Patent Publication Number: US-2004052799-A1

Title: Nucleic acid and amino acid sequences relating to Helicobacter pylori for diagnostics and therapeutics

Description:
RELATED APPLICATIONS  
     [0001] The contents, including the Sequence Listings and Figures, of each of the following related applications listed by serial number and filing date is incorporated herein by reference.  
     [0002] This application is a continuation-in-part of U.S. Ser. No. 08/821,931, filed Mar. 21, 1997, which is a continuation of U.S. Ser. No. 08/761,184, filed Dec. 6, 1996 now abandoned, which is a continuation-in-part of U.S. Ser. No. 08/759,739, filed Dec. 6, 1996, which is a continuation-in-part of U.S. Ser. No. 08/736,791, filed Oct. 25, 1996, which is a continuation-in-part of U.S. Ser. No. 08/739,150, filed Oct. 28, 1996, which is a continuation-in-part of 08/660,742, filed Jun. 6, 1996, which is a continuation-in-part of U.S. Ser. No. 08/630,405, filed Apr. 1, 1996, which is a continuation-in-part of U.S. Ser. No. 08/561,469, filed Nov. 17, 1995, which is a continuation-in-part of U.S. Ser. No. 08/487,032, filed Jun. 7, 1995. This application also claims priority to PCT application PCT/US97/19575, filed Oct. 28, 1997, and PCT application PCT/US96/18542, filed Nov. 15, 1996. This application is also a continuation-in-part of U.S. Ser. No. 08/625,431 filed Mar. 26, 1996 which is a continuation-in-part of U.S. Ser. No. 08/621,425, filed Mar. 25, 1996, which is a continuation-in-part of U.S. Ser. No. 08/561,469, filed Nov. 17, 1995, which is a continuation-in-part of U.S. Ser. No. 08/487,032, filed Jun. 7, 1995. This application is also a continuation-in-part of U.S. Ser. No. 08/824,132, filed Mar. 27, 1997, which is a continuation-in-part of U.S. Ser. No. 08/761,318, filed Dec. 6, 1996, which is a continuation-in-part of U.S. Ser. No. 08/738,859, filed Oct. 28, 1996 now abandoned, which is a continuation-in-part of U.S. Ser. No. 08/625,811, filed Mar. 29, 1996. This application is also a continuation-in-part of U.S. Ser. No. 08/769,224, filed Dec. 6, 1996, which is a continuation-in-part of U.S. Ser. No. 08/736,905, filed Oct. 25, 1996 now abandoned, which is a continuation-in-part of U.S. Ser. No. 08/758,731, filed Apr. 2, 1996. This application is also a continuation-in-part of U.S. Ser. No. 08/823,745, filed Mar. 25, 1997, which is a continuation-in-part of U.S. Ser. No. 08/759,625, filed Dec. 5, 1996. This application also claims priority to a PCT application, number not yet available, filed Dec. 5, 1997, (Atty. Docket No. GTN-011CP2PC). This application is also a continuation-in-part of U.S. Ser. No. 08/761,066, filed Dec. 5, 1996. This application is also a continuation-in-part of U.S. Ser. No. 08/891,928, filed Jul. 14, 1997. This application is also a continuation-in-part of U.S. Ser. No. 08/892,020, filed Jul. 14, 1997.  
     [0003] It should be understood that all of the Sequence Listings and Figures of all of the aforementioned applications are considered to be part of the contents which are incorporated by reference herein. For example, FIG. 1 (pages 1-1199), which contains  H. pylori  genomic DNA, of U.S. Ser. No. 08/621,425, filed Mar. 25, 1996 is incorporated herein by reference. FIG. 1 (pages 1-1199), which contains  H. pylori  genomic DNA, and FIGS.  2 - 809  and FIG. 810 (pages 908-2700), which contain  H. pylori  amino acid sequences, of U.S. Ser. No. 08/625,431, filed Mar. 26, 1996 also are incorporated herein by reference. 
    
    
     
       BACKGROUND OF THE INVENTION  
       [0004] Helicobacter pylori  is a gram-negative, S-shaped, microaerophilic bacterium that was discovered and cultured from a human gastric biopsy specimen. (Warren, J. R. and B. Marshall, (1983)  Lancet  1: 1273-1275; and Marshall et al., (1984)  Microbios Lett.  25: 83-88).  H. pylori  has been strongly linked to chronic gastritis and duodenal ulcer disease. (Rathbone et. al., (1986)  Gut  27: 635-641). Moreover, evidence is accumulating for an etiologic role of  H. pylori  in nonulcer dyspepsia, gastric ulcer disease, and gastric adenocarcinoma. (Blaser M. J., (1993)  Trends Microbiol.  1: 255-260). Transmission of the bacteria occurs via the oral route, and the risk of infection increases with age. (Taylor, D. N. and M. J. Blaser, (1991)  Epidemiol. Rev  13: 42-50).  H. pylori  colonizes the human gastric mucosa, establishing an infection that usually persists for decades. Infection by  H. pylori  is prevalent worldwide. Developed countries have infection rates over 50% of the adult population, while developing countries have infection rates reaching 90% of the adults over the age of 20. (Hopkins R. J. and J. G. Morris (1994)  Am. J. Med.  97: 265-277).  
       [0005] The bacterial factors necessary for colonization of the gastric environment, and for virulence of this pathogen, are poorly understood. Examples of the putative virulence factors include the following: urease, an enzyme that may play a role in neutralizing gastric acid pH (Eaton et al., (1991)  Infect. Immunol.  59: 2470-2475; Ferrero, R. L. and A. Lee (1991)  Microb. Ecol. Hlth. Dis.  4: 121-134; Labigne et al., (1991)  J. Bacteriol.  173: 1920-1931); the bacterial flagellar proteins responsible for motility across the mucous layer. (Hazell et al., (1986)  J. Inf. Dis.  153: 658-663; Leying et al., (1992)  Mol. Microbiol.  6: 2863-2874; and Haas et al., (1993)  Mol. Microbiol.  8: 753-760); Vac A, a bacterial toxin that induces the formation of intracellular vacuoles in epithelial cells (Schmitt, W. and R. Haas, (1994)  Molecular Microbiol.  12(2): 307-319); and several gastric tissue-specific adhesins. (Boren et al., (1993)  Science  262: 1892-1895; Evans et al., (1993)  J. Bacteriol.  175: 674-683; and Falk et al., (1993)  Proc. Natl. Acad. Sci. USA  90: 2035-203).  
       [0006] Numerous therapeutic agents are currently available that eradicate  H. pylori  infections in vitro. (Huesca et. al., (1993)  Zbl. Bakt.  280: 244-252; Hopkins, R. J. and J. G. Morris, supra). However, many of these treatments are suboptimally effective in vivo because of bacterial resistance, altered drug distribution, patient non-compliance or poor drug availabilty. (Hopkins, R. J. and J. G. Morris, supra). Treatment with antibiotics combined with bismuth are part of the standard regime used to treat  H. pylori  infection. (Malfertheiner, P. and J. E. Dominguez-Munoz (1993)  Clinical Therapeutics  15 Supp. B: 37-48). Recently, combinations of a proton pump inhibitors and a single antibiotic have been shown to ameliorate duodenal ulcer disease. (Malfertheiner, P. and J. E. Dominguez-Munoz supra). However, methods employing antibiotic agents can have the problem of the emergence of bacterial strains which are resistant to these agents. (Hopkins, R. J. and J. G. Morris, supra). These limitations demonstrate that new more effective methods are needed to combat  H. pylori  infections in vivo. In particular, the design of new vaccines that may prevent infection by this bacterium is highly desirable.  
       SUMMARY OF THE INVENTION  
       [0007] This invention relates to novel genes, e.g., genes encoding polypeptides such as bacterial surface proteins, from the organism  Helicobacter pylori  ( H. pylori ), and other related genes, their products, and uses thereof. The nucleic acids and peptides of the present invention have utility for diagnostic and therapeutics for  H. pylori  and other Helicobacter species. They can also be used to detect the presence of  H. pylori  and other Helicobacter species in a sample; and for use in screening compounds for the ability to interfere with the  H. pylori  life cycle or to inhibit  H. pylori  infection. More specifically, this invention features compositions of nucleic acids corresponding to entire coding sequences of  H. pylori  proteins, including surface or secreted proteins or parts thereof, nucleic acids capable of binding mRNA from  H. pylori  proteins to block protein translation, and methods for producing  H. pylori  proteins or parts thereof using peptide synthesis and recombinant DNA techniques. This invention also features antibodies and nucleic acids useful as probes to detect  H. pylori  infection. In addition, compositions, including vaccine compositions, and methods for the protection or treatment of infection by  H. pylori  are within the scope of this invention. 
     
    
    
     DETAILED DESCRIPTION OF THE DRAWINGS  
     [0008]FIG. 1 is a bar graph that depicts the antibody titer in serum of mice following immunization with specific  H. pylori  antigens.  
     [0009]FIG. 2 is a bar graph that depicts the antibody titer in mucous of mice following immunization with specific  H. pylori  antigens.  
     [0010]FIG. 3 is a bar graph that depicts therapeutic immunization of  H. pylori  infected mice with specific antigens dissolved in HEPES buffer.  
     [0011]FIG. 4 is a bar graph that depicts therapeutic immunization of  H. pylori  infected mice with specific antigens dissolved in buffer containing DOC.  
     [0012]FIG. 5 is a graph depicting the activity of recombinant PPIase.  
     [0013]FIG. 6 is a graph depicting PPIase activity in an  H. pylori  extract.  
     [0014]FIG. 7 is a graph depicting inhibition of glutamate racemase activity by L-Serine-O-Sulfate.  
     [0015]FIG. 8 is a graph depicting the results of an assay of the 3-deoxy-D-manno-2-octulosonic-8-phosphate (KDO-8-P) catalyzed reaction.  
     [0016]FIG. 9 is a graph depicting the stability of 3-deoxy-D-manno-2-octulosonic-8-phosphate (KDO-8-P) after purification.  
     [0017]FIG. 10 depicts predicted amphipathic beta-sheet regions at the C-terminus as well as additional amino acid sequence motifs of ten  H. pylori  outer membrane proteins.  
     [0018]FIG. 11 depicts predicted amphipathic beta-sheet regions at the C-terminus as well as additional amino acid sequence motifs of five  H. pylori  outer membrane proteins.  
     [0019]FIG. 12 depicts predicted amphipathic beta-sheet regions at the C-terminus as well as additional amino acid sequence motifs of five  H. pylori  outer membrane proteins.  
     [0020]FIG. 13 depicts amino acid sequence motifs of twelve  H. pylori  outer membranes.  
     [0021]FIG. 14 depicts sequence similarities in the N-terminal portion of six  H. pylori  proteins.  
     [0022]FIG. 15 depicts two members of a family of  H. pylori  outer membrane proteins which share significant homology across most of their sequences.  
     [0023]FIG. 16 depicts two members of a family of  H. pylori  outer membrane proteins which share significant homology across most of their sequences.  
     [0024]FIG. 17 depicts five members of a family of  H. pylori  outer membrane proteins which share significant homology across most of their sequences.  
    
    
     DETAILED DESCRIPTION OF THE INVENTION  
     [0025] In one aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide of SEQ ID NO: 4763. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide of SEQ ID NO: 4763, such nucleic acid is contained in SEQ ID NO: 1. The  H. pylori  polypeptide sequences of the invention described herein are contained in the Sequence Listing, and the nucleic acids encoding  H. pylori  polypeptides of the invention are contained in the Sequence Listing.  
     [0026] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 4763 through SEQ ID NO: 5012. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 4763 through SEQ ID NO: 5012, such nucleic acids are contained in SEQ ID NO: 1 through SEQ ID NO:250.  
     [0027] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 5013 through SEQ ID NO: 5262. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 5013 through SEQ ID NO: 5262, such nucleic acids are contained in SEQ ID NO: 251 through SEQ ID NO:500.  
     [0028] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 5263 through SEQ ID NO: 5512. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 5263 through SEQ ID NO: 5512, such nucleic acids are contained in SEQ ID NO: 501 through SEQ ID NO:750.  
     [0029] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 5513 through SEQ ID NO: 5762. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 5513 through SEQ ID NO: 5762, such nucleic acids are contained in SEQ ID NO: 751 through SEQ ID NO:1000.  
     [0030] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 5763 through SEQ ID NO: 6012. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 5763 through SEQ ID NO: 6012, such nucleic acids are contained in SEQ ID NO: 1001 through SEQ ID NO:1250.  
     [0031] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 6013 through SEQ ID NO: 6262 The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 6013 through SEQ ID NO: 6262, such nucleic acids are contained in SEQ ID NO: 1125 through SEQ ID NO:1500.  
     [0032] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 6263 through SEQ ID NO: 6512. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 6263 through SEQ ID NO: 6512, such nucleic acids are contained in SEQ ID NO: 1501 through SEQ ID NO:1750.  
     [0033] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 6513 through SEQ ID NO: 6762. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 6513 through SEQ ID NO: 6762, such nucleic acids are contained in SEQ ID NO: 1751 through SEQ ID NO:2000.  
     [0034] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 6763 through SEQ ID NO: 7012. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 6763 through SEQ ID NO: 7012, such nucleic acids are contained in SEQ ID NO: 2001 through SEQ ID NO:2250.  
     [0035] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 7013 through SEQ ID NO: 7262. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 7013 through SEQ ID NO: 7262, such nucleic acids are contained in SEQ ID NO: 2251 through SEQ ID NO:2500.  
     [0036] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO: 7263 through SEQ ID NO:7512. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 7263 through SEQ ID NO: 7512, such nucleic acids are contained in SEQ ID NO: 2501 through SEQ ID NO:2750.  
     [0037] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO:7513 through SEQ ID NO:7762. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 7513 through SEQ ID NO: 7762, such nucleic acids are contained in SEQ ID NO: 2751 through SEQ ID NO:3000.  
     [0038] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO:7763 through SEQ ID NO:8012. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 7763 through SEQ ID NO: 8012, such nucleic acids are contained in SEQ ID NO: 3001 through SEQ ID NO:3250.  
     [0039] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO:8013 through SEQ ID NO:8262. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO:8013 through SEQ ID NO: 8262, such nucleic acids are contained in SEQ ID NO: 3251 through SEQ ID NO:3500.  
     [0040] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO:8263 through SEQ ID NO:8512. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO:8263 through SEQ ID NO: 8512, such nucleic acids are contained in SEQ ID NO: 3501 through SEQ ID NO:3750.  
     [0041] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO:8513 through SEQ ID NO:8762 The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO:8513 through SEQ ID NO: 8762, such nucleic acids are contained in SEQ ID NO: 3751 through SEQ ID NO:4000.  
     [0042] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO:8763 through SEQ ID NO:9012. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO:8763 through SEQ ID NO: 9012, such nucleic acids are contained in SEQ ID NO: 4001 through SEQ ID NO:4250.  
     [0043] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO:9013 through SEQ ID NO:9262 The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO:9013 through SEQ ID NO: 9262, such nucleic acids are contained in SEQ ID NO: 4251 through SEQ ID NO:4500.  
     [0044] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO:9263 through SEQ ID NO:9512. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO:9263 through SEQ ID NO: 9512, such nucleic acids are contained in SEQ ID NO: 4501 through SEQ ID NO:4750.  
     [0045] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO:9513 through SEQ ID NO:9524. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO:9513 through SEQ ID NO: 9524, such nucleic acids are contained in SEQ ID NO: 4751 through SEQ ID NO:4762.  
     [0046] In another aspect, the invention features a recombinant or substantially pure preparation of  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides of SEQ ID NO:9637 through SEQ ID NO: 9798. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 9637 through SEQ ID NO: 9798, such nucleic acids are contained in SEQ ID NO: 9525 through SEQ ID 9636.  
     [0047] In another aspect, the invention features a recombinant or substantially pure preparation of an  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides as set forth in the Sequence Listing. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide selected from the group consisting of  H. pylori  polypeptides as set forth in the Sequence Listing. It should be understood that this invention encompasses each of the  H. pylori  polypeptides and nucleic acids encoding such polypeptides as identified in the Sequence Listing by a given sequence identification number. For example, a representative  H. pylori  polypeptide is contained in SEQ ID NO: 4763. Therefore, this invention encompasses a recombinant or substantially pure preparation of an  H. pylori  polypeptide of SEQ ID NO: 4763. The invention also includes substantially pure nucleic acid encoding an  H. pylori  polypeptide of SEQ ID NO: 4763.  
     [0048] In another aspect, the invention pertains to any individual  H. pylori  polypeptide member or nucleic acid encoding such member from the above-identified groups of  H. pylori  polypeptides (e.g., SEQ ID NO: 4763-SEQ ID NO: 9524 and SEQ ID NO: 9637-SEQ ID NO: 9748) or nucleic acids (e.g., SEQ ID NO: 1-SEQ ID NO: 4762 and SEQ ID NO: 9525-SEQ ID NO: 9636), as well as any subgroups from within the above-identified groups. Furthermore, the subgroups can preferably consist of 1, 3, 5, 10, 15, 20, 30, 40, 50, 75, 100, 125, 150, 175, 200 or 225 members of any of the groups identified above, as well as, any combinations thereof. For example, the group consisting of  H. pylori  polypeptides SEQ ID NO: 4763 through SEQ ID NO: 5012 can be divided into one or more subgroups as follows: SEQ ID NO: 4763-SEQ ID NO: 4800; SEQ ID NO: 4801-SEQ ID NO: 4860; SEQ ID NO: 4861-SEQ ID NO: 4950; SEQ ID NO: 4951-SEQ ID NO: 5012; or any combinations thereof.  
     [0049] Particularly preferred  H. pylori  polypeptide or fragments thereof comprises a purified  H. pylori murI  polypeptide or a fragment thereof, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 7635. The invention also includes an isolated nucleic acid encoding a  H. pylori murI  polypeptide, which nucleic acid comprises the nucleotide sequence of SEQ ID NO: 2873.  
     [0050] Particularly preferred  H. pylori  polypeptide or fragments thereof comprises a purified  H. pylori murC  polypeptide or a fragment thereof, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 7607. The invention also includes an isolated nucleic acid encoding a  H. pylori murC  polypeptide, which nucleic acid comprises the nucleotide sequence of SEQ ID NO: 2845.  
     [0051] In another aspect, the invention features an isolated nucleic acid having a nucleotide sequence encoding an  H. pylori  polypeptide at least about 60% homologous to an amino acid sequence selected from the group consisting of SEQ ID NO: 4763-SEQ ID NO: 9524 and SEQ ID NO: 9637-SEQ ID NO: 9748. In a preferred embodiment, the isolated nucleic acid includes a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-SEQ ID NO: 4762 and SEQ ID NO: 9525-SEQ ID NO: 9636, or a complement thereof.  
     [0052] In another aspect, the invention features an isolated nucleic acid having a nucleotide sequence encoding an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 4763-SEQ ID NO: 9524 and SEQ ID NO: 9637-SEQ ID NO: 9748.  
     [0053] In another aspect, the invention features an isolated nucleic acid which encodes an  H. pylori  polypeptide, having a nucleotide sequence at least about 60% homologous to a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-SEQ ID NO: 4762 and SEQ ID NO: 9525-SEQ ID NO: 9636, or a complement thereof.  
     [0054] In another aspect, the invention features an isolated nucleic acid molecule encoding an  H. pylori  polypeptide, having a nucleotide sequence which hybridizes under stringent hybridization conditions to a nucleic acid molecule having the nucleotide sequence selected from the group consisting of SEQ ID NO: 1-SEQ ID NO: 4762 and SEQ ID NO: 9525-SEQ ID NO: 9636, or a complement thereof.  
     [0055] In another aspect, the invention features an isolated nucleic acid having a nucleotide sequence of at least 8 nucleotides in length, wherein the sequence hybridizes under stringent hybridization conditions to a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-SEQ ID NO: 4762 and SEQ ID NO: 9525-SEQ ID NO: 9636, or a complement thereof.  
     [0056] Particularly preferred is an isolated nucleic acid having a nucleotide sequence encoding an  H. pylori  cell envelope polypeptide or a fragment thereof, the nucleic acid selected from the group consisting of SEQ ID NO: 1; SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 5; SEQ ID NO: 6; SEQ ID NO: 7; SEQ ID NO: 8; SEQ ID NO: 9; SEQ ID NO: 10; SEQ ID NO: 11; SEQ ID NO: 12; SEQ ID NO: 13; SEQ ID NO: 14; SEQ ID NO: 15; SEQ ID NO: 16; SEQ ID NO: 17; SEQ ID NO: 18; SEQ ID NO: 19; SEQ ID NO: 20; SEQ ID NO: 21; SEQ ID NO: 22; SEQ ID NO: 23; SEQ ID NO: 24; SEQ ID NO: 25; SEQ ID NO: 26; SEQ ID NO: 27; SEQ ID NO: 28; SEQ ID NO: 29; SEQ ID NO: 30; SEQ ID NO: 31; SEQ ID NO: 32; SEQ ID NO: 33; SEQ ID NO: 34; SEQ ID NO: 35; SEQ ID NO: 36; SEQ ID NO: 37; SEQ ID NO: 38; SEQ ID NO: 39; SEQ ID NO: 40; SEQ ID NO: 41; SEQ ID NO: 42; SEQ ID NO: 43; SEQ ID NO: 44; SEQ ID NO: 45; SEQ ID NO: 46; SEQ ID NO: 47; SEQ ID NO: 48; SEQ ID NO: 49; SEQ ID NO: 50; SEQ ID NO: 51; SEQ ID NO: 52; SEQ ID NO: 53; SEQ ID NO: 54; SEQ ID NO: 55; SEQ ID NO: 56; SEQ ID NO: 57; SEQ ID NO: 58; SEQ ID NO: 59; SEQ ID NO: 60; SEQ ID NO: 61; SEQ ID NO: 62; SEQ ID NO: 63; SEQ ID NO: 64; SEQ ID NO: 65; SEQ ID NO: 66; SEQ ID NO: 67; SEQ ID NO: 68; SEQ ID NO: 69; SEQ ID NO: 70; SEQ ID NO: 71; SEQ ID NO: 72; SEQ ID NO: 73; SEQ ID NO: 74; SEQ ID NO: 75; SEQ ID NO: 76; SEQ ID NO: 77; SEQ ID NO: 78; SEQ ID NO: 79; SEQ ID NO: 80; SEQ ID NO: 81; SEQ ID NO: 82; SEQ ID NO: 83; SEQ ID NO: 84; SEQ ID NO: 85; SEQ ID NO: 86; SEQ ID NO: 87; SEQ ID NO: 88; SEQ ID NO: 89; SEQ ID NO: 90; SEQ ID NO: 91; SEQ ID NO: 92; SEQ ID NO: 93; SEQ ID NO: 94; SEQ ID NO: 95; SEQ ID NO: 96; SEQ ID NO: 97; SEQ ID NO: 98; SEQ ID NO: 99; SEQ ID NO: 100; SEQ ID NO: 101; SEQ ID NO: 102; SEQ ID NO: 103; SEQ ID NO: 104; SEQ ID NO: 105; SEQ ID NO: 106; SEQ ID NO: 107; SEQ ID NO: 108; SEQ ID NO: 109; SEQ ID NO: 110; SEQ ID NO: 11; SEQ ID NO: 112; SEQ ID NO: 113; SEQ ID NO: 114; SEQ ID NO: 115; SEQ ID NO: 116; SEQ ID NO: 117; SEQ ID NO: 118; SEQ ID NO: 119; SEQ ID NO: 120; SEQ ID NO: 121; SEQ ID NO: 122; SEQ ID NO: 123; SEQ ID NO: 124; SEQ ID NO: 125; SEQ ID NO: 126; SEQ ID NO: 127; SEQ ID NO: 128; SEQ ID NO: 129; SEQ ID NO: 130; SEQ ID NO: 131; SEQ ID NO: 132; SEQ ID NO: 133; SEQ ID NO: 134; SEQ ID NO: 135; SEQ ID NO: 136; SEQ ID NO: 137; SEQ ID NO: 138; SEQ ID NO: 139; SEQ ID NO: 140; SEQ ID NO: 141; SEQ ID NO: 142; SEQ ID NO: 143; SEQ ID NO: 144; SEQ ID NO: 145; SEQ ID NO: 146; SEQ ID NO: 147; SEQ ID NO: 148; SEQ ID NO: 149; SEQ ID NO: 150; SEQ ID NO: 151; SEQ ID NO: 152; SEQ ID NO: 153; SEQ ID NO: 154; SEQ ID NO: 155; SEQ ID NO: 156; SEQ ID NO: 157; SEQ ID NO: 158; SEQ ID NO: 159; SEQ ID NO: 160; SEQ ID NO: 161; SEQ ID NO: 162; SEQ ID NO: 163; SEQ ID NO: 164; SEQ ID NO: 165; SEQ ID NO: 166; SEQ ID NO: 167; SEQ ID NO: 168; SEQ ID NO: 169; SEQ ID NO: 170; SEQ ID NO: 171; SEQ ID NO: 172; SEQ ID NO: 173; SEQ ID NO: 174; SEQ ID NO: 175; SEQ ID NO: 176; SEQ ID NO: 177; SEQ ID NO: 178; SEQ ID NO: 179; SEQ ID NO: 180; SEQ ID NO: 181; SEQ ID NO: 182; SEQ ID NO: 183; SEQ ID NO: 184; SEQ ID NO: 185; SEQ ID NO: 186; SEQ ID NO: 187; SEQ ID NO: 188; SEQ ID NO: 189; SEQ ID NO: 190; SEQ ID NO: 191; SEQ ID NO: 192; SEQ ID NO: 193; SEQ ID NO: 194; SEQ ID NO: 195; SEQ ID NO: 196; SEQ ID NO: 197; SEQ ID NO: 198; SEQ ID NO: 199; SEQ ID NO: 200; SEQ ID NO: 201; SEQ ID NO: 202; SEQ ID NO: 203; SEQ ID NO: 204; SEQ ID NO: 205; SEQ ID NO: 206; SEQ ID NO: 207; SEQ ID NO: 208; SEQ ID NO: 209; SEQ ID NO: 210; SEQ ID NO: 211; SEQ ID NO: 212; SEQ ID NO: 213; SEQ ID NO: 214; SEQ ID NO: 215; SEQ ID NO: 216; SEQ ID NO: 217; SEQ ID NO: 218; SEQ ID NO: 219; SEQ ID NO: 220; SEQ ID NO: 221; SEQ ID NO: 222; SEQ ID NO: 223; SEQ ID NO: 224; SEQ ID NO: 225; SEQ ID NO: 226; SEQ ID NO: 227; SEQ ID NO: 228; SEQ ID NO: 229; SEQ ID NO: 230; SEQ ID NO: 231; SEQ ID NO: 232; SEQ ID NO: 233; SEQ ID NO: 234; SEQ ID NO: 235; SEQ ID NO: 236; SEQ ID NO: 237; SEQ ID NO: 238; SEQ ID NO: 239; SEQ ID NO: 240; SEQ ID NO: 241; SEQ ID NO: 242; SEQ ID NO: 243; SEQ ID NO: 244; SEQ ID NO: 245; SEQ ID NO: 246; SEQ ID NO: 247; SEQ ID NO: 248; SEQ ID NO: 249; SEQ ID NO: 250; SEQ ID NO: 251; SEQ ID NO: 252; SEQ ID NO: 253; SEQ ID NO: 254; SEQ ID NO: 255; SEQ ID NO: 256; SEQ ID NO: 257; SEQ ID NO: 258; SEQ ID NO: 259; SEQ ID NO: 260; SEQ ID NO: 261; SEQ ID NO: 262; SEQ ID NO: 263; SEQ ID NO: 264; SEQ ID NO: 265; SEQ ID NO: 266; SEQ ID NO: 267; SEQ ID NO: 268; SEQ ID NO: 269; SEQ ID NO: 270; SEQ ID NO: 271; SEQ ID NO: 272; SEQ ID NO: 273; SEQ ID NO: 274; SEQ ID NO: 275; SEQ ID NO: 276; SEQ ID NO: 277; SEQ ID NO: 278; SEQ ID NO: 279; SEQ ID NO: 280; SEQ ID NO: 281; SEQ ID NO: 282; SEQ ID NO: 283; SEQ ID NO: 284; SEQ ID NO: 285; SEQ ID NO: 286; SEQ ID NO: 287; SEQ ID NO: 288; SEQ ID NO: 289; SEQ ID NO: 290; SEQ ID NO: 291; SEQ ID NO: 292; SEQ ID NO: 293; SEQ ID NO: 294; SEQ ID NO: 295; SEQ ID NO: 296; SEQ ID NO: 297; SEQ ID NO: 298; SEQ ID NO: 299; SEQ ID NO: 300; SEQ ID NO: 301; SEQ ID NO: 302; SEQ ID NO: 303; SEQ ID NO: 304; SEQ ID NO: 305; SEQ ID NO: 306; SEQ ID NO: 307; SEQ ID NO: 308; SEQ ID NO: 309; SEQ ID NO: 310; SEQ ID NO: 311; SEQ ID NO: 312; SEQ ID NO: 313; SEQ ID NO: 314; SEQ ID NO: 315; SEQ ID NO: 316; SEQ ID NO: 317; SEQ ID NO: 318; SEQ ID NO: 319; SEQ ID NO: 320; SEQ ID NO: 321; SEQ ID NO: 322; SEQ ID NO: 323; SEQ ID NO: 324; SEQ ID NO: 325; SEQ ID NO: 326; SEQ ID NO: 327; SEQ ID NO: 328; SEQ ID NO: 329; SEQ ID NO: 330; SEQ ID NO: 331; SEQ ID NO: 332; SEQ ID NO: 333; SEQ ID NO: 334; SEQ ID NO: 335; SEQ ID NO: 336; SEQ ID NO: 337; SEQ ID NO: 338; SEQ ID NO: 339; SEQ ID NO: 340; SEQ ID NO: 341; SEQ ID NO: 342; SEQ ID NO: 343; SEQ ID NO: 344; SEQ ID NO: 345; SEQ ID NO: 346; SEQ ID NO: 347; SEQ ID NO: 348; SEQ ID NO: 349; SEQ ID NO: 350; SEQ ID NO: 351; SEQ ID NO: 352; SEQ ID NO: 353; SEQ ID NO: 354; SEQ ID NO: 355; SEQ ID NO: 356; SEQ ID NO: 357; SEQ ID NO: 358; SEQ ID NO: 359; SEQ ID NO: 360; SEQ ID NO: 361; SEQ ID NO: 362; SEQ ID NO: 363; SEQ ID NO: 364; SEQ ID NO: 365; SEQ ID NO: 366; SEQ ID NO: 367; SEQ ID NO: 368; SEQ ID NO: 369; SEQ ID NO: 370; SEQ ID NO: 371; SEQ ID NO: 372; SEQ ID NO: 373; SEQ ID NO: 374; SEQ ID NO: 375; SEQ ID NO: 376; SEQ ID NO: 377; SEQ ID NO: 378; SEQ ID NO: 379; SEQ ID NO: 380; SEQ ID NO: 381; SEQ ID NO: 382; SEQ ID NO: 383; SEQ ID NO: 384; SEQ ID NO: 385; SEQ ID NO: 386; SEQ ID NO: 387; SEQ ID NO: 388; SEQ ID NO: 389; SEQ ID NO: 390; SEQ ID NO: 391; SEQ ID NO: 392; SEQ ID NO: 393; SEQ ID NO: 394; SEQ ID NO: 395; SEQ ID NO: 396; SEQ ID NO: 397; SEQ ID NO: 398; SEQ ID NO: 399; SEQ ID NO: 400; SEQ ID NO: 401; SEQ ID NO: 402; SEQ ID NO: 403; SEQ ID NO: 404; SEQ ID NO: 405; SEQ ID NO: 406; SEQ ID NO: 407; SEQ ID NO: 408; SEQ ID NO: 409; SEQ ID NO: 410; SEQ ID NO: 411; SEQ ID NO: 412; SEQ ID NO: 413; SEQ ID NO: 414; SEQ ID NO: 415; SEQ ID NO: 416; SEQ ID NO: 417; SEQ ID NO: 418; SEQ ID NO: 419; SEQ ID NO: 420; SEQ ID NO: 421; SEQ ID NO: 422; SEQ ID NO: 423; SEQ ID NO: 424; SEQ ID NO: 425; SEQ ID NO: 426; SEQ ID NO: 427; SEQ ID NO: 428; SEQ ID NO: 429; SEQ ID NO: 430; SEQ ID NO: 431; SEQ ID NO: 432; SEQ ID NO: 433; SEQ ID NO: 434; SEQ ID NO: 435; SEQ ID NO: 436; SEQ ID NO: 437; SEQ ID NO: 438; SEQ ID NO: 439; SEQ ID NO: 440; SEQ ID NO: 441; SEQ ID NO: 442; SEQ ID NO: 443; SEQ ID NO: 444; SEQ ID NO: 445; SEQ ID NO: 446; SEQ ID NO: 447; SEQ ID NO: 448; SEQ ID NO: 449; SEQ ID NO: 450; SEQ ID NO: 451; SEQ ID NO: 452; SEQ ID NO: 453; SEQ ID NO: 454; SEQ ID NO: 455; SEQ ID NO: 456; SEQ ID NO: 457; SEQ ID NO: 458; SEQ ID NO: 459; SEQ ID NO: 460; SEQ ID NO: 461; SEQ ID NO: 462; SEQ ID NO: 463; SEQ ID NO: 464; SEQ ID NO: 465; SEQ ID NO: 466; SEQ ID NO: 467; SEQ ID NO: 468; SEQ ID NO: 469; SEQ ID NO: 470; SEQ ID NO: 471; SEQ ID NO: 472; SEQ ID NO: 473; SEQ ID NO: 474; SEQ ID NO: 475; SEQ ID NO: 476; SEQ ID NO: 477; SEQ ID NO: 478; SEQ ID NO: 479; SEQ ID NO: 480; SEQ ID NO: 481; SEQ ID NO: 482; SEQ ID NO: 483; SEQ ID NO: 484; SEQ ID NO: 485; SEQ ID NO: 486; SEQ ID NO: 487; SEQ ID NO: 488; SEQ ID NO: 489; SEQ ID NO: 490; SEQ ID NO: 491; SEQ ID NO: 492; SEQ ID NO: 493; SEQ ID NO: 494; SEQ ID NO: 495; SEQ ID NO: 496; SEQ ID NO: 497; SEQ ID NO: 498; SEQ ID NO: 499; SEQ ID NO: 500; SEQ ID NO: 501; SEQ ID NO: 502; SEQ ID NO: 503; SEQ ID NO: 504; SEQ ID NO: 505; SEQ ID NO: 506; SEQ ID NO: 507; SEQ ID NO: 508; SEQ ID NO: 509; SEQ ID NO: 510; SEQ ID NO: 511; SEQ ID NO: 512; SEQ ID NO: 513; SEQ ID NO: 514; SEQ ID NO: 515; SEQ ID NO: 516; SEQ ID NO: 517; SEQ ID NO: 518; SEQ ID NO: 519; SEQ ID NO: 520; SEQ ID NO: 521; SEQ ID NO: 522; SEQ ID NO: 523; SEQ ID NO: 524; SEQ ID NO: 525; SEQ ID NO: 526; SEQ ID NO: 527; SEQ ID NO: 528; SEQ ID NO: 529; SEQ ID NO: 530; SEQ ID NO: 531; SEQ ID NO: 532; SEQ ID NO: 533; SEQ ID NO: 534; SEQ ID NO: 535; SEQ ID NO: 536; SEQ ID NO: 537; SEQ ID NO: 538; SEQ ID NO: 539; SEQ ID NO: 540; SEQ ID NO: 541; SEQ ID NO: 542; SEQ ID NO: 543; SEQ ID NO: 544; SEQ ID NO: 545; SEQ ID NO: 546; SEQ ID NO: 547; SEQ ID NO: 548; SEQ ID NO: 549; SEQ ID NO: 550; SEQ ID NO: 551; SEQ ID NO: 552; SEQ ID NO: 553; SEQ ID NO: 554; SEQ ID NO: 555; SEQ ID NO: 556; SEQ ID NO: 557; SEQ ID NO: 558; SEQ ID NO: 559; SEQ ID NO: 560; SEQ ID NO: 561; SEQ ID NO: 562; SEQ ID NO: 563; SEQ ID NO: 564; SEQ ID NO: 565; SEQ ID NO: 566; SEQ ID NO: 567; SEQ ID NO: 568; SEQ ID NO: 569; SEQ ID NO: 570; SEQ ID NO: 571; SEQ ID NO: 572; SEQ ID NO: 573; SEQ ID NO: 574; SEQ ID NO: 575; SEQ ID NO: 576; SEQ ID NO: 577; SEQ ID NO: 578; SEQ ID NO: 579; SEQ ID NO: 580; SEQ ID NO: 581; SEQ ID NO: 582; SEQ ID NO: 583; SEQ ID NO: 584; SEQ ID NO: 585; SEQ ID NO: 586; SEQ ID NO: 587; SEQ ID NO: 588; SEQ ID NO: 589; SEQ ID NO: 590; SEQ ID NO: 591; SEQ ID NO: 592; SEQ ID NO: 593; SEQ ID NO: 594; SEQ ID NO: 595; SEQ ID NO: 596; SEQ ID NO: 597; SEQ ID NO: 598; SEQ ID NO: 599; SEQ ID NO: 600; SEQ ID NO: 601; SEQ ID NO: 602; SEQ ID NO: 603; SEQ ID NO: 604; SEQ ID NO: 605; SEQ ID NO: 606; SEQ ID NO: 607; SEQ ID NO: 608; SEQ ID NO: 609; SEQ ID NO: 610; SEQ ID NO: 611; SEQ ID NO: 612; SEQ ID NO: 613; SEQ ID NO: 614; SEQ ID NO: 615; SEQ ID NO: 616; SEQ ID NO: 617; SEQ ID NO: 618; SEQ ID NO: 619; SEQ ID NO: 620; SEQ ID NO: 621; SEQ ID NO: 622; SEQ ID NO: 623; SEQ ID NO: 624; SEQ ID NO: 625; SEQ ID NO: 626; SEQ ID NO: 627; SEQ ID NO: 628; SEQ ID NO: 629; SEQ ID NO: 630; SEQ ID NO: 631; SEQ ID NO: 632; SEQ ID NO: 633; SEQ ID NO: 634; SEQ ID NO: 635; SEQ ID NO: 636; SEQ ID NO: 637; SEQ ID NO: 638; SEQ ID NO: 639; SEQ ID NO: 640; SEQ ID NO: 641; SEQ ID NO: 642; SEQ ID NO: 643; SEQ ID NO: 644; SEQ ID NO: 645; SEQ ID NO: 646; SEQ ID NO: 647; SEQ ID NO: 648; SEQ ID NO: 649; SEQ ID NO: 650; SEQ ID NO: 651; SEQ ID NO: 652; SEQ ID NO: 653; SEQ ID NO: 654; SEQ ID NO: 655; SEQ ID NO: 656; SEQ ID NO: 657; SEQ ID NO: 658; SEQ ID NO: 659; SEQ ID NO: 660; SEQ ID NO: 661; SEQ ID NO: 662; SEQ ID NO: 663; SEQ ID NO: 664; SEQ ID NO: 665; SEQ ID NO: 666; SEQ ID NO: 667; SEQ ID NO: 668; SEQ ID NO: 669; SEQ ID NO: 670; SEQ ID NO: 671; SEQ ID NO: 672; SEQ ID NO: 673; SEQ ID NO: 674; SEQ ID NO: 675; SEQ ID NO: 676; SEQ ID NO: 677; SEQ ID NO: 678; SEQ ID NO: 679; SEQ ID NO: 680; SEQ ID NO: 681; SEQ ID NO: 682; SEQ ID NO: 683; SEQ ID NO: 684; SEQ ID NO: 685; SEQ ID NO: 686; SEQ ID NO: 687; SEQ ID NO: 688; SEQ ID NO: 689; SEQ ID NO: 690; SEQ ID NO: 691; SEQ ID NO: 692; SEQ ID NO: 693; SEQ ID NO: 694; SEQ ID NO: 695; SEQ ID NO: 696; SEQ ID NO: 697; SEQ ID NO: 698; SEQ ID NO: 699; SEQ ID NO: 700; SEQ ID NO: 701; SEQ ID NO: 702; SEQ ID NO: 703; SEQ ID NO: 704; SEQ ID NO: 705; SEQ ID NO: 706; SEQ ID NO: 707; SEQ ID NO: 708; SEQ ID NO: 709; SEQ ID NO: 710; SEQ ID NO: 711; SEQ ID NO: 712; SEQ ID NO: 713; SEQ ID NO: 714; SEQ ID NO: 715; SEQ ID NO: 716; SEQ ID NO: 717; SEQ ID NO: 718; SEQ ID NO: 719; SEQ ID NO: 720; SEQ ID NO: 721; SEQ ID NO: 722; SEQ ID NO: 723; SEQ ID NO: 724; SEQ ID NO: 725; SEQ ID NO: 726; SEQ ID NO: 727; SEQ ID NO: 728; SEQ ID NO: 729; SEQ ID NO: 730; SEQ ID NO: 731; SEQ ID NO: 732; SEQ ID NO: 733; SEQ ID NO: 734; SEQ ID NO: 735; SEQ ID NO: 736; SEQ ID NO: 737; SEQ ID NO: 738; SEQ ID NO: 739; SEQ ID NO: 740; SEQ ID NO: 741; SEQ ID NO: 742; SEQ ID NO: 743; SEQ ID NO: 744; SEQ ID NO: 745; SEQ ID NO: 746; SEQ ID NO: 747; SEQ ID NO: 748; SEQ ID NO: 749; SEQ ID NO: 750; SEQ ID NO: 751; SEQ ID NO: 752; SEQ ID NO: 753; SEQ ID NO: 754; SEQ ID NO: 755; SEQ ID NO: 756; SEQ ID NO: 757; SEQ ID NO: 758; SEQ ID NO: 759; SEQ ID NO: 760; SEQ ID NO: 761; SEQ ID NO: 762; SEQ ID NO: 763; SEQ ID NO: 764; SEQ ID NO: 765; SEQ ID NO: 766; SEQ ID NO: 767; SEQ ID NO: 768; SEQ ID NO: 769; SEQ ID NO: 770; SEQ ID NO: 771; SEQ ID NO: 772; SEQ ID NO: 773; SEQ ID NO: 774; SEQ ID NO: 775; SEQ ID NO: 776; SEQ ID NO: 777; SEQ ID NO: 778; SEQ ID NO: 779; SEQ ID NO: 780; SEQ ID NO: 781; SEQ ID NO: 782; SEQ ID NO: 783; SEQ ID NO: 784; SEQ ID NO: 785; SEQ ID NO: 786; SEQ ID NO: 787; SEQ ID NO: 788; SEQ ID NO: 789; SEQ ID NO: 790; SEQ ID NO: 791; SEQ ID NO: 792; SEQ ID NO: 793; SEQ ID NO: 794; SEQ ID NO: 795; SEQ ID NO: 796; SEQ ID NO: 797; SEQ ID NO: 798; SEQ ID NO: 799; SEQ ID NO: 800; SEQ ID NO: 801; SEQ ID NO: 802; SEQ ID NO: 803; SEQ ID NO: 804; SEQ ID NO: 805; SEQ ID NO: 806; SEQ ID NO: 807; SEQ ID NO: 808; SEQ ID NO: 809; SEQ ID NO: 810; SEQ ID NO: 811; SEQ ID NO: 812; SEQ ID NO: 813; SEQ ID NO: 814; SEQ ID NO: 815; SEQ ID NO: 816; SEQ ID NO: 817; SEQ ID NO: 818; SEQ ID NO: 819; SEQ ID NO: 820; SEQ ID NO: 821; SEQ ID NO: 822; SEQ ID NO: 823; SEQ ID NO: 824; SEQ ID NO: 825; SEQ ID NO: 826; SEQ ID NO: 827; SEQ ID NO: 828; SEQ ID NO: 829; SEQ ID NO: 830; SEQ ID NO: 831; SEQ ID NO: 832; SEQ ID NO: 833; SEQ ID NO: 834; SEQ ID NO: 835; SEQ ID NO: 836; SEQ ID NO: 837; SEQ ID NO: 838; SEQ ID NO: 839; SEQ ID NO: 840; SEQ ID NO: 841; SEQ ID NO: 842; SEQ ID NO: 843; SEQ ID NO: 844; SEQ ID NO: 845; SEQ ID NO: 846; SEQ ID NO: 847; SEQ ID NO: 848; SEQ ID NO: 849; SEQ ID NO: 850; SEQ ID NO: 851; SEQ ID NO: 852; SEQ ID NO: 853; SEQ ID NO: 854; SEQ ID NO: 855; SEQ ID NO: 856; SEQ ID NO: 857; SEQ ID NO: 858; SEQ ID NO: 859; SEQ ID NO: 860; SEQ ID NO: 861; SEQ ID NO: 862; SEQ ID NO: 863; SEQ ID NO: 864; SEQ ID NO: 865; SEQ ID NO: 866; SEQ ID NO: 867; SEQ ID NO: 868; SEQ ID NO: 869; SEQ ID NO: 870; SEQ ID NO: 871; SEQ ID NO: 872; SEQ ID NO: 873; SEQ ID NO: 874; SEQ ID NO: 875; SEQ ID NO: 876; SEQ ID NO: 877; SEQ ID NO: 878; SEQ ID NO: 879; SEQ ID NO: 880; SEQ ID NO: 881; SEQ ID NO: 882; SEQ ID NO: 883; SEQ ID NO: 884; SEQ ID NO: 885; SEQ ID NO: 886; SEQ ID NO: 887; SEQ ID NO: 888; SEQ ID NO: 889; SEQ ID NO: 890; SEQ ID NO: 891; SEQ ID NO: 892; SEQ ID NO: 893; SEQ ID NO: 894; SEQ ID NO: 895; SEQ ID NO: 896; SEQ ID NO: 897; SEQ ID NO: 898; SEQ ID NO: 899; SEQ ID NO: 900; SEQ ID NO: 901; SEQ ID NO: 902; SEQ ID NO: 903; SEQ ID NO: 904; SEQ ID NO: 905; SEQ ID NO: 906; SEQ ID NO: 907; SEQ ID NO: 908; SEQ ID NO: 909; SEQ ID NO: 910; SEQ ID NO: 911; SEQ ID NO: 912; SEQ ID NO: 913; SEQ ID NO: 914; SEQ ID NO: 915; SEQ ID NO: 916; SEQ ID NO: 917; SEQ ID NO: 918; SEQ ID NO: 919; SEQ ID NO: 920; SEQ ID NO: 921; SEQ ID NO: 922; SEQ ID NO: 923; SEQ ID NO: 924; SEQ ID NO: 925; SEQ ID NO: 926; SEQ ID NO: 927; SEQ ID NO: 928; SEQ ID NO: 929; SEQ ID NO: 930; SEQ ID NO: 931; SEQ ID NO: 932; SEQ ID NO: 933; SEQ ID NO: 934; SEQ ID NO: 935; SEQ ID NO: 936; SEQ ID NO: 937; SEQ ID NO: 938; SEQ ID NO: 939; SEQ, ID NO: 940; SEQ ID NO: 941; SEQ ID NO: 942; SEQ ID NO: 943; SEQ ID NO: 944; SEQ ID NO: 945; SEQ ID NO: 946; SEQ ID NO: 947; SEQ ID NO: 948; SEQ ID NO: 949; SEQ ID NO: 950; SEQ ID NO: 951; SEQ ID NO: 952; SEQ ID NO: 953; SEQ ID NO: 954; SEQ ID NO: 955; SEQ ID NO: 956; SEQ ID NO: 957; SEQ ID NO: 958; SEQ ID NO: 959; SEQ ID NO: 960; SEQ ID NO: 961; SEQ ID NO: 962; SEQ ID NO: 963; SEQ ID NO: 964; SEQ ID NO: 965; SEQ ID NO: 966; SEQ ID NO: 967; SEQ ID NO: 968; SEQ ID NO: 969; SEQ ID NO: 970; SEQ ID NO: 971; SEQ ID NO: 972; SEQ ID NO: 973; SEQ ID NO: 974; SEQ ID NO: 975; SEQ ID NO: 976; SEQ ID NO: 977; SEQ ID NO: 978; SEQ ID NO: 979; SEQ ID NO: 980; SEQ ID NO: 981; SEQ ID NO: 982; SEQ ID NO: 983; SEQ ID NO: 984; SEQ ID NO: 985; SEQ ID NO: 986; SEQ ID NO: 987; SEQ ID NO: 988; SEQ ID NO: 989; SEQ ID NO: 990; SEQ ID NO: 991; SEQ ID NO: 992; SEQ ID NO: 993; SEQ ID NO: 994; SEQ ID NO: 995; SEQ ID NO: 996; SEQ ID NO: 997; SEQ ID NO: 998; SEQ ID NO: 999; SEQ ID NO: 1000; SEQ ID NO: 1001; SEQ ID NO: 1002; SEQ ID NO: 1003; SEQ ID NO: 1004; SEQ ID NO: 1005; SEQ ID NO: 1006; SEQ ID NO: 1007; SEQ ID NO: 1008; SEQ ID NO: 1009; SEQ ID NO: 1010; SEQ ID NO: 1011; SEQ ID NO: 1012; SEQ ID NO: 1013; SEQ ID NO: 1014; SEQ ID NO: 1015; SEQ ID NO: 1016; SEQ ID NO: 10.7, SEQ ID NO: 1018; SEQ ID NO: 1019; SEQ ID NO: 1020; SEQ ID NO: 1021; SEQ ID NO: 1022; SEQ ID NO: 1023; SEQ ID NO: 1024; SEQ ID NO: 1025; SEQ ID NO: 1026; SEQ ID NO: 1027; SEQ ID NO: 1028; SEQ ID NO: 1029; SEQ ID NO: 1030; SEQ ID NO: 1031; SEQ ID NO: 1032; SEQ ID NO: 1033; SEQ ID NO: 1034; SEQ ID NO: 1035; SEQ ID NO: 1036; SEQ ID NO: 1037; SEQ ID NO: 1038; SEQ ID NO: 1039; SEQ ID NO: 1040; SEQ ID NO: 1041; SEQ ID NO: 1042; SEQ ID NO: 1043; SEQ ID NO: 1044; SEQ ID NO: 1045; SEQ ID NO: 1046; SEQ ID NO: 1047; SEQ ID NO: 1048; SEQ ID NO: 1049; SEQ ID NO: 1050; SEQ ID NO: 1051; SEQ ID NO: 1052; SEQ ID NO: 1053; SEQ ID NO: 1054; SEQ ID NO: 1055; SEQ ID NO: 1056; SEQ ID NO: 1057; SEQ ID NO: 1058; SEQ ID NO: 1059; SEQ ID NO: 1060; SEQ ID NO: 1061; SEQ ID NO: 1062; SEQ ID NO: 1063; SEQ ID NO: 1064; SEQ ID NO: 1065; SEQ ID NO: 1066; SEQ ID NO: 1067; SEQ ID NO: 1068; SEQ ID NO: 1069; SEQ ID NO: 1070; SEQ ID NO: 1071; SEQ ID NO: 1072; SEQ ID NO: 1073; SEQ ID NO: 1074; SEQ ID NO: 1075; SEQ ID NO: 1076; SEQ ID NO: 1077; SEQ ID NO: 1078; SEQ ID NO: 1079; SEQ ID NO: 1080; SEQ ID NO: 1081; SEQ ID NO: 1082; SEQ ID NO: 1083; SEQ ID NO: 1084; SEQ ID NO: 1085; SEQ ID NO: 1086; SEQ ID NO: 1087; SEQ ID NO: 1088; SEQ ID NO: 1089; SEQ ID NO: 1090; SEQ ID NO: 1091; SEQ ID NO: 1092; SEQ ID NO: 1093; SEQ ID NO: 1094; SEQ ID NO: 1095; SEQ ID NO: 1096; SEQ ID NO: 1097; SEQ ID NO: 1098; SEQ ID NO: 1099; SEQ ID NO: 1100; SEQ ID NO: 1101; SEQ ID NO: 1102; SEQ ID NO: 1103; SEQ ID NO: 1104; SEQ ID NO: 1105; SEQ ID NO: 1106; SEQ ID NO: 1107; SEQ ID NO: 1108; SEQ ID NO: 1109; SEQ ID NO: 1110; SEQ ID NO: 1111; SEQ ID NO: 1112; SEQ ID NO: 1113; SEQ ID NO: 1114; SEQ ID NO: 1115; SEQ ID NO: 1116; SEQ ID NO: 1117; SEQ ID NO: 1118; SEQ ID NO: 1119; SEQ ID NO: 1120; SEQ ID NO: 1121; SEQ ID NO: 1122; SEQ ID NO: 1123; SEQ ID NO: 1124; SEQ ID NO: 1125; SEQ ID NO: 1126; SEQ ID NO: 1127; SEQ ID NO: 1128; SEQ ID NO: 1129; SEQ ID NO: 1130; SEQ ID NO: 1131; SEQ ID NO: 1132; SEQ ID NO: 1133; SEQ ID NO: 1134; SEQ ID NO: 1135; SEQ ID NO: 1136; SEQ ID NO: 1137; SEQ ID NO: 1138; SEQ ID NO: 1139; SEQ ID NO: 1140; SEQ ID NO: 1141; SEQ ID NO: 1142; SEQ ID NO: 1143; SEQ ID NO: 1144; SEQ ID NO: 1145; SEQ ID NO: 1146; SEQ ID NO: 1147; SEQ ID NO: 1148; SEQ ID NO: 1149; SEQ ID NO: 1150; SEQ ID NO: 1151; SEQ ID NO: 1152; SEQ ID NO: 1153; SEQ ID NO: 1154; SEQ ID NO: 1155; SEQ ID NO: 1156; SEQ ID NO: 1157; SEQ ID NO: 1158; SEQ ID NO: 1159; SEQ ID NO: 1160; SEQ ID NO: 1161; SEQ ID NO: 1162; SEQ ID NO: 1163; SEQ ID NO: 1164; SEQ ID NO: 1165; SEQ ID NO: 1166; SEQ ID NO: 1167; SEQ ID NO: 1168; SEQ ID NO: 1169; SEQ ID NO:1170; SEQ ID NO:1171; SEQ ID NO:1172; SEQ ID NO: 1173; SEQ ID NO: 1174; SEQ ID NO: 1175; SEQ ID NO: 1176; SEQ ID NO: 1177; SEQ ID NO: 1178; SEQ ID NO: 1179; SEQ ID NO: 1180; SEQ ID NO:1181; SEQ ID NO: 1182; SEQ ID NO: 1183; SEQ ID NO: 1184; SEQ ID NO: 1185; SEQ ID NO: 1&#39;186; SEQ ID NO: 1187; SEQ ID NO: 1188; SEQ ID NO: 1189; SEQ ID NO: 1190; SEQ ID NO: 1191; SEQ ID NO: 1192; SEQ ID NO: 1193; SEQ ID NO: 1194; SEQ ID NO: 1195; SEQ ID NO: 1196; SEQ ID NO: 1197; SEQ ID NO: 1198; SEQ ID NO: 1199; SEQ ID NO: 1200; SEQ ID NO: 1201; SEQ ID NO: 1202; SEQ ID NO: 1203; SEQ ID NO: 1204; SEQ ID NO: 1205; SEQ ID NO: 1206; SEQ ID NO: 1207; SEQ ID NO: 1208; SEQ ID NO: 1209; SEQ ID NO: 1210; SEQ ID NO: 1121; SEQ ID NO: 1212; SEQ ID NO:1213; SEQ ID NO:1214; SEQ ID NO: 1215; SEQ ID NO:1216; SEQ ID NO: 1217; SEQ ID NO: 1218; SEQ ID NO: 1219; SEQ ID NO: 1220; SEQ ID NO: 1221; SEQ ID NO: 1222; SEQ ID NO: 1223; SEQ ID NO: 1224; SEQ ID NO: 1225; SEQ ID NO: 1226; SEQ ID NO: 1227; SEQ ID NO: 1228; SEQ ID NO: 1229; SEQ ID NO: 1230; SEQ ID NO: 1231; SEQ ID NO: 1232; SEQ ID NO: 1233; SEQ ID NO: 1234; SEQ ID NO: 1235; SEQ ID NO: 1236; SEQ ID NO: 1237; SEQ ID NO: 1238; SEQ ID NO: 1239; SEQ ID NO: 1240; SEQ ID NO: 1241; SEQ ID NO: 1242; SEQ ID NO: 1243; SEQ ID NO: 1244; SEQ ID NO: 1245; SEQ ID NO: 1246; SEQ ID NO: 1247; SEQ ID NO: 1248; SEQ ID NO: 1249; SEQ ID NO: 1250; SEQ ID NO: 1251; SEQ ID NO: 1252; SEQ ID NO: 1253; SEQ ID NO: 1254; SEQ ID NO: 1255; SEQ ID NO: 1256; SEQ ID NO: 1257; SEQ ID NO: 1258; SEQ ID NO: 1259; SEQ ID NO: 1260; SEQ ID NO: 1261; SEQ ID NO: 1262; SEQ ID NO: 1263; SEQ ID NO: 1264; SEQ ID NO: 1265; SEQ ID NO: 1266; SEQ ID NO: 1267; SEQ ID NO: 1268; SEQ ID NO: 1269; SEQ ID NO: 1270; SEQ ID NO: 1271; SEQ ID NO: 1272; SEQ ID NO: 1273; SEQ ID NO: 1274; SEQ ID NO: 1275; SEQ ID NO: 1276; SEQ ID NO: 1277; SEQ ID NO: 1278; SEQ ID NO: 1279; SEQ ID NO: 1280; SEQ ID NO: 1281; SEQ ID NO: 1282; SEQ ID NO: 1283; SEQ ID NO: 1284; SEQ ID NO: 1285; SEQ ID NO: 1286; SEQ ID NO: 1287; SEQ ID NO: 1288; SEQ ID NO: 1289; SEQ ID NO: 1290; SEQ ID NO: 1291; SEQ ID NO: 1292; SEQ ID NO: 1293; SEQ ID NO: 1294; SEQ ID NO: 1295; SEQ ID NO: 1296; SEQ ID NO: 1297; SEQ ID NO: 1298; SEQ ID NO: 1299; SEQ ID NO: 1300; SEQ ID NO: 1301; SEQ ID NO: 1302; SEQ ID NO: 1303; SEQ ID NO: 1304; SEQ ID NO: 1305; SEQ ID NO: 1306; SEQ ID NO: 1307; SEQ ID NO: 1308; SEQ ID NO: 1309; SEQ ID NO: 1310; SEQ ID NO: 1311; SEQ ID NO:1312; SEQ ID NO: 1313; SEQ ID NO:1314; SEQ ID NO:1315; SEQ ID NO: 1316; SEQ ID NO: 1317; SEQ ID NO: 1318; SEQ ID NO: 1319; SEQ ID NO: 1320; SEQ ID NO: 1321; SEQ ID NO: 1322; SEQ ID NO: 1323; SEQ ID NO: 1324; SEQ ID NO: 1325; SEQ ID NO: 1326; SEQ ID NO: 1327; SEQ ID NO: 1328; SEQ ID NO: 1329; SEQ ID NO: 1330; SEQ ID NO: 1331; SEQ ID NO: 1332; SEQ ID NO: 1333; SEQ ID NO: 1334; SEQ ID NO: 1335; SEQ ID NO: 1336; SEQ ID NO: 1337; SEQ ID NO: 1338; SEQ ID NO: 1339; SEQ ID NO: 1340; SEQ ID NO: 1341; SEQ ID NO: 1342; SEQ ID NO: 1343; SEQ ID NO: 1344; SEQ ID NO: 1345; SEQ ID NO: 1346; SEQ ID NO: 1347; SEQ ID NO: 1348; SEQ ID NO: 1349; SEQ ID NO: 1350; SEQ ID NO: 1351; SEQ ID NO: 1352; SEQ ID NO: 1353; SEQ ID NO: 1354; SEQ ID NO: 1355; SEQ ID NO: 1356; SEQ ID NO: 1357; SEQ ID NO: 1358; SEQ ID NO: 1359; SEQ ID NO: 1360; SEQ ID NO: 1361; SEQ ID NO: 1362; SEQ ID NO: 1363; SEQ ID NO: 1364; SEQ ID NO: 1365; SEQ ID NO: 1366; SEQ ID NO: 1367; SEQ ID NO: 1368; SEQ ID NO: 1369; SEQ ID NO: 1370; SEQ ID NO: 1371; SEQ ID NO: 1372; SEQ ID NO: 1373; SEQ ID NO: 1374; SEQ ID NO: 1375; SEQ ID NO: 1376; SEQ ID NO: 1377; SEQ ID NO: 1378; SEQ ID NO: 1379; SEQ ID NO: 1380; SEQ ID NO: 1381; SEQ ID NO: 1382; SEQ ID NO: 1383; SEQ ID NO: 1384; SEQ ID NO: 1385; SEQ ID NO: 1386; SEQ ID NO: 1387; SEQ ID NO: 1388; SEQ ID NO: 1389; SEQ ID NO: 1390; SEQ ID NO: 1391; SEQ ID NO: 1392; SEQ ID NO: 1393; SEQ ID NO: 1394; SEQ ID NO: 1395; SEQ ID NO: 1396; SEQ ID NO: 1397; SEQ ID NO: 1398; SEQ ID NO: 1399; SEQ ID NO: 1400; SEQ ID NO: 1401; SEQ ID NO: 1402; SEQ ID NO: 1403; SEQ ID NO: 1404; SEQ ID NO: 1405; SEQ ID NO: 1406; SEQ ID NO: 1407; SEQ ID NO: 1408; SEQ ID NO: 1409; SEQ ID NO: 1410; SEQ ID NO: 1411; SEQ ID NO: 1412; SEQ ID NO: 1413; SEQ ID NO: 1414; SEQ ID NO: 1415; SEQ ID NO: 1416; SEQ ID NO: 1417; SEQ ID NO: 1418; SEQ ID NO: 1419; SEQ ID NO: 1420; SEQ ID NO: 1421; SEQ ID NO: 1422; SEQ ID NO: 1423; SEQ ID NO: 1424; SEQ ID NO: 1425; SEQ ID NO: 1426; SEQ ID NO: 1427; SEQ ID NO: 1428; SEQ ID NO: 1429; SEQ ID NO: 1430; SEQ ID NO: 1431; SEQ ID NO: 1432; SEQ ID NO: 1433; SEQ ID NO: 1434; SEQ ID NO: 1435; SEQ ID NO: 1436; SEQ ID NO: 1437; SEQ ID NO: 1438; SEQ ID NO: 1439; SEQ ID NO: 1440; SEQ ID NO: 1441; SEQ ID NO: 1442; SEQ ID NO: 1443; SEQ ID NO: 1444; SEQ ID NO: 1445; SEQ ID NO: 1446; SEQ ID NO: 1447; SEQ ID NO: 1448; SEQ ID NO: 1449; SEQ ID NO: 1450; SEQ ID NO: 1451; SEQ ID NO: 1452; SEQ ID NO: 1453; SEQ ID NO: 1454; SEQ ID NO: 1455; SEQ ID NO: 1456; SEQ ID NO: 1457; SEQ ID NO: 1458; SEQ ID NO: 1459; SEQ ID NO: 1460; SEQ ID NO: 1461; SEQ ID NO: 1462; SEQ ID NO: 1463; SEQ ID NO: 1464; SEQ ID NO: 1465; SEQ ID NO: 1466; SEQ ID NO: 1467; SEQ ID NO: 1468; SEQ ID NO: 1469; SEQ ID NO: 1470; SEQ ID NO: 1471; SEQ ID NO: 1472; SEQ ID NO: 1473; SEQ ID NO: 1474; SEQ ID NO: 1475; SEQ ID NO: 1476; SEQ ID NO: 1477; SEQ ID NO: 1478; SEQ ID NO: 1479; SEQ ID NO: 1480; SEQ ID NO: 1481; SEQ ID NO: 1482; SEQ ID NO: 1483; SEQ ID NO: 1484; SEQ ID NO: 1485; SEQ ID NO: 1486; SEQ ID NO: 1487; SEQ ID NO: 1488; SEQ ID NO: 1489; SEQ ID NO: 1490; SEQ ID NO: 1491; SEQ ID NO: 1492; SEQ ID NO: 1493; SEQ ID NO: 1494; SEQ ID NO: 1495; SEQ ID NO: 1496; SEQ ID NO: 1497; SEQ ID NO: 1498; SEQ ID NO: 1499; SEQ ID NO: 1500; SEQ ID NO: 1501; SEQ ID NO: 1502; SEQ ID NO: 1503; SEQ ID NO: 1504; SEQ ID NO: 1505; SEQ ID NO: 1506; SEQ ID NO: 1507; SEQ ID NO: 1508; SEQ ID NO: 1509; SEQ ID NO: 1510; SEQ ID NO: 1511; SEQ ID NO: 1512, S2Q ID NO: 1513; SEQ ID NO: 1514; SEQ ID NO: 1515; SEQ ID NO: 1516; SEQ ID NO: 1517; SEQ ID NO: 1518; SEQ ID NO: 1519; SEQ ID NO: 1520; SEQ ID NO: 1521; SEQ ID NO: 1522; SEQ ID NO: 1523; SEQ ID NO: 1524; SEQ ID NO: 1525; SEQ ID NO: 1526; SEQ ID NO: 1527; SEQ ID NO: 1528; SEQ ID NO: 1529; SEQ ID NO: 1530; SEQ ID NO: 1531; SEQ ID NO: 1532; SEQ ID NO: 1533; SEQ ID NO: 1534; SEQ ID NO: 1535; SEQ ID NO: 1536; SEQ ID NO: 1537; SEQ ID NO: 1538; SEQ ID NO: 1539; SEQ ID NO: 1540; SEQ ID NO: 1541; SEQ ID NO: 1542; SEQ ID NO: 1543; SEQ ID NO: 1544; SEQ ID NO: 1545; SEQ ID NO: 1546; SEQ ID NO: 1547; SEQ ID NO: 1548; SEQ ID NO: 1549; SEQ ID NO: 1550; SEQ ID NO: 1551; SEQ ID NO: 1552; SEQ ID NO: 1553; SEQ ID NO: 1554; SEQ ID NO: 1555; SEQ ID NO: 1556; SEQ ID NO: 1557; SEQ ID NO: 1558; SEQ ID NO: 1559; SEQ ID NO: 1560; SEQ ID NO: 1561; SEQ ID NO: 1562; SEQ ID NO: 1563; SEQ ID NO: 1564; SEQ ID NO: 1565; SEQ ID NO: 1566; SEQ ID NO: 1567; SEQ ID NO: 1568; SEQ ID NO: 1569; SEQ ID NO: 1570; SEQ ID NO: 1571; SEQ ID NO: 1572; SEQ ID NO: 1573; SEQ ID NO: 1574; SEQ ID NO: 1575; and SEQ ID NO: 9525; SEQ ID NO: 9526; SEQ ID NO: 9527; SEQ ID NO: 9528; SEQ ID NO: 9529; SEQ ID NO: 9530; SEQ ID NO: 9531; SEQ ID NO: 9532; SEQ ID NO: 9533; SEQ ID NO: 9534; SEQ ID NO: 9535; SEQ ID NO: 9536; SEQ ID NO: 9537; SEQ ID NO: 9538; SEQ ID NO: 9539; SEQ ID NO: 9540; SEQ ID NO: 9541; SEQ ID NO: 9542; SEQ ID NO: 9543; SEQ ID NO: 9544; SEQ ID NO: 9545; SEQ ID NO: 9546; SEQ ID NO: 9547; SEQ ID NO: 9548; SEQ ID NO: 9549; SEQ ID NO: 9550; SEQ ID NO: 9551; SEQ ID NO: 9552; SEQ ID NO: 9553; SEQ ID NO: 9554; SEQ ID NO: 9555; SEQ ID NO: 9556; SEQ ID NO: 9557; SEQ ID NO: 9558; SEQ ID NO: 9559; SEQ ID NO: 9560; SEQ ID NO: 9561; SEQ ID NO: 9562; SEQ ID NO: 9563; SEQ ID NO: 9564; SEQ ID NO: 9565; SEQ ID NO: 9566; SEQ ID NO: 9567; SEQ ID NO: 9568; SEQ ID NO: 9569; SEQ ID NO: 9570; SEQ ID NO: 9571; SEQ ID NO: 9572; SEQ ID NO: 9573; SEQ ID NO: 9574; SEQ ID NO: 9575; SEQ ID NO: 9576; SEQ ID NO: 9577; SEQ ID NO: 9578; SEQ ID NO: 9579; SEQ ID NO: 9580; SEQ ID NO: 9581; SEQ ID NO: 9582; SEQ ID NO: 9583; SEQ ID NO: 9584; SEQ ID NO: 9585; SEQ ID NO: 9586; SEQ ID NO: 9587; SEQ ID NO: 9588; SEQ ID NO: 9589; SEQ ID NO: 9590; SEQ ID NO: 9591; SEQ ID NO:9592, or a complement thereof.  
     [0057] In one embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  flagella-associated polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1; SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 5; SEQ ID NO: 6; SEQ ID NO: 7; SEQ ID NO: 8; SEQ ID NO: 9; SEQ ID NO: 10; SEQ ID NO:11; SEQ ID NO: 12; SEQ ID NO: 13; SEQ ID NO: 14; SEQ ID NO: 15; SEQ ID NO: 16; SEQ ID NO: 17; SEQ ID NO: 18; SEQ ID NO: 19; SEQ ID NO: 20; SEQ ID NO: 21; SEQ ID NO: 22; SEQ ID NO: 23; SEQ ID NO: 24; SEQ ID NO: 25; SEQ ID NO: 26; SEQ ID NO: 27; SEQ ID NO: 28; SEQ ID NO: 29; SEQ ID NO: 30; SEQ ID NO: 31; SEQ ID NO: 32; SEQ ID NO: 33; SEQ ID NO: 34; SEQ ID NO: 35; SEQ ID NO: 36; SEQ ID NO: 37; SEQ ID NO: 38; SEQ ID NO: 39; SEQ ID NO: 40; SEQ ID NO: 41; SEQ ID NO: 42; SEQ ID NO: 43; SEQ ID NO: 44; SEQ ID NO: 45; SEQ ID NO: 46; SEQ ID NO: 47; SEQ ID NO: 48; SEQ ID NO: 49; SEQ ID NO: 50; SEQ ID NO: 51; SEQ ID NO: 52; SEQ ID NO: 53; SEQ ID NO: 54; SEQ ID NO: 55; SEQ ID NO: 56; SEQ ID NO: 57; SEQ ID NO: 58; SEQ ID NO: 59; SEQ ID NO: 60; SEQ ID NO: 61; SEQ ID NO: 62; SEQ ID NO: 63; SEQ ID NO: 64; SEQ ID NO: 65; SEQ ID NO: 66; SEQ ID NO: 67; SEQ ID NO: 68; SEQ ID NO: 69; SEQ ID NO: 70; SEQ ID NO: 71; SEQ ID NO: 72; SEQ ID NO: 73; SEQ ID NO: 74; SEQ ID NO: 75; SEQ ID NO: 76; SEQ ID NO: 77; SEQ ID NO: 78; SEQ ID NO: 79; SEQ ID NO: 80; SEQ ID NO: 81; SEQ ID NO: 82; SEQ ID NO: 83; SEQ ID NO: 84; SEQ ID NO: 85; SEQ ID NO: 86; SEQ ID NO: 87; SEQ ID NO: 88; SEQ ID NO: 89; SEQ ID NO: 90; SEQ ID NO: 91; SEQ ID NO: 92; SEQ ID NO: 93; SEQ ID NO: 94; SEQ ID NO: 95; SEQ ID NO: 96; SEQ ID NO: 97; SEQ ID NO: 98; SEQ ID NO: 99, SEQ ID NO: 100; SEQ ID NO: 101; SEQ ID NO: 102; SEQ ID NO: 103; and SEQ ID NO: 9525; SEQ ID NO: 9526; SEQ ID NO: 9527, or a complement thereof. In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  outer membrane polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 104; SEQ ID NO: 105; SEQ ID NO: 106; SEQ ID NO: 107; SEQ ID NO: 108; SEQ ID NO: 109; SEQ ID NO: 110; SEQ ID NO: 111; SEQ ID NO: 112; SEQ ID NO: 113; SEQ ID NO: 114; SEQ ID NO: 115; SEQ ID NO: 116; SEQ ID NO: 117; SEQ ID NO: 118; SEQ ID NO: 119; SEQ ID NO: 120; SEQ ID NO: 121; SEQ ID NO: 122; SEQ ID NO: 123; SEQ ID NO: 124; SEQ ID NO: 125; SEQ ID NO: 126; SEQ ID NO: 127; SEQ ID NO: 128; SEQ ID NO: 129; SEQ ID NO: 130; SEQ ID NO: 131; SEQ ID NO: 132; SEQ ID NO: 133; SEQ ID NO: 134; SEQ ID NO: 135; SEQ ID NO: 136; SEQ ID NO: 137; SEQ ID NO: 138; SEQ ID NO: 139; SEQ ID NO: 140; SEQ ID NO: 141; SEQ ID NO: 142; SEQ ID NO: 143; SEQ ID NO: 144; SEQ ID NO: 145; SEQ ID NO: 146; SEQ ID NO: 147; SEQ ID NO: 148; SEQ ID NO: 149; SEQ ID NO: 150; SEQ ID NO: 151; SEQ ID NO: 152; SEQ ID NO: 153; SEQ ID NO: 154; SEQ ID NO: 155; SEQ ID NO: 156; SEQ ID NO: 157; SEQ ID NO: 158; SEQ ID NO: 159; SEQ ID NO: 160; SEQ ID NO: 161; SEQ ID NO: 162; SEQ ID NO: 163; SEQ ID NO: 164; SEQ ID NO: 165; SEQ ID NO: 166; SEQ ID NO: 167; SEQ ID NO: 168; SEQ ID NO: 169; SEQ ID NO: 170; SEQ ID NO: 171; SEQ ID NO: 172; SEQ ID NO: 173; SEQ ID NO: 174; SEQ ID NO: 175; SEQ ID NO: 176; SEQ ID NO: 177; SEQ ID NO: 178; SEQ ID NO: 179; SEQ ID NO: 180; SEQ ID NO: 181; SEQ ID NO: 182; SEQ ID NO: 183; SEQ ID NO: 184; SEQ ID NO: 185; SEQ ID NO: 186; SEQ ID NO: 187, SEQ ID NO: 188; SEQ ID NO: 189; SEQ ID NO: 190; SEQ ID NO: 191; SEQ ID NO: 192; SEQ ID NO: 193; SEQ ID NO: 194; SEQ ID NO: 195; SEQ ID NO: 196; SEQ ID NO: 197; SEQ ID NO: 198; SEQ ID NO: 199; SEQ ID NO: 200; SEQ ID NO: 201; SEQ ID NO: 202; SEQ ID NO: 203; SEQ ID NO: 204; SEQ ID NO: 205; SEQ ID NO: 206; SEQ ID NO: 207; SEQ ID NO: 208; SEQ ID NO: 209; SEQ ID NO: 210; SEQ ID NO: 211; SEQ ID NO: 212; SEQ ID NO: 213; SEQ ID NO: 214; SEQ ID NO: 215; SEQ ID NO: 216; SEQ ID NO: 217; SEQ ID NO: 218; SEQ ID NO: 219; SEQ ID NO: 220; SEQ ID NO: 221; SEQ ID NO: 222; SEQ ID NO: 223; SEQ ID NO: 224; SEQ ID NO: 225; SEQ ID NO: 226; SEQ ID NO: 227; SEQ ID NO: 228; SEQ ID NO: 229; SEQ ID NO: 230; SEQ ID NO: 231; SEQ ID NO: 232; SEQ ID NO: 233; SEQ ID NO: 234; SEQ ID NO: 235; SEQ ID NO: 236; SEQ ID NO: 237; SEQ ID NO: 238; SEQ ID NO: 239; SEQ ID NO: 240; SEQ ID NO: 241; SEQ ID NO: 242; SEQ ID NO: 243; SEQ ID NO: 244; SEQ ID NO: 245; SEQ ID NO: 246; SEQ ID NO: 247; SEQ ID NO: 248; SEQ ID NO: 249; SEQ ID NO: 250; SEQ ID NO: 251; SEQ ID NO: 252; SEQ ID NO: 253; SEQ ID NO: 254; SEQ ID NO: 255; SEQ ID NO: 256; SEQ ID NO: 257; SEQ ID NO: 258; SEQ ID NO: 259; SEQ ID NO: 260; SEQ ID NO: 261; SEQ ID NO: 262; SEQ ID NO: 263; SEQ ID NO: 264; SEQ ID NO: 265; SEQ ID NO: 266; SEQ ID NO: 267; SEQ ID NO: 268; SEQ ID NO: 269; SEQ ID NO: 270; SEQ ID NO: 271; SEQ ID NO: 272; SEQ ID NO: 273; SEQ ID NO: 274; SEQ ID NO: 275; SEQ ID NO: 276; SEQ ID NO: 277; SEQ ID NO: 278; SEQ ID NO: 279; SEQ ID NO: 280; SEQ ID NO: 281; SEQ ID NO: 282; SEQ ID NO: 283; SEQ ID NO: 284; SEQ ID NO: 285; SEQ ID NO: 286; SEQ ID NO: 287; SEQ ID NO: 288; SEQ ID NO: 289; SEQ ID NO: 290; SEQ ID NO: 291; SEQ ID NO: 292; SEQ ID NO: 293; SEQ ID NO: 294; SEQ ID NO: 295; SEQ ID NO: 296; SEQ ID NO: 297; SEQ ID NO: 298; SEQ ID NO: 299; SEQ ID NO: 300; SEQ ID NO: 301; SEQ ID NO: 302; SEQ ID NO: 303; SEQ ID NO: 304; SEQ ID NO: 305; SEQ ID NO: 306; SEQ ID NO: 307; SEQ ID NO: 308; SEQ ID NO: 309; SEQ ID NO: 310; SEQ ID NO: 311; SEQ ID NO: 312; SEQ ID NO: 313; SEQ ID NO: 314; SEQ ID NO: 315; SEQ ID NO: 316; SEQ ID NO: 317; SEQ ID NO: 318; SEQ ID NO: 319; SEQ ID NO: 320; SEQ ID NO: 321; SEQ ID NO: 322; SEQ ID NO: 323; SEQ ID NO: 324; SEQ ID NO: 325; SEQ ID NO: 326; SEQ ID NO: 327; SEQ ID NO: 328; SEQ ID NO: 329; SEQ ID NO: 330; SEQ ID NO: 331; SEQ ID NO: 332; SEQ ID NO: 333; SEQ ID NO: 334; SEQ ID NO: 335; SEQ ID NO: 336; SEQ ID NO: 337; SEQ ID NO: 338; SEQ ID NO: 339; SEQ ID NO: 340; SEQ ID NO: 341; SEQ ID NO: 342; SEQ ID NO: 343; SEQ ID NO: 344; SEQ ID NO: 345; SEQ ID NO: 346; SEQ ID NO: 347; SEQ ID NO: 348; SEQ ID NO: 349; SEQ ID NO: 350; SEQ ID NO: 351; SEQ ID NO: 352; SEQ ID NO: 353; SEQ ID NO: 354; SEQ ID NO: 355; SEQ ID NO: 356; SEQ ID NO: 357; SEQ ID NO: 358; SEQ ID NO: 359; SEQ ID NO: 360; SEQ ID NO: 361; SEQ ID NO: 362; SEQ ID ND. 363; SEQ ID NO: 364; SEQ ID NO: 365; SEQ ID NO: 366; SEQ ID NO: 367; SEQ ID NO: 368; SEQ ID NO: 369; SEQ ID NO: 370; SEQ ID NO: 371; SEQ ID NO: 372; SEQ ID NO: 373; SEQ ID NO: 374; SEQ ID NO: 375; SEQ ID NO: 376; SEQ ID NO: 377; SEQ ID NO: 378; SEQ ID NO: 379; SEQ ID NO: 380; SEQ ID NO: 381; SEQ ID NO: 382; SEQ ID NO: 383; SEQ ID NO: 384; SEQ ID NO: 385; SEQ ID NO: 386; SEQ ID NO: 387; SEQ ID NO: 388; SEQ ID NO: 389; SEQ ID NO: 390; SEQ ID NO: 391; SEQ ID NO: 392; SEQ ID NO: 393; SEQ ID NO: 394; SEQ ID NO: 395; SEQ ID NO: 396; SEQ ID NO: 397; SEQ ID NO: 398; SEQ ID NO: 399; SEQ ID NO: 400; SEQ ID NO: 401; SEQ ID NO: 402; SEQ ID NO: 403; SEQ ID NO: 404; SEQ ID NO: 405; SEQ ID NO: 406; SEQ ID NO: 407; SEQ ID NO: 408; SEQ ID NO: 409; SEQ ID NO: 410; SEQ ID NO: 411; SEQ ID NO: 412; SEQ ID NO: 413; SEQ ID NO: 414; SEQ ID NO: 415; SEQ ID NO: 416; SEQ ID NO: 417; SEQ ID NO: 418; SEQ ID NO: 419; SEQ ID NO: 420; SEQ ID NO: 421; SEQ ID NO: 422; SEQ ID NO: 423; SEQ ID NO: 424; SEQ ID NO: 425; SEQ ID NO: 426; SEQ ID NO: 427; SEQ ID NO: 428; SEQ ID NO: 429; SEQ ID NO: 430; SEQ ID NO: 431; SEQ ID NO: 432; SEQ ID NO: 433; SEQ ID NO: 434; SEQ ID NO: 435; SEQ ID NO: 436; SEQ ID NO: 437; SEQ ID NO: 438; SEQ ID NO: 439; SEQ ID NO: 440; SEQ ID NO: 441; SEQ ID NO: 442; SEQ ID NO: 443; SEQ ID NO: 444; SEQ ID NO: 445; SEQ ID NO: 446; SEQ ID NO: 447; SEQ ID NO: 448; SEQ ID NO: 449; SEQ ID NO: 450; SEQ ID NO: 451; SEQ ID NO: 452; SEQ ID NO: 453; SEQ ID NO: 454; SEQ ID NO: 455; SEQ ID NO: 456; SEQ ID NO: 457; SEQ ID NO: 458; SEQ ID NO: 459; SEQ ID NO: 460; SEQ ID NO: 461; SEQ ID NO: 462; SEQ ID NO: 463; SEQ ID NO: 464; SEQ ID NO: 465; SEQ ID NO: 466; SEQ ID NO: 467; SEQ ID NO: 468; SEQ ID NO: 469; SEQ ID NO: 470; SEQ ID NO: 471; SEQ ID NO: 472; SEQ ID NO: 473; SEQ ID NO: 474; SEQ ID NO: 475; SEQ ID NO: 476; SEQ ID NO: 477; SEQ ID NO: 478; SEQ ID NO: 479; SEQ ID NO: 480; SEQ ID NO: 481; SEQ ID NO: 482; SEQ ID NO: 483; SEQ ID NO: 484; SEQ ID NO: 485; SEQ ID NO: 486; SEQ ID NO: 487; SEQ ID NO: 488; SEQ ID NO: 489; SEQ ID NO: 490; SEQ ID NO: 491; SEQ ID NO: 492; SEQ ID NO: 493; SEQ ID NO: 494; SEQ ID NO: 495; SEQ ID NO: 496; SEQ ID NO: 497; SEQ ID NO: 498; SEQ ID NO: 499; SEQ ID NO: 500; SEQ ID NO: 501; SEQ ID NO: 502; SEQ ID NO: 503; SEQ ID NO: 504; SEQ ID NO: 505; SEQ ID NO: 506; SEQ ID NO: 507; SEQ ID NO: 508; SEQ ID NO: 509; SEQ ID NO: 510; and SEQ ID NO: 9528; SEQ ID NO: 9529; SEQ ID NO: 9530; SEQ ID NO: 9531; SEQ ID NO: 9532; SEQ ID NO: 9533; SEQ ID NO: 9534; SEQ ID NO: 9535; SEQ ID NO: 9536, or a complement thereof.  
     [0058] In another embodiment, the  H. pylori  outer membrane polypeptide or a fragment thereof is an  H. pylori  polypeptide having a terminal phenylalanine residue or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 104; SEQ ID NO: 105; SEQ ID NO: 106; SEQ ID NO: 107; SEQ ID NO: 108; SEQ ID NO: 109; SEQ ID NO: 110; SEQ ID NO: 111; SEQ ID NO: 112; SEQ ID NO: 113; SEQ ID NO: 114; SEQ ID NO: 115; SEQ ID NO: 116; SEQ ID NO: 117; SEQ ID NO: 118; SEQ ID NO: 119; SEQ ID NO: 120; SEQ ID NO: 121; SEQ ID NO: 122; SEQ ID NO: 123; SEQ ID NO: 124; SEQ ID NO: 125; SEQ ID NO: 126; SEQ ID NO: 127; SEQ ID NO: 128; SEQ ID NO: 129; SEQ ID NO: 130; SEQ ID NO: 131; SEQ ID NO: 132; SEQ ID NO: 133; SEQ ID NO: 134; SEQ ID NO: 135; SEQ ID NO: 136; SEQ ID NO: 137; SEQ ID NO: 138; SEQ ID NO: 139; SEQ ID NO: 140; SEQ ID NO: 141; SEQ ID NO: 142; SEQ ID NO: 143; SEQ ID NO: 144; SEQ ID NO: 145; SEQ ID NO: 146; SEQ ID NO: 147; SEQ ID NO: 148; SEQ ID NO: 149; SEQ ID NO: 150; SEQ ID NO: 151; SEQ ID NO: 152; SEQ ID NO: 153; SEQ ID NO: 154; SEQ ID NO: 155; SEQ ID NO: 156; SEQ ID NO: 157; SEQ ID NO: 158; SEQ ID NO: 159; SEQ ID NO: 160; SEQ ID NO: 161; SEQ ID NO: 162; SEQ ID NO: 163; SEQ ID NO: 164; SEQ ID NO: 165; SEQ ID NO: 166; SEQ ID NO: 167; SEQ ID NO: 168; SEQ ID NO: 169; SEQ ID NO: 170; SEQ ID NO: 171; SEQ ID NO: 172; SEQ ID NO: 173; SEQ ID NO: 174; SEQ ID NO: 175; SEQ ID NO: 176; SEQ ID NO: 177; SEQ ID NO: 178; SEQ ID NO: 179; SEQ ID NO: 180; SEQ ID NO: 181; SEQ ID NO: 182; SEQ ID NO: 183; SEQ ID NO: 184; SEQ ID NO: 185; SEQ ID NO: 186; SEQ ID NO: 187; SEQ ID NO: 188; SEQ ID NO: 189; SEQ ID NO: 190; SEQ ID NO: 191; SEQ ID NO: 192; SEQ ID NO: 193; SEQ ID NO: 194; SEQ ID NO: 195; SEQ ID NO: 196; SEQ ID NO: 197; SEQ ID NO: 198; SEQ ID NO: 199; SEQ ID NO: 200; SEQ ID NO: 201; SEQ ID NO: 202; SEQ ID NO: 203; SEQ ID NO: 204; SEQ ID NO: 205; SEQ ID NO: 206; SEQ ID NO: 207; SEQ ID NO: 208; SEQ ID NO: 209; SEQ ID NO: 210; SEQ ID NO: 211; SEQ ID NO: 212; SEQ ID NO: 213; SEQ ID NO: 214; SEQ ID NO: 215; SEQ ID NO: 216; SEQ ID NO: 217; SEQ ID NO: 218; SEQ ID NO: 219; SEQ ID NO: 220; SEQ ID NO: 221; SEQ ID NO: 222; SEQ ID NO: 223; SEQ ID NO: 224; SEQ ID NO: 225; SEQ ID NO: 226; SEQ ID NO: 227; SEQ ID NO: 228; SEQ ID NO: 229; SEQ ID NO: 230; SEQ ID NO: 231; SEQ ID NO: 232; SEQ ID NO: 233; SEQ ID NO: 234; SEQ ID NO: 235; SEQ ID NO: 236; SEQ ID NO: 237; SEQ ID NO: 238; SEQ ID NO: 239; SEQ ID NO: 240; SEQ ID NO: 241; SEQ ID NO: 242; SEQ ID NO: 243; SEQ ID NO: 244; SEQ ID NO: 245; SEQ ID NO: 246; SEQ ID NO: 247; SEQ ID NO: 248; SEQ ID NO: 249; SEQ ID NO: 250; SEQ ID NO: 251; SEQ ID NO: 252; SEQ ID NO: 253; SEQ ID NO: 254; SEQ ID NO: 255; SEQ ID NO: 256; SEQ ID NO: 257; SEQ ID NO: 258; SEQ ID NO: 259; SEQ ID NO: 260; SEQ ID NO: 261; SEQ ID NO: 262; SEQ ID NO: 263; SEQ ID NO: 264; SEQ ID NO: 265; SEQ ID NO: 266; SEQ ID NO: 267; SEQ ID NO: 268; SEQ ID NO: 269; SEQ ID NO: 270; SEQ ID NO: 271; SEQ ID NO: 272; SEQ ID NO: 273; SEQ ID NO: 274; SEQ ID NO: 275; SEQ ID NO: 276; SEQ ID NO: 277; SEQ ID NO: 278; SEQ ID NO: 279; SEQ ID NO: 280; SEQ ID NO: 281; SEQ ID NO: 282; SEQ ID NO: 283; SEQ ID NO: 284; SEQ ID NO: 285; SEQ ID NO: 286; SEQ ID NO: 287; SEQ ID NO: 288; SEQ ID NO: 289; SEQ ID NO: 290; SEQ ID NO: 291; SEQ ID NO: 292; SEQ ID NO: 293; SEQ ID NO: 294; SEQ ID NO: 295; SEQ ID NO: 296; SEQ ID NO: 297; SEQ ID NO: 298; SEQ ID NO: 299; SEQ ID NO: 300; SEQ ID NO: 301; SEQ ID NO: 302; SEQ ID NO: 303; SEQ ID NO: 304; SEQ ID NO: 305; SEQ ID NO: 306; SEQ ID NO: 307; SEQ ID NO: 308; SEQ ID NO: 309; SEQ ID NO: 310; SEQ ID NO: 311; SEQ ID NO: 312; SEQ ID NO: 313; SEQ ID NO:314; SEQ ID NO:315; SEQ ID NO:316; SEQ ID NO: 317; SEQ ID NO: 318; SEQ ID NO: 319; SEQ ID NO: 320; SEQ ID NO: 321; SEQ ID NO: 322; SEQ ID NO: 323; SEQ ID NO: 324; SEQ ID NO: 325; SEQ ID NO: 326; SEQ ID NO: 327; SEQ ID NO: 328; SEQ ID NO: 329; SEQ ID NO: 330; SEQ ID NO: 331; SEQ ID NO: 332; SEQ ID NO: 333; SEQ ID NO: 342; SEQ ID NO: 343; SEQ ID NO: 344; SEQ ID NO: 345; SEQ ID NO: 346; SEQ ID NO: 347; SEQ ID NO: 348; SEQ ID NO: 349; SEQ ID NO: 350; SEQ ID NO: 351; SEQ ID NO: 352; SEQ ID NO: 353; SEQ ID NO: 354; SEQ ID NO: 355; SEQ ID NO: 356; SEQ ID NO: 357; SEQ ID NO: 358; SEQ ID NO: 359; SEQ ID NO: 360; SEQ ID NO: 361; SEQ ID NO: 362; SEQ ID NO: 369; SEQ ID NO: 370; SEQ ID NO: 371; SEQ ID NO: 372; SEQ ID NO: 373; SEQ ID NO: 374; SEQ ID NO: 375; SEQ ID NO: 376; SEQ ID NO: 377; SEQ ID NO: 378; SEQ ID NO: 379; SEQ ID NO: 380; SEQ ID NO: 381; SEQ ID NO: 382; SEQ ID NO: 383; SEQ ID NO: 384; SEQ ID NO: 385; SEQ ID NO: 386; SEQ ID NO: 387; SEQ ID NO: 388; SEQ ID NO: 389; SEQ ID NO: 390; SEQ ID NO: 391; SEQ ID NO: 392; SEQ ID NO: 393; SEQ ID NO: 394; SEQ ID NO: 395; SEQ ID NO: 396; SEQ ID NO: 397; SEQ ID NO: 9528; SEQ ID NO: 9529; SEQ ID NO: 9530; SEQ ID NO: 9531; SEQ ID NO: 9532; SEQ ID NO: 9533; SEQ ID NO: 9534; SEQ ID NO: 9535, and SEQ ID NO: 9636 or a complement thereof.  
     [0059] In another embodiment, the  H. pylori  outer membrane polypeptide or a fragment thereof is an  H. pylori  polypeptide having a terminal phenylalanine residue and a C-terminal tyrosine cluster or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 326; SEQ ID NO: 327; SEQ ID NO: 328; SEQ ID NO: 329; SEQ ID NO: 330; SEQ ID NO: 331; SEQ ID NO: 332; SEQ ID NO: 333; SEQ ID NO: 342; SEQ ID NO: 343; SEQ ID NO: 344; SEQ ID NO: 345; SEQ ID NO: 346; SEQ ID NO: 347; SEQ ID NO: 348; SEQ ID NO: 349; SEQ ID NO: 350; SEQ ID NO: 351; SEQ ID NO: 352; SEQ ID NO: 353; SEQ ID NO: 354; SEQ ID NO: 355; SEQ ID NO: 356; SEQ ID NO: 357; SEQ ID NO: 358; SEQ ID NO: 359; SEQ ID NO: 360; SEQ ID NO: 361; SEQ ID NO: 362; SEQ ID NO: 369; SEQ ID NO: 370; SEQ ID NO: 371; SEQ ID NO: 372; SEQ ID NO: 373; SEQ ID NO: 374; SEQ ID NO: 375; SEQ ID NO: 376; SEQ ID NO: 377; SEQ ID NO: 378; SEQ ID NO: 379; SEQ ID NO: 380; SEQ ID NO: 381; SEQ ID NO: 382; SEQ ID NO: 383; SEQ ID NO: 384; SEQ ID NO: 385; SEQ ID NO: 386; SEQ ID NO: 387; SEQ ID NO: 388; SEQ ID NO: 389; SEQ ID NO: 390; SEQ ID NO: 391; SEQ ID NO: 392; SEQ ID NO: 393; SEQ ID NO: 394; SEQ ID NO: 395; SEQ ID NO: 396; SEQ ID NO: 397 and SEQ ID NO: 9536, or a complement thereof.  
     [0060] In another embodiment, the  H. pylori  outer membrane polypeptide or a fragment thereof is an  H. pylori  polypeptide having a terminal tyrosine cluster motif or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 326; SEQ ID NO: 327; SEQ ID NO: 328; SEQ ID NO: 329; SEQ ID NO: 330; SEQ ID NO: 331; SEQ ID NO: 332; SEQ ID NO: 333; SEQ ID NO: 334; SEQ ID NO: 335; SEQ ID NO: 336; SEQ ID NO: 337; SEQ ID NO: 338; SEQ ID NO: 339; SEQ ID NO: 340; SEQ ID NO: 341; SEQ ID NO: 342; SEQ ID NO: 343; SEQ ID NO: 344; SEQ ID NO: 345; SEQ ID NO: 346; SEQ ID NO: 347; SEQ ID NO: 348; SEQ ID NO: 349; SEQ ID NO: 350; SEQ ID NO: 351; SEQ ID NO: 352; SEQ ID NO: 353; SEQ ID NO: 354; SEQ ID NO: 355; SEQ ID NO: 356; SEQ ID NO: 357; SEQ ID NO: 358; SEQ ID NO: 359; SEQ ID NO: 360; SEQ ID NO: 361; SEQ ID NO: 362; SEQ ID NO: 363; SEQ ID NO: 364; SEQ ID NO: 365; SEQ ID NO: 366; SEQ ID NO: 367; SEQ ID NO: 368; SEQ ID NO: 369; SEQ ID NO: 370; SEQ ID NO: 371; SEQ ID NO: 372; SEQ ID NO: 373; SEQ ID NO: 374; SEQ ID NO: 375; SEQ ID NO: 376; SEQ ID NO: 377; SEQ ID NO: 378; SEQ ID NO: 379; SEQ ID NO: 380; SEQ ID NO: 381; SEQ ID NO: 382; SEQ ID NO: 383; SEQ ID NO: 384; SEQ ID NO: 385; SEQ ID NO: 386; SEQ ID NO: 387; SEQ ID NO: 388; SEQ ID NO: 389; SEQ ID NO: 390; SEQ ID NO: 391; SEQ ID NO: 392; SEQ ID NO: 393; SEQ ID NO: 394; SEQ ID NO: 395; SEQ ID NO: 396; SEQ ID NO: 397; SEQ ID NO: 398; SEQ ID NO: 399; SEQ ID NO: 400; SEQ ID NO: 401; SEQ ID NO: 402; SEQ ID NO: 403; SEQ ID NO: 404; SEQ ID NO: 405; SEQ ID NO: 406; SEQ ID NO: 407; SEQ ID NO: 408; SEQ ID NO: 409; SEQ ID NO:410; SEQ ID NO:411; SEQ ID NO:412; SEQ ID NO: 413; SEQ ID NO: 414; SEQ ID NO: 415; SEQ ID NO: 416; SEQ ID NO: 398; SEQ ID NO: 399; SEQ ID NO: 400; SEQ ID NO: 401; SEQ ID NO: 402; SEQ ID NO: 403; SEQ ID NO: 404; SEQ ID NO: 405; SEQ ID NO: 406; SEQ ID NO: 407; SEQ ID NO: 408; SEQ ID NO: 409; SEQ ID NO: 410; SEQ ID NO: 411; SEQ ID NO: 412; SEQ ID NO: 413; SEQ ID NO: 414; SEQ ID NO: 415; SEQ ID NO: 416; SEQ ID NO: 424; SEQ ID NO: 425 and SEQ ID NO: 9536, or a complement thereof.  
     [0061] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 511; SEQ ID NO: 512; SEQ ID NO: 513; SEQ ID NO: 514; SEQ ID NO: 515; SEQ ID NO: 516; SEQ ID NO: 517; SEQ ID NO: 518; SEQ ID NO: 519; SEQ ID NO: 520; SEQ ID NO: 521; SEQ ID NO: 522; SEQ ID NO: 523; SEQ ID NO: 524; SEQ ID NO: 525; SEQ ID NO: 526; SEQ ID NO: 527; SEQ ID NO: 528; SEQ ID NO: 529; SEQ ID NO: 530; SEQ ID NO: 531; SEQ ID NO: 532; SEQ ID NO: 533; SEQ ID NO: 534; SEQ ID NO: 535; SEQ ID NO: 536; SEQ ID NO: 537; SEQ ID NO: 538; SEQ ID NO: 539; SEQ ID NO: 540; SEQ ID NO: 541; SEQ ID NO: 542; SEQ ID NO: 543; SEQ ID NO: 544; SEQ ID NO: 545; SEQ ID NO: 546; SEQ ID NO: 547; SEQ ID NO: 548; SEQ ID NO: 549; SEQ ID NO: 550; SEQ ID NO: 551; SEQ ID NO: 552; SEQ ID NO: 553; SEQ ID NO: 554; SEQ ID NO: 555; SEQ ID NO: 556; SEQ ID NO: 557; SEQ ID NO: 558; SEQ ID NO: 559; SEQ ID NO: 560; SEQ ID NO: 561; SEQ ID NO: 562; SEQ ID NO: 563; SEQ ID NO: 564; SEQ ID NO: 565; SEQ ID NO: 566; SEQ ID NO: 567; SEQ ID NO: 568; SEQ ID NO: 569; SEQ ID NO: 570; SEQ ID NO: 571; SEQ ID NO: 572; SEQ ID NO: 573; SEQ ID NO: 574; SEQ ID NO: 575; SEQ ID NO: 576; SEQ ID NO: 577; SEQ ID NO: 578; SEQ ID NO: 579; SEQ ID NO: 580; SEQ ID NO: 581; SEQ ID NO: 582; SEQ ID NO: 583; SEQ ID NO: 584; SEQ ID NO: 585; SEQ ID NO: 586; SEQ ID NO: 587; SEQ ID NO: 588; SEQ ID NO: 589; SEQ ID NO: 590; SEQ ID NO: 591; SEQ ID NO: 592; SEQ ID NO: 593; SEQ ID NO: 594; SEQ ID NO: 595; SEQ ID NO: 596; SEQ ID NO: 597; SEQ ID NO: 598; SEQ ID NO: 599; SEQ ID NO: 600; SEQ ID NO: 601; SEQ ID NO: 602; SEQ ID NO: 603; SEQ ID NO: 604; SEQ ID NO: 605; SEQ ID NO: 606; SEQ ID NO: 607; SEQ ID NO: 608; SEQ ID NO: 609; SEQ ID NO: 610; SEQ ID NO: 611; SEQ ID NO: 612; SEQ ID NO: 613; SEQ ID NO: 614; SEQ ID NO: 615; SEQ ID NO: 616; SEQ ID NO: 617; SEQ ID NO: 618; SEQ ID NO: 619; SEQ ID NO: 620; SEQ ID NO: 621; SEQ ID NO: 622; SEQ ID NO: 623; SEQ ID NO: 624; SEQ ID NO: 625; SEQ ID NO: 626; SEQ ID NO: 627; SEQ ID NO: 628; SEQ ID NO: 629; SEQ ID NO: 630; SEQ ID NO: 631; SEQ ID NO: 632; SEQ ID NO: 633; SEQ ID NO: 634; SEQ ID NO: 635; SEQ ID NO: 636; SEQ ID NO: 637; SEQ ID NO: 638; SEQ ID NO: 639; SEQ ID NO: 640; SEQ ID NO: 641; SEQ ID NO: 642; SEQ ID NO: 643; SEQ ID NO: 644; SEQ ID NO: 645; SEQ ID NO: 646; SEQ ID NO: 647; SEQ ID NO: 648; SEQ ID NO: 649; SEQ ID NO: 650; SEQ ID NO: 651; SEQ ID NO: 652; SEQ ID NO: 653; SEQ ID NO: 654; SEQ ID NO: 655; SEQ ID NO: 656; SEQ ID NO: 657; SEQ ID NO: 658; SEQ ID NO: 659; SEQ ID NO: 660; SEQ ID NO: 661; SEQ ID NO: 662; SEQ ID NO: 663; SEQ ID NO: 664; SEQ ID NO: 665; SEQ ID NO: 666; SEQ ID NO: 667; SEQ ID NO: 668; SEQ ID NO: 669; SEQ ID NO: 670; SEQ ID NO: 671; SEQ ID NO: 672; SEQ ID NO: 673; SEQ ID NO: 674; SEQ ID NO: 675; SEQ ID NO: 676; SEQ ID NO: 677; SEQ ID NO: 678; SEQ ID NO: 679; SEQ ID NO: 680; SEQ ID NO: 681; SEQ ID NO: 682; SEQ ID NO: 683; SEQ ID NO: 684; SEQ ID NO: 685; SEQ ID NO: 686; SEQ ID NO: 687; SEQ ID NO: 688; SEQ ID NO: 689; SEQ ID NO: 690; SEQ ID NO: 691; SEQ ID NO: 692; SEQ ID NO: 693; SEQ ID NO: 694; SEQ ID NO: 695; SEQ ID NO: 696; SEQ ID NO: 697; SEQ ID NO: 698; SEQ ID NO: 699; SEQ ID NO: 700; SEQ ID NO: 701; SEQ ID NO: 702; SEQ ID NO: 703; SEQ ID NO: 704; SEQ ID NO: 705; SEQ ID NO: 706; SEQ ID NO: 707; SEQ ID NO: 708; SEQ ID NO: 709; SEQ ID NO: 710; SEQ ID NO: 711; SEQ ID NO: 712; SEQ ID NO: 713; SEQ ID NO: 714; SEQ ID NO: 715; SEQ ID NO: 716; SEQ ID NO: 717; SEQ ID NO: 718; SEQ ID NO: 719; SEQ ID NO: 720; SEQ ID NO: 721; SEQ ID NO: 722; SEQ ID NO: 723; SEQ ID NO: 724; SEQ ID NO: 725; SEQ ID NO: 726; SEQ ID NO: 727; SEQ ID NO: 728; SEQ ID NO: 729; SEQ ID NO: 730; SEQ ID NO: 731; SEQ ID NO: 732; SEQ ID NO: 733; SEQ ID NO: 734; SEQ ID NO: 735; SEQ ID NO: 736; SEQ ID NO: 737; SEQ ID NO: 738; SEQ ID NO: 739; SEQ ID NO: 740; SEQ ID NO: 741; SEQ ID NO: 742; SEQ ID NO: 743; SEQ ID NO: 744; SEQ ID NO: 745; SEQ ID NO: 746; SEQ ID NO: 747; SEQ ID NO: 748; SEQ ID NO: 749; SEQ ID NO: 750; SEQ ID NO: 751; SEQ ID NO: 752; SEQ ID NO: 753; SEQ ID NO: 754; SEQ ID NO: 755; SEQ ID NO: 756; SEQ ID NO: 757; SEQ ID NO: 758; SEQ ID NO: 759; SEQ ID NO: 760; SEQ ID NO: 761; SEQ ID NO: 762; SEQ ID NO: 763; SEQ ID NO: 764; SEQ ID NO: 765; SEQ ID NO: 766; SEQ ID NO: 767; SEQ ID NO: 768; SEQ ID NO: 769; SEQ ID NO: 770; SEQ ID NO: 771; SEQ ID NO: 772; SEQ ID NO: 773; SEQ ID NO: 774; SEQ ID NO: 775; SEQ ID NO: 776; SEQ ID NO: 777; SEQ ID NO: 778; SEQ ID NO: 779; SEQ ID NO: 780; SEQ ID NO: 781; SEQ ID NO: 782; SEQ ID NO: 783; SEQ ID NO: 784; SEQ ID NO: 785; SEQ ID NO: 786; SEQ ID NO: 787; SEQ ID NO: 788; SEQ ID NO: 789; SEQ ID NO: 790; SEQ ID NO: 791; SEQ ID NO: 792; SEQ ID NO: 793; SEQ ID NO: 794; SEQ ID NO: 795; SEQ ID NO: 796; SEQ ID NO: 797; SEQ ID NO: 798; SEQ ID NO: 799; SEQ ID NO: 800; SEQ ID NO: 801; SEQ ID NO: 802; SEQ ID NO: 803; SEQ ID NO: 804; SEQ ID NO: 805; SEQ ID NO: 806; SEQ ID NO: 807; SEQ ID NO: 808; SEQ ID NO: 809; SEQ ID NO: 810; SEQ ID NO: 811; SEQ ID NO: 812; SEQ ID NO: 813; SEQ ID NO: 814; SEQ ID NO: 815; SEQ ID NO: 816; SEQ ID NO: 817; SEQ ID NO: 818; SEQ ID NO: 819; SEQ ID NO: 820; SEQ ID NO: 821; SEQ ID NO: 822; SEQ ID NO: 823; SEQ ID NO: 824; SEQ ID NO: 825; SEQ ID NO: 826; SEQ ID NO: 827; SEQ ID NO: 828; SEQ ID NO: 829; SEQ ID NO: 830; SEQ ID NO: 831; SEQ ID NO: 832; SEQ ID NO: 833; SEQ ID NO: 834; SEQ ID NO: 835; SEQ ID NO: 836; SEQ ID NO: 837; SEQ ID NO: 838; SEQ ID NO: 839; SEQ ID NO: 840; SEQ ID NO: 841; SEQ ID NO: 842; SEQ ID NO: 843; SEQ ID NO: 844; SEQ ID NO: 845; SEQ ID NO: 846; SEQ ID NO: 847; SEQ ID NO: 848; SEQ ID NO: 849; SEQ ID NO: 850; SEQ ID NO: 851; SEQ ID NO: 852; SEQ ID NO: 853; SEQ ID NO: 854; SEQ ID NO: 855; SEQ ID NO: 856; SEQ ID NO: 857; SEQ ID NO: 858; SEQ ID NO: 859; SEQ ID NO: 860; SEQ ID NO: 861; SEQ ID NO: 862; SEQ ID NO: 863; SEQ ID NO: 864; SEQ ID NO: 865; SEQ ID NO: 866; SEQ ID NO: 867; SEQ ID NO: 868; SEQ ID NO: 869; SEQ ID NO: 870; SEQ ID NO: 871; SEQ ID NO: 872; SEQ ID NO: 873; SEQ ID NO: 874; SEQ ID NO: 875; SEQ ID NO: 876; SEQ ID NO: 877; SEQ ID NO: 878; SEQ ID NO: 879; SEQ ID NO: 880; SEQ ID NO: 881; SEQ ID NO: 882; SEQ ID NO: 883; SEQ ID NO: 884; SEQ ID NO: 885; SEQ ID NO: 886; SEQ ID NO: 887; SEQ ID NO: 888; SEQ ID NO: 889; SEQ ID NO: 890; SEQ ID NO: 891; SEQ ID NO: 892; SEQ ID NO:893; SEQ ID NO: 894; SEQ ID NO: 895; SEQ ID NO: 896; SEQ ID NO: 897; SEQ ID NO: 898; SEQ ID NO: 899; SEQ ID NO: 900; SEQ ID NO: 901; SEQ ID NO: 902; SEQ ID NO: 903; SEQ ID NO: 904; SEQ ID NO: 905; SEQ ID NO: 906; SEQ ID NO: 907; SEQ ID NO: 908; SEQ ID NO: 909; SEQ ID NO: 910; SEQ ID NO: 911; SEQ ID NO: 912; SEQ ID NO: 913; SEQ ID NO: 914; SEQ ID NO: 915; SEQ ID NO: 916; SEQ ID NO: 917; SEQ ID NO: 918; SEQ ID NO: 919; SEQ ID NO: 920; SEQ ID NO: 921; SEQ ID NO: 922; SEQ ID NO: 923; SEQ ID NO: 924; SEQ ID NO: 925; SEQ ID NO: 926; SEQ ID NO: 927; SEQ ID NO: 928; SEQ ID NO: 929; SEQ ID NO: 930; SEQ ID NO: 931; SEQ ID NO: 932; SEQ ID NO: 933; SEQ ID NO: 934; SEQ ID NO: 935; SEQ ID NO: 936; SEQ ID NO: 937; SEQ ID NO: 938; SEQ ID NO: 939; SEQ ID NO: 940; SEQ ID NO: 941; SEQ ID NO: 942; SEQ ID NO: 943; SEQ ID NO: 944; SEQ ID NO: 945; SEQ ID NO: 946; SEQ ID NO: 947; SEQ ID NO: 948; SEQ ID NO: 949; SEQ ID NO: 950; SEQ ID NO: 951; SEQ ID NO: 952; SEQ ID NO: 953; SEQ ID NO: 954; SEQ ID NO: 955; SEQ ID NO: 956; SEQ ID NO: 957; SEQ ID NO: 958; SEQ ID NO: 959; SEQ ID NO: 960; SEQ ID NO: 961; SEQ ID NO: 962; SEQ ID NO: 963; SEQ ID NO: 964; SEQ ID NO: 965; SEQ ID NO: 966; SEQ ID NO: 967; SEQ ID NO: 968; SEQ ID NO: 969; SEQ ID NO: 970; SEQ ID NO: 971; SEQ ID NO: 972; SEQ ID NO: 973; SEQ ID NO: 974; SEQ ID NO: 975; SEQ ID NO: 976; SEQ ID NO: 977; SEQ ID NO: 978; SEQ ID NO: 979; SEQ ID NO: 980; SEQ ID NO: 981; SEQ ID NO: 982; SEQ ID NO: 983; SEQ ID NO: 984; SEQ ID NO: 985; SEQ ID NO: 986; SEQ ID NO: 987; SEQ ID NO: 988; SEQ ID NO: 989; SEQ ID NO: 990; SEQ ID NO: 991; SEQ ID NO: 992; SEQ ID NO: 993; SEQ ID NO: 994; SEQ ID NO: 995; SEQ ID NO: 996; SEQ ID NO: 997; SEQ ID NO: 998; SEQ ID NO: 999; SEQ ID NO: 1000; SEQ ID NO: 1001; SEQ ID NO: 1002; SEQ ID NO: 1003; SEQ ID NO: 1004; SEQ ID NO: 1005; SEQ ID NO: 1006; SEQ ID NO: 1007; SEQ ID NO: 1008; SEQ ID NO: 1009; SEQ ID NO: 1010; SEQ ID NO: 1011; SEQ ID NO: 1012; SEQ ID NO: 1013; SEQ ID NO: 1014; SEQ ID NO: 1015; SEQ ID NO: 1016; SEQ ID NO: 1017; SEQ ID NO: 1018; SEQ ID NO: 1019; SEQ ID NO: 1020; SEQ ID NO: 1021; SEQ ID NO: 1022; SEQ ID NO: 1023; SEQ ID NO: 1024; SEQ ID NO: 1025; SEQ ID NO: 1026; SEQ ID NO: 1027; SEQ ID NO: 1028; SEQ ID NO: 1029; SEQ ID NO: 1030; SEQ ID NO: 1031; SEQ ID NO: 1032; SEQ ID NO: 1033; SEQ ID NO: 1034; SEQ ID NO: 1035; SEQ ID NO: 1036; SEQ ID NO: 1037; SEQ ID NO: 1038; SEQ ID NO: 1039; SEQ ID NO: 1040; SEQ ID NO: 1041; SEQ ID NO: 1042; SEQ ID NO: 1043; SEQ ID NO: 1044; SEQ ID NO: 1045; SEQ ID NO: 1046; SEQ ID NO: 1047; SEQ ID NO: 1048; SEQ ID NO: 1049; SEQ ID NO: 1050; SEQ ID NO: 1051; SEQ ID NO: 1052; SEQ ID NO: 1053; SEQ ID NO: 1054; SEQ ID NO: 1055; SEQ ID ND 1056; SEQ ID NO: 1057; SEQ ID NO: 1058; SEQ ID NO: 1059; SEQ ID NO: 1060; SEQ ID NO: 1061; SEQ ID NO: 1062; SEQ ID NO: 1063; SEQ ID NO: 1064; SEQ ID NO: 1065; SEQ ID NO: 1066; SEQ ID NO: 1067; SEQ ID NO: 1068; SEQ ID NO: 1069; SEQ ID NO: 1070; SEQ ID NO: 1071; SEQ ID NO: 1072; SEQ ID NO: 1073; SEQ ID NO: 1074; SEQ ID NO: 1075; SEQ ID NO: 1076; SEQ ID NO: 1077; SEQ ID NO: 1078; SEQ ID NO: 1079; SEQ ID NO: 1080; SEQ ID NO: 1081; SEQ ID NO: 1082; SEQ ID NO: 1083; SEQ ID NO: 1084; SEQ ID NO: 1085; SEQ ID NO: 1086; SEQ ID NO: 1087; SEQ ID NO: 1088; SEQ ID NO: 1089; SEQ ID NO: 1090; SEQ ID NO: 1091; SEQ ID NO: 1092; SEQ ID NO: 1093; SEQ ID NO: 1094; SEQ ID NO: 1095; SEQ ID NO: 1096; SEQ ID NO: 1097; SEQ ID NO: 1098; SEQ ID NO: 1099; SEQ ID NO: 1100; SEQ ID NO: 1101; SEQ ID NO: 1102; SEQ ID NO: 1103; SEQ ID NO: 1104; SEQ ID NO: 1105; SEQ ID NO: 1106; SEQ ID NO: 1107; SEQ ID NO: 1108; SEQ ID NO: 1109; SEQ ID NO: 1110; SEQ ID NO: 1111; SEQ ID NO: 1112; SEQ ID NO: 1113; SEQ ID NO: 1114; SEQ ID NO: 1115; SEQ ID NO: 1116; SEQ ID NO: 1117; SEQ ID NO: 1118; SEQ ID NO: 1119; SEQ ID NO: 1120; SEQ ID NO: 1121; SEQ ID NO: 1122; SEQ ID NO: 1123; SEQ ID NO: 1124; SEQ ID NO: 1125; SEQ ID NO: 1126; SEQ ID NO: 1127; SEQ ID NO: 1128; SEQ ID NO: 1129; SEQ ID NO: 1130; SEQ ID NO: 1131; SEQ ID NO: 1132; SEQ ID NO: 1133; SEQ ID NO: 1134; SEQ ID NO: 1135; SEQ ID NO: 1136; SEQ ID NO: 1137; SEQ ID NO: 1138; SEQ ID NO: 1139; SEQ ID NO: 1140; SEQ ID NO: 1141; SEQ ID NO: 1142; SEQ ID NO: 1143; SEQ ID NO: 1144; SEQ ID NO: 1145; SEQ ID NO: 1146; SEQ ID NO: 1147; SEQ ID NO: 1148; SEQ ID NO: 1149; SEQ ID NO: 1150; SEQ ID NO: 1151; SEQ ID NO: 1152; SEQ ID NO: 1153; SEQ ID NO: 1154; SEQ ID NO: 1155; SEQ ID NO: 1156; SEQ ID NO: 1157; SEQ ID NO: 1158; SEQ ID NO: 1159; SEQ ID NO: 1160; SEQ ID NO: 1161; SEQ ID NO: 1162; SEQ ID NO: 1163; SEQ ID NO: 1164; SEQ ID NO: 1165; SEQ ID NO: 1166; SEQ ID NO: 1167; SEQ ID NO: 1168; SEQ ID NO: 1169; SEQ ID NO: 1170; SEQ ID NO: 1171; SEQ ID NO: 1172; SEQ ID NO: 1173; SEQ ID NO: 1174; SEQ ID NO: 1175; SEQ ID NO: 1176; SEQ ID NO: 1177; SEQ ID NO: 1178; SEQ ID NO: 1179; SEQ ID NO: 1180; SEQ ID NO: 1181; SEQ ID NO: 1182; SEQ ID NO: 1183; SEQ ID NO: 1184; SEQ ID NO: 1185; SEQ ID NO: 1186; SEQ ID NO: 1187; SEQ ID NO: 1188; SEQ ID NO: 118Q9 SEQ ID NO: 1190; SEQ ID NO: 1191; SEQ ID NO: 1192; SEQ ID NO: 1193; SEQ ID NO: 1194; SEQ ID NO: 1195; SEQ ID NO: 1196; SEQ ID NO: 1197; SEQ ID NO: 1198; SEQ ID NO: 1199; SEQ ID NO: 1200; SEQ ID NO: 1201; SEQ ID NO: 1202; SEQ ID NO: 1203; SEQ ID NO: 1204; SEQ ID NO: 1205; SEQ ID NO: 1206; SEQ ID NO: 1207; SEQ ID NO: 1208; SEQ ID NO: 1209; SEQ ID NO: 1210; SEQ ID NO:  121 I; SEQ ID NO: 1212; SEQ ID NO: 1213; SEQ ID NO: 1214; SEQ ID NO: 1215; SEQ ID NO: 1216; SEQ ID NO: 1217; SEQ ID NO: 1218; SEQ ID NO: 1219; SEQ ID NO: 1220; SEQ ID NO: 1221; SEQ ID NO: 1222; SEQ ID NO: 1223; SEQ ID NO: 1224; SEQ ID NO: 1225; SEQ ID NO: 1226; SEQ ID NO: 1227; SEQ ID NO: 1228; SEQ ID NO: 1229; SEQ ID NO: 1230; SEQ ID NO: 1231; SEQ ID NO: 1232; SEQ ID NO: 1233; SEQ ID NO: 1234; SEQ ID NO: 1235; SEQ ID NO: 1236; SEQ ID NO: 1237; SEQ ID NO: 1238; SEQ ID NO: 1239; SEQ ID NO: 1240; SEQ ID NO: 1241; SEQ ID NO: 1242; SEQ ID NO: 1243; SEQ ID NO: 1244; SEQ ID NO: 1245; SEQ ID NO: 1246; SEQ ID NO: 1247; SEQ ID NO: 1248; SEQ ID NO: 1249; SEQ ID NO: 1250; SEQ ID NO: 1251; SEQ ID NO: 1252; SEQ ID NO: 1253; SEQ ID NO: 1254; SEQ ID NO: 1255; SEQ ID NO: 1256; SEQ ID NO: 1257; SEQ ID NO: 1258; SEQ ID NO: 1259; SEQ ID NO: 1260; SEQ ID NO: 1261; SEQ ID NO: 1262; SEQ ID NO: 1263; SEQ ID NO: 1264; SEQ ID NO: 1265; SEQ ID NO: 1266; SEQ ID NO: 1267; SEQ ID NO: 1268; SEQ ID NO: 1269; SEQ ID NO: 1270; SEQ ID NO: 1271; SEQ ID NO: 1272; SEQ ID NO: 1273; SEQ ID NO: 1274; SEQ ID NO: 1275; SEQ ID NO: 1276; SEQ ID NO: 1277; SEQ ID NO: 1278; SEQ ID NO: 1279; SEQ ID NO: 1280; SEQ ID NO: 1281; SEQ ID NO: 1282; SEQ ID NO: 1283; SEQ ID NO: 1284; SEQ ID NO: 1285; SEQ ID NO: 1286; SEQ ID NO: 1287; SEQ ID NO: 1288; SEQ ID NO: 1289; SEQ ID NO: 1290; SEQ ID NO: 1291; SEQ ID NO: 1292; SEQ ID NO: 1293; SEQ ID NO: 1294; SEQ ID NO: 1295; SEQ ID NO: 1296; SEQ ID NO: 1297; SEQ ID NO: 1298; SEQ ID NO: 1299; SEQ ID NO: 1300; SEQ ID NO: 1301; SEQ ID NO: 1302; SEQ ID NO: 1303; SEQ ID NO: 1304; SEQ ID NO: 1305; SEQ ID NO: 1306; SEQ ID NO: 1307; SEQ ID NO: 1308; SEQ ID NO: 1309; SEQ ID NO: 1310; SEQ ID NO:  131 I; SEQ ID NO: 1312; SEQ ID NO: 1313; SEQ ID NO: 1314; SEQ ID NO: 1315; SEQ ID NO: 1316; SEQ ID NO: 1317; SEQ ID NO: 1318; SEQ ID NO: 1319; SEQ ID NO: 1320; SEQ ID NO: 1321; SEQ ID NO: 1322; SEQ ID NO: 1323; SEQ ID NO: 1324; SEQ ID NO: 1325; SEQ ID NO: 1326; SEQ ID NO: 1327; SEQ ID NO: 1328; SEQ ID NO: 1329; SEQ ID NO: 1330; SEQ ID NO: 1331; SEQ ID NO: 1332; SEQ ID NO: 1333; SEQ ID NO: 1334; SEQ ID NO: 1335; SEQ ID NO: 1336; SEQ ID NO: 1337; SEQ ID NO: 1338; SEQ ID NO: 1339; SEQ ID NO: 1340; SEQ ID NO: 1341; SEQ ID NO: 1342; SEQ ID NO: 1343; SEQ ID NO: 1344; SEQ ID NO: 1345; SEQ ID NO: 1346; SEQ ID NO: 1347; SEQ ID NO: 1348; SEQ ID NO: 1349; SEQ ID NO: 1350; SEQ ID NO:  135 I; SEQ ID NO: 1352; SEQ ID NO: 1353; SEQ ID NO: 1354; SEQ ID NO: 1355; SEQ ID NO: 1356; SEQ ID NO: 1357; SEQ ID NO: 1358; SEQ ID NO: 1359; SEQ ID NO: 1360; SEQ ID NO: 1361; SEQ ID NO: 1362; SEQ ID NO: 1363; SEQ ID NO: 1364; SEQ ID NO: 1365; SEQ ID NO: 1366; SEQ ID NO: 1367; SEQ ID NO: 1368; SEQ ID NO: 1369; SEQ ID NO: 1370; SEQ ID NO: 1371; SEQ ID NO: 1372; SEQ ID NO: 1373; SEQ ID NO: 1374; SEQ ID NO: 1375; SEQ ID NO: 1376; SEQ ID NO: 1377; SEQ ID NO: 1378; SEQ ID NO: 1379; SEQ ID NO: 1380; SEQ ID NO: 1381; SEQ ID NO: 1382; SEQ ID NO: 1383; SEQ ID NO: 1384; SEQ ID NO: 1385; SEQ ID NO: 1386; SEQ ID NO: 1387; SEQ ID NO: 1388; SEQ ID NO: 1389; SEQ ID NO: 1390; SEQ ID NO: 1391; SEQ ID NO: 1392; SEQ ID NO: 1393; SEQ ID NO: 1394; SEQ ID NO: 1395; SEQ ID NO: 1396; SEQ ID NO: 1397; SEQ ID NO: 1398; SEQ ID NO: 1399; SEQ ID NO: 1400; SEQ ID NO: 1401; SEQ ID NO: 1402; SEQ ID NO: 1403; SEQ ID NO: 1404; SEQ ID NO: 1405; SEQ ID NO: 1406; SEQ ID NO: 1407; SEQ ID NO: 1408; SEQ ID NO: 1409; SEQ ID NO: 1410; SEQ ID NO: 1411; SEQ ID NO: 1412; SEQ ID NO: 1413; SEQ ID NO: 1414; SEQ ID NO: 1415; SEQ ID NO: 1416; SEQ ID NO: 1417; SEQ ID NO: 1418; SEQ ID NO: 1419; SEQ ID NO: 1420; SEQ ID NO: 1421; SEQ ID NO: 1422; SEQ ID NO: 1423; SEQ ID NO: 1424; SEQ ID NO: 1425; SEQ ID NO: 1426; SEQ ID NO: 1427; SEQ ID NO: 1428; SEQ ID NO: 1429; SEQ ID NO: 1430; SEQ ID NO: 1431; SEQ ID NO: 1432; SEQ ID NO: 1433; SEQ ID NO: 1434; SEQ ID NO: 1435; SEQ ID NO: 1436; SEQ ID NO: 1437; SEQ ID NO: 1438; SEQ ID NO: 1439; SEQ ID NO: 1440; SEQ ID NO: 1441; SEQ ID NO: 1442; SEQ ID NO: 1443; SEQ ID NO: 1444; SEQ ID NO: 1445; SEQ ID NO: 1446; SEQ ID NO: 1447; SEQ ID NO: 1448; SEQ ID NO: 1449; SEQ ID NO: 1450; SEQ ID NO: 1451; SEQ ID NO: 1452; SEQ ID NO: 1453; SEQ ID NO: 1454; SEQ ID NO: 1455; SEQ ID NO: 1456; SEQ ID NO: 1457; SEQ ID NO: 1458; SEQ ID NO: 1459; SEQ ID NO: 1460; SEQ ID NO: 1461; SEQ ID NO: 1462; SEQ ID NO: 1463; SEQ ID NO: 1464; SEQ ID NO: 1465; SEQ ID NO: 1466; SEQ ID NO: 1467; SEQ ID NO: 1468; SEQ ID NO: 1469; SEQ ID NO: 1470; SEQ ID NO: 1471; SEQ ID NO: 1472; SEQ ID NO: 1473; SEQ ID NO: 1474; SEQ ID NO: 1475; SEQ ID NO: 1476; SEQ ID NO: 1477; SEQ ID NO: 1478; SEQ ID NO: 1479; SEQ ID NO: 1480; SEQ ID NO: 1481; SEQ ID NO: 1482; SEQ ID NO: 1483; SEQ ID NO: 1484; SEQ ID NO: 1485; SEQ ID NO: 1486; SEQ ID NO: 1487; SEQ ID NO: 1488; SEQ ID NO: 1489; SEQ ID NO: 1490; SEQ ID NO: 1491; SEQ ID NO: 1492; SEQ ID NO: 1493; SEQ ID NO: 1494; SEQ ID NO: 1495; SEQ ID NO: 1496; SEQ ID NO: 1497; SEQ ID NO: 1498; SEQ ID NO: 1499; SEQ ID NO: 1500; SEQ ID NO: 1501; SEQ ID NO: 1502; SEQ ID NO: 1503; SEQ ID NO: 1504; SEQ ID NO: 1505; SEQ ID NO: 1506; SEQ ID NO: 1507; SEQ ID NO: 1508; SEQ ID NO: 1509; SEQ ID NO: 1510; SEQ ID NO: 1511; SEQ ID NO: 1512; SEQ ID NO: 1513; SEQ ID NO: 1514; and SEQ ID NO: 9537; SEQ ID NO: 9538; SEQ ID NO: 9539; SEQ ID NO: 9540; SEQ ID NO: 9541; SEQ ID NO: 9542; SEQ ID NO: 9543; SEQ ID NO: 9544; SEQ ID NO: 9545; SEQ ID NO: 9546; SEQ ID NO: 9547; SEQ ID NO: 9548; SEQ ID NO: 9549; SEQ ID NO: 9550; SEQ ID NO: 9551; SEQ ID NO: 9552; SEQ ID NO: 9553; SEQ ID NO: 9554; SEQ ID NO: 9555; SEQ ID NO: 9556; SEQ ID NO: 9557; SEQ ID NO: 9558; SEQ ID NO: 9559; SEQ ID NO: 9560; SEQ ID NO: 9561; SEQ ID NO: 9562; SEQ ID NO: 9563; SEQ ID NO: 9564; SEQ ID NO: 9565; SEQ ID NO: 9566; SEQ ID NO: 9567; SEQ ID NO: 9568; SEQ ID NO: 9569; SEQ ID NO: 9570; SEQ ID NO: 9571; SEQ ID NO: 9572; SEQ ID NO: 9573; SEQ ID NO: 9574; SEQ ID NO: 9575; SEQ ID NO: 9576; SEQ ID NO: 9577; SEQ ID NO: 9578; SEQ ID NO: 9579; SEQ ID NO: 9580; SEQ ID NO: 9581; SEQ ID NO: 9582; SEQ ID NO: 9583; SEQ ID NO: 9584; SEQ ID NO: 9585; SEQ ID NO: 9586; SEQ ID NO: 9587; SEQ ID NO: 9588; SEQ ID NO: 9589; SEQ ID NO: 9590; and SEQ ID NO: 9591, or a complement thereof.  
     [0062] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in transport encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 511; SEQ ID NO: 512; SEQ ID NO: 513; SEQ ID NO: 514; SEQ ID NO: 515; SEQ ID NO: 516; SEQ ID NO: 517; SEQ ID NO: 518; SEQ ID NO: 519; SEQ ID NO: 520; SEQ ID NO: 521; SEQ ID NO: 522; SEQ ID NO: 523; SEQ ID NO: 524; SEQ ID NO: 525; SEQ ID NO: 526; SEQ ID NO: 527; SEQ ID NO: 528; SEQ ID NO: 529; SEQ ID NO: 530; SEQ ID NO: 531; SEQ ID NO: 532; SEQ ID NO: 533; SEQ ID NO: 534; SEQ ID NO: 535; SEQ ID NO: 536; SEQ ID NO: 537; SEQ ID NO: 538; SEQ ID NO: 539; SEQ ID NO: 540; SEQ ID NO: 541; SEQ ID NO: 542; SEQ ID NO: 543; SEQ ID NO: 544; SEQ ID NO: 545; SEQ ID NO: 546; SEQ ID NO: 547; SEQ ID NO: 548; SEQ ID NO: 549; SEQ ID NO: 550, SEQ ID NO: 551; SEQ ID NO: 552; SEQ ID NO: 553; SEQ ID NO: 554; SEQ ID NO: 555; SEQ ID NO: 556; SEQ ID NO: 557; SEQ ID NO: 558; SEQ ID NO: 559; SEQ ID NO: 560; SEQ ID NO: 561; SEQ ID NO: 562; SEQ ID NO: 563; SEQ ID NO: 564; SEQ ID NO: 565; SEQ ID NO: 566; SEQ ID NO: 567; SEQ ID NO: 568; SEQ ID NO: 569; SEQ ID NO: 570; SEQ ID NO: 571; SEQ ID NO: 572; SEQ ID NO: 573; SEQ ID NO: 574; SEQ ID NO: 575; SEQ ID NO: 576; SEQ ID NO: 577; SEQ ID NO: 578; SEQ ID NO: 579; SEQ ID NO: 580; SEQ ID NO: 581; SEQ ID NO: 582; SEQ ID NO: 583; SEQ ID NO: 584; SEQ ID NO: 585; SEQ ID NO: 586; SEQ ID NO: 587; SEQ ID NO: 588; SEQ ID NO: 589; SEQ ID NO: 590; SEQ ID NO: 591; SEQ ID NO: 592; SEQ ID NO: 593; SEQ ID NO: 594; SEQ ID NO: 595; SEQ ID NO: 596; SEQ ID NO: 597; SEQ ID NO: 598; SEQ ID NO: 599; SEQ ID NO: 600; SEQ ID NO: 601; SEQ ID NO: 602; SEQ ID NO: 603; SEQ ID NO: 604; SEQ ID NO: 605; SEQ ID NO: 606; SEQ ID NO: 607; SEQ ID NO: 608; SEQ ID NO: 609; SEQ ID NO: 610; SEQ ID NO: 611; SEQ ID NO: 612; SEQ ID NO: 613; SEQ ID NO: 614; SEQ ID NO: 615; SEQ ID NO: 616; SEQ ID NO: 617; SEQ ID NO: 618; SEQ ID NO: 619; SEQ ID NO: 620; SEQ ID NO: 621; SEQ ID NO: 622; SEQ ID NO: 623; SEQ ID NO: 624; SEQ ID NO: 625; SEQ ID NO: 626; SEQ ID NO: 627; SEQ ID NO: 628; SEQ ID NO: 629; SEQ ID NO: 630; SEQ ID NO: 631; SEQ ID NO: 632; SEQ ID NO: 633; SEQ ID NO: 634; SEQ ID NO: 635; SEQ ID NO: 636; SEQ ID NO: 637; SEQ ID NO: 638; SEQ ID NO: 639; SEQ ID NO: 640; SEQ ID NO: 641; SEQ ID NO: 642; SEQ ID NO: 643; SEQ ID NO: 644; SEQ ID NO: 645; SEQ ID NO: 646; SEQ ID NO: 647; SEQ ID NO: 648; SEQ ID NO: 649; SEQ ID NO: 650; SEQ ID NO: 651; SEQ ID NO: 652; SEQ ID NO: 653; SEQ ID NO: 654; SEQ ID NO: 655; SEQ ID NO: 656; SEQ ID NO: 657; SEQ ID NO: 658; SEQ ID NO: 659; SEQ ID NO: 660; SEQ ID NO: 661; SEQ ID NO: 662; SEQ ID NO: 663; SEQ ID NO: 664; SEQ ID NO: 665; SEQ ID NO: 666; SEQ ID NO: 667; SEQ ID NO: 668; SEQ ID NO: 669; SEQ ID NO: 670; SEQ ID NO: 671; SEQ ID NO: 672; SEQ ID NO: 673; SEQ ID NO: 674; SEQ ID NO: 675; SEQ ID NO: 676; SEQ ID NO: 677; SEQ ID NO: 678; SEQ ID NO: 679; SEQ ID NO: 680; SEQ ID NO: 681; SEQ ID NO: 682; SEQ ID NO: 683; SEQ ID NO: 684; SEQ ID NO: 685; SEQ ID NO: 686; SEQ ID NO: 687; SEQ ID NO: 688; SEQ ID NO: 689; SEQ ID NO: 690; SEQ ID NO: 691; SEQ ID NO: 692; SEQ ID NO: 693; SEQ ID NO: 694; SEQ ID NO: 695; SEQ ID NO: 696; SEQ ID NO: 697; SEQ ID NO: 698; SEQ ID NO: 699; SEQ ID NO: 700; SEQ ID NO: 701; SEQ ID NO: 702; SEQ ID NO: 703; SEQ ID NO: 704; SEQ ID NO: 705; SEQ ID NO: 706; SEQ ID NO: 707; SEQ ID NO: 708; SEQ ID NO: 709; SEQ ID NO: 710; SEQ ID NO: 711; SEQ ID NO: 712; SEQ ID NO: 713; SEQ ID NO: 714; SEQ ID NO: 715; SEQ ID NO: 716; SEQ ID NO: 717; SEQ ID NO: 718; SEQ ID NO: 719; SEQ ID NO: 720; SEQ ID NO: 721; SEQ ID NO: 722; SEQ ID NO: 723; SEQ ID NO: 724; SEQ ID NO: 725; SEQ ID NO: 726; SEQ ID NO: 727; SEQ ID NO: 728; SEQ ID NO: 729; SEQ ID NO: 730; SEQ ID NO: 731; SEQ ID NO: 732; SEQ ID NO: 733; SEQ ID NO: 734; SEQ ID NO: 735; SEQ ID NO: 736; SEQ ID NO: 737; SEQ ID NO: 738; SEQ ID NO: 739; SEQ ID NO: 740; SEQ ID NO: 741; SEQ ID NO: 742; SEQ ID NO: 743; SEQ ID NO: 744; SEQ ID NO: 745; SEQ ID NO: 746; SEQ ID NO: 747; SEQ ID NO: 748; SEQ ID NO: 749; SEQ ID NO: 750; SEQ ID NO: 751; SEQ ID NO: 752; SEQ ID NO: 753; SEQ ID NO: 754; SEQ ID NO: 755; SEQ ID NO: 756; SEQ ID NO: 757; SEQ ID NO: 758; SEQ ID NO: 759; SEQ ID NO: 760; SEQ ID NO: 761; SEQ ID NO: 762; SEQ ID NO: 763; SEQ ID NO: 764; SEQ ID NO: 765; SEQ ID NO: 766; SEQ ID NO: 767; SEQ ID NO: 768; SEQ ID NO: 769; SEQ ID NO: 770; SEQ ID NO: 771; SEQ ID NO: 772; SEQ ID NO: 773; SEQ ID NO: 774; SEQ ID NO: 775; SEQ ID NO: 776; SEQ ID NO: 777; SEQ ID NO: 778; SEQ ID NO: 779; SEQ ID NO: 780; SEQ ID NO: 781; SEQ ID NO: 782; SEQ ID NO: 783; SEQ ID NO: 784; SEQ ID NO: 785; SEQ ID NO: 786; SEQ ID NO: 787; SEQ ID NO: 788; SEQ ID NO: 789; SEQ ID NO: 790; SEQ ID NO: 791; SEQ ID NO: 792; SEQ ID NO: 793; SEQ ID NO: 794; SEQ ID NO: 795; SEQ ID NO: 796; SEQ ID NO: 797; SEQ ID NO: 798; SEQ ID NO: 799; SEQ ID NO: 800; SEQ ID NO: 801; SEQ ID NO: 802; and SEQ ID NO: 9537; SEQ ID NO: 9538; SEQ ID NO: 9539; SEQ ID NO: 9540; SEQ ID NO: 9541; SEQ ID NO: 9542; SEQ ID NO: 9543; SEQ ID NO: 9544; SEQ ID NO: 9545; SEQ ID NO: 9546; SEQ ID NO: 9547; SEQ ID NO: 9548; SEQ ID NO: 9549; SEQ ID NO: 9550; SEQ ID NO: 9551; SEQ ID NO: 9552; SEQ ID NO: 9553; and SEQ ID NO: 9554, or a complement thereof.  
     [0063] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in amino acid metabolism and transport encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 683; SEQ ID NO: 684; SEQ ID NO: 685; SEQ ID NO: 686; SEQ ID NO: 687; SEQ ID NO: 688; SEQ ID NO: 689; SEQ ID NO: 690; SEQ ID NO: 691; SEQ ID NO: 692; SEQ ID NO: 693; SEQ ID NO: 694; SEQ ID NO: 695; SEQ ID NO: 696; SEQ ID NO: 697; SEQ ID NO: 698; SEQ ID NO: 699; SEQ ID NO: 700; SEQ ID NO: 701; SEQ ID NO: 702; SEQ ID NO: 703; SEQ ID NO: 704; SEQ ID NO: 705; SEQ ID NO: 706; SEQ ID NO: 707; SEQ ID NO: 708; SEQ ID NO: 709; SEQ ID NO: 710; SEQ ID NO: 711; SEQ ID NO: 712; SEQ ID NO: 713; SEQ ID NO: 714; SEQ ID NO: 715; SEQ ID NO: 716; SEQ ID NO: 717; SEQ ID NO: 718; SEQ ID NO: 719; SEQ ID NO: 720; and SEQ ID NO: 9547 and SEQ ID NO: 9548, or a complement thereof.  
     [0064] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in nucleotide, lipid, or cofactor metabolism and transport encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 721; SEQ ID NO: 722; SEQ ID NO: 723; SEQ ID NO: 724; SEQ ID NO: 725; SEQ ID NO: 726; SEQ ID NO: 727; SEQ ID NO: 728; SEQ ID NO: 729; SEQ ID NO: 730; SEQ ID NO: 731; SEQ ID NO: 732; SEQ ID NO: 733; SEQ ID NO: 734; SEQ ID NO: 735; SEQ ID NO: 736; SEQ ID NO: 737; SEQ ID NO: 738; SEQ ID NO: 739; SEQ ID NO: 740; SEQ ID NO: 741; SEQ ID NO: 742; SEQ ID NO: 743; SEQ ID NO: 744; SEQ ID NO: 745; SEQ ID NO: 746; SEQ ID NO: 747; SEQ ID NO: 748; SEQ ID NO: 749; SEQ ID NO: 750; SEQ ID NO: 751; SEQ ID NO: 752; SEQ ID NO: 753; SEQ ID NO: 754; SEQ ID NO: 755; SEQ ID NO: 756; SEQ ID NO: 757; SEQ ID NO: 758; SEQ ID NO: 759; SEQ ID NO: 760; SEQ ID NO: 761; SEQ ID NO: 762; SEQ ID NO: 763; SEQ ID NO: 764 and SEQ ID NO: 9549, or a complement thereof.  
     [0065] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in inorganic ion transport encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 765; SEQ ID NO: 766; SEQ ID NO: 767; SEQ ID NO: 768; SEQ ID NO: 769; SEQ ID NO: 770; SEQ ID NO: 771; SEQ ID NO: 772; SEQ ID NO: 773; SEQ ID NO: 774; SEQ ID NO: 775; SEQ ID NO: 776; SEQ ID NO: 777; SEQ ID NO: 778; SEQ ID NO: 779; SEQ ID NO: 780; SEQ ID NO: 781; SEQ ID NO: 782; SEQ ID NO: 783; SEQ ID NO: 784; SEQ ID NO: 785; SEQ ID NO: 786; SEQ ID NO: 787; SEQ ID NO: 788; SEQ ID NO: 789; SEQ ID NO: 790; SEQ ID NO: 791; SEQ ID NQ: 792; SEQ ID NO: 793; SEQ ID NO: 794; SEQ ID NO: 795; SEQ ID NO: 796; SEQ ID NO: 797; SEQ ID NO: 798; SEQ ID NO: 799; and SEQ ID NO: SEQ ID NO: 9550; SEQ ID NO: 9551; SEQ ID NO: 9552; SEQ ID NO: 9553 and SEQ ID NO: 9554, or a complement thereof.  
     [0066] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in carbohydrate metabolism and transport encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 800; SEQ ID NO: 801 and SEQ ID NO: 802, or a complement thereof.  
     [0067] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in outer membrane and cell wall formation encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 803; SEQ ID NO: 804; SEQ ID NO: 805; SEQ ID NO: 806; SEQ ID NO: 807; SEQ ID NO: 808; SEQ ID NO: 809; SEQ ID NO: 810; SEQ ID NO: 811; SEQ ID NO: 812; SEQ ID NO: 813; SEQ ID NO: 814; SEQ ID NO: 815; SEQ ID NO: 816; SEQ ID NO: 817; SEQ ID NO: 818; SEQ ID NO: 819; SEQ ID NO: 820; SEQ ID NO: 821; SEQ ID NO: 822; SEQ ID NO: 823; SEQ ID NO: 824; SEQ ID NO: 825; SEQ ID NO: 826; SEQ ID NO: 827; SEQ ID NO: 828; SEQ ID NO: 829; SEQ ID NO: 830; SEQ ID NO: 831; SEQ ID NO: 832; SEQ ID NO: 833; SEQ ID NO: 834; SEQ ID NO: 835; SEQ ID NO: 836; SEQ ID NO: 837; SEQ ID NO: 838; SEQ ID NO: 839; SEQ ID NO: 840; SEQ ID NO: 841; SEQ ID NO: 842; SEQ ID NO: 843; SEQ ID NO: 844; SEQ ID NO: 845; SEQ ID NO: 846; SEQ ID NO: 847; SEQ ID NO: 848; and SEQ ID NO: 9555; SEQ ID NO: 9556; SEQ ID NO: 9557; SEQ ID NO: 9558; SEQ ID NO: 9559 and SEQ ID NO: 9560, or a complement thereof.  
     [0068] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in energy conversion encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 849; SEQ ID NO: 850; SEQ ID NO: 851; SEQ ID NO:852; SEQ ID NO: 853; SEQ ID NO: 854; SEQ ID NO: 855; SEQ ID NO: 856; SEQ ID NO: 857; SEQ ID NO: 858; SEQ ID NO: 859; SEQ ID NO: 860; SEQ ID NO: 861; SEQ ID NO: 862; SEQ ID NO: 863; SEQ ID NO: 864; SEQ ID NO: 865; SEQ ID NO: 866; SEQ ID NO: 867; SEQ ID NO: 868; SEQ ID NO: 869; SEQ ID NO: 870; SEQ ID NO: 871; SEQ ID NO: 872; SEQ ID NO: 873; SEQ ID NO: 874; SEQ ID NO: 875; SEQ ID NO: 876; SEQ ID NO: 877; SEQ ID NO: 878; SEQ ID NO: 879; SEQ ID NO: 880; SEQ ID NO: 881; SEQ ID NO: 882; SEQ ID NO: 883; SEQ ID NO: 884; SEQ ID NO: 885; SEQ ID NO: 886; SEQ ID NO: 887; SEQ ID NO: 888; SEQ ID NO: 889; SEQ ID NO: 890; SEQ ID NO: 891; SEQ ID NO: 892; SEQ ID NO: 893; SEQ ID NO: 894; SEQ ID NO: 895; SEQ ID NO: 896; SEQ ID NO: 897; SEQ ID NO: 898; SEQ ID NO: 899; SEQ ID NO: 900; SEQ ID NO: 901; SEQ ID NO: 902; SEQ ID NO: 903; SEQ ID NO: 904; SEQ ID NO: 905; SEQ ID NO: 906; SEQ ID NO: 907; SEQ ID NO: 908; SEQ ID NO: 909; SEQ ID NO: 910; SEQ ID NO: 911; SEQ ID NO: 912; SEQ ID NO: 913; SEQ ID NO: 914; SEQ ID NO: 915; SEQ ID NO: 916; SEQ ID NO: 917; SEQ ID NO: 918; SEQ ID NO: 919; SEQ ID NO: 920; SEQ ID NO: 921; SEQ ID NO: 922; SEQ ID NO: 923; SEQ ID NO: 924; SEQ ID NO: 925; SEQ ID NO: 926; SEQ ID NO: 927; SEQ ID NO: 928; SEQ ID NO: 929; SEQ ID NO: 930; SEQ ID NO: 931; SEQ ID NO: 932; SEQ ID NO: 933; SEQ ID NO: 934; SEQ ID NO: 935; SEQ ID NO: 936; SEQ ID NO: 937; SEQ ID NO: 938; SEQ ID NO: 939; SEQ ID NO: 940; SEQ ID NO: 941; SEQ ID NO: 942; SEQ ID NO: 943; SEQ ID NO: 944; SEQ ID NO: 945; SEQ ID NO: 946; SEQ ID NO: 947; SEQ ID NO: 948; SEQ ID NO: 949; SEQ ID NO: 950; SEQ ID NO: 951; SEQ ID NO: 952; SEQ ID NO: 953; SEQ ID NO: 954; SEQ ID NO: 955; SEQ ID NO: 956; SEQ ID NO: 957; SEQ ID NO: 958; SEQ ID NO: 959; SEQ ID NO: 960; SEQ ID NO: 961; SEQ ID NO: 962; SEQ ID NO: 963; SEQ ID NO: 964; SEQ ID NO: 965; SEQ ID NO: 966; SEQ ID NO: 967; SEQ ID NO: 968; SEQ ID NO: 969; SEQ ID NO: 970; SEQ ID NO: 971; SEQ ID NO: 972; SEQ ID NO: 973; SEQ ID NO: 974; SEQ ID NO: 975; SEQ ID NO: 976; SEQ ID NO: 977; SEQ ID NO: 978; SEQ ID NO: 979; SEQ ID NO: 980; SEQ ID NO: 981; SEQ ID NO: 982; SEQ ID NO: 983; SEQ ID NO: 984; SEQ ID NO: 985; SEQ ID NO: 986; SEQ ID NO: 987; SEQ ID NO: 988; SEQ ID NO: 989; SEQ ID NO: 990; SEQ ID NO: 991; SEQ ID NO: 992; SEQ ID NO: 993; SEQ ID NO: 994; SEQ ID NO: 995 and SEQ ID NO: 9561; SEQ ID NO: 9562; SEQ ID NO: 9563; SEQ ID NO: 9564 and SEQ ID NO: 9565, or a complement thereof.  
     [0069] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in secretion and adhesion encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 996; SEQ ID NO: 997; SEQ ID NO: 998; SEQ ID NO: 999; SEQ ID NO: 1000; SEQ ID NO: 1001; SEQ ID NO: 1002; SEQ ID NO: 1003; SEQ ID NO: 1004; SEQ ID NO: 1005; SEQ ID NO: 1006; SEQ ID NO: 1007 and SEQ ID NO: 9566, or a complement thereof.  
     [0070] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in regulation encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1008; SEQ ID NO: 1009; SEQ ID NO: 1010; SEQ ID NO: 1011; SEQ ID NO: 1012; SEQ ID NO: 1013; SEQ ID NO: 1014; SEQ ID NO: 1015; SEQ ID NO: 1016; SEQ ID NO: 1017; SEQ ID NO: 1018; SEQ ID NO: 1019; SEQ ID NO: 1020; SEQ ID NO: 1021; SEQ ID NO: 1022; SEQ ID NO: 1023; SEQ ID NO: 1024; SEQ ID NO: 1025; SEQ ID NO: 1026 and SEQ ID NO: 1027, or a complement thereof.  
     [0071] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane chaperone polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1028; SEQ ID NO: 1029; SEQ ID NO: 1030; SEQ ID NO: 1031 and SEQ ID NO: 9567, or a complement thereof.  
     [0072] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in cell division encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1032; SEQ ID NO: 1033; SEQ ID NO: 1034; SEQ ID NO: 1035; SEQ ID NO: 1036; SEQ ID NO: 1037; SEQ ID NO: 1038; SEQ ID NO: 1039; SEQ ID NO: 1040; SEQ ID NO: 1041; SEQ ID NO: 1042; SEQ ID NO: 1043; SEQ ID NO: 1044; SEQ ID NO: 1045; SEQ ID NO: 1046; SEQ ID NO: 1047; SEQ ID NO: 1048; SEQ ID NO: 1049; SEQ ID NO: 1050; SEQ ID NO: 1051; SEQ ID NO: 1052; and SEQ ID NO: 9568 and SEQ ID NO: 9569, or a complement thereof.  
     [0073] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in motility encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1053 and SEQ ID NO: 1054, or a complement thereof.  
     [0074] Particularly preferred is an isolated nucleic acid having a nucleotide sequence encoding an  H. pylori  cytoplasmic polypeptide or a fragment thereof, the nucleic acid selected from the group consisting of SEQ ID NO: 1576; SEQ ID NO: 1577; SEQ ID NO: 1578; SEQ ID NO: 1579; SEQ ID NO: 1580; SEQ ID NO: 1581; SEQ ID NO: 1582; SEQ ID NO: 1583; SEQ ID NO: 1584; SEQ ID NO: 1585; SEQ ID NO: 1586; SEQ ID NO: 1587; SEQ ID NO: 1588; SEQ ID NO: 1589; SEQ ID NO: 1590; SEQ ID NO: 1591; SEQ ID NO: 1592; SEQ ID NO: 1593; SEQ ID NO: 1594; SEQ ID NO: 1595; SEQ ID NO: 1596; SEQ ID NO: 1597; SEQ ID NO: 1598; SEQ ID NO: 1599; SEQ ID NO: 1600; SEQ ID NO: 1601; SEQ ID NO: 1602; SEQ ID NO: 1603; SEQ ID NO: 1604; SEQ ID NO: 1605; SEQ ID NO: 1606; SEQ ID NO: 1607; SEQ ID NO: 1608; SEQ ID NO: 1609; SEQ ID NO: 1610; SEQ ID NO: 1611; SEQ ID NO: 1612; SEQ ID NO: 1613; SEQ ID NO: 1614; SEQ ID NO: 1615; SEQ ID NO: 1616; SEQ ID NO: 1617; SEQ ID NO: 1618; SEQ ID NO: 1619; SEQ ID NO: 1620; SEQ ID NO: 1621; SEQ ID NO: 1622; SEQ ID NO: 1623; SEQ ID NO: 1624; SEQ ID NO: 1625; SEQ ID NO: 1626; SEQ ID NO: 1627; SEQ ID NO: 1628; SEQ ID NO: 1629; SEQ ID NO: 1630; SEQ ID NO: 1631; SEQ ID NO: 1632; SEQ ID NO: 1633; SEQ ID NO: 1634; SEQ ID NO: 1635; SEQ ID NO: 1636; SEQ ID NO: 1637; SEQ ID NO: 1638; SEQ ID NO: 1639; SEQ ID NO: 1640; SEQ ID NO: 1641; SEQ ID NO: 1642; SEQ ID NO: 1643; SEQ ID NO: 1644; SEQ ID NO: 1645; SEQ ID NO: 1646; SEQ ID NO: 1647; SEQ ID NO: 1648; SEQ ID NO: 1649; SEQ ID NO: 1650; SEQ ID NO: 1651; SEQ ID NO: 1652; SEQ ID NO: 1653; SEQ ID NO: 1654; SEQ ID NO: 1655; SEQ ID NO: 1656; SEQ ID NO: 1657; SEQ ID NO: 1658; SEQ ID NO: 1659; SEQ ID NO: 1660; SEQ ID NO: 1661; SEQ ID NO: 1662; SEQ ID NO: 1663; SEQ ID NO: 1664; SEQ ID NO: 1665; SEQ ID NO: 1666; SEQ ID NO: 1667; SEQ ID NO: 1668; SEQ ID NO: 1669; SEQ ID NO: 1670; SEQ ID NO: 1671; SEQ ID NO: 1672; SEQ ID NO: 1673; SEQ ID NO: 1674; SEQ ID NO: 1675; SEQ ID NO: 1676; SEQ ID NO: 1677; SEQ ID NO: 1678; SEQ ID NO: 1679; SEQ ID NO: 1680; SEQ ID NO: 1681; SEQ ID NO: 1682; SEQ ID NO: 1683; SEQ ID NO: 1684; SEQ ID NO: 1685; SEQ ID NO: 1686; SEQ ID NO: 1687; SEQ ID NO: 1688; SEQ ID NO: 1689; SEQ ID NO: 1690; SEQ ID NO: 1691; SEQ ID NO: 1692; SEQ ID NO: 1693; SEQ ID NO: 1694; SEQ ID NO: 1695; SEQ ID NO: 1696; SEQ ID NO: 1697; SEQ ID NO: 1698; SEQ ID NO: 1699; SEQ ID NO: 1700; SEQ ID NO: 1701; SEQ ID NO: 1702; SEQ ID NO: 1703; SEQ ID NO: 1704; SEQ ID NO: 1705; SEQ ID NO: 1706; SEQ ID NO: 1707; SEQ ID NO: 1708; SEQ ID NO: 1709; SEQ ID NO: 1710; SEQ ID NO: 1711; SEQ ID NO: 1712; SEQ ID NO: 1713; SEQ ID NO: 1714; SEQ ID NO: 1715; SEQ ID NO: 1716; SEQ ID NO: 1717; SEQ ID NO: 1718; SEQ ID NO: 1719; SEQ ID NO: 1720; SEQ ID NO: 1721; SEQ ID NO: 1722; SEQ ID NO: 1723; SEQ ID NO: 1724; SEQ ID NO: 1725; SEQ ID NO: 1726; SEQ ID NO: 1727; SEQ ID NO: 1728; SEQ ID NO: 1729; SEQ ID NO: 1730; SEQ ID NO: 1731; SEQ ID NO: 1732; SEQ ID NO: 1733; SEQ ID NO: 1734; SEQ ID NO: 1735; SEQ ID NO: 1736; SEQ ID NO: 1737; SEQ ID NO: 1738; SEQ ID NO: 1739; SEQ ID NO: 1740; SEQ ID NO: 1741; SEQ ID NO: 1742; SEQ ID NO: 1743; SEQ ID NO: 1744; SEQ ID NO: 1745; SEQ ID NO: 1746; SEQ ID NO: 1747; SEQ ID NO: 1748; SEQ ID NO: 1749; SEQ ID NO: 1750; SEQ ID NO: 1751; SEQ ID NO: 1752; SEQ ID NO: 1753; SEQ ID NO: 1754; SEQ ID NO: 1755; SEQ ID NO: 1756; SEQ ID NO: 1757; SEQ ID NO: 1758; SEQ ID NO: 1759; SEQ ID NO: 1760; SEQ ID NO: 1761; SEQ ID NO: 1762; SEQ ID NO: 1763; SEQ ID NO: 1764; SEQ ID NO: 1765; SEQ ID NO: 1766; SEQ ID NO: 1767; SEQ ID NO: 1768; SEQ ID NO: 1769; SEQ ID NO: 1770; SEQ ID NO: 1771; SEQ ID NO: 1772; SEQ ID NO: 1773; SEQ ID NO: 1774; SEQ ID NO: 1775; SEQ ID NO: 1776; SEQ ID NO: 1777; SEQ ID NO: 1778; SEQ ID NO: 1779; SEQ ID NO: 1780; SEQ ID NO: 1781; SEQ ID NO: 1782; SEQ ID NO: 1783; SEQ ID NO: 1784; SEQ ID NO: 1785; SEQ ID NO: 1786; SEQ ID NO: 1787; SEQ ID NO: 1788; SEQ ID NO: 1789; SEQ ID NO: 1790; SEQ ID NO: 1791; SEQ ID NO: 1792; SEQ ID NO: 1793; SEQ ID NO: 1794; SEQ ID NO: 1795; SEQ ID NO: 1796; SEQ ID NO: 1797; SEQ ID NO: 1798; SEQ ID NO: 1799; SEQ ID NO: 1800; SEQ ID NO: 1801; SEQ ID NO: 1802; SEQ ID NO: 1803; SEQ ID NO: 1804; SEQ ID NO: 1805; SEQ ID NO: 1806; SEQ ID NO: 1807; SEQ ID NO: 1808; SEQ ID NO: 1809; SEQ ID NO: 1810; SEQ ID NO: 1811; SEQ ID NO: 1812; SEQ ID NO: 1813; SEQ ID NO: 1814; SEQ ID NO: 1815; SEQ ID NO: 1816; SEQ ID NO: 1817; SEQ ID NO: 1818; SEQ ID NO: 1819; SEQ ID NO: 1820; SEQ ID NO: 1821; SEQ ID NO: 1822; SEQ ID NO: 1823; SEQ ID NO: 1824; SEQ ID NO: 1825; SEQ ID NO: 1826; SEQ ID NO: 1827; SEQ ID NO: 1828; SEQ ID NO: 1829; SEQ ID NO: 1830; SEQ ID NO: 1831; SEQ ID NO: 1832; SEQ ID NO: 1833; SEQ ID NO: 1834; SEQ ID NO: 1835; SEQ ID NO: 1836; SEQ ID NO: 1837; SEQ ID NO: 1838; SEQ ID NO: 1839; SEQ ID NO: 1840; SEQ ID NO: 1841; SEQ ID NO: 1842; SEQ ID NO: 1843; SEQ ID NO: 1844; SEQ ID NO: 1845; SEQ ID NO: 1846; SEQ ID NO: 1847; SEQ ID NO: 1848; SEQ ID NO: 1849; SEQ ID NO: 1850; SEQ ID NO: 1851; SEQ ID NO: 1852; SEQ ID NO: 1853; SEQ ID NO: 1854; SEQ ID NO: 1855; SEQ ID NO: 1856; SEQ ID NO: 1857; SEQ ID NO: 1858; SEQ ID NO: 1859; SEQ ID NO: 1860; SEQ ID NO: 1861; SEQ ID NO: 1862; SEQ ID NO: 1863; SEQ ID NO: 1864; SEQ ID NO: 1865; SEQ ID NO: 1866; SEQ ID NO: 1867; SEQ ID NO: 1868; SEQ ID NO: 1869; SEQ ID NO: 1870; SEQ ID NO: 1871; SEQ ID NO: 1872; SEQ ID NO: 1873; SEQ ID NO: 1874; SEQ ID NO: 1875; SEQ ID NO: 1876; SEQ ID NO: 1877; SEQ ID NO: 1878; SEQ ID NO: 1879; SEQ ID NO: 1880; SEQ ID NO: 1881; SEQ ID NO: 1882; SEQ ID NO: 1883; SEQ ID NO: 1884; SEQ ID NO: 1885; SEQ ID NO: 1886; SEQ ID NO: 1887; SEQ ID NO: 1888; SEQ ID NO: 1889; SEQ ID NO: 1890; SEQ ID NO: 1891; SEQ ID NO: 1892; SEQ ID NO: 1893; SEQ ID NO: 1894; SEQ ID NO: 1895; SEQ ID NO: 1896; SEQ ID NO: 1897; SEQ ID NO: 1898; SEQ ID NO: 1899; SEQ ID NO: 1900; SEQ ID NO: 1901; SEQ ID NO: 1902; SEQ ID NO: 1903; SEQ ID NO: 1904; SEQ ID NO: 1905; SEQ ID NO: 1906; SEQ ID NO: 1907; SEQ ID NO: 1908; SEQ ID NO: 1909; SEQ ID NO: 1910; SEQ ID NO: 1911; SEQ ID NO: 1912; SEQ ID NO: 1913; SEQ ID NO: 1914; SEQ ID NO: 1915; SEQ ID NO: 1916; SEQ ID NO: 1917; SEQ ID NO: 1918; SEQ ID NO: 1919; SEQ ID NO: 1920; SEQ ID NO: 1921; SEQ ID NO: 1922; SEQ ID NO: 1923; SEQ ID NO: 1924; SEQ ID NO: 1925; SEQ ID NO: 1926; SEQ ID NO: 1927; SEQ ID NO: 1928; SEQ ID NO. 1929; SEQ ID NO: 1930; SEQ ID NO: 1931; SEQ ID NO: 1932; SEQ ID NO: 1933; SEQ ID NO: 1934; SEQ ID NO: 1935; SEQ ID NO: 1936; SEQ ID NO: 1937; SEQ ID NO: 1938; SEQ ID NO: 1939; SEQ ID NO: 1940; SEQ ID NO: 1941; SEQ ID NO: 1942; SEQ ID NO: 1943; SEQ ID NO: 1944; SEQ ID NO: 1945; SEQ ID NO: 1946; SEQ ID NO: 1947; SEQ ID NO: 1948; SEQ ID NO: 1949; SEQ ID NO: 1950; SEQ ID NO: 1951; SEQ ID NO: 1952; SEQ ID NO: 1953; SEQ ID NO: 1954; SEQ ID NO: 1955; SEQ ID NO: 1956; SEQ ID NO: 1957; SEQ ID NO: 1958; SEQ ID NO: 1959; SEQ ID NO: 1960; SEQ ID NO: 1961; SEQ ID NO: 1962; SEQ ID NO: 1963; SEQ ID NO: 1964; SEQ ID NO: 1965; SEQ ID NO: 1966; SEQ ID NO: 1967; SEQ ID NO: 1968; SEQ ID NO: 1969; SEQ ID NO: 1970; SEQ ID NO: 1971; SEQ ID NO: 1972; SEQ ID NO: 1973; SEQ ID NO: 1974; SEQ ID NO: 1975; SEQ ID NO: 1976; SEQ ID NO: 1977; SEQ ID NO: 1978; SEQ ID NO: 1979; SEQ ID NO: 1980; SEQ ID NO: 1981; SEQ ID NO: 1982; SEQ ID NO: 1983; SEQ ID NO: 1984; SEQ ID NO: 1985; SEQ ID NO: 1986; SEQ ID NO: 1987; SEQ ID NO: 1988; SEQ ID NO: 1989; SEQ ID NO: 1990; SEQ ID NO: 1991; SEQ ID NO: 1992; SEQ ID NO: 1993; SEQ ID NO: 1994; SEQ ID NO: 1995; SEQ ID NO: 1996; SEQ ID NO: 1997; SEQ ID NO: 1998; SEQ ID NO: 1999; SEQ ID NO: 2000; SEQ ID NO: 2001; SEQ ID NO: 2002; SEQ ID NO: 2003; SEQ ID NO: 2004; SEQ ID NO: 2005; SEQ ID NO: 2006; SEQ ID NO: 2007; SEQ ID NO: 2008; SEQ ID NO: 2009; SEQ ID NO: 2010; SEQ ID NO: 2011; SEQ ID NO: 2012; SEQ ID NO: 2013; SEQ ID NO: 2014; SEQ ID NO: 2015; SEQ ID NO: 2016; SEQ ID NO: 2017; SEQ ID NO: 2018; SEQ ID NO: 2019; SEQ ID NO: 2020; SEQ ID NO: 2021; SEQ ID NO: 2022; SEQ ID NO: 2023; SEQ ID NO: 2024; SEQ ID NO: 2025; SEQ ID NO: 2026; SEQ ID NO: 2027; SEQ ID NO: 2028; SEQ ID NO: 2029; SEQ ID NO: 2030; SEQ ID NO: 2031; SEQ ID NO: 2032; SEQ ID NO: 2033; SEQ ID NO: 2034; SEQ ID NO: 2035; SEQ ID NO: 2036; SEQ ID NO: 2037; SEQ ID NO: 2038; SEQ ID NO: 2039; SEQ ID NO: 2040; SEQ ID NO: 2041; SEQ ID NO: 2042; SEQ ID NO: 2043; SEQ ID NO: 2044; SEQ ID NO: 2045; SEQ ID NO: 2046; SEQ ID NO: 2047; SEQ ID NO: 2048; SEQ ID NO: 2049; SEQ ID NO: 2050; SEQ ID NO: 2051; SEQ ID NO: 2052; SEQ ID NO: 2053; SEQ ID NO: 2054; SEQ ID NO: 2055; SEQ ID NO: 2056; SEQ ID NO: 2057; SEQ ID NO: 2058; SEQ ID NO: 2059; SEQ ID NO: 2060; SEQ ID NO: 2061; SEQ ID NO: 2062; SEQ ID NO: 2063; SEQ ID NO: 2064; SEQ ID NO: 2065; SEQ ID NO: 2066; SEQ ID NO: 2067; SEQ ID NO: 2068; SEQ ID NO: 2069; SEQ ID NO: 2070; SEQ ID NO: 2071; SEQ ID NO: 2072; SEQ ID NO: 2073; SEQ ID NO: 2074; SEQ ID NO: 2075; SEQ ID NO: 2076; SEQ ID NO: 2077; SEQ ID NO: 2078; SEQ ID NO: 2079; SEQ ID NO: 2080; SEQ ID NO: 2081; SEQ ID NO: 2082; SEQ ID NO: 2083; SEQ ID NO: 2084; SEQ ID NO: 2085; SEQ ID NO: 2086; SEQ ID NO: 2087; SEQ ID NO: 2088; SEQ ID NO: 2089; SEQ ID NO: 2090; SEQ ID NO: 2091; SEQ ID NO: 2092; SEQ ID NO: 2093; SEQ ID NO: 2094; SEQ ID NO: 2095; SEQ ID NO: 2096; SEQ ID NO: 2097; SEQ ID NO: 2098; SEQ ID NO: 2099; SEQ ID NO: 2100; SEQ ID NO: 2101; SEQ ID NO: 2102; SEQ ID NO: 2103; SEQ ID NO: 2104; SEQ ID NO: 2105; SEQ ID NO: 2106; SEQ ID NO: 2107; SEQ ID NO: 2108; SEQ ID NO: 2109; SEQ ID NO: 2110; SEQ ID NO: 2111; SEQ ID NO: 2112; SEQ ID NO: 2113; SEQ ID NO: 2114; SEQ ID NO: 2115; SEQ ID NO: 2116; SEQ ID NO: 2117; SEQ ID NO: 2118; SEQ ID NO: 2119; SEQ ID NO: 2120; SEQ ID NO: 2121; SEQ ID NO: 2122; SEQ ID NO: 2123; SEQ ID NO: 2124; SEQ ID NO: 2125; SEQ ID NO: 2126; SEQ ID NO: 2127; SEQ ID NO: 2128; SEQ ID NO: 2129; SEQ ID NO: 2130; SEQ ID NO: 2131; SEQ ID NO: 2132; SEQ ID NO: 2133; SEQ ID NO: 2134; SEQ ID NO: 2135; SEQ ID NO: 2136; SEQ ID NO: 2137; SEQ ID NO: 2138; SEQ ID NO: 2139; SEQ ID NO: 2140; SEQ ID NO: 2141; SEQ ID NO: 2142; SEQ ID NO: 2143; SEQ ID NO: 2144; SEQ ID NO: 2145; SEQ ID NO: 2146; SEQ ID NO: 2147; SEQ ID NO: 2148; SEQ ID NO: 2149; SEQ ID NO: 2150; SEQ ID NO: 2151; SEQ ID NO: 2152; SEQ ID NO: 2153; SEQ ID NO: 2154; SEQ ID NO: 2155; SEQ ID NO: 2156; SEQ ID NO: 2157; SEQ ID NO: 2158; SEQ ID NO: 2159; SEQ ID NO: 2160; SEQ ID NO: 2161; SEQ ID NO: 2162; SEQ ID NO: 2163; SEQ ID NO: 2164; SEQ ID NO: 2165; SEQ ID NO: 2166; SEQ ID NO: 2167; SEQ ID NO: 2168; SEQ ID NO: 2169; SEQ ID NO: 2170; SEQ ID NO: 2171; SEQ ID NO: 2172; SEQ ID NO: 2173; SEQ ID NO: 2174; SEQ ID NO: 2175; SEQ ID NO: 2176; SEQ ID NO: 2177; SEQ ID NO: 2178; SEQ ID NO: 2179; SEQ ID NO: 2180; SEQ ID NO: 2181; SEQ ID NO: 2182; SEQ ID NO: 2183; SEQ ID NO: 2184; SEQ ID NO: 2185; SEQ ID NO: 2186; SEQ ID NO: 2187; SEQ ID NO: 2188; SEQ ID NO: 2189; SEQ ID NO: 2190; SEQ ID NO: 2191; SEQ ID NO: 2192; SEQ ID NO: 2193; SEQ ID NO: 2194; SEQ ID NO: 2195; SEQ ID NO: 2196; SEQ ID NO: 2197; SEQ ID NO: 2198; SEQ ID NO: 2199; SEQ ID NO: 2200; SEQ ID NO: 2201; SEQ ID NO: 2202; SEQ ID NO: 2203; SEQ ID NO: 2204; SEQ ID NO: 2205; SEQ ID NO: 2206; SEQ ID NO: 2207; SEQ ID NO: 2208; SEQ ID NO: 2209; SEQ ID NO: 2210; SEQ ID NO: 2211; SEQ ID NO: 2212; SEQ ID NO: 2213; SEQ ID NO: 2214; SEQ ID NO: 2215; SEQ ID NO: 2216; SEQ ID NO: 2217; SEQ ID NO: 2218; SEQ ID NO: 2219; SEQ ID NO: 2220; SEQ ID NO: 2221; SEQ ID NO: 2222; SEQ ID NO: 2223; SEQ ID NO: 2224; SEQ ID NO: 2225; SEQ ID NO: 2226; SEQ ID NO: 2227; SEQ ID NO: 2228; SEQ ID NO: 2229; SEQ ID NO: 2230; SEQ ID NO: 2231; SEQ ID NO: 2232; SEQ ID NO: 2233; SEQ ID NO: 2234; SEQ ID NO: 2235; SEQ ID NO: 2236; SEQ ID NO: 2237; SEQ ID NO: 2238; SEQ ID NO: 2239; SEQ ID NO: 2240; SEQ ID NO: 2241; SEQ ID NO: 2242; SEQ ID NO: 2243; SEQ ID NO: 2244; SEQ ID NO: 2245; SEQ ID NO: 2246; SEQ ID NO: 2247; SEQ ID NO: 2248; SEQ ID NO: 2249; SEQ ID NO: 2250; SEQ ID NO: 2251; SEQ ID NO: 2252; SEQ ID NO: 2253; SEQ ID NO: 2254; SEQ ID NO: 2255; SEQ ID NO: 2256; SEQ ID NO: 2257; SEQ ID NO: 2258; SEQ ID NO: 2259; SEQ ID NO: 2260; SEQ ID NO: 2261; SEQ ID NO: 2262; SEQ ID NO: 2263; SEQ ID NO: 2264; SEQ ID NO: 2265; SEQ ID NO: 2266; SEQ ID NO: 2267; SEQ ID NO: 2268; SEQ ID NO: 2269; SEQ ID NO: 2270; SEQ ID NO: 2271; SEQ ID NO: 2272; SEQ ID NO: 2273; SEQ ID NO: 2274; SEQ ID NO: 2275; SEQ ID NO: 2276; SEQ ID NO: 2277; SEQ ID NO: 2278; SEQ ID NO: 2279; SEQ ID NO: 2280; SEQ ID NO: 2281; SEQ ID NO: 2282; SEQ ID NO: 2283; SEQ ID NO: 2284; SEQ ID NO: 2285; SEQ ID NO: 2286; SEQ ID NO: 2287; SEQ ID NO: 2288; SEQ ID NO: 2289; SEQ ID NO: 2290; SEQ ID NO: 2291; SEQ ID NO: 2292; SEQ ID NO:2293; SEQ ID NO:2294; SEQ ID NO:2295; SEQ ID NO:2296; SEQ ID NO: 2297; SEQ ID NO: 2298; SEQ ID NO: 2299; SEQ ID NO: 2300; SEQ ID NO: 2301; SEQ ID NO: 2302; SEQ ID NO: 2303; SEQ ID NO: 2304; SEQ ID NO: 2305; SEQ ID NO: 2306; SEQ ID NO: 2307; SEQ ID NO: 2308; SEQ ID NO: 2309; SEQ ID NO: 2310; SEQ ID NO: 2311; SEQ ID NO: 2312; SEQ ID NO: 2313; SEQ ID NO: 2314; SEQ ID NO: 2315; SEQ ID NO: 2316; SEQ ID NO: 2317; SEQ ID NO: 2318; SEQ ID NO: 2319; SEQ ID NO: 2320; SEQ ID NO: 2321; SEQ ID NO: 2322; SEQ ID NO: 2323; SEQ ID NO: 2324; SEQ ID NO: 2325; SEQ ID NO: 2326; SEQ ID NO: 2327; SEQ ID NO: 2328; SEQ ID NO: 2329; SEQ ID NO: 2330; SEQ ID NO: 2331; SEQ ID NO: 2332; SEQ ID NO: 2333; SEQ ID NO: 2334; SEQ ID NO: 2335; SEQ ID NO: 2336; SEQ ID NO: 2337; SEQ ID NO: 2338; SEQ ID NO: 2339; SEQ ID NO: 2340; SEQ ID NO: 2341; SEQ ID NO: 2342; SEQ ID NO: 2343; SEQ ID NO: 2344; SEQ ID NO: 2345; SEQ ID NO: 2346; SEQ ID NO: 2347; SEQ ID NO: 2348; SEQ ID NO: 2349; SEQ ID NO: 2350; SEQ ID NO: 2351; SEQ ID NO: 2352; SEQ ID NO: 2353; SEQ ID NO: 2354; SEQ ID NO: 2355; SEQ ID NO: 2356; SEQ ID NO: 2357; SEQ ID NO: 2358; SEQ ID NO: 2359; SEQ ID NO: 2360; SEQ ID NO: 2361; SEQ ID NO: 2362; SEQ ID NO: 2363; SEQ ID NO: 2364; SEQ ID NO: 2365; SEQ ID NO: 2366; SEQ ID NO: 2367; SEQ ID NO: 2368; SEQ ID NO: 2369; SEQ ID NO: 2370; SEQ ID NO: 2371; SEQ ID NO: 2372; SEQ ID NO: 2373; SEQ ID NO: 2374; SEQ ID NO: 2375; SEQ ID NO: 2376; SEQ ID NO: 2377; SEQ ID NO: 2378; SEQ ID NO: 2379; SEQ ID NO: 2380; SEQ ID NO: 2381; SEQ ID NO: 2382; SEQ ID NO: 2383; SEQ ID NO: 2384; SEQ ID NO: 2385; SEQ ID NO: 2386; SEQ ID NO: 2387; SEQ ID NO: 2388; SEQ ID NO: 2389; SEQ ID NO: 2390; SEQ ID NO: 2391; SEQ ID NO: 2392; SEQ ID NO: 2393; SEQ ID NO: 2394; SEQ ID NO: 2395; SEQ ID NO: 2396; SEQ ID NO: 2397; SEQ ID NO: 2398; SEQ ID NO: 2399; SEQ ID NO: 2400; SEQ ID NO: 2401; SEQ ID NO: 2402; SEQ ID NO: 2403; SEQ ID NO: 2404; SEQ ID NO: 2405; SEQ ID NO: 2406; SEQ ID NO: 2407; SEQ ID NO: 2408; SEQ ID NO: 2409; SEQ ID NO: 2410; SEQ ID NO: 2411; SEQ ID NO: 2412; SEQ ID NO: 2413; SEQ ID NO: 2414; SEQ ID NO: 2415; SEQ ID NO: 2416; SEQ ID NO: 2417; SEQ ID NO: 2418; SEQ ID NO: 2419; SEQ ID NO: 2420; SEQ ID NO: 2421; SEQ ID NO: 2422; SEQ ID NO: 2423; SEQ ID NO: 2424; SEQ ID NO: 2425; SEQ ID NO: 2426; SEQ ID NO: 2427; SEQ ID NO: 2428; SEQ ID NO: 2429; SEQ ID NO: 2430; SEQ ID NO: 2431; SEQ ID NO: 2432; SEQ ID NO: 2433; SEQ ID NO: 2434; SEQ ID NO: 2435; SEQ ID NO: 2436; SEQ ID NO: 2437; SEQ ID NO: 2438; SEQ ID NO: 2439; SEQ ID NO: 2440; SEQ ID NO: 2441; SEQ ID NO: 2442; SEQ ID NO: 2443; SEQ ID NO: 2444; SEQ ID NO: 2445; SEQ ID NO: 2446; SEQ ID NO: 2447; SEQ ID NO: 2448; SEQ ID NO: 2449; SEQ ID NO: 2450; SEQ ID NO: 2451; SEQ ID NO: 2452; SEQ ID NO: 2453; SEQ ID NO: 2454; SEQ ID NO: 2455; SEQ ID NO: 2456; SEQ ID NO: 2457; SEQ ID NO: 2458; SEQ ID NO: 2459; SEQ ID NO: 2460; SEQ ID NO: 2461; SEQ ID NO: 2462; SEQ ID NO: 2463; SEQ ID NO: 2464; SEQ ID NO: 2465; SEQ ID NO: 2466; SEQ ID NO: 2467; SEQ ID NO: 2468; SEQ ID NO: 2469; SEQ ID NO: 2470; SEQ ID NO: 2471; SEQ ID NO: 2472; SEQ ID NO: 2473; SEQ ID NO: 2474; SEQ ID NO: 2475; SEQ ID NO: 2476; SEQ ID NO: 2477; SEQ ID NO: 2478; SEQ ID NO: 2479; SEQ ID NO: 2480; SEQ ID NO: 2481; SEQ ID NO: 2482; SEQ ID NO: 2483; SEQ ID NO: 2484; SEQ ID NO: 2485; SEQ ID NO: 2486; SEQ ID NO: 2487; SEQ ID NO: 2488; SEQ ID NO: 2489; SEQ ID NO: 2490; SEQ ID NO: 2491; SEQ ID NO: 2492; SEQ ID NO: 2493; SEQ ID NO: 2494; SEQ ID NO: 2495; SEQ ID NO: 2496; SEQ ID NO: 2497; SEQ ID NO: 2498; SEQ ID NO: 2499; SEQ ID NO: 2500; SEQ ID NO: 2501; SEQ ID NO: 2502; SEQ ID NO: 2503; SEQ ID NO: 2504; SEQ ID NO: 2505; SEQ ID NO: 2506; SEQ ID NO: 2507; SEQ ID NO: 2508; SEQ ID NO: 2509; SEQ ID NO: 2510; SEQ ID NO: 2511; SEQ ID NO: 2512; SEQ ID NO: 2513; SEQ ID NO: 2514; SEQ ID NO: 2515; SEQ ID NO: 2516; SEQ ID NO: 2517; SEQ ID NO: 2518; SEQ ID NO: 2519; SEQ ID NO: 2520; SEQ ID NO: 2521; SEQ ID NO: 2522; SEQ ID NO: 2523; SEQ ID NO: 2524; SEQ ID NO: 2525; SEQ ID NO: 2526; SEQ ID NO: 2527; SEQ ID NO: 2528; SEQ ID NO: 2529; SEQ ID NO: 2530; SEQ ID NO: 2531; SEQ ID NO: 2532; SEQ ID NO: 2533; SEQ ID NO: 2534; SEQ ID NO: 2535; SEQ ID NO: 2536; SEQ ID NO: 2537; SEQ ID NO: 2538; SEQ ID NO: 2539; SEQ ID NO: 2540; SEQ ID NO: 2541; SEQ ID NO: 2542; SEQ ID NO: 2543; SEQ ID NO: 2544; SEQ ID NO: 2545; SEQ ID NO: 2546; SEQ ID NO: 2547; SEQ ID NO: 2548; SEQ ID NO: 2549; SEQ ID NO: 2550; SEQ ID NO: 2551; SEQ ID NO: 2552; SEQ ID NO: 2553; SEQ ID NO: 2554; SEQ ID NO: 2555; SEQ ID NO: 2556; SEQ ID NO: 2557; SEQ ID NO: 2558; SEQ ID NO: 2559; SEQ ID NO: 2560; SEQ ID NO: 2561; SEQ ID NO: 2562; SEQ ID NO: 2563; SEQ ID NO: 2564; SEQ ID NO: 2565; SEQ ID NO: 2566; SEQ ID NO: 2567; SEQ ID NO: 2568; SEQ ID NO: 2569; SEQ ID NO: 2570; SEQ ID NO: 2571; SEQ ID NO: 2572; SEQ ID NO: 2573; SEQ ID NO: 2574; SEQ ID NO: 2575; SEQ ID NO: 2576; SEQ ID NO: 2577; SEQ ID NO: 2578; SEQ ID NO: 2579; SEQ ID NO: 2580; SEQ ID NO: 2581; SEQ ID NO: 2582; SEQ ID NO: 2583; SEQ ID NO: 2584; SEQ ID NO: 2585; SEQ ID NO: 2586; SEQ ID NO: 2587; SEQ ID NO: 2588; SEQ ID NO: 2589; SEQ ID NO: 2590; SEQ ID NO: 2591; SEQ ID NO: 2592; SEQ ID NO: 2593; SEQ ID NO: 2594; SEQ ID NO: 2595; SEQ ID NO: 2596; SEQ ID NO: 2597; SEQ ID NO: 2598; SEQ ID NO: 2599; SEQ ID NO: 2600; SEQ ID NO: 2601; SEQ ID NO: 2602; SEQ ID NO: 2603; SEQ ID NO: 2604; SEQ ID NO: 2605; SEQ ID NO: 2606; SEQ ID NO: 2607; SEQ ID NO: 2608; SEQ ID NO: 2609; SEQ ID NO: 2610; SEQ ID NO: 2611; SEQ ID NO: 2612; SEQ ID NO: 2613; SEQ ID NO: 2614; SEQ ID NO: 2615; SEQ ID NO: 2616; SEQ ID NO: 2617; SEQ ID NO: 2618; SEQ ID NO: 2619; SEQ ID NO: 2620; SEQ ID NO: 2621; SEQ ID NO: 2622; SEQ ID NO: 2623; SEQ ID NO: 2624; SEQ ID NO: 2625; SEQ ID NO: 2626; SEQ ID NO: 2627; SEQ ID NO: 2628; SEQ ID NO: 2629; SEQ ID NO: 2630; SEQ ID NO: 2631; SEQ ID NO: 2632; SEQ ID NO: 2633; SEQ ID NO: 2634; SEQ ID NO: 2635; SEQ ID NO: 2636; SEQ ID NO: 2637; SEQ ID NC: 638; SEQ ID NO: 2639; SEQ ID NO: 2640; SEQ ID NO: 2641; SEQ ID NO: 2642; SEQ ID NO: 2643; SEQ ID NO: 2644; SEQ ID NO: 2645; SEQ ID NO: 2646; SEQ ID NO: 2647; SEQ ID NO: 2648; SEQ ID NO: 2649; SEQ ID NO: 2650; SEQ ID NO: 2651; SEQ ID NO: 2652; SEQ ID NO: 2653; SEQ ID NO: 2654; SEQ ID NO: 2655; SEQ ID NO: 2656; SEQ ID NO: 2657; SEQ ID NO: 2658; SEQ ID NO: 2659; SEQ ID NO: 2660; SEQ ID NO: 2661; SEQ ID NO: 2662; SEQ ID NO: 2663; SEQ ID NO: 2664; SEQ ID NO: 2665; SEQ ID NO: 2666; SEQ ID NO: 2667; SEQ ID NO: 2668; SEQ ID NO: 2669; SEQ ID NO: 2670; SEQ ID NO: 2671; SEQ ID NO: 2672; SEQ ID NO: 2673; SEQ ID NO: 2674; SEQ ID NO: 2675; SEQ ID NO: 2676; SEQ ID NO: 2677; SEQ ID NO: 2678; SEQ ID NO: 2679; SEQ ID NO: 2680; SEQ ID NO: 2681; SEQ ID NO: 2682; SEQ ID NO: 2683; SEQ ID NO: 2684; SEQ ID NO: 2685; SEQ ID NO: 2686; SEQ ID NO: 2687; SEQ ID NO: 2688; SEQ ID NO: 2689; SEQ ID NO: 2690; SEQ ID NO: 2691; SEQ ID NO: 2692; SEQ ID NO: 2693; SEQ ID NO: 2694; SEQ ID NO: 2695; SEQ ID NO: 2696; SEQ ID NO: 2697; SEQ ID NO: 2698; SEQ ID NO: 2699; SEQ ID NO: 2700; SEQ ID NO: 2701; SEQ ID NO: 2702; SEQ ID NO: 2703; SEQ ID NO: 2704; SEQ ID NO: 2705; SEQ ID NO: 2706; SEQ ID NO: 2707; SEQ ID NO: 2708; SEQ ID NO: 2709; SEQ ID NO: 2710; SEQ ID NO: 2711; SEQ ID NO: 2712; SEQ ID NO: 2713; SEQ ID NO: 2714; SEQ ID NO: 2715; SEQ ID NO: 2716; SEQ ID NO: 2717; SEQ ID NO: 2718; SEQ ID NO: 2719; SEQ ID NO: 2720; SEQ ID NO: 2721; SEQ ID NO: 2722; SEQ ID NO: 2723; SEQ ID NO: 2724; SEQ ID NO: 2725; SEQ ID NO: 2726; SEQ ID NO: 2727; SEQ ID NO: 2728; SEQ ID NO: 2729; SEQ ID NO: 2730; SEQ ID NO: 2731; SEQ ID NO: 2732; SEQ ID NO: 2733; SEQ ID NO: 2734; SEQ ID NO: 2735; SEQ ID NO: 2736; SEQ ID NO: 2737; SEQ ID NO: 2738; SEQ ID NO: 2739; SEQ ID NO: 2740; SEQ ID NO: 2741; SEQ ID NO: 2742; SEQ ID NO: 2743; SEQ ID NO: 2744; SEQ ID NO: 2745; SEQ ID NO: 2746; SEQ ID NO: 2747; SEQ ID NO: 2748; SEQ ID NO: 2749; SEQ ID NO: 2750; SEQ ID NO: 2751; SEQ ID NO: 2752; SEQ ID NO: 2753; SEQ ID NO: 2754; SEQ ID NO: 2755; SEQ ID NO: 2756; SEQ ID NO: 2757; SEQ ID NO: 2758; SEQ ID NO: 2759; SEQ ID NO: 2760; SEQ ID NO: 2761; SEQ ID NO: 2762; SEQ ID NO: 2763; SEQ ID NO: 2764; SEQ ID NO: 2765; SEQ ID NO: 2766; SEQ ID NO: 2767; SEQ ID NO: 2768; SEQ ID NO: 2769; SEQ ID NO: 2770; SEQ ID NO: 2771; SEQ ID NO: 2772; SEQ ID NO: 2773; SEQ ID NO: 2774; SEQ ID NO: 2775; SEQ ID NO: 2776; SEQ ID NO: 2777; SEQ ID NO: 2778; SEQ ID NO: 2779; SEQ ID NO: 2780; SEQ ID NO: 2781; SEQ ID NO: 2782; SEQ ID NO: 2783; SEQ ID NO: 2784; SEQ ID NO: 2785; SEQ ID NO: 2786; SEQ ID NO: 2787; SEQ ID NO: 2788; SEQ ID NO: 2789; SEQ ID NO: 2790; SEQ ID NO: 2791; SEQ ID NO: 2792; SEQ ID NO: 2793; SEQ ID NO: 2794; SEQ ID NO: 2795; SEQ ID NO: 2796; SEQ ID NO: 2797; SEQ ID NO: 2798; SEQ ID NO: 2799; SEQ ID NO: 2800; SEQ ID NO: 2081; SEQ ID NO: 2802; SEQ ID NO: 2803; SEQ ID NO: 2804; SEQ ID NO: 2805; SEQ ID NO: 2806; SEQ ID NO: 2807; SEQ ID NO: 2808; SEQ ID NO: 2809; SEQ ID NO: 2810; SEQ ID NO: 2811; SEQ ID NO: 2812; SEQ ID NO: 2813; SEQ ID NO: 2814; SEQ ID NO: 2815; SEQ ID NO: 2816; SEQ ID NO: 2817; SEQ ID NO: 2818; SEQ ID NO: 2819; SEQ ID NO: 2820; SEQ ID NO: 2821; SEQ ID NO: 2822; SEQ ID NO: 2823; SEQ ID NO: 2824; SEQ ID NO: 2825; SEQ ID NO: 2826; SEQ ID NO: 2827; SEQ ID NO: 2828; SEQ ID NO: 2829; SEQ ID NO: 2830; SEQ ID NO: 2831; SEQ ID NO: 2832; SEQ ID NO: 2833; SEQ ID NO: 2834; SEQ ID NO: 2835; SEQ ID NO: 2836; SEQ ID NO: 2837; SEQ ID NO: 2838; SEQ ID NO: 2839; SEQ ID NO: 2840; SEQ ID NO: 2841; SEQ ID NO: 2842; SEQ ID NO: 2843; SEQ ID NO: 2844; SEQ ID NO: 2845; SEQ ID NO: 2846; SEQ ID NO: 2847; SEQ ID NO: 2848; SEQ ID NO: 2849; SEQ ID NO: 2850; SEQ ID NO: 2851; SEQ ID NO: 2852; SEQ ID NO: 2853; SEQ ID NO: 2854; SEQ ID NO: 2855; SEQ ID NO: 2856; SEQ ID NO: 2857; SEQ ID NO: 2858; SEQ ID NO: 2859; SEQ ID NO: 2860; SEQ ID NO: 2861; SEQ ID NO: 2862; SEQ ID NO: 2863; SEQ ID NO: 2864; SEQ ID NO: 2865; SEQ ID NO: 2866; SEQ ID NO: 2867; SEQ ID NO: 2868; SEQ ID NO: 2869; SEQ ID NO: 2870; SEQ ID NO: 2871; SEQ ID NO: 2872; SEQ ID NO: 2873; SEQ ID NO: 2874; SEQ ID NO: 2875; SEQ ID NO: 2876; SEQ ID NO: 2877; SEQ ID NO: 2878; SEQ ID NO: 2879; SEQ ID NO: 2880; SEQ ID NO: 2881; SEQ ID NO: 2882; SEQ ID NO: 2883; SEQ ID NO: 2884; SEQ ID NO: 2885; SEQ ID NO: 2886; SEQ ID NO: 2887; SEQ ID NO: 2888; SEQ ID NO: 2889; SEQ ID NO: 2890; SEQ ID NO: 2891; SEQ ID NO: 2892; SEQ ID NO: 2893; SEQ ID NO: 2894; SEQ ID NO: 2895; SEQ ID NO: 2896; SEQ ID NO: 2897; SEQ ID NO: 2898; SEQ ID NO: 2899; SEQ ID NO: 2900; SEQ ID NO: 2901; SEQ ID NO: 2902; SEQ ID NO: 2903; SEQ ID NO: 2904; SEQ ID NO: 2905; SEQ ID NO: 2906; SEQ ID NO: 2907; SEQ ID NO: 2908; SEQ ID NO: 2909; SEQ ID NO: 2910; SEQ ID NO: 2911; SEQ ID NO: 2912; SEQ ID NO: 2913; SEQ ID NO: 2914; SEQ ID NO: 2915; SEQ ID NO: 2916; SEQ ID NO: 2917; SEQ ID NO: 2918; SEQ ID NO: 2919; SEQ ID NO: 2920; SEQ ID NO: 2921; SEQ ID NO: 2922; SEQ ID NO: 2923; SEQ ID NO: 2924; SEQ ID NO: 2925; SEQ ID NO: 2926; SEQ ID NO: 2927; SEQ ID NO: 2928; SEQ ID NO: 2929; SEQ ID NO: 2930; SEQ ID NO: 2931; SEQ ID NO: 2932; SEQ ID NO: 2933; SEQ ID NO: 2934; SEQ ID NO: 2935; SEQ ID NO: 2936; SEQ ID NO: 2937; SEQ ID NO: 2938; SEQ ID NO: 2939; SEQ ID NO: 2940; SEQ ID NO: 2941; SEQ ID NO: 2942; SEQ ID NO: 2943; SEQ ID NO: 2944; SEQ ID NO: 2945; SEQ ID NO: 2946; SEQ ID NO: 2947; SEQ ID NO: 2948; SEQ ID NO: 2949; SEQ ID NO: 2950; SEQ ID NO: 2951; SEQ ID NO: 2952; SEQ ID NO: 2953; SEQ ID NO: 2954; SEQ ID NO: 2955; SEQ ID NO: 2956; SEQ ID NO: 2957; SEQ ID NO: 2958; SEQ ID NO: 2959; SEQ ID NO: 2960; SEQ ID NO: 2961; SEQ ID NO: 2962; SEQ ID NO: 2963; SEQ ID NO: 2964; SEQ ID NO: 2965; SEQ ID NO: 2966; SEQ ID NO: 2967; SEQ ID NO: 2968; SEQ ID NO: 2969; SEQ ID NO: 2970; SEQ ID NO: 2971; SEQ ID NO: 2972; SEQ ID NO: 2973; SEQ ID NO: 2974; SEQ ID NO: 2975; SEQ ID NO: 2976; SEQ ID NO: 2977; SEQ ID NO: 2978; SEQ ID NO: 2979; SEQ ID NO: 2980; SEQ ID NO: 2981; SEQ ID NO: 2982; SEQ ID NO: 2983; SEQ ID NO: 2984; SEQ ID NO: 2985; SEQ ID NO: 2986; SEQ ID NO: 2987; SEQ ID NO: 2988; SEQ ID NO: 2989; SEQ ID NO: 2990; SEQ ID NO: 2991; SEQ ID NO: 2992; SEQ ID NO: 2993; SEQ ID NO: 2994; SEQ ID NO: 2995; SEQ ID NO: 2996; SEQ ID NO: 2997; SEQ ID NO: 2998; SEQ ID NO: 2999; SEQ ID NO: 3000; SEQ ID NO: 3001; SEQ ID NO: 3002; SEQ ID NO: 3003; SEQ ID NO: 3004; SEQ ID NO: 3005; SEQ ID NO: 3006; SEQ ID NO: 3007; SEQ ID NO: 3008; SEQ ID NO: 3009; SEQ ID NO: 3010; SEQ ID NO: 3011; SEQ ID NO: 3012; SEQ ID NO: 3013; SEQ ID NO: 3014; SEQ ID NO: 3015; SEQ ID NO: 3016; SEQ ID NO: 3017; SEQ ID NO: 3018; SEQ ID NO: 3019; SEQ ID NO: 3020; SEQ ID NO: 3021; SEQ ID NO: 3022; SEQ ID NO: 3023; SEQ ID NO: 3024; SEQ ID NO: 3025; SEQ ID NO: 3026; SEQ ID NO: 3027; SEQ ID NO: 3028; SEQ ID NO: 3029; SEQ ID NO: 3030; SEQ ID NO: 3031; SEQ ID NO: 3032; SEQ ID NO: 3033; SEQ ID NO: 3034; SEQ ID NO: 3035; SEQ ID NO: 3036; SEQ ID NO: 3037; SEQ ID NO: 3038; SEQ ID NO: 3039; SEQ ID NO: 3040; SEQ ID NO: 3041; SEQ ID NO: 3042; SEQ ID NO: 3043; SEQ ID NO: 3044; SEQ ID NO: 3045; SEQ ID NO: 3046; SEQ ID NO: 3047; SEQ ID NO: 3048; SEQ ID NO: 3049; SEQ ID NO: 3050; SEQ ID NO: 3051; SEQ ID NO: 3052; SEQ ID NO: 3053; SEQ ID NO: 3054; SEQ ID NO: 3055; SEQ ID NO: 3056; SEQ ID NO: 3057; SEQ ID NO: 3058; SEQ ID NO: 3059; SEQ ID NO: 3060; SEQ ID NO: 3061; SEQ ID NO: 3062; SEQ ID NO: 3063; SEQ ID NO: 3064; SEQ ID NO: 3065; SEQ ID NO: 3066; SEQ ID NO: 3067; SEQ ID NO: 3068; SEQ ID NO: 3069; SEQ ID NO: 3070; SEQ ID NO: 3071; SEQ ID NO: 3072; SEQ ID NO: 3073; SEQ ID NO: 3074; SEQ ID NO: 3075; SEQ ID NO: 3076; SEQ ID NO: 3077; SEQ ID NO: 3078; SEQ ID NO: 3079; SEQ ID NO: 3080; SEQ ID NO: 3081; SEQ ID NO: 3082; SEQ ID NO: 3083; SEQ ID NO: 3084; SEQ ID NO: 3085; SEQ ID NO: 3086; SEQ ID NO: 3087; SEQ ID NO: 3088; SEQ ID NO: 3089; SEQ ID NO: 3090; SEQ ID NO: 3091; SEQ ID NO: 3092; SEQ ID NO: 3093; SEQ ID NO: 3094; SEQ ID NO: 3095; SEQ ID NO: 3096; SEQ ID NO: 3097; SEQ ID NO: 3098; SEQ ID NO: 3099; SEQ ID NO: 3100; SEQ ID NO: 3101; SEQ ID NO: 3102; SEQ ID NO: 3103; SEQ ID NO: 3104; SEQ ID NO: 3105; SEQ ID NO: 3106; SEQ ID NO: 3107; SEQ ID NO: 3108; SEQ ID NO: 3109; SEQ ID NO: 3110; SEQ ID NO: 3111; SEQ ID NO: 3112; SEQ ID NO: 3113; SEQ ID NO: 3114; SEQ ID NO: 3115; SEQ ID NO: 3116; SEQ ID NO: 3117; SEQ ID NO: 3118; SEQ ID NO: 3119; SEQ ID NO: 3120; SEQ ID NO: 3121; SEQ ID NO: 3122; SEQ ID NO: 3123; SEQ ID NO: 3124; SEQ ID NO: 3125; SEQ ID NO: 3126; SEQ ID NO: 3127; SEQ ID NO: 3128; SEQ ID NO: 3129; SEQ ID NO: 3130; SEQ ID NO: 3131; SEQ ID NO: 3132; SEQ ID NO: 3133; SEQ ID NO: 3134; SEQ ID NO: 3135; SEQ ID NO: 3136; SEQ ID NO: 3137; SEQ ID NO:3138; SEQ ID NO: 3139; SEQ ID NO: 3140; SEQ ID NO: 3141; SEQ ID NO: 3142; SEQ ID NO: 3143; SEQ ID NO: 3144; SEQ ID NO: 3145; SEQ ID NO: 3146; SEQ ID NO: 3147; SEQ ID NO: 3148; SEQ ID NO: 3149; SEQ ID NO: 3150; SEQ ID NO: 3151; SEQ ID NO: 3152; SEQ ID NO: 3153; SEQ ID NO: 3154; SEQ ID NO: 3155; SEQ ID NO: 3156; SEQ ID NO: 3157; SEQ ID NO: 3158; SEQ ID NO: 3159; SEQ ID NO: 3160; SEQ ID NO: 3161; SEQ ID NO: 3162; SEQ ID NO: 3163; SEQ ID NO: 3164; SEQ ID NO: 3165; SEQ ID NO: 3166; SEQ ID NO: 3167; SEQ ID NO: 3168; SEQ ID NO: 3169; SEQ ID NO: 3170; SEQ ID NO: 3171; SEQ ID NO: 3172; SEQ ID NO: 3173; SEQ ID NO: 3174; SEQ ID NO: 3175; SEQ ID NO: 3176; SEQ ID NO: 3177; SEQ ID NO: 3178; SEQ ID NO: 3179; SEQ ID NO: 3180; SEQ ID NO: 3181; SEQ ID NO: 3182; SEQ ID NO: 3183; SEQ ID NO: 3184; SEQ ID NO: 3185; SEQ ID NO: 3186; SEQ ID NO: 3187; SEQ ID NO: 3188; SEQ ID NO: 3189; SEQ ID NO: 3190; SEQ ID NO: 3191; SEQ ID NO: 3192; SEQ ID NO: 3193; SEQ ID NO: 3194; SEQ ID NO: 3195; SEQ ID NO: 3196; SEQ ID NO: 3197; SEQ ID NO: 3198; SEQ ID NO: 3199; SEQ ID NO: 3200; SEQ ID NO: 3201; SEQ ID NO: 3202; SEQ ID NO: 3203; SEQ ID NO: 3204; SEQ ID NO: 3205; SEQ ID NO: 3206; SEQ ID NO: 3207; SEQ ID NO: 3208; SEQ ID NO: 3209; SEQ ID NO: 3210; SEQ ID NO: 3211; SEQ ID NO: 3212; SEQ ID NO: 3213; SEQ ID NO: 3214; SEQ ID NO: 3215; SEQ ID NO: 3216; SEQ ID NO: 3217; SEQ ID NO: 3218; SEQ ID NO: 3219; SEQ ID NO: 3220; SEQ ID NO: 3221; SEQ ID NO: 3222; SEQ ID NO: 3223; SEQ ID NO: 3224; SEQ ID NO: 3225; SEQ ID NO: 3226; SEQ ID NO: 3227; SEQ ID NO: 3228; SEQ ID NO: 3229; SEQ ID NO: 3230; SEQ ID NO: 3231; SEQ ID NO: 3232; SEQ ID NO: 3233; SEQ ID NO: 3234; SEQ ID NO: 3235; SEQ ID NO: 3236; SEQ ID NO: 3237; SEQ ID NO: 3238; SEQ ID NO: 3239; SEQ ID NO: 3240; SEQ ID NO: 3241; SEQ ID NO: 3242; SEQ ID NO: 3243; SEQ ID NO: 3244; SEQ ID NO: 3245; SEQ ID NO: 3246; SEQ ID NO: 3247; SEQ ID NO: 3248; SEQ ID NO: 3249; SEQ ID NO: 3250; SEQ ID NO: 3251; SEQ ID NO: 3252; SEQ ID NO: 3253; SEQ ID NO: 3254; SEQ ID NO: 3255; SEQ ID NO: 3256; SEQ ID NO: 3257; SEQ ID NO: 3258; SEQ ID NO: 3259; SEQ ID NO: 3260; SEQ ID NO: 3261; SEQ ID NO: 3262; SEQ ID NO: 3263; SEQ ID NO: 3264; SEQ ID NO: 3265; SEQ ID NO: 3266; SEQ ID NO: 3267; SEQ ID NO: 3268; SEQ ID NO: 3269; SEQ ID NO: 3270; SEQ ID NO: 3271; SEQ ID NO: 3272; SEQ ID NO: 3273; SEQ ID NO: 3274; SEQ ID NO: 3275; SEQ ID NO: 3276; SEQ ID NO: 3277; SEQ ID NO: 3278; SEQ ID NO: 3279; SEQ ID NO: 3280; SEQ ID NO: 3281; SEQ ID NO: 3282; SEQ ID NO: 3283; SEQ ID NO: 3284; SEQ ID NO: 3285; SEQ ID NO: 3286; SEQ ID NO: 3287; SEQ ID NO: 3288; SEQ ID NO: 3289; SEQ ID NO: 3290; SEQ ID NO: 3291; SEQ ID NO: 3292; SEQ ID NO: 3293; SEQ ID NO: 3294; SEQ ID NO: 3295; SEQ ID NO: 3296; SEQ ID NO: 3297; SEQ ID NO: 3298; SEQ ID NO: 3299; SEQ ID NO: 3300; SEQ ID NO: 3301; SEQ ID NO: 3302; SEQ ID NO: 3303; SEQ ID NO: 3304; SEQ ID NO: 3305; SEQ ID NO: 3306; SEQ ID NO: 3307; SEQ ID NO: 3308; SEQ ID NO: 3309; SEQ ID NO: 3310; SEQ ID NO: 3311; SEQ ID NO: 3312; SEQ ID NO: 3313; SEQ ID NO: 3314; SEQ ID NO: 3315; SEQ ID NO: 3316; SEQ ID NO: 3317; SEQ ID NO: 3318; SEQ ID NO: 3319; SEQ ID NO: 3320; SEQ ID NO: 3321; SEQ ID NO: 3322; SEQ ID NO: 3323; SEQ ID NO: 3324; SEQ ID NO: 3325; SEQ ID NO: 3326; SEQ ID NO: 3327; SEQ ID NO: 3328; SEQ ID NO: 3329; SEQ ID NO: 3330; SEQ ID NO: 3331; SEQ ID NO: 3332; SEQ ID NO: 3333; SEQ ID NO: 3334; SEQ ID NO: 3335; SEQ ID NO: 3336; SEQ ID NO: 3337; SEQ ID NO: 3338; SEQ ID NO: 3339; SEQ ID NO: 3340; SEQ ID NO: 3341; SEQ ID NO: 3342; SEQ ID NO: 3343; SEQ ID NO: 3344; SEQ ID NO: 3345; SEQ ID NO: 3346; SEQ ID NO: 3347; SEQ ID NO: 3348; SEQ ID NO: 3349; SEQ ID NO: 3350; SEQ ID NO: 3351; SEQ ID NO: 3352; SEQ ID NO: 3353; SEQ ID NO: 3354; SEQ ID NO: 3355; SEQ ID NO: 3356; SEQ ID NO: 3357; SEQ ID NO: 3358; SEQ ID NO: 3359; SEQ ID NO: 3360; SEQ ID NO: 3361; SEQ ID NO: 3362; SEQ ID NO: 3363; SEQ ID NO: 3364; SEQ ID NO: 3365; SEQ ID NO: 3366; SEQ ID NO: 3367; SEQ ID NO: 3368; SEQ ID NO: 3369; SEQ ID NO: 3370; SEQ ID NO: 3371; SEQ ID NO: 3372; SEQ ID NO: 3373; SEQ ID NO: 3374; SEQ ID NO: 3375; SEQ ID NO: 3376; SEQ ID NO: 3377; SEQ ID NO: 3378; SEQ ID NO: 3379; SEQ ID NO: 3380; SEQ ID NO: 3381; SEQ ID NO: 3382; SEQ ID NO: 3383; SEQ ID NO: 3384; SEQ ID NO: 3385; SEQ ID NO: 3386; SEQ ID NO: 3387; SEQ ID NO: 3388; SEQ ID NO: 3389; SEQ ID NO: 3390; SEQ ID NO: 3391; SEQ ID NO: 3392; SEQ ID NO: 3393; SEQ ID NO: 3394; SEQ ID NO: 3395; SEQ ID NO: 3396; SEQ ID NO: 3397; SEQ ID NO: 3398; SEQ ID NO: 3399; SEQ ID NO: 3400; SEQ ID NO: 3401; SEQ ID NO: 3402; SEQ ID NO: 3403; SEQ ID NO: 3404; SEQ ID NO: 3405; SEQ ID NO: 3406; SEQ ID NO: 3407; SEQ ID NO: 3408; SEQ ID NO: 3409; SEQ ID NO: 3410; SEQ ID NO: 3411; SEQ ID NO: 3412; SEQ ID NO: 3413; SEQ ID NO: 3414; SEQ ID NO: 3415; SEQ ID NO: 3416; SEQ ID NO: 3417; SEQ ID NO: 3418; SEQ ID NO: 3419; SEQ ID NO: 3420; SEQ ID NO: 3421; SEQ ID NO: 3422; SEQ ID NO: 3423; SEQ ID NO: 3424; SEQ ID NO: 3425; SEQ ID NO: 3426; SEQ ID NO: 3427; SEQ ID NO: 3428; SEQ ID NO: 3429; SEQ ID NO: 3430; SEQ ID NO: 3431; SEQ ID NO: 3432; SEQ ID NO: 3433; SEQ ID NO: 3434; SEQ ID NO: 3435; SEQ ID NO: 3436; SEQ ID NO: 3437; SEQ ID NO: 3438; SEQ ID NO: 3439; SEQ ID NO: 3440; SEQ ID NO: 3441; SEQ ID NO: 3442; SEQ ID NO: 3443; SEQ ID NO: 3444; SEQ ID NO: 3445; SEQ ID NO: 3446; SEQ ID NO: 3447; SEQ ID NO: 3448; SEQ ID NO: 3449; SEQ ID NO: 3450; SEQ ID NO: 3451; SEQ ID NO: 3452; SEQ ID NO: 3453; SEQ ID NO: 3454; SEQ ID NO: 3455; SEQ ID NO: 3456; SEQ ID NO: 3457; SEQ ID NO: 3458; SEQ ID NO: 3459, SEQ ID NO: 3460; SEQ ID NO: 3461; SEQ ID NO: 3462; SEQ ID NO: 3463; SEQ ID NO: 3464; SEQ ID NO: 3465; SEQ ID NO: 3466; SEQ ID NO: 3467; SEQ ID NO: 3468; SEQ ID NO: 3469; SEQ ID NO: 3470; SEQ ID NO: 3471; SEQ ID NO: 3472; SEQ ID NO: 3473; SEQ ID NO: 3474; SEQ ID NO: 3475; SEQ ID NO: 3476; SEQ ID NO: 3477; SEQ ID NO: 3478; SEQ ID NO: 3479; SEQ ID NO: 3480; SEQ ID NO: 3481; SEQ ID NO: 3482; SEQ ID NO: 3483; SEQ ID NO: 3484; SEQ ID NO: 3485; SEQ ID NO: 3486; SEQ ID NO: 3487; SEQ ID NO: 3488; SEQ ID NO: 3489; SEQ ID NO: 3490; SEQ ID NO: 3491; SEQ ID NO: 3492; SEQ ID NO: 3493; SEQ ID NO: 3494; SEQ ID NO: 3495; SEQ ID NO: 3496; SEQ ID NO: 3497; SEQ ID NO: 3498; SEQ ID NO: 3499; SEQ ID NO: 3500; SEQ ID NO: 3501; SEQ ID NO: 3502; SEQ ID NO: 3503; SEQ ID NO: 3504; SEQ ID NO: 3505; SEQ ID NO: 3506; SEQ ID NO: 3507; SEQ ID NO: 3508; SEQ ID NO: 3509; SEQ ID NO: 3510; SEQ ID NO: 3511; SEQ ID NO: 3512; SEQ ID NO: 3513; SEQ ID NO: 3514; SEQ ID NO: 3515; SEQ ID NO: 3516; SEQ ID NO: 3517; SEQ ID NO: 3518; SEQ ID NO: 3519; SEQ ID NO: 3520; SEQ ID NO: 3521; SEQ ID NO: 3522; SEQ ID NO: 3523; SEQ ID NO: 3524; SEQ ID NO: 3525; SEQ ID NO: 3526; SEQ ID NO: 3527; SEQ ID NO: 3528; SEQ ID NO: 3529; SEQ ID NO: 3530; SEQ ID NO: 3531; SEQ ID NO: 3532; SEQ ID NO: 3533; SEQ ID NO: 3534; SEQ ID NO: 3535; SEQ ID NO: 3536; SEQ ID NO: 3537; SEQ ID NO: 3538; SEQ ID NO: 3539; SEQ ID NO: 3540; SEQ ID NO: 3541; SEQ ID NO: 3542; SEQ ID NO: 3543; SEQ ID NO: 3544; SEQ ID NO: 3545; SEQ ID NO: 3546; SEQ ID NO: 3547; SEQ ID NO: 3548; SEQ ID NO: 3549; SEQ ID NO: 3550; SEQ ID NO: 3551; SEQ ID NO: 3552; SEQ ID NO: 3553; SEQ ID NO: 3554; SEQ ID NO: 3555; SEQ ID NO: 3556; SEQ ID NO: 3557; SEQ ID NO: 3558; SEQ ID NO: 3559; SEQ ID NO: 3560; SEQ ID NO: 3561; SEQ ID NO: 3562; SEQ ID NO: 3563; SEQ ID NO: 3564; SEQ ID NO: 3565; SEQ ID NO: 3566; SEQ ID NO: 3567; SEQ ID NO: 3568; SEQ ID NO: 3569; SEQ ID NO: 3570; SEQ ID NO: 3571; SEQ ID NO: 3572; SEQ ID NO: 3573; SEQ ID NO: 3574; SEQ ID NO: 3575; SEQ ID NO: 3576; SEQ ID NO: 3577; SEQ ID NO: 3578; SEQ ID NO: 3579; SEQ ID NO: 3580; SEQ ID NO: 3581; SEQ ID NO: 3582; SEQ ID NO: 3583; SEQ ID NO: 3584; SEQ ID NO: 3585; SEQ ID NO: 3586; SEQ ID NO: 3587; SEQ ID NO: 3588; SEQ ID NO: 3589; SEQ ID NO: 3590; SEQ ID NO: 3591; SEQ ID NO: 3592; SEQ ID NO: 3593; SEQ ID NO: 3594; SEQ ID NO: 3595; SEQ ID NO: 3596; SEQ ID NO: 3597; SEQ ID NO: 3598; SEQ ID NO: 3599; SEQ ID NO: 3600; SEQ ID NO: 3601; SEQ ID NO: 3602; SEQ ID NO: 3603; SEQ ID NO: 3604; SEQ ID NO: 3605; SEQ ID NO: 3606; SEQ ID NO: 3607; SEQ ID NO: 3608; SEQ ID NO: 3609; SEQ ID NO: 3610; SEQ ID NO: 3611; SEQ ID NO: 3612; SEQ ID NO: 3613; SEQ ID NO: 3614; SEQ ID NO: 3615; SEQ ID NO: 3616; SEQ ID NO: 3617; SEQ ID NO: 3618; SEQ ID NO: 3619; SEQ ID NO: 3620; SEQ ID NO: 3621; SEQ ID NO: 3622; SEQ ID NO: 3623; SEQ ID NO: 3624; SEQ ID NO: 3625; SEQ ID NO: 3626; SEQ ID NO: 3627; SEQ ID NO: 3628; SEQ ID NO: 3629; SEQ ID NO: 3630; SEQ ID NO: 3631; SEQ ID NO: 3632; SEQ ID NO: 3633; SEQ ID NO: 3634; SEQ ID NO: 3635; SEQ ID NO: 3636; SEQ ID NO: 3637; SEQ ID NO: 3638; SEQ ID NO: 3639; SEQ ID NO: 3640; SEQ ID NO: 3641; SEQ ID NO: 3642; SEQ ID NO: 3643; SEQ ID NO: 3644; SEQ ID NO: 3645; SEQ ID NO: 3646; SEQ ID NO: 3647; SEQ ID NO: 3648; SEQ ID NO: 3649; SEQ ID NO: 3650; SEQ ID NO: 3651; SEQ ID NO: 3652; SEQ ID NO: 3653; SEQ ID NO: 3654; SEQ ID NO: 3655; SEQ ID NO: 3656; SEQ ID NO: 3657; SEQ ID NO: 3658; SEQ ID NO: 3659; SEQ ID NO: 3660; SEQ ID NO: 3661; SEQ ID NO: 3662; SEQ ID NO: 3663; SEQ ID NO: 3664; SEQ ID NO: 3665; SEQ ID NO: 3666; SEQ ID NO: 3667; SEQ ID NO: 3668; SEQ ID NO: 3669; SEQ ID NO: 3670; SEQ ID NO: 3671; SEQ ID NO: 3672; SEQ ID NO: 3673; SEQ ID NO: 3674; SEQ ID NO: 3675; SEQ ID NO: 3676; SEQ ID NO: 3677; SEQ ID NO: 3678; SEQ ID NO: 3679; SEQ ID NO: 3680; SEQ ID NO: 3681; SEQ ID NO: 3682; SEQ ID NO: 3683; SEQ ID NO: 3684; SEQ ID NO: 3685; SEQ ID NO: 3686; SEQ ID NO: 3687; SEQ ID NO: 3688; SEQ ID NO: 3689; SEQ ID NO: 3690; SEQ ID NO: 3691; SEQ ID NO: 3692; SEQ ID NO: 3693; SEQ ID NO: 3694; SEQ ID NO: 3695; SEQ ID NO: 3696; SEQ ID NO: 3697; SEQ ID NO: 3698; SEQ ID NO: 3699; SEQ ID NO: 3700; SEQ ID NO: 3701; SEQ ID NO: 3702; SEQ ID NO: 3703; SEQ ID NO: 3704; SEQ ID NO: 3705; SEQ ID NO: 3706; SEQ ID NO: 3707; SEQ ID NO: 3708; SEQ ID NO: 3709; SEQ ID NO: 3710; SEQ ID NO: 3711; SEQ ID NO: 3712; SEQ ID NO: 3713; SEQ ID NO: 3714; SEQ ID NO: 3715; SEQ ID NO: 3716; SEQ ID NO: 3717; SEQ ID NO: 3718; SEQ ID NO: 3719; SEQ ID NO: 3720; SEQ ID NO: 3721; SEQ ID NO: 3722; SEQ ID NO: 3723; SEQ ID NO: 3724; SEQ ID NO: 3725; SEQ ID NO: 3726; SEQ ID NO: 3727; SEQ ID NO: 3728; SEQ ID NO: 3729; SEQ ID NO: 3730; SEQ ID NO: 3731; SEQ ID NO: 3732; SEQ ID NO: 3733; SEQ ID NO: 3734; SEQ ID NO: 3735; SEQ ID NO: 3736; SEQ ID NO: 3737; SEQ ID NO: 3738; SEQ ID NO: 3739; SEQ ID NO: 3740; SEQ ID NO: 3741; SEQ ID NO: 3742; SEQ ID NO: 3743; SEQ ID NO: 3744; SEQ ID NO: 3745; SEQ ID NO: 3746; SEQ ID NO: 3747; SEQ ID NO: 3748; SEQ ID NO: 3749; SEQ ID NO: 3750; SEQ ID NO: 3751; SEQ ID NO: 3752; SEQ ID NO: 3753; SEQ ID NO: 3754; SEQ ID NO: 3755; SEQ ID NO: 3756; SEQ ID NO: 3757; SEQ ID NO: 3758; SEQ ID NO: 3759; SEQ ID NO: 3760; SEQ ID NO: 3761; SEQ ID NO: 3762; SEQ ID NO: 3763; SEQ ID NO: 3764; SEQ ID NO: 3765; SEQ ID NO: 3766; SEQ ID NO: 3767; SEQ ID NO: 3768; SEQ ID NO: 3769; SEQ ID NO: 3770; SEQ ID NO: 3771; SEQ ID NO: 3772; SEQ ID NO: 3773; SEQ ID NO: 3774; SEQ ID NO: 3775; SEQ ID NO: 3776; SEQ ID NO: 3777; SEQ ID NO: 3778; SEQ ID NO: 3779; SEQ ID NO: 3780; SEQ ID NO: 3781; SEQ ID NO: 3782; SEQ ID NO: 3783; SEQ ID NO: 3784; SEQ ID NO: 3785; SEQ ID NO: 3786; SEQ ID NO: 3787; SEQ ID NO: 3788; SEQ ID NO: 3789; SEQ ID NO: 3790; SEQ ID NO: 3791; SEQ ID NO: 3792; SEQ ID NO: 3793; SEQ ID NO: 3794; SEQ ID NO: 3795; SEQ ID NO: 3796; SEQ ID NO: 3797; SEQ ID NO: 3798; SEQ ID NO: 3799; SEQ ID NO: 3800; SEQ ID NO: 3801; SEQ ID NO: 3802; SEQ ID NO: 3803; SEQ ID NO: 3804; SEQ ID NO: 3805; SEQ ID NO: 3806; SEQ ID NO: 3807; SEQ ID NO: 3808; SEQ ID NO: 3809; SEQ ID NO: 3810; SEQ ID NO: 3811; SEQ ID NO: 3812; SEQ ID NO: 3813; SEQ ID NO: 3814; SEQ ID NO: 3815; SEQ ID NO: 3816; SEQ ID NO: 3817; SEQ ID NO: 3818; SEQ ID NO: 3819; SEQ ID NO: 3820; SEQ ID NO: 3821; SEQ ID NO: 3822; SEQ ID NO: 3823; SEQ ID NO: 3824; SEQ ID NO: 3825; SEQ ID NO: 3826; SEQ ID NO: 3827; SEQ ID NO: 3828; SEQ ID NO: 3829; SEQ ID NO: 3830; SEQ ID NO: 3831; SEQ ID NO: 3832; SEQ ID NO: 3833; SEQ ID NO: 3834; SEQ ID NO: 3835; SEQ ID NO: 3836; SEQ ID NO: 3837; SEQ ID NO: 3838; SEQ ID NO: 3839; SEQ ID NO: 3840; SEQ ID NO: 3841; SEQ ID NO: 3842; SEQ ID NO: 3843; SEQ ID NO: 3844; SEQ ID NO: 3845; SEQ ID NO: 3846; SEQ ID NO: 3847; SEQ ID NO: 3848; SEQ ID NO: 3849; SEQ ID NO: 3850; SEQ ID NO: 3851; SEQ ID NO: 3852; SEQ ID NO: 3853; SEQ ID NO: 3854; SEQ ID NO: 3855; SEQ ID NO: 3856; SEQ ID NO: 3857; SEQ ID NO: 3858; SEQ ID NO: 3859; SEQ ID NO: 3860; SEQ ID NO: 3861; SEQ ID NO: 3862; SEQ ID NO: 3863; SEQ ID NO: 3864; SEQ ID NO: 3865; SEQ ID NO: 3866; SEQ ID NO: 3867; SEQ ID NO: 3868; SEQ ID NO: 3869; SEQ ID NO: 3870; SEQ ID NO: 3871; SEQ ID NO: 3872; SEQ ID NO: 3873; SEQ ID NO: 3874; SEQ ID NO: 3875; SEQ ID NO: 3876; SEQ ID NO: 3877; SEQ ID NO: 3878; SEQ ID NO: 3879; SEQ ID NO: 3880; SEQ ID NO: 3881; SEQ ID NO: 3882; SEQ ID NO: 3883; SEQ ID NO: 3884; SEQ ID NO: 3885; SEQ ID NO: 3886; SEQ ID NO: 3887; SEQ ID NO: 3888; SEQ ID NO: 3889; SEQ ID NO: 3890; SEQ ID NO: 3891; SEQ ID NO: 3892; SEQ ID NO: 3893; SEQ ID NO: 3894; SEQ ID NO: 3895; SEQ ID NO: 3896; SEQ ID NO: 3897; SEQ ID NO: 3898; SEQ ID NO: 3899; SEQ ID NO: 3900; SEQ ID NO: 3901; SEQ ID NO: 3902; SEQ ID NO: 3903; SEQ ID NO: 3904; SEQ ID NO: 3905; SEQ ID NO: 3906; SEQ ID NO: 3907; SEQ ID NO: 3908; SEQ ID NO: 3909; SEQ ID NO: 3910; SEQ ID NO: 3911; SEQ ID NO: 3912; SEQ ID NO: 3913; SEQ ID NO: 3914; SEQ ID NO: 3915; SEQ ID NO: 3916; SEQ ID NO: 3917; SEQ ID NO: 3918; SEQ ID NO: 3919; SEQ ID NO: 3920; SEQ ID NO: 3921; SEQ ID NO: 3922; SEQ ID NO: 3923; SEQ ID NO: 3924; SEQ ID NO: 3925; SEQ ID NO: 3926; SEQ ID NO: 3927; SEQ ID NO: 3928; SEQ ID NO: 3929; SEQ ID NO: 3930; SEQ ID NO: 3931; SEQ ID NO: 3932; SEQ ID NO: 3933; SEQ ID NO: 3934; SEQ ID NO: 3935; SEQ ID NO: 3936; SEQ ID NO: 3937; SEQ ID NO: 3938; SEQ ID NO: 3939; SEQ ID NO: 3940; SEQ ID NO: 3941; SEQ ID NO: 3942; SEQ ID NO: 3943; SEQ ID NO: 3944; SEQ ID NO: 3945; SEQ ID NO: 3946; SEQ ID NO: 3947; SEQ ID NO: 3948; SEQ ID NO: 3949; SEQ ID NO: 3950; SEQ ID NO: 3951; SEQ ID NO: 3952; SEQ ID NO: 3953; SEQ ID NO: 3954; SEQ ID NO: 3955; SEQ ID NO: 3956; SEQ ID NO: 3957; SEQ ID NO: 3958; SEQ ID NO: 3959; SEQ ID NO: 3960; SEQ ID NO: 3961; SEQ ID NO: 3962; SEQ ID NO: 3963; SEQ ID NO: 3964; SEQ ID NO: 3965; SEQ ID NO: 3966; SEQ ID NO: 3967; SEQ ID NO: 3968; SEQ ID NO: 3969; SEQ ID NO: 3970; SEQ ID NO: 3971; SEQ ID NO: 3972; SEQ ID NO: 3973; SEQ ID NO: 3974; SEQ ID NO: 3975; SEQ ID NO: 3976; SEQ ID NO: 3977; SEQ ID NO: 3978; SEQ ID NO: 3979; SEQ ID NO: 3980; SEQ ID NO: 3981; SEQ ID NO: 3982; SEQ ID NO: 3983; SEQ ID NO: 3984; SEQ ID NO: 3985; SEQ ID NO: 3986; SEQ ID NO: 3987; SEQ ID NO: 3988; SEQ ID NO: 3989; SEQ ID NO: 3990; SEQ ID NO: 3991; SEQ ID NO: 3992; SEQ ID NO: 3993; SEQ ID NO: 3994; SEQ ID NO: 3995; SEQ ID NO: 3996; SEQ ID NO: 3997; SEQ ID NO: 3998; SEQ ID NO: 3999; SEQ ID NO: 4000; SEQ ID NO: 4001; SEQ ID NO: 4002; SEQ ID NO: 4003; SEQ ID NO: 4004; SEQ ID NO: 4005; SEQ ID NO: 4006; SEQ ID NO: 4007; SEQ ID NO: 4008; SEQ ID NO: 4009; SEQ ID NO: 4010; SEQ ID NO: 4011; SEQ ID NO: 4012; SEQ ID NO: 4013; SEQ ID NO: 4014; SEQ ID NO: 4015; and SEQ ID NO: 9593; SEQ ID NO: 9594; SEQ ID NO: 9595; SEQ ID NO: 9596; SEQ ID NO: 9597; SEQ ID NO: 9598; SEQ ID NO: 9599; SEQ ID NO: 9600; SEQ ID NO: 9601; SEQ ID NO: 9602; SEQ ID NO: 9603; SEQ ID NO: 9604; SEQ ID NO: 9605; SEQ ID NO: 9606; SEQ ID NO: 9607; SEQ ID NO: 9608; SEQ ID NO: 9609; SEQ ID NO: 9610; SEQ ID NO: 9611; SEQ ID NO: 9612; SEQ ID NO: 9613; SEQ ID NO: 9614; SEQ ID NO: 9615; SEQ ID NO: 9616; SEQ ID NO: 9617; SEQ ID NO: 9618; SEQ ID NO: 9619; SEQ ID NO: 9620; SEQ ID NO: 9621, or a complement thereof.  
     [0075] In one embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in energy metabolism encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1576; SEQ ID NO: 1577; SEQ ID NO: 1578; SEQ ID NO: 1579; SEQ ID NO: 1580; SEQ ID NO: 1581; SEQ ID NO: 1582; SEQ ID NO: 1583; SEQ ID NO: 1584; SEQ ID NO: 1585; SEQ ID NO: 1586; SEQ ID NO: 1587; SEQ ID NO: 1588; SEQ ID NO: 1589; SEQ ID NO: 1590; SEQ ID NO: 1591; SEQ ID NO: 1592; SEQ ID NO: 1593; SEQ ID NO: 1594; SEQ ID NO: 1595; SEQ ID NO: 1596; SEQ ID NO: 1597; SEQ ID NO: 1598; SEQ ID NO: 1599; SEQ ID NO: 1600; SEQ ID NO: 1601; SEQ ID NO: 1602; SEQ ID NO: 1603; SEQ ID NO: 1604; SEQ ID NO: 1605; SEQ ID NO: 1606; SEQ ID NO: 1607; SEQ ID NO: 1608; SEQ ID NO: 1609; SEQ ID NO: 1610; SEQ ID NO: 1611; SEQ ID NO: 1612; SEQ ID NO: 1613; SEQ ID NO: 1614; SEQ ID NO: 1615; SEQ ID NO: 1616; SEQ ID NO: 1617; SEQ ID NO: 1618; SEQ ID NO: 1619; SEQ ID NO: 1620; SEQ ID NO: 1621; SEQ ID NO: 1622; SEQ ID NO: 1623; SEQ ID NO: 1624; SEQ ID NO: 1625; SEQ ID NO: 1626; SEQ ID NO: 1627; SEQ ID NO: 1628; SEQ ID NO: 1629; SEQ ID NO: 1630; SEQ ID NO: 1631; SEQ ID NO: 1632; SEQ ID NO: 1633; SEQ ID NO: 1634; SEQ ID NO: 1635; SEQ ID NO: 1636; SEQ ID NO: 1637; SEQ ID NO: 1638; SEQ ID NO: 1639; SEQ ID NO: 1640; SEQ ID NO: 1641; SEQ ID NO: 1642; SEQ ID NO: 1643; SEQ ID NO: 1644; SEQ ID NO: 1645; SEQ ID NO: 1646; SEQ ID NO: 1647; SEQ ID NO: 1648; SEQ ID NO: 1649; SEQ ID NO: 1650; SEQ ID NO: 1651; SEQ ID NO: 1652; SEQ ID NO: 1653; SEQ ID NO: 1654; SEQ ID NO: 1655; SEQ ID NO: 1656; SEQ ID NO: 1657; SEQ ID NO: 1658; SEQ ID NO: 1659; SEQ ID NO: 1660; SEQ ID NO: 1661; SEQ ID NO: 1662; SEQ ID NO: 1663; SEQ ID NO: 1664; SEQ ID NO: 1665; SEQ ID NO: 1666; SEQ ID NO: 1667; SEQ ID NO: 1668; SEQ ID NO: 1669; SEQ ID NO: 1670; SEQ ID NO: 1671; SEQ ID NO: 1672; SEQ ID NO: 1673; SEQ ID NO: 1674; SEQ ID NO: 1675; SEQ ID NO: 1676; SEQ ID NO: 1677; SEQ ID NO: 1678; SEQ ID NO: 1679; SEQ ID NO: 1680; SEQ ID NO: 1681; SEQ ID NO: 1682; SEQ ID NO: 1683; SEQ ID NO: 1684; SEQ ID NO: 1685; SEQ ID NO: 1686; SEQ ID NO: 1687; SEQ ID NO: 1688; SEQ ID NO: 1689; SEQ ID NO: 1690; SEQ ID NO: 1691; SEQ ID NO: 1692; SEQ ID NO: 1693; SEQ ID NO: 1694; SEQ ID NO: 1695; SEQ ID NO: 1696; SEQ ID NO: 1697; SEQ ID NO: 1698; SEQ ID NO: 1699; SEQ ID NO: 1700; SEQ ID NO: 1701; SEQ ID NO: 1702; SEQ ID NO: 1703; SEQ ID NO: 1704; SEQ ID NO: 1705; SEQ ID NO: 1706; SEQ ID NO: 1707; SEQ ID NO: 1708; SEQ ID NO: 1709; SEQ ID NO: 1710; SEQ ID NO: 1711; SEQ ID NO: 1712; SEQ ID NO: 1713; SEQ ID NO: 1714; SEQ ID NO: 1715; SEQ ID NO: 1716; SEQ ID NO: 1717; SEQ ID NO: 1718; SEQ ID NO: 1719; SEQ ID NO: 1720; SEQ ID NO: 1721; SEQ ID NO: 1722; SEQ ID NO: 1723; SEQ ID NO: 1724; SEQ ID NO: 1725; and SEQ ID NO: 9593; SEQ ID NO: 9594, or a complement thereof.  
     [0076] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in amino acid metabolism encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1726; SEQ ID NO: 1727; SEQ ID NO: 1728; SEQ ID NO: 1729; SEQ ID NO: 1730; SEQ ID NO: 1731; SEQ ID NO: 1732; SEQ ID NO: 1733; SEQ ID NO: 1734; SEQ ID NO: 1735; SEQ ID NO: 1736; SEQ ID NO: 1737; SEQ ID NO: 1738; SEQ ID NO: 1739; SEQ ID NO: 1740; SEQ ID NO: 1741; SEQ ID NO: 1742; SEQ ID NO: 1743; SEQ ID NO: 1744; SEQ ID NO: 1745; SEQ ID NO: 1746; SEQ ID NO: 1747; SEQ ID NO: 1748; SEQ ID NO: 1749; SEQ ID NO: 1750; SEQ ID NO: 1751; SEQ ID NO: 1752; SEQ ID NO: 1753; SEQ ID NO: 1754; SEQ ID NO: 1755; SEQ ID NO: 1756; SEQ ID NO: 1757; SEQ ID NO: 1758; SEQ ID NO: 1759; SEQ ID NO: 1760; SEQ ID NO: 1761; SEQ ID NO: 1762; SEQ ID NO: 1763; SEQ ID NO: 1764; SEQ ID NO: 1765; SEQ ID NO: 1766; SEQ ID NO: 1767; SEQ ID NO: 1768; SEQ ID NO: 1769; SEQ ID NO: 1770; SEQ ID NO: 1771; SEQ ID NO: 1772; SEQ ID NO: 1773; SEQ ID NO: 1774; SEQ ID NO: 1775; SEQ ID NO: 1776; SEQ ID NO: 1777; SEQ ID NO: 1778; SEQ ID NO: 1779; SEQ ID NO: 1780; SEQ ID NO: 1781; SEQ ID NO: 1782; SEQ ID NO: 1783; SEQ ID NO: 1784; SEQ ID NO: 1785; SEQ ID NO: 1786; SEQ ID NO: 1787; SEQ ID NO: 1788; SEQ ID NO: 1789; SEQ ID NO: 1790; SEQ ID NO: 1791; SEQ ID NO: 1792; SEQ ID NO: 1793; SEQ ID NO: 1794; SEQ ID NO: 1795; SEQ ID NO: 1796; SEQ ID NO: 1797; SEQ ID NO: 1798; SEQ ID NO: 1799; SEQ ID NO: 1800; SEQ ID NO: 1801; SEQ ID NO: 1802; SEQ ID NO: 1803; SEQ ID NO: 1804; SEQ ID NO: 1805; SEQ ID NO: 1806; SEQ ID NO: 1807; SEQ ID NO: 1808; SEQ ID NO: 1809; SEQ ID NO: 1810; SEQ ID NO: 1811; SEQ ID NO: 1812; SEQ ID NO: 1813; SEQ ID NO: 1814; SEQ ID NO: 1815; SEQ ID NO: 1816; SEQ ID NO: 1817; SEQ ID NO: 1818; SEQ ID NO: 1819; SEQ ID NO: 1820; SEQ ID NO: 1821; SEQ ID NO: 1822; SEQ ID NO: 1823; SEQ ID NO: 1824; SEQ ID NO: 1825; SEQ ID NO: 1826; SEQ ID NO: 1827; SEQ ID NO: 1828; SEQ ID NO: 1829; SEQ ID NO: 1830; SEQ ID NO: 1831; SEQ ID NO: 1832; SEQ ID NO: 1833; SEQ ID NO: 1834; SEQ ID NO: 1835; SEQ ID NO: 1836; SEQ ID NO: 1837; SEQ ID NO: 1838; SEQ ID NO: 1839; SEQ ID NO: 1840; SEQ ID NO: 1841; SEQ ID NO: 1842; SEQ ID NO: 1843; SEQ ID NO: 1844; SEQ ID NO: 1845; SEQ ID NO: 1846; SEQ ID NO: 1847; SEQ ID NO: 1848; SEQ ID NO: 1849; SEQ ID NO: 1850; SEQ ID NO: 1851; SEQ ID NO: 1852; SEQ ID NO: 1853; SEQ ID NO: 1854; SEQ ID NO: 1855; SEQ ID NO: 1856; SEQ ID NO: 1857; SEQ ID NO: 1858; SEQ ID NO: 1859; SEQ ID NO: 1860; SEQ ID NO: 1861; SEQ ID NO: 1862; SEQ ID NO: 1863; SEQ ID NO: 1864; SEQ ID NO: 1865; SEQ ID NO: 1866; SEQ ID NO: 1867; SEQ ID NO: 1868 and SEQ ID NO: 9595; SEQ ID NO: 9596, or a complement thereof.  
     [0077] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in cofactor metabolism encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1869; SEQ ID NO: 1870; SEQ ID NO: 1871; SEQ ID NO: 1872; SEQ ID NO: 1873; SEQ ID NO: 1874; SEQ ID NO: 1875; SEQ ID NO: 1876; SEQ ID NO: 1877; SEQ ID NO: 1878; SEQ ID NO: 1879; SEQ ID NO: 1880; SEQ ID NO: 1881; SEQ ID NO: 1882; SEQ ID NO: 1883; SEQ ID NO: 1884; SEQ ID NO: 1885; SEQ ID NO: 1886; SEQ ID NO: 1887; SEQ ID NO: 1888; SEQ ID NO: 1889; SEQ ID NO: 1890; SEQ ID NO: 1891; SEQ ID NO: 1892; SEQ ID NO: 1893; SEQ ID NO: 1894; SEQ ID NO: 1895; SEQ ID NO: 1896; SEQ ID NO: 1897; SEQ ID NO: 1898; SEQ ID NO: 1899; SEQ ID NO: 1900; SEQ ID NO: 1901; SEQ ID NO: 1902; SEQ ID NO: 1903; SEQ ID NO: 1904; SEQ ID NO: 1905;-SEQ ID NO: 1906; SEQ ID NO: 1907; SEQ ID NO: 1908; SEQ ID NO: 1909; SEQ ID NO: 1910; SEQ ID NO: 1911; SEQ ID NO: 1912; SEQ ID NO: 1913; SEQ ID NO: 1914; SEQ ID NO: 1915; SEQ ID NO: 1916; SEQ ID NO: 1917; SEQ ID NO: 1918; SEQ ID NO: 1919; SEQ ID NO: 1920; SEQ ID NO: 1921; SEQ ID NO: 1922; SEQ ID NO: 1923; SEQ ID NO: 1924; SEQ ID NO: 1925; SEQ ID NO: 1926; SEQ ID NC. 927; SEQ ID NO: 1928; SEQ ID NO: 1929; SEQ ID NO: 1930; SEQ ID NO: 1931; SEQ ID NO: 1932; SEQ ID NO: 1933; SEQ ID NO: 1934; SEQ ID NO: 1935; SEQ ID NO: 1936; SEQ ID NO: 1937; SEQ ID NO: 1938; SEQ ID NO: 1939; SEQ ID NO: 1940; SEQ ID NO: 1941; SEQ ID NO: 1942; SEQ ID NO: 1943; SEQ ID NO: 1944; SEQ ID NO: 1945; SEQ ID NO: 1946; SEQ ID NO: 1947; SEQ ID NO: 1948; SEQ ID NO: 1949; SEQ ID NO: 1950; SEQ ID NO: 1951; SEQ ID NO: 1952; SEQ ID NO: 1953; SEQ ID NO: 1954; SEQ ID NO: 1955; SEQ ID NO: 1956; SEQ ID NO: 1957; SEQ ID NO: 1958; SEQ ID NO: 1959; SEQ ID NO: 1960; SEQ ID NO: 1961; SEQ ID NO: 1962; SEQ ID NO: 1963; SEQ ID NO: 1964; SEQ ID NO: 1965; SEQ ID NO: 1966; SEQ ID NO: 1967; SEQ ID NO: 1968; SEQ ID NO: 1969; SEQ ID NO: 1970; SEQ ID NO: 1971; SEQ ID NO: 1972; SEQ ID NO: 1973; SEQ ID NO: 1974; SEQ ID NO: 1975; SEQ ID NO: 1976; SEQ ID NO: 1977; SEQ ID NO: 1978; SEQ ID NO: 1979; SEQ ID NO: 1980; SEQ ID NO: 1981; SEQ ID NO: 1982; SEQ ID NO: 1983; SEQ ID NO: 1984; SEQ ID NO: 1985; SEQ ID NO: 1986; SEQ ID NO: 1987; SEQ ID NO: 1988; SEQ ID NO: 1989; SEQ ID NO: 1990; SEQ ID NO: 1991; SEQ ID NO: 1992; SEQ ID NO: 1993; SEQ ID NO: 1994; SEQ ID NO: 1995; SEQ ID NO: 1996; SEQ ID NO: 1997; SEQ ID NO: 1998; SEQ ID NO: 1999; SEQ ID NO: 2000; SEQ ID NO: 2001; SEQ ID NO: 2002; SEQ ID NO: 2003; SEQ ID NO: 2004; SEQ ID NO: 2005; and SEQ ID NO: 9597, or a complement thereof.  
     [0078] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in carbohydrate metabolism encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2006; SEQ ID NO: 2007; SEQ ID NO: 2008; SEQ ID NO: 2009; SEQ ID NO: 2010; SEQ ID NO: 2011; SEQ ID NO: 2012; SEQ ID NO: 2013; SEQ ID NO: 2014; SEQ ID NO: 2015; SEQ ID NO: 2016; SEQ ID NO: 2017; SEQ ID NO: 2018; SEQ ID NO: 2019; SEQ ID NO: 2020; SEQ ID NO: 2021; SEQ ID NO: 2022; SEQ ID NO: 2023; SEQ ID NO: 2024; SEQ ID NO: 2025; SEQ ID NO: 2026; SEQ ID NO: 2027; SEQ ID NO: 2028; SEQ ID NO: 2029; SEQ ID NO: 2030; SEQ ID NO: 2031; SEQ ID NO: 2032; SEQ ID NO: 2033; SEQ ID NO: 2034; SEQ ID NO: 2035; SEQ ID NO: 2036; SEQ ID NO: 2037; SEQ ID NO: 2038; SEQ ID NO: 2039; SEQ ID NO: 2040; SEQ ID NO: 2041; SEQ ID NO: 2042; SEQ ID NO: 2043; SEQ ID NO: 2044; SEQ ID NO: 2045; SEQ ID NO: 2046; SEQ ID NO: 2047; SEQ ID NO: 2048; SEQ ID NO: 2049; SEQ ID NO: 2050; SEQ ID NO: 2051; SEQ ID NO: 2052; SEQ ID NO: 2053; SEQ ID NO: 2054; SEQ ID NO: 2055; SEQ ID NO: 2056; SEQ ID NO: 2057; SEQ ID NO: 2058; SEQ ID NO: 2059; SEQ ID NO: 2060; SEQ ID NO: 2061; SEQ ID NO: 2062; SEQ ID NO: 2063; SEQ ID NO: 2064; SEQ ID NO: 2065; SEQ ID NO: 2066; SEQ ID NO: 2067; SEQ ID NO: 2068; SEQ ID NO: 2069; SEQ ID NO: 2070; SEQ ID NO: 2071; SEQ ID NO: 2072; SEQ ID NO: 2073; SEQ ID NO: 2074; SEQ ID NO: 2075; SEQ ID NO: 2076; and SEQ ID NO: 9598; SEQ ID NO: 9599, or a complement thereof.  
     [0079] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in nucleic acid metabolism encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2077; SEQ ID NO: 2078; SEQ ID NO: 2079; SEQ ID NO: 2080; SEQ ID NO: 2081; SEQ ID NO: 2082; SEQ ID NO: 2083; SEQ ID NO: 2084; SEQ ID NO: 2085; SEQ ID NO: 2086; SEQ ID NO: 2087; SEQ ID NO: 2088; SEQ ID NO: 2089; SEQ ID NO: 2090; SEQ ID NO: 2091; SEQ ID NO: 2092; SEQ ID NO: 2093; SEQ ID NO: 2094; SEQ ID NO: 2095; SEQ ID NO: 2096; SEQ ID NO: 2097; SEQ ID NO: 2098; SEQ ID NO: 2099; SEQ ID NO: 2100; SEQ ID NO: 2101; SEQ ID NO: 2102; SEQ ID NO: 2103; SEQ ID NO: 2104; SEQ ID NO: 2105; SEQ ID NO: 2106; SEQ ID NO: 2107; SEQ ID NO: 2108; SEQ ID NO: 2109; SEQ ID NO: 2110; SEQ ID NO: 2111; SEQ ID NO: 2112; SEQ ID NO: 2113; SEQ ID NO: 2114; SEQ ID NO:2115; SEQ ID NO:2116; SEQ ID NO:2117; SEQ ID NO:2118; SEQ ID NO: 2119; SEQ ID NO: 2120; SEQ ID NO: 2121; SEQ ID NO: 2122; SEQ ID NO: 2123; SEQ ID NO: 2124; SEQ ID NO: 2125; SEQ ID NO: 2126; SEQ ID NO: 2127; SEQ ID NO: 2128; SEQ ID NO: 2129; SEQ ID NO: 2130; SEQ ID NO: 2131; SEQ ID NO: 2132; SEQ ID NO: 2133; SEQ ID NO: 2134; SEQ ID NO: 2135; SEQ ID NO: 2136; SEQ ID NO: 2137; SEQ ID NO: 2138; SEQ ID NO: 2139; SEQ ID NO: 2140; SEQ ID NO: 2141; SEQ ID NO: 2142; SEQ ID NO: 2143; SEQ ID NO: 2144; SEQ ID NO: 2145; SEQ ID NO: 2146; SEQ ID NO: 2147; SEQ ID NO: 2148; SEQ ID NO: 2149; SEQ ID NO: 2150; SEQ ID NO: 2151; SEQ ID NO: 2152; SEQ ID NO: 2153; SEQ ID NO: 2154; SEQ ID NO: 2155; SEQ ID NO: 2156; SEQ ID NO: 2157; SEQ ID NO: 2158; SEQ ID NO:2159; SEQ ID NO:2160; SEQ ID NO:2161; SEQ ID NO: 2162; SEQ ID NO: 2163; SEQ ID NO: 2164; SEQ ID NO: 2165; SEQ ID NO: 2166; SEQ ID NO: 2167; SEQ ID NO: 2168; SEQ ID NO: 2169; SEQ ID NO: 2170; SEQ ID NO: 2171; SEQ ID NO: 2172; SEQ ID NO: 2173; SEQ ID NO: 2174; SEQ ID NO: 2175; SEQ ID NO: 2176; SEQ ID NO: 2177; SEQ ID NO: 2178; SEQ ID NO: 2179; SEQ ID NO: 2180; SEQ ID NO: 2181; SEQ ID NO: 2182; and SEQ ID NO: 9600, or a complement thereof.  
     [0080] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in lipid metabolism encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2183; SEQ ID NO: 2184; SEQ ID NO: 2185; SEQ ID NO: 2186; SEQ ID NO: 2187; SEQ ID NO: 2188; SEQ ID NO: 2189; SEQ ID NO: 2190; SEQ ID NO: 2191; SEQ ID NO: 2192; SEQ ID NO: 2193; SEQ ID NO: 2194; SEQ ID NO 2195; SEQ ID NO: 2196; SEQ ID NO: 2197; SEQ ID NO: 2198; SEQ ID NO: 2199; SEQ ID NO: 2200; SEQ ID NO: 2201; SEQ ID NO: 2202; SEQ ID NO: 2203; SEQ ID NO: 2204; SEQ ID NO: 2205; SEQ ID NO: 2206; SEQ ID NO: 2207; SEQ ID NO: 2208; SEQ ID NO: 2209; SEQ ID NO: 2210; SEQ ID NO: 2211; SEQ ID NO: 2212; SEQ ID NO: 2213; SEQ ID NO: 2214; SEQ ID NO: 2215; SEQ ID NO: 2216; SEQ ID NO: 2217; SEQ ID NO: 2218; and SEQ ID NO: 9601; SEQ ID NO: 9602, or a complement thereof.  
     [0081] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in mRNA translation and ribosome biogenesis encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2219; SEQ ID NO: 2220; SEQ ID NO: 2221; SEQ ID NO: 2222; SEQ ID NO: 2223; SEQ ID NO: 2224; SEQ ID NO: 2225; SEQ ID NO: 2226; SEQ ID NO: 2227; SEQ ID NO: 2228; SEQ ID NO: 2229; SEQ ID NO: 2230; SEQ ID NO: 2231; SEQ ID NO: 2232; SEQ ID NO: 2233; SEQ ID NO: 2234; SEQ ID NO: 2235; SEQ ID NO: 2236; SEQ ID NO: 2237; SEQ ID NO: 2238; SEQ ID NO: 2239; SEQ ID NO: 2240; SEQ ID NO: 2241; SEQ ID NO: 2242; SEQ ID NO: 2243; SEQ ID NO: 2244; SEQ ID NO: 2245; SEQ ID NO: 2246; SEQ ID NO: 2247; SEQ ID NO: 2248; SEQ ID NO: 2249; SEQ ID NO: 2250; SEQ ID NO: 2251; SEQ ID NO: 2252; SEQ ID NO: 2253; SEQ ID NO: 2254; SEQ ID NO: 2255; SEQ ID NO: 2256; SEQ ID NO: 2257; SEQ ID NO: 2258; SEQ ID NO: 2259; SEQ ID NO: 2260; SEQ ID NO: 2261; SEQ ID NO: 2262; SEQ ID NO: 2263; SEQ ID NO: 2264; SEQ ID NO: 2265; SEQ ID NO: 2266; SEQ ID NO: 2267; SEQ ID NO: 2268; SEQ ID NO: 2269; SEQ ID NO: 2270; SEQ ID NO: 2271; SEQ ID NO: 2272; SEQ ID NO: 2273; SEQ ID NO: 2274; SEQ ID NO: 2275; SEQ ID NO: 2276; SEQ ID NO: 2277; SEQ ID NO: 2278; SEQ ID NO: 2279; SEQ ID NO: 2280; SEQ ID NO: 2281; SEQ ID NO: 2282; SEQ ID NO: 2283; SEQ ID NO: 2284; SEQ ID NO: 2285; SEQ ID NO: 2286; SEQ ID NO: 2287; SEQ ID NO: 2288; SEQ ID NO: 2289; SEQ ID NO: 2290; SEQ ID NO: 2291; SEQ ID NO: 2292; SEQ ID NO: 2293; SEQ ID NO: 2294; SEQ ID NO: 2295; SEQ ID NO: 2296; SEQ ID NO: 2297; SEQ ID NO: 2298; SEQ ID NO: 2299; SEQ ID NO: 2300; SEQ ID NO: 2301; SEQ ID NO: 2302; SEQ ID NO: 2303; SEQ ID NO: 2304; SEQ ID NO: 2305; SEQ ID NO: 2306; SEQ ID NO: 2307; SEQ ID NO: 2308; SEQ ID NO: 2309; SEQ ID NO: 2310; SEQ ID NO: 2311; SEQ ID NO: 2312; SEQ ID NO: 2313; SEQ ID NO: 2314; SEQ ID NO: 2315; SEQ ID NO: 2316; SEQ ID NO: 2317; SEQ ID NO: 2318; SEQ ID NO: 2319; SEQ ID NO: 2320; SEQ ID NO: 2321; SEQ ID NO: 2322; SEQ ID NO: 2323; SEQ ID NO: 2324; SEQ ID NO: 2325; SEQ ID NO: 2326; SEQ ID NO: 2327; SEQ ID NO: 2328; SEQ ID NO: 2329; SEQ ID NO: 2330; SEQ ID NO: 2331; SEQ ID NO: 2332; SEQ ID NO: 2333; SEQ ID NO: 2334; SEQ ID NO: 2335; SEQ ID NO: 2336; SEQ ID NO: 2337; SEQ ID NO: 2338; SEQ ID NO: 2339; SEQ ID NO: 2340; SEQ ID NO: 2341; SEQ ID NO: 2342; SEQ ID NO: 2343; SEQ ID NO: 2344; SEQ ID NO: 2345; SEQ ID NO: 2346; SEQ ID NO: 2347; SEQ ID NO: 2348; SEQ ID NO: 2349; SEQ ID NO: 2350; SEQ ID NO: 2351; SEQ ID NO: 2352; SEQ ID NO: 2353; SEQ ID NO: 2354; SEQ ID NO: 2355; SEQ ID NO: 2356; SEQ ID NO: 2357; SEQ ID NO: 2358; SEQ ID NO: 2359; SEQ ID NO: 2360; SEQ ID NO: 2361; SEQ ID NO: 2362; SEQ ID NO: 2363; SEQ ID NO: 2364; SEQ ID NO: 2365; SEQ ID NO: 2366; SEQ ID NO: 2367; SEQ ID NO: 2368; SEQ ID NO: 2369; SEQ ID NO: 2370; SEQ ID NO: 2371; SEQ ID NO: 2372; SEQ ID NO: 2373; SEQ ID NO: 2374; SEQ ID NO: 2375; SEQ ID NO: 2376; SEQ ID NO: 2377; SEQ ID NO: 2378; SEQ ID NO: 2379; SEQ ID NO: 2380; SEQ ID NO: 2381; SEQ ID NO: 2382; SEQ ID NO: 2383; SEQ ID NO: 2384; SEQ ID NO: 2385; SEQ ID NO: 2386; SEQ ID NO: 2387; SEQ ID NO: 2388; SEQ ID NO: 2389; SEQ ID NO: 2390; SEQ ID NO: 2391; SEQ ID NO: 2392; SEQ ID NO: 2393; SEQ ID NO: 2394; SEQ ID NO: 2395; SEQ ID NO: 2396; SEQ ID NO: 2397; SEQ ID NO: 2398; SEQ ID NO: 2399; SEQ ID NO: 2400; SEQ ID NO: 2401; SEQ ID NO: 2402; SEQ ID NO: 2403; SEQ ID NO: 2404; SEQ ID NO: 2405; SEQ ID NO: 2406; SEQ. ID NO: 2407; SEQ ID NO: 2408; SEQ ID NO: 2409; SEQ ID NO: 2410; SEQ ID NO: 2411; SEQ ID NO: 2412; SEQ ID NO: 2413; SEQ ID NO: 2414; SEQ ID NO: 2415; SEQ ID NO: 2416; SEQ ID NO: 2417; SEQ ID NO: 2418; SEQ ID NO: 2419; SEQ ID NO: 2420; SEQ ID NO: 2421; SEQ ID NO: 2422; SEQ ID NO: 2423; SEQ ID NO: 2424; SEQ ID NO: 2425; SEQ ID NO: 2426; SEQ ID NO: 2427; SEQ ID NO: 2428; SEQ ID NO: 2429; SEQ ID NO: 2430; SEQ ID NO: 2431; SEQ ID NO: 2432; SEQ ID NO: 2433; SEQ ID NO: 2434; SEQ ID NO: 2435; SEQ ID NO: 2436; SEQ ID NO: 2437; SEQ ID NO: 2438 and SEQ ID NO: 9603; SEQ ID NO: 9604, or a complement thereof.  
     [0082] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in genome replication, transcription, recombination and repair encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2439; SEQ ID NO: 2440; SEQ ID NO: 2441; SEQ ID NO: 2442; SEQ ID NO: 2443; SEQ ID NO: 2444; SEQ ID NO: 2445; SEQ ID NO: 2446; SEQ ID NO: 2447; SEQ ID NO: 2448; SEQ ID NO: 2449; SEQ ID NO: 2450; SEQ ID NO: 2451; SEQ ID NO: 2452; SEQ ID NO: 2453; SEQ ID NO: 2454; SEQ ID NO: 2455; SEQ ID NO: 2456; SEQ ID NO: 2457; SEQ ID NO: 2458; SEQ ID NO: 2459; SEQ ID NO: 2460; SEQ ID NO: 2461; SEQ ID NO: 2462; SEQ ID NO: 2463; SEQ ID NO: 2464; SEQ ID NO: 2465; SEQ ID NO: 2466; SEQ ID NO: 2467; SEQ ID NO: 2468; SEQ ID NO: 2469; SEQ ID NO: 2470; SEQ ID NO: 2471; SEQ ID NO: 2472; SEQ ID NO: 2473; SEQ ID NO: 2474; SEQ ID NO: 2475; SEQ ID NO: 2476; SEQ ID NO: 2477; SEQ ID NO: 2478; SEQ ID NO: 2479; SEQ ID NO: 2480; SEQ ID NO: 2481; SEQ ID NO: 2482; SEQ ID NO: 2483; SEQ ID NO: 2484; SEQ ID NO: 2485; SEQ ID NO: 2486; SEQ ID NO: 2487; SEQ ID NO: 2488; SEQ ID NO: 2489; SEQ ID NO: 2490; SEQ ID NO: 2491; SEQ ID NO: 2492; SEQ ID NO: 2493; SEQ ID NO: 2494; SEQ ID NO: 2495; SEQ ID NO: 2496; SEQ ID NO: 2497; SEQ ID NO: 2498; SEQ ID NO: 2499; SEQ ID NO: 2500; SEQ ID NO: 2501; SEQ ID NO: 2502; SEQ ID NO: 2503; SEQ ID NO: 2504; SEQ ID NO: 2505; SEQ ID NO: 2506; SEQ ID NO: 2507; SEQ ID NO: 2508; SEQ ID NO: 2509; SEQ ID NO: 2510; SEQ ID NO: 2511; SEQ ID NO: 2512; SEQ ID NO: 2513; SEQ ID NO: 2514; SEQ ID NO: 2515; SEQ ID NO: 2516; SEQ ID NO: 2517; SEQ ID NO: 2518; SEQ ID NO: 2519; SEQ ID NO: 2520; SEQ ID NO: 2521; SEQ ID NO: 2522; SEQ ID NO: 2523; SEQ ID NO: 2524; SEQ ID NO: 2525; SEQ ID NO: 2526; SEQ ID NO: 2527; SEQ ID NO: 2528; SEQ ID NO: 2529; SEQ ID NO: 2530; SEQ ID NO: 2531; SEQ ID NO: 2532; SEQ ID NO: 2533; SEQ ID NO: 2534; SEQ ID NO: 2535; SEQ ID NO: 2536; SEQ ID NO: 2537; SEQ ID NO: 2538; SEQ ID NO: 2539; SEQ ID NO: 2540; SEQ ID NO: 2541; SEQ ID NO: 2542; SEQ ID NO: 2543; SEQ ID NO: 2544; SEQ ID NO: 2545; SEQ ID NO: 2546; SEQ ID NO: 2547; SEQ ID NO: 2548; SEQ ID NO: 2549; SEQ ID NO: 2550; SEQ ID NO: 2551; SEQ ID NO: 2552; SEQ ID NO: 2553; SEQ ID NO: 2554; SEQ ID NO: 2555; SEQ ID NO: 2556; SEQ ID NO: 2557; SEQ ID NO: 2558; SEQ ID NO: 2559; SEQ ID NO: 2560; SEQ ID NO: 2561; SEQ ID NO: 2562; SEQ ID NO: 2563; SEQ ID NO: 2564; SEQ ID NO: 2565; SEQ ID NO: 2566; SEQ ID NO: 2567; SEQ ID NO: 2568; SEQ ID NO: 2569; SEQ ID NO: 2570; SEQ ID NO: 2571; SEQ ID NO: 2572; SEQ ID NO: 2573; SEQ ID NO: 2574; SEQ ID NO: 2575; SEQ ID NO: 2576; SEQ ID NO: 2577; SEQ ID NO: 2578; SEQ ID NO: 2579; SEQ ID NO: 2580; SEQ ID NO: 2581; SEQ ID NO: 2582; SEQ ID NO: 2583; SEQ ID NO: 2584; SEQ ID NO: 2585; SEQ ID NO: 2586; SEQ ID NO: 2587; SEQ ID NO: 2588; SEQ ID NO: 2589; SEQ ID NO: 2590; SEQ ID NO: 2591; SEQ ID NO: 2592; SEQ ID NO: 2593; SEQ ID NO: 2594; SEQ ID NO: 2595; SEQ ID NO: 2596; SEQ ID NO: 2597; SEQ ID NO: 2598; SEQ ID NO: 2599; SEQ ID NO: 2600; SEQ ID NO: 2601; SEQ ID NO: 2602; SEQ ID NO: 2603; SEQ ID NO: 2604; SEQ ID NO: 2605; SEQ ID NO: 2606; SEQ ID NO: 2607; SEQ ID NO: 2608; SEQ ID NO: 2609; SEQ ID NO: 2610; SEQ ID NO: 2611; SEQ ID NO: 2612; SEQ ID NO: 2613; SEQ ID NO: 2614; SEQ ID NO: 2615; SEQ ID NO: 2616; SEQ ID NO: 2617; SEQ ID NO: 2618; SEQ ID NO: 2619; SEQ ID NO: 2620; SEQ ID NO: 2621; SEQ ID NO: 2622; SEQ ID NO: 2623; SEQ ID NO: 2624; SEQ ID NO: 2625; SEQ ID NO: 2626; SEQ ID NO: 2627; SEQ ID NO: 2628; SEQ ID NO: 2629; SEQ ID NO: 2630; SEQ ID NO: 2631; SEQ ID NO: 2632; SEQ ID NO: 2633; SEQ ID NO: 2634; SEQ ID NO: 2635; SEQ ID NO: 2636; SEQ ID NO: 2637; SEQ ID NO: 2638; SEQ ID NO: 2639; SEQ ID NO: 2640; SEQ ID NO:2641; SEQ ID NO: 2642; SEQ ID NO: 2643; SEQ ID NO: 2644; SEQ ID NO: 2645; SEQ ID NO: 2646; SEQ ID NO: 2647; SEQ ID NO: 2648; SEQ ID NO: 2649; SEQ ID NO: 2650; SEQ ID NO: 2651; SEQ ID NO: 2652; SEQ ID NO: 2653; SEQ ID NO: 2654; SEQ ID NO: 2655; SEQ ID NO: 2656; SEQ ID NO: 2657; SEQ ID NO: 2658; SEQ ID NO: 2659; SEQ ID NO: 2660; SEQ ID NO: 2661; SEQ ID NO: 2662; SEQ ID NO: 2663; SEQ ID NO: 2664; SEQ ID NO: 2665; SEQ ID NO: 2666; SEQ ID NO: 2667; SEQ ID NO: 2668; SEQ ID NO: 2669; SEQ ID NO: 2670; SEQ ID NO: 2671; SEQ ID NO: 2672; SEQ ID NO: 2673; SEQ ID NO: 2674; SEQ ID NO: 2675; SEQ ID NO: 2676; SEQ ID NO: 2677; SEQ ID NO: 2678; SEQ ID NO: 2679; SEQ ID NO: 2680; SEQ ID NO: 2681; SEQ ID NO: 2682; SEQ ID NO: 2683; SEQ ID NO: 2684; SEQ ID NO: 2685; SEQ ID NO: 2686; SEQ ID NO: 2687; SEQ ID NO: 2688; SEQ ID NO: 2689; SEQ ID NO: 2690; SEQ ID NO: 2691; SEQ ID NO: 2692; SEQ ID NO: 2693; SEQ ID NO: 2694; SEQ ID NO: 2695; SEQ ID NO: 2696; SEQ ID NO: 2697; SEQ ID NO: 2698; SEQ ID NO: 2699; SEQ ID NO: 2700; SEQ ID NO: 2701; SEQ ID NO: 2702; SEQ ID NO: 2703; SEQ ID NO: 2704; SEQ ID NO: 2705; SEQ ID NO: 2706; SEQ ID NO: 2707; SEQ ID NO: 2708; SEQ ID NO: 2709; SEQ ID NO: 2710; SEQ ID NO: 2711; SEQ ID NO: 2712; SEQ ID NO: 2713; SEQ ID NO: 2714; SEQ ID NO: 2715; SEQ ID NO: 2716; SEQ ID NO: 2717; SEQ ID NO: 2718; SEQ ID NO: 2719; SEQ ID NO: 2720; SEQ ID NO: 2721; SEQ ID NO: 2722; SEQ ID NO: 2723; SEQ ID NO: 2724; SEQ ID NO: 2725; SEQ ID NO: 2726; SEQ ID NO: 2727; SEQ ID NO: 2728; SEQ ID NO: 2729; SEQ ID NO: 2730; SEQ ID NO: 2731; SEQ ID NO: 2732; SEQ ID NO: 2733; SEQ ID NO: 2734; SEQ ID NO: 2735; SEQ ID NO: 2736; SEQ ID NO: 2737; SEQ ID NO: 2738; SEQ ID NO: 2739; SEQ ID NO: 2740; SEQ ID NO: 2741; SEQ ID NO: 2742; SEQ ID NO: 2743; SEQ ID NO: 2744; SEQ ID NO: 2745; SEQ ID NO: 2746; SEQ ID NO: 2747; SEQ ID NO: 2748; SEQ ID NO: 2749; SEQ ID NO: 2750; SEQ ID NO: 2751; SEQ ID NO: 2752; SEQ ID NO: 2753; SEQ ID NO: 2754; SEQ ID NO: 2755; SEQ ID NO: 2756; SEQ ID NO: 2757; SEQ ID NO: 2758; SEQ ID NO: 2759; SEQ ID NO: 2760; SEQ ID NO: 2761; SEQ ID NO: 2762; SEQ ID NO: 2763; SEQ ID NO: 2764; SEQ ID NO: 2765; SEQ ID NO: 2766; SEQ ID NO: 2767; SEQ ID NO: 2768; SEQ ID NO: 2769; SEQ ID NO: 2770; SEQ ID NO: 2771; SEQ ID NO: 2772; SEQ ID NO: 2773; SEQ ID NO: 2774; SEQ ID NO: 2775; SEQ ID NO: 2776; SEQ ID NO: 2777; SEQ ID NO: 2778; SEQ ID NO: 2779; SEQ ID NO: 2780; SEQ ID NO: 2781; SEQ ID NO: 2782; SEQ ID NO: 2783; SEQ ID NO: 2784; SEQ ID NO: 2785; SEQ ID NO: 2786; SEQ ID NO: 2787; SEQ ID NO: 2788; SEQ ID NO: 2789; SEQ ID NO: 2790; SEQ ID NO: 2791; SEQ ID NO: 2792; SEQ ID NO: 2793; SEQ ID NO: 2794; SEQ ID NO: 2795; SEQ ID NO: 2796; SEQ ID NO: 2797; SEQ ID NO: 2798; SEQ ID NO: 2799; SEQ ID NO: 2800; SEQ ID NO: 2801; SEQ ID NO: 2802; SEQ ID NO: 2803; SEQ ID NO: 2804; SEQ ID NO: 2805; SEQ ID NO: 2806; SEQ ID NO: 2807; and SEQ ID NO: 9605; SEQ ID NO: 9606; SEQ ID NO: 9607 and SEQ ID NO: 9608, or a complement thereof.  
     [0083] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in secretion and adhesion encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2808; SEQ ID NO: 2809; SEQ ID NO: 2810; SEQ ID NO: 2811; SEQ ID NO: 2812; SEQ ID NO: 2813; SEQ ID NO: 2814; SEQ ID NO: 2815; SEQ ID NO: 2816; SEQ ID NO: 2817; SEQ ID NO: 2818; SEQ ID NO: 2819; SEQ ID NO: 2820; SEQ ID NO: 2821; SEQ ID NO: 2822; SEQ ID NO: 2823 and SEQ ID NO: 2824 or a complement thereof.  
     [0084] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in outer membrane and cell wall biogenesis encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2825; SEQ ID NO: 2826; SEQ ID NO: 2827; SEQ ID NO: 2828; SEQ ID NO: 2829; SEQ ID NO: 2830; SEQ ID NO: 2831; SEQ ID NO: 2832; SEQ ID NO: 2833; SEQ ID NO: 2834; SEQ ID NO:2835; SEQ ID NO: 2836; SEQ ID NO: 2837; SEQ ID NO: 2838; SEQ ID NO: 2839; SEQ ID NO: 2840; SEQ ID NO: 2841; SEQ ID NO: 2842; SEQ ID NO: 2843; SEQ ID NO: 2844; SEQ ID NO: 2845; SEQ ID NO: 2846; SEQ ID NO: 2847; SEQ ID NO: 2848; SEQ ID NO: 2849; SEQ ID NO: 2850; SEQ ID NO: 2851; SEQ ID NO: 2852; SEQ ID NO: 2853; SEQ ID NO: 2854; SEQ ID NO: 2855; SEQ ID NO: 2856; SEQ ID NO: 2857; SEQ ID NO: 2858; SEQ ID NO: 2859; SEQ ID NO: 2860; SEQ ID NO: 2861; SEQ ID NO: 2862; SEQ ID NO: 2863; SEQ ID NO: 2864; SEQ ID NO: 2865; SEQ ID NO: 2866; SEQ ID NO: 2867; SEQ ID NO: 2868; SEQ ID NO: 2869; SEQ ID NO: 2870; SEQ ID NO: 2871; SEQ ID NO: 2872; SEQ ID NO: 2873; SEQ ID NO: 2874; SEQ ID NO: 2875; SEQ ID NO: 2876; SEQ ID NO: 2877; and SEQ ID NO: 9609 and SEQ ID NO: 9610, or a complement thereof.  
     [0085] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in protein folding and stabilization encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2878; SEQ ID NO: 2879; SEQ ID NO: 2880; SEQ ID NO: 2881; SEQ ID NO: 2882; SEQ ID NO: 2883; SEQ ID NO: 2884; SEQ ID NO: 2885; SEQ ID NO: 2886; SEQ ID NO: 2887; SEQ ID NO: 2888; SEQ ID NO: 2889; SEQ ID NO: 2890; SEQ ID NO: 2891; SEQ ID NO: 2892; SEQ ID NO: 2893; SEQ ID NO: 2894; SEQ ID NO: 2895; SEQ ID NO: 2896; SEQ ID NO: 2897; SEQ ID NO: 2898; SEQ ID NO: 2899; SEQ ID NO: 2900; SEQ ID NO: 2901; SEQ ID NO: 2902; SEQ ID NO: 2903; SEQ ID NO: 2904; SEQ ID NO: 2905; SEQ ID NO: 2906; SEQ ID NO: 2907; SEQ ID NO: 2908; SEQ ID NO: 2909; SEQ ID NO: 2910; SEQ ID NO: 2911; SEQ ID NO: 2912; SEQ ID NO: 2913; SEQ ID NO: 2914; SEQ ID NO: 2915; SEQ ID NO: 2916; SEQ ID NO: 2917; SEQ ID NO: 2918; and SEQ ID NO: 9611 and SEQ ID NO: 9612, or a complement thereof.  
     [0086] Particularly preferred is an isolated nucleic acid having a nucleotide sequence encoding an  H. pylori  secreted polypeptide or a fragment thereof, the nucleic acid selected from the group consisting of SEQ ID NO: 4016; SEQ ID NO: 4017; SEQ ID NO: 4018; SEQ ID NO: 4019; SEQ ID NO: 4020; SEQ ID NO: 4021; SEQ ID NO: 4022; SEQ ID NO: 4023; SEQ ID NO: 4024; SEQ ID NO: 4025; SEQ ID NO: 4026; SEQ ID NO: 4027; SEQ ID NO: 4028; SEQ ID NO: 4029; SEQ ID NO: 4030; SEQ ID NO: 4031; SEQ ID NO: 4032; SEQ ID NO: 4033; SEQ ID NO: 4034; SEQ ID NO: 4035; SEQ ID NO: 4036; SEQ ID NO: 4037; SEQ ID NO: 4038; SEQ ID NO: 4039; SEQ ID NO: 4040; SEQ ID NO: 4041; SEQ ID NO: 4042; SEQ ID NO: 4043; SEQ ID NO: 4044; SEQ ID NO: 4045; SEQ ID NO: 4046; SEQ ID NO: 4047; SEQ ID NO: 4048; SEQ ID NO: 4049; SEQ ID NO: 4050; SEQ ID NO: 4051; SEQ ID NO: 4052; SEQ ID NO: 4053; SEQ ID NO: 4054; SEQ ID NO: 4055; SEQ ID NO: 4056; SEQ ID NO: 4057; SEQ ID NO: 4058; SEQ ID NO: 4059; SEQ ID NO: 4060; SEQ ID NO: 4061; SEQ ID NO: 4062; SEQ ID NO: 4063; SEQ ID NO: 4064; SEQ ID NO: 4065; SEQ ID NO: 4066; SEQ ID NO: 4067; SEQ ID NO: 4068; SEQ ID NO: 4069; SEQ ID NO: 4070; SEQ ID NO: 4071; SEQ ID NO: 4072; SEQ ID NO: 4073; SEQ ID NO: 4074; SEQ ID NO: 4075; SEQ ID NO: 4076; SEQ ID NO: 4077; SEQ ID NO: 4078; SEQ ID NO: 4079; SEQ ID NO: 4080; SEQ ID NO: 4081; SEQ ID NO: 4082; SEQ ID NO: 4083; SEQ ID NO: 4084; SEQ ID NO: 4085; SEQ ID NO: 4086; SEQ ID NO: 4087; SEQ ID NO: 4088; SEQ ID NO: 4089; SEQ ID NO: 4090; SEQ ID NO: 4091; SEQ ID NO: 4092; SEQ ID NO: 4093; SEQ ID NO: 4094; SEQ ID NO: 4095; SEQ ID NO: 4096; SEQ ID NO: 4097; SEQ ID NO: 4098; SEQ ID NO: 4099; SEQ ID NO: 4100; SEQ ID NO: 4101; SEQ ID NO: 4102; SEQ ID NO: 4103; SEQ ID NO: 4104; SEQ ID NO: 4105; SEQ ID NO: 4106; SEQ ID NO: 4107; SEQ ID NO: 4108; SEQ ID NO: 4109; SEQ ID NO: 4110; SEQ ID NO: 4111; SEQ ID NO: 4112; SEQ ID NO: 4113; SEQ ID NO: 4114; SEQ ID NO: 4115; SEQ ID NO: 4116; SEQ ID NO: 4117; SEQ ID NO: 4118; SEQ ID NO: 4119; SEQ ID NO: 4120; SEQ ID NO: 4121; SEQ ID NO: 4122; SEQ ID NO: 4123; SEQ ID NO: 4124; SEQ ID NO: 4125; SEQ ID NO: 4126; SEQ ID NO: 4127; SEQ ID NO: 4128; SEQ ID NO: 4129; SEQ ID NO: 4130; SEQ ID NO: 4131; SEQ ID NO: 4132; SEQ ID NO: 4133; SEQ ID NO: 4134; SEQ ID NO: 4135; SEQ ID NO: 4136; SEQ ID NO: 4137; SEQ ID NO: 4138; SEQ ID NO: 4139; SEQ ID NO: 4140; SEQ ID NO: 4141; SEQ ID NO: 4142; SEQ ID NO: 4143; SEQ ID NO: 4144; SEQ ID NO: 4145; SEQ ID NO: 4146; SEQ ID NO: 4147; SEQ ID NO: 4148; SEQ ID NO: 4149; SEQ ID NO: 4150; SEQ ID NO: 4151; SEQ ID NO: 4152; SEQ ID NO: 4153; SEQ ID NO: 4154; SEQ ID NO: 4155; SEQ ID NO: 4156; SEQ ID NO: 4157; SEQ ID NO: 4158; SEQ ID NO: 4159; SEQ ID NO: 4160; SEQ ID NO: 4161; SEQ ID NO: 4162; SEQ ID NO: 4163; SEQ ID NO: 4164; SEQ ID NO: 4165; SEQ ID NO: 4166; SEQ ID NO: 4167; SEQ ID NO: 4168; SEQ ID NO: 4169; SEQ ID NO: 4170; SEQ ID NO: 4171; SEQ ID NO: 4172; SEQ ID NO: 4173; SEQ ID NO: 4174; SEQ ID NO: 4175; SEQ ID NO: 4176; SEQ ID NO: 4177; SEQ ID NO: 4178; SEQ ID NO: 4179; SEQ ID NO: 4180; SEQ ID NO: 4181; SEQ ID NO: 4182; SEQ ID NO: 4183; SEQ ID NO: 4184; SEQ ID NO: 4185; SEQ ID NO: 4186; SEQ ID NO: 4187; SEQ ID NO: 4188; SEQ ID NO: 4189; SEQ ID NO: 4190; SEQ ID NO: 4191; SEQ ID NO: 4192; SEQ ID NO: 4193; SEQ ID NO: 4194; SEQ ID NO: 4195; SEQ ID NO: 4196; SEQ ID NO: 4197; SEQ ID NO: 4198; SEQ ID NO: 4199; SEQ ID NO: 4200; SEQ ID NO: 4201; SEQ ID NO: 4202; SEQ ID NO: 4203; SEQ ID NO: 4204; SEQ ID NO: 4205; SEQ ID NO: 4206; SEQ ID NO: 4207; SEQ ID NO: 4208; SEQ ID NO: 4209; SEQ ID NO: 4210; SEQ ID NO: 4211; SEQ ID NO: 4212; SEQ ID NO: 4213; SEQ ID NO: 4214; SEQ ID NO: 4215; SEQ ID NO: 4216; SEQ ID NO: 4217; SEQ ID NO: 4218; SEQ ID NO: 4219; SEQ ID NO: 4220; SEQ ID NO: 4221; SEQ ID NO: 4222; SEQ ID NO: 4223; SEQ ID NO: 4224; SEQ ID NO: 4225; SEQ ID NO: 4226; SEQ ID NO: 4227; SEQ ID NO: 4228; SEQ ID NO: 4229; SEQ ID NO: 4230; SEQ ID NO: 4231; SEQ ID NO: 4232; SEQ ID NO: 4233; SEQ ID NO: 4234; SEQ ID NO: 4235; SEQ ID NO: 4236; SEQ ID NO: 4237; SEQ ID NO: 4238; SEQ ID NO: 4239; SEQ ID NO: 4240; SEQ ID NO: 4241; SEQ ID NO: 4242; SEQ ID NO: 4243; SEQ ID NO: 4244; SEQ ID NO: 4245; SEQ ID NO: 4246; SEQ ID NO: 4247; SEQ ID NO: 4248; SEQ ID NO: 4249; SEQ ID NO: 4250; SEQ ID NO: 4251; SEQ ID NO: 4252; SEQ ID NO: 4253; SEQ ID NO: 4254; SEQ ID NO: 4255; SEQ ID NO: 4256; SEQ ID NO: 4257; SEQ ID NO: 4258; SEQ ID NO: 4259; SEQ ID NO: 4260; SEQ ID NO: 4261; SEQ ID NO: 4262; SEQ ID NO: 4263; SEQ ID NO: 4264; SEQ ID NO: 4265; SEQ ID NO: 4266; SEQ ID NO: 4267; SEQ ID NO: 4268; SEQ ID NO: 4269; SEQ ID NO: 4270; SEQ ID NO: 4271; SEQ ID NO: 4272; SEQ ID NO: 4273; SEQ ID NO: 4274; SEQ ID NO: 4275; SEQ ID NO: 4276; SEQ ID NO: 4277; SEQ ID NO: 4278; SEQ ID NO: 4279; SEQ ID NO: 4280; SEQ ID NO: 4281; SEQ ID NO: 4282; SEQ ID NO: 4283; SEQ ID NO: 4284; SEQ ID NO: 4285; SEQ ID NO: 4286; SEQ ID NO: 4287; SEQ ID NO: 4288; SEQ ID NO: 4289; SEQ ID NO: 4290; SEQ ID NO: 4291; SEQ ID NO: 4292; SEQ ID NO: 4293; SEQ ID NO: 4294; SEQ ID NO: 4295; SEQ ID NO: 4296; SEQ ID NO: 4297; SEQ ID NO: 4298; SEQ ID NO: 4299; SEQ ID NO: 4300; SEQ ID NO: 4301; SEQ ID NO: 4302; SEQ ID NO: 4303; SEQ ID NO: 4304; SEQ ID NO: 4305; SEQ ID NO: 4306; SEQ ID NO: 4307; SEQ ID NO: 4308; SEQ ID NO: 4309; SEQ ID NO: 4310; SEQ ID NO: 4311; SEQ ID NO: 4312; SEQ ID NO: 4313; SEQ ID NO: 4314; SEQ ID NO: 4315; SEQ ID NO: 4316; SEQ ID NO: 4317; SEQ ID NO: 4318; SEQ ID NO: 4319; SEQ ID NO: 4320; SEQ ID NO: 4321; SEQ ID NO: 4322; SEQ ID NO: 4323; SEQ ID NO: 4324; SEQ ID NO: 4325; SEQ ID NO: 4326; SEQ ID NO: 4327; SEQ ID NO: 4328; SEQ ID NO: 4329; SEQ ID NO: 4330; SEQ ID NO: 4331; SEQ ID NO: 4332; SEQ ID NO: 4333; SEQ ID NO: 4334; SEQ ID NO: 4335; SEQ ID NO: 4336; SEQ ID NO: 4337; SEQ ID NO: 4338; SEQ ID NO: 4339; SEQ ID NO: 4340; SEQ ID NO: 4341; SEQ ID NO: 4342; SEQ ID NO: 4343; SEQ ID NO: 4344; SEQ ID NO: 4345; SEQ ID NO: 4346; SEQ ID NO: 4347; SEQ ID NO: 4348; SEQ ID NO: 4349; SEQ ID NO: 4350; SEQ ID NO: 4351; SEQ ID NO: 4352; SEQ ID NO: 4353; SEQ ID NO: 4354; SEQ ID NO: 4355; SEQ ID NO: 4356; SEQ ID NO: 4357; SEQ ID NO: 4358; SEQ ID NO: 4359; SEQ ID NO: 4360; SEQ ID NO: 4361; SEQ ID NO: 4362; SEQ ID NO: 4363; SEQ ID NO: 4364; SEQ ID NO: 4365; SEQ ID NO: 4366; SEQ ID NO: 4367; SEQ ID NO: 4368; SEQ ID NO: 4369; SEQ ID NO: 4370; SEQ ID NO: 4371; SEQ ID NO: 4372; SEQ ID NO: 4373; SEQ ID NO: 4374; SEQ ID NO: 4375; SEQ ID NO: 4376; SEQ ID NO: 4377; SEQ ID NO: 4378; SEQ ID NO: 4379; SEQ ID NO: 4380; SEQ ID NO: 4381; SEQ ID NO: 4382; SEQ ID NO: 4383; SEQ ID NO: 4384; SEQ ID NO: 4385; SEQ ID NO: 4386; SEQ ID NO: 4387; SEQ ID NO: 4388; and SEQ ID NO: 9622; SEQ ID NO: 9623; SEQ ID NO: 9624 and SEQ ID NO: 9625, or a complement therof.  
     [0087] In one embodiment, the  H. pylori  secreted polypeptide or a fragment thereof is an  H. pylori  periplasmic polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 4016; SEQ ID NO: 4017 and SEQ ID NO: 4018, or a complement thereof.  
     [0088] In another embodiment, the  H. pylori  secreted polypeptide or a fragment thereof is an  H. pylori  polypeptide or a fragment thereof involved in secretion and adhesion encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 4019; SEQ ID NO: 4020; SEQ ID NO: 4021; SEQ ID NO: 4022; SEQ ID NO: 4023; SEQ ID NO: 4024; SEQ ID NO: 4025; SEQ ID NO: 4026 and SEQ ID NO: 9622, or a complement thereof.  
     [0089] In another embodiment, the  H. pylori  secreted polypeptide or a fragment thereof is an  H. pylori  chaperone polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 4027; SEQ ID NO: 4028; SEQ ID NO: 4029 and SEQ ID NO: 4030, or a complement thereof.  
     [0090] Particularly preferred is an isolated nucleic acid having a nucleotide sequence encoding an  H. pylori  cellular polypeptide or a fragment thereof, the nucleic acid selected from the group consisting of SEQ ID NO: 4389; SEQ ID NO: 4390; SEQ ID NO: 4391; SEQ ID NO: 4392; SEQ ID NO: 4393; SEQ ID NO: 4394; SEQ ID NO: 4395; SEQ ID NO: 4396; SEQ ID NO: 4397; SEQ ID NO: 4398; SEQ ID NO: 4399; SEQ ID NO: 4400; SEQ ID NO: 4401; SEQ ID NO: 4402; SEQ ID NO: 4403; SEQ ID NO: 4404; SEQ ID NO: 4405; SEQ ID NO: 4406; SEQ ID NO: 4407; SEQ ID NO: 4408; SEQ ID NO: 4409; SEQ ID NO: 4410; SEQ ID NO: 4411; SEQ ID NO: 4412; SEQ ID NO: 4413; SEQ ID NO: 4414; SEQ ID NO: 4415; SEQ ID NO: 4416; SEQ ID NO: 4417; SEQ ID NO: 4418; SEQ ID NO: 4419; SEQ ID NO: 4420; SEQ ID NO: 4421; SEQ ID NO: 4422; SEQ ID NO: 4423; SEQ ID NO: 4424; SEQ ID NO: 4425; SEQ ID NO: 4426; SEQ ID NO: 4427; SEQ ID NO: 4428; SEQ ID NO: 4429; SEQ ID NO: 4430; SEQ ID NO: 4431; SEQ ID NO: 4432; SEQ ID NO: 4433; SEQ ID NO: 4434; SEQ ID NO: 4435; SEQ ID NO: 4436; SEQ ID NO: 4437; SEQ ID NO: 4438; SEQ ID NO: 4439; SEQ ID NO: 4440; SEQ ID NO: 4441; SEQ ID NO: 4442; SEQ ID NO: 4443; SEQ ID NO: 4444; SEQ ID NO: 4445; SEQ ID NO: 4446; SEQ ID NO: 4447; SEQ ID NO: 4448; SEQ ID NO: 4449; SEQ ID NO: 4450; SEQ ID NO: 4451; SEQ ID NO: 4452; SEQ ID NO: 4453; SEQ ID NO: 4454; SEQ ID NO: 4455; SEQ ID NO: 4456; SEQ ID NO: 4457; SEQ ID NO: 4458; SEQ ID NO: 4459; SEQ ID NO: 4460; SEQ ID NO: 4461; SEQ ID NO: 4462; SEQ ID NO: 4463; SEQ ID NO: 4464; SEQ ID NO: 4465; SEQ ID NO: 4466; SEQ ID NO: 4467; SEQ ID NO: 4468; SEQ ID NO: 4469; SEQ ID NO: 4470; SEQ ID NO: 4471; SEQ ID NO: 4472; SEQ ID NO: 4473; SEQ ID NO: 4474; SEQ ID NO: 4475; SEQ ID NO: 4476; SEQ ID NO: 4477; SEQ ID NO: 4478; SEQ ID NO: 4479; SEQ ID NO: 4480; SEQ ID NO: 4481; SEQ ID NO: 4482; SEQ ID NO: 4483; SEQ ID NO: 4484; SEQ ID NO: 4485; SEQ ID NO: 4486; SEQ ID NO: 4487; SEQ ID NO: 4488; SEQ ID NO: 4489; SEQ ID NO: 4490; SEQ ID NO: 4491; SEQ ID NO: 4492; SEQ ID NO: 4493; SEQ ID NO: 4494; SEQ ID NO: 4495; SEQ ID NO: 4496; SEQ ID NO: 4497; SEQ ID NO: 4498; SEQ ID NO: 4499; SEQ ID NO: 4500; SEQ ID NO: 4501; SEQ ID NO: 4502; SEQ ID NO: 4503; SEQ ID NO: 4504; SEQ ID NO: 4505; SEQ ID NO: 4506; SEQ ID NO: 4507; SEQ ID NO: 4508; SEQ ID NO: 4509; SEQ ID NO: 4510; SEQ ID NO: 4511; SEQ ID NO: 4512; SEQ ID NO: 4513; SEQ ID NO: 4514; SEQ ID NO: 4515; SEQ ID NO: 4516; SEQ ID NO: 4517; SEQ ID NO: 4518; SEQ ID NO: 4519; SEQ ID NO: 4520; SEQ ID NO: 4521; SEQ ID NO: 4522; SEQ ID NO: 4523; SEQ ID NO: 4524; SEQ ID NO: 4525; SEQ ID NO: 4526; SEQ ID NO: 4527; SEQ ID NO: 4528; SEQ ID NO: 4529; SEQ ID NO: 4530; SEQ ID NO: 4531; SEQ ID NO: 4532; SEQ ID NO: 4533; SEQ ID NO: 4534; SEQ ID NO: 4535; SEQ ID NO: 4536; SEQ ID NO: 4537; SEQ ID NO: 4538; SEQ ID NO: 4539; SEQ ID NO: 4540; SEQ ID NO: 4541; SEQ ID NO: 4542; SEQ ID NO: 4543; SEQ ID NO: 4544; SEQ ID NO: 4545; SEQ ID NO: 4546; SEQ ID NO: 4547; SEQ ID NO: 4548; SEQ ID NO: 4549; SEQ ID NO: 4550; SEQ ID NO: 4551; SEQ ID NO: 4552; SEQ ID NO: 4553; SEQ ID NO: 4554; SEQ ID NO: 4555; SEQ ID NO: 4556; SEQ ID NO: 4557; SEQ ID NO: 4558; SEQ ID NO: 4559; SEQ ID NO: 4560; SEQ ID NO: 4561; SEQ ID NO: 4562; SEQ ID NO: 4563; SEQ ID NO: 4564; SEQ ID NO: 4565; SEQ ID NO: 4566; SEQ ID NO: 4567; SEQ ID NO: 4568; SEQ ID NO: 4569; SEQ ID NO: 4570; SEQ ID NO: 4571; SEQ ID NO: 4572; SEQ ID NO: 4573; SEQ ID NO: 4574; SEQ ID NO: 4575; SEQ ID NO: 4576; SEQ ID NO: 4577; SEQ ID NO: 4578; SEQ ID NO: 4579; SEQ ID NO: 4580; SEQ ID NO: 4581; SEQ ID NO: 4582; SEQ ID NO: 4583; SEQ ID NO: 4584; SEQ ID NO: 4585; SEQ ID NO: 4586; SEQ ID NO: 4587; SEQ ID NO: 4588; SEQ ID NO: 4589; SEQ ID NO: 4590; SEQ ID NO: 4591; SEQ ID NO: 4592; SEQ ID NO: 4593; SEQ ID NO: 4594; SEQ ID NO: 4595; SEQ ID NO: 4596; SEQ ID NO: 4597; SEQ ID NO: 4598; SEQ ID NO: 4599; SEQ ID NO: 4600; SEQ ID NO: 4601; SEQ ID NO: 4602; SEQ ID NO: 4603; SEQ ID NO: 4604; SEQ ID NO: 4605; SEQ ID NO: 4606; SEQ ID NO: 4607; SEQ ID NO: 4608; SEQ ID NO: 4609; SEQ ID NO: 4610; SEQ ID NO: 4611; SEQ ID NO: 4612; SEQ ID NO: 4613; SEQ ID NO: 4614; SEQ ID NO: 4615; SEQ ID NO: 4616; SEQ ID NO: 4617; SEQ ID NO: 4618; SEQ ID NO: 4619; SEQ ID NO: 4620; SEQ ID NO: 4621; SEQ ID NO: 4622; SEQ ID NO: 4623; SEQ ID NO: 4624; SEQ ID NO: 4625; SEQ ID NO: 4626; SEQ ID NO: 4627; SEQ ID NO: 4628; SEQ ID NO: 4629; SEQ ID NO: 4630; SEQ ID NO: 4631; SEQ ID NO: 4632; SEQ ID NO: 4633; SEQ ID NO: 4634; SEQ ID NO: 4635; SEQ ID NO: 4636; SEQ ID NO: 4637; SEQ ID NO: 4638; SEQ ID NO: 4639; SEQ ID NO: 4640; SEQ ID NO: 4641; SEQ ID NO: 4642; SEQ ID NO: 4643; SEQ ID NO: 4644; SEQ ID NO: 4645; SEQ ID NO: 4646; SEQ ID NO: 4647; SEQ ID NO: 4648; SEQ ID NO: 4649; SEQ ID NO: 4650; SEQ ID NO: 4651; SEQ ID NO: 4652; SEQ ID NO: 4653; SEQ ID NO: 4654; SEQ ID NO: 4655; SEQ ID NO: 4656; SEQ ID NO: 4657; SEQ ID NO: 4658; SEQ ID NO: 4659; SEQ ID NO: 4660; SEQ ID NO: 4661; SEQ ID NO: 4662; SEQ ID NO: 4663; SEQ ID NO: 4664; SEQ ID NO: 4665; SEQ ID NO: 4666; SEQ ID NO: 4667; SEQ ID NO: 4668; SEQ ID NO: 4669; SEQ ID NO: 4670; SEQ ID NO: 4671; SEQ ID NO: 4672; SEQ ID NO: 4673; SEQ ID NO: 4674; SEQ ID NO: 4675; SEQ ID NO: 4676; SEQ ID NO: 4677; SEQ ID NO: 4678; SEQ ID NO: 4679; SEQ ID NO: 4680; SEQ ID NO: 4681; SEQ ID NO: 4682; SEQ ID NO: 4683; SEQ ID NO: 4684; SEQ ID NO: 4685; SEQ ID NO: 4686; SEQ ID NO: 4687; SEQ ID NO: 4688; SEQ ID NO: 4689; SEQ ID NO: 4690; SEQ ID NO: 4691; SEQ ID NO: 4692; SEQ ID NO: 4693; SEQ ID NO: 4694; SEQ ID NO: 4695; SEQ ID NO: 4696; SEQ ID NO: 4697; SEQ ID NO: 4698; SEQ ID NO: 4699; SEQ ID NO: 4700; SEQ ID NO: 4701; SEQ ID NO: 4702; SEQ ID NO: 4703; SEQ ID NO: 4704; SEQ ID NO: 4705; SEQ ID NO: 9626; SEQ ID NO: 9627; SEQ ID NO: 9628; SEQ ID NO: 9629; SEQ ID NO: 9630; SEQ ID NO: 9631; SEQ ID NO: 9632; SEQ ID NO: 9633; SEQ ID NO: 9634; SEQ ID NO: 9635 and SEQ ID NO: 9636, or a complement thereof.  
     [0091] Particularly preferred is an isolated nucleic acid having a nucleotide sequence encoding an  H. pylori  membrane associated polypeptide or a fragment thereof, the nucleic acid selected from the group consisting of SEQ ID NO: 4706; SEQ ID NO: 4707; SEQ ID NO: 4708; SEQ ID NO: 4709; SEQ ID NO: 4710; SEQ ID NO: 4711; SEQ ID NO: 4712; SEQ ID NO: 4713; SEQ ID NO: 4714; SEQ ID NO: 4715; SEQ ID NO: 4716; SEQ ID NO: 4717; SEQ ID NO: 4718; SEQ ID NO: 4719; SEQ ID NO: 4720; SEQ ID NO: 4721; SEQ ID NO: 4722; SEQ ID NO: 4723; SEQ ID NO: 4724; SEQ ID NO: 4725; SEQ ID NO: 4726; SEQ ID NO: 4727; SEQ ID NO: 4728; SEQ ID NO: 4729; SEQ ID NO: 4730; SEQ ID NO: 4731; SEQ ID NO: 4732; SEQ ID NO: 4733; SEQ ID NO: 4734; SEQ ID NO: 4735; SEQ ID NO: 4736; SEQ ID NO: 4737; SEQ ID NO: 4738; SEQ ID NO: 4739; SEQ ID NO: 4740; SEQ ID NO: 4741; SEQ ID NO: 4742; SEQ ID NO: 4743; SEQ ID NO: 4744; SEQ ID NO: 4745; SEQ ID NO: 4746; SEQ ID NO: 4747; SEQ ID NO: 4748; SEQ ID NO: 4749; SEQ ID NO: 4750; SEQ ID NO: 4751; SEQ ID NO: 4752; SEQ ID NO: 4753; SEQ ID NO: 4754; SEQ ID NO: 4755; SEQ ID NO: 4756; SEQ ID NO: 4757; SEQ ID NO: 4758; SEQ ID NO: 4759; SEQ ID NO: 4760; SEQ ID NO: 4761 and SEQ ID NO: 4762, or a complement thereof.  
     [0092] In another aspect, the invention features a probe having a nucleotide sequence consisting of at least 8 nucleotides of a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-SEQ ID NO: 4762 and SEQ ID NO: 9525-SEQ ID NO: 9636, or a complement thereof.  
     [0093] In another aspect, the invention features an isolated  H. pylori  polypeptide having an amino acid sequence at least about 60% homologous to an  H. pylori  polypeptide selected from the group consisting of SEQ ID NO: 4763-SEQ ID NO: 9524 and SEQ ID NO: 9637-SEQ ID NO: 9748.  
     [0094] In another aspect, the invention features an isolated  H. pylori  polypeptide which is encoded by a nucleic acid having a nucleotide sequence at least about 60% homologous to a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-SEQ ID NO:4762 and SEQ ID NO: 9525-SEQ ID NO: 9636. In one embodiment, the isolated  H. pylori  polypeptide is encoded by a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-SEQ ID NO: 4762 and SEQ ID NO: 9525-SEQ ID NO: 9636.  
     [0095] In another aspect, the invention features an isolated  H. pylori  polypeptide which is encoded by a nucleic acid which hybridizes under stringent hybridization conditions to a nucleic acid selected from the group consisting of SEQ ID NO: 1-SEQ ID NO: 4762 and SEQ ID NO: 9525-SEQ ID NO: 9636, or a complement thereof.  
     [0096] In another aspect, the invention features an isolated  H. pylori  polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO: 4763-SEQ ID NO: 9524 and SEQ ID NO: 9637-SEQ ID NO: 9748.  
     [0097] Particularly preferred is an isolated  H. pylori  cell envelope polypeptide or a fragment thereof, wherein the polypeptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 4763; SEQ ID NO: 4764; SEQ ID NO: 4765; SEQ ID NO: 4766; SEQ ID NO: 4767; SEQ ID NO: 4768; SEQ ID NO: 4769; SEQ ID NO: 4770; SEQ ID NO: 4771; SEQ ID NO: 4772; SEQ ID NO: 4773; SEQ ID NO: 4774; SEQ ID NO: 4775; SEQ ID NO: 4776; SEQ ID NO: 4777; SEQ ID NO: 4778; SEQ ID NO: 4779; SEQ ID NO: 4780; SEQ ID NO: 4781; SEQ ID NO: 4782; SEQ ID NO: 4783; SEQ ID NO: 4784; SEQ ID NO: 4785; SEQ ID NO: 4786; SEQ ID NO: 4787; SEQ ID NO: 4788; SEQ ID NO: 4789; SEQ ID NO: 4790; SEQ ID NO: 4791; SEQ ID NO: 4792; SEQ ID NO: 4793; SEQ ID NO: 4794; SEQ ID NO: 4795; SEQ ID NO: 4796; SEQ ID NO: 4797; SEQ ID NO: 4798; SEQ ID NO: 4799; SEQ ID NO: 4800; SEQ ID NO: 4801; SEQ ID NO: 4802; SEQ ID NO: 4803; SEQ ID NO: 4804; SEQ ID NO: 4805; SEQ ID NO: 4806; SEQ ID NO: 4807; SEQ ID NO: 4808; SEQ ID NO: 4809; SEQ ID NO: 4810; SEQ ID NO: 4811; SEQ ID NO: 4812; SEQ ID NO: 4813; SEQ ID NO: 4814; SEQ ID NO: 4815; SEQ ID NO: 4816; SEQ ID NO: 4817; SEQ ID NO: 4818; SEQ ID NO: 4819; SEQ ID NO: 4820; SEQ ID NO: 4821; SEQ ID NO: 4822; SEQ ID NO: 4823; SEQ ID NO: 4824; SEQ ID NO: 4825; SEQ ID NO: 4826; SEQ ID NO: 4827; SEQ ID NO: 4828; SEQ ID NO: 4829; SEQ ID NO: 4830; SEQ ID NO: 4831; SEQ ID NO: 4832; SEQ ID NO: 4833; SEQ ID NO: 4834; SEQ ID NO: 4835; SEQ ID NO: 4836; SEQ ID NO: 4837; SEQ ID NO: 4838; SEQ ID NO: 4839; SEQ ID NO: 4840; SEQ ID NO: 4841; SEQ ID NO: 4842; SEQ ID NO: 4843; SEQ ID NO: 4844; SEQ ID NO: 4845; SEQ ID NO: 4846; SEQ ID NO: 4847; SEQ ID NO: 4848; SEQ ID NO: 4849; SEQ ID NO: 4850; SEQ ID NO: 4851; SEQ ID NO: 4852; SEQ ID NO: 4853; SEQ ID NO: 4854; SEQ ID NO: 4855; SEQ ID NO: 4856; SEQ ID NO: 4857; SEQ ID NO: 4858; SEQ ID NO: 4859; SEQ ID NO: 4860; SEQ ID NO: 4861; SEQ ID NO: 4862; SEQ ID NO: 4863; SEQ ID NO: 4864; SEQ ID NO: 4865; SEQ ID NO: 4866; SEQ ID NO: 4867; SEQ ID NO: 4868; SEQ ID NO: 4869; SEQ ID NO: 4870; SEQ ID NO: 4871; SEQ ID NO: 4872; SEQ ID NO: 4873; SEQ ID NO: 4874; SEQ ID NO: 4875; SEQ ID NO: 4876; SEQ ID NO: 4877; SEQ ID NO: 4878; SEQ ID NO: 4879; SEQ ID NO: 4880; SEQ ID NO: 4881; SEQ ID NO: 4882; SEQ ID NO: 4883; SEQ ID NO: 4884; SEQ ID NO: 4885; SEQ ID NO: 4886; SEQ ID NO: 4887; SEQ ID NO: 4888; SEQ ID NO: 4889; SEQ ID NO: 4890; SEQ ID NO: 4891; SEQ ID NO: 4892; SEQ ID NO: 4893; SEQ ID NO: 4894; SEQ ID NO: 4895; SEQ ID NO: 4896; SEQ ID NO: 4897; SEQ ID NO: 4898; SEQ ID NO: 4899; SEQ ID NO: 4900; SEQ ID NO: 4901; SEQ ID NO: 4902; SEQ ID NO: 4903; SEQ ID NO: 4904; SEQ ID NO: 4905; SEQ ID NO: 4906; SEQ ID NO: 4907; SEQ ID NO: 4908; SEQ ID NO: 4909; SEQ ID NO: 4910; SEQ ID NO: 4911; SEQ ID NO: 4912; SEQ ID NO: 4913; SEQ ID NO: 4914; SEQ ID NO: 4915; SEQ ID NO: 4916; SEQ ID NO: 4917; SEQ ID NO: 4918; SEQ ID NO: 4919; SEQ ID NO: 4920; SEQ ID NO: 4921; SEQ ID NO: 4922; SEQ ID NO: 4923; SEQ ID NO: 4924; SEQ ID NO: 4925; SEQ ID NO: 4926; SEQ ID NO: 4927; SEQ ID NO: 4928; SEQ ID NO: 4929; SEQ ID NO: 4930; SEQ ID NO: 4931; SEQ ID NO: 4932; SEQ ID NO: 4933; SEQ ID NO: 4934; SEQ ID NO: 4935; SEQ ID NO: 4936; SEQ ID NO: 4937; SEQ ID NO: 4938; SEQ ID NO: 4939; SEQ ID NO: 4940; SEQ ID NO: 4941; SEQ ID NO: 4942; SEQ ID NO: 4943; SEQ ID NO: 4944; SEQ ID NO: 4945; SEQ ID NO: 4946; SEQ ID NO: 4947; SEQ ID NO: 4948; SEQ ID NO: 4949; SEQ ID NO: 4950; SEQ ID NO: 4951; SEQ ID NO: 4952; SEQ ID NO: 4953; SEQ ID NO: 4954; SEQ ID NO: 4955; SEQ ID NO: 4956; SEQ ID NO: 4957; SEQ ID NO: 4958; SEQ ID NO: 4959; SEQ ID NO: 4960; SEQ ID NO: 4961; SEQ ID NO: 4962; SEQ ID NO: 4963; SEQ ID NO: 4964; SEQ ID NO: 4965; SEQ ID NO: 4966; SEQ ID NO: 4967; SEQ ID NO: 4968; SEQ ID NO: 4969; SEQ ID NO: 4970; SEQ ID NO: 4971; SEQ ID NO: 4972; SEQ ID NO: 4973; SEQ ID NO: 4974; SEQ ID NO: 4975; SEQ ID NO: 4976; SEQ ID NO: 4977; SEQ ID NO: 4978; SEQ ID NO: 4979; SEQ ID NO: 4980; SEQ ID NO: 4981; SEQ ID NO: 4982; SEQ ID NO: 4983; SEQ ID NO: 4984; SEQ ID NO: 4985; SEQ ID NO: 4986; SEQ ID NO: 4987; SEQ ID NO: 4988; SEQ ID NO: 4989; SEQ ID NO: 4990; SEQ ID NO: 4991; SEQ ID NO: 4992; SEQ ID NO: 4993; SEQ ID NO: 4994; SEQ ID NO: 4995; SEQ ID NO: 4996; SEQ ID NO: 4997; SEQ ID NO: 4998; SEQ ID NO: 4999; SEQ ID NO: 5000; SEQ ID NO: 5001; SEQ ID NO: 5002; SEQ ID NO: 5003; SEQ ID NO: 5004; SEQ ID NO: 5005; SEQ ID NO: 5006; SEQ ID NO: 5007; SEQ ID NO: 5008; SEQ ID NO: 5009; SEQ ID NO: 5010; SEQ ID NO: 5011; SEQ ID NO: 5012; SEQ ID NO: 5013; SEQ ID NO: 5014; SEQ ID NO: 5015; SEQ ID NO: 5016; SEQ ID NO: 5017; SEQ ID NO: 5018; SEQ ID NO: 5019; SEQ ID NO: 5020; SEQ ID NO: 5021; SEQ ID NO: 5022; SEQ ID NO: 5023; SEQ ID NO: 5024; SEQ ID NO: 5025; SEQ ID NO: 5026; SEQ ID NO: 5027; SEQ ID NO: 5028; SEQ ID NO: 5029; SEQ ID NO: 5030; SEQ ID NO: 5031; SEQ ID NO: 5032; SEQ ID NO: 5033; SEQ ID NO: 5034; SEQ ID NO: 5035; SEQ ID NO: 5036; SEQ ID NO: 5037; SEQ ID NO: 5038; SEQ ID NO: 5039; SEQ ID NO: 5040; SEQ ID NO: 5041; SEQ ID NO: 5042; SEQ ID NO: 5043; SEQ ID NO: 5044; SEQ ID NO: 5045; SEQ ID NO: 5046; SEQ ID NO: 5047; SEQ ID NO: 5048; SEQ ID NO: 5049; SEQ ID NO: 5050; SEQ ID NO: 5051; SEQ ID NO: 5052; SEQ ID NO: 5053; SEQ ID NO: 5054; SEQ ID NO: 5055; SEQ ID NO: 5056; SEQ ID NO: 5057; SEQ ID NO: 5058; SEQ ID NO: 5059; SEQ ID NO: 5060; SEQ ID NO: 5061; SEQ ID NO: 5062; SEQ ID NO: 5063; SEQ ID NO: 5064; SEQ ID NO: 5065; SEQ ID NO: 5066; SEQ ID NO: 5067; SEQ ID NO: 5068; SEQ ID NO: 5069; SEQ ID NO: 5070; SEQ ID NO: 5071; SEQ ID NO: 5072; SEQ ID NO: 5073; SEQ ID NO: 5074; SEQ ID NO: 5075; SEQ ID NO: 5076; SEQ ID NO: 5077; SEQ ID NO: 5078; SEQ ID NO: 5079; SEQ ID NO: 5080; SEQ ID NO: 5081; SEQ ID NO: 5082; SEQ ID NO: 5083; SEQ ID NO: 5084; SEQ ID NO: 5085; SEQ ID NO: 5086; SEQ ID NO: 5087; SEQ ID NO: 5088; SEQ ID NO: 5089; SEQ ID NO: 5090; SEQ ID NO: 5091; SEQ ID NO: 5092; SEQ ID NO: 5093; SEQ ID NO: 5094; SEQ ID NO: 5095; SEQ ID NO: 5096; SEQ ID NO: 5097; SEQ ID NO: 5098; SEQ ID NO: 5099; SEQ ID NO: 5100; SEQ ID NO: 5101; SEQ ID NO: 5102, SEQ ID NO: 5103; SEQ ID NO: 5104; SEQ ID NO: 5105; SEQ ID NO: 5106; SEQ ID NO: 5107; SEQ ID NO: 5108; SEQ ID NO:5109; SEQ ID NO: 5110; SEQ ID NO:5111; SEQ ID NO:5112; SEQ ID NO: 5113; SEQ ID NO: 5114; SEQ ID NO: 5115; SEQ ID NO: 5116; SEQ ID NO: 5117; SEQ ID NO: 5118; SEQ ID NO: 5119; SEQ ID NO: 5120; SEQ ID NO: 5121; SEQ ID NO: 5122; SEQ ID NO: 5123; SEQ ID NO: 5124; SEQ ID NO: 5125; SEQ ID NO: 5126; SEQ ID NO: 5127; SEQ ID NO: 5128; SEQ ID NO: 5129; SEQ ID NO: 5130; SEQ ID NO: 5131; SEQ ID NO: 5132; SEQ ID NO: 5133; SEQ ID NO: 5134; SEQ ID NO: 5135; SEQ ID NO: 5136; SEQ ID NO: 5137; SEQ ID NO: 5138; SEQ ID NO: 5139; SEQ ID NO: 5140; SEQ ID NO: 5141; SEQ ID NO: 5142; SEQ ID NO: 5143; SEQ ID NO: 5144; SEQ ID NO: 5145; SEQ ID NO: 5146; SEQ ID NO: 5147; SEQ ID NO: 5148; SEQ ID NO: 5149; SEQ ID NO: 5150; SEQ ID NO: 5151; SEQ ID NO: 5152; SEQ ID NO: 5153; SEQ ID NO: 5154; SEQ ID NO: 5155; SEQ ID NO: 5156; SEQ ID NO: 5157; SEQ ID NO: 5158; SEQ ID NO: 5159; SEQ ID NO: 5160; SEQ ID NO: 5161; SEQ ID NO: 5162; SEQ ID NO: 5163; SEQ ID NO: 5164; SEQ ID NO: 5165; SEQ ID NO: 5166; SEQ ID NO: 5167; SEQ ID NO: 5168; SEQ ID NO: 5169; SEQ ID NO: 5170; SEQ ID NO: 5171; SEQ ID NO: 5172; SEQ ID NO: 5173; SEQ ID NO: 5174; SEQ ID NO: 5175; SEQ ID NO: 5176; SEQ ID NO: 5177; SEQ ID NO: 5178; SEQ ID NO: 5179; SEQ ID NO: 5180; SEQ ID NO: 5181; SEQ ID NO: 5182; SEQ ID NO: 5183; SEQ ID NO: 5184; SEQ ID NO: 5185; SEQ ID NO: 5186; SEQ ID NO: 5187; SEQ ID NO: 5188; SEQ ID NO: 5189; SEQ ID NO: 5190; SEQ ID NO: 5191; SEQ ID NO: 5192; SEQ ID NO: 5193; SEQ ID NO: 5194; SEQ ID NO: 5195; SEQ ID NO: 5196; SEQ ID NO: 5197; SEQ ID NO: 5198; SEQ ID NO: 5199; SEQ ID NO: 5200; SEQ ID NO: 5201; SEQ ID NO: 5202; SEQ ID NO: 5203; SEQ ID NO: 5204; SEQ ID NO: 5205; SEQ ID NO: 5206; SEQ ID NO: 5207; SEQ ID NO: 5208; SEQ ID NO: 5209; SEQ ID NO: 5210; SEQ ID NO: 5211; SEQ ID NO: 5212; SEQ ID NO: 5213; SEQ ID NO: 5214; SEQ ID NO: 5215; SEQ ID NO: 5216; SEQ ID NO: 5217; SEQ ID NO: 5218; SEQ ID NO: 5219; SEQ ID NO: 5220; SEQ ID NO: 5221; SEQ ID NO: 5222; SEQ ID NO: 5223; SEQ ID NO: 5224; SEQ ID NO: 5225; SEQ ID NO: 5226; SEQ ID NO: 5227; SEQ ID NO: 5228; SEQ ID NO: 5229; SEQ ID NO: 5230; SEQ ID NO: 5231; SEQ ID NO: 5232; SEQ ID NO: 5233; SEQ ID NO: 5234; SEQ ID NO: 5235; SEQ ID NO: 5236; SEQ ID NO: 5237; SEQ ID NO: 5238; SEQ ID NO: 5239; SEQ ID NO: 5240; SEQ ID NO: 5241; SEQ ID NO: 5242; SEQ ID NO: 5243; SEQ ID NO: 5244; SEQ ID NO: 5245; SEQ ID NO: 5246; SEQ ID NO: 5247; SEQ ID NO: 5248; SEQ ID NO: 5249; SEQ ID NO: 5250; SEQ ID NO: 5251; SEQ ID NO: 5252; SEQ ID NO: 5253; SEQ ID NO: 5254; SEQ ID NO: 5255; SEQ ID NO: 5256; SEQ ID NO: 52.57; SEQ ID NO: 5258; SEQ ID NO: 5259; SEQ ID NO: 5260; SEQ ID NO: 5261; SEQ ID NO: 5262; SEQ ID NO: 5263; SEQ ID NO: 5264; SEQ ID NO: 5265; SEQ ID NO: 5266; SEQ ID NO: 5267; SEQ ID NO: 5268; SEQ ID NO: 5269; SEQ ID NO: 5270; SEQ ID NO: 5271; SEQ ID NO: 5272; SEQ ID NO: 5273; SEQ ID NO: 5274; SEQ ID NO: 5275; SEQ ID NO: 5276; SEQ ID NO: 5277; SEQ ID NO: 5278; SEQ ID NO: 5279; SEQ ID NO: 5280; SEQ ID NO: 5281; SEQ ID NO: 5282; SEQ ID NO: 5283; SEQ ID NO: 5284; SEQ ID NO: 5285; SEQ ID NO: 5286; SEQ ID NO: 5287; SEQ ID NO: 5288; SEQ ID NO: 5289; SEQ ID NO: 5290; SEQ ID NO: 5291; SEQ ID NO: 5292; SEQ ID NO: 5293; SEQ ID NO: 5294; SEQ ID NO: 5295; SEQ ID NO: 5296; SEQ ID NO: 5297; SEQ ID NO: 5298; SEQ ID NO: 5299; SEQ ID NO: 5300; SEQ ID NO: 5301; SEQ ID NO: 5302; SEQ ID NO: 5303; SEQ ID NO: 5304; SEQ ID NO: 5305; SEQ ID NO: 5306; SEQ ID NO: 5307; SEQ ID NO: 5308; SEQ ID NO: 5309; SEQ ID NO: 5310; SEQ ID NO: 5311; SEQ ID NO: 5312; SEQ ID NO: 5313; SEQ ID NO: 5314; SEQ ID NO: 5315; SEQ ID NO: 5316; SEQ ID NO: 5317; SEQ ID NO: 5318; SEQ ID NO: 5319; SEQ ID NO: 5320; SEQ ID NO: 5321; SEQ ID NO: 5322; SEQ ID NO: 5323; SEQ ID NO: 5324; SEQ ID NO: 5325; SEQ ID NO: 5326; SEQ ID NO: 5327; SEQ ID NO: 5328; SEQ ID NO: 5329; SEQ ID NO: 5330; SEQ ID NO: 5331; SEQ ID NO: 5332; SEQ ID NO: 5333; SEQ ID NO: 5334; SEQ ID NO: 5335; SEQ ID NO: 5336; SEQ ID NO: 5337; SEQ ID NO: 5338; SEQ ID NO: 5339; SEQ ID NO: 5340; SEQ ID NO: 5341; SEQ ID NO: 5342; SEQ ID NO: 5343; SEQ ID NO: 5344; SEQ ID NO: 5345; SEQ ID NO: 5346; SEQ ID NO: 5347; SEQ ID NO: 5348; SEQ ID NO: 5349; SEQ ID NO: 5350; SEQ ID NO: 5351; SEQ ID NO: 5352; SEQ ID NO: 5353; SEQ ID NO: 5354; SEQ ID NO: 5355; SEQ ID NO: 5356; SEQ ID NO: 5357; SEQ ID NO: 5358; SEQ ID NO: 5359; SEQ ID NO: 5360; SEQ ID NO: 5361; SEQ ID NO: 5362; SEQ ID NO: 5363; SEQ ID NO: 5364; SEQ ID NO: 5365; SEQ ID NO: 5366; SEQ ID NO: 5367; SEQ ID NO: 5368; SEQ ID NO: 5369; SEQ ID NO: 5370; SEQ ID NO: 5371; SEQ ID NO: 5372; SEQ ID NO: 5373; SEQ ID NO: 5374; SEQ ID NO: 5375; SEQ ID NO: 5376; SEQ ID NO: 5377; SEQ ID NO: 5378; SEQ ID NO: 5379; SEQ ID NO: 5380; SEQ ID NO: 5381; SEQ ID NO: 5382; SEQ ID NO: 5383; SEQ ID NO: 5384; SEQ ID NO: 5385; SEQ ID NO: 5386; SEQ ID NO: 5387; SEQ ID NO: 5388; SEQ ID NO: 5389; SEQ ID NO: 5390; SEQ ID NO: 5391; SEQ ID NO: 5392; SEQ ID NO: 5393; SEQ ID NO: 5394; SEQ ID NO: 5395; SEQ ID NO: 5396; SEQ ID NO: 5397; SEQ ID NO: 5398; SEQ ID NO: 5399; SEQ ID NO: 5400; SEQ ID NO: 5401; SEQ ID NO: 5402; SEQ ID NO: 5403; SEQ ID NO: 5404; SEQ ID NO: 5405; SEQ ID NO: 5406; SEQ ID NO: 5407; SEQ ID NO: 5408; SEQ ID NO: 5409; SEQ ID NO: 5410; SEQ ID NO: 5411; SEQ ID NO: 5412; SEQ ID NO: 5413; SEQ ID NO: 5414; SEQ ID NO: 5415; SEQ ID NO: 5416; SEQ ID NO: 5417; SEQ ID NO: 5418; SEQ ID NO: 5419; SEQ ID NO: 5420; SEQ ID NO: 5421; SEQ ID NO: 5422; SEQ ID NO: 5423; SEQ ID NO: 5424; SEQ ID NO: 5425; SEQ ID NO: 5426; SEQ ID NO: 5427; SEQ ID NO: 5428; SEQ ID NO: 5429; SEQ ID NO: 5430; SEQ ID NO: 5431; SEQ ID NO: 5432; SEQ ID NO: 5433; SEQ ID NO: 5434; SEQ ID NO: 5435; SEQ ID NO: 5436; SEQ ID NO: 5437; SEQ ID NO: 5438; SEQ ID NO: 5439; SEQ ID NO: 5440; SEQ ID NO: 5441; SEQ ID NO: 5442; SEQ ID NO: 5443; SEQ ID NO: 5444; SEQ ID NO: 5445; SEQ ID NO: 5446; SEQ ID NO: 5447; SEQ ID NO: 5448; SEQ ID NO: 5449; SEQ ID NO: 5450; SEQ ID NO: 5451; SEQ ID NO: 5452; SEQ ID NO: 5453; SEQ ID NO: 5454; SEQ ID NO: 5455; SEQ ID NO: 5456; SEQ ID NO: 5457; SEQ ID NO: 5458; SEQ ID NO: 5459; SEQ ID NO: 5460; SEQ ID NO: 5461; SEQ ID NO: 5462; SEQ ID NO: 5463; SEQ ID NO: 5464; SEQ ID NO: 5465; SEQ ID NO: 5466; SEQ ID NO: 5467; SEQ ID NO: 5468; SEQ ID NO: 5469; SEQ ID NO: 5470; SEQ ID NO: 5471; SEQ ID NO: 5472; SEQ ID NO: 5473; SEQ ID NO: 5474; SEQ ID NO: 5475; SEQ ID NO: 5476; SEQ ID NO: 5477; SEQ ID NO: 5478; SEQ ID NO: 5479; SEQ ID NO: 5480; SEQ ID NO: 5481; SEQ ID NO: 5482; SEQ ID NO: 5483; SEQ ID NO: 5484; SEQ ID NO: 5485; SEQ ID NO: 5486; SEQ ID NO: 5487; SEQ ID NO: 5488; SEQ ID NO: 5489; SEQ ID NO: 5490; SEQ ID NO: 5491; SEQ ID NO: 5492; SEQ ID NO: 5493; SEQ ID NO: 5494; SEQ ID NO: 5495; SEQ ID NO: 5496; SEQ ID NO: 5497; SEQ ID NO: 5498; SEQ ID NO: 5499; SEQ ID NO: 5500; SEQ ID NO: 5501; SEQ ID NO: 5502; SEQ ID NO: 5503; SEQ ID NO: 5504; SEQ ID NO: 5505; SEQ ID NO: 5506; SEQ ID NO: 5507; SEQ ID NO: 5508; SEQ ID NO: 5509; SEQ ID NO: 5510; SEQ ID NO: 5511; SEQ ID NO: 5512; SEQ ID NO: 5513; SEQ ID NO: 5514; SEQ ID NO: 5515; SEQ ID NO: 5516; SEQ ID NO: 5517; SEQ ID NO: 5518; SEQ ID NO: 5519; SEQ ID NO: 5520; SEQ ID NO: 5521; SEQ ID NO: 5522; SEQ ID NO: 5523; SEQ ID NO: 5524; SEQ ID NO: 5525; SEQ ID NO: 5526; SEQ ID NO: 5527; SEQ ID NO: 5528; SEQ ID NO: 5529; SEQ ID NO: 5530; SEQ ID NO: 5531; SEQ ID NO: 5532; SEQ ID NO: 5533; SEQ ID NO: 5534; SEQ ID NO: 5535; SEQ ID NO: 5536; SEQ ID NO: 5537; SEQ ID NO: 5538; SEQ ID NO: 5539; SEQ ID NO: 5540; SEQ ID NO: 5541; SEQ ID NO: 5542; SEQ ID NO: 5543; SEQ ID NO: 5544; SEQ ID NO: 5545; SEQ ID NO: 5546; SEQ ID NO: 5547; SEQ ID NO: 5548; SEQ ID NO: 5549; SEQ ID NO: 5550; SEQ ID NO: 5551; SEQ ID NO: 5552; SEQ ID NO: 5553; SEQ ID NO: 5554; SEQ ID NO: 5555; SEQ ID NO: 5556; SEQ ID NO: 5557; SEQ ID NO: 5558; SEQ ID NO: 5559; SEQ ID NO: 5560; SEQ ID NO: 5561; SEQ ID NO: 5562; SEQ ID NO: 5563; SEQ ID NO: 5564; SEQ ID NO: 5565; SEQ ID NO: 5566; SEQ ID NO: 5567; SEQ ID NO: 5568; SEQ ID NO: 5569; SEQ ID NO: 5570; SEQ ID NO: 5571; SEQ ID NO: 5572; SEQ ID NO: 5573; SEQ ID NO: 5574; SEQ ID NO: 5575; SEQ ID NO: 5576; SEQ ID NO: 5577; SEQ ID NO: 5578; SEQ ID NO: 5579; SEQ ID NO: 5580; SEQ ID NO: 5581; SEQ ID NO: 5582; SEQ ID NO: 5583; SEQ ID NO: 5584; SEQ ID NO: 5585; SEQ ID NO: 5586; SEQ ID NO: 5587; SEQ ID NO: 5588; SEQ ID NO: 5589; SEQ ID NO: 5590; SEQ ID NO: 5591; SEQ ID NO: 5592; SEQ ID NO: 5593; SEQ ID NO: 5594; SEQ ID NO: 5595; SEQ ID NO: 5596; SEQ ID NO: 5597; S-Q ID NO: 5598; SEQ ID NO: 5599; SEQ ID NO: 5600; SEQ ID NO: 5601; SEQ ID NO: 5602; SEQ ID NO: 5603; SEQ ID NO: 5604; SEQ ID NO: 5605; SEQ ID NO: 5606; SEQ ID NO: 5607; SEQ ID NO: 5608; SEQ ID NO: 5609; SEQ ID NO: 5610; SEQ ID NO: 5611; SEQ ID NO: 5612; SEQ ID NO: 5613; SEQ ID NO: 5614; SEQ ID NO: 5615; SEQ ID NO: 5616; SEQ ID NO: 5617; SEQ ID NO: 5618; SEQ ID NO: 5619; SEQ ID NO: 5620; SEQ ID NO: 5621; SEQ ID NO: 5622; SEQ ID NO: 5623; SEQ ID NO: 5624; SEQ ID NO: 5625; SEQ ID NO: 5626; SEQ ID NO: 5627; SEQ ID NO: 5628; SEQ ID NO: 5629; SEQ ID NO: 5630; SEQ ID NO: 5631; SEQ ID NO: 5632; SEQ ID NO: 5633; SEQ ID NO: 5634; SEQ ID NO: 5635; SEQ ID NO: 5636; SEQ ID NO: 5637; SEQ ID NO: 5638; SEQ ID NO: 5639; SEQ ID NO: 5640; SEQ ID NO: 5641; SEQ ID NO: 5642; SEQ ID NO: 5643; SEQ ID NO: 5644; SEQ ID NO: 5645; SEQ ID NO: 5646; SEQ ID NO: 5647; SEQ ID NO: 5648; SEQ ID NO: 5649; SEQ ID NO: 5650; SEQ ID NO: 5651; SEQ ID NO: 5652; SEQ ID NO: 5653; SEQ ID NO: 5654; SEQ ID NO: 5655; SEQ ID NO: 5656; SEQ ID NO: 5657; SEQ ID NO: 5658; SEQ ID NO: 5659; SEQ ID NO: 5660; SEQ ID NO: 5661; SEQ ID NO: 5662; SEQ ID NO: 5663; SEQ ID NO: 5664; SEQ ID NO: 5665; SEQ ID NO: 5666; SEQ ID NO: 5667; SEQ ID NO: 5668; SEQ ID NO: 5669; SEQ ID NO: 5670; SEQ ID NO: 5671; SEQ ID NO: 5672; SEQ ID NO: 5673; SEQ ID NO: 5674; SEQ ID NO: 5675; SEQ ID NO: 5676; SEQ ID NO: 5677; SEQ ID NO: 5678; SEQ ID NO: 5679; SEQ ID NO: 5680; SEQ ID NO: 5681; SEQ ID NO: 5682; SEQ ID NO: 5683; SEQ ID NO: 5684; SEQ ID NO: 5685; SEQ ID NO: 5686; SEQ ID NO: 5687; SEQ ID NO: 5688; SEQ ID NO: 5689; SEQ ID NO: 5690; SEQ ID NO: 5691; SEQ ID NO: 5692; SEQ ID NO: 5693; SEQ ID NO: 5694; SEQ ID NO: 5695; SEQ ID NO: 5696; SEQ ID NO: 5697; SEQ ID NO: 5698; SEQ ID NO: 5699; SEQ ID NO: 5700; SEQ ID NO: 5701; SEQ ID NO: 5702; SEQ ID NO: 5703; SEQ ID NO: 5704; SEQ ID NO: 5705; SEQ ID NO: 5706; SEQ ID NO: 5707; SEQ ID NO: 5708; SEQ ID NO: 5709; SEQ ID NO: 5710; SEQ ID NO: 5711; SEQ ID NO: 5712; SEQ ID NO: 5713; SEQ ID NO: 5714; SEQ ID NO: 5715; SEQ ID NO: 5716; SEQ ID NO: 5717; SEQ ID NO: 5718; SEQ ID NO: 5719; SEQ ID NO:5720; SEQ ID NO:5721; SEQ ID NO:5722; SEQ ID NO:5723; SEQ ID NO: 5724; SEQ ID NO: 5725; SEQ ID NO: 5726; SEQ ID NO: 5727; SEQ ID NO: 5728; SEQ ID NO: 5729; SEQ ID NO: 5730; SEQ ID NO: 5731; SEQ ID NO: 5732; SEQ ID NO: 5733; SEQ ID NO: 5734; SEQ ID NO: 5735; SEQ ID NO: 5736; SEQ ID NO: 5737; SEQ ID NO: 5738; SEQ ID NO: 5739; SEQ ID NO: 5740; SEQ ID NO: 5741; SEQ ID NO: 5742; SEQ ID NO: 5743; SEQ ID NO: 5744; SEQ ID NO: 5745; SEQ ID NO: 5746; SEQ ID NO: 5747; SEQ ID NO: 5748; SEQ ID NO: 5749; SEQ ID NO: 5750; SEQ ID NO: 5751; SEQ ID NO: 5752; SEQ ID NO: 5753; SEQ ID NO: 5754; SEQ ID NO: 5755; SEQ ID NO: 5756; SEQ ID NO: 5757; SEQ ID NO: 5758; SEQ ID NO: 5759; SEQ ID NO: 5760; SEQ ID NO: 5761; SEQ ID NO: 5762; SEQ ID NO: 5763; SEQ ID NO: 5764; SEQ ID NO: 5765; SEQ ID NO: 5766; SEQ ID NO: 5767; SEQ ID NO: 5768; SEQ ID NO: 5769; SEQ ID NO: 5770; SEQ ID NO: 5771; SEQ ID NO: 5772; SEQ ID NO: 5773; SEQ ID NO: 5774; SEQ ID NO: 5775; SEQ ID NO: 5776; SEQ ID NO: 5777; SEQ ID NO: 5778; SEQ ID NO: 5779; SEQ ID NO: 5780; SEQ ID NO: 5781; SEQ ID NO: 5782; SEQ ID NO: 5783; SEQ ID NO: 5784; SEQ ID NO: 5785; SEQ ID NO: 5786; SEQ ID NO: 5787; SEQ ID NO: 5788; SEQ ID NO: 5789; SEQ ID NO: 5790; SEQ ID NO: 5791; SEQ ID NO: 5792; SEQ ID NO: 5793; SEQ ID NO: 5794; SEQ ID NO: 5795; SEQ ID NO: 5796; SEQ ID NO: 5797; SEQ ID NO: 5798; SEQ ID NO: 5799; SEQ ID NO: 5800; SEQ ID NO: 5801; SEQ ID NO: 5802; SEQ ID NO: 5803; SEQ ID NO: 5804; SEQ ID NO: 5805; SEQ ID NO: 5806; SEQ ID NO: 5807; SEQ ID NO: 5808; SEQ ID NO: 5809; SEQ ID NO: 5810; SEQ ID NO: 5811; SEQ ID NO: 5812; SEQ ID NO: 5813; SEQ ID NO: 5814; SEQ ID NO: 5815; SEQ ID NO: 5816; SEQ ID NO: 5817; SEQ ID NO: 5818; SEQ ID NO: 5819; SEQ ID NO: 5820; SEQ ID NO: 5821; SEQ ID NO: 5822; SEQ ID NO: 5823; SEQ ID NO: 5824; SEQ ID NO: 5825; SEQ ID NO: 5826; SEQ ID NO: 5827; SEQ ID NO: 5828; SEQ ID NO: 5829; SEQ ID NO: 5830; SEQ ID NO: 5831; SEQ ID NO: 5832; SEQ ID NO: 5833; SEQ ID NO: 5834; SEQ ID NO: 5835; SEQ ID NO: 5836; SEQ ID NO: 5837; SEQ ID NO: 5838; SEQ ID NO: 5839; SEQ ID NO: 5840; SEQ ID NO: 5841; SEQ ID NO: 5842; SEQ ID NO: 5843; SEQ ID NO: 5844; SEQ ID NO: 5845; SEQ ID NO: 5846; SEQ ID NO: 5847; SEQ ID NO: 5848; SEQ ID NO: 5849; SEQ ID NO: 5850; SEQ ID NO: 5851; SEQ ID NO: 5852; SEQ ID NO: 5853; SEQ ID NO: 5854; SEQ ID NO: 5855; SEQ ID NO: 5856; SEQ ID NO: 5857; SEQ ID NO: 5858; SEQ ID NO: 5859; SEQ ID NO: 5860; SEQ ID NO: 5861; SEQ ID NO: 5862; SEQ ID NO: 5863; SEQ ID NO: 5864; SEQ ID NO: 5865; SEQ ID NO: 5866; SEQ ID NO: 5867; SEQ ID NO: 5868; SEQ ID NO: 5869; SEQ ID NO: 5870; SEQ ID NO: 5871; SEQ ID NO: 5872; SEQ ID NO: 5873; SEQ ID NO: 5874; SEQ ID NO: 5875; SEQ ID NO: 5876; SEQ ID NO: 5877; SEQ ID NO: 5878; SEQ ID NO: 5879; SEQ ID NO: 5880; SEQ ID NO: 5881; SEQ ID NO: 5882; SEQ ID NO: 5883; SEQ ID NO: 5884; SEQ ID NO: 5885; SEQ ID NO: 5886; SEQ ID NO: 5887; SEQ ID NO: 5888; SEQ ID NO: 5889; SEQ ID NO: 5890; SEQ ID NO: 5891; SEQ ID NO: 5892; SEQ ID NO: 5893; SEQ ID NO: 5894; SEQ ID NO: 5895; SEQ ID NO: 5896; SEQ ID NO: 5897; SEQ ID NO: 5898; SEQ ID NO: 5899; SEQ ID NO: 5900; SEQ ID NO: 5901; SEQ ID NO: 5902; SEQ ID NO: 5903; SEQ ID NO: 5904; SEQ ID NO: 5905; SEQ ID NO: 5906; SEQ ID NO: 5907; SEQ ID NO: 5908; SEQ ID NO: 5909; SEQ ID NO: 5910; SEQ ID NO: 5911; SEQ ID NO: 5912; SEQ ID NO: 5913; SEQ ID NO: 5914; SEQ ID NO: 5915; SEQ ID NO: 5916; SEQ ID NO: 5917; SEQ ID NO: 5918; SEQ ID NO: 5919; SEQ ID NO: 5920; SEQ ID NO: 5921; SEQ ID NO: 5922; SEQ ID NO: 5923; SEQ ID NO: 5924; SEQ ID NO: 5925; SEQ ID NO: 5926; SEQ ID NO: 5927; SEQ ID NO: 5928; SEQ ID NO: 5929; SEQ ID NO: 5930; SEQ ID NO: 5931; SEQ ID NO: 5932; SEQ ID NO: 5933; SEQ ID NO: 5934; SEQ ID NO: 5935; SEQ ID NO: 5936; SEQ ID NO: 5937; SEQ ID NO: 5938; SEQ ID NO: 5939; SEQ ID NO: 5940; SEQ ID NO: 5941; SEQ ID NO: 5942; SEQ ID NO: 5943; SEQ ID NO: 5944; SEQ ID NO: 5945; SEQ ID NO: 5946; SEQ ID NO: 5947; SEQ ID NO: 5948; SEQ ID NO: 5949; SEQ ID NO: 5950; SEQ ID NO: 5951; SEQ ID NO: 5952; SEQ ID NO: 5953; SEQ ID NO: 5954; SEQ ID NO: 5955; SEQ ID NO: 5956; SEQ ID NO: 5957; SEQ ID NO: 5958; SEQ ID NO: 5959; SEQ ID NO: 5960; SEQ ID NO: 5961; SEQ ID NO: 5962; SEQ ID NO: 5963; SEQ ID NO: 5964; SEQ ID NO: 5965; SEQ ID NO: 5966; SEQ ID NO: 5967; SEQ ID NO: 5968; SEQ ID NO: 5969; SEQ ID NO: 5970; SEQ ID NO: 5971; SEQ ID NO: 5972; SEQ ID NO: 5973; SEQ ID NO: 5974; SEQ ID NO: 5975; SEQ ID NO: 5976; SEQ ID NO: 5977; SEQ ID NO: 5978; SEQ ID NO: 5979; SEQ ID NO: 5980; SEQ ID NO: 5981; SEQ ID NO: 5982; SEQ ID NO: 5983; SEQ ID NO: 5984; SEQ ID NO: 5985; SEQ ID NO: 5986; SEQ ID NO: 5987; SEQ ID NO: 5988; SEQ ID NO: 5989; SEQ ID NO: 5990; SEQ ID NO: 5991; SEQ ID NO: 5992; SEQ ID NO: 5993; SEQ ID NO: 5994; SEQ ID NO: 5995; SEQ ID NO: 5996; SEQ ID NO: 5997; SEQ ID NO: 5998; SEQ ID NO: 5999; SEQ ID NO: 6000; SEQ ID NO: 6001; SEQ ID NO: 6002; SEQ ID NO: 6003; SEQ ID NO: 6004; SEQ ID NO: 6005; SEQ ID NO: 6006; SEQ ID NO: 6007; SEQ ID NO: 6008; SEQ ID NO: 6009; SEQ ID NO: 6010; SEQ ID NO: 6011; SEQ ID NO: 6012; SEQ ID NO: 6013; SEQ ID NO: 6014; SEQ ID NO: 6015; SEQ ID NO: 6016; SEQ ID NO: 6017; SEQ ID NO: 6018; SEQ ID NO: 6019; SEQ ID NO: 6020; SEQ ID NO: 6021; SEQ ID NO: 6022; SEQ ID NO: 6023; SEQ ID NO: 6024; SEQ ID NO: 6025; SEQ ID NO: 6026; SEQ ID NO: 6027; SEQ ID NO: 6028; SEQ ID NO: 6029; SEQ ID NO: 6030; SEQ ID NO: 6031; SEQ ID NO: 6032; SEQ ID NO: 6033; SEQ ID NO: 6034; SEQ ID NO: 6035; SEQ ID NO: 6036; SEQ ID NO: 6037; SEQ ID NO: 6038; SEQ ID NO: 6039; SEQ ID NO: 6040; SEQ ID NO: 6041; SEQ ID NO: 6042; SEQ ID NO: 6043; SEQ ID NO: 6044; SEQ ID NO: 6045; SEQ ID NO: 6046; SEQ ID NO: 6047; SEQ ID NO: 6048; SEQ ID NO: 6049; SEQ ID NO: 6050; SEQ ID NO: 6051; SEQ ID NO: 6052; SEQ ID NO: 6053; SEQ ID NO: 6054; SEQ ID NO: 6055; SEQ ID NO: 6056; SEQ ID NO: 6057; SEQ ID NO: 6058; SEQ ID NO: 6059; SEQ ID NO: 6060; SEQ ID NO: 6061; SEQ ID NO: 6062; SEQ ID NO: 6063; SEQ ID NO: 6064; SEQ ID NO: 6065; SEQ ID NO: 6066; SEQ ID NO: 6067; SEQ ID NO: 6068; SEQ ID NO: 6069; SEQ ID NO: 6070; SEQ ID NO: 6071; SEQ ID NO: 6072; SEQ ID NO: 6073; SEQ ID NO: 6074; SEQ ID NO: 6075; SEQ ID NO: 6076; SEQ ID NO: 6077; SEQ ID NO: 6078; SEQ ID NO: 6079; SEQ ID NO: 6080; SEQ ID NO: 6081; SEQ ID NO: 6082; SEQ ID NO: 6083; SEQ ID NO: 6084; SEQ ID NO: 6085; SEQ ID NO: 6086; SEQ ID NO: 6087; SEQ ID NO: 6088; SEQ ID NO: 6089; SEQ ID NO: 6090; SEQ ID NO: 6091; SEQ ID NO: 6092; SEQ ID NO: 6093; SEQ ID NO: 6094; SEQ ID NO: 6095; SEQ ID NO: 6096; SEQ ID NO: 6097; SEQ ID NO: 6098; SEQ ID NO: 6099; SEQ ID NO: 6100; SEQ ID NO: 6101; SEQ ID NO: 6102; SEQ ID NO: 6103; SEQ ID NO: 6104; SEQ ID NO: 6105; SEQ ID NO: 6106; SEQ ID NO: 6107; SEQ ID NO: 6108; SEQ ID NO: 6109; SEQ ID NO: 6110; SEQ ID NO: 6111; SEQ ID NO: 6112; SEQ ID NO: 6113; SEQ ID NO: 6114; SEQ ID NO: 6115; SEQ ID NO: 6116; SEQ ID NO: 6117; SEQ ID NO: 6118; SEQ ID NO: 6119; SEQ ID NO: 6120; SEQ ID NO: 6121; SEQ ID NO: 6122; SEQ ID NO: 6123; SEQ ID NO: 6124; SEQ ID NO: 6125; SEQ ID NO: 6126; SEQ ID NO: 6127; SEQ ID NO: 6128; SEQ ID NO: 6129; SEQ ID NO: 6130; SEQ ID NO: 6131; SEQ ID NO: 6132; SEQ ID NO: 6133; SEQ ID NO: 6134; SEQ ID NO: 6135; SEQ ID NO: 6136; SEQ ID NO: 6137; SEQ ID NO: 6138; SEQ ID NO: 6139; SEQ ID NO: 6140; SEQ ID NO: 6141; SEQ ID NO: 6142; SEQ ID NO: 6143; SEQ ID NO: 6144; SEQ ID NO: 6145; SEQ ID NO: 6146; SEQ ID NO: 6147; SEQ ID NO: 6148; SEQ ID NO: 6149; SEQ ID NO: 6150; SEQ ID NO: 6151; SEQ ID NO:6152; SEQ ID NO: 6153; SEQ ID NO: 6154; SEQ ID NO: 6155; SEQ ID NO: 6156; SEQ ID NO: 6157; SEQ ID NO: 6158; SEQ ID NO: 6159; SEQ ID NO: 6160; SEQ ID NO: 6161; SEQ ID NO: 6162; SEQ ID NO: 6163; SEQ ID NO: 6164; SEQ ID NO: 6165; SEQ ID NO: 6166; SEQ ID NO: 6167; SEQ ID NO: 6168; SEQ ID NO: 6169; SEQ ID NO: 6170; SEQ ID NO: 6171; SEQ ID NO: 6172; SEQ ID NO: 6173; SEQ ID NO: 6174; SEQ ID NO: 6175; SEQ ID NO: 6176; SEQ ID NO: 6177; SEQ ID NO: 6178; SEQ ID NO: 6179; SEQ ID NO: 6180; SEQ ID NO: 6181; SEQ ID NO: 6182; SEQ ID NO: 6183; SEQ ID NO: 6184; SEQ ID NO: 6185; SEQ ID NO: 6186; SEQ ID NO: 6187; SEQ ID NO: 6188; SEQ ID NO: 6189; SEQ ID NO: 6190; SEQ ID NO: 6191; SEQ ID NO: 6192; SEQ ID NO: 6193; SEQ ID NO: 6194; SEQ ID NO: 6195; SEQ ID NO: 6196; SEQ ID NO: 6197; SEQ ID NO: 6198; SEQ ID NO: 6199; SEQ ID NO: 6200; SEQ ID NO: 6201; SEQ ID NO: 6202; SEQ ID NO: 6203; SEQ ID NO: 6204; SEQ ID NO: 6205; SEQ ID NO: 6206; SEQ ID NO: 6207; SEQ ID NO: 6208; SEQ ID NO: 6209; SEQ ID NO: 6210; SEQ ID NO: 6211; SEQ ID NO: 6212; SEQ ID NO: 6213; SEQ ID NO: 6214; SEQ ID NO: 6215; SEQ ID NO: 6216; SEQ ID NO: 6217; SEQ ID NO: 6218; SEQ ID NO: 6219; SEQ ID NO: 6220; SEQ ID NO: 6221; SEQ ID NO: 6222; SEQ ID NO: 6223; SEQ ID NO: 6224; SEQ ID NO: 6225; SEQ ID NO: 6226; SEQ ID NO: 6227; SEQ ID NO: 6228; SEQ ID NO: 6229; SEQ ID NO: 6230; SEQ ID NO: 6231; SEQ ID NO: 6232; SEQ ID NO: 6233; SEQ ID NO: 6234; SEQ ID NO: 6235; SEQ ID NO: 6236; SEQ ID NO: 6237; SEQ ID NO: 6238; SEQ ID NO: 6239; SEQ ID NO: 6240; SEQ ID NO: 6241; SEQ ID NO: 6242; SEQ ID NO: 6243; SEQ ID NO: 6244; SEQ ID NO: 6245; SEQ ID NO: 6246; SEQ ID NO: 6247; SEQ ID NO: 6248; SEQ ID NO: 6249; SEQ ID NO: 6250; SEQ ID NO: 6251; SEQ ID NO: 6252; SEQ ID NO: 6253; SEQ ID NO: 6254; SEQ ID NO: 6255; SEQ ID NO: 6256; SEQ ID NO: 6257; SEQ ID NO: 6258; SEQ ID NO: 6259; SEQ ID NO: 6260; SEQ ID NO: 6261; SEQ ID NO: 6262; SEQ ID NO: 6263; SEQ ID NO: 6264; SEQ ID NO: 6265; SEQ ID NO: 6266; SEQ ID NO: 6267; SEQ ID NO: 6268; SEQ ID NO: 6269; SEQ ID NO: 6270; SEQ ID NO: 6271; SEQ ID NO: 6272; SEQ ID NO: 6273; SEQ ID NO: 6274; SEQ ID NO: 6275; SEQ ID NO: 6276; SEQ ID NO: 6277; SEQ ID NO: 6278; SEQ ID NO: 6279; SEQ ID NO: 6280; SEQ ID NO: 6281; SEQ ID NO: 6282; SEQ ID NO: 6283; SEQ ID NO: 6284; SEQ ID NO: 6285; SEQ ID NO: 6286; SEQ ID NO: 6287; SEQ ID NO: 6288; SEQ ID NO: 6289; SEQ ID NO: 6290; SEQ ID NO: 6291; SEQ ID NO: 6292; SEQ ID NO: 6293; SEQ ID NO: 6294; SEQ ID NO: 6295; SEQ ID NO: 6296; SEQ ID NO: 6297; SEQ ID NO: 6298; SEQ ID NO: 6299; SEQ ID NO: 6300; SEQ ID NO: 6301; SEQ ID NO: 6302; SEQ ID NO: 6303; SEQ ID NO: 6304; SEQ ID NO: 6305; SEQ ID NO: 6306; SEQ ID NO: 6307; SEQ ID NO: 6308; SEQ ID NO: 6309; SEQ ID NO: 6310; SEQ ID NO: 6311; SEQ ID NO: 6312; SEQ ID NO: 6313; SEQ ID NO: 6314; SEQ ID NO: 6315; SEQ ID NO: 6316; SEQ ID NO: 6317; SEQ ID NO: 6318; SEQ ID NO: 6319; SEQ ID NO: 6320; SEQ ID NO: 6321; SEQ ID NO: 6322; SEQ ID NO: 6323; SEQ ID NO: 6324; SEQ ID NO: 6325; SEQ ID NO: 6326; SEQ ID NO: 6327; SEQ ID NO: 6328; SEQ ID NO: 6329; SEQ ID NO: 6330; SEQ ID NO: 6331; SEQ ID NO: 6332; SEQ ID NO: 6333; SEQ ID NO: 6334; SEQ ID NO: 6335; SEQ ID NO: 6336; SEQ ID NO: 6337; SEQ ID NO: 9637; SEQ ID NO: 9638; SEQ ID NO: 9639; SEQ ID NO: 9640; SEQ ID NO: 9641; SEQ ID NO: 9642; SEQ ID NO: 9643; SEQ ID NO: 9644; SEQ ID NO: 9645; SEQ ID NO: 9646; SEQ ID NO: 9647; SEQ ID NO: 9648; SEQ ID NO: 9649; SEQ ID NO: 9650; SEQ ID NO: 9651; SEQ ID NO: 9652; SEQ ID NO: 9653; SEQ ID NO: 9654; SEQ ID NO: 9655; SEQ ID NO: 9656; SEQ ID NO: 9657; SEQ ID NO: 9658; SEQ ID NO: 9659; SEQ ID NO: 9660; SEQ ID NO: 9661; SEQ ID NO: 9662; SEQ ID NO: 9663; SEQ ID NO: 9664; SEQ ID NO: 9665; SEQ ID NO: 9666; SEQ ID NO: 9667; SEQ ID NO: 9668; SEQ ID NO: 9669; SEQ ID NO: 9670; SEQ ID NO: 9671; SEQ ID NO: 9672; SEQ ID NO: 9673; SEQ ID NO: 9674; SEQ ID NO: 9675; SEQ ID NO: 9676; SEQ ID NO: 9677; SEQ ID NO: 9678; SEQ ID NO: 9679; SEQ ID NO: 9680; SEQ ID NO: 9681; SEQ ID NO: 9682; SEQ ID NO: 9683; SEQ ID NO: 9684; SEQ ID NO: 9685; SEQ ID NO: 9686; SEQ ID NO: 9687; SEQ ID NO: 9688; SEQ ID NO: 9689; SEQ ID NO: 9690; SEQ ID NO: 9691; SEQ ID NO: 9692; SEQ ID NO: 9693; SEQ ID NO: 9694; SEQ ID NO: 9695; SEQ ID NO: 9696; SEQ ID NO: 9697; SEQ ID NO: 9698; SEQ ID NO: 9699; SEQ ID NO: 9700; SEQ ID NO: 9701; SEQ ID NO: 9702; SEQ ID NO: 9703 and SEQ ID NO: 9704.  
     [0098] In one embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  flagella-associated polypeptide or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 4763; SEQ ID NO: 4764; SEQ ID NO: 4765; SEQ ID NO: 4766; SEQ ID NO: 4767; SEQ ID NO: 4768; SEQ ID NO: 4769; SEQ ID NO: 4770; SEQ ID NO: 4771; SEQ ID NO: 4772; SEQ ID NO: 4773; SEQ ID NO: 4774; SEQ ID NO: 4775; SEQ ID NO: 4776; SEQ ID NO: 4777; SEQ ID NO: 4778; SEQ ID NO: 4779; SEQ ID NO: 4780; SEQ ID NO: 4781; SEQ ID NO: 4782; SEQ ID NO: 4783; SEQ ID NO: 4784; SEQ ID NO: 4785; SEQ ID NO: 4786; SEQ ID NO: 4787; SEQ ID NO: 4788; SEQ ID NO: 4789; SEQ ID NO: 4790; SEQ ID NO: 4791; SEQ ID NO: 4792; SEQ ID NO: 4793; SEQ ID NO: 4794; SEQ ID NO: 4795; SEQ ID NO: 4796; SEQ ID NO: 4797; SEQ ID NO: 4798; SEQ ID NO: 4799; SEQ ID NO: 4800; SEQ ID NO: 4801; SEQ ID NO: 4802; SEQ ID NO: 4803; SEQ ID NO: 4804; SEQ ID NO: 4805; SEQ ID NO: 4806; SEQ ID NO: 4807; SEQ ID NO: 4808; SEQ ID NO: 4809; SEQ ID NO: 4810; SEQ ID NO: 4811; SEQ ID NO: 4812; SEQ ID NO: 4813; SEQ ID NO: 4814; SEQ ID NO: 4815; SEQ ID NO: 4816; SEQ ID NO: 4817; SEQ ID NO: 4818; SEQ ID NO: 4819; SEQ ID NO: 4820; SEQ ID NO: 4821; SEQ ID NO: 4822; SEQ ID NO: 4823; SEQ ID NO: 4824; SEQ ID NO: 4825; SEQ ID NO: 4826; SEQ ID NO: 4827; SEQ ID NO: 4828; SEQ ID NO: 4829; SEQ ID NO: 4830; SEQ ID NO: 4831; SEQ ID NO: 4832; SEQ ID NO: 4833; SEQ ID NO: 4834; SEQ ID NO: 4835; SEQ ID NO: 4836; SEQ ID NO: 4837; SEQ ID NO: 4838; SEQ ID NO: 4839; SEQ ID NO: 4840; SEQ ID NO: 4841; SEQ ID NO: 4842; SEQ ID NO: 4843; SEQ ID NO: 4844; SEQ ID NO: 4845; SEQ ID NO: 4846; SEQ ID NO: 4847; SEQ ID NO: 4848; SEQ ID NO: 4849; SEQ ID NO: 4850; SEQ ID NO: 4851; SEQ ID NO: 4852; SEQ ID NO: 4853; SEQ ID NO: 4854; SEQ ID NO: 4855; SEQ ID NO: 4856; SEQ ID NO: 4857; SEQ ID NO: 4858; SEQ ID NO: 4859; SEQ ID NO: 4860; SEQ ID NO: 4861; SEQ ID NO: 4862; SEQ ID NO: 4863; SEQ ID NO: 4864; SEQ ID NO: 4865; SEQ ID NO: 9637; SEQ ID NO: 9638 and SEQ ID NO: 9639.  
     [0099] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  outer membrane polypeptide or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 4866; SEQ ID NO: 4867; SEQ ID NO: 4868; SEQ ID NO: 4869; SEQ ID NO: 4870; SEQ ID NO: 4871; SEQ ID NO: 4872; SEQ ID NO: 4873; SEQ ID NO: 4874; SEQ ID NO: 4875; SEQ ID NO: 4876; SEQ ID NO: 4877; SEQ ID NO: 4878; SEQ ID NO: 4879; SEQ ID NO: 4880; SEQ ID NO: 4881; SEQ ID NO: 4882; SEQ ID NO: 4883; SEQ ID NO: 4884; SEQ ID NO: 4885; SEQ ID NO: 4886; SEQ ID NO: 4887; SEQ ID NO: 4888; SEQ ID NO: 4889; SEQ ID NO: 4890; SEQ ID NO: 4891; SEQ ID NO: 4892; SEQ ID NO: 4893; SEQ ID NO: 4894; SEQ ID NO: 4895; SEQ ID NO: 4896; SEQ ID NO: 4897; SEQ ID NO: 4898; SEQ ID NO: 4899; SEQ ID NO: 4900; SEQ ID NO: 4901; SEQ ID NO: 4902; SEQ ID NO: 4903; SEQ ID NO: 4904; SEQ ID NO: 4905; SEQ ID NO: 4906; SEQ ID NO: 4907; SEQ ID NO: 4908; SEQ ID NO: 4909; SEQ ID NO: 4910; SEQ ID NO: 4911; SEQ ID NO: 4912; SEQ ID NO: 4913; SEQ ID NO: 4914; SEQ ID NO: 4915; SEQ ID NO: 4916; SEQ ID NO: 4917; SEQ ID NO: 4918; SEQ ID NO: 4919; SEQ ID NO: 4920; SEQ ID NO: 4921; SEQ ID NO: 4922; SEQ ID NO: 4923; SEQ ID NO: 4924; SEQ ID NO: 4925; SEQ ID NO: 4926; SEQ ID NO: 4927; SEQ ID NO: 4928; SEQ ID NO: 4929; SEQ ID NO: 4930; SEQ ID NO: 4931; SEQ ID NO: 4932; SEQ ID NO: 4933; SEQ ID NO: 4934; SEQ ID NO: 4935; SEQ ID NO: 4936; SEQ ID NO: 4937; SEQ ID NO: 4938; SEQ ID NO: 4939; SEQ ID NO: 4940; SEQ ID NO: 4941; SEQ ID NO: 4942; SEQ ID NO: 4943; SEQ ID NO: 4944; SEQ ID NO: 4945; SEQ ID NO: 4946; SEQ ID NO: 4947; SEQ ID NO: 4948; SEQ ID NO: 4949; SEQ ID NO: 4950; SEQ ID NO: 4951; SEQ ID NO: 4952; SEQ ID NO: 4953; SEQ ID NO: 4954; SEQ ID NO: 4955; SEQ ID NO: 4956; SEQ ID NO: 4957; SEQ ID NO: 4958; SEQ ID NO: 4959; SEQ ID NO: 4960; SEQ ID NO: 4961; SEQ ID NO: 4962; SEQ ID NO: 4963; SEQ ID NO: 4964; SEQ ID NO: 4965; SEQ ID NO: 4966; SEQ ID NO: 4967; SEQ ID NO: 4968; SEQ ID NO: 4969; SEQ ID NO: 4970; SEQ ID NO: 4971; SEQ ID NO: 4972; SEQ ID NO: 4973; SEQ ID NO: 4974; SEQ ID NO: 4975; SEQ ID NO: 4976; SEQ ID NO: 4977; SEQ ID NO: 4978; SEQ ID NO: 4979; SEQ ID NO: 4980; SEQ ID NO: 4981; SEQ ID NO: 4982; SEQ ID NO: 4983; SEQ ID NO: 4984; SEQ ID NO: 4985; SEQ ID NO: 4986; SEQ ID NO: 4987; SEQ ID NO: 4988; SEQ ID NO: 4989; SEQ ID NO: 4990; SEQ ID NO: 4991; SEQ ID NO: 4992; SEQ ID NO: 4993; SEQ ID NO: 4994; SEQ ID NO: 4995; SEQ ID NO: 4996; SEQ ID NO: 4997; SEQ ID NO: 4998; SEQ ID NO: 4999; SEQ ID NO: 5000; SEQ ID NO: 5001; SEQ ID NO: 5002; SEQ ID NO: 5003; SEQ ID NO: 5004; SEQ ID NO: 5005; SEQ ID NO: 5006; SEQ ID NO: 5007; SEQ ID NO: 5008; SEQ ID NO: 5009; SEQ ID NO: 5010; SEQ ID NO: 5011; SEQ ID NO: 5012; SEQ ID NO: 5013; SEQ ID NO: 5014; SEQ ID NO: 5015; SEQ ID NO: 5016; SEQ ID NO: 5017; SEQ ID NO: 5018; SEQ ID NO: 5019; SEQ ID NO: 5020; SEQ ID NO: 5021; SEQ ID NO: 5022; SEQ ID NO: 5023; SEQ ID NO: 5024; SEQ ID NO: 5025; SEQ ID NO: 5026; SEQ ID NO: 5027; SEQ ID NO: 5028; SEQ ID NO: 5029; SEQ ID NO: 5030; SEQ ID NO: 5031; SEQ ID NO: 5032; SEQ ID NO: 5033; SEQ ID NO: 5034; SEQ ID NO: 5035; SEQ ID NO: 5036; SEQ ID NO: 5037; SEQ ID NO: 5038; SEQ ID NO: 5039; SEQ ID NO: 5040; SEQ ID NO: 5041; SEQ ID NO: 5042; SEQ ID NO: 5043; SEQ ID NO: 5044; SEQ ID NO: 5045; SEQ ID NO: 5046; SEQ ID NO: 5047; SEQ ID NO: 5048; SEQ ID NO: 5049; SEQ ID NO: 5050; SEQ ID NO: 5051; SEQ ID NO: 5052; SEQ ID NO: 5053; SEQ ID NO: 5054; SEQ ID NO: 5055; SEQ ID NO: 5056; SEQ ID NO: 5057; SEQ ID NO: 5058; SEQ ID NO: 5059; SEQ ID NO: 5060; SEQ ID NO: 5061; SEQ ID NO: 5062; SEQ ID NO: 5063; SEQ ID NO: 5064; SEQ ID NO: 5065; SEQ ID NO: 5066; SEQ ID NO: 5067; SEQ ID NO: 5068; SEQ ID NO: 5069; SEQ ID NO: 5070; SEQ ID NO: 5071; SEQ ID NO: 5072; SEQ ID NO: 5073; SEQ ID NO: 5074; SEQ ID NO: 5075; SEQ ID NO: 5076; SEQ ID NO: 5077; SEQ ID NO: 5078; SEQ ID NO: 5079; SEQ ID NO: 5080; SEQ ID NO: 5081; SEQ ID NO: 5082; SEQ ID NO: 5083; SEQ ID NO: 5084; SEQ ID NO: 5085; SEQ ID NO: 5086; SEQ ID NO: 5087; SEQ ID NO: 5088; SEQ ID NO: 5089; SEQ ID NO: 5090; SEQ ID NO: 5091; SEQ ID NO: 5092; SEQ ID NO: 5093; SEQ ID NO: 5094; SEQ ID NO: 5095; SEQ ID NO: 5096; SEQ ID NO: 5097; SEQ ID NO: 5098; SEQ ID NO: 5099; SEQ ID NO: 5100; SEQ ID NO: 5101; SEQ ID NO: 5102; SEQ ID NO: 5103; SEQ ID NO: 5104; SEQ ID NO: 5105; SEQ ID NO: 5106; SEQ ID NO: 5107; SEQ ID NO: 5108; SEQ ID NO: 5109; SEQ ID NO: 5110; SEQ ID NO: 5111; SEQ ID NO: 5112; SEQ ID NO: 5113; SEQ ID NO: 5114; SEQ ID NO: 5115; SEQ ID NO: 5116; SEQ ID NO: 5117; SEQ ID NO: 5118; SEQ ID NO: 5119; SEQ ID NO: 5120; SEQ ID NO: 5121; SEQ ID NO: 5122; SEQ ID NO: 5123; SEQ ID NO: 5124; SEQ ID NO: 5125; SEQ ID NO: 5126; SEQ ID NO: 5127; SEQ ID NO: 5128; SEQ ID NO: 5129; SEQ ID NO: 5130; SEQ ID NO: 5131; SEQ ID NO: 5132; SEQ ID NO: 5133; SEQ ID NO: 5134; SEQ ID NO: 5135; SEQ ID NO: 5136; SEQ ID NO: 5137; SEQ ID NO: 5138; SEQ ID NO: 5139; SEQ ID NO: 5140; SEQ ID NO: 5141; SEQ ID NO: 5142; SEQ ID NO: 5143; SEQ ID NO: 5144; SEQ ID NO: 5145; SEQ ID NO: 5146; SEQ ID NO: 5147; SEQ ID NO: 5148; SEQ ID NO: 5149; SEQ ID NO: 5150; SEQ ID NO: 5151; SEQ ID NO: 5152; SEQ ID NO: 5153; SEQ ID NO: 5154; SEQ ID NO: 5155; SEQ ID NO: 5156; SEQ ID NO: 5157; SEQ ID NO: 5158; SEQ ID NO: 5159; SEQ ID NO: 5160; SEQ ID NO: 5161; SEQ ID NO: 5162; SEQ ID NO: 5163; SEQ ID NO: 5164; SEQ ID NO: 5165; SEQ ID NO: 5166; SEQ ID NO: 5167; SEQ ID NO: 5168; SEQ ID NO: 5169; SEQ ID NO: 5170; SEQ ID NO: 5171; SEQ ID NO: 5172; SEQ ID NO: 5173; SEQ ID NO: 5174; SEQ ID NO: 5175; SEQ ID NO: 5176; SEQ ID NO: 5177; SEQ ID NO: 5178; SEQ ID NO: 5179; SEQ ID NO: 5180; SEQ ID NO: 5181; SEQ ID NO: 5182; SEQ ID NO: 5183; SEQ ID NO: 5184; SEQ ID NO: 5185; SEQ ID NO: 5186; SEQ ID NO: 5187; SEQ ID NO: 5188; SEQ ID NO: 5189; SEQ ID NO: 5190; SEQ ID NO: 5191; SEQ ID NO: 5192; SEQ ID NO: 5193; SEQ ID NO: 5194; SEQ ID NO: 5195; SEQ ID NO: 5196; SEQ ID NO: 5197; SEQ ID NO: 5198; SEQ ID NO: 5199; SEQ ID NO: 5200; SEQ ID NO: 5201; SEQ ID NO: 5202; SEQ ID NO: 5203; SEQ ID NO: 5204; SEQ ID NO: 5205; SEQ ID NO: 5206; SEQ ID NO: 5207; SEQ ID NO: 5208; SEQ ID NO: 5209; SEQ ID NO: 5210; SEQ ID NO: 5211; SEQ ID NO: 5212; SEQ ID NO: 5213; SEQ ID NO: 5214; SEQ ID NO: 5215; SEQ ID NO: 5216; SEQ ID NO: 5217; SEQ ID NO: 5218; SEQ ID NO: 5219; SEQ ID NO: 5220; SEQ ID NO: 5221; SEQ ID NO: 5222; SEQ ID NO: 5223; SEQ ID NO: 5224; SEQ ID NO: 5225; SEQ ID NO: 5226; SEQ ID NO: 5227; SEQ ID NO: 5228; SEQ ID NO: 5229; SEQ ID NO: 5230; SEQ ID NO: 5231; SEQ ID NO: 5232; SEQ ID NO: 5233; SEQ ID NO: 5234; SEQ ID NO: 5235; SEQ ID NO: 5236; SEQ ID NO: 5237; SEQ ID NO: 5238; SEQ ID NO: 5239; SEQ ID NO: 5240; SEQ ID NO: 5241; SEQ ID NO: 5242; SEQ ID NO: 5243; SEQ ID NO: 5244; SEQ ID NO: 5245; SEQ ID NO: 5246; SEQ ID NO: 5247; SEQ ID NO: 5248; SEQ ID NO: 5249; SEQ ID NO: 5250; SEQ ID NO: 5251; SEQ ID NO: 5252; SEQ ID NO: 5253; SEQ ID NO: 5254; SEQ ID NO: 5255; SEQ ID NO: 5256; SEQ ID NO: 5257; SEQ ID NO: 5258; SEQ ID NO: 5259; SEQ ID NO: 5260; SEQ ID NO: 5261; SEQ ID NO: 5262; SEQ ID NO: 5263; SEQ ID NO: 5264; SEQ ID NO: 5265; SEQ ID NO: 5266; SEQ ID NO: 5267; SEQ ID NO: 5268; SEQ ID NO: 5269; SEQ ID NO: 5270; SEQ ID NO: 5271 and SEQ ID NO: 5272.  
     [0100] In another embodiment, the  H. pylori  outer membrane polypeptide or a fragment thereof is an  H. pylori  polypeptide having a terminal phenylalanine residue or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 4866; SEQ ID NO: 4867; SEQ ID NO: 4868; SEQ ID NO: 4869; SEQ ID NO: 4870; SEQ ID NO: 4871; SEQ ID NO: 4872; SEQ ID NO: 4873; SEQ ID NO: 4874; SEQ ID NO: 4875; SEQ ID NO: 4876; SEQ ID NO: 4877; SEQ ID NO: 4878; SEQ ID NO: 4879; SEQ ID NO: 4880; SEQ ID NO: 4881; SEQ ID NO: 4882; SEQ ID NO: 4883; SEQ ID NO: 4884; SEQ ID NO: 4885; SEQ ID NO: 4886; SEQ ID NO: 4887; SEQ ID NO: 4888; SEQ ID NO: 4889; SEQ ID NO: 4890; SEQ ID NO: 4891; SEQ ID NO: 4892; SEQ ID NO: 4893; SEQ ID NO: 4894; SEQ ID NO: 4895; SEQ ID NO: 4896; SEQ ID NO: 4897; SEQ ID NO: 4898; SEQ ID NO: 4899; SEQ ID NO: 4900; SEQ ID NO: 4901; SEQ ID NO: 4902; SEQ ID NO: 4903; SEQ ID NO: 4904; SEQ ID NO: 4905; SEQ ID NO: 4906; SEQ ID NO: 4907; SEQ ID NO: 4908; SEQ ID NO: 4909; SEQ ID NO: 4910; SEQ ID NO: 4911; SEQ ID NO: 4912; SEQ ID NO: 4913; SEQ ID NO: 4914; SEQ ID NO: 4915; SEQ ID NO: 4916; SEQ ID NO: 4917; SEQ ID NO: 4918; SEQ ID NO: 4919; SEQ ID NO: 4920; SEQ ID NO: 4921; SEQ ID NO: 4922; SEQ ID NO: 4923; SEQ ID NO: 4924; SEQ ID NO: 4925; SEQ ID NO: 4926; SEQ ID NO: 4927; SEQ ID NO: 4928; SEQ ID NO: 4929; SEQ ID NO: 4930; SEQ ID NO: 4931; SEQ ID NO: 4932; SEQ ID NO: 4933; SEQ ID NO: 4934; SEQ ID NO: 4935; SEQ ID NO: 4936; SEQ ID NO: 4937; SEQ ID NO: 4938; SEQ ID NO: 4939; SEQ ID NO: 4940; SEQ ID NO: 4941; SEQ ID NO: 4942; SEQ ID NO: 4943; SEQ ID NO: 4944; SEQ ID NO: 4945; SEQ ID NO: 4946; SEQ ID NO: 4947; SEQ ID NO: 4948; SEQ ID NO: 4949; SEQ ID NO: 4950; SEQ ID NO: 4951; SEQ ID NO: 4952; SEQ ID NO: 4953; SEQ ID NO: 4954; SEQ ID NO: 4955; SEQ ID NO: 4956; SEQ ID NO: 4957; SEQ ID NO: 4958; SEQ ID NO: 4959; SEQ ID NO: 4960; SEQ ID NO: 4961; SEQ ID NO: 4962; SEQ ID NO: 4963; SEQ ID NO: 4964; SEQ ID NO: 4965; SEQ ID NO: 4966; SEQ ID NO: 4967; SEQ ID NO: 4968; SEQ ID NO: 4969; SEQ ID NO: 4970; SEQ ID NO: 4971; SEQ ID NO: 4972; SEQ ID NO: 4973; SEQ ID NO: 4974; SEQ ID NO: 4975; SEQ ID NO: 4976; SEQ ID NO: 4977; SEQ ID NO: 4978; SEQ ID NO: 4979; SEQ ID NO: 4980; SEQ ID NO: 4981; SEQ ID NO: 4982; SEQ ID NO: 4983; SEQ ID NO: 4984; SEQ ID NO: 4985; SEQ ID NO: 4986; SEQ ID NO: 4987; SEQ ID NO: 4988; SEQ ID NO: 4989; SEQ ID NO: 4990; SEQ ID NO: 4991; SEQ ID NO: 4992; SEQ ID NO: 4993; SEQ ID NO: 4994; SEQ ID NO: 4995; SEQ ID NO: 4996; SEQ ID NO: 4997; SEQ ID NO: 4998; SEQ ID NO: 4999; SEQ ID NO: 5000; SEQ ID NO: 5001; SEQ ID NO: 5002; SEQ ID NO: 5003; SEQ ID NO: 5004; SEQ ID NO: 5005; SEQ ID NO: 5006; SEQ ID NO: 5007; SEQ ID NO: 5008; SEQ ID NO: 5009; SEQ ID NO: 5010; SEQ ID NO: 5011; SEQ ID NO: 5012; SEQ ID NO: 5013; SEQ ID NO: 5014; SEQ ID NO: 5015; SEQ ID NO: 5016; SEQ ID NO: 5017; SEQ ID NO: 5018; SEQ ID NO: 5019; SEQ ID NO: 5020; SEQ ID NO: 5021; SEQ ID NO: 5022; SEQ ID NO: 5023; SEQ ID NO: 5024; SEQ ID NO: 5025; SEQ ID NO: 5026; SEQ ID NO: 5027; SEQ ID NO: 5028; SEQ ID NO: 5029; SEQ ID NO: 5030; SEQ ID NO: 5031; SEQ ID NO: 5032; SEQ ID NO: 5033; SEQ ID NO: 5034; SEQ ID NO: 5035; SEQ ID NO: 5036; SEQ ID NO: 5037; SEQ ID NO: 5038; SEQ ID NO: 5039; SEQ ID NO: 5040; SEQ ID NO: 5041; SEQ ID NO: 5042; SEQ ID NO: 5043; SEQ ID NO: 5044; SEQ ID NO: 5045; SEQ ID NO: 5046; SEQ ID NO: 5047; SEQ ID NO: 5048; SEQ ID NO: 5049; SEQ ID NO: 5050; SEQ ID NO: 5051; SEQ ID NO: 5052; SEQ ID NO: 5053; SEQ ID NO: 5054; SEQ ID NO: 5055; SEQ ID NO: 5056; SEQ ID NO: 5057; SEQ ID NO: 5058; SEQ ID NO: 5059; SEQ ID NO: 5060; SEQ ID NO: 5061; SEQ ID NO: 5062; SEQ ID NO: 5063; SEQ ID NO: 5064; SEQ ID NO: 5065; SEQ ID NO: 5066; SEQ ID NO: 5067; SEQ ID NO: 5068; SEQ ID NO: 5069; SEQ ID NO: 5070; SEQ ID NO: 5071; SEQ ID NO: 5072; SEQ ID NO: 5073; SEQ ID NO: 5074; SEQ ID NO: 5075; SEQ ID NO: 5076; SEQ ID NO: 5077; SEQ ID NO: 5078; SEQ ID NO: 5079; SEQ ID NO: 5080; SEQ ID NO: 5081; SEQ ID NO: 5082; SEQ ID NO: 5083; SEQ ID NO: 5084; SEQ ID NO: 5085; SEQ ID NO: 5086; SEQ ID NO: 5087; SEQ ID NO: 5088; SEQ ID NO: 5089; SEQ ID NO: 5090; SEQ ID NO: 5091; SEQ ID NO: 5092; SEQ ID NO: 5093; SEQ ID NO: 5094; SEQ ID NO: 5095; SEQ ID NO: 5104; SEQ ID NO: 5105; SEQ ID NO: 5106; SEQ ID NO: 5107; SEQ ID NO: 5108; SEQ ID NO: 5109; SEQ ID NO: 5110; SEQ ID NO: 5111; SEQ ID NO: 5112; SEQ ID NO: 5113; SEQ ID NO: 5114; SEQ ID NO: 5115; SEQ ID NO: 5116; SEQ ID NO: 5117; SEQ ID NO: 5118; SEQ ID NO: 5119; SEQ ID NO: 5120; SEQ ID NO: 5121; SEQ ID NO: 5122; SEQ ID NO: 5123; SEQ ID NO: 5124; SEQ ID NO: 5131; SEQ ID NO: 5132; SEQ ID NO: 5133; SEQ ID NO: 5134; SEQ ID NO: 5135; SEQ ID NO: 5136; SEQ ID NO: 5137; SEQ ID NO: 5138; SEQ ID NO: 5139 SEQ ID NO: 5140; SEQ ID NO: 5141; SEQ ID NO: 5142; SEQ ID NO: 5143; SEQ ID NO: 5144; SEQ ID NO: 5145; SEQ ID NO: 5146; SEQ ID NO: 5147; SEQ ID NO: 5148; SEQ ID NO: 5149; SEQ ID NO: 5150; SEQ ID NO: 5151; SEQ ID NO: 5152; SEQ ID NO: 5153; SEQ ID NO: 5154; SEQ ID NO: 5155; SEQ ID NO: 5156; SEQ ID NO: 5157; SEQ ID NO: 5158; SEQ ID NO: 5159; SEQ ID NO: 9640; SEQ ID NO: 9641; SEQ ID NO: 9642; SEQ ID NO: 9643; SEQ ID NO: 9644; SEQ ID NO: 9645; SEQ ID NO: 9646; SEQ ID NO: 9647 and SEQ ID NO: 9648.  
     [0101] In another embodiment, the  H. pylori  outer membrane polypeptide or a fragment thereof is an  H. pylori  polypeptide having a terminal phenylalanine residue and a C-terminal tyrosine cluster or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 5088; SEQ ID NO: 5089; SEQ ID NO: 5090; SEQ ID NO: 5091; SEQ ID NO: 5092; SEQ ID NO: 5093; SEQ ID NO: 5094; SEQ ID NO: 5095; SEQ ID NO: 5104; SEQ ID NO: 5105; SEQ ID NO: 5106; SEQ ID NO: 5107; SEQ ID NO: 5108; SEQ ID NO: 5109; SEQ ID NO: 5110; SEQ ID NO: 5111; SEQ ID NO: 5112; SEQ ID NO: 5113; SEQ ID NO: 5114; SEQ ID NO: 5115; SEQ ID NO: 5116; SEQ ID NO: 5117; SEQ ID NO: 5118; SEQ ID NO: 5119; SEQ ID NO: 5120; SEQ ID NO: 5121; SEQ ID NO: 5122; SEQ ID NO: 5123; SEQ ID NO: 5124; SEQ ID NO: 5131; SEQ ID NO: 5132; SEQ ID NO: 5133; SEQ ID NO: 5134; SEQ ID NO: 5135; SEQ ID NO: 5136; SEQ ID NO: 5137; SEQ ID NO: 5138; SEQ ID NO: 5139; SEQ ID NO: 5140; SEQ ID NO: 5141; SEQ ID NO: 5142; SEQ ID NO: 5143; SEQ ID NO: 5144; SEQ ID NO: 5145; SEQ ID NO: 5146; SEQ ID NO: 5147; SEQ ID NO: 5148; SEQ ID NO: 5149; SEQ ID NO: 5150; SEQ ID NO: 5151; SEQ ID NO: 5152; SEQ ID NO: 5153; SEQ ID NO: 5154; SEQ ID NO: 5155; SEQ ID NO: 5156; SEQ ID NO: 5157; SEQ ID NO: 5158; SEQ ID NO: 5159 and SEQ ID NO: 9648.  
     [0102] In another embodiment, the  H. pylori  outer membrane polypeptide or a fragment thereof is an  H. pylori  polypeptide having a terminal tyrosine cluster motif or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 5088; SEQ ID NO: 5089; SEQ ID NO: 5090; SEQ ID NO: 5091; SEQ ID NO: 5092; SEQ ID NO: 5093; SEQ ID NO: 5094; SEQ ID NO: 5095; SEQ ID NO: 5096; SEQ ID NO: 5097; SEQ ID NO: 5098; SEQ ID NO: 5099; SEQ ID NO: 5100; SEQ ID NO: 5101; SEQ ID NO: 5102; SEQ ID NO: 5103; SEQ ID NO: 5104; SEQ ID NO: 5105; SEQ ID NO: 5106; SEQ ID NO: 5107; SEQ ID NO: 5108; SEQ ID NO: 5109; SEQ ID NO: 5110; SEQ ID NO: 5111; SEQ ID NO: 5112; SEQ ID NO: 5113; SEQ ID NO: 5114; SEQ ID NO: 5115; SEQ ID NO: 5116; SEQ ID NO: 5117; SEQ ID NO: 5118; SEQ ID NO: 5119; SEQ ID NO: 5120; SEQ ID NO: 5121; SEQ ID NO: 5122; SEQ ID NO: 5123; SEQ ID NO: 5124; SEQ ID NO: 5125; SEQ ID NO: 5126; SEQ ID NO: 5127; SEQ ID NO: 5128; SEQ ID NO: 5129; SEQ ID NO: 5130; SEQ ID NO: 5131; SEQ ID NO: 5132; SEQ ID NO: 5133; SEQ ID NO: 5134; SEQ ID NO: 5135; SEQ ID NO: 5136; SEQ ID NO: 5137; SEQ ID NO: 5138; SEQ ID NO: 5139; SEQ ID NO: 5140; SEQ ID NO: 5141; SEQ ID NO: 5142; SEQ ID NO: 5143; SEQ ID NO: 5144; SEQ ID NO: 5145; SEQ ID NO: 5146; SEQ ID NO: 5147; SEQ ID NO: 5148; SEQ ID NO: 5149; SEQ ID NO: 5150; SEQ ID NO: 5151; SEQ ID NO: 5152; SEQ ID NO: 5153; SEQ ID NO: 5154; SEQ ID NO: 5155; SEQ ID NO: 5156; SEQ ID NO: 5157; SEQ ID NO: 5158; SEQ ID NO: 5159; SEQ ID NO: 5160; SEQ ID NO: 5161; SEQ ID NO: 5162; SEQ ID NO: 5163; SEQ ID NO: 5164; SEQ ID NO: 5165; SEQ ID NO: 5166; SEQ ID NO: 5167; SEQ ID NO: 5168; SEQ ID NO: 5169; SEQ ID NO: 5170; SEQ ID NO: 5171; SEQ ID NO: 5172; SEQ ID NO: 5173; SEQ ID NO: 5174; SEQ ID NO: 5175; SEQ ID NO: 5176; SEQ ID NO: 5177; SEQ ID NO: 5178; SEQ ID NO: 5186; SEQ ID NO: 5187; and SEQ ID NO: 9648.  
     [0103] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 5273; SEQ ID NO: 5274; SEQ ID NO: 5275; SEQ ID NO: 5276; SEQ ID NO: 5277; SEQ ID NO: 5278; SEQ ID NO: 5279; SEQ ID NO: 5280; SEQ ID NO: 5281; SEQ ID NO: 5282; SEQ ID NO: 5283; SEQ ID NO: 5284; SEQ ID NO: 5285; SEQ ID NO: 5286; SEQ ID NO: 5287; SEQ ID NO: 5288; SEQ ID NO: 5289; SEQ ID NO: 5290; SEQ ID NO: 5291; SEQ ID NO: 5292; SEQ ID NO: 5293; SEQ ID NO: 5294; SEQ ID NO: 5295; SEQ ID NO: 5296; SEQ ID NO: 5297; SEQ ID NO: 5298; SEQ ID NO: 5299; SEQ ID NO: 5300; SEQ ID NO: 5301; SEQ ID NO: 5302; SEQ ID NO: 5303; SEQ ID NO: 5304; SEQ ID NO: 5305; SEQ ID NO: 5306; SEQ ID NO: 5307; SEQ ID NO: 5308; SEQ ID NO: 5309; SEQ ID NO: 5310; SEQ ID NO: 5311; SEQ ID NO: 5312; SEQ ID NO: 5313; SEQ ID NO: 5314; SEQ ID NO: 5315; SEQ ID NO: 5316; SEQ ID NO: 5317; SEQ ID NO: 5318; SEQ ID NO: 5319; SEQ ID NO: 5320; SEQ ID NO: 5321; SEQ ID NO: 5322; SEQ ID NO: 5323; SEQ ID NO: 5324; SEQ ID NO: 5325; SEQ ID NO: 5326; SEQ ID NO: 5327; SEQ ID NO: 5328; SEQ ID NO: 5329; SEQ ID NO: 5330; SEQ ID NO: 5331; SEQ ID NO: 5332; SEQ ID NO: 5333; SEQ ID NO: 5334; SEQ ID NO: 5335; SEQ ID NO: 5336; SEQ ID NO: 5337; SEQ ID NO: 5338; SEQ ID NO: 5339; SEQ ID NO: 5340; SEQ ID NO: 5341; SEQ ID NO: 5342; SEQ ID NO: 5343; SEQ ID NO: 5344; SEQ ID NO: 5345; SEQ ID NO: 5346; SEQ ID NO: 5347; SEQ ID NO: 5348; SEQ ID NO: 5349; SEQ ID NO: 5350; SEQ ID NO: 5351; SEQ ID NO: 5352; SEQ ID NO: 5353; SEQ ID NO: 5354; SEQ ID NO: 5355; SEQ ID NO: 5356; SEQ ID NO: 5357; SEQ ID NO: 5358; SEQ ID NO: 5359; SEQ ID NO: 5360; SEQ ID NO: 5361; SEQ ID NO: 5362; SEQ ID NO: 5363; SEQ ID NO: 5364; SEQ ID NO: 5365; SEQ ID NO: 5366; SEQ ID NO: 5367; SEQ ID NO: 5368; SEQ ID NO: 5369; SEQ ID NO: 5370; SEQ ID NO: 5371; SEQ ID NO: 5372; SEQ ID NO: 5373; SEQ ID NO: 5374; SEQ ID NO: 5375; SEQ ID NO: 5376; SEQ ID NO: 5377; SEQ ID NO: 5378; SEQ ID NO: 5379; SEQ ID NO: 5380; SEQ ID NO: 5381; SEQ ID NO: 5382; SEQ ID NO: 5383; SEQ ID NO: 5384; SEQ ID NO: 5385; SEQ ID NO: 5386; SEQ ID NO: 5387; SEQ ID NO: 5388; SEQ ID NO: 5389; SEQ ID NO: 5390; SEQ ID NO: 5391; SEQ ID NO: 5392; SEQ ID NO: 5393; SEQ ID NO: 5394; SEQ ID NO: 5395; SEQ ID NO: 5396; SEQ ID NO: 5397; SEQ ID NO: 5398; SEQ ID NO: 5399; SEQ ID NO: 5400; SEQ ID NO: 5401; SEQ ID NO: 5402; SEQ ID NO: 5403; SEQ ID NO: 5404; SEQ ID NO: 5405; SEQ ID NO: 5406; SEQ ID NO: 5407; SEQ ID NO: 5408; SEQ ID NO: 5409; SEQ ID NO: 5410; SEQ ID NO: 5411; SEQ ID NO: 5412; SEQ ID NO: 5413; SEQ ID NO: 5414; SEQ ID NO: 5415; SEQ ID NO: 5416; SEQ ID NO: 5417; SEQ ID NO: 5418; SEQ ID NO: 5419; SEQ ID NO: 5420; SEQ ID NO: 5421; SEQ ID NO: 5422; SEQ ID NO: 5423; SEQ ID NO: 5424; SEQ ID NO: 5425; SEQ ID NO: 5426; SEQ ID NO: 5427; SEQ ID NO: 5428; SEQ ID NO: 5429; SEQ ID NO: 5430; SEQ ID NO: 5431; SEQ ID NO: 5432; SEQ ID NO: 5433; SEQ ID NO: 5434; SEQ ID NO: 5435; SEQ ID NO: 5436; SEQ ID NO: 5437; SEQ ID NO: 5438; SEQ ID NO: 5439; SEQ ID NO: 5440; SEQ ID NO: 5441; SEQ ID NO: 5442; SEQ ID NO: 5443; SEQ ID NO: 5444; SEQ ID NO: 5445; SEQ ID NO: 5446; SEQ ID NO: 5447; SEQ ID NO: 5448; SEQ ID NO: 5449; SEQ ID NO: 5450; SEQ ID NO: 5451; SEQ ID NO: 5452; SEQ ID NO: 5453; SEQ ID NO: 5454; SEQ ID NO: 5455; SEQ ID NO: 5456; SEQ ID NO: 5457; SEQ ID NO: 5458; SEQ ID NO: 5459; SEQ ID NO: 5460; SEQ ID NO: 5461; SEQ ID NO: 5462; SEQ ID NO: 5463; SEQ ID NO: 5464; SEQ ID NO: 5465; SEQ ID NO: 5466; SEQ ID NO:5467; SEQ ID NO: 5468; SEQ ID NO: 5469; SEQ ID NO: 5470; SEQ ID NO: 5471; SEQ ID NO: 5472; SEQ ID NO: 5473; SEQ ID NO: 5474; SEQ ID NO: 5475; SEQ ID NO: 5476; SEQ ID NO: 5477; SEQ ID NO: 5478; SEQ ID NO: 5479; SEQ ID NO: 5480; SEQ ID NO: 5481; SEQ ID NO: 5482; SEQ ID NO: 5483; SEQ ID NO: 5484; SEQ ID NO: 5485; SEQ ID NO: 5486; SEQ ID NO: 5487; SEQ ID NO: 5488; SEQ ID NO: 5489; SEQ ID NO: 5490; SEQ ID NO: 5491; SEQ ID NO: 5492; SEQ ID NO: 5493; SEQ ID NO: 5494; SEQ ID NO: 5495; SEQ ID NO: 5496; SEQ ID NO: 5497; SEQ ID NO: 5498; SEQ ID NO: 5499; SEQ ID NO: 5500; SEQ ID NO: 5501; SEQ ID NO: 5502; SEQ ID NO: 5503; SEQ ID NO: 5504; SEQ ID NO: 5505; SEQ ID NO: 5506; SEQ ID NO: 5507; SEQ ID NO: 5508; SEQ ID NO: 5509; SEQ ID NO: 5510; SEQ ID NO: 5511; SEQ ID NO: 5512; SEQ ID NO: 5513; SEQ ID NO: 5514; SEQ ID NO: 5515; SEQ ID NO: 5516; SEQ ID NO: 5517; SEQ ID NO: 5518; SEQ ID NO: 5519; SEQ ID NO: 5520; SEQ ID NO: 5521; SEQ ID NO: 5522; SEQ ID NO: 5523; SEQ ID NO: 5524; SEQ ID NO: 5525; SEQ ID NO: 5526; SEQ ID NO: 5527; SEQ ID NO: 5528; SEQ ID NO: 5529; SEQ ID NO: 5530; SEQ ID NO: 5531; SEQ ID NO: 5532; SEQ ID NO: 5533; SEQ ID NO: 5534; SEQ ID NO: 5535; SEQ ID NO: 5536; SEQ ID NO: 5537; SEQ ID NO: 5538; SEQ ID NO: 5539; SEQ ID NO: 5540; SEQ ID NO: 5541; SEQ ID NO: 5542; SEQ ID NO: 5543; SEQ ID NO: 5544; SEQ ID NO: 5545; SEQ ID NO: 5546; SEQ ID NO: 5547; SEQ ID NO: 5548; SEQ ID NO: 5549; SEQ ID NO: 5550; SEQ ID NO: 5551; SEQ ID NO: 5552; SEQ ID NO: 5553; SEQ ID NO: 5554; SEQ ID NO: 5555; SEQ ID NO: 5556; SEQ ID NO: 5557; SEQ ID NO: 5558; SEQ ID NO: 5559; SEQ ID NO: 5560; SEQ ID NO: 5561; SEQ ID NO: 5562; SEQ ID NO: 5563; SEQ ID NO: 5564; SEQ ID NO: 5565; SEQ ID NO: 5566; SEQ ID NO: 5567; SEQ ID NO: 5568; SEQ ID NO: 5569; SEQ ID NO: 5570; SEQ ID NO: 5571; SEQ ID NO: 5572; SEQ ID NO: 5573; SEQ ID NO: 5574; SEQ ID NO: 5575; SEQ ID NO: 5576; SEQ ID NO: 5577; SEQ ID NO: 5578; SEQ ID NO: 5579; SEQ ID NO: 5580; SEQ ID NO: 5581; SEQ ID NO: 5582; SEQ ID NO: 5583; SEQ ID NO: 5584; SEQ ID NO: 5585; SEQ ID NO: 5586; SEQ ID NO: 5587; SEQ ID NO: 5588; SEQ ID NO: 5589; SEQ ID NO: 5590; SEQ ID NO: 5591; SEQ ID NO: 5592; SEQ ID NO: 5593; SEQ ID NO: 5594; SEQ ID NO: 5595; SEQ ID NO: 5596; SEQ ID NO: 5597; SEQ ID NO: 5598; SEQ ID NO: 5599; SEQ ID NO: 5600; SEQ ID NO: 5601; SEQ ID NO: 5602; SEQ ID NO: 5603; SEQ ID NO: 5604; SEQ ID NO: 5605; SEQ ID NO: 5606; SEQ ID NO: 5607; SEQ ID NO: 5608; SEQ ID NO: 5609; SEQ ID NO: 5610; SEQ ID NO: 5611; SEQ ID NO: 5612; SEQ ID NO: 5613; SEQ ID NO: 5614; SEQ ID NO: 5615; SEQ ID NO: 5616; SEQ ID NO: 5617; SEQ ID NO: 5618; SEQ ID NO: 5619; SEQ ID NO: 5620; SEQ ID NO: 5621; SEQ ID NO: 5622; SEQ ID NO: 5623; SEQ ID NO: 5624; SEQ ID NO: 5625; SEQ ID NO: 5626; SEQ ID NO: 5627; SEQ ID NO: 5628; SEQ ID NO: 5629; SEQ ID NO: 5630; SEQ ID NO: 5631; SEQ ID NO: 5632; SEQ ID NO: 5633; SEQ ID NO: 5634; SEQ ID NO: 5635; SEQ ID NO: 5636; SEQ ID NO: 5637; SEQ ID NO: 5638; SEQ ID NO: 5639; SEQ ID NO: 5640; SEQ ID NO: 5641; SEQ ID NO: 5642; SEQ ID NO: 5643; SEQ ID NO: 5644; SEQ ID NO: 5645; SEQ ID NO: 5646; SEQ ID NO: 5647; SEQ ID NO: 5648; SEQ ID NO: 5649; SEQ ID NO: 5650; SEQ ID NO: 5651; SEQ ID NO: 5652; SEQ ID NO: 5653; SEQ ID NO: 5654; SEQ ID NO: 5655; SEQ ID NO: 5656; SEQ ID NO: 5657; SEQ ID NO: 5658; SEQ ID NO: 5659; SEQ ID NO: 5660; SEQ ID NO: 5661; SEQ ID NO: 5662; SEQ ID NO: 5663; SEQ ID NO: 5664; SEQ ID NO: 5665; SEQ ID NO: 5666; SEQ ID NO: 5667; SEQ ID NO: 5668; SEQ ID NO: 5669; SEQ ID NO: 5670; SEQ ID NO: 5671; SEQ ID NO: 5672; SEQ ID NO: 5673; SEQ ID NO: 5674; SEQ ID NO: 5675; SEQ ID NO: 5676; SEQ ID NO: 5677; SEQ ID NO: 5678; SEQ ID NO: 5679; SEQ ID NO: 5680; SEQ ID NO: 5681; SEQ ID NO: 5682; SEQ ID NO: 5683; SEQ ID NO: 5684; SEQ ID NO: 5685; SEQ ID NO: 5686; SEQ ID NO: 5687; SEQ ID NO: 5688; SEQ ID NO: 5689; SEQ ID NO: 5690; SEQ ID NO: 5691; SEQ ID NO: 5692; SEQ ID NO: 5693; SEQ ID NO: 5694; SEQ ID NO: 5695; SEQ ID NO: 5.696; SEQ ID NO: 5697; SEQ ID NO: 5698; SEQ ID NO: 5699; SEQ ID NO: 5700; SEQ ID NO: 5701; SEQ ID NO: 5702; SEQ ID NO: 5703; SEQ ID NO: 5704; SEQ ID NO: 5705; SEQ ID NO: 5706; SEQ ID NO: 5707; SEQ ID NO: 5708; SEQ ID NO: 5709; SEQ ID NO: 5710; SEQ ID NO: 5711; SEQ ID NO: 5712; SEQ ID NO: 5713; SEQ ID NO: 5714; SEQ ID NO: 5715; SEQ ID NO: 5716; SEQ ID NO: 5717; SEQ ID NO: 5718; SEQ ID NO: 5719; SEQ ID NO: 5720; SEQ ID NO: 57-21; SEQ ID NO: 5722; SEQ ID NO: 5723; SEQ ID NO: 5724; SEQ ID NO: 5725; SEQ ID NO: 5726; SEQ ID NO: 5727; SEQ ID NO: 5728; SEQ ID NO: 5729; SEQ ID NO: 5730; SEQ ID NO: 5731; SEQ ID NO: 5732; SEQ ID NO: 5733; SEQ ID NO: 5734; SEQ ID NO: 5735; SEQ ID NO: 5736; SEQ ID NO: 5737; SEQ ID NO: 5738; SEQ ID NO: 5739; SEQ ID NO: 5740; SEQ ID NO: 5741; SEQ ID NO: 5742; SEQ ID NO: 5743; SEQ ID NO: 5744; SEQ ID NO: 5745; SEQ ID NO: 5746; SEQ ID NO: 5747; SEQ ID NO: 5748; SEQ ID NO: 5749; SEQ ID NO: 5750; SEQ ID NO: 5751; SEQ ID NO: 5752; SEQ ID NO: 5753; SEQ ID NO: 5754; SEQ ID NO: 5755; SEQ ID NO: 5756; SEQ ID NO: 5757; SEQ ID NO: 5758; SEQ ID NO: 5759; SEQ ID NO: 5760; SEQ ID NO: 5761; SEQ ID NO: 5762; SEQ ID NO: 5763; SEQ ID NO: 5764; SEQ ID NO: 5765; SEQ ID NO: 5766; SEQ ID NO: 5767; SEQ ID NO: 5768; SEQ ID NO: 5769; SEQ ID NO: 5770; SEQ ID NO: 5771; SEQ ID NO: 5772; SEQ ID NO: 5773; SEQ ID NO: 5774; SEQ ID NO: 5775; SEQ ID NO: 5776; SEQ ID NO: 5777; SEQ ID NO: 5778; SEQ ID NO: 5779; SEQ ID NO: 5780; SEQ ID NO: 5781; SEQ ID NO: 5782; SEQ ID NO: 5783; SEQ ID NO: 5784; SEQ ID NO: 5785; SEQ ID NO: 5786; SEQ ID NO: 5787; SEQ ID NO: 5788; SEQ ID NO: 5789; SEQ ID NO: 5790; SEQ ID NO: 5791; SEQ ID NO: 5792; SEQ ID NO: 5793; SEQ ID NO: 5794; SEQ ID NO: 5795; SEQ ID NO: 5796; SEQ ID NO: 5797; SEQ ID NO: 5798; SEQ ID NO: 5799; SEQ ID NO: 5800; SEQ ID NO: 5801; SEQ ID NO: 5802; SEQ ID NO: 5803; SEQ ID NO: 5804; SEQ ID NO: 5805; SEQ ID NO: 5806; SEQ ID NO: 5807; SEQ ID NO: 5808; SEQ ID NO: 5809; SEQ ID NO: 5810; SEQ ID NO: 5811; SEQ ID NO: 5812; SEQ ID NO: 5813; SEQ ID NO: 5814; SEQ ID NO: 5815; SEQ ID NO: 5816; SEQ ID NO: 5817; SEQ ID NO: 5818; SEQ ID NO: 5819; SEQ ID NO: 5820; SEQ ID NO: 5821; SEQ ID NO: 5822; SEQ ID NO: 5823; SEQ ID NO: 5824; SEQ ID NO: 5825; SEQ ID NO: 5826; SEQ ID NO: 5827; SEQ ID NO: 5828; SEQ ID NO: 5829; SEQ ID NO: 5830; SEQ ID NO: 5831; SEQ ID NO: 5832; SEQ ID NO: 5833; SEQ ID NO: 5834; SEQ ID NO: 5835; SEQ ID NO: 5836; SEQ ID NO: 5837; SEQ ID NO: 5838; SEQ ID NO: 5839; SEQ ID NO: 5840; SEQ ID NO: 5841; SEQ ID NO: 5842; SEQ ID NO: 5843; SEQ ID NO: 5844; SEQ ID NO: 5845; SEQ ID NO: 5846; SEQ ID NO: 5847; SEQ ID NO: 5848; SEQ ID NO: 5849; SEQ ID NO: 5850; SEQ ID NO: 5851; SEQ ID NO: 5852; SEQ ID NO: 5853; SEQ ID NO: 5854; SEQ ID NO: 5855; SEQ ID NO: 5856; SEQ ID NO: 5857; SEQ ID NO: 5858; SEQ ID NO: 5859; SEQ ID NO: 5860; SEQ ID NO: 5861; SEQ ID NO: 5862; SEQ ID NO: 5863; SEQ ID NO: 5864; SEQ ID NO: 5865; SEQ ID NO: 5866; SEQ ID NO: 5867; SEQ ID NO: 5868; SEQ ID NO: 5869; SEQ ID NO: 5870; SEQ ID NO: 5871; SEQ ID NO: 5872; SEQ ID NO: 5873; SEQ ID NO: 5874; SEQ ID NO: 5875; SEQ ID NO: 5876; SEQ ID NO: 5877; SEQ ID NO: 5878; SEQ ID NO: 5879; SEQ ID NO: 5880; SEQ ID NO: 5881; SEQ ID NO: 5882; SEQ ID NO: 5883; SEQ ID NO: 5884; SEQ ID NO: 5885; SEQ ID NO: 5886; SEQ ID NO: 5887; SEQ ID NO: 5888; SEQ ID NO: 5889; SEQ ID NO: 5890; SEQ ID NO: 5891; SEQ ID NO: 5892; SEQ ID NO: 5893; SEQ ID NO: 5894; SEQ ID NO: 5895; SEQ ID NO: 5896; SEQ ID NO: 5897; SEQ ID NO: 5898; SEQ ID NO: 5899; SEQ ID NO: 5900; SEQ ID NO: 5901; SEQ ID NO: 5902; SEQ ID NO: 5903; SEQ ID NO: 5904; SEQ ID NO: 5905; SEQ ID NO: 5906; SEQ ID NO: 5907; SEQ ID NO: 5908; SEQ ID NO: 5909; SEQ ID NO: 5910; SEQ ID NO: 5911; SEQ ID NO: 5912; SEQ ID NO: 5913; SEQ ID NO: 5914; SEQ ID NO: 5915; SEQ ID NO: 5916; SEQ ID NO: 5917; SEQ ID NO: 5918; SEQ ID NO: 5919; SEQ ID NO: 5920; SEQ ID NO: 5921; SEQ ID NO: 5922; SEQ ID NO: 5923; SEQ ID NO: 5924; SEQ ID NO: 5925; SEQ ID NO: 5926; SEQ ID NO: 5927; SEQ ID NO: 5928; SEQ ID NO: 5929; SEQ ID NO: 5930; SEQ ID NO: 5931; SEQ ID NO: 5932; SEQ ID NO: 5933; SEQ ID NO: 5934; SEQ ID NO: 5935; SEQ ID NO: 5936; SEQ ID NO: 5937; SEQ ID NO: 5938; SEQ ID NO: 5939; SEQ ID NO: 5940; SEQ ID NO: 5941; SEQ ID NO: 5942; SEQ ID NO: 5943; SEQ ID NO: 5944; SEQ ID NO: 5945; SEQ ID NO: 5946; SEQ ID NO: 5947; SEQ ID NO: 5948; SEQ ID NO: 5949; SEQ ID NO: 5950; SEQ ID NO: 5951; SEQ ID NO: 5952; SEQ ID NO: 5953; SEQ ID NO: 5954; SEQ ID NO: 5955; SEQ ID NO: 5956; SEQ ID NO: 5957; SEQ ID NO: 5958; SEQ ID NO: 5959; SEQ ID NO: 5960; SEQ ID NO: 5961; SEQ ID NO: 5962; SEQ ID NO: 5963; SEQ ID NO: 5964; SEQ ID NO: 5965; SEQ ID NO: 5966; SEQ ID NO: 5967; SEQ ID NO: 5968; SEQ ID NO: 5969; SEQ ID NO: 5970; SEQ ID NO: 5971; SEQ ID NO: 5972; SEQ ID NO: 5973; SEQ ID NO: 5974; SEQ ID NO: 5975; SEQ ID NO: 5976; SEQ ID NO: 5977; SEQ ID NO: 5978; SEQ ID NO: 5979; SEQ ID NO: 5980; SEQ ID NO: 5981; SEQ ID NO: 5982; SEQ ID NO: 5983; SEQ ID NO: 5984; SEQ ID NO: 5985; SEQ ID NO: 5986; SEQ ID NO: 5987; SEQ ID NO: 5988; SEQ ID NO: 5989; SEQ ID NO: 5990; SEQ ID NO: 5991; SEQ ID NO: 5992; SEQ ID NO: 5993; SEQ ID NO: 5994; SEQ ID NO: 5995; SEQ ID NO: 5996; SEQ ID NO: 5997; SEQ ID NO: 5998; SEQ ID NO: 5999; SEQ ID NO: 6000; SEQ ID NO: 6001; SEQ ID NO: 6002; SEQ ID NO: 6003; SEQ ID NO: 6004; SEQ ID NO: 6005; SEQ ID NO: 6006; SEQ ID NO: 6007; SEQ ID NO: 6008; SEQ ID NO: 6009; SEQ ID NO: 6010; SEQ ID NO: 6011; SEQ ID NO: 6012; SEQ ID NO: 6013; SEQ ID NO: 6014; SEQ ID NO: 6015; SEQ ID NO: 6016; SEQ ID NO: 6017; SEQ ID NO: 6018; SEQ ID NO: 6019; SEQ ID NO: 6020; SEQ ID NO: 6021; SEQ ID NO: 6022; SEQ ID NO: 6023; SEQ ID NO: 6024; SEQ ID NO: 6025; SEQ ID NO: 6026; SEQ ID NO: 6027; SEQ ID NO: 6028; SEQ ID NO: 6029; SEQ ID NO: 6030; SEQ ID NO: 6031; SEQ ID NO: 6032; SEQ ID NO: 6033; SEQ ID NO: 6034; SEQ ID NO: 6035; SEQ ID NO: 6036; SEQ ID NO: 6037; SEQ ID NO: 6038; SEQ ID NO: 6039; SEQ ID NO: 6040; SEQ ID NO: 6041; SEQ ID NO: 6042; SEQ ID NO: 6043; SEQ ID NO: 6044; SEQ ID NO: 6045; SEQ ID NO: 6046; SEQ TD NO: 6047; SEQ ID NO: 6048; SEQ ID NO: 6049; SEQ ID NO: 6050; SEQ ID NO: 6051; SEQ ID NO: 6052; SEQ ID NO: 6053; SEQ ID NO: 6054; SEQ ID NO: 6055; SEQ ID NO: 6056; SEQ ID NO: 6057; SEQ ID NO: 6058; SEQ ID NO: 6059; SEQ ID NO: 6060; SEQ ID NO: 6061; SEQ ID NO: 6062; SEQ ID NO: 6063; SEQ ID NO: 6064; SEQ ID NO: 6065; SEQ ID NO: 6066; SEQ ID NO: 6067; SEQ ID NO: 6068; SEQ ID NO: 6069; SEQ ID NO: 6070; SEQ ID NO: 6071; SEQ ID NO: 6072; SEQ ID NO: 6073; SEQ ID NO: 6074; SEQ ID NO: 6075; SEQ ID NO: 6076; SEQ ID NO: 6077; SEQ ID NO: 6078; SEQ ID NO: 6079; SEQ ID NO: 6080; SEQ ID NO: 6081; SEQ ID NO: 6082; SEQ ID NO: 6083; SEQ ID NO: 6084; SEQ ID NO: 6085; SEQ ID NO: 6086; SEQ ID NO: 6087; SEQ ID NO: 6088; SEQ ID NO: 6089; SEQ ID NO: 6090; SEQ ID NO: 6091; SEQ ID NO: 6092; SEQ ID NO: 6093; SEQ ID NO: 6094; SEQ ID NO: 6095; SEQ ID NO: 6096; SEQ ID NO: 6097; SEQ ID NO: 6098; SEQ ID NO: 6099; SEQ ID NO: 6100; SEQ ID NO: 6101; SEQ ID NO: 6102; SEQ ID NO: 6103; SEQ ID NO: 6104; SEQ ID NO: 6105; SEQ ID NO: 6106; SEQ ID NO: 6107; SEQ ID NO: 6108; SEQ ID NO: 6109; SEQ ID NO: 6110; SEQ ID NO: 6111; SEQ ID NO: 6112; SEQ ID NO: 6113; SEQ ID NO: 6114; SEQ ID NO: 6115; SEQ ID NO: 6116; SEQ ID NO: 6117; SEQ ID NO: 6118; SEQ ID NO: 6119; SEQ ID NO: 6120; SEQ ID NO: 6121; SEQ ID NO: 6122; SEQ ID NO: 6123; SEQ ID NO: 6124; SEQ ID NO: 6125; SEQ ID NO: 6126; SEQ ID NO: 6127; SEQ ID NO: 6128; SEQ ID NO: 6129; SEQ ID NO: 6130; SEQ ID NO: 6131; SEQ ID NO: 6132; SEQ ID NO: 6133; SEQ ID NO: 6134; SEQ ID NO: 6135; SEQ ID NO: 6136; SEQ ID NO: 6137; SEQ ID NO: 6138; SEQ ID NO: 6139; SEQ ID NO: 6140; SEQ ID NO: 6141; SEQ ID NO: 6142; SEQ ID NO: 6143; SEQ ID NO: 6144; SEQ ID NO: 6145; SEQ ID NO: 6146; SEQ ID NO: 6147; SEQ ID NO: 6148; SEQ ID NO: 6149; SEQ ID NO: 6150; SEQ ID NO: 6151; SEQ ID NO: 6152; SEQ ID NO: 6153; SEQ ID NO: 6154; SEQ ID NO: 6155; SEQ ID NO: 6156; SEQ ID NO: 6157; SEQ ID NO: 6158; SEQ ID NO: 6159; SEQ ID NO: 6160; SEQ ID NO: 6161; SEQ ID NO: 6162; SEQ ID NO: 6163; SEQ ID NO: 6164; SEQ ID NO: 6165; SEQ ID NO: 6166; SEQ ID NO: 6167; SEQ ID NO: 6168; SEQ ID NO: 6169; SEQ ID NO: 6170; SEQ ID NO: 6171; SEQ ID NO: 6172; SEQ ID NO: 6173; SEQ ID NO: 6174; SEQ ID NO: 6175; SEQ ID NO: 6176; SEQ ID NO: 6177; SEQ ID NO: 6178; SEQ ID NO: 6179; SEQ ID NO: 6180; SEQ ID NO: 6181; SEQ ID NO: 6182; SEQ ID NO: 6183; SEQ ID NO: 6184; SEQ ID NO: 6185; SEQ ID NO: 6186; SEQ ID NO: 6187; SEQ ID NO: 6188; SEQ ID NO: 6189; SEQ ID NO: 6190; SEQ ID NO: 6191; SEQ ID NO: 6192; SEQ ID NO: 6193; SEQ ID NO: 6194; SEQ ID NO: 6195; SEQ ID NO: 6196; SEQ ID NO: 6197; SEQ ID NO: 6198; SEQ ID NO: 6199; SEQ ID NO: 6200; SEQ ID NO: 6201; SEQ ID NO: 6202; SEQ ID NO: 6203; SEQ ID NO: 6204; SEQ ID NO: 6205; SEQ ID NO: 6206; SEQ ID NO: 6207; SEQ ID NO: 6208; SEQ ID NO: 6209; SEQ ID NO: 6210; SEQ ID NO: 6211; SEQ ID NO: 6212; SEQ ID NO: 6213; SEQ ID NO: 6214; SEQ ID NO: 6215; SEQ ID NO: 6216; SEQ ID NO: 6217; SEQ ID NO: 6218; SEQ ID NO: 6219; SEQ ID NO: 6220; SEQ ID NO: 6221; SEQ ID NO: 6222; SEQ ID NO: 6223; SEQ ID NO: 6224; SEQ ID NO: 6225; SEQ ID NO: 6226; SEQ ID NO: 6227; SEQ ID NO: 6228; SEQ ID NO: 6229; SEQ ID NO: 6230; SEQ ID NO: 6231; SEQ ID NO: 6232; SEQ ID NO: 6233; SEQ ID NO: 6234; SEQ ID NO: 6235; SEQ ID NO: 6236; SEQ ID NO: 6237; SEQ ID NO: 6238; SEQ ID NO: 6239; SEQ ID NO: 6240; SEQ ID NO: 6241; SEQ ID NO: 6242; SEQ ID NO: 6243; SEQ ID NO: 6244; SEQ ID NO: 6245; SEQ ID NO: 6246; SEQ ID NO: 6247; SEQ ID NO: 6248; SEQ ID NO: 6249; SEQ ID NO: 6250; SEQ ID NO: 6251; SEQ ID NO: 6252; SEQ ID NO: 6253; SEQ ID NO: 6254; SEQ ID NO: 6255; SEQ ID NO: 6256; SEQ ID NO: 6257; SEQ ID NO: 6258; SEQ ID NO: 6259; SEQ ID NO: 6260; SEQ ID NO: 6261; SEQ ID NO: 6262; SEQ ID NO: 6263; SEQ ID NO: 6264; SEQ ID NO: 6265; SEQ ID NO: 6266; SEQ ID NO: 6267; SEQ ID NO: 6268; SEQ ID NO: 6269; SEQ ID NO: 6270; SEQ ID NO: 6271; SEQ ID NO: 6272; SEQ ID NO: 6273; SEQ ID NO: 6274; SEQ ID NO: 6275; SEQ ID NO: 6276; SEQ ID NO: 9649; SEQ ID NO: 9650; SEQ ID NO: 9651; SEQ ID NO: 9652; SEQ ID NO: 9653; SEQ ID NO: 9654; SEQ ID NO: 9655; SEQ ID NO: 9656; SEQ ID NO: 9657; SEQ ID NO: 9658; SEQ ID NO: 9659; SEQ ID NO: 9660; SEQ ID NO: 9661; SEQ ID NO: 9662; SEQ ID NO: 9663; SEQ ID NO: 9664; SEQ ID NO: 9665; SEQ ID NO: 9666; SEQ ID NO: 9667; SEQ ID NO: 9668; SEQ ID NO: 9669; SEQ ID NO: 9670; SEQ ID NO: 9671; SEQ ID NO: 9672; SEQ ID NO: 9673; SEQ ID NO: 9674; SEQ ID NO: 9675; SEQ ID NO: 9676; SEQ ID NO: 9677; SEQ ID NO: 9678; SEQ ID NO: 9679; SEQ ID NO: 9680; SEQ ID NO: 9681; SEQ ID NO: 9682; SEQ ID NO: 9683; SEQ ID NO: 9684; SEQ ID NO: 9685; SEQ ID NO: 9686; SEQ ID NO: 9687; SEQ ID NO: 9688; SEQ ID NO: 9689; SEQ ID NO: 9690; SEQ ID NO: 9691; SEQ ID NO: 9692; SEQ ID NO: 9693; SEQ ID NO: 9694; SEQ ID NO: 9695; SEQ ID NO: 9696; SEQ ID NO: 9697; SEQ ID NO: 9698; SEQ ID NO: 9699; SEQ ID NO: 9700; SEQ ID NO: 9701; SEQ ID NO: 9702 and SEQ ID NO: 9703.  
     [0104] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in transport having an amino acid sequence selected from the group consisting of SEQ ID NO: 5273; SEQ ID NO: 5274; SEQ ID NO: 5275; SEQ ID NO: 5276; SEQ ID NO: 5277; SEQ ID NO: 5278; SEQ ID NO: 5279; SEQ ID NO: 5280; SEQ ID NO: 5281; SEQ ID NO: 5282; SEQ ID NO: 5283; SEQ ID NO: 5284; SEQ ID NO: 5285; SEQ ID NO: 5286; SEQ ID NO: 5287; SEQ ID NO: 5288; SEQ ID NO: 5289; SEQ ID NO: 5290; SEQ ID NO: 5291; SEQ ID NO: 5292; SEQ ID NO: 5293; SEQ ID NO: 5294; SEQ ID NO: 5295; SEQ ID NO: 5296; SEQ ID NO: 5297; SEQ ID NO: 5298; SEQ ID NO: 5299; SEQ ID NO: 5300; SEQ ID NO: 5301; SEQ ID NO: 5302; SEQ ID NO: 5303; SEQ ID NO: 5304; SEQ ID NO: 5305; SEQ ID NO: 5306; SEQ ID NO: 5307; SEQ ID NO: 5308; SEQ ID NO: 5309; SEQ ID NO: 5310; SEQ ID NO: 5311; SEQ ID NO: 5312; SEQ ID NO: 5313; SEQ ID NO: 5314; SEQ ID NO: 5315; SEQ ID NO: 5316; SEQ ID NO: 5317; SEQ ID NO: 5318; SEQ ID NO: 5319; SEQ ID NO: 5320; SEQ ID NO: 5321; SEQ ID NO: 5322; SEQ ID NO: 5323; SEQ ID NO: 5324; SEQ ID NO: 5325; SEQ ID NO: 5326; SEQ ID NO: 5327; SEQ ID NO: 5328; SEQ ID NO: 5329; SEQ ID NO: 5330; SEQ ID NO:5331; SEQ ID NO: 5332; SEQ ID NO: 5333; SEQ ID NO: 5334; SEQ ID NO: 5335; SEQ ID NO: 5336; SEQ ID NO: 5337; SEQ ID NO: 5338; SEQ ID NO: 5339; SEQ ID NO: 5340; SEQ ID NO: 5341; SEQ ID NO: 5342; SEQ ID NO: 5343; SEQ ID NO: 5344; SEQ ID NO: 5345; SEQ ID NO: 5346; SEQ ID NO: 5347; SEQ ID NO: 5348; SEQ ID NO: 5349; SEQ ID NO: 5350; SEQ ID NO: 5351; SEQ ID NO: 5352; SEQ ID NO: 5353; SEQ ID NO: 5354; SEQ ID NO: 5355; SEQ ID NO: 5356; SEQ ID NO: 5357; SEQ ID NO: 5358; SEQ ID NO: 5359; SEQ ID NO: 5360; SEQ ID NO: 5361; SEQ ID NO: 5362; SEQ ID NO: 5363; SEQ ID NO: 5364; SEQ ID NO: 5365; SEQ ID NO: 5366; SEQ ID NO: 5367; SEQ ID NO: 5368; SEQ ID NO: 5369; SEQ ID NO: 5370; SEQ ID NO: 5371; SEQ ID NO: 5372; SEQ ID NO: 5373; SEQ ID NO: 5374; SEQ ID NO: 5375; SEQ ID NO: 5376; SEQ ID NO: 5377; SEQ ID NO: 5378; SEQ ID NO: 5379; SEQ ID NO: 5380; SEQ ID NO: 5381; SEQ ID NO: 5382; SEQ ID NO: 5383; SEQ ID NO: 5384; SEQ ID NO: 5385; SEQ ID NO: 5386; SEQ ID NO: 5387; SEQ ID NO: 5388; SEQ ID NO: 5389; SEQ ID NO: 5390; SEQ ID NO: 5391; SEQ ID NO: 5392; SEQ ID NO: 5393; SEQ ID NO: 5394; SEQ ID NO: 5395; SEQ ID NO: 5396; SEQ ID NO: 5397; SEQ ID NO: 5398; SEQ ID NO: 5399; SEQ ID NO: 5400; SEQ ID NO: 5401; SEQ ID NO: 5402; SEQ ID NO: 5403; SEQ ID NO: 5404; SEQ ID NO: 5405; SEQ ID NO: 5406; SEQ ID NO: 5407; SEQ ID NO: 5408; SEQ ID NO: 5409; SEQ ID NO: 5410; SEQ ID NO: 5411; SEQ ID NO: 5412; SEQ ID NO: 5413; SEQ ID NO: 5414; SEQ ID NO: 5415; SEQ ID NO: 5416; SEQ ID NO: 5417; SEQ ID NO: 5418; SEQ ID NO: 5419; SEQ ID NO: 5420; SEQ ID NO: 5421; SEQ ID NO: 5422; SEQ ID NO: 5423; SEQ ID NO: 5424; SEQ ID NO: 5425; SEQ ID NO: 5426; SEQ ID NO: 5427; SEQ ID NO: 5428; SEQ ID NO:5429; SEQ ID NO: 5430; SEQ ID NO:5431; SEQ ID NO: 5432; SEQ ID NO: 5433; SEQ ID NO:5434; SEQ ID NO: 5435; SEQ ID NO: 5436; SEQ ID NO: 5437; SEQ ID NO: 5438; SEQ ID NO: 5439; SEQ ID NO: 5440; SEQ ID NO: 5441; SEQ ID NO: 5442; SEQ ID NO: 5443; SEQ ID NO: 5444; SEQ ID NO: 5564; SEQ ID NO: 9649; SEQ ID NO: 9650; SEQ ID NO: 9651; SEQ ID NO: 9652; SEQ ID NO: 9653; SEQ ID NO: 9654; SEQ ID NO: 9655; SEQ ID NO: 9656; SEQ ID NO: 9657; SEQ ID NO: 9658; SEQ ID NO: 9659; SEQ ID NO: 9660; SEQ ID NO: 9661; SEQ ID NO: 9662; SEQ ID NO: 9663; SEQ ID NO: 9664; SEQ ID NO: 9665 and SEQ ID NO: 9666.  
     [0105] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in amino acid metabolism and transport having an amino acid sequence selected from the group consisting of SEQ ID NO: 5445; SEQ ID NO: 5446; SEQ ID NO: 5447; SEQ ID NO: 5448; SEQ ID NO: 5449; SEQ ID NO: 5450; SEQ ID NO: 5451; SEQ ID NO: 5452; SEQ ID NO: 5453; SEQ ID NO: 5454; SEQ ID NO: 5455; SEQ ID NO: 5456; SEQ ID NO: 5457; SEQ ID NO: 5458; SEQ ID NO: 5459; SEQ ID NO: 5460; SEQ ID NO: 5461; SEQ ID NO: 5462; SEQ ID NO: 5463; SEQ ID NO: 5464; SEQ ID NO: 5465; SEQ ID NO: 5466; SEQ ID NO: 5467; SEQ ID NO: 5468; SEQ ID NO: 5469; SEQ ID NO: 5470; SEQ ID NO: 5471; SEQ ID NO: 5472; SEQ ID NO: 5473; SEQ ID NO: 5474; SEQ ID NO: 5475; SEQ ID NO: 5476; SEQ ID NO: 5477; SEQ ID NO: 5478; SEQ ID NO: 5479; SEQ ID NO: 5480; SEQ ID NO: 5481; SEQ ID NO: 5482; SEQ ID NO: 9659 and SEQ ID NO: 9660.  
     [0106] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in nucleotide, lipid, or cofactor metabolism and transport having an amino acid sequence selected from the group consisting of SEQ ID NO: 5483; SEQ ID NO: 5484; SEQ ID NO: 5485; SEQ ID NO: 5486; SEQ ID NO: 5487; SEQ ID NO: 5488; SEQ ID NO: 5489; SEQ ID NO: 5490; SEQ ID NO: 5491; SEQ ID NO: 5492; SEQ ID NO: 5493; SEQ ID NO: 5494; SEQ ID NO: 5495; SEQ ID NO: 5496; SEQ ID NO: 5497; SEQ ID NO: 5498; SEQ ID NO: 5499; SEQ ID NO: 5500; SEQ ID NO: 5501; SEQ ID NO: 5502; SEQ ID NO: 5503; SEQ ID NO: 5504; SEQ ID NO: 5505; SEQ ID NO: 5506; SEQ ID NO: 5507; SEQ ID NO: 5508; SEQ ID NO: 5509; SEQ ID NO: 5510; SEQ ID NO: 5511; SEQ ID NO: 5512; SEQ ID NO: 5513; SEQ ID NO: 5514; SEQ ID NO: 5515; SEQ ID NO: 5516; SEQ ID NO: 5517; SEQ ID NO: 5518; SEQ ID NO: 5519; SEQ ID NO: 5520; SEQ ID NO: 5521; SEQ ID NO: 5522; SEQ ID NO: 5523; SEQ ID NO: 5524; SEQ ID NO: 5525; SEQ ID NO: 5526 and SEQ ID NO: 9661.  
     [0107] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in inorganic ion transport having an amino acid sequence selected from the group consisting of SEQ ID NO: 5527; SEQ ID NO: 5528; SEQ ID NO: 5529; SEQ ID NO: 5530; SEQ ID NO: 5531; SEQ ID NO: 5532; SEQ ID NO: 5533; SEQ ID NO: 5534; SEQ ID NO: 5535; SEQ ID NO: 5536; SEQ ID NO: 5537; SEQ ID NO: 5538; SEQ ID NO: 5539; SEQ ID NO: 5540; SEQ ID NO: 5541; SEQ ID NO: 5542; SEQ ID NO: 5543; SEQ ID NO: 5544; SEQ ID NO: 5545; SEQ ID NO: 5546; SEQ ID NO: 5547; SEQ ID NO: 5548; SEQ ID NO: 5549; SEQ ID NO: 5550; SEQ ID NO: 5551; SEQ ID NO: 5552; SEQ ID NO: 5553; SEQ ID NO: 5554; SEQ ID NO: 5555; SEQ ID NO: 5556; SEQ ID NO: 5557; SEQ ID NO: 5558; SEQ ID NO: 5559; SEQ ID NO: 5560; SEQ ID NO: 5561; SEQ ID NO: 9662; SEQ ID NO: 9663; SEQ ID NO: 9664; SEQ ID NO: 9665 and SEQ ID NO: 9666.  
     [0108] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in carbohydrate metabolism and transport having an amino acid sequence selected from the group consisting of SEQ ID NO: 5562; SEQ ID NO: 5563 and SEQ ID NO: 5564.  
     [0109] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in outer membrane and cell wall formation having an amino acid sequence selected from the group consisting of SEQ ID NO: 5565; SEQ ID NO: 5566; SEQ ID NO: 5567; SEQ ID NO: 5568; SEQ ID NO: 5569; SEQ ID NO: 5570; SEQ ID NO: 5571; SEQ ID NO: 5572; SEQ ID NO: 5573; SEQ ID NO: 5574; SEQ ID NO: 5575; SEQ ID NO: 5576; SEQ ID NO: 5577; SEQ ID NO: 5578; SEQ ID NO: 5579; SEQ ID NO: 5580; SEQ ID NO: 5581; SEQ ID NO: 5582; SEQ ID NO: 5583; SEQ ID NO: 5584; SEQ ID NO: 5585; SEQ ID NO: 5586; SEQ ID NO: 5587; SEQ ID NO: 5588; SEQ ID NO: 5589; SEQ ID NO: 5590; SEQ ID NO: 5591; SEQ ID NO: 5592; SEQ ID NO: 5593; SEQ ID NO: 5594; SEQ ID NO: 5595; SEQ ID NO: 5596; SEQ ID NO: 5597; SEQ ID NO: 5598; SEQ ID NO: 5599; SEQ ID NO: 5600; SEQ ID NO: 5601; SEQ ID NO: 5602; SEQ ID NO: 5603; SEQ ID NO: 5604; SEQ ID NO: 5605; SEQ ID NO: 5606; SEQ ID NO: 5607; SEQ ID NO: 5608; SEQ ID NO: 5609; SEQ ID NO: 5610; SEQ ID NO: 9667; SEQ ID NO: 9668; SEQ ID NO: 9669; SEQ ID NO: 9670; SEQ ID NO: 9671 and SEQ ID NO: 9672.  
     [0110] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in energy conversion having an amino acid sequence selected from the group consisting of SEQ ID NO: 5611; SEQ ID NO: 5612; SEQ ID NO: 5613; SEQ ID NO: 5614; SEQ ID NO: 5615; SEQ ID NO: 5616; SEQ ID NO: 5617; SEQ ID NO: 5618; SEQ ID NO: 5619; SEQ ID NO: 5620; SEQ ID NO: 5621; SEQ ID NO: 5622; SEQ ID NO: 5623; SEQ ID NO: 5624; SEQ ID NO: 5625; SEQ ID NO: 5626; SEQ ID NO: 5627; SEQ ID NO: 5628; SEQ ID NO: 5629; SEQ ID NO: 5630; SEQ ID NO: 5631; SEQ ID NO: 5632; SEQ ID NO: 5633; SEQ ID NO: 5634; SEQ ID NO: 5635; SEQ ID NO: 5636; SEQ ID NO: 5637; SEQ ID NO: 5638; SEQ ID NO: 5639; SEQ ID NO: 5640; SEQ ID NO: 5641; SEQ ID NO: 5642; SEQ ID NO: 5643; SEQ ID NO: 5644; SEQ ID NO: 5645; SEQ ID NO: 5646; SEQ ID NO: 5647; SEQ ID NO: 5648; SEQ ID NO: 5649; SEQ ID NO: 5650; SEQ ID NO: 5651; SEQ ID NO: 5652; SEQ ID NO:5653; SEQ ID NO: 5654; SEQ ID NO: 5655; SEQ ID NO: 5656; SEQ ID NO: 5657; SEQ ID NO: 5658; SEQ ID NO: 5659; SEQ ID NO: 5660; SEQ ID NO: 5661; SEQ ID NO: 5662; SEQ ID NO: 5663; SEQ ID NO: 5664; SEQ ID NO: 5665; SEQ ID NO: 5666; SEQ ID NO: 5667; SEQ ID NO: 5668; SEQ ID NO: 5669; SEQ ID NO: 5670; SEQ ID NO: 5671; SEQ ID NO: 5672; SEQ ID NO: 5673; SEQ ID NO: 5674; StQ ID NO: 5675; SEQ ID NO: 5676; SEQ ID NO: 5677; SEQ ID NO: 5678; SEQ ID NO: 5679; SEQ ID NO: 5680; SEQ ID NO: 5681; SEQ ID NO:5682; SEQ ID NO: 5683; SEQ ID NO: 5684; SEQ ID NO: 5685; SEQ ID NO: 5686; SEQ ID NO: 5687; SEQ ID NO: 5688; SEQ ID NO: 5689; SEQ ID NO: 5690; SEQ ID NO: 5691; SEQ ID NO: 5692; SEQ ID NO: 5693; SEQ ID NO: 5694; SEQ ID NO: 5695; SEQ ID NO: 5696; SEQ ID NO: 5697; SEQ ID NO: 5698; SEQ ID NO: 5699; SEQ ID NO: 5700; SEQ ID NO: 5701; SEQ ID NO: 5702; SEQ ID NO: 5703; SEQ ID NO: 5704; SEQ ID NO: 5705; SEQ ID NO: 5706; SEQ ID NO: 5707; SEQ ID NO: 5708; SEQ ID NO: 5709; SEQ ID NO: 5710; SEQ ID NO: 5711; SEQ ID NO: 5712; SEQ ID NO: 5713; SEQ ID NO: 5714; SEQ ID NO: 5715; SEQ ID NO: 5716; SEQ ID NO: 5717; SEQ ID NO: 5718; SEQ ID NO: 5719; SEQ ID NO: 5720; SEQ ID NO: 5721; SEQ ID NO: 5722; SEQ ID NO: 5723; SEQ ID NO: 5724; SEQ ID NO: 5725; SEQ ID NO: 5726; SEQ ID NO: 5727; SEQ ID NO: 5728; SEQ ID NO: 5729; SEQ ID NO: 5730; SEQ ID NO: 5731; SEQ ID NO: 5732; SEQ ID NO: 5733; SEQ ID NO: 5734; SEQ ID NO: 5735; SEQ ID NO: 5736; SEQ ID NO: 5737; SEQ ID NO: 5738; SEQ ID NO: 5739; SEQ ID NO: 5740; SEQ ID NO: 5741; SEQ ID NO: 5742; SEQ ID NO: 5743; SEQ ID NO: 5744; SEQ ID NO: 5745; SEQ ID NO: 5746; SEQ ID NO: 5747; SEQ ID NO: 5748; SEQ ID NO: 5749; SEQ ID NO: 5750; SEQ ID NO: 5751; SEQ ID NO: 5752; SEQ ID NO: 5753; SEQ ID NO: 5754; SEQ ID NO: 5755; SEQ ID NO: 5756; SEQ ID NO: 5757; SEQ ID NO: 9673; SEQ ID NO: 9674; SEQ ID NO: 9675; SEQ ID NO: 9676; SEQ ID NO: 9677.  
     [0111] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in secretion and adhesion having an amino acid sequence selected from the group consisting of SEQ ID NO: 5758; SEQ ID NO: 5759; SEQ ID NO: 5760; SEQ ID NO: 5761; SEQ ID NO: 5762; SEQ ID NO: 5763; SEQ ID NO: 5764; SEQ ID NO: 5765; SEQ ID NO: 5766; SEQ ID NO: 5767; SEQ ID NO: 5768; SEQ ID NO: 5769; SEQ ID NO: 9678.  
     [0112] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in regulation having an amino acid sequence selected from the group consisting of SEQ ID NO: 5770; SEQ ID NO: 5771; SEQ ID NO: 5772; SEQ ID NO: 5773; SEQ ID NO: 5774; SEQ ID NO: 5775; SEQ ID NO: 5776; SEQ ID NO: 5777; SEQ ID NO: 5778; SEQ ID NO: 5779; SEQ ID NO: 5780; SEQ ID NO: 5781; SEQ ID NO: 5782; SEQ ID NO: 5783; SEQ ID NO: 5784; SEQ ID NO: 5785; SEQ ID NO: 5786; SEQ ID NO: 5787; SEQ ID NO: 5788; SEQ ID NO: 5789.  
     [0113] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane chaperone polypeptide or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 5790; SEQ ID NO: 5791; SEQ ID NO: 5792; SEQ ID NO: 5793 and SEQ ID NO: 9679.  
     [0114] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in cell division having an amino acid sequence selected from the group consisting of SEQ ID NO: 5794; SEQ ID NO: 5795; SEQ ID NO: 5796; SEQ ID NO: 5797; SEQ ID NO: 5798; SEQ ID NO: 5799; SEQ ID NO: 5800; SEQ ID NO: 5801; SEQ ID NO: 5802; SEQ ID NO: 5803; SEQ ID NO: 5804; SEQ ID NO: 5805; SEQ ID NO: 5806; SEQ ID NO: 5807; SEQ ID NO: 5808; SEQ ID NO: 5809; SEQ ID NO: 5810; SEQ ID NO: 5811; SEQ ID NO: 5812; SEQ ID NO: 5813; SEQ ID NO: 5814; SEQ ID NO: 9680 and SEQ ID NO: 9681.  
     [0115] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in motility having an amino acid sequence selected from the group consisting of SEQ ID NO: 5815 and SEQ ID NO: 5816.  
     [0116] Particularly preferred is an isolated  H. pylori  cell envelope polypeptide or a fragment thereof, wherein the polypeptide is encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1; SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 5; SEQ ID NO: 6; SEQ ID NO: 7; SEQ ID NO: 8; SEQ ID NO: 9; SEQ ID NO: 10; SEQ ID NO:11; SEQ ID NO: 12; SEQ ID NO: 13; SEQ ID NO: 14; SEQ ID NO: 15; SEQ ID NO: 16; SEQ ID NO: 17; SEQ ID NO: 18; SEQ ID NO: 19; SEQ ID NO: 20; SEQ ID NO: 21; SEQ ID NO: 22; SEQ ID NO: 23; SEQ ID NO: 24; SEQ ID NO: 25; SEQ ID NO: 26; SEQ ID NO: 27; SEQ ID NO: 28; SEQ ID NO: 29; SEQ ID NO: 30; SEQ ID NO: 31; SEQ ID NO: 32; SEQ ID NO: 33; SEQ ID NO: 34; SEQ ID NO: 35; SEQ ID NO: 36; SEQ ID NO: 37; SEQ ID NO: 38; SEQ ID NO: 39; SEQ ID NO: 40; SEQ ID NO: 41; SEQ ID NO: 42; SEQ ID NO: 43; SEQ ID NO: 44; SEQ ID NO: 45; SEQ ID NO: 46; SEQ ID NO: 47; SEQ ID NO: 48; SEQ ID NO: 49; SEQ ID NO: 50; SEQ ID NO: 51; SEQ ID NO: 52; SEQ ID NO: 53; SEQ ID NO: 54; SEQ ID NO: 55; SEQ ID NO: 56; SEQ ID NO: 57; SEQ ID NO: 58; SEQ ID NO: 59; SEQ ID NO: 60; SEQ ID NO: 61; SEQ ID NO: 62; SEQ ID NO: 63; SEQ ID NO: 64; SEQ ID NO: 65; SEQ ID NO: 66; SEQ ID NO: 67; SEQ ID NO: 68; SEQ ID NO: 69; SEQ ID NO: 70; SEQ ID NO: 71; SEQ ID NO: 72; SEQ ID NO: 73; SEQ ID NO: 74; SEQ ID NO: 75; SEQ ID NO: 76; SEQ ID NO: 77; SEQ ID NO: 78; SEQ ID NO: 79; SEQ ID NO: 80; SEQ ID NO: 81; SEQ ID NO: 82; SEQ ID NO: 83; SEQ ID NO: 84; SEQ ID NO: 85; SEQ ID NO: 86; SEQ ID NO: 87; SEQ ID NO: 88; SEQ ID NO: 89; SEQ ID NO: 90; SEQ ID NO: 91; SEQ ID NO: 92; SEQ ID NO: 93; SEQ ID NO: 94; SEQ ID NO: 95; SEQ ID NO: 96; SEQ ID NO: 97; SEQ ID NO: 98; SEQ ID NO: 99; SEQ ID NO: 100; SEQ ID NO: 101; SEQ ID NO: 102; SEQ ID NO: 103; SEQ ID NO: 104; SEQ ID NO: 105; SEQ ID NO: 106; SEQ ID NO: 107; SEQ ID NO: 108; SEQ ID NO: 109; SEQ ID NO: 110; SEQ ID NO: 111; SEQ ID NO: 112; SEQ ID NO: 113; SEQ ID NO: 114; SEQ ID NO: 115; SEQ ID NO: 116; SEQ ID NO: 117; SEQ ID NO: 118; SEQ ID NO: 119; SEQ ID NO: 120; SEQ ID NO: 121; SEQ ID NO: 122; SEQ ID NO: 123; SEQ ID NO: 124; SEQ ID NO: 125; SEQ ID NO: 126; SEQ ID NO: 127; SEQ ID NO: 128; SEQ ID NO: 129; SEQ ID NO: 130; SEQ ID NO: 131; SEQ ID NO: 132; SEQ ID NO: 133; SEQ ID NO: 134; SEQ ID NO: 135; SEQ ID NO: 136; SEQ ID NO: 137; SEQ ID NO: 138; SEQ ID NO: 139; SEQ ID NO: 140; SEQ ID NO: 141; SEQ ID NO: 142; SEQ ID NO: 143; SEQ ID NO: 144; SEQ ID NO: 145; SEQ ID NO: 146; SEQ ID NO: 147; SEQ ID NO: 148; SEQ ID NO: 149; SEQ ID NO: 150; SEQ ID NO: 151; SEQ ID NO: 152; SEQ ID NO: 153; SEQ ID NO: 154; SEQ ID NO: 155; SEQ ID NO: 156; SEQ ID NO: 157; SEQ ID NO: 158; SEQ ID NO: 159; SEQ ID NO: 160; SEQ ID NO: 161; SEQ ID NO: 162; SEQ ID NO: 163; SEQ ID NO: 164; SEQ ID NO: 165; SEQ ID NO: 166; SEQ ID NO: 167; SEQ ID NO: 168; SEQ ID NO: 169; SEQ ID NO: 170; SEQ ID NO: 171; SEQ ID NO: 172; SEQ ID NO: 173; SEQ ID NO: 174; SEQ ID NO: 175; SEQ ID NO: 176; SEQ ID NO: 177; SEQ ID NO: 178; SEQ ID NO: 179; SEQ ID NO: 180; SEQ ID NO: 181; SEQ ID NO: 182; SEQ ID NO: 183; SEQ ID NO: 184; SEQ ID NO: 185; SEQ ID NO: 186; SEQ ID NO: 187; SEQ ID NO: 188; SEQ ID NO: 189; SEQ ID NO: 190; SEQ ID NO: 191; SEQ ID NO: 192; SEQ ID NO: 193; SEQ ID NO: 194; SEQ ID NO: 195; SEQ ID NO: 196; SEQ ID NO: 197; SEQ ID NO: 198; SEQ ID NO: 199; SEQ ID NO: 200; SEQ ID NO: 201; SEQ ID NO: 202; SEQ ID NO: 203; SEQ ID NO: 204; SEQ ID NO: 205; SEQ ID NO: 206; SEQ ID NO: 207; SEQ ID NO: 208; SEQ ID NO: 209; SEQ ID NO: 210; SEQ ID NO: 211; SEQ ID NO: 212; SEQ ID NO: 213; SEQ ID NO: 214; SEQ ID NO: 215; SEQ ID NO: 216; SEQ ID NO: 217; SEQ ID NO: 218; SEQ ID NO: 219; SEQ ID NO: 220; SEQ ID NO: 221; SEQ ID NO: 222; SEQ ID NO: 223; SEQ ID NO: 224; SEQ ID NO: 225; SEQ ID NO: 226; SEQ ID NO: 227; SEQ ID NO: 228; SEQ ID NO: 229; SEQ ID NO: 230; SEQ ID NO: 231; SEQ ID NO: 232; SEQ ID NO: 233; SEQ ID NO: 234; SEQ ID NO: 235; SEQ ID NO: 236; SEQ ID NO: 237; SEQ ID NO: 238; SEQ ID NO: 239; SEQ ID NO: 240; SEQ ID NO: 241; SEQ ID NO: 242; SEQ ID NO: 243; SEQ ID NO: 244; SEQ ID NO: 245; SEQ ID NO: 246; SEQ ID NO: 247; SEQ ID NO: 248; SEQ ID NO: 249; SEQ ID NO: 250; SEQ ID NO: 251; SEQ ID NO: 252; SEQ ID NO: 253; SEQ ID NO: 254; SEQ ID NO: 255; SEQ ID NO: 256; SEQ ID NO: 257; SEQ ID NO: 258; SEQ ID NO: 259; SEQ ID NO: 260; SEQ ID NO: 261; SEQ ID NO: 262; SEQ ID NO: 263; SEQ ID NO: 264; SEQ ID NO: 265; SEQ ID NO: 266; SEQ ID NO: 267; SEQ ID NO: 268; SEQ ID NO: 269; SEQ ID NO: 270; SEQ ID NO: 271; SEQ ID NO: 272; SEQ ID NO: 273; SEQ ID NO: 274; SEQ ID NO: 275; SEQ ID NO: 276; SEQ ID NO: 277; SEQ ID NO: 278; SEQ ID NO: 279; SEQ ID NO: 280; SEQ ID NO: 281; SEQ ID NO: 282; SEQ ID NO: 283; SEQ ID NO: 284; SEQ ID NO: 285; SEQ ID NO: 286; SEQ ID NO: 287; SEQ ID NO: 288; SEQ ID NO: 289; SEQ ID NO: 290; SEQ ID NO: 291; SEQ ID NO: 292; SEQ ID NO: 293; SEQ ID NO: 294; SEQ ID NO: 295; SEQ ID NO: 296; SEQ ID NO: 297; SEQ ID NO: 298; SEQ ID NO: 299; SEQ ID NO: 300; SEQ ID NO: 301; SEQ ID NO: 302; SEQ ID NO: 303; SEQ ID NO: 304; SEQ ID NO: 305; SEQ ID NO: 306; SEQ ID NO: 307; SEQ ID NO: 308; SEQ ID NO: 309; SEQ ID NO: 310; SEQ ID NO: 311; SEQ ID NO: 312; SEQ ID NO: 313; SEQ ID NO: 314; SEQ ID NO: 315; SEQ ID NO: 316; SEQ ID NO: 317; SEQ ID NO: 318; SEQ ID NO: 319; SEQ ID NO: 320; SEQ ID NO: 321; SEQ ID NO: 322; SEQ ID NO: 323; SEQ ID NO: 324; SEQ ID NO: 325; SEQ ID NO: 326; SEQ ID NO: 327; SEQ ID NO: 328; SEQ ID NO: 329; SEQ ID NO: 330; SEQ ID NO: 331; SEQ ID NO: 332; SEQ ID NO: 333; SEQ ID NO: 334; SEQ ID NO: 335; SEQ ID NO: 336; SEQ ID NO: 337; SEQ ID NO: 338; SEQ ID NO: 339; SEQ ID NO: 340; SEQ ID NO: 341; SEQ ID NO: 342; SEQ ID NO: 343; SEQ ID NO: 344; SEQ ID NO: 345; SEQ ID NO: 346; SEQ ID NO: 347; SEQ ID NO: 348; SEQ ID NO: 349; SEQ ID NO: 350; SEQ ID NO: 351; SEQ ID NO: 352; SEQ ID NO: 353; SEQ ID NO: 354; SEQ ID NO: 355; SEQ ID NO: 356; SEQ ID NO: 357; SEQ ID NO: 358; SEQ ID NO: 359; SEQ ID NO: 360; SEQ ID NO: 361; SEQ ID NO: 362; SEQ ID NO: 363; SEQ ID NO: 364; SEQ ID NO: 365; SEQ ID NO: 366; SEQ ID NO: 367; SEQ ID NO: 368; SEQ ID NO: 369; SEQ ID NO: 370; SEQ ID NO: 371; SEQ ID NO: 372; SEQ ID NO: 373; SEQ ID NO: 374; SEQ ID NO: 375; SEQ ID NO: 376; SEQ ID NO: 377; SEQ ID NO: 378; SEQ ID NO: 379; SEQ ID NO: 380; SEQ ID NO: 381; SEQ ID NO: 382; SEQ ID NO: 383; SEQ ID NO: 384; SEQ ID NO: 385; SEQ ID NO: 386; SEQ ID NO: 387; SEQ ID NO: 388; SEQ ID NO: 389; SEQ ID NO: 390; SEQ ID NO: 391; SEQ ID NO: 392; SEQ ID NO: 393; SEQ ID NO: 394; SEQ ID NO: 395; SEQ ID NO: 396; SEQ ID NO: 397; SEQ ID NO: 398; SEQ ID NO: 399; SEQ ID NO: 400; SEQ ID NO: 401; SEQ ID NO: 402; SEQ ID NO: 403; SEQ ID NO: 404; SEQ ID NO: 405; SEQ ID NO: 406; SEQ ID NO: 407; SEQ ID NO: 408; SEQ ID NO: 409; SEQ ID NO: 410; SEQ ID NO: 411; SEQ ID NO: 412; SEQ ID NO: 413; SEQ ID NO: 414; SEQ ID NO: 415; SEQ ID NO: 416; SEQ ID NO: 417; SEQ ID NO: 418; SEQ ID NO: 419; SEQ ID NO: 420; SEQ ID NO: 421; SEQ ID NO: 422; SEQ ID NO: 423; SEQ ID NO: 424; SEQ ID NO: 425; SEQ ID NO: 426; SEQ ID NO: 427; SEQ ID NO: 428; SEQ ID NO: 429; SEQ ID NO: 430; SEQ ID NO: 431; SEQ ID NO: 432; SEQ ID NO: 433; SEQ ID NO: 434; SEQ ID NO: 435; SEQ ID NO: 436; SEQ ID NO: 437; SEQ ID NO: 438; SEQ ID NO: 439; SEQ ID NO: 440; SEQ ID NO: 441; SEQ ID NO: 442; SEQ ID NO: 443; SEQ ID NO: 444; SEQ ID NO: 445; SEQ ID NO: 446; SEQ ID NO: 447; SEQ ID NO: 448; SEQ ID NO: 449; SEQ ID NO: 450; SEQ ID NO: 451; SEQ ID NO: 452; SEQ ID NO: 453; SEQ ID NO: 454; SEQ ID NO: 455; SEQ ID NO: 456; SEQ ID NO: 457; SEQ ID NO: 458; SEQ ID NO: 459; SEQ ID NO: 460; SEQ ID NO: 461; SEQ ID NO: 462; SEQ ID NO: 463; SEQ ID NO: 464; SEQ ID NO: 465; SEQ ID NO: 466; SEQ ID NO: 467; SEQ ID NO: 468; SEQ ID NO: 469; SEQ ID NO: 470; SEQ ID NO: 471; SEQ ID NO: 472; SEQ ID NO: 473; SEQ ID NO: 474; SEQ ID NO: 475; SEQ ID NO: 476; SEQ ID NO: 477; SEQ ID NO: 478; SEQ ID NO: 479; SEQ ID NO: 480; SEQ ID NO: 481; SEQ ID NO: 482; SEQ ID NO: 483; SEQ ID NO: 484; SEQ ID NO: 485; SEQ ID NO: 486; SEQ ID NO: 487; SEQ ID NO: 488; SEQ ID NO: 489; SEQ ID NO: 490; SEQ ID NO: 491; SEQ ID NO: 492; SEQ ID NO: 493; SEQ ID NO: 494; SEQ ID NO: 495; SEQ ID NO: 496; SEQ ID NO: 497; SEQ ID NO: 498; SEQ ID NO: 499; SEQ ID NO: 500; SEQ ID NO: 501; SEQ ID NO: 502; SEQ ID NO: 503; SEQ ID NO: 504; SEQ ID NO: 505; SEQ ID NO: 506; SEQ ID NO: 507; SEQ ID NO: 508; SEQ ID NO: 509; SEQ ID NO: 510; SEQ ID NO: 511; SEQ ID NO: 512; SEQ ID NO: 513; SEQ ID NO: 514; SEQ ID NO: 515; SEQ ID NO: 516; SEQ ID NO: 517; SEQ ID NO: 518; SEQ ID NO: 519; SEQ ID NO: 520; SEQ ID NO: 521; SEQ ID NO: 522; SEQ ID NO: 523; SEQ ID NO: 524; SEQ ID NO: 525; SEQ ID NO: 526; SEQ ID NO: 527; SEQ ID NO: 528; SEQ ID NO: 529; SEQ ID NO: 530; SEQ ID NO: 531; SEQ ID NO: 532; SEQ ID NO: 533; SEQ ID NO: 534; SEQ ID NO: 535; SEQ ID NO: 536; SEQ ID NO: 537; SEQ ID NO: 538; SEQ ID NO: 539; SEQ ID NO: 540; SEQ ID NO: 541; SEQ ID NO: 542; SEQ ID NO: 543; SEQ ID NO: 544; SEQ ID NO: 545; SEQ ID NO: 546; SEQ ID NO: 547; SEQ ID NO: 548; SEQ ID NO: 549; SEQ ID NO: 550; SEQ ID NO: 551; SEQ ID NO: 552; SEQ ID NO: 553; SEQ ID NO: 554; SEQ ID NO: 555; SEQ ID NO: 556; SEQ ID NO: 557; SEQ ID NO: 558; SEQ ID NO: 559; SEQ ID NO: 560; SEQ ID NO: 561; SEQ ID NO: 562; SEQ ID NO: 563; SEQ ID NO: 564; SEQ ID NO: 565; SEQ ID NO: 566; SEQ ID NO: 567; SEQ ID NO: 568; SEQ ID NO: 569; SEQ ID NO: 570; SEQ ID NO: 571; SEQ ID NO: 572; SEQ ID NO: 573; SEQ ID NO: 574; SEQ ID NO: 575; SEQ ID NO: 576; SEQ ID NO: 577; SEQ ID NO: 578; SEQ ID NO: 579; SEQ ID NO: 580; SEQ ID NO: 581; SEQ ID NO: 582; SEQ ID NO: 583; SEQ ID NO: 584; SEQ ID NO: 585; SEQ ID NO: 586; SEQ ID NO: 587; SEQ ID NO: 588; SEQ ID NO: 589; SEQ ID NO: 590; SEQ ID NO: 591; SEQ ID NO: 592; SEQ ID NO: 593; SEQ ID NO: 594; SEQ ID NO: 595; SEQ ID NO: 596; SEQ ID NO: 597; SEQ ID NO: 598; SEQ ID NO: 599; SEQ ID NO: 600; SEQ ID NO: 601; SEQ ID NO: 602; SEQ ID NO: 603; SEQ ID NO: 604; SEQ ID NO: 605; SEQ ID NO: 606; SEQ ID NO: 607; SEQ ID NO: 608; SEQ ID NO: 609; SEQ ID NO: 610; SEQ ID NO: 611; SEQ ID NO: 612; SEQ ID NO: 613; SEQ ID NO: 614; SEQ ID NO: 615; SEQ ID NO: 616; SEQ ID NO: 617; SEQ ID NO: 618; SEQ ID NO: 619; SEQ ID NO: 620; SEQ ID NO: 621; SEQ ID NO: 622; SEQ ID NO: 623; SEQ ID NO-624; SEQ ID NO: 625; SEQ ID NO: 626; SEQ ID NO: 627; SEQ ID NO: 628; SEQ ID NO: 629; SEQ ID NO: 630; SEQ ID NO: 631; SEQ ID NO: 632; SEQ ID NO: 633; SEQ ID NO: 634; SEQ ID NO: 635; SEQ ID NO: 636; SEQ ID NO: 637; SEQ ID NO: 638; SEQ ID NO: 639; SEQ ID NO: 640; SEQ ID NO: 641; SEQ ID NO: 642; SEQ ID NO: 643; SEQ ID NO: 644; SEQ ID NO: 645; SEQ ID NO: 646; SEQ ID NO: 647; SEQ ID NO: 648; SEQ ID NO: 649; SEQ ID NO: 650; SEQ ID NO: 651; SEQ ID NO: 652; SEQ ID NO: 653; SEQ ID NO: 654; SEQ ID NO: 655; SEQ ID NO: 656; SEQ ID NO: 657; SEQ ID NO: 658; SEQ ID NO: 659; SEQ ID NO: 660; SEQ ID NO: 661; SEQ ID NO: 662; SEQ ID NO: 663; SEQ ID NO: 664; SEQ ID NO: 665; SEQ ID NO: 666; SEQ ID NO: 667; SEQ ID NO: 668; SEQ ID NO: 669; SEQ ID NO: 670; SEQ ID NO: 671; SEQ ID NO: 672; SEQ ID NO: 673; SEQ ID NO: 674; SEQ ID NO: 675; SEQ ID NO: 676; SEQ ID NO: 677; SEQ ID NO: 678; SEQ ID NO: 679; SEQ ID NO: 680; SEQ ID NO: 681; SEQ ID NO: 682; SEQ ID NO: 683; SEQ ID NO: 684; SEQ ID NO: 685; SEQ ID NO: 686; SEQ ID NO: 687; SEQ ID NO: 688; SEQ ID NO: 689; SEQ ID NO: 690; SEQ ID NO: 691; SEQ ID NO: 692; SEQ ID NO: 693; SEQ ID NO: 694; SEQ ID NO: 695; SEQ ID NO: 696; SEQ ID NO: 697; SEQ ID NO: 698; SEQ ID NO: 699; SEQ ID NO: 700; SEQ ID NO: 701; SEQ ID NO: 702; SEQ ID NO: 703; SEQ ID NO: 704; SEQ ID NO: 705; SEQ ID NO: 706; SEQ ID NO: 707; SEQ ID NO: 708; SEQ ID NO: 709; SEQ ID NO: 710; SEQ ID NO: 711; SEQ ID NO: 712; SEQ ID NO: 713; SEQ ID NO: 714; SEQ ID NO: 715; SEQ ID NO: 716; SEQ ID NO: 717; SEQ ID NO: 718; SEQ ID NO: 719; SEQ ID NO: 720; SEQ ID NO: 721; SEQ ID NO: 722; SEQ ID NO: 723; SEQ ID NO: 724; SEQ ID NO: 725; SEQ ID NO: 726; SEQ ID NO: 727; SEQ ID NO: 728; SEQ ID NO: 729; SEQ ID NO: 730; SEQ ID NO: 731; SEQ ID NO: 732; SEQ ID NO: 733; SEQ ID NO: 734; SEQ ID NO: 735; SEQ ID NO: 736; SEQ ID NO: 737; SEQ ID NO: 738; SEQ ID NO: 739; SEQ ID NO: 740; SEQ ID NO: 741; SEQ ID NO: 742; SEQ ID NO: 743; SEQ ID NO: 744; SEQ ID NO: 745; SEQ ID NO: 746; SEQ ID NO: 747; SEQ ID NO: 748; SEQ ID NO: 749; SEQ ID NO: 750; SEQ ID NO: 751; SEQ ID NO: 752; SEQ ID NO: 753; SEQ ID NO: 754; SEQ ID NO: 755; SEQ ID NO: 756; SEQ ID NO: 757; SEQ ID NO: 758; SEQ ID NO: 759; SEQ ID NO: 760; SEQ ID NO: 761; SEQ ID NO: 762; SEQ ID NO: 763; SEQ ID NO: 764; SEQ ID NO: 765; SEQ ID NO: 766; SEQ ID NO: 767; SEQ ID NO: 768; SEQ ID NO: 769; SEQ ID NO: 770; SEQ ID NO: 771; SEQ ID NO: 772; SEQ ID NO: 773; SEQ ID NO: 774; SEQ ID NO: 775; SEQ ID NO: 776; SEQ ID NO: 777; SEQ ID NO: 778; SEQ ID NO: 779; SEQ ID NO: 780; SEQ ID NO: 781; SEQ ID NO: 782; SEQ ID NO: 783; SEQ ID NO: 784; SEQ ID NO: 785; SEQ ID NO: 786; SEQ ID NO: 787; SEQ ID NO: 788; SEQ ID NO: 789; SEQ ID NO: 790; SEQ ID NO: 791; SEQ ID NO: 792; SEQ ID NO: 793; SEQ ID NO: 794; SEQ ID NO: 795; SEQ ID NO: 796; SEQ ID NO: 797; SEQ ID NO: 798; SEQ ID NO: 799; SEQ ID NO: 800; SEQ ID NO: 801; SEQ ID NO: 802; SEQ ID NO: 803; SEQ ID NO: 804; SEQ ID NO: 805; SEQ ID NO: 806; SEQ ID NO: 807; SEQ ID NO: 808; SEQ ID NO: 809; SEQ ID NO: 810; SEQ ID NO: 811; SEQ ID NO: 812; SEQ ID NO: 813; SEQ ID NO: 814; SEQ ID NO: 815; SEQ ID NO: 816; SEQ ID NO: 817; SEQ ID NO: 818; SEQ ID NO: 819; SEQ ID NO: 820; SEQ ID NO: 821; SEQ ID NO: 822; SEQ ID NO: 823; SEQ ID NO: 824; SEQ ID NO: 825; SEQ ID NO: 826; SEQ ID NO: 827; SEQ ID NO: 828; SEQ ID NO: 829; SEQ ID NO: 830; SEQ ID NO: 831; SEQ ID NO: 832; SEQ ID NO: 833; SEQ ID NO: 834; SEQ ID NO: 835; SEQ ID NO: 836; SEQ ID NO: 837; SEQ ID NO: 838; SEQ ID NO: 839; SEQ ID NO: 840; SEQ ID NO: 841; SEQ ID NO: 842; SEQ ID NO: 843; SEQ ID NO: 844; SEQ ID NO: 845; SEQ ID NO: 846; SEQ ID NO: 847; SEQ ID NO: 848; SEQ ID NO: 849; SEQ ID NO: 850; SEQ ID NO: 851; SEQ ID NO: 852; SEQ ID NO: 853; SEQ ID NO: 854; SEQ ID NO: 855; SEQ ID NO: 856; SEQ ID NO: 857; SEQ ID NO: 858; SEQ ID NO: 859; SEQ ID NO: 860; SEQ ID NO: 861; SEQ ID NO: 862; SEQ ID NO: 863; SEQ ID NO: 864; SEQ ID NO: 865; SEQ ID NO: 866; SEQ ID NO: 867; SEQ ID NO: 868; SEQ ID NO: 869; SEQ ID NO: 870; SEQ ID NO: 871; SEQ ID NO: 872; SEQ ID NO: 873; SEQ ID NO: 874; SEQ ID NO: 875; SEQ ID NO: 876; SEQ ID NO: 877; SEQ ID NO: 878; SEQ ID NO: 879; SEQ ID NO: 880; SEQ ID NO: 881; SEQ ID NO: 882; SEQ ID NO: 883; SEQ ID NO: 884; SEQ ID NO: 885; SEQ ID NO: 886; SEQ ID NO: 887; SEQ ID NO: 888; SEQ ID NO: 889; SEQ ID NO: 890; SEQ ID NO: 891; SEQ ID NO: 892; SEQ ID NO: 893; SEQ ID NO: 894; SEQ ID NO: 895; SEQ ID NO: 896; SEQ ID NO: 897; SEQ ID NO: 898; SEQ ID NO: 899; SEQ ID NO: 900; SEQ ID NO: 901; SEQ ID NO: 902; SEQ ID NO: 903; SEQ ID NO: 904; SEQ ID NO: 905; SEQ ID NO: 906; SEQ ID NO: 907; SEQ ID NO: 908; SEQ ID NO: 909; SEQ ID NO: 910; SEQ ID NO: 911; SEQ ID NO: 912; SEQ ID NO: 913; SEQ ID NO: 914; SEQ ID NO: 915; SEQ ID NO: 916; SEQ ID NO: 917; SEQ ID NO: 918; SEQ ID NO: 919; SEQ ID NO: 920; SEQ ID NO: 921; SEQ ID NO: 922; SEQ ID NO: 923; SEQ ID NO: 924; SEQ ID NO: 925; SEQ ID NO: 926; SEQ ID NO: 927; SEQ ID NO: 928; SEQ ID NO: 929; SEQ ID NO: 930; SEQ ID NO: 931; SEQ ID NO: 932; SEQ ID NO: 933; SEQ ID NO: 934; SEQ ID NO: 935; SEQ ID NO: 936; SEQ ID NO: 937; SEQ ID NO: 938; SEQ ID NO: 939; SEQ ID NO: 940; SEQ ID NO: 941; SEQ ID NO: 942; SEQ ID NO: 943; SEQ ID NO: 944; SEQ ID NO: 945; SEQ ID NO: 946; SEQ ID NO: 947; SEQ ID NO: 948; SEQ ID NO: 949; SEQ ID NO: 950; SEQ ID NO: 951; SEQ ID NO: 952; SEQ ID NO: 953; SEQ ID NO: 954; SEQ ID NO: 955; SEQ ID NO: 956; SEQ ID NO: 957; SEQ ID NO: 958; SEQ ID NO: 959; SEQ ID NO: 960; SEQ ID NO: 961; SEQ ID NO: 962; SEQ ID NO: 963; SEQ ID NO: 964; SEQ ID NO: 965; SEQ ID NO: 966; SEQ ID NO: 967; SEQ ID NO: 968; SEQ ID NO: 969; SEQ ID NO: 970; SEQ ID NO: 971; SEQ ID NO: 972; SEQ ID NO: 973; SEQ ID NO: 974; SEQ ID NO: 975; SEQ ID NO: 976; SEQ ID NO: 977; SEQ ID NO: 978; SEQ ID NO: 979; SEQ ID NO: 980; SEQ ID NO: 981; SEQ ID NO: 982; SEQ ID NO: 983; SEQ ID NO: 984; SEQ ID NO: 985; SEQ ID NO: 986; SEQ ID NO: 987; SEQ ID NO: 988; SEQ ID NO: 989; SEQ ID NO: 990; SEQ ID NO: 991; SEQ ID NO: 992; SEQ ID NO: 993; SEQ ID NO: 994; SEQ ID NO: 995; SEQ ID NO: 996; SEQ ID NO: 997; SEQ ID NO: 998; SEQ ID NO: 999; SEQ ID NO: 1000; SEQ ID NO: 1001; SEQ ID NO: 1002; SEQ ID NO: 1003; SEQ ID NO: 1004; SEQ ID NO: 1005; SEQ ID NO: 1006; SEQ ID NO: 1007; SEQ ID NO: 1008; SEQ ID NO:1009; SEQ ID NO: 1010; SEQ ID NO:1011; SEQ ID NO: 1012; SEQ ID NO: 1013; SEQ ID NO: 1014; SEQ ID NO: 1015; SEQ ID NO: 1016; SEQ ID NO: 1017; SEQ ID NO: 1018; SEQ ID NO: 1019; SEQ ID NO: 1020; SEQ ID NO: 1021; SEQ ID NO: 1.022; SEQ ID NO: 1023; SEQ ID NO: 1024; SEQ ID NO: 1025; SEQ ID NO: 1026; SEQ ID NO: 1027; SEQ ID NO: 1028; SEQ ID NO: 1029; SEQ ID NO: 1030; SEQ ID NO: 1031; SEQ ID NO: 1032; SEQ ID NO: 1033; SEQ ID NO: 1034; SEQ ID NO: 1035; SEQ ID NO: 1036; SEQ ID NO: 1037; SEQ ID NO: 1038; SEQ ID NO: 1039; SEQ ID NO: 1040; SEQ ID NO: 1041; SEQ ID NO: 1042; SEQ ID NO: 1043; SEQ ID NO: 1044; SEQ ID NO: 1045; SEQ ID NO: 1046; SEQ ID NO: 1047; SEQ ID NO: 1048; SEQ ID NO: 1049; SEQ ID NO: 1050; SEQ ID NO: 1051; SEQ ID NO: 1052; SEQ ID NO: 1053; SEQ ID NO: 1054; SEQ ID NO: 1055; SEQ ID NO: 1056; SEQ ID NO: 1057; SEQ ID NO: 1058; SEQ ID NO: 1059; SEQ ID NO: 1060; SEQ ID NO: 1061; SEQ ID NO: 1062; SEQ ID NO: 1063; SEQ ID NO: 1064; SEQ ID NO: 1065; SEQ ID NO: 1066; SEQ ID NO: 1067; SEQ ID NO: 1068; SEQ ID NO: 1069; SEQ ID NO: 1070; SEQ ID NO: 1071; SEQ ID NO: 1072; SEQ ID NO: 1073; SEQ ID NO: 1074; SEQ ID NO: 1075; SEQ ID NO: 1076; SEQ ID NO: 1077; SEQ ID NO: 1078; SEQ ID NO: 1079; SEQ ID NO: 1080; SEQ ID NO: 1081; SEQ ID NO: 1082; SEQ ID NO: 1083; SEQ ID NO: 1084; SEQ ID NO: 1085; SEQ ID NO: 1086; SEQ ID NO: 1087; SEQ ID NO: 1088; SEQ ID NO: 1089; SEQ ID NO: 1090; SEQ ID NO: 1091; SEQ ID NO: 1092; SEQ ID NO: 1093; SEQ ID NO: 1094; SEQ ID NO: 1095; SEQ ID NO: 1096; SEQ ID NO: 1097; SEQ ID NO: 1098; SEQ ID NO: 1099; SEQ ID NO: 1100; SEQ ID NO: 1101; SEQ ID NO: 1102; SEQ ID NO: 1103; SEQ ID NO: 1104; SEQ ID NO: 1105; SEQ ID NO: 1106; SEQ ID NO: 1107; SEQ ID NO: 1108; SEQ ID NO:1109; SEQ ID NO: 1110; SEQ ID NO: 1111; SEQ ID NO: 1112; SEQ ID NO: 1113; SEQ ID NO: 1114; SEQ ID NO: 1115; SEQ ID NO: 1116; SEQ ID NO: 1117; SEQ ID NO: 1118; SEQ ID NO:1119; SEQ ID NO: 1120; SEQ ID NO: 1121; SEQ ID NO: 1122; SEQ ID NO: 1123; SEQ ID NO: 1124; SEQ ID NO: 1125; SEQ ID NO: 1126; SEQ ID NO: 1127; SEQ ID NO: 1128; SEQ ID NO: 1129; SEQ ID NO: 1130; SEQ ID NO: 1131; SEQ ID NO: 1132; SEQ ID NO: 1133; SEQ ID NO: 1134; SEQ ID NO: 1135; SEQ ID NO: 1136; SEQ ID NO: 1137; SEQ ID NO: 1138; SEQ ID NO: 1139; SEQ ID NO: 1140; SEQ ID NO: 1141; SEQ ID NO: 1142; SEQ ID NO: 1143; SEQ ID NO: 1144; SEQ ID NO: 1145; SEQ ID NO: 1146; SEQ ID NO: 1147; SEQ ID NO: 1148; SEQ ID NO: 1149; SEQ ID NO: 1150; SEQ ID NO: 1151; SEQ ID NO: 1152; SEQ ID NO: 1153; SEQ ID NO: 1154; SEQ ID NO: 1155; SEQ ID NO: 1156; SEQ ID NO: 1157; SEQ ID NO: 1158; SEQ ID NO: 1159; SEQ ID NO: 1160; SEQ ID NO: 1161; SEQ ID NO: 1162; SEQ ID NO: 1163; SEQ ID NO: 1164; SEQ ID NO: 1165; SEQ ID NO: 1166; SEQ ID NO: 1167; SEQ ID NO: 1168; SEQ ID NO: 1169; SEQ ID NO: 1170; SEQ ID NO: 1171; SEQ ID NO: 1172; SEQ ID NO: 1173; SEQ ID NO:1174; SEQ ID NO: 1175; SEQ ID NO:1176; SEQ ID NO: 1177; SEQ ID NO: 1178; SEQ ID NO: 1179; SEQ ID NO: 1180; SEQ ID NO: 1181; SEQ ID NO: 1182; SEQ ID NO: 1183; SEQ ID NO: 1184; SEQ ID NO: 1185; SEQ ID NO: 1186; SEQ ID NO: 1187; SEQ ID NO: 1188; SEQ ID NO: 1189; SEQ ID NO: 1190; SEQ ID NO: 1191; SEQ ID NO: 1192; SEQ ID NO: 1193; SEQ ID NO: 1194; SEQ ID NO: 1195; SEQ ID NO: 1196; SEQ ID NO: 1197; SEQ ID NO: 1198; SEQ ID NO: 1199; SEQ ID NO: 1200; SEQ ID NO: 1201; SEQ ID NO: 1202; SEQ ID NO: 1203; SEQ ID NO: 1204; SEQ ID NO: 1205; SEQ ID NO: 1206; SEQ ID NO: 1207; SEQ ID NO: 1208; SEQ ID NO: 1209; SEQ ID NO: 1210; SEQ ID NO: 1211; SEQ ID NO: 1212; SEQ ID NO: 1213; SEQ ID NO: 1214; SEQ ID NO: 1215; SEQ ID NO: 1216; SEQ ID NO: 1217; SEQ ID NO: 1218; SEQ ID NO: 1219; SEQ ID NO: 1220; SEQ ID NO: 1221; SEQ ID NO: 1222; SEQ ID NO: 1223; SEQ ID NO: 1224; SEQ ID NO: 1225; SEQ ID NO: 1226; SEQ ID NO: 1227; SEQ ID NO: 1228; SEQ ID NO: 1229; SEQ ID NO: 1230; SEQ ID NO: 1231; SEQ ID NO: 1232; SEQ ID NO: 1233; SEQ ID NO: 1234; SEQ ID NO: 1235; SEQ ID NO: 1236; SEQ ID NO: 1237; SEQ ID NO: 1238; SEQ ID NO: 1239; SEQ ID NO: 1240; SEQ ID NO: 1241; SEQ ID NO: 1242; SEQ ID NO: 1243; SEQ ID NO: 1244; SEQ ID NO: 1245; SEQ ID NO: 1246; SEQ ID NO: 1247; SEQ ID NO: 1248; SEQ ID NO: 1249; SEQ ID NO: 1250; SEQ ID NO: 1251; SEQ ID NO: 1252; SEQ ID NO: 1253; SEQ ID NO: 1254; SEQ ID NO: 1255; SEQ ID NO: 1256; SEQ ID NO: 1257; SEQ ID NO: 1258; SEQ ID NO: 1259; SEQ ID NO: 1260; SEQ ID NO: 1261; SEQ ID NO: 1262; SEQ ID NO: 1263; SEQ ID NO: 1264; SEQ ID NO: 1265; SEQ ID NO: 1266; SEQ ID NO: 1267; SEQ ID NO: 1268; SEQ ID NO: 1269; SEQ ID NO: 1270; SEQ ID NO: 1271; SEQ ID NO: 1272; SEQ ID NO: 1273; SEQ ID NO: 1274; SEQ ID NO: 1275; SEQ ID NO: 1276; SEQ ID NO: 1277; SEQ ID NO: 1278; SEQ ID NO: 1279; SEQ ID NO: 1280; SEQ ID NO: 1281; SEQ ID NO: 1282; SEQ ID NO: 1283; SEQ ID NO: 1284; SEQ ID NO: 1285; SEQ ID NO: 1286; SEQ ID NO: 1287; SEQ ID NO: 1288; SEQ ID NO: 1289; SEQ ID NO: 1290; SEQ ID NO: 1291; SEQ ID NO: 1292; SEQ ID NO: 1293; SEQ ID NO: 1294; SEQ ID NO: 1295; SEQ ID NO: 1296; SEQ ID NO: 1297; SEQ ID NO: 1298; SEQ ID NO: 1299; SEQ ID NO: 1300; SEQ ID NO: 1301; SEQ ID NO: 1302; SEQ ID NO: 1303; SEQ ID NO: 1304; SEQ ID NO: 1305; SEQ ID NO: 1306; SEQ ID NO: 1307; SEQ ID NO: 1308; SEQ ID NO: 1309; SEQ ID NO: 1310; SEQ ID NO: 1311I; SEQ ID NO: 1312; SEQ ID NO: 1313; SEQ ID NO: 1314; SEQ ID NO: 1315; SEQ ID NO: 1316; SEQ ID NO: 1317; SEQ ID NO: 1318; SEQ ID NO: 1319; SEQ ID NO: 1320; SEQ ID NO: 1321; SEQ ID NO: 1322; SEQ ID NO: 1323; SEQ ID NO: 1324; SEQ ID NO: 1325; SEQ ID NO: 1326; SEQ ID NO: 1327; SEQ ID NO: 1328; SEQ ID NO: 1329; SEQ ID NO: 1330; SEQ ID NO: 1331; SEQ ID NO: 1332; SEQ ID NO: 1333; SEQ ID NO: 1334; SEQ ID NO: 1335; SEQ ID NO: 1336; SEQ ID NO: 1337; SEQ ID NO: 1338; SEQ ID NO: 1339; SEQ ID NO: 1340; SEQ ID NO: 1341; SEQ ID NO: 1342; SEQ ID NO: 1343; SEQ ID NO: 1344; SEQ ID NO: 1345; SEQ ID NO: 1346; SEQ ID NO: 1347; SEQ ID NO: 1348; SEQ ID NO: 1349; SEQ ID NO: 1350; SEQ ID NO: 1351; SEQ ID NO: 1352; SEQ ID NO: 1353; SEQ ID NO: 1354; SEQ ID NO: 1355; SEQ ID NO: 1356; SEQ ID NO: 1357; SEQ ID NO: 1358; SEQ ID NO: 1359; SEQ ID NO: 1360; SEQ ID NO: 1361; SEQ ID NO: 1362; SEQ ID NO: 1363; SEQ ID NO: 1364; SEQ ID NO: 1365; SEQ ID NO: 1366; SEQ ID NO: 1367; SEQ ID NO: 1368; SEQ ID NO: 1369; SEQ ID NO: 1370; SEQ ID NO: 1371; SEQ ID NO: 1372; SEQ ID NO: 1373; SEQ ID NO: 1374; SEQ ID NO: 1375; SEQ ID NO: 1376; SEQ ID NO: 1377; SEQ ID NO: 1378; SEQ ID NO: 1379; SEQ ID NO: 1380; SEQ ID NO: 1381; SEQ ID NO: 1382; SEQ ID NO: 1383; SEQ ID NO: 1384; SEQ ID NO: 1385; SEQ ID NO: 1386; SEQ ID NO: 1387; SEQ ID NO: 1388; SEQ ID NO: 1389; SEQ ID NO: 1390; SEQ ID NO: 1391; SEQ ID NO: 1392; SEQ ID NO: 1393; SEQ ID NO: 1394; SEQ ID NO: 1395; SEQ ID NO: 1396; SEQ ID NO: 1397; SEQ ID NO: 1398; SEQ ID NO: 1399; SEQ ID NO: 1400; SEQ ID NO: 1401; SEQ ID NO: 1402; SEQ ID NO: 1403; SEQ ID NO: 1404; SEQ ID NO: 1405; SEQ ID NO: 1406; SEQ ID NO: 1407; SEQ ID NO: 1408; SEQ ID NO: 1409; SEQ ID NO: 1410; SEQ ID NO: 1411; SEQ ID NO: 1412; SEQ ID NO: 1413; SEQ ID NO: 1414; SEQ ID NO: 1415; SEQ ID NO: 1416; SEQ ID NO: 1417; SEQ ID NO: 1418; SEQ ID NO: 1419; SEQ ID NO: 1420; SEQ ID NO: 1421; SEQ ID NO: 1422; SEQ ID NO: 1423; SEQ ID NO: 1424; SEQ ID NO: 1425; SEQ ID NO: 1426; SEQ ID NO: 1427; SEQ ID NO: 1428; SEQ ID NO: 1429; SEQ ID NO: 1430; SEQ ID NO: 1431; SEQ ID NO: 1432; SEQ ID NO: 1433; SEQ ID NO: 1434; SEQ ID NO: 1435; SEQ ID NO: 1436; SEQ ID NO: 1437; SEQ ID NO: 1438; SEQ ID NO: 1439; SEQ ID NO: 1440; SEQ ID NO: 1441; SEQ ID NO: 1442; SEQ ID NO: 1443; SEQ ID NO: 1444; SEQ ID NO: 1445; SEQ ID NO: 1446; SEQ ID NO: 1447; SEQ ID NO: 1448; SEQ ID NO: 1449; SEQ ID NO: 1450; SEQ ID NO: 1451; SEQ ID NO: 1452; SEQ ID NO: 1453; SEQ ID NO: 1454; SEQ ID NO: 1455; SEQ ID NO: 1456; SEQ ID NO: 1457; SEQ ID NO: 1458; SEQ ID NO: 1459; SEQ ID NO: 1460; SEQ ID NO: 1461; SEQ ID NO: 1462; SEQ ID NO: 1463; SEQ ID NO: 1464; SEQ ID NO: 1465; SEQ ID NO: 1466; SEQ ID NO: 1467; SEQ ID NO: 1468; SEQ ID NO: 1469; SEQ ID NO: 1470; SEQ ID NO: 1471; SEQ ID NO: 1472; SEQ ID NO: 1473; SEQ ID NO: 1474; SEQ ID NO: 1475; SEQ ID NO: 1476; SEQ ID NO: 1477; SEQ ID NO: 1478; SEQ ID NO: 1479; SEQ ID NO: 1480; SEQ ID NO: 1481; SEQ ID NO: 1482; SEQ ID NO: 1483; SEQ ID NO: 1484; SEQ ID NO: 1485; SEQ ID NO: 1486; SEQ ID NO: 1487; SEQ ID NO: 1488; SEQ ID NO: 1489; SEQ ID NO: 1490; SEQ ID NO: 1491; SEQ ID NO: 1492; SEQ ID NO: 1493; SEQ ID NO: 1494; SEQ ID NO: 1495; SEQ ID NO: 1496; SEQ ID NO: 1497; SEQ ID NO: 1498; SEQ ID NO: 1499; SEQ ID NO: 1500; SEQ ID NO: 1501; SEQ ID NO: 1502; SEQ ID NO: 1503; SEQ ID NO: 1504; SEQ ID NO: 1505; SEQ ID NO: 1506; SEQ ID NO: 1507; SEQ ID NO: 1508; SEQ ID NO: 1509; SEQ ID NO: 1510; SEQ ID NO: 1511; SEQ ID NO: 1512; SEQ ID NO: 1513; SEQ ID NO: 1514; SEQ ID NO: 1515; SEQ ID NO: 1516; SEQ ID NO: 1517; SEQ ID NO: 1518; SEQ ID NO: 1519; SEQ ID NO: 1520; SEQ ID NO: 1521; SEQ ID NO: 1522; SEQ ID NO: 1523; SEQ ID NO: 1524; SEQ ID NO: 1525; SEQ ID NO: 1526; SEQ ID NO: 1527; SEQ ID NO: 1528; SEQ ID NO: 1529; SEQ ID NO: 1530; SEQ ID NO: 1531; SEQ ID NO: 1532; SEQ ID NO: 1533; SEQ ID NO: 1534; SEQ ID NO: 1535; SEQ ID NO: 1536; SEQ ID NO: 1537; SEQ ID NO: 1538; SEQ ID NO: 1539; SEQ ID NO: 1540; SEQ ID NO: 1541; SEQ ID NO: 1542; SEQ ID NO: 1543; SEQ ID NO: 1544; SEQ ID NO: 1545; SEQ ID NO: 1546; SEQ ID NO: 1547; SEQ ID NO: 1548; SEQ ID NO: 1549; SEQ ID NO: 1550; SEQ ID NO: 1551; SEQ ID NO: 1552; SEQ ID NO: 1553; SEQ ID NO: 1554; SEQ ID NO: 1555; SEQ ID NO: 1556; SEQ ID NO: 1557; SEQ ID NO: 1558; SEQ ID NO: 1559; SEQ ID NO: 1560; SEQ ID NO: 1561; SEQ ID NO: 1562; SEQ ID NO: 1563; SEQ ID NO: 1564; SEQ ID NO: 1565; SEQ ID NO: 1566; SEQ ID NO: 1567; SEQ ID NO: 1568; SEQ ID NO: 1569; SEQ ID NO: 1570; SEQ ID NO: 1571; SEQ ID NO: 1572; SEQ ID NO: 1573; SEQ ID NO: 1574; SEQ ID NO: 1575; SEQ ID NO: 9525; SEQ ID NO: 9526; SEQ ID NO: 9527; SEQ ID NO: 9528; SEQ ID NO: 9529; SEQ ID NO: 9530; SEQ ID NO: 9531; SEQ ID NO: 9532; SEQ ID NO: 9533; SEQ ID NO: 9534; SEQ ID NO: 9535; SEQ ID NO: 9536; SEQ ID NO: 9537; SEQ ID NO: 9538; SEQ ID NO: 9539; SEQ ID NO: 9540; SEQ ID NO: 9541; SEQ ID NO: 9542; SEQ ID NO: 9543; SEQ ID NO: 9544; SEQ ID NO: 9545; SEQ ID NO: 9546; SEQ ID NO: 9547; SEQ ID NO: 9548; SEQ ID NO: 9549; SEQ ID NO: 9550; SEQ ID NO: 9551; SEQ ID NO: 9552; SEQ ID NO: 9553; SEQ ID NO: 9554; SEQ ID NO: 9555; SEQ ID NO: 9556; SEQ ID NO: 9557; SEQ ID NO: 9558; SEQ ID NO: 9559; SEQ ID NO: 9560; SEQ ID NO: 9561; SEQ ID NO: 9562; SEQ ID NO: 9563; SEQ ID NO: 9564; SEQ ID NO: 9565; SEQ ID NO: 9566; SEQ ID NO: 9567; SEQ ID NO: 9568; SEQ ID NO: 9569; SEQ ID NO: 9570; SEQ ID NO: 9571; SEQ ID NO: 9572; SEQ ID NO: 9573; SEQ ID NO: 9574; SEQ ID NO: 9575; SEQ ID NO: 9576; SEQ ID NO: 9577; SEQ ID NO: 9578; SEQ ID NO: 9579; SEQ ID NO: 9580; SEQ ID NO: 9581; SEQ ID NO: 9582; SEQ ID NO: 9583; SEQ ID NO: 9584; SEQ ID NO: 9585; SEQ ID NO: 9586; SEQ ID NO: 9587; SEQ ID NO: 9588; SEQ ID NO: 9589; SEQ ID NO: 9590; SEQ ID NO: 9591 and SEQ ID NO: 9592.  
     [0117] In one embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  flagella-associated polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1; SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 5; SEQ ID NO: 6; SEQ ID NO: 7; SEQ ID NO: 8; SEQ ID NO: 9; SEQ ID NO: 10; SEQ ID NO: 11; SEQ ID NO: 12; SEQ ID NO: 13; SEQ ID NO: 14; SEQ ID NO: 15; SEQ ID NO: 16; SEQ ID NO: 17; SEQ ID NO: 18; SEQ ID NO: 19; SEQ ID NO: 20; SEQ ID NO: 21; SEQ ID NO: 22; SEQ ID NO: 23; SEQ ID NO: 24; SEQ ID NO: 25; SEQ ID NO: 26; SEQ ID NO: 27; SEQ ID NO: 28; SEQ ID NO: 29; SEQ ID NO: 30; SEQ ID NO: 31; SEQ ID NO: 32; SEQ ID NO: 33; SEQ ID NO: 34; SEQ ID NO: 35; SEQ ID NO: 36; SEQ ID NO: 37; SEQ ID NO: 38; SEQ ID NO: 39; SEQ ID NO: 40; SEQ ID NO: 41; SEQ ID NO: 42; SEQ ID NO: 43; SEQ ID NO: 44; SEQ ID NO: 45; SEQ ID NO: 46; SEQ ID NO: 47; SEQ ID NO: 48; SEQ ID NO: 49; SEQ ID NO: 50; SEQ ID NO: 51; SEQ ID NO: 52; SEQ ID NO: 53; SEQ ID NO: 54; SEQ ID NO: 55; SEQ ID NO: 56; SEQ ID NO: 57; SEQ ID NO: 58; SEQ ID NO: 59; SEQ ID NO: 60; SEQ ID NO: 61; SEQ ID NO: 62; SEQ ID NO: 63; SEQ ID NO: 64; SEQ ID NO: 65; SEQ ID NO: 66; SEQ ID NO: 67; SEQ ID NO: 68; SEQ ID NO: 69; SEQ ID NO: 70; SEQ ID NO: 71; SEQ ID NO: 72; SEQ ID NO: 73; SEQ ID NO: 74; SEQ ID NO: 75; SEQ ID NO: 76; SEQ ID NO: 77; SEQ ID NO: 78; SEQ ID NO: 79; SEQ ID NO: 80; SEQ ID NO: 81; SEQ ID NO: 82; SEQ ID NO: 83; SEQ ID NO: 84; SEQ ID NO: 85; SEQ ID NO: 86; SEQ ID NO: 87; SEQ ID NO: 88; SEQ ID NO: 89; SEQ ID NO: 90; SEQ ID NO: 91; SEQ ID NO: 92; SEQ ID NO: 93; SEQ ID NO: 94; SEQ ID NO: 95; SEQ ID NO: 96; SEQ ID NO:97; SEQ ID NO: 98; SEQ ID NO: 99; SEQ ID NO: 100; SEQ ID NO: 101; SEQ ID NO: 102; SEQ ID NO: 103; SEQ ID NO: 9525; SEQ ID NO: 9526 and SEQ ID NO: 9527.  
     [0118] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  outer membrane polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 104; SEQ ID NO: 105; SEQ ID NO: 106; SEQ ID NO: 107; SEQ ID NO: 108; SEQ ID NO: 109; SEQ ID NO: 110; SEQ ID NO:111; SEQ ID NO: 112; SEQ ID NO: 113; SEQ ID NO: 114; SEQ ID NO: 115; SEQ ID NO: 116; SEQ ID NO: 117; SEQ ID NO: 118; SEQ ID NO: 119; SEQ ID NO: 120; SEQ ID NO: 121; SEQ ID NO: 122; SEQ ID NO: 123; SEQ ID NO: 124; SEQ ID NO: 125; SEQ ID NO: 126; SEQ ID NO: 127; SEQ ID NO: 128; SEQ ID NO: 129; SEQ ID NO: 130; SEQ ID NO: 131; SEQ ID NO: 132; SEQ ID NO: 133; SEQ ID NO: 134; SEQ ID NO: 135; SEQ ID NO: 136; SEQ ID NO: 137; SEQ ID NO: 138; SEQ ID NO: 139; SEQ ID NO: 140; SEQ ID NO: 141; SEQ ID NO: 142; SEQ ID NO: 143; SEQ ID NO: 144; SEQ ID NO: 145: SEQ ID NO: 146; SEQ ID NO: 147; SEQ ID NO: 148; SEQ ID NO: 149; SEQ ID NO: 150; SEQ ID NO: 151; SEQ ID NO: 152; SEQ ID NO: 153; SEQ ID NO: 154; SEQ ID NO: 155; SEQ ID NO: 156; SEQ ID NO: 157; SEQ ID NO: 158; SEQ ID NO: 159; SEQ ID NO: 160; SEQ ID NO: 161; SEQ ID NO: 162; SEQ ID NO: 163; SEQ ID NO: 164; SEQ ID NO: 165; SEQ ID NO: 166; SEQ ID NO: 167; SEQ ID NO: 168; SEQ ID NO: 169; SEQ ID NO: 170; SEQ ID NO: 171; SEQ ID NO: 172; SEQ ID NO: 173; SEQ ID NO: 174; SEQ ID NO: 175; SEQ ID NO: 176; SEQ ID NO: 177; SEQ ID NO: 178; SEQ ID NO: 179; SEQ ID NO: 180; SEQ ID NO: 181; SEQ ID NO: 182; SEQ ID NO: 183; SEQ ID NO: 184; SEQ ID NO: 185; SEQ ID NO: 186; SEQ ID NO: 187; SEQ ID NO: 188; SEQ ID NO: 189; SEQ ID NO: 190; SEQ ID NO: 191; SEQ ID NO: 192; SEQ ID NO: 193; SEQ ID NO: 194; SEQ ID NO: 195; SEQ ID NO: 196; SEQ ID NO: 197; SEQ ID NO: 198; SEQ ID NO: 199; SEQ ID NO: 200; SEQ ID NO: 201; SEQ ID NO: 202; SEQ ID NO: 203; SEQ ID NO: 204; SEQ ID NO: 205; SEQ ID NO: 206; SEQ ID NO: 207; SEQ ID NO: 208; SEQ ID NO: 209; SEQ ID NO: 210; SEQ ID NO: 211; SEQ ID NO: 212; SEQ ID NO: 213; SEQ ID NO: 214; SEQ ID NO: 215; SEQ ID NO: 216; SEQ ID NO: 217; SEQ ID NO: 218; SEQ ID NO: 219; SEQ ID NO: 220; SEQ ID NO: 221; SEQ ID NO: 222; SEQ ID NO: 223; SEQ ID NO: 224; SEQ ID NO: 225; SEQ ID NO: 226; SEQ ID NO: 227; SEQ ID NO: 228; SEQ ID NO: 229; SEQ ID NO: 230; SEQ ID NO: 231; SEQ ID NO: 232; SEQ ID NO: 233; SEQ ID NO: 234; SEQ ID NO: 235; SEQ ID NO: 236; SEQ ID NO: 237; SEQ ID NO: 238; SEQ ID NO: 239; SEQ ID NO: 240; SEQ ID NO: 241; SEQ ID NO: 242; SEQ ID NO: 243; SEQ ID NO: 244; SEQ ID NO: 245; SEQ ID NO: 246; SEQ ID NO: 247; SEQ ID NO: 248; SEQ ID NO: 249; SEQ ID NO: 250; SEQ ID NO:251; SEQ ID NO: 252; SEQ ID NO: 253; SEQ ID NO: 254; SEQ ID NO: 255; SEQ ID NO: 256; SEQ ID NO: 257; SEQ ID NO: 258; SEQ ID NO: 259; SEQ ID NO: 260; SEQ ID NO: 261; SEQ ID NO: 262; SEQ ID NO: 263; SEQ ID NO: 264; SEQ ID NO: 265; SEQ ID NO: 266; SEQ ID NO: 267; SEQ ID NO: 268; SEQ ID NO: 269; SEQ ID NO: 270; SEQ ID NO: 271; SEQ ID NO: 272; SEQ ID NO: 273; SEQ ID NO: 274; SEQ ID NO: 275; SEQ ID NO: 276; SEQ ID NO: 277; SEQ ID NO: 278; SEQ ID NO: 279; SEQ ID NO: 280; SEQ ID NO: 281; SEQ ID NO: 282; SEQ ID NO: 283; SEQ ID NO: 284; SEQ ID NO: 285; SEQ ID NO: 286; SEQ ID NO: 287; SEQ ID NO: 288; SEQ ID NO: 289; SEQ ID NO: 290; SEQ ID NO: 291; SEQ ID NO: 292; SEQ ID NO: 293; SEQ ID NO: 294; SEQ ID NO: 295; SEQ ID NO: 296; SEQ ID NO: 297; SEQ ID NO: 298; SEQ ID NO: 299; SEQ ID NO: 300; SEQ ID NO: 301; SEQ ID NO: 302; SEQ ID NO: 303; SEQ ID NO: 304; SEQ ID NO: 305; SEQ ID NO: 306; SEQ ID NO: 307; SEQ ID NO: 308; SEQ ID NO: 309; SEQ ID NO: 310; SEQ ID NO: 311; SEQ ID NO:312; SEQ ID NO:313; SEQ ID NO: 314; SEQ ID NO: 315; SEQ ID NO: 316; SEQ ID NO: 317; SEQ ID NO: 318; SEQ ID NO: 319; SEQ ID NO: 320; SEQ ID NO: 321; SEQ ID NO: 322; SEQ ID NO: 323; SEQ ID NO: 324; SEQ ID NO: 325; SEQ ID NO: 326; SEQ ID NO: 327; SEQ ID NO: 328; SEQ ID NO: 329; SEQ ID NO: 330; SEQ ID NO: 331; SEQ ID NO: 332; SEQ ID NO: 333; SEQ ID NO: 334; SEQ ID NO: 335; SEQ ID NO: 336; SEQ ID NO: 337; SEQ ID NO: 338; SEQ ID NO: 339; SEQ ID NO: 340; SEQ ID NO: 341; SEQ ID NO: 342; SEQ ID NO: 343; SEQ ID NO: 344; SEQ ID NO: 345; SEQ ID NO: 346; SEQ ID NO: 347; SEQ ID NO: 348; SEQ ID NO: 349; SEQ ID NO: 350; SEQ ID NO: 351; SEQ ID NO: 352; SEQ ID NO: 353; SEQ ID NO: 354; SEQ ID NO: 355; SEQ ID NO: 356; SEQ ID NO: 357; SEQ ID NO: 358; SEQ ID NO: 359; SEQ ID NO: 360; SEQ ID NO: 361; SEQ ID NO: 362; SEQ ID NO: 363; SEQ ID NO: 364; SEQ ID NO: 365; SEQ ID NO: 366; SEQ ID NO: 367; SEQ ID NO: 368; SEQ ID NO: 369; SEQ ID NO: 370; SEQ ID NO: 371; SEQ ID NO: 372; SEQ ID NO: 373; SEQ ID NO: 374; SEQ ID NO: 375; SEQ ID NO: 376; SEQ ID NO: 377; SEQ ID NO: 378; SEQ ID NO: 379; SEQ ID NO: 380; SEQ ID NO: 381; SEQ ID NO: 382; SEQ ID NO: 383; SEQ ID NO: 384; SEQ ID NO: 385; SEQ ID NO: 386; SEQ ID NO: 387; SEQ ID NO: 388; SEQ ID NO: 389; SEQ ID NO: 390; SEQ ID NO: 391; SEQ ID NO: 392; SEQ ID NO: 393; SEQ ID NO: 394; SEQ ID NO: 395; SEQ ID NO: 396; SEQ ID NO: 397; SEQ ID NO: 398; SEQ ID NO: 399; SEQ ID NO: 400; SEQ ID NO: 401; SEQ ID NO: 402; SEQ ID NO: 403; SEQ ID NO: 404; SEQ ID NO: 405; SEQ ID NO: 406; SEQ ID NO: 407; SEQ ID NO: 408; SEQ ID NO: 409; SEQ ID NO: 410; SEQ ID NO: 411; SEQ ID NO: 412; SEQ ID NO: 413; SEQ ID NO: 414; SEQ ID NO: 415; SEQ ID NO: 416; SEQ ID NO: 417; SEQ ID NO: 418; SEQ ID NO: 419; SEQ ID NO: 420; SEQ ID NO: 421; SEQ ID NO: 422; SEQ ID NO: 423; SEQ ID NO: 424; SEQ ID NO: 425; SEQ ID NO: 426; SEQ ID NO: 427; SEQ ID NO: 428; SEQ ID NO: 429; SEQ ID NO: 430; SEQ ID NO: 431; SEQ ID NO: 432; SEQ ID NO: 433; SEQ ID NO: 434; SEQ ID NO: 435; SEQ ID NO: 436; SEQ ID NO: 437; SEQ ID NO: 438; SEQ ID NO: 439; SEQ ID NO: 440; SEQ ID NO: 441; SEQ ID NO: 442; SEQ ID NO: 443; SEQ ID NO: 444; SEQ ID NO: 445; SEQ ID NO: 446; SEQ ID NO: 447; SEQ ID NO: 448; SEQ ID NO: 449; SEQ ID NO: 450; SEQ ID NO: 451; SEQ ID NO: 452; SEQ ID NO: 453; SEQ ID NO: 454; SEQ ID NO: 455; SEQ ID NO: 456; SEQ ID NO: 457; SEQ ID NO: 458; SEQ ID NO: 459; SEQ ID NO: 460; SEQ ID NO: 461; SEQ ID NO: 462; SEQ ID NO: 463; SEQ ID NO: 464; SEQ ID NO: 465; SEQ ID NO: 466; SEQ ID NO: 467; SEQ ID NO: 468; SEQ ID NO: 469; SEQ ID NO: 470; SEQ ID NO: 471; SEQ ID NO: 472; SEQ ID NO: 473; SEQ ID NO: 474; SEQ ID NO: 475; SEQ ID NO: 476; SEQ ID NO: 477; SEQ ID NO: 478; SEQ ID NO: 479; SEQ ID NO: 480; SEQ ID NO: 481; SEQ ID NO: 482; SEQ ID NO: 483; SEQ ID NO: 484; SEQ ID NO: 485; SEQ ID NO: 486; SEQ ID NO: 487; SEQ ID NO: 488; SEQ ID NO: 489; SEQ ID NO: 490; SEQ ID NO: 491; SEQ ID NO: 492; SEQ ID NO: 493; SEQ ID NO: 494; SEQ ID NO: 495; SEQ ID NO: 496; SEQ ID NO: 497; SEQ ID NO: 498; SEQ ID NO: 499; SEQ ID NO: 500; SEQ ID NO: 501; SEQ ID NO: 502; SEQ ID NO: 503; SEQ ID NO: 504; SEQ ID NO: 505; SEQ ID NO: 506; SEQ ID NO: 507; SEQ ID NO: 508; SEQ ID NO: 509; SEQ ID NO: 510; SEQ ID NO: 9528; SEQ ID NO: 9529; SEQ ID NO: 9530; SEQ ID NO: 9531; SEQ ID NO: 9532; SEQ ID NO: 9533; SEQ ID NO: 9534; SEQ ID NO: 9535 and SEQ ID NO: 9536.  
     [0119] In another embodiment, the  H. pylori  outer membrane polypeptide or a fragment thereof is an  H. pylori  polypeptide having a terminal phenylalanine residue or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 104; SEQ ID NO: 105; SEQ ID NO: 106; SEQ ID NO: 107; SEQ ID NO: 108; SEQ ID NO: 109; SEQ ID NO: 110; SEQ ID NO: 111; SEQ ID NO: 112; SEQ ID NO: 113; SEQ ID NO: 114; SEQ ID NO: 115; SEQ ID NO: 116; SEQ ID NO: 117; SEQ ID NO: 118; SEQ ID NO: 119; SEQ ID NO: 120; SEQ ID NO: 121; SEQ ID NO: 122; SEQ ID NO: 123; SEQ ID NO: 124; SEQ ID NO: 125; SEQ ID NO: 126; SEQ ID NO: 127; SEQ ID NO: 128; SEQ ID NO: 129; SEQ ID NO: 130; SEQ ID NO: 131; SEQ ID NO: 132; SEQ ID NO: 133; SEQ ID NO: 134; SEQ ID NO: 135; SEQ ID NO: 136; SEQ ID NO: 137; SEQ ID NO: 138; SEQ ID NO: 139; SEQ ID NO: 140; SEQ ID NO: 141; SEQ ID NO: 142; SEQ ID NO: 143; SEQ ID NO: 144; SEQ ID NO: 145; SEQ ID NO: 146; SEQ ID NO: 147; SEQ ID NO: 148; SEQ ID NO: 149; SEQ ID NO: 150; SEQ ID NO: 151; SEQ ID NO: 152; SEQ ID NO: 153; SEQ ID NO: 154; SEQ ID NO: 155; SEQ ID NO: 156; SEQ ID NO: 157; SEQ ID NO: 158; SEQ ID NO: 159; SEQ ID NO: 160; SEQ ID NO: 161; SEQ ID NO: 162; SEQ ID NO: 163; SEQ ID NO: 164; SEQ ID NO: 165; SEQ ID NO: 166; SEQ ID NO: 167; SEQ ID NO: 168; SEQ ID NO: 169; SEQ ID NO: 170; SEQ ID NO: 171; SEQ ID NO: 172; SEQ ID NO: 173; SEQ ID NO: 174; SEQ ID NO: 175; SEQ ID NO: 176; SEQ ID NO: 177; SEQ ID NO: 178; SEQ ID NO: 179; SEQ ID NO: 180; SEQ ID NO: 181; SEQ ID NO: 182; SEQ ID NO: 183; SEQ ID NO: 184; SEQ ID NO: 185; SEQ ID NO: 186; SEQ ID NO: 187; SEQ ID NO: 188; SEQ ID NO: 189; SEQ ID NO: 190, SEQ ID NO: 191; SEQ ID NO: 192; SEQ ID NO: 193; SEQ ID NO: 194; SEQ ID NO: 195; SEQ ID NO: 196; SEQ ID NO: 197; SEQ ID NO: 198; SEQ ID NO: 199; SEQ ID NO: 200; SEQ ID NO: 201; SEQ ID NO: 202; SEQ ID NO: 203; SEQ ID NO: 204; SEQ ID NO: 205; SEQ ID NO: 206; SEQ ID NO: 207; SEQ ID NO: 208; SEQ ID NO: 209; SEQ ID NO: 210; SEQ ID NO: 211; SEQ ID NO: 212; SEQ ID NO: 213; SEQ ID NO: 214; SEQ ID NO: 215; SEQ ID NO: 216; SEQ ID NO: 217; SEQ ID NO: 218; SEQ ID NO: 219; SEQ ID NO: 220; SEQ ID NO: 221; SEQ ID NO: 222; SEQ ID NO: 223; SEQ ID NO: 224; SEQ ID NO: 225; SEQ ID NO: 226; SEQ ID NO: 227; SEQ ID NO: 228; SEQ ID NO: 229; SEQ ID NO: 230; SEQ ID NO: 231; SEQ ID NO: 232; SEQ ID NO: 233; SEQ ID NO: 234; SEQ ID NO: 235; SEQ ID NO: 236; SEQ ID NO: 237; SEQ ID NO: 238; SEQ ID NO: 239; SEQ ID NO: 240; SEQ ID NO: 241; SEQ ID NO: 242; SEQ ID NO: 243; SEQ ID NO: 244; SEQ ID NO: 245; SEQ ID NO: 246; SEQ ID NO: 247; SEQ ID NO: 248; SEQ ID NO: 249; SEQ ID NO: 250; SEQ ID NO: 251; SEQ ID NO: 252; SEQ ID NO: 253; SEQ ID NO: 254; SEQ ID NO: 255; SEQ ID NO: 256; SEQ ID NO: 257; SEQ ID NO: 258; SEQ ID NO: 259; SEQ ID NO: 260; SEQ ID NO: 261; SEQ ID NO: 262; SEQ ID NO: 263; SEQ ID NO: 264; SEQ ID NO: 265; SEQ ID NO: 266; SEQ ID NO: 267; SEQ ID NO: 268; SEQ ID NO: 269; SEQ ID NO: 270; SEQ ID NO: 271; SEQ ID NO: 272; SEQ ID NO: 273; SEQ ID NO: 274; SEQ ID NO: 275; SEQ ID NO: 276; SEQ ID NO: 277; SEQ ID NO: 278; SEQ ID NO: 279; SEQ ID NO: 280; SEQ ID NO: 281; SEQ ID NO: 282; SEQ ID NO: 283; SEQ ID NO: 284; SEQ ID NO: 285; SEQ ID NO: 286; SEQ ID NO: 287; SEQ ID NO: 288; SEQ ID NO: 289; SEQ ID NO: 290; SEQ ID NO: 291; SEQ ID NO: 292; SEQ ID NO: 293; SEQ ID NO: 294; SEQ ID NO: 295; SEQ ID NO: 296; SEQ ID NO: 297; SEQ ID NO: 298; SEQ ID NO: 299; SEQ ID NO: 300; SEQ ID NO: 301; SEQ ID NO: 302; SEQ ID NO: 303; SEQ ID NO: 304; SEQ ID NO: 305; SEQ ID NO: 306; SEQ ID NO: 307; SEQ ID NO: 308; SEQ ID NO: 309; SEQ ID NO: 310; SEQ ID NO: 311; SEQ ID NO: 312; SEQ ID NO: 313; SEQ ID NO: 314; SEQ ID NO: 315; SEQ ID NO: 316; SEQ ID NO: 317; SEQ ID NO: 318; SEQ ID NO: 319; SEQ ID NO: 320; SEQ ID NO: 321; SEQ ID NO: 322; SEQ ID NO: 323; SEQ ID NO: 324; SEQ ID NO: 325 SEQ ID NO: 326; SEQ ID NO: 327; SEQ ID NO: 328; SEQ ID NO: 329; SEQ ID NO: 330; SEQ ID NO: 331; SEQ ID NO: 332; SEQ ID NO: 333; SEQ ID NO: 342; SEQ ID NO: 343; SEQ ID NO: 344; SEQ ID NO: 345; SEQ ID NO: 346; SEQ ID NO: 347; SEQ ID NO: 348; SEQ ID NO: 349; SEQ ID NO: 350; SEQ ID NO: 351; SEQ ID NO: 352; SEQ ID NO: 353; SEQ ID NO: 354; SEQ ID NO: 355; SEQ ID NO: 356; SEQ ID NO: 357; SEQ ID NO: 358; SEQ ID NO: 359; SEQ ID NO: 360; SEQ ID NO: 361; SEQ ID NO: 362; SEQ ID NO: 369; SEQ ID NO: 370; SEQ ID NO: 371; SEQ ID NO: 372; SEQ ID NO: 373; SEQ ID NO: 374; SEQ ID NO: 375; SEQ ID NO: 376; SEQ ID NO: 377; SEQ ID NO: 378; SEQ ID NO: 379; SEQ ID NO: 380; SEQ ID NO: 381; SEQ ID NO: 382; SEQ ID NO: 383; SEQ ID NO: 384; SEQ ID NO: 385; SEQ ID NO: 386; SEQ ID NO: 387; SEQ ID NO: 388; SEQ ID NO: 389; SEQ ID NO: 390; SEQ ID NO: 391; SEQ ID NO: 392; SEQ ID NO: 393; SEQ ID NO: 394; SEQ ID NO: 395; SEQ ID NO: 396; SEQ ID NO: 397; SEQ ID NO: 9528; SEQ ID NO: 9529; SEQ ID NO: 9530; SEQ ID NO: 9531; SEQ ID NO: 9532; SEQ ID NO: 9533; SEQ ID NO: 9534; SEQ ID NO: 9535 and SEQ ID NO: 9536.  
     [0120] In another embodiment, the  H. pylori  outer membrane polypeptide or a fragment thereof is an  H. pylori  polypeptide having a terminal phenylalanine residue and a C-terminal tyrosine cluster or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 326; SEQ ID NO: 327; SEQ ID NO: 328; SEQ ID NO: 329; SEQ ID NO: 330; SEQ ID NO: 331; SEQ ID NO: 332; SEQ ID NO: 333; SEQ ID NO: 342; SEQ ID NO: 343; SEQ ID NO: 344; SEQ ID NO: 345; SEQ ID NO: 346; SEQ ID NO: 347; SEQ ID NO: 348; SEQ ID NO: 349; SEQ ID NO: 350; SEQ ID NO: 351; SEQ ID NO: 352; SEQ ID NO: 353; SEQ ID NO: 354; SEQ ID NO: 355; SEQ ID NO: 356; SEQ ID NO: 357; SEQ ID NO: 358; SEQ ID NO: 359; SEQ ID NO: 360; SEQ ID NO: 361; SEQ ID NO: 362; SEQ ID NO: 369; SEQ ID NO: 370; SEQ ID NO: 371; SEQ ID NO: 372; SEQ ID NO: 373; SEQ ID NO: 374; SEQ ID NO: 375; SEQ ID NO: 376; SEQ ID NO: 377; SEQ ID NO: 378; SEQ ID NO: 379; SEQ ID NO: 380; SEQ ID NO: 381; SEQ ID NO: 382; SEQ ID NO: 383; SEQ ID NO: 384; SEQ ID NO: 385; SEQ ID NO: 386; SEQ ID NO: 387; SEQ ID NO: 388; SEQ ID NO: 389; SEQ ID NO: 390; SEQ ID NO: 391; SEQ ID NO: 392; SEQ ID NO: 393; SEQ ID NO: 394; SEQ ID NO: 395; SEQ ID NO: 396; SEQ ID NO: 397 and SEQ ID NO: 9536.  
     [0121] In another embodiment, the  H. pylori  outer membrane polypeptide or a fragment thereof is an  H. pylori  polypeptide having a terminal tyrosine cluster motif or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 326; SEQ ID NO: 327; SEQ ID NO: 328; SEQ ID NO: 329; SEQ ID NO: 330; SEQ ID NO: 331; SEQ ID NO: 332; SEQ ID NO: 333; SEQ ID NO: 334; SEQ ID NO: 335; SEQ ID NO: 336; SEQ ID NO: 337; SEQ ID NO: 338; SEQ ID NO: 339; SEQ ID NO: 340; SEQ ID NO: 341; SEQ ID NO: 342; SEQ ID NO: 343; SEQ ID NO: 344; SEQ ID NO: 345; SEQ ID NO: 346; SEQ ID NO: 347; SEQ ID NO: 348; SEQ ID NO: 349; SEQ ID NO: 350; SEQ ID NO: 351; SEQ ID NO: 352; SEQ ID NO: 353; SEQ ID NO: 354; SEQ ID NO: 355; SEQ ID NO: 356; SEQ ID NO: 357; SEQ ID NO: 358; SEQ ID NO: 359; SEQ ID NO: 360; SEQ ID NO: 361; SEQ ID NO: 362; SEQ ID NO: 363; SEQ ID NO: 364; SEQ ID NO: 365; SEQ ID NO: 366; SEQ ID NO: 367; SEQ ID NO: 368; SEQ ID NO: 369; SEQ ID NO: 370; SEQ ID NO: 371; SEQ ID NO: 372; SEQ ID NO: 373; SEQ ID NO: 374; SEQ ID NO: 375; SEQ ID NO: 376; SEQ ID NO: 377; SEQ ID NO: 378; SEQ ID NO: 379; SEQ ID NO: 380; SEQ ID NO: 381; SEQ ID NO: 382; SEQ ID NO: 383; SEQ ID NO: 384; SEQ ID NO: 385; SEQ ID NO: 386; SEQ ID NO: 387; SEQ ID NO: 388; SEQ ID NO: 389; SEQ ID NO: 390; SEQ ID NO: 391; SEQ ID NO: 392; SEQ ID NO: 393; SEQ ID NO: 394; SEQ ID NO: 395; SEQ ID NO: 396; SEQ ID NO: 397; SEQ ID NO: 398; SEQ ID NO: 399; SEQ ID NO: 400; SEQ ID NO: 401; SEQ ID NO: 402; SEQ ID NO: 403; SEQ ID NO: 404; SEQ ID NO: 405; SEQ ID NO: 406; SEQ ID NO: 407; SEQ ID NO: 408; SEQ ID NO: 409; SEQ ID NO: 410; SEQ ID NO: 411; SEQ ID NO: 412; SEQ ID NO: 413; SEQ ID NO: 414; SEQ ID NO: 415; SEQ ID NO: 416; SEQ ID NO: 424; SEQ ID NO: 425 and SEQ ID NO: 9536.  
     [0122] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 511; SEQ ID NO: 512; SEQ ID NO: 513; SEQ ID NO: 514; SEQ ID NO: 515; SEQ ID NO: 516; SEQ ID NO: 517; SEQ ID NO: 518; SEQ ID NO: 519; SEQ ID NO: 520; SEQ ID NO: 521; SEQ ID NO: 522; SEQ ID NO: 523; SEQ ID NO: 524; SEQ ID NO: 525; SEQ ID NO: 526; SEQ ID NO: 527; SEQ ID NO: 528; SEQ ID NO: 529; SEQ ID NO: 530; SEQ ID NO: 531; SEQ ID NO: 532; SEQ ID NO: 533; SEQ ID NO: 534; SEQ ID NO: 535; SEQ ID NO: 536; SEQ ID NO: 537; SEQ ID NO: 538; SEQ ID NO: 539; SEQ ID NO: 540; SEQ ID NO: 541; SEQ ID NO: 542; SEQ ID NO: 543; SEQ ID NO: 544; SEQ ID NO: 545; SEQ ID NO: 546; SEQ ID NO: 547; SEQ ID NO: 548; SEQ ID NO: 549; SEQ ID NO: 550; SEQ ID NO: 551; SEQ ID NO: 552; SEQ ID NO: 553; SEQ ID NO: 554; SEQ ID NO: 555; SEQ ID NO: 556; SEQ ID NO: 557; SEQ ID NO: 558; SEQ ID NO: 559; SEQ ID NO: 560; SEQ ID NO: 561; SEQ ID NO: 562; SEQ ID NO: 563; SEQ ID NO: 564; SEQ ID NO: 565; SEQ ID NO: 566; SEQ ID NO: 567; SEQ ID NO: 568; SEQ ID NO: 569; SEQ ID NO: 570; SEQ ID NO: 571; SEQ ID NO: 572; SEQ ID NO: 573; SEQ ID NO: 574; SEQ ID NO: 575; SEQ ID NO: 576; SEQ ID NO: 577; SEQ ID NO: 578; SEQ ID NO: 579; SEQ ID NO: 580; SEQ ID NO: 581; SEQ ID NO: 582; SEQ ID NO: 583; SEQ ID NO: 584; SEQ ID NO: 585; SEQ ID NO: 586; SEQ ID NO: 587; SEQ ID NO: 588; SEQ ID NO: 589; SEQ ID NO: 590; SEQ ID NO: 591; SEQ ID NO: 592; SEQ ID NO: 593; SEQ ID NO: 594; SEQ ID NO: 595; SEQ ID NO: 596; SEQ ID NO: 597; SEQ ID NO: 598; SEQ ID NO: 599; SEQ ID NO: 600; SEQ ID NO: 601; SEQ ID NO: 602; SEQ ID NO: 603; SEQ ID NO: 604; SEQ ID NO: 605; SEQ ID NO: 606; SEQ ID NO: 607; SEQ ID NO: 608; SEQ ID NO: 609; SEQ ID NO: 610; SEQ ID NO: 611; SEQ ID NO: 612; SEQ ID NO: 613; SEQ ID NO: 614; SEQ ID NO: 615; SEQ ID NO: 616; SEQ ID NO: 617; SEQ ID NO: 618; SEQ ID NO: 619; SEQ ID NO: 620; SEQ ID NO: 621; SEQ ID NO: 622; SEQ ID NO: 623; SEQ ID NO: 624; SEQ ID NO: 625; SEQ ID NO: 626; SEQ ID NO: 627; SEQ ID NO: 628; SEQ ID NO: 629; SEQ ID NO: 630; SEQ ID NO: 631; SEQ ID NO: 632; SEQ ID NO: 633; SEQ ID NO: 634; SEQ ID NO: 635; SEQ ID NO: 636; SEQ ID NO: 637; SEQ ID NO: 638; SEQ ID NO: 639; SEQ ID NO: 640; SEQ ID NO: 641; SEQ ID NO: 642; SEQ ID NO: 643; SEQ ID NO: 644; SEQ ID NO: 645; SEQ ID NO: 646; SEQ ID NO: 647; SEQ ID NO: 648; SEQ ID NO: 649; SEQ ID NO: 650; SEQ ID NO: 651; SEQ ID NO: 652; SEQ ID NO: 653; SEQ ID NO: 654; SEQ ID NO: 655; SEQ ID NO: 656; SEQ ID NO: 657; SEQ ID NO: 658; SEQ ID NO: 659; SEQ ID NO: 660; SEQ ID NO: 661; SEQ ID NO: 662; SEQ ID NO: 663; SEQ ID NO: 664; SEQ ID NO: 665; SEQ ID NO: 666; SEQ ID NO: 667; SEQ ID NO: 668; SEQ ID NO: 669; SEQ ID NO: 670; SEQ ID NO: 671; SEQ ID NO: 672; SEQ ID NO: 673; SEQ ID NO: 674; SEQ ID NO: 675; SEQ ID NO: 676; SEQ ID NO: 677; SEQ ID NO: 678; SEQ ID NO: 678; SEQ ID NO: 680; SEQ ID NO: 681; SEQ ID NO: 682; SEQ ID NO: 683; SEQ ID NO: 684; SEQ ID NO: 685; SEQ ID NO: 686; SEQ ID NO: 687; SEQ ID NO: 688; SEQ ID NO: 689; SEQ ID NO: 690; SEQ ID NO: 691; SEQ ID NO: 692; SEQ ID NO: 693; SEQ ID NO: 694; SEQ ID NO: 695; SEQ ID NO: 696; SEQ ID NO: 697; SEQ ID NO: 698; SEQ ID NO: 699; SEQ ID NO: 700; SEQ ID NO: 701; SEQ ID NO: 702; SEQ ID NO: 703; SEQ ID NO: 704; SEQ ID NO: 705; SEQ ID NO: 706; SEQ ID NO: 707; SEQ ID NO: 708; SEQ ID NO: 709; SEQ ID NO: 710; SEQ ID NO: 711; SEQ ID NO: 712; SEQ ID NO: 713; SEQ ID NO: 714; SEQ ID NO: 715; SEQ ID NO: 716; SEQ ID NO: 717; SEQ ID NO: 718; SEQ ID NO: 719; SEQ ID NO: 720; SEQ ID NO: 721; SEQ ID NO: 722; SEQ ID NO: 723; SEQ ID NO: 724; SEQ ID NO: 725; SEQ ID NO: 726; SEQ ID NO: 727; SEQ ID NO: 728; SEQ ID NO: 729; SEQ ID NO: 730; SEQ ID NO: 731; SEQ ID NO: 732; SEQ ID NO: 733; SEQ ID NO: 734; SEQ ID NO: 735; SEQ ID NO: 736; SEQ ID NO: 737; SEQ ID NO: 738; SEQ ID NO: 739; SEQ ID NO: 740; SEQ ID NO: 741; SEQ ID NO: 742; SEQ ID NO: 743; SEQ ID NO: 744; SEQ ID NO: 745; SEQ ID NO: 746; SEQ ID NO: 747; SEQ ID NO: 748; SEQ ID NO: 749; SEQ ID NO: 750; SEQ ID NO: 751; SEQ ID NO: 752; SEQ ID NO: 753; SEQ ID NO: 754; SEQ ID NO: 755; SEQ ID NO: 756; SEQ ID NO: 757; SEQ ID NO: 758; SEQ ID NO: 759; SEQ ID NO: 760; SEQ ID NO: 761; SEQ ID NO: 762; SEQ ID NO: 763; SEQ ID NO: 764; SEQ ID NO: 765; SEQ ID NO: 766; SEQ ID NO: 767; SEQ ID NO: 768; SEQ ID NO: 769; SEQ ID NO: 770; SEQ ID NO: 771; SEQ ID NO: 772; SEQ ID NO: 773; SEQ ID NO: 774; SEQ ID NO: 775; SEQ ID NO: 776; SEQ ID NO: 777; SEQ ID NO: 778; SEQ ID NO: 779; SEQ ID NO: 780; SEQ ID NO: 781; SEQ ID NO: 782; SEQ ID NO: 783; SEQ ID NO: 784; SEQ ID NO: 785; SEQ ID NO: 786; SEQ ID NO: 787; SEQ ID NO: 788; SEQ ID NO: 789; SEQ ID NO: 790; SEQ ID NO: 791; SEQ ID NO: 792; SEQ ID NO: 793; SEQ ID NO: 794; SEQ ID NO: 795; SEQ ID NO: 796; SEQ ID NO: 797; SEQ ID NO: 798; SEQ ID NO: 799; SEQ ID NO: 800; SEQ ID NO: 801; SEQ ID NO: 802; SEQ ID NO: 803; SEQ ID NO: 804; SEQ ID NO: 805; SEQ ID NO: 806; SEQ ID NO: 807; SEQ ID NO: 808; SEQ ID NO: 809; SEQ ID NO: 810; SEQ ID NO: 811; SEQ ID NO: 812; SEQ ID NO: 813; SEQ ID NO: 814; SEQ ID NO: 815; SEQ ID NO: 816; SEQ ID NO: 817; SEQ ID NO: 818; SEQ ID NO: 819; SEQ ID NO: 820; SEQ ID NO: 821; SEQ ID NO: 822; SEQ ID NO: 823; SEQ ID NO: 824; SEQ ID NO: 825; SEQ ID NO: 826; SEQ ID NO: 827; SEQ ID NO: 828; SEQ ID NO: 829; SEQ ID NO: 830; SEQ ID NO: 831; SEQ ID NO: 832; SEQ ID NO: 833; SEQ ID NO: 834; SEQ ID NO: 835; SEQ ID NO: 836; SEQ ID NO: 837; SEQ ID NO: 838; SEQ ID NO: 839; SEQ ID NO: 840; SEQ ID NO: 841; SEQ ID NO: 842; SEQ ID NO: 843; SEQ ID NO: 844; SEQ ID NO: 845; SEQ ID NO: 846; SEQ ID NO: 847; SEQ ID NO: 848; SEQ ID NO: 849; SEQ ID NO: 850; SEQ ID NO: 851; SEQ ID NO: 852; SEQ ID NO: 853; SEQ ID NO: 854; SEQ ID NO: 855; SEQ ID NO: 856; SEQ ID NO: 857; SEQ ID NO: 858; SEQ ID NO: 859; SEQ ID NO: 860; SEQ ID NO: 861; SEQ ID NO: 862; SEQ ID NO: 863; SEQ ID NO: 864; SEQ ID NO: 865; SEQ ID NO: 866; SEQ ID NO: 867; SEQ ID NO: 868; SEQ ID NO: 869; SEQ ID NO: 870; SEQ ID NO: 871; SEQ ID NO: 872; SEQ ID NO: 873; SEQ ID NO: 874; SEQ ID NO: 875; SEQ ID NO: 876; SEQ ID NO: 877; SEQ ID NO: 878; SEQ ID NO: 879; SEQ ID NO: 880; SEQ ID NO: 881; SEQ ID NO: 882; SEQ ID NO: 883; SEQ ID NO: 884; SEQ ID NO: 885; SEQ ID NO: 886; SEQ ID NO: 887; SEQ ID NO: 888; SEQ ID NO: 889; SEQ ID NO: 890; SEQ ID NO: 891; SEQ ID NO: 892; SEQ ID NO: 893; SEQ ID NO: 894; SEQ ID NO: 895; SEQ ID NO: 896; SEQ ID NO: 897; SEQ ID NO: 898; SEQ ID NO: 899; SEQ ID NO: 900; SEQ ID NO: 901; SEQ ID NO: 902; SEQ ID NO: 903; SEQ ID NO: 904; SEQ ID NO: 905; SEQ ID NO: 906; SEQ ID NO: 907; SEQ ID NO: 908; SEQ ID NO: 909; SEQ ID NO: 910; SEQ ID NO: 911; SEQ ID NO: 912; SEQ ID NO: 913; SEQ ID NO: 914; SEQ ID NO: 915; SEQ ID NO: 916; SEQ ID NO: 917; SEQ ID NO: 918; SEQ ID NO: 919; SEQ ID NO: 920; SEQ ID NO: 921; SEQ ID NO: 922; SEQ ID NO: 923; SEQ ID NO: 924; SEQ ID NO: 925; SEQ ID NO: 926; SEQ ID NO: 927; SEQ ID NO: 928; SEQ ID NO: 929; SEQ ID NO: 930; SEQ ID NO: 931; SEQ ID NO: 932; SEQ ID NO: 933; SEQ ID NO: 934; SEQ ID NO: 935; SEQ ID NO: 936; SEQ ID NO: 937; SEQ ID NO: 938; SEQ ID NO: 939; SEQ ID NO: 940; SEQ ID NO: 941; SEQ ID NO: 942; SEQ ID NO: 943; SEQ ID NO: 944; SEQ ID NO: 945; SEQ ID NO: 946; SEQ ID NO: 947; SEQ ID NO: 948; SEQ ID NO: 949; SEQ ID NO: 950; SEQ ID NO: 951; SEQ ID NO: 952; SEQ ID NO: 953; SEQ ID NO: 954; SEQ ID NO: 955; SEQ ID NO: 956; SEQ ID NO: 957; SEQ ID NO: 958; SEQ ID NO: 959; SEQ ID NO: 960; SEQ ID NO: 961; SEQ ID NO: 962; SEQ ID NO: 963; SEQ ID NO: 964; SEQ ID NO: 965; SEQ ID NO: 966; SEQ ID NO: 967; SEQ ID NO: 968; SEQ ID NO: 969; SEQ ID NO: 970; SEQ ID NO: 971; SEQ ID NO: 972; SEQ ID NO: 973; SEQ ID NO: 974; SEQ ID NO: 975; SEQ ID NO: 976; SEQ ID NO: 977; SEQ ID NO: 978; SEQ ID NO: 979; SEQ ID NO: 980; SEQ ID NO: 981; SEQ ID NO: 982; SEQ ID NO: 983; SEQ ID NO: 984; SEQ ID NO: 985; SEQ ID NO: 986; SEQ ID NO: 987; SEQ ID NO: 988; SEQ ID NO: 989; SEQ ID NO: 990; SEQ ID NO: 991; SEQ ID NO: 992; SEQ ID NO: 993; SEQ ID NO: 994; SEQ ID NO: 995; SEQ ID NO: 996; SEQ ID NO: 997; SEQ ID NO: 998; SEQ ID NO: 999; SEQ ID NO: 1000; SEQ ID NO: 1001; SEQ ID NO: 1002; SEQ ID NO: 1003; SEQ ID NO: 1004; SEQ ID NO: 1005; SEQ ID NO: 1006; SEQ ID NO: 1007; SEQ ID NO: 1008; SEQ ID NO: 1009; SEQ ID NO: 1010; SEQ ID NO: 1011; SEQ ID NO: 1012; SEQ ID NO: 1013; SEQ ID NO: 1014; SEQ ID NO: 1015; SEQ ID NO: 1016; SEQ ID NO: 1017; SEQ ID NO: 1018; SEQ ID NO: 1019; SEQ ID NO: 1020; SEQ ID NO: 1021; SEQ ID NO: 1022; SEQ ID NO: 1023; SEQ ID NO: 1024; SEQ ID NO: 1025; SEQ ID NO: 1026; SEQ ID NO: 1027; SEQ ID NO: 1028; SEQ ID NO: 1029; SEQ ID NO: 1030; SEQ ID NO: 1031; SEQ ID NO: 1032; SEQ ID NO: 1033; SEQ ID NO: 1034; SEQ ID NO: 1035; SEQ ID NO: 1036; SEQ ID NO: 1037; SEQ ID NO: 1038; SEQ ID NO: 1039; SEQ ID NO: 1040; SEQ ID NO: 1041; SEQ ID NO: 1042; SEQ ID NO: 1043; SEQ ID NO: 1044; SEQ ID NO: 1045; SEQ ID NO: 1046; SEQ ID NO: 1047; SEQ ID NO: 1048; SEQ ID NO: 1049; SEQ ID NO: 1050; SEQ ID NO: 1051; SEQ ID NO: 1052; SEQ ID NO: 1053; SEQ ID NO: 1054; SEQ ID NO: 1055; SEQ ID NO: 1056; SEQ ID NO: 1057; SEQ ID NO: 1058; SEQ ID NO: 1059; SEQ ID NO: 1060; SEQ ID NO: 1061; SEQ ID NO: 1062; SEQ ID NO: 1063; SEQ ID NO: 1064; SEQ ID NO: 1065; SEQ ID NO: 1066; SEQ ID NO: 1067; SEQ ID NO: 1068; SEQ ID NO: 1069; SEQ ID NO: 1070; SEQ ID NO: 1071; SEQ ID NO: 1072; SEQ ID NO: 1073; SEQ ID NO: 1074; SEQ ID NO: 1075; SEQ ID NO: 1076; SEQ ID NO: 1077; SEQ ID NO: 1078; SEQ ID NO: 1079; SEQ ID NO: 1080; SEQ ID NO: 1081; SEQ ID NO: 1082; SEQ ID NO: 1083; SEQ ID NO: 1084; SEQ ID NO: 1085; SEQ ID NO: 1086; SEQ ID NO: 1087; SEQ ID NO: 1088; SEQ ID NO: 1089; SEQ ID NO: 1090; SEQ ID NO: 1091; SEQ ID NO: 1092; SEQ ID NO: 1093; SEQ ID NO: 1094; SEQ ID NO: 1095; SEQ ID NO: 1096; SEQ ID NO: 1097; SEQ ID NO: 1098; SEQ ID NO: 1099; SEQ ID NO: 1100; SEQ ID NO: 1101; SEQ ID NO: 1102; SEQ ID NO: 1103; SEQ ID NO: 1104; SEQ ID NO: 1105; SEQ ID NO: 1106; SEQ ID NO: 1107; SEQ ID NO: 1108; SEQ ID NO: 1109; SEQ ID NO: 1110; SEQ ID NO: 1111; SEQ ID NO: 1112; SEQ ID NO: 1113; SEQ ID NO: 1114; SEQ ID NO: 1115; SEQ ID NO: 1116; SEQ ID NO: 1117; SEQ ID NO: 1118; SEQ ID NO: 1119; SEQ ID NO: 1120; SEQ ID NO: 1121; SEQ ID NO: 1122; SEQ ID NO: 1123; SEQ ID NO: 1124; SEQ ID NO: 1125; SEQ ID NO: 1126; SEQ ID NO: 1127; SEQ ID NO: 1128; SEQ ID NO: 1129; SEQ ID NO: 1130; SEQ ID NO: 1131; SEQ ID NO: 1132; SEQ ID NO: 1133; SEQ ID NO: 1134; SEQ ID NO: 1135; SEQ ID NO: 1136; SEQ ID NO: 1137; SEQ ID NO: 1138; SEQ ID NO: 1139; SEQ ID NO: 1140; SEQ ID NO: 1141; SEQ ID NO: 1142; SEQ ID NO: 1143; SEQ ID NO: 1144; SEQ ID NO: 1145; SEQ ID NO: 1146; SEQ ID NO: 1147; SEQ ID NO: 1148; SEQ ID NO: 1149; SEQ ID NO: 1150; SEQ ID NO: 1151; SEQ ID NO: 1152; SEQ ID NO: 1153; SEQ ID NO: 1154; SEQ ID NO: 1155; SEQ ID NO: 1156; SEQ ID NO: 1157; SEQ ID NO: 1158; SEQ ID NO: 1159; SEQ ID NO: 1160; SEQ ID NO: 1161; SEQ ID NO: 1162; SEQ ID NO: 1163; SEQ ID NO: 1164; SEQ ID NO: 1165; SEQ ID NO: 1166; SEQ ID NO: 1167; SEQ ID NO: 1168; SEQ ID NO: 1169; SEQ ID NO: 1170; SEQ ID NO: 1171; SEQ ID NO: 1172; SEQ ID NO: 1173; SEQ ID NO: 1174; SEQ ID NO: 1175; SEQ ID NO: 1176; SEQ ID NO: 1177; SEQ ID NO: 1178; SEQ ID NO: 1179; SEQ ID NO: 1180; SEQ ID NO: 1181; SEQ ID NO: 1182; SEQ ID NO: 1183; SEQ ID NO: 1184; SEQ ID NO: 1185; SEQ ID NO: 1186; SEQ ID NO: 1187; SEQ ID NO: 1188; SEQ ID NO: 1189; SEQ ID NO: 1190; SEQ ID NO: 1191; SEQ ID NO: 1192; SEQ. ID NO: 1193; SEQ ID NO: 1194; SEQ ID NO: 1195; SEQ ID NO: 1196; SEQ ID NO: 119; SEQ ID NO: 1198; SEQ ID NO: 1199; SEQ ID NO: 1200; SEQ ID NO: 1201; SEQ ID NO: 1202; SEQ ID NO: 1203; SEQ ID NO: 1204; SEQ ID NO: 1205; SEQ ID NO: 1206; SEQ ID NO: 1207; SEQ ID NO: 1208; SEQ ID NO: 1209; SEQ ID NO: 1210; SEQ ID NO:  121 I; SEQ ID NO: 1212; SEQ ID NO: 1213; SEQ ID NO: 1214; SEQ ID NO: 1215; SEQ ID NO: 1216; SEQ ID NO: 1217; SEQ ID NO: 1218; SEQ ID NO: 1219; SEQ ID NO: 1220; SEQ ID NO: 1221; SEQ ID NO: 1222; SEQ ID NO: 1223; SEQ ID NO: 1224; SEQ ID NO: 1225; SEQ ID NO: 1226; SEQ ID NO: 1227; SEQ ID NO: 1228; SEQ ID NO: 1229; SEQ ID NO: 1230; SEQ ID NO: 1231; SEQ ID NO: 1232; SEQ ID NO: 1233; SEQ ID NO: 1234; SEQ ID NO: 1235; SEQ ID NO: 1236; SEQ ID NO: 1237; SEQ ID NO: 1238; SEQ ID NO: 1239; SEQ ID NO: 1240; SEQ ID NO: 1241; SEQ ID NO: 1242; SEQ ID NO: 1243; SEQ ID NO: 1244; SEQ ID NO: 1245; SEQ ID NO: 1246; SEQ ID NO: 1247; SEQ ID NO: 1248; SEQ ID NO: 1249; SEQ ID NO: 1250; SEQ ID NO: 1251; SEQ ID NO: 1252; SEQ ID NO: 1253; SEQ ID NO: 1254; SEQ ID NO: 1255; SEQ ID NO: 1256; SEQ ID NO: 1257; SEQ ID NO: 1258; SEQ ID NO: 1259; SEQ ID NO: 1260; SEQ ID NO: 1261; SEQ ID NO: 1262; SEQ ID NO: 1263; SEQ ID NO: 1264; SEQ ID NO: 1265; SEQ ID NO: 1266; SEQ ID NO: 1267; SEQ ID NO: 1268; SEQ ID NO: 1269; SEQ ID NO: 1270; SEQ ID NO: 1271; SEQ ID NO: 1272; SEQ ID NO: 1273; SEQ ID NO: 1274; SEQ ID NO: 1275; SEQ ID NO: 1276; SEQ ID NO: 1277; SEQ ID NO: 1278; SEQ ID NO: 1279; SEQ ID NO: 1280; SEQ ID NO: 1281; SEQ ID NO: 1282; SEQ ID NO: 1283; SEQ ID NO: 1284; SEQ ID NO: 1285; SEQ ID NO: 1286; SEQ ID NO: 1287; SEQ ID NO: 1288; SEQ ID NO: 1289; SEQ ID NO: 1290; SEQ ID NO: 1291; SEQ ID NO: 1292; SEQ ID NO: 1293; SEQ ID NO: 1294; SEQ ID NO: 1295; SEQ ID NO: 1296; SEQ ID NO: 1297; SEQ ID NO: 1298; SEQ ID NO: 1299; SEQ ID NO: 1300; SEQ ID NO: 1301; SEQ ID NO: 1302; SEQ ID NO: 1303; SEQ ID NO: 1304; SEQ ID NO: 1305; SEQ ID NO: 1306; SEQ ID NO: 1307; SEQ ID NO: 1308; SEQ ID NO: 1309; SEQ ID NO: 1310; SEQ ID NO:  131 I; SEQ ID NO: 1312; SEQ ID NO:1313; SEQ ID NO: 1314; SEQ ID NO: 1315; SEQ ID NO: 1316; SEQ ID NO: 1317; SEQ ID NO: 1318; SEQ ID NO: 1319; SEQ ID NO: 1320; SEQ ID NO: 1321; SEQ ID NO: 1322; SEQ ID NO: 1323; SEQ ID NO: 1324; SEQ ID NO: 1325; SEQ ID NO: 1326; SEQ ID NO: 1327; SEQ ID NO: 1328; SEQ ID NO: 1329; SEQ ID NO: 1330; SEQ ID NO: 1331; SEQ ID NO: 1332; SEQ ID NO: 1333; SEQ ID NO: 1334; SEQ ID NO: 1335; SEQ ID NO: 1336; SEQ ID NO: 1337; SEQ ID NO: 1338; SEQ ID NO: 1339; SEQ ID NO: 1340; SEQ ID NO: 1341; SEQ ID NO: 1342; SEQ ID NO: 1343; SEQ ID NO: 1344; SEQ ID NO: 1345; SEQ ID NO: 1346; SEQ ID NO: 1347; SEQ ID NO: 1348; SEQ ID NO: 1349; SEQ ID NO: 1350; SEQ ID NO: 1351; SEQ ID NO: 1352; SEQ ID NO: 1353; SEQ ID NO: 1354; SEQ ID NO: 1355; SEQ ID NO: 1356; SEQ ID NO: 1357; SEQ ID NO: 1358; SEQ ID NO: 1359; SEQ ID NO: 1360; SEQ ID NO: 1361; SEQ ID NO: 1362; SEQ ID NO: 1363; SEQ ID NO: 1364; SEQ ID NO: 1365; SEQ ID NO: 1366; SEQ ID NO: 1367; SEQ ID NO: 1368; SEQ ID NO: 1369; SEQ ID NO: 1370; SEQ ID NO: 1371; SEQ ID NO: 1372; SEQ ID NO: 1373; SEQ ID NO: 1374; SEQ ID NO: 1375; SEQ ID NO: 1376; SEQ ID NO: 1377; SEQ ID NO: 1378; SEQ ID NO: 1379; SEQ ID NO: 1380; SEQ ID NO: 1381; SEQ ID NO: 1382; SEQ ID NO: 1383; SEQ ID NO: 1384; SEQ ID NO: 1385; SEQ ID NO: 1386; SEQ ID NO: 1387; SEQ ID NO: 1388; SEQ ID NO: 1389; SEQ ID NO: 1390; SEQ ID NO: 1391; SEQ ID NO: 1392; SEQ ID NO: 1393; SEQ ID NO: 1394; SEQ ID NO: 1395; SEQ ID NO: 1396; SEQ ID NO: 1397; SEQ ID NO: 1398; SEQ ID NO: 1399; SEQ ID NO: 1400; SEQ ID NO: 1401; SEQ ID NO: 1402; SEQ ID NO: 1403; SEQ ID NO: 1404; SEQ ID NO: 1405; SEQ ID NO: 1406; SEQ ID NO: 1407; SEQ ID NO: 1408; SEQ ID NO: 1409; SEQ ID NO: 1410; SEQ ID NO: 1411; SEQ ID NO: 1412; SEQ ID NO: 1413; SEQ ID NO: 1414; SEQ ID NO: 1415; SEQ ID NO: 1416; SEQ ID NO: 1417; SEQ ID NO: 1418; SEQ ID NO: 1419; SEQ ID NO: 1420; SEQ ID NO: 1421; SEQ ID NO: 1422; SEQ ID NO: 1423; SEQ ID NO: 1424; SEQ ID NO: 1425; SEQ ID NO: 1426; SEQ ID NO: 1427; SEQ ID NO: 1428; SEQ ID NO: 1429; SEQ ID NO: 1430; SEQ ID NO: 1431; SEQ ID NO: 1432; SEQ ID NO: 1433; SEQ ID NO: 1434; SEQ ID NO: 1435; SEQ ID NO: 1436; SEQ ID NO: 1437; SEQ ID NO: 1438; SEQ ID NO: 1439; SEQ ID NO: 1440; SEQ ID NO: 1441; SEQ ID NO: 1442; SEQ ID NO: 1443; SEQ ID NO: 1444; SEQ ID NO: 1445; SEQ ID NO: 1446; SEQ ID NO: 1447; SEQ ID NO: 1448; SEQ ID NO: 1449; SEQ ID NO: 1450; SEQ ID NO: 1451; SEQ ID NO: 1452; SEQ ID NO: 1453; SEQ ID NO: 1454; SEQ ID NO: 1455; SEQ ID NO: 1456; SEQ ID NO: 1457; SEQ ID NO: 1458; SEQ ID NO: 1459; SEQ ID NO: 1460; SEQ ID NO: 1461; SEQ ID NO: 1462; SEQ ID NO: 1463; SEQ ID NO: 1464; SEQ ID NO: 1465; SEQ ID NO: 1466; SEQ ID NO: 1467; SEQ ID NO: 1468; SEQ ID NO: 1469; SEQ ID NO: 1470; SEQ ID NO: 1471; SEQ ID NO: 1472; SEQ ID NO: 1473; SEQ ID NO: 1474; SEQ ID NO: 1475; SEQ ID NO: 1476; SEQ ID NO: 1477; SEQ ID NO: 1478;-SEQ ID NO: 1479; SEQ ID NO: 1480; SEQ ID NO: 1481; SEQ ID NO: 1482; SEQ ID NO: 1483; SEQ ID NO: 1484; SEQ ID NO: 1485; SEQ ID NO: 1486; SEQ ID NO: 1487; SEQ ID NO: 1488; SEQ ID NO: 1489; SEQ ID NO: 1490; SEQ ID NO: 1491; SEQ ID NO: 1492; SEQ ID NO: 1493; SEQ ID NO: 1494; SEQ ID NO: 1495; SEQ ID NO: 1496; SEQ ID NO: 1497; SEQ ID NO: 1498; SEQ ID NO: 1499; SEQ ID NO: 1500; SEQ ID NO: 1501; SEQ ID NO: 1502; SEQ ID NO: 1503; SEQ ID NO: 1504; SEQ ID NO: 1505; SEQ ID NO: 1506; SEQ ID NO: 1507; SEQ ID NO: 1508; SEQ ID NO: 1509; SEQ ID NO: 1510; SEQ ID NO: 1511; SEQ ID NO: 1512; SEQ ID NO: 1513; SEQ ID NO: 1514; SEQ ID NO: 9537; SEQ ID NO: 9538; SEQ ID NO: 9539; SEQ ID NO: 9540; SEQ ID NO: 9541; SEQ ID NO: 9542; SEQ ID NO: 9543; SEQ ID NO: 9544; SEQ ID NO: 9545; SEQ ID NO: 9546; SEQ ID NO: 9547; SEQ ID NO: 9548; SEQ ID NO: 9549; SEQ ID NO: 9550; SEQ ID NO: 9551; SEQ ID NO: 9552; SEQ ID NO: 9553; SEQ ID NO: 9554; SEQ ID NO: 9555; SEQ ID NO: 9556; SEQ ID NO: 9557; SEQ ID NO: 9558; SEQ ID NO: 9559; SEQ ID NO: 9560; SEQ ID NO: 9561; SEQ ID NO: 9562; SEQ ID NO: 9563; SEQ ID NO: 9564; SEQ ID NO: 9565; SEQ ID NO: 9566; SEQ ID NO: 9567; SEQ ID NO: 9568; SEQ ID NO: 9569; SEQ ID NO: 9570; SEQ ID NO: 9571; SEQ ID NO: 9572; SEQ ID NO: 9573; SEQ ID NO: 9574; SEQ ID NO: 9575; SEQ ID NO: 9576; SEQ ID NO: 9577; SEQ ID NO: 9578; SEQ ID NO: 9579; SEQ ID NO: 9580; SEQ ID NO: 9581; SEQ ID NO: 9582; SEQ ID NO: 9583; SEQ ID NO: 9584; SEQ ID NO: 9585; SEQ ID NO: 9586; SEQ ID NO: 9587; SEQ ID NO: 9588; SEQ ID NO: 9589; SEQ ID NO: 9590 and SEQ ID NO:9591.  
     [0123] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in transport encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 511; SEQ ID NO: 512; SEQ ID NO: 513; SEQ ID NO: 514; SEQ ID NO:515; SEQ ID NO:516; SEQ ID NO:517; SEQ ID NO:518; SEQ ID NO: 519; SEQ ID NO: 520; SEQ ID NO: 521; SEQ ID NO: 522; SEQ ID NO: 523; SEQ ID NO: 524; SEQ ID NO: 525; SEQ ID NO: 526; SEQ ID NO: 527; SEQ ID NO: 528; SEQ ID NO: 529; SEQ ID NO: 530; SEQ ID NO: 531; SEQ ID NO: 532; SEQ ID NO: 533; SEQ ID NO: 534; SEQ ID NO: 535; SEQ ID NO: 536; SEQ ID NO: 537; SEQ ID NO: 538; SEQ ID NO: 539; SEQ ID NO: 540; SEQ ID NO: 541; SEQ ID NO: 542; SEQ ID NO: 543; SEQ ID NO: 544; SEQ ID NO: 545; SEQ ID NO: 546; SEQ ID NO: 547; SEQ ID NO: 548; SEQ ID NO: 549; SEQ ID NO: 550; SEQ ID NO: 551; SEQ ID NO: 552; SEQ ID NO: 553; SEQ ID NO: 554; SEQ ID NO: 555; SEQ ID NO: 556; SEQ ID NO: 557; SEQ ID NO: 558; SEQ ID NO: 559; SEQ ID NO: 560; SEQ ID NO: 561; SEQ ID NO: 562; SEQ ID NO: 563; SEQ ID NO: 564; SEQ ID NO: 565; SEQ ID NO: 566; SEQ ID NO: 567; SEQ ID NO: 568; SEQ ID NO: 569; SEQ ID NO: 570; SEQ ID NO: 571; SEQ ID NO: 572; SEQ ID NO: 573; SEQ ID NO: 574; SEQ ID NO: 575; SEQ ID NO: 576; SEQ ID NO: 577; SEQ ID NO: 578; SEQ ID NO: 579; SEQ ID NO: 580; SEQ ID NO: 581; SEQ ID NO: 582; SEQ ID NO: 583; SEQ ID NO: 584; SEQ ID NO: 585; SEQ ID NO: 586; SEQ ID NO: 587; SEQ ID NO: 588; SEQ ID NO: 589; SEQ ID NO: 590; SEQ ID NO: 591; SEQ ID NO: 592; SEQ ID NO: 593; SEQ ID NO: 594; SEQ ID NO: 595; SEQ ID NO: 596; SEQ ID NO: 597; SEQ ID NO: 598; SEQ ID NO: 599; SEQ ID NO: 600; SEQ ID NO: 601; SEQ ID NO: 602; SEQ ID NO: 603; SEQ ID NO: 604; SEQ ID NO: 605; SEQ ID NO: 606; SEQ ID NO: 607; SEQ ID NO: 608; SEQ ID NO: 609; SEQ ID NO: 610; SEQ ID NO: 611; SEQ ID NO: 612; SEQ ID NO: 613; SEQ ID NO: 614; SEQ ID NO: 615; SEQ ID NO: 616; SEQ ID NO: 617; SEQ ID NO: 618; SEQ ID NO: 619; SEQ ID NO: 620; SEQ ID NO: 621; SEQ ID NO: 622; SEQ ID NO: 623; SEQ ID NO: 624; SEQ ID NO: 625; SEQ ID NO: 626; SEQ ID NO: 627; SEQ ID NO: 628; SEQ ID NO: 629; SEQ ID NO: 630; SEQ ID NO: 631; SEQ ID NO: 632; SEQ ID NO: 633; SEQ ID NO: 634; SEQ ID NO: 635; SEQ ID NO: 636; SEQ ID NO: 637; SEQ ID NO: 638; SEQ ID NO: 639; SEQ ID NO: 640; SEQ ID NO: 641; SEQ ID NO: 642; SEQ ID NO: 643; SEQ ID NO: 644; SEQ ID NO: 645; SEQ ID NO: 646; SEQ ID NO: 647; SEQ ID NO: 648; SEQ ID NO: 649; SEQ ID NO: 650; SEQ ID NO: 651; SEQ ID NO: 652; SEQ ID NO: 653; SEQ ID NO: 654; SEQ ID NO: 655; SEQ ID NO: 656; SEQ ID NO: 657; SEQ ID NO: 658; SEQ ID NO: 659; SEQ ID NO: 660; SEQ ID NO: 661; SEQ ID NO: 662; SEQ ID NO: 663; SEQ ID NO: 664; SEQ ID NO: 665; SEQ ID NO: 666; SEQ ID NO: 667; SEQ ID NO: 668; SEQ ID NO: 669; SEQ ID NO: 670; SEQ ID NO: 671; SEQ ID NO: 672; SEQ ID NO: 673; SEQ ID NO: 674; SEQ ID NO: 675; SEQ ID NO: 676; SEQ ID NO: 677; SEQ ID NO: 678; SEQ ID NO: 679; SEQ ID NO: 680; SEQ ID NO: 681; SEQ ID NO: 682; SEQ ID NO: 682; SEQ ID NO: 683; SEQ ID NO: 684; SEQ ID NO: 685; SEQ ID NO: 686; SEQ ID NO: 687; SEQ ID NO: 688; SEQ ID NO: 689; SEQ ID NO: 690; SEQ ID NO: 691; SEQ ID NO: 692; SEQ ID NO: 693; SEQ ID NO: 694; SEQ ID NO: 695; SEQ ID NO: 696; SEQ ID NO: 697; SEQ ID NO: 698; SEQ ID NO: 699; SEQ ID NO: 700; SEQ ID NO: 701; SEQ ID NO: 702; SEQ ID NO: 703; SEQ ID NO: 704; SEQ ID NO: 705; SEQ ID NO: 706; SEQ ID NO: 707; SEQ ID NO: 708; SEQ ID NO: 709; SEQ ID NO: 710; SEQ ID NO: 711; SEQ ID NO: 712; SEQ ID NO: 713; SEQ ID NO: 714; SEQ ID NO: 715; SEQ ID NO: 716; SEQ ID NO: 717; SEQ ID NO: 718; SEQ ID NO: 719; SEQ ID NO: 720; SEQ ID NO: 721; SEQ ID NO: 722; SEQ ID NO: 723; SEQ ID NO: 724; SEQ ID NO: 725; SEQ ID NO: 726; SEQ ID NO: 727; SEQ ID NO: 728; SEQ ID NO: 729; SEQ ID NO: 730; SEQ ID NO: 731; SEQ ID NO: 732; SEQ ID NO: 733; SEQ ID NO: 734; SEQ ID NO: 735; SEQ ID NO: 736; SEQ ID NO: 737; SEQ ID NO: 738; SEQ ID NO: 739; SEQ ID NO: 740; SEQ ID NO: 741; SEQ ID NO: 742; SEQ ID NO: 743; SEQ ID NO: 744; SEQ ID NO: 745; SEQ ID NO: 746; SEQ ID NO: 747; SEQ ID NO: 748; SEQ ID NO: 749; SEQ ID NO: 750; SEQ ID NO: 751; SEQ ID NO: 752; SEQ ID NO: 753; SEQ ID NO: 754; SEQ ID NO: 755; SEQ ID NO: 756; SEQ ID NO: 757; SEQ ID NO: 758; SEQ ID NO: 759; SEQ ID NO: 760; SEQ ID NO: 761; SEQ ID NO: 762; SEQ ID NO: 763; SEQ ID NO: 764; SEQ ID NO: 765; SEQ ID NO: 766; SEQ ID NO: 767; SEQ ID NO: 768; SEQ ID NO: 769; SEQ ID NO: 770; SEQ ID NO: 771; SEQ ID NO: 772; SEQ ID NO: 773; SEQ ID NO: 774; SEQ ID NO: 775; SEQ ID NO: 776; SEQ ID NO: 777; SEQ ID NO: 778; SEQ ID NO: 779; SEQ ID NO: 780; SEQ ID NO: 781; SEQ ID NO: 782; SEQ ID NO: 783; SEQ ID NO: 784; SEQ ID NO: 785; SEQ ID NO: 786; SEQ ID NO: 787; SEQ ID NO: 788; SEQ ID NO: 789; SEQ ID NO: 790; SEQ ID NO: 791; SEQ ID NO: 792; SEQ ID NO: 793; SEQ ID NO: 794; SEQ ID NO: 795; SEQ ID NO: 796; SEQ ID NO: 797; SEQ ID NO: 798; SEQ ID NO: 799; SEQ ID NO: 800; SEQ ID NO: 801; SEQ ID NO: 802; SEQ ID NO: 9537; SEQ ID NO: 9538; SEQ ID NO: 9539; SEQ ID NO: 9540; SEQ ID NO: 9541; SEQ ID NO: 9542; SEQ ID NO: 9543; SEQ ID NO: 9544; SEQ ID NO: 9545; SEQ ID NO: 9546; SEQ ID NO: 9547; SEQ ID NO: 9548; SEQ ID NO: 9549; SEQ ID NO: 9550; SEQ ID NO: 9551; SEQ ID NO: 9552; SEQ ID NO: 9553 and SEQ ID NO: 9554.  
     [0124] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in amino acid metabolism and transport encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 683; SEQ ID NO: 684; SEQ ID NO: 685; SEQ ID NO: 686; SEQ ID NO: 687; SEQ ID NO: 688; SEQ ID NO: 689; SEQ ID NO: 690; SEQ ID NO: 691; SEQ ID NO: 692; SEQ ID NO: 693; SEQ ID NO: 694; SEQ ID NO: 695; SEQ ID NO: 696; SEQ ID NO: 697; SEQ ID NO: 698; SEQ ID NO: 699; SEQ ID NO: 700; SEQ ID NO: 701; SEQ ID NO: 702; SEQ ID NO: 703; SEQ ID NO: 704; SEQ ID NO: 705; SEQ ID NO: 706; SEQ ID NO: 707; SEQ ID NO: 708; SEQ ID NO: 709; SEQ ID NO: 710; SEQ ID NO: 711; SEQ ID NO: 712; SEQ ID NO: 713; SEQ ID NO: 714; SEQ ID NO: 715; SEQ ID NO: 716; SEQ ID NO: 717; SEQ ID NO: 718; SEQ ID NO: 719; SEQ ID NO: 720; SEQ ID NO: 9547 and SEQ ID NO: 9548.  
     [0125] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in nucleotide, lipid, or cofactor metabolism and transport encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 721; SEQ ID NO: 722; SEQ ID NO: 723; SEQ ID NO: 724; SEQ ID NO: 725; SEQ ID NO: 726; SEQ ID NO: 727; SEQ ID NO: 728; SEQ ID NO: 729; SEQ ID NO: 730; SEQ ID NO: 731; SEQ ID NO: 732; SEQ ID NO: 733; SEQ ID NO: 734; SEQ ID NO: 735; SEQ ID NO: 736; SEQ ID NO: 737; SEQ ID NO: 738; SEQ ID NO: 739; SEQ ID NO: 740; SEQ ID NO: 741; SEQ ID NO: 742; SEQ ID NO: 743; SEQ ID NO: 744; SEQ ID NO: 745; SEQ ID NO: 746; SEQ ID NO: 747; SEQ ID NO: 748; SEQ ID NO: 749; SEQ ID NO: 750; SEQ ID NO: 751; SEQ ID NO: 752; SEQ ID NO: 753; SEQ ID NO: 754; SEQ ID NO: 755; SEQ ID NO: 756; SEQ ID NO: 757; SEQ ID NO: 758; SEQ ID NO: 759; SEQ ID NO: 760; SEQ ID NO: 761; SEQ ID NO: 762; SEQ ID NO: 763; SEQ ID NO: 764 and SEQ ID NO: 9549.  
     [0126] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in inorganic ion transport encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 765; SEQ ID NO: 766; SEQ ID NO: 767; SEQ ID NO: 768; SEQ ID NO: 769; SEQ ID NO: 770; SEQ ID NO: 771; SEQ ID NO: 772; SEQ ID NO: 773; SEQ ID NO: 774; SEQ ID NO: 775; SEQ ID NO: 776; SEQ ID NO: 777; SEQ ID NO: 778; SEQ ID NO: 779; SEQ ID NO: 780; SEQ ID NO: 781; SEQ ID NO: 782; SEQ ID NO: 783; SEQ ID NO: 784; SEQ ID NO: 785; SEQ ID NO: 786; SEQ ID NO: 787; SEQ ID NO: 788; SEQ ID NO: 789; SEQ ID NO: 790; SEQ ID NO: 791; SEQ ID NO: 792; SEQ ID NO: 793; SEQ ID NO: 794; SEQ ID NO: 795; SEQ ID NO: 796; SEQ ID NO: 797; SEQ ID NO: 798; SEQ ID NO: 799; SEQ ID NO: 9550; SEQ ID NO: 9551; SEQ ID NO: 9552; SEQ ID NO: 9553 and SEQ ID NO: 9554.  
     [0127] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in carbohydrate metabolism and transport encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 800; SEQ ID NO: 801; SEQ ID NO: 802.  
     [0128] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in outer membrane and cell wall formation encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 803; SEQ ID NO: 804; SEQ ID NO: 805; SEQ ID NO: 806; SEQ ID NO: 807; SEQ ID NO: 808; SEQ ID NO: 809; SEQ ID NO: 810; SEQ ID NO: 811; SEQ ID NO: 812; SEQ ID NO: 813; SEQ ID NO: 814; SEQ ID NO: 815; SEQ ID NO: 816; SEQ ID NO: 817; SEQ ID NO: 818; SEQ ID NO: 819; SEQ ID NO: 820; SEQ ID NO: 821; SEQ ID NO: 822; SEQ ID NO: 823; SEQ ID NO: 824; SEQ ID NO: 825; SEQ ID NO: 826; SEQ ID NO: 827; SEQ ID NO: 828; SEQ ID NO: 829; SEQ ID NO: 830; SEQ ID NO: 831; SEQ ID NO: 832; SEQ ID NO: 833; SEQ ID NO: 834; SEQ ID NO: 835; SEQ ID NO: 836; SEQ ID NO: 837; SEQ ID NO: 838; SEQ ID NO: 839; SEQ ID NO: 840; SEQ ID NO: 841; SEQ ID NO: 842; SEQ ID NO: 843; SEQ ID NO: 844; SEQ ID NO: 845; SEQ ID NO: 846; SEQ ID NO: 847; SEQ ID NO: 848; SEQ ID NO: 9555; SEQ ID NO: 9556; SEQ ID NO: 9557; SEQ ID NO: 9558; SEQ ID NO: 9559 and SEQ ID NO: 9560.  
     [0129] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in energy conversion encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 849; SEQ ID NO: 850; SEQ ID NO: 851; SEQ ID NO: 852; SEQ ID NO: 853; SEQ ID NO: 854; SEQ ID NO: 855; SEQ ID NO: 856; SEQ ID NO: 857; SEQ ID NO: 858; SEQ ID NO: 859; SEQ ID NO: 860; SEQ ID NO: 861; SEQ ID NO: 862; SEQ ID NO: 863; SEQ ID NO: 864; SEQ ID NO: 865; SEQ ID NO: 866; SEQ ID NO: 867; SEQ ID NO: 868; SEQ ID NO: 869; SEQ ID NO: 870; SEQ ID NO: 871; SEQ ID NO: 872; SEQ ID NO: 873; SEQ ID NO: 874; SEQ ID NO: 875; SEQ ID NO: 876; SEQ ID NO: 877; SEQ ID NO: 878; SEQ ID NO: 879; SEQ ID NO: 880; SEQ ID NO: 881; SEQ ID NO: 882; SEQ ID NO: 883; SEQ ID NO: 884; SEQ ID NO: 885; SEQ ID NO: 886; SEQ ID NO: 887; SEQ ID NO: 888; SEQ ID NO: 889; SEQ ID NO: 890; SEQ ID NO: 891; SEQ ID NO: 892; SEQ ID NO: 893; SEQ ID NO: 894; SEQ ID NO: 895; SEQ ID NO: 896; SEQ ID NO: 897; SEQ ID NO: 898; SEQ ID NO: 899; SEQ ID NO: 900; SEQ ID NO: 901; SEQ ID NO: 902; SEQ ID NO: 903; SEQ ID NO: 904; SEQ ID NO: 905; SEQ ID NO: 906; SEQ ID NO: 907; SEQ ID NO: 908; SEQ ID NO: 909; SEQ ID NO: 910; SEQ ID NO: 911; SEQ ID NO: 912; SEQ ID NO: 913; SEQ ID NO: 914; SEQ ID NO: 915; SEQ ID NO: 916; SEQ ID NO: 917; SEQ ID NO: 918; SEQ ID NO: 919; SEQ ID NO: 920; SEQ ID NO: 921; SEQ ID NO: 922; SEQ ID NO: 923; SEQ ID NO: 924; SEQ ID NO: 925; SEQ ID NO: 926; SEQ ID NO: 927; SEQ ID NO: 928; SEQ ID NO: 929; SEQ ID NO: 930; SEQ ID NO: 931; SEQ ID NO: 932; SEQ ID NO: 933; SEQ ID NO: 934; SEQ ID NO: 935; SEQ ID NO: 936; SEQ ID NO: 937; SEQ ID NO: 938; SEQ ID NO: 939; SEQ ID NO: 940; SEQ ID NO: 941; SEQ ID NO: 942; SEQ ID NO: 943; SEQ ID NO: 944; SEQ ID NO: 945; SEQ ID NO: 946; SEQ ID NO: 947; SEQ ID NO: 948; SEQ ID NO: 949; SEQ ID NO: 950; SEQ ID NO: 951; SEQ ID NO: 952; SEQ ID NO: 953; SEQ ID NO: 954; SEQ ID NO: 955; SEQ ID NO: 956; SEQ ID NO: 957; SEQ ID NO: 958; SEQ ID NO: 959; SEQ ID NO: 960; SEQ ID NO: 961; SEQ ID NO: 962; SEQ ID NO: 963; SEQ ID NO: 964; SEQ ID NO: 965; SEQ ID NO: 966; SEQ ID NO: 967; SEQ ID NO: 968; SEQ ID NO: 969; SEQ ID NO: 970; SEQ ID NO: 971; SEQ ID NO: 972; SEQ ID NO: 973; SEQ ID NO: 974; SEQ ID NO: 975; SEQ ID NO: 976; SEQ ID NO: 977; SEQ ID NO: 978; SEQ ID NO: 979; SEQ ID NO: 980; SEQ ID NO: 981; SEQ ID NO: 982; SEQ ID NO: 983; SEQ ID NO: 984; SEQ ID NO: 985; SEQ ID NO: 986; SEQ ID NO: 987; SEQ ID NO: 988; SEQ ID NO: 989; SEQ ID NO: 990; SEQ ID NO: 991; SEQ ID NO: 992; SEQ ID NO: 993; SEQ ID NO: 994; SEQ ID NO: 995; SEQ ID NO: 9561; SEQ ID NO: 9562; SEQ ID NO: 9563; SEQ ID NO: 9564 and SEQ ID NO: 9565.  
     [0130] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in secretion and adhesion encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 996; SEQ ID NO: 997; SEQ ID NO: 998; SEQ ID NO: 999; SEQ ID NO: 1000; SEQ ID NO: 1001; SEQ ID NO: 1002; SEQ ID NO: 1003; SEQ ID NO: 1004; SEQ ID NO: 1005; SEQ ID NO: 1006; SEQ ID NO: 1007 and SEQ ID NO: 9566.  
     [0131] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in regulation encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1008; SEQ ID NO: 1009; SEQ ID NO: 1010; SEQ ID NO: 1011; SEQ ID NO: 1012; SEQ ID NO 1013; SEQ ID NO: 1014; SEQ ID NO: 1015; SEQ ID NO: 1016; SEQ ID NO: 1017; SEQ ID NO: 1018; SEQ ID NO: 1019; SEQ ID NO: 1020; SEQ ID NO: 1021; SEQ ID NO: 1022; SEQ ID NO: 1023; SEQ ID NO: 1024; SEQ ID NO: 1025; SEQ ID NO: 1026 and SEQ ID NO: 1027.  
     [0132] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane chaperone polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1028; SEQ ID NO: 1029; SEQ ID NO: 1030; SEQ ID NO: 1031 and SEQ ID NO: 9567.  
     [0133] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in cell division encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1032; SEQ ID NO: 1033; SEQ ID NO: 1034; SEQ ID NO: 1035; SEQ ID NO: 1036; SEQ ID NO: 1037; SEQ ID NO: 1038; SEQ ID NO: 1039; SEQ ID NO: 1040; SEQ ID NO: 1041; SEQ ID NO: 1042; SEQ ID NO: 1043; SEQ ID NO: 1044; SEQ ID NO: 1045; SEQ ID NO: 1046; SEQ ID NO: 1047; SEQ ID NO: 1048; SEQ ID NO: 1049; SEQ ID NO: 1050; SEQ ID NO: 1051; SEQ ID NO: 1052; SEQ ID NO: 9568 and SEQ ID NO: 9569.  
     [0134] In another embodiment, the  H. pylori  cell envelope polypeptide or a fragment thereof is an  H. pylori  inner membrane polypeptide or a fragment thereof involved in motility encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1053 and SEQ ID NO: 1054.  
     [0135] Particularly preferred is an isolated  H. pylori  cytoplasmic polypeptide or a fragment thereof, wherein the polypeptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 6338; SEQ ID NO: 6339; SEQ ID NO: 6340; SEQ ID NO: 6341; SEQ ID NO: 6342; SEQ ID NO: 6343; SEQ ID NO: 6344; SEQ ID NO: 6345; SEQ ID NO: 6346; SEQ ID NO: 6347; SEQ ID NO: 6348; SEQ ID NO: 6349; SEQ ID NO: 6350; SEQ ID NO: 6351; SEQ ID NO: 6352; SEQ ID NO: 6353; SEQ ID NO: 6354; SEQ ID NO: 6355; SEQ ID NO: 6356; SEQ ID NO: 6357; SEQ ID NO: 6358; SEQ ID NO: 6359; SEQ ID NO: 6360; SEQ ID NO: 6361; SEQ ID NO: 6362; SEQ ID NO: 6363; SEQ ID NO: 6364; SEQ ID NO: 6365; SEQ ID NO: 6366; SEQ ID NO: 6367; SEQ ID NO: 6368; SEQ ID NO: 6369; SEQ ID NO: 6370; SEQ ID NO: 6371; SEQ ID NO: 6372; SEQ ID NO: 6373; SEQ ID NO: 6374; SEQ ID NO: 6375; SEQ ID NO: 6376; SEQ ID NO: 6377; SEQ ID NO: 6378; SEQ ID NO: 6379; SEQ ID NO: 6380; SEQ ID NO: 6381; SEQ ID NO: 6382; SEQ ID NO: 6383; SEQ ID NO: 6384; SEQ ID NO: 6385; SEQ ID NO: 6386; SEQ ID NO: 6387; SEQ ID NO: 6388; SEQ ID NO: 6389; SEQ ID NO: 6390; SEQ ID NO: 6391; SEQ ID NO: 6392; SEQ ID NO: 6393; SEQ ID NO: 6394; SEQ ID NO: 6395; SEQ ID NO: 6396; SEQ ID NO: 6397; SEQ ID NO: 6398; SEQ ID NO: 6399; SEQ ID NO: 6400; SEQ ID NO: 6401; SEQ ID NC. 6402; SEQ ID NO: 6403; SEQ ID NO: 6404; SEQ ID NO: 6405; SEQ ID NO: 6406; SEQ ID NO: 6407; SEQ ID NO: 6408; SEQ ID NO: 6409; SEQ ID NO: 6410; SEQ ID NO: 6411; SEQ ID NO: 6412; SEQ ID NO: 6413; SEQ ID NO: 6414; SEQ ID NO: 6415; SEQ ID NO: 6416; SEQ ID NO: 6417; SEQ ID NO: 6418; SEQ ID NO: 6419; SEQ ID NO: 6420; SEQ ID NO: 6421; SEQ ID NO: 6422; SEQ ID NO: 6423; SEQ ID NO: 6424; SEQ ID NO: 6425; SEQ ID NO: 6426; SEQ ID NO: 6427; SEQ ID NO: 6428; SEQ ID NO: 6429; SEQ ID NO: 6430; SEQ ID NO: 6431; SEQ ID NO: 6432; SEQ ID NO: 6433; SEQ ID NO: 6434; SEQ ID NO: 6435; SEQ ID NO: 6436; SEQ ID NO: 6437; SEQ ID NO: 6438; SEQ ID NO: 6439; SEQ ID NO: 6440; SEQ ID NO: 6441; SEQ ID NO: 6442; SEQ ID NO: 6443; SEQ ID NO: 6444; SEQ ID NO: 6445; SEQ ID NO: 6446; SEQ ID NO: 6447; SEQ ID NO: 6448; SEQ ID NO: 6449; SEQ ID NO: 6450; SEQ ID NO: 6451; SEQ ID NO: 6452; SEQ ID NO: 6453; SEQ ID NO: 6454; SEQ ID NO: 6455; SEQ ID NO: 6456; SEQ ID NO: 6457; SEQ ID NO: 6458; SEQ ID NO: 6459; SEQ ID NO: 6460; SEQ ID NO: 6461; SEQ ID NO: 6462; SEQ ID NO: 6463; SEQ ID NO: 6464; SEQ ID NO: 6465; SEQ ID NO: 6466; SEQ ID NO: 6467; SEQ ID NO: 6468; SEQ ID NO: 6469; SEQ ID NO: 6470; SEQ ID NO: 6471; SEQ ID NO: 6472; SEQ ID NO: 6473; SEQ ID NO: 6474; SEQ ID NO: 6475; SEQ ID NO: 6476; SEQ ID NO: 6477; SEQ ID NO: 6478; SEQ ID NO: 6479; SEQ ID NO: 6480; SEQ ID NO: 6481; SEQ ID NO: 6482; SEQ ID NO: 6483; SEQ ID NO: 6484; SEQ ID NO: 6485; SEQ ID NO: 6486; SEQ ID NO: 6487; SEQ ID NO: 6488; SEQ ID NO: 6489; SEQ ID NO: 6490; SEQ ID NO: 6491; SEQ ID NO: 6492; SEQ ID NO: 6493; SEQ ID NO: 6494; SEQ ID NO: 6495; SEQ ID NO: 6496; SEQ ID NO: 6497; SEQ ID NO: 6498; SEQ ID NO: 6499; SEQ ID NO: 6500; SEQ ID NO: 6501; SEQ ID NO: 6502; SEQ ID NO: 6503; SEQ ID NO: 6504; SEQ ID NO: 6505; SEQ ID NO: 6506; SEQ ID NO: 6507; SEQ ID NO: 6508; SEQ ID NO: 6509; SEQ ID NO: 6510; SEQ ID NO: 6511; SEQ ID NO: 6512; SEQ ID NO: 6513; SEQ ID NO: 6514; SEQ ID NO: 6515; SEQ ID NO: 6516; SEQ ID NO: 6517; SEQ ID NO: 6518; SEQ ID NO: 6519; SEQ ID NO: 6520; SEQ ID NO: 6521; SEQ ID NO: 6522; SEQ ID NO: 6523; SEQ ID NO: 6524; SEQ ID NO: 6525; SEQ ID NO: 6526; SEQ ID NO: 6527; SEQ ID NO: 6528; SEQ ID NO: 6529; SEQ ID NO: 6530; SEQ ID NO: 6531; SEQ ID NO: 6532; SEQ ID NO: 6533; SEQ ID NO: 6534; SEQ ID NO: 6535; SEQ ID NO: 6536; SEQ ID NO: 6537; SEQ ID NO: 6538; SEQ ID NO: 6539; SEQ ID NO: 6540; SEQ ID NO: 6541; SEQ ID NO: 6542; SEQ ID NO: 6543; SEQ ID NO: 6544; SEQ ID NO: 6545; SEQ ID NO: 6546; SEQ ID NO: 6547; SEQ ID NO: 6548; SEQ ID NO: 6549; SEQ ID NO: 6550; SEQ ID NO: 6551; SEQ ID NO: 6552; SEQ ID NO: 6553; SEQ ID NO: 6554; SEQ ID NO: 6555; SEQ ID NO: 6556; SEQ ID NO: 6557; SEQ ID NO: 6558; SEQ ID NO: 6559; SEQ ID NO: 6560; SEQ ID NO: 6561; SEQ ID NO: 6562; SEQ ID NO: 6563; SEQ ID NO: 6564; SEQ ID NO: 6565; SEQ ID NO: 6566; SEQ ID NO: 6567; SEQ ID NO: 6568; SEQ ID NO: 6569; SEQ ID NO: 6570; SEQ ID NO: 6571; SEQ ID NO: 6572; SEQ ID NO: 6573; SEQ ID NO: 6574; SEQ ID NO: 6575; SEQ ID NO: 6576; SEQ ID NO: 6577; SEQ ID NO: 6578; SEQ ID NO: 6579; SEQ ID NO: 6580; SEQ ID NO: 6581; SEQ ID NO: 6582; SEQ ID NO: 6583; SEQ ID NO: 6584; SEQ ID NO: 6585; SEQ ID NO: 6586; SEQ ID NO: 6587; SEQ ID NO: 6588; SEQ ID NO: 6589; SEQ ID NO: 6590; SEQ ID NO: 6591; SEQ ID NO: 6592; SEQ ID NO: 6593; SEQ ID NO: 6594; SEQ ID NO: 6595; SEQ ID NO: 6596; SEQ ID NO: 6597; SEQ ID NO: 6598; SEQ ID NO: 6599; SEQ ID NO: 6600; SEQ ID NO: 6601; SEQ ID NO: 6602; SEQ ID NO: 6603; SEQ ID NO: 6604; SEQ ID NO: 6605; SEQ ID NO: 6606; SEQ ID NO: 6607; SEQ ID NO: 6608; SEQ ID NO: 6609; SEQ ID NO: 6610; SEQ ID NO: 6611; SEQ ID NO: 6612; SEQ ID NO: 6613; SEQ ID NO: 6614; SEQ ID NO: 6615; SEQ ID NO: 6616; SEQ ID NO: 6617; SEQ ID NO: 6618; SEQ ID NO: 6619; SEQ ID NO: 6620; SEQ ID NO: 6621; SEQ ID NO: 6622; SEQ ID NO: 6623; SEQ ID NO: 6624; SEQ ID NO: 6625; SEQ ID NO: 6626; SEQ ID NO: 6627; SEQ ID NO: 6628; SEQ ID NO: 6629; SEQ ID NO: 6630; SEQ ID NO: 6631; SEQ ID NO: 6632; SEQ ID NO: 6633; SEQ ID NO: 6634; SEQ ID NO: 6635; SEQ ID NO: 6636; SEQ ID NO: 6637; SEQ ID NO: 6638; SEQ ID NO: 6639; SEQ ID NO: 6640; SEQ ID NO: 6641; SEQ ID NO: 6642; SEQ ID NO: 6643; SEQ ID NO: 6644; SEQ ID NO: 6645; SEQ ID NO: 6646; SEQ ID NO: 6647; SEQ ID NO: 6648; SEQ ID NO: 6649; SEQ ID NO: 6650; SEQ ID NO: 6651; SEQ ID NO: 6652; SEQ ID NO: 6653; SEQ ID NO: 6654; SEQ ID NO: 6655; SEQ ID NO: 6656; SEQ ID NO: 6657; SEQ ID NO: 6658; SEQ ID NO: 6659; SEQ ID NO: 6660; SEQ ID NO: 6661; SEQ ID NO: 6662; SEQ ID NO: 6663; SEQ ID NO: 6664; SEQ ID NO: 6665; SEQ ID NO: 6666; SEQ ID NO: 6667; SEQ ID NO: 6668; SEQ ID NO: 6669; SEQ ID NO: 6670; SEQ ID NO: 6671; SEQ ID NO: 6672; SEQ ID NO: 6673; SEQ ID NO: 6674; SEQ ID NO: 6675; SEQ ID NO: 6676; SEQ ID NO: 6677; SEQ ID NO: 6678; SEQ ID NO: 6679; SEQ ID NO: 6680; SEQ ID NO: 6681; SEQ ID NO: 6682; SEQ ID NO: 6683; SEQ ID NO: 6684; SEQ ID NO: 6685; SEQ ID NO: 6686; SEQ ID NO: 6687; SEQ ID NO: 6688; SEQ ID NO: 6689; SEQ ID NO: 6690; SEQ ID NO: 6691; SEQ ID NO: 6692; SEQ ID NO: 6693; SEQ ID NO: 6694; SEQ ID NO: 6695; SEQ ID NO: 6696; SEQ ID NO: 6697; SEQ ID NO: 6698; SEQ ID NO: 6699; SEQ ID NO: 6700; SEQ ID NO: 6701; SEQ ID NO: 6702; SEQ ID NO: 6703; SEQ ID NO: 6704; SEQ ID NO: 6705; SEQ ID NO: 6706; SEQ ID NO: 6707; SEQ ID NO: 6708; SEQ ID NO: 6709; SEQ ID NO: 6710; SEQ ID NO: 6711; SEQ ID NO: 6712; SEQ ID NO: 6713; SEQ ID NO: 6714; SEQ ID NO: 6715; SEQ ID NO: 6716; SEQ ID NO: 6717; SEQ ID NO: 6718; SEQ ID NO: 6719; SEQ ID NO: 6720; SEQ ID NO: 6721; SEQ ID NO: 6722; SEQ ID NO: 6723; SEQ ID NO: 6724; SEQ ID NO: 6725; SEQ ID NO: 6726; SEQ ID NO: 6727; SEQ ID NO: 6728; SEQ ID NO: 6729; SEQ ID NO: 6730; SEQ ID NO: 6731; SEQ ID NO: 6732; SEQ ID NO: 6733; SEQ ID NO: 6734; SEQ ID NO: 6735; SEQ ID NO: 6736; SEQ ID NO: 6737; SEQ ID NO: 6738; SEQ ID NO: 6739; SEQ ID NO: 6740; SEQ ID NO: 6741; SEQ ID NO: 6742; SEQ ID NO: 6743; SEQ ID NO: 6744; SEQ ID NO: 6745; SEQ ID NO: 6746; SEQ ID NO: 6747; SEQ ID NO: 6748; SEQ ID NO: 6749; SEQ ID NO: 6750; SEQ ID NO: 6751; SEQ ID NO: 6752; SEQ ID NO: 6753; SEQ ID NO: 6754; SEQ ID NO: 6755; SEQ ID NO: 6756; SEQ ID NO: 6757; SEQ ID NO: 6758; SEQ ID NO: 6759; SEQ ID NO: 6760; SEQ ID NO: 6761; SEQ ID NO: 6762; SEQ ID NO: 6763; SEQ ID NO: 6764; SEQ ID NO: 6765; SEQ ID NO: 6766; SEQ ID NO: 6767; SEQ ID NO: 6768; SEQ ID NO: 6769; SEQ ID NO: 6770; SEQ ID NO: 6771; SEQ ID NO: 6772; SEQ ID NO: 6773; SEQ ID NO: 6774; SEQ ID NO: 6775; SEQ ID NO: 6776; SEQ ID NO: 6777; SEQ ID NO: 6778; SEQ ID NO: 6779; SEQ ID NO: 6780; SEQ ID NO: 6781; SEQ ID NO: 6782; SEQ ID NO: 6783; SEQ ID NO: 6784; SEQ ID NO: 6785; SEQ ID NO: 6786; SEQ ID NO: 6787; SEQ ID NO: 6788; SEQ ID NO: 6789; SEQ ID NO: 6790; SEQ ID NO: 6791; SEQ ID NO: 6792; SEQ ID NO: 6793; SEQ ID NO: 6794; SEQ ID NO: 6795; SEQ ID NO: 6796; SEQ ID NO: 6797; SEQ ID NO: 6798; SEQ ID NO: 6799; SEQ ID NO: 6800; SEQ ID NO: 6801; SEQ ID NO: 6802; SEQ ID NO: 6803; SEQ ID NO: 6804; SEQ ID NO: 6805; SEQ ID NO: 6806; SEQ ID NO: 6807; SEQ ID NO: 6808; SEQ ID NO: 6809; SEQ ID NO: 6810; SEQ ID NO: 6811; SEQ ID NO: 6812; SEQ ID NO: 6813; SEQ ID NO: 6814; SEQ ID NO: 6815; SEQ ID NO: 6816; SEQ ID NO: 6817; SEQ ID NO: 6818; SEQ ID NO: 6819; SEQ ID NO: 6820; SEQ ID NO: 6821; SEQ ID NO: 6822; SEQ ID NO: 6823; SEQ ID NO: 6824; SEQ ID NO: 6825; SEQ ID NO: 6826; SEQ ID NO: 6827; SEQ ID NO: 6828; SEQ ID NO: 6829; SEQ ID NO: 6830; SEQ ID NO: 6831; SEQ ID NO: 6832; SEQ ID NO: 6833; SEQ ID NO: 6834; SEQ ID NO: 6835; SEQ ID NO: 6836; SEQ ID NO: 6837; SEQ ID NO: 6838; SEQ ID NO: 6839; SEQ ID NO: 6840; SEQ ID NO: 6841; SEQ ID NO: 6842; SEQ ID NO: 6843; SEQ ID NO: 6844; SEQ ID NO: 6845; SEQ ID NO: 6846; SEQ ID NO: 6847; SEQ ID NO: 6848; SEQ ID NO: 6849; SEQ ID NO: 6850; SEQ ID NO: 6851; SEQ ID NO: 6852; SEQ ID NO: 6853; SEQ ID NO: 6854; SEQ ID NO: 6855; SEQ ID NO: 6856; SEQ ID NO: 6857; SEQ ID NO: 6858; SEQ ID NO: 6859; SEQ ID NO: 6860; SEQ ID NO: 6861; SEQ ID NO: 6862; SEQ ID NO: 6863; SEQ ID NO: 6864; SEQ ID NO: 6865; SEQ ID NO: 6866; SEQ ID NO: 6867; SEQ ID NO: 6868; SEQ ID NO: 6869; SEQ ID NO: 6870; SEQ ID NO: 6871; SEQ ID NO: 6872; SEQ ID NO: 6873; SEQ ID NO: 6874; SEQ ID NO: 6875; SEQ ID NO: 6876; SEQ ID NO: 6877; SEQ ID NO: 6878; SEQ ID NO: 6879; SEQ ID NO: 6880; SEQ ID NO: 6881; SEQ ID NO: 6882; SEQ ID NO: 6883; SEQ ID NO: 6884; SEQ ID NO: 6885; SEQ ID NO: 6886; SEQ ID NO: 6887; SEQ ID NO: 6888; SEQ ID NO: 6889; SEQ ID NO: 6890; SEQ ID NO: 6891; SEQ ID NO: 6892; SEQ ID NO: 6893; SEQ ID NO: 6894; SEQ ID NO: 6895; SEQ ID NO: 6896; SEQ ID NO: 6897; SEQ ID NO: 6898; SEQ ID NO: 6899; SEQ ID NO: 6900; SEQ ID NO: 6901; SEQ ID NO: 6902; SEQ ID NO: 6903; SEQ ID NO: 6904; SEQ ID NO: 6905; SEQ ID NO: 6906; SEQ ID NO: 6907; SEQ ID NO: 6908; SEQ ID NO: 6909; SEQ ID NO: 6910; SEQ ID NO: 6911; SEQ ID NO: 6912; SEQ ID NO: 6913; SEQ ID NO: 6914; SEQ ID NO: 6915; SEQ ID NO: 6916; SEQ ID NO: 6917; SEQ ID NO: 6918; SEQ ID NO: 6919; SEQ ID NO: 6920; SEQ ID NO: 6921; SEQ ID NO: 6922; SEQ ID NO: 6923; SEQ ID NO: 6924; SEQ ID NO: 6925; SEQ ID NO: 6926; SEQ ID NO: 6927; SEQ ID NO: 6928; SEQ ID NO: 6929; SEQ ID NO: 6930; SEQ ID NO: 6931; SEQ ID NO: 6932; SEQ ID NO: 6933; SEQ ID NO: 6934; SEQ ID NO: 6935; SEQ ID NO: 6936; SEQ ID NO: 6937; SEQ ID NO: 6938; SEQ ID NO: 6939; SEQ ID NO: 6940; SEQ ID NO: 6941; SEQ ID NO: 6942; SEQ ID NO: 6943; SEQ ID NO: 6944; SEQ ID NO: 6945; SEQ ID NO: 6946; SEQ ID NO: 6947; SEQ ID NO: 6948; SEQ ID NO: 6949; SEQ ID NO: 6950; SEQ ID NO: 6951; SEQ ID NO: 6952; SEQ ID NO: 6953; SEQ ID NO: 6954; SEQ ID NO: 6955; SEQ ID NO: 6956; SEQ ID NO: 6957; SEQ ID NO: 6958; SEQ ID NO: 6959; SEQ ID NO: 6960; SEQ ID NO: 6961; SEQ ID NO: 6962; SEQ ID NO: 6963; SEQ ID NO: 6964; SEQ ID NO: 6965; SEQ ID NO: 6966; SEQ ID NO: 6967; SEQ ID NO: 6968; SEQ ID NO: 6969; SEQ ID NO: 6970; SEQ ID NO: 6971; SEQ ID NO: 6972; SEQ ID NO: 6973; SEQ ID NO: 6974; SEQ ID NO: 6975; SEQ ID NO: 6976; SEQ ID NO: 6977; SEQ ID NO: 6978; SEQ ID NO: 6979; SEQ ID NO: 6980; SEQ ID NO: 6981; SEQ ID NO: 6982; SEQ ID NO: 6983; SEQ ID NO: 6984; SEQ ID NO: 6985; SEQ ID NO: 6986; SEQ ID NO: 6987; SEQ ID NO: 6988; SEQ ID NO: 6989; SEQ ID NO: 6990; SEQ ID NO: 6991; SEQ ID NO: 6992; SEQ ID NO: 6993; SEQ ID NO: 6994; SEQ ID NO: 6995; SEQ ID NO: 6996; SEQ ID NO: 6997; SEQ ID NO: 6998; SEQ ID NO: 6999; SEQ ID NO: 7000; SEQ ID NO: 7001; SEQ ID NO: 7002; SEQ ID NO: 7003; SEQ ID NO: 7004; SEQ ID NO: 7005; SEQ ID NO: 7006; SEQ ID NO: 7007; SEQ ID NO: 7008; SEQ ID NO: 7009; SEQ ID NO: 7010; SEQ ID NO: 7011; SEQ ID NO: 7012; SEQ ID NO: 7013; SEQ ID NO: 7014; SEQ ID NO: 7015; SEQ ID NO: 7016; SEQ ID NO: 7017; SEQ ID NO: 7018; SEQ ID NO: 7019; SEQ ID NO: 7020; SEQ ID NO: 7021; SEQ ID NO: 7022; SEQ ID NO: 7023; SEQ ID NO: 7024; SEQ ID NO: 7025; SEQ ID NO: 7026; SEQ ID NO: 7027; SEQ ID NO: 7028; SEQ ID NO: 7029; SEQ ID NO: 7030; SEQ ID NO: 7031; SEQ ID NO: 7032; SEQ ID NO: 7033; SEQ ID NO: 7034; SEQ ID NO: 7035; SEQ ID NO: 7036; SEQ ID NO: 7037; SEQ ID NO: 7038; SEQ ID NO: 7039; SEQ ID NO: 7040; SEQ ID NO: 7041; SEQ ID NO: 7042; SEQ ID NO: 7043; SEQ ID NO: 7044; SEQ ID NO: 7045; SEQ ID NO: 7046; SEQ ID NO: 7047; SEQ ID NO: 7048; SEQ ID NO: 7049; SEQ ID NO: 7050; SEQ ID NO: 7051; SEQ ID NO: 7052; SEQ ID NO: 7053; SEQ ID NO: 7054; SEQ ID NO: 7055; SEQ ID NO-7056; SEQ ID NO: 7057; SEQ ID NO: 7058; SEQ ID NO: 7059; SEQ ID NO: 7060; SEQ ID NO: 7061; SEQ ID NO: 7062; SEQ ID NO: 7063; SEQ ID NO: 7064; SEQ ID NO: 7065; SEQ ID NO: 7066; SEQ ID NO: 7067; SEQ ID NO: 7068; SEQ ID NO: 7069; SEQ ID NO: 7070; SEQ ID NO: 7071; SEQ ID NO: 7072; SEQ ID NO: 7073; SEQ ID NO: 7074; SEQ ID NO: 7075; SEQ ID NO: 7076; SEQ ID NO: 7077; SEQ ID NO: 7078; SEQ ID NO: 7079; SEQ ID NO: 7080; SEQ ID NO: 7081; SEQ ID NO: 7082; SEQ ID NO: 7083; SEQ ID NO: 7084; SEQ ID NO: 7085; SEQ ID NO: 7086; SEQ ID NO: 7087; SEQ ID NO: 7088; SEQ ID NO: 7089; SEQ ID NO: 7090; SEQ ID NO: 7091; SEQ ID NO: 7092; SEQ ID NO: 7093; SEQ ID NO: 7094; SEQ ID NO: 7095; SEQ ID NO: 7096; SEQ ID NO: 7097; SEQ ID NO: 7098; SEQ ID NO: 7099; SEQ ID NO: 7100; SEQ ID NO: 7101; SEQ ID NO: 7102; SEQ ID NO: 7103; SEQ ID NO: 7104; SEQ ID NO: 7105; SEQ ID NO: 7106; SEQ ID NO: 7107; SEQ ID NO: 7108; SEQ ID NO: 7109; SEQ ID NO: 7110; SEQ ID NO: 7111; SEQ ID NO: 7112; SEQ ID NO: 7113; SEQ ID NO: 7114; SEQ ID NO: 7115; SEQ ID NO: 7116; SEQ ID NO: 7117; SEQ ID NO: 7118; SEQ ID NO: 7119; SEQ ID NO: 7120; SEQ ID NO: 7121; SEQ ID NO: 7122; SEQ ID NO: 7123; SEQ ID NO: 7124; SEQ ID NO: 7125; SEQ ID NO: 7126; SEQ ID NO: 7127; SEQ ID NO: 7128; SEQ ID NO: 7129; SEQ ID NO: 7130; SEQ ID NO: 7131; SEQ ID NO: 7132; SEQ ID NO: 7133; SEQ ID NO: 7134; SEQ ID NO: 7135; SEQ ID NO: 7136; SEQ ID NO: 7137; SEQ ID NO: 7138; SEQ ID NO: 7139; SEQ ID NO: 7140; SEQ ID NO: 7141; SEQ ID NO: 7142; SEQ ID NO: 7143; SEQ ID NO: 7144; SEQ ID NO: 7145; SEQ ID NO: 7146; SEQ ID NO: 7147; SEQ ID NO: 7148; SEQ ID NO: 7149; SEQ ID NO: 7150; SEQ ID NO: 7151; SEQ ID NO: 7152; SEQ ID NO: 7153; SEQ ID NO: 7154; SEQ ID NO: 7155; SEQ ID NO: 7156; SEQ ID NO: 7157; SEQ ID NO: 7158; SEQ ID NO: 7159; SEQ ID NO: 7160; SEQ ID NO: 7161; SEQ ID NO: 7162; SEQ ID NO: 7163; SEQ ID NO: 7164; SEQ ID NO: 7165; SEQ ID NO: 7166; SEQ ID NO: 7167; SEQ ID NO: 7168; SEQ ID NO: 7169; SEQ ID NO: 7170; SEQ ID NO: 7171; SEQ ID NO: 7172; SEQ ID NO: 7173; SEQ ID NO: 7174; SEQ ID NO: 7175; SEQ ID NO: 7176; SEQ ID NO: 7177; SEQ ID NO: 7178; SEQ ID NO: 7179; SEQ ID NO: 7180; SEQ ID NO: 7181; SEQ ID NO: 7182; SEQ ID NO: 7183; SEQ ID NO: 7184; SEQ ID NO: 7185; SEQ ID NO: 7186; SEQ ID NO: 7187; SEQ ID NO: 7188; SEQ ID NO: 7189; SEQ ID NO: 7190; SEQ ID NO: 7191; SEQ ID NO: 7192; SEQ ID NO: 7193; SEQ ID NO: 7194; SEQ ID NO: 7195; SEQ ID NO: 7196; SEQ ID NO: 7197; SEQ ID NO: 7198; SEQ ID NO: 7199; SEQ ID NO: 7200; SEQ ID NO: 7201; SEQ ID NO: 7202; SEQ ID NO: 7203; SEQ ID NO: 7204; SEQ ID NO: 7205; SEQ ID NO: 7206; SEQ ID NO: 7207; SEQ ID NO: 7208; SEQ ID NO: 7209; SEQ ID NO: 7210; SEQ ID NO: 7211; SEQ ID NO: 7212; SEQ ID NO: 7213; SEQ ID NO: 7214; SEQ ID NO: 7215; SEQ ID NO: 7216; SEQ ID NO: 7217; SEQ ID NO: 7218; SEQ ID NO: 7219; SEQ ID NO: 7220; SEQ ID NO: 7221; SEQ ID NO: 7222; SEQ ID NO: 7223; SEQ ID NO: 7224; SEQ ID NO: 7225; SEQ ID NO: 7226; SEQ ID NO: 7227; SEQ ID NO: 7228; SEQ ID NO: 7229; SEQ ID NO: 7230; SEQ ID NO: 7231; SEQ ID NO: 7232; SEQ ID NO: 7233; SEQ ID NO: 7234; SEQ ID NO: 7235; SEQ ID NO: 7236; SEQ ID NO: 7237; SEQ ID NO: 7238; SEQ ID NO: 7239; SEQ ID NO: 7240; SEQ ID NO: 7241; SEQ ID NO: 7242; SEQ ID NO: 7243; SEQ ID NO: 7244; SEQ ID NO: 7245; SEQ ID NO: 7246; SEQ ID NO: 7247; SEQ ID NO: 7248; SEQ ID NO: 7249; SEQ ID NO: 7250; SEQ ID NO: 7251; SEQ ID NO: 7252; SEQ ID NO: 7253; SEQ ID NO: 7254; SEQ ID NO: 7255; SEQ ID NO: 7256; SEQ ID NO: 7257; SEQ ID NO: 7258; SEQ ID NO: 7259; SEQ ID NO: 7260; SEQ ID NO: 7261; SEQ ID NO: 7262; SEQ ID NO: 7263; SEQ ID NO: 7264; SEQ ID NO: 7265; SEQ ID NO: 7266; SEQ ID NO: 7267; SEQ ID NO: 7268; SEQ ID NO: 7269; SEQ ID NO: 7270; SEQ ID NO: 7271; SEQ ID NO: 7272; SEQ ID NO: 7273; SEQ ID NO: 7274; SEQ ID NO: 7275; SEQ ID NO: 7276; SEQ ID NO: 7277; SEQ ID NO: 7278; SEQ ID NO: 7279; SEQ ID NO: 7280; SEQ ID NO: 7281; SEQ ID NO: 7282; SEQ ID NO: 7283; SEQ ID NO: 7284; SEQ ID NO: 7285; SEQ ID NO: 7286; SEQ ID NO: 7287; SEQ ID NO: 7288; SEQ ID NO: 7289; SEQ ID NO: 7290; SEQ ID NO: 7291; SEQ ID NO: 7292; SEQ ID NO:7293; SEQ ID NO: 7294; SEQ ID NO: 7295; SEQ ID NO: 7296; SEQ ID NO: 7297; SEQ ID NO: 7298; SEQ ID NO: 7299; SEQ ID NO: 7300; SEQ ID NO: 7301; SEQ ID NO: 7302; SEQ ID NO: 7303; SEQ ID NO: 7304; SEQ ID NO: 7305; SEQ ID NO: 7306; SEQ ID NO: 7307; SEQ ID NO: 7308; SEQ ID NO: 7309; SEQ ID NO: 7310; SEQ ID NO: 7311; SEQ ID NO: 7312; SEQ ID NO: 7313; SEQ ID NO: 7314; SEQ ID NO: 7315; SEQ ID NO: 7316; SEQ ID NO: 7317; SEQ ID NO: 7318; SEQ ID NO: 7319; SEQ ID NO: 7320; SEQ ID NO: 7321; SEQ ID NO: 7322; SEQ ID NO: 7323; SEQ ID NO: 7324; SEQ ID NO: 7325; SEQ ID NO: 7326; SEQ ID NO: 7327; SEQ ID NO: 7328; SEQ ID NO: 7329; SEQ ID NO: 7330; SEQ ID NO: 7331; SEQ ID NO: 7332; SEQ ID NO: 7333; SEQ ID NO: 7334; SEQ ID NO: 7335; SEQ ID NO: 7336; SEQ ID NO: 7337; SEQ ID NO: 7338; SEQ ID NO: 7339; SEQ ID NO: 7340; SEQ ID NO: 7341; SEQ ID NO: 7342; SEQ ID NO: 7343; SEQ ID NO: 7344; SEQ ID NO: 7345; SEQ ID NO: 7346; SEQ ID NO: 7347; SEQ ID NO: 7348; SEQ ID NO: 7349; SEQ ID NO: 7350; SEQ ID NO: 7351; SEQ ID NO: 7352; SEQ ID NO: 7353; SEQ ID NO: 7354; SEQ ID NO: 7355; SEQ ID NO: 7356; SEQ ID NO: 7357; SEQ ID NO: 7358; SEQ ID NO: 7359; SEQ ID NO: 7360; SEQ ID NO: 7361; SEQ ID NO: 7362; SEQ ID NO: 7363; SEQ ID NO: 7364; SEQ ID NO: 7365; SEQ ID NO: 7366; SEQ ID NO: 7367; SEQ ID NO: 7368; SEQ ID NO: 7369; SEQ ID NO: 7370; SEQ ID NO: 7371; SEQ ID NO: 7372; SEQ ID NO: 7373; SEQ ID NO: 7374; SEQ ID NO: 7375; SEQ ID NO: 7376; SEQ ID NO: 7377; SEQ ID NO: 7378; SEQ ID NO: 7379; SEQ ID NO: 7380; SEQ ID NO: 7381; SEQ ID NO: 7382; SEQ ID NO: 7383; SEQ ID NO: 7384; SEQ ID NO: 7385; SEQ ID NO: 7386; SEQ ID NO: 7387; SEQ ID NO: 7388; SEQ ID NO: 7389; SEQ ID NO: 7390; SEQ ID NO: 7391; SEQ ID NO: 7392; SEQ ID NO: 7393; SEQ ID NO: 7394; SEQ ID NO: 7395; SEQ ID NO: 7396; SEQ ID NO: 7397; SEQ ID NO: 7398; SEQ ID NO: 7399; SEQ ID NO: 7400; SEQ ID NO: 7401; SEQ ID NO: 7402; SEQ ID NO: 7403; SEQ ID NO: 7404; SEQ ID NO: 7405; SEQ ID NO: 7406; SEQ ID NO: 7407; SEQ ID NO: 7408; SEQ ID NO: 7409; SEQ ID NO: 7410; SEQ ID NO: 7411; SEQ ID NO: 7412; SEQ ID NO: 7413; SEQ ID NO: 7414; SEQ ID NO: 7415; SEQ ID NO: 7416; SEQ ID NO: 7417; SEQ ID NO: 7418; SEQ ID NO: 7419; SEQ ID NO: 7420; SEQ ID NO: 7421; SEQ ID NO: 7422; SEQ ID NO: 7423; SEQ ID NO: 7424; SEQ ID NO: 7425; SEQ ID NO: 7426; SEQ ID NO: 7427; SEQ ID NO: 7428; SEQ ID NO: 7429; SEQ ID NO: 7430; SEQ ID NO: 7431; SEQ ID NO: 7432; SEQ ID NO: 7433; SEQ ID NO: 7434; SEQ ID NO: 7435; SEQ ID NO: 7436; SEQ ID NO: 7437; SEQ ID NO: 7438; SEQ ID NO: 7439; SEQ ID NO: 7440; SEQ ID NO: 7441; SEQ ID NO: 7442; SEQ ID NO: 7443; SEQ ID NO: 7444; SEQ ID NO: 7445; SEQ ID NO: 7446; SEQ ID NO: 7447; SEQ ID NO: 7448; SEQ ID NO: 7449; SEQ ID NO: 7450; SEQ ID NO: 7451; SEQ ID NO: 7452; SEQ ID NO: 7453; SEQ ID NO: 7454; SEQ ID NO: 7455; SEQ ID NO: 7456; SEQ ID NO: 7457; SEQ ID NO: 7458; SEQ ID NO: 7459; SEQ ID NO: 7460; SEQ ID NO: 7461; SEQ ID NO: 7462; SEQ ID NO: 7463; SEQ ID NO: 7464; SEQ ID NO: 7465; SEQ ID NO: 7466; SEQ ID NO: 7467; SEQ ID NO: 7468; SEQ ID NO: 7469; SEQ ID NO: 7470; SEQ ID NO: 7471; SEQ ID NO: 7472; SEQ ID NO: 7473; SEQ ID NO: 7474; SEQ ID NO: 7475; SEQ ID NO: 7476; SEQ ID NO: 7477; SEQ ID NO: 7478; SEQ ID NO: 7479; SEQ ID NO: 7480; SEQ ID NO: 7481; SEQ ID NO: 7482; SEQ ID NO: 7483; SEQ ID NO: 7484; SEQ ID NO: 7485; SEQ ID NO: 7486; SEQ ID NO: 7487; SEQ ID NO: 7488; SEQ ID NO: 7489; SEQ ID NO: 7490; SEQ ID NO: 7491; SEQ ID NO: 7492; SEQ ID NO: 7493; SEQ ID NO: 7494; SEQ ID NO: 7495; SEQ ID NO: 7496; SEQ ID NO: 7497; SEQ ID NO: 7498; SEQ ID NO: 7499; SEQ ID NO: 7500; SEQ ID NO: 7501; SEQ ID NO: 7502; SEQ ID NO: 7503; SEQ ID NO: 7504; SEQ ID NO: 7505; SEQ ID NO: 7506; SEQ ID NO: 7507; SEQ ID NO: 7508; SEQ ID NO: 7509; SEQ ID NO: 7510; SEQ ID NO: 7511; SEQ ID NO: 7512; SEQ ID NO: 7513; SEQ ID NO: 7514; SEQ ID NO: 7515; SEQ ID NO: 7516; SEQ ID NO: 7517; SEQ ID NO: 7518; SEQ ID NO: 7519; SEQ ID NO: 7520; SEQ ID NO: 7521; SEQ ID NO: 7522; SEQ ID NO: 7523; SEQ ID NO: 7524; SEQ ID NO: 7525; SEQ ID NO: 7526; SEQ ID NO: 7527; SEQ ID NO: 7528; SEQ ID NO: 7529; SEQ ID NO: 7530; SEQ ID NO: 7531; SEQ ID NO: 7532; SEQ ID NO: 7533; SEQ ID NO: 7534; SEQ ID NO: 7535; SEQ ID NO: 7536; SEQ ID NO: 7537; SEQ ID NO: 7538; SEQ ID NO: 7539; SEQ ID NO: 7540; SEQ ID NO: 7541; SEQ ID NO: 7542; SEQ ID NO: 7543; SEQ ID NO: 7544; SEQ ID NO: 7545; SEQ ID NO: 7546; SEQ ID NO: 7547; SEQ ID NO: 7548; SEQ ID NO: 7549; SEQ ID NO: 7550; SEQ ID NO: 7551; SEQ ID NO: 7552; SEQ ID NO: 7553; SEQ ID NO: 7554; SEQ ID NO: 7555; SEQ ID NO: 7556; SEQ ID NO: 7557; SEQ ID NO: 7558; SEQ ID NO: 7559; SEQ ID NO: 7560; SEQ ID NO: 7561; SEQ ID NO: 7562; SEQ ID NO: 7563; SEQ ID NO: 7564; SEQ ID NO: 7565; SEQ ID NO: 7566; SEQ ID NO: 7567; SEQ ID NO: 7568; SEQ ID NO: 7569; SEQ ID NO: 7570; SEQ ID NO: 7571; SEQ ID NO: 7572; SEQ ID NO: 7573; SEQ ID NO: 7574; SEQ ID NO: 7575; SEQ ID NO: 7576; SEQ ID NO: 7577; SEQ ID NO: 7578; SEQ ID NO: 7579; SEQ ID NO: 7580; SEQ ID NO: 7581; SEQ ID NO: 7582; SEQ ID NO: 7583; SEQ ID NO: 7584; SEQ ID NO: 7585; SEQ ID NO: 7586; SEQ ID NO: 7587; SEQ ID NO: 7588; SEQ ID NO: 7589; SEQ ID NO: 7590; SEQ ID NO: 7591; SEQ ID NO: 7592; SEQ ID NO: 7593; SEQ ID NO: 7594; SEQ ID NO: 7595; SEQ ID NO: 7596; SEQ ID NO: 7597; SEQ ID NO: 7598; SEQ ID NO: 7599; SEQ ID NO: 7600; SEQ ID NO: 7601; SEQ ID NO: 7602; SEQ ID NO: 7603; SEQ ID NO: 7604; SEQ ID NO: 7605; SEQ ID NO: 7606; SEQ ID NO: 7607; SEQ ID NO: 7608; SEQ ID NO: 7609; SEQ ID NO: 7610; SEQ ID NO: 7611; SEQ ID NO: 7612; SEQ ID NO: 7613; SEQ ID NO: 7614; SEQ ID NO: 7615; SEQ ID NO: 7616; SEQ ID NO: 7617; SEQ ID NO: 7618; SEQ ID NO: 7619; SEQ ID NO: 7620; SEQ ID NO: 7621; SEQ ID NO: 7622; SEQ ID NO: 7623; SEQ ID NO: 7624; SEQ ID NO: 7625; SEQ ID NO: 7626; SEQ ID NO: 7627; SEQ ID NO: 7628; SEQ ID NO: 7629; SEQ ID NO: 7630; SEQ ID NO: 7631; SEQ ID NO: 7632; SEQ ID NO: 7633; SEQ ID NO: 7634; SEQ ID NO: 7635; SEQ ID NO: 7636; SEQ ID NO: 7637; SEQ ID NO: 7638; SEQ ID NO: 7639; SEQ ID NO: 7640; SEQ ID NO: 7641; SEQ ID NO: 7642; SEQ ID NO: 7643; SEQ ID NO: 7644; SEQ ID NO: 7645; SEQ ID NO: 7646; SEQ ID NO: 7647; SEQ ID NO: 7648; SEQ ID NO: 7649; SEQ ID NO: 7650; SEQ ID NO: 7651; SEQ ID NO: 7652; SEQ ID NO: 7653; SEQ ID NO: 7654; SEQ ID NO: 7655; SEQ ID NO: 7656; SEQ ID NO: 7657; SEQ ID NO: 7658; SEQ ID NO: 7659; SEQ ID NO: 7660; SEQ ID NO: 7661; SEQ ID NO: 7662; SEQ ID NO: 7663; SEQ ID NO: 7664; SEQ ID NO: 7665; SEQ ID NO: 7666; SEQ ID NO: 7667; SEQ ID NO: 7668; SEQ ID NO: 7669; SEQ ID NO: 7670; SEQ ID NO: 7671; SEQ ID NO: 7672; SEQ ID NO: 7673; SEQ ID NO: 7674; SEQ ID NO: 7675; SEQ ID NO: 7676; SEQ ID NO: 7677; SEQ ID NO: 7678; SEQ ID NO: 7679; SEQ ID NO: 7680; SEQ ID NO: 7681; SEQ ID NO: 7682; SEQ ID NO: 7683; SEQ ID NO: 7684; SEQ ID NO: 7685; SEQ ID NO: 7686; SEQ ID NO: 7687; SEQ ID NO: 7688; SEQ ID NO: 7689; SEQ ID NO: 7690; SEQ ID NO: 7691; SEQ ID NO: 7692; SEQ ID NO: 7693; SEQ ID NO: 7694; SEQ ID NO: 7695; SEQ ID NO: 7696; SEQ ID NO: 7697; SEQ ID NO: 7698; SEQ ID NO: 7699; SEQ ID NO: 7700; SEQ ID NO: 7701; SEQ ID NO: 7702; SEQ ID NO: 7703; SEQ ID NO: 7704; SEQ ID NO: 7705; SEQ ID NO: 7706; SEQ ID NO: 7707; SEQ ID NO: 7708; SEQ ID NO: 7709; SEQ ID NO: 7710; SEQ ID NO: 7711; SEQ ID NO: 7712; SEQ ID NO: 7713; SEQ ID NO: 7714; SEQ ID NO: 7715; SEQ ID NO: 7716; SEQ ID NO: 7717; SEQ ID NO: 7718; SEQ ID NO: 7719; SEQ ID NO: 7720; SEQ ID NO: 7721; SEQ ID NO: 7722; SEQ ID NO: 7723; SEQ ID NO: 7724; SEQ ID NO: 7725; SEQ ID NO: 7726; SEQ ID NO: 7727; SEQ ID NO: 7728; SEQ ID NO: 7729; SEQ ID NO: 7730; SEQ ID NO: 7731; SEQ ID NO: 7732; SEQ ID NO: 7733; SEQ ID NO: 7734; SEQ ID NO: 7735; SEQ ID NO: 7736; SEQ ID NO: 7737; SEQ ID NO: 7738; SEQ ID NO: 7739; SEQ ID NO: 7740; SEQ ID NO: 7741; SEQ ID NO: 7742; SEQ ID NO: 7743; SEQ ID NO: 7744; SEQ ID NO: 7745; SEQ ID NO: 7746; SEQ ID NO: 7747; SEQ ID NO: 7748; SEQ ID NO: 7749; SEQ ID NO: 7750; SEQ ID NO: 7751; SEQ ID NO: 7752; SEQ ID NO: 7753; SEQ ID NO: 7754; SEQ ID NO: 7755; SEQ ID NO: 7756; SEQ ID NO: 7757; SEQ ID NO: 7758; SEQ ID NO: 7759; SEQ ID NO: 7760; SEQ ID NO: 7761; SEQ ID NO: 7762; SEQ ID NO: 7763; SEQ ID NO: 7764; SEQ ID NO: 7765; SEQ ID NO: 7766; SEQ ID NO: 7767; SEQ ID NO: 7768; SEQ ID NO: 7769; SEQ ID NO: 7770; SEQ ID NO: 7771; SEQ ID NO: 7772; SEQ ID NO: 7773; SEQ ID NO: 7774; SEQ ID NO: 7775; SEQ ID NO: 7776; SEQ ID NO: 7777; SEQ ID NO: 7778; SEQ ID NO: 7779; SEQ ID NO: 7780; SEQ ID NO: 7781; SEQ ID NO: 7782; SEQ ID NO: 7783; SEQ ID NO: 7784; SEQ ID NO: 7785; SEQ ID NO: 7786; SEQ ID NO: 7787; SEQ ID NO: 7788; SEQ ID NO: 7789; SEQ ID NO: 7790; SEQ ID NO: 7791; SEQ ID NO: 7792; SEQ ID NO: 7793; SEQ ID NO: 7794; SEQ ID NO: 7795; SEQ ID NO: 7796; SEQ ID NO: 7797; SEQ ID NO: 7798; SEQ ID NO: 7799; SEQ ID NO: 7800; SEQ ID NO: 7801; SEQ ID NO: 7802; SEQ ID NO: 7803; SEQ ID NO: 7804; SEQ ID NO: 7805; SEQ ID NO: 7806; SEQ ID NO: 7807; SEQ ID NO: 7808; SEQ ID NO: 7809; SEQ ID NO: 7810; SEQ ID NO: 7811; SEQ ID NO: 7812; SEQ ID NO: 7813; SEQ ID NO: 7814; SEQ ID NO: 7815; SEQ ID NO: 7816; SEQ ID NO: 7817; SEQ ID NO: 7818; SEQ ID NO: 7819; SEQ ID NO: 7820; SEQ ID NO: 7821; SEQ ID NO: 7822; SEQ ID NO: 7823; SEQ ID NO: 7824; SEQ ID NO: 7825; SEQ ID NO: 7826; SEQ ID NO: 7827; SEQ ID NO: 7828; SEQ ID NO: 7829; SEQ ID NO: 7830; SEQ ID NO: 7831; SEQ ID NO: 7832; SEQ ID NO: 7833; SEQ ID NO: 7834; SEQ ID NO: 7835; SEQ ID NO: 7836; SEQ ID NO: 7837; SEQ ID NO: 7838; SEQ ID NO: 7839; SEQ ID NO: 7840; SEQ ID NO: 7841; SEQ ID NO: 7842; SEQ ID NO: 7843; SEQ ID NO: 7844; SEQ ID NO: 7845; SEQ ID NO: 7846; SEQ ID NO: 7847; SEQ ID NO: 7848; SEQ ID NO: 7849; SEQ ID NO: 7850; SEQ ID NO: 7851; SEQ ID NO: 7852; SEQ ID NO: 7853; SEQ ID NO: 7854; SEQ ID NO: 7855; SEQ ID NO: 7856; SEQ ID NO: 7857; SEQ ID NO: 7858; SEQ ID NO: 7859; SEQ ID NO: 7860; SEQ ID NO: 7861; SEQ ID NO: 7862; SEQ ID NO: 7863; SEQ ID NO: 7864; SEQ ID NO: 7865; SEQ ID NO: 7866; SEQ ID NO: 7867; SEQ ID NO: 7868; SEQ ID NO: 7869; SEQ ID NO: 7870; SEQ ID NO: 7871; SEQ ID NO: 7872; SEQ ID NO: 7873; SEQ ID NO: 7874; SEQ ID NO: 7875; SEQ ID NO: 7876; SEQ ID NO: 7877; SEQ ID NO: 7878; SEQ ID NO: 7879; SEQ ID NO: 7880; SEQ ID NO: 7881; SEQ ID NO: 7882; SEQ ID NO. 7883; SEQ ID NO: 7884; SEQ ID NO: 7885; SEQ ID NO: 7886; SEQ ID NO: 788; SEQ ID NO: 7888; SEQ ID NO: 7889; SEQ ID NO: 7890; SEQ ID NO: 7891; SEQ ID NO: 7892; SEQ ID NO: 7893; SEQ ID NO: 7894; SEQ ID NO: 7895; SEQ ID NO: 7896; SEQ ID NO: 7897; SEQ ID NO: 7898; SEQ ID NO: 7899; SEQ ID NO: 7900; SEQ ID NO: 7901; SEQ ID NO: 7902; SEQ ID NO: 7903; SEQ ID NO: 7904; SEQ ID NO: 7905; SEQ ID NO: 7906; SEQ ID NO: 7907; SEQ ID NO: 7908; SEQ ID NO: 7909; SEQ ID NO: 7910; SEQ ID NO: 7911; SEQ ID NO: 7912; SEQ ID NO: 7913; SEQ ID NO: 7914; SEQ ID NO: 7915; SEQ ID NO: 7916; SEQ ID NO: 7917; SEQ ID NO: 7918; SEQ ID NO: 7919; SEQ ID NO: 7920; SEQ ID NO: 7921; SEQ ID NO: 7922; SEQ ID NO: 7923; SEQ ID NO: 7924; SEQ ID NO: 7925; SEQ ID NO: 7926; SEQ ID NO: 7927; SEQ ID NO: 7928; SEQ ID NO: 7929; SEQ ID NO: 7930; SEQ ID NO: 7931; SEQ ID NO: 7932; SEQ ID NO: 7933; SEQ ID NO: 7934; SEQ ID NO: 7935; SEQ ID NO: 7936; SEQ ID NO: 7937; SEQ ID NO: 7938; SEQ ID NO: 7939; SEQ ID NO: 7940; SEQ ID NO: 7941; SEQ ID NO: 7942; SEQ ID NO: 7943; SEQ ID NO: 7944; SEQ ID NO: 7945; SEQ ID NO: 7946; SEQ ID NO: 7947; SEQ ID NO: 7948; SEQ ID NO: 7949; SEQ ID NO: 7950; SEQ ID NO: 7951; SEQ ID NO: 7952; SEQ ID NO: 7953; SEQ ID NO: 7954; SEQ ID NO: 7955; SEQ ID NO: 7956; SEQ ID NO: 7957; SEQ ID NO: 7958; SEQ ID NO: 7959; SEQ ID NO: 7960; SEQ ID NO: 7961; SEQ ID NO: 7962; SEQ ID NO: 7963; SEQ ID NO: 7964; SEQ ID NO: 7965; SEQ ID NO: 7966; SEQ ID NO: 7967; SEQ ID NO: 7968; SEQ ID NO: 7969; SEQ ID NO: 7970; SEQ ID NO: 7971; SEQ ID NO: 7972; SEQ ID NO: 7973; SEQ ID NO: 7974; SEQ ID NO: 7975; SEQ ID NO: 7976; SEQ ID NO: 7977; SEQ ID NO: 7978; SEQ ID NO: 7979; SEQ ID NO: 7980; SEQ ID NO: 7981; SEQ ID NO: 7982; SEQ ID NO: 7983; SEQ ID NO: 7984; SEQ ID NO: 7985; SEQ ID NO: 7986; SEQ ID NO: 7987; SEQ ID NO: 7988; SEQ ID NO: 7989; SEQ ID NO: 7990; SEQ ID NO: 7991; SEQ ID NO: 7992; SEQ ID NO: 7993; SEQ ID NO: 7994; SEQ ID NO: 7995; SEQ ID NO: 7996; SEQ ID NO: 7997; SEQ ID NO: 7998; SEQ ID NO: 7999; SEQ ID NO: 8000; SEQ ID NO: 8001; SEQ ID NO: 8002; SEQ ID NO: 8003; SEQ ID NO: 8004; SEQ ID NO: 8005; SEQ ID NO: 8006; SEQ ID NO: 8007; SEQ ID NO: 8008; SEQ ID NO: 8009; SEQ ID NO: 8010; SEQ ID NO: 8011; SEQ ID NO: 8012; SEQ ID NO: 8013; SEQ ID NO: 8014; SEQ ID NO: 8015; SEQ ID NO: 8016; SEQ ID NO: 8017; SEQ ID NO: 8018; SEQ ID NO: 8019; SEQ ID NO: 8020; SEQ ID NO: 8021; SEQ ID NO: 8022; SEQ ID NO: 8023; SEQ ID NO: 8024; SEQ ID NO: 8025; SEQ ID NO: 8026; SEQ ID NO: 8027; SEQ ID NO: 8028; SEQ ID NO: 8029; SEQ ID NO: 8030; SEQ ID NO: 8031; SEQ ID NO: 8032; SEQ ID NO: 8033; SEQ ID NO: 8034; SEQ ID NO: 8035; SEQ ID NO: 8036; SEQ ID NO: 8037; SEQ ID NO: 8038; SEQ ID NO: 8039; SEQ ID NO: 8040; SEQ ID NO: 8041; SEQ ID NO: 8042; SEQ ID NO: 8043; SEQ ID NO: 8044; SEQ ID NO: 8045; SEQ ID NO: 8046; SEQ ID NO: 8047; SEQ ID NO: 8048; SEQ ID NO: 8049; SEQ ID NO: 8050; SEQ ID NO: 8051; SEQ ID NO: 8052; SEQ ID NO: 8053; SEQ ID NO: 8054; SEQ ID NO: 8055; SEQ ID NO: 8056; SEQ ID NO: 8057; SEQ ID NO: 8058; SEQ ID NO: 8059; SEQ ID NO: 8060; SEQ ID NO: 8061; SEQ ID NO: 8062; SEQ ID NO: 8063; SEQ ID NO: 8064; SEQ ID NO: 8065; SEQ ID NO: 8066; SEQ ID NO: 8067; SEQ ID NO: 8068; SEQ ID NO: 8069; SEQ ID NO: 8070; SEQ ID NO: 8071; SEQ ID NO: 8072; SEQ ID NO: 8073; SEQ ID NO: 8074; SEQ ID NO: 8075; SEQ ID NO: 8076; SEQ ID NO: 8077; SEQ ID NO: 8078; SEQ ID NO: 8079; SEQ ID NO: 8080; SEQ ID NO: 8081; SEQ ID NO: 8082; SEQ ID NO: 8083; SEQ ID NO: 8084; SEQ ID NO: 8085; SEQ ID NO: 8086; SEQ ID NO: 8087; SEQ ID NO: 8088; SEQ ID NO: 8089; SEQ ID NO: 8090; SEQ ID NO: 8091; SEQ ID NO: 8092; SEQ ID NO: 8093; SEQ ID NO: 8094; SEQ ID NO: 8095; SEQ ID NO: 8096; SEQ ID NO: 8097; SEQ ID NO: 8098; SEQ ID NO: 8099; SEQ ID NO: 8100; SEQ ID NO: 8101; SEQ ID NO: 8102; SEQ ID NO: 8103; SEQ ID NO: 8104; SEQ ID NO: 8105; SEQ ID NO: 8106; SEQ ID NO: 8107; SEQ ID NO: 8108; SEQ ID NO: 8109; SEQ ID NO: 8110; SEQ ID NO: 8111; SEQ ID NO: 8112; SEQ ID NO: 8113; SEQ ID NO: 8114; SEQ ID NO: 8115; SEQ ID NO: 8116; SEQ ID NO:8117; SEQ ID NO: 8118; SEQ ID NO: 8119; SEQ ID NO: 8120; SEQ ID NO: 8121; SEQ ID NO: 8122; SEQ ID NO: 8123; SEQ ID NO: 8124; SEQ ID NO: 8125; SEQ ID NO: 8126; SEQ ID NO: 8127; SEQ ID NO: 8128; SEQ ID NO: 8129; SEQ ID NO: 8130; SEQ ID NO: 8131; SEQ ID NO: 8132; SEQ ID NO: 8133; SEQ ID NO: 8134; SEQ ID NO: 8135; SEQ ID NO: 8136; SEQ ID NO: 8137; SEQ ID NO: 8138; SEQ ID NO: 8139; SEQ ID NO: 8140; SEQ ID NO: 8141; SEQ ID NO: 8142; SEQ ID NO: 8143; SEQ ID NO: 8144; SEQ ID NO: 8145; SEQ ID NO: 8146; SEQ ID NO: 8147; SEQ ID NO: 8148; SEQ ID NO: 8149; SEQ ID NO: 8150; SEQ ID NO: 8151; SEQ ID NO: 8152; SEQ ID NO: 8153; SEQ ID NO: 8154; SEQ ID NO: 8155; SEQ ID NO: 8156; SEQ ID NO: 8157; SEQ ID NO: 8158; SEQ ID NO: 8159; SEQ ID NO: 8160; SEQ ID NO: 8161; SEQ ID NO: 8162; SEQ ID NO: 8163; SEQ ID NO: 8164; SEQ ID NO: 8165; SEQ ID NO: 8166; SEQ ID NO: 8167; SEQ ID NO: 8168; SEQ ID NO: 8169; SEQ ID NO: 8170; SEQ ID NO: 8171; SEQ ID NO: 8172; SEQ ID NO: 8173; SEQ ID NO: 8174; SEQ ID NO: 8175; SEQ ID NO: 8176; SEQ ID NO: 8177; SEQ ID NO: 8178; SEQ ID NO: 8179; SEQ ID NO: 8180; SEQ ID NO: 8181; SEQ ID NO: 8182; SEQ ID NO: 8183; SEQ ID NO: 8184; SEQ ID NO: 8185; SEQ ID NO: 8186; SEQ ID NO: 8187; SEQ ID NO: 8188; SEQ ID NO: 8189; SEQ ID NO: 8190; SEQ ID NO: 8191; SEQ ID NO: 8192; SEQ ID NO: 8193; SEQ ID NO: 8194; SEQ ID NO: 8195; SEQ ID NO: 8196; SEQ ID NO: 8197; SEQ ID NO: 8198; SEQ ID NO: 8199; SEQ ID NO: 8200; SEQ ID NO: 8201; SEQ ID NO: 8202; SEQ ID NO: 8203; SEQ ID NO: 8204; SEQ ID NO: 8205; SEQ ID NO: 8206; SEQ ID NO: 8207; SEQ ID NO: 8208; SEQ ID NO: 8209; SEQ ID NO: 8210; SEQ ID NO: 8211; SEQ ID NO: 8212; SEQ ID NO: 8213; SEQ ID NO: 8214; SEQ ID NO: 8215; SEQ ID NO: 8216; SEQ ID NO: 8217; SEQ ID NO: 8218; SEQ ID NO: 8219; SEQ ID NO: 8220; SEQ ID NO: 8221; SEQ ID NO: 8222; SEQ ID NO: 8223; SEQ ID NO: 8224; SEQ ID NO: 8225; SEQ ID NO: 8226; SEQ ID NO: 8227; SEQ ID NO: 8228; SEQ ID NO: 8229; SEQ ID NO: 8230; SEQ ID NO: 8231; SEQ ID NO: 8232; SEQ ID NO: 8233; SEQ ID NO: 8234; SEQ ID NO: 8235; SEQ ID NO: 8236; SEQ ID NO: 8237; SEQ ID NO: 8238; SEQ ID NO: 8239; SEQ ID NO: 8240; SEQ ID NO: 8241; SEQ ID NO: 8242; SEQ ID NO: 8243; SEQ ID NO: 8244; SEQ ID NO: 8245; SEQ ID NO: 8246; SEQ ID NO: 8247; SEQ ID NO: 8248; SEQ ID NO: 8249; SEQ ID NO: 8250; SEQ ID NO: 8251; SEQ ID NO: 8252; SEQ ID NO: 8253; SEQ ID NO: 8254; SEQ ID NO: 8255; SEQ ID NO: 8256; SEQ ID NO: 8257; SEQ ID NO: 8258; SEQ ID NO: 8259; SEQ ID NO: 8260; SEQ ID NO: 8261; SEQ ID NO: 8262; SEQ ID NO: 8263; SEQ ID NO: 8264; SEQ ID NO: 8265; SEQ ID NO: 8266; SEQ ID NO: 8267; SEQ ID NO: 8268; SEQ ID NO: 8269; SEQ ID NO: 8270; SEQ ID NO: 8271; SEQ ID NO: 8272; SEQ ID NO: 8273; SEQ ID NO: 8274; SEQ ID NO: 8275; SEQ ID NO: 8276; SEQ ID NO: 8277; SEQ ID NO: 8278; SEQ ID NO: 8279; SEQ ID NO: 8280; SEQ ID NO: 8281; SEQ ID NO: 8282; SEQ ID NO: 8283; SEQ ID NO: 8284; SEQ ID NO: 8285; SEQ ID NO: 8286; SEQ ID NO: 8287; SEQ ID NO: 8288; SEQ ID NO: 8289; SEQ ID NO: 8290; SEQ ID NO: 8291; SEQ ID NO: 8292; SEQ ID NO: 8293; SEQ ID NO: 8294; SEQ ID NO: 8295; SEQ ID NO: 8296; SEQ ID NO: 8297; SEQ ID NO: 8298; SEQ ID NO: 8299; SEQ ID NO: 8300; SEQ ID NO: 8301; SEQ ID NO: 8302; SEQ ID NO: 8303; SEQ ID NO: 8304; SEQ ID NO: 8305; SEQ ID NO: 8306; SEQ ID NO: 8307; SEQ ID NO: 8308; SEQ ID NO: 8309; SEQ ID NO: 8310; SEQ ID NO: 8311; SEQ ID NO: 8312; SEQ ID NO: 8313; SEQ ID NO: 8314; SEQ ID NO: 8315; SEQ ID NO: 8316; SEQ ID NO: 8317; SEQ ID NO: 8318; SEQ ID NO: 8319; SEQ ID NO: 8320; SEQ ID NO: 8321; SEQ ID NO: 8322; SEQ ID NO: 8323; SEQ ID NO: 8324; SEQ ID NO: 8325; SEQ ID NO: 8326; SEQ ID NO: 8327; SEQ ID NO: 8328; SEQ ID NO: 8329; SEQ ID NO: 8330; SEQ ID NO: 8331; SEQ ID NO: 8332; SEQ ID NO: 8333; SEQ ID NO: 8334; SEQ ID NO: 8335; SEQ ID NO: 8336; SEQ ID NO: 8337; SEQ ID NO: 8338; SEQ ID NO: 8339; SEQ ID NO: 8340; SEQ ID NO: 8341; SEQ ID NO: 8342; SEQ ID NO: 8343; SEQ ID NO: 8344; SEQ ID NO: 8345; SEQ ID NO: 8346; SEQ ID NO: 8347; SEQ ID NO: 8348; SEQ ID NO: 8349; SEQ ID NO: 8350; SEQ ID NO: 8351; SEQ ID NO: 8352; SEQ ID NO: 8353; SEQ ID NO: 8354; SEQ ID NO: 8355; SEQ ID NO: 8356; SEQ ID NO: 8357; SEQ ID NO: 8358; SEQ ID NO: 8359; SEQ ID NO: 8360; SEQ ID NO: 8361; SEQ ID NO: 8362; SEQ ID NO: 8363; SEQ ID NO: 8364; SEQ ID NO: 8365; SEQ ID NO: 8366; SEQ ID NO: 8367; SEQ ID NO: 8368; SEQ ID NO: 8369; SEQ ID NO: 8370; SEQ ID NO: 8371; SEQ ID NO: 8372; SEQ ID NO: 8373; SEQ ID NO: 8374; SEQ ID NO: 8375; SEQ ID NO: 8376; SEQ ID NO: 8377; SEQ ID NO: 8378; SEQ ID NO: 8379; SEQ ID NO: 8380; SEQ ID NO: 8381; SEQ ID NO: 8382; SEQ ID NO: 8383; SEQ ID NO: 8384; SEQ ID NO: 8385; SEQ ID NO: 8386; SEQ ID NO: 8387; SEQ ID NO: 8388; SEQ ID NO: 8389; SEQ ID NO: 8390; SEQ ID NO: 8391; SEQ ID NO: 8392; SEQ ID NO: 8393; SEQ ID NO: 8394; SEQ ID NO: 8395; SEQ ID NO: 8396; SEQ ID NO: 8397; SEQ ID NO: 8398; SEQ ID NO: 8399; SEQ ID NO: 8400; SEQ ID NO: 8401; SEQ ID NO: 8402; SEQ ID NO: 8403; SEQ ID NO: 8404; SEQ ID NO: 8405; SEQ ID NO: 8406; SEQ ID NO: 8407; SEQ ID NO: 8408; SEQ ID NO: 8409; SEQ ID NO: 8410; SEQ ID NO: 8411; SEQ ID NO: 8412; SEQ ID NO: 8413; SEQ ID NO: 8414; SEQ ID NO: 8415; SEQ ID NO: 8416; SEQ ID NO: 8417; SEQ ID NO: 8418; SEQ ID NO: 8419; SEQ ID NO: 8420; SEQ ID NO: 8421; SEQ ID NO: 8422; SEQ ID NO: 8423; SEQ ID NO: 8424; SEQ ID NO: 8425; SEQ ID NO: 8426; SEQ ID NO: 8427; SEQ ID NO: 8428; SEQ ID NO: 8429; SEQ ID NO: 8430; SEQ ID NO: 8431; SEQ ID NO: 8432; SEQ ID NO: 8433; SEQ ID NO: 8434; SEQ ID NO: 8435; SEQ ID NO: 8436; SEQ ID NO: 8437; SEQ ID NO: 8438; SEQ ID NO: 8439; SEQ ID NO: 8440; SEQ ID NO: 8441; SEQ ID NO: 8442; SEQ ID NO: 8443; SEQ ID NO: 8444; SEQ ID NO: 8445; SEQ ID NO: 8446; SEQ ID NO: 8447; SEQ ID NO: 8448; SEQ ID NO: 8449; SEQ ID NO: 8450; SEQ ID NO: 8451; SEQ ID NO: 8452; SEQ ID NO: 8453; SEQ ID NO: 8454; SEQ ID NO: 8455; SEQ ID NO: 8456; SEQ ID NO: 8457; SEQ ID NO: 8458; SEQ ID NO: 8459; SEQ ID NO: 8460; SEQ ID NO: 8461; SEQ ID NO: 8462; SEQ ID NO: 8463; SEQ ID NO: 8464; SEQ ID NO: 8465; SEQ ID NO: 8466; SEQ ID NO: 8467; SEQ ID NO: 8468; SEQ ID NO: 8469; SEQ ID NO: 8470; SEQ ID NO: 8471; SEQ ID NO: 8472; SEQ ID NO: 8473; SEQ ID NO: 8474; SEQ ID NO: 8475; SEQ ID NO: 8476; SEQ ID NO: 8477; SEQ ID NO: 8478; SEQ ID NO: 8479; SEQ ID NO: 8480; SEQ ID NO: 8481; SEQ ID NO: 8482; SEQ ID NO: 8483; SEQ ID NO: 8484; SEQ ID NO: 8485; SEQ ID NO: 8486; SEQ ID NO: 8487; SEQ ID NO: 8488; SEQ ID NO: 8489; SEQ ID NO: 8490; SEQ ID NO: 8491; SEQ ID NO: 8492; SEQ ID NO: 8493; SEQ ID NO: 8494; SEQ ID NO: 8495; SEQ ID NO: 8496; SEQ ID NO: 8497; SEQ ID NO: 8498; SEQ ID NO: 8499; SEQ ID NO: 8500; SEQ ID NO: 8501; SEQ ID NO: 8502; SEQ ID NO: 8503; SEQ ID NO: 8504; SEQ ID NO: 8505; SEQ ID NO: 8506; SEQ ID NO: 8507; SEQ ID NO: 8508; SEQ ID NO: 8509; SEQ ID NO: 8510; SEQ ID NO: 8511; SEQ ID NO: 8512; SEQ ID NO: 8513; SEQ ID NO: 8514; SEQ ID NO: 8515; SEQ ID NO: 8516; SEQ ID NO: 8517; SEQ ID NO: 8518; SEQ ID NO: 8519; SEQ ID NO: 8520; SEQ ID NO: 8521; SEQ ID NO: 8522; SEQ ID NO: 8523; SEQ ID NO: 8524; SEQ ID NO: 8525; SEQ ID NO: 8526; SEQ ID NO: 8527; SEQ ID NO: 8528; SEQ ID NO: 8529; SEQ ID NO: 8530; SEQ ID NO: 8531; SEQ ID NO: 8532; SEQ ID NO: 8533; SEQ ID NO: 8534; SEQ ID NO: 8535; SEQ ID NO: 8536; SEQ ID NO: 8537; SEQ ID NO: 8538; SEQ ID NO: 8539; SEQ ID NO: 8540; SEQ ID NO: 8541; SEQ ID NO: 8542; SEQ ID NO: 8543; SEQ ID NO: 8544; SEQ ID NO: 8545; SEQ ID NO: 8546; SEQ ID NO: 8547; SEQ ID NO: 8548; SEQ ID NO: 8549; SEQ ID NO: 8550; SEQ ID NO: 8551; SEQ ID NO: 8552; SEQ ID NO: 8553; SEQ ID NO: 8554; SEQ ID NO: 8555; SEQ ID NO: 8556; SEQ ID NO: 8557; SEQ ID NO: 8558; SEQ ID NO: 8559; SEQ ID NO: 8560; SEQ ID NO: 8561; SEQ ID NO: 8562; SEQ ID NO: 8563; SEQ ID NO: 8564; SEQ ID NO: 8565; SEQ ID NO: 8566; SEQ ID NO: 8567; SEQ ID NO: 8568; SEQ ID NO: 8569; SEQ ID NO: 8570; SEQ ID NO: 8571; SEQ ID NO: 8572; SEQ ID NO: 8573; SEQ ID NO: 8574; SEQ ID NO: 8575; SEQ ID NO: 8576; SEQ ID NO: 8577; SEQ ID NO: 8578; SEQ ID NO: 8579; SEQ ID NO: 8580; SEQ ID NO: 8581; SEQ ID NO: 8582; SEQ ID NO: 8583; SEQ ID NO: 8584; SEQ ID NO: 8585; SEQ ID NO: 8586; SEQ ID NO: 8587; SEQ ID NO: 8588; SEQ ID NO: 8589; SEQ ID NO: 8590; SEQ ID NO: 8591; SEQ ID NO: 8592; SEQ ID NO: 8593; SEQ ID NO: 8594; SEQ ID NO: 8595; SEQ ID NO: 8596; SEQ ID NO: 8597; SEQ ID NO: 8598; SEQ ID NO: 8599; SEQ ID NO: 8600; SEQ ID NO: 8601; SEQ ID NO: 8602; SEQ ID NO: 8603; SEQ ID NO: 8604; SEQ ID NO: 8605; SEQ ID NO: 8606; SEQ ID NO: 8607; SEQ ID NO: 8608; SEQ ID NO: 8609; SEQ ID NO: 8610; SEQ ID NO: 8611; SEQ ID NO: 8612; SEQ ID NO: 8613; SEQ ID NO: 8614; SEQ ID NO: 8615; SEQ ID NO: 8616; SEQ ID NO: 8617; SEQ ID NO: 8618; SEQ ID NO: 8619; SEQ ID NO: 8620; SEQ ID NO: 8621; SEQ ID NO: 8622; SEQ ID NO: 8623; SEQ ID NO: 8624; SEQ ID NO: 8625; SEQ ID NO: 8626; SEQ ID NO: 8627; SEQ ID NO: 8628; SEQ ID NO: 8629; SEQ ID NO: 8630; SEQ ID NO: 8631; SEQ ID NO: 8632; SEQ ID NO: 8633; SEQ ID NO: 8634; SEQ ID NO: 8635; SEQ ID NO: 8636; SEQ ID NO: 8637; SEQ ID NO: 8638; SEQ ID NO: 8639; SEQ ID NO: 8640; SEQ ID NO: 8641; SEQ ID NO: 8642; SEQ ID NO: 8643; SEQ ID NO: 8644; SEQ ID NO: 8645; SEQ ID NO: 8646; SEQ ID NO: 8647; SEQ ID NO: 8648; SEQ ID NO: 8649; SEQ ID NO: 8650; SEQ ID NO: 8651; SEQ ID NO: 8652; SEQ ID NO: 8653; SEQ ID NO: 8654; SEQ ID NO: 8655; SEQ ID NO: 8656; SEQ ID NO: 8657; SEQ ID NO: 8658; SEQ ID NO: 8659; SEQ ID NO: 8660; SEQ ID NO: 8661; SEQ ID NO: 8662; SEQ ID NO: 8663; SEQ ID NO: 8664; SEQ ID NO: 8665; SEQ ID NO: 8666; SEQ ID NO: 8667; SEQ ID NO: 8668; SEQ ID NO: 8669; SEQ ID NO: 8670; SEQ ID NO: 8671; SEQ ID NO: 8672; SEQ ID NO: 8673; SEQ ID NO: 8674; SEQ ID NO: 8675; SEQ ID NO: 8676; SEQ ID NO: 8677; SEQ ID NO: 8678; SEQ ID NO: 8679; SEQ ID NO: 8680; SEQ ID NO: 8681; SEQ ID NO: 8682; SEQ ID NO: 8683; SEQ ID NO: 8684; SEQ ID NO: 8685; SEQ ID NO: 8686; SEQ ID NO: 8687; SEQ ID NO: 8688; SEQ ID NO: 8689; SEQ ID NO: 8690; SEQ ID NO: 8691; SEQ ID NO: 8692; SEQ ID NO: 8693; SEQ ID NO: 8694; SEQ ID NO: 8695; SEQ ID NO: 8696; SEQ ID NO: 8697; SEQ ID NO: 8698; SEQ ID NO: 8699; SEQ ID NO: 8700; SEQ ID NO: 8701; SEQ ID NO: 8702; SEQ ID NO: 8703; SEQ ID NO: 8704; SEQ ID NO: 8705; SEQ ID NO: 8706; SEQ ID NO: 8707; SEQ ID NO: 8708; SEQ ID NO: 8709; SEQ ID NO: 8710; SEQ ID NO: 8711; SEQ ID NO: 8712; SEQ ID NO: 8713; SEQ ID NO: 8714; SEQ ID NO: 8715; SEQ ID NO: 8716; SEQ ID NO: 8717; SEQ ID NO: 8718; SEQ ID NO: 8719; SEQ ID NO: 8720; SEQ ID NO: 8721; SEQ ID NO: 8722; SEQ ID NO: 8723; SEQ ID NO: 8724; SEQ ID NO: 8725; SEQ ID NO: 8726; SEQ ID NO: 8727; SEQ ID NO: 8728; SEQ ID NO: 8729; SEQ ID NO: 8730; SEQ ID NO: 8731; SEQ ID NO: 8732; SEQ ID NO: 8733; SEQ ID NO: 8734; SEQ ID NO: 8735; SEQ ID NO: 8736; SEQ ID NO: 8737; SEQ ID NO: 8738; SEQ ID NO: 8739; SEQ ID NO: 8740; SEQ ID NO: 8741; SEQ ID NO: 8742; SEQ ID NO: 8743; SEQ ID NO: 8744; SEQ ID NO: 8745; SEQ ID NO: 8746; SEQ ID NO: 8747; SEQ ID NO: 8748; SEQ ID NO: 8749; SEQ ID NO: 8750; SEQ ID NO: 8751; SEQ ID NO: 8752; SEQ ID NO: 8753; SEQ ID NO: 8754; SEQ ID NO: 8755; SEQ ID NO: 8756; SEQ ID NO: 8757; SEQ ID NO: 8758; SEQ ID NO: 8759; SEQ ID NO: 8760; SEQ ID NO: 8761; SEQ ID NO: 8762; SEQ ID NO: 8763; SEQ ID NO: 8764; SEQ ID NO: 8765; SEQ ID NO: 8766; SEQ ID NO: 8767; SEQ ID NO: 8768; SEQ ID NO: 8769; SEQ ID NO: 8770; SEQ ID NO: 8771; SEQ ID NO: 8772; SEQ ID NO: 8773; SEQ ID NO: 8774; SEQ ID NO: 8775; SEQ ID NO: 8776; SEQ ID NO: 8777; SEQ ID NO: 8778; SEQ ID NO: 8779; SEQ ID NO: 8780; SEQ ID NO: 8781; SEQ ID NO: 8782; SEQ ID NO: 8783; SEQ ID NO: 8784; SEQ ID NO: 8785; SEQ ID NO: 8786; SEQ ID NO: 8787; SEQ ID NO: 8788; SEQ ID NO: 8789; SEQ ID NO: 8790; SEQ ID NO: 8791; SEQ ID NO: 8792; SEQ ID NO: 8793; SEQ ID NO: 8794; SEQ ID NO: 8795; SEQ ID NO: 8796; SEQ ID NO: 8797; SEQ ID NO: 8798; SEQ ID NO: 8799; SEQ ID NO: 8800; SEQ ID NO: 8801; SEQ ID NO: 8802; SEQ ID NO: 8803; SEQ ID NO: 8804; SEQ ID NO: 8805; SEQ ID NO: 8806; SEQ ID NO: 8807; SEQ ID NO: 8808; SEQ ID NO: 8809; SEQ ID NO: 8810; SEQ ID NO: 8811; SEQ ID NO: 8812; SEQ ID NO: 8813; SEQ ID NO: 8814; SEQ ID NO: 8815; SEQ ID NO: 8816; SEQ ID NO: 8817; SEQ ID NO: 8818; SEQ ID NO: 8819; SEQ ID NO: 8820; SEQ ID NO: 8821; SEQ ID NO: 8822; SEQ ID NO: 8823; SEQ ID NO: 8824; SEQ ID NO: 8825; SEQ ID NO: 8826; SEQ ID NO: 8827; SEQ ID NO: 8828; SEQ ID NO: 8829; SEQ ID NO: 8830; SEQ ID NO: 8831; SEQ ID NO: 8832; SEQ ID NO: 8833; SEQ ID NO: 8834; SEQ ID NO: 8835; SEQ ID NO: 8836; SEQ ID NO: 8837; SEQ ID NO: 8838; SEQ ID NO: 8839; SEQ ID NO: 8840; SEQ ID NO: 8841; SEQ ID NO: 8842; SEQ ID NO: 8843; SEQ ID NO: 8844; SEQ ID NO: 8845; SEQ ID NO: 8846; SEQ ID NO: 8847; SEQ ID NO: 8848; SEQ ID NO: 8849; SEQ ID NO: 8850; SEQ ID NO: 8851; SEQ ID NO: 8852; SEQ ID NO: 8853; SEQ ID NO: 8854; SEQ ID NO: 8855; SEQ ID NO: 8856; SEQ ID NO: 8857; SEQ ID NO: 8858; SEQ ID NO: 8859; SEQ ID NO: 8860; SEQ ID NO: 8861; SEQ ID NO: 8862; SEQ ID NO: 8863; SEQ ID NO: 8864; SEQ ID NO: 8865; SEQ ID NO: 8866; SEQ ID NO: 8867; SEQ ID NO: 8868; SEQ ID NO: 8869; SEQ ID NO: 8870; SEQ ID NO: 8871; SEQ ID NO: 8872; SEQ ID NO: 8873; SEQ ID NO: 8874; SEQ ID NO: 8875; SEQ ID NO: 8876; SEQ ID NO: 8877; SEQ ID NO: 9705; SEQ ID NO: 9706; SEQ ID NO: 9707; SEQ ID NO: 9708; SEQ ID NO: 9709; SEQ ID NO: 9710; SEQ ID NO: 9711; SEQ ID NO: 9712; SEQ ID NO: 9713; SEQ ID NO: 9714; SEQ ID NO: 9715; SEQ ID NO: 9716; SEQ ID NO: 9717; SEQ ID NO: 9718; SEQ ID NO: 9719; SEQ ID NO: 9720; SEQ ID NO: 9721; SEQ ID NO: 9722; SEQ ID NO: 9723; SEQ ID NO: 9724; SEQ ID NO: 9725; SEQ ID NO: 9726; SEQ ID NO: 9727; SEQ ID NO: 9728; SEQ ID NO: 9729; SEQ ID NO: 9730; SEQ ID NO: 9731; SEQ ID NO: 9732 and SEQ ID NO: 9733.  
     [0136] In one embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in energy metabolism or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 6338; SEQ ID NO: 6339; SEQ ID NO: 6340; SEQ ID NO: 6341; SEQ ID NO: 6342; SEQ ID NO: 6343; SEQ ID NO: 6344; SEQ ID NO: 6345; SEQ ID NO: 6346; SEQ ID NO: 6347; SEQ ID NO: 6348; SEQ ID NO: 6349; SEQ ID NO: 6350; SEQ ID NO: 6351; SEQ ID NO: 6352; SEQ ID NO: 6353; SEQ ID NO: 6354; SEQ ID NO: 6355; SEQ ID NO: 6356; SEQ ID NO: 6357; SEQ ID NO: 6358; SEQ ID NO: 6359; SEQ ID NO: 6360; SEQ ID NO: 6361; SEQ ID NO: 6362; SEQ ID NO: 6363; SEQ ID NO: 6364; SEQ ID NO: 6365; SEQ ID NO: 6366; SEQ ID NO: 6367; SEQ ID NO: 6368; SEQ ID NO: 6369; SEQ ID NO: 6370; SEQ ID NO: 6371; SEQ ID NO: 6372; SEQ ID NO: 6373; SEQ ID NO: 6374; SEQ ID NO: 6375; SEQ ID NO: 6376; SEQ ID NO: 6377; SEQ ID NO: 6378; SEQ ID NO: 6379; SEQ ID NO: 6380; SEQ ID NO: 6381; SEQ ID NO: 6382; SEQ ID NO: 6383; SEQ ID NO: 6384; SEQ ID NO: 6385; SEQ ID NO: 6386; SEQ ID NO: 6387; SEQ ID NO: 6389; SEQ ID NO: 6390; SEQ ID NO: 6391; SEQ ID NO: 6392; SEQ ID NO: 6393; SEQ ID NO: 6394; SEQ ID NO: 6395; SEQ ID NO: 6396; SEQ ID NO: 6397; SEQ ID NO: 6398; SEQ ID NO: 6399; SEQ ID NO: 6400; SEQ ID NO: 6401; SEQ ID NO: 6402; SEQ ID NO: 6403; SEQ ID NO: 6404; SEQ ID NO: 6405; SEQ ID NO: 6406; SEQ ID NO: 6407; SEQ ID NO: 6408; SEQ ID NO: 6409; SEQ ID NO: 6410; SEQ ID NO: 6411; SEQ ID NO: 6412; SEQ ID NO: 6413; SEQ ID NO: 6414; SEQ ID NO: 6415; SEQ ID NO: 6416; SEQ ID NO: 6417; SEQ ID NO: 6418; SEQ ID NO: 6419; SEQ ID NO: 6420; SEQ ID NO: 6421; SEQ ID NO: 6422; SEQ ID NO: 6423; SEQ ID NO: 6424; SEQ ID NO: 6425; SEQ ID NO: 6426; SEQ ID NO: 6427; SEQ ID NO: 6428; SEQ ID NO: 6429; SEQ ID NO: 6430; SEQ ID NO: 6431; SEQ ID NO: 6432; SEQ ID NO: 6433; SEQ ID NO: 6434; SEQ ID NO: 6435; SEQ ID NO: 6436; SEQ ID NO: 6437; SEQ ID NO: 6438; SEQ ID NO: 6439; SEQ ID NO: 6440; SEQ ID NO: 6441; SEQ ID NO: 6442; SEQ ID NO: 6443; SEQ ID NO: 6444; SEQ ID NO: 6445; SEQ ID NO: 6446; SEQ ID NO: 6447; SEQ ID NO: 6448; SEQ ID NO: 6449; SEQ ID NO: 6450; SEQ ID NO: 6451; SEQ ID NO: 6452; SEQ ID NO: 6453; SEQ ID NO: 6454; SEQ ID NO: 6455; SEQ ID NO: 6456; SEQ ID NO: 6457; SEQ ID NO: 6458; SEQ ID NO: 6459; SEQ ID NO: 6460; SEQ ID NO: 6461; SEQ ID NO: 6462; SEQ ID NO: 6463; SEQ ID NO: 6464; SEQ ID NO: 6465; SEQ ID NO: 6466; SEQ ID NO: 6467; SEQ ID NO: 6468; SEQ ID NO: 6469; SEQ ID NO: 6470; SEQ ID NO: 6471; SEQ ID NO: 6472; SEQ ID NO: 6473; SEQ ID NO: 6474; SEQ ID NO: 6475; SEQ ID NO: 6476; SEQ ID NO: 6477; SEQ ID NO: 6478; SEQ ID NO: 6479; SEQ ID NO: 6480; SEQ ID NO: 6481; SEQ ID NO: 6482; SEQ ID NO: 6483; SEQ ID NO: 6484; SEQ ID NO: 6485; SEQ ID NO: 6486; SEQ ID NO: 6487; SEQ ID NO: 9705 and SEQ ID NO: 9706.  
     [0137] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in amino acid metabolism or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 6488; SEQ ID NO: 6489; SEQ ID NO: 6490; SEQ ID NO: 6491; SEQ ID NO: 6492; SEQ ID NO: 6493; SEQ ID NO: 6494; SEQ ID NO: 6495; SEQ ID NO: 6496; SEQ ID NO: 6497; SEQ ID NO: 6498; SEQ ID NO: 6499; SEQ ID NO: 6500; SEQ ID NO: 6501; SEQ ID NO: 6502; SEQ ID NO: 6503; SEQ ID NO: 6504; SEQ ID NO: 6505; SEQ ID NO: 6506; SEQ ID NO: 6507; SEQ ID NO: 6508; SEQ ID NO: 6509; SEQ ID NO: 6510; SEQ ID NO: 6511; SEQ ID NO: 6512; SEQ ID NO: 6513; SEQ ID NO: 6514; SEQ ID NO: 6515; SEQ ID NO: 6516; SEQ ID NO: 6517; SEQ ID NO: 6518; SEQ ID NO: 6519; SEQ ID NO: 6520; SEQ ID NO: 6521; SEQ ID NO: 6522; SEQ ID NO: 6523; SEQ ID NO: 6524; SEQ ID NO: 6525; SEQ ID NO: 6526, SEQ ID NO: 6527; SEQ ID NO: 6528; SEQ ID NO: 6529; SEQ ID NO: 6530; SEQ ID NO: 6531; SEQ ID NO: 6532; SEQ ID NO: 6533; SEQ ID NO: 6534; SEQ ID NO: 6535; SEQ ID NO: 6536; SEQ ID NO: 6537; SEQ ID NO: 6538; SEQ ID NO: 6539; SEQ ID NO: 6540; SEQ ID NO: 6541; SEQ ID NO: 6542; SEQ ID NO: 6543; SEQ ID NO: 6544; SEQ ID NO: 6545; SEQ ID NO: 6546; SEQ ID NO: 6547; SEQ ID NO: 6548; SEQ ID NO: 6549; SEQ ID NO: 6550; SEQ ID NO: 6551; SEQ ID NO: 6552; SEQ ID NO: 6553; SEQ ID NO: 6554; SEQ ID NO: 6555; SEQ ID NO: 6556; SEQ ID NO: 6557; SEQ ID NO: 6558; SEQ ID NO: 6559; SEQ ID NO: 6560; SEQ ID NO: 6561; SEQ ID NO: 6562; SEQ ID NO: 6563; SEQ ID NO: 6564; SEQ ID NO: 6565; SEQ ID NO: 6566; SEQ ID NO: 6567; SEQ ID NO: 6568; SEQ ID NO: 6569; SEQ ID NO: 6570; SEQ ID NO: 6571; SEQ ID NO: 6572; SEQ ID NO: 6573; SEQ ID NO: 6574; SEQ ID NO: 6575; SEQ ID NO: 6576; SEQ ID NO: 6577; SEQ ID NO: 6578; SEQ ID NO: 6579; SEQ ID NO: 6580; SEQ ID NO: 6581; SEQ ID NO: 6582; SEQ ID NO: 6583; SEQ ID NO: 6584; SEQ ID NO: 6585; SEQ ID NO: 6586; SEQ ID NO: 6587; SEQ ID NO: 6588; SEQ ID NO: 6589; SEQ ID NO: 6590; SEQ ID NO: 6591; SEQ ID NO: 6592; SEQ ID NO: 6593; SEQ ID NO: 6594; SEQ ID NO: 6595; SEQ ID NO: 6596; SEQ ID NO: 6597; SEQ ID NO: 6598; SEQ ID NO: 6599; SEQ ID NO: 6600; SEQ ID NO: 6601; SEQ ID NO: 6602; SEQ ID NO: 6603; SEQ ID NO: 6604; SEQ ID NO: 6605; SEQ ID NO: 6606; SEQ ID NO: 6607; SEQ ID NO: 6608; SEQ ID NO: 6609; SEQ ID NO: 6610; SEQ ID NO: 6611; SEQ ID NO: 6612; SEQ ID NO: 6613; SEQ ID NO: 6614; SEQ ID NO: 6615; SEQ ID NO: 6616; SEQ ID NO: 6617; SEQ ID NO: 6618; SEQ ID NO: 6619; SEQ ID NO: 6620; SEQ ID NO: 6621; SEQ ID NO: 6622; SEQ ID NO: 6623; SEQ ID NO: 6624; SEQ ID NO: 6625; SEQ ID NO: 6626; SEQ ID NO: 6627; SEQ ID NO: 6628; SEQ ID NO: 6629; SEQ ID NO: 6630; SEQ ID NO: 9707 and SEQ ID NO: 9708.  
     [0138] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in cofactor metabolism or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 6631; SEQ ID NO: 6632; SEQ ID NO: 6633; SEQ ID NO: 6634; SEQ ID NO: 6635; SEQ ID NO: 6636; SEQ ID NO: 6637; SEQ ID NO: 6638; SEQ ID NO: 6639; SEQ ID NO: 6640; SEQ ID NO: 6641; SEQ ID NO: 6642; SEQ ID NO: 6643; SEQ ID NO: 6644; SEQ ID NO: 6645; SEQ ID NO: 6646; SEQ ID NO: 6647; SEQ ID NO: 6648; SEQ ID NO: 6649; SEQ ID NO: 6650; SEQ ID NO: 6651; SEQ ID NO: 6652; SEQ ID NO: 6653; SEQ ID NO: 6654; SEQ ID NO: 6655; SEQ ID NO: 6656; SEQ ID NO: 6657; SEQ ID NO: 6658; SEQ ID NO: 6659; SEQ ID NO: 6660; SEQ ID NO: 6661; SEQ ID NO: 6662; SEQ ID NO: 6663; SEQ ID NO: 6664; SEQ ID NO: 6665; SEQ ID NO: 6666; SEQ ID NO: 6667; SEQ ID NO: 6668; SEQ ID NO: 6669; SEQ ID NO: 6670; SEQ ID NO: 6671; SEQ ID NO: 6672; SEQ ID NO: 6673; SEQ ID NO: 6674; SEQ ID NO: 6675; SEQ ID NO: 6676; SEQ ID NO: 6677; SEQ ID NO: 6678; SEQ ID NO: 6679; SEQ ID NO: 6680; SEQ ID NO: 6681; SEQ ID NO: 6682; SEQ ID NO: 6683; SEQ ID NO: 6684; SEQ ID NO: 6685; SEQ ID NO: 6686; SEQ ID NO: 6687; SEQ ID NO: 6688; SEQ ID NO: 6689; SEQ ID NO: 6690; SEQ ID NO: 6691; SEQ ID NO: 6692; SEQ ID NO: 6693; SEQ ID NO: 6694; SEQ ID NO: 6695; SEQ ID NO: 6696; SEQ ID NO: 6697; SEQ ID NO: 6698; SEQ ID NO: 6699; SEQ ID NO: 6700; SEQ ID NO: 6701; SEQ ID NO: 6702; SEQ ID NO: 6703; SEQ ID NO: 6704; SEQ ID NO: 6705; SEQ ID NO: 6706; SEQ ID NO: 6707; SEQ ID NO: 6708; SEQ ID NO: 6709; SEQ ID NO: 6710; SEQ ID NO: 6711; SEQ ID NO: 6712; SEQ ID NO: 6713; SEQ ID NO: 6714; SEQ ID NO: 6715; SEQ ID NO: 6716; SEQ ID NO: 6717; SEQ ID NO: 6718; SEQ ID NO: 6719; SEQ ID NO: 6720; SEQ ID NO: 6721; SEQ ID NO: 6722; SEQ ID NO: 6723; SEQ ID NO: 6724; SEQ ID NO: 6725; SEQ ID NO: 6726; SEQ ID NO: 6727; SEQ ID NO: 6728; SEQ ID NO: 6729; SEQ ID NO: 6730; SEQ ID NO: 6731; SEQ ID NO: 6732; SEQ ID NO: 6733; SEQ ID NO: 6734; SEQ ID NO: 6735; SEQ ID NO: 6736; SEQ ID NO: 6737; SEQ ID NO: 6738; SEQ ID NO: 6739; SEQ ID NO: 6740; SEQ ID NO: 6741; SEQ ID NO: 6742; SEQ ID NO: 6743; SEQ ID NO: 6744; SEQ ID NO: 6745; SEQ ID NO: 6746; SEQ ID NO: 6747; SEQ ID NO: 6748; SEQ ID NO: 6749, SEQ ID NO: 6750; SEQ ID NO: 6751; SEQ ID NO: 6752; SEQ ID NO: 6753; SEQ ID NO: 6754; SEQ ID NO: 6755; SEQ ID NO: 6756; SEQ ID NO: 6757; SEQ ID NO: 6758; SEQ ID NO: 6759; SEQ ID NO: 6760; SEQ ID NO: 6761; SEQ ID NO: 6762; SEQ ID NO: 6763; SEQ ID NO: 6764; SEQ ID NO: 6765; SEQ ID NO: 6766; SEQ ID NO: 6767 and SEQ ID NO: 9709.  
     [0139] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in carbohydrate metabolism or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 6768; SEQ ID NO: 6769; SEQ ID NO: 6770; SEQ ID NO: 6771; SEQ ID NO: 6772; SEQ ID NO: 6773; SEQ ID NO: 6774; SEQ ID NO: 6775; SEQ ID NO: 6776; SEQ ID NO: 6777; SEQ ID NO: 6778; SEQ ID NO: 6779; SEQ ID NO: 6780; SEQ ID NO: 6781; SEQ ID NO: 6782; SEQ ID NO: 6783; SEQ ID NO: 6784; SEQ ID NO: 6785; SEQ ID NO: 6786; SEQ ID NO: 6787; SEQ ID NO: 6788; SEQ ID NO: 6789; SEQ ID NO: 6790; SEQ ID NO: 6791; SEQ ID NO: 6792; SEQ ID NO: 6793; SEQ ID NO: 6794; SEQ ID NO: 6795; SEQ ID NO: 6796; SEQ ID NO: 6797; SEQ ID NO: 6798; SEQ ID NO: 6799; SEQ ID NO: 6800; SEQ ID NO: 6801; SEQ ID NO: 6802; SEQ ID NO: 6803; SEQ ID NO: 6804; SEQ ID NO: 6805; SEQ ID NO: 6806; SEQ ID NO: 6807; SEQ ID NO: 6808; SEQ ID NO: 6809; SEQ ID NO: 6810; SEQ ID NO: 6811; SEQ ID NO: 6812; SEQ ID NO: 6813; SEQ ID NO: 6814; SEQ ID NO: 6815; SEQ ID NO: 6816; SEQ ID NO: 6817; SEQ ID NO: 6818; SEQ ID NO: 6819; SEQ ID NO: 6820; SEQ ID NO: 6821; SEQ ID NO: 6822; SEQ ID NO: 6823; SEQ ID NO: 6824; SEQ ID NO: 6825; SEQ ID NO: 6826; SEQ ID NO: 6827; SEQ ID NO: 6828; SEQ ID NO: 6829; SEQ ID NO: 6830; SEQ ID NO: 6831; SEQ ID NO: 6832; SEQ ID NO: 6833; SEQ ID NO: 6834; SEQ ID NO: 6835; SEQ ID NO: 6836; SEQ ID NO: 6837; SEQ ID NO: 6838; SEQ ID NO: 9710 and SEQ ID NO: 9711.  
     [0140] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in nucleic acid metabolism or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 6839; SEQ ID NO: 6840; SEQ ID NO: 6841; SEQ ID NO: 6842; SEQ ID NO: 6843; SEQ ID NO: 6844; SEQ ID NO: 6845; SEQ ID NO: 6846; SEQ ID NO: 6847; SEQ ID NO: 6848; SEQ ID NO: 6849; SEQ ID NO: 6850; SEQ ID NO: 6851; SEQ ID NO: 6852; SEQ ID NO: 6853; SEQ ID NO: 6854; SEQ ID NO: 6855; SEQ ID NO: 6856; SEQ ID NO: 6857; SEQ ID NO: 6858; SEQ ID NO: 6859; SEQ ID NO: 6860; SEQ ID NO: 6861; SEQ ID NO: 6862; SEQ ID NO: 6863; SEQ ID NO: 6864; SEQ ID NO: 6865; SEQ ID NO: 6866; SEQ ID NO: 6867; SEQ ID NO: 6868; SEQ ID NO: 6869; SEQ ID NO: 6870; SEQ ID NO: 6871; SEQ ID NO: 6872; SEQ ID NO: 6873; SEQ ID NO: 6874; SEQ ID NO: 6875; SEQ ID NO: 6876; SEQ ID NO: 6877; SEQ ID NO: 6878; SEQ ID NO: 6879; SEQ ID NO: 6880; SEQ ID NO: 6881; SEQ ID NO: 6882; SEQ ID NO: 6883; SEQ ID NO: 6884; SEQ ID NO: 6885; SEQ ID NO: 6886; SEQ ID NO: 6887; SEQ ID NO: 6888; SEQ ID NO: 6889; SEQ ID NO: 6890; SEQ ID NO: 6891; SEQ ID NO: 6892; SEQ ID NO: 6893; SEQ ID NO: 6894; SEQ ID NO: 6895; SEQ ID NO: 6896; SEQ ID NO: 6897; SEQ ID NO: 6898; SEQ ID NO: 6899; SEQ ID NO: 6900; SEQ ID NO: 6901; SEQ ID NO: 6902; SEQ ID NO: 6903; SEQ ID NO: 6904; SEQ ID NO: 6905; SEQ ID NO: 6906; SEQ ID NO: 6907; SEQ ID NO: 6908; SEQ ID NO: 6909; SEQ ID NO: 6910; SEQ ID NO: 6911; SEQ ID NO: 6912; SEQ ID NO: 6913; SEQ ID NO: 6914; SEQ ID NO: 6915; SEQ ID NO: 6916; SEQ ID NO: 6917; SEQ ID NO: 6918; SEQ ID NO: 6919; SEQ ID NO: 6920; SEQ ID NO: 6921; SEQ ID NO: 6922; SEQ ID NO: 6923; SEQ ID NO: 6924; SEQ ID NO: 6925; SEQ ID NO: 6926; SEQ ID NO: 6927; SEQ ID NO: 6928; SEQ ID NO: 6929; SEQ ID NO: 6930; SEQ ID NO: 6931; SEQ ID NO: 6932; SEQ ID NO: 6933; SEQ ID NO: 6934; SEQ ID NO: 6935; SEQ ID NO: 6936; SEQ ID NO: 6937; SEQ ID NO: 6938; SEQ ID NO: 6939; SEQ ID NO: 6940; SEQ ID NO: 6941; SEQ ID NO: 6942; SEQ ID NO: 6943; SEQ ID NO: 6944 and SEQ ID NO: 9712.  
     [0141] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in lipid metabolism or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 6945; SEQ ID NO: 6946; SEQ ID NO: 6947; SEQ ID NO: 6948; SEQ ID NO: 6949; SEQ ID NO: 6950; SEQ ID NO: 6951; SEQ ID NO: 6952; SEQ ID NO: 6953; SEQ ID NO: 6954; SEQ ID NO: 6955; SEQ ID NO: 6956; SEQ ID NO: 6957; SEQ ID NO: 6958; SEQ ID NO: 6959; SEQ ID NO: 6960; SEQ ID NO: 6961; SEQ ID NO: 6962; SEQ ID NO: 6963; SEQ ID NO: 6964; SEQ ID NO: 6965; SEQ ID NO: 6966; SEQ ID NO: 6967; SEQ ID NO: 6968; SEQ ID NO: 6969; SEQ ID NO: 6970; SEQ ID NO: 6971; SEQ ID NO: 6972; SEQ ID NO: 6973; SEQ ID NO: 6974; SEQ ID NO: 6975; SEQ ID NO: 6976; SEQ ID NO: 6977; SEQ ID NO: 6978; SEQ ID NO: 6979; SEQ ID NO: 6980; SEQ ID NO: 9713 and SEQ ID NO: 9714.  
     [0142] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in mRNA translation and ribosome biogenesis or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 6981; SEQ ID NO: 6982; SEQ ID NO: 6983; SEQ ID NO: 6984; SEQ ID NO: 6985; SEQ ID NO: 6986; SEQ ID NO: 6987; SEQ ID NO: 6988; SEQ ID NO: 6989; SEQ ID NO: 6990; SEQ ID NO: 6991; SEQ ID NO: 6992; SEQ ID NO: 6993; SEQ ID NO: 6994; SEQ ID NO: 6995; SEQ ID NO: 6996; SEQ ID NO: 6997; SEQ ID NO: 6998; SEQ ID NO: 6999; SEQ ID NO: 7000; SEQ ID NO: 7001; SEQ ID NO: 7002; SEQ ID NO: 7003; SEQ ID NO: 7004; SEQ ID NO: 7005; SEQ ID NO: 7006; SEQ ID NO: 7007; SEQ ID NO: 7008; SEQ ID NO: 7009; SEQ ID NO: 7010; SEQ ID NO: 7011; SEQ ID NO: 7012; SEQ ID NO: 7013; SEQ ID NO: 7014; SEQ ID NO: 7015; SEQ ID NO: 7016; SEQ ID NO: 7017; SEQ ID NO: 7018; SEQ ID NO: 7019; SEQ ID NO: 7020; SEQ ID NO: 7021; SEQ ID NO: 7022; SEQ ID NO: 7023; SEQ ID NO: 7024; SEQ ID NO: 7025; SEQ ID NO: 7026; SEQ ID NO: 7027; SEQ ID NO: 7028; SEQ ID NO: 7029; SEQ ID NO: 7030; SEQ ID NO: 7031; SEQ ID NO: 7032; SEQ ID NO: 7033; SEQ ID NO: 7034; SEQ ID NO: 7035; SEQ ID NO: 7036; SEQ ID NO: 7037; SEQ ID NO: 7038; SEQ ID NO: 7039; SEQ ID NO: 7040; SEQ ID NO: 7041; SEQ ID NO: 7042; SEQ ID NO: 7043; SEQ ID NO: 7044; SEQ ID NO: 7045; SEQ ID NO: 7046; SEQ ID NO: 7047; SEQ ID NO: 7048; SEQ ID NO: 7049; SEQ ID NO: 7050; SEQ ID NO: 7051; SEQ ID NO: 7052; SEQ ID NO: 7053; SEQ ID NO: 7054; SEQ ID NO: 7055; SEQ ID NO: 7056; SEQ ID NO: 7057; SEQ ID NO: 7058; SEQ ID NO: 7059; SEQ ID NO: 7060; SEQ ID NO: 7061; SEQ ID NO: 7062; SEQ ID NO: 7063; SEQ ID NO: 7064; SEQ ID NO: 7065; SEQ ID NO: 7066; SEQ ID NO: 7067; SEQ ID NO: 7068; SEQ ID NO: 7069; SEQ ID NO: 7070; SEQ ID NO: 7071; SEQ ID NO: 7072; SEQ ID NO: 7073; SEQ ID NO: 7074; SEQ ID NO: 7075; SEQ ID NO: 7076; SEQ ID NO: 7077; SEQ ID NO: 7078; SEQ ID NO: 7079; SEQ ID NO: 7080; SEQ ID NO: 7081; SEQ ID NO: 7082; SEQ ID NO: 7083; SEQ ID NO: 7084; SEQ ID NO: 7085; SEQ ID NO: 7086; SEQ ID NO: 7087; SEQ ID NO: 7088; SEQ ID NO: 7089; SEQ ID NO: 7090; SEQ ID NO: 7091; SEQ ID NO: 7092; SEQ ID NO: 7093; SEQ ID NO: 7094; SEQ ID NO: 7095; SEQ ID NO: 7096; SEQ ID NO: 7097; SEQ ID NO: 7098; SEQ ID NO: 7099; SEQ ID NO: 7100; SEQ ID NO: 7101; SEQ ID NO: 7102; SEQ ID NO: 7103; SEQ ID NO: 7104; SEQ ID NO: 7105; SEQ ID NO: 7106; SEQ ID NO: 7107; SEQ ID NO: 7108; SEQ ID NO: 7109; SEQ ID NO: 7110; SEQ ID NO: 7111; SEQ ID NO: 7112; SEQ ID NO: 7113; SEQ ID NO: 7114; SEQ ID NO: 7115; SEQ ID NO: 7116; SEQ ID NO: 7117; SEQ ID NO: 7118; SEQ ID NO: 7119; SEQ ID NO: 7120; SEQ ID NO: 7121; SEQ ID NO: 7122; SEQ ID NO: 7123; SEQ ID NO: 7124; SEQ ID NO: 7125; SEQ ID NO: 7126; SEQ ID NO: 7127; SEQ ID NO: 7128; SEQ ID NO: 7129; SEQ ID NO: 7130; SEQ ID NO: 7131; SEQ ID NO: 7132; SEQ ID NO: 7133; SEQ ID NO: 7134; SEQ ID NO: 7135; SEQ ID NO: 7136; SEQ ID NO: 7137; SEQ ID NO: 7138; SEQ ID NO: 7139; SEQ ID NO: 7140; SEQ ID NO: 7141; SEQ ID NO: 7142; SEQ ID NO: 7143; SEQ ID NO: 7144; SEQ ID NO: 7145; SEQ ID NO: 7146; SEQ ID NO: 7147; SEQ ID NO: 7148; SEQ ID NO: 7149; SEQ ID NO: 7150; SEQ ID NO: 7151; SEQ ID NO: 7152; SEQ ID NO: 7153; SEQ ID NO: 7154; SEQ ID NO: 7155; SEQ ID NO: 7156; SEQ ID NO: 7157; SEQ ID NO: 7158; SEQ ID NO: 7159; SEQ ID NO: 7160; SEQ ID NO: 7161; SEQ ID NO: 7162; SEQ ID NO: 7163; SEQ ID NO: 7164; SEQ ID NO: 7165; SEQ ID NO: 7166; SEQ ID NO: 7167; SEQ ID NO: 7168; SEQ ID NO: 7169; SEQ ID NO: 7170; SEQ ID NO: 7171; SEQ ID NO: 7172; SEQ ID NO: 7173; SEQ ID NO: 7174; SEQ ID NO: 7175; SEQ ID NO: 7176; SEQ ID NO: 7177; SEQ ID NO: 7178; SEQ ID NO: 7179; SEQ ID NO: 7180; SEQ ID NO: 7181; SEQ ID NO: 7182; SEQ ID NO: 7183; SEQ ID NO: 7184; SEQ ID NO: 7185; SEQ ID NO: 7186; SEQ ID NO: 7187; SEQ ID NO: 7188; SEQ ID NO: 7189; SEQ ID NO: 7190; SEQ ID NO: 7191; SEQ ID NO: 7192; SEQ ID NO: 7193; SEQ ID NO: 7194; SEQ ID NO: 7195; SEQ ID NO: 7196; SEQ ID NO: 7197; SEQ ID NO: 7198; SEQ ID NO: 7199; SEQ ID NO: 7200; SEQ ID NO: 9715 and SEQ ID NO: 9716.  
     [0143] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in genome replication, transcription, recombination and repair or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 7201; SEQ ID NO: 7202; SEQ ID NO: 7203; SEQ ID NO: 7204; SEQ ID NO: 7205; SEQ ID NO: 7206; SEQ ID NO: 7207; SEQ ID NO: 7208; SEQ ID NO: 7209; SEQ ID NO: 7210; SEQ ID NO: 7211; SEQ ID NO: 7212; SEQ ID NO: 7213; SEQ ID NO: 7214; SEQ ID NO: 7215; SEQ ID NO: 7216; SEQ ID NO: 7217; SEQ ID NO: 7218; SEQ ID NO: 7219; SEQ ID NO: 7220; SEQ ID NO: 7221; SEQ ID NO: 7222; SEQ ID NO: 7223; SEQ ID NO: 7224; SEQ ID NO: 7225; SEQ ID NO: 7226; SEQ ID NO: 7227; SEQ ID NO: 7228; SEQ ID NO: 7229; SEQ ID NO: 7230; SEQ ID NO: 7231; SEQ ID NO: 7232; SEQ ID NO: 7233; SEQ ID NO: 7234; SEQ ID NO: 7235; SEQ ID NO: 7236; SEQ ID NO: 7237; SEQ ID NO: 7238; SEQ ID NO: 7239; SEQ ID NO: 7240; SEQ ID NO: 7241; SEQ ID NO: 7242; SEQ ID NO: 7243; SEQ ID NO: 7244; SEQ ID NO: 7245; SEQ ID NO: 7246; SEQ ID NO: 7247; SEQ ID NO: 7248; SEQ ID NO: 7249; SEQ ID NO: 7250; SEQ ID NO: 7251; SEQ ID NO: 7252; SEQ ID NO: 7253; SEQ ID NO: 7254; SEQ ID NO: 7255; SEQ ID NO: 7256; SEQ ID NO: 7257; SEQ ID NO: 7258; SEQ ID NO: 7259; SEQ ID NO: 7260; SEQ ID NO: 7261; SEQ ID NO: 7262; SEQ ID NO: 7263; SEQ ID NO: 7264; SEQ ID NO: 7265; SEQ ID NO: 7266; SEQ ID NO: 7267; SEQ ID NO: 7268; SEQ ID NO: 7269; SEQ ID NO: 7270; SEQ ID NO: 7271; SEQ ID NO: 7272; SEQ ID NO: 7273; SEQ ID NO: 7274; SEQ ID NO: 7275; SEQ ID NO: 7276; SEQ ID NO: 7277; SEQ ID NO: 7278; SEQ ID NO: 7279; SEQ ID NO: 7280; SEQ ID NO: 7281; SEQ ID NO: 7282; SEQ ID NO: 7283; SEQ ID NO: 7284; SEQ ID NO: 7285; SEQ ID NO: 7286; SEQ ID NO: 7287; SEQ ID NO: 7288; SEQ ID NO: 7289; SEQ ID NO: 7290; SEQ ID NO: 7291; SEQ ID NO: 7292; SEQ ID NO: 7293; SEQ ID NO: 7294; SEQ ID NO: 7295; SEQ ID NO: 7296; SEQ ID NO: 7297; SEQ ID NO: 7298; SEQ ID NO: 7299; SEQ ID NO: 7300; SEQ ID NO: 7301; SEQ ID NO: 7302; SEQ ID NO: 7303; SEQ ID NO: 7304; SEQ ID NO: 7305; SEQ ID NO: 7306; SEQ ID NO: 7307; SEQ ID NO: 7308; SEQ ID NO: 7309; SEQ ID NO: 7310; SEQ ID NO: 7311; SEQ ID NO: 7312; SEQ ID NO: 7313; SEQ ID NO: 7314; SEQ ID NO: 7315; SEQ ID NO: 7316; SEQ ID NO: 7317; SEQ ID NO: 7318; SEQ ID NO: 7319; SEQ ID NO: 7320; SEQ ID NO: 7321; SEQ ID NO: 7322; SEQ ID NO: 7323; SEQ ID NO: 7324; SEQ ID NO: 7325; SEQ ID NO: 7326; SEQ ID NO: 7327; SEQ ID NO: 7328; SEQ ID NO: 7329; SEQ ID NO: 7330; SEQ ID NO: 7331; SEQ ID NO: 7332; SEQ ID NO: 7333; SEQ ID NO: 7334; SEQ ID NO: 7335; SEQ ID NO: 7336; SEQ ID NO: 7337; SEQ ID NO: 7338; SEQ ID NO: 7339; SEQ ID NO: 7340; SEQ ID NO: 7341; SEQ ID NO: 7342; SEQ ID NO: 7343; SEQ ID NO: 7344; SEQ ID NO: 7345; SEQ ID NO: 7346; SEQ ID NO: 7347; SEQ ID NO: 7348; SEQ ID NO: 7349; SEQ ID NO: 7350; SEQ ID NO: 7351; SEQ ID NO: 7352; SEQ ID NO: 7353; SEQ ID NO: 7354; SEQ ID NO: 7355; SEQ ID NO: 7356; SEQ ID NO: 7357; SEQ ID NO: 7358; SEQ ID NO: 7359; SEQ ID NO: 7360; SEQ ID NO: 7361; SEQ ID NO: 7362; SEQ ID NO: 7363; SEQ ID NO: 7364; SEQ ID NO: 7365; SEQ ID NO: 7366; SEQ ID NO: 7367; SEQ ID NO: 7368; SEQ ID NO: 7369; SEQ ID NO: 7370; SEQ ID NO: 7371; SEQ ID NO: 7372; SEQ ID NO: 7373; SEQ ID NO: 7374; SEQ ID NO: 7375; SEQ ID NO: 7376; SEQ ID NO: 7377; SEQ ID NO: 7378; SEQ ID NO: 7379; SEQ ID NO: 7380; SEQ ID NO: 7381; SEQ ID NO: 7382; SEQ ID NO: 7383; SEQ ID NO: 7384; SEQ ID NO: 7385; SEQ ID NO: 7386; SEQ ID NO: 7387; SEQ ID NO: 7388; SEQ ID NO: 7389; SEQ ID NO: 7390; SEQ ID NO: 7391; SEQ ID NO: 7392; SEQ ID NO: 7393; SEQ ID NO: 7394; SEQ ID NO: 7395; SEQ ID NO: 7396; SEQ ID NO: 7397; SEQ ID NO: 7398; SEQ ID NO: 7399; SEQ ID NO: 7400; SEQ ID NO: 7401; SEQ ID NO: 7402; SEQ ID NO: 7403; SEQ ID NO: 7404; SEQ ID NO: 7405; SEQ ID NO: 7406; SEQ ID NO: 7407; SEQ ID NO: 7408; SEQ ID NO: 7409; SEQ ID NO: 7410; SEQ ID NO: 7411; SEQ ID NO: 7412; SEQ ID NO: 7413; SEQ ID NO: 7414; SEQ ID NO: 7415; SEQ ID NO: 7416; SEQ ID NO: 7417; SEQ ID NO: 7418; SEQ ID NO: 7419; SEQ ID NO: 7420; SEQ ID NO: 7421; SEQ ID NO: 7422; SEQ ID NO: 7423; SEQ ID NO: 7424; SEQ ID NO: 7425; SEQ ID NO: 7426; SEQ ID NO: 7427; SEQ ID NO: 7428; SEQ ID NO: 7429; SEQ ID NO: 7430; SEQ ID NO: 7431; SEQ ID NO: 7432; SEQ ID NO: 7433; SEQ ID NO: 7434; SEQ ID NO: 7435; SEQ ID NO: 7436; SEQ ID NO: 7437; SEQ ID NO: 7438; SEQ ID NO: 7439; SEQ ID NO: 7440; SEQ ID NO: 7441; SEQ ID NO: 7442; SEQ ID NO: 7443; SEQ ID NO: 7444; SEQ ID NO: 7445; SEQ ID NO: 7446; SEQ ID NO: 7447; SEQ ID NO: 7448; SEQ ID NO: 7449; SEQ ID NO: 7450; SEQ ID NO: 7451; SEQ ID NO: 7452; SEQ ID NO: 7453; SEQ ID NO: 7454; SEQ ID NO: 7455; SEQ ID NO: 7456; SEQ ID NO: 7457; SEQ ID NO: 7458; SEQ ID NO: 7459; SEQ ID NO: 7460; SEQ ID NO: 7461; SEQ ID NO: 7462; SEQ ID NO: 7463; SEQ ID NO: 7464; SEQ ID NO: 7465; SEQ ID NO: 7466; SEQ ID NO: 7467; SEQ ID NO: 7468; SEQ ID NO: 7469; SEQ ID NO: 7470; SEQ ID NO: 7471; SEQ ID NO: 7472; SEQ ID NO: 7473; SEQ ID NO: 7474; SEQ ID NO: 7475; SEQ ID NO: 7476; SEQ ID NO: 7477; SEQ ID NO: 7478; SEQ ID NO: 7479; SEQ ID NO: 7480; SEQ ID NO: 7481; SEQ ID NO: 7482; SEQ ID NO: 7483; SEQ ID NO: 7484; SEQ ID NO: 7485; SEQ ID NO: 7486; SEQ ID NO: 7487; SEQ ID NO: 7488; SEQ ID NO: 7489; SEQ ID NO: 7490; SEQ ID NO: 7491; SEQ ID NO: 7492; SEQ ID NO: 7493; SEQ ID NO: 7494; SEQ ID NO: 7495; SEQ ID NO: 7496; SEQ ID NO: 7497; SEQ ID NO: 7498; SEQ ID NO: 7499; SEQ ID NO: 7500; SEQ ID NO: 7501; SEQ ID NO: 7502; SEQ ID NO: 7503; SEQ ID NO: 7504; SEQ ID NO: 7505; SEQ ID NO: 7506; SEQ ID NO: 7507; SEQ ID NO: 7508; SEQ ID NO: 7509; SEQ ID NO:7510; SEQ ID NO: 7511; SEQ ID NO: 7512; SEQ ID NO: 7513; SEQ ID NO: 7514; SEQ ID NO: 7515; SEQ ID NO: 7516; SEQ ID NO: 7517; SEQ ID NO: 7518; SEQ ID NO: 7519; SEQ ID NO: 7520; SEQ ID NO: 7521; SEQ ID NO: 7522; SEQ ID NO: 7523; SEQ ID NO: 7524; SEQ ID NO: 7525; SEQ ID NO: 7526; SEQ ID NO: 7527; SEQ ID NO: 7528; SEQ ID NO: 7529; SEQ ID NO: 7530; SEQ ID NO: 7531; SEQ ID NO: 7532; SEQ ID NO: 7533; SEQ ID NO: 7534; SEQ ID NO: 7535; SEQ ID NO: 7536; SEQ ID NO: 7537; SEQ ID NO: 7538; SEQ ID NO: 7539; SEQ ID NO: 7540; SEQ ID NO: 7541; SEQ ID NO: 7542; SEQ ID NO: 7543; SEQ ID NO: 7544; SEQ ID NO: 7545; SEQ ID NO: 7546; SEQ ID NO: 7547; SEQ ID NO: 7548; SEQ ID NO: 7549; SEQ ID NO: 7550; SEQ ID NO: 7551; SEQ ID NO: 7552; SEQ ID NO: 7553; SEQ ID NO: 7554; SEQ ID NO: 7555; SEQ ID NO: 7556; SEQ ID NO: 7557; SEQ ID NO: 7558; SEQ ID NO: 7559; SEQ ID NO: 7560; SEQ ID NO: 7561; SEQ ID NO: 7562; SEQ ID NO: 7563; SEQ ID NO: 7564; SEQ ID NO: 7565; SEQ ID NO: 7566; SEQ ID NO: 7567; SEQ ID NO: 7568; SEQ ID NO: 7569; SEQ ID NO: 9717; SEQ ID NO: 9718; SEQ ID NO: 9719 and SEQ ID NO: 9720.  
     [0144] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in secretion and adhesion or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 7570; SEQ ID NO: 7571; SEQ ID NO: 7572; SEQ ID NO: 7573; SEQ ID NO: 7574; SEQ ID NO: 7575; SEQ ID NO: 7576; SEQ ID NO: 7577; SEQ ID NO: 7578; SEQ ID NO: 7579; SEQ ID NO: 7580; SEQ ID NO: 7581; SEQ ID NO: 7582; SEQ ID NO: 7583; SEQ ID NO: 7584; SEQ ID NO: 7585; SEQ ID NO: 7586.  
     [0145] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in outer membrane and cell wall biogenesis or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 7587; SEQ ID NO: 7588; SEQ ID NO: 7589; SEQ ID NO: 7590; SEQ ID NO: 7591; SEQ ID NO: 7592; SEQ ID NO: 7593; SEQ ID NO: 7594; SEQ ID NO: 7595; SEQ ID NO: 7596; SEQ ID NO: 7597; SEQ ID NO: 7598; SEQ ID NO: 7599; SEQ ID NO: 7600; SEQ ID NO: 7601; SEQ ID NO: 7602; SEQ ID NO: 7603; SEQ ID NO: 7604; SEQ ID NO: 7605; SEQ ID NO: 7606; SEQ ID NO: 7607; SEQ ID NO: 7608; SEQ ID NO: 7609; SEQ ID NO: 7610; SEQ ID NO: 7611; SEQ ID NO: 7612; SEQ ID NO: 7613; SEQ ID NO: 7614; SEQ ID NO: 7615; SEQ ID NO: 7616; SEQ ID NO: 7617; SEQ ID NO: 7618; SEQ ID NO: 7619; SEQ ID NO: 7620; SEQ ID NO: 7621; SEQ ID NO: 7622; SEQ ID NO: 7623; SEQ ID NO: 7624; SEQ ID NO: 7625; SEQ ID NO: 7626; SEQ ID NO: 7627; SEQ ID NO: 7628; SEQ ID NO: 7629; SEQ ID NO: 7630; SEQ ID NO: 7631; SEQ ID NO: 7632; SEQ ID NO: 7633; SEQ ID NO: 7634; SEQ ID NO: 7635; SEQ ID NO: 7636; SEQ ID NO: 7637; SEQ ID NO: 7638; SEQ ID NO: 7639; SEQ ID NO: 9721 and SEQ ID NO: 9722.  
     [0146] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in protein folding and stabilization or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 7640; SEQ ID NO: 7641; SEQ ID NO: 7642; SEQ ID NO: 7643; SEQ ID NO: 7644; SEQ ID NO: 7645; SEQ ID NO: 7646; SEQ ID NO: 7647; SEQ ID NO: 7648; SEQ ID NO: 7649; SEQ ID NO: 7650; SEQ ID NO: 7651; SEQ ID NO: 7652; SEQ ID NO: 7653; SEQ ID NO: 7654; SEQ ID NO: 7655; SEQ ID NO: 7656; SEQ ID NO: 7657; SEQ ID NO: 7658; SEQ ID NO: 7659; SEQ ID NO: 7660; SEQ ID NO: 7661; SEQ ID NO: 7662; SEQ ID NO: 7663; SEQ ID NO: 7664; SEQ ID NO: 7665; SEQ ID NO: 7666; SEQ ID NO: 7667; SEQ ID NO: 7668; SEQ ID NO: 7669; SEQ ID NO: 7670; SEQ ID NO: 7671; SEQ ID NO: 7672; SEQ ID NO: 7673; SEQ ID NO: 7674; SEQ ID NO: 7675; SEQ ID NO: 7676; SEQ ID NO: 7677; SEQ ID NO: 7678; SEQ ID NO: 7679; SEQ ID NO: 7680; SEQ ID NO: 9723 and SEQ ID NO: 9724.  
     [0147] Particularly preferred is an isolated  H. pylori  cytoplasmic polypeptide or a fragment thereof, wherein the polypeptide is encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1576; SEQ ID NO: 1577; SEQ ID NO: 1578; SEQ ID NO: 1579; SEQ ID NO: 1580; SEQ ID NO: 1581; SEQ ID NO: 1582; SEQ ID NO: 1583; SEQ ID NO: 1584; SEQ ID NO: 1585; SEQ ID NO: 1586; SEQ ID NO: 1587; SEQ ID NO: 1588; SEQ ID NO: 1589; SEQ ID NO: 1590; SEQ ID NO: 1591; SEQ ID NO: 1592; SEQ ID NO: 1593; SEQ ID NO: 1594; SEQ ID NO: 1595; SEQ ID NO: 1596; SEQ ID NO: 1597; SEQ ID NO: 1598; SEQ ID NO: 1599; SEQ ID NO: 1600; SEQ ID NO: 1601; SEQ ID NO: 1602; SEQ ID NO: 1603; SEQ ID NO: 1604; SEQ ID NO: 1605; SEQ ID NO: 1606; SEQ ID NO: 1607; SEQ ID NO: 1608; SEQ ID NO: 1609; SEQ ID NO: 1610; SEQ ID NO: 1611; SEQ ID NO: 1612; SEQ ID NO: 1613; SEQ ID NO: 1614; SEQ ID NO: 1615; SEQ ID NO: 1616; SEQ ID NO: 1617; SEQ ID NO: 1618; SEQ ID NO: 1619; SEQ ID NO: 1620; SEQ ID NO: 1621; SEQ ID NO: 1622; SEQ ID NO: 1623; SEQ ID NO: 1624; SEQ ID NO: 1625; SEQ ID NO: 1626; SEQ ID NO: 1627; SEQ ID NO: 1628; SEQ ID NO: 1629; SEQ ID NO: 1630; SEQ ID NO: 1631; SEQ ID NO: 1632; SEQ ID NO: 1633; SEQ ID NO: 1634; SEQ ID NO: 1635; SEQ ID NO: 1636; SEQ ID NO: 1637; SEQ ID NO: 1638; SEQ ID NO: 1639; SEQ ID NO: 1640; SEQ ID NO: 1641; SEQ ID NO: 1642; SEQ ID NO: 1643; SEQ ID NO: 1644; SEQ ID NO: 1645; SEQ ID NO: 1646; SEQ ID NO: 1647; SEQ ID NO: 1648; SEQ ID NO: 1649; SEQ ID NO: 1650; SEQ ID NO: 1651; SEQ ID NO: 1652; SEQ ID NO: 1653; SEQ ID NO: 1654; SEQ ID NO: 1655; SEQ ID NO: 1656; SEQ ID NO: 1657; SEQ ID NO: 1658; SEQ ID NO: 1659; SEQ ID NO: 1660; SEQ ID NO: 1661; SEQ ID NO: 1662; SEQ ID NO: 1663; SEQ ID NO: 1664; SEQ ID NO: 1665; SEQ ID NO: 1666; SEQ ID NO: 1667; SEQ ID NO: 1668; SEQ ID NO: 1669; SEQ ID NO: 1670; SEQ ID NO: 1671; SEQ ID NO: 1672; SEQ ID NO: 1673; SEQ ID NO: 1674; SEQ ID NO: 1675; SEQ ID NO: 1676; SEQ ID NO: 1677; SEQ ID NO: 1678; SEQ ID NO: 1679; SEQ ID NO: 1680; SEQ ID NO: 1681; SEQ ID NO: 1682; SEQ ID NO: 1683; SEQ ID NO: 1684; SEQ ID NO: 1685; SEQ ID NO: 1686; SEQ ID NO: 1687; SEQ ID NO: 1688; SEQ ID NO: 1689; SEQ ID NO: 1690; SEQ ID NO: 1691; SEQ ID NO: 1692; SEQ ID NO: 1693; SEQ ID NO: 1694; SEQ ID NO: 1695; SEQ ID NO: 1696; SEQ ID NO: 1697; SEQ ID NO: 1698; SEQ ID NO: 1699; SEQ ID NO: 1700; SEQ ID NO: 1701; SEQ ID NO: 1702; SEQ ID NO: 1703; SEQ ID NO: 1704; SEQ ID NO: 1705; SEQ ID NO: 1706; SEQ ID NO: 1707; SEQ ID NO: 1708; SEQ ID NO: 1709; SEQ ID NO: 1710; SEQ ID NO: 1711; SEQ ID NO: 1712; SEQ ID NO: 1713; SEQ ID NO: 1714; SEQ ID NO: 1715; SEQ ID NO: 1716; SEQ ID NO: 1717; SEQ ID NO: 1718; SEQ ID NO: 1719; SEQ ID NO: 1720; SEQ ID NO: 1721; SEQ ID NO: 1722; SEQ ID NO: 1723; SEQ ID NO: 1724; SEQ ID NO: 1725; SEQ ID NO: 1726; SEQ ID NO: 1727; SEQ ID NO: 1728; SEQ ID NO: 1729; SEQ ID NO: 1730; SEQ ID NO: 1731; SEQ ID NO: 1732; SEQ ID NO: 1733; SEQ ID NO: 1734; SEQ ID NO: 1735; SEQ ID NO: 1736; SEQ ID NO: 1737; SEQ ID NO: 1738; SEQ ID NO: 1739; SEQ ID NO: 1740; SEQ ID NO: 1741; SEQ ID NO: 1742; SEQ ID NO: 1743; SEQ ID NO: 1744; SEQ ID NO: 1745; SEQ ID NO: 1746; SEQ ID NO: 1747; SEQ ID NO: 1748; SEQ ID NO: 1749; SEQ ID NO: 1750; SEQ ID NO: 1751; SEQ ID NO: 1752; SEQ ID NO: 1753; SEQ ID NO: 1754; SEQ ID NO: 1755; SEQ ID NO: 1756; SEQ ID NO: 1757; SEQ ID NO: 1758; SEQ ID NO: 1759; SEQ ID NO: 1760; SEQ ID NO: 1761; SEQ ID NO: 1762; SEQ ID NO: 1763; SEQ ID NO: 1764; SEQ ID NO: 1765; SEQ ID NO: 1766; SEQ ID NO: 1767; SEQ ID NO: 1768; SEQ ID NO: 1769; SEQ ID NO: 1770; SEQ ID NO: 1771; SEQ ID NO: 1772; SEQ ID NO: 1773; SEQ ID NO: 1774; SEQ ID NO: 1775; SEQ ID NO: 1776; SEQ ID NO: 1777; SEQ ID NO: 1778; SEQ ID NO: 1779; SEQ ID NO: 1780; SEQ ID NO: 1781; SEQ ID NO: 1782; SEQ ID NO: 1783; SEQ ID NO: 1784; SEQ ID NO: 1785; SEQ ID NO: 1786; SEQ ID NO: 1787; SEQ ID NO: 1788; SEQ ID NO: 1789; SEQ ID NO: 1790; SEQ ID NO: 1791; SEQ ID NO: 1792; SEQ ID NO: 1793; SEQ ID NO: 1794; SEQ ID NO: 1795; SEQ ID NO: 1796; SEQ ID NO: 1797; SEQ ID NO: 1798; SEQ ID NO: 1799; SEQ ID NO: 1800; SEQ ID NO: 1801; SEQ ID NO: 1802; SEQ ID NO: 1803; SEQ ID NO: 1804; SEQ ID NO: 1805; SEQ ID NO: 1806; SEQ ID NO: 1807; SEQ ID NO: 1808; SEQ ID NO: 1809; SEQ ID NO: 1810; SEQ ID NO: 1811; SEQ ID NO: 1812; SEQ ID NO: 1813; SEQ ID NO: 1814; SEQ ID NO: 1815; SEQ ID NO: 1816; SEQ ID NO: 1817; SEQ ID NO: 1818; SEQ ID NO: 1819; SEQ ID NO: 1820; SEQ ID NO: 1821; SEQ ID NO: 1822; SEQ ID NO: 1823; SEQ ID NO: 1824; SEQ ID NO: 1825; SEQ ID NO: 1826; SEQ ID NO: 1827; SEQ ID NO: 1828; SEQ ID NO: 1829; SEQ ID NO: 1830; SEQ ID NO: 1831; SEQ ID NO: 1832; SEQ ID NO: 1833; SEQ ID NO: 1834; SEQ ID NO: 1835; SEQ ID NO: 1836; SEQ ID NO: 1837; SEQ ID NO: 1838; SEQ ID NO: 1839; SEQ ID NO: 1840; SEQ ID NO: 1841; SEQ ID NO: 1842; SEQ ID NO: 1843; SEQ ID NO: 1844; SEQ ID NO: 1845; SEQ ID NO: 1846; SEQ ID NO: 1847; SEQ ID NO: 1848; SEQ ID NO: 1849; SEQ ID NO: 1850; SEQ ID NO: 1851; SEQ ID NO: 1852; SEQ ID NO: 1853; SEQ ID NO: 1854; SEQ ID NO: 1855; SEQ ID NO: 1856; SEQ ID NO: 1857; SEQ ID NO: 1858; SEQ ID NO: 1859; SEQ ID NO: 1860; SEQ ID NO: 1861; SEQ ID NO: 1862; SEQ ID NO: 1863; SEQ ID NO: 1864; SEQ ID NO: 1865; SEQ ID NO: 1866; SEQ ID NO: 1867; SEQ ID NO: 1868; SEQ ID NO: 1869; SEQ ID NO: 1870; SEQ ID NO: 1871; SEQ ID NO: 1872; SEQ ID NO: 1873; SEQ ID NO: 1874; SEQ ID NO: 1875; SEQ ID NO: 1876; SEQ ID NO: 1877; SEQ ID NO: 1878; SEQ ID NO: 1879; SEQ ID NO: 1880; SEQ ID NO: 1881; SEQ ID NO: 1882; SEQ ID NO: 1883; SEQ ID NO: 1884; SEQ ID NO: 1885; SEQ ID NO: 1886; SEQ ID NO: 1887; SEQ ID NO: 1888; SEQ ID NO: 1889; SEQ ID NO: 1890; SEQ ID NO: 1891; SEQ ID NO: 1892; SEQ ID NO: 1893; SEQ ID NO: 1894; SEQ ID NO: 1895; SEQ ID NO: 1896; SEQ ID NO: 1897; SEQ ID NO: 1898; SEQ ID NO: 1899; SEQ ID NO: 1900; SEQ ID NO: 1901; SEQ ID NO: 1902; SEQ ID NO: 1903; SEQ ID NO: 1904; SEQ ID NO: 1905; SEQ ID NO: 1906; SEQ ID NO: 1907; SEQ ID NO: 1908; SEQ ID NO: 1909; SEQ ID NO: 1910; SEQ ID NO: 1911; SEQ ID NO: 1912; SEQ ID NO: 1913; SEQ ID NO: 1914; SEQ ID NO: 1915; SEQ ID NO: 1916; SEQ ID NO: 1917; SEQ ID NO: 1918; SEQ ID NO: 1919; SEQ ID NO: 1920; SEQ ID NO: 1921; SEQ ID NO: 1922; SEQ ID NO: 1923; SEQ ID NO: 1924; SEQ ID NO: 1925; SEQ ID NO: 1926; SEQ ID NO: 1927; SEQ ID NO: 1928; SEQ ID NO: 1929; SEQ ID NO: 1930; SEQ ID NO: 1931; SEQ ID NO: 1932; SEQ ID NO: 1933; SEQ ID NO: 1934; SEQ ID NO: 1935; SEQ ID NO: 1936; SEQ ID NO: 1937; SEQ ID NO: 1938; SEQ ID NO: 1939; SEQ ID NO: 1940; SEQ ID NO: 1941; SEQ ID NO: 1942; SEQ ID NO: 1943; SEQ ID NO: 1944; SEQ ID NO: 1945; SEQ ID NO: 1946; SEQ ID NO: 1947; SEQ ID NO: 1948; SEQ ID NO: 1949; SEQ ID NO: 1950; SEQ ID NO: 1951; SEQ ID NO: 1952; SEQ ID NO: 1953; SEQ ID NO: 1954; SEQ ID NO: 1955; SEQ ID NO: 1956; SEQ ID NO: 1957; SEQ ID NO: 1958; SEQ ID NO: 1959; SEQ ID NO: 1960; SEQ ID NO: 1961; SEQ ID NO: 1962; SEQ ID NO: 1963; SEQ ID NO: 1964; SEQ ID NO: 1965; SEQ ID NO: 1966; SEQ ID NO: 1967; SEQ ID NO: 1968; SEQ ID NO: 1969; SEQ ID NO: 1970; SEQ ID NO: 1971; SEQ ID NO: 1972; SEQ ID NO: 1973; SEQ ID NO: 1974; SEQ ID NO: 1975; SEQ ID NO: 1976; SEQ ID NO: 1977; SEQ ID NO: 1978; SEQ ID NO: 1979; SEQ ID NO: 1980; SEQ ID NO: 1981; SEQ ID NO: 1982; SEQ ID NO: 1983; SEQ ID NO: 1984; SEQ ID NO: 1985; SEQ ID NO: 1986; SEQ ID NO: 1987; SEQ ID NO: 1988; SEQ ID NO: 1989; SEQ ID NO: 1990; SEQ ID NO: 1991; SEQ ID NO: 1992; SEQ ID NO: 1993; SEQ ID NO: 1994; SEQ ID NO: 1995; SEQ ID NO: 1996; SEQ ID NO: 1997; SEQ ID NO: 1998; SEQ ID NO: 1999; SEQ ID NO: 2000; SEQ ID NO: 2001; SEQ ID NO: 2002; SEQ ID NO: 2003; SEQ ID NO: 2004; SEQ ID NO: 2005; SEQ ID NO: 2006; SEQ ID NO: 2007; SEQ ID NO: 2008; SEQ ID NO: 2009; SEQ ID NO: 2010; SEQ ID NO: 2011; SEQ ID NO: 2012; SEQ ID NO: 2013; SEQ ID NO: 2014; SEQ ID NO: 2015; SEQ ID NO: 2016; SEQ ID NO: 2017; SEQ ID NO: 2018; SEQ ID NO: 2019; SEQ ID NO: 2020; SEQ ID NO: 2021; SEQ ID NO: 2022; SEQ ID NO: 2023; SEQ ID NO: 2024; SEQ ID NO: 2025; SEQ ID NO: 2026; SEQ ID NO: 2027; SEQ ID NO: 2028; SEQ ID NO: 2029; SEQ ID NO: 2030; SEQ ID NO: 2031; SEQ ID NO: 2032; SEQ ID NO: 2033; SEQ ID NO: 2034; SEQ ID NO: 2035; SEQ ID NO: 2036; SEQ ID NO: 2037; SEQ ID NO: 2038; SEQ ID NO: 2039; SEQ ID NO: 2040; SEQ ID NO: 2041; SEQ ID NO: 2042; SEQ ID NO: 2043; SEQ ID NO: 2044; SEQ ID NO: 2045; SEQ ID NO: 2046; SEQ ID NO: 2047; SEQ ID NO: 2048; SEQ ID NO: 2049; SEQ ID NO: 2050; SEQ ID NO: 2051; SEQ ID NO: 2052; SEQ ID NO: 2053; SEQ ID NO: 2054; SEQ ID NO: 2055; SEQ ID NO: 2056; SEQ ID NO: 2057; SEQ ID NO: 2058; SEQ ID NO: 2059; SEQ ID NO: 2060; SEQ ID NO: 2061; SEQ ID NO: 2062; SEQ ID NO: 2063; SEQ ID NO: 2064; SEQ ID NO: 2065; SEQ ID NO: 2066; SEQ ID NO: 2067; SEQ ID NO: 2068; SEQ ID NO: 2069; SEQ ID NO: 2070; SEQ ID NO: 2071; SEQ ID NO: 2072; SEQ ID NO: 2073; SEQ ID NO: 2074; SEQ ID NO: 2075; SEQ ID NO: 2076; SEQ ID NO: 2077; SEQ ID NO: 2078; SEQ ID NO: 2079; SEQ ID NO: 2080; SEQ ID NO: 2081; SEQ ID NO: 2082; SEQ ID NO: 2083; SEQ ID NO: 2084; SEQ ID NO: 2085; SEQ ID NO: 2086; SEQ ID NO: 2087; SEQ ID NO: 2088; SEQ ID NO: 2089; SEQ ID NO: 2090; SEQ ID NO: 2091; SEQ ID NO: 2092; SEQ ID NO: 2093; SEQ ID NO: 2094; SEQ ID NO: 2095; SEQ ID NO: 2096; SEQ ID NO: 2097; SEQ ID NO: 2098; SEQ ID NO: 2099; SEQ ID NO: 2100; SEQ ID NO: 2101; SEQ ID NO: 2102; SEQ ID NO: 2103; SEQ ID NO: 2104; SEQ ID NO: 2105; SEQ ID NO: 2106; SEQ ID NO: 2107; SEQ ID NO: 2108; SEQ ID NO: 2109; SEQ ID NO: 2110; SEQ ID NO: 2111; SEQ ID NO: 2112; SEQ ID NO: 2113; SEQ ID NO: 2114; SEQ ID NO: 2115; SEQ ID NO: 2116; SEQ ID NO: 2117; SEQ ID NO: 2118; SEQ ID NO: 2119; SEQ ID NO: 2120; SEQ ID NO: 2121; SEQ ID NO: 2122; SEQ ID NO: 2123; SEQ ID NO: 2124; SEQ ID NO: 2125; SEQ ID NO: 2126; SEQ ID NO: 2127; SEQ ID NO: 2128; SEQ ID NO: 2129; SEQ ID NO: 2130; SEQ ID NO: 2131; SEQ ID NO: 2132; SEQ ID NO: 2133; SEQ ID NO: 2134; SEQ ID NO: 2135; SEQ ID NO: 2136; SEQ ID NO: 2137; SEQ ID NO: 2138; SEQ ID NO: 2139; SEQ ID NO: 2140; SEQ ID NO: 2141; SEQ ID NO: 2142; SEQ ID NO: 2143; SEQ ID NO: 2144; SEQ ID NO: 2145; SEQ ID NO: 2146; SEQ ID NO: 2147; SEQ ID NO: 2148; SEQ ID NO: 2149; SEQ ID NO: 2150; SEQ ID NO: 2151; SEQ ID NO: 2152; SEQ ID NO: 2153; SEQ ID NO: 2154; SEQ ID NO: 2155; SEQ ID NO: 2156; SEQ ID NO: 2157; SEQ ID NO: 2158; SEQ ID NO: 2159; SEQ ID NO: 2160; SEQ ID NO: 2161; SEQ ID NO: 2162; SEQ ID NO: 2163; SEQ ID NO: 2164; SEQ ID NO: 2165; SEQ ID NO: 2166; SEQ ID NO: 2167; SEQ ID NO: 2168; SEQ ID NO: 2169; SEQ ID NO: 2170; SEQ ID NO: 2171; SEQ ID NO: 2172; SEQ ID NO: 2173; SEQ ID NO: 2174; SEQ ID NO: 2175; SEQ ID NO: 2176; SEQ ID NO: 2177; SEQ ID NO: 2178; SEQ ID NO: 2179; SEQ ID NO: 2180; SEQ ID NO: 2181; SEQ ID NO: 2182; SEQ ID NO: 2183; SEQ ID NO: 2184; SEQ ID NO: 2185; SEQ ID NO: 2186; SEQ ID NO: 2187; SEQ ID NO: 2188; SEQ ID NO: 2189; SEQ ID NO: 2190; SEQ ID NO: 2191; SEQ ID NO: 2192; SEQ ID NO: 2193; SEQ ID NO: 2194; SEQ ID NO: 2195; SEQ ID NO: 2196; SEQ ID NO: 2197; SEQ ID NO: 2198; SEQ ID NO: 2199; SEQ ID NO: 2200; SEQ ID NO: 2201; SEQ ID NO: 2202; SEQ ID NO: 2203; SEQ ID NO: 2204; SEQ ID NO: 2205; SEQ ID NO: 2206; SEQ ID NO: 2207; SEQ ID NO: 2208; SEQ ID NO: 2209; SEQ ID NO: 2210; SEQ ID NO: 2211; SEQ ID NO: 2212; SEQ ID NO: 2213; SEQ ID NO: 2214; SEQ ID NO: 2215; SEQ ID NO: 2216; SEQ ID NO: 2217; SEQ ID NO: 2218; SEQ ID NO: 2219; SEQ ID NO: 2220; SEQ ID NO: 2221; SEQ ID NO: 2222; SEQ ID NO: 2223; SEQ ID NO: 2224; SEQ ID NO: 2225; SEQ ID NO: 2226; SEQ ID NO: 2227; SEQ ID NO: 2228; SEQ ID NO: 2229; SEQ ID NO: 2230; SEQ ID NO: 2231; SEQ ID NO: 2232; SEQ ID NO: 2233; SEQ ID NO: 2234; SEQ ID NO: 2235; SEQ ID NO: 2236; SEQ ID NO: 2237; SEQ ID NO: 2238; SEQ ID NO: 2239; SEQ ID NO: 2240; SEQ ID NO: 2241; SEQ ID NO: 2242; SEQ ID NO: 2243; SEQ ID NO: 2244; SEQ ID NO: 2245; SEQ ID NO: 2246; SEQ ID NO: 2247; SEQ ID NO: 2248; SEQ ID NO: 2249; SEQ ID NO: 2250; SEQ ID NO: 2251; SEQ ID NO: 2252; SEQ ID NO: 2253; SEQ ID NO: 2254; SEQ ID NO: 2255; SEQ ID NO: 2256; SEQ ID NO: 2257; SEQ ID NO: 2258; SEQ ID NO: 2259; SEQ ID NO: 2260; SEQ ID NO: 2261; SEQ ID NO: 2262; SEQ ID NO: 2263; SEQ ID NO: 2264; SEQ ID NO: 2265; SEQ ID NO: 2266; SEQ ID NO: 2267; SEQ ID NO: 2268; SEQ ID NO: 2269; SEQ ID NO: 2270; SEQ ID NO: 2271; SEQ ID NO: 2272; SEQ ID NO: 2273; SEQ ID NO: 2274; SEQ ID NO: 2275; SEQ ID NO: 2276; SEQ ID NO: 2277; SEQ ID NO: 2278; SEQ ID NO: 2279; SEQ ID NO: 2280; SEQ ID NO: 2281; SEQ ID NO: 2282; SEQ ID NO: 2283; SEQ ID NO: 2284; SEQ ID NO: 2285; SEQ ID NO: 2286; SEQ ID NO: 2287; SEQ ID NO: 2288; SEQ ID NO: 2289; SEQ ID NO: 2290; SEQ ID NO: 2291; SEQ ID NO: 2292; SEQ ID NO: 2293; SEQ ID NO: 2294; SEQ ID NO: 2295; SEQ ID NO: 2296; SEQ ID NO: 2297; SEQ ID NO: 2298; SEQ ID NO: 2299; SEQ ID NO: 2300; SEQ ID NO: 2301; SEQ ID NO: 2302; SEQ ID NO: 2303; SEQ ID NO: 2304; SEQ ID NO: 2305; SEQ ID NO: 2306; SEQ ID NO: 2307; SEQ ID NO: 2308; SEQ ID NO: 2309; SEQ ID NO: 2310; SEQ ID NO: 2311; SEQ ID NO: 2312; SEQ ID NO: 2313; SEQ ID NO: 2314; SEQ ID NO: 2315; SEQ ID NO: 2316; SEQ ID NO: 2317; SEQ ID NO: 2318; SEQ ID NO: 2319; SEQ ID NO: 2320; SEQ ID NO: 2321; SEQ ID NO: 2322; SEQ ID NO: 2323; SEQ ID NO: 2324; SEQ ID NO: 2325; SEQ ID NO: 2326; SEQ ID NO:2327; SEQ ID NO: 2328; SEQ ID NO: 2329; SEQ ID NO: 2330; SEQ ID NO: 2331; SEQ ID NO: 2332; SEQ ID NO: 2333; SEQ ID NO: 2334; SEQ ID NO: 2335; SEQ ID NO: 2336; SEQ ID NO: 2337; SEQ ID NO: 2338; SEQ ID NO: 2339; SEQ ID NO: 2340; SEQ ID NO: 2341; SEQ ID NO: 2342; SEQ ID NO: 2343; SEQ ID NO: 2344; SEQ ID NO: 2345; SEQ ID NO: 2346; SEQ ID NO: 2347; SEQ ID NO: 2348; SEQ ID NO: 2349; SEQ ID NO: 2350; SEQ ID NO: 2351; SEQ ID NO: 2352; SEQ ID NO: 2353; SEQ ID NO: 2354; SEQ ID NO: 2355; SEQ ID NO: 2356; SEQ ID NO: 2357; SEQ ID NO: 2358; SEQ ID NO: 2359; SEQ ID NO: 2360; SEQ ID NO: 2361; SEQ ID NO: 2362; SEQ ID NO: 2363; SEQ ID NO: 2364; SEQ ID NO: 2365; SEQ ID NO: 2366; SEQ ID NO: 2367; SEQ ID NO: 2368; SEQ ID NO: 2369; SEQ ID NO: 2370; SEQ ID NO: 2371; SEQ ID NO: 2372; SEQ ID NO: 2373; SEQ ID NO: 2374; SEQ ID NO: 2375; SEQ ID NO: 2376; SEQ ID NO: 2377; SEQ ID NO: 2378; SEQ ID NO: 2379; SEQ ID NO: 2380; SEQ ID NO: 2381; SEQ ID NO: 2382; SEQ ID NO: 2383; SEQ ID NO: 2384; SEQ ID NO: 2385; SEQ ID NO: 2386; SEQ ID NO: 2387; SEQ ID NO: 2388; SEQ ID NO: 2389; SEQ ID NO: 2390; SEQ ID NO: 2391; SEQ ID NO: 2392; SEQ ID NO: 2393; SEQ ID NO: 2394; SEQ ID NO: 2395; SEQ ID NO: 2396; SEQ ID NO: 2397; SEQ ID NO: 2398; SEQ ID NO: 2399; SEQ ID NO: 2400; SEQ ID NO: 2401; SEQ ID NO: 2402; SEQ ID NO: 2403; SEQ ID NO: 2404; SEQ ID NO: 2405; SEQ ID NO: 2406; SEQ ID NO: 2407; SEQ ID NO: 2408; SEQ ID NO: 2409; SEQ ID NO:2410; SEQ ID NO:2411; SEQ ID NO:2412; SEQ ID NO:2413; SEQ ID NO: 2414; SEQ ID NO: 2415; SEQ ID NO: 2416; SEQ ID NO: 2417; SEQ ID NO: 2418; SEQ ID NO: 2419; SEQ ID NO: 2420; SEQ ID NO: 2421; SEQ ID NO: 2422; SEQ ID NO: 2423; SEQ ID NO: 2424; SEQ ID NO: 2425; SEQ ID NO: 2426; SEQ ID NO: 2427; SEQ ID NO: 2428; SEQ ID NO: 2429; SEQ ID NO: 2430; SEQ ID NO: 2431; SEQ ID NO: 2432; SEQ ID NO: 2433; SEQ ID NO: 2434; SEQ ID NO: 2435; SEQ ID NO: 2436; SEQ ID NO: 2437; SEQ ID NO: 2438; SEQ ID NO: 2439; SEQ ID NO: 2440; SEQ ID NO: 2441; SEQ ID NO: 2442; SEQ ID NO: 2443; SEQ ID NO: 2444; SEQ ID NO: 2445; SEQ ID NO: 2446; SEQ ID NO: 2447; SEQ ID NO: 2448; SEQ ID NO: 2449; SEQ ID NO: 2450; SEQ ID NO: 2451; SEQ ID NO: 2452; SEQ ID NO: 2453; SEQ ID NO: 2454; SEQ ID NO: 2455; SEQ ID NO: 2456; SEQ ID NO: 2457; SEQ ID NO: 2458; SEQ ID NO: 2459; SEQ ID NO: 2460; SEQ ID NO: 2461; SEQ ID NO: 2462; SEQ ID NO: 2463; SEQ ID NO: 2464; SEQ ID NO: 2465; SEQ ID NO: 2466; SEQ ID NO: 2467; SEQ ID NO: 2468; SEQ ID NO: 2469; SEQ ID NO: 2470; SEQ ID NO: 2471; SEQ ID NO: 2472; SEQ ID NO: 2473; SEQ ID NO: 2474; SEQ ID NO: 2475; SEQ ID NO: 2476; SEQ ID NO: 2477; SEQ ID NO: 2478; SEQ ID NO: 2479; SEQ ID NO: 2480; SEQ ID NO: 2481; SEQ ID NO: 2482; SEQ ID NO: 2483; SEQ ID NO: 2484; SEQ ID NO: 2485; SEQ ID NO: 2486; SEQ ID NO: 2487; SEQ ID NO: 2488; SEQ ID NO: 2489; SEQ ID NO: 2490; SEQ ID NO: 2491; SEQ ID NO: 2492; SEQ ID NO: 2493; SEQ ID NO: 2494; SEQ ID NO: 2495; SEQ ID NO: 2496; SEQ ID NO: 2497; SEQ ID NO: 2498; SEQ ID NO: 2499; SEQ ID NO: 2500; SEQ ID NO: 2501; SEQ ID NO: 2502; SEQ ID NO: 2503; SEQ ID NO: 2504; SEQ ID NO: 2505; SEQ ID NO: 2506; SEQ ID NO: 2507; SEQ ID NO: 2508; SEQ ID NO: 2509; SEQ ID NO: 2510; SEQ ID NO: 2511; SEQ ID NO: 2512; SEQ ID NO: 2513; SEQ ID NO: 2514; SEQ ID NO: 2515; SEQ ID NO: 2516; SEQ ID NO: 2517; SEQ ID NO: 2518; SEQ ID NO: 2519; SEQ ID NO: 2520; SEQ ID NO: 2521; SEQ ID NO: 2522; SEQ ID NO: 2523; SEQ ID NO: 2524; SEQ ID NO: 2525; SEQ ID NO: 2526; SEQ ID NO: 2527; SEQ ID NO: 2528; SEQ ID NO: 2529; SEQ ID NO: 2530; SEQ ID NO: 2531; SEQ ID NO: 2532; SEQ ID NO: 2533; SEQ ID NO: 2534; SEQ ID NO: 2535; SEQ ID NO: 2536; SEQ ID NO: 2537; SEQ ID NO: 2538; SEQ ID NO: 2539; SEQ ID NO: 2540; SEQ ID NO: 2541; SEQ ID NO: 2542; SEQ ID NO: 2543; SEQ ID NO: 2544; SEQ ID NO: 2545; SEQ ID NO: 2546; SEQ ID NO: 2547; SEQ ID NO: 2548; SEQ ID NO: 2549; SEQ ID NO: 2550; SEQ ID NO: 2551; SEQ ID NO: 2552; SEQ ID NO: 2553; SEQ ID NO: 2554; SEQ ID NO: 2555; SEQ ID NO: 2556; SEQ ID NO: 2557; SEQ ID NO: 2558; SEQ ID NO: 2559; SEQ ID NO: 2560; SEQ ID NO: 2561; SEQ ID NO: 2562; SEQ ID NO: 2563; SEQ ID NO: 2564; SEQ ID NO: 2565; SEQ ID NO: 2566; SEQ ID NO: 2567; SEQ ID NO: 2568; SEQ ID NO: 2569; SEQ ID NO: 2570; SEQ ID NO: 2571; SEQ ID NO: 2572; SEQ ID NO: 2573; SEQ ID NO: 2574; SEQ ID NO: 2575; SEQ ID NO: 2576; SEQ ID NO: 2577; SEQ ID NO: 2578; SEQ ID NO: 2579; SEQ ID NO: 2580; SEQ ID NO: 2581; SEQ ID NO: 2582; SEQ ID NO: 2583; SEQ ID NO: 2584; SEQ ID NO: 2585; SEQ ID NO: 2586; SEQ ID NO: 2587; SEQ ID NO: 2588; SEQ ID NO: 2589; SEQ ID NO: 2590; SEQ ID NO: 2591; SEQ ID NO: 2592; SEQ ID NO: 2593; SEQ ID NO: 2594; SEQ ID NO: 2595; SEQ ID NO: 2596; SEQ ID NO: 2597; SEQ ID NO: 2598; SEQ ID NO: 2599; SEQ ID NO: 2600; SEQ ID NO: 2601; SEQ ID NO: 2602; SEQ ID NO: 2603; SEQ ID NO: 2604; SEQ ID NO: 2605; SEQ ID NO: 2606; SEQ ID NO: 2607; SEQ ID NO: 2608; SEQ ID NO: 2609; SEQ ID NO: 2610; SEQ ID NO: 2611; SEQ ID NO: 2612; SEQ ID NO: 2613; SEQ ID NO: 2614; SEQ ID NO: 2615; SEQ ID NO: 2616; SEQ ID NO: 2617; SEQ ID NO: 2618; SEQ ID NO: 2619; SEQ ID NO: 2620; SEQ ID NO: 2621; SEQ ID NO: 2622; SEQ ID NO: 2623; SEQ ID NO: 2624; SEQ ID NO: 2625; SEQ ID NO: 2626; SEQ ID NO: 2627; SEQ ID NO: 2628; SEQ ID NO: 2629; SEQ ID NO: 2630; SEQ ID NO: 2631; SEQ ID NO: 2632; SEQ ID NO: 2633; SEQ ID NO: 2634; SEQ ID NO: 2635; SEQ ID NO: 2636; SEQ ID NO: 2637; SEQ ID NO: 2638; SEQ ID NO: 2639; SEQ ID NO: 2640; SEQ ID NO: 2641; SEQ ID NO: 2642; SEQ ID NO: 2643; SEQ ID NO: 2644; SEQ ID NO: 2645; SEQ ID NO: 2646; SEQ ID NO: 2647; SEQ ID NO: 2648; SEQ ID NO: 2649; SEQ ID NO: 2650; SEQ ID NO: 2651; SEQ ID NO: 2652; SEQ ID NO: 2653; SEQ ID NO: 2654; SEQ ID NO: 2655; SEQ ID NO: 2656; SEQ ID NO: 2657; SEQ ID NO: 2658; SEQ ID NO: 2659; SEQ ID NO: 2660; SEQ ID NO: 2661; SEQ ID NO: 2662; SEQ ID NO: 2663; SEQ ID NO: 2664; SEQ ID NO: 2665; SEQ ID NO: 2666; SEQ ID NO: 2667; SEQ ID NO: 2668; SEQ ID NO: 2669; SEQ ID NO: 2670; SEQ ID NO: 2671; SEQ ID NO: 2672; SEQ ID NO: 2673; SEQ ID NO: 2674; SEQ ID NO: 2675; SEQ ID NO: 2676; SEQ ID NO: 2677; SEQ ID NO: 2678; SEQ ID NO: 2679; SEQ ID NO: 2680; SEQ ID NO: 2681; SEQ ID NO: 2682; SEQ ID NO: 2683; SEQ ID NO: 2684; SEQ ID NO: 2685; SEQ ID NO: 2686; SEQ ID NO: 2687; SEQ ID NO: 2688; SEQ ID NO: 2689; SEQ ID NO: 2690; SEQ ID NO: 2691; SEQ ID NO: 2692; SEQ ID NO: 2693; SEQ ID NO: 2694; SEQ ID NO: 2695; SEQ ID NO: 2696; SEQ ID NO: 2697; SEQ ID NO: 2698; SEQ ID NO: 2699; SEQ ID NO: 2700; SEQ ID NO: 2701; SEQ ID NO: 2702; SEQ ID NO: 2703; SEQ ID NO: 2704; SEQ ID NO: 2705; SEQ ID NO: 2706; SEQ ID NO: 2707; SEQ ID NO: 2708; SEQ ID NO: 2709; SEQ ID NO: 2710; SEQ ID NO: 2711; SEQ ID NO: 2712; SEQ ID NO: 2713; SEQ ID NO: 2714; SEQ ID NO: 2715; SEQ ID NO: 2716; SEQ ID NO: 2717; SEQ ID NO: 2718; SEQ ID NO: 2719; SEQ ID NO: 2720; SEQ ID NO: 2721; SEQ ID NO: 2722; SEQ ID NO: 2723; SEQ ID NO: 2724; SEQ ID NO: 2725; SEQ ID NO: 2726; SEQ ID NO: 2727; SEQ ID NO: 2728; SEQ ID NO: 2729; SEQ ID NO: 2730; SEQ ID NO: 2731; SEQ ID NO: 2732; SEQ ID NO: 2733; SEQ ID NO: 2734; SEQ ID NO: 2735; SEQ ID NO: 2736; SEQ ID NO: 2737; SEQ ID NO: 2738; SEQ ID NO: 2739; SEQ ID NO: 2740; SEQ ID NO: 2741; SEQ ID NO: 2742; SEQ ID NO: 2743; SEQ ID NO: 2744; SEQ ID NO: 2745; SEQ ID NO: 2746; SEQ ID NO: 2747; SEQ ID NO: 2748; SEQ ID NO: 2749; SEQ ID NO: 2750; SEQ ID NO: 2751; SEQ ID NO: 2752; SEQ ID NO: 2753; SEQ ID NO: 2754; SEQ ID NO: 2755; SEQ ID NO: 2756; SEQ ID NO: 2757; SEQ ID NO: 2758; SEQ ID NO: 2759; SEQ ID NO: 2760; SEQ ID NO: 2761; SEQ ID NO: 2762; SEQ ID NO: 2763; SEQ ID NO: 2764; SEQ ID NO: 2765; SEQ ID NO: 2766; SEQ ID NO: 2767; SEQ ID NO: 2768; SEQ ID NO: 2769; SEQ ID NO: 2770; SEQ ID NO: 2771; SEQ ID NO: 2772; SEQ ID NO: 2773; SEQ ID NO: 2774; SEQ ID NO: 2775; SEQ ID NO: 2776; SEQ ID NO: 2777; SEQ ID NO: 2778; SEQ ID NO: 2779; SEQ ID NO: 2780; SEQ ID NO: 2781; SEQ ID NO: 2782; SEQ ID NO: 2783; SEQ ID NO: 2784; SEQ ID NO: 2785; SEQ ID NO: 2786; SEQ ID NO: 2787; SEQ ID NO: 2788; SEQ ID NO: 2789; SEQ ID NO: 2790; SEQ ID NO: 2791; SEQ ID NO: 2792; SEQ ID NO: 2793; SEQ ID NO: 2794; SEQ ID NO: 2795; SEQ ID NO: 2796; SEQ ID NO: 2797; SEQ ID NO: 2798; SEQ ID NO: 2799; SEQ ID NO: 2800; SEQ ID NO: 2801; SEQ ID NO: 2802; SEQ ID NO: 2803; SEQ ID NO: 2804; SEQ ID NO: 2805; SEQ ID NO: 2806; SEQ ID NO: 2807; SEQ ID NO: 2808; SEQ ID NO: 2809; SEQ ID NO: 2810; SEQ ID NO: 2811; SEQ ID NO: 2812; SEQ ID NO: 2813; SEQ ID NO: 2814; SEQ ID NO: 2815; SEQ ID NO: 2816; SEQ ID NO: 2817; SEQ ID NO: 2818; SEQ ID NO: 2819; SEQ ID NO: 2820; SEQ ID NO: 2821; SEQ ID NO: 2822; SEQ ID NO: 2823; SEQ ID NO: 2824; SEQ ID NO: 2825; SEQ ID NO: 2826; SEQ ID NO: 2827; SEQ ID NO: 2828; SEQ ID NO: 2829; SEQ ID NO: 2830; SEQ ID NO: 2831; SEQ ID NO: 2832; SEQ ID NO: 2833; SEQ ID NO: 2834; SEQ ID NO: 2835; SEQ ID NO: 2836; SEQ ID NO: 2837; SEQ ID NO: 2838; SEQ ID NO: 2839; SEQ ID NO: 2840; SEQ ID NO: 2841; SEQ ID NO: 2842; SEQ ID NO: 2843; SEQ ID NO: 2844; SEQ ID NO: 2845; SEQ ID NO: 2846; SEQ ID NO: 2847; SEQ ID NO: 2848; SEQ ID NO: 2849; SEQ ID NO: 2850; SEQ ID NO: 2851; SEQ ID NO: 2852; SEQ ID NO: 2853; SEQ ID NO: 2854; SEQ ID NO: 2855; SEQ ID NO: 2856; SEQ ID NO: 2857; SEQ ID NO: 2858; SEQ ID NO: 2859; SEQ ID NO: 2860; SEQ ID NO: 2861; SEQ ID NO: 2862; SEQ ID NO: 2863; SEQ ID NO: 2864; SEQ ID NO: 2865; SEQ ID NO: 2866; SEQ ID NO: 2867; SEQ ID NO: 2868; SEQ ID NO: 2869; SEQ ID NO: 2870; SEQ ID NO: 2871; SEQ ID NO: 2872; SEQ ID NO: 2873; SEQ ID NO: 2874; SEQ ID NO: 2875; SEQ ID NO: 2876; SEQ ID NO: 2877; SEQ ID NO: 2878; SEQ ID NO: 2879; SEQ ID NO: 2880; SEQ ID NO: 2881; SEQ ID NO: 2882; SEQ ID NO: 2883; SEQ ID NO: 2884; SEQ ID NO: 2885; SEQ ID NO: 2886; SEQ ID NO: 2887; SEQ ID NO: 2888; SEQ ID NO: 2889; SEQ ID NO: 2890; SEQ ID NO: 2891; SEQ ID NO: 2892; SEQ ID NO: 2893; SEQ ID NO: 2894; SEQ ID NO: 2895; SEQ ID NO: 2896; SEQ ID NO: 2897; SEQ ID NO: 2898; SEQ ID NO: 2899; SEQ ID NO: 2900; SEQ ID NO: 2901; SEQ ID NO: 2902; SEQ ID NO: 2903; SEQ ID NO: 2904; SEQ ID NO: 2905; SEQ ID NO: 2906; SEQ ID NO: 2907; SEQ ID NO: 2908; SEQ ID NO: 2909; SEQ ID NO: 2910; SEQ ID NO: 2911; SEQ ID NO: 2912; SEQ ID NO: 2913; SEQ ID NO: 2914; SEQ ID NO: 2915; SEQ ID NO: 2916; SEQ ID NO: 2917; SEQ ID NO: 2918; SEQ ID NO: 2919; SEQ ID NO: 2920; SEQ ID NO: 2921; SEQ ID NO: 2922; SEQ ID NO: 2923; SEQ ID NO: 2924; SEQ ID NO: 2925; SEQ ID NO: 2926; SEQ ID NO: 2927; SEQ ID NO: 2928; SEQ ID NO: 2929; SEQ ID NO: 2930; SEQ ID NO: 2931; SEQ ID NO: 2932; SEQ ID NO: 2933; SEQ ID NO: 2934; SEQ ID NO: 2935; SEQ ID NO: 2936; SEQ ID NO: 2937; SEQ ID NO: 2938; SEQ ID NO: 2939; SEQ ID NO: 2940; SEQ ID NO: 2941; SEQ ID NO: 2942; SEQ ID NO: 2943; SEQ ID NO: 2944; SEQ ID NO: 2945; SEQ ID NO: 2946; SEQ ID NO: 2947; SEQ ID NO: 2948; SEQ ID NO: 2949; SEQ ID NO: 2950; SEQ ID NO: 2951; SEQ ID NO: 2952; SEQ ID NO: 2953; SEQ ID NO: 2954; SEQ ID NO: 2955; SEQ ID NO: 2956; SEQ ID NO: 2957; SEQ ID NO: 2958; SEQ ID NO: 2959; SEQ ID NO: 2960; SEQ ID NO: 2961; SEQ ID NO: 2962; SEQ ID NO: 2963; SEQ ID NO: 2964; SEQ ID NO: 2965; SEQ ID NO: 2966; SEQ ID NO: 2967; SEQ ID NO: 2968; SEQ ID NO: 2969; SEQ ID NO: 2970; SEQ ID NO: 2971; SEQ ID NO: 2972; SEQ ID NO: 2973; SEQ ID NO: 2974; SEQ ID NO: 2975; SEQ ID NO: 2976; SEQ ID NO: 2977; SEQ ID NO: 2978; SEQ ID NO: 2979; SEQ ID NO: 2980; SEQ ID NO: 2981; SEQ ID NO: 2982; SEQ ID NO: 2983; SEQ ID NO: 2984; SEQ ID NO: 2985; SEQ ID NO: 2986; SEQ ID NO: 2987; SEQ ID NO: 2988; SEQ ID NO: 2989; SEQ ID NO: 2990; SEQ ID NO: 2991; SEQ ID NO: 2992; SEQ ID NO: 2993; SEQ ID NO: 2994; SEQ ID NO: 2995; SEQ ID NO: 2996; SEQ ID NO: 2997; SEQ ID NO: 2998; SEQ ID NO: 2999; SEQ ID NO: 3000; SEQ ID NO: 3001; SEQ ID NO: 3002; SEQ ID NO: 3003; SEQ ID NO: 3004; SEQ ID NO: 3005; SEQ ID NO: 3006; SEQ ID NO: 3007; SEQ ID NO: 3008; SEQ ID NO: 3009; SEQ ID NO: 3010; SEQ ID NO: 3011; SEQ ID NO: 3012; SEQ ID NO: 3013; SEQ ID NO: 3014; SEQ ID NO: 3015; SEQ ID NO: 3016; SEQ ID NO: 3017; SEQ ID NO: 3018; SEQ ID NO: 3019; SEQ ID NO: 3020; SEQ ID NO: 3021; SEQ ID NO: 3022; SEQ ID NO: 3023; SEQ ID NO: 3024; SEQ ID NO: 3025; SEQ ID NO: 3026; SEQ ID NO: 3027; SEQ ID NO: 3028; SEQ ID NO: 3029; SEQ ID NO: 3030; SEQ ID NO: 3031; SEQ ID NO: 3032; SEQ ID NO: 3033; SEQ ID NO: 3034; SEQ ID NO: 3035; SEQ ID NO: 3036; SEQ ID NO: 3037; SEQ ID NO: 3038; SEQ ID NO: 3039; SEQ ID NO: 3040; SEQ ID NO: 3041; SEQ ID NO: 3042; SEQ ID NO: 3043; SEQ ID NO: 3044; SEQ ID NO: 3045; SEQ ID NO: 3046; SEQ ID NO: 3047; SEQ ID NO: 3048; SEQ ID NO: 3049; SEQ ID NO: 3050; SEQ ID NO: 3051; SEQ ID NO: 3052; SEQ ID NO: 3053; SEQ ID NO: 3054; SEQ ID NO: 3055; SEQ ID NO: 3056; SEQ ID NO: 3057; SEQ ID NO: 3058; SEQ ID NO: 3059; SEQ ID NO: 3060; SEQ ID NO: 3061; SEQ ID NO: 3062; SEQ ID NO: 3063; SEQ ID NO: 3064; SEQ ID NO: 3065; SEQ ID NO: 3066; SEQ ID NO: 3067; SEQ ID NO: 3068; SEQ ID NO: 3069; SEQ ID NO: 3070; SEQ ID NO: 3071; SEQ ID NO: 3072; SEQ ID NO: 3073; SEQ ID NO: 3074; SEQ ID NO: 3075; SEQ ID NO: 3076; SEQ ID NO: 3077; SEQ ID NO: 3078; SEQ ID NO: 3079; SEQ ID NO: 3080; SEQ ID NO: 3081; SEQ ID NO: 3082; SEQ ID NO: 3083; SEQ ID NO: 3084; SEQ ID NO: 3085; SEQ ID NO: 3086; SEQ ID NO: 3087; SEQ ID NO: 3088; SEQ ID NO: 3089; SEQ ID NO: 3090; SEQ ID NO: 3091; SEQ ID NO: 3092; SEQ ID NO: 3093; SEQ ID NO: 3094; SEQ ID NO: 3095; SEQ ID NO: 3096; SEQ ID NO: 3097; SEQ ID NO: 3098; SEQ ID NO: 3099; SEQ ID NO: 3100; SEQ ID NO: 3101; SEQ ID NO: 3102; SEQ ID NO: 3103; SEQ ID NO: 3104; SEQ ID NO: 3105; SEQ ID NO: 3106; SEQ ID NO: 3107; SEQ ID NO: 3108; SEQ ID NO: 3109; SEQ ID NO: 3110; SEQ ID NO: 3111; SEQ ID NO: 3112; SEQ ID NO: 3113; SEQ ID NO: 3114; SEQ ID NO: 3115; SEQ ID NO: 3116; SEQ ID NO: 3117; SEQ ID NO: 3118; SEQ ID NO: 3119; SEQ ID NO: 3120; SEQ ID NO: 3121; SEQ ID NO: 3122; SEQ ID NO: 3123; SEQ ID NO: 3124; SEQ ID NO: 3125; SEQ ID NO: 3126; SEQ ID NO: 3127; SEQ ID NO: 3128; SEQ ID NO: 3129; SEQ ID NO: 3130; SEQ ID NO: 3131; SEQ ID NO: 3132; SEQ ID NO: 3133; SEQ ID NO: 3134; SEQ ID NO: 3135; SEQ ID NO: 3136; SEQ ID NO: 3137; SEQ ID NO: 3138; SEQ ID NO: 3139; SEQ ID NO: 3140; SEQ ID NO: 3141; SEQ ID NO: 3142; SEQ ID NO: 3143; SEQ ID NO: 3144; SEQ ID NO: 3145; SEQ ID NO: 3146; SEQ ID NO: 3147; SEQ ID NO: 3148; SEQ ID NO: 3149; SEQ ID NO: 3150; SEQ ID NO: 3151; SEQ ID NO: 3152; SEQ ID NO: 3153; SEQ ID NO: 3154; SEQ ID NO: 3155; SEQ ID NO: 3156; SEQ ID NO: 3157; SEQ ID NO: 3158; SEQ ID NO: 3159; SEQ ID NO: 3160; SEQ ID NO: 3161; SEQ ID NO: 3162; SEQ ID NO: 3163; SEQ ID NO: 3164; SEQ ID NO: 3165; SEQ ID NO: 3166; SEQ ID NO: 3167; SEQ ID NO: 3168; SEQ ID NO: 3169; SEQ ID NO: 3170; SEQ ID NO: 3171; SEQ ID NO: 3172; SEQ ID NO: 3173; SEQ ID NO: 3174; SEQ ID NO: 3175; SEQ ID NO: 3176; SEQ ID NO: 3177; SEQ ID NO: 3178; SEQ ID NO: 3179; SEQ ID NO: 3180; SEQ ID NO: 3181; SEQ ID NO: 3182; SEQ ID NO: 3183; SEQ ID NO: 3184; SEQ ID NO: 3185; SEQ ID NO: 3186; SEQ ID NO: 3187; SEQ ID NO: 3188; SEQ ID NO: 3189; SEQ ID NO: 3190; SEQ ID NO: 3191; SEQ ID NO: 3192; SEQ ID NO: 3193; SEQ ID NO: 3194; SEQ ID NO: 3195; SEQ ID NO: 3196; SEQ ID NO: 3197; SEQ ID NO: 3198; SEQ ID NO: 3199; SEQ ID NO: 3200; SEQ ID NO: 3201; SEQ ID NO: 3202; SEQ ID NO: 3203; SEQ ID NO: 3204; SEQ ID NO: 3205; SEQ ID NO: 3206; SEQ ID NO: 3207; SEQ ID NO: 3208; SEQ ID NO: 3209; SEQ ID NO: 3210; SEQ ID NO: 3211; SEQ ID NO: 3212; SEQ ID NO: 3213; SEQ ID NO: 3214; SEQ ID NO: 3215; SEQ ID NO: 3216; SEQ ID NO: 3217; SEQ ID NO: 3218; SEQ ID NO: 3219; SEQ ID NO: 3220; SEQ ID NO: 3221; SEQ ID NO: 3222; SEQ ID NO: 3223; SEQ ID NO: 3224; SEQ ID NO: 3225; SEQ ID NO: 3226; SEQ ID NO: 3227; SEQ ID NO: 3228; SEQ ID NO: 3229; SEQ ID NO: 3230; SEQ ID NO: 3231; SEQ ID NO: 3232; SEQ ID NO: 3233; SEQ ID NO: 3234; SEQ ID NO: 3235; SEQ ID NO: 3236; SEQ ID NO: 3237; SEQ ID NO: 3238; SEQ ID NO: 3239; SEQ ID NO: 3240; SEQ ID NO: 3241; SEQ ID NO: 3242; SEQ ID NO: 3243; SEQ ID NO: 3244; SEQ ID NO: 3245; SEQ ID NO: 3246; SEQ ID NO: 3247; SEQ ID NO: 3248; SEQ ID NO: 3249; SEQ ID NO: 3250; SEQ ID NO: 3251; SEQ ID NO: 3252; SEQ ID NO: 3253; SEQ ID NO: 3254; SEQ ID NO: 3255; SEQ ID NO: 3256; SEQ ID NO: 3257; SEQ ID NO: 3258; SEQ ID NO: 3259; SEQ ID NO: 3260; SEQ ID NO: 3261; SEQ ID NO: 3262; SEQ ID NO: 3263; SEQ ID NO: 3264; SEQ ID NO: 3265; SEQ ID NO: 3266; SEQ ID NO: 3267; SEQ ID NO: 3268; SEQ ID NO: 3269; SEQ ID NO: 3270; SEQ ID NO: 3271; SEQ ID NO: 3272; SEQ ID NO: 3273; SEQ ID NO: 3274; SEQ ID NO: 3275; SEQ ID NO: 3276; SEQ ID NO: 3277; SEQ ID NO: 3278; SEQ ID NO: 3279; SEQ ID NO: 3280; SEQ ID NO: 3281; SEQ ID NO: 3282; SEQ ID NO: 3283; SEQ ID NO: 3284; SEQ ID NO: 3285; SEQ ID NO: 3266, SEQ ID NO: 3287; SEQ ID NO: 3288; SEQ ID NO: 3289; SEQ ID NO: 3290; SEQ ID NO: 3291; SEQ ID NO: 3292; SEQ ID NO: 3293; SEQ ID NO: 3294; SEQ ID NO: 3295; SEQ ID NO: 3296; SEQ ID NO: 3297; SEQ ID NO: 3298; SEQ ID NO: 3299; SEQ ID NO: 3300; SEQ ID NO: 3301; SEQ ID NO: 3302; SEQ ID NO: 3303; SEQ ID NO: 3304; SEQ ID NO: 3305; SEQ ID NO: 3306; SEQ ID NO: 3307; SEQ ID NO: 3308; SEQ ID NO: 3309; SEQ ID NO: 3310; SEQ ID NO: 3311; SEQ ID NO: 3312; SEQ ID NO: 3313; SEQ ID NO: 3314; SEQ ID NO: 3315; SEQ ID NO: 3316; SEQ ID NO: 3317; SEQ ID NO:3318; SEQ ID NO: 3319; SEQ ID NO: 3320; SEQ ID NO: 3321; SEQ ID NO: 3322; SEQ ID NO: 3323; SEQ ID NO: 3324; SEQ ID NO: 3325; SEQ ID NO: 3326; SEQ ID NO: 3327; SEQ ID NO: 3328; SEQ ID NO: 3329; SEQ ID NO: 3330; SEQ ID NO: 3331; SEQ ID NO: 3332; SEQ ID NO: 3333; SEQ ID NO: 3334; SEQ ID NO: 3335; SEQ ID NO: 3336; SEQ ID NO: 3337; SEQ ID NO: 3338; SEQ ID NO: 3339; SEQ ID NO: 3340; SEQ ID NO: 3341; SEQ ID NO: 3342; SEQ ID NO: 3343; SEQ ID NO: 3344; SEQ ID NO: 3345; SEQ ID NO: 3346; SEQ ID NO: 3347; SEQ ID NO: 3348; SEQ ID NO: 3349; SEQ ID NO: 3350; SEQ ID NO: 3351; SEQ ID NO: 3352; SEQ ID NO: 3353; SEQ ID NO: 3354; SEQ ID NO: 3355; SEQ ID NO: 3356; SEQ ID NO: 3357; SEQ ID NO: 3358; SEQ ID NO: 3359; SEQ ID NO: 3360; SEQ ID NO: 3361; SEQ ID NO: 3362; SEQ ID NO: 3363; SEQ ID NO: 3364; SEQ ID NO: 3365; SEQ ID NO: 3366; SEQ ID NO: 3367; SEQ ID NO: 3368; SEQ ID NO: 3369; SEQ ID NO: 3370; SEQ ID NO: 3371; SEQ ID NO: 3372; SEQ ID NO: 3373; SEQ ID NO: 3374; SEQ ID NO: 3375; SEQ ID NO: 3376; SEQ ID NO: 3377; SEQ ID NO: 3378; SEQ ID NO: 3379; SEQ ID NO: 3380; SEQ ID NO: 3381; SEQ ID NO: 3382; SEQ ID NO: 3383; SEQ ID NO: 3384; SEQ ID NO: 3385; SEQ ID NO: 3386; SEQ ID NO: 3387; SEQ ID NO: 3388; SEQ ID NO: 3389; SEQ ID NO: 3390; SEQ ID NO: 3391; SEQ ID NO: 3392; SEQ ID NO: 3393; SEQ ID NO: 3394; SEQ ID NO: 3395; SEQ ID NO: 3396; SEQ ID NO: 3397; SEQ ID NO: 3398; SEQ ID NO: 3399; SEQ ID NO: 3400; SEQ ID NO: 3401; SEQ ID NO: 3402; SEQ ID NO: 3403; SEQ ID NO: 3404; SEQ ID NO: 3405; SEQ ID NO: 3406; SEQ ID NO: 3407; SEQ ID NO: 3408; SEQ ID NO: 3409; SEQ ID NO: 3410; SEQ ID NO: 3411; SEQ ID NO: 3412; SEQ ID NO: 3413; SEQ ID NO: 3414; SEQ ID NO: 3415; SEQ ID NO: 3416; SEQ ID NO: 3417; SEQ ID NO: 3418; SEQ ID NO: 3419; SEQ ID NO: 3420; SEQ ID NO: 3421; SEQ ID NO: 3422; SEQ ID NO: 3423; SEQ ID NO: 3424; SEQ ID NO: 3425; SEQ ID NO: 3426; SEQ ID NO: 3427; SEQ ID NO: 3428; SEQ ID NO: 3429; SEQ ID NO: 3430; SEQ ID NO: 3431; SEQ ID NO: 3432; SEQ ID NO: 3433; SEQ ID NO: 3434; SEQ ID NO: 3435; SEQ ID NO: 3436; SEQ ID NO: 3437; SEQ ID NO: 3438; SEQ ID NO: 3439; SEQ ID NO: 3440; SEQ ID NO: 3441; SEQ ID NO: 3442; SEQ ID NO: 3443; SEQ ID NO: 3444; SEQ ID NO: 3445; SEQ ID NO: 3446; SEQ ID NO: 3447; SEQ ID NO: 3448; SEQ ID NO: 3449; SEQ ID NO: 3450; SEQ ID NO: 3451; SEQ ID NO: 3452; SEQ ID NO: 3453; SEQ ID NO: 3454; SEQ ID NO: 3455; SEQ ID NO: 3456; SEQ ID NO: 3457; SEQ ID NO: 3458; SEQ ID NO: 3459; SEQ ID NO: 3460; SEQ ID NO: 3461; SEQ ID NO: 3462; SEQ ID NO: 3463; SEQ ID NO: 3464; SEQ ID NO: 3465; SEQ ID NO: 3466; SEQ ID NO: 3467; SEQ ID NO: 3468; SEQ ID NO: 3469; SEQ ID NO: 3470; SEQ ID NO: 3471; SEQ ID NO: 3472; SEQ ID NO: 3473; SEQ ID NO: 3474; SEQ ID NO: 3475; SEQ ID NO: 3476; SEQ ID NO: 3477; SEQ ID NO: 3478; SEQ ID NO: 3479; SEQ ID NO: 3480; SEQ ID NO: 3481; SEQ ID NO: 3482; SEQ ID NO: 3483; SEQ ID NO: 3484; SEQ ID NO: 3485; SEQ ID NO: 3486; SEQ ID NO: 3487; SEQ ID NO: 3488; SEQ ID NO: 3489; SEQ ID NO: 3490; SEQ ID NO: 3491; SEQ ID NO: 3492; SEQ ID NO: 3493; SEQ ID NO: 3494; SEQ ID NO: 3495; SEQ ID NO: 3496; SEQ ID NO: 3497; SEQ ID NO: 3498; SEQ ID NO: 3499; SEQ ID NO: 3500; SEQ ID NO: 3501; SEQ ID NO: 3502; SEQ ID NO: 3503; SEQ ID NO: 3504; SEQ ID NO: 3505; SEQ ID NO: 3506; SEQ ID NO: 3507; SEQ ID NO: 3508; SEQ ID NO: 3509; SEQ ID NO: 3510; SEQ ID NO: 3511; SEQ ID NO: 3512; SEQ ID NO: 3513; SEQ ID NO: 3514; SEQ ID NO: 3515; SEQ ID NO: 3516; SEQ ID NO: 3517; SEQ ID NO: 3518; SEQ ID NO: 3519; SEQ ID NO: 3520; SEQ ID NO: 3521; SEQ ID NO: 3522; SEQ ID NO: 3523; SEQ ID NO: 3524; SEQ ID NO: 3525; SEQ ID NO: 3526; SEQ ID NO: 3527; SEQ ID NO: 3528; SEQ ID NO: 3529; SEQ ID NO: 3530; SEQ ID NO: 3531; SEQ ID NO: 3532; SEQ ID NO: 3533; SEQ ID NO: 3534; SEQ ID NO: 3535; SEQ ID NO: 3536; SEQ ID NO: 3537; SEQ ID NO: 3538; SEQ ID NO: 3539; SEQ ID NO: 3540; SEQ ID NO: 3541; SEQ ID NO: 3542; SEQ ID NO: 3543; SEQ ID NO: 3544; SEQ ID NO: 3545; SEQ ID NO: 3546; SEQ ID NO: 3547; SEQ ID NO: 3548; SEQ ID NO: 3549; SEQ ID NO: 3550; SEQ ID NO: 3551; SEQ ID NO: 3552; SEQ ID NO: 3553; SEQ ID NO: 3554; SEQ ID NO: 3555; SEQ ID NO: 3556; SEQ ID NO: 3557; SEQ ID NO: 3558; SEQ ID NO: 3559; SEQ ID NO: 3560; SEQ ID NO: 3561; SEQ ID NO: 3562; SEQ ID NO: 3563; SEQ ID NO: 3564; SEQ ID NO: 3565; SEQ ID NO: 3566; SEQ ID NO: 3567; SEQ ID NO: 3568; SEQ ID NO: 3569; SEQ ID NO: 3570; SEQ ID NO: 3571; SEQ ID NO: 3572; SEQ ID NO: 3573; SEQ ID NO: 3574; SEQ ID NO: 3575; SEQ ID NO: 3576; SEQ ID NO: 3577; SEQ ID NO: 3578; SEQ ID NO: 3579; SEQ ID NO: 3580; SEQ ID NO: 3581; SEQ ID NO: 3582; SEQ ID NO: 3583; SEQ ID NO: 3584; SEQ ID NO: 3585; SEQ ID NO: 3586; SEQ ID NO: 3587; SEQ ID NO: 3588; SEQ ID NO: 3589; SEQ ID NO: 3590; SEQ ID NO: 3591; SEQ ID NO: 3592; SEQ ID NO: 3593; SEQ ID NO: 3594; SEQ ID NO: 3595; SEQ ID NO: 3596; SEQ ID NO: 3597; SEQ ID NO: 3598; SEQ ID NO: 3599; SEQ ID NO: 3600; SEQ ID NO: 3601; SEQ ID NO: 3602; SEQ ID NO: 3603; SEQ ID NO: 3604; SEQ ID NO: 3605; SEQ ID NO: 3606; SEQ ID NO: 3607; SEQ ID NO: 3608; SEQ ID NO: 3609; SEQ ID NO: 3610; SEQ ID NO: 3611; SEQ ID NO: 3612; SEQ ID NO: 3613; SEQ ID NO 3614; SEQ ID NO: 3615; SEQ ID NO: 3616; SEQ ID NO: 3617; SEQ ID NO: 3618; SEQ ID NO: 3619; SEQ ID NO: 3620; SEQ ID NO: 3621; SEQ ID NO: 3622; SEQ ID NO: 3623; SEQ ID NO: 3624; SEQ ID NO: 3625; SEQ ID NO: 3626; SEQ ID NO: 3627; SEQ ID NO: 3628; SEQ ID NO: 3629; SEQ ID NO: 3630; SEQ ID NO: 3631; SEQ ID NO: 3632; SEQ ID NO: 3633; SEQ ID NO: 3634; SEQ ID NO: 3635; SEQ ID NO: 3636; SEQ ID NO: 3637; SEQ ID NO: 3638; SEQ ID NO: 3639; SEQ ID NO: 3640; SEQ ID NO: 3641; SEQ ID NO: 3642; SEQ ID NO: 3643; SEQ ID NO: 3644; SEQ ID NO: 3645; SEQ ID NO: 3646; SEQ ID NO: 3647; SEQ ID NO: 3648; SEQ ID NO: 3649; SEQ ID NO: 3650; SEQ ID NO: 3651; SEQ ID NO: 3652; SEQ ID NO: 3653; SEQ ID NO: 3654; SEQ ID NO: 3655; SEQ ID NO: 3656; SEQ ID NO: 3657; SEQ ID NO: 3658; SEQ ID NO: 3659; SEQ ID NO: 3660; SEQ ID NO: 3661; SEQ ID NO: 3662; SEQ ID NO: 3663; SEQ ID NO: 3664; SEQ ID NO: 3665; SEQ ID NO: 3666; SEQ ID NO: 3667; SEQ ID NO: 3668; SEQ ID NO: 3669; SEQ ID NO: 3670; SEQ ID NO: 3671; SEQ ID NO: 3672; SEQ ID NO: 3673; SEQ ID NO: 3674; SEQ ID NO: 3675; SEQ ID NO: 3676; SEQ ID NO: 3677; SEQ ID NO: 3678; SEQ ID NO: 3679; SEQ ID NO: 3680; SEQ ID NO: 3681; SEQ ID NO: 3682; SEQ ID NO: 3683; SEQ ID NO: 3684; SEQ ID NO: 3685; SEQ ID NO: 3686; SEQ ID NO: 3687; SEQ ID NO: 3688; SEQ ID NO: 3689; SEQ ID NO: 3690; SEQ ID NO: 3691; SEQ ID NO: 3692; SEQ ID NO: 3693; SEQ ID NO: 3694; SEQ ID NO: 3695; SEQ ID NO: 3696; SEQ ID NO: 3697; SEQ ID NO: 3698; SEQ ID NO: 3699; SEQ ID NO: 3700; SEQ ID NO: 3701; SEQ ID NO: 3702; SEQ ID NO: 3703; SEQ ID NO: 3704; SEQ ID NO: 3705; SEQ ID NO: 3706; SEQ ID NO: 3707; SEQ ID NO: 3708; SEQ ID NO: 3709; SEQ ID NO: 3710; SEQ ID NO: 3711; SEQ ID NO: 3712; SEQ ID NO: 3713; SEQ ID NO: 3714; SEQ ID NO: 3715; SEQ ID NO: 3716; SEQ ID NO: 3717; SEQ ID NO: 3718; SEQ ID NO: 3719; SEQ ID NO: 3720; SEQ ID NO: 3721; SEQ ID NO: 3722; SEQ ID NO: 3723; SEQ ID NO: 3724; SEQ ID NO: 3725; SEQ ID NO: 3726; SEQ ID NO: 3727; SEQ ID NO: 3728; SEQ ID NO: 3729; SEQ ID NO: 3730; SEQ ID NO: 3731; SEQ ID NO: 3732; SEQ ID NO: 3733; SEQ ID NO: 3734; SEQ ID NO: 3735; SEQ ID NO: 3736; SEQ ID NO: 3737; SEQ ID NO: 3738; SEQ ID NO: 3739; SEQ ID NO: 3740; SEQ ID NO: 3741; SEQ ID NO: 3742; SEQ ID NO: 3743; SEQ ID NO: 3744; SEQ ID NO: 3745; SEQ ID NO: 3746; SEQ ID NO: 3747; SEQ ID NO: 3748; SEQ ID NO: 3749; SEQ ID NO: 3750; SEQ ID NO: 3751; SEQ ID NO: 3752; SEQ ID NO: 3753; SEQ ID NO: 3754; SEQ ID NO: 3755; SEQ ID NO: 3756; SEQ ID NO: 3757; SEQ ID NO: 3758; SEQ ID NO: 3759; SEQ ID NO: 3760; SEQ ID NO: 3761; SEQ ID NO: 3762; SEQ ID NO: 3763; SEQ ID NO: 3764; SEQ ID NO: 3765; SEQ ID NO: 3766; SEQ ID NO: 3767; SEQ ID NO: 3768; SEQ ID NO: 3769; SEQ ID NO: 3770; SEQ ID NO: 3771; SEQ ID NO: 3772; SEQ ID NO: 3773; SEQ ID NO: 3774; SEQ ID NO: 3775; SEQ ID NO: 3776; SEQ ID NO: 3777; SEQ ID NO: 3778; SEQ ID NO: 3779; SEQ ID NO: 3780; SEQ ID NO: 3781; SEQ ID NO: 3782; SEQ ID NO: 3783; SEQ ID NO: 3784; SEQ ID NO: 3785; SEQ ID NO: 3786; SEQ ID NO: 3787; SEQ ID NO: 3788; SEQ ID NO: 3789; SEQ ID NO: 3790; SEQ ID NO: 3791; SEQ ID NO: 3792; SEQ ID NO: 3793; SEQ ID NO: 3794; SEQ ID NO: 3795; SEQ ID NO: 3796; SEQ ID NO: 3797; SEQ ID NO: 3798; SEQ ID NO: 3799; SEQ ID NO: 3800; SEQ ID NO: 3801; SEQ ID NO: 3802; SEQ ID NO: 3803; SEQ ID NO: 3804; SEQ ID NO: 3805; SEQ ID NO: 3806; SEQ ID NO: 3807; SEQ ID NO: 3808; SEQ ID NO: 3809; SEQ ID NO: 3810; SEQ ID NO: 3811; SEQ ID NO: 3812; SEQ ID NO: 3813; SEQ ID NO: 3814; SEQ ID NO: 3815; SEQ ID NO: 3816; SEQ ID NO: 3817; SEQ ID NO: 3818; SEQ ID NO: 3819; SEQ ID NO: 3820; SEQ ID NO: 3821; SEQ ID NO: 3822; SEQ ID NO: 3823; SEQ ID NO: 3824; SEQ ID NO: 3825; SEQ ID NO: 3826; SEQ ID NO: 3827; SEQ ID NO: 3828; SEQ ID NO: 3829; SEQ ID NO: 3830; SEQ ID NO: 3831; SEQ ID NO: 3832; SEQ ID NO: 3833; SEQ ID NO: 3834; SEQ ID NO: 3835; SEQ ID NO: 3836; SEQ ID NO: 3837; SEQ ID NO: 3838; SEQ ID NO: 3839; SEQ ID NO: 3840; SEQ ID NO: 3841; SEQ ID NO: 3842; SEQ ID NO: 3843; SEQ ID NO: 3844; SEQ ID NO: 3845; SEQ ID NO: 3846; SEQ ID NO: 3847; SEQ ID NO: 3848; SEQ ID NO: 3849; SEQ ID NO: 3850; SEQ ID NO: 3851; SEQ ID NO: 3852; SEQ ID NO: 3853; SEQ ID NO: 3854; SEQ ID NO: 3855; SEQ ID NO: 3856; SEQ ID NO: 3857; SEQ ID NO: 3858; SEQ ID NO: 3859; SEQ ID NO: 3860; SEQ ID NO: 3861; SEQ ID NO: 3862; SEQ ID NO: 3863; SEQ ID NO: 3864; SEQ ID NO: 3865; SEQ ID NO: 3866; SEQ ID NO: 3867; SEQ ID NO: 3868; SEQ ID NO: 3869; SEQ ID NO: 3870; SEQ ID NO: 3871; SEQ ID NO: 3872; SEQ ID NO: 3873; SEQ ID NO: 3874; SEQ ID NO: 3875; SEQ ID NO: 3876; SEQ ID NO: 3877; SEQ ID NO: 3878; SEQ ID NO: 3879; SEQ ID NO: 3880; SEQ ID NO: 3881; SEQ ID NO: 3882; SEQ ID NO: 3883; SEQ ID NO: 3884; SEQ ID NO: 3885; SEQ ID NO: 3886; SEQ ID NO: 3887; SEQ ID NO: 3888; SEQ ID NO: 3889; SEQ ID NO: 3890; SEQ ID NO: 3891; SEQ ID NO: 3892; SEQ ID NO: 3893; SEQ ID NO: 3894; SEQ ID NO: 3895; SEQ ID NO: 3896; SEQ ID NO: 3897; SEQ ID NO: 3898; SEQ ID NO: 3899; SEQ ID NO: 3900; SEQ ID NO: 3901; SEQ ID NO: 3902; SEQ ID NO: 3903; SEQ ID NO: 3904; SEQ ID NO: 3905; SEQ ID NO: 3906; SEQ ID NO: 3907; SEQ ID NO: 3908; SEQ ID NO: 3909; SEQ ID NO: 3910; SEQ ID NO: 3911; SEQ ID NO: 3912; SEQ ID NO: 3913; SEQ ID NO: 3914; SEQ ID NO: 3915; SEQ ID NO: 3916; SEQ ID NO: 3917; SEQ ID NO: 3918; SEQ ID NO: 3919; SEQ ID NO: 3920; SEQ ID NO: 3921; SEQ ID NO: 3922; SEQ ID NO: 3923; SEQ ID NO: 3924; SEQ ID NO: 3925; SEQ ID NO: 3926; SEQ ID NO: 3927; SEQ ID NO: 3928; SEQ ID NO: 3929; SEQ ID NO: 3930; SEQ ID NO: 3931; SEQ ID NO: 3932; SEQ ID NO: 3933; SEQ ID NO: 3934; SEQ ID NO: 3935; SEQ ID NO: 3936; SEQ ID NO: 3937; SEQ ID NO: 3938; SEQ ID NO: 3939; SEQ ID NO: 3940; SEQ ID NO: 3941; SEQ ID NO: 3942; SEQ ID NO: 3943; SEQ ID NO: 3944; SEQ ID NO: 3945; SEQ ID NO: 3946; SEQ ID NO: 3947; SEQ ID NO: 3948; SEQ ID NO: 3949; SEQ ID NO: 3950; SEQ ID NO: 3951; SEQ ID NO: 3952; SEQ ID NO: 3953; SEQ ID NO: 3954; SEQ ID NO: 3955; SEQ ID NO: 3956; SEQ ID NO: 3957; SEQ ID NO: 3958; SEQ ID NO: 3959; SEQ ID NO: 3960; SEQ ID NO: 3961; SEQ ID NO: 3962; SEQ ID NO: 3963; SEQ ID NO: 3964; SEQ ID NO: 3965; SEQ ID NO: 3966; SEQ ID NO: 3967; SEQ ID NO: 3968; SEQ ID NO: 3969; SEQ ID NO: 3970; SEQ ID NO: 3971; SEQ ID NO: 3972; SEQ ID NO: 3973; SEQ ID NO: 3974; SEQ ID NO: 3975; SEQ ID NO: 3976; SEQ ID NO: 3977; SEQ ID NO: 3978; SEQ ID NO: 3979; SEQ ID NO: 3980; SEQ ID NO: 3981; SEQ ID NO: 3982; SEQ ID NO: 3983; SEQ ID NO: 3984; SEQ ID NO: 3985; SEQ ID NO: 3986; SEQ ID NO: 3987; SEQ ID NO: 3988; SEQ ID NO: 3989; SEQ ID NO: 3990; SEQ ID NO: 3991; SEQ ID NO: 3992; SEQ ID NO: 3993; SEQ ID NO: 3994; SEQ ID NO: 3995; SEQ ID NO: 3996; SEQ ID NO: 3997; SEQ ID NO: 3998; SEQ ID NO: 3999; SEQ ID NO: 4000; SEQ ID NO: 4001; SEQ ID NO: 4002; SEQ ID NO: 4003; SEQ ID NO: 4004; SEQ ID NO: 4005; SEQ ID NO: 4006; SEQ ID NO: 4007; SEQ ID NO: 4008; SEQ ID NO: 4009; SEQ ID NO: 4010; SEQ ID NO: 4011; SEQ ID NO: 4012; SEQ ID NO: 4013; SEQ ID NO: 4014; SEQ ID NO: 4015; SEQ ID NO: 9593; SEQ ID NO: 9594; SEQ ID NO: 9595; SEQ ID NO: 9596; SEQ ID NO: 9597; SEQ ID NO: 9598; SEQ ID NO: 9599; SEQ ID NO: 9600; SEQ ID NO: 9601; SEQ ID NO: 9602; SEQ ID NO: 9603; SEQ ID NO: 9604; SEQ ID NO: 9605; SEQ ID NO: 9606; SEQ ID NO: 9607; SEQ ID NO: 9608; SEQ ID NO: 9609; SEQ ID NO: 9610; SEQ ID NO: 9611; SEQ ID NO: 9612; SEQ ID NO: 9613; SEQ ID NO: 9614; SEQ ID NO: 9615; SEQ ID NO: 9616; SEQ ID NO: 9617; SEQ ID NO: 9618; SEQ ID NO: 9619; SEQ ID NO: 9620 and SEQ ID NO: 9621.  
     [0148] In one embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in energy metabolism or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1576; SEQ ID NO: 1576; SEQ ID NO: 1577; SEQ ID NO: 1578; SEQ ID NO: 1579; SEQ ID NO: 1580; SEQ ID NO: 1581; SEQ ID NO: 1582; SEQ ID NO: 1583; SEQ ID NO: 1584; SEQ ID NO: 1585; SEQ ID NO: 1586; SEQ ID NO: 1587; SEQ ID NO: 1588; SEQ ID NO: 1589; SEQ ID NO: 1590; SEQ ID NO: 1591; SEQ ID NO: 1592; SEQ ID NO: 1593; SEQ ID NO: 1594; SEQ ID NO: 1595; SEQ ID NO: 1596; SEQ ID NO: 1597; SEQ ID NO: 1598; SEQ ID NO: 1599; SEQ ID NO: 1600; SEQ ID NO: 1601; SEQ ID NO: 1602; SEQ ID NO: 1603; SEQ ID NO: 1604; SEQ ID NO: 1605; SEQ ID NO: 1606; SEQ ID NO: 1607; SEQ ID NO: 1608; SEQ ID NO: 1609; SEQ ID NO: 1610; SEQ ID NO: 1611; SEQ ID NO: 1612; SEQ ID NO: 1613; SEQ ID NO: 1614; SEQ ID NO: 1615; SEQ ID NO: 1616; SEQ ID NO: 1617; SEQ ID NO: 1618; SEQ ID NO: 1619; SEQ ID NO: 1620; SEQ ID NO: 1621; SEQ ID NO: 1622; SEQ ID NO: 1623; SEQ ID NO: 1624; SEQ ID NO: 1625; SEQ ID NO: 1626; SEQ ID NO: 1627; SEQ ID NO: 1628; SEQ ID NO: 1629; SEQ ID NO: 1630; SEQ ID NO: 1631; SEQ ID NO: 1632; SEQ ID NO: 1633; SEQ ID NO: 1634; SEQ ID NO: 1635; SEQ ID NO: 1636; SEQ ID NO: 1637; SEQ ID NO: 1638; SEQ ID NO: 1639; SEQ ID NO: 1640; SEQ ID NO: 1641; SEQ ID NO: 1642; SEQ ID NO: 1643; SEQ ID NO: 1644; SEQ ID NO: 1645; SEQ ID NO: 1646; SEQ ID NO: 1647; SEQ ID NO: 1648; SEQ ID NO: 1649; SEQ ID NO: 1650; SEQ ID NO: 1651; SEQ ID NO: 1652; SEQ ID NO: 1653; SEQ ID NO: 1654; SEQ ID NO: 1655; SEQ ID NO: 1656; SEQ ID NO: 1657; SEQ ID NO: 1658; SEQ ID NO: 1659; SEQ ID NO: 1660; SEQ ID NO: 1661; SEQ ID NO: 1662; SEQ ID NO: 1663; SEQ ID NO: 1664; SEQ ID NO: 1665; SEQ ID NO: 1666; SEQ ID NO: 1667; SEQ ID NO: 1668; SEQ ID NO: 1669; SEQ ID NO: 1670; SEQ ID NO: 1671; SEQ ID NO: 1672; SEQ ID NO: 1673; SEQ ID NO: 1674; SEQ ID NO: 1675; SEQ ID NO: 1676; SEQ ID NO: 1677; SEQ ID NO: 1678; SEQ ID NO: 1679; SEQ ID NO: 1680; SEQ ID NO: 1681; SEQ ID NO: 1682; SEQ ID NO: 1683; SEQ ID NO: 1684; SEQ ID NO: 1685; SEQ ID NO: 1686; SEQ ID NO: 1687; SEQ ID NO: 1688; SEQ ID NO: 1689; SEQ ID NO: 1690; SEQ ID NO: 1691; SEQ ID NO: 1692; SEQ ID NO: 1693; SEQ ID NO: 1694; SEQ ID NO: 1695; SEQ ID NO: 1696; SEQ ID NO: 1697; SEQ ID NO: 1698; SEQ ID NO: 1699; SEQ ID NO: 1700; SEQ ID NO: 1701; SEQ ID NO: 1702; SEQ ID NO: 1703; SEQ ID NO: 1704; SEQ ID NO: 1705; SEQ ID NO: 1706; SEQ ID NO: 1707; SEQ ID NO: 1708; SEQ ID NO: 1709; SEQ ID NO: 1710; SEQ ID NO: 1711; SEQ ID NO: 1712; SEQ ID NO: 1713; SEQ ID NO: 1714; SEQ ID NO: 1715; SEQ ID NO: 1716; SEQ ID NO: 1717; SEQ ID NO: 1718; SEQ ID NO: 1719; SEQ ID NO: 1720; SEQ ID NO: 1721; SEQ ID NO: 1722; SEQ ID NO: 1723; SEQ ID NO: 1724; SEQ ID NO: 1725; SEQ ID NO: 9593 and SEQ ID NO: 9594.  
     [0149] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in amino acid metabolism or a fragment thereof encoded by a nucleic acid having a sequence selected from the group consisting of SEQ ID NO: 1726; SEQ ID NO: 1727; SEQ ID NO: 1728; SEQ ID NO: 1729; SEQ ID NO: 1730; SEQ ID NO: 1731; SEQ ID NO: 1732; SEQ ID NO: 1733; SEQ ID NO: 1734; SEQ ID NO: 1735; SEQ ID NO: 1736; SEQ ID NO: 1737; SEQ ID NO: 1738; SEQ ID NO: 1739; SEQ ID NO: 1740; SEQ ID NO: 1741; SEQ ID NO: 1742; SEQ ID NO: 1743; SEQ ID NO: 1744; SEQ ID NO: 1745; SEQ ID NO: 1746; SEQ ID NO: 1747; SEQ ID NO: 1748; SEQ ID NO: 1749; SEQ ID NO: 1750; SEQ ID NO: 1751; SEQ ID NO: 1752; SEQ ID NO: 1753; SEQ ID NO: 1754; SEQ ID NO: 1755; SEQ ID NO: 1756; SEQ ID NO: 1757; SEQ ID NO: 1758; SEQ ID NO: 1759; SEQ ID NO: 1760; SEQ ID NO: 1761; SEQ ID NO: 1762; SEQ ID NO: 1763; SEQ ID NO: 1764; SEQ ID NO: 1765; SEQ ID NO: 1766; SEQ ID NO: 1767; SEQ ID NO: 1768; SEQ ID NO: 1769; SEQ ID NO: 1770; SEQ ID NO: 1771; SEQ ID NO: 1772; SEQ ID NO: 1773; SEQ ID NO: 1774; SEQ ID NO: 1775; SEQ ID NO: 1776; SEQ ID NO: 1777; SEQ ID NO: 1778; SEQ ID NO: 1779; SEQ ID NO: 1780; SEQ ID NO: 1781; SEQ ID NO: 1782; SEQ ID NO: 1783; SEQ ID NO: 1784; SEQ ID NO: 1785; SEQ ID NO: 1786; SEQ ID NO: 1787; SEQ ID NO: 1788; SEQ ID NO: 1789; SEQ ID NO: 1790; SEQ ID NO: 1791; SEQ ID NO: 1792; SEQ ID NO: 1793; SEQ ID NO: 1794; SEQ ID NO: 1795; SEQ ID NO: 1796; SEQ ID NO: 1797; SEQ ID NO: 1798; SEQ ID NO: 1799; SEQ ID NO: 1800; SEQ ID NO: 1801; SEQ ID NO: 1802; SEQ ID NO: 1803; SEQ ID NO: 1804; SEQ ID NO: 1805; SEQ ID NO: 1806; SEQ ID NO: 1807; SEQ ID NO: 1808; SEQ ID NO: 1809; SEQ ID NO: 1810; SEQ ID NO: 1811; SEQ ID NO: 1812; SEQ ID NO: 1813; SEQ ID NO: 1814; SEQ ID NO: 1815; SEQ ID NO: 1816; SEQ ID NO: 1817; SEQ ID NO: 1818; SEQ ID NO: 1819; SEQ ID NO: 1820; SEQ ID NO: 1821; SEQ ID NO: 1822; SEQ ID NO: 1823; SEQ ID NO: 1824; SEQ ID NO: 1825; SEQ ID NO: 1826; SEQ ID NO: 1827; SEQ ID NO: 1828; SEQ ID NO: 1829; SEQ ID NO: 1830; SEQ ID NO: 1831; SEQ ID NO: 1832; SEQ ID NO: 1833; SEQ ID NO: 1834; SEQ ID NO: 1835; SEQ ID NO: 1836; SEQ ID NO: 1837; SEQ ID NO: 1838; SEQ ID NO: 1839; SEQ ID NO: 1840; SEQ ID NO: 1841; SEQ ID NO: 1842; SEQ ID NO: 1843; SEQ ID NO: 1844; SEQ ID NO: 1845; SEQ ID NO: 1846; SEQ ID NO: 1847; SEQ ID NO: 1848; SEQ ID NO: 1849; SEQ ID NO: 1850; SEQ ID NO: 1851; SEQ ID NO: 1852; SEQ ID NO: 1853; SEQ ID NO: 1854; SEQ ID NO: 1855; SEQ ID NO: 1856; SEQ ID NO: 1857; SEQ ID NO: 1858; SEQ ID NO: 1859; SEQ ID NO: 1860; SEQ ID NO: 1861; SEQ ID NO: 1862; SEQ ID NO: 1863; SEQ ID NO: 1864; SEQ ID NO: 1865; SEQ ID NO: 1866; SEQ ID NO: 1867; SEQ ID NO: 1868; SEQ ID NO: 9595 and SEQ ID NO: 9596.  
     [0150] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in cofactor metabolism or a fragment thereof encoded by a nucleic acid having a sequence selected from the group consisting of SEQ ID NO: 1869; SEQ ID NO: 1870; SEQ ID NO: 1871; SEQ ID NO: 1872; SEQ ID NO: 1873; SEQ ID NO: 1874; SEQ ID NO: 1875; SEQ ID NO: 1876; SEQ ID NO: 1877; SEQ ID NO: 1878; SEQ ID NO: 1879; SEQ ID NO: 1880; SEQ ID NO: 1881; SEQ ID NO: 1882; SEQ ID NO: 1883; SEQ ID NO: 1884; SEQ ID NO: 1885; SEQ ID NO: 1886; SEQ ID NO: 1887; SEQ ID NO: 1888; SEQ ID NO: 1889; SEQ ID NO: 1890; SEQ ID NO: 1891; SEQ ID NO: 1892; SEQ ID NO: 1893; SEQ ID NO: 1894; SEQ ID NO: 1895; SEQ ID NO: 1896; SEQ ID NO: 1897; SEQ ID NO: 1898; SEQ ID NO: 1899; SEQ ID NO: 1900; SEQ ID NO: 1901; SEQ ID NO: 1902; SEQ ID NO: 1903; SEQ ID NO: 1904; SEQ ID NO: 1905; SEQ ID NO: 1906; SEQ ID NO: 1907; SEQ ID NO: 1908; SEQ ID NO: 1909; SEQ ID NO: 1910; SEQ ID NO: 1911; SEQ ID NO: 1912; SEQ ID NO: 1913; SEQ ID NO: 1914; SEQ ID NO: 1915; SEQ ID NO: 1916; SEQ ID NO: 1917; SEQ ID NO: 1918; SEQ ID NO: 1919; SEQ ID NO: 1920; SEQ ID NO: 1921; SEQ ID NO: 1922; SEQ ID NO: 1923; SEQ ID NO: 1924; SEQ ID NO: 1925; SEQ ID NO: 1926; SEQ ID NO: 1927; SEQ ID NO: 1928; SEQ ID NO: 1929; SEQ ID NO: 1930; SEQ ID NO: 1931; SEQ ID NO: 1932; SEQ ID NO: 1933; SEQ ID NO: 1934; SEQ ID NO: 1935; SEQ ID NO: 1936; SEQ ID NO: 1937; SEQ ID NO: 1938; SEQ ID NO: 1939; SEQ ID NO: 1940; SEQ ID NO: 1941; SEQ ID NO: 1942; SEQ ID NO: 1943; SEQ ID NO: 1944; SEQ ID NO: 1945; SEQ ID NO: 1946; SEQ ID NO: 1947; SEQ ID NO: 1948; SEQ ID NO: 1949; SEQ ID NO: 1950; SEQ ID NO: 1951; SEQ ID NO: 1952; SEQ ID NO: 1953; SEQ ID NO: 1954; SEQ ID NO: 1955; SEQ ID NO: 1956; SEQ ID NO: 1957; SEQ ID NO: 1958; SEQ ID NO: 1959; SEQ ID NO: 1960; SEQ ID NO: 1961; SEQ ID NO: 1962; SEQ ID NO: 1963; SEQ ID NO: 1964; SEQ ID NO: 1965; SEQ ID NO: 1966; SEQ ID NO: 1967; SEQ ID NO: 1968; SEQ ID NO: 1969; SEQ ID NO: 1970; SEQ ID NO: 1971; SEQ ID NO: 1972; SEQ ID NO: 1973; SEQ ID NO: 1974; SEQ ID NO: 1975; SEQ ID NO: 1976; SEQ ID NO: 1977; SEQ ID NO: 1978; SEQ ID NO: 1979; SEQ ID NO: 1980; SEQ ID NO: 1981; SEQ ID NO: 1982; SEQ ID NO: 1983; SEQ ID NO: 1984; SEQ ID NO: 1985; SEQ ID NO: 1986; SEQ ID NO: 1987; SEQ ID NO: 1988; SEQ ID NO: 1989; SEQ ID NO: 1990; SEQ ID NO: 1991; SEQ ID NO: 1992; SEQ ID NO: 1993; SEQ ID NO: 1994; SEQ ID NO: 1995; SEQ ID NO: 1996; SEQ ID NO: 1997; SEQ ID NO: 1998; SEQ ID NO: 1999; SEQ ID NO: 2000; SEQ ID NO: 2001; SEQ ID NO: 2002; SEQ ID NO: 2003; SEQ ID NO: 2004; SEQ ID NO: 2005; SEQ ID NO: 9597.  
     [0151] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in carbohydrate metabolism or a fragment thereof encoded by a nucleic acid having a sequence selected from the group consisting of SEQ ID NO: 2006; SEQ ID NO: 2007; SEQ ID NO: 2008; SEQ ID NO: 2009; SEQ ID NO: 2010; SEQ ID NO: 2011; SEQ ID NO: 2012; SEQ ID NO: 2013; SEQ ID NO: 2014; SEQ ID NO: 2015; SEQ ID NO: 2016; SEQ ID NO: 2017; SEQ ID NO: 2018; SEQ ID NO: 2019; SEQ ID NO: 2020; SEQ ID NO: 2021; SEQ ID NO: 2022; SEQ ID NO: 2023; SEQ ID NO: 2024; SEQ ID NO: 2025; SEQ ID NO: 2026; SEQ ID NO: 2027; SEQ ID NO: 2028; SEQ ID NO: 2029; SEQ ID NO: 2030; SEQ ID NO: 2031; SEQ ID NO: 2032; SEQ ID NO: 2033; SEQ ID NO: 2034; SEQ ID NO: 2035; SEQ ID NO: 2036; SEQ ID NO: 2037; SEQ ID NO: 2038; SEQ ID NO: 2039; SEQ ID NO: 2040; SEQ ID NO: 2041; SEQ ID NO: 2042; SEQ ID NO: 2043; SEQ ID NO: 2044; SEQ ID NO: 2045; SEQ ID NO: 2046; SEQ ID NO: 2047; SEQ ID NO: 2048; SEQ ID NO: 2049; SEQ ID NO: 2050; SEQ ID NO: 2051; SEQ ID NO: 2052; SEQ ID NO: 2053; SEQ ID NO: 2054; SEQ ID NO: 2055; SEQ ID NO: 2056; SEQ ID NO: 2057; SEQ ID NO: 2058; SEQ ID NO: 2059; SEQ ID NO: 2060; SEQ ID NO: 2061; SEQ ID NO: 2062; SEQ ID NO: 2063; SEQ ID NO: 2064; SEQ ID NO: 2065; SEQ ID NO: 2066; SEQ ID NO: 2067; SEQ ID NO: 2068; SEQ ID NO: 2069; SEQ ID NO: 2070; SEQ ID NO: 2071; SEQ ID NO: 2072; SEQ ID NO: 2073; SEQ ID NO: 2074; SEQ ID NO: 2075; SEQ ID NO: 2076; SEQ ID NO: 9598; SEQ ID NO: 9599.  
     [0152] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in nucleic acid metabolism or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2077; SEQ ID NO: 2078; SEQ ID NO: 2079; SEQ ID NO: 2080; SEQ ID NO: 2081; SEQ ID NO: 2082; SEQ ID NO: 2083; SEQ ID NO: 2084; SEQ ID NO: 2085; SEQ ID NO: 2086; SEQ ID NO: 2087; SEQ ID NO: 2088; SEQ ID NO: 2089; SEQ ID NO: 2090; SEQ ID NO: 2091; SEQ ID NO: 2092; SEQ ID NO: 2093; SEQ ID NO: 2094; SEQ ID NO: 2095; SEQ ID NO: 2096; SEQ ID NO: 2097; SEQ ID NO: 2098; SEQ ID NO: 2099; SEQ ID NO: 2100; SEQ ID NO: 2101; SEQ ID NO: 2102; SEQ ID NO: 2103; SEQ ID NO: 2104; SEQ ID NO: 2105; SEQ ID NO: 2106; SEQ ID NO: 2107; SEQ ID NO: 2108; SEQ ID NO: 2109; SEQ ID NO: 2110; SEQ ID NO: 2111; SEQ ID NO: 2112; SEQ ID NO: 2113; SEQ ID NO: 2114; SEQ ID NO: 2115; SEQ ID NO: 2116; SEQ ID NO: 2117; SEQ ID NO: 2118; SEQ ID NO: 2119; SEQ ID NO: 2120; SEQ ID NO: 2121; SEQ ID NO: 2122; SEQ ID NO: 2123; SEQ ID NO: 2124; SEQ ID NO: 2125; SEQ ID NO: 2126; SEQ ID NO: 2127; SEQ ID NO: 2128; SEQ ID NO: 2129; SEQ ID NO: 2130; SEQ ID NO: 2131; SEQ ID NO: 2132; SEQ ID NO: 2133; SEQ ID NO: 2134; SEQ ID NO: 2135; SEQ ID NO: 2136; SEQ ID NO: 2137; SEQ ID NO: 2138; SEQ ID NO: 2139; SEQ ID NO: 2140; SEQ ID NO: 2141; SEQ ID NO: 2142; SEQ ID NO: 2143; SEQ ID NO: 2144; SEQ ID NO: 2145; SEQ ID NO: 2146; SEQ ID NO: 2147; SEQ ID NO: 2148; SEQ ID NO: 2149; SEQ ID NO: 2150; SEQ ID NO: 2151; SEQ ID NO: 2152; SEQ ID NO: 2153; SEQ ID NO: 2154; SEQ ID NO: 2155; SEQ ID NO: 2156; SEQ ID NO: 2157; SEQ ID NO: 2158; SEQ ID NO: 2159; SEQ ID NO: 2160; SEQ ID NO: 2161; SEQ ID NO: 2162; SEQ ID NO: 2163; SEQ ID NO: 2164; SEQ ID NO: 2165; SEQ ID NO: 2166; SEQ ID NO: 2167; SEQ ID NO: 2168; SEQ ID NO: 2169; SEQ ID NO: 2170; SEQ ID NO: 2171; SEQ ID NO: 2172; SEQ ID NO: 2173; SEQ ID NO: 2174; SEQ ID NO: 2175; SEQ ID NO: 2176; SEQ ID NO: 2177; SEQ ID NO: 2178; SEQ ID NO: 2179; SEQ ID NO: 2180; SEQ ID NO: 2181; SEQ ID NO: 2182; SEQ ID NO: 9600.  
     [0153] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in lipid metabolism or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2183; SEQ ID NO: 2184; SEQ ID NO: 2185; SEQ ID NO: 2186; SEQ ID NO: 2187; SEQ ID NO: 2188; SEQ ID NO: 2189; SEQ ID NO: 2190; SEQ ID NO: 2191; SEQ ID NO: 2192; SEQ ID NO: 2193; SEQ ID NO: 2194; SEQ ID NO: 2195; SEQ ID NO: 2196; SEQ ID NO: 2197; SEQ ID NO: 2198; SEQ ID NO: 2199; SEQ ID NO: 2200; SEQ ID NO: 2201; SEQ ID NO: 2202; SEQ ID NO: 2203; SEQ ID NO: 2204; SEQ ID NO: 2205; SEQ ID NO: 2206; SEQ ID NO: 2207; SEQ ID NO: 2208; SEQ ID NO: 2209; SEQ ID NO: 2210; SEQ ID NO: 2211; SEQ ID NO: 2212; SEQ ID NO: 2213; SEQ ID NO: 2214; SEQ ID NO: 2215; SEQ ID NO: 2216; SEQ ID NO: 2217; SEQ ID NO: 2218; SEQ ID NO: 9601 and SEQ ID NO: 9602.  
     [0154] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in mRNA translation and ribosome biogenesis or a fragment thereof encoded by a nucleic acid having a sequence selected from the group consisting of SEQ ID NO: 2219; SEQ ID NO: 2220; SEQ ID NO: 2221; SEQ ID NO: 2222; SEQ ID NO: 2223; SEQ ID NO: 2224; SEQ ID NO: 2225; SEQ ID NO: 2226; SEQ ID NO: 2227; SEQ ID NO: 2228; SEQ ID NO: 2229; SEQ ID NO: 2230; SEQ ID NO: 2231; SEQ ID NO: 2232; SEQ ID NO: 2233; SEQ ID NO: 2234; SEQ ID NO: 2235; SEQ ID NO: 2236; SEQ ID NO: 2237; SEQ ID NO: 2238; SEQ ID NO: 2239; SEQ ID NO: 2240; SEQ ID NO: 2241; SEQ ID NO: 2242; SEQ ID NO: 2243; SEQ ID NO: 2244; SEQ ID NO: 2245; SEQ ID NO: 2246; SEQ ID NO: 2247; SEQ ID NO: 2248; SEQ ID NO: 2249; SEQ ID NO: 2250; SEQ ID NO: 2251; SEQ ID NO: 2252; SEQ ID NO: 2253; SEQ ID NO: 2254; SEQ ID NO: 2255; SEQ ID NO: 2256; SEQ ID NO: 2257; SEQ ID NO: 2258; SEQ ID NO: 2259; SEQ ID NO: 2260; SEQ ID NO: 2261; SEQ ID NO: 2262; SEQ ID NO: 2263; SEQ ID NO: 2264; SEQ ID NO: 2265; SEQ ID NO: 2266; SEQ ID NO: 2267; SEQ ID NO: 2268; SEQ ID NO: 2269; SEQ ID NO: 2270; SEQ ID NO: 2271; SEQ ID NO: 2272; SEQ ID NO: 2273; SEQ ID NO: 2274; SEQ ID NO: 2275; SEQ ID NO: 2276; SEQ ID NO: 2277; SEQ ID NO: 2278; SEQ ID NO: 2279; SEQ ID NO: 2280; SEQ ID NO: 2281; SEQ ID NO: 2282; SEQ ID NO: 2283; SEQ ID NO: 2284; SEQ ID NO: 2285; SEQ ID NO: 2286; SEQ ID NO: 2287; SEQ ID NO: 2288; SEQ ID NO: 2289; SEQ ID NO: 2290; SEQ ID NO: 2291; SEQ ID NO: 2292; SEQ ID NO: 2293; SEQ ID NO: 2294; SEQ ID NO: 2295; SEQ ID NO: 2296; SEQ ID NO: 2297; SEQ ID NO: 2298; SEQ ID NO: 2299; SEQ ID NO: 2300; SEQ ID NO: 2301; SEQ ID NO: 2302; SEQ ID NO: 2303; SEQ ID NO: 2304; SEQ ID NO: 2305; SEQ ID NO: 2306; SEQ ID NO: 2307; SEQ ID NO: 2308; SEQ ID NO: 2309; SEQ ID NO: 2310; SEQ ID NO: 2311; SEQ ID NO: 2312; SEQ ID NO: 2313; SEQ ID NO: 2314; SEQ ID NO: 2315; SEQ ID NO: 2316; SEQ ID NO: 2317; SEQ ID NO: 2318; SEQ ID NO: 2319; SEQ ID NO: 2320; SEQ ID NO: 2321; SEQ ID NO: 2322; SEQ ID NO: 2323; SEQ ID NO: 2324; SEQ ID NO: 2325; SEQ ID NO: 2326; SEQ ID NO: 2327; SEQ ID NO: 2328; SEQ ID NO: 2329; SEQ ID NO: 2330; SEQ ID NO: 2331; SEQ ID NO: 2332; SEQ ID NO: 2333; SEQ ID NO: 2334; SEQ ID NO: 2335; SEQ ID NO: 2336; SEQ ID NO: 2337; SEQ ID NO: 2338; SEQ ID NO: 2339; SEQ ID NO: 2340; SEQ ID NO: 2341; SEQ ID NO: 2342; SEQ ID NO: 2343; SEQ ID NO: 2344; SEQ ID NO: 2345; SEQ ID NO: 2346; SEQ ID NO: 2347; SEQ ID NO: 2348; SEQ ID NO: 2349; SEQ ID NO: 2350; SEQ ID NO: 2351; SEQ ID NO: 2352; SEQ ID NO: 2353; SEQ ID NO: 2354; SEQ ID NO: 2355; SEQ ID NO: 2356; SEQ ID NO: 2357; SEQ ID NO: 2358; SEQ ID NO: 2359; SEQ ID NO: 2360; SEQ ID NO: 2361; SEQ ID NO: 2362; SEQ ID NO: 2363; SEQ ID NO: 2364; SEQ ID NO: 2365; SEQ ID NO: 2366; SEQ ID NO: 2367; SEQ ID NO: 2368; SEQ ID NO: 2369; SEQ ID NO: 2370; SEQ ID NO: 2371; SEQ ID NO: 2372; SEQ ID NO: 2373; SEQ ID NO: 2374; SEQ ID NO: 2375; SEQ ID NO: 2376; SEQ ID NO: 2377; SEQ ID NO: 2378; SEQ ID NO: 2379; SEQ ID NO: 2380; SEQ ID NO: 2381; SEQ ID NO: 2382; SEQ ID NO: 2383; SEQ ID NO: 2384; SEQ ID NO: 2385; SEQ ID NO: 2386; SEQ ID NO: 2387; SEQ ID NO: 2388; SEQ ID NO: 2389; SEQ ID NO: 2390; SEQ ID NO: 2391; SEQ ID NO: 2392; SEQ ID NO: 2393; SEQ ID NO: 2394; SEQ ID NO: 2395; SEQ ID NO: 2396; SEQ ID NO: 2397; SEQ ID NO: 2398; SEQ ID NO: 2399; SEQ ID NO: 2400; SEQ ID NO: 2401; SEQ ID NO: 2402; SEQ ID NO: 2403; SEQ ID NO: 2404; SEQ ID NO: 2405; SEQ ID NO: 2406; SEQ ID NO: 2407; SEQ ID NO: 2408; SEQ ID NO: 2409; SEQ ID NO: 2410; SEQ ID NO: 2411; SEQ ID NO: 2412; SEQ ID NO: 2413; SEQ ID NO: 2414; SEQ ID NO: 2415; SEQ ID NO: 2416; SEQ ID NO: 2417; SEQ ID NO: 2418; SEQ ID NO: 2419; SEQ ID NO: 2420; SEQ ID NO: 2421; SEQ ID NO: 2422; SEQ ID NO: 2423; SEQ ID NO: 2424; SEQ ID NO: 2425; SEQ ID NO: 2426; SEQ ID NO: 2427; SEQ ID NO: 2428; SEQ ID NO: 2429; SEQ ID NO: 2430; SEQ ID NO: 2431; SEQ ID NO: 2432; SEQ ID NO: 2433; SEQ ID NO: 2434; SEQ ID NO: 2435; SEQ ID NO: 2436; SEQ ID NO: 2437; SEQ ID NO: 2438; SEQ ID NO: 9603 and SEQ ID NO: 9604.  
     [0155] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in genome replication, transcription, recombination and repair or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 2439; SEQ ID NO: 2440; SEQ ID NO: 2441; SEQ ID NO: 2442; SEQ ID NO: 2443; SEQ ID NO: 2444; SEQ ID NO: 2445; SEQ ID NO: 2446; SEQ ID NO: 2447; SEQ ID NO: 2448; SEQ ID NO: 2449; SEQ ID NO: 2450; SEQ ID NO: 2451; SEQ ID NO: 2452; SEQ ID NO: 2453; SEQ ID NO: 2454; SEQ ID NO: 2455; SEQ ID NO: 2456; SEQ ID NO: 2457; SEQ ID NO: 2458; SEQ ID NO: 2459; SEQ ID NO: 2460; SEQ ID NO: 2461; SEQ ID NO: 2462; SEQ ID NO: 2463; SEQ ID NO: 2464; SEQ ID NO: 2465; SEQ ID NO: 2466; SEQ ID NO: 2467; SEQ ID NO: 2468; SEQ ID NO: 2469; SEQ ID NO: 2470; SEQ ID NO: 2471; SEQ ID NO: 2472; SEQ ID NO: 2473; SEQ ID NO: 2474; SEQ ID NO: 2475; SEQ ID NO: 2476; SEQ ID NO: 2477; SEQ ID NO: 2478; SEQ ID NO: 2479; SEQ ID NO: 2480; SEQ ID NO: 2481; SEQ ID NO: 2482; SEQ ID NO: 2483; SEQ ID NO: 2484; SEQ ID NO: 2485; SEQ ID NO: 2486; SEQ ID NO: 2487; SEQ ID NO: 2488; SEQ ID NO: 2489; SEQ ID NO: 2490; SEQ ID NO: 2491; SEQ ID NO: 2492; SEQ ID NO: 2493; SEQ ID NO: 2494; SEQ ID NO: 2495; SEQ ID NO: 2496; SEQ ID NO: 2497; SEQ ID NO: 2498; SEQ ID NO: 2499; SEQ ID NO: 2500; SEQ ID NO: 2501; SEQ ID NO: 2502; SEQ ID NO: 2503; SEQ ID NO: 2504; SEQ ID NO: 2505; SEQ ID NO: 2506; SEQ ID NO: 2507; SEQ ID NO: 2508; SEQ ID NO: 2509; SEQ ID NO: 2510; SEQ ID NO: 2511; SEQ ID NO: 2512; SEQ ID NO: 2513; SEQ ID NO: 2514; SEQ ID NO: 2515; SEQ ID NO: 2516; SEQ ID NO: 2517; SEQ ID NO: 2518; SEQ ID NO: 2519; SEQ ID NO: 2520; SEQ ID NO: 2521; SEQ ID NO: 2522; SEQ ID NO: 2523; SEQ ID NO: 2524; SEQ ID NO: 2525; SEQ ID NO: 2526; SEQ ID NO: 2527; SEQ ID NO: 2528; SEQ ID NO: 2529; SEQ ID NO: 2530; SEQ ID NO: 2531; SEQ ID NO: 2532; SEQ ID NO: 2533; SEQ ID NO: 2534; SEQ ID NO: 2535; SEQ ID NO: 2536; SEQ ID NO: 2537; SEQ ID NO: 2538; SEQ ID NO: 2539; SEQ ID NO: 2540; SEQ ID NO: 2541; SEQ ID NO: 2542; SEQ ID NO: 2543; SEQ ID NO: 2544; SEQ ID NO: 2545; SEQ ID NO: 2546; SEQ ID NO: 2547; SEQ ID NO: 2548; SEQ ID NO: 2549; SEQ ID NO: 2550; SEQ ID NO: 2551; SEQ ID NO: 2552; SEQ ID NO: 2553; SEQ ID NO: 2554; SEQ ID NO: 2555; SEQ ID NO: 2556; SEQ ID NO: 2557; SEQ ID NO: 2558; SEQ ID NO: 2559; SEQ ID NO: 2560; SEQ ID NO: 2561; SEQ ID NO: 2562; SEQ ID NO: 2563; SEQ ID NO: 2564; SEQ ID NO: 2565; SEQ ID NO: 2566; SEQ ID NO: 2567; SEQ ID NO: 2568; SEQ ID NO: 2569; SEQ ID NO: 2570; SEQ ID NO: 2571; SEQ ID NO: 2572; SEQ ID NO: 2573; SEQ ID NO: 2574; SEQ ID NO: 2575; SEQ ID NO: 2576; SEQ ID NO: 2577; SEQ ID NO: 2578; SEQ ID NO: 2579; SEQ ID NO: 2580; SEQ ID NO: 2581; SEQ ID NO: 2582; SEQ ID NO: 2583; SEQ ID NO: 2584; SEQ ID NO: 2585; SEQ ID NO: 2586; SEQ ID NO: 2587; SEQ ID NO: 2588; SEQ ID NO: 2589; SEQ ID NO: 2590; SEQ ID NO: 2591; SEQ ID NO: 2592; SEQ ID NO: 2593; SEQ ID NO: 2594; SEQ ID NO: 2595; SEQ ID NO: 2596; SEQ ID NO: 2597; SEQ ID NO: 2598; SEQ ID NO: 2599; SEQ ID NO: 2600; SEQ ID NO: 2601; SEQ ID NO: 2602; SEQ ID NO: 2603; SEQ ID NO: 2604; SEQ ID NO: 2605; SEQ ID NO: 2606; SEQ ID NO: 2607; SEQ ID NO: 2608; SEQ ID NO: 2609; SEQ ID NO: 2610; SEQ ID NO: 2611; SEQ ID NO: 2612; SEQ ID NO: 2613; SEQ ID NO: 2614; SEQ ID NO: 2615; SEQ ID NO: 2616; SEQ ID NO: 2617; SEQ ID NO: 2618; SEQ ID NO: 2619; SEQ ID NO: 2620; SEQ ID NO: 2621; SEQ ID NO: 2622; SEQ ID NO: 2623; SEQ ID NO: 2624; SEQ ID NO: 2625; SEQ ID NO: 2626; SEQ ID NO: 2627; SEQ ID NO: 2628; SEQ ID NO: 2629; SEQ ID NO: 2630; SEQ ID NO: 2631; SEQ ID NO: 2632; SEQ ID NO: 2633; SEQ ID NO: 2634; SEQ ID NO: 2635; SEQ ID NO: 2636; SEQ ID NO: 2637; SEQ ID NO: 2638; SEQ ID NO: 2639; SEQ ID NO: 2640; SEQ ID NO: 2641; SEQ ID NO: 2642; SEQ ID NO: 2643; SEQ ID NO: 2644; SEQ ID NO: 2645; SEQ ID NO: 2646; SEQ ID NO: 2647; SEQ ID NO: 2648; SEQ ID NO: 2649; SEQ ID NO: 2650; SEQ ID NO: 2651; SEQ ID NO: 2652; SEQ ID NO: 2653; SEQ ID NO: 2654; SEQ ID NO: 2655; SEQ ID NO: 2656; SEQ ID NO: 2657; SEQ ID NO: 2658; SEQ ID NO: 2659; SEQ ID NO: 2660; SEQ ID NO: 2661; SEQ ID NO: 2662; SEQ ID NO: 2663; SEQ ID NO: 2664; SEQ ID NO: 2665; SEQ ID NO: 2666; SEQ ID NO: 2667; SEQ ID NO: 2668; SEQ ID NO: 2669; SEQ ID NO: 2670; SEQ ID NO: 2671; SEQ ID NO: 2672; SEQ ID NO: 2673; SEQ ID NO: 2674; SEQ ID NO: 2675; SEQ ID NO: 2676; SEQ ID NO: 2677; SEQ ID NO: 2678; SEQ ID NO: 2679; SEQ ID NO: 2680; SEQ ID NO: 2681; SEQ ID NO: 2682; SEQ ID NO: 2683; SEQ ID NO: 2684; SEQ ID NO: 2685; SEQ ID NO: 2686; SEQ ID NO: 2687; SEQ ID NO: 2688; SEQ ID NO: 2689; SEQ ID NO: 2690; SEQ ID NO: 2691; SEQ ID NO: 2692; SEQ ID NO: 2693; SEQ ID NO: 2694; SEQ ID NO: 2695; SEQ ID NO: 2696; SEQ ID NO: 2697; SEQ ID NO: 2698; SEQ ID NO: 2699; SEQ ID NO: 2700; SEQ ID NO: 2701; SEQ ID NO: 2702; SEQ ID NO: 2703; SEQ ID NO: 2704; SEQ ID NO: 2705; SEQ ID NO: 2706; SEQ ID NO: 2707; SEQ ID NO: 2708; SEQ ID NO: 2709; SEQ ID NO: 2710; SEQ ID NO: 2711; SEQ ID NO: 2712; SEQ ID NO: 2713; SEQ ID NO: 2714; SEQ ID NO: 2715; SEQ ID NO: 2716; SEQ ID NO: 2717; SEQ ID NO: 2718; SEQ ID NO: 2719; SEQ ID NO: 2720; SEQ ID NO: 2721; SEQ ID NO: 2722; SEQ ID NO: 2723; SEQ ID NO: 2724; SEQ ID NO: 2725; SEQ ID NO: 2726; SEQ ID NO: 2727; SEQ ID NO: 2728; SEQ ID NO: 2729; SEQ ID NO: 2730; SEQ ID NO: 2731; SEQ ID NO: 2732; SEQ ID NO: 2733; SEQ ID NO: 2734; SEQ ID NO: 2735; SEQ ID NO: 2736; SEQ ID NO: 2737; SEQ ID NO: 2738; SEQ ID NO: 2739; SEQ ID NO: 2740; SEQ ID NO: 2741; SEQ ID NO: 2742; SEQ ID NO: 2743; SEQ ID NO: 2744; SEQ ID NO: 2745; SEQ ID NO: 2746; SEQ ID NO: 2747; SEQ ID NO: 2748; SEQ ID NO: 2749; SEQ ID NO: 2750; SEQ ID NO: 2751; SEQ ID NO: 2752; SEQ ID NO: 2753; SEQ ID NO: 2754; SEQ ID NO: 2755; SEQ ID NO: 2756; SEQ ID NO: 2757; SEQ ID NO: 2758; SEQ ID NO: 2759; SEQ ID NO: 2760; SEQ ID NO: 2761; SEQ ID NO: 2762; SEQ ID NO: 2763; SEQ ID NO: 2764; SEQ ID NO: 2765; SEQ ID NO: 2766; SEQ ID NO: 2767; SEQ ID NO: 2768; SEQ ID NO: 2769; SEQ ID NO: 2770; SEQ ID NO: 2771; SEQ ID NO: 2772; SEQ ID NO: 2773; SEQ ID NO: 2774; SEQ ID NO: 2775; SEQ ID NO: 2776; SEQ ID NO: 2777; SEQ ID NO: 2778; SEQ ID NO: 2779; SEQ ID NO: 2780; SEQ ID NO: 2781; SEQ ID NO: 2782; SEQ ID NO: 2783; SEQ ID NO: 2784; SEQ ID NO: 2785; SEQ ID NO: 2786; SEQ ID NO: 2787; SEQ ID NO: 2788; SEQ ID NO: 2789; SEQ ID NO: 2790; SEQ ID NO: 2791; SEQ ID NO: 2792; SEQ ID NO: 2793; SEQ ID NO: 2794; SEQ ID NO: 2795; SEQ ID NO: 2796; SEQ ID NO: 2797; SEQ ID NO: 2798; SEQ ID NO: 2799; SEQ ID NO: 2800; SEQ ID NO: 2801; SEQ ID NO: 2802; SEQ ID NO: 2803; SEQ ID NO: 2804; SEQ ID NO: 2805; SEQ ID NO: 2806; SEQ ID NO: 2807; SEQ ID NO: 9605; SEQ ID NO: 9606; SEQ ID NO: 9607; SEQ ID NO: 9608.  
     [0156] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in secretion and adhesion or a fragment thereof encoded by a nucleic acid having a sequence selected from the group consisting of SEQ ID NO: 2808; SEQ ID NO: 2809; SEQ ID NO: 2810; SEQ ID NO: 2811; SEQ ID NO: 2812; SEQ ID NO: 2813; SEQ ID NO: 2814; SEQ ID NO: 2815; SEQ ID NO: 2816; SEQ ID NO: 2817; SEQ ID NO: 2818; SEQ ID NO: 2819; SEQ ID NO: 2820; SEQ ID NO: 2821; SEQ ID NO: 2822; SEQ ID NO: 2823; SEQ ID NO: 2824.  
     [0157] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in outer membrane and cell wall biogenesis or a fragment thereof encoded by a nucleic acid having a sequence selected from the group consisting of SEQ ID NO: 2825; SEQ ID NO: 2826; SEQ ID NO: 2827; SEQ ID NO: 2828; SEQ ID NO: 2829; SEQ ID NO: 2830; SEQ ID NO: 2831; SEQ ID NO: 2832; SEQ ID NO: 2833; SEQ ID NO: 2834; SEQ ID NO: 2835; SEQ ID NO: 2836; SEQ ID NO: 2837; SEQ ID NO: 2838; SEQ ID NO: 2839; SEQ ID NO: 2840; SEQ ID NO: 2841; SEQ ID NO: 2842; SEQ ID NO: 2843; SEQ ID NO: 2844; SEQ ID NO: 2845; SEQ ID NO: 2846; SEQ ID NO: 2847; SEQ ID NO: 2848; SEQ ID NO: 2849; SEQ ID NO: 2850; SEQ ID NO: 2851; SEQ ID NO: 2852; SEQ ID NO: 2853; SEQ ID NO: 2854; SEQ ID NO: 2855; SEQ ID NO: 2856; SEQ ID NO: 2857; SEQ ID NO: 2858; SEQ ID NO: 2859; SEQ ID NO: 2860; SEQ ID NO: 2861; SEQ ID NO: 2862; SEQ ID NO: 2863; SEQ ID NO: 2864; SEQ ID NO: 2865; SEQ ID NO: 2866; SEQ ID NO: 2867; SEQ ID NO: 2868; SEQ ID NO: 2869; SEQ ID NO: 2870; SEQ ID NO: 2871; SEQ ID NO: 2872; SEQ ID NO: 2873; SEQ ID NO: 2874; SEQ ID NO: 2875; SEQ ID NO: 2876; SEQ ID NO: 2877; SEQ ID NO: 9609; and SEQ ID NO: 9610.  
     [0158] In another embodiment, the  H. pylori  cytoplasmic polypeptide or a fragment thereof is an  H. pylori  polypeptide involved in protein folding and stabilization or a fragment thereof encoded by a nucleic acid having a sequence selected from the group consisting of SEQ ID NO:2878; SEQ ID NO: 2879; SEQ ID NO: 2880; SEQ ID NO: 2881; SEQ ID NO: 2882; SEQ ID NO: 2883; SEQ ID NO: 2884; SEQ ID NO: 2885; SEQ ID NO: 2886; SEQ ID NO: 2887; SEQ ID NO: 2888; SEQ ID NO: 2889; SEQ ID NO: 2890; SEQ ID NO: 2891; SEQ ID NO: 2892; SEQ ID NO: 2893; SEQ ID NO: 2894; SEQ ID NO: 2895; SEQ ID NO: 2896; SEQ ID NO: 2897; SEQ ID NO: 2898; SEQ ID NO: 2899; SEQ ID NO: 2900; SEQ ID NO: 2901; SEQ ID NO: 2902; SEQ ID NO: 2903; SEQ ID NO: 2904; SEQ ID NO: 2905; SEQ ID NO: 2906; SEQ ID NO: 2907; SEQ ID NO: 2908; SEQ ID NO: 2909; SEQ ID NO: 2910; SEQ ID NO: 2911; SEQ ID NO: 2912; SEQ ID NO: 2913; SEQ ID NO: 2914; SEQ ID NO: 2915; SEQ ID NO: 2916; SEQ ID NO: 2917; SEQ ID NO: 2918; SEQ ID NO: 9611 and SEQ ID NO: 9612.  
     [0159] Particularly preferred is an isolated  H. pylori  secreted polypeptide or a fragment thereof, wherein the polypeptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 8778; SEQ ID NO: 79; SEQ ID NO: 8780; SEQ ID NO: 8781; SEQ ID NO: 8782; SEQ ID NO: 8783; SEQ ID NO: 8784; SEQ ID NO: 8785; SEQ ID NO: 8786; SEQ ID NO: 8787; SEQ ID NO: 8788; SEQ ID NO: 8789; SEQ ID NO: 8790; SEQ ID NO: 8791; SEQ ID NO: 8792; SEQ ID NO: 8793; SEQ ID NO: 8794; SEQ ID NO: 8795; SEQ ID NO: 8796; SEQ ID NO: 8797; SEQ ID NO: 8798; SEQ ID NO: 8799; SEQ ID NO: 8800; SEQ ID NO: 8801; SEQ ID NO: 8802; SEQ ID NO: 8803; SEQ ID NO: 8804; SEQ ID NO: 8805; SEQ ID NO: 8806; SEQ ID NO: 8807; SEQ ID NO: 8808; SEQ ID NO: 8809; SEQ ID NO: 8810; SEQ ID NO: 8811; SEQ ID NO: 8812; SEQ ID NO: 8813; SEQ ID NO: 8814; SEQ ID NO: 8815; SEQ ID NO: 8816; SEQ ID NO: 8817; SEQ ID NO: 8818; SEQ ID NO: 8819; SEQ ID NO: 8820; SEQ ID NO: 8821; SEQ ID NO: 8822; SEQ ID NO: 8823; SEQ ID NO: 8824; SEQ ID NO: 8825; SEQ ID NO: 8826; SEQ ID NO: 8827; SEQ ID NO: 8828; SEQ ID NO: 8829; SEQ ID NO: 8830; SEQ ID NO: 8831; SEQ ID NO: 8832; SEQ ID NO: 8833; SEQ ID NO: 8834; SEQ ID NO: 8835; SEQ ID NO: 8836; SEQ ID NO: 8837; SEQ ID NO: 8838; SEQ ID NO: 8839; SEQ ID NO: 8840; SEQ ID NO: 8841; SEQ ID NO:8842; SEQ ID NO:8843; SEQ ID NO:8844; SEQ ID NO:8845; SEQ ID NO: 8846; SEQ ID NO: 8847; SEQ ID NO: 8848; SEQ ID NO: 8849; SEQ ID NO: 8850; SEQ ID NO: 8851; SEQ ID NO: 8852; SEQ ID NO: 8853; SEQ ID NO: 8854; SEQ ID NO: 8855; SEQ ID NO: 8856; SEQ ID NO: 8857; SEQ ID NO: 8858; SEQ ID NO: 8859; SEQ ID NO: 8860; SEQ ID NO: 8861; SEQ ID NO: 8862; SEQ ID NO: 8863; SEQ ID NO: 8864; SEQ ID NO: 8865; SEQ ID NO: 8866; SEQ ID NO: 8867; SEQ ID NO: 8868; SEQ ID NO: 8869; SEQ ID NO: 8870; SEQ ID NO: 8871; SEQ ID NO: 8872; SEQ ID NO: 8873; SEQ ID NO: 8874; SEQ ID NO: 8875; SEQ ID NO: 8876; SEQ ID NO: 8877; SEQ ID NO: 8878; SEQ ID NO: 8879; SEQ ID NO: 8880; SEQ ID NO: 8881; SEQ ID NO: 8882; SEQ ID NO: 8883; SEQ ID NO: 8884; SEQ ID NO: 8885; SEQ ID NO: 8886; SEQ ID NO: 8887; SEQ ID NO: 8888; SEQ ID NO: 8889; SEQ ID NO: 8890; SEQ ID NO: 8891; SEQ ID NO: 8892; SEQ ID NO: 8893; SEQ ID NO: 8894; SEQ ID NO: 8895; SEQ ID NO: 8896; SEQ ID NO: 8897; SEQ ID NO: 8898; SEQ ID NO: 8899; SEQ ID NO: 8900; SEQ ID NO: 8901; SEQ ID NO: 8902; SEQ ID NO: 8903; SEQ ID NO: 8904; SEQ ID NO: 8905; SEQ ID NO: 8906; SEQ ID NO: 8907; SEQ ID NO: 8908; SEQ ID NO: 8909; SEQ ID NO: 8910; SEQ ID NO: 8911; SEQ ID NO: 8912; SEQ ID NO: 8913; SEQ ID NO: 8914; SEQ ID NO: 8915; SEQ ID NO: 8916; SEQ ID NO: 8917; SEQ ID NO: 8918; SEQ ID NO: 8919; SEQ ID NO: 8920; SEQ ID NO: 8921; SEQ ID NO: 8922; SEQ ID NO: 8923; SEQ ID NO: 8924; SEQ ID NO: 8925; SEQ ID NO: 8926; SEQ ID NO: 8927; SEQ ID NO: 8928; SEQ ID NO: 8929; SEQ ID NO: 8930; SEQ ID NO: 8931; SEQ ID NO: 8932; SEQ ID NO: 8933; SEQ ID NO: 8934; SEQ ID NO: 8935; SEQ ID NO: 8936; SEQ ID NO: 8937; SEQ ID NO: 8938; SEQ ID NO: 8939; SEQ ID NO: 8940; SEQ ID NO: 8941; SEQ ID NO: 8942; SEQ ID NO: 8943; SEQ ID NO: 8944; SEQ ID NO: 8945; SEQ ID NO: 8946; SEQ ID NO: 8947; SEQ ID NO: 8948; SEQ ID NO: 8949; SEQ ID NO: 8950; SEQ ID NO: 8951; SEQ ID NO: 8952; SEQ ID NO: 8953; SEQ ID NO: 8954; SEQ ID NO: 8955; SEQ ID NO: 8956; SEQ ID NO: 8957; SEQ ID NO: 8958; SEQ ID NO: 8959; SEQ ID NO: 8960; SEQ ID NO: 8961; SEQ ID NO: 8962; SEQ ID NO: 8963; SEQ ID NO: 8964; SEQ ID NO: 8965; SEQ ID NO: 8966; SEQ ID NO: 8967; SEQ ID NO: 8968; SEQ ID NO: 8969; SEQ ID NO: 8970; SEQ ID NO: 8971; SEQ ID NO: 8972; SEQ ID NO: 8973; SEQ ID NO: 8974; SEQ ID NO: 8975; SEQ ID NO: 8976; SEQ ID NO: 8977; SEQ ID NO: 8978; SEQ ID NO: 8979; SEQ ID NO: 8980; SEQ ID NO: 8981; SEQ ID NO: 8982; SEQ ID NO: 8983; SEQ ID NO: 8984; SEQ ID NO: 8985; SEQ ID NO: 8986; SEQ ID NO: 8987; SEQ ID NO: 8988; SEQ ID NO: 8989; SEQ ID NO: 8990; SEQ ID NO: 8991; SEQ ID NO: 8992; SEQ ID NO: 8993; SEQ ID NO: 8994; SEQ ID NO: 8995; SEQ ID NO: 8996; SEQ ID NO: 8997; SEQ ID NO: 8998; SEQ ID NO: 8999; SEQ ID NO: 9000; SEQ ID NO: 9001; SEQ ID NO: 9002; SEQ ID NO: 9003; SEQ ID NO: 9004; SEQ ID NO: 9005; SEQ ID NO: 9006; SEQ ID NO: 9007; SEQ ID NO: 9008; SEQ ID NO: 9009; SEQ ID NO: 9010; SEQ ID NO: 9011; SEQ ID NO: 9012; SEQ ID NO: 9013; SEQ ID NO: 9014; SEQ ID NO: 9015; SEQ ID NO: 9016; SEQ ID NO: 9017; SEQ ID NO: 9018; SEQ ID NO: 9019; SEQ ID NO: 9020; SEQ ID NO: 9021; SEQ ID NO: 9022; SEQ ID NO: 9023; SEQ ID NO: 9024; SEQ ID NO: 9025; SEQ ID NO: 9026; SEQ ID NO: 9027; SEQ ID NO: 9028; SEQ ID NO: 9029; SEQ ID NO: 9030; SEQ ID NO: 9031; SEQ ID NO: 9032; SEQ ID NO: 9033; SEQ ID NO: 9034; SEQ ID NO: 9035; SEQ ID NO: 9036; SEQ ID NO: 9037; SEQ ID NO: 9038; SEQ ID NO: 9039; SEQ ID NO: 9040; SEQ ID NO: 9041; SEQ ID NO: 9042; SEQ ID NO: 9043; SEQ ID NO: 9044; SEQ ID NO: 9045; SEQ ID NO: 9046; SEQ ID NO: 9047; SEQ ID NO: 9048; SEQ ID NO: 9049; SEQ ID NO: 9050; SEQ ID NO: 9051; SEQ ID NO: 9052; SEQ ID NO: 9053; SEQ ID NO: 9054; SEQ ID NO: 9055; SEQ ID NO: 9056; SEQ ID NO: 9057; SEQ ID NO: 9058; SEQ ID NO: 9059; SEQ ID NO: 9060; SEQ ID NO: 9061; SEQ ID NO: 9062; SEQ ID NO: 9063; SEQ ID NO: 9064; SEQ ID NO: 9065; SEQ ID NO: 9066; SEQ ID NO: 9067; SEQ ID NO: 9068; SEQ ID NO: 9069; SEQ ID NO: 9070; SEQ ID NO: 9071; SEQ ID NO: 9072; SEQ ID NO: 9073; SEQ ID NO: 9074; SEQ ID NO: 9075; SEQ ID NO: 9076; SEQ ID NO: 9077; SEQ ID NO: 9078; SEQ ID NO: 9079; SEQ ID NO: 9080; SEQ ID NO: 9081; SEQ ID NO: 9082; SEQ ID NO: 9083; SEQ ID NO: 9084; SEQ ID NO: 9085; SEQ ID NO: 9086; SEQ ID NO: 9087; SEQ ID NO: 9088; SEQ ID NO: 9089; SEQ ID NO: 9090; SEQ ID NO: 9091; SEQ ID NO: 9092; SEQ ID NO: 9093; SEQ ID NO: 9094; SEQ ID NO: 9095; SEQ ID NO: 9096; SEQ ID NO: 9097; SEQ ID NO: 9098; SEQ ID NO: 9099; SEQ ID NO: 9100; SEQ ID NO: 9101; SEQ ID NO: 9102; SEQ ID NO: 9103; SEQ ID NO: 9104; SEQ ID NO: 9105; SEQ ID NO: 9106; SEQ ID NO: 9107; SEQ ID NO: 9108; SEQ ID NO: 9109; SEQ ID NO: 9110; SEQ ID NO: 9111; SEQ ID NO: 9112; SEQ ID NO: 9113; SEQ ID NO: 9114; SEQ ID NO: 9115; SEQ ID NO: 9116; SEQ ID NO: 9117; SEQ ID NO: 9118; SEQ ID NO: 9119; SEQ ID NO: 9120; SEQ ID NO: 9121; SEQ ID NO: 9122; SEQ ID NO: 9123; SEQ ID NO: 9124; SEQ ID NO: 9125; SEQ ID NO: 9126; SEQ ID NO: 9127; SEQ ID NO: 9128; SEQ ID NO: 9129; SEQ ID NO: 9130; SEQ ID NO: 9131; SEQ ID NO: 9132; SEQ ID NO: 9133; SEQ ID NO: 9134; SEQ ID NO: 9135; SEQ ID NO: 9136; SEQ ID NO: 9137; SEQ ID NO: 9138; SEQ ID NO: 9139; SEQ ID NO: 9140; SEQ ID NO: 9141; SEQ ID NO: 9142; SEQ ID NO: 9143; SEQ ID NO: 9144; SEQ ID NO: 9145; SEQ ID NO: 9146; SEQ ID NO: 9147; SEQ ID NO: 9148; SEQ ID NO: 9149; SEQ ID NO: 9150; SEQ ID NO: 9734; SEQ ID NO: 9735; SEQ ID NO: 9736 and SEQ ID NO: 9737.  
     [0160] In one embodiment, the  H. pylori  secreted polypeptide or a fragment thereof is a  H. pylori  periplasmic polypeptide or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 8778; SEQ ID NO: 8779 and SEQ ID NO: 8780.  
     [0161] In a further embodiment, the  H. pylori  secreted polypeptide or a fragment thereof is a  H. pylori  polypeptide or a fragment thereof involved in secretion and adhesion having an amino acid sequence selected from the group consisting of SEQ ID NO: 8781; SEQ ID NO: 8782; SEQ ID NO: 8783; SEQ ID NO: 8784; SEQ ID NO: 8785; SEQ ID NO: 8786; SEQ ID NO: 8787; SEQ ID NO: 8788 and SEQ ID NO: 9734.  
     [0162] In a further embodiment, the  H. pylori  secreted polypeptide or a fragment thereof is a  H. pylori  chaperone polypeptide or a fragment thereof having an amino acid sequence selected from the group consisting of SEQ ID NO: 8789; SEQ ID NO: 8790; SEQ ID NO: 8791 and SEQ ID NO: 8792.  
     [0163] Particularly preferred is an isolated  H. pylori  secreted polypeptide or a fragment thereof, wherein the polypeptide is encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NO: 4016; SEQ ID NO: 4017; SEQ ID NO: 4018; SEQ ID NO: 4019; SEQ ID NO: 4020; SEQ ID NO: 4021; SEQ ID NO: 4022; SEQ ID NO: 4023; SEQ ID NO: 4024; SEQ ID NO: 4025; SEQ ID NO: 4026; SEQ ID NO: 4027; SEQ ID NO: 4028; SEQ ID NO: 4029; SEQ ID NO: 4030; SEQ ID NO: 4031; SEQ ID NO: 4032; SEQ ID NO: 4033; SEQ ID NO: 4034; SEQ ID NO: 4035; SEQ ID NO: 4036; SEQ ID NO: 4037; SEQ ID NO: 4038; SEQ ID NO: 4039; SEQ ID NO: 4040; SEQ ID NO: 4041; SEQ ID NO: 4042; SEQ ID NO: 4043; SEQ ID NO: 4044; SEQ ID NO: 4045; SEQ ID NO: 4046; SEQ ID NO: 4047; SEQ ID NO: 4048; SEQ ID NO: 4049; SEQ ID NO: 4050; SEQ ID NO: 4051; SEQ ID NO: 4052; SEQ ID NO: 4053; SEQ ID NO: 4054; SEQ ID NO: 4055; SEQ ID NO: 4056; SEQ ID NO: 4057; SEQ ID NO: 4058; SEQ ID NO: 4059; SEQ ID NO: 4060; SEQ ID NO: 4061; SEQ ID NO: 4062; SEQ ID NO: 4063; SEQ ID NO: 4064; SEQ ID NO: 4065; SEQ ID NO: 4066; SEQ ID NO: 4067; SEQ ID NO: 4068; SEQ ID NO: 4069; SEQ ID NO: 4070; SEQ ID NO: 4071; SEQ ID NO: 4072; SEQ ID NO: 4073; SEQ ID NO: 4074; SEQ ID NO: 4075; SEQ ID NO: 4076; SEQ ID NO: 4077; SEQ ID NO: 4078; SEQ ID NO: 4079; SEQ ID NO: 4080; SEQ ID NO: 4081; SEQ ID NO: 4082; SEQ ID NO: 4083; SEQ ID NO: 4084; SEQ ID NO: 4085; SEQ ID NO: 4086; SEQ ID NO: 4087; SEQ ID NO: 4088; SEQ ID NO: 4089; SEQ ID NO: 4090; SEQ ID NO: 4091; SEQ ID NO: 4092; SEQ ID NO: 4093; SEQ ID NO: 4094; SEQ ID NO: 4095; SEQ ID NO: 4096; SEQ ID NO: 4097; SEQ ID NO: 4098; SEQ ID NO: 4099; SEQ ID NO: 4100; SEQ ID NO: 4101; SEQ ID NO: 4102; SEQ ID NO: 4103; SEQ ID NO: 4104; SEQ ID NO: 4105; SEQ ID NO: 4106; SEQ ID NO: 4107; SEQ ID NO: 4108; SEQ ID NO: 4109; SEQ ID NO: 4110; SEQ ID NO: 4111; SEQ ID NO: 4112; SEQ ID NO: 4113; SEQ ID NO: 4114; SEQ ID NO: 4115; SEQ ID NO: 4116; SEQ ID NO: 4117; SEQ ID NO: 4118; SEQ ID NO: 4119; SEQ ID NO: 4120; SEQ ID NO: 4121; SEQ ID NO: 4122; SEQ ID NO: 4123; SEQ ID NO: 4124; SEQ ID NO: 4125; SEQ ID NO: 4126; SEQ ID NO: 4127; SEQ ID NO: 4128; SEQ ID NO: 4129; SEQ ID NO: 4130; SEQ ID NO: 4131; SEQ ID NO: 4132; SEQ ID NO: 4133; SEQ ID NO: 4134; SEQ ID NO: 4135; SEQ ID NO: 4136; SEQ ID NO: 4137; SEQ ID NO: 4138; SEQ ID NO: 4139; SEQ ID NO: 4140; SEQ ID NO: 4141; SEQ ID NO: 4142; SEQ ID NO: 4143; SEQ ID NO: 4144; SEQ ID NO: 4145; SEQ ID NO: 4146; SEQ ID NO: 4147; SEQ ID NO: 4148; SEQ ID NO: 4149; SEQ ID NO: 4150; SEQ ID NO: 4151; SEQ ID NO: 4152; SEQ ID NO: 4153; SEQ ID NO: 4154; SEQ ID NO: 4155; SEQ ID NO: 4156; SEQ ID NO: 4157; SEQ ID NO: 4158; SEQ ID NO: 4159; SEQ ID NO: 4160; SEQ ID NO: 4161; SEQ ID NO: 4162; SEQ ID NO: 4163; SEQ ID NO: 4164; SEQ ID NO: 4165; SEQ ID NO: 4166; SEQ ID NO: 4167; SEQ ID NO: 4168; SEQ ID NO: 4169; SEQ ID NO: 4170; SEQ ID NO: 4171; SEQ ID NO: 4172; SEQ ID NO: 4173; SEQ ID NO: 4174; SEQ ID NO: 4175; SEQ ID NO: 4176; SEQ ID NO: 4177; SEQ ID NO: 4178; SEQ ID NO: 4179; SEQ ID NO: 4180; SEQ ID NO: 4181; SEQ ID NO: 4182; SEQ ID NO: 4183; SEQ ID NO: 4184; SEQ ID NO: 4185; SEQ ID NO: 4186; SEQ ID NO: 4187; SEQ ID NO: 4188; SEQ ID NO: 4189; SEQ ID NO: 4190; SEQ ID NO: 4191; SEQ ID NO: 4192; SEQ ID NO: 4193; SEQ ID NO: 4194; SEQ ID NO: 4195; SEQ ID NO: 4196; SEQ ID NO: 4197; SEQ ID NO: 4198; SEQ ID NO: 4199; SEQ ID NO: 4200; SEQ ID NO: 4201; SEQ ID NO: 4202; SEQ ID NO: 4203; SEQ ID NO: 4204; SEQ ID NO: 4205; SEQ ID NO: 4206; SEQ ID NO: 4207; SEQ ID NO: 4208; SEQ ID NO: 4209; SEQ ID NO: 4210; SEQ ID NO: 4211; SEQ ID NO: 4212; SEQ ID NO: 4213; SEQ ID NO: 4214; SEQ ID NO: 4215; SEQ ID NO: 4216; SEQ ID NO: 4217; SEQ ID NO: 4218; SEQ ID NO: 4219; SEQ ID NO: 4220; SEQ ID NO: 4221; SEQ ID NO: 4222; SEQ ID NO: 4223; SEQ ID NO: 4224; SEQ ID NO: 4225; SEQ ID NO: 4226; SEQ ID NO: 4227; SEQ ID NO: 4228; SEQ ID NO: 4229; SEQ ID NO: 4230; SEQ ID NO: 4231; SEQ ID NO: 4232; SEQ ID NO: 4233; SEQ ID NO: 4234; SEQ ID NO: 4235; SEQ ID NO: 4236; SEQ ID NO: 4237; SEQ ID NO: 4238; SEQ ID NO: 4239; SEQ ID NO: 4240; SEQ ID NO: 4241; SEQ ID NO: 4242; SEQ ID NO: 4243; SEQ ID NO: 4244; SEQ ID NO: 4245; SEQ ID NO: 4246; SEQ ID NO: 4247; SEQ ID NO: 4248; SEQ ID NO: 4249; SEQ ID NO: 4250; SEQ ID NO: 4251; SEQ ID NO: 4252; SEQ ID NO: 4253; SEQ ID NO: 4254; SEQ ID NO: 4255; SEQ ID NO: 4256; SEQ ID NO: 4257; SEQ ID NO: 4258; SEQ ID NO: 4259; SEQ ID NO: 4260; SEQ ID NO: 4261; SEQ ID NO: 4262; SEQ ID NO: 4263; SEQ ID NO: 4264; SEQ ID NO: 4265; SEQ ID NO: 4266; SEQ ID NO: 4267; SEQ ID NO: 4268; SEQ ID NO: 4269; SEQ ID NO: 4270; SEQ ID NO: 4271; SEQ ID NO: 4272; SEQ ID NO: 4273; SEQ ID NO: 4274; SEQ ID NO: 4275; SEQ ID NO: 4276; SEQ ID NO: 4277; SEQ ID NO: 4278; SEQ ID NO: 4279; SEQ ID NO: 4280; SEQ ID NO: 4281; SEQ ID NO: 4282; SEQ ID NO: 4283; SEQ ID NO: 4284; SEQ ID NO: 4285; SEQ ID NO: 4286; SEQ ID NO: 4287; SEQ ID NO: 4288; SEQ ID NO: 4289; SEQ ID NO: 4290; SEQ ID NO: 4291; SEQ ID NO: 4292; SEQ ID NO: 4293; SEQ ID NO: 4294; SEQ ID NO: 4295; SEQ ID NO: 4296; SEQ ID NO: 4297; SEQ ID NO: 4298; SEQ ID NO: 4299; SEQ ID NO: 4300; SEQ ID NO: 4301; SEQ ID NO: 4302; SEQ ID NO: 4303; SEQ ID NO: 4304; SEQ ID NO: 4305; SEQ ID NO: 4306; SEQ ID NO: 4307; SEQ ID NO: 4308; SEQ ID NO: 4309; SEQ ID NO: 4310; SEQ ID NO: 4311; SEQ ID NO: 4312; SEQ ID NO: 4313; SEQ ID NO: 4314; SEQ ID NO: 4315; SEQ ID NO: 4316; SEQ ID NO: 4317; SEQ ID NO: 4318; SEQ ID NO: 4319; SEQ ID NO: 4320; SEQ ID NO: 4321; SEQ ID NO: 4322; SEQ ID NO: 4323; SEQ ID NO: 4324; SEQ ID NO: 4325; SEQ ID NO: 4326; SEQ ID NO: 4327; SEQ ID NO: 4328; SEQ ID NO: 4329; SEQ ID NO: 4330; SEQ ID NO: 4331; SEQ ID NO: 4332; SEQ ID NO: 4333; SEQ ID NO: 4334; SEQ ID NO: 4335; SEQ ID NO: 4336; SEQ ID NO: 4337; SEQ ID NO: 4388; SEQ ID NO: 9622; SEQ ID NO: 9623; SEQ ID NO: 9624 and SEQ ID NO: 9625.  
     [0164] In one embodiment, the  H. pylori  secreted polypeptide or a fragment thereof is a  H. pylori  periplasmic polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 4016; SEQ ID NO: 4017 and SEQ ID NO: 4018.  
     [0165] In a further embodiment, the  H. pylori  secreted polypeptide or a fragment thereof is a  H. pylori  polypeptide or a fragment thereof involved in secretion and adhesion encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NO: 4019; SEQ ID NO: 4020; SEQ ID NO: 4021; SEQ ID NO: 4022; SEQ ID NO: 4023; SEQ ID NO: 4024; SEQ ID NO: 4025; SEQ ID NO: 4026 and SEQ ID NO: 9622.  
     [0166] In a further embodiment, the  H. pylori  secreted polypeptide or a fragment thereof is a  H. pylori  chaperone polypeptide or a fragment thereof encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 4027; SEQ ID NO: 4028; SEQ ID NO: 4029 and SEQ ID NO: 4030.  
     [0167] Particularly preferred is an isolated  H. pylori  cellular polypeptide or a fragment thereof, wherein the polypeptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 9151; SEQ ID NO: 9152; SEQ ID NO: 9153; SEQ ID NO: 9154; SEQ ID NO: 9155; SEQ ID NO: 9156; SEQ ID NO: 9157; SEQ ID NO: 9158; SEQ ID NO: 9159; SEQ ID NO: 9160; SEQ ID NO: 9161; SEQ ID NO: 9162; SEQ ID NO: 9163; SEQ ID NO: 9164; SEQ ID NO: 9165; SEQ ID NO: 9166; SEQ ID NO: 9167; SEQ ID NO: 9168; SEQ ID NO: 9169; SEQ ID NO: 9170; SEQ ID NO: 9171; SEQ ID NO: 9172; SEQ ID NO: 9173; SEQ ID NO: 9174; SEQ ID NO: 9175; SEQ ID NO: 9176; SEQ ID NO: 9177; SEQ-ID NO: 9178; SEQ ID NO: 9179; SEQ ID NO: 9180; SEQ ID NO: 9181; SEQ ID NO: 9182; SEQ ID NO: 9183; SEQ ID NO: 9184; SEQ ID NO: 9185; SEQ ID NO: 9186; SEQ ID NO: 9187; SEQ ID NO: 9188; SEQ ID NO: 9189; SEQ ID NO: 9190; SEQ ID NO: 9191; SEQ ID NO: 9192; SEQ ID NO: 9193; SEQ ID NO: 9194; SEQ ID NO: 9195; SEQ ID NO: 9196; SEQ ID NO: 9197; SEQ ID NO: 9198; SEQ ID NO: 9199; SEQ ID NO: 9200; SEQ ID NO: 9201; SEQ ID NO: 9202; SEQ ID NO: 9203; SEQ ID NO: 9204; SEQ ID NO: 9205; SEQ ID NO: 9206; SEQ ID NO: 9207; SEQ ID NO: 9208; SEQ ID NO: 9209; SEQ ID NO: 9210; SEQ ID NO: 9211; SEQ ID NO: 9212; SEQ ID NO: 9213; SEQ ID NO: 9214; SEQ ID NO: 9215; SEQ ID NO: 9216; SEQ ID NO: 9217; SEQ ID NO: 9218; SEQ ID NO: 9219; SEQ ID NO: 9220; SEQ ID NO: 9221; SEQ ID NO: 9222; SEQ ID NO: 9223; SEQ ID NO: 9224; SEQ ID NO: 9225; SEQ ID NO: 9226; SEQ ID NO: 9227; SEQ ID NO: 9228; SEQ ID NO: 9229; SEQ ID NO: 9230; SEQ ID NO: 9231; SEQ ID NO: 9232; SEQ ID NO: 9233; SEQ ID NO: 9234; SEQ ID NO: 9235; SEQ ID NO: 9236; SEQ ID NO: 9237; SEQ ID NO: 9238; SEQ ID NO: 9239; SEQ ID NO: 9240; SEQ ID NO: 9241; SEQ ID NO: 9242; SEQ ID NO: 9243; SEQ ID NO: 9244; SEQ ID NO: 9245; SEQ ID NO: 9246; SEQ ID NO: 9247; SEQ ID NO: 9248; SEQ ID NO: 9249; SEQ ID NO: 9250; SEQ ID NO: 9251; SEQ ID NO: 9252; SEQ ID NO:9253; SEQ ID NO: 9254; SEQ ID NO: 9255; SEQ ID NO: 9256; SEQ ID NO: 9257; SEQ ID NO: 9258; SEQ ID NO: 9259; SEQ ID NO: 9260; SEQ ID NO: 9261; SEQ ID NO: 9262; SEQ ID NO: 9263; SEQ ID NO: 9264; SEQ ID NO: 9265; SEQ ID NO: 9266; SEQ ID NO: 9267; SEQ ID NO: 9268; SEQ ID NO: 9269; SEQ ID NO: 9270; SEQ ID NO: 9271; SEQ ID NO: 9272; SEQ ID NO: 9273; SEQ ID NO: 9274; SEQ ID NO: 9275; SEQ ID NO: 9276; SEQ ID NO: 9277; SEQ ID NO: 9278; SEQ ID NO: 9279; SEQ ID NO: 9280; SEQ ID NO: 9281; SEQ ID NO: 9282; SEQ ID NO: 9283; SEQ ID NO: 9284; SEQ ID NO: 9285; SEQ ID NO: 9286; SEQ ID NO: 9287; SEQ ID NO: 9288; SEQ ID NO: 9289; SEQ ID NO: 9290; SEQ ID NO: 9291; SEQ ID NO: 9292; SEQ ID NO: 9293; SEQ ID NO: 9294; SEQ ID NO: 9295; SEQ ID NO: 9296; SEQ ID NO: 9297; SEQ ID NO: 9298; SEQ ID NO: 9299; SEQ ID NO: 9300; SEQ ID NO: 9301; SEQ ID NO: 9302; SEQ ID NO: 9303; SEQ ID NO: 9304; SEQ ID NO: 9305; SEQ ID NO: 9306; SEQ ID NO: 9307; SEQ ID NO: 9308; SEQ ID NO: 9309; SEQ ID NO: 9310; SEQ ID NO: 9311; SEQ ID NO: 9312; SEQ ID NO: 9313; SEQ ID NO: 9314; SEQ ID NO: 9315; SEQ ID NO: 9316; SEQ ID NO: 9317; SEQ ID NO: 9318; SEQ ID NO: 9319; SEQ ID NO: 9320; SEQ ID NO: 9321; SEQ ID NO: 9322; SEQ ID NO: 9323; SEQ ID NO: 9324; SEQ ID NO: 9325; SEQ ID NO: 9326; SEQ ID NO: 9327; SEQ ID NO: 9328; SEQ ID NO: 9329; SEQ ID NO: 9330; SEQ ID NO: 9331; SEQ ID NO: 9332; SEQ ID NO: 9333; SEQ ID NO: 9334; SEQ ID NO: 9335; SEQ ID NO: 9336; SEQ ID NO: 9337; SEQ ID NO: 9338; SEQ ID NO: 9339; SEQ ID NO: 9340; SEQ ID NO: 9341; SEQ ID NO: 9342; SEQ ID NO: 9343; SEQ ID NO: 9344; SEQ ID NO: 9345; SEQ ID NO: 9346; SEQ ID NO: 9347; SEQ ID NO: 9348; SEQ ID NO: 9349; SEQ ID NO: 9350; SEQ ID NO: 9351; SEQ ID NO: 9352; SEQ ID NO: 9353; SEQ ID NO: 9354; SEQ ID NO: 9355; SEQ ID NO: 9356; SEQ ID NO: 9357; SEQ ID NO: 9358; SEQ ID NO: 9359; SEQ ID NO: 9360; SEQ ID NO: 9361; SEQ ID NO: 9362; SEQ ID NO: 9363; SEQ ID NO: 9364; SEQ ID NO: 9365; SEQ ID NO: 9366; SEQ ID NO: 9367; SEQ ID NO: 9368; SEQ ID NO: 9369; SEQ ID NO: 9370; SEQ ID NO: 9371; SEQ ID NO: 9372; SEQ ID NO: 9373; SEQ ID NO: 9374; SEQ ID NO: 9375; SEQ ID NO: 9376; SEQ ID NO: 9377; SEQ ID NO: 9378; SEQ ID NO: 9379; SEQ ID NO: 9380; SEQ ID NO: 9381; SEQ ID NO: 9382; SEQ ID NO: 9383; SEQ ID NO: 9384; SEQ ID NO: 9385; SEQ ID NO: 9386; SEQ ID NO: 9387; SEQ ID NO: 9388; SEQ ID NO: 9389; SEQ ID NO: 9390; SEQ ID NO: 9391; SEQ ID NO: 9392; SEQ ID NO: 9393; SEQ ID NO: 9394; SEQ ID NO: 9395; SEQ ID NO: 9396; SEQ ID NO: 9397; SEQ ID NO: 9398; SEQ ID NO: 9399; SEQ ID NO: 9400; SEQ ID NO: 9401; SEQ ID NO: 9402; SEQ ID NO: 9403; SEQ ID NO: 9404; SEQ ID NO: 9405; SEQ ID NO: 9406; SEQ ID NO: 9407; SEQ ID NO: 9408; SEQ ID NO: 9409; SEQ ID NO: 9410; SEQ ID NO: 9411; SEQ ID NO: 9412; SEQ ID NO: 9413; SEQ ID NO: 9414; SEQ ID NO: 9415; SEQ ID NO: 9416; SEQ ID NO: 9417; SEQ ID NO: 9418; SEQ ID NO: 9419; SEQ ID NO: 9420; SEQ ID NO: 9421; SEQ ID NO: 9422; SEQ ID NO: 9423; SEQ ID NO: 9424; SEQ ID NO: 9425; SEQ ID NO: 9426; SEQ ID NO: 9427; SEQ ID NO: 9428; SEQ ID NO: 9429; SEQ ID NO: 9430; SEQ ID NO: 9431; SEQ ID NO: 9432; SEQ ID NO: 9433; SEQ ID NO: 9434; SEQ ID NO: 9435; SEQ ID NO: 9436; SEQ ID NO: 9437; SEQ ID NO: 9438; SEQ ID NO: 9439; SEQ ID NO: 9440; SEQ ID NO: 9441; SEQ ID NO: 9442; SEQ ID NO: 9443; SEQ ID NO: 9444; SEQ ID NO: 9445; SEQ ID NO: 9446; SEQ ID NO: 9447; SEQ ID NO: 9448; SEQ ID NO: 9449; SEQ ID NO: 9450; SEQ ID NO: 9451; SEQ ID NO: 9452; SEQ ID NO: 9453; SEQ ID NO: 9454; SEQ ID NO: 9455; SEQ ID NO: 9456; SEQ ID NO: 9457; SEQ ID NO: 9458; SEQ ID NO: 9459; SEQ ID NO: 9460; SEQ ID NO: 9461; SEQ ID NO: 9462; SEQ ID NO: 9463; SEQ ID NO: 9464; SEQ ID NO: 9465; SEQ ID NO: 9466; SEQ ID NO: 9467; SEQ ID NO: 9738; SEQ ID NO: 9739; SEQ ID NO: 9740; SEQ ID NO: 9741; SEQ ID NO: 9742; SEQ ID NO: 9743; SEQ ID NO: 9744; SEQ ID NO: 9745; SEQ ID NO: 9746; SEQ ID NO: 9747 and SEQ ID NO: 9748.  
     [0168] Particularly preferred is an isolated  H. pylori  cellular polypeptide or a fragment thereof, wherein the polypeptide is encoded by a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 4389; SEQ ID NO: 4390; SEQ ID NO: 4391; SEQ ID NO: 4392; SEQ ID NO: 4393; SEQ ID NO: 4394; SEQ ID NO: 4395; SEQ ID NO: 4396; SEQ ID NO: 4397; SEQ ID NO: 4398; SEQ ID NO: 4399; SEQ ID NO: 4400; SEQ ID NO: 4401; SEQ ID NO: 4402; SEQ ID NO: 4403; SEQ. ID NO: 4404; SEQ ID NO: 4405; SEQ ID NO: 4406; SEQ ID NO: 4407; SEQ ID NO: 4408; SEQ ID NO: 4409; SEQ ID NO: 4410; SEQ ID NO: 4411; SEQ ID NO: 4412; SEQ ID NO:4413; SEQ ID NO:4414; SEQ ID NO:4415; SEQ ID NO:4416; SEQ ID NO: 4417; SEQ ID NO: 4418; SEQ ID NO: 4419; SEQ ID NO: 4420; SEQ ID NO: 4421; SEQ ID NO: 4422; SEQ ID NO: 4423; SEQ ID NO: 4424; SEQ ID NO: 4425; SEQ ID NO: 4426; SEQ ID NO: 4427; SEQ ID NO: 4428; SEQ ID NO: 4429; SEQ ID NO: 4430; SEQ ID NO: 4431; SEQ ID NO: 4432; SEQ ID NO: 4433; SEQ ID NO: 4434; SEQ ID NO: 4435; SEQ ID NO: 4436; SEQ ID NO: 4437; SEQ ID NO: 4438; SEQ ID NO: 4439; SEQ ID NO: 4440; SEQ ID NO: 4441; SEQ ID NO: 4442; SEQ ID NO: 4443; SEQ ID NO: 4444; SEQ ID NO: 4445; SEQ ID NO: 4446; SEQ ID NO: 4447; SEQ ID NO: 4448; SEQ ID NO: 4449; SEQ ID NO: 4450; SEQ ID NO: 4451; SEQ ID NO: 4452; SEQ ID NO: 4453; SEQ ID NO: 4454; SEQ ID NO: 4455; SEQ ID NO: 4456; SEQ ID NO: 4457; SEQ ID NO: 4458; SEQ ID NO: 4459; SEQ ID NO: 4460; SEQ ID NO: 4461; SEQ ID NO: 4462; SEQ ID NO: 4463; SEQ ID NO: 4464; SEQ ID NO: 4465; SEQ ID NO: 4466; SEQ ID NO: 4467; SEQ ID NO: 4468; SEQ ID NO: 4469; SEQ ID NO: 4470; SEQ ID NO: 4471; SEQ ID NO: 4472; SEQ ID NO: 4473; SEQ ID NO: 4474; SEQ ID NO: 4475; SEQ ID NO: 4476; SEQ ID NO: 4477; SEQ ID NO:4478; SEQ ID NO:4479; SEQ ID NO:4480; SEQ ID NO: 4481; SEQ ID NO: 4482; SEQ ID NO: 4483; SEQ ID NO: 4484; SEQ ID NO: 4485; SEQ ID NO: 4486; SEQ ID NO: 4487; SEQ ID NO: 4488; SEQ ID NO: 4489; SEQ ID NO: 4490; SEQ ID NO: 4491; SEQ ID NO: 4492; SEQ ID NO: 4493; SEQ ID NO: 4494; SEQ ID NO: 4495; SEQ ID NO: 4496; SEQ ID NO: 4497; SEQ ID NO: 4498; SEQ ID NO: 4499; SEQ ID NO: 4500; SEQ ID NO: 4501; SEQ ID NO: 4502; SEQ ID NO: 4503; SEQ ID NO: 4504; SEQ ID NO: 4505; SEQ ID NO: 4506; SEQ ID NO: 4507; SEQ ID NO: 4508; SEQ ID NO: 4509; SEQ ID NO: 4510; SEQ ID NO: 4511; SEQ ID NO: 4512; SEQ ID NO: 4513; SEQ ID NO: 4514; SEQ ID NO: 4515; SEQ ID NO: 4516; SEQ ID NO: 4517; SEQ ID NO: 4518; SEQ ID NO: 4519; SEQ ID NO: 4520; SEQ ID NO: 4521; SEQ ID NO: 4522; SEQ ID NO: 4523; SEQ ID NO: 4524; SEQ ID NO: 4525; SEQ ID NO: 4526; SEQ ID NO: 4527; SEQ ID NO: 4528; SEQ ID NO: 4529; SEQ ID NO: 4530; SEQ ID NO: 4531; SEQ ID NO: 4532; SEQ ID NO: 4533; SEQ ID NO: 4534; SEQ ID NO: 4535; SEQ ID NO: 4536; SEQ ID NO: 4537; SEQ ID NO: 4538; SEQ ID NO: 4539; SEQ ID NO: 4540; SEQ ID NO: 4541; SEQ ID NO: 4542; SEQ ID NO: 4543; SEQ ID NO: 4544; SEQ ID NO: 4545; SEQ ID NO: 4546; SEQ ID NO: 4547; SEQ ID NO: 4548; SEQ ID NO: 4549; SEQ ID NO: 4550; SEQ ID NO: 4551; SEQ ID NO: 4552; SEQ ID NO: 4553; SEQ ID NO: 4554; SEQ ID NO: 4555; SEQ ID NO: 4556; SEQ ID NO: 4557; SEQ ID NO: 4558; SEQ ID NO: 4559; SEQ ID NO: 4560; SEQ ID NO: 4561; SEQ ID NO: 4562; SEQ ID NO: 4563; SEQ ID NO: 4564; SEQ ID NO: 4565; SEQ ID NO: 4566; SEQ ID NO: 4567; SEQ ID NO: 4568; SEQ ID NO: 4569; SEQ ID NO: 4570; SEQ ID NO: 4571; SEQ ID NO: 4572; SEQ ID NO: 4573; SEQ ID NO: 4574; SEQ ID NO: 4575; SEQ ID NO: 4576; SEQ ID NO: 4577; SEQ ID NO: 4578; SEQ ID NO: 4579; SEQ ID NO: 4580; SEQ ID NO: 4581; SEQ ID NO: 4582; SEQ ID NO: 4583; SEQ ID NO: 4584; SEQ ID NO: 4585; SEQ ID NO: 4586; SEQ ID NO: 4587; SEQ ID NO: 4588; SEQ ID NO: 4589; SEQ ID NO: 4590; SEQ ID NO: 4591; SEQ ID NO: 4592; SEQ ID NO: 4593; SEQ ID NO: 4594; SEQ ID NO: 4595; SEQ ID NO: 4596; SEQ ID NO: 4597; SEQ ID NO: 4598; SEQ ID NO: 4599; SEQ ID NO: 4600; SEQ ID NO: 4601; SEQ ID NO: 4602; SEQ ID NO: 4603; SEQ ID NO: 4604; SEQ ID NO: 4605; SEQ ID NO: 4606; SEQ ID NO: 4607; SEQ ID NO: 4608; SEQ ID NO: 4609; SEQ ID NO: 4610; SEQ ID NO: 4611; SEQ ID NO: 4612; SEQ ID NO: 4613; SEQ ID NO: 4614; SEQ ID NO: 4615; SEQ ID NO: 4616; SEQ ID NO: 4617; SEQ ID NO: 4618; SEQ ID NO: 4619; SEQ ID NO: 4620; SEQ ID NO: 4621; SEQ ID NO: 4622; SEQ ID NO: 4623; SEQ ID NO: 4624; SEQ ID NO: 4625; SEQ ID NO: 4626; SEQ ID NO: 4627; SEQ ID NO: 4628; SEQ ID NO: 4629; SEQ ID NO: 4630; SEQ ID NO: 4631; SEQ ID NO: 4632; SEQ ID NO: 4633; SEQ ID NO: 4634; SEQ ID NO: 4635; SEQ ID NO: 4636; SEQ ID NO: 4637; SEQ ID NO: 4638; SEQ ID NO: 4639; SEQ ID NO: 4640; SEQ ID NO: 4641; SEQ ID NO: 4642; SEQ ID NO: 4643; SEQ ID NO: 4644; SEQ ID NO: 4645; SEQ ID NO: 4646; SEQ ID NO: 4647; SEQ ID NO: 4648; SEQ ID NO: 4649; SEQ ID NO: 4650; SEQ ID NO: 4651; SEQ ID NO: 4652; SEQ ID NO: 4653; SEQ ID NO: 4654; SEQ ID NO: 4655; SEQ ID NO: 4656; SEQ ID NO: 4657; SEQ ID NO: 4658; SEQ ID NO: 4659; SEQ ID NO: 4660; SEQ ID NO: 4661; SEQ ID NO: 4662; SEQ ID NO: 4663; SEQ ID NO: 4664; SEQ ID NO: 4665; SEQ ID NO: 4666; SEQ ID NO: 4667; SEQ ID NO: 4668; SEQ ID NO: 4669; SEQ ID NO: 4670; SEQ ID NO: 4671; SEQ ID NO: 4672; SEQ ID NO: 4673; SEQ ID NO: 4674; SEQ ID NO: 4675; SEQ ID NO: 4676; SEQ ID NO: 4677; SEQ ID NO: 4678; SEQ ID NO: 4679; SEQ ID NO: 4680; SEQ ID NO: 4681; SEQ ID NO: 4682; SEQ ID NO: 4683; SEQ ID NO: 4684; SEQ ID NO: 4685; SEQ ID NO: 4686; SEQ ID NO: 4687; SEQ ID NO: 4688; SEQ ID NO: 4689; SEQ ID NO: 4690; SEQ ID NO: 4691; SEQ ID NO: 4692; SEQ ID NO: 4693; SEQ ID NO: 4694; SEQ ID NO: 4695; SEQ ID NO: 4696; SEQ ID NO: 4697; SEQ ID NO: 4698; SEQ ID NO: 4699; SEQ ID NO: 4700; SEQ ID NO: 4701; SEQ ID NO: 4702; SEQ ID NO: 4703; SEQ ID NO: 4704; SEQ ID NO: 4705; SEQ ID NO: 9626; SEQ ID NO: 9627; SEQ ID NO: 9628; SEQ ID NO: 9629; SEQ ID NO: 9630; SEQ ID NO: 9631; SEQ ID NO: 9632; SEQ ID NO: 9633; SEQ ID NO: 9634; SEQ ID NO: 9635 and SEQ ID NO: 9636.  
     [0169] Particularly preferred is an isolated  H. pylori  membrane associated polypeptide or a fragment thereof, wherein the polypeptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 9468; SEQ ID NO: 9469; SEQ ID NO: 9470; SEQ ID NO: 9471; SEQ ID NO: 9472; SEQ ID NO: 9473; SEQ ID NO: 9474; SEQ ID NO: 9475; SEQ ID NO: 9476; SEQ ID NO: 9477; SEQ ID NO: 9478; SEQ ID NO: 9479; SEQ ID NO: 9480; SEQ ID NO: 9481; SEQ ID NO: 9482; SEQ ID NO: 9483; SEQ ID NO: 9484; SEQ ID NO: 9485; SEQ ID NO: 9486; SEQ ID NO: 9487; SEQ ID NO: 9488; SEQ ID NO: 9489; SEQ ID NO: 9490; SEQ ID NO: 9491; SEQ ID NO: 9492; SEQ ID NO: 9493; SEQ ID NO: 9494; SEQ ID NO: 9495; SEQ ID NO: 9496; SEQ ID NO: 9497; SEQ ID NO: 9498; SEQ ID NO: 9499; SEQ ID NO: 9500; SEQ ID NO: 9501; SEQ ID NO: 9502; SEQ ID NO: 9503; SEQ ID NO: 9504; SEQ ID NO: 9505; SEQ ID NO: 9506; SEQ ID NO: 9507; SEQ ID NO: 9508; SEQ ID NO: 9509; SEQ ID NO: 9510; SEQ ID NO: 9511; SEQ ID NO: 9512; SEQ ID NO: 9513; SEQ ID NO: 9514; SEQ ID NO: 9515; SEQ ID NO: 9516; SEQ ID NO: 9517; SEQ ID NO: 9518; SEQ ID NO: 9519; SEQ ID NO: 9520; SEQ ID NO: 9521; SEQ ID NO: 9522; SEQ ID NO: 9523 and SEQ ID NO: 9524.  
     [0170] In another aspect, the invention features a chimeric  H. pylori  polypeptide comprising at least two  H. pylori  polypeptides or fragments thereof, wherein the polypeptides are encoded by nucleic acid sequences selected from the group consisting of SEQ ID NO:1-SEQ ID NO:4762 and SEQ ID NO: 9525-SEQ ID NO: 9636.  
     [0171] In another aspect, the invention features a chimeric  H. pylori  polypeptide comprising at least two  H. pylori  polypeptides or fragments thereof, wherein the polypeptides are selected from the group consisting of SEQ ID NO: 4763-SEQ ID NO: 9524 and SEQ ID NO: 9637-SEQ ID NO: 9748.  
     [0172] In another aspect, the invention features a fusion protein comprising an  H. pylori  polypeptide which comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4763-SEQ ID NO: 9524 and SEQ ID NO: 9637-SEQ ID NO: 9748 operatively linked to a non- H. pylori  polypeptide.  
     [0173] In another aspect, the invention features a vaccine formulation for prophylactic or therapeutic treatment of an  H. pylori  infection comprising an effective amount of at least one isolated nucleic acid of the invention.  
     [0174] Preferably, the invention features a vaccine formulation for prophylactic or therapeutic treatment of an  H. pylori  infection comprising an effective amount of at least one isolated nucleic acid encoding an  H. pylori  outer membrane polypeptide or a fragment thereof, which nucleic acid is selected from the group consisting of SEQ ID NO: 212, SEQ ID NO: 254, SEQ ID NO: 205, SEQ ID NO: 329, SEQ ID NO: 384, SEQ ID NO: 377 and SEQ ID NO: 289.  
     [0175] Preferably, the invention features a vaccine formulation for prophylactic or therapeutic treatment of an  H. pylori  infection comprising an effective amount of at least one isolated nucleic acid encoding an  H. pylori  cell envelope polypeptide or a fragment thereof, which nucleic acid is selected from the group consisting of SEQ ID NO: 469, SEQ ID NO: 89, SEQ ID NO: 4286, SEQ ID NO: 419, SEQ ID NO: 9618 and SEQ ID NO: 3253.  
     [0176] In another aspect, the invention features a vaccine formulation for prophylactic or therapeutic treatment of an  H. pylori  infection comprising an effective amount of at least one  H. pylori  polypeptide of the invention.  
     [0177] Preferably, the invention features a vaccine formulation for prophylactic or therapeutic treatment of an  H. pylori  infection comprising an effective amount of at least one  H. pylori  outer membrane polypeptide or a fragment thereof, which polypeptide is selected from the group consisting of SEQ ID NO: 4974, SEQ ID NO: 5016, SEQ ID NO: 4967, SEQ ID NO: 5091, SEQ ID NO: 5146, SEQ ID NO: 5139 and SEQ ID NO: 5051.  
     [0178] Preferably, the invention features a vaccine formulation for prophylactic or therapeutic treatment of an  H. pylori  infection comprising an effective amount of at least one  H. pylori  cell envelope polypeptide or a fragment thereof, which polypeptide is selected from the group consisting of SEQ ID NO: 5231, SEQ ID NO: 4851, SEQ ID NO: 9048, SEQ ID NO: 5181, SEQ ID NO: 9730 and SEQ ID NO: 8015.  
     [0179] Preferably, the vaccine formulation of the invention further includes a pharmaceutically acceptable carrier. In one embodiment, the pharmaceutically acceptable carrier includes an adjuvant. In another embodiment, the pharmaceutically acceptable carrier includes a delivery system, e.g., a live vector, e.g., a bacteria or a virus. In another embodiment, the pharmaceutically acceptable carrier includes both an adjuvant and a delivery system.  
     [0180] In another aspect, the invention features a method of treating or reducing a risk of  H. pylori  infection in a subject. The method includes administering to a subject a vaccine formulation of the invention, such that treatment or reduction of risk of  H. pylori  infection occurs.  
     [0181] In another aspect, the invention features a method of producing a vaccine formulation of the invention. The method includes combining at least one isolated  H. pylori  polypeptide or a fragment thereof selected from the group consisting of SEQ ID NO: 4763-SEQ ID NO: 9524 and SEQ ID NO: 9637-SEQ ID NO: 9748 with a pharmaceutically acceptable carrier to thereby form a vaccine formulation.  
     [0182] In another aspect, the invention features a method of producing a vaccine formulation of the invention. The method includes culturing a cell under conditions that permit expression of an  H. pylori  polypeptide or a fragment thereof selected from the group consisting of SEQ ID NO: 4763-SEQ ID NO: 9524 and SEQ ID NO: 9637-SEQ ID NO: 9748 isolating the  H. pylori  polypeptide from the cell; and combining at least one isolated  H. pylori  polypeptide or a fragment thereof with a pharmaceutically acceptable carrier to thereby form a vaccine formulation.  
     [0183] In another aspect, the invention pertains to any individual  H. pylori  polypeptide member or nucleic acid encoding such a member from the above-identified groups of  H. pylori  polypeptides.  
     [0184] In another aspect, the invention features nucleic acids capable of binding mRNA of  H. pylori . Such nucleic acid is capable of acting as antisense nucleic acid to control the translation of mRNA of  H. pylori . A further aspect features a nucleic acid which is capable of binding specifically to an  H. pylori  nucleic acid. These nucleic acids are also referred to herein as complements and have utility as probes and as capture reagents.  
     [0185] In another aspect, the invention features an expression system comprising an open reading frame corresponding to  H. pylori  nucleic acid. The nucleic acid further comprises a control sequence compatible with an intended host. The expression system is useful for making polypeptides corresponding to  H. pylori  nucleic acid.  
     [0186] In another aspect, the invention features a cell transformed with the expression system to produce  H. pylori  polypeptides.  
     [0187] In another aspect, the invention features a method of generating antibodies against  H. pylori  polypeptides which are capable of binding specifically to  H. pylori  polypeptides. Such antibodies have utility as reagents for immunoassays to evaluate the abundance and distribution of  H. pylori -specific antigens.  
     [0188] In another aspect, the invention features a method of generating vaccines for immunizing an individual against  H. pylori . The vaccination method includes: immunizing a subject with at least one  H. pylori  polypeptide according to the present invention, e.g., a surface or secreted polypeptide, or active portion thereof, and a pharmaceutically acceptable carrier. Such vaccines have therapeutic and/or prophylactic utilities.  
     [0189] In another aspect, the invention provides a method for generating a vaccine comprising a modified immunogenic  H. pylori  polypeptide, e.g., a surface or secreted polypeptide, or active portion thereof, and a pharmacologically acceptable carrier.  
     [0190] In another aspect, the invention features a method of evaluating a compound, e.g. a polypeptide, e.g., a fragment of a host cell polypeptide, for the ability to bind an  H. pylori  polypeptide. The method includes: contacting the candidate compound with an  H. pylori  polypeptide and determining if the compound binds or otherwise interacts with an  H. pylori  polypeptide. Compounds which bind  H. pylori  are candidates as activators or inhibitors of the bacterial life cycle. These assays can be performed in vitro or in vivo.  
     [0191] In another aspect, the invention features a method of evaluating a compound, e.g. a polypeptide, e.g., a fragment of a host cell polypeptide, for the ability to bind an  H. pylori  nucleic acid, e.g., DNA or RNA. The method includes: contacting the candidate compound with an  H. pylori  nucleic acid and determining if the compound binds or otherwise interacts with an  H. pylori  polypeptide. Compounds which bind  H. pylori  are candidates as activators or inhibitors of the bacterial life cycle. These assays can be performed in vitro or in vivo.  
     [0192] The invention features  H. pylori  polypeptides, preferably a substantially pure preparation of an  H. pylori  polypeptide, or a recombinant  H. pylori  polypeptide. In preferred embodiments: the polypeptide has biological activity; the polypeptide has an amino acid sequence at least 60%, 70%, 80%, 90%, 95%, 98%, or 99% identical or homologous to an amino acid sequence of the invention contained in the Sequence Listing, preferably it has about 65% sequence identity with an amino acid sequence of the invention contained in the Sequence Listing, and most preferably it has about 92% to about 99% sequence identity with an amino acid sequence of the invention contained in the Sequence Listing; the polypeptide has an amino acid sequence essentially the same as an amino acid sequence of the invention contained in the Sequence Listing; the polypeptide is at least 5, 10, 20, 50, 100, or 150 amino acid residues in length; the polypeptide includes at least 5, preferably at least 10, more preferably at least 20, more preferably at least 50, 100, or 150 contiguous amino acid residues of the invention contained in the Sequence Listing. In yet another preferred embodiment, the amino acid sequence which differs in sequence identity by about 7% to about 8% from the  H. pylori  amino acid sequences of the invention contained in the Sequence Listing is also encompassed by the invention.  
     [0193] In preferred embodiments: the  H. pylori  polypeptide is encoded by a nucleic acid of the invention contained in the Sequence Listing, or by a nucleic acid having at least 60%, 70%, 80%, 90%, 95%, 98%, or 99% homology with a nucleic acid of the invention contained in the Sequence Listing.  
     [0194] In a preferred embodiment, the subject  H. pylori  polypeptide differs in amino acid sequence at 1, 2, 3, 5, 10 or more residues from a sequence of the invention contained in the Sequence Listing. The differences, however, are such that the  H. pylori  polypeptide exhibits an  H. pylori  biological activity, e.g., the  H. pylori  polypeptide retains a biological activity of a naturally occurring  H. pylori  polypeptide.  
     [0195] In preferred embodiments, the polypeptide includes all or a fragment of an amino acid sequence of the invention contained in the Sequence Listing; fused, in reading frame, to additional amino acid residues, preferably to residues encoded by genomic DNA 5′ or 3′ to the genomic DNA which encodes a sequence of the invention contained in the Sequence Listing.  
     [0196] In yet other preferred embodiments, the  H. pylori  polypeptide is a recombinant fusion protein having a first  H. pylori  polypeptide portion and a second polypeptide portion, e.g., a second polypeptide portion having an amino acid sequence unrelated to  H. pylori . The second polypeptide portion can be, e.g., any of glutathione-S-transferase, a DNA binding domain, or a polymerase activating domain. In preferred embodiment the fusion protein can be used in a two-hybrid assay.  
     [0197] Polypeptides of the invention include those which arise as a result of alternative transcription events, alternative RNA splicing events, and alternative translational and postranslational events.  
     [0198] The invention also encompasses an immunogenic component which includes at least one  H. pylori  polypeptide in an immunogenic preparation; the immunogenic component being capable of eliciting an immune response specific for the  H. pylori  polypeptide, e.g., a humoral response, an antibody response, or a cellular response. In preferred embodiments, the immunogenic component comprises at least one antigenic determinant from a polypeptide of the invention contained in the Sequence Listing.  
     [0199] In another aspect, the invention provides a substantially pure nucleic acid having a nucleotide sequence which encodes an  H. pylori  polypeptide. In preferred embodiments: the encoded polypeptide has biological activity; the encoded polypeptide has an amino acid sequence at least 60%, 70%, 80%, 90%, 95%, 98%, or 99% homologous to an amino acid sequence of the invention contained in the Sequence Listing; the encoded polypeptide has an amino acid sequence essentially the same as an amino acid sequence of the invention contained in the Sequence Listing; the encoded polypeptide is at least 5, 10, 20, 50, 100, or 150 amino acids in length; the encoded polypeptide comprises at least 5, preferably at least 10, more preferably at least 20, more preferably at least 50, 100, or 150 contiguous amino acids of the invention contained in the Sequence Listing.  
     [0200] In preferred embodiments: the nucleic acid of the invention is that contained in the Sequence Listing; the nucleic acid is at least 60%, 70%, 80%, 90%, 96%, 98%, or 99% homologous with a nucleic acid sequence of the invention contained in the Sequence Listing.  
     [0201] In a preferred embodiment, the encoded  H. pylori  polypeptide differs (e.g., by amino acid substitution, addition or deletion of at least one amino acid residue) in amino acid sequence at 1, 2, 3, 5, 10 or more residues, from a sequence of the invention contained in the Sequence Listing. The differences, however, are such that: the  H. pylori  encoded polypeptide exhibits a  H. pylori  biological activity, e.g., the encoded  H. pylori  enzyme retains a biological activity of a naturally occurring  H. pylori.    
     [0202] In preferred embodiments, the encoded polypeptide includes all or a fragment of an amino acid sequence of the invention contained in the Sequence Listing; fused, in reading frame, to additional amino acid residues, preferably to residues encoded by genomic DNA 5′ or 3′ to the genomic DNA which encodes a sequence of the invention contained in the Sequence Listing.  
     [0203] In preferred embodiments, the subject  H. pylori  nucleic acid will include a transcriptional regulatory sequence, e.g. at least one of a transcriptional promoter or transcriptional enhancer sequence, operably linked to the  H. pylori  gene sequence, e.g., to render the  H. pylori  gene sequence suitable for expression in a recombinant host cell.  
     [0204] In yet a further preferred embodiment, the nucleic acid which encodes an  H. pylori  polypeptide of the invention, hybridizes under stringent conditions to a nucleic acid probe corresponding to at least 8 consecutive nucleotides of the invention contained in the Sequence Listing; more preferably to at least 12 consecutive nucleotides of the invention contained in the Sequence Listing; more preferably to at least 20 consecutive nucleotides of the invention contained in the Sequence Listing; more preferably to at least 40 consecutive nucleotides of the invention contained in the Sequence Listing.  
     [0205] In a preferred embodiment, the nucleic acid encodes a peptide which differs by at least one amino acid residue from the sequences of the invention contained in the Sequence Listing.  
     [0206] In a preferred embodiment, the nucleic acid differs by at least one nucleotide from a nucleotide sequence of the invention contained in the Sequence Listing which encodes amino acids of the invention contained in the Sequence Listing.  
     [0207] In another aspect, the invention encompasses: a vector including a nucleic acid which encodes an  H. pylori  polypeptide or an  H. pylori  polypeptide variant as described herein; a host cell transfected with the vector; and a method of producing a recombinant  H. pylori  polypeptide or  H. pylori  polypeptide variant; including culturing the cell, e.g., in a cell culture medium, and isolating the  H. pylori  or  H. pylori  polypeptide variant, e.g., from the cell or from the cell culture medium.  
     [0208] In another aspect, the invention features, a purified recombinant nucleic acid having at least 50%, 60%, 70%, 80%, 90%, 95%, 98%, or 99% homology with a sequence of the invention contained in the Sequence Listing.  
     [0209] The invention also provides a probe or primer which includes a substantially purified oligonucleotide. The oligonucleotide includes a region of nucleotide sequence which hybridizes under stringent conditions to at least 8 consecutive nucleotides of sense or antisense sequence of the invention contained in the Sequence Listing, or naturally occurring mutants thereof. In preferred embodiments, the probe or primer further includes a label group attached thereto. The label group can be, e.g., a radioisotope, a fluorescent compound, an enzyme, and/or an enzyme co-factor. Preferably the oligonucleotide is at least 8 and less than 10, 20, 30, 50, 100, or 150 nucleotides in length.  
     [0210] The invention also provides an isolated  H. pylori  polypeptide which is encoded by a nucleic acid which hybridizes under stringent hybridization conditions to a nucleic acid contained in the Sequence Listing.  
     [0211] The invention further provides nucleic acids, e.g., RNA or DNA, encoding a polypeptide of the invention. This includes double stranded nucleic acids as well as coding and antisense single strands.  
     [0212] The  H. pylori  strain, from which genomic sequences have been sequenced, has been deposited in the American Type Culture Collection (ATCC # 55679; deposited by Genome Therapeutics Corporation, 100 Beaver Street, Waltham, Mass. 02154) as strain HP-J99.  
     [0213] Included in the invention are: allelic variations; natural mutants; induced mutants; proteins encoded by DNA that hybridizes under high or low stringency conditions to a nucleic acid which encodes a polypeptide of the invention contained in the Sequence Listing (for definitions of high and low stringency see Current Protocols in Molecular Biology, John Wiley &amp; Sons, New York, 1989, 6.3.1-6.3.6 and 6.4.1-6.4.10, hereby incorporated by reference); and, polypeptides specifically bound by antisera to  H. pylori  polypeptides, especially by antisera to an active site or binding domain of  H. pylori  polypeptide. The invention also includes fragments, preferably biologically active fragments. These and other polypeptides are also referred to herein as  H. pylori  polypeptide analogs or variants.  
     [0214] Putative functions have been determined for several of the  H. pylori  polypeptides of the invention, as shown in Table 1.  
     [0215] Accordingly, uses of the claimed  H. pylori  polypeptides based on these identified functions, as well as other functions as described herein, are also within the scope of the invention.  
     [0216] In addition, the present invention encompasses  H. pylori  polypeptides characterized as shown in Table 1 below, including:  H. pylori  cell envelope proteins,  H. pylori  secreted proteins,  H. pylori  cytoplasmic proteins and  H. pylori  cellular proteins. Members of these groups were identified by BLAST homology searches and by searches for secretion signal or transmembrane protein motifs. Polypeptides related by significant homology to the polypeptides of Table 1 are also considered to be classified in the manner of the homologs shown in Table 1.  
                       TABLE 1                       Previously-Filed ORF   ntSeqID   aaSeqID                                            A. CELL ENVELOPE PROTEINS               A.1 Flagella-associated proteins       hp6e12267_29298130_c2_52   1   4763       29298130   2   4764       12ge20305orf11   3   4765       01ce11016orf18   4   4766       hp6p80503_34199050_f1_3   5   4767       hp6p80503_3986693_f2_10   6   4768       01ce11016orf21   7   4769       hp6p80503_58562_f2_9   8   4770       hpy01ce11016orf_0013.aa   9   4771       01ce61016_58562_c1_111   10   4772       16219090   11   4773       05ep20322orf11   12   4774       hpy05ep20322orf_0014.aa   13   4775       hp6p12311_4428127_f2_2   14   4776       02ge10116_26382032_c3_129   15   4777       29454837   16   4778       02ge10116_29454837_c2_27   17   4779       02ge20116orf34   18   4780       02ge10116_24322632_c3_128   19   4781       02ge10116_32679687_c1_73   20   4782       04gp11701_16984442_f3_17   21   4783       16984442   22   4784       hp3e11168orf2   23   4785       22692187   24   4786       hp5p15212orf1   25   4787       02ce10213orf7   26   4788       hpy02ce10213orf_0001.aa   27   4789       hp5p15212_7289075_f2_5   28   4790       05ae11611orf1   29   4791       hpy05ae11611orf_0001.aa   30   4792       06ap30322_1986458_c3_31   31   4793       06ap10322_22663402_c2_31   32   4794       25525277   33   4795       06ep11202_25525277_c1_21   34   4796       06cp20302orf12   35   4797       203192   36   4798       06ep11202_6914712_c3_31   37   4799       06cp20302orf10   38   4800       1171928   39   4801       04cp11202_21699087_f1_9   40   4802       04ge11713orf5   41   4803       hpy13ap11517orf_0014.aa   42   4804       06gp71906_24417558_c2_160   43   4805       3942217   44   4806       06gp71906_3942217_c2_161   45   4807       hp3e11122orf5   46   4808       hpy11ap10314orf_0002.aa   47   4809       11ap10314orf2   48   4810       hpy11ap10314orf_0001.aa   49   4811       hp3e11122orf1   50   4812       06gp71906_4822162_c3_196   51   4813       14cp20718_24882763_f2_3   52   4814       24882763   53   4815       29zp10241orf6   54   4816       02cp20821orf3   55   4817       hp5p15861_4556533_f2_6   56   4818       01ap11502orf1   57   4819       14ee41924_4022217_f3_49   58   4820       917152   59   4821       07ap11213_23470127_f3_16   60   4822       hp2e10911orf5   61   4823       hp4e13394_3368767_c1_80   62   4824       09ap11406orf2   63   4825       hpy01ge10203orf_0004.aa   64   4826       hpy01ge10203orf_0002.aa   65   4827       hpy01ge10203orf_0001.aa   66   4828       01ge10203orf2   67   4829       hp1p13939_24322162_f3_17   68   4830       03ae10516orf14   69   4831       14gp12015orf6   70   4832       14gp12015orf8   71   4833       13727311   72   4834       06ep30223_13727311_c3_159   73   4835       hpy02ae11612orf_0023.aa   74   4836       01ce21104_4961067_c1_61   75   4837       01gp10401orf1   76   4838       26588588   77   4839       hp6p10590_42501_c2_32   78   4840       01gp10401orf5   79   4841       01gp10401orf4   80   4842       hpy01gp10401orf_0001.aa   81   4843       hp6p10590_4567317_c1_26   82   4844       12ap11614_24415965_f3_5   83   4845       12ap11614orf5   84   4846       19557055   85   4847       hp7e10429_24423462_c3_47   86   4848       07ge20415orf27   87   4849       07ge20415orf28   88   4850       26380318   89   4851       07ge20415orf34   90   4852       hp7e10429_4881342_c1_31   91   4853       07ge31107orf5   92   4854       07ge31107orf1   93   4855       hpy07ge31107orf_0001.aa   94   4856       07ge31107orf3   95   4857       07ge31107orf2   96   4858       hp6e50324_32692588_c2_11   97   4859       hpy02ae31112orf_0001.aa   98   4860       11ap20714_2165628_c2_83   99   4861       14ee11217orf3   100   4862       hpy14ee11217orf_0001.aa   101   4863       11ap20714_5271967_c1_60   102   4864       hp6p80503_13673962_f1_2   103   4865       21699087   9525   9637       25478375   9526   9638       36111066   9527   9639       A.2 Outer membrane       A.2.1 Terminal phe residue       09cp61003_14562637_c2_93   104   4866       12ge10305orf13   105   4867       03gp20123orf3   106   4868       12ge10305orf9   107   4869       hp6e12267_12931513_f1_11   108   4870       01ce61016_12931513_c2_106   109   4871       11ee10423orf2   110   4872       11ee10423orf1   111   4873       09cp61003_24063587_c1_74   112   4874       21503772   113   4875       30478562   114   4876       12ge10305orf10   115   4877       12ge10305orf1   116   4878       09cp61003_30478562_c3_106   117   4879       hp6e12267_30478562_f3_33   118   4880       1431462   119   4881       09cp11003_5860877_f3_7   120   4882       09cp61003_5860877_f2_23   121   4883       14ge10705orf5   122   4884       01cp11710orf16   123   4885       01cp11710orf5   124   4886       hpy01cp11710orf_0001.aa   125   4887       01cp11710orf6   126   4888       07ep11916_5913592_f3_18   127   4889       02ge10116_16803513_f2_34   128   4890       02gp20706_16803513_f1_1   129   4891       01cp11710orf18   130   4892       01cp11710orf9   131   4893       02gp20706_20365905_f2_8   132   4894       hpyhp5e15276orf_0007.aa   133   4895       02ge10116_23462_f2_43   134   4896       02gp20706_23632775_f3_32   135   4897       06ge10115orf4   136   4898       02ge10116_36367936_c1_19   137   4899       02ge10116_36367936_c1_92   138   4900       02ge20116orf25   139   4901       02ge10116_804550_f2_44   140   4902       hpyhp5e15276orf_0005.aa   141   4903       02ge41622_14875000_c2_65   142   4904       hpy13ee10216orf_0031.aa   143   4905       02ge11622_875260_f3_36   144   4906       13ee10216orf7   145   4907       12ge10610orf2   146   4908       hpy12ge10610orf_0002.aa   147   4909       05ep10815_26570332_c2_99   148   4910       06ee10207orf1   149   4911       hpy06ee10207orf_0001.aa   150   4912       hpy36zp10248orf_0001.aa   151   4913       14572133   152   4914       05ep10815_16131925_c2_97   153   4915       06ee10207orf2   154   4916       14ae21813orf2   155   4917       05ep10815_4719175_c1_83   156   4918       05ep10815_4719175_c1_115   157   4919       4960952   158   4920       04gp11701_4960952_c3_33   159   4921       hp3e11168orf30   160   4922       01cp20708_214843_c2_49   161   4923       hpyhp3e11168orf_0008.aa   162   4924       01cp20708_4960952_c1_43   163   4925       35156938   164   4926       hp5e15211_11676_f1_5   165   4927       hp5e15211orf15   166   4928       hp4e13462orf1   167   4929       14ep11115orf3   168   4930       hpy14ep11115orf_0001.aa   169   4931       11ee11408_4977193_c1_41   170   4932       05ae30220_4977193_c3_198   171   4933       917200   172   4934       05ae30220_917200_c3_172   173   4935       07ap80601orf5   174   4936       07ap80601_917200_f3_10   175   4937       05ae30220_9882767_f2_34   176   4938       05ae20220orf37   177   4939       06ae11016_4729625_c3_68   178   4940       hpy07ee20513orf_0008.aa   179   4941       24132293   180   4942       07ee20513orf28   181   4943       09ae10512_48768_c3_67   182   4944       06ep10306orf1   183   4945       hpy06ep10306orf_0001.aa   184   4946       01ae12001_24218781_f2_18   185   4947       07gp11807orf17   186   4948       hpy07gp11807orf_0026.aa   187   4949       11ae11922_12586675_f2_1   188   4950       14ap10221_13689381_c3_4   189   4951       hp6p10903_4398263_f3_6   190   4952       05gp11901orf19   191   4953       06ap10609_12586675_f2_19   192   4954       06ap11119_24426508_f3_26   193   4955       06ap11119_24426508_f3_27   194   4956       07ae10923_24426508_f1_1   195   4957       11ge10309orf28   196   4958       4740887   197   4959       hp1p11256orf7   198   4960       05ee10816_14495437_f2_13   199   4961       06ep10615_14495437_f3_47   200   4962       05ee10816_4103408_f2_11   201   4963       04ce11617orf16   202   4964       hpy06ap10305orf_0002.aa   203   4965       hpy06ap10305orf_0001.aa   204   4966       06ep10615_49068_c2_87   205   4967       23646885   206   4968       04cp11202_23646885_f2_26   207   4969       01ae12021orf8   208   4970       06gp71906_35158328_f3_85   209   4971       hpy02cp11822orf_0009.aa   210   4972       hpy12gp11106orf_0001.aa   211   4973       06gp71906_3941642_f2_70   212   4974       02ap21113orf2   213   4975       06gp71906_970325_c3_190   214   4976       14ap10815_20585777_c1_13   215   4977       hp3e10349orf27   216   4978       07ap11015orf2   217   4979       hp5p15580orf1   218   4980       07ep30818orf4   219   4981       hpy07ep30818orf_0002.aa   220   4982       07ap11015orf4   221   4983       07ap11015_23938312_f3_2   222   4984       02ep30607orf19   223   4985       07ee11402_2458267_c3_108   224   4986       14ee41924_2458267_c2_93   225   4987       hp5p15641_12195281_c1_24   226   4988       hp5p15612orf2   227   4989       hp5p15641_21698387_c2_20   228   4990       hp5p15641orf23   229   4991       02ae31010_30208317_f1_14   230   4992       hp1p13947orf10   231   4993       06cp11217_4881263_f2_9   232   4994       hp1p13852orf7   233   4995       hpy06cp10117orf_0002.aa   234   4996       06cp10117orf2   235   4997       05ce10420orf1   236   4998       09cp10224_1062966_c3_61   237   4999       09cp10224_1062966_c1_44   238   5000       09cp10224_1412715_c3_56   239   5001       01ce10516orf20   240   5002       09cp10224_1962590_f3_31   241   5003       1962590   242   5004       01ce10516orf2   243   5005       hpy03ge10505orf_0004.aa   244   5006       03ge10505orf2   245   5007       hpy03ge10505orf_0002.aa   246   5008       hpyhp3e10302orf_0003.aa   247   5009       hp3e10302orf17   248   5010       06cp30603_679218_f2_34   249   5011       hpy05gp11608orf_0002.aa   250   5012       hpy02ee10520orf_0001.aa   251   5013       hpy05gp11608orf_0001.aa   252   5014       13ae10610_6522827_c3_37   253   5015       13ae10610_156411_c3_33   254   5016       04ge10816_22086531_f2_10   255   5017       04ge10816_859692_f3_12   256   5018       04ge11210orf1   257   5019       04ge11210orf2   258   5020       hpy04ge11210orf_0001.aa   259   5021       13ae10610_859692_c2_32   260   5022       13ap10511_12891718_c1_2   261   5023       hpy13ap10511orf_0001.aa   262   5024       32609403   263   5025       11ee11408_10584582_c3_51   264   5026       05gp11901orf25   265   5027       03ae10804_12609533_c1_26   266   5028       06ep10306orf10   267   5029       hpyhp5e11726orf_0008.aa   268   5030       06ep11509_35954752_f2_1   269   5031       hp2p10625orf7   270   5032       03ae10804_14495437_c2_38   271   5033       03ee11215orf39   272   5034       hp4e53394_11798952_c2_101   273   5035       06ep30223_34409437_f3_94   274   5036       hp3e11024orf5   275   5037       hp3e11024orf23   276   5038       06ep30223_34409437_f1_34   277   5039       06ep30223_34409437_f2_64   278   5040       29ge10111orf1   279   5041       29ge10111orf3   280   5042       06ce20610_1367157_f1_8   281   5043       14ee10308orf1   282   5044       14ee10308orf7   283   5045       02gp20814_3984818_f1_1   284   5046       hpy14ee10308orf_0009.aa   285   5047       11ae12004_4298568_c3_51   286   5048       hpy14ee10308orf_0008.aa   287   5049       11ae12004_3367666_c2_41   288   5050       06ge20501_4298568_c3_53   289   5051       10553192   290   5052       11ae80818_10553192_f2_16   291   5053       06cp11722orf16   292   5054       11ae80818_19632781_c3_57   293   5055       06cp11722orf11   294   5056       14ce61516_24609816_f2_9   295   5057       03ap21820orf8   296   5058       11ae80818_7952_c1_49   297   5059       hpy07ee11519orf_0002aa   298   5060       hpy02ce10114orf_0004.aa   299   5061       07ee11519orf1   300   5062       hpy07ee11519orf_0001.aa   301   5063       hp7e10192_5869188_c3_17   302   5064       05ce10910_25598277_f3_3   303   5065       hp7e10192_25598277_c2_15   304   5066       11ap20714_2077_c3_103   305   5067       hpyhp5e15440orf_0009.aa   306   5068       04gp11803orf11   307   5069       04gp11803orf13   308   5070       06ap20306_23437632_f3_9   309   5071       11ap20714_34023312_f3_46   310   5072       hp3e10057orf3   311   5073       14ee11217orf4   312   5074       14ee11217orf2   313   5075       11ap20714_4960432_c3_97   314   5076       hpy04gp11803orf_0013.aa   315   5077       05ap21216orf4   316   5078       07ap20216_7227202_f3_10   317   5079       11ap20714_7227202_f3_40   318   5080       11ap20714_7227202_f3_43   319   5081       hp1p14013_11726503_c2_20   320   5082       hp1p14013orf17   321   5083       02cp10615_21908138_f1_4   322   5084       hpyhp1p14013orf_0008.aa   323   5085       hp3p10304orf2   324   5086       02cp10615_26573462_c1_45   325   5087       31250333   9528   9640       24488537   9529   9641       16225006   9530   9642       486075   9531   9643       2458267   9532   9644       26614041   9533   9645       23567137   9535   9534       1367157   9535   9647       A.2.2 Terminal phe residue and C-       terminal tyrosine cluster       06ee10709_21675012_f1_2   326   5088       06ee10709orf2   327   5089       hpy13ee10216orf_0036.aa   328   5090       02ge41622_34176513_c1_50   329   5091       01cp20708_36134808_f2_11   330   5092       14ee10419orf5   331   5093       hpy14ee10419orf_0001.aa   332   5094       01ce10320_30273587_f3_38   333   5095       07ge11521_14160930_f3_23   342   5104       hpy29ap11902orf_0001.aa   343   5105       29ap11902orf1   344   5106       29ap10306orf3   345   5107       hpy29ap10306orf_0003.aa   346   5108       04ep41903_26757937_f1_2   347   5109       04ep41903_26757937_f3_16   348   5110       14ee21118orf2   349   5111       hpy04cp11809orf_0001.aa   350   5112       hp3p11121_14454686_f2_1   351   5113       14ee21118orf1   352   5114       hpy04cp11809orf_0002.aa   353   5115       04ep41903_4101593_f2_10   354   5116       116018   355   5117       01ae12001_116018_c2_40   356   5118       06ap10609_116018_c3_50   357   5119       07gp11807orf48   358   5120       05ee10816_36126938_f3_16   359   5121       36126938   360   5122       06ep10615_36126938_f1_14   361   5123       04ce11617orf2   362   5124       01ge10801orf2   369   5131       hpy01ge10801orf_0001.aa   370   5132       hpyhp3p11022orf_0002.aa   371   5133       hpyhp3p11022orf_0001.aa   372   5134       02ae31010_12504512_f3_28   373   5135       hpy12ap11614orf_0001.aa   374   5136       hpyhp5p15641orf_0002.aa   375   5137       hp7e10520_14728137_f1_1   376   5138       02ae31010_417818_f3_29   377   5139       01ge10801orf3   378   5140       3964593   379   5141       06cp30603_4687507_f1_7   380   5142       06cp30603_4687507_f1_9   381   5143       05cp20518orf9   382   5144       hpy04ge11210orf_0003.aa   383   5145       13ae10610_26855313_f3_15   384   5146       13ae10610orf2   385   5147       hpy13ae10610orf_0001.aa   386   5148       13ae10610orf1   387   5149       13ae10610_33726080_f1_1   388   5150       13ae10610_35912_f2_3   389   5151       hp3p11086orf2   390   5152       hp3p11086orf1   391   5153       hp5p15575_33445317_f2_20   392   5154       hpy06cp11722orf_0001.aa   393   5155       06cp11722orf5   394   5156       11ae80818_7290627_c2_51   395   5157       hp7e10590_26172564_c1_68   396   5158       hp7e10590_7072317_c1_70   397   5159       4687507   9536   9648       A.2.3 C-terminal tyrosine cluster motif       hpy02ap10310orf_0004.aa   334   5096       02ap10310orf2   335   5097       02ap10310orf1   336   5098       02ap10310_30089800_f3_3   337   5099       02ap10310orf3   338   5100       hpy02ap10310orf_0001.aa   339   5101       02ap10310_22789651_f1_1   340   5102       04ep41903_21564052_c2_56   341   5103       hpy29ep20112orf_0001.aa   363   5125       29ep20112orf2   364   5126       06gp71906_20486556_f2_65   365   5127       06ep10615_961562_f2_41   366   5128       06ep10615_961562_f1_15   367   5129       06gp71906_961562_f3_117   368   5130       06gp10108orf2   398   5160       hpy06gp10108orf_0003.aa   399   5161       09cp61003_492187_c2_80   400   5162       01ce61016_492187_c3_120   401   5163       01ce10516orf21   402   5164       09cp10224_429510_c2_46   403   5165       07cp21714orf2   404   5166       07cp21714orf1   405   5167       07cp21714orf3   406   5168       07ce11019_22051291_f1_1   407   5169       14gp11423_26803801_f1_1   408   5170       06cp11217_19720300_f3_11   409   5171       hp4e53394_22864682_c2_86   410   5172       hp4e53394_19720300_c3_98   411   5173       hp1p13852orf6   412   5174       hp4e53394_26209843_c3_98   413   5175       hp4e53394_26756900_c3_103   414   5176       hp6p12244_5273452_c2_82   415   5177       01cp11414orf2   416   5178       07gp11909_26460892_f2_6   424   5186       02ap11117_26460892_f1_15   425   5187       A.2.4 Via homology       hp6e12267_4721061_c1_41   417   5179       09cp61003_4721061_f1_16   418   5180       4721061   419   5181       12ge20305orf2   420   5182       09cp11003_5945252_f2_4   421   5183       09cp61003_5945252_f1_5   422   5184       14ge10705orf3   423   5185       01ae11010_40688_c2_38   426   5188       hp4p33322_40688_c1_38   427   5189       hp3e11075orf3   428   5190       14ce31519orf1   429   5191       14ce11519orf4   430   5192       04ep41903_4538588_f3_21   431   5193       04ep41903_4538588_f2_20   432   5194       05ae11108orf1   433   5195       hpy05ae11108orf_0001.aa   434   5196       03ce10801orf1   435   5197       hpy03ce10801orf_0001.aa   436   5198       03ce10801orf4   437   5199       05ae30220_4487562_c1_101   438   5200       05ae30220_26834500_c2_164   439   5201       5083193   440   5202       05ae30220_5083193_c3_165   441   5203       07ap80601orf8   442   5204       07ap80601_5083193_f3_8   443   5205       hp5p15212_13729635_c3_35   444   5206       06gp10409_3398427_f2_12   445   5207       13ae10511orf3   446   5208       hpy13ae10511orf_0001.aa   447   5209       06gp10409_3398427_f2_12   448   5210       06gp71906_2003143_c3_217   449   5211       hpy16cp30109orf_0001.aa   450   5212       4490717   451   5213       hpy16cp30109orf_0002.aa   452   5214       16cp30109orf6   453   5215       04cp11202_4490717_f2_29   454   5216       hpy06ce31218orf_0001.aa   455   5217       14ee41924_1046877_c3_104   456   5218       07ee11402_1046877_c3_100   457   5219       hpy02ep30607orf_0014.aa   458   5220       hpy02ep30607orf_0012.aa   459   5221       02ep30607orf27   460   5222       14ee41924_23527267_c3_107   461   5223       07ee11402_10759567_c2_86   462   5224       hpy02ep30607orf_0015.aa   463   5225       14ee41924_33203917_c2_85   464   5226       3906963   465   5227       04ee11108_3906963_f1_7   466   5228       27ze10351orf5   467   5229       06cp30603_23476568_c1_44   468   5230       978477   469   5231       05cp20518orf39   470   5232       06cp30603_978517_c3_137   471   5233       hpy04gp11213orf_0009.aa   472   5234       hpy02ce12007orf_0003.aa   473   5235       hpy02ce12007orf_0002.aa   474   5236       hp1p13939orf13   475   5237       05ce10208_4766691_c2_18   476   5238       4766691   477   5239       hpyhp1p13939orf_0001.aa   478   5240       hp1p13939_21641016_f1_1   479   5241       hp4p62853_4766691_f3_23   480   5242       03xe11215orf7   481   5243       03xe11215orf8   482   5244       02ae11612_22477267_f2_27   483   5245       02gp20814_24415958_f3_9   484   5246       14ee10308orf4   485   5247       09cp21607_31262_c2_11   486   5248       31262   487   5249       07gp31516orf4   488   5250       35887   489   5251       11ap20714_4797137_f3_45   490   5252       hp3e10057orf2   491   5253       A.2.5 Other outer membrane proteins       04ep41903_23867687_c2_32   492   5254       23867687   493   5255       09ae11601orf11   494   5256       06ep10615_9842_f3_46   495   5257       06ep10615_189160_f3_47   496   5258       hpy05ep11717orf_0008.aa   497   5259       05ep11717orf2   498   5260       06ep10615_9842_f1_5   499   5261       hpy02ae20216orf_0001.aa   500   5262       hpy02ce30710orf_0006.aa   501   5263       02ae20216orf2   502   5264       hpyhp1e10506orf_0008.aa   503   5265       05cp21223_29308518_c2_24   504   5266       06ep30223_104052_c2_138   505   5267       05cp21223_23831400_c1_22   506   5268       hp1e10506orf22   507   5269       14cp10923_4414000_f3_11   508   5270       4414000   509   5271       14cp10923orf8   510   5272       A.3 Inner membrane       A.3.1 Proteins involved in transport       hp6e12267_1069213_f3_31   511   5273       12ge20305orf35   512   5274       11132778   513   5275       hp6e12267_11132778_c2_48   514   5276       12ge10305orf16   515   5277       20032561   516   5278       09ap20802orf27   517   5279       hp2p10272_4100312_c2_35   518   5280       02ge10116_24238762_c1_22   519   5281       24238762   520   5282       02ge20116orf28   521   5283       hpyhp3e11168orf_0011.aa   522   5284       23492181   523   5285       23853165   524   5286       hp3e11168orf29   525   5287       04gp11701_23442187_c3_32   526   5288       14ce31519_15635927_f3_15   527   5289       02ce10809orf6   528   5290       02ce10809orf16   529   5291       14ce31519_24650009_c1_17   530   5292       04ep41903_16667055_c1_37   531   5293       04ep41903_19689182_c1_43   532   5294       2461062   533   5295       1179838   534   5296       05ae30220_4954526_f3_53   535   5297       05ae20220orf32   536   5298       05ae20220orf54   537   5299       05ae30220_1179838_f2_53   538   5300       5878208   539   5301       27ze10351orf29   540   5302       23728388   541   5303       27ze10351orf24   542   5304       04ee11108_23728388_c2_22   543   5305       35345228   544   5306       04ee11108_3914683_c3_27   545   5307       27ze10351orf18   546   5308       hpy27ze10351orf_0001.aa   547   5309       05ae30220_3914683_c1_106   548   5310       11924177   549   5311       04ee11108_56313_f1_9   550   5312       27ze10351orf7   551   5313       01ae12001_14714687_f2_16   552   5314       14714687   553   5315       07gp11807orf9   554   5316       07gp11807orf29   555   5317       19531291   556   5318       42683   557   5319       07gp11807orf49   558   5320       01ae12001_19536375_c2_41   559   5321       01ae12001_3319687_f1_10   560   5322       3319687   561   5323       07gp11807orf25   562   5324       5875152   563   5325       01ae12001_5875152_f2_15   564   5326       07gp11807orf8   565   5327       06ap11119_9860256_f1_4   566   5328       hp6p10723orf11   567   5329       01ce11513orf29   568   5330       01ce11513orf33   569   5331       1464715   570   5332       06cp11118_1464715_c2_19   571   5333       06cp11118_26823300_c1_15   572   5334       06cp11118_213561_c1_15   573   5335       01ce11513orf22   574   5336       12ap11619orf1   575   5337       hpy12ap11619orf_0001.aa   576   5338       06cp11118_391525_c2_18   577   5339       06ep10615_15659509_c1_51   578   5340       06ep10615_5203401_c3_87   579   5341       11cp10113orf4   580   5342       hpy11cp10113orf_0001.aa   581   5343       29zp10241orf4   582   5344       14cp20718_20210817_f1_1   583   5345       07ap61111_33330468_f1_6   584   5346       12ae11420orf1   585   5347       hpy12ae11420orf_0001.aa   586   5348       14ee41924_24001568_f2_40   587   5349       07ee11402_24001568_f1_15   588   5350       07ae11611orf1   589   5351       hpy07ae11611orf_0001.aa   590   5352       07ee11402_32431453_f1_1   591   5353       24609593   592   5354       14ce11113orf1   593   5355       hpy14ce11113orf_0001.aa   594   5356       07ee11402_5127343_f3_2   595   5357       hpy14ae20424orf_0002.aa   596   5358       04ae61517_1048812_f1_1   597   5359       04ae21517orf1   598   5360       04ae61517_24802342_f2_3   599   5361       07ee50709_411457_f2_68   600   5362       10723412   601   5363       02ae31010_4455467_c2_84   602   5364       02ce11022orf8   603   5365       07ae32002orf3   604   5366       hpy07ae32002orf_0002.aa   605   5367       hp4p13446orf10   606   5368       hp6p10904_22069680_f3_12   607   5369       hpy06gp11202orf_0001.aa   608   5370       hpy06gp111202orf_0002.aa   609   5371       33399142   610   5372       06gp11202orf7   611   5373       hpy06gp11202orf_0003.aa   612   5374       09ce10413_24428452_f1_5   613   5375       hp6p10904_6726062_f3_13   614   5376       09ce10413_26734687_f3_23   615   5377       hpyhp3e10302orf_0001.aa   616   5378       29479681   617   5379       05cp20518orf33   618   5380       06cp30603_22666625_f3_58   619   5381       06cp30603_664083_c1_94   620   5382       06cp30603orf10   621   5383       06cp30603orf9   622   5384       06cp30603_36359687_c3_78   623   5385       06cp30603_36359687_c3_161   624   5386       09cp10713_36359687_c1_119   625   5387       16406265   626   5388       12ge10321_4691563_f2_6   627   5389       hp4p11352orf4   628   5390       12ae10622_24406450_f1_9   629   5391       179677   630   5392       03ae10804_22459680_f1_4   631   5393       hp5e11726orf7   632   5394       hp1e80523_3906693_f1_2   633   5395       hp3e10929orf1   634   5396       hp3e11188_26359712_f2_3   635   5397       06ep11108orf13   636   5398       hpy06ep11108orf_0009.aa   637   5399       01ep10216orf9   638   5400       hp4e13394_19656577_f3_57   639   5401       hp5e13045orf1   640   5402       02ae11611orf11   641   5403       hp$$0$$ae11611orf_0005.aa   642   5404       hp4e53394_24744002_f1_1   643   5405       hp5e13045_24744002_f1_1   644   5406       hp1p13939_817683_c3_36   645   5407       hp4p62853_15818754_c2_42   646   5408       783432   647   5409       05cp11911orf27   648   5410       02ae11612_14641535_c1_57   649   5411       02ae11612_4338438_c3_91   650   5412       4338438   651   5413       05cp11911orf41   652   5414       hp5p15575_292564_c2_38   653   5415       hp5e15084orf6   654   5416       hp6e10491_31757638_f1_1   655   5417       24416083   656   5418       13ae10712orf4   657   5419       13ae10712_24416083_f2_10   658   5420       05gp20111orf1   659   5421       06ce20610_4086003_c1_28   660   5422       13ae10712orf9   661   5423       22379952   662   5424       13ae10712_4725342_f1_4   663   5425       03ap21820orf5   664   5426       03ap21820orf17   665   5427       11ae80818_36131282_c3_64   666   5428       10353192   667   5429       12ap10324_16422591_f3_4   668   5430       12ap10324orf2   669   5431       01ce11513orf21   670   5432       02ep20506_4882763_c2_19   671   5433       hp7p10287_4882763_f2_9   672   5434       33203192   673   5435       06ap11418_33203192_f1_1   674   5436       hp5e15440orf16   675   5437       03ae11503_4532892_c2_19   676   5438       03ae11503orf10   677   5439       4826401   678   5440       01ae22001orf5   679   5441       01ae22001orf2   680   5442       01ae22001orf1   681   5443       02cp10615_33877086_c3_61   682   5444       22453166   9537   9649       875042   9538   9650       19536375   9539   9651       10675632   9540   9652       24218968   9541   9653       13726562   9542   9654       36131282   9543   9655       16422591   9544   9656       4882763   9545   9657       36573502   9546   9658       A.3.1.1 Proteins involved in amino acid       metabolism &amp; transport       2042312   683   5445       02gp20706_2042312_c1_44   684   5446       01cp11710orf34   685   5447       04ap20119orf5   686   5448       01cp10707orf2   687   5449       02ge11810_32538290_f1_4   688   5450       02ge11810_867187_f3_10   689   5451       02ge11810_867187_f3_9   690   5452       02ce10809orf2   691   5453       02ce10809orf4   692   5454       14ce31519_16042_f2_11   693   5455       02ce10809orf5   694   5456       14ce31519_9960937_f3_14   695   5457       24215   696   5458       06gp10409_80130_c2_24   697   5459       06gp10409_80130_c1_24   698   5460       04ep10811orf4   699   5461       02ae31522orf4   700   5462       02ae31522orf3   701   5463       14gp11720_3906693_c3_4   702   5464       02ae41522_3906693_c2_3   703   5465       35269000   704   5466       02ae11611orf1   705   5467       hpy02ae11611orf_0001.aa   706   5468       hp4e53394_30602256_c3_124   707   5469       hp5e13045_4454655_c3_14   708   5470       hp4e53394_4460905_c3_121   709   5471       hpy14ce10720orf_0001.aa   710   5472       01ee11622orf2   711   5473       hpy01ee11622orf_0001.aa   712   5474       hp5p15575_1955465_c3_41   713   5475       02cp11813orf1   714   5476       hp6e50324_24507825_c3_12   715   5477       03ae10804orf2   716   5478       hp5e11726orf2   717   5479       hp5e11726orf4   718   5480       36203402   719   5481       03ae10804_192208_f2_9   720   5482       5083577   9547   9659       289711   9548   9660       “A.3.1.2 Involved in nucleotide, lipid, or       cofactor metabolism &amp; transport”       02gp20706_24415918_f3_20   721   5483       01cp11710orf2   722   5484       12ae11404orf16   723   5485       12ae11404orf17   724   5486       12ae11404_19963577_c3_15   725   5487       12ae11404orf14   726   5488       12ae11404orf15   727   5489       12ae11404orf11   728   5490       hpy12ae11404orf_0011.aa   729   5491       05ee11015_26375799_c1_10   730   5492       12ae11404_992256_c3_14   731   5493       09ae10512orf3   732   5494       09ae10512orf1   733   5495       hpy09ae10512orf_0001.aa   734   5496       07ee20513orf11   735   5497       09ae10512_23942135_f1_1   736   5498       06ae11016_14063177_f3_23   737   5499       06gp71906_25478192_c1_131   738   5500       hp3e11122orf3   739   5501       29gp20307orf1   740   5502       hpy11ee21702orf_0001.aa   741   5503       11ee21702orf1   742   5504       hp5p15641_4335890_c2_27   743   5505       02ae31010_5085162_c1_47   744   5506       07ce10203orf11   745   5507       hpy05cp20518orf_0001.aa   746   5508       24824087   747   5509       hpy05cp20518orf_0002.aa   748   5510       06cp30603orf11   749   5511       06cp30603_14663917_c1_51   750   5512       06cp30603_24256503_c1_110   751   5513       01ge10214orf1   752   5514       hpy01ge10214orf_0001.aa   753   5515       hp3e11188_24417800_f2_4   754   5516       03ee11215_22367062_c2_29   755   5517       03ee11215orf38   756   5518       01ge10203orf1   757   5519       01ge10203orf3   758   5520       01ge10203_4875375_f2_3   759   5521       hp3e11024orf8   760   5522       hp3e11024orf18   761   5523       06ep30223_204582_f1_39   762   5524       02cp11721orf20   763   5525       hp7e10100_5350012_c2_18   764   5526       24806290   9549   9661       A.3.1.3 Proteins involved in inorganic       ion transport       01cp11710orf28   765   5527       01cp11710orf37   766   5528       02gp20706_24645837_c3_64   767   5529       02gp20706_4879625_f2_9   768   5530       02ge10116_4879625_f3_58   769   5531       01cp11710orf10   770   5532       06gp10409orf9   771   5533       04ep10811orf2   772   5534       06gp10409orf8   773   5535       06gp10409orf7   774   5536       hpy06gp10409orf_0004.aa   775   5537       06gp10409_21751016_c3_26   776   5538       06gp10409_4881318_c1_21   777   5539       06gp10409_4881318_c3_30   778   5540       14ce20219orf2   779   5541       14ce20219orf1   780   5542       14ce20219orf4   781   5543       06gp71906_667968_f3_66   782   5544       09cp10502orf18   783   5545       09cp10502orf11   784   5546       07ae11008_250652_c3_59   785   5547       07ae11008_15806538_c1_38   786   5548       16406581   787   5549       07ce11019_3931943_c1_11   788   5550       07cp21714orf13   789   5551       114505   790   5552       11ap11902orf3   791   5553       hpy11ap11902orf_0001.aa   792   5554       11ae80818_11188791_c3_60   793   5555       06cp11722orf12   794   5556       11ae80818_25593768_c1_44   795   5557       14cp11908_25593768_c3_97   796   5558       11ae80818_6828218_f2_19   797   5559       6828218   798   5560       06cp11722orf21   799   5561       24645837   9550   9662       3203142   9551   9663       34666680   9552   9664       22441050   9553   9665       26258562   9554   9666       A.3.1.4 Proteins involved in       carbohydrate metabolism &amp; transport       21742157   800   5562       07ce11409orf4   801   5563       14ee41924_5865675_f1_18   802   5564       A.3.2 Proteins involved in outer       membrane and cell wall formation       hp6p80503_235782_f3_18   803   5565       01ce11016orf19   804   5566       01ce11016orf22   805   5567       hp6p80503_26593953_f1_4   806   5568       01ce61016_26593953_c1_113   807   5569       14ce11503orf3   808   5570       09cp11003_24646926_c2_13   809   5571       14ge10705orf11   810   5572       hpy14ge10705orf_0005.aa   811   5573       09cp61003_29322967_c1_75   812   5574       04ap20119orf2   813   5575       02ge11810_35282625_f3_9   814   5576       06cp20302orf1   815   5577       hpy06cp20302orf_0001.aa   816   5578       06ep11202_24219093_c3_30   817   5579       hpy06ap10209orf_0005.aa   818   5580       06ap10209orf3   819   5581       25992137   820   5582       04ge11713orf37   821   5583       hpy04ge11713orf_0020.aa   822   5584       04cp11202_3178500_c1_76   823   5585       04cp11202_3178500_c3_110   824   5586       11ep12011orf5   825   5587       11ep12011orf9   826   5588       06gp71906_3945965_c2_153   827   5589       13ap11517orf25   828   5590       13ap11517orf20   829   5591       06cp10411orf6   830   5592       12gp31106_5267037_c1_43   831   5593       06gp71906_5267037_f3_106   832   5594       07ae11008_24409577_c3_56   833   5595       07ae11008_24409577_c1_37   834   5596       25605166   835   5597       04ee11108_954683_c1_15   836   5598       27ze10351orf17   837   5599       14gp12015orf16   838   5600       hp2e10229orf2   839   5601       14gp12015orf13   840   5602       06ep30223_4698838_f2_55   841   5603       03ap21820orf9   842   5604       03ap21820orf3   843   5605       03ap21820orf11   844   5606       11ae80818_24415917_c1_45   845   5607       hpy02cp11721orf_0004.aa   846   5608       02cp11721orf14   847   5609       hp7e10100_1359450_c1_14   848   5610       22460468   9555   9667       23468781   9556   9668       495312   9557   9669       5267037   9558   9670       4698838   9559   9671       24415917   9560   9672       A.3.3 Proteins involved in energy       conversion       hpy07ge20415orf_0003.aa   849   5611       hp7e10429_7132780_c2_38   850   5612       06ge10217_32615840_f3_1   851   5613       06ge10217orf1   852   5614       01ce11016orf8   853   5615       hp6p80503_6845042_c2_25   854   5616       01ce61016_24492212_f1_1   855   5617       01ce11016orf1   856   5618       hp6p80503_992202_c1_21   857   5619       hpy04ce11408orf_0002.aa   858   5620       hp2p10272_5211682_c3_44   859   5621       04ce11408orf2   860   5622       02ap11117orf1   861   5623       hp6p21623_6906328_c2_51   862   5624       09ge11703orf1   863   5625       14ce31519_29339030_c2_27   864   5626       09ge11703orf2   865   5627       hpy09ge11703orf_0001.aa   866   5628       hpyhp3p10366orf_0001.aa   867   5629       hp3p10366orf2   868   5630       14ce31519_3364062_c1_21   869   5631       06cp11118orf5   870   5632       06cp11118_24620968_c3_24   871   5633       hpy06cp11118orf_0002.aa   872   5634       06cp11118orf8   873   5635       06cp11118_25445342_c2_20   874   5636       hpy06cp11118orf_0006.aa   875   5637       16412593   876   5638       06cp11118orf6   877   5639       06cp11118_4532588_c3_23   878   5640       04ge11613orf5   879   5641       04ge11613orf4   880   5642       hp5e15419orf2   881   5643       06gp10409_24406465_c3_27   882   5644       3953143   883   5645       02ae31010_29308453_f2_25   884   5646       hp1p13947orf2   885   5647       01ce10516orf18   886   5648       hpy01ce10516orf_0011.aa   887   5649       01ce10516orf17   888   5650       09cp10224_23570302_c1_37   889   5651       09cp10224_32042937_c1_39   890   5652       01ce10516orf25   891   5653       hp1p11244orf5   892   5654       09cp10224_35585952_c1_41   893   5655       01ce10516orf24   894   5656       01ce10516orf19   895   5657       01ce10516orf15   896   5658       09cp10224_4484718_c1_38   897   5659       11cp71403orf5   898   5660       09ze10333_4962817_f3_12   899   5661       16ae10113orf1   900   5662       16ae10113orf3   901   5663       hpy07ee11620orf_0001.aa   902   5664       16ae10113orf2   903   5665       hpy16ae10113orf_0001.aa   904   5666       09ze10333_4962817_f3_11   905   5667       05ae20220orf93   906   5668       hp2e51852_23711552_c3_4   907   5669       16131887   908   5670       2082012   909   5671       09ap11406orf14   910   5672       09ap11406orf5   911   5673       hp4e13394_1353438_c2_103   912   5674       02ap71220orf3   913   5675       02ap71220orf2   914   5676       hp4e13394_5964452_c2_97   915   5677       hp4e13394_15828963_c2_90   916   5678       6093906   917   5679       hp4e13394_2042837_c3_122   918   5680       09ap11406orf15   919   5681       11ce11603orf14   920   5682       11ce11603orf23   921   5683       hp4e13394_23446016_c3_119   922   5684       hp4e13394_23915877_c1_75   923   5685       23915877   924   5686       11ce11603orf6   925   5687       hpy11ce11603orf_0004.aa   926   5688       11ce11603orf2   927   5689       11ce11603orf19   928   5690       hp4e13394_29977187_c1_72   929   5691       hpy09ap11406orf_0001.aa   930   5692       1204418   931   5693       11ce11603orf16   932   5694       hp4e13394_32218775_c2_102   933   5695       4035783   934   5696       hp4e13394_3908568_c3_120   935   5697       11ce11603orf25   936   5698       11ce11603orf13   937   5699       11ce11603orf12   938   5700       hpy11ce11603orf_0008.aa   939   5701       11ce11603orf3   940   5702       11ce11603orf22   941   5703       hp4e13394_490786_c1_73   942   5704       hp4e13394_677292_c3_121   943   5705       11ce11603orf26   944   5706       hp4e53394_29422028_c1_84   945   5707       hp1e10523orf1   946   5708       hp1e10523orf2   947   5709       hpyhp1e10523orf_0001.aa   948   5710       01ce11104_4589005_c3_14   949   5711       hp5p15575orf14   950   5712       05cp10201orf1   951   5713       hpy05cp10201orf_0001.aa   952   5714       hp5p15575orf16   953   5715       hpyhp5p15575orf_0007.aa   954   5716       13ep10801orf2   955   5717       hp5p15575_24246012_c2_39   956   5718       hp6p10590_30521093_f2_14   957   5719       11ge11422orf3   958   5720       07ce11206orf1   959   5721       hp6p10590_4817177_f3_22   960   5722       hpy05gp20111orf_0005.aa   961   5723       05gp20111orf12   962   5724       06ce20610_4331338_f3_18   963   5725       01gp11016_17070306_c2_16   964   5726       hpy01gp11016orf_0003.aa   965   5727       01gp11016orf20   966   5728       01gp11016orf16   967   5729       01gp11016_12583568_c1_10   968   5730       01gp11016_1442187_c3_18   969   5731       01gp11016orf19   970   5732       hp7e10434_1442187_f3_19   971   5733       hp7e10434_210827_f2_11   972   5734       hpy01gp11016orf_0006.aa   973   5735       12ap10324orf7   974   5736       12ap10324_23531562_f2_2   975   5737       12ap10324_16052038_f1_1   976   5738       12ap10324orf1   977   5739       hpy12ap10324orf_0001.aa   978   5740       hp7e30434_24651068_f3_32   979   5741       01gp11016orf11   980   5742       01gp11016orf12   981   5743       hpy01gp11016orf_0001.aa   982   5744       hp7e10434_24744012_f2_14   983   5745       hp7e30434_24744012_f3_31   984   5746       01gp11016orf13   985   5747       01gp11016_4103403_c2_13   986   5748       5869090   987   5749       01gp11016_5869090_c2_14   988   5750       01gp11016orf14   989   5751       06ap11418_31813750_c2_24   990   5752       hp5e15440orf8   991   5753       11ap20714_4692_f3_30   992   5754       hp6p12158_3917638_c1_2   993   5755       09ap10614orf1   994   5756       hp5e25328_3917638_c3_138   995   5757       32236462   9561   9673       423131   9562   9674       14455461   9563   9675       26306340   9564   9676       23531562   9565   9677       A.3.4 Involved in secretion &amp; adhesion       hp3e10946orf2   996   5758       hp3e10946_7157843_f2_2   997   5759       hp3e10946orf1   998   5760       hp3e10946_2226010_f1_1   999   5761       hp3e10946_7157843_f2_2   1000   5762       hp4e13394_26750068_c3_113   1001   5763       197166   1002   5764       11ae80818_197166_c3_63   1003   5765       03ap21820orf13   1004   5766       06cp30603orf16   1005   5767       06cp30603_23452_c3_80   1006   5768       09cp10713_23452_c3_195   1007   5769       24417212   9566   9678       A.3.5 Proteins involved in regulation       01xe21717orf1   1008   5770       01xe21717orf8   1009   5771       01xe21717orf7   1010   5772       01xe21717orf17   1011   5773       05ep10815_204827_c2_92   1012   5774       01xe21717orf2   1013   5775       26261040   1014   5776       01xe21717orf18   1015   5777       05ep10815_21566387_c3_104   1016   5778       03ce11207_23852058_c3_270   1017   5779       hpy09cp10713orf_0002.aa   1018   5780       hpy09cp10713orf_0005.aa   1019   5781       hpy09cp10713orf_0004.aa   1020   5782       09cp10713orf27   1021   5783       09cp10713orf32   1022   5784       09cp10713_21915943_c3_34   1023   5785       09cp10713_21915943_c3_33   1024   5786       32422343   1025   5787       02ae11612_32422343_c2_83   1026   5788       02ae11612orf25   1027   5789       A.3.6 Chaperones       06ep10306orf12   1028   5790       24651083   1029   5791       03ae10804_13862626_c3_45   1030   5792       06ep10306orf3   1031   5793       30089217   9567   9679       A.3.7 Proteins involved in cell division       01ce11016orf13   1032   5794       11cp11224orf4   1033   5795       hpy11cp11224orf_0001.aa   1034   5796       hp6p80503_20051588_c3_36   1035   5797       19626250   1036   5798       02ge10116_22704567_c3_33   1037   5799       02ge10116_22704567_c3_159   1038   5800       02ge20116orf22   1039   5801       hp6p10723orf8   1040   5802       06ap11119_33259403_f3_17   1041   5803       12gp31106_958182_f3_39   1042   5804       13ap11517orf5   1043   5805       06gp71906_24882783_c2_156   1044   5806       12gp31106_24902212_f1_12   1045   5807       13ap11517orf9   1046   5808       04ge11713orf2   1047   5809       04ge11713orf11   1048   5810       04cp11202_956260_f3_35   1049   5811       06gp10920orf3   1050   5812       hp6p10606_26851452_c1_22   1051   5813       hp8e10080_26851452_c1_66   1052   5814       22704567   9568   9680       24427340   9569   9681       A.3.8 Proteins involved in motility       06ep30223_12921892_f1_35   1053   5815       hp3e11024orf14   1054   5816       A.3.9 Other inner membrane proteins       hpy11cp12002orf_0003.aa   1055   5817       hp7p10290_24223782_c2_22   1056   5818       1365943   1057   5819       hp5e12982_1365943_c1_13   1058   5820       hp5e12982orf14   1059   5821       02ge10116_431293_f2_45   1060   5822       hpyhp5e15276orf_0004.aa   1061   5823       02ge11810_67_f1_3   1062   5824       4339708   1063   5825       hpy06ee10709orf_0007.aa   1064   5826       06ee10709orf16   1065   5827       06ee10709_4339708_c2_15   1066   5828       05ae30220_29406392_c3_119   1067   5829       05ae20220orf125   1068   5830       hpyhp3e11168orf_0013.aa   1069   5831       24039587   1070   5832       hp3e11168orf15   1071   5833       04gp11701_24039587_f2_12   1072   5834       1385937   1073   5835       05ep10815_25415887_c2_94   1074   5836       01xe21717orf5   1075   5837       01ae11010_33593_c2_51   1076   5838       hpyhp4e14522orf_0005.aa   1077   5839       05ep10815_34179577_c1_78   1078   5840       34179577   1079   5841       01xe21717orf12   1080   5842       01ae11612_6017137_f3_10   1081   5843       hp6e10180_6921933_f2_2   1082   5844       29ep10720_17089217_f3_18   1083   5845       17089217   1084   5846       11ge10309orf18   1085   5847       23439633   1086   5848       04ep41903_23439633_c2_31   1087   5849       09ae11601orf14   1088   5850       05ae20220orf35   1089   5851       hp5p15870_14350428_f1_1   1090   5852       05ae20220orf56   1091   5853       05ae20220orf10   1092   5854       05ae30220_14350428_f1_9   1093   5855       hpy05ae10307orf_0002.aa   1094   5856       05ae30220_24220937_c2_135   1095   5857       24798427   1096   5858       05ae30220_24798427_f3_52   1097   5859       05ae20220orf31   1098   5860       05ae30220_4708337_c3_116   1099   5861       4708337   1100   5862       05ae20220orf88   1101   5863       23438840   1102   5864       12ae11404_23438840_c1_10   1103   5865       12ae11404orf12   1104   5866       hpy03ae10516orf_0006.aa   1105   5867       33476715   1106   5868       03ae10516orf11   1107   5869       06ap30322_4726503_c2_27   1108   5870       06ap11119_218_f3_18   1109   5871       hp6p10723orf2   1110   5872       06ap11119_26351567_f1_6   1111   5873       26351567   1112   5874       hp6p10723orf13   1113   5875       06ap11119_4726068_f1_5   1114   5876       hp6p10723orf12   1115   5877       06ap11119_4744128_c3_65   1116   5878       4744128   1117   5879       hp6p10723orf43   1118   5880       06cp11118_789033_f3_11   1119   5881       01ce11513orf9   1120   5882       hp6e10967_33986087_c3_25   1121   5883       33986087   1122   5884       hp2p10625orf14   1123   5885       06ep11202_36562767_c3_32   1124   5886       06cp20302orf11   1125   5887       15126875   1126   5888       12gp31106_15126875_c3_68   1127   5889       13ap11517orf31   1128   5890       23473437   1129   5891       14cp10119orf14   1130   5892       hpy14cp10119orf_0001.aa   1131   5893       04cp11202_1953337_f3_49   1132   5894       06gp71906_26567586_f1_14   1133   5895       hpy04ge11713orf_0011.aa   1134   5896       2111040   1135   5897       07ae11008_1307312_c3_58   1136   5898       09cp10502orf14   1137   5899       hpy09cp10502orf_0012.aa   1138   5900       09cp10502orf12   1139   5901       14gp11820orf13   1140   5902       hpy14gp11820orf_0011.aa   1141   5903       07ae11008_14494077_c2_47   1142   5904       09cp20502_14494077_c1_34   1143   5905       23438887   1144   5906       09cp10502orf16   1145   5907       24409577   1146   5908       07ae11008_431591_c1_38   1147   5909       09cp10502orf17   1148   5910       07ae11008_10546918_f1_5   1149   5911       07ae11008_29548890_f3_20   1150   5912       09cp10502orf7   1151   5913       3953952   1152   5914       09cp20502_3953952_c1_33   1153   5915       14gp11820orf4   1154   5916       30730068   1155   5917       07ae11008_4484500_c2_52   1156   5918       09cp10502orf22   1157   5919       14ap10815_23631317_c3_27   1158   5920       23631317   1159   5921       hp3e10349orf25   1160   5922       14cp20718_25812655_c3_20   1161   5923       07ap61111_24744430_c3_112   1162   5924       29zp10241orf14   1163   5925       07ap61111_9776562_c2_86   1164   5926       9776562   1165   5927       14cp20718_9776562_c1_14   1166   5928       13865928   1167   5929       14ee41924_13865928_c1_71   1168   5930       02ep30607orf31   1169   5931       14ee41924_26833437_f2_36   1170   5932       30100332   1171   5933       hp2e10911_31363125_c2_92   1172   5934       hp2e10911_24744076_c1_81   1173   5935       hp2e10911orf30   1174   5936       07ee50709_16601067_f1_28   1175   5937       hpy07ce10203orf_0013.aa   1176   5938       hpy07ce10203orf_0012.aa   1177   5939       hpy07ce10203orf_0014.aa   1178   5940       07ce10203orf22   1179   5941       02ae31010_16679640_f2_21   1180   5942       23526667   1181   5943       02ae31010_24395801_f1_8   1182   5944       07ee50709_16679640_f3_60   1183   5945       207817   1184   5946       29ge30321_207817_f2_8   1185   5947       01ce11618orf10   1186   5948       hp5p15641_23945317_f3_13   1187   5949       23945317   1188   5950       hp5p15641orf9   1189   5951       1071890   1190   5952       02ae31010_4719050_c2_83   1191   5953       02ce11022orf7   1192   5954       598933   1193   5955       hp1p13852orf5   1194   5956       06cp11217_6540927_f1_7   1195   5957       07ge11717_6540927_c1_16   1196   5958       09ce10413_33257312_c3_25   1197   5959       09cp10224_3946875_f1_2   1198   5960       hp1p11244orf10   1199   5961       26758437   1200   5962       14ap11617_26758437_c2_10   1201   5963       05ap11505orf10   1202   5964       1364378   1203   5965       09ze10333_24484777_c1_14   1204   5966       03ge10505orf14   1205   5967       09cp10713_3984818_c2_28   1206   5968       13ae10610_26054808_c1_25   1207   5969       13ae10610_6097567_c3_36   1208   5970       12ee10904orf1   1209   5971       hpy12ee10904orf_0001.aa   1210   5972       14gp11423orf3   1211   5973       14gp11423_24407165_c2_21   1212   5974       14gp11423_35667791_c1_13   1213   5975       14gp11423orf4   1214   5976       14gp11423_19812527_c3_95   1215   5977       12694087   1216   5978       12ae10622_24004137_f3_33   1217   5979       04ep71403orf12   1218   5980       12ae10622_259665_f2_18   1219   5981       259665   1220   5982       12ae10622orf9   1221   5983       32663212   1222   5984       07ce11019_26596881_c1_12   1223   5985       07cp21714orf14   1224   5986       12ge10321_36362687_f2_11   1225   5987       06gp11920orf8   1226   5988       04ep71403orf10   1227   5989       34194093   1228   5990       50062   1229   5991       04ep71403orf15   1230   5992       12ae10622_50062_f3_32   1231   5993       24222885   1232   5994       hp3e10342_24222885_c3_18   1233   5995       01ge11619_24222885_c3_16   1234   5996       11ge10309orf14   1235   5997       05ep21124_189017_f1_1   1236   5998       hp3e11188_189017_c1_19   1237   5999       hp4e13394_24411011_f2_27   1238   6000       24411011   1239   6001       hp4e13394orf5   1240   6002       04ep10206orf5   1241   6003       hp1e13852_2115918_f1_2   1242   6004       03ee11215_2150290_f3_19   1243   6005       2150290   1244   6006       03ee11215orf20   1245   6007       13704718   1246   6008       hp1e13852_5861458_c1_21   1247   6009       04ep10206orf22   1248   6010       hp4e53394_36131715_f1_14   1249   6011       hp1e13852_4486092_f2_7   1250   6012       4486092   1251   6013       06ae11020orf2   1252   6014       06ce10808orf2   1253   6015       hpy06ce10808orf_0002.aa   1254   6016       hp1p13939_25397327_f3_22   1255   6017       06ep30223_1206675_f1_38   1256   6018       1206675   1257   6019       hp3e11024orf17   1258   6020       12617677   1259   6021       06ep30223_12617677_f3_88   1260   6022       14gp12015orf14   1261   6023       hpy02ae20515orf_0001.aa   1262   6024       hpy02xp10215orf_0001.aa   1263   6025       hpy02xp10215orf_0003.aa   1264   6026       07ee30411_9797156_f1_1   1265   6027       06ep30223_5078388_c2_136   1266   6028       01ce11104_11878127_c1_9   1267   6029       16ep10117orf6   1268   6030       26052137   1269   6031       02ae11612_20118768_f2_28   1270   6032       05cp11911orf15   1271   6033       21511555   1272   6034       02ae11612_21511555_f1_18   1273   6035       05cp11911orf13   1274   6036       02ae11612_23437502_f3_39   1275   6037       23437502   1276   6038       02ae11612orf14   1277   6039       01ce11104_36134661_c2_11   1278   6040       36134661   1279   6041       16ep10117orf7   1280   6042       03xe11215orf5   1281   6043       02ae11612_3933437_c3_96   1282   6044       01ce21104_3933437_c2_78   1283   6045       3933437   1284   6046       hp6e10264_2534579_c1_3   1285   6047       hp6e30264_24022187_c2_6   1286   6048       hp5e15084orf5   1287   6049       hp6e10491_17038885_f2_7   1288   6050       hp6e40491_36329178_f1_1   1289   6051       35360843   1290   6052       hp6p10509_21540625_f3_7   1291   6053       16ae10508orf3   1292   6054       24413512   1293   6055       12ap10605_4491302_c1_6   1294   6056       29gp10119orf7   1295   6057       20976500   1296   6058       07ep11916_20976500_f1_8   1297   6059       hp6p12244_20976500_f2_21   1298   6060       01cp11414orf10   1299   6061       hpy02ae11211orf_0001.aa   1300   6062       hp6p12244_34609662_f1_1   1301   6063       34172639   1302   6064       hp6p12244_5869768_f2_24   1303   6065       hp1p13922orf1   1304   6066       hp6p22217_25398250_f3_16   1305   6067       25398250   1306   6068       13ee12016orf18   1307   6069       01ae11421orf4   1308   6070       hpy01ae11421orf_0001.aa   1309   6071       01ae11421orf3   1310   6072       13ae10712_14100018_f2_12   1311   6073       14cp10705orf4   1312   6074       13ae10712_5109376_f3_18   1313   6075       06ge20501_14100018_c1_34   1314   6076       11ae12004_14100018_c1_32   1315   6077       4728193   1316   6078       02gp20814_4728193_f1_3   1317   6079       14ee10308orf9   1318   6080       hpy07ge20415orf_0019.aa   1319   6081       10745275   1320   6082       07ge20415orf30   1321   6083       hp7e10429_22141052_c3_41   1322   6084       5993958   1323   6085       hp7e10429_24023462_c2_36   1324   6086       07ge20415orf39   1325   6087       hp7p90421_23477042_c2_43   1326   6088       33218912   1327   6089       hp3p10349orf32   1328   6090       02ce41018_33367812_f2_14   1329   6091       24078837   1330   6092       07ap20216_24078837_f1_4   1331   6093       05ap21216orf7   1332   6094       hp1p14013orf16   1333   6095       02cp10615orf1   1334   6096       hp1p14013orf13   1335   6097       hpyhp1p14013orf_0015.aa   1336   6098       hp1p14013_21507007_c2_19   1337   6099       hp8e10080_16267837_c3_92   1338   6100       02cp10615_6515756_f1_1   1339   6101       hpyhp1p14013orf_0013.aa   1340   6102       663530   1341   6103       hp1p14013orf4   1342   6104       hp1p14013_4556713_f3_11   1343   6105       03gp20123orf2   1344   6106       hpy03gp20123orf_0002.aa   1345   6107       hpyhp4p12360orf_0001.aa   1346   6108       09cp61003_13710936_c1_73   1347   6109       hp7p10290_24226550_f1_4   1348   6110       hp3p10156orf11   1349   6111       hp5e11634orf2   1350   6112       03ep12017orf1   1351   6113       11cp12002orf7   1352   6114       hp7p10290_244843_c1_16   1353   6115       12ge10305orf3   1354   6116       12ge10305orf4   1355   6117       hp6e12267_32424058_f2_22   1356   6118       hp2p10272orf6   1357   6119       hp2p10272orf3   1358   6120       hp2p10272_23880380_f2_11   1359   6121       06ge10115orf10   1360   6122       hpy06ge10115orf_0011.aa   1361   6123       02gp20706_14494561_c1_37   1362   6124       02gp20706_23535062_f1_2   1363   6125       01cp11710orf7   1364   6126       02gp20706_5314192_f2_11   1365   6127       01cp11710orf12   1366   6128       12969218   1367   6129       02ge11622_23494043_f1_6   1368   6130       13ee10216orf5   1369   6131       hpyhp2e11875orf_0004.aa   1370   6132       06gp11209orf3   1371   6133       hpy06gp11209orf_0001.aa   1372   6134       hpy01ce10320orf_0009.aa   1373   6135       01ce10320_10742802_f1_5   1374   6136       01cp20708_19542968_c1_46   1375   6137       14ee10419orf19   1376   6138       hpy06ep10822orf_0002.aa   1377   6139       06ep10822orf1   1378   6140       hpy06ep10822orf_0001.aa   1379   6141       hp2e11875orf4   1380   6142       01ae11010_29308578_c1_38   1381   6143       14ce31519_1299140_f1_4   1382   6144       02ce10809orf1   1383   6145       04ep41903_23437543_c3_40   1384   6146       02cp10815orf1   1385   6147       09ae11601orf2   1386   6148       04ep41903_5351512_f2_13   1387   6149       23867207   1388   6150       hp5p15212_13095752_c3_36   1389   6151       02ce10213orf23   1390   6152       hpy07ee20513orf_0004.aa   1391   6153       07ee20513orf1   1392   6154       07ee20513orf12   1393   6155       06ae11016_15634702_f2_7   1394   6156       09ae10512_15634702_f3_15   1395   6157       07ee20513orf10   1396   6158       09ae10512_6929702_f1_5   1397   6159       06ae11016_6929702_f3_27   1398   6160       13ee10424orf2   1399   6161       06ap10305orf2   1400   6162       06ep10615_1230462_c3_105   1401   6163       13ee10424_1230462_f3_4   1402   6164       hpy07ge11107orf_0001.aa   1403   6165       11ee11718orf1   1404   6166       06ep10615_16226577_c1_67   1405   6167       06gp10409_33337812_f2_10   1406   6168       04ge11613orf2   1407   6169       06gp10409_5204827_f3_15   1408   6170       06gp10409orf4   1409   6171       04cp11202_19532827_c3_111   1410   6172       04ge11713orf38   1411   6173       04cp11202_29938885_f3_33   1412   6174       04cp11202orf3   1413   6175       hpy04cp11202orf_0001.aa   1414   6176       06gp71906_24352311_c3_199   1415   6177       hpy04cp11202orf_0003.aa   1416   6178       04ge11713orf16   1417   6179       hpy04ge11713orf_0001.aa   1418   6180       04cp11202orf1   1419   6181       04cp11202_25394506_f2_14   1420   6182       hpy02cp11822orf_0010.aa   1421   6183       02cp11822orf4   1422   6184       02cp11822orf3   1423   6185       01ae12021orf1   1424   6186       hpy01ae12021orf_0001.aa   1425   6187       02cp11822orf8   1426   6188       04cp11202_4899192_f2_29   1427   6189       06gp71906_4899192_c3_210   1428   6190       04cp11202_4899192_f3_44   1429   6191       hp5p15861_12698442_f1_3   1430   6192       02cp20821orf12   1431   6193       hp5p15861_14557666_f3_13   1432   6194       02cp20821orf9   1433   6195       14ee41924_12698442_f2_35   1434   6196       hp2e10911orf46   1435   6197       07ap11213_21534415_c3_34   1436   6198       01ce11618orf19   1437   6199       11ae10212orf4   1438   6200       29ge30321_34569437_f2_9   1439   6201       hp2e10911_34569437_c2_85   1440   6202       29ge30321_470158_f1_2   1441   6203       01ce11618orf23   1442   6204       11ep11915orf1   1443   6205       hpy11ep11915orf_0001.aa   1444   6206       hp6p10904_1297062_c3_24   1445   6207       01ee20804orf3   1446   6208       09cp10224_25394752_c1_42   1447   6209       09cp10405orf4   1448   6210       hp4e13394_6854542_c3_114   1449   6211       hp1e13852_6285437_c3_39   1450   6212       04ep10206orf16   1451   6213       hp1e13852_2915903_c1_22   1452   6214       04ep10206orf23   1453   6215       hp4e53394_2915903_f2_43   1454   6216       20173437   1455   6217       06ep30223_20173437_f1_37   1456   6218       hp3e11024orf16   1457   6219       06ce11002_25391000_f1_2   1458   6220       06ce11002orf5   1459   6221       06ep30223_26444203_c1_118   1460   6222       07gp10713orf1   1461   6223       06ep30223_34180312_f2_65   1462   6224       hp3e11024orf10   1463   6225       06ep30223_3964062_f3_96   1464   6226       hp3e11024orf7   1465   6227       06ce11002orf6   1466   6228       06ce11002orf9   1467   6229       06ce11002orf4   1468   6230       06ce11002_4461718_f1_3   1469   6231       06ep30223_5078517_f3_89   1470   6232       14gp12015orf15   1471   6233       29ae22003orf5   1472   6234       hpy29ae22003orf_0001.aa   1473   6235       01cp31002_19539202_f3_8   1474   6236       hpyhp4e14155orf_0004.aa   1475   6237       hpyhp4e14155orf_0003.aa   1476   6238       06ap11008orf1   1477   6239       hpy06ap11008orf_0001.aa   1478   6240       hp6p10590_22290637_f2_11   1479   6241       12ap10605_22459405_f2_2   1480   6242       29gp10119orf2   1481   6243       hp3e10302orf25   1482   6244       hp6p12129_2847212_f1_1   1483   6245       02ae11211orf7   1484   6246       02ae11211orf2   1485   6247       02ae11211orf10   1486   6248       hp6p12244_3987580_f2_17   1487   6249       02ce11418orf2   1488   6250       01cp11414orf14   1489   6251       hp6p12244_5913592_f1_7   1490   6252       hpy29ge10111orf_0003.aa   1491   6253       29ge10111orf9   1492   6254       06ce20610_26448380_c2_31   1493   6255       12ap11614_24400293_c2_10   1494   6256       12ap11614orf11   1495   6257       hp7e10429_25525302_c2_37   1496   6258       07ge20415orf42   1497   6259       03ap21820orf15   1498   6260       03ap21820orf14   1499   6261       03ap21820orf1   1500   6262       11ae80818_421888_c2_52   1501   6263       02ep20506_23712750_f2_7   1502   6264       01ce11513orf6   1503   6265       hpyhp5e15440orf_0015.aa   1504   6266       hp3p10343orf5   1505   6267       14ee11217orf5   1506   6268       14ee11217orf1   1507   6269       11ap20714_33992032_c1_62   1508   6270       06ap11418_33992032_f2_10   1509   6271       hp1p14013orf1   1510   6272       hp1p14013_24239055_f3_8   1511   6273       hp1p14013orf21   1512   6274       hp1p14013orf15   1513   6275       hp1p14013_34187518_c3_24   1514   6276       4714375   9570   9682       29302003   9571   9683       4726503   9572   9684       40339452   9573   9685       3242337   9574   9686       3385833   9575   9687       19536458   9576   9688       34097707   9577   9689       3360130   9578   9690       2548562   9579   9691       20023400   9580   9692       29531590   9581   9693       23494043   9582   9694       36520792   9583   9695       34109763   9584   9696       907827   9585   9697       12698442   9586   9698       55843   9587   9699       25976418   9588   9700       34573431   9589   9701       3987580   9590   9702       33595708   9591   9703       A.4 Other cell envelope proteins       hpy12ge20305orf_0013.aa   1515   6277       01ce61016_16619192_c3_161   1516   6278       09cp61003_16619192_c2_83   1517   6279       01ce61016_1056562_c3_123   1518   6280       hpy06ge10115orf_0008.aa   1519   6281       02gp20706_1050787_c3_60   1520   6282       06ge10115orf8   1521   6283       02ge10116_15632000_c2_114   1522   6284       23610905   1523   6285       05ep10815_1222937_f2_39   1524   6286       01xe21717orf40   1525   6287       20415937   1526   6288       04cp11202_20415937_f2_25   1527   6289       01ae12021orf7   1528   6290       04ge11713orf28   1529   6291       04ge11713orf35   1530   6292       04ge11713orf36   1531   6293       04cp11202_24256567_c3_117   1532   6294       hp5e15044_4554652_f3_3   1533   6295       hpyhp1p13868orf_0008.aa   1534   6296       07cp10312orf5   1535   6297       hp6p10233_12273302_f1_1   1536   6298       07ce10312_4554652_f3_2   1537   6299       04ae61517_21744091_f3_5   1538   6300       hpy13ap11420orf_0001.aa   1539   6301       04ae61517_12345837_f1_2   1540   6302       04ae61517_12345837_f2_4   1541   6303       11cp12006orf10   1542   6304       11cp12006orf13   1543   6305       hp2e10911_24855312_c1_69   1544   6306       01ce11618orf24   1545   6307       hpy01ce11618orf_0014.aa   1546   6308       29ge30321_34157812_f3_10   1547   6309       hpy01ce11618orf_0008.aa   1548   6310       29ge30321_12913562_f1_1   1549   6311       29ge30321_135253_f2_6   1550   6312       01ce11618orf22   1551   6313       01ce11618orf7   1552   6314       01ce11618orf15   1553   6315       hp2e10911_3349_c1_63   1554   6316       02ae31010_4818967_f3_41   1555   6317       01ce11618orf5   1556   6318       02ae31010_15652187_f1_17   1557   6319       07ee50709_4818967_f2_43   1558   6320       04ap12016_25501501_f1_1   1559   6321       29ep10720_25501501_c2_33   1560   6322       25501501   1561   6323       11ge10309orf63   1562   6324       02ae11612orf23   1563   6325       02ae11612_31453292_c3_97   1564   6326       02ae11612_6536567_c2_81   1565   6327       hp5e25328_6539692_c3_148   1566   6328       02ae11612orf31   1567   6329       hp5p15575_1053590_c1_35   1568   6330       hpyhp5p15575orf_0005.aa   1569   6331       hp5p15575_29300311_c1_29   1570   6332       14ce10720orf3   1571   6333       hp7e10433_5345837_c3_13   1572   6334       hp7e10433_25520337_c2_8   1573   6335       hpy01ee21118orf_0002.aa   1574   6336       hp7e10433_5345837_c2_8   1575   6337       24256572   9592   9704       B. CYTOPLASMIC PROTEINS       B.1 Proteins involved in energy metabolism       09ap20802orf36   1576   6338       hp2p10272_11885836_c1_27   1577   6339       hp6p21623_16491412_f1_1   1578   6340       07gp11909_19665932_c3_16   1579   6341       07gp11909orf3   1580   6342       02ap11117_19665932_c2_62   1581   6343       hp2p10272_23835927_f2_13   1582   6344       09ap20802orf16   1583   6345       07gp11909orf1   1584   6346       07gp11909_993792_f3_8   1585   6347       09ap20802orf15   1586   6348       09ap20802orf7   1587   6349       09ap20802orf6   1588   6350       hp2p10272_26366562_f1_3   1589   6351       02ge11622_24245875_f3_44   1590   6352       13ee10216orf12   1591   6353       4897177   1592   6354       02ge11622_36126312_c1_53   1593   6355       13ee10216orf56   1594   6356       13ee10216orf14   1595   6357       hpy04ep11803orf_0001.aa   1596   6358       04ep11803orf3   1597   6359       02ge11622_4101568_f3_46   1598   6360       06ee10709_10270878_f1_1   1599   6361       06ee10709orf1   1600   6362       hpy06ee10709orf_0001.aa   1601   6363       02ge41622_4102312_f1_17   1602   6364       02ge41622_24803341_f2_38   1603   6365       02ge11622_5271943_f3_45   1604   6366       13ee10216orf13   1605   6367       hpy13ee10216orf_0015.aa   1606   6368       13ee10216orf74   1607   6369       13ee10216orf85   1608   6370       02ge11622_6301_c1_55   1609   6371       02ge41622_6301_c2_71   1610   6372       01ae11010_24220625_f2_13   1611   6373       01ce10320orf1   1612   6374       hpy01ce10320orf_0004.aa   1613   6375       01ce10320orf2   1614   6376       01ce10320_24641662_f2_6   1615   6377       01ce10320_35673442_f1_2   1616   6378       01ce10320orf8   1617   6379       06ae11405orf11   1618   6380       hpy06ae11405orf_0010.aa   1619   6381       02ap10801orf1   1620   6382       05ep10815_26679687_c1_73   1621   6383       03ce11207_5351377_c3_267   1622   6384       09ae11601orf13   1623   6385       04ep41903_3251550_c3_39   1624   6386       05ae30220_26351674_c1_64   1625   6387       hpy05ae20220orf_0043.aa   1626   6388       hp2e10626orf2   1627   6389       hpyhp2e10626orf_0002.aa   1628   6390       05ae30220_22861038_c2_136   1629   6391       05ae30220_10977343_c1_140   1630   6392       hp5p15861_1443817_f3_8   1631   6393       02cp20821orf4   1632   6394       14ee41924_6819555_f1_10   1633   6395       11ce10917orf4   1634   6396       11ce10917orf5   1635   6397       11ce10917orf1   1636   6398       11ce10917orf7   1637   6399       14ee41924_4507757_f3_43   1638   6400       07ee11402_6819555_f1_4   1639   6401       07ap11213_10628313_c2_29   1640   6402       hp2e10911orf26   1641   6403       02ae31010_6651712_f3_40   1642   6404       01ce11618orf4   1643   6405       hpy09ce10413orf_0001.aa   1644   6406       02ce10216orf11   1645   6407       02ce10216orf13   1646   6408       09ce10413_4875018_c1_19   1647   6409       hp1p11244orf6   1648   6410       hp1p11244orf9   1649   6411       hp1p11244orf7   1650   6412       09cp10224_24823437_c2_49   1651   6413       hpy02ep20220orf_0002.aa   1652   6414       12ae10622_20322877_f2_25   1653   6415       hpy05gp11901orf_0010.aa   1654   6416       05gp11901orf17   1655   6417       hpy05gp11901orf_0011.aa   1656   6418       04ee11506orf1   1657   6419       hpy04ee11506orf_0001.aa   1658   6420       05ge20108_25945418_c1_6   1659   6421       11ee11408_24650879_c2_40   1660   6422       hp1e80523_24650879_c1_36   1661   6423       hp4e13394_3391637_c1_70   1662   6424       11ce11603orf18   1663   6425       hpy11ce11603orf_0001.aa   1664   6426       hp4e13394_6125462_c3_116   1665   6427       hpy04ep10206orf_0001.aa   1666   6428       04ep10206orf14   1667   6429       hp1e13852_1178175_c2_31   1668   6430       hp4e53394_1178175_f1_14   1669   6431       hpy03ee11215orf_0013.aa   1670   6432       03ee11215orf26   1671   6433       hpy03ee11215orf_0011.aa   1672   6434       03ee11215orf25   1673   6435       03ee11215_6494193_c2_27   1674   6436       06ap12018orf2   1675   6437       hp3e11024orf35   1676   6438       06ep30223_2079552_c2_131   1677   6439       06ce11002orf1   1678   6440       hpy06ce11002orf_0001.aa   1679   6441       06ce11002_113327_f1_1   1680   6442       06ep30223_23442842_c1_103   1681   6443       06ep30223_24613567_c3_150   1682   6444       hpy06ap12018orf_0001.aa   1683   6445       34099062   1684   6446       06ce11002orf2   1685   6447       06ce11002_34099062_f3_5   1686   6448       01ce21104_203142_c3_86   1687   6449       11ee10118orf2   1688   6450       hpy11ee10118orf_0001.aa   1689   6451       hp5e25328_14261087_c1_80   1690   6452       11ge10308_5256_f2_1   1691   6453       11ge10308orf1   1692   6454       hpy11ge10308orf_0001.aa   1693   6455       hp6p12158_21698387_f3_1   1694   6456       hp7e10557_21698387_f1_1   1695   6457       02ae11612_600280_f3_37   1696   6458       02ae11612orf12   1697   6459       hpy02ae11612orf_0001.aa   1698   6460       hp5e25328_34250033_f2_26   1699   6461       01ce21104_24274137_f1_1   1700   6462       03ee11115_205166_f3_2   1701   6463       03ee11115_25525876_f1_1   1702   6464       03ee11115orf1   1703   6465       hpy03ee11115orf_0001.aa   1704   6466       03ee11115orf2   1705   6467       hp5e25328_4884687_c3_139   1706   6468       hp5p15575_5861593_c1_34   1707   6469       hp5p15575_5861593_c2_45   1708   6470       hp6p10590_34063916_f1_4   1709   6471       02gp20814_5350312_f3_8   1710   6472       14ee10308orf6   1711   6473       07ee11905orf1   1712   6474       hpy07ee11905orf_0003.aa   1713   6475       14ce61516_4303467_f1_4   1714   6476       hp3p10343orf1   1715   6477       hpyhp3p10343orf_0002.aa   1716   6478       11ap20714_33375062_f1_3   1717   6479       hpy13ep12003orf_0005.aa   1718   6480       13ep12003orf18   1719   6481       hp6p10606_24237937_c2_27   1720   6482       hp1p14013_36126562_f1_1   1721   6483       hp1p14013orf5   1722   6484       hp1p14013_3257800_f2_4   1723   6485       hp1p14013orf10   1724   6486       02cp10615_36126562_c3_70   1725   6487       29500075   9593   9705       10737627   9594   9706       B.2 Proteins involved in amino acid       metabolism       6696887   1726   6488       02ge10116_6696887_f2_46   1727   6489       11ae10305orf4   1728   6490       02ge11622_30105343_f2_15   1729   6491       13ee10216orf38   1730   6492       02ge41622_30105343_f2_15   1731   6493       31681556   1732   6494       11gp20904_26212885_c3_33   1733   6495       11gp10904orf27   1734   6496       hpyhp4e14522orf_0001.aa   1735   6497       01ae11010_26344041_c1_44   1736   6498       01ae11010_26353437_c3_57   1737   6499       hp4e14522orf18   1738   6500       01ae11010_788312_c1_43   1739   6501       07cp10312orf19   1740   6502       01ae11010_26353437_c2_28   1741   6503       01xe21717orf13   1742   6504       01xe21717orf21   1743   6505       05ep10815_3228467_c1_81   1744   6506       01cp20708_4568818_f2_12   1745   6507       14ee10419orf6   1746   6508       07ge11504orf9   1747   6509       hpy07ge11504orf_0007.aa   1748   6510       12ge10610orf1   1749   6511       hpy12ge10610orf_0001.aa   1750   6512       05ep10815_4864693_c2_98   1751   6513       hpyhp3p10018orf_0001.aa   1752   6514       09ge10522orf2   1753   6515       hpy09ge10522orf_0002.aa   1754   6516       05ep10815_4901462_f2_35   1755   6517       hpy11ap11819orf_0001.aa   1756   6518       04ep41903_36225883_f2_11   1757   6519       05ae20220orf21   1758   6520       05ae20220orf47   1759   6521       05ae20220orf65   1760   6522       05ae30220_4698842_f2_42   1761   6523       hpy07ee20513orf_0015.aa   1762   6524       07ee20513orf9   1763   6525       09ae10512_4336568_f3_24   1764   6526       06ep11202_4884677_c1_17   1765   6527       06cp20302orf5   1766   6528       hp3e11122orf4   1767   6529       06gp31906orf3   1768   6530       06gp31906orf4   1769   6531       hpy06gp31906orf_0002.aa   1770   6532       06gp71906_31290937_c2_163   1771   6533       06gp71906_37592_f1_27   1772   6534       hp4p11393orf3   1773   6535       hpyhp4p11393orf_0001.aa   1774   6536       hp4p11393orf4   1775   6537       06gp71906_3986502_f3_111   1776   6538       14gp11820orf20   1777   6539       14gp11820orf27   1778   6540       09cp20502_23475342_f2_18   1779   6541       23475342   1780   6542       14gp11820orf7   1781   6543       14gp11820orf3   1782   6544       09cp20502_24613337_c2_43   1783   6545       07ae11008_24613337_c3_55   1784   6546       34189716   1785   6547       29ge30321_34189716_f3_12   1786   6548       01ce11618orf18   1787   6549       09ce52017_4005267_c1_20   1788   6550       01ep30520orf23   1789   6551       24409641   1790   6552       06cp30603_23567890_c1_86   1791   6553       05cp20518orf61   1792   6554       05cp20518orf12   1793   6555       05cp20518orf32   1794   6556       06cp30603_23595967_f2_33   1795   6557       11ge61517_23595967_f1_20   1796   6558       06cp30603_5364452_c3_141   1797   6559       04gp11213orf49   1798   6560       hp4e13394orf3   1799   6561       hp4e13394orf7   1800   6562       hp4e13394_1296888_f2_28   1801   6563       09ap11406orf27   1802   6564       09ap11406orf17   1803   6565       hp4e13394_23470963_f2_29   1804   6566       hp4e13394_24636562_f2_44   1805   6567       06ep11108orf15   1806   6568       06ce10907orf2   1807   6569       09gp10903orf3   1808   6570       hpy09gp10903orf_0004.aa   1809   6571       hp4e13394_25563962_f3_47   1810   6572       06ce10907orf1   1811   6573       hpy06ce10907orf_0001.aa   1812   6574       hp4e13394_26736040_f1_2   1813   6575       09gp10903orf2   1814   6576       09ap11406orf26   1815   6577       09gp10903orf5   1816   6578       hp4e13394_33306533_f1_3   1817   6579       09ap11406orf19   1818   6580       hp4e13394_34171916_f1_4   1819   6581       04ep10206orf13   1820   6582       hp1e13852_23477192_c2_30   1821   6583       hp4e53394_23477192_f1_13   1822   6584       12ge10513orf2   1823   6585       hpy12ge10513orf_0001.aa   1824   6586       03ee11215_7320937_f1_8   1825   6587       03ee11215orf11   1826   6588       hp4e53394_7320937_f1_11   1827   6589       14cp11121orf1   1828   6590       14cp11121orf7   1829   6591       05ce10208_36128141_f3_13   1830   6592       02ae11612_16834692_f1_15   1831   6593       05cp11911orf10   1832   6594       hp4e14535orf7   1833   6595       hp4e14535orf8   1834   6596       hp4e14535orf3   1835   6597       06ep11917_24803153_c3_24   1836   6598       4570262   1837   6599       02ae11612_4570262_f3_38   1838   6600       02ae11612orf13   1839   6601       14ce10720orf4   1840   6602       14ce10720orf1   1841   6603       hp5p15575_34156437_c2_36   1842   6604       05ge11422orf2   1843   6605       05ge11422orf1   1844   6606       hpy05ge11422orf_0001.aa   1845   6607       06ce20610_12925201_c3_35   1846   6608       hp7e10228_4892592_f1_1   1847   6609       hpy14ep11807orf_0001.aa   1848   6610       45914063   1849   6611       12ap11614orf4   1850   6612       hpy12ap11614orf_0010.aa   1851   6613       12ap11614_34469527_f2_3   1852   6614       09cp21607_989055_c1_9   1853   6615       07gp31516orf6   1854   6616       11ae10710orf1   1855   6617       hpy11ae10710orf_0001.aa   1856   6618       hp7p10287_24666030_f2_5   1857   6619       hpyhp3p10349orf_0009.aa   1858   6620       02ce41018_24303276_f3_19   1859   6621       hp3p10349orf23   1860   6622       hp7e10420_24484767_f3_18   1861   6623       06ap20306_22269807_c2_17   1862   6624       04gp11803orf6   1863   6625       03ae11503_4883587_c1_15   1864   6626       11ap20714_4883587_f3_47   1865   6627       03ae11503orf7   1866   6628       02cp11721orf11   1867   6629       hp7e10100_3254805_c3_19   1868   6630       21976637   9595   9707       677088   9596   9708       B.3 Proteins involved in cofactor       metabolism       hp6e12267_1204050_c2_46   1869   6631       12ge10305orf14   1870   6632       hp6e12267_24089437_c3_61   1871   6633       24089437   1872   6634       12ge20305orf14   1873   6635       09ae10323orf2   1874   6636       09ae10323orf4   1875   6637       09cp61003_283442_f1_19   1876   6638       12ge20305orf28   1877   6639       hp6e12267_4298467_f3_30   1878   6640       hp6e12267_4428467_c2_51   1879   6641       12ge20305orf10   1880   6642       hp2p10272_14094708_f2_9   1881   6643       hpyhp2p10272orf_0002.aa   1882   6644       hp6p21623_14882837_f3_24   1883   6645       hp2p10272orf4   1884   6646       hpyhp2p10272orf_0001.aa   1885   6647       02ap11117_14882837_c2_67   1886   6648       hp5e12982_415907_f1_2   1887   6649       hp5e12982orf5   1888   6650       14480927   1889   6651       02ge10116_24417562_c2_26   1890   6652       02ge10116_24417562_c2_123   1891   6653       02ge20116orf33   1892   6654       hp5e15276orf8   1893   6655       02ge10116_4765638_c3_145   1894   6656       02ge10116_9956415_c1_83   1895   6657       04ap10921orf3   1896   6658       hpy04ap10921orf_0001.aa   1897   6659       02ge11810_26359537_f2_5   1898   6660       13ee10216orf42   1899   6661       13ee10216orf8   1900   6662       02ge11622_4897842_f2_19   1901   6663       09ge21419orf1   1902   6664       01ee60820_14183378_f2_18   1903   6665       03ce11207_14183378_f3_150   1904   6666       11gp10904orf14   1905   6667       11gp10904orf13   1906   6668       11gp20904_4414717_f2_13   1907   6669       01ee60820_4414717_f1_6   1908   6670       01ce10320orf6   1909   6671       hpy01ce10320orf_0001.aa   1910   6672       01ce10320_26054633_f3_8   1911   6673       01ce10320orf4   1912   6674       05ep10815orf2   1913   6675       hpy05ep10815orf_0001.aa   1914   6676       05ep10815_7818_c1_89   1915   6677       03ce11207_7818_c1_187   1916   6678       hpy05ae20220orf_0042.aa   1917   6679       05ae20220orf94   1918   6680       05ae30220_24507712_c2_80   1919   6681       02ce10213orf10   1920   6682       02ce10213orf3   1921   6683       hp5p15212_23635441_f3_17   1922   6684       02ce10213orf18   1923   6685       hp5p15212_4687538_f2_7   1924   6686       06ep11202_133293_c1_19   1925   6687       06cp20302orf3   1926   6688       04cp11202_36522218_c3_113   1927   6689       04ge11713orf40   1928   6690       04cp11202_5114015_f3_38   1929   6691       04ge11713orf20   1930   6692       hpyhp2e10911orf_0009.aa   1931   6693       hp2e10911_34173401_c2_90   1932   6694       hpyhp2e10911orf_0008.aa   1933   6695       07ap11213_35156577_c1_24   1934   6696       hp2e10911orf35   1935   6697       07ee50709_35156577_f3_80   1936   6698       09ze10333_4710386_f2_8   1937   6699       03ge10505orf3   1938   6700       09ze10333_4710386_f2_7   1939   6701       1408   1940   6702       09cp10713_30470301_c2_31   1941   6703       09cp10713_30470301_c2_30   1942   6704       09cp10713orf29   1943   6705       12ge10321_6923512_f2_8   1944   6706       hp4p11352orf7   1945   6707       06ep10306orf4   1946   6708       03ae10804_976702_c2_34   1947   6709       06ep10306orf13   1948   6710       hp2e10619orf2   1949   6711       hp2e10619orf1   1950   6712       hp4e13394_16984790_f2_41   1951   6713       hpy11cp10916orf_0002.aa   1952   6714       hp4e53394_4556458_f1_12   1953   6715       hp4e53394_16211062_f3_61   1954   6716       03ee11215_23985910_c3_33   1955   6717       hp4e53394_23985910_c3_117   1956   6718       03ee11215orf34   1957   6719       03ee11215_35960937_f1_6   1958   6720       hp4e53394_35960937_f1_9   1959   6721       03ee11215orf9   1960   6722       01ge10203_16689377_c1_11   1961   6723       01ge10203orf8   1962   6724       05ae11506orf3   1963   6725       01ge10203orf11   1964   6726       hp1p13939_22656457_c2_34   1965   6727       01ge10203_22656457_c2_15   1966   6728       hp4p62853_22656457_c1_35   1967   6729       06ep30223_23557202_c2_130   1968   6730       hp3e11024orf49   1969   6731       hpy02ce11220orf_0001.aa   1970   6732       05ee10411orf5   1971   6733       hpy05ee10411orf_0001.aa   1972   6734       06ep30223_5109443_c1_109   1973   6735       10407625   1974   6736       02ae11612_24335942_c2_84   1975   6737       02ae11612orf26   1976   6738       2040717   1977   6739       02ae11612_32710417_c1_62   1978   6740       02ae11612orf36   1979   6741       hp5e25328_4490967_c2_126   1980   6742       hp7e10434_25394812_f2_8   1981   6743       06ce10515orf2   1982   6744       02ce41018_4976537_f1_7   1983   6745       hp3p10349orf26   1984   6746       02ce41018_35945968_f3_25   1985   6747       hp3p10349orf22   1986   6748       hp7p90421_4976537_f3_27   1987   6749       11ap20714_20494837_f3_42   1988   6750       hpy04gp11803orf_0002.aa   1989   6751       11ap20714_21759681_c1_63   1990   6752       hp5e15440orf25   1991   6753       23442642   1992   6754       06ap11418_23442642_f1_6   1993   6755       hp5e15440orf21   1994   6756       11ap20714_2588387_f3_44   1995   6757       07cp10301orf2   1996   6758       05ap21216orf16   1997   6759       05ap21216orf24   1998   6760       07ap20216_31912575_c2_24   1999   6761       11ap20714_31912575_c2_82   2000   6762       hp3e10057orf1   2001   6763       11ap20714_3228437_f2_28   2002   6764       13ep12003orf3   2003   6765       13ep12003orf12   2004   6766       hp6p10606_23437942_f1_5   2005   6767       485375   9597   9709       B.4 Proteins involved in carbohydrate       metabolism       2855006   2006   6768       02ge11622_13007837_f1_9   2007   6769       13ee10216orf9   2008   6770       14257751   2009   6771       02ge11622_14257751_f3_37   2010   6772       13ee10216orf43   2011   6773       02ge11622_23992687_f2_27   2012   6774       02ge41622_23992687_f1_16   2013   6775       13ee10216orf30   2014   6776       02ge11622_3988912_f3_39   2015   6777       13ee10216orf45   2016   6778       hpy13ee10216orf_0027.aa   2017   6779       02ge11622_683262_f2_25   2018   6780       02ge41622_683262_f1_14   2019   6781       13ee10216orf28   2020   6782       02ge41622_683262_f3_44   2021   6783       04ep41903_12110918_f1_7   2022   6784       14ce11519orf5   2023   6785       14ce11519orf2   2024   6786       09ae11601orf6   2025   6787       04ep41903_4397717_f3_20   2026   6788       14ce11519orf3   2027   6789       04ep41903_5084843_f3_19   2028   6790       04ep41903_5084843_f2_19   2029   6791       09cp10502orf15   2030   6792       07ae11008_24400293_c1_40   2031   6793       07ce11409orf1   2032   6794       hpy07ce11409orf_0001.aa   2033   6795       hp3p10168orf1   2034   6796       hpyhp3p10168orf_0001.aa   2035   6797       14ee41924_19790902_f3_54   2036   6798       14ee41924_24226562_f2_37   2037   6799       07ce11409orf6   2038   6800       06cp11217_34427203_c2_16   2039   6801       hp1p13852orf14   2040   6802       01ep30520orf7   2041   6803       03ge11517orf2   2042   6804       hpy03ge11517orf_0001.aa   2043   6805       09ce52017_5080442_f3_18   2044   6806       09cp10224_22554651_c2_56   2045   6807       09ze10333_5861452_c1_17   2046   6808       hpy03ge10505orf_0005.aa   2047   6809       15807794   2048   6810       06cp30603_23610930_c3_72   2049   6811       05cp20518orf64   2050   6812       06cp30603_33443_f3_32   2051   6813       05cp20518orf27   2052   6814       13ap11119orf1   2053   6815       hpy13ap11119orf_0001.aa   2054   6816       05ap12011orf1   2055   6817       hpy05ap12011orf_0001.aa   2056   6818       07ce11019_23439204_c1_10   2057   6819       14gp11423_25416691_c2_75   2058   6820       hpy09cp10614orf_0001.aa   2059   6821       09cp10614orf3   2060   6822       12ae10622_4492342_f1_16   2061   6823       14gp11423_4492342_c2_74   2062   6824       23912807   2063   6825       hp4e13394_24298127_c2_106   2064   6826       09ap11406orf8   2065   6827       hpy05cp11911orf_0001.aa   2066   6828       02ee10810orf1   2067   6829       hpy02ee10810orf_0001.aa   2068   6830       02ee10810_20179790_f1_1   2069   6831       02ae11612_4328518_f3_42   2070   6832       01ce21104_4328518_f1_6   2071   6833       hp7e10429_34250036_c1_25   2072   6834       hpy12ap11614orf_0006.aa   2073   6835       12ap11614orf3   2074   6836       12ap11614orf2   2075   6837       12ap11614_4562712_f2_2   2076   6838       13723593   9598   9710       24298127   9599   9711       B.5 Proteins involved in nucleic acid       metabolism       09ae10323orf3   2077   6839       09cp61003_4297967_f3_56   2078   6840       09cp61003_6142965_f2_37   2079   6841       09ae10323orf1   2080   6842       hpy13ee10216orf_0008.aa   2081   6843       02ge41622_14469018_f2_25   2082   6844       02ge11622_14726062_f1_4   2083   6845       13ee10216orf17   2084   6846       hp6p31058_35156592_f3_1   2085   6847       02ge41622_14469018_f3_33   2086   6848       3261306   2087   6849       06ee10709_7118818_f3_9   2088   6850       06ee10709orf5   2089   6851       01ae11010_29297936_c3_43   2090   6852       hp3e11075orf4   2091   6853       01ae11010_35581562_c1_37   2092   6854       01ae11010_40963_c1_36   2093   6855       29ee12019orf1   2094   6856       03ce11207_40963_f1_45   2095   6857       04gp11701_4687842_c2_29   2096   6858       hp3e11168orf26   2097   6859       06ap10609orf3   2098   6860       06ap10609orf2   2099   6861       hpy06ap10609orf_0001.aa   2100   6862       06ap10609_3390656_c1_41   2101   6863       06cp10411orf1   2102   6864       06cp10411orf2   2103   6865       06gp71906_16494193_c3_191   2104   6866       14574201   2105   6867       hpy02cp11822orf_0007.aa   2106   6868       02cp11822orf26   2107   6869       04cp11202_4339813_c1_73   2108   6870       04cp11202_4339813_c3_107   2109   6871       14ee41924_14313885_c3_100   2110   6872       14313885   2111   6873       11ce10917orf14   2112   6874       07ce11409orf11   2113   6875       hpy07ce11409orf_0005.aa   2114   6876       07ce11409orf12   2115   6877       14ee41924_5080087_c1_57   2116   6878       01ep11504orf1   2117   6879       01ge10801orf4   2118   6880       01ep11504orf4   2119   6881       01ep11504orf3   2120   6882       hpy01ep11504orf_0001.aa   2121   6883       02ae31010_16828135_f3_32   2122   6884       hp5p15641_24412842_f1_2   2123   6885       hp5p15641orf11   2124   6886       02ae31010_33203166_f1_13   2125   6887       hp1p13947orf9   2126   6888       hpyhp1p13947orf_0007.aa   2127   6889       hp1p13947orf3   2128   6890       02ae31010_5080463_f2_26   2129   6891       14ae20424orf1   2130   6892       04ae61517_5869133_f2_4   2131   6893       hp4p12313orf4   2132   6894       hp4p12313orf3   2133   6895       09cp10224_22554651_c2_51   2134   6896       32144532   2135   6897       06cp30603_10739532_f1_5   2136   6898       05cp20518orf5   2137   6899       04ge10816orf1   2138   6900       02ee10520orf7   2139   6901       02ee10520orf4   2140   6902       hpy02ee10520orf_0005.aa   2141   6903       04ge10816orf3   2142   6904       hpy04ge10816orf_0001.aa   2143   6905       13ae10610_3962568_c3_37   2144   6906       04ge10816orf2   2145   6907       13ae10610_16407276_c2_31   2146   6908       13ae10610_3962568_c1_26   2147   6909       12ge10321_4298468_f3_15   2148   6910       hp4p11352orf10   2149   6911       09cp10405orf3   2150   6912       09cp10405orf1   2151   6913       hpy09cp10405orf_0001.aa   2152   6914       hp4e13394_4119017_c2_95   2153   6915       hpy01ep30520orf_0002.aa   2154   6916       01ep30520orf8   2155   6917       hpy01ep30520orf_0001.aa   2156   6918       06ep30223_23476067_c1_119   2157   6919       06ep30223_23476067_c1_115   2158   6920       06ep30223_23476067_c1_118   2159   6921       6517040   2160   6922       06ep30223_33600817_f2_63   2161   6923       hp3e11024orf24   2162   6924       867183   2163   6925       06ep11917_21522805_c3_25   2164   6926       hp4e14535orf4   2165   6927       hp6p12129_16192812_c2_22   2166   6928       01ep12002_16192812_c1_13   2167   6929       04ep10515orf8   2168   6930       hp2e10692orf3   2169   6931       14ce61516_6025312_f1_6   2170   6932       hpy05ae10808orf_0003.aa   2171   6933       11ae80818_6900292_c3_67   2172   6934       11ae80818_976506_f2_20   2173   6935       hpy06cp11722orf_0004.aa   2174   6936       04ae21413_26360918_f3_2   2175   6937       04ae11413orf3   2176   6938       04ae11413orf1   2177   6939       04ae21413_19532530_f1_1   2178   6940       11ap20714_34663910_f3_29   2179   6941       04ae11413orf2   2180   6942       hp5e15440orf7   2181   6943       hp8e10065_4962812_f2_18   2182   6944       1581937   9600   9712       B.6 Proteins involved in lipid metabolism       hp6e12267_14650278_f3_29   2183   6945       12ge20305orf26   2184   6946       13ee10216orf1   2185   6947       hpy13ee10216orf_0001.aa   2186   6948       02ge11622_26023917_f1_1   2187   6949       02ge41622_33985402_f1_1   2188   6950       hp3e11060orf16   2189   6951       09ae10512_4334717_f1_7   2190   6952       422937   2191   6953       29zp10241orf11   2192   6954       14cp20718_22767327_c3_19   2193   6955       hp5p15861_16579692_f2_7   2194   6956       02cp20821orf2   2195   6957       14ee41924_23834800_f2_32   2196   6958       11ce10917orf10   2197   6959       14ee41924_6672317_f2_30   2198   6960       11ce10917orf8   2199   6961       32705252   2200   6962       hp4p11352orf6   2201   6963       hp4p11352orf2   2202   6964       12ge10321_35445843_f2_7   2203   6965       06ep11917_19539202_c1_21   2204   6966       43490713   2205   6967       06ep11917_5114568_c2_23   2206   6968       hp4e14535orf2   2207   6969       14ce61516_16814158_f1_5   2208   6970       14ce61516_14162751_f3_14   2209   6971       hp2e10692orf2   2210   6972       hp2e10692orf1   2211   6973       02ce21018orf4   2212   6974       02ce41018_24314075_c3_46   2213   6975       11ap20714_4578452_f1_8   2214   6976       07ap20216_4578452_f1_5   2215   6977       05ap21216orf8   2216   6978       hp1p14013_35179683_f2_6   2217   6979       hp1p14013orf3   2218   6980       5138   9601   9713       35445843   9602   9714       B.7 Proteins involved in mRNA translation       and ribosome biogenesis       05ee20322orf13   2219   6981       02ge10116_36464702_f3_70   2220   6982       03ap10105orf2   2221   6983       03ap10105orf1   2222   6984       hpy11gp10904orf_0001.aa   2223   6985       01ee60820_36132778_c3_43   2224   6986       03ce11207_36132778_c2_225   2225   6987       785437   2226   6988       01ae11010_5192143_c3_32   2227   6989       hp4e14522orf11   2228   6990       05ep10815_5318766_c1_87   2229   6991       07ge11504orf7   2230   6992       11gp10904orf24   2231   6993       11gp20904_6136000_c2_29   2232   6994       11gp20904_791318_c3_32   2233   6995       11gp10904orf28   2234   6996       05ae30220_14647183_c2_83   2235   6997       05ae20220orf72   2236   6998       05gp11901orf27   2237   6999       05gp11901orf21   2238   7000       hp5p15496orf1   2239   7001       11ee11408_3955010_c1_36   2240   7002       04ee11108_4801318_f1_8   2241   7003       27ze10351orf6   2242   7004       06ae11122orf1   2243   7005       hpy06ae11122orf_0001.aa   2244   7006       05ae30801_22556543_c2_19   2245   7007       05ae30801_24792212_c1_16   2246   7008       hpyhp1e10446orf_0002.aa   2247   7009       05ae30801_26306562_c3_23   2248   7010       05ae30801_33204692_c2_21   2249   7011       05ae30801_33290942_c2_17   2250   7012       hp1e10446orf5   2251   7013       05ae30801_34196062_c3_24   2252   7014       05ae30801_4955468_c2_18   2253   7015       14gp11419orf1   2254   7016       24500088   2255   7017       hp5p15212_806540_f2_8   2256   7018       02ce10213orf2   2257   7019       01ae12001_3907688_c2_37   2258   7020       07gp11807orf45   2259   7021       hp6e10967_4506518_c1_16   2260   7022       hp2p10625orf24   2261   7023       06ep11202_3956713_c2_25   2262   7024       14ge10402orf1   2263   7025       02cp11822orf23   2264   7026       02cp11822orf18   2265   7027       04cp11202_4490968_c2_78   2266   7028       04cp11202_4490968_c3_113   2267   7029       hpy02cp11822orf_0004.aa   2268   7030       4895327   2269   7031       04cp11202_4895327_c1_74   2270   7032       04cp11202_4895327_c3_108   2271   7033       02cp11822orf22   2272   7034       06gp71906_4895327_f3_94   2273   7035       07ae11008_24023588_c2_42   2274   7036       hp5e15003orf1   2275   7037       hp5p15003orf2   2276   7038       hpyhp5p15003orf_0001.aa   2277   7039       14gp11820orf5   2278   7040       hpy14gp11820orf_0001.aa   2279   7041       07ae11008_26258557_c3_52   2280   7042       09cp20502_26258557_c2_41   2281   7043       14gp11820orf2   2282   7044       14gp11820orf11   2283   7045       09cp20502_4562512_c1_32   2284   7046       hp5e15003orf2   2285   7047       07ae11008_6680318_c2_41   2286   7048       14cp20718_24250207_c3_18   2287   7049       29zp10241orf13   2288   7050       hpy07ap11111orf_0003.aa   2289   7051       07ap11111orf20   2290   7052       hpy07ap11111orf_0001.aa   2291   7053       07ap11111_26172193_c1_13   2292   7054       14cp12009orf5   2293   7055       14cp12009orf2   2294   7056       hpy14cp12009orf_0001.aa   2295   7057       01ap10322orf2   2296   7058       07ap11111_31884687_f2_7   2297   7059       07ap11111orf5   2298   7060       hpy14cp12009orf_0003.aa   2299   7061       14cp12009orf4   2300   7062       07ap61111_31884687_f3_39   2301   7063       07ap61111_31884687_f3_41   2302   7064       14ee41924_16282067_c1_72   2303   7065       02ep30607orf32   2304   7066       02ep30607orf23   2305   7067       14ee41924_19565702_c1_69   2306   7068       07ee11402_19565702_c2_88   2307   7069       hp5p15861_26360952_f3_11   2308   7070       02cp20821orf7   2309   7071       01ce11618orf50   2310   7072       01ce11618orf32   2311   7073       29ge30321_212832_c2_22   2312   7074       07ap11213_22400307_c2_32   2313   7075       hp2e10911orf29   2314   7076       hp5p15641_34101462_f2_8   2315   7077       hp5p15641orf3   2316   7078       07ap11213_4897193_c1_25   2317   7079       hp2e10911orf48   2318   7080       02cp11404orf4   2319   7081       hpy02cp11404orf_0001.aa   2320   7082       02cp11404orf8   2321   7083       07ge11521_2579708_c2_30   2322   7084       02cp11404orf7   2323   7085       07ge11521_16992087_c1_26   2324   7086       07ge11521_16992087_c2_33   2325   7087       33601578   2326   7088       07ge11521_33601578_c3_36   2327   7089       02cp11404orf11   2328   7090       07ge11521_4476568_c3_37   2329   7091       02cp11404orf12   2330   7092       07ge11521_5859713_c2_31   2331   7093       02cp11404orf2   2332   7094       hp1p11244orf8   2333   7095       01ee20804orf4   2334   7096       hpy01ee20804orf_0002.aa   2335   7097       09cp10224_3906717_c2_50   2336   7098       09cp10224_4720437_c2_47   2337   7099       hp1p11244orf1   2338   7100       11ce11005orf1   2339   7101       06cp30603_16523452_c2_95   2340   7102       05cp20518orf16   2341   7103       05cp20518orf26   2342   7104       06cp30603_24886308_f2_15   2343   7105       06cp30603_24886308_f2_27   2344   7106       05cp20518orf17   2345   7107       05cp20518orf2   2346   7108       06cp30603_34178762_f1_3   2347   7109       09cp10713_5194218_c1_25   2348   7110       09cp10713orf21   2349   7111       09cp10713orf28   2350   7112       09cp10713_23852057_c2_30   2351   7113       09cp10713_5194218_c2_29   2352   7114       hpy07ap11112orf_0001.aa   2353   7115       07ap11112orf1   2354   7116       hp3e10946_34175837_f3_3   2355   7117       hp3e10946_32609412_f3_4   2356   7118       hp3p10048orf3   2357   7119       hp4e13394_1209687_c2_94   2358   7120       01ep10216orf5   2359   7121       hpy01ep10216orf_0003.aa   2360   7122       hp4e13394_14554676_f1_19   2361   7123       hp4e13394_3942318_f1_25   2362   7124       06ep11108orf24   2363   7125       hp4e13394_415657_f1_23   2364   7126       06ep11108orf18   2365   7127       07ap11622orf14   2366   7128       06ae11020orf1   2367   7129       07ap11622orf10   2368   7130       hp1e13852_23599087_f1_1   2369   7131       07gp10713orf3   2370   7132       06ep30223_34244037_c2_139   2371   7133       06ep30223_6110937_c2_140   2372   7134       01ee10520orf1   2373   7135       hpy01ee10520orf_0001.aa   2374   7136       06ep30223_4035262_c1_117   2375   7137       hp5e15555_4035262_c3_166   2376   7138       02ae11612_23598811_c1_63   2377   7139       02ae11612orf37   2378   7140       02ae11612_26835305_f3_43   2379   7141       05cp11911orf7   2380   7142       02ae11612_4488967_f2_29   2381   7143       05cp11911orf16   2382   7144       hp3e11086orf9   2383   7145       hp3e11086orf4   2384   7146       hp3e11086orf5   2385   7147       hp5p15575_15900317_f3_23   2386   7148       hp5p15575_23438812_f2_15   2387   7149       14ee11113orf1   2388   7150       hp5p15575_33478126_f2_14   2389   7151       hp5p15575_4886087_f3_25   2390   7152       hp6e10491_25595443_c1_14   2391   7153       hpyhp5e15084orf_0003.aa   2392   7154       hp6e10491_34415816_c1_16   2393   7155       03ee10521orf2   2394   7156       hp6e10491_36385438_c3_21   2395   7157       hp5e15084orf14   2396   7158       04ee30405_3911693_f3_6   2397   7159       hpy16ae10508orf_0010.aa   2398   7160       hpy16ae10508orf_0009.aa   2399   7161       16ae10508orf18   2400   7162       07cp11107orf1   2401   7163       07cp11107orf2   2402   7164       16ae10508orf19   2403   7165       hp6p10509_12695436_c2_15   2404   7166       07ep11916_10973880_c1_19   2405   7167       01cp11414orf1   2406   7168       hp6p12244_34157500_f1_3   2407   7169       02ae11211orf5   2408   7170       07ep11916_4042162_c3_30   2409   7171       01cp11414orf6   2410   7172       hp6p12244_4883452_f3_33   2411   7173       02ae11211orf1   2412   7174       11ae12004_26275300_c3_48   2413   7175       hpy14cp10705orf_0002.aa   2414   7176       hp7e10433_32228441_f2_4   2415   7177       hp5p15504orf8   2416   7178       14ce21516_22394702_f2_2   2417   7179       14ce21516orf2   2418   7180       14ce61516_7031692_f1_1   2419   7181       hp7e10590_33406577_c2_91   2420   7182       06cp11722orf20   2421   7183       11ae80818_3961000_f2_18   2422   7184       25595387   2423   7185       hp7e10434_25595387_f3_16   2424   7186       06ce10515orf4   2425   7187       06ae10902_1209842_f2_2   2426   7188       06ae10902orf1   2427   7189       hp7p10325_22861641_f2_2   2428   7190       06ae10902_24610937_f3_7   2429   7191       06ae10902orf2   2430   7192       06ae10902_26758380_f3_6   2431   7193       06ap11418_548837_c2_25   2432   7194       hp5e15440orf9   2433   7195       13ep12003orf14   2434   7196       4177212   2435   7197       01ee11621orf6   2436   7198       13ep12003orf11   2437   7199       hp6p10606_1875_f1_10   2438   7200       24803280   9603   9715       32036462   9604   9716       “B.8 Proteins involved in genome       replication, transcription, recombination and       repair”       11719687   2439   7201       hp3p10156orf8   2440   7202       hp7p10290_1994062_f1_1   2441   7203       hp6e12267_23955008_f1_2   2442   7204       12ge20305orf34   2443   7205       hp2p10272_26354187_c2_36   2444   7206       09ap20802orf28   2445   7207       hp2p10272_4899052_c3_46   2446   7208       09ap20802orf34   2447   7209       hp3e10950orf2   2448   7210       02gp20706_32242202_c3_57   2449   7211       hp6p12311_12894031_c3_12   2450   7212       05ep20322orf5   2451   7213       hp6p12311_4110627_c2_11   2452   7214       05ep20322orf3   2453   7215       02ge10116_4110627_f3_70   2454   7216       06ee10709orf11   2455   7217       06ee10709orf18   2456   7218       06ee10709_24228168_c1_12   2457   7219       01cp20708_12289077_f2_13   2458   7220       14ee10419orf7   2459   7221       05ep10815_16305252_c3_111   2460   7222       16305252   2461   7223       07ge11504orf4   2462   7224       05ep10815_16600757_f2_41   2463   7225       01xe21717orf27   2464   7226       01cp20708_203167_c3_61   2465   7227       14ee10419orf14   2466   7228       hp3e11168orf7   2467   7229       hp3e11168orf6   2468   7230       04gp11701_23625010_f3_21   2469   7231       14ee10419orf17   2470   7232       hpy14ee10419orf_0010.aa   2471   7233       hp3e11168orf36   2472   7234       04gp11701_39068_c1_25   2473   7235       14ee10419orf18   2474   7236       01cp20708_39068_c2_52   2475   7237       07ap80601orf9   2476   7238       07ap80601orf4   2477   7239       07ap80601_19726452_f1_3   2478   7240       11ee11408_23603162_c1_38   2479   7241       hp5p15496orf7   2480   7242       11ee11408_7927_c1_37   2481   7243       hp5p15496orf2   2482   7244       07ap80601orf3   2483   7245       07ap80601_24808433_f1_2   2484   7246       07ap80601_976413_f3_9   2485   7247       07ap80601orf2   2486   7248       05ae30220_976413_c3_204   2487   7249       09ae10512_23618812_c3_64   2488   7250       hp3e11060orf6   2489   7251       09ae10512_23860625_c1_41   2490   7252       hp3e11060orf10   2491   7253       09ae10512_24274162_c1_39   2492   7254       hp3e11060orf8   2493   7255       24818802   2494   7256       09ae10512_24818802_c2_49   2495   7257       hp3e11060orf2   2496   7258       03ce21717orf2   2497   7259       hp1p10488orf7   2498   7260       06ae11016_25431251_f2_22   2499   7261       03ae10516orf13   2500   7262       06ap30322_1213427_c1_26   2501   7263       01ae12001_19695883_c2_45   2502   7264       07gp11807orf55   2503   7265       06ap10609_19695883_c1_38   2504   7266       24036302   2505   7267       01ae12001_23625827_c3_54   2506   7268       07gp11807orf35   2507   7269       14ap10221_36615625_c2_3   2508   7270       11ee11408_36615625_c3_52   2509   7271       05gp11901orf26   2510   7272       01ae12001_7320277_c3_53   2511   7273       07gp11807orf34   2512   7274       29ep10720_21677217_f2_9   2513   7275       11ge10309orf29   2514   7276       06ap11119_975187_f3_22   2515   7277       hp6p10723orf6   2516   7278       06cp11118_117325_f3_12   2517   7279       01ce11513orf10   2518   7280       01ce11513orf11   2519   7281       01ce11513orf2   2520   7282       06cp11118_24406312_f2_7   2521   7283       05ap11505orf7   2522   7284       hpy05ap11505orf_0007.aa   2523   7285       11ge10309orf11   2524   7286       14ap11617_2040750_f1_1   2525   7287       hp6e10967_10834443_c3_26   2526   7288       hp2p10625orf15   2527   7289       05ee10816_34258562_f1_2   2528   7290       04ce11617orf11   2529   7291       hpy05ee10816orf_0001.aa   2530   7292       hpy05ee10816orf_0004.aa   2531   7293       05ee10816orf2   2532   7294       05ee10816orf1   2533   7295       06ep10615_34662502_f1_11   2534   7296       06ep10615_34662502_f1_11   2535   7297       04ce11617orf1   2536   7298       hpy04ce11617orf_0001.aa   2537   7299       05ee10816_36199207_f3_14   2538   7300       06ep10615_36199207_f1_12   2539   7301       06ep10615_34662502_f2_26   2540   7302       04ce11617orf26   2541   7303       04ce11617orf27   2542   7304       05ee10816_4687651_c1_22   2543   7305       06cp20302orf9   2544   7306       06ep11202_104706_c3_33   2545   7307       06ep11202_26179002_c1_20   2546   7308       06cp20302orf4   2547   7309       06ep11202_26179002_c3_32   2548   7310       06ep11202_3020312_f1_1   2549   7311       06ep11202orf1   2550   7312       hpy06ep11202orf_0001.aa   2551   7313       hpy14ce20219orf_0004.aa   2552   7314       06gp71906_36125062_f1_1   2553   7315       07ap61111_23992002_c1_66   2554   7316       07cp10312_23992002_f2_5   2555   7317       hpyhp1p13868orf_0007.aa   2556   7318       07ap61111_24406593_c3_108   2557   7319       11cp10113orf5   2558   7320       hpy11cp10113orf_0003.aa   2559   7321       hpy01ap10322orf_0001.aa   2560   7322       07ap61111_25599087_f2_20   2561   7323       14ap10815_5274093_f2_5   2562   7324       hp3e10349orf9   2563   7325       260941   2564   7326       04gp11213orf36   2565   7327       14ap10815_6312_c2_22   2566   7328       hp3e10349orf18   2567   7329       07cp10312_24415633_f1_3   2568   7330       hpyhp1p13868orf_0006.aa   2569   7331       14ee41924_23598962_f2_23   2570   7332       23598962   2571   7333       02ep30607orf10   2572   7334       4882652   2573   7335       hp5p15861_4015682_f1_1   2574   7336       02cp20821orf10   2575   7337       14ee41924_4165950_f2_25   2576   7338       02ep30607orf12   2577   7339       07ee30709_16681432_f2_2   2578   7340       07ae11020orf4   2579   7341       07ee50709_32613307_f1_2   2580   7342       07ae11020orf2   2581   7343       07ee30709_1000_f3_3   2582   7344       07ee50709_36031953_f3_76   2583   7345       32627125   2584   7346       09ce52017_35283337_c2_25   2585   7347       hpy01ep30520orf_0006.aa   2586   7348       hpy01ep30520orf_0007.aa   2587   7349       01ep30520orf20   2588   7350       09ce52017_32627125_c3_30   2589   7351       09ce52017_32627125_c2_25   2590   7352       11ge10309orf51   2591   7353       29ep10720_24495437_f3_22   2592   7354       29ep10720_34553192_f1_7   2593   7355       23440814   2594   7356       hpy11ge10309orf_0031.aa   2595   7357       29ep10720_4099093_c1_23   2596   7358       02ap21102_4099093_c2_30   2597   7359       05ap11505orf1   2598   7360       06cp30603_12375083_c2_57   2599   7361       05cp20518orf51   2600   7362       12ae10622_13863791_f3_16   2601   7363       05cp20518orf56   2602   7364       04gp11213orf23   2603   7365       04gp11213orf6   2604   7366       hp6e20339_23556318_c2_47   2605   7367       hp6e20339_24391525_c2_45   2606   7368       06cp30603_24391525_f3_70   2607   7369       04gp11213orf21   2608   7370       06cp30603_32663437_c1_48   2609   7371       11ge61517_24429687_c2_161   2610   7372       05cp20518orf65   2611   7373       hp6e20339_2523462_c3_56   2612   7374       04gp11213orf4   2613   7375       hp3e10302orf13   2614   7376       06cp30603_33250211_f3_65   2615   7377       11cp10410_33250211_f2_2   2616   7378       hpy04gp11213orf_0023.aa   2617   7379       04gp11213orf27   2618   7380       03gp11402orf1   2619   7381       hpy03gp11402orf_0001.aa   2620   7382       hp6e20339_963963_f2_15   2621   7383       06cp30603_33989092_c1_78   2622   7384       23880087   2623   7385       06cp30603_34174212_c3_71   2624   7386       05cp20518orf63   2625   7387       06cp30603_3942318_c3_155   2626   7388       05cp20518orf55   2627   7389       06cp30603_3942318_c2_61   2628   7390       06cp30603_4881577_f2_17   2629   7391       06cp30603_4881577_f1_5   2630   7392       05cp20518orf19   2631   7393       04gp11213orf16   2632   7394       04gp11213orf26   2633   7395       hp6e20339_10197827_c3_59   2634   7396       hp6e20339_5272277_c2_49   2635   7397       04gp11213orf8   2636   7398       06cp30603_5272277_f1_22   2637   7399       hp6e20339_16188561_c1_41   2638   7400       04gp11213orf24   2639   7401       06cp30603_5351512_f3_73   2640   7402       hpyhp3e10302orf_0011.aa   2641   7403       hpyhp3e10302orf_0010.aa   2642   7404       hpyhp3e10302orf_0007.aa   2643   7405       hp3e10302orf41   2644   7406       hp3e10302orf37   2645   7407       06cp30603_7164010_c2_108   2646   7408       hp6e20339_7241093_c1_42   2647   7409       04gp11213orf25   2648   7410       06cp30603_9781882_f1_17   2649   7411       hp3e10302orf7   2650   7412       04ge10816_4032626_f2_9   2651   7413       hpy12ae10622orf_0005.aa   2652   7414       12ae10622orf15   2653   7415       12ae10622_16129812_c2_60   2654   7416       04ep71403orf11   2655   7417       04ep71403orf14   2656   7418       12ae10622_24305312_f1_8   2657   7419       12ge10321_26597180_f1_4   2658   7420       02ep20220orf9   2659   7421       06gp11920orf9   2660   7422       12ge10321_34094067_f2_12   2661   7423       12ae10622_34094067_f1_15   2662   7424       03ae10804_33782135_c3_44   2663   7425       06ep10306orf2   2664   7426       05cp10102orf3   2665   7427       12ge12015orf2   2666   7428       hpy12ge12015orf_0001.aa   2667   7429       hp3e11188_12620840_f3_14   2668   7430       06ep11108orf19   2669   7431       hp4e13394_4328343_f3_59   2670   7432       hp5e13045_1464455_c1_8   2671   7433       hp4e53394_1464455_c1_85   2672   7434       02ae11611orf3   2673   7435       hp3e10975orf10   2674   7436       hp3e10975orf5   2675   7437       hp3e10975orf12   2676   7438       hp4e53394_22005186_c1_82   2677   7439       hp4e53394_22005186_c1_81   2678   7440       hpyhp3e11182orf_0001.aa   2679   7441       11cp10916orf1   2680   7442       hpy11cp10916orf_0001.aa   2681   7443       hp4e53394_5864393_f2_39   2682   7444       hp4p13376_38937_f3_3   2683   7445       05ce10208_1042133_c1_14   2684   7446       hp1p13939orf11   2685   7447       14ae11614orf7   2686   7448       05ae11506orf1   2687   7449       hpy05ae11506orf_0001.aa   2688   7450       12gp11822orf3   2689   7451       12gp11822_25398512_c2_13   2690   7452       hp1p13939_25398512_c1_26   2691   7453       14ae11614orf5   2692   7454       hpy14ae11614orf_0005.aa   2693   7455       14ae11614orf4   2694   7456       09cp71809orf3   2695   7457       12gp11822_4095312_c1_10   2696   7458       hp3e11024orf4   2697   7459       hp3e11024orf3   2698   7460       06ep30223_12538542_f2_58   2699   7461       hp1e10506orf1   2700   7462       05cp21223_35710908_f2_11   2701   7463       07gp10713orf4   2702   7464       hpy07gp10713orf_0001.aa   2703   7465       06ep30223_1445250_f2_45   2704   7466       06ep30223_16512_c3_160   2705   7467       hp3e10128orf1   2706   7468       02ae11612_26212767_c1_65   2707   7469       02ae11612orf40   2708   7470       16ep10117orf10   2709   7471       01ce11104_4689143_c2_13   2710   7472       hp5e25328_4689143_f3_47   2711   7473       hp5p15575orf10   2712   7474       hp5p15575orf6   2713   7475       hp5p15575_20037506_f3_19   2714   7476       04ee30405_6837792_f2_4   2715   7477       hp5e15084orf12   2716   7478       01gp10401orf6   2717   7479       hp6p10590_26181287_c1_27   2718   7480       hp6p10590_338_c2_34   2719   7481       01gp10401orf3   2720   7482       hp6p12244_23726552_f2_32   2721   7483       hpy14cp10923orf_0001.aa   2722   7484       13ee12016orf16   2723   7485       hpy13ee12016orf_0001.aa   2724   7486       13ee12016orf17   2725   7487       13ee12016orf9   2726   7488       hp6p22217_24507800_f3_10   2727   7489       hpy02ge10814orf_0001.aa   2728   7490       11ae12004_24422692_f1_1   2729   7491       06ce20610orf9   2730   7492       11ae12004_14087833_f3_21   2731   7493       11ae12004orf1   2732   7494       hpy11ae12004orf_0001.aa   2733   7495       06ge10717orf3   2734   7496       06ce20610orf7   2735   7497       11ae12004_26384838_f2_6   2736   7498       06ce20610_26369057_c3_39   2737   7499       hp7e10228_26369057_f1_5   2738   7500       13ae10712_3987531_f2_8   2739   7501       13ae10712orf6   2740   7502       07ge20415orf7   2741   7503       07ge20415orf16   2742   7504       hp7e10429_4960880_f2_16   2743   7505       hpy14ep11905orf_0006.aa   2744   7506       14ep11905orf10   2745   7507       hpy14ep11905orf_0008.aa   2746   7508       hpy14ep11905orf_0005.aa   2747   7509       14ep11905orf7   2748   7510       14ce61516_1250_f1_7   2749   7511       14ce61516_1250_f1_7   2750   7512       14ep11905orf9   2751   7513       hpy14ep11905orf_0001.aa   2752   7514       14ep11905orf13   2753   7515       14ce61516_13073577_f2_12   2754   7516       hp7e10590_13073577_c3_107   2755   7517       14ce61516_25402267_f1_8   2756   7518       14ce61516_12600937_f2_11   2757   7519       14ep11905orf8   2758   7520       hpy14ep11905orf_0003.aa   2759   7521       14cp11908_25402267_c3_104   2760   7522       04ap20904orf3   2761   7523       14ce21516orf1   2762   7524       14ce21516_85786_f1_1   2763   7525       07gp31516orf8   2764   7526       07gp31516orf1   2765   7527       09cp21607_33282767_c3_15   2766   7528       07gp31516orf2   2767   7529       07gp31516orf10   2768   7530       09cp21607_42950_c2_13   2769   7531       hp7e30434_42950_f1_9   2770   7532       hp7e10192_992337_f1_3   2771   7533       02ce10114orf2   2772   7534       hpy02cp12005orf_0001.aa   2773   7535       hpy02cp12005orf_0002.aa   2774   7536       05ae10702orf2   2775   7537       05ae10702orf1   2776   7538       05ge11017orf6   2777   7539       05ae10702orf4   2778   7540       05ge11017orf7   2779   7541       02cp12005orf4   2780   7542       hpy02cp12005orf_0003.aa   2781   7543       05ge11017orf5   2782   7544       06ae10902_13087518_f2_5   2783   7545       hp2e11835orf2   2784   7546       hp2p11834orf1   2785   7547       06ae10902_24306512_f2_3   2786   7548       hp2p11834orf2   2787   7549       06ae10902_25394513_f1_1   2788   7550       hp7p10325_24306512_f1_1   2789   7551       02ce41018_4960818_c3_41   2790   7552       hp3p10349orf10   2791   7553       06ap20306_14455043_f3_8   2792   7554       04gp11803orf15   2793   7555       10677187   2794   7556       06ap11418_204202_f1_3   2795   7557       hp5e15440orf18   2796   7558       hpy03ae11503orf_0003.aa   2797   7559       03ae11503orf6   2798   7560       hpy03ae11503orf_0001.aa   2799   7561       03ae11503orf11   2800   7562       03ae11503orf8   2801   7563       03ae11503_21602187_c1_16   2802   7564       07ap20216_33312808_f2_6   2803   7565       05ap21216orf1   2804   7566       hp3p10343orf2   2805   7567       hpyhp3p10343orf_0001.aa   2806   7568       11ap20714_33376087_f2_25   2807   7569       12520952   9605   9717       487750   9606   9718       85786   9607   9719       36523442   9608   9720       B.9 Proteins involved in secretion and       adhesion       hp6e12267_14642202_c2_47   2808   7570       12ge10305orf15   2809   7571       14642202   2810   7572       30662792   2811   7573       14cp10119orf16   2812   7574       34427317   2813   7575       14cp10119orf15   2814   7576       14cp10119orf12   2815   7577       04cp11202_13781712_f2_27   2816   7578       02cp11404orf1   2817   7579       6517192   2818   7580       02cp11404orf9   2819   7581       07ge11521_21494062_c1_27   2820   7582       03ae11503orf9   2821   7583       hp3e10057orf5   2822   7584       hpyhp3e10057orf_0004.aa   2823   7585       03ae11503_24881306_c2_18   2824   7586       B.10 Cytoplasmic proteins involved in outer       membrane &amp; cell wall biogenesis       hpy11gp10904orf_0002.aa   2825   7587       14713512   2826   7588       11gp10904orf29   2827   7589       11gp20904_14713512_c1_27   2828   7590       hp5e15211_11884425_c2_27   2829   7591       hp5e15211orf32   2830   7592       4578469   2831   7593       hp5e15211_4578467_c2_23   2832   7594       hp5e15211orf22   2833   7595       04ee11108_23486342_c2_20   2834   7596       23486342   2835   7597       27ze10351orf22   2836   7598       hpy02ap21113orf_0003.aa   2837   7599       hpy11ep12011orf_0003.aa   2838   7600       02ap21113orf1   2839   7601       hpy11ep12011orf_0004.aa   2840   7602       06gp71906_1257700_c1_124   2841   7603       04ep41903_14729692_f3_15   2842   7604       29ap10306orf2   2843   7605       11253   2844   7606       09ce52017_25890837_c2_24   2845   7607       01ep30520orf27   2846   7608       09cp10224orf6   2847   7609       09cp10224orf3   2848   7610       09cp10224orf7   2849   7611       09cp10224_34180312_c3_64   2850   7612       06cp10117orf1   2851   7613       hpy06cp10117orf_0001.aa   2852   7614       09cp10224_4578292_c3_62   2853   7615       12ae10622_5192075_f2_17   2854   7616       12ae10622orf8   2855   7617       hpy12ae10622orf_0001.aa   2856   7618       05gp10919_11148454_c2_2   2857   7619       05gp10919orf1   2858   7620       hpy05gp10919orf_0001.aa   2859   7621       hp1e80523_1208413_f1_3   2860   7622       02ae11612orf43   2861   7623       02ae11612_12695250_c3_99   2862   7624       05cp11911orf33   2863   7625       02ae11612_24257818_c2_72   2864   7626       06ep11917_4563939_c1_19   2865   7627       hp5e25328_5084843_f1_20   2866   7628       429192   2867   7629       hp6p10509_24304675_c1_13   2868   7630       16ae10508orf21   2869   7631       hpy13ee12016orf_0004.aa   2870   7632       30082267   2871   7633       13ee12016orf10   2872   7634       hp6p22217_33398377_f3_11   2873   7635       hp7e10590_14162751_c1_77   2874   7636       23535937   2875   7637       06cp11722orf15   2876   7638       11ae80818_3322192_f3_37   2877   7639       24441412   9609   9721       34265691   9610   9722       B.11 Cytoplasmic proteins involved in       protein folding and stabilization       hpy05ap11821orf_0001.aa   2878   7640       11ae11815orf1   2879   7641       hpy11ae11815orf_0001.aa   2880   7642       05ap11821orf4   2881   7643       hpy05ap11821orf_0002.aa   2882   7644       09cp61003_4879443_c1_60   2883   7645       hp5e12982orf16   2884   7646       hp5e12982orf13   2885   7647       hpyhp5e12982orf_0001.aa   2886   7648       hp5e12982_32455418_c1_15   2887   7649       hp6p21623_32455418_f2_11   2888   7650       05gp11602orf1   2889   7651       06ap30322orf10   2890   7652       hpy06ap30322orf_0004.aa   2891   7653       06ap30322_24509666_c2_9   2892   7654       06ap30322_4727318_c3_32   2893   7655       hp1p13947orf8   2894   7656       29gp10716orf1   2895   7657       hp1p13947orf1   2896   7658       hpyhp1p13947orf_0001.aa   2897   7659       02ae31010_24408387_f3_35   2898   7660       hp6p10904_260887_c1_19   2899   7661       hp4p13446orf2   2900   7662       hp4p13446orf8   2901   7663       14ep10823orf4   2902   7664       hpy14ep10823orf_0001.aa   2903   7665       hp6p10904_260887_c2_20   2904   7666       09cp10224_24220313_c1_44   2905   7667       hpy09cp10224orf_0002.aa   2906   7668       09cp10224_3932187_c1_45   2907   7669       hp3e11024orf20   2908   7670       hp3e11024orf25   2909   7671       06ep30223_31449217_f3_97   2910   7672       13ae10712_4031500_f3_15   2911   7673       13ae10712orf8   2912   7674       11ae80818_4500342_f2_17   2913   7675       06cp11722orf17   2914   7676       09cp21607_34647812_c1_8   2915   7677       07gp31516orf5   2916   7678       05ce10910_23712780_c1_4   2917   7679       hp7e10192_23712780_f2_5   2918   7680       12343763   9611   9723       6845425   9612   9724       B.12 Other cytoplasmic proteins       hp7p10290_1378917_f3_13   2919   7681       hp3p10156orf5   2920   7682       hp6e12267_2038152_c1_40   2921   7683       12ge20305orf1   2922   7684       09cp61003_23861502_f2_38   2923   7685       11ee10423orf3   2924   7686       hpy11ee10423orf_0001.aa   2925   7687       hp4p12360orf2   2926   7688       09cp61003_25546943_c3_110   2927   7689       hpy01ce11016orf_0004.aa   2928   7690       hpy01ce11016orf_0005.aa   2929   7691       hp6p80503_32462717_f1_5   2930   7692       11cp12002orf3   2931   7693       11cp12002orf2   2932   7694       hp7p10290_35156558_f3_15   2933   7695       hp6p80503_35742151_f3_15   2934   7696       11cp11224orf1   2935   7697       hp6e12267_4095342_f1_4   2936   7698       12ge20305orf21   2937   7699       hp3p10156orf7   2938   7700       hp7p10290_4351718_f1_6   2939   7701       03gp10711orf2   2940   7702       hpy03gp10711orf_0001.aa   2941   7703       09cp61003_4414068_c2_95   2942   7704       hp7p10290_4531713_f2_10   2943   7705       hp3p10156orf1   2944   7706       hp6e12267_4570263_f2_24   2945   7707       12ge10305orf12   2946   7708       01ge11619_1962500_c2_10   2947   7709       14ap11617_32031525_c1_9   2948   7710       01cp10108_1962500_c3_8   2949   7711       hpy05ap11505orf_0003.aa   2950   7712       11ge10309orf3   2951   7713       hp6p21623_22843812_c2_52   2952   7714       hpy09ap20802orf_0020.aa   2953   7715       hp6p21623_24476562_f3_25   2954   7716       hp5e12982_26362717_f1_1   2955   7717       hp5e12982orf4   2956   7718       hp6p21623_33475205_f3_23   2957   7719       hpyhp5e12982orf_0003.aa   2958   7720       hp6p21623_34172075_c2_54   2959   7721       hpy09ap20802orf_0015.aa   2960   7722       hp6p21623_34833_c1_40   2961   7723       hpy09ap20802orf_0011.aa   2962   7724       hp6p21623_35162518_f2_10   2963   7725       hpyhp5e12982orf_0004.aa   2964   7726       01ae10202_4710925_f1_2   2965   7727       11ge10309orf30   2966   7728       04ap12016_4725311_c1_5   2967   7729       29ep10720_4725311_f3_21   2968   7730       02ap21102_4725311_f2_15   2969   7731       11ge10309orf21   2970   7732       02gp20706_3336468_c3_61   2971   7733       02ge10116_3336468_c2_109   2972   7734       06ge10115orf9   2973   7735       02ge10116_10756328_c1_93   2974   7736       hp6p12311_24240708_c1_7   2975   7737       05ep20322orf9   2976   7738       02ge10116_12307812_f3_71   2977   7739       02ge10116_12505125_c3_31   2978   7740       12505125   2979   7741       02ge20116orf20   2980   7742       02ge10116_1376592_f2_50   2981   7743       05ep20322orf7   2982   7744       02ge10116_14182328_f2_29   2983   7745       02ge20116orf2   2984   7746       02gp20706_14507827_c3_65   2985   7747       01cp11710orf38   2986   7748       02ge10116_14649212_c1_20   2987   7749       02ge20116orf26   2988   7750       hpy11ae10305orf_0002.aa   2989   7751       02ge10116_19667752_f3_68   2990   7752       hpy05ee20322orf_0004.aa   2991   7753       hpy05ee20322orf_0002.aa   2992   7754       02ge10116_22854818_f2_47   2993   7755       02ge10116_23650308_f1_24   2994   7756       hpyhp5e15276orf_0003.aa   2995   7757       24070250   2996   7758       02gp20706_24070250_c1_42   2997   7759       02ge10116_24070250_c3_150   2998   7760       06ge10115orf15   2999   7761       02ge10116_26431543_f2_8   3000   7762       02ge20116orf1   3001   7763       02ge10116_31798312_c1_94   3002   7764       hpy02ge20116orf_0011.aa   3003   7765       hp5e15276orf15   3004   7766       02ge10116_33313816_f3_67   3005   7767       02ge10116_36230312_c2_114   3006   7768       hpy01cp11710orf_0013.aa   3007   7769       02ge20116orf7   3008   7770       02ge20116orf13   3009   7771       02ge10116_36369175_f1_1   3010   7772       hpy05ep20322orf_0011.aa   3011   7773       05ep20322orf12   3012   7774       05ep20322orf13   3013   7775       hp6p12311_4689000_f2_3   3014   7776       02ge10116_4689000_c3_130   3015   7777       02gp20706_4767967_f3_21   3016   7778       01cp11710orf3   3017   7779       02ge10116_797180_c2_118   3018   7780       hpy01cp11710orf_0011.aa   3019   7781       02ge10116_977183_f3_52   3020   7782       hpy02ge20116orf_0005.aa   3021   7783       02ge10116_9899142_f3_14   3022   7784       02ge20116orf8   3023   7785       02gp20706_12303811_c3_59   3024   7786       02gp20706_992200_c1_38   3025   7787       06ge10115orf11   3026   7788       06ge10115orf7   3027   7789       02ge10116_992200_c2_107   3028   7790       hp6p12311_9928957_c2_10   3029   7791       02ge10116_9928957_f1_26   3030   7792       05ep20322orf1   3031   7793       02ge11810orf1   3032   7794       hpy02ge11810orf_0001.aa   3033   7795       02ge11810_1457812_f1_1   3034   7796       04ap10921orf4   3035   7797       04ap10921orf6   3036   7798       04ap10921orf1   3037   7799       02ge11810_4532768_f1_2   3038   7800       02ge41622_212825_f1_2   3039   7801       hpy13ee10216orf_0005.aa   3040   7802       13ee10216orf20   3041   7803       13ee10216orf40   3042   7804       02ge11622_23469167_f2_17   3043   7805       02ge11622_24401536_f1_3   3044   7806       13ee10216orf3   3045   7807       03ce10801orf2   3046   7808       hpy06ee10709orf_0009.aa   3047   7809       02ep30607orf4   3048   7810       hpy05ap10120orf_0004.aa   3049   7811       06ee30709_23573392_c3_32   3050   7812       02ge41622_2926257_c3_96   3051   7813       13ee10216orf36   3052   7814       13ee10216orf35   3053   7815       02ge11622_4296877_f3_32   3054   7816       02ge11622_5884392_f2_14   3055   7817       13ee10216orf16   3056   7818       05ae30220_29458178_f3_47   3057   7819       29458178   3058   7820       05ae20220orf51   3059   7821       05ae30220_36132687_f1_4   3060   7822       05ae20220orf4   3061   7823       04ap20412_5250252_c3_1   3062   7824       hp1p10543orf6   3063   7825       07ge11504orf5   3064   7826       07ge11504orf1   3065   7827       hpy07ge11504orf_0001.aa   3066   7828       05ep10815_10972587_c1_85   3067   7829       05ep10815_14273452_c3_105   3068   7830       01xe21717orf3   3069   7831       05ep10815_14646876_f3_63   3070   7832       hpy01xe21717orf_0019.aa   3071   7833       11gp20904_22447333_f1_8   3072   7834       03ce11207_15041083_f1_44   3073   7835       11gp10904orf5   3074   7836       01cp20708_15625202_c3_60   3075   7837       hpy14ee10419orf_0008.aa   3076   7838       11gp20904_23442160_f2_11   3077   7839       11gp10904orf11   3078   7840       hpy06ae11405orf_0001.aa   3079   7841       hpy06ae11405orf_0003.aa   3080   7842       01xe21717orf15   3081   7843       hpy01xe21717orf_0001.aa   3082   7844       hpy06ae11405orf_0002.aa   3083   7845       05ep10815_23845162_c1_75   3084   7846       06ep10822orf5   3085   7847       hp4e14522orf2   3086   7848       01ae11010_23854175_f2_15   3087   7849       01ae11010_23854175_f1_4   3088   7850       01ae11010_23854175_f2_16   3089   7851       02ep10409_23992002_f2_4   3090   7852       01ae11010_23992002_f3_30   3091   7853       07cp10312orf1   3092   7854       hpy09ge10522orf_0001.aa   3093   7855       09ge10522orf1   3094   7856       05ep10815_24042550_f3_60   3095   7857       13ap10114orf1   3096   7858       01cp20708_24235943_f1_9   3097   7859       05ep10815_24406532_f3_64   3098   7860       hpy01xe21717orf_0018.aa   3099   7861       11ap21101_23441308_f2_2   3100   7862       01ae11010_26179686_f2_11   3101   7863       hpy07cp10312orf_0004.aa   3102   7864       01ce10320_26353437_f3_9   3103   7865       01ce10320orf5   3104   7866       05ep10815_26460805_c1_76   3105   7867       01xe21717orf10   3106   7868       29844512   3107   7869       11gp20904_30705212_f2_12   3108   7870       11gp10904orf12   3109   7871       03ae10522orf1   3110   7872       hpy03ae10522orf_0001.aa   3111   7873       05ep10815_32140957_c3_108   3112   7874       03ce11207_32140957_c2_233   3113   7875       hpy01xe21717orf_0014.aa   3114   7876       01xe21717orf43   3115   7877       05ep10815_33237561_f3_66   3116   7878       hpy01xe21717orf_0002.aa   3117   7879       01xe21717orf16   3118   7880       05ep10815_34270136_c3_102   3119   7881       156587   3120   7882       05ep10815_34649002_c2_93   3121   7883       01xe21717orf9   3122   7884       01ae11010_35945827_c1_41   3123   7885       hpyhp4e14522orf_0007.aa   3124   7886       hp3e11170orf3   3125   7887       hp3e11170orf2   3126   7888       hp3e11170orf1   3127   7889       hpyhp3e11170orf_0001.aa   3128   7890       03ce11207orf1   3129   7891       01cp20708_35995718_c3_55   3130   7892       hp3e11168orf28   3131   7893       04gp11701_36114702_c1_24   3132   7894       05ep10815_3949067_c2_91   3133   7895       06ae11405orf7   3134   7896       hpyhp3p10960orf_0001.aa   3135   7897       14ae21813orf1   3136   7898       hpy14ae21813orf_0001.aa   3137   7899       05ep10815_397937_c3_107   3138   7900       01ae11010_42813_f2_10   3139   7901       02ep10409_42813_f1_1   3140   7902       hpy07cp10312orf_0003.aa   3141   7903       01ae11010_5103403_c1_42   3142   7904       01ae11010_5103403_c3_34   3143   7905       hp4e14522orf13   3144   7906       01cp20708_5289051_f3_29   3145   7907       14ee10419orf2   3146   7908       hpy06ae11405orf_0007.aa   3147   7909       06ae11405orf9   3148   7910       05ep10815_24414587_c1_74   3149   7911       03ce11207_5314018_c3_268   3150   7912       01ae11010_5314202_f1_1   3151   7913       07cp10312orf6   3152   7914       01ae11010_26620878_c2_48   3153   7915       29ee12019orf2   3154   7916       hpy29ee12019orf_0001.aa   3155   7917       03ce11207_6525337_f2_101   3156   7918       03cp11207orf1   3157   7919       01cp20708_7070967_c2_48   3158   7920       06ep11502orf1   3159   7921       hpy06ep11502orf_0001.aa   3160   7922       hp5e15211_4494659_c2_26   3161   7923       03gp11902_23473457_f3_12   3162   7924       hp5e15211_35163962_f1_3   3163   7925       35163962   3164   7926       hp5e15211_35163962_f1_3   3165   7927       hp5e15211orf13   3166   7928       hp5e15211_994043_c1_24   3167   7929       hpyhp5e15211orf_0003.aa   3168   7930       01ae11612_16680312_f2_7   3169   7931       07ep11110orf4   3170   7932       01ae11612_22948342_f3_9   3171   7933       07ep11110orf1   3172   7934       01ae11612_30601577_f1_3   3173   7935       07ep11110orf3   3174   7936       29ep10720_23442127_f1_2   3175   7937       06ap10920_33339057_f1_1   3176   7938       11ge10309orf31   3177   7939       07ep11110orf5   3178   7940       01ae11612_4001563_f2_8   3179   7941       hp5e15717_21569141_c1_1   3180   7942       hpy06gp10108orf_0002.aa   3181   7943       02ce10809orf3   3182   7944       02ce10809orf7   3183   7945       14ce31519_236087_f1_5   3184   7946       hp5e15717_24319005_c2_2   3185   7947       hpy06gp10108orf_0001.aa   3186   7948       04ep41903_24319005_c2_57   3187   7949       02cp10815orf3   3188   7950       hpy02cp10815orf_0002.aa   3189   7951       11cp11617orf1   3190   7952       04ep41903_24423963_f1_3   3191   7953       05ae30220_127_c3_177   3192   7954       hpy05ae20220orf_0026.aa   3193   7955       05ae30220_1360633_c3_100   3194   7956       05ae20220orf97   3195   7957       hpy05ap10120orf_0002.aa   3196   7958       03ce10801orf3   3197   7959       hpy05ap10120orf_0003.aa   3198   7960       05ae30220_16519662_c2_128   3199   7961       05ae30220_16519662_c3_204   3200   7962       05ae30220_16523587_f3_69   3201   7963       hpy05ae20220orf_0019.aa   3202   7964       05ae30220_16687512_c1_66   3203   7965       05ae20220orf118   3204   7966       05ae30220_22546878_c3_171   3205   7967       05ae30220_23438156_c2_137   3206   7968       05ae30220_23636252_f2_30   3207   7969       05ae20220orf9   3208   7970       05ae30220_23945442_f3_48   3209   7971       05ae20220orf27   3210   7972       05ae30220_24297042_f3_94   3211   7973       27ze10351orf2   3212   7974       05ae30220_24812811_c1_67   3213   7975       05ae20220orf75   3214   7976       05ae30220_25400312_c2_84   3215   7977       05ae20220orf73   3216   7978       11ee11408_25431467_c2_44   3217   7979       hp5p15496orf4   3218   7980       05ae30220_25587788_f2_29   3219   7981       05ae20220orf8   3220   7982       05ae30220_25656312_c3_102   3221   7983       05ae20220orf99   3222   7984       05ae30220_3240902_f1_11   3223   7985       05ae20220orf13   3224   7986       05ae30220_34022813_c3_185   3225   7987       05ae30220_24492040_f1_2   3226   7988       05ae20220orf2   3227   7989       05ae30220_36618786_f1_2   3228   7990       hpy05ae20220orf_0003.aa   3229   7991       05ae30220_34198517_f2_48   3230   7992       07ap80601_4304687_f2_7   3231   7993       07ap80601orf6   3232   7994       07ap80601orf13   3233   7995       07ap80601_36023958_f3_11   3234   7996       05ae30220_4304687_c1_109   3235   7997       04ee11108_4695468_f3_12   3236   7998       27ze10351orf12   3237   7999       hp5p15496orf6   3238   8000       11ee11408_4881693_c3_53   3239   8001       05ae30220_5283127_f1_10   3240   8002       05ae20220orf12   3241   8003       05ae30220_7265638_f2_35   3242   8004       05ae20220orf38   3243   8005       05ae30220_15719657_f1_1   3244   8006       05ae30220_9881408_f1_1   3245   8007       05ae20220orf1   3246   8008       05ae30220_23551075_f3_71   3247   8009       05ae30801_11753308_f3_13   3248   8010       05ae30801_13869058_c3_26   3249   8011       13ap11204orf6   3250   8012       hp5p15212_13883512_f1_1   3251   8013       02ce10213orf13   3252   8014       14640637   3253   8015       hp5p15212_14641462_f3_14   3254   8016       02ce10213orf15   3255   8017       hp5p15212_23691892_f2_6   3256   8018       02ce10213orf9   3257   8019       hp5p15212_30173405_f3_15   3258   8020       02ce10213orf16   3259   8021       02ce10213orf8   3260   8022       02ce10213orf14   3261   8023       hp5p15212_34172000_f3_13   3262   8024       02ce10213orf17   3263   8025       02ce10213orf1   3264   8026       4531568   3265   8027       hp5p15212_4531568_f3_16   3266   8028       05ee11015orf1   3267   8029       12ae11404_29307332_f3_5   3268   8030       12ae11404orf2   3269   8031       hpy12ae11404orf_0001.aa   3270   8032       05ee11015_13801386_f3_5   3271   8033       12ae11404_24414087_f1_1   3272   8034       12ae11404orf7   3273   8035       09ae10512_12692805_f3_27   3274   8036       hp3e11060orf13   3275   8037       09ae10512_14329791_f3_16   3276   8038       07ee20513orf2   3277   8039       06ae11016_23438577_f2_18   3278   8040       hpyhp3e11060orf_0009.aa   3279   8041       24220627   3280   8042       09ae10512_24220627_f1_3   3281   8043       07ee20513orf14   3282   8044       09ae10512_34023462_f3_26   3283   8045       hp3e11060orf12   3284   8046       07ee20513orf21   3285   8047       hpy07ee20513orf_0010.aa   3286   8048       09ae10512_54187_c2_53   3287   8049       09ae10512_579425_c2_51   3288   8050       hp3e11060orf4   3289   8051       hp3e11060orf11   3290   8052       hpyhp3e11060orf_0012.aa   3291   8053       09ae10512_21642642_c1_42   3292   8054       06ae11016_579425_c2_53   3293   8055       06ae11414_7627_f1_1   3294   8056       hpy11ge10309orf_0033.aa   3295   8057       06ap30322_3956503_c3_34   3296   8058       06ap30322orf11   3297   8059       06ap10322_3956503_c2_34   3298   8060       06ap30322_4415912_f3_23   3299   8061       03ae10516orf3   3300   8062       hpy03ae10516orf_0002.aa   3301   8063       hpy03ae10516orf_0003.aa   3302   8064       06ap30322_781410_f2_13   3303   8065       07gp11807orf38   3304   8066       07gp11807orf51   3305   8067       01ae12001_214812_c3_49   3306   8068       35949212   3307   8069       01ae12001_35949212_c1_36   3308   8070       07gp11807orf44   3309   8071       01ae12001_4882842_c2_44   3310   8072       07gp11807orf33   3311   8073       01ae12001_3335908_c3_51   3312   8074       07gp11807orf41   3313   8075       06ap10609_4882842_c3_54   3314   8076       4882842   3315   8077       07gp11807orf36   3316   8078       14ge10814orf2   3317   8079       hpy14ge10814orf_0003.aa   3318   8080       01ae12001orf1   3319   8081       06ae10609orf1   3320   8082       01ae12001_1070250_c3_55   3321   8083       06ap10609_78527_c3_58   3322   8084       06ap10609_1070250_c1_41   3323   8085       21647676   3324   8086       06ap11119_11892135_f2_16   3325   8087       11ge10309orf15   3326   8088       06ap11119_14253937_c2_42   3327   8089       hpyhp6p10723orf_0021.aa   3328   8090       hpy01ce11016orf_0014.aa   3329   8091       06ap11119_14641700_f2_15   3330   8092       hp6p10723orf21   3331   8093       01ae10202_24095452_f3_3   3332   8094       11ge10309orf16   3333   8095       06ap11119_33205290_f2_12   3334   8096       hp6p1 0723orf18   3335   8097       06ap11119_4879713_f3_24   3336   8098       11ge10309orf26   3337   8099       06ap11119_5112776_c2_43   3338   8100       hpyhp6p10723orf_0020.aa   3339   8101       06ap11119_9797877_f2_13   3340   8102       hp6p10723orf19   3341   8103       12gp11615_4141092_c2_5   3342   8104       02ap21102_4141092_c3_39   3343   8105       hpy11ge10309orf_0002.aa   3344   8106       hp6e10967_19728428_f2_2   3345   8107       hp2p10625orf1   3346   8108       05ee10816_236093_f3_17   3347   8109       04ce11617orf3   3348   8110       06ep10615_23866311_f1_23   3349   8111       06ce10311orf2   3350   8112       06ep10615_24410965_c3_104   3351   8113       06ep10615_32617012_c3_91   3352   8114       hpyhp2p10625orf_0006.aa   3353   8115       13ee10424orf1   3354   8116       hpy13ee10424orf_0001.aa   3355   8117       06ep10615_33416562_c2_82   3356   8118       hpy05ep11717orf_0001.aa   3357   8119       hpy05ep11717orf_0003.aa   3358   8120       hpy05ep11717orf_0006.aa   3359   8121       hpy05ep11717orf_0005.aa   3360   8122       05ep11717orf7   3361   8123       05ep11717orf6   3362   8124       06ep10615_35943787_c2_81   3363   8125       06ep11202_2773375_f3_13   3364   8126       hpy06cp20302orf_0009.aa   3365   8127       06ep11202_4531312_c1_23   3366   8128       hpyhp2e11858orf_0002.aa   3367   8129       hp3e11122orf2   3413   8175       06gp71906_4494063_c2_154   3414   8176       06gp71906_5214068_f1_28   3415   8177       12gp11106orf10   3416   8178       12gp31106_6835827_f1_11   3417   8179       13ap11517orf3   3418   8180       01cp10622_2472312_c2_2   3419   8181       07ae10309_23541676_c3_5   3420   8182       07ae11008_10835762_f1_6   3421   8183       09cp10502orf8   3422   8184       09cp20502_429626_f2_21   3423   8185       14gp11820orf24   3424   8186       07ae11008orf2   3425   8187       07ae11008_4413467_c3_60   3426   8188       29zp10241orf17   3427   8189       14cp20718_23438768_c2_16   3428   8190       07ap11111_23475627_f1_1   3429   8191       07ap11111orf6   3430   8192       29zp10241orf5   3431   8193       14cp20718_23595006_f1_2   3432   8194       07ap11111_2382762_f2_5   3433   8195       23490686   3434   8196       07ap11111orf3   3435   8197       07ap61111_24238327_f2_21   3436   8198       11cp10113orf6   3437   8199       01ae11010_24241562_f1_2   3438   8200       07cp10312orf7   3439   8201       hpy29zp10241orf_0009.aa   3440   8202       07ap61111_24416693_c2_83   3441   8203       11cp10113orf2   3442   8204       07ap61111_24740908_c2_88   3443   8205       26197187   3444   8206       14cp20718_26197187_f2_4   3445   8207       29zp10241orf7   3446   8208       hp6p10233_33397538_f2_9   3447   8209       33397538   3448   8210       hp1p13868orf24   3449   8211       07ap11111_4554637_f1_2   3450   8212       07ap11111orf12   3451   8213       07ap61111_4805338_c1_57   3452   8214       07ap61111_4805338_c2_76   3453   8215       hpyhp3e10349orf_0007.aa   3454   8216       07ap61111_4976512_c2_94   3455   8217       06ap11206_6917518_f3_5   3456   8218       07cp10312orf4   3457   8219       hpyhp3p10180orf_0001.aa   3458   8220       07ap61111_9938162_c1_57   3459   8221       hpyhp3e10349orf_0009.aa   3460   8222       07ce11221_26204752_f1_1   3461   8223       07ce11221orf1   3462   8224       hpy07ce11221orf_0001.aa   3463   8225       14ee41924_11715_f3_53   3464   8226       hpy07ap11015orf_0003.aa   3465   8227       14ee41924_12207031_f1_1   3466   8228       07ee11402_19581265_f3_36   3467   8229       hp5p15861_22697062_f3_9   3468   8230       02cp20821orf5   3469   8231       14ee41924_24334507_f2_24   3470   8232       02ep30607orf11   3471   8233       14ee41924_24351577_c2_86   3472   8234       02ep30607orf13   3473   8235       05ae10307orf2   3474   8236       14ee41924_33477177_f3_52   3475   8237       14ee41924_665937_f3_46   3476   8238       02ep30607orf2   3477   8239       02ae31010_10196928_f2_28   3478   8240       01ce11618orf2   3479   8241       02ae31010_1064125_f1_11   3480   8242       hpy07ce10203orf_0005.aa   3481   8243       02ae31010_4396902_c2_58   3482   8244       07ee50709_1304651_c1_143   3483   8245       07ce10203orf23   3484   8246       07ce10203orf18   3485   8247       02ae31010_1386712_f1_9   3486   8248       02ce11022orf1   3487   8249       hpy02ce11022orf_0001.aa   3488   8250       02ae31010_10835802_f2_18   3489   8251       07ee50709_14632187_f3_77   3490   8252       hp2e10911_16308216_c1_72   3491   8253       hpy01cp11108orf_0003.aa   3492   8254       07ee50709_16308216_f1_20   3493   8255       02ae31010_20991293_c2_73   3494   8256       07ce10203orf7   3495   8257       02ae31010_22539712_f2_20   3496   8258       hp5p15641_23534818_f3_11   3497   8259       hp5p15641orf7   3498   8260       hp5p15641_24804536_f1_4   3499   8261       02ae31010_24898377_f3_37   3500   8262       hp1p13947orf5   3501   8263       02ae31010_250312_f2_24   3502   8264       hpy07ce10203orf_0004.aa   3503   8265       05ap10914orf1   3504   8266       hp5p15641_26367041_c2_30   3505   8267       hpy07ce10203orf_0002.aa   3506   8268       07ce10203orf17   3507   8269       hpy07ce10203orf_0001.aa   3508   8270       02ae31010_2117087_f3_34   3509   8271       02ae31010_26438968_f1_12   3510   8272       07ce10203orf21   3511   8273       07ee50709_26438968_f2_36   3512   8274       01ce11618orf14   3513   8275       02ae31010_34063887_f1_16   3514   8276       02ae31010_4539193_f3_36   3515   8277       hpyhp1p13947orf_0009.aa   3516   8278       hp5p15641_5210892_f2_9   3517   8279       hp5p15641orf4   3518   8280       hp5p15612orf1   3519   8281       13ep10824orf1   3520   8282       hpy13ep10824orf_0001.aa   3521   8283       hp5p15641_6516005_c1_23   3522   8284       02ae31010_828342_f3_38   3523   8285       hp1p13947orf6   3524   8286       882827   3525   8287       02ae31010_882827_f1_15   3526   8288       01ce11618orf20   3527   8289       07ge11521_10739577_c3_34   3528   8290       07ge11521_10739577_c3_36   3529   8291       02cp11404orf5   3530   8292       07ge11521_19536086_c3_40   3531   8293       hpy02cp11404orf_0011.aa   3532   8294       hpy29ap10306orf_0001.aa   3533   8295       07ge11521_26757752_f2_16   3534   8296       02cp11404orf10   3535   8297       07ge11521_4296877_c3_35   3536   8298       06cp11217_22343753_f1_3   3537   8299       hp1p13852orf1   3538   8300       06cp11217_23454382_f1_4   3539   8301       hp1p13852orf2   3540   8302       05ae30220_26054027_c3_176   3541   8303       06cp11217_3907012_f3_12   3542   8304       hp1p13852orf11   3543   8305       06cp11217_4820443_c2_15   3544   8306       hp1p13852orf13   3545   8307       hpyhp4p13446orf_0005.aa   3546   8308       hpyhp4p13446orf_0004.aa   3547   8309       hp6p10904_24218817_f2_7   3548   8310       hp6p10904_3386011_c3_23   3549   8311       hp4p13446orf1   3550   8312       01ep30520orf1   3551   8313       09ce52017_22378887_f1_1   3552   8314       01ep30520orf9   3553   8315       09ce52017_24238312_f2_9   3554   8316       hpy06ep11115orf_0001.aa   3555   8317       09ce52017_25400027_f1_7   3556   8318       06ep11115orf2   3557   8319       09ce52017_5126933_f3_19   3558   8320       09cp10224_10735665_c2_48   3559   8321       hp1p11244orf2   3560   8322       hp6e10735_36515689_c2_1   3561   8323       09cp10224_15908376_c2_46   3562   8324       hp4p13402orf12   3563   8325       09cp10224_24027187_f2_19   3564   8326       09cp10224_24506262_f2_13   3565   8327       01ce10516orf9   3566   8328       09cp10224_32243937_c3_54   3567   8329       hp4p13402orf5   3568   8330       hp1p11244orf15   3569   8331       09cp10224_5078443_f1_6   3570   8332       06cp30603_210042_c3_154   3571   8333       hpy05cp20518orf_0010.aa   3572   8334       hp6e20339_22459406_c2_50   3573   8335       04gp11213orf17   3574   8336       hpyhp3e10302orf_0005.aa   3575   8337       hpyhp3e10302orf_0006.aa   3576   8338       06cp30603_22703965_c3_145   3577   8339       hpy09cp10713orf_0006.aa   3578   8340       09cp10713_22870317_c3_32   3579   8341       09cp10713_23944133_f1_10   3580   8342       06cp30603_26750182_c2_116   3581   8343       hpy05cp20518orf_0017.aa   3582   8344       hp6e20339_30360883_f1_14   3583   8345       19556290   3584   8346       04gp11213orf60   3585   8347       04gp11213orf18   3586   8348       hp6e20339_3162753_c2_51   3587   8349       09cp10713_31798750_c1_26   3588   8350       09cp10713orf24   3589   8351       06cp30603_31798962_c2_103   3590   8352       hpy04gp11213orf_0001.aa   3591   8353       hp6e20339_3253901_c2_44   3592   8354       04gp11213orf20   3593   8355       hp6e20339_32625017_c1_39   3594   8356       04gp11213orf13   3595   8357       hpy05cp20518orf_0024.aa   3596   8358       hpy05cp20518orf_0025.aa   3597   8359       06cp30603_36115877_c2_114   3598   8360       hp6e20339_36620953_c2_48   3599   8361       04gp11213orf7   3600   8362       05cp20518orf36   3601   8363       06cp30603_3906693_c1_42   3602   8364       7031343   3603   8365       09cp10713_7031343_c1_28   3604   8366       09cp10713_7031343_c1_27   3605   8367       09cp10713orf26   3606   8368       06cp30603_9804653_c1_87   3607   8369       hpy05cp20518orf_0023.aa   3608   8370       hp6e52639_21987687_c3_8   3609   8371       01cp11009_23469574_c1_2   3610   8372       hpy05ap11505orf_0006.aa   3611   8373       hpy11ge10309orf_0005.aa   3612   8374       05ee21703_24804712_c1_9   3613   8375       06cp10218_26176689_c1_2   3614   8376       hpy05ap11505orf_0002.aa   3615   8377       13ae10610_5079642_c3_38   3616   8378       02ee10520orf5   3617   8379       06cp30603_24257137_c2_119   3618   8380       32431687   3619   8381       04ep71403orf13   3620   8382       hpy04ep71403orf_0008.aa   3621   8383       12ae10622_34020812_f1_7   3622   8384       12ge10321_34166510_f3_16   3623   8385       hp4p11352orf11   3624   8386       hpyhp4p11352orf_0012.aa   3625   8387       14gp11423_34166510_c2_69   3626   8388       12ge10321_3964812_f1_2   3627   8389       hp4p11352orf3   3628   8390       12ge10321_4040928_f3_13   3629   8391       4040928   3630   8392       hp4p11352orf8   3631   8393       hp4p11352orf5   3632   8394       hp4p11352orf9   3633   8395       12ae10622_4821082_f2_21   3634   8396       03ae10804_10188535_f2_12   3635   8397       06ep10306orf20   3636   8398       06ep10306orf17   3637   8399       hpy06ep10306orf_0017.aa   3638   8400       03ae10804_14878438_c2_38   3639   8401       16251627   3640   8402       03ae10804_1990878_c2_35   3641   8403       06ep10306orf14   3642   8404       03ae10804_21698400_c2_32   3643   8405       06ep10306orf11   3644   8406       03ae10804_26350302_f1_2   3645   8407       hp5e11726orf5   3646   8408       03ae10804_3182663_f1_3   3647   8409       hp5e11726orf6   3648   8410       06ep10306orf8   3649   8411       06ep10306orf9   3650   8412       03ae10804_4507717_c1_25   3651   8413       06ep10306orf16   3652   8414       03ae10804_489413_c3_48   3653   8415       06ep10306orf6   3654   8416       hp1e80523_489413_c3_65   3655   8417       hp5p15006orf1   3656   8418       hpyhp5p15006orf_0001.aa   3657   8419       06ge11904orf2   3658   8420       hpy06ge11904orf_0001.aa   3659   8421       06ee11611orf2   3660   8422       hp3e11188_1379531_f3_13   3661   8423       hp3p10807orf5   3662   8424       hp5e12930orf4   3663   8425       hp5e12930orf6   3664   8426       hp3e11188_26750012_f3_11   3665   8427       hp3p10807_26750012_f3_9   3666   8428       hp3p10807_189075_f2_4   3667   8429       hp3p10807orf6   3668   8430       hpyhp3p10807orf_0001.aa   3669   8431       hp3e11188_47327_f2_5   3670   8432       hp3e11188_47327_f2_9   3671   8433       05ge11120orf1   3672   8434       hp3e11188_910656_f2_10   3673   8435       23441078   3674   8436       hp4e13394_23441078_f1_20   3675   8437       01ep10216orf6   3676   8438       hp4e13394_24735767_f1_22   3677   8439       06ep11108orf22   3678   8440       09cp10405orf2   3679   8441       02ap71220orf1   3680   8442       hp4e13394_36110762_c2_96   3681   8443       09ap11406orf9   3682   8444       09ap11406orf3   3683   8445       hp4e13394_3906592_c2_107   3684   8446       hp4e13394_648452_c3_118   3685   8447       11ce11603orf20   3686   8448       hp4e13394_953883_c2_99   3687   8449       11ce11603orf11   3688   8450       hpy07ap11622orf_0005.aa   3689   8451       01ce10303_9767312_f1_1   3690   8452       hpy04ep10206orf_0004.aa   3691   8453       hp4e53394_15789052_f2_44   3692   8454       03ee11215orf28   3693   8455       03ee11215orf37   3694   8456       03ee11215_16991092_c2_28   3695   8457       03ee11215_22265691_c1_22   3696   8458       22265691   3697   8459       03ee11215orf29   3698   8460       24407533   3699   8461       hp5e13045_24407533_c2_12   3700   8462       02ae11611orf5   3701   8463       hp4e53394_267312_f2_47   3702   8464       hpy07ap11622orf_0003.aa   3703   8465       12ge10513orf1   3704   8466       hpy12ge10513orf_0002.aa   3705   8467       hp4e53394_30203287_f3_58   3706   8468       3157067   3707   8469       03ee11215_3157067_f2_17   3708   8470       03ee11215orf15   3709   8471       hpy03ee11215orf_0003.aa   3710   8472       hpy03ee11215orf_0002.aa   3711   8473       hp4e53394_32618752_c1_81   3712   8474       hp1e13852_4469063_f1_4   3713   8475       04ep10206orf7   3714   8476       03ee11215_5350062_f2_15   3715   8477       03ee11215orf13   3716   8478       hp4e53394_6652327_f1_5   3717   8479       03ee11215orf18   3718   8480       hp5e13045_7055257_c3_15   3719   8481       02ae11611orf2   3720   8482       hp4e53394_8562_f3_65   3721   8483       07ap11622orf1   3722   8484       hp4p13376_13828932_c2_5   3723   8485       hp4p13376_10020149_c1_4   3724   8486       hp2e10350orf1   3725   8487       hp4p13376_26673166_f1_1   3726   8488       05ce10208_14485263_f1_7   3727   8489       14cp11121orf8   3728   8490       14cp11121orf2   3729   8491       05ce10208_23671877_f2_10   3730   8492       hp4p62853_23671877_c1_41   3731   8493       06ce10808orf1   3732   8494       hpy06ce10808orf_0001.aa   3733   8495       hp1p13939_33209817_c2_30   3734   8496       12gp11822_36125062_c2_12   3735   8497       14ae11614orf6   3736   8498       15824052   3737   8499       06ep30223_15824052_f2_54   3738   8500       14gp12015orf12   3739   8501       hpy02ce30710orf_0003.aa   3740   8502       06ep30223_22272187_c2_137   3741   8503       02ce30710orf4   3742   8504       06ep30223_26757775_f3_93   3743   8505       hp3e11024orf13   3744   8506       02ce30710orf1   3745   8507       06ep30223_29736011_c1_114   3746   8508       hp5p10786orf1   3747   8509       06ep30223_30500957_c2_135   3748   8510       7225666   3749   8511       01ep30520orf16   3750   8512       06ep20223_35197255_c2_1   3751   8513       hp3e11024orf11   3752   8514       06ep30223_36125005_f2_66   3753   8515       hp5e15555_36125005_f3_95   3754   8516       06ep30223_35197255_c2_142   3755   8517       hp5e15555_3910937_c2_141   3756   8518       4062813   3757   8519       hp3e11024orf15   3758   8520       06ep30223_4062813_f3_95   3759   8521       hp3e11024orf6   3760   8522       06ep30223_4062813_f1_22   3761   8523       05cp21223_4725443_f3_14   3762   8524       hp1e10506orf5   3763   8525       09ap10418orf1   3764   8526       hpy09ap10418orf_0001.aa   3765   8527       hpy02ce30710orf_0001.aa   3766   8528       06ep30223_7304760_c1_113   3767   8529       01ce21104_13789077_c2_71   3768   8530       hpy05cp11911orf_0014.aa   3769   8531       hpy02ae11612orf_0009.aa   3770   8532       02ae11612orf27   3771   8533       02ae11612_16664827_c2_85   3772   8534       02ae11612_19535962_f1_12   3773   8535       05cp11911orf14   3774   8536       06ep11917_22692800_c3_26   3775   8537       hp4e14535orf5   3776   8538       hpy02ae11612orf_0015.aa   3777   8539       hpy02ae11612orf_0014.aa   3778   8540       01ce21104_24484438_c1_64   3779   8541       01ce11104_24803330_f2_3   3780   8542       16ep10117orf1   3781   8543       01ce21104_4554713_c2_80   3782   8544       hpy02ae11612orf_0016.aa   3783   8545       hpy02ae11612orf_0019.aa   3784   8546       hpy02ae11612orf_0020.aa   3785   8547       01ce21104_4804068_c3_100   3786   8548       10664078   3787   8549       02ae11612_5114693_f1_16   3788   8550       05cp11911orf11   3789   8551       02ae11612_6837812_f3_45   3790   8552       05cp11911orf9   3791   8553       hp5p15575_4729717_f1_1   3792   8554       hpyhp5p15575orf_0004.aa   3793   8555       09ap10515_16212692_f2_1   3794   8556       03ge31106orf2   3795   8557       01xe21717orf28   3796   8558       05ep10815_23441262_f3_61   3797   8559       hp6p10509_12618813_c2_16   3798   8560       hpy16ae10508orf_0006.aa   3799   8561       14864452   3800   8562       hp6p10509_25417812_c3_18   3801   8563       16ae10508orf10   3802   8564       hp6p10522_79703_f1_1   3803   8565       19541302   3804   8566       hp6p10590_14742212_f2_13   3805   8567       11gp11422orf2   3806   8568       hpy11gp11422orf_0001.aa   3807   8569       hp6p10590_33336566_f3_21   3808   8570       05ae10218orf3   3809   8571       hpy04ee70114orf_0005.aa   3810   8572       hpy04ee70114orf_0003.aa   3811   8573       hp6p10590_4350342_c3_40   3812   8574       hp6p10590_4688818_c2_33   3813   8575       01gp10401orf2   3814   8576       4787562   3815   8577       hp6p10590_4787562_f1_5   3816   8578       11gp11422orf1   3817   8579       01ep12002_16457267_c2_17   3818   8580       04ep10515orf10   3819   8581       hpy04ep10515orf_0002.aa   3820   8582       hp6p12129_26172077_c1_20   3821   8583       hp6p12129_26172077_c2_23   3822   8584       hpyhp3e10302orf_0018.aa   3823   8585       hp3e10302orf24   3824   8586       hp6p12129_5320151_f3_13   3825   8587       14cp10923orf4   3826   8588       14cp10923_10181291_f2_8   3827   8589       14cp10923_10427207_f2_7   3828   8590       14cp10923orf6   3829   8591       hp1p13922orf2   3830   8592       hp1p13922orf7   3831   8593       hp6p12244_10828331_f3_39   3832   8594       24611590   3833   8595       hp1p13922orf30   3834   8596       34089087   3835   8597       hp1p13922orf22   3836   8598       hp6p12244_14539063_c2_72   3837   8599       hp6p12244_14628127_c1_51   3838   8600       hp6p12244_21688926_c1_63   3839   8601       14cp10923_22554712_f1_5   3840   8602       hp5e15653orf2   3841   8603       14cp10923_24023442_f1_2   3842   8604       14cp10923orf2   3843   8605       hpyhp1p13922orf_0015.aa   3844   8606       hp6p12244_242291_c1_50   3845   8607       03ap20115orf2   3846   8608       03ap20115orf1   3847   8609       hp1p13922orf6   3848   8610       hp6p12244_24415938_f1_9   3849   8611       hpyhp1p13922orf_0009.aa   3850   8612       hp1p13922orf17   3851   8613       hp6p12244_25429505_f3_40   3852   8614       3242952   3853   8615       14cp10923_3242952_f1_3   3854   8616       14cp10923orf3   3855   8617       hp1p13922orf4   3856   8618       hp6p12244_33214692_f2_26   3857   8619       hp1p13922orf3   3858   8620       hp1p13922orf8   3859   8621       16603381   3860   8622       hp6p12244_33782812_f2_25   3861   8623       24492192   3862   8624       14cp10923_4864062_f1_1   3863   8625       14cp10923orf1   3864   8626       06ae11915orf5   3865   8627       hp6p12244_21975662_f1_8   3866   8628       hp6p12244_5282838_f3_39   3867   8629       10580417   3868   8630       hp6p22217_10580417_f3_17   3869   8631       13ee12016orf19   3870   8632       hp6p22217_16459375_c3_31   3871   8633       16459375   3872   8634       13ee12016orf24   3873   8635       hp6p22217_21667317_c1_22   3874   8636       13ee12016orf33   3875   8637       13ae10712_22775033_f1_3   3876   8638       13ae10712orf3   3877   8639       13ae10712_23626592_f1_5   3878   8640       01ae11421orf5   3879   8641       13ae10712_23850312_f2_9   3880   8642       13ae10712orf7   3881   8643       02ge10814orf1   3882   8644       11ae12004_6906717_f3_17   3883   8645       3958537   3884   8646       hp7e10429_10720208_c3_42   3885   8647       07ge20415orf22   3886   8648       hpy07ge20415orf_0015.aa   3887   8649       hp7e10429_14495812_c1_27   3888   8650       hp7e10429_24297188_c3_43   3889   8651       07ge20415orf23   3890   8652       12ap11614_32711508_f3_6   3891   8653       12ap11614orf7   3892   8654       hp7e10429_34172291_c1_26   3893   8655       07ge20415orf21   3894   8656       07ge20415orf37   3895   8657       07ge20415orf36   3896   8658       hp7e10429_41018_c1_32   3897   8659       07ge20415orf29   3898   8660       hpy07ge20415orf_0021.aa   3899   8661       hp7e10429_4859652_c2_35   3900   8662       07ge20415orf18   3901   8663       07ge20415orf13   3902   8664       hp7e10429_5906337_f1_3   3903   8665       hp5p15504orf4   3904   8666       hp5p15504orf1   3905   8667       hpyhp5p15504orf_0003.aa   3906   8668       hp7e10433_32460937_c3_12   3907   8669       14ce61516_17000776_c2_29   3908   8670       07ee11905orf3   3909   8671       11ae80818_22692925_f3_36   3910   8672       hpy06cp11722orf_0010.aa   3911   8673       hpy14ep11905orf_0010.aa   3912   8674       14ce61516_236452_f3_15   3913   8675       11ae80818_25957137_c3_65   3914   8676       11ae80818_39017_f3_35   3915   8677       06cp11722orf13   3916   8678       11ae80818_3954717_c3_66   3917   8679       hpy05ae10808orf_0004.aa   3918   8680       11ae80818_36532292_c2_54   3919   8681       03ap21820orf7   3920   8682       11ae80818_5885942_c1_48   3921   8683       hp7e10434_17070306_f2_12   3922   8684       hp7e10434_24101635_c1_28   3923   8685       06ce10515orf5   3924   8686       hpy06ce10515orf_0009.aa   3925   8687       hp7e10434_31417090_f3_17   3926   8688       01gp11016_24220438_c3_17   3927   8689       01gp11016orf18   3928   8690       hp7e30434_31417090_f1_3   3929   8691       12ae21111orf2   3930   8692       hp6e50324_33386312_c1_10   3931   8693       hp7e10434_3939018_f1_3   3932   8694       06ce10515orf6   3933   8695       hp7e10434_4181638_f1_4   3934   8696       06ce10515orf7   3935   8697       06ce10515orf3   3936   8698       06ce10515orf1   3937   8699       hp7e10434_4414092_f3_15   3938   8700       12ae21111orf1   3939   8701       hpy12ae21111orf_0001.aa   3940   8702       hp6e50324_23485714_c1_9   3941   8703       hp7e30434_4767717_f2_20   3942   8704       hp5p15249orf1   3943   8705       hpyhp5p15249orf_0001.aa   3944   8706       hp7e10192_12150293_f2_4   3945   8707       hp7e10192_12150293_f2_2   3946   8708       hp7p10191_35830427_f1_1   3947   8709       hpy01ce11513orf_0013.aa   3948   8710       01ce11513orf5   3949   8711       02ep20506_4334693_f3_11   3950   8712       hp7p10287_4334693_c3_28   3951   8713       hpy01ce11513orf_0016.aa   3952   8714       01ce11513orf7   3953   8715       hp7p10287_4959717_c1_19   3954   8716       02ce41018_30156593_c1_27   3955   8717       hp3p10349orf13   3956   8718       hp7e10420_34381937_c1_31   3957   8719       hpy07gp10508orf_0001.aa   3958   8720       02ce41018_4884662_c2_31   3959   8721       hp3p10349orf14   3960   8722       07ap20216_2239637_f3_8   3961   8723       05ap21216orf2   3962   8724       11ap20714_26803187_c3_105   3963   8725       hp5e15440orf22   3964   8726       07ap20216_36126715_f3_9   3965   8727       05ap21216orf3   3966   8728       11ap20714_3917638_c3_100   3967   8729       hpyhp5e15440orf_0012.aa   3968   8730       02cp10615_1070377_c3_62   3969   8731       11ze30138_12210325_f1_4   3970   8732       09gp10306orf10   3971   8733       11ze30138_127340_f1_5   3972   8734       09gp10306orf8   3973   8735       02cp10615_23447942_c2_56   3974   8736       09gp10306orf6   3975   8737       hpy02cp11721orf_0007.aa   3976   8738       hpy02cp11721orf_0008.aa   3977   8739       02cp10615_23464686_c3_66   3978   8740       11ze30138_24062812_f3_13   3979   8741       09gp10306orf3   3980   8742       11ze30138_24322792_f2_10   3981   8743       09gp10306orf11   3982   8744       02cp10615_24322792_c1_48   3983   8745       09gp10306orf1   3984   8746       hpy09gp10306orf_0001.aa   3985   8747       hp3p10304orf1   3986   8748       02cp10615_24406693_c3_63   3987   8749       11ze30138_24406693_f1_3   3988   8750       09gp10306orf4   3989   8751       11ze30138_29454382_f3_14   3990   8752       02cp10615_29454382_c1_49   3991   8753       hp1p14013_3006541_f3_10   3992   8754       hp1p14013orf7   3993   8755       01ae22001orf4   3994   8756       01ce10218orf1   3995   8757       hpy01ce10218orf_0001.aa   3996   8758       02cp10615_781266_c1_44   3997   8759       hp8e10080_33204166_f2_27   3998   8760       09gp10306orf9   3999   8761       09gp10306orf5   4000   8762       11ze30138_34250066_f3_11   4001   8763       hpy02cp11721orf_0003.aa   4002   8764       02cp11721orf19   4003   8765       hp7e10100_4490967_c3_20   4004   8766       hp6p10606_4960967_c1_20   4005   8767       hpy13ep12003orf_0004.aa   4006   8768       5265957   4007   8769       hp7e10100_5210967_c1_13   4008   8770       02cp11721orf13   4009   8771       hpy01ee11621orf_0002.aa   4010   8772       hpy01ee11621orf_0001.aa   4011   8773       hp6p10606_5368817_f2_14   4012   8774       hpy09gp10306orf_0007.aa   4013   8775       hpy09gp10306orf_0006.aa   4014   8776       02cp10615_6831900_c1_47   4015   8777       14570443   9613   9725       14645905   9614   9726       29557266   9615   9727       214812   9616   9728       1370202   9617   9729       4821082   9618   9730       34489543   9619   9731       21618785   9620   9732       22667967   9621   9733       C. SECRETED PROTEINS       C.1 Periplasmic proteins       03ae10804orf1   4016   8778       03ae10804_23473912_f1_1   4017   8779       hp1e80523_24415942_f3_27   4018   8780       C.2 Proteins involved in secretion and       adhesion       12ap10324_3906712_f3_5   4019   8781       3906712   4020   8782       12ap10324orf3   4021   8783       07gp31516orf3   4022   8784       12ap10324orf6   4023   8785       12ap10324orf5   4024   8786       12ap10324_4805318_f2_3   4025   8787       12ap10324_4805318_f2_6   4026   8788       4805318   9622   9734       C.3 Chaperones       50253   4027   8789       hp5e15211_50253_f2_13   4028   8790       hp5e15211_50253_f2_16   4029   8791       hp5e15211orf10   4030   8792       C.4 Other secreted proteins       09cp61003_23593955_c1_79   4031   8793       hp6p80503_17032125_f1_1   4032   8794       01ce61016_23593955_c3_140   4033   8795       01ce61016_23609580_c3_139   4034   8796       09cp11003_19532625_c3_17   4035   8797       09cp61003_19532625_c1_78   4036   8798       14ge10705orf14   4037   8799       07cp11213orf1   4038   8800       hpy07cp11213orf_0001.aa   4039   8801       11ee10423orf4   4040   8802       09cp61003_24335762_c3_111   4041   8803       hp3p10156orf6   4042   8804       hp3p10156orf2   4043   8805       hp7p10290_25548812_f3_14   4044   8806       hp6e12267_4876718_f2_23   4045   8807       12ge10305orf5   4046   8808       09ap20802orf13   4047   8809       hp2p10272_22692325_f3_21   4048   8810       02ap11117_23495187_c3_81   4049   8811       09ap20802orf14   4050   8812       hp2p10272_23697200_f3_22   4051   8813       hp2p10272_26829136_f1_1   4052   8814       hp2p10272orf1   4053   8815       29ep10720_289077_f2_12   4054   8816       289077   4055   8817       11ge10309orf9   4056   8818       02gp20706_1203402_c3_58   4057   8819       09ge70821orf2   4058   8820       02gp20706_15781452_c2_51   4059   8821       02ge10116_15781452_c1_87   4060   8822       06ge10115orf17   4061   8823       36335436   4062   8824       02ge10116_36335436_f3_66   4063   8825       hp5e15276orf14   4064   8826       02gp20706_4892558_f3_19   4065   8827       01cp11710orf1   4066   8828       02ge41622_20730462_f1_19   4067   8829       hpy13ee10216orf_0035.aa   4068   8830       02ge11622_21695936_c1_54   4069   8831       13ee10216orf57   4070   8832       23594838   4071   8833       06ee30709_33851038_c3_30   4072   8834       01ae11403orf1   4073   8835       hpy01ae11403orf_0001.aa   4074   8836       05ae30220_14570443_c2_94   4075   8837       05ae20220orf124   4076   8838       01cp20708_10628177_c2_50   4077   8839       22447252   4078   8840       05ep10815_22447252_c3_110   4079   8841       07ge11504orf3   4080   8842       30283516   4081   8843       05ep10815_30283516_c3_109   4082   8844       07ge11504orf2   4083   8845       hp1p10543orf4   4084   8846       hpyhp1p10543orf_0004.aa   4085   8847       hp1e10554orf1   4086   8848       05ep10815_4195292_c1_84   4087   8849       24328910   4088   8850       hp5e15211_24328910_c3_34   4089   8851       hp5e15211_24328910_c3_38   4090   8852       hp5e15211orf21   4091   8853       hp5e15211_819455_c2_24   4092   8854       hp5e15211orf23   4093   8855       11876471   4094   8856       04ep41903_11876461_f1_4   4095   8857       09ae11601orf4   4096   8858       05ae30220_21619067_f3_56   4097   8859       05ae20220orf58   4098   8860       05ae30220_21720017_f2_23   4099   8861       21720017   4100   8862       05ae20220orf24   4101   8863       05ae30220_24410643_c1_79   4102   8864       24410643   4103   8865       05ae20220orf92   4104   8866       hpy05ae20220orf_0031.aa   4105   8867       hpy05ae20220orf_0030.aa   4106   8868       05ae30220_24415693_c3_175   4107   8869       05ae30220_24882812_c3_103   4108   8870       05ae20220orf119   4109   8871       05ae30220_25953163_c3_98   4110   8872       05ae20220orf95   4111   8873       4882318   4112   8874       14ep11115orf2   4113   8875       14ep11115orf1   4114   8876       11ee11408_4882318_f3_24   4115   8877       05ae30220_80257_f3_46   4116   8878       80257   4117   8879       05ae20220orf50   4118   8880       02ce10213orf5   4119   8881       24276587   4120   8882       02ce10213orf20   4121   8883       02ce10213orf11   4122   8884       hp5p15212_24276587_f1_2   4123   8885       hp5p15212_34064750_f2_9   4124   8886       02ce10213orf19   4125   8887       hpy11ce10908orf_0002.aa   4126   8888       11ce10908orf1   4127   8889       hpy11ce10908orf_0001.aa   4128   8890       hp5p15212_6928132_c3_34   4129   8891       12ae11404orf9   4130   8892       12ae11404orf3   4131   8893       12ae11404_22303918_f3_6   4132   8894       22303918   4133   8895       3166040   4134   8896       09ae10512_3166040_c1_40   4135   8897       hp3e11060orf9   4136   8898       32462543   4137   8899       01ae12001_32462543_c2_43   4138   8900       07gp11807orf32   4139   8901       34161500   4140   8902       719606   4141   8903       07gp11807orf54   4142   8904       07gp11807orf42   4143   8905       06ap10609_3952_c3_55   4144   8906       06ap11119_16594193_f1_9   4145   8907       11ge10309orf7   4146   8908       hp6p10723orf7   4147   8909       06ap11119_24406401_f3_23   4148   8910       11ge10309orf25   4149   8911       06ap11119_24406401_f3_23   4150   8912       24406401   4151   8913       06cp11118orf7   4152   8914       06cp11118_212827_c1_17   4153   8915       04ce11617orf4   4154   8916       hpyhp1p11256orf_0002.aa   4155   8917       05ee10816_14649077_f3_18   4156   8918       06ep10615_14649077_f2_30   4157   8919       06ep10615_14649077_f3_52   4158   8920       hp6e10967_23476502_f2_6   4159   8921       hp2p10625orf5   4160   8922       hp6e10967_24882750_f2_7   4161   8923       hp2p10625orf6   4162   8924       04ce11617orf10   4163   8925       05ee10816_259703_f2_7   4164   8926       21687842   4165   8927       06ep11202_21687842_c3_35   4166   8928       hp2e11858orf5   4167   8929       06gp10409_4015687_f2_11   4168   8930       14cp10119orf1   4169   8931       04cp11202_16603425_c2_72   4170   8932       04ge11713orf10   4171   8933       04cp11202_19797128_f1_5   4172   8934       04cp11202_24261588_f2_23   4173   8935       06gp71906_24261588_c2_174   4174   8936       01ae12021orf5   4175   8937       29386577   4176   8938       12gp31106_29386577_f2_24   4177   8939       13ap11517orf7   4178   8940       12gp31106_3024126_f2_25   4179   8941       13ap11517orf15   4180   8942       12gp31106_24411308_f1_13   4181   8943       13ap11517orf10   4182   8944       06gp71906_25401078_c2_155   4183   8945       06gp71906_3024126_c1_128   4184   8946       09cp20502_24001388_c1_31   4185   8947       14gp11820orf1   4186   8948       14ap10815_16603418_c3_26   4187   8949       16603418   4188   8950       hp3e10349orf24   4189   8951       14ap10815_23439055_c2_21   4190   8952       23439055   4191   8953       hp3e10349orf17   4192   8954       07ap11111_234693_c3_14   4193   8955       07ap11111orf13   4194   8956       hpyhp2e10911orf_0017.aa   4195   8957       hpyhp2e10911orf_0016.aa   4196   8958       hp2e10911_10213593_c1_73   4197   8959       hpy01cp11108orf_0002.aa   4198   8960       hpy01cp11108orf_0001.aa   4199   8961       hp2e10911orf24   4200   8962       hp2e10911orf25   4201   8963       hp2e10911_35567005_c2_88   4202   8964       07ap11213_35401528_c1_21   4203   8965       07ee50709_10213593_f3_77   4204   8966       02ae31010_16833312_f2_19   4205   8967       02ce11022orf2   4206   8968       29ge10307orf4   4207   8969       hp5p15641_24304527_c3_35   4208   8970       29ge10307orf3   4209   8971       hp5p15641_25635452_c3_34   4210   8972       hp1p13947orf11   4211   8973       01ce11618orf1   4212   8974       hpy01ce11618orf_0001.aa   4213   8975       02ae31010_34616666_f2_27   4214   8976       01ep11504orf5   4215   8977       hpy01ep11504orf_0005.aa   4216   8978       02ae31010_35270000_f3_33   4217   8979       01ce11618orf3   4218   8980       01ce11618orf13   4219   8981       02ae31010_36132785_f2_29   4220   8982       01cp11108orf6   4221   8983       hpy01cp11108orf_0006.aa   4222   8984       hpy01cp11108orf_0005.aa   4223   8985       hpy01cp11108orf_0004.aa   4224   8986       11cp12006orf17   4225   8987       hp2e10911_960952_c2_86   4226   8988       291700   4227   8989       hp2e10911_13871077_c3_97   4228   8990       hp2e10911_4882027_c2_87   4229   8991       hpy11cp12006orf_0001.aa   4230   8992       07ee50709_960952_f2_47   4231   8993       35336707   4232   8994       02ce10216orf2   4233   8995       02ce10216orf1   4234   8996       09ce10413_35336707_f2_9   4235   8997       09ce10413_414011_f1_3   4236   8998       02ce10216orf6   4237   8999       02ce10216orf7   4238   9000       02ce10216orf4   4239   9001       hpy02ce10216orf_0007.aa   4240   9002       09ce10413_5865665_f1_4   4241   9003       09ce52017_29324062_c1_21   4242   9004       01ep30520orf24   4243   9005       03ge10505orf6   4244   9006       hpy11cp71403orf_0002.aa   4245   9007       09ze10333_1457137_f3_11   4246   9008       06cp30603_10744075_c3_136   4247   9009       hpy04gp11213orf_0010.aa   4248   9010       hp6e20339_1190660_c2_46   4249   9011       04gp11213orf22   4250   9012       06cp30603_21492187_f2_41   4251   9013       hp6e20339_21492187_c1_40   4252   9014       04gp11213orf14   4253   9015       23912707   4254   9016       09cp10713_23912707_c1_27   4255   9017       09cp10713_23912707_c1_26   4256   9018       09cp10713orf25   4257   9019       06cp30603_2772578_c1_46   4258   9020       2843912   4259   9021       05cp20518orf41   4260   9022       06cp30603_2772578_c2_117   4261   9023       hp6e20339_34024187_c1_37   4262   9024       04gp11213orf11   4263   9025       hp6e20339_24317062_c3_57   4264   9026       04gp11213orf5   4265   9027       06cp30603_34024187_f1_20   4266   9028       09cp10713_34024187_f1_31   4267   9029       06cp30603_4689068_c3_79   4268   9030       06cp30603orf15   4269   9031       12ge10321_24308513_f3_20   4270   9032       06gp11920orf11   4271   9033       06gp11920orf7   4272   9034       06gp11920orf6   4273   9035       12ae10622_30273255_f1_13   4274   9036       03ae10804_23485968_c3_47   4275   9037       06ep10306orf5   4276   9038       03ae10804_16187640_c2_36   4277   9039       06ep10306orf15   4278   9040       hp1e80523_23485968_c2_49   4279   9041       01ge11619_22448587_c1_9   4280   9042       hp3e10342_22448587_c2_15   4281   9043       hpy06ep11108orf_0007.aa   4282   9044       06ep11108orf17   4283   9045       hp4e13394_35957200_f1_21   4284   9046       hp4e13394_5088562_f3_54   4285   9047       7116626   4286   9048       hp4e13394_5908553_f1_1   4287   9049       hp4e13394orf2   4288   9050       1416312   4289   9051       03ee11215_1416312_c3_35   4290   9052       03ee11215orf30   4291   9053       hp4e53394_1416312_c3_119   4292   9054       22542803   4293   9055       03ee11215_22542803_f1_7   4294   9056       03ee11215orf10   4295   9057       23631292   4296   9058       05ce10208_23631292_f1_6   4297   9059       14cp11121orf6   4298   9060       05ce10208_26423583_c3_22   4299   9061       26423583   4300   9062       hp1p13939orf9   4301   9063       hpyhp1p13939orf_0004.aa   4302   9064       05ce10208_4707035_c2_17   4303   9065       hpy05ee10411orf_0002.aa   4304   9066       06ep30223_176437_c2_134   4305   9067       2445812   4306   9068       06ep30223_2774062_f1_33   4307   9069       hp3e11024orf22   4308   9070       hp2e10229orf4   4309   9071       06ep30223_5271902_c1_106   4310   9072       02ae11612_1074212_f1_1   4311   9073       02ae11612orf1   4312   9074       01ce11104_10742963_c2_12   4313   9075       16ep10117orf8   4314   9076       10742963   4315   9077       02ae11612_23598175_f1_2   4316   9078       02ae11612orf15   4317   9079       02ae11612_24609431_f1_17   4318   9080       24609431   4319   9081       05cp11911orf12   4320   9082       05cp11911orf35   4321   9083       hpy11ee10118orf_0002.aa   4322   9084       01ce21104_33203250_c3_87   4323   9085       02ae11612_33203250_c1_51   4324   9086       02ae11612_35704718_f2_21   4325   9087       35704718   4326   9088       02ae11612orf4   4327   9089       hp1e10523orf3   4328   9090       01ce11104_36125337_c1_8   4329   9091       hpy14ce10720orf_0006.aa   4330   9092       hp5p15575_26016387_f2_16   4331   9093       hp5p15575_6140713_f2_18   4332   9094       14ce10720orf12   4333   9095       26301059   4334   9096       hp5p23057_16416459_c2_5   4335   9097       09ap10515_36147908_f3_2   4336   9098       03ge31106orf1   4337   9099       hpy02ae11211orf_0008.aa   4338   9100       hpyhp1e13813orf_0001.aa   4339   9101       hp6p12244_4881375_c3_97   4340   9102       23564012   4341   9103       hp6p22217_23564012_f1_5   4342   9104       13ee12016orf8   4343   9105       hp6p22217_272058_f1_2   4344   9106       272058   4345   9107       13ee12016orf5   4346   9108       23958179   4347   9109       13ee12016orf15   4348   9110       hpy13ee12016orf_0014.aa   4349   9111       hp6p22217_2922143_f2_9   4350   9112       02gp20814_24230058_f1_2   4351   9113       24230058   4352   9114       14ee10308orf8   4353   9115       06ce20610_29298537_c2_32   4354   9116       05gp20111orf4   4355   9117       13ae10712_29569208_c2_27   4356   9118       13ae10712orf15   4357   9119       06ce20610_34647187_c2_33   4358   9120       hpy05gp20111orf_0004.aa   4359   9121       06ce20610_3913967_c3_36   4360   9122       05gp20111orf6   4361   9123       hp7e10433_6273452_f1_3   4362   9124       01ee211.18orf1   4363   9125       hp7e10433_36339535_f3_3   4364   9126       hp7e10433_36339535_f3_3   4365   9127       11ae80818_35787667_c2_55   4366   9128       03ap21820orf16   4367   9129       11ae80818_5953343_c1_46   4368   9130       03ap21820orf12   4369   9131       11ae80818_783127_c3_63   4370   9132       03ap21820orf4   4371   9133       14cp11908_783127_c1_72   4372   9134       12ap10324orf4   4373   9135       12ap10324orf8   4374   9136       12ap10324_13178562_f3_6   4375   9137       09cp21607_7224187_c2_12   4376   9138       07gp31516orf9   4377   9139       hpy07gp10508orf_0002.aa   4378   9140       hp7e10420_24391078_f1_3   4379   9141       hp6p10606_15664656_c3_32   4380   9142       hpy13ep12003orf_0008.aa   4381   9143       hpy13ep12003orf_0007.aa   4382   9144       hp6p10606_19546933_c3_31   4383   9145       13ep12003orf21   4384   9146       hp8e10080_19546933_c2_88   4385   9147       23493756   4386   9148       hp6p10606_23493756_c1_21   4387   9149       13ep12003orf20   4388   9150       2035936   9623   9735       2774062   9624   9736       13178562   9625   9737       D. OTHER CELLULAR PROTEINS       24104558   4389   9151       hp7p10290_12693942_f1_5   4390   9152       hp3p10156orf12   4391   9153       24396937   4392   9154       hp6p80503_20964382_f2_11   4393   9155       01ce11016orf14   4394   9156       hpyhp3p10156orf_0005.aa   4395   9157       hpyhp3p10156orf_0004.aa   4396   9158       hp3p10156orf3   4397   9159       hpyhp3p10156orf_0006.aa   4398   9160       hp7p10290_25585941_f3_12   4399   9161       hp6e12267_4095342_f2_17   4400   9162       4095342   4401   9163       12ge20305orf30   4402   9164       12ge10305orf17   4403   9165       12ge10305orf21   4404   9166       hp6e12267_4875342_c2_49   4405   9167       01ge11619_13788141_c2_11   4406   9168       11ge10309orf4   4407   9169       09ap20802orf22   4408   9170       09ap20802orf30   4409   9171       hp2p10272_21671910_c2_38   4410   9172       hpy09ap20802orf_0021.aa   4411   9173       hp2p10272_24406280_c1_26   4412   9174       09ap20802orf21   4413   9175       32704686   4414   9176       09ap20802orf5   4415   9177       hp2p10272_34042518_f1_2   4416   9178       09ap20802orf8   4417   9179       09ap20802orf1   4418   9180       hp2p10272_3906568_f3_23   4419   9181       29ep10720_24220926_f2_8   4420   9182       hp4e12063orf1   4421   9183       11ge10309orf56   4422   9184       12ae10224orf1   4423   9185       11ge10309orf66   4424   9186       29ep10720_24495312_c1_28   4425   9187       06ge10115orf19   4426   9188       02gp20706_23866562_c2_53   4427   9189       14ae10212orf1   4428   9190       hpy14ae10212orf_0001.aa   4429   9191       01cp11710orf33   4430   9192       02gp20706_6644630_c3_62   4431   9193       02gp20706_4083212_c1_43   4432   9194       02ge10116_23866562_c3_146   4433   9195       02ge10116_36617176_f1_7   4434   9196       02ge20116orf19   4435   9197       05ep20322orf4   4436   9198       05ep20322orf10   4437   9199       05ep20322orf2   4438   9200       hp6p12311_4111712_c1_8   4439   9201       02gp20706_4491093_c1_39   4440   9202       4491093   4441   9203       06ge10115orf12   4442   9204       914087   4443   9205       02ge11622_25428152_c1_52   4444   9206       13ee10216orf55   4445   9207       6136430   4446   9208       06ee10709_6136430_c1_11   4447   9209       06ee10709orf17   4448   9210       06ae11405orf8   4449   9211       22687687   4450   9212       06ae11405orf10   4451   9213       05ep10815_2087568_c3_101   4452   9214       hpy07cp10312orf_0007.aa   4453   9215       hp3e10185_42813_f3_4   4454   9216       hp6p30249_42813_c1_6   4455   9217       01ae11010_26437877_c2_52   4456   9218       hpyhp4e14522orf_0003.aa   4457   9219       01ae11010_5891077_c3_56   4458   9220       hpyhp4e14522orf_0004.aa   4459   9221       hp4p33322_5891077_c2_45   4460   9222       05ep10815_9785327_c1_80   4461   9223       01xe21717orf20   4462   9224       5312712   4463   9225       24329712   4464   9226       hp5e15211_25411557_c1_22   4465   9227       hp5e15211orf29   4466   9228       29ep10720_24432762_c3_39   4467   9229       11ge10309orf39   4468   9230       14ce31519orf3   4469   9231       15039062   4470   9232       11ee11408_15039062_c1_35   4471   9233       05gp11901orf20   4472   9234       07ap80601_24219012_f2_6   4473   9235       24219012   4474   9236       07ap80601orf12   4475   9237       hpy05ae20220orf_0028.aa   4476   9238       05ae30220_4548792_f2_27   4477   9239       4548792   4478   9240       05ae20220orf6   4479   9241       5078593   4480   9242       07ap80601_5078593_f1_4   4481   9243       07ap80601orf10   4482   9244       05ae30220_54628_c1_116   4483   9245       hp3p21118_54628_c3_3   4484   9246       hpy05ae20220orf_0024.aa   4485   9247       hpy02ce10213orf_0023.aa   4486   9248       02ce10213orf32   4487   9249       02ce10213orf22   4488   9250       hp5p15212_24219500_c1_22   4489   9251       35417942   4490   9252       12ae11404_24789202_f1_2   4491   9253       12ae11404orf8   4492   9254       03ce21717orf1   4493   9255       06ae11016_30579712_f2_21   4494   9256       01ae12001_6490640_c1_34   4495   9257       01ae12001_3952_c3_52   4496   9258       14726542   4497   9259       06ap11119_14726542_f3_21   4498   9260       hp6p10723orf5   4499   9261       06ap11119_23831562_f2_14   4500   9262       23831562   4501   9263       hp6p10723orf20   4502   9264       32952   4503   9265       hp6e10967_32952_c2_20   4504   9266       hp2p10625orf28   4505   9267       6495137   4506   9268       hp6e10967_657638_f3_9   4507   9269       hp2p10625orf8   4508   9270       02ae21214orf1   4509   9271       hpy02ae21214orf_0001.aa   4510   9272       hp2e11858orf6   4511   9273       06ep11202_26353438_c1_22   4512   9274       4569693   4513   9275       06ep11202_4569693_c2_28   4514   9276       06cp20302orf8   4515   9277       06cp20302orf6   4516   9278       06cp20302orf7   4517   9279       06ep11202_792962_c1_18   4518   9280       06cp20302orf2   4519   9281       06ep11202_792962_c2_26   4520   9282       06gp71906_15115637_f2_59   4521   9283       hp4p11393orf2   4522   9284       hpy06ap10209orf_0002.aa   4523   9285       hpy02cp11822orf_0001.aa   4524   9286       06ap10209orf4   4525   9287       06ap10209orf1   4526   9288       hpy06ap10209orf_0001.aa   4527   9289       04cp11202_23553177_c1_75   4528   9290       04cp11202_23553177_c3_109   4529   9291       hpy13ee11718orf_0003.aa   4530   9292       hpy13ee11718orf_0002.aa   4531   9293       1038312   4532   9294       13ee11718orf2   4533   9295       06gp71906_24348416_f2_61   4534   9296       3991067   4535   9297       04cp11202_24413202_c1_65   4536   9298       04ge11713orf41   4537   9299       5111308   4538   9300       04cp11202_5350012_c2_85   4539   9301       04ge11713orf27   4540   9302       hpyhp1p13868orf_0003.aa   4541   9303       hpyhp1p13868orf_0004.aa   4542   9304       07cp10312orf9   4543   9305       391313   4544   9306       14ee41924_236712_f2_31   4545   9307       11ce10917orf9   4546   9308       4572168   4547   9309       hp5p15861_4572168_f3_12   4548   9310       02cp20821orf8   4549   9311       hp2e10911_15680337_c3_105   4550   9312       hpyhp2e10911orf_0011.aa   4551   9313       hp5p15641orf5   4552   9314       hp5p15612orf3   4553   9315       hp5p15641_21563752_f2_10   4554   9316       hpy01ce11618orf_0017.aa   4555   9317       01ce11618orf9   4556   9318       29ge30321_21673965_f2_7   4557   9319       01ce11618orf11   4558   9320       01ce11618orf27   4559   9321       29ge30321_24336712_f1_5   4560   9322       hp2e10911_24804577_c3_104   4561   9323       hpyhp2e10911orf_0012.aa   4562   9324       05ce10613orf2   4563   9325       05ce10613orf1   4564   9326       hpy05ce10613orf_0002.aa   4565   9327       hp5p15641_30273312_c2_28   4566   9328       hpy11ae10212orf_0001.aa   4567   9329       hp2e10911_32234750_c1_68   4568   9330       hp1e10506orf6   4569   9331       17787558   4570   9332       hp5p15641_3907968_f1_3   4571   9333       hp5p15641orf12   4572   9334       05ap10914orf3   4573   9335       hpy05ap10914orf_0001.aa   4574   9336       hp5p15641_5211687_c2_29   4575   9337       06cp11217_4897077_f1_6   4576   9338       hp1p13852orf4   4577   9339       hp6p10904_2214676_c1_14   4578   9340       hp4p13446orf3   4579   9341       hp6p10904_23704412_f2_5   4580   9342       hp4p13446orf13   4581   9343       hp6p10904_7089062_c1_16   4582   9344       hp4p13446orf5   4583   9345       hp4p13402orf2   4584   9346       hpyhp4p13402orf_0004.aa   4585   9347       1256885   4586   9348       hp4p13402orf1   4587   9349       09cp10224_24317005_c3_53   4588   9350       06cp30603_26070252_c3_140   4589   9351       23573294   4590   9352       06cp30603_33984562_c2_56   4591   9353       05cp20518orf50   4592   9354       hpy04gp11213orf_0021.aa   4593   9355       24414687   4594   9356       hp6e20339_4017188_f1_1   4595   9357       25925   4596   9358       12ae10622orf16   4597   9359       12ae10622_4726568_c2_61   4598   9360       03ae10804_235286_f3_19   4599   9361       09ge11604_4804692_c1_8   4600   9362       hpy05ap11505orf_0005.aa   4601   9363       hp2p10610_21987687_c2_5   4602   9364       01ge11619_23711062_c3_14   4603   9365       11ge10309orf12   4604   9366       01ge11619_24415880_c2_12   4605   9367       11ge10309orf5   4606   9368       01ge11619_24417813_c1_8   4607   9369       11ge10309orf24   4608   9370       hpyhp3p10807orf_0005.aa   4609   9371       hp3p10807_29343768_f1_1   4610   9372       hp3p10807orf4   4611   9373       hp3p10807_29352212_f2_5   4612   9374       hp3p10807orf7   4613   9375       hp3e11188_5082842_f3_12   4614   9376       06ee11611orf1   4615   9377       hp4e13394_26182793_f2_45   4616   9378       hpy06ep11108orf_0004.aa   4617   9379       hpy03ee11215orf_0001.aa   4618   9380       hpyhp3e10975orf_0005.aa   4619   9381       hp4e53394_2082126_c2_102   4620   9382       hp3e11168orf35   4621   9383       05ce10208_5211002_c2_15   4622   9384       14cp11121orf10   4623   9385       01ge10203orf14   4624   9386       01ge10203orf7   4625   9387       01ge10203_35281542_c3_16   4626   9388       hp4p62853_5914693_c3_52   4627   9389       01ge10203_860166_f3_9   4628   9390       01ge10203orf6   4629   9391       hp1e10506orf2   4630   9392       06ce11002orf3   4631   9393       06ce11002orf8   4632   9394       06ce11002_194415_f2_4   4633   9395       06ep30223_25402187_c1_112   4634   9396       3930468   4635   9397       hpy02ce11220orf_0003.aa   4636   9398       02ce11220orf2   4637   9399       06ep30223_3930468_c1_110   4638   9400       06ep30223_4876077_c3_149   4639   9401       hp3e11024orf34   4640   9402       hp6e10491_12712706_f3_12   4641   9403       hpyhp5e15084orf_0002.aa   4642   9404       30703183   4643   9405       hp6p10509_12600691_c3_22   4644   9406       16ae10508orf14   4645   9407       14642217   4646   9408       16ae10508orf13   4647   9409       hp6p10509_14642217_c2_17   4648   9410       hp6p10509_14642217_c3_25   4649   9411       hpy04ee70114orf_0001.aa   4650   9412       04ee70114orf10   4651   9413       hp6p10590_23440913_c2_31   4652   9414       hp6p10847_26194552_f2_1   4653   9415       14094816   4654   9416       12ap10605_14094816_c1_5   4655   9417       29gp10119orf6   4656   9418       hpyhp3e10302orf_0016.aa   4657   9419       hp3e10302orf26   4658   9420       hp6p12129_16603417_f3_14   4659   9421       hp6p12129_12542880_c3_29   4660   9422       hpy04ep10515orf_0001.aa   4661   9423       hp6p12129_17067265_c3_29   4662   9424       hp6p12129_214055_f1_2   4663   9425       hp6p12129_214055_f3_17   4664   9426       hpy29gp10119orf_0003.aa   4665   9427       12ap10605_30603402_c1_4   4666   9428       30603402   4667   9429       29gp10119orf5   4668   9430       hp6p12244_33492712_c3_88   4669   9431       hp6p12244_32458287_c3_90   4670   9432       hp5p15653orf3   4671   9433       hp5p15653orf1   4672   9434       hp5p15653orf2   4673   9435       hpyhp5p15653orf_0001.aa   4674   9436       hp4p12005orf2   4675   9437       hp6p12244_3948467_c1_52   4676   9438       hp6p12244_3948467_c3_88   4677   9439       hp6p22217_23470967_f1_4   4678   9440       13ee12016orf7   4679   9441       01ae11421orf6   4680   9442       01ae11421orf1   4681   9443       03ge10505orf1   4682   9444       hpy03ge10505orf_0001.aa   4683   9445       13ae10712_24415957_f2_13   4684   9446       12ap11614_26054702_c1_8   4685   9447       hp7e10429_26054702_f3_23   4686   9448       12ap11614orf8   4687   9449       26054702   4688   9450       07ge20415orf6   4689   9451       24634750   4690   9452       14ce21516_24634750_f2_3   4691   9453       14ce21516orf3   4692   9454       hp7e10192_4412568_f2_5   4693   9455       02ce10114orf3   4694   9456       16440842   4695   9457       02ce10114orf1   4696   9458       hp7e10192_5917593_f1_2   4697   9459       02ep20506_24611325_f2_6   4698   9460       01ce11513orf17   4699   9461       hp7p10287_24611325_c2_24   4700   9462       07gp10508orf4   4701   9463       hpy07gp10508orf_0003.aa   4702   9464       hp3p10349orf16   4703   9465       02ce41018_25509687_f3_18   4704   9466       hp5e15440orf19   4705   9467       489057   9626   9738       5879160   9627   9739       2738378   9628   9740       24495312   9629   9741       16839562   9630   9742       21563752   9631   9743       194415   9632   9744       24300682   9633   9745       12897656   9634   9746       36594167   9635   9747       4492217   9636   9748       E. MEMBRANE ASSOCIATED PROTEINS       09cp61003_1410927_f1_14   4706   9468       09cp61003_5079842_c3_112   4707   9469       01ce61016_4960283_c2_142   4708   9470       09cp61003_78267_c1_61   4709   9471       02ge10116_34584465_c3_160   4710   9472       02ge41622_14875000_c1_53   4711   9473       hpy13ee10216orf_0032.aa   4712   9474       07gp10513_167167_f3_2   4713   9475       27ze10351orf8   4714   9476       hpy27ze10351orf_0014.aa   4715   9477       05ae30220_19728437_c1_102   4716   9478       05ae30220_24244512_c3_172   4717   9479       hpy05ae20220orf_0037.aa   4718   9480       05ae30220_26758552_c2_157   4719   9481       hpy05ae20220orf_0005.aa   4720   9482       06ap10609_2738188_f3_30   4721   9483       07gp11807orf24   4722   9484       hpy07gp11807orf_0014.aa   4723   9485       hpy07gp11807orf_0015.aa   4724   9486       06ap10609_31359837_c3_54   4725   9487       hpy07gp11807orf_0011.aa   4726   9488       hpy07gp11807orf_0012.aa   4727   9489       06ap10609_99192_c2_45   4728   9490       hpyhp6p10723orf_0004.aa   4729   9491       hpyhp6p10723orf_0005.aa   4730   9492       06ap11119_16495313_c1_36   4731   9493       06ep10615_25665908_f1_10   4732   9494       06gp71906_24417581_f3_93   4733   9495       hpy04ge11713orf_0012.aa   4734   9496       hpy11ep12011orf_0002.aa   4735   9497       06gp71906_32219827_c1_125   4736   9498       hpy12gp11106orf_0009.aa   4737   9499       06gp71906_34157561_f3_115   4738   9590       09cp10707_23541676_f2_1   4739   9501       07ae11008_4472192_f2_19   4740   9502       14gp11820orf28   4741   9503       07ap61111_23540930_c2_82   4742   9504       hpyhp1p13868orf_0005.aa   4743   9505       07ap61111_26678562_f1_14   4744   9506       hp3e10349orf1   4745   9507       07cp10312_5880188_f1_1   4746   9508       02ae31010_24040901_c3_103   4747   9509       02ae31010_992092_f3_39   4748   9510       hpyhp1p13947orf_0012.aa   4749   9511       09cp10224_3209752_c1_40   4750   9512       hpy01ce10516orf_0004.aa   4751   9513       06cp30603_3985338_c3_126   4752   9514       13ae10610_23634567_f3_24   4753   9515       hpy12ee10904orf_0002.aa   4754   9516       hpy05gp11901orf_0008.aa   4755   9517       hpy05gp11901orf_0007.aa   4756   9518       11ee11408_23447952_c2_41   4757   9519       hp3e11188_4882056_c3_34   4758   9520       hpyhp3p10807orf_0004.aa   4759   9521       hp3e11188_4882056_c1_29   4760   9522       11ap20714_4900017_c2_81   4761   9523       05ap21216orf23   4762   9524                  
 
     [0217] [In Table 1, “nt” represents nucleotide Seq. ID number and “aa” represents amino acid Seq. ID number] 
     [0218] Definitions  
     [0219] The terms “purified polypeptide” and “isolated polypeptide” and “a substantially pure preparation of a polypeptide” are used interchangeably herein and, as used herein, mean a polypeptide that has been substantially, and preferably completely, separated from other proteins, lipids, and nucleic acids with which it naturally occurs. Preferably, the polypeptide is also separated from substances, e.g., antibodies or gel matrix, e.g., polyacrylamide, which are used to purify it. Preferably, the polypeptide constitutes at least 10, 20, 50, 70, 80 or 95% dry weight of the purified preparation. Preferably, the preparation contains: sufficient polypeptide to allow protein sequencing; at least 1, 10, or 100 μg of the polypeptide; at least 1, 10, or 100 mg of the polypeptide. Furthermore, the terms “purified polypeptide” and “isolated polypeptide” and “a substantially pure preparation of a polypeptide,” as used herein, refer to both a polypeptide obtained from nature or produced by recombinant DNA techniques as described herein.  
     [0220] For example, an “isolated” or “purified” protein or biologically active portion thereof is substantially free of cellular material or other contaminating proteins from the cell or tissue source from which the  H. pylori  protein is derived, or substantially free from chemical precursors or other chemicals when chemically synthesized. The language “substantially free of cellular material” includes preparations of  H. pylori  protein in which the protein is separated from cellular components of the cells from which it is isolated or recombinantly produced. In one embodiment, the language “substantially free of cellular material” includes preparations of  H. pylori  protein having less than about 30% (by dry weight) of non- H. pylori  protein (also referred to herein as a “contaminating protein”), more preferably less than about 20% of non- H. pylori  protein, still more preferably less than about 10% of non- H. pylori  protein, and most preferably less than about 5% non- H. pylori  protein. When the  H. pylori  protein or biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, i.e., culture medium represents less than about 20%, more preferably less than about 10%, and most preferably less than about 5% of the volume of the protein preparation.  
     [0221] The language “substantially free of chemical precursors or other chemicals” includes preparations of  H. pylori  protein in which the protein is separated from chemical precusors or other chemicals which are involved in the synthesis of the protein. In one embodiment, the language “substantially free of chemical precursors or other chemicals” includes preparations of  H. pylori  protein having less than about 30% (by dry weight) of chemical precursors or non- H. pylori  chemicals, more preferably less than about 20% chemical precursors or non- H. pylori  chemicals, still more preferably less than about 10% chemical precursors or non- H. pylori  chemicals, and most preferably less than about 5% chemical precursors or non- H. pylori  chemicals.  
     [0222] A purified preparation of cells refers to, in the case of plant or animal cells, an in vitro preparation of cells and not an entire intact plant or animal. In the case of cultured cells or microbial cells, it consists of a preparation of at least 10% and more preferably 50% of the subject cells.  
     [0223] A purified or isolated or a substantially pure nucleic acid, e.g., a substantially pure DNA, (are terms used interchangeably herein) is a nucleic acid which is one or both of the following: not immediately contiguous with both of the coding sequences with which it is immediately contiguous (i.e., one at the 5′ end and one at the 3′ end) in the naturally-occurring genome of the organism from which the nucleic acid is derived; or which is substantially free of a nucleic acid with which it occurs in the organism from which the nucleic acid is derived. The term includes, for example, a recombinant DNA which is incorporated into a vector, e.g., into an autonomously replicating plasmid or virus, or into the genomic DNA of a prokaryote or eukaryote, or which exists as a separate molecule (e.g., a cDNA or a genomic DNA fragment produced by PCR or restriction endonuclease treatment) independent of other DNA sequences. Substantially pure DNA also includes a recombinant DNA which is part of a hybrid gene encoding additional  H. pylori  DNA sequence.  
     [0224] A “contig” as used herein is a nucleic acid representing a continuous stretch of genomic sequence of an organism.  
     [0225] An “open reading frame”, also referred to herein as ORF, is a region of nucleic acid which encodes a polypeptide. This region may represent a portion of a coding sequence or a total sequence and can be determined from a stop to stop codon or from a start to stop codon.  
     [0226] As used herein, a “coding sequence” is a nucleic acid which is transcribed into messenger RNA and/or translated into a polypeptide when placed under the control of appropriate regulatory sequences. The boundaries of the coding sequence are determined by a translation start codon at the five prime terminus and a translation stop code at the three prime terminus. A coding sequence can include but is not limited to messenger RNA, synthetic DNA, and recombinant nucleic acid sequences.  
     [0227] A “complement” of a nucleic acid as used herein referes to an anti-parallel or antisense sequence that participates in Watson-Crick base-pairing with the original sequence.  
     [0228] A “gene product” is a protein or structural RNA which is specifically encoded by a gene.  
     [0229] As used herein, the term “probe” refers to a nucleic acid, peptide or other chemical entity which specifically binds to a molecule of interest. Probes are often associated with or capable of associating with a label. A label is a chemical moiety capable of detection. Typical labels comprise dyes, radioisotopes, luminescent and chemiluminescent moieties, fluorophores, enzymes, precipitating agents, amplification sequences, and the like. Similarly, a nucleic acid, peptide or other chemical entity which specifically binds to a molecule of interest and immobilizes such molecule is referred herein as a “capture ligand”. Capture ligands are typically associated with or capable of associating with a support such as nitro-cellulose, glass, nylon membranes, beads, particles and the like. The specificity of hybridization is dependent on conditions such as the base pair composition of the nucleotides, and the temperature and salt concentration of the reaction. These conditions are readily discernable to one of ordinary skill in the art using routine experimentation.  
     [0230] Homologous refers to the sequence similarity or sequence identity between two polypeptides or between two nucleic acid molecules. When a position in both of the two compared sequences is occupied by the same base or amino acid monomer subunit, e.g., if a position in each of two DNA molecules is occupied by adenine, then the molecules are homologous at that position. The percent of homology between two sequences is a function of the number of matching or homologous positions shared by the two sequences divided by the number of positions compared×100. For example, if 6 of 10 of the positions in two sequences are matched or homologous then the two sequences are 60% homologous. By way of example, the DNA sequences ATTGCC and TATGGC share 50% homology. Generally, a comparison is made when two sequences are aligned to give maximum homology.  
     [0231] Nucleic acids are hybridizable to each other when at least one strand of a nucleic acid can anneal to the other nucleic acid under defined stringency conditions. Stringency of hybridization is determined by: (a) the temperature at which hybridization and/or washing is performed; and (b) the ionic strength and polarity of the hybridization and washing solutions. Hybridization requires that the two nucleic acids contain complementary sequences; depending on the stringency of hybridization, however, mismatches may be tolerated. Typically, hybridization of two sequences at high stingency (such as, for example, in a solution of 0.5×SSC, at 65° C.) requires that the sequences be essentially completely homologous. Conditions of intermediate stringency (such as, for example, 2×SSC at 65° C.) and low stringency (such as, for example 2×SSC at 55° C.), require correspondingly less overall complementarity between the hybridizing sequences. (1×SSC is 0.15 M NaCl, 0.015 M Na citrate). A preferred, non-limiting example of stringent hybridization conditions are hybridization in 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 50-65° C.  
     [0232] The terms peptides, proteins, and polypeptides are used interchangeably herein.  
     [0233] As used herein, the term “surface protein” refers to all surface accessible proteins, e.g. inner and outer membrane proteins, proteins adhering to the cell wall, and secreted proteins.  
     [0234] A polypeptide has  H. pylori  biological activity if it has one, two and preferably more of the following properties: (1) if when expressed in the course of an  H. pylori  infection, it can promote, or mediate the attachment of  H. pylori  to a cell; (2) it has an enzymatic activity, structural or regulatory function characteristic of an  H. pylori  protein; (3) the gene which encodes it can rescue a lethal mutation in an  H. pylori  gene; (4) or it is immunogenic in a subject. A polypeptide has biological activity if it is an antagonist, agonist, or super-agonist of a polypeptide having one of the above-listed properties.  
     [0235] A biologically active fragment or analog is one having an in vivo or in vitro activity which is characteristic of the  H. pylori  polypeptides of the invention contained in the Sequence Listing, or of other naturally occurring  H. pylori  polypeptides, e.g., one or more of the biological activities described herein. Especially preferred are fragments which exist in vivo, e.g., fragments which arise from post transcriptional processing or which arise from translation of alternatively spliced RNA&#39;s. Fragments include those expressed in native or endogenous cells as well as those made in expression systems, e.g., in CHO cells. Because peptides such as  H. pylori  polypeptides often exhibit a range of physiological properties and because such properties may be attributable to different portions of the molecule, a useful  H. pylori  fragment or  H. pylori  analog is one which exhibits a biological activity in any biological assay for  H. pylori  activity. Most preferably the fragment or analog possesses 10%, preferably 40%, more preferably 60%, 70%, 80% or 90% or greater of the activity of  H. pylori , in any in vivo or in vitro assay.  
     [0236] Analogs can differ from naturally occurring  H. pylori  polypeptides in amino acid sequence or in ways that do not involve sequence, or both. Non-sequence modifications include changes in acetylation, methylation, phosphorylation, carboxylation, or glycosylation. Preferred analogs include  H. pylori  polypeptides (or biologically active fragments thereof) whose sequences differ from the wild-type sequence by one or more conservative amino acid substitutions or by one or more non-conservative amino acid substitutions, deletions, or insertions which do not substantially diminish the biological activity of the  H. pylori  polypeptide. Conservative substitutions typically include the substitution of one amino acid for another with similar characteristics, e.g., substitutions within the following groups: valine, glycine; glycine, alanine; valine, isoleucine, leucine; aspartic acid, glutamic acid; asparagine, glutamine; serine, threonine; lysine, arginine; and phenylalanine, tyrosine. Other conservative substitutions can be made in view of the table below.  
               TABLE 2                          CONSERVATIVE AMINO ACID REPLACEMENTS                         For Amino Acid   Code   Replace with any of               Alanine   A   D-Ala, Gly, beta-Ala, L-Cys, D-Cys       Arginine   R   D-Arg, Lys, D-Lys, homo-Arg,               D-homo-Arg, Met, Ile,               D-Met, D-Ile, Orn, D-Orn       Asparagine   N   D-Asn, Asp, D-Asp, Glu, D-Glu, Gln, D-Gln       Aspartic Acid   D   D-Asp, D-Asn, Asn, Glu, D-Glu, Gln, D-Gln       Cysteine   C   D-Cys, S-Me-Cys, Met, D-Met, Thr, D-Thr       Glutamine   Q   D-Gln, Asn, D-Asn, Glu, D-Glu, Asp, D-Asp       Glutamic Acid   E   D-Glu, D-Asp, Asp, Asn, D-Asn, Gln, D-Gln       Glycine   G   Ala, D-Ala, Pro, D-Pro, β-Ala, Acp       Isoleucine   I   D-Ile, Val, D-Val, Leu, D-Leu, Met, D-Met       Leucine   L   D-Leu, Val, D-Val, Leu, D-Leu, Met, D-Met       Lysine   K   D-Lys, Arg, D-Arg, homo-Arg,               D-homo-Arg, Met, D-               Met, Ile, D-Ile, Orn, D-Orn       Methionine   M   D-Met, S-Me-Cys, Ile, D-Ile,               Leu, D-Leu, Val, D-Val       Phenylalanine   F   D-Phe, Tyr, D-Thr, L-Dopa,               His, D-His, Trp, D-Trp,               Trans-3,4, or 5-phenylproline, cis-3,4,               or 5-phenylproline       Proline   P   D-Pro, L-I-thioazolidine-4-carboxylic               acid, D-or L-1-               oxazolidine-4-carboxylic acid       Serine   S   D-Ser, Thr, D-Thr, allo-Thr,               Met, D-Met, Met(O),               D-Met(O), L-Cys, D-Cys       Threonine   T   D-Thr, Ser, D-Ser, allo-Thr,               Met, D-Met, Met(O),               D-Met(O), Val, D-Val       Tyrosine   Y   D-Tyr, Phe, D-Phe, L-Dopa, His, D-His       Valine   V   D-Val, Leu, D-Leu, Ile, D-Ile, Met, D-Met                  
 
     [0237] Other analogs within the invention are those with modifications which increase peptide stability; such analogs may contain, for example, one or more non-peptide bonds (which replace the peptide bonds) in the peptide sequence. Also included are: analogs that include residues other than naturally occurring L-amino acids, e.g., D-amino acids or non-naturally occurring or synthetic amino acids, e.g., β or γ amino acids; and cyclic analogs.  
     [0238] As used herein, the term “fragment”, as applied to an  H. pylori  analog, will ordinarily be at least about 20 residues, more typically at least about 40 residues, preferably at least about 60 residues in length. Fragments of  H. pylori  polypeptides can be generated by methods known to those skilled in the art. The ability of a candidate fragment to exhibit a biological activity of  H. pylori  polypeptide can be assessed by methods known to those skilled in the art as described herein. Also included are  H. pylori  polypeptides containing residues that are not required for biological activity of the peptide or that result from alternative mRNA splicing or alternative protein processing events.  
     [0239] An “immunogenic component” as used herein is a moiety, such as an  H. pylori  polypeptide, analog or fragment thereof, that is capable of eliciting a humoral and/or cellular immune response in a host animal alone or in combination with an adjuvant.  
     [0240] An “antigenic component” as used herein is a moiety, such as an  H. pylori  polypeptide, analog or fragment thereof, that is capable of binding to a specific antibody with sufficiently high affinity to form a detectable antigen-antibody complex.  
     [0241] As used herein, the term “transgene” means a nucleic acid (encoding, e.g., one or more polypeptides), which is partly or entirely heterologous, i.e., foreign, to the transgenic animal or cell into which it is introduced, or, is homologous to an endogenous gene of the transgenic animal or cell into which it is introduced, but which is designed to be inserted, or is inserted, into the cell&#39;s genome in such a way as to alter the genome of the cell into which it is inserted (e.g., it is inserted at a location which differs from that of the natural gene or its insertion results in a knockout). A transgene can include one or more transcriptional regulatory sequences and any other nucleic acid, such as introns, that may be necessary for optimal expression of the selected nucleic acid, all operably linked to the selected nucleic acid, and may include an enhancer sequence.  
     [0242] As used herein, the term “transgenic cell” refers to a cell containing a transgene.  
     [0243] As used herein, a “transgenic animal” is any animal in which one or more, and preferably essentially all, of the cells of the animal includes a transgene. The transgene can be introduced into the cell, directly or indirectly by introduction into a precursor of the cell, by way of deliberate genetic manipulation, such as by process of transformation of competent cells or by microinjection or by infection with a recombinant virus. This molecule may be integrated within a chromosome, or it may be extrachromosomally replicating DNA.  
     [0244] The term “antibody” as used herein is intended to include fragments thereof which are specifically reactive with  H. pylori  polypeptides.  
     [0245] As used herein, the term “cell-specific promoter” means a DNA sequence that serves as a promoter, i.e., regulates expression of a selected DNA sequence operably linked to the promoter, and which effects expression of the selected DNA sequence in specific cells of a tissue. The term also covers so-called “leaky” promoters, which regulate expression of a selected DNA primarily in one tissue, but cause expression in other tissues as well.  
     [0246] Misexpression, as used herein, refers to a non-wild type pattern of gene expression. It includes: expression at non-wild type levels, i.e., over or under expression; a pattern of expression that differs from wild type in terms of the time or stage at which the gene is expressed, e.g., increased or decreased expression (as compared with wild type) at a predetermined developmental period or stage; a pattern of expression that differs from wild type in terms of decreased expression (as compared with wild type) in a predetermined cell type or tissue type; a pattern of expression that differs from wild type in terms of the splicing size, amino acid sequence, post-transitional modification, or biological activity of the expressed polypeptide; a pattern of expression that differs from wild type in terms of the effect of an environmental stimulus or extracellular stimulus on expression of the gene, e.g., a pattern of increased or decreased expression (as compared with wild type) in the presence of an increase or decrease in the strength of the stimulus.  
     [0247] As used herein, “host cells” and other such terms denoting microorganisms or higher eukaryotic cell lines cultured as unicellular entities refers to cells which can become or have been used as recipients for a recombinant vector or other transfer DNA, and include the progeny of the original cell which has been transfected. It is understood by individuals skilled in the art that the progeny of a single parental cell may not necessarily be completely identical in genomic or total DNA compliment to the original parent, due to accident or deliberate mutation.  
     [0248] As used herein, the term “control sequence” refers to a nucleic acid having a base sequence which is recognized by the host organism to effect the expression of encoded sequences to which they are ligated. The nature of such control sequences differs depending upon the host organism; in prokaryotes, such control sequences generally include a promoter, ribosomal binding site, terminators, and in some cases operators; in eukaryotes, generally such control sequences include promoters, terminators and in some instances, enhancers. The term control sequence is intended to include at a minimum, all components whose presence is necessary for expression, and may also include additional components whose presence is advantageous, for example, leader sequences.  
     [0249] As used herein, the term “operably linked” refers to sequences joined or ligated to function in their intended manner. For example, a control sequence is operably linked to coding sequence by ligation in such a way that expression of the coding sequence is achieved under conditions compatible with the control sequence and host cell.  
     [0250] The metabolism of a substance, as used herein, means any aspect of the, expression, function, action, or regulation of the substance. The metabolism of a substance includes modifications, e.g., covalent or non-covalent modifications of the substance. The metabolism of a substance includes modifications, e.g., covalent or non-covalent modification, the substance induces in other substances. The metabolism of a substance also includes changes in the distribution of the substance. The metabolism of a substance includes changes the substance induces in the distribution of other substances.  
     [0251] A “sample” as used herein refers to a biological sample, such as, for example, tissue or fluid isloated from an individual (including without limitation plasma, serum, cerebrospinal fluid, lymph, tears, saliva and tissue sections) or from in vitro cell culture constituents, as well as samples from the environment.  
     [0252] The practice of the invention will employ, unless otherwise indicated, conventional techniques of chemistry, molecular biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See e.g., Sambrook, Fritsch, and Maniatis,  Molecular Cloning; Laboratory Manual  2nd ed. (1989);  DNA Cloning , Volumes I and II (D. N Glover ed. 1985);  Oligonucleotide Synthesis (M. J. Gait ed,  1984);  Nucleic Acid Hybridization  (B. D. Hames &amp; S. J. Higgins eds. 1984); the series,  Methods in Enzymoloqy  (Academic Press, Inc.), particularly Vol. 154 and Vol. 155 (Wu and Grossman, eds.) and  PCR-A Practical Approach  (McPherson, Quirke, and Taylor, eds., 1991).  
     [0253] I. Isolation of Nucleic Acids of  H. pylori  and Uses Therefor  
     [0254] H. pylori  Genomic Sequence  
     [0255] This invention provides nucleotide sequences of the genome of  H. pylori  which thus comprises a DNA sequence library of  H. pylori  genomic DNA. The detailed description that follows provides nucleotide sequences of  H. pylori , and also describes how the sequences were obtained and how ORFs and protein-coding sequences were identified. Also described are methods of using the disclosed  H. pylori  sequences in methods including diagnostic and therapeutic applications. Furthermore, the library can be used as a database for identification and comparison of medically important sequences in this and other strains of  H. pylori.    
     [0256] To determine the genomic sequence of  H. pylori , DNA was isolated from a strain of  H. pylori  (ATCC # 55679; deposited by Genome Therapeutics Corporation, 100 Beaver Street, Waltham, Mass. 02154) and mechanically sheared by nebulization to a median size of 2 kb. Following size fractionation by gel electrophoresis, the fragments were blunt-ended, ligated to adapter oligonucleotides, and cloned into each of 20 different pMPX vectors (Rice et al., abstracts of Meeting of Genome Mapping and Sequencing, Cold Spring Harbor, N.Y., 5/11-5/15, 1994, p. 225) to construct a series of “shotgun” subclone libraries.  
     [0257] DNA sequencing was achieved using multiplex sequencing procedures essentially as disclosed in Church et al., 1988,  Science  240:185; U.S. Pat. Nos. 4,942,124 and 5,149,625). DNA was extracted from pooled cultures and subjected to chemical or enzymatic sequencing. Sequencing reactions were resolved by electrophoresis, and the products were transferred and covalently bound to nylon membranes. Finally, the membranes were sequentially hybridized with a series of labelled oligonucleotides complimentary to “tag” sequences present in the different shotgun cloning vectors. In this manner, a large number of sequences could be obtained from a single set of sequencing reactions. The cloning and sequencing procedures are described in more detail in the Exemplification.  
     [0258] Individual sequence reads obtained in this manner were assembled using the FALCON™ program (Church et al., 1994,  Automated DNA Sequencing and Analysis , J. C. Venter, ed., Academic Press) and PHRAP (P. Green, Abstracts of DOE Human Genome Program Contractor-Grantee Workshop V, January 1996, p.157). The average contig length was about 3-4 kb.  
     [0259] A variety of approaches are used to order the contigs so as to obtain a continuous sequence representing the entire  H. pylori  genome. Synthetic oligonucleotides are designed that are complementary to sequences at the end of each contig. These oligonucleotides may be hybridized to libaries of  H. pylori  genomic DNA in, for example, lambda phage vectors or plasmid vectors to identify clones that contain sequences corresponding to the junctional regions between individual contigs. Such clones are then used to isolate template DNA and the same oligonucleotides are used as primers in polymerase chain reaction (PCR) to amplify junctional fragments, the nucleotide sequence of which is then determined.  
     [0260] The  H. pylori  sequences were analyzed for the presence of open reading frames (ORFs) comprising at least 180 nucleotides. As a result of the analysis of ORFs based on stop-to-stop codon reads, it should be understood that these ORFs may not correspond to the ORF of a naturally-occurring  H. pylori  polypeptide. These ORFs may contain start codons which indicate the initiation of protein synthesis of a naturally-occurring  H. pylori  polypeptide. Such start codons within the ORFs provided herein can be identified by those of ordinary skill in the relevant art, and the resulting ORF and the encoded  H. pylori  polypeptide is within the scope of this invention. For example, within the ORFs a codon such as AUG or GUG (encoding methionine or valine) which is part of the initiation signal for protein synthesis can be identified and the ORF modified to correspond to a naturally-occurring  H. pylori  polypeptide. The predicted coding regions were defined by evaluating the coding potential of such sequences with the program GENEMARK™ (Borodovsky and Mclninch, 1993,  Comp. Chem.  17:123).  
     [0261] Other  H. pylori  Nucleic Acids  
     [0262] The nucleic acids of this invention may be obtained directly from the DNA of the above referenced  H. pylori  strain by using the polymerase chain reaction (PCR). See “ PCR, A Practical Approach ” (McPherson, Quirke, and Taylor, eds., IRL Press, Oxford, UK, 1991) for details about the PCR. High fidelity PCR can be used to ensure a faithful DNA copy prior to expression. In addition, the authenticity of amplified products can be checked by conventional sequencing methods. Clones carrying the desired sequences described in this invention may also be obtained by screening the libraries by means of the PCR or by hybridization of synthetic oligonucleotide probes to filter lifts of the library colonies or plaques as known in the art (see, e.g., Sambrook et al.,  Molecular Cloning, A Laboratory Manual  2nd edition, 1989, Cold Spring Harbor Press, NY).  
     [0263] It is also possible to obtain nucleic acids encoding  H. pylori  polypeptides from a cDNA library in accordance with protocols herein described. A cDNA encoding an  H. pylori  polypeptide can be obtained by isolating total mRNA from an appropriate strain. Double stranded cDNAs can then be prepared from the total mRNA. Subsequently, the cDNAs can be inserted into a suitable plasmid or viral (e.g., bacteriophage) vector using any one of a number of known techniques. Genes encoding  H. pylori  polypeptides can also be cloned using established polymerase chain reaction techniques in accordance with the nucleotide sequence information provided by the invention. The nucleic acids of the invention can be DNA or RNA. Preferred nucleic acids of the invention are contained in the Sequence Listing.  
     [0264] The nucleic acids of the invention can also be chemically synthesized using standard techniques. Various methods of chemically synthesizing polydeoxynucleotides are known, including solid-phase synthesis which, like peptide synthesis, has been fully automated in commercially available DNA synthesizers (See e.g., Itakura et al. U.S. Pat. No. 4,598,049; Caruthers et al. U.S. Pat. No. 4,458,066; and Itakura U.S. Pat. Nos. 4,401,796 and 4,373,071, incorporated by reference herein).  
     [0265] Nucleic acids isolated or synthesized in accordance with features of the present invention are useful, by way of example, without limitation, as probes, primers, capture ligands, antisense genes and for developing expression systems for the synthesis of proteins and peptides corresponding to such sequences. As probes, primers, capture ligands and antisense agents, the nucleic acid normally consists of all or part (approximately twenty or more nucleotides for specificity as well as the ability to form stable hybridization products) of the nucleic acids of the invention contained in the Sequence Listing. These uses are described in further detail below.  
     [0266] Probes  
     [0267] A nucleic acid isolated or synthesized in accordance with the sequence of the invention contained in the Sequence Listing can be used as a probe to specifically detect  H. pylori . With the sequence information set forth in the present application, sequences of twenty or more nucleotides are identified which provide the desired inclusivity and exclusivity with respect to  H. pylori , and extraneous nucleic acids likely to be encountered during hybridization conditions. More preferably, the sequence will comprise at least twenty to thirty nucleotides to convey stability to the hybridization product formed between the probe and the intended target molecules.  
     [0268] Sequences larger than 1000 nucleotides in length are difficult to synthesize but can be generated by recombinant DNA techniques. Individuals skilled in the art will readily recognize that the nucleic acids, for use as probes, can be provided with a label to facilitate detection of a hybridization product.  
     [0269] Nucleic acid isolated and synthesized in accordance with the sequence of the invention contained in the Sequence Listing can also be useful as probes to detect homologous regions (especially homologous genes) of other Helicobacter species using appropriate stringency hybridization conditions as described herein.  
     [0270] Capture Ligand  
     [0271] For use as a capture ligand, the nucleic acid selected in the manner described above with respect to probes, can be readily associated with a support. The manner in which nucleic acid is associated with supports is well known. Nucleic acid having twenty or more nucleotides in a sequence of the invention contained in the Sequence Listing have utility to separate  H. pylori  nucleic acid from the nucleic acid of each other and other organisms. Nucleic acid having twenty or more nucleotides in a sequence of the invention contained in the Sequence Listing can also have utility to separate other Helicobacter species from each other and from other organisms. Preferably, the sequence will comprise at least twenty nucleotides to convey stability to the hybridization product formed between the probe and the intended target molecules. Sequences larger than 1000 nucleotides in length are difficult to synthesize but can be generated by recombinant DNA techniques.  
     [0272] Primers  
     [0273] Nucleic acid isolated or synthesized in accordance with the sequences described herein have utility as primers for the amplification of  H. pylori  nucleic acid. These nucleic acids may also have utility as primers for the amplification of nucleic acids in other Helicobacter species. With respect to polymerase chain reaction (PCR) techniques, nucleic acid sequences of ≧10-15 nucleotides of the invention contained in the Sequence Listing have utility in conjunction with suitable enzymes and reagents to create copies of  H. pylori  nucleic acid. More preferably, the sequence will comprise twenty or more nucleotides to convey stability to the hybridization product formed between the primer and the intended target molecules. Binding conditions of primers greater than 100 nucleotides are more difficult to control to obtain specificity. High fidelity PCR can be used to ensure a faithful DNA copy prior to expression. In addition, amplified products can be checked by conventional sequencing methods.  
     [0274] The copies can be used in diagnostic assays to detect specific sequences, including genes from  H. pylori  and/or other Helicobacter species. The copies can also be incorporated into cloning and expression vectors to generate polypeptides corresponding to the nucleic acid synthesized by PCR, as is described in greater detail herein.  
     [0275] Antisense  
     [0276] Nucleic acid or nucleic acid-hybridizing derivatives isolated or synthesized in accordance with the sequences described herein have utility as antisense agents to prevent the expression of  H. pylori  genes. These sequences also have utility as antisense agents to prevent expression of genes of other Helicobacter species.  
     [0277] In one embodiment, nucleic acid or derivatives corresponding to  H. pylori  nucleic acids is loaded into a suitable carrier such as a liposome or bacteriophage for introduction into bacterial cells. For example, a nucleic acid having twenty or more nucleotides is capable of binding to bacteria nucleic acid or bacteria messenger RNA. Preferably, the antisense nucleic acid is comprised of 20 or more nucleotides to provide necessary stability of a hybridization product of non-naturally occurring nucleic acid and bacterial nucleic acid and/or bacterial messenger RNA. Nucleic acid having a sequence greater than 1000 nucleotides in length is difficult to synthesize but can be generated by recombinant DNA techniques. Methods for loading antisense nucleic acid in liposomes is known in the art as exemplified by U.S. Pat. No. 4,241,046 issued Dec. 23, 1980 to Papahadjopoulos et al.  
     [0278] II. Expression of  H. pylori  Nucleic Acids  
     [0279] Nucleic acid isolated or synthesized in accordance with the sequences described herein have utility to generate polypeptides. The nucleic acid of the invention exemplified in the Sequence Listing or fragments of the nucleic acid encoding active portions of  H. pylori  polypeptides can be cloned into suitable vectors or used to isolate nucleic acid. The isolated nucleic acid is combined with suitable DNA linkers and cloned into a suitable vector.  
     [0280] The function of a specific gene or operon can be ascertained by expression in a bacterial strain under conditions where the activity of the gene product(s) specified by the gene or operon in question can be specifically measured. Alternatively, a gene product may be produced in large quantities in an expressing strain for use as an antigen, an industrial reagent, for structural studies, etc. This expression can be accomplished in a mutant strain which lacks the activity of the gene to be tested, or in a strain that does not produce the same gene product(s). This includes, but is not limited to other Helicobacter strains, or other bacterial strains such as  E coli , Norcardia, Corynebacterium, Campylobacter, and Streptomyces species. In some cases the expression host will utilize the natural Helicobacter promoter whereas in others, it will be necessary to drive the gene with a promoter sequence derived from the expressing organism (e.g., an  E. coli  beta-galactosidase promoter for expression in  E coli ).  
     [0281] To express a gene product using the natural  H. pylori  promoter, a procedure such as the following can be used. A restriction fragment containing the gene of interest, together with its associated natural promoter element and regulatory sequences (identified using the DNA sequence data) is cloned into an appropriate recombinant plasmid containing an origin of replication that functions in the host organism and an appropriate selectable marker. This can be accomplished by a number of procedures known to those skilled in the art. It is most preferably done by cutting the plasmid and the fragment to be cloned with the same restriction enzyme to produce compatible ends that can be ligated to join the two pieces together. The recombinant plasmid is introduced into the host organism by, for example, electroporation and cells containing the recombinant plasmid are identified by selection for the marker on the plasmid. Expression of the desired gene product is detected using an assay specific for that gene product.  
     [0282] In the case of a gene that requires a different promoter, the body of the gene (coding sequence) is specifically excised and cloned into an appropriate expression plasmid. This subcloning can be done by several methods, but is most easily accomplished by PCR amplification of a specific fragment and ligation into an expression plasmid after treating the PCR product with a restriction enzyme or exonuclease to create suitable ends for cloning.  
     [0283] A suitable host cell for expression of a gene can be any procaryotic or eucaryotic cell. For example, an  H. pylori  polypeptide can be expressed in bacterial cells such as  E. coli , insect cells (baculovirus), yeast, or mammalian cells such as Chinese hamster ovary cell (CHO). Other suitable host cells are known to those skilled in the art.  
     [0284] Expression in eucaryotic cells such as mammalian, yeast, or insect cells can lead to partial or complete glycosylation and/or formation of relevant inter- or intra-chain disulfide bonds of a recombinant peptide product. Examples of vectors for expression in yeast  S. cerivisae  include pYepSec1 (Baldari. et al., (1987)  Embo J.  6:229-234), pMFa (Kurjan and Herskowitz, (1982) Cell 30:933-943), pJRY88 (Schultz et al., (1987)  Gene  54:113-123), and pYES2 (Invitrogen Corporation, San Diego, Calif.). Baculovirus vectors available for expression of proteins in cultured insect cells (SF 9 cells) include the pAc series (Smith et al., (1983)  Mol. Cell Biol.  3:2156-2165) and the pVL series (Lucklow, V. A., and Summers, M. D., (1989)  Virology  170:31-39). Generally, COS cells (Gluzman, Y., (1981) Cell 23:175-182) are used in conjunction with such vectors as pCDM 8 (Aruffo, A. and Seed, B., (1987)  Proc. Natl. Acad. Sci. USA  84:8573-8577) for transient amplification/expression in mammalian cells, while CHO (dhfr −  Chinese Hamster Ovary) cells are used with vectors such as pMT2PC (Kaufman et al. (1987),  EMBO J.  6:187-195) for stable amplification/expression in mammalian cells. Vector DNA can be introduced into mammalian cells via conventional techniques such as calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediated transfection, or electroporation. Suitable methods for transforming host cells can be found in Sambrook et al. ( Molecular Cloning: A Laboratory Manual,  2nd Edition, Cold Spring Harbor Laboratory press (1989)), and other laboratory textbooks.  
     [0285] Expression in procaryotes is most often carried out in  E. coli  with either fusion or non-fusion inducible expression vectors. Fusion vectors usually add a number of NH 2  terminal amino acids to the expressed target gene. These NH 2  terminal amino acids often are referred to as a reporter group. Such reporter groups usually serve two purposes: 1) to increase the solubility of the target recombinant protein; and 2) to aid in the purification of the target recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the reporter group and the target recombinant protein to enable separation of the target recombinant protein from the reporter group subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase. Typical fusion expression vectors include pGEX (Amrad Corp., Melbourne, Australia), pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) which fuse glutathione S-transferase, maltose E binding protein, or protein A, respectively, to the target recombinant protein. A preferred reporter group is poly(His), which may be fused to the amino or carboxy terminus of the protein and which renders the recombinant fusion protein easily purifiable by metal chelate chromatography.  
     [0286] Inducible non-fusion expression vectors include pTrc (Amann et al., (1988) Gene 69:301-315) and pET11d (Studier et al.,  Gene Expression Technology: Methods in Enzymology  185, Academic Press, San Diego, Calif. (1990) 60-89). While target gene expression relies on host RNA polymerase transcription from the hybrid trp-lac fusion promoter in pTrc, expression of target genes inserted into pET11d relies on transcription from the T7 gn10-lac 0 fusion promoter mediated by coexpressed viral RNA polymerase (T7 gnl). This viral polymerase is supplied by host strains BL21(DE3) or HMS174(DE3) from a resident λ prophage harboring a T7 gnl under the transcriptional control of the lacUV 5 promoter.  
     [0287] For example, a host cell transfected with a nucleic acid vector directing expression of a nucleotide sequence encoding an  H. pylori  polypeptide can be cultured under appropriate conditions to allow expression of the polypeptide to occur. The polypeptide may be secreted and isolated from a mixture of cells and medium containing the peptide. Alternatively, the polypeptide may be retained cytoplasmically and the cells harvested, lysed and the protein isolated. A cell culture includes host cells, media and other byproducts. Suitable media for cell culture are well known in the art. Polypeptides of the invention can be isolated from cell culture medium, host cells, or both using techniques known in the art for purifying proteins including ion-exchange chromatography, gel filtration chromatography, ultrafiltration, electrophoresis, and immunoaffinity purification with antibodies specific for such polypeptides. Additionally, in many situations, polypeptides can be produced by chemical cleavage of a native protein (e.g., tryptic digestion) and the cleavage products can then be purified by standard techniques.  
     [0288] In the case of membrane bound proteins, these can be isolated from a host cell by contacting a membrane-associated protein fraction with a detergent forming a solubilized complex, where the membrane-associated protein is no longer entirely embedded in the membrane fraction and is solubilized at least to an extent which allows it to be chromatographically isolated from the membrane fraction. Several different criteria are used for choosing a detergent suitable for solubilizing these complexes. For example, one property considered is the ability of the detergent to solubilize the  H. pylori  protein within the membrane fraction at minimal denaturation of the membrane-associated protein allowing for the activity or functionality of the membrane-associated protein to return upon reconstitution of the protein. Another property considered when selecting the detergent is the critical micelle concentration (CMC) of the detergent in that the detergent of choice preferably has a high CMC value allowing for ease of removal after reconstitution. A third property considered when selecting a detergent is the hydrophobicity of the detergent. Typically, membrane-associated proteins are very hydrophobic and therefore detergents which are also hydrophobic, e.g., the triton series, would be useful for solubilizing the hydrophobic proteins. Another property important to a detergent can be the capability of the detergent to remove the  H. pylori  protein with minimal protein-protein interaction facilitating further purification. A fifth property of the detergent which should be considered is the charge of the detergent. For example, if it is desired to use ion exchange resins in the purification process then preferably detergent should be an uncharged detergent. Chromatographic techniques which can be used in the final purification step are known in the art and include hydrophobic interaction, lectin affinity, ion exchange, dye affinity and immunoaffinity.  
     [0289] One strategy to maximize recombinant  H. pylori  peptide expression in  E. coli  is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein (Gottesman, S.,  Gene Expression Technology: Methods in Enzymology  185, Academic Press, San Diego, Calif. (1990) 119-128). Another strategy would be to alter the nucleic acid encoding an  H. pylori  peptide to be inserted into an expression vector so that the individual codons for each amino acid would be those preferentially utilized in highly expressed  E. coli  proteins (Wada et al., (1992)  Nuc. Acids Res.  20:2111-2118). Such alteration of nucleic acids of the invention can be carried out by standard DNA synthesis techniques.  
     [0290] The nucleic acids of the invention can also be chemically synthesized using standard techniques. Various methods of chemically synthesizing polydeoxynucleotides are known, including solid-phase synthesis which, like peptide synthesis, has been fully automated in commercially available DNA synthesizers (See, e.g., Itakura et al. U.S. Pat. No. 4,598,049; Caruthers et al. U.S. Pat. No. 4,458,066; and Itakura U.S. Pat. Nos. 4,401,796 and 4,373,071, incorporated by reference herein).  
     [0291] III.  H. pylori  Polypeptides  
     [0292] This invention encompasses isolated  H. pylori  polypeptides encoded by the disclosed  H. pylori  genomic sequences, including the polypeptides of the invention contained in the Sequence Listing. Polypeptides of the invention are preferably at least 5 amino acid residues in length. Using the DNA sequence information provided herein, the amino acid sequences of the polypeptides encompassed by the invention can be deduced using methods well-known in the art. It will be understood that the sequence of an entire nucleic acid encoding an  H. pylori  polypeptide can be isolated and identified based on an ORF that encodes only a fragment of the cognate protein-coding region. This can be acheived, for example, by using the isolated nucleic acid encoding the ORF, or fragments thereof, to prime a polymerase chain reaction with genomic  H. pylori  DNA as template; this is followed by sequencing the amplified product.  
     [0293] The polypeptides of the invention can be isolated from wild-type or mutant  H. pylori  cells or from heterologous organisms or cells (including, but not limited to, bacteria, yeast, insect, plant and mammalian cells) into which an  H. pylori  nucleic acid has been introduced and expressed. In addition, the polypeptides can be part of recombinant fusion proteins.  
     [0294] H. pylori  polypeptides of the invention can be chemically synthesized using commercially automated procedures such as those referenced herein.  
     [0295] H. pylori  polypeptides of the invention are also intended to include chimeric proteins and truncated proteins as decribed herein.  
     [0296] Chimeric  H. pylori  Proteins  
     [0297] H. pylori  chimeric polypeptides comprise one or more  H. pylori  polypeptides fused together. These combined sequences can be made by combining two or more genes, or two or more polypeptide encoding sequences, or at least one gene and at least one polypeptide encoding sequence in tandem, and the subsequent expression of the encoded proteins by conventional molecular biological techniques. The combined nucleotide sequences may be composed of a combination of either full length  H. pylori  nucleotide sequences or fragments of such sequences, e.g., fragments which contain immunologically relevant portions of the encoded  H. pylori  protein. These chimeric  H. pylori  proteins then contain the combined or synergistic vaccine potential of each individual  H. pylori  protein sequence and can be used in vaccine formulations of the invention.  
     [0298] Truncated Gene Expression and Protein Production  
     [0299] H. pylori  proteins encoded by a given nucleotide sequence can also be used in a biologically active truncated form. Such truncation can be produced, for example, by the elimination of either 5′ and/or 3′ regions of the encoding nucleotide sequence. These truncations can affect recombinant expression of the encoded protein and/or subsequent purification of the protein. For example, truncation of a nucleotide sequence encoding a predicted export sequence of a specific protein may alter expression of the protein. Alternatively, C-terminal truncation of an  H. pylori  polypeptide by elimination of the 3′ end of the nucleic acid coding region may also improve protein expression and subsequent purification and use, as is outlined in Example VIIIb low. Deletion of nucleic acid regions encoding internal  H. pylori  protein regions can also result in improved protein expression, purification and/or efficacy as a vaccine candidate.  
     [0300] IV. Identification of Nucleic Acids Encoding Vaccine Components and Targets for Agents Effective Against  H. pylori    
     [0301] The disclosed  H. pylori  genome sequence includes segments that direct the synthesis of ribonucleic acids and polypeptides, as well as origins of replication, promoters, other types of regulatory sequences, and intergenic nucleic acids. The invention encompasses nucleic acids encoding immunogenic components of vaccines and targets for agents effective against  H. pylori . Identification of said immunogenic components involved in the determination of the function of the disclosed sequences can be achieved using a variety of approaches. Non-limiting examples of these approaches are described briefly below.  
     [0302] Homology to known sequences: Computer-assisted comparison of the disclosed  H. pylori  sequences with previously reported sequences present in publicly available databases is useful for identifying functional  H. pylori  nucleic acid and polypeptide sequences. It will be understood that protein-coding sequences, for example, may be compared as a whole, and that a high degree of sequence homology between two proteins (such as, for example, &gt;80-90%) at the amino acid level indicates that the two proteins also possess some degree of functional homology, such as, for example, among enzymes involved in metabolism, DNA synthesis, or cell wall synthesis, and proteins involved in transport, cell division, etc. In addition, many structural features of particular protein classes have been identified and correlate with specific consensus sequences, such as, for example, binding domains for nucleotides, DNA, metal ions, and other small molecules; sites for covalent modifications such as phosphorylation, acylation, and the like; sites of protein:protein interactions, etc. These consensus sequences may be quite short and thus may represent only a fraction of the entire protein-coding sequence. Identification of such a feature in an  H. pylori  sequence is therefore useful in determining the function of the encoded protein and identifying useful targets of antibacterial drugs.  
     [0303] Of particular relevance to the present invention are structural features that are common to secretory, transmembrane, and surface proteins, including secretion signal peptides and hydrophobic transmembrane domains.  H. pylori  proteins identified as containing putative signal sequences and/or transmembrane domains are useful as immunogenic components of vaccines.  
     [0304] Identification of essential genes: Nucleic acids that encode proteins essential for growth or viability of  H. pylori  are preferred drug targets.  H. pylori  genes can be tested for their biological relevance to the organism by examining the effect of deleting and/or disrupting the genes, i.e., by so-called gene “knockout”, using techniques known to those skilled in the relevant art. In this manner, essential genes may be identified.  
     [0305] Strain-specific sequences: Because of the evolutionary relationship between different  H. pylori  strains, it is believed that the presently disclosed  H. pylori  sequences are useful for identifying, and/or discriminating between, previously known and new  H. pylori  strains. It is believed that other  H. pylori  strains will exhibit at least 70% sequence homology with the presently disclosed sequence. Systematic and routine analyses of DNA sequences derived from samples containing  H. pylori  strains, and comparison with the present sequence allows for the identification of sequences that can be used to discriminate between strains, as well as those that are common to all  H. pylori  strains. In one embodiment, the invention provides nucleic acids, including probes, and peptide and polypeptide sequences that discriminate between different strains of  H. pylori . Strain-specific components can also be identified functionally by their ability to elicit or react with antibodies that selectively recognize one or more  H. pylori  strains.  
     [0306] In another embodiment, the invention provides nucleic acids, including probes, and peptide and polypeptide sequences that are common to all  H. pylori  strains but are not found in other bacterial species.  
     [0307] Specific Example: Determination Of Candidate Protein Antigens For Antibody And Vaccine Development  
     [0308] The selection of candidate protein antigens for vaccine development can be derived from the nucleic acids encoding  H. pylori  polypeptides. First, the ORF&#39;s can be analyzed for homology to other known exported or membrane proteins and analyzed using the discriminant analysis described by Klein, et al. (Klein, P., Kanehsia, M., and DeLisi, C. (1985)  Biochimica et Biophysica Acta  815, 468-476) for predicting exported and membrane proteins.  
     [0309] Homology searches can be performed using the BLAST algorithm contained in the Wisconsin Sequence Analysis Package (Genetics Computer Group, University Research Park, 575 Science Drive, Madison, Wis. 53711) to compare each predicted ORF amino acid sequence with all sequences found in the current GenBank, SWISS-PROT and PIR databases. BLAST searches for local alignments between the ORF and the databank sequences and reports a probability score which indicates the probability of finding this sequence by chance in the database. ORF&#39;s with significant homology (e.g. probabilities lower than 1×10 −6  that the homology is only due to random chance) to membrane or exported proteins represent protein antigens for vaccine development. Possible functions can be provided to  H. pylori  genes based on sequence homology to genes cloned in other organisms.  
     [0310] Discriminant analysis (Klein, et al. supra) can be used to examine the ORF amino acid sequences. This algorithm uses the intrinsic information contained in the ORF amino acid sequence and compares it to information derived from the properties of known membrane and exported proteins. This comparison predicts which proteins will be exported, membrane associated or cytoplasmic. ORF amino acid sequences identified as exported or membrane associated by this algorithm are likely protein antigens for vaccine development.  
     [0311] Surface exposed outer membrane proteins are likely to represent the best antigens to provide a protective immune response against  H. pylori . Among the algorithms that can be used to aid in prediction of these outer membrane proteins include the presence of an amphipathic beta-sheet region at their C-terminus. This region which has been detected in a large number of outer membrane proteins in Gram negative bacteria is often characterized by hydrophobic residues (Phe or Tyr) approximately at positions 1, 3, 5, 7 and 9 from the C-terminus (e.g., see FIGS.  10 - 12 , block F). In many of these figures of multiple sequence alignments, an asterisk is used to denote amino acid residues shared by all members of the group, and a dot is used to indicate that all members of the group share homologous amino acid residues at that position. Importantly, these sequences have not been detected at the C-termini of periplasmic proteins, thus allowing preliminary distinction between these classes of proteins based on primary sequence data. This phenomenon has been reported previously by Struyve et al. ( J. Mol. Biol.  218:141-148, 1991).  
     [0312] Also illustrated in FIGS.  10 - 12  and in FIG. 13 are additional amino acid sequence motifs found in many outer membrane proteins of  H. pylori . The amino acid sequence alignments in FIGS.  10 - 13  depict portions of the sequence of twenty-three  H. pylori  proteins (depicted in the single letter amino acid code) labeled with arbitrary names and their amino acid Sequence ID Numbers and shown N-terminal to C-terminal, left to right. Six distinct blocks (labeled A through F) of similar amino acid residues are found including the distinctive hydrophobic residues (Phe or Tyr; F or Y according to the single letter code for amino acid residues) frequently found at positions near the C-terminus of outer membrane proteins. The presence of several shared motifs clearly establishes the similarity between members of this group of proteins.  
     [0313] In addition, outer membrane proteins isolated from  H. pylori  frequently share a motif near the mature N-terminus (i.e., after processing to remove the secretion signal) as illustrated in the blocked amino acid residues in FIG. 4. FIG. 14 depicts the N-terminal portion of six  H. pylori  proteins (designated by arbitrary names and their amino acid Sequence ID Numbers and shown N-terminal to C-terminal, left to right). Varied groups of outer membrane proteins share significant homology across most of their sequences. Examples of such protein families are shown in FIGS.  15 - 17  (proteins are designated by an arbitrary name and their amino acid Sequence ID Numbers and shown N-terminal to C-terminal, left to right).  
     [0314] One skilled in the art would know that these shared sequence motifs are highly significant and establish a similarity among this group of proteins.  
     [0315] Infrequently it is not possible to distinguish between multiple possible nucleotides at a given position in the nucleic acid sequence. In those cases the ambiguities are denoted by an extended alphabet as follows:  
     [0316] These are the official IUPAC-IUB single-letter base codes  
                                   Code   Base Description                                            G   Guanine           A   Adenine       T   Thymine       C   Cytosine       R   Purine   (A or G)       Y   Pyrimidine   (C or T or U)       M   Amino   (A or C)       K   Ketone   (G or T)       S   Strong interaction   (C or G)       W   Weak interaction   (A or T)       H   Not-G   (A or C or T)       B   Not-A   (C or G or T)       V   Not-T (not-U)   (A or C or G)       D   Not-C   (A or G or T)       N   Any   (A or C or G or T)                  
 
     [0317] The amino acid translations of this invention account for the ambiguity in the nucleic acid sequence by translating the ambiguous codon as the letter “X”. In all cases, the permissible amino acid residues at a position are clear from an examination of the nucleic acid sequence based on the standard genetic code.  
     [0318] V. Production of Fragments and Analogs of  H. pylori Nucleic Acids and Polypeptides  
     [0319] Based on the discovery of the  H. pylori  gene products of the invention provided in the Sequence Lsiting, one skilled in the art can alter the disclosed structure (of  H. pylori  genes), e.g., by producing fragments or analogs and test the newly produced structures for activity. Examples of techniques known to those skilled in the relevant art which allow the production and testing of fragments and analogs are discussed below. These, or analogous methods can be used to make and screen libraries of polypeptides, e.g., libraries of random peptides or libraries of fragments or analogs of cellular proteins for the ability to bind  H. pylori  polypeptides. Such screens are useful for the identification of inhibitors of  H. pylori.    
     [0320] Generation of Fragments  
     [0321] Fragments of a protein can be produced in several ways, e.g., recombinantly, by proteolytic digestion, or by chemical synthesis. Internal or terminal fragments of a polypeptide can be generated by removing one or more nucleotides from one end (for a terminal fragment) or both ends (for an internal fragment) of a nucleic acid which encodes the polypeptide. Expression of the mutagenized DNA produces polypeptide fragments. Digestion with “end-nibbling” endonucleases can thus generate DNA&#39;s which encode an array of fragments. DNA&#39;s which encode fragments of a protein can also be generated by random shearing, restriction digestion or a combination of the above-discussed methods.  
     [0322] Fragments can also be chemically synthesized using techniques known in the art such as conventional Merrifield solid phase f-Moc or t-Boc chemistry. For example, peptides of the present invention may be arbitrarily divided into fragments of desired length with no overlap of the fragments, or divided into overlapping fragments of a desired length.  
     [0323] Alteration of Nucleic Acids and Polypeptides: Random Methods  
     [0324] Amino acid sequence variants of a protein can be prepared by random mutagenesis of DNA which encodes a protein or a particular domain or region of a protein. Useful methods include PCR mutagenesis and saturation mutagenesis. A library of random amino acid sequence variants can also be generated by the synthesis of a set of degenerate oligonucleotide sequences. (Methods for screening proteins in a library of variants are elsewhere herein).  
     [0325] (A) PCR Mutagenesis  
     [0326] In PCR mutagenesis, reduced Taq polymerase fidelity is used to introduce random mutations into a cloned fragment of DNA (Leung et al., 1989,  Technique  1: 11-15). The DNA region to be mutagenized is amplified using the polymerase chain reaction (PCR) under conditions that reduce the fidelity of DNA synthesis by Taq DNA polymerase, e.g., by using a dGTP/dATP ratio of five and adding Mn 2+  to the PCR reaction. The pool of amplified DNA fragments are inserted into appropriate cloning vectors to provide random mutant libraries.  
     [0327] (B) Saturation Mutagenesis  
     [0328] Saturation mutagenesis allows for the rapid introduction of a large number of single base substitutions into cloned DNA fragments (Mayers et al., 1985,  Science  229:242). This technique includes generation of mutations, e.g., by chemical treatment or irradiation of single-stranded DNA in vitro, and synthesis of a complimentary DNA strand. The mutation frequency can be modulated by modulating the severity of the treatment, and essentially all possible base substitutions can be obtained. Because this procedure does not involve a genetic selection for mutant fragments both neutral substitutions, as well as those that alter function, are obtained. The distribution of point mutations is not biased toward conserved sequence elements.  
     [0329] (C) Degenerate Oligonucleotides  
     [0330] A library of homologs can also be generated from a set of degenerate oligonucleotide sequences. Chemical synthesis of a degenerate sequences can be carried out in an automatic DNA synthesizer, and the synthetic genes then ligated into an appropriate expression vector. The synthesis of degenerate oligonucleotides is known in the art (see for example, Narang, S A (1983)  Tetrahedron  39:3; Itakura et al. (1981)  Recombinant DNA, Proc  3 rd Cleveland Sympos. Macromolecules , ed. AG Walton, Amsterdam: Elsevier pp273-289; Itakura et al. (1984)  Annu. Rev. Biochem.  53:323; Itakura et al. (1984)  Science  198:1056; Ike et al. (1983)  Nucleic Acid Res.  11:477. Such techniques have been employed in the directed evolution of other proteins (see, for example, Scott et al. (1990)  Science  249:386-390; Roberts et al. (1992) PNAS 89:2429-2433; Devlin et al. (1990)  Science  249: 404-406; Cwirla et al. (1990)  PNAS  87: 6378-6382; as well as U.S. Pat. Nos. 5,223,409, 5,198,346, and 5,096,815).  
     [0331] Alteration of Nucleic Acids and Polypeptides: Methods for Directed Mutagenesis  
     [0332] Non-random or directed, mutagenesis techniques can be used to provide specific sequences or mutations in specific regions. These techniques can be used to create variants which include, e.g., deletions, insertions, or substitutions, of residues of the known amino acid sequence of a protein. The sites for mutation can be modified individually or in series, e.g., by (1) substituting first with conserved amino acids and then with more radical choices depending upon results achieved, (2) deleting the target residue, or (3) inserting residues of the same or a different class adjacent to the located site, or combinations of options 1-3.  
     [0333] (A) Alanine Scanning Mutagenesis  
     [0334] Alanine scanning mutagenesis is a useful method for identification of certain residues or regions of the desired protein that are preferred locations or domains for mutagenesis, Cunningham and Wells ( Science  244:1081-1085, 1989). In alanine scanning, a residue or group of target residues are identified (e.g., charged residues such as Arg, Asp, His, Lys, and Glu) and replaced by a neutral or negatively charged amino acid (most preferably alanine or polyalanine). Replacement of an amino acid can affect the interaction of the amino acids with the surrounding aqueous environment in or outside the cell. Those domains demonstrating functional sensitivity to the substitutions are then refined by introducing further or other variants at or for the sites of substitution. Thus, while the site for introducing an amino acid sequence variation is predetermined, the nature of the mutation per se need not be predetermined. For example, to optimize the performance of a mutation at a given site, alanine scanning or random mutagenesis may be conducted at the target codon or region and the expressed desired protein subunit variants are screened for the optimal combination of desired activity.  
     [0335] (B) Oligonucleotide-Mediated Mutagenesis  
     [0336] Oligonucleotide-mediated mutagenesis is a useful method for preparing substitution, deletion, and insertion variants of DNA, see, e.g., Adelman et al., ( DNA  2:183, 1983). Briefly, the desired DNA is altered by hybridizing an oligonucleotide encoding a mutation to a DNA template, where the template is the single-stranded form of a plasmid or bacteriophage containing the unaltered or native DNA sequence of the desired protein. After hybridization, a DNA polymerase is used to synthesize an entire second complementary strand of the template that will thus incorporate the oligonucleotide primer, and will code for the selected alteration in the desired protein DNA. Generally, oligonucleotides of at least 25 nucleotides in length are used. An optimal oligonucleotide will have 12 to 15 nucleotides that are completely complementary to the template on either side of the nucleotide(s) coding for the mutation. This ensures that the oligonucleotide will hybridize properly to the single-stranded DNA template molecule. The oligonucleotides are readily synthesized using techniques known in the art such as that described by Crea et al. ( Proc. Natl. Acad. Sci . USA, 75: 5765 [1978]).  
     [0337] (C) Cassette Mutagenesis  
     [0338] Another method for preparing variants, cassette mutagenesis, is based on the technique described by Wells et al. ( Gene,  34:315[1985]). The starting material is a plasmid (or other vector) which includes the protein subunit DNA to be mutated. The codon(s) in the protein subunit DNA to be mutated are identified. There must be a unique restriction endonuclease site, on each side of the identified mutation site(s). If no such restriction sites exist, they may be generated using the above-described oligonucleotide-mediated mutagenesis method to introduce them at appropriate locations in the desired protein subunit DNA. After the restriction sites have been introduced into the plasmid, the plasmid is cut at these sites to linearize it. A double-stranded oligonucleotide encoding the sequence of the DNA between the restriction sites but containing the desired mutation(s) is synthesized using standard procedures. The two strands are synthesized separately and then hybridized together using standard techniques. This double-stranded oligonucleotide is referred to as the cassette. This cassette is designed to have 3′ and 5′ ends that are comparable with the ends of the linearized plasmid, such that it can be directly ligated to the plasmid. This plasmid now contains the mutated desired protein subunit DNA sequence.  
     [0339] (D) Combinatorial Mutagenesis  
     [0340] Combinatorial mutagenesis can also be used to generate mutants (Ladner et al., WO 88/06630). In this method, the amino acid sequences for a group of homologs or other related proteins are aligned, preferably to promote the highest homology possible. All of the amino acids which appear at a given position of the aligned sequences can be selected to create a degenerate set of combinatorial sequences. The variegated library of variants is generated by combinatorial mutagenesis at the nucleic acid level, and is encoded by a variegated gene library. For example, a mixture of synthetic oligonucleotides can be enzymatically ligated into gene sequences such that the degenerate set of potential sequences are expressible as individual peptides, or alternatively, as a set of larger fusion proteins containing the set of degenerate sequences.  
     [0341] Other Modifications of H. pylori Nucleic Acids and Polypeptides  
     [0342] It is possible to modify the structure of an  H. pylori  polypeptide for such purposes as increasing solubility, enhancing stability (e.g., shelf life ex vivo and resistance to proteolytic degradation in vivo). A modified  H. pylori  protein or peptide can be produced in which the amino acid sequence has been altered, such as by amino acid substitution, deletion, or addition as described herein.  
     [0343] An  H. pylori  peptide can also be modified by substitution of cysteine residues preferably with alanine, serine, threonine, leucine or glutamic acid residues to minimize dimerization via disulfide linkages. In addition, amino acid side chains of fragments of the protein of the invention can be chemically modified. Another modification is cyclization of the peptide.  
     [0344] In order to enhance stability and/or reactivity, an  H. pylori  polypeptide can be modified to incorporate one or more polymorphisms in the amino acid sequence of the protein resulting from any natural allelic variation. Additionally, D-amino acids, non-natural amino acids, or non-amino acid analogs can be substituted or added to produce a modified protein within the scope of this invention. Furthermore, an  H. pylori  polypeptide can be modified using polyethylene glycol (PEG) according to the method of A. Sehon and co-workers (Wie et al., supra) to produce a protein conjugated with PEG. In addition, PEG can be added during chemical synthesis of the protein. Other modifications of  H. pylori  proteins include reduction/alkylation (Tarr,  Methods of Protein Microcharacterization , J. E. Silver ed., Humana Press, Clifton N.J. 155-194 (1986)); acylation (Tarr, supra); chemical coupling to an appropriate carrier (Mishell and Shiigi, eds,  Selected Methods in Cellular Immunology , W H Freeman, San Francisco, Calif. (1980), U.S. Pat. No. 4,939,239; or mild formalin treatment (Marsh, (1971)  Int. Arch. of Allergy and Appl. Immunol.,  41: 199-215).  
     [0345] To facilitate purification and potentially increase solubility of an  H. pylori  protein or peptide, it is possible to add an amino acid fusion moiety to the peptide backbone. For example, hexa-histidine can be added to the protein for purification by immobilized metal ion affinity chromatography (Hochuli, E. et al., (1988)  Bio/Technology,  6: 1321-1325). In addition, to facilitate isolation of peptides free of irrelevant sequences, specific endoprotease cleavage sites can be introduced between the sequences of the fusion moiety and the peptide.  
     [0346] To potentially aid proper antigen processing of epitopes within an  H. pylori  polypeptide, canonical protease sensitive sites can be engineered between regions, each comprising at least one epitope via recombinant or synthetic methods. For example, charged amino acid pairs, such as KK or RR, can be introduced between regions within a protein or fragment during recombinant construction thereof. The resulting peptide can be rendered sensitive to cleavage by cathepsin and/or other trypsin-like enzymes which would generate portions of the protein containing one or more epitopes. In addition, such charged amino acid residues can result in an increase in the solubility of the peptide.  
     [0347] Primary Methods for Screening Polypeptides and Analogs  
     [0348] Various techniques are known in the art for screening generated mutant gene products. Techniques for screening large gene libraries often include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of vectors, and expressing the genes under conditions in which detection of a desired activity, e.g., in this case, binding to  H. pylori  polypeptide or an interacting protein, facilitates relatively easy isolation of the vector encoding the gene whose product was detected. Each of the techniques described below is amenable to high through-put analysis for screening large numbers of sequences created, e.g., by random mutagenesis techniques.  
     [0349] (A) Two Hybrid Systems  
     [0350] Two hybrid assays such as the system described above (as with the other screening methods described herein), can be used to identify polypeptides, e.g., fragments or analogs of a naturally-occurring  H. pylori  polypeptide, e.g., of cellular proteins, or of randomly generated polypeptides which bind to an  H. pylori  protein. (The  H. pylori  domain is used as the bait protein and the library of variants are expressed as fish fusion proteins.) In an analogous fashion, a two hybrid assay (as with the other screening methods described herein), can be used to find polypeptides which bind a  H. pylori  polypeptide.  
     [0351] (B) Display Libraries  
     [0352] In one approach to screening assays, the candidate peptides are displayed on the surface of a cell or viral particle, and the ability of particular cells or viral particles to bind an appropriate receptor protein via the displayed product is detected in a “panning assay”. For example, the gene library can be cloned into the gene for a surface membrane protein of a bacterial cell, and the resulting fusion protein detected by panning (Ladner et al., WO 88/06630; Fuchs et al. (1991)  Bio/Technology  9:1370-1371; and Goward et al. (1992)  TIBS  18:136-140). In a similar fashion, a detectably labeled ligand can be used to score for potentially functional peptide homologs. Fluorescently labeled ligands, e.g., receptors, can be used to detect homologs which retain ligand-binding activity. The use of fluorescently labeled ligands, allows cells to be visually inspected and separated under a fluorescence microscope, or, where the morphology of the cell permits, to be separated by a fluorescence-activated cell sorter.  
     [0353] A gene library can be expressed as a fusion protein on the surface of a viral particle. For instance, in the filamentous phage system, foreign peptide sequences can be expressed on the surface of infectious phage, thereby conferring two significant benefits. First, since these phage can be applied to affinity matrices at concentrations well over 10 13  phage per milliliter, a large number of phage can be screened at one time. Second, since each infectious phage displays a gene product on its surface, if a particular phage is recovered from an affinity matrix in low yield, the phage can be amplified by another round of infection. The group of almost identical  E. coli  filamentous phages M13, fd., and fl are most often used in phage display libraries. Either of the phage gIII or gVIII coat proteins can be used to generate fusion proteins without disrupting the ultimate packaging of the viral particle. Foreign epitopes can be expressed at the NH 2 -terminal end of pIII and phage bearing such epitopes recovered from a large excess of phage lacking this epitope (Ladner et al. PCT publication WO 90/02909; Garrard et al., PCT publication WO 92/09690; Marks et al. (1992)  J. Biol. Chem.  267:16007-16010; Griffiths et al. (1993)  EMBO J.  12:725-734; Clackson et al. (1991)  Nature  352:624-628; and Barbas et al. (1992)  PNAS  89:4457-4461).  
     [0354] A common approach uses the maltose receptor of  E. coli  (the outer membrane protein, LamB) as a peptide fusion partner (Charbit et al. (1986)  EMBO  5, 3029-3037). Oligonucleotides have been inserted into plasmids encoding the LamB gene to produce peptides fused into one of the extracellular loops of the protein. These peptides are available for binding to ligands, e.g., to antibodies, and can elicit an immune response when the cells are administered to animals. Other cell surface proteins, e.g., OmpA (Schorr et al. (1991)  Vaccines  91, pp. 387-392), PhoE (Agterberg, et al. (1990)  Gene  88, 37-45), and PAL (Fuchs et al. (1991)  Bio/Tech  9, 1369-1372), as well as large bacterial surface structures have served as vehicles for peptide display. Peptides can be fused to pilin, a protein which polymerizes to form the pilus-a conduit for interbacterial exchange of genetic information (Thiry et al. (1989)  Appl. Environ. Microbiol.  55, 984-993). Because of its role in interacting with other cells, the pilus provides a useful support for the presentation of peptides to the extracellular environment. Another large surface structure used for peptide display is the bacterial motive organ, the flagellum. Fusion of peptides to the subunit protein flagellin offers a dense array of many peptide copies on the host cells (Kuwajima et al. (1988)  Bio/Tech.  6, 1080-1083). Surface proteins of other bacterial species have also served as peptide fusion partners. Examples include the Staphylococcus protein A and the outer membrane IgA protease of Neisseria (Hansson et al. (1992)  J. Bacteriol.  174, 4239-4245 and Klauser et al. (1990)  EMBO J.  9, 1991-1999).  
     [0355] In the filamentous phage systems and the LamB system described above, the physical link between the peptide and its encoding DNA occurs by the containment of the DNA within a particle (cell or phage) that carries the peptide on its surface. Capturing the peptide captures the particle and the DNA within. An alternative scheme uses the DNA-binding protein LacI to form a link between peptide and DNA (Cull et al. (1992)  PNAS USA  89:1865-1869). This system uses a plasmid containing the LacI gene with an oligonucleotide cloning site at its 3′-end. Under the controlled induction by arabinose, a LacI-peptide fusion protein is produced. This fusion retains the natural ability of LacI to bind to a short DNA sequence known as LacO operator (LacO). By installing two copies of LacO on the expression plasmid, the LacI-peptide fusion binds tightly to the plasmid that encoded it. Because the plasmids in each cell contain only a single oligonucleotide sequence and each cell expresses only a single peptide sequence, the peptides become specifically and stably associated with the DNA sequence that directed its synthesis. The cells of the library are gently lysed and the peptide-DNA complexes are exposed to a matrix of immobilized receptor to recover the complexes containing active peptides. The associated plasmid DNA is then reintroduced into cells for amplification and DNA sequencing to determine the identity of the peptide ligands. As a demonstration of the practical utility of the method, a large random library of dodecapeptides was made and selected on a monoclonal antibody raised against the opioid peptide dynorphin B. A cohort of peptides was recovered, all related by a consensus sequence corresponding to a six-residue portion of dynorphin B. (Cull et al. (1992)  Proc. Natl. Acad. Sci. USA.  89-1869)  
     [0356] This scheme, sometimes referred to as peptides-on-plasmids, differs in two important ways from the phage display methods. First, the peptides are attached to the C-terminus of the fusion protein, resulting in the display of the library members as peptides having free carboxy termini. Both of the filamentous phage coat proteins, pill and pVIII, are anchored to the phage through their C-termini, and the guest peptides are placed into the outward-extending N-terminal domains. In some designs, the phage-displayed peptides are presented right at the amino terminus of the fusion protein. (Cwirla, et al. (1990)  Proc. Natl. Acad. Sci. U.S.A.  87, 6378-6382) A second difference is the set of biological biases affecting the population of peptides actually present in the libraries. The LacI fusion molecules are confined to the cytoplasm of the host cells. The phage coat fusions are exposed briefly to the cytoplasm during translation but are rapidly secreted through the inner membrane into the periplasmic compartment, remaining anchored in the membrane by their C-terminal hydrophobic domains, with the N-termini, containing the peptides, protruding into the periplasm while awaiting assembly into phage particles. The peptides in the LacI and phage libraries may differ significantly as a result of their exposure to different proteolytic activities. The phage coat proteins require transport across the inner membrane and signal peptidase processing as a prelude to incorporation into phage. Certain peptides exert a deleterious effect on these processes and are underrepresented in the libraries (Gallop et al. (1994)  J. Med. Chem.  37(9):1233- 125 I). These particular biases are not a factor in the LacI display system.  
     [0357] The number of small peptides available in recombinant random libraries is enormous. Libraries of 10 7 -10 9  independent clones are routinely prepared. Libraries as large as 10 11  recombinants have been created, but this size approaches the practical limit for clone libraries. This limitation in library size occurs at the step of transforming the DNA containing randomized segments into the host bacterial cells. To circumvent this limitation, an in vitro system based on the display of nascent peptides in polysome complexes has recently been developed. This display library method has the potential of producing libraries 3-6 orders of magnitude larger than the currently available phage/phagemid or plasmid libraries. Furthermore, the construction of the libraries, expression of the peptides, and screening, is done in an entirely cell-free format.  
     [0358] In one application of this method (Gallop et al. (1994)  J. Med. Chem.  37(9):1233-1251), a molecular DNA library encoding 10 12  decapeptides was constructed and the library expressed in an  E. coli  S30 in vitro coupled transcription/translation system. Conditions were chosen to stall the ribosomes on the mRNA, causing the accumulation of a substantial proportion of the RNA in polysomes and yielding complexes containing nascent peptides still linked to their encoding RNA. The polysomes are sufficiently robust to be affinity purified on immobilized receptors in much the same way as the more conventional recombinant peptide display libraries are screened. RNA from the bound complexes is recovered, converted to cDNA, and amplified by PCR to produce a template for the next round of synthesis and screening. The polysome display method can be coupled to the phage display system. Following several rounds of screening, cDNA from the enriched pool of polysomes was cloned into a phagemid vector. This vector serves as both a peptide expression vector, displaying peptides fused to the coat proteins, and as a DNA sequencing vector for peptide identification. By expressing the polysome-derived peptides on phage, one can either continue the affinity selection procedure in this format or assay the peptides on individual clones for binding activity in a phage ELISA, or for binding specificity in a completion phage ELISA (Barret, et al. (1992)  Anal. Biochem  204,357-364). To identify the sequences of the active peptides one sequences the DNA produced by the phagemid host.  
     [0359] Secondary Screening of Polypeptides and Analogs  
     [0360] The high through-put assays described above can be followed by secondary screens in order to identify further biological activities which will, e.g., allow one skilled in the art to differentiate agonists from antagonists. The type of a secondary screen used will depend on the desired activity that needs to be tested. For example, an assay can be developed in which the ability to inhibit an interaction between a protein of interest and its respective ligand can be used to identify antagonists from a group of peptide fragments isolated though one of the primary screens described above.  
     [0361] Therefore, methods for generating fragments and analogs and testing them for activity are known in the art. Once the core sequence of interest is identified, it is routine for one skilled in the art to obtain analogs and fragments.  
     [0362] Peptide Mimetics of  H. pylori  Polypeptides  
     [0363] The invention also provides for reduction of the protein binding domains of the subject  H. pylori  polypeptides to generate mimetics, e.g. peptide or non-peptide agents. The peptide mimetics are able to disrupt binding of a polypeptide to its counter ligand, e.g., in the case of an  H. pylori  polypeptide binding to a naturally occurring ligand. The critical residues of a subject  H. pylori  polypeptide which are involved in molecular recognition of a polypeptide can be determined and used to generate  H. pylori -derived peptidomimetics which competitively or noncompetitively inhibit binding of the  H. pylori  polypeptide with an interacting polypeptide (see, for example, European patent applications EP-412,762A and EP-B31,080A).  
     [0364] For example, scanning mutagenesis can be used to map the amino acid residues of a particular  H. pylori  polypeptide involved in binding an interacting polypeptide, peptidomimetic compounds (e.g. diazepine or isoquinoline derivatives) can be generated which mimic those residues in binding to an interacting polypeptide, and which therefore can inhibit binding of an  H. pylori  polypeptide to an interacting polypeptide and thereby interfere with the function of  H. pylori  polypeptide. For instance, non-hydrolyzable peptide analogs of such residues can be generated using benzodiazepine (e.g., see Freidinger et al. in  Peptides: Chemistry and Biology , G. R. Marshall ed., ESCOM Publisher: Leiden, Netherlands, 1988), azepine (e.g., see Huffman et al. in  Peptides: Chemistry and Biology , G. R. Marshall ed., ESCOM Publisher: Leiden, Netherlands, 1988), substituted gama lactam rings (Garvey et al. in  Peptides. Chemistry and Biology , G. R. Marshall ed., ESCOM Publisher: Leiden, Netherlands, 1988), keto-methylene pseudopeptides (Ewenson et al. (1986)  J Med Chem  29:295; and Ewenson et al. in  Peptides: Structure and Function  (Proceedings of the 9th American Peptide Symposium) Pierce Chemical Co. Rockland, Ill., 1985), β-turn dipeptide cores (Nagai et al. (1985)  Tetrahedron Lett  26:647; and Sato et al. (1986)  J Chem Soc Perkin Trans  1:1231), and β-aminoalcohols (Gordon et al. (1985)  Biochem Biophys Res Commun 126:419; and Dann et al. (1986)  Biochem Biophys Res Commun  134:71).  
     [0365] VI. Vaccine Formulations for  H. pylori  Nucleic Acids and Polypeptides  
     [0366] This invention also features vaccine compositions or formulations (used interchangeably herein) for protection against infection by  H. pylori  or for treatment of  H. pylori  infection. As used herein, the term “treatment of  H. pylori  infection” refers to therapeutic treatment of an existing or established  H pylori  infection. The terms “protection against  H. pylori  infection” or “prophylactic treatment” refer to the use of  H. pylori  vaccine formulation for reducing the risk of or preventing an infection in a subject at risk for  H. pylori  infection. In one embodiment, the vaccine compositions contain one or more immunogenic components, such as a surface protein, from  H. pylori , or portion thereof, and a pharmaceutically acceptable carrier. For example, in one embodiment, the vaccine formulations of the invention contain at least one or combination of  H. pylori  polypeptides or fragments thereof, from same or different  H. pylori  antigens. Nucleic acids and  H. pylori  polypeptides for use in the vaccine formulations of the invention include the nucleic acids and polypeptides set forth in the Sequence Listing, preferably those  H. pylori  nucleic acids that encode surface proteins and surface proteins or fragments thereof. For example, a preferred nucleic acid and  H. pylori  polypeptide for use in a vaccine composition of the invention is selected from the group of nucleic acids which encode cell envelope proteins and  H. pylori  cell envelope proteins as set forth in Table 1. However, any nucleic acid encoding an immunogenic  H. pylori  protein and  H. pylori  polypetide, or portion thereof, can be used in the present invention. These vaccines have therapeutic and/or prophylactic utilities.  
     [0367] One aspect of the invention provides a vaccine composition for protection against infection by  H. pylori  which contains at least one immunogenic fragment of an  H. pylori  protein and a pharmaceutically acceptable carrier. Preferred fragments include peptides of at least about 10 amino acid residues in length, preferably about 10-20 amino acid residues in length, and more preferably about 12-16 amino acid residues in length.  
     [0368] Immunogenic components of the invention can be obtained, for example, by screening polypeptides recombinantly produced from the corresponding fragment of the nucleic acid encoding the full-length  H. pylori  protein. In addition, fragments can be chemically synthesized using techniques known in the art such as conventional Merrifield solid phase f-Moc or t-Boc chemistry.  
     [0369] In one embodiment, immunogenic components are identified by the ability of the peptide to stimulate T cells. Peptides which stimulate T cells, as determined by, for example, T cell proliferation or cytokine secretion are defined herein as comprising at least one T cell epitope. T cell epitopes are believed to be involved in initiation and perpetuation of the immune response to the protein allergen which is responsible for the clinical symptoms of allergy. These T cell epitopes are thought to trigger early events at the level of the T helper cell by binding to an appropriate HLA molecule on the surface of an antigen presenting cell, thereby stimulating the T cell subpopulation with the relevant T cell receptor for the epitope. These events lead to T cell proliferation, lymphokine secretion, local inflammatory reactions, recruitment of additional immune cells to the site of antigen/T cell interaction, and activation of the B cell cascade, leading to the production of antibodies. A T cell epitope is the basic element, or smallest unit of recognition by a T cell receptor, where the epitope comprises amino acids essential to receptor recognition (e.g., approximately 6 or 7 amino acid residues). Amino acid sequences which mimic those of the T cell epitopes are within the scope of this invention.  
     [0370] In another embodiment, immunogenic components of the invention are identified through genomic vaccination. The basic protocol is based on the idea that expression libraries consisting of all or parts of a pathogen genome, e.g., an  H. pylori  genome, can confer protection when used to genetically immunize a host. This expression library immunization (ELI) is analogous to expression cloning and involves reducing a genomic expression library of a pathogen, e.g.,  H. pylori , into plasmids that can act as genetic vaccines. The plasmids can also be designed to encode genetic adjuvants which can dramatically stimulate the humoral response. These genetic adjuvants can be introduced at remote sites and act as well extracelluraly as intracellularly.  
     [0371] This is a new approach to vaccine production that has many of the advantages of live/attenuated pathogens but no risk of infection. An expression library of pathogen DNA is used to immunize a host thereby producing the effects of antigen presentation of a live vaccine without the risk. For example, in the present invention, random fragments from the  H. pylori  genome or from cosmid or plasmid clones, as well as PCR products from genes identified by genomic sequencing, can be used to immunize a host. The feasibility of this approach has been demonstrated with  Mycoplasma pulmonis  (Barry et al.,  Nature  377:632-635, 1995), where even partial expression libraries of  Mycoplasma pulnionis , a natural pathogen in rodents, provided protection against challenge from the pathogen.  
     [0372] ELI is a technique that allows for production of a non-infectious multipartite vaccine, even when little is known about pathogen&#39;s biology, because ELI uses the immune system to screen candidate genes. Once isolated, these genes can be used as genetic vaccines or for development of recombinant protein vaccines. Thus, ELI allows for production of vaccines in a systematic, largely mechanized fashion.  
     [0373] Screening immunogenic components can be accomplished using one or more of several different assays. For example, in vitro, peptide T cell stimulatory activity is assayed by contacting a peptide known or suspected of being immunogenic with an antigen presenting cell which presents appropriate MHC molecules in a T cell culture. Presentation of an immunogenic  H. pylori  peptide in association with appropriate MHC molecules to T cells in conjunction with the necessary costimulation has the effect of transmitting a signal to the T cell that induces the production of increased levels of cytokines, particularly of interleukin-2 and interleukin-4. The culture supernatant can be obtained and assayed for interleukin-2 or other known cytokines. For example, any one of several conventional assays for interleukin-2 can be employed, such as the assay described in  Proc. Natl. Acad. Sci USA,  86: 1333 (1989) the pertinent portions of which are incorporated herein by reference. A kit for an assay for the production of interferon is also available from Genzyme Corporation (Cambridge, Mass.).  
     [0374] Alternatively, a common assay for T cell proliferation entails measuring tritiated thymidine incorporation. The proliferation of T cells can be measured in vitro by determining the amount of  3 H-labeled thymidine incorporated into the replicating DNA of cultured cells. Therefore, the rate of DNA synthesis and, in turn, the rate of cell division can be quantified.  
     [0375] Vaccine compositions or formulations of the invention containing one or more immunogenic components (e.g.,  H. pylori  polypeptide or fragment thereof or nucleic acid encoding an  H. pylori  polypeptide or fragment thereof) preferably include a pharmaceutically acceptable carrier. The term “pharmaceutically acceptable carrier” is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration. Suitable pharmaceutically acceptable carriers include, for example, one or more of water, saline, phosphate buffered saline, dextrose, glycerol, ethanol and the like, as well as combinations thereof. Pharmaceutically acceptable carriers may further comprise minor amounts of auxiliary substances such as wetting or emulsifying agents, preservatives or buffers, which enhance the shelf life or effectiveness of the  H. pylori  nucleic acid or polypeptide. For vaccine formulations of the invention containing  H. pylori  polypeptides, the polypeptide is preferably coadministered with a suitable adjuvant and/or a delivery system described herein.  
     [0376] It will be apparent to those of skill in the art that the therapeutically effective amount of DNA or protein of this invention will depend, inter alia, upon the administration schedule, the unit dose of an  H. pylori  nucleic acid or polypeptide administered, whether the protein or nucleic acid is administered in combination with other therapeutic agents, the immune status and health of the patient, and the therapeutic activity of the particular protein or nucleic acid.  
     [0377] Vaccine formulations are conventionally administered parenterally, e.g., by injection, either subcutaneously or intramuscularly. Methods for intramuscular immunization are described by Wolff et al. (1990)  Science  247: 1465-1468 and by Sedegah et al. (1994)  Immunology  91: 9866-9870. Other modes of administration include oral and pulmonary formulations, suppositories, and transdermal applications. Oral immunization is preferred over parenteral methods for inducing protection against infection by  H. pylori . Czinn et. al. (1993)  Vaccine  11: 637-642. Oral formulations include such normally employed excipients as, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, magnesium carbonate, and the like.  
     [0378] In one embodiment, the vaccine formulation includes, as a pharmaceutically acceptable carrier, an adjuvant. Examples of the suitable adjuvants for use in the vaccine formulations of the invention include, but are not limited, to aluminum hydroxide; N-acetyl-muramyl—L-threonyl-D-isoglutamine (thr-MDP); N-acetyl-nor-muramyl-L-alanyl-D-isoglutamine (CGP 11637, referred to as nor-MDP); N-acetylmuramyl-L-alanyl-D-isoglutaminyl-L-alanine-2-(1′-2′-dipalmitoyl-sn-glycero-3-hydroxyphos-phoryloxy)-ethylamine (CGP 19835A, referred to a MTP-PE); RIBI, which contains three components from bacteria; monophosphoryl lipid A; trehalose dimycoloate; cell wall skeleton (MPL+TDM+CWS) in a 2% squalene/Tween 80 emulsion; and cholera toxin. Others which may be used are non-toxic derivatives of cholera toxin, including its B subunit, and/or conjugates or genetically engineered fusions of the  H. pylori  polypeptide with cholera toxin or its B subunit, procholeragenoid, fungal polysaccharides, including schizophyllan, muramyl dipeptide, muramyl dipeptide derivatives, phorbol esters, labile toxin of  E. coli , non- H. pylori  bacterial lysates, block polymers or saponins.  
     [0379] In another embodiment, the vaccine formulation includes, as a pharmaceutically acceptable carrier, a delivery system. Suitable delivery systems for use in the vaccine formulations of the invention include biodegradable microcapsules or immuno-stimulating complexes (ISCOMs), cochleates, or liposomes, genetically engineered attenuated live vectors such as viruses or bacteria, and recombinant (chimeric) virus-like particles, e.g., bluetongue. In another embodiment of the invention, the vaccine formulation includes both a delivery system and an adjuvant.  
     [0380] Delivery systems in humans may include enteric release capsules protecting the antigen from the acidic environment of the stomach, and including  H. pylori  polypeptide in an insoluble form as fusion proteins. Suitable carriers for the vaccines of the invention are enteric coated capsules and polylactide-glycolide microspheres. Suitable diluents are 0.2 N NaHCO3 and/or saline.  
     [0381] Vaccines of the invention can be administered as a primary prophylactic agent in adults or in children, as a secondary prevention, after successful eradication of  H. pylori  in an infected host, or as a therapeutic agent in the aim to induce an immune response in a susceptible host to prevent infection by  H. pylori . The vaccines of the invention are administered in amounts readily determined by persons of ordinary skill in the art. Thus, for adults a suitable dosage will be in the range of 10 μg to 10 g, preferably 10 μg to 100 mg, for example 50 μg to 50 mg. A suitable dosage for adults will also be in the range of 5 μg to 500 mg. Similar dosage ranges will be applicable for children.  
     [0382] The amount of adjuvant employed will depend on the type of adjuvant used. For example, when the mucosal adjuvant is cholera toxin, it is suitably used in an amount of 5 μg to 50 μg, for example 10 μg to 35 μg. When used in the form of microcapsules, the amount used will depend on the amount employed in the matrix of the microcapsule to achieve the desired dosage. The determination of this amount is within the skill of a person of ordinary skill in the art.  
     [0383] Those skilled in the art will recognize that the optimal dose may be more or less depending upon the patient&#39;s body weight, disease, the route of administration, and other factors. Those skilled in the art will also recognize that appropriate dosage levels can be obtained based on results with known oral vaccines such as, for example, a vaccine based on an  E. coli  lysate (6 mg dose daily up to total of 540 mg) and with an enterotoxigenic  E. coli  purified antigen (4 doses of 1 mg) (Schulman et al.,  J. Urol.  150:917-921 (1993)); Boedecker et al.,  American Gastroenterological Assoc.  999:A-222 (1993)). The number of doses will depend upon the disease, the formulation, and efficacy data from clinical trials. Without intending any limitation as to the course of treatment, the treatment can be administered over 3 to 8 doses for a primary immunization schedule over 1 month (Boedeker,  American Gastroenterological Assoc.  888:A-222 (1993)).  
     [0384] In a preferred embodiment, a vaccine composition of the invention can be based on a killed whole  E coli  preparation with an immunogenic fragment of an  H. pylori  protein of the invention expressed on its surface or it can be based on an  E. coli  lysate, wherein the killed  E. coli  acts as a carrier or an adjuvant.  
     [0385] It will be apparent to those skilled in the art that some of the vaccine 4 compositions of the invention are useful only for preventing  H. pylori  infection, some are useful only for treating  H. pylori  infection, and some are useful for both preventing and treating  H. pylori  infection. In a preferred embodiment, the vaccine composition of the invention provides protection against  H. pylori  infection by stimulating humoral and/or cell-mediated immunity against  H. pylori . It should be understood that amelioration of any of the symptoms of  H. pylori  infection is a desirable clinical goal, including a lessening of the dosage of medication used to treat  H. pylori -caused disease, or an increase in the production of antibodies in the serum or mucous of patients.  
     [0386] VII. Antibodies Reactive With  H. pylori  Polypeptides  
     [0387] The invention also includes antibodies specifically reactive with the subject  H. pylori  polypeptide. Anti-protein/anti-peptide antisera or monoclonal antibodies can be made by standard protocols (See, for example,  Antibodies: A Laboratory Manual  ed. by Harlow and Lane (Cold Spring Harbor Press: 1988)). A mammal such as a mouse, a hamster or rabbit can be immunized with an immunogenic form of the peptide. Techniques for conferring immunogenicity on a protein or peptide include conjugation to carriers or other techniques well known in the art. An immunogenic portion of the subject  H. pylori  polypeptide can be administered in the presence of adjuvant. The progress of immunization can be monitored by detection of antibody titers in plasma or serum. Standard ELISA or other immunoassays can be used with the immunogen as antigen to assess the levels of antibodies.  
     [0388] In a preferred embodiment, the subject antibodies are immunospecific for antigenic determinants of the  H. pylori  polypeptides of the invention, e.g. antigenic determinants of a polypeptide of the invention contained in the Sequence Listing, or a closely related human or non-human mammalian homolog (e.g., 90% homologous, more preferably at least 95% homologous). In yet a further preferred embodiment of the invention, the anti- H. pylori  antibodies do not substantially cross react (i.e., react specifically) with a protein which is for example, less than 80% percent homologous to a sequence of the invention contained in the Sequence Listing. By “not substantially cross react”, it is meant that the antibody has a binding affinity for a non-homologous protein which is less than 10 percent, more preferably less than 5 percent, and even more preferably less than 1 percent, of the binding affinity for a protein of the invention contained in the Sequence Listing. In a most preferred embodiment, there is no crossreactivity between bacterial and mammalian antigens.  
     [0389] The term antibody as used herein is intended to include fragments thereof which are also specifically reactive with  H. pylori  polypeptides. Antibodies can be fragmented using conventional techniques and the fragments screened for utility in the same manner as described above for whole antibodies. For example, F(ab′) 2  fragments can be generated by treating antibody with pepsin. The resulting F(ab′) 2  fragment can be treated to reduce disulfide bridges to produce Fab′ fragments. The antibody of the invention is further intended to include bispecific and chimeric molecules having an anti- H. pylori  portion.  
     [0390] Both monoclonal and polyclonal antibodies (Ab) directed against  H. pylori  polypeptides or  H. pylori  polypeptide variants, and antibody fragments such as Fab′ and F(ab′) 2 , can be used to block the action of  H. pylori  polypeptide and allow the study of the role of a particular  H. pylori  polypeptide of the invention in aberrant or unwanted intracellular signaling, as well as the normal cellular function of the  H. pylori  and by microinjection of anti- H. pylori  polypeptide antibodies of the present invention.  
     [0391] Antibodies which specifically bind  H. pylori  epitopes can also be used in immunohistochemical staining of tissue samples in order to evaluate the abundance and pattern of expression of  H. pylori  antigens. Anti  H. pylori  polypeptide antibodies can be used diagnostically in immuno-precipitation and immuno-blotting to detect and evaluate  H. pylori  levels in tissue or bodily fluid as part of a clinical testing procedure. Likewise, the ability to monitor  H. pylori  polypeptide levels in an individual can allow determination of the efficacy of a given treatment regimen for an individual afflicted with such a disorder. The level of an  H. pylori  polypeptide can be measured in cells found in bodily fluid, such as in urine samples or can be measured in tissue, such as produced by gastric biopsy. Diagnostic assays using anti- H. pylori  antibodies can include, for example immunoassays designed to aid in early diagnosis of  H. pylori  infections. The present invention can also be used as a method of detecting antibodies contained in samples from individuals infected by this bacterium using specific  H. pylori  antigens.  
     [0392] Another application of anti- H. pylori  polypeptide antibodies of the invention is in the immunological screening of cDNA libraries constructed in expression vectors such as λgt11, λgt18-23, λZAP, and λORF8. Messenger libraries of this type, having coding sequences inserted in the correct reading frame and orientation, can produce fusion proteins. For instance, λgt11 will produce fusion proteins whose amino termini consist of β-galactosidase amino acid sequences and whose carboxy termini consist of a foreign polypeptide. Antigenic epitopes of a subject  H. pylori  polypeptide can then be detected with antibodies, as, for example, reacting nitrocellulose filters lifted from infected plates with anti- H. pylori  polypeptide antibodies. Phage, scored by this assay, can then be isolated from the infected plate. Thus, the presence of  H. pylori  gene homologs can be detected and cloned from other species, and alternate isoforms (including splicing variants) can be detected and cloned.  
     [0393] VIII. Kits Containing Nucleic Acids, Polypeptides or Antibodies of the Invention  
     [0394] The nucleic acid, polypeptides and antibodies of the invention can be combined with other reagents and articles to form kits. Kits for diagnostic purposes typically comprise the nucleic acid, polypeptides or antibodies in vials or other suitable vessels. Kits typically comprise other reagents for performing hybridization reactions, polymerase chain reactions (PCR), or for reconstitution of lyophilized components, such as aqueous media, salts, buffers, and the like. Kits may also comprise reagents for sample processing such as detergents, chaotropic salts and the like. Kits may also comprise immobilization means such as particles, supports, wells, dipsticks and the like. Kits may also comprise labeling means such as dyes, developing reagents, radioisotopes, fluorescent agents, luminescent or chemiluminescent agents, enzymes, intercalating agents and the like. With the nucleic acid and amino acid sequence information provided herein, individuals skilled in art can readily assemble kits to serve their particular purpose. Kits further can include instructions for use.  
     [0395] IX. Drug Screening Assays Using  H. pylori  Polypeptides  
     [0396] By making available purified and recombinant  H. pylori  polypeptides, the present invention provides assays which can be used to screen for drugs which are either agonists or antagonists of the normal cellular function, in this case, of the subject  H. pylori  polypeptides, or of their role in intracellular signaling. Such inhibitors or potentiators may be useful as new therapeutic agents to combat  H. pylori  infections in humans. A variety of assay formats will suffice and, in light of the present inventions, will be comprehended by the skilled artisan.  
     [0397] In many drug screening programs which test libraries of compounds and natural extracts, high throughput assays are desirable in order to maximize the number of compounds surveyed in a given period of time. Assays which are performed in cell-free systems, such as may be derived with purified or semi-purified proteins, are often preferred as “primary” screens in that they can be generated to permit rapid development and relatively easy detection of an alteration in a molecular target which is mediated by a test compound. Moreover, the effects of cellular toxicity and/or bioavailability of the test compound can be generally ignored in the in vitro system, the assay instead being focused primarily on the effect of the drug on the molecular target as may be manifest in an alteration of binding affinity with other proteins or change in enzymatic properties of the molecular target. Accordingly, in an exemplary screening assay of the present invention, the compound of interest is contacted with an isolated and purified  H. pylori  polypeptide.  
     [0398] Screening assays can be constructed in vitro with a purified  H. pylori  polypeptide or fragment thereof, such as an  H. pylori  polypeptide having enzymatic activity, such that the activity of the polypeptide produces a detectable reaction product. The efficacy of the compound can be assessed by generating dose response curves from data obtained using various concentrations of the test compound. Moreover, a control assay can also be performed to provide a baseline for comparison. Suitable products include those with distinctive absorption, fluorescence, or chemi-luminescence properties, for example, because detection may be easily automated. A variety of synthetic or naturally occurring compounds can be tested in the assay to identify those which inhibit or potentiate the activity of the  H. pylori  polypeptide. Some of these active compounds may directly, or with chemical alterations to promote membrane permeability or solubility, also inhibit or potentiate the same activity (e.g., enzymatic activity) in whole, live  H. pylori  cells.  
     [0399] This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references and published patent applications cited throughout this application are hereby incorporated by reference.  
     EXEMPLIFICATION  
     [0400] I. Cloning and Sequencing of  H. pylori  DNA  
     [0401] H. pylori  chromosomal DNA was isolated according to a basic DNA protocol outlined in Schleif R. F. and Wensink P. C.,  Practical Methods in Molecular Biology , p.98, Springer-Verlag, N.Y., 1981, with minor modifications. Briefly, cells were pelleted, resuspended in TE (10 mM Tris, 1 mM EDTA, pH 7.6) and GES lysis buffer (5.1 M guanidium thiocyanate, 0.1 M EDTA, pH 8.0, 0.5% N-laurylsarcosine) was added. Suspension was chilled and ammonium acetate (NH 4 Ac) was added to final concentration of 2.0 M. DNA was extracted, first with chloroform, then with phenol-chloroform, and reextracted with chloroform. DNA was precipitated with isopropanol, washed twice with 70% EtOH, dried and resuspended in TE.  
     [0402] Following isolation whole genomic  H. pylori  DNA was nebulized (Bodenteich et al.,  Automated DNA Sequencing and Analysis  (J. C. Venter, ed.), Academic Press, 1994) to a median size of 2000 bp. After nebulization, the DNA was concentrated and separated on a standard 1% agarose gel. Several fractions, corresponding to approximate sizes 900-1300 bp, 1300-1700 bp, 1700-2200 bp, 2200-2700 bp, were excised from the gel and purified by the GeneClean procedure (Bio101, Inc.).  
     [0403] The purified DNA fragments were then blunt-ended using T4 DNA polymerase. The healed DNA was then ligated to unique BstXI-linker adapters in 100-1000 fold molar excess. These linkers are complimentary to the BstXI-cut pMPX vectors, while the overhang is not self-complimentary. Therefore, the linkers will not concatemerize nor will the cut-vector religate itself easily. The linker-adopted inserts were separated from the unincorporated linkers on a 1% agarose gel and purified using GeneClean. The linker-adopted inserts were then ligated to each of the 20 pMPX vectors to construct a series of “shotgun” subclone libraries. The vectors contain an out-of-frame lacZ gene at the cloning site which becomes in-frame in the event that an adapter-dimer is cloned, allowing these to be avoided by their blue-color.  
     [0404] All subsequent steps were based on the multiplex DNA sequencing protocols outlined in Church G. M. and Kieffer-Higgins S.,  Science  240:185-188, 1988. Only major modifications to the protocols are highlighted. Briefly, each of the 20 vectors was then transformed into DH5α competent cells (Gibco/BRL, DH5α transformation protocol). The libraries were assessed by plating onto antibiotic plates containing ampicillin, methicillin and IPTG/Xgal. The plates were incubated overnight at 37° C. Successful transformants were then used for plating of clones and pooling into the multiplex pools. The clones were picked and pooled into 40 ml growth medium cultures. The cultures were grown overnight at 37° C. DNA was purified using the Qiagen Midi-prep kits and Tip-100 columns (Qiagen, Inc.). In this manner, 100 μg of DNA was obtained per pool. Fifteen 96-well plates of DNA were generated to obtain a 5-10 fold sequence redundancy assuming 250-300 base average read-lengths.  
     [0405] These purified DNA samples were then sequenced using the multiplex DNA sequencing based on chemical degradation methods (Church G. M. and Kieffer-Higgins S.,  Science  240:185-188, 1988) or by Sequthrem (Epicenter Technologies) dideoxy sequencing protocols. The sequencing reactions were electrophoresed and transferred onto nylon membranes by direct transfer electrophoresis from 40 cm gels (Richterich P. and Church G. M.,  Methods in Enzymology  218:187-222, 1993) or by electroblotting (Church, supra). 24 samples were run per gel. 45 successful membranes were produced by chemical sequencing and 8 were produced by dideoxy sequencing. The DNA was covalently bound to the membranes by exposure to ultraviolet light, and hybridized with labeled oligonucleotides complimentary to tag sequences on the vectors (Church, supra). The membranes were washed to rinse off non-specifically bound probe, and exposed to X-ray film to visualize individual sequence ladders. After autoradiography, the hybridized probe was removed by incubation at 65° C., and the hybridization cycle repeated with another tag sequence until the membrane had been probed 38 times for chemical sequencing membranes and 10 times for the dideoxy sequencing membranes. Thus, each gel produced a large number of films, each containing new sequencing information. Whenever a new blot was processed, it was initially probed for an internal standard sequence added to each of the pools.  
     [0406] Digital images of the films were generated using a laser-scanning densitometer (Molecular Dynamics, Sunnyvale, Calif.). The digitized images were processed on computer workstations (VaxStation 4000&#39;s) using the program REPLICA™ (Church et al.,  Automated DNA Sequencing and Analysis  (J. C. Venter, ed.), Academic Press, 1994). Image processing included lane straightening, contrast adjustment to smooth out intensity differences, and resolution enhancement by iterative gaussian deconvolution. The sequences were then automatically picked in REPLICA™ and displayed for interactive proofreading before being stored in a project database. The proofreading was accomplished by a quick visual scan of the film image followed by mouse clicks on the bands of the displayed image to modify the base calls. Many of the sequence errors could be detected and corrected because multiple sequence reads covering the same portion of the genomic DNA provide adequate sequence redundancy for editing. Each sequence automatically received an identification number (corresponding to microtiter plate, probe information, and lane set number). This number serves as a permanent identifier of the sequence so it is always possible to identify the original of any particular sequence without recourse to a specialized database.  
     [0407] Routine assembly of  H. pylori  sequences was done using the program FALCON (Church, Church et al.,  Automated DNA Sequenicng and Analysis  (J. C. Venter, ed.), Academic Press, 1994). This program has proven to be fast and reliable for most sequences. The assembled contigs were displayed using a modified version of GelAssemble, developed by the Genetics Computer Group (GCG) (Devereux et al.,  Nucleic Acid Res.  12:387-95, 1984) that interacts with REPLICA™. This provided for an integrated editor that allows multiple sequence gel images to be instantaneously called up from the REPLICA™ database and displayed to allow rapid scanning of contigs and proofreading of gel traces where discrepancies occurred between different sequence reads in the assembly.  
     [0408] II. Identification, Cloning and Expression of recombinant  H. pylori  DNA Sequences  
     [0409] To facilitate the cloning, expression and purification of membrane and secreted proteins from  H. pylori  a powerful gene expression system, the pET System (Novagen), for cloning and expression of recombinant proteins in  E. coli , was selected. Also, a DNA sequence encoding a peptide tag, the His-Tag, was fused to the 3′ end of DNA sequences of interest in order to facilitate purification of the recombinant protein products. The 3′ end was selected for fusion in order to avoid alteration of any 5′ terminal signal sequence. The exception to the above was ppiB, a gene cloned for use as a control in the expression studies. In this study, the sequence for  H. pylori  ppiB contains a DNA sequence encoding a His-Tag fused to the 5′ end of the full length gene, because the protein product of this gene does not contain a signal sequence and is expressed as a cytosolic protein.  
     [0410] PCR Amplification and Cloning of DNA Sequences Containing ORF&#39;s for Membrane and Secreted Proteins from the J99 Strain of  Helicobacter pylori.    
     [0411] Sequences chosen (from the list of the DNA sequences of the invention) for cloning from the J99 strain of  H. pylori  were prepared for amplification cloning by polymerase chain reaction (PCR). Synthetic oligonucleotide primers (Table 3) specific for the 5′ and 3′ ends of open reading frames (ORFs) were designed and purchased (GibcoBRL Life Technologies, Gaithersburg, Md., USA). All forward primers (specific for the 5′ end of the sequence) were designed to include an NcoI cloning site at the extreme 5′ terminus, except for HpSeq. 4821082 (SEQ ID NO: 9730) where NdeI was used. These primers were designed to permit initiation of protein translation at a methionine residue followed by a valine residue and the coding sequence for the remainder of the native  H. pylori  DNA sequence. An exception is  H. pylori  sequence 4821082 (SEQ ID NO: 9730) where the initiator methionine is immediately followed by the remainder of the native  H. pylori  DNA sequence. All reverse primers (specific for the 3′ end of any  H. pylori  ORF) included a EcoRI site at the extreme 5′ terminus to permit cloning of each  H. pylori  sequence into the reading frame of the pET-28b. The pET-28b vector provides sequence encoding an additional 20 carboxy-terminal amino acids (only 19 amino acids in HpSeq. 26380318 (SEQ ID NO: 4851) and HpSeq.14640637 (SEQ ID NO: 8015) including six histidine residues (at the extreme C-terminus), which comprise the His-Tag. An exception to the above, as noted earlier, is the vector construction for the ppiB gene. A synthetic oligonucleotide primer specific for the 5′ end of ppiB gene encoded a BamHI site at its extreme 5′ terminus and the primer for the 3′ end of the ppiB gene encoded a XhoI site at its extreme 5′ terminus.  
                   TABLE 3                          Oligonucleotide primers used for PCR amplification of H pylon DNA se-           quences                             Forward primer 5′ to 3′   Reverse Primer 5′ to 3′                                         Outer membrane                   Proteins       Protein 16225006   5′-TATACCATGGTGGG   5′-       (aa SEQ ID NO: 9642)   CGCTAA-3′(SEQ ID   ATGAATTCGAGTAAG       (nt SEQ ID NO: 9530)   NO:9749)   GATTTTTG-3′ (SEQ ID               NO:9750)       Protein 26054702   5′-   5′-       (aa SEQ ID NO: 9450)   TTAACCATGGTGAAA   TAGAATTCGCATAAC       (nt SEQ ID NO: 4688)   AGCGATA-3′ (SEQ ID   GATCAATC-3′ (SEQ ID           NO:9751)   NO:9752)       Protein 7116626   5′-   5′-       (aa SEQ ID NO: 9048)   ATATCCATGGTGAGT   ATGAATTCAATTTTT       (nt SEQ ID NO: 4286)   TTGATGA-3′ (SEQ ID   TATTTTGCCA-3′ (SEQ           NO:9753)   ID NO:9754)       Protein 29479681   5′-   5′-       (aa SEQ ID NO: 5379)   AATTCCATGGTGGGG   ATGAATTCTCGATAG       (nt SEQ ID NO: 617)   GCTATG-3′ (SEQ ID   CCAAAATC-3′ (SEQ ID           NO:9755)   NO:9756)       Protein 14640637   5′-   5′-       (aa SEQ ID NO: 8015)   AATTCCATGGTGCAT   AAGAATTCTCTAGCA       (nt SEQ ID NO: 3253)   AACTTCCATT-3′ (SEQ   TCCAAATGGA-3′ (SEQ           ID NO:9757)   ID NO:9758)       Periplasmic/Secreted               Proteins               Protein 30100332   5′-ATTTCCATGGTCATG   5′-       (aa SEQ ID NO: 5933)   TCTCATATT-3′ (SEQ ID   ATGAATTCCATCTTT       (nt SEQ ID NO: 1171)   NO:9759)   TATTCCAC-3′ (SEQ ID               NO:9760)       Protein 4721061   5′-AACCATGGTGATTT   5′-       (aa SEQ ID NO: 5181)   TAAGCATTGAAAG-3′   AAGAATTCCACTCA       (nt SEQ ID NO: 419)   (SEQ ID NO:9761)   AAATTTTTTAACAG-3′               (SEQ ID NO:9762)       Other Surface Proteins               Protein 4821082   5′-GATCATCCATATGTT   5′-       (aa SEQ ID NO: 9730)   ATCTTCTAAT-3′ (SEQ   TCAATCAACCATTT       (nt SEQ ID NO: 9618   ID NO:9763)   TAACCCTG-3′ (SEQ ID               NO:9764)       Protein 978477   5′-TATACCATGGTGAA   5′-       (aa SEQ ID NO: 5231)   ATTTTTTCTTTTA-3′   AGAATTCAATTGCG       (nt SEQ ID NO: 469)   (SEQ ID NO:9765)   TCTTGTAAAAG-3′               (SEQ ID NO:9766)       Inner Membrane               Protein               Protein 26380318   5′-TATACCATGGTGAT   5′-ATGAATTCCCACTT       (aa SEQ ID NO: 4851)   GGACAAACTC-3′ (SEQ   GGGGCGATA-3′ (SEQ       (nt SEQ ID NO: 89)   ID NO:9767)   ID NO:9768)       Cytoplasmic Protein               ppi   5′-TTATGGATCCAAAC   5′-TATCTCGAGTTATA       (aa SEQ ID NO: 7674)   CAATTAAAACT-3′ (SEQ   GAGAAGGGC-3′ (SEQ       (nt SEQ ID NO: 2912)   ID NO:9769)   ID NO:9770)                  
 
     [0412] Genomic DNA prepared from the J99 strain of  H. pylori  (ATCC #55679; deposited by Genome Therapeutics Corporation, 100 Beaver Street, Waltham, Mass. 02154) was used as the source of template DNA for PCR amplification reactions (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). To amplify a DNA sequence containing an  H. pylori  ORF, genomic DNA (50 nanograms) was introduced into a reaction vial containing 2 mM MgCl 2 , 1 micromolar synthetic oligonucleotide primers (forward and reverse primers) complementary to and flanking a defined  H. pylori  ORF, 0.2 mM of each deoxynucleotide triphosphate; dATP, dGTP, dCTP, dTTP and 2.5 units of heat stable DNA polymerase (Amplitaq, Roche Molecular Systems, Inc., Branchburg, N.J., USA) in a final volume of 100 microliters. The following thermal cycling conditions were used to obtain amplified DNA products for each ORF using a Perkin Elmer Cetus/GeneAmp PCR System 9600 thermal cycler:  
     [0413] Protein 26054702 (SEQ ID NO: 9450), Protein 7116626 (SEQ ID NO: 9048), Protein 29479681 (SEQ ID NO: 5379), Protein 30100332 (SEQ ID NO: 5933), and Protein 4821082(SEQ ID NO: 9730);  
     [0414] Denaturation at 94° C. for 2 min,  
     [0415] 2 cycles at 94° C. for 15 sec, 30° C. for 15 sec and 72° C. for 1.5 min  
     [0416] 23 cycles at 94° C. for 15 sec, 55° C. for 15 sec and 72° C. for 1.5 min  
     [0417] Reactions were concluded at 72° C. for 6 minutes.  
     [0418] Protein 16225006 (SEQ ID NO: 9642);  
     [0419] Denaturation at 94° C. for 2 min,  
     [0420] 25 cycles at 95° C. for 15 sec, 55° C. for 15 sec and 72° C. for 1.5 min  
     [0421] Reaction was concluded at 72° C. for 6 minutes.  
     [0422] Protein 4721061 (SEQ ID NO: 5181);  
     [0423] Denaturation at 94° C. for 2 min,  
     [0424] 2 cycles at 94° C. for 15 sec, 36° C. for 15 sec and 72° C. for 1.5 min  
     [0425] 23 cycles at 94° C. for 15 sec, 60° C. for 15 sec and 72° C. for 1.5 min  
     [0426] Reactions were concluded at 72° C. for 6 minutes.  
     [0427] Protein 26380318(SEQ ID NO: 4851);  
     [0428] Denaturation at 94° C. for 2 min,  
     [0429] 2 cycles at 94° C. for 15 sec, 38° C. for 15 sec and 72° C. for 1.5 min  
     [0430] 23 cycles at 94° C. for 15 sec, 62° C. for 15 sec and 72° C. for 1.5 min  
     [0431] Reactions were concluded at 72° C. for 6 minutes.  
     [0432] Protein 14640637 (SEQ ID NO: 8015);  
     [0433] Denaturation at 94° C. for 2 min,  
     [0434] 2 cycles at 94° C. for 15 sec, 33° C. for 15 sec and 72° C. for 1.5 min  
     [0435] 30 cycles at 94° C. for 15 sec, 55° C. for 15 sec and 72° C. for 1.5 min  
     [0436] Reactions were concluded at 72° C. for 6 minutes.  
     [0437] Conditions for amplification of  H. pylori  ppiB (SEQ ID NO: 7674);  
     [0438] Denaturation at 94° C. for 2 min,  
     [0439] 2 cycles at 94° C. for 15 sec, 32° C. for 15 sec and 72° C. for 1.5 min  
     [0440] 25 cycles at 94° C. for 15 sec, 56° C. for 15 sec and 72° C. for 1.5 min  
     [0441] Reactions were concluded at 72° C. for 6 minutes  
     [0442] Upon completion of thermal cycling reactions, each sample of amplified DNA was washed and purified using the Qiaquick Spin PCR purification kit (Qiagen, Gaithersburg, Md., USA). All amplified DNA samples were subjected to digestion with the restriction endonucleases, NcoI and EcoRI (New England BioLabs, Beverly, Mass., USA), or in the case of HpSeq. 4821082 (SEQ ID NO: 9730), with NdeI and EcoRI (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). DNA samples were then subjected to electrophoresis on 1.0% NuSeive (FMC BioProducts, Rockland, Me. USA) agarose gels. DNA was visualized by exposure to ethidium bromide and long wave uv irradiation. DNA contained in slices isolated from the agarose gel was purified using the Bio 101 GeneClean Kit protocol (Bio 101 Vista, Calif., USA).  
     [0443] Cloning of  H. pylori  DNA Sequences into the pET-28b Prokaryotic Expression Vector.  
     [0444] The pET-28b vector-was prepared for cloning by digestion with NcoI and EcoRI, or in the case of  H. pylori  protein 4821082 (SEQ ID NO: 9730) with NdeI and EcoRI (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). In the case of cloning ppiB, the pET-28a vector, which encodes a His-Tag that can be fused to the 5′ end of an inserted gene, was used and the cloning site prepared for cloning with the ppiB gene by digestion with BamHI and XhoI restriction endonucleases.  
     [0445] Following digestion, DNA inserts were cloned (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994) into the previously digested pET-28b expression vector, except for the amplified insert for ppiB, which was cloned into the pET-28a expression vector. Products of the ligation reaction were then used to transform the BL21 strain of  E. coli  (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994) as described below.  
     [0446] Transformation of Competent Bacteria with Recombinant Plasmids  
     [0447] Competent bacteria,  E coli  strain BL21 or  E. coli  strain BL21 (DE3), were transformed with recombinant pET expression plasmids carrying the cloned  H. pylori  sequences according to standard methods (Current Protocols in Molecular, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). Briefly, 1 microliter of ligation reaction was mixed with 50 microliters of electrocompetent cells and subjected to a high voltage pulse, after which, samples were incubated in 0.45 milliliters SOC medium (0.5% yeast extract, 2.0% tryptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4 and 20, mM glucose) at 37° C. with shaking for 1 hour. Samples were then spread on LB agar plates containing 25 microgram/ml kanamycin sulfate for growth overnight. Transformed colonies of BL21 were then picked and analyzed to evaluate cloned inserts as described below.  
     [0448] Identification of Recombinant pET Expression Plasmids Carrying  H. pylori  Sequences  
     [0449] Individual BL21 clones transformed with recombinant pET-28b- H. pylori  ORFs were analyzed by PCR amplification of the cloned inserts using the same forward and reverse primers, specific for each  H. pylori  sequence, that were used in the original PCR amplification cloning reactions. Successful amplification verified the integration of the  H. pylori  sequences in the expression vector (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994).  
     [0450] Isolation and Preparation of Plasmid DNA from BL21 Transformants  
     [0451] Individual clones of recombinant pET-28b vectors carrying properly cloned  H. pylori  ORFs were picked and incubated in 5 mls of LB broth plus 25 microgram/ml kanamycin sulfate overnight. The following day plasmid DNA was isolated and purified using the Qiagen plasmid purification protocol (Qiagen Inc., Chatsworth, Calif., USA).  
     [0452] Expression of Recombinant  H. pylori  Sequences in  E. coli    
     [0453] The pET vector can be propagated in any  E. coli  K-12 strain e.g. HMS 174, HB 101, JM 109, DH5, etc. for the purpose of cloning or plasmid preparation. Hosts for expression include  E. coli  strains containing a chromosomal copy of the gene for T7 RNA polymerase. These hosts are lysogens of bacteriophage DE3, a lambda derivative that carries the lacI gene, the lacUV5 promoter and the gene for T7 RNA polymerase. T7 RNA polymerase is induced by addition of isopropyl-B-D-thiogalactoside (IPTG), and the T7 RNA polymerase transcribes any target plasmid, such as pET-28b, carrying a T7 promoter and a gene of interest. Strains used include: BL21(DE3) (Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W. (1990) Meth. Enzymol. 185, 60-89).  
     [0454] To express recombinant  H. pylori  sequences, 50 nanograms of plasmid DNA isolated as described above was used to transform competent BL21 (DE3) bacteria as described above (provided by Novagen as part of the pET expression system kit). The lacZ gene (beta-galactosidase) was expressed in the pET-System as described for the  H. pylori  recombinant constructions. Transformed cells were cultured in SOC medium for 1 hour, and the culture was then plated on LB plates containing 25 micrograms/ml kanamycin sulfate. The following day, bacterial colonies were pooled and grown in LB medium containing kanamycin sulfate (25 micrograms/ml) to an optical density at 600 nM of 0.5 to 1.0 O.D. units, at which point, 1 millimolar IPTG was added to the culture for 3 hours to induce gene expression of the  H. pylori  recombinant DNA constructions.  
     [0455] After induction of gene expression with IPTG, bacteria were pelleted by centrifugation in a Sorvall RC-3B centrifuge at 3500×g for 15 minutes at 4° C. Pellets were resuspended in 50 milliliters of cold 10 mM Tris-HCl, pH 8.0, 0.1 M NaCl and 0.1 mM EDTA (STE buffer). Cells were then centrifuged at 2000×g for 20 min at 4° C. Wet pellets were weighed and frozen at −80° C. until ready for protein purification.  
     [0456] III. Purification of Recombinant Proteins from  E. coli    
     [0457] Analytical Methods  
     [0458] The concentrations of purified protein preparations were quantified spectrophotometrically using absorbance coefficients calculated from amino acid content (Perkins, S. J. 1986 Eur. J. Biochem. 157, 169-180). Protein concentrations were also measured by the method of Bradford, M. M. (1976) Anal. Biochem. 72, 248-254, and Lowry, O. H., Rosebrough, N., Farr, A. L. &amp; Randall, R. J. (1951) J. Biol. Chem. 193, pages 265-275, using bovine serum albumin as a standard.  
     [0459] SDS-polyacrylamide gels (12% or 4.0 to 25% acrylamide gradient gels) were purchased from BioRad (Hercules, Calif., USA), and stained with Coomassie blue. Molecular weight markers included rabbit skeletal muscle myosin (200 kDa),  E. coli  (-galactosidase (116 kDa), rabbit muscle phosphorylase B (97.4 kDa), bovine serum albumin (66.2 kDa), ovalbumin (45 kDa), bovine carbonic anhydrase (31 kDa), soybean trypsin inhibitor (21.5 kDa), egg white lysozyme (14.4 kDa) and bovine aprotinin (6.5 kDa).  
     [0460] 1. Purification of Soluble Proteins  
     [0461] All steps were carried out at 4° C. Frozen cells were thawed, resuspended in 5 volumes of lysis buffer (20 mM Tris, pH 7.9, 0.5 M NaCl, 5 mM imidazole with 10% glycerol, 0.1% 2-mercaptoethanol, 200 μg/ml lysozyme, 1 mM phenylmethylsulfonyl fluoride (PMSF), and 10 ug/ml each of leupeptin, aprotinin, pepstatin, L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone (TLCK), L-1-chloro-3-[4-tosylamido]-4-phenyl-2-butanone (TPCK), and soybean trypsin inhibitor, and ruptured by several passages through a small volume microfluidizer (Model M-110S, Microfluidics International Corporation, Newton, Mass.). The resultant homogenate was made 0.1% Brij 35, and centrifuged at 100,000×g for 1 hour to yield a clear supernatant (crude extract).  
     [0462] Following filtration through a 0.8 μm Supor filter (Gelman Sciences, FRG) the crude extract was loaded directly onto a Ni 2+ -nitrilotriacetate-agarose (NTA) with a 5 milliliter bed volume (Hochuli, E., Dbeli, H., and Schacheer, A. (1987) J. Chromatography 411, 177-184) pre-equilibrated in lysis buffer containing 10% glycerol, 0.1% Brij 35 and 1 mM PMSF. The column was washed with 250 ml (50 bed volumes) of lysis buffer containing 10% glycerol, 0.1% Brij 35, and was eluted with sequential steps of lysis buffer containing 10% glycerol, 0.05% Brij 35, 1 mM PMSF, and 20, 100, 200, and 500 mM imidazole in succession. Fractions were monitored by absorbance at OD 280  nm, and peak fractions were analyzed by SDS-PAGE. Fractions containing the recombinant protein eluted at 100 mM imidazole.  
     [0463] Recombinant Protein 1-, 5-0637 (SEQ ID NO: 8015) and Proteins, Beta-Galactosidase (lacZ) and Peptidyl-prolyl Cis-trans Isomerase (ppiB SEQ ID NO: 7674)  
     [0464] Fractions containing the recombinant proteins from the Ni 2+ -NTA-agarose columns were pooled and then concentrated to approximately 5 ml by centrifugal filtration (Centriprep-10, Amicon, Mass.), and loaded directly onto a 180-ml column (1.6×91 cm) of Sephacryl S-100 HR gel filtration medium equilibrated in Buffer A (10 mM Hepes, pH 7.5, 150 mM NaCl, 0.1 mM EGTA) and run in Buffer A at 18 ml/h. Fractions containing the recombinant protein were identified by absorbance at 280 nm and analyzed by SDS-PAGE. Fractions were pooled and concentrated by centrifugal filtration.  
     [0465] Recombinant Protein 7116626 (SEQ ID NO: 9048)  
     [0466] Fractions containing the recombinant protein from the Ni 2+ -NTA-agarose column were pooled and dialyzed overnight against 1 liter of dialysis buffer (10 mM MOPS, pH 6.5, 50 mM NaCl, 0.1 mM EGTA, 0.02% Brij 35 and 1 mM PMSF). In the morning, a fine white precipitate was removed by centrifugation and the resulting supernatant was loaded onto an 8 ml (8×75 mm) MonoS high performance liquid chromatography column (Pharmacia Biotechnology, Inc., Piscataway, N.J., USA) equilibrated in buffer B (10 mM MOPS, pH 6.5, 0.1 mM EGTA) containing 50 mM NaCl. The column was washed with 10 bed volumes of buffer B containing 50 mM NaCl, and developed with a 50 ml linear gradient of increasing NaCl (50 to 500 mM). Recombinant protein 7116626 eluted as a sharp peak at 300 mM NaCl.  
     [0467] 2. Purification of Insoluble Proteins from Inclusion Bodies  
     [0468] The following steps were carried out at 4° C. Cell pellets were resuspended in lysis buffer with 10% glycerol 200 μg/ml lysozyme, 5 mM EDTA, 1 mM PMSF and 0.1%-mercaptoethanol. After passage through the cell disrupter, the resulting homogenate was made 0.2% deoxycholate, stirred 10 minutes, then centrifuged at 20,000×g, for 30 min. The pellets were washed with lysis buffer containing 10% glycerol, 10 mM EDTA, 1% Triton X-100, 1 mM PMSF and 0.1%-mercaptoethanol, followed by several washes with lysis buffer containing 1 M urea, 1 mM PMSF and 0.1% 2-mercaptoethanol. The resulting white pellet was composed primarily of inclusion bodies, free of unbroken cells and membranous materials.  
     [0469] Recombinant Proteins 26054702 (SEQ ID NO: 9450), 16225006 (SEQ ID NO: 9642), 30100332 (SEQ ID NO: 5933), 4721061 (SEQ ID NO: 5181), 978977 (SEQ ID NO: 523)  
     [0470] The following steps were carried out at room temperature. Purified inclusion bodies were dissolved in 20 ml 8.0 M urea in lysis buffer with 1 mM PMSF and 0.1% 2-mercaptoethanol, and incubated at room temperature for 1 hour. Materials that did not dissolve were removed by centrifugation. The clear supernatant was filtered, then loaded onto a Ni 2+ -NTA agarose column pre-equilibrated in 8.0 M urea in Lysis Buffer. The column was washed with 250 ml (50 bed volumes) of lysis buffer containing 8 M urea, 1.0 mM PMSF and 0.1% 2-mercaptoethanol, and developed with sequential steps of lysis buffer containing 8M urea, 1 mM PMSF, 0.1% 2-mercaptoethanol and 20, 100, 200, and 500 mM imidazole in succession. Fractions were monitored by absorbance at OD 280  nm, and peak fractions were analyzed by SDS-PAGE. Fractions containing the recombinant protein eluted at 100 mM imidazole.  
     [0471] Recombinant Proteins 29479681 (SEQ ID NO: 5379), 26380318 (SEQ ID NO: 4851)  
     [0472] The pellet containing the inclusion bodies was solubilized in buffer B containing 8 M urea, 1 mM PMSF and 0.1% 2-mercaptoethanol, and incubated for 1 hour at room temperature. Insoluble materials were removed by centrifugation at 20,000×g for 30 min, and the cleared supernatant was loaded onto a 15 ml (1.6×7.5 cm) SP-Sepharose column pre-equilibrated in buffer B, 6 M urea, 1 mM PMSF, 0.1% 2-mercaptoethanol. After washing the column with 10 bed volumes, the column was developed with a linear gradient from 0 to 500 mM NaCl.  
     [0473] Dialysis and Concentration of Protein Samples  
     [0474] Urea was removed slowly from the protein samples by dialysis against Tris-buffered saline (TBS; 10 mM Tris pH 8.0, 150 mM NaCl) containing 0.5% deoxycholate (DOC) with sequential reduction in urea concentration as follows; 6M, 4M, 3M; 2M, 1M, 0.5 M and finally TBS without any urea. Each dialysis step was conducted for a minimum of 4 hours at room temperature.  
     [0475] After dialysis, samples were concentrated by pressure filtration using Amicon stirred-cells. Protein concentrations were measured using the methods of Perkins (1986 Eur. J. Biochem. 157, 169-180), Bradford ((1976) Anal. Biochem. 72, 248-254) and Lowry ((1951) J. Biol. Chem. 193, pages 265-275).  
     [0476] The recombinant proteins purified by the methods described above are summarized in Table 4 below.  
                                           TABLE 4                                      Bacterial cell       Relative   Final                   Gene   fraction used       MW on   concentra-           J99   Homolog   symbol   to purify       SDS-   tion of   Compo-       Sequence   identified   of   recombinant   Method of   PAGE   purified   sition of       Identifier   by Blast   Homolog   proteins   purification   gel   protein   buffer                         Outer Membrane Proteins                                             16225006   P28635   YEAC   Inclusion   His-Tag    18 kDa   5 mg/ml   B       (SEQ ID           bodies       NO: 9642)       26054702   P15929   flgH   Inclusion   His-Tag    37 kDa   1.18   B       (SEQ ID           bodies           mg/ml       NO: 9450)                               —   as dry                                   pellet       7116626   P26093   e(P4)   Soluble   His-Tag    29 kDa   0.8 mg/ml   A       (SEQ ID           fraction       NO: 9048)                               1.85   C                               mg/ml       29479681   P13036   fecA   Inclusions   SP-    23 kDa   2.36   B       (SEQ ID           bodies   Sepharose       mg/ml       NO: 5379)                               0.5 mg/ml   B                               —   as dry                                   pellet       14640637   P16665   TPF1   Soluble   His-Tag    17 kDa   2.4 mg/ml   A       (SEQ ID           fraction       NO: 8015)                                                     gel filtration S100 HR                     Periplasmic/Secreted Protein                                             30100332   P23847   dppA   Inclusion   His-Tag    11 kDa   2.88   B       (SEQ ID           bodies           mg/ml       NO: 5933)       4721061   P36175   GCP   Inclusion   His-Tag    38 kDa   2.8 mg/ml   B       (SEQ ID           bodies       NO: 5181)                 Other Surface Proteins                                             4821082   P08089   M   Inclusion   His-Tag    20 kDa   1.16   B       (SEQ ID       protein   bodies           mg/ml       NO: 9730)       978477   L28919   FBP54   Inclusion   SP-    44 kDa   2.56   B       (SEQ ID           bodies   Sepharose       mg/ml       NO: 5231)                               0.3 mg/ml   B                 Inner Membrane Proteins                                             26380318   P15933   fliG   Inclusion   SP-    11 kDa   22 mg/ml   B       (SEQ ID           bodies   Sepharose       NO: 4851)                 Control Proteins with His-Tag                                                 P00722   lacZ   Soluble   His-Tag   116 kDa   10 mg/ml   A                   fraction                                                     gel filtration S200 HR                                                 ppiB       ppiB   Soluble   His-Tag    21 kDa   4.4 mg/ml   A       (SEQ ID           fraction       NO: 7674)                                                     gel filtration S100 HR                                                      
 
     [0477] IV. Analysis of  H. pylori  Proteins as Vaccine Candidates  
     [0478] To analyze  H. pylori  proteins for use in the vaccine formulations of the invention, several  H. pylori  proteins were expressed, characterized immunologically and tested in animal efficacy studies as outlined below. Specifically, the immunomodulatory effects of  H. pylori  proteins were investigated in a mouse/ H. pylori  model which mimics the human  H. pylori  infection in humans. In these studies, the effect of oral immunization of selected  H. pylori  polypeptides in  H. pylori  infected mice was determined. Those skilled in the art will recognize that in the examples outlined below, the use of cholera toxin (CT) was only illustrative and is not essential for achieving oral immunization.  
     [0479] Identification, Cloning and Expression of Recombinant  Helicobacter Pylori  Sequences.  
     [0480] To facilitate the cloning, expression and purification of membrane and/or secreted proteins from  H. pylori , the pET gene expression system (Novagen), for cloning and expression of recombinant proteins in  Escherichia coli  was selected. Further, for proteins that have a signal sequence at their amino-terminal end, a DNA sequence encoding a peptide tag (His-tag) was fused to the 5′ end of the  H. pylori  DNA sequences of interest in order to facilitate purification of the recombinant protein products.  
     [0481] PCR Amplification and Cloning of DNA Sequences Containing ORFs for Membrane and Secreted Proteins from the J99 Strain of  Helicobacter Pylori.    
     [0482] The sequences selected (from the list of the DNA sequences of the invention) for cloning from  H. pylori  strain J99 were prepared for amplification cloning by the polymerase chain reaction (PCR). All of the selected sequences encode for outer membrane  H. pylori  proteins, with vac9 (SEQ ID NO:4974), vac10 (SEQ ID NO:5016), vac22 (SEQ ID NO:4967) and vac41 (SEQ ID NO:5051) sequences all sharing a terminal phenylalanine residue. Likewise, the vac32 (SEQ ID NO:5091), vac36 (SEQ ID NO:5146) and vac37 (SEQ ID NO:5139) sequences all share a terminal phenylalanine residue and a tyrosine cluster at the C-terminus. Synthetic oligonucleotide primers for each ORF of interest (Table 5) specific for the predicted mature 5′ end of the ORF and downstream (3′) of the predicted translational termination codon were designed and purchased (GibcoBRL Life Technologies, Gaithersburg, Md., USA). All forward primers (specific for the 5′ terminus of the region of ORF of interest) were designed to include a BamIII restriction site followed by a NdeI restriction site. These primers were designed to permit the initiation of protein translation at a methionine residue encoding within the NdeI restriction site sequence (in the case of producing a non His-tagged recombinant protein) or to fuse in frame with the DNA sequence encoding the His-tag (for producing His-tagged recombinant protein), followed by the coding sequence for the remainder of the native  H. pylori  DNA. All reverse oligonucleotide primers (specific for downstream (3′) of the predicted translational termination codon of the ORF) were designed to include an EcoRI restriction site at the 5′ terminus. This combination of primers would enable each ORF of interest to be cloned into pET28b (to produce a His-tagged recombinant protein) or pET30a (to produce a non His-tagged or native recombinant protein). The pET28b vector provides sequence encoding an additional 20 amino-terminal amino acids (plus the methionine in the NdeI restriction site) including a stretch of six histidine residues which makes up the His-tag.  
     [0483] Genomic DNA prepared from  H. pylori  strain J99 (ATCC 55679) was used as the source of template DNA for the PCR amplification reactions (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). To amplify a DNA sequence containing a specific  H. pylori  ORF, genomic DNA (50 nanograms) was introduced into a reaction tube containing 200 nanograms of both the forward and reverse synthetic oligonucleotide primer specific for the ORF of interest, and 45 microliters of PCR SuperMix purchased (GibcoBRL Life Technologies, Gaithersburg, Md., USA) in a total of 50 microliters. The PCR SuperMix is supplied in 1.1× concentrations and contains 22 mM Tris-HCl (pH 8.4), 55 mM KCl, 1.65 mM MgCl 2 , 220 micromolar of each dATP, dCTP, dGTP and dTTP, 22 units recombinant Taq polymerase/ml and stabilizers. The following thermal cycling conditions were used to obtain amplified DNA products for each ORF using a Perkin Elmer Cetus/Gene Amp PCR System thermal cycler.  
                   TABLE 5                          Oligonucleotide primers                             Gene   Forward primer   Reverse primer                                     vac9   CGCGGATCCATATGGCTGAAA   CCGGAATTCATCAGTATTCAA           (nt SEQ ID   AAACGCCTTTTTTTAAAACTAA   TGGGAATAAAGCC (SEQ ID       NO:212)   AAACCAC (SEQ ID NO: 9771)   NO: 9772)       (aa SEQ ID NO: 4974)               vac 10   CGCGGATCCATATGAAAGAAG   CCGGAATTCGCTTAAAAGAAA       (nt SEQ ID   AAGAAAAAGAAGAAAAAAAG   ATAGTCCCCCAAACGC (SEQ       NO:254)   ACAGAAAGG (SEQ ID NO: 9773)   ID NO: 9774)       (aa SEQ ID NO: 5016)               vac22   CGCCGGATCCATATGAAAGAG   CCGGAATTCATATAAATATCA       (nt SEQ ID   GTCATTCCACCCCTTCAACCCC   TATAGGCAGAAAAAC (SEQ ID       NO:205)   (SEQ ID NO: 9775)   NO: 9776)       (aa SEQ ID NO: 4967)               vac32   CGCGGATCCATATGGAGGCAG   CCGGAATTCGATTGATTTTGTC       (ft SEQ ID   AGCTTGATGAAAAATC (SEQ ID   AAATCTAAAATCCC (SEQ ID       NO:329)   NO: 9777)   NO: 9778)       (aa SEQ ID NO:5091)               vac36 (hop   TATTATACATATGGAAGAAGA   TAATCTCGAGTTTAGAAGGCG       B)   TGGG (SEQ ID NO: 9779)   TA (SEQ ID NO: 9780)       (nt SEQ ID               NO:384)               (aa SEQ ID NO:5146)               vac37   TTATATTCATATGGAAGACGAT   AATTCTCGAGCCTCTTTATAA       (i-hop)   GGC (SEQ ID NO: 9781)   GCC (SEQ ID NO:9782)       (nt SEQ ID               NO:377)               (aa SEQ ID NO: 5139)               vac41   CGCGGATCCATATGGTAGAAG   CCGGAATTCGGAGCCAATAGG       (nt SEQ ID   CCTTTCAAAAACACCAAAAAG   GAGCTAAAGCC (SEQ ID NO:       NO:289)   ACGG (SEQ ID NO: 9783)   9784)       (aa SEQ ID NO: 5051)                  
 
     [0484] Sequences for Vac32 (SEQ ID NO: 5091), Vac9 (SEQ ID NO: 4974) and Vac22 (SEQ ID NO: 4967)  
     [0485] Denaturation at 94° C. for 30 sec  
     [0486] 35 cycles at 94° C. for 15 sec, 55° C. for 15 sec, and 72° C. for 1.5 min  
     [0487] Reactions were concluded at 72° C. for 8 minutes  
     [0488] Sequences for Vac10 (SEQ ID NO: 5016) and Vac41 (SEQ ID NO: 5051)  
     [0489] Denaturation at 94° C. for 30 sec  
     [0490] 35 cycles at 94° C. for 15 sec, 55° C. for 15 sec, and 72° C. for 2.5 min  
     [0491] Reactions were concluded at 72° C. for 8 minutes  
     [0492] Sequences for Vac36 (SEQ ID NO: 5146) and Vac37 (SEQ ID NO: 5139)  
     [0493] Denaturation at  
     [0494] 2 cycles at 94° C. for 15 sec, 30° C. for 15 sec, and 72° C. for 1.5 min  
     [0495] 23 cycles at 94° C. for 15 sec, 55° C. for 1.5 sec, and 72° C. for 1.5 min  
     [0496] Reactions were concluded at 72° C. for 6 minutes  
     [0497] Upon completion of the thermal cycling reactions, each sample of amplified DNA was subjected to electrophoresis on 1.0% agarose gels. The DNA was visualized by exposure to ethidium bromide and long wave UV irradiation, and cut out in gel slices. DNA was purified using the Wizard PCR Preps Kit (Promega Corp., Madison, Wis., USA), and then subjected to digestion with BamHI and EcoRI (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). The digested PCR amplicon was then re-electrophoresed and purified as before.  
     [0498] Ligation of  H. Pylori  DNA Sequences into Cloning Vectors  
     [0499] The pOK12 vector (J. Vieira and J. Messing, Gene 100:189-194, 1991) was prepared for cloning for digestion with BamHI and EcoRI in the case of Vac9, 10, 22, 31 and 32, whereas the pSU21 vector (B. Bartolome et al., Gene 102:75-78, 1991) was prepared for cloning by digestion with BamHI and EcoRI in the case of Vac 41 (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). The vectors were subjected to electrophoresis on 1.0% agarose gels and purified using the Wizard PCR Preps kit (Promega Corp., Madison, Wis., USA). Following ligation of the purified, digested vector and the purified, digested amplified  H. pylori  ORF, the products of the ligation reaction were transformed into  E. coli  JM 109 competent cells according to standard methods (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). Individual bacterial colonies were screened for those containing the correct recombinant plasmids by incubating in LB broth overnight (plus 25 ug/ml kanamycin sulfate for the pOK12 based plasmids or 25 ug/ml chloramphenicol for the pSU21 based plasmids) followed by plasmid DNA preparation using the Magic Minipreps system (Promega Corp., Madison, Wis., USA), and then analyzed by restriction digestion (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994).  
     [0500] Cloning of  H. Pylori  DNA Sequences into the pET28b and pET30a Prokaryotic Expression Vectors  
     [0501] Both the pET28b and pET30a expression vectors were prepared for cloning by digestion with NdeI and EcoRI (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). The  H. pylori  DNA sequences were removed from pOK12 (Vac9, 10, 23, 31 and 32) or pSU21 (Vac41) plasmid backbones by digestion with NdeI and EcoRI (Current Protocols in Molecular Biology, John Wiley and Son, Inc., F. Ausubel et al., eds., 1994). The pET28b, pET30a and  H. pylori  DNA sequences were all electrophoresed on a 1% agarose gel and purified using the Wizard PCR Preps kit (Promega Corp., Madison Wis., USA). Following ligation of the purified, digested expression vector and the purified, digest  H. pylori  DNA sequences, the products of the ligation reaction were transformed into  E coli  JM109 competent cells (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). Individual bacterial colonies were screened for those containing the correct recombinant plasmids by preparing plasmid DNA as described above followed by analysis by restriction digestion profiles and DNA sequencing (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al, eds., 1994). These recombinant plasmids were then used to transform specific  E. coli  expression strains.  
     [0502] Transformation of Competent Bacteria with Recombinant Expression Plasmids  
     [0503] Competent bacterial strains (BL21 (DE3), BL21 (DE3)pLyS, HMS174(DE3) and HMS174(DE3)pLysS were prepared and transformed with the recombinant pET28b expression plasmids carrying the cloned  H. pylori  sequences according to standard methods (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). These expression host strains contain a chromosomal copy of the gene for T7 RNA polymerase. These hosts are lysogens of bacteriophage DE3, a lambda derivate that carries the lacI gene, the lacUV5 promoter and the gene for T7 RNA polymerase. T7 RNA polymerase expression is induced by the addition of isopropyl-β-D thiogalactoside (1PTG), and the T7 RNA polymerase then transcribes any target plasmid, such as pET28b, that carries a T7 promoter sequence and a gene of interest.  
     [0504] Expression of Recombinant  H. Pylori  Sequences in  E. Coli    
     [0505] Transformants were collected from LB agar plates containing 25 ug/ml kanamycin sulfate (ensures maintenance of the pET28b-based recombinant plasmids) and used to inoculate LB broth containing 25 ug/ml kanamycin sulfate and grown to an optical density at 600 nm of 0.5 to 1.0 OD units, at which point 1 mM 1 PTG was added to the culture for one to three hours to induce gene expression of the  H. pylori  recombinant DNA constructions. After induction of gene expression with 1PTG, bacteria were pelleted by centrifugation and resuspended in SDS-PAGE solubilization buffer and subjected to SDS-PAGE (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). Proteins were visualized by staining with Coomassie Brilliant Blue or detected by western immunoblotting using the specific anti-His tag monoclonal antibody (Clontech, Palo Alto, Calif., USA) using standard methods (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). The host strain that provided the highest level of recombinant protein production was then chosen for use in a large-scale induction in order to purify the recombinant protein. All of the following proteins listed were expressed recombinantly and the strain giving the highest level of expression listed: BL21(DE3) (vac31, vac26, vac37; BL21(DE3) pLysS (vac 9, 32); HMS174(DE3) (vac10).  
     [0506] Purification of Recombinant Proteins and Generation of Specific Antiserum  
     [0507] Large scale cultures were inoculated and grown as above, and induced with 1 mM 1 PTG for 3 hours. After induction, bacteria were pelleted by centrifugation in a Sorvall centrifuge at 3500×g for 15 min at 4° C. All of the expressed recombinant proteins were present in the insoluble inclusion body fraction. Inclusion bodies were purified according to standard protocols (Antibodies, Cold Spring Harbor Laboratory Press, E. Harlow and D. Lane, eds., 1988). The recombinant protein produced by vac32 was solubilized in 8M urea and partially purified by nickel chromatography (REF here). Denatured recombinant proteins were purified by electrophoresis on SDS-PAGE gels, and after visualization with Coomassie Brilliant Blue, the protein was excised from the gel and the gel slices homogenized. This material was used to raise specific polyclonal antibodies in mice or rabbits according to standard protocols (Antibodies, Cold Spring Harbor Laboratory Press, E. Harlow and D. Lane, eds., 1988).  
     [0508] Immunological Characterization of Recombinant Proteins  
     [0509] In all cases where antibody was attempted to be raised, high titre antisera was generated, confirming the immunogenicity of the recombinant proteins. Further, these specific antisera were used to analyze whether the protein encoded by the cloned gene was expressed in  H. pylori . Western immunoblot analysis using standard protocols (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994) confirmed that the  H. pylori  strain J99 did express proteins of the expected molecular weight that reacted with the vac10, vac32, vac31, vac36 antiserum. The specific antiserum was also used to determine the level of antigenic conservation between a large number of  H. pylori  isolates that had been obtained from distinct geographical sites around the world, and from all types of clinical manifestations, including gastritis, duodenal ulcer, gastric ulcer and gastric cancer. It was found that every strain produced a protein that reacted specifically with each antiserum.  
     [0510] Further,  H. pylori  cells from strains J99, 17874, AH244 and SS1 were fractionated into different cellular compartments (Doig and Trust 1994 Infect. Immun. 62:4526-4533: O&#39;Toole et al. 1995 J. Bacteriol. 177:6049-6057). The specific antiserum was used to probe these fractions by western immunoblot to identify in which fraction the protein was localized. In all cases, the immunoreactive protein was present in the outer membrane as had been predicted by the sequence features and motif searches described herein.  
     [0511] Demonstration of Protein Efficacy as a Vaccine  
     [0512] Purification of vac36 (SEQ ID NO: 5146) for Efficacy Studies  
     [0513] All the following steps were carried out at 4° C. Cell pellets were resuspended in 5 volumes per gram of cell of lysis buffer (50 mM Sodium Phosphate pH 8.0, 0.5 M NaCl, 5 mM Imidazole) with 10 mM EDTA, 1 mM phenylmethylsulfonyl fluoride (PMSF) and 0.1% β-mercaptoethanol, and ruptured by several passages through a small volume microfluidizer (Model M-110S, Microfluidics International Corporation, Newton, Mass.). The resulting homogenate was made 0.2% sodium deoxycholate (DOC), stirred 20 minutes, then centrifuged (10,000 g×30 nm). The pellets were washed twice with Lysis Buffer containing 10 mM EDTA, 1% Triton X-100, 1 mM PMSF and 0.1% β-mercaptoethanol, then with lysis buffer containing 1 M urea, 1 mM PMSF and 0.1% β-mercaptoethanol. The resulting white pellet is composed primarily of inclusion bodies, free of unbroken cells and membranous materials.  
     [0514] The inclusion bodies were dissolved in 20 ml 6M guanidine-HCl in lysis buffer with 1 mM PMSF and 0.1% β-mercaptoethanol, and incubated on ice for 1 hour. Materials that did not dissolve were removed by centrifugation (100,000 g×30 min.) The clear supernatant was filtered through a 0.8 μm Supor filter (Gelman Sciences, FRG) and then load directly onto a 10 ml Ni 2+ -NTA agarose column (Hochuli et al. 1987) pre-equilibrated in 6M guanidine-HCl in Lysis Buffer containing 1 mM PMSF and 0.1% β-Mercaptoethanol. The column was washed with 20 ml (2 bed volumes) of Lysis Buffer containing 6M guanidine-HCl, 1 mM PMSF and 0.1% β-mercaptoethanol, then guanidine-HCl was removed slowly with a 100 ml linear gradient (from 6M to 0 M Guanidine-HCl) of lysis buffer containing 0.5% Brij 35, 1 mM PMSF, 0.1% β-mercaptochanol. Next, the column was developed with a 25 ml linear gradient of increasing imidazole (5 to 500 mM) in Lysis buffer containing 0.5% Brij 35, 1 mM PMSF and 0.1% β-mercaptoethanol. The recombinant proteins elute as a peak centered at 100 mM imidazole.  
     [0515] Fractions containing the recombinant proteins were pooled and then concentrated to approximately 8 ml by centrifugal filtration (Centriprep-10, Amicon, Mass.), and loaded directly onto a 350-ml column (2.2×91 cm) of Sephacyl S-100 HR gel filtration medium equilibrated in Buffer A (50 mM Sodium Phosphate, pH 8.0, 500 mM NaCl, 0.1 mM EGTA, 1 mM PMSF, 0.1%β-mercaptoethanol, 0.5% Brij 35) and ran in Buffer A at 30 ml/h. Fractions containing the recombinant protein were identified by absorbance at 280 nm and analyzed by SDS-PAGE. Fractions were pooled, concentrated to 1.5 to 2 mg/ml and dialysed overnight against 10 mM Potassium Phosphate pH 7.5, 150 mM NaCl, 0.1 mM EGTA and 0.5% Brij 35. The concentration of protein in the dialysate was quantified, then aliquoted prior to freezing at −20° C.  
     [0516] Mouse Model of  Heliocobacter Pylori  Infection  
     [0517] A murine model of  H. pylori  infection was produced by infection of C57BL/6 mice with with  H. pylori  Sydney strain SS1 and was used to assess the efficacy of recombinant  H. pylori  vac36. This mouse-adapted  H. pylori  strain is cagA+ vacA+, shows colonization levels in C57BL/6 mice equivalent to those observed in humans, forms adhesion pedestals, colonizes for at least 8 months, and elicits a chronic-active gastritis and mucosal atrophy (Lee et al., Gastroenterology, 112:1386-1397, 1997). Dose-response studies have shown 100% infection rates of inbred C57BL/6 and Balb/C mice at 8 weeks post-challenge with a single inoculation of 106 organisms.  
     [0518] Animals  
     [0519] Female SPF BALB/c mice were purchased from Bomholt Breeding center (Denmark). They were kept in ordinary makrolon cages with free supply of water and food. The animals were 4-6 weeks old at arrival.  
     [0520] Infection  
     [0521] After a minimum of one week of acclimatization, the animals were infected with a type 2 strain (VacA negative) of  H. pylori  (strain 244, originally isolated from an ulcer patient). In our hands, this strain has earlier proven to be a good colonizer of the mouse stomach. The bacteria were grown overnight in Brucella broth supplemented with 10% fetal calf serum, at 37 C in a microaerophilic atmosphere (10% CO 2 , 5% O 2 ). The animals were given an oral dose of omeprazole (400 μmol/kg) and 3-5 h after this an oral inoculation of  H. pylori  in broth (approximately 10 8  cfu/animal). Positive take of the infection was checked in some animals 2-3 weeks after the inoculation.  
     [0522] Antigens  
     [0523] Recombinant  H. pylori  antigens were chosen based on their association with externally exposed  H. pylori  cell membrane. These antigens were selected from the following groups: (1.) Outer Membrane Proteins; (2.) Periplastic/Secreted proteins; (3.) Outer Surface proteins; and (4.) Inner Membrane proteins. All recombinant proteins were constructed with a hexa-HIS tag for purification reasons and the non-Helicobacter pylori control protein (β-galactosidase from  E. coli ; LacZ), was constructed in the same way.  
     [0524] All antigens were given in a soluble form, i.e. dissolved in either a HEPES buffer or in a buffer containing 0.5% Deoxycholate (DOC).  
     [0525] The antigens are listed in Table 6 below.  
               TABLE 6                         Helicobacter pylori  proteins                                    Outer membrane Proteins       SEQ ID NO: 8015       SEQ ID NO: 5379       SEQ ID NO: 9048       SEQ ID NO: 5181       SEQ ID NO: 9642       Periplastic/Secreted proteins       SEQ ID NO: 5933       Other cell envelope proteins       SEQ ID NO: 9730       SEQ ID NO: 5231       Flagella-associated proteins       SEQ ID NO: 4851       Control proteins       β-galactosidase (LacZ)                  
 
     [0526] Immunizations  
     [0527] Ten animals in each group were immunized 4 times over a 34 day period (day 1, 15, 25 and 35). Purified antigens in solution or suspension were given at a dose of 100 μg/mouse. As an adjuvant, the animals were also given 10 μg/mouse of Cholera toxin (CT) with each immunization. Omeprazole (400 μmol/kg) was given orally to the animals 3-5 h prior to immunization as a way of protecting the antigens from acid degradation. Infected control animals received HEPES buffer+CT or DOC buffer+CT. Animals were sacrificed 2-4 weeks after final immunization. A general outline of the study is shown in Table 7 below.  
               TABLE 7                          Study outline, therapeutic immunization:       Mice were all infected with  H. pylori  strain       Ah244 at day 30. Proteins are listed by their SeqID #′                                 Mouse strain       Dates for                             Substance   n = 10   Dose/mouse   dosing               1. Controls, PBS   Balb/c   0.3 ml   0, 14, 24, 34       2. Cholera toxin, 10 μg   Balb/c   0.3 ml   0, 14, 24, 34       3. Protein 14640637 (SEQ ID NO: 8015),   Balb/c   0.3 ml   0 14, 24, 34       100 μg + CT 10 μg       4. Protein 16225006 (SEQ ID NO: 9642),   Balb/c   0.3 ml   0 14, 24, 34       100 μg + CT 10 μg       5. Protein 26054702 (SEQ ID NO: 9450),   Balb/c   0.3 ml   0 14, 24, 34       100 μg + CT 10 μg       6. Protein 26380318 (SEQ ID NO: 4851),   Balb/c   0.3 ml   0 14, 24, 34       100 μg + CT 10 μg       7. Protein 29479681 (SEQ ID NO: 5379),   Balb/c   0.3 ml   0 14, 24, 34       100 μg + CT 10 μg       8. Protein 30100332 (SEQ ID NO: 5933),   Balb/c   0.3 ml   0 14, 24, 34       100 μg + CT 10 μg       9. Protein 4721061 (SEQ ID NO: 5181),   Balb/c   0.3 ml   0 14, 24, 34       100 μg + CT 10 μg       10. Protein 4821082 (SEQ ID NO: 9730),   Balb/c   0.3 ml   0 14, 24, 34       100 μg + CT 10 μg       11. Protein 978477 (SEQ ID NO: 5231),   Balb/c   0.3 ml   0 14, 24, 34       100 μg + CT 10 μg       12. Protein 7116626 (SEQ ID NO: 9048),   Balb/c   0.3 ml   0 14, 24, 34       100 μg + CT 10 μg                  
 
     [0528] Analysis of Infection  
     [0529] Mucosal infection: The mice were sacrificed by CO 2  and cervical dislocation. The abdomen was opened and the stomach removed. After cutting the stomach along the greater curvature, it was rinsed in saline. The mucosa from the antrum and corpus of an area of 25 mm 2  was scraped separately with a surgical scalpel. The mucosa scraping was suspended in Brucella broth and plated onto Blood Skirrow selective plates. The plates were incubated under microaerophilic conditions for 3-5 days and the number of colonies was counted. The identity of  H. pylori  was ascertained by urease and catalase test and by direct microscopy or Gram staining.  
     [0530] The urease test was performed essentially as follows. The reagent, Urea Agar Base Concentrate, was purchased from DIFCO Laboratories, Detroit, Mich. (Catalog # 0284-61-3). Urea agar base concentrate was diluted 1:10 with water. 1 ml of if the diluted concentrate was mixed with 100-200 μl of actively growing  H. pylori  cells. Color change to magenta indicated that cells were urease positive.  
     [0531] The catalase test was performed essentially as follows. The reagent, N,N,N′,N′-Tetramethyl-p-Phenylenediamine, was purchased from Sigma, St. Louis, Mo. (Catalog # T3134). A solution of the regent (1% w/v in water) was prepared.  H. pylori  cells were swabbed onto Whatman filter paper and overlaid with the 1% solution. Color change to dark blue indicated that the cells were catalase positive.  
     [0532] Serum antibodies: From all mice serum was prepared from blood drawn by heart puncture. Serum antibodies were identified by regular ELISA techniques, where the specific antigens of  Helicobacter pylori  were plated.  
     [0533] Mucosal antibodies: Gentle scrapings of a defined part of the corpus and of 4 cm of duodenum were performed in 50% of the mice in order to detect the presence of antibodies in the mucous. The antibody titers were determined by regular ELISA technique as for serum antibodies.  
     [0534] Statistical analysis: Wilcoxon-Mann-Whitney sign rank test was used for determination of significant effects of the antigens on  Helicobacter pylori  colonization. P&lt;0.05 was considered significant. Because the antrum is the major colonization site for Helicobacter most emphasis was put upon changes in the antral colonization.  
     [0535] Assessment of Gastric  H. Pylori  Infection  
     [0536] The presence of  H. pylori organisms in gastric tissue was determined by culture of gastric tissue and by a quantitative urease assay. In the latter method, a longitudinal segment of antrum, representing approximately ¼ of the total antral region was placed in 1 ml of urea broth. After 4 hr, the extent of color change resulting from urea hydrolysis and increased pH was quantiated by spectrophotometric measurement of A 550  (Fox et al., Immunol. 88:400-406, 1996). The assay sensitivity is ˜10 3    H. pylori  organisms. A positive ( H. pylori -infected) gastric tissue was defined as that sample showing 2 standard deviations above the mean A 550  value derived from a group of unchallenged uninfected age-matched control mice.  
     [0537] Assessment of Local Immune Response to Immunization in Gastric Tissue  
     [0538] Longitudinal sections of gastric tissues from the esophageal to the duodenal junction were embedded in OCT embedding compound, frozen in liquid nitrogen, and cryosections immunostained with monoclonal antibodies recognizing CD4 +  or CD8 + T cells or with antisera against mouse IgA for identification of IgA containing (IgACC) plasma cells (Pappo et al., Infect. Immun. 63:1246-1252, 1995). The degree of local gastric immune response was expressed quantitatively as the number of CD4 + , CD8 +  or IgACC cells per mm 2  of gastric region examined.  
     [0539] Results  
     [0540] Antibodies in sera: All antigens tested given together with CT gave rise to a measurable specific titer in serum. The highest responses were seen with SEQ ID NOs:865, 812, 658, 447, and 820 (see FIG. 1).  
     [0541] Antibodies in mucus: In the mucus scrapings, specific antibodies against all antigens tested were seen. By far the strongest response was seen with SEQ ID NOs:685, followed by 447, 865, and 658 (see FIG. 2).  
     [0542] Therapeutic immunization effects:  
     [0543] All control animals (BALB/c mice) were well colonized with  H. pylori  (strain AH244) in both antrum and corpus of the stomach. Of the antigens tested 3 proteins (SEQ ID NOs: 812, 820, and 447) gave a good and significant reduction and/or eradication of the  H. pylori  infection. The degree of colonization of the antrum was lower following immunization with SEQ ID NOs: 880, 658, and 865 compared to control. The effect of SEQ ID NOs:465, 677, and 685 did not differ from control. The control protein lacZ, i.e. the non- H. pylori  protein, had no eradication effect and in fact had higher Helicobacter colonization compared to the HEPES+CT control. All data are shown in FIGS. 3 and 4 for proteins dissolved in HEPES and DOC respectively. Data is shown as geometric mean values. n=8-10 Wilcoxon-Mann-Whitney sign rank test *=p&lt;0.05;×/10=number of mice showing eradication of  H. pylori  over the total number of mice examined.  
               TABLE 8                          Recombinant vac36 antigen protects       mice from challenge with  H. pylori                   Vaccine                                 Treatment   Urease         H. pylori             Group   Activity a     p b     burden c     p b                 vac36 (SEQ ID   0.199 ± 0.080   0.0022   55,800 ± 12,599   0.0125       NO: 5146)         H. pylori  lysate   0.057 ± 0.007   0.0002   2,360 ± 955     0.0002       buffer   1.655 ± 0.420   —   131,000 ± 18,391    —                                          
 
     [0544] The data presented indicate that all of the  H. pylori  associated proteins included in this study, when used as oral immunogens in conjunction with the oral adjuvant CT, resulted in stimulation of an immune response as measured by specific serum and mucosal antibodies. A majority of the proteins led to a reduction, and in some cases complete clearance of the colonization of  H. pylori  in this animal model. It should be noted that the reduction or clearance was due to heterologous protection rather than homologous protection (the polypeptides were based on the  H. pylori  J99 strain sequence and used in the therapeutic immunization studies against a different (AH244) challenge strain), indicating the vaccine potential against a wide variety of  H. pylori  strains.  
     [0545] The highest colonization in the antrum was seen in animals treated with the non-Helicobacter protein LacZ, indicating that the effects seen with the  Helicobacter pylori  antigens were specific.  
     [0546] Taken together these data strongly support the use of these  H. pylori  proteins in a pharmaceutical formulation for the use in humans to treat and/or prevent  H. pylori  infections.  
     [0547] Protective activity of purified recombinant  H. pylori  vac36 (SEQ ID NO: 5146) antigen The ability of purified recombinant vac36 (SEQ ID NO: 5146) antigen derived from  H. pylori  to interfere with the establishment of an  H. pylori  infection was examined in mice. Groups (n=10) of 6-8 week-old female C57BL/6 mice were immunized orally 4 times at weekly intervals as follows: 1)100 μg of recombinant vac36 (SEQ ID NO: 5146) antigen and 10 μg cholera toxin (CT) adjuvant, 2) 1 mg  H. pylori  lysate antigens and 10 μg CT, and 3) 0.2 M bicarbonate buffer and 10 ug CT adjuvant. The mice were challenged 2 weeks later on 3 consecutive days by oral administration of 10 8    H. pylori  organisms. The experiment was terminated 2 weeks post-challenge, and the  H. pylori  infection level assessed by bacterial colony counts and by quantitative urease assays.  
     [0548] Oral immunization with vac36 (SEQ ID NO: 5146) antigen interfered with the establishment of  H. pylori  infection upon challenge with live  H. pylori  organisms. Mice immunized with purified recombinant vac36 (SEQ ID NO: 5146) antigen exhibited a significantly lower level of colonization by  H. pylori , as assessed by gastric urease activity and bacterial count assays (Table 8). Oral immunization with vac36 (SEQ ID NO: 5146) antigen also resulted in the generation of a local protective gastric immune response. Greater numbers of CD4 + T cells and of IgACC were recruited in the gastric tissues of vac36-immunized mice when compared with unimmunized  H. pylori -infected mice (Table 9).  
               TABLE 9                          vac36-immunized mice generate a local gastric immune response upon challenge with         H. pylori                   Vaccine                             Treatment   CD4+   CD8+   IgACC                                                     Group   cardia a     corpus   antrum   cardia   corpus   antrum   cardia   corpus   antrum               vac36   33 ± 9 a     54 ± 8*   31 ± 8   3 ± 2   0   1 ± 1    24 ± 12   79 ± 16   67 ± 13       (SEQ       ID NO:       5146)         H.     31 ± 13   36 ± 19   24 ± 8   4 ± 2   2 ± 1   2 ± 1   31 ± 9   73 ± 13*   79 ± 15         pylori         lysate       buffer   12 ± 2   27 ± 8   18 ± 4   1 ± 1   0   0    4 ± 2   30 ± 13   46 ± 14                                  
 
     [0549] V. Sequence Variance Analysis of Genes in  Helicobacter Pylori  Strains  
     [0550] Four genes were cloned and sequenced from several strains of  H. pylori  to compare the DNA and deduced amino acid sequences. This information was used to determine the sequence variation between the  H. pylori  strain, J99, and other  H. pylori  strains isolated from human patients.  
     [0551] Preparation of Chromosomal DNA.  
     [0552] Cultures of  H. pylori  strains (as listed in Table 12) were grown in BLBB (1% Tryptone, 1% Peptamin 0.1% Glucose, 0.2% Yeast Extract 0.5% Sodium Chloride, 5% Fetal Bovine Serum) to an OD 600  of 0.2. Cells were centrifuged in a Sorvall RC-3B at 3500×g at 4° C. for 15 minutes and the pellet resuspended in 0.95 mls of 10 mM Tris-HCl, 0.1 mM EDTA (TE). Lysozyme was added to a final concentration of 1 mg/ml along with, SDS to 1% and RNAse A+T1 to 0.5 mg/ml and 5 units/ml respectively, and incubated at 37° C. for one hour. Proteinase K was then added to a final concentration of 0.4 mg/ml and the sample was incubated at 55 C for more than one hour. NaCl was added to the sample to a concentration of 0.65 M, mixed carefully, and 0.15 ml of 10% CTAB in 0.7M NaCL (final is 1% CTAB/70 mM NaCL) was added followed by incubation at 65° C. for 20 minutes. At this point, the samples were extracted with chloroform:isoamyl alcohol, extracted with phenol, and extracted again with chloroform:isoamyl alcohol. DNA was precipitated with either EtOH (1.5× volumes) or isopropanol (0.6× volumes) at −70° C. for 10 minutes, washed in 70% EtOH and resuspended in TE.  
     [0553] PCR Amplification and Cloning  
     [0554] Genomic DNA prepared from twelve strains of  Helicobacter pylori  was used as the source of template DNA for PCR amplification reactions (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994). To amplify a DNA sequence containing an  H. pylori  ORF, genomic DNA (10 nanograms) was introduced into a reaction vial containing 2 mM MgCl 2 , 1 micromolar synthetic oligonucleotide primers (forward and reverse primers, see Table 10) complementary to and flanking a defined  H. pylori  ORF, 0.2 mM of each deoxynucleotide triphosphate; dATP, dGTP, dCTP, dTTP and 0.5 units of heat stable DNA polymerase (Amplitaq, Roche Molecular Systems, Inc., Branchburg, N.J., USA) in a final volume of 20 microliters in duplicate reactions.  
                   TABLE 10                          Oligonucleotide primers used for PCR amplification of H pylon DNA se-           quences.                             Outer membrane                   Proteins (SEQ ID NO:               9450)   Forward primer 5′ to 3′   Reverse Primer 5′ to 3′               Protein 26054702   5′-   5′-           (SEQ ID NO: 9450)   TTAACCATGGTGAAA   TAGAATTCGCCTCTA       (for strains AH4,   AGCGATA-3′ (SEQ ID   AAACTTTAG-3′ (SEQ       AH15, AH61, 5294,   NO:9785)   ID NO:9786)       5640, AH18, and               AH244)               Protein 26054702   5′-   5′-       (SEQ ID NO: 9450)   TTAACCATGGTGAAA   TAGAATTCGCATAAC       (for strains AH5, 5155,   AGCGATA-3′ (SEQ ID   GATCAATC-3′ (SEQ ID       7958, AH24,and J99)   NO:9787)   NO:9788)       Protein 7116626 (SEQ   5′-   5′-       ID NO: 9048)   ATATCCATGGTGAGT   ATGAATTCAATTTTT           TTGATGA-3′ (SEQ ID   TATTTTGCCA-3′ (SEQ           NO:9789)   ID NO:9790)       Protein 29479681   5′-   5′-       (SEQ ID NO: 5379)   AATTCCATGGCTATC   ATGAATTCGCCAAAA           CAAATCCG-3′ (SEQ ID   TCGTAGTATT-3′ (SEQ           NO:9791)   ID NO:9792)       Protein 36126938   5′-   5′-       (SEQ ID NO: 5122)   GATACCATGGAATTT   TGAATTCGAAAAAGT           ATGAAAAAG-3′ (SEQ   GTAGTTATAC-3′ (SEQ           ID NO:9793)   ID NO:9794)                  
 
     [0555] The following thermal cycling conditions were used to obtain amplified DNA products for each ORF using a Perkin Elmer Cetus/GeneAmp PCR System 9600 thermal cycler:  
     [0556] Protein 7116626 (SEQ ID NO: 9048) and Protein 36126938 (SEQ ID NO: 5122);  
     [0557] Denaturation at 94° C. for 2 min,  
     [0558] 2 cycles at 94° C. for 15 sec, 30° C. for 15 sec and 72° C. for 1.5 min  
     [0559] 23 cycles at 94° C. for 15 sec, 55° C. for 15 sec and 72° C. for 1.5 min  
     [0560] Reactions were concluded at 72° C. for 6 minutes.  
     [0561] Protein 26054702 (SEQ ID NO: 9450) for strains AH5, 5155, 7958, AH24, and J99;  
     [0562] Denaturation at 94° C. for 2 min,  
     [0563] 2 cycles at 94° C. for 15 sec, 30° C. for 15 sec and 72° C. for 1.5 min  
     [0564] 25 cycles at 94° C. for 15 sec, 55° C. for 15 sec and 72° C. for 1.5 min  
     [0565] Reaction was concluded at 72° C. for 6 minutes.  
     [0566] Protein 26054702 (SEQ ID NO: 9450) and Protein 29479681 (SEQ ID NO: 5379) for strains AH4, AH15, AH61, 5294, 5640, AH18, and Hp244;  
     [0567] Denaturation at 94 C for 2 min,  
     [0568] 2 cycles at 94° C. for 15 sec, 30° C. for 20 sec and 72° C. for 2 min  
     [0569] 25 cycles at 94° C. for 15 sec, 55° C. for 20 sec and 72° C. for 2 min  
     [0570] Reactions were concluded at 72° C. for 8 minutes.  
     [0571] Upon completion of thermal cycling reactions, each pair of samples were combined and used directly for cloning into the pCR cloning vector as described below.  
     [0572] Cloning of  H. Pylori  DNA Sequences into the pCR TA Cloning Vector.  
     [0573] All amplified inserts were cloned into the pCR 2.1 vector by the method described in the Original TA cloning kit (Invitrogen, San Diego, Calif.). Products of the ligation reaction were then used to transform the TOP10F′ (INVaF′ in the case of  H. pylori  sequence 350) strain of  E. coli  as described below.  
     [0574] Transformation of Competent Bacteria with Recombinant Plasmids  
     [0575] Competent bacteria,  E coli  strain TOP10F′ or  E. coli  strain INVaF′ were transformed with recombinant pCR expression plasmids carrying the cloned  H. pylori  sequences according to standard methods (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994). Briefly, 2 microliters of 0.5 micromolar BME was added to each vial of 50 microliters of competent cells. Subsequently, 2 microliters of ligation reaction was mixed with the competent cells and incubated on ice for 30 minutes. The cells and ligation mixture were then subjected to a “heat shock” at 42° C. for 30 seconds, and were subsequently placed on ice for an additional 2 minutes, after which, samples were incubated in 0.45 milliliters SOC medium (0.5% yeast extract, 2.0% tryptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgC12, 10 mM MgSO4 and 20, mM glucose) at 37° C. with shaking for 1 hour. Samples were then spread on LB agar plates containing 25 microgram/ml kanamycin sulfate or 100 micrograms/ml ampicillan for growth overnight. Transformed colonies of TOP10F′ or INVaF′ were then picked and analyzed to evaluate cloned inserts as described below.  
     [0576] Identification of Recombinant PCR Plasmids Carrying  H. pylori  Sequences  
     [0577] Individual TOP10F′ or INVaF′ clones transformed with recombinant pCR- H. pylori  ORFs were analyzed by PCR amplification of the cloned inserts using the same forward and reverse primers, specific for each  H. pylori  sequence, that were used in the original PCR amplification cloning reactions. Successful amplification verified the integration of the  H. pylori  sequences in the cloning vector (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994).  
     [0578] Individual clones of recombinant pCR vectors carrying properly cloned  H. pylori  ORFs were picked for sequence analysis. Sequence analysis was performed on ABI Sequencers using standard protocols (Perkin Elmer) using vector-specific primers (as found in PCRII or pCR2.1, Invitrogen, San Diego, Calif.) and sequencing primers specific to the ORF as listed in Table 11 below.  
               TABLE 11                          OligonucIeotide primers used for sequencing of H vylori DNA sequences.                             Outer membrane                   Proteins   Forward primers 5′ to 3′   Reverse Primers 5′ to 3′               Protein 26054702   5′CCCTTCATTTTAGAAA   5′CTTTGGGTAAAAACGCA           (SEQ ID NO: 9450)   TCG-3′ (SEQ ID NO:9795)   TC-3′ (SEQ ID NO:9802)           5′ATTTCAACCAATTCAA   5′CGATCTTTGATCCTAAT           TGCG-3′ (SEQ ID   TCA-3′ (SEQ ID NO:9803)           NO:9796)   5′ATCAAGTTGCCTATGCT           5′GCCCCTTTTGATTTGA   GA-3′ (SEQ ID NO:9804)           AGCT-3′ (SEQ ID               NO:9797)               5′TCGCTCCAAGATACCA               AGAAGT-3′ (SEQ ID               NO:9798)               5′CTTGAATTAGGGGCA               AAGATCG-3′ (SEQ ID               NO:9799)               5′ATGCGTTTTTACCCAA               AGAAGT-3′ (SEQ ID               NO:9800)               5′ATAACGCCACTTCCTT               ATTGGT-3′ (SEQ ID               NO:9801)           Protein 7116626   5′TTGAACACTTTTGATT   5′GTCTTTAGCAAAAATGG       (SEQ ID NO: 9048)   ATGCGG-3′ (SEQ ID   CGTC-3′ (SEQ ID NO:9807)           NO:9805)   5′AATGAGCGTAAGAGAG           5′GGATTATGCGATTGTT   CCTTC-3′ (SEQ ID NO:9808)           TTACAAG-3′ (SEQ ID               NO:9806)           Protein   5′CTTATGGGGGTATTGT   5′AGGTTGTTGCCTAAAGA       29479681 (SEQ ID NO: 5379)   CA-3′ (SEQ ID NO:9809)   CT-3′ (SEQ ID NO:9811)           5′AGCATGTGGGTATCC   5′-           AGC-3′ (SEQ ID NO:9810)   CTGCCTCCACCTTTGATC-               3′ (SEQ ID NO:9812)       Protein 36126938   5′ACCAATATCAATTGGC   5′CTTGCTTGTCATATCTA       (SEQ ID NO: 5122)   ACT-3′ (SEQ ID NO:9813)   GC-3′ (SEQ ID NO:9815)           5′ACTTGGAAAAGCTCT   5′-           GCA-3′ (SEQ ID NO:9814)   GTTGAAGTGTTGGTGCTA-               3′ (SEQ ID NO:9816)           5′CAAGCAAGTGGTTTG   5′GCCCATAATCAAAAAGC           GTTTTAG-3′ (SEQ ID   CCAT-3′ (SEQ ID NO:98 19)           NO:98 17)   5′CTAAAACCAAACCACTT           5′TGGAAAGAGCAAATC   GCTTGTC-3′ (SEQ ID           ATTGAAG-3′ (SEQ ID   NO:9820)           NO:9818)           Vector Primers   5′-   5′-           GTAAAACGACGGCCAG-   CAGGAAACAGCTATGAC-           3′ (SEQ ID NO:9821)   3′ (SEQ ID NO:9822)                  
 
     [0579] Results  
     [0580] To establish the PCR error rate in these experiments, five individual clones of Protein 26054702 (SEQ ID NO: 9450), prepared from five separate PCR reaction mixtures from  H. pylori  strain J99, were sequenced over a total length of 897 nucleotides for a cumulative total of 4485 bases of DNA sequence. DNA sequence for the five clones was compared to a DNA sequence obtained previously by a different method, i.e., random shotgun cloning and sequencing. The PCR error rate for the experiments described herein was determined to be 2 base changes out of 4485 bases, which is equivalent to an estimated error rate of less than or equal to 0.04%.  
     [0581] DNA sequence analysis was performed on four different open reading frames identified as genes and amplified by PCR methods from a dozen different strains of the bacterium  Helicobacter pylori . The deduced amino acid sequences of three of the four open reading frames that were selected for this study showed statistically significant BLAST homology to defined proteins present in other bacterial species. Those ORFs included: Protein 26054702 (SEQ ID NO: 9450), homologous to the val A &amp; B genes encoding an ABC transporter in F. novicida; Protein 7116626 (SEQ ID NO: 9048), homologous to lipoprotein e (P4) present in the outer membrane of  H. influenzae ; Protein 29479681 (SEQ ID NO: 5379), homologous to fecA, an outer membrane receptor in iron (III) dicitrate transport in  E. coli . Protein 36126938 (SEQ ID NO: 5122) was identified as an unknown open reading frame, because it showed low homology with sequences in the public databases.  
     [0582] To assess the extent of conservation or variance in the ORFs across various strains of  H. pylori , changes in DNA sequence and the deduced protein sequence were compared to the DNA and deduced protein sequences found in the J99 strain of  H. pylori  (see Table 12 below). Results are presented as percent identity to the J99 strain of  H. pylori  sequenced by random shotgun cloning. To control for any variations in the J99 sequence each of the four open reading frames were cloned and sequenced again from the J99 bacterial strain and that sequence information was compared to the sequence information that had been collected from inserts cloned by random shotgun sequencing of the J99 strain. The data demonstrate that there is variation in the DNA sequence ranging from as little as 0.12% difference (Protein 36126938 (SEQ ID NO: 5122), J99 strain) to approximately 7% change (Protein 26054702 (SEQ ID NO: 9450), strain AH5). The deduced protein sequences show either no variation (Protein 36126938 (SEQ ID NO: 5122), strains AH18 and AH24) or up to as much as 7.66% amino acid changes (Protein 26054702 (SEQ ID NO: 9450), Strain AH5).  
               TABLE 12                          Multiple Strain DNA Sequence analysis of  H. pylori  Vaccine Candidates                                                 J99 Protein #:   26054702   26054702   7116626   7116626   29479681   29479681   36126938   36126938           (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID           NO: 9450)   NO: 4688)   NO: 9048)   NO: 4286)   NO: 5379)   NO: 617)   NO: 5122)   NO: 360)                 Length of Region                                                 Sequenced:   248 a.a.   746 nt.   232 a.a.   696 nt.   182 a.a.   548 nt.   273 a.a.   819 nt.       Strain Tested   AA   Nuc.   AA   Nuc.   AA   Nuc.   AA   Nuc.           identity   identity   identity   identity   identity   identity   identity   identity                                                         J99   100.00%   100.00%   100.00%   100.00%   100.00%   100.00%   99.63%   99.88%       AH244   95.16%   95.04%   n.d.   n.d.   99.09%   96.71%   98.90%   96.45%       AH4   95.97%   95.98%   97.84%   95.83%   n.d.   n.d.   97.80%   95.73%       AH5   92.34%   93.03%   98.28%   96.12%   98.91%   96.90%   98.53%   95.73%       AH15   95.16%   94.91%   97.41%   95.98%   99.82%   97.99%   99.63%   96.09%       AH61   n.d.   n.d.   97.84%   95.98%   99.27%   97.44%   n.d.   n.d.       5155   n.d.   n.d.   n.d.   n.d.   99.45%   97.08%   98.53%   95.60%       5294   94.35%   94.37%   98.28%   95.40%   99.64%   97.26%   97.07%   95.48%       7958   94.35%   94.10%   97.84%   95.40%   n.d.   n.d.   99.63%   96.46%       5640   95.16%   94.37%   97.41%   95.69%   99.09%   97.63%   98.53%   95.48%       AH18   n.d.   n.d.   98.71%   95.69%   99.64%   97.44%   100.00%   95.97%       AH24   94.75%   95.04%   97.84%   95.40%   99.27%   96.71%   100.00%   96.46%                          
 
     [0583] VI. Experimental Knock-Out Protocol for the Determination of Essential  H. Pylori  Genes as Potential Therapeutic Targets  
     [0584] Therapeutic targets are chosen from genes whose protein products appear to play key roles in essential cell pathways such as cell envelope synthesis, DNA synthesis, transcription, translation, regulation and colonization/virulence.  
     [0585] The protocol for the deletion of portions of  H. pylori  genes/ORFs and the insertional mutagenesis of a kanamycin-resistance cassette in order to identify genes which are essential to the cell is modified from previously published methods (Labigne-Roussel et al., 1988, J. Bacteriology 170, pp. 1704-1708; Cover et al., 1994, J. Biological Chemistry 269, pp. 10566-10573; Reyrat et al., 1995, Proc. Natl. Acad. Sci. 92, pp 8768-8772). The result is a gene “knock-out.” 
     [0586] Identification and Cloning of  H. Pylori  Gene Sequences  
     [0587] The sequences of the genes or ORFs (open reading frames) selected as knock-out targets were identified from the  H. pylori  genomic sequence and used to design primers to specifically amplify the genes/ORFs. All synthetic oligonucleotide primers (Table 13) were designed with the aid of the OLIGO program (National Biosciences, Inc., Plymouth, Minn. 55447, USA), and were purchased from Gibco/BRL Life Technologies (Gaithersburg, Md., USA). Specific primers (F1 and R1) were chosen which flanked most or all of the ORF, depending on its size. If the ORF was smaller than 800 to 1000 base pairs, flanking primers were chosen outside of the open reading frame.  
               TABLE 13                          Oligonucleotide Sequences for Knock-Out Gene/ORFs                         Deletion-                                     Acces-   Cloning   creating   Targeting       Gene   sion   Primers   Primers   Primers                                             Name   Number   F1   R1   F2   R2   F3   R3               rnh   P2332   TTGCCCCAT   AGAGCGTAT   TCTTGCATC   CGGGTCAAA   AATCCGTTT   AACACTT           9   CGTATTGAT   TTCACCCGA   TTAATCCAC   ACGACCACT   CGCTAATTT   ATTTCCT               AGA (SEQ   AAG   TCC   TAA   AGT   ATA               ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9823)   NO: 9824)   NO: 9825)   NO: 9826)   NO: 9827)   NO: 9828       ppi   P2982   TGGTATAAG   TTGACTAAA   ATAGAGAGC   CCTTTATTG   ATGTCCGTT   TAGGGTG       B   0   GATTTGAAT   CACATGCGA   GTTGTGTTT   GTTTTGATC   GTCTGTATG   TAGGGAT               GGA   GAA   AGC   GTG   GAA   GAT               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9829)   NO: 9830)   NO: 9831)   NO: 9832)   NO: 9833)   NO: 9834       tsf   P3482   GCGTTTGGC   GAAATGGAA   GCAAATCCC   GTGGCTAAA   GCTAAAACT   GTTAGGA           8   TTCTTCGTT   AATAGCGGT   CAGCCACTT   AATGAGGGC   TCATCGCTC   TTAGAAA               GTC   CAA   CC   TT   AAT   ATTG               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9835)   NO: 9836)   NO: 9837)   NO: 9838)   NO: 9839)   NO: 9840)       Mur   P1490   GTTGGGCAG   CAAACAAAC   CATTGATGC   CGTGGTGGT   CGGGCATTG   TGGTCTATC       D   0   AAAATAAGG   CTGACAAGA   CTAAAACTT   TTTCCCGTT   TGTTTGTTT   ATGCGAATT               TGA   AAC   CG   AG   TT   AT               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9841)   NO: 9842)   NO: 9843)   NO: 9844)   NO: 9845)   NO: 9846)       Mur   P2218   GCGTTTGGG   CGCGCTAGA   GCCCTGATC   AAGGGCGTT   GGCGTTATT   TTTCACCGG       E   8   GATTTGATG   GGCTTGTAA   CATTCCCCC   TGTTTCATC   AAGCGACAT   CAATTTTAG               TTC   AA   CT   TC   CG   CC.               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9847)   NO: 9848)   NO: 9849)   NO: 9850)   NO: 9851)   NO: 9852)       Alg   P0787   GCGTTTTGA   GTAAAAACA   GCGTGTTTT   GGAATTTTA   AAATCTCTG   AATCAAAAA       A   4   TTCTGTCTG   CCGCTAACG   CTAAGGGTT   ACGCTCTTT   TGGGCTTAG   CAAGAGCGT               TTA   CAT   CA   TT   TG   GG               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9853)   NO: 9854)   NO: 9855)   NO: 9856)   NO: 9857)   NO: 9858)       met   P1935   GCCCCAGCC   GGAGGGCGC   TCACGCTTT   CGCTAATCA   TGCCCAAAA   AACGGGTTT       L   8   CCATAATAC   AATTAAACA   CTAAATCAT   CATCCTTTC   ATCCACTAA   GACACTGAT               AAA   TCG   CA   TT   CG   GA               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9859)   NO: 9860)   NO: 9861)   NO: 9862)   NO: 9863)   NO: 9864)       fus   X1627   GAATGCGGT   GCGTTTTTA   GGGCGATGT   GGATAGCCT   AAGTTTATG   GGAGCAATC       A   8   GGTTTTAGA   AGACTGAAT   GATTGGCGA   GCCAAAACG   CGGGCGAGA   AGCCATTTT               GAG   ACA   TT   CC   TT   TC               SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9865)   NO: 9866)   NO: 9867)   NO: 9868)   NO: 9869)   NO: 9870)       Flg   U0954   CTAGCGATT   CGGCCTCCT   AGCGGGCAG   GCATTGATC   ACGGGTTAG   CAAAAGAGG       E   9   CAAGGCGAT   TCAAACACA   TTTAGGACC   GCATTTTTA   CAGGGCAGA   CGGGTTCAT               GG   TT   AC   GCC   AT   GC               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9871)   NO: 9872)   NO: 9873)   NO: 9874)   NO: 9875)   NO: 9876)       Fli   M3769   TTTAGAAGT   CATACACGC   AGTGTGGTC   CCCCTAATA   CAAAAGATT   AATGGTTTT       M-   1   CGTTGATGA   TCACTTCAT   GCCTGTGGT   GTCTGTCAA   GAAGCAGAA   CCTATACCC       set       GA   CG   GGAG   TCAT   GAGT   TTGA       A       (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9877)   NO: 9878)   NO: 9879)   NO: 9880)   NO: 9881)   NO: 9882)       Fli   M3769   GAGAGCAAA   CATACACGC   AGTGTGGTC   CCCCTAATA   CAAAAGATT   AATGGTTTT       M-   1   TCCTTATCC   TCACTTCAT   GCCTGTGGT   GTCTGTCAA   GAAGCAGAA   CCTATACCC       set       AG   CC   GGAG   TCAT   GACT   TTGA       B       (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9883)   NO: 9884)   NO: 9885)   NO: 9886)   NO: 9887)   NO: 9888)       Mur   U3279   TTGAAACCC   TCAACTGAT   GCTAGGATT   TACGAGACA   ATAGGCATG   CCATTACAT       C   4   CAAAAGTTT   AGGTAATAT   TATGCCAAT   AAATAGGGA   CAGAATTTT   TTCGCCTC               TAC   CCC   TTA   TTT   TCC   (SEQ ID               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   NO: 9894)               NO: 9889)   NO: 9890)   NO: 9891)   NO: 9892)   NO: 9893)       dna   M1004   CGATAGATA   GGGCTTGTA   GTTTTAAAA   TTCTAAAAG   TAAGTCAAG   TTTTGGGGT       E   0   TTGTAGAAG   TTCATTTTG   ACGCCATAG   GTGGTAATC   CCATAAAAC   AAAAAGGCT               TCA   TAA   CCA   TTC   CAAA   GAA               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9895)   NO: 9896)   NO: 9897)   NO: 9898)   NO: 9899)   NO: 9900)       Ser   X0501   ATCTTTTTG   AGACAGCAC   CAGCCACAC   GTAAGGCGT   GCCCCATTA   AAAGGATAC       S   7   CCCTTGCTC   CAGTTTGAT   TTCAATGTC   TAGAAAAAT   AAATCCTTT   AAGGGGGA               ATA   AAA   TAT   ACC   TCT   (SEQ ID               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   NO: 9906)               NO: 9901)   NO: 9902)   NO: 9903)   NO: 9904)   NO: 9905)       gly   P0096   CTCGCTCCA   TTTTTTAGG   TGTTTGGAA   CTTTTGGGG   TTTGATAAA   TTTCAAAAC           0   TTTTATCTT   GAGGATTGA   ATGCTGGTG   GAGTTTGAC   CGCCCACTT   GCTCACCTT               TTA   GAT   ATC   AAG   TTT   TTG               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9907)   NO: 9908)   NO: 9909)   NO: 9910)   NO: 9911)   NO: 9912)       Glt   L1458   TCTATTCTT   ATAATGAGT   ACAATAATA   AATTAGCCC   AACAACCGC   CTTCAGCGA       x   0   TTGATGCTC   TTGATCGTT   GGCTTTGTC   TTAAAATAG   TAAAATCAA   TACTAAAAG               TCT   ACG   TTC   ATG   AC   AT               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9913)   NO: 9914)   NO: 9915)   NO: 9916)   NO: 9917)   NO: 9918)       Sig   M3769   TAGGGGCGA   GCTGGATAA   TTTTTGGGG   GGCTGGTAA   AGGCTATTC   ATTCTCATC       28   1   TTGAAAACA   GGATTTGCT   GTATGCTAA   ATACTGGAT   AAGGTGGCT   AACGACTTC       (fliA)       GC   CT   PA   AG   AAA   TAAA               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9919)   NO: 9920)   NO: 9921)   NO: 9922)   NO: 9923)   NO: 9924)       Sig   M7344   GCAGTTGGC   GAGAGCGAA   TGATTGTTG   AAAATCGGT   CTTTTCCTT   AAAACAAAC       54   3   GGTATTTGG   GTTTATGAG   GGTAGCTCT   CTGATGCTC   TCGCTTGAA   GCATCAAAA               TG   PA   CA   TTA   GA   AT               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9925)   NO: 9926)   NO: 9927)   NO: 9928)   NO: 9929)   NO: 9930)       Mur   U1240   CGCCCGAAT   TGATGCAAC   GGGATTTTA   CAAGCGGCA   TTTTTAAGG   TGGGTTTTA       I   5   GGATGAGTA   AAATGGACG   GAATACTTT   AGTGGAAGA   GCGTATTTT   AGGAATGTG               GG (SEQ   ACA   TGC (SEQ   TA (SEQ   TGT (SEQ   ATG (SEQ               ID   (SEQ ID   ID   ID   ID   ID               NO: 9931)   NO: 9932)   NO: 9933)   NO: 9934)   NO: 9935)   NO: 9936)       dna   D2618   CGCGCTCAA   GGCCCATTC   GCCCCATTC   CGCTTTAAC   AGCGTTTTT   TCCCTATCA       B   5   AATCCCTAA   TTTCGGATA   CTGTTTTTA   GCTCCTTTC   GTAAGGGGG   TAGCGTTAG               AT   TT   GC   AC   TAT   TGC               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9937)   NO: 9938)   NO: 9939)   NO: 9940)   NO: 9941)   NO: 9942)       Mur   D1060   CTTAGGGGT   GCACAATTC   CCAAAGCTA   GCTCATGGA   CTTAGCCCC   CGCAAAAGG       G   2   TTTTAGCAT   CCACACGCT   AAGCGGTGT   TATAAAGGG   TTTAGTGTT   GTAGGGGAT               GAA   GC   TTT   GTATT   TA   PA               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9943)   NO: 9944)   NO: 9945)   NO: 9946)   NO: 9947)   NO: 9948)       Ipx   U3279   TTTTATTTT   CAAACTTAT   AAAGATAAC   TAATTCTAC   GCGGTCATG   ATTCAAAGA       C   4   TAGAAACGA   CGCCCTCTC   GCTAGGATT   AGAGTGGTT   GAATTTTTA   AAGCTGGCT               ATC   TA   TCTAC   AATGG   GA   GTCT               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9949)   NO: 9950)   NO: 9951)   NO: 9952)   NO: 9953)   NO: 9954)       kdt   M8630   GCTTGTGGG   GAACCCCCT   ACCATGCTC   GTAAGTTTG   AAAAAGAAA   AAAGATACT       A   5   GGTTGTTTT   AAAATGACA   ATTAACGCT   AGCGGCTAA   GAAGAACTC   CCCCTGTGA               AT   AT   AGG   TTC   GTG   TTA               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9955)   NO: 9956)   NO: 9957)   NO: 9958)   NO: 9959)   NO: 9960)       Ipx   U0954   CAAAGAAAC   GCATGGTAT   GCAGCGGCA   CGCCCCCAA   AAAGGTTTG   CACTTGAGC       B   9   GCAAAATAC   TCAGCGTTT   CAGCGACTT   AAAGTCGCA   AAACAAGAA   GTTAGCAAC               AG   TC   TAG   GTA   ATCT   AAT               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9961)   NO: 9962)   NO: 9963)   NO: 9964)   NO: 9965)   NO: 9966)       KO       GGGGTGTTT   CGCTAGGAG   CAAGGGCGT   GGGATTGTT   GGAATACAA   GCCTTTTTA       24       GAAATTGAT   AAAGGAAGG   TTTTTGGGG   ACAGGAAAA   TAACGCATA   GACAACCCT               AGA   AAA   TAT   GAT   AAT   ACT               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9967)   NO: 9968)   NO: 9969)   NO: 9970)   NO: 9971)   NO: 9972)       KO       CCCCTAAAC   GCTAGAAAT   GCGATTATG   TTATTGTGG   TATGCGGCT   CCCTAAATC       26       TCAAATCTC   GCCATGAGA   GGGTATTTA   AGTTGCTTG   CATCCTATT   CAAATCAAG               AAT   AAG   TTG   TCA   AAA   CAG               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID               NO: 9973)   NO: 9974)   NO: 9975)   NO: 9976)   NO: 9977)   NO: 9978)       K02       CCCCCTAAC   CAAACGATA   TCCAGCTTG   GGTGGTGAT   TTTGGTTGC   TTTTGACAG       7       CCCATAATA   GAAAGAAAT   AAATCCAAA   TAAAAACCT   TTGGTTTGA   ACGATGACA               CCG   ACG (SEQ   TCC (SEQ   TATTG   TAG (SEQ   GAA (SEQ               (SEQ ID   ID NO:   ID NO:   (SEQ ID   ID NO:   ID NO:               NO: 9979)   9980)   9981)   NO: 9982)   9983)   9984)       fts       TTTGAGGGT   TGGTTTTAC   ATTTTGATT   CGCTTTTTT   AAGATTACG   CGCTTTTTT       Y       GCTTATTTT   CCGCCCCAT   GGTTTTGAT   TCTTTAACC   CCCCCACCT   ATGCTGTTT               ACT   TA   ATTCA   TCTTC   TTA (SEQ   TAA (SEQ               (SEQ ID   (SEQ ID   (SEQ ID   (SEQ ID   ID NO:   ID NO:               NO: 9985)   NO: 9986)   NO: 9987)   NO: 9988)   9989)   9990)       26K       AATACGCTT   TCCACCATG   CACTTGTTC   GACTTGCCA   CCCACTTTA   CAAGCAGTA       Da_       GAATATGTT   TTTCCGCTG   GCTGTCAAT   TTAGGTAGG   AGCTCACGA   AAAATGAAA       pro       CAT   ATT (SEQ   AG (SEQ   AA (SEQ   GTGA (SEQ   CTAT (SEQ       t       (SEQ ID   ID NO:   ID NO:   ID NO:   ID NO:   ID NO:               NO: 9991)   9992)   9993)   9994)   9995)   9996)       K02       GCTTTTTAG   ATAAGGTTT   CTCTTGTTG   CATTCGGGT   TAGGACTTC   TTTATCCAA       8       CGTCATAGA   TGTTTGCGG   TATTCAATG   TATTTTGAC   ATAACTTTC   ATCATCTCT               TAT (SEQ   TAG (SEQ   AAACT   TAT (SEQ   ATCAT   CAAG (SEQ               ID NO:   ID NO:   (SEQ ID   ID NO:   (SEQ ID   ID NO:               9997)   9998)   NO: 9999)   10000)   NO:   10002)                               10001)       VAC       TTCTATTCG   ATCAATTTT   CGGCCCAAA   CGGTAAAAG   TTCTGGTGG   CCCATCCCA       51       CCTAAAACC   CAGCCCTAA   ATTAGACAG   CGTTCGCAT   TTTAGCTCG   CCCCTAGAG               TAC (SEQ   CTA (SEQ   CC (SEQ   CC (SEQ   TTTGG   ATCC (SEQ               ID NO:   ID NO:   ID NO:   ID NO:   (SEQ ID   ID NO:               10003)   10004)   10005)   10006)   NO:   10008)                               10007)       mur       CTCGTTACA   GAATGCCAT   CGGGTTTAA   CTAACGCCC   CGAACATGC   GGCTCTGTT       B       GCAGCGTGA   ATTCTTGTA   TGGCGTTGT   CTAAAGTGC   TTTTAGATT   CTCATCAAT               AA (SEQ   GC (SEQ   TT (SEQ   CA (SEQ   AC (SEQ   AG (SEQ               ID NO:   ID NO:   ID NO:   ID NO:   ID NO:   ID NO:               10009)   10010)   10011)   10012)   10013)   10014)                  
 
     [0588] Genomic DNA prepared from the  Helicobacter pylori  HpJ99 strain (ATCC 55679; deposited by Genome Therapeutics Corporation, 100 Beaver Street, Waltham, Mass. 02154) is used as the source of template DNA for amplification of the ORFs by PCR (polymerase chain reaction) (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994). For the preparation of genomic DNA from  H. pylori , see Example I. PCR amplification is carried out by introducing 10 nanograms of genomic HpJ99 DNA into a reaction vial containing 10 mM Tris pH 8.3, 50 mM KCl, 2 mM MgCl 2 , 2 microMolar synthetic oligonucleotide primers (forward=F1 and reverse=R1), 0.2 mM of each deoxynucleotide triphosphate (dATP, dGTP, dCTP, dTTP), and 1.25 units of heat stable DNA polymerase (Amplitaq, Roche Molecular Systems, Inc., Branchburg, N.J., USA) in a final volume of 40 microliters. The PCR is carried out with Perkin Elmer Cetus/GeneAmp PCR System 9600 thermal cyclers.  
               TABLE 14                       PCR Conditions                                    MurC (SEQ ID NO: 7608)       Denaturation at 94° C. for 2 min.,       25 cycles of 94° C. for 15 sec, 48° C. for 15 sec, 72° C. for 1 min, 30 sec,       Final Extension of 72° C. for 20 minutes.       Sig54 (SEQ ID NO: 7023)       Denaturation at 94° C. for 2 min.,       25 cycles of 94° C. for 15 sec, 50° C. for 15 sec, 72° C. for 1 min, 30 sec,       Final Extension of 72° C. for 20 minutes.       lpxC (SEQ ID NO: 7767)       Denaturation at 94° C. for 2 min.,       32 cycles of 94° C. for 15 sec, 50° C. for 15 sec, 72° C. for 1 min, 30 sec,       Final Extension of 72° C. for 20 minutes.       KO24,(SEQ ID NO: 8390; KO27 SEQ ID NO: 8850)       Denaturation at 94° C. for 2 min.,       25 cycles of 94° C. for 15 sec, 50.5° C. for 20 sec, 72° C. for 2 min,       Final Extension of 72° C. for 20 minutes.       KO29, (SEQ ID NO: 5811; 26 kDa Protein; SEQ ID NO: 5185)       Denaturation at 94° C. for 2 min.,       28 cycles of 94° C. for 15 sec, 50.5° C. for 20 sec, 72° C. for 2 min,       Final Extension of 72° C. for 20 minutes.       DnaE (SEQ ID NO: 7200),Glycyl (SEQ ID NO: 7200)       Denaturation at 94° C. for 2 min.,       20 cycles of 94° C. for 15 sec, 51° C. for 15 sec, 72° C. for 1 min, 30 sec,       Final Extension of 72° C. for 20 minutes.       Gltx (SEQ ID NO: 7021)       Denaturation at 94° C. for 2 min.,       25 cycles of 94° C. for 15 sec, 51° C. for 15 sec, 72° C. for 1 min, 30 sec,       Final Extension of 72° C. for 20 minutes.       KO28 (SEQ ID NO: 9375)       Denaturation at 94° C. for 2 min.,       25 cycles of 94° C. for 15 sec, 51° C. for 15 sec, 72° C. for 2 min,       Final Extension of 72° C. for 20 minutes.       KO30 (SEQ ID NO: 5216)       Denaturation at 94° C. for 2 min.,       25 cycles of 94° C. for 15 sec, 51.5° C. for 15 sec, 72° C. for 1 min, 45 sec,       Final Extension of 72° C. for 20 minutes.       MurI (SEQ ID NO: 7635), MurG (SEQ ID NO: 7605)       Denaturation at 94° C. for 2 min.,       25 cycles of 94° C. for 15 sec, 52° C. for 15 sec, 72° C. for 1 min, 30 sec,       Final Extension of 72° C. for 20 minutes.       DnaB (SEQ ID NO: 7419), KdtA (SEQ ID NO: 85610),LpxB (SEQ ID NO: 6957)       Denaturation at 94° C. for 2 min.,       27 cycles of 94° C. for 15 sec, 52° C. for 15 sec. 72° C. for 1 min, 30 sec,       Final Extension of 72° C. for 20 minutes.       Tsf (SEQ ID NO: 7109),FlgE (SEQ ID NO: 4820), FliM (SEQ ID NO: 4795),Sig28       (SEQ ID NO: 7310),MurB (SEQ ID NO: 8208)       Denaturation at 94° C. for 2 min.,       30 cycles of 94° C. for 15 sec, 52° C. for 15 sec, 72° C. for 1 min, 30 sec,       Final Extension of 72° C. for 20 minutes.       PpiB (SEQ ID NO: 7674)       Denaturation at 94° C. for 2 min.,       30 cycles of 94° C. for 15 sec, 52° C. for 15 sec, 72° C. for 2 min, 30 sec,       Final Extension of 72° C. for 20 minutes.       MurD (SEQ ID NO: 7597),MurE (SEQ ID NO: 7639), AlgA (SEQ ID NO: 6351),MetL       (SEQ ID NO: 9467),FusA (SEQ ID NO: 7042),SerS (SEQ ID NO: 7182),RnhA (SEQ       ID NO: 6992)       Denaturation at 94° C. for 2 min.,       30 cycles of 94° C. for 15 sec, 55° C. for 15 sec, 72° C. for 1 min, 30 sec,       Final Extension of 72° C. for 20 minutes.                  
 
     [0589] Upon completion of thermal cycling reactions, each sample of amplified DNA is visualized on a 2% TAE agarose gel stained with Ethidium Bromide (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994) to determine that a single product of the expected size had resulted from the reaction. Amplified DNA is then washed and purified using the Qiaquick Spin PCR purification kit (Qiagen, Gaithersburg, Md., USA).  
     [0590] PCR products are cloned into the pT7Blue T-Vector (catalog#69820-1, Novagen, Inc., Madison, Wis., USA) using the TA cloning strategy (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994). The ligation of the PCR product into the vector is accomplished by mixing a 6 fold molar excess of the PCR product, 10 ng of pT7Blue-T vector (Novagen), 1 microliter of T4 DNA Ligase Buffer (New England Biolabs, Beverly, Mass., USA), and 200 units of T4 DNA Ligase (New England Biolabs) into a final reaction volume of 10 microliters. Ligation is allowed to proceed for 16 hours at 16° C.  
     [0591] Ligation products are electroporated (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994) into electroporation-competent XL-1 Blue or DH5-a  E. coli  cells (Clontech Lab., Inc. Palo Alto, Calif., USA). Briefly, 1 microliter of ligation reaction is mixed with 40 microliters of electrocompetent cells and subjected to a high voltage pulse (25 microFarads, 2.5 kV, 200 ohms) after which the samples are incubated in 0.45 ml SOC medium (0.5% yeast extract, 2% tryptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4  and 20 mM glucose) at 37° C. with shaking for 1 hour. Samples are then spread onto LB (10 g/l bacto tryptone, 5 g/l bacto yeast extract, 10 g/l sodium chloride) plates containing 100 microgram/ml of Ampicillin, 0.3% X-gal, and 100 microgram/ml IPTG. These plates are incubated overnight at 37° C. Ampicillin-resistant colonies with white color are selected, grown in 5 ml of liquid LB containing 100 microgram/ml of Ampicillin, and plasmid DNA is isolated using the Qiagen miniprep protocol (Qiagen, Gaithersburg, Md., USA).  
     [0592] To verify that the correct  H. pylori  DNA inserts had been cloned, these pT7Blue plasmid DNAs are used as templates for PCR amplification of the cloned inserts, using the same forward and reverse primers used for the initial amplification of the J99  H. pylori  sequence. Recognition of the primers and a PCR product of the correct size as visualized on a 2% TAE, ethidium bromide stained agarose gel are confirmation that the correct inserts had been cloned. Two to six such verified clones are obtained for each knock-out target, and frozen at −70° C. for storage. To minimize errors due to PCR, plasmid DNA from these verified clones are pooled, and used in subsequent cloning steps.  
     [0593] The sequences of the genes/ORFs are again used to design a second pair of primers which flank the region of  H. pylori  DNA to be either interrupted or deleted (up to 250 basepairs) within the ORFs but are oriented away from each other. The pool of circular plasmid DNAs of the previously isolated clones are used as templates for this round of PCR. Since the orientation of amplification of this pair of deletion primers is away from each other, the portion of the ORF between the primers is not included in the resultant PCR product. The PCR product is a linear piece of DNA with  H. pylori  DNA at each end and the pT7Blue vector backbone between them which, in essence, resultes in the deletion of a portion of the ORFs. The PCR product is visualized on a 1% TAE, ethidium bromide stained agarose gel to confirm that only a single product of the correct size has been amplified.  
     [0594] A Kanamycin-resistance cassette (Labigne-Roussel et al., 1988 J. Bacteriology 170, 1704-1708) is ligated to this PCR product by the TA cloning method used previously (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., editors, 1994). The Kanamycin cassette containing a Campylobacter kanamycin resistance gene is obtained by carrying out an EcoRI digestion of the recombinant plasmid pCTB8:kan (Cover et al., 1994, J. Biological Chemistry 269, pp. 10566-10573). The proper fragment (1.4 kb) is isolated on a 1% TAE gel, and isolated using the QIAquick gel extraction kit (Qiagen, Gaithersburg, Md., USA). The fragment is end repaired using the Klenow fill-in protocol, which involved mixing 4 ug of the DNA fragment, 1 microliter of dATP, dGTP, dCTP, dTTP at 0.5 mM, 2 microliter of Klenow Buffer (New England Biolabs) and 5 units of Klenow DNA Polymerase I Large (Klenow) Fragment (New England Biolabs) into a 20 microliter reaction, incubating at 30° C. for 15 min, and inactivating the enzyme by heating to 75° C. for 10 minutes. This blunt-ended Kanamycin cassette is then purified through a Qiaquick column (Qiagen, Gaithersburg, Md., USA) to eliminate nucleotides. The “T” overhang is then generated by mixing 5 micrograms of the blunt-ended kanamycin cassette, 10 mM Tris pH 8.3, 50 mM KCl, 2 mM MgCl 2 , 5 units of DNA Polymerase (Amplitaq, Roche Molecular Systems, Inc., Branchburg, N.J., USA), 20 microliters of 5 mM dTTP, in a 100 microliter reaction and incubating the reaction for 2 hours at 37° C. The “Kan-T” cassette is purified using a QIAquick column (Qiagen, Gaithersburg, Md., USA). The PCR product of the deletion primers (F2 and R2) is ligated to the Kan-T cassette by mixing 10 to 25 ng of deletion primer PCR product, 50-75 ng Kan-T cassette DNA, 1 microliter 10× T4 DNA Ligase reaction mixture, 0.5 microliter T4 DNA Ligase (New England Biolabs, Beverly, Mass., USA) in a 10 microliter reaction and incubating for 16 hours at 16° C.  
     [0595] The ligation products are transformed into XL-1 Blue or DH5-a  E. coli  cells by electroporation as described previously. After recovery in SOC, cells are plated onto LB plates containing 100 microgram/ml Ampicillin and grown overnight at 37° C. These plates are then replica plated onto plates containing 25 microgram/ml Kanamycin and allowed to grow overnight. Resultant colonies have both the Ampicillin resistance gene present in the pT7Blue vector, and the newly introduced Kanamycin resistance gene. Colonies are picked into LB containing 25 microgram/ml Kanamycin and plasmid DNA is isolated from the cultured cells using the Qiagen miniprep protocol (Qiagen, Gaithersburg, Md., USA).  
     [0596] Several tests by PCR amplification are conducted on these plasmids to verify that the Kanamycin is inserted in the  H. pylori  gene/ORF, and to determine the orientation of the insertion of the Kanamycin-resistance gene relative to the  H. pylori  gene/ORF. To verify that the Kanamycin cassette is inserted into the  H. pylori  sequence, the plasmid DNAs are used as templates for PCR amplification with the set of primers originally used to clone the  H. pylori  gene/ORFs. The correct PCR product is the size of the deleted gene/ORF but increased in size by the addition of a 1.4 kilobase Kanamycin cassette. To avoid potential polar effects of the kanamycin resistance cassette on  H. pylori  gene expression, the orientation of the Kanamycin resistance gene with respect to the knock-out gene/ORF is determined and both orientations are eventually used in  H. pylori  transformations (see below). To determine the orientation of insertion of the kanamycin resistance gene, primers are designed from the ends of the kanamycin resistance gene (“Kan-1” 5′-ATCTTACCTATCACCTCAAAT-3′ (SEQ ID NO:10015)), and “Kan-2” 5′-AGACAGCAACATCTTTGTGAA-3′ (SEQ ID NO:10016)). By using each of the cloning primers in conjunction with each of the Kan primers (4 combinations of primers), the orientation of the Kanamycin cassette relative to the  H. pylori  sequence is determined. Positive clones are classified as either in the “A” orientation (the same direction of transcription is present for both the  H. pylori  gene and the Kanamycin resistance gene), or in the “B” orientation (the direction of transcription for the  H. pylori  gene is opposite to that of the Kanamycin resistance gene). Clones which share the same orientation (A or B) are pooled for subsequent experiments and independently transformed into  H. pylori.    
     [0597] Transformation of Plasmid DNA into  H. Pylori  Cells  
     [0598] Two strains of  H. pylori  are used for transformation: ATCC 55679, the clinical isolate which provided the DNA from which the  H. pylori  sequence database is obtained, and AH244, an isolate which had been passaged in, and has the ability to colonize the mouse stomach. Cells for transformation are grown at 37° C., 10% CO 2 , 100% humidity, either on Sheep-Blood agar plates or in Brucella Broth liquid. Cells are grown to exponential phase, and examined microscopically to determine that the cells are “healthy” (actively moving cells) and not contaminated. If grown on plates, cells are harvested by scraping cells from the plate with a sterile loop, suspended in 1 ml of Brucella Broth, spun down (1 minute, top speed in eppendorf microfuge) and resuspended in 200 microliters Brucella Broth. If grown in Brucella Broth liquid, cells are centrifuged (15 minutes at 3000 rpm in a Beckman TJ6 centrifuge and the cell pellet resuspended in 200 microliters of Brucella broth. An aliquot of cells is taken to determine the optical density at 600 nm, in order to calculate the concentration of cells. An aliquot (1 to 5 OD 600  units/25 microliter) of the resuspended cells is placed onto a prewarmed Sheep-Blood agar plate, and the plate is further incubated at 37° C., 6% CO 2 , 100% humidity for 4 hours. After this incubation, 10 microliters of plasmid DNA (100 micrograms per microliter) is spotted onto these cells. A positive control (plasmid DNA with the ribonuclease H gene disrupted by kanamycin resistance gene) and a negative control (no plasmid DNA) are done in parallel. The plates are returned to 37° C., 6% CO 2  for an additional 4 hours of incubation. Cells are then spread onto that plate using a swab wetted in Brucella broth, and grown for 20 hours at 37° C., 6% CO 2 . Cells are then transferred to a Sheep-Blood agar plate containing 25 micrograms/ml Kanamycin, and allowed to grow for 3 to 5 days at 37° C., 6% CO 2 , 100% humidity. If colonies appear, they are picked and regrown as patches on a fresh Sheep-Blood agar plate containing 25 micrograms/ml Kanamycin.  
     [0599] Three sets of PCR tests are done to verify that the colonies of transform ants have arisen from homologous recombination at the proper chromosomal location. The template for PCR (DNA from the colony) is obtained by a rapid boiling DNA preparation method as follows. An aliquot of the colony (stab of the colony with a toothpick) is introduced into 100 microliters of 1% Triton X-100, 20 mM Tris, pH 8.5, and boiled for 6 minutes. An equal volume of phenol: chloroform (1:1) is added and vortexed. The mixture is microfuged for 5 minutes and the supernatant is used as DNA template for PCR with combinations of the following primers to verify homologous recombination at the proper chromosomal location.  
     [0600] TEST 1. PCR with cloning primers originally used to amplify the gene/ORF. A positive result of homologous recombination at the correct chromosomal location should show a single PCR product whose size is expected to be the size of the deleted gene/ORF but increased in size by the addition of a 1.4 kilobase Kanamycin cassette. A PCR product of just the size of the gene/ORF is proof that the gene had not been knocked out and that the transformant is not the result of homologous recombination at the correct chromosome location.  
     [0601] TEST 2. PCR with F3 (primer designed from sequences upstream of the gene/ORF and not present on the plasmid), and either primer Kan-1 or Kan-2 (primers designed from the ends of the kanamycin resistance gene), depending on whether the plasmid DNA used was of “A” or “B” orientation. Homologous recombination at the correct chromosomal location will result in a single PCR product of the expected size (i.e., from the location of F3 to the insertion site of kanamycin resistance gene). No PCR product or PCR product(s) of incorrect size(s) will prove that the plasmid had not integrated at the correct site and that the gene had not been knocked out.  
     [0602] TEST 3. PCR with R3 (primer designed from sequences downstream of the gene/ORF and not present on the plasmid) and either primer Kan-1 or Kan-2, depending on whether the plasmid DNA used was of “A” or “B” orientation. Homologous recombination at the correct chromosomal location will result in a single PCR product of the expected size (i.e., from the insertion site of kanamycin resistance gene to the downstream location of R3). Again, no PCR product or PCR product(s) of incorrect size(s) will prove that the plasmid had not integrated at the correct site and that the gene had not been knocked out.  
     [0603] Transformants showing positive results for all three tests above indicate that the gene is not essential for survival in vitro.  
     [0604] A negative result in any of the three above tests for each transformant indicates that the gene had not been disrupted, and that the gene is essential for survival in vitro.  
     [0605] In the event that no colonies result from two independent transformations while the positive control with the disrupted ribonuclease H plasmid DNA produces transformants, the plasmid DNA is further analyzed by PCR on DNA from transformant populations prior to plating for colony formation. This will verify that the plasmid can enter the cells and undergo homologous recombination at the correct site. Briefly, plasmid DNA is incubated according to the transformation protocol described above. DNA is extracted from the  H. pylori  cells immediately after incubation with the plasmid DNAs and the DNA is used as template for the above TEST 2 and TEST 3. Positive results in TEST 2 and TEST 3 would verify that the plasmid DNA could enter the cells and undergo homologous recombination at the correct chromosomal location. If TEST 2 and TEST 3 are positive, then failure to obtain viable transformants indicates that the gene is essential, and cells suffering a disruption in that gene are incapable of colony formation.  
     [0606] Genes used in these experiments have been found to be essential, non-essential, or are still in progress, as indicated in Table 15.  
               TABLE 15                          Summary       of knock-       out                     genes/ORFs   Accession                             Gene   Number   Pathway   Status               rnh   P23329   Transcription   Not essential       ppiB   P29820   Translation   Not essential       tsf   P34828   Translation   Essential       MurD   P14900   Cell envelope   Essential       MurE   P22188   Cell envelope   Essential       AlgA   P07874   Virulence/Colo-   Not essential               nization       metL   P19358   Amino acid   Not essential               biosynthesis -               aspartate               family       fusA   X16278   Translation   Essential       FlgE   U09549   Virulence/Colo-   Not essential-               nization   motility                   impaired       FliM   M37691   Virulence/Colo-   Not essential-               nization   motility                   impaired       MurC   U32794   Cell envelope   Essential       dnaE   M10040   DNA   Essential               replication       serS   X05017   Translation   Essential       gly   P00960   Translation   Essential       gltX   L14580   Translation   Essential       sig28   M37691   Regulatory   Not essential-       (fliA)       functions   motility                   impaired       sig54       Regulatory   Not essential-               functions   motility                   impaired       MurI   U12405   Cell envelope   Essential       dnaB   D26185   DNA   Essential               replication       MurG   D10602   Cell envelope   Essential       lpxC/envA   U32794   Cell envelope   Essential       kdtA   M86305   Cell envelope   Essential       lpxB   U09549   Cell envelope   Essential       KO 24       thiolase-like   Not Essential       KO 26       histone-like   Not Essential       KO 27       respiratory   Not Essential               chain NADH               dehydrogenase-               like       ftsY   P44870   Cell division   Essential       26 KDa   P21762   Antioxidant   Essential       protein       KO28       Aldose-1-   Not Essential               epimerase-like       VAC51       Gram-neg   Not Essential               porin-like       murB   P18579   Cell wall   Essential               biosynthesis                  
 
     [0607] VII. High-throughput Drug Screen Assay  
     [0608] Cloning, Expression and Protein Purification  
     [0609] Cloning, transformation, expression and purification of the  H. pylori  target gene and its protein product, e.g., an  H. pylori  enzyme, to be used in a high-throughput drug screen assay, is carried out essentially as described in Examples II and III above. Development and application of a screening assay for a particular  H. pylori  gene product, peptidyl-propyl cis-trans isomerase, is described below as a specific example.  
     [0610] Enzymatic Assay  
     [0611] The assay is essentially as described by Fisher (Fischer, G., et. al. (1984)  Biomed. Biochim. Acta  43:1101-1111). The assay measures the cis-trans isomerization of the Ala-Pro bond in the test peptide N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide (Sigma # S-7388, lot # 84H5805). The assay is coupled with α-chymotrypsin, where the ability of the protease to cleave the test peptide occurs only when the Ala-Pro bond is in trans. The conversion of the test peptide to the trans isomer in the assay is followed at 390 nm on a Beckman Model DU-650 spectophotometer. The data are collected every second with an average scanning of time of 0.5 second. Assays are carried out in 35 mM Hepes, pH 8.0, in a final volume of 400 ul, with 10 μM α-chymotrypsin (type 1-5 from bovine Pancreas, Sigma # C-7762, lot 23H7020) and 10 nM PPIase. To initiate the reaction, 10 μl of the substrate (2 mM N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide in DMSO) is added to 390 μl of reaction mixture at room temperature.  
     [0612] Enzymatic Assay in Crude Bacterial Extract.  
     [0613] A 50 ml culture of  Helicobacter pylori  (strain J99) in Brucella broth is harvested at mid-log phase (OD 600 nm ˜1) and resuspended in lysis buffer with the following protease inhibitors: 1 mM PMSF, and 10 μg/ml of each of aprotinin, leupeptin, pepstatine, TLCK, TPCK, and soybean trypsin inhibitor. The suspension is subjected to 3 cycles of freeze-thaw (15 minutes at −70° C., then 30 minutes at room temperature), followed by sonication (three 20 second bursts). The lysate is centrifuged (12,000 g×30 minutes) and the supernatant is assayed for enzymatic activity as described above.  
     [0614] Results  
     [0615] PPI from  H. pylori  was expressed in  E. coli  using the pET-28b expression vector from Novagen (cat # 69868-1). The expressed recombinant protein was isolated from the soluble fraction of bacterial cells that had been disrupted by cavitation in a Microfluidics Cell disruption chamber. The expression levels of recombinant PPI produced 100 mg of protein. The recombinant protein could be purified to homogeneity by Ni 2+  chelate chromatography and gel filtration. On sodium dodecyl sulfate polyacrylamide gels, the recombinant protein migrates as a single band at 21 kDa, in accordance with the predicted molecular weight of 20,975 deduced from the gene sequence.  
     [0616] The PPIase activity was assayed using the chromogenic tetrapeptide substrate succinyl-Ala-Ala-Pro-Phe-p-nitroanilide. An initial velocity of 4.9 μmole/min/mg protein was measured with the purified enzyme (FIG. 5). This corresponds to a k cat  of 1.6 sec −1  which is similar to the one obtained for the  E. coli  PPIase (Liu, J. and Walsh, C. T. (1990)  Proc. Natl. Acad. Sci. USA  87:4028-4032) and the one from porcine kidney (Fischer, G. (1989)  Nature  337:476-478).  
     [0617] The recombinant protein has a high catalytic efficiency of 2.06×10 9  M −1  s −1  when the assay is measured at 25° C. These values are one to two orders of magnitude higher than that observed for other characterized PPIases. However, in those studies, the ppiase assay was conducted at 10° C., which may account for the discrepency. The calalytic efficiency is very close to the 1×10 8  to 1×10 9  M −1 s   −1  upper diffusinal limit for “kinetically perfect” enzymes (Albery, W. J. and Knowles, J. R. (1976)  Biochemistry  15:5631-5640) and suggests that by at least one measure, the  H. pylori  PPIase is a highly effective catalyst in the cis-trans isomerisation of the Ala-Pro bond in the oligopeptide substrate.  
     [0618] The presence of PPIase was also determined in an  H. pylori  extract. As with the assay for the recombinant protein, PPIase activity was detected, and was dependent on the concentration of extract added (FIG. 6).  
     [0619] These results show that PPIase activity can be measured on either  H. pylori  extracts or on the recombinant protein in  E. coli . The high catalytic efficiency also demonstrates that  H. pylori  enzymes, such as PPIase, can be expressed at high levels and in an active form in  E. coli . Such high yields of purified proteins provide for the design of various high throughput drug screening assays.  
     [0620] VIII. Cloning, Purification and Characterization of the Gene Encoding the Glutamate Racemase of  H. Pylori.    
     [0621] The  Helicobacter pylori  genome contains an open reading frame (ORF) of 255 amino acids (SEQ ID NO: 7635) that was found to have homology to the  Staphylococcus haemolyticus  glutamate racemase gene (dga) (NCBI Accession number U12405) and to the  E. coli  murI gene which encodes glutamate racemase activity in that organism. To evaluate whether this  H. pylori  ORF encodes a protein with glutamate racemase activity, the gene was isolated by polymerase chain reaction (PCR) amplification cloning, overexpressed in  E coli , and the protein purified to apparent homogeneity. A simple assay for glutamate racemase activity resulting in the isomerization of D-glutamic acid to L-glutamic acid was developed to facilitate purification and for future use as a high-throughput drug screen.  
     [0622] The ORF in  H. pylori  has been found by gene disruption studies to be essential for viability of  H. pylori  cells in laboratory culture (see Example VI above). Therefore, inhibition of the enzymatic activity would be expected to be lethal for the organism, and such inhibitors may have utility in antimicrobial therapy of human infectious diseases.  
     [0623] Cloning of  H. pylori  murI Gene Encoding Glutamate Racemase  
     [0624] A 765 base pair DNA sequence encoding the murI gene of  H. pylori  was isolated by polymerase chain reaction (PCR) amplification cloning. A synthetic oligonucleotide primer (5′-AAATAGTCATATGAAAATAGGCGTTTTTG-3′ (SEQ ID NO:10017)) encoding an NdeI restriction site and the 5′ terminus of the murI gene and a primer (5′-AGAATTCTATTACAATTTGAGCCATTCT-3′ (SEQ ID NO:10018)) encoding an EcoRI restriction site and the 3′ end of the murI gene were used to amplify the murI gene of  H. pylori  using genomic DNA prepared from the J99 strain of  H. pylori  as the template DNA for the PCR amplification reactions (Current Protocols in Molecular Biology, John Wiley and Sons, Inc. F. Ausubel et al., editors, 1994). To amplify a DNA sequence containing the murI gene, genomic DNA (25 nanograms) was introduced into each of two reaction vials containing 1.0 micromole of each synthetic oligonucleotide primer, 2.0 mM MgCl 2 ; 0.2 mM of each deoxynucleotide triphosphate (dATP, dGTP, dCTP &amp; dTTP), and 1.25 units of heat stable DNA polymerases (Amplitaq, Roche Molecular Systems, Inc., Branchburg, N.J., USA) in a final volume of 50 microliters. The following thermal cycling conditions were used to obtain amplified DNA products for the murI gene using a Perkin Elmer Cetus/GeneAmp PCR System 9600 thermal cycler:  
     [0625] Conditions for Amplification of  H. Pylori  MurI (SEQ ID NO: 7635);  
     [0626] Denaturation at 94° C. for 2 min,  
     [0627] 2 cycles at 94° C. for 15 sec, 30° C. for 30 sec and 72° C. for 15 sec  
     [0628] 23 cycles at 94° C. for 15 sec, 53° C. for 30 sec and 72° C. for 15 sec  
     [0629] Reactions were concluded at 72° C. for 20 minutes  
     [0630] Upon completion of thermal cycling reactions, the amplified DNA was washed and purified using the Qiaquick Spin PCR purification kit (Qiagen, Gaithersburg, Md., USA). The amplified DNA sample was subjected to digestion with the restriction endonucleases, NdeI and EcoRI (New England Biolabs, Beverly, Mass. USA) (Current Protocols in Molecular Biology, Ibid). The DNA samples from each of two reaction mixtures were pooled and subjected to electrophoresis on a 1.0% SeaPlaque (FMC BioProducts, Rockland, Me., USA) agarose gel. DNA was visualized by exposure to ethidium bromide and long wave uv irradiation. Amplified DNA encoding the  H. pylori  murI gene was isolated from agarose gel slices and purified using the Bio 101 GeneClean Kit protocol (Bio 101 Vista, Calif., USA).  
     [0631] Cloning of  H. Pylori  DNA Sequences into the pET-23 Prokaryotic Expression Vector.  
     [0632] The pET-23b vector can be propagated in any  E. coli  K-12 strain, e.g., HMS174, HB101, JM 109, DH5α, etc., for the purpose of cloning or plasmid preparation. Hosts for expression include  E. coli  strains containing a chromosomal copy of the gene for T7 RNA polymerase. These hosts are lysogens of bacteriophage DE3, a lambda derivative that carries the lacI gene, the lacUV5 promoter and the gene for T7 RNA polymerase. T7 RNA polymerase is induced by addition of isopropyl-B-D-thiogalactoside (IPTG), and the T7 RNA polymerase transcribes any target plasmid, such as pET-28b, carrying its gene of interest. Strains used in our laboratory include: BL21 (DE3) (Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W. (1990) Meth. Enzymol. 185, 60-89).  
     [0633] The pET-23b vector (Novagen, Inc., Madison, Wis., USA) was prepared for cloning by digestion with NdeI and EcoRI (Current Protocols in Molecular Biology, Ibid). Following digestion, the amplified, agarose gel-purified DNA fragment carrying the murI gene was cloned (Current Protocols in Molecular Biology, Ibid) into the previously digested pET-23b expression vector. Products of the ligation reaction were then used to transform the BL21 (DE3) strain of  E. coli.    
     [0634] Transformation of Competent Bacteria with Recombinant Plasmids  
     [0635] Competent bacteria,  E coli  strain BL21 or  E. coli  strain BL21(DE3), were transformed with recombinant pET23-murI expression plasmid carrying the cloned  H. pylori  sequence according to standard methods (Current Protocols in Molecular, Ibid). Briefly, 1 microliter of ligation reaction was mixed with 50 microliters of electrocompetent cells and subjected to a high voltage pulse, after which, samples were incubated in 0.45 milliliters SOC medium (0.5% yeast extract, 2.0% tryptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4  and 20 mM glucose) at 37° C. with shaking for 1 hour. Samples were then spread on LB agar plates containing 100 microgram/ml ampicillin for growth overnight. Transformed colonies of BL21 were then picked and analyzed to evaluate cloned inserts as described below.  
     [0636] Identification of Recombinant pET Expression Plasmids Carrying  H. Pylori  Sequences  
     [0637] Individual BL21 clones transformed with recombinant pET-23-murI were analyzed by PCR amplification of the cloned inserts using the same forward and reverse primers, specific for each  H. pylori  sequence, that were used in the original PCR amplification cloning reactions. Successful amplification verified the integration of the  H. pylori  sequences in the expression vector (Current Protocols in Molecular Biology, Ibid).  
     [0638] Isolation and Preparation of Plasmid DNA from BL21 Transformants Colonies carrying pET-23-murI vectors were picked and incubated in 5 mls of LB broth plus 100 microgram/ml ampicillin overnight. The following day plasmid DNA was isolated and purified using the Qiagen plasmid purification protocol (Qiagen Inc., Chatsworth, Calif., USA).  
     [0639] Cloning and Expression of the  E. Coli  groE Operon  
     [0640] It has been demonstrated that coexpression of the  E. coli  murI gene with the genes in the  E. coli  groE operon reduces the formation of insoluble inclusion bodies containing recombinant glutamate racemase (Ashiuchi, M., Yoshimura, T., Kitamura, T., Kawata, Y., Nagai, J., Gorlatov, S., Esaki, N. and Soda, K., 1995,  J. Biochem.  117, 495-498). The groE operon encodes two proteins, GroES (97 amino acids) and GroEL (548 amino acids), which are molecular chaperones. Molecular chaperones cooperate to assist the folding of new polypeptide chains (F. Ulrich Hartl, 1996, Nature London 381, pp. 571-580).  
     [0641] The 2210 bp DNA sequence encoding the groE operon of  E. coli  (NCBI Accession number X07850) was isolated by polymerase chain reaction (PCR) amplification cloning. A synthetic oligonucleotide primer (5′-GCGAATTCGATCAGAATTTTTTTTCT-3′ (SEQ ID NO:10019)) encoding an EcoRI restriction site and the 5′ terminus of the groE operon containing the endogenous promoter region of the groE operon and a primer (5′-ATAAGTACTTGTGAATCTTATACTAG-3′ (SEQ ID NO:10020)) encoding a ScaI restriction site and the 3′ end of the groEL gene contained in the groE operon were used to amplify the groE operon of  E. coli  using genomic DNA prepared from  E. coli  strain MG1655 as the template DNA for the PCR amplification reactions (Current Protocols in Molecular Biology, Ibid). To amplify a DNA sequence containing the  E. coli  groE operon, genomic DNA (12.5 nanograms) was introduced into each of two reaction vials containing 0.5 micromoles of each synthetic oligonucleotide primer, 1.5 mM MgCl 2 , 0.2 mM each deoxynucleotide triphosph te (dATP, dGTP, dCTP &amp; dTTP) and 2.6 units heat stable DNA polymerases (Expanded High Fidelity PCR System, Boehringer Mannheim, Indianapolis, Ind.) in a final volume of 50 microliters. The following thermal cycling conditions were used to obtain amplified DNA products for the groE operon using a Perkin Elmer Cetus/GeneAmp PCR System 9600 thermal cycler:  
     [0642] Conditions for amplification and cloning of the  E. coli  groE operon;  
     [0643] Denaturation at 94° C. for 2 min,  
     [0644] 2 cycles at 94° C. for 15 sec, 30° C. for 30 sec and 72° C. for 2 min  
     [0645] 23 cycles at 94 C for 15 sec, 55° C. for 30 sec and 72° C. for 2 min  
     [0646] Reactions were concluded at 72° C. for 8 minutes  
     [0647] Upon completion of thermal cycling reactions, the amplified DNA was washed and purified using the Qiaquick Spin PCR purification kit (Qiagen, Gaithersburg, Md., USA). The amplified DNA sample was subjected to digestion with the restriction endonucleases, EcoRI and ScaI New England Biolabs, Beverly, Mass. USA) (Current Protocols in Molecular Biology, Ibid). The DNAs from each of two reaction mixtures were pooled and subjected to electrophoresis in a 1.0% SeaPlaque (FMC BioProducts, Rockland, Me., USA) agarose gel. DNA was visualized by exposure to ethidium bromide and long wave uv irradiation. DNA contained in slices isolated from the agarsoe gel was purified using the Bio 101 GeneClean Kit protocol (Bio 101 Vista, Calif., USA).  
     [0648] A DNA fragment, EcoRI to ScaI, containing the  E coli  groE operon was cloned into the corresponding sites of the pACYC184 expression vector (New England Biolabs, Beverly, Mass., USA) to make pACYC184-groE. The BL21 (DE3) strain of  E. coli  was transformed with pACYC-groE. A tetracycline-resistant transformant overexpressing proteins of M r ˜14,000 (GroES) and M r ˜60,000 (GroEL) was isolated.  
     [0649] Transformation of  E. Coli  Strain BL21(DE3) Carrying the pACYC-groE plasmid of  E. coli.    
     [0650] Competent bacteria derived from a clone of strain BL21 (DE3) carrying the pACYC-groE plasmid were transformed with 50 nanograms of pET23-murI plasmid DNA, isolated as described above (Current Protocols in Molecular Biology, Ibid). A clone of BL21(DE3) carrying both the pACYC-groE expression plasmid and the pET-23-murI plasmid was isolated and used for expression of recombinant glutamate racemase as described below.  
     [0651] Expression of Recombiant  H. Pylori  murI (SEQ ID NO: 2873; SEQ ID NO: 7635)  
     [0652] A bacterial clone of BL21 (DE3) carrying both the pACYC-groE expression plasmid and the pET-23-murI plasmid was cultured in LB broth supplemented with 1.0 mM D,L-glutamic acid and 100 microgram/ml ampicillin and 10 micrograms/ml tetracycline at 30° C. until an optical density at 600 nM of 0.5 to 1.0 O.D. units was reached, at which point, isopropyl-beta-D-thiogalactoside (IPTG) was added to the culture at a final concentration of 1.0 mM. Cells were cultured overnight to induce gene expression of the  H. pylori  recombinant DNA constructions.  
     [0653] After induction of gene expression with IPTG, bacteria were pelleted by centrifugation in a Sorvall RC-3B centrifuge at 3000×g for 20 minutes at 4° C. Pellets were resuspended in 50 milliliters of cold 10 mM Tris-HCl, pH 8.0, 0.1 M NaCl and 0.1 mM EDTA (STE buffer). Cells were then centrifuged at 2000×g for 20 min at 4° C. Pellets were weighed (average wet weight =6 grams/liter) and processed to purify recombinant protein as described below.  
     [0654] Purification of Soluble Glutamate Racemase  
     [0655] All steps were carried out at 4° C. Cells were suspended in 4 volumes of lysis buffer (50 mM Potassium phosphate, pH 7.0, 100 mM NaCl, 2 mM EDTA, 2 mM EGTA, 10% glycerol, 10 mM D,L-glutamic acid, 0.1% β-mercaptoethanol, 200 μg/ml lysozyme, 1 mM PMSF, and 10 ug/ml each of leupeptin, aprotinin, pepstatin, L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone (TLCK), L-1-chloro-3-[4-tosylamido]-4-phenyl-2-butanone (TPCK), and soybean trypsin inhibitor, and ruptured by three passages through a small volume microfluidizer (Model M-110S, Microfluidics International Corporation, Newton, Mass.). The resultant homogenate was diluted with 1 volume of buffer A (10 mM Tris-HCl pH 7.0, 0.1 mM EGTA, 10% glycerol, 1 mM DL-Glutamic acid, 1 mM PMSF, 0.1% beta-mercaptoethanol), made 0.1% Brij-35, and centrifuged (100,000×g, 1 h) to yield a clear supernatant (crude extract).  
     [0656] After filtation through a 0.80-um filter, the extract was loaded directly onto a 20 ml Q-Sepharose column pre-equilibrated in buffer A containing 100 mM NaCl and 0.02% Brij-35. The column was washed with 100 ml (5 bed volumes) of Buffer A containing 100 mM NaCl and 0.02% Brij-35, then developed with a 100-ml linear gradient of increasing NaCl (from 100 to 500 mM) in Buffer A. A band of M r =28,000 corresponding to glutamate racemase, the product of the recombinant  H. pylori  murI gene, eluted at a gradient concentration of approximately 200-280 mM NaCl. Individual column fractions were then characterized for glutamate racemase activity (see below for description of assay) and the protein profile of the fractions were analyzed on 12% acrylamide SDS-PAGE gels.  
     [0657] Fractions containing glutamate racemase were pooled, brought to 70% saturation with solid (NH 4 ) 2 SO 4 , stirred for 20 min, and then centrifuged at 27,000×g for 20 min. The resulting pellet was resuspended in lysis buffer to a final volume of 8 ml and loaded directly onto a 350-ml column (2.2×92 cm) of Sephacryl S-100HR gel filtration medium equilibrated in buffer B (10 mM Hepes pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 10% glycerol, 1 mM D,L-glutamatic acid, 0.1 mM PMSF, 0.1% beta-mercaptoethanol) and run at 30 ml/h. Fractions found to contain a glutamate racemase activity were pooled, and 0.5 volume of buffer C (10 mM Tris pH 7.5, 0.1 mM EGTA, 10% glycerol, 1 mM D,L-glutamic acid, 0.1 mM PMSF, 0.1% B-mercaptoethanol) was added (to reduce the NaCl concentration to 100 mM), and loaded onto a MonoQ 10/10 high-pressure liquid chromatography column equilibrated in buffer C containing 100 mM NaCl. The column was washed with 5 bed volumes of this buffer and developed with a 40 ml linear gradient of increasing NaCl (from 100 to 500). Glutamate racemase eluted as a sharp peak at 310 mM NaCl. Fractions containing a glutamate racemase activity were pooled, concentrated by dialysis against storage buffer [50% glycerol, 10 mM 3-(N-morpholino-propanesulfonic acid (MOPS) pH 7.0, 150 mM NaCl, 0.1 mM EGTA, 0.02% Brij-35, 1 mM dithiothreitol (DTT)], and stored at −20° C.  
     [0658] Assays for Glutamate Racemase Activity.  
     [0659] Conversion of D-glutamate to L-glutamate (Two Enzyme Coupled Assay)  
     [0660] The activity of glutamate racemase, interconversion of the enantiomers of glutamic acid, was measured using D-glutamic acid as substrate. The method of Gallo and Knowles (Gallo, K. A. and Knowles, J. R., 1993, Biochmistry 32, 3981-3990) that was used to measure the glutamate racemase activity of  Lactobacillus fermenti  was adapted for the measurement of glutamate racemase activity of the  H. pylori  murI gene product isolated as a recombinant protein from  E. coli . In this assay, the measurement of the activity of glutamate racemase is linked to an OD change in the visible range in a series of coupled reactions to the activities of L-glutamate dehydrogenase (reduction of NAD to NADH) and diaphorase (reduction of the dye p-iodonitrotetrazolium violet, INT). Initial rates were determined by following the increase in absorbance at 500 nm in a reaction volume of 200 μl containing 50 mM Tris-HCl, pH 7.8, 4% v/v glycerol, 10 mM NAD, 2 mM INT, 60 Units/ml L-glutamate dehydrogenase, 5 Units/ml diaphorase, and varying concentrations of either substrate (from 0.063 mM to 250 mM D-glutamic acid) or purified enzyme (from 1 μg to 50 μg). After a preincubation of all reagents except either the substrate (D-glutamic acid) or the enzyme (murI gene product) for a period of 5 minutes, reactions were initiated by adding the missing ingredient (i.e., the enzyme or the subtrate, as required), and the increase in optical density at 500 nm was measured in a Microplate Spectophotometer System (Molecular Devices, Spectra MAX 250). Measurements were followed for 20 minutes, and initial velocities were derived by calculating the maximum slope for the absorbance increases. The coupled reaction can be summarized as shown below:  
                 
 
     [0661] Conversion of D-glutamate to L-glutamate (Single Enzyme Coupled Assay)  
     [0662] In this assay, the conversion of D-glutamic acid to L-glutamic acid is coupled to the conversion of L-glutamic acid and NAD + by L-glutamate dehydrogenase to 2-oxoglutarate, ammonia. The production of NADH is measured as an increase of absorbance at 340 nm (the reduction of NAD +  to NADH) at 37° C. The standard assay mixture (adapted from Choi, S- Y,, Esaki, N., Yoshimura, T., and Soda, K., 1991, Protein Expression and Purification 2, 90-93) contained 10 mM Tris-HCl, pH 7.5, 5 mM NAD+, 5 Units/ml L-glutamate dehydrogenase, varying concentrations of the substrate D-Glutamic Acid (0.063 mM to 250 mM), and the purified recombinant  H. pylori  enzyme glutamate racemase (1 μg to 50 μg). The reaction was started by the addition of either the substrate D-glutamic acid or the recombinant glutamate racemase after a preincubation at 37° C. for 5 minutes with all of the other assay ingredients. The change in absorbance at 340 nm was measured in a Spectra MAX 250. Initial velocities were derived from the initial slopes. The coupled reactions can be summarized as shown below:  
                 
 
     [0663] Results  
     [0664] 1) Expression of the  H. Pylori  murI gene in  E. Coli  Cells  
     [0665] To examine its biochemical properties, the  H. pylori  glutamate racemase was overexpressed in  E. coli  and purified. In the presence of the  E. coli  chaperones GroES and GroEL, the glutamate racemase was expressed as a soluble protein. About 20 mg of soluble MurI (SEQ ID NO: 2873; SEQ ID NO: 7635) was produced per liter of culture as judged by intensity of the protein band after SDS-PAGE. No band corresponding to the molecular weight of murI protein was seen in control gel lanes containing extracts from cells transformed with the pET vector lacking a murI insert. Addition of 1 mM DL-glutamic acid during cultivation of the expressing cells increased the apparent expression level by about five-fold.  
     [0666] 2) Purification of Recombinant  H. Pylori  murI Protein  
     [0667] MurI was purified by cation exchange chromatography and gel filtration. Upon SDS-PAGE analysis, the purified protein migrated as a single polypeptide species with an apparent mass 29 kDa which is consistent with the predicted mass of 28,858.  
     [0668] 3) Kinetic Properties of Recombinant  H. Pylroi  murI enzyme  
     [0669] Kinetic constant for recombinant glutamate racemase were estimated by assaying its activity at various concentrations of protein and D-glutamic acid as described above. Purified recombinant  H. pylori  glutamate racemase exhibits a V max  of ˜300 nmoles/min/mg protein (kcat=8.6 min-1) and a K m  of ˜100 μM for D-glutamate. Although the Vmax value is lower than that observed for highly purified glutamate racemase from some other bacterial species, its Km for D-glutamic acid is higher than that observed for the enzyme from most other species, resulting in a catalytic efficiency (k cat /K m ) which is typical of purified preparation from  E. coli  and  P. Pentococcus.    
     [0670] 4) Characterization of MurI: Inhibition by L-serine-O Sulfate  
     [0671] The  H. pylori  glutamate racemase was tested for inactivation with a sucuide inhibitor, L-serine-O sulfate, which is known to inhibit murI from  E. coli . The enzyme was incubated in the presence of 20 mM L-serine-O sulfate, and at different times interval, aliquots were removed to determine residual activity. The initial velocity of purified recombinant  H. pylroi  murI protein was determined in the single enzyme coupled asssay following incubation with the inhibitor L-serine-O-sulfate (LSOS) at 20 mM for the times indicated on the x-axis. The control was incubated in an identical manner but without LSOS. As shown in FIG. 7, the  H. pylori  glutamate racemase can be readily inactivated by the inhibitor.  
     [0672] Future Application of the Glutamate Racemase Activity in High Throughput Drug Screening Assays.  
     [0673] The assays for measurement of  H. pylori  glutamate racemase activity described above have been carried out in 96-well plates in which multiple reactions were conducted simultaneously. Measurements of activity in a multi-well format are readily amenable to scale-up to permit rapid analysis of numerous compounds for inhibition of the glutamate racemase activity. Compunds which inhibit the activity of glutamate racemase may have application as novel antibiotics and may be suitable for the treatment and eradication of bacterial (e.g.,  H. pylori ) infections in humans. Known inhibitors of glutamate racemase, such as L-serine-O-sulfate, can be used to calibrate high throughput screens of new compound libraries to facilitate identification of new compounds with properties suitable for in vivo human therapeutics.  
     [0674] IX. Cloning Purification, and Characterization of the Gene Encoding  H. Pylori  MurC (SEQ ID NO: 2845; SEQ ID NO: 7607).  
     [0675] Background on UDP-N-acetylmuramyl-alanine Synthetase (MurC)  
     [0676] MurC catalyzes peptide bond formation between the lactyl ether of UDP-N-acetylmuramate and L-alanine to form UDP-N-acetylmuramyl-alanine in one of the initial steps of bacterial peptidoglycan biosynthesis. The enzyme couples the formation of an amide bond with the cleavage of ATP to ADP and inorganic phosphate.  
                 
 
     [0677] Peptidoglycan biosynthesis is both essential and unique to bacteria. A variety of bactericidal agents exist which interrupt peptidoglycan biosynthesis (e.g., lactams, vancomycin, phosphonomycin, D-cycloserine, alafosfalin, halovinylglycines, liposydomycin B). These compounds act at various steps in the pathway and all cause the induction of cell lysis. MurC is one of two cell wall enzymes (the other is the alanine racemase) which are thought to be inhibited by the cell wall active agent alafosfalin.  
     [0678] The assembly of the peptide moiety of the peptidoglycan monomer unit in  E. coli  is carried out by the stepwise addition of L-alanine, D-glutamate, diaminopimelate and D-alanine-D-alanine onto UDP-N-acetylmuramate catalyzed by four adding enzymes (MurC, MurD, MurE and MurF, respectively). The  H. pylori  genome contains a 475 amino acid open reading frame with relatively high homology to MurC proteins from  H. influenzae  and  E. coli  with lower similarity to that from  P. gingivalis.  Alignment of  H. pylori  MurC with these homologs reveals an overall identity of 14%.  
     [0679] Overproduction and Purification of Functional  H. Pylori  MurC (SEQ ID NO: 7607)  
     [0680] The  H. pylori  enzyme has been successfully overproduced in  E coli  and purified to yield functional enzyme of the appropriate molecular weight as evidenced by SDS-PAGE. Assay of the MurC protein relies on enzymatic synthesis of the substrate, UDP-N-acetylmuramate which is accessible by linking the two preceding enzymes in the peptidoglycan biosynthetic pathway. That procedure involves the incubation of purified  E. coli  MurA and MurB with commercially available UDP-N-acetylglucosamine and phosphoenolpyruvate followed by reverse phase HPLC purification of the product UDP-N-acetylmuramic acid. Assay of the UDP-N-acetylmuramate synthetase activity of the MurC protein is possible through any of the three methodologies: (i) substrates and products can be monitored by reverse phase HPLC; (ii) ADP production can be monitored continuously through coupled assay with pyruvate kinase and lactate dehydrogenase by following the stoichiometric oxidation of NADH; and (iii) inorganic phosphate produced in the reaction can be monitored spectrophotometrically by complex with molybdate in the presence of the dye malachite green. The inorganic phosphate assay will be most amenable to high throughput screening against compound libraries.  
     [0681] Cloning and Over-expression of  H. Pylori  MurC (SEQ ID NO: 7607)  
     [0682] Two primers were designed for the PCR cloning of murC, MURCE5 GCCATATGCTTGAAACCCCAAAAGTTTTACTCAAAAACC (SEQ ID NO:10021)) and MURCE3 GCGAATTCGCTCGCTCCTATAATCCCTACG (SEQ ID NO:10022)). The 5′ primer was designed incorporating an NdeI site, and the 3′ primer was designed incorporating an EcoRI site. These restriction sites were chosen to allow for the cloning of the entire murC gene into the expression vectors pET-23a and pET-28b, the latter vector allowing for the incorporation of a His tag into the over-expressed recombinant protein to aid in the purification process. Initial stability problems were experienced when attempts were made to clone the murC gene into a high copy number vector, therefore, the murC PCR product was blunt end cloned into the EcoRV site of the low copy number pOK12 plasmid prior to insertion into the expression vectors. The murC gene was excised from pOK12 using NdeI and EcoRI, and ligated into the compatible sites within pET-23a and pET-28b. The complete murC gene was then sequenced to ensure that no aberrant mutation had occurred during the PCR process, and that the primary amino acid sequence of the isolated recombinant protein was identical to the native protein.  
     [0683] Purification and Assay for  H. Pylori  MurC (SEQ ID NO: 7607)  
     [0684] Approximately 15 mg of His-tagged MurC was purified to homogeneity from a six liter fermentation of  E coli  strain BL21 DE3/pET28b-HpMurC according to the following procedure. Freshly transformed cells (6L) were grown to an optical density (600 nm) of 0.5 in Luria-Bertani media. These cells were then induced with 1 mM isopropylthiogalactoside, allowed to grow for another 3 hours and harvested by centrifugation. Cells were resuspended in 75 ml of 100 mM Tris·HCl, pH 8, 2.5 mM 2-mercaptoethanol and 10% (vol/vol) glycerol. To that mixture, lysozyme was added to a final concentration of 0.2 mg/ml and allowed to incubate for 10 min at 4° C. with gentle mixing before two passages at 2000 psi through a French Press. Cell debris and membranes were then pelleted by centrifugation at 200,000 g for 60 min. The resulting supernatant was applied to a Pharmacia HiTrap chelating nickel-charged column (10 ml) and washed with 5 column volumes of 20 mM sodium phosphate, 0.5 M NaCl, 10 mM imidazole, pH 7.5. The nickel column was then eluted with 500 mM imidizole in the above buffer. The nickel column eluate was subsequently dialyzed overnight against 50 mM Tris·HCl, 2.5 mM 2-mercaptoethanol, 10% glycerol, pH 8.0. The dialysate was then loaded onto a 15 ml Resource Q (Pharmacia) anion exchange column equilibrated in 50 mM Tris·HCl, 2.5 mM 2-mercaptoethanol, 10% glycerol, pH 8.0 and washed with ten column volumes of the same buffer. The column was then subjected to a 120 ml gradient from 0 to 500 mM KCl. MurC eluted with about 150 mM KCl and a total volume of 22 ml was quick-frozen with liquid nitrogen in 0.5 ml aliquots for subsequent assay.  
     [0685] UDP-N-acetylmurmate was synthesized enzymatically with partially purified  E. coli  MurA and MurB from overproducing strains described previously (Benson et al., 1993 , Biochemistry  32:2024; Brown et al., 1994, Biochemistry 33:10638). The enzymatic synthesis involved purification of a 1 hour incubation of 0.5 mg MurA, 0.5 mg MurB, 20 mM UDP-GlcNAc, 20 mM PEP, 10 mM KCl; 20 mM NADPH, 5 mM DTT, 100 mM Tris·HCl, pH 8. UDP-N-acetylmurmate was purified from the mixture by isocratic reverse phase HPLC in 50 mM ammonium formate buffer pH 3.7 on a 10×250 mm, 5 μm C-18 column from YMC (Japan).  
     [0686] Purified  H. pylori  MurC was assayed using a continuous assay by coupling ADP formation with NADH oxidation by the pyruvate kinase/lactate dehydrogenase enzyme couple. Assay conditions were as follows: 100 mM Tris·HCl pH 8.0, 2.5 mM 2-mercaptoethanol, 20 mM KCl, 10 mM DTT, 2 mM MgCl 2 , 10 mM PEP, 0.15 mM NADH, 3.5 units of pyruvate kinase, 5.5 units of lactate dehydrogenase (Sigma), 500 μM UDP-N-acetylmuramate, 1 mM L-alanine, 1 mM ATP and 0.0018 mg/ml MurC at 37° C. Under these conditions  H. pylori  MurC exhibited a specific activity of 5900 nmol/min/mg. In this assay ADP production was shown to be dependent on UDP-N-acetylmuramate, L-alanine and enzyme. Previously boiled enzyme could not support ADP formation. Steady state analysis of the dependence of reaction velocity on substrate concentration was done by varying the concentration of a single substrate in concentrations ranging from 0.5 to 5 times K m  while holding the other two substrates constant at 5 times K m  concentrations. Apparent K m &#39;s for L-alanine, ATP and UDP-N-acetylmuramate were 80, 100 and 201M, respectively.  
     [0687] Based on the above, high throughput drug screening assays which measure the UDP-N-acetylmuramyl-alanine synthetase activity can be performed. The assays for the measurement of the  H. pylori  MurC activity can be carried out in a 96-well plate amenable for HTS. Such an assay will result in hits that can then be developed into drugs by those skilled in the art.  
     [0688] X. Expression Partial Purification and Enzymatic Measurement of the  H. Pylori  3-deoxy-D-manno-2-octulosonate-8-phosphate Synthase.  
     [0689] Background on 3-deoxy-D-manno-2-octulosonic Acid (KDO)  
     [0690] 3-deoxy-D-manno-2-octulosonic acid (KDO) is an unusual 8-carbon sugar found in the lipopolysaccharides of a wide variety of Gram-negative bacteria. It plays a crucial role in the assembly process of lipopolysaccharides providing a link between lipid A and the growing polysaccharide chain (Inouye, 1979). It has been shown that an interruption of the production and utilization of KDO leads to a buildup of lypopolysaccharide precursors and growth inhibition (Ghalambor et al., 1966; Mauson et al., 1978). Since KDO is-a molecule found only in Gram-negative organisms and is required for lipopolysacchirides synthesis and cell growth, the inhibition of one of the enzyme(s) responsible for the biosynthesis of KDO represent an attractive target for the discovery of a novel class of antibiotics directed against  Helicobacter pylori  infections.  
     [0691] There are at least four enzymes involved in the synthesis and utilization of KDO. The actual precursor is its 8-phosphate (KDO-8-P), which is formed by the unusual condensation of D-arabinose 5-phosphate (Ara5P) and phosphoenolpyruvate (PEP), catalyzed by the KDO-8-P synthase (EC 4.1.2.16). The available evidence supports the following overall reaction for KDO-8-P formation:  
       Ara 5 P+PEP KDO -8- P+Pi    
     [0692] KDO-8-P is then dephosphorylated by the next enzyme in the pathway to give KDO which is subsequently activated to cytidine-5-monophosphate-KDO and transferred to a lipopolysaccharide percursor.  
     [0693] Expression and Analysis of Soluble KDO-8-P Synthase (SEQ ID NO: 6945)  
     [0694] Recombinant  H. pylori  KDO-8-P synthase was expressed in  Escherichia coli  using the pET28b vector, that carries the strong T7 expression system, to express a histidine-tagged fusion protein. We have investigated the expression of soluble material at 25, 30 and 37° C. Cells were grown to an OD 600  of 0.6 in LB broth supplemented with 30 ug/ml kanamycin and 10 mM Potassium Phosphate pH 7.0, at which point, β-D-thiogalactopyranoside (IPTG) was added to a final concentration of mM. Cells were induced for 16 hours for 25′-C, 4 hours for 30° C., and 3 hours for 37° C. Cultures were harvested by centrifugation (20 min. at 3000×g, 4-C), washed with STE buffer (10 mM tris(hydroxymethyl)aminimethane (Tris)-HCl pH 8.0, 100 mM NaCl, 1 mM EDTA), and the cell pellets were stored at −70° C. A 1 litre culture of bacteria typically yielded 2 to 3 g (wet weight) of cells.  
     [0695] Analysis of Cell Fractionation  
     [0696] All steps were carried out at 4° C. Frozen cells were thawed, resuspended in five volumes of lysis buffer (50 mM Phosphate, pH 8, 0.5 M NaCl, 5 mM imidazole) with 10% glycerol, 10 mM PEP, 0.1% β-mercaptoethanol, 1 mM phenylmethylsulfonyl fluoride (PMSF), and 10 ug/ml each of leupeptin, aprotinin, pepstatin, L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone (TLCK), L-1-chloro-3-[4-tosylamido]-4-phenyl-2 butane (TPCK), and soybean trypsin inhibitor (Boehringer-Mannhein), and ruptured by several passages through a small volume microfluidizer (Model M-11OS, Microfluidics International Corporation, Newton, Mass.). Brij 35 was added to the resultant homogenate to a concentration of 0.1%, and the extract was centrifuged (100,000×g for 1 hour) to yield a clear supernatant (crude extract). The protein concentrations of both the soluble fraction and the cell pellet fraction were measured according to the method of Bradford (1976) and 15-g of soluble fractions and 4-g of cell pellets were analyzed by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis.  
     [0697] Expression of soluble recombinant KDO-8-P synthase was similar in all temperatures studied. Note that the insoluble fractions were overloaded relative to the soluble fraction (about 7-fold), therefore the amount of insoluble KDO-8-P synthase may be an overestimate relative to the soluble fractions.  
     [0698] Purification  
     [0699] Cell fractions containing recombinant  H. pylori  KDO-8-P synthase were filtered through a 0.45-m filter, the soluble fraction was loaded directly onto a 5-ml Ni 2+  nitrolotriacetate-agarose (HiTrap Chelating, Pharmacia) (Hochuli et al. 1987) pre-equilibrated in lysis buffer containing 10% glycerol, 0.1% Brij 35 and 1 mM PMSF. The column was washed with 100 ml (20 bed volumes) of lysis buffer containing 10% glycerol, 0.1% Brij 35, and developed with a 60 ml linear gradient from 5 mM to 500 mM imidazole in lysis buffer containing 10% glycerol, 0.1% Brij 35 and 1 mM PMSF. Fractions were monitored by absorbance at OD280 nm, and peak fractions were analyzed by SDS-PAGE gel electrophoresis. Fractions containing the recombinant protein eluted at a concentration of 125 mM imidazole.  
     [0700] Assay 1  
     [0701] The enzyme reaction was performed in a final volume of 4001 containing 0.3 mM Ara5P, 0.3 mM PEP and 0.1 M Tris-acetate pH 7.5. Reactions were started with 10 g of purified enzyme and were followed spectrophotometrically at 232 nm for 15 minutes. As a control, the mixture minus enzyme was measured. No decrease in the absorbance at 232 nm was observed when no enzyme was added. Addition of 10 g KDO-8-P synthase resulted in a decrease of the absorbance at 232 nm, representing the utilization of PEP by the enzyme. An extinction coefficient of 2840 M cm −1  of PEP, was used to estimate that at room temperature, the activity of the enzyme was 0.7-mole/min/mg protein.  
     [0702] Assay 2  
     [0703] Because of the potential interference of test compounds on the assay at 232 nm, a method was developed to measure the amount of Pi released in the reaction (Lanzatta et al. 1979).  
     [0704] This assay ws performed in a 96 well ELISA place, 50 ul assay mixtures containing 0,1 M Tris-acetate (pH 7.5), 0.3 mM Ara5P and 0.3 mM PEP, and 1.28 ug/ml of KDO-8-P synthase were incubated at 25° C. for different times. The reaction was then quenched with the addition of the molybdate-malachite green reagent. As shown in FIG. 8, the reaction proceeded linearly for 60 minutes. Under those conditions, the activity measured was 0.734-moles/min/mg protein, which was similar to the rate that was measured with the 232 nm assay.  
     [0705] Stimulation of the Reaction with Brij 35  
     [0706] When assayed in the presence of 0.02% Brij 35, the reaction proceeded faster. Under those conditions, the activity increased up to 1 mole/min mg protein.  
     [0707] Stability of the Enzyme  
     [0708] The long term storage stability of the enzyme was tested over several conditions. When the enzyme was present in a buffer containing 50 mM phosphate pH 8.0 and 1 mM PEP, the enzyme could be kept at −20° C. with no loss of activity for at least three weeks. Enzyme stored in 50% glycerol appeared to have less activity during extended storage at −20° C. (FIG. 9).  
     [0709] XI. Over-production and Purification of Enzymatically Active  H. Pylori  Asd.  
     [0710] The asd enzyme of  H. pylori  has been over-produced successfully in  E. coli  and purified in sufficient quantities to allow assay development for high through-put screening. The purified protein was determined to be Asd by monitoring its migration of SDS-PAGE gels to determine its molecular mass and by determining the enzymatic activity of the protein.  
     [0711] Cloning of the  H. Pylori  and  E. Coli  asd Genes.  
     [0712] The open reading frame encoding Asd (SEQ ID NO: 1783; SEQ ID NO: 6545) was identified from the genomic sequence of  H. pylori  by its coding sequence homology to the Asd enzyme of a number of other bacteria. Two oligonucleotide primers were designed to allow the amplification by PCR of the  H. pylori  asd gene from total  H. pylori  chromosomal DNA. The Hpasd1 primer 5′-AAACATATGAAGACTTATAATGTCGCTATTG-3′ (SEQ ID NO: 10023) was designed to contain an Nde 1 restriction site to facilitate cloning of the product into expression vectors. For the same reason, the Hpasd3 primer 5′-TTTGGATCCTTTAAGCAAGCTCAAGCGTTC-3′ (SEQ ID NO: 10024) contained a restriction site for Bam HI. The expression vectors chosen for expression were pET28b and pET30a. pET28b allows the addition of a His-tag to the N-terminus of Asd to facilitate purification.  
     [0713] The asd gene was amplified by PCR using the following conditions;  
     [0714] Initial denaturation at 94° C. for 5 min followed by 30 cycles of  
     [0715] Denaturation at 94° C. for 30 sec.  
     [0716] Annealing at 50° C. for 30 sec  
     [0717] Extension at 72° C. for 1 min  
     [0718] A final extension period of 7 min at 72° C. was also performed.  
     [0719] The product of the PCR was purified using the QIAEX II gel purification system (Qiagen, Chatsworth Calif.) and ligated overnight at 16° C. to the cloning vector pGEM-T (Promega, Madison Wis.) and transformed into  E. coli  DH5α. Resulting colonies were screened for the presence of the appropriate insert by restriction digest analysis. One clone containing the inserted asd gene was selected, digested with Nde I and Bam HI and run on an agarose gel. The band corresponding to the asd gene was cut from the gel, purified with the QIAEX II kit (Qiagen ibid.) and ligated overnight at 16° C. to either pET28b or pET30a. Clones containing the asd gene were identified by a white colony appearance on agar plates containing X-Gal. Appropriate constructs were confirmed by restriction digest analysis and by DNA sequencing of the entire insert.  
     [0720] The asd gene of  E. coli  was amplified using primers Ecasd35′-AAAGGTACCATGAAAAATG TTGGTTTTATCGGC-3′ (SEQ ID NO: 10025) and Ecasd25′-CCAGAATTCATGAATAAAGATTACGCCAG-3′ (SEQ ID NO: 10026) with total  E. coli  chromosomal DNA as a template. The PCR conditions were as described above for the  H. pylori  asd gene. The Ecasd1 primer was designed to contain a Kpn I restriction site to facilitate cloning of the product into expression vectors. For the same reason, the Ecasd2 primer contained the restriction site for EcoRI. The amplified band containing  E. coli  asd was isolated from the gel, purified with QIAEX (ibid.), ligated into pGEM-T and transformed into  E. coli  DH5α. Colonies were screened for the presence of the appropriate insert by restriction digest analysis. One clone containing the inserted asd gene was selected, digested with Kpn I and Eco RI and run on an agarose gel. The band corresponding to the  E. coli  asd gene was cut from the gel, purified with the QIAEX II kit (Qiagen, ibid.) and ligated overnight at 16° C. to pET28b.  
     [0721] Expression of  E. Coli  and  H. Pylori  Asd  
     [0722] The pET28b plasmid containing either  E. coli  or  H. pylori  asd (SEQ ID NO: 1783; SEQ ID NO: 6545) was transformed into  E. coli  HMS174 pLysS for expression of the protein. Cells were grown in LB medium supplemented with 50 mg/ml kanamycin (Fisher, Pittsburgh Pa.). Growth was monitored by optical density (OD) at 600 nm. When the OD reached 0.5 units, iso-propyl-thio-β-D-galactoside (IPTG)(Sigma, St. Louis Mo.) was added to a final concentration of 1 mM to induce expression of Asd. Induction was continued for 3 hr, followed by harvesting of the cells by centrifugation and storage of the cell pellet at −20° C.  
     [0723] The Asd enzyme was purified from the cell pellets using a nickel affinity column. Approximately 30 mg of His-tagged protein was purified to homogeneity from a pellet from a 330 ml culture according to the following procedure. The cell pellet was resuspended in 20 ml of PO 4  buffer (0.054 g Na 2 HPO 4.7 H 2 O, 0.028 g NaH 2 PO 4 .H 2 O, 0.59 g NaCL, pH 7.4) containing 0.1% (w/v) Triton X 100 and 100 ng/μl of lysozyme and incubated at 25° C. for 25 min. The mixture was sonicated twice for 20 sec on ice after which it was centrifuged at 10,000×g for 10 min at 4° C. The supernatant was then passed through a 45 μm filter and run through a 5 ml HiTrap chelating nickel-charged column (Pharmacia, Pascataway N.J.) and washed with 5 column volumes of 20 mM sodium phosphate, pH 7.5. The nickel column was then eluted with 300 mM imidazole in PO 4  buffer. The nickel column eluate was subsequently dialyzed overnight against 100 mM Tris.HCl, pH 7.5. Glycerol was added to a final concentration of 25% (w/v) was added to the purified protein which was then stored at −20° C.  
     [0724] Development of an Assay for High-throughput Screening of Asd Enzymatic Characterization of  H. Pylori  Aspartate Semialdehyde Dehydrogenase (Asd)  
     [0725] The enzymatic activity of purified His-tagged  H. pylori  Asd (SEQ ID NO: 6545) was characterized by assaying the reverse reaction of phosphorylation of L-aspartate-β-semialdehyde (ASA) to L-p-aspartyl phosphate, with concomitant reduction of NADP +  to NADPH. The formation of NADPH was detected by its absorbance, 340 nm, or by its fluorescence, excitation =340 nm, emission 455 nm.  
     [0726] ASA was synthesized enzymatically by the phosphorylation of L-aspartate to L-β-aspartyl phosphate by aspartokinase (LysC) coupled to the reduction of L-β-aspartyl phosphate to L-aspartate-α-semialdehyde by Asd. The procedure involved a 2 h preincubation of 0.3 mg of purified  E. coli  LysC and  E. coli  Asd with 2 mM aspartate, 1 mM NADPH, 10 mM DTT, 30 mM ATP, 30 mM MgSO 4 , 3 mM NH 4 OH and 100 mM Tris-HCl buffer, pH 8, followed by adsorption of the enzymatic product on a column of the cation exchange resin Dowex 50 (hydrogen form, 400 mesh). The resin was washed with 5× columns of water and the aldehyde eluted in 5 ml fractions (1 ml/min) with 4 N HCL. Presence of the aldehyde was confirmed using the Asd enzymatic assay in the reverse direction and absence of aspartyl phosphate by the Asd enzymatic assay in the forward direction (Black et al., 1955,  J. Biol. Chem.  213,39-50., Karsten et al., 1991,  Biochim. Biophys. Acta  1077, 209-219). The identity of the compound was also confirmed by  13 C and H +  NMR.  
     [0727] ASA in 4 N HCl was prepared daily for use in Asd assays by partial neutralization with addition of 1.0 equivalent of Na 2 HCO3 2− , with a resulting pH=1-2. The amount of ASA was determined enzymatically by complete reaction in the presence of excess NADP + and phosphate. ASA was found to be stable for up to 48 hours at pH 1.4, but is somewhat unstable at pH 7.7 (under assay conditions) with a t½=5 hrs. These observations are consistent with previous reports on the stability of ASA (Karsten et al., 1991 , Biochim. Biophys. Acta  1077, 209-219). Assays were performed in the following buffer: 200 mM HEPES, 0.3 mM NADP + , 30 mM NaH 2 PO 4 , 1.0 mM DTT, pH 7.5 or pH 8.0. ASA was used in the assay at concentrations of 5-165 μM by diluting the partially-neutralized ASA stock into the assay 10-200 fold, leading to a decrease in pH of 0.1 units or less. Reaction was initiated with the addition of enzyme to a final concentration of 3.5-70 nM.  
     [0728] Using  E. coli  Asd, the K m  for ASA and phosphate were found to be 104 μM and 1.3 mM, respectively, with a k cat =26 sec −1  at pH 7.5. These values are roughly consistent with those reported by Karsten and Viola (1991,  Biochim. Biophys. Acta  1077, 209-219) for the  E. coli  enzyme, considering the pH dependence of enzyme activity that was reported. For  H. pylori  Asd, we determined the K m  for ASA and phosphate to be 54 μM and 2.1 mM, respectively, with a k cat =5.4 sec −1  at pH 8.0. Thus, the  H. pylori  enzyme is similar to the  E. coli  enzyme with the exception of a decreased k cat  by about 5-fold. The pH dependence of  H. pylori  Asd activity, as measured by initial velocity, was found to be quite similar to that reported for the  E. coli  enzyme (Karsten et al., 1991,  Biochim. Biophys. Acta  1077, 209-219), with a pH optimum between 8.5 and 9.0. The activity of  H. pylori  Asd was found to be unaffected by ionic strength as high as 0.7 M.  
     [0729] Based on the above characterization, high throughput drug screening assays can be performed in a 96-well plate format using a fluorescence microplate reader, detecting NADPH formation by measuring an increase in fluorescence with excitation filter =355 nm, emission filter =460 nm. Using this format with 160 μM ASA and 20-50 nM  H. pylori  Asd in assay buffer at pH 8.0, a simple 30 to 60 minute assay can be utilized with excellent reproducibility and high signal to noise.  
     [0730] The above described assays (Examples X and XI) could be further developed for screening chemical compounds for activity and the subsequent identification of a drug active against the target and cause inhibition of a bacterium, preferably  H. pylori.    
     [0731] XII. Truncated Gene Expression and Protein Production  
     [0732] Identification, Cloning and Expression of Recombinant  Helicobacter Pylori  Sequences.  
     [0733] To facilitate the cloning, expression and purification of membrane proteins from  H. pylori , the pET gene expression system (Novagen), for cloning and expression of recombinant proteins in  Escherichia coli  was selected. Further, for proteins that have a signal sequence at their amino-terminal end, a DNA sequence encoding a peptide tag (His-tag) was fused to the 5′ end of the  H. pylori  DNA sequences of interest in order to facilitate purification of the recombinant protein products. In some cases, the DNA sequence was cloned in frame with the glutathione-S-transferase protein to produce a GST-fusion protein. The vectors used in this case were the pGEX series from Pharmacia LKB (Uppsala, Sweden).  
     [0734] PCR Amplification and Cloning of DNA Sequences Containing ORFs for Membrane and Secreted Proteins from the J99 Strain of  Helicobacter pylori.    
     [0735] The sequences chosen (from the list of the DNA sequences of the invention) for cloning from  H. pylori  strain J99 were prepared for amplification cloning by the polymerase chain reaction (PCR). Synthetic oligonucleotide primers for the ORF of interest (Table 15) specific for the predicted mature 5′ end of the ORF and either downstream (3′) of the predicted translational termination codon or at specific points within the coding region were designed and purchased (GibcoBRL Life Technologies, Gaithersburg, Md., USA). All forward primers (specific for the 5′ terminus of the region of ORF of interest) were designed to include either a BamHI or a NdeI restriction site. These primers within the NdeI restriction site sequence were designed to permit the initiation of protein translation at a methionine residue (encoded within the NdeI restriction site sequence, in the case of producing a non His-tagged recombinant protein) or to fuse in frame with the DNA sequence encoding the His-tag (for producing His tagged recombinant protein), followed by the coding sequence for the remainder of the native  H. pylori  DNA. The primer with the BamHI restriction site was produced to fuse the  H. pylori  specific sequence in-frame with the C-terminus of the glutathione-S-transferase gene in the pGEX vectors (Pharmacia LKB, Uppsala, Sweden). All reverse oligonucleotide primers designed to include an EcoRI restriction site at the 5′ terminus. Several reverse oligonucleotide primers were selected that would cause a truncation of the polypeptide to remove certain portions of the C-terminus, and in these cases the EcoRI restriction site at the 5′ end was followed by a translational termination codon. This combination of primers would enable the ORF of interest (or parts of the ORF of interest) to be cloned into pET28b (to produce a His-tagged recombinant protein), pET30a (to produce a non His tagged or native recombinant protein) or the pGEX-4T or pGEX-5× series (to produce a GST fusion protein). The pET28b vector provides sequence encoding an additional 20 amino-terminal amino acids (plus the methionine in the NdeI restriction site) including a stretch of six histidine residues which makes up the His-tag, whereas the pGEX vectors fuse the  H. pylori  protein to a 26,000 Da glutathione-S-transferase protein.  
     [0736] Genomic DNA prepared from  H. pylori  strain J99 (ATCC 55679) was used as the source of template DNA for the PCR amplification reactions (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). To amplify a DNA sequence containing a specific  H. pylori  ORF, genomic DNA (50 nanograms) was introduced into a reaction tube containing 200 nanograms of both the forward and reverse synthetic oligonucleotide primer specific for the ORF of interest, and 45 microliters of PCR SuperMix purchased (GibcoBRL Life Technologies, Gaithersburg, Md., USA) in a total of 50 microliters. The PCR SuperMix is supplied in 1.1× concentrations and contains 22 mM Tris-HCl (pH 8.4), 55 mM KCl, 1.65 mM MgCl 2 , 220 micromolar of each dATP, dCTP, dGTP and dTTP, 22 units recombinant Taq polymerase/ml and stabilizers. The following thermal cycling conditions were used to obtain amplified DNA products for each ORF using a Perkin Elmer Cetus/GeneAmp PCR System thermal cycler.  
               TABLE 16                          Oligonucleotide primers                     Gene and location   Sequence               Vac38- BamHI post signal sequence   CGGGATCCGAAGGTGATGGTGTTTAT       (SEQ ID NO: 360; SEQ ID NO: 5122)   ATAGG (SEQ ID NO: 10027)       Vac38- NdeI post signal sequence   CGCATATGGAAGGTGATGGTGTTTAT           ATAGGG (SEQ ID NO: 10028)       Vac38- EcoRI/stop codon (removes C-   GCGAATTCTCACTCTTTCCAATAGTTT       terminal third of protein)   GCTGCAGAGC (SEQ ID NO: 10029)       Vac38- EcoRI/stop codon (removes C-   CCGGAATTCTTAATCCCGTTTCAAATG       terminal 11 amino acids)   GTAATAAAGG (SEQ ID NO: 10030)       Vac38- EcoRI downstream of native stop   GCGAATTCCCTTTTATTTAAAAAGTGT       codon   AGTTATACC (SEQ ID NO: 10031)                                                  
 
     [0737] Upon completion of the thermal cycling reactions, each sample of amplified DNA was subjected to electrophoresis on 1.0% agarose gels. The DNA was visualized by exposure to ethidium bromide and long wave UV irradiation, and cut out in gel slices. DNA was purified using the Wizard PCR Preps kit (Promega Corp., Madison Wis., USA), and then subjected to digestion with BamHI and EcoRI (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). The digested PCR amplicon was then re-electrophoresed and purified as before.  
     [0738] Ligation of  H. Pylori  DNA Sequences into Cloning Vectors  
     [0739] The pOK12 vector (J. Vieira and J. Messing, Gene 100:189-194, 1991) was prepared for cloning by digestion with BamHI and EcoRI or NdeI and EcoRI in the case of Vac41 (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). The vectors were subjected to electrophoresis on 1.0% agarose gels and purified using the Wizard PCR Preps kit (Promega Corp., Madison Wis., USA). Following ligation of the purified, digested vector and the purified, digested amplified  H. pylori  ORF, the products of the ligation reaction were transformed into  E coli  JM109 competent cells according to standard methods (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). Individual bacterial colonies were screened for those containing the correct recombinant plasmids by incubating in LB broth overnight (plus 25 ug/ml kanamycin sulfate) followed by plasmid DNA preparation using the Magic Minipreps system (Promega Corp., Madison Wis., USA), and then analyzed by restriction digestion (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994).  
     [0740] Cloning of  H. Pylori  DNA Sequences into the pET28b, pET30a and pGEX4T-3 Prokaryotic Expression Vectors  
     [0741] Both the pET28b and pET30a expression vectors were prepared for cloning by digestion with NdeI and EcoRI, and the pGEX4T-3 vector was prepared for cloning by digestion with BamHI and EcoRI (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). The  H. pylori  DNA sequences were removed from pOK 12 plasmid backbones by digestion with NdeI and EcoRI or BamHI and EcoRI (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). The pET28b, pET30a, pGEX4T-3 and  H. pylori  DNA sequences were all electrophoresed on a 1% agarose gel and purified using the Wizard PCR Preps kit (Promega Corp., Madison Wis., USA). Following ligation of the purified, digested expression vector and the purified, digested  H. pylori  DNA sequences, the products of the ligation reaction were transformed into  E. coli  JM109 competent cells (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). Individual bacterial colonies were screened for those containing the correct recombinant plasmids by preparing plasmid DNA as described above followed by analysis by restriction digestion profiles and DNA sequencing (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). These recombinant plasmids were then used to transform specific  E coli  expression strains.  
     [0742] Transformation of Competent Bacteria with Recombinant Expression Plasmids  
     [0743] Competent bacterial strains BL21 (DE3), BL21 (DE3)pLysS, HMS174(DE3) and HMS174(DE3)pLysS were prepared and transformed with the recombinant pET28b expression plasmids carrying the cloned  H. pylori  sequences according to standard methods (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). These expression host strains contain a chromosomal copy of the gene for T7 RNA polymerase. These hosts are lysogens of bacteriophage DE3, a lambda derivative that carries the lacI gene, the lacUV5 promoter and the gene for T7 RNA polymerase. T7 RNA polymerase expression is induced by the addition of isopropyl-β-D-thiogalactoside (IPTG), and the T7 RNA polymerase then transcribes any target plasmid, such as pET28b, that carries a T7 promoter sequence and a gene of interest.  
     [0744] Competent bacterial strains JM109 and DH5α were prepared and transformed with the recombinant pGEX4T-3 expression plasmid carrying the cloned  H. pylori  sequences according to standard methods (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994).  
     [0745] Expression of Recombinant  H. Pylori  Sequences in  E. Coli    
     [0746] Transformants were collected from LB agar plates containing 25 ug/ml kanamycin sulfate (ensures maintenance of the pET28b-based recombinant plasmids) or 100 ug/ml ampicillin (ensures maintenance of the pGEX4T-3-based recombinant plasmids) and used to inoculate LB broth containing 25 ug/ml kanamycin sulfate or 100 ug/ml ampicillin and grown to an optical density at 600 nm of 0.5 to 1.0 OD units, at which point 1 mM IPTG was added to the culture for one to three hours to induce gene expression of the  H. pylori  recombinant DNA constructions. After induction of gene expression with IPTG, bacteria were pelleted by centrifugation and resuspended in SDS-PAGE solubilization buffer and subjected to SDS-PAGE (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). Proteins were visualized by staining with Coomassie Brilliant Blue or detected by western immunoblotting using the specific anti-His tag monoclonal antibody (Clontech, Palo Alto, Calif., USA) or the anti-GST tag antibody (Pharmacia LKB) using standard methods (Current Protocols in Molecular Biology, John Wiley and Sons, Inc., F. Ausubel et al., eds., 1994). The host strain that provided the highest level of recombinant protein production was then chosen for use in a large-scale induction in order to purify the recombinant protein. The strains used were HMS174(DE3) (pET28b-based constructs) and DH5α (pGEX4T-3-based constructs).  
     [0747] Removal of the C-terminal regions appeared in both systems to improve the level of expression, although this increase was far more prominent in the GST-fusion system. All recombinant proteins produced were of the predicted molecular weight based on the DNA sequence plus, if necessary, the size of the fusion tag. The truncated portion of the  H. pylori  protein contains some extremely hydrophobic stretches, and removal of these may be the reason for the increased expression.  
     [0748] Equivalents  
     [0749] Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments and methods described herein. Such equivalents are intended to be encompassed by the scope of the following claims.  
 
    
     
       
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                 SEQUENCE LISTING 
               
            
           
           
               
            
               
                 The patent application contains a lengthy “Sequence Listing” section. A copy of the “Sequence Listing” is available in electronic form from the USPTO 
               
               
                 web site (http://seqdata.uspto.gov/sequence.html?DocID=20040052799). An electronic copy of the “Sequence Listing” will also be available from the 
               
               
                 USPTO upon request and payment of the fee set forth in 37 CFR 1.19(b)(3).