaaccaggt 
                   
                 SLSFGKHTDMVPVF 
                   
               
               
                   
                   
                 agcatggtacaatactcctattcactatctttcggtaaac 
                   
                 KQWQDLVSDPNLD 
                   
               
               
                   
                   
                 atactgatatggtaccagtttttaagcaatggcaagattt 
                   
                 RRFGTEFVAHELGAI 
                   
               
               
                   
                   
                 agtcagtgaccccaatttggacagaagattcggcact 
                   
                 ITATFYGTEAEWDA 
                   
               
               
                   
                   
                 gaatttgttgctcatgagttgggtgctattatcaccgcta 
                   
                 SGIPQRIPKGKISVIID 
                   
               
               
                   
                   
                 ctttctacggtacagaagctgaatgggatgctagcgg 
                   
                 DWLAVISQQAEDAA 
                   
               
               
                   
                   
                 catcccacaaagaatcccaaagggtaagatatccgtc 
                   
                 LYLSDIHSAFTVRSL 
                   
               
               
                   
                   
                 attattgatgattggctagccgttatttcccagcaagca 
                   
                 AFTAEETLSEQTITR 
                   
               
               
                   
                   
                 gaggacgctgccctatatttgtctgacattcactccgct 
                   
                 VMKYIDDTNRGTLL 
                   
               
               
                   
                   
                 ttcaccgtgcgttctttggccttcaccgctgaagaaaca 
                   
                 WFLIFDATGGAISDI 
                   
               
               
                   
                   
                 ttgtctgaacaaactatcactagagttatgaagtacatc 
                   
                 PMNATAYSHRDKIM 
                   
               
               
                   
                   
                 gacgatacgaacagaggtaccttgttatggtttttaatat 
                   
                 YCQGYGIGLPVLNQ 
                   
               
               
                   
                   
                 tcgacgcaacgggtggtgctataagcgatattcccatg 
                   
                 HTKDFLTGLTDTIQA 
                   
               
               
                   
                   
                 aatgctactgcctactcccacagggacaagatcatgta 
                   
                 SMRQNLTTYPGYVD 
                   
               
               
                   
                   
                 ctgtcaaggctacggtattggtctaccagtcttaaacca 
                   
                 PSLANPQQSYWGPN 
                   
               
               
                   
                   
                 acatactaaagatttccttacgggtcttaccgacactat 
                   
                 LAMLESIKTTYDPN 
                   
               
               
                   
                   
                 ccaggcttctatgagacaaaacttgactacctacccag 
                   
                 DLFHNPQSVRPGNK 
                   
               
               
                   
                   
                 gttatgttgatccttcattggctaatccacaacaatcttat 
                   
                 KASMTQEF 
                   
               
               
                   
                   
                 tggggcccaaaccttgcaatgttggaatcaattaagac 
                   
                   
                   
               
               
                   
                   
                 cacgtatgacccaaacgatttgttccataacccacaat 
                   
                   
                   
               
               
                   
                   
                 ccgttagaccaggtaataaaaaggcttctatgactcaa 
                   
                   
                   
               
               
                   
                   
                 gagttt 
                   
                   
                   
               
               
                   
               
               
                 t807881 
                 Library 
                 atgagtaataacacccttattcaagcttgcctgttagcc 
                 52 
                 MSNNTLIQACLLAA 
                 122 
               
               
                   
                   
                 gctctaggattcaactctactagagtaaaatttcctgaa 
                   
                 LGFNSTRVKFPEAGT 
                   
               
               
                   
                   
                 gcaggtacaatcgacgccgatccatataacttggactt 
                   
                 IDADPYNLDLPIVPA 
                   
               
               
                   
                   
                 gcccatagttccagctgctattactgtcccacgttccac 
                   
                 AITVPRSTAQVANV 
                   
               
               
                   
                   
                 ggctcaggtggccaatgtcgttaagtgtgctgcatcga 
                   
                 VKCAASNGYKVQSR 
                   
               
               
                   
                   
                 acggttacaaggttcaatcaagatctggcggtcacag 
                   
                 SGGHSYGNHGLGGT 
                   
               
               
                   
                   
                 ctacggtaatcatggtttgggcggtaccgatggtgcta 
                   
                 DGAIVVDLKDLKQF 
                   
               
               
                   
                   
                 tcgttgtggatttaaaagacttaaagcaattctctatgga 
                   
                 SMDESTYIASIGSGM 
                   
               
               
                   
                   
                 tgagtccacttacattgcatctatcggttcaggtatgttg 
                   
                 LLDEVTHKLYDNGK 
                   
               
               
                   
                   
                 ttggacgaagtcacccacaagttgtatgataacggtaa 
                   
                 RAMAHGVCPQVGV 
                   
               
               
                   
                   
                 gagagccatggctcatggagtttgtccacaagtcggt 
                   
                 GGHFTIGGLGPTAR 
                   
               
               
                   
                   
                 gtgggtggtcactttacaattggtggtttgggacctact 
                   
                 QWGSSLDHVEEVEV 
                   
               
               
                   
                   
                 gctagacagtggggttcctctcttgaccatgttgaaga 
                   
                 VLANSSIVRASNTQN 
                   
               
               
                   
                   
                 agttgaggtcgttttggccaattctagtatagttagggct 
                   
                 QDVLFAIKGAAASF 
                   
               
               
                   
                   
                 tccaacactcaaaaccaagacgtcctattcgctatcaa 
                   
                 GIVTEFKVRTEPAPD 
                   
               
               
                   
                   
                 aggtgctgctgcttctttcggtattgtgaccgaatttaag 
                   
                 VAVQYTYELILGNIT 
                   
               
               
                   
                   
                 gttagaaccgaaccagccccagatgtcgcagttcaat 
                   
                 ERARIMVDWQDFIS 
                   
               
               
                   
                   
                 acacttatgaattaattttgggtaatatcacagaaagag 
                   
                 DPDLSRKFASIMIVF 
                   
               
               
                   
                   
                 ctaggattatggtcgattggcaggacttcatatccgac 
                   
                 EHGMLLSGDFYGSK 
                   
               
               
                   
                   
                 cccgatctttctcgtaaatttgcatcgattatgatcgtctt 
                   
                 KEFDDLGLADRFPIR 
                   
               
               
                   
                   
                 cgaacacggtatgttgctgtccggcgatttctacggttc 
                   
                 KPGNVAILTDWLGM 
                   
               
               
                   
                   
                 gaagaaggaatttgatgacctgggtttggctgacagat 
                   
                 TGHAVEELALGIVG 
                   
               
               
                   
                   
                 tcccaattagaaagccaggtaacgttgctattttgactg 
                   
                 GIPLHFYAKSMAFTR 
                   
               
               
                   
                   
                 attggttaggaatgacaggtcatgctgttgaggaattg 
                   
                 DSLMSPSTFEKLFDY 
                   
               
               
                   
                   
                 gctttaggtatcgtaggtggcatccctttgcacttttatg 
                   
                 LDNTDKGTLLWLIY 
                   
               
               
                   
                   
                 ccaagagtatggctttcacccgtgacagtttaatgtctc 
                   
                 FDLQSGATSDVPNN 
                   
               
               
                   
                   
                 catctacgttcgaaaaattgtttgattaccttgataacact 
                   
                 STAYAHRDTLYWLQ 
                   
               
               
                   
                   
                 gataagggcaccttgctatggctaatttacttcgatttgc 
                   
                 SYVVNLVGPVSNTT 
                   
               
               
                   
                   
                 aatcaggcgccacttcagacgtaccaaataattctaca 
                   
                 TAFLEGINNLIAQDV 
                   
               
               
                   
                   
                 gcatacgctcacagagacactttgtactggctgcaatc 
                   
                 PSANTRAYPGYVDP 
                   
               
               
                   
                   
                 ctacgtagttaacttggtgggtccagtcagtaacacca 
                   
                 LMPNPQWRYWGSN 
                   
               
               
                   
                   
                 ccacagcttttcttgaaggtataaataacttgatcgccc 
                   
                 LPKLEKIKAAIDPND 
                   
               
               
                   
                   
                 aagacgtaccttccgccaacactagagcttatccaggt 
                   
                 VFHNPQSVKPRKQA 
                   
               
               
                   
                   
                 tacgttgatccattaatgcccaacccacaatggcgttat 
                   
                 P 
                   
               
               
                   
                   
                 tggggttcgaatttaccaaagttggaaaagataaaagc 
                   
                   
                   
               
               
                   
                   
                 agctattgaccctaatgacgtcttccataacccacaatc 
                   
                   
                   
               
               
                   
                   
                 agtcaagcctagaaaacaagcaccc 
                   
                   
                   
               
               
                   
               
               
                 t807883 
                 Library 
                 atgggacagagccaagcattgtcagcctttaaaaagg 
                 53 
                 MGQSQALSAFKKDL 
                 123 
               
               
                   
                   
                 acctagctgctgctcttaataacgatgatgaattgttcg 
                   
                 AAALNNDDELFALP 
                   
               
               
                   
                   
                 ctctgccagacgagcctttctacattaaggaccacgtc 
                   
                 DEPFYIKDHVKRYN 
                   
               
               
                   
                   
                 aagcgttataacttagatatcgaaaccacacccttagc 
                   
                 LDIETTPLAVTYPKT 
                   
               
               
                   
                   
                 cgtaacgtacccaaaaactaccgcacaagtttcttcca 
                   
                 TAQVSSIVKLAKDN 
                   
               
               
                   
                   
                 tagtgaagttggctaaggataacaatttgaaagttcaa 
                   
                 NLKVQAKCGGHSY 
                   
               
               
                   
                   
                 gctaagtgcggtggtcattcttatgccaatttttgtgatc 
                   
                 ANFCDPNGGIIVDLK 
                   
               
               
                   
                   
                 caaacggtggcatcattgtcgaccttaaacacttccaa 
                   
                 HFQKFEIDENTWRC 
                   
               
               
                   
                   
                 aagtttgaaattgacgaaaacacttggagatgtagggt 
                   
                 RVGGGTLLGDLTKR 
                   
               
               
                   
                   
                 cggtggtggtactttgctaggtgatttgactaagagaat 
                   
                 MFEPHKRAMAHGT 
                   
               
               
                   
                   
                 gttcgaaccacataagagagctatggctcacggtacc 
                   
                 CPTVGIGGHATIGGL 
                   
               
               
                   
                   
                 tgtccaacagttggtatcggaggtcatgcaaccatcgg 
                   
                 GPSSRLWGAALDHV 
                   
               
               
                   
                   
                 tggcttgggtcctagttcgagattatggggtgctgcctt 
                   
                 EEVEMVVANGDVIR 
                   
               
               
                   
                   
                 agaccacgtagaggaagtcgaaatggttgttgctaac 
                   
                 ANENENSDIFWAVK 
                   
               
               
                   
                   
                 ggtgacgttattagagccaacgaaaacgaaaattccg 
                   
                 GAGASFGVITEFVVR 
                   
               
               
                   
                   
                 atatattctgggctgtcaaaggcgctggtgcctcttttg 
                   
                 TEPAPGRLVQYSYSF 
                   
               
               
                   
                   
                 gtgttattactgaatttgttgtaagaactgagccagctcc 
                   
                 TTGSWKDMAKTFK 
                   
               
               
                   
                   
                 aggtagattggttcaatactcttattccttcacaaccggt 
                   
                 AWQTYVSQPDLTRS 
                   
               
               
                   
                   
                 tcatggaaggacatggccaagactttcaaggcttggc 
                   
                 FASTATITELGLTISV 
                   
               
               
                   
                   
                 aaacctacgtcagccaacctgatttgacgcgtagtttc 
                   
                 TYFGTDEEFDKINFA 
                   
               
               
                   
                   
                 gcaagtactgctacgatcaccgaactgggtttgactatt 
                   
                 KNFPGNQTPKTIVFD 
                   
               
               
                   
                   
                 tctgtcacatacttcggcactgacgaagaatttgataaa 
                   
                 DYLGAVGHWAEDV 
                   
               
               
                   
                   
                 ataaatttcgctaaaaatttcccaggtaaccagacccc 
                   
                 ALEIISPLPAHSYTKT 
                   
               
               
                   
                   
                 aaagaccatcgtttttgatgattacttgggtgctgtggg 
                   
                 LTFNHCNQIPDSVID 
                   
               
               
                   
                   
                 acattgggccgaagatgttgctttagaaattatctctcct 
                   
                 RMFKYFEEVSKGTL 
                   
               
               
                   
                   
                 ttgcccgcccactcctatacaaagactttgacttttaacc 
                   
                 VWFAIFDLAGGRVN 
                   
               
               
                   
                   
                 actgcaaccaaattccagactctgtgattgatagaatgt 
                   
                 DIPQDATAYAHRDA 
                   
               
               
                   
                   
                 tcaaatacttcgaggaagtttcgaagggtacgttagttt 
                   
                 LFYLQSYAVNPFGP 
                   
               
               
                   
                   
                 ggtttgccatcttcgatttggctggtggtagagtcaatg 
                   
                 VSNKSKQFLQGLNK 
                   
               
               
                   
                   
                 acatcccacaagacgctaccgcatatgctcatagaga 
                   
                 VIRDGMAEAGENTD 
                   
               
               
                   
                   
                 tgctttgttctacttacaatcctacgctgtgaacccatttg 
                   
                 LGAYAGYVDLELGA 
                   
               
               
                   
                   
                 gtcccgtttctaataaaagtaagcaatttctgcaaggcc 
                   
                 GAQKAYWRTNLPR 
                   
               
               
                   
                   
                 ttaacaaggtcatccgtgatggaatggctgaagctggt 
                   
                 LESIKLKWDPEDVF 
                   
               
               
                   
                   
                 gaaaatacagacttgggtgcatatgccggctacgttga 
                   
                 HNPQSVRPGGNDVI 
                   
               
               
                   
                   
                 tctggaattaggtgctggtgctcagaaggcctactgga 
                   
                 STPKVVYKKAGFLA 
                   
               
               
                   
                   
                 gaactaacttgccacgtttggagtctattaagctaaagt 
                   
                 RLKGCFR 
                   
               
               
                   
                   
                 gggacccagaggatgtattccacaatcctcaatccgtc 
                   
                   
                   
               
               
                   
                   
                 agaccaggtggtaacgacgttatttctaccccaaaggt 
                   
                   
                   
               
               
                   
                   
                 agtctacaaaaaggctggtttcctagctaggttaaaag 
                   
                   
                   
               
               
                   
                   
                 gttgtttcaga 
                   
                   
                   
               
               
                   
               
               
                 t807917 
                 Library 
                 atgggtaatacaaccagcattgctggaagggattgcct 
                 54 
                 MGNTTSIAGRDCLV 
                 124 
               
               
                   
                   
                 agtctctgcattgggcggtaacgccgacttagttgcttt 
                   
                 SALGGNADLVAFQN 
                   
               
               
                   
                   
                 tcaaaaccagttgctttaccaaactactgctgtgcacga 
                   
                 QLLYQTTAVHEYNL 
                   
               
               
                   
                   
                 gtataatctgaacatacccgttacgcctgccgctatcac 
                   
                 NIPVTPAAITYPETA 
                   
               
               
                   
                   
                 ctacccagaaactgctgaacaaattgctgctgtcgtta 
                   
                 EQIAAVVKCASEYD 
                   
               
               
                   
                   
                 aatgtgcctccgaatacgattataaggtacaagccaga 
                   
                 YKVQARSGGHSFGN 
                   
               
               
                   
                   
                 tcaggtggtcattctttcggtaattacggtttgggtggaa 
                   
                 YGLGGTDGAVVVD 
                   
               
               
                   
                   
                 ccgacggtgctgttgtcgttgatatgaagcacttcaac 
                   
                 MKHFNQFSMDDQT 
                   
               
               
                   
                   
                 caatttagtatggacgatcaaacttatgaagctgttttag 
                   
                 YEAVLGPGTTLGDV 
                   
               
               
                   
                   
                 gtccaggtactaccttgggcgacgtcgatacagaattg 
                   
                 DTELYNNGKRAMA 
                   
               
               
                   
                   
                 tacaacaacggtaagcgtgctatggcacatggtatctg 
                   
                 HGICPTISTGGHFTM 
                   
               
               
                   
                   
                 tccaacgatttcaaccggtggtcacttcactatgggtg 
                   
                 GGLGPTARQWGLAL 
                   
               
               
                   
                   
                 gcttgggtccaaccgccagacaatggggtttagctctt 
                   
                 DHVEEVEVILANSSI 
                   
               
               
                   
                   
                 gaccatgtcgaagaagtcgaggttatccttgctaattct 
                   
                 VRASNTQNQEVFFA 
                   
               
               
                   
                   
                 tccatcgtaagagcctcgaacacccagaatcaagaag 
                   
                 VKGAAASFGIVTEF 
                   
               
               
                   
                   
                 ttttctttgcagttaaaggagctgccgctagtttcggtatt 
                   
                 KVRTQPAPGLAVQY 
                   
               
               
                   
                   
                 gtcacagagtttaaggtcagaactcaaccagcacctg 
                   
                 SYTFNLGSSAKKAQ 
                   
               
               
                   
                   
                 gtttggctgttcagtattcttacaccttcaacttgggttctt 
                   
                 FVKDWQSFISAKNL 
                   
               
               
                   
                   
                 ccgctaagaaagctcaattcgttaaggattggcaaag 
                   
                 TRQFYTNMVIFDGDI 
                   
               
               
                   
                   
                 ctttatatccgctaaaaatctaactagacaattttacacta 
                   
                 ILEGLFFGSKEQYEA 
                   
               
               
                   
                   
                 acatggtaatcttcgacggtgatattattttggaaggctt 
                   
                 LGLEERFVPKNPGNI 
                   
               
               
                   
                   
                 attcttcggctctaaggaacaatacgaagcactgggttt 
                   
                 LVLTDWLGMVGHA 
                   
               
               
                   
                   
                 ggaagaacgttttgttccaaagaatccaggtaacatctt 
                   
                 LEDTILRLVGNTPTW 
                   
               
               
                   
                   
                 ggttctaacagactggttgggtatggtgggtcacgcct 
                   
                 FYAKSLGFTPDTLIP 
                   
               
               
                   
                   
                 tggaagacactatattgagacttgtcggtaacactccta 
                   
                 SAGIDEFFEYIENNK 
                   
               
               
                   
                   
                 cctggttttacgcaaagagcttgggtttcactccagata 
                   
                 AGTSTWFVTLSLEG 
                   
               
               
                   
                   
                 cgttaattccttctgctggtattgatgaatttttcgaatata 
                   
                 GAINDVPADATAYG 
                   
               
               
                   
                   
                 tcgaaaacaacaaggctggcacatccacctggtttgtc 
                   
                 HRDVLFWVQIFMVS 
                   
               
               
                   
                   
                 accttatctttagaaggtggtgccattaatgacgtacca 
                   
                 PTGPVSSTTYDFADG 
                   
               
               
                   
                   
                 gctgatgctacggcatacggtcacagagatgtgttgtt 
                   
                 LYNVLTKAVPESEG 
                   
               
               
                   
                   
                 ctgggttcagattttcatggtcagtccaactggaccagt 
                   
                 HAYLGCPDPKMAN 
                   
               
               
                   
                   
                 ttcgtctaccacttatgacttcgctgatggtctgtacaac 
                   
                 AQQKYWRQNLPRL 
                   
               
               
                   
                   
                 gtcttgaccaaagctgtgccagagagtgagggtcatg 
                   
                 EELKAILDPKDTFHN 
                   
               
               
                   
                   
                 cttacttgggttgtcccgatccaaaaatggccaatgctc 
                   
                 PQGILPA 
                   
               
               
                   
                   
                 aacaaaagtattggagacaaaaccttcctagactgga 
                   
                   
                   
               
               
                   
                   
                 agaattgaaggctatcttagatccaaaggacacttttca 
                   
                   
                   
               
               
                   
                   
                 taacccacaaggaattttacccgcc 
                   
                   
                   
               
               
                   
               
               
                 t807918 
                 Library 
                 atgggtaataccacatccatcgccgctggacgtgattg 
                 55 
                 MGNTTSIAAGRDCL 
                 125 
               
               
                   
                   
                 cctattgtcggctgttggcggtaaccacgcacatgtcg 
                   
                 LSAVGGNHAHVAFQ 
                   
               
               
                   
                   
                 ccttccaggaccaattattgtatcaagctactgctgtgg 
                   
                 DQLLYQATAVEPYN 
                   
               
               
                   
                   
                 agccatacaaccttaacatacctgttactccagctgctg 
                   
                 LNIPVTPAAVTYPQS 
                   
               
               
                   
                   
                 tcacgtacccccaaagcgcagacgaaattgccgctgt 
                   
                 ADEIAAVVKCAAEY 
                   
               
               
                   
                   
                 agttaagtgtgctgctgaatacggttataaagtccaag 
                   
                 GYKVQARSGGHSFG 
                   
               
               
                   
                   
                 caagatcaggtggtcactcttttggcaattacggtctgg 
                   
                 NYGLGGEDGAIVVE 
                   
               
               
                   
                   
                 gtggtgaagatggtgccattgttgttgaaatgaagcatt 
                   
                 MKHFNQFSMDESTY 
                   
               
               
                   
                   
                 tcaaccaattttctatggacgaaagtacctatactgcta 
                   
                 TATIGPGITLGDLDT 
                   
               
               
                   
                   
                 ccatcggcccaggtattactcttggtgatttggatacag 
                   
                 GLYNAGHRAMAHG 
                   
               
               
                   
                   
                 gtttgtacaacgccggtcacagggcaatggctcatgg 
                   
                 ICPTIRTGGHLTMGG 
                   
               
               
                   
                   
                 tatctgtccaactattagaaccggaggtcacttgactat 
                   
                 LGPTARQWGLALDH 
                   
               
               
                   
                   
                 gggtggtttaggtccaacagctagacagtggggatta 
                   
                 VEEVEVVLANSSIVR 
                   
               
               
                   
                   
                 gctttggaccatgttgaagaggtcgaagtggttttggc 
                   
                 ASDTQNQDIFFAVK 
                   
               
               
                   
                   
                 aaattcctctattgtcagagctagcgacacccaaaatc 
                   
                 GAAASFGIVTEFKVR 
                   
               
               
                   
                   
                 aagatatattcttcgctgttaagggtgccgctgcctcttt 
                   
                 TEEAPGLAVQYSFTF 
                   
               
               
                   
                   
                 tggtatcgtaactgaatttaaagtcagaaccgaagaag 
                   
                 NLGTAAEKAKLVKD 
                   
               
               
                   
                   
                 ctcctggattagctgtccaatactccttcactttcaacttg 
                   
                 WQAFIAQEDLTWKF 
                   
               
               
                   
                   
                 ggtaccgccgccgaaaaggctaaacttgttaaggact 
                   
                 YSNMNIFDGQIILEGI 
                   
               
               
                   
                   
                 ggcaagctttcattgctcaagaggatttgacctggaag 
                   
                 YFGSKEEYDALGLE 
                   
               
               
                   
                   
                 ttttactccaacatgaacatcttcgatggtcaaataatctt 
                   
                 ERFPTSEPGTVLVLT 
                   
               
               
                   
                   
                 agaaggtatttactttggttctaaggaagaatatgatgc 
                   
                 DWLGMVGHGLEDV 
                   
               
               
                   
                   
                 attgggtttagaagagagattcccaacctctgaacctg 
                   
                 ILRLVGNTPTWFYA 
                   
               
               
                   
                   
                 gtactgttctggtgttgacagactggttgggtatggttg 
                   
                 KSLGFAPRALIPDSAI 
                   
               
               
                   
                   
                 gacacggcctagaggatgtcattttgaggttagtgggt 
                   
                 DDFFSYIHENNPGTV 
                   
               
               
                   
                   
                 aatactccaacttggttttatgccaaatcactaggtttcg 
                   
                 SWFVTLSLEGGAIN 
                   
               
               
                   
                   
                 ccccacgtgccttgatcccagacagtgctattgatgatt 
                   
                 KVPEDATAYGHRDV 
                   
               
               
                   
                   
                 tcttttcttatatacacgaaaacaacccaggtactgtttct 
                   
                 LFWVQIFMINPLGPV 
                   
               
               
                   
                   
                 tggttcgtaacgcttagcttggaaggtggcgctatcaa 
                   
                 SQTTYGFADGLYDV 
                   
               
               
                   
                   
                 caaggttcccgaagacgctaccgcttacggtcacaga 
                   
                 LAKAVPESAGHAYL 
                   
               
               
                   
                   
                 gatgtgttgttttgggtacaaattttcatgattaatccttta 
                   
                 GCPDPRMPNAQQAY 
                   
               
               
                   
                   
                 ggtccagtttcgcagactacctacggtttcgcagacgg 
                   
                 WRSNLPRLEELKGE 
                   
               
               
                   
                   
                 attgtacgacgtcctagctaaggctgtcccagaatcag 
                   
                 LDPKDIFHNPQGVM 
                   
               
               
                   
                   
                 ctggtcatgcatacctgggttgtcccgacccacgtatg 
                   
                 VVS 
                   
               
               
                   
                   
                 ccaaacgcccaacaagcttattggagatccaacttgc 
                   
                   
                   
               
               
                   
                   
                 caagattggaagaattaaaaggtgaattggatccaaa 
                   
                   
                   
               
               
                   
                   
                 ggatatctttcataatccacagggtgttatggttgtttct 
                   
                   
                   
               
               
                   
               
               
                 t807926 
                 Library 
                 atgggaaataccactagcattgcaggtcgtgactgcct 
                 56 
                 MGNTTSIAGRDCLIS 
                 126 
               
               
                   
                   
                 aatatctgccttaggtggtaactcagctcttgctgctttc 
                   
                 ALGGNSALAAFPNQ 
                   
               
               
                   
                   
                 cctaaccaactgttgtggacggccgatgtacacgaat 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                   
                 ataatttaaacttgccagttacaccagctgctatcactta 
                   
                 PVTPAAITYPETAEQ 
                   
               
               
                   
                   
                 ccccgagactgccgaacagattgcaggcatcgtcaa 
                   
                 IAGIVKCASDYDYK 
                   
               
               
                   
                   
                 gtgtgcttccgactacgattacaaagtgcaagctaggt 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 ctggtggtcatagttttggtaattatggtttgggcggaa 
                   
                 LGGTDGAVVVDMK 
                   
               
               
                   
                   
                 ccgacggtgccgtcgttgttgatatgaagcacttcaac 
                   
                 HFNQFSMDDQTYEA 
                   
               
               
                   
                   
                 caattttcaatggacgatcaaacctacgaagctgttatt 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 ggtccaggtacaactttgaacgatgttgatatagaatta 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 tacaataacggtaagagagccatggctcatggcgtct 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 gtcctactatcaaaaccggaggtcacttcactattggtg 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 gtttgggtccaaccgctagacaatggggtcttgctttg 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 gaccacgtagaagaggtcgaagtcgttttggctaactc 
                   
                 NTQNQDVLFAVKG 
                   
               
               
                   
                   
                 ttccatcgttagagcaagtaatacccaaaaccaagatg 
                   
                 AAADFGIVTEFKVR 
                   
               
               
                   
                   
                 tcttgttcgccgttaagggtgctgccgctgactttggaa 
                   
                 TEPAPGLAVQYSYT 
                   
               
               
                   
                   
                 ttgtaaccgaatttaaggttagaactgaaccagctcca 
                   
                 FNLGSTAEKAQFVK 
                   
               
               
                   
                   
                 ggtttggccgttcagtattcgtatacgttcaacctaggtt 
                   
                 DWQSFISAKNLTRQ 
                   
               
               
                   
                   
                 ctactgctgaaaaagctcaattcgtgaaggactggca 
                   
                 FYNNMVIFDGDIILE 
                   
               
               
                   
                   
                 atctttcatttccgctaaaaatttaaccagacaattttaca 
                   
                 GLFFGSKEQYDALG 
                   
               
               
                   
                   
                 acaatatggtcatcttcgatggtgatatcattctggagg 
                   
                 LEDHFAPKNPGNILV 
                   
               
               
                   
                   
                 gtttgttctttggtagcaaggaacaatacgatgccctag 
                   
                 LTDWLGMVGHALE 
                   
               
               
                   
                   
                 gtttggaagaccatttcgcacccaagaacccaggtaa 
                   
                 DTILKLVGNTPTWF 
                   
               
               
                   
                   
                 catcctggttttaaccgactggcttggcatggtcggcc 
                   
                 YAKSLGFRQDTLIPS 
                   
               
               
                   
                   
                 acgctttggaagatacaatacttaagttggtcggtaata 
                   
                 AGIDEFFEYIDNHTA 
                   
               
               
                   
                   
                 ctccaacttggttttatgccaagtctttgggtttcagaca 
                   
                 GTPAWFVTLSLEGG 
                   
               
               
                   
                   
                 agatactttgattccttccgctggtattgatgaatttttcg 
                   
                 AINDVAEDATAYAH 
                   
               
               
                   
                   
                 aatacatagacaaccacacggctggtactccagcttg 
                   
                 RDVLFWVQLFMVN 
                   
               
               
                   
                   
                 gttcgttacattatcattggagggtggtgccatcaatga 
                   
                 PLGPISETTYEFTDG 
                   
               
               
                   
                   
                 cgtggccgaagatgctactgcatacgcccatcgtgat 
                   
                 LYDVLARAVPESVG 
                   
               
               
                   
                   
                 gttttattctgggttcagttgtttatggtcaacccacttgg 
                   
                 HAYLGCPDPRMENA 
                   
               
               
                   
                   
                 tccaatctctgaaacaacctacgaatttacggatggttt 
                   
                 PQKYWRTNLPRLQE 
                   
               
               
                   
                   
                 gtatgacgttctagctagagctgttcctgagtctgttggt 
                   
                 LKEELDPKNTFHHP 
                   
               
               
                   
                   
                 catgcctacttgggatgtccagatccacgtatggaaaa 
                   
                 QGVIPA 
                   
               
               
                   
                   
                 cgcacctcagaagtactggagaactaatttacctagat 
                   
                   
                   
               
               
                   
                   
                 tgcaagaactgaaggaagaattggacccaaaaaata 
                   
                   
                   
               
               
                   
                   
                 cattccaccatccacaaggtgttattccagct 
                   
                   
                   
               
               
                   
               
               
                 t807928 
                 Library 
                 atgggtaataccacatctattgccggcagagactgcct 
                 57 
                 MGNTTSIAGRDCLIS 
                 127 
               
               
                   
                   
                 aatcagcgctttaggtggagattccgcactggctgtctt 
                   
                 ALGGDSALAVFPNQ 
                   
               
               
                   
                   
                 cccaaaccagcttttgtggactgctgatgtgcacgaat 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                   
                 acaacttaaatcttcctgtaactccagccgctataacct 
                   
                 PVTPAAITYPETAEQ 
                   
               
               
                   
                   
                 atcccgagacagctgaacaaattgccggtatcgttaaa 
                   
                 IAGIVKCASDYDYK 
                   
               
               
                   
                   
                 tgtgcttcagactacgattataaggttcaagcacgtagt 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 ggtggtcattcctttggcaactacggtttgggtggtact 
                   
                 LGGTDGAVVVDMK 
                   
               
               
                   
                   
                 gacggtgctgttgtcgtcgacatgaagcacttcaatca 
                   
                 HFNQFSMDDQTYEA 
                   
               
               
                   
                   
                 attttctatggatgatcaaacctacgaagcagttattggt 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 ccaggtactaccttgaacgacgttgacatcgaattgta 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 caacaatggaaagagagctatggctcatggtgtatgtc 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 caaccataaaaactggtggtcatttcacgattggtggtt 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 tgggtcctacggccagacaatggggcttggctttagat 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 cacgttgaagaagttgaggtcgtcttggccaactcttc 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 gatcgtcagggcttctaatactcaaaaccaagatgtctt 
                   
                 AADFGIVTEFKVRTE 
                   
               
               
                   
                   
                 tttcgctgttaagggcgccgcagctgacttcggtattgt 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 gactgaatttaaggttagaacagaaccagctccagga 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 ttggccgtgcagtatagctatactttcaaccttggtagta 
                   
                 QSFISAKNLTRQFYN 
                   
               
               
                   
                   
                 ccgctgaaaaagctcaattcgttaaggattggcaaag 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 ctttatctccgccaagaacttgacgagacaattctacaa 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 taatatggtcattttcgacggtgatattatcttagagggtt 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 tgttctttggttcgaaggaacaatacgacgctttgggttt 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 ggaagaccactttgcaccaaaaaacccaggtaacatt 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 ctagttctaaccgattggttaggtatggtaggacacgct 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 ttagaagatactatcttgaagctagttggtaataccccc 
                   
                 EFFEYIANHTTGTPV 
                   
               
               
                   
                   
                 acttggttctatgcaaagtctttgggttttagacaggaca 
                   
                 WLVTLSLEGGAIND 
                   
               
               
                   
                   
                 cactgatcccttctgctggaattgatgaatttttcgaata 
                   
                 VAEDATAYAHRDV 
                   
               
               
                   
                   
                 cattgctaaccacaccaccggtactcctgtttggctggt 
                   
                 LFWVQLFMVNPLGP 
                   
               
               
                   
                   
                 tactttgtcattagaaggtggtgccattaatgatgtagct 
                   
                 ISETTYEFTDGLYDV 
                   
               
               
                   
                   
                 gaggatgcaacagcttacgctcatagagatgtcctattt 
                   
                 LARAVPESVGHAYL 
                   
               
               
                   
                   
                 tgggttcaattgttcatggttaacccattgggtcctatttc 
                   
                 GCPDPRMEDAPQKY 
                   
               
               
                   
                   
                 tgaaacaacttatgaatttacagacggattgtacgacgt 
                   
                 WRTNLPRLQELKEE 
                   
               
               
                   
                   
                 cttggcccgtgctgtcccagagtccgtcggtcatgcct 
                   
                 LDPKNTFHHPQGVIP 
                   
               
               
                   
                   
                 acttaggctgtccagacccaagaatggaagatgctcc 
                   
                 A 
                   
               
               
                   
                   
                 acaaaagtactggcgtaccaacttgccaagattgcaa 
                   
                   
                   
               
               
                   
                   
                 gaattgaaggaagaattagacccaaaaaacacgttcc 
                   
                   
                   
               
               
                   
                   
                 accatccacaaggtgttatacccgcc 
                   
                   
                   
               
               
                   
               
               
                 t807929 
                 Library 
                 atgggtaataaagcaagtaccacaacgataatcacca 
                 58 
                 MGNKASTTTIITTAV 
                 128 
               
               
                   
                   
                 ctgctgtacacaagtgccttctgtcggccgtgaacggc 
                   
                 HKCLLSAVNGNSAQ 
                   
               
               
                   
                   
                 aactcagctcaggtttccgtccaaaacgacttattgtac 
                   
                 VSVQNDLLYGVTAV 
                   
               
               
                   
                   
                 ggtgttaccgctgttcatgaatataatttgaactttccaat 
                   
                 HEYNLNFPMTPAAV 
                   
               
               
                   
                   
                 gactcccgctgccgtcactttccctgagacttccgaac 
                   
                 TFPETSEQVAALVK 
                   
               
               
                   
                   
                 aagttgctgcattggtcaagtgtgctgccgaatacaag 
                   
                 CAAEYKYKVQARS 
                   
               
               
                   
                   
                 tataaagtgcaagctaggagcggaggtcactctttcg 
                   
                 GGHSFGNHGLGGAD 
                   
               
               
                   
                   
                 gtaaccatggtctaggtggtgctgatggagctattgttg 
                   
                 GAIVVDMKHFQQFS 
                   
               
               
                   
                   
                 tcgatatgaagcactttcaacaattctctatggacaatg 
                   
                 MDNETHVATIGPGL 
                   
               
               
                   
                   
                 aaacccacgttgccacaattggcccaggtttgagtcta 
                   
                 SLGDIDTLLYNAGG 
                   
               
               
                   
                   
                 ggtgacatcgatacacttttgtacaacgctggtggtag 
                   
                 RAMSHGICPEIRAGG 
                   
               
               
                   
                   
                 agccatgagccatggtatttgtccagaaatacgtgccg 
                   
                 HLTIGGLGLTSRQW 
                   
               
               
                   
                   
                 gaggtcacttaactatcggtggtttgggtttgacttctcg 
                   
                 GMSLDHIEEVEVVL 
                   
               
               
                   
                   
                 tcaatggggtatgtctttagaccatatcgaagaagtcg 
                   
                 PNSSIVRASETENAD 
                   
               
               
                   
                   
                 aggtagttttgccaaattcctcgatcgttagagcttctga 
                   
                 LLFAVKGAAASFGV 
                   
               
               
                   
                   
                 aaccgaaaatgctgatctattattcgctgttaagggcgc 
                   
                 VTEFKVRTQLAPKE 
                   
               
               
                   
                   
                 agctgcatcttttggtgttgtcactgaatttaaggtaaga 
                   
                 AIQYSYSFKLGSAAQ 
                   
               
               
                   
                   
                 acgcaacttgcacctaaagaagctattcagtactcata 
                   
                 RARLFADWQDLALR 
                   
               
               
                   
                   
                 cagtttcaaattgggttccgctgcccaaagagctagatt 
                   
                 RDLSRKFTSDFICLQ 
                   
               
               
                   
                   
                 gttcgctgattggcaagacttggcattaaggagagattt 
                   
                 DSVIVKGVFFGSKKE 
                   
               
               
                   
                   
                 gtctcgtaagttcacatccgatttcatttgtttgcaagact 
                   
                 YNALRIEHHLPGSDS 
                   
               
               
                   
                   
                 ctgtcattgtgaagggtgtgtttttcggttccaaaaagg 
                   
                 SKVLVLDDWLGIVT 
                   
               
               
                   
                   
                 aatataacgccctaagaattgaacatcacttaccaggc 
                   
                 HVVDDLAVRLGGS 
                   
               
               
                   
                   
                 tctgacagttctaaggttttggtcttagatgactggttgg 
                   
                 MSTYFYAKSLGFTR 
                   
               
               
                   
                   
                 gtattgttacccacgttgtcgatgatctggctgttagatt 
                   
                 DTLMPPSTITSLFTY 
                   
               
               
                   
                   
                 aggtggttccatgtcaacttacttttatgccaagtcactt 
                   
                 LDKAKKGTITWFVT 
                   
               
               
                   
                   
                 ggttttaccagagatactttgatgccaccatcgacgatc 
                   
                 FSLVGGAINDYPKN 
                   
               
               
                   
                   
                 acctctttattcacttacttggacaaagctaagaaaggc 
                   
                 ATAYPHRDVIYWM 
                   
               
               
                   
                   
                 acaataacttggttcgtcaccttcagcttggtcggtggt 
                   
                 QSFAINALGPVLNST 
                   
               
               
                   
                   
                 gctatcaatgattaccctaagaacgccacggcttatcc 
                   
                 YDFLDGINELVARD 
                   
               
               
                   
                   
                 acacagagatgttatctactggatgcaatcttttgctatt 
                   
                 LPGCAGHAYLGCPD 
                   
               
               
                   
                   
                 aacgctctgggtcctgttttgaactccacttacgacttct 
                   
                 PRMEGAERAYWGS 
                   
               
               
                   
                   
                 tggacggcatcaatgagctagtcgcacgtgatttacca 
                   
                 NLGRLEDMKGVFDP 
                   
               
               
                   
                   
                 ggttgtgccggacacgcttatttaggttgcccagatcc 
                   
                 VDVFWNPQGVGVP 
                   
               
               
                   
                   
                 cagaatggagggtgctgaaagagcctattggggttca 
                   
                 VA 
                   
               
               
                   
                   
                 aacttaggtagacttgaagacatgaaaggtgtctttga 
                   
                   
                   
               
               
                   
                   
                 cccagttgacgttttctggaatccacaaggcgtcggtg 
                   
                   
                   
               
               
                   
                   
                 tccctgttgct 
                   
                   
                   
               
               
                   
               
               
                 t807930 
                 Library 
                 atgggtaacaccacttccatagcaggccgtgattgcct 
                 59 
                 MGNTTSIAGRDCLIS 
                 129 
               
               
                   
                   
                 aattagcgctcttggtggtaatagtgctctggccgtgtt 
                   
                 ALGGNSALAVFPNQ 
                   
               
               
                   
                   
                 ccccaaccagttattgtggacagctgacgtccatgaat 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                   
                 acaatttgaacttacctgttactccagcagctatcacgt 
                   
                 PVTPAAITYPETAEQ 
                   
               
               
                   
                   
                 atccagagactgctgaacaaatcgctggaattgttaaa 
                   
                 IAGIVKCASDYDYK 
                   
               
               
                   
                   
                 tgtgcctctgattacgactataaggttcaagctaggtct 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 ggtggtcactcctttggtaactacggtttgggcggtac 
                   
                 LGGTDGAVVVDMK 
                   
               
               
                   
                   
                 cgacggtgccgtcgtagtcgatatgaagcacttcactc 
                   
                 HFTQFSMDDQTYEA 
                   
               
               
                   
                   
                 aattttctatggatgaccaaacctacgaagcagttatag 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 gtccaggaacaactttgaatgacgttgatattgaattgt 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 ataacaacggtaaaagagctatggctcatggtgtttgt 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 ccaaccatcaagacaggtggtcacttcactattggtgg 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 tttaggtccaaccgccagacaatggggattggctttag 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 accacgtcgaggaagttgaagtcgttttggctaactca 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 tcgatcgtcagagccagcaatacccaaaatcaggatg 
                   
                 AADFGIVTEFKVRTE 
                   
               
               
                   
                   
                 tctttttcgctgtaaagggtgcagctgccgacttcggca 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 tcgttactgaatttaaagttagaaccgaacctgctccag 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 gtttggccgtgcaatactcgtatacattcaacctaggtt 
                   
                 QSFISAKNLTRQFYN 
                   
               
               
                   
                   
                 ccacggctgagaaggctcaattcgtcaaggattggca 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 atcttttattagtgcaaagaacttgactagacaattctac 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 aacaacatggttattttcgacggtgatattatcttggaag 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 gtttgttctttggctcaaaagaacagtacgatgctcttgg 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 tttggaagatcatttcgctccaaagaatccaggcaaca 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 tcttagttttgactgactggctgggtatggtgggtcacg 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 ctctggaagatacgattttgaagcttgtcggtaataccc 
                   
                 EFFEYIDNHTAGTPA 
                   
               
               
                   
                   
                 ccacctggttctatgctaagtctctaggttttagacaag 
                   
                 WFVTLSLEGGAIND 
                   
               
               
                   
                   
                 ataccctgattcctagtgctggcatcgatgagttctttga 
                   
                 VAEDATAYAHRDV 
                   
               
               
                   
                   
                 atacatcgacaatcacactgccggaactccagcttggt 
                   
                 LFWVQLFMVNPLGP 
                   
               
               
                   
                   
                 tcgtaactttatccttggaaggaggtgccataaatgatg 
                   
                 ISETTYEFTDGLYDV 
                   
               
               
                   
                   
                 ttgccgaagacgctactgcctatgctcatagagatgttt 
                   
                 LARAVPESVGHAYL 
                   
               
               
                   
                   
                 tattttgggttcaattgtttatggtcaaccctttgggtcca 
                   
                 GCPDPRMENAPQKY 
                   
               
               
                   
                   
                 atatctgaaactacatacgaatttactgatggtttatacg 
                   
                 WRTNLPRLQELKEE 
                   
               
               
                   
                   
                 acgtattggccagagcagtaccagaatccgttggtcat 
                   
                 LDPKNTFHHPQGVIP 
                   
               
               
                   
                   
                 gcttaccttggttgtccagacccacgtatggaaaatgc 
                   
                 A 
                   
               
               
                   
                   
                 acctcaaaagtactggaggactaacttgcccagacttc 
                   
                   
                   
               
               
                   
                   
                 aggaattgaaagaagagctagacccaaagaacacct 
                   
                   
                   
               
               
                   
                   
                 tccaccatccacaaggtgtcattccagct 
                   
                   
                   
               
               
                   
               
               
                 t807933 
                 Library 
                 atgggcaacaccacatctatcgctggtagggactgctt 
                 60 
                 MGNTTSIAGRDCLV 
                 130 
               
               
                   
                   
                 ggtatcagccctgggtggtaatgctggtcttgttgcattt 
                   
                 SALGGNAGLVAFQN 
                   
               
               
                   
                   
                 caaaaccagcctttgtatcaaactactgctgtgcacga 
                   
                 QPLYQTTAVHEYNL 
                   
               
               
                   
                   
                 atacaatttaaacataccagttaccccagccgctattac 
                   
                 NIPVTPAAITYPETA 
                   
               
               
                   
                   
                 gtacccagagactgctgaacaaattgccgctgtcgtc 
                   
                 EQIAAVVKCASQYD 
                   
               
               
                   
                   
                 aagtgtgcatcccaatacgattataaagtccaagctag 
                   
                 YKVQARSGGHSFGN 
                   
               
               
                   
                   
                 aagtggaggtcatagcttcggtaattacggtctaggcg 
                   
                 YGLGGTDGAVVVD 
                   
               
               
                   
                   
                 gtacagatggtgctgttgttgttgacatgaagtacttca 
                   
                 MKYFNQFSMDDQT 
                   
               
               
                   
                   
                 accaattttctatggacgatcagacctacgaagctgtc 
                   
                 YEAVIGPGTTLGDV 
                   
               
               
                   
                   
                 atcggtcctggtacaaccttgggagatgtcgacgtcg 
                   
                 DVELYNNGKRAMA 
                   
               
               
                   
                   
                 aattatataacaacggtaagcgtgccatggctcacggt 
                   
                 HGVCPTISTGGHFT 
                   
               
               
                   
                   
                 gtttgtccaactatttcgactggaggtcatttcactatgg 
                   
                 MGGLGPTARQWGL 
                   
               
               
                   
                   
                 gtggtttgggtcccaccgccagacaatggggcttagc 
                   
                 ALDHVEEVEVVLAN 
                   
               
               
                   
                   
                 cttggaccacgttgaagaagtagaagtagttttagcaa 
                   
                 SSIVRASNTQNQEVF 
                   
               
               
                   
                   
                 actcctctatcgtgagagctagcaatacgcaaaatcaa 
                   
                 FAVKGAAASFGIVT 
                   
               
               
                   
                   
                 gaggttttctttgctgttaaaggcgctgctgcctctttcg 
                   
                 EFKVRTQPAPGIAVQ 
                   
               
               
                   
                   
                 gtattgtcactgaatttaaggttagaactcagccagctc 
                   
                 YSYTFNLGSSAEKA 
                   
               
               
                   
                   
                 caggtatagcagtccaatattcctacaccttcaacttgg 
                   
                 QFIKDWQSFVSAKN 
                   
               
               
                   
                   
                 gttcgtctgctgagaaggctcaattcatcaaagattgg 
                   
                 LTRQFYTNMVIFDG 
                   
               
               
                   
                   
                 caatcatttgtctccgctaagaacttgaccagacaattc 
                   
                 DIILEGLFFGSKEQY 
                   
               
               
                   
                   
                 tacaccaatatggttattttcgatggtgatattatcctaga 
                   
                 EALRLEERFVPKNPG 
                   
               
               
                   
                   
                 aggtttgtttttcggttccaaggaacagtatgaagcatt 
                   
                 NILVLTDWLGMVGH 
                   
               
               
                   
                   
                 gcgtcttgaagagagatttgtgccaaaaaacccaggt 
                   
                 ALEDTILRLVGNTPT 
                   
               
               
                   
                   
                 aacatcttggttctaactgactggctaggtatggtcgga 
                   
                 WFYAKSLGFTPDTLI 
                   
               
               
                   
                   
                 catgccttggaagacacaatcttgagacttgttggtaat 
                   
                 PSSGIDEFFKYIENN 
                   
               
               
                   
                   
                 actcctacttggttttacgctaagtctctgggtttcacac 
                   
                 KAGTSTWFVTLSLE 
                   
               
               
                   
                   
                 cagatacgttgattccatcttctggaattgatgaatttttc 
                   
                 GGAINDVPADATAY 
                   
               
               
                   
                   
                 aagtacatagaaaacaataaggccggcacctccactt 
                   
                 GHRDVLFWVQIFMV 
                   
               
               
                   
                   
                 ggtttgttacattatcattggaaggcggtgctatcaacg 
                   
                 SPTGPVSSTTYDFAD 
                   
               
               
                   
                   
                 atgtacctgctgacgccaccgcttatggtcacagagat 
                   
                 GLYNVLTKAVPESE 
                   
               
               
                   
                   
                 gttttattctgggtccaaattttcatggtttcaccaactgg 
                   
                 GHAYLGCPDPKMA 
                   
               
               
                   
                   
                 tccagtttcttctaccacctatgacttcgctgatggtttat 
                   
                 NAQQKYWRQNLPR 
                   
               
               
                   
                   
                 acaatgtcttgactaaagctgtacccgagagtgaagg 
                   
                 LEELKETLDPKDTFH 
                   
               
               
                   
                   
                 ccatgcttacttgggttgtccagaccctaagatggctaa 
                   
                 NPQGILPA 
                   
               
               
                   
                   
                 tgcacaacaaaagtactggagacaaaacctaccaag 
                   
                   
                   
               
               
                   
                   
                 acttgaagaattgaaagaaaccctagaccccaaggat 
                   
                   
                   
               
               
                   
                   
                 acttttcacaacccacaaggtatcctaccagcc 
                   
                   
                   
               
               
                   
               
               
                 t807943 
                 Library 
                 atgaatccctcaattccatcctctagtatgggcaacact 
                 61 
                 MNPSIPSSSMGNTTS 
                 131 
               
               
                   
                   
                 acctcgatagccggtagagactgtctagtgtctgcact 
                   
                 IAGRDCLVSALGGN 
                   
               
               
                   
                   
                 tggaggtaacgctggtttagttgctttccaaaaccagc 
                   
                 AGLVAFQNQPLYQT 
                   
               
               
                   
                   
                 cactgtatcaaacaactgctgtacacgaatacaatttga 
                   
                 TAVHEYNLNTPVTP 
                   
               
               
                   
                   
                 atacccctgttacgccagccgctatcacttacccagag 
                   
                 AAITYPETAEHIAAV 
                   
               
               
                   
                   
                 acagctgaacatattgctgccgtcgttaaatgcgcaag 
                   
                 VKCASQYDYKVQA 
                   
               
               
                   
                   
                 ccaatatgattacaaggtccaagctcgttctggtggtca 
                   
                 RSGGHSFGNYGLGG 
                   
               
               
                   
                   
                 ctcctttggtaactacggtttgggtggtaccgatggag 
                   
                 TDGAVVVDMKYFN 
                   
               
               
                   
                   
                 ctgtcgttgttgacatgaagtatttcaaccaattttctatg 
                   
                 QFSMDDQTYEAVIG 
                   
               
               
                   
                   
                 gatgaccaaacctacgaagctgttatcggtcctggtac 
                   
                 PGTTLGDVDVELYN 
                   
               
               
                   
                   
                 tactttgggtgatgtggatgtagaattgtataacaacgg 
                   
                 NGKRAMAHGVCPTI 
                   
               
               
                   
                   
                 taaaagagccatggctcatggtgtctgtccaactatttc 
                   
                 STGGHFTMGGLGPT 
                   
               
               
                   
                   
                 caccggcggtcacttcacaatgggcggtttaggtcca 
                   
                 ARQWGLALDHVEE 
                   
               
               
                   
                   
                 actgctagacaatggggtttggctcttgaccacgtcga 
                   
                 VEVVLANSSIVRASN 
                   
               
               
                   
                   
                 agaagttgaggtggttctagcaaatagttctatcgtcag 
                   
                 TQNQEVFFAVKGAA 
                   
               
               
                   
                   
                 ggcctcgaatactcagaatcaagaagttttctttgcagt 
                   
                 ASFGIVTEFKVRTQP 
                   
               
               
                   
                   
                 aaagggagctgctgcttcttttggtatcgttaccgaattt 
                   
                 APGIAVQYSYTFNL 
                   
               
               
                   
                   
                 aaggtcagaacgcaaccagctccaggaattgctgttc 
                   
                 GSSAEKAQFIKDWQ 
                   
               
               
                   
                   
                 aatactcatacaccttcaacttgggttccagcgccgaa 
                   
                 SFVSAKNLTRQFYT 
                   
               
               
                   
                   
                 aaggctcagttcattaaggactggcaatctttcgtgtcc 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 gctaaaaacttaaccagacaattctacacaaacatggt 
                   
                 FGSKEQYEALGLEE 
                   
               
               
                   
                   
                 tatatttgacggtgatattatcttggaaggtctatttttcg 
                   
                 RFVPKNPGNILVLTD 
                   
               
               
                   
                   
                 gttccaaagagcaatatgaagctttgggtttggaagaa 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 agattcgtcccaaagaaccctggcaatatcctagtttta 
                   
                 RLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 acggattggttgggtatggtcggacatgccttagagg 
                   
                 LGFTPDTLIPSSGIDE 
                   
               
               
                   
                   
                 atacaatattgagattggttggtaacactcccacctggt 
                   
                 FFEYIENNKAGTST 
                   
               
               
                   
                   
                 tctacgccaagtcccttggttttactccagacacattgat 
                   
                 WFVTLSLEGGAIND 
                   
               
               
                   
                   
                 tccttcttctggtatcgatgaatttttcgaatatattgaaaa 
                   
                 VPADATAYGHRDVL 
                   
               
               
                   
                   
                 caataaggcaggtacttctacctggtttgtcaccctttca 
                   
                 FWVQIFMVSPTGPV 
                   
               
               
                   
                   
                 ttggaaggtggtgccattaacgacgtcccagctgatg 
                   
                 SSTTYDFADGLYNV 
                   
               
               
                   
                   
                 ctactgcatacggtcatcgtgacgtgctattctgggttc 
                   
                 LTKAVPESEGHAYL 
                   
               
               
                   
                   
                 agatatttatggtaagtcccactggcccagtaagttcca 
                   
                 GCPDPKMANAQQK 
                   
               
               
                   
                   
                 cgacttacgatttcgctgacggtttatataatgttctgact 
                   
                 YWRQNLPRLEELKE 
                   
               
               
                   
                   
                 aaagctgtgccagaatctgagggtcacgcctacttag 
                   
                 TLDPKDTFHNPQGIL 
                   
               
               
                   
                   
                 gatgtccagatccaaagatggctaatgcacaacaaaa 
                   
                 PA 
                   
               
               
                   
                   
                 atactggagacaaaacttgccaagattggaagaacta 
                   
                   
                   
               
               
                   
                   
                 aaggaaactttggacccaaaagataccttccataatcc 
                   
                   
                   
               
               
                   
                   
                 tcaaggcatccttcccgcc 
                   
                   
                   
               
               
                   
               
               
                 t807945 
                 Library 
                 atgggtaatactacctcaatagccggcagagattgcct 
                 62 
                 MGNTTSIAGRDCLV 
                 132 
               
               
                   
                   
                 agtctccgctttgggaggtaacgcaggtctggtggctt 
                   
                 SALGGNAGLVAFQN 
                   
               
               
                   
                   
                 ttcaaaaccagcctttgtatcaaacgacagctgtacac 
                   
                 QPLYQTTAVHEYNL 
                   
               
               
                   
                   
                 gaatacaatcttaacattcccgtcactccagccgctatc 
                   
                 NIPVTPAAITYPETA 
                   
               
               
                   
                   
                 acctacccagagactgctgaacaaatcgccgcagttg 
                   
                 EQIAAVVKCASQYD 
                   
               
               
                   
                   
                 ttaaatgtgcttcgcaatacgactataaggttcaagcta 
                   
                 YKVQARSGGHSFGN 
                   
               
               
                   
                   
                 ggtctggtggtcattccttcggtaactacggattaggc 
                   
                 YGLGGTDGAVVVD 
                   
               
               
                   
                   
                 ggtacagacggtgccgtcgttgttgatttgaagtacttc 
                   
                 LKYFNQFSMDDQTY 
                   
               
               
                   
                   
                 aatcagttttctatggatgaccaaacctatgaagctgtc 
                   
                 EAVIGPGTTLGDVD 
                   
               
               
                   
                   
                 attggtccaggtactaccttgggtgatgtagacgttgaa 
                   
                 VELYNNGKRAMAH 
                   
               
               
                   
                   
                 ttatataacaacggtaagcgtgctatggcccacggtgt 
                   
                 GVCPTISTGGHFTM 
                   
               
               
                   
                   
                 atgtccaactattagcacgggtggtcatttcactatggg 
                   
                 GGLGPTARQWGLAL 
                   
               
               
                   
                   
                 tggtcttggacctacggctagacaatggggtttagcctt 
                   
                 DHVEEVEVVLANSSI 
                   
               
               
                   
                   
                 ggatcacgtcgaagaagttgaggtcgttttggctaact 
                   
                 VRASNTQNQEVFFA 
                   
               
               
                   
                   
                 ctagtatcgttagagctagcaatacccaaaatcaagaa 
                   
                 VKGAAASFGIVTEF 
                   
               
               
                   
                   
                 gtgtttttcgctgttaaaggcgcagccgcttcgttcggt 
                   
                 KVRTQPAPGIAVQY 
                   
               
               
                   
                   
                 attgtcactgaatttaaggttagaactcaaccagctcca 
                   
                 SYTFNLGSSAEKAQF 
                   
               
               
                   
                   
                 ggtattgctgttcaatactcttacaccttcaatttgggctc 
                   
                 IKDWQSFVSAKNLT 
                   
               
               
                   
                   
                 ttccgccgagaaggcacagtttataaaagactggcaa 
                   
                 RQFYTNMVIFDGDII 
                   
               
               
                   
                   
                 tcattcgtttctgctaagaacttgacaagacaattctata 
                   
                 LEGLFFGSKEQYEAL 
                   
               
               
                   
                   
                 ccaacatggtcatctttgacggtgatattatcctagaag 
                   
                 RLEERFVPKNPGNIL 
                   
               
               
                   
                   
                 gtctgtttttcggtagtaaggaacaatacgaagctttgc 
                   
                 VLTDWLGMVGHAL 
                   
               
               
                   
                   
                 gtttagaagaaagattcgtgcccaagaaccctggtaa 
                   
                 EDTILRLVGNTPTWF 
                   
               
               
                   
                   
                 cattttggttttaactgattggctaggtatggtcggtcac 
                   
                 YAKSLGFTPDTLIPS 
                   
               
               
                   
                   
                 gctttggaggacacaatcctaagattggttggaaatac 
                   
                 SGIDEFFEYIENNKA 
                   
               
               
                   
                   
                 cccaacttggttctacgctaagtccttgggatttactcca 
                   
                 GTSTWFVTLSLEGG 
                   
               
               
                   
                   
                 gatactttgataccatcttccggtatcgacgaatttttcg 
                   
                 AINDVPADATAYGH 
                   
               
               
                   
                   
                 aatatattgaaaacaataaagccggtacctctacatggt 
                   
                 RDVLFWVQIFMVSP 
                   
               
               
                   
                   
                 tcgtaaccctttctcttgagggtggagccatcaacgac 
                   
                 TGPVSSTTYDFADG 
                   
               
               
                   
                   
                 gttccagctgatgctactgcatacggtcatagagatgt 
                   
                 LYNVLTKVVPESEG 
                   
               
               
                   
                   
                 cttgttttgggtacagattttcatggtcagccctacaggt 
                   
                 HAYLGCPDPKMAN 
                   
               
               
                   
                   
                 ccagtttcctctacgacctatgactttgctgatggtttata 
                   
                 AQQKYWRQNLPRL 
                   
               
               
                   
                   
                 caacgttttgactaaggtggttccagaatccgaaggcc 
                   
                 EELKETLDPKDTFH 
                   
               
               
                   
                   
                 acgcttacttaggttgtccagacccaaaaatggccaat 
                   
                 NPQGVLITEVGSATD 
                   
               
               
                   
                   
                 gctcaacaaaagtattggaggcaaaatttgccaagact 
                   
                 FWNLVEAIILISQLH 
                   
               
               
                   
                   
                 agaagaactaaaagaaacactggaccctaaggatact 
                   
                 ESVGQTYNMVPEM 
                   
               
               
                   
                   
                 tttcacaatccacaaggcgtcttgatcaccgaggttggt 
                   
                 GEQPVREMTKMFR 
                   
               
               
                   
                   
                 tccgccacggacttctggaacttagttgaagctattatc 
                   
                 MLEKTIQVSLEGLPY 
                   
               
               
                   
                   
                 ttaatctctcagttgcatgaatcagtcggccaaacatac 
                   
                 EEWLNRLQVENDD 
                   
               
               
                   
                   
                 aacatggtgcccgagatgggtgaacaacctgttagag 
                   
                 DPLRPLLPMFEEKV 
                   
               
               
                   
                   
                 aaatgactaagatgttccgtatgttggaaaagactattc 
                   
                 YDGRCQWEMYENM 
                   
               
               
                   
                   
                 aagtcagcttggaaggtcttccatacgaggaatggttg 
                   
                 PISDTENLRQYLQDV 
                   
               
               
                   
                   
                 aacagactgcaagtggaaaacgatgatgatccactga 
                   
                 PELATCPFLDQDIFK 
                   
               
               
                   
                   
                 ggccactgttgccaatgtttgaagaaaaagtctacgac 
                   
                 KFLSSLGLA 
                   
               
               
                   
                   
                 ggtagatgccaatgggaaatgtacgagaacatgccta 
                   
                   
                   
               
               
                   
                   
                 tttcggacaccgaaaacttgagacaatacttgcaagat 
                   
                   
                   
               
               
                   
                   
                 gttcctgaattagcaacttgtccattcttggatcaagata 
                   
                   
                   
               
               
                   
                   
                 tatttaagaagttcctttcctctcttggtttggca 
                   
                   
                   
               
               
                   
               
               
                 t807950 
                 Library 
                 atgggcaatacaacttcgatagctggtagagactgcct 
                 64 
                 MGNTTSIAGRDCLIS 
                 134 
               
               
                   
                   
                 tatttcagcactgggtggaaacagcgccttagctgcttt 
                   
                 ALGGNSALAAFPNE 
                   
               
               
                   
                   
                 tcccaacgagctattgtggacggccgatgtccatgaat 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                   
                 acaatttgaacttgccagtgactcctgctgctatcacct 
                   
                 PVTPAAITYPETAEQ 
                   
               
               
                   
                   
                 atccagaaaccgctgaacaaattgcaggagtagttaa 
                   
                 IAGVVKCASDYDYK 
                   
               
               
                   
                   
                 atgtgcctctgactacgattacaaggtccaggctcgttc 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 cggtggtcacagtttcggtaactatggtttaggtggtgc 
                   
                 LGGADGAVVVDMK 
                   
               
               
                   
                   
                 agatggtgctgttgtcgttgacatgaagcacttcactca 
                   
                 HFTQFSMDDETYEA 
                   
               
               
                   
                   
                 attttctatggacgatgaaacctacgaagctgttatcgg 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 tccaggcactacattgaatgatgttgacattgaattatat 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 aacaacggtaagagagccatggctcatggtgtgtgtc 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 ctaccatcaaaacaggtggtcacttcactattggcggtt 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 tgggtccaactgctagacaatggggtttagctttggatc 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 acgtcgaggaagtcgaagttgttttggccaactcttcc 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 attgtcagggcatctaatacccaaaaccaagacgtgtt 
                   
                 AANFGIVTEFKVRTE 
                   
               
               
                   
                   
                 tttcgctgttaagggcgccgctgctaacttcggaatcgt 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 taccgaatttaaggtcagaactgaaccagcaccaggtt 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 tggccgtccagtactcgtatactttcaatttgggtagtac 
                   
                 QSFISAKNLTRQFYN 
                   
               
               
                   
                   
                 cgccgaaaaagctcaatttgttaaggactggcaatcttt 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 catttccgctaagaatcttactagacaattttacaataac 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 atggtaatcttcgatggtgatatcattttggaaggtttgtt 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 ctttggttccaaagaacaatacgatgctctgggtcttga 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 agatcatttcgctccaaagaaccctggtaacatattggt 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 cctaaccgactggctaggtatggttggtcatgccttag 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 aagacaccatcttgaagcttgttggtaatacaccaactt 
                   
                 EFFEYIANHTAGTPA 
                   
               
               
                   
                   
                 ggttctatgcaaaatctttgggctttcgtcaagatactct 
                   
                 WFVTLSLEGGAINDI 
                   
               
               
                   
                   
                 gatcccatcagctggcattgacgaatttttcgagtacat 
                   
                 AEDATAYAHRDVLF 
                   
               
               
                   
                   
                 cgctaaccacaccgctggtactccagcctggtttgtaa 
                   
                 WVQLFMVNPLGPIS 
                   
               
               
                   
                   
                 cgttgtctttagagggtggtgctattaacgatatcgccg 
                   
                 DTTYEFTDGLYDVL 
                   
               
               
                   
                   
                 aagatgctacggcttacgcccatagagatgttctattct 
                   
                 ARAVPESVGHAYLG 
                   
               
               
                   
                   
                 gggtccaactgttcatggtcaaccctttgggtccaataa 
                   
                 CPDPRMEDAQQKY 
                   
               
               
                   
                   
                 gcgacacaacttacgaatttactgatggattatatgacg 
                   
                 WRTNLPRLQELKEE 
                   
               
               
                   
                   
                 tattggcaagagcagttcccgaatccgttggtcacgct 
                   
                 LDPKNTFHHPQGVM 
                   
               
               
                   
                   
                 tacttaggttgtccagatccaagaatggaagatgctca 
                   
                 PA 
                   
               
               
                   
                   
                 acaaaagtactggagaaccaacctgcctcgtttgcaa 
                   
                   
                   
               
               
                   
                   
                 gagcttaaagaagaattggacccaaagaatactttcca 
                   
                   
                   
               
               
                   
                   
                 tcacccacagggtgtcatgccagct 
                   
                   
                   
               
               
                   
               
               
                 t807955 
                 Library 
                 atgggtaatacgacatcaatcgcagccggaagagact 
                 65 
                 MGNTTSIAAGRDCL 
                 135 
               
               
                   
                   
                 gccttctgtcggctgtcggtggcaaccacgctcatgta 
                   
                 LSAVGGNHAHVAFQ 
                   
               
               
                   
                   
                 gcctttcaggatcaattattgtaccaagctactgctgtg 
                   
                 DQLLYQATAVEPYN 
                   
               
               
                   
                   
                 gagccatataacctaaacatacctgttacccccgccgc 
                   
                 LNIPVTPAAVTYPQS 
                   
               
               
                   
                   
                 tgttacttacccacaatctgctgaagaaattgcagctgt 
                   
                 AEEIAAVVQCASEY 
                   
               
               
                   
                   
                 cgttcaatgtgcttccgaatatggttacaaggttcaagc 
                   
                 GYKVQARSGGHSFG 
                   
               
               
                   
                   
                 tcgtagcggtggtcactccttcggtaattacggtttggg 
                   
                 NYGLGGEDGAIVVE 
                   
               
               
                   
                   
                 cggtgaagatggtgccatcgtcgttgaaatgaaacatt 
                   
                 MKHFNQFSMDESTN 
                   
               
               
                   
                   
                 tcaatcaattttctatggacgaatctaccaacattgctac 
                   
                 IATIGPGITLGDLDTA 
                   
               
               
                   
                   
                 tattggtccaggtatcaccttgggtgacttggatactgc 
                   
                 LYNAGYRAMAHGIC 
                   
               
               
                   
                   
                 tttatacaacgccggatatagagcaatggctcacggta 
                   
                 PTIRTGGHLTMGGL 
                   
               
               
                   
                   
                 tatgtccaacaatcagaacaggtggacatttgaccatg 
                   
                 GPTARQWGLALDH 
                   
               
               
                   
                   
                 ggtggtctaggtcctactgccaggcagtggggcttgg 
                   
                 VEEVEVVLANSSIVR 
                   
               
               
                   
                   
                 ccttggatcacgttgaggaagtcgaagttgtgttagcta 
                   
                 ASDTQNQDIFFAVK 
                   
               
               
                   
                   
                 actcttccattgttagagcttcagatactcaaaatcaag 
                   
                 GAAASFGIVTEFKVR 
                   
               
               
                   
                   
                 acattttcttcgctgtcaagggtgctgccgctagttttgg 
                   
                 TEQAPGLAVQYSFT 
                   
               
               
                   
                   
                 tattgttaccgaatttaaggtcagaactgaacaagctcc 
                   
                 FNLQTPAEKAKLVK 
                   
               
               
                   
                   
                 aggtcttgccgtacaatattctttcactttcaacttacaga 
                   
                 DWQAFIAQEDLTWK 
                   
               
               
                   
                   
                 ccccagcagaaaaagcaaagttggtaaaagactggc 
                   
                 FYSNMNIFDGQIILE 
                   
               
               
                   
                   
                 aagctttcatcgcccaagaggatttaacatggaagtttt 
                   
                 GIYFGSKAEYDALG 
                   
               
               
                   
                   
                 actcaaatatgaatattttcgatggtcaaatcattctgga 
                   
                 LEKRFPTSEPGTVLV 
                   
               
               
                   
                   
                 aggaatctacttcggttccaaggctgaatatgacgctct 
                   
                 LTDWLGMVGHGLE 
                   
               
               
                   
                   
                 aggtttggagaagagatttcccacttctgaaccaggta 
                   
                 DVILRLVGNTPTWF 
                   
               
               
                   
                   
                 ccgtcttggtcttgacagattggctaggtatggtcggtc 
                   
                 YAKSLGFTPRALIPD 
                   
               
               
                   
                   
                 acggcttagaagatgttatattgcgtttagttggtaacac 
                   
                 SAIDDFFNYIHKNNP 
                   
               
               
                   
                   
                 cccaacttggttttacgccaaaagtttgggcttcacgcc 
                   
                 GTVSWFVTLSLEGG 
                   
               
               
                   
                   
                 aagagctttgatcccagactctgctattgatgactttttc 
                   
                 AINKVPEDATAYGH 
                   
               
               
                   
                   
                 aactatatccacaagaataaccctggtactgttagttgg 
                   
                 RDVLFWVQIFMINPL 
                   
               
               
                   
                   
                 ttcgttactttgtctcttgaaggtggtgctataaataaagt 
                   
                 GPVSQTTYGFADGL 
                   
               
               
                   
                   
                 cccagaagacgctaccgcctacggtcatagagatgta 
                   
                 YDVLAKAVPESAGH 
                   
               
               
                   
                   
                 ttgttttgggttcagatatttatgattaacccattaggccc 
                   
                 AYLGCPDPRMPNAQ 
                   
               
               
                   
                   
                 cgtcagccaaactacatacggtttcgctgacggtttgta 
                   
                 QAYWRSNLPRLEEL 
                   
               
               
                   
                   
                 cgatgttttggctaaggcagttccagagtccgcaggtc 
                   
                 KGELDPKDVFHNPQ 
                   
               
               
                   
                   
                 atgcttacttgggctgtcctgacccaaggatgccaaac 
                   
                 GVMVVS 
                   
               
               
                   
                   
                 gcccaacaagcatactggagatccaacctacctagat 
                   
                   
                   
               
               
                   
                   
                 tggaagaactgaagggtgaattggatccaaaagacgt 
                   
                   
                   
               
               
                   
                   
                 ttttcataaccctcaaggtgtaatggtcgtcagc 
                   
                   
                   
               
               
                   
               
               
                 t807965 
                 Library 
                 atgggtaacacgacctctatcgctgccggacgtgact 
                 66 
                 MGNTTSIAAGRDCLI 
                 136 
               
               
                   
                   
                 gtctgatttcggcagtcggtgctgctaatgttgccttcc 
                   
                 SAVGAANVAFQDQL 
                   
               
               
                   
                   
                 aggatcaattattgtaccaagctacagctgtacaacctt 
                   
                 LYQATAVQPYNLNI 
                   
               
               
                   
                   
                 ataacctaaacataccagttactccagccgctgttacct 
                   
                 PVTPAAVTYPQSAD 
                   
               
               
                   
                   
                 acccacaaagcgcagacgaaattgctgccgtggtca 
                   
                 EIAAVVKCASEYGY 
                   
               
               
                   
                   
                 agtgcgcttcagagtatggctacaaagttcaagctagg 
                   
                 KVQARSGGHSFGNY 
                   
               
               
                   
                   
                 tccggtggtcactcctttggtaattacggtcttggtggc 
                   
                 GLGGQDGAIVIEMK 
                   
               
               
                   
                   
                 caagatggtgccatcgttattgaaatgaagcatttctct 
                   
                 HFSQFSMDESTFIATI 
                   
               
               
                   
                   
                 cagttttctatggatgaatcaaccttcatcgctactatag 
                   
                 GPGITLGDLDTDLY 
                   
               
               
                   
                   
                 gtcccggtattactttgggtgacttggatactgacttgta 
                   
                 NAGHRAMAHGICPT 
                   
               
               
                   
                   
                 caacgccggacacagagctatggctcatggtatctgt 
                   
                 IRTGGHLTVGGLGPT 
                   
               
               
                   
                   
                 ccaactattagaacgggtggtcacttaacagtcggtgg 
                   
                 ARQWGLALDHVEE 
                   
               
               
                   
                   
                 actaggtcctaccgctagacaatggggtttggcattgg 
                   
                 VEVVLANSSIVRASD 
                   
               
               
                   
                   
                 atcacgtagaagaagtcgaggtggttttagccaacag 
                   
                 TQNQDLFFAIKGAA 
                   
               
               
                   
                   
                 ctccattgtcagagcttctgacactcaaaatcaagattt 
                   
                 ASFGIVTEFKVRTEQ 
                   
               
               
                   
                   
                 gttctttgctattaagggtgcagctgcctctttcggaatc 
                   
                 APGMAVQYSYTFHL 
                   
               
               
                   
                   
                 gttaccgaatttaaagtcagaactgaacaagctccagg 
                   
                 GTSAEKAKFVKDW 
                   
               
               
                   
                   
                 tatggctgtccaatatagttacactttccatctgggcaca 
                   
                 QAFIAQENLTWKFY 
                   
               
               
                   
                   
                 tccgcagaaaaggctaagttcgttaaagattggcagg 
                   
                 TNLVIFDDQIILEGIY 
                   
               
               
                   
                   
                 cattcatcgctcaagagaacttaacttggaagttttatac 
                   
                 FGTKEEYDSLGLEQ 
                   
               
               
                   
                   
                 caatttggttattttcgacgatcaaatcatactagaaggt 
                   
                 RFPPTDAGTVLILTD 
                   
               
               
                   
                   
                 atctactttggtacgaaggaagaatacgatagtttaggt 
                   
                 WLAMIGHGLEDTIL 
                   
               
               
                   
                   
                 ttggaacaacgtttcccacctacggacgccggcactg 
                   
                 KLVGDTPTWFYAKS 
                   
               
               
                   
                   
                 ttttgattttaaccgattggctagctatgatcggtcatggt 
                   
                 LGFTPRALIPDSAIDE 
                   
               
               
                   
                   
                 ttggaggacaccatcttgaagctagtcggtgatacacc 
                   
                 FFDYIHENNPGTLA 
                   
               
               
                   
                   
                 aacctggttttacgccaagtctttaggttttaccccaaga 
                   
                 WFVTLSLEGGAINA 
                   
               
               
                   
                   
                 gctttgattcccgacagtgctatcgacgaatttttcgatt 
                   
                 VPEDATAYGHRDVL 
                   
               
               
                   
                   
                 atatccacgaaaataacccaggaactttggcatggttc 
                   
                 FWFQLFVINPLGPIS 
                   
               
               
                   
                   
                 gttactttgtctttggaaggtggtgccattaacgctgtcc 
                   
                 QTTYGFADGLYDVL 
                   
               
               
                   
                   
                 ctgaagatgctacagcttacggtcatagagatgtgcttt 
                   
                 AQAVPESVSHAYMG 
                   
               
               
                   
                   
                 tttggttccaacttttcgtgattaacccattgggtccaatt 
                   
                 CPDPRLPNAQYAYW 
                   
               
               
                   
                   
                 tcccaaaccacatacggatttgctgacggtctttatgat 
                   
                 RSNLPKLEELKGILD 
                   
               
               
                   
                   
                 gttttggcccaagctgtcccagaatctgtgagccacgc 
                   
                 PEDIFHNPQGVVPS 
                   
               
               
                   
                   
                 atatatgggttgtccagaccctagattgccaaatgccc 
                   
                   
                   
               
               
                   
                   
                 aatacgcttattggcgttccaatttaccaaagttggagg 
                   
                   
                   
               
               
                   
                   
                 aattaaaaggtatattagacccagaagacatctttcaca 
                   
                   
                   
               
               
                   
                   
                 acccacagggtgttgttccttca 
                   
                   
                   
               
               
                   
               
               
                 t807974 
                 Library 
                 atgttatcaacaatggccttctcttttgttttgagaattttgt 
                 67 
                 MLSTMAFSFVLRILS 
                 137 
               
               
                   
                   
                 cccctctattcttgatactacagcttagcacggctgcttc 
                   
                 PLFLILQLSTAASTST 
                   
               
               
                   
                   
                 gaccagtactttgcgtcaatgcttgctgaccgcagtcc 
                   
                 LRQCLLTAVQNDPT 
                   
               
               
                   
                   
                 aaaacgatccaactttagtagctgtggacggtgatttgt 
                   
                 LVAVDGDLLYQTLA 
                   
               
               
                   
                   
                 tgtatcaaactttagccgttcaagtttacaatcttaactg 
                   
                 VQVYNLNWPVTPA 
                   
               
               
                   
                   
                 gccagtcacacccgctgctgttgcatttccaaaatctac 
                   
                 AVAFPKSTQQVASIV 
                   
               
               
                   
                   
                 ccaacaagttgcttctatcgtaaattgtgccgcttcccta 
                   
                 NCAASLGYKVQAKS 
                   
               
               
                   
                   
                 ggctacaaggtccaagccaagtctggaggtcactcct 
                   
                 GGHSYGNYGLGGT 
                   
               
               
                   
                   
                 acggtaactatggtctgggtggtactaacggtgctatta 
                   
                 NGAISINLKNMKSFS 
                   
               
               
                   
                   
                 gcatcaacttaaagaatatgaaatcattctctatgaatta 
                   
                 MNYTNYQATVGAG 
                   
               
               
                   
                   
                 caccaactaccaggctacagtcggtgccggtatgttg 
                   
                 MLNGELDEYLHNA 
                   
               
               
                   
                   
                 aatggcgaattggacgagtatttacataacgctggtgg 
                   
                 GGRAVAHGTSPQIG 
                   
               
               
                   
                   
                 tagggccgttgctcacggaacctctccacaaattggtg 
                   
                 VGGHATIGGLGPSA 
                   
               
               
                   
                   
                 tcggtggtcatgctactatcggtggattgggtccatctg 
                   
                 RQYGMELDHVLEAE 
                   
               
               
                   
                   
                 caagacaatacggtatggaacttgaccacgttttggaa 
                   
                 VVLANGTVVRASST 
                   
               
               
                   
                   
                 gctgaagttgttctggctaacggcacggtagtcagag 
                   
                 QNSDLLFAIKGAGA 
                   
               
               
                   
                   
                 caagttcaactcaaaactcagatttgttgttcgccattaa 
                   
                 SFGVVTEFVFRTEPE 
                   
               
               
                   
                   
                 gggtgctggtgccagctttggtgttgtcactgagttcgt 
                   
                 PGSAVQYTFTFGLGS 
                   
               
               
                   
                   
                 ctttagaacagaacctgaaccaggtagtgctgtgcagt 
                   
                 TSARADLFKKWQSF 
                   
               
               
                   
                   
                 ataccttcacttttggtttaggctccacgtctgctagagc 
                   
                 ISQPDLTRKFASICTL 
                   
               
               
                   
                   
                 agatttgttcaagaaatggcaatccttcatatcccaacc 
                   
                 LDHVLVISGTFFGTK 
                   
               
               
                   
                   
                 agacttgactcgtaagtttgcctctatctgtacgctattg 
                   
                 EEYDALGLEDQFPG 
                   
               
               
                   
                   
                 gatcatgtacttgtaattagcggtacctttttcggtactaa 
                   
                 HTNSTVIVFTDWLG 
                   
               
               
                   
                   
                 ggaagaatacgacgctttgggacttgaagatcaattcc 
                   
                 LVAQWAEQSILDLT 
                   
               
               
                   
                   
                 ccggtcacactaattcgaccgttatcgtgtttaccgatt 
                   
                 GDIPADFYARCLSFT 
                   
               
               
                   
                   
                 ggttaggcttggttgctcaatgggctgagcaatctatct 
                   
                 EKTLIPSNGVDQLFE 
                   
               
               
                   
                   
                 tggacttgactggcgacattccagctgatttctacgcca 
                   
                 YLDSADTGALLWFV 
                   
               
               
                   
                   
                 gatgtctgtcctttaccgaaaaaaccctgattccttctaa 
                   
                 IFDLEGGAINDVPMD 
                   
               
               
                   
                   
                 cggtgtcgaccagttattcgaatatttggatagtgcaga 
                   
                 ATGYAHRDTLFWLQ 
                   
               
               
                   
                   
                 cactggtgctttattatggttcgtcattttcgacttggaag 
                   
                 SYAITLGSVSETTYD 
                   
               
               
                   
                   
                 gtggtgctattaacgatgttccaatggatgctactggtt 
                   
                 FLDSVNEIIRNNTPG 
                   
               
               
                   
                   
                 acgcacacagagataccttgttttggctacaatcatac 
                   
                 LGNGVYPGYVDPRL 
                   
               
               
                   
                   
                 gctatcacattgggttctgtttccgaaaccacttatgattt 
                   
                 ENAREAYWGSNLPR 
                   
               
               
                   
                   
                 cttagattctgttaacgaaatcataagaaataatacccct 
                   
                 LMQIKSLYDPTDLFH 
                   
               
               
                   
                   
                 ggtttgggtaatggtgtttaccctggttacgtcgaccca 
                   
                 NPQGVLPA 
                   
               
               
                   
                   
                 agattagaaaacgctagagaagcttattggggttctaa 
                   
                   
                   
               
               
                   
                   
                 tttgccacgtttgatgcaaataaagtctttgtatgaccca 
                   
                   
                   
               
               
                   
                   
                 acagacttgtttcataacccacaaggtgtactaccagc 
                   
                   
                   
               
               
                   
                   
                 c 
                   
                   
                   
               
               
                   
               
               
                 t807980 
                 Library 
                 atgggcaataccacatccattgccggacgtgattgcct 
                 68 
                 MGNTTSIAGRDCLIS 
                 138 
               
               
                   
                   
                 gatcagtgcattgggtggtaactcggctttagctgtcttt 
                   
                 ALGGNSALAVFPNE 
                   
               
               
                   
                   
                 cctaacgaattgctatggacggctgacgtgcatgagta 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                   
                 taatttgaaccttcccgttactccagctgccataacttac 
                   
                 PVTPAAITYPETAAQ 
                   
               
               
                   
                   
                 ccagaaaccgctgctcagattgcaggagttgtcaagt 
                   
                 IAGVVKCASDYDYK 
                   
               
               
                   
                   
                 gtgccagcgattacgactataaagttcaagctagatca 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 ggtggtcactctttcggtaactacggtttaggtggtgca 
                   
                 LGGADGAVVVDMK 
                   
               
               
                   
                   
                 gatggagctgtagttgttgacatgaagcacttcactca 
                   
                 HFTQFSMDDETYEA 
                   
               
               
                   
                   
                 attttctatggatgacgaaacttacgaagctgtcatcgg 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 tccaggtaccacattgaatgacgttgatattgaattgta 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 caacaatggtaaaagggccatggctcatggtgtctgtc 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 ctaccatcaagactggtggccacttcaccattggtggtt 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 taggcccaactgccagacaatggggtctggctttagat 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 catgttgaagaggtagaagtcgtgttggctaactcttcc 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 atagtcagagcctctaatacacaaaaccaagatgtctt 
                   
                 AANFGIVTEFKVRTE 
                   
               
               
                   
                   
                 ctttgctgttaagggtgcagctgcaaacttcggtattgtt 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 accgaatttaaggtgagaactgaaccagctccaggttt 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 ggctgttcaatattcgtacactttcaatttgggttctaccg 
                   
                 QSFISAKNLTRQFYN 
                   
               
               
                   
                   
                 ccgaaaaagctcagttcgtcaaggactggcaatccttt 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 atctccgcaaagaacttgacgcgtcaattctataataac 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 atggttatctttgacggagacattatccttgagggtttgtt 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 tttcggttcaaaggaacaatacgatgccctaggtttaga 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 agatcacttcgctccaaagaaccccggcaacatcttg 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 gttcttactgactggttaggtatggtaggtcacgctttgg 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 aagatactattttgaaactggttggtaacacaccaacat 
                   
                 EFFEYIANHTAGTPA 
                   
               
               
                   
                   
                 ggttctacgctaagtctttgggttttagacaagatacctt 
                   
                 WFVTLSLEGGAIND 
                   
               
               
                   
                   
                 gattccttcggctggcatagacgagttcttcgaatatat 
                   
                 VAEDATAYAHRDV 
                   
               
               
                   
                   
                 cgctaaccataccgcaggtactcctgcctggtttgtga 
                   
                 LFWVQLFMVNPVGP 
                   
               
               
                   
                   
                 cccttagtttggaaggaggtgctattaacgacgtcgct 
                   
                 ISDTTYEFTDGLYDV 
                   
               
               
                   
                   
                 gaagatgctactgcttacgcacacagagatgttctattc 
                   
                 LARAVPESVGHAYL 
                   
               
               
                   
                   
                 tgggttcaattatttatggttaatccagtcggtccaatctc 
                   
                 GCPDPRMEDAQQK 
                   
               
               
                   
                   
                 tgacactacctatgaatttactgatggcttgtacgatgtg 
                   
                 YWRTNLPRLQELKE 
                   
               
               
                   
                   
                 ctagctagagctgttccagaatccgtcggtcatgcttac 
                   
                 ELDPKNTFHHPQGV 
                   
               
               
                   
                   
                 ttgggttgtccagatcccaggatggaagacgctcaac 
                   
                 MPA 
                   
               
               
                   
                   
                 aaaagtactggagaacaaatttaccaagattgcaaga 
                   
                   
                   
               
               
                   
                   
                 attaaaagaagagcttgacccaaaaaacactttccatc 
                   
                   
                   
               
               
                   
                   
                 accctcagggagttatgccagcc 
                   
                   
                   
               
               
                   
               
               
                 t808013 
                 Library 
                 atgagatctcagttactacacggacttattggtctggttg 
                 69 
                 MRSQLLHGLIGLVA 
                 139 
               
               
                   
                   
                 ccttggtgtcaccttccttcgcagtccccacgaaacgt 
                   
                 LVSPSFAVPTKREAV 
                   
               
               
                   
                   
                 gaagctgtaacctcttgcttgacaaatgctaaggtccc 
                   
                 TSCLTNAKVPIDAK 
                   
               
               
                   
                   
                 aatagacgctaagggttcgcaaacttggacccaagat 
                   
                 GSQTWTQDGTAYN 
                   
               
               
                   
                   
                 ggtacagcctataacttgaggttacaatttgagccaatc 
                   
                 LRLQFEPIAIAVPTTV 
                   
               
               
                   
                   
                 gctattgccgttccaactactgttgctcaaatcagcgca 
                   
                 AQISAAVACGSKHG 
                   
               
               
                   
                   
                 gctgtcgcctgtggttctaagcatggcgtttccgtcagt 
                   
                 VSVSGKSGGHSYTS 
                   
               
               
                   
                   
                 ggtaaatctggtggtcactcctacacttctttgggtttgg 
                   
                 LGLGGEDGHLVIEL 
                   
               
               
                   
                   
                 gcggtgaagatggtcatcttgttattgaattggacagac 
                   
                 DRLYSVKLAKDGTA 
                   
               
               
                   
                   
                 tgtactcagtcaagttggctaaggatggaaccgctaag 
                   
                 KIQPGARLGHVATE 
                   
               
               
                   
                   
                 atccaaccaggtgctagattaggtcacgttgctactga 
                   
                 LYNQGKRALSHGTC 
                   
               
               
                   
                   
                 gttgtataaccagggtaaaagagcacttagtcatggta 
                   
                 TGVGLGGHALHGG 
                   
               
               
                   
                   
                 cctgtactggtgtaggtttgggtggtcacgctctacac 
                   
                 YGMVSRKHGLTLDS 
                   
               
               
                   
                   
                 ggcggatacggtatggtttccagaaagcatggtttaac 
                   
                 IIGATVVLYDGKVV 
                   
               
               
                   
                   
                 cttggactctataattggtgctactgtcgtcttgtacgac 
                   
                 HCSKTERSDLFWAIR 
                   
               
               
                   
                   
                 ggaaaagttgttcactgtagtaagacagaacgttccga 
                   
                 GAGASFGIVAELEFN 
                   
               
               
                   
                   
                 tttattctgggccattagaggtgcaggcgcttcttttggt 
                   
                 TFPAPEQMTYFDIGL 
                   
               
               
                   
                   
                 atcgtggctgaattagaatttaacaccttcccagcccct 
                   
                 NWDQNTAAQGLWE 
                   
               
               
                   
                   
                 gaacaaatgacctacttcgatattggtttgaattgggac 
                   
                 FQEFGKTMPSEITMQ 
                   
               
               
                   
                   
                 caaaacactgccgctcaaggtttgtgggaatttcaaga 
                   
                 IAIRKDGYSIDGAYI 
                   
               
               
                   
                   
                 atttggtaaaaccatgccttcagaaatcacgatgcaaat 
                   
                 GDEAGLRKALQPLL 
                   
               
               
                   
                   
                 tgctatacgtaaggatggatattctatcgatggtgcttac 
                   
                 SKLNVQVSASTVSW 
                   
               
               
                   
                   
                 atcggtgacgaagccggtttaagaaaggcacttcaac 
                   
                 MGLVTHFAGTAEIN 
                   
               
               
                   
                   
                 cattgttgagcaagttaaatgttcaagtctcggcttcga 
                   
                 PTSASYDAHDTFYA 
                   
               
               
                   
                   
                 ctgtgagctggatgggtctggttacacatttcgccggt 
                   
                 TSLTTRELSLEQFKS 
                   
               
               
                   
                   
                 actgctgagattaacccaacttctgcttcctatgatgca 
                   
                 FVNSISTTGKSSSHS 
                   
               
               
                   
                   
                 cacgacactttctacgctacttctttgacaaccagagaa 
                   
                 WWVQMDIQGGKYS 
                   
               
               
                   
                   
                 ttgtcattagaacaattcaagtcattcgtaaactccatca 
                   
                 AVAKPKPTDMAYV 
                   
               
               
                   
                   
                 gtaccaccggtaagtcaagttctcattcttggtgggtcc 
                   
                 HRDALLLFQFYDSV 
                   
               
               
                   
                   
                 agatggacattcagggtggcaaatactctgccgttgct 
                   
                 PQGQTYPSDGFSLLT 
                   
               
               
                   
                   
                 aagccaaaaccaacggatatggcttatgttcatagaga 
                   
                 TLRQSISKSLRAGTW 
                   
               
               
                   
                   
                 tgctttgcttttgtttcaattctacgattcagtgccccaag 
                   
                 GMYANYPDSQLKA 
                   
               
               
                   
                   
                 gtcaaacctacccatctgacggtttctccttactaactac 
                   
                 DRAAEMYWGSNLP 
                   
               
               
                   
                   
                 tctgagacaatccatttctaaatctcttagagccggcac 
                   
                 RLQKIKAAYDPKNIF 
                   
               
               
                   
                   
                 atggggtatgtatgcaaattacccagactcccaattga 
                   
                 RNPQSVKPKA 
                   
               
               
                   
                   
                 aggctgaccgtgctgctgaaatgtactggggtagcaa 
                   
                   
                   
               
               
                   
                   
                 cctgcctagactacagaagattaaggctgcctatgatc 
                   
                   
                   
               
               
                   
                   
                 ccaagaatatctttagaaatccacaaagtgttaagccta 
                   
                   
                   
               
               
                   
                   
                 aggcc 
                   
                   
                   
               
               
                   
               
               
                 t808014 
                 Library 
                 atgggaaacaccacatcaacttctgctggtcaatgtct 
                 70 
                 MGNTTSTSAGQCLL 
                 140 
               
               
                   
                   
                 attgtccgccgtgggtggcaatccagcattggtcgcttt 
                   
                 SAVGGNPALVAFQN 
                   
               
               
                   
                   
                 tcagaacgctcctttataccaagccgttgatgtaagac 
                   
                 APLYQAVDVRPYNL 
                   
               
               
                   
                   
                 cctataatctggacgttccagttactccagtcgctgttac 
                   
                 DVPVTPVAVTTPET 
                   
               
               
                   
                   
                 cacgccagaaactgtcgatcaagttgctagtatagtca 
                   
                 VDQVASIVKCAADA 
                   
               
               
                   
                   
                 aatgcgctgccgacgctggttacaaggttcaacctaa 
                   
                 GYKVQPKSGGHSYG 
                   
               
               
                   
                   
                 gtctggtggtcactcctacggtaactatggtttgggag 
                   
                 NYGLGGVDGEVVV 
                   
               
               
                   
                   
                 gtgtagacggtgaggttgtcgtcgatttaaaaaatttcc 
                   
                 DLKNFQQFSMNNET 
                   
               
               
                   
                   
                 aacaattctctatgaacaacgaaacctggagggctact 
                   
                 WRATIGAGTLLGDV 
                   
               
               
                   
                   
                 attggtgcaggtacattgcttggtgacgtgaccactcgt 
                   
                 TTRLYNAGGRAMA 
                   
               
               
                   
                   
                 ttgtacaacgccggtggcagagctatggcacatggta 
                   
                 HGTCPQVGIGGHATI 
                   
               
               
                   
                   
                 cctgtccacaagttggcatcggaggtcacgccactatt 
                   
                 GGLGPTSRLWGAAL 
                   
               
               
                   
                   
                 ggtggtttaggtccaacgtcgagattgtggggtgctgc 
                   
                 DHIEEVQVVLANSSI 
                   
               
               
                   
                   
                 cctagatcatatcgaagaagtgcaggttgttcttgctaa 
                   
                 VRASQTENPDLLFA 
                   
               
               
                   
                   
                 tagctctattgttagagcttcacaaactgagaaccctga 
                   
                 LKGAGASFGIITEFT 
                   
               
               
                   
                   
                 cttgttatttgctttgaagggtgctggtgcctccttcggt 
                   
                 VRTEPAPGEAVQYS 
                   
               
               
                   
                   
                 atcataacagaatttactgtccgtaccgaaccagctcc 
                   
                 YTFNFGDNASKAKT 
                   
               
               
                   
                   
                 aggcgaagcagttcaatattcatacaccttcaactttgg 
                   
                 FKDWQAFVSTPNLN 
                   
               
               
                   
                   
                 tgataatgcttccaaggctaagactttcaaagattggca 
                   
                 RKFAATMTVLEDAI 
                   
               
               
                   
                   
                 agccttcgtgtctacaccaaatttgaacagaaagttcg 
                   
                 VASGTFFGTKEEFD 
                   
               
               
                   
                   
                 ccgctaccatgactgtactggaagacgcaattgttgctt 
                   
                 AFELESHFPENQGSN 
                   
               
               
                   
                   
                 ctggtaccttctttggaactaaggaagaatttgatgcttt 
                   
                 VTVVQDWLGLVAD 
                   
               
               
                   
                   
                 cgaattggagtctcactttcctgaaaatcaaggttccaa 
                   
                 WAEDAALEGGGGV 
                   
               
               
                   
                   
                 cgtcacggtcgttcaggattggctgggtttagtcgctg 
                   
                 PSAFYAKSLNFSPDT 
                   
               
               
                   
                   
                 actgggcagaagatgcagctttggaaggaggtggtg 
                   
                 LIPNDTIDDMFDYFS 
                   
               
               
                   
                   
                 gtgtcccatccgctttctatgccaaaagtttgaatttcag 
                   
                 TTEKDALLWFAIFDL 
                   
               
               
                   
                   
                 tccagatactcttatccccaacgacacgattgatgacat 
                   
                 SGGAVSDVPVHSTS 
                   
               
               
                   
                   
                 gttcgactacttttctaccacagaaaaggatgctttgttg 
                   
                 YTHRDTLFWLQSYA 
                   
               
               
                   
                   
                 tggttcgccatttttgacctttcgggtggtgctgtgtctg 
                   
                 ISVGPVSNTTIQFLD 
                   
               
               
                   
                   
                 atgtccccgttcactcaacttcttacactcatagagatac 
                   
                 GLSNLLTSSQPEVHF 
                   
               
               
                   
                   
                 tctgttttggttacaatcgtacgcaatatctgttggccca 
                   
                 GAYPGYVDPKLPDG 
                   
               
               
                   
                   
                 gtaagcaacactactatccaattcttggacggtttgtcta 
                   
                 QLAYWGSNLPKLEQ 
                   
               
               
                   
                   
                 atttgctaacctcttcacaacccgaagttcactttggtgc 
                   
                 IKAEVDPNDVFHNP 
                   
               
               
                   
                   
                 ttatccaggttacgttgacccaaaattgccagacggac 
                   
                 QSVKPAKQ 
                   
               
               
                   
                   
                 aattagcttattggggttccaacttgccaaagctagagc 
                   
                   
                   
               
               
                   
                   
                 aaatcaaggccgaagtagatcctaacgacgtgttccat 
                   
                   
                   
               
               
                   
                   
                 aacccacaatccgttaaaccagctaagcaa 
                   
                   
                   
               
               
                   
               
               
                 t808021 
                 Library 
                 atggctcagccaccttcctcagcattcgccacctgtcta 
                 71 
                 MAQPPSSAFATCLN 
                 141 
               
               
                   
                   
                 aatgatgtctgcggaggtcgtagtggctgtgtgggtta 
                   
                 DVCGGRSGCVGYPS 
                   
               
               
                   
                   
                 cccatcggacattttgtatcaaatcaactgggtagatag 
                   
                 DILYQINWVDRYNL 
                   
               
               
                   
                   
                 gtacaacttagacataaacttggagccagctgctgtta 
                   
                 DINLEPAAVTKPEIT 
                   
               
               
                   
                   
                 caaaaccagaaattacggaagatgtcgccgcttttatc 
                   
                 EDVAAFIKCASENN 
                   
               
               
                   
                   
                 aagtgtgctagcgaaaataacgtcaaggtacaagcca 
                   
                 VKVQARSGGHSYA 
                   
               
               
                   
                   
                 gatctggtggtcattcttacgctaatcacggtctgggtg 
                   
                 NHGLGGEDGALVID 
                   
               
               
                   
                   
                 gcgaagacggtgcattggttatcgatttagagaacttc 
                   
                 LENFQHFSMNWDN 
                   
               
               
                   
                   
                 caacacttttccatgaattgggacaactggcaagctac 
                   
                 WQATIGAGHKLHD 
                   
               
               
                   
                   
                 tattggagccggccataagcttcacgacgttactgaaa 
                   
                 VTEKLHDNGGRAIS 
                   
               
               
                   
                   
                 aactacatgataacggtggtagagctatctcacacggt 
                   
                 HGTCPGVGLGGHAT 
                   
               
               
                   
                   
                 acctgtcctggtgttggattgggtggtcatgctactattg 
                   
                 IGGLGPSSRMWGSC 
                   
               
               
                   
                   
                 gtggtttgggtccctcttctcgtatgtggggttcctgttta 
                   
                 LDHVVEVEVVTADG 
                   
               
               
                   
                   
                 gatcacgtcgttgaagtcgaagttgttactgctgacggt 
                   
                 KIQRASEDENSDLFF 
                   
               
               
                   
                   
                 aagattcaaagagcctctgaagatgaaaattcggactt 
                   
                 ALKGAGASFGIITEF 
                   
               
               
                   
                   
                 gttcttcgcactgaagggtgctggtgcttcatttggtata 
                   
                 VMRTNEEPGDVVEY 
                   
               
               
                   
                   
                 atcaccgaatttgtgatgagaacaaacgaagagccag 
                   
                 TFSLTFSRHRDLSPV 
                   
               
               
                   
                   
                 gcgatgttgtcgaatatacgttctctttgaccttctccag 
                   
                 FEAWQNLISDPDLD 
                   
               
               
                   
                   
                 acacagagacttgtccccagtttttgaagcttggcaaa 
                   
                 RRFGSEFVMHELGAI 
                   
               
               
                   
                   
                 acttgataagtgatccagatttagacagaagattcggtt 
                   
                 ITGTFFGTEEEFEAT 
                   
               
               
                   
                   
                 ccgagttcgttatgcatgaactaggtgctattatcactg 
                   
                 GIPDRIPTGKKSIVV 
                   
               
               
                   
                   
                 gtacctttttcggaactgaagaagaatttgaagcaactg 
                   
                 NDWLGSVAQQAQD 
                   
               
               
                   
                   
                 gtattcctgatcgtattccaaccggtaaaaagtctatcgt 
                   
                 AALWLSDLSTAFTA 
                   
               
               
                   
                   
                 tgtcaacgactggttgggttctgtcgctcaacaggccc 
                   
                 KSLAFTKDQLLSSES 
                   
               
               
                   
                   
                 aagatgccgctctttggctgagcgacttaagcaccgc 
                   
                 IMDLMDYIDDANRG 
                   
               
               
                   
                   
                 cttcactgctaaatctttggctttcaccaaggatcaattg 
                   
                 TLIWFLIFDVTGGRI 
                   
               
               
                   
                   
                 ttatcgtctgaaagtattatggaccttatggattacatcg 
                   
                 NDVPMNATAYRHR 
                   
               
               
                   
                   
                 atgacgctaacagaggtacattgatctggtttttgatctt 
                   
                 DKVMFCQGYGIGIP 
                   
               
               
                   
                   
                 cgatgtgactggaggtagaattaatgatgtacccatga 
                   
                 TLNGRTREFIEGINSL 
                   
               
               
                   
                   
                 acgccaccgcctataggcacagagacaaggttatgtt 
                   
                 IRSSVPTNLSTYAGY 
                   
               
               
                   
                   
                 ctgccaaggttacggcataggtatcccaactttgaacg 
                   
                 VDASLESPQDSYWG 
                   
               
               
                   
                   
                 gtaggacaagagagtttattgagggtataaattccttga 
                   
                 PNLDALGQVKEDW 
                   
               
               
                   
                   
                 tcagaagttctgtgcctaccaatttgtccacttacgctg 
                   
                 DPSDLFSNPQSVRPG 
                   
               
               
                   
                   
                 gttacgtcgatgcatctttagaatctccacaggactcct 
                   
                 QKSVVDYFDNRASS 
                   
               
               
                   
                   
                 attggggtccaaacctagacgctttgggacaagttaaa 
                   
                 NGSEDSSGGSNGGT 
                   
               
               
                   
                   
                 gaagactgggacccatccgatctgttttcaaatccaca 
                   
                 RDEQGGCWSWRRS 
                   
               
               
                   
                   
                 atctgttagacccggtcaaaagtccgtagttgattatttc 
                   
                 GPAFAVFVALFVGF 
                   
               
               
                   
                   
                 gataacagagcttcgtctaatggttcagaagacagctc 
                   
                 PTPQTSWVQKQNLR 
                   
               
               
                   
                   
                 tggtggcagtaatggaggtacccgtgatgaacaaggt 
                   
                 DPALDLTDAESPSRT 
                   
               
               
                   
                   
                 ggttgttggtcttggagaagatccggtccagcatttgct 
                   
                 PVVNPNTLTTDTMA 
                   
               
               
                   
                   
                 gtctttgttgctttattcgtaggtttccctactccacaaact 
                   
                 KLSRGAPGGKLKMT 
                   
               
               
                   
                   
                 tcttgggtccaaaagcagaacttgcgtgacccagcttt 
                   
                 LGLPVGAVMNCAD 
                   
               
               
                   
                   
                 agatctgacagacgccgaatcaccttccagaacacct 
                   
                 NSGARNLYIISVKGI 
                   
               
               
                   
                   
                 gttgttaacccaaacacgttaacaactgacaccatggc 
                   
                 GARLNRLPAGGVGD 
                   
               
               
                   
                   
                 caagttgtctcgtggcgctccaggtggtaaattaaaga 
                   
                 MVMATVKKGKPEL 
                   
               
               
                   
                   
                 tgactttgggtttgcccgtcggtgccgttatgaactgcg 
                   
                 RKKVHPAVIVRQSK 
                   
               
               
                   
                   
                 ctgacaattcgggtgcaagaaacctttacattatttcgg 
                   
                 PWKRFDGVFLYFED 
                   
               
               
                   
                   
                 tcaaaggaatcggtgctagattgaacagactaccagc 
                   
                 NAGVIVNPKGEMKG 
                   
               
               
                   
                   
                 tggtggtgttggtgatatggttatggctactgttaagaa 
                   
                 SAITGPVGKEAAEL 
                   
               
               
                   
                   
                 gggtaaaccagagttgagaaagaaggttcatccagc 
                   
                 WPRIASNSGVVM 
                   
               
               
                   
                   
                 cgtcatagtcagacaaagtaagccatggaaacgttttg 
                   
                   
                   
               
               
                   
                   
                 atggtgttttcttgtacttcgaagacaatgccggtgttatt 
                   
                   
                   
               
               
                   
                   
                 gtgaacccaaaaggagaaatgaagggaagcgctatc 
                   
                   
                   
               
               
                   
                   
                 actggtcctgttggtaaggaagctgccgaattgtggcc 
                   
                   
                   
               
               
                   
                   
                 aagaattgcttctaattcaggtgtcgtcatg 
                   
                   
                   
               
               
                   
               
               
                 t808022 
                 Library 
                 atgggaaattcggccagcgtggcaggtagagcttgttt 
                 72 
                 MGNSASVAGRACFV 
                 142 
               
               
                   
                   
                 tgtcgctgctgtaggtcatgatcccaacttggttacattc 
                   
                 AAVGHDPNLVTFRG 
                   
               
               
                   
                   
                 aggggtgacttactatatgagttccgtattcagccatca 
                   
                 DLLYEFRIQPSYNLA 
                   
               
               
                   
                   
                 tacaaccttgccataccagttcaccctacggtcgtcac 
                   
                 IPVHPTVVTYPKTTA 
                   
               
               
                   
                   
                 ctacccaaaaactaccgctcaagttgctgaaatcgtttc 
                   
                 QVAEIVSCAAAQNY 
                   
               
               
                   
                   
                 ttgcgccgctgcacaaaattataagatgcaagcctaca 
                   
                 KMQAYSGGHSYGN 
                   
               
               
                   
                   
                 gtggcggtcactcttacggtaactacggtttgggtgga 
                   
                 YGLGGEDGHVVVD 
                   
               
               
                   
                   
                 gaagatggtcatgttgttgtcgacttgaagaacttccaa 
                   
                 LKNFQDFTMDPDTH 
                   
               
               
                   
                   
                 gactttactatggatccagatactcacgttgctaccattg 
                   
                 VATIGAGTSLGDLQ 
                   
               
               
                   
                   
                 gcgctggtacttccttaggtgatctgcaagacagattgt 
                   
                 DRLWHAGGRAMAH 
                   
               
               
                   
                   
                 ggcacgctggtggtagagcaatggcccatggtagttg 
                   
                 GSCPQVGVGGHFTI 
                   
               
               
                   
                   
                 tcctcaagtgggtgtcggtggtcacttcaccatcggtg 
                   
                 GGLGMMSRQWGMS 
                   
               
               
                   
                   
                 gcttgggcatgatgtccagacagtggggtatgtctctg 
                   
                 LDHVVEAQVVLANS 
                   
               
               
                   
                   
                 gaccatgtcgttgaagctcaagtagtcttggccaattct 
                   
                 SVVTASDTQNQDIF 
                   
               
               
                   
                   
                 tctgtggttacggcttccgatactcaaaaccaagatattt 
                   
                 WAIKGAAASFGIVT 
                   
               
               
                   
                   
                 tttgggccatcaagggtgctgctgcttcgtttggtattgt 
                   
                 KFKVRTHGVPKAAI 
                   
               
               
                   
                   
                 tacaaaattcaaggtaagaacacacggtgttccaaag 
                   
                 QYQYTFSQGDVLDK 
                   
               
               
                   
                   
                 gccgctatccaatatcagtacaccttctctcaaggtgac 
                   
                 VKLFMAWQNIVAKP 
                   
               
               
                   
                   
                 gtattagacaaagttaagttgtttatggcttggcaaaac 
                   
                 NLTRNFSTELTIFQD 
                   
               
               
                   
                   
                 attgtcgctaagccaaatttgactcgtaacttcagtactg 
                   
                 GIMIMGSFFGTRDEF 
                   
               
               
                   
                   
                 aattgaccatattccaagatggaatcatgattatgggta 
                   
                 HKFELENDLPLQGL 
                   
               
               
                   
                   
                 gctttttcggtactagagatgaatttcataagttcgagtt 
                   
                 GNVAYITNWLSLVA 
                   
               
               
                   
                   
                 agaaaatgatttaccccttcaaggccttggtaatgttgc 
                   
                 HTAEDYLLRLTGNV 
                   
               
               
                   
                   
                 atatatcaccaactggctatccttggttgctcataccgct 
                   
                 LTSFYAKSLSFTADE 
                   
               
               
                   
                   
                 gaagactacttgttgagactgacaggtaacgtcttgac 
                   
                 LFNEQGLVTLFTYL 
                   
               
               
                   
                   
                 ttctttttacgccaaatctctatcattcacggctgacgaat 
                   
                 DAAPKGTPTWWVIF 
                   
               
               
                   
                   
                 tgttcaacgagcaaggtcttgttactttgttcacttattta 
                   
                 DLEGGATNDVPVNA 
                   
               
               
                   
                   
                 gacgcagctccaaaaggcacacctacctggtgggtta 
                   
                 TSYAHRDAIMWMQ 
                   
               
               
                   
                   
                 tcttcgatttggaaggaggtgccactaacgatgtccca 
                   
                 SYAVAGFEPPGFIIK 
                   
               
               
                   
                   
                 gttaacgctacttcttacgcccacagagatgctataatg 
                   
                 RFLNRLHGVVIGNR 
                   
               
               
                   
                   
                 tggatgcaaagttacgccgtcgctggttttgaaccacc 
                   
                 APGAVRSYPGYVDP 
                   
               
               
                   
                   
                 aggttttattattaagagattcctaaacagattgcatggt 
                   
                 YLRNAQETYWGPNL 
                   
               
               
                   
                   
                 gttgtaatcggtaatcgtgcacctggtgctgtccgttcc 
                   
                 ARLQDIKTAVDPDD 
                   
               
               
                   
                   
                 tatcctggttatgtcgacccatacttaagaaatgcccag 
                   
                 VFHNPQSVKVNSLS 
                   
               
               
                   
                   
                 gaaacctactggggtccaaacttggctagattacaag 
                   
                 PPDPGSHDV 
                   
               
               
                   
                   
                 atattaagacagctgttgatccagatgacgtttttcacaa 
                   
                   
                   
               
               
                   
                   
                 tccacaatccgttaaggtgaatagtctttcgccaccag 
                   
                   
                   
               
               
                   
                   
                 accctggaagccatgatgtc 
                   
                   
                   
               
               
                   
               
               
                 t808024 
                 Library 
                 atgggtcaaacgccaagctctcctctagccgactgttt 
                 73 
                 MGQTPSSPLADCLN 
                 143 
               
               
                   
                   
                 aaatgcagtttgcaacggaagagataactgtgtggctt 
                   
                 AVCNGRDNCVAFPS 
                   
               
               
                   
                   
                 ttccatccgctccactgtatcagatctcttgggtcgaca 
                   
                 APLYQISWVDRYNL 
                   
               
               
                   
                   
                 ggtacaatttggatatagaagtagagcccattgctgtta 
                   
                 DIEVEPIAVTRPETA 
                   
               
               
                   
                   
                 ccagaccagaaactgccgaagacgtttcaggtttcgt 
                   
                 EDVSGFVKCAAAHN 
                   
               
               
                   
                   
                 caaatgtgctgccgctcacaacattaaggttcaagcaa 
                   
                 IKVQAKSGGHSYAN 
                   
               
               
                   
                   
                 agtccggcggtcattcttacgctaactatggtcttggtg 
                   
                 YGLGGEDGELVVDL 
                   
               
               
                   
                   
                 gtgaagatggtgaattggtcgttgatttgagaaatttcc 
                   
                 RNFQDFSIDTNTWQ 
                   
               
               
                   
                   
                 aagattttagtatcgatacaaacacttggcaagccacct 
                   
                 ATFGAGHKLDDVTE 
                   
               
               
                   
                   
                 tcggcgctggtcacaagttagacgacgtcactgaaaa 
                   
                 KLHKNGKRAISHGT 
                   
               
               
                   
                   
                 attgcataagaacggtaagcgtgctatttcacacggta 
                   
                 CPGVGIGGHATIGGL 
                   
               
               
                   
                   
                 cttgccctggtgtcggtatcggtggtcacgctaccattg 
                   
                 GPESRMWGSCLDHV 
                   
               
               
                   
                   
                 gcggattaggtcctgagtctcgtatgtggggttcgtgtt 
                   
                 IGVEVVTADGSIVHA 
                   
               
               
                   
                   
                 tggatcatgtgatcggtgtagaagtcgttactgctgac 
                   
                 SDTENSDLFFALKG 
                   
               
               
                   
                   
                 ggaagcatagttcatgcctcggacaccgaaaattccg 
                   
                 AGASFGIVTSFVVKT 
                   
               
               
                   
                   
                 atttgttctttgctcttaaaggcgcaggagcttctttcggt 
                   
                 RPEPGSVVQYSYSV 
                   
               
               
                   
                   
                 attgtaacatcttttgttgttaagactagaccagaaccag 
                   
                 TFAKHADLSPVFRQ 
                   
               
               
                   
                   
                 gttccgttgtccaatacagctactctgtcacgttcgcaa 
                   
                 WQELVMDPGLDRR 
                   
               
               
                   
                   
                 aacacgctgacctatccccagttttcagacaatggcag 
                   
                 FGTEFTMHELGVIIS 
                   
               
               
                   
                   
                 gaattggtaatggatccaggtttggacagaagatttgg 
                   
                 GTFYGTDEEFQATGI 
                   
               
               
                   
                   
                 taccgaatttaccatgcacgagctgggtgtcattatctc 
                   
                 PDRIPKGKISVVFDD 
                   
               
               
                   
                   
                 tggtactttctatggtactgacgaagagttccaagccac 
                   
                 WMAVIAKHAEEAA 
                   
               
               
                   
                   
                 aggtattcctgatagaatcccaaagggtaagatttctgt 
                   
                 LSLSSISSAFTARSLA 
                   
               
               
                   
                   
                 tgttttcgatgattggatggctgttatagcaaaacacgc 
                   
                 FRREDKISPETITNL 
                   
               
               
                   
                   
                 cgaagaagctgctttgtcgttaagtagtatctcctctgct 
                   
                 MNYIDSADRGTLVW 
                   
               
               
                   
                   
                 tttaccgcccgttccttggctttcagaagagaagacaa 
                   
                 FLIFDATGGAISDVP 
                   
               
               
                   
                   
                 gatctcaccagaaactatcaccaacctgatgaactaca 
                   
                 TNATAYSHRDKVM 
                   
               
               
                   
                   
                 ttgattctgctgatagaggtactttggtctggttcctaatc 
                   
                 YCQGYGVGIPTLNQ 
                   
               
               
                   
                   
                 tttgatgctaccggtggtgccatttccgatgtcccaaca 
                   
                 QTKDFLSGIINTIQSG 
                   
               
               
                   
                   
                 aacgccacagcttactcacatagagacaaggttatgta 
                   
                 AGNTLTTYPGYVDP 
                   
               
               
                   
                   
                 ctgtcaaggctacggcgtaggtatacccactttaaatc 
                   
                 ALTNPQESYWGPNI 
                   
               
               
                   
                   
                 aacagaccaaggacttcttgtcgggtattattaacacta 
                   
                 DTLRAIKSQWDPNDI 
                   
               
               
                   
                   
                 tacaatctggtgccggtaatactttgactacttatcctgg 
                   
                 FHNPQSVRPAAVAA 
                   
               
               
                   
                   
                 ttatgtcgatccagctttgaccaacccacaagaatccta 
                   
                   
                   
               
               
                   
                   
                 ctggggaccaaacatcgacactttaagagctatcaag 
                   
                   
                   
               
               
                   
                   
                 agtcagtgggatccaaacgatatctttcataatccacaa 
                   
                   
                   
               
               
                   
                   
                 tctgttaggccagctgccgtggctgcc 
                   
                   
                   
               
               
                   
               
               
                 t808026 
                 Library 
                 atgcttaaaaccatcgctgccgttgtattcatttgctcgc 
                 74 
                 MLKTIAAVVFICSQA 
                 144 
               
               
                   
                   
                 aggcttttttggtccgtgcagacctaaagtccgagctg 
                   
                 FLVRADLKSELTAL 
                   
               
               
                   
                   
                 actgctttgggcgtgggtgccgtcttccctggagattc 
                   
                 GVGAVFPGDSVYTS 
                   
               
               
                   
                   
                 agtttacacgagcgatgctaagccatataacttgagatt 
                   
                 DAKPYNLRFDFKPA 
                   
               
               
                   
                   
                 tgacttcaaaccagctgctataacttttcccaatacccc 
                   
                 AITFPNTPADVSQIV 
                   
               
               
                   
                   
                 agccgatgtctctcaaattgttcaaatcgccggtaagta 
                   
                 QIAGKYAHKVAPRG 
                   
               
               
                   
                   
                 cgcacacaaggttgcaccaagaggtggtggtcattcc 
                   
                 GGHSYISNGVGGMD 
                   
               
               
                   
                   
                 tacatttctaacggtgttggtggaatggacaatagtatc 
                   
                 NSIIADMSHFKSIVV 
                   
               
               
                   
                   
                 attgctgatatgtctcacttcaagtctattgtagtccatac 
                   
                 HTNNDTATIETGNR 
                   
               
               
                   
                   
                 aaacaatgacactgctaccatcgaaactggtaacaga 
                   
                 LGDIALALFQYGRG 
                   
               
               
                   
                   
                 ttaggcgatatagctttagctttgttccaatatggtaggg 
                   
                 MPHGACPYVGIGGH 
                   
               
               
                   
                   
                 gtatgcctcacggtgcttgtccatacgtaggtattggtg 
                   
                 ANFGGFGFISRSWGL 
                   
               
               
                   
                   
                 gccacgccaactttggtggtttcggtttcatctcaagat 
                   
                 TLDVVEAIDLVLAN 
                   
               
               
                   
                   
                 cctggggtttgaccctagatgttgtcgaagctattgacc 
                   
                 GTITTVSATQNPDLY 
                   
               
               
                   
                   
                 tggttttagcaaacggcactatcacgacagtctctgcta 
                   
                 WAMRGSGSSFGITT 
                   
               
               
                   
                   
                 ctcaaaacccagacttgtattgggccatgagaggtag 
                   
                 AIHVRTFSAPASGIIA 
                   
               
               
                   
                   
                 cggtagttcttttggaatcaccaccgctatccatgttag 
                   
                 LDTWYLNLEQAVR 
                   
               
               
                   
                   
                 aaccttctccgcaccagcttctggtattatcgctttggac 
                   
                 ALSSFQDFAHNTVT 
                   
               
               
                   
                   
                 acttggtacttgaatcttgaacaagctgttagagccttg 
                   
                 LPSYFGGEFVVNAG 
                   
               
               
                   
                   
                 agttcctttcaagatttcgctcacaatactgtgactttacc 
                   
                 PSPGLLSITFFSGFW 
                   
               
               
                   
                   
                 atcttattttggtggtgaatttgtcgttaacgccggtcctt 
                   
                 GPPNQYNSTLAPWK 
                   
               
               
                   
                   
                 ccccaggtttgttgtctattacattcttctcgggattttgg 
                   
                 NSMPFPPNTTSYSQG 
                   
               
               
                   
                   
                 ggtcctccaaatcagtacaactctacgctagcaccatg 
                   
                 NYIESLSARFGGAPL 
                   
               
               
                   
                   
                 gaaaaattccatgccattccccccaaacacaacttcat 
                   
                 DTSLGPDNTDTFYV 
                   
               
               
                   
                   
                 actcgcaaggtaactacatagaaagcttgtccgcccgt 
                   
                 KSLIVPQVTISDEGA 
                   
               
               
                   
                   
                 ttcggaggtgctcccttggatacctctctaggtccagat 
                   
                 QVGISDKAWRALFQ 
                   
               
               
                   
                   
                 aatactgacactttttacgtcaagtcattaatagtcccac 
                   
                 YLINEQPNLPVDWFI 
                   
               
               
                   
                   
                 aagttaccatttctgatgaaggtgctcaagtaggtatta 
                   
                 EVELWGGQNSAINA 
                   
               
               
                   
                   
                 gcgataaagcttggagagctctgttccaatatttgataa 
                   
                 VPQASTAFAYRDLL 
                   
               
               
                   
                   
                 acgagcagcctaacctgcctgttgattggttcatcgaa 
                   
                 WTLQMYSYTPNHQP 
                   
               
               
                   
                   
                 gttgaattatggggtggtcaaaatagtgccattaacgc 
                   
                 PYPDAGFAFNDGMA 
                   
               
               
                   
                   
                 cgtcccacaagcttctacagcttttgcttatagagacttg 
                   
                 NSIIHNMPNGWNYG 
                   
               
               
                   
                   
                 ttgtggactttgcaaatgtactcttacaccccaaaccat 
                   
                 AYTNYVDNRLDDW 
                   
               
               
                   
                   
                 caaccaccttacccagacgccggttttgcattcaatga 
                   
                 QRLYYANHYPALQA 
                   
               
               
                   
                   
                 cggcatggctaatagtatcattcataacatgccaaacg 
                   
                 LKSRYDPSDTFSFPT 
                   
               
               
                   
                   
                 gttggaattatggtgcttacactaattacgttgataaccg 
                   
                 SIELL 
                   
               
               
                   
                   
                 tttagacgattggcagagattgtactatgctaaccacta 
                   
                   
                   
               
               
                   
                   
                 ccccgctttgcaagccttgaagtctaggtatgacccta 
                   
                   
                   
               
               
                   
                   
                 gtgatacattttcgttcccaacttccattgaactttta 
                   
                   
                   
               
               
                   
               
               
                 t808029 
                 Library 
                 atgactaccaacggtatacaacccggccatgtcggta 
                 75 
                 MTTNGIQPGHVGNL 
                 145 
               
               
                   
                   
                 atttaacacaggaccaagaggctaaacttcaacaattg 
                   
                 TQDQEAKLQQLWSI 
                   
               
               
                   
                   
                 tggtcgattgtactaacgttgttagatgttaagtccttgc 
                   
                 VLTLLDVKSLQGGD 
                   
               
               
                   
                   
                 aaggtggagatacttctgcccagacccaaccagacc 
                   
                 TSAQTQPDQRPSTSL 
                   
               
               
                   
                   
                 aacgtccaagtactagcttgtctagggctgacaccgtt 
                   
                 SRADTVVSAHGQTA 
                   
               
               
                   
                   
                 gtgtcagcacacggtcaaactgcttttaccgaagatct 
                   
                 FTEDLSQVLRENGM 
                   
               
               
                   
                   
                 atcccaagttttgagagaaaacggtatgtctaatccag 
                   
                 SNPDIKSVRESLSNT 
                   
               
               
                   
                   
                 atatcaagtccgtcagagaatctctgtccaacacttcta 
                   
                 SIDELRSGLLYTAKH 
                   
               
               
                   
                   
                 tcgacgaattgagatccggtttattgtacacagccaaa 
                   
                 DSPDVLLLRFLRAR 
                   
               
               
                   
                   
                 cacgattcacctgatgtcttgcttctaagattcttaagag 
                   
                 KWDVGKAFGMMLR 
                   
               
               
                   
                   
                 ctcgtaagtgggacgttggtaaggctttcggtatgatgt 
                   
                 ALVWRKDQHVDDK 
                   
               
               
                   
                   
                 tgagagcattggtatggagaaaagatcaacatgttgac 
                   
                 VIANPELAALVTSQN 
                   
               
               
                   
                   
                 gacaaggttattgctaatccagagctggccgctttggt 
                   
                 TVDTHAAKECKDFL 
                   
               
               
                   
                   
                 cacttctcagaacaccgtcgatacacacgccgctaag 
                   
                 DQMRMGKCYMHGT 
                   
               
               
                   
                   
                 gaatgtaaggattttctggaccaaatgagaatgggtaa 
                   
                 DRDGRPVLVVRVRF 
                   
               
               
                   
                   
                 atgctatatgcatggtaccgatagggacggaagacct 
                   
                 HQPSKQSEAVINRFI 
                   
               
               
                   
                   
                 gttttagttgttagagtcagattccaccaaccatctaagc 
                   
                 LHTIETARLLLAPPQ 
                   
               
               
                   
                   
                 aaagtgaagccgtgattaaccgttttatcttgcacacga 
                   
                 ETVTIIFDMTGFGLS 
                   
               
               
                   
                   
                 tcgaaacagctagattgctattggctccaccacaagaa 
                   
                 NMEYAPVKFIIECFQ 
                   
               
               
                   
                   
                 actgtcactattattttcgacatatggaccggtttcggttt 
                   
                 ENYPESLGYMLIHN 
                   
               
               
                   
                   
                 gtctaatatggaatacgcccctgttaaatttattatagaa 
                   
                 APWVFSGIWKIIKG 
                   
               
               
                   
                   
                 tgtttccaagaaaactatccagaatcgttaggctacatg 
                   
                 WMDPVIVSKVNFTN 
                   
               
               
                   
                   
                 cttattcataatgctccctgggttttttccggtatctggaa 
                   
                 KVSDLEKFIAPEQIV 
                   
               
               
                   
                   
                 gatcatcaagggttggatggatccagtcatagtgtcta 
                   
                 KELKGKEDWTYEY 
                   
               
               
                   
                   
                 aagtgaacttcactaacaaggtttcggatttagaaaaat 
                   
                 VEPVAGENELMADT 
                   
               
               
                   
                   
                 tcatcgctccagagcaaattgtaaaggaactaaaggg 
                   
                 ETRDRIYAERLKIGE 
                   
               
               
                   
                   
                 taaggaggactggacctacgaatatgtcgaacccgta 
                   
                 ELLLRTSEWVSTSQR 
                   
               
               
                   
                   
                 gcaggcgagaacgaattgatggctgacactgaaacc 
                   
                 KDAAATTTAREQRS 
                   
               
               
                   
                   
                 agagataggatttacgcagaaagattgaagatcggtg 
                   
                 ETIESLRQNYWQLD 
                   
               
               
                   
                   
                 aagagttgttgttgagaaccagcgaatgggtttccactt 
                   
                 PYVRGRTFLDRTGV 
                   
               
               
                   
                   
                 cacagcgtaaggacgctgctgccacgactacagcta 
                   
                 VKPGGKIDFYPSPDL 
                   
               
               
                   
                   
                 gagaacagcgttctgaaaccatagaaagtttgagaca 
                   
                 EPSTAKMLEVEHFE 
                   
               
               
                   
                   
                 aaattattggcaactagacccttacgttagaggtagaa 
                   
                 RTQFDPYLFLLPHGA 
                   
               
               
                   
                   
                 cttttttggatagaactggtgttgtgaagcctggaggta 
                   
                 RIAVRHCSVTALPTY 
                   
               
               
                   
                   
                 agattgacttctacccatctccagatttggagccaagta 
                   
                 LKAHPRGMLSTMAF 
                   
               
               
                   
                   
                 ctgccaaaatgttagaagtcgaacactttgaaagaacc 
                   
                 SFLRVLSSLLLVLQL 
                   
               
               
                   
                   
                 caatttgatccataccttttcttattgccacacggtgcta 
                   
                 STAASTSTLRQCLLT 
                   
               
               
                   
                   
                 gaattgctgttaggcattgtagcgtcaccgctttaccaa 
                   
                 AVQNDPTLVAVDG 
                   
               
               
                   
                   
                 cctatcttaaggctcacccacgtggtatgctatctacaa 
                   
                 DLLFQTLAVQVYNL 
                   
               
               
                   
                   
                 tggccttcagtttcctacgtgtattgtcttccctattgctg 
                   
                 NWPVTPAAVAFPKS 
                   
               
               
                   
                   
                 gtcttgcaattatcaaccgctgctagtacttcgacgttg 
                   
                 AQQVSSIVNCAASL 
                   
               
               
                   
                   
                 agacaatgtcttttgactgctgttcaaaacgacccaacc 
                   
                 GYKVQAKSGGHSY 
                   
               
               
                   
                   
                 ctggttgccgttgatggagatttgcttttccaaaccttgg 
                   
                 GNYGLGGTNGAISIN 
                   
               
               
                   
                   
                 ctgttcaagtctacaacttgaactggccagtcactcctg 
                   
                 LKNMKSFSMNYTN 
                   
               
               
                   
                   
                 ctgctgtagcctttcccaaatccgcccagcaagtttctt 
                   
                 YQATVGAGMLNGE 
                   
               
               
                   
                   
                 ctatcgttaattgcgcagcatcccttggttataaagttca 
                   
                 LDDYLHNAGGRAIA 
                   
               
               
                   
                   
                 agctaagtcgggtggtcattcttacggtaactatggctt 
                   
                 HGTSPQIGVGGHATI 
                   
               
               
                   
                   
                 aggtggtacaaacggcgcaatctctataaaccttaaaa 
                   
                 GGLGPAARQYGME 
                   
               
               
                   
                   
                 atatgaagtcattctcaatgaattacactaactaccaag 
                   
                 LDHVLEAEVVLANG 
                   
               
               
                   
                   
                 ctacggttggtgctggtatgttgaatggagagttagac 
                   
                 TVVRASSTQNSDLLF 
                   
               
               
                   
                   
                 gattatctgcacaatgccggtggtagagcaattgctca 
                   
                 AIKGAGASFGVVTE 
                   
               
               
                   
                   
                 tggcacaagcccacaaattggtgtcggtggtcacgca 
                   
                 FVFRTEPEPGSAVQY 
                   
               
               
                   
                   
                 actatcggtggtttgggtcctgctgccagacagtacgg 
                   
                 SFTFGLGSTSSRADL 
                   
               
               
                   
                   
                 tatggaattagatcacgtcttggaagctgaagttgtgtt 
                   
                 FKKWQSFISQPDLTR 
                   
               
               
                   
                   
                 agcaaatggtacagtcgtcagagcttcctctacccaaa 
                   
                 KFASICTILDHVLVIS 
                   
               
               
                   
                   
                 actcggacttgttgtttgccatcaagggagctggtgctt 
                   
                 GTFFGTKAEYDALG 
                   
               
               
                   
                   
                 ctttcggtgtggtgactgaatttgtttttagaacagagcc 
                   
                 LEDQFPGHTNSTVIV 
                   
               
               
                   
                   
                 agaacctggatctgctgttcagtactccttcacttttggtt 
                   
                 FTDWLGLVAQWAE 
                   
               
               
                   
                   
                 taggctccacctcttcacgtgccgacctattcaagaag 
                   
                 QSILDLTGGIPADFY 
                   
               
               
                   
                   
                 tggcaatcattcatttctcaaccagacttgactagaaaa 
                   
                 SRCLSFTEKTPIPSTG 
                   
               
               
                   
                   
                 ttcgccagcatctgtaccatcttggaccatgttttggtca 
                   
                 VDQLFEYLDSADTG 
                   
               
               
                   
                   
                 tttccggtactttctttggtactaaagctgaatacgacgc 
                   
                 ALLWFVIFDLEGGAI 
                   
               
               
                   
                   
                 tttaggtttagaagatcaatttccaggtcacaccaattct 
                   
                 NDVPMDATGYAHR 
                   
               
               
                   
                   
                 actgtgatcgtatttaccgattggttgggactggttgctc 
                   
                 DTLFWLQSYAITLGS 
                   
               
               
                   
                   
                 aatgggctgaacaatctattttggatttgaccggtggta 
                   
                 VSQTTYDFLDRVNEI 
                   
               
               
                   
                   
                 ttccagccgatttctactccagatgtttatcttttactgaa 
                   
                 IRNNTPGLGNGVYP 
                   
               
               
                   
                   
                 aagactccaattccatcgactggtgtcgatcaattgttc 
                   
                 GYVDPRLQNAREAY 
                   
               
               
                   
                   
                 gagtatctggacagtgcagatacgggagctctattgtg 
                   
                 WGSNLPRLMQIKSL 
                   
               
               
                   
                   
                 gtttgttattttcgatttggagggtggtgccattaacgat 
                   
                 YDPSDLFHNPQGVL 
                   
               
               
                   
                   
                 gtcccaatggatgctacaggttacgctcatagagaca 
                   
                 PA 
                   
               
               
                   
                   
                 ccttgttttggttacagtcttatgccataactttaggttctg 
                   
                   
                   
               
               
                   
                   
                 tttcccaaactacctacgacttcctggatcgtgttaacg 
                   
                   
                   
               
               
                   
                   
                 aaataattagaaataacacaccaggtttgggaaacggt 
                   
                   
                   
               
               
                   
                   
                 gtttacccaggttacgtcgaccctagacttcagaatgc 
                   
                   
                   
               
               
                   
                   
                 aagagaagcttattggggttccaatttgccaagacttat 
                   
                   
                   
               
               
                   
                   
                 gcaaattaaaagcctttatgacccatcggacctgttcca 
                   
                   
                   
               
               
                   
                   
                 caacccccaaggtgttttgcctgct 
                   
                   
                   
               
               
                   
               
               
                 t808039 
                 Library 
                 atgggccagggtcaatcctctgccggtggtttgcaag 
                 76 
                 MGQGQSSAGGLQD 
                 146 
               
               
                   
                   
                 actgcttaacgtcagcagtgggtagcggaaatctagct 
                   
                 CLTSAVGSGNLAVP 
                   
               
               
                   
                   
                 gtaccttctaaacccttctaccaacaaactgatgtcaag 
                   
                 SKPFYQQTDVKPYN 
                   
               
               
                   
                   
                 ccatataacttggatatccacgtccatccagttgctgtta 
                   
                 LDIHVHPVAVTYPQ 
                   
               
               
                   
                   
                 catacccacaaactaacgaggacgttgctgctattgtc 
                   
                 TNEDVAAIVRCAKE 
                   
               
               
                   
                   
                 agatgtgctaaggaacacgaagccaaagtccagcca 
                   
                 HEAKVQPRSGGHSY 
                   
               
               
                   
                   
                 cgttccggtggtcattcgtacggtaattttgccaccggt 
                   
                 GNFATGNGNDNMIV 
                   
               
               
                   
                   
                 aacggaaacgataacatgatagttgttgacttgaagca 
                   
                 VDLKHFKQFSMDDN 
                   
               
               
                   
                   
                 cttcaagcaattctctatggatgacaatacctggatcgc 
                   
                 TWIATLGSGHLLGD 
                   
               
               
                   
                   
                 aactttaggttccggccaccttctgggtgatgtcacaaa 
                   
                 VTKKLLANGGRAM 
                   
               
               
                   
                   
                 gaaattgttagctaacggtggtagggctatggctcatg 
                   
                 AHGTCPQVGIGGHA 
                   
               
               
                   
                   
                 gtacttgtcctcaagttggtattggcggtcacgctacca 
                   
                 TIGGLGPMSRMWGS 
                   
               
               
                   
                   
                 ttggtggtctaggtccaatgtctaggatgtggggcagtt 
                   
                 SLDHVQEITVVLANS 
                   
               
               
                   
                   
                 ccttggaccacgttcaagaaatcactgtggtcttggcc 
                   
                 SIITASPTQNKDVFW 
                   
               
               
                   
                   
                 aattctagcattatcacggcctctccaacccaaaataa 
                   
                 AMKGAGASFGIITEF 
                   
               
               
                   
                   
                 ggatgttttttgggctatgaagggtgcaggagcctcatt 
                   
                 KVITHPAPGEAVKY 
                   
               
               
                   
                   
                 cggtataattactgaatttaaagttattacccatccagct 
                   
                 SFGFSGGSHRDQAK 
                   
               
               
                   
                   
                 ccaggtgaggctgttaagtatagtttcggtttttcggga 
                   
                 RFKKWQSMIADPGL 
                   
               
               
                   
                   
                 ggttcacacagagatcaagctaagagattcaaaaagt 
                   
                 SRKLASQVVLSEIG 
                   
               
               
                   
                   
                 ggcaatctatgatcgctgaccctggattgagtagaaaa 
                   
                 MIISGTFFGTQAEYN 
                   
               
               
                   
                   
                 cttgcttctcaagtagttctgagtgaaatcggtatgatta 
                   
                 QLNLTSVFPEMSSH 
                   
               
               
                   
                   
                 tatcaggtacctttttcggtacccaggctgaatacaacc 
                   
                 KIIVFNDWAGLVGH 
                   
               
               
                   
                   
                 aattgaacttaacttctgtcttccctgaaatgtcctcccat 
                   
                 WAEDVGLQLGGGIS 
                   
               
               
                   
                   
                 aagattatcgtatttaacgattgggctggtctagtgggt 
                   
                 SPFYSKSLAFTPNDLI 
                   
               
               
                   
                   
                 cactgggccgaagacgtgggtttacaattgggtggtg 
                   
                 PAEGIDRFFEYLDEV 
                   
               
               
                   
                   
                 gaatctcttctccattctactccaagagcttggctttcac 
                   
                 DKGTLIWFGIFDLEG 
                   
               
               
                   
                   
                 cccaaacgacttgattcctgctgaaggtattgacagatt 
                   
                 GATNDIPADATAYG 
                   
               
               
                   
                   
                 tttcgaatatttggatgaagttgataagggtactttgatct 
                   
                 HRDALFYFQSYGVN 
                   
               
               
                   
                   
                 ggtttggtatattcgatttggaaggtggcgccactaac 
                   
                 LGLKVKDETRDFIN 
                   
               
               
                   
                   
                 gatattccagcagacgcaactgcatacggtcatagag 
                   
                 GMNSVLEGSLSNHK 
                   
               
               
                   
                   
                 atgcattgttttatttccagtcatatggtgtcaatctagga 
                   
                 LGAYAGYVDPALSL 
                   
               
               
                   
                   
                 ttaaaggttaaggatgagacaagagactttatcaatgg 
                   
                 EAAQVGYWGDNLP 
                   
               
               
                   
                   
                 tatgaatagcgtccttgaaggttctttgagcaaccacaa 
                   
                 RLRQIKRAVDPDDV 
                   
               
               
                   
                   
                 actgggtgcttacgctggttacgttgatcccgctctttct 
                   
                 FHNLQSVRPAAS 
                   
               
               
                   
                   
                 ttggaagccgcccaggttggttactggggtgacaactt 
                   
                   
                   
               
               
                   
                   
                 accacgtctgagacaaattaagagagctgtagatcca 
                   
                   
                   
               
               
                   
                   
                 gacgacgttttccataatttgcaatccgtcagaccagct 
                   
                   
                   
               
               
                   
                   
                 gcttcc 
                   
                   
                   
               
               
                   
               
               
                 t808040 
                 Library 
                 atgggtaataagccatccactcctttagcccattgcttg 
                 77 
                 MGNKPSTPLAHCLR 
                 147 
               
               
                   
                   
                 agagatgtttgtgcaggaaggggtaactgtgtcgcttt 
                   
                 DVCAGRGNCVAFPN 
                   
               
               
                   
                   
                 cccaaacgagtatctttaccaggctaactgggtaaaac 
                   
                 EYLYQANWVKPYN 
                   
               
               
                   
                   
                 cctacaatttggacgtgccagttaagccaattgctgtct 
                   
                 LDVPVKPIAVFRPDN 
                   
               
               
                   
                   
                 ttagacctgataatgccgctgacgtcgctgctgctgtta 
                   
                 AADVAAAVKCAGQ 
                   
               
               
                   
                   
                 agtgtgccggtcaatcatcggttcacgttcaagcaaaa 
                   
                 SSVHVQAKSGGHSY 
                   
               
               
                   
                   
                 tctggtggccactcttatgcaaacttcggtctaggtggt 
                   
                 ANFGLGGGDGGLMI 
                   
               
               
                   
                   
                 ggtgatggtggtttgatgatcgacctgcaacatttgaac 
                   
                 DLQHLNKFSMNNET 
                   
               
               
                   
                   
                 aagtttagcatgaacaacgaaacctggcaagctacatt 
                   
                 WQATFGSGFLLGDL 
                   
               
               
                   
                   
                 cggatccggtttcctattgggcgatttagacaagcaac 
                   
                 DKQLHANGNRAMA 
                   
               
               
                   
                   
                 tgcacgctaatggtaatcgtgccatggctcatggtactt 
                   
                 HGTCPGVGIGGHATI 
                   
               
               
                   
                   
                 gcccaggtgttggcataggtggtcacgccaccatcgg 
                   
                 GGIGPSSRMWGTAL 
                   
               
               
                   
                   
                 aggtattggtccatcttccagaatgtggggtacggcttt 
                   
                 DHVLEVEVVTADGK 
                   
               
               
                   
                   
                 agatcacgtattggaagtcgaagttgtgactgctgatg 
                   
                 IQRASKTQNSDLFW 
                   
               
               
                   
                   
                 gtaaaattcaaagagccagtaagacccagaactctga 
                   
                 GLQGAGASFGIITEF 
                   
               
               
                   
                   
                 cttgttttggggtttgcaaggtgctggtgcttcattcggc 
                   
                 VVRTEPEPGSVVEY 
                   
               
               
                   
                   
                 atcataactgaatttgttgtccgtaccgaacctgaacca 
                   
                 AYSLNFGKQADMAP 
                   
               
               
                   
                   
                 ggttctgtcgttgagtacgcctactctctaaatttcggca 
                   
                 VYKKWQDLVGDPN 
                   
               
               
                   
                   
                 aacaagcagatatggctccagtgtataagaagtggca 
                   
                 LDRRFTSLFIAEPLG 
                   
               
               
                   
                   
                 agaccttgtgggtgaccctaacttagatagaagattca 
                   
                 VLITGTFYGTLDEYK 
                   
               
               
                   
                   
                 ccagtttgtttattgccgaaccattgggtgttttgatcact 
                   
                 ASGIPDKLPASGASI 
                   
               
               
                   
                   
                 ggtacattctacggtaccctagacgaatacaaggcttc 
                   
                 TVMDWLGSLAHIAE 
                   
               
               
                   
                   
                 cggaatcccagacaagttgcccgcttcgggtgcctcc 
                   
                 KTGLYLSNVSTKFV 
                   
               
               
                   
                   
                 attacagtcatggattggttgggtagcttagctcacatc 
                   
                 SRSLALREEDLLSEQ 
                   
               
               
                   
                   
                 gctgaaaaaactggtttatatttgtctaacgtatctacta 
                   
                 SIDDLFKYMGSADA 
                   
               
               
                   
                   
                 aatttgtttccagatcattagcattaagggaagaggacc 
                   
                 DTPLWFVIFDNEGG 
                   
               
               
                   
                   
                 ttttgagcgaacagtccattgatgatttgtttaagtacat 
                   
                 AIADVPDNSTAYPH 
                   
               
               
                   
                   
                 gggctctgctgacgctgacacaccattgtggttcgttat 
                   
                 RDKIILYQSYSVGLL 
                   
               
               
                   
                   
                 tttcgataacgaaggtggtgccatcgctgatgtccctg 
                   
                 GVSDKMINFVDGIQ 
                   
               
               
                   
                   
                 ataattctactgcttatccacatagagacaagattatact 
                   
                 DLVQKGAPNAHTTY 
                   
               
               
                   
                   
                 gtaccaaagttactccgttggtttgttgggagtttctgac 
                   
                 AGYINANLDRNAAQ 
                   
               
               
                   
                   
                 aagatgataaatttcgtcgatggtattcaagatcttgtac 
                   
                 KFYWGDKLPQLQQL 
                   
               
               
                   
                   
                 aaaagggcgctcctaacgcccacacgacttacgctg 
                   
                 KKKFDPTSLFSNPQS 
                   
               
               
                   
                   
                 gttatatcaacgctaacttagacagaaatgctgcccaa 
                   
                 IDPAD 
                   
               
               
                   
                   
                 aaattttattggggtgacaagttgccacagctgcaaca 
                   
                   
                   
               
               
                   
                   
                 actaaagaagaagttcgacccaacatcgttattcagca 
                   
                   
                   
               
               
                   
                   
                 atccacaatctattgatccagccgat 
                   
                   
                   
               
               
                   
               
               
                 t808041 
                 Library 
                 atgggtaacaccacttccatcgcagccggcagagatt 
                 78 
                 MGNTTSIAAGRDCL 
                 148 
               
               
                   
                   
                 gtttggtttcagctgtcggtccagctcatgtgacatttca 
                   
                 VSAVGPAHVTFQDA 
                   
               
               
                   
                   
                 agacgctctgctttatcagacgaccgccgttgatcctta 
                   
                 LLYQTTAVDPYNLN 
                   
               
               
                   
                   
                 caatttgaacattcccgtaactccagctgctgtcacata 
                   
                 IPVTPAAVTYPQSAE 
                   
               
               
                   
                   
                 cccacaatcggccgaagagatagctgctgttgtcaaa 
                   
                 EIAAVVKCASDYDY 
                   
               
               
                   
                   
                 tgcgcttccgactatgattacaaggttcaagcacgtagt 
                   
                 KVQARSGGHSFGNY 
                   
               
               
                   
                   
                 ggaggtcacagcttcggtaattacggtctaggtggtca 
                   
                 GLGGQNGAIVVDM 
                   
               
               
                   
                   
                 aaacggtgccatcgtcgttgacatgaagcacttctctc 
                   
                 KHFSQFSMDESTFV 
                   
               
               
                   
                   
                 aattttctatggatgaatctactttcgttgctaccattggt 
                   
                 ATIGPGTTLGDLDTE 
                   
               
               
                   
                   
                 ccaggtactacgttaggcgacttggataccgaactata 
                   
                 LYNAGGRAMAHGIC 
                   
               
               
                   
                   
                 taatgctggtggtagggccatggcccatggtatctgtc 
                   
                 PTIRTGGHLTVGGLG 
                   
               
               
                   
                   
                 ctactattagaactggcggtcacttaaccgtcggtgga 
                   
                 PTARQWGLALDHIE 
                   
               
               
                   
                   
                 ttgggtccaacagccagacagtggggtctggctttgg 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 atcatattgaagaggtagaagttgttttggctaactcttc 
                   
                 NTQNQDILFAVKGA 
                   
               
               
                   
                   
                 catcgtgagagcatcgaacactcaaaatcaagacattt 
                   
                 AASFGIVTEFKVRTQ 
                   
               
               
                   
                   
                 tattcgctgtaaagggtgcagctgcttcttttggtatagt 
                   
                 EAPGLAVQFSFTFNL 
                   
               
               
                   
                   
                 caccgaatttaaagttagaactcaagaagctccaggtt 
                   
                 GSPAQKAKLVKDW 
                   
               
               
                   
                   
                 tggctgttcaattctccttcaccttcaacttgggttctcct 
                   
                 QAFIAQENLSWKFY 
                   
               
               
                   
                   
                 gcacaaaaggctaagctagtcaaagattggcaagcat 
                   
                 SNLVIFDGQIILEGIF 
                   
               
               
                   
                   
                 ttattgctcaggaaaacttgagctggaagttctactcaa 
                   
                 FGSKEEYDELDLEK 
                   
               
               
                   
                   
                 acttggtcatcttcgacggtcaaataatcttagaaggta 
                   
                 RFPTSEPGTVLVLTD 
                   
               
               
                   
                   
                 ttttctttggatcgaaagaggaatacgacgaactagatt 
                   
                 WLGMIGHALEDTIL 
                   
               
               
                   
                   
                 tggaaaagagatttccaacgtcagagcccggcactgt 
                   
                 KLVGDTPTWFYAKS 
                   
               
               
                   
                   
                 tttggttttaacagattggctgggcatgatcggacacgc 
                   
                 LGFTPDTLIPDSAIDD 
                   
               
               
                   
                   
                 tttggaagatactattttgaagttggtgggtgacacccc 
                   
                 FFDYIHKTNAGTLA 
                   
               
               
                   
                   
                 aacgtggttttatgctaagtccctgggtttcactccaga 
                   
                 WFVTLSLEGGAINS 
                   
               
               
                   
                   
                 cactcttatcccagattctgccattgatgacttcttcgact 
                   
                 VSEDATAYGHRDVL 
                   
               
               
                   
                   
                 acatccacaagactaacgctggtaccttagcttggttc 
                   
                 FWFQVFVVNPLGPIS 
                   
               
               
                   
                   
                 gtaaccttgtcattggaaggtggtgcaattaattctgttt 
                   
                 QTTYDFTNGLYDVL 
                   
               
               
                   
                   
                 cggaagatgctacagcttatggtcatagagatgtcttgt 
                   
                 AQAVPESAGHAYLG 
                   
               
               
                   
                   
                 tttggtttcaagttttcgttgttaatcctttaggtcctattag 
                   
                 CPDPKMPDAQRAY 
                   
               
               
                   
                   
                 tcaaaccacgtacgatttcactaacggcctgtatgacg 
                   
                 WRSNLPRLEDLKGD 
                   
               
               
                   
                   
                 tccttgcccaagccgtaccagaatccgccggtcacgc 
                   
                 LDPKDTFHNPQGVQ 
                   
               
               
                   
                   
                 ttacctaggttgtccagaccctaaaatgccagacgctc 
                   
                 VGP 
                   
               
               
                   
                   
                 aacgtgcctactggcgtagtaacttgccaagacttgaa 
                   
                   
                   
               
               
                   
                   
                 gatttgaagggtgacttggacccaaaggatactttcca 
                   
                   
                   
               
               
                   
                   
                 taatccacagggtgttcaagttggtcca 
                   
                   
                   
               
               
                   
               
               
                 t808045 
                 Library 
                 atgctgtcaaccatggcattcagctttgtccttagaatttt 
                 79 
                 MLSTMAFSFVLRILS 
                 149 
               
               
                   
                   
                 atctccattgttcttgatcctacaattatctactgccgcta 
                   
                 PLFLILQLSTAASTST 
                   
               
               
                   
                   
                 gtacatccactttgaggcagtgtttgttaaccgctgttca 
                   
                 LRQCLLTAVQNDPT 
                   
               
               
                   
                   
                 aaatgaccctacgttggtagctgttgatggtgatttgct 
                   
                 LVAVDGDLLYQTLA 
                   
               
               
                   
                   
                 gtaccaaactcttgccgtgcaagtctataacttgaactg 
                   
                 VQVYNLNWPVTPA 
                   
               
               
                   
                   
                 gccagttacccccgctgctgtcgcctttccaaagtcga 
                   
                 AVAFPKSTQQVASIV 
                   
               
               
                   
                   
                 ctcaacaagttgcttctatagttaactgcgctgcatcctt 
                   
                 NCAASLGYKVQAKS 
                   
               
               
                   
                   
                 gggatacaaagtgcaagctaagtctggcggtcattcct 
                   
                 GGHSYGNYGLGGT 
                   
               
               
                   
                   
                 acggtaattatggtttgggtggtaccaatggtgccattt 
                   
                 NGAISINLKNMKSFS 
                   
               
               
                   
                   
                 caatcaacttaaagaacatgaaatcgttctctatgaact 
                   
                 MNYTNYQATVGAG 
                   
               
               
                   
                   
                 acacgaattaccaagccacagttggtgctggtatgctt 
                   
                 MLNGELDEYLHNA 
                   
               
               
                   
                   
                 aacggcgagttagacgaatatttgcacaacgctggtg 
                   
                 GGRAVAHGTSPQIG 
                   
               
               
                   
                   
                 gtcgtgctgtcgcacacggaacttcccctcagattggt 
                   
                 VGGHATIGGLGPSA 
                   
               
               
                   
                   
                 gtaggtggtcatgctactattggaggactaggtccatc 
                   
                 RQYGMELDHVLEAE 
                   
               
               
                   
                   
                 ggctagacaatacggtatggaattggatcacgtcttag 
                   
                 VVLANGTVVRASST 
                   
               
               
                   
                   
                 aagccgaagttgttttggcaaacggtaccgtagtccgt 
                   
                 QNSDLLFAIKGAGA 
                   
               
               
                   
                   
                 gcttcttctactcagaatagcgacttgctgttcgccatca 
                   
                 SFGVVTEFVFRTEPE 
                   
               
               
                   
                   
                 agggtgctggtgctagttttggtgtcgttacagagtttgt 
                   
                 PGSAVQYTFTFGLGS 
                   
               
               
                   
                   
                 gttcagaacagaaccagaaccaggttctgctgttcaat 
                   
                 TSARADLFKKWQSF 
                   
               
               
                   
                   
                 ataccttcactttcggcttgggttccacctctgccagag 
                   
                 ISQPDLTRKFASICTL 
                   
               
               
                   
                   
                 ccgatctatttaagaaatggcaatccttcatatcccaac 
                   
                 LDHVLVISGTFFGTK 
                   
               
               
                   
                   
                 cagacctgactagaaagtttgcaagtatctgtaccttgt 
                   
                 EEYDALGLEDQFPG 
                   
               
               
                   
                   
                 tagatcatgttttggtcatttctggtactttctttggtacaa 
                   
                 HTNSTVIVFTDWLG 
                   
               
               
                   
                   
                 aagaagaatacgacgctttgggcttggaagatcaattt 
                   
                 LVAQWAEQSILDLT 
                   
               
               
                   
                   
                 cccggacacactaactctactgttatcgttttcaccgatt 
                   
                 GGIPADFYARCLSFT 
                   
               
               
                   
                   
                 ggttgggtttggtggctcaatgggctgaacaatcaattt 
                   
                 EKTLIPSNGVDQLFE 
                   
               
               
                   
                   
                 tagacctgactggtggtatcccagctgatttctacgcaa 
                   
                 YLDSADTGALLWFV 
                   
               
               
                   
                   
                 gatgtttgagctttactgaaaagaccctaattccttccaa 
                   
                 IFDLEGGAINDVPMD 
                   
               
               
                   
                   
                 tggtgtcgaccaattattcgagtacctagactcagcag 
                   
                 ATGYAHRDTLFWLQ 
                   
               
               
                   
                   
                 atactggtgctttgttatggttcgtcatctttgatcttgaa 
                   
                 SYAITLGSVSETTYD 
                   
               
               
                   
                   
                 ggtggtgccattaacgacgtcccaatggacgctaccg 
                   
                 FLDNVNEIIRNNTPG 
                   
               
               
                   
                   
                 gctatgctcacagagataccttgttttggctacagtctta 
                   
                 LGNGVYPGYVDPRL 
                   
               
               
                   
                   
                 cgctattacgcttggttctgttagtgagactacctacgat 
                   
                 QNAREAYWGSNLPR 
                   
               
               
                   
                   
                 ttcttggacaatgtaaacgaaatcataagaaacaatac 
                   
                 LMQIKSLYDPTDLFH 
                   
               
               
                   
                   
                 accaggacttggtaacggtgtttaccctggttatgttga 
                   
                 NPQGVLPA 
                   
               
               
                   
                   
                 tccaaggttgcaaaatgcaagagaagcctattggggtt 
                   
                   
                   
               
               
                   
                   
                 caaatcttccacgtttgatgcaaattaagtctctatatga 
                   
                   
                   
               
               
                   
                   
                 cccaaccgacttgtttcataacccacaaggtgttttgcc 
                   
                   
                   
               
               
                   
                   
                 tgcc 
                   
                   
                   
               
               
                   
               
               
                 t808046 
                 Library 
                 atggctccatccatttcattttctttgctacaaatctcgctt 
                 80 
                 MAPSISFSLLQISLLA 
                 150 
               
               
                   
                   
                 ttggcctattctggtctggtgagtggagatttctctttaa 
                   
                 YSGLVSGDFSLRQC 
                   
               
               
                   
                   
                 gacagtgcttggaatccgctgttagcagggtagcattc 
                   
                 LESAVSRVAFEGDPF 
                   
               
               
                   
                   
                 gagggcgaccctttttaccaattattgtcagtcagacca 
                   
                 YQLLSVRPYNLDISI 
                   
               
               
                   
                   
                 tacaacttagatatatccattgttccagctgccgtcgctt 
                   
                 VPAAVAFPADTNEV 
                   
               
               
                   
                   
                 tccccgctgacactaatgaagttgcagctgtcgtaaga 
                   
                 AAVVRCAAQNGYQ 
                   
               
               
                   
                   
                 tgtgctgcccaaaacggttatcaagttcaagcaaaaag 
                   
                 VQAKSGGHSYANH 
                   
               
               
                   
                   
                 tggtggtcactcatacgctaatcatggtttgggtggtac 
                   
                 GLGGTNGAVVVNLE 
                   
               
               
                   
                   
                 caacggagctgttgtggttaatctggaaaacttgcaac 
                   
                 NLQHFSMNTTTWEA 
                   
               
               
                   
                   
                 acttctccatgaacacgactacctgggaagccacaat 
                   
                 TIGAGTLLGDVTKR 
                   
               
               
                   
                   
                 cggtgctggtacattattgggtgatgtcaccaagcgttt 
                   
                 LSDAGGRAMAHGT 
                   
               
               
                   
                   
                 gtctgacgctggcggtagagcaatggcccatggtact 
                   
                 CPQVGSGGHFTIGGL 
                   
               
               
                   
                   
                 tgtcctcaggttggttctggaggtcactttactattggtg 
                   
                 GPSSRQFGAALDHII 
                   
               
               
                   
                   
                 gcctaggtccatctagtagacaatttggcgccgctttg 
                   
                 EAEVVLANSSIIRAS 
                   
               
               
                   
                   
                 gatcatatcatagaagctgaagtcgttctagctaactctt 
                   
                 ETENPDVFFAVRGA 
                   
               
               
                   
                   
                 ctattatcagagcatctgagactgaaaacccagatgtg 
                   
                 ASGFGIVTEFKVRTE 
                   
               
               
                   
                   
                 ttcttcgctgtaagaggagctgcttccggttttggtattg 
                   
                 PEPGQAVRYSYSFSF 
                   
               
               
                   
                   
                 ttaccgaatttaaggttcgtaccgaaccagaaccaggt 
                   
                 SDTATRADLFKKWQ 
                   
               
               
                   
                   
                 caagccgtcagatacagttattctttctcgttcagcgac 
                   
                 AYVTQPDLPRELAS 
                   
               
               
                   
                   
                 accgctacgcgtgcagacttgttcaagaaatggcaag 
                   
                 TLTILEHGMFITGTFF 
                   
               
               
                   
                   
                 cctacgtcactcaaccagatttgcctagagaacttgctt 
                   
                 GSKEEYNALKIETEF 
                   
               
               
                   
                   
                 ctactctgacaattttggaacacggtatgttcatcactg 
                   
                 PGFAKGGTLVLDDW 
                   
               
               
                   
                   
                 gtacgtttttcggttcaaaggaggagtacaatgctctaa 
                   
                 LGLVSNWAEDLLLS 
                   
               
               
                   
                   
                 agattgaaaccgaatttcccggtttcgccaagggtgga 
                   
                 EEEIEQMFEYIDNVD 
                   
               
               
                   
                   
                 accttagtcttggatgactggttgggtttagttagtaatt 
                   
                 KGTLLWFAIFDLQG 
                   
               
               
                   
                   
                 gggctgaagacttgcttttgtcggaagaagaaatcga 
                   
                 GAVGDVPVDATAY 
                   
               
               
                   
                   
                 gcaaatgttcgaatatattgataacgttgacaaaggtac 
                   
                 AHRDTLIWLQSYAI 
                   
               
               
                   
                   
                 actactgtggtttgccattttcgacctacaaggtggtgct 
                   
                 NLFGRISETTVEFLE 
                   
               
               
                   
                   
                 gtcggtgatgtaccagtcgatgccactgcttacgctca 
                   
                 RLNELTLTSTAKTVP 
                   
               
               
                   
                   
                 cagagataccttgatatggctacaatcctacgcaatca 
                   
                 YAAYPGYVDPRLTD 
                   
               
               
                   
                   
                 atctgtttggtagaataagcgaaactactgttgagttttt 
                   
                 AQAAYWGSNLARL 
                   
               
               
                   
                   
                 agaacgtttgaacgaattgactttgacatctacagctaa 
                   
                 NRIKAEIDPNNVFHN 
                   
               
               
                   
                   
                 gacggttccatatgcagcctaccctggttatgttgaccc 
                   
                 PQSVRPASG 
                   
               
               
                   
                   
                 aagattgactgatgctcaagctgcctactggggatcga 
                   
                   
                   
               
               
                   
                   
                 acttagctagattgaacagaatcaaagctgaaatcgac 
                   
                   
                   
               
               
                   
                   
                 ccaaacaatgtattccacaatccccaatccgttcgtcca 
                   
                   
                   
               
               
                   
                   
                 gcttctggt 
                   
                   
                   
               
               
                   
               
               
                 t808051 
                 Library 
                 atgggtaatactacctcgatagccgctggcagagattg 
                 81 
                 MGNTTSIAAGRDCL 
                 151 
               
               
                   
                   
                 cctggttaacgctgtcggtggtaaccaggcattagtag 
                   
                 VNAVGGNQALVAF 
                   
               
               
                   
                   
                 cttttcaagaccaattgctatatcaatccacggccgtcg 
                   
                 QDQLLYQSTAVEAY 
                   
               
               
                   
                   
                 aagcttacaacttgaatattcctgttacaccagctgctgt 
                   
                 NLNIPVTPAAVTFPE 
                   
               
               
                   
                   
                 cactttcccagagtcttcagaacaaatcgcagccgtgg 
                   
                 SSEQIAAVVKCASEH 
                   
               
               
                   
                   
                 ttaaatgtgcttctgaacacgactacaaggttcaagctc 
                   
                 DYKVQARSGGHSFG 
                   
               
               
                   
                   
                 gtagcggtggacatagtttcggtaattatggtttgggtg 
                   
                 NYGLGGTNGAIVVD 
                   
               
               
                   
                   
                 gtaccaacggcgccatcgtggttgatatgaagaaattt 
                   
                 MKKFDQFSMDESSY 
                   
               
               
                   
                   
                 gatcaattctccatggacgaatcgtcttacattgctacta 
                   
                 IATIGPGTTLGDVDT 
                   
               
               
                   
                   
                 ttggtcccggtaccactttaggtgatgtcgacacagaa 
                   
                 ELYNAGGRAMAHGI 
                   
               
               
                   
                   
                 ttgtacaacgctggaggtagagccatggctcacggta 
                   
                 CPTIRTGGHLTMGG 
                   
               
               
                   
                   
                 tttgtccaaccatcagaactggcggtcatcttacgatgg 
                   
                 LGPTARQWGLALDH 
                   
               
               
                   
                   
                 gtggtttgggtccaactgccaggcagtggggcttggc 
                   
                 IEEVEVVLANSSIVR 
                   
               
               
                   
                   
                 tctggaccacatagaagaggttgaagtcgtattagcta 
                   
                 ASHTQNQDILFAVK 
                   
               
               
                   
                   
                 attcttccatcgttagagcatctcatacccaaaaccaag 
                   
                 GASASFGIVTEFKVR 
                   
               
               
                   
                   
                 atattttgtttgccgttaagggtgcttccgcatcattcggt 
                   
                 TEPAPGLAVQYSYT 
                   
               
               
                   
                   
                 attgtcactgaatttaaggttagaactgaacctgcacca 
                   
                 FNLGSAASKAKLVK 
                   
               
               
                   
                   
                 ggtttggctgtccaatactcttataccttcaatttgggta 
                   
                 DWQEFIAQDNLTWK 
                   
               
               
                   
                   
                 gtgcagcctccaaggctaaattagttaaggattggcaa 
                   
                 FYSNMVIIDGDIILEG 
                   
               
               
                   
                   
                 gagttcatcgctcaggacaacttgacatggaaattctat 
                   
                 IFFGSKEEFDALELE 
                   
               
               
                   
                   
                 agcaatatggtcattatcgacggagatataattctggaa 
                   
                 NRFPPKNPGNILVLT 
                   
               
               
                   
                   
                 ggtatctttttcggttctaaggaagaatttgatgctttaga 
                   
                 DWLGMISHSLEDIIL 
                   
               
               
                   
                   
                 actagaaaacaggttcccacccaagaacccaggtaa 
                   
                 RVAGGVPTYFYAKS 
                   
               
               
                   
                   
                 catacttgtgttgactgattggttgggaatgatttctcact 
                   
                 LGFTPQALIPSSAIDD 
                   
               
               
                   
                   
                 ccttggaagacatcattttaagagttgctggtggtgtac 
                   
                 LFDYIEKTNPGTLA 
                   
               
               
                   
                   
                 caacctacttttacgctaagtccttaggtttcacacctca 
                   
                 WFITLSLEGGAINNV 
                   
               
               
                   
                   
                 agctttgatcccatctagcgctattgatgacctgttcgat 
                   
                 PADATAYGHRDVLH 
                   
               
               
                   
                   
                 tatatagaaaagactaatccaggtactctagcctggttt 
                   
                 WVQIFAANPLGPISE 
                   
               
               
                   
                   
                 atcaccttgtccttggagggcggagctattaacaacgt 
                   
                 TTYDFTDGLYNILA 
                   
               
               
                   
                   
                 tccagctgacgcaacagcctacggtcacagagatgtg 
                   
                 KAVPESAEHAYLGC 
                   
               
               
                   
                   
                 cttcattgggtccaaatctttgccgctaatcctttgggtc 
                   
                 PDPRMKDAQKAYW 
                   
               
               
                   
                   
                 caatttctgaaaccacttacgacttcactgacggtttata 
                   
                 RDNLPRLEELKAEL 
                   
               
               
                   
                   
                 caacatccttgctaaagccgttcctgagtctgctgaac 
                   
                 DPKDTFHNPQGVAV 
                   
               
               
                   
                   
                 atgcttatttaggttgtcctgatccacgtatgaaagacg 
                   
                 A 
                   
               
               
                   
                   
                 ctcaaaaggcttactggagagataacctgccacgtttg 
                   
                   
                   
               
               
                   
                   
                 gaagaattaaaggctgaattggatcccaaagatactttt 
                   
                   
                   
               
               
                   
                   
                 cacaatccacaaggtgtagccgtcgct 
                   
                   
                   
               
               
                   
               
               
                 t808061 
                 Library 
                 atgttattgaaactatttttcttggccgtagcagcttcagt 
                 82 
                 MLLKLFFLAVAASV 
                 152 
               
               
                   
                   
                 tgctctggctgcttccagtgaggccttgaagcagtgctt 
                   
                 ALAASSEALKQCLE 
                   
               
               
                   
                   
                 ggaaaacgtcttcactgaccgtgcaggctttgctttcg 
                   
                 NVFTDRAGFAFAGD 
                   
               
               
                   
                   
                 ccggtgatttattctatgacagaatagttaatagatacaa 
                   
                 LFYDRIVNRYNLNIP 
                   
               
               
                   
                   
                 cttgaatatcccagtcaccccttcggctttggcttttcca 
                   
                 VTPSALAFPTSSQQV 
                   
               
               
                   
                   
                 acgagctctcaacaagttgccgatattgtgaagtgtgc 
                   
                 ADIVKCAADNGYPV 
                   
               
               
                   
                   
                 agctgataacggttaccccgttcaagctaggtccgga 
                   
                 QARSGGHSYGNYGL 
                   
               
               
                   
                   
                 ggtcattcttatggtaactacggtcttggtggtgctgac 
                   
                 GGADGAVAIDLKHL 
                   
               
               
                   
                   
                 ggcgccgtcgctatcgatttaaaacacctacaacaatt 
                   
                 QQFSMDKTTWQATI 
                   
               
               
                   
                   
                 ctctatggacaagacaacttggcaggctaccattggtg 
                   
                 GAGSLLSDVTQRLS 
                   
               
               
                   
                   
                 ccggatctttgctatccgatgttacccaaagattgagcc 
                   
                 HAGGRAMSHGICPQ 
                   
               
               
                   
                   
                 acgctggtggcagagccatgtctcatggtatttgtcca 
                   
                 VGSGGHFTIGGLGPT 
                   
               
               
                   
                   
                 caagtcggttcgggtggtcacttcacaatcggtggttt 
                   
                 SRQFGAALDHVLEV 
                   
               
               
                   
                   
                 gggaccaacttcaagacaatttggtgctgccttagacc 
                   
                 EVVLANSSIVRASDT 
                   
               
               
                   
                   
                 atgttcttgaagtcgaagtcgttttggctaattccagtatt 
                   
                 ENKDLFWAIKGAAS 
                   
               
               
                   
                   
                 gtccgtgcttctgatactgaaaacaaggatttgttttgg 
                   
                 GYGIVTEFKVRTEPE 
                   
               
               
                   
                   
                 gctattaagggtgctgcatctggatacggtatcgttacc 
                   
                 PGTAVQYAYSMEFG 
                   
               
               
                   
                   
                 gaatttaaagtgagaactgaacctgaaccaggtaccg 
                   
                 NPTKQATLFKSWQA 
                   
               
               
                   
                   
                 ctgttcaatatgcatacagcatggagttcggtaatccaa 
                   
                 FVSDPKLTRKMAST 
                   
               
               
                   
                   
                 ctaagcaagcaacccttttcaagtcctggcaggcttttg 
                   
                 LTMLENSMAISGTFF 
                   
               
               
                   
                   
                 tgtctgacccaaaattgactagaaagatggcctctaca 
                   
                 GTKEEYDKLNLTNK 
                   
               
               
                   
                   
                 ttaacgatgctggaaaacagtatggctatatccggtact 
                   
                 FPGANGDALVFEDW 
                   
               
               
                   
                   
                 ttcttcggtactaaggaagaatacgacaagttgaatttg 
                   
                 LGLVAHWAEDLILG 
                   
               
               
                   
                   
                 accaacaagtttcctggtgctaatggtgacgctttagttt 
                   
                 LAAGIPTNFYAKSTS 
                   
               
               
                   
                   
                 tcgaagattggctgggcctagtggctcactgggctga 
                   
                 WTPQTLITPETVDK 
                   
               
               
                   
                   
                 ggatttgatattgggtttagctgccggtattccaactaac 
                   
                 MFDYIATVNKGTLG 
                   
               
               
                   
                   
                 ttctatgccaaatcaacgtcttggactccccaaacatta 
                   
                 WFLLFDLQGGYTND 
                   
               
               
                   
                   
                 atcacccccgaaaccgtagataaaatgtttgactacat 
                   
                 IPTNATSYAHRDVLI 
                   
               
               
                   
                   
                 cgccaccgttaacaaaggtactcttggctggttcttatt 
                   
                 WLQSYTVNFLGPISQ 
                   
               
               
                   
                   
                 gtttgacttgcaaggtggttatacgaacgatattccaac 
                   
                 AQIDFLDGLNKIVTN 
                   
               
               
                   
                   
                 caacgccacatcatacgctcacagagatgtcttgattt 
                   
                 NKLPYTAYPGYVDP 
                   
               
               
                   
                   
                 ggctacaatcttatacagttaactttttgggtcctatctcc 
                   
                 LMPNAPEAYWGTN 
                   
               
               
                   
                   
                 caggctcaaattgacttcctagatggtttgaataagatt 
                   
                 LPRLQQIKELVDPND 
                   
               
               
                   
                   
                 gtcaccaacaataagttgccatacactgcttacccagg 
                   
                 VFRNPQSPSPANKEP 
                   
               
               
                   
                   
                 ttacgttgatccattgatgccaaatgctccagaagcata 
                   
                 L 
                   
               
               
                   
                   
                 ctggggaactaacttgccaagattacaacaaatcaag 
                   
                   
                   
               
               
                   
                   
                 gaattagtcgaccctaatgatgtttttcgtaacccacaat 
                   
                   
                   
               
               
                   
                   
                 ctccatccccagctaacaaagagccactg 
                   
                   
                   
               
               
                   
               
               
                 t808069 
                 Library 
                 atgggtaacggaaatagcacaccttttcgtgactgttta 
                 83 
                 MGNGNSTPFRDCLD 
                 153 
               
               
                   
                   
                 gattctatatgcgcaaacagatccacctgtgtgacgtat 
                   
                 SICANRSTCVTYPGD 
                   
               
               
                   
                   
                 ccaggtgacccactgttctcgtgttggagtaggccctt 
                   
                 PLFSCWSRPFNLEFP 
                   
               
               
                   
                   
                 caatttggagtttcctgtagtcccagccgctatcattag 
                   
                 VVPAAIIRPETTTEV 
                   
               
               
                   
                   
                 accagaaactaccactgaagttgctgaaactgttaaat 
                   
                 AETVKCAKKYGYK 
                   
               
               
                   
                   
                 gtgctaagaagtacggttacaaggttcaggctaaatca 
                   
                 VQAKSGGHSYGNH 
                   
               
               
                   
                   
                 ggtggccactcctacggtaaccatggtttgggtggtgt 
                   
                 GLGGVGGAVSIDMV 
                   
               
               
                   
                   
                 cggaggtgccgtcagtattgatatggtcaacctaaga 
                   
                 NLRDFSMNNKTWY 
                   
               
               
                   
                   
                 gatttctctatgaacaataagacctggtatgcttctttcg 
                   
                 ASFGSGMNLGELDE 
                   
               
               
                   
                   
                 gttctggtatgaaccttggtgaattggacgagcacttac 
                   
                 HLHANGRRAIAHGT 
                   
               
               
                   
                   
                 atgccaacggcagaagagcaatcgctcacggtacat 
                   
                 CPGVGTGGHLTVGG 
                   
               
               
                   
                   
                 gcccaggtgttggtactggtggtcatttgaccgttggtg 
                   
                 LGPISRQWGSALDH 
                   
               
               
                   
                   
                 gtttgggtccaatttccagacaatggggctctgctctgg 
                   
                 LLEIEVITADGTVQR 
                   
               
               
                   
                   
                 accacttgctagaaatcgaagtcatcactgctgatggt 
                   
                 ASYTKNSGLFWALR 
                   
               
               
                   
                   
                 acggtgcaaagagcctcatatactaaaaattctggatt 
                   
                 GAGASFGIVTKFMV 
                   
               
               
                   
                   
                 attttgggctttgcgtggtgctggcgcctctttcggtatt 
                   
                 KTHPEPGRVVQYSY 
                   
               
               
                   
                   
                 gttacaaagtttatggttaagactcacccagaacctggt 
                   
                 NIALASHAETAELYR 
                   
               
               
                   
                   
                 agagtagtgcaatactcatacaatatagctttggcctcc 
                   
                 EWQALVGDPNMDR 
                   
               
               
                   
                   
                 catgctgaaactgctgaactatatagggaatggcaag 
                   
                 RFSSLFVVQPLGALI 
                   
               
               
                   
                   
                 ccttggttggagatccaaacatggaccgtagattctctt 
                   
                 TGTFFGTKSQYQAT 
                   
               
               
                   
                   
                 ccttattcgtcgtccaaccattgggtgctttgattaccgg 
                   
                 GIPDRLPGADKGAV 
                   
               
               
                   
                   
                 taccttctttggtaccaagtcccaataccaggcaactg 
                   
                 WLTDWAGLLLHEA 
                   
               
               
                   
                   
                 gtattcctgacagactaccaggtgctgataaaggtgct 
                   
                 EAAGCALGSIPTAFY 
                   
               
               
                   
                   
                 gtctggcttacagattgggcaggcttgttattgcacgaa 
                   
                 GKSLSLSEQDLLSDS 
                   
               
               
                   
                   
                 gctgaggccgctggttgtgccttaggtagcatcccaa 
                   
                 AITDLFKYLEDNRSG 
                   
               
               
                   
                   
                 ccgctttctacggcaagtcgttgtctttgagtgaacaag 
                   
                 LAPVTILFNTEGGA 
                   
               
               
                   
                   
                 accttttatcagattctgctattaccgacttgtttaagtatt 
                   
                 MMDTPANATAYPH 
                   
               
               
                   
                   
                 tagaggataacagatccggtttagcccccgttactatct 
                   
                 RNSIIMYQSYGIGVG 
                   
               
               
                   
                   
                 tgtttaataccgaaggtggtgctatgatggatacgcctg 
                   
                 KVSAATRKLLDGVH 
                   
               
               
                   
                   
                 ccaacgccactgcttacccccacagaaactccattatc 
                   
                 ERIQRSAPGALSTYA 
                   
               
               
                   
                   
                 atgtaccaatcttatggtataggagttggtaaggttagt 
                   
                 GYIDAWADRKAAQ 
                   
               
               
                   
                   
                 gctgcaacacgtaaactgttggacggtgttcatgaaag 
                   
                 KLYWADNLPRLREL 
                   
               
               
                   
                   
                 aatccaaagaagcgcaccaggcgctctgtctacttac 
                   
                 KKVWDPADVFSNPQ 
                   
               
               
                   
                   
                 gctggttatattgacgcctgggctgaccgtaaggctgc 
                   
                 SVEPAD 
                   
               
               
                   
                   
                 ccaaaagctatactgggctgataatttgccaagattaa 
                   
                   
                   
               
               
                   
                   
                 gagaattaaaaaaggtctgggatccagcagatgttttc 
                   
                   
                   
               
               
                   
                   
                 tcaaacccacagtctgttgagccagcagac 
                   
                   
                   
               
               
                   
               
               
                 t808076 
                 Library 
                 atggattctaacacttgggaggccacgttcggctcag 
                 84 
                 MDSNTWEATFGSGF 
                 154 
               
               
                   
                   
                 gatttttacttggtgaactagacaaacatttgcacgctaa 
                   
                 LLGELDKHLHANGN 
                   
               
               
                   
                   
                 tggtaacagggctatggcacacggtacctgtccaggt 
                   
                 RAMAHGTCPGVGM 
                   
               
               
                   
                   
                 gttggtatgggtggtcatgccactatcggaggtattgg 
                   
                 GGHATIGGIGPSSRL 
                   
               
               
                   
                   
                 ccctagctccagactgtggggtacaaccttagaccac 
                   
                 WGTTLDHVLQVEV 
                   
               
               
                   
                   
                 gtattgcaggtcgaagtggttactgctgatggtaagat 
                   
                 VTADGKIQRASKTQ 
                   
               
               
                   
                   
                 acaacgtgcttctaagactcaaaacccagatttgttctg 
                   
                 NPDLFWALQGAGAS 
                   
               
               
                   
                   
                 ggctctacaaggtgctggtgcctcgtttggcattatcac 
                   
                 FGIITEFVVRTEPEPG 
                   
               
               
                   
                   
                 cgaatttgtcgttagaaccgaacccgaaccaggtagt 
                   
                 SVVEYTYSVSLGKQ 
                   
               
               
                   
                   
                 gttgtcgaatacacctattccgtatctttgggaaagcaa 
                   
                 SDMAPLYKQWQAL 
                   
               
               
                   
                   
                 tctgacatggctccattgtacaaacaatggcaagctttg 
                   
                 VGDPSLDRRFTSLFI 
                   
               
               
                   
                   
                 gttggtgatccttccctggacagaagattcacaagttta 
                   
                 AEPLGVLITGTFYGT 
                   
               
               
                   
                   
                 ttcattgccgagccattgggtgttttaatcactggtacat 
                   
                 MYEWHASGIPDKLP 
                   
               
               
                   
                   
                 tttatggtactatgtacgaatggcacgcatcaggtatcc 
                   
                 RGPISVTVMDSLGSL 
                   
               
               
                   
                   
                 ctgataagttgccaagaggtccaatttcggtcaccgtta 
                   
                 AHIAEKTGLYLTNV 
                   
               
               
                   
                   
                 tggactctttgggatctttagctcatattgccgaaaaaa 
                   
                 PTSFASRSLALRQQD 
                   
               
               
                   
                   
                 ctggcctgtacttgaccaatgtcccaacgtccttcgcta 
                   
                 LLSEQSIDDLFEYMG 
                   
               
               
                   
                   
                 gcagatctcttgccttgagacagcaagatttgttgtccg 
                   
                 SANADTPLWFVIFD 
                   
               
               
                   
                   
                 agcaatctatcgatgacttattcgaatatatgggttcgg 
                   
                 NEGGAIADVPDNST 
                   
               
               
                   
                   
                 ctaacgcagacactccactttggttcgtgatctttgaca 
                   
                 AYPHRDKVIVYQSY 
                   
               
               
                   
                   
                 acgaaggtggtgctattgctgatgtgcctgataatagc 
                   
                 SVGLLGVTDKMIKF 
                   
               
               
                   
                   
                 accgcctacccacatagagataaggttattgtttaccaa 
                   
                 LDGVQDIVQRGAPN 
                   
               
               
                   
                   
                 agctactccgtcggtttactaggtgtcactgataaaatg 
                   
                 AHTTYAGYINPQLD 
                   
               
               
                   
                   
                 ataaagttcttggacggtgttcaagatattgtccagagg 
                   
                 RKAAQQFYWGDKL 
                   
               
               
                   
                   
                 ggagctcccaacgcccacacgacctatgcaggttac 
                   
                 PRLQQIKKQYDPNN 
                   
               
               
                   
                   
                 atcaatccacaattggaccgtaaggctgctcaacaatt 
                   
                 VFCNPQSIYPAEDMS 
                   
               
               
                   
                   
                 ctattggggtgacaagctaccaagattgcaacagatta 
                   
                 DG 
                   
               
               
                   
                   
                 agaagcaatatgatcctaacaacgtgttttgcaatccac 
                   
                   
                   
               
               
                   
                   
                 aatctatctacccagctgaagacatgtctgacggt 
                   
                   
                   
               
               
                   
               
               
                 t808093 
                 Library 
                 atgggtaacacaacttccatcgcaggcagagattgctt 
                 85 
                 MGNTTSIAGRDCLV 
                 155 
               
               
                   
                   
                 agtctcagcccttggaggtaattctgctttggctgctttc 
                   
                 SALGGNSALAAFPN 
                   
               
               
                   
                   
                 ccaaaccaattgctgtggaccgccgacgttcacgagt 
                   
                 QLLWTADVHEYNL 
                   
               
               
                   
                   
                 ataatttgaacctacctgtaacgccagctgccataacct 
                   
                 NLPVTPAAITYPETA 
                   
               
               
                   
                   
                 accccgaaactgctgaacagattgctggtatcgttaag 
                   
                 EQIAGIVKCASDYD 
                   
               
               
                   
                   
                 tgtgctagtgattacgactataaagtgcaagctaggtct 
                   
                 YKVQARSGGHSFGN 
                   
               
               
                   
                   
                 ggtggtcattcctttggtaattacggtttgggaggtact 
                   
                 YGLGGTDGAVVVD 
                   
               
               
                   
                   
                 gatggtgccgttgtcgtcgacatgaagcacttcaacca 
                   
                 MKHFNQFSMNDQT 
                   
               
               
                   
                   
                 attctcgatgaacgatcaaacctacgaagcagttattg 
                   
                 YEAVIGPGTTLNDV 
                   
               
               
                   
                   
                 gtccaggtactaccttaaacgacgttgacattgaattgt 
                   
                 DIELYNNGKRAMAH 
                   
               
               
                   
                   
                 acaacaatggcaagagagctatggctcatggtgtttgt 
                   
                 GVCPTIKTGGHFTIG 
                   
               
               
                   
                   
                 ccaactatcaaaacaggtggtcactttacaattggcgg 
                   
                 GLGPTARQWGLALD 
                   
               
               
                   
                   
                 tctgggtcctactgccagacaatggggtttggctttaga 
                   
                 HVEEVEVVLANSSIV 
                   
               
               
                   
                   
                 tcacgtcgaagaagtggaagtagtcttggccaactctt 
                   
                 RASNTQNQDVFFAV 
                   
               
               
                   
                   
                 ctatcgttcgtgctagcaatacccaaaaccaggatgtc 
                   
                 KGAAADFGIVTEFK 
                   
               
               
                   
                   
                 ttctttgctgtcaagggcgcagctgccgacttcggtatc 
                   
                 VRTEPAPGLAVQYS 
                   
               
               
                   
                   
                 gttacggagttcaaggttagaactgagccagcacctg 
                   
                 YTFNLGSTAEKAQF 
                   
               
               
                   
                   
                 gtttagctgttcaatattcgtatacctttaatcttggtagta 
                   
                 VKDWQSFISAKNLT 
                   
               
               
                   
                   
                 ctgctgaaaaagcccaatttgtcaaggattggcaaag 
                   
                 RQFYNNMVIFDGDII 
                   
               
               
                   
                   
                 cttcatttccgctaaaaacttgactcgtcaattctacaac 
                   
                 LEGLFFGSKEQYDA 
                   
               
               
                   
                   
                 aatatggttatatttgacggtgacattattttagaaggttt 
                   
                 LGLEDHFAPKNPGNI 
                   
               
               
                   
                   
                 gtttttcggatcaaaggaacaatacgatgccttgggttt 
                   
                 LVLTDWLGMVGHA 
                   
               
               
                   
                   
                 ggaagatcattttgctccaaagaatccaggtaacatcc 
                   
                 LEDTILKLVGNTPTW 
                   
               
               
                   
                   
                 tagtgctgacggactggttgggaatggtaggtcatgct 
                   
                 FYAKSLGFRQDTLIP 
                   
               
               
                   
                   
                 ttggaagacaccattttgaagctagttggaaacacacc 
                   
                 SAGIDEFFEYIANHT 
                   
               
               
                   
                   
                 cacttggttctacgctaaatctttgggtttcagacaagat 
                   
                 AGTPAWFVTLSLEG 
                   
               
               
                   
                   
                 accctaatcccatctgctggtattgacgaatttttcgaat 
                   
                 GAINDVAEDATAYA 
                   
               
               
                   
                   
                 atatagcaaaccacaccgctggtactccagcttggttc 
                   
                 HRDVLFWVQLFMV 
                   
               
               
                   
                   
                 gttaccttatctctggaaggcggcgctataaacgatgt 
                   
                 NPLGPISETTYEFTD 
                   
               
               
                   
                   
                 ggctgaagatgccacagcatacgcacacagagatgt 
                   
                 GLYDVLARAVPESV 
                   
               
               
                   
                   
                 cctattttgggttcagttgttcatggtcaatccactaggt 
                   
                 GHAYLGCPDPRMEN 
                   
               
               
                   
                   
                 ccaatctcagaaactacctacgagttcactgacggttta 
                   
                 APQKYWRTNLPRLQ 
                   
               
               
                   
                   
                 tatgacgtcttagcaagagctgtccctgaatctgttggt 
                   
                 ELKEELDPKNTFHHP 
                   
               
               
                   
                   
                 catgcctatttgggttgtccagacccaagaatggaaaa 
                   
                 QGVIPA 
                   
               
               
                   
                   
                 cgctccacaaaagtactggcgtactaatttgcctagatt 
                   
                   
                   
               
               
                   
                   
                 acaagaattgaaagaggaattggatccaaagaacac 
                   
                   
                   
               
               
                   
                   
                 cttccaccatccacaaggtgtgattccagct 
                   
                   
                   
               
               
                   
               
               
                 t808094 
                 Library 
                 atgggtaacactacgtcgattgccgcaggcagagatt 
                 86 
                 MGNTTSIAAGRDCL 
                 156 
               
               
                   
                   
                 gccttgtcagtgctgttggtggtgtggctgctcatgttg 
                   
                 VSAVGGVAAHVAF 
                   
               
               
                   
                   
                 cttttcaggactctttgttataccaagccacagccgtag 
                   
                 QDSLLYQATAVELY 
                   
               
               
                   
                   
                 agctgtataatctaaacatacctgtcacccccgctgctg 
                   
                 NLNIPVTPAAVTYPQ 
                   
               
               
                   
                   
                 ttacttacccacaaagcaccgatgaaatcgccgctgtc 
                   
                 STDEIAAVVKCASD 
                   
               
               
                   
                   
                 gttaaatgtgcttcagactatgactacaaggttcaagct 
                   
                 YDYKVQARSGGHSF 
                   
               
               
                   
                   
                 cgttccggtggtcactccttcggaaactacggtttgggt 
                   
                 GNYGLGGQNGAIVI 
                   
               
               
                   
                   
                 ggccaaaatggtgcaattgtaatcgatatgaagcactt 
                   
                 DMKHFSQFSLDKST 
                   
               
               
                   
                   
                 ctctcaattttctttagataagtctactttcattgccacctt 
                   
                 FIATFGPGTTLGNLD 
                   
               
               
                   
                   
                 cggtccaggtactacattgggaaacttggacaccgaa 
                   
                 TELYHAGNRAMAH 
                   
               
               
                   
                   
                 ctatatcatgctggtaacagagcaatggctcacggtat 
                   
                 GICPTIRTGGHLTMG 
                   
               
               
                   
                   
                 ctgtccaactattagaaccggaggtcatttgacaatgg 
                   
                 GLGPAARQWGLAL 
                   
               
               
                   
                   
                 gcggtttgggtccagctgccaggcagtggggtttggc 
                   
                 DHVEEVEVVLANSS 
                   
               
               
                   
                   
                 attagatcacgttgaagaagtcgaagttgtccttgctaa 
                   
                 VVRASDTQNQDVFF 
                   
               
               
                   
                   
                 ttccagcgtggtaagagcctctgacactcaaaatcaag 
                   
                 AVKGAAASFGIVTE 
                   
               
               
                   
                   
                 acgttttctttgctgttaaaggtgctgctgcttcttttggta 
                   
                 FKVRTEEAPGLAVQ 
                   
               
               
                   
                   
                 tcgtcactgagttcaaggttcgtactgaagaagcccct 
                   
                 YSFPFNLGTPAEKAK 
                   
               
               
                   
                   
                 ggtttggctgttcaatacagctttccattcaacttgggta 
                   
                 LVKDWQAFIAQENL 
                   
               
               
                   
                   
                 ccccagctgaaaaagctaagttagttaaggattggca 
                   
                 SWKFYSNMVIFDGQ 
                   
               
               
                   
                   
                 agcatttatagctcaagaaaatttatcgtggaagttctac 
                   
                 IILEGIFFGSKKEYDE 
                   
               
               
                   
                   
                 tcaaacatggtcatctttgatggtcaaattattctggagg 
                   
                 LDLENKFPTSEPGTV 
                   
               
               
                   
                   
                 gcattttcttcggctccaaaaaggaatatgacgaattgg 
                   
                 LVLTDWLGMIGHGL 
                   
               
               
                   
                   
                 acctggaaaacaagttccccacctcggaaccaggtac 
                   
                 EDTILRLVGNSPTWF 
                   
               
               
                   
                   
                 agtcttggtcttgaccgattggcttggtatgatcggtca 
                   
                 YAKSLGFTPSTLISD 
                   
               
               
                   
                   
                 cggtttggaagacactattttaagattggtgggtaactc 
                   
                 SAIDGLFDYIHKTNP 
                   
               
               
                   
                   
                 cccaacatggttctacgccaagtctcttggctttactcct 
                   
                 GTLAWFVTLSLEGG 
                   
               
               
                   
                   
                 tctactttaattagtgatagtgctatcgatggtttgttcgat 
                   
                 AINTVSEDATAYGH 
                   
               
               
                   
                   
                 tatatccacaaaaccaacccaggtacattggcctggttt 
                   
                 RDVLFWVQIFVANP 
                   
               
               
                   
                   
                 gttacgctatctttggagggtggagctataaatactgtc 
                   
                 LGPISQTTYDFADGL 
                   
               
               
                   
                   
                 tccgaagatgccactgcttacggacatagagatgtttt 
                   
                 YNVLAQAVPDSAGH 
                   
               
               
                   
                   
                 gttctgggttcaaatctttgttgctaaccctttgggtcca 
                   
                 AYLGCPDPKLPDAQ 
                   
               
               
                   
                   
                 atttcacagactacctacgacttcgctgacggattatac 
                   
                 RAYWRSNLPRLEEL 
                   
               
               
                   
                   
                 aacgttctggctcaagctgtgccagattctgccggtca 
                   
                 KRDLDPKDIFYNPQ 
                   
               
               
                   
                   
                 tgcttacctaggttgtccagaccctaaattgccagatgc 
                   
                 GVQIVS 
                   
               
               
                   
                   
                 tcagagagcatactggaggtctaatctaccaagactg 
                   
                   
                   
               
               
                   
                   
                 gaggaacttaagagagacttggatccaaaagacatct 
                   
                   
                   
               
               
                   
                   
                 tctataatccacaaggtgtccaaattgtttcc 
                   
                   
                   
               
               
                   
               
               
                 t808103 
                 Library 
                 atgggtaacaccacatcgatcgctgccggacgtgact 
                 87 
                 MGNTTSIAAGRDCL 
                 157 
               
               
                   
                   
                 gcttactttccgcagtcggtggcaatcatgctcacgtg 
                   
                 LSAVGGNHAHVAFQ 
                   
               
               
                   
                   
                 gctttccaagatcagctgctataccaagccactgctgtt 
                   
                 DQLLYQATAVEPYN 
                   
               
               
                   
                   
                 gaaccttataacttgaatataccagtaacgcccgctgc 
                   
                 LNIPVTPAAVTYPQS 
                   
               
               
                   
                   
                 cgttacttacccacaatcagctgacgaggttgctgccg 
                   
                 ADEVAAVVKCAAD 
                   
               
               
                   
                   
                 tcgttaaatgtgcagctgattacggttataaggtccaag 
                   
                 YGYKVQARSGGHSF 
                   
               
               
                   
                   
                 ctagaagtggtggtcactcttttggtaactacggtttgg 
                   
                 GNYGLGGEDGAIVV 
                   
               
               
                   
                   
                 gtggcgaagatggtgctattgttgtggacatgaagcat 
                   
                 DMKHFDQFSMDEST 
                   
               
               
                   
                   
                 ttcgatcaattttctatggacgaatctacctatacagcca 
                   
                 YTATIGPGITLGDLD 
                   
               
               
                   
                   
                 ctattggtccaggtatcactttgggcgatttggacaccg 
                   
                 TALYNAGHRAMAH 
                   
               
               
                   
                   
                 ctttatacaatgcaggtcacagagccatggctcacggt 
                   
                 GICPTIRTGGHLTIGG 
                   
               
               
                   
                   
                 atttgtccaaccatcaggacgggtggtcacttgactata 
                   
                 LGPTARQWGLALDH 
                   
               
               
                   
                   
                 ggaggtttaggtcctactgctagacagtggggacttgc 
                   
                 VEEVEVVLANSSIVR 
                   
               
               
                   
                   
                 cttggatcatgtagaagaggttgaagtcgttctggctaa 
                   
                 ASDTQNQEILFAVK 
                   
               
               
                   
                   
                 cagctccattgtcagagcctctgacacacaaaaccaa 
                   
                 GAAASFGIVTEFKVR 
                   
               
               
                   
                   
                 gaaatcttgttcgccgttaagggtgctgctgcttccttc 
                   
                 TEEAPGLAVQYSFTF 
                   
               
               
                   
                   
                 ggaatcgtcaccgaatttaaagttcgtactgaagaagc 
                   
                 NLGTAAEKAKLVKD 
                   
               
               
                   
                   
                 tccaggtttggctgtccaatactccttcacctttaatttgg 
                   
                 WQAFIAQEDLTWKF 
                   
               
               
                   
                   
                 gtactgctgccgagaaggcaaagttagttaaagattg 
                   
                 YSNMNIIDGQIILEGI 
                   
               
               
                   
                   
                 gcaagccttcattgctcaagaagatcttacttggaagtt 
                   
                 YFGSKAEYDALGLE 
                   
               
               
                   
                   
                 ctattctaacatgaacataattgacggtcaaatcattctg 
                   
                 EKFPTSEPGTVLVLT 
                   
               
               
                   
                   
                 gaaggtatctatttcggttcaaaagccgaatacgacgc 
                   
                 DWLGMVGHGLEDV 
                   
               
               
                   
                   
                 tttaggtttggaagaaaagtttccaacttctgagccagg 
                   
                 ILRLVGNAPTWFYA 
                   
               
               
                   
                   
                 caccgtgttggtactaactgactggttgggtatggttgg 
                   
                 KSLGFAPRALIPDSAI 
                   
               
               
                   
                   
                 tcatggtttggaagatgtaattttaagattggtcggtaat 
                   
                 DDFFEYIHKNNPGT 
                   
               
               
                   
                   
                 gctcccacctggttctacgctaagtcgctaggttttgca 
                   
                 VSWFVTLSLEGGAI 
                   
               
               
                   
                   
                 ccaagagctctaattcctgattccgcaatagacgattttt 
                   
                 NKVPEDATAYGHRD 
                   
               
               
                   
                   
                 tcgaatacattcacaaaaacaatccaggtaccgtttcat 
                   
                 VLFWVQIFMINPLGP 
                   
               
               
                   
                   
                 ggtttgttaccttgtctttggaaggcggtgccatcaaca 
                   
                 VSQTIYDFADGLYD 
                   
               
               
                   
                   
                 aggtccccgaagatgctactgcttatggccatagagat 
                   
                 VLAKAVPESAGHAY 
                   
               
               
                   
                   
                 gttctattctgggtccagattttcatgatcaacccattgg 
                   
                 LGCPDPRMPNAQQA 
                   
               
               
                   
                   
                 gtccagtttctcaaacaatttacgatttcgctgacggtct 
                   
                 YWRNNLPRLEELKG 
                   
               
               
                   
                   
                 gtatgacgtcttagctaaggcagtgccagaaagcgcc 
                   
                 DLDPKDIFHNPQGV 
                   
               
               
                   
                   
                 ggtcacgcatacttgggttgtccagatcctcgtatgcct 
                   
                 MVVS 
                   
               
               
                   
                   
                 aacgctcaacaagcctactggagaaacaacttgccaa 
                   
                   
                   
               
               
                   
                   
                 gactggaagagttaaagggtgatcttgacccaaaaga 
                   
                   
                   
               
               
                   
                   
                 cattttccataatccacaaggtgtcatggttgtctcc 
                   
                   
                   
               
               
                   
               
               
                 t808125 
                 Library 
                 atgggtaacggacagtccacccccttgcaacaatgttt 
                 88 
                 MGNGQSTPLQQCLN 
                 158 
               
               
                   
                   
                 aaatactgtttgcaacggtagactaggttgtgtagctttc 
                   
                 TVCNGRLGCVAFPS 
                   
               
               
                   
                   
                 ccaagtgatgcattgtatcaagccgcttgggtcaagcc 
                   
                 DALYQAAWVKPYN 
                   
               
               
                   
                   
                 ttacaacctggacgtgccagttacgcctatcgctgttttt 
                   
                 LDVPVTPIAVFKPSS 
                   
               
               
                   
                   
                 aaaccaagctctacagaggatgtcgccggtgctataa 
                   
                 TEDVAGAIKCAVAS 
                   
               
               
                   
                   
                 agtgtgctgttgcctcgaatgtgcacgttcaagcaaag 
                   
                 NVHVQAKSGGHSY 
                   
               
               
                   
                   
                 tccggtggccattcttacgctaacttcggtttgggtggt 
                   
                 ANFGLGGQDGELMI 
                   
               
               
                   
                   
                 caagacggagaattaatgattgacttggctaaccttca 
                   
                 DLANLQDFHMDKTS 
                   
               
               
                   
                   
                 ggattttcacatggacaaaacttcttggcaagctactttc 
                   
                 WQATFGAGYRLGD 
                   
               
               
                   
                   
                 ggtgctggttataggttaggcgatttggataagaagttg 
                   
                 LDKKLQANGNRAIA 
                   
               
               
                   
                   
                 caagccaatggtaatagagccattgctcatggtacctg 
                   
                 HGTCPGVGIGGHATI 
                   
               
               
                   
                   
                 tccaggagtcggtatcggtggtcacgccactattggtg 
                   
                 GGLGPMSRMWGSA 
                   
               
               
                   
                   
                 gtctaggtccaatgtcacgtatgtggggcagtgctttg 
                   
                 LDHVLSVQVVTADG 
                   
               
               
                   
                   
                 gaccatgtcttatctgttcaagtagtgaccgctgatggtt 
                   
                 SIKNASESENSDLFW 
                   
               
               
                   
                   
                 ccatcaaaaacgcatccgaatctgaaaactcagatctg 
                   
                 ALRGAGASFGVITKF 
                   
               
               
                   
                   
                 ttttgggctttgagaggagctggtgccagcttcggtgtc 
                   
                 TVKTHPAPGSVVQY 
                   
               
               
                   
                   
                 ataaccaagttcacagttaaaactcaccctgctcccgg 
                   
                 TYKISLGSQAQMAP 
                   
               
               
                   
                   
                 ttctgtcgtacaatacacttacaagatttcgttgggttctc 
                   
                 VYAAWQALAGDPK 
                   
               
               
                   
                   
                 aggcccaaatggctccagtttatgcagcttggcaagct 
                   
                 LDRRFSTLFIAEPLG 
                   
               
               
                   
                   
                 ttagctggtgacccaaagcttgacagacgtttctctaca 
                   
                 ALITGTFYGTKAEYE 
                   
               
               
                   
                   
                 ttgtttatcgctgaaccattgggcgccttaatcaccggc 
                   
                 ATGIAARLPSGGTLD 
                   
               
               
                   
                   
                 accttttacggaactaaagctgagtacgaagccacgg 
                   
                 LKLLDWLGSLAHIA 
                   
               
               
                   
                   
                 gtattgctgcaagattgccatccggtggtactcttgacc 
                   
                 EVVGLTLGDIPTSFY 
                   
               
               
                   
                   
                 taaagcttttggattggttgggttccttggcccacattgc 
                   
                 GKSLALREEDMLDR 
                   
               
               
                   
                   
                 tgaagttgtcggtcttactctaggtgacataccaacctc 
                   
                 TSIDGLFRYMGDAD 
                   
               
               
                   
                   
                 tttctatggtaagtcattggccttgagagaagaagatat 
                   
                 AGTLLWFVIFNSEG 
                   
               
               
                   
                   
                 gctagatagaacctcaatcgatggtttgttcagatacat 
                   
                 GAMADTPAGATAY 
                   
               
               
                   
                   
                 gggtgacgctgatgccggtaccttgttatggtttgtcatt 
                   
                 PHRDKLIMYQSYVI 
                   
               
               
                   
                   
                 tttaattcggaaggtggtgcaatggcagatacgccag 
                   
                 GIPTLTKATRDFADG 
                   
               
               
                   
                   
                 ctggcgcaactgcatatcctcatagagacaaactaatc 
                   
                 VHDRVRMGAPAAN 
                   
               
               
                   
                   
                 atgtaccaatcttatgttattggtatcccaactctgacaa 
                   
                 STYAGYIDRTLSREA 
                   
               
               
                   
                   
                 aggctaccagggacttcgctgatggtgttcacgacag 
                   
                 AQEFYWGAQLPRLR 
                   
               
               
                   
                   
                 agttagaatgggtgctccagctgctaacagtacttacg 
                   
                 EVKKAWDPKDVFH 
                   
               
               
                   
                   
                 ctggatacattgatagaaccttatctcgtgaagccgctc 
                   
                 NPQSVDPAE 
                   
               
               
                   
                   
                 aagaattttactggggtgcacaattgcctaggttgcgtg 
                   
                   
                   
               
               
                   
                   
                 aggtcaagaaggcttgggacccaaaggatgttttccat 
                   
                   
                   
               
               
                   
                   
                 aatccccaatccgtagacccagctgaa 
                   
                   
                   
               
               
                   
               
               
                 t808154 
                 Library 
                 atgggcaatactacgtctattgctgccggtagagactg 
                 89 
                 MGNTTSIAAGRDCLI 
                 159 
               
               
                   
                   
                 tcttatcagcgcagtcggtgctgctaacgtagcctttca 
                   
                 SAVGAANVAFQDQL 
                   
               
               
                   
                   
                 agatcagctgctataccaagctacagctgtgcaaccct 
                   
                 LYQATAVQPYNLNI 
                   
               
               
                   
                   
                 ataacttaaatatacctgttactccagctgccgttaccta 
                   
                 PVTPAAVTYPQSAD 
                   
               
               
                   
                   
                 cccacaaagtgccgacgagatcgctgccgttgtcaaa 
                   
                 EIAAVVKCASEYGY 
                   
               
               
                   
                   
                 tgcgcttcggaatatggttacaaggtccaagctaggtc 
                   
                 KVQARSGGHSFGNY 
                   
               
               
                   
                   
                 aggtggacactccttcggtaactacggtttgggtggcc 
                   
                 GLGGQDGAIVIEMK 
                   
               
               
                   
                   
                 aagatggtgcaattgttattgaaatgaagcatttctctca 
                   
                 HFSQFSMDESTFIATI 
                   
               
               
                   
                   
                 gttttctatggacgaatccaccttcatcgctactattggt 
                   
                 GPGITLGDLDTDLY 
                   
               
               
                   
                   
                 ccaggaatcaccttgggtgatttggatactgatttatata 
                   
                 NAGHRAMAHGICPT 
                   
               
               
                   
                   
                 acgccggtcacagagctatggctcatggtatatgtcca 
                   
                 IRTGGHLTVGGLGPT 
                   
               
               
                   
                   
                 accatcagaacgggtggtcacctaacagttggtggttt 
                   
                 ARQWGLALDHVEE 
                   
               
               
                   
                   
                 gggccctactgctcgtcaatggggcttagcattggac 
                   
                 VEVVLANSSIVRASD 
                   
               
               
                   
                   
                 catgtagaagaagtcgaagttgttctggctaactcttcc 
                   
                 TQNQDLFFAIKGAA 
                   
               
               
                   
                   
                 attgtccgtgcttctgacactcaaaatcaggatttgttttt 
                   
                 ASFGIVTEFKVRTEQ 
                   
               
               
                   
                   
                 cgctatcaagggtgccgccgcttccttcggtattgtaa 
                   
                 APGMAVQYSYTFHL 
                   
               
               
                   
                   
                 cagaatttaaagttagaaccgagcaagctccaggtat 
                   
                 GTSAEKAKFVKDW 
                   
               
               
                   
                   
                 ggcagtccaatacagttacaccttccaccttggtacttc 
                   
                 QAFIAQENLTWKFY 
                   
               
               
                   
                   
                 agctgaaaaggccaagttcgtcaaagactggcaagc 
                   
                 TNLVIFDDQIILEGIY 
                   
               
               
                   
                   
                 cttcattgctcaagaaaacttgacttggaagttttatacc 
                   
                 FGTKEEYDSLGLEQ 
                   
               
               
                   
                   
                 aacttggttatattcgatgatcaaatcatcttggaggga 
                   
                 RFPPTDAGTVLILTD 
                   
               
               
                   
                   
                 atatactttggtactaaagaagaatacgacagcttaggt 
                   
                 WLAMIGHGLEDTIL 
                   
               
               
                   
                   
                 cttgaacaaagattcccaccaactgacgcaggtactgt 
                   
                 KLVGDTPTWFYAKS 
                   
               
               
                   
                   
                 gttaattttgacagactggttggcaatgattggtcatgg 
                   
                 LGFTPRALIPDSAIDE 
                   
               
               
                   
                   
                 attggaggatacgattttaaagttggttggtgatacacc 
                   
                 FFDYIHENNPGTLA 
                   
               
               
                   
                   
                 cacctggttttatgccaagtctctaggtttcaccccaag 
                   
                 WFVTLSLEGGAINA 
                   
               
               
                   
                   
                 agctcttattccagatagcgctatcgacgaattttttgac 
                   
                 VPEDATAYGHRDVL 
                   
               
               
                   
                   
                 tacatacacgagaataaccctggtactttggcttggttc 
                   
                 FWFQLFVINPLGPIS 
                   
               
               
                   
                   
                 gtcacgttatctttggaaggaggtgctatcaacgctgtt 
                   
                 QTTYGFADGLYDVL 
                   
               
               
                   
                   
                 ccagaagatgcaaccgcttatggtcacagagatgtctt 
                   
                 AQAVPESASHAYMG 
                   
               
               
                   
                   
                 attctggttccaattgttcgttattaatcctttgggtccaat 
                   
                 CPDPRMPNAQRAY 
                   
               
               
                   
                   
                 ctcgcagactacttacggtttcgccgacggtctttacga 
                   
                 WRSNLPKLEELKGY 
                   
               
               
                   
                   
                 tgtcctggctcaagcagttcccgaatctgcttcgcatgc 
                   
                 LDPEDIFHNPQGVVP 
                   
               
               
                   
                   
                 atacatgggttgtccagatccaagaatgccaaacgctc 
                   
                 S 
                   
               
               
                   
                   
                 aacgtgcttactggagatccaacttgcctaaactggaa 
                   
                   
                   
               
               
                   
                   
                 gaactaaagggctatttggacccagaagacatttttca 
                   
                   
                   
               
               
                   
                   
                 caatccacaaggtgttgtaccctct 
                   
                   
                   
               
               
                   
               
               
                 t808155 
                 Library 
                 atgggtaacaccacatcaataactgctggccgtgattg 
                 90 
                 MGNTTSITAGRDCL 
                 160 
               
               
                   
                   
                 cctgacttccgccgtcggtggagttgctgcacatgtag 
                   
                 TSAVGGVAAHVAFQ 
                   
               
               
                   
                   
                 cttttcaagacgccttactatatcagaccccagctgtgg 
                   
                 DALLYQTPAVDPYN 
                   
               
               
                   
                   
                 acccttacaatttgaacattccagttacgcccgccgctg 
                   
                 LNIPVTPAAVTYPQS 
                   
               
               
                   
                   
                 ttacttacccacaaagcgctgatgaagtcgccgctgtc 
                   
                 ADEVAAVVKCASD 
                   
               
               
                   
                   
                 gttaagtgtgcttcggattataattacaaagttcaagcta 
                   
                 YNYKVQARSGGHSF 
                   
               
               
                   
                   
                 gatctggtggtcactccttcggtaacttcggtttgggtg 
                   
                 GNFGLGGQNGAIVV 
                   
               
               
                   
                   
                 gacaaaatggtgcaatcgtcgttgacatgaagcactttt 
                   
                 DMKHFSQFSMDEST 
                   
               
               
                   
                   
                 ctcaattctctatggatgagagtaccttcgtcgccactat 
                   
                 FVATIGPGTTLGNLD 
                   
               
               
                   
                   
                 tggtccaggcacaacccttggtaacttggacactgaa 
                   
                 TEIYNAGKRAMSHG 
                   
               
               
                   
                   
                 atctacaacgctggtaagagggctatgtctcatggtatt 
                   
                 ICPSIRTGGHLTVGG 
                   
               
               
                   
                   
                 tgtcctagtatcagaaccggtggtcacttgactgtagg 
                   
                 LGPTARQWGLALDH 
                   
               
               
                   
                   
                 cggtttaggtccaacagctagacaatggggtttggctc 
                   
                 VEEVEVVLANSSIIR 
                   
               
               
                   
                   
                 ttgaccacgttgaagaagtcgaagttgtgttggccaac 
                   
                 ASDTQNQDVLFAIK 
                   
               
               
                   
                   
                 tcatccattatcagagcttctgatacccagaaccaagat 
                   
                 GAAASFGIVTEFKVR 
                   
               
               
                   
                   
                 gtcctatttgcaattaaaggtgctgccgcatccttcgga 
                   
                 TEEAPGLAVQYSFTF 
                   
               
               
                   
                   
                 atagtaaccgaatttaaggttagaactgaagaggctcc 
                   
                 NLGTPAEKAKLVKD 
                   
               
               
                   
                   
                 aggcttagctgttcaatactccttcactttcaatctgggt 
                   
                 WQAYIAQENLTWKF 
                   
               
               
                   
                   
                 acgccagctgaaaaggcaaagttggtgaaagactgg 
                   
                 YSNLIIFDGQIILEGIF 
                   
               
               
                   
                   
                 caagcctatatcgcacaggaaaatttgacctggaagtt 
                   
                 FGSKEEYDQLNLDK 
                   
               
               
                   
                   
                 ttattctaaccttattatctttgacggtcaaattatcttgga 
                   
                 KFPTSEPGTVLVLTD 
                   
               
               
                   
                   
                 gggtattttctttggtagcaaggaagaatacgatcaatt 
                   
                 WLGMIGHGLEDTIL 
                   
               
               
                   
                   
                 aaacttagataagaaattccctacttccgaaccaggta 
                   
                 RLVGDSPTWFYAKS 
                   
               
               
                   
                   
                 cagttttggtattgactgactggttaggcatgattggtca 
                   
                 LGFTPSTLISGSAIDG 
                   
               
               
                   
                   
                 tggtttggaggacaccattctgcgtttagttggtgattct 
                   
                 LFDYIHKTNAGTLA 
                   
               
               
                   
                   
                 ccaacatggttttacgctaagtctttgggtttcacacctt 
                   
                 WFVTLSLEGGAINA 
                   
               
               
                   
                   
                 ctaccttgatatcaggcagtgctatcgacggtttgttcg 
                   
                 VPKDATAYGHRDVL 
                   
               
               
                   
                   
                 attacattcacaaaactaatgcaggaactctagcttggt 
                   
                 FWVQIFVANPLGPIS 
                   
               
               
                   
                   
                 ttgttacgttgagtttagaaggtggtgccataaacgctg 
                   
                 QTTYDFTDGLYDIL 
                   
               
               
                   
                   
                 tcccaaaggacgctactgcatatggtcatagagatgtc 
                   
                 AQAVPESAGHAYLG 
                   
               
               
                   
                   
                 ttgttctgggttcaaatcttcgtcgccaacccacttggtc 
                   
                 CPDPKMPDAQRAY 
                   
               
               
                   
                   
                 caatttcgcaaaccacttacgatttcaccgatggtcttta 
                   
                 WRSNLPRLEELKGD 
                   
               
               
                   
                   
                 cgacatcctggctcaggctgttcccgaatctgccggtc 
                   
                 LDPKDIFHNPQGVQ 
                   
               
               
                   
                   
                 acgcttatttgggttgtcccgatccaaagatgccagac 
                   
                 VAS 
                   
               
               
                   
                   
                 gctcaaagagcttattggagatccaatctgcctcgtttg 
                   
                   
                   
               
               
                   
                   
                 gaagaattgaagggtgatctggaccccaaggatatttt 
                   
                   
                   
               
               
                   
                   
                 ccataatccacaaggagttcaagtagcatca 
                   
                   
                   
               
               
                   
               
               
                 t808175 
                 Library 
                 atgaatccttcaattccatcttcctctatgggcaacacca 
                 91 
                 MNPSIPSSSMGNTTS 
                 161 
               
               
                   
                   
                 cttccatcgctggtagggattgtctggtcagcgccttag 
                   
                 IAGRDCLVSALGGN 
                   
               
               
                   
                   
                 gaggtaacgctggtttggtagcattccagaatcaacca 
                   
                 AGLVAFQNQPLYQT 
                   
               
               
                   
                   
                 ctataccaaacaactgctgtgcacgaatataacttgaa 
                   
                 TAVHEYNLNIPVTPA 
                   
               
               
                   
                   
                 cataccagtcacccccgccgctattacgtacccagag 
                   
                 AITYPETAEQIAAVV 
                   
               
               
                   
                   
                 actgctgaacaaatcgcagctgttgttaaatgcgccag 
                   
                 KCASQYDYKVQARS 
                   
               
               
                   
                   
                 tcaatatgactacaaggttcaagctagatcgggtggtc 
                   
                 GGHSFGNYGLGGTD 
                   
               
               
                   
                   
                 attcttttggtaattacggtttgggcggtacagacggtg 
                   
                 GAVVVDMKYFNQF 
                   
               
               
                   
                   
                 ccgttgtcgttgatatgaagtatttcaaccaattttctatg 
                   
                 SMDDQTYEAVIGPG 
                   
               
               
                   
                   
                 gacgatcagacttacgaagctgtcattggtcctggtac 
                   
                 TTLGDVDVELYNNG 
                   
               
               
                   
                   
                 cactttaggtgacgtcgatgtagaattgtacaataacg 
                   
                 KRAMAHGVCPTIST 
                   
               
               
                   
                   
                 gaaagagagctatggcccacggcgtttgtccaaccat 
                   
                 GGHFTMGGLGPTAR 
                   
               
               
                   
                   
                 ctccactggtggtcatttcacgatgggtggtcttggtcc 
                   
                 QWGLALDHVEEVE 
                   
               
               
                   
                   
                 aactgctcgtcaatggggtttggctttggatcacgtgg 
                   
                 VVLANSSIVRASNTQ 
                   
               
               
                   
                   
                 aggaagttgaagttgtcttagcaaattcatctattgttag 
                   
                 NQEVFFAVKGAAAS 
                   
               
               
                   
                   
                 agcaagcaacacacagaaccaagaagtcttctttgct 
                   
                 FGIVTEFKVRTQPAP 
                   
               
               
                   
                   
                 gtgaaaggcgctgccgcctcgttcggtatcgttactga 
                   
                 GIAVQYSYTFNLGSS 
                   
               
               
                   
                   
                 atttaaggtaagaacccaacccgctccaggaatagct 
                   
                 AEKAQFIKDWQSFV 
                   
               
               
                   
                   
                 gttcaatattcttacaccttcaacttaggttcttccgccga 
                   
                 SAKNLTRQFYTNMV 
                   
               
               
                   
                   
                 aaaagcccaattcattaaggactggcaatctttcgtatc 
                   
                 IFDGDIILEGLFFGSK 
                   
               
               
                   
                   
                 cgctaagaatttgaccagacaattttacacaaatatggt 
                   
                 EQYEALGLEERFVP 
                   
               
               
                   
                   
                 tatctttgacggtgatattattttggaaggtcttttcttcgg 
                   
                 KNPGNILVLTDWLG 
                   
               
               
                   
                   
                 ttccaaagaacaatatgaggctctgggtttggaagaaa 
                   
                 MVGHALEDTILRLV 
                   
               
               
                   
                   
                 gatttgtccctaagaacccaggcaacatcctggtcctg 
                   
                 GNTPTWFYAKSLGF 
                   
               
               
                   
                   
                 actgattggctaggtatggttggtcatgcattggaaga 
                   
                 TPDTLIPSSGIDEFFE 
                   
               
               
                   
                   
                 caccatactaagattagtcggcaacaccccaacctgg 
                   
                 YIENNKAGTSTWFV 
                   
               
               
                   
                   
                 ttttatgctaagtccttgggttttactcctgacactttgatt 
                   
                 TLSLEGGAINDVPAD 
                   
               
               
                   
                   
                 ccaagtagcggtatcgatgaatttttcgaatacatagaa 
                   
                 ATAYGHRDVLFWV 
                   
               
               
                   
                   
                 aataacaaggctggtacttccacctggttcgttactttg 
                   
                 QIFMVSPTGPVSSTT 
                   
               
               
                   
                   
                 agtcttgaaggtggtgctattaacgacgtcccagccga 
                   
                 YDFADGLYNVLTKA 
                   
               
               
                   
                   
                 tgctactgcttacggacaccgtgatgttctattctgggta 
                   
                 VPESEGHAYLGCPD 
                   
               
               
                   
                   
                 cagatcttcatggtttctcctacaggtccagttagttcta 
                   
                 PKMANAQQKYWRQ 
                   
               
               
                   
                   
                 cgacgtatgattttgctgatggtttgtacaatgtgttgac 
                   
                 NLPRLEELKETLDPK 
                   
               
               
                   
                   
                 caaagctgttccagaatcagaaggtcacgcttatttag 
                   
                 DTFHNPQGILPA 
                   
               
               
                   
                   
                 gatgtccagacccaaagatggccaacgcccaacaaa 
                   
                   
                   
               
               
                   
                   
                 agtattggagacaaaacttgccaagattggaggagtta 
                   
                   
                   
               
               
                   
                   
                 aaggagacattggatcctaaagacactttccataatcc 
                   
                   
                   
               
               
                   
                   
                 ccaaggaatcctaccagcc 
                   
                   
                   
               
               
                   
               
               
                 t808177 
                 Library 
                 atgggtaacacaaccagtatagccggacgtgattgctt 
                 92 
                 MGNTTSIAGRDCLIS 
                 162 
               
               
                   
                   
                 gatttcagcacttggtggcaattccgctctagctgttttc 
                   
                 ALGGNSALAVFPNE 
                   
               
               
                   
                   
                 ccaaacgagttgctgtggacggctgacgtgcacgaat 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                   
                 ataacttaaatttgcccgtaactccagccgctattaccta 
                   
                 PVTPAAITYPETAAQ 
                   
               
               
                   
                   
                 ccctgaaactgctgcacaaatcgctggtgttgtcaaat 
                   
                 IAGVVKCASDYDYK 
                   
               
               
                   
                   
                 gtgcttctgactacgattataaggttcaggccagatctg 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 gtggtcattcgtttggtaactacggtttgggaggtgcag 
                   
                 LGGADGAVVVDMK 
                   
               
               
                   
                   
                 atggcgctgtcgttgtggacatgaagcacttcactcaa 
                   
                 HFTQFSMDDETYEA 
                   
               
               
                   
                   
                 ttctcaatggatgacgaaacctacgaagctgttattggt 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 ccaggtactacattaaatgacgtcgatatcgaattatat 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 aacaacggtaagagagccatggctcatggtgtctgtc 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 caaccatcaaaactggtggtcactttaccatcggtggtt 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 tgggtcctactgctaggcaatggggcctagccttggat 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 catgtcgaagaagttgaagttgttttggctaattcttcca 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 ttgttagagcttctaacactcaaaatcaagacgtattcttt 
                   
                 AANFGIVTEFKVRTE 
                   
               
               
                   
                   
                 gccgtcaagggtgccgctgctaattttggaattgtaac 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 agagttcaaggtcagaactgaaccagcaccaggttta 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 gctgttcaatacagctacaccttcaacttgggatccacc 
                   
                 QSFISAKNLTRQFYN 
                   
               
               
                   
                   
                 gcagaaaaagctcagttcgtgaaggactggcaatcttt 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 tatctccgctaaaaaccttacgcgtcaattctataacaa 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 catggtcatattcgatggtgatattatattggagggtctg 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 ttttttggtagtaaagaacaatacgacgctttgggtttgg 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 aagatcacttcgcaccaaagaaccccggcaatatctt 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 ggttttaactgactggcttggcatggttggtcacgcttta 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 gaagacacaattttgaagttggtcggtaatactccaac 
                   
                 EFFEYIANHTAGTPA 
                   
               
               
                   
                   
                 ctggttctatgccaagtctttaggttttagacaagatact 
                   
                 WFVTLSLEGGAIND 
                   
               
               
                   
                   
                 ctaattcctagtgccggaatcgatgaatttttcgaataca 
                   
                 VAEDATAYAHRDV 
                   
               
               
                   
                   
                 ttgctaatcatactgctggtactccagcatggttcgttac 
                   
                 LFWVQLFMVNPLGP 
                   
               
               
                   
                   
                 gttgtccttagaaggtggtgctataaacgatgtcgccg 
                   
                 ISDTTYEFTDGLYDV 
                   
               
               
                   
                   
                 aagatgctactgcctacgctcacagggacgttttgttct 
                   
                 LARAVPESVGHAYL 
                   
               
               
                   
                   
                 gggtacaattgtttatggtcaatccattgggtcccatctc 
                   
                 GCPDPRMEDAQQK 
                   
               
               
                   
                   
                 tgacaccacgtatgagtttaccgacggtctgtacgatg 
                   
                 YWRTNLPRLQELKE 
                   
               
               
                   
                   
                 ttctagctagagctgtgccagaatctgttggtcatgcct 
                   
                 ELDPKNTFHHPQGV 
                   
               
               
                   
                   
                 atttgggttgtccagaccctagaatggaagatgcccaa 
                   
                 MPA 
                   
               
               
                   
                   
                 cagaagtactggagaaccaaccttccaagattacaag 
                   
                   
                   
               
               
                   
                   
                 aattgaaggaagaactagatccaaagaatacatttcat 
                   
                   
                   
               
               
                   
                   
                 caccctcaaggtgtaatgcctgct 
                   
                   
                   
               
               
                   
               
               
                 t808199 
                 Library 
                 atgggaaacacaacgtccatagctgccggtagagact 
                 93 
                 MGNTTSIAAGRDCL 
                 163 
               
               
                   
                   
                 gcctattatcggcagtaggcggtaatcacgctcatgtc 
                   
                 LSAVGGNHAHVAFQ 
                   
               
               
                   
                   
                 gctttccaagatcagcttttgtatcaagtgaccgctgttg 
                   
                 DQLLYQVTAVEPYN 
                   
               
               
                   
                   
                 agccttacaacttgaatattccagttacccccgccgctg 
                   
                 LNIPVTPAAVTYPQS 
                   
               
               
                   
                   
                 ttacttacccacaatcagccgacgaaatcgctgccgtc 
                   
                 ADEIAAVVKCASEY 
                   
               
               
                   
                   
                 gtcaaatgtgcttctgaatatggttacaaggttcaagct 
                   
                 GYKVQARSGGHSFG 
                   
               
               
                   
                   
                 aggtctggtggtcactcctttggtaactacggtctgggt 
                   
                 NYGLGGEDGAIVVE 
                   
               
               
                   
                   
                 ggtgaagatggcgctattgttgtggaaatgaagcattt 
                   
                 MKHFNQFSMDESTY 
                   
               
               
                   
                   
                 caatcaatttagtatggatgaatctacttatactgcaact 
                   
                 TATIGPGITLGDLDT 
                   
               
               
                   
                   
                 atcggtccaggaattaccttgggtgacttggacaccgc 
                   
                 ALYNAGHRAMAHG 
                   
               
               
                   
                   
                 tttatacaacgctggtcacagagccatggcacatggta 
                   
                 ICPTIRTGGHLTMGG 
                   
               
               
                   
                   
                 tctgtccaaccatacgtactggtggccacttgaccatg 
                   
                 LGPTARQWGLALDH 
                   
               
               
                   
                   
                 ggtggtctgggtcctacagctagacaatggggtttagc 
                   
                 VEEVEVVLANSSIVR 
                   
               
               
                   
                   
                 attagatcatgtcgaagaggtcgaagttgttttggctaa 
                   
                 ASNTQNQDILFAIKG 
                   
               
               
                   
                   
                 cagctctattgtcagagccagtaacacacagaatcaa 
                   
                 AAASFGIVTEFKVRT 
                   
               
               
                   
                   
                 gatattttgttcgctatcaagggtgccgctgcttccttcg 
                   
                 EAAPGVAVQYSFTF 
                   
               
               
                   
                   
                 gtattgttactgagtttaaagtaagaactgaagccgctc 
                   
                 NLGTPAEKAKLVKD 
                   
               
               
                   
                   
                 caggtgttgcagtccaatactccttcacttttaacctagg 
                   
                 WQAFIAQEDLTWKF 
                   
               
               
                   
                   
                 aacgccagctgaaaaggcaaagcttgttaaagactgg 
                   
                 YSNMNIFDGQIILEGI 
                   
               
               
                   
                   
                 caagccttcatcgctcaagaagatttgacttggaagttc 
                   
                 YFGSKEEYDALGLE 
                   
               
               
                   
                   
                 tattctaacatgaatatatttgacggccaaatcattttgg 
                   
                 KRFPSSEAGTVLVLT 
                   
               
               
                   
                   
                 aaggtatctacttcggtagtaaggaagagtacgatgct 
                   
                 DWLGMVGHGLEDV 
                   
               
               
                   
                   
                 ttaggtttagaaaagagatttccctcatctgaagctggt 
                   
                 ILRLVGNTPTWFYA 
                   
               
               
                   
                   
                 accgtgttggttttgaccgattggttgggtatggtcggc 
                   
                 KSLGFTPRALIPDSAI 
                   
               
               
                   
                   
                 cacggtctggaagatgtgattctaagattggttggtaac 
                   
                 DEFLNYIHENTPGTV 
                   
               
               
                   
                   
                 accccaacttggttctacgcaaaatcattgggattcact 
                   
                 SWFVTLSLEGGAIN 
                   
               
               
                   
                   
                 ccaagagctttgatacctgactcagctattgacgaattt 
                   
                 KVPGDATAYGHRD 
                   
               
               
                   
                   
                 cttaattacatccacgaaaacacgcctggtacagtatc 
                   
                 VLFWVQIFMINPLGP 
                   
               
               
                   
                   
                 ctggttcgtcactctatctttggaaggtggtgccattaac 
                   
                 VSQTTYGFADGLYD 
                   
               
               
                   
                   
                 aaggtcccaggcgatgctactgcctatggccaccgtg 
                   
                 VLAKAVPNSAGHAY 
                   
               
               
                   
                   
                 atgtgttattctgggttcagatttttatgatcaacccattg 
                   
                 LGCPDPRMPNAQQA 
                   
               
               
                   
                   
                 ggtccagtttctcaaaccacttatggtttcgctgacgga 
                   
                 YWRSNLPRLEELKG 
                   
               
               
                   
                   
                 ttatatgacgttttggcaaaggctgtaccaaactcggct 
                   
                 ELDPKDIFHNPQGV 
                   
               
               
                   
                   
                 ggacacgcctacttaggttgtcccgatccaagaatgc 
                   
                 MVVS 
                   
               
               
                   
                   
                 caaatgctcaacaagcttattggaggtctaatttgccca 
                   
                   
                   
               
               
                   
                   
                 gattggaggaattgaagggtgaactggatccaaaaga 
                   
                   
                   
               
               
                   
                   
                 catttttcataacccacaaggtgttatggttgtctcc 
                   
                   
                   
               
               
                   
               
               
                 t808200 
                 Library 
                 atgggcaatacgacatccattgcaggtagagattgtct 
                 94 
                 MGNTTSIAGRDCLIS 
                 164 
               
               
                   
                   
                 tataagcgccctaggtggaaactcggctttggctgcttt 
                   
                 ALGGNSALAAFPNE 
                   
               
               
                   
                   
                 ccctaacgagttactgtggactgctgacgtccatgaat 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                   
                 acaatttgaacttgcccgttactccagccgctatcacct 
                   
                 PVTPAAITYPETAEQ 
                   
               
               
                   
                   
                 atccagaaaccgctgaacaaatcgctggtattgtgaaa 
                   
                 IAGIVKCASDYDYK 
                   
               
               
                   
                   
                 tgcgcctctgattacgactataaggttcaggcacgttct 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 ggtggtcactcatttggtaattacggtttgggtggtgcc 
                   
                 LGGADGAVVVDMK 
                   
               
               
                   
                   
                 gatggagctgttgtagtcgacatgaagcacttcactca 
                   
                 HFTQFSMDDETYEA 
                   
               
               
                   
                   
                 atttagtatggatgacgaaacctacgaagctgtcatcg 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 gtccaggtacaactttaaacgacgttgatattgaattata 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 taacaatggcaaaagagccatggcacatggtgtttgtc 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 caactatcaagaccggaggtcacttcaccattggtggt 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 ttgggtcctacagctagacaatggggtttggctctgga 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 ccacgtcgaggaagtagaagttgtcttggcaaactctt 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 ccattgtgagggcctctaacactcaaaatcaagatgttt 
                   
                 AANFGIVTEFKVRTE 
                   
               
               
                   
                   
                 tctttgcagttaagggtgctgctgctaacttcggtatagt 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 gaccgagtttaaagttagaacggaaccagctccaggc 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 ttagctgtccagtactcctatactttcaacttgggttcaa 
                   
                 QSFISAKNLTRQFYN 
                   
               
               
                   
                   
                 ctgctgaaaaggctcaattcgttaaggattggcaatcat 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 tcatctctgctaagaatcttactagacaattttacaacaa 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 catggtcatttttgacggtgatatcattttagaaggtttatt 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 tttcggcagtaaggaacaatacgacgccttgggtttgg 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 aagatcattttgcaccaaagaaccctggtaacattttgg 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 tactaaccgactggttgggaatggttggtcacgcccta 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 gaagatacaatattgaaattggttggcaatactccaac 
                   
                 EFFEYIANHTAGTPA 
                   
               
               
                   
                   
                 ctggttctacgctaaatctttgggtttcagacaggatac 
                   
                 WFVTLSLEGGAINDI 
                   
               
               
                   
                   
                 cttgattccatccgctggtatcgacgaatttttcgaatat 
                   
                 AEDATAYAHRDVLF 
                   
               
               
                   
                   
                 attgctaatcatactgctggtaccccagcttggttcgtc 
                   
                 WVQLFMVNPLGPIS 
                   
               
               
                   
                   
                 accttaagcctagagggtggtgccatcaatgatatcgc 
                   
                 DTTYEFTDGLYDVL 
                   
               
               
                   
                   
                 tgaagacgctactgcctacgcacatagagatgtcttatt 
                   
                 ARAVPESVGHAYLG 
                   
               
               
                   
                   
                 ctgggtccaactgtttatggttaaccctttgggtcccata 
                   
                 CPDPRMEDAQQKY 
                   
               
               
                   
                   
                 tctgatacaacttacgaatttacagacggtctgtatgac 
                   
                 WRTNLPRLQELKEE 
                   
               
               
                   
                   
                 gttctagcacgtgctgtaccagagtctgtcggccacgc 
                   
                 LDPKNTFHHPQGVM 
                   
               
               
                   
                   
                 ttacttaggctgtcccgacccaagaatggaagacgca 
                   
                 PA 
                   
               
               
                   
                   
                 caacaaaagtattggagaaccaacctaccaagattgc 
                   
                   
                   
               
               
                   
                   
                 aagaattgaaggaagagttggacccaaagaacacgtt 
                   
                   
                   
               
               
                   
                   
                 tcaccatccacagggtgttatgcctgca 
                   
                   
                   
               
               
                   
               
               
                 t808223 
                 Library 
                 atgggtaatacgacttccatagccggaagggactgcc 
                 95 
                 MGNTTSIAGRDCLIS 
                 165 
               
               
                   
                   
                 taatctctgctttgggtggtaactcggctctggcagtctt 
                   
                 ALGGNSALAVFPNE 
                   
               
               
                   
                   
                 ccctaacgagttattgtggaccgctgatgttcacgaata 
                   
                 LLWTADVHEYNLNL 
                   
               
               
                   
                   
                 caatttgaacttgccagttactccagccgctattacctat 
                   
                 PVTPAAITYPETAAQ 
                   
               
               
                   
                   
                 cccgaaacagctgcacagattgctggcgtagtcaaat 
                   
                 IAGVVKCASDYDYK 
                   
               
               
                   
                   
                 gtgcctcagattacgactacaaggtgcaagctagatct 
                   
                 VQARSGGHSFGNYG 
                   
               
               
                   
                   
                 ggtggtcatagctttggtaactatggtttaggaggtgct 
                   
                 LGGADGAVVVDMK 
                   
               
               
                   
                   
                 gatggcgcagttgttgtcgacatgaagcacttcactca 
                   
                 HFTQFSMDDETYEA 
                   
               
               
                   
                   
                 atttagtatggatgacgaaacttacgaagctgttatcgg 
                   
                 VIGPGTTLNDVDIEL 
                   
               
               
                   
                   
                 tccaggtaccaccctaaatgatgttgatatcgaattgtat 
                   
                 YNNGKRAMAHGVC 
                   
               
               
                   
                   
                 aacaatggtaagagagctatggcacatggtgtttgtcc 
                   
                 PTIKTGGHFTIGGLG 
                   
               
               
                   
                   
                 aacaattaaaactggaggtcacttcaccattggcggttt 
                   
                 PTARQWGLALDHVE 
                   
               
               
                   
                   
                 aggtcctactgccagacaatggggtcttgctttggacc 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 atgtcgaagaagtagaggtcgttcttgctaactcttctat 
                   
                 NTQNQDVFFAVKGA 
                   
               
               
                   
                   
                 cgttcgtgcttccaacactcaaaaccaagatgtgttcttt 
                   
                 AANFGIVTEFKVRTE 
                   
               
               
                   
                   
                 gccgtcaagggtgctgctgccaacttcggtattgtaac 
                   
                 PAPGLAVQYSYTFN 
                   
               
               
                   
                   
                 agaatttaaagttagaactgaaccagctccaggtttag 
                   
                 LGSTAEKAQFVKDW 
                   
               
               
                   
                   
                 ccgtccagtactcttataccttcaatttgggttccacggc 
                   
                 QSFISAKNLTRQFYN 
                   
               
               
                   
                   
                 tgaaaaggctcaattcgttaaggactggcaatccttcat 
                   
                 NMVIFDGDIILEGLF 
                   
               
               
                   
                   
                 atctgccaagaatttgaccagacaattttacaataacat 
                   
                 FGSKEQYDALGLED 
                   
               
               
                   
                   
                 ggttatctttgacggagatattatattggagggtctatttt 
                   
                 HFAPKNPGNILVLTD 
                   
               
               
                   
                   
                 tcggtagtaaggaacaatacgacgctctgggcttaga 
                   
                 WLGMVGHALEDTIL 
                   
               
               
                   
                   
                 agatcactttgctccaaaaaacccaggtaatatcttggt 
                   
                 KLVGNTPTWFYAKS 
                   
               
               
                   
                   
                 attgaccgattggttgggtatggtcggtcatgcccttga 
                   
                 LGFRQDTLIPSAGID 
                   
               
               
                   
                   
                 agatacaattttgaagctggttggtaacactccaacttg 
                   
                 EFFEYIANHTAGTPA 
                   
               
               
                   
                   
                 gttctacgcaaagtccttaggtttccgtcaagacacgtt 
                   
                 WFVTLSLEGGAIND 
                   
               
               
                   
                   
                 aattccttcagccggcatcgatgaatttttcgaatacatc 
                   
                 VAEDATAYAHRDV 
                   
               
               
                   
                   
                 gctaaccacaccgctggtactcctgcttggttcgtcac 
                   
                 LFWVQLFMVNPVGP 
                   
               
               
                   
                   
                 cttgagcttggaaggcggtgccattaacgatgtcgcc 
                   
                 ISDTTYEFTDGLYDV 
                   
               
               
                   
                   
                 gaggacgcaacggcttacgctcacagagatgttttgtt 
                   
                 LARAVPESVGHAYL 
                   
               
               
                   
                   
                 ctgggtccaattattcatggtgaatccagtgggtcctat 
                   
                 GCPDPRMEDAQQK 
                   
               
               
                   
                   
                 atctgacactacttatgaatttactgatggtttgtacgac 
                   
                 YWRTNLPRLQELKE 
                   
               
               
                   
                   
                 gttctagctagagcagtccctgagagcgtgggtcatg 
                   
                 ELDPKNTFHHPQGV 
                   
               
               
                   
                   
                 cttatttgggttgtccagacccaagaatggaagatgcc 
                   
                 MPA 
                   
               
               
                   
                   
                 caacagaaatattggaggacaaatttacccagattgca 
                   
                   
                   
               
               
                   
                   
                 agaattaaaagaggaattggatccaaagaacacattc 
                   
                   
                   
               
               
                   
                   
                 caccatccacagggtgttatgcccgct 
                   
                   
                   
               
               
                   
               
               
                 t808225 
                 Library 
                 atgggcaatacaacgtccattgccgctggtcgtgactg 
                 96 
                 MGNTTSIAAGRDCLI 
                 166 
               
               
                   
                   
                 cttgatcagcgctgttggaggtaacgcagctcacgtg 
                   
                 SAVGGNAAHVAFQ 
                   
               
               
                   
                   
                 gcctttcaggatcaacttttatatcaagctaccgcagtc 
                   
                 DQLLYQATAVDVY 
                   
               
               
                   
                   
                 gatgtttacaacttgaacatacccgtcactccagctgcc 
                   
                 NLNIPVTPAAVTYPQ 
                   
               
               
                   
                   
                 gtaacttaccctcaatcagctgacgaggttgctgctgtt 
                   
                 SADEVAAVVKCASE 
                   
               
               
                   
                   
                 gtcaagtgtgcctcggaatacgattataaagtccaagc 
                   
                 YDYKVQARSGGHSF 
                   
               
               
                   
                   
                 tagatctggtggtcattctttcggtaattacggtctaggt 
                   
                 GNYGLGGQNGAIVV 
                   
               
               
                   
                   
                 ggtcaaaatggagctattgttgtcgacatgaagcactt 
                   
                 DMKHFSQFSMDEST 
                   
               
               
                   
                   
                 cagtcaatttagtatggacgaatcaacctatactgcaac 
                   
                 YTATIGPGITLGDLD 
                   
               
               
                   
                   
                 catcggcccaggtatcactctgggtgatttagataccg 
                   
                 TELYNAGHRAMAH 
                   
               
               
                   
                   
                 aattgtacaacgctggtcatagagcaatggctcacggt 
                   
                 GICPTIRTGGHLTIGG 
                   
               
               
                   
                   
                 atttgtccaacaataagaactggtggtcacttgactatc 
                   
                 LGPTARQWGLALDH 
                   
               
               
                   
                   
                 ggtggtttgggtccaacagccaggcagtggggtctg 
                   
                 VEEVEVVLANSSIVR 
                   
               
               
                   
                   
                 gctttagaccatgttgaagaggtagaagttgtgttggct 
                   
                 ASETQNQDVLFAVK 
                   
               
               
                   
                   
                 aactcttccattgttagagcctctgaaacgcaaaacca 
                   
                 GAAASFGIVTEFKVR 
                   
               
               
                   
                   
                 agatgtcttgttcgcagtaaagggcgctgctgcttcctt 
                   
                 TEQAPGLAVQYSYT 
                   
               
               
                   
                   
                 tggtattgttaccgaatttaaagttagaactgaacaagc 
                   
                 FNLGTPAEKAKLLK 
                   
               
               
                   
                   
                 tcctggcctagctgtccagtattcctacaccttcaatttg 
                   
                 DWQAFIAQEDLTWK 
                   
               
               
                   
                   
                 ggtaccccagctgagaaggccaagttattaaaagact 
                   
                 FYSNMVIFDGQIILE 
                   
               
               
                   
                   
                 ggcaagctttcatcgcccaagaagacttgacctggaa 
                   
                 GIFFGSKEEYDALDL 
                   
               
               
                   
                   
                 gttctactccaatatggttattttcgatggtcaaatcatttt 
                   
                 EKRFPTSEPGTLLVL 
                   
               
               
                   
                   
                 ggaaggaattttctttggttctaaggaagaatatgatgc 
                   
                 TDWLGMVGHSLED 
                   
               
               
                   
                   
                 cctggatcttgagaagagatttccaacttctgaacctgg 
                   
                 VILRLVGNTPTWFY 
                   
               
               
                   
                   
                 tactttgttggttttaacggactggcttggtatggtaggt 
                   
                 AKSLGFTPRTLIPDS 
                   
               
               
                   
                   
                 catagcctggaagacgtcatattaaggctagttggtaa 
                   
                 AIDRFFDYIHETNAG 
                   
               
               
                   
                   
                 caccccaacttggttttacgctaagtctttgggcttcact 
                   
                 TLAWFVTLSLEGGAI 
                   
               
               
                   
                   
                 ccaagaaccttgatccctgacagcgctatagatagatt 
                   
                 NAVPEDATAYGHRD 
                   
               
               
                   
                   
                 cttcgactatattcacgaaactaacgctggtaccttggc 
                   
                 VLFWVQIFMVNPLG 
                   
               
               
                   
                   
                 atggtttgtgacgctttcattggaaggtggtgctattaat 
                   
                 PISQTIYDFADGLYD 
                   
               
               
                   
                   
                 gccgtgccagaagatgcaaccgcctacggtcatcgt 
                   
                 VLAQAVPESAEHAY 
                   
               
               
                   
                   
                 gatgttttgttttgggttcaaatcttcatggtcaacccctt 
                   
                 LGCPDPKMPDAQRA 
                   
               
               
                   
                   
                 gggaccaatttctcaaactatctacgatttcgctgacgg 
                   
                 YWRGNLPRLEELKG 
                   
               
               
                   
                   
                 actatacgacgtgttggcacaagccgtaccagaatcg 
                   
                 EFDPKDTFHNPQGV 
                   
               
               
                   
                   
                 gctgaacacgcttacttaggatgtccagatcctaaaat 
                   
                 SVAV 
                   
               
               
                   
                   
                 gccagacgcccaacgtgcttattggagaggtaactta 
                   
                   
                   
               
               
                   
                   
                 ccaagactggaggaattgaaaggagagtttgatccca 
                   
                   
                   
               
               
                   
                   
                 aggacacatttcacaacccacagggtgtttctgtcgcc 
                   
                   
                   
               
               
                   
                   
                 gtc 
                   
                   
                   
               
               
                   
               
               
                 t808226 
                 Library 
                 atgggcaacaccacgagcatcgctgccggtagagat 
                 97 
                 MGNTTSIAAGRDCLI 
                 167 
               
               
                   
                   
                 tgtttaatatctgctgttggaggtaatgcagctcacgtc 
                   
                 SAVGGNAAHVAFQ 
                   
               
               
                   
                   
                 gcctttcaggaccaactgctttaccaagctactgctgtg 
                   
                 DQLLYQATAVEPYN 
                   
               
               
                   
                   
                 gaaccttataacctaaatattccaatcaccccagccgct 
                   
                 LNIPITPAAITYPQSA 
                   
               
               
                   
                   
                 attacatacccccaatcggctgatgagatcgcagcagt 
                   
                 DEIAAVVKCASEYG 
                   
               
               
                   
                   
                 tgtaaagtgcgcttcagaatatggttacaaagtccaag 
                   
                 YKVQARSGGHSFGN 
                   
               
               
                   
                   
                 ctcgttccggtggtcattctttcggtaactacggtttagg 
                   
                 YGLGGEDGAIVVEM 
                   
               
               
                   
                   
                 tggtgaagacggtgctattgttgtcgaaatgaagcactt 
                   
                 KHFSQFSMDESTYIA 
                   
               
               
                   
                   
                 cagtcaattttccatggatgaatctacttatattgccacta 
                   
                 TIGPGITLGDLDTEL 
                   
               
               
                   
                   
                 tcggcccaggtattacattgggagacttggataccgaa 
                   
                 YNVGHRAMAHGICP 
                   
               
               
                   
                   
                 ttatacaatgttggtcatagagctatggcccacggtatc 
                   
                 TIRTGGHLTVGGLGP 
                   
               
               
                   
                   
                 tgtccaactattagaaccggtggtcatttgactgttgga 
                   
                 TARQWGLALDHVE 
                   
               
               
                   
                   
                 ggtttgggtcctaccgctaggcaatggggcctggcctt 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 ggatcacgttgaggaagtcgaagtcgtattggctaact 
                   
                 DTQNQDIFFAIKGAA 
                   
               
               
                   
                   
                 cttccatagttagagcatcagacactcagaaccaaga 
                   
                 ASFGIVTEFKVRTEQ 
                   
               
               
                   
                   
                 catcttcttcgctattaaaggtgctgctgctagctttggt 
                   
                 APGLAVQYSYTFNL 
                   
               
               
                   
                   
                 atagtgacagaatttaaggttagaaccgagcaagccc 
                   
                 GTPAEKAKLVKDW 
                   
               
               
                   
                   
                 caggtctagccgtgcaatactcttacactttcaacttgg 
                   
                 QAFIAQENLSWKFY 
                   
               
               
                   
                   
                 gtacaccagctgaaaaggccaagttggttaaggactg 
                   
                 SNMVVFDGQIILEGL 
                   
               
               
                   
                   
                 gcaggctttcattgctcaagaaaatctgtcatggaaatt 
                   
                 YFGSKEEYDALGLE 
                   
               
               
                   
                   
                 ctactctaatatggtcgtattcgatggccaaatcatctta 
                   
                 QRFPPSEAGNVLVLT 
                   
               
               
                   
                   
                 gaaggtttgtactttggctccaaggaagaatatgatgct 
                   
                 DWLGMVGHELEDTI 
                   
               
               
                   
                   
                 cttggtcttgaacaacgtttccccccatctgaagctggt 
                   
                 LRLVGNTPTWFYAK 
                   
               
               
                   
                   
                 aacgttctagtcttgactgattggttgggtatggttggtc 
                   
                 SLGFTPRALIPDSAID 
                   
               
               
                   
                   
                 atgagttagaagatactattttgagattggtaggtaaca 
                   
                 DLFNYIHENNPGTLA 
                   
               
               
                   
                   
                 cccctacttggttctacgctaaaagcttgggatttaccc 
                   
                 WFVTLSLEGGAINT 
                   
               
               
                   
                   
                 caagagccctgattccagactccgcaatagatgactta 
                   
                 VPEHATAYGHRDVL 
                   
               
               
                   
                   
                 ttcaactatattcacgagaataacccaggtaccttggca 
                   
                 FWVQIFVINPLGPVS 
                   
               
               
                   
                   
                 tggttcgtcacactttctttagaaggtggtgcaatcaac 
                   
                 QTTYGFADGMYDV 
                   
               
               
                   
                   
                 accgttcctgaacacgctactgcctatggacatagaga 
                   
                 LAQAVPESAGHAYL 
                   
               
               
                   
                   
                 tgttttgttttgggtccaaatttttgttatcaatccattgggt 
                   
                 GCPDPRMPNAQQAY 
                   
               
               
                   
                   
                 cccgtcagccaaacgacttacggttttgctgatggtat 
                   
                 WRSNLPRLEELKGD 
                   
               
               
                   
                   
                 gtatgacgtgcttgcccaagctgttccagaaagtgctg 
                   
                 LDPKGIFHNPQGVM 
                   
               
               
                   
                   
                 gtcatgcttacttgggttgtccagatccacgtatgccaa 
                   
                 VVS 
                   
               
               
                   
                   
                 acgcccaacaagcttactggagatctaatttgcctaga 
                   
                   
                   
               
               
                   
                   
                 ttagaagaattgaagggcgacctagacccaaaaggta 
                   
                   
                   
               
               
                   
                   
                 tcttccacaatccacaaggtgttatggtagtctcc 
                   
                   
                   
               
               
                   
               
               
                 t808232 
                 Library 
                 atgggtaacactacgtcgatcgcagctggacgtgatt 
                 98 
                 MGNTTSIAAGRDCL 
                 168 
               
               
                   
                   
                 gcctattgtccgctgttggtggcaatcatgcccacgta 
                   
                 LSAVGGNHAHVAFQ 
                   
               
               
                   
                   
                 gctttccaggaccaacttttgtatcaagccacagctgtc 
                   
                 DQLLYQATAVEPYN 
                   
               
               
                   
                   
                 gaaccatacaacttaaacatacctgtgactccagctgc 
                   
                 LNIPVTPAAVTYPQS 
                   
               
               
                   
                   
                 cgttacctacccccaatctgctgatgaggtcgcagctg 
                   
                 ADEVAAVVKCAAD 
                   
               
               
                   
                   
                 ttgttaagtgtgctgccgactatggttacaaagtccaag 
                   
                 YGYKVQARSGGHSF 
                   
               
               
                   
                   
                 ctagatcaggtggtcacagttttggtaattacggtttgg 
                   
                 GNYGLGGEDGAIVV 
                   
               
               
                   
                   
                 gtggtgaagacggtgctattgttgtagatatgaagcatt 
                   
                 DMKHFDQFSMDEST 
                   
               
               
                   
                   
                 tcgatcaatttagcatggatgaatctacctacactgcca 
                   
                 YTATIGPGITLGDLD 
                   
               
               
                   
                   
                 ccatcggcccaggtattactctgggcgacttggatacc 
                   
                 TALYNAGHRAMAH 
                   
               
               
                   
                   
                 gctttatataatgccggtcacagagctatggcacatgg 
                   
                 GICPTIRTGGHLTIGG 
                   
               
               
                   
                   
                 tatctgtccaactattagaacaggcggtcacttgaccat 
                   
                 LGPTARQWGLALDH 
                   
               
               
                   
                   
                 tggtggtttgggtcctacggctaggcaatggggattgg 
                   
                 VEEVEVVLANSSIVR 
                   
               
               
                   
                   
                 cactagaccacgtcgaagaagttgaggttgtcctggct 
                   
                 ASDTQNQEILFAVK 
                   
               
               
                   
                   
                 aactcctctatagtcagagcctctgacactcagaacca 
                   
                 GAAASFGIVTEFKVR 
                   
               
               
                   
                   
                 agaaattttattcgctgttaagggtgctgccgcttccttc 
                   
                 TEEAPGLAVQYSFTF 
                   
               
               
                   
                   
                 ggtatcgtcactgaatttaaagttagaaccgaagaagc 
                   
                 NLGTAAEKAKLVKD 
                   
               
               
                   
                   
                 tccaggattggcagtccaatacagcttcaccttcaacct 
                   
                 WQAFIAQEDLTWKF 
                   
               
               
                   
                   
                 tggtactgccgctgaaaaggctaagttggtgaaagatt 
                   
                 YSNMNIIDGQIILEGI 
                   
               
               
                   
                   
                 ggcaagcttttatcgcccaggaagacttaacgtggaa 
                   
                 YFGSKAEYDALGLE 
                   
               
               
                   
                   
                 gttttattctaacatgaacattatcgatggtcaaattattct 
                   
                 EKFPTSEPGTVLVLT 
                   
               
               
                   
                   
                 ggagggtatctacttcggttcgaaagctgaatacgac 
                   
                 DWLGMVGHGLEDV 
                   
               
               
                   
                   
                 gcattgggattggaagagaagtttccaacatcagaac 
                   
                 ILRLVGNAPTWFYA 
                   
               
               
                   
                   
                 ccggtactgtgcttgtattaactgactggttgggtatggt 
                   
                 KSLGFAPRALIPDSAI 
                   
               
               
                   
                   
                 tggtcacggtttagaagatgttattttgcgtttggttgga 
                   
                 DDFFEYIHKNNPGT 
                   
               
               
                   
                   
                 aatgctccaacttggttttatgcaaagtcactaggtttcg 
                   
                 VSWFVTLSLEGGAI 
                   
               
               
                   
                   
                 ctccaagagctttaatacctgatagtgcaattgatgactt 
                   
                 NKVPEDATAYGHRD 
                   
               
               
                   
                   
                 cttcgaatatatccataagaataacccaggtacagtctc 
                   
                 VLFWVQIFMINPLGP 
                   
               
               
                   
                   
                 ttggttcgtcaccttgtccttggagggtggtgccatcaa 
                   
                 VSQTIYDFADGLYD 
                   
               
               
                   
                   
                 taaagtaccagaagatgccactgcttacggtcataga 
                   
                 VLAKAVPESAGHAY 
                   
               
               
                   
                   
                 gatgttctattctgggttcaaatttttatgatcaatccatta 
                   
                 LGCPDPRMPNAQQA 
                   
               
               
                   
                   
                 ggtccagtttctcaaacgatctacgatttcgctgacggc 
                   
                 YWRNNLPRLEELKG 
                   
               
               
                   
                   
                 ttgtatgacgttctggctaaggccgtacctgaatccgct 
                   
                 DLDPKDIFHNPQGV 
                   
               
               
                   
                   
                 ggtcacgcatacctaggttgtcccgacccaagaatgc 
                   
                 MVVS 
                   
               
               
                   
                   
                 ctaacgctcaacaggcctactggaggaacaacttgcc 
                   
                   
                   
               
               
                   
                   
                 aagattggaagaattgaagggtgacttagatccaaaa 
                   
                   
                   
               
               
                   
                   
                 gatattttccataatcctcaaggagtgatggtcgtgagc 
                   
                   
                   
               
               
                   
               
               
                 t808237 
                 Library 
                 atgggtaatacgacttccatcgccggccgtgactgctt 
                 99 
                 MGNTTSIAGRDCLV 
                 169 
               
               
                   
                   
                 ggttagtgcactaggtggaaacgctggtttagtggcttt 
                   
                 SALGGNAGLVAFQD 
                   
               
               
                   
                   
                 ccaagatcagcttttgtatcaaaccacagctgtacacg 
                   
                 QLLYQTTAVHEYNL 
                   
               
               
                   
                   
                 agtacaacttgaacattccagtcacccctgccgcagtt 
                   
                 NIPVTPAAVTYPETA 
                   
               
               
                   
                   
                 acttacccagaaactgctgaacaaatagctgccgtcgt 
                   
                 EQIAAVVKCASEYD 
                   
               
               
                   
                   
                 gaaatgtgcttctgaatatgattacaaggtccaagctag 
                   
                 YKVQARSGGHSFGN 
                   
               
               
                   
                   
                 atctggtggacattcgtttggtaattacggtctaggtggt 
                   
                 YGLGGADGAVVVD 
                   
               
               
                   
                   
                 gctgacggtgctgtagttgttgatatgaagcacttctca 
                   
                 MKHFSQFSMDDQTY 
                   
               
               
                   
                   
                 caattttccatggacgatcagacatatgaagcagttatc 
                   
                 EAVIGPGTTLGDVD 
                   
               
               
                   
                   
                 ggtcccggtaccactttaggtgacgtcgacaccgaatt 
                   
                 TELYNNGKRAMAH 
                   
               
               
                   
                   
                 gtacaacaacggcaagagagctatggcccatggtatt 
                   
                 GICPTISTGGHFTMG 
                   
               
               
                   
                   
                 tgtccaacaattagtactggtggacacttcactatgggt 
                   
                 GLGPTARQWGLALD 
                   
               
               
                   
                   
                 ggtctgggtccaaccgccagacaatggggtttggcttt 
                   
                 HVEEVEVVLANSSIV 
                   
               
               
                   
                   
                 ggatcacgttgaagaggttgaagtcgttttggcaaattc 
                   
                 RASNTQNQEVFFAV 
                   
               
               
                   
                   
                 ttctatcgttagggcttccaacacccaaaatcaagaagt 
                   
                 KGAAASFGIVTEFK 
                   
               
               
                   
                   
                 cttctttgctgtcaaaggtgccgctgcctcatttggtatc 
                   
                 VRTQPAPGLAVQYS 
                   
               
               
                   
                   
                 gttacagagttcaaggtcagaactcaacctgctccag 
                   
                 YTFNIGSSAEKAQFV 
                   
               
               
                   
                   
                 gcttagcagtacagtacagctatacgtttaatattggttc 
                   
                 KDWQSFISAKNLTR 
                   
               
               
                   
                   
                 gtctgctgaaaaggcccaattcgttaaagattggcaat 
                   
                 QFYTNMVIFDGDIIL 
                   
               
               
                   
                   
                 cattcattagtgctaagaaccttactagacaattctacac 
                   
                 EGLFFGSQEQYEAL 
                   
               
               
                   
                   
                 caacatggtaatcttcgatggtgacataattttggaagg 
                   
                 GLEDRFVPKNPGNIL 
                   
               
               
                   
                   
                 attatttttcggttcccaagaacaatatgaagctttgggt 
                   
                 VLTDWLGMVGHAL 
                   
               
               
                   
                   
                 ctggaagacagatttgttccaaagaaccctggaaatat 
                   
                 EDTILRLVGNTPTWF 
                   
               
               
                   
                   
                 tttggtgttgacggattggctgggtatggttggtcatgc 
                   
                 YAKSLGFTPDTLIPA 
                   
               
               
                   
                   
                 ccttgaagacactatcttaagattggtcggtaacactcc 
                   
                 SGIDEFFDYIENHKA 
                   
               
               
                   
                   
                 aacttggttttacgctaaatctttgggattcaccccagac 
                   
                 GTLTWFVTLSLEGG 
                   
               
               
                   
                   
                 actttaattccagcttccggtatcgatgaatttttcgatta 
                   
                 AINDVPEDATAYGH 
                   
               
               
                   
                   
                 catagaaaaccataaggcaggcaccttgacgtggttc 
                   
                 RDVLFWVQIFMASP 
                   
               
               
                   
                   
                 gtcactttgtctctggaaggtggtgctatcaatgatgtc 
                   
                 TGPVSSTTYDFADG 
                   
               
               
                   
                   
                 ccagaggacgctacagcctacggtcatagagatgtttt 
                   
                 LYNVLTKAVPESEG 
                   
               
               
                   
                   
                 gttctgggttcaaatttttatggcttctcccaccggtcct 
                   
                 HAYLGCPDPKMAD 
                   
               
               
                   
                   
                 gtctcctctaccacctatgacttcgccgatggtctatata 
                   
                 AQQKYWRQNLPRL 
                   
               
               
                   
                   
                 atgttttaactaaggctgtaccagagagcgaaggtcac 
                   
                 EELKATLDPKDTFH 
                   
               
               
                   
                   
                 gcttacttaggttgtccagaccctaagatggccgatgc 
                   
                 NPQGILPA 
                   
               
               
                   
                   
                 tcagcaaaaatactggcgtcaaaacttgccaagattgg 
                   
                   
                   
               
               
                   
                   
                 aagaattgaaggcaactttagacccaaaagataccttc 
                   
                   
                   
               
               
                   
                   
                 cacaatccccaaggtatcttgccagct 
                   
                   
                   
               
               
                   
               
               
                 t808238 
                 Library 
                 atgtggttgtctacaatgaatggttcagccagtagacgt 
                 100 
                 MWLSTMNGSASRRS 
                 170 
               
               
                   
                   
                 agcgatcccgtcagcagaaaaatcgtttgcgacggcc 
                   
                 DPVSRKIVCDGHAS 
                   
               
               
                   
                   
                 atgcttctgcacacgaggtgaggactgacaacgaag 
                   
                 AHEVRTDNEAARDV 
                   
               
               
                   
                   
                 ctgctagagatgtaccttcgagaaccgctgtcaacaa 
                   
                 PSRTAVNKERKQGS 
                   
               
               
                   
                   
                 ggaaagaaagcagggttccggtccaccaggagccat 
                   
                 GPPGAMQRGFHAA 
                   
               
               
                   
                   
                 gcaaagaggttttcacgctgcccataagccaaatgaa 
                   
                 HKPNEMVPQDGPLG 
                   
               
               
                   
                   
                 atggttccacaagacggtcctcttggtagaactgctca 
                   
                 RTAQLFRLAPACQS 
                   
               
               
                   
                   
                 attattccgtctggcaccagcttgtcaatcagaaccaac 
                   
                 EPTRAPGQPSDLRLR 
                   
               
               
                   
                   
                 gagagctccaggtcaaccatctgatctaagattgcgtc 
                   
                 QIPLATEQAARTLAR 
                   
               
               
                   
                   
                 aaattcccttggcaaccgagcaagctgccagaactttg 
                   
                 MRPARFTFPYGRAA 
                   
               
               
                   
                   
                 gctaggatgcgtccagcaagattcacatttccttatgga 
                   
                 EDDCYLKKEDEGHD 
                   
               
               
                   
                   
                 agagccgctgaagatgattgttacttaaaaaaggaag 
                   
                 QSHPTSVLVGVPPFT 
                   
               
               
                   
                   
                 acgaaggtcacgaccagtctcatccaacctccgtcttg 
                   
                 RRCAAAETFKDTRA 
                   
               
               
                   
                   
                 gttggtgttccccctttcactagacgttgtgctgctgctg 
                   
                 RAPGTQPTDTTSTG 
                   
               
               
                   
                   
                 aaaccttcaaagatactagagctagagctccaggtac 
                   
                 ASPSWTLSPLLSLSA 
                   
               
               
                   
                   
                 acaaccaactgataccacttctacaggtgcctccccat 
                   
                 TDDSVPSKMGNGQS 
                   
               
               
                   
                   
                 catggaccttatctccactattgtccttgtctgctactga 
                   
                 TPLQQCLNTVCNGR 
                   
               
               
                   
                   
                 cgacagtgttccttccaagatgggcaacggtcaatcta 
                   
                 LGCVAFPSDALYQA 
                   
               
               
                   
                   
                 ccccacttcaacaatgtttgaatactgtttgcaacggta 
                   
                 AWVKPYNLDVPVTP 
                   
               
               
                   
                   
                 ggctgggttgtgtcgcctttccatcggatgccttatacc 
                   
                 IAVFKPSSTEDVAGA 
                   
               
               
                   
                   
                 aggctgcttgggtcaagccatataacttggacgtacct 
                   
                 IKCAVASNVHVQAK 
                   
               
               
                   
                   
                 gttaccccaatagctgtgtttaagcctagttccacggag 
                   
                 SGGHSYANFGLGGQ 
                   
               
               
                   
                   
                 gatgttgctggtgccattaagtgtgctgtcgcttctaac 
                   
                 DGELMIDLANLQDF 
                   
               
               
                   
                   
                 gttcacgtgcaagccaagtctggtggtcattcgtacgc 
                   
                 HMDKTSWQATFGA 
                   
               
               
                   
                   
                 taacttcggattgggtggacaagatggtgaactaatga 
                   
                 GYRLGDLDKKLQA 
                   
               
               
                   
                   
                 ttgatttggcaaatttacaggacttccacatggacaaaa 
                   
                 NGNRAIAHGTCPGV 
                   
               
               
                   
                   
                 catcctggcaagctactttcggtgccggttacagattg 
                   
                 GIGGHATIGGLGPMS 
                   
               
               
                   
                   
                 ggtgatttagataaaaagttacaagcaaatggcaaca 
                   
                 RMWGSALDHVLSV 
                   
               
               
                   
                   
                 gagctatcgcacacggcacatgtccaggtgttggtatt 
                   
                 QVVTADGSIKNASE 
                   
               
               
                   
                   
                 ggaggtcatgccactatcggcggtctaggcccaatga 
                   
                 SENSDLFWALRGAG 
                   
               
               
                   
                   
                 gccgtatgtggggttccgctttggaccacgtcttgtctg 
                   
                 ASFGVITKFTVKTHP 
                   
               
               
                   
                   
                 tccaagttgtcaccgctgacggtagtatcaaaaacgcc 
                   
                 APGSVVQYTYKISL 
                   
               
               
                   
                   
                 tctgaatcagaaaactctgatctgttttgggccttgaga 
                   
                 GSQAQMAPVYAAW 
                   
               
               
                   
                   
                 ggtgctggtgcttcatttggagttattactaagttcactgt 
                   
                 QALAGDPKLDRRFS 
                   
               
               
                   
                   
                 taagacccatccagctccaggttccgttgtacaatatac 
                   
                 TLFIAEPLGALITGTF 
                   
               
               
                   
                   
                 atacaaaatctctttgggtagccaggcacaaatggccc 
                   
                 YGTKAEYEATGIAA 
                   
               
               
                   
                   
                 ctgtttacgctgcctggcaagctttagctggtgacccc 
                   
                 RLPSGGTLDLKLLD 
                   
               
               
                   
                   
                 aagctggacagaagattttccacattgttcatcgcaga 
                   
                 WLGSLAHIAEVVGL 
                   
               
               
                   
                   
                 acctcttggtgctctgattaccggaactttctatggtact 
                   
                 TLGDIPTSFYGKSLA 
                   
               
               
                   
                   
                 aaagctgagtacgaagctactggtatagctgctagatt 
                   
                 LREEDMLDRTSIDGL 
                   
               
               
                   
                   
                 gccttctggtggtaccttggatttgaagcttttagattgg 
                   
                 FRYMGDADAGTLL 
                   
               
               
                   
                   
                 ttgggtagtttggctcacatagctgaagtagttggtttga 
                   
                 WFVIFNSEGGAMAD 
                   
               
               
                   
                   
                 ccttgggtgacattccaacgtccttttacggtaagtcgc 
                   
                 TPAGATAYPHRDKL 
                   
               
               
                   
                   
                 tagctttgagagaagaagacatgttagaccgtacttcta 
                   
                 IMYQSYVIGIPTLTK 
                   
               
               
                   
                   
                 ttgatggtttgttcaggtatatgggtgacgccgatgcag 
                   
                 ATRDFADGVHDRVR 
                   
               
               
                   
                   
                 gcaccctattatggttcgtcatcttcaattccgagggtg 
                   
                 MGAPSANSTYAGYI 
                   
               
               
                   
                   
                 gtgccatggctgatactccagctggtgccaccgccta 
                   
                 DRTLSREAAQEFYW 
                   
               
               
                   
                   
                 cccacatagagataaattaatcatgtatcaatcatacgtt 
                   
                 GAQLPRLREVKKA 
                   
               
               
                   
                   
                 attggtataccaaccctgactaaggccaccagagattt 
                   
                 WDPKDVFHNPQSVD 
                   
               
               
                   
                   
                 tgctgatggtgtccacgaccgtgtgagaatgggtgctc 
                   
                 PAE 
                   
               
               
                   
                   
                 catctgctaacagtacgtatgcaggttacatcgataga 
                   
                   
                   
               
               
                   
                   
                 accttgtccagagaagctgctcaagaattttactgggg 
                   
                   
                   
               
               
                   
                   
                 cgctcaactgcctagattgagagaagtcaagaaagca 
                   
                   
                   
               
               
                   
                   
                 tgggacccaaaggacgtctttcacaacccacaatccg 
                   
                   
                   
               
               
                   
                   
                 ttgatccagcagag 
                   
                   
                   
               
               
                   
               
               
                 t808240 
                 Library 
                 atgggcaatacaacttccattggtgtagtgagggattgt 
                 101 
                 MGNTTSIGVVRDCL 
                 171 
               
               
                   
                   
                 ttgacgtctgctgtcggtggtgttgcagcccatgtcgct 
                   
                 TSAVGGVAAHVAFQ 
                   
               
               
                   
                   
                 ttccaggacaccctattataccaaacctcagctgttaaa 
                   
                 DTLLYQTSAVKPYN 
                   
               
               
                   
                   
                 ccatataaccttaacgtccctgttactcccgccgctgtt 
                   
                 LNVPVTPAAVTYPQ 
                   
               
               
                   
                   
                 acttacccacaaagtgctaatgaagtcgctgctatcgtt 
                   
                 SANEVAAIVKCASD 
                   
               
               
                   
                   
                 aagtgcgcatcggattatgactacaaggtacaagctc 
                   
                 YDYKVQARSGGHSF 
                   
               
               
                   
                   
                 gttccggtggacacagctttggtaacttcggtttaggtg 
                   
                 GNFGLGGQNGAIVI 
                   
               
               
                   
                   
                 gacaaaacggtgccatagttattgacatgaaacactttt 
                   
                 DMKHFSQFSMDEST 
                   
               
               
                   
                   
                 ctcaattctccatggatgagtctaccttcatcgccactat 
                   
                 FIATIGPGTTLGNLD 
                   
               
               
                   
                   
                 tggcccaggcaccactttgggtaatctggatacagaat 
                   
                 TELYNAGNRAMAH 
                   
               
               
                   
                   
                 tgtacaacgctggtaatagagctatggctcatggtatat 
                   
                 GICPSIRTGGHLTVG 
                   
               
               
                   
                   
                 gtccatcgatcagaactggtggtcacttgaccgttgga 
                   
                 GLGPTSRQWGLALD 
                   
               
               
                   
                   
                 ggtttgggtcctacctctcgtcaatggggtctagctctg 
                   
                 HVEEVEVVLANSSV 
                   
               
               
                   
                   
                 gaccacgtcgaagaggtggaagttgtacttgctaactc 
                   
                 VRASDTQNQDVLFA 
                   
               
               
                   
                   
                 ttcagtcgtcagagcctctgacacgcagaaccaagat 
                   
                 IKGAAASFGIVTEFK 
                   
               
               
                   
                   
                 gttttatttgctatcaagggtgcagccgcatccttcggta 
                   
                 VRTEEAPGLAVRYS 
                   
               
               
                   
                   
                 tcgttactgaatttaaggtcagaacagaagaagctcca 
                   
                 YSFNLGTPAEKAKL 
                   
               
               
                   
                   
                 ggtttggccgttagatattcctacagcttcaacttgggta 
                   
                 AKDWQAYIAQENLT 
                   
               
               
                   
                   
                 ctccagctgaaaaagcaaagttggctaaggattggca 
                   
                 WKFSSNLIIFDGQIIL 
                   
               
               
                   
                   
                 agcctacattgcccaagaaaacttaacgtggaaattct 
                   
                 EGIFFGSKEEYDKLN 
                   
               
               
                   
                   
                 ctagtaacttgattattttcgacggtcaaattatccttgag 
                   
                 LEKKFPTSEPGTVLV 
                   
               
               
                   
                   
                 ggaatatttttcggtagcaaggaagaatacgacaagtt 
                   
                 ITNWLGMIGHALED 
                   
               
               
                   
                   
                 aaatttggaaaagaagtttccaacttcagaacctggta 
                   
                 TILRLIGDSPTWFYA 
                   
               
               
                   
                   
                 ccgtcttggtcattacgaattggttgggtatgatcggac 
                   
                 KSLGFTPNTLIFDSTI 
                   
               
               
                   
                   
                 atgctttggaagataccatcctaagacttatcggtgatt 
                   
                 DEFFDYIHKANAGT 
                   
               
               
                   
                   
                 cacccacttggttctatgctaaatctttgggttttactcca 
                   
                 LAWSVMLSLEGGAI 
                   
               
               
                   
                   
                 aacacactaatctttgactctaccattgacgaatttttcg 
                   
                 NAVPKNATAYGHR 
                   
               
               
                   
                   
                 attacatacacaaggctaacgctggtacattagcttggt 
                   
                 DVLFWVQIFVVNPL 
                   
               
               
                   
                   
                 ccgttatgttgtctttggaaggtggtgccataaatgctgt 
                   
                 GPISQTTYGFTDGLY 
                   
               
               
                   
                   
                 tccaaaaaatgctactgcatacggtcatagagatgtatt 
                   
                 NILARGVPESAGHA 
                   
               
               
                   
                   
                 attctgggttcaaattttcgttgtgaatcctcttggaccaa 
                   
                 YLGCPDPKMPDAQR 
                   
               
               
                   
                   
                 tttcccaaaccacttatggttttaccgatggtttgtataac 
                   
                 AYWRNNYPRLEELK 
                   
               
               
                   
                   
                 atcttggccagaggtgttccagagtccgcaggtcatg 
                   
                 RDLDPKDIFHNPQG 
                   
               
               
                   
                   
                 cttacttaggttgtccagatcccaagatgccagacgct 
                   
                 VRVAS 
                   
               
               
                   
                   
                 caaagagcatactggagaaataactatccacgtctgg 
                   
                   
                   
               
               
                   
                   
                 aggaattgaaaagagacttggatcctaaggacatttttc 
                   
                   
                   
               
               
                   
                   
                 acaacccacagggcgtcagagtcgcttct 
                   
                   
                   
               
               
                   
               
               
                 t808247 
                 Library 
                 atgggcaacactacatcaattgctgccggtagagattg 
                 102 
                 MGNTTSIAAGRDCL 
                 172 
               
               
                   
                   
                 cctagtaagcgcagtcggtccagctcatgttaccttcc 
                   
                 VSAVGPAHVTFQDA 
                   
               
               
                   
                   
                 aggacgcccttctgtaccaaactacggctgtcgatcct 
                   
                 LLYQTTAVDPYNLN 
                   
               
               
                   
                   
                 tataatttaaacatcccagtgacccccgctgctgttactt 
                   
                 IPVTPAAVTYPQSAE 
                   
               
               
                   
                   
                 acccacaatcggctgaagagatagccgctgttgtcaa 
                   
                 EIAAVVKCASDYDY 
                   
               
               
                   
                   
                 atgtgcttctgactatgattacaaggttcaagctaggtct 
                   
                 KVQARSGGHSFGNY 
                   
               
               
                   
                   
                 ggtggacactcctttggtaactacggtttgggtggtca 
                   
                 GLGGQNGAIVVDM 
                   
               
               
                   
                   
                 aaatggagccattgtagttgacatgaagcacttctctca 
                   
                 KHFSQFSMDESTFV 
                   
               
               
                   
                   
                 atttagtatggatgaatctaccttcgtcgcaactattggt 
                   
                 ATIGPGTTLGDLDTE 
                   
               
               
                   
                   
                 ccaggtacaaccttgggcgacttggatactgaattgta 
                   
                 LYNAGGRAMAHGIC 
                   
               
               
                   
                   
                 taacgcaggcggtagagctatggcccatggtatctgt 
                   
                 PTIRTGGHLTVGGLG 
                   
               
               
                   
                   
                 cctacaatccgtactggtggtcacttaactgtcggtggt 
                   
                 PTARQWGLALDHIE 
                   
               
               
                   
                   
                 ttgggtccaaccgctagacaatggggtctggccttag 
                   
                 EVEVVLANSSIVRAS 
                   
               
               
                   
                   
                 atcacattgaagaagttgaagtggttttggctaattcctc 
                   
                 NTQNQDILFAVKGA 
                   
               
               
                   
                   
                 gatagtgagagctagcaacactcagaaccaagacat 
                   
                 AASFGIVTEFKVRTQ 
                   
               
               
                   
                   
                 cttgttcgccgttaagggtgctgctgcttcatttggtatt 
                   
                 EAPGLAVQYSFTFN 
                   
               
               
                   
                   
                 gtcaccgagtttaaagttagaacccaagaagcaccag 
                   
                 LGSPAQKAKLVKD 
                   
               
               
                   
                   
                 gactagctgttcaatacagtttcaccttcaatttgggttc 
                   
                 WQAFIAQENLSWKF 
                   
               
               
                   
                   
                 cccagctcagaaagccaagttggtcaaggactggca 
                   
                 YSNLVIFDGQIILEGI 
                   
               
               
                   
                   
                 agcattcattgcccaagaaaacttatcttggaagttcta 
                   
                 FFGSKEEYDELDLEK 
                   
               
               
                   
                   
                 ctctaatttagtcatctttgacggtcaaattattttagaag 
                   
                 RFPTSEPGTVLVLTD 
                   
               
               
                   
                   
                 gtatctttttcggatccaaggaggaatatgatgaattgg 
                   
                 WLGMIGHALEDTIL 
                   
               
               
                   
                   
                 acttggaaaaaagatttcccacttctgaaccaggtaca 
                   
                 KLVGDTPTWFYAKS 
                   
               
               
                   
                   
                 gttctggttttaacggattggttgggaatgatcggccat 
                   
                 LGFTPDTLIPDSAIDD 
                   
               
               
                   
                   
                 gcacttgaggatactattttgaagttggtcggtgacaca 
                   
                 FFDYIHKTNAGTLA 
                   
               
               
                   
                   
                 cctacgtggttttacgctaagtcccttggcttcactcca 
                   
                 WFVTLSLEGGAINS 
                   
               
               
                   
                   
                 gataccttgatcccagattcggctattgatgatttcttcg 
                   
                 VSEDATAYGHRDVL 
                   
               
               
                   
                   
                 actatattcataagactaacgctggtactctggcctggt 
                   
                 FWFQVFVVNPLGPIS 
                   
               
               
                   
                   
                 ttgtgaccttatctttggaaggtggcgctataaactccgt 
                   
                 QTTYDFTNGLYDVL 
                   
               
               
                   
                   
                 ttcagaagatgctaccgcttatggtcacagagatgtctt 
                   
                 AQAVPESAGHAYLG 
                   
               
               
                   
                   
                 gttttggttccaagttttcgttgtcaatcctcttggtccaat 
                   
                 CPDPKMPDAQRAY 
                   
               
               
                   
                   
                 ctctcaaacaacatacgacttcactaatggtttgtacga 
                   
                 WRSNLPRLEDLKGD 
                   
               
               
                   
                   
                 cgtattggctcaggccgtgcctgaaagcgctggtcat 
                   
                 LDPKDTFHNPQGVQ 
                   
               
               
                   
                   
                 gcttaccttggttgtccagatccaaaaatgccagacgc 
                   
                 VGP 
                   
               
               
                   
                   
                 tcagcgtgcttactggagaagtaacttacccagattgg 
                   
                   
                   
               
               
                   
                   
                 aggatctgaagggtgatcttgacccaaaggacaccttt 
                   
                   
                   
               
               
                   
                   
                 cacaaccctcaaggtgttcaagtcggtcca 
                   
                   
                   
               
               
                   
               
               
                 t808253 
                 Library 
                 atgggcaataccacatctatcgctgccggtagagact 
                 103 
                 MGNTTSIAAGRDCL 
                 173 
               
               
                   
                   
                 gtctggtcagtgctgttggtcctgcacacgtgacgtttc 
                   
                 VSAVGPAHVTFQDA 
                   
               
               
                   
                   
                 aggatgctttgctttaccaaactactgctgttgatcccta 
                   
                 LLYQTTAVDPYNLN 
                   
               
               
                   
                   
                 taacttaaacataccagtaaccccagccgctgtcactt 
                   
                 IPVTPAAVTYPQSAE 
                   
               
               
                   
                   
                 acccacaatccgctgaggaaattgccgctgttgtgaa 
                   
                 EIAAVVKCASDYDY 
                   
               
               
                   
                   
                 gtgcgcttcagactacgattataaagtccaagctaggt 
                   
                 KVQARSGGHSFGNY 
                   
               
               
                   
                   
                 ctggaggtcatagcttcggtaactacggtctaggtggt 
                   
                 GLGGQNGAIVVEMK 
                   
               
               
                   
                   
                 caaaatggtgcaatcgttgttgaaatgaagcacttctct 
                   
                 HFSQFSMDESTFVAT 
                   
               
               
                   
                   
                 caattttccatggacgaatcgaccttcgtcgccactatt 
                   
                 IGPGTTLGDLDTELY 
                   
               
               
                   
                   
                 ggcccaggtacaacattgggtgatttagataccgaatt 
                   
                 NTGGRAMAHGICPT 
                   
               
               
                   
                   
                 gtataatactggtggccgtgctatggcccatggtatttg 
                   
                 IRTGGHLTVGGLGPT 
                   
               
               
                   
                   
                 tccaactatcagaaccggtggtcacttgaccgttggtg 
                   
                 ARQWGLALDHIEEV 
                   
               
               
                   
                   
                 gattgggtcctactgcaagacaatggggtttagctcttg 
                   
                 EVVLANSSIVRASNT 
                   
               
               
                   
                   
                 atcatatcgaagaagttgaggtcgtcttggctaactctt 
                   
                 QNQDILFAVKGAAA 
                   
               
               
                   
                   
                 ccattgttagagctagcaacactcagaaccaagacatt 
                   
                 SFGIVTEFKVRTQEA 
                   
               
               
                   
                   
                 ctatttgctgttaaaggagccgctgccagcttcggtata 
                   
                 PGLAVQYSFTFNLGS 
                   
               
               
                   
                   
                 gtcaccgaatttaaggttagaacacaggaagctccag 
                   
                 AAQKAKLVKDWQA 
                   
               
               
                   
                   
                 gtttggctgtacaatacagtttcaccttcaatttgggctc 
                   
                 FIAQENLSWKFYSNL 
                   
               
               
                   
                   
                 agcagctcaaaaggcaaagttggtcaaagactggca 
                   
                 VIFDGQIILEGIFFGS 
                   
               
               
                   
                   
                 agccttcatcgctcaagaaaatttatcttggaaattttact 
                   
                 KEEYDELDLEKRFPT 
                   
               
               
                   
                   
                 ctaacctagttatttttgacggacaaattatcttggaagg 
                   
                 SEPGTVLVLTDWLG 
                   
               
               
                   
                   
                 tatcttcttcggttccaaggaggaatacgatgaactaga 
                   
                 MIGHGLEDTILKLVG 
                   
               
               
                   
                   
                 cttagaaaagagattcccaacttctgaaccaggtaccg 
                   
                 DTPTWFYAKSLGFT 
                   
               
               
                   
                   
                 tgttggttttaactgattggttgggtatgatcggtcacgg 
                   
                 PDTLIPDSAIDDFFD 
                   
               
               
                   
                   
                 tctggaagacactatattgaagttagttggtgatacccc 
                   
                 YIHKTNAGTLAWFV 
                   
               
               
                   
                   
                 tacttggttctatgcaaagtccttgggttttacgccagat 
                   
                 TLSLEGGAINSVSED 
                   
               
               
                   
                   
                 actttgatacccgattctgccattgacgattttttcgattat 
                   
                 ATAYGHRDVLFWF 
                   
               
               
                   
                   
                 attcataagacaaatgctggaaccttggcttggtttgta 
                   
                 QVFVVNPLGPISQTT 
                   
               
               
                   
                   
                 acgctatctttggaaggtggtgctataaactctgtctcg 
                   
                 YDFTNGLYDVLAQA 
                   
               
               
                   
                   
                 gaagacgcaacagcttacggtcacagagatgtcctgt 
                   
                 VPESAGHAYLGCPD 
                   
               
               
                   
                   
                 tttggttccaagtgtttgtagtcaaccctttgggtccaatt 
                   
                 PKMPDAQRAYWRS 
                   
               
               
                   
                   
                 tcccagaccacttacgacttcaccaatggtttatacgat 
                   
                 NLPRLEDLKGDLDP 
                   
               
               
                   
                   
                 gttcttgctcaagccgttccagaatcggccggccacg 
                   
                 KDTFHNPQGVQVGP 
                   
               
               
                   
                   
                 cttatttgggttgtccagaccctaaaatgcccgacgca 
                   
                   
                   
               
               
                   
                   
                 caacgtgcttactggaggtccaacctaccaagattgg 
                   
                   
                   
               
               
                   
                   
                 aggacttaaagggtgacctagacccaaaggatactttt 
                   
                   
                   
               
               
                   
                   
                 cataacccacaaggtgtccaagttggacca 
               
               
                   
               
            
           
         
       
     
     It should be appreciated that sequences disclosed in this application may or may not contain signal sequences. The sequences disclosed in this application encompass versions with or without signal sequences. It should also be understood that protein sequences disclosed in this application may be depicted with or without a start codon (M). The sequences disclosed in this application encompass versions with or without start codons. Accordingly, in some instances amino acid numbering may correspond to protein sequences containing a start codon, while in other instances, amino acid numbering may correspond to protein sequences that do not contain a start codon. It should also be understood that sequences disclosed in this application may be depicted with or without a stop codon. The sequences disclosed in this application encompass versions with or without stop codons. Aspects of the disclosure encompass host cells comprising any of the sequences described in this application and fragments thereof. 
     EQUIVALENTS 
     Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described here. Such equivalents are intended to be encompassed by the following claims. 
     All references, including patent documents, are incorporated by reference in their entirety.