Patent Publication Number: US-11644438-B2

Title: Capillary cartridge and electrophoresis apparatus

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application is a continuation of U.S. patent application Ser. No. 15/739,184, filed Dec. 22, 2017, which is a 371 of International Application No PCT/JP2015/069040, filed Jul. 1, 2015, the disclosures of all of which are expressly incorporated by reference herein. 
    
    
     TECHNICAL FIELD 
     The present invention relates to a capillary cartridge and an electrophoresis apparatus that uses the capillary cartridge, and particularly to a technology of improving attachability and heat dissipation. 
     BACKGROUND ART 
     Recently, DNA analysis has been rapidly expanding from a research application to a clinical field such as a hospital. There is a method of separating a DNA fragment by electrophoresis as means of the DNA analysis, and the DNA analysis is used for a criminal investigation, determination of a blood relationship, or a disease diagnosis. 
     In capillary electrophoresis, a capillary filled with a separation medium is maintained at a constant temperature and a high voltage is applied, and thereby, charged DNA is separated for each base length. A capillary is irradiated with excitation light and fluorescence emitted from the fluorescent dye label of DNA passing through the capillary is detected, and thereby, base sequence of a sample can be read. For example, Patent Documents 1 and 2 are related art documents relating to capillary electrophoresis. 
     Patent Document 1 discloses a capillary unit including a capillary, a frame for supporting the capillary, and a load header for holding a capillary cathode portion, and an electrophoresis apparatus that uses the capillary unit. The frame includes a separator for separating and holding the capillary, and can hold the capillary in a fixed shape as the capillary passes through the separator. 
     Patent Document 2 discloses an electrophoresis apparatus configured by a capillary, a support body in which a capillary is disposed on a surface, a heater for temperature control indirect contact with the capillary, an optical system, and a high-voltage power supply. By a structure in which the capillary is directly in contact with the heater, time required to increase temperature to a predetermined temperature can be shortened at the time of electrophoresis analysis. 
     CITATION LIST 
     Patent Literature 
     
         
         Patent Document 1: JP-A-2009-174897 
         Patent Document 2: JP-A-2006-284530 
       
    
     SUMMARY OF INVENTION 
     Technical Problem 
     Patent Document 1 provides a structure in which, when the capillary unit is attached, total three places of both end portions and a middle portion of the capillary of a detection unit are separately attached, and furthermore, it is necessary to close a cover or the like at each place, at least six steps are required, and an operation of a user is complicated. In addition, since only a part of the capillary is held by the frame, there is a risk of being damaged by applying excessive force at the time of attachment. 
     Patent Document 2 provides a structure in which the capillary is directly interposed between the heater and the support body. Accordingly, a structure is not provided in which, at the time of electrophoresis analysis, heat generated from the capillary is positively diffused when a high voltage is applied to both ends of the capillary. If the capillary is shortened to reduce an analysis time, the total resistance value of the capillary decreases and a flowing current increases, and thereby, the amount of heat generated from the capillary increases. Thus, if the structure does not positively diffuse the heat, the heat of the capillary cannot be dissipated, and an internal temperature of the capillary becomes higher than the predetermined temperature. As a result, electrophoresis speed of a sample becomes unstable, and separation performance decreases. Therefore, the time required for electrophoresis analysis cannot be reduced by the structure. 
     An object of the present invention is to provide a capillary cartridge and an electrophoresis apparatus which solve the above-described problems and improve heat dissipation performance for realizing improvement of attachability of a capillary and short time analysis. 
     Solution to Problem 
     In order to achieve the above-described object, the present invention provides a capillary cartridge which uses an electrophoresis apparatus including a capillary, a support body that supports the capillary, a capillary head that bundles one end of the capillary, an electrode that is provided in the other end of the capillary, a detection unit that is provided in a part of the capillary, and a heat dissipation body that is provided between the capillary and the support body. 
     In addition, In order to achieve the above-described object, the present invention provides an electrophoresis apparatus including a capillary cartridge having a capillary, a support body that supports the capillary, a capillary head that bundles one end portion of the capillary, an electrode that is provided in the other end portion of the capillary, a detection unit that is provided in a part of the capillary, and a heat dissipation body that is provided between the capillary and the support body, a thermostatic bath that holds the capillary at a predetermined temperature, an injection mechanism that injects an electrophoresis medium into the capillary, and an irradiation detection portion that performs irradiation and detection of light at the time of electrophoresis by using the capillary. 
     Advantageous Effects of Invention 
     According to the present invention, temperature increase inside the capillary can be suppressed by a heat dissipation body, and thus, it is possible to perform electrophoresis under a high voltage application condition in which the amount of heat increases and to reduce analysis time. In addition, it is possible to improve complexity of an operation by reducing the number of fixing places at the time of attachment by adopting a structure in which a capillary and a support body are integrated. Thereby, it is possible to improve analysis performance and to improve usability. 
    
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         FIG.  1    is a schematic view illustrating a configuration of a capillary electrophoresis apparatus according to embodiment 1. 
         FIG.  2    is a top view of the capillary electrophoresis apparatus according to embodiment 1. 
         FIG.  3    is a cross-sectional view taken along a line A-A of the capillary electrophoresis apparatus according to embodiment 1. 
         FIG.  4    is a view illustrating a configuration of a capillary cartridge according to embodiment 1. 
         FIG.  5    is an exploded view of the capillary cartridge according to embodiment 1. 
         FIG.  6    is a sectional view illustrating an example of a support body and a heat dissipation body according to embodiment 1. 
         FIG.  7    is a view illustrating attachment of the capillary cartridge according to embodiment 1. 
         FIG.  8    is a sectional view illustrating an operation of a clip according to embodiment 1. 
         FIG.  9    is a view illustrating an adjustment margin of the capillary according to embodiment 1. 
         FIG.  10    is a work flowchart of attachment of the capillary cartridge according to embodiment 1. 
         FIG.  11    is a sectional view illustrating a peripheral portion of the capillary according to embodiment 1. 
         FIG.  12    is a sectional view illustrating an example of a thermostatic bath door according to embodiment 1. 
         FIG.  13    is a view illustrating a configuration of a capillary cartridge according to embodiment 2. 
         FIG.  14    is a view illustrating a configuration of a capillary cartridge according to embodiment 3. 
         FIG.  15    is a view illustrating a configuration of a support body according to embodiment 4. 
         FIG.  16    is a sectional view illustrating a configuration of a support body according to embodiment 5. 
     
    
    
     DESCRIPTION OF EMBODIMENTS 
     Hereinafter, various embodiments of the present invention will be described with reference to the drawings. In all the drawings illustrating various embodiments, those having the same function are denoted by the same reference numerals or symbols. 
     Embodiment 1 
     Embodiment 1 is an embodiment of a capillary cartridge for improving heat dissipation performance for improving attachability and shortening time analysis, and an electrophoresis apparatus which uses the capillary cartridge. That is, Embodiment 1 is an embodiment of an electrophoresis apparatus including a capillary cartridge having a capillary, a plate-like support body that supports the capillary, a capillary head that bundles one end portion of the capillary, an electrode that is provided in the other end portion of the capillary, a detection unit that is provided in a part of the capillary, and a heat dissipation body that is provided between the capillary and the support body; a thermostatic bath that holds the capillary at a predetermined temperature; an injection mechanism that injects an electrophoresis medium into the capillary; and an irradiation detection portiont that performs irradiation and detection of light at the time of electrophoresis by using the capillary. 
     Hereinafter, configurations and dispositions of the capillary cartridge according to embodiment 1 and an electrophoresis apparatus which uses the capillary cartridge, and an attachment method will be described with reference to  FIG.  1    to  FIG.  9   .  FIG.  1    illustrates an apparatus configuration view of a capillary electrophoresis apparatus according to embodiment 1. The present apparatus can be roughly divided into two units: an irradiation detection/thermostatic bath unit  40  at the top of the apparatus and an autosampler unit  20  at the bottom of the apparatus. 
     In the autosampler unit  20  which is an injection mechanism described above, a Y-axis drive body  23  is mounted on a sampler base  21 , and can be driven on the Y axis. A Z-axis drive body  24  is mounted on the Y-axis drive body  23 , and can be driven on the Z axis. A sample tray  25  is mounted on the Z-axis drive body  24 , and a user sets an electrophoresis medium container  28 , an anode side buffer liquid container  29 , a cathode side buffer liquid container  33 , and a sample container  26  on the sample tray  25 . The sample container  26  is set on the X-axis drive body  22  mounted on the sample tray  25 , and only the sample container  26  can be driven to the X axis on the sample tray  25 . A liquid feeding mechanism  27  is also mounted on the Z-axis drive body  24 . The liquid feeding mechanism  27  is disposed below the electrophoresis medium container  28 . 
     The irradiation detection/thermostatic bath unit  40  includes a thermostatic bath unit  41  which is the above-mentioned thermostatic bath and a thermostatic bath door  43 , and can maintain an inside temperature at a constant temperature. An irradiation detection unit  42  which is the irradiation detection portion is mounted behind the thermostatic bath unit  41 , and can perform detection at the time of electrophoresis. A user sets a capillary cartridge  01  which will be described in detail below in the thermostatic bath unit  41 , the electrophoresis is performed while the capillary is maintained at a constant temperature in the thermostatic bath unit  41 , and detection is performed by the irradiation detection unit  42 . In addition, an electrode (anode)  44  is also mounted on the thermostatic bath unit  41  so as to drop a high voltage to GND when the high voltage for electrophoresis is applied. 
     As described above, the capillary cartridge  01  is fixed to the thermostatic bath unit  41 . The electrophoresis medium container  28 , the anode side buffer liquid container  29 , the cathode side buffer liquid container  33 , and the sample container  26  can be driven to the YZ axis by the autosampler unit  20 , and only the sample container  26  is further driven to the X axis. The electrophoresis medium container  28 , the anode side buffer liquid container  29 , the cathode side buffer liquid container  33 , and the sample container  26  can be automatically connected to an arbitrary position by movement of the autosampler unit  20 , in the capillary of the fixed capillary cartridge  01 . 
       FIG.  2    illustrates a view, which is viewed from the top, of the capillary electrophoresis apparatus illustrated in  FIG.  1   . The anode side buffer liquid container  29  set on the sample tray  25  includes an anode side wash layer  30 , an anode side electrophoresis buffer liquid layer  31 , and an anode side sample introduction buffer liquid layer  32 . In addition, the cathode side buffer liquid container  33  includes a waste liquid layer  34 , a cathode side wash layer  35 , and a cathode side electrophoresis buffer liquid layer  36 . 
     The electrophoresis medium container  28 , the anode side buffer liquid container  29 , the cathode side buffer liquid container  33 , and the sample container  26  are arranged in a positional relationship as illustrated. Thereby, a positional relationship of anode side-cathode side at the time of connection with a capillary  02  of the capillary cartridge in the thermostatic bath unit  41  becomes “electrophoresis medium container  28 -waste liquid layer  34 ”, “anode side wash layer  30 -cathode side wash layer  35 ”, “anode side electrophoresis buffer liquid layer  31 -cathode side electrophoresis buffer liquid layer  36 ”, and “anode side sample introduction buffer liquid layer  32 -sample container  26 ”. 
       FIG.  3    is a cross-sectional view taken along a line A-A of  FIG.  2   . The electrophoresis medium container  28  is set in the sample tray  25 . In addition, the liquid feeding mechanism  27  is disposed such that a plunger embedded in the liquid feeding mechanism  27  is located below the electrophoresis medium container  28 . 
     At the time of electrophoresis, the right side of the capillary  02  in  FIG.  3    becomes a cathode side, and the left side becomes an anode side. The autosampler unit  20  moves to a location of “anode side electrophoresis buffer liquid layer  31 -cathode side electrophoresis buffer liquid layer  36 ”, a high voltage is applied to the capillary  02  on the cathode side, and a current flows from the electrode (anode)  44  to GND through the cathode side buffer liquid container  33  and the anode side buffer liquid container  29 , and thereby, electrophoresis is performed. An apparatus structure may be provided in which a location of the sample tray  25  is fixed and the irradiation detection/thermostatic bath unit  40  is operated. 
       FIG.  4    illustrates a schematic view of a configuration of the capillary cartridge according to the present embodiment. The capillary cartridge  01  is configured with the capillary  02 , a support body  03 , a heat dissipation body  04 , an electrode holder  05 , a detection unit  06 , a capillary head  07 , an electrode (cathode)  08 , and a handle  09  as a grip portion. In addition, the electrode (cathode)  08  may be directly fixed to the support body  03 . In  FIG.  4   , the capillary cartridge  01  is arranged in the order of the support body  03  having the handle  09 , the heat dissipation body  04 , and the capillary  02  from the front side of  FIG.  4   . 
     The capillary head  07  is an end portion of the capillary  02 , and is an injection end or a discharge end that holds the capillary  02  in a bundle and fills the electrophoresis medium. In the present embodiment, when the capillary cartridge  01  is attached to the electrophoresis apparatus, the capillary head  07  is connected to a container storing the electrophoresis medium, and thereby, the capillary cartridge functions as an injection end. The capillary head is installed in a state of being bent in the electrophoresis apparatus. 
       FIG.  5    illustrates an exploded view of the capillary cartridge in the present embodiment illustrated in  FIG.  4   . The heat dissipation body  04  is attached to the support body  03  by the adhesiveness or tackiness of the heat dissipation body  04 , chemical adhesion, a physical attachment mechanism, or the like. In addition, the capillary  02  has an integral structure by attaching the electrode holder  05  and the detection unit  06  to the support body  03 . The electrode holder  05  holds an electrode (cathode)  08 , and has a structure in which an electrode holder fixing pin  10  formed in the electrode holder  05  passes through the electrode holder fixing hole  11  of the support body  03  thereby being fixed to the support body  03 . In addition, the support body  03  includes a detection unit fixing frame  12  for fixing the detection unit  06 , and the detection unit  06  is fixed to the support body  03  by being fit into the detection unit fixing frame  12  formed in the support body  03 . Numeric signs  14  and  16  are a positioning hole into which a detection unit positioning pin is inserted, and an electrode holder positioning hole, respectively. 
     The capillary  02  is a stagnant flow channel coated with a coating for maintaining light shielding and a strength, and is, for example, a quartz glass tube that has an inner diameter of approximately 50 μm and is coated with polyimide. The tube is filled with electrophoresis medium, thereby, becoming an electrophoresis path separating a sample. Since the capillary  02  and the heat dissipation body  04  are in close contact with each other, heat generated from the capillary  02  at the time of applying a high voltage can be released to the support body  03  side by the heat dissipation body  04 , and a temperature inside the capillary  02  can be prevented from increasing. 
     The electrode (cathode)  08  exists in correspondence with the number of the capillaries  02 , a charged sample can be introduced into the capillary  02  by applying a voltage, and thereby, electrophoresis separation can be performed for each molecular size. The electrode (cathode)  08  is a stainless steel pipe having an inner diameter of, for example, approximately 0.1 to 0.5 m, and the capillary  02  is inserted therein. 
     The detection unit  06  is located at an intermediate portion of the capillary  02 , and the capillaries  02  are arranged in a planar manner with constant accuracy. The detection unit  06  is a portion for detecting fluorescence of a sample passing through the capillary  02 , and it is necessary to perform positioning with high accuracy with respect to a location of a detection system of a device. 
       FIG.  6    illustrates a sectional view of the support body  03  and the heat dissipation body  04  of the capillary cartridge according to the present embodiment. The heat dissipation body  04  is, for example, a soft silicon rubber with heat dissipation performance and insulation performance, which increases a contact area with the capillary by deformation of the rubber, and thereby, heat dissipation effects increase and the capillary can be prevented from being damaged by a cushion property. A soft member such as a rubber is collapsed and deformed when a load is applied, and a contact area with the capillary decreases, or an air layer is formed to prevent heat conduction, and thereby, a shape and the amount of deformation need to be controlled depending on hardness. 
     The support body  03  of the capillary cartridge according to the present embodiment has a box-shaped structure, and the heat dissipation body  04  is limited so as not to be deformed to a certain size or more in a planar direction by a protrusion portion  03 A which is provided on an outer peripheral portion of the support body  03  and protrudes toward the heat dissipation body  04 . In addition, by providing a gap between an end portion of the heat dissipation body  04  and the outer peripheral portion of the support body  03 , that is, by designing an offset distance to the protrusion portion  03 A of the support body  03  in consideration of an elastic modulus of the heat dissipation body  04 , it is possible to prevent the heat dissipation body  04  from protruding beyond the protrusion portion  03 A to be deformed. In addition, a height of the protrusion portion  03 A of the support body  03  is smaller than a thickness of the heat dissipation body  04 , and thereby, even if a load is applied, the heat dissipation body  04  is not collapsed beyond the height of the support body  03 . Accordingly, it is possible to reliably make the heat dissipation body  04  come into contact with a device surface to which the capillary cartridge is attached. For example, if silicon rubber with thermal conductivity of 0.1 to 5 W/m·K is used for the heat dissipation body  04 , heat dissipation performance of 200 W/m 2 ·K or more can be obtained. Various rubbers other than silicon, an elastomer, a heat dissipation gel, or the like may be used for the heat dissipation body  04 . 
       FIG.  7    illustrates an example of a detailed view of attachment of the capillary cartridge according to the present embodiment. If a detection unit positioning pin  13  is attached to an attachment surface  50  on the thermostatic bath unit  41  side of the electrophoresis apparatus and is pushed through a positioning hole  14  of the support body  03 , the detection unit  06  is temporarily fixed by a clip  52 . At the same time, since a taper-shaped electrode holder positioning pin  15  on the thermostatic bath unit  41  of a device to be attached is automatically inserted into an electrode holder positioning hole  16  of the support body  03 , the capillary cartridge  01  is temporarily fixed to the thermostatic bath unit  41  by a one-time operation. The electrode holder positioning pin  15  and the electrode holder positioning hole  16  may be attached to opposite positions. That is, the electrode holder and the support body can be fixed by making the electrode holder positioning pin provided on one side pass through the electrode holder positioning hole provided on the other side. 
       FIG.  8    illustrates a sectional view of the clip  52 . A structure is provided in which, if the capillary cartridge  01  approaches the attachment surface  50 , and if the detection unit  06  hits a projection portion of the clip  52  so as to push the clip  52  once and further approaches as illustrated in an upper stage of the figure, the detection unit  06  crosses over the projection portion of the clip  52 , the clip  52  presses the detection unit  06  by reaction force of a spring  53 , and the clip  52  temporarily fixes the detection unit  06  as illustrated in an intermediate stage and a lower stage of the figure. At this time, at the same time when the detection unit crosses over the projection portion, the clip  52  is instantaneously moved by the reaction force, and thereby, click sound is made, and a user can confirm that the capillary cartridge  01  is temporarily fixed. 
     In this way, according to the present embodiment, positioning is first performed from the detection unit  06 , and thereby, the detection unit  06  and an optical system of the electrophoresis apparatus can be reliably positioned with high accuracy. In addition, by making the electrode holder positioning pin  15  have a tapered shape as illustrated in  FIG.  7   , even if a location of the electrode holder positioning pin  15  is shifted to some extent for each device, the electrode holder positioning pin is reliably inserted into the electrode holder positioning hole  16 , and thereby, the electrode holder  05  can also be temporarily fixed if a location of the detection unit  06  is determined. Accordingly, since a user can perform a series of an attachment operation of the capillary cartridge  01  with the handle  09  which is a grip portion, it is possible to operate without touching the detection unit  06  with a hand or forcibly bending the capillary  02 , and thereby, usability can be improved and a damage risk can be reduced. 
       FIG.  9    illustrates a detailed view of adjustment margin of the capillary of the capillary cartridge according to the present embodiment. In order to fill the capillary  02  with an electrophoresis medium, the capillary head  07  and the electrophoresis medium container  28  are connected to each other when the capillary cartridge  01  is attached, but, at this time, a location of the capillary head  07  has to be moved according to a location of the electrophoresis medium container  28 . This is because, if heights of the capillary head  07  and a tip portion of the electrode (cathode)  08  are not aligned, a siphon phenomenon occurs in which the electrophoresis medium in the capillary  02  moves, and thereby, the heights need to be aligned with high accuracy. 
     As illustrated in a lower stage of the figure, if a length of the capillary  02  from the detection unit  06  to the capillary head  07  is designed to be the shortest distance to a polymer container such as the electrophoresis medium container  28 , when a location of the polymer container is shifted by a device, the capillary head  07  moves to the left and right, and thereby, the height is also changed. In the present embodiment, as illustrated in an upper stage of the figure, the capillary  02  is designed to have a length obtained by adding the adjustment margin to the shortest distance from the detection unit  06  to the polymer container, and, even if the capillary head  07  moves in the left-right direction, it is possible to maintain as is aligned at the same height as the tip portion of the electrode (cathode)  08 . 
     If the capillary head  07  moves in a state where the capillary cartridge  01  is completely fixed, there is a risk of abrasion of the heat dissipation body  04  or excessive force being applied to the capillary  02 , and thus, the present embodiment provides a structure in which the capillary cartridge  01  is temporarily fixed once before the capillary head  07  is connected. As illustrated in  FIG.  8   , since the detection unit  06  is held in perpendicular direction of the cartridge by the projection portion of the clip  52  and in a planar direction of the cartridge by the reaction force of the spring  53  of the clip  52 , a structure is provided in which the detection unit is not deviated by a tension possessed by the capillary  02  and the capillary cartridge  01  does not come off as a user moves the capillary head  07 . 
     Then, after the capillary head  07  is connected to the electrophoresis medium container  28 , the capillary cartridge  01  is pressed by the thermostatic bath door  43  illustrated in  FIG.  1   , and thereby, the detection unit  06  is fixed by pushing the clip  52 . At the same time, the electrode holder positioning pin  15  is deeply inserted into the electrode holder positioning hole  16 , and thereby the entire capillary cartridge  01  is completely fixed. At this time, as the detection unit positioning pin  13  is deeply inserted, a click sound is made and thus, a user can confirm that the capillary cartridge  01  is completely fixed. The click sound may be made by using the same structure as the clip  52  for a fixing portion of the electrode holder  05 . 
       FIG.  10    illustrates a work flow of attachment of the capillary cartridge  01  according to the present embodiment. First, the detection unit  06  is temporarily fixed, and at the same time, the electrode holder  05  is temporarily fixed (S 101 ). Next, the capillary head  07  and the electrophoresis medium container  28  are connected to each other (S 102 ), and finally, the thermostatic bath door  43  is closed, and thereby, the capillary cartridge  01  is pushed in and fixed (S 103 ) and the attachment is completed (S 104 ). As such, by fixing one place, the entire structure is also fixed automatically, a procedure can be reduced, and complexity of attachment of the capillary  02  can be reduced. 
       FIG.  11    illustrates a sectional view of a peripheral portion of the capillary  02  according to the present embodiment. If, for example, silicone rubber is used as the heat dissipation body  04 , when a predetermined load is applied by making the capillary cartridge  01  come into contact with the attachment surface  50  on the electrophoresis apparatus side, the heat dissipation body  04  is deformed along a shape of the capillary  02 , and thereby, a contact area with the capillary  02  can be increased. At this time, a uniform load is uniformly applied to the entire surface of the heat dissipation body  04  by a door, and thereby, it is difficult to form an air layer between the attachment surface  50  and the heat dissipation body  04 . In contrast to this, if the air layer completely disappears, there is a possibility that the heat dissipation body  04  becomes like a suction cup and the capillary cartridge  01  cannot be detached. In the present embodiment, an integral structure having the capillary  02  interposed therebetween is provided, the capillary  02  is disposed to an external side of the heat dissipation body  04 , and the air layer does not completely disappear. Accordingly, it is possible to simply detach without sticking to the attachment surface  50 . 
       FIG.  12    illustrates a structure example of a thermostatic bath door according to the present embodiment, and the thermostatic bath door has a two-stage structure having an elastic body such as a spring interposed therebetween. That is, the thermostatic bath door  43  has a two-stage structure in which a push plate  57  is attached to a door support body  58  through one or more push plate spring  56 , and has a cushion property. By adjusting a spring constant, it is possible to control the load applied to the capillary cartridge  01  when the thermostatic bath door  43  is closed. For example, if twelve springs  53  with a spring constant of 3 N/mm are used, a load of 30 N can be applied when the thermostatic bath door  43  is closed. 
     As a heat dissipation body between a capillary including a detection unit provided in a part thereof and a plate-shaped support body for supporting the capillary is provided by the capillary cartridge according to embodiment 1 described in detail above, temperature increase inside the capillary can be suppressed by the heat dissipation body, and thereby, it is possible to perform electrophoresis under a high voltage application condition where the amount of heat increases and to reduce the analysis time. In addition, it is possible to improve complexity of an operation by reducing the number of fixing places at the time of attachment by adopting a structure in which the capillary, the support body, and the heat dissipation body are integrated. 
     Furthermore, in the present embodiment, an integral structure is provided in which the detection unit and the cathode electrode portion of the capillary are held in the support body, two places of the support body and the anode electrode portion of the capillary are fixed and the entire capillary cartridge is pushed to a door mechanism of the electrophoresis apparatus to be fixed thereto, and thereby, it is possible to easily attach with few procedures. In addition, furthermore, since the disposition of the capillary is also supported, it is also possible to reduce the risk of damage. 
     Furthermore, in the present embodiment, since the capillary is in direct contact with a member with high thermal conductivity, heat generated from the capillary can be dissipated by applying a high voltage at the time of electrophoresis. Thereby, since temperature inside the capillary is stabilized at a predetermined temperature, it is possible to improve analysis performance of the electrophoresis apparatus and to reduce the analysis time. 
     Embodiment 2 
     Example 2 is an embodiment of a capillary cartridge in which a shape of the heat dissipation body of the capillary cartridge is a planar shape corresponding to a region where the capillary creeps on the support body. As illustrated in  FIG.  13   , while the minimum amount of heat to dissipate the heat of the capillary is maintained, a region of the heat dissipation body  04  is limited so as to conform to the shape of the capillary, and thereby, it is possible to reduce a cost of the capillary cartridge while the heat dissipation performance is maintained. For example, it is possible to obtain a heat dissipation performance of 200 W/m 2 ·K or more, even in a state where the heat dissipation body  04  is disposed only in the peripheral portion of the capillary  02  illustrated in  FIG.  5    and the amount of heat is reduced to 50 J/K. Also in the present figure, since the heat dissipation body  04  is disposed on the back side of the support body  03 , the heat dissipation body is indicated by a dotted frame. The heat dissipation body  04  may be attached to the support body  03  by joining a sheet of simple shape such as a rectangle. Furthermore, a shape of the support body  03  may be smaller in accordance with a shape of the heat dissipation body  04 , such that the cost can be further reduced. 
     Embodiment 3 
     Embodiment 3 is an embodiment of a capillary cartridge configured to separately fix a detection unit and an electrode holder. The electrode holder is fixed by making an electrode holder positioning pin on an apparatus side pass through an electrode holder positioning hole as in the first embodiment. Meanwhile, as illustrated in  FIG.  14   , the detection unit  06  is configured to be connected to the support body  03  by an S-shaped detection unit holding member  54 , and thereby, a location can be flexibly moved, matched, and fixed in a planar view. According to the present embodiment, an attaching operation can be performed more simply. 
     Embodiment 4 
     Embodiment 4 is an embodiment of providing a hand holding hole functioning as a grip portion is provided in the support body instead of a handle of the capillary cartridge. As illustrated in  FIG.  15   , as a user inserts his/her finger into a hand holding hole  55  of the support body  03  and holds to operate the capillary cartridge  01 , it is possible to reduce a risk of dropping at the time of carrying or attaching. In addition, by disposing the hand holding hole  55  near the detection unit, positioning can be easily performed. Alternatively, by disposing the hand holding hole  55  in a well-balanced state near the center portion, the force applied at the time of detachment is dispersed without concentrating at one place, deformation or distortion of the support body  03  can be prevented, and efficiency of attachment work and detachment work can be increased. 
     Embodiment 5 
     Embodiment 5 is an embodiment in which the support body of the capillary cartridge has a two-stage structure. As illustrated in  FIG.  16   , the two-stage structure is provided in which a push plate  57  is attached to the back side of the support body  03  to which the heat dissipation body  04  is attached and one or more push plate springs  56  are inserted between the support body  03  and the push plate  57 , the capillary cartridge itself can have a cushion property. In a case of the present embodiment, there is no need to form the thermostatic bath door  43  described in Embodiment 1 as the two-stage structure. 
     As described in detail above, according to the present invention, since temperature increase inside the capillary can be suppressed by the heat dissipation body, it is possible to perform electrophoresis under a high voltage application condition where the amount of heat increases and to reduce the analysis time. In addition, it is possible to redress complexity of an operation by reducing the number of fixing places at the time of attachment by adopting a structure in which the capillary and the support body are integrated. Thereby, it is possible to achieve both improvement of analysis performance and improvement of usability. 
     The present invention is not limited to the above-described embodiments, and includes various modification examples. For example, the above-described embodiments are described in detail for a better understanding of the present invention, and are not necessarily limited to those having all the configurations of the description. In addition, it is possible to perform addition, deletion, and replacement of other configurations with respect to a part of a configuration of each embodiment. For example, it is also possible to form a detection unit positioning hole on an attachment surface on a thermostatic bath unit side of an electrophoresis apparatus, and to provide a detection unit positioning pin pushing structure formed in the support body. In addition, it is possible to replace a part of a configuration of one embodiment with a configuration of another embodiment, and to add a configuration of another embodiment to a configuration of one embodiment. 
     REFERENCE SIGNS LIST 
       01 : capillary cartridge,  02 : capillary,  03 : support body,  04 : heat dissipation body,  05 : electrode holder,  06 : detection unit,  07 : capillary head,  08 : electrode (cathode),  09 : handle,  10 : electrode holder fixing pin,  11 : electrode holder fixing hole,  12 : detection unit fixing frame,  13 : detection unit positioning pin,  14 : positioning hole,  15 : electrode holder positioning pin,  16 : electrode holder positioning hole,  20 : autosampler unit,  21 : sampler base,  22 : X-axis drive body,  23 : Y-axis drive body,  24 : Z-axis drive body,  25 : sample tray,  26 : sample container,  27 : liquid feeding mechanism,  28 : electrophoresis medium container,  29 : anode side buffer liquid container,  30 : anode side wash layer,  31 : anode side electrophoresis buffer liquid layer,  32 : anode side sample introduction buffer liquid layer,  33 : cathode side buffer liquid container,  34 : waste liquid layer,  35 : cathode side wash layer,  36 : cathode side electrophoresis buffer liquid layer,  40 : irradiation detection/thermostatic bath unit,  41 : thermostatic bath unit  42 : irradiation detection unit,  43 : thermostatic bath door,  44 : electrode (anode),  50 : attachment surface,  52 : clip,  53 : spring,  54 : detection unit holding member,  55 : hand holding hole,  56 : push plate spring,  57 : push plate,  58 : door support body