Patent Publication Number: US-2012041211-A1

Title: Novel process for preparing carboxy-containing pyrazoleamido compounds 597

Description:
The present invention relates to processes for the preparation of a range of pharmaceutical compounds and intermediates used in the preparation. 
     WO2008/099145 discloses a range of chemical compounds, or pharmaceutically-acceptable salts thereof that possess human 11-β-hydroxysteroid dehydrogenase type 1 enzyme (11βHSD1) inhibitory activity and accordingly have value in the treatment of disease states including metabolic syndrome and are useful in methods of treatment of a warm-blooded animal, such as man. The invention also relates to processes for the manufacture of said compounds, to pharmaceutical compositions containing them and to their use in the manufacture of medicaments to inhibit 11βHSD1 in a warm-blooded animal, such as man. 
     In particular, the compounds of formula (1): 
     
       
         
         
             
             
         
       
     
     wherein:
 
Q, R 1 , R 2 , R 3 , X, Y and A are as defined in WO2008/099145,
 
or a pharmaceutically-acceptable salt thereof, are prepared by for example, by hydrolysis of an ester of formula (2):
 
     
       
         
         
             
             
         
       
     
     wherein R 22  is an alkyl or aryl group and R 1 , R 2 , R 3 , Q, A and X are as defined in relation to formula (I). 
     It has been found however that there are some problems associated with the preparation of intermediates of formula (2) when they are produced on a large scale. In particular, the synthesis of esters of formula (2) may be lengthy in that esters of starting materials such as compounds of formula (8) 
     
       
         
         
             
             
         
       
     
     where X, A and R 22  are as defined above, may be required to be produced specifically. 
     Furthermore, cyclisation reactions for example between compounds of formula (12) 
     
       
         
         
             
             
         
       
     
     where R 2 , R 3 , R 1 , X′ and Q are as defined in WO2008/099145 and (8) to yield compounds of formula (2) as recommended in WO2008/099145 may require large volumes, for example up to 200 relative volumes of solvent, such as methanol. Such large volume reactions are inefficient and wasteful of solvent. 
     According to the present invention there is provided a process for preparing a compound of formula (I) 
     
       
         
         
             
             
         
       
     
     wherein:
 
R 1  is C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 cycloalkyl, heterocyclyl, arylC 1-3 alkyl, heteroarylC 1-3 alkyl, C 3-7 cycloalkylC 1-3 alkyl, C 3-7 cycloalkylC 2-3 alkenyl or C 3-7 cycloalkylC 2-3 alkynyl, [each of which is optionally substituted by 1, 2 or 3 substituents independently selected from C 1-3 alkyl, hydroxy, halo, oxo, cyano, trifluoromethyl, C 1-3 alkoxy, C 1-3 alkylS(O) n — (wherein n is 0, 1, 2 or 3), R 5 CON(R 5′ )—, (R 5′ )(R 5″ )NC(O)—, R 5′ C(O)O—, R 5′ OC(O)—, (R 5′ )(R 5″ )NC(O)N(R 5′″ )—, R 5 SO 2 N(R 5″ )—, and (R 5′ )(R 5″ )NSO 2 — (wherein R 5  is C 1-3 alkyl optionally substituted by 1, 2 or 3 substituents selected from hydroxyl, halo or cyano; and
 
R 5′  and R 5″  are independently selected from hydrogen and C 1-3 alkyl optionally substituted by 1, 2 or 3 substituents independently selected from hydroxyl, halo, C 1-3 alkoxy, carboxy and cyano or R 5′  and R 5″  together with the nitrogen atom to which they are attached form a 4-7 membered saturated ring)];
 
R 2  is selected from heterocyclyl, C 3-7 cycloalkyl(CH 2 ) m —, and C 6-12 polycycloalkyl(CH 2 ) m — (wherein m is 0, 1 or 2 and the rings are optionally substituted by 1, 2 or 3 substituents independently selected from R 6 );
 
R 3  is selected from hydrogen, C 1-4 alkyl C 3-5 cycloalkyl and C 3-5 cycloalkylmethyl (each of which is optionally substituted by 1, 2 or 3 fluoro atoms);
 
R 2  and R 3  together with the nitrogen atom to which they are attached form a saturated mono, bicyclic or bridged ring system optionally containing 1 or 2 additional ring heteroatoms selected from nitrogen, oxygen and sulphur and which is optionally fused to a saturated, partially saturated or unsaturated monocyclic ring wherein the resulting ring system is optionally substituted by 1, 2, or 3 substituents independently selected from R 7 ;
 
R 6  and R 7  are independently selected from hydroxyl, halo, oxo, carboxy, cyano, trifluoromethyl, R 9 , R 9 O—, R 9 CO—, R 9 C(O)O—, R 9 CON(R 9′ )—, (R 9′ )(R 9″ )NC(O)—, (R 9′ )(R 9″ )N—, R 9 S(O) a — wherein a is 0 to 2, R 9′ OC(O)—, (R 9′ )(R 9″ )NSO 2 —, R 9 SO 2 N(R 9″ )—, (R 9′ )(R 9″ )NC(O)N(R 9′″ )—, phenyl and heteroaryl [wherein the phenyl and heteroaryl groups are optionally fused to a phenyl, heteroaryl or a saturated or partially-saturated 5- or 6-membered ring optionally containing 1, 2 or 3 heteroatoms independently selected from nitrogen, oxygen and sulphur and the resulting ring system is optionally substituted by 1, 2 or 3 substituents independently selected from C 1-4 alkyl, hydroxyl, cyano, trifluoromethyl, trifluoromethoxy, halo, C 1-4 alkoxy, C 1-4 alkoxyC 1-4 alkyl, amino, N—C 1-4 alkylamino, di-N,N—(C 1-4 alkyl)amino, N—C 1-4 alkylcarbamoyl, di-N,N—(C 1-4 alkyl)carbamoyl, C 1-4 alkylS(O) r —, C 1-4 alkylS(O) r C 1-4 alkyl (wherein r is 0, 1 or 2)];
 
R 9  is independently selected from C 1-3 alkyl optionally substituted by hydroxyl, halo, C 1-4 alkoxy, carboxy or cyano;
 
R 9′ , R 9″  and R 9′″  are independently selected from hydrogen and C 1-3 alkyl optionally substituted by 1, 2, or 3 substituents independently selected from hydroxyl, halo, C 1-4 alkoxy, carboxy and cyano);
 
A is a phenyl or heteroaryl ring (the phenyl or heteroaryl ring being optionally substituted on ring carbon atoms by 1, 2 or 3 R 10  groups and on an available ring nitrogen in a heteroaryl group by R 11 );
 
R 10  is independently selected from C 1-4 alkyl, hydroxyl, cyano, trifluoromethyl, trifluoromethoxy, halo, C 1-4 alkoxy, C 1-4 alkoxyC 1-4 alkyl, amino, N—C 1-4 alkylamino, di-N,N—(C 1-4 alkyl)amino, N—C 1-4 alkylcarbamoyl, di-N,N—(C 1-4 alkyl)carbamoyl, C 1-4 alkylS(O) s —, C 1-4 alkylS(O) s C 1-4 alkyl (wherein s is 0, 1 or 2)];
 
R 11  is independently C 1-3 alkyl optionally substituted by 1, 2 or 3 fluoro atoms;
 
X is a direct bond, C 3-4 cycloalkandiyl, C 3-4 cycloalkanylidene, —C(R 12 )(R 13 )—, —C(R 12 )(R 13 )C(R 14 )(R 15 )—, —CH 2 O— or —CH 2 S(O) t — (wherein t is 0, 1 or 2):
 
Y is a direct bond, C 3-4 cycloalkandiyl, C 3-4 cycloalkanylidene, —C(R 16 )(R 17 )— or —C(R 18 )(R 19 )C(R 20 )(R 21 )—;
 
wherein R 12 , R 13 , R 14 , R 15 , R 16 , R 17 , R 18 , R 19 , R 20  and R 21  are independently selected from hydrogen and methyl; which process comprises reacting a compound of formula (II)
 
     
       
         
         
             
             
         
       
     
     where X and A are as defined in relation to formula (I), with a compound of formula (III) 
     
       
         
         
             
             
         
       
     
     wherein R 1 , R 2  and R 3  are as defined above, and X′ represents either dialkylamino (such as dimethylamino) or lower alkoxy (such as methoxy or ethoxy);
 
and thereafter if necessary or desirable carrying out one or more or the following steps:
 
i) converting a compound of the formula (1) into another compound of the formula (1);
 
ii) removing any protecting groups;
 
iii) resolving enantiomers;
 
iv) forming a pharmaceutically-acceptable salt thereof;
 
v) purifying the product.
 
     The process has been found to be efficient in allowing compounds to be prepared directly from acids of formula (II), which may be available commercially. Furthermore, the reaction, which is generally carried out in an organic solvent such as methanol, appears to be require far less solvent than processes where the corresponding esters of formula (II) as shown as compound (8) above are used. 
     The process is suitably carried out using a suitable solvent such as methanol for example. Typically the reaction is carried out at ambient temperature, although elevated temperatures may be employed, for example the reflux temperature of the solvent. The reaction may be carried out in the presence of an acid such as hydrochloric acid as illustrated in the Examples below. 
     Examples of conversions of a compound of Formula (I) into another compound of Formula (I), well known to those skilled in the art, include functional group interconversions such as hydrolysis, hydrogenation, hydrogenolysis, oxidation or reduction, and/or further functionalisation by standard reactions such as amide or metal-catalysed coupling, or nucleophilic displacement reactions. 
     Purification procedures would also be well understood in the art. 
     Suitably, a purification step in which the product is dissolved in aqueous base such as aqueous sodium hydroxide and insoluble impurities removed by toluene extraction before acidifying the solution to recover the product is used. 
     Hydrazines of formula (II) are known in the chemical literature or may be prepared using standard conditions known to those skilled in the art. 
     Compounds of formula (III) may also be prepared by processes known in the art, for example as described in WO2008/099145. They are suitably prepared by reacting a compound of formula (IV) 
     
       
         
         
             
             
         
       
     
     where R 1 , R 2  and R 3  are as defined above, with an acetal of formula (V) 
     
       
         
         
             
             
         
       
     
     where X′ is as defined above. The reaction is suitably carried out in an organic solvent at temperatures in the range of from 85 to 95° C. Although WO2008/099145 suggests the use of 1,4-dioxane as a solvent, and treatment at high temperatures for example of 100° C. under nitrogen, followed by evaporation to dryness to obtain the required product, the applicants have found that more environmentally friendly solvents, in particular toluene or a mixture of toluene and n-heptane may be used in this stage and the product isolated by addition of a anti-solvent such as heptane, making the process much easier to perform. 
     Compounds of formula (V) are known compounds or may be prepared from known compounds by conventional methods. 
     Compounds of formula (IV) may be prepared in various ways for example as illustrated in WO2008/099145. 
     In a particular embodiment, the compound of formula (IV) is prepared by reacting a compound of formula (VI) 
     
       
         
         
             
             
         
       
     
     where R 1  is as defined above and R 23  is an alkyl group such as C 1-4 alkyl, in particular ethyl; with a compound of formula (VII) 
     
       
         
         
             
             
         
       
     
     The reaction is suitably effected in an organic solvent such as toluene or xylene, at an elevated temperatures for example in the range of from 100 to 110° C. The compound of formula (IV) is suitably isolated by addition of a suitable anti-solvent, such as n-heptane. The reaction of compounds of formula (VI) with compounds of formula (VII) is novel and forms a further aspect of the invention. It is advantageous over previous processes for the production of compounds of formula (IV) since it avoids operations such as evaporation to dryness and the use of halocarbon reagents such as dichloromethane. 
     Compounds of formula (VII) are suitably generated in situ in the solvent by addition of a base to a solution of a salt, for example an acid addition salt such as a hydrochloride salt of a compound of formula (VII). 
     The reactions described above may be performed under standard conditions known to the person skilled in the art. The intermediates described above are commercially available, are known in the art or may be prepared by known procedures and/or by the procedures shown above. 
     It will be appreciated that certain of the various substituents in the compounds of the present invention may be introduced by standard aromatic substitution reactions or is generated by conventional functional group modifications either prior to or immediately following the processes mentioned above, and as such are included in the process aspect of the invention. Such reactions and modifications include, for example, introduction of a substituent by means of an aromatic substitution reaction, reduction of substituents, alkylation of substituents and oxidation of substituents. The reagents and reaction conditions for such procedures are well known in the chemical art. Particular examples of aromatic substitution reactions include the introduction of a nitro group using concentrated nitric acid, the introduction of an acyl group using, for example, an acyl halide and Lewis acid (such as aluminium trichloride) under Friedel Crafts conditions; the introduction of an alkyl group using an alkyl halide and Lewis acid (such as aluminium trichloride) under Friedel Crafts conditions; and the introduction of a halogeno group. Particular examples of modifications include the reduction of a nitro group to an amino group by for example, catalytic hydrogenation with a nickel catalyst or treatment with iron in the presence of hydrochloric acid with heating; oxidation of alkylthio to alkylsulphinyl or alkylsulphonyl. 
     It will also be appreciated that in some of the reactions mentioned herein it may be necessary/desirable to protect any sensitive groups in the compounds. The instances where protection is necessary or desirable and suitable methods for protection are known to those skilled in the art. Conventional protecting groups may be used in accordance with standard practice (for illustration see T. W. Green, Protective Groups in Organic Synthesis, John Wiley and Sons, 1991). Thus, if reactants include groups such as amino, carboxy or hydroxy it may be desirable to protect the group in some of the reactions mentioned herein. 
     A suitable protecting group for an amino or alkylamino group is, for example, an acyl group, for example an alkanoyl group such as acetyl, an alkoxycarbonyl group, for example a methoxycarbonyl, ethoxycarbonyl or t-butoxycarbonyl group, an arylmethoxycarbonyl group, for example benzyloxycarbonyl, or an aroyl group, for example benzoyl. The deprotection conditions for the above protecting groups necessarily vary with the choice of protecting group. Thus, for example, an acyl group such as an alkanoyl or alkoxycarbonyl group or an aroyl group may be removed for example, by hydrolysis with a suitable base such as an alkali metal hydroxide, for example lithium or sodium hydroxide. Alternatively an acyl group such as a t-butoxycarbonyl group may be removed, for example, by treatment with a suitable acid as hydrochloric, sulphuric or phosphoric acid or trifluoroacetic acid and an arylmethoxycarbonyl group such as a benzyloxycarbonyl group may be removed, for example, by hydrogenation over a catalyst such as palladium-on-carbon, or by treatment with a Lewis acid for example boron tris(trifluoroacetate). A suitable alternative protecting group for a primary amino group is, for example, a phthaloyl group which may be removed by treatment with an alkylamine, for example hydroxylamine, or with hydrazine. 
     A suitable protecting group for a hydroxy group is, for example, an acyl group, for example an alkanoyl group such as acetyl, an aroyl group, for example benzoyl, or an arylmethyl group, for example benzyl. The deprotection conditions for the above protecting groups will necessarily vary with the choice of protecting group. Thus, for example, an acyl group such as an alkanoyl or an aroyl group may be removed, for example, by hydrolysis with a suitable base such as an alkali metal hydroxide, for example lithium or sodium hydroxide. Alternatively an arylmethyl group such as a benzyl group may be removed, for example, by hydrogenation over a catalyst such as palladium-on-carbon. 
     A suitable protecting group for a carboxy group is, for example, an esterifying group, for example a methyl or an ethyl group which may be removed, for example, by hydrolysis with a base such as sodium hydroxide, or for example a t-butyl group which may be removed, for example, by treatment with an acid, for example an organic acid such as trifluoroacetic acid, or for example a benzyl group which may be removed, for example, by hydrogenation over a catalyst such as palladium-on-carbon. 
     The protecting groups may be removed at any convenient stage in the synthesis using conventional techniques well known in the chemical art. 
     In this specification the term “alkyl” includes both straight and branched chain alkyl groups but references to individual alkyl groups such as “propyl” are specific for the straight chain version only. For example, “C 1-4 alkyl” includes propyl, isopropyl and t-butyl. However, references to individual alkyl groups such as ‘propyl’ are specific for the straight chain version only and references to individual branched chain alkyl groups such as ‘isopropyl’ are specific for the branched chain version only. A similar convention applies to other radicals therefore “C 1-4 alkoxyC 1-4 alkyl” would include 1-(C 1-4 alkoxy)propyl, 2-(C 1-4 alkoxy)ethyl and 3-(C 1-4 alkoxy)butyl. The term “halo” refers to fluoro, chloro, bromo and iodo. 
     Where optional substituents are chosen from “one or more” groups it is to be understood that this definition includes all substituents being chosen from one of the specified groups or the substituents being chosen from two or more of the specified groups. 
     A 4-7 membered saturated ring (for example formed between R 5′  and R 5″  and the nitrogen atom to which they are attached) is a monocyclic ring containing the nitrogen atom as the only ring atom. 
     “Heteroaryl”, unless otherwise specified, is a totally unsaturated, monocyclic ring containing 5 or 6 atoms of which at least 1, 2 or 3 ring atoms are independently chosen from nitrogen, sulphur or oxygen, which may, unless otherwise specified, be carbon-linked. A ring nitrogen atom may be optionally oxidised to form the corresponding N-oxide. Examples and suitable values of the term “heteroaryl” are thienyl, furyl, thiazolyl, pyrazolyl, isoxazolyl, imidazolyl, pyrrolyl, thiadiazolyl, isothiazolyl, triazolyl, pyrimidyl, pyrazinyl, pyridazinyl and pyridyl. Particularly “heteroaryl” refers to thienyl, furyl, thiazolyl, pyridyl, imidazolyl or pyrazolyl. 
     “Heterocycyl” is a 4-7 saturated, monocyclic ring having 1-3 ring heteroatoms selected from nitrogen, oxygen and sulphur. The ring sulphur may be optionally oxidised to SO 2 . 
     A C 3-7 cycloalkyl ring is a saturated carbon ring containing from 3 to 7 ring atoms. 
     A C 3-4 cycloalkandiyl ring is a saturated carbon ring containing 3 or 4 ring atoms. It is a diradical with the radicals on different ring carbon atoms. 
     A C 3-4 cycloalkanylidene ring is a saturated carbon ring containing 3 or 4 ring atoms. It is a diradical with the radicals on the same ring carbon atom. 
     A polycycloalkyl ring is a ring system in which either at least 2 rings are fused together or in which 2 ring have one ring atom in common (spiro). 
     A “saturated mono, bicyclic or bridged ring system optionally containing 1 or 2 additional ring heteroatoms selected from nitrogen, oxygen and sulphur”, unless otherwise specified contains 4-14 ring atoms. Particularly a mono ring contains 4-7 ring atoms, a bicyclic ring 6-14 ring atoms and a bridged ring system 6-14 ring atoms. Examples of mono rings include piperidinyl, piperazinyl and morpholinyl. Examples of bicyclic rings include decalin and 2,3,3a,4,5,6,7,7a-octahydro-1H-indene. 
     Bridged ring systems are ring systems in which there are two or more bonds common to two or more constituent rings. Examples of bridged ring systems include 1,3,3-trimethyl-6-azabicyclo[3.2.1]octane, 2-aza-bicyclo[2.2.1]heptane and 7-azabicyclo(2,2,1)heptane, 1- and 2-adamantanyl. 
     A “saturated, partially saturated or unsaturated monocyclic ring” is, unless otherwise specified, a 4-7 membered ring. Examples include, cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl and phenyl. 
     Examples of a “saturated or partially-saturated 5- or 6-membered ring optionally containing 1, 2 or 3 heteroatoms independently selected from nitrogen, oxygen and sulphur” include piperidinyl, piperazinyl and morpholinyl. 
     Examples of “C 1-4 alkoxy” include methoxy, ethoxy and propoxy. Examples of “C 1-4 alkoxyC 1-4 alkyl” include methoxymethyl, ethoxymethyl, propoxymethyl, 2-methoxyethyl, 2-ethoxyethyl and 2-propoxyethyl. Examples of “C 1-4 alkylS(O) n  wherein n is 0 to 2” include methylthio, ethylthio, methylsulphinyl, ethylsulphinyl, mesyl and ethylsulphonyl. Examples of “C 1-4 alkylS(O) q C 1-4 alkyl” wherein q is 0 to 2″ include methylthio, ethylthio, methylsulphinyl, ethylsulphinyl, mesyl, ethylsulphonyl, methylthiomethyl, ethylthiomethyl, methylsulphinylmethyl, ethylsulphinylmethyl, mesylmethyl and ethylsulphonylmethyl. Examples of “C 1-4 alkanoyl” include propionyl and acetyl. Examples of “N—(C 1-4 alkyl)amino” include methylamino and ethylamino. Examples of “N,N—(C 1-4 alkyl) 2 -amino” include N,N-dimethylamino, N,N-diethylamino and N-ethyl-N-methylamino. Examples of “C 2-4 alkenyl” are vinyl, allyl and 1-propenyl. Examples of “C 2-4 alkynyl” are ethynyl, 1-propynyl and 2-propynyl. Examples of “N—(C 1-4 alkyl)carbamoyl” are methylaminocarbonyl and ethylaminocarbonyl. Examples of “N,N—(C 1-4 alkyl) 2 -carbamoyl” are dimethylaminocarbonyl and methylethylaminocarbonyl. Examples of “C 3-7 cycloalkylC 1-3 alkalkyl” include cyclopropymethyl, 2-cyclopropylethyl, cyclobutylmethyl, cyclopentylmethyl and cyclohexylmethyl. Examples of “C 3-7 cycloalkylC 2-3 alkalkenyl” include 2-cyclopropylethenyl, 2-cyclopentylethenyl and 2-cyclohexylethenyl. Examples of “C 3-7 cycloalkylC 2-3 alkalkynyl” include 2-cyclopropylethynyl, 2-cyclopentylethynyl and 2-cyclohexylethynyl. 
     Examples of “C 3-7 cycloalkyl(CH 2 ) m -” include cyclopropymethyl, 2-cyclopropylethyl, cyclobutylmethyl, cyclopentylmethyl and cyclohexylmethyl. Examples of C 6-12 polycycloalkyl(CH 2 ) m — include norbornyl bicyclo[2.2.2]octane(CH 2 ) m —, bicyclo[3.2.1]octane(CH 2 ) m — and 1- and 2-adamantanyl(CH 2 ) m —. 
     A suitable pharmaceutically-acceptable salt of a compound of the invention is, for example, an acid-addition salt of a compound of the invention which is sufficiently basic, for example, an acid-addition salt with, for example, an inorganic or organic acid, for example hydrochloric, hydrobromic, sulphuric, phosphoric, trifluoroacetic, citric or maleic acid. In addition a suitable pharmaceutically-acceptable salt of a compound of the invention which is sufficiently acidic is an alkali metal salt, for example a sodium or potassium salt, an alkaline earth metal salt, for example a calcium or magnesium salt, an ammonium salt or a salt with an organic base which affords a physiologically-acceptable cation, for example a salt with methylamine, dimethylamine, trimethylamine, piperidine, morpholine or tris-(2-hydroxyethyl)amine. 
     Some compounds of the formula (1) may have chiral centres and/or geometric isomeric centres (E- and Z-isomers), and it is to be understood that the invention encompasses the preparation of all such optical, diastereoisomers and geometric isomers that possess 11βHSD1 inhibitory activity. 
     It is also to be understood that certain compounds of the formula (1) can exist in solvated as well as unsolvated forms such as, for example, hydrated forms. It is to be understood that the invention encompasses the production all such solvated forms, which possess 11βHSD1 inhibitory activity. 
     Particular examples of compounds of formula (I) are compounds of formula (IA): 
     
       
         
         
             
             
         
       
     
     wherein R 1 , R 2  and R 3  are as hereinabove defined and R 10  is selected from hydrogen, C 1-4 alkyl, trifluoromethyl, C 1-4 alkoxy and C 1-4 alkylS-. In another aspect R 10  is selected from hydrogen, methyl, trifluoromethyl, methoxy and methylthio. In another aspect R 10  is hydrogen. 
     Particular values of variable groups in compounds of formula (I) are as follows. Such values may be used where appropriate with any of the definitions, claims or embodiments defined hereinbefore or hereinafter, for compounds of formula (1). The definitions of R 1 , R 2  and R 3  and variables within those groups may be used for the compound of formula (IA): 
     Definition of R 1    
     a) In one aspect R 1  is C 3-6 cycloalkyl optionally substituted by 1, 2 or 3 substituents independently selected from C 1-3 alkyl, hydroxy, halo, oxo, cyano, fluoro, trifluoromethyl and C 1-3 alkoxy.
 
b) In another aspect R 1  is C 3-6 cycloalkyl.
 
c) In another aspect R 1  is C 3-6 cycloalkylC 1-2 alkyl optionally substituted by 1, 2 or 3 substituents independently selected from C 1-3 alkyl, hydroxy, halo, oxo, cyano, fluoro, trifluoromethyl and C 1-3 alkoxy.
 
d) In another aspect R 1  is C 3-4 cycloalkylC 1-2 alkyl.
 
e) In another aspect R 1  is C 1-4 alkyl optionally substituted by 1, 2 or 3 substituents independently selected from C 1-3 alkyl, hydroxy, halo, oxo, cyano, trifluoromethyl and C 1-3 alkoxy.
 
f) In another aspect R 1  is C 1-4 alkyl.
 
g) In another aspect R 1  is propyl optionally substituted by 1 or 2 substituents independently selected from C 1-3 alkyl, hydroxy, halo, oxo, cyano, trifluoromethyl and C 1-3 alkoxy.
 
h) In another aspect R 1  is tert-butyl
 
     Definition of R 2    
     a) In one aspect, R 2  is selected from C 3-7 cycloalkyl(CH 2 ) m —, and C 6-12 polycycloalkyl(CH 2 ) m — (wherein m is 0, 1 or 2 and the rings are optionally substituted by 1, 2 or 3 substituents independently selected from R 6 ) wherein m is 0, 1 or 2.
 
b) In another aspect, R 2  is selected from C 5-7 cycloalkyl(CH 2 ) m — and C 8-12 polycycloalkyl(CH 2 ) m — (wherein the rings are optionally substituted by 1, 2 or 3 substituents independently selected from R 6 ) and wherein m is 0, 1 or 2.
 
c) In another aspect, R 2  is selected from C 5-7 cycloalkyl(CH 2 ) m —, C 7-10 bicycloalkyl(CH 2 ) m — and C 10 tricycloalkyl(CH 2 ) m — (wherein the cycloalkyl, bicycloalkyl and tricycloalkyl rings are optionally substituted by 1, 2 or 3 substituents independently selected from R 6 ) and wherein m is 0, 1 or 2.
 
d) In yet another aspect, R 2  is selected from C 5-7 cycloalkyl(CH 2 ) m —, C 7-10 bicycloalkyl(CH 2 ) m — and adamantyl (wherein the cycloalkyl, bicycloalkyl and tricycloalkyl rings are optionally substituted by 1, 2 or 3 substituents independently selected from R 6 ) and wherein m is 0, 1 or 2.
 
e) In yet another aspect, R 2  is adamantly.
 
     Definition of m 
     a) In one aspect, m is 0 or 1.
 
b) In another aspect, m is 0.
 
     Definition of R 3    
     a) In one aspect, R 3  is C 1-4 alkyl.
 
b) In another aspect, R 3  is hydrogen, methyl or ethyl.
 
c) In another aspect, R 3  is hydrogen.
 
d) In another aspect, R 3  is methyl.
 
e) In another aspect, R 3  is ethyl.
 
f) In another aspect, R 3  is cyclopropyl.
 
     Definition of R 2  and R 3  Together 
     a) In another aspect, R 2  and R 3  together with the nitrogen atom to which they are attached form a saturated 5 or 6-membered mono, 6-12 membered bicyclic or 6-12 membered bridged ring system optionally containing 1 or 2 additional ring heteroatoms selected from nitrogen, oxygen and sulphur and which is optionally fused to a saturated, partially-saturated or aryl monocyclic ring wherein the resulting ring system is optionally substituted by 1, 2, or 3 substituents independently selected from R 7 . 
     Definition of R 6    
     a) In one aspect, R 6  is independently selected from hydroxyl, R 9 O—, R 9 CO— and R 9 C(O)O—
 
wherein R 9  is as hereinabove defined.
 
b) In another aspect, R 6  is independently selected from hydroxyl, R 9 O—, R 9 CO— and R 9 C(O)O—
 
wherein R 9  is C 1-3 alkyl optionally substituted by C 1-4 alkoxy or carboxy.
 
c) In another aspect, R 6  is independently selected from R 9 CON(R 9′ )—, R 9 SO 2 N(R 9″ )- and (R 9′ )(R 9″ )NC(O)N(R 9″ )—;
 
wherein R 9  is as hereinabove defined.
 
d) In another aspect, R 6  is independently selected from R 9 CON(R 9′ )—, R 9 SO 2 N(R 9″ )- and (R 9′ )(R 9″ )NC(O)N(R 9″ )—;
 
R 9  is C 1-3 alkyl optionally substituted by C 1-4 alkoxy or carboxy;
 
R 9′ , R 9″  and R 9″  are independently selected from hydrogen and C 1-3 alkyl optionally substituted by C 1-4 alkoxy or carboxy).
 
e) In another aspect, R 6  is independently selected from (R 9′ )(R 9″ )NC(O)— and (R 9′ )(R 9″ )N—;
 
wherein R 9′  and R 9″  are as hereinabove defined.
 
f) In another aspect, R 6  is independently selected from (R 9′ )(R 9″ )NC(O)— and (R 9′ )(R 9″ )N—;
 
wherein R 9′  and R 9″  are independently selected from hydrogen and C 1-3 alkyl optionally substituted by C 1-4 alkoxy or carboxy.
 
g) In one aspect R 6  is selected from methyl, trifluoromethyl, chloro, fluoro, bromo, methoxy, ethoxy, trifluormethoxy, methanesulfonyl, ethanesulfonyl, methylthio, ethylthio, amino, N-methylamino, N-ethylamino, N-propylamino, N,N-dimethylamino, N,N-methylethylamino or N,N-diethylamino.
 
h) In another aspect, R 6  is optionally substituted phenyl, pyridyl or pyrimidyl.
 
i) In another aspect, R 6  is optionally substituted pyrid-2-yl, pyrid-3-yl or pyrid-4-yl.
 
     Definition of R 7    
     a) In another aspect, R 7  is independently selected from hydroxyl, halo, oxo, cyano, trifluoromethyl, R 9  and R 9 O— (wherein R 9  is as hereinabove defined).
 
b) In another aspect, R 7  is independently selected from hydroxyl, halo, trifluoromethyl, R 9  and R 9 O— (wherein R 9  is as hereinabove defined).
 
     Definition of R 9    
     a) In one aspect, R 9  is independently selected from C 1-3 alkyl. 
     Definition of R 9′ , R 9″  and R 9′″   
     a) In one aspect, R 9′ , R 9″  and R 9′″  are independently selected from hydrogen and C 1-3 alkyl. 
     Definition of Y 
     a) In one aspect, Y is independently selected from direct bond, —CH 2 — and —CH 2 CH 2 —.
 
b) In one aspect, Y is independently selected from —CH 2 — and —CH 2 CH 2 —.
 
c) In another aspect Y is a direct bond.
 
     Definition of A 
     a) In one aspect A is phenyl optionally substituted by R 10 .
 
b) In another aspect A is heteroaryl optionally substituted by R 10  and R 11 .
 
c) In another aspect A is thienyl optionally substituted by R 10  and R 11 .
 
d) In another aspect A is pyridyl optionally substituted by R 10  and R 11 .
 
e) In another aspect A is phen-1,4-diyl
 
     Definition of R 10    
     a) In one aspect, R 10  is independently selected from C 1-4 alkyl, hydroxyl, cyano, trifluoromethyl, trifluoromethoxy, difluoromethoxy, halo, C 1-4 alkoxy and C 1-4 alkoxyC 1-4 alkyl.
 
b) In another aspect, R 10  is independently selected from methyl, ethyl, hydroxyl, cyano, trifluoromethyl, trifluoromethoxy, difluoromethoxy, halo, methoxy, ethoxy, methoxymethyl and ethoxymethyl.
 
c) In another aspect, R 10  is independently selected from methyl, ethyl, cyano, trifluoromethyl, trifluoromethoxy, difluoromethoxy, halo, methoxy, ethoxy.
 
     Definition of R 11    
     a) In one aspect, R 11 , is independently selected from C 1-3 alkyl, trifluoromethyl and difluoromethyl.
 
b) In one aspect, R 11 , is independently selected from methyl, ethyl, trifluoromethyl and difluoromethyl.
 
     Definition of X 
     a) In one aspect, X is independently selected from direct bond, —CH 2 —, —CHMe—, —CMe 2 -, —CH 2 CH 2 —, —CH 2 O— and —CH 2 S—.
 
b) In one aspect, X is independently selected from —CH 2 —, —CHMe—, —CMe 2 -, —CH 2 CH 2 —, —CH 2 O— and —CH 2 S—.
 
c) In another aspect X is independently selected from cyclopropanylidene, cyclobutanylidene, cyclopropane-1,2-diyl and cyclobutan-1,2-diyl.
 
d) In another aspect X is a direct bond.
 
     In one aspect, R 12 , R 13 , R 14 , R 15 , R 16 , R 17 , R 18 , R 19 , R 20  and R 21  are hydrogen. 
     In one aspect R 1  is optionally substituted by 0 substituents. 
     In one aspect R 1  is optionally substituted by 1 substituent. 
     In one aspect R 1  is optionally substituted by 2 substituents. 
     In one aspect R 1  is optionally substituted by 3 substituents. 
     In one aspect R 2  is optionally substituted by 0 substituents. 
     In one aspect R 2  is optionally substituted by 1 substituent. 
     In one aspect R 2  is optionally substituted by 2 substituents. 
     In one aspect R 2  is optionally substituted by 3 substituents. 
     In one aspect R 3  is optionally substituted by 0 substituents. 
     In one aspect R 3  is optionally substituted by 1 substituent. 
     In one aspect R 3  is optionally substituted by 2 substituents. 
     In one aspect R 3  is optionally substituted by 3 substituents. 
     In one aspect the group formed by R 2  and R 3  together is optionally substituted by 0 substituents. 
     In one aspect the group formed by R 2  and R 3  together is optionally substituted by 1 substituent. 
     In one aspect the group formed by R 2  and R 3  together is optionally substituted by 2 substituents. 
     In one aspect the group formed by R 2  and R 3  together is optionally substituted by 3 substituents. 
     In one aspect A is optionally substituted by 0 substituents. 
     In one aspect A is optionally substituted by 1 substituent. 
     In one aspect A is optionally substituted by 2 substituents. 
     In one aspect A is optionally substituted by 3 substituents. 
     In one aspect the phenyl and heteroaryl groups in R 6  and R 7  are independently optionally substituted by 0 substituents. 
     In one aspect the phenyl and heteroaryl groups in R 6  and R 7  are independently optionally substituted by 1 substituent. 
     In one aspect the phenyl and heteroaryl groups in R 6  and R 7  are independently are optionally substituted by 2 substituents. 
     In one aspect the phenyl and heteroaryl groups in R 6  and R 7  are independently are optionally substituted by 3 substituents. 
     In another aspect the invention relates to a process for preparing 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid or a pharmaceutically-acceptable salt thereof, which process comprises the step of reacting a compound of the formula (IIB): 
     
       
         
         
             
             
         
       
     
     or salt thereof; 
     with a compound of formula (IIIB): 
     
       
         
         
             
             
         
       
     
     and thereafter if necessary or desirable carrying out one or more of the following steps: 
     i) forming a pharmaceutically-acceptable salt thereof; and 
     ii) purifying the product. 
     In a particular aspect, the aryl hydrazine is a hydrochloride salt. 
     A particular example of a compound of formula (I) is 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid. In one aspect this is prepared in a pure polymorphic form. In particular, this compound is in a crystalline form (referred to herein as ‘Form 4’), which has an X-ray powder diffraction pattern with a peak at about 2-theta=16.2°, when measured using CuKa radiation. Suitably the compound is in a crystalline form which has an X-ray powder diffraction pattern with at least two specific peaks at about 2-theta=16.2° and 20.6°, for example with specific peaks at about 2-theta=16.2, 20.6 and 17.7°, more particularly with specific peaks at about 2-theta=16.2, 20.6, 17.7, is 10.8 and 15.5° and yet more particularly with specific peaks at about 2-theta=16.2, 20.6, 17.7, 10.8, 15.5, 20.9, 26.1, 11.6, 26.7 and 18.1°, wherein any of said values may be plus or minus 0.5° 2-theta. 
     For instance, the compound is in a crystalline form which has an X-ray powder diffraction pattern, using CuKa radiation, substantially the same as the X-ray powder diffraction pattern shown in  FIG. 1 . 
     
       
         
           
               
             
               
                 TABLE C 
               
             
            
               
                   
               
               
                 Ten most Prominent X-Ray Powder Diffraction 
               
               
                 peaks Form 4 of the Agent 
               
            
           
           
               
               
               
            
               
                   
                 Angle 2-Theta (2θ) 
                 Relative Intensity 
               
               
                   
                   
               
               
                   
                 16.2 
                 vs 
               
               
                   
                 20.6 
                 s 
               
               
                   
                 17.7 
                 s 
               
               
                   
                 10.8 
                 s 
               
               
                   
                 15.5 
                 m 
               
               
                   
                 20.9 
                 m 
               
               
                   
                 26.1 
                 m 
               
               
                   
                 11.6 
                 m 
               
               
                   
                 26.7 
                 m 
               
               
                   
                 18.1 
                 m 
               
               
                   
                   
               
            
           
         
       
     
     DSC analysis of Form 4 shows a peak at 262.0° C. followed by a subsequent melt with an onset of 312.0° C. The DSC thermogram of form 4 is depicted in  FIG. 2 . Such forms are obtainable using the process exemplified hereinafter. 
     Another more pure sample of form 4 gave the XPRD pattern shown in  FIG. 3 . The position of d-spacing are shown in table D. 
     
       
         
           
               
             
               
                 TABLE D 
               
               
                   
               
               
                 d-Spacing for form 4 
               
               
                 d-spacing [Å] 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 8.2 
               
               
                 7.7 
               
               
                 5.8 
               
               
                 5.6 
               
               
                 5.1 
               
               
                 4.76 
               
               
                 4.40 
               
               
                 4.32 
               
               
                 3.50 
               
               
                 3.44 
               
               
                   
               
            
           
         
       
     
     Another aspect of the invention relates to the preparation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 4 by recrysallisation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 1 from THF, water and acetonitrile. 
     Yet another aspect of the invention relates to the preparation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 4 by recrysallisation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 1 from THF and acetonitrile. 
     Yet another aspect of the invention relates to the preparation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 4 by recrysallisation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 1 from DMF and acetonitrile. 
     Yet another aspect of the invention relates to the preparation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 4 by recrysallisation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 1 from acetic acid and acetonitrile. 
     Yet another aspect of the invention relates to the preparation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 4 by recrysallisation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 1 from 2-methylTHF and acetonitrile. 
     Another aspect of the invention relates to the preparation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 4 by recrysallisation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as a hydrate from THF, water and acetonitrile. 
     Yet another aspect of the invention relates to the preparation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 4 by recrystallisation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as a hydrate from THF and acetonitrile. 
     Yet another aspect of the invention relates to the preparation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 4 by recrysallisation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as a hydrate from DMF and acetonitrile. 
     Yet another aspect of the invention relates to the preparation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 4 by recrysallisation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as a hydrate from acetic acid and acetonitrile. 
     Yet another aspect of the invention relates to the preparation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid as form 4 by recrysallisation of 4-[4-(2-adamantyl-carbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid a hydrate from 2-methylTHF and acetonitrile. 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 14.3 and 5.5 Å. 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 14.3, 5.5, 13.1 and 7.2 Å 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 14.3, 5.5, 13.1, 7.2 and 5.0 Å. 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 5.5 and 14.4 Å. 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 5.5, 14.4, 16.7 and 5.1 Å. 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 5.5, 14.4, 16.7, 5.1, 13.1 and 12.8 Å 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 8.1 and 13.2 Å, 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 8.1, 13.2, 17.9 and 5.9 Å 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 8.1, 13.2, 17.9, 5.9, 6.4 and 5.0 Å. 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 14.2 and 10.1 Å. 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 14.2, 10.1, 7.1 and 6.4 Å 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 14.2, 10.1, 7.1, 6.4, 5.6 and 4.50 Å 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 4.71 and 6.3 Å 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 4.71, 6.3, 7.6 and 4.12 Å 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 12.7 and 5.7 Å 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 12.7, 5.7, 16.3 and 5.5 Å. 
     Yet another aspect of the invention relates to a crystalline form of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid having an X-ray diffraction pattern with peaks at the following d-spacing values: 12.7, 5.7, 16.3, 5.5 and 4.23 Å. 
     The invention is illustrated by the following examples. 
    
    
     ABBREVIATIONS USED IN EXAMPLES 
     DCM=Di Chloro Methane 
     DMF=Di-Methyl Formamid 
     MIBK=Methyl Iso-Buthyl Keton 
     MTBE=Methyl Tert-Buthyl Ether 
     TGA=Thermo Gravimetric Analysis 
     THF=Tetra Hydro Furane 
     XRPD=X-Ray Powder Diffraction 
     STEP-1 
     Example 1 
     Synthesis of (2)-N-(2-adamantyl)-2-(dimethylaminomethylidene)-4,4-dimethyl-3-oxo-pentanamide 
     
       
         
         
             
             
         
       
     
     To a suspension of 2-adamantanamine hydrochloride (25.0 g, 0.13 mol) in water (75.0 ml, 3.0 rel. vol) was added toluene (100.0 ml, 4.0 rel. vol). A 10.0% w/w aqueous sodium hydroxide solution (1.25 mol. eq) was fed into the above solution and stirred for 10 to 15 minutes. The organic layer was separated and the aqueous layer re-extracted with toluene (75.0 ml, 3.0 rel. vol) and combined with the separated organic layer. The combined organic layer was washed with 5.0% w/w sodium chloride solution (75 ml, 3.0 rel. vol.) and separated. Ethyl pivaloylacetate (26.01 g, 0.15 mol) was added to the organic layer was the reaction mass heated to reflux at 110 to 112° C. The solvent (4 to 5 rel. vol.) was collected azeotropically over 4 to 5 hours. The reaction mass was cooled to 40 to 45° C. and n-heptane (200.0 ml, 8.0 rel. vol) added at 35 to 40° C. followed by DMF-DMA (26.45 g, 0.20 mol) and triethylamine (13.48 g, 0.13 mol) at 30 to 35° C. The reaction mass temperature was raised to 90 to 93° C. and maintained for 2 to 3 hours. The methanol generated as a by-product was collected azeotropically during the reaction. The reaction was cooled to 20 to 25° C. and stirred for 1.0 hr at that temperature. The precipitated product was filtered, bed washed with n-heptane (100.0 ml, 4.0 rel. vol) and the product dried under vacuum (50-100 mbar) at 35-40° C. for 3 to 4 hours to give (2)-N-(2-adamantyl)-2-(dimethylaminomethylidene)-4,4-dimethyl-3-oxo-pentanamide (Yield, 86%). The product was packed under nitrogen atmosphere and stored below 10° C. as it was found to be unstable at room temperature. 
     Example-2 
     To a suspension of 2-adamantanamine hydrochloride (25.0 g, 0.13 mol) in water (75.0 ml, 3.0 rel. vol) was added toluene (100.0 ml, 4.0 rel. vol). A 10.0% w/w aqueous sodium hydroxide solution (1.25 mol. eq) was fed into the above solution and stirred for 10 to 15 minutes. The organic layer was separated and the aqueous layer re-extracted with toluene (75.0 ml, 3.0 rel. vol) and combined with the separated organic layer. The combined organic layer was washed with 5.0% w/w sodium chloride solution (75 ml, 3.0 rel. vol.) and separated. Ethyl pivaloylacetate (26.01 g, 0.15 mol) was added to the organic layer was the reaction mass heated to reflux at 110 to 112° C. The solvent (4 to 5 rel. vol.) was collected azeotropically over 4 to 5 hours. The reaction mass was cooled to 40 to 45° C. and n-heptane (200.0 ml, 8.0 rel. vol) added at 35 to 40° C. followed by DMF-DMA (26.45 g, 0.20 mol) at the same temperature. The reaction mass temperature was raised to 85 to 90° C. and maintained for 4 to 5 hours. The methanol generated as a by-product was collected azeotropically during the reaction. The reaction was cooled to 20 to 25° C. and stirred for 1.0 hr at that temperature. The precipitated product was filtered, bed washed with n-heptane (100.0 ml, 4.0 rel. vol) and the product dried under vacuum (50-100 mbar) at 35-40° C. for 3 to 4 hours to give (2)-N-(2-adamantyl)-2-(dimethylaminomethylidene)-4,4-dimethyl-3-oxo-pentanamide (Yield, 72%). The product was packed under nitrogen atmosphere and stored below 10° C. as it was found to be unstable at room temperature. 
     Chromatographic Conditions:— 
     Sunfire C18, 150×4.6 mm, 5μ, mobile phase used is di-sodium hydrogen phosphate buffer using methanol as organic solvent, 1.0 mL/min flow rate, injection volume is 204, run time is 20 minutes using refractive index detector. 
     Retention Times: 
     N-(2-adamantyl)-4,4-dimethyl-3-oxo-pentanamide RT: 11.0 min
 
(2)-N-(2-adamantyl)-2-(dimethylaminomethylidene)-4,4-dimethyl-3-oxo-pentanamide RRT: 1.18 min.
 
     1H NMR (400.13 MHz, DMSO-d6) δ 1.13 (9H, s), 1.47 (2H, d), 1.69-1.83 (10H, m), 2.03 (2H, d), 2.92 (6H, s), 3.90 (1H, d), 7.24 (1H, s), 7.94 (1H, d) 
     m/z (ESI+) (M+H)+=333 
     If necessary the N-(2-adamantyl) — 4,4-dimethyl-3-oxopentanamide intermediate may be isolated: 
     Chromatographic Conditions: — 
     HP-5MS column, Helium as carrier gas, 1.0 mL/min flow rate, solvent delay up to 1.5 min, oven temperature=initial 50° C., hold for 2 min, and then ramping @20° C./min up to 280° C. and injection volume is 1.04. 
     Retention Times: 
     2-Adamantanamine Hydrochloride RT 8.1 min 
     N-(2-adamantyl)-4,4-dimethyl-3-oxo-pentanamide RRT: 1.617 min. 
     1H NMR (400.13 MHz, DMSO-d6) δ 1.08-1.09 (9H, m), 1.50 (2H, d), 1.66-1.89 (10H, m), 1.95-2.00 (2H, m), 3.53 (1.4H, s), 3.80-3.94 (1H, m), 5.30 (0.3H, s), 7.77-7.87 (1H, m), 14.43 (0.3H, s) (2:1 mixture of keto and enol forms) m/z (ESI+) (M+H)+=278 
     STEP-2 
     Synthesis of 4-[4-(2-Adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid (Form-1) 
     
       
         
         
             
             
         
       
     
     4-Hydrazinobenzoic acid.HCl (14.11 g, 0.075 mol), and (2)-N-(2-adamantyl)-2-(dimethylaminomethylidene)-4,4-dimethyl-3-oxo-pentanamide (25.0 g, 0.075 mol) were put into a jacketed reactor followed by isopropyl alcohol (315 ml, 12.6 rel. vol.) and water (35 ml, 1.4 rel. vol.). The reaction mass was stirred at 20 to 25° C. for about 45 to 60 minutes. The contents were heated to reflux at 78 to 80° C. and maintained at that temperature for 90 minutes. The reaction mass was cooled to 50 to 55° C. and then water (150 ml, 6 rel. vol.) added at the same temperature. The contents were further cooled to ambient temperature (20 to 25° C.) and stirred for 1.0 hour at the same temperature. The precipitated product was filtered and then washed with a mixture of 1:1 ratio of isopropyl alcohol:water (250 ml, 10.0 rel. vol.) to yield 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid form 1. The product was dried under vacuum at 50 to 55° C. for 4 to 5 hours and used in the next step without further purification (Yield: 80%). 
     1H NMR (400.13 MHz, DMSO-d6) δ 1.19 (9H, s), 1.49 (2H, d), 1.70-1.96 (10H, m), 2.09 (2H, d), 3.98-4.01 (1H, m), 7.49-7.53 (2H, m), 7.61 (1H, s), 8.06-8.09 (2H, m), 8.20 (1H, d), 13.30 (1H, s) 
     m/z (ESI+) (M+H)+=422 
     m.p. 308.8° C. (onset) 
     Chromatographic Conditions:— 
     Zorbax SB-Aq, 150×4.6 mm, 5μ, mobile phase used is formic acid buffer using acetonitrile as organic solvent, 1.0 mL/min flow rate, injection volume is 20 μL, run time is 18 minutes using UV detector wavelength 220,320 nm. 
     Retention Times: 
     [((2)-N-(2-Adamantyl)-2-(dimethylaminomethylidene)-4,4-dimethyl-3-oxo-pentanamide RT 14.2 min 
     4-[4-(2-Adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid RRT 0.77 min (10.0 min) 
     Intermediate RRT 0.79 (11.2 min) 
     STEP (3) 
     Polymorphs Conversion (Form-1 to Form-4) 
     4-[4-(2-Adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid 
     
       
         
         
             
             
         
       
     
     4-[4-(2-Adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid form 1 (20.0 g, 0.047 mol) followed by tetrahydrofuran (9.0 rel. vol) and water (0.5 rel vol) were added to a suitable jacketed reactor. The contents were stirred for 15 minutes, filtered through filter paper and washed with tetrahydrofuran (1.0 rel. vol). The combined filtrate was transferred to reactor and temperature of mass increased to 58 to 62° C. Acetonitrile (20.0 rel. vol) was added whilst maintaining the temperature at 55 to 65° C. The temperature of reaction mass was increased to 68±2° C. and maintained there for 22 hours. The contents were cooled to 20 to 25° C. and stirred for 2 hours. The product was filtered and the bed washed with acetonitrile (5.0 rel. vol). The wet cake was dried under vacuum (50-100 mbar) at 45 to 50° C. for 4 hours to yield polymorph form 4 (80%). 
     Alternatively: 
     Tetrahydrofuran (9.0 rel. vol) and water (0.5 rel vol) were added to 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid form 1 (20.0 g, 0.047 mol) and the mixture stirred for 15 minutes and then filtered through filter paper. The residue was washed with tetrahydrofuran (1.0 rel. vol) and the combined filtrate transferred to a reactor and the reaction temperature raised to 58 to 62° C. Acetonitrile (20.0 rel. vol) was added whilst maintaining the reaction at 55 to 65° C. The reaction temperature was raised to 68±2° C., maintained there for 22 hours, then cooled to 20 to 25° C. and stirred for 2 hours. The product was filtered and the bed washed with acetonitrile (5.0 rel. vol). The wet cake was dried under vacuum (50-100 mbar) at 45 to 50° C. for 4 hours to give polymorph 4 (yield 80%) as confirmed by XRPD. 
     Alternatively: 
     Tetrahydrofuran (10.0 rel. vol) was added to 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid form 1 (5.0 g, 0.012 mol) and the temperature raised to 58 to 62° C. Acetonitrile (20.0 rel. vol) was added whilst maintaining the reaction at 55 to 65° C. The temperature of the reaction was maintained at 68±2° C. for 20 hours. The contents were cooled to 20 to 25° C. and stirred for 2 hours. The product was filtered and the wet cake washed with acetonitrile (5.0 vol) and then dried in a vacuum oven (50-100 mbar) at 45 to 50° C. for 4 hours to give polymorph 4 (yield 90%) as confirmed by XRPD and Solid state NMR. 
     Alternatively: 
     N,N-Dimethylformamide (5.0 rel. vol) and acetonitrile (5.0 vol) were added to 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid form 1 (5.0 g, 0.012 mol) and the reaction temperature raised to 60 to 65° C. Acetonitrile (15.0 rel. vol) was added whilst maintaining the temperature at 55 to 65° C. The temperature of the reaction was raised to 75 to 78° C. and maintained there for 20 hours. The contents were cooled to 20 to 25° C. and stirred for 2 hours. The product was filtered and bed washed with acetonitrile (5.0 rel. vol) and then dried in a vacuum oven (50-100 mbar) at 45 to 50° C. for 4 hours to give polymorph 4 (yield 88%) as confirmed by XRPD. 
     Alternatively: 
     Acetic acid (10.0 rel. vol) was added to 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid form 1 (5.0 g, 0.012 mol) and the temperature raised to 75 to 78° C. Acetonitrile (20.0 rel. vol) was added whilst maintaining the temperature at 70 to 78° C. The mixture was stirred at 75 to 78° C. and maintained there for 22 hours. The contents were cooled to 20 to 25° C. and stirred for 2 hours. The product was filtered and the bed washed with acetonitrile (5.0 rel. vol) and then dried in a vacuum oven (50-100 mbar) at 45 to 50° C. for 4 hours to polymorph 4 (yield 66%) as confirmed by XRPD. 
     Alternatively: 
     2-Methyl-THF (10.0 rel. vol) was added to 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid form 1 (5.0 g, 0.012 mol) and the temperature raised to 70 to 75° C. Acetonitrile (20.0 rel. vol) was added whilst maintaining the temperature at 70 to 75° C. and then allowed to stir at 75 to 78° C. for 23 hours. The contents were cooled to 20 to 25° C. and stirred for 2 hours. The product was filtered and the bed washed with acetonitrile (5.0 rel. vol) and then dried in a vacuum oven (50-100 mbar) at 45 to 50° C. for 4 hours to give polymorph 4 (yield 93%) as confirmed by XRPD. 
     Synthesis of 4-[4-(2-Adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid (Hydrate) 
     
       
         
         
             
             
         
       
     
     4-Hydrazinobenzoic acid.HCl (2.86 g, 0.010 mol), water (10 mL) and methanol (60 mL) were added to a suitable flask and then a solution of (2)-N-(2-adamantyl)-2-(dimethylaminomethylidene)-4,4-dimethyl-3-oxo-pentanamide (5.10 g, 0.010 mol) in methanol (30 mL) was added. The contents were stirred at 20-25° C. for 1 hour and then heated to reflux at 65-66° C. for 90 minutes. The reaction mass was cooled to 40 to 45° C. and then 25% aqueous NaOH solution (2.0 eq.) was added slowly and continued to stir at the same temperature for 60 minutes. The contents were further cooled to ambient temperature (20 to 25° C.) and 10% aqueous HCl (2.7 eq) was added slowly and continued to stir for 60 minutes at the same temperature. Charged water (50 mL), precipitated product was filtered and then washed the bed with water (25 mL) followed by a mixture of 1:1 ratio of methanol:water (50 mL) to yield 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid (hydrate). The product was dried under vacuum at 50 to 55° C. for 4 to 5 hrs to give hydrated form as confirmed by XRPD (Yield: 86%). 
     1H NMR (400.13 MHz, DMSO-d6) δ 1.19 (9H, s), 1.49 (2H, d), 1.70-1.96 (10H, m), 2.09 (2H, d), 3.98-4.01 (1H, m), 7.49-7.53 (2H, m), 7.61 (1H, s), 8.06-8.09 (2H, m), 8.20 (1H, d) 
     m/z (ESI+) (M+H)+=422 
     m.p. 309.10° C. (onset) 
     Polymorphs Conversion (Hydrate to Form-4) 
     4-[4-(2-Adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid (form 4) 
     Tetrahydrofuran (10.0 rel. vol) was added to 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl] benzoic acid hydrate from the previous step (4.0 g, 0.009 mol) and then the reaction temperature raised to 58-62° C. Acetonitrile (20.0 rel. vol) was added whilst maintaining the reaction at 55-65° C. The reaction temperature was raised to 68±2° C., maintained there for 22 hours, then cooled to 20 to 25° C. and stirred for 2 hours. The product was filtered and the bed washed with acetonitrile (5.0 rel. vol). The wet cake was dried under vacuum (50-100 mbar) at 45 to 50° C. for 4 hours to give polymorph 4 (yield 80%) as confirmed by XRPD. 
     Form 5 
     4. 20 g of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid was charged crude into a 500 ml reactor followed by tetrahydrofuran (180.00 mL) and water (10.00 ml). The reaction mass was stirred for 20 minutes and became a clear solution. The solution was filtered and a line wash off. tetrahydrofuran (20.00 mL) was added. The combined filtrate was charged into the reactor and the temperature of the reaction mass raised to 60° C. 
     4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid, Form 4 seed was added to the solution and did not dissolve. The reaction mass was stirred for 1 hour until growth appeared to have stopped on the Lasentec probe. The reaction mass was cooled to 15° C. over 5 hours then stirred for one hour. The product (solid) was filtered under vacuum. The product was sucked dry for 5 minutes then dried under vacuum (50° C.) overnight. This solid was analyzed by XRPD (cobolt source) giving substantially the following d-values. See  FIG. 4  for diffractogram. 
     Peaks for Form 5 
       
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 d-spacing [Å] 
                 Rel. Int. [%] 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 14.3 
                 vs 
               
               
                   
                 13.1 
                 m 
               
               
                   
                 8.2 
                 vw 
               
               
                   
                 7.7 
                 vw 
               
               
                   
                 7.2 
                 m 
               
               
                   
                 6.5 
                 vw 
               
               
                   
                 5.9 
                 w 
               
               
                   
                 5.8 
                 w 
               
               
                   
                 5.5 
                 m 
               
               
                   
                 5.0 
                 m 
               
               
                   
                 4.82 
                 w 
               
               
                   
                 4.40 
                 w 
               
               
                   
                 4.25 
                 w 
               
               
                   
                 4.20 
                 vw 
               
               
                   
                   
               
            
           
         
       
     
     Form 6 
     10 mL of tetrahydrofuran:water (10:0.5) was added to a polyblock vial containing ˜400 mg of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid of Form 4. The reaction mass was stirred at 22° C. for 2 days. All the solid had dissolved after two days. The solution was collected in a beaker. Within 5 minutes, solid crashed out of the solution. This solid was analyzed by XRPD (cobolt source) giving substantially the following d-values. See  FIG. 5  for diffractogram. 
     Peaks Form 6 
       
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 d-spacing [Å] 
                 Rel. Int. [%] 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 16.7 
                 m 
               
               
                   
                 14.4 
                 s 
               
               
                   
                 13.1 
                 s 
               
               
                   
                 12.8 
                 m 
               
               
                   
                 10.9 
                 vw 
               
               
                   
                 7.2 
                 w 
               
               
                   
                 6.9 
                 vw 
               
               
                   
                 6.5 
                 m 
               
               
                   
                 6.0 
                 m 
               
               
                   
                 5.8 
                 m 
               
               
                   
                 5.5 
                 vs 
               
               
                   
                 5.13 
                 s 
               
               
                   
                 5.08 
                 s 
               
               
                   
                 5.00 
                 m 
               
               
                   
                 4.82 
                 m 
               
               
                   
                 4.69 
                 w 
               
               
                   
                 4.39 
                 m 
               
               
                   
                 4.26 
                 m 
               
               
                   
                 3.95 
                 m 
               
               
                   
                 3.73 
                 m 
               
               
                   
                 3.42 
                 w 
               
               
                   
                 3.21 
                 w 
               
               
                   
                 3.05 
                 vw 
               
               
                   
                   
               
            
           
         
       
     
     Hemihydrate 
     Polymorphs Conversion to Hemihydrate 
     To the 4 necked RB flask equipped with stirrer and thermometer was charged 4-Hydrazino benzoic acid. HCl (2.88 g). Isopropyl Alcohol (50 mL) was charged into the reaction mass and the reaction mass was stirred for 5 minutes at 23-25° C. 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid, enamine (5.1 g [Limiting Reagent]; 1.00 equivalents; 14.99 mmoles; 5.10 g; [Actual]) were dissolved in Isopropyl Alcohol (50 mL). Then the dissolved enamine solution was added to 4-hydrazino benzoic acid solution in IPA at 23-25° C. The reaction mass was stirred for 1 hour at 23-25° C. for 1 hour. The reaction mass was heated to reflux at 80° C. The reaction mass was maintained for 1 hour at a reflux temperature of 80° C. Once the reaction was over the reaction mass was cooled to 25° C. and stirred at 25° C. for 1 hour. 50 mL of purified water was added and stirred at 25° C. for 1 hour. Subsequently it was stirred for another 30 minutes. Thereafter it was filtered through a buckerner funnel. Isopropyl alcohol and water (50:50) (50 g) was given for washing. Then it was suck dried for 15 minutes. Thereafter it was dried under vacuum for 12 hours at 50° C. Moisture content was confirmed by KF. (2% w/w). The solid was analysed by XRPD (cobolt source) giving substantially the following d-values. See  FIG. 6  for diffractogram. 
     Peaks for Hemihydrate 
       
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 d-spacing [Å] 
                 Rel. Int. [%] 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 17.9 
                 w 
               
               
                   
                 13.2 
                 s 
               
               
                   
                 9.3 
                 w 
               
               
                   
                 8.1 
                 vs 
               
               
                   
                 6.6 
                 w 
               
               
                   
                 6.4 
                 s 
               
               
                   
                 5.9 
                 s 
               
               
                   
                 5.6 
                 w 
               
               
                   
                 5.4 
                 m 
               
               
                   
                 5.2 
                 m 
               
               
                   
                 4.98 
                 s 
               
               
                   
                 4.74 
                 m 
               
               
                   
                 4.67 
                 m 
               
               
                   
                 4.58 
                 w 
               
               
                   
                 4.42 
                 m 
               
               
                   
                 4.17 
                 w 
               
               
                   
                 4.06 
                 m 
               
               
                   
                 3.72 
                 m 
               
               
                   
                 3.62 
                 w 
               
               
                   
                   
               
            
           
         
       
     
     DMF Solvate 
     5.6 g of Form 1 of 4-[4-(2-adamantylcarbamoyl)-5-tert-butyl-pyrazol-1-yl]benzoic acid in 40 mL of dimethylformamide was heated it for two days at 50° C., then cooled at 25° C., filtered and dried in vacuum for 4 hours. TGA and DSC was performed on the sample, which indicated it to be a dimethylformamide-solvate. The sample was analysed by XRPD (Cobolt source) giving substantially the following d-values. For diffractogram see  FIG. 7 . 
     Peaks for DMF Solvate 
       
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 d-spacing [Å] 
                 Rel. Int. [%] 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 14.2 
                 vs 
               
               
                   
                 10.1 
                 s 
               
               
                   
                 7.1 
                 m 
               
               
                   
                 6.4 
                 s 
               
               
                   
                 5.6 
                 s 
               
               
                   
                 5.4 
                 w 
               
               
                   
                 5.3 
                 vw 
               
               
                   
                 5.1 
                 w 
               
               
                   
                 4.99 
                 m 
               
               
                   
                 4.77 
                 m 
               
               
                   
                 4.50 
                 s 
               
               
                   
                 4.46 
                 w 
               
               
                   
                 4.17 
                 vw 
               
               
                   
                 3.96 
                 m 
               
               
                   
                 3.69 
                 w 
               
               
                   
                 3.22 
                 w 
               
               
                   
                 3.19 
                 m 
               
               
                   
                   
               
            
           
         
       
     
     EtOH-Solvate 
     Single crystal X-ray diffraction analysis, using Mo-source with wavelength 0.71073 Å and a graphite monochromator, showed EtOH-solvate to crystallize in the orthorhombic space group P2 1 2 1 2 1  with 4 molecules in the unit cell. The unit cell dimensions were found to be: a=7.0600(2) 
     b=16.1280(4)
 
c=22.0580(4)
 
α=90°
 
β13=90°
 
γ=90°
 
     V=2511.61 Å 3    
     Z=4 
     The calculated density is D c =1.24 g/cm 3 . 
     MeOH Solvate 
     20 mg of Form 4 was added to a small vessel. 0.711 ml of Form 4 saturated MeOH solution was added to the vessel making a suspension. The suspension was stirred for 7 days at 25° C. Thereafter the suspension was stirred at 21° C. for 1½ months. The wet suspension was analysed by XRPD (Cu-source) giving substantially the following d-values. The material is instable and on exposure to the lab atmosphere (21° C., 30% relative humidity) this form rapidly transforms to Form 3. For diffractogram see  FIG. 8 . 
     Peaks for Me OH-Solvate 
       
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 d-spacing (Å) 
                 Relative intensity 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 9.3 
                 w 
               
               
                   
                 7.6 
                 m 
               
               
                   
                 6.3 
                 m 
               
               
                   
                 5.9 
                 m 
               
               
                   
                 5.7 
                 m 
               
               
                   
                 5.5 
                 w 
               
               
                   
                 5.0 
                 m 
               
               
                   
                 4.87 
                 m 
               
               
                   
                 4.71 
                 vs 
               
               
                   
                 4.27 
                 w 
               
               
                   
                 4.1 
                 m 
               
               
                   
                 3.88 
                 m 
               
               
                   
                 3.25 
                 m 
               
               
                   
                 3.21 
                 m 
               
               
                   
                 3.16 
                 w 
               
               
                   
                   
               
            
           
         
       
     
     MTBE Solvate 
     20 mg of Form 4 was added to a small vessel. 0.711 ml MTBE was added to the vessel making a suspension. The suspension was stirred for 7 days at 25° C. The wet suspension was analysed by XRPD (Cu-source) giving substantially the following d-values. The material is instable and on exposure to the lab atmosphere (21° C., 30% relative humidity) this form transforms to Hydrate. 
     See  FIG. 9  for diffractogram. 
     Peaks for MTBE Solvate 
       
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 d- spacing (Å) 
                 Relative intensity 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 16.3 
                 m 
               
               
                   
                 12.7 
                 vs 
               
               
                   
                 5.7 
                 s 
               
               
                   
                 5.5 
                 m 
               
               
                   
                 5.4 
                 m 
               
               
                   
                 4.42 
                 m 
               
               
                   
                 4.23 
                 m 
               
               
                   
                 3.05 
                 m 
               
               
                   
                   
               
            
           
         
       
     
     X-Ray Powder Diffraction 
     The X-ray diffraction (referred to herein as XRPD) analysis was performed according to standard methods, which can be found in e.g. Kitaigorodsky, A. I. (1973), Molecular Crystals and Molecules, Academic Press, New York; Bunn, C. W. (1948), Chemical Crystallography, Clarendon Press, London; or Klug, H. P. &amp; Alexander, L. E. (1974), X-ray Diffraction Procedures, John Wiley &amp; Sons, New York. X-ray powder diffraction data were measured without any internal reference. 
     The X-ray powder diffraction patterns was determined by mounting a thin layer of the sample on a zero background holder, single silicon crystal or on a stainless steel holder with 2 mm depth. The samples were spun (to improve counting statistics) and automatic variable divergence slits were used. 
     Form 5, Form 6, Hemi-hydrate and DMF-solvate were analysed using a PanAlytical X′Pert PRO theta-2 theta diffractometer with an Xcelerator detector. The X-rays were generated by a cobalt tube operated at 40 kV and 30 mA with a wavelength of 1.78901 angstroms. 
     MeOH-solvate and MTBE-solvate were analysed using a PanAlytical X′Pert PRO theta-2 theta diffractometer with an Xcelerator detector. The X-rays were generated by a copper tube operated at 40 kV and 30 mA with a wavelength of 1.5406 angstroms. 
     The X-ray powder diffraction (XRPD) patterns in this were obtained in Bragg-Brentano geometry. Persons skilled in the art of X-ray powder diffraction will realise that the relative intensity of peaks can be affected by, for example, grains above 30 microns in size and non-unitary aspect ratios that may affect analysis of samples. The skilled person will also realise that the position of reflections can be affected by the precise height at which the sample sits in the diffractometer and the zero calibration of the diffractometer. The surface planarity of the sample may also have a small effect. 
     It is known that an X-ray powder diffraction pattern may be obtained which has one or more measurement errors depending on measurement conditions (such as equipment or machine used). No internal standard was used in any of the XRPD analyses and therefore the diffraction pattern data presented are not to be taken as absolute values. In particular, it is generally known that intensities in an X-ray powder diffraction pattern may fluctuate depending on experimental conditions and sample preparation (e.g. preferred orientation). (Jenkins, R &amp; Snyder, R. L. ‘Introduction to X-Ray Powder Diffractometry’ John Wiley &amp; Sons 1996; Bunn, C. W. (1948), Chemical Crystallography, Clarendon Press, London; Klug, H. P. &amp; Alexander, L. E. (1974), X-Ray Diffraction Procedures). 
     The following definitions of relative intensity have been used. 
     
       
         
           
               
               
             
               
                   
               
               
                 % Relative Intensity 
                 Definition 
               
               
                   
               
             
            
               
                  81-100 
                 vs (very strong) 
               
               
                 31-80 
                 s (strong) 
               
               
                 11-30 
                 m (medium) 
               
               
                  6-10 
                 w (weak) 
               
               
                 3-5 
                 vw (very weak)