Patent Publication Number: US-8975473-B2

Title: Stress tolerance and delayed senescence in plants

Description:
RELATED APPLICATION 
     This application is a Continuation of U.S. Ser. No. 11/229,371, filed Sep. 16, 2005, which in turn is a Continuation of U.S. Ser. No. 10/229,541, filed Aug. 27, 2002, which in turn is a Continuation-in-Part of U.S. Ser. No. 10/160,764, filed May 31, 2002, which claims the benefit of U.S. Ser. No. 60/294,766, filed May 31, 2001 and U.S. Ser. No. 60/348,909, filed Oct. 22, 2001; this application is also a Continuation-in-Part of U.S. Ser. No. 10/210,760, filed Aug. 1, 2002, which claims the benefit of U.S. Ser. No. 60/309,396, filed Aug. 1, 2001 and U.S. Ser. No. 60/337,084, filed Dec. 4, 2001; this application is also a Continuation-in-Part of U.S. Ser. No. 09/191,687, filed Nov. 13, 1998, which in turn is a Continuation-in-Part of PCT Application No. PCT/US98/15664, filed Jul. 29, 1998, and a Continuation-in-Part of U.S. Ser. No. 09/124,867, filed Jul. 30, 1998, both of which claim the benefit of U.S. Provisional Application No. 60/054,474, filed Aug. 1, 1997. The contents of each of these applications are incorporated herein by reference in their entireties. 
    
    
     BACKGROUND OF THE INVENTION 
     Most higher plants encounter at least transient decreases in relative water content at some stage of their life cycle and, as a result, have evolved a number of desiccation protection mechanisms. If however, the change in water deficit is prolonged the effects on the plant&#39;s growth and development can be profound. Decreased water content due to drought, cold or salt stresses can irreparably damage plant cells which in turn limits plant growth and crop productivity in agriculture. 
     Plants respond to adverse conditions of drought, salinity and cold with a variety of morphological and physiological changes. Although our understanding of plant tolerance mechanisms to these stresses is fragmentary, the plant hormone abscisic acid (ABA) has been proposed to be an essential mediator between environmental stimulus and plant responses. ABA levels increase in response to water deficits and exogenously applied ABA mimics many of the responses normally induced by water stress. Once ABA is synthesized it causes the closure of the leaf stomata thereby decreasing water loss through transpiration. 
     The identification of genes that transduce ABA into a cellular response opens the possibility of exploiting these regulators to enhance desiccation tolerance in crop species. In principle, these ABA signaling genes can be coupled with the appropriate controlling elements to allow optimal plant growth and development. Thus, not only would these genes allow the genetic tailoring of crops to withstand transitory environmental insults, they should also broaden the environments where traditional crops can be grown. 
     In addition, little is known of the genetic mechanisms which control plant growth and development. Genes which further affect other metabolic processes such as senescence and growth habits of plants can be useful in a wide variety of crop and horticultural plants. 
     SUMMARY OF THE INVENTION 
     This invention relates to isolated nucleic acids which encode a farnesyl transferase comprising SEQ ID NO:1 or SEQ ID NO:172. Nucleic acids also encompassed by this invention are such hybridizing sequences which encode the functional equivalent or fragment thereof of SEQ ID NO:1 or SEQ ID NO:172. The present invention also relates to a method for enhancing the drought tolerance of plants using inhibitors of the products encoded by these nucleic acids. Further, this invention relates to the control of regulatory functions in photosynthetic organisms; for example, in the control of growth habit, flowering, seed production, seed germination, and senescence in such organisms. 
     This invention also relates to a method for enhancing the drought or stress tolerance of plants by means of alterations in isolated or recombinant nucleic acids encoding a farnesyl transferase (Ftase) protein or fragment thereof or its functional equivalent. Nucleic acids which hybridize to the Ftase-encoding gene (ERA1) are also encompassed by this invention when such hybridizing sequences encode the functional equivalent of the Ftase protein. The present invention also relates to a method for enhancing the drought tolerance of plants through the genetic manipulation of ERA1 gene and its functional equivalents to improve stress tolerance in crop plants. Loss of ERA1 gene function confers enhanced tolerance to drought at the level of the mature plant. The nature of an era1 mutant with loss of Ftase activity, for example, demonstrates that inhibition of farnesylation enhances ABA responses in a plant. 
     Further, this invention relates to inhibition of senescence in photosynthetic organisms through inhibition of farnesyl transferase activity. The resulting photosynthetic organisms stay green and tissue viability is maintained for a longer period of time. Thus, methods to provide greener plants and a reduction in senescence are part of this invention. 
     In yet another embodiment, methods are provided to modify the growth habit and flower induction of plants. Loss of ERA1 gene function under particular environmental conditions results in a reduction in the number of lateral branches produced on a plant and an increase in the number of flowers per inflorescence. 
     The invention also provides method of producing a transgenic plant, which has an altered phenotype such as increased tolerance to stress (e.g., water deficit, increased biomass, increased yield), delayed senescence or increased ABA sensitivity by introducing into a plant cell a compound that inhibits farnesylation of a polypeptide comprising a CaaX motif. By inhibit farnesylation is meant to include that the compound inhibits one or more steps in the three step process of farnesylation. In one aspect the compound inhibits farnesyltransferase, prenylprotease or prenylcysteine carboxyl methyltransferase expression or activity. Alternatively, the compound is an anti-sense farnesyl transferase nucleic acid or a farnesyl transferase double stranded RNA-inhibition hair pin nucleic acid. In some aspects the nucleic acid is operably linked to a promoter such as a constitutive promoter, an ABA inducible promoter, tissue specific promoters or a guard cell-specific promoter. 
     Exemplary anti-sense nucleic acids are 20 or more consecutive nucleic acids complementary to SEQ ID NO: 1, 14, 40, 43, 80-85 or 172. Alternatively the anti-sense nucleic acids include SEQ ID NO: 36, 41, 44 or 54-64. 
     In various aspects the compound is a nucleic acid encoding a farnesyltransferase, prenylprotease or prenylcysteine carboxyl methyltransferase polypeptide of fragment thereof. Alternatively, the compound is a nucleic acid encoding a mutated farnesyltransferase, prenylprotease or prenylcysteine carboxyl methyltransferase polypeptide of fragment thereof. By mutated is meant that the polypeptide lacks at least one activity of the wild type polypeptide such as for example, subunit interaction, substrate binding or enzyme catalysis. A mutated polypeptide forms a dimer, such as a heterodimer. For example, a mutated farnesyltransferase beta polypeptide forms a dimer with a farnesyltransferase alpha polypeptide. Preferably, the polypeptide is less than 400, 350, 314, 300 or 200 amino acids in length. For example, the compound includes SEQ ID NO: 1, 14, 40, 43, 80-85 or 172. 
     In a further aspect the compound is a nucleic acid encoding a CaaX motif or a nucleic acid encoding a CaaX motif operably linked to a promoter. 
     Also included in the invention are the plants produced by the methods of the invention and the seed produced by the plants which produce a plant that has an altered phenotype. 
     This invention also relates to a regulatory sequence useful for genetic engineering of plant cells to provide a method of controlling the tissue pattern of expression of DNA sequences linked to this novel regulatory sequence. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIGS. 1A-1C  show the nucleic acid sequence of the ERA1 gene (SEQ ID NO: 1) in which the introns are underlined and the start codon (ATG) is at nucleotide positions 1-3. 
         FIG. 2  is the amino acid sequence of the ERA1 protein (SEQ ID NO:2). 
         FIGS. 3A-3B  show the nucleic acid sequence of the ERA1 promoter (SEQ ID NO:3). 
         FIG. 4  is the amino acid sequence of the β subunit farnesylation domain from  Arabidopsis  (Arab.) (SEQ ID NO:2) aligned with the β subunit farnesylation domains from pea (SEQ ID NO:4), yeast (SEQ ID NO:5) and rat (SEQ ID NO:6). Residues that are identical to the  Arabidopsis  sequence are indicated with a dot. A dash indicates a blank. The amino acid positions of the  Arabidopsis  gene are indicated on the right-hand side. 
         FIG. 5  is a photograph of an era1-transformed  Arabidopsis  plant (right) compared to the wild-type (control; i.e., naturally-occurring) plant (left) under extremely dry conditions. 
         FIG. 6  is a graph comparing the water content of  Arabidopsis  plants with inactivated or mutant Ftase activity (M. Columbia, era1-2) and controls (M.C. control, era1-2 control). 
         FIG. 7  is a graph comparing the rate of water loss for the  Arabidopsis  plants with inactivated or mutant Ftase activity (M. Columbia, era1-2) and controls (M.C. control, era1-2 control). 
         FIGS. 8A-8E  are comparisons of aging leaves from control (wild-type) and era-2 mutant plants. 
         FIGS. 9A-9C  are comparisons of transcript levels in aging leaves from control (wild-type) and era-2 mutant plants. 
         FIG. 10  is an illustration depicting the pBI121 antisense FTA vector construct. 
         FIG. 11  is an illustration of genomic Southern hybridization analysis of anti-FTA transgenic  Arabidopsis thaliana.    
         FIG. 12  is an illustration of Northern analysis of five 35S-anti-FTA  Arabidopsis thaliana  lines (T3 plants). 
         FIG. 13  shows a Western expression analysis using anti-FTA antibodies to detect the FTA polypeptides. 
         FIG. 14  is a set of photographs showing ABA effects on seedling growth and development. FTA antisense transgenic seedlings exhibit enhanced ABA sensitivity. 
         FIG. 15  shows the effect of ABA on seedling growth and development. 
         FIG. 16  shows photographs of wild type Columbia (A) and four antisense FTA transgenic lines (B, C, D, E) of  Arabidopsis thaliana  after 8 days without watering. 
         FIG. 17  is an illustration of the homology among FTA nucleic acid (A) and amino acid (B) sequences from various plant species based on ClustalW analysis (percent identity shown). 
         FIG. 18  is an illustration of the homology among FTB nucleic acid and amino acid sequences from various plant species based on ClustalW analysis (percent identity shown). 
         FIG. 19  is an illustration of transgenic performance during water stress. 
         FIG. 20  is an illustration of shoot fresh weight, or biomass accumulation, after 6 days of water stress treatment and 6 days recovery time. 
         FIG. 21  is an illustration of seed yield (grams) obtained under optimal conditions or following a 6 day water stress treatment. 
         FIG. 22  is an illustration of vegetative growth under optimal conditions, shown is shoot fresh weight 6 days after the first flower opened. 
         FIG. 23  is an illustration of the effect of a biotic stress coupled with drought stress treatment on seed yield. 
         FIG. 24  is a representative illustration of gel electrophoresis analysis of PCR products in an assay to detect transgenic lines of  Brassica napus.    
         FIG. 25  is a schematic representation of the vector constructs; A) pBI121-AtCPP, B) pBI121-antisense-AtCPP, C) pBI121-HP-AtCPP. 
         FIG. 26  is an illustration of (A) nucleic acid and (B) amino acid sequence identities as determined by ClustalW analysis. 
         FIG. 27  is a scan of a typical Southern blot of transgenic  Arabidopsis  T1 lines carrying the pBI121-AtCPP construct. 
         FIG. 28  is a scan of a typical Southern blot of transgenic  Arabidopsis  T3 lines carrying the pBI121-HP-AtCPP construct. 
         FIG. 29  is a scan of a typical Southern blot of transgenic  Arabidopsis  lines carrying the pRD29A-AtCPP construct. 
         FIG. 30  is a scan of a typical Southern blot of transgenic  Arabidopsis  lines carrying the pRD29A-HP-AtCPP construct. 
         FIG. 31  is an illustration showing the relative expression of AtCPP mRNA transcript (solid bars) and AtCPP protein levels (stippled bars) in several pBI121-AtCPP transgenic lines. 
         FIG. 32  is a histogram showing the percentage of lines which were categorized as ABA sensitive, moderately ABA sensitive or ABA insensitive. Seedlings were assessed on agar plates containing 1 μM ABA and scored at 21 days growth. Thirty-six lines of the pBI121-AtCPP over-expression construct were assessed at 21 days by leaf and seedling development. Thirty-two lines of the 35S-HP-AtCPP down-regulation construct were assessed at 21 days for leaf and seedling development. Each line was assessed by plating approximately 100 seeds per plate and the seedlings scored and recorded as the percent insensitive seedlings per plate. Each line was then expressed as a percent of wild type (Wt). Lines were categorized as sensitive (less than 1% of Wt) solid bars, intermediate (1-50% of Wt) diagonally lined or insensitive (greater than 50% of Wt) stippled, based on their relationship to Wt and the percentage of each category plotted as a histogram. 
         FIG. 33  is an illustration showing the response of wild type and a pRD29A-HP-AtCPP transgenic line to various concentrations of ABA in two week old seedlings. 
         FIG. 34  is a histogram showing the analysis of transgenic plants containing the pBI121-AtCPP over-expression construct (SEQ ID NO:4). Water loss per gram shoot dry weight after four days of water stress treatment. Lines that are marked with a star are those which were strongly ABA sensitive. Lines marked with a triangle are moderately ABA sensitive. Bars represent means of eight replicates. Lines marked with a filled dot above the bar represents lines which were significantly different from control at a p=0.05 value. 
         FIG. 35  is a histogram showing seed yield in grams of transgenic  Arabidopsis  lines of pBI121-AtCPP grown under optimal water conditions, 
         FIG. 36  is a bar chart showing growth and yield of transgenic  Arabidopsis  lines of pBI121-AtCPP grown under optimal watering conditions plus a biotic stress condition. Yields as % of wild type, rosette leaf number, rosette leaf fresh weight and shoot dry weight are plotted. 
         FIG. 37  are photographs showing growth of transgenic  Arabidopsis  lines of pBI121-AtCPP grown on agar plates. Changes to root growth are visible. 
         FIG. 38  is a bar chart showing growth of transgenic  Arabidopsis  lines of pRD29A-HP-AtCPP grown under optimal watering conditions. Rosette leaf number, rosette leaf dry weight and shoot dry weight are plotted. 
         FIG. 39  is a photograph showing Northern blot of ΔN90AtFTB  Arabidopsis  plants, 
     
    
    
     The foregoing and other objects, features and advantages of the invention will be apparent from the following more particular description of preferred embodiments of the invention, as illustrated in the accompanying drawings in which like reference characters refer to the same parts throughout the different views. The drawings are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the invention. 
     DETAILED DESCRIPTION OF THE INVENTION 
     This invention relates to transgenic plants that display an altered phenotype, e.g., increased tolerance to stress, delayed senescence, increased ABA sensitivity, increased yield, increased productivity and increased biomass and methods of producing the plants by introducing to a plant cell a compound that inhibits farnesylation of a polypeptide comprising a CaaX motif 
     Protein farnesylation, the addition of a C-terminal, 15 carbon chain to protein and subsequent processing is a three step enzymatic reaction including farnesylation, proteolytic cleavage and methylation. First, a farnesyltransferase adds the C-terminal 15 carbon chain to a conserved cysteine residue of the CaaX terminal motif, where “C” is a cysteine, “a” is an aliphatic amino acid and “X” is any amino acid. Second, the last three amino acid residues (aaX) are cleaved by a prenyl protease. Lastly, the modified cysteine is methylated by a methylase to create the final active product of the protein farnesylation pathway. The Applicants have shown that over-expression and down-regulation of the alpha or the beta farnesyl transferase gene in plant cells (i.e., the first step in farnesylation) results in plants with an altered phenotype such as but not limited to drought tolerance and delayed senescence. Applicants have also shown that over-expression and down-regulation of the prenyl protease gene (i.e., the second step in farnesylation) in plant cells also results in a plant displaying an altered phenotype including for example but not limited to drought tolerance and increased resistance to biotic and abiotic stress. These results taken together support the hypothesis that modification of the expression of any of the enzymes in the farnesylation pathway (farnesyltransferase, prenylprotease or prenylcysteine carboxyl methyltransferase in a plant cell will result in a plant displaying an altered phenotype. 
     The present invention also provides novel farnesyltransferase (i.e., alpha and beta) (Ftase) and CaaX prenyl protease (CPP) nucleic acid sequences isolated from for example  Arabidopsis thaliana  (At),  Brassica napus  (Bn) and  Glycine Max  (Gm). The invention also provides farnesyltransferase and CaaX prenyl protease antisense nucleic acids and constructs comprising these nucleic acids. The sequences are collectively referred to as “PPI nucleic acids”, “PPI polynucleotides” or “PPI antisense nucleic acids” and the corresponding encoded polypeptide is referred to as a “PPI polypeptide” or “PPI protein”. Unless indicated otherwise, “PPI” is meant to refer to any of the novel sequences disclosed herein. Table A below summarizes the nucleic acids and polypeptides according to the invention. 
     
       
         
           
               
               
               
             
               
                 TABLE A 
               
               
                   
               
               
                   
                   
                 SEQ ID 
               
               
                   
                 PPI Sequence Description 
                 NO: 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 era1 (FTB) 
                 1 
               
               
                   
                 era1 (FTB) 
                 2 
               
               
                   
                 ERa1 promoter 
                 3 
               
               
                   
                 FTB pea 
                 4 
               
               
                   
                 FTB yeast 
                 5 
               
               
                   
                 FTB rat 
                 6 
               
               
                   
                 At FTA 
                 7 
               
               
                   
                 At FTA 
                 8 
               
               
                   
                 At FTA 
                 9 
               
               
                   
                 pBI121-35S-anti-AtFTA 
                 10 
               
               
                   
                 At FTA 
                 11 
               
               
                   
                 Bn FTA 
                 12 
               
               
                   
                 Bn FTA 
                 13 
               
               
                   
                 Bn FTB 
                 14 
               
               
                   
                 Bn FTB 
                 15 
               
               
                   
                 primer 
                 16 
               
               
                   
                 primer 
                 17 
               
               
                   
                 primer 
                 18 
               
               
                   
                 primer 
                 19 
               
               
                   
                 primer 
                 20 
               
               
                   
                 primer 
                 21 
               
               
                   
                 primer 
                 22 
               
               
                   
                 primer 
                 23 
               
               
                   
                 primer 
                 24 
               
               
                   
                 primer 
                 25 
               
               
                   
                 primer 
                 26 
               
               
                   
                 primer 
                 27 
               
               
                   
                 primer 
                 28 
               
               
                   
                 primer 
                 29 
               
               
                   
                 primer 
                 30 
               
               
                   
                 primer 
                 31 
               
               
                   
                 primer 
                 32 
               
               
                   
                 primer 
                 33 
               
               
                   
                 primer 
                 34 
               
               
                   
                 Bn FTA 
                 35 
               
               
                   
                 Bn FTB 
                 36 
               
               
                   
                 G max FTA 
                 37 
               
               
                   
                 G max FTA 
                 38 
               
               
                   
                 G max FTA 
                 39 
               
               
                   
                 G max FTB 
                 40 
               
               
                   
                 G max FTB 
                 41 
               
               
                   
                 G max FTB 
                 42 
               
               
                   
                 Zea maize FTB 
                 43 
               
               
                   
                 Zea maize FTB 
                 44 
               
               
                   
                 Zea maize FTB 
                 45 
               
               
                   
                 pBI121-35S-AtFTA 
                 46 
               
               
                   
                 pBI121-rd29A-anti-AtFTA 
                 47 
               
               
                   
                 pBI121-35S-DA-AtFTA 
                 48 
               
               
                   
                 pBI121-RD29A-DA-AtFTA 
                 49 
               
               
                   
                 MuA-anti-GmFTA 
                 50 
               
               
                   
                 RD29A-anti-GmFTA 
                 51 
               
               
                   
                 MuA-HP-GmFTA-Nos-Term 
                 52 
               
               
                   
                 RD29AP-HP-GmFTA-Nos-Term 
                 53 
               
               
                   
                 pBI121-35S-Anti-AtFTB 
                 54 
               
               
                   
                 pBI121-RD29AP-Anti-AtFTB 
                 55 
               
               
                   
                 pBI121-35S-HP-AtFTB 
                 56 
               
               
                   
                 pBI121-RD29AP-HP-AtFTB 
                 57 
               
               
                   
                 pBI121-35S-AtFTB 
                 58 
               
               
                   
                 MuA-anti-GmFTB-Nos-Term 
                 59 
               
               
                   
                 RD29AP-anti-GmFTB-Nos-Term 
                 60 
               
               
                   
                 MuA-HP-GmFTB-Nos-Term 
                 61 
               
               
                   
                 RD29AP-HP-GmFTB-Nos-Term 
                 62 
               
               
                   
                 MuA-anti-Zea maizeFTB-Nos- 
                 63 
               
               
                   
                 Term 
                   
               
               
                   
                 MuA-HP-Zea maizeFTB-Nos- 
                 64 
               
               
                   
                 Term 
                   
               
               
                   
                 Pea-FT-A 
                 65 
               
               
                   
                 Tomato-FTA 
                 66 
               
               
                   
                 Rice-FT-A 
                 67 
               
               
                   
                 Zea mays-FT-A 
                 68 
               
               
                   
                 Soy1-Ft-A 
                 69 
               
               
                   
                 Soy2-FT-A 
                 70 
               
               
                   
                 Triticum-FT-A 
                 71 
               
               
                   
                 Pea-FT-A 
                 72 
               
               
                   
                 Tomato-FTA 
                 73 
               
               
                   
                 Rice-FT-A 
                 74 
               
               
                   
                 Zea mays-FT-A 
                 75 
               
               
                   
                 Soy1-Ft-A 
                 76 
               
               
                   
                 Soy2-FT-A 
                 77 
               
               
                   
                 Triticum-FT-A 
                 78 
               
               
                   
                 N90AtFTB truncated FTB vector 
                 79 
               
               
                   
                 Wiggum (FTB) 
                 80 
               
               
                   
                 Dup-Soy-FTB 
                 81 
               
               
                   
                 Dup-Corn-FTB 
                 82 
               
               
                   
                 Pea-FT-B 
                 83 
               
               
                   
                 Tomato-FTB 
                 84 
               
               
                   
                 Tobacco-FTB 
                 85 
               
               
                   
                 Primer SacI forward 
                 86 
               
               
                   
                 Wiggum (FTB) 
                 87 
               
               
                   
                 Dup-Soy-FTB 
                 88 
               
               
                   
                 Dup-Corn-FTB 
                 89 
               
               
                   
                 Pea-FT-B 
                 90 
               
               
                   
                 Tomato-FTB 
                 91 
               
               
                   
                 Tobacco-FTB 
                 92 
               
               
                   
                 Consensus FTA 
                 93 
               
               
                   
                 Consensus FTB 
                 94 
               
               
                   
                 Consensus FTA 
                 95 
               
               
                   
                 Consensus FTB 
                 96 
               
               
                   
                 AtCPP 
                 97 
               
               
                   
                 AtCPP 
                 98 
               
               
                   
                 At-AFC1 
                   
               
               
                   
                 pBI121-AtCPP 
                 99 
               
               
                   
                 pBI121-HP-AtCPP 
                 100 
               
               
                   
                 AtCPP BamFW 
                 101 
               
               
                   
                 AtCPP SmaRV 
                 102 
               
               
                   
                 AtCPP-HP-SacFW 
                 103 
               
               
                   
                 AtCPP-HP-SacRV 
                 104 
               
               
                   
                 pBI121-AtCPP Forward 
                 105 
               
               
                   
                 pBI121-antiAtCPP-SmaFW 
                 106 
               
               
                   
                 pBI121-antiAtCPP-BamRV 
                 107 
               
               
                   
                 p35S-HP-AtCPP Reverse 
                 108 
               
               
                   
                 BnCPP 
                 109 
               
               
                   
                 BnCPP 
                 110 
               
               
                   
                 BnCPP antisense 
                 111 
               
               
                   
                 GmCPP 
                 112 
               
               
                   
                 GmCPP 
                 113 
               
               
                   
                 GmCPP antisense 
                 114 
               
               
                   
                 AtCPP antisense 
                 115 
               
               
                   
                 BASF-AT1 
                 116 
               
               
                   
                 BASF-AT1 
                 117 
               
               
                   
                 BASF-AT2 
                 118 
               
               
                   
                 BASF-AT2 
                 119 
               
               
                   
                 BASF-Corn 
                 120 
               
               
                   
                 BASF-Corn 
                 121 
               
               
                   
                 BASF-Soy 
                 122 
               
               
                   
                 BASF-Soy 
                 123 
               
               
                   
                 AFC1 
                 124 
               
               
                   
                 AFC1 
                 125 
               
               
                   
                 AT4g01320 
                 126 
               
               
                   
                 AT4g01320 
                 127 
               
               
                   
                 AF007269 
                 128 
               
               
                   
                 AF007269 
                 129 
               
               
                   
                 pBI121-antisense-AtCPP 
                 130 
               
               
                   
                 pRD29A-AtCPP 
                 131 
               
               
                   
                 pRD29A-HP-AtCPP 
                 132 
               
               
                   
                 pRD29A-antisense-AtCPP 
                 133 
               
               
                   
                 MuA-AtCPP 
                 134 
               
               
                   
                 MuA-GmCPP 
                 135 
               
               
                   
                 pBI121-GmCPP 
                 136 
               
               
                   
                 pBI121-HP-GmCPP 
                 137 
               
               
                   
                 pBI121-antisense-GmCPP 
                 138 
               
               
                   
                 pRD29A-GmCPP 
                 139 
               
               
                   
                 pRD29A-HP-GmCPP 
                 140 
               
               
                   
                 pRD29A-antisense-GmCPP 
                 141 
               
               
                   
                 pBI121-BnCPP 
                 142 
               
               
                   
                 pBI121-HP-BnCPP 
                 143 
               
               
                   
                 pBI121-antisense-BnCPP 
                 144 
               
               
                   
                 pRD29A-BnCPP 
                 145 
               
               
                   
                 pRD29A-HP-BnCPP 
                 146 
               
               
                   
                 pRD29A-antisense-BnCPP 
                 147 
               
               
                   
                 MuA-BnCPP 
                 148 
               
               
                   
                 GmCPP SmaFW 
                 149 
               
               
                   
                 GmCPP SacRV 
                 150 
               
               
                   
                 BnCPP-anti-SmaFW 
                 151 
               
               
                   
                 BnCPP-anti-BamRV 
                 152 
               
               
                   
                 BnCPP-HP-Sac-FW 
                 153 
               
               
                   
                 BnCPP-HP-Sac-RV 
                 154 
               
               
                   
                 BnCPP-HP-BamFW 
                 155 
               
               
                   
                 BnCPP-HP-XbaRV 
                 156 
               
               
                   
                 GmCPP-HP-Sac-FW 
                 157 
               
               
                   
                 GmCPP-HP-Sac-RV 
                 158 
               
               
                   
                 GmCPP-HP-BamFW 
                 159 
               
               
                   
                 GmCPP-HP-XbaRV 
                 160 
               
               
                   
                 pRD29AP 
                 161 
               
               
                   
                 Nosterm-RV 
                 162 
               
               
                   
                 Consensus-BASF 
                 163 
               
               
                   
                 Consensus-BASF 
                 164 
               
               
                   
                 Consensus-Generic 
                 165 
               
               
                   
                 Consensus-Generic 
                 166 
               
               
                   
                 Consensus-PPI 
                 167 
               
               
                   
                 Consensus-PPI 
                 168 
               
               
                   
                 Consensus-PPI/Generic 
                 169 
               
               
                   
                 Consensus-PPI/Generic 
                 170 
               
               
                   
                 Primer BamHI REV 
                 171 
               
               
                   
                 Full Length AtFTB 
                 172 
               
               
                   
                 pBI121-AtFTB full length 
                 173 
               
               
                   
                 primer 
                 174 
               
               
                   
                 primer 
                 175 
               
               
                   
                 isoprenylcysteine carboxyl 
                 176 
               
               
                   
                 methyltransferase 
                   
               
               
                   
                 Full Length AtFTB 
                 177 
               
               
                   
               
            
           
         
       
     
     This invention also relates to isolated nucleic acids and proteins encoded by these nucleic acids which modify the growth, reproduction and senescence of plants. In particular, the constructs of this invention include an isolated nucleic acid encoding a farnesyl transferase (Ftase) polypeptide comprising SEQ ID NO: 1 or 172 or its functional equivalent or fragment thereof, and the Ftase polypeptides or proteins of fragments thereof encoded by these nucleic acids. In particular, this invention relates to a protein wherein the sequence is SEQ ID NO:2 or SEQ ID NO:177. 
     Further included in this invention are nucleic acid constructs which comprise a promoter (ERA1 promoter) operably-linked to isolated nucleic acid comprising SEQ ID NO: 1 or 172 or its functional equivalent or a complement of either. When incorporated into a plant, the ERA1 promoter is regulated in the guard cells of the plant and can affect water loss through the stomates. This promoter consists of a nucleic acid comprising SEQ ID NO:3 ( FIG. 3 ). 
     Transgenic plants, seeds, plant cell and tissues incorporating these constructs are also part of this invention. Accordingly, in one aspect of this invention, a method is provided for producing a gene product under the control of a promoter which operates primarily in guard cells through expression of a gene encoding the gene product in the cell of a plant comprising the steps of: transforming a plant cell with a DNA construct comprising a) a regulatory region comprising SEQ ID NO:3 or a functional portion thereof, DNA comprising a structural gene encoding a gene product, and a 3′ untranslated region containing a polyadenylated region; regenerating a plant, photosynthetic organism or tissue culture from the cell; and placing the plant, photosynthetic organisms or tissue culture under conditions so that the promoter induces transcription of the structural gene and the gene product is expressed. 
     In the context of this disclosure, the terms “regulatory region” or “promoter” refer to a sequence of DNA, usually upstream (5′) to the coding sequence of a structural gene, which controls the expression of the coding region by providing recognition and binding sites for RNA polymerase and/or other factors required for transcription to start at the correct site. The term “functional portion” or “functional fragment” refers to a truncated sequence of a promoter of this invention which maintains the capability of inducing transcription of an ERA structural gene under the conditions described for activity of an Ftase protein. 
     The constructs and methods described herein can be applied to all types of plants and other photosynthetic organisms, including, but not limited to: angiosperms (monocots and dicots), gymnosperms, spore-bearing or vegetatively-reproducing plants and the algae, including the cyanophyta (blue-green algae). Particularly preferred plants are those plants which provide commercially-valuable crops, such as corn, wheat, cotton, rice, canola, sugar cane, sugar beet, sunflowers, potatoes, tomatoes, broccoli, carrots, lettuce, apple, plum, orange, lemon, rose, and the like. 
     Further, the constructs and methods of this invention can be adapted to any plant part, protoplast, or tissue culture wherein the tissue is derived from a photosynthetic organism. The term “plant part” is meant to include a portion of a plant capable of producing a regenerated plant. Preferable plant parts include roots and shoots and meristematic portions thereof. Other plant parts encompassed by this invention are: leaves, flowers, seeds, epicotyls, hypocotyls, cotyledons, cotyledonary nodes, explants, pollen, ovules, meristematic or embryonic tissue, protoplasts, and the like. Transgenic plants can be regenerated from any of these plant parts, including tissue culture or protoplasts, and also from explants. Methods will vary according to the species of plant. 
     This invention relates to compositions and constructs comprising isolated nucleic acids (both DNA and RNA) encoding an Ftase and portions thereof of photosynthetic organisms. This invention further relates to compositions and constructs comprising isolated nucleic acids encoding an Ftase promoter. In particular, the ERA1 gene encoding the β subunit of Ftase from  Arabidopsis  and a regulatory sequence which regulates the transcription of the ERA1 gene have been isolated and sequenced. Nucleic acids which encode Ftases from photosynthetic organisms, and homologues or analogs of these nucleic acids, are encompassed by this invention. 
     The invention further relates to methods using isolated and/or recombinant nucleic acids (DNA or RNA) that are characterized by their ability to hybridize to (a) a nucleic acid encoding an Ftase protein or polypeptide, such as a nucleic acid having the sequences of SEQ ID NO: 1 or 172 or (b) a portion of the foregoing (e.g., a portion comprising the minimum nucleotides required to encode a functional Ftase protein; or by the ability to encode a polypeptide having the amino acid sequence of an Ftase (e.g., SEQ ID NO:2 or SEQ ID NO: 177, or to encode functional equivalents thereof, e.g., a polypeptide having at least 80% sequence similarity to SEQ ID NO:2 or SEQ ID NO:177, which when incorporated into a plant cell, facilitates the growth habit, seed germination, and metabolism in a photosynthetic organism in the same manner as SEQ ID NO: 1 or 172). A functional equivalent of an Ftase therefore, would have at least an 80% similar amino acid sequence and similar characteristics to, or perform in substantially the same way as, the polypeptide encoded by SEQ ID NO:2 or SEQ ID NO:177. A nucleic acid which hybridizes to a nucleic acid encoding an Ftase polypeptide such as SEQ ID NO:2 or SEQ ID NO: 177 can be double- or single-stranded. Hybridization to DNA such as DNA having the sequence SEQ ID NO: 1 or 172, includes hybridization to the strand shown or its complementary strand. 
     In one embodiment, the percent amino acid sequence similarity between an Ftase polypeptide such as SEQ ID NO:2 or SEQ ID NO: 177, and functional equivalents thereof is at least about 60% (≧60%). In a preferred embodiment, the percent amino acid sequence similarity between an Ftase polypeptide and its functional equivalents is at least about 75% (≧75%). More preferably, the percent amino acid sequence similarity between an Ftase polypeptide and its functional equivalents is at least about 80%, and still more preferably, at least about 90%, when consecutive amino acids are compared. 
     Isolated and/or recombinant nucleic acids meeting these criteria comprise nucleic acids having sequences identical to sequences of naturally occurring ERA1 genes and portions thereof, or variants of the naturally occurring genes. Such variants include mutants differing by the addition, deletion or substitution of one or more nucleotides, modified nucleic acids in which one or more nucleotides are modified (e.g., DNA or RNA analogs), and mutants comprising one or more modified nucleotides. 
     Such nucleic acids, including DNA or RNA, can be detected and isolated by hybridization under high stringency conditions or moderate stringency conditions, for example, which are chosen so as to not permit the hybridization of nucleic acids having non-complementary sequences. “Stringency conditions” for hybridizations is a term of art which refers to the conditions of temperature and buffer concentration which permit hybridization of a particular nucleic acid to another nucleic acid in which the first nucleic acid may be perfectly complementary to the second, or the first and second may share some degree of complementarity which is less than perfect. For example, certain high stringency conditions can be used which distinguish perfectly complementary nucleic acids from those of less complementarity. “High stringency conditions” and “moderate stringency conditions” for nucleic acid hybridizations are explained on pages 2.10.1-2.10.16 (see particularly 2.10.8-11) and pages 6.3.1-6 in  Current Protocols in Molecular Biology  (Ausubel, F. M. et al., eds., Vol. 1, containing supplements up through Supplement 29, 1995), the teachings of which are hereby incorporated by reference. The exact conditions which determine the stringency of hybridization depend not only on ionic strength, temperature and the concentration of destabilizing agents such as formamide, but also on factors such as the length of the nucleic acid sequence, base composition, percent mismatch between hybridizing sequences and the frequency of occurrence of subsets of that sequence within other non-identical sequences. Thus, high or moderate stringency conditions can be determined empirically. 
     High stringency hybridization procedures can (1) employ low ionic strength and high temperature for washing, such as 0.015 M NaCl/0.0015 M sodium citrate, pH 7.0 (0.1×SSC) with 0.1% sodium dodecyl sulfate (SDS) at 50° C.; (2) employ during hybridization 50% (vol/vol) formamide with 5×Denhardt&#39;s solution (0.1% weight/volume highly purified bovine serum albumin/0.1% wt/vol Ficoll/0.1% wt/vol polyvinylpyrrolidone), 50 mM sodium phosphate buffer at pH 6.5 and 5×SSC at 42° C.; or (3) employ hybridization with 50% formamide, 5×SSC, 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5×Denhardt&#39;s solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC and 0.1% SDS. Moderate stringency conditions would be similar except that hybridization would employ 25% formamide in place of 50% formamide. 
     By varying hybridization conditions from a level of stringency at which no hybridization occurs to a level at which hybridization is first observed, conditions which will allow a given sequence to hybridize with the most similar sequences in the sample can be determined. 
     Exemplary conditions are described in Krause, M. H. and S. A. Aaronson (1991)  Methods in Enzymology,  200:546-556. Also, see especially page 2.10.11 in  Current Protocols in Molecular Biology  (supra), which describes how to determine washing conditions for moderate or low stringency conditions. Washing is the step in which conditions are usually set so as to determine a minimum level of complementarity of the hybrids. Generally, from the lowest temperature at which only homologous hybridization occurs, a 1% mismatch between hybridizing nucleic acids results in a 1° C. decrease in the melting temperature T m , for any chosen SSC concentration. Generally, doubling the concentration of SSC results in an increase in T m  of ≈17° C. Using these guidelines, the washing temperature can be determined empirically for moderate or low stringency, depending on the level of mismatch sought. 
     Isolated and/or recombinant nucleic acids that are characterized by their ability to hybridize to (a) a nucleic acid encoding an Ftase polypeptide, such as the nucleic acids depicted as SEQ ID NO:1 or 172, (b) the complement of SEQ ID NO: 1 or 172, (c) or a portion of (a) or (b) (e.g. under high or moderate stringency conditions), may further encode a protein or polypeptide having at least one functional characteristic of an Ftase polypeptide, such as regulation of lateral branching under diurnal light cycles, or regulation of the response to ABA, or regulation of senescence. 
     Enzymatic assays, complementation tests, or other suitable methods can also be used in procedures for the identification and/or isolation of nucleic acids which encode a polypeptide such as a polypeptide of the amino acid sequence SEQ ID NO:2 or SEQ ID NO:177 or a functional equivalent or fragment thereof of this polypeptide. The antigenic properties of proteins or polypeptides encoded by hybridizing nucleic acids can be determined by immunological methods employing antibodies that bind to an Ftase polypeptide such as immunoblot, immunoprecipitation and radioimmunoassay. PCR methodology, including RAGE (Rapid Amplification of Genomic DNA Ends), can also be used to screen for and detect the presence of nucleic acids which encode Ftase-like proteins and polypeptides, and to assist in cloning such nucleic acids from genomic DNA. PCR methods for these purposes can be found in Innis, M. A., et al (1990)  PCR Protocols: A Guide to Methods and Applications , Academic Press, Inc., San Diego, Calif., incorporated herein by reference. 
     The nucleic acids described herein are used in the methods of the present invention for production of proteins or polypeptides which are incorporated into cells, tissues, plant parts, plants and other photosynthetic organisms. In one embodiment, DNA containing all or part of the coding sequence for an Ftase polypeptide, or DNA which hybridizes to DNA having the sequence SEQ ID NO:2 or SEQ ID NO: 177 is incorporated into a vector for expression of the encoded polypeptide in suitable host cells. The encoded polypeptide consisting of an Ftase subunit or its functional equivalent is capable of farnesyl transferase activity. The term “vector” as used herein refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. 
     Primers and probes consisting of 20 or more contiguous nucleotides of the above-described nucleic acids are also included as part of this invention. Thus, one nucleic acid of this invention comprises a specific sequence of about 20 to about 200 or more nucleotides which are identical or complementary to a specific sequence of nucleotides of the Ftase protein-encoding DNA or transcribed mRNA. These probes and primers can be used to identify and isolate Ftase-encoding nucleic acid from other photosynthetic organisms. 
     Nucleic acids referred to herein as “isolated” are nucleic acids separated away from the nucleic acids of the genomic DNA or cellular RNA of their source of origin (e.g., as it exists in cells or in a mixture of nucleic acids such as a library), and may have undergone further processing. “Isolated” nucleic acids include nucleic acids obtained by methods described herein, similar methods or other suitable methods, including essentially pure nucleic acids, nucleic acids produced by chemical synthesis, by combinations of biological and chemical methods, and recombinant nucleic acids which are isolated. Nucleic acids referred to herein as “recombinant” are nucleic acids which have been produced by recombinant DNA methodology, including those nucleic acids that are generated by procedures which rely upon a method of artificial recombination, such as the polymerase chain reaction (PCR) and/or cloning into a vector using restriction enzymes. “Recombinant” nucleic acids are also those that result from recombination events that occur through the natural mechanisms of cells, but are selected for after the introduction to the cells of nucleic acids designed to allow or make probable a desired recombination event. Portions of the isolated nucleic acids which code for polypeptides having a certain function can be identified and isolated by, for example, the method of Jasin, M., et al., U.S. Pat. No. 4,952,501. 
     A further embodiment of the invention is antisense nucleic acids or oligonucleotides which are complementary, in whole or in part, to a target molecule comprising a sense strand, and can hybridize with the target molecule. The target can be DNA, or its RNA counterpart (i.e., wherein T residues of the DNA are U residues in the RNA counterpart). When introduced into a cell, antisense nucleic acids or oligonucleotides can inhibit the expression of the gene encoded by the sense strand or the mRNA transcribed from the sense strand. Antisense nucleic acids can be produced by standard techniques. See, for example, Shewmaker, et al., U.S. Pat. No. 5,107,065. 
     In a particular embodiment, an antisense nucleic acid or oligonucleotide is wholly or partially complementary to and can hybridize with a target nucleic acid (either DNA or RNA), wherein the target nucleic acid can hybridize to a nucleic acid having the sequence of the complement of the strand in SEQ ID NO: 1 or 172. For example, an antisense nucleic acid or oligonucleotide can be complementary to a target nucleic acid having the sequence shown as the strand of the open reading frame of SEQ ID NO: 1 or 172, or nucleic acid encoding a functional equivalent or fragment thereof of Ftase, or to a portion of these nucleic acids sufficient to allow hybridization. A portion, for example, a sequence of 16 nucleotides could be sufficient to inhibit expression of the protein. Fragments comprising 25 or more consecutive nucleotides complementary to SEQ ID NO: 1 or 172 could also be used. Or, an antisense nucleic acid or oligonucleotide complementary to 5′ or 3′ untranslated regions, or overlapping the translation initiation codon (5′ untranslated and translated regions), of the ERA1 gene, or a gene encoding a functional equivalent or fragment thereof can also be effective. In another embodiment, the antisense nucleic acid is wholly or partially complementary to and can hybridize with a target nucleic acid which encodes an Ftase polypeptide. 
     In addition to the antisense nucleic acids of the invention, oligonucleotides can be constructed which will bind to duplex nucleic acid either in the gene or the DNA:RNA complex of transcription, to form a stable triple helix-containing or triplex nucleic acid to inhibit transcription and/or expression of a gene encoding an Ftase polypeptide or its functional equivalent. Frank-Kamenetskii, M. D. and Mirkin, S. M. (1995)  Ann. Rev. Biochem.  64:65-95. Such oligonucleotides of the invention are constructed using the base-pairing rules of triple helix formation and the nucleotide sequence of the gene or mRNA for Ftase. These oligonucleotides can block Ftase-type activity in a number of ways, including prevention of transcription of the ERA1 gene or by binding to mRNA as it is transcribed by the gene. 
     Another aspect of the invention pertains to the use of post transcriptional gene silencing (PTGS) to repress gene expression. Double stranded RNA can initiate the sequence specific repression of gene expression in plants and animals. Double stranded RNA is processed to short duplex oligomers of 21-23 nucleotides in length. These small interfering RNA&#39;s suppress the expression of endogenous and heterologous genes in a sequence specific manner (Fire et al. Nature 391:806-811, Carthew, Curr. Opin. in Cell Biol., 13:244-248, Elbashir et al., Nature 411:494-498). A RNAi suppressing construct can be designed in a number of ways, for example, transcription of a inverted repeat which can form a long hair pin molecule, inverted repeats separated by a spacer sequence that could be an unrelated sequence such as GUS or an intron sequence. Transcription of sense and antisense strands by opposing promoters or cotranscription of sense and antisense genes. 
     Another aspect of the invention pertains to the use of post transcriptional gene silencing (PTGS) to repress gene expression. Double stranded RNA can initiate the sequence specific repression of gene expression in plants and animals. Double stranded RNA is processed to short duplex oligomers of 21-23 nucleotides in length. These small interfering RNA&#39;s suppress the expression of endogenous and heterologous genes in a sequence specific manner (Fire et al. Nature 391:806-811, Carthew, Curr. Opin. in Cell Biol., 13:244-248, Elbashir et al., Nature 411:494-498). A RNAi suppressing construct can be designed in a number of ways, for example, transcription of a inverted repeat which can form a long hair pin molecule, inverted repeats separated by a spacer sequence that could be an unrelated sequence such as GUS or an intron sequence. Transcription of sense and antisense strands by opposing promoters or cotranscription of sense and antisense genes. 
     Another aspect of the invention pertains to the use of the dominant-negative genetic approach. Briefly the presence of a dominant trait, i.e. the expression of a transgene, results in a reduction of enzyme activity or reduced production of the enzymatic end-product. It has been demonstrated that FT is a heterodimer formed by α- and β-subunits. FT activity relies on the proper dimerization between these subunits to form functional enzyme. Expression of a non-functional subunit will interact with the second subunit to produce a non-functional enzyme and hence reduced enzymatic activity. The non-functional aspect may be in respect to, but not limited to, subunit interaction, substrate binding or enzyme catalysis, for example. Alternatively the expressed trait may produce a substrate analogue which competes with native substrate, the end result being decreased farnesylation of biologically active substrate. 
     The invention also relates to proteins or polypeptides encoded by the novel nucleic acids described herein. The proteins and polypeptides of this invention can be isolated and/or recombinant. Proteins or polypeptides referred to herein as “isolated” are proteins or polypeptides purified to a state beyond that in which they exist in cells. In a preferred embodiment, they are at least 10% pure; i.e., substantially purified. “Isolated” proteins or polypeptides include proteins or polypeptides obtained by methods described infra, similar methods or other suitable methods, and include essentially pure proteins or polypeptides, proteins or polypeptides produced by chemical synthesis or by combinations of biological and chemical methods, and recombinant proteins or polypeptides which are isolated. Proteins or polypeptides referred to herein as “recombinant” are proteins or polypeptides produced by the expression of recombinant nucleic acids. 
     In a preferred embodiment, the protein or portion thereof has at least one function characteristic of an Ftase; for example, catalytic activity affecting, e.g., normal lateral branching, florets/inflorescence, seed germination, or stomatal opening, and binding function, and/or antigenic function (e.g., binding of antibodies that also bind to naturally occurring Ftase). As such, these proteins are referred to as Ftases of plant origin, and include, for example, naturally occurring Ftase, variants (e.g. mutants) of those proteins and/or portions thereof. Such variants include mutants differing by the addition, deletion or substitution of one or more amino acid residues, or modified polypeptides in which one or more residues are modified, and mutants comprising one or more modified residues. 
     The invention also relates to isolated and/or recombinant portions of an Ftase as described above, especially the β subunit of an Ftase protein. Portions of the enzyme can be made which have full or partial function on their own, or which when mixed together (though fully, partially, or nonfunctional alone), spontaneously assemble with one or more other polypeptides to reconstitute a functional protein having at least one functional characteristic of an Ftase of this invention. 
     A number of genes have been identified that are induced by ABA. This suggests that ABA-induced tolerance to adverse environmental conditions is a complex multigenic event. Thus, identification and transfer of single genes into crop plants which improves the viability of the plant under different environmental conditions due to increased responsiveness to ABA is novel and extremely useful. 
     To identify genes that could be more global controllers of ABA-regulated plant processes, genetic screens were applied in a number of plant species to isolate mutations that alter the response of the plant to the hormone. 
     Mutations that confer enhanced response to ABA (era) in  Arabidopsis  seeds were identified by their ability to prevent seed germination with low concentrations of ABA that normally permit wild-type (controls, i.e., naturally-occurring) seed germination. Of these, the era1 mutant class, which includes one transferred DNA (T-DNA) line (era1-1, ecotype Wassilewskija) and two neutron-generated mutants (era1-2 and era1-3, ecotype Columbia), was of added interest because this class showed decreased germination efficiency under normal postimbibition. Mutations that enhance ABA responsiveness should, in principle, be more dormant. Dormancy in era1 alleles was alleviated by a 4-day chilling period; the efficiency of era1 germination increased with the length of time the seeds are chilled. In many plant species, breaking dormancy to allow germination requires vernalization and exposure to moist, low-temperature environments for an extended period (Baskin and Baskin, 1971). The germination profile of era mutants could reflect an increased state of ABA-induced dormancy; consequently, these seeds require longer vernalization to germinate. Support for this contention came from construction of double mutants of era1 with both ABA biosynthetic (aba1-1) and insensitive mutants (abi1-1 and abi3-6). In all cases, the double mutants had reduced dormancy as compared with era1, indicating that the increased dormancy observed in era1 seed was dependent on ABA synthesis or sensitivity. 
     Aside from broadening the spectrum of new ABA response mutants, supersensitivity screens were also used to identify negative regulators of ABA sensitivity. That is, inhibition of these gene functions enhances the ABA response. One of these genes (ERA1) has been cloned and demonstrated to encode the β-subunit of a heterodimeric protein farnesyl transferase (Ftase) (Cutler et al., 1996). The era1-1 mutation, which is due to a T-DNA insertion, allowed the isolation of plant genomic regions flanking the insertions. Using the flanking regions as probes, the wild-type cDNA and genomic clones were isolated. Sequence analysis of these described a gene encompassing 3.5 kb of genomic DNA. The gene contains 13 introns which are underlined in  FIGS. 1A-1C  and the T-DNA insertion site in era1-1 is in intron 8. Southern (DNA) analysis of wild-type DNA, era1-2, and era1-3 probed with Era1cDNA revealed that both fast-neutron alleles contain deletions spanning the ERA4 locus. Fast-neutron mutagenesis induced small deletions in  Arabidopsis  (Shirley et al., 1992), and subsequent genomic analysis with a 14-kb probe that spans the ERA1 locus determined the size of the era1-2 deletion to be about 7.5 kb and the era1-3 deletion to be slightly larger. Thus all three era1 alleles contained DNA disruptions at the same locus, confirming the identity of the ERA locus. 
     Conceptual translation of the longest open reading frame ( 404  amino acids) in the ERA1 gene produced a protein ( FIGS. 2 and 4 ) with a high sequence similarity to yeast, pea, and mammalian protein farnesyl transferase β subunit genes (Goodman et al., 1988; Chen et al., 1991; Yang et al., 1993). Farnesyl transferases consist of α and β subunits that dimerize, forming an enzyme that catalyzes the attachment of farnesyl pyrophosphate (15 carbons) to proteins containing a COOH-terminal CaaX motif (Schafer and Rine, 1992), where C designates cysteine residue, aa is usually aliphatic amino acids, and X may designate a cysteine, serine, methionine, or glutamine residue. Both plant β subunit genes contain a region of about 50 amino acids near their COOH-terminus that is absent in yeast and animal β subunit genes. 
     In yeast and mammalian systems, Ftases modify several signal transduction proteins for membrane localization. This is achieved by the attachment of the lipophilic farnesyl sidechain to the protein target via the Ftase. The attachment of the farnesyl group causes a change in the overall hydrophobicity of the target allowing the protein to anchor itself into the membrane where it usually interacts with other signal transduction molecules. That the loss of farnesylation activity in the era1 mutant leads to an enhanced response of the seed to ABA suggests a target protein in  Arabidopsis  must be localized to the membrane to attenuate the ABA signal. Thus farnesylation in  Arabidopsis , appears to be required for the normal function of a negative regulator of ABA sensitivity. 
     Subsequent work has shown that loss of ERA1 gene function in  Arabidopsis  confers an enhanced tolerance to environmental stresses at the level of the mature plant. For example, a comparison of wild-type plants and era1 mutant plants grown in soil under standard laboratory conditions (24 hr light, 150 μE m- 2  sec- 1 , 30% humidity) showed that the mutants did not require water as frequently as the wild-type plants in order to maintain viability ( FIG. 5 ). When mutant and wild-type plants were grown until flowering occurred, watering was stopped and the plants were observed each subsequent day for signs of stress. Water loss was significantly reduced in the mutant plants compared to the wild-type plants ( FIGS. 6 and 7 ). 
     To determine if the observed increased drought tolerance of era mutants was related to ERA1 gene function, transgenic plants containing a ERA1 promoter fusion to a reporter GUS gene (made by inserting a 5 Kb fragment of the ERA1 promoter into a promoterless GUS T-DNA plasmid), were constructed. Analysis of the transgenic plants showed that ERA1 is transcriptionally expressed in the epidermal tissue of  Arabidopsis  and that this expression is guard-cell specific. Expression of ERA1 was also noted in the meristematic tissue of the plants and in root hairs. The guard cell expression of ERA1 is consistent with the drought tolerance of the mutant as these cells are the major regulators of water transpiration through the plant. It would be expected that ERA1-regulated stomatal conductance would require expression of the ERA1 gene in the guard cells. Hence loss of ERA1 gene function results in guard cells which are more responsive to ABA which, in turn, leads to more drought responsive guard cell regulation. Therefore, modification of Ftase expression or activity in higher plants, especially crop plants, will have profound effects on stomatal conductance and transpiration rates in the plants. 
     The nature of the era1 mutation in  Arabidopsis  demonstrates that inhibition of farnesylation will enhance ABA responses in a plant and alteration of this enzyme activity in crop species. Inhibition of Ftase activity in crop plants can be achieved via a number of methods. For example, antisense technology of cognate ERA1 genes in a variety of crop species can be used to reduce Ftase activity, thus increasing drought tolerance. By specifically producing ERA1 antisense RNA in guard cells, the amount of Ftase synthesized can be reduced to a level which would mimic era mutant phenotypes. The ERA1 promoter is regulated in a number of different tissues ranging from shoot meristems to root hairs. By determining the elements of the ERA1 promoter which allow expression in specific tissues, it is possible to tailor the expression of antisense ERA1 to only one tissue or cell type, such as guard cells. 
     Another method to inhibit Ftase activity in plants is the production of specific peptide inhibitors of farnesylation in transgenic plants. In mammalian and yeast systems, the carboxyl terminal target sequence (CaaX, where C=cysteine, x=aliphatic, X=any amino acid) which allows the attachment of the farnesyl group to specific proteins has been clearly defined. Peptides which mimic these target sequences have been made and shown to inhibit farnesylation of the endogenous target proteins in these systems. Moreover, CAIM is farnesylated in vivo in  Arabidopsis . Thus, similar inhibitors can be applied to higher plants to competitively inhibit Ftase in vivo. Again, this can be done through expression of inhibitor peptides in transgenic plants by synthesizing the DNA sequence for a CaaX peptide and fusing it to a guard cell-specific promoter. In both methods, using the appropriate promoters, antisense Ftase or peptide inhibitors can be specifically targeted and controlled. 
     Also included in the invention are methods of producing a transgenic plant. The method includes introducing into one or more plant cells a compound that alters, e.g., inhibits farnesylation of a polypeptide having a carboxyl terminal CaaX motif in the plant to generate a transgenic plant cell and regenerating a transgenic plant from the transgenic cell. In some aspects the compound alters, e.g., increases or decreases CaaX prenyl protease expression or activity. Alternatively, the compound alters farnesyltransferase expression or activity. In other aspects the compound alters isoprenylcysteine carboxyl methyltransferase expression or activity. The compound can be, e.g., (i) a CaaX prenyl protease, farnesyltransferase or isoprenylcysteine carboxyl methyltransferase polypeptide; (ii) a nucleic acid encoding a CaaX prenyl protease, farnesyltransferase or isoprenylcysteine carboxyl methyltransferase polypeptide; (iii) a nucleic acid that increases expression of a nucleic acid that encodes a CaaX prenyl protease, farnesyltransferase or isoprenylcysteine carboxyl methyltransferase polypeptide; (iv) a nucleic acid that decreases the expression of a nucleic acid that encodes a CaaX prenyl protease, farnesyltransferase or isoprenylcysteine carboxyl methyltransferase polypeptide; (v) a CaaX prenyl protease, farnesyltransferase or isoprenylcysteine carboxyl methyltransferase antisense nucleic acid and derivatives, fragments, analogs and homologs thereof. A nucleic acid that increases expression of a nucleic acid that encodes a CaaX prenyl protease, farnesyltransferase or isoprenylcysteine carboxyl methyltransferase polypeptide includes, e.g., promoters, enhancers. The nucleic acid can be either endogenous or exogenous. Preferably, the compound is a CaaX prenyl protease, farnesyltransferase or isoprenylcysteine carboxyl methyltransferase polypeptide or a nucleic acid encoding a CaaX prenyl protease, farnesyltransferase or isoprenylcysteine carboxyl methyltransferase polypeptide. 
     Included in the invention are methods of producing a transgenic plant that has increased stress resistance, delayed senescence or increased sensitivity to ABA. The method includes introducing into one or more plant cells a compound that alters farnesyl transferase expression (i.e., farnesyl transferase alpha or beta) or activity in the plant. The compound can be, e.g., (i) a farnesyl transferase polypeptide inhibitor; (ii) a nucleic acid encoding a farnesyl transferase polypeptide inhibitor; (iii) a nucleic acid that decreases expression of a nucleic acid that encodes a farnesyl transferase polypeptide and, derivatives, fragments, analogs and homologs thereof; (iv) an antisense farnesyl transferase nucleic acid. A nucleic acid that decreases expression of a nucleic acid that encodes a farnesyl transferase polypeptide includes, e.g., antisense nucleic acids or RNA inhibitory nucleic acids. The nucleic acid can be either endogenous or exogenous. Preferably the compound is a farnesyl transferase polypeptide or a nucleic acid encoding a farnesyl transferase polypeptide. More preferably the compound is a nucleic acid complementary to a nucleic acid encoding a farnesyl transferase polypeptide. For example, an anti-sense nucleic acid molecule. 
     Alternatively the compound is a nucleic acid molecule comprising a nucleic acid sequence encoding a mutated farnesyl transferase, isoprenylcysteine carboxyl methyltransferase or CaaX prenyl protease polypeptide. By mutated is meant that the polypeptide lacks one or more function of a wild-type polypeptide. For example, a mutated farnesyltransferase beta polypeptide is a polypeptide has less amino acids than a full length wild type polypeptide by still retains the ability to dimerize with an alpha subunit. For example a mutated farnesyltransferase beta polypeptide is less than 314 amino acids in length. Preferably, the mutated farnesyltransferase beta polypeptide comprises the amino acid sequence of SEQ ID NO:1 or a fragment thereof. 
     In another aspect the compound is a nucleic acid encoding a CaaX motif. Alternatively, the CaaX motif is operably liked to a promoter. 
     Also included in the invention is a plant where a mutation has been introduced in the gene encoding farnesyl transferase (i.e., alpha or beta) which results in a plant that has decreased farnesyl transferase activity and increased tolerance to stress as compared to a wild type plant. The mutation may be introduced by chemical or mechanical means. 
     In various aspects the transgenic plant has an altered phenotype as compared to a wild type plant (i.e., untransformed). By altered phenotype is meant that the plant has a one or more characteristic that is different from the wild type plant. For example, the transgenic plant has an increased resistance to stress. Increased stress resistance is meant that the transgenic plant can grow under stress conditions (e.g., high salt, decreased water, low temperatures, high temperatures) or under conditions that normally inhibit the growth of an untransformed, Stresses include, for example, chilling stress, heat stress, heat shock, salt stress, water stress (i.e., drought), nutritional stress, disease, grazing pests, wound healing, pathogens such as for example fungi, bacteria, nematodes, viruses or parasitic weed and herbicides. Methodologies to determine plant growth or response to stress include for example, height measurements, weight or biomass measurements, leaf area or number, ability to flower, water use, transpiration rates and yield. Alternatively, the transformed plant has an increased (i.e., enhanced) ABA sensitivity. The enhanced ABA sensitivity is at the seedling growth stage. Alternatively, the enhanced ABA sensitivity is at the mature plant stage. Additional altered phenotypes include for example, enhanced vegetative growth (e.g., increased leaf number, thickness and overall biomass), delayed reproductive growth (e.g., flowering later); enhanced seedling vigor (e.g., increased root biomass and length), enhanced lateral root formation and therefore soil penetration more extensive vascular system resulting in an enhanced transport system. 
     The plant can be any plant type including, for example, species from the genera  Cucurbita, Rosa, Vitis, Juglans, Fragaria, Lotus, Medicago, Onobrychis, Trifolium, Trigonella, Vigna, Citrus, Linum, Geranium, Manihot, Daucus, Arabidopsis, Brassica, Raphanus, Sinapis, Atropa, Capsicum, Datura, Hyoscyamus, Lycopersicon, Nicotiana, Solanum, Petunia, Digitalis, Majorana, Ciahorium, Helianthus, Lactuca, Bromus, Asparagus, Antirrhinum, Heterocallis, Nemesis, Pelargonium, Panieum, Pennisetum, Ranunculus, Senecio, Salpiglossis, Cucumis, Browaalia, Glycine, Pisum, Phaseolus, Lolium, Oryza, Zea, Avena, Hordeum, Secale, Triticum, Sorghum, Gossypium, Picea, Caco , and  Populus.    
     This invention provides a method of producing drought-tolerant plants comprising: preparing a nucleic acid construct which comprises a promoter operably-linked to a nucleic acid comprising or encoding antisense to SEQ ID NO: 1, 14, 40, 43, 80-85 or 172, or nucleic acid comprising a functional equivalent or fragment thereof of the antisense; inserting the nucleic acid construct into a vector; transforming a plant, tissue culture, or plant cells with the vector; and growing the plant or regenerating a plant from the tissue culture or plant cells; wherein drought-tolerant plants are produced. This method can be used wherein the nucleic acid is selected from the group consisting of 25-200 or more consecutive nucleotides complementary to SEQ ID NO: 1, 14, 40, 43, 80-85 or 172, oligonucleotides consisting of 25 or more consecutive nucleotides of SEQ ID NO: 1, 14, 40, 43, 80-85 or 172 or its complement, or nucleic acid encoding a peptide inhibitor of farnesyl transferase 
     In addition to stomatal regulation which is extremely sensitive to ABA, era plants also demonstrate delayed senescence under drought conditions, indicating that farnesylation negatively regulates a number of drought-induced responses in  Arabidopsis . The era plants grown under normal laboratory conditions take longer to turn yellow. The mutant plants remained green and viable long after the wild-type had senesced and died. Detached leaves of an era mutant plant do not yellow as quickly as detached leaves of wild-type plants ( FIG. 8 ). Similar-sized leaves which were developmentally identical were taken from wild-type and era plants and placed on agar-containing petri plates (See Example 7). Normally, a wild-type leaf begins to lose chlorophyll about five days later and eventually bleaches. The leaves of the mutant plants remained green for twice as long. Because the leaves were in constant contact with the agar they were not drought stressed, indicating the reduced senescence of the era1 mutant is not a drought-induced phenomenon. 
     Moreover, under a 10 hr day/16 hr night cycle, the plant life cycle can be doubled versus the wild-type plants (3 months). It appears therefore, that chlorophyll turnover and senescence signals are altered in the era1 mutant. For example, wild-type and mutant plants were grown in pots under well-watered conditions to stages of development where the wild-type plant leaves would begin to senesce (about the time of flower development). At this time, developmentally-similar leaves were assayed for senescence-induced marker genes by northern blot analysis (Example 8). Two genes, SAG12 and SAG13, in which transcription is normally induced during senescence in wild-type plants, were not induced in the era1 mutant ( FIG. 9 ). Further, CAB transcription is maintained ( FIG. 9 ). Taken together, these results indicate the senescence induction program in era1 mutants is delayed compared to wild-type plants, showing that loss of farnesylation activity causes a retardation of the induction of senescence in the plant even under conditions wherein water stress is not an environmental factor. 
     In addition to effects on senescence and water loss, the era1 mutants show a difference in branching and flowering habit when grown under diurnal light cycles. Under continuous (24 hours light/day) light, the branching pattern of mutants does not differ from that of wild-type plants. However, when given a dark period, the mutants do not produce as many lateral branches as wild-type plants. When measured, plants with loss of farnesylation activity produced only 2.4 branches per plant compared to 3.6 lateral branches per wild-type plant. This represents a 30% decrease in lateral branches per plant. 
     Flowering is affected by loss of Ftase activity as well. Plants lacking Ftase activity produce more flowers per plant (25-30 buds/inflorescence) than wild-type plants (10-15 buds/inflorescence). Thus, on average there are twice as many flower buds are present on the mutants than on the wild-type plants. 
     These pleiotrophic effects of the era1 loss of function mutants on whole plant development indicate that the ERA1 gene can be a coordinate regulator of a collection of plant developmental functions. 
     Until now, there was no known function for farnesylation in higher plants, including a role in ABA signal transduction. Ftases have been found in a number of higher plants such as tomato and pea, so it is clear that this enzyme has functions across species boundaries. Furthermore, overproduction of farnesyl transferase target peptides or the use of farnesylation inhibitors completely inactivates Ftase in mammalian and yeast systems. Thus, similar inhibitors can be applied to higher plants to inactivate Ftase in vivo. In both cases with the appropriate promoters, antisense Ftase or peptide inhibitors can be specifically targeted and controlled. 
     The farnesylation deficient mutants are also supersensitive to exogenous auxin. That these mutants show reduced branching and minor alterations in meristem organization, can be explained by altered auxin regulation. Thus other hormone functions are affected in this mutant, which indicates that, in addition to ABA pathways, other hormone regulated pathways are controlled by Ftase activity. These results demonstrate that the ERA1 gene provides a molecular mechanism to coordinate regulation of different hormone signaling molecules. 
     In accordance with the present invention, the plants included within the scope of this invention are higher and lower plants of the plant kingdom. Mature plants, seedlings and seeds are included in the scope of the invention. A mature plant includes a plant at any stage in development beyond the seedling. A seedling is a very young, immature plant in the early stages of development. Plant parts, protoplasts and tissue culture are also provided by this invention. 
     Transgenic plants are included within the scope of the present invention which have the phenotype characterized by the era1 mutation. Seed of transgenic plants are provided by this invention and can be used to propagate more plants containing the constructs of this invention. 
     ERA1 function in a number of crop plants can be inhibited to enhance the plant&#39;s response to adverse environmental conditions that require ABA-mediated signaling. Control of farnesylation in higher plants regulates both embryonic and vegetative tissue response to this hormone (Cutler, et al., 1996). The increased sensitivity translates into a faster response of the tissue to stress conditions which in turn confers increased protection of the plant to the environmental stress. Because this only requires the control of a single gene, ERA1, it should be possible to control farnesylation in a variety of plants by controlling the synthesis or activity of this enzyme. Furthermore, the work described herein clearly indicates that altering the ABA signal transduction pathway by manipulating the genes that control the ABA response makes it possible to improve the plant&#39;s response to adverse water stress conditions. 
     To produce transgenic plants of this invention, a construct comprising the gene encoding Ftase, or nucleic acid encoding its functional equivalent, and a promoter are incorporated into a vector through methods known and used by those of skill in the art. The promoter can comprise all or part of SEQ ID NO:3. The construct can also include any other necessary regulators such as terminators or the like, operably linked to the coding sequence. It can also be beneficial to include a 5′ leader sequence, such as the untranslated leader from the coat protein mRNA of alfalfa mosaic virus (Jobling, S. A. and Gehrke, L. (1987)  Nature  325:622-625) or the  maize  chlorotic mottle virus (MCMV) leader (Lommel, S. A., et al. (1991)  Virology  81:382-385). Those of skill in the art will recognize the applicability of other leader sequences for various purposes. Exemplary constructs include SEQ ID NO: 54-64. 
     Targeting sequences are also useful and can be incorporated into the constructs of this invention. A targeting sequence is usually translated into a peptide which directs the polypeptide product of the coding nucleic acid sequence to a desired location within the cell, such as to the plastid, and becomes separated from the peptide after transit of the peptide is complete or concurrently with transit. Examples of targeting sequences useful in this invention include, but are not limited to, the yeast mitochondrial presequence (Schmitz, et al. (1989)  Plant Cell  1:783-791), the targeting sequence from the pathogenesis-related gene (PR-1) of tobacco (Cornellisen, et al. (1986)  EMBO J.  5:37-40), vacuole targeting signals (Chrispeels, M. J. and Raikhel, N. V. (1992)  Cell  68:613-616), secretory pathway sequences such as those of the ER or Golgi (Chrispeels, M. J. (1991)  Ann. Rev. Plant Physiol. Plant Mol. Biol.  42:21-53). Intraorganellar sequences may also be useful for internal sites, e.g., thylakoids in chloroplasts. Theg, S. M. and Scott, S. V. (1993)  Trends in Cell Biol.  3:186-190. 
     In addition to 5′ leader sequences, terminater sequences are usually incorporated into the construct. In plant constructs, a 3′ untranslated region (3′ UTR) is generally part of the expression plasmid and contains a polyA termination sequence. The termination region which is employed will generally be one of convenience, since termination regions appear to be relatively interchangeable. The octopine synthase and nopaline synthase termination regions, derived from the Ti-plasmid of  A. tumefaciens , are suitable for such use in the constructs of this invention. 
     Any suitable technique can be used to introduce the nucleic acids and constructs of this invention to produce transgenic plants with an altered genome. For grasses such as  maize , microprojectile bombardment (see for example, Sanford, J. C., et al., U.S. Pat. No. 5,100,792 (1992) can be used. In this embodiment, a nucleotide construct or a vector containing the construct is coated onto small particles which are then introduced into the targeted tissue (cells) via high velocity ballistic penetration. The vector can be any vector which permits the expression of the exogenous DNA in plant cells into which the vector is introduced. The transformed cells are then cultivated under conditions appropriate for the regeneration of plants, resulting in production of transgenic plants. 
     Transgenic plants carrying the construct are examined for the desired phenotype using a variety of methods including but not limited to an appropriate phenotypic marker, such as antibiotic resistance or herbicide resistance, or visual observation of the time of floral induction compared to naturally-occurring plants. 
     Other known methods of inserting nucleic acid constructs into plants include  Agrobacterium -mediated transformation (see for example Smith, R. H., et al., U.S. Pat. No. 5,164,310 (1992)), electroporation (see for example, Calvin, N., U.S. Pat. No. 5,098,843 (1992)), introduction using laser beams (see for example, Kasuya, T., et al., U.S. Pat. No. 5,013,660 (1991)) or introduction using agents such as polyethylene glycol (see for example Golds, T. et al. (1993)  Biotechnology,  11:95-97), and the like. In general, plant cells may be transformed with a variety of vectors, such as viral, episomal vectors, Ti plasmid vectors and the like, in accordance with well known procedures. The method of introduction of the nucleic acid into the plant cell is not critical to this invention. 
     The methods of this invention can be used with in planta or seed transformation techniques which do not require culture or regeneration. Examples of these techniques are described in Bechtold, N., et al. (1993)  CR Acad. Sci. Paris/Life Sciences  316:118-93; Chang, S. S., et al. (1990)  Abstracts of the Fourth International Conference on Arabidopsis Research , Vienna, p. 28; Feldmann, K. A. and Marks, D. M (1987)  Mol. Gen. Genet.  208:1-9; Ledoux, L., et al. (1985)  Arabidopsis Inf. Serv.  22:1-11; Feldmann, K. A. (1992) In: Methods in  Arabidopsis  Research (Eds. Koncz, C., Chua, N-H, Schell, J.) pp. 274-289; Chee, et al., U.S. Pat. No. 5,376,543. 
     The transcriptional initiation region may provide for constitutive expression or regulated expression. In addition to the ERA1 promoter, many promoters are available which are functional in plants. 
     Constitutive promoters for plant gene expression include, but are not limited to, the octopine synthase, nopaline synthase, or mannopine synthase promoters from  Agrobacterium , the cauliflower mosaic virus (35S) promoter, the figwort mosaic virus (FMV) promoter, and the tobacco mosaic virus (TMV) promoter. Constitutive gene expression in plants can also be provided by the glutamine synthase promoter (Edwards, et al. (1990)  PNAS  87:3459-3463), the  maize  sucrose synthetase 1 promoter (Yang, et al. (1990)  PNAS  87:4144-4148), the promoter from the Rol-C gene of the TLDNA of Ri plasmid (Sagaya, et al. (1989)  Plant Cell Physiol.  30:649-654), and the phloem-specific region of the pRVC-S-3A promoter (Aoyagi, et al. (1988)  Mol. Gen. Genet.  213:179-185). 
     Heat-shock promoters, the ribulose-1,6-bisphosphate (RUBP) carboxylase small subunit (ssu) promoter, tissue specific promoters, and the like can be used for regulated expression of plant genes. Developmentally-regulated, stress-induced, wound-induced or pathogen-induced promoters are also useful. 
     The regulatory region may be responsive to a physical stimulus, such as light, as with the RUBP carboxylase ssu promoter, differentiation signals, or metabolites. The time and level of expression of the sense or antisense orientation can have a definite effect on the phenotype produced. Therefore, the promoters chosen, coupled with the orientation of the exogenous DNA, and site of integration of a vector in the genome, will determine the effect of the introduced gene. 
     Specific examples of regulated promoters also include, but are not limited to, the low temperature Kin1 and cor6.6 promoters (Wang, et al. (1995)  Plant Mol. Biol.  28:605; Wang, et al. (1995)  Plant Mol. Biol.  28:619-634), the ABA inducible promoter (Marcotte Jr., et al. (1989)  Plant Cell  1:969-976), heat shock promoters, such as the inducible hsp70 heat shock promoter of  Drosphilia melanogaster  (Freeling, M., et al. (1985)  Ann. Rev. of Genetics  19: 297-323), the cold inducible promoter from  B. napus  (White, T. C., et al. (1994)  Plant Physiol.  106:917), the alcohol dehydrogenase promoter which is induced by ethanol (Nagao, R. T., et al., Miflin, B. J., Ed.  Oxford Surveys of Plant Molecular and Cell Biology , Vol. 3, p 384-438, Oxford University Press, Oxford 1986), the phloem-specific sucrose synthase ASUS1 promoter from  Arabidopsis  (Martin, et al. (1993)  Plant J.  4:367-377), the ACS1 promoter (Rodrigues-Pousada, et al. (1993)  Plant Cell  5:897-911), the 22 kDa zein protein promoter from  maize  (Unger, et al. (1993)  Plant Cell  5:831-841), the ps1 lectin promoter of pea (de Pater, et al. (1993)  Plant Cell  5:877-886), the phas promoter from  Phaseolus vulgaris  (Frisch, et al. (1995)  Plant J.  7:503-512), the lea promoter (Thomas, T. L. (1993)  Plant Cell  5:1401-1410), the E8 gene promoter from tomato (Cordes, et al. (1989)  Plant Cell  1: 1025-1034), the PCNA promoter (Kosugi, et al. (1995)  Plant J.  7:877-886), the NTP303 promoter (Weterings, et al (1995)  Plant J.  8:55-63), the OSEM promoter (Hattori, et al. (1995)  Plant J.  7:913-925), the ADP GP promoter from potato (Muller-Rober, et al. (1994)  Plant Cell  6:601-604), the Myb promoter from barley (Wissenbach, et al. (1993)  Plant J.  4:411-422), and the plastocyanin promoter from  Arabidopsis  (Vorst, et al. (1993)  Plant J.  4:933-945). 
     The vector can be introduced into cells by a method appropriate to the type of host cells (e.g., transformation, electroporation, transfection). For the purposes of this disclosure, the terms “transformed with”, “transformant”, “transformation”, “transfect with”, and “transfection” all refer to the introduction of a nucleic acid into a cell by one of the numerous methods known to persons skilled in the art. Transformation of prokaryotic cells, for example, is commonly achieved by treating the cells with calcium chloride so as to render them “competent” to take up exogenous DNA, and then mixing such DNA with the competent cells. Prokaryotic cells can also be infected with a recombinant bacteriophage vector. 
     Nucleic acids can be introduced into cells of higher organisms by viral infection, bacteria-mediated transfer (e.g.,  Agrobacterium  T-DNA delivery system), electroporation, calcium phosphate co-precipitation, microinjection, lipofection, bombardment with nucleic-acid coated particles or other techniques, depending on the particular cell type. For grasses such as corn and sorghum, microprojectile bombardment as described, for example, in Sanford, J. C., et al., U.S. Pat. No. 5,100,792 (1992) can be used. Other useful protocols for the transformation of plant cells are provided in Gelvin et al., 1992. Suitable protocols for transforming and transfecting cells are also found in Sambrook et al., 1989. The nucleic acid constructs of this invention can also be incorporated into specific plant parts such as those described supra through the transformation and transfection techniques described herein. 
     To aid in identification of transformed plant cells, the constructs of this invention are further manipulated to include genes coding for plant selectable markers. Useful selectable markers include enzymes which provide for resistance to an antibiotic such as gentamycin, hygromycin, kanamycin, or the like. Similarly, enzymes providing for production of a compound identifiable by color change such as GUS (β-glucuronidase), or by luminescence, such as luciferase, are useful. 
     For example, antisense Ftase can be produced by integrating a complement of the ERA1 gene linked to DNA comprising SEQ ID NO:3 into the genome of a virus that enters the host cells. By infection of the host cells, the components of a system which permits the transcription of the antisense present in the host cells. 
     When cells or protoplasts containing the antisense gene driven by a promoter of the present invention are obtained, the cells or protoplasts are regenerated into whole plants. The transformed cells are then cultivated under conditions appropriate for the regeneration of plants, resulting in production of transgenic plants. Choice of methodology for the regeneration step is not critical, with suitable protocols being available for many varieties of plants, tissues and other photosynthetic organisms. See, e.g., Gelvin S. B. and Schilperoort R. A., eds.  Plant Molecular Biology Manual, Second Edition , Suppl. 1 (1995) Kluwer Academic Publishers, Boston Mass., U.S.A. 
     Transgenic plants carrying the construct are examined for the desired phenotype using a variety of methods including but not limited to an appropriate phenotypic marker, such as antibiotic resistance or herbicide resistance as described supra, or visual observation of their growth compared to the growth of the naturally-occurring plants under the same conditions. 
     As used herein, the term transgenic plants includes plants that contain either DNA or RNA which does not naturally occur in the wild type (native) plant or known variants, or additional or inverted copies of the naturally-occurring DNA and which is introduced as described herein. Transgenic plants include those into which isolated nucleic acids have been introduced and their descendants, produced from seed, vegetative propagation, cell, tissue or protoplast culture, or the like wherein such alteration is maintained. 
     Such transgenic plants include, in one embodiment, transgenic plants which are angiosperms, both monocotyledons and dicotyledons. Transgenic plants include those into which DNA has been introduced and their progeny, produced from seed, vegetative propagation, cell, tissue or protoplast culture, or the like. 
     Seed can be obtained from the regenerated plant or from a cross between the regenerated plant and a suitable plant of the same species. Alternatively, the plant can be vegetatively propagated by culturing plant parts under conditions suitable for the regeneration of such plant parts. 
     In yet another aspect of this invention are provided plant tissue culture and protoplasts which contain DNA comprising antisense or an altered ERA1 nucleic acid operably linked to an ERA1 promoter, which alters the response of the tissue culture or protoplasts to varying environmental conditions. 
     The methods of this invention can also be used with in planta or seed transformation techniques which do not require culture or regeneration. Examples of these techniques are described in Bechtold, N., et al. (1993)  CR Acad. Sci. Paris/Life Sciences  316:118-93; Chang, S. S., et al. (1990)  Abstracts of the Fourth International Conference on Arabidopsis Research , Vienna, p. 28; Feldmann, K. A. and Marks, D. M (1987)  Mol. Gen. Genet.  208:1-9; Ledoux, L., et al. (1985)  Arabidopsis Inf. Serv.  22:1-11; Feldmann, K. A. (1992) In: Methods in  Arabidopsis  Research (Eds. Koncz, C., Chua, N-H, Schell, J.) pp. 274-289; Chee, et al., U.S. Pat. No. 5,376,543. 
     The isolated nucleic acid molecules of the invention can be used to express PPI protein (e.g., via a recombinant expression vector in a host cell), to detect PPI mRNA (e.g., in a biological sample) or a genetic lesion in a PPI gene, and to modulate PPI activity, as described further, below. In addition, the PPI proteins can be used to screen compounds that modulate the PPI protein activity or expression. In addition, the anti-PPI antibodies of the invention can be used to detect and isolate PPI proteins and modulate PPI activity. 
     The invention provides a method (also referred to herein as a “screening assay”) for identifying modulators, i.e., candidate or test compounds or agents (e.g., peptides, peptidomimetics, small molecules or other drugs) that bind to PPI proteins or have a stimulatory or inhibitory effect on, e.g., PPI protein expression or PPI protein activity. The invention also includes compounds identified in the screening assays described herein. 
     In one embodiment, the invention provides assays for screening candidate or test compounds which bind to a PPI protein or polypeptide or biologically-active portion thereof. The test compounds of the invention can be obtained using any of the numerous approaches in combinatorial library methods known in the art, including: biological libraries; spatially addressable parallel solid phase or solution phase libraries; synthetic library methods requiring deconvolution; the “one-bead one-compound” library method; and synthetic library methods using affinity chromatography selection. The biological library approach is limited to peptide libraries, while the other four approaches are applicable to peptide, non-peptide oligomer or small molecule libraries of compounds. See, e.g., Lam, 1997 . Anticancer Drug Design  12: 145. A “small molecule” as used herein, is meant to refer to a composition that has a molecular weight of less than about 5 kD and most preferably less than about 4 kD. Small molecules can be, e.g., nucleic acids, peptides, polypeptides, peptidomimetics, carbohydrates, lipids or other organic or inorganic molecules. Libraries of chemical and/or biological mixtures, such as fungal, bacterial, or algal extracts, are known in the art and can be screened with any of the assays of the invention. 
     Examples of methods for the synthesis of molecular libraries can be found in the art, for example in: DeWitt, et al., 1993 . Proc. Natl. Acad. Sci. U.S.A.  90: 6909; Erb, et al., 1994 . Proc. Natl. Acad. Sci. U.S.A.  91: 11422; Zuckermann, et al., 1994 . J. Med. Chem.  37: 2678; Cho, et al., 1993 . Science  261: 1303; Carrell, et al., 1994 . Angew. Chem. Int. Ed. Engl.  33: 2059; Carell, et al., 1994 . Angew. Chem. Int. Ed. Engl.  33: 2061; and Gallop, et al., 1994 . J. Med. Chem.  37: 1233. 
     Libraries of compounds may be presented in solution (e.g., Houghten, 1992 . Biotechniques  13: 412-421), or on beads (Lam, 1991 . Nature  354: 82-84), on chips (Fodor, 1993 . Nature  364: 555-556), bacteria (Ladner, U.S. Pat. No. 5,223,409), spores (Ladner, U.S. Pat. No. 5,233,409), plasmids (Cull, et al., 1992 . Proc. Natl. Acad. Sci. USA  89: 1865-1869) or on phage (Scott and Smith, 1990 . Science  249: 386-390; Devlin, 1990 . Science  249: 404-406; Cwirla, et al., 1990 . Proc. Natl. Acad. Sci. U.S.A.  87: 6378-6382; Felici, 1991 . J. Mol. Biol.  222: 301-310; Ladner, U.S. Pat. No. 5,233,409). 
     In one embodiment, an assay is a cell-based assay in which a cell which expresses a PPI protein, or a biologically-active portion thereof, is contacted with a test compound and the ability of the test compound to bind to a PPI protein determined. The cell, for example, can be of mammalian origin, plant cell or a yeast cell. Determining the ability of the test compound to bind to the PPI protein can be accomplished, for example, by coupling the test compound with a radioisotope or enzymatic label such that binding of the test compound to the PPI protein or biologically-active portion thereof can be determined by detecting the labeled compound in a complex. For example, test compounds can be labeled with  125 I,  35 S,  14 C, or  3 H, either directly or indirectly, and the radioisotope detected by direct counting of radioemission or by scintillation counting. Alternatively, test compounds can be enzymatically-labeled with, for example, horseradish peroxidase, alkaline phosphatase, or luciferase, and the enzymatic label detected by determination of conversion of an appropriate substrate to product. In one embodiment, the assay comprises contacting a cell which expresses a PPI protein, or a biologically-active portion thereof, with a known compound which binds PPI to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with a PPI protein, wherein determining the ability of the test compound to interact with a PPI protein comprises determining the ability of the test compound to preferentially bind to PPI protein or a biologically-active portion thereof as compared to the known compound. 
     In another embodiment, an assay is a cell-based assay comprising contacting a cell expressing a PPI protein, or a biologically-active portion thereof, with a test compound and determining the ability of the test compound to modulate (e.g., stimulate or inhibit) the activity of the PPI protein or biologically-active portion thereof. Determining the ability of the test compound to modulate the activity of PPI or a biologically-active portion thereof can be accomplished, for example, by determining the ability of the PPI protein to bind to or interact with a PPI target molecule. As used herein, a “target molecule” is a molecule with which a PPI protein binds or interacts in nature, for example, a molecule on the surface of a cell which expresses a PPI interacting protein, a molecule on the surface of a second cell, a molecule in the extracellular milieu, a molecule associated with the internal surface of a cell membrane or a cytoplasmic molecule. A PPI target molecule can be a non-PPI molecule or a PPI protein or polypeptide of the invention In one embodiment, a PPI target molecule is a component of a signal transduction pathway that facilitates transduction of an extracellular signal (e.g. a signal generated by binding of a compound to a membrane-bound molecule) through the cell membrane and into the cell. The target, for example, can be a second intercellular protein that has catalytic activity or a protein that facilitates the association of downstream signaling molecules with PPI. 
     Determining the ability of the PPI protein to bind to or interact with a PPI target molecule can be accomplished by one of the methods described above for determining direct binding. In one embodiment, determining the ability of the PPI protein to bind to or interact with a PPI target molecule can be accomplished by determining the activity of the target molecule. For example, the activity of the target molecule can be determined by detecting induction of a cellular second messenger of the target (i.e. intracellular Ca 2+ , diacylglycerol, IP 3 , etc.), detecting catalytic/enzymatic activity of the target an appropriate substrate, detecting the induction of a reporter gene (comprising a PPI-responsive regulatory element operatively linked to a nucleic acid encoding a detectable marker, e.g., luciferase), or detecting a cellular response, for example, cell survival, cellular differentiation, or cell proliferation. 
     In yet another embodiment, an assay of the invention is a cell-free assay comprising contacting a PPI protein or biologically-active portion thereof with a test compound and determining the ability of the test compound to bind to the PPI protein or biologically-active portion thereof. Binding of the test compound to the PPI protein can be determined either directly or indirectly as described above. In one such embodiment, the assay comprises contacting the PPI protein or biologically-active portion thereof with a known compound which binds PPI to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with a PPI protein, wherein determining the ability of the test compound to interact with a PPI protein comprises determining the ability of the test compound to preferentially bind to PPI or biologically-active portion thereof as compared to the known compound. 
     In still another embodiment, an assay is a cell-free assay comprising contacting PPI protein or biologically-active portion thereof with a test compound and determining the ability of the test compound to modulate (e.g. stimulate or inhibit) the activity of the PPI protein or biologically-active portion thereof. Determining the ability of the test compound to modulate the activity of PPI can be accomplished, for example, by determining the ability of the PPI protein to bind to a PPI target molecule by one of the methods described above for determining direct binding. In an alternative embodiment, determining the ability of the test compound to modulate the activity of PPI protein can be accomplished by determining the ability of the PPI protein further modulate a PPI target molecule. For example, the catalytic/enzymatic activity of the target molecule on an appropriate substrate can be determined as described above. 
     In yet another embodiment, the cell-free assay comprises contacting the PPI protein or biologically-active portion thereof with a known compound which binds PPI protein to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with a PPI protein, wherein determining the ability of the test compound to interact with a PPI protein comprises determining the ability of the PPI protein to preferentially bind to or modulate the activity of a PPI target molecule. 
     The cell-free assays of the invention are amenable to use of either the soluble form or the membrane-bound form of PPI protein. In the case of cell-free assays comprising the membrane-bound form of PPI protein, it may be desirable to utilize a solubilizing agent such that the membrane-bound form of PPI protein is maintained in solution. Examples of such solubilizing agents include non-ionic detergents such as n-octylglucoside, n-dodecylglucoside, n-dodecylmaltoside, octanoyl-N-methylglucamide, decanoyl-N-methylglucamide, Triton® X-100, Triton® X-114, Thesit®, Isotridecypoly (ethylene glycol ether) n , N-dodecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate, 3-(3-cholamidopropyl) dimethylamminiol-1-propane sulfonate (CHAPS), or 3-(3-cholamidopropyl)dimethylamminiol-2-hydroxy-1-propane sulfonate (CHAPSO). 
     In more than one embodiment of the above assay methods of the invention, it may be desirable to immobilize either PPI protein or its target molecule to facilitate separation of complexed from uncomplexed forms of one or both of the proteins, as well as to accommodate automation of the assay. Binding of a test compound to PPI protein, or interaction of PPI protein with a target molecule in the presence and absence of a candidate compound, can be accomplished in any vessel suitable for containing the reactants. Examples of such vessels include microtiter plates, test tubes, and micro-centrifuge tubes. In one embodiment, a fusion protein can be provided that adds a domain that allows one or both of the proteins to be bound to a matrix. For example, GST-PPI fusion proteins or GST-target fusion proteins can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St. Louis, Mo.) or glutathione derivatized microtiter plates, that are then combined with the test compound or the test compound and either the non-adsorbed target protein or PPI protein, and the mixture is incubated under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the beads or microtiter plate wells are washed to remove any unbound components, the matrix immobilized in the case of beads, complex determined either directly or indirectly, for example, as described, supra. Alternatively, the complexes can be dissociated from the matrix, and the level of PPI protein binding or activity determined using standard techniques. 
     Other techniques for immobilizing proteins on matrices can also be used in the screening assays of the invention. For example, either the PPI protein or its target molecule can be immobilized utilizing conjugation of biotin and streptavidin. Biotinylated PPI protein or target molecules can be prepared from biotin-NHS(N-hydroxy-succinimide) using techniques well-known within the art (e.g., biotinylation kit, Pierce Chemicals, Rockford, Ill.), and immobilized in the wells of streptavidin-coated 96 well plates (Pierce Chemical). Alternatively, antibodies reactive with PPI protein or target molecules, but which do not interfere with binding of the PPI protein to its target molecule, can be derivatized to the wells of the plate, and unbound target or PPI protein trapped in the wells by antibody conjugation. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the PPI protein or target molecule, as well as enzyme-linked assays that rely on detecting an enzymatic activity associated with the PPI protein or target molecule. 
     In another embodiment, modulators of PPI protein expression are identified in a method wherein a cell is contacted with a candidate compound and the expression of PPI mRNA or protein in the cell is determined. The level of expression of PPI mRNA or protein in the presence of the candidate compound is compared to the level of expression of PPI mRNA or protein in the absence of the candidate compound. The candidate compound can then be identified as a modulator of PPI mRNA or protein expression based upon this comparison. For example, when expression of PPI mRNA or protein is greater (i.e., statistically significantly greater) in the presence of the candidate compound than in its absence, the candidate compound is identified as a stimulator of PPI mRNA or protein expression. Alternatively, when expression of PPI mRNA or protein is less (statistically significantly less) in the presence of the candidate compound than in its absence, the candidate compound is identified as an inhibitor of PPI mRNA or protein expression. The level of PPI mRNA or protein expression in the cells can be determined by methods described herein for detecting PPI mRNA or protein. 
     In yet another aspect of the invention, the PPI proteins can be used as “bait proteins” in a two-hybrid assay or three hybrid assay (see, e.g., U.S. Pat. No. 5,283,317; Zervos, et al., 1993 . Cell  72: 223-232; Madura, et al., 1993 . J. Biol. Chem.  268: 12046-12054; Bartel, et al., 1993 . Biotechniques  14: 920-924; Iwabuchi, et al., 1993 . Oncogene  8: 1693-1696; and Brent WO 94/10300), to identify other proteins that bind to or interact with PPI (“PPI-binding proteins” or “PPI-bp”) and modulate PPI activity. Such PPI-binding proteins are also likely to be involved in the propagation of signals by the PPI proteins as, for example, upstream or downstream elements of the PPI pathway. 
     The two-hybrid system is based on the modular nature of most transcription factors, which consist of separable DNA-binding and activation domains. Briefly, the assay utilizes two different DNA constructs. In one construct, the gene that codes for PPI is fused to a gene encoding the DNA binding domain of a known transcription factor (e.g., GAL-4). In the other construct, a DNA sequence, from a library of DNA sequences, that encodes an unidentified protein (“prey” or “sample”) is fused to a gene that codes for the activation domain of the known transcription factor. If the “bait” and the “prey” proteins are able to interact, in vivo, forming a PPI-dependent complex, the DNA-binding and activation domains of the transcription factor are brought into close proximity. This proximity allows transcription of a reporter gene (e.g., LacZ) that is operably linked to a transcriptional regulatory site responsive to the transcription factor. Expression of the reporter gene can be detected and cell colonies containing the functional transcription factor can be isolated and used to obtain the cloned gene that encodes the protein which interacts with PPI. 
     In yet another aspect of the invention are methods which utilize the transgenic plants of the invention to identify PPI-interacting components via genetic screening protocols. These components can be for example, regulatory elements which modify PPI-gene expression, interacting proteins which directly modify PPI activity or interacting proteins which modify components of the same signal transduction pathway and thereby exert an effect on the expression or activity of PPI. Briefly, genetic screening protocols are applied to the transgenic plants of the invention and in so doing identify related genes which are not identified using a wild type background for the screen. For example an activation tagged library (Weigel, et al., 2000 . Plant Physiol.  122: 1003-1013), can be produced using the transgenic plants of the invention as the genetic background. Plants are then screened for altered phenotypes from that displayed by the parent plants. Alternative methods of generating libraries from the transgenic plants of the invention can be used, for example, chemical or irradiation induced mutations, insertional inactivation or insertional activation methods. 
     The invention further pertains to novel agents identified by the aforementioned screening assays and uses thereof. 
     Recombinant Expression Vectors and Host Cells 
     Another aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding a PPI protein, or derivatives, fragments, analogs or homologs thereof. As used herein, the term “vector” refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. Exemplary expression vector constructs include for example the constructs of SEQ ID NO: 54-64. One type of vector is a “plasmid”, which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication). Other vectors are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively-linked. Such vectors are referred to herein as “expression vectors”. In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, “plasmid” and “vector” can be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors or plant transformation vectors, binary or otherwise, which serve equivalent functions. 
     The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, that is operatively-linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, “operably-linked” is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner that allows for expression of the nucleotide sequence (e.g., in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell). 
     The term “regulatory sequence” is intended to include promoters, enhancers and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel, G ENE  E XPRESSION  T ECHNOLOGY : M ETHODS IN  E NZYMOLOGY  185, Academic Press, San Diego, Calif. (1990). Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cell and those that direct expression of the nucleotide sequence only in certain host cells (e.g., tissue-specific regulatory sequences). Examples of suitable promoters include for example constitutive promoters, ABA inducible promoters, tissue specific promoters or guard cell specific promoters. It will be appreciated by those skilled in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein (e.g., PPI proteins, mutant forms of PPI proteins, fusion proteins, etc.). 
     The recombinant expression vectors of the invention can be designed for expression of PPI proteins in prokaryotic or eukaryotic cells. For example, PPI proteins can be expressed in bacterial cells such as  Escherichia coli , insect cells (using baculovirus expression vectors) yeast cells, plant cells or mammalian cells. Suitable host cells are discussed further in Goeddel, G ENE  E XPRESSION  T ECHNOLOGY : M ETHODS IN  E NZYMOLOGY  185, Academic Press, San Diego, Calif. (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase. 
     Expression of proteins in prokaryotes is most often carried out in  Escherichia coli  with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein encoded therein, usually to the amino terminus of the recombinant protein. Such fusion vectors typically serve three purposes: (i) to increase expression of recombinant protein; (ii) to increase the solubility of the recombinant protein; and (iii) to aid in the purification of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase. Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith and Johnson, 1988 . Gene  67: 31-40), pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) that fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. 
     Examples of suitable inducible non-fusion  E. coli  expression vectors include pTrc (Amrann et al., (1988)  Gene  69:301-315) and pET 11d (Studier et al., G ENE  E XPRESSION  T ECHNOLOGY : M ETHODS IN  E NZYMOLOGY  185, Academic Press, San Diego, Calif. (1990) 60-89). 
     One strategy to maximize recombinant protein expression in  E. coli  is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein. See, e.g., Gottesman, G ENE  E XPRESSION  T ECHNOLOGY : M ETHODS IN  E NZYMOLOGY  185, Academic Press, San Diego, Calif. (1990) 119-128. Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in  E. coli  (see, e.g., Wada, et al., 1992 . Nucl. Acids Res.  20: 2111-2118). Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques. 
     In another embodiment, the PPI expression vector is a yeast expression vector. Examples of vectors for expression in yeast  Saccharomyces cerivisae  include pYepSec1 (Baldari, et al., 1987 . EMBO J.  6: 229-234), pMFa (Kurjan and Herskowitz, 1982 . Cell  30: 933-943), pJRY88 (Schultz et al., 1987 . Gene  54: 113-123), pYES2 (Invitrogen Corporation, San Diego, Calif.), and picZ (InVitrogen Corp, San Diego, Calif.). 
     Alternatively, PPI can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells (e.g., SF9 cells) include the pAc series (Smith, et al., 1983 . Mol. Cell. Biol.  3: 2156-2165) and the pVL series (Lucklow and Summers, 1989 . Virology  170: 31-39). 
     In yet another embodiment, a nucleic acid of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, 1987 . Nature  329: 840) and pMT2PC (Kaufman, et al., 1987 . EMBO J.  6: 187-195). When used in mammalian cells, the expression vector&#39;s control functions are often provided by viral regulatory elements. For example, commonly used promoters are derived from polyoma, adenovirus 2, cytomegalovirus, and simian virus 40. For other suitable expression systems for both prokaryotic and eukaryotic cells see, e.g., Chapters 16 and 17 of Sambrook, et al., M OLECULAR  C LONING : A L ABORATORY  M ANUAL.  2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989. 
     In yet another embodiment, a nucleic acid of the invention is expressed in plants cells using a plant expression vector. Examples of plant expression vectors systems include tumor inducing (Ti) plasmid or portion thereof found in  Agrobacterium , cauliflower mosaic virus (CAMV) DNA and vectors such as pBI121. 
     For expression in plants, the recombinant expression cassette will contain in addition to the PPI nucleic acids, a plant promoter region, a transcription initiation site (if the coding sequence to transcribed lacks one), and a transcription termination/polyadenylation sequence. The termination/polyadenylation region may be obtained from the same gene as the promoter sequence or may be obtained from different genes. Unique restriction enzyme sites at the 5′ and 3′ ends of the cassette are typically included to allow for easy insertion into a pre-existing vector. Examples of suitable promoters include promoters from plant viruses such as the 35S promoter from cauliflower mosaic virus (CaMV). Odell, et al., Nature, 313: 810-812 (1985). and promoters from genes such as rice actin (McElroy, et al., Plant Cell, 163-171 (1990)); ubiquitin (Christensen, et al., Plant Mol. Biol., 12: 619-632 (1992); and Christensen, et al., Plant Mol. Biol., 18: 675-689 (1992)); pEMU (Last, et al., Theor. Appl. Genet., 81: 581-588 (1991)); MAS (Velten, et al., EMBO J., 3: 2723-2730 (1984));  maize  H3 histone (Lepetit, et al., Mol. Gen. Genet., 231: 276-285 (1992); and Atanassvoa, et al., Plant Journal, 2(3): 291-300 (1992)), the 5′- or 3′-promoter derived from T-DNA of  Agrobacterium tumefaciens , the Smas promoter, the cinnamyl alcohol dehydrogenase promoter (U.S. Pat. No. 5,683,439), the Nos promoter, the rubisco promoter, the GRP1-8 promoter, ALS promoter, (WO 96/30530), a synthetic promoter, such as, Rsyn7, SCP and UCP promoters, ribulose-1,3-diphosphate carboxylase, fruit-specific promoters, heat shock promoters, seed-specific promoters and other transcription initiation regions from various plant genes, for example, include the various opine initiation regions, such as for example, octopine, mannopine, and nopaline. 
     Additional regulatory elements that may be connected to a PPI encoding nucleic acid sequence for expression in plant cells include terminaters, polyadenylation sequences, and nucleic acid sequences encoding signal peptides that permit localization within a plant cell or secretion of the protein from the cell. Such regulatory elements and methods for adding or exchanging these elements with the regulatory elements PPI gene are known, and include, but are not limited to, 3′ termination and/or polyadenylation regions such as those of the  Agrobacterium tumefaciens  nopaline synthase (nos) gene (Bevan, et al., Nucl. Acids Res., 12: 369-385 (1983)); the potato proteinase inhibitor II (PINII) gene (Keil, et al., Nucl. Acids Res., 14: 5641-5650 (1986) and hereby incorporated by reference); and An, et al., Plant Cell, 1: 115-122 (1989)); and the CaMV 19S gene (Mogen, et al., Plant Cell, 2: 1261-1272 (1990)). 
     Plant signal sequences, including, but not limited to, signal-peptide encoding DNA/RNA sequences which target proteins to the extracellular matrix of the plant cell (Dratewka-Kos, et al., J. Biol. Chem., 264: 4896-4900 (1989)) and the  Nicotiana plumbaginifolia  extension gene (DeLoose, et al., Gene, 99: 95-100 (1991)), or signal peptides which target proteins to the vacuole like the sweet potato sporamin gene (Matsuka, et al., Proc. Nat&#39;l Acad. Sci. (USA), 88: 834 (1991)) and the barley lectin gene (Wilkins, et al., Plant Cell, 2: 301-313 (1990)), or signals which cause proteins to be secreted such as that of PRIb (Lind, et al., Plant Mol. Biol., 18: 47-53 (1992)), or those which target proteins to the plastids such as that of rapeseed enoyl-ACP reductase (Verwaert, et al., Plant Mol. Biol., 26: 189-202 (1994)) are useful in the invention. 
     In another embodiment, the recombinant expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type (e.g., tissue-specific regulatory elements are used to express the nucleic acid). Tissue-specific regulatory elements are known in the art. Especially useful in connection with the nucleic acids of the present invention are expression systems which are operable in plants. These include systems which are under control of a tissue-specific promoter, as well as those which involve promoters that are operable in all plant tissues. 
     Organ-specific promoters are also well known. For example, the patatin class I promoter is transcriptionally activated only in the potato tuber and can be used to target gene expression in the tuber (Bevan, M., 1986,  Nucleic Acids Research  14:4625-4636). Another potato-specific promoter is the granule-bound starch synthase (GBSS) promoter (Visser, R. G. R, et al, 1991,  Plant Molecular Biology  17:691-699). 
     Other organ-specific promoters appropriate for a desired target organ can be isolated using known procedures. These control sequences are generally associated with genes uniquely expressed in the desired organ. In a typical higher plant, each organ has thousands of mRNAs that are absent from other organ systems (reviewed in Goldberg, P., 1986,  Trans. R. Soc. London B 314:343). 
     For in situ production of the antisense mRNA of GST, those regions of the GST gene which are transcribed into GST mRNA, including the untranslated regions thereof, are inserted into the expression vector under control of the promoter system in a reverse orientation. The resulting transcribed mRNA is then complementary to that normally produced by the plant. 
     The resulting expression system or cassette is ligated into or otherwise constructed to be included in a recombinant vector which is appropriate for plant transformation. The vector may also contain a selectable marker gene by which transformed plant cells can be identified in culture. Usually, the marker gene will encode antibiotic resistance. These markers include resistance to G418, hygromycin, bleomycin, kanamycin, and gentamicin. After transforming the plant cells, those cells having the vector will be identified by their ability to grow on a medium containing the particular antibiotic. Replication sequences, of bacterial or viral origin, are generally also included to allow the vector to be cloned in a bacterial or phage host, preferably a broad host range prokaryotic origin of replication is included. A selectable marker for bacteria should also be included to allow selection of bacterial cells bearing the desired construct. Suitable prokaryotic selectable markers also include resistance to antibiotics such as kanamycin or tetracycline. 
     Other DNA sequences encoding additional functions may also be present in the vector, as is known in the art. For instance, in the case of  Agrobacterium  transformations, T-DNA sequences will also be included for subsequent transfer to plant chromosomes. 
     Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms “host cell” and “recombinant host cell” are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but also to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein. 
     Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms “transformation” and “transfection” are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid (e.g., DNA) into a host cell. 
     A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce (i.e., express) a polypeptide of the invention encoded in a an open reading frame of a polynucleotide of the invention. Accordingly, the invention further provides methods for producing a polypeptide using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding a polypeptide of the invention has been introduced) in a suitable medium such that the polypeptide is produced. In another embodiment, the method further comprises isolating the polypeptide from the medium or the host cell. 
     A number of types of cells may act as suitable host cells for expression of a polypeptide encoded by an open reading frame in a polynucleotide of the invention. Plant host cells include, for example, plant cells that could function as suitable hosts for the expression of a polynucleotide of the invention include epidermal cells, mesophyll and other ground tissues, and vascular tissues in leaves, stems, floral organs, and roots from a variety of plant species, such as  Arabidopsis thaliana, Nicotiana tabacum, Brassica napus, Zea mays, Oryza sativa, Gossypium hirsutum  and  Glycine max.    
     Alternatively, it may be possible to produce a polypeptide in lower eukaryotes such as yeast or in prokaryotes such as bacteria. Potentially suitable yeast strains include  Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces strains, Candida , or any yeast strain capable of expressing heterologous proteins. Potentially suitable bacterial strains include  Escherichia coli, Bacillus subtilis, Salmonella typhimurium , or any bacterial strain capable of expressing heterologous polypeptides. If the polypeptide is made in yeast or bacteria, it may be necessary to modify the polypeptide produced therein, for example by phosphorylation or glycosylation of the appropriate sites, in order to obtain a functional polypeptide, if the polypeptide is of sufficient length and conformation to have activity. Such covalent attachments may be accomplished using known chemical or enzymatic methods. 
     A polypeptide may be prepared by culturing transformed host cells under culture conditions suitable to express the recombinant protein. The resulting expressed polypeptide or protein may then be purified from such culture (e.g., from culture medium or cell extracts) using known purification processes, such as gel filtration and ion exchange chromatography. The purification of the polypeptide or protein may also include an affinity column containing agents which will bind to the protein; one or more column steps over such affinity resins as concanavalin A-agarose, Heparin-Toyopearl® or Cibacrom blue 3GA Sepharose®; one or more steps involving hydrophobic interaction chromatography using such resins as phenyl ether, butyl ether, or propyl ether; or immunoaffinity chromatography. 
     Alternatively, a polypeptide or protein may also be expressed in a form which will facilitate purification. For example, it may be expressed as a fusion protein containing a six-residue histidine tag. The histidine-tagged protein will then bind to a Ni-affinity column. After elution of all other proteins, the histidine-tagged protein can be eluted to achieve rapid and efficient purification. One or more reverse-phase high performance liquid chromatography (RP-HPLC) steps employing hydrophobic RP-HPLC media, e.g., silica gel having pendant methyl or other aliphatic groups, can be employed to further purify a polypeptide. Some or all of the foregoing purification steps, in various combinations, can also be employed to provide a substantially homogeneous isolated recombinant polypeptide. The protein or polypeptide thus purified is substantially free of other plant proteins or polypeptides and is defined in accordance with the present invention as “isolated.” 
     Transformed Plants Cells and Transgenic Plants 
     The invention includes protoplast, plants cells, plant tissue and plants (e.g., monocots and dicots transformed with a PPI nucleic acid (i.e, sense or antisense), a vector containing a PPI nucleic acid (i.e, sense or antisense) or an expression vector containing a PPI nucleic acid (i.e, sense or antisense). As used herein, “plant” is meant to include not only a whole plant but also a portion thereof (i.e., cells, and tissues, including for example, leaves, stems, shoots, roots, flowers, fruits and seeds). 
     The plant can be any plant type including, for example, species from the genera  Cucurbita, Rosa, Vitis, Juglans, Fragaria, Lotus, Medicago, Onobrychis, Trifolium, Trigonella, Vigna, Citrus, Linum, Geranium, Manihot, Daucus, Arabidopsis, Brassica, Raphanus, Sinapis, Atropa, Capsicum, Datura, Hyoscyamus, Lycopersicon, Nicotiana, Solanum, Petunia, Digitalis, Majorana, Ciahorium, Helianthus, Lactuca, Bromus, Asparagus, Antirrhinum, Heterocallis, Nemesis, Pelargonium, Panieum, Pennisetum, Ranunculus, Senecio, Salpiglossis, Cucumis, Browaalia, Glycine, Pisum, Phaseolus, Lolium, Oryza, Zea, Avena, Hordeum, Secale, Triticum, Sorghum, Gossypium, Picea, Caco , and  Populus.    
     In some aspects of the invention, the transformed plant is resistant to biotic and abiotic stresses, e.g., chilling stress, salt stress, water stress (e.g., drought), disease, grazing pests and wound healing. Additionally, the invention also includes a transgenic plant that is resistant to pathogens such as for example fungi, bacteria, nematodes, viruses and parasitic weeds. Alternatively, the transgenic plant is resistant to herbicides or has delayed senescence. The transgenic plant has an increase in yield, productivity, biomass or ABA sensitivity. By resistant is meant the plant grows under stress conditions (e.g., high salt, decreased water, low temperatures) or under conditions that normally inhibit, to some degree, the growth of an untransformed plant. Methodologies to determine plant growth or response to stress include for example, height measurements, weight measurements, leaf area, ability to flower, water use, transpiration rates and yield. 
     The invention also includes cells, tissues, including for example, leaves, stems, shoots, roots, flowers, fruits and seeds and the progeny derived from the transformed plant. 
     Numerous methods for introducing foreign genes into plants are known and can be used to insert a gene into a plant host, including biological and physical plant transformation protocols. See, for example, Miki et al., (1993) “Procedure for Introducing Foreign DNA into Plants”, In: Methods in Plant Molecular Biology and Biotechnology, Glick and Thompson, eds., CRC Press, Inc., Boca Raton, pages 67-88 and Andrew Bent in, Clough S J and Bent A F, 1998. Floral dipping: a simplified method for  Agrobacterium -mediated transformation of  Arabidopsis thaliana . The methods chosen vary with the host plant, and include chemical transfection methods such as calcium phosphate, polyethylene glycol (PEG) transformation, microorganism-mediated gene transfer such as  Agrobacterium  (Horsch, et al., Science, 227: 1229-31 (1985)), electroporation, protoplast transformation, micro-injection, flower dipping and biolistic bombardment. 
       Agrobacterium -Mediated Transformation 
     The most widely utilized method for introducing an expression vector into plants is based on the natural transformation system of  Agrobacterium. A. tumefaciens  and  A. rhizogenes  are plant pathogenic soil bacteria which genetically transform plant cells. The Ti and Ri plasmids of  A. tumefaciens  and  A. rhizogenes , respectfully, carry genes responsible for genetic transformation of plants. See, for example, Kado, Crit. Rev. Plant Sci., 10:1-32 (1991). Descriptions of the  Agrobacterium  vector systems and methods for  Agrobacterium -mediated gene transfer are provided in Gruber et al., supra; and Moloney, et al, Plant Cell Reports, 8: 238-242 (1989). 
     Transgenic  Arabidopsis  plants can be produced easily by the method of dipping flowering plants into an  Agrobacterium  culture, based on the method of Andrew Bent in, Clough S J and Bent A F, 1998. Floral dipping: a simplified method for  Agrobacterium -mediated transformation of  Arabidopsis thaliana . Wild type plants are grown until the plant has both developing flowers and open flowers. The plant are inverted for 1 minute into a solution of  Agrobacterium  culture carrying the appropriate gene construct. Plants are then left horizontal in a tray and kept covered for two days to maintain humidity and then righted and bagged to continue growth and seed development. Mature seed is bulk harvested. 
     Direct Gene Transfer 
     A generally applicable method of plant transformation is microprojectile-mediated transformation, where DNA is carried on the surface of microprojectiles measuring about 1 to 4 mum. The expression vector is introduced into plant tissues with a biolistic device that accelerates the microprojectiles to speeds of 300 to 600 m/s which is sufficient to penetrate the plant cell walls and membranes. (Sanford, et al., Part. Sci. Technol., 5: 27-37 (1987); Sanford, Trends Biotech, 6: 299-302 (1988); Sanford, Physiol. Plant, 79: 206-209 (1990); Klein, et al., Biotechnology, 10: 286-291 (1992)). 
     Another method for physical delivery of DNA to plants is sonication of target cells as described in Zang, et al., BioTechnology, 9: 996-996 (1991). Alternatively, liposome or spheroplast fusions have been used to introduce expression vectors into plants. See, for example, Deshayes, et al., EMBO J., 4: 2731-2737 (1985); and Christou, et al., Proc. Nat&#39;l. Acad. Sci. (USA), 84: 3962-3966 (1987). Direct uptake of DNA into protoplasts using CaCl.sub.2 precipitation, polyvinyl alcohol or poly-L-ornithine have also been reported. See, for example, Hain, et al., Mol. Gen. Genet., 199: 161 (1985); and Draper, et al., Plant Cell Physiol., 23: 451-458 (1982). 
     Electroporation of protoplasts and whole cells and tissues has also been described. See, for example, Donn, et al., (1990) In: Abstracts of the VIIth Intl. Congress on Plant Cell and Tissue Culture IAPTC, A2-38, page 53; D&#39;Halluin et al., Plant Cell, 4: 1495-1505 (1992); and Spencer et al., Plant Mol. Biol., 24: 51-61 (1994). 
     Particle Wounding/ Agrobacterium  Delivery 
     Another useful basic transformation protocol involves a combination of wounding by particle bombardment, followed by use of  Agrobacterium  for DNA delivery, as described by Bidney, et al., Plant Mol. Biol., 18: 301-31 (1992). Useful plasmids for plant transformation include Bin 19. See Bevan, Nucleic Acids Research, 12: 8711-8721 (1984), and hereby incorporated by reference. 
     In general, the intact meristem transformation method involves imbibing seed for 24 hours in the dark, removing the cotyledons and root radical, followed by culturing of the meristem explants. Twenty-four hours later, the primary leaves are removed to expose the apical meristem. The explants are placed apical dome side up and bombarded, e.g., twice with particles, followed by co-cultivation with  Agrobacterium . To start the co-cultivation for intact meristems,  Agrobacterium  is placed on the meristem. After about a 3-day co-cultivation period the meristems are transferred to culture medium with cefotaxime plus kanamycin for the NPTII selection. 
     The split meristem method involves imbibing seed, breaking of the cotyledons to produce a clean fracture at the plane of the embryonic axis, excising the root tip and then bisecting the explants longitudinally between the primordial leaves. The two halves are placed cut surface up on the medium then bombarded twice with particles, followed by co-cultivation with  Agrobacterium . For split meristems, after bombardment, the meristems are placed in an  Agrobacterium  suspension for 30 minutes. They are then removed from the suspension onto solid culture medium for three day co-cultivation. After this period, the meristems are transferred to fresh medium with cefotaxime plus kanamycin for selection. 
     Transfer by Plant Breeding 
     Alternatively, once a single transformed plant has been obtained by the foregoing recombinant DNA method, conventional plant breeding methods can be used to transfer the gene and associated regulatory sequences via crossing and backcrossing. Such intermediate methods will comprise the further steps of: (1) sexually crossing the transgenic plant with a plant from a second taxon; (2) recovering reproductive material from the progeny of the cross; and (3) growing transgenic plants from the reproductive material. Where desirable or necessary, the agronomic characteristics of the second taxon can be substantially preserved by expanding this method to include the further steps of repetitively: (1) backcrossing the transgenic progeny with non-transgenic plants from the second taxon; and (2) selecting for expression of an associated marker gene among the progeny of the backcross, until the desired percentage of the characteristics of the second taxon are present in the progeny along with the gene or genes imparting marker gene trait. 
     By the term “taxon” herein is meant a unit of botanical classification. It thus includes, genus, species, cultivars, varieties, variants and other minor taxonomic groups which lack a consistent nomenclature. 
     Regeneration of Transformants 
     The development or regeneration of plants from either single plant protoplasts or various explants is well known in the art (Weissbach and Weissbach, 1988). This regeneration and growth process typically includes the steps of selection of transformed cells, culturing those individualized cells through the usual stages of embryonic development through the rooted plantlet stage. Transgenic embryos and seeds are similarly regenerated. The resulting transgenic rooted shoots are thereafter planted in an appropriate plant growth medium such as soil. 
     The development or regeneration of plants containing the foreign, exogenous gene that encodes a polypeptide of interest introduced by  Agrobacterium  from leaf explants can be achieved by methods well known in the art such as described (Horsch et al., 1985). In this procedure, transformants are cultured in the presence of a selection agent and in a medium that induces the regeneration of shoots in the plant strain being transformed as described (Fraley et al., 1983). In particular, U.S. Pat. No. 5,349,124 (specification incorporated herein by reference) details the creation of genetically transformed lettuce cells and plants resulting therefrom which express hybrid crystal proteins conferring insecticidal activity against Lepidopteran larvae to such plants. 
     This procedure typically produces shoots within two to four months and those shoots are then transferred to an appropriate root-inducing medium containing the selective agent and an antibiotic to prevent bacterial growth. Shoots that rooted in the presence of the selective agent to form plantlets are then transplanted to soil or other media to allow the production of roots. These procedures vary depending upon the particular plant strain employed, such variations being well known in the art. 
     Preferably, the regenerated plants are self-pollinated to provide homozygous transgenic plants, or pollen obtained from the regenerated plants is crossed to seed-grown plants of agronomically important, preferably inbred lines. Conversely, pollen from plants of those important lines is used to pollinate regenerated plants. A transgenic plant of the present invention containing a desired polypeptide is cultivated using methods well known to one skilled in the art. 
     A preferred transgenic plant is an independent segregant and can transmit the gene and its activity to its progeny. A more preferred transgenic plant is homozygous for the gene, and transmits that gene to all of its offspring on sexual mating. Seed from a transgenic plant may be grown in the field or greenhouse, and resulting sexually mature transgenic plants are self-pollinated to generate true breeding plants. The progeny from these plants become true breeding lines that are evaluated for increased expression of the transgene. 
     EMBODIMENTS 
     The constructs and methods of this invention have numerous applications of commercial value, especially in the prevention of desiccation of plant tissues under periods of water stress. Genetic manipulation of crop plants incorporating inhibitors of Ftase or inactivation of the gene encoding endogenous plant Ftase would allow such plants to withstand transitory environmental stress and can broaden the environments where these plants can be grown. Thus, improving tolerance of crop plants to cold, salt and drought stress, can improve the yield of the plants under such adverse conditions. 
     The technology described herein can also be used to alter harvesting time and harvest quality of plants. For example, overexpression of Ftase could lead to faster drying times of crops, such as corn and other grasses. Drying corn involves the use of large amounts of propane gas. Drying times of crops such as hay, which dry naturally in the fields, could be shortened, making it less likely that rain would deteriorate the crop. 
     In addition, inhibition of farnesylation in plants can also be used to control the senescence program of the plants so that leaves can be maintained in a green state longer and fruits can be kept immature. For example, if an antisense construct of ERA1 or CaaX box inhibitor protein construct was placed under the control of a senescence-induced promoter, the plant would induce an inhibitor of farnesylation as the senescence program was initiated, which would in turn inhibit senescence. The result would be a plant which remains green or fruits which remain immature. Thus, the plant could be kept producing a product, such as a vegetative part, flower or fruit much longer. Thus, horticulturalists could produce plants which stayed green and continued to grow even though a wild-type plant of the same variety would senesce under the same conditions. Cut flowers could be maintained longer. Or a fruit could be kept immature, an important product for the vegetable industry where produce lifetime to market is extremely important. 
     Further, the inhibition of Ftase in fruits and vegetables can reduce wilting. Thus, wilting of produce during transport and shipping could be reduced. Fruits and vegetables on the grocery shelf would also require less misting to keep them fresh and flavorful, and there would be less need to wax produce such as cucumber, apples and oranges to keep them from drying out. 
     Less watering would also mean that fungal and bacterial attacks on the crops, or fruits and vegetables would be reduced. For example, plant diseases in the field which result from splashing of plant pathogens from the soil to the plant leaves and fruits could be inhibited. 
     In the field of horticulture, many drought-resistant varieties could be produced for landscaping and for use as ornamental house plants. Especially valuable would be varieties of plants which are used for potting, as ornamentals inside or outside homes and offices, and which can survive infrequent water. This would be a considerable boon for gardeners, especially during the droughty summer months where forgotten plants dry out quickly in the sun. Further, plants grown under trees and in other shady areas often experience drought conditions and limited light. The technology provided herein can provide plant varieties which can better survive under these conditions. 
     In a further embodiment, horticulturalists could find many uses for plants wherein lateral branching and/or flower numbers can be regulated with light/dark cycles. Examples of plants in which longer, unbranched stems would confer marketable advantage include roses, carnations, lilies, and the like. The ability to increase the number of flowers or florets on the plant is also a highly valuable asset. These traits could also be useful for many agricultural crops in that yields can be increased in a manner which also made harvesting of the crop easier. 
     Another benefit of the constructs and methods provided herein is that the ERA1 promoter is active in the guard cells of leaves. A portion of the ERA1 gene promoter can be fused to antisense nucleic acid to the ERA1 gene so Ftase activity is diminished only in the guard cells. 
     A further embodiment is the use of the drought-resistant trait as a selectable marker of transformation in plants, plant cells and plant tissues. One method of detecting transformation in plants consists of: (a) incorporating a nucleic acid construct comprising a promoter operably-linked to nucleic acid comprising antisense to SEQ ID NO: 1 or nucleic acid comprising a functional equivalent or fragment thereof of the antisense; (b) inserting the nucleic acid construct into a plant, plant cell or plant tissue; (c) growing the plant, or regenerating a plant from the plant cell or plant tissue until stomates are formed; and (d) placing the plant or regenerated plant under conditions wherein the plant is drought stressed, wherein survival of the plant under drought conditions compared to untransformed plants is indicative of transformation. Thus, this technology can be used as a selectable genetic marker, i.e., a visual marker especially when combined with plant selection and transformation schemes. 
     In addition, without resorting to stressing a transgenic plant, the branching and/or flowering habit of plants with loss of Ftase function differs substantially from that of wild-type plants and can be used as a marker for successful transformation. This method would be especially useful where in planta transformation techniques have been applied. Under diurnal light conditions, shoots of transgenic plants will demonstrate less lateral branching than that of untransformed shoots, thus indicating effective loss of Ftase activity without the use of selective antibiotic markers. 
     EXEMPLIFICATION 
     Example 1 
     Mutagenesis Conditions 
       Arabidopsis  plants used in this study were grown under continuous light in soil- or agar-containing petri plates as described elsewhere (Haughn and Somerville 1986). Two distinct wild-types of  Arabidopsis  were used: Meyerowitz&#39;s Colombia (MCol) (Lelhe Seeds, Dripping Springs, Tex.) and Wassilewskija (Ws) (ABRC, Ohio State University). T-DNA mutagenized seeds were screened and mutants were isolated in the Wassilewskija background. These were obtained from the Ohio State  Arabidopsis  seed stock collection (ABRC stock numbers CS2606-2654). The T-DNA seed collection was comprised of 49 pools of 1200 fourth generation (T4) offspring derived from 100 mutagenized parents. A mutagenized parent was obtained by incubating wild-type (Ti) seeds overnight in a saturating  Agrobacterium  culture containing a T-DNA plasmid carrying a gene conferring kanamycin resistance. The seeds were then washed in water and planted into pots. T2 generation seed were obtained from each plant and tested for kanamycin resistance. Kanamycin-resistant plants were advanced to the T3 generation. T4 generation plants were given to the stock center. Each pool was screened separately. 
     Fast neutron-irradiated seeds were screened and mutants were isolated in Meyerowitz&#39;s Columbia background. Mutagenized wild-type seeds (N1) were irradiated with 60 Gy of fast neutrons and grown to the next generation. The N2 seeds were obtained as pools of approximately 11,000 seeds generated from 1387 N1 parents. Ten of these pools were screened separately for ABA supersensitive mutations. In the initial screen, all seeds had been stored at 4° C. and were plated without imbibing. For all subsequent screens, seeds were imbibed at 4° C. for one week on 0.3 μM ABA and scored for cotyledon emergence after 5-7 days at 22° C. in the light. 
     Example 2 
     Genetic Analysis 
     Mutant lines were backcrossed to wild type three times. T-DNA mutations were backcrossed to Ws and fast neutron mutants to MCol. Segregation of the era phenotype was followed by plating F2 seeds on both 0.3 μM ABA and imbibing four days at 4° C. Following imbibition, plates were transferred to room temperature in the light. Germination was measured as the presence or absence of expanded cotyledons in seedlings one week after imbibition. Double mutants were constructed by crossing lines homozygous for each mutation following segregation and identifying lines that carried one of the mutant phenotypes. The abi3 allele used in this study is abi3-6 (Nambara et al., 1994) and the abi1 allele is abi1-1 (Koomneef et al, 1982). The era1-2 allele was used as the era parent. Segregation analysis suggested era1 partially suppressed the insensitivity of abi1 to ABA, so F2 plants were first screened for insensitivity to 3 mM ABA, and F3 seed from these plants were scored for sensitivity to 0.3 μM ABA. Putative era1 abi1 double mutants were progeny-tested in the F4 generation and verified by DNA polymorphism analysis for both Era 1 and Abi1. For era1 abi3 double mutants, F2 seeds were screened for insensitivity to 3 μM ABA, and mature plants were scored for protruding carpels and immature green seeds (Nambara et al., 1994). Putative double mutant lines were also verified by DNA polymorphism analysis for both Era1 and Abi3. 
     Example 3 
     DNA and RNA Analysis 
     The methods employed for DNA (Dellaporta et al., 1983) and RNA (Verwoerd et al., 1989) extractions were as described elsewhere. High stringency Southern blots were carried out at 65° C. according to standard protocols described elsewhere (Sambrook et al., 1989). All genomic and cDNA library screening was done on Gelman BioTrace NT membranes according to the manufacturer&#39;s specifications (Gelman Sciences). To clone insertion junctions between T-DNA and genomic DNA in the era1-1 mutant (isolated from T12W DNA) a library of T12W DNA was made in γ-ZAPII (Stratagene). Genomic Southern blots of T12W DNA digested with restriction endonuclease EcoR I and probed with right border (R B ) T-DNA produced three bands (13.0 Kb, 7.0 Kb and 8.0 Kb). Subsequent analysis with additional restriction enzymes verified that the 7.0 and 8.0 Kb bands contained the insertion junctions between T-DNA and flanking plant DNA. These fragments were cloned by digesting genomic DNA with EcoR I, fractionating the digested DNA using a Prep Cell (Pharmacia), and identifying the fractions containing the 7.0 and 8.0 Kb by Southern blot analysis using the R B  as a probe. Pooled fractions containing both the 7.0 and 8.0 Kb fragments were then ligated to the γ-ZAPII vector arms according to the manufacturer&#39;s instructions (Stratagene). A library containing approximately 40,000 individual recombinant bacteriophage was screened. Five positive plaques were identified and excised plasmid forms of the cloned inserts were isolated according to the manufacturer&#39;s instructions (Stratagene). Two plasmids which hybridized to the RB probe were designated pL4B and pL7 and selected for further characterization. A 2.3 kB EcoR I-BamH I restriction fragment from clone pL4B was subcloned into the plasmid pBluescript and designated pSC10. A 1.3 Kb Hind III-BamH I restriction fragment from clone pL7 was also subcloned into pBluescript and designated pSC11. Each of these plasmids contains approximately 1.2 Kb of T-DNA attached to the flanking plant genomic DNA. pSC10 was used as a probe to screen an  Arabidopsis  cDNA library called PRL2 λ-ZipLox (ABRC, Stock CD4-7). This screen identified five positive cDNAs, and the longest cDNA insert, clone pZL23, was used to screen an additional 200,000 recombinant PRL2 phage. Subsequently a longer cDNA insert, clone pZL51, which contained an insert of 1.35 Kb, was isolated. Both cDNA clones pZL23 and pZL51 were sequenced and used to screen 30,000 γ-ZAPII plaques made from wild-type Columbia genomic DNA partially digested with EcoR I. Construction of this library was as described above except the digested DNA was not size-fractionated. This screen identified four positive clones. The inserts were excised and excised plasmid forms of the cloned inserts were isolated according to the manufacturer&#39;s instructions. A 6 Kb region encompassing the entire pZL51 clone was completely sequenced. This genomic insert and a 14 Kb genomic insert isolated by screening a λ-FIX genomic library from  Lansberg erecta  via similar methods (ABRC Stock CD4-8) were used as probes to analyze deletion size in the fast neutron mutants era1-2 and era1-3. 
     Example 4 
     Protein Farnesyl Transferase Assay 
     Farnesyl transferase (Ftase) assays were performed using Ftase from cell-free extracts of wild-type and mutant plants and synthetic heptapeptides as substrate for the reaction. Peptides were purchased from Genemed Biotechnologies, Inc. The peptide sequences used were based on the data of Randall et al. (1993): GGCCAIM (-CAIM) and GGCCAIL (-CAIL). Solutions of peptides were prepared in 100% dimethyl sulfoxide (DMSO) containing 10 mM dithiotreitol (DTT) and diluted in 10 mM DTT without DMSO. The cell-free extracts contained soluble protein isolated from the buds of three week old plants, either wild-type or mutant strains. First 1 g of fresh buds was collected and homogenized in a buffer containing 50 mM Hepes (pH 7.5), 1 mM MgCl 2 , 1 mM EGTA, 5 mM DTT, 2 μg/ml leupeptin, 2 μg/ml aprotinin, and 1 mM PMSF. Next, cellular debris and membranes were removed by centrifugation at 4° C. at 10,000×g for 10 minutes and 100,000×g for 30 minutes. Following the second centrifugation, the supernatant was decanted and total soluble protein was quantified by the method of Bradford (1976). Soluble protein extracts were incubated at 30° C. with a peptide substrate and radio-labeled  3 H-farnesyl pyrophosphate (FPP) (Amersham) for 40 minutes. Each reaction mixture contained the following components in a final volume of 25 μl: 50 mM Hepes (pH 7.5), 5 mM MgCl 2 , 5 mM DTT, 50 μM peptide, 0.5 μM [ 3 H]FPP, and 100 μg of soluble protein extract. One control reaction contained soluble protein extracts that had been boiled for 5 minutes to irreversibly denature all protein. Reactions were terminated by adding EDTA to a final concentration of 50 mM and then spotted onto Silica Gel 60 thin-layer chromatography (TLC) plates (Millipore). TLC plates were developed with n-propanol and water (7:3 v/v) for 4-5 hours. The plates were dried, sprayed with En 3 Hance (New England Nuclear), and exposed to Kodak X-OMAT AR film at −70° C. for 4 days. 
     Example 5 
     ERA1-β-glucuronidase Gene Constructs and Transgenic Plants 
     ERA1-β-glucuronidase (ERA1-GUS) fusion constructs were made by inserting a 5 Kb EcoR I-Hind III genomic fragment of the ERA1 promoter into a promoterless GUS T-DNA plasmid pBT121 containing a gene conferring resistance to the antibiotic ampicillin. This construct was then transformed into  Agrobacterium  strain LB4404. The  Agrobacterium  was grown to a density of 0.8 O.D. units (measured at 595 nm). The cells were then washed extensively in water, resuspended in sterile 10% glycerol and purified plasmid DNA encoding the ERA1-GUS fusion construct was added. Finally, the mixture of cells and DNA was pulsed in an electroporator at 200 Ohms 25 RF, 2.5 kvolts. Cells were then plated on Luria Broth agar plates containing 100 μg/ml ampicillin and grown for 2 days at 28° C. Ampicillin-resistant transformants were cultured and plasmid DNA isolated from the cultures by standard techniques was used in subsequent plant transformation experiments. 
     Transgenic plants were made by vacuum infiltrating plants with a saturated  Agrobacterium  culture grown to a density of 0.8 O.D. units as measured at 595 nm. Wild-type plants were grown under standard laboratory conditions (at 25° C., 150 μm −2  sec −1 , humidity, constant light) until they produced their first bolts at approximately 5 weeks. Next, plant stems were removed and the plants were submerged in a solution of  Agrobacterium  and placed under a 20 mBar vacuum for 5 minutes. After the vacuum was broken, the plants were transferred to soil and allowed to recover under standard laboratory conditions as described above. After two months, the plants produced new flowers and seed which was harvested and allowed to dry for 2 weeks. Seed from individual plants were planted onto Murashige and Skoog (MS) minimal medium plates containing 50 μg/ml kanamycin. Green kanamycin-resistant plantlets were identified and transferred to soil after 2 weeks and allowed to grow for seed. These seeds were germinated and the seedlings were tested for GUS activity using the fluorescent GUS substrate Imagene Green (Molecular Probes, Eugene, Oreg.). GUS activity was assayed by suspending seedlings in GUS buffer (50 mM Sodium phosphate, pH 7.0, 10 mM EDTA, 0.1% Triton X-100, 0.1% Sodium sarcosyl, 4 mM Imagene Green) for 2-4 hours in the dark at room temperature. Seedlings were viewed under a microscope at 25× magnification using blue light to generate a positive fluorescent signal. When this mixture is treated with blue light, GUS activity will produce yellow light in a background of red auto-fluorescence generated by red chlorophyll. 
     Example 6 
     Drought Experiments 
     Six wild-type and six era1-2 seedlings were grown for four weeks in constant light with constant watering (25° C., 150 μm −2  sec −1 , 70% humidity, constant light). The plant and pot were weighed and the pots were then covered with aluminum foil to retard soil evaporation. At this time, plants were no longer watered and each pot was weighed daily. At the end of the experiment plants were removed from the pots, which were allowed to dry for another two weeks, when they were weighed to determine the weight of the dry soil and pot. This weight was subtracted from each sample. 
     Example 7 
     Age-Related Changes in Detached Leaves 
     The chlorophyll content in adult rosette leaves in wild-type Columbia and era1-2 mutants were compared after detachment from plants. The plants were grown under constant light and temperature (150 μE/m 2 ·sec, 22° C.) to a similar developmental age of 3 weeks after germination. At this time, the fifth leaves of several plants which had emerged after germination were removed and placed on petri plates containing 0.8% agar with minimal salts. The plates were sealed and placed at 22° C. under constant light (50 μE/m 2 ·sec) for 12 days. Photographs were taken and color comparisons made at 0, 3, 6, 9, and 12 days. 
     Example 8 
     Determination of Transcript Levels for Selected Genes in Aging Leaves 
     Mutant (era1-2) and wild-type plants were grown under constant light and temperature (150 μE/m 2 ·sec, 22° C.) to a similar developmental age of 4 weeks after germination. At that time, the fifth rosette leaf which had emerged following germination was removed from all plants. These leaves were assayed for expression levels of three genes:  Arabidopsis  chlorophyll binding protein (CAB) and senescence-activated genes 12 and 13 (SAG12 and SAG13). mRNA transcript levels were assayed by Northern blot analysis at 0, 4, 8 days after the plants bolted. The CAB gene encodes the  Arabidopsis  chlorophyll binding protein which is involved in capturing light for photosynthesis. It is required for the green color of the leaf and is a good marker of chlorophyll turnover in the plant. CAB in wild-type plants shows transcript level reduction upon induction of senescence. No transcript level reduction was observed in aging leaves of era1-2 mutants. SAG12 and SAG13 are  Arabidopsis  genes cloned by differential expression during senescence (SAG stands for senescence activated gene). Transcription of both genes is induced during the onset of senescence in wild-type  Arabidopsis  plants. These genes were not induced under the same developmental conditions in the era1-2 mutants. 
     Example 9 
     Cloning of  Arabidopsis thaliana  FTA and Construction of Transformation Vector 
     The  Arabidopsis thaliana  FTA sequence was obtained by RT-PCR from total RNA isolated from leaf tissue using primers corresponding to SEQ ID NO: 17 and SEQ ID NO:18. The resulting fragment was digested with BamHI and SmaI and cloned into the plasmid pCR2.1 The Clonetech vector pBI121 was used as the backbone for the antisense construct. The GUS gene was removed by BamHI and Eco1 CR1 digestion and replaced with the FTA insert that was cut from pCR2.1-FTA using SmaI and BamHI and ligated into the vector SEQ ID NO: 10. 
     
       
         
           
               
               
             
               
                 TABLE 1 
               
               
                   
               
             
            
               
                 SEQ ID NO: 17: 
                 5′-AAAGGATCCTCAAATTGCTGCCACTGTAAT-3′ 
               
               
                   
               
               
                 SEQ ID NO: 18: 
                 5′-AAACCCGGGATGAATTTCGACGAGAACGTG-3′ 
               
               
                   
               
            
           
         
       
     
     Example 10 
     Cloning of Non-Full Length  Brassica napus  FTA and FTB Nucleic Acid Sequences 
     RNA was isolated from leaf and root tissue using the Qiagen RNeasy kit. RT-PCR was performed by known techniques using the primers shown in Table 2. The FTA sequence was obtained using the primer pair SEQ ID NO:25 and SEQ ID NO:26. The FTB sequence was obtained using the primer pair SEQ ID NO:27 and SEQ ID NO:28. 
     
       
         
           
               
               
             
               
                 TABLE 2 
               
               
                   
               
             
            
               
                 SEQ ID NO: 25: 
                 5′-GGATCCATGGATTACTTCCGTGCGATTTAC 
               
               
                   
                 TTCTCC-3′ 
               
               
                   
               
               
                 SEQ ID NO: 26: 
                 5′-AAAAAGCTTCCATGCCCAATAGTTAGCTCT 
               
               
                   
                 TATTGGATC-3′ 
               
               
                   
               
               
                 SEQ ID NO: 27: 
                 5′-AAAAAGCTTTGGCTTTGTTACTGGATTCTTCAT 
               
               
                   
                 TCAAT-3′ 
               
               
                   
               
               
                 SEQ ID NO: 28: 
                 5′-AAATCTAGAAGCTTCATAATACCGATCC 
               
               
                   
                 AAGACAATGTT-3′ 
               
               
                   
               
            
           
         
       
     
     PCR products were separated from the RT-PCR reaction mixture using the Qiagen PCR column spin kit and ligated into the cloning vector pBluescript KS+. The vector was digested with EcoRV and treated with Taq polymerase in the presence of dTTP to produce a 3′ overhang for ligation with the PCR products. The ligation products were transformed into  E. coli  DH5α cells, positive colonies were selected and the resulting inserts sequenced. 
     Example 11 
     Cloning of Non-Full Length FTA and FTB Nucleic Acid Sequences from  Glycine max  and  Zea maize    
     RNA was isolated from leaf and root tissue using the Qiagen RNeasy kit. RT-PCR was performed by known techniques using the primers shown in Table 3. The  Glycine max  FTA sequence was obtained using the primer pair SEQ ID NO:29 and SEQ ID NO:30. The  Glycine max  FTB sequence was obtained using the primer pair SEQ ID NO:31 and SEQ ID NO:32. The  Zea maize  FTB sequence was obtained using the primer pair SEQ ID NO:33 and SEQ ID NO:34 
     
       
         
           
               
               
             
               
                 TABLE 3 
               
               
                   
               
             
            
               
                 SEQ ID NO: 29: 
                 5′-AAAGGATCCATGGAATCTGGGTCTAGCGA-3′ 
               
               
                   
               
               
                 SEQ ID NO: 30: 
                 5′-AAATCTAGAAGGAAGTCTGCTCTTGCGC-3′ 
               
               
                   
               
               
                 SEQ ID NO: 31: 
                 5′-AAATCTAGAGCCACCATTCCTCGCAACG-3′ 
               
               
                   
               
               
                 SEQ ID NO: 32: 
                 5′-AAAGAGCTCGTGGTGGTGAATCTGGGTGC-3′ 
               
               
                   
               
               
                 SEQ ID NO: 33: 
                 5′-GGCGGATCCCGACCTACCGAGG-3′ 
               
               
                   
               
               
                 SEQ ID NO: 34: 
                 5′-AAAGAGCTCGTGGATGGATTGGCTCCAGC-3′ 
               
               
                   
               
            
           
         
       
     
     PCR products were separated from the RT-PCR reaction mixture using the Qiagen PCR column spin kit and ligated into the cloning vector pBluescript KS +. The vector was digested with EcoRV and treated with Taq polymerase in the presence of dTTP to produce a 3′ overhang for ligation with the PCR products. The ligation products were transformed into  E. coli  DH5α cells, positive colonies were selected and the resulting inserts sequenced. 
     Example 12 
     Sequence Analysis 
       Arabidopsis thaliana  FTA 
     A disclosed nucleic acid of 999 nucleotides (also referred to as FT1) is shown in Table 4A. The primers used in the PCR are depicted in bold. 
     
       
         
           
               
             
               
                 TABLE 4A 
               
               
                   
               
               
                 FT1 Nucleotide Sequence (SEQ ID NO: 7). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                   Aaacccgggatgaatttcgacgagaccgtg ccactgagccaacgattgga 
               
               
                   
               
               
                 gtggtcagacgtggtcccattgactcaggacgatggtccgaatccagtgg 
               
               
                   
               
               
                 tgccaattgcctacaaggaagagttccgcgagactatggattacttccgt 
               
               
                   
               
               
                 gcgatttacttttccgacgagcgatctcctcgcgcactacgactcacgga 
               
               
                   
               
               
                 agaaaccctcctcttaaactccggcaactacacagtgtggcatttcaggc 
               
               
                   
               
               
                 gcctagtactcgaggcccttaatcacgacttgtttgaagaactcgagttc 
               
               
                   
               
               
                 atcgaacgcattgctgaggataactctaagaactaccaactgtggcatca 
               
               
                   
               
               
                 tcggcgatgggttgcagagaaactgggtcctgatgttgcagggagagaac 
               
               
                   
               
               
                 ttgaatttacccgtagagtactttcacttgatgccaaacattatcatgct 
               
               
                   
               
               
                 tggtcacataggcagtggacactacgggcattaggaggatgggaagatga 
               
               
                   
               
               
                 gctcgattactgtcacgagctccttgaagctgacgtctttaacaattccg 
               
               
                   
               
               
                 cctggaatcagaggtattatgtcatcacccaatctcctttgttgggaggc 
               
               
                   
               
               
                 ctagaagccatgagagaatctgaagtaagctacacaatcaaagccatttt 
               
               
                   
               
               
                 aaccaatcctgcaaacgagagctcatggcgatacctaaaagcgctttaca 
               
               
                   
               
               
                 aagacgacaaagaatcctggattagtgatccaagtgtttcctcagtctgt 
               
               
                   
               
               
                 ttgaatgttctatcccgcacagattgcttccatggattcgctctgagcac 
               
               
                   
               
               
                 ccttttggatcttctatgtgatggactgagaccaaccaacgagcataaag 
               
               
                   
               
               
                 actcagtgagagctctagctaatgaagaaccagagactaacttggccaat 
               
               
                   
               
               
                 ttggtgtgtactattcttggtcgtgtagatcctataagagctaactattg 
               
               
                   
               
               
                 ggcatggaggaagagcaagatt acagtggcagcaatttgaggatccttt   
               
               
                   
               
            
           
         
       
     
     A disclosed FT1 polypeptide (SEQ ID NO: 11) encoded by SEQ ID NO:7 has 326 amino acid residues and is presented in Table 4B using the one-letter amino acid code. 
     
       
         
           
               
             
               
                 TABLE 4B 
               
               
                   
               
               
                 Encoded FT1 protein sequence (SEQ ID NO: 11). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 MNFDETVPLSQRLEWSDVVPLTQDDGPNPVVPIAYKEEFRETMDYFRAIY 
               
               
                   
               
               
                 FSDERSPRALRLTEETLLLNSGNYTVWHFRRLVLEALNHDLFEELEFIER 
               
               
                   
               
               
                 IAEDNSKNYQLWHHRRWVAEKLGPDVAGRELEFTRRVLSLDAKHYHAWSH 
               
               
                   
               
               
                 RQWTLRALGGWEDELDYCHELLEADVFNNSAWNQRYYVITQSPLLGGLEA 
               
               
                   
               
               
                 MRESEVSYTIKAILTNPANESSWRYLKALYKDDKESWISDPSVSSVCLNV 
               
               
                   
               
               
                 LSRTDCFHGFALSTLLDLLCDGLRPTNEHKDSVRALANEEPETNLANLVC 
               
               
                   
               
               
                 TILGRVDPIRANYWAWRKSKITVAAI 
               
               
                   
               
            
           
         
       
     
     Due to the nature of the cloning strategy the sequence presented does not contain any 5′ or 3′ non-translated sequence. Using the sequences disclosed herein as hybridization probes, one is able to screen and isolate full length sequences from cDNA or genomic libraries or use the rapid amplification of cDNA ends (RACE) technology or other such PCR techniques. The percent identity of the  Arabidopsis thaliana  nucleotide sequence and its encoded amino acid sequence to that of published sequences is shown in  FIG. 17 . 
     The present invention also includes a nucleic acid sequence complementary to the  Arabidopsis thaliana  farnesyl transferase alpha subunit of SEQ ID NO:7. The disclosed complementary sequence is shown as SEQ ID NO:8. The nucleic acid sequence of SEQ ID NO:9 shows the nucleic acid sequence of SEQ ID NO:8 that has been prepared for ligation into an expression vector. 
                        SEQ ID NO: 8                           aaaggatcctcaaattgctgccactgtaat cttgctcttcctccatgcccaatagttagctctt                   ataggatctacacgaccaagaatagtacacaccaaattggccaagttagtctctggttcttcat               tagctagagctctcactgagtctttatgctcgttggttggtctcagtccatcacatagaagatc               caaaagggtgctcagagcgaatccatggaagcaatctgtgcgggatagaacattcaaacagact               gaggaaacacttggatcactaatccaggattctttgtcgtctttgtaaagcgcttttaggtatc               gccatgagctctcgtttgcaggattggttaaaatggctttgattgtgtagcttacttcagattc               tctcatggcttctaggcctcccaacaaaggagattgggtgatgacataatacctctgattccag               gcggaattgttaaagacgtcagcttcaaggagctcgtgacagtaatcgagctcatcttcccatc               ctcctaatgcccgtagtgtccactgcctatgtgaccaagcatgataatgtttggcatcaagtga               aagtactctacgggtaaattcaagttctctccctgcaacatcaggacccagtttctctgcaacc               catcgccgatgatgccacagttggtagttcttagagttatcctcagcaatgcgttcgatgaact               cgagttcttcaaacaagtcgtgattaagggcctcgagtactaggcgcctgaaatgccacactgt               gtagttgccggagtttaagaggagggtttcttccgtgagtcgtagtgcgcgaggagatcgctcg               tcggaaaagtaaatcgcacggaagtaatccatagtctcgcggaactcttccttgtaggcaattg               gcaccactggattcggaccatcgtcctgagtcaatgggaccacgtctgaccactccaatcgttg               gctcagtgg cacggtctcgtcgaaattcatcccgggttt                               SEQ ID NO: 9                             tcaaattgctgccactgtaat cttgctcttcctccatgcccaatagttagctcttatag                   gatctacacgaccaagaatagtacacaccaaattggccaagttagtctctggttcttcattagc               tagagctctcactgagtctttatgctcgttggttggtctcagtccatcacatagaagatccaaa               agggtgctcagagcgaatccatggaagcaatctgtgcgggatagaacattcaaacagactgagg               aaacacttggatcactaatccaggattctttgtcgtctttgtaaagcgcttttaggtatcgcca               tgagctctcgtttgcaggattggttaaaatggctttgattgtgtagcttacttcagattctctc               atggcttctaggcctcccaacaaaggagattgggtgatgacataatacctctgattccaggcgg               aattgttaaagacgtcagcttcaaggagctcgtgacagtaatcgagctcatcttcccatcctcc               taatgcccgtagtgtccactgcctatgtgaccaagcatgataatgtttggcatcaagtgaaagt               actctacgggtaaattcaagttctctccctgcaacatcaggacccagtttctctgcaacccatc               gccgatgatgccacagttggtagttcttagagttatcctcagcaatgcgttcgatgaactcgag               ttcttcaaacaagtcgtgattaagggcctcgagtactaggcgcctgaaatgccacactgtgtag               ttgccggagtttaagaggagggtttcttccgtgagtcgtagtgcgcgaggagatcgctcgtcgg               aaaagtaaatcgcacggaagtaatccatagtctcgcggaactcttccttgtaggcaattggcac               cactggattcggaccatcgtcctgagtcaatgggaccacgtctgaccactccaatcgttggctc               agtgg cacggtctcgtcgaaattcat                  Brassica napus  FTA
 
     A disclosed nucleic acid of 822 nucleotides (also referred to as FT2) is shown in Table 5A. 
     
       
         
           
               
             
               
                 TABLE 5A 
               
               
                   
               
               
                 FT2 Nucleotide Sequence (SEQ ID NO: 12). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 ATGGATTACTTCCGTGCGATTTACTTCTCCGACGAGCGTTCTGCTCGCGC 
               
               
                   
               
               
                 GCTGCGACTCACGGAAGAAGCTCTCCGCTTAAACTCGGGCAACTACACCG 
               
               
                   
               
               
                 TGTGGCACTTCGGGCGCTTAGTACTCGAGGAGCTTAATAACGACTTGTAT 
               
               
                   
               
               
                 GAAGAGCTCAAGTTCATCGAAAGCATTGCTGAGGATAACTCTAAGAACTA 
               
               
                   
               
               
                 CCAGTTGTGGCATCATCGACGATGGGTCGCAGAGAAACTGGGTCCTGATG 
               
               
                   
               
               
                 TTGCAGGAAAGGAACTTGAGTTTACTCGGAGGGTACTATCACTTGATGCC 
               
               
                   
               
               
                 AAGCATTATCATGCTTGGTCACATAGGCAGTGGGCGCTACAAGCATTAGG 
               
               
                   
               
               
                 AGGATGGGAAAATGAGCTTAACTACTGCCACGAGCTCCTTGAAGCTGACG 
               
               
                   
               
               
                 TCTTTAACAACTCTGCATGGAATCAGAGGTATTACGTTATAACTAGATCA 
               
               
                   
               
               
                 CCTTCGTTGGGAGGCCTAGAAGCCATGAGAGAATCTGAAGTAAGCTACAC 
               
               
                   
               
               
                 AGTCAAAGCCATTTTAGCAAATCCCGGGAACGAGAGCTCTTGGAGGTACC 
               
               
                   
               
               
                 TGAAAGCCCTTTACAAAGACGACACAGAGTCTTGGATTAGTGATCCAAGT 
               
               
                   
               
               
                 GTTTCCTCAGTCTGTTTGAAAGTTCTCTCACGCGCGGACTGCTTCCATGG 
               
               
                   
               
               
                 ATTCGCTCTGAGCACCCTTTTGGATCTTCTGTGCGATGGGTTGAGACCAA 
               
               
                   
               
               
                 CCAACGAGCATAGAGACTCGGTGAAAGCTCTAGCTAATGAAGAACCAGAG 
               
               
                   
               
               
                 ACTAACTTGGCCAATTTGGTGTGTACCATTCTGTGTCGTGTTGATCCAAT 
               
               
                   
               
               
                 AAGAGCTAACTATTGGGCATGG 
               
               
                   
               
            
           
         
       
     
     A disclosed FT2 polypeptide (SEQ ID NO: 13) encoded by SEQ ID NO: 12 has 274 amino acid residues and is presented in Table 5B using the one-letter amino acid code. 
     
       
         
           
               
             
               
                 TABLE 5B 
               
               
                   
               
               
                 Encoded FT2 protein sequence (SEQ ID NO: 13). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 MDYFRAIYFSDERSARALRLTEEALRLNSGNYTVWHFGRLVLEELNNDLY 
               
               
                   
               
               
                 EELKFIESIAEDNSKNYQLWHHRRWVAEKLGPDVAGLEKEFTRRVLSLDA 
               
               
                   
               
               
                 KHYHAWSHRQWALQALGGWENELNYCHELLEADVFNNSAWNQRYYVITRS 
               
               
                   
               
               
                 PSLGGLEAMRESEVSYTVKAILANPGNESSWRYLKALYKDDTESWISDPS 
               
               
                   
               
               
                 VSSVCLKVLSRADCFHGFALSTLLDLLCDGLRPTNEHRDSVKALANEEPE 
               
               
                   
               
               
                 TNLANLVCTILCRVDPIRANYWAWKL 
               
               
                   
               
            
           
         
       
     
     Due to the nature of the cloning strategy the sequence presented is not full length. Compared to the  Arabidopsis thaliana  sequence there are 42 amino acids missing from the amino terminus and 10 amino acids from the carboxy terminus. The percent identity of the  Brassica napus  nucleotide sequence and its encoded amino acid sequence to that of published sequences is shown in  FIG. 17 . 
     Using the sequences disclosed herein as hybridization probes, one is able to screen and isolate full length sequences from cDNA or genomic libraries or use the rapid amplification of cDNA ends (RACE) technology or other such PCR techniques. 
     The present invention also includes a nucleic acid sequence complementary to the  Brassica napsus  farnesyl transferase alpha subunit of SEQ ID NO:12. The disclosed complementary sequence is shown as SEQ ID NO:35. 
                    SEQ ID NO: 35                 CCATGCCCAATAGTTAGCTCTTATTGGATCAACACGACACAGAATGGTAC               ACACCAAATTGGCCAAGTTAGTCTCTGGTTCTTCATTAGCTAGAGCTTT               CACCGAGTCTCTATGCTCGTTGGTTGGTCTCAACCCATCGCACAGAAG               ATCCAAAAGGGTGCTCAGAGCGAATCCATGGAAGCAGTCCGCGCGTGAG               AGAACTTTCAAACAGACTGAGGAAACACTTGGATCACTAATCCAAGACT               CTGTGTCGTCTTTGTAAAGGGCTTTCAGGTACCTCCAAGAGCTCTCGTT               CCCGGGATTTGCTAAAATGGCTTTGACTGTGTAGCTTACTTCAGATTCT               CTCATGGCTTCTAGGCCTCCCAACGAAGGTGATCTAGTTATAACGTAAT               ACCTCTGATTCCATGCAGAGTTGTTAAAGACGTCAGCTTCAAGGAGCTC               GTGGCAGTAGTTAAGCTCATTTTCCCATCCTCCTAATGCTTGTAGCGCC               CACTGCCTATGTGACCAAGCATGATAATGCTTGGCATCAAGTGATAGTA               CCCTCCGAGTAAACTCAAGTTCCTTTCCTGCAACATCAGGACCCAGTTT               CTCTGCGACCCATCGTCGATGATGCCACAACTGGTAGTTCTTAGAGTTA               TCCTCAGCAATGCTTTCGATGAACTTGAGCTCTTCATACAAGTCGTTATT               AAGCTCCTCGAGTACTAAGCGCCCGAAGTGCCACACGGTGTAGTTGCC               CGAGTTTAAGCGGAGAGCTTCTTCCGTGAGTCGCAGCGCGCGAGCAGA               ACGCTCGTCGGAGAAGTAAATCGCACGGAAGTAATCCAT              Brassica napus  FTB
 
     A disclosed nucleic acid of 1110 nucleotides (also referred to as FT3) is shown in Table 6A. 
     
       
         
           
               
             
               
                 TABLE 6A 
               
               
                   
               
               
                 FT3 Nucleotide Sequence (SEQ ID NO: 14). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 TGGCTTTGTTACTGGATTCTTCATTCAATTGCTTTGCTTGGGGAGTCTGT 
               
               
                   
               
               
                 GGATGATGACTTAGAAAACAATGCAATCGATTTTCTTGGACGTTGCCAG 
               
               
                   
               
               
                 GGTTCTGATGGTGGATATGGTGGTGGTCCTGGCCAACTTCCACATCTTG 
               
               
                   
               
               
                 CAACAAGTTATGCTGCAGTGAATACACTTGTTACTTTAGGAGGTGAGAA 
               
               
                   
               
               
                 AGCCTTCTCTTCAATTAACAGAGAACAAATGGCTTGTTTCTTAAGACGAA 
               
               
                   
               
               
                 TGAAGGATACAAATGGAGGTTTCAGGATGCATAATATGGGAGAAATAGAT 
               
               
                   
               
               
                 GTGCGAGCGTGCTACACTGCGATTTTGATTGCAAGCATCCTGAACATTG 
               
               
                   
               
               
                 TGGATGATGAACTCACCCGCGGCTTAGGAGATTACATTTTGAGTTGCCA 
               
               
                   
               
               
                 ACTTATGAAGGTGGCATTGGAGGGGAACCTGGCTCCGAAGCTCATGGT 
               
               
                   
               
               
                 GGGTACACGTACTGTGGGTTGGCTACTATGATTTTAATCAATGAAGTCGA 
               
               
                   
               
               
                 CCGCTTGAATTTGGATTCGTTAATGAATTGGGTTGTACATCGACAAGGAG 
               
               
                   
               
               
                 TAGAAATGGGATTCCAAGGTAGGACGAACAAATTGGTCGACGGTTGCTA 
               
               
                   
               
               
                 CACGTTTTGGCAGGCAGCCCCCTGTGTTCTACTACAGCGATTTTTTTCAT 
               
               
                   
               
               
                 CCCAGGATATGGCACCTCATGGATCATCATCACATATGTCACAAGGGAC 
               
               
                   
               
               
                 AGATGAAGATCACGAGGAACATGGTCATGATGAAGATGATCCTGAAGAC 
               
               
                   
               
               
                 AGTGATGAAGATGATTCTGATGAGGATAGCGATGAAGATTCAGGGAATGG 
               
               
                   
               
               
                 TCACCAAGTTCATCATACGTCTACCTACATTGACAGGAGAATTCAACCTG 
               
               
                   
               
               
                 TTTTTGATAGCCTCGGCTTGCAAAGATATGTGCTCTTGTGCTCTCAGGT 
               
               
                   
               
               
                 TGCTGATGGTGGATTCAGAGACAAGCTGAGGAAACCCCGTGACTTCTA 
               
               
                   
               
               
                 CCACACATGTTACTGCCTAAGCGGTCTTTCCGTGGCTCAACACGCTTG 
               
               
                   
               
               
                 GTCAAAAGACGAGGACACTCCTCCTTTGACTCGTGACATTTTGGGTGG 
               
               
                   
               
               
                 CTACGCAAACCACCTTGAACCTGTTCACCTCCTCCACAACATTGTCTT 
               
               
                   
               
               
                 GGATCGGTATTATGAAGCTTCTAGATTT 
               
               
                   
               
            
           
         
       
     
     A disclosed FT3 polypeptide (SEQ ID NO: 15) encoded by SEQ ID NO: 13 has 370 amino acid residues and is presented in Table 6B using the one-letter amino acid code. 
     
       
         
           
               
             
               
                 TABLE 6B 
               
               
                   
               
               
                 Encoded FT3 protein sequence (SEQ ID NO: 15). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 WLCYWILHSIALLGESVDDDLENNAIDFLGRCQGSDGGYGGGPGQLPHLA 
               
               
                   
               
               
                 TSYAAVNTLVTLGGEKAFSSINREQMACFLRRMKDTNGGFRMHNMGEIDV 
               
               
                   
               
               
                 RACYTAILIASILNIVDDELTRGLGDYILSCQTYEGGIGGEPGSEAHGGY 
               
               
                   
               
               
                 TYCGLATMILINEVDRLNLDSLMNWVVHRQGVEMGFQGRTNKLVDGCYTF 
               
               
                   
               
               
                 WQAAPCVLLQRFFSSQDMAPHGSSSHMSQGTDEDHEEHGHDEDDPEDSD 
               
               
                   
               
               
                 EDDSDEDSDEDSGNGHQVHHTSTYIDRRIQPVFDSLGLQRYVLLCSQVA 
               
               
                   
               
               
                 DGGFRDKLRKPRDFYHTCYCLSGLSVAQHAWSKDEDTPPLTRDILGGYA 
               
               
                   
               
               
                 NHLEPVHLLHNILVDRYYEASRF 
               
               
                   
               
            
           
         
       
     
     Due to the nature of the cloning strategy the sequence presented is not full length. Compared to the  Arabidopsis thaliana  sequence there are 31 amino acids missing from the amino terminus and 5 amino acids from the carboxy terminus. The percent identity of the  Brassica napus  nucleotide sequence and its encoded amino acid sequence to that of published sequences is shown in  FIG. 18 . 
     Using the sequences disclosed herein as hybridization probes, one is able to screen and isolate full length sequences from cDNA or genomic libraries or use the rapid amplification of cDNA ends (RACE) technology or other such PCR techniques. Sequence comparisons have been performed and percent identities are shown in  FIG. 17  and  FIG. 18 . 
     The present invention also includes a nucleic acid sequence complementary to the  Brassica napsus  farnesyl transferase beta subunit of SEQ ID NO:14. The disclosed complementary sequence is shown as SEQ ID NO:36. 
                    SEQ ID NO: 36                 AAATCTAGAAGCTTCATAATACCGATCCAAGACAATGTTGTGGAGGAGGT               GAACAGGTTCAAGGTGGTTTGCGTAGCCACCCAAAATGTCACGAGTCAAA               GGAGGAGTGTCCTCGTCTTTTGACCAAGCGTGTTGAGCCACGGAAAGACC               GCTTAGGCAGTAACATGTGTGGTAGAAGTCACGGGGTTTCCTCAGCTTGT               CTCTGAATCCACCATCAGCAACCTGAGAGCACAAGAGCACATATCTTTG               CAAGCCGAGGCTATCAAAAACAGGTTGAATTCTCCTGTCAATGTAGGTAG               ACGTATGATGAACTTGGTGACCATTCCCTGAATCTTCATCGCTATCCTCA               TCAGAATCATCTTCATCACTGTCTTCAGGATCATCTTCATCATGACCATG               TTCCTCGTGATCTTCATCTGTCCCTTGTGACATATGTGATGATGATCCAT               GAGGTGCCATATCCTGGGATGAAAAAAATCGCTGTAGTAGAACACAGG               GGGCTGCCTGCCAAAACGTGTAGCAACCGTCGACCAATTTGTTCGTCC               TACCTTGGAATCCCATTTCTACTCCTTGTCGATGTACAACCCAATTCATT               AACGAATCCAAATTCAAGCGGTCGACTTCATTGATTAAAATCATAGTAG               CCAACCCACAGTACGTGTACCCACCATGAGCTTCGGAGCCAGGTTCC               CCTCCAATGCCACCTTCATAAGTTTGGCAACTCAAAATGTAATCTCCTA               AGCCGCGGGTGAGTTCATCATCCACAATGTTCAGGATGCTTGCAATCA               AAATCGCAGTGTAGCACGCTCGCACATCTATTTCTCCCATATTATGCAT               CCTGAAACCTCCATTTGTATCCTTCATTCGTCTTAAGAAACAAGCCATT               TGTTCTCTGTTAATTGAAGAGAAGGCTTTCTCACCTCCTAAAGTAACAA               GTGTATTCACTGCAGCATAACTTGTTGCAAGATGTGGAAGTTGGCCAG               GACCACCACCATATCCACCATCAGAACCCTGGCAACGTCCAAGAAAAT               CGATTGCATTGTTTTCTAAGTCATCATCCACAGACTCCCCAAGCAAAGCA               ATTGAATGAAGAATCCAGTAACAAAGCCA              Glycine max  FTA
 
     A disclosed nucleic acid of 1041 nucleotides (also referred to as FT4) is shown in Table 7A. 
     
       
         
           
               
             
               
                 TABLE 7A 
               
               
                   
               
               
                 FT4 Nucleotide Sequence (SEQ ID NO: 37). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 ATGGAATCTGGGTCTAGCGAAGGAGAAGAGGTGCAGCAACGCGTGCCGTT 
               
               
                   
               
               
                 GAGGGAGAGAGTGGAGTGGTCAGATGTTACTCCGGTTCCTCAAAACGACG 
               
               
                   
               
               
                 GCCCTAACCCTGTCGTTCCGATCCAGTACACTGAAGAGTTTTCCGAAGTT 
               
               
                   
               
               
                 ATGGATTACTTTCGCGCCGTTTACCTCACCGATGAACGCTCCCCTCGCG 
               
               
                   
               
               
                 CCCTCGCTCTCACAGCCGAAGCCGTTCAATTCAACTCCGGCAACTACAC 
               
               
                   
               
               
                 TGTGTGGCATTTCCGACGGTTGTTACTTGAGTCGCTAAAAGTCGACTTG 
               
               
                   
               
               
                 AACGATGAACTGGAGTTTGTGGAGCGTATGGCCGCTGGAAATTCTAAAAA 
               
               
                   
               
               
                 TTATCAGATGTGnATGTTCTGTAGGCATCCTAGACGATGGGTTGCCGAGA 
               
               
                   
               
               
                 AGTTAGGTCCTGAAGCTAGAAACAATGAGCTCGAGTTCACCAAAAAGAT 
               
               
                   
               
               
                 ACTGTCCGTTGATGCCAAACATTATCATGCATGGTCTCATAGACAGTGG 
               
               
                   
               
               
                 GCTCTTCAAACACTAGGAGGATGGGAAGATGAACTTAATTATTGCACAG 
               
               
                   
               
               
                 AACTACTTAAAGAAGACATTTTTAACAATTCTGCTTGGAATCAGAGATAT 
               
               
                   
               
               
                 TTTGTCATAACAAGGTCTCCTTTCTTGGGGGGCCTAAAAGCTATGAGAG 
               
               
                   
               
               
                 AGTCTGAAGTGCTTTACACCATCGAAGCCATTATAGCCTACCCTGAAAA 
               
               
                   
               
               
                 TGAAAGCTCGTGGAGATATCTACGAGGACTTTATAAAGGTGAAACTACTT 
               
               
                   
               
               
                 CATGGGTAAATGATCCTCAAGTTTCTTCAGTATGCTTAAAGATTTTGAGA 
               
               
                   
               
               
                 ACTAAGAGCAACTACGTGTTTGCTCTTAGCACTATTTTAGATCTTATATG 
               
               
                   
               
               
                 CTTTGGTTATCAACCAAATGAAGACATTAGAGATGCCATTGACGCCTTAA 
               
               
                   
               
               
                 AGACCGCAGATATGGATAAACAAGATTTAGATGATGATGAGAAAGGGGAA 
               
               
                   
               
               
                 CAACAAAATTTAAATATAGCACGAAATATTTGTTCTATCCTAAAACAAG 
               
               
                   
               
               
                 TTGATCCAATTAGAACCAACTATTGGATTTGGCGCAAGAGCAGACTTCCT 
               
               
                   
               
            
           
         
       
     
     A disclosed FT4 polypeptide (SEQ ID NO:39) encoded by SEQ ID NO:37 has 347 amino acid residues and is presented in Table 7B using the one-letter amino acid code. 
     
       
         
           
               
             
               
                 TABLE 7B 
               
               
                   
               
               
                 Encoded FT4 protein sequence (SEQ ID NO: 39). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 MESGSSEGEEVQQRVPLRERVEWSDVTPVPQNDGPNPVVPIQYTEEFSEV 
               
               
                   
               
               
                 MDYFRAVYLTDERSPRALALTAEAVQFNSGNYTVWHFRRLLLESLKVDLN 
               
               
                   
               
               
                 DELEFVERMAAGNSKNYQMXMFCRHPRRWVAEKLGPEARNNELEFTKKI 
               
               
                   
               
               
                 LSVDAKHYHAWSHRQWALQTLGGWEDELNYCTELLKEDIFNNSAWNQRYF 
               
               
                   
               
               
                 VITRSPFLGGLKAMRESEVLYTIEAIIAYPENESSWRYLRGLYKGETTSW 
               
               
                   
               
               
                 VNDPQVSSVCLKILRTKSNYVFALSTILDLICFGYQPNEDIRDAIDALKT 
               
               
                   
               
               
                 ADMDKQDLDDDEKGEQQNLNIARNICSILKQVDPIRTNYWIWRKSRLP 
               
               
                   
               
            
           
         
       
     
     Due to the nature of the cloning strategy the sequence presented is not full length. The percent identity of the  Glycine max  nucleotide sequence and its encoded amino acid sequence to that of other sequences is shown in  FIG. 17 . 
     Using the sequences disclosed herein as hybridization probes, one is able to screen and isolate full length sequences from cDNA or genomic libraries or use the rapid amplification of cDNA ends (RACE) technology or other such PCR techniques. 
     The present invention also includes a nucleic acid sequence complementary to the  Glycine max  alpha subunit of SEQ ID NO:37. The disclosed complementary sequence is shown as SEQ ID NO:38. 
                    SEQ ID NO: 38                 AGGAAGTCTGCTCTTGCGCCAAATCCAATAGTTGGTTCTAATTGGATCAA               CTTGTTTTAGGATAGAACAAATATTTCGTGCTATATTTAAATTTTGTTGT               TCCCCTTTCTCATCATCATCTAAATCTTGTTTATCCATATCTGCGGTCTT               TAAGGCGTCAATGGCATCTCTAATGTCTTCATTTGGTTGATAACCAAA               GCATATAAGATCTAAAATAGTGCTAAGAGCAAACACGTAGTTGCTCTT               AGTTCTCAAAATCTTTAAGCATACTGAAGAAACTTGAGGATCATTTACC               CATGAAGTAGTTTCACCTTTATAAAGTCCTCGTAGATATCTCCACGAGC               TTTCATTTTCAGGGTAGGCTATAATGGCTTCGATGGTGTAAAGCACTTCA               GACTCTCTCATAGCTTTTAGGCCCCCCAAGAAAGGAGACCTTGTTATGA               CAAAATATCTCTGATTCCAAGCAGAATTGTTAAAAATGTCTTCTTTAAGT               AGTTCTGTGCAATAATTAAGTTCATCTTCCCATCCTCCTAGTGTTTGAAG               AGCCCACTGTCTATGAGACCATGCATGATAATGTTTGGCATCAACGGAC               AGTATCTTTTTGGTGAACTCGAGCTCATTGTTTCTAGCTTCAGGACCTA               ACTTCTCGGCAACCCATCGTCTAGGATGCCTACAGAACATNCACATC               TGATAATTTTTAGAATTTCCAGCGGCCATACGCTCCACAAACTCCAGT               TCATCGTTCAAGTCGACTTTTAGCGACTCAAGTAACAACCGTCGGAAA               TGCCACACAGTGTAGTTGCCGGAGTTGAATTGAACGGCTTCGGCTGTG               AGAGCGAGGGCGCGAGGGGAGCGTTCATCGGTGAGGTAAACGGCGCGA               AAGTAATCCATAACTTCGGAAAACTCTTCAGTGTACTGGATCGGAACG               ACAGGGTTAGGGCCGTCGTTTTGAGGAACCGGAGTAACATCTGACCAC               TCCACTCTCTCCCTCAACGGCACGCGTTGCTGCACCTCTTCTCCTTCG               CTAGACCCAGATTCCAT              Glycine max  FTB
 
     A disclosed nucleic acid of 1035 nucleotides (also referred to as FT5) is shown in Table 8A. 
     
       
         
           
               
             
               
                 TABLE 8A 
               
               
                   
               
               
                 FT5 Nucleotide Sequence (SEQ ID NO: 40). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 GCCACCATTCCTCGCAACGCCCAAACCCTCATGTTGGAGCTTCAACGCGA 
               
               
                   
               
               
                 TAATCACATGCAGTATGTCTCCAAAGGCCTTCGCCATCTCAGTTCCGCAT 
               
               
                   
               
               
                 TTTCCGTTTTGGACGCTAATCGACCCTGGCTCTGCTACTGGATCTTCCA 
               
               
                   
               
               
                 CTCCATTGCTTTGTTGGGAGAATCCGTCGATGATGAACTCGAAGATAAC 
               
               
                   
               
               
                 GCTATCGATTTTCTTAACCGTTGCCAGGATCCGAATGGTGGATATGCC 
               
               
                   
               
               
                 GGGGGACCAGGCCAGATGCCTCATATTGCCACAACTTATGCTGCTGTT 
               
               
                   
               
               
                 AATTCACTTATTACTTTGGGTGGTGAGAAATCCCTGGCATCAATTAATA 
               
               
                   
               
               
                 GAGATAAACTGTATGGGTTTCTGCGGCGGATGAAGCAACCAAATGGT 
               
               
                   
               
               
                 GGATTCAGGATGCATGATGAAGGTGAAATTGATGTTCGAGCTTGCTAC 
               
               
                   
               
               
                 ACTGCCATTTCTGTTGCAAGTGTTTTGAACATTTTGGATGATGAGCTGA 
               
               
                   
               
               
                 TCCAGAATGTTGGAGACTACATTATAAGCTGTCAAACATATGAGGGTG 
               
               
                   
               
               
                 GCATTGCTGGTGAGCCTGGTTCTGAGGCTCATGGTGGGTACACCTTT 
               
               
                   
               
               
                 TGTGGATTAGCTACAATGATTCTGATTGGTGAGGTTAATCACTTGGAT 
               
               
                   
               
               
                 CTGCCTCGATTAGTTGACTGGGTGGTATTCCGACAAGGTAAGGAATGT 
               
               
                   
               
               
                 GGATTCCAGGGGAGAACAAATAAACTGGTGGATGGATGCTATTCCTTT 
               
               
                   
               
               
                 TGGCAGGGAGGTGCTGTTGCTCTATTGCAAAGATTATCTTCTATTATCA 
               
               
                   
               
               
                 ACAAACAGATGGAAGAGACATCACAGATTTTTGCGGTATCTTATGTAT 
               
               
                   
               
               
                 CTGAAGCAAAAGAAAGTTTGGATGGAACCTCTAGTCATGCAACATGC 
               
               
                   
               
               
                 CGTGGTGAGCATGAAGGCACCAGTGAATCCAGTTCATCTGATTTTAA 
               
               
                   
               
               
                 AAATATTGCCTATAAATTTATTAATGAGTGGAGAGCACAAGAACCAC 
               
               
                   
               
               
                 TTTTTCACAGTATTGCTTTACAGCAATATATTCTCTTATGTGCACAGG 
               
               
                   
               
               
                 AGCAAGAGGGTGGACTGAGAGACAAACCGGGTAAACGTAGAGATC 
               
               
                   
               
               
                 ATTATCACACATGTTACTGTTTAAGTGGACTCTCATTGTGCCAGTATA 
               
               
                   
               
               
                 GTTGGTCAAAGCACCCAGATTCTCCACCAC 
               
               
                   
               
            
           
         
       
     
     A disclosed FT5 polypeptide (SEQ ID NO:42) encoded by SEQ ID NO:40 has 378 amino acid residues and is presented in Table 8B using the one-letter amino acid code. 
     
       
         
           
               
             
               
                 TABLE 8B 
               
               
                   
               
               
                 Encoded FT5 protein sequence (SEQ ID NO: 42). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 ATIPRNAQTLMLELQRDNHMQYVSKGLRHLSSAFSVLDANRPWLCYWIF 
               
               
                   
               
               
                 HSIALLGESVDDELEDNAIDFLNRCQDPNGGYAGGPGQMPHIATTYAAV 
               
               
                   
               
               
                 NSLITLGGEKSLASINRDKLYGFLRRMKQPNGGFRMHDEGEIDVRACYT 
               
               
                   
               
               
                 AISVASVLNILDDELIQNVGDYIISCQTYEGGIAGEPGSEAHGGYTFCGL 
               
               
                   
               
               
                 ATMILIGEVNHLDLPRLVDWVVFRQGKECGFQGRTNKLVDGCYSFWQG 
               
               
                   
               
               
                 GAVALLQRLSSIINKQMEETSQIFAVSYVSEAKESLDGTSSHATCRGEH 
               
               
                   
               
               
                 EGTSESSSSDFKNIAYKFINEWRAQEPLFHSIALQQYILLCAQEQEGGLR 
               
               
                   
               
               
                 DKPGKRRDHYHTCYCLSGLSLCQYSWSKHPDSPP 
               
               
                   
               
            
           
         
       
     
     Due to the nature of the cloning strategy the sequence presented is not full length. The percent identity of the  Glycine max  nucleotide sequence and its encoded amino acid sequence to that of other sequences is shown in  FIG. 17 . 
     Using the sequences disclosed herein as hybridization probes, one is able to screen and isolate full length sequences from cDNA or genomic libraries or use the rapid amplification of cDNA ends (RACE) technology or other such PCR techniques. 
     The present invention also includes a nucleic acid sequence complementary to the  Glycine max  beta subunit of SEQ ID NO:40. The disclosed complementary sequence is shown as SEQ ID NO:41. 
                    SEQ ID NO: 41                 GTGGTGGAGAATCTGGGTGCTTTGACCAACTATACTGGCACAATGAGAG               TCCACTTAAACAGTAACATGTGTGATAATGATCTCTACGTTTACCCGGTT               TGTCTCTCAGTCCACCCTCTTGCTCCTGTGCACATAAGAGAATATATTG               CTGTAAAGCAATACTGTGAAAAAGTGGTTCTTGTGCTCTCCACTCATTA               ATAAATTTATAGGCAATATTTTTAAAATCAGATGAACTGGATTCACTGGT               GCCTTCATGCTCACCACGGCATGTTGCATGACTAGAGGTTCCATCCAA               ACTTTCTTTTGCTTCAGATACATAAGATACCGCAAAAATCTGTGATGTCT               CTTCCATCTGTTTGTTGATAATAGAAGATAATCTTTGCAATAGAGCAAC               AGCACCTCCCTGCCAAAAGGAATAGCATCCATCCACCAGTTTATTTGTT               CTCCCCTGGAATCCACATTCCTTACCTTGTCGGAATACCACCCAGTCA               ACTAATCGAGGCAGATCCAAGTGATTAACCTCACCAATCAGAATCATT               GTAGCTAATCCACAAAAGGTGTACCCACCATGAGCCTCAGAACCAGGCT               CACCAGCAATGCCACCCTCATATGTTTGACAGCTTATAATGTAGTCTCCA               ACATTCTGGATCAGCTCATCATCCAAAATGTTCAAAACACTTGCAACAGA               AATGGCAGTGTAGCAAGCTCGAACATCAATTTCACCTTCATCATGCATCC               TGAATCCACCATTTGGTTGCTTCATCCGCCGCAGAAACCCATACAGTTTA               TCTCTATTAATTGATGCCAGGGATTTCTCACCACCCAAAGTAATAAGTGA               ATTAACAGCAGCATAAGTTGTGGCAATATGAGGCATCTGGCCTGGTCCC               CCGGCATATCCACCATTCGGATCCTGGCAACGGTTAAGAAAATCGATA               GCGTTATCTTCGAGTTCATCATCGACGGATTCTCCCAACAAAGCAATGG               AGTGGAAGATCCAGTAGCAGAGCCAGGGTCGATTAGCGTCCAAAACGGA               AAATGCGGAACTGAGATGGCGAAGGCCTTTGGAGACATACTGCATGTG               ATTATCGCGTTGAAGCTCCAACATGAGGGTTTGGGCGTTGCGAGGAATG               GTGGC              Zea maize  FTB
 
     A disclosed nucleic acid of 1235 nucleotides (also referred to as FT6) is shown in Table 9A. 
     
       
         
           
               
             
               
                 TABLE 9A 
               
               
                   
               
               
                 FT6 Nucleotide Sequence (SEQ ID NO: 43). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 GGCGGATCCCGACCTACCGAGGCTCACGGTGACGCAGGTGGAGCAGAT 
               
               
                   
               
               
                 GAAGGTGGAGGCCAGGGTTGGCGACATCTACCGCTCCCTCTTCGGGGC 
               
               
                   
               
               
                 CGCGCCCAACACGAAATCCATCATGCTAGAGCTGTGGCGTGATCAGCA 
               
               
                   
               
               
                 TATCGAGTATCTGACGCCTGGGCTGAGGCATATGGGACCAGCCTTTCAT 
               
               
                   
               
               
                 GTTCTAGATGCCAATCGCCCTTGGCTATGCTACTGGATGGTTCATCCACT 
               
               
                   
               
               
                 TGCTTTGCTGGATGAAGCACTTGATGATGATCTTGAGAATGATATCATAG 
               
               
                   
               
               
                 ACTTCTTAGCTCGATGTCAGGATAAAGATGGTGGATATAGTGGTGGACC 
               
               
                   
               
               
                 TGGACAGTTGCCTCACCTAGCTACGACTTATGCTGCTGTAAATACACTT 
               
               
                   
               
               
                 GTGACAATAGGGAGCGAAAGAGCATTGTCATCAATCAATAGGGGCAAC 
               
               
                   
               
               
                 CTGTACAATTTTATGCTGCAGATGAAAGATGTATCAGGTGCTTTCAGAAT 
               
               
                   
               
               
                 GCATGATGGTGGCGAAATTGATGTCCGTGCTTCCTACACCGCTATATCG 
               
               
                   
               
               
                 GTTGCCAGCCTTGTGAATATTCTTGATTTTAAACTGGCAAAAGGTGTAGG 
               
               
                   
               
               
                 CGACTACATAGCAAGATGTCAAACTTATGAAGGTGGTATTGCTGGGGAG 
               
               
                   
               
               
                 CCTTATGCTGAAGCACATGGTGGGTATACATTCTGTGGATTGGCTGCTTT 
               
               
                   
               
               
                 GATCCTGCTTAATGAGGCAGAGAAAGTTGACTTGCCTAGTTTGATTGGCT 
               
               
                   
               
               
                 GGGTGGCTTTTCGTCAAGGAGTGGAATGCGGATTTCAAGGACGAACTAA 
               
               
                   
               
               
                 TAAATTGGTTGATGGTTGCTACTCCTTTTGGCAGGGAGCTGCCATTGCTT 
               
               
                   
               
               
                 TCACACAAAAGTTAATTACGATTGTTGATAAGCAATTGAGGTCCTCGTAT 
               
               
                   
               
               
                 TCCTGCAAAAGGCCATCAGGAGAGGATGCCTGCAGCACCAGTTCATAT 
               
               
                   
               
               
                 GGGTGCACCGCGAATAAGTCTTCCTCTGCTGTGGACTATGCGAAGTTTG 
               
               
                   
               
               
                 GATTTGATTTTATACAACAGAGCAACCAAATTGGCCCACTCTTCCATAAC 
               
               
                   
               
               
                 ATTGCCCTGCAACAATACATCCTACTTTGTTCTCAGGTACTAGAGGGAG 
               
               
                   
               
               
                 GCTTGAGGGATAAGCCTGGAAAGAACAGAGATCACTATCATTCATGCTA 
               
               
                   
               
               
                 CTGCCTCAGTGGCCTCGCAGTTAGCCAGTACAGTGCCATGACTGATACT 
               
               
                   
               
               
                 GGTTCGTGCCCATTACCTCAGCATGTGCTTGGACCGTACTCTAATTTGCT 
               
               
                   
               
               
                 GGAGCCAATCCATCC 
               
               
                   
               
            
           
         
       
     
     A disclosed FT6 polypeptide (SEQ ID NO:45) encoded by SEQ ID NO:43 has 414 amino acid residues and is presented in Table 9B using the one-letter amino acid code. 
     
       
         
           
               
             
               
                 TABLE 9B 
               
               
                   
               
               
                 Encoded FT6 protein sequence (SEQ ID NO: 45). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 ADPDLPRLTVTQVEQMKVEARVGDIYRSLFGAAPNTKSIMLELWRDQHIE 
               
               
                   
               
               
                 YLTPGLRHMGPAFHVLDANRPWLCYWMVHPLALLDEALDDDLENDIID 
               
               
                   
               
               
                 FLARCQDKDGGYSGGPGQLPHLATTYAAVNTLVTIGSERALSSINRGNL 
               
               
                   
               
               
                 YNFMLQMKDVSGAFRMHDGGEIDVRASYTAISVASLVNILDFKLAKGVG 
               
               
                   
               
               
                 DYIARCQTYEGGIAGEPYAEAHGGYTFCGLAALILLNEAEKVDLPSLIGW 
               
               
                   
               
               
                 VAFRQGVECGFQGRTNKLVDGCYSFWQGAAIAFTQKLITIVDKQLRSSY 
               
               
                   
               
               
                 SCKRPSGEDACSTSSYGCTANKSSSAVDYAKFGFDFIQQSNQIGPLFH 
               
               
                   
               
               
                 NIALQQYILLCSQVLEGGLRDKPGKNRDHYHSCYCLSGLAVSQYSAMTD 
               
               
                   
               
               
                 TGSCPLPQHVLGPYSNLLEPIH 
               
               
                   
               
            
           
         
       
     
     Due to the nature of the cloning strategy the sequence presented is not full length. The percent identity of the  Glycine max  nucleotide sequence and its encoded amino acid sequence to that of other sequences is shown in  FIG. 17 . 
     Using the sequences disclosed herein as hybridization probes, one is able to screen and isolate full length sequences from cDNA or genomic libraries or use the rapid amplification of cDNA ends (RACE) technology or other such PCR techniques. 
     The present invention also includes a nucleic acid sequence complementary to the  Zea maize  beta subunit of SEQ ID NO:43. The disclosed complementary sequence is shown as SEQ ID NO:44. 
     
       
         
           
               
            
               
                 SEQ ID NO: 44 
               
            
           
           
               
            
               
                 GGATGGATTGGCTCCAGCAAATTAGAGTACGGTCCAAGCACATGCTGAG 
               
               
                   
               
               
                 GTAATGGGCACGAACCAGTATCAGTCATGGCACTGTACTGGCTAACTGC 
               
               
                   
               
               
                 GAGGCCACTGAGGCAGTAGCATGAATGATAGTGATCTCTGTTCTTTCCAG 
               
               
                   
               
               
                 GCTTATCCCTCAAGCCTCCCTCTAGTACCTGAGAACAAAGTAGGATGTAT 
               
               
                   
               
               
                 TGTTGCAGGGCAATGTTATGGAAGAGTGGGCCAATTTGGTTGCTCTGTTG 
               
               
                   
               
               
                 TATAAAATCAAATCCAAACTTCGCATAGTCCACAGCAGAGGAAGACTTA 
               
               
                   
               
               
                 TTCGCGGTGCACCCATATGAACTGGTGCTGCAGGCATCCTCTCCTGATG 
               
               
                   
               
               
                 GCCTTTTGCAGGAATACGAGGACCTCAATTGCTTATCAACAATCGTAATT 
               
               
                   
               
               
                 AACTTTTGTGTGAAAGCAATGGCAGCTCCCTGCCAAAAGGAGTAGCAAC 
               
               
                   
               
               
                 CATCAACCAATTTATTAGTTCGTCCTTGAAATCCGCATTCCACTCCTTGA 
               
               
                   
               
               
                 CGAAAAGCCACCCAGCCAATCAAACTAGGCAAGTCAACTTTCTCTGCC 
               
               
                   
               
               
                 TCATTAAGCAGGATCAAAGCAGCCAATCCACAGAATGTATACCCACCA 
               
               
                   
               
               
                 TGTGCTTCAGCATAAGGCTCCCCAGCAATACCACCTTCATAAGTTTGAC 
               
               
                   
               
               
                 ATCTTGCTATGTAGTCGCCTACACCTTTTGCCAGTTTAAAATCAAGAATA 
               
               
                   
               
               
                 TTCACAAGGCTGGCAACCGATATAGCGGTGTAGGAAGCACGGACATCA 
               
               
                   
               
               
                 ATTTCGCCACCATCATGCATTCTGAAAGCACCTGATACATCTTTCATCTG 
               
               
                   
               
               
                 CAGCATAAAATTGTACAGGTTGCCCCTATTGATTGATGACAATGCTCTTT 
               
               
                   
               
               
                 CGCTCCCTATTGTCACAAGTGTATTTACAGCAGCATAAGTCGTAGCTAG 
               
               
                   
               
               
                 GTGAGGCAACTGTCCAGGTCCACCACTATATCCACCATCTTTATCCTGA 
               
               
                   
               
               
                 CATCGAGCTAAGAAGTCTATGATATCATTCTCAAGATCATCATCAAGTGC 
               
               
                   
               
               
                 TTCATCCAGCAAAGCAAGTGGATGAACCATCCAGTAGCATAGCCAAGG 
               
               
                   
               
               
                 GCGATTGGCATCTAGAACATGAAAGGCTGGTCCCATATGCCTCAGCCC 
               
               
                   
               
               
                 AGGCGTCAGATACTCGATATGCTGATCACGCCACAGCTCTAGCATGAT 
               
               
                   
               
               
                 GGATTTCGTGTTGGGCGCGGCCCCGAAGAGGGAGCGGTAGATGTCGC 
               
               
                   
               
               
                 CAACCCTGGCCTCCACCTTCATCTGCTCCACCTGCGTCACCGTGAGC 
               
               
                   
               
               
                 CTCGGTAGGTCGGGATCCGCC 
               
            
           
         
       
     
     The FTA and FTB nucleic acids and amino acids disclosed above have homology to other members of the FT protein family (GenBank ID NOs: U63298, U83707, and U73203; WO 00/14207; Cutler et al., Science 273(5279):1239-41, 1996; Ziegelhoffer et al., Proc Natl Acad Sci USA. 97(13):7633-8, 2000). The homology between these and other sequences is shown graphically in the ClustalW analysis shown in Tables 10A-10D. In the ClustalW alignment, the black outlined amino acid residues indicate regions of conserved sequence (i.e., regions that may be required to preserve structural or functional properties), whereas non-highlighted amino acid residues are less conserved and can potentially be altered to a much broader extent without altering protein structure or function. 
     
       
         
           
               
             
               
                 TABLE 10A 
               
               
                   
               
               
                 ClustalW Nucleic Acid Analysis of FT Alpha Subunits 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) BNA-12; FT2 (SEQ ID NO: 12) 
               
               
                 2) At-FT-A; FT1 (SEQ ID NO: 7) 
               
               
                 3) PPI-Soy-FTA; FT4 (SEQ ID NO: 37) 
               
               
                 4) Pea-FT-A (SEQ ID NO: 65) 
               
               
                 5) Tomato-FTA (SEQ ID NO: 66) 
               
               
                 6) Rice-FT-A (SEQ ID NO: 67) 
               
               
                 7) Zea mays-FT-A (SEQ ID NO: 68) 
               
               
                 8) Soy1-FT-A (SEQ ID NO: 69) 
               
               
                 9) Soy2-FT-A (SEQ ID NO: 70) 
               
               
                 10) Triticum-FT-A (SEQ ID NO: 71) 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10B 
               
               
                   
               
               
                 ClustalW Amino Acid Analysis of FT Alpha Subunits 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) BNA-12; FT2 (SEQ ID NO: 13) 
               
               
                 2) At-FT-A; FT1 (SEQ ID NO: 11) 
               
               
                 3) PPI-Soy-FTA; FT4 (SEQ ID NO: 39) 
               
               
                 4) Pea-FT-A (SEQ ID NO: 72) 
               
               
                 5) Tomato-FTA (SEQ ID NO: 73) 
               
               
                 6) Rice-FT-A (SEQ ID NO: 74) 
               
               
                 7) Zea mays-FT-A (SEQ ID NO: 75) 
               
               
                 8) Soy1-FT-A (SEQ ID NO: 76) 
               
               
                 9) Soy2-FT-A (SEQ ID NO: 77) 
               
               
                 10) Triticum-FT-A (SEQ ID NO: 78) 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10C 
               
               
                   
               
               
                 ClustalW Nucleic Acid Analysis of FT Beta Subunits 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) PPI-BnFTb; FT3 (SEQ ID NO: 14) 
               
               
                 2) eral (SEQ ID NO: 1) 
               
               
                 3) Wiggum (SEQ ID NO: 80) 
               
               
                 4) PPI-Soy-FTB; FT5 (SEQ ID NO: 40) 
               
               
                 5) DuP-Soy-FTB (SEQ ID NO: 81) 
               
               
                 6) PPI-Corn-FTB; FT6 (SEQ ID NO: 43) 
               
               
                 7) DuP-Corn-FTB (SEQ ID NO: 82) 
               
               
                 8) Pea-FT-B (SEQ ID NO: 83) 
               
               
                 9) Tomato (SEQ ID NO: 84) 
               
               
                 10) Tobacco (SEQ ID NO: 85) 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10D 
               
               
                   
               
               
                 ClustalW Amino Acid Analysis of FT Beta Subunits 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                  1) PPI-BnFTB; FT3 (SEQ ID NO: 15) 
               
               
                  2) eral (SEQ ID NO: 2) 
               
               
                  3) Wiggum (SEQ ID NO: 87) 
               
               
                  4) PPI-Soy-FTB; FT5 (SEQ ID NO: 42) 
               
               
                  5) DuP-Soy-FTB (SEQ ID NO: 88) 
               
               
                  6) PPI-Corn-FTB; FT6 (SEQ ID NO: 45) 
               
               
                  7) DuP-Com-FTB (SEQ ID NO: 89) 
               
               
                  8) Pea-FT-B (SEQ ID NO: 90) 
               
               
                  9) Tomato (SEQ ID NO: 91) 
               
               
                 10) Tobacco (SEQ ID NO: 92) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Also included in the invention is the farnesyl transferase alpha consensus sequence of SEQ ID NO:93 and the farnesyl transferase beta consensus sequence of SEQ ID NO:94 To generate the consensus sequence, the farnesyl transferase alpha and farnesyl transferase beta sequences of the invention were aligned using the program BioEdit. The homology between the farnesyl transferase alpha (FTA) polypeptide sequences of the invention is shown graphically in the ClustalW analysis shown in Table 10E. The homology between the farnesyl transferase beta (FTB) polypeptide sequences of the invention is shown graphically in the ClustalW analysis shown in Table 10F. 
     
       
         
           
               
             
               
                 TABLE 10E 
               
               
                   
               
               
                 ClustalW Amino Acid Analysis of FT Alpha 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10F 
               
               
                   
               
               
                 ClustalW Amino Acid Analysis of FT Beta 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Also included in the invention is the farnesyl transferase alpha consensus sequence of SEQ ID NO:95 and the farnesyl transferase beta consensus sequence of SEQ ID NO:96. To generate the consensus sequence, the farnesyl transferase alpha and farnesyl transferase beta sequences of the invention were aligned using the program BioEdit. The homology between the farnesyl transferase alpha (FTA) nucleic acid sequences of the invention is shown graphically in the ClustalW analysis shown in Table 10G. The homology between the farnesyl transferase beta (FTB) nucleic acid sequences of the invention is shown graphically in the ClustalW analysis shown in Table 10H. 
     
       
         
           
               
             
               
                 TABLE 10G 
               
               
                   
               
               
                 ClustalW Nucleic Acid Analysis of FT Alpha 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10H 
               
               
                   
               
               
                 ClustalW Nucleic Acid Analysis of FT Beta 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 13 
     Vector constructs for Transformation 
     The FTA or FTB sequences have be used to produce constructs suitable for transformation into plants and under the control of appropriate regulatory sequences. The gene sequences were in either the sense orientation for over-expression or the antisense orientation for down-regulation. Portions of these sequences have been used to construct a double-stranded-RNA-inhibition (dsRNAi) construct. A sequence of preferably not less than 21 nt was cloned as an inverse repeat separated by a linker that when expressed results in down-regulation of the target gene. Double antisense (DA) vectors have been created in which a direct repeat of an antisense sequence is separated by a spacer sequence such as GUS. Promoters have been used for constitutive expression such as the 35S CaMV promoter, the MuA  Zea maize  promoter or inducible by specific environmental or cellular cues such as the ABA levels or drought conditions which induce expression of the RD29A promoter. Alternatively, tissue or organelle specific promoters such as the HIC or CUT1 promoter can be used. Such constructs have been transformed into  Arabidopsis thaliana, Brassica, Zea maize, Glycine max . Other species can be transformed as desired. Each species to be transformed may make use of specific regulatory sequences as appropriate for those particular species. Transformed plants have be selected and their phenotypic properties analyzed. The transgenic plants were assessed for characteristics such as increased tolerance to drought, altered biomass accumulation, yield, nutritional requirements such as minerals or micro-nutrients, biotic stress such as fungal, bacterial, or other such pathogen infection or attack or any other such physical or biochemical characteristic. 
     Example 14 
     Plant Transformation 
       Arabidopsis thaliana  transgenic plants were made by flower dipping method into an  Agrobacterium  culture. Wild type plants were grown under standard conditions until they began flowering. The plant was inverted for 2 min into a solution of  Agrobacterium  culture. Plants were then bagged for two days to maintain humidity and then uncovered to continue growth and seed development. Mature seed was bulk harvested. 
     Transformed T1 plants were selected by germination and growth on MS plates containing 50 μg/ml kanamycin. Green, kanamycin resistant seedlings were identified after 2 weeks growth and transplanted to soil. Plants were bagged to ensure self fertilization and the T2 seed of each plant harvested separately. During growth of T1 plants leaf samples were harvested, DNA extracted and Southern analysis performed. 
     T2 seeds were analyzed for Kan R  segregation. From those lines that showed a 3:1 resistant phenotype surviving T2 plants were grown, bagged during seed set, and T3 seed harvested from each line. T3 seed was again used for Kan R  segregation analysis and those lines showing 100% Kan R  phenotype were selected as homozygous lines. Further analysis was done using T3 seed. 
     Transgenic  Brassica napus  plants were produced using  Agrobacterium  mediated transformation of cotyledon petiole tissue. Seeds were sterilized as follows. Seeds were wetted with 95% ethanol for a short period of time such as 15 seconds. Approximately 30 ml of sterilizing solution I was added (70% Javex, 100 μl Tween20) and left for approximately 15 minutes. Solution I was removed and replaced with 30 ml of solution II (0.25% mecuric chloride, 100 μl Tween20) and incubated for about 10 minutes. Seeds were rinsed with at least 500 ml double distilled sterile water and stored in a sterile dish. Seeds were germinated on plates of ½ MS medium, pH 5.8, supplemented with 1% sucrose and 0.7% agar. Fully expanded cotyledons were harvested and placed on Medium I (Murashige minimal organics (MMO), 3% sucrose, 4.5 mg/L benzyl adenine (BA), 0.7% phytoagar, pH5.8). An  Agrobacterium  culture containing the nucleic acid construct of interest was grown for 2 days in AB Minimal media. The cotyledon explants were dipped such that only the cut portion of the petiole is contacted by the  Agrobacterium  solution. The explants were then embedded in Medium I and maintained for 5 days at 24° C., with 16.8 hr light dark cycles. Explants were transferred to Medium II (Medium I, 300 mg/L timentin) for a further 7 days and then to Medium III (Medium II, 20 mg/L kanamycin). Any root or shoot tissue which had developed at this time was dissected away. Transfer explants to fresh plates of Medium III after 14-21 days. When regenerated shoot tissue developed the regenerated tissue was transferred to Medium IV (MMO, 3% sucrose, 1.0% phytoagar, 300 mg/L timentin, 20 mg/L 20 mg/L kanamycin). Once healthy shoot tissue developed shoot tissue dissected from any callus tissue was dipped in 10×IBA and transferred to Medium V (Murashige and Skooge (MS), 3% sucrose, 0.2 mg/L indole butyric acid (IBA), 0.7% agar, 300 mg/L timentin, 20 mg/L 20 mg/L kanamycin) for rooting. Healthy plantlets were transferred to soil. 
     Transgenic  Glycine max, Zea maize  and cotton can be produced using  Agrobacterium -based methods which are known to one of skill in the art. Alternatively one can use a particle or non-particle biolistic bombardment transformation method. An example of non-particle biolistic transformation is given in U.S. Patent Application 20010026941. Viable plants are propogated and homozygous lines are generated. Plants are tested for the presence of drought tolerance, physiological and biochemical phenotypes as described elsewhere. 
     The following table identifies the constructs and the species which they have been transformed. 
     
       
         
           
               
               
               
             
               
                 TABLE 11 
               
               
                   
               
               
                 SEQ ID 
                   
                   
               
               
                 NO: 
                 SEQ 
                 Species Transformed 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 SEQ ID 
                 pBI121-35S-anti-AtFTA 
                 
                   Arabidopsis 
                 
                   
               
               
                 NO: 10 
                   
                 
                   thaliana 
                 
                   
               
               
                 SEQ ID 
                 pBI121-35S-AtFTA 
                 
                   Arabidopsis 
                 
                 
                   Brassica 
                 
               
               
                 NO: 46 
                   
                 
                   thaliana 
                 
                 
                   napus 
                 
               
               
                 SEQ ID 
                 pBI121-rd29A-anti-AtFTA 
                 
                   Arabidopsis 
                 
                 
                   Brassica 
                 
               
               
                 NO: 47 
                   
                 
                   thaliana 
                 
                 
                   napus 
                 
               
               
                 SEQ ID 
                 pBI121-35S-DA-AtFTA 
                 
                   Arabidopsis 
                 
                 
                   Brassica 
                 
               
               
                 NO: 48 
                   
                 
                   thaliana 
                 
                 
                   napus 
                 
               
               
                 SEQ ID 
                 pBI121-RD29A-DA-AtFTA 
                 
                   Arabidopsis 
                 
                 
                   Brassica 
                 
               
               
                 NO: 49 
                   
                 
                   thaliana 
                 
                 
                   napus 
                 
               
               
                 SEQ ID 
                 MuA-anti-GmFTA 
                 
                   Glycine 
                 
                   
               
               
                 NO: 50 
                   
                 
                   max 
                 
                   
               
               
                 SEQ ID 
                 RD29A-anti-GmFTA 
                 
                   Glycine 
                 
                   
               
               
                 NO: 51 
                   
                 
                   max 
                 
                   
               
               
                 SEQ ID 
                 MuA-HP-GmFTA-Nos-Term 
                 
                   Glycine 
                 
                   
               
               
                 NO: 52 
                   
                 
                   max 
                 
                   
               
               
                 SEQ ID 
                 RD29AP-HP-GmFTA-Nos- 
                 
                   Glycine 
                 
                   
               
               
                 NO: 53 
                 Term 
                 
                   max 
                 
                   
               
               
                 SEQ ID 
                 pBI121-35S-Anti-AtFTB 
                 
                   Arabidopsis 
                 
                 
                   Brassica 
                 
               
               
                 NO: 54 
                   
                 
                   thaliana 
                 
                 
                   napus 
                 
               
               
                 SEQ ID 
                 pBI121-RD29AP-Anti-AtFTB 
                 
                   Arabidopsis 
                 
                 
                   Brassica 
                 
               
               
                 NO: 55 
                   
                 
                   thaliana 
                 
                 
                   napus 
                 
               
               
                 SEQ ID 
                 pBI121-35S-HP-AtFTB 
                 
                   Arabidopsis 
                 
                 
                   Brassica 
                 
               
               
                 NO: 56 
                   
                 
                   thaliana 
                 
                 
                   napus 
                 
               
               
                 SEQ ID 
                 pBI121-RD29AP-HP-AtFTB 
                 
                   Arabidopsis 
                 
                 
                   Brassica 
                 
               
               
                 NO: 57 
                   
                 
                   thaliana 
                 
                 
                   napus 
                 
               
               
                 SEQ ID 
                 pBI121-35S-AtFTB 
                 
                   Arabidopsis 
                 
                   
               
               
                 NO: 58 
                   
                 
                   thaliana 
                 
                   
               
               
                 SEQ ID 
                 MuA-anti-GmFTB-Nos-Term 
                 
                   Glycine 
                 
                   
               
               
                 NO: 59 
                   
                 
                   max 
                 
                   
               
               
                 SEQ ID 
                 RD29AP-anti-GmFTB-Nos- 
                 
                   Glycine 
                 
                   
               
               
                 NO: 60 
                 Term 
                 
                   max 
                 
                   
               
               
                 SEQ ID 
                 MuA-HP-GmFTB-Nos-Term 
                 
                   Glycine 
                 
                   
               
               
                 NO: 61 
                   
                 
                   max 
                 
                   
               
               
                 SEQ ID 
                 RD29AP-HP-GmFTB-Nos- 
                 
                   Glycine 
                 
                   
               
               
                 NO: 62 
                 Term 
                 
                   max 
                 
                   
               
               
                 SEQ ID 
                 MuA-anti-Zea maizeFTB-Nos- 
                 
                   Zea 
                 
                   
               
               
                 NO: 63 
                 Term 
                 
                   maize 
                 
                   
               
               
                 SEQ ID 
                 MuA-HP-Zea maizeFTB-Nos- 
                 
                   Zea 
                 
                   
               
               
                 NO: 64 
                 Term 
                 
                   maize 
                 
               
               
                   
               
            
           
         
       
     
     Non-limiting examples of vector constructs suitable for plant transformation are given in SEQ ID NO: 10, 46-64. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 10 
                   
               
            
           
           
               
               
            
               
                 
                   
                   aaacactgatagtttaaactgaaggcgggaaacgacaatctgatcatgagcgg 
                 
                   
               
               
                   
               
               
                 
                   agaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttggaactgacagaaccg 
                 
               
               
                   
               
               
                 
                   caacgttgaaggagccactcagccgcgggtttctggagtttaatgagctaagcacatacgtcagaaaccattattgc 
                 
               
               
                   
               
               
                 
                   gcgttcaaaagtcgcctaaggtcactatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaa 
                 
               
               
                   
               
               
                 
                   ttcccctcggtatccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgca 
                 
               
               
                   
               
               
                 
                   tgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaa 
                 
               
               
                   
               
               
                 
                   cagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccga 
                 
               
               
                   
               
               
                 
                   cctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcg 
                 
               
               
                   
               
               
                 
                   cagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctg 
                 
               
               
                   
               
               
                 
                   tcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggc 
                 
               
               
                   
               
               
                 
                   tacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatc 
                 
               
               
                   
               
               
                 
                   aggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgac 
                 
               
               
                   
               
               
                 
                   ggcgatgatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggatt 
                 
               
               
                   
               
               
                 
                   catcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagc 
                 
               
               
                   
               
               
                 
                   ttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctat 
                 
               
               
                   
               
               
                 
                   cgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgacgcccaacctgccatca 
                 
               
               
                   
               
               
                 
                   cgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgat 
                 
               
               
                   
               
               
                 
                   cctccagcgcggggatctcatgctggagttcttcgcccacgggatctctgcggaacaggcggtcgaaggtgccgata 
                 
               
               
                   
               
               
                 
                   tcattacgacagcaacggccgacaagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatc 
                 
               
               
                   
               
               
                 
                   aacggcgtcggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcgtgga 
                 
               
               
                   
               
               
                 
                   gttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttg 
                 
               
               
                   
               
               
                 
                   ccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacg 
                 
               
               
                   
               
               
                 
                   ttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcg 
                 
               
               
                   
               
               
                 
                   cgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggc 
                 
               
               
                   
               
               
                 
                   tctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctctgaggg 
                 
               
               
                   
               
               
                 
                   aggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatga 
                 
               
               
                   
               
               
                 
                   ccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgct 
                 
               
               
                   
               
               
                 
                   gctatcgatggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaa 
                 
               
               
                   
               
               
                 
                   ttcccaaatggctcaagtcggtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctcc 
                 
               
               
                   
               
               
                 
                   ctcaatcggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccgattcat 
                 
               
               
                   
               
               
                 
                   taatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcac 
                 
               
               
                   
               
               
                 
                   tcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc 
                 
               
               
                   
               
               
                 
                   acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag 
                   
                     cccacagatggttagagaggcttacgc 
                   
                 
               
               
                   
               
               
                 
                   
                     agcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatg 
                   
                 
               
               
                   
               
               
                 
                   
                     cagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattcca 
                   
                 
               
               
                   
               
               
                 
                   
                     gtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccac 
                   
                 
               
               
                   
               
               
                 
                   
                     tgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttca 
                   
                 
               
               
                   
               
               
                 
                   
                     tacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactcc 
                   
                 
               
               
                   
               
               
                 
                   
                     aaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacct 
                   
                 
               
               
                   
               
               
                 
                   
                     cctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgcc 
                   
                 
               
               
                   
               
               
                 
                   
                     atcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacg 
                   
                 
               
               
                   
               
               
                 
                   
                     aggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgt 
                   
                 
               
               
                   
               
               
                 
                   
                     aagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaa 
                   
                 
               
               
                   
               
               
                     cacgggg     gactctagaggatcc tcaaattgctgccactgtaatcttgctcttcctccatgcccaatagttagctctt 
               
               
                   
               
               
                 ataggatctacacgaccaagaatagtacacaccaaattggccaagttagtctctggttcttcattagctagagctct 
               
               
                   
               
               
                 cactgagtctttatgctcgttggttggtctcagtccatcacatagaagatccaaaagggtgctcagagcgaatccat 
               
               
                   
               
               
                 ggaagcaatctgtgcgggatagaacattcaaacagactgaggaaacacttggatcactaatccaggattctttgtcg 
               
               
                   
               
               
                 tctttgtaaagcgcttttaggtatcgccatgagctctcgtttgcaggattggttaaaatggctttgattgtgtagct 
               
               
                   
               
               
                 tacttcagattctctcatggcttctaggcctcccaacaaaggagattgggtgatgacataatacctctgattccagg 
               
               
                   
               
               
                 cggaattgttaaagacgtcagcttcaaggagctcgtgacagtaatcgagctcatcttcccatcctcctaatgcccgt 
               
               
                   
               
               
                 agtgtccactgcctatgtgaccaagcatgataatgtttggcatcaagtgaaagtactctacgggtaaattcaagttc 
               
               
                   
               
               
                 tctccctgcaacatcaggacccagtttctctgcaacccatcgccgatgatgccacagttggtagttcttagagttat 
               
               
                   
               
               
                 cctcagcaatgcgttcgatgaactcgagttcttcaaacaagtcgtgattaagggcctcgagtactaggcgcctgaaa 
               
               
                   
               
               
                 tgccacactgtgtagttgccggagtttaagaggagggtttcttccgtgagtcgtagtgcgcgaggagatcgctcgtc 
               
               
                   
               
               
                 ggaaaagtaaatcgcacggaagtaatccatagtctcgcggaactcttccttgtaggcaattggcaccactggattcg 
               
               
                   
               
               
                 gaccatcgtcctgagtcaatgggaccacgtctgaccactccaatcgttggctcagtggcacggtctcgtcgaaattc 
               
               
                   
               
               
                 atccc ctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgc   
               
               
                   
               
               
                 
                   gatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatga 
                 
               
               
                   
               
               
                 
                   gatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactag 
                 
               
               
                   
               
               
                 
                   gataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcactggccgtcgttttacaacgtcgtgact 
                 
               
               
                   
               
               
                 
                   gggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagag 
                 
               
               
                   
               
               
                 
                   gcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttc 
                 
               
               
                   
               
               
                 
                   tcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacgg 
                 
               
               
                   
               
               
                 
                   cacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccc 
                 
               
               
                   
               
               
                 
                   tttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggct 
                 
               
               
                   
               
               
                 
                   attcttttgatttataagggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaacca 
                 
               
               
                   
               
               
                 
                   gcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaa 
                 
               
               
                   
               
               
                 
                   agaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt 
                   
                 
               
               
                   
               
               
                 
                   
                 
               
            
           
         
       
     
     SEQ ID NO: 10 is the nucleic acid sequence of pBI121-antisense-FTA vector construct used to transform  Arabidopsis  plants. Italicized sequences are the right and left border repeats (1-24, 5226-5230). Underlined sequence is the 35S promoter (2515-3318). Bold sequence is the anti-sense Farnesyl transferase alpha sequence (3334-4317). 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 46 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatcca tgaatttcgacgagaccgtgccactgagccaacgattggagtggtcagacgtggtccc   
               
               
                   
               
               
                 
                   attgactcaggacgatggtccgaatccagtggtgccaattgcctacaaggaagagttccgcgag 
                 
               
               
                   
               
               
                 
                   actatggattacttccgtgcgatttacttttccgacgagcgatctcctcgcgcactacgactca 
                 
               
               
                   
               
               
                 
                   cggaagaaaccctcctcttaaactccggcaactacacagtgtggcatttcaggcgcctagtact 
                 
               
               
                   
               
               
                 
                   cgaggcccttaatcacgacttgtttgaagaactcgagttcatcgaacgcattgctgaggataac 
                 
               
               
                   
               
               
                 
                   tctaagaactaccaactgtggcatcatcggcgatgggttgcagagaaactgggtcctgatgttg 
                 
               
               
                   
               
               
                 
                   cagggagagaacttgaatttacccgtagagtactttcacttgatgccaaacattatcatgcttg 
                 
               
               
                   
               
               
                 
                   gtcacataggcagtggacactacgggcattaggaggatgggaagatgagctcgattactgtcac 
                 
               
               
                   
               
               
                 
                   gagctccttgaagctgacgtctttaacaattccgcctggaatcagaggtattatgtcatcaccc 
                 
               
               
                   
               
               
                 
                   aatctcctttgttgggaggcctagaagccatgagagaatctgaagtaagctacacaatcaaagc 
                 
               
               
                   
               
               
                 
                   cattttaaccaatcctgcaaacgagagctcatggcgatacctaaaagctctttacaaagacgac 
                 
               
               
                   
               
               
                 
                   aaagaatcctggattagtgatccaagtgtttcctcagtctgtttgaatgttctatcccgcacag 
                 
               
               
                   
               
               
                 
                   attgcttccatggattcgctctgagcacccttttggatcttctatgtgatggactgagaccaac 
                 
               
               
                   
               
               
                 
                   caacgagcataaagactcagtgagagctctagctaatgaagaaccagagactaacttggccaat 
                 
               
               
                   
               
               
                 
                   ttggtgtgtactattcttggtcgtgtagatcctgtaagagctaactattgggcatggaggaaga 
                 
               
               
                   
               
               
                   gcaagattacagtggcagcaatttga ctcgaatttccccgatcgttcaaacatttggcaataaa 
               
               
                   
               
               
                 gtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaatta 
               
               
                   
               
               
                 cgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgatt 
               
               
                   
               
               
                 agagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggata 
               
               
                   
               
               
                 aattatcgcgcgcggtgtcatctatgttactagatcgggaattcactggccgtcgttttacaac 
               
               
                   
               
               
                 gtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgc 
               
               
                   
               
               
                 cagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaat 
               
               
                   
               
               
                 ggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaa 
               
               
                   
               
               
                 gctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaa 
               
               
                   
               
               
                 aacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgcccttt 
               
               
                   
               
               
                 gacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccct 
               
               
                   
               
               
                 atctcgggctattcttttgatttataagggattttgccgatttcggaaccaccatcaaacagga 
               
               
                   
               
               
                 ttttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtg 
               
               
                   
               
               
                 aagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccccagtacattaaaaac 
               
               
                   
               
               
                 gtccgcaatgtgttattaagttgtctaagcgtcaattt gtttacaccacaatatatcctgcca   
               
               
                   
               
               
                 (Underlined Seq: 35S promoter; Bold: AtFTA) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 47 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaagggtttgattacttctattggaaag actctagaggatcc 
               
               
                   
               
               
                 
                   tcaaattgctgccactgtaatcttgctcttcctccatgcccaatagttagctcttataggatct 
                 
               
               
                   
               
               
                 
                   acacgaccaagaatagtacacaccaaattggccaagttagtctctggttcttcattagctagag 
                 
               
               
                   
               
               
                 
                   ctctcactgagtctttatgctcgttggttggtctcagtccatcacatagaagatccaaaagggt 
                 
               
               
                   
               
               
                 
                   gctcagagcgaatccatggaagcaatctgtgcgggatagaacattcaaacagactgaggaaaca 
                 
               
               
                   
               
               
                 
                   cttggatcactaatccaggattctttgtcgtctttgtaaagcgcttttaggtatcgccatgagc 
                 
               
               
                   
               
               
                 
                   tctcgtttgcaggattggttaaaatggctttgattgtgtagcttacttcagattctctcatggc 
                 
               
               
                   
               
               
                 
                   ttctaggcctcccaacaaaggagattgggtgatgacataatacctctgattccaggcggaattg 
                 
               
               
                   
               
               
                 
                   ttaaagacgtcagcttcaaggagctcgtgacagtaatcgagctcatcttcccatcctcctaatg 
                 
               
               
                   
               
               
                 
                   cccgtagtgtccactgcctatgtgaccaagcatgataatgtttggcatcaagtgaaagtactct 
                 
               
               
                   
               
               
                 
                   acgggtaaattcaagttctctccctgcaacatcaggacccagtttctctgcaacccatcgccga 
                 
               
               
                   
               
               
                 
                   tgatgccacagttggtagttcttagagttatcctcagcaatgcgttcgatgaactcgagttctt 
                 
               
               
                   
               
               
                 
                   caaacaagtcgtgattaagggcctcgagtactaggcgcctgaaatgccacactgtgtagttgcc 
                 
               
               
                   
               
               
                 
                   ggagtttaagaggagggtttcttccgtgagtcgtagtgcgcgaggagatcgctcgtcggaaaag 
                 
               
               
                   
               
               
                 
                   taaatcgcacggaagtaatccatagtctcgcggaactcttccttgtaggcaattggcaccactg 
                 
               
               
                   
               
               
                 
                   gattcggaccatcgtcctgagtcaatgggaccacgtctgaccactccaatcgttggctcagtgg 
                 
               
               
                   
               
               
                   cacggtctcgtcgaaattcatccc ctcgaatttccccgatcgttcaaacatttggcaataaagt 
               
               
                   
               
               
                 ttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacg 
               
               
                   
               
               
                 ttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattag 
               
               
                   
               
               
                 agtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaa 
               
               
                   
               
               
                 ttatcgcgcgcggtgtcatctatgttactagatcgggaattcactggccgtcgttttacaacgt 
               
               
                   
               
               
                 cgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgcca 
               
               
                   
               
               
                 gctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatgg 
               
               
                   
               
               
                 cgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagc 
               
               
                   
               
               
                 tctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaa 
               
               
                   
               
               
                 cttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttga 
               
               
                   
               
               
                 cgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctat 
               
               
                   
               
               
                 ctcgggctattcttttgatttataagggattttgccgatttcggaaccaccatcaaacaggatt 
               
               
                   
               
               
                 ttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaa 
               
               
                   
               
               
                 gggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccccagtacattaaaaacgt 
               
               
                   
               
               
                 ccgcaatgtgttattaagttgtctaagcgtcaattt gtttacaccacaatatatcctgcca   
               
               
                   
               
               
                 (Underlined Seq: RD29A promoter; Bold: Anti-sense-AtFTA) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 48 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatcctc GCTCTTCCTCCATGCCCAATAGTTAGCTCTTACAGGATCTACACGACCAAGAATAGT   
               
               
                   
               
               
                 
                   ACACACCAAATTGGCCAAGTTAGTCTCTGGTTCTTCATTAGCTAGAGCTCTCACTGAGTCTTTA 
                 
               
               
                   
               
               
                 
                   TGCTCGTTGGTTGGTCTCAGTCCATCACATAGAAGATCCAAAAGGGTGCTCAGAGCGAATCCAT 
                 
               
               
                   
               
               
                 
                   GGAAGCAATCTGTGCGGGATAGAACATTCAAACAGACTGAGGAAACACTTGGATCACTAATCCA 
                 
               
               
                   
               
               
                 
                   GGATTCTTTGTCGTCTTTGTAAAGAGCTTTTAGGTATCGCCATGAGCTCTCGTTTGCAGGATTG 
                 
               
               
                   
               
               
                 
                   GTTAAAATGGCTTTGATTGTGTAGCTTACTTCAGATTCTCTCATGGCTTCTAGGCCTCCCAACA 
                 
               
               
                   
               
               
                 
                   AAGGAGATTGGGTGATGACATAATACCTCTGATTCCAGGCGGAATTGTTAAAGACGTCAGCTTC 
                 
               
               
                   
               
               
                   AAGGAGCTCGTGACAGTAATCGAGCTCATCTTCCCATCCTCCTAATGCCCG gaggatccccATC 
               
               
                   
               
               
                 TACCCGCTTCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCGAACAGTTCCTGATTAACCACA 
               
               
                   
               
               
                 AACCGTTCTACTTTACTGGCTTTGGTCGTCATGAAGATGCGGACTTGCGTGGCAAAGGATTCGA 
               
               
                   
               
               
                 TAACGTGCTGATGGTGCACGACCACGCATTAATGGACTGGATTGGGGCCAACTCCTACCGTACC 
               
               
                   
               
               
                 TCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGTGGTGATTG 
               
               
                   
               
               
                 ATGAAACTGCTGCTGTCGGCTTTTCGCTCTCTTTAGGCATTGGTTTCGAAGCGGGCAACAAGCC 
               
               
                   
               
               
                 GAAAGAACTGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAAGCGCACTTACAGGCGATT 
               
               
                   
               
               
                 AAAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGGTGATGTGGAGTATTGCCAACGAAC 
               
               
                   
               
               
                 CGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCCACTGGCGGAAGCAACGCGTAAACT 
               
               
                   
               
               
                 CGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCACACCGATACCATC 
               
               
                   
               
               
                 AGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATGGTATGTCCAAAGCGGCGATT 
               
               
                   
               
               
                 TGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGGCAGGAGAAACTGTACACCGA 
               
               
                   
               
               
                 CATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATCACCGCGTCTTTGATCGCGTC 
               
               
                   
               
               
                 AGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGCGACCTCGCAAGGCATATTGC 
               
               
                   
               
               
                 GCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCT 
               
               
                   
               
               
                 GCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATGA 
               
               
                   
               
               
                 ATCAACAACTCTCCTGGCGCACCATCGTCGGCTACAGCCTCGGGAATTGCTACCGAGCTC GCTC   
               
               
                   
               
               
                 
                   TTCCTCCATGCCCAATAGTTAGCTCTTACAGGATCTACACGACCAAGAATAGTACACACCAAAT 
                 
               
               
                   
               
               
                 
                   TGGCCAAGTTAGTCTCTGGTTCTTCATTAGCTAGAGCTCTCACTGAGTCTTTATGCTCGTTGGT 
                 
               
               
                   
               
               
                 
                   TGGTCTCAGTCCATCACATAGAAGATCCAAAAGGGTGCTCAGAGCGAATCCATGGAAGCAATCT 
                 
               
               
                   
               
               
                 
                   GTGCGGGATAGAACATTCAAACAGACTGAGGAAACACTTGGATCACTAATCCAGGATTCTTTGT 
                 
               
               
                   
               
               
                 
                   CGTCTTTGTAAAGAGCTTTTAGGTATCGCCATGAGCTCTCGTTTGCAGGATTGGTTAAAATGGC 
                 
               
               
                   
               
               
                 
                   TTTGATTGTGTAGCTTACTTCAGATTCTCTCATGGCTTCTAGGCCTCCCAACAAAGGAGATTGG 
                 
               
               
                   
               
               
                 
                   GTGATGACATAATACCTCTGATTCCAGGCGGAATTGTTAAAGACGTCAGCTTCAAGGAGCTCGT 
                 
               
               
                   
               
               
                   GACAGTAATCGAGCTCATCTTCCCATCCTCCTAATGCCCG ctcgaatttccccgatcgttcaaa 
               
               
                   
               
               
                 catttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataa 
               
               
                   
               
               
                 tttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagat 
               
               
                   
               
               
                 gggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcg 
               
               
                   
               
               
                 cgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcactggc 
               
               
                   
               
               
                 cgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagca 
               
               
                   
               
               
                 catccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagt 
               
               
                   
               
               
                 tgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccg 
               
               
                   
               
               
                 gctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggca 
               
               
                   
               
               
                 cctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacg 
               
               
                   
               
               
                 gtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaa 
               
               
                   
               
               
                 caacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggaacc 
               
               
                   
               
               
                 accatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctc 
               
               
                   
               
               
                 agggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccacccc 
               
               
                   
               
               
                 agtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttacaccaca   
               
               
                   
               
               
                 
                   atatatcctgcca 
                 
               
               
                   
               
               
                 (Underlined Seq: 35S promoter; Bold: AtFTA anti-sense sequence 
               
               
                 separated by GUS Seq.) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 49 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaagggtttgattacttctattggaaag gactctagaggatc 
               
               
                   
               
               
                 ctc GCTCTTCCTCCATGCCCAATAGTTAGCTCTTACAGGATCTACACGACCAAGAATAGTACAC   
               
               
                   
               
               
                 
                   ACCAAATTGGCCAAGTTAGTCTCTGGTTCTTCATTAGCTAGAGCTCTCACTGAGTCTTTATGCT 
                 
               
               
                   
               
               
                 
                   CGTTGGTTGGTCTCAGTCCATCACATAGAAGATCCAAAAGGGTGCTCAGAGCGAATCCATGGAA 
                 
               
               
                   
               
               
                 
                   GCAATCTGTGCGGGATAGAACATTCAAACAGACTGAGGAAACACTTGGATCACTAATCCAGGAT 
                 
               
               
                   
               
               
                 
                   TCTTTGTCGTCTTTGTAAAGAGCTTTTAGGTATCGCCATGAGCTCTCGTTTGCAGGATTGGTTA 
                 
               
               
                   
               
               
                 
                   AAATGGCTTTGATTGTGTAGCTTACTTCAGATTCTCTCATGGCTTCTAGGCCTCCCAACAAAGG 
                 
               
               
                   
               
               
                 
                   AGATTGGGTGATGACATAATACCTCTGATTCCAGGCGGAATTGTTAAAGACGTCAGCTTCAAGG 
                 
               
               
                   
               
               
                   AGCTCGTGACAGTAATCGAGCTCATCTTCCCATCCTCCTAATGCCCG gaggatccccATCTACC 
               
               
                   
               
               
                 CGCTTCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCGAACAGTTCCTGATTAACCACAAACC 
               
               
                   
               
               
                 GTTCTACTTTACTGGCTTTGGTCGTCATGAAGATGCGGACTTGCGTGGCAAAGGATTCGATAAC 
               
               
                   
               
               
                 GTGCTGATGGTGCACGACCACGCATTAATGGACTGGATTGGGGCCAACTCCTACCGTACCTCGC 
               
               
                   
               
               
                 ATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGTGGTGATTGATGA 
               
               
                   
               
               
                 AACTGCTGCTGTCGGCTTTTCGCTCTCTTTAGGCATTGGTTTCGAAGCGGGCAACAAGCCGAAA 
               
               
                   
               
               
                 GAACTGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAAGCGCACTTACAGGCGATTAAAG 
               
               
                   
               
               
                 AGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGGTGATGTGGAGTATTGCCAACGAACCGGA 
               
               
                   
               
               
                 TACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCCACTGGCGGAAGCAACGCGTAAACTCGAC 
               
               
                   
               
               
                 CCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCACACCGATACCATCAGCG 
               
               
                   
               
               
                 ATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATGGTATGTCCAAAGCGGCGATTTGGA 
               
               
                   
               
               
                 AACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGGCAGGAGAAACTGTACACCGACATG 
               
               
                   
               
               
                 TGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATCACCGCGTCTTTGATCGCGTCAGCG 
               
               
                   
               
               
                 CCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGCGACCTCGCAAGGCATATTGCGCGT 
               
               
                   
               
               
                 TGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTG 
               
               
                   
               
               
                 CAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATGAATCA 
               
               
                   
               
               
                 ACAACTCTCCTGGCGCACCATCGTCGGCTACAGCCTCGGGAATTGCTACCGAGCTC GCTCTTCC   
               
               
                   
               
               
                 
                   TCCATGCCCAATAGTTAGCTCTTACAGGATCTACACGACCAAGAATAGTACACACCAAATTGGC 
                 
               
               
                   
               
               
                 
                   CAAGTTAGTCTCTGGTTCTTCATTAGCTAGAGCTCTCACTGAGTCTTTATGCTCGTTGGTTGGT 
                 
               
               
                   
               
               
                 
                   CTCAGTCCATCACATAGAAGATCCAAAAGGGTGCTCAGAGCGAATCCATGGAAGCAATCTGTGC 
                 
               
               
                   
               
               
                 
                   GGGATAGAACATTCAAACAGACTGAGGAAACACTTGGATCACTAATCCAGGATTCTTTGTCGTC 
                 
               
               
                   
               
               
                 
                   TTTGTAAAGAGCTTTTAGGTATCGCCATGAGCTCTCGTTTGCAGGATTGGTTAAAATGGCTTTG 
                 
               
               
                   
               
               
                 
                   ATTGTGTAGCTTACTTCAGATTCTCTCATGGCTTCTAGGCCTCCCAACAAAGGAGATTGGGTGA 
                 
               
               
                   
               
               
                 
                   TGACATAATACCTCTGATTCCAGGCGGAATTGTTAAAGACGTCAGCTTCAAGGAGCTCGTGACA 
                 
               
               
                   
               
               
                   GTAATCGAGCTCATCTTCCCATCCTCCTAATGCCCG ctcgaatttccccgatcgttcaaacatt 
               
               
                   
               
               
                 tggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttc 
               
               
                   
               
               
                 tgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggt 
               
               
                   
               
               
                 ttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgca 
               
               
                   
               
               
                 aactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcactggccgtc 
               
               
                   
               
               
                 gttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatc 
               
               
                   
               
               
                 cccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcg 
               
               
                   
               
               
                 cagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctt 
               
               
                   
               
               
                 tccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctc 
               
               
                   
               
               
                 gaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggttt 
               
               
                   
               
               
                 ttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaac 
               
               
                   
               
               
                 actcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggaaccacca 
               
               
                   
               
               
                 tcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcaggg 
               
               
                   
               
               
                 ccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccccagta 
               
               
                   
               
               
                 cattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttacaccacaatat   
               
               
                   
               
               
                 
                   atcctgcca 
                 
               
               
                   
               
               
                 (Underlined Seq: RD29A promoter; Bold: AtFTA anti-sense sequence, 
               
               
                 separated by GUS Seq.) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 50 
                   
               
            
           
           
               
               
            
               
                 GAATTC AAATTTTTCGCCAGTTCTAAATATCCGGAAACCTCTTGGGATGCCATTGCCCATCTAT   
                   
               
               
                   
               
               
                 
                   CTGTAATTTATTGACGAAATAGACGAAAAGGAAGGTGGCTCCTATAAAGCACATCATTGCGATA 
                 
               
               
                   
               
               
                 
                   ACAGAAAGGCCATTGTTGAAGATACCTCTGCTGACATTGGTCCCCAAGTGGAAGCACCACCCCA 
                 
               
               
                   
               
               
                 
                   TGAGGAGCACCGTGGAGTAAGAAGACGTTCGAGCCACGTCGAAAAAGCAAGTGTGTTGATGTAG 
                 
               
               
                   
               
               
                 
                   TATCTCCATTGACGTAAGGGATGACGCACAATCCAACTATCCATCGCAAGACCATTGCTCTATA 
                 
               
               
                   
               
               
                   TAAGAAAGTTAATATCATTTCGAGTGGCCACGCT GAGCTC AGGAAGTCTGCTCTTGCGCCAAAT   
               
               
                   
               
               
                 
                   CCAATAGTTGGTTCTAATTGGATCAACTTGTTTTAGGATAGAACAAATATTTCGTGCTATATTT 
                 
               
               
                   
               
               
                 
                   AAATTTTGTTGTTCCCCTTTCTCATCATCATCTAAATCTTGTTTATCCATATCTGCGGTCTTTA 
                 
               
               
                   
               
               
                 
                   AGGCGTCAATGGCATCTCTAATGTCTTCATTTGGTTGATAACCAAAGCATATAAGATCTAAAAT 
                 
               
               
                   
               
               
                 
                   AGTGCTAAGAGCAAACACGTAGTTGCTCTTAGTTCTCAAAATCTTTAAGCATACTGAAGAAACT 
                 
               
               
                   
               
               
                 
                   TGAGGATCATTTACCCATGAAGTAGTTTCACCTTTATAAAGTCCTCGTAGATATCTCCACGAGC 
                 
               
               
                   
               
               
                 
                   TTTCATTTTCAGGGTAGGCTATAATGGCTTCGATGGTGTAAAGCACTTCAGACTCTCTCATAGC 
                 
               
               
                   
               
               
                 
                   TTTTAGGCCCCCCAAGAAAGGAGACCTTGTTATGACAAAATATCTCTGATTCCAAGCAGAATTG 
                 
               
               
                   
               
               
                 
                   TTAAAAATGTCTTCTTTAAGTAGTTCTGTGCAATAATTAAGTTCATCTTCCCATCCTCCTAGTG 
                 
               
               
                   
               
               
                 
                   TTTGAAGAGCCCACTGTCTATGAGACCATGCATGATAATGTTTGGCATCAACGGACAGTATCTT 
                 
               
               
                   
               
               
                   TTTGGTGAACTCGAGCT gagctcgaatttccccgatcgttcaaacatttggcaataaagtttct 
               
               
                   
               
               
                 taagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaa 
               
               
                   
               
               
                 gcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtc 
               
               
                   
               
               
                 ccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattat 
               
               
                   
               
               
                 cgcgcgcggtgtcatctatgttactagatcgggaattc 
               
               
                   
               
               
                 (Underlined MuA Promoter; Bold:  Glycine max  anti-FTA; 
               
               
                 lower case: NOS terminater Seq.) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 51 
                   
               
            
           
           
               
               
            
               
                 
                   GGAGCCATAGATGCAATTCAATCAAACTGAAATTTCTGCAAGAATCTCAAACACGGAGATCTCA 
                 
                   
               
               
                   
               
               
                 
                   AAGTTTGAAAGAAAATTTATTTCTTCGACTCAAAACAAACTTACGAAATTTAGGTAGAACTTAT 
                 
               
               
                   
               
               
                 
                   ATACATTATATTGTAATTTTTTGTAACAAAATGTTTTTATTATTATTATAGAATTTTACTGGTT 
                 
               
               
                   
               
               
                 
                   AAATTAAAAATGAATAGAAAAGGTGAATTAAGAGGAGAGAGGAGGTAAACATTTTCTTCTATTT 
                 
               
               
                   
               
               
                 
                   TTTCATATTTTCAGGATAAATTATTGTAAAAGTTTACAAGATTTCCATTTGACTAGTGTAAATG 
                 
               
               
                   
               
               
                 
                   AGGAATATTCTCTAGTAAGATCATTATTTCATCTACTTCTTTTATCTTCTACCAGTAGAGGAAT 
                 
               
               
                   
               
               
                 
                   AAACAATATTTAGCTCCTTTGTAAATACAAATTAATTTTCCTTCTTGACATCATTCAATTTTAA 
                 
               
               
                   
               
               
                 
                   TTTTACGTATAAAATAAAAGATCATACCTATTAGAACGATTAAGGAGAAATACAATTCGAATGA 
                 
               
               
                   
               
               
                 
                   GAAGGATGTGCCGTTTGTTATAATAAACAGCCACACGACGTAAACGTAAAATGACCACATGATG 
                 
               
               
                   
               
               
                 
                   GGCCAATAGACATGGACCGACTACTAATAATAGTAAGTTACATTTTAGGATGGAATAAATATCA 
                 
               
               
                   
               
               
                 
                   TACCGACATCAGTTTTGAAAGAAAAGGGAAAAAAAGAAAAAATAAATAAAAGATATACTACCGA 
                 
               
               
                   
               
               
                 
                   CATGAGTTCCAAAAAGCAAAAAAAAAGATCAAGCCGACACAGACACGCGTAGAGAGCAAAATGA 
                 
               
               
                   
               
               
                 
                   CTTTGACGTCACACCACGAAAACAGACGCTTCATACGTGTCCCTTTATCTCTCTCAGTCTCTCT 
                 
               
               
                   
               
               
                 
                   ATAAACTTAGTGAGACCCTCCTCTGTTTTACTCACAAATATGCAAACTAGAAAACAATCATCAG 
                 
               
               
                   
               
               
                 
                   GAATAAAGGGTTTGATTACTTCTATTGGAAA 
                   GAGGAAGTCTGCTCTTGCGCCAAATCCAATAGT 
                 
               
               
                   
               
               
                 
                   TGGTTCTAATTGGATCAACTTGTTTTAGGATAGAACAAATATTTCGTGCTATATTTAAATTTTG 
                 
               
               
                   
               
               
                 
                   TTGTTCCCCTTTCTCATCATCATCTAAATCTTGTTTATCCATATCTGCGGTCTTTAAGGCGTCA 
                 
               
               
                   
               
               
                 
                   ATGGCATCTCTAATGTCTTCATTTGGTTGATAACCAAAGCATATAAGATCTAAAATAGTGCTAA 
                 
               
               
                   
               
               
                 
                   GAGCAAACACGTAGTTGCTCTTAGTTCTCAAAATCTTTAAGCATACTGAAGAAACTTGAGGATC 
                 
               
               
                   
               
               
                 
                   ATTTACCCATGAAGTAGTTTCACCTTTATAAAGTCCTCGTAGATATCTCCACGAGCTTTCATTT 
                 
               
               
                   
               
               
                 
                   TCAGGGTAGGCTATAATGGCTTCGATGGTGTAAAGCACTTCAGACTCTCTCATAGCTTTTAGGC 
                 
               
               
                   
               
               
                 
                   CCCCCAAGAAAGGAGACCTTGTTATGACAAAATATCTCTGATTCCAAGCAGAATTGTTAAAAAT 
                 
               
               
                   
               
               
                 
                   GTCTTCTTTAAGTAGTTCTGTGCAATAATTAAGTTCATCTTCCCATCCTCCTAGTGTTTGAAGA 
                 
               
               
                   
               
               
                 
                   GCCCACTGTCTATGAGACCATGCATGATAATGTTTGGCATCAACGGACAGTATCTTTTTGGTGA 
                 
               
               
                   
               
               
                   ACTCGAGCT gagctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattg 
               
               
                   
               
               
                 aatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaa 
               
               
                   
               
               
                 taattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaatt 
               
               
                   
               
               
                 atacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcg 
               
               
                   
               
               
                 gtgtcatctatgttactagatcgggaattc 
               
               
                   
               
               
                 (Underlined RD29A Promoter; Bold:  Glycine max  anti- 
               
               
                   Glycine max  FTA; lower case: NOS terminater Seq.) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 52 
                   
               
            
           
           
               
               
            
               
                 GAATTC AAATTTTTCGCCAGTTCTAAATATCCGGAAACCTCTTGGGATGCCATTGCCCATCTAT   
                   
               
               
                   
               
               
                 
                   CTGTAATTTATTGACGAAATAGACGAAAAGGAAGGTGGCTCCTATAAAGCACATCATTGCGATA 
                 
               
               
                   
               
               
                 
                   ACAGAAAGGCCATTGTTGAAGATACCTCTGCTGACATTGGTCCCCAAGTGGAAGCACCACCCCA 
                 
               
               
                   
               
               
                 
                   TGAGGAGCACCGTGGAGTAAGAAGACGTTCGAGCCACGTCGAAAAAGCAAGTGTGTTGATGTAG 
                 
               
               
                   
               
               
                 
                   TATCTCCATTGACGTAAGGGATGACGCACAATCCAACTATCCATCGCAAGACCATTGCTCTATA 
                 
               
               
                   
               
               
                   TAAGAAAGTTAATATCATTTCGAGTGGCCACGCT GAGCTC AGGAAGTCTGCTCTTGCGCCAAAT   
               
               
                   
               
               
                 
                   CCAATAGTTGGTTCTAATTGGATCAACTTGTTTTAGGATAGAACAAATATTTCGTGCTATATTT 
                 
               
               
                   
               
               
                 
                   AAATTTTGTTGTTCCCCTTTCTCATCATCATCTAAATCTTGTTTATCCATATCTGCGGTCTTTA 
                 
               
               
                   
               
               
                 
                   AGGCGTCAATGGCATCTCTAATGTCTTCATTTGGTTGATAACCAAAGCATATAAGATCTAAAAT 
                 
               
               
                   
               
               
                 
                   AGTGCTAAGAGCAAACACGTAGTTGCTCTTAGTTCTCAAAATCTTTAAGCATACTGAAGAAACT 
                 
               
               
                   
               
               
                 
                   TGAGGATCATTTACCCATGAAGTAGTTTCACCTTTATAAAGTCCTCGTAGATATCTCCACGAGC 
                 
               
               
                   
               
               
                 
                   TTTCATTTTCAGGGTAGGCTATAATGGCTTCGATGGTGTAAAGCACTTCAGACTCTCTCATAGC 
                 
               
               
                   
               
               
                 
                   TTTTAGGCCCCCCAAGAAAGGAGACCTTGTTATGACAAAATATCTCTGATTCCAAGCAGAATTG 
                 
               
               
                   
               
               
                 
                   TTAAAAATGTCTTCTTTAAGTAGTTCTGTGCAATAATTAAGTTCATCTTCCCATCCTCCTAGTG 
                 
               
               
                   
               
               
                 
                   TTTGAAGAGCCCACTGTCTATGAGACCATGCATGATAATGTTTGGCATCAACGGACAGTATCTT 
                 
               
               
                   
               
               
                 
                   TTTGGTGAACTCGAGCT 
                   TAAAGGTGAAACTACTTCATGGGTAAATGATCCTCAAGTTTCTTCAG 
                 
               
               
                   
               
               
                 
                   TATGCTTAAAGATTTTGAGAACTAAGAGCAACTACGTGTTTGCTCTTAGCACTATTTTAGATCT 
                 
               
               
                   
               
               
                 
                   TATATGCTTTGGTTATCAACCAAATGAAGACATTAGAGATGCCATTGACGCCTTAAAGACCGCA 
                 
               
               
                   
               
               
                 
                   GATATGGATAAACAAGATTTAGATGATGATGAGAAAGGGGAACAACAAAATTTAAATATAGCAC 
                 
               
               
                   
               
               
                 
                   GAAATATTTGTTCTATCCTAAAACAAGTTGATCCAATTAGAACCAACTATTGGATTTGGCGCAA 
                 
               
               
                   
               
               
                   GAGCAGACTTCCT   gagctc gaatttccccgatcgttcaaacatttggcaataaagtttcttaag 
               
               
                   
               
               
                 attgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcat 
               
               
                   
               
               
                 gtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgc 
               
               
                   
               
               
                 aattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcg 
               
               
                   
               
               
                 cgcggtgtcatctatgttactagatcgggaattc 
               
               
                   
               
               
                 (Underlined:  Glycine max  FTA Anti-Sense section; 
               
               
                 Bold: MuA Promoter; Italics:  Glycine max  FTA Sense section; 
               
               
                 lower case: NOS terminater Seq.) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 53 
                   
               
            
           
           
               
               
            
               
                 
                   ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatctcaaacacggagatctca 
                 
                   
               
               
                   
               
               
                 
                   aagtttgaaagaaaatttatttcttcgactcaaaacaaacttacgaaatttaggtagaacttat 
                 
               
               
                   
               
               
                 
                   atacattatattgtaattttttgtaacaaaatgtttttattattattatagaattttactggtt 
                 
               
               
                   
               
               
                 
                   aaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggtaaacattttcttctattt 
                 
               
               
                   
               
               
                 
                   tttcatattttcaggataaattattgtaaaagtttacaagatttccatttgactagtgtaaatg 
                 
               
               
                   
               
               
                 
                   aggaatattctctagtaagatcattatttcatctacttcttttatcttctaccagtagaggaat 
                 
               
               
                   
               
               
                 
                   aaacaatatttagctcctttgtaaatacaaattaattttccttcttgacatcattcaattttaa 
                 
               
               
                   
               
               
                 
                   ttttacgtataaaataaaagatcatacctattagaacgattaaggagaaatacaattcgaatga 
                 
               
               
                   
               
               
                 
                   gaaggatgtgccgtttgttataataaacagccacacgacgtaaacgtaaaatgaccacatgatg 
                 
               
               
                   
               
               
                 
                   ggccaatagacatggaccgactactaataatagtaagttacattttaggatggaataaatatca 
                 
               
               
                   
               
               
                 
                   taccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaataaaagatatactaccga 
                 
               
               
                   
               
               
                 
                   catgagttccaaaaagcaaaaaaaaagatcaagccgacacagacacgcgtagagagcaaaatga 
                 
               
               
                   
               
               
                 
                   ctttgacgtcacaccacgaaaacagacgcttcatacgtgtccctttatctctctcagtctctct 
                 
               
               
                   
               
               
                 
                   ataaacttagtgagaccctcctctgttttactcacaaatatgcaaactagaaaacaatcatcag 
                 
               
               
                   
               
               
                 
                   gaataaagggtttgattacttctattggaaag 
                   AGGAAGTCTGCTCTTGCGCCAAATCCAATAGT 
                 
               
               
                   
               
               
                 
                   TGGTTCTAATTGGATCAACTTGTTTTAGGATAGAACAAATATTTCGTGCTATATTTAAATTTTG 
                 
               
               
                   
               
               
                 
                   TTGTTCCCCTTTCTCATCATCATCTAAATCTTGTTTATCCATATCTGCGGTCTTTAAGGCGTCA 
                 
               
               
                   
               
               
                 
                   ATGGCATCTCTAATGTCTTCATTTGGTTGATAACCAAAGCATATAAGATCTAAAATAGTGCTAA 
                 
               
               
                   
               
               
                 
                   GAGCAAACACGTAGTTGCTCTTAGTTCTCAAAATCTTTAAGCATACTGAAGAAACTTGAGGATC 
                 
               
               
                   
               
               
                 
                   ATTTACCCATGAAGTAGTTTCACCTTTATAAAGTCCTCGTAGATATCTCCACGAGCTTTCATTT 
                 
               
               
                   
               
               
                 
                   TCAGGGTAGGCTATAATGGCTTCGATGGTGTAAAGCACTTCAGACTCTCTCATAGCTTTTAGGC 
                 
               
               
                   
               
               
                 
                   CCCCCAAGAAAGGAGACCTTGTTATGACAAAATATCTCTGATTCCAAGCAGAATTGTTAAAAAT 
                 
               
               
                   
               
               
                 
                   GTCTTCTTTAAGTAGTTCTGTGCAATAATTAAGTTCATCTTCCCATCCTCCTAGTGTTTGAAGA 
                 
               
               
                   
               
               
                 
                   GCCCACTGTCTATGAGACCATGCATGATAATGTTTGGCATCAACGGACAGTATCTTTTTGGTGA 
                 
               
               
                   
               
               
                   ACTCGAGCT TAAAGGTGAAACTACTTCATGGGTAAATGATCCTCAAGTTTCTTCAGTATGCTTA 
               
               
                   
               
               
                 AAGATTTTGAGAACTAAGAGCAACTACGTGTTTGCTCTTAGCACTATTTTAGATCTTATATGCT 
               
               
                   
               
               
                 TTGGTTATCAACCAAATGAAGACATTAGAGATGCCATTGACGCCTTAAAGACCGCAGATATGGA 
               
               
                   
               
               
                 TAAACAAGATTTAGATGATGATGAGAAAGGGGAACAACAAAATTTAAATATAGCACGAAATATT 
               
               
                   
               
               
                 TGTTCTATCCTAAAACAAGTTGATCCAATTAGAACCAACTATTGGATTTGGCGCAAGAGCAGAC 
               
               
                   
               
               
                 TTCCT gagctc gaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatc 
               
               
                   
               
               
                 ctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataat 
               
               
                   
               
               
                 taacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatac 
               
               
                   
               
               
                 atttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgt 
               
               
                   
               
               
                 catctatgttactagatcgggaattc 
               
               
                   
               
               
                 (Bold lower case: RD29A Promoter; Underline, Upper case: 
               
               
                 Antisense GmFTA; Upper case: Sense GmFTA; lower case: 
               
               
                 NOS terminater) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 54 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                 
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg 
                   gactctagag 
                 
               
               
                   
               
               
                 
                   gatccgtccggaattcccgggtcgacccacgcgtccgggagattcagcgagataagcaattgga 
                 
               
               
                   
               
               
                 
                   ttatctgatgaaaggcttaaggcagcttggtccgcagttttcttccttagatgctaatcgacct 
                 
               
               
                   
               
               
                 
                   tggctttgttactggattcttcattcaatagctttgcttggggagactgtggatgatgaattag 
                 
               
               
                   
               
               
                 
                   aaagcaatgccattgacttccttggacgctgccagggctctgaaggtggatacggtggtggtcc 
                 
               
               
                   
               
               
                 
                   tggccaacttccacatcttgcaactacttatgctgcagtgaatgcacttgttactttaggaggt 
                 
               
               
                   
               
               
                 
                   gacaaagccctttcttcaattaatagagaaaaaatgtcttgttttttaagacggatgaaggata 
                 
               
               
                   
               
               
                 
                   caagtggaggtttcaggatgcatgatatgggagaaatggatgttcgtgcatgctacactgcaat 
                 
               
               
                   
               
               
                 
                   ttcggttgcaagcatcctaaatattatggatgatgaactcacccagggcctaggagattacatc 
                 
               
               
                   
               
               
                 
                   ttgagttgccaaacttatgaaggtggcattggaggggaacctggctccgaagctcacggtgggt 
                 
               
               
                   
               
               
                 
                   atacctactgtggtttggctgctatgattttaatcaatgaggtcgaccgtttgaatttggattc 
                 
               
               
                   
               
               
                 
                   attaatgaattgggctgtacatcgacaaggagtagaaatgggatttcaaggtaggacgaacaaa 
                 
               
               
                   
               
               
                 
                   ttggtcgatggttgctacacattttggcaggcagccccttgtgttctactacaaagattatatt 
                 
               
               
                   
               
               
                 
                   caaccaatgatcatgacgttcatggatcatcacatatatcagaagggacaaatgaagaacatca 
                 
               
               
                   
               
               
                 
                   tgctcatgatgaagatgaccttgaagacagtgatgatgatgatgattctgatgaggacaacgat 
                 
               
               
                   
               
               
                 
                   gaagattcagtgaatggtcacagaatccatcatacatccacctacattaacaggagaatgcaac 
                 
               
               
                   
               
               
                 
                   tggtttttgatagcctcggcttgcagagatatgtactcttgtgctctaagatccctgacggtgg 
                 
               
               
                   
               
               
                 
                   attcagagacaagccgaggaaaccccgtgacttctaccacacatgttactgcctgagcggcttg 
                 
               
               
                   
               
               
                 
                   tctgtggctcagcacgcttggttaaaagacgaggacactcctcctttgactcgcgacattatgg 
                 
               
               
                   
               
               
                 
                   gtggctactcgaatctccttgaacctgttcaacttcttcacaacattgtcatggatcagtataa 
                 
               
               
                   
               
               
                   tgaagctatcgagttcttctttaaagcagcatga ctcgaatttccccgatcgttcaaacatttg 
               
               
                   
               
               
                 gcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctg 
               
               
                   
               
               
                 ttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggttt 
               
               
                   
               
               
                 ttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaa 
               
               
                   
               
               
                 ctaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcactggccgtcgt 
               
               
                   
               
               
                 tttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccc 
               
               
                   
               
               
                 cctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgca 
               
               
                   
               
               
                 gcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttc 
               
               
                   
               
               
                 cccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcga 
               
               
                   
               
               
                 ccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggttttt 
               
               
                   
               
               
                 cgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacac 
               
               
                   
               
               
                 tcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggaaccaccatc 
               
               
                   
               
               
                 aaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggcc 
               
               
                   
               
               
                 aggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccccagtaca 
               
               
                   
               
               
                 ttaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttacaccacaatatat   
               
               
                   
               
               
                 
                   cctgcca 
                 
               
               
                   
               
               
                 (Underline: 35S promoter; Bold: anti-AtFTB) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 55 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                 
                   ctagaaaacaatcatcaggaataaagggtttgattacttctattggaaag 
                   gactctagaggatc 
                 
               
               
                   
               
               
                 
                   cgtccggaattcccgggtcgacccacgcgtccgggagattcagcgagataagcaattggattat 
                 
               
               
                   
               
               
                 
                   ctgatgaaaggcttaaggcagcttggtccgcagttttcttccttagatgctaatcgaccttggc 
                 
               
               
                   
               
               
                 
                   tttgttactggattcttcattcaatagctttgcttggggagactgtggatgatgaattagaaag 
                 
               
               
                   
               
               
                 
                   caatgccattgacttccttggacgctgccagggctctgaaggtggatacggtggtggtcctggc 
                 
               
               
                   
               
               
                 
                   caacttccacatcttgcaactacttatgctgcagtgaatgcacttgttactttaggaggtgaca 
                 
               
               
                   
               
               
                 
                   aagccctttcttcaattaatagagaaaaaatgtcttgttttttaagacggatgaaggatacaag 
                 
               
               
                   
               
               
                 
                   tggaggtttcaggatgcatgatatgggagaaatggatgttcgtgcatgctacactgcaatttcg 
                 
               
               
                   
               
               
                 
                   gttgcaagcatcctaaatattatggatgatgaactcacccagggcctaggagattacatcttga 
                 
               
               
                   
               
               
                 
                   gttgccaaacttatgaaggtggcattggaggggaacctggctccgaagctcacggtgggtatac 
                 
               
               
                   
               
               
                 
                   ctactgtggtttggctgctatgattttaatcaatgaggtcgaccgtttgaatttggattcatta 
                 
               
               
                   
               
               
                 
                   atgaattgggctgtacatcgacaaggagtagaaatgggatttcaaggtaggacgaacaaattgg 
                 
               
               
                   
               
               
                 
                   tcgatggttgctacacattttggcaggcagccccttgtgttctactacaaagattatattcaac 
                 
               
               
                   
               
               
                 
                   caatgatcatgacgttcatggatcatcacatatatcagaagggacaaatgaagaacatcatgct 
                 
               
               
                   
               
               
                 
                   catgatgaagatgaccttgaagacagtgatgatgatgatgattctgatgaggacaacgatgaag 
                 
               
               
                   
               
               
                 
                   attcagtgaatggtcacagaatccatcatacatccacctacattaacaggagaatgcaactggt 
                 
               
               
                   
               
               
                 
                   ttttgatagcctcggcttgcagagatatgtactcttgtgctctaagatccctgacggtggattc 
                 
               
               
                   
               
               
                 
                   agagacaagccgaggaaaccccgtgacttctaccacacatgttactgcctgagcggcttgtctg 
                 
               
               
                   
               
               
                 
                   tggctcagcacgcttggttaaaagacgaggacactcctcctttgactcgcgacattatgggtgg 
                 
               
               
                   
               
               
                 
                   ctactcgaatctccttgaacctgttcaacttcttcacaacattgtcatggatcagtataatgaa 
                 
               
               
                   
               
               
                   gctatcgagttcttctttaaagcagcatga ctcgaatttccccgatcgttcaaacatttggcaa 
               
               
                   
               
               
                 taaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttga 
               
               
                   
               
               
                 attacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttat 
               
               
                   
               
               
                 gattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactag 
               
               
                   
               
               
                 gataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcactggccgtcgtttta 
               
               
                   
               
               
                 caacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctt 
               
               
                   
               
               
                 tcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcct 
               
               
                   
               
               
                 gaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccg 
               
               
                   
               
               
                 tcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgacccc 
               
               
                   
               
               
                 aaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgcc 
               
               
                   
               
               
                 ctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaa 
               
               
                   
               
               
                 ccctatctcgggctattcttttgatttataagggattttgccgatttcggaaccaccatcaaac 
               
               
                   
               
               
                 aggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggc 
               
               
                   
               
               
                 ggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccccagtacattaa 
               
               
                   
               
               
                 aaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttacaccacaatatatcctg   
               
               
                   
               
               
                 
                   cca 
                 
               
               
                   
               
               
                 (Underline: RD29A Promoter; Bold: anti-AtFTB) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 56 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatcctc CTCCTAGGCCCTGGGTGAGTTCATCATCCATAATATTTAGGATGCTTGCAACCGAAA   
               
               
                   
               
               
                 
                   TTGCAGTGTAGCATGCACGAACATCCATTTCTCCCATATCATGCATCCTGAAACCTCCACTTGT 
                 
               
               
                   
               
               
                 
                   ATCCTTCATCCGTCTTAAAAAACAAGACATTTTTTCTCTATTAATTGAAGAAAGGGCTTTGTCA 
                 
               
               
                   
               
               
                 
                   CCTCCTAAAGTAACAAGTGCATTCACTGCAGCATAAGTAGTTGCAAGATGTGGAAGTTGGCCAG 
                 
               
               
                   
               
               
                 
                   GACCACCACCGTATCCACCTTCAGAGCCCTGGCAGCGTCCAAGGAAGTCAATGGCATTGCTTTC 
                 
               
               
                   
               
               
                 
                   TAATTCATCATCCACAGTCTCCCCAAGCAAAGCTATTGAATGAAGAATCCAGTAACAAAGCCAA 
                 
               
               
                   
               
               
                 
                   GGTCGATTAGCATCTAAGGAAGAAAACTGCGGACCAAGCTGCCTTAAGCCTTTCATCAGATAAT 
                 
               
               
                   
               
               
                   CCAATTGCTTATCTCGCTGAATCTCCCGGACGCGTGGGTCGACCCGGGAATTCCGGAC gaggat 
               
               
                   
               
               
                 ccccATCTACCCGCTTCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCGAACAGTTCCTGATT 
               
               
                   
               
               
                 AACCACAAACCGTTCTACTTTACTGGCTTTGGTCGTCATGAAGATGCGGACTTGCGTGGCAAAG 
               
               
                   
               
               
                 GATTCGATAACGTGCTGATGGTGCACGACCACGCATTAATGGACTGGATTGGGGCCAACTCCTA 
               
               
                   
               
               
                 CCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGTG 
               
               
                   
               
               
                 GTGATTGATGAAACTGCTGCTGTCGGCTTTTCGCTCTCTTTAGGCATTGGTTTCGAAGCGGGCA 
               
               
                   
               
               
                 ACAAGCCGAAAGAACTGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAAGCGCACTTACA 
               
               
                   
               
               
                 GGCGATTAAAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGGTGATGTGGAGTATTGCC 
               
               
                   
               
               
                 AACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCCACTGGCGGAAGCAACGC 
               
               
                   
               
               
                 GTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCACACCGA 
               
               
                   
               
               
                 TACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATGGTATGTCCAAAGC 
               
               
                   
               
               
                 GGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGGCAGGAGAAACTGT 
               
               
                   
               
               
                 ACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATCACCGCGTCTTTGA 
               
               
                   
               
               
                 TCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGCGACCTCGCAAGGC 
               
               
                   
               
               
                 ATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCGCAAACCGAAGTCGGCGG 
               
               
                   
               
               
                 CTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAA 
               
               
                   
               
               
                 ACAATGAATCAACAACTCTCCTGGCGCACCATCGTCGGCTACAGCCTCGGGAATTGCTACCGAG 
               
               
                   
               
               
                 CTC   gtccggaattcccgggtcgacccacgcgtccgggagattcagcgagataagcaattggatt     
               
               
                   
               
               
                 
                   
                     atctgatgaaaggcttaaggcagcttggtccgcagttttcttccttagatgctaatcgaccttg 
                   
                 
               
               
                   
               
               
                 
                   
                     gctttgttactggattcttcattcaatagctttgcttggggagactgtggatgatgaattagaa 
                   
                 
               
               
                   
               
               
                 
                   
                     agcaatgccattgacttccttggacgctgccagggctctgaaggtggatacggtggtggtcctg 
                   
                 
               
               
                   
               
               
                 
                   
                     gccaacttccacatcttgcaactacttatgctgcagtgaatgcacttgttactttaggaggtga 
                   
                 
               
               
                   
               
               
                 
                   
                     caaagccctttcttcaattaatagagaaaaaatgtcttgttttttaagacggatgaaggataca 
                   
                 
               
               
                   
               
               
                 
                   
                     agtggaggtttcaggatgcatgatatgggagaaatggatgttcgtgcatgctacactgcaattt 
                   
                 
               
               
                   
               
               
                     cggttgcaagcatcctaaatattatggatgatgaactcacccagggcctaggag   ctcgaatttc 
               
               
                   
               
               
                 cccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcga 
               
               
                   
               
               
                 tgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgac 
               
               
                   
               
               
                 gttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaa 
               
               
                   
               
               
                 aacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatc 
               
               
                   
               
               
                 gggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaactta 
               
               
                   
               
               
                 atcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcg 
               
               
                   
               
               
                 cccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttct 
               
               
                   
               
               
                 cgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgattt 
               
               
                   
               
               
                 agtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccat 
               
               
                   
               
               
                 cgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactctt 
               
               
                   
               
               
                 gttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttg 
               
               
                   
               
               
                 ccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgctt 
               
               
                   
               
               
                 gctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaa 
               
               
                   
               
               
                 agaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaat 
               
               
                   
               
               
                 tt gtttacaccacaatatatcctgcca   
               
               
                   
               
               
                 (Underline: 35S promoter; Bold uppercase: antisense AtFTB; Lower 
               
               
                 case Bold: sense AtFTB) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 57 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaagggtttgattacttctattggaaag gactctagaggatc 
               
               
                   
               
               
                 ctc CTCCTAGGCCCTGGGTGAGTTCATCATCCATAATATTTAGGATGCTTGCAACCGAAATTGC   
               
               
                   
               
               
                 
                   AGTGTAGCATGCACGAACATCCATTTCTCCCATATCATGCATCCTGAAACCTCCACTTGTATCC 
                 
               
               
                   
               
               
                 
                   TTCATCCGTCTTAAAAAACAAGACATTTTTTCTCTATTAATTGAAGAAAGGGCTTTGTCACCTC 
                 
               
               
                   
               
               
                 
                   CTAAAGTAACAAGTGCATTCACTGCAGCATAAGTAGTTGCAAGATGTGGAAGTTGGCCAGGACC 
                 
               
               
                   
               
               
                 
                   ACCACCGTATCCACCTTCAGAGCCCTGGCAGCGTCCAAGGAAGTCAATGGCATTGCTTTCTAAT 
                 
               
               
                   
               
               
                 
                   TCATCATCCACAGTCTCCCCAAGCAAAGCTATTGAATGAAGAATCCAGTAACAAAGCCAAGGTC 
                 
               
               
                   
               
               
                 
                   GATTAGCATCTAAGGAAGAAAACTGCGGACCAAGCTGCCTTAAGCCTTTCATCAGATAATCCAA 
                 
               
               
                   
               
               
                   TTGCTTATCTCGCTGAATCTCCCGGACGCGTGGGTCGACCCGGGAATTCCGGAC gaggatcccc 
               
               
                   
               
               
                 ATCTACCCGCTTCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCGAACAGTTCCTGATTAACC 
               
               
                   
               
               
                 ACAAACCGTTCTACTTTACTGGCTTTGGTCGTCATGAAGATGCGGACTTGCGTGGCAAAGGATT 
               
               
                   
               
               
                 CGATAACGTGCTGATGGTGCACGACCACGCATTAATGGACTGGATTGGGGCCAACTCCTACCGT 
               
               
                   
               
               
                 ACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGTGGTGA 
               
               
                   
               
               
                 TTGATGAAACTGCTGCTGTCGGCTTTTCGCTCTCTTTAGGCATTGGTTTCGAAGCGGGCAACAA 
               
               
                   
               
               
                 GCCGAAAGAACTGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAAGCGCACTTACAGGCG 
               
               
                   
               
               
                 ATTAAAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGGTGATGTGGAGTATTGCCAACG 
               
               
                   
               
               
                 AACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCCACTGGCGGAAGCAACGCGTAA 
               
               
                   
               
               
                 ACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCACACCGATACC 
               
               
                   
               
               
                 ATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATGGTATGTCCAAAGCGGCG 
               
               
                   
               
               
                 ATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGGCAGGAGAAACTGTACAC 
               
               
                   
               
               
                 CGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATCACCGCGTCTTTGATCGC 
               
               
                   
               
               
                 GTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGCGACCTCGCAAGGCATAT 
               
               
                   
               
               
                 TGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCGCAAACCGAAGTCGGCGGCTTT 
               
               
                   
               
               
                 TCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAA 
               
               
                   
               
               
                 TGAATCAACAACTCTCCTGGCGCACCATCGTCGGCTACAGCCTCGGGAATTGCTACCGAGCTC   g     
               
               
                   
               
               
                 
                   
                     tccggaattcccgggtcgacccacgcgtccgggagattcagcgagataagcaattggattatct 
                   
                 
               
               
                   
               
               
                 
                   
                     gatgaaaggcttaaggcagcttggtccgcagttttcttccttagatgctaatcgaccttggctt 
                   
                 
               
               
                   
               
               
                 
                   
                     tgttactggattcttcattcaatagctttgcttggggagactgtggatgatgaattagaaagca 
                   
                 
               
               
                   
               
               
                 
                   
                     atgccattgacttccttggacgctgccagggctctgaaggtggatacggtggtggtcctggcca 
                   
                 
               
               
                   
               
               
                 
                   
                     acttccacatcttgcaactacttatgctgcagtgaatgcacttgttactttaggaggtgacaaa 
                   
                 
               
               
                   
               
               
                 
                   
                     gccctttcttcaattaatagagaaaaaatgtcttgttttttaagacggatgaaggatacaagtg 
                   
                 
               
               
                   
               
               
                 
                   
                     gaggtttcaggatgcatgatatgggagaaatggatgttcgtgcatgctacactgcaatttcggt 
                   
                 
               
               
                   
               
               
                     tgcaagcatcctaaatattatggatgatgaactcacccagggcctaggag   ctcgaatttccccg 
               
               
                   
               
               
                 atcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgat 
               
               
                   
               
               
                 tatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgtta 
               
               
                   
               
               
                 tttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaaca 
               
               
                   
               
               
                 aaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcggga 
               
               
                   
               
               
                 attcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcg 
               
               
                   
               
               
                 ccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgccct 
               
               
                   
               
               
                 tcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgcc 
               
               
                   
               
               
                 acgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtg 
               
               
                   
               
               
                 ctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgcc 
               
               
                   
               
               
                 ctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttc 
               
               
                   
               
               
                 caaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccga 
               
               
                   
               
               
                 tttcggaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctg 
               
               
                   
               
               
                 caactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaa 
               
               
                   
               
               
                 aaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gt   
               
               
                   
               
               
                 
                   ttacaccacaatatatcctgcca 
                 
               
               
                   
               
               
                 (Underline: RD29A promoter; Bold uppercase: antisense AtFTB; 
               
               
                 Lower case Bold: sense AtFTB) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 58 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatccatgccagtagtaacccgcttgattcgt ttgaagtgtgtagggctcagacttgaccggag   
               
               
                   
               
               
                 
                   tggactcaatcggcgaatctgtcacggaggacacggggaatcaacgcggcggagagtgatggaa 
                 
               
               
                   
               
               
                 
                   gagctttcaagcctaaccgtgagtcagcgcgagcaatttctggtggagaacgatgtgttcggga 
                 
               
               
                   
               
               
                 
                   tctataattacttcgacgccagcgacgtttctactcaaaaatacatgatggagattcagcgaga 
                 
               
               
                   
               
               
                 
                   taagcaattggattatctgatgaaaggcttaaggcagcttggtccgcagttttcttccttagat 
                 
               
               
                   
               
               
                 
                   gctaatcgaccttggctttgttactggattcttcattcaatagctttgcttggggagactgtgg 
                 
               
               
                   
               
               
                 
                   atgatgaattagaaagcaatgccattgacttccttggacgctgccagggctctgaaggtggata 
                 
               
               
                   
               
               
                 
                   cggtggtggtcctggccaacttccacatcttgcaactacttatgctgcagtgaatgcacttgtt 
                 
               
               
                   
               
               
                 
                   actttaggaggtgacaaagccctttcttcaattaatagagaaaaaatgtcttgttttttaagac 
                 
               
               
                   
               
               
                 
                   ggatgaaggatacaagtggaggtttcaggatgcatgatatgggagaaatggatgttcgtgcatg 
                 
               
               
                   
               
               
                 
                   ctacactgcaatttcggttgcaagcatcctaaatattatggatgatgaactcacccagggccta 
                 
               
               
                   
               
               
                 
                   ggagattacatcttgagttgccaaacttatgaaggtggcattggaggggaacctggctccgaag 
                 
               
               
                   
               
               
                 
                   ctcacggtgggtatacctactgtggtttggctgctatgattttaatcaatgaggtcgaccgttt 
                 
               
               
                   
               
               
                 
                   gaatttggattcattaatgaattgggctgtacatcgacaaggagtagaaatgggatttcaaggt 
                 
               
               
                   
               
               
                 
                   aggacgaacaaattggtcgatggttgctacacattttggcaggcagccccttgtgttctactac 
                 
               
               
                   
               
               
                 
                   aaagattatattcaaccaatgatcatgacgttcatggatcatcacatatatcagaagggacaaa 
                 
               
               
                   
               
               
                 
                   tgaagaacatcatgctcatgatgaagatgaccttgaagacagtgatgatgatgatgattctgat 
                 
               
               
                   
               
               
                 
                   gaggacaacgatgaagattcagtgaatggtcacagaatccatcatacatccacctacattaaca 
                 
               
               
                   
               
               
                 
                   ggagaatgcaactggtttttgatagcctcggcttgcagagatatgtactcttgtgctctaagat 
                 
               
               
                   
               
               
                 
                   ccctgacggtggattcagagacaagccgaggaaaccccgtgacttctaccacacatgttactgc 
                 
               
               
                   
               
               
                 
                   ctgagcggcttgtctgtggctcagcacgcttggttaaaagacgaggacactcctcctttgactc 
                 
               
               
                   
               
               
                 
                   gcgacattatgggtggctactcgaatctccttgaacctgttcaacttcttcacaacattgtcat 
                 
               
               
                   
               
               
                   ggatcagtataatgaagctatcgagttcttctttaaagcagcatga ctcgaatttccccgatcg 
               
               
                   
               
               
                 ttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatc 
               
               
                   
               
               
                 atataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttattta 
               
               
                   
               
               
                 tgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaat 
               
               
                   
               
               
                 atagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattc 
               
               
                   
               
               
                 actggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgcctt 
               
               
                   
               
               
                 gcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttccc 
               
               
                   
               
               
                 aacagttgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgt 
               
               
                   
               
               
                 tcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgcttt 
               
               
                   
               
               
                 acggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctga 
               
               
                   
               
               
                 tagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaa 
               
               
                   
               
               
                 ctggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttc 
               
               
                   
               
               
                 ggaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaac 
               
               
                   
               
               
                 tctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaac 
               
               
                   
               
               
                 caccccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttac   
               
               
                   
               
               
                 
                   accacaatatatcctgcca 
                 
               
               
                   
               
               
                 (Underlined: 35S promoter; Bold: Sense AtFTB) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 59 
                   
               
            
           
           
               
               
            
               
                 GAATTCAAATTTTTCGCCAGTTCTAAATATCCGGAAACCTCTTGGGATGCCATTGCCCATCTAT 
                   
               
               
                   
               
               
                 CTGTAATTTATTGACGAAATAGACGAAAAGGAAGGTGGCTCCTATAAAGCACATCATTGCGATA 
               
               
                   
               
               
                 ACAGAAAGGCCATTGTTGAAGATACCTCTGCTGACATTGGTCCCCAAGTGGAAGCACCACCCCA 
               
               
                   
               
               
                 TGAGGAGCACCGTGGAGTAAGAAGACGTTCGAGCCACGTCGAAAAAGCAAGTGTGTTGATGTAG 
               
               
                   
               
               
                 TATCTCCATTGACGTAAGGGATGACGCACAATCCAACTATCCATCGCAAGACCATTGCTCTATA 
               
               
                   
               
               
                 TAAGAAAGTTAATATCATTTCGAGTGGCCACGCTGAGCTC GTGGTGGAGAATCTGGGTGCTTTG   
               
               
                   
               
               
                 
                   ACCAACTATACTGGCACAATGAGAGTCCACTTAAACAGTAACATGTGTGATAATGATCTCTACG 
                 
               
               
                   
               
               
                 
                   TTTACCCGGTTTGTCTCTCAGTCCACCCTCTTGCTCCTGTGCACATAAGAGAATATATTGCTGT 
                 
               
               
                   
               
               
                 
                   AAAGCAATACTGTGAAAAAGTGGTTCTTGTGCTCTCCACTCATTAATAAATTTATAGGCAATAT 
                 
               
               
                   
               
               
                 
                   TTTTAAAATCAGATGAACTGGATTCACTGGTGCCTTCATGCTCACCACGGCATGTTGCATGACT 
                 
               
               
                   
               
               
                 
                   AGAGGTTCCATCCAAACTTTCTTTTGCTTCAGATACATAAGATACCGCAAAAATCTGTGATGTC 
                 
               
               
                   
               
               
                 
                   TCTTCCATCTGTTTGTTGATAATAGAAGATAATCTTTGCAATAGAGCAACAGCACCTCCCTGCC 
                 
               
               
                   
               
               
                 
                   AAAAGGAATAGCATCCATCCACCAGTTTATTTGTTCTCCCCTGGAATCCACATTCCTTACCTTG 
                 
               
               
                   
               
               
                 
                   TCGGAATACCACCCAGTCAACTAATCGAGGCAGATCCAAGTGATTAACCTCACCAATCAGAATC 
                 
               
               
                   
               
               
                 
                   ATTGTAGCTAATCCACAAAAGGTGTACCCACCATGAGCCTCAGAACCAGGCTCACCAGCAATGC 
                 
               
               
                   
               
               
                 
                   CACCCTCATATGTTTGACAGCTTATAATGTAGTCTCCAACATTCTGGATCAGCTCATCATCCAA 
                 
               
               
                   
               
               
                 
                   AATGTTCAAAACACTTGCAACAGAAATGGCAGTGTAGCAAGCTCGAACATCAATTTCACCTTCA 
                 
               
               
                   
               
               
                 
                   TCATGCATCCTGAATCCACCATTTGGTTGCTTCATCCGCCGCAGAAACCCATACAGTTTATCTC 
                 
               
               
                   
               
               
                 
                   TATTAATTGATGCCAGGGATTTCTCACCACCCAAAGTAATAAGTGAATTAACAGCAGCATAAGT 
                 
               
               
                   
               
               
                 
                   TGTGGCAATATGAGGCATCTGGCCTGGTCCCCCGGCATATCCACCATTCGGATCCTGGCAACGG 
                 
               
               
                   
               
               
                 
                   TTAAGAAAATCGATAGCGTTATCTTCGAGTTCATCATCGACGGATTCTCCCAACAAAGCAATGG 
                 
               
               
                   
               
               
                 
                   AGTGGAAGATCCAGTAGCAGAGCCAGGGTCGATTAGCGTCCAAAACGGAAAATGCGGAACTGAG 
                 
               
               
                   
               
               
                 
                   ATGGCGAAGGCCTTTGGAGACATACTGCATGTGATTATCGCGTTGAAGCTCCAACATGAGGGTT 
                 
               
               
                   
               
               
                   TGGGCGTTGCGAGGAATGGTGGC gagct c gaatttccccgatcgttcaaacatttggcaataaa 
               
               
                   
               
               
                 gtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaatta 
               
               
                   
               
               
                 cgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgatt 
               
               
                   
               
               
                 agagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggata 
               
               
                   
               
               
                 aattatcgcgcgcggtgtcatctatgttactagatcgggaattc 
               
               
                   
               
               
                 (Upper Case: MuA Promoter; Underlined: Antisense GmFTB; Lowercase: 
               
               
                 NOS terminater) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 60 
                   
               
            
           
           
               
               
            
               
                 GGAGCCATAGATGCAATTCAATCAAACTGAAATTTCTGCAAGAATCTCAAACACGGAGATCTCA 
                   
               
               
                   
               
               
                 AAGTTTGAAAGAAAATTTATTTCTTCGACTCAAAACAAACTTACGAAATTTAGGTAGAACTTAT 
               
               
                   
               
               
                 ATACATTATATTGTAATTTTTTGTAACAAAATGTTTTTATTATTATTATAGAATTTTACTGGTT 
               
               
                   
               
               
                 AAATTAAAAATGAATAGAAAAGGTGAATTAAGAGGAGAGAGGAGGTAAACATTTTCTTCTATTT 
               
               
                   
               
               
                 TTTCATATTTTCAGGATAAATTATTGTAAAAGTTTACAAGATTTCCATTTGACTAGTGTAAATG 
               
               
                   
               
               
                 AGGAATATTCTCTAGTAAGATCATTATTTCATCTACTTCTTTTATCTTCTACCAGTAGAGGAAT 
               
               
                   
               
               
                 AAACAATATTTAGCTCCTTTGTAAATACAAATTAATTTTCCTTCTTGACATCATTCAATTTTAA 
               
               
                   
               
               
                 TTTTACGTATAAAATAAAAGATCATACCTATTAGAACGATTAAGGAGAAATACAATTCGAATGA 
               
               
                   
               
               
                 GAAGGATGTGCCGTTTGTTATAATAAACAGCCACACGACGTAAACGTAAAATGACCACATGATG 
               
               
                   
               
               
                 GGCCAATAGACATGGACCGACTACTAATAATAGTAAGTTACATTTTAGGATGGAATAAATATCA 
               
               
                   
               
               
                 TACCGACATCAGTTTTGAAAGAAAAGGGAAAAAAAGAAAAAATAAATAAAAGATATACTACCGA 
               
               
                   
               
               
                 CATGAGTTCCAAAAAGCAAAAAAAAAGATCAAGCCGACACAGACACGCGTAGAGAGCAAAATGA 
               
               
                   
               
               
                 CTTTGACGTCACACCACGAAAACAGACGCTTCATACGTGTCCCTTTATCTCTCTCAGTCTCTCT 
               
               
                   
               
               
                 ATAAACTTAGTGAGACCCTCCTCTGTTTTACTCACAAATATGCAAACTAGAAAACAATCATCAG 
               
               
                   
               
               
                 GAATAAAGGGTTTGATTACTTCTATTGGAAAG GTGGTGGAGAATCTGGGTGCTTTGACCAACTA   
               
               
                   
               
               
                 
                   TACTGGCACAATGAGAGTCCACTTAAACAGTAACATGTGTGATAATGATCTCTACGTTTACCCG 
                 
               
               
                   
               
               
                 
                   GTTTGTCTCTCAGTCCACCCTCTTGCTCCTGTGCACATAAGAGAATATATTGCTGTAAAGCAAT 
                 
               
               
                   
               
               
                 
                   ACTGTGAAAAAGTGGTTCTTGTGCTCTCCACTCATTAATAAATTTATAGGCAATATTTTTAAAA 
                 
               
               
                   
               
               
                 
                   TCAGATGAACTGGATTCACTGGTGCCTTCATGCTCACCACGGCATGTTGCATGACTAGAGGTTC 
                 
               
               
                   
               
               
                 
                   CATCCAAACTTTCTTTTGCTTCAGATACATAAGATACCGCAAAAATCTGTGATGTCTCTTCCAT 
                 
               
               
                   
               
               
                 
                   CTGTTTGTTGATAATAGAAGATAATCTTTGCAATAGAGCAACAGCACCTCCCTGCCAAAAGGAA 
                 
               
               
                   
               
               
                 
                   TAGCATCCATCCACCAGTTTATTTGTTCTCCCCTGGAATCCACATTCCTTACCTTGTCGGAATA 
                 
               
               
                   
               
               
                 
                   CCACCCAGTCAACTAATCGAGGCAGATCCAAGTGATTAACCTCACCAATCAGAATCATTGTAGC 
                 
               
               
                   
               
               
                 
                   TAATCCACAAAAGGTGTACCCACCATGAGCCTCAGAACCAGGCTCACCAGCAATGCCACCCTCA 
                 
               
               
                   
               
               
                 
                   TATGTTTGACAGCTTATAATGTAGTCTCCAACATTCTGGATCAGCTCATCATCCAAAATGTTCA 
                 
               
               
                   
               
               
                 
                   AAACACTTGCAACAGAAATGGCAGTGTAGCAAGCTCGAACATCAATTTCACCTTCATCATGCAT 
                 
               
               
                   
               
               
                 
                   CCTGAATCCACCATTTGGTTGCTTCATCCGCCGCAGAAACCCATACAGTTTATCTCTATTAATT 
                 
               
               
                   
               
               
                 
                   GATGCCAGGGATTTCTCACCACCCAAAGTAATAAGTGAATTAACAGCAGCATAAGTTGTGGCAA 
                 
               
               
                   
               
               
                 
                   TATGAGGCATCTGGCCTGGTCCCCCGGCATATCCACCATTCGGATCCTGGCAACGGTTAAGAAA 
                 
               
               
                   
               
               
                 
                   ATCGATAGCGTTATCTTCGAGTTCATCATCGACGGATTCTCCCAACAAAGCAATGGAGTGGAAG 
                 
               
               
                   
               
               
                 
                   ATCCAGTAGCAGAGCCAGGGTCGATTAGCGTCCAAAACGGAAAATGCGGAACTGAGATGGCGAA 
                 
               
               
                   
               
               
                 
                   GGCCTTTGGAGACATACTGCATGTGATTATCGCGTTGAAGCTCCAACATGAGGGTTTGGGCGTT 
                 
               
               
                   
               
               
                   GCGAGGAATGGTGGC gagct c gaatttccccgatcgttcaaacatttggcaataaagtttctta 
               
               
                   
               
               
                 agattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagc 
               
               
                   
               
               
                 atgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtccc 
               
               
                   
               
               
                 gcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcg 
               
               
                   
               
               
                 cgcgcggtgtcatctatgttactagatcgggaattc 
               
               
                   
               
               
                 (Upper Case: RD29A Promoter; Underlined: Antisense GmFTB; Lower 
               
               
                 case: NOS) terminater 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 61 
                   
               
            
           
           
               
               
            
               
                 GAATTCAAATTTTTCGCCAGTTCTAAATATCCGGAAACCTCTTGGGATGCCATTGCCCATCTAT 
                   
               
               
                   
               
               
                 CTGTAATTTATTGACGAAATAGACGAAAAGGAAGGTGGCTCCTATAAAGCACATCATTGCGATA 
               
               
                   
               
               
                 ACAGAAAGGCCATTGTTGAAGATACCTCTGCTGACATTGGTCCCCAAGTGGAAGCACCACCCCA 
               
               
                   
               
               
                 TGAGGAGCACCGTGGAGTAAGAAGACGTTCGAGCCACGTCGAAAAAGCAAGTGTGTTGATGTAG 
               
               
                   
               
               
                 TATCTCCATTGACGTAAGGGATGACGCACAATCCAACTATCCATCGCAAGACCATTGCTCTATA 
               
               
                   
               
               
                 TAAGAAAGTTAATATCATTTCGAGTGGCCACGCTGAGCTC GTGGTGGAGAATCTGGGTGCTTTG   
               
               
                   
               
               
                 
                   ACCAACTATACTGGCACAATGAGAGTCCACTTAAACAGTAACATGTGTGATAATGATCTCTACG 
                 
               
               
                   
               
               
                 
                   TTTACCCGGTTTGTCTCTCAGTCCACCCTCTTGCTCCTGTGCACATAAGAGAATATATTGCTGT 
                 
               
               
                   
               
               
                 
                   AAAGCAATACTGTGAAAAAGTGGTTCTTGTGCTCTCCACTCATTAATAAATTTATAGGCAATAT 
                 
               
               
                   
               
               
                 
                   TTTTAAAATCAGATGAACTGGATTCACTGGTGCCTTCATGCTCACCACGGCATGTTGCATGACT 
                 
               
               
                   
               
               
                 
                   AGAGGTTCCATCCAAACTTTCTTTTGCTTCAGATACATAAGATACCGCAAAAATCTGTGATGTC 
                 
               
               
                   
               
               
                 
                   TCTTCCATCTGTTTGTTGATAATAGAAGATAATCTTTGCAATAGAGCAACAGCACCTCCCTGCC 
                 
               
               
                   
               
               
                 
                   AAAAGGAATAGCATCCATCCACCAGTTTATTTGTTCTCCCCTGGAATCCACATTCCTTACCTTG 
                 
               
               
                   
               
               
                 
                   TCGGAATACCACCCAGTCAACTAATCGAGGCAGATCCAAGTGATTAACCTCACCAATCAGAATC 
                 
               
               
                   
               
               
                 
                   ATTGTAGCTAATCCACAAAAGGTGTACCCACCATGAGCCTCAGAACCAGGCTCACCAGCAATGC 
                 
               
               
                   
               
               
                 
                   CACCCTCATATGTTTGACAGCTTATAATGTAGTCTCCAACATTCTGGATCAGCTCATCATCCAA 
                 
               
               
                   
               
               
                 
                   AATGTTCAAAACACTTGCAACAGAAATGGCAGTGTAGCAAGCTCGAACATCAATTTCACCTTCA 
                 
               
               
                   
               
               
                 
                   TCATGCATCCTGAATCCACCATTTGGTTGCTTCATCCGCCGCAGAAACCCATACAGTTTATCTC 
                 
               
               
                   
               
               
                 
                   TATTAATTGATGCCAGGGATTTCTCACCACCCAAAGTAATAAGTGAATTAACAGCAGCATAAGT 
                 
               
               
                   
               
               
                 
                   TGTGGCAATATGAGGCATCTGGCCTGGTCCCCCGGCATATCCACCATTCGGATCCTGGCAACGG 
                 
               
               
                   
               
               
                 
                   TTAAGAAAATCGATAGCGTTATCTTCGAGTTCATCATCGACGGATTCTCCCAACAAAGCAATGG 
                 
               
               
                   
               
               
                 
                   AGTGGAAGATCCAGTAGCAGAGCCAGGGTCGATTAGCGTCCAAAACGGAAAATGCGGAACTGAG 
                 
               
               
                   
               
               
                 
                   ATGGCGAAGGCCTTTGGAGACATACTGCATGTGATTATCGCGTTGAAGCTCCAACATGAGGGTT 
                 
               
               
                   
               
               
                 
                   TGGGCGTTGCGAGGAATGGTGGC 
                   GGTGAGGTTAATCACTTGGATCTGCCTCGATTAGTTGACTG 
                 
               
               
                   
               
               
                 
                   GGTGGTATTCCGACAAGGTAAGGAATGTGGATTCCAGGGGAGAACAAATAAACTGGTGGATGGA 
                 
               
               
                   
               
               
                 
                   TGCTATTCCTTTTGGCAGGGAGGTGCTGTTGCTCTATTGCAAAGATTATCTTCTATTATCAACA 
                 
               
               
                   
               
               
                 
                   AACAGATGGAAGAGACATCACAGATTTTTGCGGTATCTTATGTATCTGAAGCAAAAGAAAGTTT 
                 
               
               
                   
               
               
                 
                   GGATGGAACCTCTAGTCATGCAACATGCCGTGGTGAGCATGAAGGCACCAGTGAATCCAGTTCA 
                 
               
               
                   
               
               
                 
                   TCTGATTTTAAAAATATTGCCTATAAATTTATTAATGAGTGGAGAGCACAAGAACCACTTTTTC 
                 
               
               
                   
               
               
                 
                   ACAGTATTGCTTTACAGCAATATATTCTCTTATGTGCACAGGAGCAAGAGGGTGGACTGAGAGA 
                 
               
               
                   
               
               
                 
                   CAAACCGGGTAAACGTAGAGATCATTATCACACATGTTACTGTTTAAGTGGACTCTCATTGTGC 
                 
               
               
                   
               
               
                   CAGTATAGTTGGTCAAAGCACCCAGATTCTCCACCACgagctc gaatttccccgatcgttcaaa 
               
               
                   
               
               
                 catttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataa 
               
               
                   
               
               
                 tttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagat 
               
               
                   
               
               
                 gggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcg 
               
               
                   
               
               
                 cgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattc 
               
               
                   
               
               
                 (Upper Case: MuA Promoter; Underlined: Antisense GmFTB; Bold: 
               
               
                 Sense GmFTB; Lower case: NOS terminater) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 62 
                   
               
            
           
           
               
               
            
               
                 GGAGCCATAGATGCAATTCAATCAAACTGAAATTTCTGCAAGAATCTCAAACACGGAGATCTCA 
                   
               
               
                   
               
               
                 AAGTTTGAAAGAAAATTTATTTCTTCGACTCAAAACAAACTTACGAAATTTAGGTAGAACTTAT 
               
               
                   
               
               
                 ATACATTATATTGTAATTTTTTGTAACAAAATGTTTTTATTATTATTATAGAATTTTACTGGTT 
               
               
                   
               
               
                 AAATTAAAAATGAATAGAAAAGGTGAATTAAGAGGAGAGAGGAGGTAAACATTTTCTTCTATTT 
               
               
                   
               
               
                 TTTCATATTTTCAGGATAAATTATTGTAAAAGTTTACAAGATTTCCATTTGACTAGTGTAAATG 
               
               
                   
               
               
                 AGGAATATTCTCTAGTAAGATCATTATTTCATCTACTTCTTTTATCTTCTACCAGTAGAGGAAT 
               
               
                   
               
               
                 AAACAATATTTAGCTCCTTTGTAAATACAAATTAATTTTCCTTCTTGACATCATTCAATTTTAA 
               
               
                   
               
               
                 TTTTACGTATAAAATAAAAGATCATACCTATTAGAACGATTAAGGAGAAATACAATTCGAATGA 
               
               
                   
               
               
                 GAAGGATGTGCCGTTTGTTATAATAAACAGCCACACGACGTAAACGTAAAATGACCACATGATG 
               
               
                   
               
               
                 GGCCAATAGACATGGACCGACTACTAATAATAGTAAGTTACATTTTAGGATGGAATAAATATCA 
               
               
                   
               
               
                 TACCGACATCAGTTTTGAAAGAAAAGGGAAAAAAAGAAAAAATAAATAAAAGATATACTACCGA 
               
               
                   
               
               
                 CATGAGTTCCAAAAAGCAAAAAAAAAGATCAAGCCGACACAGACACGCGTAGAGAGCAAAATGA 
               
               
                   
               
               
                 CTTTGACGTCACACCACGAAAACAGACGCTTCATACGTGTCCCTTTATCTCTCTCAGTCTCTCT 
               
               
                   
               
               
                 ATAAACTTAGTGAGACCCTCCTCTGTTTTACTCACAAATATGCAAACTAGAAAACAATCATCAG 
               
               
                   
               
               
                 GAATAAAGGGTTTGATTACTTCTATTGGAAAG GTGGTGGAGAATCTGGGTGCTTTGACCAACTA   
               
               
                   
               
               
                 
                   TACTGGCACAATGAGAGTCCACTTAAACAGTAACATGTGTGATAATGATCTCTACGTTTACCCG 
                 
               
               
                   
               
               
                 
                   GTTTGTCTCTCAGTCCACCCTCTTGCTCCTGTGCACATAAGAGAATATATTGCTGTAAAGCAAT 
                 
               
               
                   
               
               
                 
                   ACTGTGAAAAAGTGGTTCTTGTGCTCTCCACTCATTAATAAATTTATAGGCAATATTTTTAAAA 
                 
               
               
                   
               
               
                 
                   TCAGATGAACTGGATTCACTGGTGCCTTCATGCTCACCACGGCATGTTGCATGACTAGAGGTTC 
                 
               
               
                   
               
               
                 
                   CATCCAAACTTTCTTTTGCTTCAGATACATAAGATACCGCAAAAATCTGTGATGTCTCTTCCAT 
                 
               
               
                   
               
               
                 
                   CTGTTTGTTGATAATAGAAGATAATCTTTGCAATAGAGCAACAGCACCTCCCTGCCAAAAGGAA 
                 
               
               
                   
               
               
                 
                   TAGCATCCATCCACCAGTTTATTTGTTCTCCCCTGGAATCCACATTCCTTACCTTGTCGGAATA 
                 
               
               
                   
               
               
                 
                   CCACCCAGTCAACTAATCGAGGCAGATCCAAGTGATTAACCTCACCAATCAGAATCATTGTAGC 
                 
               
               
                   
               
               
                 
                   TAATCCACAAAAGGTGTACCCACCATGAGCCTCAGAACCAGGCTCACCAGCAATGCCACCCTCA 
                 
               
               
                   
               
               
                 
                   TATGTTTGACAGCTTATAATGTAGTCTCCAACATTCTGGATCAGCTCATCATCCAAAATGTTCA 
                 
               
               
                   
               
               
                 
                   AAACACTTGCAACAGAAATGGCAGTGTAGCAAGCTCGAACATCAATTTCACCTTCATCATGCAT 
                 
               
               
                   
               
               
                 
                   CCTGAATCCACCATTTGGTTGCTTCATCCGCCGCAGAAACCCATACAGTTTATCTCTATTAATT 
                 
               
               
                   
               
               
                 
                   GATGCCAGGGATTTCTCACCACCCAAAGTAATAAGTGAATTAACAGCAGCATAAGTTGTGGCAA 
                 
               
               
                   
               
               
                 
                   TATGAGGCATCTGGCCTGGTCCCCCGGCATATCCACCATTCGGATCCTGGCAACGGTTAAGAAA 
                 
               
               
                   
               
               
                 
                   ATCGATAGCGTTATCTTCGAGTTCATCATCGACGGATTCTCCCAACAAAGCAATGGAGTGGAAG 
                 
               
               
                   
               
               
                 
                   ATCCAGTAGCAGAGCCAGGGTCGATTAGCGTCCAAAACGGAAAATGCGGAACTGAGATGGCGAA 
                 
               
               
                   
               
               
                 
                   GGCCTTTGGAGACATACTGCATGTGATTATCGCGTTGAAGCTCCAACATGAGGGTTTGGGCGTT 
                 
               
               
                   
               
               
                 
                   GCGAGGAATGGTGGC 
                   GGTGAGGTTAATCACTTGGATCTGCCTCGATTAGTTGACTGGGTGGTAT 
                 
               
               
                   
               
               
                 
                   TCCGACAAGGTAAGGAATGTGGATTCCAGGGGAGAACAAATAAACTGGTGGATGGATGCTATTC 
                 
               
               
                   
               
               
                 
                   CTTTTGGCAGGGAGGTGCTGTTGCTCTATTGCAAAGATTATCTTCTATTATCAACAAACAGATG 
                 
               
               
                   
               
               
                 
                   GAAGAGACATCACAGATTTTTGCGGTATCTTATGTATCTGAAGCAAAAGAAAGTTTGGATGGAA 
                 
               
               
                   
               
               
                 
                   CCTCTAGTCATGCAACATGCCGTGGTGAGCATGAAGGCACCAGTGAATCCAGTTCATCTGATTT 
                 
               
               
                   
               
               
                 
                   TAAAAATATTGCCTATAAATTTATTAATGAGTGGAGAGCACAAGAACCACTTTTTCACAGTATT 
                 
               
               
                   
               
               
                 
                   GCTTTACAGCAATATATTCTCTTATGTGCACAGGAGCAAGAGGGTGGACTGAGAGACAAACCGG 
                 
               
               
                   
               
               
                 
                   GTAAACGTAGAGATCATTATCACACATGTTACTGTTTAAGTGGACTCTCATTGTGCCAGTATAG 
                 
               
               
                   
               
               
                   TTGGTCAAAGCACCCAGATTCTCCACCACgagctc gaatttccccgatcgttcaaacatttggc 
               
               
                   
               
               
                 aataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgtt 
               
               
                   
               
               
                 gaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggttttt 
               
               
                   
               
               
                 atgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaact 
               
               
                   
               
               
                 aggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattc 
               
               
                   
               
               
                 (Upper Case: RD29A Promoter; Underlined: Antisense GmFTB; Bold: 
               
               
                 Sense GmFTB; Lower case: NOS terminater) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 63 
                   
               
            
           
           
               
               
            
               
                 GAATTCAAATTTTTCGCCAGTTCTAAATATCCGGAAACCTCTTGGGATGCCATTGCCCATCTAT 
                   
               
               
                   
               
               
                 CTGTAATTTATTGACGAAATAGACGAAAAGGAAGGTGGCTCCTATAAAGCACATCATTGCGATA 
               
               
                   
               
               
                 ACAGAAAGGCCATTGTTGAAGATACCTCTGCTGACATTGGTCCCCAAGTGGAAGCACCACCCCA 
               
               
                   
               
               
                 TGAGGAGCACCGTGGAGTAAGAAGACGTTCGAGCCACGTCGAAAAAGCAAGTGTGTTGATGTAG 
               
               
                   
               
               
                 TATCTCCATTGACGTAAGGGATGACGCACAATCCAACTATCCATCGCAAGACCATTGCTCTATA 
               
               
                   
               
               
                 TAAGAAAGTTAATATCATTTCGAGTGGCCACGCTGAGCTC GGATGGATTGGCTCCAGCAAATTA   
               
               
                   
               
               
                 
                   GAGTACGGTCCAAGCACATGCTGAGGTAATGGGCACGAACCAGTATCAGTCATGGCACTGTACT 
                 
               
               
                   
               
               
                 
                   GGCTAACTGCGAGGCCACTGAGGCAGTAGCATGAATGATAGTGATCTCTGTTCTTTCCAGGCTT 
                 
               
               
                   
               
               
                 
                   ATCCCTCAAGCCTCCCTCTAGTACCTGAGAACAAAGTAGGATGTATTGTTGCAGGGCAATGTTA 
                 
               
               
                   
               
               
                 
                   TGGAAGAGTGGGCCAATTTGGTTGCTCTGTTGTATAAAATCAAATCCAAACTTCGCATAGTCCA 
                 
               
               
                   
               
               
                 
                   CAGCAGAGGAAGACTTATTCGCGGTGCACCCATATGAACTGGTGCTGCAGGCATCCTCTCCTGA 
                 
               
               
                   
               
               
                 
                   TGGCCTTTTGCAGGAATACGAGGACCTCAATTGCTTATCAACAATCGTAATTAACTTTTGTGTG 
                 
               
               
                   
               
               
                 
                   AAAGCAATGGCAGCTCCCTGCCAAAAGGAGTAGCAACCATCAACCAATTTATTAGTTCGTCCTT 
                 
               
               
                   
               
               
                 
                   GAAATCCGCATTCCACTCCTTGACGAAAAGCCACCCAGCCAATCAAACTAGGCAAGTCAACTTT 
                 
               
               
                   
               
               
                 
                   CTCTGCCTCATTAAGCAGGATCAAAGCAGCCAATCCACAGAATGTATACCCACCATGTGCTTCA 
                 
               
               
                   
               
               
                 
                   GCATAAGGCTCCCCAGCAATACCACCTTCATAAGTTTGACATCTTGCTATGTAGTCGCCTACAC 
                 
               
               
                   
               
               
                 
                   CTTTTGCCAGTTTAAAATCAAGAATATTCACAAGGCTGGCAACCGATATAGCGGTGTAGGAAGC 
                 
               
               
                   
               
               
                 
                   ACGGACATCAATTTCGCCACCATCATGCATTCTGAAAGCACCTGATACATCTTTCATCTGCAGC 
                 
               
               
                   
               
               
                 
                   ATAAAATTGTACAGGTTGCCCCTATTGATTGATGACAATGCTCTTTCGCTCCCTATTGTCACAA 
                 
               
               
                   
               
               
                 
                   GTGTATTTACAGCAGCATAAGTCGTAGCTAGGTGAGGCAACTGTCCAGGTCCACCACTATATCC 
                 
               
               
                   
               
               
                 
                   ACCATCTTTATCCTGACATCGAGCTAAGAAGTCTATGATATCATTCTCAAGATCATCATCAAGT 
                 
               
               
                   
               
               
                 
                   GCTTCATCCAGCAAAGCAAGTGGATGAACCATCCAGTAGCATAGCCAAGGGCGATTGGCATCTA 
                 
               
               
                   
               
               
                 
                   GAACATGAAAGGCTGGTCCCATATGCCTCAGCCCAGGCGTCAGATACTCGATATGCTGATCACG 
                 
               
               
                   
               
               
                 
                   CCACAGCTCTAGCATGATGGATTTCGTGTTGGGCGCGGCCCCGAAGAGGGAGCGGTAGATGTCG 
                 
               
               
                   
               
               
                 
                   CCAACCCTGGCCTCCACCTTCATCTGCTCCACCTGCGTCACCGTGAGCCTCGGTAGGTCGGGAT 
                 
               
               
                   
               
               
                   CCGCC gagct c gaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatc 
               
               
                   
               
               
                 ctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataat 
               
               
                   
               
               
                 taacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatac 
               
               
                   
               
               
                 atttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgt 
               
               
                   
               
               
                 catctatgttactagatcgggaattc 
               
               
                   
               
               
                 (Upper Case: MuA Promoter; Underlined: Antisense  Zea  maize- 
               
               
                 FTB; Lowercase: NOS terminater) 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO: 64 
                   
               
            
           
           
               
               
            
               
                 GAATTCAAATTTTTCGCCAGTTCTAAATATCCGGAAACCTCTTGGGATGCCATTGCCCATCTAT 
                   
               
               
                   
               
               
                 CTGTAATTTATTGACGAAATAGACGAAAAGGAAGGTGGCTCCTATAAAGCACATCATTGCGATA 
               
               
                   
               
               
                 ACAGAAAGGCCATTGTTGAAGATACCTCTGCTGACATTGGTCCCCAAGTGGAAGCACCACCCCA 
               
               
                   
               
               
                 TGAGGAGCACCGTGGAGTAAGAAGACGTTCGAGCCACGTCGAAAAAGCAAGTGTGTTGATGTAG 
               
               
                   
               
               
                 TATCTCCATTGACGTAAGGGATGACGCACAATCCAACTATCCATCGCAAGACCATTGCTCTATA 
               
               
                   
               
               
                 TAAGAAAGTTAATATCATTTCGAGTGGCCACGCTGAGCTC GGATGGATTGGCTCCAGCAAATTA   
               
               
                   
               
               
                 
                   GAGTACGGTCCAAGCACATGCTGAGGTAATGGGCACGAACCAGTATCAGTCATGGCACTGTACT 
                 
               
               
                   
               
               
                 
                   GGCTAACTGCGAGGCCACTGAGGCAGTAGCATGAATGATAGTGATCTCTGTTCTTTCCAGGCTT 
                 
               
               
                   
               
               
                 
                   ATCCCTCAAGCCTCCCTCTAGTACCTGAGAACAAAGTAGGATGTATTGTTGCAGGGCAATGTTA 
                 
               
               
                   
               
               
                 
                   TGGAAGAGTGGGCCAATTTGGTTGCTCTGTTGTATAAAATCAAATCCAAACTTCGCATAGTCCA 
                 
               
               
                   
               
               
                 
                   CAGCAGAGGAAGACTTATTCGCGGTGCACCCATATGAACTGGTGCTGCAGGCATCCTCTCCTGA 
                 
               
               
                   
               
               
                 
                   TGGCCTTTTGCAGGAATACGAGGACCTCAATTGCTTATCAACAATCGTAATTAACTTTTGTGTG 
                 
               
               
                   
               
               
                 
                   AAAGCAATGGCAGCTCCCTGCCAAAAGGAGTAGCAACCATCAACCAATTTATTAGTTCGTCCTT 
                 
               
               
                   
               
               
                 
                   GAAATCCGCATTCCACTCCTTGACGAAAAGCCACCCAGCCAATCAAACTAGGCAAGTCAACTTT 
                 
               
               
                   
               
               
                 
                   CTCTGCCTCATTAAGCAGGATCAAAGCAGCCAATCCACAGAATGTATACCCACCATGTGCTTCA 
                 
               
               
                   
               
               
                 
                   GCATAAGGCTCCCCAGCAATACCACCTTCATAAGTTTGACATCTTGCTATGTAGTCGCCTACAC 
                 
               
               
                   
               
               
                 
                   CTTTTGCCAGTTTAAAATCAAGAATATTCACAAGGCTGGCAACCGATATAGCGGTGTAGGAAGC 
                 
               
               
                   
               
               
                 
                   ACGGACATCAATTTCGCCACCATCATGCATTCTGAAAGCACCTGATACATCTTTCATCTGCAGC 
                 
               
               
                   
               
               
                 
                   ATAAAATTGTACAGGTTGCCCCTATTGATTGATGACAATGCTCTTTCGCTCCCTATTGTCACAA 
                 
               
               
                   
               
               
                 
                   GTGTATTTACAGCAGCATAAGTCGTAGCTAGGTGAGGCAACTGTCCAGGTCCACCACTATATCC 
                 
               
               
                   
               
               
                 
                   ACCATCTTTATCCTGACATCGAGCTAAGAAGTCTATGATATCATTCTCAAGATCATCATCAAGT 
                 
               
               
                   
               
               
                 
                   GCTTCATCCAGCAAAGCAAGTGGATGAACCATCCAGTAGCATAGCCAAGGGCGATTGGCATCTA 
                 
               
               
                   
               
               
                 
                   GAACATGAAAGGCTGGTCCCATATGCCTCAGCCCAGGCGTCAGATACTCGATATGCTGATCACG 
                 
               
               
                   
               
               
                 
                   CCACAGCTCTAGCATGATGGATTTCGTGTTGGGCGCGGCCCCGAAGAGGGAGCGGTAGATGTCG 
                 
               
               
                   
               
               
                 
                   CCAACCCTGGCCTCCACCTTCATCTGCTCCACCTGCGTCACCGTGAGCCTCGGTAGGTCGGGAT 
                 
               
               
                   
               
               
                 
                   CCGCCggatcc 
                   GCTGGGGAGCCTTATGCTGAAGCACATGGTGGGTATACATTCTGTGGATTGGC 
                 
               
               
                   
               
               
                 
                   TGCTTTGATCCTGCTTAATGAGGCAGAGAAAGTTGACTTGCCTAGTTTGATTGGCTGGGTGGCT 
                 
               
               
                   
               
               
                 
                   TTTCGTCAAGGAGTGGAATGCGGATTTCAAGGACGAACTAATAAATTGGTTGATGGTTGCTACT 
                 
               
               
                   
               
               
                 
                   CCTTTTGGCAGGGAGCTGCCATTGCTTTCACACAAAAGTTAATTACGATTGTTGATAAGCAATT 
                 
               
               
                   
               
               
                 
                   GAGGTCCTCGTATTCCTGCAAAAGGCCATCAGGAGAGGATGCCTGCAGCACCAGTTCATATGGG 
                 
               
               
                   
               
               
                 
                   TGCACCGCGAATAAGTCTTCCTCTGCTGTGGACTATGCGAAGTTTGGATTTGATTTTATACAAC 
                 
               
               
                   
               
               
                 
                   AGAGCAACCAAATTGGCCCACTCTTCCATAACATTGCCCTGCAACAATACATCCTACTTTGTTC 
                 
               
               
                   
               
               
                 
                   TCAGGTACTAGAGGGAGGCTTGAGGGATAAGCCTGGAAAGAACAGAGATCACTATCATTCATGC 
                 
               
               
                   
               
               
                 
                   TACTGCCTCAGTGGCCTCGCAGTTAGCCAGTACAGTGCCATGACTGATACTGGTTCGTGCCCAT 
                 
               
               
                   
               
               
                   TACCTCAGCATGTGCTTGGACCGTACTCTAATTTGCTGGAGCCAATCCATCC aagcttgaattt 
               
               
                   
               
               
                 ccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcg 
               
               
                   
               
               
                 atgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatga 
               
               
                   
               
               
                 cgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgataga 
               
               
                   
               
               
                 aaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagat 
               
               
                   
               
               
                 cggaagctt 
               
               
                   
               
               
                 (Upper Case: MuA Promoter; Underlined: Antisense  Zea  maize- 
               
               
                 FTB; Bold: Sense  Zea  maize-FTB; Lower case: NOS terminater) 
               
            
           
         
       
     
     Example 15 
     PCR Analysis of Putative Transgenic Plants 
     To verify that the putative transgenic plants carried the gene of interest PCR analysis was performed. Genomic DNA was isolated and PCR run according to standard protocols and conditions which are known to one of skill in the art. A typical reaction was performed in a volume of 25 μl and primer pairs used were dependent on the gene and promoter combination of the particular construct (Table 12). 
     Putative transgenic  Brassica napus  plants were screened using the primer combinations detailed in the table below. A representative gel showing PCR analysis results is shown in  FIG. 24  which represents transgenic plants carrying the pRD29A-anti-FTA construct. Transformants were confirmed in an analogous manner for each species and construct transformation done. 
     
       
         
           
               
               
               
             
               
                 TABLE 12 
               
               
                   
               
               
                 Construct 
                   
                   
               
               
                 Name 
                 Primer Name 
                 Primer Sequence (5′-3′) 
               
               
                   
               
             
            
               
                 35S-antiFTA 
                 SEQ ID NO: 16 
                 GCCGACAGTGGTCCCAAAGATGG 
               
               
                   
               
               
                   
                 SEQ ID NO: 17 
                 AAAGGATCCTCAAATTGCTGCCA 
               
               
                   
                   
                 CTGTAAT 
               
               
                   
               
               
                 rd29A-antiFTA 
                 SEQ ID NO: 18 
                 AAACCCGGGATGAATTTCGACG 
               
               
                   
                   
                 AGAACGTG 
               
               
                   
               
               
                   
                 SEQ ID NO: 19 
                 GCAAGACCGGCAACAGGA 
               
               
                   
               
               
                 rd29B-antiFTA 
                 SEQ ID NO: 20 
                 TTTAAGCTTGACAGAAACAGT 
               
               
                   
                   
                 CAGCGAGAC 
               
               
                   
               
               
                   
                 SEQ ID NO: 17 
                 AAACCCGGGATGAATTTCGACG 
               
               
                   
                   
                 AGAACGTG 
               
               
                   
               
               
                 35S-DA-FTA 
                 SEQ ID NO: 21 
                 GCTCTTCCTCCATGCCCA 
               
               
                   
               
               
                   
                 SEQ ID NO: 19 
                 GCAAGACCGGCAACAGGA 
               
               
                   
               
               
                 rd29A-DA-FTA 
                 SEQ ID NO: 22 
                 TTTAAGCTTGGAGCCATAGA 
               
               
                   
                   
                 TGCAATTCAA 
               
               
                   
               
               
                   
                 SEQ ID NO: 23 
                 CGGGCATTAGGAGGATGGGAA 
               
               
                   
               
               
                 35S-HP-FTB 
                 SEQ ID NO: 16 
                 GCCGACAGTGGTCCCAAAGATGG 
               
               
                   
               
               
                   
                 SEQ ID NO: 24 
                 GTCCGGAATTCCCGGGTC 
               
               
                   
               
               
                 rd29A-HP-FTB 
                 SEQ ID NO: 22 
                 TTTAAGCTTGGAGCCATAG 
               
               
                   
                   
                 ATGCAATTCAA 
               
               
                   
               
               
                   
                 SEQ ID NO: 24 
                 GTCCGGAATTCCCGGGTC 
               
               
                   
               
            
           
         
       
     
     Example 16 
     Southern Analysis 
     Genomic Southern analysis of anti-FTA transgenic  Arabidopsis thaliana . The numbers indicate the line numbers. Five micrograms of genomic DNA of T1 plants was digested with HindIII (a unique site in the T-DNA plasmid) and separated in a 0.8% agarose gel. The NPTII coding region was used as the probe for radio-labeling.  FIG. 11  shows a typical result from Southern analysis indicating the presence of the transgene. 
     Example 17 
     Northern Blots of Antisense FTA Lines 
     RNA was isolated from developing leaf tissue of five 35S-anti-FTA  Arabidopsis thaliana  lines (T3 plants). The blot was first probed with P 32  labeled, single-stranded sense transcript of FTA ( FIG. 3  panel A) which detects antisense transcript, then stripped and re-probed with the single-stranded anti-sense transcript of FTA ( FIG. 12  panel B) that detects the sense transcript.  FIG. 3  panel C shows the ethidium bromide stained gel for the blot. Approximately 5 μg of total RNA was loaded into each lane.  FIG. 3  indicates the accumulation of the transgene anti-sense transcript and a reduction in the sense transcript in transgenic plants. 
     Example 18 
     Western Blot Antisense FTA Lines with Anti-FT-1 Antibodies 
     The antibodies produced according to the methods of Example 27 were used to analyze protein extracts from transgenic plants on western blots. Lane 1 of  FIG. 13  is a molecular weight standard, lane 2 purified FTA protein, lanes 3-10 are protein extracts from the ERA1 mutant, wild type, and 4 lines of transgenic  Arabidopsis thaliana .  FIG. 13  illustrates the reduction of detectable FTA protein in transgenic lines. 
     Example 19 
     ABA Sensitivity of Transgenic Seedlings 
     Seeds of wild type Columbia, era1-2 and T3 homozygous seeds of two antisense, drought tolerant lines of 35S-antisense-FTA were plated on minimum medium (½ MS) supplemented with no ABA (A), 0.3 μM (B), 0.5 μM (C) or 1.0 μM ABA (D). Plates were chilled for 3 days in 4° C. in the dark, and incubated for 11 days at 22° C. with 24 hour continuous light. era1 and transgenic lines were more inhibited in germination than wild type plants. Results are shown in  FIG. 14 . 
     Twelve day old seedling phenotypes of wild type Columbia, era1-2 and two drought tolerant 35S-antisense-FTA lines (9.9 &amp; 21.2) in minimum medium without (A) or with (B) 1 μM ABA.  FIG. 15  shows the reduced root growth and development of era1 and transgenic lines relative to wild type plants. The 35S-antisense-FTA lines show reduced root growth, similar to the era1 mutant, in response to ABA. 
     A transgenic  Brassica napus  line carrying the 35S-antisense-FTA construct was assessed for ABA sensitivity. At about 10 μm an effect was observed showing reduced seedling development and vigor at the cotyledon and first leaf stage, thereby indicating an increased sensitivity to ABA 
     ABA sensitivity is assessed in all transgenic plants engineered to have reduced or increased FTA or FTB expression or activity by the methods above. The ABA concentration used varies depending upon the species under examination. 
     Example 20 
     Drought Experiment 
     To assess the response of plants under water stress or drought one can expose plants to various situations. For example, the plant can be removed from soil or media and placed on paper towel for a period of time, such as 4 hours, then returned to a plate to continue growth and development. Survival and vigour can be assessed. 
     Alternatively one can impose a water stress in such a way as to more closely resemble a field situation by withholding water for a period of time, such as up to 6 days. Plants were grown five plants per four inch pot, in a replicated water-stress experiment. All pots were filled with equal amounts of homogeneous premixed and wetted soil. Growth conditions were 16 hour daylight (150-200 μmol/m 2 /s) at 22° C. and 70% relative humidity. On the day that the first flower opened drought treatment was initiated first by equalizing the soil water content in each pot on a weight basis and then cessation of watering. At the end of the water stress treatment plants were typically either harvested for biomass data or re-watered to complete the life cycle and determination of biomass and yield data. Physiological parameters have been assessed under stressed and optimal conditions, for example, shoot and root biomass accumulation, soil water content, water loss alone or as a function of parameters such as biomass, seed yield, and leaf number and leaf area.  FIG. 16  shows photographs of wild type Columbia (A) and four 35S-antisense-FTA transgenic  Arabidopsis thaliana  lines (B, C, D, E) after 8 days of water stress treatment. The control plant is visibly stressed and less healthy. This experiment has been conducted on transgenic lines containing vectors described by SEQ ID NO: 10, 46-64. 
     Drought or water stress tolerance is assessed in all transgenic plants engineered to have reduced or increased FTA or FTB expression or activity by the described methods. 
     Example 21 
     Analysis of Water Loss in  Arabidopsis thaliana  pRD29A-DA-FTA Lines During Drought Stress 
     Plants were grown 5 plants per 4 inch pot and 6 pots per line. When the plants had grown to the first flower stage drought treatment was initiated as described in Example 20. Pots were weighed daily and at the end of the 7 day drought treatment all plants were harvested for shoot fresh weight and dry weight determinations.  FIG. 10  shows the water loss on a per shoot dry weight basis at 4 days of water stress treatment. Of the 31 lines examined in this experiment 25 showed lower water loss relative to the Columbia wild type, 22 of which were statistically significant. All lines had been assessed for ABA sensitivity as described in Example 14, increased ABA sensitivity (ABA S ) also correlated with a decreased water loss during drought treatment. Those lines determined to have wild type ABA sensitivity (ABA WT ) were the same 6 lines (lines 2, 36, 69, 29, 24, 21) that did not show a reduced water loss compared to wild type. 
     The above experiment was repeated using two ABA S  lines, one ABA WT  line and a Columbia control. Plants were harvested after 2, 4 and 6 days of water stress treatment for shoot dry weight determinations. ABA S  transgenics had greater leaf and shoot biomass, greater soil water contents and lower water loss per shoot dry weight when compared to the ABA WT  or Columbia controls. Results were consistent at all three harvest stages. 
     The data shown in this example was obtained using transgenic plants carrying the pRD29A-DA-FTA construct. The experiment has also been conducted on lines carrying variations of this construct such as 35S-DA-FTA, pRD29A-antisense-FTA or 35S-antisense-FTA, with similar water stress tolerant trends observed. Soil water loss is assessed in all transgenic plants engineered to have reduced or increased FTA or FTB expression or activity by the described methods. 
     Example 22 
     Analysis of Shoot Fresh Weight in  Arabidopsis thaliana  pRD29A-DA-FTA Lines During Drought Stress 
     Plants were grown 5 plants per 4 inch pot and 8 pots per line. When the plants had grown to the first flower stage drought treatment was initiated as described in Example 20. Plants were re-watered after 6 days drought treatment and allowed to recover for an additional 6 days. Plants were harvested and shoot fresh weights determined.  FIG. 20  shows the shoot fresh weights. This experiment consisted of 25 transgenic lines, 2 of which are ABA WT  (line 2 and 69) and a Columbia wild type control. All 23 ABA S  transgenic lines had statistically significant greater shoot fresh weights, on average 44% greater. 
     The data shown in this example was obtained using transgenic plants carrying the pRD29A-DA-FTA construct. The experiment has been conducted on lines carrying variations of this construct such as 35S-DA-FTA, pRD29A-antisense-FTA or 35S-antisense-FTA, with similar trends observed. 
     Example 23 
     Analysis of Seed Yield in  Arabidopsis thaliana  pRD29A-DA-FTA Lines During Drought Stress and Under Optimal Conditions 
     Plants were grown 1 plant per 4 inch pot. When the plants had grown to the first flower stage drought treatment was initiated as described in Example 20. Plants were re-watered after 6 days drought treatment and allowed to grow to maturity. The optimal group was not exposed to the drought treatment. 
     Yield analysis indicates that although drought treatment results in decreased yields, the transgenics do not suffer as severely as controls and maintain a productivity advantage ( FIG. 21 ) as shown previously in Experiment 22. Comparison of the yields produced by the ABA S  transgenics versus the control plants show that a 15% greater yield was obtained under optimal conditions and a 20% increase under drought conditions. In the drought treatment group 8 of 9 transgenic lines showed greater yield than controls. Expression of yield of each line obtained under drought treatment as a percentage of its performance under optimum conditions indicates that 8 of 9 ABA S  lines outperformed the control line while 4 of 9 out performed the ABA WT  controls. 
     The data shown in this example was obtained using transgenic plants carrying the pRD29A-DA-FTA construct. The experiment has been conducted on lines carrying variations of this construct such as 35S-DA-FTA, pRD29A-antisense-FTA or 35S-antisense-FTA, with similar trends observed. 
     Example 24 
     Analysis of Vegetative Growth in  Arabidopsis thaliana  pRD29A-DA-FTA Lines Under Optimum Growth Conditions 
     Plants were grown 1 plant per 3 inch pot and 8 pots per line. Plants were harvested at three stages and fresh weights determined. Vegetative stage was defined as 14 day old seedlings, bolting stage as the appearance of first flower (19-21 day seedlings) and mid-flowering as 6 days from first flower. At each of the above stages respectively 7, 8 and 10 of the 10 ABA S  transgenic lines tested showed statistically greater shoot fresh weight biomass than the control plants ( FIG. 22 ). One Columbia line and an ABA WT  (line 2) line were used as the control group. Additionally, there was a statistically significant trend for the transgenic lines to have an increased number of rosette leaves. 
     The data shown in this example was obtained using transgenic plants carrying the pRD29A-DA-FTA construct. The experiment has been conducted on lines carrying variations of this construct such as 35S-DA-FTA, pRD29A-antisense-FTA or 35S-antisense-FTA, with similar trends observed. 
     Example 25 
     Analysis of  Arabidopsis thaliana  pRD29A-DA-FTA Lines Under Drought Treatment and Biotic Stress 
     Plants were grown 1 plant per 4 inch pot and 8 pots. When the plants had grown to the first flower stage drought treatment was initiated as described in Example 20. Plants were re-watered after 7 days drought treatment and allowed to grow to maturity. One Columbian control line (col) and one transgenic line were evaluated. Analysis of seed yield indicated less than normal yields, approximately 12% of expected optimal yield. It was determined that the soil used contained a fungal contaminant that was responsible for the reduced yields as the biotic stress could be negated by sterilization of the soil prior to use. This biotic stress was less severe in the transgenic line compared to the control which had a yield 22% of the transgenic line. In the drought treatment groups of plants the biotic stress was reduced however, transgenics outperformed controls by nearly 4.5 fold ( FIG. 23 ). 
     The data shown in this example was obtained using transgenic plants carrying the pRD29A-DA-FTA construct. The experiment has been conducted on lines carrying variations of this construct such as 35S-DA-FTA, pRD29A-antisense-FTA or 35S-antisense-FTA, with similar trends observed. 
     Example 26 
     Analysis of  Arabidopsis thaliana  pRD29A-DA-FTA Lines for Stomatal Number 
     The number of stomata on both the upper and lower surface of the leaf was assessed on two transgenic lines and a wild type Columbia control. Nail polish imprints were made of both upper and lower leaf surfaces of the fifth leaf, plants were at the early flowering stage. No differences in stoma density were observed. 
     The data shown in this example was obtained using transgenic plants carrying the pRD29A-DA-FTA construct. The experiment has been conducted on lines carrying variations of this construct such as 35S-DA-FTA, pRD29A-antisense-FTA or 35S-antisense-FTA, with similar trends observed. 
     Example 27 
     Production of Polyclonal Antibodies Against FT-A and FT-B 
     The isolated  Arabidopsis thaliana  FT sequences were cloned into the  E. coli  expression vector derived from pET11D. To generate the Histidine tagged FT-B construct the  Arabidopsis thaliana  FT-B clone and pET vector were digested with BamHI and ligated together. Restriction digests were performed to verify the orientation of the insert. To produce the FT-A construct the  Arabidopsis thaliana  FT-A clone and pET vector were digested with BamHI and EcoRI and subsequently ligated together. The resultant plasmids directed the expression of fusion proteins containing 6 consecutive histidine residues at the N-termini of AtFTA and AtFTB. The fusion proteins were expressed in the bacterial host BL21(DE3) and purified using Hi-Trap chelating chromatography as described by the manufacturer (Pharmacia). The soluble fraction of the crude bacterial extract containing the His-FT fusion proteins were loaded to a Hi-Trap column (1.5 cm×2.0 cm), and the proteins eluted with a 200 ml linear gradient of 0.0 to 0.3 M imidazole in column buffer (25 mM Tris-HCl, pH 7.5, 1 mM DTT). Fractions containing purified His-FT proteins were pooled, desalted and concentrated with a Centriprep-30 concentrator (Amicon). All purification steps were carried out at 4° C. To generate an antibody, the purified fusion protein was further separated by SDS/PAGE and the Coomassie stained band corresponding to the fusion protein was excised. Protein was eluted from the gel slice by electroelution and then emulsified in Ribi adjuvant (Ribi Immunochem) to a final volume of 1 ml. His-AtFTA or His-AtFTB (250 μg) were injected into a 3 kg New Zealand rabbit on day 1 and booster injections given on day 21 and day 35 with 200 μg of the protein. High-titer antisera were obtained one week after the final injection. These antibodies were used in the western analysis of example 18,  FIG. 13 . 
     Example 28 
     Screening for Related Genes 
     The transgenic plants of the invention can be used to identify genes which interact with the genes of the present invention. One can make use of the transgenic plants of the invention to screen for related genes, for example, suppressors, enhancers or modulators of gene expression or activity can be identified through genetic screening protocols. By way of example, a mutant library can be generated using the transgenic plants of the invention as the genetic background. Various methods are available and would be known to one of skill in the art. For example, chemical mutagens such as EMS can be used to induce point mutations in the genome, fast neutron irradiation of seeds can result in deletion mutations, T-DNA libraries can be produced that inactivate genes through insertional effects or activation tagging methods can be used to produce libraries with up-regulated genes. Analysis of these types of libraries can identify genes which rescue or modulate the phenotypes observed in the transgenic plants of the present invention. 
     Example 29 
     RT-PCR Amplification and Cloning of CaaX Prenyl Proteases 
     Total RNA was isolated from leaf tissue of  Arabidopsis thaliana, Brassica napus  and  Glycine max , using the Qiagen RNeasy kit and used as template to amplify the CPP genes by RT-PCR. Reaction conditions were as follows; IX reaction buffer (10 mM Tris-HCl pH 8.8, 1.5 mM MgCl 2 , 50 mM KCl), dNTP&#39;s at 200 μM, IpM AtCPP BamFW and AtCPP SmaRV primers, 2.5 U. Pfu DNA polymerase, and template plus water to a final volume of 100 μL. Reactions were run at 1 minute 94° C., 1 minute 60° C., 1 minute 72° C., for 30 cycles. Primers used to PCR amplify  Arabidopsis  and  Brassica  sequences were those identified by SEQ ID NO: 101 and SEQ ID NO: 102. Primers used to PCR amplify the  Glycine  sequence were those identified by SEQ ID NO: 149 and SEQ ID NO: 150. PCR products were separated from the RT-PCR reaction mixture using the Qiagen PCR column spin kit and ligated into the prepared cloning vector, pBluescript KS+. The vector had been prepared by digestion with EcoRV and treated with Taq polymerase in the presence of dTTP to produce a 3′ overhand suitable for ligation with the PCR products. The ligation products were transformed into  E. coli  DH5α cells, positive colonies selected and the resulting inserts sequenced. The above methodology is applicable to obtain homologous sequences and may require alternative primers. 
     
       
         
           
               
               
               
             
               
                 TABLE 13 
               
               
                   
               
             
            
               
                 AtCPP BamFW: 
                 5′-AAAGGATCCATGGCGAT 
                 (SEQ ID NO: 101 
               
               
                   
                 TCCTTTCATGG-3′ 
               
               
                   
               
               
                 AtCPP SmaRV: 
                 5′-AAACCCGGGTTAATCTGT 
                 (SEQ ID NO: 102) 
               
               
                   
                 CTTCTTGTCTTCTCCA-3′ 
               
               
                   
               
               
                 GmCPP SmaFW: 
                 5′-AAACCCGGGATGGCGTT 
                 (SEQ ID NO: 149) 
               
               
                   
                 TCCCTACATGGAAGCC-3′ 
               
               
                   
               
               
                 GmCPP SacRV: 
                 5′-AAAGAGCTCTTAGTCTTC 
                 (SEQ ID NO: 150) 
               
               
                   
                 CTTCTTATCCGGTTCG-3′ 
               
               
                   
               
            
           
         
       
     
     Example 30 
     Vector Construction 
     Construction of the pBI121-AtCPP construct (SEQ ID NO: 99) was prepared as follows. The pBI121 vector was digested with BamHI and SmaI The AtCPP, 1.4 kb DNA fragment from RT-PCR (SEQ ID NO: 97) was digested with BamHI and SmaI and ligated into the pBI121 vector. The GUS sequence was then removed by digestion with SmaI and EcoICR1 and the vector ligated after purification of the vector from the GUS insert to produce the pBI121-AtCPP vector ( FIG. 25A ). This construct was used to further generate constructs expressing the CPP gene from  Brassica  and  Glycine . To produce the pBI121-BnCPP construct (SEQ ID NO: 142) primer pairs identified by SEQ ID NO:101 and SEQ ID NO: 102 are used to PCR amplify the appropriate fragment which is ligated into the prepared parent vector. To produce the pBI121-GmCPP construct (SEQ ID NO: 136) primer pairs identified by SEQ ID NO: 149 and SEQ ID NO: 150 are used to PCR amplify the appropriate fragment which is ligated into the prepared parent vector. 
     Construction of the pBI121-antisense-AtCPP construct (SEQ ID NO:130). The antisense fragment was produced using PCR amplification with SEQ ID NO:97 as template and primers identified as SEQ ID NO:106 and SEQ ID NO: 107, listed in Table 14. This fragment was digested with BamHI and SmaI and used to replace the sense fragment of the pBI121-AtCPP construct (SEQ ID NO:99), to yield SEQ ID NO:130 ( FIG. 25B ). This construct, SEQ ID NO: 130, was used to further generate constructs expressing the antisense CPP gene from  Brassica  and  Glycine . To produce the pBI121-antisense-BnCPP construct (SEQ ID NO:144) primer pairs identified by SEQ ID NO:151 and SEQ ID NO:152 are used to PCR amplify the appropriate fragment which is ligated into the prepared parent vector. To produce the pBI121-antisense-GmCPP construct (SEQ ID NO:138) primer pairs identified by SEQ ID NO:153 and SEQ ID NO:154 are used to PCR amplify the appropriate fragment which is ligated into the prepared parent vector. 
     Construction of the pBI121-HP-AtCPP construct (SEQ ID NO:100). The cloning strategy involved truncating the GUS gene of pBI121 and flanking the GUS sequence with a AtCPP fragment in the antisense orientation upstream of the GUS and in the sense orientation on the downstream side of GUS. The pBI121 vector was digested with SmaI and Sad, the GUS sequence and the vector fragments were purified from one another. The isolated GUS fragment was digested using EcoRV and the 1079 bp. blunt ended EcoRVISacI fragment isolated. This was ligated back into the digested parent vector at the Small SacI sites. This intermediate vector was used in the subsequent production of the hair-pin vectors. The AtCPP fragment to be used as the gene specific hair-pin sequence was isolated by PCR. Primers identified as SEQ ID NO:103 and SEQ ID NO:104, listed in Table 14, were used to generate a 596 bp fragment. Cloning of the sense orientation fragment was achieved by digesting the PCR AtCPP fragment with Sad and ligation into the Sad site at the 3′ end of GUS. To insert the same fragment upstream of GUS, the BamHI site was opened and the ends blunted with Klenow. The PCR amplified AtCPP fragment was digested with Eco1CRI, which is an isoschizomer of Sad but leaves blunt ends, and ligated into the blunted BamHI site of the vector to yield the final construct ( FIG. 25C ). The intermediate construct used to produce SEQ ID NO:100 above contained only the truncated GUS gene and no CPP sequences this intermediate vector was used to further generate constructs expressing hair-pin CPP gene constructs from  Brassica  and  Glycine . To produce the pBI121-HP-BnCPP construct (SEQ ID NO:143) primer pairs identified by SEQ ID NO:153 and SEQ ID NO:154 are used to PCR amplify the sense fragment and primer pairs identified by SEQ ID NO:155 and SEQ ID NO:156 are used to PCR amplify the antisense fragment. These fragments are cloned into the prepared intermediate vector described above. To produce the pBI121-HP-GmCPP construct (SEQ ID NO:137) primer pairs identified by SEQ ID NO:157 and SEQ ID NO:158 are used to PCR amplify the sense fragment and primer pairs identified by SEQ ID NO:159 and SEQ ID NO:160 are used to PCR amplify the antisense fragment. These fragments are cloned into the prepared intermediate vector described above. 
     The above vector constructs were modified to place the genes under the control of alternative promoters, such as, but not limited to, the RD29A or MuA. This was accomplished by excising the 35S promoter sequence and replacing it with an appropriate promoter sequence. In this way SEQ ID NO&#39;s: 134 and 135 were generated and SEQ ID NO&#39;s: 133, 136-148 can be constructed. 
     
       
         
           
               
               
               
             
               
                 TABLE 14 
               
               
                   
               
             
            
               
                 AtCPP-HP-SacFW 
                 5′-CTGGAGCTCT 
                 (SEQ ID NO: 103) 
               
               
                   
                 TTTACCGAGGTT 
               
               
                   
                 GGGCCTTGATCC-3′ 
               
               
                   
               
               
                 AtCPP-HP-SacRV 
                 5′-ATTGAGCTCCC 
                 (SEQ ID NO: 104) 
               
               
                   
                 AATGTCCAAGCT 
               
               
                   
                 CGTGTGCAATA-3′ 
               
               
                   
               
               
                 AtCPP-anti-SmaFW 
                 5′-AAACCCGGGATGG 
                 (SEQ ID NO: 106) 
               
               
                   
                 CGATTCCTTTCATGG-3′ 
               
               
                   
               
               
                 AtCPP-anti-BamRV 
                 5′-AAAGGATCCTTAA 
                 (SEQ ID NO: 107) 
               
               
                   
                 TCTGTCTTCTT 
               
               
                   
                 GTCTTCTCCA-3′ 
               
               
                   
               
               
                 BnCPP-anti-SmaFW 
                 5′-AAACCCGGGAT 
                 (SEQ ID NO: 151) 
               
               
                   
                 GGCGATTCCT 
               
               
                   
                 TTCATGG-3′ 
               
               
                   
               
               
                 BnCPP-anti-BamRV 
                 5′-AAAGGATCCTTA 
                 (SEQ ID NO: 152) 
               
               
                   
                 ATCTGTCTTCTT 
               
               
                   
                 GTCTTCTCC-3′ 
               
               
                   
               
               
                 BnCPP-HP-Sac-FW 
                 5′-AAAGAGCTCTTCTAC 
                 (SEQ ID NO: 153) 
               
               
                   
                 CAATGGTGGGACTCG-3′ 
               
               
                   
               
               
                 BnCPP-HP-Sac-RV 
                 5′-AAAGAGCTCCCAGTG 
                 (SEQ ID NO: 154) 
               
               
                   
                 TCCCAGCTCGTGTG-3′ 
               
               
                   
               
               
                 BnCPP-HP-BamFW 
                 5′-AAAGGATCCTTCTAC 
                 (SEQ ID NO: 155) 
               
               
                   
                 CAATGGTGGGACTCG-3′ 
               
               
                   
               
               
                 BnCPP-HP-XbaRV 
                 5′-AAATCTAGACCAGTG 
                 (SEQ ID NO: 156) 
               
               
                   
                 TCCCAGCTCGTGTG-3′ 
               
               
                   
               
               
                 GmCPP-HP-Sac-FW 
                 5′-GATGAGCTCACAA 
                 (SEQ ID NO: 157) 
               
               
                   
                 GATCAAGTCAC 
               
               
                   
                 AGCAATGCCT-3′ 
               
               
                   
               
               
                 GmCPP-HP-Sac-RV 
                 5′-AAAGAGCTCCCGGTT 
                 (SEQ ID NO: 158) 
               
               
                   
                 CGTCCAGCGCGGCC-3′ 
               
               
                   
               
               
                 GmCPP-HP-BamFW 
                 5′-GATGGATCCACAA 
                 (SEQ ID NO: 159) 
               
               
                   
                 GATCAAGTCACA 
               
               
                   
                 GCAATGCCT-3′ 
               
               
                   
               
               
                 GmCPP-HP-XbaRV 
                 5′-CCTTCTAGACCGGTT 
                 (SEQ ID NO: 160) 
               
               
                   
                 CGTCCAGCGCGGCC-3′ 
               
               
                   
               
            
           
         
       
     
     Example 31 
     Sequence Analysis 
       Arabidopsis thaliana  CPP (AtCPP) 
     A disclosed nucleic acid of 1275 nucleotides (SEQ ID NO:97) and also referred to as AtCPP, is shown in Table 15. 
                     TABLE 15A               AtCPP Nucleotide Sequence (SEQ ID NO: 97).                                    ATGGCGATTCCTTTCATGGAAACCGTCGTGGGTTTTATGATA               GTGATGTACATTTTTGAGACGTATTTGGATCTGAGGCAACTC               ACTGCTCTCAAGCTTCCAACTCTCCCGAAAACCTTGGTTGG               TGTAATTAGCCAAGAGAAGTTTGAGAAATCACGAGCATAC               AGTCTTGACAAAAGCTATTTTCACTTTGTTCATGAGTTTGTA               ACTATACTTATGGACTCTGCAATTTTGTTCTTTGGGATCTTG               CCTTGGTTTTGGAAGATGTCTGGAGCTGTTTTACCGAGGTT               GGGCCTTGATCCGGAGAATGAAATACTGCATACTCTTTCATT               CTTGGCTGGTGTTATGACATGGTCACAGATCACTGATTTGCCA               TTTTCTTTGTACTCAACTTTCGTGATCGAGTCTCGGCATGGGTTC               AACAAACAAACAATATGGATGTTCATTAGGGACATGATCAAAGGAACA               TTCCTCTCTGTCATACTAGGCCCACCCATTGTTGCTGCGATAAT               TTTCATAGTCCAGAAAGGAGGTCCTTATCTTGCCATCTATCTGTG               GGCATTCATGTTTATCCTGTCTCTAGTGATGATGACTATATACCCG               GTCTTGATAGCACCGCTCTTCAACAAATTCACTCCTCTTCCAGATG               GAGACCTCCGGGAGAAGATTGAGAAACTTGCTTCTTCCCTAAAGTT               TCCTTTGAAGAAGCTGTTTGTTGTCGATGGATCTACAAGGTCAAGCC               ATAGCAATGCTTACATGTATGGTTTCTTTAAGAACAAAAGGATTGTTC               TTTATGATACGTTGATTCAGCAGTGCAAGAATGAGGATGAAATTGTGGCG               GTTATTGCACACGAGCTTGGACATTGGAAACTGAATCACACTACATACTC               GTTCATTGCAGTTCAAATCCTTGCCTTCTTACAATTTGGAGGATACACT               CTTCTCAGAAACTCCACTGATCTCTTCAGGAGTTTCGGATTTGATACAC               AGCCTGTTCTCATTGGTTTGATCATATTTCAGCACACTGTAATACCACTG               CAACATCTAGTAAGCTTTGGCCTGAACCTCGTTAGTCGAGCGTTTGAGT               TTCAGGCTGATGCTTTTGCTGTGAAGCTTGACTATGCAAAAGATCTT               CGTCCTGCTCTAGTGAAACTACAGGAAGAGAACTTATCAACAATGAAC               ACTGATCCATTGTACTCAGCTTATCACTACTCACATCCTCCTCTTGTTG               AAAGGCTTCGAGCCACTGATGGAGAAGAC AAGAAGACAGATTAA                      
A disclosed CPP polypeptide (SEQ ID NO:98) encoded by SEQ ID NO:97 has 424 amino acid residues and is presented in Table 15B using the one-letter amino acid code.
 
     
       
         
           
               
             
               
                 TABLE 15B 
               
               
                   
               
               
                 Encoded CPP protein sequence (SEQ ID NO: 98). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 MAIPFMETVVGFMIVMYIFETYLDLRQLTALKLPTLPKTLVGVISQE 
               
               
                   
               
               
                 KFEKSRAYSLDKSYFHFVHEFVTILMDSAILFFGILPWFWKMSGAV 
               
               
                   
               
               
                 LPRLGLDPENEILHTLSFLAGVMTWSQITDLPFSLYSTFVIESRHGF 
               
               
                   
               
               
                 NKQTIWMFIRDMIKGTFLSVILGPPIVAAIIFIVQKGGPYLAIYLWAFM 
               
               
                   
               
               
                 FILSLVMMTIYPVLIAPLFNKFTPLPDGDLREKIEKLASSLKFPLKKL 
               
               
                   
               
               
                 FVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNEDEIVAVIAH 
               
               
                   
               
               
                 ELGHWKLNHTTYSFIAVQILAFLQFGGYTLLRNSTDLFRSFGFDTQP 
               
               
                   
               
               
                 VLIGLIIFQHTVIPLQHLVSFGLNLVSRAFEFQADAFAVKLDYAKDLRP 
               
               
                   
               
               
                 ALVKLQEENLSTMNTDPLYSAYHYSHPPLVERLRATDGEDKKTD 
               
               
                   
               
            
           
         
       
     
     The present invention also includes a nucleic acid sequence complimentary to the  Arabidopsis thaliana  CaaX prenyl protease of SEQ ID NO:97. The disclosed complimentary sequence is shown as SEQ ID NO:115. 
     
       
         
           
               
            
               
                 SEQ ID NO: 115 
               
            
           
           
               
            
               
                 TTAATCTGTCTTCTTGTCTTCTCCATCAGTGGCTCGAAGCCTTTC 
               
               
                   
               
               
                 AACAAGAGGAGGATGTGAGTAGTGATAAGCTGAGTACAATGGAT 
               
               
                   
               
               
                 CAGTGTTCATTGTTGATAAGTTCTCTTCCTGTAGTTTCACTAGAGC 
               
               
                   
               
               
                 AGGACGAAGATCTTTTGCATAGTCAAGCTTCACAGCAAAAGCATC 
               
               
                   
               
               
                 AGCCTGAAACTCAAACGCTCGACTAACGAGGTTCAGGCCAAAGC 
               
               
                   
               
               
                 TTACTAGATGTTGCAGTGGTATTACAGTGTGCTGAAATATGATCAAA 
               
               
                   
               
               
                 CCAATGAGAACAGGCTGTGTATCAAATCCGAAACTCCTGAAGAGAT 
               
               
                   
               
               
                 CAGTGGAGTTTCTGAGAAGAGTGTATCCTCCAAATTGTAAGAAGGC 
               
               
                   
               
               
                 AAGGATTTGAACTGCAATGAACGAGTATGTAGTGTGATTCAGTTTCCA 
               
               
                   
               
               
                 ATGTCCAAGCTCGTGTGCAATAACCGCCACAATTTCATCCTCATTCTT 
               
               
                   
               
               
                 GCACTGCTGAATCAACGTATCATAAAGAACAATCCTTTTGTTCTTAAAG 
               
               
                   
               
               
                 AAACCATACATGTAAGCATTGCTATGGCTTGACCTTGTAGATCCATCG 
               
               
                   
               
               
                 ACAACAAACAGCTTCTTCAAAGGAAACTTTAGGGAAGAAGCAAGTTTC 
               
               
                   
               
               
                 TCAATCTTCTCCCGGAGGTCTCCATCTGGAAGAGGAGTGAATTTGTTGA 
               
               
                   
               
               
                 AGAGCGGTGCTATCAAGACCGGGTATATAGTCATCATCACTAGAGACA 
               
               
                   
               
               
                 GGATAAACATGAATGCCCACAGATAGATGGCAAGATAAGGACCTCCTT 
               
               
                   
               
               
                 TCTGGACTATGAAAATTATCGCAGCAACAATGGGTGGGCCTAGTATGAC 
               
               
                   
               
               
                 AGAGAGGAATGTTCCTTTGATCATGTCCCTAATGAACATCCATATTGTTT 
               
               
                   
               
               
                 GTTTGTTGAACCCATGCCGAGACTCGATCACGAAAGTTGAGTACAAAG 
               
               
                   
               
               
                 AAAATGGCAAATCAGTGATCTGTGACCATGTCATAACACCAGCCAAGAAT 
               
               
                   
               
               
                 GAAAGAGTATGCAGTATTTCATTCTCCGGATCAAGGCCCAACCTCGGTAA 
               
               
                   
               
               
                 AACAGCTCCAGACATCTTCCAAAACCAAGGCAAGATCCCAAAGAACAAA 
               
               
                   
               
               
                 ATTGCAGAGTCCATAAGTATAGTTACAAACTCATGAACAAAGTGAAAATA 
               
               
                   
               
               
                 GCTTTTGTCAAGACTGTATGCTCGTGATTTCTCAAACTTCTCTTGGCTAA 
               
               
                   
               
               
                 TTACACCAACCAAGGTTTTCGGGAGAGTTGGAAGCTTGAGAGCAGTGAG 
               
               
                   
               
               
                 TTGCCTCAGATCCAAATACGTCTCAAAAATGTACATCACTATCATAAAAC 
               
               
                   
               
               
                 CCACGACGGTTTCCATGAAAGGAATCGCCAT 
               
            
           
         
       
     
     Due to the nature of the cloning strategy the sequence presented is not full length but is missing the 5′ and 3′ non-translated regions. The percent identities of the  Arabidopsis thaliana  nucleotide sequence and its encoded amino acid sequence to that of other CPP sequences as determined by ClustalW analysis are shown in  FIG. 26 . 
     Using the sequences disclosed herein as hybridization probes, one is able to screen and isolate full length sequences from cDNA or genomic libraries or use the rapid amplification of cDNA ends (RACE) technology or other such PCR techniques. 
       Brassica napus  CPP (BnCPP) 
     A disclosed nucleic acid of 1275 nucleotides (SEQ ID NO:109) and also referred to as BnCPP, is shown in Table 16. 
     
       
         
           
               
             
               
                 TABLE 16A 
               
               
                   
               
               
                 BnCPP Nucleotide Sequence (SEQ ID NO: 109). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 ATGGCGATTCCTTTCATGGAAACCGTCGTTGGTTTTATGATAGTGATGTA 
               
               
                   
               
               
                 CGTTTTTGAGACGTATTTGGATCTGAGGCAACATACTGCTCTCAAGCTTC 
               
               
                   
               
               
                 CCACTCTCCCAAAGACTTTGGTTGGAGTCATTAGCCAAGAGAAGTTTGAG 
               
               
                   
               
               
                 AAATCTCGAGCTTACAGTCTTGACAAAAGCCATTTTCACTTTGTTCATGA 
               
               
                   
               
               
                 GTTTGTTACTATACTTATGGACTCTGCGATTCTGTTCTTTGGGATCTTG 
               
               
                   
               
               
                 CCTTGGTTTTGGAAGATATCTGGCGGCTTTCTACCAATGGTGGGACTCG 
               
               
                   
               
               
                 ATCCAGAGAATGAAATCCTGCACACTCTTTCATTCTTGGCTGGTCTTATG 
               
               
                   
               
               
                 ACATGGTCACAGATCACTGATTTGCCATTTTCTTTGTACTCAACTTTCGT 
               
               
                   
               
               
                 GATCGAGTCTCGGCATGGGTTCAACAAACAAACAATATGGATGTTCATTA 
               
               
                   
               
               
                 GGGACATGATCAAAGGAATACTCCTCTCTGTCATACCTGCCCCTCCTATC 
               
               
                   
               
               
                 GTTGCCGCAATTATTGTTATAGTTCAGAAAGGAGGTCCTTACCTCGCCAT 
               
               
                   
               
               
                 CTATCTGTGGGCATTCATGTTTATCCTGTCTCTAGTGATGATGACTATAT 
               
               
                   
               
               
                 ACCCTGTTTTGATTGCACCTCTTTTCAACAAGTTCACTCCTCTTCCTGAT 
               
               
                   
               
               
                 GGAGACCTCCGGGAGAAGATTGAGAAACTTGCTTCTTCTCTAAAGTTTCC 
               
               
                   
               
               
                 TCTGAAGAAGCTGTTTGTTGTCGATGGATCTACAAGGTCAAGCCATAGTA 
               
               
                   
               
               
                 ATGCTTACATGTATGGTTTCTTCAAGAACAAAAGGATTGTTCTTTATGAC 
               
               
                   
               
               
                 ACATTGATTCAGCAGTGCCAGAATGAGAATGAAATTGTGGCGGTTATTGC 
               
               
                   
               
               
                 ACACGAGCTGGGACACTGGAAGCTGAATCACACTACATACTCGTTCATTG 
               
               
                   
               
               
                 CTGTTCAAATCCTTGCCTTCTTGCAATTTGGAGGATACACTCTTGTCAGA 
               
               
                   
               
               
                 AACTCCACTGATCTCTTCAGGAGTTTTGGTTTTGATACACAACCAGTTCT 
               
               
                   
               
               
                 CATTGGTTTGATCATATTTCAGCACACTGTAATACCACTTCAACACCTAG 
               
               
                   
               
               
                 TAAGCTTTGACCTCAACCTTGTTAGTCGAGCGTTTGAGTTTCAGGCTGA 
               
               
                   
               
               
                 TGCTTTTGCAGTGAATCTTGGTTATGCAAAGGATCTACGTCCTGCCCTA 
               
               
                   
               
               
                 GTGAAGCTACAGGAAGAGAACTTATCAGCGATGAACACAGACCCATTG 
               
               
                   
               
               
                 TACTCAGCTTATCACTACTCACACCCTCCTCTTGTAGAGAGGCTTCGA 
               
               
                   
               
               
                 GCCATTGATGGAGAAGACAAGAAGACAGATTAA 
               
               
                   
               
            
           
         
       
     
     A disclosed CPP polypeptide (SEQ ID NO:110) encoded by SEQ ID NO:109 has 424 amino acid residues and is presented in Table 16B using the one-letter amino acid code. 
     
       
         
           
               
             
               
                 TABLE 16B 
               
               
                   
               
               
                 Encoded CPP protein sequence (SEQ ID NO: 110). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 MAIPFMETVVGFMIVMYVFETYLDLRQHTALKLPTLPKTLVGVISQEKFE 
               
               
                   
               
               
                 KSRAYSLDKSHFHFVHEFVTILMDSAILFFGILPWFWKISGGFLPMVGLD 
               
               
                   
               
               
                 PENEILHTLSFLAGLMTWSQITDLPFSLYSTFVIESRHGFNKQTIWMFIR 
               
               
                   
               
               
                 DMIKGILLSVIPAPPIVAAIIVIVQKGGPYLAIYLWAFMFILSLVMMTIY 
               
               
                   
               
               
                 PVLIAPLFNKFTPLPDGDLREKIEKLASSLKFPLKKLFVVDGSTRSSHSN 
               
               
                   
               
               
                 AYMYGFFKNKRIVLYDTLIQQCQNENEIVAVIAHELGHWKLNHTTYSFIA 
               
               
                   
               
               
                 VQILAFLQFGGYTLVRNSTDLFRSFGFDTQPVLIGLIIFQHTVIPLQHLV 
               
               
                   
               
               
                 SFDLNLVSRAFEFQADAFAVNLGYAKDLRPALVKLQEENLSAMNTDPLYS 
               
               
                   
               
               
                 AYHYSHPPLVERLRAIDGEDKKTD 
               
               
                   
               
            
           
         
       
     
     The present invention also includes a nucleic acid sequence complimentary to the  Brassica napus  CaaX prenyl protease of SEQ ID NO:109. The disclosed complimentary sequence is shown as SEQ ID NO:111. 
     
       
         
           
               
            
               
                 SEQ ID NO: 111 
               
            
           
           
               
            
               
                 TTAATCTGTCTTCTTGTCTTCTCCATCAATGGCTCGAAGCCTCTCTAC 
               
               
                   
               
               
                 AAGAGGAGGGTGTGAGTAGTGATAAGCTGAGTACAATGGGTCTGTGT 
               
               
                   
               
               
                 TCATCGCTGATAAGTTCTCTTCCTGTAGCTTCACTAGGGCAGGACGTA 
               
               
                   
               
               
                 GATCCTTTGCATAACCAAGATTCACTGCAAAAGCATCAGCCTGAAACTC 
               
               
                   
               
               
                 AAACGCTCGACTAACAAGGTTGAGGTCAAAGCTTACTAGGTGTTGAAG 
               
               
                   
               
               
                 TGGTATTACAGTGTGCTGAAATATGATCAAACCAATGAGAACTGGTTGT 
               
               
                   
               
               
                 GTATCAAAACCAAAACTCCTGAAGAGATCAGTGGAGTTTCTGACAAGAG 
               
               
                   
               
               
                 TGTATCCTCCAAATTGCAAGAAGGCAAGGATTTGAACAGCAATGAACG 
               
               
                   
               
               
                 AGTATGTAGTGTGATTCAGCTTCCAGTGTCCCAGCTCGTGTGCAATAA 
               
               
                   
               
               
                 CCGCCACAATTTCATTCTCATTCTGGCACTGCTGAATCAATGTGTCAT 
               
               
                   
               
               
                 AAAGAACAATCCTTTTGTTCTTGAAGAAACCATACATGTAAGCATTACT 
               
               
                   
               
               
                 ATGGCTTGACCTTGTAGATCCATCGACAACAAACAGCTTCTTCAGAGG 
               
               
                   
               
               
                 AAACTTTAGAGAAGAAGCAAGTTTCTCAATCTTCTCCCGGAGGTCTCC 
               
               
                   
               
               
                 ATCAGGAAGAGGAGTGAACTTGTTGAAAAGAGGTGCAATCAAAACAG 
               
               
                   
               
               
                 GGTATATAGTCATCATCACTAGAGACAGGATAAACATGAATGCCCACA 
               
               
                   
               
               
                 GATAGATGGCGAGGTAAGGACCTCCTTTCTGAACTATAACAATAATTGC 
               
               
                   
               
               
                 GGCAACGATAGGAGGGGCAGGTATGACAGAGAGGAGTATTCCTTTGAT 
               
               
                   
               
               
                 CATGTCCCTAATGAACATCCATATTGTTTGTTTGTTGAACCCATGCCGAG 
               
               
                   
               
               
                 ACTCGATCACGAAAGTTGAGTACAAAGAAAATGGCAAATCAGTGATCTGT 
               
               
                   
               
               
                 GACCATGTCATAAGACCAGCCAAGAATGAAAGAGTGTGCAGGATTTCATT 
               
               
                   
               
               
                 CTCTGGATCGAGTCCCACCATTGGTAGAAAGCCGCCAGATATCTTCCAAA 
               
               
                   
               
               
                 ACCAAGGCAAGATCCCAAAGAACAGAATCGCAGAGTCCATAAGTATAGTA 
               
               
                   
               
               
                 ACAAACTCATGAACAAAGTGAAAATGGCTTTTGTCAAGACTGTAAGCTCG 
               
               
                   
               
               
                 AGATTTCTCAAACTTCTCTTGGCTAATGACTCCAACCAAAGTCTTTGGGA 
               
               
                   
               
               
                 GAGTGGGAAGCTTGAGAGCAGTATGTTGCCTCAGATCCAAATACGTCTC 
               
               
                   
               
               
                 AAAAACGTACATCACTATCATAAAACCAACGACGGTTTCCATGAAAGGA 
               
               
                   
               
               
                 ATCGCCAT 
               
            
           
         
       
     
     Due to the nature of the cloning strategy the sequence presented is not full length but is missing the 5′ and 3′ non-translated regions. The percent identities of the  Brassica napus  nucleotide sequence and its encoded amino acid sequence to that of other CPP sequences as determined by ClustalW analysis are shown in  FIG. 26 . 
     Using the sequences disclosed herein as hybridization probes, one is able to screen and isolate full length sequences from cDNA or genomic libraries or use the rapid amplification of cDNA ends (RACE) technology or other such PCR techniques. 
       Glycine max  CPP (GmCPP) 
     A disclosed nucleic acid of 1275 nucleotides (SEQ ID NO:112) and also referred to as GmCPP, is shown in Table 17. 
     
       
         
           
               
             
               
                 TABLE 17A 
               
               
                   
               
               
                 GmCPP Nucleotide Sequence (SEQ ID NO: 112). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 ATGGCGTTTCCCTACATGGAAGCCGTTGTCGGATTTATGATATTAATGTA 
               
               
                   
               
               
                 CATTTTTGAAACTTACTTGGATGTGCGACAACATAGGGCCCTCAAAC 
               
               
                   
               
               
                 TTCCTACTCTTCCAAAGACTTTAGAGGGTGTTATCAGCCAAGAGAAA 
               
               
                   
               
               
                 TTTGAGAAATCTAGAGCCTATAGTCTTGATAAAAGCCACTTCCATTTT 
               
               
                   
               
               
                 GTTCACGAGTTTGTGACAATAGTGACAGACTCTACAATTTTGTACTTT 
               
               
                   
               
               
                 GGGGTATTGCCCTGGTTTTGGAAGAAATCAGGAGATTTTATGACAATA 
               
               
                   
               
               
                 GCTGGTTTCAATGCTGAGAATGAAATACTGCATACCCTTGCCTTCTTA 
               
               
                   
               
               
                 GCAGGGCTGATGATTTGGTCACAGATAACAGATTTGCCCTTTTCTCTG 
               
               
                   
               
               
                 TACTCAACTTTTGTGATTGAGGCCCGTCATGGTTTTAATAAGCAAACA 
               
               
                   
               
               
                 CCATGGTTATTCTTTAGGGACATGCTTAAAGGAATTTTCCTTTCTGTAA 
               
               
                   
               
               
                 TAATTGGTCCACCTATTGTGGCTGCAATCATTGTAATAGTACAGAAAG 
               
               
                   
               
               
                 GAGGTCCATACTTGGCCATCTATCTTTGGGTTTTTACGTTTGGTCTTT 
               
               
                   
               
               
                 CTATTGTGATGATGACCCTTTATCCAGTACTAATAGCTCCACTCTTCA 
               
               
                   
               
               
                 ATAAGTTCACTCCACTTCCAGATGGTCAACTCAGGGAGAAAATCGAG 
               
               
                   
               
               
                 AAACTTGCTTCCTCCCTCAACTATCCGTTAAAGAAACTATTTGTTGTCG 
               
               
                   
               
               
                 ATGGATCCACAAGATCAAGTCACAGCAATGCCTATATGTATGGATTCT 
               
               
                   
               
               
                 TCAAGAACAAGAGGATTGTCCCTTATGACACATTAATTCAACAGTGCA 
               
               
                   
               
               
                 AAGACGATGAGGAAATTGTTGCTGTTATTGCCCATGAGTTGGGACACTG 
               
               
                   
               
               
                 GAAGCTCAACCATACTGTGTACACATTTGTTGCTATGCAGATTCTTACAC 
               
               
                   
               
               
                 TTCTACAATTTGGAGGATATACACTAGTGCGAAATTCAGCTGATCTGTA 
               
               
                   
               
               
                 TCGAAGCTTTGGGTTTGATACGCAGCCAGTCCTCATTGGGCTCATCATA 
               
               
                   
               
               
                 TTTCAGCATACTGTAATCCCACTTCAGCAATTGGTCAGCTTTGGTCTGAA 
               
               
                   
               
               
                 CCTAGTCAGCCGATCATTTGAATTTCAGGCTGATGGCTTTGCCAAGAAG 
               
               
                   
               
               
                 CTTGGATATGCATCTGGATTACGCGGTGGTCTTGTGAAACTACAGGAGG 
               
               
                   
               
               
                 AGAATCTGTCAGCTATGAATACAGATCCTTGGTACTCTGCTTATCACTAT 
               
               
                   
               
               
                 TCTCATCCTCCCCTTGTTGAAAGATTGGCCGCGCTGGACGAACCGGAT 
               
               
                   
               
               
                 AAGAAGGAAGACTAA 
               
               
                   
               
            
           
         
       
     
     A disclosed CPP polypeptide (SEQ ID NO:113) encoded by SEQ ID NO:112 has 424 amino acid residues and is presented in Table 17B using the one-letter amino acid code. 
     
       
         
           
               
             
               
                 TABLE 17B 
               
               
                   
               
               
                 Encoded CPP protein sequence (SEQ ID NO: 113). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 MAFPYMEAVVGFMILMYIFETYLDVRQHRALKLPTLPKTLEGVISQEKFE 
               
               
                   
               
               
                 KSRAYSLDKSHFHFVHEFVTIVTDSTILYFGVLPWFWKKSGDFMTIAGF 
               
               
                   
               
               
                 NAENEILHTLAFLAGLMIWSQITDLPFSLYSTFVIEARHGFNKQTPWLFF 
               
               
                   
               
               
                 RDMLKGIFLSVIIGPPIVAAIIVIVQKGGPYLAIYLWVFTFGLSIVMMT 
               
               
                   
               
               
                 LYPVLIAPLFNKFTPLPDGQLREKIEKLASSLNYPLKKLFVVDGSTRS 
               
               
                   
               
               
                 SHSNAYMYGFFKNKRIVPYDTLIQQCKDDEEIVAVIAHELGHWKLN 
               
               
                   
               
               
                 HTVYTFVAMQILTLLQFGGYTLVRNSADLYRSFGFDTQPVLIGLIIFQ 
               
               
                   
               
               
                 HTVIPLQQLVSFGLNLVSRSFEFQADGFAKKLGYASGLRGGLVKL 
               
               
                   
               
               
                 QEENLSAMNTDPWYSAYHYSHPPLVERLAALDEPDKKED 
               
               
                   
               
            
           
         
       
     
     The present invention also includes a nucleic acid sequence complimentary to the  Glycine max  CaaX prenyl protease of SEQ ID NO:112. The disclosed complimentary sequence is shown as SEQ ID NO:114. 
     
       
         
           
               
            
               
                 SEQ ID NO: 114 
               
               
                 TTAGTCTTCCTTCTTATCCGGTTCGTCCAGCGCGGCCAATCTTTCAACA 
               
               
                   
               
               
                 AGGGGAGGATGAGAATAGTGATAAGCAGAGTACCAAGGATCTGTATT 
               
               
                   
               
               
                 CATAGCTGACAGATTCTCCTCCTGTAGTTTCACAAGACCACCGCGTA 
               
               
                   
               
               
                 ATCCAGATGCATATCCAAGCTTCTTGGCAAAGCCATCAGCCTGAAA 
               
               
                   
               
               
                 TTCAAATGATCGGCTGACTAGGTTCAGACCAAAGCTGACCAATTGC 
               
               
                   
               
               
                 TGAAGTGGGATTACAGTATGCTGAAATATGATGAGCCCAATGAGGA 
               
               
                   
               
               
                 CTGGCTGCGTATCAAACCCAAAGCTTCGATACAGATCAGCTGAAT 
               
               
                   
               
               
                 TTCGCACTAGTGTATATCCTCCAAATTGTAGAAGTGTAAGAATCTGC 
               
               
                   
               
               
                 ATAGCAACAAATGTGTACACAGTATGGTTGAGCTTCCAGTGTCCCA 
               
               
                   
               
               
                 ACTCATGGGCAATAACAGCAACAATTTCCTCATCGTCTTTGCACTGT 
               
               
                   
               
               
                 TGAATTAATGTGTCATAAGGGACAATCCTCTTGTTCTTGAAGAATCCA 
               
               
                   
               
               
                 TACATATAGGCATTGCTGTGACTTGATCTTGTGGATCCATCGACAAC 
               
               
                   
               
               
                 AAATAGTTTCTTTAACGGATAGTTGAGGGAGGAAGCAAGTTTCTCGA 
               
               
                   
               
               
                 TTTTCTCCCTGAGTTGACCATCTGGAAGTGGAGTGAACTTATTGAAG 
               
               
                   
               
               
                 AGTGGAGCTATTAGTACTGGATAAAGGGTCATCATCACAATAGAAA 
               
               
                   
               
               
                 GACCAAACGTAAAAACCCAAAGATAGATGGCCAAGTATGGACCTCC 
               
               
                   
               
               
                 TTTCTGTACTATTACAATGATTGCAGCCACAATAGGTGGACCAATT 
               
               
                   
               
               
                 ATTACAGAAAGGAAAATTCCTTTAAGCATGTCCCTAAAGAATAACCA 
               
               
                   
               
               
                 TGGTGTTTGCTTATTAAAACCATGACGGGCCTCAATCACAAAAGTTG 
               
               
                   
               
               
                 AGTACAGAGAAAAGGGCAAATCTGTTATCTGTGACCAAATCATCAG 
               
               
                   
               
               
                 CCCTGCTAAGAAGGCAAGGGTATGCAGTATTTCATTCTCAGCATTG 
               
               
                   
               
               
                 AAACCAGCTATTGTCATAAAATCTCCTGATTTCTTCCAAAACCAGG 
               
               
                   
               
               
                 GCAATACCCCAAAGTACAAAATTGTAGAGTCTGTCACTATTGTCAC 
               
               
                   
               
               
                 AAACTCGTGAACAAAATGGAAGTGGCTTTTATCAAGACTATAGGCT 
               
               
                   
               
               
                 CTAGATTTCTCAAATTTCTCTTGGCTGATAACACCCTCTAAAGTCTT 
               
               
                   
               
               
                 TGGAAGAGTAGGAAGTTTGAGGGCCCTATGTTGTCGCACATCCAA 
               
               
                   
               
               
                 GTAAGTTTCAAAAATGTACATTAATATCATAAATCCGACAACGGCT 
               
               
                   
               
               
                 TCCATGTAGGGAAACGCCAT 
               
            
           
         
       
     
     Due to the nature of the cloning strategy the sequence presented is not full length but is missing the 5′ and 3′ non-translated regions. The percent identities of the  Glycine max  nucleotide sequence and its encoded amino acid sequence to that of other CPP sequences as determined by ClustalW analysis are shown in  FIG. 26 . 
     Using the sequences disclosed herein as hybridization probes, one is able to screen and isolate full length sequences from cDNA or genomic libraries or use the rapid amplification of cDNA ends (RACE) technology or other such PCR techniques. 
     The CPP nucleic acids and amino acids disclosed above have homology to other disclosed CPP sequences (GenBank ID NOs: AL161491 (AT4g01320), AF007269 and AF353722; WO 02/16625 A2). The homology between these and other sequences is shown in the ClustalW alignment analysis shown in Tables 18A-18B. 
     
       
         
           
               
             
               
                 TABLE 18A 
               
               
                   
               
               
                 ClustalW Nucleic Acid Analysis of CaaX Prenyl Protease 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 1: PPI-AtCPP 
                 SEQ ID NO: 97 
               
               
                   
               
               
                 2: PPI-BnCPP 
                 SEQ ID NO: 109 
               
               
                   
               
               
                 3: PPI-GmCPP 
                 SEQ ID NO: 112 
               
               
                   
               
               
                 4: BASF_AT1 
                 SEQ ID NO: 116 
               
               
                   
               
               
                 5: BASF_AT2 
                 SEQ ID NO: 118 
               
               
                   
               
               
                 6: BASF-Corn 
                 SEQ ID NO: 120 
               
               
                   
               
               
                 7: BASF-Gm 
                 SEQ ID NO: 122 
               
               
                   
               
               
                 8: AFC1 
                 SEQ ID NO: 124 
               
               
                   
               
               
                 9: AT4g01320 
                 SEQ ID NO: 126 
               
               
                   
               
               
                 10: AF007269 
                 SEQ ID NO: 128 
               
               
                   
               
            
           
           
               
            
               
                 CLUSTAL W (1.81) multiple sequence alignment 
               
               
                   
               
            
           
           
               
               
            
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 ATGGCGATTCCTTTCATGGAAACCGTCGTGGGTAAGCTTCAAAACCTTTTTCTGAGACAT 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 TTTACTATCCTGTTTCACTCATCGTATTTCGTTTTTGTTTGGGTTTTGCTTTCTGTGTTG 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 TGTGTGTTGAGATTCCATGACTCGTTTGTTTCATATACCATCGTCTCTGCTTCTCGTTTC 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 TAAATTTTGTTCTTTTCTAATAGTGCGTACCTTGATCTGAGGTTTTATTACTCCTACTAG 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 TTTCTTGTCTTACTCGTGCGTTTGATTTGATTTGAGCTTATGTGATTTCATCATCTCTTC 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 CTCGGTTTTAGAATGTACGGAGCTTCTCTGTTAACCAAAATCTAGGATTTGGGAAGAAAA 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 GTCGGAGTCTTTTTTTTCCTCATTCCCGATTGGAAATTGAGAATCTTGAAATTTTTCTTT 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 --------------------------------------CTAATACGACTCACTATAGGGC 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 GTTCAAGTCATACAGCTTGAGGTTTTGGGTTTTCTTGTCAGGGTATTATTATGTTCGTGA 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 AAGCAGTGGTAACAACGCAGAGTACGCGGGGGGAGACGCATGGTTCTGAACTAATTGTTA 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 CTGCAACTAGAGTTTTCTGGAGTTTTTTGAAATGGGTTTTGTGTTGTGGAACCGTATGTG 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 TAAATAATACCTAAAATTTTGAGTTGTCCTAAACATTGGGGTTTAAACAAATCCAATCTC 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 AATGTTGCATCAAAACTCTTTCAGTGCTCCAATGTTTCCATCAGTAGTCAGCACAAGAGA 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 TCAATATAAAACCCAATGATCTCACC--CTCACTCCGTTTCTGATTTCTCACTCTTCGTT 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 TCTTTTTATATCTGGTTGATCAAAAAAGTAGATGATGTTATTGAATTTTCAGTGATGGAG 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ---------------------------------ATGGCGTTTCCC--TACATGGAAGCCG 
               
               
                 BASF-Gm 
                 TCTCGTTCGGTTCATCAGCGTGTGTCTCAGC-CATGGCGTTTCCC--TACATGGAAGCCG 
               
               
                 AT4g01320 
                 ---------------------------------ATGGCGATTCCT--TTCATGGAAACCG 
               
               
                 AF007269 
                 TATCTGTTGTTGTGGCATTTAGAGTAGATTCGTATTTCATCTTCTGTTTTATTCTTTTTC 
               
               
                 PPI-AtCPP 
                 ---------------------------------ATGGCGATTCCT--TTCATGGAAACCG 
               
               
                 BASF_AT2 
                 ---------------------------------ATGGCGATTCCT--TTCATGGAAACCG 
               
               
                 afc1 
                 ---------------------------------ATGGCGATTCCT--TTCATGGAAACCG 
               
               
                 BASF_AT1 
                 ---------------------------------ATGGCGATTCCT--TTCATGGAAACCG 
               
               
                 PPI-BnCPP 
                 ---------------------------------ATGGCGATTCCT--TTCATGGAAACCG 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 TTGTCGGATTTATGATATTAATGTACATTTTTGAAACTTACTTGGATGTGCGACAACATA 
               
               
                 BASF-Gm 
                 TTGTCGGATTTATGATATTAATGTACATTTTTGAAACTTACTTGGATGTGCGACAACATA 
               
               
                 AT4g01320 
                 TCGTGGGTTTTATGATAGTGATGTACATTTTTGAGACGTATTTGGATCTGAGGCAACTCA 
               
               
                 AF007269 
                 TTACAGGTTTTATGATAGTGATGTACATTTTTGAGACGTATTTGGATCTGAGGCAACTCA 
               
               
                 PPI-AtCPP 
                 TCGTGGGTTTTATGATAGTGATGTACATTTTTGAGACGTATTTGGATCTGAGGCAACTCA 
               
               
                 BASF_AT2 
                 TCGTGGGTTTTATGATAGTGATGTACATTTTTGAGACGTATTTGGATCTGAGGCAACTCA 
               
               
                 afc1 
                 TCGTGGGTTTTATGATAGTGATGTACATTTTTGAGACGTATTTGGATCTGAGGCAACTCA 
               
               
                 BASF_AT1 
                 TCGTGGGTTTTATGATAGTGATGTACATTTTTGAGACGTATTTGGATCTGAGGCAACTCA 
               
               
                 PPI-BnCPP 
                 TCGTTGGTTTTATGATAGTGATGTACGTTTTTGAGACGTATTTGGATCTGAGGCAACATA 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 GGGCCCTCAAACTTCCTACTCTTCCAAAGACTTTAGAGGGTGTTATCAGCCAAGAGAAAT 
               
               
                 BASF-Gm 
                 GGGCCCTCAAACTTCCTACTCTTCCAAAGACTTTAGAAGGTGTTATCAGCCAAGAGAAAT 
               
               
                 AT4g01320 
                 CTGCTCTCAAGCTTCCAACTCTCCCGAAAACCTTGGTTGGTGTAATTAGCCAAGAGAAGT 
               
               
                 AF007269 
                 CTGCTCTCAAGCTTCCAACTCTCCCGAAAACCTTGGTTGGTGTAATTAGCCAAGAGAAGT 
               
               
                 PPI-AtCPP 
                 CTGCTCTCAAGCTTCCAACTCTCCCGAAAACCTTGGTTGGTGTAATTAGCCAAGAGAAGT 
               
               
                 BASF_AT2 
                 CTGCTCTCAAGCTTCCAACTCTCCCGAAAACCTTGGTTGGTGTAATTAGCCAAGAGAAGT 
               
               
                 afc1 
                 CTGCTCTCAAGCTTCCAACTCTCCCGAAAACCTTGGTTGGTGTAATTAGCCAAGAGAAGT 
               
               
                 BASF_AT1 
                 CTGCTCTCAAGCTTCCAACTCTCCCGAAAACCTTGGTTGGTGTAATTAGCCAAGAGAAGT 
               
               
                 PPI-BnCPP 
                 CTGCTCTCAAGCTTCCCACTCTCCCAAAGACTTTGGTTGGAGTCATTAGCCAAGAGAAGT 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 TTGAGAAATCTAGAGCCTATAG-------------------------------------- 
               
               
                 BASF-Gm 
                 TTGAGAAATCTAGAGCCTATAG-------------------------------------- 
               
               
                 AT4g01320 
                 TTGAGAAATCACGAGCATACAG-------------------------------------- 
               
               
                 AF007269 
                 TTGAGAAATCACGAGCATACAGTCTTGACAAAAGGTTTCGTCTTGATCATATTTATATCA 
               
               
                 PPI-AtCPP 
                 TTGAGAAATCACGAGCATACAG-------------------------------------- 
               
               
                 BASF_AT2 
                 TTGAGAAATCACGAGCATACAG-------------------------------------- 
               
               
                 afc1 
                 TTGAGAAATCACGAGCATACAG-------------------------------------- 
               
               
                 BASF_AT1 
                 TTGAGAAATCACGAGCATACAG-------------------------------------- 
               
               
                 PPI-BnCPP 
                 TTGAGAAATCTCGAGCTTACAG-------------------------------------- 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------TCTTGATAAA---AGCCA 
               
               
                 BASF-Gm 
                 ------------------------------------------TCTTGATAAA---AGCCA 
               
               
                 AT4g01320 
                 ------------------------GGATATCATCACTGAGAACTTTAATATATGCAGCTA 
               
               
                 AF007269 
                 TTTTAGTTTTTTATAATTGCCAGGGGATATCATCACTGAGAACTTTAATATATGCAGCTA 
               
               
                 PPI-AtCPP 
                 ------------------------------------------TCTTGACAAA---AGCTA 
               
               
                 BASF_AT2 
                 ------------------------------------------TCTTGACAAA---AGCTA 
               
               
                 afc1 
                 ------------------------------------------TCTTGACAAA---AGCTA 
               
               
                 BASF_AT1 
                 ------------------------------------------TCTTGACAAA---AGCTA 
               
               
                 PPI-BnCPP 
                 ------------------------------------------TCTTGACAAA---AGCCA 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 CTTCCATTTTGTTCACGAGTTTGTGACAATAGTGACAGACTCTACAATTTTGTACTTTGG 
               
               
                 BASF-Gm 
                 CTTCCATTTTGTTCACGAGTTTGTGACAATAGTGACAGACTCTACAATTTTGTACTTTGG 
               
               
                 AT4g01320 
                 TTTTCACTTTGTTCATGAGTTTGTAACTATACTTATGGACTCTGCAATTTTGTTCTTTGG 
               
               
                 AF007269 
                 TTTTCACTTTGTTCATGAGTTTGTAACTATACTTATGGACTCTGCAATTTTGTTCTTTGG 
               
               
                 PPI-AtCPP 
                 TTTTCACTTTGTTCATGAGTTTGTAACTATACTTATGGACTCTGCAATTTTGTTCTTTGG 
               
               
                 BASF_AT2 
                 TTTTCACTTTGTTCATGAGTTTGTAACTATACTTATGGACTCTGCAATTTTGTTCTTTGG 
               
               
                 afc1 
                 TTTTCACTTTGTTCATGAGTTTGTAACTATACTTATGGACTCTGCAATTTTGTTCTTTGG 
               
               
                 BASF_AT1 
                 TTTTCACTTTGTTCATGAGTTTGTAACTATACTTATGGACTCTGCAATTTTGTTCTTTGG 
               
               
                 PPI-BnCPP 
                 TTTTCACTTTGTTCATGAGTTTGTTACTATACTTATGGACTCTGCGATTCTGTTCTTTGG 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 GGTATTGCCCTGGTTTTGGAAG-------------------------------------- 
               
               
                 BASF-Gm 
                 GGTATTGCCCTGGTTTTGGAAG-------------------------------------- 
               
               
                 AT4g01320 
                 GATCTTGCCTTGGTTTTGGAAG-------------------------------------- 
               
               
                 AF007269 
                 GATCTTGCCTTGGTTTTGGAAGGTACATATCTGGTTTCGGTATACAGTATCTCATTTTGA 
               
               
                 PPI-AtCPP 
                 GATCTTGCCTTGGTTTTGGAAG-------------------------------------- 
               
               
                 BASF_AT2 
                 GATCTTGCCTTGGTTTTGGAAG-------------------------------------- 
               
               
                 afc1 
                 GATCTTGCCTTGGTTTTGGAAG-------------------------------------- 
               
               
                 BASF_AT1 
                 GATCTTGCCTTGGTTTTGGAAG-------------------------------------- 
               
               
                 PPI-BnCPP 
                 GATCTTGCCTTGGTTTTGGAAG-------------------------------------- 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------AAATCAGGAGAT 
               
               
                 BASF-Gm 
                 ------------------------------------------------AAATCAGGAGAT 
               
               
                 AT4g01320 
                 ------------------------------------------------ATGTCTGGAGCT 
               
               
                 AF007269 
                 ATATAGAGTTGTTACATTACAATTGTAAAGTTTTCATTTTTACCTTAGATGTCTGGAGCT 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------ATGTCTGGAGCT 
               
               
                 BASF_AT2 
                 ------------------------------------------------ATGTCTGGAGCA 
               
               
                 afc1 
                 ------------------------------------------------ATGTCTGGAGCT 
               
               
                 BASF_AT1 
                 ------------------------------------------------ATGTCTGGAGCT 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------ATATCTGGCGGC 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 TTTATGACAATAGCTGGTTTCAATGCTGAGAATGAAATACTGCATACCCTTGCCTTCTTA 
               
               
                 BASF-Gm 
                 TTTATGACAATAGCTGGTTTCAATGCTGAGAATGAAATACTGCATACCCTTGCCTTCTTA 
               
               
                 AT4g01320 
                 GTTTTACCGAGGTTGGGCCTTGATCCAGAGAATGAAATACTGCATACTCTTTCATTCTTG 
               
               
                 AF007269 
                 GTTTTACCGAGGTTGGGCCTTGATCCAGAGAATGAAATACTGCATACTCTTTCATTCTTG 
               
               
                 PPI-AtCPP 
                 GTTTTACCGAGGTTGGGCCTTGATCCGGAGAATGAAATACTGCATACTCTTTCATTCTTG 
               
               
                 BASF_AT2 
                 GTTTTACCGAGGTTGGGCCTTGATCCAGAGAATGAAATACTGCATACTCTTTCATTCTTG 
               
               
                 afc1 
                 GTTTTACCGAGGTTGGGCCTTGATCCAGAGAATGAAATACTGCATACTCTTTCATTCTTG 
               
               
                 BASF_AT1 
                 GTTTTACCGAGGTTGGGCCTTGATCCAGAGAATGAAATACTGCATACTCTTTCATTCTTG 
               
               
                 PPI-BnCPP 
                 TTTCTACCAATGGTGGGACTCGATCCAGAGAATGAAATCCTGCACACTCTTTCATTCTTG 
               
               
                 BASF-Corn 
                 ------------ACGAGGCTGAGTGCTGAGAATGAGATAATACACACCCTTGCTTTCTTA 
               
               
                   
                                 *  *   * * ******** **  * ** ** *** * ***** 
               
               
                   
               
               
                 PPI-GmCPP 
                 GCAGGGCTGATGATTTGGTCACAG------------------------------------ 
               
               
                 BASF-Gm 
                 GCAGGGCTGATGATTTGGTCACAG------------------------------------ 
               
               
                 AT4g01320 
                 GCTGGTGTTATGACATGGTCACAG------------------------------------ 
               
               
                 AF007269 
                 GCTGGTGTTATGACATGGTCACAGGTGTTCCAAATAAACCCCTTCATATAGTCCTATACG 
               
               
                 PPI-AtCPP 
                 GCTGGTGTTATGACATGGTCACAG------------------------------------ 
               
               
                 BASF_AT2 
                 GCTGGTGTTATGACATGGTCACAG------------------------------------ 
               
               
                 afc1 
                 GCTGGTGTTATGACATGGTCACAG------------------------------------ 
               
               
                 BASF_AT1 
                 GCTGGTGTTATGACATGGTCACAC------------------------------------ 
               
               
                 PPI-BnCPP 
                 GCTGGTCTTATGACATGGTCACAG------------------------------------ 
               
               
                 BASF-Corn 
                 GCTGGTTCCATGGTTTGGTCGCAG------------------------------------ 
               
               
                   
                 ** **    ***   ***** ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 TTTAGCATCAPAATATCTATTTTCTTAAGATAATAATATTTCTTTTATATTCTGATGCAG 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ATAACAGATTTGCCCTTTTCTCTGTACTCAACTTTTGTGATTGAGGCCCGTCATGGTTTT 
               
               
                 BASF-Gm 
                 ATAACAGATTTGCCCTTTTCTCTGTACTCAACTTTTGTGATTGAGGCCCGTCATGGTTTT 
               
               
                 AT4g01320 
                 ATCACTGATTTGCCATTTTCTTTGTACTCAACTTTCGTGATCGAGTCTCGGCATGGGTTC 
               
               
                 AF007269 
                 ATCACTGATTTGCCATTTTCTTTGTACTCAACTTTCGTGATCGAGTCTCGGCATGGGTTC 
               
               
                 PPI-AtCPP 
                 ATCACTGATTTGCCATTTTCTTTGTACTCAACTTTCGTGATCGAGTCTCGGCATGGGTTC 
               
               
                 BASF_AT2 
                 ATCACTGATTTGCCATTTTCTTTGTACTCAACTTTCGTGATCGAGTCTCGGCATGGGTTC 
               
               
                 afc1 
                 ATCACTGATTTGCCATTTTCTTTGTACTCAACTTTCGTGATCGAGTCTCGGCATGGGTTC 
               
               
                 BASF_AT1 
                 ATCACTGATTTGCCATTTTCTTTGTACTCAACTTTCGTGATCGAGTCTCGGCATGGGTTC 
               
               
                 PPI-BnCPP 
                 ATCACTGATTTGCCATTTTCTTTGTACTCAACTTTCGTGATCGAGTCTCGGCATGGGTTC 
               
               
                 BASF-Corn 
                 ATTACAGACTTGCCGTTCTCTCTCTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTT 
               
               
                   
                 ** ** ** ***** ** *** * ** ******** ** **  *** * ** ***** ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 AATAAG------------------------------------------------------ 
               
               
                 BASF-Gm 
                 AATAAG------------------------------------------------------ 
               
               
                 AT4g01320 
                 AACAAA------------------------------------------------------ 
               
               
                 AF007269 
                 AACAAAGTATGTCGTATTTCCAACACTACCTTGTGACTTACGTTTTTTTATCAGAGATGT 
               
               
                 PPI-AtCPP 
                 AACAAA------------------------------------------------------ 
               
               
                 BASF_AT2 
                 AACAAA------------------------------------------------------ 
               
               
                 afc1 
                 AACAAA------------------------------------------------------ 
               
               
                 BASF_AT1 
                 AACAAA------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 AACAAA------------------------------------------------------ 
               
               
                 BASF-Corn 
                 AACAAG------------------------------------------------------ 
               
               
                   
                 ** ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 --------------------------------CAAACACCATGGTTATTCTTTAGGGACA 
               
               
                 BASF-Gm 
                 --------------------------------CAAACACCATGGTTATTCTTTAGGGACA 
               
               
                 AT4g01320 
                 --------------------------------CAAACAATATGGATGTTCATTAGGGACA 
               
               
                 AF007269 
                 GGATTAATTTGCTTCTAAATTCTGTTGACAGCAAACAATATGGATGTTCATTAGGGACA 
               
               
                 PPI-AtCPP 
                 --------------------------------CAAACAATATGGATGTTCATTAGGGACA 
               
               
                 BASF_AT2 
                 --------------------------------CAAACAATATGGATGTTCATTAGGGACA 
               
               
                 afc1 
                 --------------------------------CAAACAATATGGATGTTCATTAGGGACA 
               
               
                 BASF_AT1 
                 --------------------------------CAAACAATATGGATGTTCATTAGGGACA 
               
               
                 PPI-BnCPP 
                 --------------------------------CAAACAATATGGATGTTCATTAGGGACA 
               
               
                 BASF-Corn 
                 --------------------------------CAAACTATATGGCTCTTCATTAGGGATA 
               
               
                   
                                                 *****   **** * *** ******* * 
               
               
                   
               
               
                 PPI-GmCPP 
                 TGCTTAAAGGAATTTTCCTTTCTGTAATAATTGGTCCACCTATTGTGGCTGCAATCATTG 
               
               
                 BASF-Gm 
                 TGCTTAAAGGAATTTTCCTTTCCGTAATAATTGGTCCACCTATTGTGGCTGCAATCATTG 
               
               
                 AT4g01320 
                 TGATCAAAGGAACATTCCTCTCTGTCATACTAGGCCCACCCATTGTTGCTGCGATAATTT 
               
               
                 AF007269 
                 TGATCAAAGGAACATTCCTCTCTGTCATACTAGGCCCACCCATTGTTGCTGCGATAATTT 
               
               
                 PPI-AtCPP 
                 TGATCAAAGGAACATTCCTCTCTGTCATACTAGGCCCACCCATTGTTGCTGCGATAATTT 
               
               
                 BASF_AT2 
                 TGATCAAAGGAACATTCCTCTCTGTCATACTAGGCCCACCCATTGTTGCTGCGATAATTT 
               
               
                 afc1 
                 TGATCAAAGGAACATTCCTCTCTGTCATACTAGGCCCACCCATTGTTGCTGCGATAATTT 
               
               
                 BASF_AT1 
                 TGATCAAAGGAACATTCCTCTCTGTCATACTAGGCCCACCCATTGTTGCCGCGATAATTT 
               
               
                 PPI-BnCPP 
                 TGATCAAAGGAATACTCCTCTCTGTCATACCTGCCCCTCCTATCGTTGCCGCAATTATTG 
               
               
                 BASF-Corn 
                 TGATCAAAGGAATTTTACTATCCATGATATTGGGGCCACCAATCGTGGCTGCTATCATCT 
               
               
                   
                 ** * *******   * ** **  * ***   *  ** ** ** ** ** ** ** ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 TAATAGTACAG------------------------------------------------- 
               
               
                 BASF-Gm 
                 TAATAGTACAG------------------------------------------------- 
               
               
                 AT4g01320 
                 TCATAGTCCAG------------------------------------------------- 
               
               
                 AF007269 
                 TCATAGTCCAGGTTTGATGATTCTGGATTCATCTTATTTCTGAGTTTTTCACATGGATGA 
               
               
                 PPI-AtCPP 
                 TCATAGTCCAG------------------------------------------------- 
               
               
                 BASF_AT2 
                 TCATAGTCCAG------------------------------------------------- 
               
               
                 afc1 
                 TCATAGTCCAG------------------------------------------------- 
               
               
                 BASF_AT1 
                 TCATAGTCCAG------------------------------------------------- 
               
               
                 PPI-BnCPP 
                 TTATAGTTCAG------------------------------------------------- 
               
               
                 BASF-Corn 
                 ACATAGTACAG------------------------------------------------- 
               
               
                   
                   ***** *** 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 CTATTCTCCATTGAGTGTGAGCTTCAAAGTTTTTAGTTTTCGTGTTAAAAATTTAAAATT 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------AAAGGAGGTCCATACTTGGCCATC 
               
               
                 BASF-Gm 
                 ------------------------------------AAAGGAGGTCCATACTTGGCCATC 
               
               
                 AT4g01320 
                 ------------------------------------AAAGGAGGTCCTTATCTTGCCATC 
               
               
                 AF007269 
                 TGCTTCTCTGAGCATGAAGTTTCTATCTTTTTCCAGAAAGGAGGTCCTTATCTTGCCATC 
               
               
                 PPI-AtCPP 
                 ------------------------------------AAAGGAGGTCCTTATCTTGCCATC 
               
               
                 BASF_AT2 
                 ------------------------------------AAAGGAGGTCCTTATCTTGCCATC 
               
               
                 afc1 
                 ------------------------------------AAAGGAGGTCCTTATCTTGCCATC 
               
               
                 BASF_AT1 
                 ------------------------------------AAAGGAGGTCCTTATCTTGCCATC 
               
               
                 PPI-BnCPP 
                 ------------------------------------AAAGGAGGTCCTTACCTCGCCATC 
               
               
                 BASF-Corn 
                 ------------------------------------ATTGGAGGACCTTACCTGGCTATA 
               
               
                   
                                                     *  ***** ** **  * ** ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 TATCTTTGGGTTTTTACGTTTGGTCTTTCTATTGTGATGATGACCCTTTATCCAGTACTA 
               
               
                 BASF-Gm 
                 TATCTTTGGGTTTTTACGTTTGGTCTTTCTATTGTGATGATGACCCTTTATCCAGTACTA 
               
               
                 AT4g01320 
                 TATCTGTGGGCATTCATGTTTATCCTGTCTCTAGTGATGATGACTATATACCCGGTCTTG 
               
               
                 AF007269 
                 TATCTGTGGGCATTCATGTTTATCCTGTCTCTAGTGATGATGACTATATACCCGGTCTTG 
               
               
                 PPI-AtCPP 
                 TATCTGTGGGCATTCATGTTTATCCTGTCTCTAGTGATGATGACTATATACCCGGTCTTG 
               
               
                 BASF_AT2 
                 TATCTGTGGGCATTCATGTTTATCCTGTCTCTAGTGATGATGACTATATACCCGGTCTTG 
               
               
                 afc1 
                 TATCTGTGGGCATTCATGTTTATCCTGTCTCTAGTGATGATGACTATATACCCGGTCTTG 
               
               
                 BASF_AT1 
                 TATCTGTGGGCATTCATGTTTATCCTGTCTCTAGTGATGATGACTATATACCCGGTCTTG 
               
               
                 PPI-BnCPP 
                 TATCTGTGGGCATTCATGTTTATCCTGTCTCTAGTGATGATGACTATATACCCTGTTTTG 
               
               
                 BASF-Corn 
                 TATCTCTGGGGTTTTATGTTTGTATTAGCTCTACTGATGATGACAATATACCCCATTGTG 
               
               
                   
                 ***** ****  ** * ****    *  ** *   **********  * ** **  *  * 
               
               
                   
               
               
                 PPI-GmCPP 
                 ATAGCTCCACTCTTCAATAAGTTCACTCCA------------------------------ 
               
               
                 BASF-Gm 
                 ATAGCTCCACTCTTCAATAAGTTCACTCCA------------------------------ 
               
               
                 AT4g01320 
                 ATAGCACCGCTCTTCAACAAGTTCACTCCT------------------------------ 
               
               
                 AF007269 
                 ATAGCACCGCTCTTCAACAAGTTCACTCCTGTGTGTATTTCTGTCATGGCCATTTTACAA 
               
               
                 PPI-AtCPP 
                 ATAGCACCGCTCTTCAACAAATTCACTCCT------------------------------ 
               
               
                 BASF_AT2 
                 ATAGCACCGCTCTTCAACAAGTTCACTCCT------------------------------ 
               
               
                 afc1 
                 ATAGCACCGCTCTTCAACAAGTTCACTCCT------------------------------ 
               
               
                 BASF_AT1 
                 ATAGCACCGCTCTTCAACAAGTTCACTCCT------------------------------ 
               
               
                 PPI-BnCPP 
                 ATTGCACCTCTTTTCAACAAGTTCACTCCT------------------------------ 
               
               
                 BASF-Corn 
                 ATAGCTCCTCTGTTCAACAAGTTCACTCCT------------------------------ 
               
               
                   
                 ** ** ** ** ***** ** ******** 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 TTCACTGCTTGTTTGCATATGTTGTTACCAGACAATATAATCTCCCGCTTTTTTATGGCT 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ----CTTCCAGATGGTCAACTCAGGGAGAAAATCGAGAAACTTGCTTCCTCCCTCAACTA 
               
               
                 BASF-Gm 
                 ----CTTCCAGATGGTCAACTCAGGGAGAAAATCGAGAAACTTGCTTCCTCCCTCAACTA 
               
               
                 AT4g01320 
                 ----CTTCCAGATGGAGACCTCCGGGAGAAGATTGAGAAACTTGCTTCTTCTCTAAAGTT 
               
               
                 AF007269 
                 ATAGCTTCCAGATGGAGACCTCCGGGAGAAGATTGAGAAACTTGCTTCTTCTCTAAAGTT 
               
               
                 PPI-AtCPP 
                 ----CTTCCAGATGGAGACCTCCGGGAGAAGATTGAGAAACTTGCTTCTTCCCTAAAGTT 
               
               
                 BASF_AT2 
                 ----CTTCCAGATGGAGACCTCCGGGAGAAGATTGAGAAACTTGCTTCTTCTCTAAAGTT 
               
               
                 afc1 
                 ----CTTCCAGATGGAGACCTCCGGGAGAAGATTGAGAAACTTGCTTCTTCTCTAAAGTT 
               
               
                 BASF_AT1 
                 ----CTTCCAGATGGAGACCTCCGGGAGAAGATTGAGAAACTTGCTTCTTCTCTAAAGTT 
               
               
                 PPI-BnCPP 
                 ----CTTCCTGATGGAGACCTCCGGGAGAAGATTGAGAAACTTGCTTCTTCTCTAAAGTT 
               
               
                 BASF-Corn 
                 ----CTTCCTGAAGGAGTCCTCAGGGAAAAAATAGAGAAGCTGGCAGCTTCCCTCAAGTT 
               
               
                   
                     ***** ** **    *** ****  ** **  ***** ** **  * ** ** ** * 
               
               
                   
               
               
                 PPI-GmCPP 
                 TCCGTTAAAGAAACTATTTGTTGTCGATGGATCCACAAGATCAAGTCACAGCAATG---- 
               
               
                 BASF-Gm 
                 TCCGTTAAAGAAACTATTTGTTGTCGATGGATCCACAAGATCAAGTCACAGCAATG---- 
               
               
                 AT4g01320 
                 TCCTTTGAAGAAGCTGTTTGTTGTCGATGGATCTACAAGGTCAAGCCATAGCAATG---- 
               
               
                 AF007269 
                 TCCTTTGAAGAAGCTGTTTGTTGTCGATGGATCTACAAGGTCAAGCCATAGCAATGTGAG 
               
               
                 PPI-AtCPP 
                 TCCTTTGAAGAAGCTGTTTGTTGTCGATGGATCTACAAGGTCAAGCCATAGCAATG---- 
               
               
                 BASF_AT2 
                 TCCTTTGAAGAAGCTGTTTGTTGTCGATGGATCTACAAGGTCAAGCCATAGCAATG--- 
               
               
                 afc1 
                 TCCTTTGAAGAAGCTGTTTGTTGTCGATGGATCTACAAGGTCAAGCCATAGCAATG---- 
               
               
                 BASF_AT1 
                 TCCTTTGAAGAAGCTGTTTGTTGTCGATGGATCTACAAGGTCAAGCCATAGCAATG--- 
               
               
                 PPI-BnCPP 
                 TCCTCTGAAGAAGCTGTTTGTTGTCGATGGATCTACAAGGTCAAGCCATAGTAATG---- 
               
               
                 BASF-Corn 
                 TCCTTTGAAAAAGCTTTTCGTGGTAGATGGGTCTACCAGATCAAGCCACAGTAATG--- 
               
               
                   
                 ***  * ** ** ** ** ** ** ***** ** ** ** ***** ** ** **** 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 AAGCTTGAGATCTCTTCCTACCTACTTTACTCTAGTTTACCATTAGAAGCTTACGTATCT 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ----------------CCTATATGTATGGATTCTTCAAGAACAAGAGGATTGTCCCTTA 
               
               
                 BASF-Gm 
                 ----------------CCTATATGTATGGATTCTTCAAGAACAAGAGGATTGTCCTTTA 
               
               
                 AT4g01320 
                 ----------------CTTACATGTATGGTTTCTTTAAGAACAAAAGGATTGTTCTTTAT 
               
               
                 AF007269 
                 TGTTACATCATACAGGCTTACATGTATGGTTTCTTTAAGAACAAAAGGATTGTTCTTTAP 
               
               
                 PPI-AtCPP 
                 ----------------CTTACATGTATGGTTTCTTTAAGAACAAAAGGATTGTTCTTTAT 
               
               
                 BASF_AT2 
                 ----------------CTTACATGTATGGTTTCTTTAAGAACAAAAGGATTGTTCTTTAT 
               
               
                 afc1 
                 ----------------CTTACATGTATGGTTTCTTTAAGAACAAAAGGATTGTTCTTTAT 
               
               
                 BASF_AT1 
                 ----------------CTTACATGTATGGTTTCTTTAAGAACAAAAGGATTGTTCTTTA 
               
               
                 PPI-BnCPP 
                 ----------------CTTACATGTATGGTTTCTTCAAGAACAAAAGGATTGTTCTTTAT 
               
               
                 BASF-Corn 
                 ----------------CCTACATGTATGGTTTTTTCAAGAACAAGCGCATAGTACTCTAT 
               
               
                   
                                 * ** ******** ** ** ********  * ** ** *  *** 
               
               
                   
               
               
                 PPI-GmCPP 
                 GACACATTAATTCAACAG------------------------------------------ 
               
               
                 BASF-Gm 
                 GACACATTAATTCAACAG------------------------------------------ 
               
               
                 AT4g01320 
                 GATACGTTGATTCAGCAG------------------------------------------ 
               
               
                 AF007269 
                 GATACGTTGATTCAGCAGGTACTGTGACTCTTGATGCTTCAAACGAGCTATACTCACATT 
               
               
                 PPI-AtCPP 
                 GATACGTTGATTCAGCAG------------------------------------------ 
               
               
                 BASF_AT2 
                 GATACGTTGATTCAGCAG------------------------------------------ 
               
               
                 afc1 
                 GATACGTTGATTCAGCAG------------------------------------------ 
               
               
                 BASF_AT1 
                 GATACGTTGATTCAGCAG------------------------------------------ 
               
               
                 PPI-BnCPP 
                 GACACATTGATTCAGCAG------------------------------------------ 
               
               
                 BASF-Corn 
                 GACACATTGATTCAGCAG------------------------------------------ 
               
               
                   
                 ** ** ** ***** *** 
               
               
                   
               
               
                 PPI-GmCPP 
                 --------------------------------------------TGCAAAGACGATGAGG 
               
               
                 BASF-Gm 
                 --------------------------------------------TGCAAAGACGATGAGG 
               
               
                 AT4g01320 
                 --------------------------------------------TGCAAGAATGAGGATG 
               
               
                 AF007269 
                 TCTGTTTCTGGTTCTGAAACATAACATAATCTTCTATTGTGCAGTGCAAGAATGAGGATG 
               
               
                 PPI-AtCPP 
                 --------------------------------------------TGCAAGAATGAGGATG 
               
               
                 BASF_AT2 
                 --------------------------------------------TGCAAGAATGAGGATG 
               
               
                 afc1 
                 --------------------------------------------TGCAAGAATGAGGATG 
               
               
                 BASF_AT1 
                 --------------------------------------------TGCAAGAATGAGGATG 
               
               
                 PPI-BnCPP 
                 --------------------------------------------TGCCAGAATGAGAATG 
               
               
                 BASF-Corn 
                 --------------------------------------------TGTAGCAATGAGGATG 
               
               
                   
                                                             **     * **  * * 
               
               
                   
               
               
                 PPI-GmCPP 
                 AAATTGTTGCTGTTATTGCCCATGAGTTGGGACACTGGAAGCTCAACCATACTGTGTACA 
               
               
                 BASF-Gm 
                 AAATTGTTGCTGTTATTGCCCATGAGTTGGGACACTGGAAGCTCAACCATACTGTGTACA 
               
               
                 AT4g01320 
                 AAATTGTGGCGGTTATTGCACACGAGCTTGGACATTGGAAACTGAATCACACTACATACT 
               
               
                 AF007269 
                 AAATTGTGGCGGTTATTGCACACGAGCTTGGACATTGGAAACTGAATCACACTACATACT 
               
               
                 PPI-AtCPP 
                 AAATTGTGGCGGTTATTGCACACGAGCTTGGACATTGGAAACTGAATCACACTACATACT 
               
               
                 BASF_AT2 
                 AAATTGTGGCGGTTATTGCACACGAGCTTGGACATTGGAAACTGAATCACACTACATACT 
               
               
                 afc1 
                 AAATTGTGGCGGTTATTGCACACGAGCTTGGACATTGGAAACTGAATCACACTACATACT 
               
               
                 BASF_AT1 
                 AAATTGTGGCGGTTATTGCACACGAGCTTGGACATTGGAAACTGAATCACACTACATACT 
               
               
                 PPI-BnCPP 
                 AAATTGTGGCGGTTATTGCACACGAGCTGGGACACTGGAAGCTGAATCACACTACATACT 
               
               
                 BASF-Corn 
                 AGATAGTTTCTGTTATAGCACATGAACTTGGACACTGGAAACTCAATCATACTGTCTATT 
               
               
                   
                 * ** **  * ***** ** ** **  * ***** ***** ** ** ** ***   ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 CATTTGTTGCTATGCAG------------------------------------------- 
               
               
                 BASF-Gm 
                 CATTTGTTGCTATGCAG------------------------------------------- 
               
               
                 AT4g01320 
                 CGTTCATTGCAGTTCAA------------------------------------------- 
               
               
                 AF007269 
                 CGTTCATTGCAGTTCAAGTGAGGCTCAACCGACAGTTCAAAAACTTACTCACATCTACAT 
               
               
                 PPI-AtCPP 
                 CGTTCATTGCAGTTCAA------------------------------------------- 
               
               
                 BASF_AT2 
                 CGTTCATTGCAGTTCAA------------------------------------------- 
               
               
                 afc1 
                 CGTTCATTGCAGTTCAA------------------------------------------- 
               
               
                 BASF_AT1 
                 CGTTCATTGCAGTTCAA------------------------------------------- 
               
               
                 PPI-BnCPP 
                 CGTTCATTGCTGTTCAA------------------------------------------- 
               
               
                 BASF-Corn 
                 CCTTTGTAGCTGTCCAG------------------------------------------- 
               
               
                   
                 * **  * **  * ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 ---------------------------------------------------ATTCTTACA 
               
               
                 BASF-Gm 
                 ---------------------------------------------------ATTCTTACA 
               
               
                 AT4g01320 
                 ---------------------------------------------------ATCCTTGCC 
               
               
                 AF007269 
                 TTCACTTAAGAAATCATGTCTTATGACCCTCTCTCAATGTTTTGCTTGCAGATCCTTGCC 
               
               
                 PPI-AtCPP 
                 ---------------------------------------------------ATCCTTGCC 
               
               
                 BASF_AT2 
                 ---------------------------------------------------ATCCTTGCC 
               
               
                 afc1 
                 ---------------------------------------------------ATCCTTGCC 
               
               
                 BASF_AT1 
                 ---------------------------------------------------ATCCTTGCC 
               
               
                 PPI-BnCPP 
                 ---------------------------------------------------ATCCTTGCC 
               
               
                 BASF-Corn 
                 ---------------------------------------------------CTGCTTATG 
               
               
                   
                                                                     * *** 
               
               
                   
               
               
                 PPI-GmCPP 
                 CTTCTACAATTTGGAGGATATACACTAGTGCGAAATTCAGCTGATCTGTATCGAAGCTTT 
               
               
                 BASF-Gm 
                 CTTCTACAATTTGGAGGATATACACTAGTGCGAAATTCAGCTGATCTGTATCGAAGCTTT 
               
               
                 AT4g01320 
                 TTCTTACAATTTGGAGGATACACTCTTGTCAGAAACTCCACTGATCTCTTCAGGAGTTTC 
               
               
                 AF007269 
                 TTCTTACAATTTGGAGGATACACTCTTGTCAGAAACTCCACTGATCTCTTCAGGAGTTTC 
               
               
                 PPI-AtCPP 
                 TTCTTACAATTTGGAGGATACACTCTTCTCAGAAACTCCACTGATCTCTTCAGGAGTTTC 
               
               
                 BASF_AT2 
                 TTCTTACAATTTGGAGGATACACTCTTGTCAGAAACTCCACTGATCTCTTCAGGAGTTTC 
               
               
                 afc1 
                 TTCTTACAATTTGGAGGATACACTCTTGTCAGAAACTCCACTGATCTCTTCAGGAGTTTC 
               
               
                 BASF_AT1 
                 TTCTTACAATTTGGAGGATACACTCTTGTCAGAAACTCCACTGATCTCTTCAGGAGTTTC 
               
               
                 PPI-BnCPP 
                 TTCTTGCAATTTGGAGGATACACTCTTGTCAGAAACTCCACTGATCTCTTCAGGAGTTTT 
               
               
                 BASF-Corn 
                 TTTCTTCAATTTGGAGGATATACTCTAGTAAGGAGCTCCAAAGATCTATTTGGAAGTTTT 
               
               
                   
                  *  * ************** ** **  *  * *  **    ***** *   *  ** ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 GGGTTTGATACGCAGCCAGTCCTCATTGGGCTCATCATATTTCAG--------------- 
               
               
                 BASF-Gm 
                 GGGTTTGATACGCAGCCAGTCCTCATTGGGCTCATCATATTTCAG--------------- 
               
               
                 AT4g01320 
                 GGATTTGATACACAGCCTGTTCTCATTGGTTTGATCATATTTCAG--------------- 
               
               
                 AF007269 
                 GGATTTGATACACAGCCTGTTCTCATTGGTTTGATCATATTTCAGGTTTGTTATTTTTGC 
               
               
                 PPI-AtCPP 
                 GGATTTGATACACAGCCTGTTCTCATTGGTTTGATCATATTTCAG--------------- 
               
               
                 BASF_AT2 
                 GGATTTGATACACAGCCTGTTCTCATTGGTTTGATCATATTTCAG--------------- 
               
               
                 afc1 
                 GGATTTGATACACAGCCTGTTCTCATTGGTTTGATCATATTTCAG--------------- 
               
               
                 BASF_AT1 
                 GGATTTGATACACAGCCTGTTCTCATTGGTTTGATCATATTTCAG--------------- 
               
               
                 PPI-BnCPP 
                 GGTTTTGATACACAACCAGTTCTCATTGGTTTGATCATATTTCAG--------------- 
               
               
                 BASF-Corn 
                 GGCTTCAAGGACCAGCCAGTAATAATTGGATTGATCATTTTCCCG--------------- 
               
               
                   
                 ** **  *    ** ** **  * *****  * ***** ** * * 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 CTTTTGACACTAATCTAATGAATCAAGGATGGATTAAGAAAAAAAAACTCTAAACCTTTG 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ---------------------------CATACTGTAATCCCACTTCAGCAATTGGTCAGC 
               
               
                 BASF-Gm 
                 ---------------------------CATACTGTAATCCCACTTCAGCAATTGGTCAGC 
               
               
                 AT4g01320 
                 ---------------------------CACACTGTAATACCACTGCAACATCTAGTAAGC 
               
               
                 AF007269 
                 GTTATATCTCCTGTCTGATTATCACAGCACACTGTAATACCACTGCAACATCTAGTAAGC 
               
               
                 PPI-AtCPP 
                 ---------------------------CACACTGTAATACCACTGCAACATCTAGTAAGC 
               
               
                 BASF_AT2 
                 ---------------------------CACACTGTAATACCACTGCAACATCTAGTAAGC 
               
               
                 afc1 
                 ---------------------------CACACTGTAATACCACTGCAACATCTAGTAAGC 
               
               
                 BASF_AT1 
                 ---------------------------CACACTGTAATACCACTGCAACATCTAGTAAGC 
               
               
                 PPI-BnCPP 
                 ---------------------------CACACTGTAATACCACTTCAACACCTAGTAAGC 
               
               
                 BASF-Corn 
                 ---------------------------CACACCATAATACCCATCCAACACCTTCTGAGC 
               
               
                   
                                            ** **  **** **  * ** **     * *** 
               
               
                   
               
               
                 PPI-GmCPP 
                 TTTGGTCTGAACCTAGTCAGCCGATCATTTGAATTTCAGG-------------------- 
               
               
                 BASF-Gm 
                 TTTGGTCTGAACCTAGTCAGCCGATCATTTGAATTTCAGG-------------------- 
               
               
                 AT4g01320 
                 TTTGGCCTGAACCTCGTTAGTCGAGCGTTTGAGTTTCAGG-------------------- 
               
               
                 AF007269 
                 TTTGGCCTGAACCTCGTTAGTCGAGCGTTTGAGTTTCAGGTACCATCTTACAATCCCTCA 
               
               
                 PPI-AtCPP 
                 TTTGGCCTGAACCTCGTTAGTCGAGCGTTTGAGTTTCAGG-------------------- 
               
               
                 BASF_AT2 
                 TTTGGCCTGAACCTCGTTAGTCGAGCGTTTGAGTTTCAGG-------------------- 
               
               
                 afc1 
                 TTTGGCCTGAACCTCGTTAGTCGAGCGTTTGAGTTTCAGG-------------------- 
               
               
                 BASF_AT1 
                 TTTGGCCTCAACCTTGTTAGTCGAGCGTTTGAGTTTCAGG-------------------- 
               
               
                 PPI-BnCPP 
                 TTTGACCTCAACCTTGTTAGTCGAGCGTTTGAGTTTCAGG-------------------- 
               
               
                 BASF-Corn 
                 TTTCGCCTGAACCTTGTCAGCAGAGCATTTGAATTTCAGG-------------------- 
               
               
                   
                 ***   ** ***** ** **  ** * ***** ******* 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 AGATCCAACCATAGTTTCTTTATTGCAATGGCAGCCTCATCTACTAATCTGAGTTAACGT 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------CTGATGGCTTTGCCAAGAAGCTTGGATATGCATCTGGATTACGCGGTG 
               
               
                 BASF-Gm 
                 ------------CTGATGGCTTTGCCAAGAAGCTTGGATATGCATCTGGATTACGCGGTG 
               
               
                 AT4g01320 
                 ------------CTGATGCTTTTGCTGTGAAGCTTGGCTATGCAAAAGATCTTCGTCCTG 
               
               
                 AF007269 
                 TCCTTTTGCAGGCTGATGCTTTTGCTGTGAAGCTTGGCTATGCAAAAGATCTTCGTCCTG 
               
               
                 PPI-AtCPP 
                 ------------CTGATGCTTTTGCTGTGAAGCTTGACTATGCAAAAGATCTTCGTCCTG 
               
               
                 BASF_AT2 
                 ------------CTGATGCTTTTGCTGTGAAGCTTGGCTATGCAAAAGATCTTCGTCCTG 
               
               
                 afc1 
                 ------------CTGATGCTTTTGCCGTGAAGCTTGGCTATGCAAAAGATCTTCGTCCTG 
               
               
                 BASF_AT1 
                 ------------CTGATGCTTTTGCTGTGAAGCTTGGCTATGCAAAAGATCTTCGTCCTA 
               
               
                 PPI-BnCPP 
                 ------------CTGATGCTTTTGCAGTGAATCTTGGTTATGCAAAGGATCTACGTCCTG 
               
               
                 BASF-Corn 
                 ------------CTGATGCCTTTGCCAAGAACCTTGGATATGCCCCTCAGCTCCGAGCAG 
               
               
                   
                             ******  *****   *** ****  *****        * ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 GTCTTGTGAAACTACAGG------------------------------------------ 
               
               
                 BASF-Gm 
                 GTCTTGTGAAACTACAGG------------------------------------------ 
               
               
                 AT4g01320 
                 CTCTAGTGAAACTACAGGTCAGAGAAGATAACAACAGAACACAAACTGTTACCTCAATTT 
               
               
                 AF007269 
                 CTCTAGTGAAACTACAGGTCAGAGAAGATAACAACAGAACACAAACTGTTACCTCAATTT 
               
               
                 PPI-AtCPP 
                 CTCTAGTGAAACTACAGG------------------------------------------ 
               
               
                 BASF_AT2 
                 CTCTAGTGAAACTACAGG------------------------------------------ 
               
               
                 afc1 
                 CTCTAGTGAAACTACAGG------------------------------------------ 
               
               
                 BASF_AT1 
                 CTCTAGTGAAACTACAGG------------------------------------------ 
               
               
                 PPI-BnCPP 
                 CCCTAGTGAAGCTACAGG------------------------------------------ 
               
               
                 BASF-Corn 
                 CCCTTGTTAAACTACAGG------------------------------------------ 
               
               
                   
                   ** ** **  ******* 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------AGGAGAATCTGTCAGCTA 
               
               
                 BASF-Gm 
                 ------------------------------------------AGGAGAATCTGTCAGCTA 
               
               
                 AT4g01320 
                 GTGTCACACACTTAAATGGATTTTTTGTTGGGATTTTGCAGGAAGAGAACTTATCAGCAA 
               
               
                 AF007269 
                 GTGTCACACACTTAAATGGATTTTTTGTTGGGATTTTGCAGGAAGAGAACTTATCAGCAA 
               
               
                 PPI-AtCPP 
                 ------------------------------------------AAGAGAACTTATCAACAA 
               
               
                 BASF_AT2 
                 ------------------------------------------AAGAGAACTTATCAGCAA 
               
               
                 afc1 
                 ------------------------------------------AAGAGAACTTATCAGCAA 
               
               
                 BASF_AT1 
                 ------------------------------------------AAGAGAACTTATCAGCAA 
               
               
                 PPI-BnCPP 
                 ------------------------------------------AAGAGAACTTATCAGCGA 
               
               
                 BASF-Corn 
                 ------------------------------------------AGGAGAACTTGTCTGCGA 
               
               
                   
                                                           * *****  * **  * * 
               
               
                   
               
               
                 PPI-GmCPP 
                 TGAATACAGATCCTTGGTACTCTGCTTATCACTATTCTCATCCTCCCCTTGTTGAAAGAT 
               
               
                 BASF-Gm 
                 TGAATACAGATCCTTGCT--CGTGCCG--------------------------------- 
               
               
                 AT4g01320 
                 TGAACACTGATCCATTGTACTCAGCTTATCACTACTCACATCCTCCTCTTGTTGAAAGGC 
               
               
                 AF007269 
                 TGAACACTGATCCATTGTACTCAGCTTATCACTACTCACATCCTCCTCTTGTTGAAAGGC 
               
               
                 PPI-AtCPP 
                 TGAACACTGATCCATTGTACTCAGCTTATCACTACTCACATCCTCCTCTTGTTGAAAGGC 
               
               
                 BASF_AT2 
                 TGAAAACTGATCTATTGTACTCAGCTTATCACTACTCACATCCTCCTCTTGTTGAAAGGC 
               
               
                 afc1 
                 TGAACACTGATCCATTGCACTCAGCTTATCACTACTCACATCCTCCTCTTGTTGAAAGGC 
               
               
                 BASF_AT1 
                 TGAATACTGATCCATTGTACTCAGCTTATCACTACTCACATCCTCCTCTTGTTGAAAGGC 
               
               
                 PPI-BnCPP 
                 TGAACACAGACCCATTGTACTCAGCTTATCACTACTCACACCCTCCTCTTGTAGAGAGGC 
               
               
                 BASF-Corn 
                 TGAACACCGATCCTTGGTATTCGGCATATCACTACTCCCACCCACCACTCGTCGAGAGGC 
               
               
                   
                 **** ** ** *  *        ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 TGGCCGCGCTGGACGA---ACCGGATAAGAAGGAAGACTAA------------------- 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 TTCGAGCCATTGATGG---AGAAGACAAGAAGACAGATTAA------------------- 
               
               
                 AF007269 
                 TTCGAGCCATTGATGG---AGAAGACAAGAAGACAGATTAA------------------- 
               
               
                 PPI-AtCPP 
                 TTCGAGCCACTGATGG---AGAAGACAAGAAGACAGATTAA------------------- 
               
               
                 BASF_AT2 
                 TTCGAGCCATTGATGG---AGAAGACAAGAAGACAGATTAA------------------- 
               
               
                 afc1 
                 TTCGAGCCATTGATGG---AGAAGACAAGAAGACAGATTAA------------------- 
               
               
                 BASF_AT1 
                 TTCGAGCCATTGATGG---AGAAGACAAGAAGACAGATTAA------------------- 
               
               
                 PPI-BnCPP 
                 TTCGAGCCATTGATGG---AGAAGACAAGAAGACAGATTAA------------------- 
               
               
                 BASF-Corn 
                 TGCAAGCTTTGGAAGATTCAGACGACAAAAAAGAAGATTAGTCGATCCTTGTATGAGGTT 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 ------------------------------------------------------------ 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 TACATATGGATTTTTCCCTGCCACATGCACACCGATTCAGTGCTTGGATGGTGAGGGTTT 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 ------------------------------------------------------------ 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 TGACATAGGAGTGTTGTCAAAGCTTTAGAGTGCATCTTTCGGTCAGGTGCAACAGCCTTT 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 ------------------------------------------------------------ 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 CGGTCATTGAGACATATAAGCGAATTAGCTATTAAAAAAAACAGAACTGTTGCATCAAAA 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Gm 
                 ------------------------------------------------------------ 
               
               
                 AT4g01320 
                 ------------------------------------------------------------ 
               
               
                 AF007269 
                 ------------------------------------------------------------ 
               
               
                 PPI-AtCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT2 
                 ------------------------------------------------------------ 
               
               
                 afc1 
                 ------------------------------------------------------------ 
               
               
                 BASF_AT1 
                 ------------------------------------------------------------ 
               
               
                 PPI-BnCPP 
                 ------------------------------------------------------------ 
               
               
                 BASF-Corn 
                 AAAAAAAAAAAAAAGAAACAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA 
               
               
                   
               
               
                 PPI-GmCPP 
                 ---------------------------------------------------- 
               
               
                 BASF-Gm 
                 ---------------------------------------------------- 
               
               
                 AT4g01320 
                 ---------------------------------------------------- 
               
               
                 AF007269 
                 ---------------------------------------------------- 
               
               
                 PPI-AtCPP 
                 ---------------------------------------------------- 
               
               
                 BASF_AT2 
                 ---------------------------------------------------- 
               
               
                 afc1 
                 ---------------------------------------------------- 
               
               
                 BASF_AT1 
                 ---------------------------------------------------- 
               
               
                 PPI-BnCPP 
                 ---------------------------------------------------- 
               
               
                 BASF-Corn 
                 AAAAAGTGCTCTGCGTTGTTACCACTGCTTGCCCTATAGTGATCGTATCAGA 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 18B 
               
               
                   
               
               
                 ClustalW Amino Acid Analysis of CaaX Prenyl Protease 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 1: PPI-AtCPP 
                 SEQ ID NO: 98 
               
               
                   
               
               
                 2: PPI-BnCPP 
                 SEQ ID NO: 110 
               
               
                   
               
               
                 3: PPI-GmCPP 
                 SEQ ID NO: 113 
               
               
                   
               
               
                 4: BASF_AT1 
                 SEQ ID NO: 117 
               
               
                   
               
               
                 5: BASF_AT2 
                 SEQ ID NO: 119 
               
               
                   
               
               
                 6: BASF-Corn 
                 SEQ ID NO: 121 
               
               
                   
               
               
                 7: BASF-Gm 
                 SEQ ID NO: 123 
               
               
                   
               
               
                 8: AFC1 
                 SEQ ID NO: 125 
               
               
                   
               
               
                 9: AT4g01320 
                 SEQ ID NO: 127 
               
               
                   
               
               
                 10: AF007269 
                 SEQ ID NO: 129 
               
               
                   
               
               
                 PPI-GmCPP 
                 MAFPYMEAVVGFMILMYIFETYLDVRQHRALKLPTLPKTLEG-------VISQEKFEKSR 
               
               
                 BASF-Gm 
                 MAFPYMEAVVGFMILMYIFETYLDVRQHRALKLPTLPKTLEG-------VISQEKFEKSR 
               
               
                 AF007269 
                 MAIPFMETVVGFMIVMYIFETYLDLRQLTALKLPTLPKTLI------------------- 
               
               
                 AT4g-AtCPP 
                 MAIPFMETVVGFMIVMYIFETYLDLRQLTALKLPTLPKTLVGVISQEKFEKSRAYRDIIT 
               
               
                 BASF_AT2 
                 MAIPFMETVVGFMIVMYIFETYLDLRQLTALKLPTLPKTLVG-------VISQEKFEKSR 
               
               
                 AFC1 
                 MAIPFMETVVGFMIVMYIFETYLDLRQLTALKLPTLPKTLVG-------VISQEKFEKSR 
               
               
                 BASF_AT1 
                 MAIPFMETVVGFMIVMYIFETYLDLRQLTALKLPTLPKTLVG-------VISQEKFEKSR 
               
               
                 PPI-AtCPP 
                 MAIPFMETVVGFMIVMYIFETYLDLRQLTALKLPTLPKTLVG-------VISQEKFEKSR 
               
               
                 PPI-BnCPP 
                 MAIPFMETVVGFMIVMYIFETYLDLRQLTALKLPTLPKTLVG-------VISQEKFEKSR 
               
               
                 BASF-Corn 
                 ------------------------------------------------------------ 
               
               
                   
               
               
                 PPI-GmCPP 
                 AYSLDKSHFHFVHEFVTIVTDSTILYFGVLPWFWKKSGDFMTIAGFNAENEILHTLAFLA 
               
               
                 BASF-Gm 
                 AYSLDKSHFHFVHEFVTIVTDSTILYFGVLPWFWKKSGDFMTIAGFNAENEILHTLAFLA 
               
               
                 AF007269 
                 ------------------------------------------------------------ 
               
               
                 AT4g-AtCPP 
                 ENFNICSYFHFVHEFVTILMDSAILFFGILPWFWKMSGAVLPRLGLDPENEILHTLSFLA 
               
               
                 BASF_AT2 
                 AYSLDKSYFHFVHEFVTILMDSAILFFGILPWFWKMSGAVLPRLGLDPENEILHTLSFLA 
               
               
                 AFC1 
                 AYSLDKSYFHFVHEFVTILMDSAILFFGILPWFWKMSGAVLPRLGLDPENEILHTLSFLA 
               
               
                 BASF_AT1 
                 AYSLDKSYFHFVHEFVTILMDSAILFFGILPWFWKMSGAVLPRLGLDPENEILHTLSFLA 
               
               
                 PPI-AtCPP 
                 AYSLDKSYFHFVHEFVTILMDSAILFFGILPWFWKMSGAVLPRLGLDPENEILHTLSFLA 
               
               
                 PPI-BnCPP 
                 AYSLDKSYFHFVHEFVTILMDSAILFFGILPWFWKISGGFLPMVGLDPENEILHTLSFLA 
               
               
                 BASF-Corn 
                 -------------------------------------------TRLSAENEIIHTLAFLA 
               
               
                   
               
               
                 PPI-GmCPP 
                 GLMIWSQITDLPFSLYSTFVIEARHGFNKQTPWLFFRDMLKGIFLSVIIGPPIVAAIIVI 
               
               
                 BASF-Gm 
                 GLMIWSQITDLPFSLYSTFVIEARHGFNKQTPWLFFRDMLKGIFLSVIIGPPIVAAIIVI 
               
               
                 AF007269 
                 --------TDLPFSLYSTFVIESRHGFNKQTIWMFIRDMIKGTFLSVILGPPIVAAIIFI 
               
               
                 AT4g-AtCPP 
                 GVMTWSQITDLPFSLYSTFVIESRHGFNKQTIWMFIRDMIKGTFLSVILGPPIVAAIIFI 
               
               
                 BASF_AT2 
                 GVMTWSQITDLPFSLYSTFVIESRHGFNKQTIWMFIRDMIKGTFLSVILGPPIVAAIIFI 
               
               
                 AFC1 
                 GVMTWSQITDLPFSLYSTFVIESRHGFNKQTIWMFIRDMIKGTFLSVILGPPIVAAIIFI 
               
               
                 BASF_AT1 
                 GVMTWSQITDLPFSLYSTFVIESRHGFNKQTIWMFIRDMIKGTFLSVILGPPIVAAIIFI 
               
               
                 PPI-AtCPP 
                 GVMTWSQITDLPFSLYSTFVIESRHGFNKQTIWMFIRDMIKGTFLSVILGPPIVAAIIFI 
               
               
                 PPI-BnCPP 
                 GVMTWSQITDLPFSLYSTFVIESRHGFNKQTIWMFIRDMIKGILLSVIPAPPIVAAIIVI 
               
               
                 BASF-Corn 
                 GSMVWSQITDLPFSLYSTFVIEARHGFNKQTIWLFIRDMIKGILLSMILGPPIVAAIIYI 
               
               
                   
                         **************:******** *:*:***:** :**:* .******** * 
               
               
                   
               
               
                 PPI-GmCPP 
                 VQKGGPYLAIYLWVFTFGLSIVMMTLYPVLIAPLFNKFTPLPDGQLREKIEKLASSLNYP 
               
               
                 BASF-Gm 
                 VQKGGPYLAIYLWVFTFGLSIVMMTLYPVLIAPLFNKFTPLPDGQLREKIEKLASSLNYP 
               
               
                 AF007269 
                 VQKGGPYLAIYLWAFMFILSLVMMTIYPVLIAPLFNKFTPLPDGDLREKIEKLASSLKFP 
               
               
                 AT4g-AtCPP 
                 VQKGGPYLAIYLWAFMFILSLVMMTIYPVLIAPLFNKFTPLPDGDLREKIEKLASSLKFP 
               
               
                 BASF_AT2 
                 VQKGGPYLAIYLWAFMFILSLVMMTIYPVLIAPLFNKFTPLPDGDLREKIEKLASSLKFP 
               
               
                 AFC1 
                 VQKGGPYLAIYLWAFMFILSLVMMTIYPVLIAPLFNKFTPLPDGDLREKIEKLASSLKFP 
               
               
                 BASF_AT1 
                 VQKGGPYLAIYLWAFMFILSLVMMTIYPVLIAPLFNKFTPLPDGDLREKIEKLASSLKFP 
               
               
                 PPI-AtCPP 
                 VQKGGPYLAIYLWAFMFILSLVMMTIYPVLIAPLFNKFTPLPDGDLREKIEKLASSLKFP 
               
               
                 PPI-BnCPP 
                 VQKGGPYLAIYLWAFMFILSLVMMTIYPVLIAPLFNKFTPLPDGDLREKIEKLASSLKFP 
               
               
                 BASF-Corn 
                 VQIGGPYLAIYLWGFMFVLALLMMTIYPIVIAPLFNKFTPLPEGVLREKIEKLAASLKFP 
               
               
                   
                 ** ********** * * *:::***:**::************:* *********:**::* 
               
               
                   
               
               
                 PPI-GmCPP 
                 LKKLFVVDGSTRSSHSNAYMYGFFKNKRIVPYDTLIQQCKDDEEIVAVIAHELGHWKLNH 
               
               
                 BASF-Gm 
                 LKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKDDEEIVAVIAHELGHWKLNH 
               
               
                 AF007269 
                 LKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNEDEIVAVIAHELGHWKLNH 
               
               
                 AT4g-AtCPP 
                 LKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNEDEIVAVIAHELGHWKLNH 
               
               
                 BASF_AT2 
                 LKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNEDEIVAVIAHELGHWKLNH 
               
               
                 AFC1 
                 LKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNEDEIVAVIAHELGHWKLNH 
               
               
                 BASF_AT1 
                 LKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNEDEIVAVIAHELGHWKLNH 
               
               
                 PPI-AtCPP 
                 LKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNEDEIVAVIAHELGHWKLNH 
               
               
                 PPI-BnCPP 
                 LKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCQNENEIVAVIAHELGHWKLNH 
               
               
                 BASF-Corn 
                 LKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCSNEDEIVSVIAHELGHWKLNH 
               
               
                   
                 ****************************** ********.:::***:************* 
               
               
                   
               
               
                 PPI-GmCPP 
                 TVYTFVAMQILTLLQFGGYTLVRNSADLYRSFGFDTQPVLIGLIIFQHTVIPLQQLVSFG 
               
               
                 BASF-Gm 
                 TVYTFVAMQILTLLQFGGYTLVRNSADLYRSFGFDTQPVLIGLIIFQHTVIPLQQLVSFG 
               
               
                 AF007269 
                 TTYSFIAV--------------------------------------QHTVIPLQHLVSFG 
               
               
                 AT4g-AtCPP 
                 TTYSFIAVQILAFLQFGGYTLVRNSTDLFRSFGFDTQPVLIGLIIFQHTVIPLQHLVSFG 
               
               
                 BASF_AT2 
                 TTYSFIAVQILAFLQFGGYTLVRNSTDLFRSFGFDTQPVLIGLIIFQHTVIPLQHLVSFG 
               
               
                 AFC1 
                 TTYSFIAVQILAFLQFGGYTLVRNSTDLFRSFGFDTQPVLIGLIIFQHTVIPLQHLVSFG 
               
               
                 BASF_AT1 
                 TTYSFIAVQILAFLQFGGYTLVRNSTDLFRSFGFDTQPVLIGLIIFQHTVIPLQHPVSFG 
               
               
                 PPI-AtCPP 
                 TTYSFIAVQILAFLQFGGYTLLRNSTDLFRSFGFDTQPVLIGLIIFQHTVIPLQHLVSFG 
               
               
                 PPI-BnCPP 
                 TTYSFIAVQILAFLQFGGYTLVRNSTDLFRSFGFDTQPVLIGLIIFQHTVIPLQHLVSFD 
               
               
                 BASF-Corn 
                 TVYSFVAVQLLMFLQFGGYTLVRSSKDLFGSFGFKDQPVIIGLIIFPHTIIPIQHLLSFR 
               
               
                   
                 *.*:*:*:                                       **:**:*: :** 
               
               
                   
               
               
                 PPI-GmCPP 
                 LNLVSRSFEFQADGFAKKLGYASGLRG--------------------------------- 
               
               
                 BASF-Gm 
                 LNLVSRSFEFQADGFAKKLGYASGLRG--------------------------------- 
               
               
                 AF007269 
                 LNLVSRAFEFQADAFAVKLGYAKDLR-------PALV----KLQVREDNNRTQ------- 
               
               
                 AT4g-AtCPP 
                 LNLVSRAFEFQADAFAVKLGYAKDLR-------PALV----KLQVREDNNRTQTVTSICV 
               
               
                 BASF_AT2 
                 LNLVSRAFEFQADAFAVKLGYAKDLR-------PALV----KLQE--------------- 
               
               
                 AFC1 
                 LNLVSRAFEFQADAFAVKLGYAKDLR-------PALVKLQE------------------- 
               
               
                 BASF_AT1 
                 LNLVSRAFEFQADAFAVKLGYAKDLRPTLVKLQ--------------------------- 
               
               
                 PPI-AtCPP 
                 LNLVSRAFEFQADAFAVKLDYAKDLRPALVKLQ--------------------------- 
               
               
                 PPI-BnCPP 
                 LNLVSRAFEFQADAFAVNLGYAKDLRP--------------------------------- 
               
               
                 BASF-Corn 
                 LNLVSRAFEFQADAFAKNLGYAPQLR---------------------------------- 
               
               
                   
                 ******:******.** :*.**  ** 
               
               
                   
               
               
                 PPI-GmCPP 
                 ------GLVKLQEENLSAMNTDPWYSAYHYSHPPLVERLAALDEPDKKED- 
               
               
                 BASF-Gm 
                 ------GLVKLQEENLSAMNTDPCSC------------------------- 
               
               
                 AF007269 
                 -----------TEENLSAMNTDPLYSAYHYSHPPLVERLRAIDGEDKKTD- 
               
               
                 AT4g-AtCPP 
                 THLNGFFVGILQEENLSAMNTDPLYSAYHYSHPPLVERLRAIDGEDKKTD- 
               
               
                 BASF_AT2 
                 -------------ENLSAMNTDPLYSAYHYSHPPLVERLRAIDGEDKKTD- 
               
               
                 AFC1 
                 -------------ENLSAMNTDPLHSAYHYSHPPLVERLRAIDGEDKKTD- 
               
               
                 BASF_AT1 
                 ------------EENLSAMNTDPLYSAYHYSHPPLVERLRAIDGEDKKTD- 
               
               
                 PPI-AtCPP 
                 ------------EENLSTMNTDPLYSAYHYSHPPLVERLRATDGEDKKTD- 
               
               
                 PPI-BnCPP 
                 ------ALVKLQEENLSAMNTDPLYSAYHYSHPPLVERLRAIDGEDKKTD- 
               
               
                 BASF-Corn 
                 -----AALVKLQEENLSAMNTDPWYSAYHYSHPPLVERLQALEDSDDKKED 
               
               
                   
                              ****:*****  . 
               
               
                   
               
            
           
         
       
     
     Example 32 
     Plant Transformation 
       Arabidopsis  transgenic plants were made by the method of dipping flowering plants into an  Agrobacterium  culture, based on the method of Andrew Bent in, Clough S J and Bent A F, 1998. Floral dipping: a simplified method for  Agrobacterium -mediated transformation of  Arabidopsis thaliana . Wild type plants were grown under standard conditions until the plant has both developing flowers and open flowers. The plant was inverted for 2 minutes into a solution of  Agrobacterium  culture carrying the appropriate gene construct. Plants were then left horizontal in a tray and kept covered for two days to maintain humidity and then righted and bagged to continue growth and seed development. Mature seed was bulk harvested. 
     Transformed T1 plants were selected by germination and growth on MS plates containing 50 μg/ml kanamycin. Green, kanamycin resistant (Kan R ) seedlings were identified after 2 weeks growth and transplanted to soil. Plants were bagged to ensure self fertilization and the T2 seed of each plant harvested separately. During growth of T1 plants leaf samples were harvested, DNA extracted and Southern blot and PCR analysis performed. 
     T2 seeds were analysed for Kan R  segregation. From those lines that showed a 3:1 resistant phenotype, surviving T2 plants were grown, bagged during seed set, and T3 seed harvested from each line. T3 seed was again used for Kan R  segregation analysis and those lines showing 100% Kan R  phenotype were selected as homozygous lines. Further molecular and physiological analysis was done using T3 seedlings. 
     Transgenic  Brassica napus, Glycine max  and  Zea maize  plants were produced using  Agrobacterium  mediated transformation of cotyledon petiole tissue. Seeds were sterilized as follows. Seeds were wetted with 95% ethanol for a short period of time such as 15 seconds. Approximately 30 ml of sterilizing solution I was added (70% Javex, 100 μl Tween20) and left for approximately 15 minutes. Solution I was removed and replaced with 30 ml of solution II (0.25% mecuric chloride, 100 μl Tween20) and incubated for about 10 minutes. Seeds were rinsed with at least 500 ml double distilled sterile water and stored in a sterile dish. Seeds were germinated on plates of ½ MS medium, pH 5.8, supplemented with 1% sucrose and 0.7% agar. Fully expanded cotyledons were harvested and placed on Medium I (Murashige minimal organics (MMO), 3% sucrose, 4.5 mg/L benzyl adenine (BA), 0.7% phytoagar, pH5.8). An  Agrobacterium  culture containing the nucleic acid construct of interest was grown for 2 days in AB Minimal media. The cotyledon explants were dipped such that only the cut portion of the petiole is contacted by the  Agrobacterium  solution. The explants were then embedded in Medium I and maintained for 5 days at 24° C., with 16.8 hr light dark cycles. 
     Explants were transferred to Medium II (Medium I, 300 mg/L timentin) for a further 7 days and then to Medium III (Medium II, 20 mg/L kanamycin). Any root or shoot tissue which had developed at this time was dissected away. Transfer explants to fresh plates of Medium III after 14-21 days. When regenerated shoot tissue developed the regenerated tissue was transferred to Medium IV (MMO, 3% sucrose, 1.0% phytoagar, 300 mg/L timentin, 20 mg/L 20 mg/L kanamycin). Once healthy shoot tissue developed shoot tissue dissected from any callus tissue was dipped in 10×IBA and transferred to Medium V (Murashige and Skooge (MS), 3% sucrose, 0.2 mg/L indole butyric acid (IBA), 0.7% agar, 300 mg/L timentin, 20 mg/L 20 mg/L kanamycin) for rooting. Healthy plantlets were transferred to soil. The above method, with or without modifications, is suitable for the transformation of numerous plant species including  Glycine max, Zea maize  and cotton. 
     Transgenic  Glycine max, Zea maize  and cotton can be produced using  Agrobacterium -based methods which are known to one of skill in the art. Alternatively one can use a particle or non-particle biolistic bombardment transformation method. An example of non-particle biolistic transformation is given in U.S. Patent Application 20010026941. This method has been used to produce transgenic  Glycine max  and  Zea maize  plants. Viable plants are propagated and homozygous lines are generated. Plants are tested for the presence of drought tolerance, physiological and biochemical phenotypes as described elsewhere. 
     The following table identifies the constructs and the species which they have been transformed. 
                     TABLE 19                  Transformation List                         SEQ ID NO:   Construct   Species Transformed                                 99   pBII121-AtCPP     A. thaliana ,  B. napus         100   pBII121-HP-AtCPP     A. thaliana         131   pRD29A-AtCPP     A. thaliana ,  B. napus         132   pRD29A-HP-AtCPP     A. thaliana         134   MuA-AtCPP     Glycine max ,  Zea mays                      
Non-limiting examples of vector constructs suitable for plant transformation are given in SEQ ID NO: 99, 5, 35-53.
 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 99 
                   
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatcc atggcgattcctttcatggaaaccgtcgtgggttttatgatagtgatgtacatttttga   
               
               
                   
               
               
                 
                   gacgtatttggatctgaggcaactcactgctctcaagcttccaactctcccgaaaaccttggtt 
                 
               
               
                   
               
               
                 
                   ggtgtaattagccaagagaagtttgagaaatcacgagcatacagtcttgacaaaagctattttc 
                 
               
               
                   
               
               
                 
                   actttgttcatgagtttgtaactatacttatggactctgcaattttgttctttgggatcttgcc 
                 
               
               
                   
               
               
                 
                   ttggttttggaagatgtctggagctgttttaccgaggttgggccttgatccggagaatgaaata 
                 
               
               
                   
               
               
                 
                   ctgcatactctttcattcttggctggtgttatgacatggtcacagatcactgatttgccatttt 
                 
               
               
                   
               
               
                 
                   ctttgtactcaactttcgtgatcgagtctcggcatgggttcaacaaacaaacaatatggatgtt 
                 
               
               
                   
               
               
                 
                   cattagggacatgatcaaaggaacattcctctctgtcatactaggcccacccattgttgctgcg 
                 
               
               
                   
               
               
                 
                   ataattttcatagtccagaaaggaggtccttatcttgccatctatctgtgggcattcatgttta 
                 
               
               
                   
               
               
                 
                   tcctgtctctagtgatgatgactatatacccggtcttgatagcaccgctcttcaacaaattcac 
                 
               
               
                   
               
               
                 
                   tcctcttccagatggagacctccgggagaagattgagaaacttgcttcttccctaaagtttcct 
                 
               
               
                   
               
               
                 
                   ttgaagaagctgtttgttgtcgatggatctacaaggtcaagccatagcaatgcttacatgtatg 
                 
               
               
                   
               
               
                 
                   gtttctttaagaacaaaaggattgttctttatgatacgttgattcagcagtgcaagaatgagga 
                 
               
               
                   
               
               
                 
                   tgaaattgtggcggttattgcacacgagcttggacattggaaactgaatcacactacatactcg 
                 
               
               
                   
               
               
                 
                   ttcattgcagttcaaatccttgccttcttacaatttggaggatacactcttctcagaaactcca 
                 
               
               
                   
               
               
                 
                   ctgatctcttcaggagtttcggatttgatacacagcctgttctcattggtttgatcatatttca 
                 
               
               
                   
               
               
                 
                   gcacactgtaataccactgcaacatctagtaagctttggcctgaacctcgttagtcgagcgttt 
                 
               
               
                   
               
               
                 
                   gagtttcaggctgatgcttttgctgtgaagcttgactatgcaaaagatcttcgtcctgctctag 
                 
               
               
                   
               
               
                 
                   tgaaactacaggaagagaacttatcaacaatgaacactgatccattgtactcagcttatcacta 
                 
               
               
                   
               
               
                 
                   ctcacatcctcctcttgttgaaaggcttcgagccactgatggagaagacaagaagacagattaa 
                 
               
               
                   
               
               
                 cccctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgtt 
               
               
                   
               
               
                 gccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaaca 
               
               
                   
               
               
                 tgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacattta 
               
               
                   
               
               
                 atacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatct 
               
               
                   
               
               
                 atgttactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggc 
               
               
                   
               
               
                 gttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagagg 
               
               
                   
               
               
                 cccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttct 
               
               
                   
               
               
                 tcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctcccttt 
               
               
                   
               
               
                 agggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttca 
               
               
                   
               
               
                 cgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttcttta 
               
               
                   
               
               
                 atagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgattt 
               
               
                   
               
               
                 ataagggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaacca 
               
               
                   
               
               
                 gcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgt 
               
               
                   
               
               
                 ctcactggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttg 
               
               
                   
               
               
                 tctaagcgtcaatttgtttacaccacaatatatcctgcca 
               
            
           
         
       
     
     SEQ ID NO:99 is the nucleic acid sequence of pBI121-AtCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the 35S promoter and bolded sequence is the AtCPP sense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 100 
                   
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatcctc ccaatgtccaagctcgtgtgcaataaccgccacaatttcatcctcattcttgcactg   
               
               
                   
               
               
                 
                   ctgaatcaacgtatcataaagaacaatccttttgttcttaaagaaaccatacatgtaagcattg 
                 
               
               
                   
               
               
                 
                   ctatggcttgaccttgtagatccatcgacaacaaacagcttcttcaaaggaaactttagggaag 
                 
               
               
                   
               
               
                 
                   aagcaagtttctcaatcttctcccggaggtctccatctggaagaggagtgaatttgttgaagag 
                 
               
               
                   
               
               
                 
                   cggtgctatcaagaccgggtatatagtcatcatcactagagacaggataaacatgaatgcccac 
                 
               
               
                   
               
               
                 
                   agatagatggcaagataaggacctcctttctggactatgaaaattatcgcagcaacaatgggtg 
                 
               
               
                   
               
               
                 
                   ggcctagtatgacagagaggaatgttcctttgatcatgtccctaatgaacatccatattgtttg 
                 
               
               
                   
               
               
                 
                   tttgttgaacccatgccgagactcgatcacgaaagttgagtacaaagaaaatggcaaatcagtg 
                 
               
               
                   
               
               
                 
                   atctgtgaccatgtcataacaccagccaagaatgaaagagtatgcagtatttcattctccggat 
                 
               
               
                   
               
               
                   caaggcccaacctcggtaaaagaggatcccc ATCTACCCGCTTCGCGTCGGCATCCGGTCAGTG 
               
               
                   
               
               
                 GCAGTGAAGGGCGAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCTTTGGTCGTC 
               
               
                   
               
               
                 ATGAAGATGCGGACTTGCGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGACCACGCATT 
               
               
                   
               
               
                 AATGGACTGGATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTC 
               
               
                   
               
               
                 GACTGGGCAGATGAACATGGCATCGTGGTGATTGATGAAACTGCTGCTGTCGGCTTTTCGCTCT 
               
               
                   
               
               
                 CTTTAGGCATTGGTTTCGAAGCGGGCAACAAGCCGAAAGAACTGTACAGCGAAGAGGCAGTCAA 
               
               
                   
               
               
                 CGGGGAAACTCAGCAAGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAAAACCAC 
               
               
                   
               
               
                 CCAAGCGTGGTGATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAAT 
               
               
                   
               
               
                 ATTTCGCGCCACTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAA 
               
               
                   
               
               
                 TGTAATGTTCTGCGACGCTCACACCGATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAAC 
               
               
                   
               
               
                 CGTTATTACGGATGGTATGTCCAAAGCGGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAG 
               
               
                   
               
               
                 AACTTCTGGCCTGGCAGGAGAAACTGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATG 
               
               
                   
               
               
                 GCTGGATATGTATCACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAAT 
               
               
                   
               
               
                 TTCGCCGATTTTGCGACCTCGCAAGGCATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCA 
               
               
                   
               
               
                 CTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTT 
               
               
                   
               
               
                 CGGTGAAAAACCGCAGCAGGGAGGCAAACAATGAATCAACAACTCTCCTGGCGCACCATCGTCG 
               
               
                   
               
               
                 GCTACAGCCTCGGGAATTGCTACCGAGCTC   ttttaccgaggttgggccttgatccggagaatga     
               
               
                   
               
               
                 
                   
                     aatactgcatactctttcattcttggctggtgttatgacatggtcacagatcactgatttgcca 
                   
                 
               
               
                   
               
               
                 
                   
                     ttttctttgtactcaactttcgtgatcgagtctcggcatgggttcaacaaacaaacaatatgga 
                   
                 
               
               
                   
               
               
                 
                   
                     tgttcattagggacatgatcaaaggaacattcctctctgtcatactaggcccacccattgttgc 
                   
                 
               
               
                   
               
               
                 
                   
                     tgcgataattttcatagtccagaaaggaggtccttatcttgccatctatctgtgggcattcatg 
                   
                 
               
               
                   
               
               
                 
                   
                     tttatcctgtctctagtgatgatgactatatacccggtcttgatagcaccgctcttcaacaaat 
                   
                 
               
               
                   
               
               
                 
                   
                     tcactcctcttccagatggagacctccgggagaagattgagaaacttgcttcttccctaaagtt 
                   
                 
               
               
                   
               
               
                 
                   
                     tcctttgaagaagctgtttgttgtcgatggatctacaaggtcaagccatagcaatgcttacatg 
                   
                 
               
               
                   
               
               
                 
                   
                     tatggtttctttaagaacaaaaggattgttctttatgatacgttgattcagcagtgcaagaatg 
                   
                 
               
               
                   
               
               
                     aggatgaaattgtggcggttattgcacacgagcttggacattgg   gagctcgaatttccccgatc 
               
               
                   
               
               
                 gttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattat 
               
               
                   
               
               
                 catataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttattt 
               
               
                   
               
               
                 atgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaa 
               
               
                   
               
               
                 tatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaatt 
               
               
                   
               
               
                 cactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgcct 
               
               
                   
               
               
                 tgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcc 
               
               
                   
               
               
                 caacagttgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacg 
               
               
                   
               
               
                 ttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctt 
               
               
                   
               
               
                 tacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctg 
               
               
                   
               
               
                 atagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaa 
               
               
                   
               
               
                 actggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgattt 
               
               
                   
               
               
                 cggaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaa 
               
               
                   
               
               
                 ctctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaa 
               
               
                   
               
               
                 ccaccccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaatttgttta 
               
               
                   
               
               
                 caccacaatatatcctgcca 
               
            
           
         
       
     
     SEQ ID NO:100 is the nucleic acid sequence of pBI121-HP-AtCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the 35S promoter and bolded sequence is the AtCPP anti-sense sequence. Sequence in upper case is the truncated GUS fragment. Sequence in bold and underlined is the AtCPP sense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 130 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatccTTAATCTGTCTTCTTGTCTTCTCCATCAGTGGCTCGAAGCCTTTCAACAAGAGGAGGAT 
               
               
                   
               
               
                 GTGAGTAGTGATAAGCTGAGTACAATGGATCAGTGTTCATTGTTGATAAGTTCTCTTCCTGTAG 
               
               
                   
               
               
                 TTTCACTAGAGCAGGACGAAGATCTTTTGCATAGTCAAGCTTCACAGCAAAAGCATCAGCCTGA 
               
               
                   
               
               
                 AACTCAAACGCTCGACTAACGAGGTTCAGGCCAAAGCTTACTAGATGTTGCAGTGGTATTACAG 
               
               
                   
               
               
                 TGTGCTGAAATATGATCAAACCAATGAGAACAGGCTGTGTATCAAATCCGAAACTCCTGAAGAG 
               
               
                   
               
               
                 ATCAGTGGAGTTTCTGAGAAGAGTGTATCCTCCAAATTGTAAGAAGGCAAGGATTTGAACTGCA 
               
               
                   
               
               
                 ATGAACGAGTATGTAGTGTGATTCAGTTTCCAATGTCCAAGCTCGTGTGCAATAACCGCCACAA 
               
               
                   
               
               
                 TTTCATCCTCATTCTTGCACTGCTGAATCAACGTATCATAAAGAACAATCCTTTTGTTCTTAAA 
               
               
                   
               
               
                 GAAACCATACATGTAAGCATTGCTATGGCTTGACCTTGTAGATCCATCGACAACAAACAGCTTC 
               
               
                   
               
               
                 TTCAAAGGAAACTTTAGGGAAGAAGCAAGTTTCTCAATCTTCTCCCGGAGGTCTCCATCTGGAA 
               
               
                   
               
               
                 GAGGAGTGAATTTGTTGAAGAGCGGTGCTATCAAGACCGGGTATATAGTCATCATCACTAGAGA 
               
               
                   
               
               
                 CAGGATAAACATGAATGCCCACAGATAGATGGCAAGATAAGGACCTCCTTTCTGGACTATGAAA 
               
               
                   
               
               
                 ATTATCGCAGCAACAATGGGTGGGCCTAGTATGACAGAGAGGAATGTTCCTTTGATCATGTCCC 
               
               
                   
               
               
                 TAATGAACATCCATATTGTTTGTTTGTTGAACCCATGCCGAGACTCGATCACGAAAGTTGAGTA 
               
               
                   
               
               
                 CAAAGAAAATGGCAAATCAGTGATCTGTGACCATGTCATAACACCAGCCAAGAATGAAAGAGTA 
               
               
                   
               
               
                 TGCAGTATTTCATTCTCCGGATCAAGGCCCAACCTCGGTAAAACAGCTCCAGACATCTTCCAAA 
               
               
                   
               
               
                 ACCAAGGCAAGATCCCAAAGAACAAAATTGCAGAGTCCATAAGTATAGTTACAAACTCATGAAC 
               
               
                   
               
               
                 AAAGTGAAAATAGCTTTTGTCAAGACTGTATGCTCGTGATTTCTCAAACTTCTCTTGGCTAATT 
               
               
                   
               
               
                 ACACCAACCAAGGTTTTCGGGAGAGTTGGAAGCTTGAGAGCAGTGAGTTGCCTCAGATCCAAAT 
               
               
                   
               
               
                 ACGTCTCAAAAATGTACATCACTATCATAAAACCCACGACGGTTTCCATGAAAGGAATCGCCAT 
               
               
                   
               
               
                 cccctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgtt 
               
               
                   
               
               
                 gccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaaca 
               
               
                   
               
               
                 tgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacattta 
               
               
                   
               
               
                 atacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatct 
               
               
                   
               
               
                 atgttactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggc 
               
               
                   
               
               
                 gttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagagg 
               
               
                   
               
               
                 cccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttct 
               
               
                   
               
               
                 tcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctcccttt 
               
               
                   
               
               
                 agggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttca 
               
               
                   
               
               
                 cgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttcttta 
               
               
                   
               
               
                 atagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgattt 
               
               
                   
               
               
                 ataagggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaacca 
               
               
                   
               
               
                 gcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgt 
               
               
                   
               
               
                 ctcactggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttg 
               
               
                   
               
               
                 tctaagcgtcaattt gtttacaccacaatatatcctgcca   
               
               
                   
               
            
           
         
       
     
     SEQ ID NO:130 is the nucleic acid sequence of pBI121-antisense-AtCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the 35S promoter. Sequence in upper case is the AtCPP anti-sense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 131 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaagggtttgattacttctattggaaag gactctagaggatc 
               
               
                   
               
               
                 c atggcgattcctttcatggaaaccgtcgtgggttttatgatagtgatgtacatttttgagacg   
               
               
                   
               
               
                 
                   tatttggatctgaggcaactcactgctctcaagcttccaactctcccgaaaaccttggttggtg 
                 
               
               
                   
               
               
                 
                   taattagccaagagaagtttgagaaatcacgagcatacagtcttgacaaaagctattttcactt 
                 
               
               
                   
               
               
                 
                   tgttcatgagtttgtaactatacttatggactctgcaattttgttctttgggatcttgccttgg 
                 
               
               
                   
               
               
                 
                   ttttggaagatgtctggagctgttttaccgaggttgggccttgatccggagaatgaaatactgc 
                 
               
               
                   
               
               
                 
                   atactctttcattcttggctggtgttatgacatggtcacagatcactgatttgccattttcttt 
                 
               
               
                   
               
               
                 
                   gtactcaactttcgtgatcgagtctcggcatgggttcaacaaacaaacaatatggatgttcatt 
                 
               
               
                   
               
               
                 
                   agggacatgatcaaaggaacattcctctctgtcatactaggcccacccattgttgctgcgataa 
                 
               
               
                   
               
               
                 
                   ttttcatagtccagaaaggaggtccttatcttgccatctatctgtgggcattcatgtttatcct 
                 
               
               
                   
               
               
                 
                   gtctctagtgatgatgactatatacccggtcttgatagcaccgctcttcaacaaattcactcct 
                 
               
               
                   
               
               
                 
                   cttccagatggagacctccgggagaagattgagaaacttgcttcttccctaaagtttcctttga 
                 
               
               
                   
               
               
                 
                   agaagctgtttgttgtcgatggatctacaaggtcaagccatagcaatgcttacatgtatggttt 
                 
               
               
                   
               
               
                 
                   ctttaagaacaaaaggattgttctttatgatacgttgattcagcagtgcaagaatgaggatgaa 
                 
               
               
                   
               
               
                 
                   attgtggcggttattgcacacgagcttggacattggaaactgaatcacactacatactcgttca 
                 
               
               
                   
               
               
                 
                   ttgcagttcaaatccttgccttcttacaatttggaggatacactcttctcagaaactccactga 
                 
               
               
                   
               
               
                 
                   tctcttcaggagtttcggatttgatacacagcctgttctcattggtttgatcatatttcagcac 
                 
               
               
                   
               
               
                 
                   actgtaataccactgcaacatctagtaagctttggcctgaacctcgttagtcgagcgtttgagt 
                 
               
               
                   
               
               
                 
                   ttcaggctgatgcttttgctgtgaagcttgactatgcaaaagatcttcgtcctgctctagtgaa 
                 
               
               
                   
               
               
                 
                   actacaggaagagaacttatcaacaatgaacactgatccattgtactcagcttatcactactca 
                 
               
               
                   
               
               
                   catcctcctcttgttgaaaggcttcgagccactgatggagaagacaagaagacagattaa cccc 
               
               
                   
               
               
                 tcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccg 
               
               
                   
               
               
                 gtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgta 
               
               
                   
               
               
                 atgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatac 
               
               
                   
               
               
                 gcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgt 
               
               
                   
               
               
                 tactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtta 
               
               
                   
               
               
                 cccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccg 
               
               
                   
               
               
                 caccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttcttccc 
               
               
                   
               
               
                 ttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttaggg 
               
               
                   
               
               
                 ttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgta 
               
               
                   
               
               
                 gtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatag 
               
               
                   
               
               
                 tggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataa 
               
               
                   
               
               
                 gggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgt 
               
               
                   
               
               
                 ggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctca 
               
               
                   
               
               
                 ctggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttgtcta 
               
               
                   
               
               
                 agcgtcaattt gtttacaccacaatatatcctgcca   
               
            
           
         
       
     
     SEQ ID NO:131 is the nucleic acid sequence of RD29A-AtCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the RD29A promoter. Sequence in bold is the AtCPP sense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 132 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaagggtttgattacttctattggaaag gactctagaggatc 
               
               
                   
               
               
                 ctc ccaatgtccaagctcgtgtgcaataaccgccacaatttcatcctcattcttgcactgctga   
               
               
                   
               
               
                 
                   atcaacgtatcataaagaacaatccttttgttcttaaagaaaccatacatgtaagcattgctat 
                 
               
               
                   
               
               
                 
                   ggcttgaccttgtagatccatcgacaacaaacagcttcttcaaaggaaactttagggaagaagc 
                 
               
               
                   
               
               
                 
                   aagtttctcaatcttctcccggaggtctccatctggaagaggagtgaatttgttgaagagcggt 
                 
               
               
                   
               
               
                 
                   gctatcaagaccgggtatatagtcatcatcactagagacaggataaacatgaatgcccacagat 
                 
               
               
                   
               
               
                 
                   agatggcaagataaggacctcctttctggactatgaaaattatcgcagcaacaatgggtgggcc 
                 
               
               
                   
               
               
                 
                   tagtatgacagagaggaatgttcctttgatcatgtccctaatgaacatccatattgtttgtttg 
                 
               
               
                   
               
               
                 
                   ttgaacccatgccgagactcgatcacgaaagttgagtacaaagaaaatggcaaatcagtgatct 
                 
               
               
                   
               
               
                 
                   gtgaccatgtcataacaccagccaagaatgaaagagtatgcagtatttcattctccggatcaag 
                 
               
               
                   
               
               
                   gcccaacctcggtaaaa gaggatccccATCTACCCGCTTCGCGTCGGCATCCGGTCAGTGGCAG 
               
               
                   
               
               
                 TGAAGGGCGAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCTTTGGTCGTCATGA 
               
               
                   
               
               
                 AGATGCGGACTTGCGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGACCACGCATTAATG 
               
               
                   
               
               
                 GACTGGATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACT 
               
               
                   
               
               
                 GGGCAGATGAACATGGCATCGTGGTGATTGATGAAACTGCTGCTGTCGGCTTTTCGCTCTCTTT 
               
               
                   
               
               
                 AGGCATTGGTTTCGAAGCGGGCAACAAGCCGAAAGAACTGTACAGCGAAGAGGCAGTCAACGGG 
               
               
                   
               
               
                 GAAACTCAGCAAGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAAAACCACCCAA 
               
               
                   
               
               
                 GCGTGGTGATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTT 
               
               
                   
               
               
                 CGCGCCACTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTA 
               
               
                   
               
               
                 ATGTTCTGCGACGCTCACACCGATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTT 
               
               
                   
               
               
                 ATTACGGATGGTATGTCCAAAGCGGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACT 
               
               
                   
               
               
                 TCTGGCCTGGCAGGAGAAACTGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTG 
               
               
                   
               
               
                 GATATGTATCACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCG 
               
               
                   
               
               
                 CCGATTTTGCGACCTCGCAAGGCATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCG 
               
               
                   
               
               
                 CGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGT 
               
               
                   
               
               
                 GAAAAACCGCAGCAGGGAGGCAAACAATGAATCAACAACTCTCCTGGCGCACCATCGTCGGCTA 
               
               
                   
               
               
                 CAGCCTCGGGAATTGCTACCGAGCTC   ttttaccgaggttgggccttgatccggagaatgaaata     
               
               
                   
               
               
                 
                   
                     ctgcatactctttcattcttggctggtgttatgacatggtcacagatcactgatttgccatttt 
                   
                 
               
               
                   
               
               
                 
                   
                     ctttgtactcaactttcgtgatcgagtctcggcatgggttcaacaaacaaacaatatggatgtt 
                   
                 
               
               
                   
               
               
                 
                   
                     cattagggacatgatcaaaggaacattcctctctgtcatactaggcccacccattgttgctgcg 
                   
                 
               
               
                   
               
               
                 
                   
                     ataattttcatagtccagaaaggaggtccttatcttgccatctatctgtgggcattcatgttta 
                   
                 
               
               
                   
               
               
                 
                   
                     tcctgtctctagtgatgatgactatatacccggtcttgatagcaccgctcttcaacaaattcac 
                   
                 
               
               
                   
               
               
                 
                   
                     tcctcttccagatggagacctccgggagaagattgagaaacttgcttcttccctaaagtttcct 
                   
                 
               
               
                   
               
               
                 
                   
                     ttgaagaagctgtttgttgtcgatggatctacaaggtcaagccatagcaatgcttacatgtatg 
                   
                 
               
               
                   
               
               
                 
                   
                     gtttctttaagaacaaaaggattgttctttatgatacgttgattcagcagtgcaagaatgagga 
                   
                 
               
               
                   
               
               
                     tgaaattgtggcggttattgcacacgagcttggacattgggag   ctcgaatttccccgatcgttc 
               
               
                   
               
               
                 aaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcata 
               
               
                   
               
               
                 taatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatga 
               
               
                   
               
               
                 gatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatata 
               
               
                   
               
               
                 gcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcact 
               
               
                   
               
               
                 ggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgca 
               
               
                   
               
               
                 gcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaac 
               
               
                   
               
               
                 agttgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcg 
               
               
                   
               
               
                 ccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacg 
               
               
                   
               
               
                 gcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatag 
               
               
                   
               
               
                 acggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactg 
               
               
                   
               
               
                 gaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcgga 
               
               
                   
               
               
                 accaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactct 
               
               
                   
               
               
                 ctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccac 
               
               
                   
               
               
                 cccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttacacc   
               
               
                   
               
               
                 
                   acaatatatcctgcca 
                 
               
            
           
         
       
     
     SEQ ID NO:132 is the nucleic acid sequence of RD29A-HP-AtCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the RD29A promoter. Sequence in bold is the AtCPP anti-sense sequence. Upper case sequence represents the truncated GUS fragment. Bold and underlined sequence represents the  A. thaliana  CaaX prenyl protease sense fragment. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 133 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaagggtttgattacttctattggaaag gactctagaggatc 
               
               
                   
               
               
                 cTTAATCTGTCTTCTTGTCTTCTCCATCAGTGGCTCGAAGCCTTTCAACAAGAGGAGGATGTGA 
               
               
                   
               
               
                 GTAGTGATAAGCTGAGTACAATGGATCAGTGTTCATTGTTGATAAGTTCTCTTCCTGTAGTTTC 
               
               
                   
               
               
                 ACTAGAGCAGGACGAAGATCTTTTGCATAGTCAAGCTTCACAGCAAAAGCATCAGCCTGAAACT 
               
               
                   
               
               
                 CAAACGCTCGACTAACGAGGTTCAGGCCAAAGCTTACTAGATGTTGCAGTGGTATTACAGTGTG 
               
               
                   
               
               
                 CTGAAATATGATCAAACCAATGAGAACAGGCTGTGTATCAAATCCGAAACTCCTGAAGAGATCA 
               
               
                   
               
               
                 GTGGAGTTTCTGAGAAGAGTGTATCCTCCAAATTGTAAGAAGGCAAGGATTTGAACTGCAATGA 
               
               
                   
               
               
                 ACGAGTATGTAGTGTGATTCAGTTTCCAATGTCCAAGCTCGTGTGCAATAACCGCCACAATTTC 
               
               
                   
               
               
                 ATCCTCATTCTTGCACTGCTGAATCAACGTATCATAAAGAACAATCCTTTTGTTCTTAAAGAAA 
               
               
                   
               
               
                 CCATACATGTAAGCATTGCTATGGCTTGACCTTGTAGATCCATCGACAACAAACAGCTTCTTCA 
               
               
                   
               
               
                 AAGGAAACTTTAGGGAAGAAGCAAGTTTCTCAATCTTCTCCCGGAGGTCTCCATCTGGAAGAGG 
               
               
                   
               
               
                 AGTGAATTTGTTGAAGAGCGGTGCTATCAAGACCGGGTATATAGTCATCATCACTAGAGACAGG 
               
               
                   
               
               
                 ATAAACATGAATGCCCACAGATAGATGGCAAGATAAGGACCTCCTTTCTGGACTATGAAAATTA 
               
               
                   
               
               
                 TCGCAGCAACAATGGGTGGGCCTAGTATGACAGAGAGGAATGTTCCTTTGATCATGTCCCTAAT 
               
               
                   
               
               
                 GAACATCCATATTGTTTGTTTGTTGAACCCATGCCGAGACTCGATCACGAAAGTTGAGTACAAA 
               
               
                   
               
               
                 GAAAATGGCAAATCAGTGATCTGTGACCATGTCATAACACCAGCCAAGAATGAAAGAGTATGCA 
               
               
                   
               
               
                 GTATTTCATTCTCCGGATCAAGGCCCAACCTCGGTAAAACAGCTCCAGACATCTTCCAAAACCA 
               
               
                   
               
               
                 AGGCAAGATCCCAAAGAACAAAATTGCAGAGTCCATAAGTATAGTTACAAACTCATGAACAAAG 
               
               
                   
               
               
                 TGAAAATAGCTTTTGTCAAGACTGTATGCTCGTGATTTCTCAAACTTCTCTTGGCTAATTACAC 
               
               
                   
               
               
                 CAACCAAGGTTTTCGGGAGAGTTGGAAGCTTGAGAGCAGTGAGTTGCCTCAGATCCAAATACGT 
               
               
                   
               
               
                 CTCAAAAATGTACATCACTATCATAAAACCCACGACGGTTTCCATGAAAGGAATCGCCATcccc 
               
               
                   
               
               
                 tcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccg 
               
               
                   
               
               
                 gtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgta 
               
               
                   
               
               
                 atgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatac 
               
               
                   
               
               
                 gcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgt 
               
               
                   
               
               
                 tactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtta 
               
               
                   
               
               
                 cccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccg 
               
               
                   
               
               
                 caccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttcttccc 
               
               
                   
               
               
                 ttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttaggg 
               
               
                   
               
               
                 ttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgta 
               
               
                   
               
               
                 gtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatag 
               
               
                   
               
               
                 tggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataa 
               
               
                   
               
               
                 gggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgt 
               
               
                   
               
               
                 ggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctca 
               
               
                   
               
               
                 ctggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttgtcta 
               
               
                   
               
               
                 agcgtcaatttg tttacaccacaatatatcctgcca   
               
            
           
         
       
     
     SEQ ID NO:133 is the nucleic acid sequence of RD29A-antisense-AtCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the RD29A promoter. Sequence in upper case sequence is the AtCPP anti-sense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 134 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagctGGGAAATTTTTCGCCAGTTCTAAATATCCGGAAACCTCTTGGGATGCCATTGCCCATCT 
               
               
                   
               
               
                 ATCTGTAATTTATTGACGAAATAGACGAAAAGGAAGGTGGCTCCTATAAAGCACATCATTGCGA 
               
               
                   
               
               
                 TAACAGAAAGGCCATTGTTGAAGATACCTCTGCTGACATTGGTCCCCAAGTGGAAGCACCACCC 
               
               
                   
               
               
                 CATGAGGAGCACCGTGGAGTAAGAAGACGTTCGAGCCACGTCGAAAAAGCAAGTGTGTTGATGT 
               
               
                   
               
               
                 AGTATCTCCATTGACGTAAGGGATGACGCACAATCCAACTATCCATCGCAAGACCATTGCTCTA 
               
               
                   
               
               
                 TATAAGAAAGTTAATATCATTTCGAGTGGCCACGCTGAGGGGGATCC atggcgattcctttcat   
               
               
                   
               
               
                 
                   ggaaaccgtcgtgggttttatgatagtgatgtacatttttgagacgtatttggatctgaggcaa 
                 
               
               
                   
               
               
                 
                   ctcactgctctcaagcttccaactctcccgaaaaccttggttggtgtaattagccaagagaagt 
                 
               
               
                   
               
               
                 
                   ttgagaaatcacgagcatacagtcttgacaaaagctattttcactttgttcatgagtttgtaac 
                 
               
               
                   
               
               
                 
                   tatacttatggactctgcaattttgttctttgggatcttgccttggttttggaagatgtctgga 
                 
               
               
                   
               
               
                 
                   gctgttttaccgaggttgggccttgatccggagaatgaaatactgcatactctttcattcttgg 
                 
               
               
                   
               
               
                 
                   ctggtgttatgacatggtcacagatcactgatttgccattttctttgtactcaactttcgtgat 
                 
               
               
                   
               
               
                 
                   cgagtctcggcatgggttcaacaaacaaacaatatggatgttcattagggacatgatcaaagga 
                 
               
               
                   
               
               
                 
                   acattcctctctgtcatactaggcccacccattgttgctgcgataattttcatagtccagaaag 
                 
               
               
                   
               
               
                 
                   gaggtccttatcttgccatctatctgtgggcattcatgtttatcctgtctctagtgatgatgac 
                 
               
               
                   
               
               
                 
                   tatatacccggtcttgatagcaccgctcttcaacaaattcactcctcttccagatggagacctc 
                 
               
               
                   
               
               
                 
                   cgggagaagattgagaaacttgcttcttccctaaagtttcctttgaagaagctgtttgttgtcg 
                 
               
               
                   
               
               
                 
                   atggatctacaaggtcaagccatagcaatgcttacatgtatggtttctttaagaacaaaaggat 
                 
               
               
                   
               
               
                 
                   tgttctttatgatacgttgattcagcagtgcaagaatgaggatgaaattgtggcggttattgca 
                 
               
               
                   
               
               
                 
                   cacgagcttggacattggaaactgaatcacactacatactcgttcattgcagttcaaatccttg 
                 
               
               
                   
               
               
                 
                   ccttcttacaatttggaggatacactcttctcagaaactccactgatctcttcaggagtttcgg 
                 
               
               
                   
               
               
                 
                   atttgatacacagcctgttctcattggtttgatcatatttcagcacactgtaataccactgcaa 
                 
               
               
                   
               
               
                 
                   catctagtaagctttggcctgaacctcgttagtcgagcgtttgagtttcaggctgatgcttttg 
                 
               
               
                   
               
               
                 
                   ctgtgaagcttgactatgcaaaagatcttcgtcctgctctagtgaaactacaggaagagaactt 
                 
               
               
                   
               
               
                 
                   atcaacaatgaacactgatccattgtactcagcttatcactactcacatcctcctcttgttgaa 
                 
               
               
                   
               
               
                   aggcttcgagccactgatggagaagacaagaagacagattaa cccctcgaatttccccgatcgt 
               
               
                   
               
               
                 tcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatca 
               
               
                   
               
               
                 tataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttat 
               
               
                   
               
               
                 gagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaata 
               
               
                   
               
               
                 tagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattca 
               
               
                   
               
               
                 ctggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttg 
               
               
                   
               
               
                 cagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttccca 
               
               
                   
               
               
                 acagttgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgtt 
               
               
                   
               
               
                 cgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgcttta 
               
               
                   
               
               
                 cggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgat 
               
               
                   
               
               
                 agacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaac 
               
               
                   
               
               
                 tggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcg 
               
               
                   
               
               
                 gaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaact 
               
               
                   
               
               
                 ctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaacc 
               
               
                   
               
               
                 accccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttaca   
               
               
                   
               
               
                 
                   ccacaatatatcctgcca 
                 
               
            
           
         
       
     
     SEQ ID NO:134 is the nucleic acid sequence of MuA-AtCPP. Italicized sequences are the right and left border repeats. Sequence in upper case is the MuA promoter. The  A. thaliana  CaaX prenyl protease sense sequence is in bold. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 135 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagctGGGAAATTTTTCGCCAGTTCTAAATATCCGGAAACCTCTTGGGATGCCATTGCCCATCT 
               
               
                   
               
               
                 ATCTGTAATTTATTGACGAAATAGACGAAAAGGAAGGTGGCTCCTATAAAGCACATCATTGCGA 
               
               
                   
               
               
                 TAACAGAAAGGCCATTGTTGAAGATACCTCTGCTGACATTGGTCCCCAAGTGGAAGCACCACCC 
               
               
                   
               
               
                 CATGAGGAGCACCGTGGAGTAAGAAGACGTTCGAGCCACGTCGAAAAAGCAAGTGTGTTGATGT 
               
               
                   
               
               
                 AGTATCTCCATTGACGTAAGGGATGACGCACAATCCAACTATCCATCGCAAGACCATTGCTCTA 
               
               
                   
               
               
                 TATAAGAAAGTTAATATCATTTCGAGTGGCCACGCTGAGGGGGATCGGG ATGGCGTTTCCCTAC   
               
               
                   
               
               
                 
                   ATGGAAGCCGTTGTCGGATTTATGATATTAATGTACATTTTTGAAACTTACTTGGATGTGCGAC 
                 
               
               
                   
               
               
                 
                   AACATAGGGCCCTCAAACTTCCTACTCTTCCAAAGACTTTAGAGGGTGTTATCAGCCAAGAGAA 
                 
               
               
                   
               
               
                 
                   ATTTGAGAAATCTAGAGCCTATAGTCTTGATAAAAGCCACTTCCATTTTGTTCACGAGTTTGTG 
                 
               
               
                   
               
               
                 
                   ACAATAGTGACAGACTCTACAATTTTGTACTTTGGGGTATTGCCCTGGTTTTGGAAGAAATCAG 
                 
               
               
                   
               
               
                 
                   GAGATTTTATGACAATAGCTGGTTTCAATGCTGAGAATGAAATACTGCATACCCTTGCCTTCTT 
                 
               
               
                   
               
               
                 
                   AGCAGGGCTGATGATTTGGTCACAGATAACAGATTTGCCCTTTTCTCTGTACTCAACTTTTGTG 
                 
               
               
                   
               
               
                 
                   ATTGAGGCCCGTCATGGTTTTAATAAGCAAACACCATGGTTATTCTTTAGGGACATGCTTAAAG 
                 
               
               
                   
               
               
                 
                   GATTTTCCTTTCTGTAATAATTGGTCCACCTATTGTGGCTGCAATCATTGTAATAGTACAGAA 
                 
               
               
                   
               
               
                 
                   AGGAGGTCCATACTTGGCCATCTATCTTTGGGTTTTTACGTTTGGTCTTTCTATTGTGATGATG 
                 
               
               
                   
               
               
                 
                   ACCCTTTATCCAGTACTAATAGCTCCACTCTTCAATAAGTTCACTCCACTTCCAGATGGTCAAC 
                 
               
               
                   
               
               
                 
                   TCAGGGAGAAAATCGAGAAACTTGCTTCCTCCCTCAACTATCCGTTAAAGAAACTATTTGTTGT 
                 
               
               
                   
               
               
                 
                   CGATGGATCCACAAGATCAAGTCACAGCAATGCCTATATGTATGGATTCTTCAAGAACAAGAGG 
                 
               
               
                   
               
               
                 
                   ATTGTCCCTTATGACACATTAATTCAACAGTGCAAAGACGATGAGGAAATTGTTGCTGTTATTG 
                 
               
               
                   
               
               
                 
                   CCCATGAGTTGGGACACTGGAAGCTCAACCATACTGTGTACACATTTGTTGCTATGCAGATTCT 
                 
               
               
                   
               
               
                 
                   TACACTTCTACAATTTGGAGGATATACACTAGTGCGAAATTCAGCTGATCTGTATCGAAGCTTT 
                 
               
               
                   
               
               
                 
                   GGGTTTGATACGCAGCCAGTCCTCATTGGGCTCATCATATTTCAGCATACTGTAATCCCACTTC 
                 
               
               
                   
               
               
                 
                   AGCAATTGGTCAGCTTTGGTCTGAACCTAGTCAGCCGATCATTTGAATTTCAGGCTGATGGCTT 
                 
               
               
                   
               
               
                 
                   TGCCAAGAAGCTTGGATATGCATCTGGATTACGCGGTGGTCTTGTGAAACTACAGGAGGAGAAT 
                 
               
               
                   
               
               
                 
                   CTGTCAGCTATGAATACAGATCCTTGGTACTCTGCTTATCACTATTCTCATCCTCCCCTTGTTG 
                 
               
               
                   
               
               
                   AAAGATTGGCCGCGCTGGACGAACCGGATAAGAAGGAAGACTAA gagctcgaatttccccgatc 
               
               
                   
               
               
                 gttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattat 
               
               
                   
               
               
                 catataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttattt 
               
               
                   
               
               
                 atgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaa 
               
               
                   
               
               
                 tatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaatt 
               
               
                   
               
               
                 cactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgcct 
               
               
                   
               
               
                 tgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcc 
               
               
                   
               
               
                 caacagttgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacg 
               
               
                   
               
               
                 ttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctt 
               
               
                   
               
               
                 tacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctg 
               
               
                   
               
               
                 atagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaa 
               
               
                   
               
               
                 actggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgattt 
               
               
                   
               
               
                 cggaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaa 
               
               
                   
               
               
                 ctctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaa 
               
               
                   
               
               
                 ccaccccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gttta   
               
               
                   
               
               
                 
                   caccacaatatatcctgcca 
                 
               
            
           
         
       
     
     SEQ ID NO:135 is the nucleic acid sequence of MuA-GmCPP. Italicized sequences are the right and left border repeats. Sequence in upper case is the MuA promoter. The  G. max  CaaX prenyl protease sense sequence is in upper case and bold. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 136 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatccccggg atggcgtttccctacatggaagccgttgtcggatttatgatattaatgtacatt   
               
               
                   
               
               
                 
                   tttgaaacttacttggatgtgcgacaacatagggccctcaaacttcctactcttccaaagactt 
                 
               
               
                   
               
               
                 
                   tagagggtgttatcagccaagagaaatttgagaaatctagagcctatagtcttgataaaagcca 
                 
               
               
                   
               
               
                 
                   cttccattttgttcacgagtttgtgacaatagtgacagactctacaattttgtactttggggta 
                 
               
               
                   
               
               
                 
                   ttgccctggttttggaagaaatcaggagattttatgacaatagctggtttcaatgctgagaatg 
                 
               
               
                   
               
               
                 
                   aaatactgcatacccttgccttcttagcagggctgatgatttggtcacagataacagatttgcc 
                 
               
               
                   
               
               
                 
                   cttttctctgtactcaacttttgtgattgaggcccgtcatggttttaataagcaaacaccatgg 
                 
               
               
                   
               
               
                 
                   ttattctttagggacatgcttaaaggaattttcctttctgtaataattggtccacctattgtgg 
                 
               
               
                   
               
               
                 
                   ctgcaatcattgtaatagtacagaaaggaggtccatacttggccatctatctttgggtttttac 
                 
               
               
                   
               
               
                 
                   gtttggtctttctattgtgatgatgaccctttatccagtactaatagctccactcttcaataag 
                 
               
               
                   
               
               
                 
                   ttcactccacttccagatggtcaactcagggagaaaatcgagaaacttgcttcctccctcaact 
                 
               
               
                   
               
               
                 
                   atccgttaaagaaactatttgttgtcgatggatccacaagatcaagtcacagcaatgcctatat 
                 
               
               
                   
               
               
                 
                   gtatggattcttcaagaacaagaggattgtcccttatgacacattaattcaacagtgcaaagac 
                 
               
               
                   
               
               
                 
                   gatgaggaaattgttgctgttattgcccatgagttgggacactggaagctcaaccatactgtgt 
                 
               
               
                   
               
               
                 
                   acacatttgttgctatgcagattcttacacttctacaatttggaggatatacactagtgcgaaa 
                 
               
               
                   
               
               
                 
                   ttcagctgatctgtatcgaagctttgggtttgatacgcagccagtcctcattgggctcatcata 
                 
               
               
                   
               
               
                 
                   tttcagcatactgtaatcccacttcagcaattggtcagctttggtctgaacctagtcagccgat 
                 
               
               
                   
               
               
                 
                   catttgaatttcaggctgatggctttgccaagaagcttggatatgcatctggattacgcggtgg 
                 
               
               
                   
               
               
                 
                   tcttgtgaaactacaggaggagaatctgtcagctatgaatacagatccttggtactctgcttat 
                 
               
               
                   
               
               
                 
                   cactattctcatcctccccttgttgaaagattggccgcgctggacgaaccggataagaaggaag 
                 
               
               
                   
               
               
                   actaa gagctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatc 
               
               
                   
               
               
                 ctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataat 
               
               
                   
               
               
                 taacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatac 
               
               
                   
               
               
                 atttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgt 
               
               
                   
               
               
                 catctatgttactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaacc 
               
               
                   
               
               
                 ctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcga 
               
               
                   
               
               
                 agaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgc 
               
               
                   
               
               
                 tttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctc 
               
               
                   
               
               
                 cctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatg 
               
               
                   
               
               
                 gttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgtt 
               
               
                   
               
               
                 ctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattctttt 
               
               
                   
               
               
                 gatttataagggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggca 
               
               
                   
               
               
                 aaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg 
               
               
                   
               
               
                 cccgtctcactggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttatta 
               
               
                   
               
               
                 agttgtctaagcgtcaattt gtttacaccacaatatatcctgcca   
               
            
           
         
       
     
     SEQ ID NO:135 is the nucleic acid sequence of pBI121-GmCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the 35S promoter. The  G. max  CaaX prenyl protease sense sequence is in bold. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 137 
                   
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctaga c   
               
               
                   
               
               
                 
                   cggttcgtccagcgcggccaatctttcaacaaggggaggatgagaatagtgataagcagagtac 
                 
               
               
                   
               
               
                 
                   caaggatctgtattcatagctgacagattctcctcctgtagtttcacaagaccaccgcgtaatc 
                 
               
               
                   
               
               
                 
                   cagatgcatatccaagcttcttggcaaagccatcagcctgaaattcaaatgatcggctgactag 
                 
               
               
                   
               
               
                 
                   gttcagaccaaagctgaccaattgctgaagtgggattacagtatgctgaaatatgatgagccca 
                 
               
               
                   
               
               
                 
                   atgaggactggctgcgtatcaaacccaaagcttcgatacagatcagctgaatttcgcactagtg 
                 
               
               
                   
               
               
                 
                   tatatcctccaaattgtagaagtgtaagaatctgcatagcaacaaatgtgtacacagtatggtt 
                 
               
               
                   
               
               
                 
                   gagcttccagtgtcccaactcatgggcaataacagcaacaatttcctcatcgtctttgcactgt 
                 
               
               
                   
               
               
                 
                   tgaattaatgtgtcataagggacaatcctcttgttcttgaagaatccatacatataggcattgc 
                 
               
               
                   
               
               
                   tgtgacttgatcttgt ggatccccATCTACCCGCTTCGCGTCGGCATCCGGTCAGTGGCAGTGA 
               
               
                   
               
               
                 AGGGCGAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCTTTGGTCGTCATGAAGA 
               
               
                   
               
               
                 TGCGGACTTGCGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGACCACGCATTAATGGAC 
               
               
                   
               
               
                 TGGATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGG 
               
               
                   
               
               
                 CAGATGAACATGGCATCGTGGTGATTGATGAAACTGCTGCTGTCGGCTTTTCGCTCTCTTTAGG 
               
               
                   
               
               
                 CATTGGTTTCGAAGCGGGCAACAAGCCGAAAGAACTGTACAGCGAAGAGGCAGTCAACGGGGAA 
               
               
                   
               
               
                 ACTCAGCAAGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCG 
               
               
                   
               
               
                 TGGTGATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGC 
               
               
                   
               
               
                 GCCACTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATG 
               
               
                   
               
               
                 TTCTGCGACGCTCACACCGATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATT 
               
               
                   
               
               
                 ACGGATGGTATGTCCAAAGCGGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCT 
               
               
                   
               
               
                 GGCCTGGCAGGAGAAACTGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGAT 
               
               
                   
               
               
                 ATGTATCACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCG 
               
               
                   
               
               
                 ATTTTGCGACCTCGCAAGGCATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGA 
               
               
                   
               
               
                 CCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAA 
               
               
                   
               
               
                 AAACCGCAGCAGGGAGGCAAACAATGAatcaacaactctcctggcgcaccatcgtcggctacag 
               
               
                   
               
               
                 cctcgggaattgctaccgagctc   acaagatcaagtcacagcaatgcctatatgtatggattctt     
               
               
                   
               
               
                 
                   
                     caagaacaagaggattgtcccttatgacacattaattcaacagtgcaaagacgatgaggaaatt 
                   
                 
               
               
                   
               
               
                 
                   
                     gttgctgttattgcccatgagttgggacactggaagctcaaccatactgtgtacacatttgttg 
                   
                 
               
               
                   
               
               
                 
                   
                     ctatgcagattcttacacttctacaatttggaggatatacactagtgcgaaattcagctgatct 
                   
                 
               
               
                   
               
               
                 
                   
                     gtatcgaagctttgggtttgatacgcagccagtcctcattgggctcatcatatttcagcatact 
                   
                 
               
               
                   
               
               
                 
                   
                     gtaatcccacttcagcaattggtcagctttggtctgaacctagtcagccgatcatttgaatttc 
                   
                 
               
               
                   
               
               
                 
                   
                     aggctgatggctttgccaagaagcttggatatgcatctggattacgcggtggtcttgtgaaact 
                   
                 
               
               
                   
               
               
                 
                   
                     acaggaggagaatctgtcagctatgaatacagatccttggtactctgcttatcactattctcat 
                   
                 
               
               
                   
               
               
                     cctccccttgttgaaagattggccgcgctggacgaaccgg   gagctcgaatttccccgatcgttc 
               
               
                   
               
               
                 aaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcata 
               
               
                   
               
               
                 taatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatga 
               
               
                   
               
               
                 gatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatata 
               
               
                   
               
               
                 gcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcact 
               
               
                   
               
               
                 ggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgca 
               
               
                   
               
               
                 gcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaac 
               
               
                   
               
               
                 agttgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcg 
               
               
                   
               
               
                 ccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacg 
               
               
                   
               
               
                 gcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatag 
               
               
                   
               
               
                 acggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactg 
               
               
                   
               
               
                 gaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcgga 
               
               
                   
               
               
                 accaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactct 
               
               
                   
               
               
                 ctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccac 
               
               
                   
               
               
                 cccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttacacc   
               
               
                   
               
               
                 
                   acaatatatcctgcca 
                 
               
            
           
         
       
     
     SEQ ID NO:137 is the nucleic acid sequence of pBI121-HP-GmCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the 35S promoter. Bold sequence is the antisense prenyl protease fragment of  G. max . Bold and underlined sequence is the  G. max  sense prenyl protease fragment and sequence in upper case is the truncated GUS fragment. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 138 
                   
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatccccggg ttagtcttccttcttatccggttcgtccagcgcggccaatctttcaacaagggg   
               
               
                   
               
               
                 
                   aggatgagaatagtgataagcagagtaccaaggatctgtattcatagctgacagattctcctcc 
                 
               
               
                   
               
               
                 
                   tgtagtttcacaagaccaccgcgtaatccagatgcatatccaagcttcttggcaaagccatcag 
                 
               
               
                   
               
               
                 
                   cctgaaattcaaatgatcggctgactaggttcagaccaaagctgaccaattgctgaagtgggat 
                 
               
               
                   
               
               
                 
                   tacagtatgctgaaatatgatgagcccaatgaggactggctgcgtatcaaacccaaagcttcga 
                 
               
               
                   
               
               
                 
                   tacagatcagctgaatttcgcactagtgtatatcctccaaattgtagaagtgtaagaatctgca 
                 
               
               
                   
               
               
                 
                   tagcaacaaatgtgtacacagtatggttgagcttccagtgtcccaactcatgggcaataacagc 
                 
               
               
                   
               
               
                 
                   aacaatttcctcatcgtctttgcactgttgaattaatgtgtcataagggacaatcctcttgttc 
                 
               
               
                   
               
               
                 
                   ttgaagaatccatacatataggcattgctgtgacttgatcttgtggatccatcgacaacaaata 
                 
               
               
                   
               
               
                 
                   gtttctttaacggatagttgagggaggaagcaagtttctcgattttctccctgagttgaccatc 
                 
               
               
                   
               
               
                 
                   tggaagtggagtgaacttattgaagagtggagctattagtactggataaagggtcatcatcaca 
                 
               
               
                   
               
               
                 
                   atagaaagaccaaacgtaaaaacccaaagatagatggccaagtatggacctcctttctgtacta 
                 
               
               
                   
               
               
                 
                   ttacaatgattgcagccacaataggtggaccaattattacagaaaggaaaattcctttaagcat 
                 
               
               
                   
               
               
                 
                   gtccctaaagaataaccatggtgtttgcttattaaaaccatgacgggcctcaatcacaaaagtt 
                 
               
               
                   
               
               
                 
                   gagtacagagaaaagggcaaatctgttatctgtgaccaaatcatcagccctgctaagaaggcaa 
                 
               
               
                   
               
               
                 
                   gggtatgcagtatttcattctcagcattgaaaccagctattgtcataaaatctcctgatttctt 
                 
               
               
                   
               
               
                 
                   ccaaaaccagggcaataccccaaagtacaaaattgtagagtctgtcactattgtcacaaactcg 
                 
               
               
                   
               
               
                 
                   tgaacaaaatggaagtggcttttatcaagactataggctctagatttctcaaatttctcttggc 
                 
               
               
                   
               
               
                 
                   tgataacaccctctaaagtctttggaagagtaggaagtttgagggccctatgttgtcgcacatc 
                 
               
               
                   
               
               
                 
                   caagtaagtttcaaaaatgtacattaatatcataaatccgacaacggcttccatgtagggaaac 
                 
               
               
                   
               
               
                   gccat gagctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatc 
               
               
                   
               
               
                 ctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataat 
               
               
                   
               
               
                 taacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatac 
               
               
                   
               
               
                 atttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgt 
               
               
                   
               
               
                 catctatgttactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaacc 
               
               
                   
               
               
                 ctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcga 
               
               
                   
               
               
                 agaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgc 
               
               
                   
               
               
                 tttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctc 
               
               
                   
               
               
                 cctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatg 
               
               
                   
               
               
                 gttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgtt 
               
               
                   
               
               
                 ctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattctttt 
               
               
                   
               
               
                 gatttataagggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggca 
               
               
                   
               
               
                 aaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg 
               
               
                   
               
               
                 cccgtctcactggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttatta 
               
               
                   
               
               
                 agttgtctaagcgtcaattt gtttacaccacaatatatcctgcca   
               
            
           
         
       
     
     SEQ ID NO:138 is the nucleic acid sequence of pBI121-antisense-GmCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the 35S promoter. Sequence in bold is the GmCPP anti-sense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 139 
                   
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaagggtttgattacttctattggaaag gactctagaggatc 
               
               
                   
               
               
                 cccggg atggcgtttccctacatggaagccgttgtcggatttatgatattaatgtacatttttg   
               
               
                   
               
               
                 
                   aaacttacttggatgtgcgacaacatagggccctcaaacttcctactcttccaaagactttaga 
                 
               
               
                   
               
               
                 
                   gggtgttatcagccaagagaaatttgagaaatctagagcctatagtcttgataaaagccacttc 
                 
               
               
                   
               
               
                 
                   cattttgttcacgagtttgtgacaatagtgacagactctacaattttgtactttggggtattgc 
                 
               
               
                   
               
               
                 
                   cctggttttggaagaaatcaggagattttatgacaatagctggtttcaatgctgagaatgaaat 
                 
               
               
                   
               
               
                 
                   actgcatacccttgccttcttagcagggctgatgatttggtcacagataacagatttgcccttt 
                 
               
               
                   
               
               
                 
                   tctctgtactcaacttttgtgattgaggcccgtcatggttttaataagcaaacaccatggttat 
                 
               
               
                   
               
               
                 
                   tctttagggacatgcttaaaggaattttcctttctgtaataattggtccacctattgtggctgc 
                 
               
               
                   
               
               
                 
                   aatcattgtaatagtacagaaaggaggtccatacttggccatctatctttgggtttttacgttt 
                 
               
               
                   
               
               
                 
                   ggtctttctattgtgatgatgaccctttatccagtactaatagctccactcttcaataagttca 
                 
               
               
                   
               
               
                 
                   ctccacttccagatggtcaactcagggagaaaatcgagaaacttgcttcctccctcaactatcc 
                 
               
               
                   
               
               
                 
                   gttaaagaaactatttgttgtcgatggatccacaagatcaagtcacagcaatgcctatatgtat 
                 
               
               
                   
               
               
                 
                   ggattcttcaagaacaagaggattgtcccttatgacacattaattcaacagtgcaaagacgatg 
                 
               
               
                   
               
               
                 
                   aggaaattgttgctgttattgcccatgagttgggacactggaagctcaaccatactgtgtacac 
                 
               
               
                   
               
               
                 
                   atttgttgctatgcagattcttacacttctacaatttggaggatatacactagtgcgaaattca 
                 
               
               
                   
               
               
                 
                   gctgatctgtatcgaagctttgggtttgatacgcagccagtcctcattgggctcatcatatttc 
                 
               
               
                   
               
               
                 
                   agcatactgtaatcccacttcagcaattggtcagctttggtctgaacctagtcagccgatcatt 
                 
               
               
                   
               
               
                 
                   tgaatttcaggctgatggctttgccaagaagcttggatatgcatctggattacgcggtggtctt 
                 
               
               
                   
               
               
                 
                   gtgaaactacaggaggagaatctgtcagctatgaatacagatccttggtactctgcttatcact 
                 
               
               
                   
               
               
                 
                   attctcatcctccccttgttgaaagattggccgcgctggacgaaccggataagaaggaagacta 
                 
               
               
                   
               
               
                   a gagctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgt 
               
               
                   
               
               
                 tgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaac 
               
               
                   
               
               
                 atgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacattt 
               
               
                   
               
               
                 aatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatc 
               
               
                   
               
               
                 tatgttactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctgg 
               
               
                   
               
               
                 cgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagag 
               
               
                   
               
               
                 gcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttc 
               
               
                   
               
               
                 ttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctt 
               
               
                   
               
               
                 tagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttc 
               
               
                   
               
               
                 acgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttcttt 
               
               
                   
               
               
                 aatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatt 
               
               
                   
               
               
                 tataagggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaacc 
               
               
                   
               
               
                 agcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccg 
               
               
                   
               
               
                 tctcactggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagtt 
               
               
                   
               
               
                 gtctaagcgtcaattt gtttacaccacaatatatcctgcca   
               
            
           
         
       
     
     SEQ ID NO:139 is the nucleic acid sequence of pRD29A-GmCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the RD29A promoter. Sequence in bold is the GmCPP sense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 140 
                   
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaagggtttgattacttctattggaaag gactctaga ccggt   
               
               
                   
               
               
                 
                   tcgtccagcgcggccaatctttcaacaaggggaggatgagaatagtgataagcagagtaccaag 
                 
               
               
                   
               
               
                 
                   gatctgtattcatagctgacagattctcctcctgtagtttcacaagaccaccgcgtaatccaga 
                 
               
               
                   
               
               
                 
                   tgcatatccaagcttcttggcaaagccatcagcctgaaattcaaatgatcggctgactaggttc 
                 
               
               
                   
               
               
                 
                   agaccaaagctgaccaattgctgaagtgggattacagtatgctgaaatatgatgagcccaatga 
                 
               
               
                   
               
               
                 
                   ggactggctgcgtatcaaacccaaagcttcgatacagatcagctgaatttcgcactagtgtata 
                 
               
               
                   
               
               
                 
                   tcctccaaattgtagaagtgtaagaatctgcatagcaacaaatgtgtacacagtatggttgagc 
                 
               
               
                   
               
               
                 
                   ttccagtgtcccaactcatgggcaataacagcaacaatttcctcatcgtctttgcactgttgaa 
                 
               
               
                   
               
               
                 
                   ttaatgtgtcataagggacaatcctcttgttcttgaagaatccatacatataggcattgctgtg 
                 
               
               
                   
               
               
                   acttgatcttgt ggatccccATCTACCCGCTTCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGG 
               
               
                   
               
               
                 CGAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCTTTGGTCGTCATGAAGATGCG 
               
               
                   
               
               
                 GACTTGCGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGACCACGCATTAATGGACTGGA 
               
               
                   
               
               
                 TTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGA 
               
               
                   
               
               
                 TGAACATGGCATCGTGGTGATTGATGAAACTGCTGCTGTCGGCTTTTCGCTCTCTTTAGGCATT 
               
               
                   
               
               
                 GGTTTCGAAGCGGGCAACAAGCCGAAAGAACTGTACAGCGAAGAGGCAGTCAACGGGGAAACTC 
               
               
                   
               
               
                 AGCAAGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGGT 
               
               
                   
               
               
                 GATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCCA 
               
               
                   
               
               
                 CTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCT 
               
               
                   
               
               
                 GCGACGCTCACACCGATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGG 
               
               
                   
               
               
                 ATGGTATGTCCAAAGCGGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGCC 
               
               
                   
               
               
                 TGGCAGGAGAAACTGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGT 
               
               
                   
               
               
                 ATCACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTT 
               
               
                   
               
               
                 TGCGACCTCGCAAGGCATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCGC 
               
               
                   
               
               
                 AAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAAC 
               
               
                   
               
               
                 CGCAGCAGGGAGGCAAACAATGAatcaacaactctcctggcgcaccatcgtcggctacagcctc 
               
               
                   
               
               
                 gggaattgctaccgagctc   acaagatcaagtcacagcaatgcctatatgtatggattcttcaag     
               
               
                   
               
               
                 
                   
                     aacaagaggattgtcccttatgacacattaattcaacagtgcaaagacgatgaggaaattgttg 
                   
                 
               
               
                   
               
               
                 
                   
                     ctgttattgcccatgagttgggacactggaagctcaaccatactgtgtacacatttgttgctat 
                   
                 
               
               
                   
               
               
                 
                   
                     gcagattcttacacttctacaatttggaggatatacactagtgcgaaattcagctgatctgtat 
                   
                 
               
               
                   
               
               
                 
                   
                     cgaagctttgggtttgatacgcagccagtcctcattgggctcatcatatttcagcatactgtaa 
                   
                 
               
               
                   
               
               
                 
                   
                     tcccacttcagcaattggtcagctttggtctgaacctagtcagccgatcatttgaatttcaggc 
                   
                 
               
               
                   
               
               
                 
                   
                     tgatggctttgccaagaagcttggatatgcatctggattacgcggtggtcttgtgaaactacag 
                   
                 
               
               
                   
               
               
                 
                   
                     gaggagaatctgtcagctatgaatacagatccttggtactctgcttatcactattctcatcctc 
                   
                 
               
               
                   
               
               
                     cccttgttgaaagattggccgcgctggacgaaccgg   gagctcgaatttccccgatcgttcaaac 
               
               
                   
               
               
                 atttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataat 
               
               
                   
               
               
                 ttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatg 
               
               
                   
               
               
                 ggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgc 
               
               
                   
               
               
                 gcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcactggcc 
               
               
                   
               
               
                 gtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcac 
               
               
                   
               
               
                 atccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagtt 
               
               
                   
               
               
                 gcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccgg 
               
               
                   
               
               
                 ctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcac 
               
               
                   
               
               
                 ctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacgg 
               
               
                   
               
               
                 tttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaac 
               
               
                   
               
               
                 aacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggaacca 
               
               
                   
               
               
                 ccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctca 
               
               
                   
               
               
                 gggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccacccca 
               
               
                   
               
               
                 gtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttacaccacaa   
               
               
                   
               
               
                 
                   tatatcctgcca 
                 
               
            
           
         
       
     
     SEQ ID NO:140 is the nucleic acid sequence of pRD29A-HP-GmCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the RD29A promoter. Sequence in bold is the GmCPP antisense sequence, bold and underlined sequence is the GmCPP sense sequence. 
     
       
         
           
               
               
               
            
               
                   
                 SEQ ID NO: 141 
                   
               
               
                   
                 
                   
                   
                   aaacactgatagtttaaactgaaggcgggaaacgacaatctgatcatgagcggagaattaagg 
                 
               
               
                   
               
               
                   
                 
                   gagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttggaactgacagaaccgcaacgttgaaggagccac 
                 
               
               
                   
               
               
                   
                 
                   tcagccgcgggtttctggagtttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcactat 
                 
               
               
                   
               
               
                   
                 
                   cagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggtatccaattagagtctcatattcactctcaa 
                 
               
               
                   
               
               
                   
                 
                   tccaaataatctgcaccggatctggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggaga 
                 
               
               
                   
               
               
                   
                 
                   ggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggtt 
                 
               
               
                   
               
               
                   
                 
                   ctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgt 
                 
               
               
                   
               
               
                   
                 
                   tccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtc 
                 
               
               
                   
               
               
                   
                 
                   atctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattc 
                 
               
               
                   
               
               
                   
                 
                   gaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagag 
                 
               
               
                   
               
               
                   
                 
                   catcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcg 
                 
               
               
                   
               
               
                   
                 
                   atgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatca 
                 
               
               
                   
               
               
                   
                 
                   ggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgct 
                 
               
               
                   
               
               
                   
                 
                   cccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
                 
               
               
                   
               
               
                   
                 
                   gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctgg 
                 
               
               
                   
               
               
                   
                 
                   atgatcctccagcgcggggatctcatgctggagttcttcgcccacgggatctctgcggaacaggcggtcgaaggtgccgatatcatt 
                 
               
               
                   
               
               
                   
                 
                   acgacagcaacggccgacaagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtcgg 
                 
               
               
                   
               
               
                   
                 
                   cggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcgtggagttcccgccacagacccgga 
                 
               
               
                   
               
               
                   
                 
                   tgatccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgtt 
                 
               
               
                   
               
               
                   
                 
                   gaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacat 
                 
               
               
                   
               
               
                   
                 
                   ttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggcct 
                 
               
               
                   
               
               
                   
                 
                   cctgtcaatgctggcggcggctctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggc 
                 
               
               
                   
               
               
                   
                 
                   ggctctgagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatg 
                 
               
               
                   
               
               
                   
                 
                   accgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgctgctatcgat 
                 
               
               
                   
               
               
                   
                 
                   ggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcggt 
                 
               
               
                   
               
               
                   
                 
                   gacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatcggttgaatgtcgcccttttgtctttggccc 
                 
               
               
                   
               
               
                   
                 
                   aatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtg 
                 
               
               
                   
               
               
                   
                 
                   agcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaatt 
                 
               
               
                   
               
               
                   
                 
                   gtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag 
                   
                     ggagccatagatgcaa 
                   
                 
               
               
                   
               
               
                   
                 
                   
                     ttcaatcaaactgaaatttctgcaagaatctcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaa 
                   
                 
               
               
                   
               
               
                   
                 
                   
                     cttacgaaatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattattatagaattttactggttaaat 
                   
                 
               
               
                   
               
               
                   
                 
                   
                     taaaaatgaatagaaaaggtgaattaagaggagagaggaggtaaacattttcttctattttttcatattttcaggataaattattgta 
                   
                 
               
               
                   
               
               
                   
                 
                   
                     aaagtttacaagatttccatttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatcttctaccagt 
                   
                 
               
               
                   
               
               
                   
                 
                   
                     agaggaataaacaatatttagctcctttgtaaatacaaattaattttccttcttgacatcattcaattttaattttacgtataaaataaa 
                   
                 
               
               
                   
               
               
                   
                 
                   
                     agatcatacctattagaacgattaaggagaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacg 
                   
                 
               
               
                   
               
               
                   
                 
                   
                     taaacgtaaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacattttaggatggaataaatat 
                   
                 
               
               
                   
               
               
                   
                 
                   
                     cataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaataaaagatatactaccgacatgagttccaaaaagc 
                   
                 
               
               
                   
               
               
                   
                 
                   
                     aaaaaaaaagatcaagccgacacagacacgcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatac 
                   
                 
               
               
                   
               
               
                   
                 
                   
                     gtgtccctttatctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaactagaaaacaatcatc 
                   
                 
               
               
                   
               
               
                   
                     aggaataaagggtttgattacttctattggaaag     gactctagaggatccccggg ttagtcttccttcttatccggttcgtccagcgcggcc 
               
               
                   
               
               
                   
                 aatctttcaacaaggggaggatgagaatagtgataagcagagtaccaaggatctgtattcatagctgacagattctcctcctgtagtttcacaa 
               
               
                   
               
               
                   
                 gaccaccgcgtaatccagatgcatatccaagcftcftggcaaagccatcagcctgaaattcaaatgatcggctgactaggttcagaccaaag 
               
               
                   
               
               
                   
                 ctgaccaattgctgaagtgggattacagtatgctgaaatatgatgagcccaatgaggactggctgcgtatcaaacccaaagcttcgatacag 
               
               
                   
               
               
                   
                 atcagctgaatttcgcactagtgtatatcctccaaattgtagaagtgtaagaatctgcatagcaacaaatgtgtacacagtatggttgagcttcc 
               
               
                   
               
               
                   
                 agtgtcccaactcatgggcaataacagcaacaatttcctcatcgtctttgcactgttgaattaatgtgtcataagggacaatcctcttgttcttgaa 
               
               
                   
               
               
                   
                 gaatccatacatataggcattgctgtgacttgatcttgtggatccatcgacaacaaatagtttctttaacggatagttgagggaggaagcaagtt 
               
               
                   
               
               
                   
                 tctcgattttctccctgagttgaccatctggaagtggagtgaacttattgaagagtggagctattagtactggataaagggtcatcatcacaata 
               
               
                   
               
               
                   
                 gaaagaccaaacgtaaaaacccaaagatagatggccaagtatggacctcctttctgtactattacaatgattgcagccacaataggtggacc 
               
               
                   
               
               
                   
                 aattattacagaaaggaaaattcctttaagcatgtccctaaagaataaccatggtgtttgcttattaaaaccatgacgggcctcaatcacaaaag 
               
               
                   
               
               
                   
                 ttgagtacagagaaaagggcaaatctgttatctgtgaccaaatcatcagccctgctaagaaggcaagggtatgcagtatttcattctcagcatt 
               
               
                   
               
               
                   
                 gaaaccagctattgtcataaaatctcctgatttcttccaaaaccagggcaataccccaaagtacaaaattgtagagtctgtcactattgtcacaa 
               
               
                   
               
               
                   
                 actcgtgaacaaaatggaagtggcttttatcaagactataggctctagatttctcaaatttctcttggctgataacaccctctaaagtctttggaag 
               
               
                   
               
               
                   
                 agtaggaagtttgagggccctatgttgtcgcacatccaagtaagtttcaaaaatgtacattaatatcataaatccgacaacggcttccatgtag 
               
               
                   
               
               
                   
                 ggaaacgccat gagctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgat   
               
               
                   
               
               
                   
                 
                   gattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgatt 
                 
               
               
                   
               
               
                   
                 
                   agagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatc 
                 
               
               
                   
               
               
                   
                 
                   tatgttactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgc 
                 
               
               
                   
               
               
                   
                 
                   agcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcg 
                 
               
               
                   
               
               
                   
                 
                   cccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccg 
                 
               
               
                   
               
               
                   
                 
                   atttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttc 
                 
               
               
                   
               
               
                   
                 
                   gccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttg 
                 
               
               
                   
               
               
                   
                 
                   atttataagggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgca 
                 
               
               
                   
               
               
                   
                 
                   actctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccccagtacattaaaaa 
                 
               
               
                   
               
               
                   
                 
                   cgtccgcaatgtgttattaagttgtctaagcgtcaattt 
                   
                   
                 
               
            
           
         
       
     
     SEQ ID NO:141 is the nucleic acid sequence of pRD29A-antisense-GmCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the RD29A promoter. Sequence in bold is the GmCPP antisense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 142 
                   
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatcc atggcgattcctttcatggaaaccgtcgttggttttatgatagtgatgtacgtttttga   
               
               
                   
               
               
                 
                   gacgtatttggatctgaggcaacatactgctctcaagcttcccactctcccaaagactttggtt 
                 
               
               
                   
               
               
                 
                   ggagtcattagccaagagaagtttgagaaatctcgagcttacagtcttgacaaaagccattttc 
                 
               
               
                   
               
               
                 
                   actttgttcatgagtttgttactatacttatggactctgcgattctgttctttgggatcttgcc 
                 
               
               
                   
               
               
                 
                   ttggttttggaagatatctggcggctttctaccaatggtgggactcgatccagagaatgaaatc 
                 
               
               
                   
               
               
                 
                   ctgcacactctttcattcttggctggtcttatgacatggtcacagatcactgatttgccatttt 
                 
               
               
                   
               
               
                 
                   ctttgtactcaactttcgtgatcgagtctcggcatgggttcaacaaacaaacaatatggatgtt 
                 
               
               
                   
               
               
                 
                   cattagggacatgatcaaaggaatactcctctctgtcatacctgcccctcctatcgttgccgca 
                 
               
               
                   
               
               
                 
                   attattgttatagttcagaaaggaggtccttacctcgccatctatctgtgggcattcatgttta 
                 
               
               
                   
               
               
                 
                   tcctgtctctagtgatgatgactatataccctgttttgattgcacctcttttcaacaagttcac 
                 
               
               
                   
               
               
                 
                   tcctcttcctgatggagacctccgggagaagattgagaaacttgcttcttctctaaagtttcct 
                 
               
               
                   
               
               
                 
                   ctgaagaagctgtttgttgtcgatggatctacaaggtcaagccatagtaatgcttacatgtatg 
                 
               
               
                   
               
               
                 
                   gtttcttcaagaacaaaaggattgttctttatgacacattgattcagcagtgccagaatgagaa 
                 
               
               
                   
               
               
                 
                   tgaaattgtggcggttattgcacacgagctgggacactggaagctgaatcacactacatactcg 
                 
               
               
                   
               
               
                 
                   ttcattgctgttcaaatccttgccttcttgcaatttggaggatacactcttgtcagaaactcca 
                 
               
               
                   
               
               
                 
                   ctgatctcttcaggagttttggttttgatacacaaccagttctcattggtttgatcatatttca 
                 
               
               
                   
               
               
                 
                   gcacactgtaataccacttcaacacctagtaagctttgacctcaaccttgttagtcgagcgttt 
                 
               
               
                   
               
               
                 
                   gagtttcaggctgatgcttttgcagtgaatcttggttatgcaaaggatctacgtcctgccctag 
                 
               
               
                   
               
               
                 
                   tgaagctacaggaagagaacttatcagcgatgaacacagacccattgtactcagcttatcacta 
                 
               
               
                   
               
               
                 
                   ctcacaccctcctcttgtagagaggcttcgagccattgatggagaagacaagaagacagattaa 
                 
               
               
                   
               
               
                 cccctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgtt 
               
               
                   
               
               
                 gccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaaca 
               
               
                   
               
               
                 tgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacattta 
               
               
                   
               
               
                 atacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatct 
               
               
                   
               
               
                 atgttactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggc 
               
               
                   
               
               
                 gttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagagg 
               
               
                   
               
               
                 cccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttct 
               
               
                   
               
               
                 tcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctcccttt 
               
               
                   
               
               
                 agggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttca 
               
               
                   
               
               
                 cgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttcttta 
               
               
                   
               
               
                 atagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgattt 
               
               
                   
               
               
                 ataagggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaacca 
               
               
                   
               
               
                 gcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgt 
               
               
                   
               
               
                 ctcactggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttg 
               
               
                   
               
               
                 tctaagcgtcaattt gtttacaccacaatatatcctgcca   
               
            
           
         
       
     
     SEQ ID NO:142 is the nucleic acid sequence of pBI121-BnCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the 35S promoter. Sequence in bold is the BnCPP antisense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 143 
                   
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctaga c   
               
               
                   
               
               
                 
                   cagtgtcccagctcgtgtgcaataaccgccacaatttcattctcattctggcactgctgaatca 
                 
               
               
                   
               
               
                 
                   atgtgtcataaagaacaatccttttgttcttgaagaaaccatacatgtaagcattactatggct 
                 
               
               
                   
               
               
                 
                   tgaccttgtagatccatcgacaacaaacagcttcttcagaggaaactttagagaagaagcaagt 
                 
               
               
                   
               
               
                 
                   ttctcaatcttctcccggaggtctccatcaggaagaggagtgaacttgttgaaaagaggtgcaa 
                 
               
               
                   
               
               
                 
                   tcaaaacagggtatatagtcatcatcactagagacaggataaacatgaatgcccacagatagat 
                 
               
               
                   
               
               
                 
                   ggcgaggtaaggacctcctttctgaactataacaataattgcggcaacgataggaggggcaggt 
                 
               
               
                   
               
               
                 
                   atgacagagaggagtattcctttgatcatgtccctaatgaacatccatattgtttgtttgttga 
                 
               
               
                   
               
               
                 
                   acccatgccgagactcgatcacgaaagttgagtacaaagaaaatggcaaatcagtgatctgtga 
                 
               
               
                   
               
               
                 
                   ccatgtcataagaccagccaagaatgaaagagtgtgcaggatttcattctctggatcgagtccc 
                 
               
               
                   
               
               
                   accattggtagaa ggatccccATCTACCCGCTTCGCGTCGGCATCCGGTCAGTGGCAGTGAAGG 
               
               
                   
               
               
                 GCGAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCTTTGGTCGTCATGAAGATGC 
               
               
                   
               
               
                 GGACTTGCGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGACCACGCATTAATGGACTGG 
               
               
                   
               
               
                 ATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAG 
               
               
                   
               
               
                 ATGAACATGGCATCGTGGTGATTGATGAAACTGCTGCTGTCGGCTTTTCGCTCTCTTTAGGCAT 
               
               
                   
               
               
                 TGGTTTCGAAGCGGGCAACAAGCCGAAAGAACTGTACAGCGAAGAGGCAGTCAACGGGGAAACT 
               
               
                   
               
               
                 CAGCAAGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGG 
               
               
                   
               
               
                 TGATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCC 
               
               
                   
               
               
                 ACTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTC 
               
               
                   
               
               
                 TGCGACGCTCACACCGATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACG 
               
               
                   
               
               
                 GATGGTATGTCCAAAGCGGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGC 
               
               
                   
               
               
                 CTGGCAGGAGAAACTGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATG 
               
               
                   
               
               
                 TATCACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATT 
               
               
                   
               
               
                 TTGCGACCTCGCAAGGCATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCG 
               
               
                   
               
               
                 CAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAA 
               
               
                   
               
               
                 CCGCAGCAGGGAGGCAAACAATGAatcaacaactctcctggcgcaccatcgtcggctacagcct 
               
               
                   
               
               
                 cgggaattgctaccgagctc   ttctaccaatggtgggactcgatccagagaatgaaatcctgcac     
               
               
                   
               
               
                 
                   
                     actctttcattcttggctggtcttatgacatggtcacagatcactgatttgccattttctttgt 
                   
                 
               
               
                   
               
               
                 
                   
                     actcaactttcgtgatcgagtctcggcatgggttcaacaaacaaacaatatggatgttcattag 
                   
                 
               
               
                   
               
               
                 
                   
                     ggacatgatcaaaggaatactcctctctgtcatacctgcccctcctatcgttgccgcaattatt 
                   
                 
               
               
                   
               
               
                 
                   
                     gttatagttcagaaaggaggtccttacctcgccatctatctgtgggcattcatgtttatcctgt 
                   
                 
               
               
                   
               
               
                 
                   
                     ctctagtgatgatgactatataccctgttttgattgcacctcttttcaacaagttcactcctct 
                   
                 
               
               
                   
               
               
                 
                   
                     tcctgatggagacctccgggagaagattgagaaacttgcttcttctctaaagtttcctctgaag 
                   
                 
               
               
                   
               
               
                 
                   
                     aagctgtttgttgtcgatggatctacaaggtcaagccatagtaatgcttacatgtatggtttct 
                   
                 
               
               
                   
               
               
                 
                   
                     tcaagaacaaaaggattgttctttatgacacattgattcagcagtgccagaatgagaatgaaat 
                   
                 
               
               
                   
               
               
                     tgtggcggttattgcacacgagctgggacactgg   gagctcgaatttccccgatcgttcaaacat 
               
               
                   
               
               
                 ttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataattt 
               
               
                   
               
               
                 ctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatggg 
               
               
                   
               
               
                 tttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgc 
               
               
                   
               
               
                 aaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcactggccgt 
               
               
                   
               
               
                 cgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacat 
               
               
                   
               
               
                 ccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgc 
               
               
                   
               
               
                 gcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggct 
               
               
                   
               
               
                 ttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacct 
               
               
                   
               
               
                 cgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtt 
               
               
                   
               
               
                 tttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaa 
               
               
                   
               
               
                 cactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggaaccacc 
               
               
                   
               
               
                 atcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagg 
               
               
                   
               
               
                 gccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccccagt 
               
               
                   
               
               
                 acattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttacaccacaata   
               
               
                   
               
               
                 
                   tatcctgcca 
                 
               
            
           
         
       
     
     SEQ ID NO:143 is the nucleic acid sequence of pBI121-HP-BnCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the 35S promoter. Sequence in bold is the BnCPP antisense sequence, bold and underlined sequence is the BnCPP sense fragment and upper case indicates the truncated GUS fragment. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 144 
                   
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag cccacagatggttagagaggcttacgcagcaggtctcatcaagacg   
               
               
                   
               
               
                 
                   atctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtca 
                 
               
               
                   
               
               
                 
                   aaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtac 
                 
               
               
                   
               
               
                 
                   tattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtc 
                 
               
               
                   
               
               
                 
                   tctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaa 
                 
               
               
                   
               
               
                 
                   cagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaa 
                 
               
               
                   
               
               
                 
                   gaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagataca 
                 
               
               
                   
               
               
                 
                   gtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcg 
                 
               
               
                   
               
               
                 
                   gattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctccta 
                 
               
               
                   
               
               
                 
                   caaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtccc 
                 
               
               
                   
               
               
                 
                   aaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaa 
                 
               
               
                   
               
               
                 
                   agcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttc 
                 
               
               
                   
               
               
                   gcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggg gactctagag 
               
               
                   
               
               
                 gatcc ttaatctgtcttcttgtcttctccatcaatggctcgaagcctctctacaagaggagggt   
               
               
                   
               
               
                 
                   gtgagtagtgataagctgagtacaatgggtctgtgttcatcgctgataagttctcttcctgtag 
                 
               
               
                   
               
               
                 
                   cttcactagggcaggacgtagatcctttgcataaccaagattcactgcaaaagcatcagcctga 
                 
               
               
                   
               
               
                 
                   aactcaaacgctcgactaacaaggttgaggtcaaagcttactaggtgttgaagtggtattacag 
                 
               
               
                   
               
               
                 
                   tgtgctgaaatatgatcaaaccaatgagaactggttgtgtatcaaaaccaaaactcctgaagag 
                 
               
               
                   
               
               
                 
                   atcagtggagtttctgacaagagtgtatcctccaaattgcaagaaggcaaggatttgaacagca 
                 
               
               
                   
               
               
                 
                   atgaacgagtatgtagtgtgattcagcttccagtgtcccagctcgtgtgcaataaccgccacaa 
                 
               
               
                   
               
               
                 
                   tttcattctcattctggcactgctgaatcaatgtgtcataaagaacaatccttttgttcttgaa 
                 
               
               
                   
               
               
                 
                   gaaaccatacatgtaagcattactatggcttgaccttgtagatccatcgacaacaaacagcttc 
                 
               
               
                   
               
               
                 
                   ttcagaggaaactttagagaagaagcaagtttctcaatcttctcccggaggtctccatcaggaa 
                 
               
               
                   
               
               
                 
                   gaggagtgaacttgttgaaaagaggtgcaatcaaaacagggtatatagtcatcatcactagaga 
                 
               
               
                   
               
               
                 
                   caggataaacatgaatgcccacagatagatggcgaggtaaggacctcctttctgaactataaca 
                 
               
               
                   
               
               
                 
                   ataattgcggcaacgataggaggggcaggtatgacagagaggagtattcctttgatcatgtccc 
                 
               
               
                   
               
               
                 
                   taatgaacatccatattgtttgtttgttgaacccatgccgagactcgatcacgaaagttgagta 
                 
               
               
                   
               
               
                 
                   caaagaaaatggcaaatcagtgatctgtgaccatgtcataagaccagccaagaatgaaagagtg 
                 
               
               
                   
               
               
                 
                   tgcaggatttcattctctggatcgagtcccaccattggtagaaagccgccagatatcttccaaa 
                 
               
               
                   
               
               
                 
                   accaaggcaagatcccaaagaacagaatcgcagagtccataagtatagtaacaaactcatgaac 
                 
               
               
                   
               
               
                 
                   aaagtgaaaatggcttttgtcaagactgtaagctcgagatttctcaaacttctcttggctaatg 
                 
               
               
                   
               
               
                 
                   actccaaccaaagtctttgggagagtgggaagcttgagagcagtatgttgcctcagatccaaat 
                 
               
               
                   
               
               
                 
                   acgtctcaaaaacgtacatcactatcataaaaccaacgacggtttccatgaaaggaatcgccat 
                 
               
               
                   
               
               
                 cccctcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgtt 
               
               
                   
               
               
                 gccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaaca 
               
               
                   
               
               
                 tgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacattta 
               
               
                   
               
               
                 atacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatct 
               
               
                   
               
               
                 atgttactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggc 
               
               
                   
               
               
                 gttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagagg 
               
               
                   
               
               
                 cccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttct 
               
               
                   
               
               
                 tcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctcccttt 
               
               
                   
               
               
                 agggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttca 
               
               
                   
               
               
                 cgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttcttta 
               
               
                   
               
               
                 atagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgattt 
               
               
                   
               
               
                 ataagggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaacca 
               
               
                   
               
               
                 gcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgt 
               
               
                   
               
               
                 ctcactggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttg 
               
               
                   
               
               
                 tctaagcgtcaattt gtttacaccacaatatatcctgcca   
               
            
           
         
       
     
     SEQ ID NO:144 is the nucleic acid sequence of pBI121-antisense-BnCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the 35S promoter. Sequence in bold is the BnCPP antisense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 145 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaaggg tttgattacttctattggaaaggactctagaggatc 
               
               
                   
               
               
                 c atggcgattcctttcatggaaaccgtcgttggttttatgatagtgatgtacgtttttgagacg   
               
               
                   
               
               
                 
                   tatttggatctgaggcaacatactgctctcaagcttcccactctcccaaagactttggttggag 
                 
               
               
                   
               
               
                 
                   tcattagccaagagaagtttgagaaatctcgagcttacagtcttgacaaaagccattttcactt 
                 
               
               
                   
               
               
                 
                   tgttcatgagtttgttactatacttatggactctgcgattctgttctttgggatcttgccttgg 
                 
               
               
                   
               
               
                 
                   ttttggaagatatctggcggctttctaccaatggtgggactcgatccagagaatgaaatcctgc 
                 
               
               
                   
               
               
                 
                   acactctttcattcttggctggtcttatgacatggtcacagatcactgatttgccattttcttt 
                 
               
               
                   
               
               
                 
                   gtactcaactttcgtgatcgagtctcggcatgggttcaacaaacaaacaatatggatgttcatt 
                 
               
               
                   
               
               
                 
                   agggacatgatcaaaggaatactcctctctgtcatacctgcccctcctatcgttgccgcaatta 
                 
               
               
                   
               
               
                 
                   ttgttatagttcagaaaggaggtccttacctcgccatctatctgtgggcattcatgtttatcct 
                 
               
               
                   
               
               
                 
                   gtctctagtgatgatgactatataccctgttttgattgcacctcttttcaacaagttcactcct 
                 
               
               
                   
               
               
                 
                   cttcctgatggagacctccgggagaagattgagaaacttgcttcttctctaaagtttcctctga 
                 
               
               
                   
               
               
                 
                   agaagctgtttgttgtcgatggatctacaaggtcaagccatagtaatgcttacatgtatggttt 
                 
               
               
                   
               
               
                 
                   cttcaagaacaaaaggattgttctttatgacacattgattcagcagtgccagaatgagaatgaa 
                 
               
               
                   
               
               
                 
                   attgtggcggttattgcacacgagctgggacactggaagctgaatcacactacatactcgttca 
                 
               
               
                   
               
               
                 
                   ttgctgttcaaatccttgccttcttgcaatttggaggatacactcttgtcagaaactccactga 
                 
               
               
                   
               
               
                 
                   tctcttcaggagttttggttttgatacacaaccagttctcattggtttgatcatatttcagcac 
                 
               
               
                   
               
               
                 
                   actgtaataccacttcaacacctagtaagctttgacctcaaccttgttagtcgagcgtttgagt 
                 
               
               
                   
               
               
                 
                   ttcaggctgatgcttttgcagtgaatcttggttatgcaaaggatctacgtcctgccctagtgaa 
                 
               
               
                   
               
               
                 
                   gctacaggaagagaacttatcagcgatgaacacagacccattgtactcagcttatcactactca 
                 
               
               
                   
               
               
                   caccctcctcttgtagagaggcttcgagccattgatggagaagacaagaagacagattaa cccc 
               
               
                   
               
               
                 tcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccg 
               
               
                   
               
               
                 gtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgta 
               
               
                   
               
               
                 atgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatac 
               
               
                   
               
               
                 gcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgt 
               
               
                   
               
               
                 tactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtta 
               
               
                   
               
               
                 cccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccg 
               
               
                   
               
               
                 caccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttcttccc 
               
               
                   
               
               
                 ttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttaggg 
               
               
                   
               
               
                 ttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgta 
               
               
                   
               
               
                 gtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatag 
               
               
                   
               
               
                 tggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataa 
               
               
                   
               
               
                 gggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgt 
               
               
                   
               
               
                 ggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctca 
               
               
                   
               
               
                 ctggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttgtcta 
               
               
                   
               
               
                 agcgtcaattt gtttacaccacaatatatcctgcca   
               
            
           
         
       
     
     SEQ ID NO:145 is the nucleic acid sequence of pRD29A-BnCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the RD29A promoter. Sequence in bold is the BnCPP sense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 146 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaagggtttgattacttctattggaaag gactctaga ccagt   
               
               
                   
               
               
                 
                   gtcccagctcgtgtgcaataaccgccacaatttcattctcattctggcactgctgaatcaatgt 
                 
               
               
                   
               
               
                 
                   gtcataaagaacaatccttttgttcttgaagaaaccatacatgtaagcattactatggcttgac 
                 
               
               
                   
               
               
                 
                   cttgtagatccatcgacaacaaacagcttcttcagaggaaactttagagaagaagcaagtttct 
                 
               
               
                   
               
               
                 
                   caatcttctcccggaggtctccatcaggaagaggagtgaacttgttgaaaagaggtgcaatcaa 
                 
               
               
                   
               
               
                 
                   aacagggtatatagtcatcatcactagagacaggataaacatgaatgcccacagatagatggcg 
                 
               
               
                   
               
               
                 
                   aggtaaggacctcctttctgaactataacaataattgcggcaacgataggaggggcaggtatga 
                 
               
               
                   
               
               
                 
                   cagagaggagtattcctttgatcatgtccctaatgaacatccatattgtttgtttgttgaaccc 
                 
               
               
                   
               
               
                 
                   atgccgagactcgatcacgaaagttgagtacaaagaaaatggcaaatcagtgatctgtgaccat 
                 
               
               
                   
               
               
                 
                   gtcataagaccagccaagaatgaaagagtgtgcaggatttcattctctggatcgagtcccacca 
                 
               
               
                   
               
               
                   ttggtagaa ggatccccATCTACCCGCTTCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCGA 
               
               
                   
               
               
                 ACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCTTTGGTCGTCATGAAGATGCGGAC 
               
               
                   
               
               
                 TTGCGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGACCACGCATTAATGGACTGGATTG 
               
               
                   
               
               
                 GGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGA 
               
               
                   
               
               
                 ACATGGCATCGTGGTGATTGATGAAACTGCTGCTGTCGGCTTTTCGCTCTCTTTAGGCATTGGT 
               
               
                   
               
               
                 TTCGAAGCGGGCAACAAGCCGAAAGAACTGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGC 
               
               
                   
               
               
                 AAGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGGTGAT 
               
               
                   
               
               
                 GTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCCACTG 
               
               
                   
               
               
                 GCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCG 
               
               
                   
               
               
                 ACGCTCACACCGATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATG 
               
               
                   
               
               
                 GTATGTCCAAAGCGGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGG 
               
               
                   
               
               
                 CAGGAGAAACTGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATC 
               
               
                   
               
               
                 ACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGC 
               
               
                   
               
               
                 GACCTCGCAAGGCATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCGCAAA 
               
               
                   
               
               
                 CCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGC 
               
               
                   
               
               
                 AGCAGGGAGGCAAACAATGAATCAACAACTCTCCTGGCGCACCATCGTCGGCTACAGCCTCGGG 
               
               
                   
               
               
                 AATTGCTACCGAGCTC   ttctaccaatggtgggactcgatccagagaatgaaatcctgcacactc     
               
               
                   
               
               
                 
                   
                     tttcattcttggctggtcttatgacatggtcacagatcactgatttgccattttctttgtactc 
                   
                 
               
               
                   
               
               
                 
                   
                     aactttcgtgatcgagtctcggcatgggttcaacaaacaaacaatatggatgttcattagggac 
                   
                 
               
               
                   
               
               
                 
                   
                     atgatcaaaggaatactcctctctgtcatacctgcccctcctatcgttgccgcaattattgtta 
                   
                 
               
               
                   
               
               
                 
                   
                     tagttcagaaaggaggtccttacctcgccatctatctgtgggcattcatgtttatcctgtctct 
                   
                 
               
               
                   
               
               
                 
                   
                     agtgatgatgactatataccctgttttgattgcacctcttttcaacaagttcactcctcttcct 
                   
                 
               
               
                   
               
               
                 
                   
                     gatggagacctccgggagaagattgagaaacttgcttcttctctaaagtttcctctgaagaagc 
                   
                 
               
               
                   
               
               
                 
                   
                     tgtttgttgtcgatggatctacaaggtcaagccatagtaatgcttacatgtatggtttcttcaa 
                   
                 
               
               
                   
               
               
                 
                   
                     gaacaaaaggattgttctttatgacacattgattcagcagtgccagaatgagaatgaaattgtg 
                   
                 
               
               
                   
               
               
                     gcggttat   tgcacacgagctgggacactgggagctcgaatttccccgatcgttcaaacatttgg 
               
               
                   
               
               
                 caataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgt 
               
               
                   
               
               
                 tgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttt 
               
               
                   
               
               
                 tatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaac 
               
               
                   
               
               
                 taggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcactggccgtcgtt 
               
               
                   
               
               
                 ttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatcccc 
               
               
                   
               
               
                 ctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcag 
               
               
                   
               
               
                 cctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttcc 
               
               
                   
               
               
                 ccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgac 
               
               
                   
               
               
                 cccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttc 
               
               
                   
               
               
                 gccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacact 
               
               
                   
               
               
                 caaccctatctcgggctattcttttgatttataagggattttgccgatttcggaaccaccatca 
               
               
                   
               
               
                 aacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggcca 
               
               
                   
               
               
                 ggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccccagtacat 
               
               
                   
               
               
                 taaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttacaccacaatatatc   
               
               
                   
               
               
                 
                   ctgcca 
                 
               
            
           
         
       
     
     SEQ ID NO:146 is the nucleic acid sequence of pRD29A-HP-BnCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the RD29A promoter. Sequence in bold is the BnCPP antisense sequence, bold and underlined sequence is BnCPP sense fragment and the upper case sequence represents the truncated GUS fragment. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 147 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagcttgcatgcctgcag ggagccatagatgcaattcaatcaaactgaaatttctgcaagaatc   
               
               
                   
               
               
                 
                   tcaaacacggagatctcaaagtttgaaagaaaatttatttcttcgactcaaaacaaacttacga 
                 
               
               
                   
               
               
                 
                   aatttaggtagaacttatatacattatattgtaattttttgtaacaaaatgtttttattattat 
                 
               
               
                   
               
               
                 
                   tatagaattttactggttaaattaaaaatgaatagaaaaggtgaattaagaggagagaggaggt 
                 
               
               
                   
               
               
                 
                   aaacattttcttctattttttcatattttcaggataaattattgtaaaagtttacaagatttcc 
                 
               
               
                   
               
               
                 
                   atttgactagtgtaaatgaggaatattctctagtaagatcattatttcatctacttcttttatc 
                 
               
               
                   
               
               
                 
                   ttctaccagtagaggaataaacaatatttagctcctttgtaaatacaaattaattttccttctt 
                 
               
               
                   
               
               
                 
                   gacatcattcaattttaattttacgtataaaataaaagatcatacctattagaacgattaagga 
                 
               
               
                   
               
               
                 
                   gaaatacaattcgaatgagaaggatgtgccgtttgttataataaacagccacacgacgtaaacg 
                 
               
               
                   
               
               
                 
                   taaaatgaccacatgatgggccaatagacatggaccgactactaataatagtaagttacatttt 
                 
               
               
                   
               
               
                 
                   aggatggaataaatatcataccgacatcagttttgaaagaaaagggaaaaaaagaaaaaataaa 
                 
               
               
                   
               
               
                 
                   taaaagatatactaccgacatgagttccaaaaagcaaaaaaaaagatcaagccgacacagacac 
                 
               
               
                   
               
               
                 
                   gcgtagagagcaaaatgactttgacgtcacaccacgaaaacagacgcttcatacgtgtcccttt 
                 
               
               
                   
               
               
                 
                   atctctctcagtctctctataaacttagtgagaccctcctctgttttactcacaaatatgcaaa 
                 
               
               
                   
               
               
                   ctagaaaacaatcatcaggaataaaggg tttgattacttctattggaaaggactctagaggatc 
               
               
                   
               
               
                 c ttaatctgtcttcttgtcttctccatcaatggctcgaagcctctctacaagaggagggtgtga   
               
               
                   
               
               
                 
                   gtagtgataagctgagtacaatgggtctgtgttcatcgctgataagttctcttcctgtagcttc 
                 
               
               
                   
               
               
                 
                   actagggcaggacgtagatcctttgcataaccaagattcactgcaaaagcatcagcctgaaact 
                 
               
               
                   
               
               
                 
                   caaacgctcgactaacaaggttgaggtcaaagcttactaggtgttgaagtggtattacagtgtg 
                 
               
               
                   
               
               
                 
                   ctgaaatatgatcaaaccaatgagaactggttgtgtatcaaaaccaaaactcctgaagagatca 
                 
               
               
                   
               
               
                 
                   gtggagtttctgacaagagtgtatcctccaaattgcaagaaggcaaggatttgaacagcaatga 
                 
               
               
                   
               
               
                 
                   acgagtatgtagtgtgattcagcttccagtgtcccagctcgtgtgcaataaccgccacaatttc 
                 
               
               
                   
               
               
                 
                   attctcattctggcactgctgaatcaatgtgtcataaagaacaatccttttgttcttgaagaaa 
                 
               
               
                   
               
               
                 
                   ccatacatgtaagcattactatggcttgaccttgtagatccatcgacaacaaacagcttcttca 
                 
               
               
                   
               
               
                 
                   gaggaaactttagagaagaagcaagtttctcaatcttctcccggaggtctccatcaggaagagg 
                 
               
               
                   
               
               
                 
                   agtgaacttgttgaaaagaggtgcaatcaaaacagggtatatagtcatcatcactagagacagg 
                 
               
               
                   
               
               
                 
                   ataaacatgaatgcccacagatagatggcgaggtaaggacctcctttctgaactataacaataa 
                 
               
               
                   
               
               
                 
                   ttgcggcaacgataggaggggcaggtatgacagagaggagtattcctttgatcatgtccctaat 
                 
               
               
                   
               
               
                 
                   gaacatccatattgtttgtttgttgaacccatgccgagactcgatcacgaaagttgagtacaaa 
                 
               
               
                   
               
               
                 
                   gaaaatggcaaatcagtgatctgtgaccatgtcataagaccagccaagaatgaaagagtgtgca 
                 
               
               
                   
               
               
                 
                   ggatttcattctctggatcgagtcccaccattggtagaaagccgccagatatcttccaaaacca 
                 
               
               
                   
               
               
                 
                   aggcaagatcccaaagaacagaatcgcagagtccataagtatagtaacaaactcatgaacaaag 
                 
               
               
                   
               
               
                 
                   tgaaaatggcttttgtcaagactgtaagctcgagatttctcaaacttctcttggctaatgactc 
                 
               
               
                   
               
               
                 
                   caaccaaagtctttgggagagtgggaagcttgagagcagtatgttgcctcagatccaaatacgt 
                 
               
               
                   
               
               
                   ctcaaaaacgtacatcactatcataaaaccaacgacggtttccatgaaaggaatcgccat cccc 
               
               
                   
               
               
                 tcgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccg 
               
               
                   
               
               
                 gtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgta 
               
               
                   
               
               
                 atgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatac 
               
               
                   
               
               
                 gcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgt 
               
               
                   
               
               
                 tactagatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtta 
               
               
                   
               
               
                 cccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccg 
               
               
                   
               
               
                 caccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttcttccc 
               
               
                   
               
               
                 ttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttaggg 
               
               
                   
               
               
                 ttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgta 
               
               
                   
               
               
                 gtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatag 
               
               
                   
               
               
                 tggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataa 
               
               
                   
               
               
                 gggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgt 
               
               
                   
               
               
                 ggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctca 
               
               
                   
               
               
                 ctggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttgtcta 
               
               
                   
               
               
                 agcgtcaattt gtttacaccacaatatatcctgcca   
               
            
           
         
       
     
     SEQ ID NO:147 is the nucleic acid sequence of pRD29A-antisense-BnCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the RD29A promoter. Sequence in bold is the BnCPP antisense sequence. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 148 
                   
               
            
           
           
               
               
            
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatc 
                   
               
               
                   
               
               
                 tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccg 
               
               
                   
               
               
                 ttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagccgcgggtttctggag 
               
               
                   
               
               
                 tttaatgagctaagcacatacgtcagaaaccattattgcgcgttcaaaagtcgcctaaggtcac 
               
               
                   
               
               
                 tatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaattcccctcggta 
               
               
                   
               
               
                 tccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgc 
               
               
                   
               
               
                 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 
               
               
                   
               
               
                 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 
               
               
                   
               
               
                 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggca 
               
               
                   
               
               
                 gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactg 
               
               
                   
               
               
                 aagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 
               
               
                   
               
               
                 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 
               
               
                   
               
               
                 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 
               
               
                   
               
               
                 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgtt 
               
               
                   
               
               
                 cgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcgatgcctgc 
               
               
                   
               
               
                 ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtg 
               
               
                   
               
               
                 tggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcga 
               
               
                   
               
               
                 atgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttc 
               
               
                   
               
               
                 tatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac 
               
               
                   
               
               
                 gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcgga 
               
               
                   
               
               
                 atcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcg 
               
               
                   
               
               
                 cccacgggatctctgcggaacaggcggtcgaaggtgccgatatcattacgacagcaacggccga 
               
               
                   
               
               
                 caagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatcaacggcgtc 
               
               
                   
               
               
                 ggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcg 
               
               
                   
               
               
                 tggagttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttctt 
               
               
                   
               
               
                 aagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaag 
               
               
                   
               
               
                 catgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcc 
               
               
                   
               
               
                 cgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatc 
               
               
                   
               
               
                 gcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggctctggtgg 
               
               
                   
               
               
                 tggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctct 
               
               
                   
               
               
                 gagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacg 
               
               
                   
               
               
                 ctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaa 
               
               
                   
               
               
                 acttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggc 
               
               
                   
               
               
                 cttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcg 
               
               
                   
               
               
                 gtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatc 
               
               
                   
               
               
                 ggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccg 
               
               
                   
               
               
                 attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                   
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat 
               
               
                   
               
               
                 gttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcc 
               
               
                   
               
               
                 aagct gggaaatttttcgccagttctaaatatccggaaacctcttgggatgccattgcccatct   
               
               
                   
               
               
                 
                   atctgtaatttattgacgaaatagacgaaaaggaaggtggctcctataaagcacatcattgcga 
                 
               
               
                   
               
               
                 
                   taacagaaaggccattgttgaagatacctctgctgacattggtccccaagtggaagcaccaccc 
                 
               
               
                   
               
               
                 
                   catgaggagcaccgtggagtaagaagacgttcgagccacgtcgaaaaagcaagtgtgttgatgt 
                 
               
               
                   
               
               
                 
                   agtatctccattgacgtaagggatgacgcacaatccaactatccatcgcaagaccattgctcta 
                 
               
               
                   
               
               
                   tata agaaagttaatatcatttcgagtggccacgctgagggggatcc atggcgattcctttcat   
               
               
                   
               
               
                 
                   ggaaaccgtcgttggttttatgatagtgatgtacgtttttgagacgtatttggatctgaggcaa 
                 
               
               
                   
               
               
                 
                   catactgctctcaagcttcccactctcccaaagactttggttggagtcattagccaagagaagt 
                 
               
               
                   
               
               
                 
                   ttgagaaatctcgagcttacagtcttgacaaaagccattttcactttgttcatgagtttgttac 
                 
               
               
                   
               
               
                 
                   tatacttatggactctgcgattctgttctttgggatcttgccttggttttggaagatatctggc 
                 
               
               
                   
               
               
                 
                   ggctttctaccaatggtgggactcgatccagagaatgaaatcctgcacactctttcattcttgg 
                 
               
               
                   
               
               
                 
                   ctggtcttatgacatggtcacagatcactgatttgccattttctttgtactcaactttcgtgat 
                 
               
               
                   
               
               
                 
                   cgagtctcggcatgggttcaacaaacaaacaatatggatgttcattagggacatgatcaaagga 
                 
               
               
                   
               
               
                 
                   atactcctctctgtcatacctgcccctcctatcgttgccgcaattattgttatagttcagaaag 
                 
               
               
                   
               
               
                 
                   gaggtccttacctcgccatctatctgtgggcattcatgtttatcctgtctctagtgatgatgac 
                 
               
               
                   
               
               
                 
                   tatataccctgttttgattgcacctcttttcaacaagttcactcctcttcctgatggagacctc 
                 
               
               
                   
               
               
                 
                   cgggagaagattgagaaacttgcttcttctctaaagtttcctctgaagaagctgtttgttgtcg 
                 
               
               
                   
               
               
                 
                   atggatctacaaggtcaagccatagtaatgcttacatgtatggtttcttcaagaacaaaaggat 
                 
               
               
                   
               
               
                 
                   tgttctttatgacacattgattcagcagtgccagaatgagaatgaaattgtggcggttattgca 
                 
               
               
                   
               
               
                 
                   cacgagctgggacactggaagctgaatcacactacatactcgttcattgctgttcaaatccttg 
                 
               
               
                   
               
               
                 
                   ccttcttgcaatttggaggatacactcttgtcagaaactccactgatctcttcaggagttttgg 
                 
               
               
                   
               
               
                 
                   ttttgatacacaaccagttctcattggtttgatcatatttcagcacactgtaataccacttcaa 
                 
               
               
                   
               
               
                 
                   cacctagtaagctttgacctcaaccttgttagtcgagcgtttgagtttcaggctgatgcttttg 
                 
               
               
                   
               
               
                 
                   cagtgaatcttggttatgcaaaggatctacgtcctgccctagtgaagctacaggaagagaactt 
                 
               
               
                   
               
               
                 
                   atcagcgatgaacacagacccattgtactcagcttatcactactcacaccctcctcttgtagag 
                 
               
               
                   
               
               
                   aggcttcgagccattgatggagaagacaagaagacagattaa cccctcgaatttccccgatcgt 
               
               
                   
               
               
                 tcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatca 
               
               
                   
               
               
                 tataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttat 
               
               
                   
               
               
                 gagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaata 
               
               
                   
               
               
                 tagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattca 
               
               
                   
               
               
                 ctggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttg 
               
               
                   
               
               
                 cagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttccca 
               
               
                   
               
               
                 acagttgcgcagcctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgtt 
               
               
                   
               
               
                 cgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgcttta 
               
               
                   
               
               
                 cggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgat 
               
               
                   
               
               
                 agacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaac 
               
               
                   
               
               
                 tggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcg 
               
               
                   
               
               
                 gaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaact 
               
               
                   
               
               
                 ctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaacc 
               
               
                   
               
               
                 accccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtttaca   
               
               
                   
               
               
                 
                   ccacaatatatcctgcca 
                 
               
            
           
         
       
     
     SEQ ID NO:148 is the nucleic acid sequence of MuA-BnCPP. Italicized sequences are the right and left border repeats. Underlined sequence is the MuA promoter. Sequence in bold is the BnCPP sense sequence. 
     Example 33 
     Southern Analysis 
     Genomic Southern blot analysis of transgenic  Arabidopsis  was performed using standard techniques known to one skilled in the art. Typically, 10 g of DNA was electrophoresed in a 0.8% agarose gel and transferred to an appropriate membrane such as Hybond N+ (Amersham Pharmacia Biotech). Pre-hybridization and hybridization conditions were as suggested by the membrane manufacturer, typically at 65° C. The final stringency wash was typically at 1×SSC and 0.1% SDS at 65° C. The NPTII coding region was typically used as the radiolabeled probe in Southern blot analysis. 
     Thirty-seven  Arabidopsis  lines were selected as homozygous pBI121-AtCPP over-expression lines for further examination.  FIG. 27  shows a representative blot confirming the presence of the pBI121-AtCPP transgene. Lines were confirmed to be transgenic by PCR analysis using transgene specific primers in the PCR assays. 
     Thirty-three  Arabidopsis  lines were selected as homozygous pBI121-HP-AtCPP hair-pin down-regulation lines for further examination.  FIG. 28  shows a representative blot confirming the presence of the pBI121-HP-AtCPP hair-pin construct. All lines were confirmed to be transgenic by PCR analysis using transgene specific primers in the PCR assays. 
       Arabidopsis  lines were selected as homozygous pRD29A-AtCPP over-expression lines for further examination.  FIG. 29  shows a representative blot confirming the presence of the pRD29A-AtCPP transgene. Lines were confirmed to be transgenic by PCR analysis using transgene specific primers in the PCR assays. 
       Arabidopsis  lines were selected as homozygous pRD29A-HP-AtCPP lines for further examination.  FIG. 30  shows a representative blot confirming the presence of the pRD29A-HP-AtCPP transgene. Lines were confirmed to be transgenic by PCR analysis using transgene specific primers in the PCR assays. 
     Example 34 
     PCR Analysis of Transgenic Plants 
     PCR was used as a method to confirm the presence of the transgene in all transgenic lines and every construct. Typical PCR mixtures contained: 1× reaction buffer (10 mM Tris-HCl pH 8.8, 1.5 mM MgCl 2 , 50 mM KCl), dNTP&#39;s at 200 μM, 1 pM forward and reverse primer, 2.5 U. Taq DNA polymerase, and template plus water to a final volume of 50 μL. Reactions were run at 1 minute 94° C., 1 minute 60° C., 1 minute 72° C., for 30 cycles. Primers used in the analysis of pBI121-AtCPP and pBI121-HP-AtCPP transgenic plants were as shown in Table 20. Primers used in the analysis of pRD29A-AtCPP were RD29AP1 (SEQ ID NO:161) and SEQ ID NO:102. Primers used in the analysis of pRD29A-HP-AtCPP transgenic plants were those identified as RD29AP1 (SEQ ID NO:161), SEQ ID NO:103 and SEQ ID NO:103, Nosterm-RV (SEQ ID NO:162). 
     
       
         
           
               
               
               
               
             
               
                 TABLE 20 
               
               
                   
               
             
            
               
                 pBI121-AtCPP BamFW: 
                 5′-GCCGACAGTGGTCCCAAAGATGG-3′ 
                 (SEQ ID NO: 105) 
                   
               
               
                   
               
               
                 p35S-AtCPP SmaRV: 
                 5′-AAACCCGGGTTAATCTGTCTTCTTGTCTTCTCCA-3′ 
                 (SEQ ID NO: 102) 
               
               
                   
               
               
                 p35S-HP-AtCPP BamFW: 
                 5′-CTGGAGCTCTTTTACCGAGGTTGGGCCTTGATCC-3′ 
                 (SEQ ID NO: 103) 
               
               
                   
               
               
                 p35S-HP-AtCPP SmaRV: 
                 5′-GCAAGACCGGCAACAGGA-3′ 
                 (SEQ ID NO: 108) 
               
               
                   
               
               
                 pRD29AP1: 
                 5′-TTTAAGCTTGGAGCCATAGATGCAATTCAA-3′ 
                 (SEQ ID NO: 161) 
               
               
                   
               
               
                 pRD29AP1: 
                 5′-TTTAAGCTTGGAGCCATAGATGCAATTCAA-3′ 
                 (SEQ ID NO: 161) 
               
               
                   
               
               
                 Nosterm-RV: 
                 5′-GCAAGACCGGCAACAGGA-3′ 
                 (SEQ ID NO: 162) 
               
               
                   
               
            
           
         
       
     
     Example 35 
     Northern Analysis of Transgenic Plants 
     Total RNA was isolated from developing leaf tissue of 27 35S-AtCPP  Arabidopsis  lines (T3 plants). Approximately 10 μg of total RNA was loaded into each lane. The Northern blot was first probed with P 32  labeled, single-stranded antisense transcript of AtCPP which detects sense transcript, then stripped and re-probed with cDNA of β-tubulin that was used as a reference. The hybridizing bands of AtCPP and β-tubulin were scanned and quantified using the UN-Scan-It programme (Silk Scientific, Utah, USA), and the ratio of the two hybridizing bands for each sample was obtained. The ratio of the wild type plants was set to 100%, and was compared with those of the transgenic lines. Twenty-one out of twenty-seven lines showed higher expression of AtCPP transcript as compared to the wild type. Values ranged from 104% to 282% of wild type. The results of five lines (35, 84, 76, 136, and 156) of the 21 over-expressing lines is shown in  FIG. 31 . 
     Example 36 
     Production of Polyclonal Antibodies Against AtCPP 
     Anti-AtCPP antibodies were generated using AtCPP fusion protein over-expressed in  E. coli . The over-expression vector, pMAL-p2, contains 1175 bp male gene that is located upstream of AtCPP and encodes a 43 KDa maltose-binding protein (MBP). The 1275 bp BamHI/SmaI DNA fragment of AtCPP was inserted into pMAL-p2 at BamHI and SalI sites. The SalI site was converted into blunt end using Klenow fragment. The resulting fusion protein MBP-AtCPP was then over-expressed in DH5α, and purified by one-step affinity for MBP as described by the manufacturer (New England Biolab). The soluble fraction of the crude bacterial extract containing the MBP-AtCPP fusion protein was loaded to a amylose column (1.5 cm×10.0 cm), and the proteins were eluted with 10 mM maltose in column buffer (50 mM Tris-HCl, pH 7.5, 1 mM EDTA, and 200 mM NaCl). Fractions containing purified MBP-AtCPP fusion protein were pooled, and concentrated with a Centriprep-30 concentrator (Amicon). All purification steps were carried out at 4° C. To generate an antibody, the purified fusion protein was further separated by SDS-PAGE and the Coomassie stained band corresponding to the fusion protein was excised. The identity of the fusion protein was confirmed by Western analysis using anti-MBP antibodies (purchased from New England Biolab). The protein was eluted from the gel slice by electroelution and then emulsified in Ribi adjuvant (Ribi Immunochem) to a final volume of 1 ml. MBP-AtCPP protein was injected into a 3 kg New Zealand rabbit on day 1 and booster injections were given on day 21 and day 35 with 175 μg of the protein each time. High-titer antisera were obtained one week after the final injection. 
     Example 37 
     Western Blot Analysis of 35S-AtCPP Transgenic Lines Using Anti-AtCPP Antibodies 
     Western analysis was performed to examine expression level of AtCPP in the transgenic lines compared with that of wild type plants. Anti-Bip antibody, an ER lumenal protein (Stressgen, Victoria, BC, Canada) was used as a reference. Total proteins were extracted from developing leaf tissue of five ABA S  lines and a wild type control. The antigenic protein bands of AtCPP and Bip were scanned and quantified using the UN-Scan-It programme (Silk Scientific, Utah, USA) and the ratio of the two protein bands for each sample was obtained. The ratio of the wild type plants was set to 100%, and was compared with those of the transgenic lines. Data is presented in  FIG. 31  indicating that the AtCPP protein level was increased in the transgenic lines compared to the wild type plants. 
     Example 38 
     ABA Sensitivity of Transgenic Seedlings 
     Approximately 100 seeds were assessed per line per 9 cm plate. Seeds were plated on minimal medium (½ MS) supplemented with no ABA or 1.0 μM ABA. Plates were chilled for 3 days at 4° C. in the dark, and incubated for up to 21 days at 22° C. with 24 hour continuous light. Plates were assessed for germination, cotyledon expansion, true leaf development and seedling vigor. Seedlings were assessed for ABA sensitivity over 21 days of growth at which time sensitive seedlings were arrested at the cotyledon stage, lacked true leaves, and showed inhibition of root growth. Wild type control Columbia plants had two to three pairs of true leaves and a well developed root system. Lines were categorized as ABA sensitive (ABA S ) if less than 1% of plants looked like control, moderately ABA sensitive (ABAMS) if more than 1% but less than 50% of looked like control, or ABA insensitive (ABA Wt ) if greater than 50% looked like control. 
     For example, if a plate had 20 healthy seedlings and the control plate had 60 healthy seedlings, the line would be 33% of control and categorized as moderately ABA sensitive. 
     All four vector constructs (pBI121-AtCPP, pBI121Hp-AtCPP, pRD29AHp-AtCPP, pRD29A-ATCPP) have resulted in transgenic lines of  Arabidopsis  which have increased sensitivity to ABA which is indicative of stress tolerance. The data for all 4 constructs is shown in  FIG. 32 . Of the lines transformed with the pBI121-AtCPP construct to over-express the AtCPP gene, 58% (21 out of 36) were classified as sensitive and an added 30% (11 out of 36) were classified as moderately sensitive. These lines were tested again in T4 and T5 generations and their ABA sensitivity was still present indicating that ABA sensitivity is an inheritable trait. Of the lines transformed with the pBI121-HP-AtCPP construct to down-regulate the AtCPP gene by double stranded RNA-inhibition, 15% (7 out of 45) were classified as sensitive and 31% (14 out of 45) were classified as moderately sensitive. To illustrate the increased sensitivity of transgenic lines to ABA,  FIG. 33  shows the results of germination and seedling development over a range of ABA concentrations. Wild type and pRD29A-HP-AtCPP are compared. Of the lines transformed with pRD29AHp-AtCPP 70% (12 out of 17) showed high sensitivity and 24% (4 out of 17) showed moderate sensitivity to ABA. Of the lines transformed with pRD29A-AtCPP 29% (5 out of 17) showed high sensitivity and 12% (2 out of 17) moderate sensitivity to ABA. Clearly all 4 transgene constructs are altering ABA sensitivity and ABA signal transduction. 
     Example 39 
     Drought Experiments 
       Arabidopsis  plants were grown five plants per 4″ or 3″ pot, in a replicated water-stress experiment. All pots were filled with equal amounts of homogeneous premixed and wetted soil. Plants were grown under 16 hour daylight (150-200 μmol/m 2 /s) at 22° C. and 70% relative humidity. On the day that the first flower opened drought treatment was initiated. First soil water content in each pot was equalized on a weight basis and any further watering of plants was stopped. Daily measurements of soil water content were taken by recording total pot weight. At the end of the drought treatment (6 to 9 days for experiments in 4″ pots and 4-5 days for experiments in 3″ pots) plants were harvested and shoot dry weights determined. Differences in plant growth were factored into the analysis by expressing water loss on a per gram shoot dry weight basis. 
     39a) pBI121-AtCPP, Drought Stress Screen: 
     Analysis of pBI121-AtCPP transgenic lines during water-stress treatment experiments of up to an eight day period, shows a strong trend towards increased soil water content and reduced water loss per gram of shoot biomass. After three days of water-stress treatment most lines had increased soil water content relative to the wild type control with four out of twenty-four lines, 146, 149, 156 and 97, showing a statistically significant difference. The amount of water lost per gram of shoot biomass was lower for all lines except one (95), and thirteen of these lines were significantly different from the wild type Columbia control ( FIG. 34 ). All of the lines showing a statistically significant lower water loss per gram shoot biomass also showed an increased ABA sensitivity. There is also a strong trend, for all but one line (95), which is ABA Wt , towards greater shoot biomass at the end of the drought stress treatment. Seven of those lines 136, 146, 23, 46, 76, 84 and 9, were statistically significant from control at a p=0.05 value. 
     39b) pBI121-AtCPP, Water Loss Per Gram Shoot Biomass During Water Stress Treatment: 
     Lines 35, 76, 95 and a wild type control were grown and placed under a water-stress treatment as above. Plants were harvested at 2 days, 4 days and 6 days of drought treatment. The ABA S  lines, 35 and 76, showed a statistically significant reduction in water-loss relative to shoot dry weight at all three time points (Table 21). Additionally, the two ABA S  transgenic lines had increased shoot biomass, due to increased leaf biomass, and maintained higher soil water contents during drought treatment. 
                     TABLE 21                  Water loss (g) per Shoot dry weight (g) after 2, 4 and 6 days       of drought-stress treatment. Values in bold indicate statistcally       significant differences from Columbia.                                 2 days   4 days   6 days                                         Line   Mean   Std. Error   Mean   Std. Error   Mean   Std. Error                                                 35     212.5     3.5     308.0     9.9     297.7     11.2       76     227.2     5.8     321.2     8.5     293.8     5.0       95   287.0   5.1     377.3     14.8   348.5   25.5       Columbia   265.3   11.8   408.2   7.7   345.9   6.7       Wild type                    
39c) pBI121-AtCPP, Drought Stress and Shoot Recovery:
 
     Water-stress tolerance and determination of post drought-treatment recovery ability was assessed using 20 of the 24 pBI121-AtCPP transgenic lines. Drought treatment was imposed for 6 days after which the plants were watered and allowed to grow for 6 days. Recovered shoot fresh biomass was then determined. Soil water content of these plants was measured daily during the drought treatment and the results confirm previously seen trends. All ABA sensitive (ABA S ) lines that showed a statistically significantly reduction of water loss on a per gram dry weight basis in experiment 39a, continued to show a significant greater soil water content than control plants in this experiment (Table 22). Additionally, Table 22 shows that the recovered shoot fresh biomass after 6 days of drought treatment was significantly greater in all the ABAs lines than Columbia. 
                     TABLE 22                  Soil water content on day 3 of drought treatment and recovered       shoot fresh weight after 6 days of drought treatment       (values in bold were significantly different from Columbia at p = 0.05)                             soil water content               day 3   recovered shoot                                     ABA status   Mean       biomass                                     Line   ABA   (% initial)   Std Error   Mean (g)   Std Error                                             136   ABA S       46.6     1.9     4.5     0.16       14   ABA S       50.25     0.7     4.1     0.12       146   ABA S       45.9     2.5     4.0     0.11       147   ABA S     45.1   1.7     4.0     0.15       149   ABA S     45.3   1.8     3.8     0.17       156   ABA S       47.1     1.9     4.0     0.134       23   ABA S       49     1.4     4.0     0.17       33   ABA S       46.9     1.6     4.3     0.14       35   ABA S     41.7   1.7     4.0     0.11       46   ABA S     44.8   1.7     3.8     0.09       63   ABA S       46.3     1.4     4.0     0.19       76   ABA S       47.8     1.0     3.9     0.17       79   ABA S     45.4   1.1     4.1     0.09       84   ABA S       46.8     1.9     4.1     0.16       85   ABA S     45.3   1.9     4.0     0.12       9   ABA S     45.2   2.1     3.9     0.12       93   ABA wt     43.5   1.2   2.8   0.07       94   ABA S       46.9     1.5     3.9     0.13       97   ABA S       53     1.2     3.8     0.16       95   ABA Wt     41.9   1.2   2.7   0.06       Columbia   ABA Wt     41.3   1.0   2.7   0.04                    
39d) pBI121-AtCPP, Seed Yield after Drought Stress Treatment:
 
     Seed yield after drought stress during flowering was examined using ten pBI121-AtCPP transgenic lines, eight of which were ABA S . Plants were grown one per 4″ pot and were exposed to 9 days of drought treatment as described above. A second group of plants was grown and maintained under well watered conditions as the optimal group. After 9 days of drought treatment plants were re-watered and allowed to continue growth and seed set to maturity. After drought-treatment conditions all eight ABA S  lines had increased yields relative to controls, which ranged from 109% to 126% of the Columbia (Table 23). Drought-treatment resulted in a reduction of yield in all lines, including controls, relative to plants grown under optimal conditions. Expression of the seed yields obtained from drought-treated group relative to the same line under optimal conditions shows that the transgenics preserve a larger percentage of optimal seed yield than do wild type lines. 
                     TABLE 23                  Seed Yield following 9 days drought-treatment                                 ABA   Seed Yield               status   (g per plant)                                         Line   ABA   Mean (g)   Std Error   % Columbia   % Optimal                                             156   ABA S     0.735   0.044   126.2   83.7       63   ABA S     0.675   0.061   116.0   71.0       146   ABA S     0.666   0.053   114.4   72.9       94   ABA S     0.644   0.052   110.6   68.8       84   ABA S     0.642   0.049   110.4   61.8       76   ABA S     0.631   0.055   108.5   66.6       136   ABA S     0.630   0.051   108.3   74.1       35   ABA S     0.614   0.054   105.6   74.2       93   ABA Wt     0.567   0.041   97.5   60.0       95   ABA Wt     0.388   0.088   66.7   43.4       Columbia   ABA Wt     0.582   0.060   100   53.8                    
39e) pBI121-AtCPP, Seed Yield and Growth Under Optimal Water Conditions:
 
     The lines evaluated above and a number of additional lines were examined in a growth and yield experiment under optimal, well-watered conditions. Results indicated that the ABA S  lines were shorter at the stage of first open flower, had more rosette leaves, however, by maturity there were no differences in plant height of transgenics and Columbia. Moreover, the ABA S  transgenics showed similar or higher seed yields ranging from 95% to 121% of the wild type control ( FIG. 35 ). 
     39g) pRD29A-HP-AtCPP Screen for Drought Tolerant Phenotype: 
     Analysis of 17 transgenic lines identified 7 candidate drought tolerant lines (12, 22, 23, 47, 82, 83, 90) on the basis of higher soil water content and lower water loss per g of shoot dry weight (Table 24). All 7 drought tolerant candidate lines showed strong ABA sensitivity and lines that did not show drought tolerance did not show ABA sensitivity. 
     
       
         
           
               
             
               
                 TABLE 24 
               
             
            
               
                   
               
               
                 Soil water content after 3 days of drought treatment and water lost 
               
               
                 per g shoot dry weight. Values in bold are statistically different 
               
               
                 from those of Columbia wild type (p = 0.05) 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 soil water content 
                   
               
               
                   
                 ABA 
                 day 2 
                 water lost in 2days/g 
               
            
           
           
               
               
               
               
               
            
               
                   
                 status 
                 Mean 
                   
                 shootDW 
               
            
           
           
               
               
               
               
               
               
            
               
                 Line 
                 ABA 
                 (% initial) 
                 Std Error 
                 Mean (g/g) 
                 Std Error 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 10 
                 ABA S   
                 33.4 
                 1.6 
                 199.1 
                 4.5 
               
               
                 11 
                 ABA S   
                 34.6 
                 3.3 
                 
                   173.1 
                 
                 1.6 
               
               
                 12 
                 ABA S   
                 36.2 
                 2.0 
                 179.5 
                 5.0 
               
               
                 126 
                 ABA MS   
                 32.5 
                 2.6 
                 199.1 
                 4.1 
               
               
                 127 
                 ABA MS   
                 33.5 
                 2.0 
                 195.6 
                 10.6 
               
               
                 14 
                 ABA S   
                 32.7 
                 1.2 
                 203 
                 4.9 
               
               
                 17 
                 ABA S   
                 29.9 
                 1.8 
                 200.7 
                 7.3 
               
               
                 22 
                 ABA S   
                 
                   39.3 
                 
                 2.1 
                 
                   170.0 
                 
                 3.0 
               
               
                 23 
                 ABA S   
                 35.7 
                 1.4 
                 
                   174.9 
                 
                 2.6 
               
               
                 42 
                 ABA MS   
                 28 
                 0.7 
                 185.4 
                 5.8 
               
               
                 47 
                 ABA S   
                 35.9 
                 2.2 
                 181.2 
                 7.7 
               
               
                 7 
                 ABA Wt   
                 35 
                 1.3 
                 201.8 
                 5.1 
               
               
                 82 
                 ABA S   
                 36.7 
                 2.2 
                 178.3 
                 4.0 
               
               
                 83 
                 ABA S   
                 
                   40 
                 
                 1.4 
                 180.7 
                 6.9 
               
               
                 9 
                 ABA S   
                 31.4 
                 1.4 
                 173.8 
                 8.7 
               
               
                 90 
                 ABA S   
                 
                   38.2 
                 
                 1.3 
                 
                   177.6 
                 
                 6.2 
               
               
                 93 
                 ABA Wt   
                 30.7 
                 1.8 
                 175.3 
                 4.6 
               
               
                 Columbia 
                 ABA Wt   
                 32.1 
                 1.2 
                 196.9 
                 6.2 
               
               
                   
               
            
           
         
       
     
     Example 40 
     Growth Analysis 
     The growth analysis of most promising constructs has been set up at 3 stages. Eight plants per line were grown in 3″ pots with one plant per pot at 22 C, 16 hr light (150-200 μmol/m 2 /s) and 70% RH. Plants were harvested at vegetative growth stage (2 week old seedlings), bolting growth stage (at first open flower) and mid-flowering growth stage (5 to 7 days from first open flower). Also, in some growth experiments additional group of plants was grown in 4″ pots (one per pot and 10 plants per line) to maturity for seed yield determinations. 
     40a) pBI121-AtCPP Growth Under Optimal and Biotic Stress Conditions 
     The growth and productivity of pBI121-AtCPP transgenic  Arabidopsis  lines was examined at several stages of development under optimal growth conditions. Although optimal growth conditions were maintained, plants were assessed to be under a degree of stress that was later determined to be a result of the soil properties. Soil analysis found a fungal contaminant that was believed to be responsible for the biotic stress. This stress could be negated by sterilization of the soil prior to use. Eight ABA S  lines, two with normal ABA sensitivity (ABA Wt ) and a wild type Columbia control were analyzed. 
       FIG. 36  presents the results of various growth (from mid-flowering stage) and yield parameters and each trait is expressed as a percentage of the Columbia control. The results strongly support an enhanced growth phenotype. This enhanced growth phenotype is present at all growth stages. At the vegetative stage, all ABA S  transgenic plants showed an increase in leaf number relative to that of the wild type with four of the eight lines showing a statistically significant difference. The two ABA Wt  lines showed the same or fewer leaves relative to wild type. 
     At the bolting stage ABA S  transgenics showed an increase in leaf number but plants were shorter at this stage (first open flower) than controls. The shoot fresh weight of transgenics was significantly increased relative to that of controls, ranging from 80% to 342% of the wild type. The ABA S  transgenics displayed a delay in flowering from one to three days. The ABA Wt  transgenics did not show delayed flowering, increased shoot fresh weight or increased height. 
     At the flowering stage of development the enhanced growth phenotype is maintained (greater leaf number and fresh weight), however, there were no observable differences in plant height indicating that transgenics bolt shorter but reach same final plant height. 
     Of particular significance is the observation, that under these conditions (biotic stress due to presence of fungi in the soil) yields of the ABA S  transgenics were significantly higher, ranging from 120% to 229% of the wild type control. The ABA Wt  lines showed similar or slightly reduced yields relative to the Columbia control. This finding indicates that ABA S  transgenic lines are affected less by the biotic stress. This observation has been confirmed, where 5 of the drought tolerant lines were grown in contaminated soil to maturity. The seed yields of transgenic lines, even though greatly reduced relative to optimal conditions, were 2.5 to 4.5 fold higher than those of Columbia wild type (Table 25). 
                     TABLE 25                  Seed yield of pBI121-AtCPP lines grown in contaminated soil.       Values in bold indicate statistical differences at p = 0.05                                 ABA   Seed Yield per plant   %       Line   sensitivity   (g)   of Columbia                                       156     ABA S       0.33 ± 0.04     316%         23     ABA S       0.35 ± 0.05     336%         76     ABA S       0.31 ± 0.04     296%         84     ABA S       0.25 ± 0.33     237%         9     ABA S       0.48 ± 0.05     455%         Columbia     ABA Wt     0.11 ± 0.03                        
40b) pBI121-AtCPP Early Seedling Growth:
 
     Four ABA S  and one ABA Wt  line plus Columbia were examined for early seedling growth on agar plates. Twenty seeds were plated in a line on agar plates containing 50% MS with 1% sucrose and vitamins and 6 plates per line were used. Plates were placed on slants, which allowed roots to grow downwards. Root length was measured on 7-day old seedlings and shoot and root biomass determined on 11-day old seedlings. Two of the ABA S  transgenic lines had significantly longer roots and all 4 ABA S  lines had shoot dry weights 114% to 123% of controls and root dry weights of 116% to 151% of controls. As a result, the shoot biomass to rootbiomass ratios were slightly reduced in transgenics. These results indicate that enhanced growth of these transgenics is evident in the early growth stage, shortly after germination, and the root growth is more enhanced relative to shoot growth. In a different experiment seedlings were pulled out of agar and roots were stained with toluidine blue to show their structure.  FIG. 13  shows that transgenic lines had more extensive lateral root system, which would account for greater root biomass. 
     40c) pRD29A-HP-AtCPP Optimal Growth Characteristics 
     An optimal growth study has been conducted with 10 lines as described before. Vegetative growth data showed that two of the lines (12 and 9) had significantly more leaves and seven of the lines (12, 22, 23, 47, 82, 9) had significantly greater shoot biomass. Bolting data showed that eight of the lines (12, 22, 23, 47, 82, 9, 90, 93) were significantly delayed in flowering by one to two days, and seven of the lines were significantly shorter than Columbia at first open flower. All of the lines except 42 and 7 had significantly greater number of rosette leaves and shoot FW and this trend is maintained into the mid-flowering harvest ( FIG. 38 ). The plant height, however, by mid-flowering harvest was not significantly different between the transgenic lines and control. All the lines that showed this enhanced growth also showed drought tolerance and ABA sensitivity. 
     Example 41 
     Ultrastructure pBI121-AtCPP 
     Two of the drought tolerant and ABA S  lines (35 and 76) plus Wt Columbia were used to examine stem and root cross-sections for any differences in ultrastructure. Free hand sections of mature stems (plants flowering for 10 days) were obtained from above the first node, stained with toluidine blue and preserved with glycerol. The stems of transgenic plants appeared to have more dense cellular structure and contain one or two more vascular bundles than those of Columbia Wt indicating more enhanced water and nutrient transport system. 
     Leaf disks were taken and fresh weights determined. Transgenic leaf disks were significantly heavier, 20-24% greater than corresponding wild type controls. This increase is believed to be as a result of a thicker leaf. 
     Example 42 
     Cold Stress Experiment pBI121-AtCPP 
     Four drought tolerant, ABA S  lines (156, 23, 35, 76) and one ABA Wt (95) line plus wild type Columbia were included in a cold stress study. Plants were grown in 3″ pots one per pot) with 10 replicate pots per line at 22 C for 10 days (7 days on agar plates and 4 in soil). The cold stress group was moved into 7° C. for 5 days while the optimal group was left at 22 C. After 5 days in the cold both cold stress group and the optimal group were harvested for shoot biomass determination. ABA S  and drought tolerant lines had significantly greater shoot biomass than Columbia in both optimal (25 to 39% greater shoot fresh weight) and cold stress groups (18 to 44% greater shoot DW) (Table 26). Results of an eight-day cold stress showed that differences between the transgenic lines and Columbia were even more pronounced (53 to 61% greater shoot fresh weight). This result indicates greater plant vigor and better ability of transgenics to cope with cold stress. 
     
       
         
           
               
             
               
                 TABLE 26 
               
             
            
               
                   
               
               
                 Shoot fresh weight of optimal and cold stressed 
               
               
                 (5C for 5d) pBI121-AtCPP. 
               
               
                 Values in bold indicate statistical difference at p = 0.05 
               
            
           
           
               
               
            
               
                   
                 Cold stress 
               
               
                   
                 shoot FW 
               
            
           
           
               
               
               
               
               
            
               
                   
                 ABA 
                 Optimal shoot FW 
                   
                 % of 
               
            
           
           
               
               
               
               
               
               
            
               
                 Line 
                 sensitivity 
                 mg 
                 % of Columbia 
                 mg 
                 Columbia 
               
               
                   
               
               
                 156  
                 ABA S   
                 
                   95.4 ± 3.7 
                 
                 137% 
                 
                   23.1 0.7 
                 
                 118% 
               
               
                 23 
                 ABA S   
                 
                   96.3 ± 3.9 
                 
                 139% 
                 
                   28.3 1.5 
                 
                 144% 
               
               
                 35 
                 ABA S   
                 
                   87.0 ± 1.7 
                 
                 125% 
                 
                   25.3 1.4 
                 
                 130% 
               
               
                 76 
                 ABA S   
                 
                   94.7 ± 2.2 
                 
                 136% 
                 
                   27.3 1.5 
                 
                 140% 
               
               
                 95 
                 ABAWt 
                   67 ± 2.4 
                 96% 
                 21.4 1.0 
                 109% 
               
               
                 Columbia 
                 ABAWt 
                   69 ± 1.9 
                   
                 19.6 1.1 
                   
               
               
                   
               
            
           
         
       
     
     Example 43 
     Drought Stress Under High Temperature pBI121-AtCPP 
     A drought stress experiment was conducted as described above except that day temperature of 32° C. (16 hr) and night temperature of 22° C. (8 hr) was maintained. These temperatures were achieved daily over a 2 hr ramping period. Four ABA S  and one ABA Wt  line plus Columbia were included. Plants were monitored daily for water loss and soil water content and after 5 days of drought treatment half of the plants were harvested and the other half was re-watered and allowed to recover for four days. Shoots were harvested and shoot fresh weight determined. The results (Table 27) of this experiment showed that previously identified drought tolerant lines maintained their drought tolerant phenotype at high temperature and were able to recover well from the drought stress at high temperature 
     
       
         
           
               
             
               
                 TABLE 27 
               
             
            
               
                   
               
               
                 Soil water content on day 2 and water lost in 2 days/final shoot dry 
               
               
                 weight plus recovery shoot FW after 5days of drought stress at 
               
               
                 32 C. day and 22 C. night temperatures. 
               
               
                 Values in bold indicate significant differences from the Columbia control. 
               
            
           
           
               
               
               
               
               
            
               
                   
                 ABA 
                 soil water 
                 water lost in 
                 recovered shoot 
               
               
                 line 
                 sensitivity 
                 content day 2 
                 2 d/shoot DW 
                 FW (g) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 136 
                 ABA S   
                 50.4 ± 1.1 
                   485.7  ± 18.5 
                   1.30  ± 0.04 
               
               
                 146 
                 ABA S   
                   52.1  ± 1.0 
                   504.5  ± 7.9  
                   1.15  ± 0.04 
               
               
                 35 
                 ABA S   
                   52.2  ± 0.8 
                   502.8  ± 15.8 
                   1.19  ± 0.02 
               
               
                 76 
                 ABA S   
                   52.1  ± 0.6 
                   435.6  ± 10.5 
                   1.11  ± 0.03 
               
               
                 95 
                 ABAWt 
                 50.0 ± 0.9 
                 518.2 ± 13.0 
                 0.86 ± 0.03 
               
               
                 Columbia 
                 ABAWt 
                 48.6 ± 0.6 
                 559.7 ± 19.0 
                 0.84 ± 0.03 
               
               
                   
               
            
           
         
       
     
     Example 44 
     Heat Stress and Seed Yield pBI121-AtCPP 
     Two ABA S  lines and one ABA Wt  line plus Columbia were examined for the effect of heat stress during flowering on the final seed yield. Plants were grown in 4 inch pots (one/pot) as described above and 9 days from first open flower the temperature was ramped from 22 C to 43 C over 2 hours and plants were kept at 43 C for 2 hr. Temperature was then ramped back to 22 C over 2 hours and plants were grown under optimal conditions until maturity. The seed yields from this experiment are shown in Table 28. One of the drought tolerant lines (35) had significantly greater yield than Columbia. 
     
       
         
           
               
             
               
                 TABLE 28 
               
             
            
               
                   
               
               
                 Seed yield of pBI121-AtCPP lines after two hour 43 C. heat stress 
               
               
                 9 days from first open flower. 
               
               
                 Values in bold are statistically significant from Columbia. 
               
            
           
           
               
               
               
               
            
               
                   
                 ABA 
                   
                   
               
               
                 Line 
                 sensitivity 
                 seed yield (g/plant) 
                 seed yield (% of col.) 
               
               
                   
               
               
                 35 
                 ABA S   
                   0.55  ± 0.05 
                 347% 
               
               
                 76 
                 ABA S   
                 0.24 ± 0.03 
                 148% 
               
               
                 95 
                 ABAWt 
                 0.11 ± 0.02 
                 69% 
               
               
                 Columbia 
                 ABAWt 
                 0.16 ± 0.03 
                   
               
               
                   
               
            
           
         
       
     
     The effect of heat shock on lines of pBI121-AtCPP at the early flowering stage was assessed. Three ABA S  lines (76, 136, 97) a ABA Wt  line (95) and a Columbia wild type control were seeded in 128 cell flats, one flat per line. At the early flowering stage flats were exposed to a temperature of 46.8° C. for 50 minutes and then returned to normal growth conditions. Lack of continued growth from main meristems was defined as main meristem death and scored for each line. Data is shown in Table 29. 
     
       
         
           
               
             
               
                 TABLE 29 
               
             
            
               
                   
               
               
                 Meristem death due to heat shock 
               
            
           
           
               
               
            
               
                   
                 Line 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Wt 
                 95 
                 76 
                 136 
                 97 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 % Death 
                 91 
                 97 
                 79 
                 59 
                 18 
               
               
                   
               
            
           
         
       
     
     Example 45 
     Stomata Density Determinations pBI121AtCPP 
     Two ABA S  lines (76 and 35) plus Columbia were examined for stomata density on the upper and lower leaf surface. Nail polish imprints of the upper and lower epidermis were obtained from a fully expanded leaf #5. These imprints were analyzed under the microscope and the number of stomata per 8.7×10 −8  m 2  were counted. There were no significant differences found between transgenics and Columbia in the stomata of the upper or lower epidermis (Table 30). The increases seen in drought tolerance and reduced water loss is not attributable to a reduced number of leaf stomata. 
     
       
         
           
               
             
               
                 TABLE 30 
               
             
            
               
                   
               
               
                 Stomata numbers per 8.7 × 10 −8  m 2  of abaxial and adaxial epidermis 
               
               
                 of fully expanded leaf #5 in pBI121AtCPP. 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 stomata on upper 
                 stomata on lower 
               
               
                 Line 
                 ABA sensitivity 
                 epidermis 
                 epidermis 
               
               
                   
               
               
                 35 
                 ABA S   
                 68 ± 5 
                 103 ± 7  
               
               
                 76 
                 ABA S   
                 58 ± 6 
                 120 ± 16 
               
               
                 Columbia 
                 ABAWt 
                 57 ± 6 
                 116 ± 11 
               
               
                   
               
            
           
         
       
     
     Example 46 
     CPP Consensus Sequences 
     Also included in the invention is the CPP consensus sequences. The consensus sequences were generated by alignment of the CPP polypeptide and nucleic acid sequences as well as sequences homologous using the program BioEdit. 
     The “x” in the consensus sequence represents any amino acid or nucleotide. Preferably “x” a conservative amino acid or nucleotide substitution. More preferably, “x” is the most amino acid or nucleotide most prevalent at a given position. For example, the amino acid at position 145 of SEQ ID NO: 168 is a proline as it occurs 66% of the time. 
     
       
         
           
               
             
               
                 TABLE 31 
               
               
                   
               
               
                 ClustalW Analysis of BASF Nucleic Acids 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) BASF_AT1 (SEQ ID NO: 116) 
               
               
                 2) BASF_AT2 (SEQ ID NO: 118) 
               
               
                 3) BASF-Corn (SEQ ID NO: 120) 
               
               
                 4) BASF-Soy (SEQ ID NO: 122) 
               
               
                 5) Consensus (SEQ ID NO: 163) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 32 
               
               
                   
               
               
                 ClustalW Analysis of BASF Amino Acids 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) BASF_AT1 (SEQ ID NO: 117) 
               
               
                 2) BASF_AT2 (SEQ ID NO: 119) 
               
               
                 3) BASF-Corn (SEQ ID NO: 121) 
               
               
                 4) BASF-Soy (SEQ ID NO: 123) 
               
               
                 5) Consensus (SEQ ID NO: 164) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 33 
               
               
                   
               
               
                 ClustalW Analysis of Generic Nucleic Acids 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) afc1      (SEQ ID NO: 124) 
               
               
                 2) AT4g01320 (SEQ ID NO: 126) 
               
               
                 3) AF007269  (SEQ ID NO: 128) 
               
               
                 4) Consensus (SEQ ID NO: 165) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 34 
               
               
                   
               
               
                 ClustalW Analysis of Generic Amino Acids 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) afc1      (SEQ ID NO: 125) 
               
               
                 2) AT4g01320 (SEQ ID NO: 127) 
               
               
                 3) AF007269  (SEQ ID NO: 129) 
               
               
                 4) Consensus (SEQ ID NO: 166) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 35 
               
               
                   
               
               
                 ClustalW Analysis of PPI Nucleic Acids 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) PPI-AtCPP  (SEQ ID NO: 97) 
               
               
                 2) PPI-BnCPP  (SEQ ID NO: 109) 
               
               
                 3) PPI-SoyCPP (SEQ ID NO: 112) 
               
               
                 4) Consensus  (SEQ ID NO: 167) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 36 
               
               
                   
               
               
                 ClustalW Analysis of PPI Amino Acids 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) PPI-AtCPP  (SEQ ID NO: 98) 
               
               
                 2) PPI-BnCPP  (SEQ ID NO: 110) 
               
               
                 3) PPI-SoyCPP (SEQ ID NO: 113) 
               
               
                 4) Consensus  (SEQ ID NO: 168) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 37 
               
               
                   
               
               
                 ClustalW Analysis of PPI/Generic Nucleic Acids 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) PPI-AtCPP  (SEQ ID NO: 97) 
               
               
                 2) PPI-BnCPP  (SEQ ID NO: 109) 
               
               
                 3) PPI-SoyCPP (SEQ ID NO: 112) 
               
               
                 4) afc1       (SEQ ID NO: 124) 
               
               
                 5) AT4g01320  (SEQ ID NO: 126) 
               
               
                 6) AF007269   (SEQ ID NO: 128) 
               
               
                 6) Consensus  (SEQ ID NO: 170) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XX 
               
               
                   
               
               
                 ClustalW Analysis of PPI/Generic Nucleic Acids 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) PPI-AtCPP (SEQ ID NO: 97) 
               
               
                 2) PPI-BnCPP (SEQ ID NO: 109) 
               
               
                 3) PPI-SoyCPP (SEQ ID NO: 112)  
               
               
                 4) afc1 (SEQ ID NO: 124) 
               
               
                 5) AT4g01320 (SEQ ID NO: 126) 
               
               
                 6) AF007269 (SEQ ID NO: 128) 
               
               
                 6) Consensus (SEQ ID NO: 170) 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 38 
               
               
                   
               
               
                 ClustalW Analysis of PPI/Generic Amino Acids 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 1) PPI-AtCPP       (SEQ ID NO: 98) 
               
               
                 2) PPI-BnCPP       (SEQ ID NO: 110) 
               
               
                 3) PPI-SoyCPP      (SEQ ID NO: 113) 
               
               
                 4) afc1            (SEQ ID NO: 125) 
               
               
                 5) AT4g01320       (SEQ ID NO: 127) 
               
               
                 6) AF007269        (SEQ ID NO: 129) 
               
               
                 7) Consensus Gener (SEQ ID NO: 169) 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Example 47 
     Cloning, Vector Construction and Over-Expression of AtFT-B Sequences in  Arabidopsis  Produces a Dominant-Negative Phenotype 
     Farnesyltransferase is a heterodimer formed by its α- and β-subunits and its activity relies on the proper dimerization between these subunits. Increased ABA sensitivity can be achieved by the over-expression of a non-full-length form of AtFTB (SEQ ID NO:1) in  Arabidopsis . In the corollary experiment, over-expression of the full-length AtFTB failed to alter the ABA sensitivity. These results suggest that the phenotype of enhanced ABA response is likely the result of dominant-negative effect of the truncated form AtFTB. The truncated AtFTB maybe nonfunctional or possess limited functionality in vivo as compared to a full length endogenous subunit. However. The reduction of Ft activity results in enhanced ABA sensitivity. 
     Cloning 
     The farnesyltransferase sequence described by SEQ ID NO:1 was cloned into an appropriate vector under the transcriptional control of the 35S CaMV promoter (pBI121 derived vector) in the sense orientation for expression in plant cells. This vector was designated ΔN90AtFTB and designated SEQ ID NO:79. The protein encoded by SEQ ID NO:1 has been determined to lack the 5′ 270 nucleotides, and therefore does not code for the 5′ terminal 90 amino acids. The full length farnesyltransferase sequence was obtained using the primer pair identified by SEQ ID NO:86 and SEQ ID NO:171 and methodology as described elsewhere in this document. The resulting sequence, identified as SEQ ID NO:172 was cloned into an appropriate vector under the transcriptional control of the 35S CaMV promoter (pBI121 derived vector) in the sense orientation for expression in plant cells. This vector was designated pBI121-AtFTB, SEQ ID NO:173. The protein encoded by SEQ ID NO:172 has been determined to represent the full length polypeptide. 
       Agrobacterium -Mediated Transformation, Transgenic Line Selection and ABA Test. 
       Agrobacterium  strain GV3101 carrying the binary constructs described above were transformed into  Arabidopsis thaliana  via  agrobacterium -mediated floral dipping transformation. Transformed  Arabidopsis  lines (T1) were selected on Murashige/Skoog (Sigma) plates containing kanamycin (50 μg/μl). Kanamycin-resistant seedlings were then transferred to soil. The subsequent T2 seeds were harvested from individual transgenic lines for ABA tests. 
     Northern Blot Analysis. 
     Total RNA was isolated from two-week-old T2  Arabidopsis  plants of the pBI121-ΔN90AtFTB, as well as from wild-type Columbia and era1 mutant plants. After separated in the agarose gel, RNA was transferred onto the nitrocellulose membrane and was hybridized with the  32 P-labelled ΔN90AtFTB DNA probe. 
     Over-Expression of pBI121-ΔN90AtFTB, not pBI121-AtFTB Resulted in Enhanced ABA Sensitivity: 
     Transgenic plants were selected and advanced to the second generation. T2 seeds of these two constructs were subjected to ABA test using 0.0, 0.25, 0.5 and 1.0 μM ABA in minimum MS-agarose plates. Of the fifteen pBI121-ΔN90AtFTB lines ten showed an enhanced ABA sensitivity phenotype. At 0.5 μM ABA, the seeds would germinate, however, the development of the seedlings for these 10 lines were retarded or arrested, showing a typical ABA hypersensitive response. In contrast, of the fifteen pBI121-AtFTB transgenic lines, all but one line showed normal wild-type like ABA response to seed germination and seedling development. 
     Northern blot analysis indicated that in the transgenic lines of pBI121-ΔN90AtFTB, the expression levels were higher than the endogenous AtFTB transcript level as depicted by the wild-type control. This indicates the ABA hypersensitive phenotype of these transgenic lines is unlikely due to transcriptional co-suppression. The enhanced ABA response correlates with the results of other methods of AtFTB down-regulation, such as anti-sense and RNAi, hairpin constructs. It is possible that the observed ABA hypersensitive response in ΔN90AtFTB transgenic lines are due to a dominant negative effect. The high transcript levels of ΔN90AtFTB should produce an abundance of the truncated form of AtFTB which may bind to the endogenous AtFTA and result in competitive inhibition of AtFTase activity. 
     Further support for the interaction of truncated FT-B with endogenous FT-A comes from a yeast two-hybrid interaction experiment. Use of the ΔN90AtFTB cDNA as bait, identified interacting clones the majority of which were found to encode FT-A. 
     
       
         
           
               
               
            
               
                 SEQ ID NO: 79 pBI121-ΔN90AtFTB Truncated FT-B Vector 
                   
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatctgatcatgagcgg 
               
               
                   
               
               
                 agaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttggaactgacagaaccg 
               
               
                   
               
               
                 caacgttgaaggagccactcagccgcgggtttctggagtttaatgagctaagcacatacgtcagaaaccattattgc 
               
               
                   
               
               
                 gcgttcaaaagtcgcctaaggtcactatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaa 
               
               
                   
               
               
                 ttcccctcggtatccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgca 
               
               
                   
               
               
                 tgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaa 
               
               
                   
               
               
                 cagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccga 
               
               
                   
               
               
                 cctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcg 
               
               
                   
               
               
                 cagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctg 
               
               
                   
               
               
                 tcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggc 
               
               
                   
               
               
                 tacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatc 
               
               
                   
               
               
                 aggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgac 
               
               
                   
               
               
                 ggcgatgatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggatt 
               
               
                   
               
               
                 catcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagc 
               
               
                   
               
               
                 ttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctat 
               
               
                   
               
               
                 cgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgacgcccaacctgccatca 
               
               
                   
               
               
                 cgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgat 
               
               
                   
               
               
                 cctccagcgcggggatctcatgctggagttcttcgcccacgggatctctgcggaacaggcggtcgaaggtgccgata 
               
               
                   
               
               
                 tcattacgacagcaacggccgacaagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatc 
               
               
                   
               
               
                 aacggcgtcggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcgtgga 
               
               
                   
               
               
                 gttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttg 
               
               
                   
               
               
                 ccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacg 
               
               
                   
               
               
                 ttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcg 
               
               
                   
               
               
                 cgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggc 
               
               
                   
               
               
                 tctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctctgaggg 
               
               
                   
               
               
                 aggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatga 
               
               
                   
               
               
                 ccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgct 
               
               
                   
               
               
                 gctatcgatggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaa 
               
               
                   
               
               
                 ttcccaaatggctcaagtcggtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctcc 
               
               
                   
               
               
                 ctcaatcggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccgattcat 
               
               
                   
               
               
                 taatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcac 
               
               
                   
               
               
                 tcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc 
               
               
                   
               
               
                 acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag cccacagatggttagagaggcttacgc   
               
               
                   
               
               
                 
                   agcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatg 
                 
               
               
                   
               
               
                 
                   cagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattcca 
                 
               
               
                   
               
               
                 
                   gtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccac 
                 
               
               
                   
               
               
                 
                   tgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttca 
                 
               
               
                   
               
               
                 
                   tacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactcc 
                 
               
               
                   
               
               
                 
                   aaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacct 
                 
               
               
                   
               
               
                 
                   cctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgcc 
                 
               
               
                   
               
               
                 
                   atcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacg 
                 
               
               
                   
               
               
                 
                   aggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgt 
                 
               
               
                   
               
               
                 
                   aagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaaca 
                 
               
               
                   
               
               
                   cgggg gactctaga GGATCC gtccggaattcccgggtcgacccacgcgtccgg gag attcagcgagataagcaattggattatctg 
               
               
                   
               
               
                 atgaaaggcttaaggcagcttggtccgcagttttcttccttagatgctaatcgaccttggctttgttactggattcttcattcaatagctttgc 
               
               
                   
               
               
                 ttggggagactgtggatgatgaattagaaagcaatgccattgacttccttggacgctgccagggctctgaaggtggatacggtggtggtcctggc 
               
               
                   
               
               
                 caacttccacatcttgcaactacttatgctgcagtgaatgcacttgttactttaggaggtgacaaagccctttcttcaattaatagagaaaaaat 
               
               
                   
               
               
                 gtcttgttttttaagacggatgaaggatacaagtggaggtttcaggatgcatgatatgggagaaatggatgttcgtgcatgctacactgcaattt 
               
               
                   
               
               
                 cggttgcaagcatcctaaatattatggatgatgaactcacccagggcctaggagattacatcttgagttgccaaacttatgaaggtggcattgga 
               
               
                   
               
               
                 ggggaacctggctccgaagctcacggtgggtatacctactgtggtttggctgctatgattttaatcaatgaggtcgaccgtttgaatttggattc 
               
               
                   
               
               
                 attaatgaattgggctgtacatcgacaaggagtagaaatgggatttcaaggtaggacgaacaaattggtcgatggttgctacacattttggca 
               
               
                   
               
               
                 ggcagccccttgtgttctactacaaagattatattcaaccaatgatcatgacgttcatggatcatcacatatatcagaagggacaaatgaagaa 
               
               
                   
               
               
                 catcatgctcatgatgaagatgaccttgaagacagtgatgatgatgatgattctgatgaggacaacgatgaagattcagtgaatggtcacaga 
               
               
                   
               
               
                 atccatcatacatccacctacattaacaggagaatgcaactggtttttgatagcctcggcttgcagagatatgtactcttgtgctctaagatcc 
               
               
                   
               
               
                 ctgacggtggattcagagacaagccgaggaaaccccgtgacttctaccacacatgttactgcctgagcggcttgtctgtggctcagcacgctt 
               
               
                   
               
               
                 ggttaaaagacgaggacactcctcctttgactcgcgacattatgggtggctactcgaatctccttgaacctgttcaacttcttcacaacattgtc 
               
               
                   
               
               
                 atggatcagtataatgaagctatcgagttcttctttaaagcagcatgaGGATCCctcgaatttccccgatcgttcaaacatttg 
               
               
                   
               
               
                 gcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgtta 
               
               
                   
               
               
                 agcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatac 
               
               
                   
               
               
                 atttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttact 
               
               
                   
               
               
                 agatcgggaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgcctt 
               
               
                   
               
               
                 gcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcag 
               
               
                   
               
               
                 cctgaatggcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctcta 
               
               
                   
               
               
                 aatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatgg 
               
               
                   
               
               
                 ttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggac 
               
               
                   
               
               
                 tcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcg 
               
               
                   
               
               
                 gaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccag 
               
               
                   
               
               
                 gcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccccagtacattaaaaacgtccgca 
               
               
                   
               
               
                 atgtgttattaagttgtctaagcgtcaattt gtttacaccacaatatatcctgcca   
               
               
                   
               
               
                 SEQ ID NO: 86 FORWARD Primer SacI site 5′ aaaCCCGGGatgccagtagtaacccgc 3′ 
               
               
                 SEQ ID NO: 171 REV Primer BamHI site 5′ aaaggatcctcatgctgctttaaagaagaactcgat 3′ 
               
               
                 SEQ ID NO: 172 Full length FT-B 
               
               
                 cccgggatgccagtagtaacccgcttgattcgtttgaagtgtgtagggctcagacttgaccggagtggactcaatcg 
               
               
                   
               
               
                 gcgaatctgtcacggaggacacggggaatcaacgcggcggagagtgatggaagagctttcaagcctaaccgtgagtc 
               
               
                   
               
               
                 agcgcgagcaatttctggtggagaacgatgtgttcgggatctataattacttcgacgccagcgacgtttctactcaa 
               
               
                   
               
               
                 aaatacatgatggagattcagcgagataagcaattggattatctgatgaaaggcttaaggcagcttggtccgcagtt 
               
               
                   
               
               
                 ttcttccttagatgctaatcgaccttggctttgttactggattcttcattcaatagctttgcttggggagactgtgg 
               
               
                   
               
               
                 atgatgaattagaaagcaatgccattgacttccttggacgctgccagggctctgaaggtggatacggtggtggtcct 
               
               
                   
               
               
                 ggccaacttccacatcttgcaactacttatgctgcagtgaatgcacttgttactttaggaggtgacaaagccctttc 
               
               
                   
               
               
                 ttcaattaatagagaaaaaatgtcttgttttttaagacggatgaaggatacaagtggaggtttcaggatgcatgata 
               
               
                   
               
               
                 tgggagaaatggatgttcgtgcatgctacactgcaatttcggttgcaagcatcctaaatattatggatgatgaactc 
               
               
                   
               
               
                 acccagggcctaggagattacatcttgagttgccaaacttatgaaggtggcattggaggggaacctggctccgaagc 
               
               
                   
               
               
                 tcacggtgggtatacctactgtggtttggctgctatgattttaatcaatgaggtcgaccgtttgaatttggattcat 
               
               
                   
               
               
                 taatgaattgggctgtacatcgacaaggagtagaaatgggatttcaaggtaggacgaacaaattggtcgatggttgc 
               
               
                   
               
               
                 tacacattttggcaggcagccccttgtgttctactacaaagattatattcaaccaatgatcatgacgttcatggatc 
               
               
                   
               
               
                 atcacatatatcagaagggacaaatgaagaacatcatgctcatgatgaagatgaccttgaagacagtgatgatgatg 
               
               
                   
               
               
                 atgattctgatgaggacaacgatgaagattcagtgaatggtcacagaatccatcatacatccacctacattaacagg 
               
               
                   
               
               
                 agaatgcaactggtttttgatagcctcggcttgcagagatatgtactcttgtgctctaagatccctgacggtggatt 
               
               
                   
               
               
                 cagagacaagccgaggaaaccccgtgacttctaccacacatgttactgcctgagcggcttgtctgtggctcagcacg 
               
               
                   
               
               
                 cttggttaaaagacgaggacactcctcctttgactcgcgacattatgggtggctactcgaatctccttgaacctgtt 
               
               
                   
               
               
                 caacttcttcacaacattgtcatggatcagtataatgaagctatcgagttcttctttaaagcagcatgaggatcc 
               
               
                   
               
               
                 SEQ ID NO: 177 Full Length FT-B amino acid sequence encoded by SEQ ID NO: 172 
               
               
                 MPVVTRLIRLKCVGLRLDRSGLNRRICHGGHGESTRRRVMEELSSLTVSQREQFLVENDVFGIYNYFDASDVSTQKY 
               
               
                   
               
               
                 MMEIQRDKQLDYLMKGLRQLGPQFSSLDANRPWLCYWILHSIALLGETVDDELESNAIDFLGRCQGSEGGYGGGPGQ 
               
               
                   
               
               
                 LPHLATTYAAVNALVTLGGDKALSSINREKMSCFLRRMKDTSGGFRMHDMGEMDVRACYTAISVASILNIMDDELTQ 
               
               
                   
               
               
                 GLGDYILSCQTYEGGIGGEPGSEAHGGYTYCGLAAMILINEVDRLNLDSLMNWAVHRQGVEMGFQGRTNKLVDGCYT 
               
               
                   
               
               
                 FWQAAPCVLLQRLYSTNDHDVHGSSHISEGTNEEHHAHDEDDLEDSDDDDDSDEDNDEDSVNGHRIHHTSTYINRRM 
               
               
                   
               
               
                 QLVFDSLGLQRYVLLCSKIPDGGFRDKPRKPRDFYHTCYCLSGLSVAQHAWLKDEDTPPLTRDIMGGYSNLLEPVQL 
               
               
                   
               
               
                 LHNIVMDQYNEAIEFFFKAA 
               
               
                   
               
               
                 SEQ ID NO: 173 pBI121-AtFTB (Full length vector Over-expression) 
               
               
                   gtttacccgccaatatatcctgtc aaacactgatagtttaaactgaaggcgggaaacgacaatctgatcatgagcgg 
               
               
                   
               
               
                 agaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttggaactgacagaaccg 
               
               
                   
               
               
                 caacgttgaaggagccactcagccgcgggtttctggagtttaatgagctaagcacatacgtcagaaaccattattgc 
               
               
                   
               
               
                 gcgttcaaaagtcgcctaaggtcactatcagctagcaaatatttcttgtcaaaaatgctccactgacgttccataaa 
               
               
                   
               
               
                 ttcccctcggtatccaattagagtctcatattcactctcaatccaaataatctgcaccggatctggatcgtttcgca 
               
               
                   
               
               
                 tgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaa 
               
               
                   
               
               
                 cagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccga 
               
               
                   
               
               
                 cctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcg 
               
               
                   
               
               
                 cagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctg 
               
               
                   
               
               
                 tcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggc 
               
               
                   
               
               
                 tacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatc 
               
               
                   
               
               
                 aggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgac 
               
               
                   
               
               
                 ggcgatgatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggatt 
               
               
                   
               
               
                 catcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagc 
               
               
                   
               
               
                 ttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctat 
               
               
                   
               
               
                 cgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgacgcccaacctgccatca 
               
               
                   
               
               
                 cgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgat 
               
               
                   
               
               
                 cctccagcgcggggatctcatgctggagttcttcgcccacgggatctctgcggaacaggcggtcgaaggtgccgata 
               
               
                   
               
               
                 tcattacgacagcaacggccgacaagcacaacgccacgatcctgagcgacaatatgatcgggcccggcgtccacatc 
               
               
                   
               
               
                 aacggcgtcggcggcgactgcccaggcaagaccgagatgcaccgcgatatcttgctgcgttcggatattttcgtgga 
               
               
                   
               
               
                 gttcccgccacagacccggatgatccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttg 
               
               
                   
               
               
                 ccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacg 
               
               
                   
               
               
                 ttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcg 
               
               
                   
               
               
                 cgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggcctcctgtcaatgctggcggcggc 
               
               
                   
               
               
                 tctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctctgaggg 
               
               
                   
               
               
                 aggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatga 
               
               
                   
               
               
                 ccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgct 
               
               
                   
               
               
                 gctatcgatggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaa 
               
               
                   
               
               
                 ttcccaaatggctcaagtcggtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctcc 
               
               
                   
               
               
                 ctcaatcggttgaatgtcgcccttttgtctttggcccaatacgcaaaccgcctctccccgcgcgttggccgattcat 
               
               
                   
               
               
                 taatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcac 
               
               
                   
               
               
                 tcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc 
               
               
                   
               
               
                 acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag cccacagatggttagagaggcttacgc   
               
               
                   
               
               
                 
                   agcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatg 
                 
               
               
                   
               
               
                 
                   cagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattcca 
                 
               
               
                   
               
               
                 
                   gtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccac 
                 
               
               
                   
               
               
                 
                   tgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttca 
                 
               
               
                   
               
               
                 
                   tacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactcc 
                 
               
               
                   
               
               
                 
                   aaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacct 
                 
               
               
                   
               
               
                 
                   cctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgcc 
                 
               
               
                   
               
               
                 
                   atcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacg 
                 
               
               
                   
               
               
                 
                   aggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgt 
                 
               
               
                   
               
               
                 
                   aagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaacacgg 
                 
               
               
                   
               
               
                   gg gactctagaggatcc CCCGGG atgccagtagtaacccgcttgattcgtttgaagtgtgtagggctca 
               
               
                   
               
               
                 gacttgaccggagtggactcaatcggcgaatctgtcacggaggacacggggaatcaacgcggcggagagtgatggaagagcttt 
               
               
                   
               
               
                 caagcctaaccgtgagtcagcgcgagcaatttctggtggagaacgatgtgttcgggatctataattacttcgacgccagcgacgttt 
               
               
                   
               
               
                 ctactcaaaaatacatgatggagattcagcgagataagcaattggattatctgatgaaaggcttaaggcagcttggtccgcagttttc 
               
               
                   
               
               
                 ttccttagatgctaatcgaccttggctttgttactggattcttcattcaatagctttgcttggggagactgtggatgatgaattagaaagcaa 
               
               
                   
               
               
                 tgccattgacttccttggacgctgccagggctctgaaggtggatacggtggtggtcctggccaacttccacatcttgcaactacttatgc 
               
               
                   
               
               
                 tgcagtgaatgcacttgttactttaggaggtgacaaagccctttcttcaattaatagagaaaaaatgtcttgttttttaagacggatgaagg 
               
               
                   
               
               
                 atacaagtggaggtttcaggatgcatgatatgggagaaatggatgttcgtgcatgctacactgcaatttcggttgcaagcatcctaaata 
               
               
                   
               
               
                 ttatggatgatgaactcacccagggcctaggagattacatcttgagttgccaaacttatgaaggtggcattggaggggaacctggctcc 
               
               
                   
               
               
                 gaggctcacggtgggtatacctactgtggtttggctgctatgattttaatcaatgaggtcgaccgtttgaatttggattcattaatgaattggg 
               
               
                   
               
               
                 ctgtacatcgacaaggagtagaaatgggatttcaaggtaggacgaacaaattggtcgatggttgctacacattttggcaggcagcccc 
               
               
                   
               
               
                 ttgtgttctactacaaagattatattcaaccaatgatcatgacgttcatggatcatcacatatatcagaagggacaaatgaagaacatcat 
               
               
                   
               
               
                 gctcatgatgaagatgaccttgaagacagtgatgatgatgatgattctgatgaggacaacgatgaagattcagtgaatggtcacagaa 
               
               
                   
               
               
                 tccatcatacatccacctacattaacaggagaatgcaactggtttttgatagcctcggcttgcagagatatgtactcttgtgctctaagat 
               
               
                   
               
               
                 ccctgacggtggattcagagacaagccgaggaaaccccgtgacttctaccacacatgttactgcctgagcggcttgtctgtggctca 
               
               
                   
               
               
                 gcacgcttggttaaaagacgaggacactcctcctttgactcgcgacattatgggtggctactcgaatctccttgaacctgttcaacttct 
               
               
                   
               
               
                 tcacaacattgtcatggatcagtataatgaagctatcgagttcttctttaaagcagcatgaGGATCCctcgaatttccccgatcgttc 
               
               
                   
               
               
                 aaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaa 
               
               
                   
               
               
                 taattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaat 
               
               
                   
               
               
                 atagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattcactggccgtcgttttacaacg 
               
               
                   
               
               
                 tcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaata 
               
               
                   
               
               
                 gcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgcccgctcctttcgctttcttccc 
               
               
                   
               
               
                 ttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtg 
               
               
                   
               
               
                 ctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtt 
               
               
                   
               
               
                 tttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctat 
               
               
                   
               
               
                 ctcgggctattcttttgatttataagggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctggg 
               
               
                   
               
               
                 gcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac 
               
               
                   
               
               
                 tggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaattt gtt   
               
               
                   
               
               
                 
                   tacaccacaatatatcctgcca 
                 
               
            
           
         
       
     
     Example 48 
     Cloning and Transformation of Isoprenylcysteine Carboxyl Methyltransferase 
     The  Arabidopsis  isoprenylcysteine carboxyl methyltransferase (ICMT) sequence was obtained by RT-PCR amplification using the protocol described above. The sequence was produced using the primer pair identified by SEQ ID NO:174 (5′-aaaggatccatgacagagatcttcagtgacacca-3′) and SEQ ID NO:175 (5′-aaagagctctcagttcacaaatggaacaccaga-3′). The sequence is identical to that reported by Accession number AB007648, GI: 10177821 (December 2000). 
     The isolated sequence was used to generate plant transformation vectors designed either to express the encoded protein or down-regulate expression. The vectors were used to transform  Arabidopsis  by the flower dipping method described elsewhere. Transformed plants were selected and propagated. Molecular and physiological analysis of the transgenic lines can be performed as detailed in other examples. Such analysis can include; molecular studies such as PCR, Southern, Northern and Western analysis; physiological analysis such as; growth studies, tolerance to environmental stress (drought, salt, heat, cold) tolerance to biotic stress, nutritional stress, as well as biochemical analysis. 
     
       
         
           
               
            
               
                 SEQ ID NO: 176 
               
            
           
           
               
            
               
                 atgacagagatcttcagtgacaccagcatcagacagttatctcaaatg 
               
               
                   
               
               
                 ctactatcactaatcttcttccacatatccgaatacattctagccatc 
               
               
                   
               
               
                 accattcacggagcatcaaacgtaactcttagttcgcttttaatcaccaa 
               
               
                   
               
               
                 gcattacgctttagcaatgcttctgtcgcttctcgaatacctaacggag 
               
               
                   
               
               
                 attatcctcttcccggggctgaaacaacactggtgggtcagcaacttt 
               
               
                   
               
               
                 ggactcataatgatcatcgttggggaaatcatcaggaaggcagcgata 
               
               
                   
               
               
                 ataacagcgggaagatcgttcactcacctcataaagatcaactacgaa 
               
               
                   
               
               
                 gagcatcacgggcttgtgactcatggtgtgtatagactaatgaggcat 
               
               
                   
               
               
                 ccaagttactgcggttttctcatctggtcggtcgggacacaagttatgct 
               
               
                   
               
               
                 ctgtaaccccgtttcagcagttgcgttcgcggttgtcgtgtggcggttt 
               
               
                   
               
               
                 tttgctcagagaataccgtacgaggagtattttctgaatcagttttttg 
               
               
                   
               
               
                 gggtacagtatctagagtatgcagagagtgttgcctctggtgttcc 
               
               
                   
               
               
                 atttgtgaactga 
               
            
           
         
       
     
     REFERENCES 
     
         
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     All citations in this application to materials and methods are hereby incorporated by reference. 
     EQUIVALENTS 
     Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described specifically herein. Such equivalents are intended to be encompassed in the scope of the following claims.