Patent Publication Number: US-2003235837-A1

Title: High resolution typing system for pathogenic E. coli

Description:
CROSS REFERENCE  
     [0001] This application claims priority from U.S. Provisional Patent Application Serial No. 60/339,687, filed Dec. 11, 2001, the disclosure of which is incorporated by reference herein in its entirety. 
    
    
     
       FIELD OF INVENTION  
       [0002] This invention relates generally to molecular sub-typing of bacteria by genetic analysis of variable number tandem repeat (VNTR) sequences. More specifically, the invention is directed to a system for DNA sub-typing of pathogenic  E. coli  by multiple-locus variable number tandem repeat analysis (MLVA) and to an 15 epidemiological database constructed 15 from data generated by the system.  
       BACKGROUND  
       [0003] In the light of recent meat-related food scares, public concern about food safety caused by  E coli  contamination continues to escalate. Disease incidence associated with the O157:H7 pathogenic strains has been on the rise since the 1980s when it was first associated with hemorrhagic colitis (18). Many outbreaks have been described subsequently worldwide, but in the United States it is well known for its appearance in a Washington State fast food chain outbreak (9). Since this infamous media introduction to the public, it has been associated with several very large packaged meat recalls (3, 4), outbreaks in daycare centers (1) and has even been associated with water-borne infections at an amusement park (20, 5, 12).  
       [0004] Crucial epidemiological links between outbreaks of  E. coli  infections at disparate times and places would help prevent the spread of disease. The challenge is to identify the strain and correlate it with source. Molecular typing has long been a part of pathogen identification and control. Traditionally, serotyping has been used to identify important cellular components associated with virulence. Newer approaches include multilocus enzyme electrophoresis, DNA typing, and ribotyping. Comparative gene sequencing such as multiple-locus sequence typing (MLST) has been used to distinguish among both species and strains and is useful in subtyping those bacteria presenting sufficient nucleotide diversity.  
       [0005] Currently, the most widespread approach to subtyping enteric pathogens is pulse field gel electrophoresis (PFGE) detection of restriction fragment length polymorphisms (19). Pulsed-field gel electrophoresis (PFGE) can resolve very large and sometimes polymorphic DNA restriction fragments. PFGE data is currently generated by hundreds of laboratories across the U.S. that contribute to PulseNet, a database established for epidemiological monitoring of outbreaks (19). However, PFGE is a cumbersome technology that cannot easily handle very large sample sets. Nor is PFGE data well suited for database comparisons due to the continuous nature of fragment sizes and has limited discrimination capacity for closely related isolates. Moreover, PFGE data sets are not easily standardized for transfer throughout the public health community. Yet PFGE is a “universal” technology that works on any bacteria without requiring prior genomic information for primer design. For at least the near future, the PFGE advantages and large integrated user community deem this as the technology of choice. However, more highly discriminating subtyping methods have been sought to offer complementary analysis approaches.  
       [0006] Polymerization chain reactions (PCR) methods offer many technical advantages over the PFGE technique. PCR detects a small amount of a specific DNA sequence by amplifying it to levels that can be readily observed. PCR-based methods have become increasingly important to molecular typing efforts. These approaches include amplified fragment length polymorphism (AFLP), repetitive element polymorphisms-PCR, randomly amplified polymorphic DNA, and arbitrarily primed PCR The power of PCR-based methods is the ease with which they can be applied to many bacterial pathogens and their multilocus discrimination. However analysis of an entire genome is not possible with current PCR instruments. More discriminatory methods to provide molecular sub-typing have been sought.  
       [0007] It has recently been discovered that small polymorphic genomic regions, termed variable number tandem repeat VNTR sequences, provide a sensitive and reliable basis for molecular typing (7, 14, 15). VNTR are present in the genomes of most bacteria including  E. coli.  Many allelic states are observed in the VNTR among diverse strains and characteristic recurrence patterns are the basis of subtyping and identifying the strains. VNTR loci appear to be among the most diverse in bacterial genomes (21). As a result VNTRs appear to contain greater diversity and, hence, greater discriminatory capacity than any other type of molecular typing system.  
       [0008] The speed and efficiency of sub-typing bacteria is improved in multiple-locus VNTR analysis (MLVA). In MLVA, multiple markers are used to discriminate between related bacterial strains in a number of different isolates (Keim anthrax paper). U.S. Pat. No. 6,479,235 describes a multiplex technique that may be used for rapid, simultaneous analysis of DNA in multiple loci. MLVA may be used to resolve otherwise indistinguishable strain types and to phylogenetically define them relative to other close isolates (14, 15, 7).  
       [0009] DNA subtyping of the  E. coli  O157:H7 pathogenic strains by a MLVA system is a useful epidemiological approach. Because of the highly monomorphic molecular nature of  E. coli,  MLVA may be the only reasonable method with which to study the diversity, evolution, and molecular epidemiology of this pathogen. However, MLVA analysis requires identification of suitable marker DNA in the bacteria of interest and requires specific primers for amplifying the marker DNA.  
       SUMMARY  
       [0010] Variable number tandem repeat (VNTR) sequences have been identified in the genome of certain  E. coli  strains. It has been discovered that the VNTR sequences exhibit length polymorphism at different loci. A sub-typing system based on multiloci size analysis of VNTR is the basis of the novel molecular sub-typing system of the present invention.  
       [0011] A molecular typing system is provided wherein VNTR sequences at a number of loci in an  E. coli  DNA sample are analyzed simultaneously and then evaluated for size. Discrete data is thereby generated that is characteristic of each sub-strain.  
       [0012] The small size of the VNTR relative to the whole genomic DNA makes it difficult to observe the sequences directly in a DNA sample with current technology. However, PCR methods are well known and may be used to amplify small loci containing the VNTR to amounts sufficient for size evaluation.  
       [0013] Accordingly, in preferred embodiments of the present invention, a molecular typing system is provided wherein multiloci containing VNTR are simultaneously amplified by PCR, preferably multiplex, and then separated by size. Size separation is preferably by gel or capillary electrophoresis. Tagged primers designed for each locus containing a VNTR sequence allow discrimination of loci and assignment of each amplified VNTR sequence to a genomic allele.  
       [0014] In an important aspect of the present invention, primers are provided for amplifying  E. coli  loci comprising VNTR. A representative sample of primers for amplifying loci from  E. coli  containing VNTR sequences include, but are not limited to the following primer pairs: SEQ ID No. and SEQ ID NO;  
       [0015] For use in the present sub-typing system, the primers comprise an observable indicator whereby an amplified loci containing a VNTR sequence may be identified, Preferably the indicator is a colored dye attached to one member of a primer pair, most preferably a fluorescent dye selected from the group consisting of HEX,  
       [0016] In certain embodiments of the present invention, multiplex cocktails containing two or more primers are provided for simultaneous amplification of multiloci containing VNTR in sample  E. coli  DNA. Suitable multiplex cocktails are exemplified by, but not limited to, the following primer sets having SEQ ID NO . . . .  
       [0017] In an important aspect of the present invention, kits are provided for use in sub-typing  E. coli  by PCR. In certain preferred embodiments, the kits comprise primers designed for  E. coli  loci containing a VNTR sequence. In certain other preferred embodiments, the kits comprise multiplex cocktails. The kits also comprise amplifying reagents for creating amplification conditions during an analysis in a PCR instrument. Generally the amplifying reagents comprise a polymerase, preferably taq polymerase, dntp selected from ATP, GTP, CTP and TPS and suitable salts and buffers to maintain amplification reaction conditions. In certain instances the kits may also comprise reference sample DNA. In certain other instances, the kits may comprise reagents and materials for allowing size separation and analysis of amplified products.  
       [0018] In yet another important aspect of the present invention, methods are provided for sub-typing an  E. coli  strain using PCR comprising the steps of:  
       [0019] (a) obtaining one or more primers specific for loci in an  E. coli  strain comprising VNTR sequence, said primer pair having an observable indicator,  
       [0020] (b) obtaining single-stranded sample DNA from the  E. coli  sample to be subtyped;  
       [0021] (c) combining said primers, said sample DNA and amplifying reagents under hybridizing and amplifying conditions in a PCR instrument to form amplicons comprising said primers and said marker DNA,  
       [0022] (d) separating the amplicons by size;  
       [0023] (e) evaluating the loci by observing the indicator in the separated amplicons and  
       [0024] (e) comparing said evaluation to an evaluation of a standard  E. coli  strain.  
       [0025] In an important aspect of the present invention, the VNTR sequences of the present invention are provided as a research tool for identifying novel molecular species, especially proteins, produced by the variable VNTR sequences present in rapidly evolving  E. coli  strains.  
       [0026] In yet another important aspect of the present invention, a method of producing discrete genetic data for an epidemiological database is provided. The data generated in the molecular sub-typing system of the present invention is in the form of discrete integral numbers about VNTR size and allelic location. A database containing this discrete information may be constructed worldwide over a long period of time. The database will be a powerful tool for containing the spread of disease. 
     
    
    
     BRIEF DESCRIPTIONS OF THE FIGURES  
     [0027]FIG. 1. is a histogram of tandem repeats located in three  E. coli  genomes . . . .  
     [0028]FIG. 2. illustrates the alleles and diversity value for 30 polymorphic VNTR loci in 56  E. coli  isolates.  
     [0029]FIG. 3 presents the genetic relationships among 56  E. coli  isolates in a neighbor joining tree based upon analysis of 30 VNTR marker loci described in the present invention.  
     [0030]FIG. 4 is a photograph of an electrophoresis slab gel showing the separation pattern of amplified marker DNA from  E. coli  by size and colored primer. An  E. coli  strain may by sub-typed by comparing the pattern obtained in the gel with the pattern obtained with an  E. coli  strain of known type. 
    
    
     DETAILS OF THE INVENTION  
     [0031] In one aspect, the present invention provides a molecular sub-typing system for  E. coli  based on analysis of VNTR loci. VNTR loci consist of short, repetitive sequence elements of a number of base pairs. Variation in the number of repeat units at a particular locus is responsible for the observed polymorphism observed at VNTR loci and is the basis of the present sub-typing system. Repeat arrays in an unknown  E. coli  strain are observed and compared to known strains. The VNTR locus repeat-size is easily defined, allowing the designation of specific alleles in a discrete, rather than continuous, data set. This is a great advantage for database results generated from multiple laboratories across several years.  
     [0032] VNTR loci have been identified in two  E. coli  whole genomic sequences and used to subtype  E. coli  O157:H7 strains. A representative sample of VNTR loci sequences according to the present invention include, but are not limited to SEQ ID numbers. 0163 to 0320, inclusive. It is to be understood that certain substitutions in these sequences occur naturally, but such substitutions do preclude the functionality of the VNTR loci for use in the present sub-typing system. Accordingly, VNTR loci functionally equivalent to the SEQ ID numbers 0163 to 0320 are intended to be included as members of the group.  
     [0033] It is anticipated that VNTR loci will be discovered in other  E. coli  and eventually will provide the basis for a global  E. coli  molecular sub-typing system. The present description is intended to provide details of a sub-typing system for  E. coli  O157:H7 that is exemplary of the system to be used for sub-typing other  E. coli  strains.  
     [0034] In a preferred embodiment of the present invention, the molecular sub-typing system comprises,  
     [0035] (a) primers for amplifying VNTR loci from  E. coli  DNA sample, said primers including an observable indicator;  
     [0036] (b) means for amplifying said primer and VNTR loci DNA to form amplicons;  
     [0037] (c) means for size-separating amplicons  
     [0038] (d) means for observing the indicator on said separated amplicons and  
     [0039] (e) means for calculating the VNTR repeat array in the  E. coli  DNA.  
     [0040] In an important aspect, primers are presented for amplifying VNTR loci from  E. coli  O157:H7 in PCR, preferably multiplex. A representative sample of primers that have been designed around these loci to amplify VNTR loci sequences according to the present invention include, but are not limited to SEQ ID NO&#39;s 0001 to 0162.inclusive.  
     [0041] In operation these primers are used in pairs selected from the group:  
     [0042] SEQ ID No. 0011+0013,  
     [0043] SEQ ID No. 0103+0105,  
     [0044] 0035+0037,  
     [0045] 0039+0043,  
     [0046] 0091+0093,  
     [0047] 0099+0101,  
     [0048] 0115+0117,  
     [0049] 0023+0025  
     [0050] 0019+0021,  
     [0051] 0053+0055,  
     [0052] 0127+0129,  
     [0053] 0107+0109,  
     [0054] 0027+0029,  
     [0055] 0073+0075,  
     [0056] 0015+0017,  
     [0057] 0083+0085,  
     [0058] 0069+0071,  
     [0059] 0047+0051,  
     [0060] 0077+0079,  
     [0061] 0111+0113,  
     [0062] 0119+0121,  
     [0063] 0065+0067,  
     [0064] 0007+0009,  
     [0065] 0087+0089,  
     [0066] 0123+0125,  
     [0067] 0139+0141,  
     [0068] 0159+0161,  
     [0069] 0057+0061,  
     [0070] 0001+0003,  
     [0071] 0031+0033,  
     [0072] 0095+0097,  
     [0073] 0131+0133,  
     [0074] 0135+0137,  
     [0075] 143+0145,  
     [0076] 0147+0149,  
     [0077] 0151+0153, and  
     [0078] 0155+0157.  
     [0079] In the present method, amplification of the VNTR loci from  E. coli  results in amplicons comprising DNA of the primer pairs and the VNTR loci. Representative of amplicons comprising primers and VNTR loci from  E. coli  O157:H7 selected from the group consisting of SEQ ID NO. 0321 to SEQ ID NO. 0478 inclusive.  
     [0080] For use in sub-typing  E. coli,  the primers have an observable indicator. Preferably, the indicator is a dye attached to the primer. When amplified, the dye is incorporated into the amplicon and, after size separation of the amplicons, indicates the VNTR locus of each of the separated amplicons. The allelic array of VNTR is thus associated with discrete data that is characteristic of each  E. coli  strain and allows identification of strains.  
     [0081] Fluorescent dyes in commercial use are suitable indicators for use as indicators in the present sub-typing system. Preferred fluorescent dyes are HEX, FAM, NED, ROX available from Applied Biosystems (Foster City, Calif.) and dyes supplied by Beckman, Inc. (Fullerton, Calif.).  
     [0082] Preferred embodiments of the present invention are directed to MLVA methods of simultaneously analyzing multiple VNTR loci sequences. In preferred embodiments of the present invention, the PCF technique termed “multiplex” amplification methods are employed. In this technique, multiplex cocktails containing two or more labeled primer pairs are prepared and used for determining multiple VNTR loci in a sample DNA simultaneously. This technique generates large amount of data from a single amplification and thus provides efficiency and cost savings without loss of discriminatory power.  
     [0083] Multiplex cocktails for sub-typing  E. coli  O157:H7 are presented. The cocktails comprise primer sets selected from the group consisting of:  
     [0084] Set number one containing primers SEQ ID No. 0011 and 0013, SEQ ID No 0103 and 0105, SEQ ID No 0035 and 0037, SEQ ID No 0039 and 0043;  
     [0085] Set number two containing primers having seq. ID No.0091 and 0093, 0099 and 0101, 0115 and 0117, 0023 and 0025, 0019 and 0021;  
     [0086] Set number three having Seq. ID No 0053+0055, 0127+0129, 0107+0109, 0027+0029, 0073+0075, 0015+0017;  
     [0087] Set number four D No 0083+0085, 0069+0071, 0047+0051, 0077+0079, 0111+0113  
     [0088] Set number five Seq. ID No 0119+0121, 0065+0067, 0007+0009, 0087+0089, 0123+0125, 0139+0141; and  
     [0089] Set number six containing primers Seq. ID No 0159+0161, 0057+0061, 0001+0003  
     [0090] In an important aspect of the present invention, kits are provided for supplying sub-typing reagents needed to amplify VNTR loci in a PCR instrument. The kits supply primers or sets of primers for VNTR loci in the bacteria of interest. The kits also supply the necessary reagents for creating the hybridization and amplification conditions during a PCR run. Preferably the reagents comprise an amplifying agent, most preferably taq polymerase, dntp as building blocks, and salts and buffers for the reactions. The commercial availability of kits will encourage the development and use of the present  E, coli  molecular system. The ease of use of the kits and the increasing simplicity of the PCR technique will allow researchers and clinicians in even remote parts of the world to analyze infectious strains by the present sub-typing system. This will improve the containment of disease at the point of outbreak worldwide.  
     [0091] In a preferred embodiment of this aspect, kits are provided for sub-typing  E. coli  for use in PCR amplifications. In certain instances kits for sub-typing  E. coli  O157:H7 are. These kits comprise primers of the present invention for amplifying VNTR loci from this strain by PCR. In other preferred embodiments, kits comprise multiplex cocktails as described hereinabove are provided for multiplexing.  
     [0092] In an important aspect of the present invention methods are provided for sub-typing  E. coli.  The method comprises the steps of:  
     [0093] (a) obtaining one or more primers for amplifying loci comprising VNTR said primers having an observable indicator,  
     [0094] (b) obtaining single-stranded sample DNA from the  E. coli  sample to be subtyped;  
     [0095] (c) combining said primers, said sample DNA and amplifying reagents under hybridizing and amplifying conditions in a PCR instrument to form amplicons comprising said primers and said VNTR;  
     [0096] (d) separating the amplicons by size;  
     [0097] (e) evaluating numbers and sizes of separated amplicons and  
     [0098] (e) comparing said evaluation to an evaluation of amplicons obtained by PCR from a known  E. coli  strain.  
     [0099] The method may be modified for sub-typing a strain of interest by employing a primer specific for VNTR loci identified in known strains. In preferred embodiments the method may be used to sub-type  E. coli  O157:H7 by using the primers having sequence ID NO 0163 to 0320, inclusive. Any primers or multiplex cocktails capable of use for amplifying the VNTR loci having SEQ ID numbers. 0163 to 0320, inclusive may be may be used in the present method.  
     [0100] In preferred embodiments of the invention, amplicons are size-separated by gel electrophoresis or capillary electrophoresis.  
     [0101] In yet another important aspect of the invention, the sub-typing method may be used to produce discrete genetic data for an epidemiological database. The method generates information concerning VNTR arrays in certain alleles of  E. coli.  This data is provided in the form of discrete numbers that can be compared to numbers generated from analysis of known  E. coli  strains and sub-strains. A database of known strains will be compiled and identification of unknown strains from clinical isolates is made possible by comparison to known strains. The epidemiological value of this database for global control of diseases caused by bacterial infection is profound.  
     [0102] In yet another important aspect of the present invention, certain VNTR loci sequences are provided for use as research tools. It is known that certain  E. coli  strains are rapidly evolving and this is reflected in the variable polymorphism of the VNTR loci. The methods and means of the present invention may be used to identify and amplify these loci to study the molecules expressed.  
     [0103] The present invention may be better understood with reference to the accompanying examples that are intended for purposes of illustration only and should not be construed to limit the scope of the invention, as defined by the claims appended hereto.  
     EXAMPLES  
     Example 1  
     [0104] This Example illustrates the method of the present invention for molecular sub-typing of a sample DNA by multiplex.  
     [0105] DNA was prepared from a single colony of a pure culture as a simple whole-cell heat lysate from a single colony. This involves boiling a colony of  E. coli  in Tris-EDTA for 20 min and then removing the cellular debris through centrifugation. The remaining liquid contains a crude DNA extract that is suitable for use in this system.  
     [0106] All reagents used for PCR were obtained from Life Technologies, unless otherwise noted. PCR conditions for all mixes use 1U Platinum Taq, 1X PCR buffer, 2 mM MgCl 2  and 0.2 mM dNTPs final concentration in a total reaction volume of 10 ul. Primer concentrations for each multiplex mix are as follows: Mix 1 has primers pairs with Seq. ID No. 0011/0013, 0103/0105, 0035/0037, and 0039/0043 at final concentrations of 0.1, 0.6, 0.2, and 0.3 mM respectively; Mix 2 has primers pairs with Seq. ID No. 0091/0093, 0099/0101, 0115/0117, 0023/0025 and 0019/0021 at final concentrations of 0.05, 0.1, 0.1, 0.5, and 0.4 mM respectively; Mix 3 has primers pairs with Seq. ID No 0053/0055, 0127/0129, 0107/0109, 0027/0029, 0073/0075, and 0015/0017 at final concentrations of 0.1, 0.2, 0.1, 0.4, 0.05, and 0.3 mM respectively; Mix 4 has primers pairs with Seq. ID No 0083/0085, 0069/0071, 0047/0051, 0077/0079, and 0111/0113 at final concentrations of 0.1, 0.3, 0.2, 0.4, and 0.1 mM respectively; Mix 5 has primers pairs with Seq. ID No 0119/0121, 0065/0067, 0007/0009, 0087/0089, 0123/0125, and 0139/0141 at final concentrations of 0.2, 0.3, 0.2, 0.1, 0.1, and 0.6 mM respectively; and Mix 6 has primers pairs with Seq. ID No 0159/0161, 0057/0061, 0001/0003 at final concentrations of 0.2, 0.05, and 0.6 mM respectively. The remaining primers pairs with Seq. ID No, 0031/0033, 0095/0097, 0131/0133, 0135/0137, 143/0145, 0147/0149, 0151/0153, and 0155/0157 are not currently multiplexed, but are run under identical conditions to the above multiplex mixes with the exception that a final concentration of 0.2 mM is used for each primer. Future plans include incorporating these final markers into the existing multiplexes. To each 9 ul of master mix for the PCR reaction, 1 ul of a {fraction (1/10)} dilution of the heat lysate DNA was added. Thermocycling parameters raised the PCR mixtures to an initial temperature of 94° C. for 5 min, with cycling of 94° C. for 20 sec, 65° C. for 20 sec, and 72° C. for 20 sec a total of 35 times with a final extension step of 72° C. for 5 min. PCR products were diluted five-fold prior to combining equally with ROX-labeled Map Marker 1000 (BioVentures, Inc.) custom size standard. Fluorescently labeled PCR product was visualized using polyacrylamide gel electrophoresis on a Perkin-Elmer Applied Biosystems 377 DNA sequencer. Fragment sizing was performed using GeneScan and Genotyper analysis software (Perkin-Elmer, ABI).  
     Example 2  
     [0107] This Example illustrates the detection of VNTR sequences useful for sub-typing.  
     [0108] Tandem repeat structures were detected in the completed genomes of the K-12, EDL933 O157:H7, Sakai O157:H7 and in plasmids pO157 and pOSAK1 sequences obtained from the NCBI genome website. Repeats were found with the use of two software programs. Small (1 to 10 bp motif) perfect repeats were detected using SSR Finder (Gur-Arie et. al. 2000). Larger (&gt;8 bp) perfect and imperfect repeats were found using GeneQuest (DNAstar software; LaserGene, Inc., Madison, Wis.). This program was also used to preliminarily determine if arrays were located in an ORF, while final confirmation was made by blasting the sequences against the annotated genome at the NCBI website server. Primers were designed around potential VNTRs using PrimerSelect (DNAstar software) or Oligo (ver. 6.52, Molecular Biology Insights, Inc.). Primers were designed with a Tm range of 68 to 72° C.  
     [0109] As illustrated in FIG. 1, there are thousands of potential VNTR loci in the  E. coli  genome. 67 potential VNTR loci were selected based upon the repeat size and copy number and were screened by PCR to maximize discrimination power and suitability for an electrophoretic assay. Of these, 37 generated robust PCR amplification and exhibited significant size variations strains. FIG. 2 illustrates the Locus O157-39 (#) was monomorphic, but useful as a presence-absence diagnostic marker for pOSAK1. Markers that contain a null-state allele in addition to fragment size variation are indicated with an asterisk (*). The three markers indicated with striped bars are located on plasmids.  
     [0110] These potential VNTR loci were screened for variability against 56  E. coli  O157:H7/HN and O55:H7 strains (Table 1). Of the original 67 primer sets, 37 were chosen for use in the final analysis (Table 2).  
     Example 3  
     [0111] This Example illustrates a scenario wherein the  E. coli  sub-typing system of the present invention allows rapid identification and containment of an infectious outbreak.  
     [0112] A food borne disease outbreak has occurred where food has been contaminated with pathogenic  E. coli  O157:H7. Public health, law enforcement or other agencies have provided the diagnostic laboratory a clinical  E. coli  isolate from a disease victim who ate the contaminated food. They would like to determine if the victim&#39;s bacterial isolate is the same subtype as is found in the contaminated food and an  E. coli  isolate from a particular food processing plant, or restaurant. Live cultures of each are provided to the diagnostic laboratory. A small portion of each culture is mixed with a small amount of an aqueous buffer and boiled for 10 to 20 minutes. This culture lysate is used as a source of DNA for PCR analysis of multiple variable number tandem repeat (VNTR) loci. A kit is provided containing primers and necessary amplification reagents. After reaction, the PCR products (amplicons) are separated by size via electrophoresis and detected by virtue of a fluorescent dye attached to one primer for each locus-specific primer pair. The number of sequence repeats at multiple VNTR loci is determined by estimation of the PCR amplicon size. These sizes represent a multiple locus genotype that will be compared to a standardized database of known strain genotypes and to the possible contamination source in the food processing plant, or restaurant. A positive strain identification will permit the plant or restaurant to remove the source of contaminated food and thus contain the spread of disease.  
               TABLE 1                          Identification of  E. coli  Isolates       Analyzed by Multiplex PCR with the       Primers of the present invention.                                 ID   Originating                   Source   Lab   Serotype   Origin                         O157:H7/HN Isolates                                  35150   ATCC   O157:H7   no data   no data        43888   ATCC   O157:H7   no data   no data        43890   ATCC   O157:H7   USA-CA   no data        43895   ATCC   O157:H7   USA-OR   hamburger        43894   ATCC   O157:H7   USA-MI   human feces       700378   ATCC   O157:HN   no data   human feces       700927   ATCC   O157:H7   derived from                   ATCC   43895       H6436   CDC   O157:H7   USA-WI   human       H6437   CDC   O157:H7   USA-WI   taco meat       F7349   CDC   O157:H7   USA-GA   human       F7351   CDC   O157:H7   USA-GA   human       F6751   CDC   O157:H7   USA-NY   no data       F6750   CDC   O157:H7   USA-NY   no data       H2495   CDC   O157:H7   USA-CT   Apple Cider       H2498   CDC   O157:H7   USA-CT   Apple Cider       G5244   CDC   O157:H7   Japan-Sakai   no data       H1949   CDC   O157:H7   USA-WA   restaurant standard       01A6720   CA Dept   O157:H7   USA-CA   human           Hlth       01A6819   CA Dept   O157:H7   USA-CA   human           Hlth       01A6820   CA Dept   O157:H7   USA-CA   human           Hlth       01A6910   CA Dept   O157:H7   USA-CA   human           Hlth       01A7050   CA Dept   O157:H7   USA-CA   human           Hlth       01A7412   CA Dept   O157:H7   USA-CA   human           Hlth       01A7146   CA Dept   O157:H7   USA-CA   human           Hlth       01A7396   CA Dept   O157:H7   USA-CA   human           Hlth       01A7408   CA Dept   O157:H7   USA-CA   human           Hlth       01A7414   CA Dept   O157:H7   USA-CA   human           Hlth       01A7457   CA Dept   O157:H7   USA-CA   human           Hlth       01A7458   CA Dept   O157:H7   USA-CA   human           Hlth       EHEC1 1   STEC   O157:H7   USA-OR   hamburger           Center*       EHEC1 5   STEC Center   O157:H7   USA-WA   human       EHEC1 6   STEC Center   O157:H7   Japan-   human                   Okayama       EHEC1 7   STEC Center   O157:H7   USA-WA   human       EHEC1 8   STEC Center   O157:H7   USA-CA   human       EHEC1 9   STEC Center   O157:HN   Germany   human (child)       EHEC1 10   STEC Center   O157:HN   no data   human       EHEC1 11   STEC Center   O157:H7   USA-WA   human       DEC3A   STEC Center   O157:H7   USA-WA   human       DEC3B   STEC Center   O157:H7   USA-WA   human       DEC3C   STEC Center   O157:H7   USA-NM   human       DEC3D   STEC Center   O157:H7   USA-MI   human       DEC3E   STEC Center   O157:H7   Canada   human       DEC4A   STEC Center   O157:H7   Argentina   calf       DEC4B   STEC Center   O157:H7   Denmark   human       DEC4C   STEC Center   O157:H7   Egypt   buffalo       DEC4D   STEC Center   O157:H7   Japan   calf       DEC4E   STEC Center   O157:H7   Denmark   human                 O55:H7 Isolates                                 EHEC1 2   STEC Center   O55:H7   USA-WA   human       EHEC1 3   STEC Center   O55:H7   Sri Lanka   human       EHEC1 4   STEC Center   O55:H7   USA-MI   human       EHEC1 12   STEC Center   O55:H7   no data   meat       DEC5A   STEC Center   O55:H7   USA-NY   human       DEC5B   STEC Center   O55:H7   USA-FL   human       DEC5C   STEC Center   O55:H7   USA-NJ   human       DEC5D   STEC Center   O55:H7   Sri Lanka   human       DEC5E   STEC Center   O55:H7   Iran   human                          
 
     [0113]               TABLE 2                       VNTR Locus PCR Primers.                                            Marker 1     Dye   Forward Primer (5′ to 3′)           Dye   Reverse Primer (5′ to 3′)                             Multiplex 1                                                     Ned       Seq. ID. No. 0013 GGCGGTAAGGACAACGGGGTGTTTGAATTG   Seq. ID. No. 0011               GAACAACCTAAAACCCGCCTCGCCATCG   Seq. ID. No. 0013               GCGCTGGTTTAGCCATCGCCTTCTTCC   Seq. ID. No. 0035                                           Hex       GTGTCAGGTGAGCTACAGCCCGCTTACGCTC   Seq. ID. No. 0037               CAGCCTCCTGCAAACTTTACTGTTCATTTCTACAGTCTC   Seq. ID. No. 0077                                           Fam       GGATCTGTCTGTATCATCATTGAATGAACAACCCATTTC   Seq. ID. No. 0079                                             Hex       GACAAGGTTCTGGCGTGTTACCAACGG   Seq. ID. No. 0103               GTTACAACTCACCTGCGAATTTTTTTAAGTCCC   Seq. ID. No. 0105               Multiplex 2                                    Fam       GATAACATGTCCGGCAAATATTCATTCCCTGAGCA   Seq. ID. No. 0019               GTTTCGCGAATTTTGACAGTTTTTGCATCCTGATC   Seq. ID. No. 0021                                            Hex       GTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGA   Seq. ID. No. 0023               GTCCAGACGCCAGTGCAGCTTATTCTCCACG   Seq. ID. No. 0025                                            Ned       GTTGCCGACCCACAGCGATACGCCAT   Seq. ID. No. 0091               AGCTGATTGCCAGATCGCTTTGCTCCAGAG   Seq. ID. No. 0093                                           Ned       GTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAG   Seq. ID. No. 0099               GCCTGACGCTAAAGATAAAGAAGAAAGCGTCGCG   Seq. ID. No. 0101                                            Hex       GGGTTTGTTTTCAGTGAAGTATTCGCCAAGGTTC   Seq. ID. No. 0115c               GATGTCGAAATGGAAGATTACTCAACATACTGCTTCTC   Seq. ID. No. 0117               Multiplex 3                                           Fam       GCCAGATAAACATCCAGCAGGTCGAACGTCC   Seq. ID. No. 0015               GACTCTGCGGCAATATGGCGTCTTTAGTATCTCCTG   Seq. ID. No. 0017                                           Hex       GGGGCGATCCCACCCTCCATCCTG   Seq. ID. No. 0027               GAGCGGCAATTGTAATCCGGTGGCTTCC   Seq. ID. No. 0029                                           Ned       GGCATCAATAAAAGGTAAGCCAAGTTTCGCCG   Seq. ID. No. 0053               GCATCCTGAACCAACCTGGGTATGCTGC   Seq. ID. No. 0055                                           Fam       GACTGGCGATGAAGAGCGTTTTAATGAGTTTATCAGTGA   Seq. ID. No. 0073               GAATGCGCTGTTCCCCTTCTTCCCTTCC   Seq. ID. No. 0075                                           Hex       GGCGTCCTTCATCGGCCTGTCCGTTAAAC   Seq. ID. No. 0107               GCCGCTGAAAGCCCACACCATGC   Seq. ID. No. 0109                                            Ned       GTTCTTCATACAGCGTCCACGTCGGGCCT   Seq. ID. No. 0127               GACTGGGAGCCATCATTACTTACGCAGCTTGAAC   Seq. ID. No. 0129               Multiplex 4                                   Hex       GACCGGCAATCATCGGGCCAACCA   Seq. ID. No. 0047               GATGCTGGAAAAACTGATGCAGACTCGCGT   Seq. ID. No. 0051               GCAGTTGCTCGGTTTTAACATTGCAGTGATGAC   Seq. ID. No. 0069                                           Hex       GGAAATGGTTTACATGAGTTTGACGATGGCGATC   Seq. ID. No. 0071               GCAGTGATCATTATTAGCACCGCTTTCTGGATGTTC   Seq. ID. No. 0077                                           Hex       GGGGCAGGGAATAAGGCCACCTGTTAAGC   Seq. ID. No. 0079                                           Ned       GCCGGAGGAGGGTGATGAGCGGTTATATTTAGTG   Seq. ID. No. 0083               GCGCTGAAAAGACATTCTCTGTTTGGTTTACACGAC   Seq. ID. No. 0085                                              Fam       GCCGCCCCTTACATTACGCGGACATTC   Seq. ID. No. 0111               GCAGGAGAACAACAAAACAGACAGTAATCAGAGCAGC   Seq. ID. No. 0113               Multiplex 5                                  Ned       GGGCCAGCCGCTGTACCGGGGA   Seq. ID. No. 0007               GTATGATGAAACGCTGACGGCGCTGGATG   Seq. ID. No. 0009                                            Ned       GTCGCTGATAATATTCTCTTTTCGTCATCCCACTGTTAC   Seq. ID. No. 0065               AATACGGTATTGCCATCGGCTCCAAAAAGTTTATC   Seq. ID. No. 0067                                            Hex       GCTCTCCATGGTATCTTCTGACCCAGGGGTATCTA   Seq. ID. No. 0087               GAAAGTTTCATCGGGGGCTGGCTACGGTCTTA   Seq. ID. No. 0089                                            Ned       GTTTCGGGTGAATAGAGGGCGCTTTTCTCGTTA   Seq. ID. No. 0119               GTTCCTCACCAATATTGAAAACACGGCGTAGCAAAAAG   Seq. ID. No. 0121                                             Fam       GCCTGCGGCTGGGCAAATTCGTTCC   Seq. ID. No. 0123               GATGCTCGCCTGATCGACAACAAAATGGTCG   Seq. ID. No. 0125                                           Fam       AACACTTTGTTCCACAAGAAAATTGTCAGGG   Seq. ID. No. 0139               ATTATGTGCATAAAATTGGCATTGCTCTTTT   Seq. ID. No. 0141               Multiplex 6                                           Fam       GAGGGATTGTTACCTTGGTCTCAAAACAATGAAAGG   Seq. ID. No. 0001               GTTCCAGCCCCTTCAACCTTAGCTTATTCTGGCTC   Seq. ID. No. 0003                                            Fam       GCAGCAAACGCCACAGTACCCATGCC   Seq. ID. No. 0057               GTAGGTCATCTGCCGTGGTTCGAGCGCT   Seq. ID. No. 0061                                            Hex       GAAAATCCGGCGACGGTTGCCAGACTC   Seq. ID. No. 0159               GCGGGAGCGGGAAAGACTGCGGA   Seq. ID. No. 0161                 Eight loci polymorphic outside O157:H7 (not multiplexed)                                                           Ned           GCTGTTCCCGTTCTTTGGCTTTACCGCC   Seq. ID. No. 0031               GCGTTACGCCGCAGAACCCACCTGC   Seq. ID. No. 0033                                          Ned       GCCGAAAAACGATGCAGCTGACTTAGGCG   Seq. ID. No. 0095               GACATTTCTGCCCGGGGGTTTGTTTATTTCTGC   Seq. ID. No. 0097                                             Fam       GCCCGCCGGGCCGATGACC   Seq. ID. No. 0131               GGCGGCGTGGGGGATTATTGCCC   Seq. ID. No. 0133                                           Hex       GGGACTGGATATTGTGCAGGGTTCAGCAGG   Seq. ID. No. 0135               GGGCCGGGCAGCGCAAGGTCC   Seq. ID. No. 0137                                             Fam       GCGGCGCATTAGCGTCGTATCAGGC   Seq. ID. No. 0143               CAGTTTGGCCATGCGTCTGGGGTGAC   Seq. ID. No. 0145                                           Fam       GACTGAGGCTGTCATCTCGAAAGAGGGCATTCT   Seq. ID. No. 0147               GCGCTGGGAGGTGTCGCTCAGATGG   Seq. ID. No. 0149                                            Hex       GTTTGCTGTAGCCCAGGCCGTTGATCTTCTTC   Seq. ID. No. 0151               GTTCCGGCGGCGAAAGTTTCCTCGTTAG   Seq. ID. No. 0153                                           Ned       GACTTACTCAGCGCCGCCAACGAAGTCC   Seq. ID. No. 0155               GCACCGCACGTTTCTGAAAAAGCGTCTACT   Seq. ID. No. 0157                            
     [0114]               TABLE 3                          VNTR Locus Attributes.                                 Features of repeat           Array 2  in EDL-933   Location of 5’                                 ORF Identity   Location               (in Sakai   In       Marker 1     if different)   EDL-933   end of array                                         Multiplex 1                       O157-3   6 × 9   OI #7   271423   hypothetical                       protein       O157-9   6 × 11 (6 × 10)   OI #108   3557714   not in an ORF       O157-10   6 × 17.7   OI #108   3559120   hypothetical           (6 × 25.7)           protein       O157-34   18 × 10 (18 × 9)   —   5361545   yjgL       Multiplex 2       O157-5    56 × 2.2   OI #67   2103941   putative                       BigA-like                       protein       O157-6   8 × 4   OI #64   2036603   H repeat-                       associated                       protein of                       Rhs element       O157-30   9 × 3   OI #167   5197093   putative                       histidine                       kinase       O157-33   16 × 3    —   5325245   not in an ORF       O157-39   3 × 4   pOSAK1   1603   hypothetical                       protein       Multiplex 3       O157-4    33 × 2.3   —   1770140   not in an ORF       O157-7    62 × 2.2   —   2716203   fliI       O157-12   29 × 2    OI #134   4360214   putative ATP-                       dependent                       DNA helicase       O157-18    25 × 2.4   —   5462817   hypothetical                       protein       O157-36    7 × 10   pO157   54348   not in an ORF       O157-49   28 × 2    OI #7   258805   hypothetical                       protein       Multiplex 4       O157-11     6 × 5.5   —   4850327   hemX       O157-17   6 × 6 (6 × 8)   OI #174   5456065   hypothetical                       protein       O157-19   6 × 6 (6 × 4)   —   2932247   hypothetical                       protein       O157-25   6 × 5 (6 × 4)   —   1605820   not in an ORF       O157-37   6 × 7   pO157   46468   hypothetical                       protein       Multiplex 5       O157-2   12 × 2    OI #7   252309   putative                       protease       O157-16   21 × 2    OI #172   5385681   hypothetical                       protein       O157-29   6 × 3.5, 3 × 4   on LEE,       4669380 tir               OI #148       O157-45   5 × 4   —   46552   not in an ORF       O157-47   7 × 2   OI #4   152500   not in an ORF       O157-56   5 × 3   OI #55   1785903   hypothetical                       protein       Multiplex 6       O157-1   15 × 4    —   64022   hypothetical                       protein       O157-13   9 × 4   —   4499709   yhjN       O157-68   6 × 3   OI #79   2781280   unknown                       prophage                       CP-933U                       protein                 Eight loci polymorphic outside O157:H7 (not multiplexed)                                 O157-8   19 × 2    —   3367638   not in an ORF       O157-31   9 × 2, 8 × 2   —   5257006   mopA       O157-50   9 × 2   OI #7   248634   putative                       macrophage                       toxin       O157-5   19 × 2    OI #7   267217   Rhs protein       O157-57   6 × 3   —   1304626   appA       O157-58     8 × 2.3   —   1322683   torA       O157-63   6 × 3   —   474840   sbcC       O157-64   6 × 3   —   4253747   50S ribosomal                       subunit                       protein L23                                    
     REFERENCES  
     [0115] 1. Belongia, e. A., M. T. Osterholm, J. T. Solder, D. A. Ammend, J. E. Braun, and K. L. MacDonald. 1993. Transmission of  Escherichia coli  O157:H7 infection in Minnesota child daycare facilities. JAMA. 269:883-888.  
     [0116] 2. Bokete, T., T. Whittham, R. Wilson, C. Clausen, C. O&#39;Callahan, S. Moseley, T. Fritsche, and P. Tarr. 1997. Genetic and phenotypic analysis of  Escherichia coli  with enteropathogenic characteristics isolated from Seattle children. 175:1382-1389.  
     [0117] 3. Centers for Disease Control and Prevention. 2002. Multistate outbreak of  Escherichia coli  O157:H7 infections associated with eating ground beef—United States June-July 2002. MMWR. 51:637-639.  
     [0118] 4. Centers for Disease Control and Prevention. 1997.  Escherichia coli  O157:H7 infections associated with eating a nationally commercial brand of frozen ground beef patties and burgers—Colorado, 1997. MMWR. 46:777-778.  
     [0119] 5. Centers for Disease Control and Prevention. 1996. Lake-associated outbreak of  Escherichia coli  O157:H7—Illinois 1995. MMWR. 45:437-439.  
     [0120] 6. Clark, C., J. Beltrame, and P. Manning. 1991. The oac gene encoding a lipopolysaccharide O-antigen acetylase maps adjacent to the integrase-encoding gene on the genome of  Shigella flexneri  bacteriophage Sf6. Gene. 107:43-52.  
     [0121] 7. Farlow, J., K. L. Smith, J. Wong, M. Abrams, M. Lytle, and P. Keim. 2001.  Francisella tularensis  strain typing using multiple-locus, variable-number tandem repeat analysis. J. Clin. Microbiol. 39:3186-3192.  
     [0122] 8. Feng, P. 1995.  Escherichia coli  serotype O157:H7: novel vehicles of infection and emergence of phenotypic variants. Emerging Infect Dis. 1:47-52.  
     [0123] 9. Grimm, L. M., M. Goldoft, J. Kobayashi, J. H. Lewis, D. Alfi, A. M. Perdichizzi, P. I. Tarr, J. E. Ongerth, S. L. Moseley, and M. Samadpour. 1995. Molecular epidemiology of a fast-food restaurant-associated outbreak of  Escherichia coli  O157:H7 in Washington State. J Clin Microbiol. 33:2155-2158.  
     [0124] 10. Gur-Arie, R., C. J. Cohen, V. Eitan, L. Shelef, E. M. Hallerman, and V. Kashi. 2000. Simple sequence repeats in  Escherichia coli:  abundance, distribution, composition, and polymorphism. Genome Res. 10:62-71.  
     [0125] 11. Ito, T., K Hiramasu, V. Ohshita, and T. Yokota. 1993. Mutations in the rftT gene are responsible for the Ogawa to Inaba serotype conversion in Vibrio cholerae O1. Microbiol Immunol. 37:281-288.  
     [0126] 12. Keene, W., J. McAnulty, F. Hoesly, L. J. Williams, K. Hedberg, G. Oxman, T. Barrett, M. Pfaller, and D. Fleming. 1994. A swimming-associated outbreak of hemorrhagic colitis caused by  Escherichia coli  O157:H7 and  Shigella sonnei.  N Engl J Med 331:579-584.  
     [0127] 13. Keenleyside, W., and C. Whitfield. 1995. Lateral transfer of rfb genes: a mobilizable ColE1-type plasmid carries the rfb O:54  (O:54 antigen biosynthesis) gene cluster from  Salmonella enterica  serovar borreze. J Bacteriol. 177:5247-5253.  
     [0128] 14. Keim, P., L. B. Price, A. M. Klevytska, K. L. Smith, J. M. Schupp, R. Okinaka, P. J. Jackson, and M. E. Hugh-Jones. 2000. Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within  Bacillus anthracis.  J. Bacteriol. 182:2928-2936.  
     [0129] 15. Klevytska, A. M., L. B. Price, J. M. Schupp, P. L. Worsham, J. Wong, and P. Keim. 2001. Identification and characterization of variable-number tandem repeats in the  Yersinia pestis  genome. J. Clin. Microbiol. 39: 3179-3185.  
     [0130] 16. Metzgar, D. E. Thomas, C. Davis, D. Field, and C. Wills. 2001. The microsatellites of  Escherichia coli:  rapidly evolving repetitive DNAs in a non-pathogenic eukaryote. Mol Microbiol. 39:183-190.  
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     [0132] 18. Riley, L. W., R. S. Remis, S. D. Helgerson, H. B. McGee, J. G. Wells, B. R. Davis, R. J. Herbert, E. S. Olcott, L. M. Johnson, N. T. Hargrett, P. A. Blake, and M. L. Cohen. 1983. Hemorrhagic colitis associated with a rare  Escherichia coli  serotype. N Engl J Med 308:681-685.  
     [0133] 19. Swaminathan, B., T. J. Barrett, S. B. Hunter, R. V. Tauxe, and the CDC Pulse Net Task Force. 2001. PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States. Emerging Infect Dis. 7:382-389.  
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     [0137] 23. Whittam, T. S. 1998. Evolution of  Escherichia coli  O157:H7 and other shiga toxin-producing  E. coli  strains, p. 195-209. In J. B. Kaper and A. D. O&#39;Brien (ed.),  Escherichia coli  O157:H7 and other shiga toxin-producing  E. coli  strains, 1 st  ed. American Society for Microbiology, Washington, D.C.  
     [0138] 24. Whittam, T. S. 1996. Genetic variation and evolutionary processes in natural populations of  Escherichia coli,  p. 2708-2720. In F. C. Neidhardt (ed.),  Escherichia coli  and Salmonella: cellular and molecular biology, 2 nd  ed. American Society for Micriobiology, Washington, D.C.  
     [0139] While certain of the preferred embodiments of the present invention have been described and specifically exemplified above, it is not intended that the invention be limited to such embodiments. Various modifications may be made thereto without departing from the scope and spirit of the present invention, as set forth in the following claims.  
    
     
       
         1 
         
           
             560  
           
           
             1  
             36  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            1 

gagggattgt taccttggtc tcaaaacaat gaaagg                               36 

 
           
             2  
             36  
             DNA  
             Escherichia coli O157H7  
           
            2 

cctttcattg ttttgagacc aaggtaacaa tccctc                               36 

 
           
             3  
             35  
             DNA  
             Escherichia coli O157H7  
           
            3 

gttccagccc cttcaacctt agcttattct ggctc                                35 

 
           
             4  
             35  
             DNA  
             Escherichia coli O157H7  
           
            4 

gagccagaat aagctaaggt tgaaggggct ggaac                                35 

 
           
             5  
             36  
             DNA  
             Escherichia coli K-12  
           
            5 

gagggattgt taccttggta aaaaaacaat gaaagg                               36 

 
           
             6  
             36  
             DNA  
             Escherichia coli K-12  
           
            6 

cctttcattg tttttttacc aaggtaacaa tccctc                               36 

 
           
             7  
             22  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            7 

gggccagccg ctgtaccggg ga                                              22 

 
           
             8  
             22  
             DNA  
             Escherichia coli O157H7  
           
            8 

tccccggtac agcggctggc cc                                              22 

 
           
             9  
             29  
             DNA  
             Escherichia coli O157H7  
           
            9 

gtatgatgaa acgctgacgg cgctggatg                                       29 

 
           
             10  
             29  
             DNA  
             Escherichia coli O157H7  
           
            10 

catccagcgc cgtcagcgtt tcatcatac                                       29 

 
           
             11  
             30  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            11 

ggcggtaagg acaacggggt gtttgaattg                                      30 

 
           
             12  
             30  
             DNA  
             Escherichia coli O157H7  
           
            12 

caattcaaac accccgttgt ccttaccgcc                                      30 

 
           
             13  
             28  
             DNA  
             Escherichia coli O157H7  
           
            13 

gaacaaccta aaacccgcct cgccatcg                                        28 

 
           
             14  
             28  
             DNA  
             Escherichia coli O157H7  
           
            14 

cgatggcgag gcgggtttta ggttgttc                                        28 

 
           
             15  
             31  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            15 

gccagataaa catccagcag gtcgaacgtc c                                    31 

 
           
             16  
             31  
             DNA  
             Escherichia coli O157H7  
           
            16 

ggacgttcga cctgctggat gtttatctgg c                                    31 

 
           
             17  
             36  
             DNA  
             Escherichia coli O157H7  
           
            17 

gactctgcgg caatatggcg tctttagtat ctcctg                               36 

 
           
             18  
             36  
             DNA  
             Escherichia coli O157H7  
           
            18 

caggagatac taaagacgcc atattgccgc agagtc                               36 

 
           
             19  
             35  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            19 

gataacatgt ccggcaaata ttcattccct gagca                                35 

 
           
             20  
             35  
             DNA  
             Escherichia coli O157H7  
           
            20 

tgctcaggga atgaatattt gccggacatg ttatc                                35 

 
           
             21  
             35  
             DNA  
             Escherichia coli O157H7  
           
            21 

gtttcgcgaa ttttgacagt ttttgcatcc tgatc                                35 

 
           
             22  
             35  
             DNA  
             Escherichia coli O157H7  
           
            22 

gatcaggatg caaaaactgt caaaattcgc gaaac                                35 

 
           
             23  
             40  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            23 

gtcttcatat tgtttgcgat gtccctgatg aacttattga                           40 

 
           
             24  
             40  
             DNA  
             Escherichia coli O157H7  
           
            24 

tcaataagtt catcagggac atcgcaaaca atatgaagac                           40 

 
           
             25  
             31  
             DNA  
             Escherichia coli O157H7  
           
            25 

gtccagacgc cagtgcagct tattctccac g                                    31 

 
           
             26  
             31  
             DNA  
             Escherichia coli O157H7  
           
            26 

cgtggagaat aagctgcact ggcgtctgga c                                    31 

 
           
             27  
             24  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            27 

ggggcgatcc caccctccat cctg                                            24 

 
           
             28  
             24  
             DNA  
             Escherichia coli O157H7  
           
            28 

caggatggag ggtgggatcg cccc                                            24 

 
           
             29  
             28  
             DNA  
             Escherichia coli O157H7  
           
            29 

gagcggcaat tgtaatccgg tggcttcc                                        28 

 
           
             30  
             28  
             DNA  
             Escherichia coli O157H7  
           
            30 

ggaagccacc ggattacaat tgccgctc                                        28 

 
           
             31  
             28  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            31 

gctgttcccg ttctttggct ttaccgcc                                        28 

 
           
             32  
             28  
             DNA  
             Escherichia coli O157H7  
           
            32 

ggcggtaaag ccaaagaacg ggaacagc                                        28 

 
           
             33  
             25  
             DNA  
             Escherichia coli O157H7  
           
            33 

gcgttacgcc gcagaaccca cctgc                                           25 

 
           
             34  
             25  
             DNA  
             Escherichia coli O157H7  
           
            34 

gcaggtgggt tctgcggcgt aacgc                                           25 

 
           
             35  
             27  
             DNA  
             Escherichia coli O157H7  
           
            35 

gcgctggttt agccatcgcc ttcttcc                                         27 

 
           
             36  
             27  
             DNA  
             Escherichia coli O157H7  
           
            36 

ggaagaaggc gatggctaaa ccagcgc                                         27 

 
           
             37  
             31  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            37 

gtgtcaggtg agctacagcc cgcttacgct c                                    31 

 
           
             38  
             31  
             DNA  
             Escherichia coli O157H7  
           
            38 

gagcgtaagc gggctgtagc tcacctgaca c                                    31 

 
           
             39  
             39  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            39 

cagcctcctg caaactttac tgttcatttc tacagtctc                            39 

 
           
             40  
             39  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            40 

gagactgtag aaatgaacag taaagtttgc aggaggctg                            39 

 
           
             41  
             39  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            41 

cagcctcccc caaactttac tgttcatttc tacagtctc                            39 

 
           
             42  
             39  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            42 

gagactgtag aaatgaacag taaagtttgg gggaggctg                            39 

 
           
             43  
             39  
             DNA  
             Escherichia coli O157H7 Sakai  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            43 

ggatctgtct gtatcatcat tgaatgaaca acccatttc                            39 

 
           
             44  
             39  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            44 

gaaatgggtt gttcattcaa tgatgataca gacagatcc                            39 

 
           
             45  
             40  
             DNA  
             Escherichia coli O157H7 EDL933  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            45 

ggatctgtct gtatcatcca ttgaatgaac aacccatttc                           40 

 
           
             46  
             40  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            46 

gaaatgggtt gttcattcaa tggatgatac agacagatcc                           40 

 
           
             47  
             24  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            47 

gaccggcaat catcgggcca acca                                            24 

 
           
             48  
             24  
             DNA  
             Escherichia coli O157H7  
           
            48 

tggttggccc gatgattgcc ggtc                                            24 

 
           
             49  
             24  
             DNA  
             Escherichia coli K-12  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            49 

ggccggcaat catcgggcca acca                                            24 

 
           
             50  
             24  
             DNA  
             Escherichia coli K-12  
           
            50 

tggttggccc gatgattgcc ggcc                                            24 

 
           
             51  
             30  
             DNA  
             Escherichia coli O157H7  
           
            51 

gatgctggaa aaactgatgc agactcgcgt                                      30 

 
           
             52  
             30  
             DNA  
             Escherichia coli O157H7  
           
            52 

acgcgagtct gcatcagttt ttccagcatc                                      30 

 
           
             53  
             32  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            53 

ggcatcaata aaaggtaagc caagtttcgc cg                                   32 

 
           
             54  
             32  
             DNA  
             Escherichia coli O157H7  
           
            54 

cggcgaaact tggcttacct tttattgatg cc                                   32 

 
           
             55  
             28  
             DNA  
             Escherichia coli O157H7  
           
            55 

gcatcctgaa ccaacctggg tatgctgc                                        28 

 
           
             56  
             28  
             DNA  
             Escherichia coli O157H7  
           
            56 

gcagcatacc caggttggtt caggatgc                                        28 

 
           
             57  
             26  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            57 

gcagcaaacg ccacagtacc catgcc                                          26 

 
           
             58  
             26  
             DNA  
             Escherichia coli O157H7  
           
            58 

ggcatgggta ctgtggcgtt tgctgc                                          26 

 
           
             59  
             26  
             DNA  
             Escherichia coli K-12  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            59 

gcagcagacg ccacagtacc catgcc                                          26 

 
           
             60  
             26  
             DNA  
             Escherichia coli K-12  
           
            60 

ggcatgggta ctgtggcgtc tgctgc                                          26 

 
           
             61  
             28  
             DNA  
             Escherichia coli O157H7  
           
            61 

gtaggtcatc tgccgtggtt cgagcgct                                        28 

 
           
             62  
             28  
             DNA  
             Escherichia coli O157H7  
           
            62 

agcgctcgaa ccacggcaga tgacctac                                        28 

 
           
             63  
             28  
             DNA  
             Escherichia coli K-12  
           
            63 

gtaggtcatc tgccgtggtt cgagcgcg                                        28 

 
           
             64  
             28  
             DNA  
             Escherichia coli K-12  
           
            64 

cgcgctcgaa ccacggcaga tgacctac                                        28 

 
           
             65  
             39  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            65 

gtcgctgata atattctctt ttcgtcatcc cactgttac                            39 

 
           
             66  
             39  
             DNA  
             Escherichia coli O157H7  
           
            66 

gtaacagtgg gatgacgaaa agagaatatt atcagcgac                            39 

 
           
             67  
             35  
             DNA  
             Escherichia coli O157H7  
           
            67 

aatacggtat tgccatcggc tccaaaaagt ttatc                                35 

 
           
             68  
             35  
             DNA  
             Escherichia coli O157H7  
           
            68 

gataaacttt ttggagccga tggcaatacc gtatt                                35 

 
           
             69  
             33  
             DNA  
             Escherichia coli O157H7  
           
            69 

gcagttgctc ggttttaaca ttgcagtgat gac                                  33 

 
           
             70  
             33  
             DNA  
             Escherichia coli O157H7  
           
            70 

gtcatcactg caatgttaaa accgagcaac tgc                                  33 

 
           
             71  
             34  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            71 

ggaaatggtt tacatgagtt tgacgatggc gatc                                 34 

 
           
             72  
             34  
             DNA  
             Escherichia coli O157H7  
           
            72 

gatcgccatc gtcaaactca tgtaaaccat ttcc                                 34 

 
           
             73  
             39  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            73 

gactggcgat gaagagcgtt ttaatgagtt tatcagtga                            39 

 
           
             74  
             39  
             DNA  
             Escherichia coli O157H7  
           
            74 

tcactgataa actcattaaa acgctcttca tcgccagtc                            39 

 
           
             75  
             28  
             DNA  
             Escherichia coli O157H7  
           
            75 

gaatgcgctg ttccccttct tcccttcc                                        28 

 
           
             76  
             28  
             DNA  
             Escherichia coli O157H7  
           
            76 

ggaagggaag aaggggaaca gcgcattc                                        28 

 
           
             77  
             36  
             DNA  
             Escherichia coli O157H7  
           
            77 

gcagtgatca ttattagcac cgctttctgg atgttc                               36 

 
           
             78  
             36  
             DNA  
             Escherichia coli O157H7  
           
            78 

gaacatccag aaagcggtgc taataatgat cactgc                               36 

 
           
             79  
             29  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            79 

ggggcaggga ataaggccac ctgttaagc                                       29 

 
           
             80  
             29  
             DNA  
             Escherichia coli O157H7  
           
            80 

gcttaacagg tggccttatt ccctgcccc                                       29 

 
           
             81  
             28  
             DNA  
             Escherichia coli K-12  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            81 

gggcagggga taaggccacc ggttaagc                                        28 

 
           
             82  
             28  
             DNA  
             Escherichia coli K-12  
           
            82 

gggcagggga taaggccacc ggttaagc                                        28 

 
           
             83  
             34  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            83 

gccggaggag ggtgatgagc ggttatattt agtg                                 34 

 
           
             84  
             34  
             DNA  
             Escherichia coli O157H7  
           
            84 

cactaaatat aaccgctcat caccctcctc cggc                                 34 

 
           
             85  
             36  
             DNA  
             Escherichia coli O157H7  
           
            85 

gcgctgaaaa gacattctct gtttggttta cacgac                               36 

 
           
             86  
             36  
             DNA  
             Escherichia coli O157H7  
           
            86 

gtcgtgtaaa ccaaacagag aatgtctttt cagcgc                               36 

 
           
             87  
             35  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            87 

gctctccatg gtatcttctg acccaggggt atcta                                35 

 
           
             88  
             35  
             DNA  
             Escherichia coli O157H7  
           
            88 

tagatacccc tgggtcagaa gataccatgg agagc                                35 

 
           
             89  
             32  
             DNA  
             Escherichia coli O157H7  
           
            89 

gaaagtttca tcgggggctg gctacggtct ta                                   32 

 
           
             90  
             32  
             DNA  
             Escherichia coli O157H7  
           
            90 

taagaccgta gccagccccc gatgaaactt tc                                   32 

 
           
             91  
             26  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            91 

gttgccgacc cacagcgata cgccat                                          26 

 
           
             92  
             26  
             DNA  
             Escherichia coli O157H7  
           
            92 

atggcgtatc gctgtgggtc ggcaac                                          26 

 
           
             93  
             30  
             DNA  
             Escherichia coli O157H7  
           
            93 

agctgattgc cagatcgctt tgctccagag                                      30 

 
           
             94  
             30  
             DNA  
             Escherichia coli O157H7  
           
            94 

ctctggagca aagcgatctg gcaatcagct                                      30 

 
           
             95  
             29  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            95 

gccgaaaaac gatgcagctg acttaggcg                                       29 

 
           
             96  
             29  
             DNA  
             Escherichia coli O157H7  
           
            96 

cgcctaagtc agctgcatcg tttttcggc                                       29 

 
           
             97  
             33  
             DNA  
             Escherichia coli O157H7  
           
            97 

gacatttctg cccgggggtt tgtttatttc tgc                                  33 

 
           
             98  
             33  
             DNA  
             Escherichia coli O157H7  
           
            98 

gcagaaataa acaaaccccc gggcagaaat gtc                                  33 

 
           
             99  
             37  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            99 

gtgaaggata agctgcattt gtcagtgatg tccgaag                              37 

 
           
             100  
             37  
             DNA  
             Escherichia coli O157H7  
           
            100 

cttcggacat cactgacaaa tgcagcttat ccttcac                              37 

 
           
             101  
             34  
             DNA  
             Escherichia coli O157H7  
           
            101 

gcctgacgct aaagataaag aagaaagcgt cgcg                                 34 

 
           
             102  
             34  
             DNA  
             Escherichia coli O157H7  
           
            102 

cgcgacgctt tcttctttat ctttagcgtc aggc                                 34 

 
           
             103  
             27  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            103 

gacaaggttc tggcgtgtta ccaacgg                                         27 

 
           
             104  
             27  
             DNA  
             Escherichia coli O157H7  
           
            104 

ccgttggtaa cacgccagaa ccttgtc                                         27 

 
           
             105  
             32  
             DNA  
             Escherichia coli O157H7  
           
            105 

gttacaactc acctgcgaat tttttaagtc cc                                   32 

 
           
             106  
             32  
             DNA  
             Escherichia coli O157H7  
           
            106 

gggacttaaa aaattcgcag gtgagttgta ac                                   32 

 
           
             107  
             29  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            107 

ggcgtccttc atcggcctgt ccgttaaac                                       29 

 
           
             108  
             29  
             DNA  
             Escherichia coli O157H7  
           
            108 

gtttaacgga caggccgatg aaggacgcc                                       29 

 
           
             109  
             23  
             DNA  
             Escherichia coli O157H7  
           
            109 

gccgctgaaa gcccacacca tgc                                             23 

 
           
             110  
             23  
             DNA  
             Escherichia coli O157H7  
           
            110 

gcatggtgtg ggctttcagc ggc                                             23 

 
           
             111  
             27  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            111 

gccgcccctt acattacgcg gacattc                                         27 

 
           
             112  
             27  
             DNA  
             Escherichia coli O157H7  
           
            112 

gaatgtccgc gtaatgtaag gggcggc                                         27 

 
           
             113  
             37  
             DNA  
             Escherichia coli O157H7  
           
            113 

gcaggagaac aacaaaacag acagtaatca gagcagc                              37 

 
           
             114  
             37  
             DNA  
             Escherichia coli O157H7  
           
            114 

gctgctctga ttactgtctg ttttgttgtt ctcctgc                              37 

 
           
             115  
             34  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            115 

gggtttgttt tcagtgaagt attcgccaag gttc                                 34 

 
           
             116  
             34  
             DNA  
             Escherichia coli O157H7  
           
            116 

gaaccttggc gaatacttca ctgaaaacaa accc                                 34 

 
           
             117  
             38  
             DNA  
             Escherichia coli O157H7  
           
            117 

gatgtcgaaa tggaagatta ctcaacatac tgcttctc                             38 

 
           
             118  
             38  
             DNA  
             Escherichia coli O157H7  
           
            118 

gagaagcagt atgttgagta atcttccatt tcgacatc                             38 

 
           
             119  
             33  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            119 

gtttcgggtg aatagagggc gcttttctcg tta                                  33 

 
           
             120  
             33  
             DNA  
             Escherichia coli O157H7  
           
            120 

taacgagaaa agcgccctct attcacccga aac                                  33 

 
           
             121  
             38  
             DNA  
             Escherichia coli O157H7  
           
            121 

gttcctcacc aatattgaaa acacggcgta gcaaaaag                             38 

 
           
             122  
             38  
             DNA  
             Escherichia coli O157H7  
           
            122 

ctttttgcta cgccgtgttt tcaatattgg tgaggaac                             38 

 
           
             123  
             25  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            123 

gcctgcggct gggcaaattc gttcc                                           25 

 
           
             124  
             25  
             DNA  
             Escherichia coli O157H7  
           
            124 

ggaacgaatt tgcccagccg caggc                                           25 

 
           
             125  
             31  
             DNA  
             Escherichia coli O157H7  
           
            125 

gatgctcgcc tgatcgacaa caaaatggtc g                                    31 

 
           
             126  
             31  
             DNA  
             Escherichia coli O157H7  
           
            126 

cgaccatttt gttgtcgatc aggcgagcat c                                    31 

 
           
             127  
             29  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            127 

gttcttcata cagcgtccac gtcgggcct                                       29 

 
           
             128  
             29  
             DNA  
             Escherichia coli O157H7  
           
            128 

aggcccgacg tggacgctgt atgaagaac                                       29 

 
           
             129  
             34  
             DNA  
             Escherichia coli O157H7  
           
            129 

gactgggagc catcattact tacgcagctt gaac                                 34 

 
           
             130  
             34  
             DNA  
             Escherichia coli O157H7  
           
            130 

gttcaagctg cgtaagtaat gatggctccc agtc                                 34 

 
           
             131  
             19  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            131 

gcccgccggg ccgatgacc                                                  19 

 
           
             132  
             19  
             DNA  
             Escherichia coli O157H7  
           
            132 

ggtcatcggc ccggcgggc                                                  19 

 
           
             133  
             23  
             DNA  
             Escherichia coli O157H7  
           
            133 

ggcggcgtgg gggattattg ccc                                             23 

 
           
             134  
             23  
             DNA  
             Escherichia coli O157H7  
           
            134 

gggcaataat cccccacgcc gcc                                             23 

 
           
             135  
             30  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            135 

gggactggat attgtgcagg gttcagcagg                                      30 

 
           
             136  
             30  
             DNA  
             Escherichia coli O157H7  
           
            136 

cctgctgaac cctgcacaat atccagtccc                                      30 

 
           
             137  
             21  
             DNA  
             Escherichia coli O157H7  
           
            137 

gggccgggca gcgcaaggtc c                                               21 

 
           
             138  
             21  
             DNA  
             Escherichia coli O157H7  
           
            138 

ggaccttgcg ctgcccggcc c                                               21 

 
           
             139  
             31  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            139 

aacactttgt tccacaagaa aattgtcagg g                                    31 

 
           
             140  
             31  
             DNA  
             Escherichia coli O157H7  
           
            140 

ccctgacaat tttcttgtgg aacaaagtgt t                                    31 

 
           
             141  
             31  
             DNA  
             Escherichia coli O157H7  
           
            141 

attatgtgca taaaattggc attgctcttt t                                    31 

 
           
             142  
             31  
             DNA  
             Escherichia coli O157H7  
           
            142 

aaaagagcaa tgccaatttt atgcacataa t                                    31 

 
           
             143  
             25  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            143 

gcggcgcatt agcgtcgtat caggc                                           25 

 
           
             144  
             25  
             DNA  
             Escherichia coli O157H7  
           
            144 

gcctgatacg acgctaatgc gccgc                                           25 

 
           
             145  
             26  
             DNA  
             Escherichia coli O157H7  
           
            145 

cagtttggcc atgcgtctgg ggtgac                                          26 

 
           
             146  
             26  
             DNA  
             Escherichia coli O157H7  
           
            146 

gtcaccccag acgcatggcc aaactg                                          26 

 
           
             147  
             33  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               FAM labeled  
             
           
            147 

gactgaggct gtcatctcga aagagggcat tct                                  33 

 
           
             148  
             33  
             DNA  
             Escherichia coli O157H7  
           
            148 

agaatgccct ctttcgagat gacagcctca gtc                                  33 

 
           
             149  
             25  
             DNA  
             Escherichia coli O157H7  
           
            149 

gcgctgggag gtgtcgctca gatgg                                           25 

 
           
             150  
             25  
             DNA  
             Escherichia coli O157H7  
           
            150 

ccatctgagc gacacctccc agcgc                                           25 

 
           
             151  
             32  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            151 

gtttgctgta gcccaggccg ttgatcttct tc                                   32 

 
           
             152  
             32  
             DNA  
             Escherichia coli O157H7  
           
            152 

gaagaagatc aacggcctgg gctacagcaa ac                                   32 

 
           
             153  
             28  
             DNA  
             Escherichia coli O157H7  
           
            153 

gttccggcgg cgaaagtttc ctcgttag                                        28 

 
           
             154  
             28  
             DNA  
             Escherichia coli O157H7  
           
            154 

ctaacgagga aactttcgcc gccggaac                                        28 

 
           
             155  
             28  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               NED labeled  
             
           
            155 

gacttactca gcgccgccaa cgaagtcc                                        28 

 
           
             156  
             28  
             DNA  
             Escherichia coli O157H7  
           
            156 

ggacttcgtt ggcggcgctg agtaagtc                                        28 

 
           
             157  
             30  
             DNA  
             Escherichia coli O157H7  
           
            157 

gcaccgcacg tttctgaaaa agcgtctact                                      30 

 
           
             158  
             30  
             DNA  
             Escherichia coli O157H7  
           
            158 

agtagacgct ttttcagaaa cgtgcggtgc                                      30 

 
           
             159  
             27  
             DNA  
             Escherichia coli O157H7  
             
               misc_feature  
               1  
               HEX labeled  
             
           
            159 

gaaaatccgg cgacggttgc cagactc                                         27 

 
           
             160  
             27  
             DNA  
             Escherichia coli O157H7  
           
            160 

gagtctggca accgtcgccg gattttc                                         27 

 
           
             161  
             23  
             DNA  
             Escherichia coli O157H7  
           
            161 

gcgggagcgg gaaagactgc gga                                             23 

 
           
             162  
             23  
             DNA  
             Escherichia coli O157H7  
           
            162 

tccgcagtct ttcccgctcc cgc                                             23 

 
           
             163  
             292  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            163 

gagggattgt taccttggtc tcaaaacaat gaaaggtcac aataaagtcc tttacttgga     60 

acctgggtgg aatgctaacc tggacggtgc taccctggac ggtgctaccc tggacggtgc    120 

taccgtggac ggtgctaccc acctatatga tgaggtaatt attattaata aaatcacccc    180 

caaaaaaatt gatactgaag aagttgctac taaacaaagt actgctgaac aaattactga    240 

caacgcaatt attgaatgag ccagaataag ctaaggttga aggggctgga ac            292 

 
           
             164  
             292  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            164 

gttccagccc cttcaacctt agcttattct ggctcattca ataattgcgt tgtcagtaat     60 

ttgttcagca gtactttgtt tagtagcaac ttcttcagta tcaatttttt tgggggtgat    120 

tttattaata ataattacct catcatatag gtgggtagca ccgtccacgg tagcaccgtc    180 

cagggtagca ccgtccaggg tagcaccgtc caggttagca ttccacccag gttccaagta    240 

aaggacttta ttgtgacctt tcattgtttt gagaccaagg taacaatccc tc            292 

 
           
             165  
             292  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            165 

gagggattgt taccttggtc tcaaaacaat gaaaggtcac aataaagtcc tttacttgga     60 

acctgggtgg aatgctaacc tggacggtgc taccctggac ggtgctaccc tggacggtgc    120 

taccgtggac ggtgctaccc acctatatga tgaggtaatt attattaata aaatcacccc    180 

caaaaaaatt gatactgaag aagttgctac taaacaaagt actgctgaac aaattactga    240 

caacgcaatt attgaatgag ccagaataag ctaaggttga aggggctgga ac            292 

 
           
             166  
             292  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            166 

gttccagccc cttcaacctt agcttattct ggctcattca ataattgcgt tgtcagtaat     60 

ttgttcagca gtactttgtt tagtagcaac ttcttcagta tcaatttttt tgggggtgat    120 

tttattaata ataattacct catcatatag gtgggtagca ccgtccacgg tagcaccgtc    180 

cagggtagca ccgtccaggg tagcaccgtc caggttagca ttccacccag gttccaagta    240 

aaggacttta ttgtgacctt tcattgtttt gagaccaagg taacaatccc tc            292 

 
           
             167  
             335  
             DNA  
             Escherichia coli K-12  
           
            167 

gagggattgt taccttggta aaaaaacaat gaaaggtagc aatgatatcc tttatgagag     60 

acctgggtgg aatgctaacc tgggcgtgct accccggacg gtgctacccc ggacggtgct    120 

aacccggacg gtgctaacct ggacggtgct accgtgaacg gtgctacctc cttatatgat    180 

gaggtaatta ttattaataa aatccccccc aaaaaaattg atactaaagg agttgctact    240 

gaagaagttg ctactaaaaa agtactgctg aacaaattac tgacaacgca attattgaat    300 

gagccagaat aagctaaggt tgaaggggct ggaac                               335 

 
           
             168  
             335  
             DNA  
             Escherichia coli K-12  
           
            168 

gttccagccc cttcaacctt agcttattct ggctcattca ataattgcgt tgtcagtaat     60 

ttgttcagca gtactttttt agtagcaact tcttcagtag caactccttt agtatcaatt    120 

tttttggggg ggattttatt aataataatt acctcatcat ataaggaggt agcaccgttc    180 

acggtagcac cgtccaggtt agcaccgtcc gggttagcac cgtccggggt agcaccgtcc    240 

ggggtagcac gcccaggtta gcattccacc caggtctctc ataaaggata tcattgctac    300 

ctttcattgt ttttttacca aggtaacaat ccctc                               335 

 
           
             169  
             278  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            169 

gggccagccg ctgtaccggg gaaactgttt cagcggtctc ttccggctgt tctctgtcgg     60 

tctcatcagc cggtacggca ccggtattat cctgttctga ctctgactct gactctggct    120 

gcgtatcctc cacagtatct gcgtccggca acggcgccgc atcgtcctct gccacgccca    180 

gtaactgctg gcgcagcgcg ataatctccc ggaccagcgt ctgctgctgg tgccaagccg    240 

cttccagttc atccagcgcc gtcagcgttt catcatac                            278 

 
           
             170  
             278  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            170 

gtatgatgaa acgctgacgg cgctggatga actggaagcg gcttggcacc agcagcagac     60 

gctggtccgg gagattatcg cgctgcgcca gcagttactg ggcgtggcag aggacgatgc    120 

ggcgccgttg ccggacgcag atactgtgga ggatacgcag ccagagtcag agtcagagtc    180 

agaacaggat aataccggtg ccgtaccggc tgatgagacc gacagagaac agccggaaga    240 

gaccgctgaa acagtttccc cggtacagcg gctggccc                            278 

 
           
             171  
             278  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            171 

gggccagccg ctgtaccggg gaaactgttt cagcggtctc ttccggctgt tctctgtcgg     60 

tctcatcagc cggtacggca ccggtattat cctgttctga ctctgactct gactctggct    120 

gcgtatcctc cacagtatct gcgtccggca acggcgccgc atcgtcctct gccacgccca    180 

gtaactgctg gcgcagcgcg ataatctccc ggaccagcgt ctgctgctgg tgccaagccg    240 

cttccagttc atccagcgcc gtcagcgttt catcatac                            278 

 
           
             172  
             278  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            172 

gtatgatgaa acgctgacgg cgctggatga actggaagcg gcttggcacc agcagcagac     60 

gctggtccgg gagattatcg cgctgcgcca gcagttactg ggcgtggcag aggacgatgc    120 

ggcgccgttg ccggacgcag atactgtgga ggatacgcag ccagagtcag agtcagagtc    180 

agaacaggat aataccggtg ccgtaccggc tgatgagacc gacagagaac agccggaaga    240 

gaccgctgaa acagtttccc cggtacagcg gctggccc                            278 

 
           
             173  
             377  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            173 

ggcggtaagg acaacggggt gtttgaattg gtgccagacg agaaacaacc aggggacttt     60 

cataagttta cacctacagg aagcaaatag taatgaaagt atttactttt ttgttgatta    120 

tcatggtatg tctttataac tttggagtga gggctgctat ggataactca aaacattctg    180 

atgaggcgga aaaattattg gcggagcttt ctgctagaaa aggtgaaggt gaaggtgaag    240 

gtgaaggtga aggtgaaggt gaaggtgaag gtgaaccaaa atcaacagta agcgtctttt    300 

atttgcagcc agaagaagta aatactttat cacaccaggc aaaacgaggc gatggcgagg    360 

cgggttttag gttgttc                                                   377 

 
           
             174  
             377  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            174 

gaacaaccta aaacccgcct cgccatcgcc tcgttttgcc tggtgtgata aagtatttac     60 

ttcttctggc tgcaaataaa agacgcttac tgttgatttt ggttcacctt caccttcacc    120 

ttcaccttca ccttcacctt caccttcacc ttcacctttt ctagcagaaa gctccgccaa    180 

taatttttcc gcctcatcag aatgttttga gttatccata gcagccctca ctccaaagtt    240 

ataaagacat accatgataa tcaacaaaaa agtaaatact ttcattacta tttgcttcct    300 

gtaggtgtaa acttatgaaa gtcccctggt tgtttctcgt ctggcaccaa ttcaaacacc    360 

ccgttgtcct taccgcc                                                   377 

 
           
             175  
             377  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            175 

ggcggtaagg acaacggggt gtttgaattg gtgccagacg agaaacaacc aggggacttt     60 

cataagttta cacctacagg aagcaaatag taatgaaagt atttactttt ttgttgatta    120 

tcatggtatg tctttataac tttggagtga gggctgctat ggataactca aaacattctg    180 

atgaggcgga aaaattattg gcggagcttt ctgctagaaa aggtgaaggt gaaggtgaag    240 

gtgaaggtga aggtgaaggt gaaggtgaag gtgaaccaaa atcaacagta agcgtctttt    300 

atttgcagcc agaagaagta aatactttat cacaccaggc aaaacgaggc gatggcgagg    360 

cgggttttag gttgttc                                                   377 

 
           
             176  
             377  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            176 

gaacaaccta aaacccgcct cgccatcgcc tcgttttgcc tggtgtgata aagtatttac     60 

ttcttctggc tgcaaataaa agacgcttac tgttgatttt ggttcacctt caccttcacc    120 

ttcaccttca ccttcacctt caccttcacc ttcacctttt ctagcagaaa gctccgccaa    180 

taatttttcc gcctcatcag aatgttttga gttatccata gcagccctca ctccaaagtt    240 

ataaagacat accatgataa tcaacaaaaa agtaaatact ttcattacta tttgcttcct    300 

gtaggtgtaa acttatgaaa gtcccctggt tgtttctcgt ctggcaccaa ttcaaacacc    360 

ccgttgtcct taccgcc                                                   377 

 
           
             177  
             331  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            177 

gccagataaa catccagcag gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg     60 

tccttattca tagaatcgat cgtcgccata cgcgcacctc attgttgtcg gcgctctctg    120 

tgtggagcac ctcattgttg tcggcgctct ctgtgtggag cacctcattt caagcataga    180 

acacctgtta aaaaccgcgt cgccggagaa tttttttctt tgcgatttct tattatcaga    240 

gtgccactaa tccgtttctg aacggaattt tatgctggat aaaaagggcg ttcagcagga    300 

gatactaaag acgccatatt gccgcagagt c                                   331 

 
           
             178  
             331  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            178 

gactctgcgg caatatggcg tctttagtat ctcctgctga acgccctttt tatccagcat     60 

aaaattccgt tcagaaacgg attagtggca ctctgataat aagaaatcgc aaagaaaaaa    120 

attctccggc gacgcggttt ttaacaggtg ttctatgctt gaaatgaggt gctccacaca    180 

gagagcgccg acaacaatga ggtgctccac acagagagcg ccgacaacaa tgaggtgcgc    240 

gtatggcgac gatcgattct atgaataagg acaccacacg tttgagcgat ggacccgact    300 

ggacgttcga cctgctggat gtttatctgg c                                   331 

 
           
             179  
             331  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            179 

gccagataaa catccagcag gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg     60 

tccttattca tagaatcgat cgtcgccata cgcgcacctc attgttgtcg gcgctctctg    120 

tgtggagcac ctcattgttg tcggcgctct ctgtgtggag cacctcattt caagcataga    180 

acacctgtta aaaaccgcgt cgccggagaa tttttttctt tgcgatttct tattatcaga    240 

gtgccactaa tccgtttctg aacggaattt tatgctggat aaaaagggcg ttcagcagga    300 

gatactaaag acgccatatt gccgcagagt c                                   331 

 
           
             180  
             331  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            180 

gactctgcgg caatatggcg tctttagtat ctcctgctga acgccctttt tatccagcat     60 

aaaattccgt tcagaaacgg attagtggca ctctgataat aagaaatcgc aaagaaaaaa    120 

attctccggc gacgcggttt ttaacaggtg ttctatgctt gaaatgaggt gctccacaca    180 

gagagcgccg acaacaatga ggtgctccac acagagagcg ccgacaacaa tgaggtgcgc    240 

gtatggcgac gatcgattct atgaataagg acaccacacg tttgagcgat ggacccgact    300 

ggacgttcga cctgctggat gtttatctgg c                                   331 

 
           
             181  
             298  
             DNA  
             Escherichia coli K-12  
           
            181 

gccagataaa catccagcag gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg     60 

tccttattca tagaatcgat cgtcgccata cgcgcacctc attgttgtcg gcgctctctg    120 

tgtggagcac ctcatttcaa gcatagaaca cctgttaaaa accgcgtcgc cggagaattt    180 

ttttctttgc gatttcttat tatcagagtg ccactaatcc gcttctgaac ggaattttat    240 

gctggataaa aagggcgttc agcaggagat actaaagacg ccatattgcc gcagagtc      298 

 
           
             182  
             298  
             DNA  
             Escherichia coli K-12  
           
            182 

gactctgcgg caatatggcg tctttagtat ctcctgctga acgccctttt tatccagcat     60 

aaaattccgt tcagaagcgg attagtggca ctctgataat aagaaatcgc aaagaaaaaa    120 

attctccggc gacgcggttt ttaacaggtg ttctatgctt gaaatgaggt gctccacaca    180 

gagagcgccg acaacaatga ggtgcgcgta tggcgacgat cgattctatg aataaggaca    240 

ccacacgttt gagcgatgga cccgactgga cgttcgacct gctggatgtt tatctggc      298 

 
           
             183  
             468  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            183 

gataacatgt ccggcaaata ttcattccct gagcaaagag cagctagaaa atttatccgc     60 

ggagtgcaga gaaaataaag atagtgcagt tttaccatgg gcagctgcag gtcttgctgc    120 

agtagctacg ggaattgcta tatatacctt aagtgacgat gacaatcacc accataataa    180 

ctccccagtt ccggatgatg gcggtgatac gcctgttccg ccagacgacg gcggtgatac    240 

gccagttccg gatgatggcg gtgatacgcc tgttccgcca gacgacggcg gtgatacgcc    300 

agttccgcct gatgacggcg gtgatacgcc ggttccgcca gatgacggcg gtgatacgcc    360 

agttccgcca gacgatggtg gcgatacccc ggttaaacac aatcctgtcg tctataaaaa    420 

tggcgtaact tgggatcagg atgcaaaaac tgtcaaaatt cgcgaaac                 468 

 
           
             184  
             468  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            184 

gtttcgcgaa ttttgacagt ttttgcatcc tgatcccaag ttacgccatt tttatagacg     60 

acaggattgt gtttaaccgg ggtatcgcca ccatcgtctg gcggaactgg cgtatcaccg    120 

ccgtcatctg gcggaaccgg cgtatcaccg ccgtcatcag gcggaactgg cgtatcaccg    180 

ccgtcgtctg gcggaacagg cgtatcaccg ccatcatccg gaactggcgt atcaccgccg    240 

tcgtctggcg gaacaggcgt atcaccgcca tcatccggaa ctggggagtt attatggtgg    300 

tgattgtcat cgtcacttaa ggtatatata gcaattcccg tagctactgc agcaagacct    360 

gcagctgccc atggtaaaac tgcactatct ttattttctc tgcactccgc ggataaattt    420 

tctagctgct ctttgctcag ggaatgaata tttgccggac atgttatc                 468 

 
           
             185  
             469  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            185 

gataacatgt ccggcaaata ttcattccct gagcaaagag cagctagaaa atttatccgc     60 

ggagtgcaga gaaaataaag atagtgcagt tttaccatgg gcagctgcag gtcttgctgc    120 

agtagctacg ggaattgcta tatatacctt aagtgacgat gacaatcacc accataataa    180 

ctccccagtt ccggatgatg gcggtgatac gcctgttccg ccagacgacg gcggtgatac    240 

gccagttccg gatgatggcg gtgatacgcc tgttccgcca gacgacggcg gtgatacgcc    300 

agttccgcct gatgacgggc ggtgatacgc cggttccgcc agatgacggc ggtgatacgc    360 

cagttccgcc agacgatggt ggcgataccc cggttaaaca caatcctgtc gtctataaaa    420 

atggcgtaac ttgggatcag gatgcaaaaa ctgtcaaaat tcgcgaaac                469 

 
           
             186  
             469  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            186 

gtttcgcgaa ttttgacagt ttttgcatcc tgatcccaag ttacgccatt tttatagacg     60 

acaggattgt gtttaaccgg ggtatcgcca ccatcgtctg gcggaactgg cgtatcaccg    120 

ccgtcatctg gcggaaccgg cgtatcaccg cccgtcatca ggcggaactg gcgtatcacc    180 

gccgtcgtct ggcggaacag gcgtatcacc gccatcatcc ggaactggcg tatcaccgcc    240 

gtcgtctggc ggaacaggcg tatcaccgcc atcatccgga actggggagt tattatggtg    300 

gtgattgtca tcgtcactta aggtatatat agcaattccc gtagctactg cagcaagacc    360 

tgcagctgcc catggtaaaa ctgcactatc tttattttct ctgcactccg cggataaatt    420 

ttctagctgc tctttgctca gggaatgaat atttgccgga catgttatc                469 

 
           
             187  
             260  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            187 

gtcttcatat tgtttgcgat gtccctgatg aacttattga tttcacgttt gaatggaaag     60 

ggctgaagaa attatgcgtg gcagtctcct ttcggtcgat aatagcagaa cagcagaaca    120 

gcagaacagc agaacaaaag aaagagccaa aaaatgacgg tcagatatta tatcagttct    180 

gctgatttaa ccgcagagaa gttcgcacag caatccgaaa ccactggcac gtggagaata    240 

agctgcactg gcgtctggac                                                260 

 
           
             188  
             260  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            188 

gtccagacgc cagtgcagct tattctccac gtgccagtgg tttcggattg ctgtgcgaac     60 

ttctctgcgg ttaaatcagc agaactgata taatatctga ccgtcatttt ttggctcttt    120 

cttttgttct gctgttctgc tgttctgctg ttctgctatt atcgaccgaa aggagactgc    180 

cacgcataat ttcttcagcc ctttccattc aaacgtgaaa tcaataagtt catcagggac    240 

atcgcaaaca atatgaagac                                                260 

 
           
             189  
             260  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            189 

gtcttcatat tgtttgcgat gtccctgatg aacttattga tttcacgttt gaatggaaag     60 

ggctgaagaa attatgcgtg gcagtctcct ttcggtcgat aatagcagaa cagcagaaca    120 

gcagaacagc agaacaaaag aaagagccaa aaaatgacgg tcagatatta tatcagttct    180 

gctgatttaa ccgcagagaa gttcgcacag caatccgaaa ccactggcac gtggagaata    240 

agctgcactg gcgtctggac                                                260 

 
           
             190  
             260  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            190 

gtccagacgc cagtgcagct tattctccac gtgccagtgg tttcggattg ctgtgcgaac     60 

ttctctgcgg ttaaatcagc agaactgata taatatctga ccgtcatttt ttggctcttt    120 

cttttgttct gctgttctgc tgttctgctg ttctgctatt atcgaccgaa aggagactgc    180 

cacgcataat ttcttcagcc ctttccattc aaacgtgaaa tcaataagtt catcagggac    240 

atcgcaaaca atatgaagac                                                260 

 
           
             191  
             236  
             DNA  
             Escherichia coli K-12  
           
            191 

gtcttcatat tgtttgcgat gtccctgatg aacttattga tttcacgttt gaatggaaag     60 

gactgaagaa attatgcgtg gcagtctcct ttcggtcaat aatagcagaa caaaagaaag    120 

agccagaaat gacggtcaga tattatatca gttctgctga tttaaccgca gaaaagttcg    180 

ccacagcaat ccgaaaccac tggcacgtgg agaataagct gcactggcgt ctggac        236 

 
           
             192  
             236  
             DNA  
             Escherichia coli K-12  
           
            192 

gtccagacgc cagtgcagct tattctccac gtgccagtgg tttcggattg ctgtggcgaa     60 

cttttctgcg gttaaatcag cagaactgat ataatatctg accgtcattt ctggctcttt    120 

cttttgttct gctattattg accgaaagga gactgccacg cataatttct tcagtccttt    180 

ccattcaaac gtgaaatcaa taagttcatc agggacatcg caaacaatat gaagac        236 

 
           
             193  
             327  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            193 

ggggcgatcc caccctccat cctggcggct gtaaagtctc cgccgatgaa ggcgatctcg     60 

acgccagtgt cgccactcgc tggcaagaac tctgccgtct ggcagcacca ggagtggtgt    120 

aatgaccacg cgcctgactc gctggcaaga actctgccgt ctggcagcac caggagtggt    180 

gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga agccaaaatg    240 

gcgcagttgc ctgcggtacg tcgctacggg cgattaaccc gcgctaccgg gctggtgctg    300 

gaagccaccg gattacaatt gccgctc                                        327 

 
           
             194  
             327  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            194 

gagcggcaat tgtaatccgg tggcttccag caccagcccg gtagcgcggg ttaatcgccc     60 

gtagcgacgt accgcaggca actgcgccat tttggcttca aagttatcca gcgtggttag    120 

ccagcgagtc aggcgcgtgg tcattacacc actcctggtg ctgccagacg gcagagttct    180 

tgccagcgag tcaggcgcgt ggtcattaca ccactcctgg tgctgccaga cggcagagtt    240 

cttgccagcg agtggcgaca ctggcgtcga gatcgccttc atcggcggag actttacagc    300 

cgccaggatg gagggtggga tcgcccc                                        327 

 
           
             195  
             327  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            195 

ggggcgatcc caccctccat cctggcggct gtaaagtctc cgccgatgaa ggcgatctcg     60 

acgccagtgt cgccactcgc tggcaagaac tctgccgtct ggcagcacca ggagtggtgt    120 

aatgaccacg cgcctgactc gctggcaaga actctgccgt ctggcagcac caggagtggt    180 

gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga agccaaaatg    240 

gcgcagttgc ctgcggtacg tcgctacggg cgattaaccc gcgctaccgg gctggtgctg    300 

gaagccaccg gattacaatt gccgctc                                        327 

 
           
             196  
             327  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            196 

gagcggcaat tgtaatccgg tggcttccag caccagcccg gtagcgcggg ttaatcgccc     60 

gtagcgacgt accgcaggca actgcgccat tttggcttca aagttatcca gcgtggttag    120 

ccagcgagtc aggcgcgtgg tcattacacc actcctggtg ctgccagacg gcagagttct    180 

tgccagcgag tcaggcgcgt ggtcattaca ccactcctgg tgctgccaga cggcagagtt    240 

cttgccagcg agtggcgaca ctggcgtcga gatcgccttc atcggcggag actttacagc    300 

cgccaggatg gagggtggga tcgcccc                                        327 

 
           
             197  
             265  
             DNA  
             Escherichia coli K-12  
           
            197 

ggggcgatcc caccctccat cctggcggct gtaaagtctc cgccgatgaa ggcgatctcg     60 

acgccagtgt cgccactcgc tggcaagaac tctgccgtct ggcagcacca ggagtggtgt    120 

aatgaccacg cgcctgactc gctggctaac cacgctggat aactttgaag ccaaaatggc    180 

gcagttgcct gcggtacgtc gctacgggcg attaacccgc gctaccgggc tggtgctgga    240 

agccaccgga ttacaattgc cgctc                                          265 

 
           
             198  
             265  
             DNA  
             Escherichia coli K-12  
           
            198 

gagcggcaat tgtaatccgg tggcttccag caccagcccg gtagcgcggg ttaatcgccc     60 

gtagcgacgt accgcaggca actgcgccat tttggcttca aagttatcca gcgtggttag    120 

ccagcgagtc aggcgcgtgg tcattacacc actcctggtg ctgccagacg gcagagttct    180 

tgccagcgag tggcgacact ggcgtcgaga tcgccttcat cggcggagac tttacagccg    240 

ccaggatgga gggtgggatc gcccc                                          265 

 
           
             199  
             249  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            199 

gctgttcccg ttctttggct ttaccgccga gaatattgtg gcaaaagcgc ataaggtgct     60 

gggagtaaaa ggtgcctgat ggtgatcgcc ggatgctgat tgccggatgc gacgctgacg    120 

cgtcttatcc ggcctacagc gtcttatccg gcctacatgt cccgccattt tgtttaacgg    180 

gtgatccaca acgtgggcca ggcgtctggc ccatgccagt tatcgcaggt gggttctgcg    240 

gcgtaacgc                                                            249 

 
           
             200  
             249  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            200 

gcgttacgcc gcagaaccca cctgcgataa ctggcatggg ccagacgcct ggcccacgtt     60 

gtggatcacc cgttaaacaa aatggcggga catgtaggcc ggataagacg ctgtaggccg    120 

gataagacgc gtcagcgtcg catccggcaa tcagcatccg gcgatcacca tcaggcacct    180 

tttactccca gcaccttatg cgcttttgcc acaatattct cggcggtaaa gccaaagaac    240 

gggaacagc                                                            249 

 
           
             201  
             249  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            201 

gctgttcccg ttctttggct ttaccgccga gaatattgtg gcaaaagcgc ataaggtgct     60 

gggagtaaaa ggtgcctgat ggtgatcgcc ggatgctgat tgccggatgc gacgctgacg    120 

cgtcttatcc ggcctacagc gtcttatccg gcctacatgt cccgccattt tgtttaacgg    180 

gtgatccaca acgtgggcca ggcgtctggc ccatgccagt tatcgcaggt gggttctgcg    240 

gcgtaacgc                                                            249 

 
           
             202  
             249  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            202 

gcgttacgcc gcagaaccca cctgcgataa ctggcatggg ccagacgcct ggcccacgtt     60 

gtggatcacc cgttaaacaa aatggcggga catgtaggcc ggataagacg ctgtaggccg    120 

gataagacgc gtcagcgtcg catccggcaa tcagcatccg gcgatcacca tcaggcacct    180 

tttactccca gcaccttatg cgcttttgcc acaatattct cggcggtaaa gccaaagaac    240 

gggaacagc                                                            249 

 
           
             203  
             249  
             DNA  
             Escherichia coli K-12  
           
            203 

gctgttcccg ttctttggct ttaccgccga gaatattgtg gcaaaagcgc ataaggtgct     60 

gggagtgaaa ggtgcctgat ggtgattgcc ggatgctgat tgccggatgc gacgctgacg    120 

cgtcttatcc ggcctacagc gtcttatccg gcctacatgt cccgccattt tgtttaacgg    180 

gtgatccaca acgtgggcca ggcgtctggc ccatgccagt tatcgcaggt gggttctgcg    240 

gcgtaacgc                                                            249 

 
           
             204  
             249  
             DNA  
             Escherichia coli K-12  
           
            204 

gcgttacgcc gcagaaccca cctgcgataa ctggcatggg ccagacgcct ggcccacgtt     60 

gtggatcacc cgttaaacaa aatggcggga catgtaggcc ggataagacg ctgtaggccg    120 

gataagacgc gtcagcgtcg catccggcaa tcagcatccg gcaatcacca tcaggcacct    180 

ttcactccca gcaccttatg cgcttttgcc acaatattct cggcggtaaa gccaaagaac    240 

gggaacagc                                                            249 

 
           
             205  
             526  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            205 

gcgctggttt agccatcgcc ttcttcctcc gtaatggttt tctgaatttg gccacctgaa     60 

cagagctcac caaagctatg gatgtcggta tttccacaat accaagatgg cgaaaataac    120 

tgcatgataa gcctcaggga aaagggaaga cactaccccc gataattcag aaacaaatcg    180 

aaatacatga actaaagaaa aaatcacaac aaatagaaat agaaatagaa atagaaatag    240 

aaatagaaat agaaatagaa atagaaatat taaaacagac cactgtagat tcaattggtc    300 

aacgcaacag ttatgtgaaa acatggggtt gcggagtttt tttgaatgag acgaacattt    360 

acagcagagg aaaaagcctc tgtttttgaa ctatggaaga acggaacagg cttcagtgaa    420 

atagcgaata tcctgggttc aaaacccgga acgatcttca ctatgttaag ggatactggc    480 

ggcataaaac cccatgagcg taagcgggct gtagctcacc tgacac                   526 

 
           
             206  
             526  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            206 

gtgtcaggtg agctacagcc cgcttacgct catggggttt tatgccgcca gtatccctta     60 

acatagtgaa gatcgttccg ggttttgaac ccaggatatt cgctatttca ctgaagcctg    120 

ttccgttctt ccatagttca aaaacagagg ctttttcctc tgctgtaaat gttcgtctca    180 

ttcaaaaaaa ctccgcaacc ccatgttttc acataactgt tgcgttgacc aattgaatct    240 

acagtggtct gttttaatat ttctatttct atttctattt ctatttctat ttctatttct    300 

atttctattt ctatttgttg tgattttttc tttagttcat gtatttcgat ttgtttctga    360 

attatcgggg gtagtgtctt cccttttccc tgaggcttat catgcagtta ttttcgccat    420 

cttggtattg tggaaatacc gacatccata gctttggtga gctctgttca ggtggccaaa    480 

ttcagaaaac cattacggag gaagaaggcg atggctaaac cagcgc                   526 

 
           
             207  
             532  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            207 

gcgctggttt agccatcgcc ttcttcctcc gtaatggttt tctgaatttg gccacctgaa     60 

cagagctcac caaagctatg gatgtcggta tttccacaat accaagatgg cgaaaataac    120 

tgcatgataa gcctcaggga aaagggaaga cactaccccc gataattcag aaacaaatcg    180 

aaatacatga actaaagaaa aaatcacaac aaatagaaat agaaatagaa atagaaatag    240 

aaatagaaat agaaatagaa atagaaatag aaatattaaa acagaccact gtagattcaa    300 

ttggtcaacg caacagttat gtgaaaacat ggggttgcgg agtttttttg aatgagacga    360 

acatttacag cagaggaaaa agcctctgtt tttgaactat ggaagaacgg aacaggcttc    420 

agtgaaatag cgaatatcct gggttcaaaa cccggaacga tcttcactat gttaagggat    480 

actggcggca taaaacccca tgagcgtaag cgggctgtag ctcacctgac ac            532 

 
           
             208  
             532  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            208 

gtgtcaggtg agctacagcc cgcttacgct catggggttt tatgccgcca gtatccctta     60 

acatagtgaa gatcgttccg ggttttgaac ccaggatatt cgctatttca ctgaagcctg    120 

ttccgttctt ccatagttca aaaacagagg ctttttcctc tgctgtaaat gttcgtctca    180 

ttcaaaaaaa ctccgcaacc ccatgttttc acataactgt tgcgttgacc aattgaatct    240 

acagtggtct gttttaatat ttctatttct atttctattt ctatttctat ttctatttct    300 

atttctattt ctatttctat ttgttgtgat tttttcttta gttcatgtat ttcgatttgt    360 

ttctgaatta tcgggggtag tgtcttccct tttccctgag gcttatcatg cagttatttt    420 

cgccatcttg gtattgtgga aataccgaca tccatagctt tggtgagctc tgttcaggtg    480 

gccaaattca gaaaaccatt acggaggaag aaggcgatgg ctaaaccagc gc            532 

 
           
             209  
             327  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            209 

cagcctcctg caaactttac tgttcatttc tacagtctca gtattttcct ttaaagagga     60 

tcttattggc tctggctctg gctctggctc tggctctggc tctggctctg gctctggctc    120 

tggctctggc tctggctctg gctctggctc tggctctggc tctggctctg gctctggctc    180 

tggctctggc tctggctctg gctctggctc tggctctggc aaaggagaac taaaatctaa    240 

tgaagtttca atattatatt catgattttt atggtctata tcagataatg aaatgggttg    300 

ttcattcaat gatgatacag acagatc                                        327 

 
           
             210  
             327  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            210 

gatctgtctg tatcatcatt gaatgaacaa cccatttcat tatctgatat agaccataaa     60 

aatcatgaat ataatattga aacttcatta gattttagtt ctcctttgcc agagccagag    120 

ccagagccag agccagagcc agagccagag ccagagccag agccagagcc agagccagag    180 

ccagagccag agccagagcc agagccagag ccagagccag agccagagcc agagccagag    240 

ccagagccag agccagagcc aataagatcc tctttaaagg aaaatactga gactgtagaa    300 

atgaacagta aagtttgcag gaggctg                                        327 

 
           
             211  
             280  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            211 

cagcctcccc caaactttac tgttcatttc tacagtctca gtattttcct ttaaagagga     60 

tcttattggc tctggctctg gctctggctc tggctctggc tctggctctg gctctggctc    120 

tggctctggc tctggctctg gctctggctc tggctctggc tctggctctg gcaaaggaga    180 

actaaaatct aatgaagttt caatattata ttcatgattt ttatggtcta tatcagataa    240 

tgaaatgggt tgttcattca atggatgata cagacagatc                          280 

 
           
             212  
             280  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            212 

gatctgtctg tatcatccat tgaatgaaca acccatttca ttatctgata tagaccataa     60 

aaatcatgaa tataatattg aaacttcatt agattttagt tctcctttgc cagagccaga    120 

gccagagcca gagccagagc cagagccaga gccagagcca gagccagagc cagagccaga    180 

gccagagcca gagccagagc cagagccaga gccaataaga tcctctttaa aggaaaatac    240 

tgagactgta gaaatgaaca gtaaagtttg ggggaggctg                          280 

 
           
             213  
             220  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            213 

gaccggcaat catcgggcca accacgatcc ccgcaatcag caacacaaag agcaataaca     60 

cttttagcat ggttattctc cttgcggcgc ggctgccgga gtatcagctt gcggtgcagg    120 

tgcaggtgca ggtgcaggtg caggtttagc ttccgttgtc cccgctgccg gttgtgccag    180 

caggttacgc acgcgagtct gcatcagttt ttccagcatc                          220 

 
           
             214  
             220  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            214 

gatgctggaa aaactgatgc agactcgcgt gcgtaacctg ctggcacaac cggcagcggg     60 

gacaacggaa gctaaacctg cacctgcacc tgcacctgca cctgcaccgc aagctgatac    120 

tccggcagcc gcgccgcaag gagaataacc atgctaaaag tgttattgct ctttgtgttg    180 

ctgattgcgg ggatcgtggt tggcccgatg attgccggtc                          220 

 
           
             215  
             220  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            215 

gaccggcaat catcgggcca accacgatcc ccgcaatcag caacacaaag agcaataaca     60 

cttttagcat ggttattctc cttgcggcgc ggctgccgga gtatcagctt gcggtgcagg    120 

tgcaggtgca ggtgcaggtg caggtttagc ttccgttgtc cccgctgccg gttgtgccag    180 

caggttacgc acgcgagtct gcatcagttt ttccagcatc                          220 

 
           
             216  
             220  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            216 

gatgctggaa aaactgatgc agactcgcgt gcgtaacctg ctggcacaac cggcagcggg     60 

gacaacggaa gctaaacctg cacctgcacc tgcacctgca cctgcaccgc aagctgatac    120 

tccggcagcc gcgccgcaag gagaataacc atgctaaaag tgttattgct ctttgtgttg    180 

ctgattgcgg ggatcgtggt tggcccgatg attgccggtc                          220 

 
           
             217  
             202  
             DNA  
             Escherichia coli K-12  
           
            217 

ggccggcaat catcgggcca accacgatcc ccgcaatcag caacacaaag agcaataaca     60 

cttttagcat ggttattctc cttgcggcgc ggctgccgga gtatcagctt gcggtgcagg    120 

tgcaggttta gcttccgttg tccccgctgc cggttgtgcc agcaggttac gcacgcgagt    180 

ctgcatcagt ttttccagca tc                                             202 

 
           
             218  
             202  
             DNA  
             Escherichia coli K-12  
           
            218 

gatgctggaa aaactgatgc agactcgcgt gcgtaacctg ctggcacaac cggcagcggg     60 

gacaacggaa gctaaacctg cacctgcacc gcaagctgat actccggcag ccgcgccgca    120 

aggagaataa ccatgctaaa agtgttattg ctctttgtgt tgctgattgc ggggatcgtg    180 

gttggcccga tgattgccgg cc                                             202 

 
           
             219  
             141  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            219 

ggcatcaata aaaggtaagc caagtttcgc cgccagtcgc cgggcaaaat caggaaccag     60 

gtcgaggtgc tttcaaaatc aggaaccagg tcgaggtgct ttaatgaagg aacgcagcat    120 

acccaggttg gttcaggatg c                                              141 

 
           
             220  
             141  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            220 

gcatcctgaa ccaacctggg tatgctgcgt tccttcatta aagcacctcg acctggttcc     60 

tgattttgaa agcacctcga cctggttcct gattttgccc ggcgactggc ggcgaaactt    120 

ggcttacctt ttattgatgc c                                              141 

 
           
             221  
             141  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            221 

ggcatcaata aaaggtaagc caagtttcgc cgccagtcgc cgggcaaaat caggaaccag     60 

gtcgaggtgc tttcaaaatc aggaaccagg tcgaggtgct ttaatgaagg aacgcagcat    120 

acccaggttg gttcaggatg c                                              141 

 
           
             222  
             141  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            222 

gcatcctgaa ccaacctggg tatgctgcgt tccttcatta aagcacctcg acctggttcc     60 

tgattttgaa agcacctcga cctggttcct gattttgccc ggcgactggc ggcgaaactt    120 

ggcttacctt ttattgatgc c                                              141 

 
           
             223  
             139  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            223 

gcagcaaacg ccacagtacc catgccagca atatcacact gatagccgcc agcaccgcca     60 

gcaccgccag caccgccagc agaatcggat ggtttgccag cgcataccac aagcgctcga    120 

accacggcag atgacctac                                                 139 

 
           
             224  
             139  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            224 

gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca tccgattctg     60 

ctggcggtgc tggcggtgct ggcggtgctg gcggctatca gtgtgatatt gctggcatgg    120 

gtactgtggc gtttgctgc                                                 139 

 
           
             225  
             139  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            225 

gcagcaaacg ccacagtacc catgccagca atatcacact gatagccgcc agcaccgcca     60 

gcaccgccag caccgccagc agaatcggat ggtttgccag cgcataccac aagcgctcga    120 

accacggcag atgacctac                                                 139 

 
           
             226  
             139  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            226 

gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca tccgattctg     60 

ctggcggtgc tggcggtgct ggcggtgctg gcggctatca gtgtgatatt gctggcatgg    120 

gtactgtggc gtttgctgc                                                 139 

 
           
             227  
             121  
             DNA  
             Escherichia coli K-12  
           
            227 

gcagcagacg ccacagtacc catgccagca atatcacact gatagccgcc agcaccgcca     60 

gcagaatcgg atggtttgcc agcgcatacc acacgcgctc gaaccacggc agatgaccta    120 

c                                                                    121 

 
           
             228  
             121  
             DNA  
             Escherichia coli K-12  
           
            228 

gtaggtcatc tgccgtggtt cgagcgcgtg tggtatgcgc tggcaaacca tccgattctg     60 

ctggcggtgc tggcggctat cagtgtgata ttgctggcat gggtactgtg gcgtctgctg    120 

c                                                                    121 

 
           
             229  
             237  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            229 

gtcgctgata atattctctt ttcgtcatcc cactgttacg gccttcggtt acgtttgcgg     60 

taaattgagc catacccatg cacatttgat ctttattgtt attcatttga tctttattgt    120 

tattcgtttt atccgggtgg agagcgcctt ccaacacttt atcaccgctg atggcatgtt    180 

gcgcccacgc tgaattgtta gcgataaact ttttggagcc gatggcaata ccgtatt       237 

 
           
             230  
             237  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            230 

aatacggtat tgccatcggc tccaaaaagt ttatcgctaa caattcagcg tgggcgcaac     60 

atgccatcag cggtgataaa gtgttggaag gcgctctcca cccggataaa acgaataaca    120 

ataaagatca aatgaataac aataaagatc aaatgtgcat gggtatggct caatttaccg    180 

caaacgtaac cgaaggccgt aacagtggga tgacgaaaag agaatattat cagcgac       237 

 
           
             231  
             237  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            231 

gtcgctgata atattctctt ttcgtcatcc cactgttacg gccttcggtt acgtttgcgg     60 

taaattgagc catacccatg cacatttgat ctttattgtt attcatttga tctttattgt    120 

tattcgtttt atccgggtgg agagcgcctt ccaacacttt atcaccgctg atggcatgtt    180 

gcgcccacgc tgaattgtta gcgataaact ttttggagcc gatggcaata ccgtatt       237 

 
           
             232  
             237  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            232 

aatacggtat tgccatcggc tccaaaaagt ttatcgctaa caattcagcg tgggcgcaac     60 

atgccatcag cggtgataaa gtgttggaag gcgctctcca cccggataaa acgaataaca    120 

ataaagatca aatgaataac aataaagatc aaatgtgcat gggtatggct caatttaccg    180 

caaacgtaac cgaaggccgt aacagtggga tgacgaaaag agaatattat cagcgac       237 
 
           
             233  
             168  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            233 

gcagttgctc ggttttaaca ttgcagtgat gacttatatc tttatcttta tctttatctt     60 

tatctttatc tttatcttta tctttaacag ttaatacgct ttcattatca tcttgtcgat    120 

taaatattat ttttagatcg ccatcgtcaa actcatgtaa accatttc                 168 

 
           
             234  
             168  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            234 

gaaatggttt acatgagttt gacgatggcg atctaaaaat aatatttaat cgacaagatg     60 

ataatgaaag cgtattaact gttaaagata aagataaaga taaagataaa gataaagata    120 

aagataaaga tataagtcat cactgcaatg ttaaaaccga gcaactgc                 168 

 
           
             235  
             156  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            235 

gcagttgctc ggttttaaca ttgcagtgat gacttatatc tttatcttta tctttatctt     60 

tatctttatc tttaacagtt aatacgcttt cattatcatc ttgtcgatta aatattattt    120 

ttagatcgcc atcgtcaaac tcatgtaaac catttc                              156 

 
           
             236  
             156  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            236 

gaaatggttt acatgagttt gacgatggcg atctaaaaat aatatttaat cgacaagatg     60 

ataatgaaag cgtattaact gttaaagata aagataaaga taaagataaa gataaagata    120 

taagtcatca ctgcaatgtt aaaaccgagc aactgc                              156 

 
           
             237  
             171  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            237 

gactggcgat gaagagcgtt ttaatgagtt tatcagtgaa ctaaccagtc gaatgcctca     60 

acacagagag cgaataatga cgattgcaga gcgaattcat aatgacgatt gcagagcgaa    120 

ttcataatga tggatggctg ttgggaaggg aagaagggga acagcgcatt c             171 

 
           
             238  
             171  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            238 

gaatgcgctg ttccccttct tcccttccca acagccatcc atcattatga attcgctctg     60 

caatcgtcat tatgaattcg ctctgcaatc gtcattattc gctctctgtg ttgaggcatt    120 

cgactggtta gttcactgat aaactcatta aaacgctctt catcgccagt c             171 

 
           
             239  
             171  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            239 

gactggcgat gaagagcgtt ttaatgagtt tatcagtgaa ctaaccagtc gaatgcctca     60 

acacagagag cgaataatga cgattgcaga gcgaattcat aatgacgatt gcagagcgaa    120 

ttcataatga tggatggctg ttgggaaggg aagaagggga acagcgcatt c             171 

 
           
             240  
             171  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            240 

gaatgcgctg ttccccttct tcccttccca acagccatcc atcattatga attcgctctg     60 

caatcgtcat tatgaattcg ctctgcaatc gtcattattc gctctctgtg ttgaggcatt    120 

cgactggtta gttcactgat aaactcatta aaacgctctt catcgccagt c             171 

 
           
             241  
             296  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            241 

gcagtgatca ttattagcac cgctttctgg atgttctggc gtacctggcg cggcgaacgc     60 

aactggctgg agaatatgca tgagcatcat caccacgatc acgaacatca tcaagaccac    120 

gaccacgacc acgaccacga acatcatcac catcatgaac atggcgacaa cgaagagtat    180 

caggatgccc atgcacgagc ccatgccaat gacattaaac gacgctttga tggtagagag    240 

gtcaccaact ggcaaatttt gttatttggc ttaacaggtg gccttattcc ctgccc        296 

 
           
             242  
             296  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            242 

gggcagggaa taaggccacc tgttaagcca aataacaaaa tttgccagtt ggtgacctct     60 

ctaccatcaa agcgtcgttt aatgtcattg gcatgggctc gtgcatgggc atcctgatac    120 

tcttcgttgt cgccatgttc atgatggtga tgatgttcgt ggtcgtggtc gtggtcgtgg    180 

tcttgatgat gttcgtgatc gtggtgatga tgctcatgca tattctccag ccagttgcgt    240 

tcgccgcgcc aggtacgcca gaacatccag aaagcggtgc taataatgat cactgc        296 
 
           
             243  
             308  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            243 

gcagtgatca ttattagcac cgctttctgg atgttctggc gtacctggcg cggcgaacgc     60 

aactggctgg agaatatgca tgagcatcat caccacgatc acgaacatca tcaagaccac    120 

gaccacgacc acgaccacga ccacgaccac gaacatcatc accatcatga acatggcgac    180 

aacgaagagt atcaggatgc ccatgcacga gcccatgcca atgacattaa acgacgcttt    240 

gatggtagag aggtcaccaa ctggcaaatt ttgttatttg gcttaacagg tggccttatt    300 

ccctgccc                                                             308 

 
           
             244  
             308  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            244 

gggcagggaa taaggccacc tgttaagcca aataacaaaa tttgccagtt ggtgacctct     60 

ctaccatcaa agcgtcgttt aatgtcattg gcatgggctc gtgcatgggc atcctgatac    120 

tcttcgttgt cgccatgttc atgatggtga tgatgttcgt ggtcgtggtc gtggtcgtgg    180 

tcgtggtcgt ggtcttgatg atgttcgtga tcgtggtgat gatgctcatg catattctcc    240 

agccagttgc gttcgccgcg ccaggtacgc cagaacatcc agaaagcggt gctaataatg    300 

atcactgc                                                             308 

 
           
             245  
             281  
             DNA  
             Escherichia coli K-12  
           
            245 

gcagtgatca ttattagcac cgcgttctgg atgttctggc gtacctggcg cggcgaacgc     60 

aactggctgg agaatatgca cgggcatgat tatgagcatc atcatcacga tcacgaacat    120 

caccacgacc atggacatca tcaccatcac gaacatggcg agtatcagga tgcccatgca    180 

cgagcccatg ccaatgacat taaacgacgc tttgatggta gagaggtcac caactggcaa    240 

attttgttat ttggcttaac cggtggcctt atcccctgcc c                        281 

 
           
             246  
             281  
             DNA  
             Escherichia coli K-12  
           
            246 

gggcagggga taaggccacc ggttaagcca aataacaaaa tttgccagtt ggtgacctct     60 

ctaccatcaa agcgtcgttt aatgtcattg gcatgggctc gtgcatgggc atcctgatac    120 

tcgccatgtt cgtgatggtg atgatgtcca tggtcgtggt gatgttcgtg atcgtgatga    180 

tgatgctcat aatcatgccc gtgcatattc tccagccagt tgcgttcgcc gcgccaggta    240 

cgccagaaca tccagaacgc ggtgctaata atgatcactg c                        281 

 
           
             247  
             134  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            247 

gccggaggag ggtgatgagc ggttatattt agtgtgcgaa taattttgct tgcaaatgca     60 

aatgcaaatg caaatgagaa atatacgcat tcatatttgt cgtgtaaacc aaacagagaa    120 

tgtcttttca gcgc                                                      134 

 
           
             248  
             134  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            248 

gcgctgaaaa gacattctct gtttggttta cacgacaaat atgaatgcgt atatttctca     60 

tttgcatttg catttgcatt tgcaagcaaa attattcgca cactaaatat aaccgctcat    120 

caccctcctc cggc                                                      134 

 
           
             249  
             142  
             DNA  
             Escherichia coli O157H7 EDL933  
             
               misc_feature  
               (1)...(142)  
               n = A,T,C or G  
             
           
            249 

gccggaggag ggtgatgagc ggttatattt agtgtgcgaa taatttkgct tgcaaatgca     60 

aatgcaaatt gcaaatgcaa atgagaaata tacgcattca tatttgtcng tgtaaaccaa    120 

acagagaatg tcttttcagc gc                                             142 

 
           
             250  
             142  
             DNA  
             Escherichia coli O157H7 EDL933  
             
               misc_feature  
               (1)...(142)  
               n = A,T,C or G  
             
           
            250 

gcgctgaaaa gacattctct gtttggttta cacngacaaa tatgaatgcg tatatttctc     60 

atttgcattt gcaatttgca tttgcatttg caagcmaaat tattcgcaca ctaaatataa    120 

ccgctcatca ccctcctccg gc                                             142 

 
           
             251  
             235  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            251 

gctctccatg gtatcttctg acccaggggt atctacattg ccctgtgcag gtgtattatt     60 

tgcaggctta ttctctaccg tacgtgcgct tgtagttgta gttgtagtag ttgttgttgt    120 

tgtttgttct accggctgat tttttcgatg aagcgcagcg gtgacggcaa caccaattcc    180 

cccaccaaga atcaatgcgc cactaagacc gtagccagcc cccgatgaaa ctttc         235 

 
           
             252  
             235  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            252 

gaaagtttca tcgggggctg gctacggtct tagtggcgca ttgattcttg gtgggggaat     60 

tggtgttgcc gtcaccgctg cgcttcatcg aaaaaatcag ccggtagaac aaacaacaac    120 

aacaactact acaactacaa ctacaagcgc acgtacggta gagaataagc ctgcaaataa    180 

tacacctgca cagggcaatg tagatacccc tgggtcagaa gataccatgg agagc         235 

 
           
             253  
             235  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            253 

gctctccatg gtatcttctg acccaggggt atctacattg ccctgtgcag gtgtattatt     60 

tgcaggctta ttctctaccg tacgtgcgct tgtagttgta gttgtagtag ttgttgttgt    120 

tgtttgttct accggctgat tttttcgatg aagcgcagcg gtgacggcaa caccaattcc    180 

cccaccaaga atcaatgcgc cactaagacc gtagccagcc cccgatgaaa ctttc         235 

 
           
             254  
             235  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            254 

gaaagtttca tcgggggctg gctacggtct tagtggcgca ttgattcttg gtgggggaat     60 

tggtgttgcc gtcaccgctg cgcttcatcg aaaaaatcag ccggtagaac aaacaacaac    120 

aacaactact acaactacaa ctacaagcgc acgtacggta gagaataagc ctgcaaataa    180 

tacacctgca cagggcaatg tagatacccc tgggtcagaa gataccatgg agagc         235 

 
           
             255  
             128  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            255 

gttgccgacc cacagcgata cgccattggt taatacgtta ctcaatgatt tcaatgattt     60 

caatgatgaa atgcgtaaac tcgcgccgct ttctgccgct ctggagcaaa gcgatctggc    120 

aatcagct                                                             128 

 
           
             256  
             128  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            256 

agctgattgc cagatcgctt tgctccagag cggcagaaag cggcgcgagt ttacgcattt     60 

catcattgaa atcattgaaa tcattgagta acgtattaac caatggcgta tcgctgtggg    120 

tcggcaac                                                             128 
 
           
             257  
             128  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            257 

gttgccgacc cacagcgata cgccattggt taatacgtta ctcaatgatt tcaatgattt     60 

caatgatgaa atgcgtaaac tcgcgccgct ttctgccgct ctggagcaaa gcgatctggc    120 

aatcagct                                                             128 

 
           
             258  
             128  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            258 

agctgattgc cagatcgctt tgctccagag cggcagaaag cggcgcgagt ttacgcattt     60 

catcattgaa atcattgaaa tcattgagta acgtattaac caatggcgta tcgctgtggg    120 

tcggcaac                                                             128 

 
           
             259  
             123  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            259 

gccgaaaaac gatgcagctg acttaggcgc tgctggcggc atgggtggca tgggtggcat     60 

gggcggcatg atgtaattgc cctgcacctc gcagaaataa acaaaccccc gggcagaaat    120 

gtc                                                                  123 

 
           
             260  
             123  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            260 

gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta catcatgccg     60 

cccatgccac ccatgccacc catgccgcca gcagcgccta agtcagctgc atcgtttttc    120 

ggc                                                                  123 

 
           
             261  
             123  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            261 

gccgaaaaac gatgcagctg acttaggcgc tgctggcggc atgggtggca tgggtggcat     60 

gggcggcatg atgtaattgc cctgcacctc gcagaaataa acaaaccccc gggcagaaat    120 

gtc                                                                  123 

 
           
             262  
             123  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            262 

gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta catcatgccg     60 

cccatgccac ccatgccacc catgccgcca gcagcgccta agtcagctgc atcgtttttc    120 

ggc                                                                  123 

 
           
             263  
             210  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            263 

gtgaaggata agctgcattt gtcagtgatg tccgaagtta accgtcaggt tatgcgtctg     60 

caaacagaga tggcttaacc aaagtgctat gcagtaaaaa gtgctatgca gtaaaaagtg    120 

ctatgcagta ataagacggc tcctgattca ggagccgttg atgtttatgg gggttacgcg    180 

acgctttctt ctttatcttt agcgtcaggc                                     210 

 
           
             264  
             210  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            264 

gcctgacgct aaagataaag aagaaagcgt cgcgtaaccc ccataaacat caacggctcc     60 

tgaatcagga gccgtcttat tactgcatag cactttttac tgcatagcac tttttactgc    120 

atagcacttt ggttaagcca tctctgtttg cagacgcata acctgacggt taacttcgga    180 

catcactgac aaatgcagct tatccttcac                                     210 

 
           
             265  
             210  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            265 

gtgaaggata agctgcattt gtcagtgatg tccgaagtta accgtcaggt tatgcgtctg     60 

caaacagaga tggcttaacc aaagtgctat gcagtaaaaa gtgctatgca gtaaaaagtg    120 

ctatgcagta ataagacggc tcctgattca ggagccgttg atgtttatgg gggttacgcg    180 

acgctttctt ctttatcttt agcgtcaggc                                     210 

 
           
             266  
             210  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            266 

gcctgacgct aaagataaag aagaaagcgt cgcgtaaccc ccataaacat caacggctcc     60 

tgaatcagga gccgtcttat tactgcatag cactttttac tgcatagcac tttttactgc    120 

atagcacttt ggttaagcca tctctgtttg cagacgcata acctgacggt taacttcgga    180 

catcactgac aaatgcagct tatccttcac                                     210 

 
           
             267  
             259  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            267 

acaaggttct ggcgtgttac caacgcgaag ctaacaagga aatagctcgt ctattaaata     60 

atcatcagaa gttaaataat ctacagaagt taaataatct acagaagtta aataatctac    120 

agaagttaaa taatatacag aagttaaata atatacagga gttaaataat tcgcaggagt    180 

taaataattc gcaggagtta aataattcgc aggagttaaa taactcgcag gacttaaaaa    240 

attcgcaggt gagttgtaa                                                 259 

 
           
             268  
             259  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            268 

ttacaactca cctgcgaatt ttttaagtcc tgcgagttat ttaactcctg cgaattattt     60 

aactcctgcg aattatttaa ctcctgcgaa ttatttaact cctgtatatt atttaacttc    120 

tgtatattat ttaacttctg tagattattt aacttctgta gattatttaa cttctgtaga    180 

ttatttaact tctgatgatt atttaataga cgagctattt ccttgttagc ttcgcgttgg    240 

taacacgcca gaaccttgt                                                 259 

 
           
             269  
             277  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            269 

acaaggttct ggcgtgttac caacgcgaag ctaacaagga aatagctcgt ctattaaata     60 

atcatcagaa gttaaataat ctacagaagt taaataatct acagaagtta aataatctac    120 

agaagttaaa taatctacag aagttaaata atatacagaa gttaaataat atacaggagt    180 

taaataattc gcaggagtta aataattcgc aggagttaaa taattcgcag gagttaaata    240 

actcgcagga cttaaaaaat tcgcaggtga gttgtaa                             277 

 
           
             270  
             277  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            270 

ttacaactca cctgcgaatt ttttaagtcc tgcgagttat ttaactcctg cgaattattt     60 

aactcctgcg aattatttaa ctcctgcgaa ttatttaact cctgtatatt atttaacttc    120 

tgtatattat ttaacttctg tagattattt aacttctgta gattatttaa cttctgtaga    180 

ttatttaact tctgtagatt atttaacttc tgatgattat ttaatagacg agctatttcc    240 

ttgttagctt cgcgttggta acacgccaga accttgt                             277 

 
           
             271  
             172  
             DNA  
             Escherichia coli O157H7 pO157  
           
            271 

ggcgtccttc atcggcctgt ccgttaaact caggctacct cacacctcac acctcacacc     60 

tcacacctca cacctcacac ctcacacctc acacctcaca cctcacaaca gcgggtctgg    120 

acagaaagcg gctcttgatg tggattatcg catggtgtgg gctttcagcg gc            172 

 
           
             272  
             172  
             DNA  
             Escherichia coli O157H7 pO157  
           
            272 

gccgctgaaa gcccacacca tgcgataatc cacatcaaga gccgctttct gtccagaccc     60 

gctgttgtga ggtgtgaggt gtgaggtgtg aggtgtgagg tgtgaggtgt gaggtgtgag    120 

gtgtgaggtg tgaggtagcc tgagtttaac ggacaggccg atgaaggacg cc            172 

 
           
             273  
             184  
             DNA  
             Escherichia coli O157H7 pO157  
           
            273 

gccgcccctt acattacgcg gacattctgc tgtgggcttc tgtcttttca gacctgtcat     60 

ttgtctgtta ctgatgatgt tgttgttcat gctactgcta ctgctactgc tactgctact    120 

gctactgcta ctgttcttct tttttctgct gctctgatta ctgtctgttt tgttgttctc    180 

ctgc                                                                 184 

 
           
             274  
             184  
             DNA  
             Escherichia coli O157H7 pO157  
           
            274 

gcaggagaac aacaaaacag acagtaatca gagcagcaga aaaaagaaga acagtagcag     60 

tagcagtagc agtagcagta gcagtagcag tagcatgaac aacaacatca tcagtaacag    120 

acaaatgaca ggtctgaaaa gacagaagcc cacagcagaa tgtccgcgta atgtaagggg    180 

cggc                                                                 184 

 
           
             275  
             141  
             DNA  
             Escherichia coli O157H7 pOSAK1  
           
            275 

gggtttgttt tcagtgaagt attcgccaag gttctttgat gatgatggat tgcaaacatc     60 

attaagtatt tgatatataa agccttctat ggctcttaat gcagagaagc agtatgttga    120 

gtaatcttcc atttcgacat c                                              141 

 
           
             276  
             141  
             DNA  
             Escherichia coli O157H7 pOSAK1  
           
            276 

gatgtcgaaa tggaagatta ctcaacatac tgcttctctg cattaagagc catagaaggc     60 

tttatatatc aaatacttaa tgatgtttgc aatccatcat catcaaagaa ccttggcgaa    120 

tacttcactg aaaacaaacc c                                              141 

 
           
             277  
             173  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            277 

gtttcgggtg aatagagggc gcttttctcg ttaattttga ttattaatca gtttgttatg     60 

ttatgttatg ttgcgagtaa aaaaatagca tctgactttc aatattggtg atccataaaa    120 

caatattgaa aatttctttt tgctacgccg tgttttcaat attggtgagg aac           173 

 
           
             278  
             173  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            278 

gttcctcacc aatattgaaa acacggcgta gcaaaaagaa attttcaata ttgttttatg     60 

gatcaccaat attgaaagtc agatgctatt tttttactcg caacataaca taacataaca    120 

aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aac           173 

 
           
             279  
             173  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            279 

gtttcgggtg aatagagggc gcttttctcg ttaattttga ttattaatca gtttgttatg     60 

ttatgttatg ttgcgagtaa aaaaatagca tctgactttc aatattggtg atccataaaa    120 

caatattgaa aatttctttt tgctacgccg tgttttcaat attggtgagg aac           173 

 
           
             280  
             173  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            280 

gttcctcacc aatattgaaa acacggcgta gcaaaaagaa attttcaata ttgttttatg     60 

gatcaccaat attgaaagtc agatgctatt tttttactcg caacataaca taacataaca    120 

aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aac           173 

 
           
             281  
             134  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            281 

gcctgcggct gggcaaattc gttcctgacg aatttgtcac tcgcttgccg ccttcctgca     60 

acttgcaact tgaattattt agagtataag tattacgcca gctcgaccat tttgttgtcg    120 

atcaggcgag catc                                                      134 

 
           
             282  
             134  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            282 

gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttata ctctaaataa     60 

ttcaagttgc aagttgcagg aaggcggcaa gcgagtgaca aattcgtcag gaacgaattt    120 

gcccagccgc aggc                                                      134 

 
           
             283  
             134  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            283 

gcctgcggct gggcaaattc gttcctgacg aatttgtcac tcgcttgccg ccttcctgca     60 

acttgcaact tgaattattt agagtataag tattacgcca gctcgaccat tttgttgtcg    120 

atcaggcgag catc                                                      134 

 
           
             284  
             134  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            284 

gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttata ctctaaataa     60 

ttcaagttgc aagttgcagg aaggcggcaa gcgagtgaca aattcgtcag gaacgaattt    120 

gcccagccgc aggc                                                      134 

 
           
             285  
             269  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            285 

gttcttcata cagcgtccac gtcgggcctt tccaccagat ccataccagc agaaaaatcc     60 

acgccagtat cagcaagcag aaaaatccac gccagtatca gcagccagaa agcggagact    120 

ttaaaccggg gcatcgccgg tcgcagggct gatttgagtc cgctgagtag tcggggtgtt    180 

ggtaatctga acacaaaaac tcctgttctc ttttattgtt ctgccgtgaa tttttgttca    240 

agctgcgtaa gtaatgatgg ctcccagtc                                      269 

 
           
             286  
             269  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            286 

gactgggagc catcattact tacgcagctt gaacaaaaat tcacggcaga acaataaaag     60 

agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg actcaaatca    120 

gccctgcgac cggcgatgcc ccggtttaaa gtctccgctt tctggctgct gatactggcg    180 

tggatttttc tgcttgctga tactggcgtg gatttttctg ctggtatgga tctggtggaa    240 

aggcccgacg tggacgctgt atgaagaac                                      269 

 
           
             287  
             269  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            287 

gttcttcata cagcgtccac gtcgggcctt tccaccagat ccataccagc agaaaaatcc     60 

acgccagtat cagcaagcag aaaaatccac gccagtatca gcagccagaa agcggagact    120 

ttaaaccggg gcatcgccgg tcgcagggct gatttgagtc cgctgagtag tcggggtgtt    180 

ggtaatctga acacaaaaac tcctgttctc ttttattgtt ctgccgtgaa tttttgttca    240 

agctgcgtaa gtaatgatgg ctcccagtc                                      269 

 
           
             288  
             269  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            288 

gactgggagc catcattact tacgcagctt gaacaaaaat tcacggcaga acaataaaag     60 

agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg actcaaatca    120 

gccctgcgac cggcgatgcc ccggtttaaa gtctccgctt tctggctgct gatactggcg    180 

tggatttttc tgcttgctga tactggcgtg gatttttctg ctggtatgga tctggtggaa    240 

aggcccgacg tggacgctgt atgaagaac                                      269 

 
           
             289  
             235  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            289 

gcccgccggg ccgatgacca tataccacgg taactgccac aggaaacggc ggttgtcgag     60 

atggcgttgc aggcgcagca gccagcggtc aaggtaacgc tgctgggcgt tcagctccac    120 

actgagcgga tcaatggcct cttcgcgctg ctgtttttgc tgtttttcaa gcagttgcag    180 

gcgcttcatt acccggacgg taagccacac cagggcaata atcccccacg ccgcc         235 

 
           
             290  
             235  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            290 

ggcggcgtgg gggattattg ccctggtgtg gcttaccgtc cgggtaatga agcgcctgca     60 

actgcttgaa aaacagcaaa aacagcagcg cgaagaggcc attgatccgc tcagtgtgga    120 

gctgaacgcc cagcagcgtt accttgaccg ctggctgctg cgcctgcaac gccatctcga    180 

caaccgccgt ttcctgtggc agttaccgtg gtatatggtc atcggcccgg cgggc         235 

 
           
             291  
             235  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            291 

gcccgccggg ccgatgacca tataccacgg taactgccac aggaaacggc ggttgtcgag     60 

atggcgttgc aggcgcagca gccagcggtc aaggtaacgc tgctgggcgt tcagctccac    120 

actgagcgga tcaatggcct cttcgcgctg ctgtttttgc tgtttttcaa gcagttgcag    180 

gcgcttcatt acccggacgg taagccacac cagggcaata atcccccacg ccgcc         235 

 
           
             292  
             235  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            292 

ggcggcgtgg gggattattg ccctggtgtg gcttaccgtc cgggtaatga agcgcctgca     60 

actgcttgaa aaacagcaaa aacagcagcg cgaagaggcc attgatccgc tcagtgtgga    120 

gctgaacgcc cagcagcgtt accttgaccg ctggctgctg cgcctgcaac gccatctcga    180 

caaccgccgt ttcctgtggc agttaccgtg gtatatggtc atcggcccgg cgggc         235 

 
           
             293  
             162  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            293 

gggactggat attgtgcagg gttcagcagg ggtgctgata ggtgcgccga cgggcgtggc     60 

ctgctcggtg tgtccgggag ggattaccta tgctaacccg gtgaacccgg tgctgggtgc    120 

gaaggtgctg ccgggcgaga cggaccttgc gctgcccggc cc                       162 

 
           
             294  
             162  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            294 

gggccgggca gcgcaaggtc cgtctcgccc ggcagcacct tcgcacccag caccgggttc     60 

accgggttag cataggtaat ccctcccgga cacaccgagc aggccacgcc cgtcggcgca    120 

cctatcagca cccctgctga accctgcaca atatccagtc cc                       162 

 
           
             295  
             162  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            295 

gggactggat attgtgcagg gttcagcagg ggtgctgata ggtgcgccga cgggcgtggc     60 

ctgctcggtg tgtccgggag ggattaccta tgctaacccg gtgaacccgg tgctgggtgc    120 

gaaggtgctg ccgggcgaga cggaccttgc gctgcccggc cc                       162 

 
           
             296  
             162  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            296 

gggccgggca gcgcaaggtc cgtctcgccc ggcagcacct tcgcacccag caccgggttc     60 

accgggttag cataggtaat ccctcccgga cacaccgagc aggccacgcc cgtcggcgca    120 

cctatcagca cccctgctga accctgcaca atatccagtc cc                       162 

 
           
             297  
             198  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            297 

aacactttgt tccacaagaa aattgtcagg gagaatggcg gagtggcgaa aaatataaat     60 

agaggtgtac tgattaacaa attgataaaa taaaataaaa ttaaaatctt ctctgatata    120 

agaaaataaa agcatagacc gtagaatggg gggcatttcg tggcgaaaaa agagcaatgc    180 

caattttatg cacataat                                                  198 

 
           
             298  
             198  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            298 

attatgtgca taaaattggc attgctcttt tttcgccacg aaatgccccc cattctacgg     60 

tctatgcttt tattttctta tatcagagaa gattttaatt ttattttatt ttatcaattt    120 

gttaatcagt acacctctat ttatattttt cgccactccg ccattctccc tgacaatttt    180 

cttgtggaac aaagtgtt                                                  198 

 
           
             299  
             198  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            299 

aacactttgt tccacaagaa aattgtcagg gagaatggcg gagtggcgaa aaatataaat     60 

agaggtgtac tgattaacaa attgataaaa taaaataaaa ttaaaatctt ctctgatata    120 

agaaaataaa agcatagacc gtagaatggg gggcatttcg tggcgaaaaa agagcaatgc    180 

caattttatg cacataat                                                  198 

 
           
             300  
             198  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            300 

attatgtgca taaaattggc attgctcttt tttcgccacg aaatgccccc cattctacgg     60 

tctatgcttt tattttctta tatcagagaa gattttaatt ttattttatt ttatcaattt    120 

gttaatcagt acacctctat ttatattttt cgccactccg ccattctccc tgacaatttt    180 

cttgtggaac aaagtgtt                                                  198 

 
           
             301  
             254  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            301 

gcggcgcatt agcgtcgtat caggcaatca ataatgtcgg atatgaaaag cggaaacata     60 

tcgatgaaag cgatcttgat cccattttta tctcttctga ttccgttaac cccgcaatct    120 

gcattcgctc agagtgagcc ggagccggag ctgaagctgg aaagtgtggt gattgtcagt    180 

cgtcatggtg tgcgtgcccc aaccaaggcc acgcaactga tgcaggatgt caccccagac    240 

gcatggccaa actg                                                      254 

 
           
             302  
             254  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            302 

cagtttggcc atgcgtctgg ggtgacatcc tgcatcagtt gcgtggcctt ggttggggca     60 

cgcacaccat gacgactgac aatcaccaca ctttccagct tcagctccgg ctccggctca    120 

ctctgagcga atgcagattg cggggttaac ggaatcagaa gagataaaaa tgggatcaag    180 

atcgctttca tcgatatgtt tccgcttttc atatccgaca ttattgattg cctgatacga    240 

cgctaatgcg ccgc                                                      254 

 
           
             303  
             254  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            303 

gcggcgcatt agcgtcgtat caggcaatca ataatgtcgg atatgaaaag cggaaacata     60 

tcgatgaaag cgatcttgat cccattttta tctcttctga ttccgttaac cccgcaatct    120 

gcattcgctc agagtgagcc ggagccggag ctgaagctgg aaagtgtggt gattgtcagt    180 

cgtcatggtg tgcgtgcccc aaccaaggcc acgcaactga tgcaggatgt caccccagac    240 

gcatggccaa actg                                                      254 

 
           
             304  
             254  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            304 

cagtttggcc atgcgtctgg ggtgacatcc tgcatcagtt gcgtggcctt ggttggggca     60 

cgcacaccat gacgactgac aatcaccaca ctttccagct tcagctccgg ctccggctca    120 

ctctgagcga atgcagattg cggggttaac ggaatcagaa gagataaaaa tgggatcaag    180 

atcgctttca tcgatatgtt tccgcttttc atatccgaca ttattgattg cctgatacga    240 

cgctaatgcg ccgc                                                      254 

 
           
             305  
             235  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            305 

gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg     60 

cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc    120 

gtcgaaaatg attgccggat tgccggatca tgtacacaac gcggcgcgta ttcgttatcc    180 

gatggtacgc gtggactggc tgcgtaagcg ccatctgagc gacacctccc agcgc         235 

 
           
             306  
             235  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            306 

gcgctgggag gtgtcgctca gatggcgctt acgcagccag tccacgcgta ccatcggata     60 

acgaatacgc gccgcgttgt gtacatgatc cggcaatccg gcaatcattt tcgacggata    120 

tttatccagt tcgaacggtt ttgccgccac aaagcgacca tccttcaccg tcgcgcggat    180 

agccccccag tgcgacccgg taagaatgcc ctctttcgag atgacagcct cagtc         235 

 
           
             307  
             235  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            307 

gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg     60 

cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc    120 

gtcgaaaatg attgccggat tgccggatca tgtacacaac gcggcgcgta ttcgttatcc    180 

gatggtacgc gtggactggc tgcgtaagcg ccatctgagc gacacctccc agcgc         235 

 
           
             308  
             235  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            308 

gcgctgggag gtgtcgctca gatggcgctt acgcagccag tccacgcgta ccatcggata     60 

acgaatacgc gccgcgttgt gtacatgatc cggcaatccg gcaatcattt tcgacggata    120 

tttatccagt tcgaacggtt ttgccgccac aaagcgacca tccttcaccg tcgcgcggat    180 

agccccccag tgcgacccgg taagaatgcc ctctttcgag atgacagcct cagtc         235 
 
           
             309  
             269  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            309 

gtttgctgta gcccaggccg ttgatcttct tcactttgat ctgcacagga atacgctctt     60 

tcatcgcttc aacgtggcta atcacaccga tggttttgcc actggcgttc agggcatcca    120 

gcgcatcaag ggcggtatcc agcgtttcgc tatccagcgt gccaaaacct tcatcaagga    180 

acagcgagtc aatacgtgtt ttatggctga ccagatccga aagcgccagc gccagcgcca    240 

gactaacgag gaaactttcg ccgccggaa                                      269 
 
           
             310  
             269  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            310 

ttccggcggc gaaagtttcc tcgttagtct ggcgctggcg ctggcgcttt cggatctggt     60 

cagccataaa acacgtattg actcgctgtt ccttgatgaa ggttttggca cgctggatag    120 

cgaaacgctg gataccgccc ttgatgcgct ggatgccctg aacgccagtg gcaaaaccat    180 

cggtgtgatt agccacgttg aagcgatgaa agagcgtatt cctgtgcaga tcaaagtgaa    240 

gaagatcaac ggcctgggct acagcaaac                                      269 
 
           
             311  
             269  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            311 

gtttgctgta gcccaggccg ttgatcttct tcactttgat ctgcacagga atacgctctt     60 

tcatcgcttc aacgtggcta atcacaccga tggttttgcc actggcgttc agggcatcca    120 

gcgcatcaag ggcggtatcc agcgtttcgc tatccagcgt gccaaaacct tcatcaagga    180 

acagcgagtc aatacgtgtt ttatggctga ccagatccga aagcgccagc gccagcgcca    240 

gactaacgag gaaactttcg ccgccggaa                                      269 

 
           
             312  
             269  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            312 

ttccggcggc gaaagtttcc tcgttagtct ggcgctggcg ctggcgcttt cggatctggt     60 

cagccataaa acacgtattg actcgctgtt ccttgatgaa ggttttggca cgctggatag    120 

cgaaacgctg gataccgccc ttgatgcgct ggatgccctg aacgccagtg gcaaaaccat    180 

cggtgtgatt agccacgttg aagcgatgaa agagcgtatt cctgtgcaga tcaaagtgaa    240 

gaagatcaac ggcctgggct acagcaaac                                      269 

 
           
             313  
             270  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            313 

gacttactca gcgccgccaa cgaagtccag attctggcct tctttcaggg tgacgtaagc     60 

ttttttccag tcgctacgac gaccgatacg ctgtccgtga cgtttaactt tccctttaac    120 

taccagggtg ttaacgactt cgacttcgac ttcaaacagt ttctgcacag cagctttgat    180 

ttctgctttg gtcgcgtctt tagcaacttt gagtacgatg gtgttggatt tttccatcgc    240 

agtagacgct ttttcagaaa cgtgcggtgc                                     270 

 
           
             314  
             270  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            314 

gcaccgcacg tttctgaaaa agcgtctact gcgatggaaa aatccaacac catcgtactc     60 

aaagttgcta aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa    120 

gtcgaagtcg aagtcgttaa caccctggta gttaaaggga aagttaaacg tcacggacag    180 

cgtatcggtc gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat    240 

ctggacttcg ttggcggcgc tgagtaagtc                                     270 

 
           
             315  
             270  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            315 

gacttactca gcgccgccaa cgaagtccag attctggcct tctttcaggg tgacgtaagc     60 

ttttttccag tcgctacgac gaccgatacg ctgtccgtga cgtttaactt tccctttaac    120 

taccagggtg ttaacgactt cgacttcgac ttcaaacagt ttctgcacag cagctttgat    180 

ttctgctttg gtcgcgtctt tagcaacttt gagtacgatg gtgttggatt tttccatcgc    240 

agtagacgct ttttcagaaa cgtgcggtgc                                     270 
 
           
             316  
             270  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            316  
           
             317  
             269  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            317 

gaaaatccgg cgacggttgc cagactccag tcatcggaaa tttcccgatc ggttcgctca     60 

ggccgtccag gtattcaggt tcggctgtct gcaacgacgc gtcattcaac tcaccggtcg    120 

tagcactctc gcgcatgcgc gcgttatgtg tggggtttaa ttctttatct gtatctgtat    180 

ctgtcgtgat ttgtcgtgac atatgcgtga cgcgtcgtga ctcatcgtga caatcagtgt    240 

tctgcttccg cagtctttcc cgctcccgc                                      269 

 
           
             318  
             269  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            318 

gcgggagcgg gaaagactgc ggaagcagaa cactgattgt cacgatgagt cacgacgcgt     60 

cacgcatatg tcacgacaaa tcacgacaga tacagataca gataaagaat taaaccccac    120 

acataacgcg cgcatgcgcg agagtgctac gaccggtgag ttgaatgacg cgtcgttgca    180 

gacagccgaa cctgaatacc tggacggcct gagcgaaccg atcgggaaat ttccgatgac    240 

tggagtctgg caaccgtcgc cggattttc                                      269 
 
           
             319  
             269  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            319 

gaaaatccgg cgacggttgc cagactccag tcatcggaaa tttcccgatc ggttcgctca     60 

ggccgtccag gtattcaggt tcggctgtct gcaacgacgc gtcattcaac tcaccggtcg    120 

tagcactctc gcgcatgcgc gcgttatgtg tggggtttaa ttctttatct gtatctgtat    180 

ctgtcgtgat ttgtcgtgac atatgcgtga cgcgtcgtga ctcatcgtga caatcagtgt    240 

tctgcttccg cagtctttcc cgctcccgc                                      269 

 
           
             320  
             269  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            320 

gcgggagcgg gaaagactgc ggaagcagaa cactgattgt cacgatgagt cacgacgcgt     60 

cacgcatatg tcacgacaaa tcacgacaga tacagataca gataaagaat taaaccccac    120 

acataacgcg cgcatgcgcg agagtgctac gaccggtgag ttgaatgacg cgtcgttgca    180 

gacagccgaa cctgaatacc tggacggcct gagcgaaccg atcgggaaat ttccgatgac    240 

tggagtctgg caaccgtcgc cggattttc                                      269 

 
           
             321  
             59  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            321 

cctggacggt gctaccctgg acggtgctac cctggacggt gctaccgtgg acggtgcta      59 
 
           
             322  
             59  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            322 

tagcaccgtc cacggtagca ccgtccaggg tagcaccgtc cagggtagca ccgtccagg      59 

 
           
             323  
             59  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            323 

cctggacggt gctaccctgg acggtgctac cctggacggt gctaccgtgg acggtgcta      59 

 
           
             324  
             59  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            324 

tagcaccgtc cacggtagca ccgtccaggg tagcaccgtc cagggtagca ccgtccagg      59 

 
           
             325  
             15  
             DNA  
             Escherichia coli K-12  
           
            325 

cctggacggt gctac                                                      15 

 
           
             326  
             15  
             DNA  
             Escherichia coli K-12  
           
            326 

gtagcaccgt ccagg                                                      15 

 
           
             327  
             23  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            327 

ctgactctga ctctgactct ggc                                             23 

 
           
             328  
             23  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            328 

gccagagtca gagtcagagt cag                                             23 

 
           
             329  
             24  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            329 

tctgactctg actctgactc tggc                                            24 

 
           
             330  
             24  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            330 

gccagagtca gagtcagagt caga                                            24 

 
           
             331  
             54  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            331 

aaggtgaagg tgaaggtgaa ggtgaaggtg aaggtgaagg tgaaggtgaa ggtg           54 

 
           
             332  
             54  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            332 

caccttcacc ttcaccttca ccttcacctt caccttcacc ttcaccttca cctt           54 

 
           
             333  
             54  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            333 

aaggtgaagg tgaaggtgaa ggtgaaggtg aaggtgaagg tgaaggtgaa ggtg           54 

 
           
             334  
             54  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            334 

caccttcacc ttcaccttca ccttcacctt caccttcacc ttcaccttca cctt           54 

 
           
             335  
             76  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            335 

gcacctcatt gttgtcggcg ctctctgtgt ggagcacctc attgttgtcg gcgctctctg     60 

tgtggagcac ctcatt                                                     76 

 
           
             336  
             76  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            336 

aatgaggtgc tccacacaga gagcgccgac aacaatgagg tgctccacac agagagcgcc     60 

gacaacaatg aggtgc                                                     76 

 
           
             337  
             76  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            337 

gcacctcatt gttgtcggcg ctctctgtgt ggagcacctc attgttgtcg gcgctctctg     60 

tgtggagcac ctcatt                                                     76 

 
           
             338  
             76  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            338 

aatgaggtgc tccacacaga gagcgccgac aacaatgagg tgctccacac agagagcgcc     60 

gacaacaatg aggtgc                                                     76 

 
           
             339  
             43  
             DNA  
             Escherichia coli K-12  
           
            339 

gcacctcatt gttgtcggcg ctctctgtgt ggagcacctc att                       43 

 
           
             340  
             43  
             DNA  
             Escherichia coli K-12  
           
            340 

aatgaggtgc tccacacaga gagcgccgac aacaatgagg tgc                       43 

 
           
             341  
             123  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            341 

ccagttccgg atgatggcgg tgatacgcct gttccgccag acgacggcgg tgatacgcca     60 

gttccggatg atggcggtga tacgcctgtt ccgccagacg acggcggtga tacgccagtt    120 

ccg                                                                  123 

 
           
             342  
             123  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            342 

cggaactggc gtatcaccgc cgtcgtctgg cggaacaggc gtatcaccgc catcatccgg     60 

aactggcgta tcaccgccgt cgtctggcgg aacaggcgta tcaccgccat catccggaac    120 

tgg                                                                  123 

 
           
             343  
             123  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            343 

ccagttccgg atgatggcgg tgatacgcct gttccgccag acgacggcgg tgatacgcca     60 

gttccggatg atggcggtga tacgcctgtt ccgccagacg acggcggtga tacgccagtt    120 

ccg                                                                  123 

 
           
             344  
             123  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            344 

cggaactggc gtatcaccgc cgtcgtctgg cggaacaggc gtatcaccgc catcatccgg     60 

aactggcgta tcaccgccgt cgtctggcgg aacaggcgta tcaccgccat catccggaac    120 

tgg                                                                  123 

 
           
             345  
             32  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            345 

agcagaacag cagaacagca gaacagcaga ac                                   32 

 
           
             346  
             32  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            346 

gttctgctgt tctgctgttc tgctgttctg ct                                   32 

 
           
             347  
             32  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            347 

agcagaacag cagaacagca gaacagcaga ac                                   32 

 
           
             348  
             32  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            348 

gttctgctgt tctgctgttc tgctgttctg ct                                   32 

 
           
             349  
             8  
             DNA  
             Escherichia coli K-12  
           
            349 

agcagaac                                                               8 

 
           
             350  
             8  
             DNA  
             Escherichia coli K-12  
           
            350 

gttctgct                                                               8 

 
           
             351  
             134  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            351 

actcgctggc aagaactctg ccgtctggca gcaccaggag tggtgtaatg accacgcgcc     60 

tgactcgctg gcaagaactc tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg    120 

cctgactcgc tggc                                                      134 

 
           
             352  
             134  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            352 

gccagcgagt caggcgcgtg gtcattacac cactcctggt gctgccagac ggcagagttc     60 

ttgccagcga gtcaggcgcg tggtcattac accactcctg gtgctgccag acggcagagt    120 

tcttgccagc gagt                                                      134 

 
           
             353  
             134  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            353 

actcgctggc aagaactctg ccgtctggca gcaccaggag tggtgtaatg accacgcgcc     60 

tgactcgctg gcaagaactc tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg    120 

cctgactcgc tggc                                                      134 

 
           
             354  
             134  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            354 

gccagcgagt caggcgcgtg gtcattacac cactcctggt gctgccagac ggcagagttc     60 

ttgccagcga gtcaggcgcg tggtcattac accactcctg gtgctgccag acggcagagt    120 

tcttgccagc gagt                                                      134 

 
           
             355  
             72  
             DNA  
             Escherichia coli K-12  
           
            355  
           
             356  
             72  
             DNA  
             Escherichia coli K-12  
           
            356 

gccagcgagt caggcgcgtg gtcattacac cactcctggt gctgccagac ggcagagttc     60 

ttgccagcga gt                                                         72 

 
           
             357  
             38  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            357 

gcgtcttatc cggcctacag cgtcttatcc ggcctaca                             38 

 
           
             358  
             38  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            358 

tgtaggccgg ataagacgct gtaggccgga taagacgc                             38 

 
           
             359  
             38  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            359 

gcgtcttatc cggcctacag cgtcttatcc ggcctaca                             38 

 
           
             360  
             38  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            360 

tgtaggccgg ataagacgct gtaggccgga taagacgc                             38 

 
           
             361  
             38  
             DNA  
             Escherichia coli K-12  
           
            361 

gcgtcttatc cggcctacag cgtcttatcc ggcctaca                             38 

 
           
             362  
             38  
             DNA  
             Escherichia coli K-12  
           
            362 

tgtaggccgg ataagacgct gtaggccgga taagacgc                             38 

 
           
             363  
             60  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            363 

aaatagaaat agaaatagaa atagaaatag aaatagaaat agaaatagaa atagaaatat     60 

 
           
             364  
             60  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            364 

aaatagaaat agaaatagaa atagaaatag aaatagaaat agaaatagaa atagaaatat     60 

 
           
             365  
             66  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            365 

aaatagaaat agaaatagaa atagaaatag aaatagaaat agaaatagaa atagaaatag     60 

aaatat                                                                66 

 
           
             366  
             66  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            366 

atatttctat ttctatttct atttctattt ctatttctat ttctatttct atttctattt     60 

ctattt                                                                66 

 
           
             367  
             154  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            367 

ggctctggc tctggctctg gctctggctc tggctctggc tctggctctg gctctggctc      60 

ggctctggc tctggctctg gctctggctc tggctctggc tctggctctg gctctggctc     120 

ggctctggc tctggctctg gctctggctc tggc                                 154 

 
           
             368  
             154  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            368 

ccagagcca gagccagagc cagagccaga gccagagcca gagccagagc cagagccaga      60 

ccagagcca gagccagagc cagagccaga gccagagcca gagccagagc cagagccaga     120 

ccagagcca gagccagagc cagagccaga gcca                                 154 

 
           
             369  
             106  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            369 

tggctctggc tctggctctg gctctggctc tggctctggc tctggctctg gctctggctc     60 

tggctctggc tctggctctg gctctggctc tggctctggc tctggc                    06 

 
           
             370  
             106  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            370 

gccagagcca gagccagagc cagagccaga gccagagcca gagccagagc cagagccaga     60 

gccagagcca gagccagagc cagagccaga gccagagcca gagcca                    06 

 
           
             371  
             33  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            371 

ggtgcaggtg caggtgcagg tgcaggtgca ggt                                  33 

 
           
             372  
             33  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            372 

acctgcacct gcacctgcac ctgcacctgc acc                                  33 

 
           
             373  
             33  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            373 

ggtgcaggtg caggtgcagg tgcaggtgca ggt                                  33 

 
           
             374  
             33  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            374 

acctgcacct gcacctgcac ctgcacctgc acc                                  33 

 
           
             375  
             15  
             DNA  
             Escherichia coli K-12  
           
            375 

ggtgcaggtg caggt                                                      15 

 
           
             376  
             15  
             DNA  
             Escherichia coli K-12  
           
            376 

acctgcacct gcacc                                                      15 

 
           
             377  
             58  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            377 

caaaatcagg aaccaggtcg aggtgctttc aaaatcagga accaggtcga ggtgcttt       58 

 
           
             378  
             58  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            378 

aaagcacctc gacctggttc ctgattttga aagcacctcg acctggttcc tgattttg       58 

 
           
             379  
             58  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            379 

caaaatcagg aaccaggtcg aggtgctttc aaaatcagga accaggtcga ggtgcttt       58 

 
           
             380  
             58  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            380 

aaagcacctc gacctggttc ctgattttga aagcacctcg acctggttcc tgattttg       58 

 
           
             381  
             36  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            381 

ccgccagcac cgccagcacc gccagcaccg ccagca                               36 

 
           
             382  
             36  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            382 

tgctggcggt gctggcggtg ctggcggtgc tggcgg                               36 

 
           
             383  
             36  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            383 

ccgccagcac cgccagcacc gccagcaccg ccagca                               36 

 
           
             384  
             36  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            384 

tgctggcggt gctggcggtg ctggcggtgc tggcgg                               36 

 
           
             385  
             18  
             DNA  
             Escherichia coli K-12  
           
            385 

ccgccagcac cgccagca                                                   18 

 
           
             386  
             18  
             DNA  
             Escherichia coli K-12  
           
            386 

tgctggcggt gctggcgg                                                   18 

 
           
             387  
             42  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            387 

catttgatct ttattgttat tcatttgatc tttattgtta tt                        42 

 
           
             388  
             42  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            388 

aataacaata aagatcaaat gaataacaat aaagatcaaa tg                        42 

 
           
             389  
             42  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            389 

catttgatct ttattgttat tcatttgatc tttattgtta tt                        42 

 
           
             390  
             42  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            390 

aataacaata aagatcaaat gaataacaat aaagatcaaa tg                        42 

 
           
             391  
             48  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            391 

tatctttatc tttatcttta tctttatctt tatctttatc tttatctt                  48 

 
           
             392  
             48  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            392 

aagataaaga taaagataaa gataaagata aagataaaga taaagata                  48 

 
           
             393  
             36  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            393 

tatctttatc tttatcttta tctttatctt tatctt                               36 

 
           
             394  
             36  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            394 

aagataaaga taaagataaa gataaagata aagata                               36 

 
           
             395  
             64  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            395 

agagcgaata atgacgattg cagagcgaat tcataatgac gattgcagag cgaattcata     60 

atga                                                                  64 

 
           
             396  
             64  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            396 

tcattatgaa ttcgctctgc aatcgtcatt atgaattcgc tctgcaatcg tcattattcg     60 

ctct                                                                  64 

 
           
             397  
             64  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            397 

agagcgaata atgacgattg cagagcgaat tcataatgac gattgcagag cgaattcata     60 

atga                                                                  64 

 
           
             398  
             64  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            398 

tcattatgaa ttcgctctgc aatcgtcatt atgaattcgc tctgcaatcg tcattattcg     60 

ctct                                                                  64 

 
           
             399  
             24  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            399 

gaccacgacc acgaccacga ccac                                            24 

 
           
             400  
             24  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            400 

gtggtcgtgg tcgtggtcgt ggtc                                            24 

 
           
             401  
             36  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            401 

gaccacgacc acgaccacga ccacgaccac gaccac                               36 

 
           
             402  
             36  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            402 

gtggtcgtgg tcgtggtcgt ggtcgtggtc gtggtc                               36 

 
           
             403  
             6  
             DNA  
             Escherichia coli K-12  
           
            403 

caccac                                                                 6 

 
           
             404  
             6  
             DNA  
             Escherichia coli K-12  
           
            404 

gtggtg                                                                 6 

 
           
             405  
             24  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            405 

tgcaaatgca aatgcaaatg caaa                                            24 

 
           
             406  
             24  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            406 

tttgcatttg catttgcatt tgca                                            24 

 
           
             407  
             31  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            407 

tgcaaatgca aatgcaaatt gcaaatgcaa a                                    31 

 
           
             408  
             31  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            408 

tttgcatttg caatttgcat ttgcatttgc a                                    31 

 
           
             409  
             33  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            409 

ttgtagttgt agttgtagta gttgttgttg ttg                                  33 

 
           
             410  
             33  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            410 

caacaacaac aactactaca actacaacta caa                                  33 

 
           
             411  
             33  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            411 

ttgtagttgt agttgtagta gttgttgttg ttg                                  33 

 
           
             412  
             33  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            412 

caacaacaac aactactaca actacaacta caa                                  33 

 
           
             413  
             27  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            413 

tcaatgattt caatgatttc aatgatg                                         27 

 
           
             414  
             27  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            414 

catcattgaa atcattgaaa tcattga                                         27 

 
           
             415  
             27  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            415 

tcaatgattt caatgatttc aatgatg                                         27 

 
           
             416  
             27  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            416 

catcattgaa atcattgaaa tcattga                                         27 

 
           
             417  
             35  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            417 

ggcggcatgg gtggcatggg tggcatgggc ggcat                                35 

 
           
             418  
             35  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            418 

atgccgccca tgccacccat gccacccatg ccgcc                                35 

 
           
             419  
             35  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            419 

ggcggcatgg gtggcatggg tggcatgggc ggcat                                35 

 
           
             420  
             35  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            420 

atgccgccca tgccacccat gccacccatg ccgcc                                35 

 
           
             421  
             51  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            421 

aaagtgctat gcagtaaaaa gtgctatgca gtaaaaagtg ctatgcagta a              51 

 
           
             422  
             51  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            422 

ttactgcata gcacttttta ctgcatagca ctttttactg catagcactt t              51 

 
           
             423  
             51  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            423 

aaagtgctat gcagtaaaaa gtgctatgca gtaaaaagtg ctatgcagta a              51 

 
           
             424  
             51  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            424 

ttactgcata gcacttttta ctgcatagca ctttttactg catagcactt t              51 

 
           
             425  
             158  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            425 

agaagttaa ataatctaca gaagttaaat aatctacaga agttaaataa tctacagaag      60 

taaataata tacagaagtt aaataatata caggagttaa ataattcgca ggagttaaat     120 

attcgcagg agttaaataa ttcgcaggag ttaaataa                             158 

 
           
             426  
             158  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            426 

tatttaact cctgcgaatt atttaactcc tgcgaattat ttaactcctg cgaattattt      60 

actcctgta tattatttaa cttctgtata ttatttaact tctgtagatt atttaacttc     120 

gtagattat ttaacttctg tagattattt aacttctg                             158 

 
           
             427  
             176  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            427 

agaagttaa ataatctaca gaagttaaat aatctacaga agttaaataa tctacagaag      60 

taaataatc tacagaagtt aaataatata cagaagttaa ataatataca ggagttaaat     120 

attcgcagg agttaaataa ttcgcaggag ttaaataatt cgcaggagtt aaataa         176 

 
           
             428  
             176  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            428 

tatttaact cctgcgaatt atttaactcc tgcgaattat ttaactcctg cgaattattt      60 

actcctgta tattatttaa cttctgtata ttatttaact tctgtagatt atttaacttc     120 

gtagattat ttaacttctg tagattattt aacttctgta gattatttaa cttctg         176 

 
           
             429  
             70  
             DNA  
             Escherichia coli O157H7 pO157  
           
            429 

acctcacacc tcacacctca cacctcacac ctcacacctc acacctcaca cctcacacct     60 

cacacctcac                                                            70 

 
           
             430  
             18  
             DNA  
             Escherichia coli O157H7 pO157  
           
            430 

tgctggcggt gctggcgg                                                   18 

 
           
             431  
             42  
             DNA  
             Escherichia coli O157H7 pO157  
           
            431 

tgctactgct actgctactg ctactgctac tgctactgct ac                        42 

 
           
             432  
             42  
             DNA  
             Escherichia coli O157H7 pO157  
           
            432 

gtagcagtag cagtagcagt agcagtagca gtagcagtag ca                        42 

 
           
             433  
             12  
             DNA  
             Escherichia coli O157H7 pOSAK1  
           
            433 

tgatgatgat gg                                                         12 

 
           
             434  
             12  
             DNA  
             Escherichia coli O157H7 pOSAK1  
           
            434 

ccatcatcat ca                                                         12 

 
           
             435  
             20  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            435 

tgttatgtta tgttatgttg                                                 20 

 
           
             436  
             20  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            436 

caacataaca taacataaca                                                 20 

 
           
             437  
             20  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            437 

tgttatgtta tgttatgttg                                                 20 

 
           
             438  
             20  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            438 

caacataaca taacataaca                                                 20 

 
           
             439  
             14  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            439 

tgcaacttgc aact                                                       14 

 
           
             440  
             14  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            440 

agttgcaagt tgca                                                       14 

 
           
             441  
             14  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            441 

tgcaacttgc aact                                                       14 

 
           
             442  
             14  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            442 

agttgcaagt tgca                                                       14 

 
           
             443  
             56  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            443 

agcagaaaaa tccacgccag tatcagcaag cagaaaaatc cacgccagta tcagca         56 

 
           
             444  
             56  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            444 

tgctgatact ggcgtggatt tttctgcttg ctgatactgg cgtggatttt tctgct         56 

 
           
             445  
             56  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            445 

agcagaaaaa tccacgccag tatcagcaag cagaaaaatc cacgccagta tcagca         56 

 
           
             446  
             56  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            446 

tgctgatact ggcgtggatt tttctgcttg ctgatactgg cgtggatttt tctgct         56 

 
           
             447  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            447 

tgctgttttt gctgtttt                                                   18 

 
           
             448  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            448 

aaaacagcaa aaacagca                                                   18 

 
           
             449  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            449 

tgctgttttt gctgtttt                                                   18 

 
           
             450  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            450 

aaaacagcaa aaacagca                                                   18 

 
           
             451  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            451 

aacccggtga acccggtg                                                   18 

 
           
             452  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            452 

caccgggttc accgggtt                                                   18 

 
           
             453  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            453 

aacccggtga acccggtg                                                   18 

 
           
             454  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            454 

caccgggttc accgggtt                                                   18 

 
           
             455  
             16  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            455 

ataaaataaa ataaaa                                                     16 

 
           
             456  
             16  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            456 

ttttatttta ttttat                                                     16 

 
           
             457  
             16  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            457 

ataaaataaa ataaaa                                                     16 

 
           
             458  
             16  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            458 

ttttatttta ttttat                                                     16 

 
           
             459  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            459 

gagccggagc cggagctg                                                   18 

 
           
             460  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            460 

cagctccggc tccggctc                                                   18 

 
           
             461  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            461 

gagccggagc cggagctg                                                   18 

 
           
             462  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            462 

cagctccggc tccggctc                                                   18 

 
           
             463  
             19  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            463 

gattgccgga ttgccggat                                                  19 

 
           
             464  
             19  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            464 

atccggcaat ccggcaatc                                                  19 

 
           
             465  
             19  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            465 

gattgccgga ttgccggat                                                  19 

 
           
             466  
             19  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            466 

atccggcaat ccggcaatc                                                  19 

 
           
             467  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            467 

agcgccagcg ccagcgcc                                                   18 

 
           
             468  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            468 

ggcgctggcg ctggcgct                                                   18 

 
           
             469  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            469 

agcgccagcg ccagcgcc                                                   18 

 
           
             470  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            470 

ggcgctggcg ctggcgct                                                   18 

 
           
             471  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            471 

cgacttcgac ttcgactt                                                   18 

 
           
             472  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            472 

aagtcgaagt cgaagtcg                                                   18 

 
           
             473  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            473 

cgacttcgac ttcgactt                                                   18 

 
           
             474  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            474 

aagtcgaagt cgaagtcg                                                   18 

 
           
             475  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            475 

tatctgtatc tgtatctg                                                   18 

 
           
             476  
             18  
             DNA  
             Escherichia coli O157H7 Sakai  
           
            476 

cagatacaga tacagata                                                   18 

 
           
             477  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            477 

tatctgtatc tgtatctg                                                   18 

 
           
             478  
             18  
             DNA  
             Escherichia coli O157H7 EDL933  
           
            478 

tatctgtatc tgtatctg                                                   18 

 
           
             479  
             15  
             DNA  
             Escherichia coli O157H7  
           
            479 

cctggacggt gctac                                                      15 

 
           
             480  
             15  
             DNA  
             Escherichia coli O157H7  
           
            480 

gtagcaccgt ccagg                                                      15 

 
           
             481  
             11  
             DNA  
             Escherichia coli O157H7  
           
            481 

tctgactctg a                                                          11 

 
           
             482  
             11  
             DNA  
             Escherichia coli O157H7  
           
            482 

tcagagtcag a                                                          11 

 
           
             483  
             6  
             DNA  
             Escherichia coli O157H7  
           
            483 

aaggtg                                                                 6 

 
           
             484  
             6  
             DNA  
             Escherichia coli O157H7  
           
            484 

cacctt                                                                 6 

 
           
             485  
             33  
             DNA  
             Escherichia coli O157H7  
           
            485 

gcacctcatt gttgtcggcg ctctctgtgt gga                                  33 

 
           
             486  
             33  
             DNA  
             Escherichia coli O157H7  
           
            486 

tccacacaga gagcgccgac aacaatgagg tgc                                  33 

 
           
             487  
             57  
             DNA  
             Escherichia coli O157H7  
           
            487 

ccagttccgg atgatggcgg tgatacgcct gttccgccag acgacggcgg tgatacg        57 

 
           
             488  
             57  
             DNA  
             Escherichia coli O157H7  
           
            488 

cgtatcaccg ccgtcgtctg gcggaacagg cgtatcaccg ccatcatccg gaactgg        57 

 
           
             489  
             8  
             DNA  
             Escherichia coli O157H7  
           
            489 

agcagaac                                                               8 

 
           
             490  
             8  
             DNA  
             Escherichia coli O157H7  
           
            490 

gttctgct                                                               8 

 
           
             491  
             62  
             DNA  
             Escherichia coli O157H7  
           
            491 

actcgctggc aagaactctg ccgtctggca gcaccaggag tggtgtaatg accacgcgcc     60 

tg                                                                    62 

 
           
             492  
             62  
             DNA  
             Escherichia coli O157H7  
           
            492 

caggcgcgtg gtcattacac cactcctggt gctgccagac ggcagagttc ttgccagcga     60 

gt                                                                    62 

 
           
             493  
             19  
             DNA  
             Escherichia coli O157H7  
           
            493 

gcgtcttatc cggcctaca                                                  19 

 
           
             494  
             19  
             DNA  
             Escherichia coli O157H7  
           
            494 

tgtaggccgg ataagacgc                                                  19 

 
           
             495  
             6  
             DNA  
             Escherichia coli O157H7  
           
            495 

aaatag                                                                 6 

 
           
             496  
             6  
             DNA  
             Escherichia coli O157H7  
           
            496 

ctattt                                                                 6 

 
           
             497  
             6  
             DNA  
             Escherichia coli O157H7  
           
            497 

tggctc                                                                 6 

 
           
             498  
             6  
             DNA  
             Escherichia coli O157H7  
           
            498 

gagcca                                                                 6 

 
           
             499  
             6  
             DNA  
             Escherichia coli O157H7  
           
            499 

ggtgca                                                                 6 

 
           
             500  
             6  
             DNA  
             Escherichia coli O157H7  
           
            500 

tgcacc                                                                 6 

 
           
             501  
             29  
             DNA  
             Escherichia coli O157H7  
           
            501 

caaaatcagg aaccaggtcg aggtgcttt                                       29 

 
           
             502  
             29  
             DNA  
             Escherichia coli O157H7  
           
            502 

aaagcacctc gacctggttc ctgattttg                                       29 

 
           
             503  
             9  
             DNA  
             Escherichia coli O157H7  
           
            503 

ccgccagca                                                              9 

 
           
             504  
             9  
             DNA  
             Escherichia coli O157H7  
           
            504 

tgctggcgg                                                              9 

 
           
             505  
             21  
             DNA  
             Escherichia coli O157H7  
           
            505 

catttgatct ttattgttat t                                               21 

 
           
             506  
             21  
             DNA  
             Escherichia coli O157H7  
           
            506 

aataacaata aagatcaaat g                                               21 

 
           
             507  
             6  
             DNA  
             Escherichia coli O157H7  
           
            507 

tatctt                                                                 6 

 
           
             508  
             6  
             DNA  
             Escherichia coli O157H7  
           
            508 

aagata                                                                 6 

 
           
             509  
             21  
             DNA  
             Escherichia coli O157H7  
           
            509 

agagcgaata atgacgattg c                                               21 

 
           
             510  
             21  
             DNA  
             Escherichia coli O157H7  
           
            510 

gcaatcgtca ttattcgctc t                                               21 

 
           
             511  
             6  
             DNA  
             Escherichia coli O157H7  
           
            511 

gaccac                                                                 6 

 
           
             512  
             6  
             DNA  
             Escherichia coli O157H7  
           
            512 

gtggtc                                                                 6 

 
           
             513  
             6  
             DNA  
             Escherichia coli O157H7  
           
            513 

tgcaaa                                                                 6 

 
           
             514  
             6  
             DNA  
             Escherichia coli O157H7  
           
            514 

tttgca                                                                 6 

 
           
             515  
             6  
             DNA  
             Escherichia coli O157H7  
           
            515 

ttgtag                                                                 6 

 
           
             516  
             6  
             DNA  
             Escherichia coli O157H7  
           
            516 

ctacaa                                                                 6 

 
           
             517  
             3  
             DNA  
             Escherichia coli O157H7  
           
            517 

gtt                                                                    3 

 
           
             518  
             3  
             DNA  
             Escherichia coli O157H7  
           
            518 

aac                                                                    3 

 
           
             519  
             9  
             DNA  
             Escherichia coli O157H7  
           
            519 

tcaatgatt                                                              9 

 
           
             520  
             9  
             DNA  
             Escherichia coli O157H7  
           
            520 

aatcattga                                                              9 

 
           
             521  
             8  
             DNA  
             Escherichia coli O157H7  
           
            521 

ggcggcat                                                               8 

 
           
             522  
             8  
             DNA  
             Escherichia coli O157H7  
           
            522 

atgccgcc                                                               8 

 
           
             523  
             9  
             DNA  
             Escherichia coli O157H7  
           
            523 

gggtggcat                                                              9 

 
           
             524  
             9  
             DNA  
             Escherichia coli O157H7  
           
            524 

atgccaccc                                                              9 

 
           
             525  
             17  
             DNA  
             Escherichia coli O157H7  
           
            525 

aaagtgctat gcagtaa                                                    17 

 
           
             526  
             17  
             DNA  
             Escherichia coli O157H7  
           
            526 

ttactgcata gcacttt                                                    17 

 
           
             527  
             69  
             DNA  
             Escherichia coli O157H7  
           
            527 

cagaagttaa ataatctaca gaagttaaat aatctacaga agttaaataa tctacagaag     60 

ttaaataat                                                             69 

 
           
             528  
             69  
             DNA  
             Escherichia coli O157H7  
           
            528 

attatttaac ttctgtagat tatttaactt ctgtagatta tttaacttct gtagattatt     60 

taacttctg                                                             69 

 
           
             529  
             41  
             DNA  
             Escherichia coli O157H7  
           
            529 

tacagaagtt aaataatata cagaagttaa ataatataca g                         41 

 
           
             530  
             41  
             DNA  
             Escherichia coli O157H7  
           
            530 

ctgtatatta tttaacttct gtatattatt taacttctgt a                         41 

 
           
             531  
             68  
             DNA  
             Escherichia coli O157H7  
           
            531 

caggagttaa ataattcgca ggagttaaat aattcgcagg agttaaataa ttcgcaggag     60 

ttaaataa                                                              68 

 
           
             532  
             68  
             DNA  
             Escherichia coli O157H7  
           
            532 

ttatttaact cctgcgaatt atttaactcc tgcgaattat ttaactcctg cgaattattt     60 

aactcctg                                                              68 

 
           
             533  
             7  
             DNA  
             Escherichia coli O157H7  
           
            533 

acctcac                                                                7 

 
           
             534  
             7  
             DNA  
             Escherichia coli O157H7  
           
            534 

gtgaggt                                                                7 

 
           
             535  
             6  
             DNA  
             Escherichia coli O157H7  
           
            535 

tgctac                                                                 6 

 
           
             536  
             6  
             DNA  
             Escherichia coli O157H7  
           
            536 

gtagca                                                                 6 

 
           
             537  
             3  
             DNA  
             Escherichia coli O157H7  
           
            537 

tga                                                                    3 

 
           
             538  
             3  
             DNA  
             Escherichia coli O157H7  
           
            538 

tca                                                                    3 

 
           
             539  
             5  
             DNA  
             Escherichia coli O157H7  
           
            539 

tgtta                                                                  5 

 
           
             540  
             5  
             DNA  
             Escherichia coli O157H7  
           
            540 

taaca                                                                  5 

 
           
             541  
             7  
             DNA  
             Escherichia coli O157H7  
           
            541 

tgcaact                                                                7 

 
           
             542  
             7  
             DNA  
             Escherichia coli O157H7  
           
            542 

agttgca                                                                7 

 
           
             543  
             28  
             DNA  
             Escherichia coli O157H7  
           
            543 

agcagaaaaa tccacgccag tatcagca                                        28 

 
           
             544  
             28  
             DNA  
             Escherichia coli O157H7  
           
            544 

tgctgatact ggcgtggatt tttctgct                                        28 

 
           
             545  
             9  
             DNA  
             Escherichia coli O157H7  
           
            545 

tgctgtttt                                                              9 

 
           
             546  
             9  
             DNA  
             Escherichia coli O157H7  
           
            546 

aaaacagca                                                              9 

 
           
             547  
             9  
             DNA  
             Escherichia coli O157H7  
           
            547 

aacccggtg                                                              9 

 
           
             548  
             9  
             DNA  
             Escherichia coli O157H7  
           
            548 

caccgggtt                                                              9 

 
           
             549  
             5  
             DNA  
             Escherichia coli O157H7  
           
            549 

ataaa                                                                  5 

 
           
             550  
             5  
             DNA  
             Escherichia coli O157H7  
           
            550 

tttat                                                                  5 

 
           
             551  
             6  
             DNA  
             Escherichia coli O157H7  
           
            551 

gagccg                                                                 6 

 
           
             552  
             6  
             DNA  
             Escherichia coli O157H7  
           
            552 

cggctc                                                                 6 

 
           
             553  
             8  
             DNA  
             Escherichia coli O157H7  
           
            553 

gattgccg                                                               8 

 
           
             554  
             8  
             DNA  
             Escherichia coli O157H7  
           
            554 

cggcaatc                                                               8 

 
           
             555  
             6  
             DNA  
             Escherichia coli O157H7  
           
            555 

agcgcc                                                                 6 

 
           
             556  
             6  
             DNA  
             Escherichia coli O157H7  
           
            556 

ggcgct                                                                 6 

 
           
             557  
             6  
             DNA  
             Escherichia coli O157H7  
           
            557 

cgactt                                                                 6 

 
           
             558  
             6  
             DNA  
             Escherichia coli O157H7  
           
            558 

aagtcg                                                                 6 

 
           
             559  
             6  
             DNA  
             Escherichia coli O157H7  
           
            559 

tatctg                                                                 6 

 
           
             560  
             6  
             DNA  
             Escherichia coli O157H7  
           
            560 

cagata                                                                 6