Patent Publication Number: US-2023159989-A1

Title: Multiplexed fluorescence in situ hybridization method capable of rapid detection of billions of targets

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application claims the benefit of U.S. Provisional Application No. 63/282,947, filed on Nov. 24, 2021, and U.S. Provisional Application No. 63/339,291, filed on May 6, 2022. The entire contents of the aforementioned applications are incorporated herein by reference in their entireties. 
    
    
     SEQUENCE LISTING 
     This application includes and incorporates by reference in its entirety a Sequence Listing XML in the required .xml format. The Sequence Listing XML file that has been electronically filed contains the information of the nucleotide and/or amino acid sequences disclosed in the patent application using the symbols and format in accordance with the requirements of 37 C.F.R. §§ 1.832 through 1.834. 
     The Sequence Listing XML filed herewith serves as the electronic copy required by § 1.834(b)(1). 
     The Sequence Listing XML is identified as follows: “KANVAS_003_SEQ_LIST.xml” (1649 kilo bytes in size), which was created on Nov. 22, 2022. 
     TECHNICAL FIELD 
     This disclosure relates to methods for highly-multiplexed, rapid detection of nucleotides in samples, and constructs to be used in said methods. 
     BACKGROUND 
     Microbes, both individually and in communities (i.e. microbiomes), play a large role in human health and disease. Conventional methods to study biologically and clinically relevant aspects of these microbes, including antimicrobial resistance, suffer from long turnaround times and are limited in the number of taxa and genetic elements they can profile. As a result, researchers are left with an incomplete understanding of microbiota in their native biological contexts. In addition, clinicians are faced with diagnostic delays that are detrimental to patient care, which increases the risk of patient morbidity and mortality. 
     SUMMARY 
     Antimicrobial resistance is an emerging threat to global public health. Current tests available in clinical laboratories are time-consuming and limited in scope for antimicrobial resistance profile measurement. Timely and accurate information on pathogen identity and their associated antimicrobial susceptibility profile is critical in helping clinicians treat patients with shorter response time and higher precision. In addition, many other microbial phenotypes, such as persistence, tolerance, motility, hyphae formation, spore formation, and quorum sensing, can provide useful biological and clinical information, but are difficult to measure using standard sequencing techniques. The present disclosure provides methods for microbial identification and rapid antimicrobial susceptibility profile measurement or other microbial phenotype measurements. These methods combine a short period of culturing with known concentrations of antimicrobial drugs, or other alterations to the environment, with a highly multiplexed fluorescence readout to distinguish cellular taxonomic identity and susceptibility to different classes of antimicrobials or other relevant microbial phenotypes. This approach will enable a rapid and cost-effective test that can be deployed in clinical settings for fast diagnosis of infectious agents and proper selection of antimicrobial drugs for treatment. 
     The present disclosure provides methods that combine single-cell imaging, single-molecule imaging, microfluidic technologies, and phenotypic antimicrobial susceptibility testing to enable rapid identification of microbial species, current antimicrobial susceptibility profile, and future antimicrobial susceptibility profile, directly from patient samples. The present disclosure also provides methods that enable the detection of millions or billions of potential nucleic acid based targets in a single assay. 
     The present disclosure provides methods that can rapidly identify microbial species, genera, families, orders, classes, and phyla associated with a particular tissue or specimen. In further embodiments, the present disclosure provides methods to rapidly determine any antimicrobial drugs or compounds the identified microbial species is susceptible to or to which the microbial species may become susceptible in the future. 
     In some aspects, the present disclosure provides methods of characterizing a microbial cell from a biological sample, the method comprising a) directly inoculating the microbe onto a device; b) identifying the microbe; and c) detecting susceptibility to one or more antimicrobial agents. 
     In some aspects, the present disclosure provides methods of characterizing a microbial cell from a biological sample, the method comprising a) directly inoculating the microbe onto a device; b) identifying the microbe; and c) detecting future susceptibility to one or more antimicrobial agents. 
     In some embodiments, the sample is not subjected to culturing before the microbe is inoculated onto the device. In some embodiments, the microbe in the sample is cultured for one to 12 cell divisions before it is inoculated onto the device. In some embodiments, the microbe in the sample is cultured for one to numerous cell divisions before it is inoculated onto the device. The number of cell divisions depends on the species doubling time, which can be variable. 
     In some embodiments, the microbe is identified by in situ hybridization. In some embodiments, the microbe is identified by fluorescence in situ hybridization (FISH). In some embodiments, the fluorescence in situ hybridization is high-phylogenetic-resolution fluorescence in situ hybridization (HiPR-FISH). 
     In some embodiments, the microbe is further characterized via live-cell imaging or growth dynamics calculation while in situ hybridization is performed. 
     In some embodiments, the microbe is identified by hybridization of a bar-coded probe a 16S ribosomal RNA sequence in the microbe, 5S ribosomal RNA sequence in the microbe, and/or 23S ribosomal RNA sequence in the microbe. In some embodiments, the in situ hybridization is multiplexed. In some embodiments, the susceptibility to one or more microbial agents is determined by measuring the minimum inhibitory concentration of the microbe when exposed to an antimicrobial agent. In some embodiments, the susceptibility to one or more microbial agents is determined by measuring microbial cell metabolism when the microbe is exposed to an antimicrobial agent. In some embodiments, microbial cell metabolism is measured by determining the concentration of dissolved carbon dioxide, oxygen consumption of microbes in the sample, expression of genes involved in cell division and/or growth, or expression of stress response genes. In some embodiments, microbial cell susceptibility is determined by a live/dead stain. In some embodiments, wherein microbial cell susceptibility is determined by cell number. In some embodiments, microbial cell susceptibility is determined by detecting the presence or absence of one or more antimicrobial genes in the microbial cell. In some embodiments, microbial cell susceptibility is determined by detecting the presence or absence of one or more gene mutations associated with the development of antimicrobial resistance or susceptibility in the microbial cell. In some embodiments, future microbial cell susceptibility is determined by detecting the presence or absence of one or more antimicrobial genes in the microbial cell. In some embodiments, future microbial cell susceptibility is determined by detecting the presence or absence of one or more gene mutations associated with the development of antimicrobial resistance or susceptibility in the microbial cell. 
     In some embodiments, wherein the one or more gene mutations associated with the development of antimicrobial resistance or susceptibility is selected from deletions, duplications, single nucleotide polymorphisms (SNPs), frame-shift mutations, inversions, insertions, and/or nucleotide substitutions. In some embodiments, the one or more antimicrobial genes is selected from: genes encoding multidrug resistance proteins (e.g. PDR1, PDR3, PDR7, PDR9), ABC transporters (e.g. SNQ2, STE6, PDR5, PDR10, PDR11, YOR1), membrane associated transporters (GAS1, D4405), soluble proteins (e.g. G3PD), RNA polymerase, rpoB, gyrA, gyrB, 16S RNA, 23S rRNA, NADPH nitroreductase, sul2, strAB, tetAR, aac3-iid, aph, sph, cmy-2, floR, tetB; aadA, aac3-VIa, and sul1. In some embodiments, the presence or absence of one or more antimicrobial genes, or the gene mutation associated with the development of antimicrobial resistance or susceptibility in the microbial cell is detected using in situ hybridization. In some embodiments, the presence or absence of one or more antimicrobial genes, or the gene mutation associated with the development of antimicrobial resistance or susceptibility in the microbial cell is detected using fluorescence in situ hybridization (FISH). In some embodiments, the fluorescence in situ hybridization is high-phylogenetic-resolution fluorescence in situ hybridization (HiPR-FISH). 
     In some embodiments, the identification of the microbial cell and the detection of susceptibility or future susceptibility to one or more antimicrobial agents occurs sequentially. 
     In some embodiments, the identification of the microbial cell and the detection of susceptibility or future susceptibility to one or more antimicrobial agents occurs simultaneously. 
     In some embodiments, the identification of the microbial cell and the detection of susceptibility or future susceptibility to one or more antimicrobial agents occurs in parallel. 
     In some embodiments, the biological sample is obtained from a patient. In some embodiments, the biological sample is obtained from a patient diagnosed with or believed to be suffering from an infection or disorder. In some embodiments, the disease or disorder is an infection. In some embodiments, the infection is a bacterial, viral, fungal, or parasitic infections. In some embodiments, the bacterial infection is selected from  Mycobacterium, Streptococcus, Staphylococcus, Shigella, Campylobacter, Salmonella, Clostridium, Corynebacterium, Pseudomonas, Neisseria, Listeria, Vibrio, Bordetella, E. coli  (including pathogenic  E. coli ),  Pseudomonas aeruginosa, Enterobacter cloacae, Mycobacterium tuberculosis, Staphylococcus aureus, Helicobacter pylori, Legionella, Acinetobacter baumannii, Citrobacter freundii, Citrobacter koseri, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus saprophyticus , and  Streptococcus agalactiae , or a combination thereof. In some embodiments, the viral infection is selected from  Helicobacter pylori , infectious haematopoietic necrosis virus (IHNV), Parvovirus B19, Herpes Simplex Virus, Varicella-zoster virus, Cytomegalovirus, Epstein-Barr virus, Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Hepatitis D virus, Hepatitis E virus, Measles virus, Mumps virus, Rubella virus, Human Immunodeficiency Virus (HIV), Influenza virus, Rhinovirus, Rotavirus A, Rotavirus B, Rotavirus C, Respiratory Syncytial Virus (RSV), Varicella zoster, Poliovirus, Norovirus, Zika Virus, Dengue Virus, Rabies Virus, Newcastle Disease Virus, and White Spot Syndrome Virus, or a combination thereof. In some embodiments, the fungal infection is selected from  Aspergillus, Candida, Pneumocystis, Blastomyces, Coccidioides, Cryptococcus , and  Histoplasma , or a combination thereof. In some embodiments, the parasitic infection is selected from  Plasmodium  (i.e.  P. falciparum, P. malariae, P. ovale, P. knowlesi , and  P. vivax ),  Trypanosoma, Toxoplasma, Giardia , and  Leishmania, Cryptosporidium , helminthic parasites:  Trichuris  spp. (whipworms),  Enterobius  spp. (pinworms),  Ascaris  spp. (roundworms),  Ancylostoma  spp. and  Necator  spp. (hookworms),  Strongyloides  spp. (threadworms),  Dracunculus  spp. (Guinea worms),  Onchocerca  spp. and  Wuchereria  spp. (filarial worms),  Taenia  spp.,  Echinococcus  spp., and  Diphyllobothrium  spp. (human and animal cestodes),  Fasciola  spp. (liver flukes) and  Schistosoma  spp. (blood flukes), or a combination thereof. 
     In some embodiments, the biological sample is selected from bronchoalveolar lavage fluid (BAL), blood, serum, plasma, urine, cerebrospinal fluid, pleural fluid, synovial fluid, ocular fluid, peritoneal fluid, amniotic fluid, gastric fluid, lymph fluid, interstitial fluid, tissue homogenate, cell extracts, saliva, sputum, stool, physiological secretions, tears, mucus, sweat, milk, semen, seminal fluid, vaginal secretions, fluid from ulcers and other surface eruptions, blisters, and abscesses, and extracts of tissues including biopsies of normal, malignant, and suspect tissues or any other constituents of the body which may contain the microorganism of interest. In some embodiments, the biological sample is a human oral microbiome sample. In some embodiments, the biological sample is a whole organism. 
     In another aspect, a method for analyzing a sample can include:
         contacting at least one encoding probe with the sample to produce a first complex, wherein each encoding probe comprises a targeting sequence, a first landing pad sequence, and a second landing pad sequence;   adding at least one first emissive readout probe to the first complex, wherein the first emissive readout probe comprises a label and a sequence complementary to the first landing pad sequence;   acquiring one or more emission spectra from the first emissive readout probe;   adding an exchange probe to the sample, wherein the exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequence,   hybridizing the exchange probe to the first emissive readout probe to form a second complex;   removing the second complex from the sample,   adding at least one second emissive readout probe to the first complex, wherein the second emissive readout probe comprises a label and a sequence complementary to the second landing pad sequence;   acquiring one or more emission spectra from the second emissive readout probe;   repeating the aforementioned steps for at least one different encoding probe;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a species of interest; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     In certain embodiments, the first emissive readout probe sequence can be the same length as the first landing pad sequence. 
     In certain embodiments, the first emissive readout probe sequence can be at least 2 nucleotides longer than the first landing pad sequence. 
     In certain embodiments, the second emissive readout probe sequence can be the same length as the second landing pad sequence. 
     In certain embodiments, the second emissive readout probe sequence can be at least 2 nucleotides longer than the second landing pad sequence. 
     In another aspect, a method for analyzing a sample can include:
         generating a set of probes, wherein each probe comprises:   (i) a targeting sequence;   (ii) a first landing pad sequence; and   (iii) a second landing pad sequence;   contacting the set of probes with the sample to permit hybridization of the probes to nucleotides present in the sample to produce a complex;   adding a first set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   acquiring one or more emission spectra from the first emissive readout probe;   adding a set of exchange probes to the sample, wherein each exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequences,   hybridizing the exchange probes to the first emissive readout probes to form a second complex;   removing the second complex from the sample, adding a second set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   acquiring one or more emission spectra from the second emissive readout probe;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a species of interest; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     In certain embodiments, the emissive readout probe sequence can be the same length as the first or second landing pad sequence. 
     In certain embodiments, the emissive readout probe sequence can be at least 2 nucleotides longer than the first or second landing pad sequence. 
     In another aspect, a construct can include:
         a targeting sequence that is a region of interest on a nucleotide;   a first landing pad sequence;   a second landing pad sequence, wherein the second landing pad sequence is different from the first landing pad sequence;   a first emissive readout probe comprising a first label and a sequence complimentary to the first landing pad sequence;   an exchange probe comprising a 100% complementary sequence to the first emissive readout probe sequences; and   a second emissive readout probe comprising a second label and a sequence complimentary to the second landing pad sequence.       

     In certain embodiments, the first emissive readout probe sequence can be the same length as the first landing pad sequence. 
     In certain embodiments, the first emissive readout probe sequence can be at least 2 nucleotides longer than the first landing pad sequence. 
     In certain embodiments, the second emissive readout probe sequence can be the same length as the second landing pad sequence. 
     In certain embodiments, the second emissive readout probe sequence can be at least 2 nucleotides longer than the second landing pad sequence. 
     In another aspect, a library of constructs comprising a plurality of barcoded probes, wherein each barcoded probe comprises:
         a targeting sequence that is a region of interest on a nucleotide;   a first landing pad sequence;   a second landing pad sequence, wherein the second landing pad sequence is different from the first landing pad sequence;   a first emissive readout probe comprising a first label and a sequence complimentary to the first landing pad sequence;   an exchange probe comprising a 100% complementary sequence to the first emissive readout probe sequences; and   a second emissive readout probe comprising a second label and a sequence complimentary to the second landing pad sequence.       

     In certain embodiments, the first emissive readout probe sequence can be the same length as the first landing pad sequence. 
     In certain embodiments, the first emissive readout probe sequence can be at least 2 nucleotides longer than the first landing pad sequence. 
     In certain embodiments, the second emissive readout probe sequence can be the same length as the second landing pad sequence. 
     In certain embodiments, the second emissive readout probe sequence can be at least 2 nucleotides longer than the second landing pad sequence. 
     In another aspect, a method for analyzing a bacterial sample can include:
         contacting at least one encoding probe with the sample to produce a first complex, wherein each encoding probe comprises a targeting sequence, a first landing pad sequence, and a second landing pad sequence;   adding at least one first emissive readout probe to the first complex, wherein the first emissive readout probe comprises a label and a sequence complementary to the first landing pad sequence;   detecting the first emissive readout probe with a confocal microscope;   adding an exchange probe to the sample, wherein the exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequence,   hybridizing the exchange probe to the first emissive readout probe to form a second complex;   removing the second complex from the sample,   adding at least one second emissive readout probe to the first complex, wherein the second emissive readout probe comprises a label and a sequence complementary to the second landing pad sequence;   detecting the second emissive readout probe with a confocal microscope;   repeating the aforementioned steps for at least one different encoding probe;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a bacterium; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     In certain embodiments, the first emissive readout probe sequence can be the same length as the first landing pad sequence. 
     In certain embodiments, the first emissive readout probe sequence can be at least 2 nucleotides longer than the first landing pad sequence. 
     In certain embodiments, the second emissive readout probe sequence can be the same length as the second landing pad sequence. 
     In certain embodiments, the second emissive readout probe sequence can be at least 2 nucleotides longer than the second landing pad sequence. 
     In another aspect, a method for analyzing a bacterial sample can include:
         generating a set of probes, wherein each probe comprises:   (i) a targeting sequence;   (ii) a first landing pad sequence; and   (iii) a second landing pad sequence;   contacting the set of probes with the sample to permit hybridization of the probes to nucleotides present in the sample to produce a complex;   adding a first set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   detecting the first set of emissive readout probes in the sample with a confocal microscope;   adding a set of exchange probes to the sample, wherein each exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequences,   hybridizing the exchange probes to the first emissive readout probes to form a second complex;   removing the second complex from the sample,   adding a second set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   detecting the second set of emissive readout probes in the sample with a confocal microscope;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a bacterium; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     In certain embodiments, the emissive readout probe sequence can be the same length as the first or second landing pad sequence. 
     In certain embodiments, the emissive readout probe sequence can be at least 2 nucleotides longer than the first or second landing pad sequence. 
     Other aspects, embodiments, and features will be apparent from the following description, the drawings, and the claims. 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIGS.  1 A- 1 B  shows an exemplary method of rapid phenotypic profiling of antibiotic resistance followed by microbial identification using imaging.  FIG.  1 A  shows an experimental set-up.  FIG.  1 B  shows an example of a binary barcoding and spectral imaging approach for highly multiplexed labeling of microbes for taxonomic identification. 
         FIG.  2    shows an experimental work-flow for HiPR-FISH to identify a microbe in a sample and characterize a drug-resistance phenotype. 
         FIG.  3    shows  E. coli  detected in urine samples obtained from three different patients. HiPR-FISH was performed directly on three patient samples, each with over 100,000 CFU/mL of  E. coli . The images were collected from the first emission channel after excitation of a 561 nm laser (in agreement with dye corresponding to the readout probe used, Alexa546). 
         FIG.  4    shows a HiPR-FISH panel identifying species including  A. baumannii, C. freundii, S. saprophyticus , and a mixture of  A. baumannii  and  C. freundii . Maximum merged emission images from different laser excitation wavelengths (first three columns). The fourth column is a false-colored, merged image of 405 nm (blue), 488 nm (green), and 561 nm (red). The fifth column is a close-up of the white boxes in column 4. 
         FIG.  5    shows the ability of HiPR-FISH to report drug susceptibility and minimum inhibitory concentration (MIC), and determine antimicrobial resistance or susceptibility. A comparison of the first and last time points, after several hours growth on a HiPR-FISH chip, for several concentrations of meropenem for carbapenem-resistant and carbapenem-susceptible  K. pneumonia . The carbapenem-resistant  K. pneumoniae  grows beyond 2 μg/mL (* denotes MIC) in agreement with Clinical and Laboratory Standards Institute criteria. 
         FIG.  6    shows the ability of HiPR-FISH to detect fastidious and slow growing organisms in a synthetic mixture of fixed and digested  Candida  species. HiPR-FISH probes were designed to detect  C. tropicalis  (blue),  C. glabrata  (orange), and  C. albicans  (green) (colors not shown). 
         FIGS.  7 A- 7 C  shows gene expression measurements enable rapid detection of stress response in HiPR-FISH compatible manner.  FIG.  7 A  shows a schematic of an ultrarapid gene expression measurement assay that can be performed in 2 hours with only 5 minutes of exposure to stress. The results of the 2 hour assay, with  E. coli  rRNA and heat-shock response gene clpB mRNA are shown in  E. coli  grown at 30° C. ( FIG.  7 B ) and shocked at 46° C. ( FIG.  7 C ) for 5 minutes. Scale bars=20 μm. 
         FIG.  8    shows a schematic of HiPR-Swap. 
         FIG.  9    shows probe stripping and signal recovery in HiPR-Swap. Fixed monomicrobial stock of  E. coli, Klebsiella pneumoniae , and  Pseudomonas aeruginosa , were hybridized with species-specific encoding probes and individual readout probes in a single step (left column of images). Exchange buffer, with exchange probes for each readout, was added and incubated overnight to remove the readout probes (middle column). Signals for each species were recovered by adding back readout probes without encoding probes (right column). 
         FIG.  10    shows speed of stripping readout probes in HiPR-Swap. HiPR-Swap samples of  E. coli, Klebsiella pneumoniae , and  Pseudomonas aeruginosa , from  FIG.  9    were imaged after 5 days (column “After 5 days”). Exchange buffer, with exchange probes for each readout, was added and incubated for 1 hour to remove the readout probes (column “Strip—1 hr”). 
         FIG.  11    shows samples of  E. coli, Klebsiella pneumoniae , and  Pseudomonas aeruginosa , from  FIG.  10    were imaged after stripping overnight (column “Strip—overnight”). Signals for each species were recovered by adding different readout probes of green color without encoding probes (column “Swap—R #-488”). 
         FIG.  12    shows probe stripping and swapping reaction in a single step. Top panel shows single step strip and swap reaction and bottom panel shows sequential strip and swap reaction. Fixed synthetic mixtures of  E. coli  and  Pseudomonas aeruginosa  ( P. aeru; P. aeruginosa ) were hybridized with species-specific encoding probes and  Eubacterium  probes (conjugated with Rhodamine Red-X fluorophore). First row of each panel shows the  Eubacterium  signal. In round 1, only  E. coli  is hybridized with its readout probes for both the single step and sequential step conditions (left column second row of each panel). In round 2 of the single step condition, exchange buffer containing exchange probes for  E. coli  and readout probes for  P. aeruginosa  was added and incubated for 2 hours (top panel middle column second row). In round 2 of the sequential step condition, exchange buffer containing exchange probes for  E. coli  was added and incubated for 2 hours (bottom panel middle column second row). In round 3 of the single step condition, exchange buffer containing readout probes for  E. coli  and exchange probes for  P. aeruginosa  was added and incubated for 2 hours (top panel last column second row). In round 3 of sequential step condition, exchange buffer containing readout probes for  P. aeruginosa  was added and incubated for 2 hours (bottom panel last column second row). 
         FIG.  13    shows real time measurement of single step strip and swap reaction of HiPR-Swap. Fixed synthetic mixtures of  E. coli  and  Pseudomonas aeruginosa  ( P. aeru, P. aeruginosa ) were hybridized with species-specific encoding probes. Before performing single step stripping and swapping reaction, only  E. coli  is hybridized with its readout probes (Image: “Before”). While keeping the sample under the microscope, exchange buffer containing exchange probes for  E. coli  and readout probes for  P. aeruginosa  was added and acquisition of images was started (Image: 0 min, 2 min, 4 min, 8 min, 12 min). 
         FIG.  14    shows an overview of Example 11. Fixed monomicrobial stocks of  E. coli  are plated in different wells and encoded with a unique set of 24 probes that have several readout bits (at least one on-bit per round). After encoding, the bacteria undergo: washing of encoding probes, probe exchange with the addition of readout and exchange probes, wash of readout and exchange probes, and imaging. Each round will yield a potentially non-unique 10 bit (sub-) barcode. The readouts exchanges were performed in four rounds, with the first and final round having identical readout probes used for a recovery check. After the final round a full barcode (30 bits) can be generated. 
         FIG.  15    shows a basic design and concept for HiPR-Swap, in situ. A unique set of 30 readout probes were designed that can be used with a standard 10-bit system described herein. To achieve this, each oligo sequence on the readout probe is unique, but each fluorophore is used three times. Readout probes with the same fluorophore must be used in different rounds to achieve accurate barcode interpretation. As an example of their use, a schematic of bacteria and the encoding scheme is shown. The bacteria is encoded with probes targeting the rRNA and with flanking landing pads (colored) that correspond to the reverse complement of the intended readout probe. In each round a set of 10 readout probes (and possibly 10 exchange probes) are added to determine a sub-barcode for the round. After each round, the readout probes are removed from the specimen and a new batch is added. After all rounds are complete, classification is performed to determine the round-barcode for each cell. The round barcodes are then concatenated to determine the full barcode. 
         FIGS.  16 A- 16 B  provide a summary of classification accuracy for Example 11. Barcode classification was performed for each cell in each round. Each well was encoded with a unique barcode (legend in bottom right).  FIG.  16 A : The accuracy was defined as the number of cells with round-barcodes exactly matching the encoding (Match=TRUE) divided by the number with any difference from encoding (Match=FALSE). For each well, in each round, over 2000 bacterial cells were classified. A single cell was misclassified in round 3 of well 1.  FIG.  16 B : A fourth round of exchange was performed to restore the original, round 1 barcode in each well and again performed classification. The accuracy of classification for round 1 and round 4 for each well is shown. 
         FIG.  17    illustrates that bacteria fluorescence matches expected barcode. In each well a mask for the most abundant barcode applied to the maximum spectral projection. Fluorescent bacteria only appear in channels corresponding to the “1” bit. 
         FIG.  18    shows a field of view in tissue for three different rounds of HiPR-Swap to detect microbial taxa at the phylum level. For each round, the colors corresponding to the phyla present in the round are shown. Large speckled blue (color not shown) objects at the bottom of each image are DAPI-stained nuclei in the host epithelium. Insets with bacteria are shown in white boxes. Outline phylum names indicate low abundance taxa. 
         FIG.  19    shows a field of view in tissue for three different rounds of HiPR-Swap to detect microbial taxa at the species level. For each round, individual species were encoded with a single bit. Large speckled blue objects at the top right of each image are DAPI-stained nuclei in the host epithelium. Color change between images indicates signal exchange from the HiPR-Swap assay. 
     
    
    
     DETAILED DESCRIPTION 
     It is to be appreciated that certain aspects, modes, embodiments, variations, and features of the present methods and compositions are described below in various levels of detail in order to provide a substantial understanding of the present disclosure. 
     Definitions 
     Where values are described as ranges, endpoints are included. Furthermore, it will be understood that such disclosure includes the disclosure of all possible sub-ranges within such ranges, as well as specific numerical values that fall within such ranges irrespective of whether a specific numerical value or specific sub-range is expressly stated. 
     “5′-end” and “3′-end” refers to the directionality, e.g., the end-to-end orientation of a nucleotide polymer (e.g., DNA). The 5′-end of a polynucleotide is the end of the polynucleotide that has the fifth carbon. 
     The term “about,” as used herein, refers to +/−10% of a recited value. 
     “Complementary” refers to the topological compatibility or matching together of interacting surfaces of two nucleotides as understood by those of skill in the art. Thus, two sequences are “complementary” to one another if they are capable of hybridizing to one another to form a stable anti-parallel, double-stranded nucleic acid structure. A first nucleotide is complementary to a second nucleotide if the nucleotide sequence of the first nucleotide is substantially identical to the nucleotide sequence of the nucleotide binding partner of the second nucleotide, or if the first nucleotide can hybridize to the second nucleotide under stringent hybridization conditions. Thus, the nucleotide whose sequence is 5′-TATAC-3′ is complementary to a nucleotide whose sequence is 5′-GTATA-3′. 
     “Nucleotides,” “Nucleic acids,” “polynucleotide” or “oligonucleotide” refer to a polymeric-form of DNA and/or RNA (e.g., ribonucleotides, deoxyribonucleotides, or analogs thereof) of any length; e.g., a sequence of two or more ribonucleotides or deoxyribonucleotides. As used herein, the term “nucleotides” includes double- and single-stranded DNA, as well as double- and single-stranded RNA; it also includes modified and unmodified forms of a nucleotide (modifications to and of a nucleotide, for example, can include methylation, phosphorylation, and/or capping). In some embodiments, a nucleotide can be one of the following: a gene or gene fragment; genomic DNA; genomic DNA fragment; exon; intron; messenger RNA (mRNA); transfer RNA (tRNA); ribosomal RNA (rRNA); ribozyme; cDNA; recombinant nucleotide; branched nucleotide; plasmid; vector; isolated DNA of any sequence; isolated RNA of any sequence; any DNA described herein, any RNA described herein, primer or amplified copy of any of the foregoing. 
     In some embodiments, nucleotides can have any three-dimensional structure and may perform any function, known or unknown. The structure of nucleotides can also be referenced to by their 5′- or 3′-end or terminus, which indicates the directionality of the nucleotide sequence. Adjacent nucleotides in a single-strand of nucleotides are typically joined by a phosphodiester bond between their 3′ and 5′ carbons. However, different internucleotide linkages could also be used, such as linkages that include a methylene, phosphoramidate linkages, etc. This means that the respective 5′ and 3′ carbons can be exposed at either end of the nucleotide sequence, which may be called the 5′ and 3′ ends or termini. The 5′ and 3′ ends can also be called the phosphoryl (PO 4 ) and hydroxyl (OH) ends, respectively, because of the chemical groups attached to those ends. The term “nucleotides” also refers to both double- and single-stranded molecules. 
     In some embodiments, nucleotides can include modified nucleotides, such as methylated nucleotides and nucleotide analogs (including nucleotides with non-natural bases, nucleotides with modified natural bases such as aza- or deaza-purines, etc.). If present, modifications to the nucleotide structure can be imparted before or after assembly of the nucleotide sequence. 
     In some embodiments, the sequence of nucleotides can be interrupted by non-nucleotide components. One or more ends of the nucleotides can be protected or otherwise modified to prevent that end from interacting in a particular way (e.g. forming a covalent bond) with other nucleotides. 
     In some embodiments, nucleotides can be composed of a specific sequence of four nucleotide bases: adenine (A); cytosine (C); guanine (G); and thymine (T). Uracil (U) can also be present, for example, as a natural replacement for thymine when the nucleotide is RNA. Uracil can also be used in DNA. Thus, the term “sequence” refers to the alphabetical representation of nucleotides or any nucleic acid molecule, including natural and non-natural bases. 
     When used in terms of length, for example 20 nt, “nt” refers to nucleotides. 
     As used herein a “taxon” refers to a group of one or more populations of an organism or organisms. In some embodiments, a “taxon” refers to a phylum, a class, an order, a family, a genus, a species, or a train. In some embodiments, the disclosure includes providing a list of taxa of microorganisms. In some embodiments, the list of taxa of microorganisms is selected from a list of phyla, a list of classes, a list of orders, a list of families, a list of genera, or a list of species, of microorganisms. 
     In analysis of a sample, a species can be a target of interest. For example, a species can include a taxonomic species. 
     In the event of any term having an inconsistent definition between this application and a referenced document, the term is to be interpreted as defined herein. 
     The development of antimicrobial resistance among infectious organisms is an emerging problem in patient treatment. Some microbial organisms have even become resistant to multiple classes of antimicrobials, leading to increasing incidences of potentially fatal infections that cannot be treated with available antimicrobials. In some cases, microbes possessing more than one antimicrobial gene may only begin expressing one or more of these genes after exposure to antimicrobials. Currently, microbiology laboratories in hospitals and clinics rely on culturing bacteria from patient samples before species identification or antimicrobial susceptibility testing, however culturing bacteria is time-consuming and labor-intensive. Furthermore, many microorganisms are not readily culturable. 
     In a typical clinical lab workflow, the culturing step involves plating patient samples on an agar substrate and waiting for individual bacterium to grow into macroscopic colonies, each containing 10 to 100 million cells. Depending on the species of bacteria, this process can take between 24 hours to several days, which leads to a significant time delay from sampling to diagnosis. In addition, culturing bacteria requires a technician to prepare the culture plates by hand and evaluate bacterial growth by eye. Both of these factors add unnecessary hands-on time for the technician, and further increase the amount of time required for diagnosis. In certain classes of diseases such as sepsis, a delayed diagnosis can mean life or death for the patient. 
     The key innovative step of this method is to implement parallel single-cell imaging for microbial identification and characterization, which identification of microbial genera and species, and assessment of growth under different antimicrobial conditions directly on individual microbial cells or small colonies of cells, without the need to wait for cells to grow and divide into colonies containing millions to billions of cells. 
     Cell division events or microbial morphology changes can be monitored via iterative imaging of the sample during culture, or at the conclusion of culturing and following fixation, to measure microbial growth and stress in a solution with a given concentration of antimicrobials. Because the observation of only a few cell division events is sufficient to assess susceptibility or resistance of the microbial species to an antimicrobial agent, this technique can provide definitive results in less time than a complete cell cycle. This process is orders of magnitude faster than current techniques. For example, in a population of 1000 asynchronously dividing cells, the mean waiting time for the next division event to occur is 1/1000 of the duration of the typical cell cycle. For example, if the bacteria is  E. coli , with an average cell division time of 20 minutes, the next event occurs after roughly one second. The parallel observation of many (thousands and more) cells also enables the construction of division time distribution for accurate determination of growth rate over a time duration of one or few cell cycles. In some embodiments, the cells may be allowed to grow for a defined period of time. After the growth period, the samples can be fixed and observed on a microscope. In some cases, growth is measured by counting the number of micro colonies present in the sample. In other embodiments, the cells may be observed on a microscope while they are growing. In some embodiments, after acquiring the necessary growth and stress data, the sample can be fixed directly and parallel single-cell imaging performed to read out the species identity of the microorganism of interest. This may be followed up with single molecule imaging to measure the presence of genes that may indicate current or future susceptibility to antimicrobials. The micro-colony level or single-cell level observation will drastically cut down the time required to go from sample to diagnosis, requiring on average a few (e.g. one, two, or three) cell divisions to occur before the readout step, and will provide clinicians with actionable information earlier than any existing technology. Furthermore, the present methods provide clinicians with the antimicrobial susceptibility information needed to deploy targeted antimicrobials and enable precise treatments tailored for each individual case, thereby reducing the spread of multi-drug resistance among microbial populations. A live/dead stain (e.g. viability dye) can also be incorporated in unused spectral channels, to distinguish single, living microorganisms which did not divide over the course of the assay from those that are dead. 
     In addition to antimicrobial susceptibility, other microbial phenotype measurements can be combined with HiPR-FISH species identification and quantification. In some embodiments, the tolerance or persistence of microbial cells in the presence of environmental stress can be determined by measuring the gene expression levels for stress response genes (e.g. RpoS, RpoN, and/or RpoE, which encodes the sigma factor that regulates the response to conditions of stress). In some embodiments, motility or chemotaxis measurements can be combined with HiPR-FISH to identify cellular motility in a taxa-specific fashion. In some embodiments, the production of reactive oxygen species (ROS), which play important roles in promoting microbial tolerance to environmental stress, can be measured and linked to the species identity of each cell. In some embodiments, the expression of Type 3 Secretion System (T3 SS) genes, which are used by certain pathogens to infect host cells and evade host immune response, can be measured and linked to species identity. In some embodiments, the expression of Type IV Secretion System (T4SS), which is related to the prokaryotic conjugation machinery and is involved in transport of proteins and DNA across the cell membrane, can be measured and linked to species identity. In some embodiments, the expression of quorum sensing genes, which are important in modulating collective behavior of communities containing many microbial cells, can be measured and linked to species identity. In some embodiments, the expression of genes related to biofilm formation can be measured and linked to species identity. In some embodiments, microbial cells can be subjected to a phage to identify phage-susceptible microbial species. 
     Single Cell Imaging 
     In some embodiments, the present disclosure is directed to a method that achieves high phylogenetic resolution by taking advantage of the abundance of existing ribosomal subunit sequence information, such as the 16S ribosomal RNA sequence information, and a highly multiplexed binary encoding scheme. In some embodiments, each taxon from a list of taxa of microorganisms is probed with a custom designed taxon-specific targeting sequence, flanked by a subset of n unique encoding sequences. In some embodiments, each taxon is assigned a unique n-bit binary word, where 1 or 0 at the i th  bit indicates the taxon-specific targeting sequence is flanked or not flanked by the i h  encoding sequence. In some embodiments, a mixture of n decoding probes, each complementary to one of the n encoding sequences and conjugated to a unique label, is allowed to hybridize to their complementary encoding sequences. In some embodiments, the spectrum of labels for each cell is then detected using spectral imaging techniques. In some embodiments, the barcode identity for each cell can then be assigned using a support vector machine, using spectra of cells encoded with known barcodes or using computationally simulated spectra as training data. 
     In some embodiments, each taxon from a list of taxa of microorganisms is assigned a unique n-bit binary code selected from a plurality of unique n-bit binary codes, where n is an integer greater than 1. 
     A “binary code” refers to a representation of taxa using a string made up of a plurality of “0” and “1” from the binary number system. The binary code is made up of a pattern of n binary digits (n-bits), where n is an integer representing the number of labels used. The bigger the number n, the greater number of taxa can be represented using the binary code. For example, a binary code of eight bits (an 8-bit binary code, using 8 different labels) can represent up to 255 (2 8 −1) possible taxa. (One is subtracted from the total possible number of codes because no taxon is assigned a code of all zeros “00000000.” A code of all zeros would mean no decoding sequence, and thus no label, is attached. In other words, there are no non-labeled taxa.) Similarly, a binary code of ten bits (a 10-bit binary code) can represent up to 1023 (2 10 −1) possible taxa. In some embodiments a binary code may be translated into and represented by a decimal number. For example, the 10-bit binary code “0001100001” can also be represented as the decimal number “97.” 
     Each digit in a unique binary code represents whether a readout probe and the fluorophore corresponding to that readout probe are present for the selected species. In some embodiments, each digit in the binary code corresponds to a Readout probe (from Readout probe 1 (R1) through Readout probe n (Rn) in an n-bit coding scheme). In a specific embodiment, the n is 10 and the digits of an n-bit code correspond to R1 through R10. In some embodiments, the fluorophores that correspond to R1 through Rn are determined arbitrarily. For example, if n is 10, R1 can correspond to an Alexa 488 fluorophore, R2 can correspond to an Alexa 546 fluorophore, R3 can correspond to a 6-ROX (6-Carboxy-X-Rhodamine, or Rhodamine Red X) fluorophore, R4 can correspond to a Pacific Green fluorophore, R5 can correspond to a Pacific Blue fluorophore, R6 can correspond to an Alexa 610 fluorophore, R7 can correspond to an Alexa 647 fluorophore, R8 can correspond to a DyLight-510-LS fluorophore, R9 can correspond to an Alexa 405 fluorophore, and R10 can correspond to an Alexa532 fluorophore. Other n-bit and readout probes combinations are also contemplated herein. In some embodiments, other fluorophores including, but not limited to Hydroxycoumarin, methoxycoumarin, Cy2, FAM, Flourescein FITC, Alexa 430, R-phycoerythrin (PE), Tamara, Cy3.5 581, Rox, Alexa fluor 568, Red 613, Texas Red, Alexa fluor 594, Alexa fluor 633, Alexa fluor 660, Alexa fluor 680, Cy5, Cy 5.5, Cy 7, and Allophycocyanin are used in the n-bit encoding system. 
     In some embodiments, the n-bit binary code is between a 2-bit binary code and 50-bit binary code, a 2-bit binary code and 40-bit binary code, or 2-bit binary code and 30-bit binary code. In some embodiments, the n-bit binary code is selected from the group consisting of 2-bit binary code, 3-bit binary code, 4-bit binary code, 5-bit binary code, 6-bit binary code, 7-bit binary code, 8-bit binary code, 9-bit binary code, 10-bit binary-code, 11-bit binary code, 12-bit binary code, 13-bit binary code, 14-bit binary code, 15-bit binary code, 16-bit binary code, 17-bit binary code, 18-bit binary code, 19-bit binary code, 20-bit binary code, 21-bit binary code, 22-bit binary code, 23-bit binary code, 24-bit binary code, 25-bit binary code, 26-bit binary code, 27-bit binary code, 28 bit binary code, 29-bit binary code, 30-bit binary code, 31-bit binary code, 32-bit binary code, 33-bit binary code, 34-bit binary code, 35-bit binary code, 36-bit binary code, 37-bit binary code, 38 bit binary code, 39-bit binary code, 40-bit binary code, 41-bit binary code, 42-bit binary code, 43-bit binary code, 44-bit binary code, 45-bit binary code, 46-bit binary code, 47-bit binary code, 48 bit binary code, 49-bit binary code, and 50-bit binary code. 
     Encoding Probes 
     In some embodiments, the gene for a ribosomal subunit is used as a marker for phylogenetic placement. In some embodiments, 16S rRNA gene is used as a marker for phylogenetic placement. In some embodiments, methods of the present disclosure comprise multiplexed in-situ hybridization of encoding probes targeting taxon-specific segments of multiple unique 16S rRNA genes present in a microorganism population. In some embodiments, the 5S and/or 23S rRNA are used independently or in conjunction with 16S rRNA as a marker for phylogenetic placement. In some embodiments, if non-bacterial microorganisms are targeted, other rRNA may be targeted. 
     In some embodiments, a set of ending probes comprises subsets of encoding probes, wherein each subset targets a specific taxon. In some embodiments, a subset of encoding probes contains one unique targeting sequence specific to a taxon; that is, the encoding probes within a subset share a common targeting sequence specific to a taxon. In some embodiments, a subset of encoding probes contains multiple unique targeting sequences, each unique targeting sequence being specific to the same taxon as other targeting sequences within the same subset. 
     Targeting Sequences 
     In some embodiments, each encoding probe comprises a targeting sequence which is substantially complementary to a taxon-specific 16S rRNA sequence. By “substantially complementary” it is meant that the nucleic add fragment is capable of hybridizing to at least one nucleic acid strand or duplex even if less than all nucleobases base pair with a counterpart nucleobase. In certain embodiments, a “substantially complementary” nucleic add contains at least one sequence in which about 70%, about 71%, about 72%, about 73%, about 74%, about 75%, about 76%, about 77%, about 77%, 8%, about 79%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, to about 100%, and any range therein, of the nucleobase sequence is capable of basepairing with at least one single or double stranded nucleic acid molecule during hybridization. 
     In some embodiments, the targeting sequence is designed to have a predicted melting temperature of between about 45° C. and about 65° C. or between about 55° C. and about 65° C. As used herein, the term “about” refers to an approximately ±10% variation from a given value. In some embodiments, the predicted melting temperature of the targeting sequence is 45° C., 46° C., 47° C., 48° C., 49° C., 50° C., 51° C., 52° C., 53° C., 54° C., 55° C., 56° C., 57° C., 58° C., 59° C., 60° C., 61° C., 62° C., 63° C., 64° C. or 65° C. In some embodiments, the targeting sequence has a GC content of about 55%, 60%, 65% or 70%. 
     In some embodiments, the taxon-specific targeting sequence in an encoding probe is designed as follows. At first, 16S sequences from a plurality of microorganisms are grouped by taxon and sequence similarity and a consensus sequence is generated for each taxon. In some embodiments, a targeting sequence specific for a consensus sequence is at least 10 nucleotides to at least 100 nucleotides long. In some embodiments, a targeting sequence specific for a consensus sequence is at least 15 nucleotides long, at least 16 nucleotides long, at least 17 nucleotides long, at least 18 nucleotides long, at least 19 nucleotides long, at least 20 nucleotides long, at least 21 nucleotides long, at least 22 nucleotides long, at least 23 nucleotides long, at least 24 nucleotides long, at least 25 nucleotides long, at least 26 nucleotides long, at least 27 nucleotides long, at least 28 nucleotides long, at least 29 nucleotides long, at least 30 nucleotides long, at least 35 nucleotides long, at least 40 nucleotides long, at least 45 nucleotides long, or at least 50 nucleotides long. In some embodiments, the candidate targeting sequence is aligned against a catalog of all full-length 16S rRNA sequences of a list of microorganisms. In a specific embodiment, the alignment is performed using Blastn (NCBI). In a specific embodiment, the alignment is performed using BWA. In a specific embodiment, the alignment is performed using bowtie. In a specific embodiment, the alignment is performed using bowtie2. In some embodiments, a maximum continuous homology (MCH) score, defined as the maximum number of continuous bases that are shared between the query and the target sequence, is calculated for each blast hit. In some embodiments, only candidate targeting sequences having blast hits to the consensus sequence above a threshold MCH score are considered significant and used for further analysis. In some embodiments, a blast on-target rate, defined as the ratio between the number of correct blast hits and the total number of significant blast hits, is calculated for each candidate targeting sequence having a significant BLAST hit. In some embodiments, any candidate targeting sequence with a blast on-target rate of less than 1 is excluded from the probe set to avoid ambiguity, and the remaining candidate targeting sequences are used as targeting sequences in encoding probe synthesis. 
     In some embodiments, the targeting sequence of an encoding probe is designed using publicly-available 16S rRNA sequence data. In some embodiments, the targeting sequence of an encoding probe is designed using publicly-available 23S rRNA sequence data. In some embodiments, the targeting sequence of an encoding probe is designed using publicly-available 5S rRNA sequence data. In some embodiments, the targeting sequence of an encoding probe design is designed using custom catalogues of 16S rRNA sequences. In some embodiments, the targeting sequence of an encoding probe design is designed using custom catalogues of 23S rRNA sequences. In some embodiments, the targeting sequence of an encoding probe design is designed using custom catalogues of 5S rRNA sequences. In some embodiments, the targeting sequence of an encoding probe is designed using publicly-available 16S-5S rRNA sequence data. In some embodiments, the targeting sequence of an encoding probe is designed using publicly-available 16S-5S-23S rRNA sequence data. In some embodiments, the targeting sequence of an encoding probe design is designed using custom catalogues of 16S-5S rRNA sequences. In some embodiments, the targeting sequence of an encoding probe design is designed using custom catalogues of 16S-5S-23S rRNA sequences. In a specific embodiment, high-quality, full-length 16S sequences are obtained by circular consensus sequencing (SMRT-CCS). In a specific embodiment, high-quality, full-length 16S sequences are obtained by Nanopore sequencing. 
     In some embodiments, SMRT-CCS of a 16S ribosomal sequence involves isolating ribosomal DNA from a microorganism. In a specific embodiment, DNA isolation is achieved using QIAamp DNA Mini Kit. In a specific embodiment, DNA isolation is achieved using DNeasy PowerSoil Pro Kit. In some embodiments, ribosomal DNA is amplified using universal primers. In some embodiments, the amplified ribosomal DNA is purified, and sequenced. In a specific embodiment, sequencing is performed on a PacBio Sequel instrument. In a specific embodiment, sequencing is performed on a PacBio Sequel IIe instrument. In a specific embodiment, sequencing is performed on a Nanopore MinION instrument. In a specific embodiment, sequencing is performed on a Nanopore GridION instrument. In a specific embodiment, sequencing is performed on a Nanopore PromethION instrument. In some embodiments, sequence data is processed to create a circular consensus sequence with a threshold of 99% accuracy. In a specific embodiment, the sequence data processing is achieved using rDnaTools. In some embodiments, the circular consensus sequences are used for probe design. In some embodiments, to increase the sequence design space, and to improve identification of closely related species, the workflow uses a full 16S-23S rRNA region. In some embodiments, to increase the sequence design space, and to improve identification of closely related species, the workflow uses a full 16S-5S-23S rRNA region. 
     In some embodiments, the targeting sequence of an encoding probe is designed using a database that is relevant for a system. In a specific embodiment, the system is the gut microbiome. In some embodiments, the targeting sequence of an encoding probe is designed using a database that is relevant for a disease or infection. 
     Spacers 
     In some embodiments, a targeting sequence in an encoding probe is concatenated on both ends with 3 nucleotide (3-nt) spacers. In some embodiments, the 3-nt spacers comprise a random string of three nucleotides. In some embodiments, the 3-nt spacers are sequences designed from the 16S rRNA molecule, 5S rRNA molecule, or 23S rRNA molecule (i.e., three nucleotides upstream and downstream of the selected 16S targeting sequence is used as the 3-nt spacers). In some embodiments the spaces are non-nucleotide chemical spacers. Non-nucleotide chemical spacers include, but are not limited to, hexanediol, hexa-ethyleneglycol, or triethylene glycol spacers. 
     Readout Sequences 
     In some embodiments, a targeting sequence is concatenated to at least one readout sequence depending on the unique n-bit binary code assigned to the taxon that the targeting sequence is specific for. Each readout sequence is substantially complementary to the sequence of a corresponding labeled readout probe. 
     In some embodiments, a readout sequence is at least 15 nucleotides long, at least 16 nucleotides long, at least 17 nucleotides long, at least 18 nucleotides long, at least 19 nucleotides long, at least 20 nucleotides long, at least 21 nucleotides long, at least 22 nucleotides long, at least 23 nucleotides long, at least 24 nucleotides long, at least 25 nucleotides long, at least 26 nucleotides long, at least 27 nucleotides long, at least 28 nucleotides long, at least 29 nucleotides long, or at least 30 nucleotides long. In some embodiments, candidate readout sequences are blasted against a nucleotide database to ensure that they are not substantially complementary to regions of 16S ribosomal sequences. 
     Forward and Reverse Primers 
     In some embodiments, a targeting sequence is concatenated to a set of sequences (forward primer and reverse primer sequences) that are substantially complementary to primers that can be used to amplify the encoding probe in a polymerase chain reaction (PCR). In some embodiments, the forward and reverse primers are designed to have predicted melting temperatures of between about 55° C. and about 65° C. As used herein, the term “about” refers to an approximately ±10% variation from a given value. In some embodiments, the predicted melting temperature of the forward and reverse primers are 55° C., 56° C., 57° C., 58° C., 59° C., 60° C., 61° C., 62° C., 63° C., 64° C. or 65° C. In some embodiments, the forward and reverse primers have a GC content of about 55%, 60%, 65% or 70%. 
     In some embodiments, the set of forward and reverse primers are designed such that the set of forward and reverse primers are not substantially complementary to the targeting sequence or readout sequences. In some embodiments, the set of forward and reverse primers are designed such that the set of forward and reverse primers are not substantially complementary to any sequences that are substantially complementary to the targeting sequence or readout sequences. In a specific embodiment, the set of forward primer and reverse primer sequences comprise the nucleotide sequence CGATGCGCCAATTCCGGTTC (SEQ ID NO: 1808) and the nucleotide sequence GTCTATTTTCTTATCCGACG (SEQ ID NO: 1809). 
     In some embodiments, the forward primer or the reverse primer is at least 15 nucleotides long, at least 16 nucleotides long, at least 17 nucleotides long, at least 18 nucleotides long, at least 19 nucleotides long, at least 20 nucleotides long, at least 21 nucleotides long, at least 22 nucleotides long, at least 23 nucleotides long, at least 24 nucleotides long, at least 25 nucleotides long, at least 26 nucleotides long, at least 27 nucleotides long, at least 28 nucleotides long, at least 29 nucleotides long, or at least 30 nucleotides long. 
     Decoding Probes 
     In some embodiments, the present disclosure utilizes a set of n number of decoding probes representing an n-bit coding scheme where n is an integer. In some embodiments, each probe in the set of decoding probes corresponds to a digit in the plurality of unique n-bit binary codes. 
     In some embodiments, each probe in the set of decoding probes is conjugated with a label that provides a detectable signal. 
     In some embodiments, each probe in a set of decoding probes is labeled different from other probes in the set, and each decoding probe is substantially complementary to a corresponding readout sequence selected from a set of n number of readout sequences. 
     In some embodiments, the detectable signal is a cyanine dye (e.g., Cy2, Cy3, Cy3B, Cy5, Cy5.5, Cy7, etc.), Alexa Fluor dye, Atto dye, photo switchable dye, photoactivatable dye, fluorescent dye, metal nanoparticle, semiconductor nanoparticle or “quantum dots”, fluorescent protein such as GFP (Green Fluorescent Protein), or photoactivatable fluorescent protein, such as PAGFP, PSCFP, PSCFP2, Dendra, Dendra2, EosFP, tdEos, mEos2, mEos3, PAmCherry, PAtagRFP, mMaple, mMaple2, and mMaple3. 
     In a specific embodiment, the detectable signal is a fluorophore. In some embodiments, the detectable signal is a fluorophore that emits light in infrared or near-infrared. In a specific embodiment, the fluorophore is selected from the group consisting of Alexa 405, Pacific Blue, Pacific Green, Alexa 488, Alexa 532, Alexa 546, Rhodamine Red X, Alexa 610, Alexa 647, and DyLight-510-LS, Hydroxycoumarin, methoxycoumarin, Cy2, FAM, Fluorescein FITC, Alexa 430, R-phycoerythrin (PE), Tamara, Cy3.5 581, Rox, Alexa fluor 568, Red 613, Texas Red, Alexa fluor 594, Alexa fluor 633, Alexa fluor 660, Alexa fluor 680, Cy5, Cy5.5, Cy7, Allophycocyanin, and ROX (carboxy-X-rhodamine). In some embodiments, the detectable signal is Alexa Fluor 350, Alexa Fluor 405, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 514, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 561, Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 610, Alexa Fluor 633, Alexa Fluor 635, Alexa Fluor 647, Alexa Fluor 647-R-phycoerythrin, Alexa Fluor 660, Alexa Fluor 680, Alexa Fluor 680-allophycocyanin, Alexa Fluor 700, Alexa Fluor 750, Alexa Fluor 790, Alexa Fluor Plus 405, Alexa Fluor Plus 488, Alexa Fluor Plus 555, Alexa Fluor Plus 594, Alexa Fluor Plus 647, Alexa Fluor Plus 680, Alexa Fluor Plus 750, Alexa Fluor Plus 800, Pacific Blue, Pacific Green, Rhodamine Red X, DyLight 485-LS, DyLight-510-LS, DyLight 515-LS, DyLight 521-LS, Hydroxycoumarin, methoxycoumarin, Cy2, FAM, Fluorescein FITC, R-phycoerythrin (PE), Tamara, Cy3.5 581, ROX (carboxy-X-rhodamine), Red 613, Texas Red, Cy5, Cy5.5, Cy7, Allophycocyanin, ATTO 430LS, ATTO 490LS, ATTO 390, ATTO 425, Cyan 500 NHS-Ester, ATTO 465, ATTO 488, ATTO 495, ATTO Rho110, ATTO 514, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 542, ATTO 550, ATTO 565, ATTO Rho3B, ATTO Rho11, ATTO Rho12, ATTO Thio12, ATTO Rho101, ATTO 590, ATTO 594, ATTO Rho13, ATTO 610, ATTO 620, ATTO Rho14, ATTO 633, ATTO 643, ATTO 647, ATTO 647N, ATTO 655, ATTO Oxa12, ATTO 665, ATTO 680, ATTO 700, ATTO 725, ATTO 740. 
     In some embodiments, a readout probe is at least 10 nucleotides long, at least 11 nucleotides long, at least 12 nucleotides long, at least 13 nucleotides long, at least 14 nucleotides long, at least 15 nucleotides long, at least 16 nucleotides long, at least 17 nucleotides long, at least 18 nucleotides long, at least 19 nucleotides long, at least 20 nucleotides long, at least 21 nucleotides long, at least 22 nucleotides long, at least 23 nucleotides long, at least 24 nucleotides long, at least 25 nucleotides long, at least 26 nucleotides long, at least 27 nucleotides long, at least 28 nucleotides long, at least 29 nucleotides long, or at least 30 nucleotides long. 
     Imaging 
     In some embodiments, the labels used in the present methods are imaged using a microscope. In some embodiments, the microscope is a confocal microscope. In some embodiments, the microscope is a fluorescence microscope. In some embodiments, the microscope is a light-sheet microscope. In some embodiments, the microscope is a super-resolution microscope. 
     Barcode Decoding 
     In some embodiments, a support vector machine is trained on reference data to predict the barcode of single cells in the synthetic communities and environmental samples. In a specific embodiment, the support vector machine is Support Vector Regression (SVR) from Python package. As used herein, the term “support-vector machine” (SVM) refers to a supervised learning model with associated learning algorithms that analyze data used for classification and regression analysis. Given a set of training examples, each marked as belonging to one or the other of two categories, an SVM training algorithm builds a model that assigns new examples to one category or the other, making it a non-probabilistic binary linear classifier. An SVM model is a representation of the examples as points in space, mapped so that the examples of the separate categories are divided by a clear gap that is as wide as possible. New examples are then mapped into that same space and predicted to belong to a category based on which side of the gap they fall. 
     In some embodiments, the reference spectra are obtained through a brute force approach involving the measurement of the spectra of all possible barcodes using barcoded test  E. coli  cells. In some embodiments, the n-bit binary encoding is a 10-bit binary encoding and tire reference spectra are obtained through measuring 1023 reference spectra. 
     In some embodiments the reference spectra are obtained by simulation of all possible spectra. In some embodiments, the simulated spectral data can be used as reference examples for the support vector machine. In some embodiments, the spectra corresponding to individual n-bit binary codes are simulated by adding together the measured spectra of each individual fluorophore (e.g., the reference spectrum for 0000010011 is generated by adding the spectra of R1, R2, and R5; or the reference spectrum for 1010010100 is generated by adding the spectra of R3, R5, R8 and R10). In some embodiments, the spectra corresponding to individual n-bit binary codes are simulated by adding the measured spectra of each individual fluorophore weighted by the relative contribution to the emission signal of each fluorophore. In some embodiments, the relative contribution of each fluorophore is calculated using a Forster Resonant Energy Transfer (FRET) model. 
     In one aspect, the disclosure is directed to a computer-readable storage device storing computer readable instructions, which when executed by a processor causes the processor to assign each taxon in a list of taxa of microorganisms a unique n-bit binary code selected from a plurality of unique n-bit binary codes, and design decoding and encoding probes suitable for use in such n-bit binary coding scheme. 
     The phrase “computer-readable storage device” refers to a computer readable storage device or a computer readable signal medium. A computer-readable storage device, may be, for example, a magnetic, optical, electronic, electromagnetic, infrared, or semiconductor system, apparatus, or device, or any suitable combination of the foregoing; however, the computer readable storage device is not limited to these examples except a computer readable storage device excludes computer readable signal medium Additional examples of the computer readable storage device can include: a portable computer diskette, a hard disk, a magnetic storage device, a portable compact disc read-only memory (CD-ROM), a random access memory&#39; (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or Flash memory), an optical storage device, or any appropriate combination of the foregoing; however, the computer readable storage device is also not limited to these examples. 
     Any tangible medium that can contain, or store, a program for use by or in connection with an instruction execution system, apparatus, or device could be a computer readable storage device. 
     A computer readable signal medium may include a propagated data signal with computer readable program code embodied therein, such as, but not limited to, in baseband or as part of a carrier wave. A propagated signal may take any of a plurality of forms, including, but not limited to, electro-magnetic, optical, or any suitable combination thereof. A computer readable signal medium may be any computer readable medium (exclusive of computer readable storage device) that can communicate, propagate, or transport a program for use by or in connection with a system, apparatus, or device. Program code embodied on a computer readable signal medium may be transmitted using any appropriate medium, including but not limited to wireless, wired, optical fiber cable, RF, etc., or any suitable combination of the foregoing. The term “memory” as used herein comprises program memory&#39; and working memory. The program memory may have one or more programs or software modules. The working memory stores data or information used by the CPU in executing the functionality described herein. 
     The term “processor” may include a single core processor, a multi-core processor, multiple processors located in a single device, or multiple processors in wired or wireless communication with each other and distributed over a network of devices, the Internet, or the cloud. Accordingly, as used herein, functions, features or instructions performed or configured to be performed by a “processor”, may include the performance of the functions, features or instructions by a single core processor, may include performance of the functions, features or instructions collectively or collaboratively by multiple cores of a multi-core processor, or may include performance of the functions, features or instructions collectively or collaboratively by multiple processors, where each processor or core is not required to perform every function, feature or instruction individually. The processor may be a CPU (central processing unit). The processor may comprise other types of processors such as a GPU (graphical processing unit). In other aspects of the disclosure, instead of or in addition to a CPU executing instructions that are programmed in the program memory, the processor may be an ASIC (application-specific integrated circuit), analog circuit or other functional logic, such as a FPGA (field-programmable gate array), PAL (Phase Alternating Line) or PLA (programmable logic array). 
     The CPU is configured to execute programs (also described herein as modules or instructions) stored in a program memory to perform the functionality described herein. The memory may be, but not limited to, RAM (random access memory), ROM (read only memory) and persistent storage. The memory is any piece of hardware that is capable of storing information, such as, for example without limitation, data, programs, instructions, program code, and/or other suitable information, either on a temporary basis and/or a permanent basis. 
     In some embodiments, a computer-readable storage device comprises instructions for assigning each taxon in a list of taxa of microorganisms a unique n-bit binary code selected from a plurality of unique n-bit binary codes; designing a set of n number of decoding probes, wherein each decoding probe corresponds to a digit in the n-bit binary code, and where each decoding probe is substantially complementary to a readout sequence selected from a set of n number of readout sequences, and designing a set of encoding probes, where the set of encoding probes includes a plurality of subsets of encoding probes, wherein each encoding probe comprises a targeting sequence and one or more readout sequences, the encoding probes within each subset comprise a targeting sequence that is specific to a taxon in tire list of taxa of microorganisms and is different from a targeting sequence of the encoding probes of another subset, and the readout sequences in the encoding probes within a subset are selected from the set of n number of readout sequences based on the unique n-bit binary code assigned to the taxon which the targeting sequence of the subset is specific to. 
     In some embodiments, the computer-readable storage device comprises instructions for designing encoding probes of a subset, wherein the targeting sequence in the encoding probes of a subset is substantially complementary to a consensus 16S ribosomal sequence specific to a taxon. In some embodiments, the computer-readable storage device comprises instructions for designing encoding probes of a subset, wherein the targeting sequence in the encoding probes of a subset is substantially complementary to a consensus 5S ribosomal sequence specific to a taxon. In some embodiments, the computer-readable storage device comprises instructions for designing encoding probes of a subset, wherein the targeting sequence in the encoding probes of a subset is substantially complementary to a consensus 23S ribosomal sequence specific to a taxon. In some embodiments, the computer-readable storage device comprises instructions for designing encoding probes of a subset, wherein the targeting sequence in the encoding probes of a subset is substantially complementary to a consensus 16S-5S ribosomal sequence specific to a taxon. In some embodiments, the computer-readable storage device comprises instructions for designing encoding probes of a subset, wherein the targeting sequence in the encoding probes of a subset is substantially complementary to a consensus 16S-5S-23S ribosomal sequence specific to a taxon. In some embodiments, the computer-readable storage device comprises instructions for designing encoding probes of a subset, wherein the targeting sequence in the encoding probes of a subset is substantially complementary to a consensus 16S-23S ribosomal sequence specific to a taxon. In some embodiments, the targeting sequence is blasted against a nucleotide database to ensure that the target sequence is not substantially complementary to any sequence other than the consensus 16S ribosomal sequence to which the target sequence is specific. 
     In some embodiments, a set of encoding probes comprises subsets of encoding probes, wherein each subset targets a specific taxon. In some embodiments, a subset of encoding probes contains one unique targeting sequence specific to a taxon; that is, the encoding probes within a subset share a common targeting sequence specific to a taxon. In some embodiments, a subset of encoding probes contains multiple unique targeting sequences, each unique targeting sequence being specific to the same taxon as other targeting sequences within the same subset. 
     Microbial Cell Growth 
     In some embodiments, the microbial cell in the sample is identified and characterized directly from the sample. In some embodiments, the microbial cell in the sample is identified and characterized after culturing. In some embodiments, the microbial cell in the sample is cultured for numerous cell divisions. A skilled artisan would readily recognize that the number of cell divisions depends on the species doubling time, which varies from species to species. In some embodiments, the microbial cell in the sample is cultured for one to numerous cell divisions. In some embodiments, the microbial cell in the sample is cultured for less than one division cycle. In some embodiments, the microbial cell in the sample is cultured for very few cell division cycles. In some embodiments, the microbial cell in the sample is cultured for about 1 to about 12 cell division cycles. In some embodiments, the microbial cell in the sample is cultured for about 1, about 2, about 3, about 4, about 5, about 6, about 7, about 8, about 9, about 10, about 11, or about 12 cell division cycles. In some embodiments, the microbial cell in the sample is cultured for about 1 minute to about 12 hours. In some embodiments, the microbial cell in the sample is cultured for about 1 minute, about 2 minutes, about 3 minutes, about 4 minutes, about 5 minutes, about 6 minutes, about 7 minutes, about 8 minutes, about 9 minutes, about 10 minutes, about 11 minutes, about 12 minutes, about 13 minutes, about 15 minutes, about 16 minutes, about 17 minutes, about 18 minutes, about 19 minutes, about 20 minutes, about 25 minutes, about 30 minutes, about 35 minutes, about 40 minutes, about 45 minutes, about 50 minutes, about 55 minutes, about 60 minutes, about 80 minutes, about 90 minutes, about 1 hour, about 1.5 hours, about 2 hours, about 2.5 hours, about 3 hours, about 3.5 hours, about 4 hours, about 4.5 hours, about 5 hours, about 5.5 hours, about 6 hours, about 6.5 hours, about 7 hours, about 7.5 hours, about 8 hours, about 8.5 hours, about 9 hours, about 9.5 hours, about 10 hours, about 10.5 hours, about 11 hours, about 11.5 hours, or about 12 hours. 
     Antimicrobial Susceptibility Testing 
     To enable rapid antimicrobial resistance profiling, the present methods combine fluorescence in situ hybridization to enable the first hybrid measurements of antimicrobial resistance ( FIG.  1 A ) using both genotypic and phenotypic information.  FIG.  1 A  shows a concept for rapid phenotypic profiling of antibiotic resistance followed by microbial identification using imaging. Microbes are cultured for a short amount of time (minutes) before fixation and imaging. Multimodal imaging using single-molecule FISH and metabolic labeling can provide phenotypic and genotypic information on cellular metabolism and antimicrobial resistance. This technique allows identification of microorganism species and assessment of microorganism growth and replication in the presence and absence of known concentrations of different antimicrobials in order to accurately determine antimicrobial susceptibility testing results in less time required than other methods known in the art. 
     One or more microbes in a specimen can be directly inoculated onto a device with patterned compartments. The testing can proceed with or without further culturing. In scenarios where the sample is not subjected to culturing, species identification FISH methods, such as HiPR-FISH, and single-molecule FISH to simultaneously image the species identity is combined with analysis regarding the presence or absence of one or more antimicrobial genes and metabolites, proteins, carbohydrates, and/or lipids in the same cells. This approach will enable a paired readout of microbial species identity and antimicrobial susceptibility. In situations where phenotypic readout of antimicrobial susceptibility is desired, the compartments will be filled with a culturing media containing an antimicrobial drug at a known concentration. An initial image will be taken to record the number of cells in each compartment of the device. The microbes are allowed to replicate for a defined period of time (minutes to a few hours). After the growth period, another image or measurement will be taken to record cellular state in each compartment of the device after the growth period and look for the presence of genes and metabolites, proteins, carbohydrates, and/or lipids that are known to confer antimicrobial resistance. The cellular state can potentially be read out in a few different ways. For example, cellular state can be measured simply by counting the number of cells in each compartment. Cell growth can also be measured by probing the metabolic product concentration in the solution such as dissolved CO 2  or measuring the amount of heat dissipation using calorimetry techniques. Cellular state can also be inferred by measuring the abundance of expressed metabolic genes or stress response genes using single-molecule fluorescence in situ hybridization. Cellular state may also be measured using a simple live/dead stain. After cellular state measurement, the identity of the cells will subsequently be read out using multiplexed fluorescence in situ hybridization (e.g. HiPR-FISH) ( FIG.  1 B ). Binary labeling approach for highly multiplexed labeling of microbes for taxonomic identification. Microbes from different taxa are labeled with unique combinations of fluorophores. The combined spectra are measured using a microscope in spectral imaging mode. Measured spectra are classified using a custom machine learning algorithm. This test can be repeated in several different culture media to make the analysis as comprehensive as possible. Altogether, the present methods enable rapid measurement of pathogen identity, their associated minimally inhibitory concentration for antimicrobials, and potential future susceptibility to antimicrobials. 
     In some aspects, the present disclosure provides methods determining the susceptibility (or resistance) of the microbial cells in the sample to one or more antimicrobial agents. In some aspects, the present disclosure provides methods of identifying microbial cells in a sample in parallel with determination of the microbial cells in the sample susceptibility to one or more antimicrobial agents. As used herein, a microbial cell is “susceptible” to an antimicrobial when it is inhibited by the usually achievable concentration of the antimicrobial agent when the dosage recommended to treat the site of infection is used. Further, as used herein, a microbial cell is “resistant” to an antimicrobial when it is not inhibited by the usually achievable concentration of an antimicrobial agent with normal dosage schedules and/or that has a minimum inhibitory concentration that falls in the range in which specific microbial resistance mechanisms are likely. 
     In some embodiments, the microbial cells in a sample are exposed to different concentrations to determine the minimum inhibitory concentration of the antimicrobial agent. In some embodiments, the minimum inhibitory concentration (MIC) of the antimicrobial agent for the microbial cell in the sample is greater than the MIC of a typical microbial cell of the same strain. In some embodiments, the minimum inhibitory concentration (MIC) of the antimicrobial agent for the microbial cell in the sample is lower than the MIC of a typical microbial cell of the same strain. 
     In some embodiments, the microbial cells in the sample are exposed to one or more antimicrobial agents in a concentration range of about 2-fold to about 500-fold of the MIC of a typical microbial cell of the same strain. In some embodiments, the microbial cells in the sample are exposed to one or more antimicrobial agents in a concentration range of about 2-fold, about 3-fold, about 4-fold, about 5-fold, about 6-fold, about 7-fold, about 8-fold, about 9-fold, about 10-fold, about 11-fold, about 12-fold, about 13-fold, about 14-fold, about 15-fold, about 16-fold, about 17-fold, about 18-fold, about 19-fold, about 20-fold, about 25-fold, about 30-fold, about 35-fold, about 40-fold, about 45-fold, about 50-fold, about 60-fold, about 70-fold, about 80-fold, about 90-fold, about 100-fold, about 150-fold, about 200-fold, about 250-fold, about 300-fold, about 350-fold, about 400-fold, about 450-fold, or about 500-fold of the MIC of a typical microbial cell of the same strain. 
     Any appropriate antimicrobial agent effective against a microbial cell disclosed herein may be used in the methods of the present disclosure. In some embodiments, the one or more antimicrobial agents include, but are not limited to rifamycins, rifampicin, aminoglycosides, fluoroquinolones, penicillins, carbapenems, cephalosporins antibiotic, penicillinase-resistant penicillins, aminopenicillins, β-lactams, tetracyclines, sulfonamides, phenicols, trimethoprim, macrolides, fosfomycin, erythromycin, azithromycin, clarithromycin, dirithromycin, troleandomycin, synthetic drugs quinolones, sulfonamides, trimethoprim, sulfamethoxazole, streptomycin, glycopeptides, glycylcyclines, ketolides, lipopeptides, monobactams, nitroimidazoles, oxazolidinones, polymixins, benzilpenicilline, aminoglycosides, amikacin, arbekacin, gentamicin, kanamycin, neomycin, netilmicin, paromomycin, rhodostreptomycin, streptomycin, tobramycin, apramycin, amphotericin, nystatin, pimaricin, fluconazole, itraconazole, voriconazole, posaconazole, isavuconazole, ketoconazole, echinocandins, polyenes, allylamines, naftifine, terbinafine, morpholines, amorolfine, 5-fluorocytosine, atovaquone/proguanil, malarone, chloroquine, doxycycline, mefloquine, primaquine, meropenem, and tafenoquine. 
     In some embodiments, survival, growth or development of the microbial cell in a sample is determined by counting the number of cells observed. In some embodiments, survival, growth or development of the microbial cell in a sample is determined by counting the number of cells observed relative to unperturbed wells. In some embodiments, survival, growth or development of the microbial cell in a sample is determined by measuring cell metabolism. In some embodiments, growth or development of the microbial cell in a sample is determined by measuring cell metabolism at varying concentrations of one or more antimicrobial agents. In some embodiments, metabolic measurements include, but are not limited to, concentration of dissolved carbon dioxide, heat dissipation, oxygen consumption, expressed genes involved in cell homeostasis, stress response, division, and/or growth, and/or cell membrane integrity, and/or cell wall integrity, and/or S-layer integrity (live/dead stain). 
     Inference of Potential Antimicrobial Resistance. 
     To enable prediction of antimicrobial resistance in the future, HiPR-FISH can be applied to not only measure the microbial identity via the rRNA sequences, but also measure the presence of antimicrobial genes, proteins, or metabolic products. To measure the presence of antimicrobial genes, panels of probes that are specific and only specific to a list of antimicrobial genes are designed. These probes are similarly encoded into binary barcodes by adding flanking sequences to the encoding sequences. These flanking sequences may be readout sequences or sequences for additional signal amplification. In the case where the flanking sequences are readout sequences, the specimen can be hybridized with readout probes and imaged on an imaging device. In the case where the flanking sequences are initiator sequences, the specimen is subjected to a round of signal amplification using amplifier probes. The amplifier probes may be conjugated with fluorophores. If the amplifier probes are already conjugated with fluorophores, the specimen can be imaged on an imaging device after amplification hybridization. If the amplifiers are not conjugated with fluorophores, the amplifier probes will contain a readout sequence. The amplified specimen is then hybridized with fluorescently labeled readout probes before being imaged on an imaging device. To measure the presence of antimicrobial proteins, antibodies conjugated with DNA readout sequences are engineered. The DNA barcoded antibodies will bind to proteins of interest, and the labeled specimen will be hybridized with fluorescently labeled readout probes before being imaged on an imaging device. To measure metabolic products such as sugars or lipids, DNA barcodes will be conjugated to molecules that bind specifically to the sugars or lipids of interest. The labeled specimen will then be hybridized with fluorescently labeled readout probes before being imaged on an imaging device. For measurement of proteins, sugars, and/or lipids, amplifier probes may also be used in a similar fashion as described for gene targets to increase signal and reduce the influence of noise. Examples of imaging devices include, but are not limited to, epifluorescent microscopes, confocal microscopes, multi-photon microscopes, and light-sheet microscopes. 
     Any number of genetic changes can affect the susceptibility of an organism to an antimicrobial agent or drug. For example, permeability changes in the bacterial cell wall can restrict antimicrobial access to target sites, changes in pumps can alter the efflux of the antimicrobial from the cell, proteins may enzymatically modify or degrade the antimicrobial agent, the cell may acquire an alternative metabolic pathway to that inhibited by the antimicrobial agent, the target of the antimicrobial agent may be modified, or the target enzyme may be overproduced. 
     In some embodiments, the present methods detect mutations that influence the development of antimicrobial resistance or susceptibility, such as nucleotide substitutions in the 23S rRNA gene that cause macrolide resistance, single nucleotide polymorphisms in ribosomal proteins such as L4 or L22, mutations within the rpsL gene, or frame shift mutation in ddl gene encoding a cytoplasm enzyme D-Ala-D-Ala ligase. 
     In some embodiments, the present methods can identify genetic changes in the microorganism compared to unmodified microorganisms of the same type. In some embodiments, the present methods identify deletions, duplications, single nucleotide polymorphisms (SNPs), frame-shift mutations, inversions, insertions, and/or substitutions associated with the development of susceptibility or resistance to a given antimicrobial agent. In some embodiments, the present methods identify mutations associated with increased drug resistance in genes including, but not limited to, genes encoding multidrug resistance proteins (e.g. PDR1, PDR3, PDR7, PDR9), ABC transporters (e.g. SNQ2, STE6, PDR5, PDR10, PDR11, YOR1), membrane associated transporters (GAS1, D4405), soluble proteins (e.g. G3PD), RNA polymerase, rpoB, gyrA, gyrB, 16S RNA, 23S rRNA, NADPH nitroreductase, sul2, strAB, tetAR, aac3-iid, aph, sph, cmy-2, floR, tetB, aadA, aac3-VIa, and sul1. 
     Microorganisms 
     In some aspects, the present disclosure provides methods for identifying and characterizing an infectious microorganism such as a virus, bacterium, parasite, or fungus. The infectious microorganism can be a microorganism that causes infections in a human or an animal such as a species of livestock, poultry, and fish. 
     In some embodiments, the list of phyla of microorganisms include phyla Actinobacteria, Aquiflcae, Armatimonadetes, Bacteroidetes, Chlamydiae, Chlorobi, Chloroflexi, Chrysiogenetes, Cyanobacteria, Chrysiogenetes, Deferribacteres,  Deinococcus - thermus , Dictyoglomi, Elusimicrobia, Fibrobacteres, Firmicutes, Fusobacteria, Gemmatimonadetes, Lentisphaerae, Nitrospirae, Planctomycetes, Proteobacteria, Spirochaetia, Synergistetes, Thermodesulfobacteria, Thermotogae, and Verrucomicrobia. 
     In some embodiments, the present disclosure provides methods for identifying and characterizing a virus including but not limited to, bacteriophage, RNA bacteriophage (e.g. MS2, AP205, PP7 and Qβ),  Helicobacter pylori , infectious haematopoietic necrosis virus (IHNV), parvovirus, Herpes Simplex Virus, Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Measles virus, Mumps virus, Rubella virus, Human Immunodeficiency Virus (HIV), Influenza virus, Rhinovirus, Rotavirus A, Rotavirus B, Rotavirus C, Respiratory Syncytial Virus (RSV), Varicella zoster, Poliovirus, Norovirus, Zika Virus, Dengue Virus, Rabies Virus, Newcastle Disease Virus, and White Spot Syndrome Virus. In some embodiments, the methods identify and characterize a cell (e.g. human cell) infected with a virus of the disclosure. 
     In some embodiments, the present disclosure provides methods for identifying and characterizing a bacterium including but not limited to,  Mycobacterium, Streptococcus, Staphylococcus, Shigella, Campylobacter, Salmonella, Clostridium, Corynebacterium, Pseudomonas, Neisseria, Listeria, Vibrio, Bordetella, E. coli  (including pathogenic  E. coli ),  Pseudomonas aeruginosa, Enterobacter cloacae, Mycobacterium tuberculosis, Staphylococcus aureus, Helicobacter pylori , and  Legionella . In some embodiments, the present disclosure provides methods for identifying and characterizing a bacterium including, but not limited to,  Acinetobacter baumannii, Citrobacter freundii, Citrobacter koseri, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus saprophyticus, Streptococcus agalactiae , or a combination thereof. 
     In some embodiments, the present disclosure provides methods for identifying and characterizing a parasite including but not limited to,  Plasmodium  (i.e.  P. falciparum, P. malariae, P. ovale, P. knowlesi , and  P. vivax ),  Trypanosoma, Toxoplasma, Giardia , and  Leishmania, Cryptosporidium , helminthic parasites:  Trichuris  spp. (whipworms),  Enterobius  spp. (pinworms),  Ascaris  spp. (roundworms),  Ancylostoma  spp. and  Necator  spp. (hookworms),  Strongyloides  spp. (threadworms),  Dracunculus  spp. (Guinea worms),  Onchocerca  spp. and  Wuchereria  spp. (filarial worms),  Taenia  spp.,  Echinococcus  spp., and  Diphyllobothrium  spp. (human and animal cestodes),  Fasciola  spp. (liver flukes) and  Schistosoma  spp. (blood flukes). 
     In some embodiments, the present disclosure provides methods for identifying and characterizing a fungus including but not limited to,  Aspergillus, Candida, Blastomyces, Coccidioides, Cryptococcus, Pneumocystis, Mucor, Rhizopus, Rhizomucor, Fusarium, Scedosporium , and  Histoplasma.    
     Kits 
     Another aspect of the disclosure is directed to kits that allow practicing the methods of the present disclosure. 
     In some embodiments, the disclosure is directed to a kit which includes a list of taxa of microorganisms, wherein each taxon is assigned a unique n-bit binary code selected from a plurality of unique n-bit binary codes, wherein n is an integer greater than 1; a set of n number of decoding probes, wherein each decoding probe corresponds to a digit in the plurality of unique n-bit binary codes, is conjugated with a label that provides a detectable signal, wherein the labels on the decoding probes are different from each other, and is substantially complementary to a readout sequence selected from a set of n number of readout sequences; and instructions on how to design a set of encoding probes, wherein the set of encoding probes includes a plurality of subsets of encoding probes, wherein each encoding probe comprises a targeting sequence and one or more readout sequences, the encoding probes within each subset comprise a targeting sequence that is specific to a taxon in the list of taxa of microorganisms and is different from a targeting sequence of the encoding probes of another subset, and the readout sequences in the encoding probes within a subset are selected from the set of n number of readout sequences based on the unique n-bit binary code assigned to the taxon which the targeting sequence of the subset is specific to. 
     In some embodiments, the encoding probes within each subset comprise at least one targeting sequence that is specific to a taxon. In some embodiments, the encoding probes within each subset comprise at least two targeting sequences that are specific to the same taxon. 
     In some embodiments, the kit includes a device to practice the methods of the present disclosure. In some embodiments, the device is a multiwell platform. In some embodiments, the multiwell platform contains between 2 and 400 well, or 2 and 384 well, or 8 and 100 well. In some embodiments, the multiwell platform contains 2 wells, 3 wells, 4 wells, 5 wells, 6 wells, 7 wells, 8 wells, 9 wells, 10 wells, 12 wells, 24 wells, 25 wells, 30 wells, 48 wells, 50 wells, 75 wells, 96 wells, 100 wells, 150 wells, 200 wells, 250 wells, 300 wells, 350 wells, 384 wells, or 400 wells. In some embodiments, the wells contain drug-inoculated or drug-free agar, agarose, polyethylene glycol, or polyacrylamide. In some embodiments, the devices are a single or a double layer of silicon. In some embodiments, a plastic flow chamber is attached for HiPR-FISH processing and readout. 
     Biological Samples 
     The methods disclosed herein can be performed directly in a biological sample, without the need to isolate and culture microorganisms. In some embodiments, the biological sample is a biological fluid or a tissue sample. In some embodiments, the biological sample includes, but is not limited to, bronchoalveolar lavage fluid (BAL), blood, serum, plasma, urine, cerebrospinal fluid, pleural fluid, synovial fluid, ocular fluid, peritoneal fluid, amniotic fluid, gastric fluid, lymph fluid, interstitial fluid, tissue homogenate, cell extracts, saliva, sputum, stool, physiological secretions, tears, mucus, sweat, milk, semen, seminal fluid, vaginal secretions, fluid from ulcers and other surface eruptions, blisters, and abscesses, and extracts of tissues associated with medical implants, and extracts of tissues including biopsies of normal, malignant, and suspect tissues or any other constituents of the body which may contain the microorganism of interest. In some embodiments, the sample is a human oral microbiome sample. In some embodiments, the sample is a whole organism. 
     In some embodiments, the sample is obtained from a patient diagnosed with, or suspected to be suffering from an infection, disease, or disorder. In some embodiments, the patient has been diagnosed with, or is suspected to be suffering from a bacterial, viral, fungal, or parasitic infection. In some embodiments, the infection includes, but is not limited to, tetanus, diphtheria, pertussis, pneumonia, meningitis, campylobacteriosis, mumps, measles, rubella, polio, flu, hepatitis, chickenpox, malaria, toxoplasmosis, giardiasis, or leishmaniasis. 
     In some embodiments, the patient has been diagnosed with, or is suspected to be suffering from an infection caused by a bacterium selected from the group consisting of:  Mycobacterium, Streptococcus, Staphylococcus, Shigella, Campylobacter, Salmonella, Clostridium, Corynebacterium, Pseudomonas, Neisseria, Listeria, Vibrio, Bordetella, Helicobacter pylori , and  Legionella.    
     In some embodiments, the patient has been diagnosed with, or is suspected to be suffering from an infection caused by a virus selected from the group consisting of: bacteriophage, RNA bacteriophage (e.g. MS2, AP205, PP7 and Qβ), Infectious Haematopoietic Necrosis Virus, Parvovirus, Herpes Simplex Virus, Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Measles virus, Mumps virus, Rubella virus, HIV, Influenza virus, Rhinovirus, Rotavirus A, Rotavirus B, Rotavirus C, Respiratory Syncytial Virus (RSV), Varicella zoster, and Poliovirus, Norovirus, Zika virus, Dengue Virus, Rabies Virus, Newcastle Disease Virus, and White Spot Syndrome Virus. 
     In some embodiments, the patient has been diagnosed with, or is suspected to be suffering from an infection caused by a parasite selected from the group consisting of:  Plasmodium, Trypanosoma, Toxoplasma, Giardia, Leishmania, Cryptosporidium , helminthic parasites:  Trichuris  spp.,  Enterobius  spp.,  Ascaris  spp.,  Ancylostoma  spp. and  Necator  spp.,  Strongyloides  spp.,  Dracunculus  spp.,  Onchocerca  spp. and  Wuchereria  spp.,  Taenia  spp.,  Echinococcus  spp., and  Diphyllobothrium  spp.,  Fasciola  spp., and  Schistosoma  spp. 
     HiPR-Swap 
     Another aspect of the disclosure is directed to a method of analyzing a sample by performing multiple imaging rounds exchanging emissive readout probes which are referred to herein as HiPR-Swap. 
     HiPR-Swap is motivated by a need to target hundreds of thousands of rRNA, mRNA, and other molecules in the microbiomes and the host tissue in order to describe host-microbiome interactions. For example, to image on average 100 unique mRNAs in roughly 1000 taxa in the gut microbiome, along with all mammalian host transcripts would require us to be able to uniquely barcode ˜150,000 targets. 
     Several FISH-based methods use multiple rounds of imaging to achieve high multiplexity in their assays. Multiple rounds can be performed by: (1) photobleaching fluorescent probes before applying a next round of fluorescent probes; (2) applying DNAse to the specimen to degrade fluorescent probes before applying a next round of fluorescent probes; (3) adding photocleavable or chemically-cleavable linker molecules to the fluorescent probes, and performing the cleavage to remove fluorescence signal before applying a next round of fluorescent probes; (4) stripping probes using washes with high (&gt;50%) formamide concentrations and/or low salt (≤2×SSC) and/or high temperatures (≥37° C.). These methods, however, are undesirable for a multitude of reasons, for example, they can be time consuming and have potential for photodamage. They can also be detrimental to sample integrity, are cost-prohibitive at scale, and possibly chemically incompatible. In addition, some can remove encoding probes necessary to conduct FISH-based methods. To overcome these deficiencies, the present disclosure uses DNA exchange as a method to quickly, specifically, carefully replace HiPR-FISH readout probes without disturbing encoding and/or amplifier probes. This method is referred to as HiPR-Swap. 
     High Phylogenetic Resolution microbiome mapping by Fluorescence in situ Hybridization (HiPR-FISH), is a versatile technology that uses binary encoding, spectral imaging, and machine learning based decoding to create micron-scale maps of the locations and identities of hundreds of microbial species in complex communities. See, for example, Shi, H. et al. “Highly multiplexed spatial mapping of microbial communities.”  Nature  vol. 588, 7839 (2020): 676-681 and PCT Patent Publication WO 2019/173555, filed Mar. 7, 2019. The contents of the aforementioned disclosures are each incorporated herein by reference in their entireties. 
     In the HiPR-Swap method, readout and encoding probes are designed such that the “landing pad” (the region on the encoding probe to which the readout probe binds) is shorter than or equal to in length to the readout probe. The landing pad being shorter than the readout probe creates a single-stranded overhang of the readout probe, as it extends past the end of the landing pad. The bigger the difference in length, the faster the exchange happens but there is also the risk of having a less stable readout probe being on the landing pad. Accordingly, there is a balance that needs to be struck to achieve a complete hybridization/exchange. In some instances, when the readout probe is of the same length as the landing pad, using a high concentration of exchange probes can result in a complete swap. 
     After a readout probe is bound, an exchange probe can be added to the specimen. The exchange probe can be constructed to be of equal length and a perfect reverse complement to the readout probe. In some instances, the exchange probe may contain locked nucleic acids to increase the stability of the exchange-readout pair. When added, the exchange probe seeds a hybridization to the exposed area of the readout probe. Over a short period of time the exchange probe completely hybridizes to the readout probe, thereby removing it from the encoding probe where it can be washed away. Importantly, orthogonal readout and exchange probes can be added simultaneously to reduce assay time. 
     Accordingly, a method for analyzing a sample can include:
         contacting at least one encoding probe with the sample to produce a first complex, wherein each encoding probe comprises a targeting sequence, a first landing pad sequence, and a second landing pad sequence;   adding at least one first emissive readout probe to the first complex, wherein the first emissive readout probe comprises a label and a sequence complementary to the first landing pad sequence;   acquiring one or more emission spectra from the first emissive readout probe;   adding an exchange probe to the sample, wherein the exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequence,   hybridizing the exchange probe to the first emissive readout probe to form a second complex;   removing the second complex from the sample,   adding at least one second emissive readout probe to the first complex, wherein the second emissive readout probe comprises a label and a sequence complementary to the second landing pad sequence;   acquiring one or more emission spectra from the second emissive readout probe;   repeating the aforementioned steps for at least one different encoding probe;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a species of interest; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     In some embodiments, more than one type of probe set (e.g., encoding probe, emissive readout probes, and exchange probes) may be introduced to a sample. For example, there may be from at least 2 to at least 1 billion distinguishable probe sets that are introduced to a sample. In some embodiments, at least 2, at least 5, at least 10, at least 25, at least 50, at least 75, at least 100, at least 300, at least 1,000, at least 3,000, at least 10,000, at least 30,000, at least 100,000, at least 500,000, or at least 1,000,000, at least 10,000,000, at least 50,000,000, at least 100,000,000, at least 500,000,000, or at least 1,000,000,000 distinguishable probe sets that are introduced to a sample. In some embodiments, the distinct probes are introduced simultaneously. In some embodiments, the distinct probes are introduced sequentially. In some embodiments, more than one type of probe set may be introduced to a sample over multiple rounds, with each round having multiple probe pools. 
     Encoding Probe Hybridization 
     In the methods described herein for analyzing a sample, the method can include contacting at least one encoding probe with the sample to produce a first complex, wherein each encoding probe includes a targeting sequence, a first landing pad sequence, and a second landing pad sequence. This step may also be referred to as the “encoding probe hybridization” step. In here, at least one encoding probe is contacted with the sample to produce a first complex. The first complex can include the targeting sequence of the encoding probe hybridized to the nucleic acid target sequence. 
     In some embodiments, contacting the encoding probes with the sample is contacting the encoding probes with at least one nucleotide sequence of the sample. In some embodiments, contacting the encoding probes with the sample is hybridizing the encoding probe (e.g., via the targeting sequence present in the encoding probe) with a target sequence present in the sample. 
     In some embodiments, in order to contact encoding probes with the sample, the sample can be digested or lysed so as to allow the encoding probes (and other probes described herein) to contact with the target sequence. 
     In some embodiments, to contact the at least one encoding probe with the sample to produce a first complex, encoding buffer is added to the sample. In some embodiments, a pre-hybridization step can be performed prior to adding the encoding probe. In some embodiments, the encoding buffer can be added to the sample without the encoding probe. In some embodiments, the encoding buffer can be added to the sample about 30 minutes prior to adding the encoding probe. 
     In some embodiments, the encoding buffer can include a denaturing/deionizing agent, a salt buffer, a detergent, a polyanionic polymer, a blocking agent, acids, or combinations thereof. In some embodiments, the encoding buffer can include more than one type of agent, for example, the encoding buffer can include two or more polyanionic polymers and/or two or more blocking agents. In some embodiments, the encoding buffer can include a denaturing/deionizing agent, a salt buffer, a detergent, two polyanionic polymers, two blocking agents, and an acid. 
     In some embodiments, the encoding buffer can include a denaturing/deionizing agent. In some embodiments, the denaturing/deionizing agent can be formamide, ethylene carbonate, or urea. In some embodiments, the encoding buffer can include about 10% (v/v) to about 50% (v/v), about 15% (v/v) to about 45% (v/v), about 20% (v/v) to about 40% (v/v), about 25% (v/v) to about 35% (v/v), about 10% (v/v), 15% (v/v), 20% (v/v), 25% (v/v), or 30% (v/v) of a denaturing/deionizing agent (e.g., ethylene carbonate). 
     In some embodiments, the encoding buffer can include a salt buffer. In some embodiments, the salt buffer is saline sodium citrate (SSC), NaCl, or MgCl 2 . In some embodiments, the encoding buffer can include about 2× to about 20×, about 5× to about 10×, or about 5× of a salt buffer (e.g., saline sodium citrate (SSC)). 
     In some embodiments, the encoding buffer can include at least one polyanionic polymer. In some embodiments, the encoding buffer can include one polyanionic polymer. In some embodiments, the encoding buffer can include two polyanionic polymers. In some embodiments, the polyanionic polymer can be dextran sulfate, heparin, or polyglutamic acid. In some embodiments, the encoding buffer can include about 2.5% (v/v) to about 25% (v/v), about 5% (v/v) to about 15% (v/v), about 7.5% (v/v) to about 12.5% (v/v), about 5% (v/v), or about 10% (v/v) of a polyanionic polymer (e.g., dextran sulfate). In some embodiments, the encoding buffer can include about 20 μg/mL to about 80 μg/mL, about 30 μg/mL to about 70 μg/mL, about 40 μg/mL to about 60 μg/mL, or about 50 μg/mL of a polyanionic polymer (e.g., heparin). 
     In some embodiments, the encoding buffer can include a detergent. In some embodiments, the detergent can be Tween 20, Tween 80, sodium dodecyl sulfate (SDS), Triton X-100, Triton X-114, NP-40, Brij-35, Brij-58. N-Dodecyl-beta-maltoside, Octyl-beta-glucoside, octylthioglucoside (OTG). In some embodiments, the encoding buffer can include about 0.01% (v/v) to about 1.0% (v/v), about 0.05% (v/v) to about 0.5% (v/v), or about 0.1% (v/v), or about 0.05% (v/v) of detergent (e.g., SDS). 
     In some embodiments, the encoding buffer can include an acid. In these embodiments, the acid lowers the pH of the buffer. In some embodiments, the acid can be citric acid. In some embodiments, the encoding buffer can include about 1 mM to about 30 mM, about 5 mM to about 15 mM, about 5 mM to about 10 mM, about 7 mM to about 10 mM, or about 9 mM of an acid (e.g., citric acid). 
     In some embodiments, the encoding buffer can include at least one blocking agent. In some embodiments, the encoding buffer can include one blocking agent. In some embodiments, the blocking agents can be Denhardt&#39;s solution, bovine serum albumin (BSA), salmon sperm DNA, Ficoll, polyvinyl pyrrolidone (PVP),  E. coli  tRNA, casein solution, or random hexamers. In some embodiments, the encoding buffer can include about 0.1× to about 10×, about 0.5× to about 5×, about 1× to about 2×, or about 1× of a blocking agent (e.g., Denhardt&#39;s solution). 
     In some embodiments, the encoding buffer can include ethylene carbonate, dextran sulfate, SSC, Denhardt&#39;s solution, and SDS. In some embodiments, the encoding buffer can include 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, and 0.01% SDS. 
     First Emissive Readout Probe Hybridization 
     Following the hybridization of the encoding probe with the target sequence to form a first complex, at least one first emissive readout probe is added to the first complex, wherein the first emissive readout probe comprises a label and a sequence complementary to the first landing pad sequence. In some embodiments, this step may be referred to as the “readout probe hybridization” step. In here, the emissive readout probes hybridize to their complementary sequences present in the first complex (e.g., first landing pad sequence). 
     In some embodiments, the encoding probe and the readout probe hybridization occur in the same step. In some embodiments, the readout probe hybridization is performed in the presence of the encoding buffer described above. In some embodiments, the encoding probe hybridization step, the readout probe hybridization step, and the readout step can occur sequentially or substantially in the same step. 
     In some embodiments, to hybridize the readout probes to the first complex, readout buffer is added to the sample. In some embodiments, to image the readout probes, a wash buffer is added to the sample. 
     In some embodiments, the wash buffer can include a salt buffer, a pH stabilizer, and a chelating agent. 
     In some embodiments, the readout probes are added so they achieve a final concentration of about 10 nM to about 20 μM, or about 10 nM to about 10 μM, or about 100 nM to about 1 μM, about 200 nM to about 500 nM, or about 200 nM, about 300 nM, about 400 nM, or about 500 nM for each readout probe. In some embodiments, the readout probes are added so they achieve a final concentration of about 400 nM. 
     In some embodiments, the wash buffer can include a salt buffer. In some embodiments, the salt buffer is saline sodium citrate (SSC), NaCl, or MgCl 2 . In some embodiments, the wash buffer can include about 2× to about 20×, about 5× to about 10×, or about 5× of a salt buffer (e.g., saline sodium citrate (SSC)). In some embodiments, the wash buffer can include about 50 mM to about 500 mM, or about 100 mM to about 300 mM, or about 150 mM to about 250 mM, or about 215 mM or salt buffer (e.g., NaCl). 
     In some embodiments, the wash buffer can include a pH stabilizer. In some embodiments, the pH stabilizer can be at least one of tris-HCl, citric acid, SSC, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sucrose/EDTA/Tris-HCl (SET), potassium phosphate, tris(hydroxymethyl)methylamino]propanesulfonic acid (TAPS), NaOH, 3-(N-morpholino)propanesulfonic acid (MOPS), Tricine, Bicine, sodium pyrophosphate, piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES), SSPE. In some embodiments, the pH stabilizer can be tris-HCl. In some embodiments, the wash buffer can include about 5 mM to about 30 mM, about 10 mM to about 20 mM, about 10 mM, or about 20 mM of a pH stabilizer (e.g., tris-HCl). 
     In some embodiments, the wash buffer can include a chelating agent. In some embodiments, the chelating agent is at least one of EDTA, Ethylene glycol tetraacetic acid (EGTA), Salicylic acid, Triethanolamine (TEA), or Dimercaptopropanol. In some embodiments, the chelating agent is EDTA. In some embodiments, the wash buffer can include about 1 mM to about 10 mM, about 2 mM to about 5 mM, or about 5 mM of a chelating agent (e.g., EDTA). 
     In some embodiments, the wash buffer can include NaCl, tris-HCl, and EDTA. In some embodiments, the wash buffer can include 215 mM NaCl, 20 mM tris-HCl, and 5 mM EDTA. 
     Exchange Probe Hybridization 
     After acquiring one or more emission spectra from the first emissive readout probe, an exchange probe is added so it removes the first emissive readout probe from the complex so it allows for another emissive readout probe and imaging step to occur. In some embodiments, the addition of the exchange probe and addition of the second emissive readout probe occur in the same step. In some embodiments, the addition of the exchange probe and addition of the second emissive readout probe occur sequentially. 
     In some embodiments, the exchange probes are added so they achieve a final concentration of about 10 nM to about 20 or about 10 nM to about 10 or about 100 nM to about 1 about 200 nM to about 500 nM, or about 200 nM, about 300 nM, about 400 nM, or about 500 nM for each exchange probe. In some embodiments, the exchange probes are added so they achieve a final concentration of about 400 nM. 
     In some embodiments, to contact the exchange probe with the first emissive readout probe to produce a second complex, exchange buffer is added to the sample. In some embodiments, the exchange buffer can include a denaturing/deionizing agent, a salt buffer, a detergent, a polyanionic polymer, a blocking agent, acids, or combinations thereof. In some embodiments, the exchange buffer can include more than one type of agent, for example, the encoding buffer can include two or more polyanionic polymers and/or two or more blocking agents. In some embodiments, the exchange buffer can include a denaturing/deionizing agent, a salt buffer, a detergent, two polyanionic polymers, two blocking agents, and an acid. 
     In some embodiments, the exchange buffer can include a denaturing/deionizing agent. In some embodiments, the denaturing/deionizing agent can be formamide, ethylene carbonate, or urea. In some embodiments, the exchange buffer can include about 10% (v/v) to about 50% (v/v), about 15% (v/v) to about 45% (v/v), about 20% (v/v) to about 40% (v/v), about 25% (v/v) to about 35% (v/v), about 10% (v/v), 15% (v/v), 20% (v/v), 25% (v/v), or 30% (v/v) of a denaturing/deionizing agent (e.g., ethylene carbonate). 
     In some embodiments, the exchange buffer can include a salt buffer. In some embodiments, the salt buffer is saline sodium citrate (SSC), NaCl, or MgCl 2 . In some embodiments, the exchange buffer can include about 2× to about 20×, about 5× to about 10×, or about 5× of a salt buffer (e.g., saline sodium citrate (SSC)). 
     In some embodiments, the exchange buffer can include at least one polyanionic polymer. In some embodiments, the exchange buffer can include one polyanionic polymer. In some embodiments, the exchange buffer can include two polyanionic polymers. In some embodiments, the polyanionic polymer can be dextran sulfate, heparin, or polyglutamic acid. In some embodiments, the exchange buffer can include about 2.5% (v/v) to about 25% (v/v), about 5% (v/v) to about 15% (v/v), about 7.5% (v/v) to about 12.5% (v/v), about 5% (v/v), or about 10% (v/v) of a polyanionic polymer (e.g., dextran sulfate). In some embodiments, the exchange buffer can include about 20 μg/mL to about 80 μg/mL, about 30 μg/mL to about 70 μg/mL, about 40 μg/mL to about 60 μg/mL, or about 50 μg/mL of a polyanionic polymer (e.g., heparin). 
     In some embodiments, the exchange buffer can include a detergent. In some embodiments, the detergent can be Tween 20, Tween 80, sodium dodecyl sulfate (SDS), Triton X-100, Triton X-114, NP-40, Brij-35, Brij-58. N-Dodecyl-beta-maltoside, Octyl-beta-glucoside, octylthioglucoside (OTG). In some embodiments, the exchange buffer can include about 0.01% (v/v) to about 1.0% (v/v), about 0.05% (v/v) to about 0.5% (v/v), or about 0.1% (v/v), or about 0.05% (v/v) of detergent (e.g., SDS). 
     In some embodiments, the exchange buffer can include an acid. In these embodiments, the acid lowers the pH of the buffer. In some embodiments, the acid can be citric acid. In some embodiments, the exchange buffer can include about 1 mM to about 30 mM, about 5 mM to about 15 mM, about 5 mM to about 10 mM, about 7 mM to about 10 mM, or about 9 mM of an acid (e.g., citric acid). 
     In some embodiments, the exchange buffer can include at least one blocking agent. In some embodiments, the exchange buffer can include one blocking agent. In some embodiments, the blocking agents can be Denhardt&#39;s solution, bovine serum albumin (BSA), salmon sperm DNA, Ficoll, polyvinyl pyrrolidone (PVP),  E. coli  tRNA, casein solution, or random hexamers. In some embodiments, the exchange buffer can include about 0.1× to about 10×, about 0.5× to about 5×, about 1× to about 2×, or about 1× of a blocking agent (e.g., Denhardt&#39;s solution). 
     In some embodiments, the exchange buffer can include ethylene carbonate, dextran sulfate, SSC, Denhardt&#39;s solution, and SDS. In some embodiments, the exchange buffer can include 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, and 0.01% SDS. 
     Second Emissive Readout Probe Hybridization 
     Following the hybridization of the exchange probe to the first emissive readout probe, a second emissive readout probe is added. In some embodiments, this step may be referred to as the “second readout probe hybridization” step. In here, the second emissive readout probe hybridizes to its complementary sequences present in the first complex (e.g., second landing pad sequence). 
     In some embodiments, the second emissive readout probe hybridization is performed in the presence of the encoding buffer described above. In some embodiments, to image the second readout probes, a wash buffer is added to the sample. In some embodiments, the wash buffer is the wash buffer described above. 
     In some embodiments, the second emissive readout probes are added so they achieve a final concentration of about 10 nM to about 10 or about 100 nM to about 1 about 200 nM to about 500 nM, or about 200 nM, about 300 nM, about 400 nM, or about 500 nM for each readout probe. In some embodiments, the second emissive readout probes are added so they achieve a final concentration of about 400 nM. 
     In some embodiments, adding an exchange probe to the sample, hybridizing the exchange probe to the first emissive readout probe, and removing the second complex from the sample are performed in the same step. In some embodiments, adding an exchange probe to the sample, hybridizing the exchange probe to the first emissive readout probe, and removing the second complex from the sample are performed sequentially. In some embodiments, adding an exchange probe to the sample, hybridizing the exchange probe to the first emissive readout probe, removing the second complex from the sample, and adding the second emissive readout probe are performed in the same step. In some embodiments, adding an exchange probe to the sample, hybridizing the exchange probe to the first emissive readout probe, removing the second complex from the sample, and adding the second emissive readout probe are performed sequentially. 
     In some embodiments, hybridizing the exchange probe to the first or second emissive readout probe results in de-hybridization of the first or second emissive readout probe from the first or second landing pad sequence. In some embodiments, the step is achieved from about 30 seconds to about 1 hour. In some embodiments, the step is achieved within 30 seconds, 1 minute, 5 minutes, 10 minutes, 12 minutes, 15 minutes, 30 minutes, 45 minutes, or 1 hour. In some embodiments, the step is achieved within 1 hour. In some embodiments, the step is achieved overnight. 
     In another aspect, a method for analyzing a sample can include:
         generating a set of probes, wherein each probe comprises:   (i) a targeting sequence;   (ii) a first landing pad sequence; and   (iii) a second landing pad sequence;   contacting the set of probes with the sample to permit hybridization of the probes to nucleotides present in the sample to produce a complex;   adding a first set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   acquiring one or more emission spectra from the first emissive readout probe;   adding a set of exchange probes to the sample, wherein each exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequences,   hybridizing the exchange probes to the first emissive readout probes to form a second complex;   removing the second complex from the sample,   adding a second set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   acquiring one or more emission spectra from the second emissive readout probe;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a species of interest; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     Sample 
     In some embodiments, the sample is at least one of a cell, a cell suspension, a tissue biopsy, a tissue specimen, urine, stool, blood, serum, plasma, bone biopsies, bone marrow, respiratory specimens, sputum, induced sputum, tracheal aspirates, bronchoalveolar lavage fluid, sweat, saliva, tears, ocular fluid, cerebral spinal fluid, pericardial fluid, pleural fluid, peritoneal fluid, placenta, amnion, pus, nasal swabs, nasopharyngeal swabs, oropharyngeal swabs, ocular swabs, skin swabs, wound swabs, mucosal swabs, buccal swabs, vaginal swabs, vulvar swabs, nails, nail scrapings, hair follicles, corneal scrapings, gavage fluids, gargle fluids, abscess fluids, wastewater, or plant biopsies. 
     In some embodiments, the sample is a cell. In some embodiments, the cell is a bacterial cell. In some embodiments, the cell is a eukaryotic cell. In some embodiments the eukaryotic cell is a unicellular organism including protozoa, chromista, algae, or fungi. In some embodiments the eukaryotic cell is part of a multicellular organism from chromista, plantae, fungi, or animalia. In some embodiments the sample is a tissue composed of cells. In some embodiments the cell contains foreign DNA/RNA from viruses, plasmids, and bacteria. 
     In some embodiments, the sample can include a plurality of cells. In some embodiments, each cell in the plurality of cells can include a specific targeting sequence, which may or may not be the same from the other targeting sequences. 
     In some embodiments, the sample is a human oral microbiome sample. In some embodiments, the sample is a whole organism. 
     In some embodiments, the sample is obtained from a patient diagnosed with, or suspected to be suffering from an infection, disease, or disorder. In some embodiments, the patient has been diagnosed with, or is suspected to be suffering from a bacterial, viral, fungal, or parasitic infection. In some embodiments, the infection includes, but is not limited to, Acute Flaccid Myelitis, Anaplasmosis, Anthrax, Babesiosis, Botulism, Brucellosis, Campylobacteriosis, Carbapenem-resistant Infection (CRE/CRPA), Chancroid, Chickenpox, Chikungunya Virus Infection (Chikungunya),  Chlamydia , Ciguatera (Harmful Algae Blooms (HABs)),  Clostridium Difficile  Infection,  Clostridium Perfringens  (Epsilon Toxin), Coccidioidomycosis fungal infection (Valley fever), COVID-19 (Coronavirus Disease 2019), Creutzfeldt-Jacob Disease, transmissible spongiform encephalopathy (CJD), Cryptosporidiosis (Crypto), Cyclosporiasis, Dengue, 1, 2, 3, 4 (Dengue Fever), Diphtheria,  E. coli  infection, Shiga toxin-producing (STEC), Eastern Equine Encephalitis (EEE), Ebola Hemorrhagic Fever (Ebola), Ehrlichiosis, Encephalitis, Arboviral or parainfectious, Enterovirus Infection, D68 (EV-D68), Enterovirus Infection, Non-Polio (Non-Polio Enterovirus), Giardiasis (Giardia), Glanders, Gonococcal Infection (Gonorrhea), Granuloma inguinale,  Haemophilus  Influenza disease, Type B (Hib or H-flu), Hantavirus Pulmonary Syndrome (HPS), Hemolytic Uremic Syndrome (HUS), Hepatitis (A, B, C, D, and/or E), Herpes Herpes Zoster, zoster VZV (Shingles), Histoplasmosis infection (Histoplasmosis), Human Immunodeficiency Virus/AIDS (HIV/AIDS), Human Papillomavirus (HPV), Influenza (Flu), Lead Poisoning, Legionellosis (Legionnaires Disease), Leishmaniasis, Leprosy (Hansens Disease), Leptospirosis, Listeriosis (Listeria), Lyme Disease, Lymphogranuloma venereum infection (LGV), Malaria, Measles, Melioidosis, Meningitis, Viral (Meningitis, viral), Meningococcal Disease, Bacterial (Meningitis, bacterial), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), Mononucleosis, Multisystem Inflammatory Syndrome in Children (MIS-C), Mumps, Norovirus, Paralytic Shellfish Poisoning (Paralytic Shellfish Poisoning, Ciguatera), Pediculosis (Lice, Head and Body Lice), Pelvic Inflammatory Disease (PID), Pertussis (Whooping Cough), Plague; Bubonic, Septicemic, Pneumonic (Plague), Pneumococcal Disease (Pneumonia), Poliomyelitis (Polio), Powassan, Psittacosis (Parrot Fever), Phthiriasis (Crabs; Pubic Lice Infestation), Pustular Rash diseases (Small pox, monkeypox, cowpox), Q-Fever, Rabies, Ricin Poisoning, Rickettsiosis (Rocky Mountain Spotted Fever), Rubella,  Salmonellosis  gastroenteritis ( Salmonella ), Scabies Infestation (Scabies), Scombroid, Septic Shock (Sepsis), Severe Acute Respiratory Syndrome (SARS), Shigellosis gastroenteritis ( Shigella ), Smallpox, Staphylococcal Infection, Methicillin-resistant (MRSA), Staphylococcal Food Poisoning, Enterotoxin-B Poisoning (Staph Food Poisoning), Staphylococcal Infection, Vancomycin Intermediate (VISA), Staphylococcal Infection, Vancomycin Resistant (VRSA), Streptococcal Disease, Group A (invasive) (Strep A (invasive)), Streptococcal Disease, Group B (Strep-B), Streptococcal Toxic-Shock Syndrome, STSS, Toxic Shock (STSS, TSS), Syphilis, primary, secondary, early latent, late latent, congenital, Tetanus, Toxoplasmosis, Trichomoniasis ( Trichomonas  infection), Trichinosis Infection (Trichinosis), Tuberculosis (Latent) (LTBI), Tuberculosis (TB), Tularemia (Rabbit fever), Typhus, Typhoid Fever, Group D, Vaginosis, bacterial (Yeast Infection), Vaping-Associated Lung Injury (e-Cigarette Associated Lung Injury), Varicella (Chickenpox),  Vibrio cholerae  (Cholera), Vibriosis ( Vibrio ), Viral Hemorrhagic Fever (Ebola, Lassa, Marburg), West Nile Virus, Yellow Fever, Yersenia ( Yersinia ), or Zika Virus Infection (Zika). 
     In some embodiments, when the sample is obtained from a patient, the patient has been diagnosed with, or is suspected to be suffering from an infection caused by a bacterium selected from the group consisting of:  Acinetobacter, Actinomyces, Aerococcus, Bacteroides, Bartonella, Brucella, Bordetella, Burkholderia, Campylobacter, Chlamydia, Citrobacter, Clostridium, Corynebacterium, Edwardsiella, Elizabethkingia, Enterobacter, Enterococcus, Escherichia, Fusobacterium, Haemophilus, Helicobacter, Klebsiella, Legionella, Leptospira, Listeria, Morganella, Mycobacterium, Mycoplasma, Neisseria, Pantoea, Prevotella, Proteus, Providencia, Pseudomonas, Raoultella, Salmonella, Serratia, Shigella, Staphylococcus, Stenotrophomonas, Streptococcus, Ureaplasma , and  Vibrio.    
     In some embodiments, when the sample is obtained from a patient, the patient has been diagnosed with, or is suspected to be suffering from an infection caused by a virus selected from the group consisting of: bacteriophage, RNA bacteriophage (e.g., MS2, AP205, PP7 and Qβ), Infectious Haematopoietic Necrosis Virus, Parvovirus, Herpes Simplex Virus, Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Measles virus, Mumps virus, Rubella virus, HIV, Influenza virus, Rhinovirus, Rotavirus A, Rotavirus B, Rotavirus C, Respiratory Syncytial Virus (RSV), Varicella zoster, and Poliovirus, Norovirus, Zika virus, Dengue Virus, Rabies Virus, Newcastle Disease Virus, and White Spot Syndrome Virus. 
     In some embodiments, when the sample is obtained from a patient, the patient has been diagnosed with, or is suspected to be suffering from an infection caused by a parasite selected from the group consisting of:  Plasmodium, Trypanosoma, Toxoplasma, Giardia, Leishmania, Cryptosporidium , helminthic parasites:  Trichuris  spp.,  Enterobius  spp.,  Ascaris  spp.,  Ancylostoma  spp. and  Necator  spp.,  Strongyloides  spp.,  Dracunculus  spp.,  Onchocerca  spp. and  Wuchereria  spp.,  Taenia  spp.,  Echinococcus  spp., and  Diphyllobothrium  spp.,  Fasciola  spp., and  Schistosoma  spp. 
     Encoding Probes 
     Encoding probes are probes that bind directly to a target or targeting sequence and contain either 1 or 2 branches extending away from the hybridization site. The branches can either correspond to the readout sequences or first or second landing pad sequences. Encoding probes, for example, are designed to target bacterial ribosomal RNA (rRNA) and messenger RNA (mRNA) targets. 
     For example, rRNA-probes can contain (5′ to 3′): 
     a. Primer sequences to enrich probe pool. 
     b. A first landing pad sequence. 
     c. rRNA target complementary sequence. 
     d. A second landing pad sequence (different than b). 
     e. Primer sequences to enrich probe pool. 
     mRNA-probes contain (5′ to 3′): 
     a. Primer sequences to enrich probe pool. 
     b. A first landing pad sequence. 
     c. mRNA target complementary sequence. 
     d. A second landing pad sequence (different than b). 
     e. Primer sequences to enrich probe pool. 
     In some embodiments, each encoding probe can include a targeting sequence, a first landing pad sequence and a second landing pad sequence. 
     Primer Sequences 
     In some embodiments, the primer sequence can include about 10 to about 30, about 15 to about 25, about 18 to about 23, about 15, 16, 17, 18, 19, 20, 21, 22, or 23 nucleotides long. 
     Targeting Sequence 
     In some embodiments, the targeting sequence targets at least one of messenger RNA (mRNA), micro RNA (miRNA), long non coding RNA (lncRNA), ribosomal RNA (rRNA), small interfering RNA (siRNA), transfer RNA (tRNA), Crispr RNA (crRNA), trans-activating cirspr RNA (tracrRNA), mitochondria RNA, Intronic RNA, viral mRNA, viral genomic RNA, environmental RNA, double-stranded RNA (dsRNA), small nuclear RNA (snRNA), small nucleolar (snoRNA), piwi-interacting RNA (piRNA), genomic DNA, synthetic DNA, DNA, plasmid DNA, a plasmid, viral DNA, retroviral DNA, environmental DNA, extracellular DNA, a protein, a small molecule, or an antigenic target. In some embodiments, the target is mRNA. In some embodiments, the target is rRNA. In some embodiments, the target is mRNA and rRNA. 
     In some embodiments, the targeting sequence of the encoding probe is substantially complementary to a specific target sequence. By “substantially complementary” it is meant that the nucleic acid fragment is capable of hybridizing to at least one nucleic acid strand or duplex even if less than all nucleobases do not base pair with a counterpart nucleobase. In some embodiments, a “substantially complementary” nucleic add contains at least one sequence in which about 70%, about 71%, about 72%, about 73%, about 74%, about 75%, about 76%, about 77%, about 77%, 8%, about 79%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, to about 100%, and any range therein, of the nucleobase sequence is capable of basepairing with at least one single or double stranded nucleic acid molecule during hybridization. 
     In some embodiments, the targeting sequence is designed to have a predicted melting temperature of between about 55° C. and about 65° C. In some embodiments, the predicted melting temperature of the targeting sequence is 55° C., 56° C., 57° C., 58° C., 59° C., 60° C., 61° C., 62° C., 63° C., 64° C. or 65° C. In some embodiments, the targeting sequence can have a GC content of about 55%, 60%, 65% or 70%. 
     In some embodiments, the targeting sequence can include about 10 to about 35, about 15 to about 30, about 18 to about 30, about 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 nucleotides long. 
     In some embodiments, the targeting sequence of an encoding probe is designed using publicly-available sequence data. In some embodiments, the targeting sequence of an encoding probe design is designed using custom catalogues of the target/sample. In some embodiments, the targeting sequence of an encoding probe is designed using a database that is relevant for a system. In a specific embodiment, the system is the gut microbiome. In some embodiments, the targeting sequence of an encoding probe is designed using a database that is relevant for a disease or infection. 
     Landing Pad Sequences 
     In some embodiments, the encoding probe can include a first landing pad sequence on the 5′ end and a second landing pad sequence on the 3′ end. In some embodiments, the first and second landing pad sequences have the same sequence. 
     In some embodiments, each landing pad sequence is about 10 to about 50, about 15 to about 50, about 15 to about 40, about 10 to about 30, about 15 to about 25, about 18 to about 23, about 15, 16, 17, 18, 19, 20, 21, 22, or 23 nucleotides long. In some embodiments, each landing pad sequence is substantially complementary to the first and/or second emissive readout sequences. 
     The encoding probes, and other probes described herein, may be introduced into the sample (e.g., cell) using any suitable method. In some cases, the sample may be sufficiently permeabilized such that the probes may be introduced into the sample by flowing a fluid containing the probes around the sample (e.g., cells). In some cases, the samples (e.g., cells) may be sufficiently permeabilized as part of a fixation process. In some embodiments, samples (e.g., cells) may be permeabilized by exposure to certain chemicals such as ethanol, methanol, Triton, or the like. In some embodiments, techniques such as electroporation or microinjection may be used to introduce the probes into a sample (e.g., cell). 
     Emissive Readout Probes 
     Emissive readouts probes are oligonucleotides bound with one of ten fluorescent dyes at the 5′- and/or 3′-end. In some embodiments, each emissive readout probe comprises a label and a sequence complementary to the first landing pad sequence. 
     In some embodiments, each emissive readout probe sequence is of the same length as the first or second landing pad sequence. In some embodiments, the emissive readout probe sequence is 0 nucleotides longer than the corresponding landing pad sequence. 
     In some embodiments, each emissive readout probe sequence is from at least 1 to at least 35 nucleotides longer than the corresponding landing pad sequence. In some embodiments, each emissive readout probe sequence is at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, or 35 nucleotides longer than the corresponding landing pad sequence. In some embodiments, each emissive readout probe sequence is at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides longer than the corresponding landing pad sequence. In some embodiments, each emissive readout probe sequence is at least 5 nucleotides longer than the corresponding landing pad sequence. 
     Readout probes can be designed as follows: 
     a. Are coupled to 1, 2, or more fluorescent dyes. 
     b. Are orthogonal to all biological sequences. 
     c. Are orthogonal to each other/each other&#39;s complementary sequences. 
     In some embodiments, the readout sequence is about 10 to about 50, about 15 to about 50, about 15 to about 45, about 15 to about 35, about 15 to about 30, about 18 to about 24, about 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 nucleotides long. 
     In some embodiments, the emissive readout probe can include a label on the 5′ or 3′ end. In some embodiments, the emissive readout probe can include a label on the 5′ end and a label on the 3′ end. In some embodiments, the labels are the same. In some embodiments, the labels are different. 
     In some embodiments, the label is a fluorescent entity (fluorophore) or phosphorescent entity. In some embodiments, the label is a cyanine dye (e.g., Cy2, Cy3, Cy3B, Cy5, Cy5.5, Cy7, etc.), Alexa Fluor dye, Atto dye, photo switchable dye, photoactivatable dye, fluorescent dye, metal nanoparticle, semiconductor nanoparticle or “quantum dots”, fluorescent protein such as GFP (Green Fluorescent Protein), or photoactivatable fluorescent protein, such as PAGFP, PSCFP, PSCFP2, Dendra, Dendra2, EosFP, tdEos, mEos2, mEos3, PAmCherry, PAtagRFP, mMaple, mMaple2, and mMaple3. 
     In some embodiments, the label is Alexa Fluor 350, Alexa Fluor 405, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 514, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 561, Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 610, Alexa Fluor 633, Alexa Fluor 635, Alexa Fluor 647, Alexa Fluor 647-R-phycoerythrin, Alexa Fluor 660, Alexa Fluor 680, Alexa Fluor 680-allophycocyanin, Alexa Fluor 700, Alexa Fluor 750, Alexa Fluor 790, Alexa Fluor Plus 405, Alexa Fluor Plus 488, Alexa Fluor Plus 555, Alexa Fluor Plus 594, Alexa Fluor Plus 647, Alexa Fluor Plus 680, Alexa Fluor Plus 750, Alexa Fluor Plus 800, Pacific Blue, Pacific Green, Rhodamine Red X, DyLight 485-LS, DyLight-510-LS, DyLight 515-LS, DyLight 521-LS, Hydroxycoumarin, methoxycoumarin, Cy2, FAM, Fluorescein FITC, R-phycoerythrin (PE), Tamara, Cy3.5 581, ROX (carboxy-X-rhodamine), Red 613, Texas Red, Cy5, Cy5.5, Cy7, Allophycocyanin, ATTO 430LS, ATTO 490LS, ATTO 390, ATTO 425, Cyan 500 NHS-Ester, ATTO 465, ATTO 488, ATTO 495, ATTO Rho110, ATTO 514, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 542, ATTO 550, ATTO 565, ATTO Rho3B, ATTO Rho11, ATTO Rho12, ATTO Thio12, ATTO Rho101, ATTO 590, ATTO 594, ATTO Rho13, ATTO 610, ATTO 620, ATTO Rho14, ATTO 633, ATTO 643, ATTO 647, ATTO 647N, ATTO 655, ATTO Oxa12, ATTO 665, ATTO 680, ATTO 700, ATTO 725, ATTO 740. 
     In some embodiments, the label is imaged using widefield microscopy, point scanning confocal microscopy, spinning disk confocal microscopy, lattice lightsheet microscopy, or light field microscopy. 
     In some embodiments, the detection strategy used is channel, spectral, channel and fluorescence lifetime, or spectral and fluorescence lifetime. 
     In some embodiments, the labels used in the present methods are imaged using a microscope. In some embodiments, the microscope is a confocal microscope. In some embodiments, the microscope is a fluorescence microscope. In some embodiments, the microscope is a light-sheet microscope. In some embodiments, the microscope is a super-resolution microscope. 
     In some embodiments, the sample is on an analyzing platform, wherein the analyzing platform is a microscope slide, at least one chamber, at least one microfluidic device, at least one well, at least one plate, or at least one filter membrane. 
     Exchange Probes 
     Exchange probes are each about 10-50 or 15-50 nucleotide-long oligonucleotides. In some embodiments, each exchange probe comprises a 100% complementary sequence to a respective emissive readout probe sequence. 
     In some embodiments, the exchange sequence is about 10 to about 50, about 15 to about 50, about 15 to about 45, about 15 to about 35, about 15 to about 30, about 18 to about 24, about 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 nucleotides long. 
     In some embodiments, the encoding probes contain locked nucleic acids to stabilize the exchange reaction. 
     In some embodiments, adding an exchange probe to the sample, hybridizing the exchange probe to the first emissive readout probe, and removing the second complex from the sample are performed in the same step. In some embodiments, adding an exchange probe to the sample, hybridizing the exchange probe to the first emissive readout probe, and removing the second complex from the sample are performed sequentially. In some embodiments, adding an exchange probe to the sample, hybridizing the exchange probe to the first emissive readout probe, removing the second complex from the sample, and adding the second emissive readout probe are performed in the same step. In some embodiments, adding an exchange probe to the sample, hybridizing the exchange probe to the first emissive readout probe, removing the second complex from the sample, and adding the second emissive readout probe are performed sequentially. 
     In some embodiments, hybridizing the exchange probe to the first or second emissive readout probe results in de-hybridization of the first or second emissive readout probe from the first or second landing pad sequence. In some embodiments, the step is achieved from about 30 seconds to about 1 hour. In some embodiments, the step is achieved within 30 seconds, 1 minute, 5 minutes, 10 minutes, 12 minutes, 15 minutes, 30 minutes, 45 minutes, or 1 hour. In some embodiments, the step is achieved within 1 hour. In some embodiments, the step is achieved overnight. 
     In another aspect, a method for analyzing a bacterial sample can include:
         contacting at least one encoding probe with the sample to produce a first complex, wherein each encoding probe comprises a targeting sequence, a first landing pad sequence, and a second landing pad sequence;   adding at least one first emissive readout probe to the first complex, wherein the first emissive readout probe comprises a label and a sequence complementary to the first landing pad sequence;   detecting the first emissive readout probe with a confocal microscope;   adding an exchange probe to the sample, wherein the exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequence,   hybridizing the exchange probe to the first emissive readout probe to form a second complex;   removing the second complex from the sample,   adding at least one second emissive readout probe to the first complex, wherein the second emissive readout probe comprises a label and a sequence complementary to the second landing pad sequence;   detecting the second emissive readout probe with a confocal microscope;   repeating the aforementioned steps for at least one different encoding probe;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a bacterium; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     In another aspect, a method for analyzing a bacterial sample, comprising:
         generating a set of probes, wherein each probe comprises:   (i) a targeting sequence;   (ii) a first landing pad sequence; and   (iii) a second landing pad sequence;   contacting the set of probes with the sample to permit hybridization of the probes to nucleotides present in the sample to produce a complex;   adding a first set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   detecting the first set of emissive readout probes in the sample with a confocal microscope;   adding a set of exchange probes to the sample, wherein each exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequences,   hybridizing the exchange probes to the first emissive readout probes to form a second complex;   removing the second complex from the sample,   adding a second set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   detecting the second set of emissive readout probes in the sample with a confocal microscope;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a bacterium; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     Constructs and Libraries 
     In another aspect, a construct can include:
         a targeting sequence that is a region of interest on a nucleotide;   a first landing pad sequence;   a second landing pad sequence, wherein the second landing pad sequence is different from the first landing pad sequence;   a first emissive readout probe comprising a first label and a sequence complimentary to the first landing pad sequence;   an exchange probe comprising a 100% complementary sequence to the first emissive readout probe sequences; and   a second emissive readout probe comprising a second label and a sequence complimentary to the second landing pad sequence.       

     In another aspect, a library of constructs comprising a plurality of barcoded probes, wherein each barcoded probe can include:
         a targeting sequence that is a region of interest on a nucleotide;   a first landing pad sequence;   a second landing pad sequence, wherein the second landing pad sequence is different from the first landing pad sequence;   a first emissive readout probe comprising a first label and a sequence complimentary to the first landing pad sequence;   an exchange probe comprising a 100% complementary sequence to the first emissive readout probe sequences; and   a second emissive readout probe comprising a second label and a sequence complimentary to the second landing pad sequence.       

     In some embodiments, the region of interest on a nucleotide is at least one of messenger RNA (mRNA), microRNA (miRNA), long non coding RNA (lncRNA), ribosomal RNA (rRNA), small interfering RNA (siRNA), transfer RNA (tRNA), Crispr RNA (crRNA), trans-activating CRISPR RNA (tracrRNA), mitochondrial RNA, intronic RNA, viral mRNA, viral genomic RNA, environmental RNA, double-stranded RNA (dsRNA), small nuclear RNA (snRNA), small nucleolar (snoRNA), PIWI-interacting RNA (piRNA), genomic DNA, synthetic DNA, DNA, plasmid DNA, a plasmid, viral DNA, retroviral DNA, environmental DNA, extracellular DNA, a protein, a small molecule, or an antigen. 
     In some embodiments, the region of interest on a nucleotide is mRNA. 
     In some embodiments, the region of interest on a nucleotide is rRNA. 
     In some embodiments, the region of interest on a nucleotide is mRNA and rRNA. 
     In some embodiments, the first and second landing pad sequences have the same sequence. In some embodiments, the first and second landing pad sequences have different sequences. 
     In some embodiments, the first and second landing pad sequences each are about 10 to about 50, about 10 to about 40, about 10 to about 30, about 15 to about 25, about 18 to about 23, about 15, 16, 17, 18, 19, 20, 21, 22, or 23 nucleotides long. In some embodiments, the first and second landing pad sequences each are substantially complementary to the first and/or second emissive readout sequences. 
     In some embodiments, the first and second emissive readout probes are each about 10 to about 50, about 10 to about 40, about 10 to about 30, about 15 to about 25, about 18 to about 23, about 15, 16, 17, 18, 19, 20, 21, 22, or 23 nucleotides long bound with one of ten fluorescent dyes at the 5′- and/or 3′-end. In some embodiments, the first and second emissive readout probes each comprise a label and a sequence complementary to the first or second landing pad sequence. 
     In some embodiments, the first and second emissive readout probes are each of the same length as the corresponding landing pad sequence. In some embodiments, the first and second emissive readout probes are each 0 nucleotides longer than the corresponding landing pad sequence. In some embodiments, the first and second emissive readout probes are each at least 2 to 50 nucleotides longer than the corresponding landing pad sequence. In some embodiments, the first and second emissive readout probes are each at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, or 50 nucleotides longer than the corresponding landing pad sequence. In some embodiments, the first and second emissive readout probes are each at least 1, 2, 3, 4, or 5 nucleotides longer than the corresponding landing pad sequence. In some embodiments, the first and second emissive readout probes are each at least 5 nucleotides longer than the corresponding landing pad sequence. 
     In some embodiments, the readout sequence of the first and second emissive readout probes are each about 15 to about 50, about 15 to about 45, about 15 to about 35, about 15 to about 30, about 18 to about 24, about 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 nucleotides long. 
     In some embodiments, the emissive readout probe can include a label on the 5′ or 3′ end. In some embodiments, the emissive readout probe can include a label on the 5′ end and a label on the 3′ end. In some embodiments, the emissive readout probe can contain internal labels which may be the same or different. In some embodiments, the labels are the same. In some embodiments, the labels are different. 
     In some embodiments, the label is a fluorescent entity (fluorophore) or phosphorescent entity. In some embodiments, the label is a cyanine dye (e.g., Cy2, Cy3, Cy3B, Cy5, Cy5.5, Cy7, etc.), Alexa Fluor dye, Atto dye, photo switchable dye, photoactivatable dye, fluorescent dye, metal nanoparticle, semiconductor nanoparticle or “quantum dots”, fluorescent protein such as GFP (Green Fluorescent Protein), or photoactivatable fluorescent protein, such as PAGFP, PSCFP, PSCFP2, Dendra, Dendra2, EosFP, tdEos, mEos2, mEos3, PAmCherry, PAtagRFP, mMaple, mMaple2, and mMaple3. 
     In some embodiments, the label is Alexa Fluor 350, Alexa Fluor 405, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 514, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 561, Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 610, Alexa Fluor 633, Alexa Fluor 635, Alexa Fluor 647, Alexa Fluor 647-R-phycoerythrin, Alexa Fluor 660, Alexa Fluor 680, Alexa Fluor 680-allophycocyanin, Alexa Fluor 700, Alexa Fluor 750, Alexa Fluor 790, Alexa Fluor Plus 405, Alexa Fluor Plus 488, Alexa Fluor Plus 555, Alexa Fluor Plus 594, Alexa Fluor Plus 647, Alexa Fluor Plus 680, Alexa Fluor Plus 750, Alexa Fluor Plus 800, Pacific Blue, Pacific Green, Rhodamine Red X, DyLight 485-LS, DyLight-510-LS, DyLight 515-LS, DyLight 521-LS, Hydroxycoumarin, methoxycoumarin, Cy2, FAM, Fluorescein FITC, R-phycoerythrin (PE), Tamara, Cy3.5 581, Rox, Red 613, Texas Red, Cy5, Cy5.5, Cy7, Allophycocyanin, ATTO 430LS, ATTO 490LS, ATTO 390, ATTO 425, Cyan 500 NHS-Ester, ATTO 465, ATTO 488, ATTO 495, ATTO Rho110, ATTO 514, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 542, ATTO 550, ATTO 565, ATTO Rho3B, ATTO Rho11, ATTO Rho12, ATTO Thio12, ATTO Rho101, ATTO 590, ATTO 594, ATTO Rho13, ATTO 610, ATTO 620, ATTO Rho14, ATTO 633, ATTO 643, ATTO 647, ATTO 647N, ATTO 655, ATTO Oxa12, ATTO 665, ATTO 680, ATTO 700, ATTO 725, ATTO 740. 
     EMBODIMENTS 
     Embodiments of the present subject matter disclosed herein may be beneficial alone or in combination with one or more other embodiments. Without limiting the foregoing description, certain non-limiting embodiments of the disclosure, numbered I-1 to 11-37 are provided below. As will be apparent to those of skill in the art upon reading this disclosure, each of the individually numbered embodiments may be used or combined with any of the preceding or following individually numbered embodiments. This is intended to provide support for all such combinations of embodiments and is not limited to combinations of embodiments explicitly provided below. 
     Embodiments of the Disclosure 
     Embodiment I-1: A method of characterizing a microbial cell from a biological sample, the method comprising a) directly inoculating the microbe onto a device; b) identifying the microbe; and c) detecting susceptibility to one or more antimicrobial agents. 
     Embodiment II-1: A method of characterizing a microbial cell from a biological sample, the method comprising a) directly inoculating the microbe onto a device; b) identifying the microbe; and c) detecting future susceptibility to one or more antimicrobial agents. 
     Embodiment II-2: The method of embodiments I-1 or II-1, wherein the sample is not subjected to culturing before the microbe is inoculated onto the device. 
     Embodiment II-3: The method of embodiments I-1 or II-1 to II-2, wherein the microbe in the sample is cultured for one to 12 cell divisions before it is inoculated onto the device. 
     Embodiment II-4: The method of embodiments I-1 or II-1 to II-3, wherein the microbe is identified by in situ hybridization. 
     Embodiment II-5: The method of embodiments I-1 or II-1 to II-4, wherein the microbe is identified by fluorescence in situ hybridization (FISH). 
     Embodiment II-6: The method of embodiments I-1 or II-1 to II-5, wherein the fluorescence in situ hybridization is high-phylogenetic-resolution fluorescence in situ hybridization (HiPR-FISH). 
     Embodiment II-7: The method of embodiments I-1 or II-1 to II-6, wherein the microbe is further characterized via live-cell imaging or dynamic calculation while in situ hybridization is performed. 
     Embodiment II-8: The method of embodiments I-1 or II-1 to II-7, wherein the microbe is identified by hybridization of a bar-coded probe a 16S ribosomal RNA sequence in the microbe, 5S ribosomal RNA sequence in the microbe, and/or 23S ribosomal RNA sequence in the microbe. 
     Embodiment II-9: The method of embodiments I-1 or II-1 to II-8, wherein the in situ hybridization is multiplexed. 
     Embodiment II-10: The method of embodiments I-1 or II-1 to II-9, wherein the susceptibility to one or more microbial agents is determined by measuring the minimum inhibitory concentration of the microbe when exposed to an antimicrobial agent. 
     Embodiment II-11: The method of embodiments I-1 or II-1 to II-10, wherein the susceptibility to one or more microbial agents is determined by measuring microbial cell metabolism when the microbe is exposed to an antimicrobial agent. 
     Embodiment II-12: The method of embodiments I-1 or II-1 to II-11, wherein microbial cell metabolism is measured by determining the concentration of dissolved carbon dioxide, oxygen consumption of microbes in the sample, expression of genes involved in cell division and/or growth, or expression of stress response genes. 
     Embodiment II-13: The method of embodiments I-1 or II-1 to II-12, wherein microbial cell susceptibility is determined by a live/dead stain. 
     Embodiment II-14: The method of embodiments I-1 or II-1 to II-13, wherein microbial cell susceptibility is determined by cell number. 
     Embodiment II-15: The method of embodiments I-1 or II-1 to II-14, wherein microbial cell susceptibility is determined by detecting the presence or absence of one or more antimicrobial genes in the microbial cell. 
     Embodiment II-16: The method of embodiments I-1 or II-1 to II-15, wherein microbial cell susceptibility is determined by detecting the presence or absence of one or more gene mutations associated with the development of antimicrobial resistance or susceptibility in the microbial cell. 
     Embodiment II-17: The method of embodiments I-1 or II-1 to II-16, wherein future microbial cell susceptibility is determined by detecting the presence or absence of one or more antimicrobial genes in the microbial cell. 
     Embodiment II-18: The method of embodiments I-1 or II-1 to II-17, wherein future microbial cell susceptibility is determined by detecting the presence or absence of one or more gene mutations associated with the development of antimicrobial resistance or susceptibility in the microbial cell. 
     Embodiment II-19: The method of embodiments I-1 or II-1 to II-18, wherein the one or more gene mutations associated with the development of antimicrobial resistance or susceptibility is selected from deletions, duplications, single nucleotide polymorphisms (SNPs), frame-shift mutations, inversions, insertions, and/or nucleotide substitutions. 
     Embodiment II-20: The method of embodiments I-1 or II-1 to II-19, wherein the one or more antimicrobial genes is selected from: genes encoding multidrug resistance proteins (e.g. PDR1, PDR3, PDR7, PDR9), ABC transporters (e.g. SNQ2, STE6, PDR5, PDR10, PDR11, YOR1), membrane associated transporters (GAS1, D4405), soluble proteins (e.g. G3PD), RNA polymerase, rpoB, gyrA, gyrB, 16S RNA, 23S rRNA, NADPH nitroreductase, sul2, strAB, tetAR, aac3-iid, aph, sph, cmy-2, floR, tetB; aadA, aac3-VIa, and sul1. 
     Embodiment II-21: The method of embodiments I-1 or II-1 to II-20, wherein the presence or absence of one or more antimicrobial genes, or the gene mutation associated with the development of antimicrobial resistance or susceptibility in the microbial cell is detected using in situ hybridization. 
     Embodiment II-22: The method of embodiments I-1 or II-1 to II-21, wherein the presence or absence of one or more antimicrobial genes, or the gene mutation associated with the development of antimicrobial resistance or susceptibility in the microbial cell is detected using fluorescence in situ hybridization (FISH). 
     Embodiment II-23: The method of embodiments I-1 or II-1 to II-22, wherein the fluorescence in situ hybridization is high-phylogenetic-resolution fluorescence in situ hybridization (HiPR-FISH). 
     Embodiment II-24: The method of embodiments I-1 or II-1 to II-23, wherein the identification of the microbial cell and the detection of susceptibility or future susceptibility to one or more antimicrobial agents occurs sequentially. 
     Embodiment II-25: The method of embodiments I-1 or II-1 to II-24, wherein the identification of the microbial cell and the detection of susceptibility or future susceptibility to one or more antimicrobial agents occurs simultaneously. 
     Embodiment II-26: The method of embodiments I-1 or II-1 to II-25, wherein the identification of the microbial cell and the detection of susceptibility or future susceptibility to one or more antimicrobial agents occurs in parallel. 
     Embodiment II-27: The method of embodiments I-1 or II-1 to II-26, wherein the biological sample is obtained from a patient. 
     Embodiment II-28: The method of embodiments I-1 or II-1 to II-27, wherein the biological sample is obtained from a patient diagnosed with or believed to be suffering from an infection or disorder. 
     Embodiment II-29: The method of embodiments I-1 or II-1 to II-28, wherein the disease or disorder is an infection. 
     Embodiment II-30: The method of embodiments I-1 or II-1 to II-29, wherein the infection is a bacterial, viral, fungal, or parasitic infections. 
     Embodiment II-31: The method of embodiments I-1 or II-1 to II-30, wherein the bacterial infection is selected from  Mycobacterium, Streptococcus, Staphylococcus, Shigella, Campylobacter, Salmonella, Clostridium, Corynebacterium, Pseudomonas, Neisseria, Listeria, Vibrio, Bordetella, E. coli  (including pathogenic  E. coli ),  Pseudomonas aeruginosa, Enterobacter cloacae, Mycobacterium tuberculosis, Staphylococcus aureus, Helicobacter pylori, Legionella, Acinetobacter baumannii, Citrobacter freundii, Citrobacter koseri, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus saprophyticus , and  Streptococcus agalactiae , or a combination thereof. 
     Embodiment II-32: The method of embodiments I-1 or II-1 to II-30, wherein the viral infection is selected from  Helicobacter pylori , infectious haematopoietic necrosis virus (IHNV), Parvovirus B19, Herpes Simplex Virus, Varicella-zoster virus, Cytomegalovirus, Epstein-Barr virus, Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Hepatitis D virus, Hepatitis E virus, Measles virus, Mumps virus, Rubella virus, Human Immunodeficiency Virus (HIV), Influenza virus, Rhinovirus, Rotavirus A, Rotavirus B, Rotavirus C, Respiratory Syncytial Virus (RSV), Varicella zoster, Poliovirus, Norovirus, Zika Virus, Dengue Virus, Rabies Virus, Newcastle Disease Virus, and White Spot Syndrome Virus, or a combination thereof. 
     Embodiment II-33: The method of embodiments I-1 or II-1 to II-30, wherein the fungal infection is selected from  Aspergillus, Candida, Pneumocystis, Blastomyces, Coccidioides, Cryptococcus , and  Histoplasma , or a combination thereof. 
     Embodiment II-34: The method of embodiments I-1 or II-1 to II-30, wherein the parasitic infection is selected from  Plasmodium  (i.e.  P. falciparum, P. malariae, P. ovale, P. knowlesi , and  P. vivax ),  Trypanosoma, Toxoplasma, Giardia , and  Leishmania, Cryptosporidium , helminthic parasites:  Trichuris  spp. (whipworms),  Enterobius  spp. (pinworms),  Ascaris  spp. (roundworms),  Ancylostoma  spp. and  Necator  spp. (hookworms),  Strongyloides  spp. (threadworms),  Dracunculus  spp. (Guinea worms),  Onchocerca  spp. and  Wuchereria  spp. (filarial worms),  Taenia  spp.,  Echinococcus  spp., and  Diphyllobothrium  spp. (human and animal cestodes),  Fasciola  spp. (liver flukes) and  Schistosoma  spp. (blood flukes), or a combination thereof. 
     Embodiment II-35: The method of embodiments I-1 or II-1 to II-34, wherein the biological sample is selected from bronchoalveolar lavage fluid (BAL), blood, serum, plasma, urine, cerebrospinal fluid, pleural fluid, synovial fluid, ocular fluid, peritoneal fluid, amniotic fluid, gastric fluid, lymph fluid, interstitial fluid, tissue homogenate, cell extracts, saliva, sputum, stool, physiological secretions, tears, mucus, sweat, milk, semen, seminal fluid, vaginal secretions, fluid from ulcers and other surface eruptions, blisters, and abscesses, and extracts of tissues including biopsies of normal, malignant, and suspect tissues or any other constituents of the body which may contain the microorganism of interest. 
     Embodiment II-36: The method of embodiments I-1 or II-1 to II-34, wherein the biological sample is a human oral microbiome sample. 
     Embodiment II-37: The method of embodiments I-1 or II-1 to II-34, wherein the biological sample is a whole organism. 
     Embodiment III-1: A method for analyzing a sample, comprising:
         contacting at least one encoding probe with the sample to produce a first complex, wherein each encoding probe comprises a targeting sequence, a first landing pad sequence, and a second landing pad sequence;   adding at least one first emissive readout probe to the first complex, wherein the first emissive readout probe comprises a label and a sequence complementary to the first landing pad sequence;   acquiring one or more emission spectra from the first emissive readout probe;   adding an exchange probe to the sample, wherein the exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequence,   hybridizing the exchange probe to the first emissive readout probe to form a second complex;   removing the second complex from the sample,   adding at least one second emissive readout probe to the first complex, wherein the second emissive readout probe comprises a label and a sequence complementary to the second landing pad sequence;   acquiring one or more emission spectra from the second emissive readout probe;   repeating the aforementioned steps for at least one different encoding probe;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a species of interest; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     Embodiment IV-1: A method for analyzing a sample, comprising:
         generating a set of probes, wherein each probe comprises:   (i) a targeting sequence;   (ii) a first landing pad sequence; and   (iii) a second landing pad sequence;   contacting the set of probes with the sample to permit hybridization of the probes to nucleotides present in the sample to produce a complex;   adding a first set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   acquiring one or more emission spectra from the first emissive readout probe;   adding a set of exchange probes to the sample, wherein each exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequences,   hybridizing the exchange probes to the first emissive readout probes to form a second complex;   removing the second complex from the sample,   adding a second set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   acquiring one or more emission spectra from the second emissive readout probe;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a species of interest; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     Embodiment IV-2: The method of embodiments III-1 or IV-1, wherein the sample is at least one of a cell, a cell suspension, a tissue biopsy, a tissue specimen, urine, stool, blood, serum, plasma, bone biopsies, bone marrow, respiratory specimens, sputum, induced sputum, tracheal aspirates, bronchoalveolar lavage fluid, sweat, saliva, tears, ocular fluid, cerebral spinal fluid, pericardial fluid, pleural fluid, peritoneal fluid, placenta, amnion, pus, nasal swabs, nasopharyngeal swabs, oropharyngeal swabs, ocular swabs, skin swabs, wound swabs, mucosal swabs, buccal swabs, vaginal swabs, vulvar swabs, nails, nail scrapings, hair follicles, corneal scrapings, gavage fluids, gargle fluids, abscess fluids, wastewater, or plant biopsies. 
     Embodiment IV-3: The method of embodiment IV-2, wherein the sample is a cell. 
     Embodiment IV-4: The method of embodiment IV-3, wherein the cell is a bacterial or eukaryotic cell. 
     Embodiment IV-5: The method of embodiment IV-2, wherein the sample comprises a plurality of cells. 
     Embodiment IV-6: The method of embodiment IV-5, wherein each cell comprises a specific targeting sequence. 
     Embodiment IV-7: The method of Embodiments III-1 or IV-1, wherein the targeting sequence targets at least one of messenger RNA (mRNA), microRNA (miRNA), long non-coding RNA (lncRNA), ribosomal RNA (rRNA), small interfering RNA (siRNA), transfer RNA (tRNA), Crispr RNA (crRNA), trans-activating CRISPR RNA (tracrRNA), mitochondrial RNA, intronic RNA, viral mRNA, viral genomic RNA, environmental RNA, double-stranded RNA (dsRNA), small nuclear RNA (snRNA), small nucleolar (snoRNA), PIWI-interacting RNA (piRNA), genomic DNA, synthetic DNA, DNA, plasmid DNA, a plasmid, viral DNA, retroviral DNA, environmental DNA, extracellular DNA, a protein, a small molecule, or an antigenic target. 
     Embodiment IV-8: The method of embodiment IV-7, wherein the target is mRNA. 
     Embodiment IV-9: The method of embodiment IV-7, wherein the target is rRNA. 
     Embodiment IV-10: The method of embodiment IV-7, wherein the target is mRNA and rRNA. 
     Embodiment IV-11: The method of Embodiments III-1 or IV-1, wherein the at least one encoding probe comprises the first landing pad sequence on the 5′ end, and the second landing pad sequence on the 3′ end. 
     Embodiment IV-12: The method of Embodiments III-1 or IV-1, wherein the at least one encoding probe comprises the first landing pad sequence on the 3′ end, and the second landing pad sequence on the 5′ end. 
     Embodiment IV-13: The method of embodiment IV-12, wherein the first landing pad sequence and the second landing pad sequences have different sequences. 
     Embodiment IV-14: The method of Embodiments III-1 or IV-1, wherein the at least one first or second emissive readout probe comprises a label on the 5′ or 3′ end. 
     Embodiment IV-15: The method of Embodiments III-1 or IV-1, wherein the label is Alexa Fluor 350, Alexa Fluor 405, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 514, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 561, Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 610, Alexa Fluor 633, Alexa Fluor 635, Alexa Fluor 647, Alexa Fluor 647-R-phycoerythrin, Alexa Fluor 660, Alexa Fluor 680, Alexa Fluor 680-allophycocyanin, Alexa Fluor 700, Alexa Fluor 750, Alexa Fluor 790, Alexa Fluor Plus 405, Alexa Fluor Plus 488, Alexa Fluor Plus 555, Alexa Fluor Plus 594, Alexa Fluor Plus 647, Alexa Fluor Plus 680, Alexa Fluor Plus 750, Alexa Fluor Plus 800, Pacific Blue, Pacific Green, Rhodamine Red X, DyLight 485-LS, DyLight-510-LS, DyLight 515-LS, DyLight 521-LS, Hydroxycoumarin, methoxycoumarin, Cy2, FAM, Fluorescein FITC, R-phycoerythrin (PE), Tamara, Cy3.5 581, Rox, Red 613, Texas Red, Cy5, Cy5.5, Cy7, Allophycocyanin, ATTO 430LS, ATTO 490LS, ATTO 390, ATTO 425, Cyan 500 NHS-Ester, ATTO 465, ATTO 488, ATTO 495, ATTO Rho110, ATTO 514, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 542, ATTO 550, ATTO 565, ATTO Rho3B, ATTO Rho11, ATTO Rho12, ATTO Thio12, ATTO Rho101, ATTO 590, ATTO 594, ATTO Rho13, ATTO 610, ATTO 620, ATTO Rho14, ATTO 633, ATTO 643, ATTO 647, ATTO 647N, ATTO 655, ATTO Oxa12, ATTO 665, ATTO 680, ATTO 700, ATTO 725, ATTO 740. 
     Embodiment IV-16: The method of Embodiments III-1 or IV-1, wherein the one or more emission spectra of the first and/or second emissive readout probe is acquired via widefield microscopy, point scanning confocal microscopy, spinning disk confocal microscopy, lattice lightsheet microscopy, or light field microscopy. 
     Embodiment IV-17: The method of embodiment IV-17, wherein the detection strategy used is channel, spectral, channel and fluorescence lifetime, or spectral and fluorescence lifetime. 
     Embodiment IV-18: The method of Embodiments III-1 or IV-1, wherein the sample is on an analyzing platform, wherein the analyzing platform is a microscope slide, at least one chamber, at least one microfluidic device, at least one well, at least one plate, or at least one filter membrane. 
     Embodiment IV-19: The method of Embodiments III-1 or IV-1, wherein adding an exchange probe to the sample, hybridizing the exchange probe to the first emissive readout probe, and removing the second complex from the sample are performed in the same step. 
     Embodiment IV-20: The method of Embodiments III-1 or IV-1, wherein hybridizing the exchange probe to the first or second emissive readout probe results in de-hybridization of the first or second emissive readout probe from the first or second landing pad sequence. 
     Embodiment IV-21: The method of embodiments IV-19 or IV-20, wherein the step is achieved within 1 hour. 
     Embodiment IV-22: The method of embodiments IV-19 or IV-20, wherein the step is achieved overnight. 
     Embodiment IV-23: The method of any one of embodiments III-1, or IV-1 to IV-22, wherein the emissive readout probe sequence is at least 5 nucleotides longer than the first or second landing pad sequences. 
     Embodiment V-1: A construct comprising:
         a targeting sequence that is a region of interest on a nucleotide;   a first landing pad sequence;   a second landing pad sequence, wherein the second landing pad sequence is different from the first landing pad sequence;   a first emissive readout probe comprising a first label and a sequence complimentary to the first landing pad sequence;   an exchange probe comprising a 100% complementary sequence to the first emissive readout probe sequences; and   a second emissive readout probe comprising a second label and a sequence complimentary to the second landing pad sequence.       

     Embodiment VI-1: A library of constructs comprising a plurality of barcoded probes, wherein each barcoded probe comprises:
         a targeting sequence that is a region of interest on a nucleotide;   a first landing pad sequence;   a second landing pad sequence, wherein the second landing pad sequence is different from the first landing pad sequence;   a first emissive readout probe comprising a first label and a sequence complimentary to the first landing pad sequence;   an exchange probe comprising a 100% complementary sequence to the first emissive readout probe sequences; and   a second emissive readout probe comprising a second label and a sequence complimentary to the second landing pad sequence.       

     Embodiment VI-2: The construct of embodiments V-1 or VI-2, wherein the first emissive readout probe sequence is at least 5 nucleotides longer than the first landing pad sequence. 
     Embodiment VI-3: The construct of embodiments V-1 or VI-2, wherein the second emissive readout probe sequence is at least 5 nucleotides longer than the second landing pad sequence. 
     Embodiment VI-4: The construct of embodiments V-1 or VI-2, wherein the first landing pad sequence and the second landing pad sequences have different sequences. 
     Embodiment VI-5: The construct of embodiments V-1 or VI-2, wherein the first emissive readout probe comprises the first label on the 5′ or 3′ end. 
     Embodiment VI-6: The construct of embodiments V-1 or VI-2, wherein the second emissive readout probe comprises the second label on the 5′ or 3′ end. 
     Embodiment VI-7: The construct of embodiments V-1 or VI-2, wherein the first or second label is each Alexa Fluor 350, Alexa Fluor 405, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 514, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 561, Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 610, Alexa Fluor 633, Alexa Fluor 635, Alexa Fluor 647, Alexa Fluor 647-R-phycoerythrin, Alexa Fluor 660, Alexa Fluor 680, Alexa Fluor 680-allophycocyanin, Alexa Fluor 700, Alexa Fluor 750, Alexa Fluor 790, Alexa Fluor Plus 405, Alexa Fluor Plus 488, Alexa Fluor Plus 555, Alexa Fluor Plus 594, Alexa Fluor Plus 647, Alexa Fluor Plus 680, Alexa Fluor Plus 750, Alexa Fluor Plus 800, Pacific Blue, Pacific Green, Rhodamine Red X, DyLight 485-LS, DyLight-510-LS, DyLight 515-LS, DyLight 521-LS, Hydroxycoumarin, methoxycoumarin, Cy2, FAM, Fluorescein FITC, R-phycoerythrin (PE), Tamara, Cy3.5 581, Rox, Red 613, Texas Red, Cy5, Cy5.5, Cy7, Allophycocyanin, ATTO 430LS, ATTO 490LS, ATTO 390, ATTO 425, Cyan 500 NHS-Ester, ATTO 465, ATTO 488, ATTO 495, ATTO Rho110, ATTO 514, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 542, ATTO 550, ATTO 565, ATTO Rho3B, ATTO Rho11, ATTO Rho12, ATTO Thio12, ATTO Rho101, ATTO 590, ATTO 594, ATTO Rho13, ATTO 610, ATTO 620, ATTO Rho14, ATTO 633, ATTO 643, ATTO 647, ATTO 647N, ATTO 655, ATTO Oxa12, ATTO 665, ATTO 680, ATTO 700, ATTO 725, or ATTO 740. 
     Embodiment VII-1: A method for analyzing a bacterial sample, comprising:
         contacting at least one encoding probe with the sample to produce a first complex, wherein each encoding probe comprises a targeting sequence, a first landing pad sequence, and a second landing pad sequence;   adding at least one first emissive readout probe to the first complex, wherein the first emissive readout probe comprises a label and a sequence complementary to the first landing pad sequence;   detecting the first emissive readout probe with a confocal microscope;   adding an exchange probe to the sample, wherein the exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequence,   hybridizing the exchange probe to the first emissive readout probe to form a second complex;   removing the second complex from the sample,   adding at least one second emissive readout probe to the first complex, wherein the second emissive readout probe comprises a label and a sequence complementary to the second landing pad sequence;   detecting the second emissive readout probe with a confocal microscope;   repeating the aforementioned steps for at least one different encoding probe;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a bacterium; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     Embodiment VIII-1: A method for analyzing a bacterial sample, comprising:
         generating a set of probes, wherein each probe comprises:   (i) a targeting sequence;   (ii) a first landing pad sequence; and   (iii) a second landing pad sequence;   contacting the set of probes with the sample to permit hybridization of the probes to nucleotides present in the sample to produce a complex;   adding a first set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   detecting the first set of emissive readout probes in the sample with a confocal microscope;   adding a set of exchange probes to the sample, wherein each exchange probe comprises a 100% complementary sequence to the first emissive readout probe sequences,   hybridizing the exchange probes to the first emissive readout probes to form a second complex;   removing the second complex from the sample,   adding a second set of emissive readout probes to the complex, wherein each emissive readout probe comprises:   (i) a label, and   (ii) a sequence complementary to the first or second landing pad sequence;   detecting the second set of emissive readout probes in the sample with a confocal microscope;   determining the spectra of “signal” (e.g., puncta, blobs) and assigning them to a bacterium; and   decoding the spectra into a single, targeted transcript through means of signal deconvolution, error correction, comparison to reference standards.       

     EXAMPLES 
     Abbreviations and Definitions 
       
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 Abbreviation 
                 Definition 
               
               
                   
                   
               
             
            
               
                   
                 SSC 
                 sodium chloride sodium citrate 
               
               
                   
                 SSCT 
                 2x SSC + 0.1% Tween 20 
               
               
                   
                 SDS 
                 Sodium dodecyl sulfate 
               
               
                   
                 EDTA 
                 Ethylenediaminetetraacetic acid 
               
               
                   
                 Tris HCl 
                 Tris Hydrochloride 
               
               
                   
                   
                 (tris(hydroxymethyl)aminomethane 
               
               
                   
                   
                 hydrochloride) 
               
               
                   
                 NaCl 
                 Sodium chloride 
               
               
                   
                 PBS 
                 Phosphate-buffered saline 
               
               
                   
                 RT 
                 Room temperature 
               
               
                   
                   
               
            
           
         
       
     
     Example 1. Identification and Antimicrobial Susceptibility Characterization of Microbes 
     To enable parallel measurement of cellular state at different antibiotic concentrations, microbial cells are colocalized with a volume of antibiotic solution with a known concentration. This objective can potentially be achieved in several ways. 
     Solution 1: a buffer containing cells are applied to a plate with microfabricated wells (well size can be hundreds of microns to millimeters). Cells may be allowed to settle into individual wells by gravity or by centrifugation. After cell settlement, excess solutions are removed. Subsequently, a hydrated gel (agar, agarose, polyethylene glycol, or polyacrylamide, for example) loaded with an antimicrobial gradient can be applied over the top of the plate, allowing different wells to be exposed to different concentrations of antimicrobial compounds. Solution 2: a buffer containing cells are passed through a microfluidic device to convert the bulk solution into a solution of droplets, where each droplet may contain zero or more cells. The cell droplets are then merged with droplets of antimicrobial solutions using a second microfluidic device, allowing different cells to be exposed to antimicrobial solutions at different concentrations. The antimicrobial solution can be colored with food coloring, or other bacteria-compatible dyes, to allow them to be distinguished on an imaging device. Solution 3: a buffer containing cells are microencapsulated into semipermeable polymeric beads. The polymer beads containing microbial cells are then distributed into wells on a plate, where each well contains a known concentration of antimicrobial compounds. 
     Example 2. Identification of Microbes in Patient Sample 
     The methods of the disclosure were used to identify microbes and drug-resistance phenotype in patient urine samples. The experimental set up is shown in  FIG.  2   . A 75-well plate is created with 2-fold dilution series of ten different antibiotics, and the urine samples collected from the patients were deposited over each well. The plate was incubated for 2 hours, and fixed, and HiPR-FISH was performed. Samples were tested at time 0 and 2 hours of the incubation as shown in  FIG.  5   . After this process (about 4 hours total), spectral imaging was used to identify the microbial species in the patient sample. The detection panel used here detects and differentiates between the following bacteria:
           Acinetobacter baumannii        Citrobacter freundii        Citrobacter koseri        Enterobacter cloacae        Enterococcus faecalis        Enterococcus faecium        Escherichia coli        Klebsiella oxytoca        Klebsiella pneumoniae        Proteus mirabilis        Proteus vulgaris        Pseudomonas aeruginosa        Serratia marcescens        Staphylococcus aureus        Staphylococcus saprophyticus        Streptococcus agalactiae          

     Example 2.1 
       FIG.  3    shows the identification of  E. coli  in three different patient samples using the following methodology. 
     Specimens were stored in a mixture of urine supernatant and glycerol and frozen at −80° C. until time of processing. Specimens were thawed and deposited onto the device and incubated at 37° C. for one hour. The specimen was biologically fixed by depositing 2% formaldehyde onto the specimen and incubated for thirty minutes at room temperature. The specimens were washed using 1×PBS multiple times at room temperature. An encoding buffer (2×SSC, 10% dextran sulfate, 10% ethylene carbonate, 5×Denhardt&#39;s solution, 0.01% SDS) with probes designed for a panel of uropathogens (at roughly 200 nM per taxon) was deposited on cells and incubated for two hours at 37° C. A wash buffer (5 mM EDTA, 20 mM Tris HCl, 215 mM NaCl) was then deposited on specimens for fifteen minutes at 37° C. to remove unbound probes. A buffer containing readout probes (10 readout probes, each at 400 nM; buffer made up of 2×SSC, 10% dextran sulfate, 10% ethylene carbonate, 5×Denhardt&#39;s solution, 0.01% SDS) was incubated for 30 minutes at room temperature. A second round of wash buffer was deposited on specimens for fifteen minutes at 37° C. to remove unbound probe. The specimens were then suspended in 2×SSC and a coverslip was placed directly over the specimens for imaging on a confocal microscope. 
     Example 2.2 
       FIG.  4    shows the identification of different species including  A. baumannii, C. freundii, S. saprophyticus , and a mixture of  A. baumannii  and  C. freundii  using different excitation wavelengths, using the following methodology. 
     Suspensions of individual monocultures were fixed by adding an equal volume of 2% formaldehyde, mixing, and incubating for 90 minutes at room temperature. Fixed cultures were then washed with 1×PBS and resuspended in 50% ethanol. Single taxa suspensions or mixed suspensions containing multiple taxa, were deposited onto glass microscope slides until 50% ethanol had evaporated. Lysosyme (10 mg/mL) was deposited onto each dry specimen to permeabilize the outer membrane and incubated for 30 minutes at 37° C., the slides were then washed with 1×PBS. An encoding probe hybridization buffer (2×SSC, 10% dextran sulfate, 10% ethylene carbonate, 5×Denhardt&#39;s solution, 0.01% SDS) with probes designed for a panel of uropathogens (at roughly 200 nM per taxon) was deposited on cells and incubated for two hours at 37° C. A wash buffer (5 mM EDTA, 20 mM Tris HCl, 215 mM NaCl) was then deposited on specimens for fifteen minutes at 37° C. to remove unbound probes. A buffer containing readout probes (10 readout probes, each at 400 nM; buffer made up of 2×SSC, 10% dextran sulfate, 10% ethylene carbonate, 5×Denhardt&#39;s solution, 0.01% SDS) was incubated for one hour at room temperature. A second round of wash buffer was deposited on specimens for fifteen minutes at 37° C. to remove unbound probe. The specimens were mounted with Prolong Glass and a coverslip was placed directly over the specimens for imaging on confocal microscope. 
     Table 1 shows the sequences of the readout probes used various Examples disclosed herein. Table 2 shows the sequences of the encoding probes used in Examples 2.1 and 2.2. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Readout Probes 
               
            
           
           
               
               
               
            
               
                 SEQ 
                   
                   
               
               
                 ID 
                   
                 Sequence 
               
               
                 NO: 
                 Probe Name 
                 (in 5′ to 3′ order) 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 Readout Probe 1 
                 /5Alex488N/TATCCTTCAATCCC 
               
               
                   
                   
                 TCCACA 
               
               
                   
               
               
                 2 
                 Readout Probe 2 
                 /5Alex546N/ACACTACCACCATT 
               
               
                   
                   
                 TCCTAT 
               
               
                   
               
               
                 3 
                 Readout Probe 3 
                 /56-ROXN/ACTCCACTACTACTCA 
               
               
                   
                   
                 CTCT/3Rox_N/ 
               
               
                   
               
               
                 4 
                 Readout Probe 4 
                 /5PacificGreenN/ACCCTCTA 
               
               
                   
                   
                 ACTTCCATCACA 
               
               
                   
               
               
                 5 
                 Readout Probe 5 
                 /5PacificBlueN/ACCACAACCC 
               
               
                   
                   
                 ATTCCTTTCA 
               
               
                   
               
               
                 6 
                 Readout Probe 6 
                 /5Atto610N/TTTACTCCCTACAC 
               
               
                   
                   
                 CTCAA 
               
               
                   
               
               
                 7 
                 Readout Probe 7 
                 /5Alex647N/ACCCTTTACAAACA 
               
               
                   
                   
                 CACCCT 
               
               
                   
               
               
                 8 
                 Readout Probe 8 
                 /5DyLight-510-LS/TCCTATTC 
               
               
                   
                   
                 TCAACCTAACCT/3DyLight-510- 
               
               
                   
                   
                 LS/ 
               
               
                   
               
               
                 9 
                 Readout Probe 9 
                 /5Alex405N/TTCTCCCTCTATCA 
               
               
                   
                   
                 ACTCTA 
               
               
                   
               
               
                 10 
                 Readout Probe 10 
                 /5Alex532N/ACCCTTACTACTAC 
               
               
                   
                   
                 ATCATC/3Alexa532N/ 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Encoding Probes used in Examples 2.1 and 2.2 
               
            
           
           
               
               
               
            
               
                 SEQ ID 
                   
                   
               
               
                 NO: 
                 Probe Name 
                 Sequence (in 5′ to 3′ order) 
               
               
                   
               
            
           
           
               
               
               
            
               
                 11 
                 Encoding Probe 1 
                 TGTGGAGGGATTGAAGGATACACCTCCTTGCTAT 
               
               
                   
                   
                 AGCCACCTTATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 12 
                 Encoding Probe 2 
                 TGTGGAGGGATTGAAGGATAGGCAACATCAGAG 
               
               
                   
                   
                 AAGCAAGCAAGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 13 
                 Encoding Probe 3 
                 TGTGGAGGGATTGAAGGATAAGCGACACAATGT 
               
               
                   
                   
                 CTTCTCCCGTATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 14 
                 Encoding Probe 4 
                 TGTGGAGGGATTGAAGGATATCTCAATGTCTTCT 
               
               
                   
                   
                 CCCCATCAGTCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 15 
                 Encoding Probe 5 
                 TGTGGAGGGATTGAAGGATACATGGCACCTATTT 
               
               
                   
                   
                 TCTATCTAGAGCGATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 16 
                 Encoding Probe 6 
                 TGTGGAGGGATTGAAGGATACTGGAAGACACAA 
               
               
                   
                   
                 TGTCTTCTCAGGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 17 
                 Encoding Probe 7 
                 TGTGGAGGGATTGAAGGATAGTCCAGCCTTAATG 
               
               
                   
                   
                 AGTACCGCTATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 18 
                 Encoding Probe 8 
                 TGTGGAGGGATTGAAGGATAGGATCGATTAAAA 
               
               
                   
                   
                 CGATTATAGGTGGATGTGTGGAGGGATTGAAGG 
               
               
                   
                   
                 ATA 
               
               
                   
               
               
                 19 
                 Encoding Probe 9 
                 TGTGGAGGGATTGAAGGATAGGACGATTAAAAC 
               
               
                   
                   
                 GATTATAGGTGGTTGTTGTGGAGGGATTGAAGGA 
               
               
                   
                   
                 TA 
               
               
                   
               
               
                 20 
                 Encoding Probe 10 
                 TGTGGAGGGATTGAAGGATAATTGACAGCAAGA 
               
               
                   
                   
                 CCGTCTTTGTGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 21 
                 Encoding Probe 11 
                 TGTGGAGGGATTGAAGGATAGATATTGTCCAAAG 
               
               
                   
                   
                 GACAATCCTGTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 22 
                 Encoding Probe 12 
                 TGTGGAGGGATTGAAGGATATTCACAATGTCTTC 
               
               
                   
                   
                 TCCCCATGTGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 23 
                 Encoding Probe 13 
                 TGTGGAGGGATTGAAGGATAGGATCACCCATGTT 
               
               
                   
                   
                 CTGACTCGGTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 24 
                 Encoding Probe 14 
                 TGTGGAGGGATTGAAGGATAATCCTCACGTTTCA 
               
               
                   
                   
                 AAGGCTCGATTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 25 
                 Encoding Probe 15 
                 TGTGGAGGGATTGAAGGATAAAGCGCTACCCTCA 
               
               
                   
                   
                 GTTCATCCCGATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 26 
                 Encoding Probe 16 
                 TGTGGAGGGATTGAAGGATAAAGCCTGACCAAG 
               
               
                   
                   
                 GGTAGATCTGGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 27 
                 Encoding Probe 17 
                 TGTGGAGGGATTGAAGGATATTGCAACCTGACCA 
               
               
                   
                   
                 AGGGTAGTAGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 28 
                 Encoding Probe 18 
                 TGTGGAGGGATTGAAGGATAGATATCAGAGAAG 
               
               
                   
                   
                 CAAGCTTCAGCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 29 
                 Encoding Probe 19 
                 TGTGGAGGGATTGAAGGATAAGGTCAAGAGAGA 
               
               
                   
                   
                 CAACATTTTCCTGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 30 
                 Encoding Probe 20 
                 TGTGGAGGGATTGAAGGATACTATTCGTCTAATG 
               
               
                   
                   
                 TCGTCCTTTCATTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 31 
                 Encoding Probe 21 
                 TGTGGAGGGATTGAAGGATATGACTAATGCAGC 
               
               
                   
                   
                 GCGGATCCTAGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 32 
                 Encoding Probe 22 
                 TGTGGAGGGATTGAAGGATATATTGACAGCAAG 
               
               
                   
                   
                 ACCGTCTTAGTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 33 
                 Encoding Probe 23 
                 TGTGGAGGGATTGAAGGATACAGCCGCTAACATC 
               
               
                   
                   
                 AGAGAAGCTTCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 34 
                 Encoding Probe 24 
                 TGTGGAGGGATTGAAGGATACAGCTCCACATGTC 
               
               
                   
                   
                 ACCATGCAAGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 35 
                 Encoding Probe 25 
                 TGTGGAGGGATTGAAGGATACAAAAAGCCAACA 
               
               
                   
                   
                 CAGCTAGGCATTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 36 
                 Encoding Probe 26 
                 ATAGGAAATGGTGGTAGTGTGATCAACAACGCAT 
               
               
                   
                   
                 AAGCGTCGCACGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 37 
                 Encoding Probe 27 
                 ATAGGAAATGGTGGTAGTGTGGACCAACAACGC 
               
               
                   
                   
                 ATAAGCGTCGCACGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 38 
                 Encoding Probe 28 
                 ATAGGAAATGGTGGTAGTGTGGACCAACAACGC 
               
               
                   
                   
                 ATAAGCGTCGGACATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 39 
                 Encoding Probe 29 
                 ATAGGAAATGGTGGTAGTGTAAGTCAGGAGACTT 
               
               
                   
                   
                 TAAGTCTCACCCATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 40 
                 Encoding Probe 30 
                 ATAGGAAATGGTGGTAGTGTTTGGGATTACGGGT 
               
               
                   
                   
                 CTACGTTTCTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 41 
                 Encoding Probe 31 
                 ATAGGAAATGGTGGTAGTGTAGGCAGGAGACTTT 
               
               
                   
                   
                 AAGTCTCAGCCTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 42 
                 Encoding Probe 32 
                 ATAGGAAATGGTGGTAGTGTGGAAGGAGACTTT 
               
               
                   
                   
                 AAGTCTCAGGCTCATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 43 
                 Encoding Probe 33 
                 ATAGGAAATGGTGGTAGTGTATGAACAACGCAT 
               
               
                   
                   
                 AAGCGTCGCACGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 44 
                 Encoding Probe 34 
                 ATAGGAAATGGTGGTAGTGTGGACCAACAACGC 
               
               
                   
                   
                 ATAAGCGTCCGAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 45 
                 Encoding Probe 35 
                 ATAGGAAATGGTGGTAGTGTGATCAACAACGCAT 
               
               
                   
                   
                 AAGCGTCGGACATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 46 
                 Encoding Probe 36 
                 ATAGGAAATGGTGGTAGTGTAGGCAGGAGACTTT 
               
               
                   
                   
                 AAGTCTCACCCATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 47 
                 Encoding Probe 37 
                 ATAGGAAATGGTGGTAGTGTGAAGGAGACTTTA 
               
               
                   
                   
                 AGTCTCAGGCTCATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 48 
                 Encoding Probe 38 
                 ATAGGAAATGGTGGTAGTGTAAGGAGACTTTAA 
               
               
                   
                   
                 GTCTCAGGGTCTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 49 
                 Encoding Probe 39 
                 ATAGGAAATGGTGGTAGTGTTGTTCAGCGTTAAA 
               
               
                   
                   
                 AGGTACCGCTAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 50 
                 Encoding Probe 40 
                 ATAGGAAATGGTGGTAGTGTTGGACAACGCATA 
               
               
                   
                   
                 AGCGTCGCACGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 51 
                 Encoding Probe 41 
                 ATAGGAAATGGTGGTAGTGTGATCAACAACGCAT 
               
               
                   
                   
                 AAGCGTCCGAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 52 
                 Encoding Probe 42 
                 ATAGGAAATGGTGGTAGTGTATGAACAACGCAT 
               
               
                   
                   
                 AAGCGTCGGACATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 53 
                 Encoding Probe 43 
                 ATAGGAAATGGTGGTAGTGTGGACCAACAACGC 
               
               
                   
                   
                 ATAAGCGTGCGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 54 
                 Encoding Probe 44 
                 ATAGGAAATGGTGGTAGTGTTGTTCAGCGTTAAA 
               
               
                   
                   
                 AGGTACCCCTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 55 
                 Encoding Probe 45 
                 ATAGGAAATGGTGGTAGTGTGTGCAGCGTTAAAA 
               
               
                   
                   
                 GGTACCGCTAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 56 
                 Encoding Probe 46 
                 AGAGTGAGTAGTAGTGGAGTGTGCTCAGTGTTAA 
               
               
                   
                   
                 AGTGCACCCCTAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 57 
                 Encoding Probe 47 
                 AGAGTGAGTAGTAGTGGAGTCCGCTCTGCCAAGT 
               
               
                   
                   
                 TCTGTGGTACAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 58 
                 Encoding Probe 48 
                 AGAGTGAGTAGTAGTGGAGTGAGTGTTAAAGTG 
               
               
                   
                   
                 CACCGGATTACGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 59 
                 Encoding Probe 49 
                 AGAGTGAGTAGTAGTGGAGTCATCAGCTAACGAT 
               
               
                   
                   
                 AGTGTGACCTCAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 60 
                 Encoding Probe 50 
                 AGAGTGAGTAGTAGTGGAGTTCTTTCTCCGCGAG 
               
               
                   
                   
                 GATAACCGGTAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 61 
                 Encoding Probe 51 
                 AGAGTGAGTAGTAGTGGAGTTTCCTTCTCCGCGA 
               
               
                   
                   
                 GGATAACCCCTAGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 62 
                 Encoding Probe 52 
                 AGAGTGAGTAGTAGTGGAGTGTCCCATGGGTAA 
               
               
                   
                   
                 ACCACTTCTGGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 63 
                 Encoding Probe 53 
                 AGAGTGAGTAGTAGTGGAGTAGTACGCCTCAGTG 
               
               
                   
                   
                 TTAAAGTCGTAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 64 
                 Encoding Probe 54 
                 AGAGTGAGTAGTAGTGGAGTGTGCTCAGTGTTAA 
               
               
                   
                   
                 AGTGCACGCCAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 65 
                 Encoding Probe 55 
                 AGAGTGAGTAGTAGTGGAGTCCGCAAGGCATCTC 
               
               
                   
                   
                 TGCCAAGAAGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 66 
                 Encoding Probe 56 
                 AGAGTGAGTAGTAGTGGAGTAGTGTTAAAGTGC 
               
               
                   
                   
                 ACCGGATTACGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 67 
                 Encoding Probe 57 
                 AGAGTGAGTAGTAGTGGAGTATAAGCTAACGAT 
               
               
                   
                   
                 AGTGTGACCTCAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 68 
                 Encoding Probe 58 
                 AGAGTGAGTAGTAGTGGAGTCTCTCTCCGCGAGG 
               
               
                   
                   
                 ATAACCCGTAAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 69 
                 Encoding Probe 59 
                 AGAGTGAGTAGTAGTGGAGTTCGCTCCGCGAGG 
               
               
                   
                   
                 ATAACCCCTAGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 70 
                 Encoding Probe 60 
                 AGAGTGAGTAGTAGTGGAGTAGTTCCATGGGTAA 
               
               
                   
                   
                 ACCACTTGTGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 71 
                 Encoding Probe 61 
                 AGAGTGAGTAGTAGTGGAGTGTACGCCTCAGTGT 
               
               
                   
                   
                 TAAAGTGGTGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 72 
                 Encoding Probe 62 
                 AGAGTGAGTAGTAGTGGAGTTGCTCAGTGTTAAA 
               
               
                   
                   
                 GTGCACCCCTAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 73 
                 Encoding Probe 63 
                 AGAGTGAGTAGTAGTGGAGTGTACGCCTCAGTGT 
               
               
                   
                   
                 TAAAGTGCTGGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 74 
                 Encoding Probe 64 
                 AGAGTGAGTAGTAGTGGAGTGAGTGTTAAAGTG 
               
               
                   
                   
                 CACCGGATAACAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 75 
                 Encoding Probe 65 
                 AGAGTGAGTAGTAGTGGAGTCGGAGTGTTAAAG 
               
               
                   
                   
                 TGCACCGGATTTGGGAAGAGTGAGTAGTAGTGG 
               
               
                   
                   
                 AGT 
               
               
                   
               
               
                 76 
                 Encoding Probe 66 
                 AGAGTGAGTAGTAGTGGAGTCATCAGCTAACGAT 
               
               
                   
                   
                 AGTGTGAGCTAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 77 
                 Encoding Probe 67 
                 AGAGTGAGTAGTAGTGGAGTAGTACGCCTCAGTG 
               
               
                   
                   
                 TTAAAGTGGTGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 78 
                 Encoding Probe 68 
                 AGAGTGAGTAGTAGTGGAGTAGTTCCATGGGTAA 
               
               
                   
                   
                 ACCACTTCTGGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 79 
                 Encoding Probe 69 
                 AGAGTGAGTAGTAGTGGAGTCGATCCGCGAGGA 
               
               
                   
                   
                 TAACCCCAAGTAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 80 
                 Encoding Probe 70 
                 AGAGTGAGTAGTAGTGGAGTTTCCTTCTCCGCGA 
               
               
                   
                   
                 GGATAACAGGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 81 
                 Encoding Probe 71 
                 TGTGATGGAAGTTAGAGGGTGAGGCTCAGTAGTT 
               
               
                   
                   
                 TTGGATGCTCATGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 82 
                 Encoding Probe 72 
                 TGTGATGGAAGTTAGAGGGTAGACGCGTCACTTA 
               
               
                   
                   
                 CGTGACACGGCTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 83 
                 Encoding Probe 73 
                 TGTGATGGAAGTTAGAGGGTGTGGAGGTGCTGGT 
               
               
                   
                   
                 AACTAAGCTGTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 84 
                 Encoding Probe 74 
                 TGTGATGGAAGTTAGAGGGTCTAGTTTTATGGGA 
               
               
                   
                   
                 TTAGCTCCAGGATGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 85 
                 Encoding Probe 75 
                 TGTGATGGAAGTTAGAGGGTGAGGAAAGTTCTCA 
               
               
                   
                   
                 GCATGTCTTCTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 86 
                 Encoding Probe 76 
                 TGTGATGGAAGTTAGAGGGTACACCCATGCTCGG 
               
               
                   
                   
                 CACTTCTCCCTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 87 
                 Encoding Probe 77 
                 TGTGATGGAAGTTAGAGGGTCGCGGTGTTTTTCA 
               
               
                   
                   
                 CACCCATACATGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 88 
                 Encoding Probe 78 
                 TGTGATGGAAGTTAGAGGGTTGGCCAGAGTGATA 
               
               
                   
                   
                 CATGAGGGCGTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 89 
                 Encoding Probe 79 
                 TGTGATGGAAGTTAGAGGGTTGGCTATCTCCGAG 
               
               
                   
                   
                 CTTGATTTCGTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 90 
                 Encoding Probe 80 
                 TGTGATGGAAGTTAGAGGGTGGCACACAGGAAA 
               
               
                   
                   
                 TTCCACCAAGGTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 91 
                 Encoding Probe 81 
                 TGTGATGGAAGTTAGAGGGTAAGATCCAACTTGC 
               
               
                   
                   
                 TGAACCAGGATGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 92 
                 Encoding Probe 82 
                 TGTGATGGAAGTTAGAGGGTTGCGTCACCTAACA 
               
               
                   
                   
                 AGTAGGCAGGTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 93 
                 Encoding Probe 83 
                 TGTGATGGAAGTTAGAGGGTCGTGTATTAACTTA 
               
               
                   
                   
                 CTGCCCTTCGAGTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 94 
                 Encoding Probe 84 
                 TGTGATGGAAGTTAGAGGGTACAAGACAAAGTTT 
               
               
                   
                   
                 CTCGTGCAGGTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 95 
                 Encoding Probe 85 
                 TGTGATGGAAGTTAGAGGGTAAACTTCAAAGATC 
               
               
                   
                   
                 CTTTCGCCATTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 96 
                 Encoding Probe 86 
                 TGTGATGGAAGTTAGAGGGTGCACGCTAAAATCA 
               
               
                   
                   
                 ATGAAGCTATTTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 97 
                 Encoding Probe 87 
                 TGTGATGGAAGTTAGAGGGTCGATCTGATAGCGT 
               
               
                   
                   
                 GAGGTCCCTTTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 98 
                 Encoding Probe 88 
                 TGTGATGGAAGTTAGAGGGTATAATTCAGTACAA 
               
               
                   
                   
                 GATACCTAGGAATTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 99 
                 Encoding Probe 89 
                 TGTGATGGAAGTTAGAGGGTAGGCGCTGAATCCA 
               
               
                   
                   
                 GGAGCAACGATGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 100 
                 Encoding Probe 90 
                 TGTGATGGAAGTTAGAGGGTCAAAACGCTCTATG 
               
               
                   
                   
                 ATCGTCAATATGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 101 
                 Encoding Probe 91 
                 TGTGATGGAAGTTAGAGGGTGCAGTGTTTTTCAC 
               
               
                   
                   
                 ACCCATTGTGCATGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 102 
                 Encoding Probe 92 
                 TGTGATGGAAGTTAGAGGGTCTGCGATCGGTTTT 
               
               
                   
                   
                 ATGGGATATCTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 103 
                 Encoding Probe 93 
                 TGTGATGGAAGTTAGAGGGTGGATCGACGTGTCT 
               
               
                   
                   
                 GTCTCGCTCATGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 104 
                 Encoding Probe 94 
                 TGTGATGGAAGTTAGAGGGTGGTGCAGTAACCA 
               
               
                   
                   
                 GAAGTACACCTTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 105 
                 Encoding Probe 95 
                 TGTGATGGAAGTTAGAGGGTAGTTCCAACTTGCT 
               
               
                   
                   
                 GAACCACGATTGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 106 
                 Encoding Probe 96 
                 TGAAAGGAATGGGTTGTGGTAAAGAGATTAGCTT 
               
               
                   
                   
                 AGCCTCGGCTTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 107 
                 Encoding Probe 97 
                 TGAAAGGAATGGGTTGTGGTGTCCTCACGATCTG 
               
               
                   
                   
                 CCTTCGAGCGTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 108 
                 Encoding Probe 98 
                 TGAAAGGAATGGGTTGTGGTTTAACCTAAAGGTG 
               
               
                   
                   
                 TACTCCAGTCTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 109 
                 Encoding Probe 99 
                 TGAAAGGAATGGGTTGTGGTTCGTTGACTCCTCT 
               
               
                   
                   
                 TCAGACTATGTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 110 
                 Encoding Probe 100 
                 TGAAAGGAATGGGTTGTGGTTGAGGCTGATCGTA 
               
               
                   
                   
                 TGATCAGGTGTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 111 
                 Encoding Probe 101 
                 TGAAAGGAATGGGTTGTGGTTCTAGCTTAGCCTC 
               
               
                   
                   
                 GCGACTTGCGTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 112 
                 Encoding Probe 102 
                 TGAAAGGAATGGGTTGTGGTACTTTTCCAAGTCA 
               
               
                   
                   
                 TTCGACTATGACTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 113 
                 Encoding Probe 103 
                 TGAAAGGAATGGGTTGTGGTGATGGGTTTTTACC 
               
               
                   
                   
                 CTCTTTGACACTTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 114 
                 Encoding Probe 104 
                 TGAAAGGAATGGGTTGTGGTTGATCCAAGTCATT 
               
               
                   
                   
                 CGACTATCACTTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 115 
                 Encoding Probe 105 
                 TGAAAGGAATGGGTTGTGGTATGTCAAGGGATG 
               
               
                   
                   
                 AACAGTTACAGATGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 116 
                 Encoding Probe 106 
                 TGAAAGGAATGGGTTGTGGTGCAAGGGATGAAC 
               
               
                   
                   
                 AGTTACTCTGTATGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 117 
                 Encoding Probe 107 
                 TGAAAGGAATGGGTTGTGGTAGGCTAGGTGTCTT 
               
               
                   
                   
                 CCACATTTGCATGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 118 
                 Encoding Probe 108 
                 TGAAAGGAATGGGTTGTGGTAGTCGACTATCTGA 
               
               
                   
                   
                 AAGAACTACCTATTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 119 
                 Encoding Probe 109 
                 TGAAAGGAATGGGTTGTGGTGTAGGTTTTGATTG 
               
               
                   
                   
                 TTATACGGTATATCTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 120 
                 Encoding Probe 110 
                 TGAAAGGAATGGGTTGTGGTATTTCCTATTAAAG 
               
               
                   
                   
                 ATGTTGGGTAGGTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 121 
                 Encoding Probe 111 
                 TGAAAGGAATGGGTTGTGGTCGTCTCCGGTGGAA 
               
               
                   
                   
                 AAAGAAGGCATGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 122 
                 Encoding Probe 112 
                 TGAAAGGAATGGGTTGTGGTCAAGCATGGTTACA 
               
               
                   
                   
                 GGTGTATGGATGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 123 
                 Encoding Probe 113 
                 TGAAAGGAATGGGTTGTGGTTATCCTAAAGGTGT 
               
               
                   
                   
                 ACTCCACTCGTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 124 
                 Encoding Probe 114 
                 TGAAAGGAATGGGTTGTGGTGGACACAGCTTGTC 
               
               
                   
                   
                 CTTAAGATTTTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 125 
                 Encoding Probe 115 
                 TGAAAGGAATGGGTTGTGGTTGTTCCGATCGTCT 
               
               
                   
                   
                 GCATTCCAATTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 126 
                 Encoding Probe 116 
                 TGAAAGGAATGGGTTGTGGTTCGGTCCTTAAGAA 
               
               
                   
                   
                 AAGAAGCATAACTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 127 
                 Encoding Probe 117 
                 TGAAAGGAATGGGTTGTGGTCGAGACAGACATTT 
               
               
                   
                   
                 CCGATCGAGATGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 128 
                 Encoding Probe 118 
                 TGAAAGGAATGGGTTGTGGTGACGCTGATCGTAT 
               
               
                   
                   
                 GATCAGCTGGTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 129 
                 Encoding Probe 119 
                 TGAAAGGAATGGGTTGTGGTGCCCGGGCCGCTGT 
               
               
                   
                   
                 TTTCTCAGATTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 130 
                 Encoding Probe 120 
                 TGAAAGGAATGGGTTGTGGTCTTTCGGTACTATT 
               
               
                   
                   
                 ATTTCCCTCAGGTGAAAGGAATGGGTTGTGGT 
               
               
                   
               
               
                 131 
                 Encoding Probe 121 
                 TTGGAGGTGTAGGGAGTAAACCCACGATTGTTGG 
               
               
                   
                   
                 TAACCTGATCGTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 132 
                 Encoding Probe 122 
                 TTGGAGGTGTAGGGAGTAAATGGAGCATTAAGT 
               
               
                   
                   
                 GACCGGATAACTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 133 
                 Encoding Probe 123 
                 TTGGAGGTGTAGGGAGTAAACTAGCCTAATCACT 
               
               
                   
                   
                 CTGCCTAGTATTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 134 
                 Encoding Probe 124 
                 TTGGAGGTGTAGGGAGTAAAGGTGCAGTTACCAC 
               
               
                   
                   
                 CAGTACGGCTTTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 135 
                 Encoding Probe 125 
                 TTGGAGGTGTAGGGAGTAAAAGCGCTACAACGTT 
               
               
                   
                   
                 TCACTTCACTTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 136 
                 Encoding Probe 126 
                 TTGGAGGTGTAGGGAGTAAAGACGTCCACATTTC 
               
               
                   
                   
                 ATAGTCTCCCTGGTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 137 
                 Encoding Probe 127 
                 TTGGAGGTGTAGGGAGTAAAAAAGTCACTCAAG 
               
               
                   
                   
                 GTGACAGGGTTCTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 138 
                 Encoding Probe 128 
                 TTGGAGGTGTAGGGAGTAAAGAACCACCTTAGTG 
               
               
                   
                   
                 GTTCGTCTAGTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 139 
                 Encoding Probe 129 
                 TTGGAGGTGTAGGGAGTAAACAAGGGTACGATT 
               
               
                   
                   
                 GTTGGTAAGGATTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 140 
                 Encoding Probe 130 
                 TTGGAGGTGTAGGGAGTAAAAAGTTAGGTCACTC 
               
               
                   
                   
                 AAGGTGACAGGGTTCTTGGAGGTGTAGGGAGTA 
               
               
                   
                   
                 AA 
               
               
                   
               
               
                 141 
                 Encoding Probe 131 
                 TTGGAGGTGTAGGGAGTAAAGAAGGTCACTCAA 
               
               
                   
                   
                 GGTGACAGGGAAGTGATTGGAGGTGTAGGGAGT 
               
               
                   
                   
                 AAA 
               
               
                   
               
               
                 142 
                 Encoding Probe 132 
                 TTGGAGGTGTAGGGAGTAAAGAAAGTTCCCGCC 
               
               
                   
                   
                 ATCACGCGGACTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 143 
                 Encoding Probe 133 
                 TTGGAGGTGTAGGGAGTAAAGTTGACTTCACTTA 
               
               
                   
                   
                 CCGCCAGGCATTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 144 
                 Encoding Probe 134 
                 TTGGAGGTGTAGGGAGTAAAGAGGCGCCTGAGT 
               
               
                   
                   
                 ATTCTCTAGGATTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 145 
                 Encoding Probe 135 
                 TTGGAGGTGTAGGGAGTAAATAACCTAATCACTC 
               
               
                   
                   
                 TGCCTACAACGTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 146 
                 Encoding Probe 136 
                 TTGGAGGTGTAGGGAGTAAACCTTGCCTAATCAC 
               
               
                   
                   
                 TCTGCCTTGTTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 147 
                 Encoding Probe 137 
                 TTGGAGGTGTAGGGAGTAAACCTTGCCTAATCAC 
               
               
                   
                   
                 TCTGCCTACTACTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 148 
                 Encoding Probe 138 
                 TTGGAGGTGTAGGGAGTAAAGGAATCGCAGTTA 
               
               
                   
                   
                 CCACCAGTACCCCTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 149 
                 Encoding Probe 139 
                 TTGGAGGTGTAGGGAGTAAACGAGGCTCCGTCCG 
               
               
                   
                   
                 CAAGGGAGAATTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 150 
                 Encoding Probe 140 
                 TTGGAGGTGTAGGGAGTAAACGTGGACTTCACTT 
               
               
                   
                   
                 ACCGCCAGGCATTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 151 
                 Encoding Probe 141 
                 TTGGAGGTGTAGGGAGTAAACCCACGATTGTTGG 
               
               
                   
                   
                 TAACCTGTTCTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 152 
                 Encoding Probe 142 
                 TTGGAGGTGTAGGGAGTAAAGTGCAGCATTAAGT 
               
               
                   
                   
                 GACCGGAAAATTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 153 
                 Encoding Probe 143 
                 TTGGAGGTGTAGGGAGTAAATAACCTAATCACTC 
               
               
                   
                   
                 TGCCTACTACTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 154 
                 Encoding Probe 144 
                 TTGGAGGTGTAGGGAGTAAAGTACAGTTACCACC 
               
               
                   
                   
                 AGTACGGGTTATTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 155 
                 Encoding Probe 145 
                 TTGGAGGTGTAGGGAGTAAATAGCGCTACAACGT 
               
               
                   
                   
                 TTCACTTGACTTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 156 
                 Encoding Probe 146 
                 AGGGTGTGTTTGTAAAGGGTCGTACGCAAAGCGA 
               
               
                   
                   
                 AACGCTTACCAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 157 
                 Encoding Probe 147 
                 AGGGTGTGTTTGTAAAGGGTCCATTAACCTCACT 
               
               
                   
                   
                 CCCTTCCAGGAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 158 
                 Encoding Probe 148 
                 AGGGTGTGTTTGTAAAGGGTCAGTCATGCTGTCG 
               
               
                   
                   
                 TTACGCATAAAAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 159 
                 Encoding Probe 149 
                 AGGGTGTGTTTGTAAAGGGTGTACCGGCTGTAAC 
               
               
                   
                   
                 GGTTCATTAGAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 160 
                 Encoding Probe 150 
                 AGGGTGTGTTTGTAAAGGGTAAGGACCCTTAAAG 
               
               
                   
                   
                 GGTCAGGCTCAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 161 
                 Encoding Probe 151 
                 AGGGTGTGTTTGTAAAGGGTGAAGGACCCTTAAA 
               
               
                   
                   
                 GGGTCAGCCTAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 162 
                 Encoding Probe 152 
                 AGGGTGTGTTTGTAAAGGGTGTGCAGCGTTAGTA 
               
               
                   
                   
                 ACGTTCCCCTAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 163 
                 Encoding Probe 153 
                 AGGGTGTGTTTGTAAAGGGTTTCGCTTCACCTAC 
               
               
                   
                   
                 CATCAGCCACAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 164 
                 Encoding Probe 154 
                 AGGGTGTGTTTGTAAAGGGTGTCTTAGTAACGTT 
               
               
                   
                   
                 CCGGATTTTGGAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 165 
                 Encoding Probe 155 
                 AGGGTGTGTTTGTAAAGGGTGCAGTAACGTTCCG 
               
               
                   
                   
                 GATTTACGACAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 166 
                 Encoding Probe 156 
                 AGGGTGTGTTTGTAAAGGGTGCGTTTGCGCACCA 
               
               
                   
                   
                 CGCAAAGGCTAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 167 
                 Encoding Probe 157 
                 AGGGTGTGTTTGTAAAGGGTCAACCGTCCATCAT 
               
               
                   
                   
                 GCTGTCGTATGAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 168 
                 Encoding Probe 158 
                 AGGGTGTGTTTGTAAAGGGTCGACGTTACGCATT 
               
               
                   
                   
                 TTGCGCAGGTAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 169 
                 Encoding Probe 159 
                 AGGGTGTGTTTGTAAAGGGTAGTCCTCAGCGTTA 
               
               
                   
                   
                 GTAACGTTCGCCAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 170 
                 Encoding Probe 160 
                 AGGGTGTGTTTGTAAAGGGTAACTTCCCGACCGA 
               
               
                   
                   
                 ATCGCTGCGTAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 171 
                 Encoding Probe 161 
                 AGGGTGTGTTTGTAAAGGGTACTCTCCGTTAACC 
               
               
                   
                   
                 GTCCATCTACAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 172 
                 Encoding Probe 162 
                 AGGGTGTGTTTGTAAAGGGTGGCAACCGTCCATC 
               
               
                   
                   
                 ATGCTGTGCAAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 173 
                 Encoding Probe 163 
                 AGGGTGTGTTTGTAAAGGGTGGTCCAAAACGCTC 
               
               
                   
                   
                 CACTGCCACTAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 174 
                 Encoding Probe 164 
                 AGGGTGTGTTTGTAAAGGGTGTATGCTGTCGTTA 
               
               
                   
                   
                 CGCATTTTCGCAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 175 
                 Encoding Probe 165 
                 AGGGTGTGTTTGTAAAGGGTGCAACCGTCCATCA 
               
               
                   
                   
                 TGCTGTCCAAAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 176 
                 Encoding Probe 166 
                 AGGGTGTGTTTGTAAAGGGTCGAACTGCCTGATT 
               
               
                   
                   
                 TTTGACGAACAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 177 
                 Encoding Probe 167 
                 AGGGTGTGTTTGTAAAGGGTGCGCACGCAAAGC 
               
               
                   
                   
                 GAAACGCTTTCCAAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 178 
                 Encoding Probe 168 
                 AGGGTGTGTTTGTAAAGGGTCATGTCAATGAATA 
               
               
                   
                   
                 AGGTTATTAACCAGTAGGGTGTGTTTGTAAAGGG 
               
               
                   
                   
                 T 
               
               
                   
               
               
                 179 
                 Encoding Probe 169 
                 AGGGTGTGTTTGTAAAGGGTCAGTCATGCTGTCG 
               
               
                   
                   
                 TTACGCAAAAAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 180 
                 Encoding Probe 170 
                 AGGGTGTGTTTGTAAAGGGTTGTGCCGGCTGTAA 
               
               
                   
                   
                 CGGTTCAATAAGGGTGTGTTTGTAAAGGGT 
               
               
                   
               
               
                 181 
                 Encoding Probe 171 
                 AGGTTAGGTTGAGAATAGGATGGGTCGCTTAAAG 
               
               
                   
                   
                 CGACAGGATAAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 182 
                 Encoding Probe 172 
                 AGGTTAGGTTGAGAATAGGAGAAGTCCGTAGAC 
               
               
                   
                   
                 ATTATGCGCATAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 183 
                 Encoding Probe 173 
                 AGGTTAGGTTGAGAATAGGATGCCCCCGACCCAG 
               
               
                   
                   
                 TTTATGGCGGAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 184 
                 Encoding Probe 174 
                 AGGTTAGGTTGAGAATAGGAGAAGGTCGCTTAA 
               
               
                   
                   
                 AGCGACAGGCTTAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 185 
                 Encoding Probe 175 
                 AGGTTAGGTTGAGAATAGGAAAGTTAGGTCGCTT 
               
               
                   
                   
                 AAAGCGACTCCAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 186 
                 Encoding Probe 176 
                 AGGTTAGGTTGAGAATAGGAGCTCAGTTTATGGG 
               
               
                   
                   
                 CCTAGGTATCAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 187 
                 Encoding Probe 177 
                 AGGTTAGGTTGAGAATAGGATCCGCTTAAAGCGA 
               
               
                   
                   
                 CAGGGAACTGAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 188 
                 Encoding Probe 178 
                 AGGTTAGGTTGAGAATAGGAAAGTTAGGTCGCTT 
               
               
                   
                   
                 AAAGCGACAGGGTTCAGGTTAGGTTGAGAATAG 
               
               
                   
                   
                 GA 
               
               
                   
               
               
                 189 
                 Encoding Probe 179 
                 AGGTTAGGTTGAGAATAGGAGGAAGGTCGCTTA 
               
               
                   
                   
                 AAGCGACAGGGAACTGAGGTTAGGTTGAGAATA 
               
               
                   
                   
                 GGA 
               
               
                   
               
               
                 190 
                 Encoding Probe 180 
                 AGGTTAGGTTGAGAATAGGAGGAAGGTCGCTTA 
               
               
                   
                   
                 AAGCGACACCCAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 191 
                 Encoding Probe 181 
                 AGGTTAGGTTGAGAATAGGAAAAGTTCCCACCAT 
               
               
                   
                   
                 TACGTGCACCAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 192 
                 Encoding Probe 182 
                 AGGTTAGGTTGAGAATAGGACAATTTAGGTCGCT 
               
               
                   
                   
                 TAAAGCGACAGGCTTAGGTTAGGTTGAGAATAG 
               
               
                   
                   
                 GA 
               
               
                   
               
               
                 193 
                 Encoding Probe 183 
                 AGGTTAGGTTGAGAATAGGACTGAGTTTATGGGC 
               
               
                   
                   
                 CTAGGTTACTTAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 194 
                 Encoding Probe 184 
                 AGGTTAGGTTGAGAATAGGAGGCCCAGTTTATGG 
               
               
                   
                   
                 GCCTAGGAATAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 195 
                 Encoding Probe 185 
                 AGGTTAGGTTGAGAATAGGAGAAGGTCGCTTAA 
               
               
                   
                   
                 AGCGACAGCCTAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 196 
                 Encoding Probe 186 
                 AGGTTAGGTTGAGAATAGGACCACTTAAAGCGA 
               
               
                   
                   
                 CAGGGAAGTGAAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 197 
                 Encoding Probe 187 
                 AGGTTAGGTTGAGAATAGGAACTTCCCACCATTA 
               
               
                   
                   
                 CGTGCTGCGTAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 198 
                 Encoding Probe 188 
                 AGGTTAGGTTGAGAATAGGAGAAGGTCGCTTAA 
               
               
                   
                   
                 AGCGACAGGGAAGTGAAGGTTAGGTTGAGAATA 
               
               
                   
                   
                 GGA 
               
               
                   
               
               
                 199 
                 Encoding Probe 189 
                 AGGTTAGGTTGAGAATAGGAAATTCGCTTAAAGC 
               
               
                   
                   
                 GACAGGGTTCAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 200 
                 Encoding Probe 190 
                 AGGTTAGGTTGAGAATAGGAAACTTCCCACCATT 
               
               
                   
                   
                 ACGTGCTCCGAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 201 
                 Encoding Probe 191 
                 AGGTTAGGTTGAGAATAGGAGGAACCCAGTTTAT 
               
               
                   
                   
                 GGGCCTACCAAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 202 
                 Encoding Probe 192 
                 AGGTTAGGTTGAGAATAGGAAAAGTCGCTTAAA 
               
               
                   
                   
                 GCGACAGGGTTCAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 203 
                 Encoding Probe 193 
                 AGGTTAGGTTGAGAATAGGACAGCGACCCAGTTT 
               
               
                   
                   
                 ATGGGCCTAGCAAAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 204 
                 Encoding Probe 194 
                 AGGTTAGGTTGAGAATAGGACTGAGTTTATGGGC 
               
               
                   
                   
                 CTAGGTTTCTAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 205 
                 Encoding Probe 195 
                 AGGTTAGGTTGAGAATAGGAGCTCAGTTTATGGG 
               
               
                   
                   
                 CCTAGGTTACTTAGGTTAGGTTGAGAATAGGA 
               
               
                   
               
               
                 206 
                 Encoding Probe 196 
                 TAGAGTTGATAGAGGGAGAACGAATGAGTAAAT 
               
               
                   
                   
                 CACTTCACCTAGTATAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 207 
                 Encoding Probe 197 
                 TAGAGTTGATAGAGGGAGAAGGATTCGCTTCATT 
               
               
                   
                   
                 ACGCTATGTAAAGTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 208 
                 Encoding Probe 198 
                 TAGAGTTGATAGAGGGAGAATGTTCAGCGTTAAA 
               
               
                   
                   
                 AAGGTACCGCTATAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 209 
                 Encoding Probe 199 
                 TAGAGTTGATAGAGGGAGAACCAGCTTCATTACG 
               
               
                   
                   
                 CTATGTATTGTGTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 210 
                 Encoding Probe 200 
                 TAGAGTTGATAGAGGGAGAATGTTCAGCGTTAAA 
               
               
                   
                   
                 AAGGTACCCCTTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 211 
                 Encoding Probe 201 
                 TAGAGTTGATAGAGGGAGAAGCCCGCTTCATTAC 
               
               
                   
                   
                 GCTATGTAAAGTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 212 
                 Encoding Probe 202 
                 TAGAGTTGATAGAGGGAGAAGCGCCCGGGTAAC 
               
               
                   
                   
                 GGGTCCACGAATTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 213 
                 Encoding Probe 203 
                 TAGAGTTGATAGAGGGAGAAGTGCAGCGTTAAA 
               
               
                   
                   
                 AAGGTACCGCTATAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 214 
                 Encoding Probe 204 
                 TAGAGTTGATAGAGGGAGAAGGATTCGCTTCATT 
               
               
                   
                   
                 ACGCTATGATATAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 215 
                 Encoding Probe 205 
                 TAGAGTTGATAGAGGGAGAAGCGCCCGGGTAAC 
               
               
                   
                   
                 GGGTCCACCAATAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 216 
                 Encoding Probe 206 
                 TAGAGTTGATAGAGGGAGAATGGAGCGTTAAAA 
               
               
                   
                   
                 AGGTACCGCTATAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 217 
                 Encoding Probe 207 
                 TAGAGTTGATAGAGGGAGAAGGAGTTCGCTTCAT 
               
               
                   
                   
                 TACGCTATGTAAAGTAGAGTTGATAGAGGGAGA 
               
               
                   
                   
                 A 
               
               
                   
               
               
                 218 
                 Encoding Probe 208 
                 TAGAGTTGATAGAGGGAGAAGGCTCGCTTCATTA 
               
               
                   
                   
                 CGCTATGTATAGTTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 219 
                 Encoding Probe 209 
                 TAGAGTTGATAGAGGGAGAACGTCCGGGTAACG 
               
               
                   
                   
                 GGTCCACGAATTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 220 
                 Encoding Probe 210 
                 TAGAGTTGATAGAGGGAGAAAATTCAATGTATCG 
               
               
                   
                   
                 CTACACTTTGTTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 221 
                 Encoding Probe 211 
                 TAGAGTTGATAGAGGGAGAACGAATGAGTAAAT 
               
               
                   
                   
                 CACTTCACCTTGTTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 222 
                 Encoding Probe 212 
                 TAGAGTTGATAGAGGGAGAAGTGCAGCGTTAAA 
               
               
                   
                   
                 AAGGTACCCCTTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 223 
                 Encoding Probe 213 
                 TAGAGTTGATAGAGGGAGAAGGCTCGCTTCATTA 
               
               
                   
                   
                 CGCTATGTTAATAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 224 
                 Encoding Probe 214 
                 TAGAGTTGATAGAGGGAGAAGAGGCATACCTCA 
               
               
                   
                   
                 CGATACACGAATAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 225 
                 Encoding Probe 215 
                 TAGAGTTGATAGAGGGAGAAGGAGTTCGCTTCAT 
               
               
                   
                   
                 TACGCTATGTTAATAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 226 
                 Encoding Probe 216 
                 TAGAGTTGATAGAGGGAGAAGGATTCGCTTCATT 
               
               
                   
                   
                 ACGCTATGTATTGTGTAGAGTTGATAGAGGGAGA 
               
               
                   
                   
                 A 
               
               
                   
               
               
                 227 
                 Encoding Probe 217 
                 TAGAGTTGATAGAGGGAGAAGGAGTTCGCTTCAT 
               
               
                   
                   
                 TACGCTATCATTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 228 
                 Encoding Probe 218 
                 TAGAGTTGATAGAGGGAGAAGCCCGCTTCATTAC 
               
               
                   
                   
                 GCTATGTATTGTGTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 229 
                 Encoding Probe 219 
                 TAGAGTTGATAGAGGGAGAAGGCTCGCTTCATTA 
               
               
                   
                   
                 CGCTATGTATTGTGTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 230 
                 Encoding Probe 220 
                 TAGAGTTGATAGAGGGAGAAGGCTCGCTTCATTA 
               
               
                   
                   
                 CGCTATGTAAAGTAGAGTTGATAGAGGGAGAA 
               
               
                   
               
               
                 231 
                 Encoding Probe 221 
                 GATGATGTAGTAGTAAGGGTACCTCTTCGACTGG 
               
               
                   
                   
                 TCTCAGCAGGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 232 
                 Encoding Probe 222 
                 GATGATGTAGTAGTAAGGGTTGCAATCGATGAGG 
               
               
                   
                   
                 TTATTAACCTGTAGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 233 
                 Encoding Probe 223 
                 GATGATGTAGTAGTAAGGGTCATCAGTCACACCC 
               
               
                   
                   
                 GAAGGTGCTAGGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 234 
                 Encoding Probe 224 
                 GATGATGTAGTAGTAAGGGTGCAATCGATGAGGT 
               
               
                   
                   
                 TATTAACCTGTAGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 235 
                 Encoding Probe 225 
                 GATGATGTAGTAGTAAGGGTCATCAGTCACACCC 
               
               
                   
                   
                 GAAGGTGCAGGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 236 
                 Encoding Probe 226 
                 GATGATGTAGTAGTAAGGGTATGAGTCACACCCG 
               
               
                   
                   
                 AAGGTGCTAGGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 237 
                 Encoding Probe 227 
                 GATGATGTAGTAGTAAGGGTTCCCTTCACCTACA 
               
               
                   
                   
                 CACCAGCGACGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 238 
                 Encoding Probe 228 
                 GATGATGTAGTAGTAAGGGTTCCCTTCACCTACA 
               
               
                   
                   
                 CACCAGCCACGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 239 
                 Encoding Probe 229 
                 GATGATGTAGTAGTAAGGGTTGACCGCAACCCCG 
               
               
                   
                   
                 GTGAGGGCGGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 240 
                 Encoding Probe 230 
                 GATGATGTAGTAGTAAGGGTAGAGACTGGTCTCA 
               
               
                   
                   
                 GCTCCACGGCGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 241 
                 Encoding Probe 231 
                 GATGATGTAGTAGTAAGGGTATGAGTCACACCCG 
               
               
                   
                   
                 AAGGTGCAGGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 242 
                 Encoding Probe 232 
                 GATGATGTAGTAGTAAGGGTTGCGTCACACCCGA 
               
               
                   
                   
                 AGGTGCTAGGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 243 
                 Encoding Probe 233 
                 GATGATGTAGTAGTAAGGGTGTGCTCAGCCTTGA 
               
               
                   
                   
                 TTATCCGCTAGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 244 
                 Encoding Probe 234 
                 GATGATGTAGTAGTAAGGGTCCACGTCAATCGAT 
               
               
                   
                   
                 GAGGTTAAATGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 245 
                 Encoding Probe 235 
                 GATGATGTAGTAGTAAGGGTAATAACCTCATCGC 
               
               
                   
                   
                 CTTCCTCAGGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 246 
                 Encoding Probe 236 
                 GATGATGTAGTAGTAAGGGTCCCACGTCAATCGA 
               
               
                   
                   
                 TGAGGTTTAAGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 247 
                 Encoding Probe 237 
                 GATGATGTAGTAGTAAGGGTCATCAGTCACACCC 
               
               
                   
                   
                 GAAGGTGGAGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 248 
                 Encoding Probe 238 
                 GATGATGTAGTAGTAAGGGTCCCTTCACCTACAC 
               
               
                   
                   
                 ACCAGCGACGGATGATGTAGTAGTAAGGGT 
               
               
                   
               
               
                 249 
                 Encoding Probe 239 
                 ATAGGAAATGGTGGTAGTGTCTACCGACCGTGAT 
               
               
                   
                   
                 TAGCTAAGGATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 250 
                 Encoding Probe 240 
                 ATAGGAAATGGTGGTAGTGTCAACTGGAGCTTAG 
               
               
                   
                   
                 AGGATTTTGGATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 251 
                 Encoding Probe 241 
                 ATAGGAAATGGTGGTAGTGTCCCTTAAAGGCCCA 
               
               
                   
                   
                 GGGAAGAGAGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 252 
                 Encoding Probe 242 
                 ATAGGAAATGGTGGTAGTGTAAGCGCTTATCTTT 
               
               
                   
                   
                 TCCGCACAATTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 253 
                 Encoding Probe 243 
                 ATAGGAAATGGTGGTAGTGTCCCTTCACCTACAT 
               
               
                   
                   
                 GCCAGCGACGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 254 
                 Encoding Probe 244 
                 ATAGGAAATGGTGGTAGTGTCTGTGTCCTCACCC 
               
               
                   
                   
                 CAGATTAACCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 255 
                 Encoding Probe 245 
                 ATAGGAAATGGTGGTAGTGTATGTTTAATGTTAC 
               
               
                   
                   
                 CTGGAGCTATCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 256 
                 Encoding Probe 246 
                 ATAGGAAATGGTGGTAGTGTTCCATCAACTACTT 
               
               
                   
                   
                 CTGCACCGATCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 257 
                 Encoding Probe 247 
                 ATAGGAAATGGTGGTAGTGTGCGCAGGGTTGATA 
               
               
                   
                   
                 TGCAACCCCTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 258 
                 Encoding Probe 248 
                 ATAGGAAATGGTGGTAGTGTGATCAACAACGCTA 
               
               
                   
                   
                 AGCGTCGGACTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 259 
                 Encoding Probe 249 
                 ATAGGAAATGGTGGTAGTGTAGTTCCATCCGCGA 
               
               
                   
                   
                 GGGACTTGTGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 260 
                 Encoding Probe 250 
                 ATAGGAAATGGTGGTAGTGTTAATGAACGTATTA 
               
               
                   
                   
                 AGCTCACCTGGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 261 
                 Encoding Probe 251 
                 ATAGGAAATGGTGGTAGTGTGTCCATCAACTACT 
               
               
                   
                   
                 TCTGCACCGATCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 262 
                 Encoding Probe 252 
                 ATAGGAAATGGTGGTAGTGTATACCCTTTGCTGC 
               
               
                   
                   
                 GCGACTTAGGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 263 
                 Encoding Probe 253 
                 ATAGGAAATGGTGGTAGTGTCAGTACCTTGCAAC 
               
               
                   
                   
                 TAATCGCGGTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 264 
                 Encoding Probe 254 
                 ATAGGAAATGGTGGTAGTGTCAGTTGATGAACGT 
               
               
                   
                   
                 ATTAAGCTCAGGTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 265 
                 Encoding Probe 255 
                 ATAGGAAATGGTGGTAGTGTTATCAGACAGGATG 
               
               
                   
                   
                 TCACGTGAGGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 266 
                 Encoding Probe 256 
                 ATAGGAAATGGTGGTAGTGTGATCATCGAACTCA 
               
               
                   
                   
                 CGACCTGTCGTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 267 
                 Encoding Probe 257 
                 ATAGGAAATGGTGGTAGTGTTGTAGCCGATTCAG 
               
               
                   
                   
                 GTTCTGGCGATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 268 
                 Encoding Probe 258 
                 ATAGGAAATGGTGGTAGTGTATAATTCATGACAT 
               
               
                   
                   
                 GATAATGTGTGCTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 269 
                 Encoding Probe 259 
                 ATAGGAAATGGTGGTAGTGTATAGGCAGTGTCCT 
               
               
                   
                   
                 ACTCTCGGTATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 270 
                 Encoding Probe 260 
                 ATAGGAAATGGTGGTAGTGTAATGGGCCGAGTTA 
               
               
                   
                   
                 GAACATCTTTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 271 
                 Encoding Probe 261 
                 ATAGGAAATGGTGGTAGTGTAAAGCGCTTATCTT 
               
               
                   
                   
                 TTCCGCAGAATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 272 
                 Encoding Probe 262 
                 ATAGGAAATGGTGGTAGTGTAAAGGGCCTTAAA 
               
               
                   
                   
                 GGCCCAGGCTTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 273 
                 Encoding Probe 263 
                 ATAGGAAATGGTGGTAGTGTTCCCCTTAAAGGCC 
               
               
                   
                   
                 CAGGGAACTGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 274 
                 Encoding Probe 264 
                 AGAGTGAGTAGTAGTGGAGTCCCCGATTCCTGTG 
               
               
                   
                   
                 TAACTGAAGGAATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 275 
                 Encoding Probe 265 
                 AGAGTGAGTAGTAGTGGAGTGGACACGTATACA 
               
               
                   
                   
                 AAGTATACATCCCGTTGTGGAGGGATTGAAGGAT 
               
               
                   
                   
                 A 
               
               
                   
               
               
                 276 
                 Encoding Probe 266 
                 AGAGTGAGTAGTAGTGGAGTACGGCAAGTAAGG 
               
               
                   
                   
                 AAAAGGGTACGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 277 
                 Encoding Probe 267 
                 AGAGTGAGTAGTAGTGGAGTACGCACCTGTATCT 
               
               
                   
                   
                 AGATTCCCGTTCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 278 
                 Encoding Probe 268 
                 AGAGTGAGTAGTAGTGGAGTACCGTCTGGATTGT 
               
               
                   
                   
                 TTTCCTCTACTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 279 
                 Encoding Probe 269 
                 AGAGTGAGTAGTAGTGGAGTAGACGGATAGTAC 
               
               
                   
                   
                 TCATAGGTATTGCCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 280 
                 Encoding Probe 270 
                 AGAGTGAGTAGTAGTGGAGTGAAAGTTCCCATCC 
               
               
                   
                   
                 GAAATGCTGCGTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 281 
                 Encoding Probe 271 
                 AGAGTGAGTAGTAGTGGAGTCGAGCCACTAAAG 
               
               
                   
                   
                 CCTCAAAGGAGGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 282 
                 Encoding Probe 272 
                 AGAGTGAGTAGTAGTGGAGTGAGCGTCAGTATTA 
               
               
                   
                   
                 GGCCAGATGGGACTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 283 
                 Encoding Probe 273 
                 AGAGTGAGTAGTAGTGGAGTTGGGAATTCTACCA 
               
               
                   
                   
                 TCCTCTCCGTATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 284 
                 Encoding Probe 274 
                 AGAGTGAGTAGTAGTGGAGTGGTCTCTCCCATAC 
               
               
                   
                   
                 TCTAGCTGTGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 285 
                 Encoding Probe 275 
                 AGAGTGAGTAGTAGTGGAGTTCCGTTCACTCTTG 
               
               
                   
                   
                 CTATGGTGCGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 286 
                 Encoding Probe 276 
                 AGAGTGAGTAGTAGTGGAGTGGATATTCAGACA 
               
               
                   
                   
                 AGGTTTCACGTCGGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 287 
                 Encoding Probe 277 
                 AGAGTGAGTAGTAGTGGAGTAGAGTATTAACTA 
               
               
                   
                   
                 AAGTAGCCTCCAGGTGTGGAGGGATTGAAGGAT 
               
               
                   
                   
                 A 
               
               
                   
               
               
                 288 
                 Encoding Probe 278 
                 AGAGTGAGTAGTAGTGGAGTGTATCAGACAAGG 
               
               
                   
                   
                 TTTCACGTCGGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 289 
                 Encoding Probe 279 
                 AGAGTGAGTAGTAGTGGAGTTGATCATCATTATG 
               
               
                   
                   
                 TGTGCCCAAATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 290 
                 Encoding Probe 280 
                 AGAGTGAGTAGTAGTGGAGTAGATAAAACACAC 
               
               
                   
                   
                 ATAACTTAATGGGAACTGTGGAGGGATTGAAGG 
               
               
                   
                   
                 ATA 
               
               
                   
               
               
                 291 
                 Encoding Probe 281 
                 AGAGTGAGTAGTAGTGGAGTGAAGCTCATCTATT 
               
               
                   
                   
                 AGCGCAACCATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 292 
                 Encoding Probe 282 
                 AGAGTGAGTAGTAGTGGAGTATAATTCATGTTGC 
               
               
                   
                   
                 AATACCTACGAATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 293 
                 Encoding Probe 283 
                 AGAGTGAGTAGTAGTGGAGTCAGCCGCTAGGTCC 
               
               
                   
                   
                 GGTAGCAACGATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 294 
                 Encoding Probe 284 
                 AGAGTGAGTAGTAGTGGAGTGTCGGTTCACTCTT 
               
               
                   
                   
                 GCTATGGAGCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 295 
                 Encoding Probe 285 
                 AGAGTGAGTAGTAGTGGAGTAAAGATTAGCATC 
               
               
                   
                   
                 ACATCGCTCACTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 296 
                 Encoding Probe 286 
                 AGAGTGAGTAGTAGTGGAGTTAAGACTCGATTTC 
               
               
                   
                   
                 TCTACGGGAGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 297 
                 Encoding Probe 287 
                 AGAGTGAGTAGTAGTGGAGTGGCCTCTTTGCAGT 
               
               
                   
                   
                 TAGGCTAGGATTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 298 
                 Encoding Probe 288 
                 AGAGTGAGTAGTAGTGGAGTTCTTCAGCATAGAG 
               
               
                   
                   
                 TACCCCGCTATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 299 
                 Encoding Probe 289 
                 AGAGTGAGTAGTAGTGGAGTAGGTCGTCTGGTTT 
               
               
                   
                   
                 AGTTAGCGATATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 300 
                 Encoding Probe 290 
                 AGAGTGAGTAGTAGTGGAGTGGAATCACTATATA 
               
               
                   
                   
                 CTCTAGTACAGGTTAATAGGAAATGGTGGTAGTG 
               
               
                   
                   
                 T 
               
               
                   
               
               
                 301 
                 Encoding Probe 291 
                 AGAGTGAGTAGTAGTGGAGTCCGCCCGTTATCAT 
               
               
                   
                   
                 AGGCTCCATGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 302 
                 Encoding Probe 292 
                 AGAGTGAGTAGTAGTGGAGTAGGACTGAGATTG 
               
               
                   
                   
                 GCTTTAAGACTAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 303 
                 Encoding Probe 293 
                 AGAGTGAGTAGTAGTGGAGTAAAGGTCTACAAC 
               
               
                   
                   
                 ATGATACTATGCGCATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 304 
                 Encoding Probe 294 
                 AGAGTGAGTAGTAGTGGAGTAGGCCATGACACTT 
               
               
                   
                   
                 TTGTGTCTAGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 305 
                 Encoding Probe 295 
                 AGAGTGAGTAGTAGTGGAGTTACACTTTTGTGTC 
               
               
                   
                   
                 ATCCACACGAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 306 
                 Encoding Probe 296 
                 AGAGTGAGTAGTAGTGGAGTTGCCTCTTTGAATG 
               
               
                   
                   
                 AATAGCTGCAAGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 307 
                 Encoding Probe 297 
                 AGAGTGAGTAGTAGTGGAGTACGCGAAGAGAAA 
               
               
                   
                   
                 GCCTATCTCATCATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 308 
                 Encoding Probe 298 
                 AGAGTGAGTAGTAGTGGAGTTTATCTGGTTTAGT 
               
               
                   
                   
                 TAGCCTACACGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 309 
                 Encoding Probe 299 
                 AGAGTGAGTAGTAGTGGAGTCCTTTATCTGAGAT 
               
               
                   
                   
                 TGGTAATCCGCCTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 310 
                 Encoding Probe 300 
                 AGAGTGAGTAGTAGTGGAGTGATTCCAAGAGAC 
               
               
                   
                   
                 TTAACATCGACCGAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 311 
                 Encoding Probe 301 
                 AGAGTGAGTAGTAGTGGAGTGGTAGTCATCCAA 
               
               
                   
                   
                 GCACTTTTGTTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 312 
                 Encoding Probe 302 
                 AGAGTGAGTAGTAGTGGAGTGGAAAGTCATCCA 
               
               
                   
                   
                 AGCACTTTAGTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 313 
                 Encoding Probe 303 
                 AGAGTGAGTAGTAGTGGAGTAAAAAAGCGTACA 
               
               
                   
                   
                 ATGGTTAAGGGTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 314 
                 Encoding Probe 304 
                 AGAGTGAGTAGTAGTGGAGTAAGGCGTTCTAGG 
               
               
                   
                   
                 GCTTAACTAGAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 315 
                 Encoding Probe 305 
                 AGAGTGAGTAGTAGTGGAGTTAACGGGCTCGAA 
               
               
                   
                   
                 CTTGTTGTTCCATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 316 
                 Encoding Probe 306 
                 AGAGTGAGTAGTAGTGGAGTAATGTCACTTGGTA 
               
               
                   
                   
                 GATTTTCCAGAGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 317 
                 Encoding Probe 307 
                 AGAGTGAGTAGTAGTGGAGTGGTCCTACCAACGT 
               
               
                   
                   
                 TCTTCTCATTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 318 
                 Encoding Probe 308 
                 AGAGTGAGTAGTAGTGGAGTCTATGCTAAGGTTA 
               
               
                   
                   
                 ATCTATCATTTTTTTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 319 
                 Encoding Probe 309 
                 AGAGTGAGTAGTAGTGGAGTGGACCAGGTAATT 
               
               
                   
                   
                 CTTCTATAATGATATTATAGGAAATGGTGGTAGT 
               
               
                   
                   
                 GT 
               
               
                   
               
               
                 320 
                 Encoding Probe 310 
                 AGAGTGAGTAGTAGTGGAGTGTTCCGAAGTGTAA 
               
               
                   
                   
                 ACACTTCCCAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 321 
                 Encoding Probe 311 
                 AGAGTGAGTAGTAGTGGAGTGTTCATCAGTCTAG 
               
               
                   
                   
                 TGTAAACACGTTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 322 
                 Encoding Probe 312 
                 AGAGTGAGTAGTAGTGGAGTCTAGGATACTAGTC 
               
               
                   
                   
                 ATTAACTAGTGCCAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 323 
                 Encoding Probe 313 
                 AGAGTGAGTAGTAGTGGAGTCGTTCATCAGTCTA 
               
               
                   
                   
                 GTGTAAACACGTTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 324 
                 Encoding Probe 314 
                 TGTGATGGAAGTTAGAGGGTGTACTTGGACATGC 
               
               
                   
                   
                 ACTTCCAATGCGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 325 
                 Encoding Probe 315 
                 TGTGATGGAAGTTAGAGGGTAGTCTTATGCCATG 
               
               
                   
                   
                 CGGCATATTGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 326 
                 Encoding Probe 316 
                 TGTGATGGAAGTTAGAGGGTAGGCCACTACACCT 
               
               
                   
                   
                 AATGGTGATCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 327 
                 Encoding Probe 317 
                 TGTGATGGAAGTTAGAGGGTGATCCTAATGGTGT 
               
               
                   
                   
                 AGTCCACTCGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 328 
                 Encoding Probe 318 
                 TGTGATGGAAGTTAGAGGGTGATGTTCCGGTCTC 
               
               
                   
                   
                 ATCGGCTGGATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 329 
                 Encoding Probe 319 
                 TGTGATGGAAGTTAGAGGGTGGACACTCTTATGC 
               
               
                   
                   
                 CATGCGGCTATTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 330 
                 Encoding Probe 320 
                 TGTGATGGAAGTTAGAGGGTTCATTAATGCGTTT 
               
               
                   
                   
                 GCTGCAGGTGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 331 
                 Encoding Probe 321 
                 TGTGATGGAAGTTAGAGGGTCCCTTTCACCCTCT 
               
               
                   
                   
                 TTAGCGGTTATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 332 
                 Encoding Probe 322 
                 TGTGATGGAAGTTAGAGGGTATGCTACATACTTA 
               
               
                   
                   
                 TTCGCCCTTAATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 333 
                 Encoding Probe 323 
                 TGTGATGGAAGTTAGAGGGTGTGCATCACTCATT 
               
               
                   
                   
                 AACGAGCAAATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 334 
                 Encoding Probe 324 
                 TGTGATGGAAGTTAGAGGGTCAAGGGACGTTCA 
               
               
                   
                   
                 GTTACTAAACATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 335 
                 Encoding Probe 325 
                 TGTGATGGAAGTTAGAGGGTTCCACGTTCAGTTA 
               
               
                   
                   
                 CTAACGTGGATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 336 
                 Encoding Probe 326 
                 TGTGATGGAAGTTAGAGGGTTAGCCTAGGTGTTG 
               
               
                   
                   
                 TCAGCATAAGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 337 
                 Encoding Probe 327 
                 TGTGATGGAAGTTAGAGGGTTAGTCAACTATACT 
               
               
                   
                   
                 AACAGACTACCTATTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 338 
                 Encoding Probe 328 
                 TGTGATGGAAGTTAGAGGGTTCCACGTTCAGTTA 
               
               
                   
                   
                 CTAACGTCGAATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 339 
                 Encoding Probe 329 
                 TGTGATGGAAGTTAGAGGGTGGTCGGCATAAACT 
               
               
                   
                   
                 GTTATGCCCATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 340 
                 Encoding Probe 330 
                 TGTGATGGAAGTTAGAGGGTCGAGTATTCACTGA 
               
               
                   
                   
                 AAAGTAATATCCATATGTGGAGGGATTGAAGGAT 
               
               
                   
                   
                 A 
               
               
                   
               
               
                 341 
                 Encoding Probe 331 
                 TGTGATGGAAGTTAGAGGGTGAGCTTTCCAATTG 
               
               
                   
                   
                 AGTGCAACGTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 342 
                 Encoding Probe 332 
                 TGTGATGGAAGTTAGAGGGTCATGCATTTAACTC 
               
               
                   
                   
                 TACTCAAGACTGTATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 343 
                 Encoding Probe 333 
                 TGTGATGGAAGTTAGAGGGTCTTTCGCTACTATT 
               
               
                   
                   
                 ATTTCGCTAGGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 344 
                 Encoding Probe 334 
                 TGTGATGGAAGTTAGAGGGTCCAGGGCAGTTGTT 
               
               
                   
                   
                 TTCTCACATCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 345 
                 Encoding Probe 335 
                 TGTGATGGAAGTTAGAGGGTGACGCTGACCGAA 
               
               
                   
                   
                 GTCAGCACAGGTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 346 
                 Encoding Probe 336 
                 TGTGATGGAAGTTAGAGGGTGATACTAGCCTTCC 
               
               
                   
                   
                 ACTTCCAAGGATGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 347 
                 Encoding Probe 337 
                 TGTGATGGAAGTTAGAGGGTACCCTTCAATTCTG 
               
               
                   
                   
                 AGCTTCGGGCTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 348 
                 Encoding Probe 338 
                 TGTGATGGAAGTTAGAGGGTCAGCTCCAACTATC 
               
               
                   
                   
                 ACTAGCCTTGGTTGTGGAGGGATTGAAGGATA 
               
               
                   
               
               
                 349 
                 Encoding Probe 339 
                 TGTGATGGAAGTTAGAGGGTCCTCAGTTAATGAT 
               
               
                   
                   
                 AGTGTGTCGATTGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 350 
                 Encoding Probe 340 
                 TGTGATGGAAGTTAGAGGGTGGAGCCTTGGTTTT 
               
               
                   
                   
                 CCGGATTACGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 351 
                 Encoding Probe 341 
                 TGTGATGGAAGTTAGAGGGTGTGTCTCATCTCTG 
               
               
                   
                   
                 AAAACTTCCCACATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 352 
                 Encoding Probe 342 
                 TGTGATGGAAGTTAGAGGGTGTCACCCCATTAAG 
               
               
                   
                   
                 AGGCTCCGTGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 353 
                 Encoding Probe 343 
                 TGTGATGGAAGTTAGAGGGTCCACGTCAATGAGC 
               
               
                   
                   
                 AAAGGTAAATATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 354 
                 Encoding Probe 344 
                 TGTGATGGAAGTTAGAGGGTGTAAGCTCACAATA 
               
               
                   
                   
                 TGTGCATAAAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 355 
                 Encoding Probe 345 
                 TGTGATGGAAGTTAGAGGGTGATACACACACTGA 
               
               
                   
                   
                 TTCAGGCAGAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 356 
                 Encoding Probe 346 
                 TGTGATGGAAGTTAGAGGGTAGTCTTGGTTTTCC 
               
               
                   
                   
                 GGATTTGGGAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 357 
                 Encoding Probe 347 
                 TGTGATGGAAGTTAGAGGGTACCTCAGTTAATGA 
               
               
                   
                   
                 TAGTGTGTCGTTTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 358 
                 Encoding Probe 348 
                 TGTGATGGAAGTTAGAGGGTGAGCCTTGGTTTTC 
               
               
                   
                   
                 CGGATTTCGGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 359 
                 Encoding Probe 349 
                 TGTGATGGAAGTTAGAGGGTGTATCATCTCTGAA 
               
               
                   
                   
                 AACTTCCGACCATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 360 
                 Encoding Probe 350 
                 TGTGATGGAAGTTAGAGGGTGTGCTCAGCCTTGG 
               
               
                   
                   
                 TTTTCCGCTAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 361 
                 Encoding Probe 351 
                 TGTGATGGAAGTTAGAGGGTTGCGTCACCCCATT 
               
               
                   
                   
                 AAGAGGCAGGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 362 
                 Encoding Probe 352 
                 TGTGATGGAAGTTAGAGGGTCATGTCAATGAGCA 
               
               
                   
                   
                 AAGGTATTAAGAAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 363 
                 Encoding Probe 353 
                 TGTGATGGAAGTTAGAGGGTGTAAGCTCACAATA 
               
               
                   
                   
                 TGTGCATTAAAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 364 
                 Encoding Probe 354 
                 TGTGATGGAAGTTAGAGGGTGAAACTAACACAC 
               
               
                   
                   
                 ACACTGATTGTCATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 365 
                 Encoding Probe 355 
                 TGTGATGGAAGTTAGAGGGTCTAAGTTAATGATA 
               
               
                   
                   
                 GTGTGTCGATTGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 366 
                 Encoding Probe 356 
                 TGTGATGGAAGTTAGAGGGTGTGTCTCATCTCTG 
               
               
                   
                   
                 AAAACTTCCGACCATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 367 
                 Encoding Probe 357 
                 TGTGATGGAAGTTAGAGGGTAGGAAGGCACATT 
               
               
                   
                   
                 CTCATCTCACTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 368 
                 Encoding Probe 358 
                 TGTGATGGAAGTTAGAGGGTCGTCACCCCATTAA 
               
               
                   
                   
                 GAGGCTCGGTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 369 
                 Encoding Probe 359 
                 TGTGATGGAAGTTAGAGGGTGCGTCACCCCATTA 
               
               
                   
                   
                 AGAGGCTAGGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 370 
                 Encoding Probe 360 
                 TGTGATGGAAGTTAGAGGGTCATGTCAATGAGCA 
               
               
                   
                   
                 AAGGTATTATGAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 371 
                 Encoding Probe 361 
                 TGTGATGGAAGTTAGAGGGTTAGGCTCACAATAT 
               
               
                   
                   
                 GTGCATTAAAATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 372 
                 Encoding Probe 362 
                 TGTGATGGAAGTTAGAGGGTTGACACACACACTG 
               
               
                   
                   
                 ATTCAGGGAGATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 373 
                 Encoding Probe 363 
                 TGTGATGGAAGTTAGAGGGTCCTCAGTTAATGAT 
               
               
                   
                   
                 AGTGTGTCGTTTATAGGAAATGGTGGTAGTGT 
               
               
                   
               
               
                 374 
                 Encoding Probe 364 
                 TGTGATGGAAGTTAGAGGGTGTTGTGAACAAACT 
               
               
                   
                   
                 TTCGACTACTCCAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 375 
                 Encoding Probe 365 
                 TGTGATGGAAGTTAGAGGGTGGACGCTTAAAAC 
               
               
                   
                   
                 GAATAATGGTGGATGAGAGTGAGTAGTAGTGGA 
               
               
                   
                   
                 GT 
               
               
                   
               
               
                 376 
                 Encoding Probe 366 
                 TGTGATGGAAGTTAGAGGGTGTACTTAAAACGAA 
               
               
                   
                   
                 TAATGGTGGTAGTCAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 377 
                 Encoding Probe 367 
                 TGTGATGGAAGTTAGAGGGTGGTGTCCTTACGGA 
               
               
                   
                   
                 CAATCCAGTCAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 378 
                 Encoding Probe 368 
                 TGTGATGGAAGTTAGAGGGTCAGACTCTTGCGGA 
               
               
                   
                   
                 ACGTAAGAGGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 379 
                 Encoding Probe 369 
                 TGTGATGGAAGTTAGAGGGTTTGCTCGAGGAAAC 
               
               
                   
                   
                 AATTTCCAGAAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 380 
                 Encoding Probe 370 
                 TGTGATGGAAGTTAGAGGGTCGACTCCATAAATG 
               
               
                   
                   
                 GTTACTCCACGCCAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 381 
                 Encoding Probe 371 
                 TGTGATGGAAGTTAGAGGGTTAACCTAACACTCA 
               
               
                   
                   
                 ATCTCACTGCTTCCTAGAGTGAGTAGTAGTGGAG 
               
               
                   
                   
                 T 
               
               
                   
               
               
                 382 
                 Encoding Probe 372 
                 TGTGATGGAAGTTAGAGGGTTTAGGTAACCCGAT 
               
               
                   
                   
                 AAGGGCCGGAAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 383 
                 Encoding Probe 373 
                 TGTGATGGAAGTTAGAGGGTCTCAGCTCCTTATC 
               
               
                   
                   
                 TGTTCGCTGCTAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 384 
                 Encoding Probe 374 
                 TGTGATGGAAGTTAGAGGGTCACCTCCTTGCCAT 
               
               
                   
                   
                 TGTCACCAATAAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 385 
                 Encoding Probe 375 
                 TGTGATGGAAGTTAGAGGGTGCTCGAGGAAACA 
               
               
                   
                   
                 ATTTCCTCAGGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 386 
                 Encoding Probe 376 
                 TGTGATGGAAGTTAGAGGGTAATTACACGTTTGT 
               
               
                   
                   
                 TCTTCCCATTAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 387 
                 Encoding Probe 377 
                 TGTGATGGAAGTTAGAGGGTGTGAAGAGTGAAC 
               
               
                   
                   
                 AAACTTTCGTGAAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 388 
                 Encoding Probe 378 
                 TGTGATGGAAGTTAGAGGGTTACTCGTCTAGTCT 
               
               
                   
                   
                 GTTCTTTTGTAAGAGAGAGTGAGTAGTAGTGGAG 
               
               
                   
                   
                 T 
               
               
                   
               
               
                 389 
                 Encoding Probe 379 
                 TGTGATGGAAGTTAGAGGGTAGGCGCTAACGTCA 
               
               
                   
                   
                 AAGGAGCTTCAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 390 
                 Encoding Probe 380 
                 TGTGATGGAAGTTAGAGGGTGATAGTGATAGCA 
               
               
                   
                   
                 AAACCATCTTTCTGAAGAGTGAGTAGTAGTGGAG 
               
               
                   
                   
                 T 
               
               
                   
               
               
                 391 
                 Encoding Probe 381 
                 TGTGATGGAAGTTAGAGGGTTCGGCTCCTTATCT 
               
               
                   
                   
                 GTTCGCTCCTGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 392 
                 Encoding Probe 382 
                 TGTGATGGAAGTTAGAGGGTCTCAGCTCCTTATC 
               
               
                   
                   
                 TGTTCGCAGCAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 393 
                 Encoding Probe 383 
                 TGTGATGGAAGTTAGAGGGTCACCTCCTTGCCAT 
               
               
                   
                   
                 TGTCACCTTAAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 394 
                 Encoding Probe 384 
                 TGTGATGGAAGTTAGAGGGTGGACACTCAATCTC 
               
               
                   
                   
                 ACTGCTTCCTAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 395 
                 Encoding Probe 385 
                 TGTGATGGAAGTTAGAGGGTGCCACCACAATTCT 
               
               
                   
                   
                 AGCTAGAGCGAAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 396 
                 Encoding Probe 386 
                 TGTGATGGAAGTTAGAGGGTCGGACACTCAATCT 
               
               
                   
                   
                 CACTGCTACCAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 397 
                 Encoding Probe 387 
                 TGTGATGGAAGTTAGAGGGTATCCTCACGTATCT 
               
               
                   
                   
                 CAGGCTCCATGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
               
                 398 
                 Encoding Probe 388 
                 TGTGATGGAAGTTAGAGGGTAAGCAGCTGCACAT 
               
               
                   
                   
                 ATCGCTAACGAGAGTGAGTAGTAGTGGAGT 
               
               
                   
               
            
           
         
       
     
     Example 2.3 
       FIG.  5    shows the ability of the HiPR-FISH to differentiate drug-resistant from drug-susceptible microbes in a sample. The following methodology was employed. Carbapenem-resistant or -susceptible  Pseudomonas aeruginosa  were cultured in liquid tryptic soy broth for several passages. An 8-well device was constructed where each well was filled with 25 μL of tryptic soy agar at 42° C. with various concentrations of meropenem and allowed to dry to create a growth pad for bacteria. For both resistant and susceptible cultures, 1 μL of culture suspension was deposited at the center of each pad and the liquid was allowed to dry/absorb. A #1 coverslip was used to seal the bottom of the device. The device was placed on a custom built stage that enabled temperature regulation on a Nikon TiE widefield epifluorescence microscope. The custom stage contained a chamber that used two Peltier units to keep the bottom of the stage at 37° C. for incubation and the top at 42° C. to prevent condensation. A 40×phase contrast objective was used to continuously image a single field of view in each well of the device every 30 seconds for two hours. At the conclusion of the experiment, 2% formaldehyde was added to each well to fix colonies for down stream assays. 
     Example 3. Identification of Fungi 
       FIG.  6    shows the identification of different fungi species including  C. tropicalis, C. glabrata , and  C. albicans , using the following methodology. 
     Suspensions of individual monocultures were fixed by adding an equal volume of 2% formaldehyde, mixing, and incubating for 90 minutes at room temperature. Fixed cultures were then washed with 1×PBS and resuspended in 50% ethanol. Suspensions were deposited onto glass microscope slides until 50% ethanol had evaporated. Zymolysae (5 U per mL in a buffer with 1.2 M sorbitol and 0.1 M potassium phosphate buffer, pH 7.5) was deposited onto each dry specimen to permeabilize the outer membrane and incubated for 90 minutes at 30° C., the slides were then washed with 1×PBS. An encoding probe hybridization buffer (2×SSC, 10% dextran sulfate, 10% ethylene carbonate, 5×Denhardt&#39;s solution, 0.01% SDS) with probes designed for the fungal species (at roughly 200 nM) was deposited on cells and incubated for two hours at 37° C. A wash buffer (5 mM EDTA, 20 mM Tris HCl, 215 mM NaCl) was then deposited on specimens for fifteen minutes at 37° C. to remove unbound probes. A buffer containing readout probes (10 readout probes, each at 400 nM; buffer made up of 2×SSC, 10% dextran sulfate, 10% ethylene carbonate, 5×Denhardt&#39;s solution, 0.01% SDS) was incubated for one hour at room temperature. A second round of wash buffer was deposited on specimens for fifteen minutes at 37° C. to remove unbound probes. The specimens were mounted with Prolong Glass and a coverslip was placed directly over the specimens for imaging on a confocal microscope. 
     Table 3 shows the sequences of the encoding probes used in this example. The readout probes are shown in Table 1. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Encoding probes used in Example 3 
               
            
           
           
               
               
               
            
               
                 SEQ ID 
                   
                   
               
               
                 NO: 
                 Probe Name 
                 Sequence 
               
               
                   
               
               
                 399 
                 Encoding Probe 389 
                 AGGGTGTGTTTGTAAAGGGTACTTCCCCGTGGTTG 
               
               
                   
                   
                 AGTCAAAAAT 
               
               
                   
               
               
                 400 
                 Encoding Probe 390 
                 TGTGGAGGGATTGAAGGATAACTTCCCCGTGGTT 
               
               
                   
                   
                 GAGTCAAAAAT 
               
               
                   
               
               
                 401 
                 Encoding Probe 391 
                 AGGGTGTGTTTGTAAAGGGTACCCCAGACTTGGC 
               
               
                   
                   
                 CTTCCAATTGTAGG 
               
               
                   
               
               
                 402 
                 Encoding Probe 392 
                 TGTGGAGGGATTGAAGGATAACCCCAGACTTGGC 
               
               
                   
                   
                 CTTCCAATTGTAGG 
               
               
                   
               
               
                 403 
                 Encoding Probe 393 
                 AGGGTGTGTTTGTAAAGGGTGAGTTCCAGAATGA 
               
               
                   
                   
                 GGTTGCCAGG 
               
               
                   
               
               
                 404 
                 Encoding Probe 394 
                 TGTGGAGGGATTGAAGGATAGAGTTCCAGAATGA 
               
               
                   
                   
                 GGTTGCCAGG 
               
               
                   
               
               
                 405 
                 Encoding Probe 395 
                 AGGGTGTGTTTGTAAAGGGTAGAGTTCCAGAATG 
               
               
                   
                   
                 AGGTTGCCAGG 
               
               
                   
               
               
                 406 
                 Encoding Probe 396 
                 TGTGGAGGGATTGAAGGATAAGAGTTCCAGAATG 
               
               
                   
                   
                 AGGTTGCCAGG 
               
               
                   
               
               
                 407 
                 Encoding Probe 397 
                 AGGGTGTGTTTGTAAAGGGTAGGGTTCGCCATAA 
               
               
                   
                   
                 ATGGCTACCGTC 
               
               
                   
               
               
                 408 
                 Encoding Probe 398 
                 TGTGGAGGGATTGAAGGATAAGGGTTCGCCATAA 
               
               
                   
                   
                 ATGGCTACCGTC 
               
               
                   
               
               
                 409 
                 Encoding Probe 399 
                 AGGGTGTGTTTGTAAAGGGTTGACATCGACTTGG 
               
               
                   
                   
                 AGTCGATTCA 
               
               
                   
               
               
                 410 
                 Encoding Probe 400 
                 TGTGGAGGGATTGAAGGATATGACATCGACTTGG 
               
               
                   
                   
                 AGTCGATTCA 
               
               
                   
               
               
                 411 
                 Encoding Probe 401 
                 AGGGTGTGTTTGTAAAGGGTCGTTGACTACTGGC 
               
               
                   
                   
                 AGGATCAACCACTA 
               
               
                   
               
               
                 412 
                 Encoding Probe 402 
                 TGTGGAGGGATTGAAGGATACGTTGACTACTGGC 
               
               
                   
                   
                 AGGATCAACCACTA 
               
               
                   
               
               
                 413 
                 Encoding Probe 403 
                 AGGGTGTGTTTGTAAAGGGTACTTCCCCGTGGTTG 
               
               
                   
                   
                 AGTCAATAA 
               
               
                   
               
               
                 414 
                 Encoding Probe 404 
                 TGTGGAGGGATTGAAGGATAACTTCCCCGTGGTT 
               
               
                   
                   
                 GAGTCAATAA 
               
               
                   
               
               
                 415 
                 Encoding Probe 405 
                 AGGGTGTGTTTGTAAAGGGTGGATTCGCCATAAA 
               
               
                   
                   
                 TGGCTACCGTC 
               
               
                   
               
               
                 416 
                 Encoding Probe 406 
                 TGTGGAGGGATTGAAGGATAGGATTCGCCATAAA 
               
               
                   
                   
                 TGGCTACCGTC 
               
               
                   
               
               
                 417 
                 Encoding Probe 407 
                 AGGGTGTGTTTGTAAAGGGTGTAACTTGGAGTCG 
               
               
                   
                   
                 ATAGTCCCAGA 
               
               
                   
               
               
                 418 
                 Encoding Probe 408 
                 TGTGGAGGGATTGAAGGATAGTAACTTGGAGTCG 
               
               
                   
                   
                 ATAGTCCCAGA 
               
               
                   
               
               
                 419 
                 Encoding Probe 409 
                 AGGGTGTGTTTGTAAAGGGTTCGATGACTACTGG 
               
               
                   
                   
                 CAGGATCAACCACTA 
               
               
                   
               
               
                 420 
                 Encoding Probe 410 
                 TGTGGAGGGATTGAAGGATATCGATGACTACTGG 
               
               
                   
                   
                 CAGGATCAACCACTA 
               
               
                   
               
               
                 421 
                 Encoding Probe 411 
                 AGGGTGTGTTTGTAAAGGGTAGTACCTCCCCTGA 
               
               
                   
                   
                 ATCGGGATTCCC 
               
               
                   
               
               
                 422 
                 Encoding Probe 412 
                 TGTGGAGGGATTGAAGGATAAGTACCTCCCCTGA 
               
               
                   
                   
                 ATCGGGATTCCC 
               
               
                   
               
               
                 423 
                 Encoding Probe 413 
                 AGAGTGAGTAGTAGTGGAGTAACTTGCTTTTCTTC 
               
               
                   
                   
                 CTCTAATGACCTTC 
               
               
                   
               
               
                 424 
                 Encoding Probe 414 
                 TTGGAGGTGTAGGGAGTAAAAACTTGCTTTTCTTC 
               
               
                   
                   
                 CTCTAATGACCTTC 
               
               
                   
               
               
                 425 
                 Encoding Probe 415 
                 AGAGTGAGTAGTAGTGGAGTACGTGCTTTTCTTCC 
               
               
                   
                   
                 TCTAATGACCATCA 
               
               
                   
               
               
                 426 
                 Encoding Probe 416 
                 TTGGAGGTGTAGGGAGTAAAACGTGCTTTTCTTCC 
               
               
                   
                   
                 TCTAATGACCATCA 
               
               
                   
               
               
                 427 
                 Encoding Probe 417 
                 AGAGTGAGTAGTAGTGGAGTCGAGCTTTTCTTCCT 
               
               
                   
                   
                 CTAATGACCAACAA 
               
               
                   
               
               
                 428 
                 Encoding Probe 418 
                 TTGGAGGTGTAGGGAGTAAACGAGCTTTTCTTCCT 
               
               
                   
                   
                 CTAATGACCAACAA 
               
               
                   
               
               
                 429 
                 Encoding Probe 419 
                 AGAGTGAGTAGTAGTGGAGTTGTCATGGCTAATC 
               
               
                   
                   
                 TAGCGGGTTA 
               
               
                   
               
               
                 430 
                 Encoding Probe 420 
                 TTGGAGGTGTAGGGAGTAAATGTCATGGCTAATC 
               
               
                   
                   
                 TAGCGGGTTA 
               
               
                   
               
               
                 431 
                 Encoding Probe 421 
                 AGAGTGAGTAGTAGTGGAGTCTGGCATGGCTAAT 
               
               
                   
                   
                 CTAGCGGCTA 
               
               
                   
               
               
                 432 
                 Encoding Probe 422 
                 TTGGAGGTGTAGGGAGTAAACTGGCATGGCTAAT 
               
               
                   
                   
                 CTAGCGGCTA 
               
               
                   
               
               
                 433 
                 Encoding Probe 423 
                 AGAGTGAGTAGTAGTGGAGTGGATTCGCCAAAAG 
               
               
                   
                   
                 GCTAGCCAGTTC 
               
               
                   
               
               
                 434 
                 Encoding Probe 424 
                 TTGGAGGTGTAGGGAGTAAAGGATTCGCCAAAAG 
               
               
                   
                   
                 GCTAGCCAGTTC 
               
               
                   
               
               
                 435 
                 Encoding Probe 425 
                 AGAGTGAGTAGTAGTGGAGTCTGGCATGGCTAAT 
               
               
                   
                   
                 CTAGCGGGAATA 
               
               
                   
               
               
                 436 
                 Encoding Probe 426 
                 TTGGAGGTGTAGGGAGTAAACTGGCATGGCTAAT 
               
               
                   
                   
                 CTAGCGGGAATA 
               
               
                   
               
               
                 437 
                 Encoding Probe 427 
                 AGAGTGAGTAGTAGTGGAGTACCCGCCAAAAGGC 
               
               
                   
                   
                 TAGCCAGAACCT 
               
               
                   
               
               
                 438 
                 Encoding Probe 428 
                 TTGGAGGTGTAGGGAGTAAAACCCGCCAAAAGGC 
               
               
                   
                   
                 TAGCCAGAACCT 
               
               
                   
               
               
                 439 
                 Encoding Probe 429 
                 AGAGTGAGTAGTAGTGGAGTTCTTGCATGGCTAA 
               
               
                   
                   
                 TCTAGCGGGAGTT 
               
               
                   
               
               
                 440 
                 Encoding Probe 430 
                 TTGGAGGTGTAGGGAGTAAATCTTGCATGGCTAA 
               
               
                   
                   
                 TCTAGCGGGAGTT 
               
               
                   
               
               
                 441 
                 Encoding Probe 431 
                 AGAGTGAGTAGTAGTGGAGTCTGGCATGGCTAAT 
               
               
                   
                   
                 CTAGCGGGTTA 
               
               
                   
               
               
                 442 
                 Encoding Probe 432 
                 TTGGAGGTGTAGGGAGTAAACTGGCATGGCTAAT 
               
               
                   
                   
                 CTAGCGGGTTA 
               
               
                   
               
               
                 443 
                 Encoding Probe 433 
                 AGAGTGAGTAGTAGTGGAGTTGTCATGGCTAATC 
               
               
                   
                   
                 TAGCGGGAATA 
               
               
                   
               
               
                 444 
                 Encoding Probe 434 
                 TTGGAGGTGTAGGGAGTAAATGTCATGGCTAATC 
               
               
                   
                   
                 TAGCGGGAATA 
               
               
                   
               
               
                 445 
                 Encoding Probe 435 
                 AGAGTGAGTAGTAGTGGAGTAGGGTTCGCCAAAA 
               
               
                   
                   
                 GGCTAGCGTC 
               
               
                   
               
               
                 446 
                 Encoding Probe 436 
                 TTGGAGGTGTAGGGAGTAAAAGGGTTCGCCAAAA 
               
               
                   
                   
                 GGCTAGCGTC 
               
               
                   
               
               
                 447 
                 Encoding Probe 437 
                 TGTGGAGGGATTGAAGGATATATTCTCTTCCAAG 
               
               
                   
                   
                 AGGTCGAGATTTATT 
               
               
                   
               
               
                 448 
                 Encoding Probe 438 
                 TTGGAGGTGTAGGGAGTAAATATTCTCTTCCAAG 
               
               
                   
                   
                 AGGTCGAGATTTATT 
               
               
                   
               
               
                 449 
                 Encoding Probe 439 
                 TGTGGAGGGATTGAAGGATAGAGATTACCGCGGG 
               
               
                   
                   
                 CTGCTGGGTG 
               
               
                   
               
               
                 450 
                 Encoding Probe 440 
                 TTGGAGGTGTAGGGAGTAAAGAGATTACCGCGGG 
               
               
                   
                   
                 CTGCTGGGTG 
               
               
                   
               
               
                 451 
                 Encoding Probe 441 
                 TGTGGAGGGATTGAAGGATAGTCTCTCCGCTCTG 
               
               
                   
                   
                 AAGTGGAGTCCGG 
               
               
                   
               
               
                 452 
                 Encoding Probe 442 
                 TTGGAGGTGTAGGGAGTAAAGTCTCTCCGCTCTG 
               
               
                   
                   
                 AAGTGGAGTCCGG 
               
               
                   
               
               
                 453 
                 Encoding Probe 443 
                 TGTGGAGGGATTGAAGGATAAAAGTACACGAAAA 
               
               
                   
                   
                 AATCGGACCGGAGT 
               
               
                   
               
               
                 454 
                 Encoding Probe 444 
                 TTGGAGGTGTAGGGAGTAAAAAAGTACACGAAAA 
               
               
                   
                   
                 AATCGGACCGGAGT 
               
               
                   
               
               
                 455 
                 Encoding Probe 445 
                 TGTGGAGGGATTGAAGGATAGTACAGTACACGAA 
               
               
                   
                   
                 AAAATCGGACCGCGG 
               
               
                   
               
               
                 456 
                 Encoding Probe 446 
                 TTGGAGGTGTAGGGAGTAAAGTACAGTACACGAA 
               
               
                   
                   
                 AAAATCGGACCGCGG 
               
               
                   
               
               
                 457 
                 Encoding Probe 447 
                 TGTGGAGGGATTGAAGGATAGTGCCTCCCTGTGT 
               
               
                   
                   
                 CAGGATTCCC 
               
               
                   
               
               
                 458 
                 Encoding Probe 448 
                 TTGGAGGTGTAGGGAGTAAAGTGCCTCCCTGTGT 
               
               
                   
                   
                 CAGGATTCCC 
               
               
                   
               
               
                 459 
                 Encoding Probe 449 
                 TGTGGAGGGATTGAAGGATAGTGCCTCCCTGTGT 
               
               
                   
                   
                 CAGGATTGCCA 
               
               
                   
               
               
                 460 
                 Encoding Probe 450 
                 TTGGAGGTGTAGGGAGTAAAGTGCCTCCCTGTGT 
               
               
                   
                   
                 CAGGATTGCCA 
               
               
                   
               
               
                 461 
                 Encoding Probe 451 
                 TGTGGAGGGATTGAAGGATACATGTGCCGAGTGG 
               
               
                   
                   
                 GTCACTAATTT 
               
               
                   
               
               
                 462 
                 Encoding Probe 452 
                 TTGGAGGTGTAGGGAGTAAACATGTGCCGAGTGG 
               
               
                   
                   
                 GTCACTAATTT 
               
               
                   
               
               
                 463 
                 Encoding Probe 453 
                 TGTGGAGGGATTGAAGGATACTCGGTCACTAAAA 
               
               
                   
                   
                 AAACACCACCCGTAG 
               
               
                   
               
               
                 464 
                 Encoding Probe 454 
                 TTGGAGGTGTAGGGAGTAAACTCGGTCACTAAAA 
               
               
                   
                   
                 AAACACCACCCGTAG 
               
               
                   
               
               
                 465 
                 Encoding Probe 455 
                 TGTGGAGGGATTGAAGGATAAGAGCCAAGGTTAG 
               
               
                   
                   
                 ACTCGCTGCGA 
               
               
                   
               
               
                 466 
                 Encoding Probe 456 
                 TTGGAGGTGTAGGGAGTAAAAGAGCCAAGGTTAG 
               
               
                   
                   
                 ACTCGCTGCGA 
               
               
                   
               
               
                 467 
                 Encoding Probe 457 
                 TGTGGAGGGATTGAAGGATACTGGCATGGCTTAA 
               
               
                   
                   
                 TTTTTAGACAAAATG 
               
               
                   
               
               
                 468 
                 Encoding Probe 458 
                 TTGGAGGTGTAGGGAGTAAACTGGCATGGCTTAA 
               
               
                   
                   
                 TTTTTAGACAAAATG 
               
               
                   
               
               
                 469 
                 Encoding Probe 459 
                 TGTGGAGGGATTGAAGGATATTAATCTCTTCCAA 
               
               
                   
                   
                 GAGGTCGAGATTAAT 
               
               
                   
               
               
                 470 
                 Encoding Probe 460 
                 TTGGAGGTGTAGGGAGTAAATTAATCTCTTCCAA 
               
               
                   
                   
                 GAGGTCGAGATTAAT 
               
               
                   
               
            
           
         
       
     
     Example 4. HiPR-FAST One Pot 
       Escherichia coli  ( E. coli ) cells were cultured at 30° C. for several passages prior to the start of the experiment. At experiment passage, cultured  E. coli  were grown in suspension at 30° C. ambient temperature for ninety minutes. Then, their vessel was sealed and placed in a water bath at 46° C. for five minutes. Immediately following the heat shock, the vessel was placed on ice for one minute before a volume of 2% formaldehyde (equal to the volume of the suspension) was added to the suspension and mixed for suspension. Fixing cells were incubated at room temperature for one hour. After one hour, fixed cells were washed with 1×PBS and resuspended in 50% ethanol. A small volume (0.75 μL) was deposited on a glass slide and allowed to dry. The deposition was then rehydrated with 10 mg/ml lysozyme and placed at 37° C. for 15 minutes to encourage permeabilization. Cells were then washed with 1×PBS for ten minutes at room temperature. A hybridization buffer (2×SSC, 10% dextran sulfate, 10% ethylene carbonate, 5×Denhardt&#39;s solution, 0.01% SDS) containing rRNA (1 μM per species) and mRNA (1 μM per gene) was added to cells and the slide was placed at 37° C. for one hour. Immediately following hybridization, cells were incubated in wash buffer (5 mM EDTA, 20 mM Tris HCl, 215 mM NaCl) for 15 minutes at 48° C. Finally, the wash buffer was removed and the cells were mounted with Prolong Glass under a #1 coverslip for imaging. 
       FIGS.  7 A- 7 C  shows gene expression measurement enable rapid detection of stress response in HiPR-FISH. Table 4 shows the sequences of the encoding probes used in this example. The readout probes are shown in Table 1. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Encoding Probes used in Example 4 
               
            
           
           
               
               
               
               
            
               
                 SEQ 
                   
                   
                   
               
               
                 ID 
                 Tar- 
                 Probe 
                   
               
               
                 NO: 
                 get 
                 Name 
                 Sequence (in 5′ to 3′ order) 
               
               
                   
               
               
                 471 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTCCTCAGTTAAT 
               
               
                   
                   
                 Probe 461 
                 GATAGTGTGTCGATTG 
               
               
                   
               
               
                 472 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTGGAGCCTTGG 
               
               
                   
                   
                 Probe 462 
                 TTTTCCGGATTACG 
               
               
                   
               
               
                 473 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTGTGTCTCATCT 
               
               
                   
                   
                 Probe 463 
                 CTGAAAACTTCCCAC 
               
               
                   
               
               
                 474 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTGTCACCCCATT 
               
               
                   
                   
                 Probe 464 
                 AAGAGGCTCCGTG 
               
               
                   
               
               
                 475 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTCCACGTCAATG 
               
               
                   
                   
                 Probe 465 
                 AGCAAAGGTAAAT 
               
               
                   
               
               
                 476 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTGTAAGCTCAC 
               
               
                   
                   
                 Probe 466 
                 AATATGTGCATAAA 
               
               
                   
               
               
                 477 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTGATACACACA 
               
               
                   
                   
                 Probe 467 
                 CTGATTCAGGCAGA 
               
               
                   
               
               
                 478 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTAGTCTTGGTTT 
               
               
                   
                   
                 Probe 468 
                 TCCGGATTTGGGA 
               
               
                   
               
               
                 479 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTACCTCAGTTAA 
               
               
                   
                   
                 Probe 469 
                 TGATAGTGTGTCGTTT 
               
               
                   
               
               
                 480 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTGAGCCTTGGTT 
               
               
                   
                   
                 Probe 470 
                 TTCCGGATTTCGG 
               
               
                   
               
               
                 481 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTGTATCATCTCT 
               
               
                   
                   
                 Probe 471 
                 GAAAACTTCCGACC 
               
               
                   
               
               
                 482 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTGTGCTCAGCCT 
               
               
                   
                   
                 Probe 472 
                 TGGTTTTCCGCTA 
               
               
                   
               
               
                 483 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTTGCGTCACCCC 
               
               
                   
                   
                 Probe 473 
                 ATTAAGAGGCAGG 
               
               
                   
               
               
                 484 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTCATGTCAATG 
               
               
                   
                   
                 Probe 474 
                 AGCAAAGGTATTAAGAA 
               
               
                   
               
               
                 485 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTGTAAGCTCAC 
               
               
                   
                   
                 Probe 475 
                 AATATGTGCATTAAA 
               
               
                   
               
               
                 486 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTGAAACTAACA 
               
               
                   
                   
                 Probe 476 
                 CACACACTGATTGTC 
               
               
                   
               
               
                 487 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTCTAAGTTAATG 
               
               
                   
                   
                 Probe 477 
                 ATAGTGTGTCGATTG 
               
               
                   
               
               
                 488 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTGTGTCTCATCT 
               
               
                   
                   
                 Probe 478 
                 CTGAAAACTTCCGACC 
               
               
                   
               
               
                 489 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTAGGAAGGCAC 
               
               
                   
                   
                 Probe 479 
                 ATTCTCATCTCACT 
               
               
                   
               
               
                 490 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTCGTCACCCCAT 
               
               
                   
                   
                 Probe 480 
                 TAAGAGGCTCGGT 
               
               
                   
               
               
                 491 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTGCGTCACCCCA 
               
               
                   
                   
                 Probe 481 
                 TTAAGAGGCTAGG 
               
               
                   
               
               
                 492 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTCATGTCAATG 
               
               
                   
                   
                 Probe 482 
                 AGCAAAGGTATTATGA 
               
               
                   
               
               
                 493 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTTAGGCTCACA 
               
               
                   
                   
                 Probe 483 
                 ATATGTGCATTAAA 
               
               
                   
               
               
                 494 
                 rRNA 
                 Encoding 
                 ATAGGAAATGGTGGTAGTGTTGACACACAC 
               
               
                   
                   
                 Probe 484 
                 ACTGATTCAGGGAG 
               
               
                   
               
               
                 495 
                 rRNA 
                 Encoding 
                 AGGGTGTGTTTGTAAAGGGTCCTCAGTTAAT 
               
               
                   
                   
                 Probe 485 
                 GATAGTGTGTCGTTT 
               
               
                   
               
               
                 496 
                 mRNA 
                 Encoding 
                 CGTCGGAGTGGGTTCAGTCTATCATCGCCAG 
               
               
                   
                   
                 Probe 486 
                 CGCCTTACAAAGCTCT 
               
               
                   
               
               
                 497 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTCGGTTCGAGC 
               
               
                   
                   
                 Probe 487 
                 TGCGTTGCGGCTTCCA 
               
               
                   
               
               
                 498 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTAAGACCACGC 
               
               
                   
                   
                 Probe 488 
                 GCCAGTGCAGGTTTCA 
               
               
                   
               
               
                 499 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTGCACGCTGCT 
               
               
                   
                   
                 Probe 489 
                 GCAACAATTGCCGGGT 
               
               
                   
               
               
                 500 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTGTCTACGCGG 
               
               
                   
                   
                 Probe 490 
                 CGGCCTTTCAACCCTT 
               
               
                   
               
               
                 501 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTTTAACGCTGC 
               
               
                   
                   
                 Probe 491 
                 GCCAGACCTTCAACGA 
               
               
                   
               
               
                 502 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTCAGAAGCGTC 
               
               
                   
                   
                 Probe 492 
                 GTGGCACCTACGCAGT 
               
               
                   
               
               
                 503 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTCAGACCCACA 
               
               
                   
                   
                 Probe 493 
                 CGGCGAGCCTGTTCAA 
               
               
                   
               
               
                 504 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTCGCACGACGC 
               
               
                   
                   
                 Probe 494 
                 ACCGCTTCGGTCAGAT 
               
               
                   
               
               
                 505 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTAAAATCCGGC 
               
               
                   
                   
                 Probe 495 
                 AGCTGACGGTCAGCAA 
               
               
                   
               
               
                 506 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTAAATCGCGCT 
               
               
                   
                   
                 Probe 496 
                 CGCTTTCCATCATGCG 
               
               
                   
               
               
                 507 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTGAATACCCGC 
               
               
                   
                   
                 Probe 497 
                 GCCGACCATGGTATGT 
               
               
                   
               
               
                 508 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTCTATGGGCAT 
               
               
                   
                   
                 Probe 498 
                 CGGCAAGAGCAAGCTG 
               
               
                   
               
               
                 509 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTACGTTTCAGG 
               
               
                   
                   
                 Probe 499 
                 ATGTCGGCCAGCGTGC 
               
               
                   
               
               
                 510 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTAGCATACGGA 
               
               
                   
                   
                 Probe 500 
                 CCATCGCCTCGTCGCT 
               
               
                   
               
               
                 511 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTGCATGCAGCA 
               
               
                   
                   
                 Probe 501 
                 CCTGAATGGTACGGCG 
               
               
                   
               
               
                 512 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTTTATTGCACAT 
               
               
                   
                   
                 Probe 502 
                 GGTGGTGCAGCTCGT 
               
               
                   
               
               
                 513 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTCTTTTGGTTGT 
               
               
                   
                   
                 Probe 503 
                 CGTGCCCGAGTGCAA 
               
               
                   
               
               
                 514 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTAACAGTAATG 
               
               
                   
                   
                 Probe 504 
                 TTGGCGGTGGTCGCCC 
               
               
                   
               
               
                 515 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTGGTATCGGGC 
               
               
                   
                   
                 Probe 505 
                 GATTTGGATCCGCCAG 
               
               
                   
               
               
                 516 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTTCATTGCCTTG 
               
               
                   
                   
                 Probe 506 
                 TTCGGCTCGTTCGGT 
               
               
                   
               
               
                 517 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTTTGGCGCACC 
               
               
                   
                   
                 Probe 507 
                 AGATCCTGTGATGGCT 
               
               
                   
               
               
                 518 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTTACGCTTACCA 
               
               
                   
                   
                 Probe 508 
                 CACCGAGCACCAGCT 
               
               
                   
               
               
                 519 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTCTACGTTCACG 
               
               
                   
                   
                 Probe 509 
                 CTTTCACCTCCACGC 
               
               
                   
               
               
                 520 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTGTGCCGTTGG 
               
               
                   
                   
                 Probe 510 
                 GCCGAGGAACAGGAAT 
               
               
                   
               
               
                 521 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTCCGGCACCAA 
               
               
                   
                   
                 Probe 511 
                 CCAGACGAGACACCGA 
               
               
                   
               
               
                 522 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTGACGACGGCG 
               
               
                   
                   
                 Probe 512 
                 ACGATCCGGTCTTCAT 
               
               
                   
               
               
                 523 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTATTCGCGATG 
               
               
                   
                   
                 Probe 513 
                 GTGCAGTTCTTGCTCC 
               
               
                   
               
               
                 524 
                 mRNA 
                 Encoding 
                 GATGATGTAGTAGTAAGGGTGACGAAACGA 
               
               
                   
                   
                 Probe 514 
                 CGTTCCAGCGCAGCAT 
               
               
                   
               
            
           
         
       
     
     Example 5. HiPR Swap 
     DNA exchange was used as a method to quickly, specifically, carefully replace the HiPR-FISH readout probes without disturbing encoding and/or amplifier probes. This method is referred to as HiPR-Swap. 
     In the HiPR-Swap method, readout and encoding probes are designed such that the “landing pad” (the region on the encoding probe to which the readout probe binds) is complementary to the readout probe. In some instances, the landing pad sequence is shorter than the readout probe. This would create a single-stranded overhang of the readout probe, as it extends past the end of the landing pad (see  FIG.  8   , line (2)). 
     After a readout probe is bound, an exchange probe can be added to the specimen. The exchange probe is constructed to be of equal length and a perfect reverse complement to the readout probe. When added, the exchange probe seeds a hybridization to the exposed area of the readout probe (see  FIG.  8   , line 3a). Over a short period of time the exchange probe completely hybridizes to the readout probe, thereby removing it from the encoding probe where it can be washed away. Importantly, orthogonal (non-interacting at room temperature to 42° C.) readout and exchange probes can be added simultaneously to reduce assay time (see  FIG.  8   , lines 3b and 3c.) 
     In theory, there is no limit to the number of times the assay can be performed. The maximum number of probes needed is the number of fluorescent probes observable in a single round (for example, 10) multiplied by the number of rounds. For example, if 4 rounds are performed, this will require 40 unique probes each bound with one of 10 fluorescent dyes. This would allow the target multiplexity to be (2{circumflex over ( )}(10)−1){circumflex over ( )}4=1,095,222,947,841 targets. 
     Advantages 
     Thermodynamics models can be applied to understand the extent to which probe swapping is likely to succeed. For example, the Boltzmann factor can be naively implemented to illustrate the improved likelihood of the readout-exchange probe duplex over the readout-encoding probe duplex (false assumption that the system is at equilibrium). 
     The probability of being in a state is given by the distribution: 
     
       
         
           
             
               P 
               ⁡ 
               ( 
               
                 ε 
                 n 
               
               ) 
             
             = 
             
               
                 1 
                 Z 
               
               ⁢ 
               
                 
                   e 
                   
                     
                       - 
                       
                         ε 
                         n 
                       
                     
                     / 
                     
                       k 
                       B 
                     
                     ⁢ 
                     T 
                   
                 
                 . 
               
             
           
         
       
     
     Knowing this, one can find the Boltzmann factor as the ratio of probabilities (P(readout-exchange)/P(readout-encoding)). 
     The Boltzmann factor for various combinations of the readout probes was determined, where the overhang can be 1 to 5 nt from the 3′ or 5′ end (yellow or blue, respectively) and found that the likelihood of being in the readout-exchange state increases dramatically as the length of the overhang increases; the Boltzmann factor can exceed 10,000×. 
     HiPR-Swap, in combination with other technologies, will create a FISH-based assay with the highest multiplexity yet achieved. Its application to spectral barcoding and classification, to the study of microbiomes and bacteria, and its use to profile rRNA and mRNA (and potentially other analytes) make this method an improvement over the prior art. 
     Example 6. HiPR-Swap 
     An experiment was performed where three species of bacteria ( Escherichia coli, Pseudomonas aeruginosa , and  Klebsiella pneumoniae ) were encoded with 18-24 encoding probes, with 15-nt landing pads. Each species was encoded such that they were hybridized with a single, unique bit (or dye). 
     The experiment was performed to (1) show the addition of exchange probes removes readout probes (and thereby fluorescence signal) and (2) following the exchange, new readout probes can be re-hybridized to the specimens without the addition of new encoding probes. 
     The procedure was as follows: Cells were adhered to a coverslip via evaporation. Each species of bacteria was separated from the others using a gasket. The cells were then digested with lysozyme at 37° C. for 30 minutes and washed with 1×PBS at room temperature for 15 minutes. The encoding probe hybridization and readout probe hybridization were performed in a single step. The hybridization buffer was prepared separately for each species (10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, 400 nM of the readout probe, 2 uM per taxa of the encoding probes). The hybridization buffer was then added to the cells at 37° C. for 2 hours. The wash buffer (215 mM NaCl, 20 mM Tris-HCl (pH 8.0), and 5 mM EDTA) was then added to the cells at 30° C. for 15 minutes. The cells were imaged in the wash buffer. The cells were removed from the scope. The exchange buffer was then added to the cells at 37° C. and left overnight. The exchange buffer was prepared separately for each species (10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, and 2 uM of the exchange probe). The wash buffer was added to the cells at 30° C. for 15 minutes. The cells were imaged in the wash buffer. The readout buffer (prepared separately for each species: 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, and 400 nM of the readout probe) was added to the cells and incubated at 37° C. for 2 hours. The wash buffer was added to the cells at 30° C. for 15 minutes. The cells were imaged in the wash buffer. The cells were removed from the scope and stored at 4° C. 
     As evidenced by  FIG.  9   , the results of the experiment showed that each species was properly encoded after the first round (“HiPR-FISH”). Further, each species had their fluorescence signal, and thus readout probes, removed with exchange probes (“Strip”), and the original signal was fully recovered (with correct encoding), with addition of readout probes after the Strip (“Swap”). 
     The encoding, readout, and exchange probes used in this example are shown in Table 5 below. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Encoding, readout, and exchange probes used in Example 6. 
               
            
           
           
               
               
               
            
               
                 SEQ ID NO: 
                 Probe Name 
                 Sequence 
               
               
                   
               
            
           
           
               
               
               
            
               
                 525 
                 Encoding Probe 515 
                 TGGAAGTTAGAGGGTCCTCAGTTAATGATAGT 
               
               
                   
                   
                 GTGTCGATTG 
               
               
                   
               
               
                 526 
                 Encoding Probe 516 
                 TGGAAGTTAGAGGGTGGAGCCTTGGTTTTCCG 
               
               
                   
                   
                 GATTACG 
               
               
                   
               
               
                 527 
                 Encoding Probe 517 
                 TGGAAGTTAGAGGGTGTGTCTCATCTCTGAAA 
               
               
                   
                   
                 ACTTCCCAC 
               
               
                   
               
               
                 528 
                 Encoding Probe 518 
                 TGGAAGTTAGAGGGTGTCACCCCATTAAGAG 
               
               
                   
                   
                 GCTCCGTG 
               
               
                   
               
               
                 529 
                 Encoding Probe 519 
                 TGGAAGTTAGAGGGTCCACGTCAATGAGCAA 
               
               
                   
                   
                 AGGTAAAT 
               
               
                   
               
               
                 530 
                 Encoding Probe 520 
                 TGGAAGTTAGAGGGTGTAAGCTCACAATATGT 
               
               
                   
                   
                 GCATAAA 
               
               
                   
               
               
                 531 
                 Encoding Probe 521 
                 TGGAAGTTAGAGGGTGATACACACACTGATTC 
               
               
                   
                   
                 AGGCAGA 
               
               
                   
               
               
                 532 
                 Encoding Probe 522 
                 TGGAAGTTAGAGGGTAGTCTTGGTTTTCCGGA 
               
               
                   
                   
                 TTTGGGA 
               
               
                   
               
               
                 533 
                 Encoding Probe 523 
                 TGGAAGTTAGAGGGTACCTCAGTTAATGATAG 
               
               
                   
                   
                 TGTGTCGTTT 
               
               
                   
               
               
                 534 
                 Encoding Probe 524 
                 TGGAAGTTAGAGGGTGAGCCTTGGTTTTCCGG 
               
               
                   
                   
                 ATTTCGG 
               
               
                   
               
               
                 535 
                 Encoding Probe 525 
                 TGGAAGTTAGAGGGTGTATCATCTCTGAAAAC 
               
               
                   
                   
                 TTCCGACC 
               
               
                   
               
               
                 536 
                 Encoding Probe 526 
                 TGGAAGTTAGAGGGTGTGCTCAGCCTTGGTTT 
               
               
                   
                   
                 TCCGCTA 
               
               
                   
               
               
                 537 
                 Encoding Probe 527 
                 TGGAAGTTAGAGGGTTGCGTCACCCCATTAAG 
               
               
                   
                   
                 AGGCAGG 
               
               
                   
               
               
                 538 
                 Encoding Probe 528 
                 TGGAAGTTAGAGGGTCATGTCAATGAGCAAA 
               
               
                   
                   
                 GGTATTAAGAA 
               
               
                   
               
               
                 539 
                 Encoding Probe 529 
                 TGGAAGTTAGAGGGTGTAAGCTCACAATATGT 
               
               
                   
                   
                 GCATTAAA 
               
               
                   
               
               
                 540 
                 Encoding Probe 530 
                 TGGAAGTTAGAGGGTGAAACTAACACACACA 
               
               
                   
                   
                 CTGATTGTC 
               
               
                   
               
               
                 541 
                 Encoding Probe 531 
                 TGGAAGTTAGAGGGTCTAAGTTAATGATAGTG 
               
               
                   
                   
                 TGTCGATTG 
               
               
                   
               
               
                 542 
                 Encoding Probe 532 
                 TGGAAGTTAGAGGGTGTGTCTCATCTCTGAAA 
               
               
                   
                   
                 ACTTCCGACC 
               
               
                   
               
               
                 543 
                 Encoding Probe 533 
                 TGGAAGTTAGAGGGTAGGAAGGCACATTCTC 
               
               
                   
                   
                 ATCTCACT 
               
               
                   
               
               
                 544 
                 Encoding Probe 534 
                 TGGAAGTTAGAGGGTCGTCACCCCATTAAGA 
               
               
                   
                   
                 GGCTCGGT 
               
               
                   
               
               
                 545 
                 Encoding Probe 535 
                 TGGAAGTTAGAGGGTGCGTCACCCCATTAAG 
               
               
                   
                   
                 AGGCTAGG 
               
               
                   
               
               
                 546 
                 Encoding Probe 536 
                 TGGAAGTTAGAGGGTCATGTCAATGAGCAAA 
               
               
                   
                   
                 GGTATTATGA 
               
               
                   
               
               
                 547 
                 Encoding Probe 537 
                 TGGAAGTTAGAGGGTTAGGCTCACAATATGTG 
               
               
                   
                   
                 CATTAAA 
               
               
                   
               
               
                 548 
                 Encoding Probe 538 
                 TGGAAGTTAGAGGGTTGACACACACACTGATT 
               
               
                   
                   
                 CAGGGAG 
               
               
                   
               
               
                 549 
                 Encoding Probe 539 
                 AGGTTGAGAATAGGAGAGGCTCAGTAGTTTT 
               
               
                   
                   
                 GGATGCTCA 
               
               
                   
               
               
                 550 
                 Encoding Probe 540 
                 AGGTTGAGAATAGGAAGACGCGTCACTTACG 
               
               
                   
                   
                 TGACACGGC 
               
               
                   
               
               
                 551 
                 Encoding Probe 541 
                 AGGTTGAGAATAGGAGTGGAGGTGCTGGTAA 
               
               
                   
                   
                 CTAAGCTG 
               
               
                   
               
               
                 552 
                 Encoding Probe 542 
                 AGGTTGAGAATAGGACTAGTTTTATGGGATTA 
               
               
                   
                   
                 GCTCCAGGA 
               
               
                   
               
               
                 553 
                 Encoding Probe 543 
                 AGGTTGAGAATAGGAGAGGAAAGTTCTCAGC 
               
               
                   
                   
                 ATGTCTTC 
               
               
                   
               
               
                 554 
                 Encoding Probe 544 
                 AGGTTGAGAATAGGAACACCCATGCTCGGCA 
               
               
                   
                   
                 CTTCTCCC 
               
               
                   
               
               
                 555 
                 Encoding Probe 545 
                 AGGTTGAGAATAGGACGCGGTGTTTTTCACAC 
               
               
                   
                   
                 CCATACA 
               
               
                   
               
               
                 556 
                 Encoding Probe 546 
                 AGGTTGAGAATAGGATGGCCAGAGTGATACA 
               
               
                   
                   
                 TGAGGGCG 
               
               
                   
               
               
                 557 
                 Encoding Probe 547 
                 AGGTTGAGAATAGGATGGCTATCTCCGAGCTT 
               
               
                   
                   
                 GATTTCG 
               
               
                   
               
               
                 558 
                 Encoding Probe 548 
                 AGGTTGAGAATAGGAGGCACACAGGAAATTC 
               
               
                   
                   
                 CACCAAGG 
               
               
                   
               
               
                 559 
                 Encoding Probe 549 
                 AGGTTGAGAATAGGAAAGATCCAACTTGCTG 
               
               
                   
                   
                 AACCAGGA 
               
               
                   
               
               
                 560 
                 Encoding Probe 550 
                 AGGTTGAGAATAGGATGCGTCACCTAACAAG 
               
               
                   
                   
                 TAGGCAGG 
               
               
                   
               
               
                 561 
                 Encoding Probe 551 
                 AGGTTGAGAATAGGACGTGTATTAACTTACTG 
               
               
                   
                   
                 CCCTTCGAG 
               
               
                   
               
               
                 562 
                 Encoding Probe 552 
                 AGGTTGAGAATAGGAACAAGACAAAGTTTCT 
               
               
                   
                   
                 CGTGCAGG 
               
               
                   
               
               
                 563 
                 Encoding Probe 553 
                 AGGTTGAGAATAGGAAAACTTCAAAGATCCT 
               
               
                   
                   
                 TTCGCCAT 
               
               
                   
               
               
                 564 
                 Encoding Probe 554 
                 AGGTTGAGAATAGGAGCACGCTAAAATCAAT 
               
               
                   
                   
                 GAAGCTATT 
               
               
                   
               
               
                 565 
                 Encoding Probe 555 
                 AGGTTGAGAATAGGACGATCTGATAGCGTGA 
               
               
                   
                   
                 GGTCCCTT 
               
               
                   
               
               
                 566 
                 Encoding Probe 556 
                 AGGTTGAGAATAGGAATAATTCAGTACAAGA 
               
               
                   
                   
                 TACCTAGGAAT 
               
               
                   
               
               
                 567 
                 Encoding Probe 557 
                 AGGTTGAGAATAGGAAGGCGCTGAATCCAGG 
               
               
                   
                   
                 AGCAACGA 
               
               
                   
               
               
                 568 
                 Encoding Probe 558 
                 AGGTTGAGAATAGGACAAAACGCTCTATGAT 
               
               
                   
                   
                 CGTCAATA 
               
               
                   
               
               
                 569 
                 Encoding Probe 559 
                 AGGTTGAGAATAGGAGCAGTGTTTTTCACACC 
               
               
                   
                   
                 CATTGTGCA 
               
               
                   
               
               
                 570 
                 Encoding Probe 560 
                 AGGTTGAGAATAGGACTGCGATCGGTTTTATG 
               
               
                   
                   
                 GGATATC 
               
               
                   
               
               
                 571 
                 Encoding Probe 561 
                 AGGTTGAGAATAGGAGGATCGACGTGTCTGT 
               
               
                   
                   
                 CTCGCTCA 
               
               
                   
               
               
                 572 
                 Encoding Probe 562 
                 AGGTTGAGAATAGGAGGTGCAGTAACCAGAA 
               
               
                   
                   
                 GTACACCT 
               
               
                   
               
               
                 573 
                 Encoding Probe 563 
                 GGTGTAGGGAGTAAAACCTCTTCGACTGGTCT 
               
               
                   
                   
                 CAGCAGG 
               
               
                   
               
               
                 574 
                 Encoding Probe 564 
                 GGTGTAGGGAGTAAATGCAATCGATGAGGTT 
               
               
                   
                   
                 ATTAACCTGTA 
               
               
                   
               
               
                 575 
                 Encoding Probe 565 
                 GGTGTAGGGAGTAAACATCAGTCACACCCGA 
               
               
                   
                   
                 AGGTGCTAGG 
               
               
                   
               
               
                 576 
                 Encoding Probe 566 
                 GGTGTAGGGAGTAAAGCAATCGATGAGGTTA 
               
               
                   
                   
                 TTAACCTGTA 
               
               
                   
               
               
                 577 
                 Encoding Probe 567 
                 GGTGTAGGGAGTAAACATCAGTCACACCCGA 
               
               
                   
                   
                 AGGTGCAGG 
               
               
                   
               
               
                 578 
                 Encoding Probe 568 
                 GGTGTAGGGAGTAAAATGAGTCACACCCGAA 
               
               
                   
                   
                 GGTGCTAGG 
               
               
                   
               
               
                 579 
                 Encoding Probe 569 
                 GGTGTAGGGAGTAAATCCCTTCACCTACACAC 
               
               
                   
                   
                 CAGCGACG 
               
               
                   
               
               
                 580 
                 Encoding Probe 570 
                 GGTGTAGGGAGTAAATCCCTTCACCTACACAC 
               
               
                   
                   
                 CAGCCAC 
               
               
                   
               
               
                 581 
                 Encoding Probe 571 
                 GGTGTAGGGAGTAAATGACCGCAACCCCGGT 
               
               
                   
                   
                 GAGGGCGG 
               
               
                   
               
               
                 582 
                 Encoding Probe 572 
                 GGTGTAGGGAGTAAAAGAGACTGGTCTCAGC 
               
               
                   
                   
                 TCCACGGC 
               
               
                   
               
               
                 583 
                 Encoding Probe 573 
                 GGTGTAGGGAGTAAAATGAGTCACACCCGAA 
               
               
                   
                   
                 GGTGCAGG 
               
               
                   
               
               
                 584 
                 Encoding Probe 574 
                 GGTGTAGGGAGTAAATGCGTCACACCCGAAG 
               
               
                   
                   
                 GTGCTAGG 
               
               
                   
               
               
                 585 
                 Encoding Probe 575 
                 GGTGTAGGGAGTAAAGTGCTCAGCCTTGATTA 
               
               
                   
                   
                 TCCGCTA 
               
               
                   
               
               
                 586 
                 Encoding Probe 576 
                 GGTGTAGGGAGTAAACCACGTCAATCGATGA 
               
               
                   
                   
                 GGTTAAAT 
               
               
                   
               
               
                 587 
                 Encoding Probe 577 
                 GGTGTAGGGAGTAAAAATAACCTCATCGCCTT 
               
               
                   
                   
                 CCTCAGG 
               
               
                   
               
               
                 588 
                 Encoding Probe 578 
                 GGTGTAGGGAGTAAACCCACGTCAATCGATG 
               
               
                   
                   
                 AGGTTTAA 
               
               
                   
               
               
                 589 
                 Encoding Probe 579 
                 GGTGTAGGGAGTAAACATCAGTCACACCCGA 
               
               
                   
                   
                 AGGTGGAG 
               
               
                   
               
               
                 590 
                 Encoding Probe 580 
                 GGTGTAGGGAGTAAACCCTTCACCTACACACC 
               
               
                   
                   
                 AGCGACG 
               
               
                   
               
               
                 4 
                 Readout Probe 4 
                 /5PacificGreenN/ACCCTCTAACTTCCATCACA 
               
               
                   
               
               
                 6 
                 Readout Probe 6 
                 /5Atto610N/TTTACTCCCTACACCTCCAA 
               
               
                   
               
               
                 8 
                 Readout Probe 8 
                 /5DyLight-510-LS/ 
               
               
                   
                   
                 TCCTATTCTCAACCTAACCT/3DyLight-510-LS/ 
               
               
                   
               
               
                 591 
                 Exchange Probe 1 
                 TGTGATGGAAGTTAGAGGGT 
               
               
                   
               
               
                 592 
                 Exchange Probe 2 
                 TTGGAGGTGTAGGGAGTAAA 
               
               
                   
               
               
                 593 
                 Exchange Probe 3 
                 AGGTTAGGTTGAGAATAGGA 
               
               
                   
               
            
           
         
       
     
     Example 7. Timescale Determination the Exchange Reaction in HiPR-Swap 
     The experiment was continued after 5 days in the same samples as described in Example 6. To determine the timescale of the exchange reaction, the reaction was performed for 1 hour. 
     The experiment was performed to show that the stripping of readout probes can be achieved within 1 hour, as opposed to a longer period of time, such as over 12 hours. 
     The procedure was as follows. The cells were removed from the 4° C. refrigerator after 5 days and imaged in the wash buffer. The cells were removed from the scope and the exchange buffer was added to the cells at 37° C. for 1 hour. The wash buffer was then added to the cells at 30° C. for 15 minutes and the cells were imaged in the wash buffer. The encoding, readout, and exchange probes used in this example are shown in Table 5. 
     As can be seen in  FIG.  10   , the experiment showed that the fluorescence signal from  P. aeruginosa  and  K. pneumoniae  did not degrade significantly after 5 days. The fluorescence signal from  E. coli  had degraded significantly due to rapid photobleaching and instability of the Atto-390 dye in the wash buffer (“After 5 days”). Each species had most of their readout probes removed within a span of 1 hour (“Strip—1 hr”). There is a small fluorescence signal left after 1 hour. Therefore, the whole exchange reaction can be completed within 1.5-2 hours or less. 
     Example 8. Recovery Of Signal With Different Readout Probes in HiPR-Swap 
     This experiment was performed to show the sequential repeatability of the HiPR-Swap method and continues from Example 7. 
     After stripping the readout probes for 1 hour, the stripping reaction was continued overnight to remove the remaining readout probes. Following this, each species was encoded with the readout probes that correspond to their respective readout pads but tagged with the same dye (Alexa-488). 
     The procedure was as follows. The exchange buffer was added to the cells at 37° C. and left overnight. The wash buffer was then added to the cells at 30° C. for 15 minutes and the cells were imaged in the wash buffer. The cells were removed from the scope. A readout buffer was prepared separately for each species containing one of the following probes: R4-488, R6-488, R8-488. The readout buffer was then added to the cells and incubated at 37° C. for 2 hours. The wash buffer was added to the cells at 30° C. for 15 minutes and the cells were imaged in the wash buffer. 
     As shown in  FIG.  11   , the experiment showed that the fluorescent signal was completely removed from each species (“Strip-overnight”) and the fluorescence signal was recovered with the encoded color (green, not shown) after adding the readout probes (“Swap—R #-488”). 
     Overall, these results demonstrate the full two cycles of HiPR-swap assay with robust removal and re-hybridization of the readout probes. 
     The R4-488, R6-488, R8-488 probes are shown in Table 6 below. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 488 Readout Probes. 
               
            
           
           
               
               
               
            
               
                 SEQ ID 
                   
                   
               
               
                 NO: 
                 Probe Name 
                 Sequence (in 5′ to 3′ order) 
               
               
                   
               
               
                 594 
                 R4-488 
                 /5Alex488N/ACCCTCTAACTTCCATCACA 
               
               
                   
               
               
                 595 
                 R6-488 
                 /5Alex488N/TTTACTCCCTACACCTCCAA 
               
               
                   
               
               
                 596 
                 R8-488 
                 /5Alex488N/TCCTATTCTCAACCTAACCT 
               
               
                   
               
            
           
         
       
     
     Example 9. Single-Step Probe Exchange and New Readout Addition 
     As shown in Examples 6-7, the readout probes can be removed (stripped) and replaced (swapped) in two subsequent steps. As long as the second round of readout probes differs from the first set that is being removed with exchange probes, the strip and swap can be performed in a single step. 
     Single-step HiPR-Swap and two-step HiPR-Swap was performed on a single slide with neighboring wells. In both wells, a mixture of  E. coli  and  P. aeruginosa  cells was adhered to the surface. 
     Round 1: In the first round for both wells, the taxa encoding probes for both species (including EUB which will serve as a tool to segment cells for analysis) were added and readout probes only for  E. coli . The encoding and readout hybridization reactions were performed in a single step. Both wells were imaged following the first round of encoding and readout. 
     Round 2: In round two of the single-step well, the readout probes from  E. coli  were stripped and swapped with the readout probes for  P. aeruginosa . For the two-step well, only the readout probes were stripped from  E. coli . Both wells were imaged following this hybridization step. 
     Round 3: In round three of the single-step well, the readout probes from  P. aeruginosa  were stripped and swapped with the readout probes for  E. coli . For the two-step well, only the readout probes were stripped from  P. aeruginosa . Both wells were imaged following this hybridization step. 
     The experiment was conducted as follows mixtures of cells were adhered to a coverslip via evaporation. The cells were digested with lysozyme at 37° C. for 30 minutes. The cells were washed with 1×PBS at room temperature for 15 minutes. 
     Round 1: The encoding probe hybridization and readout probe hybridization were performed in a single step. The hybridization buffer was prepared as follows for both the wells: 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, 2 uM per taxa of the encoding probes, 400 nM of the  Eubacterium  probe, and 400 nM of the readout probe for  E. coli . The hybridization buffer was added to the cells at 37° C. for 2 hours. The wash buffer (215 mM NaCl, 20 mM Tris-HCl (pH 8.0); and 5 mM EDTA) was added to the cells at 30° C. for 15 minutes. All wells were filled in excess with 2×SSC. A glass coverslip was placed on top of the wells to minimize evaporation. The cells were imaged under 2×SSC. Then, the cells were removed from the scope. The cells were washed with wash buffer for 1 min at RT. The cells were stored overnight in the wash buffer at 4° C. 
     Round 2: The exchange buffers were prepared separately for each well. Well: Single Step: 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, 6 uM of the exchange probe for  E. coli , and 400 nM of the readout probes for  P. aeruginosa . Well: Two Step: 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, and 6 uM of the exchange probe for  E. coli.    
     The exchange buffers were added to the cells at 37° C. for 2 hours. The wash buffer was added to the cells at 30° C. for 15 minutes. All wells were filled in excess with 2×SSC. A glass coverslip was placed on top of the wells to minimize evaporation. The cells were imaged under 2×SSC. The cells were removed from the scope. The cells were washed with wash buffer for 1 min at RT. 
     Round 3: The exchange buffers were prepared separately for each well. Well: Single Step: 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, 6 uM of the exchange probe for  P. aeruginosa , and 400 nM of the readout probes for  E. coli . Well: Two Step: 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, and 400 nM of the readout probes probe for  P. aeruginosa . The exchange buffers were added to the cells at 37° C. for 2 hours. The wash buffer was added to the cells at 30° C. for 15 minutes. All wells were filled in excess with 2×SSC. A glass coverslip was placed on top of the wells to minimize evaporation. The cells were imaged under 2×SSC. The encoding, readout, and exchange probes used in this example are shown in Table 5. 
     The single-step strip and swap reaction works equally well as the two-step reaction. This enables us to perform multiple rounds of HiPR-Swap (for example, at least 3 rounds for 30 bit barcode) in less than 12 hours. 
     As shown in  FIG.  12   , with single-step condition, successful demonstration of HiPR-Swap was shown up to 3 rounds. 
     In single step condition,  E. coli  in round 1 is dimmer than the  E. coli  in round 3. This is likely because of the inefficient binding of readout probes to the readout pads in the first round of encoding/readout, where single step encoding and readout was used to perform HiPR-FISH. An addition of pre-hybridization incubation step before encoding/readout step can improve the binding efficiency of readout probes in round 1. 
     Example 10. Realtime Measurement of HiPR-Swap Using Single-Step Strip and Swap Reaction 
     The single step strip and swap reaction was shown to work equally well as the two-step reaction. In this example, the single-step reaction was used to measure the stripping and swapping of the probes in real time. 
     Single-step HiPR-Swap was performed with a mixture of  E. coli  and  P. aeruginosa  cells. 
     Round 1: In the first round, the taxa encoding probes were added for both species and readout probes only for  E. coli . The encoding and readout hybridization reactions were performed in a single step. The cells were imaged after this hybridization step. 
     Round 2: In the second round, the cells were placed under the microscope. the readout probes were stripped from  E. coli  and swapped with the readout probes for  P. aeruginosa . Images were acquired while the stripping and swapping reaction was undergoing. 
     The following was performed in this example. Mixture of cells were adhered to a coverslip via evaporation. The cells were digested with lysozyme at 37° C. for 30 minutes and washed with 1×PBS at room temperature for 15 minutes. The pre-hybridization buffer (10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS) was added to the cells at 37° C. for 30 mins. 
     Round 1: The encoding probe hybridization and readout probe hybridization were performed in a single step. The hybridization buffer (both wells; 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, 2 μM per taxa of the encoding probes, and 400 nM of the readout probe for  E. coli ) was added to the cells at 37° C. for 2 hours. The wash buffer (215 mM NaCl, 20 mM Tris-HCl (pH 8.0); and 5 mM EDTA) was added to the cells at 30° C. for 15 minutes. The cells were placed on the microscope and imaged under the wash buffer before acquiring the timelapse. 
     Round 2: The wash buffer was removed and the well was filled with the exchange buffer (10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, 50 nM of the exchange probe for  E. coli , and 25 nM of the readout probes for  P. aeruginosa ) under the microscope. The timelapse was started and images were acquired at a 15 seconds interval. The encoding, readout, and exchange probes used in this example are shown in Table 5. 
     As shown in  FIG.  13   , the real time stripping and swapping of the readout probes in the mixture of two species was demonstrated. 
     To capture the kinetics of this reaction, the reaction was purposefully slowed down dramatically by using a very low concentration of the exchange probes (50 nM) and the readout probes (25 nM). At higher concentrations, such as 2 uM for exchange probes and 400 nM for readout probes, in here the strip and swap reactions can be completed within a few minutes. 
     Notably, with the addition of the pre-hybridization step, the binding efficiency of the readout probes in the first round improved dramatically, as evident from the intensity of the “before” image in timelapse. 
     Example 11.  E. coli  Encoding with 30-Bit Barcode, and Measured in 3 Rounds 
     To show the full potential of using HiPR-Swap towards increasing the multiplexity of HiPR-FISH related assays, including HIPR-FAST and HIPR-cycle, the ability to identify over 1 billion taxa (or other targets; 1023{circumflex over ( )}3) in about 12 hours was shown.  FIG.  14    shows a schematic for this example and  FIG.  15    shows an overview of HiPR-Swap as in this example. 
     This example was performed with  E. coli  bacteria bound to the coverslips in three wells. The bacteria in each well was encoded with a unique 30-bit barcode (e.g. 0110001000-0100100111-1101001000). The 30-bit experiment was performed in three rounds using HIPR-Swap, with each round containing up to 10-bits. A fourth round was added for error correction by going back to the same readouts as round 1. 
     Round 1: In the first round, the taxa encoding probes for bacteria were added in each well and incubated overnight. The first set of readout probes were added in each well. The cells were imaged after this hybridization step. 
     Round 2: In the second round, the first set of exchange probes to strip readout probes of round 1 was added, and second set of readout probes in each well. The cells were imaged after this hybridization step. 
     Round 3: In the third round, the second set of exchange probes to strip readout probes of round 2 was added, and third set of readout probes in each well. The cells were imaged after this hybridization step. 
     Round 4: In the fourth round, the third set of exchange probes to strip readout probes of round 3 were added, and first set of readout probes in each well. This was done to go back to the same sets of readout probes as used in round 1. The cells were imaged after this hybridization step. 
     The single step HiPR-Swap protocol was utilized as follows: cells were adhered to a coverslip via evaporation. The cells were digested with lysozyme at 37° C. for 30 minutes. The cells were washed with 1×PBS at room temperature for 15 minutes. 
     Round 1: The encoding buffer was prepared separately for each well as follows: 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, 2 μM each of encoding probes combination (C #, where #=readout probe #) as described below—
         Well 1: C11+C13+C16+C18   Well 2: C12+C15+C21   Well 3: C13+C17+C19
 
A combination of encoding probes, C #, encompasses 24 encoding probes (as shown in Table 7 below) each concatenated to readout landing pads sequences corresponding to a specific readout probe number (#). For example, Combination 11 (C11) corresponds to 24 encoding probes each concatenated to landing pad sequence 11: TTAATATGGGTAGTTGGG (SEQ ID NO.: 1810). The landing pad sequence is partially complementary to the sequence of Readout Probe 11 (SEQ ID NO.: 597).
       

     The encoding buffer was added to the cells at 37° C. and incubated overnight. The wash buffer was prepared as 215 mM NaCl, 20 mM Tris-HCl (pH 8.0), 5 mM EDTA. The wash buffer was added to the cells at 42° C. for 15 minutes. The readout buffer was prepared as follows 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, and 400 nM each of readout probe 11, 12, 13. The readout buffer was added to the cells at 37° C. for 1 hour. The wash buffer was added to the cells at 42° C. for 15 minutes. All wells were filled in excess with 2×SSCT. A glass coverslip was placed on top of the wells to minimize evaporation. The cells were imaged under 2×SSCT. The cells were removed from the scope. The cells were washed with 2×SSC for 1 min at RT. 
     Round 2: The exchange buffer for round 2 was prepared as follows 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, 10 uM each of exchange probe 5, 8, and 10, 400 nM each of readout probe 14-17. The exchange buffer was added to the cells at 37° C. for 1 hour. The wash buffer was added to the cells at 42° C. for 15 minutes. All wells were filled in excess with 2×SSCT. A glass coverslip was placed on top of the wells to minimize evaporation. The cells were imaged under 2×SSCT. The cells were removed from the scope. The cells were washed with 2×SSC for 1 min at RT. 
     Round 3: The exchange buffer for round 3 was prepared as follows: 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, 10 uM each of exchange probe 14, 15, 17, 18, 400 nM each of readout probe 18-21. The exchange buffer was added to the cells at 37° C. for 1 hour. The wash buffer was added to the cells at 42° C. for 15 minutes. All wells were filled in excess with 2×SSCT. A glass coverslip was placed on top of the wells to minimize evaporation. The cells were imaged under 2×SSCT. The cells were removed from the scope. The cells were washed with 2×SSC for 1 min at RT. 
     Round 4: The exchange buffer for round 4 was prepared as follows: 10% ethylene carbonate, 10% dextran sulfate, 2×SSC, 5×Denhardt&#39;s solution, 0.01% SDS, 10 uM each of exchange probe 24, 25, 28, 30, 400 nM each of readout probe 11, 12, and 13. The exchange buffer was added to the cells at 37° C. for 1 hour. The wash buffer was added to the cells at 42° C. for 15 minutes. All wells were filled in excess with 2×SSCT. A glass coverslip was placed on top of the wells to minimize evaporation. The cells were imaged under 2×SSCT. 
       FIGS.  16 A- 16 B  shows a summary of classification accuracy for this example. As shown in  FIG.  17   , bacteria fluorescence matches the expected barcode. In each well a mask for the most abundant barcode applied to the maximum spectral projection. Fluorescent bacteria only appear in channels corresponding to the “1” bit. 
     Microscopy 
     As indicated above, in each round, imaging using confocal microscopy (Zeiss i880 confocal microscope) with emission collected was collected on a spectral detector between roughly the excitation wavelength and 693 nm in 8.9 nm bins. A Plan-Apochromat 63×/1.4 Oil DIC M27 was used and collected data as 2000×2000 pixel images (134.95 μm×134.95 μm). The laser settings for the example were as shown in Table 6 below. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Laser settings 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Pixel 
                   
                   
                   
                   
                   
                   
               
               
                 Laser 
                   
                 Pinhole 
                 Laser 
                 Dwell 
               
               
                 Excitation 
                   
                 Size 
                 Power 
                 Time 
                 Bit 
                 Scanning 
                 Scanning 
                 Master 
                 Digital 
                 Digital 
               
               
                 (nm) 
                 Laser 
                 (nm) 
                 (%) 
                 (μsec) 
                 Depth 
                 Direction 
                 Repeats 
                 Gain 
                 Offset 
                 Gain 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 488 
                 Argon 
                 56.0 
                 0.5 
                 2.1 
                 16-bit 
                 Bidirectional 
                 4 
                 800 
                 0 
                 1 
               
               
                 514 
                 Argon 
                 58.0 
                 1.5 
                 2.1 
                 16-bit 
                 Bidirectional 
                 4 
                 800 
                 0 
                 1 
               
               
                 561 
                 DPSS 
                 60.0 
                 0.125 
                 2.1 
                 16-bit 
                 Bidirectional 
                 4 
                 800 
                 0 
                 1 
               
               
                   
                 561-10 
               
               
                 633 
                 HeNe633 
                 64.0 
                 1.5 
                 2.1 
                 16-bit 
                 Bidirectional 
                 4 
                 800 
                 0 
                 1 
               
               
                   
               
            
           
         
       
     
     The encoding, readout, and exchange probes used in this example are shown in table 7 below. 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Encoding, readout, and exchange probes used in Example 11 
               
            
           
           
               
               
               
            
               
                 SEQ ID NO: 
                 Probe Name 
                 Sequence (in 5′ to 3′ order) 
               
               
                   
               
            
           
           
               
               
               
            
               
                 597 
                 Readout Probe 11 
                 /5Alex532/CCCAACTACCCATATTAACACACCC 
               
               
                   
               
               
                 598 
                 Readout Probe 12 
                 /56-ROXN/CCCTTCTCACTAAATTCCAACACCC 
               
               
                   
               
               
                 599 
                 Readout Probe 13 
                 /5Alex647N/CACCCTCATATCTATTACCCTCCCA 
               
               
                   
               
               
                 600 
                 Readout Probe 14 
                 /5Alex488N/TCCCTCCTTACTATTACACTCACCC 
               
               
                   
               
               
                 601 
                 Readout Probe 15 
                 /5Alex532/CATCCCTCCTTATTATCCTCATCCC 
               
               
                   
               
               
                 602 
                 Readout Probe 16 
                 /5Alex546N/CCCTTCTACTACTTCCATACATCCC 
               
               
                   
               
               
                 603 
                 Readout Probe 17 
                 /56-ROXN/CCCTTCTAATCCTATACACTCACCC 
               
               
                   
               
               
                 604 
                 Readout Probe 18 
                 /5Alex488N/CCCATCTCTCTAATTCTACTCCACC 
               
               
                   
               
               
                 605 
                 Readout Probe 19 
                 /5Alex532/TCCCTCCTCTTAATACATCCTCCTC 
               
               
                   
               
               
                 606 
                 Readout Probe 20 
                 /56-ROXN/CATCCCTACTTACTTATCCTCCACC 
               
               
                   
               
               
                 607 
                 Readout Probe 21 
                 /5Alex647N/CCCTTCTCCATAACTATACCCTTCC 
               
               
                   
               
               
                 608 
                 Exchange Probe 5 
                 GGGTGTGTTAATATGGGTAGTTGGG 
               
               
                   
               
               
                 609 
                 Exchange Probe 8 
                 GGGTGTTGGAATTTAGTGAGAAGGG 
               
               
                   
               
               
                 610 
                 Exchange Probe 10 
                 TGGGAGGGTAATAGATATGAGGGTG 
               
               
                   
               
               
                 611 
                 Exchange Probe 14 
                 GGGTGAGTGTAATAGTAAGGAGGGA 
               
               
                   
               
               
                 612 
                 Exchange Probe 15 
                 GGGATGAGGATAATAAGGAGGGATG 
               
               
                   
               
               
                 613 
                 Exchange Probe 17 
                 GGGATGTATGGAAGTAGTAGAAGGG 
               
               
                   
               
               
                 614 
                 Exchange Probe 18 
                 GGGTGAGTGTATAGGATTAGAAGGG 
               
               
                   
               
               
                 615 
                 Exchange Probe 24 
                 GGTGGAGTAGAATTAGAGAGATGGG 
               
               
                   
               
               
                 616 
                 Exchange Probe 25 
                 GAGGAGGATGTATTAAGAGGAGGGA 
               
               
                   
               
               
                 617 
                 Exchange Probe 28 
                 GGTGGAGGATAAGTAAGTAGGGATG 
               
               
                   
               
               
                 618 
                 Exchange Probe 30 
                 GGAAGGGTATAGTTATGGAGAAGGG 
               
               
                   
               
               
                 619 
                 Encoding Probe 581 
                 *CCTCAGTTAATGATAGTGTGTCGATTG 
               
               
                   
               
               
                 620 
                 Encoding Probe 582 
                 *GGAGCCTTGGTTTTCCGGATTACG 
               
               
                   
               
               
                 621 
                 Encoding Probe 583 
                 *GTGTCTCATCTCTGAAAACTTCCCAC 
               
               
                   
               
               
                 622 
                 Encoding Probe 584 
                 *GTCACCCCATTAAGAGGCTCCGTG 
               
               
                   
               
               
                 623 
                 Encoding Probe 585 
                 *CCACGTCAATGAGCAAAGGTAAAT 
               
               
                   
               
               
                 624 
                 Encoding Probe 586 
                 *GTAAGCTCACAATATGTGCATAAA 
               
               
                   
               
               
                 625 
                 Encoding Probe 587 
                 *GATACACACACTGATTCAGGCAGA 
               
               
                   
               
               
                 626 
                 Encoding Probe 588 
                 *AGTCTTGGTTTTCCGGATTTGGGA 
               
               
                   
               
               
                 627 
                 Encoding Probe 589 
                 *ACCTCAGTTAATGATAGTGTGTCGTTT 
               
               
                   
               
               
                 628 
                 Encoding Probe 590 
                 *GAGCCTTGGTTTTCCGGATTTCGG 
               
               
                   
               
               
                 629 
                 Encoding Probe 591 
                 *GTATCATCTCTGAAAACTTCCGACC 
               
               
                   
               
               
                 630 
                 Encoding Probe 592 
                 *GTGCTCAGCCTTGGTTTTCCGCTA 
               
               
                   
               
               
                 631 
                 Encoding Probe 593 
                 *TGCGTCACCCCATTAAGAGGCAGG 
               
               
                   
               
               
                 632 
                 Encoding Probe 594 
                 *CATGTCAATGAGCAAAGGTATTAAGAA 
               
               
                   
               
               
                 633 
                 Encoding Probe 595 
                 *GTAAGCTCACAATATGTGCATTAAA 
               
               
                   
               
               
                 634 
                 Encoding Probe 596 
                 *GAAACTAACACACACACTGATTGTC 
               
               
                   
               
               
                 635 
                 Encoding Probe 597 
                 *CTAAGTTAATGATAGTGTGTCGATTG 
               
               
                   
               
               
                 636 
                 Encoding Probe 598 
                 *GTGTCTCATCTCTGAAAACTTCCGACC 
               
               
                   
               
               
                 637 
                 Encoding Probe 599 
                 *AGGAAGGCACATTCTCATCTCACT 
               
               
                   
               
               
                 638 
                 Encoding Probe 600 
                 *CGTCACCCCATTAAGAGGCTCGGT 
               
               
                   
               
               
                 639 
                 Encoding Probe 601 
                 *GCGTCACCCCATTAAGAGGCTAGG 
               
               
                   
               
               
                 640 
                 Encoding Probe 602 
                 *CATGTCAATGAGCAAAGGTATTATGA 
               
               
                   
               
               
                 641 
                 Encoding Probe 603 
                 *TAGGCTCACAATATGTGCATTAAA 
               
               
                   
               
               
                 642 
                 Encoding Probe 604 
                 *TGACACACACACTGATTCAGGGAG 
               
               
                   
               
               
                 1810 
                 Landing Pad 11 
                 TTAATATGGGTAGTTGGG- 
               
               
                   
               
               
                 1811 
                 Landing Pad 12 
                 GGAATTTAGTGAGAAGGG- 
               
               
                   
               
               
                 1812 
                 Landing Pad 13 
                 GTAATAGATATGAGGGTG- 
               
               
                   
               
               
                 1813 
                 Landing Pad 14 
                 TGTAATAGTAAGGAGGGA- 
               
               
                   
               
               
                 1814 
                 Landing Pad 15 
                 GGATAATAAGGAGGGATG- 
               
               
                   
               
               
                 1815 
                 Landing Pad 16 
                 ATGGAAGTAGTAGAAGGG- 
               
               
                   
               
               
                 1816 
                 Landing Pad 17 
                 TGTATAGGATTAGAAGGG- 
               
               
                   
               
               
                 1817 
                 Landing Pad 18 
                 TAGAATTAGAGAGATGGG- 
               
               
                   
               
               
                 1818 
                 Landing Pad 19 
                 ATGTATTAAGAGGAGGGA- 
               
               
                   
               
               
                 1819 
                 Landing Pad 20 
                 GATAAGTAAGTAGGGATG- 
               
               
                   
               
               
                 1820 
                 Landing Pad 21 
                 TATAGTTATGGAGAAGGG- 
               
               
                   
               
               
                 The asterisk (*) represents the concatenated landing pad sequence for each combination (C#). For instance, in C11, each of the encoding probes 581-604 has the sequence of landing pad 11 appended to its 3′ end. For example, Encoding Probe 581 when present in C11, would have a sequence of TTAATATGGGTAGTTGGGCCTCAGTTAATGATAGTGTGTCGATTG (SEQ ID NO: 1821) corresponding to Landing Pad 11 + Encoding Probe 581 as shown in the table. For C12, each of the encoding probes 581-604 has the sequence of landing pad 12 appended to its 3′ end, and so on. The dash (-) on each landing pad sequence represents the point of attachment to the encoding probe. 
               
            
           
         
       
     
     Example 12. Phylum-Level Swap in Tissue Samples 
     To examine the ability to perform HiPR-Swap on a tissue specimen (colon of a healthy mouse) probes were designed to perform a simple taxon identification experiment, barcoding the six most abundant bacteria phyla with either one or two readout probes, such that each readout probe was only present in one of three imaging rounds. As shown in  FIG.  18   , we identified each phylum targeted (Bacteroidota, Verrucomicrobia, Actinobacteria, Firmicutes, Mycoplasmatota, and Proteobacteria). The two most abundant taxa were confirmed to be Firmicutes and Bacteroidota, as expected. Taxonomy of each segmented microbe was classified the across each round of imaging, finding that it was possible to accurately identify over 90% of fluorescently labeled bacteria in each image, and deriving similar abundance measurements for taxa labeled in different rounds (e.g. roughly 50% of bacteria identified as Bacteroidota in both rounds 1 and 3). 
     Phylum-level swap protocol: OCT (optimal cutting temperature)-embedded formalin-fixed tissue was sectioned at 10-micron thickness onto circular glass coverslips made for Bioptechs FCS2 flow cell. The tissue was covered with 2% formaldehyde for two hours at room temperature to fix the sample. The sample was washed by removing the buffer and replacing it with 1×PBS for 5 minutes (this was repeated two more times). The fixed tissue specimen was stored in 70% ethanol at 4° C. overnight. The following buffers were prepared:
         Encoding buffer: Encoding probes (204 per taxa); 2×sodium chloride sodium citrate (SSC), 5×Denhardt&#39;s solution, 10% dextran sulfate, 10% ethylene carbonate, and 0.01% sodium dodecyl sulfate (SDS)   Round 1 readout buffer: 400 nM each of readout probes: 11+13+9; 2×sodium chloride sodium citrate (SSC), 5×Denhardt&#39;s solution, 10% dextran sulfate, 10% ethylene carbonate, and 0.01% sodium dodecyl sulfate (SDS)   Round 2 readout buffer: 10 uM each of exchange probe: 5+10, 400 nM each of readout probes 14+16+17, 2×sodium chloride sodium citrate (SSC), 5×Denhardt&#39;s solution, 10% dextran sulfate, 10% ethylene carbonate, and 0.01% sodium dodecyl sulfate (SDS)   Round 3 readout buffer: 10 uM each of exchange probe: 14+17+18, 400 nM each of readout probes: 19-21, 2×sodium chloride sodium citrate (SSC), 5×Denhardt&#39;s solution, 10% dextran sulfate, 10% ethylene carbonate, 0.01% sodium dodecyl sulfate (SDS)   Wash buffer: 215 mM NaCl, 20 mM Tris-HCl (pH 7.5), 5 mM EDTA       

     Place 10 mg/mL lysozyme to completely cover the specimen and incubate for 30 minutes at 37° C. in a humidified chamber. Wash the specimen with 1×PBS for 15 minutes at room temperature. Dry the specimen by submerging it in 100% ethanol and allowing it to air dry. Place the coverslip on the FCS2 flow cell (Bioptechs) and assemble. Place the flow cell assembly on the microscope stage (Zeiss i880 confocal). Connect the flow cell input port to the Aria Automated Perfusion System (Fluigent). Calibrate the Aria Automated Perfusion System using DI water. Load encoding, readout buffers, wash buffer, 1×PBS buffer, 5×SSC+DAPI buffer (40 ng/mL DAPI in 5×SSC), and 2×SSC buffer into Aria Automated Perfusion System at the desired reservoir locations. Execute the following sequence on the Aria:
         a. Incubate the specimen with 1×PBS at room temperature for 15 minutes.   b. Incubate the specimen in the Encoding buffer at 37° C. for 2 hours.   c. Incubate the specimen in the Wash buffer at 42° C. for 15 minutes.   d. Incubate the specimen in the Round 1 readout buffer at 37° C. for 1 hour.   e. Incubate the specimen in the Wash buffer at 42° C. for 15 minutes.   f. Incubate the specimen in 5×SSC+DAPI at room temperature for 15 minutes.   g. Flush the specimen with 2×SSC for imaging.   h. Perform image acquisition, exciting the specimen with 633 nm, 561 nm, 514 nm, 488 nm, and 405 nm lines and collect spectra.   i. Incubate the specimen in the Round 2 readout buffer at 37° C. for 1 hour.   j. Repeat steps e-h.   k. Incubate the specimen in the Round 3 readout buffer at 37° C. for 1 hour.   l. Repeat steps e-h.       

     Table 8 shows the encoding, readout, and exchange probe sequences used in this example. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Encoding, readout, and exchange probe sequences used in Example 12 
               
            
           
           
               
               
               
            
               
                 SEQ ID 
                   
                   
               
               
                 NO: 
                 Probe Name 
                 Sequence (in 5′ to 3′ order) 
               
               
                   
               
            
           
           
               
               
               
            
               
                 597 
                 Readout Probe 11 
                 /5Alex532/CCCAACTACCCATATTAACACACCC 
               
               
                   
               
               
                 9 
                 Readout Probe 9 
                 /5Alex405N/TTCTCCCTCTATCAACTCTA 
               
               
                   
               
               
                 599 
                 Readout Probe 13 
                 /5Alex647N/CACCCTCATATCTATTACCCTCCCA 
               
               
                   
               
               
                 600 
                 Readout Probe 14 
                 /5Alex488N/TCCCTCCTTACTATTACACTCACCC 
               
               
                   
               
               
                 602 
                 Readout Probe 16 
                 /5Alex546N/CCCTTCTACTACTTCCATACATCCC 
               
               
                   
               
               
                 603 
                 Readout Probe 17 
                 /56-ROXN/CCCTTCTAATCCTATACACTCACCC 
               
               
                   
               
               
                 605 
                 Readout Probe 19 
                 /5Alex532/TCCCTCCTCTTAATACATCCTCCTC 
               
               
                   
               
               
                 606 
                 Readout Probe 20 
                 /56-ROXN/CATCCCTACTTACTTATCCTCCACC 
               
               
                   
               
               
                 607 
                 Readout Probe 21 
                 /5Alex647N/CCCTTCTCCATAACTATACCCTTCC 
               
               
                   
               
               
                 608 
                 Exchange Probe 5 
                 GGGTGTGTTAATATGGGTAGTTGGG 
               
               
                   
               
               
                 610 
                 Exchange Probe 10 
                 TGGGAGGGTAATAGATATGAGGGTG 
               
               
                   
               
               
                 611 
                 Exchange Probe 14 
                 GGGTGAGTGTAATAGTAAGGAGGGA 
               
               
                   
               
               
                 613 
                 Exchange Probe 17 
                 GGGATGTATGGAAGTAGTAGAAGGG 
               
               
                   
               
               
                 614 
                 Exchange Probe 18 
                 GGGTGAGTGTATAGGATTAGAAGGG 
               
               
                   
               
               
                 616 
                 Exchange Probe 25 
                 GAGGAGGATGTATTAAGAGGAGGGA 
               
               
                   
               
               
                 617 
                 Exchange Probe 28 
                 GGTGGAGGATAAGTAAGTAGGGATG 
               
               
                   
               
               
                 618 
                 Exchange Probe 30 
                 GGAAGGGTATAGTTATGGAGAAGGG 
               
               
                   
               
               
                 643 
                 Encoding Probe 605 
                 TTAATATGGGTAGTTGGGTGGATGCCCCTCGACT 
               
               
                   
                   
                 TGCATGACA 
               
               
                   
               
               
                 644 
                 Encoding Probe 606 
                 TTAATATGGGTAGTTGGGACAGGGACCTTCCTCT 
               
               
                   
                   
                 CAGAAAGG 
               
               
                   
               
               
                 645 
                 Encoding Probe 607 
                 TTAATATGGGTAGTTGGGCGTGAGTTAGCCGAT 
               
               
                   
                   
                 GCTTTTAGA 
               
               
                   
               
               
                 646 
                 Encoding Probe 608 
                 TTAATATGGGTAGTTGGGCATCTGCCTTCGCAAT 
               
               
                   
                   
                 CGGAGAAG 
               
               
                   
               
               
                 647 
                 Encoding Probe 609 
                 TTAATATGGGTAGTTGGGTCCCCTCGCGTATCAT 
               
               
                   
                   
                 CGAATATT 
               
               
                   
               
               
                 648 
                 Encoding Probe 610 
                 TTAATATGGGTAGTTGGGCCCTGCGCTCGTTATG 
               
               
                   
                   
                 GCACTATT 
               
               
                   
               
               
                 649 
                 Encoding Probe 611 
                 TTAATATGGGTAGTTGGGTGTACTGATGCGCGAT 
               
               
                   
                   
                 TACTAGGCT 
               
               
                   
               
               
                 650 
                 Encoding Probe 612 
                 TTAATATGGGTAGTTGGGTGGCGGCTTCCATGGC 
               
               
                   
                   
                 TTGACCCC 
               
               
                   
               
               
                 651 
                 Encoding Probe 613 
                 TTAATATGGGTAGTTGGGTGCTTGCATGTGTTAA 
               
               
                   
                   
                 GCCTGTGCG 
               
               
                   
               
               
                 652 
                 Encoding Probe 614 
                 TTAATATGGGTAGTTGGGCCCACCTTCCTCTCAG 
               
               
                   
                   
                 AACCCGAT 
               
               
                   
               
               
                 653 
                 Encoding Probe 615 
                 GATAAGTAAGTAGGGATGGGATGCCCCTCGACT 
               
               
                   
                   
                 TGCATGACA 
               
               
                   
               
               
                 654 
                 Encoding Probe 616 
                 GATAAGTAAGTAGGGATGACAGGGACCTTCCTC 
               
               
                   
                   
                 TCAGAAAGG 
               
               
                   
               
               
                 655 
                 Encoding Probe 617 
                 GATAAGTAAGTAGGGATGCGTGAGTTAGCCGAT 
               
               
                   
                   
                 GCTTTTAGA 
               
               
                   
               
               
                 656 
                 Encoding Probe 618 
                 GATAAGTAAGTAGGGATGCATCTGCCTTCGCAA 
               
               
                   
                   
                 TCGGAGAAG 
               
               
                   
               
               
                 657 
                 Encoding Probe 619 
                 GATAAGTAAGTAGGGATGTCCCCTCGCGTATCA 
               
               
                   
                   
                 TCGAATATT 
               
               
                   
               
               
                 658 
                 Encoding Probe 620 
                 GATAAGTAAGTAGGGATGCCCTGCGCTCGTTAT 
               
               
                   
                   
                 GGCACTATT 
               
               
                   
               
               
                 659 
                 Encoding Probe 621 
                 GATAAGTAAGTAGGGATGGTACTGATGCGCGAT 
               
               
                   
                   
                 TACTAGGCT 
               
               
                   
               
               
                 660 
                 Encoding Probe 622 
                 GATAAGTAAGTAGGGATGTGGCGGCTTCCATGG 
               
               
                   
                   
                 CTTGACCCC 
               
               
                   
               
               
                 661 
                 Encoding Probe 623 
                 GATAAGTAAGTAGGGATGGCTTGCATGTGTTAA 
               
               
                   
                   
                 GCCTGTGCG 
               
               
                   
               
               
                 662 
                 Encoding Probe 624 
                 GATAAGTAAGTAGGGATGCCCACCTTCCTCTCA 
               
               
                   
                   
                 GAACCCGAT 
               
               
                   
               
               
                 663 
                 Encoding Probe 625 
                 ATGGAAGTAGTAGAAGGGTCCCAGGTTGGTCAC 
               
               
                   
                   
                 GTGTTAGAG 
               
               
                   
               
               
                 664 
                 Encoding Probe 626 
                 ATGGAAGTAGTAGAAGGGTGGACCTACTACCTA 
               
               
                   
                   
                 ATGGGCCCGC 
               
               
                   
               
               
                 665 
                 Encoding Probe 627 
                 ATGGAAGTAGTAGAAGGGTGAACTGCTGAAAGC 
               
               
                   
                   
                 GGTTTACTTG 
               
               
                   
               
               
                 666 
                 Encoding Probe 628 
                 ATGGAAGTAGTAGAAGGGAAGGATATCTGCGCA 
               
               
                   
                   
                 TTCCACGCG 
               
               
                   
               
               
                 667 
                 Encoding Probe 629 
                 ATGGAAGTAGTAGAAGGGTAATTTGAGTTTTAG 
               
               
                   
                   
                 CCTTGCCCG 
               
               
                   
               
               
                 668 
                 Encoding Probe 630 
                 ATGGAAGTAGTAGAAGGGTGAATGCTGGCAACA 
               
               
                   
                   
                 CGGGACTCC 
               
               
                   
               
               
                 669 
                 Encoding Probe 631 
                 ATGGAAGTAGTAGAAGGGCCGCGGGTGCAGAC 
               
               
                   
                   
                 GACTCGGCAC 
               
               
                   
               
               
                 670 
                 Encoding Probe 632 
                 ATGGAAGTAGTAGAAGGGTGGAACCCTCCACAC 
               
               
                   
                   
                 CTTCGACCGG 
               
               
                   
               
               
                 671 
                 Encoding Probe 633 
                 ATGGAAGTAGTAGAAGGGCCCAGGTTGGTCACG 
               
               
                   
                   
                 TGTTACAGT 
               
               
                   
               
               
                 672 
                 Encoding Probe 634 
                 ATGGAAGTAGTAGAAGGGTGGACTACTACCTAA 
               
               
                   
                   
                 TGGGCCGGCT 
               
               
                   
               
               
                 673 
                 Encoding Probe 635 
                 TAGAATTAGAGAGATGGGTCCCAGGTTGGTCAC 
               
               
                   
                   
                 GTGTTAGAG 
               
               
                   
               
               
                 674 
                 Encoding Probe 636 
                 TAGAATTAGAGAGATGGGTGGACCTACTACCTA 
               
               
                   
                   
                 ATGGGCCCGC 
               
               
                   
               
               
                 675 
                 Encoding Probe 637 
                 TAGAATTAGAGAGATGGGTGAACTGCTGAAAGC 
               
               
                   
                   
                 GGTTTACTTG 
               
               
                   
               
               
                 676 
                 Encoding Probe 638 
                 TAGAATTAGAGAGATGGGAAGGATATCTGCGCA 
               
               
                   
                   
                 TTCCACGCG 
               
               
                   
               
               
                 677 
                 Encoding Probe 639 
                 TAGAATTAGAGAGATGGGTAATTTGAGTTTTAG 
               
               
                   
                   
                 CCTTGCCCG 
               
               
                   
               
               
                 678 
                 Encoding Probe 640 
                 TAGAATTAGAGAGATGGGTGAATGCTGGCAACA 
               
               
                   
                   
                 CGGGACTCC 
               
               
                   
               
               
                 679 
                 Encoding Probe 641 
                 TAGAATTAGAGAGATGGGCCGCGGGTGCAGACG 
               
               
                   
                   
                 ACTCGGCAC 
               
               
                   
               
               
                 680 
                 Encoding Probe 642 
                 TAGAATTAGAGAGATGGGTGGAACCCTCCACAC 
               
               
                   
                   
                 CTTCGACCGG 
               
               
                   
               
               
                 681 
                 Encoding Probe 643 
                 TAGAATTAGAGAGATGGGCCCAGGTTGGTCACG 
               
               
                   
                   
                 TGTTACAGT 
               
               
                   
               
               
                 682 
                 Encoding Probe 644 
                 TAGAATTAGAGAGATGGGTGGACTACTACCTAA 
               
               
                   
                   
                 TGGGCCGGCT 
               
               
                   
               
               
                 683 
                 Encoding Probe 645 
                 GGAATTTAGTGAGAAGGGTTATTCGATACTATG 
               
               
                   
                   
                 CGGTATTTTA 
               
               
                   
               
               
                 684 
                 Encoding Probe 646 
                 GGAATTTAGTGAGAAGGGCAACCCCATTGTGAA 
               
               
                   
                   
                 TGATTCTGCT 
               
               
                   
               
               
                 685 
                 Encoding Probe 647 
                 GGAATTTAGTGAGAAGGGTGGACCATTACTCTA 
               
               
                   
                   
                 GTCTCGCTCA 
               
               
                   
               
               
                 686 
                 Encoding Probe 648 
                 GGAATTTAGTGAGAAGGGCACCCTCTCGATATC 
               
               
                   
                   
                 TACGCAAAA 
               
               
                   
               
               
                 687 
                 Encoding Probe 649 
                 GGAATTTAGTGAGAAGGGTGCGCCGAAGAGTCG 
               
               
                   
                   
                 CATGCTTAGT 
               
               
                   
               
               
                 688 
                 Encoding Probe 650 
                 GGAATTTAGTGAGAAGGGTGGAGGCATAAGGGC 
               
               
                   
                   
                 CATACTGTGG 
               
               
                   
               
               
                 689 
                 Encoding Probe 651 
                 GGAATTTAGTGAGAAGGGCGCTGGCTTCAGATA 
               
               
                   
                   
                 CTTCGGCAC 
               
               
                   
               
               
                 690 
                 Encoding Probe 652 
                 GGAATTTAGTGAGAAGGGTGGTTACCAGTCTCA 
               
               
                   
                   
                 CCTTAGGTGG 
               
               
                   
               
               
                 691 
                 Encoding Probe 653 
                 GGAATTTAGTGAGAAGGGTTATTCGATACTATG 
               
               
                   
                   
                 CGGTATTATAG 
               
               
                   
               
               
                 692 
                 Encoding Probe 654 
                 GGAATTTAGTGAGAAGGGAAACCCATTGTGAAT 
               
               
                   
                   
                 GATTCTCCTG 
               
               
                   
               
               
                 693 
                 Encoding Probe 655 
                 TGTAATAGTAAGGAGGGATTATTCGATACTATG 
               
               
                   
                   
                 CGGTATTTTA 
               
               
                   
               
               
                 694 
                 Encoding Probe 656 
                 TGTAATAGTAAGGAGGGACAACCCCATTGTGAA 
               
               
                   
                   
                 TGATTCTGCT 
               
               
                   
               
               
                 695 
                 Encoding Probe 657 
                 TGTAATAGTAAGGAGGGAGGACCATTACTCTAG 
               
               
                   
                   
                 TCTCGCTCA 
               
               
                   
               
               
                 696 
                 Encoding Probe 658 
                 TGTAATAGTAAGGAGGGACACCCTCTCGATATC 
               
               
                   
                   
                 TACGCAAAA 
               
               
                   
               
               
                 697 
                 Encoding Probe 659 
                 TGTAATAGTAAGGAGGGAGCGCCGAAGAGTCGC 
               
               
                   
                   
                 ATGCTTAGT 
               
               
                   
               
               
                 698 
                 Encoding Probe 660 
                 TGTAATAGTAAGGAGGGAGGAGGCATAAGGGC 
               
               
                   
                   
                 CATACTGTGG 
               
               
                   
               
               
                 699 
                 Encoding Probe 661 
                 TGTAATAGTAAGGAGGGACGCTGGCTTCAGATA 
               
               
                   
                   
                 CTTCGGCAC 
               
               
                   
               
               
                 700 
                 Encoding Probe 662 
                 TGTAATAGTAAGGAGGGAGGTTACCAGTCTCAC 
               
               
                   
                   
                 CTTAGGTGG 
               
               
                   
               
               
                 701 
                 Encoding Probe 663 
                 TGTAATAGTAAGGAGGGATTATTCGATACTATG 
               
               
                   
                   
                 CGGTATTATAG 
               
               
                   
               
               
                 702 
                 Encoding Probe 664 
                 TGTAATAGTAAGGAGGGAAAACCCATTGTGAAT 
               
               
                   
                   
                 GATTCTCCTG 
               
               
                   
               
               
                 703 
                 Encoding Probe 665 
                 GTAATAGATATGAGGGTGTAGCGCGTTACTCAC 
               
               
                   
                   
                 CCGTCCCGG 
               
               
                   
               
               
                 704 
                 Encoding Probe 666 
                 GTAATAGATATGAGGGTGAACGAAGATTCCCTA 
               
               
                   
                   
                 CTGCTGGGA 
               
               
                   
               
               
                 705 
                 Encoding Probe 667 
                 GTAATAGATATGAGGGTGGCGGCCACCTACGTA 
               
               
                   
                   
                 TTACCGGCC 
               
               
                   
               
               
                 706 
                 Encoding Probe 668 
                 GTAATAGATATGAGGGTGAAGCGCTACACTAGG 
               
               
                   
                   
                 AATTCCCGA 
               
               
                   
               
               
                 707 
                 Encoding Probe 669 
                 GTAATAGATATGAGGGTGGCGTGCTTCGAATTA 
               
               
                   
                   
                 AACCACTAC 
               
               
                   
               
               
                 708 
                 Encoding Probe 670 
                 GTAATAGATATGAGGGTGAACGGACTTAACCCA 
               
               
                   
                   
                 ACATCTGTG 
               
               
                   
               
               
                 709 
                 Encoding Probe 671 
                 GTAATAGATATGAGGGTGGGTCATTGTAGCACG 
               
               
                   
                   
                 TGTGTACGG 
               
               
                   
               
               
                 710 
                 Encoding Probe 672 
                 GTAATAGATATGAGGGTGTGTCTCTCATGGTGTG 
               
               
                   
                   
                 ACGGGACC 
               
               
                   
               
               
                 711 
                 Encoding Probe 673 
                 GTAATAGATATGAGGGTGACGACGCGTTACTCA 
               
               
                   
                   
                 CCCGTCGCG 
               
               
                   
               
               
                 712 
                 Encoding Probe 674 
                 GTAATAGATATGAGGGTGCAACCCTCTCAGGTC 
               
               
                   
                   
                 GGCTACCGT 
               
               
                   
               
               
                 713 
                 Encoding Probe 675 
                 ATGTATTAAGAGGAGGGATAGCGCGTTACTCAC 
               
               
                   
                   
                 CCGTCCCGG 
               
               
                   
               
               
                 714 
                 Encoding Probe 676 
                 ATGTATTAAGAGGAGGGAAACGAAGATTCCCTA 
               
               
                   
                   
                 CTGCTGGGA 
               
               
                   
               
               
                 715 
                 Encoding Probe 677 
                 ATGTATTAAGAGGAGGGAGCGGCCACCTACGTA 
               
               
                   
                   
                 TTACCGGCC 
               
               
                   
               
               
                 716 
                 Encoding Probe 678 
                 ATGTATTAAGAGGAGGGAAAGCGCTACACTAGG 
               
               
                   
                   
                 AATTCCCGA 
               
               
                   
               
               
                 717 
                 Encoding Probe 679 
                 ATGTATTAAGAGGAGGGAGCGTGCTTCGAATTA 
               
               
                   
                   
                 AACCACTAC 
               
               
                   
               
               
                 718 
                 Encoding Probe 680 
                 ATGTATTAAGAGGAGGGAAACGGACTTAACCCA 
               
               
                   
                   
                 ACATCTGTG 
               
               
                   
               
               
                 719 
                 Encoding Probe 681 
                 ATGTATTAAGAGGAGGGAGGTCATTGTAGCACG 
               
               
                   
                   
                 TGTGTACGG 
               
               
                   
               
               
                 720 
                 Encoding Probe 682 
                 ATGTATTAAGAGGAGGGATGTCTCTCATGGTGT 
               
               
                   
                   
                 GACGGGACC 
               
               
                   
               
               
                 721 
                 Encoding Probe 683 
                 ATGTATTAAGAGGAGGGAACGACGCGTTACTCA 
               
               
                   
                   
                 CCCGTCGCG 
               
               
                   
               
               
                 722 
                 Encoding Probe 684 
                 ATGTATTAAGAGGAGGGACAACCCTCTCAGGTC 
               
               
                   
                   
                 GGCTACCGT 
               
               
                   
               
               
                 723 
                 Encoding Probe 685 
                 GGTAATTGAGTAGAAGGGTGTCCCCTCCTTAAG 
               
               
                   
                   
                 CAGATCTGAG 
               
               
                   
               
               
                 724 
                 Encoding Probe 686 
                 GGTAATTGAGTAGAAGGGTGTACGAATAACTTC 
               
               
                   
                   
                 TTCGTTCAGCG 
               
               
                   
               
               
                 725 
                 Encoding Probe 687 
                 GGTAATTGAGTAGAAGGGTCTTACTATGCCATCT 
               
               
                   
                   
                 ACGCAAGG 
               
               
                   
               
               
                 726 
                 Encoding Probe 688 
                 GGTAATTGAGTAGAAGGGTGACCTCTGTCATAC 
               
               
                   
                   
                 TCTAGCTAAC 
               
               
                   
               
               
                 727 
                 Encoding Probe 689 
                 GGTAATTGAGTAGAAGGGTGTGCCTGTATGCAC 
               
               
                   
                   
                 GCTATCCAGG 
               
               
                   
               
               
                 728 
                 Encoding Probe 690 
                 GGTAATTGAGTAGAAGGGCGAGGTCATATAGGG 
               
               
                   
                   
                 CATGATGTAA 
               
               
                   
               
               
                 729 
                 Encoding Probe 691 
                 GGTAATTGAGTAGAAGGGTGCTCCATCTTCATG 
               
               
                   
                   
                 AAGTCGACAA 
               
               
                   
               
               
                 730 
                 Encoding Probe 692 
                 GGTAATTGAGTAGAAGGGTGCTCCCTCTTTCGTT 
               
               
                   
                   
                 AGGCCTGG 
               
               
                   
               
               
                 731 
                 Encoding Probe 693 
                 GGTAATTGAGTAGAAGGGCGACCTCGTCTTAAG 
               
               
                   
                   
                 GGTAGGAAT 
               
               
                   
               
               
                 732 
                 Encoding Probe 694 
                 GGTAATTGAGTAGAAGGGTGTACGAATAACTTC 
               
               
                   
                   
                 TTCGTTCTGC 
               
               
                   
               
               
                 733 
                 Encoding Probe 695 
                 TTGTAAAATAGGGAGGGAGTCCCCTCCTTAAGC 
               
               
                   
                   
                 AGATCTGAG 
               
               
                   
               
               
                 734 
                 Encoding Probe 696 
                 TTGTAAAATAGGGAGGGAGTACGAATAACTTCT 
               
               
                   
                   
                 TCGTTCAGCG 
               
               
                   
               
               
                 735 
                 Encoding Probe 697 
                 TTGTAAAATAGGGAGGGATCTTACTATGCCATCT 
               
               
                   
                   
                 ACGCAAGG 
               
               
                   
               
               
                 736 
                 Encoding Probe 698 
                 TTGTAAAATAGGGAGGGATGACCTCTGTCATAC 
               
               
                   
                   
                 TCTAGCTAAC 
               
               
                   
               
               
                 737 
                 Encoding Probe 699 
                 TTGTAAAATAGGGAGGGAGTGCCTGTATGCACG 
               
               
                   
                   
                 CTATCCAGG 
               
               
                   
               
               
                 738 
                 Encoding Probe 700 
                 TTGTAAAATAGGGAGGGACGAGGTCATATAGGG 
               
               
                   
                   
                 CATGATGTAA 
               
               
                   
               
               
                 739 
                 Encoding Probe 701 
                 TTGTAAAATAGGGAGGGAGCTCCATCTTCATGA 
               
               
                   
                   
                 AGTCGACAA 
               
               
                   
               
               
                 740 
                 Encoding Probe 702 
                 TTGTAAAATAGGGAGGGATGCTCCCTCTTTCGTT 
               
               
                   
                   
                 AGGCCTGG 
               
               
                   
               
               
                 741 
                 Encoding Probe 703 
                 TTGTAAAATAGGGAGGGACGACCTCGTCTTAAG 
               
               
                   
                   
                 GGTAGGAAT 
               
               
                   
               
               
                 742 
                 Encoding Probe 704 
                 TTGTAAAATAGGGAGGGATGTACGAATAACTTC 
               
               
                   
                   
                 TTCGTTCTGC 
               
               
                   
               
               
                 743 
                 Encoding Probe 705 
                 TGTATAGGATTAGAAGGGACACCCACGAGCGGA 
               
               
                   
                   
                 CACGTTGGC 
               
               
                   
               
               
                 744 
                 Encoding Probe 706 
                 TGTATAGGATTAGAAGGGTGTGGGAATAGCTGG 
               
               
                   
                   
                 ATCAGGCAAC 
               
               
                   
               
               
                 745 
                 Encoding Probe 707 
                 TGTATAGGATTAGAAGGGCAACCGGTGCTTATT 
               
               
                   
                   
                 CTTAGAGATG 
               
               
                   
               
               
                 746 
                 Encoding Probe 708 
                 TGTATAGGATTAGAAGGGTGTGCCCTCTGACAC 
               
               
                   
                   
                 ACTCTAGAGC 
               
               
                   
               
               
                 747 
                 Encoding Probe 709 
                 TGTATAGGATTAGAAGGGAATAGAGCTTCCTGA 
               
               
                   
                   
                 CATGTCTTC 
               
               
                   
               
               
                 748 
                 Encoding Probe 710 
                 TGTATAGGATTAGAAGGGTGGATCGTAGCAACT 
               
               
                   
                   
                 AGTGACATCC 
               
               
                   
               
               
                 749 
                 Encoding Probe 711 
                 TGTATAGGATTAGAAGGGTGACATCCGGACTAC 
               
               
                   
                   
                 GATCGGTAAA 
               
               
                   
               
               
                 750 
                 Encoding Probe 712 
                 TGTATAGGATTAGAAGGGCCAGGCTAACGACTT 
               
               
                   
                   
                 CTGGTATTG 
               
               
                   
               
               
                 751 
                 Encoding Probe 713 
                 TGTATAGGATTAGAAGGGAACCCCCACGAGCGG 
               
               
                   
                   
                 ACACGTAGG 
               
               
                   
               
               
                 752 
                 Encoding Probe 714 
                 TGTATAGGATTAGAAGGGTGCGAATAGCTGGAT 
               
               
                   
                   
                 CAGGCTTCGC 
               
               
                   
               
               
                 753 
                 Encoding Probe 715 
                 TATAGTTATGGAGAAGGGACACCCACGAGCGGA 
               
               
                   
                   
                 CACGTTGGC 
               
               
                   
               
               
                 754 
                 Encoding Probe 716 
                 TATAGTTATGGAGAAGGGTGTGGGAATAGCTGG 
               
               
                   
                   
                 ATCAGGCAAC 
               
               
                   
               
               
                 755 
                 Encoding Probe 717 
                 TATAGTTATGGAGAAGGGCAACCGGTGCTTATT 
               
               
                   
                   
                 CTTAGAGATG 
               
               
                   
               
               
                 756 
                 Encoding Probe 718 
                 TATAGTTATGGAGAAGGGTGTGCCCTCTGACAC 
               
               
                   
                   
                 ACTCTAGAGC 
               
               
                   
               
               
                 757 
                 Encoding Probe 719 
                 TATAGTTATGGAGAAGGGAATAGAGCTTCCTGA 
               
               
                   
                   
                 CATGTCTTC 
               
               
                   
               
               
                 758 
                 Encoding Probe 720 
                 TATAGTTATGGAGAAGGGTGGATCGTAGCAACT 
               
               
                   
                   
                 AGTGACATCC 
               
               
                   
               
               
                 759 
                 Encoding Probe 721 
                 TATAGTTATGGAGAAGGGTGACATCCGGACTAC 
               
               
                   
                   
                 GATCGGTAAA 
               
               
                   
               
               
                 760 
                 Encoding Probe 722 
                 TATAGTTATGGAGAAGGGCCAGGCTAACGACTT 
               
               
                   
                   
                 CTGGTATTG 
               
               
                   
               
               
                 761 
                 Encoding Probe 723 
                 TATAGTTATGGAGAAGGGAACCCCCACGAGCGG 
               
               
                   
                   
                 ACACGTAGG 
               
               
                   
               
               
                 762 
                 Encoding Probe 724 
                 TATAGTTATGGAGAAGGGTGCGAATAGCTGGAT 
               
               
                   
                   
                 CAGGCTTCGC 
               
               
                   
               
            
           
         
       
     
     Example 13. Species-Level Swap in Tissue Samples 
     To extend the ability to perform HiPR-Swap at the phylum level on a tissue specimen (colon of a healthy mouse) to the species level probes to perform a simple taxon identification experiment were designed, barcoding the sixty-five most abundant species in healthy mouse stool (measured internally by PacBio 16S long-read sequencing). As shown in  FIG.  19   , we were able to identified several dozen species. Signal exchange correctly matched expectations for taxa present in the encoding panel. 
     Species-level swap protocol: OCT-embedded formalin-fixed tissue was sectioned at 10-micron thickness onto circular glass coverslips made for Bioptechs FCS2 flow cell. The tissue was covered with 2% formaldehyde for two hours at room temperature to fix the sample. The sample was washed by removing the buffer and replacing it with 1×PBS for 5 minutes (this was repeated two more times). The fixed tissue specimen was stored in 70% ethanol at 4° C. overnight. The following buffers were prepared:
         Encoding buffer: Encoding probes (100 nM of each encoding probe in complex pool, 5 nM of blocking probes in the complex probe pool+EUB at 1 μM); 2× sodium chloride sodium citrate (SSC); 5×Denhardt&#39;s solution; 10% dextran sulfate; 10% ethylene carbonate; and 0.01% sodium dodecyl sulfate (SDS)   Round 1 readout buffer: 400 nM each of readout probes: 21, 11, 22, 12, 13, and 9; 2× sodium chloride sodium citrate (SSC); 5×Denhardt&#39;s solution; 10% dextran sulfate; 10% ethylene carbonate; and 0.01% sodium dodecyl sulfate (SDS)   Round 2 readout buffer: 10 μM each of exchange probe: 4-5, 7-8, and 10; 400 nM each of readout probes: 14, 24, 16, 17, and 23; 2× sodium chloride sodium citrate (SSC); 5×Denhardt&#39;s solution; 10% dextran sulfate; 10% ethylene carbonate; and 0.01% sodium dodecyl sulfate (SDS)   Round 3 readout buffer: 10 μM each of exchange probe: 14, 21, 17-18, and 20; 400 nM each of readout probes: 18, 19, 25, 20, and 21; 2× sodium chloride sodium citrate (SSC); 5×Denhardt&#39;s solution; 10% dextran sulfate; 10% ethylene carbonate; 0.01% sodium dodecyl sulfate (SDS)   Wash buffer: 215 mM NaCl; 20 mM Tris-HCl (pH 7.5); and 5 mM EDTA       

     Place 10 mg/mL lysozyme to completely cover the specimen and incubate for 30 minutes at 37° C. in a humidified chamber. Wash the specimen with 1×PBS for 15 minutes at room temperature. Dry the specimen by submerging it in 100% ethanol and allowing it to air dry. Place the coverslip on the FCS2 flow cell (Bioptechs) and assemble. Place the flow cell assembly on the microscope stage (Zeiss i880 confocal). Connect the flow cell input port to the Aria Automated Perfusion System (Fluigent). Calibrate the Aria Automated Perfusion System using DI water. Load encoding, readout buffers, wash buffer, 1×PBS buffer, 5×SSC+DAPI buffer (40 ng/mL DAPI in 5×SSC), and 2×SSC buffer into Aria Automated Perfusion System at the desired reservoir locations. Execute the following sequence on the Aria:
         a. Incubate the specimen with 1×PBS at room temperature for 15 minutes.   b. Incubate the specimen in the Encoding buffer at 37° C. for 2 hours.   c. Incubate the specimen in the Wash buffer at 42° C. for 15 minutes.   d. Incubate the specimen in the Round 1 readout buffer at 37° C. for 1 hour.   e. Incubate the specimen in the Wash buffer at 42° C. for 15 minutes.   f. Incubate the specimen in 5×SSC+DAPI at room temperature for 15 minutes.   g. Flush the specimen with 2×SSC for imaging.   h. Perform image acquisition, exciting the specimen with 633 nm, 561 nm, 514 nm, 488 nm, and 405 nm lines and collect spectra.   i. Incubate the specimen in the Round 2 readout buffer at 37° C. for 1 hour.   j. Repeat steps e-h.   k. Incubate the specimen in the Round 3 readout buffer at 37° C. for 1 hour.   l. Repeat steps e-h.       

     Table 9 shows the encoding, readout, and exchange probe sequences used in this example. 
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Encoding, readout, and exchange probe sequences used in Example 13 
               
            
           
           
               
               
               
            
               
                 SEQ ID 
                   
                   
               
               
                 NO: 
                 Probe Name 
                 Sequence (in 5′ to 3′ order) 
               
               
                   
               
            
           
           
               
               
               
            
               
                 763 
                 Readout Probe 
                 /5Alex488N/CCCTTCTACTCAATTACCTCATCCC 
               
               
                   
                 21 
                   
               
               
                   
               
               
                 597 
                 Readout Probe 
                 /5Alex532/CCCAACTACCCATATTAACACACCC 
               
               
                   
                 11 
                   
               
               
                   
               
               
                 764 
                 Readout Probe 
                 /5Alex546N/CCCATCTCACTATCTTATCACCCAC 
               
               
                   
                 22 
                   
               
               
                   
               
               
                 598 
                 Readout Probe 
                 /56-ROXN/CCCTTCTCACTAAATTCCAACACCC 
               
               
                   
                 12 
                   
               
               
                   
               
               
                 9 
                 Readout Probe 
                 /5Alex405N/TTCTCCCTCTATCAACTCTA 
               
               
                   
                 9 
                   
               
               
                   
               
               
                 599 
                 Readout Probe 
                 /5Alex647N/CACCCTCATATCTATTACCCTCCCA 
               
               
                   
                 13 
                   
               
               
                   
               
               
                 600 
                 Readout Probe 
                 /5Alex488N/TCCCTCCTTACTATTACACTCACCC 
               
               
                   
                 14 
                   
               
               
                   
               
               
                 602 
                 Readout Probe 
                 /5Alex546N/CCCTTCTACTACTTCCATACATCCC 
               
               
                   
                 16 
                   
               
               
                   
               
               
                 603 
                 Readout Probe 
                 /56-ROXN/CCCTTCTAATCCTATACACTCACCC 
               
               
                   
                 17 
                   
               
               
                   
               
               
                 765 
                 Readout Probe 
                 /5Alex647N/CCCACTCAACATATCATTCCACCAC 
               
               
                   
                 23 
                   
               
               
                   
               
               
                 766 
                 Readout Probe 
                 /5Alex532/CCCAACTATACTCTATCCTCCATCC 
               
               
                   
                 24 
                   
               
               
                   
               
               
                 604 
                 Readout Probe 
                 /5Alex488N/CCCATCTCTCTAATTCTACTCCACC 
               
               
                   
                 18 
                   
               
               
                   
               
               
                 605 
                 Readout Probe 
                 /5Alex532/TCCCTCCTCTTAATACATCCTCCTC 
               
               
                   
                 19 
                   
               
               
                   
               
               
                 767 
                 Readout Probe 
                 /5Alex546N/CCTCACCCTAATAATACTCCAACCC 
               
               
                   
                 25 
                   
               
               
                   
               
               
                 606 
                 Readout Probe 
                 /56-ROXN/CATCCCTACTTACTTATCCTCCACC 
               
               
                   
                 20 
                   
               
               
                   
               
               
                 607 
                 Readout Probe 
                 /5Alex647N/CCCTTCTCCATAACTATACCCTTCC 
               
               
                   
                 21 
                   
               
               
                   
               
               
                 768 
                 Exchange 
                 GGGATGAGGTAATTGAGTAGAAGGG 
               
               
                   
                 Probe 4 
                   
               
               
                   
               
               
                 608 
                 Exchange 
                 GGGTGTGTTAATATGGGTAGTTGGG 
               
               
                   
                 Probe 5 
                   
               
               
                   
               
               
                 769 
                 Exchange 
                 GTGGGTGATAAGATAGTGAGATGGG 
               
               
                   
                 Probe 7 
                   
               
               
                   
               
               
                 609 
                 Exchange 
                 GGGTGTTGGAATTTAGTGAGAAGGG 
               
               
                   
                 Probe 8 
                   
               
               
                   
               
               
                 610 
                 Exchange 
                 TGGGAGGGTAATAGATATGAGGGTG 
               
               
                   
                 Probe 10 
                   
               
               
                   
               
               
                 611 
                 Exchange 
                 GGGTGAGTGTAATAGTAAGGAGGGA 
               
               
                   
                 Probe 14 
                   
               
               
                   
               
               
                 613 
                 Exchange 
                 GGGATGTATGGAAGTAGTAGAAGGG 
               
               
                   
                 Probe 17 
                   
               
               
                   
               
               
                 614 
                 Exchange 
                 GGGTGAGTGTATAGGATTAGAAGGG 
               
               
                   
                 Probe 18 
                   
               
               
                   
               
               
                 770 
                 Exchange 
                 GTGGTGGAATGATATGTTGAGTGGG 
               
               
                   
                 Probe 20 
                   
               
               
                   
               
               
                 771 
                 Exchange 
                 GGATGGAGGATAGAGTATAGTTGGG 
               
               
                   
                 Probe 21 
                   
               
               
                   
               
               
                 772 
                 Encoding 
                 GTAATAGATATGAGGGTGCGGAGCGTCAGTAGGGC 
               
               
                   
                 Probe 725 
                 GCCGCATTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 773 
                 Encoding 
                 GTAATAGATATGAGGGTGCGCACGCGGTATTAGACG 
               
               
                   
                 Probe 726 
                 GAATTTGAATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 774 
                 Encoding 
                 GTAATAGATATGAGGGTGGACCCCCCGCTGCCCCTC 
               
               
                   
                 Probe 727 
                 GACAACTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 775 
                 Encoding 
                 GTAATAGATATGAGGGTGCGCACGCGGTATTAGACG 
               
               
                   
                 Probe 728 
                 GAATTAGATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 776 
                 Encoding 
                 GTAATAGATATGAGGGTGAATCCGCCGACTAGCTAA 
               
               
                   
                 Probe 729 
                 TGCCGGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 777 
                 Encoding 
                 GTAATAGATATGAGGGTGCGTCTTGCTCCCCGGCAA 
               
               
                   
                 Probe 730 
                 AAGTCCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 778 
                 Encoding 
                 GTAATAGATATGAGGGTGGACCCCCCGCTGCCCCTC 
               
               
                   
                 Probe 731 
                 GACTACGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 779 
                 Encoding 
                 GTAATAGATATGAGGGTGATGCGCCGACTAGCTAAT 
               
               
                   
                 Probe 732 
                 GCGGGCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 780 
                 Encoding 
                 ATGTATTAAGAGGAGGGACGGAGCGTCAGTAGGGC 
               
               
                   
                 Probe 733 
                 GCCGCATGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 781 
                 Encoding 
                 ATGTATTAAGAGGAGGGACGCACGCGGTATTAGACG 
               
               
                   
                 Probe 734 
                 GAATTTGAAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 782 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGACCCCCCGCTGCCCCTC 
               
               
                   
                 Probe 735 
                 GACAACGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 783 
                 Encoding 
                 ATGTATTAAGAGGAGGGACGCACGCGGTATTAGACG 
               
               
                   
                 Probe 736 
                 GAATTAGAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 784 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAATCCGCCGACTAGCTAA 
               
               
                   
                 Probe 737 
                 TGCCGGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 785 
                 Encoding 
                 ATGTATTAAGAGGAGGGACGTCTTGCTCCCCGGCAA 
               
               
                   
                 Probe 738 
                 AAGTCCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 786 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGACCCCCCGCTGCCCCTC 
               
               
                   
                 Probe 739 
                 GACTACGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 787 
                 Encoding 
                 ATGTATTAAGAGGAGGGAATGCGCCGACTAGCTAAT 
               
               
                   
                 Probe 740 
                 GCGGGCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 788 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGGCGTTAAGCCCCGGCAT 
               
               
                   
                 Probe 741 
                 TTCTGAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 789 
                 Encoding 
                 TGTAATAGTAAGGAGGGACCACCCAACACCTAGTAA 
               
               
                   
                 Probe 742 
                 TCATGCAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 790 
                 Encoding 
                 TGTAATAGTAAGGAGGGACTTGAAAGTGACTTTGCT 
               
               
                   
                 Probe 743 
                 CACAGCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 791 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGCAGTAGCCCTGATCATA 
               
               
                   
                 Probe 744 
                 AGGCCGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 792 
                 Encoding 
                 TGTAATAGTAAGGAGGGACCTCATCGTATACCACCA 
               
               
                   
                 Probe 745 
                 GAGTAAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 793 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAGATGCACTCTAGCTGCA 
               
               
                   
                 Probe 746 
                 CAGAAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 794 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGAGCTGCACTCTAGCTGC 
               
               
                   
                 Probe 747 
                 ACACAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 795 
                 Encoding 
                 TGTAATAGTAAGGAGGGACCTCATCGTATACCACCA 
               
               
                   
                 Probe 748 
                 GAGAAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 796 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGGCGTTAAGCCCCGGCA 
               
               
                   
                 Probe 749 
                 TTTCTGAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 797 
                 Encoding 
                 TATAGTTATGGAGAAGGGCCACCCAACACCTAGTAA 
               
               
                   
                 Probe 750 
                 TCATGCAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 798 
                 Encoding 
                 TATAGTTATGGAGAAGGGCTTGAAAGTGACTTTGCT 
               
               
                   
                 Probe 751 
                 CACAGCGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 799 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGCAGTAGCCCTGATCAT 
               
               
                   
                 Probe 752 
                 AAGGCCGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 800 
                 Encoding 
                 TATAGTTATGGAGAAGGGCCTCATCGTATACCACCA 
               
               
                   
                 Probe 753 
                 GAGTAAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 801 
                 Encoding 
                 TATAGTTATGGAGAAGGGAGATGCACTCTAGCTGCA 
               
               
                   
                 Probe 754 
                 CAGAAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 802 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGAGCTGCACTCTAGCTG 
               
               
                   
                 Probe 755 
                 CACACAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 803 
                 Encoding 
                 TATAGTTATGGAGAAGGGCCTCATCGTATACCACCA 
               
               
                   
                 Probe 756 
                 GAGAAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 804 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGAGAAAGGCAGGTTCC 
               
               
                   
                 Probe 757 
                 TCACCGCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 805 
                 Encoding 
                 TTAATATGGGTAGTTGGGTTCGTACCGTCTTCTGCTC 
               
               
                   
                 Probe 758 
                 TTAGGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 806 
                 Encoding 
                 TTAATATGGGTAGTTGGGTCCCCGTCTTCTGCTCTTC 
               
               
                   
                 Probe 759 
                 CCGGAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 807 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGAGAAAGGCAGGTTCC 
               
               
                   
                 Probe 760 
                 TCACGGCAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 808 
                 Encoding 
                 TTAATATGGGTAGTTGGGATTCACATAATCCACCGC 
               
               
                   
                 Probe 761 
                 TTGACGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 809 
                 Encoding 
                 TTAATATGGGTAGTTGGGCCCTCAGTCCCCGCACAC 
               
               
                   
                 Probe 762 
                 CTACATGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 810 
                 Encoding 
                 TTAATATGGGTAGTTGGGTCTAACAGTTTCAAATGC 
               
               
                   
                 Probe 763 
                 AGTTGTGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 811 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGAGGATTTCACATCTG 
               
               
                   
                 Probe 764 
                 ACTTGTAAGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 812 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGAGAAAGGCAGGTTCC 
               
               
                   
                 Probe 765 
                 TCACCGCGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 813 
                 Encoding 
                 AATGATATGTTGAGTGGGTTCGTACCGTCTTCTGCTC 
               
               
                   
                 Probe 766 
                 TTAGGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 814 
                 Encoding 
                 AATGATATGTTGAGTGGGTCCCCGTCTTCTGCTCTTC 
               
               
                   
                 Probe 767 
                 CCGGAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 815 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGAGAAAGGCAGGTTCC 
               
               
                   
                 Probe 768 
                 TCACGGCAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 816 
                 Encoding 
                 AATGATATGTTGAGTGGGATTCACATAATCCACCGC 
               
               
                   
                 Probe 769 
                 TTGACGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 817 
                 Encoding 
                 AATGATATGTTGAGTGGGCCCTCAGTCCCCGCACAC 
               
               
                   
                 Probe 770 
                 CTACATGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 818 
                 Encoding 
                 AATGATATGTTGAGTGGGTCTAACAGTTTCAAATGC 
               
               
                   
                 Probe 771 
                 AGTTGTGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 819 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGAGGATTTCACATCTG 
               
               
                   
                 Probe 772 
                 ACTTGTAAGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 820 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGAGAGAACCCCTAGACA 
               
               
                   
                 Probe 773 
                 TCGTGCGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 821 
                 Encoding 
                 TGTATAGGATTAGAAGGGACGCAGCGTCAGTTGGGC 
               
               
                   
                 Probe 774 
                 GCCGCATGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 822 
                 Encoding 
                 TGTATAGGATTAGAAGGGATGAACGCTTTCGCTGTG 
               
               
                   
                 Probe 775 
                 CCAAGGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 823 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGGCAGTCTCGACAGAGT 
               
               
                   
                 Probe 776 
                 CCTCTCGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 824 
                 Encoding 
                 TGTATAGGATTAGAAGGGACGGTGTTAGGCCTGTCG 
               
               
                   
                 Probe 777 
                 CTACGCGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 825 
                 Encoding 
                 TGTATAGGATTAGAAGGGCGTTGTTAGGCCTGTCGC 
               
               
                   
                 Probe 778 
                 TAGGCAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 826 
                 Encoding 
                 TGTATAGGATTAGAAGGGCGGCTAGCTAATGTCACG 
               
               
                   
                 Probe 779 
                 CATCGGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 827 
                 Encoding 
                 TGTATAGGATTAGAAGGGATGCTCGCCCACTCAAGA 
               
               
                   
                 Probe 780 
                 CCGAGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 828 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGAGAGAACCCCTAGAC 
               
               
                   
                 Probe 781 
                 ATCGTGCGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 829 
                 Encoding 
                 TAGAATTAGAGAGATGGGACGCAGCGTCAGTTGGGC 
               
               
                   
                 Probe 782 
                 GCCGCATGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 830 
                 Encoding 
                 TAGAATTAGAGAGATGGGATGAACGCTTTCGCTGTG 
               
               
                   
                 Probe 783 
                 CCAAGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 831 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGCAGTCTCGACAGAGT 
               
               
                   
                 Probe 784 
                 CCTCTCGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 832 
                 Encoding 
                 TAGAATTAGAGAGATGGGACGGTGTTAGGCCTGTCG 
               
               
                   
                 Probe 785 
                 CTACGCGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 833 
                 Encoding 
                 TAGAATTAGAGAGATGGGCGTTGTTAGGCCTGTCGC 
               
               
                   
                 Probe 786 
                 TAGGCAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 834 
                 Encoding 
                 TAGAATTAGAGAGATGGGCGGCTAGCTAATGTCACG 
               
               
                   
                 Probe 787 
                 CATCGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 835 
                 Encoding 
                 TAGAATTAGAGAGATGGGATGCTCGCCCACTCAAGA 
               
               
                   
                 Probe 788 
                 CCGAGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 836 
                 Encoding 
                 GGAATTTAGTGAGAAGGGACGTGAAACTATACCATC 
               
               
                   
                 Probe 789 
                 GGGTTAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 837 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTAGGCGGTGAAACTATAC 
               
               
                   
                 Probe 790 
                 CATGCCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 838 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGTAAAAATGGTATGCAT 
               
               
                   
                 Probe 791 
                 ACCAAAGAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 839 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTTTGGTATGCATACCAAA 
               
               
                   
                 Probe 792 
                 CTTTAAAGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 840 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTACGTGAAACTATACCAT 
               
               
                   
                 Probe 793 
                 CGGGATAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 841 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGTATGGTATGCATACCA 
               
               
                   
                 Probe 794 
                 AACTAATGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 842 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCGCGAAACTATACCATCG 
               
               
                   
                 Probe 795 
                 GGTAAATGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 843 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCATTACAAAATGGTATGC 
               
               
                   
                 Probe 796 
                 ATACCTTTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 844 
                 Encoding 
                 GGATAGAGTATAGTTGGGACGTGAAACTATACCATC 
               
               
                   
                 Probe 797 
                 GGGTTAAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 845 
                 Encoding 
                 GGATAGAGTATAGTTGGGTAGGCGGTGAAACTATAC 
               
               
                   
                 Probe 798 
                 CATGCCGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 846 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGTAAAAATGGTATGCAT 
               
               
                   
                 Probe 799 
                 ACCAAAGAAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 847 
                 Encoding 
                 GGATAGAGTATAGTTGGGTTTGGTATGCATACCAAA 
               
               
                   
                 Probe 800 
                 CTTTAAAGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 848 
                 Encoding 
                 GGATAGAGTATAGTTGGGTACGTGAAACTATACCAT 
               
               
                   
                 Probe 801 
                 CGGGATAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 849 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGTATGGTATGCATACCA 
               
               
                   
                 Probe 802 
                 AACTAATGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 850 
                 Encoding 
                 GGATAGAGTATAGTTGGGCGCGAAACTATACCATCG 
               
               
                   
                 Probe 803 
                 GGTAAATGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 851 
                 Encoding 
                 GGATAGAGTATAGTTGGGCATTACAAAATGGTATGC 
               
               
                   
                 Probe 804 
                 ATACCTTTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 852 
                 Encoding 
                 GGTAATTGAGTAGAAGGGAATGGGTATTAGTACCAA 
               
               
                   
                 Probe 805 
                 TTTCTCTCAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 853 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCGTCCTTCGCAGGGTAGC 
               
               
                   
                 Probe 806 
                 TGCGGAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 854 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCGGGAAGGGAAACGCTC 
               
               
                   
                 Probe 807 
                 TTTCTTCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 855 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGCGACCGCAACTATTCT 
               
               
                   
                 Probe 808 
                 CTAGAGGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 856 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGGAACATTTCACCTCTA 
               
               
                   
                 Probe 809 
                 ACTTATCATTGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 857 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCGGTCCTTATTCGTACGA 
               
               
                   
                 Probe 810 
                 TACTTAGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 858 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGTCCCCCTATGTATCGT 
               
               
                   
                 Probe 811 
                 CGCCAACGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 859 
                 Encoding 
                 GGTAATTGAGTAGAAGGGAATGGGTATTAGTACCAA 
               
               
                   
                 Probe 812 
                 TTTCTCACACGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 860 
                 Encoding 
                 AGTATTATTAGGGTGAGGAATGGGTATTAGTACCAA 
               
               
                   
                 Probe 813 
                 TTTCTCTCAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 861 
                 Encoding 
                 AGTATTATTAGGGTGAGGCGTCCTTCGCAGGGTAGC 
               
               
                   
                 Probe 814 
                 TGCGGAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 862 
                 Encoding 
                 AGTATTATTAGGGTGAGGCGGGAAGGGAAACGCTCT 
               
               
                   
                 Probe 815 
                 TTCTTCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 863 
                 Encoding 
                 AGTATTATTAGGGTGAGGGCGACCGCAACTATTCTC 
               
               
                   
                 Probe 816 
                 TAGAGGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 864 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGGAACATTTCACCTCTA 
               
               
                   
                 Probe 817 
                 ACTTATCATTGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 865 
                 Encoding 
                 AGTATTATTAGGGTGAGGCGGTCCTTATTCGTACGA 
               
               
                   
                 Probe 818 
                 TACTTAGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 866 
                 Encoding 
                 AGTATTATTAGGGTGAGGGTCCCCCTATGTATCGTC 
               
               
                   
                 Probe 819 
                 GCCAACGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 867 
                 Encoding 
                 AGTATTATTAGGGTGAGGAATGGGTATTAGTACCAA 
               
               
                   
                 Probe 820 
                 TTTCTCACACGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 868 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGGCACTCGAATGCCAC 
               
               
                   
                 Probe 821 
                 ATGATTACTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 869 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGACCTCAAGTTACACAG 
               
               
                   
                 Probe 822 
                 TTTCCTCTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 870 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGGCACTCGAATGCCAC 
               
               
                   
                 Probe 823 
                 ATGATAACGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 871 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGACCGCCACTCGAATG 
               
               
                   
                 Probe 824 
                 CCACAACTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 872 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGAGCTGCACTCAAGTTA 
               
               
                   
                 Probe 825 
                 CACACAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 873 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGGCACTCGAATGCCAC 
               
               
                   
                 Probe 826 
                 ATGAAAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 874 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGACCGCCACTCGAATG 
               
               
                   
                 Probe 827 
                 CCACTACGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 875 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGCCGCCACTCGAATGC 
               
               
                   
                 Probe 828 
                 CACAACTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 876 
                 Encoding 
                 AATGATATGTTGAGTGGGAGGCACTCGAATGCCACA 
               
               
                   
                 Probe 829 
                 TGATTACTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 877 
                 Encoding 
                 AATGATATGTTGAGTGGGTGACCTCAAGTTACACAG 
               
               
                   
                 Probe 830 
                 TTTCCTCTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 878 
                 Encoding 
                 AATGATATGTTGAGTGGGAGGCACTCGAATGCCACA 
               
               
                   
                 Probe 831 
                 TGATAACGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 879 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGACCGCCACTCGAATG 
               
               
                   
                 Probe 832 
                 CCACAACTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 880 
                 Encoding 
                 AATGATATGTTGAGTGGGTGAGCTGCACTCAAGTTA 
               
               
                   
                 Probe 833 
                 CACACAAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 881 
                 Encoding 
                 AATGATATGTTGAGTGGGAGGCACTCGAATGCCACA 
               
               
                   
                 Probe 834 
                 TGAAAAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 882 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGACCGCCACTCGAATG 
               
               
                   
                 Probe 835 
                 CCACTACGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 883 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGCCGCCACTCGAATGC 
               
               
                   
                 Probe 836 
                 CACAACTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 884 
                 Encoding 
                 ATAAGATAGTGAGATGGGAGAGACACTCTAGCAAA 
               
               
                   
                 Probe 837 
                 ACAGTAAGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 885 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGGAGTTTTTCACACACT 
               
               
                   
                 Probe 838 
                 GCCTACGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 886 
                 Encoding 
                 ATAAGATAGTGAGATGGGCCGCGTTACCGGCCCGCC 
               
               
                   
                 Probe 839 
                 AGGGCCTGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 887 
                 Encoding 
                 ATAAGATAGTGAGATGGGTAGAAAACTTCATCTTAA 
               
               
                   
                 Probe 840 
                 TCGCTAGCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 888 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGAGACACTCTAGCAAA 
               
               
                   
                 Probe 841 
                 ACAGTAAGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 889 
                 Encoding 
                 ATAAGATAGTGAGATGGGAGGAAACTTCATCTTAAT 
               
               
                   
                 Probe 842 
                 CGCTTGCAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 890 
                 Encoding 
                 ATAAGATAGTGAGATGGGCGGGTTACCGGCCCGCCA 
               
               
                   
                 Probe 843 
                 GGGCCTGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 891 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGCCTTTTTCACACACTG 
               
               
                   
                 Probe 844 
                 CCATCGCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 892 
                 Encoding 
                 AATGATATGTTGAGTGGGAGAGACACTCTAGCAAAA 
               
               
                   
                 Probe 845 
                 CAGTAAGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 893 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGAGTTTTTCACACACT 
               
               
                   
                 Probe 846 
                 GCCTACGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 894 
                 Encoding 
                 AATGATATGTTGAGTGGGCCGCGTTACCGGCCCGCC 
               
               
                   
                 Probe 847 
                 AGGGCCTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 895 
                 Encoding 
                 AATGATATGTTGAGTGGGTAGAAAACTTCATCTTAA 
               
               
                   
                 Probe 848 
                 TCGCTAGCGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 896 
                 Encoding 
                 AATGATATGTTGAGTGGGTGAGACACTCTAGCAAAA 
               
               
                   
                 Probe 849 
                 CAGTAAGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 897 
                 Encoding 
                 AATGATATGTTGAGTGGGAGGAAACTTCATCTTAAT 
               
               
                   
                 Probe 850 
                 CGCTTGCAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 898 
                 Encoding 
                 AATGATATGTTGAGTGGGCGGGTTACCGGCCCGCCA 
               
               
                   
                 Probe 851 
                 GGGCCTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 899 
                 Encoding 
                 AATGATATGTTGAGTGGGTGCCTTTTTCACACACTGC 
               
               
                   
                 Probe 852 
                 CATCGCGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 900 
                 Encoding 
                 GGTAATTGAGTAGAAGGGAGTCGGTACCTGCAAACA 
               
               
                   
                 Probe 853 
                 TCCACAGCAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 901 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGGACCCGAAAGATAGG 
               
               
                   
                 Probe 854 
                 CCATGGACGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 902 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCGAGCCGCCGACTGTATA 
               
               
                   
                 Probe 855 
                 TCGGGCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 903 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGACGATGACTTTAAGGA 
               
               
                   
                 Probe 856 
                 TTGGACGCTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 904 
                 Encoding 
                 GGTAATTGAGTAGAAGGGATCCCAATCACCGGTTTC 
               
               
                   
                 Probe 857 
                 ACCGATGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 905 
                 Encoding 
                 GGTAATTGAGTAGAAGGGAACCCACAAAATTTCACG 
               
               
                   
                 Probe 858 
                 GCAGCGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 906 
                 Encoding 
                 GGTAATTGAGTAGAAGGGAATGATAAATCTTTGCTC 
               
               
                   
                 Probe 859 
                 CGACAGTCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 907 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGGCTCTGGATCTTTCCT 
               
               
                   
                 Probe 860 
                 CTGGTTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 908 
                 Encoding 
                 AATGATATGTTGAGTGGGAGTCGGTACCTGCAAACA 
               
               
                   
                 Probe 861 
                 TCCACAGCAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 909 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGACCCGAAAGATAGGC 
               
               
                   
                 Probe 862 
                 CATGGACGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 910 
                 Encoding 
                 AATGATATGTTGAGTGGGCGAGCCGCCGACTGTATA 
               
               
                   
                 Probe 863 
                 TCGGGCGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 911 
                 Encoding 
                 AATGATATGTTGAGTGGGTGACGATGACTTTAAGGA 
               
               
                   
                 Probe 864 
                 TTGGACGCTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 912 
                 Encoding 
                 AATGATATGTTGAGTGGGATCCCAATCACCGGTTTC 
               
               
                   
                 Probe 865 
                 ACCGATGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 913 
                 Encoding 
                 AATGATATGTTGAGTGGGAACCCACAAAATTTCACG 
               
               
                   
                 Probe 866 
                 GCAGCGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 914 
                 Encoding 
                 AATGATATGTTGAGTGGGAATGATAAATCTTTGCTC 
               
               
                   
                 Probe 867 
                 CGACAGTCGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 915 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGCTCTGGATCTTTCCTC 
               
               
                   
                 Probe 868 
                 TGGTTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 916 
                 Encoding 
                 GTAATAGATATGAGGGTGAGTTGGTACATACAAAAT 
               
               
                   
                 Probe 869 
                 GGTATACAATGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 917 
                 Encoding 
                 GTAATAGATATGAGGGTGATGAATGGTATACATACC 
               
               
                   
                 Probe 870 
                 AAACTTTAAAGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 918 
                 Encoding 
                 GTAATAGATATGAGGGTGTGTATGGTATACATACCA 
               
               
                   
                 Probe 871 
                 AACTTTATAGGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 919 
                 Encoding 
                 GTAATAGATATGAGGGTGGTAGGTACATACAAAATG 
               
               
                   
                 Probe 872 
                 GTATACAATGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 920 
                 Encoding 
                 GTAATAGATATGAGGGTGAGTTGGTACATACAAAAT 
               
               
                   
                 Probe 873 
                 GGTATACTATTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 921 
                 Encoding 
                 GTAATAGATATGAGGGTGGTTTGGTATACATACCAA 
               
               
                   
                 Probe 874 
                 ACTTTATTAGGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 922 
                 Encoding 
                 GTAATAGATATGAGGGTGCCAACATACCAAACTTTA 
               
               
                   
                 Probe 875 
                 TTCCCATAATTTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 923 
                 Encoding 
                 TGTATAGGATTAGAAGGGAGTTGGTACATACAAAAT 
               
               
                   
                 Probe 876 
                 GGTATACAATGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 924 
                 Encoding 
                 TGTATAGGATTAGAAGGGATGAATGGTATACATACC 
               
               
                   
                 Probe 877 
                 AAACTTTAAAGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 925 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGTATGGTATACATACCA 
               
               
                   
                 Probe 878 
                 AACTTTATAGGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 926 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGTAGGTACATACAAAAT 
               
               
                   
                 Probe 879 
                 GGTATACAATGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 927 
                 Encoding 
                 TGTATAGGATTAGAAGGGAGTTGGTACATACAAAAT 
               
               
                   
                 Probe 880 
                 GGTATACTATGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 928 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGTTTGGTATACATACCA 
               
               
                   
                 Probe 881 
                 AACTTTATTAGGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 929 
                 Encoding 
                 TGTATAGGATTAGAAGGGCCAACATACCAAACTTTA 
               
               
                   
                 Probe 882 
                 TTCCCATAATTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 930 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGCGTCTCCACTATTGCT 
               
               
                   
                 Probe 883 
                 AGCGCTTGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 931 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGGACTCAACTGTACTCA 
               
               
                   
                 Probe 884 
                 AGGACGCGTCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 932 
                 Encoding 
                 ATAAGATAGTGAGATGGGCAGTTGCAGTTTAGTGAG 
               
               
                   
                 Probe 885 
                 CTGGGAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 933 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGGAATCCATCGAAGACT 
               
               
                   
                 Probe 886 
                 AGGTCCCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 934 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGGATTCATAAAGTACAT 
               
               
                   
                 Probe 887 
                 ACAAAAAGGGTGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 935 
                 Encoding 
                 ATAAGATAGTGAGATGGGAGCATCTCCACTATTGCT 
               
               
                   
                 Probe 888 
                 AGCGCTTGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 936 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGGCACAGCGGTGATTGC 
               
               
                   
                 Probe 889 
                 TCAGACGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 937 
                 Encoding 
                 ATAAGATAGTGAGATGGGACCATTGGCATCCACTTG 
               
               
                   
                 Probe 890 
                 CGTCCAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 938 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGCGTCTCCACTATTGCT 
               
               
                   
                 Probe 891 
                 AGCGCTTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 939 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGGACTCAACTGTACTCA 
               
               
                   
                 Probe 892 
                 AGGACGCGTCGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 940 
                 Encoding 
                 TGTATAGGATTAGAAGGGCAGTTGCAGTTTAGTGAG 
               
               
                   
                 Probe 893 
                 CTGGGAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 941 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGGAATCCATCGAAGACT 
               
               
                   
                 Probe 894 
                 AGGTCCCGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 942 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGGATTCATAAAGTACAT 
               
               
                   
                 Probe 895 
                 ACAAAAAGGGTGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 943 
                 Encoding 
                 TGTATAGGATTAGAAGGGAGCATCTCCACTATTGCT 
               
               
                   
                 Probe 896 
                 AGCGCTTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 944 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGGCACAGCGGTGATTGC 
               
               
                   
                 Probe 897 
                 TCAGACGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 945 
                 Encoding 
                 TGTATAGGATTAGAAGGGACCATTGGCATCCACTTG 
               
               
                   
                 Probe 898 
                 CGTCCAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 946 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGTGTCACTTGGACGAAT 
               
               
                   
                 Probe 899 
                 CCTCGTAGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 947 
                 Encoding 
                 TTAATATGGGTAGTTGGGACGCCCAGCTGTATCATG 
               
               
                   
                 Probe 900 
                 CGGTTAAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 948 
                 Encoding 
                 TTAATATGGGTAGTTGGGCGCTTTGCCTCTCTTTGTT 
               
               
                   
                 Probe 901 
                 GGAGCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 949 
                 Encoding 
                 TTAATATGGGTAGTTGGGACGCCCAGCTGTATCATG 
               
               
                   
                 Probe 902 
                 CGGTAAATGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 950 
                 Encoding 
                 TTAATATGGGTAGTTGGGAGTTGGACGAATCCTCGA 
               
               
                   
                 Probe 903 
                 TCCTTAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 951 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGACTTCACTTGGACGA 
               
               
                   
                 Probe 904 
                 ATCCTGCTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 952 
                 Encoding 
                 TTAATATGGGTAGTTGGGACGCCCAGCTGTATCATG 
               
               
                   
                 Probe 905 
                 CGGATAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 953 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGCACTTTGCCTCTCTTTG 
               
               
                   
                 Probe 906 
                 TTGGAGCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 954 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGTGTCACTTGGACGAAT 
               
               
                   
                 Probe 907 
                 CCTCGTAGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 955 
                 Encoding 
                 TGTATAGGATTAGAAGGGACGCCCAGCTGTATCATG 
               
               
                   
                 Probe 908 
                 CGGTTAAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 956 
                 Encoding 
                 TGTATAGGATTAGAAGGGCGCTTTGCCTCTCTTTGTT 
               
               
                   
                 Probe 909 
                 GGAGCGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 957 
                 Encoding 
                 TGTATAGGATTAGAAGGGACGCCCAGCTGTATCATG 
               
               
                   
                 Probe 910 
                 CGGTAAATGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 958 
                 Encoding 
                 TGTATAGGATTAGAAGGGAGTTGGACGAATCCTCGA 
               
               
                   
                 Probe 911 
                 TCCTTAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 959 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGGACTTCACTTGGACGA 
               
               
                   
                 Probe 912 
                 ATCCTGCTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 960 
                 Encoding 
                 TGTATAGGATTAGAAGGGACGCCCAGCTGTATCATG 
               
               
                   
                 Probe 913 
                 CGGATAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 961 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGCACTTTGCCTCTCTTTG 
               
               
                   
                 Probe 914 
                 TTGGAGCGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 962 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTCGTGACTTTCTAAGTAA 
               
               
                   
                 Probe 915 
                 TTACCGAGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 963 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGTTTCTGATGCAATTCT 
               
               
                   
                 Probe 916 
                 CCGGAACGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 964 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCTTGCTTTAAGAGATCCG 
               
               
                   
                 Probe 917 
                 CTTCGGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 965 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTCTTGCGTCTAGTGTTGT 
               
               
                   
                 Probe 918 
                 TATCGCCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 966 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCTTATCTTTCAAACTCTA 
               
               
                   
                 Probe 919 
                 GACATGGCAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 967 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTCTGCTGACTCCTATAAA 
               
               
                   
                 Probe 920 
                 GGTTTAGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 968 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGGATCTCTTAGGTTTGC 
               
               
                   
                 Probe 921 
                 ACTGCTAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 969 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTCCGAAACCTCCCAACAC 
               
               
                   
                 Probe 922 
                 TTACGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 970 
                 Encoding 
                 TGTATAGGATTAGAAGGGTCGTGACTTTCTAAGTAA 
               
               
                   
                 Probe 923 
                 TTACCGAGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 971 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGTTTCTGATGCAATTCTC 
               
               
                   
                 Probe 924 
                 CGGAACGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 972 
                 Encoding 
                 TGTATAGGATTAGAAGGGCTTGCTTTAAGAGATCCG 
               
               
                   
                 Probe 925 
                 CTTCGGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 973 
                 Encoding 
                 TGTATAGGATTAGAAGGGTCTTGCGTCTAGTGTTGTT 
               
               
                   
                 Probe 926 
                 ATCGCCGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 974 
                 Encoding 
                 TGTATAGGATTAGAAGGGCTTATCTTTCAAACTCTA 
               
               
                   
                 Probe 927 
                 GACATGGCAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 975 
                 Encoding 
                 TGTATAGGATTAGAAGGGTCTGCTGACTCCTATAAA 
               
               
                   
                 Probe 928 
                 GGTTTAGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 976 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGGATCTCTTAGGTTTGC 
               
               
                   
                 Probe 929 
                 ACTGCTAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 977 
                 Encoding 
                 TGTATAGGATTAGAAGGGTCCGAAACCTCCCAACAC 
               
               
                   
                 Probe 930 
                 TTACGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 978 
                 Encoding 
                 GTAATAGATATGAGGGTGGCCACCCTTGGGTCCCCG 
               
               
                   
                 Probe 931 
                 ACACCATCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 979 
                 Encoding 
                 GTAATAGATATGAGGGTGCTCCCTTGGGTCCCCGAC 
               
               
                   
                 Probe 932 
                 ACCATCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 980 
                 Encoding 
                 GTAATAGATATGAGGGTGCCTCCCTTGGGTCCCCGA 
               
               
                   
                 Probe 933 
                 CACGATTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 981 
                 Encoding 
                 GTAATAGATATGAGGGTGCCTCCCTTGGGTCCCCGA 
               
               
                   
                 Probe 934 
                 CACCATCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 982 
                 Encoding 
                 GTAATAGATATGAGGGTGGCCACCCTTGGGTCCCCG 
               
               
                   
                 Probe 935 
                 ACACGATTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 983 
                 Encoding 
                 GTAATAGATATGAGGGTGAAAGGGTTTGCTTACCGT 
               
               
                   
                 Probe 936 
                 CACGCCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 984 
                 Encoding 
                 GTAATAGATATGAGGGTGGCCACCCTTGGGTCCCCG 
               
               
                   
                 Probe 937 
                 ACAGGATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 985 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCCACCCTTGGGTCCCC 
               
               
                   
                 Probe 938 
                 GACACCATCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 986 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGCTCCCTTGGGTCCCCGAC 
               
               
                   
                 Probe 939 
                 ACCATCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 987 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGCCTCCCTTGGGTCCCCGA 
               
               
                   
                 Probe 940 
                 CACGATGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 988 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGCCTCCCTTGGGTCCCCGA 
               
               
                   
                 Probe 941 
                 CACCATCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 989 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCCACCCTTGGGTCCCC 
               
               
                   
                 Probe 942 
                 GACACGATGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 990 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGAAAGGGTTTGCTTACCGT 
               
               
                   
                 Probe 943 
                 CACGCCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 991 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCCACCCTTGGGTCCCC 
               
               
                   
                 Probe 944 
                 GACAGGAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 992 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGTTCAGACCTAAGCAAC 
               
               
                   
                 Probe 945 
                 CGCGACGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 993 
                 Encoding 
                 GGAATTTAGTGAGAAGGGATCGTGACTTTCTGGTTG 
               
               
                   
                 Probe 946 
                 GATACGCAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 994 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTCGCAGTTGCAGACCAGA 
               
               
                   
                 Probe 947 
                 CAGGGCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 995 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGACATAAAGGTTAGG 
               
               
                   
                 Probe 948 
                 CCACCCTGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 996 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCTTGAACGCCTTATCTCT 
               
               
                   
                 Probe 949 
                 AAGGAATGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 997 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTCAGGCCAGTGCGTACGA 
               
               
                   
                 Probe 950 
                 CTTCGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 998 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTTATGGCAACTAGTAACA 
               
               
                   
                 Probe 951 
                 AGGCAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 999 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTTCATCTTTCAAACAAAA 
               
               
                   
                 Probe 952 
                 GCCATGACCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1000 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGAGTTCAGACCTAAGCAA 
               
               
                   
                 Probe 953 
                 CCGCGACGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1001 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGATCGTGACTTTCTGGTTG 
               
               
                   
                 Probe 954 
                 GATACGCAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1002 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTCGCAGTTGCAGACCAG 
               
               
                   
                 Probe 955 
                 ACAGGGCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1003 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGGACATAAAGGTTAGG 
               
               
                   
                 Probe 956 
                 CCACCCTGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1004 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGCTTGAACGCCTTATCTCT 
               
               
                   
                 Probe 957 
                 AAGGAATGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1005 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTCAGGCCAGTGCGTACG 
               
               
                   
                 Probe 958 
                 ACTTCGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1006 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTTATGGCAACTAGTAACA 
               
               
                   
                 Probe 959 
                 AGGCAAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1007 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTTCATCTTTCAAACAAAA 
               
               
                   
                 Probe 960 
                 GCCATGACCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1008 
                 Encoding 
                 TTAATATGGGTAGTTGGGTAATTCCTTTCCCAGCAAG 
               
               
                   
                 Probe 961 
                 CTCCAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1009 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGAACCAGCAAGCTGGTC 
               
               
                   
                 Probe 962 
                 CATTGTAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1010 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGTTCTTGGTAAGGTTCTC 
               
               
                   
                 Probe 963 
                 CGCCAAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1011 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGCCCCATCCCATAGCGA 
               
               
                   
                 Probe 964 
                 TAAAAGAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1012 
                 Encoding 
                 TTAATATGGGTAGTTGGGAAGATCTGACTTGCCCTG 
               
               
                   
                 Probe 965 
                 CCAGGAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1013 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGCTGGACTCCCCGGCTA 
               
               
                   
                 Probe 966 
                 AGGGCGGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1014 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGAGGATTATCTCCGGC 
               
               
                   
                 Probe 967 
                 AGTCAGGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1015 
                 Encoding 
                 TTAATATGGGTAGTTGGGTAGAACTGGGACGGTTTT 
               
               
                   
                 Probe 968 
                 TTTCACGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1016 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTAATTCCTTTCCCAGCAA 
               
               
                   
                 Probe 969 
                 GCTCCAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1017 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGAACCAGCAAGCTGGT 
               
               
                   
                 Probe 970 
                 CCATTGTAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1018 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGTTCTTGGTAAGGTTCT 
               
               
                   
                 Probe 971 
                 CCGCCAAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1019 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCCCCATCCCATAGCGA 
               
               
                   
                 Probe 972 
                 TAAAAGAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1020 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGAAGATCTGACTTGCCCTG 
               
               
                   
                 Probe 973 
                 CCAGGAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1021 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCTGGACTCCCCGGCTA 
               
               
                   
                 Probe 974 
                 AGGGCGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1022 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGGAGGATTATCTCCGGC 
               
               
                   
                 Probe 975 
                 AGTCAGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1023 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTAGAACTGGGACGGTTTT 
               
               
                   
                 Probe 976 
                 TTTCACGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1024 
                 Encoding 
                 GGTAATTGAGTAGAAGGGAAACATCAGACTTAAAA 
               
               
                   
                 Probe 977 
                 GACCGGGAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1025 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGACTCCAAAAGGTTACC 
               
               
                   
                 Probe 978 
                 CCACGCCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1026 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCCGTAAGAGATTTGCTAA 
               
               
                   
                 Probe 979 
                 ACCTCGGCCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1027 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGTTACTCGGTGATAAAG 
               
               
                   
                 Probe 980 
                 AAGTTAGCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1028 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGGACTCTAGGATTGTCA 
               
               
                   
                 Probe 981 
                 AAAGATGTGTTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1029 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTTGTCCAACACTTAGCAT 
               
               
                   
                 Probe 982 
                 TCAAGCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1030 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCGCCCCATCCAAAAGCG 
               
               
                   
                 Probe 983 
                 GTAGGTAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1031 
                 Encoding 
                 GGTAATTGAGTAGAAGGGACCAGATACCGTCGAAA 
               
               
                   
                 Probe 984 
                 CGTGAACAGAATGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1032 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGAAACATCAGACTTAAAA 
               
               
                   
                 Probe 985 
                 GACCGGGAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1033 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGACTCCAAAAGGTTACC 
               
               
                   
                 Probe 986 
                 CCACGCCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1034 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGCCGTAAGAGATTTGCTAA 
               
               
                   
                 Probe 987 
                 ACCTCGGCCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1035 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGTTACTCGGTGATAAAG 
               
               
                   
                 Probe 988 
                 AAGTTAGCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1036 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGGACTCTAGGATTGTCA 
               
               
                   
                 Probe 989 
                 AAAGATGTGTTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1037 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTTGTCCAACACTTAGCAT 
               
               
                   
                 Probe 990 
                 TCAAGCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1038 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGCGCCCCATCCAAAAGCG 
               
               
                   
                 Probe 991 
                 GTAGGTAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1039 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGACCAGATACCGTCGAAA 
               
               
                   
                 Probe 992 
                 CGTGAACAGAATGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1040 
                 Encoding 
                 GTAATAGATATGAGGGTGGTGGTCTATATGTCCCGA 
               
               
                   
                 Probe 993 
                 AGGTTCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1041 
                 Encoding 
                 GTAATAGATATGAGGGTGGATTTAATATTGGCAACC 
               
               
                   
                 Probe 994 
                 GGAGTATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1042 
                 Encoding 
                 GTAATAGATATGAGGGTGGCTTACCGTCATTCTTCAT 
               
               
                   
                 Probe 995 
                 CCGAGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1043 
                 Encoding 
                 GTAATAGATATGAGGGTGGATTGTTATCCCGATGAC 
               
               
                   
                 Probe 996 
                 AGACCGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1044 
                 Encoding 
                 GTAATAGATATGAGGGTGGACCAGTAACCTTTTTAC 
               
               
                   
                 Probe 997 
                 CCCATACTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1045 
                 Encoding 
                 GTAATAGATATGAGGGTGCAATACCCCCTTCGTCTA 
               
               
                   
                 Probe 998 
                 GTAAGGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1046 
                 Encoding 
                 GTAATAGATATGAGGGTGGGTCATCGGTTTTACCTT 
               
               
                   
                 Probe 999 
                 CGGGCCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1047 
                 Encoding 
                 GTAATAGATATGAGGGTGCGACCAGTTTTATGTGCA 
               
               
                   
                 Probe 1000 
                 ATTCCCGCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1048 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGTGGTCTATATGTCCCG 
               
               
                   
                 Probe 1001 
                 AAGGTTCGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1049 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGATTTAATATTGGCAAC 
               
               
                   
                 Probe 1002 
                 CGGAGTAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1050 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGCTTACCGTCATTCTTCA 
               
               
                   
                 Probe 1003 
                 TCCGAGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1051 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGATTGTTATCCCGATGA 
               
               
                   
                 Probe 1004 
                 CAGACCGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1052 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGACCAGTAACCTTTTTA 
               
               
                   
                 Probe 1005 
                 CCCCATACGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1053 
                 Encoding 
                 GGATAGAGTATAGTTGGGCAATACCCCCTTCGTCTA 
               
               
                   
                 Probe 1006 
                 GTAAGGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1054 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGGTCATCGGTTTTACCTT 
               
               
                   
                 Probe 1007 
                 CGGGCCGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1055 
                 Encoding 
                 GGATAGAGTATAGTTGGGCGACCAGTTTTATGTGCA 
               
               
                   
                 Probe 1008 
                 ATTCCCGCGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1056 
                 Encoding 
                 ATAAGATAGTGAGATGGGAGTCGCGACCCTTCCTCC 
               
               
                   
                 Probe 1009 
                 CGATCCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1057 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGACAGAAGTTTACGTAC 
               
               
                   
                 Probe 1010 
                 CGAAAATGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1058 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGTAGGCCAAGAGGAAT 
               
               
                   
                 Probe 1011 
                 CATGCCCAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1059 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGGTCGGCCAAGAGGAA 
               
               
                   
                 Probe 1012 
                 TCATGCCCAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1060 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGAGTCGCGACCCTTCCT 
               
               
                   
                 Probe 1013 
                 CCCGTTCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1061 
                 Encoding 
                 ATAAGATAGTGAGATGGGACGATAGAAGTTTACGTA 
               
               
                   
                 Probe 1014 
                 CCGAATATGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1062 
                 Encoding 
                 ATAAGATAGTGAGATGGGAGCTGCCGGGCAGATGTC 
               
               
                   
                 Probe 1015 
                 AAGCTGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1063 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGAGCTGCCGGGCAGAT 
               
               
                   
                 Probe 1016 
                 GTCAACCTGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1064 
                 Encoding 
                 GGATAGAGTATAGTTGGGAGTCGCGACCCTTCCTCC 
               
               
                   
                 Probe 1017 
                 CGATCCGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1065 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGACAGAAGTTTACGTAC 
               
               
                   
                 Probe 1018 
                 CGAAAATGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1066 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGTAGGCCAAGAGGAATC 
               
               
                   
                 Probe 1019 
                 ATGCCCAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1067 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGGTCGGCCAAGAGGAAT 
               
               
                   
                 Probe 1020 
                 CATGCCCAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1068 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGAGTCGCGACCCTTCCT 
               
               
                   
                 Probe 1021 
                 CCCGTTCGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1069 
                 Encoding 
                 GGATAGAGTATAGTTGGGACGATAGAAGTTTACGTA 
               
               
                   
                 Probe 1022 
                 CCGAATATGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1070 
                 Encoding 
                 GGATAGAGTATAGTTGGGAGCTGCCGGGCAGATGTC 
               
               
                   
                 Probe 1023 
                 AAGCTGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1071 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGAGCTGCCGGGCAGATG 
               
               
                   
                 Probe 1024 
                 TCAACCTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1072 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCGTGGAGGGTCCATACCC 
               
               
                   
                 Probe 1025 
                 TCCCTGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1073 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCCGCGGAGGGTCCATACC 
               
               
                   
                 Probe 1026 
                 CTCCGTGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1074 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGCCCCGGAGGGTCCATA 
               
               
                   
                 Probe 1027 
                 CCCTCGCTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1075 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGCCCCGGAGGGTCCATA 
               
               
                   
                 Probe 1028 
                 CCCTCCCTGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1076 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCCGCGGAGGGTCCATACC 
               
               
                   
                 Probe 1029 
                 CTCGCTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1077 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCGTGGAGGGTCCATACCC 
               
               
                   
                 Probe 1030 
                 TCCGTGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1078 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGTGGAGGGTCCATACCC 
               
               
                   
                 Probe 1031 
                 TCCGTGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1079 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCCGCGGAGGGTCCATACC 
               
               
                   
                 Probe 1032 
                 CTCCGTGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1080 
                 Encoding 
                 GGATAGAGTATAGTTGGGCGTGGAGGGTCCATACCC 
               
               
                   
                 Probe 1033 
                 TCCCTGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1081 
                 Encoding 
                 GGATAGAGTATAGTTGGGCCGCGGAGGGTCCATACC 
               
               
                   
                 Probe 1034 
                 CTCCGTGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1082 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGCCCCGGAGGGTCCATA 
               
               
                   
                 Probe 1035 
                 CCCTCGCTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1083 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGCCCCGGAGGGTCCATA 
               
               
                   
                 Probe 1036 
                 CCCTCCCTGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1084 
                 Encoding 
                 GGATAGAGTATAGTTGGGCCGCGGAGGGTCCATACC 
               
               
                   
                 Probe 1037 
                 CTCGCTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1085 
                 Encoding 
                 GGATAGAGTATAGTTGGGCGTGGAGGGTCCATACCC 
               
               
                   
                 Probe 1038 
                 TCCGTGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1086 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGTGGAGGGTCCATACCC 
               
               
                   
                 Probe 1039 
                 TCCGTGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1087 
                 Encoding 
                 GGATAGAGTATAGTTGGGCCGCGGAGGGTCCATACC 
               
               
                   
                 Probe 1040 
                 CTCCCTGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1088 
                 Encoding 
                 GTAATAGATATGAGGGTGGAGCGGCACTCTAGAAA 
               
               
                   
                 Probe 1041 
                 AACAGAAATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1089 
                 Encoding 
                 GTAATAGATATGAGGGTGAATTTTGGGATTTGCTAG 
               
               
                   
                 Probe 1042 
                 GCAAGCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1090 
                 Encoding 
                 GTAATAGATATGAGGGTGGAGCGGCACTCTAGAAA 
               
               
                   
                 Probe 1043 
                 AACACAATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1091 
                 Encoding 
                 GTAATAGATATGAGGGTGAGTCCGAAGAGATCATCT 
               
               
                   
                 Probe 1044 
                 TAAATGGAATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1092 
                 Encoding 
                 GTAATAGATATGAGGGTGCAATTTTGGGATTTGCTA 
               
               
                   
                 Probe 1045 
                 GGCTAGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1093 
                 Encoding 
                 GTAATAGATATGAGGGTGGAGCGGCACTCTAGAAA 
               
               
                   
                 Probe 1046 
                 AACAGTAAGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1094 
                 Encoding 
                 GTAATAGATATGAGGGTGGCGAGTCATATAAGACTC 
               
               
                   
                 Probe 1047 
                 AATCCGTTCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1095 
                 Encoding 
                 GTAATAGATATGAGGGTGCGAGTCATATAAGACTCA 
               
               
                   
                 Probe 1048 
                 ATCCGTTCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1096 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGAGCGGCACTCTAGAAA 
               
               
                   
                 Probe 1049 
                 AACAGAAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1097 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAATTTTGGGATTTGCTAG 
               
               
                   
                 Probe 1050 
                 GCAAGCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1098 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGAGCGGCACTCTAGAAA 
               
               
                   
                 Probe 1051 
                 AACACAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1099 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAGTCCGAAGAGATCATCT 
               
               
                   
                 Probe 1052 
                 TAAATGGAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1100 
                 Encoding 
                 TGTAATAGTAAGGAGGGACAATTTTGGGATTTGCTA 
               
               
                   
                 Probe 1053 
                 GGCTAGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1101 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGAGCGGCACTCTAGAAA 
               
               
                   
                 Probe 1054 
                 AACAGTAAGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1102 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGCGAGTCATATAAGACTC 
               
               
                   
                 Probe 1055 
                 AATCCGTTCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1103 
                 Encoding 
                 TGTAATAGTAAGGAGGGACGAGTCATATAAGACTCA 
               
               
                   
                 Probe 1056 
                 ATCCGTTCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1104 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGTCGCGGGCTCATCTTA 
               
               
                   
                 Probe 1057 
                 TACTTGGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1105 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTTTTCCTCAAAATCGCTT 
               
               
                   
                 Probe 1058 
                 CGCAGCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1106 
                 Encoding 
                 GGAATTTAGTGAGAAGGGATTCCCTGCCTTTCACTTC 
               
               
                   
                 Probe 1059 
                 AGTGAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1107 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAACCCAGATTACTCCTTT 
               
               
                   
                 Probe 1060 
                 GCCAGGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1108 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCCAGGGAGATGTCAAGA 
               
               
                   
                 Probe 1061 
                 CTTGCATGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1109 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCGTTTCCAAAGCAGTTCA 
               
               
                   
                 Probe 1062 
                 GGGCAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1110 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTTTTTCCTCAAAATCGCT 
               
               
                   
                 Probe 1063 
                 TCGGAGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1111 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGTCGCGGGCTCATCTTA 
               
               
                   
                 Probe 1064 
                 TACTTGGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1112 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGTCGCGGGCTCATCTTAT 
               
               
                   
                 Probe 1065 
                 ACTTGGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1113 
                 Encoding 
                 TGTAATAGTAAGGAGGGATTTTCCTCAAAATCGCTT 
               
               
                   
                 Probe 1066 
                 CGCAGCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1114 
                 Encoding 
                 TGTAATAGTAAGGAGGGAATTCCCTGCCTTTCACTTC 
               
               
                   
                 Probe 1067 
                 AGTGAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1115 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAACCCAGATTACTCCTTT 
               
               
                   
                 Probe 1068 
                 GCCAGGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1116 
                 Encoding 
                 TGTAATAGTAAGGAGGGACCAGGGAGATGTCAAGA 
               
               
                   
                 Probe 1069 
                 CTTGCATGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1117 
                 Encoding 
                 TGTAATAGTAAGGAGGGACGTTTCCAAAGCAGTTCA 
               
               
                   
                 Probe 1070 
                 GGGCAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1118 
                 Encoding 
                 TGTAATAGTAAGGAGGGATTTTTCCTCAAAATCGCT 
               
               
                   
                 Probe 1071 
                 TCGGAGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1119 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAGTCGCGGGCTCATCTTA 
               
               
                   
                 Probe 1072 
                 TACTTGGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1120 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGTAGAAAACTTCCGTAC 
               
               
                   
                 Probe 1073 
                 TAAGACAGGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1121 
                 Encoding 
                 ATAAGATAGTGAGATGGGTAAATGGAAATATCATGC 
               
               
                   
                 Probe 1074 
                 GGTTAGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1122 
                 Encoding 
                 ATAAGATAGTGAGATGGGAACGGAAATATCATGCG 
               
               
                   
                 Probe 1075 
                 GTATCAGGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1123 
                 Encoding 
                 ATAAGATAGTGAGATGGGTTGCCGTACTAAGACCCC 
               
               
                   
                 Probe 1076 
                 GTTGCTGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1124 
                 Encoding 
                 ATAAGATAGTGAGATGGGAAACTTCTGACTTGCATG 
               
               
                   
                 Probe 1077 
                 GCCCGGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1125 
                 Encoding 
                 ATAAGATAGTGAGATGGGAGGAAACTTCCGTACTAA 
               
               
                   
                 Probe 1078 
                 GACCGGCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1126 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGGTCGAAAACTTCCGTA 
               
               
                   
                 Probe 1079 
                 CTAAGTGGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1127 
                 Encoding 
                 ATAAGATAGTGAGATGGGATAGATGGAAATATCATG 
               
               
                   
                 Probe 1080 
                 CGGATAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1128 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGTAGAAAACTTCCGTACT 
               
               
                   
                 Probe 1081 
                 AAGACAGGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1129 
                 Encoding 
                 TGTAATAGTAAGGAGGGATAAATGGAAATATCATGC 
               
               
                   
                 Probe 1082 
                 GGTTAGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1130 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAACGGAAATATCATGCG 
               
               
                   
                 Probe 1083 
                 GTATCAGGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1131 
                 Encoding 
                 TGTAATAGTAAGGAGGGATTGCCGTACTAAGACCCC 
               
               
                   
                 Probe 1084 
                 GTTGCTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1132 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAAACTTCTGACTTGCATG 
               
               
                   
                 Probe 1085 
                 GCCCGGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1133 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAGGAAACTTCCGTACTAA 
               
               
                   
                 Probe 1086 
                 GACCGGCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1134 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGGTCGAAAACTTCCGTAC 
               
               
                   
                 Probe 1087 
                 TAAGTGGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1135 
                 Encoding 
                 TGTAATAGTAAGGAGGGAATAGATGGAAATATCATG 
               
               
                   
                 Probe 1088 
                 CGGATAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1136 
                 Encoding 
                 TTAATATGGGTAGTTGGGTCGTGCGACTCAGCTGCA 
               
               
                   
                 Probe 1089 
                 TTATCGCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1137 
                 Encoding 
                 TTAATATGGGTAGTTGGGCTCATGCGACTCAGCTGC 
               
               
                   
                 Probe 1090 
                 ATTTACGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1138 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGTCGACTCAGCTGCAT 
               
               
                   
                 Probe 1091 
                 TATGCCCAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1139 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGTAGACTCAGCTGCATT 
               
               
                   
                 Probe 1092 
                 ATGCCCAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1140 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGTCGACTCAGCTGCAT 
               
               
                   
                 Probe 1093 
                 TATGGCCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1141 
                 Encoding 
                 TTAATATGGGTAGTTGGGTCGTGCGACTCAGCTGCA 
               
               
                   
                 Probe 1094 
                 TTAACGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1142 
                 Encoding 
                 TTAATATGGGTAGTTGGGCGGGCGACTCAGCTGCAT 
               
               
                   
                 Probe 1095 
                 TATCGCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1143 
                 Encoding 
                 TTAATATGGGTAGTTGGGCGGGCGACTCAGCTGCAT 
               
               
                   
                 Probe 1096 
                 TATGGCCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1144 
                 Encoding 
                 TGTAATAGTAAGGAGGGATCGTGCGACTCAGCTGCA 
               
               
                   
                 Probe 1097 
                 TTATCGCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1145 
                 Encoding 
                 TGTAATAGTAAGGAGGGACTCATGCGACTCAGCTGC 
               
               
                   
                 Probe 1098 
                 ATTTACGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1146 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGGTCGACTCAGCTGCATT 
               
               
                   
                 Probe 1099 
                 ATGCCCAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1147 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGTAGACTCAGCTGCATTA 
               
               
                   
                 Probe 1100 
                 TGCCCAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1148 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGGTCGACTCAGCTGCATT 
               
               
                   
                 Probe 1101 
                 ATGGCCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1149 
                 Encoding 
                 TGTAATAGTAAGGAGGGATCGTGCGACTCAGCTGCA 
               
               
                   
                 Probe 1102 
                 TTAACGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1150 
                 Encoding 
                 TGTAATAGTAAGGAGGGACGGGCGACTCAGCTGCAT 
               
               
                   
                 Probe 1103 
                 TATCGCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1151 
                 Encoding 
                 TGTAATAGTAAGGAGGGACGGGCGACTCAGCTGCAT 
               
               
                   
                 Probe 1104 
                 TATGGCCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1152 
                 Encoding 
                 GTAATAGATATGAGGGTGGTCCCATCCATATCCACA 
               
               
                   
                 Probe 1105 
                 GCTCAGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1153 
                 Encoding 
                 GTAATAGATATGAGGGTGGACGCACTGAATTCTCTC 
               
               
                   
                 Probe 1106 
                 CAAGTGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1154 
                 Encoding 
                 GTAATAGATATGAGGGTGAAATCTTACAACAGAGCT 
               
               
                   
                 Probe 1107 
                 TTACGATGGCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1155 
                 Encoding 
                 GTAATAGATATGAGGGTGGCTGCTTTTACTTCAGAC 
               
               
                   
                 Probe 1108 
                 TTATACAAGGCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1156 
                 Encoding 
                 GTAATAGATATGAGGGTGGTCAGCTGTGAAATGTAC 
               
               
                   
                 Probe 1109 
                 TCCCAATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1157 
                 Encoding 
                 GTAATAGATATGAGGGTGAGAAGGGCCTTTATTGCC 
               
               
                   
                 Probe 1110 
                 ATGAGTTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1158 
                 Encoding 
                 GTAATAGATATGAGGGTGAAAGTTCCGCTTACAATC 
               
               
                   
                 Probe 1111 
                 TCTTCGATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1159 
                 Encoding 
                 GTAATAGATATGAGGGTGCTGCTCACTCCCGTAGGT 
               
               
                   
                 Probe 1112 
                 TGTGCGTGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1160 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGTCCCATCCATATCCAC 
               
               
                   
                 Probe 1113 
                 AGCTCAGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1161 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGACGCACTGAATTCTCT 
               
               
                   
                 Probe 1114 
                 CCAAGTGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1162 
                 Encoding 
                 TATAGTTATGGAGAAGGGAAATCTTACAACAGAGCT 
               
               
                   
                 Probe 1115 
                 TTACGATGGCGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1163 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGCTGCTTTTACTTCAGA 
               
               
                   
                 Probe 1116 
                 CTTATACAAGGCGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1164 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGTCAGCTGTGAAATGTA 
               
               
                   
                 Probe 1117 
                 CTCCCAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1165 
                 Encoding 
                 TATAGTTATGGAGAAGGGAGAAGGGCCTTTATTGCC 
               
               
                   
                 Probe 1118 
                 ATGAGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1166 
                 Encoding 
                 TATAGTTATGGAGAAGGGAAAGTTCCGCTTACAATC 
               
               
                   
                 Probe 1119 
                 TCTTCGAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1167 
                 Encoding 
                 TATAGTTATGGAGAAGGGCTGCTCACTCCCGTAGGT 
               
               
                   
                 Probe 1120 
                 TGTGCGTGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1168 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGAGTCAGGTACTGTCAC 
               
               
                   
                 Probe 1121 
                 TTTCAAGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1169 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGAATCAGGTACTGTCAC 
               
               
                   
                 Probe 1122 
                 TTTCAAGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1170 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAATCAGGTACTGTCACTT 
               
               
                   
                 Probe 1123 
                 TCTTAGGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1171 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCCCTCTTAGTCAGGTACT 
               
               
                   
                 Probe 1124 
                 GTCACAAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1172 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGAGTCAGGTACTGTCAC 
               
               
                   
                 Probe 1125 
                 TTTCTAGGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1173 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGAGTCAGGTACTGTCAC 
               
               
                   
                 Probe 1126 
                 TTTGAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1174 
                 Encoding 
                 GGAATTTAGTGAGAAGGGATCAGGTACTGTCACTTT 
               
               
                   
                 Probe 1127 
                 CTTCGGAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1175 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGAATCAGGTACTGTCAC 
               
               
                   
                 Probe 1128 
                 TTTCTAGGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1176 
                 Encoding 
                 TATAGTTATGGAGAAGGGAGAGTCAGGTACTGTCAC 
               
               
                   
                 Probe 1129 
                 TTTCAAGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1177 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGAATCAGGTACTGTCAC 
               
               
                   
                 Probe 1130 
                 TTTCAAGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1178 
                 Encoding 
                 TATAGTTATGGAGAAGGGAATCAGGTACTGTCACTT 
               
               
                   
                 Probe 1131 
                 TCTTAGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1179 
                 Encoding 
                 TATAGTTATGGAGAAGGGCCCTCTTAGTCAGGTACT 
               
               
                   
                 Probe 1132 
                 GTCACAAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1180 
                 Encoding 
                 TATAGTTATGGAGAAGGGAGAGTCAGGTACTGTCAC 
               
               
                   
                 Probe 1133 
                 TTTCTAGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1181 
                 Encoding 
                 TATAGTTATGGAGAAGGGAGAGTCAGGTACTGTCAC 
               
               
                   
                 Probe 1134 
                 TTTGAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1182 
                 Encoding 
                 TATAGTTATGGAGAAGGGATCAGGTACTGTCACTTT 
               
               
                   
                 Probe 1135 
                 CTTCGGAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1183 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGAATCAGGTACTGTCAC 
               
               
                   
                 Probe 1136 
                 TTTCTAGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1184 
                 Encoding 
                 ATAAGATAGTGAGATGGGATCAGTTCGTTATGCAAT 
               
               
                   
                 Probe 1137 
                 CCTGTCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1185 
                 Encoding 
                 ATAAGATAGTGAGATGGGCGAGGCACCGAGGATTC 
               
               
                   
                 Probe 1138 
                 CTCCCGCTGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1186 
                 Encoding 
                 ATAAGATAGTGAGATGGGTACGGCCCATCTTTTACC 
               
               
                   
                 Probe 1139 
                 GAATATTAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1187 
                 Encoding 
                 ATAAGATAGTGAGATGGGCGACAATTATTTTCGCTC 
               
               
                   
                 Probe 1140 
                 GACTTCGTGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1188 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGACACTGGGTTTTTGTG 
               
               
                   
                 Probe 1141 
                 CTTTCGAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1189 
                 Encoding 
                 ATAAGATAGTGAGATGGGCTAGGGCCCATCTTTTAC 
               
               
                   
                 Probe 1142 
                 CGAATATTAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1190 
                 Encoding 
                 ATAAGATAGTGAGATGGGCGAACTTTGTTTCCAGCC 
               
               
                   
                 Probe 1143 
                 ATTCATGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1191 
                 Encoding 
                 ATAAGATAGTGAGATGGGCGACAATTATTTTCGCTC 
               
               
                   
                 Probe 1144 
                 GACTACGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1192 
                 Encoding 
                 TATAGTTATGGAGAAGGGATCAGTTCGTTATGCAAT 
               
               
                   
                 Probe 1145 
                 CCTGTCGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1193 
                 Encoding 
                 TATAGTTATGGAGAAGGGCGAGGCACCGAGGATTCC 
               
               
                   
                 Probe 1146 
                 TCCCGCTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1194 
                 Encoding 
                 TATAGTTATGGAGAAGGGTACGGCCCATCTTTTACC 
               
               
                   
                 Probe 1147 
                 GAATATTAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1195 
                 Encoding 
                 TATAGTTATGGAGAAGGGCGACAATTATTTTCGCTC 
               
               
                   
                 Probe 1148 
                 GACTTCGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1196 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGACACTGGGTTTTTGTG 
               
               
                   
                 Probe 1149 
                 CTTTCGAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1197 
                 Encoding 
                 TATAGTTATGGAGAAGGGCTAGGGCCCATCTTTTAC 
               
               
                   
                 Probe 1150 
                 CGAATATTAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1198 
                 Encoding 
                 TATAGTTATGGAGAAGGGCGAACTTTGTTTCCAGCC 
               
               
                   
                 Probe 1151 
                 ATTCATGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1199 
                 Encoding 
                 TATAGTTATGGAGAAGGGCGACAATTATTTTCGCTC 
               
               
                   
                 Probe 1152 
                 GACTACGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1200 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGTGGGAATTCCGATCTC 
               
               
                   
                 Probe 1153 
                 CCCTTGGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1201 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGTGGGAATTCCGATCTC 
               
               
                   
                 Probe 1154 
                 CCCTAGGCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1202 
                 Encoding 
                 TTAATATGGGTAGTTGGGATGCGCCTACTACCTAAT 
               
               
                   
                 Probe 1155 
                 GGGCGCGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1203 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGTGGGAATTCCGATCTC 
               
               
                   
                 Probe 1156 
                 CCCATGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1204 
                 Encoding 
                 TTAATATGGGTAGTTGGGATACGCCTACTACCTAAT 
               
               
                   
                 Probe 1157 
                 GGGAGCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1205 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGTGGGAATTCCGATCTC 
               
               
                   
                 Probe 1158 
                 CCCTTGGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1206 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGGCCTACTACCTAATG 
               
               
                   
                 Probe 1159 
                 GGCCCGCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1207 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGGCCTACTACCTAATG 
               
               
                   
                 Probe 1160 
                 GGCGCGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1208 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGTGGGAATTCCGATCTC 
               
               
                   
                 Probe 1161 
                 CCCTTGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1209 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGTGGGAATTCCGATCTC 
               
               
                   
                 Probe 1162 
                 CCCTAGGCGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1210 
                 Encoding 
                 TATAGTTATGGAGAAGGGATGCGCCTACTACCTAAT 
               
               
                   
                 Probe 1163 
                 GGGCGCGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1211 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGTGGGAATTCCGATCTC 
               
               
                   
                 Probe 1164 
                 CCCATGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1212 
                 Encoding 
                 TATAGTTATGGAGAAGGGATACGCCTACTACCTAAT 
               
               
                   
                 Probe 1165 
                 GGGAGCGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1213 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGTGGGAATTCCGATCTC 
               
               
                   
                 Probe 1166 
                 CCCTTGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1214 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGGGCCTACTACCTAATG 
               
               
                   
                 Probe 1167 
                 GGCCCGCGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1215 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGGGCCTACTACCTAATG 
               
               
                   
                 Probe 1168 
                 GGCGCGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1216 
                 Encoding 
                 GGTAATTGAGTAGAAGGGATACACCCTAATTACCAG 
               
               
                   
                 Probe 1169 
                 TCCATGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1217 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGTCGCGAGCTCATCTTT 
               
               
                   
                 Probe 1170 
                 GGACCTAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1218 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCAAGTCCCCGATTAAAGA 
               
               
                   
                 Probe 1171 
                 TCTTATGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1219 
                 Encoding 
                 GGTAATTGAGTAGAAGGGATTCCCCAGATTTCACTT 
               
               
                   
                 Probe 1172 
                 CTGTGAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1220 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCCTGGGTCAATACCTCCC 
               
               
                   
                 Probe 1173 
                 ACAGGAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1221 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGGATGTATCAACTAACC 
               
               
                   
                 Probe 1174 
                 GTAAGGCAAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1222 
                 Encoding 
                 GGTAATTGAGTAGAAGGGAAATTCCCTCTGTATGAC 
               
               
                   
                 Probe 1175 
                 TGCGTAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1223 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGCCGTTATCCCCCATCC 
               
               
                   
                 Probe 1176 
                 AAAGCGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1224 
                 Encoding 
                 TATAGTTATGGAGAAGGGATACACCCTAATTACCAG 
               
               
                   
                 Probe 1177 
                 TCCATGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1225 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGTCGCGAGCTCATCTTT 
               
               
                   
                 Probe 1178 
                 GGACCTAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1226 
                 Encoding 
                 TATAGTTATGGAGAAGGGCAAGTCCCCGATTAAAGA 
               
               
                   
                 Probe 1179 
                 TCTTATGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1227 
                 Encoding 
                 TATAGTTATGGAGAAGGGATTCCCCAGATTTCACTT 
               
               
                   
                 Probe 1180 
                 CTGTGAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1228 
                 Encoding 
                 TATAGTTATGGAGAAGGGCCTGGGTCAATACCTCCC 
               
               
                   
                 Probe 1181 
                 ACAGGAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1229 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGGATGTATCAACTAACC 
               
               
                   
                 Probe 1182 
                 GTAAGGCAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1230 
                 Encoding 
                 TATAGTTATGGAGAAGGGAAATTCCCTCTGTATGAC 
               
               
                   
                 Probe 1183 
                 TGCGTAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1231 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGCCGTTATCCCCCATCC 
               
               
                   
                 Probe 1184 
                 AAAGCGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1232 
                 Encoding 
                 GTAATAGATATGAGGGTGCGACTCTTTACAGTTGGC 
               
               
                   
                 Probe 1185 
                 TCAGTCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1233 
                 Encoding 
                 GTAATAGATATGAGGGTGAAGATCACTGTGTTGCTT 
               
               
                   
                 Probe 1186 
                 CCCAGATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1234 
                 Encoding 
                 GTAATAGATATGAGGGTGTAGGCGATAAAATTAGTA 
               
               
                   
                 Probe 1187 
                 TATGCGCATTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1235 
                 Encoding 
                 GTAATAGATATGAGGGTGGAAAAAGTAAACTTTCGA 
               
               
                   
                 Probe 1188 
                 TTAAGTTCCAATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1236 
                 Encoding 
                 GTAATAGATATGAGGGTGACGCCTCTTTACAGTTGG 
               
               
                   
                 Probe 1189 
                 CTCTGTTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1237 
                 Encoding 
                 GTAATAGATATGAGGGTGGAACATCACTGTGTTGCT 
               
               
                   
                 Probe 1190 
                 TCCGAGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1238 
                 Encoding 
                 GTAATAGATATGAGGGTGATATGCGATAAAATTAGT 
               
               
                   
                 Probe 1191 
                 ATATGCGCATTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1239 
                 Encoding 
                 GTAATAGATATGAGGGTGGTTGTAAACTTTCGATTA 
               
               
                   
                 Probe 1192 
                 AGTTCGAAGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1240 
                 Encoding 
                 GATAAGTAAGTAGGGATGCGACTCTTTACAGTTGGC 
               
               
                   
                 Probe 1193 
                 TCAGTCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1241 
                 Encoding 
                 GATAAGTAAGTAGGGATGAAGATCACTGTGTTGCTT 
               
               
                   
                 Probe 1194 
                 CCCAGAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1242 
                 Encoding 
                 GATAAGTAAGTAGGGATGTAGGCGATAAAATTAGTA 
               
               
                   
                 Probe 1195 
                 TATGCGCATGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1243 
                 Encoding 
                 GATAAGTAAGTAGGGATGGAAAAAGTAAACTTTCG 
               
               
                   
                 Probe 1196 
                 ATTAAGTTCCAAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1244 
                 Encoding 
                 GATAAGTAAGTAGGGATGACGCCTCTTTACAGTTGG 
               
               
                   
                 Probe 1197 
                 CTCTGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1245 
                 Encoding 
                 GATAAGTAAGTAGGGATGGAACATCACTGTGTTGCT 
               
               
                   
                 Probe 1198 
                 TCCGAGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1246 
                 Encoding 
                 GATAAGTAAGTAGGGATGATATGCGATAAAATTAGT 
               
               
                   
                 Probe 1199 
                 ATATGCGCATGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1247 
                 Encoding 
                 GATAAGTAAGTAGGGATGGTTGTAAACTTTCGATTA 
               
               
                   
                 Probe 1200 
                 AGTTCGAAGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1248 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTCCGATGTCAAGGACTGG 
               
               
                   
                 Probe 1201 
                 TAAGCAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1249 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGCCTCGCCTCACTCTGT 
               
               
                   
                 Probe 1202 
                 TGGCTGGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1250 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGTCGGATGTCAAGGACT 
               
               
                   
                 Probe 1203 
                 GGTATCCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1251 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCGGGCAGGCTTATGCGGT 
               
               
                   
                 Probe 1204 
                 ATTTCGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1252 
                 Encoding 
                 GGAATTTAGTGAGAAGGGACGTCTTCCCTCCGGAGA 
               
               
                   
                 Probe 1205 
                 GTTCCGAGCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1253 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGACCTCCGGAGAGTTCC 
               
               
                   
                 Probe 1206 
                 GTCCCGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1254 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGAGTTATCGAGCCTGC 
               
               
                   
                 Probe 1207 
                 CTTGCTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1255 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGACAGGCTTATGCGGT 
               
               
                   
                 Probe 1208 
                 ATTACGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1256 
                 Encoding 
                 GATAAGTAAGTAGGGATGTCCGATGTCAAGGACTGG 
               
               
                   
                 Probe 1209 
                 TAAGCAAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1257 
                 Encoding 
                 GATAAGTAAGTAGGGATGGCCTCGCCTCACTCTGTT 
               
               
                   
                 Probe 1210 
                 GGCTGGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1258 
                 Encoding 
                 GATAAGTAAGTAGGGATGGTCGGATGTCAAGGACTG 
               
               
                   
                 Probe 1211 
                 GTATCCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1259 
                 Encoding 
                 GATAAGTAAGTAGGGATGCGGGCAGGCTTATGCGGT 
               
               
                   
                 Probe 1212 
                 ATTTCGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1260 
                 Encoding 
                 GATAAGTAAGTAGGGATGACGTCTTCCCTCCGGAGA 
               
               
                   
                 Probe 1213 
                 GTTCCGAGCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1261 
                 Encoding 
                 GATAAGTAAGTAGGGATGAGACCTCCGGAGAGTTCC 
               
               
                   
                 Probe 1214 
                 GTCCCGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1262 
                 Encoding 
                 GATAAGTAAGTAGGGATGTGGAGTTATCGAGCCTGC 
               
               
                   
                 Probe 1215 
                 CTTGCTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1263 
                 Encoding 
                 GATAAGTAAGTAGGGATGGGACAGGCTTATGCGGTA 
               
               
                   
                 Probe 1216 
                 TTACGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1264 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGGCTCAGTTTTTACCCC 
               
               
                   
                 Probe 1217 
                 TGTTGGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1265 
                 Encoding 
                 ATAAGATAGTGAGATGGGCTGCTCCCTCCTGGTTAG 
               
               
                   
                 Probe 1218 
                 GTTCCCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1266 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGACGTGGTCGCTTCTCT 
               
               
                   
                 Probe 1219 
                 TTGAAAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1267 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGTACCTCAGTTTTTACC 
               
               
                   
                 Probe 1220 
                 CCTGATGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1268 
                 Encoding 
                 ATAAGATAGTGAGATGGGCTGCTCCCTCCTGGTTAG 
               
               
                   
                 Probe 1221 
                 GTTGCCAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1269 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGTGCCTCAGTTTTTACC 
               
               
                   
                 Probe 1222 
                 CCTGATGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1270 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGTACCTCAGTTTTTACC 
               
               
                   
                 Probe 1223 
                 CCTCATGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1271 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGGACTGGTTAGGTTGGG 
               
               
                   
                 Probe 1224 
                 TCACGCCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1272 
                 Encoding 
                 GATAAGTAAGTAGGGATGTGGCTCAGTTTTTACCCC 
               
               
                   
                 Probe 1225 
                 TGTTGGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1273 
                 Encoding 
                 GATAAGTAAGTAGGGATGCTGCTCCCTCCTGGTTAG 
               
               
                   
                 Probe 1226 
                 GTTCCCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1274 
                 Encoding 
                 GATAAGTAAGTAGGGATGTGACGTGGTCGCTTCTCT 
               
               
                   
                 Probe 1227 
                 TTGAAAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1275 
                 Encoding 
                 GATAAGTAAGTAGGGATGTGTACCTCAGTTTTTACC 
               
               
                   
                 Probe 1228 
                 CCTGATGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1276 
                 Encoding 
                 GATAAGTAAGTAGGGATGCTGCTCCCTCCTGGTTAG 
               
               
                   
                 Probe 1229 
                 GTTGCCAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1277 
                 Encoding 
                 GATAAGTAAGTAGGGATGGTGCCTCAGTTTTTACCC 
               
               
                   
                 Probe 1230 
                 CTGATGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1278 
                 Encoding 
                 GATAAGTAAGTAGGGATGTGTACCTCAGTTTTTACC 
               
               
                   
                 Probe 1231 
                 CCTCATGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1279 
                 Encoding 
                 GATAAGTAAGTAGGGATGGGACTGGTTAGGTTGGGT 
               
               
                   
                 Probe 1232 
                 CACGCCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1280 
                 Encoding 
                 TTAATATGGGTAGTTGGGTCTGTCGAAAACACGGTG 
               
               
                   
                 Probe 1233 
                 AAGAGGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1281 
                 Encoding 
                 TTAATATGGGTAGTTGGGCAGAGTCTGGATGATCAT 
               
               
                   
                 Probe 1234 
                 CCTGAGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1282 
                 Encoding 
                 TTAATATGGGTAGTTGGGTATTCTCGCTTATAAAAGC 
               
               
                   
                 Probe 1235 
                 AGTAATGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1283 
                 Encoding 
                 TTAATATGGGTAGTTGGGTCAAGCTAATAGTCTGAA 
               
               
                   
                 Probe 1236 
                 TGGTTGTCGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1284 
                 Encoding 
                 TTAATATGGGTAGTTGGGCAGTACCCAAAACTGCTA 
               
               
                   
                 Probe 1237 
                 GTATCGTAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1285 
                 Encoding 
                 TTAATATGGGTAGTTGGGATGGACCAGGAAACGTAT 
               
               
                   
                 Probe 1238 
                 TCAGGCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1286 
                 Encoding 
                 TTAATATGGGTAGTTGGGCCCGTCCTACCAGAAAAA 
               
               
                   
                 Probe 1239 
                 TCCAAGACGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1287 
                 Encoding 
                 TTAATATGGGTAGTTGGGTCTGTCGAAAACACGGTG 
               
               
                   
                 Probe 1240 
                 AAGCGGAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1288 
                 Encoding 
                 GATAAGTAAGTAGGGATGTCTGTCGAAAACACGGTG 
               
               
                   
                 Probe 1241 
                 AAGAGGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1289 
                 Encoding 
                 GATAAGTAAGTAGGGATGCAGAGTCTGGATGATCAT 
               
               
                   
                 Probe 1242 
                 CCTGAGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1290 
                 Encoding 
                 GATAAGTAAGTAGGGATGTATTCTCGCTTATAAAAG 
               
               
                   
                 Probe 1243 
                 CAGTAATGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1291 
                 Encoding 
                 GATAAGTAAGTAGGGATGTCAAGCTAATAGTCTGAA 
               
               
                   
                 Probe 1244 
                 TGGTTGTCGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1292 
                 Encoding 
                 GATAAGTAAGTAGGGATGCAGTACCCAAAACTGCTA 
               
               
                   
                 Probe 1245 
                 GTATCGTAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1293 
                 Encoding 
                 GATAAGTAAGTAGGGATGATGGACCAGGAAACGTA 
               
               
                   
                 Probe 1246 
                 TTCAGGCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1294 
                 Encoding 
                 GATAAGTAAGTAGGGATGCCCGTCCTACCAGAAAAA 
               
               
                   
                 Probe 1247 
                 TCCAAGACGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1295 
                 Encoding 
                 GATAAGTAAGTAGGGATGTCTGTCGAAAACACGGTG 
               
               
                   
                 Probe 1248 
                 AAGCGGAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1296 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGAGCGTCAGTACACCGT 
               
               
                   
                 Probe 1249 
                 CCAGGTCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1297 
                 Encoding 
                 GGTAATTGAGTAGAAGGGACGATGCTGCCGGCAGG 
               
               
                   
                 Probe 1250 
                 ATGTGTTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1298 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGTGCAGTCATCGGATCT 
               
               
                   
                 Probe 1251 
                 GCCTAGCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1299 
                 Encoding 
                 GGTAATTGAGTAGAAGGGAGGTCCGAAAAAATTCC 
               
               
                   
                 Probe 1252 
                 GCCCCGGAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1300 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGGACCGAAAAAATTCC 
               
               
                   
                 Probe 1253 
                 GCCCCGGAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1301 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGGTCCGCACCGCATGCG 
               
               
                   
                 Probe 1254 
                 CTTTGGCGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1302 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCGTGCATCCCTCTGTTAA 
               
               
                   
                 Probe 1255 
                 CGCGTAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1303 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTATGAAGTACTCCATCGC 
               
               
                   
                 Probe 1256 
                 TCAGCGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1304 
                 Encoding 
                 GATAAGTAAGTAGGGATGGAGCGTCAGTACACCGTC 
               
               
                   
                 Probe 1257 
                 CAGGTCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1305 
                 Encoding 
                 GATAAGTAAGTAGGGATGACGATGCTGCCGGCAGG 
               
               
                   
                 Probe 1258 
                 ATGTGTTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1306 
                 Encoding 
                 GATAAGTAAGTAGGGATGGTGCAGTCATCGGATCTG 
               
               
                   
                 Probe 1259 
                 CCTAGCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1307 
                 Encoding 
                 GATAAGTAAGTAGGGATGAGGTCCGAAAAAATTCC 
               
               
                   
                 Probe 1260 
                 GCCCCGGAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1308 
                 Encoding 
                 GATAAGTAAGTAGGGATGGGACCGAAAAAATTCCG 
               
               
                   
                 Probe 1261 
                 CCCCGGAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1309 
                 Encoding 
                 GATAAGTAAGTAGGGATGGGTCCGCACCGCATGCGC 
               
               
                   
                 Probe 1262 
                 TTTGGCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1310 
                 Encoding 
                 GATAAGTAAGTAGGGATGCGTGCATCCCTCTGTTAA 
               
               
                   
                 Probe 1263 
                 CGCGTAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1311 
                 Encoding 
                 GATAAGTAAGTAGGGATGTATGAAGTACTCCATCGC 
               
               
                   
                 Probe 1264 
                 TCAGCGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1312 
                 Encoding 
                 GTAATAGATATGAGGGTGGGTCGGCAGCGCAGGATT 
               
               
                   
                 Probe 1265 
                 ATGGCCTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1313 
                 Encoding 
                 GTAATAGATATGAGGGTGTACGCAGCGCAGGATTAT 
               
               
                   
                 Probe 1266 
                 GCGCATTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1314 
                 Encoding 
                 GTAATAGATATGAGGGTGACGCAGCGCAGGATTATG 
               
               
                   
                 Probe 1267 
                 CGGATATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1315 
                 Encoding 
                 GTAATAGATATGAGGGTGGGTCGGCAGCGCAGGATT 
               
               
                   
                 Probe 1268 
                 ATGCCCATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1316 
                 Encoding 
                 GTAATAGATATGAGGGTGGTAGGCAGCGCAGGATTA 
               
               
                   
                 Probe 1269 
                 TGCGGATATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1317 
                 Encoding 
                 GTAATAGATATGAGGGTGGTAGGCAGCGCAGGATTA 
               
               
                   
                 Probe 1270 
                 TGCGCATTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1318 
                 Encoding 
                 GTAATAGATATGAGGGTGGTAGGCAGCGCAGGATTA 
               
               
                   
                 Probe 1271 
                 TGCCCATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1319 
                 Encoding 
                 GTAATAGATATGAGGGTGTACGCAGCGCAGGATTAT 
               
               
                   
                 Probe 1272 
                 GCGGATATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1320 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGGTCGGCAGCGCAGGAT 
               
               
                   
                 Probe 1273 
                 TATGGCCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1321 
                 Encoding 
                 AGTATTATTAGGGTGAGGTACGCAGCGCAGGATTAT 
               
               
                   
                 Probe 1274 
                 GCGCATGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1322 
                 Encoding 
                 AGTATTATTAGGGTGAGGACGCAGCGCAGGATTATG 
               
               
                   
                 Probe 1275 
                 CGGATAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1323 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGGTCGGCAGCGCAGGAT 
               
               
                   
                 Probe 1276 
                 TATGCCCAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1324 
                 Encoding 
                 AGTATTATTAGGGTGAGGGTAGGCAGCGCAGGATTA 
               
               
                   
                 Probe 1277 
                 TGCGGATAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1325 
                 Encoding 
                 AGTATTATTAGGGTGAGGGTAGGCAGCGCAGGATTA 
               
               
                   
                 Probe 1278 
                 TGCGCATGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1326 
                 Encoding 
                 AGTATTATTAGGGTGAGGGTAGGCAGCGCAGGATTA 
               
               
                   
                 Probe 1279 
                 TGCCCAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1327 
                 Encoding 
                 AGTATTATTAGGGTGAGGTACGCAGCGCAGGATTAT 
               
               
                   
                 Probe 1280 
                 GCGGATAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1328 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGTACACCCAGTATCAAC 
               
               
                   
                 Probe 1281 
                 TGCTTAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1329 
                 Encoding 
                 GGAATTTAGTGAGAAGGGACCGGTTCAGACTCTCGT 
               
               
                   
                 Probe 1282 
                 CCAAACGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1330 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCAGCACATCATTCAGTTG 
               
               
                   
                 Probe 1283 
                 CAAAAGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1331 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTCTCTTTCGGGATTAGCA 
               
               
                   
                 Probe 1284 
                 TCACCAGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1332 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGGCGGAAGAACTATG 
               
               
                   
                 Probe 1285 
                 CCATCCCCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1333 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGTCGGAAGAACTATGC 
               
               
                   
                 Probe 1286 
                 CATCCCCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1334 
                 Encoding 
                 GGAATTTAGTGAGAAGGGACTGAAGTTCTTTAATAG 
               
               
                   
                 Probe 1287 
                 TTCTACCAACGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1335 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGAGTTCTTTAATAGTT 
               
               
                   
                 Probe 1288 
                 CTACCATGGCCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1336 
                 Encoding 
                 AGTATTATTAGGGTGAGGAGTACACCCAGTATCAAC 
               
               
                   
                 Probe 1289 
                 TGCTTAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1337 
                 Encoding 
                 AGTATTATTAGGGTGAGGACCGGTTCAGACTCTCGT 
               
               
                   
                 Probe 1290 
                 CCAAACGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1338 
                 Encoding 
                 AGTATTATTAGGGTGAGGCAGCACATCATTCAGTTG 
               
               
                   
                 Probe 1291 
                 CAAAAGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1339 
                 Encoding 
                 AGTATTATTAGGGTGAGGTCTCTTTCGGGATTAGCAT 
               
               
                   
                 Probe 1292 
                 CACCAGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1340 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGGGCGGAAGAACTATGC 
               
               
                   
                 Probe 1293 
                 CATCCCCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1341 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGGTCGGAAGAACTATGC 
               
               
                   
                 Probe 1294 
                 CATCCCCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1342 
                 Encoding 
                 AGTATTATTAGGGTGAGGACTGAAGTTCTTTAATAG 
               
               
                   
                 Probe 1295 
                 TTCTACCAACGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1343 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGGAGTTCTTTAATAGTTC 
               
               
                   
                 Probe 1296 
                 TACCATGGCCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1344 
                 Encoding 
                 ATAAGATAGTGAGATGGGATCGGAGCTTTCTTGCAG 
               
               
                   
                 Probe 1297 
                 GGTAGGCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1345 
                 Encoding 
                 ATAAGATAGTGAGATGGGATCGGAGCTTTCTTGCAG 
               
               
                   
                 Probe 1298 
                 GGTTGGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1346 
                 Encoding 
                 ATAAGATAGTGAGATGGGTCTTCACATTCAACTTAT 
               
               
                   
                 Probe 1299 
                 CCTCCGCGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1347 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGCAGTCCCATTAGAGTG 
               
               
                   
                 Probe 1300 
                 CTCAACGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1348 
                 Encoding 
                 ATAAGATAGTGAGATGGGAATCTATTGACTTCGGGT 
               
               
                   
                 Probe 1301 
                 GTTTGGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1349 
                 Encoding 
                 ATAAGATAGTGAGATGGGTCGGAGCTTTCTTGCAGG 
               
               
                   
                 Probe 1302 
                 GTAGGCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1350 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGCAGTCCCATTAGAGTG 
               
               
                   
                 Probe 1303 
                 CTCTACGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1351 
                 Encoding 
                 ATAAGATAGTGAGATGGGATCTTCACATTCAACTTA 
               
               
                   
                 Probe 1304 
                 TCCTCCGCGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1352 
                 Encoding 
                 AGTATTATTAGGGTGAGGATCGGAGCTTTCTTGCAG 
               
               
                   
                 Probe 1305 
                 GGTAGGCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1353 
                 Encoding 
                 AGTATTATTAGGGTGAGGATCGGAGCTTTCTTGCAG 
               
               
                   
                 Probe 1306 
                 GGTTGGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1354 
                 Encoding 
                 AGTATTATTAGGGTGAGGTCTTCACATTCAACTTATC 
               
               
                   
                 Probe 1307 
                 CTCCGCGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1355 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGCAGTCCCATTAGAGTG 
               
               
                   
                 Probe 1308 
                 CTCAACGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1356 
                 Encoding 
                 AGTATTATTAGGGTGAGGAATCTATTGACTTCGGGT 
               
               
                   
                 Probe 1309 
                 GTTTGGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1357 
                 Encoding 
                 AGTATTATTAGGGTGAGGTCGGAGCTTTCTTGCAGG 
               
               
                   
                 Probe 1310 
                 GTAGGCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1358 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGCAGTCCCATTAGAGTG 
               
               
                   
                 Probe 1311 
                 CTCTACGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1359 
                 Encoding 
                 AGTATTATTAGGGTGAGGATCTTCACATTCAACTTAT 
               
               
                   
                 Probe 1312 
                 CCTCCGCGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1360 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGAAGTACAAACAGGATG 
               
               
                   
                 Probe 1313 
                 TCCCATCCGATGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1361 
                 Encoding 
                 TTAATATGGGTAGTTGGGTAGTGGTACAAACAGGAT 
               
               
                   
                 Probe 1314 
                 GTCCGTAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1362 
                 Encoding 
                 TTAATATGGGTAGTTGGGTAGTGGTACAAACAGGAT 
               
               
                   
                 Probe 1315 
                 GTCGGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1363 
                 Encoding 
                 TTAATATGGGTAGTTGGGAAATACAAACAGGATGTC 
               
               
                   
                 Probe 1316 
                 CCATCCGATGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1364 
                 Encoding 
                 TTAATATGGGTAGTTGGGAACACAAACAGGATGTCC 
               
               
                   
                 Probe 1317 
                 CATCCGATGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1365 
                 Encoding 
                 TTAATATGGGTAGTTGGGCTAATCTTTGGTACAAAC 
               
               
                   
                 Probe 1318 
                 AGGAACAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1366 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGAGGAGTTGCAGTTTTG 
               
               
                   
                 Probe 1319 
                 AGTGGCTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1367 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGAAGAGTTGCAGTTTTG 
               
               
                   
                 Probe 1320 
                 AGTGGCTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1368 
                 Encoding 
                 AGTATTATTAGGGTGAGGGAAGTACAAACAGGATGT 
               
               
                   
                 Probe 1321 
                 CCCATCCGATGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1369 
                 Encoding 
                 AGTATTATTAGGGTGAGGTAGTGGTACAAACAGGAT 
               
               
                   
                 Probe 1322 
                 GTCCGTAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1370 
                 Encoding 
                 AGTATTATTAGGGTGAGGTAGTGGTACAAACAGGAT 
               
               
                   
                 Probe 1323 
                 GTCGGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1371 
                 Encoding 
                 AGTATTATTAGGGTGAGGAAATACAAACAGGATGTC 
               
               
                   
                 Probe 1324 
                 CCATCCGATGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1372 
                 Encoding 
                 AGTATTATTAGGGTGAGGAACACAAACAGGATGTCC 
               
               
                   
                 Probe 1325 
                 CATCCGATGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1373 
                 Encoding 
                 AGTATTATTAGGGTGAGGCTAATCTTTGGTACAAAC 
               
               
                   
                 Probe 1326 
                 AGGAACAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1374 
                 Encoding 
                 AGTATTATTAGGGTGAGGGAGGAGTTGCAGTTTTGA 
               
               
                   
                 Probe 1327 
                 GTGGCTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1375 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGAAGAGTTGCAGTTTTG 
               
               
                   
                 Probe 1328 
                 AGTGGCTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1376 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGAACTTCACTCAAGAA 
               
               
                   
                 Probe 1329 
                 CAGCTCAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1377 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCGATCTCTAAGCTCTTCT 
               
               
                   
                 Probe 1330 
                 TGGGATGTGTTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1378 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCAACTCTGCTTCGCAGCT 
               
               
                   
                 Probe 1331 
                 TTGGAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1379 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTTTGGTCAGCCCCCCCCA 
               
               
                   
                 Probe 1332 
                 CACGATGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1380 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGCGGCGCCCTCCTAAAA 
               
               
                   
                 Probe 1333 
                 GGTATCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1381 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAATGTCCCTTAAGACAGA 
               
               
                   
                 Probe 1334 
                 GGTAATGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1382 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCCGTTCTACCTCTCAGTA 
               
               
                   
                 Probe 1335 
                 CGGGATGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1383 
                 Encoding 
                 GGAATTTAGTGAGAAGGGAGGCACTAACTTGAGAG 
               
               
                   
                 Probe 1336 
                 AGCATCGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1384 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGGAACTTCACTCAAGAAC 
               
               
                   
                 Probe 1337 
                 AGCTCAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1385 
                 Encoding 
                 ATGTATTAAGAGGAGGGACGATCTCTAAGCTCTTCT 
               
               
                   
                 Probe 1338 
                 TGGGATGTGTTGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1386 
                 Encoding 
                 ATGTATTAAGAGGAGGGACAACTCTGCTTCGCAGCT 
               
               
                   
                 Probe 1339 
                 TTGGAAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1387 
                 Encoding 
                 ATGTATTAAGAGGAGGGATTTGGTCAGCCCCCCCCA 
               
               
                   
                 Probe 1340 
                 CACGATGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1388 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAGCGGCGCCCTCCTAAAA 
               
               
                   
                 Probe 1341 
                 GGTATCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1389 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAATGTCCCTTAAGACAGA 
               
               
                   
                 Probe 1342 
                 GGTAATGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1390 
                 Encoding 
                 ATGTATTAAGAGGAGGGACCGTTCTACCTCTCAGTA 
               
               
                   
                 Probe 1343 
                 CGGGATGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1391 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAGGCACTAACTTGAGAG 
               
               
                   
                 Probe 1344 
                 AGCATCGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1392 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGCGCATTGCTGGGTAAG 
               
               
                   
                 Probe 1345 
                 AGTAAGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1393 
                 Encoding 
                 ATAAGATAGTGAGATGGGTCACTAACTTAATATTGG 
               
               
                   
                 Probe 1346 
                 CAACTAGTATAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1394 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGAAACCGTATTAGCACA 
               
               
                   
                 Probe 1347 
                 AATTTCAGAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1395 
                 Encoding 
                 ATAAGATAGTGAGATGGGCAGATACCGTATTAGCAC 
               
               
                   
                 Probe 1348 
                 AAATTTGAGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1396 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGCAGCTTCGGCGCAGAA 
               
               
                   
                 Probe 1349 
                 GGAGAGCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1397 
                 Encoding 
                 ATAAGATAGTGAGATGGGAATTTCGGCGCAGAAGG 
               
               
                   
                 Probe 1350 
                 AGTCCTAGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1398 
                 Encoding 
                 ATAAGATAGTGAGATGGGTGCGCATTGCTGGGTAAG 
               
               
                   
                 Probe 1351 
                 AGTTAGGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1399 
                 Encoding 
                 ATAAGATAGTGAGATGGGATCATTCCACTTTCCTCT 
               
               
                   
                 Probe 1352 
                 ACTGGTGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1400 
                 Encoding 
                 ATGTATTAAGAGGAGGGATGCGCATTGCTGGGTAAG 
               
               
                   
                 Probe 1353 
                 AGTAAGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1401 
                 Encoding 
                 ATGTATTAAGAGGAGGGATCACTAACTTAATATTGG 
               
               
                   
                 Probe 1354 
                 CAACTAGTATAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1402 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGAAACCGTATTAGCACA 
               
               
                   
                 Probe 1355 
                 AATTTCAGAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1403 
                 Encoding 
                 ATGTATTAAGAGGAGGGACAGATACCGTATTAGCAC 
               
               
                   
                 Probe 1356 
                 AAATTTGAGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1404 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGCAGCTTCGGCGCAGAA 
               
               
                   
                 Probe 1357 
                 GGAGAGCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1405 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAATTTCGGCGCAGAAGG 
               
               
                   
                 Probe 1358 
                 AGTCCTAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1406 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGCGCATTGCTGGGTAAGA 
               
               
                   
                 Probe 1359 
                 GTTAGGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1407 
                 Encoding 
                 ATGTATTAAGAGGAGGGAATCATTCCACTTTCCTCT 
               
               
                   
                 Probe 1360 
                 ACTGGTGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1408 
                 Encoding 
                 TTAATATGGGTAGTTGGGATCTCAATTTCTTGACGTT 
               
               
                   
                 Probe 1361 
                 ATCCGAGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1409 
                 Encoding 
                 TTAATATGGGTAGTTGGGCAATTATGCGGTTCCTGG 
               
               
                   
                 Probe 1362 
                 GTTGTCGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1410 
                 Encoding 
                 TTAATATGGGTAGTTGGGCTTAACTCCGCTTTACACG 
               
               
                   
                 Probe 1363 
                 GCCACAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1411 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGTTAGCGCTCATCGTTTA 
               
               
                   
                 Probe 1364 
                 CACGCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1412 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGCACTTCCTTCTTCCCT 
               
               
                   
                 Probe 1365 
                 GCACTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1413 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGCAATTCCTTGCCGAC 
               
               
                   
                 Probe 1366 
                 ACCATCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1414 
                 Encoding 
                 TTAATATGGGTAGTTGGGCAACTTCACTCTGTTTCAG 
               
               
                   
                 Probe 1367 
                 CCTAAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1415 
                 Encoding 
                 TTAATATGGGTAGTTGGGAACGATAAATCTTTTCTCT 
               
               
                   
                 Probe 1368 
                 CGCCACGTACGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1416 
                 Encoding 
                 ATGTATTAAGAGGAGGGAATCTCAATTTCTTGACGT 
               
               
                   
                 Probe 1369 
                 TATCCGAGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1417 
                 Encoding 
                 ATGTATTAAGAGGAGGGACAATTATGCGGTTCCTGG 
               
               
                   
                 Probe 1370 
                 GTTGTCGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1418 
                 Encoding 
                 ATGTATTAAGAGGAGGGACTTAACTCCGCTTTACAC 
               
               
                   
                 Probe 1371 
                 GGCCACAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1419 
                 Encoding 
                 ATGTATTAAGAGGAGGGATGTTAGCGCTCATCGTTT 
               
               
                   
                 Probe 1372 
                 ACACGCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1420 
                 Encoding 
                 ATGTATTAAGAGGAGGGATGGCACTTCCTTCTTCCCT 
               
               
                   
                 Probe 1373 
                 GCACTGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1421 
                 Encoding 
                 ATGTATTAAGAGGAGGGATGGCAATTCCTTGCCGAC 
               
               
                   
                 Probe 1374 
                 ACCATCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1422 
                 Encoding 
                 ATGTATTAAGAGGAGGGACAACTTCACTCTGTTTCA 
               
               
                   
                 Probe 1375 
                 GCCTAAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1423 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAACGATAAATCTTTTCTC 
               
               
                   
                 Probe 1376 
                 TCGCCACGTACGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1424 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCGGTAAATCTTTTCACAC 
               
               
                   
                 Probe 1377 
                 CATGCGTAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1425 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGCCCCGAAGGATTGTTT 
               
               
                   
                 Probe 1378 
                 TACTACGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1426 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGACCCGTAGGAAAAGA 
               
               
                   
                 Probe 1379 
                 CACATTACACAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1427 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCGGACAGCTCTGCTTCCC 
               
               
                   
                 Probe 1380 
                 TTTCAAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1428 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGGAGAGTTATCCTCGGC 
               
               
                   
                 Probe 1381 
                 TGTCGGAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1429 
                 Encoding 
                 GGTAATTGAGTAGAAGGGACGATAAATCTTTTCACA 
               
               
                   
                 Probe 1382 
                 CCATGCGTAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1430 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGCCCCGAAGGATTGTTT 
               
               
                   
                 Probe 1383 
                 TACAACGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1431 
                 Encoding 
                 GGTAATTGAGTAGAAGGGACACGTAGGAAAAGACA 
               
               
                   
                 Probe 1384 
                 CATTACACAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1432 
                 Encoding 
                 ATGTATTAAGAGGAGGGACGGTAAATCTTTTCACAC 
               
               
                   
                 Probe 1385 
                 CATGCGTAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1433 
                 Encoding 
                 ATGTATTAAGAGGAGGGATGCCCCGAAGGATTGTTT 
               
               
                   
                 Probe 1386 
                 TACTACGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1434 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGACCCGTAGGAAAAGAC 
               
               
                   
                 Probe 1387 
                 ACATTACACAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1435 
                 Encoding 
                 ATGTATTAAGAGGAGGGACGGACAGCTCTGCTTCCC 
               
               
                   
                 Probe 1388 
                 TTTCAAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1436 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGGAGAGTTATCCTCGGCT 
               
               
                   
                 Probe 1389 
                 GTCGGAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1437 
                 Encoding 
                 ATGTATTAAGAGGAGGGAACGATAAATCTTTTCACA 
               
               
                   
                 Probe 1390 
                 CCATGCGTAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1438 
                 Encoding 
                 ATGTATTAAGAGGAGGGATGCCCCGAAGGATTGTTT 
               
               
                   
                 Probe 1391 
                 TACAACGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1439 
                 Encoding 
                 ATGTATTAAGAGGAGGGAACACGTAGGAAAAGACA 
               
               
                   
                 Probe 1392 
                 CATTACACAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1440 
                 Encoding 
                 GTAATAGATATGAGGGTGTTTCATGCGACTTAGTTG 
               
               
                   
                 Probe 1393 
                 CATATATGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1441 
                 Encoding 
                 GTAATAGATATGAGGGTGTCGTGCGACTTAGTTGCA 
               
               
                   
                 Probe 1394 
                 TTAACGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1442 
                 Encoding 
                 GTAATAGATATGAGGGTGGCGTTTTGCCTCTCTTTGT 
               
               
                   
                 Probe 1395 
                 TGTGGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1443 
                 Encoding 
                 GTAATAGATATGAGGGTGTTCATGCGACTTAGTTGC 
               
               
                   
                 Probe 1396 
                 ATTTACTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1444 
                 Encoding 
                 GTAATAGATATGAGGGTGAGCGTTTTGCCTCTCTTTG 
               
               
                   
                 Probe 1397 
                 TTGTGGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1445 
                 Encoding 
                 GTAATAGATATGAGGGTGAGCGTTTTGCCTCTCTTTG 
               
               
                   
                 Probe 1398 
                 TTCTGTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1446 
                 Encoding 
                 GTAATAGATATGAGGGTGGAGGGTTTTGCCTCTCTTT 
               
               
                   
                 Probe 1399 
                 GTACTTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1447 
                 Encoding 
                 GTAATAGATATGAGGGTGGTTCCATGCGACTTAGTT 
               
               
                   
                 Probe 1400 
                 GCAAATTGGGAGGGTAATAGATAT 
               
               
                   
               
               
                 1448 
                 Encoding 
                 TAGAATTAGAGAGATGGGTTTCATGCGACTTAGTTG 
               
               
                   
                 Probe 1401 
                 CATATAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1449 
                 Encoding 
                 TAGAATTAGAGAGATGGGTCGTGCGACTTAGTTGCA 
               
               
                   
                 Probe 1402 
                 TTAACGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1450 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGCGTTTTGCCTCTCTTTG 
               
               
                   
                 Probe 1403 
                 TTGTGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1451 
                 Encoding 
                 TAGAATTAGAGAGATGGGTTCATGCGACTTAGTTGC 
               
               
                   
                 Probe 1404 
                 ATTTACGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1452 
                 Encoding 
                 TAGAATTAGAGAGATGGGAGCGTTTTGCCTCTCTTT 
               
               
                   
                 Probe 1405 
                 GTTGTGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1453 
                 Encoding 
                 TAGAATTAGAGAGATGGGAGCGTTTTGCCTCTCTTT 
               
               
                   
                 Probe 1406 
                 GTTCTGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1454 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGAGGGTTTTGCCTCTCT 
               
               
                   
                 Probe 1407 
                 TTGTACTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1455 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGTTCCATGCGACTTAGT 
               
               
                   
                 Probe 1408 
                 TGCAAATGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1456 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGTAGAATAGGAATCA 
               
               
                   
                 Probe 1409 
                 CTAGGTTTCTAGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1457 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCGGTAGAATAGGAATCA 
               
               
                   
                 Probe 1410 
                 CTAGGTTTCTAGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1458 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGCACTAGAATAGGAA 
               
               
                   
                 Probe 1411 
                 TCACTAGGTAAGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1459 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGCACTAGAATAGGAA 
               
               
                   
                 Probe 1412 
                 TCACTAGGAAAGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1460 
                 Encoding 
                 GGAATTTAGTGAGAAGGGCAGCCACTAGAATAGGA 
               
               
                   
                 Probe 1413 
                 ATCACTTCCGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1461 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGCAGCCACTAGAATAG 
               
               
                   
                 Probe 1414 
                 GAATCAGATGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1462 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGCGCTAGAATAGGAATC 
               
               
                   
                 Probe 1415 
                 ACTAGGTAAGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1463 
                 Encoding 
                 GGAATTTAGTGAGAAGGGTGGTAGAATAGGAATCA 
               
               
                   
                 Probe 1416 
                 CTAGGTTTCAAGGTGGGTGTTGGAATTTAGTG 
               
               
                   
               
               
                 1464 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGTAGAATAGGAATCA 
               
               
                   
                 Probe 1417 
                 CTAGGTTTCTAGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1465 
                 Encoding 
                 TAGAATTAGAGAGATGGGCGGTAGAATAGGAATCA 
               
               
                   
                 Probe 1418 
                 CTAGGTTTCTAGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1466 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGCACTAGAATAGGAA 
               
               
                   
                 Probe 1419 
                 TCACTAGGTAAGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1467 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGCACTAGAATAGGAA 
               
               
                   
                 Probe 1420 
                 TCACTAGGAAAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1468 
                 Encoding 
                 TAGAATTAGAGAGATGGGCAGCCACTAGAATAGGA 
               
               
                   
                 Probe 1421 
                 ATCACTTCCGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1469 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGCAGCCACTAGAATAG 
               
               
                   
                 Probe 1422 
                 GAATCAGATGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1470 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGCGCTAGAATAGGAATC 
               
               
                   
                 Probe 1423 
                 ACTAGGTAAGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1471 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGTAGAATAGGAATCA 
               
               
                   
                 Probe 1424 
                 CTAGGTTTCAAGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1472 
                 Encoding 
                 ATAAGATAGTGAGATGGGAGGTAGGAAGGGCGACA 
               
               
                   
                 Probe 1425 
                 TTACAGCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1473 
                 Encoding 
                 ATAAGATAGTGAGATGGGTCACCAAAGCAGTCCACA 
               
               
                   
                 Probe 1426 
                 GGTTCTCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1474 
                 Encoding 
                 ATAAGATAGTGAGATGGGAGTCAAATCACTTCTCCT 
               
               
                   
                 Probe 1427 
                 CCCCTTGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1475 
                 Encoding 
                 ATAAGATAGTGAGATGGGCGACTCCGGTTAGGGTTG 
               
               
                   
                 Probe 1428 
                 GGTGTGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1476 
                 Encoding 
                 ATAAGATAGTGAGATGGGCACCATCCTTGATGCTGG 
               
               
                   
                 Probe 1429 
                 CTAGACGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1477 
                 Encoding 
                 ATAAGATAGTGAGATGGGCAACAAAGCAGTCCACA 
               
               
                   
                 Probe 1430 
                 GGTTCTCGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1478 
                 Encoding 
                 ATAAGATAGTGAGATGGGAGGCGCTCAGTCAAATCA 
               
               
                   
                 Probe 1431 
                 CTTGAGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1479 
                 Encoding 
                 ATAAGATAGTGAGATGGGCAGGTAGGAAGGGCGAC 
               
               
                   
                 Probe 1432 
                 ATTAGAGGTGGGTGATAAGATAGTG 
               
               
                   
               
               
                 1480 
                 Encoding 
                 TAGAATTAGAGAGATGGGAGGTAGGAAGGGCGACA 
               
               
                   
                 Probe 1433 
                 TTACAGCGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1481 
                 Encoding 
                 TAGAATTAGAGAGATGGGTCACCAAAGCAGTCCACA 
               
               
                   
                 Probe 1434 
                 GGTTCTCGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1482 
                 Encoding 
                 TAGAATTAGAGAGATGGGAGTCAAATCACTTCTCCT 
               
               
                   
                 Probe 1435 
                 CCCCTTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1483 
                 Encoding 
                 TAGAATTAGAGAGATGGGCGACTCCGGTTAGGGTTG 
               
               
                   
                 Probe 1436 
                 GGTGTGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1484 
                 Encoding 
                 TAGAATTAGAGAGATGGGCACCATCCTTGATGCTGG 
               
               
                   
                 Probe 1437 
                 CTAGACGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1485 
                 Encoding 
                 TAGAATTAGAGAGATGGGCAACAAAGCAGTCCACA 
               
               
                   
                 Probe 1438 
                 GGTTCTCGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1486 
                 Encoding 
                 TAGAATTAGAGAGATGGGAGGCGCTCAGTCAAATCA 
               
               
                   
                 Probe 1439 
                 CTTGAGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1487 
                 Encoding 
                 TAGAATTAGAGAGATGGGCAGGTAGGAAGGGCGAC 
               
               
                   
                 Probe 1440 
                 ATTAGAGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1488 
                 Encoding 
                 TTAATATGGGTAGTTGGGCAGGCGACTTCGTGGTCT 
               
               
                   
                 Probe 1441 
                 TATGGCCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1489 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGCGGAGCTTTTACCCCA 
               
               
                   
                 Probe 1442 
                 AAGTCTACGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1490 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGCGGAGCTTTTACCCC 
               
               
                   
                 Probe 1443 
                 AAAGTGTAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1491 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGAAGTCATGCGACTTC 
               
               
                   
                 Probe 1444 
                 GTGGAGAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1492 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGAAAGTCATGCGACTT 
               
               
                   
                 Probe 1445 
                 CGTGCAGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1493 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGCGGAGCTTTTACCCCA 
               
               
                   
                 Probe 1446 
                 AAGTGTAGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1494 
                 Encoding 
                 TTAATATGGGTAGTTGGGAGTCGACTTCGTGGTCTTA 
               
               
                   
                 Probe 1447 
                 TGGCCGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1495 
                 Encoding 
                 TTAATATGGGTAGTTGGGTGGCGGAGCTTTTACCCC 
               
               
                   
                 Probe 1448 
                 AAAGAGTGGGTGTGTTAATATGGGT 
               
               
                   
               
               
                 1496 
                 Encoding 
                 TAGAATTAGAGAGATGGGCAGGCGACTTCGTGGTCT 
               
               
                   
                 Probe 1449 
                 TATGGCCGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1497 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGCGGAGCTTTTACCCCA 
               
               
                   
                 Probe 1450 
                 AAGTCTACGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1498 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGCGGAGCTTTTACCCC 
               
               
                   
                 Probe 1451 
                 AAAGTGTAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1499 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGAAGTCATGCGACTTC 
               
               
                   
                 Probe 1452 
                 GTGGAGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1500 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGAAAGTCATGCGACTT 
               
               
                   
                 Probe 1453 
                 CGTGCAGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1501 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGCGGAGCTTTTACCCCA 
               
               
                   
                 Probe 1454 
                 AAGTGTAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1502 
                 Encoding 
                 TAGAATTAGAGAGATGGGAGTCGACTTCGTGGTCTT 
               
               
                   
                 Probe 1455 
                 ATGGCCGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1503 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGCGGAGCTTTTACCCC 
               
               
                   
                 Probe 1456 
                 AAAGAGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1504 
                 Encoding 
                 GGTAATTGAGTAGAAGGGACAGTTACAGTCTAGCAA 
               
               
                   
                 Probe 1457 
                 CCCCGGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1505 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGCACACTAGGAATTCCG 
               
               
                   
                 Probe 1458 
                 GTTGAGGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1506 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCAAAGTAGTAGTTCCAAG 
               
               
                   
                 Probe 1459 
                 GTTGTCGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1507 
                 Encoding 
                 GGTAATTGAGTAGAAGGGACAGTTACAGTCTAGCAA 
               
               
                   
                 Probe 1460 
                 CCCGGGAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1508 
                 Encoding 
                 GGTAATTGAGTAGAAGGGAACCCTTGGAGTTACGCT 
               
               
                   
                 Probe 1461 
                 ACTTTGTGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1509 
                 Encoding 
                 GGTAATTGAGTAGAAGGGCAGTTACAGTCTAGCAAC 
               
               
                   
                 Probe 1462 
                 CCGGGAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1510 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGGTGTTGAGCCTTGGAG 
               
               
                   
                 Probe 1463 
                 TTACCGAGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1511 
                 Encoding 
                 GGTAATTGAGTAGAAGGGTGTTGTTTTAGTAGTAGT 
               
               
                   
                 Probe 1464 
                 TCCAAGGAACGGGATGAGGTAATTGAGT 
               
               
                   
               
               
                 1512 
                 Encoding 
                 TAGAATTAGAGAGATGGGACAGTTACAGTCTAGCAA 
               
               
                   
                 Probe 1465 
                 CCCCGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1513 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGCACACTAGGAATTCCG 
               
               
                   
                 Probe 1466 
                 GTTGAGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1514 
                 Encoding 
                 TAGAATTAGAGAGATGGGCAAAGTAGTAGTTCCAAG 
               
               
                   
                 Probe 1467 
                 GTTGTCGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1515 
                 Encoding 
                 TAGAATTAGAGAGATGGGACAGTTACAGTCTAGCAA 
               
               
                   
                 Probe 1468 
                 CCCGGGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1516 
                 Encoding 
                 TAGAATTAGAGAGATGGGAACCCTTGGAGTTACGCT 
               
               
                   
                 Probe 1469 
                 ACTTTGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1517 
                 Encoding 
                 TAGAATTAGAGAGATGGGCAGTTACAGTCTAGCAAC 
               
               
                   
                 Probe 1470 
                 CCGGGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1518 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGTGTTGAGCCTTGGAG 
               
               
                   
                 Probe 1471 
                 TTACCGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1519 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGTTGTTTTAGTAGTAGT 
               
               
                   
                 Probe 1472 
                 TCCAAGGAACGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1520 
                 Encoding 
                 TGTATAGGATTAGAAGGGCGACTTGATAGGTACAGT 
               
               
                   
                 Probe 1473 
                 CTTTTTTGAAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1521 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGTGACTAGTTAATCAGG 
               
               
                   
                 Probe 1474 
                 CGCATCCGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1522 
                 Encoding 
                 TGTATAGGATTAGAAGGGTACCCGAAATACTATCTA 
               
               
                   
                 Probe 1475 
                 CTTTCATACTAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1523 
                 Encoding 
                 TGTATAGGATTAGAAGGGAAGTTCTCTCTGTAATAG 
               
               
                   
                 Probe 1476 
                 CCATTCATGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1524 
                 Encoding 
                 TGTATAGGATTAGAAGGGTCGTCTTGATAGGTACAG 
               
               
                   
                 Probe 1477 
                 TCTTTTTTGAAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1525 
                 Encoding 
                 TGTATAGGATTAGAAGGGCAAGCTTGACCTTGCGGT 
               
               
                   
                 Probe 1478 
                 TTCCGAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1526 
                 Encoding 
                 TGTATAGGATTAGAAGGGCGCAGGCCATCTATTAGT 
               
               
                   
                 Probe 1479 
                 GGAAACGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1527 
                 Encoding 
                 TGTATAGGATTAGAAGGGCCGAAGGCCATCTATTAG 
               
               
                   
                 Probe 1480 
                 TGGAAACGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1528 
                 Encoding 
                 TATAGTTATGGAGAAGGGCGACTTGATAGGTACAGT 
               
               
                   
                 Probe 1481 
                 CTTTTTTGAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1529 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGTGACTAGTTAATCAGG 
               
               
                   
                 Probe 1482 
                 CGCATCCGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1530 
                 Encoding 
                 TATAGTTATGGAGAAGGGTACCCGAAATACTATCTA 
               
               
                   
                 Probe 1483 
                 CTTTCATACTAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1531 
                 Encoding 
                 TATAGTTATGGAGAAGGGAAGTTCTCTCTGTAATAG 
               
               
                   
                 Probe 1484 
                 CCATTCATGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1532 
                 Encoding 
                 TATAGTTATGGAGAAGGGTCGTCTTGATAGGTACAG 
               
               
                   
                 Probe 1485 
                 TCTTTTTTGAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1533 
                 Encoding 
                 TATAGTTATGGAGAAGGGCAAGCTTGACCTTGCGGT 
               
               
                   
                 Probe 1486 
                 TTCCGAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1534 
                 Encoding 
                 TATAGTTATGGAGAAGGGCGCAGGCCATCTATTAGT 
               
               
                   
                 Probe 1487 
                 GGAAACGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1535 
                 Encoding 
                 TATAGTTATGGAGAAGGGCCGAAGGCCATCTATTAG 
               
               
                   
                 Probe 1488 
                 TGGAAACGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1536 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTAAACATCTGACTTGACA 
               
               
                   
                 Probe 1489 
                 GACCCGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1537 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGGACTCTACAAGACTCT 
               
               
                   
                 Probe 1490 
                 AGCCTCGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1538 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGATACCCTCTGTCAGGCAG 
               
               
                   
                 Probe 1491 
                 ATCAGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1539 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTAGCCTCTGTCAGGCAGA 
               
               
                   
                 Probe 1492 
                 TCCGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1540 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGCCAATCTGACAGCGAGA 
               
               
                   
                 Probe 1493 
                 GGCCGCTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1541 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCAATTGCTGAGGTTAT 
               
               
                   
                 Probe 1494 
                 TAACCAGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1542 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTCGCTCCTCAAGGGAAC 
               
               
                   
                 Probe 1495 
                 AACCAGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1543 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTCCTCTCTGCCAAATTCC 
               
               
                   
                 Probe 1496 
                 GTGCTAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1544 
                 Encoding 
                 TATAGTTATGGAGAAGGGTAAACATCTGACTTGACA 
               
               
                   
                 Probe 1497 
                 GACCCGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1545 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGGACTCTACAAGACTCT 
               
               
                   
                 Probe 1498 
                 AGCCTCGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1546 
                 Encoding 
                 TATAGTTATGGAGAAGGGATACCCTCTGTCAGGCAG 
               
               
                   
                 Probe 1499 
                 ATCAGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1547 
                 Encoding 
                 TATAGTTATGGAGAAGGGTAGCCTCTGTCAGGCAGA 
               
               
                   
                 Probe 1500 
                 TCCGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1548 
                 Encoding 
                 TATAGTTATGGAGAAGGGCCAATCTGACAGCGAGAG 
               
               
                   
                 Probe 1501 
                 GCCGCTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1549 
                 Encoding 
                 TATAGTTATGGAGAAGGGTGCAATTGCTGAGGTTAT 
               
               
                   
                 Probe 1502 
                 TAACCAGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1550 
                 Encoding 
                 TATAGTTATGGAGAAGGGTCGCTCCTCAAGGGAACA 
               
               
                   
                 Probe 1503 
                 ACCAGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1551 
                 Encoding 
                 TATAGTTATGGAGAAGGGTCCTCTCTGCCAAATTCC 
               
               
                   
                 Probe 1504 
                 GTGCTAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1552 
                 Encoding 
                 GGATAGAGTATAGTTGGGTTCCTGACGGCTTTACCC 
               
               
                   
                 Probe 1505 
                 ATCATAGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1553 
                 Encoding 
                 GGATAGAGTATAGTTGGGTCCTGACGGCTTTACCCA 
               
               
                   
                 Probe 1506 
                 TCATAGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1554 
                 Encoding 
                 GGATAGAGTATAGTTGGGAGGAAGGCCTGACGGCTT 
               
               
                   
                 Probe 1507 
                 TACGGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1555 
                 Encoding 
                 GGATAGAGTATAGTTGGGTTCCTGACGGCTTTACCC 
               
               
                   
                 Probe 1508 
                 ATCAAAGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1556 
                 Encoding 
                 GGATAGAGTATAGTTGGGCCGGACGGCTTTACCCAT 
               
               
                   
                 Probe 1509 
                 CATAGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1557 
                 Encoding 
                 GGATAGAGTATAGTTGGGCAGGAAGGCCTGACGGCT 
               
               
                   
                 Probe 1510 
                 TTAAGGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1558 
                 Encoding 
                 GGATAGAGTATAGTTGGGTTCCTGACGGCTTTACCC 
               
               
                   
                 Probe 1511 
                 ATCTAAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1559 
                 Encoding 
                 GGATAGAGTATAGTTGGGTCCTGACGGCTTTACCCA 
               
               
                   
                 Probe 1512 
                 TCAAAGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1560 
                 Encoding 
                 TATAGTTATGGAGAAGGGTTCCTGACGGCTTTACCC 
               
               
                   
                 Probe 1513 
                 ATCATAGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1561 
                 Encoding 
                 TATAGTTATGGAGAAGGGTCCTGACGGCTTTACCCA 
               
               
                   
                 Probe 1514 
                 TCATAGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1562 
                 Encoding 
                 TATAGTTATGGAGAAGGGAGGAAGGCCTGACGGCTT 
               
               
                   
                 Probe 1515 
                 TACGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1563 
                 Encoding 
                 TATAGTTATGGAGAAGGGTTCCTGACGGCTTTACCC 
               
               
                   
                 Probe 1516 
                 ATCAAAGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1564 
                 Encoding 
                 TATAGTTATGGAGAAGGGCCGGACGGCTTTACCCAT 
               
               
                   
                 Probe 1517 
                 CATAGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1565 
                 Encoding 
                 TATAGTTATGGAGAAGGGCAGGAAGGCCTGACGGCT 
               
               
                   
                 Probe 1518 
                 TTAAGGTGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1566 
                 Encoding 
                 TATAGTTATGGAGAAGGGTTCCTGACGGCTTTACCC 
               
               
                   
                 Probe 1519 
                 ATCTAAGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1567 
                 Encoding 
                 TATAGTTATGGAGAAGGGTCCTGACGGCTTTACCCA 
               
               
                   
                 Probe 1520 
                 TCAAAGGGAAGGGTATAGTTATGG 
               
               
                   
               
               
                 1568 
                 Encoding 
                 AATGATATGTTGAGTGGGAGCCCTGTCCACAGAGGT 
               
               
                   
                 Probe 1521 
                 TTAGTTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1569 
                 Encoding 
                 AATGATATGTTGAGTGGGAGGCACTGTTCGAGTGGA 
               
               
                   
                 Probe 1522 
                 ACATCAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1570 
                 Encoding 
                 AATGATATGTTGAGTGGGTCGATTTCTCCTTTGATAA 
               
               
                   
                 Probe 1523 
                 CAGAATCTACGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1571 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGTCTTCGTGTCTCCGA 
               
               
                   
                 Probe 1524 
                 AGACTCGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1572 
                 Encoding 
                 AATGATATGTTGAGTGGGAGGCACGGAAGGGTTCAT 
               
               
                   
                 Probe 1525 
                 CCCAGGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1573 
                 Encoding 
                 AATGATATGTTGAGTGGGAGGCACTGTTCGAGTGGA 
               
               
                   
                 Probe 1526 
                 ACAAGTAGGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1574 
                 Encoding 
                 AATGATATGTTGAGTGGGTAACCGTAGTATGCTGAC 
               
               
                   
                 Probe 1527 
                 CTAGCTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1575 
                 Encoding 
                 AATGATATGTTGAGTGGGTTTCAGTTTCAAAAGCAG 
               
               
                   
                 Probe 1528 
                 GTTTACGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1576 
                 Encoding 
                 GATAAGTAAGTAGGGATGAGCCCTGTCCACAGAGGT 
               
               
                   
                 Probe 1529 
                 TTAGTTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1577 
                 Encoding 
                 GATAAGTAAGTAGGGATGAGGCACTGTTCGAGTGGA 
               
               
                   
                 Probe 1530 
                 ACATCAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1578 
                 Encoding 
                 GATAAGTAAGTAGGGATGTCGATTTCTCCTTTGATA 
               
               
                   
                 Probe 1531 
                 ACAGAATCTACGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1579 
                 Encoding 
                 GATAAGTAAGTAGGGATGTGGTCTTCGTGTCTCCGA 
               
               
                   
                 Probe 1532 
                 AGACTCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1580 
                 Encoding 
                 GATAAGTAAGTAGGGATGAGGCACGGAAGGGTTCA 
               
               
                   
                 Probe 1533 
                 TCCCAGGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1581 
                 Encoding 
                 GATAAGTAAGTAGGGATGAGGCACTGTTCGAGTGGA 
               
               
                   
                 Probe 1534 
                 ACAAGTAGGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1582 
                 Encoding 
                 GATAAGTAAGTAGGGATGTAACCGTAGTATGCTGAC 
               
               
                   
                 Probe 1535 
                 CTAGCTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1583 
                 Encoding 
                 GATAAGTAAGTAGGGATGTTTCAGTTTCAAAAGCAG 
               
               
                   
                 Probe 1536 
                 GTTTACGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1584 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGACGTGGTCCGTAGACATT 
               
               
                   
                 Probe 1537 
                 ATGCCCAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1585 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTACTTCATCCGATAGTGC 
               
               
                   
                 Probe 1538 
                 AAGCAGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1586 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGATGCCCTAAGGCCTTCTT 
               
               
                   
                 Probe 1539 
                 CATAGTGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1587 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGAAACATCTGACTTAATTG 
               
               
                   
                 Probe 1540 
                 ACCGGGAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1588 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCCCAAGACCACAACC 
               
               
                   
                 Probe 1541 
                 TCTAAATCCTGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1589 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGAGCTATCTCTAAAGGA 
               
               
                   
                 Probe 1542 
                 TTCGCTCCTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1590 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGCGTTGTCTCAGCGTTCCC 
               
               
                   
                 Probe 1543 
                 GAACCGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1591 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCCTACGACAGACTTTA 
               
               
                   
                 Probe 1544 
                 TGAGTTGGCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1592 
                 Encoding 
                 GATAAGTAAGTAGGGATGACGTGGTCCGTAGACATT 
               
               
                   
                 Probe 1545 
                 ATGCCCAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1593 
                 Encoding 
                 GATAAGTAAGTAGGGATGTACTTCATCCGATAGTGC 
               
               
                   
                 Probe 1546 
                 AAGCAGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1594 
                 Encoding 
                 GATAAGTAAGTAGGGATGATGCCCTAAGGCCTTCTT 
               
               
                   
                 Probe 1547 
                 CATAGTGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1595 
                 Encoding 
                 GATAAGTAAGTAGGGATGAAACATCTGACTTAATTG 
               
               
                   
                 Probe 1548 
                 ACCGGGAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1596 
                 Encoding 
                 GATAAGTAAGTAGGGATGGCCCAAGACCACAACCTC 
               
               
                   
                 Probe 1549 
                 TAAATCCTGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1597 
                 Encoding 
                 GATAAGTAAGTAGGGATGGAGCTATCTCTAAAGGAT 
               
               
                   
                 Probe 1550 
                 TCGCTCCTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1598 
                 Encoding 
                 GATAAGTAAGTAGGGATGCGTTGTCTCAGCGTTCCC 
               
               
                   
                 Probe 1551 
                 GAACCGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1599 
                 Encoding 
                 GATAAGTAAGTAGGGATGGCCTACGACAGACTTTAT 
               
               
                   
                 Probe 1552 
                 GAGTTGGCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1600 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGGAAGGGAACAGGGCG 
               
               
                   
                 Probe 1553 
                 TTGCCGGAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1601 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGGAAAGGGAACAGGGC 
               
               
                   
                 Probe 1554 
                 GTTGCAGGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1602 
                 Encoding 
                 GGATAGAGTATAGTTGGGCGAGAAGGGAACAGGGC 
               
               
                   
                 Probe 1555 
                 GTTGAGGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1603 
                 Encoding 
                 GGATAGAGTATAGTTGGGTTCAACAGGGCGTTGCCC 
               
               
                   
                 Probe 1556 
                 CTGGCAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1604 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGCGCGAAGGGAACAGG 
               
               
                   
                 Probe 1557 
                 GCGTTCGGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1605 
                 Encoding 
                 GGATAGAGTATAGTTGGGCTTGAACAGGGCGTTGCC 
               
               
                   
                 Probe 1558 
                 CCTCGCGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1606 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGCACGAAGGGAACAGG 
               
               
                   
                 Probe 1559 
                 GCGTTGAGGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1607 
                 Encoding 
                 GGATAGAGTATAGTTGGGCGGGAAGGGAACAGGGC 
               
               
                   
                 Probe 1560 
                 GTTGCAGGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1608 
                 Encoding 
                 GATAAGTAAGTAGGGATGGGAAGGGAACAGGGCGT 
               
               
                   
                 Probe 1561 
                 TGCCGGAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1609 
                 Encoding 
                 GATAAGTAAGTAGGGATGGGAAAGGGAACAGGGCG 
               
               
                   
                 Probe 1562 
                 TTGCAGGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1610 
                 Encoding 
                 GATAAGTAAGTAGGGATGCGAGAAGGGAACAGGGC 
               
               
                   
                 Probe 1563 
                 GTTGAGGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1611 
                 Encoding 
                 GATAAGTAAGTAGGGATGTTCAACAGGGCGTTGCCC 
               
               
                   
                 Probe 1564 
                 CTGGCAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1612 
                 Encoding 
                 GATAAGTAAGTAGGGATGGCGCGAAGGGAACAGGG 
               
               
                   
                 Probe 1565 
                 CGTTCGGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1613 
                 Encoding 
                 GATAAGTAAGTAGGGATGCTTGAACAGGGCGTTGCC 
               
               
                   
                 Probe 1566 
                 CCTCGCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1614 
                 Encoding 
                 GATAAGTAAGTAGGGATGGCACGAAGGGAACAGGG 
               
               
                   
                 Probe 1567 
                 CGTTGAGGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1615 
                 Encoding 
                 GATAAGTAAGTAGGGATGCGGGAAGGGAACAGGGC 
               
               
                   
                 Probe 1568 
                 GTTGCAGGTGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1616 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGAAGACCGTAATCTTCCC 
               
               
                   
                 Probe 1569 
                 TTCACAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1617 
                 Encoding 
                 TGTAATAGTAAGGAGGGATCTTTCCGACCGTAATCT 
               
               
                   
                 Probe 1570 
                 TCCGAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1618 
                 Encoding 
                 TGTAATAGTAAGGAGGGATGATGCACAGATCTTCCG 
               
               
                   
                 Probe 1571 
                 ACCCATGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1619 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAGACGACCGTAATCTTCC 
               
               
                   
                 Probe 1572 
                 CTTCACAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1620 
                 Encoding 
                 TGTAATAGTAAGGAGGGAATTTTCCCTTCTGTACAC 
               
               
                   
                 Probe 1573 
                 CCGTAGCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1621 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGTGATGCACAGATCTTCC 
               
               
                   
                 Probe 1574 
                 GACCCATGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1622 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGTCCTTCCGACCGTAATC 
               
               
                   
                 Probe 1575 
                 TTCCGAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1623 
                 Encoding 
                 TGTAATAGTAAGGAGGGAATTTTCCCTTCTGTACAC 
               
               
                   
                 Probe 1576 
                 CCGAAGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1624 
                 Encoding 
                 GATAAGTAAGTAGGGATGGAAGACCGTAATCTTCCC 
               
               
                   
                 Probe 1577 
                 TTCACAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1625 
                 Encoding 
                 GATAAGTAAGTAGGGATGTCTTTCCGACCGTAATCT 
               
               
                   
                 Probe 1578 
                 TCCGAAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1626 
                 Encoding 
                 GATAAGTAAGTAGGGATGTGATGCACAGATCTTCCG 
               
               
                   
                 Probe 1579 
                 ACCCATGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1627 
                 Encoding 
                 GATAAGTAAGTAGGGATGAGACGACCGTAATCTTCC 
               
               
                   
                 Probe 1580 
                 CTTCACAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1628 
                 Encoding 
                 GATAAGTAAGTAGGGATGATTTTCCCTTCTGTACAC 
               
               
                   
                 Probe 1581 
                 CCGTAGCGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1629 
                 Encoding 
                 GATAAGTAAGTAGGGATGGTGATGCACAGATCTTCC 
               
               
                   
                 Probe 1582 
                 GACCCATGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1630 
                 Encoding 
                 GATAAGTAAGTAGGGATGGTCCTTCCGACCGTAATC 
               
               
                   
                 Probe 1583 
                 TTCCGAAGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1631 
                 Encoding 
                 GATAAGTAAGTAGGGATGATTTTCCCTTCTGTACAC 
               
               
                   
                 Probe 1584 
                 CCGAAGGGTGGAGGATAAGTAAGT 
               
               
                   
               
               
                 1632 
                 Encoding 
                 AATGATATGTTGAGTGGGCGTCTGTTTCCTGTTACCG 
               
               
                   
                 Probe 1585 
                 TTGCTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1633 
                 Encoding 
                 AATGATATGTTGAGTGGGCGGTCGTCAGCGAAACAG 
               
               
                   
                 Probe 1586 
                 CAACGAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1634 
                 Encoding 
                 AATGATATGTTGAGTGGGTGTCAAACAGCAAGCTGT 
               
               
                   
                 Probe 1587 
                 TTCCACAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1635 
                 Encoding 
                 AATGATATGTTGAGTGGGTTGCAAGCTGTTTCCTGTT 
               
               
                   
                 Probe 1588 
                 ACGCAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1636 
                 Encoding 
                 AATGATATGTTGAGTGGGAGTGAAACAGCAAGCTGT 
               
               
                   
                 Probe 1589 
                 TTCGACGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1637 
                 Encoding 
                 AATGATATGTTGAGTGGGTGTAAGCTGTTTCCTGTTA 
               
               
                   
                 Probe 1590 
                 CCCAAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1638 
                 Encoding 
                 AATGATATGTTGAGTGGGCGTCTGTTTCCTGTTACCG 
               
               
                   
                 Probe 1591 
                 TTCCTGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1639 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGTCGTCAGCGAAACAG 
               
               
                   
                 Probe 1592 
                 CAAGGACGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1640 
                 Encoding 
                 AGTATTATTAGGGTGAGGCGTCTGTTTCCTGTTACCG 
               
               
                   
                 Probe 1593 
                 TTGCTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1641 
                 Encoding 
                 AGTATTATTAGGGTGAGGCGGTCGTCAGCGAAACAG 
               
               
                   
                 Probe 1594 
                 CAACGAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1642 
                 Encoding 
                 AGTATTATTAGGGTGAGGGTCAAACAGCAAGCTGTT 
               
               
                   
                 Probe 1595 
                 TCCACAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1643 
                 Encoding 
                 AGTATTATTAGGGTGAGGTTGCAAGCTGTTTCCTGTT 
               
               
                   
                 Probe 1596 
                 ACGCAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1644 
                 Encoding 
                 AGTATTATTAGGGTGAGGAGTGAAACAGCAAGCTGT 
               
               
                   
                 Probe 1597 
                 TTCGACGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1645 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGTAAGCTGTTTCCTGTTA 
               
               
                   
                 Probe 1598 
                 CCCAAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1646 
                 Encoding 
                 AGTATTATTAGGGTGAGGCGTCTGTTTCCTGTTACCG 
               
               
                   
                 Probe 1599 
                 TTCCTGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1647 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGGTCGTCAGCGAAACAG 
               
               
                   
                 Probe 1600 
                 CAAGGACGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1648 
                 Encoding 
                 TGTATAGGATTAGAAGGGAGACATACTCTAGCTCGT 
               
               
                   
                 Probe 1601 
                 CAGAAAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1649 
                 Encoding 
                 TGTATAGGATTAGAAGGGTTTGCAAAGTATTAATTT 
               
               
                   
                 Probe 1602 
                 ACTGCCCTAGGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1650 
                 Encoding 
                 TGTATAGGATTAGAAGGGTTTAGCAAAGTATTAATT 
               
               
                   
                 Probe 1603 
                 TACTGCCCAAGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1651 
                 Encoding 
                 TGTATAGGATTAGAAGGGATGTAGCTCGTCAGTTTT 
               
               
                   
                 Probe 1604 
                 GAAACGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1652 
                 Encoding 
                 TGTATAGGATTAGAAGGGTTTAGCAAAGTATTAATT 
               
               
                   
                 Probe 1605 
                 TACTGCCGAAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1653 
                 Encoding 
                 TGTATAGGATTAGAAGGGATGTAGCTCGTCAGTTTT 
               
               
                   
                 Probe 1606 
                 GAATCGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1654 
                 Encoding 
                 TGTATAGGATTAGAAGGGTTTGCAAAGTATTAATTT 
               
               
                   
                 Probe 1607 
                 ACTGCCCAAGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1655 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGAAGCTCGTCAGTTTTG 
               
               
                   
                 Probe 1608 
                 AATCGTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1656 
                 Encoding 
                 AGTATTATTAGGGTGAGGAGACATACTCTAGCTCGT 
               
               
                   
                 Probe 1609 
                 CAGAAAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1657 
                 Encoding 
                 AGTATTATTAGGGTGAGGTTTGCAAAGTATTAATTT 
               
               
                   
                 Probe 1610 
                 ACTGCCCTAGGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1658 
                 Encoding 
                 AGTATTATTAGGGTGAGGTTTAGCAAAGTATTAATT 
               
               
                   
                 Probe 1611 
                 TACTGCCCAAGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1659 
                 Encoding 
                 AGTATTATTAGGGTGAGGATGTAGCTCGTCAGTTTT 
               
               
                   
                 Probe 1612 
                 GAAACGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1660 
                 Encoding 
                 AGTATTATTAGGGTGAGGTTTAGCAAAGTATTAATT 
               
               
                   
                 Probe 1613 
                 TACTGCCGAAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1661 
                 Encoding 
                 AGTATTATTAGGGTGAGGATGTAGCTCGTCAGTTTT 
               
               
                   
                 Probe 1614 
                 GAATCGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1662 
                 Encoding 
                 AGTATTATTAGGGTGAGGTTTGCAAAGTATTAATTT 
               
               
                   
                 Probe 1615 
                 ACTGCCCAAGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1663 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGAAGCTCGTCAGTTTTG 
               
               
                   
                 Probe 1616 
                 AATCGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1664 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGCAGCCAGTAAACTGGC 
               
               
                   
                 Probe 1617 
                 AGAAAGGTGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1665 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGTGTAGACAACTGCCTC 
               
               
                   
                 Probe 1618 
                 CCTTCGCGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1666 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGCCTGGCAACTGGACGT 
               
               
                   
                 Probe 1619 
                 AGGCCAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1667 
                 Encoding 
                 GGATAGAGTATAGTTGGGAGATCCCGTTCGCTACCC 
               
               
                   
                 Probe 1620 
                 ACGGAAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1668 
                 Encoding 
                 GGATAGAGTATAGTTGGGTCGGAGCATTGTTAAGAG 
               
               
                   
                 Probe 1621 
                 GCCAGAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1669 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGGCACTAACCTTTCCTA 
               
               
                   
                 Probe 1622 
                 ATTTCCACGGCGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1670 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGTGGACTTAAGCGCCCA 
               
               
                   
                 Probe 1623 
                 CCTAGCGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1671 
                 Encoding 
                 GGATAGAGTATAGTTGGGCCACGTCATACACAAAAC 
               
               
                   
                 Probe 1624 
                 TATTCGCAAACGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1672 
                 Encoding 
                 AGTATTATTAGGGTGAGGGCAGCCAGTAAACTGGCA 
               
               
                   
                 Probe 1625 
                 GAAAGGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1673 
                 Encoding 
                 AGTATTATTAGGGTGAGGGTGTAGACAACTGCCTCC 
               
               
                   
                 Probe 1626 
                 CTTCGCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1674 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGCCTGGCAACTGGACGT 
               
               
                   
                 Probe 1627 
                 AGGCCAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1675 
                 Encoding 
                 AGTATTATTAGGGTGAGGAGATCCCGTTCGCTACCC 
               
               
                   
                 Probe 1628 
                 ACGGAAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1676 
                 Encoding 
                 AGTATTATTAGGGTGAGGTCGGAGCATTGTTAAGAG 
               
               
                   
                 Probe 1629 
                 GCCAGAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1677 
                 Encoding 
                 AGTATTATTAGGGTGAGGTGGCACTAACCTTTCCTA 
               
               
                   
                 Probe 1630 
                 ATTTCCACGGCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1678 
                 Encoding 
                 AGTATTATTAGGGTGAGGGTGGACTTAAGCGCCCAC 
               
               
                   
                 Probe 1631 
                 CTAGCGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1679 
                 Encoding 
                 AGTATTATTAGGGTGAGGCCACGTCATACACAAAAC 
               
               
                   
                 Probe 1632 
                 TATTCGCAAACGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1680 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAGTGGATTGCTCCTTTGA 
               
               
                   
                 Probe 1633 
                 TTATCTTCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1681 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGAGCTACCGTCATCATCT 
               
               
                   
                 Probe 1634 
                 TCAGTCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1682 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGAGCCTCGTTAGCGGGAT 
               
               
                   
                 Probe 1635 
                 GTCTTCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1683 
                 Encoding 
                 TGTAATAGTAAGGAGGGACTAACAAGAATCAATAG 
               
               
                   
                 Probe 1636 
                 CAAGCATTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1684 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAGATACCGTCATCATCTT 
               
               
                   
                 Probe 1637 
                 CACTCTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1685 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGCTTGGGACCATTTTTAG 
               
               
                   
                 Probe 1638 
                 GGTAAAGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1686 
                 Encoding 
                 TGTAATAGTAAGGAGGGACTAACAAGAATCAATAG 
               
               
                   
                 Probe 1639 
                 CAAGCTTTTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1687 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGTCGATTGCTCCTTTGAT 
               
               
                   
                 Probe 1640 
                 TATCATCGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1688 
                 Encoding 
                 AGTATTATTAGGGTGAGGAGTGGATTGCTCCTTTGA 
               
               
                   
                 Probe 1641 
                 TTATCTTCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1689 
                 Encoding 
                 AGTATTATTAGGGTGAGGGAGCTACCGTCATCATCT 
               
               
                   
                 Probe 1642 
                 TCAGTCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1690 
                 Encoding 
                 AGTATTATTAGGGTGAGGGAGCCTCGTTAGCGGGAT 
               
               
                   
                 Probe 1643 
                 GTCTTCGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1691 
                 Encoding 
                 AGTATTATTAGGGTGAGGCTAACAAGAATCAATAGC 
               
               
                   
                 Probe 1644 
                 AAGCATTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1692 
                 Encoding 
                 AGTATTATTAGGGTGAGGAGATACCGTCATCATCTT 
               
               
                   
                 Probe 1645 
                 CACTCTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1693 
                 Encoding 
                 AGTATTATTAGGGTGAGGGCTTGGGACCATTTTTAG 
               
               
                   
                 Probe 1646 
                 GGTAAAGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1694 
                 Encoding 
                 AGTATTATTAGGGTGAGGCTAACAAGAATCAATAGC 
               
               
                   
                 Probe 1647 
                 AAGCTTTTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1695 
                 Encoding 
                 AGTATTATTAGGGTGAGGGTCGATTGCTCCTTTGATT 
               
               
                   
                 Probe 1648 
                 ATCATCGTGGGTTGGAGTATTATTAG 
               
               
                   
               
               
                 1696 
                 Encoding 
                 AATGATATGTTGAGTGGGCCGCTCCTATAGCATGAG 
               
               
                   
                 Probe 1649 
                 GCCTACGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1697 
                 Encoding 
                 AATGATATGTTGAGTGGGTGGATCGTAGCAACTAGA 
               
               
                   
                 Probe 1650 
                 GACAAGCCAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1698 
                 Encoding 
                 AATGATATGTTGAGTGGGTCGCTGTGTCCACTTTCTC 
               
               
                   
                 Probe 1651 
                 TTTCCTCGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1699 
                 Encoding 
                 AATGATATGTTGAGTGGGAAACATCGGTCTTGCACA 
               
               
                   
                 Probe 1652 
                 ACCCGGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1700 
                 Encoding 
                 AATGATATGTTGAGTGGGTAGGCAAGCTAGATCATG 
               
               
                   
                 Probe 1653 
                 CTGCGCAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1701 
                 Encoding 
                 AATGATATGTTGAGTGGGTAGGCAAGCTAGATCATG 
               
               
                   
                 Probe 1654 
                 CTGGGCGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1702 
                 Encoding 
                 AATGATATGTTGAGTGGGTAGCACCTAATATTAGTA 
               
               
                   
                 Probe 1655 
                 AGTGCGTAAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1703 
                 Encoding 
                 AATGATATGTTGAGTGGGCTGCGATGCATTTTCTGG 
               
               
                   
                 Probe 1656 
                 GATATCGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1704 
                 Encoding 
                 ATGTATTAAGAGGAGGGACCGCTCCTATAGCATGAG 
               
               
                   
                 Probe 1657 
                 GCCTACGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1705 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGGATCGTAGCAACTAGA 
               
               
                   
                 Probe 1658 
                 GACAAGCCAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1706 
                 Encoding 
                 ATGTATTAAGAGGAGGGATCGCTGTGTCCACTTTCT 
               
               
                   
                 Probe 1659 
                 CTTTCCTCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1707 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAAACATCGGTCTTGCACA 
               
               
                   
                 Probe 1660 
                 ACCCGGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1708 
                 Encoding 
                 ATGTATTAAGAGGAGGGATAGGCAAGCTAGATCATG 
               
               
                   
                 Probe 1661 
                 CTGCGCAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1709 
                 Encoding 
                 ATGTATTAAGAGGAGGGATAGGCAAGCTAGATCATG 
               
               
                   
                 Probe 1662 
                 CTGGGCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1710 
                 Encoding 
                 ATGTATTAAGAGGAGGGATAGCACCTAATATTAGTA 
               
               
                   
                 Probe 1663 
                 AGTGCGTAAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1711 
                 Encoding 
                 ATGTATTAAGAGGAGGGACTGCGATGCATTTTCTGG 
               
               
                   
                 Probe 1664 
                 GATATCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1712 
                 Encoding 
                 TGTATAGGATTAGAAGGGTTTGCCTTTCAACTTTCTT 
               
               
                   
                 Probe 1665 
                 CCATGGCCGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1713 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGGTCGGAAAATAGTGTT 
               
               
                   
                 Probe 1666 
                 ATACGGATAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1714 
                 Encoding 
                 TGTATAGGATTAGAAGGGAGGGCGGAAAATAGTGTT 
               
               
                   
                 Probe 1667 
                 ATACGCATGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1715 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGCTGGGAAGCTCTATCT 
               
               
                   
                 Probe 1668 
                 CTAGACACGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1716 
                 Encoding 
                 TGTATAGGATTAGAAGGGTGTATACTCTCATCCTTGT 
               
               
                   
                 Probe 1669 
                 TCTTCAGAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1717 
                 Encoding 
                 TGTATAGGATTAGAAGGGAGGGCGGAAAATAGTGTT 
               
               
                   
                 Probe 1670 
                 ATACGGATAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1718 
                 Encoding 
                 TGTATAGGATTAGAAGGGAAAGAGATTAGCTTAGCC 
               
               
                   
                 Probe 1671 
                 TCGGCTGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1719 
                 Encoding 
                 TGTATAGGATTAGAAGGGTAAACTCTCATCCTTGTTC 
               
               
                   
                 Probe 1672 
                 TTCAGAGGGTGAGTGTATAGGATT 
               
               
                   
               
               
                 1720 
                 Encoding 
                 ATGTATTAAGAGGAGGGATTTGCCTTTCAACTTTCTT 
               
               
                   
                 Probe 1673 
                 CCATGGCCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1721 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGGTCGGAAAATAGTGTTA 
               
               
                   
                 Probe 1674 
                 TACGGATAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1722 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAGGGCGGAAAATAGTGT 
               
               
                   
                 Probe 1675 
                 TATACGCATGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1723 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGCTGGGAAGCTCTATCTC 
               
               
                   
                 Probe 1676 
                 TAGACACGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1724 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGTATACTCTCATCCTTGT 
               
               
                   
                 Probe 1677 
                 TCTTCAGAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1725 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAGGGCGGAAAATAGTGT 
               
               
                   
                 Probe 1678 
                 TATACGGATAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1726 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAAAGAGATTAGCTTAGCC 
               
               
                   
                 Probe 1679 
                 TCGGCTGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1727 
                 Encoding 
                 ATGTATTAAGAGGAGGGATAAACTCTCATCCTTGTT 
               
               
                   
                 Probe 1680 
                 CTTCAGAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1728 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGTAGCTCCCGGGTGCTT 
               
               
                   
                 Probe 1681 
                 ATGCCCAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1729 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCGCTAAAGCAAACAC 
               
               
                   
                 Probe 1682 
                 ACTTCCTAGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1730 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCGCTAAAGCAAACAC 
               
               
                   
                 Probe 1683 
                 ACTTCCAAGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1731 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGACTCGTTATTTCTCGGAT 
               
               
                   
                 Probe 1684 
                 TCGGAGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1732 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGCGGTAAAGCAAACACAC 
               
               
                   
                 Probe 1685 
                 TTCCTAGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1733 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTCTTTATTTCTCGGATTC 
               
               
                   
                 Probe 1686 
                 GCTGGCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1734 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCGGTGTTCCTCCTGAT 
               
               
                   
                 Probe 1687 
                 CTCTTGCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1735 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGCGTCGCTCCCGGGTGCTT 
               
               
                   
                 Probe 1688 
                 ATGGCCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1736 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGTAGCTCCCGGGTGCTTA 
               
               
                   
                 Probe 1689 
                 TGCCCAGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1737 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGCGCTAAAGCAAACACA 
               
               
                   
                 Probe 1690 
                 CTTCCTAGGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1738 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGCGCTAAAGCAAACACA 
               
               
                   
                 Probe 1691 
                 CTTCCAAGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1739 
                 Encoding 
                 ATGTATTAAGAGGAGGGAACTCGTTATTTCTCGGAT 
               
               
                   
                 Probe 1692 
                 TCGGAGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1740 
                 Encoding 
                 ATGTATTAAGAGGAGGGACGGTAAAGCAAACACAC 
               
               
                   
                 Probe 1693 
                 TTCCTAGGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1741 
                 Encoding 
                 ATGTATTAAGAGGAGGGATCTTTATTTCTCGGATTCG 
               
               
                   
                 Probe 1694 
                 CTGGCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1742 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGCGGTGTTCCTCCTGATC 
               
               
                   
                 Probe 1695 
                 TCTTGCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1743 
                 Encoding 
                 ATGTATTAAGAGGAGGGACGTCGCTCCCGGGTGCTT 
               
               
                   
                 Probe 1696 
                 ATGGCCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1744 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGGCGCTCTCATTCTTAAT 
               
               
                   
                 Probe 1697 
                 ATCTTCGTAGCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1745 
                 Encoding 
                 TGTAATAGTAAGGAGGGATTCTATCTCTACGCCTGT 
               
               
                   
                 Probe 1698 
                 CATGTCGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1746 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGGTCTGCACCGAATAAAT 
               
               
                   
                 Probe 1699 
                 CCTAAGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1747 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGTACTTTCGTCCCTGTTG 
               
               
                   
                 Probe 1700 
                 ATACTTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1748 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAGGATGCAATCCTCGGGT 
               
               
                   
                 Probe 1701 
                 TAACGGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1749 
                 Encoding 
                 TGTAATAGTAAGGAGGGACAATGTGATTTGCTTAAC 
               
               
                   
                 Probe 1702 
                 GTCCGGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1750 
                 Encoding 
                 TGTAATAGTAAGGAGGGAAGTGACTTCGGGTGCTTC 
               
               
                   
                 Probe 1703 
                 CAAGAGTGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1751 
                 Encoding 
                 TGTAATAGTAAGGAGGGAGTAGGGATTCCTCCCCGA 
               
               
                   
                 Probe 1704 
                 CACAATGGGTGAGTGTAATAGTAA 
               
               
                   
               
               
                 1752 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGGCGCTCTCATTCTTAAT 
               
               
                   
                 Probe 1705 
                 ATCTTCGTAGCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1753 
                 Encoding 
                 ATGTATTAAGAGGAGGGATTCTATCTCTACGCCTGT 
               
               
                   
                 Probe 1706 
                 CATGTCGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1754 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGGTCTGCACCGAATAAAT 
               
               
                   
                 Probe 1707 
                 CCTAAGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1755 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGTACTTTCGTCCCTGTTG 
               
               
                   
                 Probe 1708 
                 ATACTTGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1756 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAGGATGCAATCCTCGGGT 
               
               
                   
                 Probe 1709 
                 TAACGGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1757 
                 Encoding 
                 ATGTATTAAGAGGAGGGACAATGTGATTTGCTTAAC 
               
               
                   
                 Probe 1710 
                 GTCCGGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1758 
                 Encoding 
                 ATGTATTAAGAGGAGGGAAGTGACTTCGGGTGCTTC 
               
               
                   
                 Probe 1711 
                 CAAGAGGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1759 
                 Encoding 
                 ATGTATTAAGAGGAGGGAGTAGGGATTCCTCCCCGA 
               
               
                   
                 Probe 1712 
                 CACAATGAGGAGGATGTATTAAGA 
               
               
                   
               
               
                 1760 
                 Encoding 
                 AATGATATGTTGAGTGGGTTTTTCTCTCCAATTTGTA 
               
               
                   
                 Probe 1713 
                 ACGAAGAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1761 
                 Encoding 
                 AATGATATGTTGAGTGGGTGATGCCTCTATATAGTT 
               
               
                   
                 Probe 1714 
                 GGCTGTGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1762 
                 Encoding 
                 AATGATATGTTGAGTGGGTTATCTCTCCAATTTGTAA 
               
               
                   
                 Probe 1715 
                 CGAAGAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1763 
                 Encoding 
                 AATGATATGTTGAGTGGGAGGCTAGTATCATGTGAT 
               
               
                   
                 Probe 1716 
                 ACTTATGGGTAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1764 
                 Encoding 
                 AATGATATGTTGAGTGGGTGAACCACTAGTATCATG 
               
               
                   
                 Probe 1717 
                 TGATACTATAGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1765 
                 Encoding 
                 AATGATATGTTGAGTGGGAAACTCCATATCACTACT 
               
               
                   
                 Probe 1718 
                 TAGCTTAGGGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1766 
                 Encoding 
                 AATGATATGTTGAGTGGGTGCTGCACAGATTACTTA 
               
               
                   
                 Probe 1719 
                 ATATAACCTTGTGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1767 
                 Encoding 
                 AATGATATGTTGAGTGGGTGTAACCACCTGTATAGA 
               
               
                   
                 Probe 1720 
                 CGTCGGCGTGGTGGAATGATATGTT 
               
               
                   
               
               
                 1768 
                 Encoding 
                 TAGAATTAGAGAGATGGGTTTTTCTCTCCAATTTGTA 
               
               
                   
                 Probe 1721 
                 ACGAAGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1769 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGATGCCTCTATATAGTT 
               
               
                   
                 Probe 1722 
                 GGCTGTGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1770 
                 Encoding 
                 TAGAATTAGAGAGATGGGTTATCTCTCCAATTTGTA 
               
               
                   
                 Probe 1723 
                 ACGAAGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1771 
                 Encoding 
                 TAGAATTAGAGAGATGGGAGGCTAGTATCATGTGAT 
               
               
                   
                 Probe 1724 
                 ACTTATGGGTAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1772 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGAACCACTAGTATCATG 
               
               
                   
                 Probe 1725 
                 TGATACTATAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1773 
                 Encoding 
                 TAGAATTAGAGAGATGGGAAACTCCATATCACTACT 
               
               
                   
                 Probe 1726 
                 TAGCTTAGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1774 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGCTGCACAGATTACTTA 
               
               
                   
                 Probe 1727 
                 ATATAACCTTGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1775 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGTAACCACCTGTATAGA 
               
               
                   
                 Probe 1728 
                 CGTCGGCGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1776 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCACCGGGAGCCTTTGG 
               
               
                   
                 Probe 1729 
                 CACGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1777 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGGCTCGGCTTTTCACCC 
               
               
                   
                 Probe 1730 
                 CGAAGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1778 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGAAACTTCCGACTTGTATT 
               
               
                   
                 Probe 1731 
                 GCCCAGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1779 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGCGCTCAGTCAATTAAC 
               
               
                   
                 Probe 1732 
                 ATTCCAAGGTGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1780 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGTGGCAACTTCCTCTTAAT 
               
               
                   
                 Probe 1733 
                 TGCTTCCGAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1781 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGAGCAGCTCCCTGCTTTCG 
               
               
                   
                 Probe 1734 
                 CTTCCCGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1782 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGACGAGCTTTCTCTGTTTG 
               
               
                   
                 Probe 1735 
                 CTAGACGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1783 
                 Encoding 
                 ATGGAAGTAGTAGAAGGGACGCGTAGGGAACAGAA 
               
               
                   
                 Probe 1736 
                 TGTTTGAGGGATGTATGGAAGTAGT 
               
               
                   
               
               
                 1784 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGCACCGGGAGCCTTTGG 
               
               
                   
                 Probe 1737 
                 CACGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1785 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGCTCGGCTTTTCACCC 
               
               
                   
                 Probe 1738 
                 CGAAGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1786 
                 Encoding 
                 TAGAATTAGAGAGATGGGAAACTTCCGACTTGTATT 
               
               
                   
                 Probe 1739 
                 GCCCAGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1787 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGCGCTCAGTCAATTAAC 
               
               
                   
                 Probe 1740 
                 ATTCCAAGGTGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1788 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGGCAACTTCCTCTTAAT 
               
               
                   
                 Probe 1741 
                 TGCTTCCGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1789 
                 Encoding 
                 TAGAATTAGAGAGATGGGAGCAGCTCCCTGCTTTCG 
               
               
                   
                 Probe 1742 
                 CTTCCCGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1790 
                 Encoding 
                 TAGAATTAGAGAGATGGGACGAGCTTTCTCTGTTTG 
               
               
                   
                 Probe 1743 
                 CTAGACGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1791 
                 Encoding 
                 TAGAATTAGAGAGATGGGACGCGTAGGGAACAGAA 
               
               
                   
                 Probe 1744 
                 TGTTTGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1792 
                 Encoding 
                 GGATAGAGTATAGTTGGGCGTCCCCTCTGTAAGCGG 
               
               
                   
                 Probe 1745 
                 ATTAGAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1793 
                 Encoding 
                 GGATAGAGTATAGTTGGGCCACCGTCAAATTTCTCT 
               
               
                   
                 Probe 1746 
                 TTCTAGAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1794 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGTGCCTATCGGCAACAC 
               
               
                   
                 Probe 1747 
                 TTAGATGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1795 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGCGTGTCTCCATAACTT 
               
               
                   
                 Probe 1748 
                 CGCACCCGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1796 
                 Encoding 
                 GGATAGAGTATAGTTGGGAAGCCATTTTATCAATGG 
               
               
                   
                 Probe 1749 
                 CAGTGATGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1797 
                 Encoding 
                 GGATAGAGTATAGTTGGGTCTGCTCCCTCATTTCTGT 
               
               
                   
                 Probe 1750 
                 TACCGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1798 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGTCTAAAATGCTTTTTCC 
               
               
                   
                 Probe 1751 
                 ATTGTGGAGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1799 
                 Encoding 
                 GGATAGAGTATAGTTGGGTGAAGTTCATCAGTATCT 
               
               
                   
                 Probe 1752 
                 TTTGCCCATGGGATGGAGGATAGAGTAT 
               
               
                   
               
               
                 1800 
                 Encoding 
                 TAGAATTAGAGAGATGGGCGTCCCCTCTGTAAGCGG 
               
               
                   
                 Probe 1753 
                 ATTAGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1801 
                 Encoding 
                 TAGAATTAGAGAGATGGGCCACCGTCAAATTTCTCT 
               
               
                   
                 Probe 1754 
                 TTCTAGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1802 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGTGCCTATCGGCAACAC 
               
               
                   
                 Probe 1755 
                 TTAGATGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1803 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGCGTGTCTCCATAACTT 
               
               
                   
                 Probe 1756 
                 CGCACCCGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1804 
                 Encoding 
                 TAGAATTAGAGAGATGGGAAGCCATTTTATCAATGG 
               
               
                   
                 Probe 1757 
                 CAGTGATGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1805 
                 Encoding 
                 TAGAATTAGAGAGATGGGTCTGCTCCCTCATTTCTGT 
               
               
                   
                 Probe 1758 
                 TACCGGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1806 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGTCTAAAATGCTTTTTC 
               
               
                   
                 Probe 1759 
                 CATTGTGGAGGTGGAGTAGAATTAGAG 
               
               
                   
               
               
                 1807 
                 Encoding 
                 TAGAATTAGAGAGATGGGTGAAGTTCATCAGTATCT 
               
               
                   
                 Probe 1760 
                 TTTGCCCATGGGTGGAGTAGAATTAGAG 
               
               
                   
               
            
           
         
       
     
     Although the present disclosure has been described with reference to the specific embodiments thereof, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the true spirit and scope of the disclosure. In addition, many modifications may be made to adapt a particular situation, material, composition of matter, process, process step or steps, to the objective, spirit and scope of the present disclosure. All such modifications are intended to be within the scope of the claims appended hereto.