Patent Publication Number: US-2023140027-A1

Title: Cell-culturing instrument-machining device

Description:
TECHNICAL FIELD 
     The present invention relates to a processing apparatus for a cell culture tool. 
     BACKGROUND ART 
     When a cultured cell mass is processed into a desired shape, the cultured cell mass is subjected to a treatment such as cutting the cell mass into a desired shape, or a cell culture tool is processed in advance such that the cultured cell mass has a desired shape (Patent Document 1). 
     CITATION LIST 
     Patent Literature 
     
         
         Patent Literature 1: JP H03(1991)-007576 A 
       
    
     SUMMARY OF INVENTION 
     Technical Problem 
     According to the production method of Patent Literature 1, the cell culture tool is processed to obtain the cultured cell mass in a desired shape by patterning the surface of the cell culture tool through photolithography. However, photolithography requires various manufacturing facilities including a photomask forming apparatus and an exposure apparatus. Accordingly, there has been a demand for a processing apparatus having a simple structure and capable of controlling the shape of cells in a cell culture tool. 
     In light of the foregoing, it is an object of the present invention to provide a processing apparatus for cell culture tools, capable of controlling a region to which cells can adhere in a cell culture tool including a cell culture base layer and a photothermal conversion layer. 
     Solution to Problem 
     In order to achieve the above object, the present invention provides a processing apparatus for a cell culture tool (hereinafter also referred to simply as “processing apparatus”), including: a laser irradiation unit capable of applying a laser to a photothermal conversion layer of a cell culture tool including a cell culture base layer and the photothermal conversion layer; and a control unit for controlling the laser irradiation unit, wherein the control unit includes a setting section and an irradiation control section, the setting section sets an irradiation region to be irradiated with the laser in the cell culture tool, and the irradiation control section controls the laser irradiation unit based on the irradiation region such that the laser irradiation unit apples the laser to a corresponding region of the photothermal conversion layer. 
     Advantageous Effects of Invention 
     The processing apparatus according to the present invention can control a region to which cells can adhere in a cell culture tool including a cell culture base layer and a photothermal conversion layer. 
    
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         FIG.  1    shows schematic views showing an example of the structure of a culture tool according to a first embodiment.  FIG.  1 A  is a schematic perspective view of the culture tool of the first embodiment.  FIG.  1 B  is a schematic cross-sectional view of the culture tool of the first embodiment as viewed along arrows I-I in  FIG.  1 A .  FIG.  1 C  is a plan view of the culture tool of the first embodiment. 
         FIG.  2    shows schematic views illustrating an example of a production method and a processing method of the culture tool of the first embodiment. 
         FIG.  3    is a perspective view showing the structure of a processing apparatus according to a second embodiment. 
         FIG.  4    shows schematic views showing the processing apparatus of the second embodiment.  FIG.  4 A  is a block diagram showing an example of the structure of a control unit of the processing apparatus of the second embodiment.  FIG.  4 B  is a block diagram showing the structure of a CPU in the control unit. 
         FIG.  5    is a flowchart illustrating the steps of a processing method executed by the control unit of the processing apparatus of the second embodiment. 
         FIG.  6    shows an example of the structure of a control unit of a processing apparatus according to a third embodiment. 
         FIG.  7    is a flowchart illustrating an example of processing executed by the control unit in the third embodiment. 
         FIG.  8    shows schematic views illustrating a method of setting an irradiation region in the third embodiment. 
         FIG.  9    shows schematic views showing an example of control of a laser irradiation unit by an irradiation control section in the third embodiment. 
         FIG.  10    shows schematic views showing the structure of a processing apparatus according to a fourth embodiment. 
         FIG.  11    is a flowchart illustrating an example of processing executed by the processing apparatus of the fourth embodiment. 
         FIG.  12    is a perspective view showing an example of a processing apparatus according to a fifth embodiment. 
         FIG.  13    is a perspective view showing an example of a first region in the processing apparatus of the fifth embodiment. 
         FIG.  14    is a cross-sectional view of the first region as viewed along arrows I-I in  FIG.  12   . 
         FIG.  15 A  is an exploded perspective view showing an example of a culture vessel placement portion in the processing apparatus of the first embodiment.  FIG.  15 B  is a cross-sectional view as viewed along arrows III-III in  FIG.  15 A . 
         FIG.  16    is a perspective view showing an example of the first region and a circulator in a state where an outer wall of the first region is removed in the processing apparatus of the fifth embodiment. 
         FIG.  17    is a cross-sectional view showing an upper part of the first region and the circulator as viewed along arrows II-II in  FIG.  12   . 
         FIG.  18 A  is a perspective view showing an example of the structure of a second region in the processing apparatus of the fifth embodiment.  FIG.  18 B  is a perspective view showing another example of the structure of the second region. 
         FIG.  19    is a block diagram showing an example of the structure of a control section of the processing apparatus of the fifth embodiment. 
         FIG.  20    is a perspective view showing another example of the processing apparatus of the fifth embodiment. 
         FIG.  21    shows schematic views illustrating a method of setting an irradiation region in the third embodiment. 
         FIG.  22    shows schematic views illustrating another method of setting an irradiation region in the third embodiment. 
     
    
    
     DESCRIPTION OF EMBODIMENTS 
     In the present invention, “cells” means, for example, isolated cells, or a cell mass (spheroid), tissue, or an organ composed of cells. The cells may be, for example, cultured cells or cells isolated from a living body. The cell mass, tissue, or organ may be, for example, a cell mass, cell sheet, tissue, or organ produced from the cells, or may be a cell mass, tissue, or organ isolated from a living body. The cells are preferably cells that adhere in an extracellular matrix (ECM)-dependent manner. 
     In the following, the processing apparatus according to the present invention and a cell culture tool to be processed by the processing apparatus will be described in details with reference to the drawings. It is to be noted, however, that the present invention is not limited by the following description. In  FIGS.  1  to  22    to be described below, identical parts are given the same reference numerals, and duplicate explanations thereof may be omitted. In the drawings, the structure of each part may be shown in a simplified form as appropriate for the sake of convenience in explanation, and each part may be shown schematically with a dimensional ratio and the like that are different from the actual dimensional ratio and the like. 
     First Embodiment 
     The present embodiment relates to an example of a cell culture tool to be processed by the processing apparatus of the present invention, an example of a method of producing the cell culture tool, and an example of a method of processing the cell culture tool using the processing apparatus of the present invention.  FIG.  1    shows schematic views showing the structure of a culture tool  100  of the first embodiment.  FIG.  1 A  is a schematic perspective view of the culture tool  100 .  FIG.  1 B  is a schematic cross-sectional view of the culture tool  100  as viewed along arrows I-I in  FIG.  1 A .  FIG.  1 C  is a plan view of the culture tool  100 . As shown in  FIG.  1   , the culture tool  100  includes a cell culture base layer  11 , a photothermal conversion layer  13 , and a vessel  12 , which is a cell culture tool. In the present embodiment, the cell culture base layer  11  includes a cell adhesion region  11   a  to which cells can adhere. The vessel  12  has a bottom surface  12   a  and a side wall  12   b . The cell culture base layer  11  is stacked on the bottom surface  12   a . The photothermal conversion layer  13  is arranged between the cell culture base layer  11  and the bottom surface  12   a . In other words, the photothermal conversion layer  13  and the cell culture base layer  11  are stacked on the bottom surface  12   a  in this order. As will be described below, in the present embodiment, a cell adhesion inhibitory region in which adhesion of cells is inhibited is formed by irradiating the culture tool  100  with light (laser) to change the adhesiveness of a cell culture base in the cell culture base layer  11 . The culture tool  100  is used for cell culture after the cell adhesion inhibitory region is formed. Accordingly, the culture tool  100  can also be referred to as a culture tool before cell culture or a culture tool not yet provided with a layer of cells. 
     The cell culture base layer  11  is a layer containing a cell culture base. The cell culture base means, for example, a substance that serves as a scaffold of cells during cell culture. The cell culture base may be, for example, an extracellular matrix (ECM) or a substance that has a function as a scaffold for cells. Examples of the extracellular matrix include: elastin; entactin; collagens such as type I collagen, type II collagen, type III collagen, type IV collagen, type V collagen, and type VII collagen; tenascin; fibrillin; fibronectin; laminin; vitronectin; proteoglycans each composed of a sulfated glycosaminoglycan such as chondroitin sulfate, heparan sulfate, keratan sulfate, or dermatan sulfate and a core protein; glucosaminoglycans such as chondroitin sulfate, heparan sulfate, keratan sulfate, dermatan sulfate, and hyaluronic acid; Synthemax® (vitronectin derivative), and Matrigel® (a mixture of laminin, type IV collagen, heparin sulfate proteoglycan, entactin/nidogen, etc.). Of these, laminin is preferable. Examples of the laminin include laminin  111 , laminin  121 , laminin  211 , laminin  213 , laminin  222 , laminin  311  (laminin  3 A 11 ), laminin  332  (laminin  3 A 32 ), laminin  321  (laminin  3 A 21 ), laminin  3 B 32 , laminin  411 , laminin  421 , laminin  423 , laminin  521 , laminin  522 , and laminin  523 . The three numbers in each laminin indicate, from the first to the last, the names of the constituent subunits of the α, β, and γ chains, respectively. As a specific example, laminin  111  is composed of α1, β1, and γ1 chains. The laminin  3 A 11  is composed of α3A, β1, and γ1 chains. Examples of the cell culture base may further include peptide fragments of the above-described proteins and fragments of the above-described sugar chains. Specifically, examples of the peptide fragments of the proteins include fragments of laminins. Examples of the fragment of laminins include fragments of the above-described laminins, and specific examples thereof include laminin  211 -E 8 , laminin  311 -E 8 , laminin  411 -E 8 , and laminin  511 -E 8 . The laminin  211 -E 8  is composed of fragments of the α2, β1, and γ1 chains of laminin. The laminin  311 -E 8  is composed of fragments of the α3, β1, and γ1 chains of laminin. The laminin  411 -E 8  is composed of fragments of the α4, β1, and γ1 chains of laminin. The laminin  511 -E 8  is composed of, for example, fragments of the α5, β1, and γ1 chains of laminin. 
     The cell culture base can be denatured indirectly by applying light (laser) to the photothermal conversion layer  13 , as will be described below. Specifically, the applied light is converted into heat, and the structure of the cell culture base is changed by the thus-generated thermal energy, thereby causing the indirect denaturation. In other words, the cell culture base is denatured by heat generated through the above-described light irradiation. 
     Although the culture tool  100  of the present embodiment includes one cell culture base layer  11 , the culture tool  100  may include two or more cell culture base layers  11 . 
     The cell culture base layer  11  may contain other components in addition to the cell culture base. Examples of the other components include buffers, salts, growth factors (cell growth factors), cytokines, and hormones. 
     In the culture tool  100  of the present embodiment, the cell culture base layer  11  is arranged (formed) only on the upper surface of the photothermal conversion layer  13 . However, the present invention is not limited thereto. The cell culture base layer  11  need only be arranged in a region that allows contact with cells, for example, and may be arranged on an inner peripheral surface of the side wall  12   b  instead of or in addition to the upper surface of the photothermal conversion layer  13  in the culture tool  100 . The cell culture base layer  11  may be formed on part or the whole of the region that allows contact with the cells. In the former case, the cell culture base layer  11  is preferably formed on the photothermal conversion layer  13  of the vessel  12  at the time of culturing cells. 
     The cell adhesion region  11   a  is a region to which the cells can adhere in the cell culture base layer  11 . The cell culture base is adherable to the cells in, for example, an undenatured state. Accordingly, the cell adhesion region  11   a  can also be referred to as a region containing the cell culture base in an undenatured state, i.e., a region containing the undenatured cell culture base. Part or the whole of the cell culture base contained in the cell adhesion region  11   a  is in an undenatured state. When part of the cell culture base is in an undenatured state, for example, at least 50%, at least 60%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the cell culture base in the cell adhesion region  11   a  is in an undenatured state. The proportion of the cell culture base in an undenatured state or a denatured state in the cell culture base can be determined by, for example, collecting the cell adhesion region  11   a , performing native polyacrylamide gel electrophoresis (native PAGE) on the thus-collected material, and determining the proportion based on a change in the band position. The cell adhesion region  11   a  can also be referred to as, for example, a region that has not been irradiated with light in the production method to be described below. In the present embodiment, the cell culture base is denatured indirectly through light irradiation, whereby the adhesion capacity thereof with the cells is deteriorated. Thus, the cell adhesion region  11   a  contains the cell culture base in an undenatured state. It is to be noted, however, that the present invention is not limited thereto, and the cell culture base may be such that the adhesion with cells is inhibited when it is in an undenatured state and the adhesion capacity thereof with cells is improved when it is denatured either directly or indirectly through light irradiation. In this case, the cell adhesion region  11   a  contains the cell culture base in a denatured state. The cell culture base becomes adherable to cells when it is denatured indirectly through light irradiation. 
     The vessel  12  can be used to culture cells. In the vessel  12 , a space surrounded by the bottom surface  12   a  and the side wall  12   b  is a region where cells can be cultured (cell culture region), and may also be referred to as a well, for example. The vessel  12  may be a cell culture vessel, and specific examples thereof includes substrates, dishes, plates, and flasks (cell culture flasks). The size, volume, and material of the vessel  12 , the presence or absence of an adhesion treatment, and the like can be determined as appropriate according to the type and amount of cells to be cultured in the culture tool  100 . The bottom surface  12   a  may be flat or substantially flat, and also may be a rough surface. Although the vessel  12  has the side wall  12   b  in the present embodiment, the side wall  12   b  may or may not be present. When the vessel  12  does not have the side wall  12   b , the vessel  12  can also be referred to as, for example, a substrate or a support. 
     The material of the vessel  12  is not limited to particular materials, and may be, for example, a material that transmits a laser applied by a laser irradiation unit to be described below. Specific examples of such a material include plastic and glass that transmit a laser. Examples of the plastic include polystyrene polymers, acrylic polymers (such as polymethyl methacrylate (PMMA)), polyvinylpyridine polymers (such as poly(4-vinylpyridine) and 4-vinylpyridine-styrene copolymers), silicone polymers (such as polydimethylsiloxane), polyolefin polymers (such as polyethylene, polypropylene, and polymethylpentene), polyester polymers (such as polyethylene terephthalate (PET) and polyethylene naphthalate (PEN)), polycarbonate polymers, and epoxy polymers. 
     Although the vessel  12  has one cell culture region, the vessel  12  may have a plurality of cell culture regions. In the latter case, it can also be said that the vessel  12  has a plurality of wells, for example. Also, in the latter case, the cell culture base layer  11  and the photothermal conversion layer  13  may be formed in any one of the cell culture regions, in some of the cell culture regions, or in all the cell culture regions. In other words, in the vessel  12 , the cell culture base layer  11  and the photothermal conversion layer  13  may be formed in any one or more of the wells or in all of the wells. 
     In the present embodiment, the vessel  12  may include a lid. The lid can cover the top of the vessel  12  in a detachable manner, for example. The lid is placed so as to face the bottom surface  12   a , for example. The lid may be, for example, a lid of the cell culture vessel. 
     The photothermal conversion layer  13  is a layer capable of converting light into heat. The photothermal conversion layer  13  contains, for example, molecules capable of converting light into heat (photothermal conversion molecules). Preferably, the photothermal conversion molecules are composed of, for example, a polymer (macromolecules) containing a dye structure (chromophore) that absorbs light L having a wavelength used for irradiation in a processing method for the culture vessel  100  to be described below. Preferably, the photothermal conversion molecules can be easily coated onto the vessel  12 . Examples of the dye structure that absorbs light L include derivatives of organic compounds, such as azobenzene, diarylethene, spiropyran, spirooxazine, fulgide, leuco dyes, indigo, carotinoid (such as carotene), flavonoid (such as anthocyanin), and quinoid (such as anthraquinone). Examples of the skeleton constituting the polymer include acrylic polymers, polystyrene polymers, polyolefin polymers, polyvinyl acetate and polyvinyl chloride, polyolefin polymers, polycarbonate polymers, and epoxy polymers. As a specific example, the photothermal conversion molecule may be, for example, poly[methylmethacrylate-co-(disperse yellow-7-methacrylate)]((C 5 H 8 O 2 ) m (C 23 H 20 N 4 O 2 ) n ) represented by the following formula (1). In the following formula (1), as the structure of azobenzene in the polymer, not only unsubstituted azobenzene but also any of various structures modified with a nitro group, an amino group, a methyl group, or the like may be employed. In the following formula (1), m and n each represent a mole percentage. The sum of m and n is, for example, 100 mol %. m and n may be the same or different from each other, for example. The photothermal conversion layer  13  may contain, for example, one type of photothermal conversion molecules or two or more types of photothermal conversion molecules. 
     
       
         
         
             
             
         
       
     
     Although one photothermal conversion layer  13  is provided in the culture tool  100  of the first embodiment, a plurality of photothermal conversion layers  13  may be provided. In this case, it is preferable to arrange the plurality of photothermal conversion layers  13  between the cell culture base layer  11  and the bottom surface  12   a . Although the photothermal conversion layer  13  is arranged in contact with the cell culture base layer  11  in the culture tool  100  of the present embodiment, it may be arranged so as not to be in contact with the cell culture base layer  11 . In this case, the photothermal conversion layer  13  and the cell culture base layer  11  may be thermally connected to each other. Specifically, a heat conductive layer for transferring heat generated in the photothermal conversion layer  13  to the cell culture base layer  11  is formed between the photothermal conversion layer  13  and the cell culture base layer  11 . The heat conductive layer contains molecules with high thermal conductivity, such as molecules of a metal, for example. 
     The photothermal conversion layer  13  may contain other components in addition to the above-described photothermal conversion molecules. The other components may be, for example, a polymer curing agent and unpolymerized monomers. 
     Although the photothermal conversion layer  13  is present only on the upper surface of the bottom surface  12   a  in the culture tool  100  of the present embodiment, the present invention is not limited thereto. The photothermal conversion layer  13  need only be arranged adjacent to the cell culture base layer  11 , for example, and may be formed inside the vessel  12 , for example. In this case, the photothermal conversion layer  13  is preferably formed on the upper surface of the bottom surface  12   a  of the vessel  12 . 
     Although the photothermal conversion layer  13  is present on the entire upper surface of the bottom surface  12   a  in the culture tool  100  of the present embodiment, the present invention is not limited thereto. The photothermal conversion layer  13  may be formed on a portion of the bottom surface  12   a , for example. 
     Although the photothermal conversion layer  13  is present only on the upper surface of the bottom surface  12   a  in the culture tool  100  of the present embodiment, the present invention is not limited thereto. The photothermal conversion layer  13  need only be arranged so as to be thermally connected to the cell culture base layer  11 , for example, and may be arranged on the inner peripheral surface of the side wall  12   b  instead of or in addition to the upper surface of the bottom surface  12   a  in the culture tool  100 . Also, the photothermal conversion layer  13  may be formed so as to be thermally connected to part or the whole of the cell culture base layer  11 . In the former case, the photothermal conversion layer  13  is preferably formed on the bottom surface  12   a  of the vessel  12  at the time of culturing cells. 
     Next, a method for producing the culture tool  100  (the production method according to the present embodiment) and a processing method for controlling a region to which cells can adhere in the culture tool  100  (the processing method according to the present embodiment) will be described with reference to  FIG.  2   .  FIG.  2    shows schematic views illustrating an example of the production method and the processing method of the culture tool  100 . In the production method of the culture tool  100  of the present embodiment, using a cell culture base in an undenatured state, a cell culture base layer  11  is formed on a photothermal conversion layer  13 . Then, in the processing method according to the present embodiment, the photothermal conversion layer  13  is irradiated with light, whereby the light is converted into heat by the photothermal conversion layer  13 . Thus, in the production method according to the present embodiment, by heat generated in the photothermal conversion layer  13 , the cell culture base in the cell culture base layer  11  adjacent to a region where the heat is generated is denatured, whereby a cell adhesion inhibitory region  11   b  is formed. 
     In the production method of the present embodiment, first, a vessel  12  is prepared, as shown in  FIG.  2 A  (preparation step). The vessel  12  may be purchased commercially or prepared in-house, as described above. 
     Next, in the production method of the present embodiment, a photothermal conversion layer  13  containing the above-described photothermal conversion molecules is formed on a bottom surface  12   a  of the vessel  12 , as shown in  FIG.  2 B  (conversion layer forming step). The photothermal conversion layer  13  can be formed by, for example, a known film formation method, and specific examples of the method include coating, printing (screening), vapor deposition, sputtering, casting, and spin coating. Specifically, the photothermal conversion layer  13  can be formed, for example, by introducing, through spin coating, casting, or the like, a raw material solution containing the dye structure-containing polymer described above or a raw material solution obtained by dissolving the dye structure-containing polymer in a solvent into the vessel  12 , more specifically such that the raw material solution is in contact with the bottom surface  12   a  of the vessel  12 , and then curing the raw material solution. Examples of the solvent include organic solvents such as 1,2-dichloroethane and methanol. 
     Next, in the production method of the present embodiment, a cell culture base layer  11  containing the cell culture base described above is formed on the photothermal conversion layer  13 , as shown in  FIG.  2 C  (base layer forming step). In this manner, the vessel  12  provided with the cell culture base layer  11  and the photothermal conversion layer  13  can be prepared by the production method of the present embodiment. In the production method of the present embodiment, the cell culture base used for forming the cell culture base layer  11  is in an undenatured state. The cell culture base is adherable to cells when it is in an undenatured state. Accordingly, as shown in  FIG.  2 C , the formed cell culture base layer  11  consists of a cell adhesion region  11   a . The cell culture base layer  11  can be formed by, for example, a known film formation method, and specific examples thereof include coating, printing (screening), vapor deposition, sputtering, casting, and spin coating. In the case where the cell culture base is a biopolymer such as a protein, the cell culture base layer  11  is preferably formed by coating, which can inhibit denaturation of the cell culture base. In this case, the cell culture base layer  11  may be formed by, for example, introducing a solvent containing an undenatured cell culture base into the vessel  12  and then allowing the vessel  12  to stand still. The solvent is, for example, an aqueous solvent and preferably water. The time period for which the vessel  12  is allowed to stand still is, for example, 30 minutes to one day. The temperature at which the vessel  12  is allowed to stand still is, for example, 4° C. to 40° C. In the case where the cell culture base is laminin  511 -E 8  and the coating concentration is 0.5 μg/cm 2 , the vessel  12  is allowed to stand still for at least one hour at a temperature of about 37° C. (35° C. to 39° C.), for example. In the base layer forming step, after the vessel  12  is allowed to stand still as described above, the solvent containing the undenatured cell culture base is removed. After the removal of the solvent, the inside of the vessel  12  may be washed with a solvent that does not contain the cell culture base. 
     Next, in the culture tool  100  of the present embodiment, a region to which cells can adhere is demarcated through light (laser) irradiation. The light irradiation is performed using a processing apparatus according to the present invention to be described below. In the processing method of the present embodiment, as shown in  FIG.  2 D , the vessel  12  (cell culture tool) provided with the cell culture base layer  11  and the photothermal conversion layer  13  is irradiated with light L to denature the cell culture base, whereby a cell adhesion inhibitory region  11   b  is formed (inhibitory region forming step). Specifically, in the inhibitory region forming step, the photothermal conversion layer  13  is irradiated with light L. More specifically, the photothermal conversion layer  13  is irradiated with light L with the light L being focused on the photothermal conversion layer  13 . As described above, the photothermal conversion layer  13  contains photothermal conversion molecules that convert light into heat. Accordingly, the photothermal conversion layer  13  irradiated with light L converts light energy of the light L into thermal energy. As a result, the temperature of a region of the photothermal conversion layer  13  irradiated with the light L increases, and this in turn increases the temperature of a region of the cell culture base layer  11  adjacent to the region irradiated with the light L, thereby changing the structure of the cell culture base in the cell culture base layer  11 . In the inhibitory region forming step, the cell culture base is denatured in this manner, whereby a cell adhesion inhibitory region  11   b  is formed. The light L is preferably controlled so as to be focused on the photothermal conversion layer  13 . In the inhibitory region forming step, it is preferable that the solvent is present on the cell culture base layer  11 . In the production method of the present embodiment, the cell culture base is adherable to the cells when it is in an undenatured state, for example. Accordingly, the light L is applied to a region of the photothermal conversion layer  13  corresponding (adjacent) to a region for forming the cell adhesion inhibitory region  11   b . More specifically, in  FIG.  4 D , the light L is applied to the corresponding region of the photothermal conversion layer  13 , present immediately below the region for forming the cell adhesion inhibitory region  11   b.    
     The wavelength of the light L can be set as appropriate according to the absorption wavelength of the photothermal conversion molecules contained in the photothermal conversion layer  13 . The light L may have a wavelength of, for example, ultraviolet light, visible light, or infrared light. As a specific example, in the case of the polymer represented by the formula (1), the wavelength of the light L is 390 to 420 nm, for example. The light is preferably a laser beam because it allows the cell adhesion inhibitory region  11   b  to be formed precisely. The spot diameter (beam width) of the light L can be set as appropriate according to the amount of energy of the light L, for example. When the light L has a relatively small amount of energy, the spot diameter is set relatively small. When the light L has a relatively large amount of energy, the spot diameter is set relatively large. The spot diameter of the light L is, for example, 10 to 200 m. The amount of energy (output power) of the light L is, for example, the amount of energy to denature the cell culture base of the cell culture base layer  11  present in a region corresponding to (adjacent to) a portion irradiated with the light L in the photothermal conversion layer  13 , and can be set as appropriate according to the type of the cell culture base and the type of the photothermal conversion molecules. The amount of energy of the light L is preferably the amount of energy to achieve a temperature at which cells in a cell layer stacked on the cell culture base layer  11  die. As a specific example, the amount of energy of the light L is the amount of energy to increase the temperature of the cell culture base in the cell culture base layer  11  at a portion irradiated with the light L to 50° C. or more, 60° C. or more, 70° C. or more, 80° C. or more, or 90° C. or more, and preferably to 100° C. or more, 110° C. or more, or 120° C. or more. The upper limit of the temperature is, for example, 200° C. In the inhibitory region forming step, the light L may be applied, for example, to increase the temperature of the photothermal conversion layer  13  so as to cause the cell culture base to have a temperature given above as examples. The scanning speed of the light L can be set as appropriate according to the spot diameter and the amount of energy of the light L, for example. When the amount of light energy per unit area of the spot diameter is relatively low, the scanning speed of the light L is set relatively low. When the amount of energy light per unit area of the spot diameter is relatively high, the scanning speed of the light L is set relatively high. As a specific example, the scanning speed of the light L is, for example, 100 mm/sec or less. The amount of energy of the light L is about 0.5 W (0.3 to 0.7 W) when the light L is a visible-light laser (405 nm), the spot diameter is 45 m, and the scanning speed of the light L is 80 mm/sec. 
     The cell adhesion inhibitory region  11   b  is a region where the adhesion of cells is inhibited. As described above, the cell culture base is adherable to cells when, for example, it is in an undenatured state. Thus, the cell adhesion inhibitory region  11   b  can also be referred to as, for example, a region containing the cell culture base in a denatured state, i.e., a region containing a thermally denatured product of the cell culture base. Part or the whole of the cell culture base contained in the cell adhesion inhibitory region  11   b  is in a denatured state. When part of the cell culture base is in a denatured state, for example, at least 50%, at least 60%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the cell culture base in the cell adhesion inhibitory region  11   b  is in a denatured state. The cell adhesion inhibitory region  11   b  also can be referred to as, for example, a region that has been irradiated with the light L. The cell adhesion inhibitory region  11   b  is a region where adhesiveness to cells is deteriorated as compared to that in the cell adhesion region  11   a , for example. Specifically, for example, the number of cells adhering to the cell adhesion inhibitory region  11   b  per unit area is reduced by at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% and preferably by 100% as compared to the number of cells adhering to the cell adhesion regions  11   a  per unit area. The numbers of adhering cells per unit area in the respective regions are determined by, for example, tests conducted under the same conditions except for the state of the cell culture base. In the tests, inducted pluripotent stem cells (iPS cells) are preferably used. In this case, culture conditions used in the tests are conditions under which the iPS cells remain undifferentiated. In the present embodiment, the cell culture base is denatured indirectly through light irradiation, whereby the adhesion capacity thereof with cells is deteriorated. Thus, the cell adhesion inhibitory region  11   b  contains the cell culture base in a denatured state. It is to be noted, however, that the present invention is not limited thereto, and the cell culture base may be such that the adhesion with cells is inhibited when it is in an undenatured state and the adhesion capacity thereof with cells is improved when it is denatured indirectly through light irradiation. In this case, the cell adhesion inhibitory region  11   b  contains the cell culture base in an undenatured state. Also, the cell culture base inhibits the cell adhesion in the absence of light irradiation. 
     Then, in the processing method according to the present embodiment, the culture tool  100  including the cell adhesion region  11   a  and the cell adhesion inhibitory region  11   b  is produced, as shown in  FIG.  2 E . In the production method of the present embodiment, the cell culture base is adherable to cells when it is in an undenatured state. Accordingly, in the inhibitory region forming step, light L is applied to a region of the photothermal conversion layer  13  adjacent to a region for forming a cell adhesion inhibitory region  11   b . It is to be noted, however, that the present invention is not limited thereto, and in the inhibitory region forming step, light L may be applied to a region of the photothermal conversion layer  13  adjacent to a region for forming a cell adhesion region  11   a . In this case, the cell culture base is adherable to cells when it is in a denatured state. 
     In the present embodiment, light energy can be efficiently converted into thermal energy by using the photothermal conversion layer  13 . Accordingly, in the present embodiment, the cell culture base in a region of the cell culture base layer  11  adjacent to the region of the photothermal conversion layer  13  irradiated with light L can be efficiently denatured. Thus, a processing apparatus according to the present invention to be described below can control a region to which cells can adhere in the culture tool  100  of the present embodiment. 
     Second Embodiment 
     The present embodiment relates to an example of a processing apparatus.  FIG.  3    shows an example of the structure of a processing apparatus according to the present embodiment.  FIG.  3    is a perspective view showing an example of the structure of the processing apparatus of the present embodiment. As shown in  FIG.  3   , a processing apparatus  200  of the present embodiment includes a laser irradiation unit  21  and a control unit  22 . The laser irradiation unit  21  includes a laser emission section  21   a , an optical fiber  21   b , and a laser source  21   c . The control unit  22  is connected to the laser irradiation unit  21 , more specifically, to the laser emission section  21   a  and the laser source  21   c  of the laser irradiation unit  21 . 
     Although the laser irradiation unit  21  includes the laser emission section  21   a , the optical fiber  21   b , and the laser source  21   c  in the processing apparatus  200  of the present embodiment, the laser irradiation unit  21  is not limited thereto as long as it is capable of applying a laser to the photothermal conversion layer  13  of the culture tool  100 . The laser irradiation unit  21  may be configured such that, for example, it includes the laser source  21   c  and applies a laser directly from the laser source  21   c  to the photothermal conversion layer  13  of the culture tool  100 . In the case where a laser from the laser source  21   c  is guided to the laser emission section  21   a , the laser may be guided using, instead of the optical fiber  21   b , a light guide unit such as a mirror or a micro electro-mechanical system (MEMS). However, the optical fiber  21   b  is preferable because it allows the laser source  21   c  to be arranged at any position, can reduce the size of the processing apparatus  200 , and also can reduce the weight of the processing apparatus  200  as compared with the case of using other light guide units. 
     In the processing apparatus  200  of the present embodiment, the laser emission section  21   a  is configured such that the irradiation position of a laser L emitted therefrom is movable by a laser moving unit (not shown). Alternatively, the laser irradiation unit  21  may be configured such that the irradiation position of the laser L is movable using a galvanometer mirror and an f6 lens, for example. Regarding the laser moving unit, reference can be made to the description thereon to be presented below. 
     The laser source  21   c  is, for example, a device that emits a continuous-wave laser or a pulsed laser. The laser source  21   c  may emit, for example, a high-frequency laser that has a long pulse width and approximates to a continuous wave. The output power of a laser emitted by the laser source  21   c  is not limited to particular values, and can be determined as appropriate according to, for example, the above-described absorption wavelength of the photothermal conversion molecules in the photothermal conversion layer  13 . The wavelength of a laser emitted by the laser source  21   c  is not limited to particular values, and the laser may be, for example, a laser with a wavelength of 405 nm, 450 nm, 520 nm, 532 nm, or 808 nm, such as a visible-light laser or an infrared laser. As a specific example, the laser source  331  may be a continuous-wave diode laser having a maximum output power of 5 W and a wavelength in the vicinity of 405 nm. 
     The control unit  22  controls the laser irradiation unit  21 .  FIG.  4    shows block diagrams showing the hardware structure of the control unit  22 . As shown in  FIG.  4   , the control unit  22  includes a central processing unit (CPU)  22   a , a main memory  22   b , an auxiliary storage device  22   c , a video codec  22   d , an I/O interface  22   e , and other components, and they are controlled by a controller (a system controller, an I/O controller, or the like)  22   f  and operate in cooperation with each other. These components are connected to each other via busses, for example. Examples of the auxiliary storage device  22   c  include storage units such as a flash memory and a hard disk drive. The video codec  22   d  includes: a graphics processing unit (GPU) configured to generate a screen to be displayed based on a drawing instruction received from the CPU  22   a  and to transmit the screen signals to, for example, a display device or the like provided outside the processing apparatus  200 ; and a video memory for temporarily storing data concerning the screen and the image. The input-output (I/O) interface  22   e  is a device that is communicably connected to and controls the laser emission section  21   a  and the laser source  21   c . The I/O interface  22   e  may include a servo driver (servo controller). The I/O interface  22   e  may be connected to, for example, an input device provided outside the processing apparatus  200 . Examples of the display device include monitors that output images (e.g., various image display devices such as a liquid crystal display (LCD) and a cathode ray tube (CRT) display). Examples of the input device include pointing devices such as a touch panel, a trackpad, and a mouse, a keyboard, and a push button, each operable by an operator with his/her fingers. The auxiliary storage device  22   c  stores a program executed by the control unit  22 . At the time of executing the program, the program is read into the main memory  22   b  and decoded by the CPU  22   a . The control section  22  controls each component according to the program. 
     The CPU  22   a  operates in cooperation with other components via the controller  22   f  (such as a system controller or an I/O controller) and is responsible for overall control of the processing apparatus  200 . In the control unit  22 , the CPU  22   a  executes the above-described program and other programs, and also, read and write various types of information, for example. Specifically, as shown in  FIG.  4 B , the CPU  22   a  functions as, for example, a setting section  221  and an irradiation control section  222 . Although the control unit  22  includes the CPU as an arithmetic unit, the control unit  22  may include another arithmetic unit such as a graphics processing unit (GPU) or an accelerated processing unit (APU), or may further include such a processing unit in combination with the CPU. The CPU  22   a  functions, for example, as respective sections, including an acquisition section and other sections, to be described below, and also, as respective sections other than a storage section in the third and fourth embodiments. 
     The main memory  22   b  is also referred to as a main storage device. When the CPU  22  executes processing, the main memory  22   b  reads in various operation programs, including the above-described program, stored in the auxiliary storage device  22   c  to be described below, for example. The CPU  22   a  then reads out data from the main memory  22   b , decodes the data, and executes the programs. The main memory  22   b  is a random-access memory (RAM), for example. Examples of the main memory  22   b  further include a read-only memory (ROM). 
     The auxiliary storage device  22   c  stores operation programs including the above-described program. The auxiliary storage device  22   c  includes, for example, a storage medium and a drive for reading and writing with respect to the storage medium. The storage medium is not limited to particular types of storage media, and for example, may be either a built-in or external storage medium, and may be a hard disk (HD), a Floppy® disk (FD), CD-ROM, CD-R, CD-RW, MO, DVD, a flash memory, a memory card, or the like. The drive is not limited to particular types of drives. The auxiliary storage device  22   c  may be, for example, a hard disk drive (HDD) in which the storage medium and the drive are integrated. When the control unit  22  includes the storage section, for example, the auxiliary storage device  22   c  serves as the storage section. 
     Next, a method for controlling the laser irradiation unit  21  by the control unit  22  of the processing apparatus  200  of the present embodiment will be described with reference to the flowchart of  FIG.  5   .  FIG.  5    is a flowchart illustrating an example of the processing (S 1  to S 2 ) executed by the control unit  22 . 
     First, in the step S 1 , the setting section  221  sets an irradiation region to be irradiated with a laser L in the culture tool  100  (setting step). Specifically, the setting section  221  associates each coordinate of the bottom surface  12   a  of the culture tool  100  with information on the presence or absence of irradiation of the laser L by the laser irradiation unit  21 . The coordinates can be acquired by, for example, setting a coordinate plane in a plane including the bottom surface  12   a . The coordinate plane can be set by, for example, setting an axis (X-axis) extending in one direction and an axis (Y-axis) extending in a direction orthogonal to the X-axis direction on the plane including the bottom surface  12   a . The center position of the coordinate plane can be set, for example, inside the bottom surface  12   a  or outside the bottom surface  12   a . The shape of the irradiation region set in the step S 1  is not limited to particular shapes and can be any shape. Although the present embodiment describes an illustrative example in which the setting section  221  directly sets an irradiation region, the setting section  221  may set a non-irradiation region not to be irradiated with the laser L to set an irradiation region indirectly or may set both an irradiation region and a non-irradiation region. 
     The irradiation region may be set in advance or may be set during use of the processing apparatus  200 , for example. In the case where the irradiation region is set in advance, information on the irradiation region (irradiation region information) is stored in the auxiliary storage device  22   c , for example. Thus, the setting section  221  sets the irradiation region to be irradiated with the laser L using the irradiation region information stored in the auxiliary storage device  22   c.    
     In the case where the irradiation shape is set during use of the processing apparatus  200 , the control unit  22  may include, for example, an acquisition section for acquiring irradiation region information in which the irradiation region is specified. In this case, the control method of the present embodiment includes, prior to the step S 1 , the step of acquiring irradiation region information in which the irradiation region is specified by the acquisition section. Then, in the step S 1 , the setting section  221  sets the irradiation region to be irradiated with the laser L based on the irradiation region information. 
     The acquisition section can acquire the irradiation region information by acquiring information in which the irradiation region is not specified and then specifying the irradiation region using the information in which the irradiation region is not specified. Examples of the information in which the irradiation region is not specified include: an image showing the whole or part of the surface of the cell culture tool (image data); and information on the cell culture tool, such as the size, volume, and material of the cell culture tool, the presence or absence of an adhesion treatment, and the like (identification information). 
     When the information in which the irradiation region is not specified is an image showing the whole or part of the surface of the cell culture tool, the acquisition section may acquire the irradiation region information by identifying an indistinct region formed in the cell culture tool in the above-described image and setting such a region as the irradiation region. As a specific example, in the case where an indistinct region caused by the meniscus that occurs when acquiring the image of the cell culture tool is set as the irradiation region, the processing apparatus  200  can set the irradiation region in the following manner, for example. The meniscus means a curved liquid surface formed at the boundary between the cell culture tool and liquid introduced into the cell culture tool. The indistinct region means, for example, a region in which, owing to the curved liquid surface formed at the boundary between the cell culture tool and the liquid introduced into the cell culture tool, a decrease in the contrast or an increase in the luminance value from the boundary toward the center of the cell culture tool is caused. As a specific example, in the case where the image includes a phase-contrast image acquired by a phase-contrast microscope, the indistinct region means a region in the image captured in the state where, owing to the curved liquid surface formed at the boundary between the cell culture tool and the liquid introduced into the cell culture tool, a phase shift from the boundary toward the center of the cell culture tool is present. 
     First, the acquisition section acquires an image that includes the irradiation region or an image that may include the irradiation region. The image can be acquired by an optical observation device such as a phase-contrast microscope, for example. The image includes the whole or part of the surface of the cell culture tool and preferably includes the whole of the surface of the cell culture tool. Next, the acquisition section extracts the indistinct region from the image. Specifically, the acquisition section compares the luminance value of each pixel of the image and/or the contrast of each pixel present in a region of a predetermined size with the threshold value, and determines whether the pixel or the region is affected by the meniscus. Then, when the luminance value is lower than or equal to the threshold value and/or the contrast is higher than the threshold value, the acquisition section determines that the pixel or the region is not affected by the meniscus, i.e., the pixel or the region forms a distinct region. Subsequently, the acquisition section associates the distinct region with information that the laser L should not be applied or does not associate the distinct region with information that the laser L should be applied. On the other hand, when the luminance value is higher than the threshold value and/or the contrast is lower than or equal to the threshold value, the acquisition section determines that the pixel or the region is affected by the meniscus, i.e., the pixel or the region forms an indistinct region. Then, the acquisition section associates the indistinct region with information that the laser L should be applied or does not associate the indistinct region with information that the laser L should not be applied. In this manner, the acquisition section can acquire irradiation region information in which the irradiation region is specified. The threshold value may be, for example, specified by a user or set in advance using an image acquired by capturing an image of the cell culture tool to which liquid has been introduced. 
     When the information in which the irradiation region is not specified is an image showing the whole or part of the surface of the cell culture tool, the acquisition section may identify the cell culture tool in the image including the cell culture tool and may acquire irradiation region information in which the irradiation region is specified from information on the thus-identified cell culture tool. As a specific example, in the case where an indistinct region caused by the meniscus that occurs when acquiring an image of the cell culture tool is set as the irradiation region, the processing apparatus  200  can set the irradiation region in the following manner, for example. First, the acquisition section acquires an image including the cell culture tool. The image can be acquired by an optical observation device such as a phase-contrast microscope, for example. The image includes the whole or part of the surface of the cell culture tool and preferably includes the whole of the surface of the cell culture tool. 
     Next, the acquisition section extracts a region where the cell culture tool is present from the image. Specifically, the acquisition section identifies (specifies) from the image the type of the cell culture tool included therein. The method of identifying the cell culture tool may be extracting information on the cell culture tool, such as the size, thickness, and material of the cell culture tool, from the image and then matching the information against a database in which various types of cell culture tools are associated with information on each of the cell culture tools. The database may be provided outside the processing apparatus  200 , or data may be stored in the auxiliary storage device  22   c  and used as a database. The above-described identification method may be performed by matching the image with images of various types of cell culture tools through image processing such as template matching. Then, the acquisition section identifies the cell culture tool specified by the above matching as the cell culture tool included in the image. Further, based on the thus-identified cell culture tool, the acquisition section extracts irradiation region information corresponding to the cell culture tool included in the image from a database in which various types of cell culture tools are associated with an irradiation region in which the irradiation region in each cell culture tool is specified. In the above-described manner, the acquisition section can acquire the irradiation region information from the image including the cell culture tool. Although the above example is directed to the case where the irradiation region information is acquired from an image including the cell culture tool using the features of the cell culture tool, the processing apparatus of the present invention is not limited thereto. When identification information (e.g., characters, graphics, and identifiers such as a QR Code®) that enables identification of the cell culture tool is provided (arranged) in the cell culture tool or a placement portion of the cell culture tool (e.g., a tool placement portion to be described below), the irradiation region information may be acquired using the identification information. In this case, the processing apparatus  200  further includes an identification information acquisition section for acquiring identification information of the cell culture tool, and the acquisition section can acquire irradiation region information associated with the cell culture tool based on the identification information of the cell culture tool. The identification information can be acquired, for example, using an optical observation device such as an optical microscope, as with the case of acquiring an image including the cell culture tool. The processing apparatus  200  may include a determination section for determining whether the image including the cell culture tool includes identification information. In this case, when the determination section determines that the image does not include identification information, the acquisition section acquires irradiation region information from the image including the cell culture tool. On the other hand, when the determination section determines that the image includes identification information, the identification information acquisition section acquires the identification information of the cell culture tool, and the acquisition section then acquires irradiation region information based on the identification information of the cell culture tool. 
     The irradiation region information may be, for example, an image in which the irradiation region is specified or information on a user-specified irradiation region. When the irradiation region is set using an image in which the irradiation region is specified, the setting section  221  can set the irradiation region by, for example, associating pixels satisfying a previously set condition in the image with information that the laser L should be applied and associating the remaining pixels with information that the laser L should not be applied. Alternatively, the setting section  221  may set the irradiation region by, for example, associating pixels satisfying a previously set condition in the image with information that the laser L should not be applied and associating the remaining pixels with information that the laser L should be applied. Also, the setting section  221  may set the irradiation region under conditions that are opposite to these conditions. The previously set condition may be, for example, a condition based on an irradiation region or a non-irradiation region in the image or a condition based on the contrast or luminance value of each pixel in the image. In this case, the setting section  221  can set the irradiation region by determining whether a region of interest is an irradiation region based on, for example, whether the contrast or luminance value of each pixel in the image satisfies the condition concerning the contrast or luminance value (e.g., the threshold value). 
     When the irradiation region is set using the information on a user-specified irradiation region, the setting section  221  can set the irradiation region by, for example, associating, in the above information, a region satisfying the previously set condition with information that the laser L should be applied and associating the remaining region with information that the laser L should not be applied. Alternatively, the setting section  221  may set the irradiation region by, for example, associating, in the above information, a region satisfying the previously set condition with information that the laser L should not be applied and associating the remaining region with information that the laser L should be applied. Also, the setting section  221  may set the irradiation region under conditions that are opposite to these conditions. The user specifies the irradiation region by, for example, enclosing the irradiation region. Thus, the previously set condition may be, for example, whether an enclosed region, i.e., a closed region, is formed in the user-specified irradiation region. In this case, the setting section  221  can set the irradiation region by determining whether a region of interest is the irradiation region based on, for example, whether there is a region forming a closed region in the information on the user-specified irradiation region. 
     Then, in the step S 2 , the irradiation control section  222  controls the laser irradiation unit  21  based on the irradiation region such that the laser irradiation unit  21  applies a laser to a region of the photothermal conversion layer  13  corresponding to the irradiation region in the culture tool  100 . Examples of the control of the laser irradiation unit  21  by the irradiation control section  222  include controlling the irradiation position of the laser L in the photothermal conversion layer  13  and ON/OFF switching of irradiation of the laser L. 
     When the irradiation control section  222  controls the irradiation position of the laser, the irradiation control section  222  can control the irradiation position of the laser by, for example, controlling the start, stop, and/or speed of the movement of a moving unit that can move the laser irradiation unit  21 . When the laser irradiation unit  21  includes a galvanometer mirror and an fo lens, the irradiation control section  222  can control the irradiation position of the laser by controlling the angle of the galvanometer mirror, for example. 
     When the irradiation control section  222  controls the ON/OFF of irradiation of the laser L, the irradiation control section  222  can control the ON/OFF of irradiation of the laser L by, for example, controlling the ON/OFF of laser light emission by the laser source  21   c . The irradiation control section  222  controls the ON/OFF of laser light emission based on, for example, each coordinate of the bottom surface  12   a  in the irradiation region set by the setting section  221  and information on the presence or absence of irradiation of the laser L by the laser irradiation unit  21 , associated with each coordinate. 
     The control unit  22  controls the irradiation of the photothermal conversion layer  13  of the culture tool  100  with the laser L by the laser irradiation unit  21  in the above-described manner, whereby the shape of a cell adhesion inhibitory region  11   b  formed on the cell culture base layer  11  of the culture tool  100  can be controlled. Although the processing apparatus  200  of the present embodiment is configured such that the control unit  22  is responsible for overall control of the processing apparatus  200 , the processing apparatus of the present invention is not limited thereto and may be configured such that a control unit for the laser irradiation unit  21 , such as a laser controller, is provided separately and the control unit for the laser irradiation unit  21  functions as the irradiation control section  222 . 
     The processing apparatus  200  of the present embodiment can easily control a region to which cells can adhere in a cell culture tool including a cell culture base layer and a photothermal conversion layer by performing laser irradiation in a controlled manner. 
     Third Embodiment 
     The present embodiment relates to another example of the processing apparatus.  FIG.  6    shows an example of the structure of a control unit  22  of a processing apparatus according to the present embodiment.  FIG.  6    is a block diagram showing an example of the structure of the control unit  22  of the processing apparatus of the present embodiment. As shown in  FIG.  6   , the control unit  22  of the processing apparatus of the present embodiment has the same structure as the control unit  22  of the processing apparatus of the second embodiment, except that it includes an acquisition section  223 , a dividing section  224 , and a position acquisition section  225  in addition to a setting section  221  and an irradiation control section  222 , and the above description regarding the structure of the control unit  22  in the second embodiment also applies to the control unit  22  of the present embodiment. In the processing apparatus of the present embodiment, a CPU  22   a  functions as the setting section  221 , the irradiation control section  222 , the acquisition section  223 , the dividing section  224 , and the position acquisition section  225 . 
     Next, a method for controlling a laser irradiation unit  21  by the control unit  22  of the processing apparatus of the present embodiment will be described using the flowchart of  FIG.  7    with reference to an illustrative example in which, in a culture tool  100 , a circular non-irradiation region (R n ) and the remaining irradiation region (R i ) shown in  FIG.  8 A  are subjected to laser irradiation by the processing apparatus of the present embodiment.  FIG.  7    is a flowchart illustrating an example of processing (S 1  to S 5 ) executed by the control unit  22 .  FIG.  8    shows schematic views illustrating a method for setting the irradiation region. 
     First, in the step S 3 , the acquisition section  223  acquires irradiation region information in which the irradiation region is specified (acquisition step). Specifically, the acquisition section  223  acquires an image of a bottom surface  12   a  of the culture tool  100  as shown in  FIG.  8 A , including an irradiation region (R i ) to be irradiated and non-irradiation region (R n ) not to be irradiated with a laser L by the laser irradiation unit  21 . The image can be acquired by, for example, importing data including the image from the outside of the processing apparatus. 
     Next, in the step S 1 , the setting section  221  sets the irradiation region based on the image. Specifically, in the image acquired by the acquisition section  223 , a region where the luminance value is either less than a predetermined value or greater than or equal to the predetermined value is set as the irradiation region R i . In the present embodiment, the predetermined value is, for example, a value at which the irradiation region R i  shown in gray and the non-irradiation region R n  shown in white are distinguishable from each other. Accordingly, as shown in  FIG.  8 A , the setting section  221  sets the gray region as the irradiation region R i  based on the predetermined value. As a result, the white region is indirectly set as the non-irradiation region R n . 
     In the step S 4 , as shown in  FIG.  8 B , the dividing section  224  divides the thus-set irradiation region R i  into segments based on an irradiation width W (processing width) of the laser. In the present embodiment, the dividing section  224  divides the irradiation region R i  into strip-shaped segments with their longitudinal direction extending in the left-right direction (hereinafter also referred to as “X-axis direction”) in  FIG.  8   . As a result, in the step S 4 , the segments (L 1  to L 13 ) of the irradiation region are formed. The length of each of L 1  to L 13  in the upper-lower direction (hereinafter also referred to as “Y-axis direction”) corresponds to the irradiation width W. The irradiation width W also can be referred to as, for example, the length of the spot diameter of the laser irradiation unit  21  in the Y-axis direction. When the dividing section  224  divides a portion that extends over both the irradiation region R i  and the non-irradiation region R n , for example, when providing L 4  to L 8 , the dividing section  224  divides the portion such that, of the resulting segments of the irradiation region, segments having the same coordinate in the Y-axis direction are considered as a single segment. Although the irradiation width W is set constant, the irradiation width W may vary. Although the width of the segments (L 1  to L 13 ) is set to be the same as the irradiation width W, it may be different from the irradiation width W. In the latter case, the width of the segments (L 1  to L 13 ) may be greater than the irradiation width W. 
     Next, in the step S 5 , the position information acquisition section  225  acquires the positions of the endpoints of the segments L 1  to L 13 . Specifically, the position information acquisition section  225  acquires the coordinates of the endpoints at both ends of the segments, as indicated with cross marks (x) in  FIG.  8 C . The coordinates are, for example, coordinates on the XY plane that is set based on the X-axis direction and the Y-axis direction. For the segments including the non-irradiation region R n , such as L 4  to L 8 , the position information acquisition section  225  acquires the coordinates of the boundaries between the irradiation region R i  and the non-irradiation region R n  as the laser ON/OFF switching positions, as indicated with circles (∘) in  FIG.  8 C . Then, the position information acquisition section  225  associates each of the segments L 1  to  13  with the coordinates of the corresponding endpoints and the laser ON/OFF switching positions. 
     Next, control of the laser irradiation unit  21  by the irradiation control section  222  in the step S 2  will be described with reference to  FIG.  9   .  FIG.  9    shows schematic views showing an example of the control of the laser irradiation unit  21  by the irradiation control section  222 .  FIG.  9 A  is a schematic view showing an example of the control for the whole culture tool  100 .  FIG.  9 B  is a schematic view showing an example of the control for L 4  and L 5 . 
     As shown in  FIG.  9 A , in the step S 2 , the irradiation control section  222  controls the laser irradiation unit  21  based on the segments L 1  to L 13  of the irradiation region and the coordinates of the corresponding endpoints and the laser ON/OFF switching positions such that the laser irradiation unit  21  applies the laser L to the corresponding regions of the photothermal conversion layer  13 . Specifically, the irradiation control section  222  controls the laser irradiation unit  21  so as to apply the laser L from the left endpoint toward the right endpoint of L 1 . At this time, the irradiation control section  222  also acquires the irradiation position (coordinates) of the laser L from the laser irradiation unit  21 . When irradiation of the laser L by the laser irradiation unit  21  is OFF, the irradiation control section  222  acquires, as the irradiation position of the laser L from the laser irradiation unit  21 , a virtual irradiation position based on the assumption that the laser irradiation unit  21  applies the laser L. The virtual irradiation position can be calculated from the coordinates of the segment and the moving speed of the laser. The irradiation control section  222  may determine whether the position of the laser irradiation unit  21  aligns with the position of the segment of interest. In the processing apparatus of the present embodiment, the laser irradiation unit  21  is movable by a laser moving unit. Accordingly, the irradiation control section  222  can control the position of the laser irradiation unit  21  by controlling the position of the laser moving unit. For this reason, the irradiation control section  222  acquires the position of the laser moving unit as the irradiation position of the laser L from the laser irradiation unit  21 . After the irradiation of L 1  with the laser L is completed, the irradiation control section  222  controls the laser irradiation unit  21  such that the laser irradiation unit  21  can apply the laser L to the right endpoint of L 2 . Then, the irradiation control section  222  controls the laser irradiation unit  21  so as to apply the laser L to the corresponding region of the photothermal conversion layer  13  by moving the laser from the right endpoint toward the left endpoint of L 2 . Similarly, the irradiation control section  222  controls the laser irradiation unit  21  so as to apply the laser L from the left endpoint toward the right endpoint to L 3 . 
     Subsequently, the irradiation control section  222  controls the laser irradiation unit  21  so as to apply the laser L from the right endpoint toward the left endpoint of L 4 . L 4  is set so as to extend over both the irradiation region R i  and the non-irradiation region R n . Thus, as shown in  FIG.  9 B , L 4  includes two laser ON/OFF switching positions. As described above, the irradiation control section  222  has acquired the irradiation positions of the laser L from the laser irradiation unit  21 . Accordingly, the irradiation control section  222  can control ON/OFF switching of irradiation of the laser L by the laser irradiation unit  21  based on whether the irradiation position of the laser L from the laser irradiation unit  21  coincides with the laser ON/OFF switching positions. Specifically, when the laser irradiation unit  21  is applying the laser L to a region that extends between the right endpoint and the circle on the right in L 4 , the irradiation control section  222  determines that the irradiation position of the laser L from the laser irradiation unit  21  does not coincide with the laser ON/OFF switching positions and thus does not control the laser ON/OFF switching by the laser irradiation unit  21 . On the other hand, when the laser L from the laser irradiation unit  21  reaches the position of the circle on the right in L 4 , the irradiation control section  222  determines that the irradiation position of the laser L from the laser irradiation unit  21  coincides with the laser ON/OFF switching position and thus controls the laser ON/OFF switching by the laser irradiation unit  21 . In this case, since the laser irradiation unit  21  is applying the laser L, the irradiation control section  222  controls the laser irradiation unit  21  so as to turn off the laser L. Further, when the laser L from the laser irradiation unit  21  reaches the position of the circle on the left in L 4 , the irradiation control section  222  determines that the irradiation position of the laser L from the laser irradiation unit  21  (the virtual irradiation position based on the assumption that the laser irradiation unit  21  applies the laser L) coincides with the laser ON/OFF switching position and thus controls the laser ON/OFF switching by the laser irradiation unit  21 . In this case, since the laser irradiation unit  21  is not applying the laser L, the irradiation control section  222  controls the laser irradiation unit  21  so as to turn on the laser L. Then, the irradiation control section  222  controls the laser irradiation unit  21  such that the laser irradiation unit  21  can apply the laser L to the left endpoint of L 4 . The irradiation control section  222  controls the laser irradiation unit  21  such that regions of the photothermal conversion layer  13  corresponding to L 5  to L 13  are irradiated with the laser L in the same manner as in the above. Although the laser L is scanned over the respective segments sequentially and in alternating directions in the present embodiment, the scanning direction of the laser L in the processing apparatus of the present invention is not limited thereto and the laser may be scanned in one direction. Although L 1  to L 13  are irradiated with the laser L in this order in the present embodiment, there is no particular limitation on the order of irradiating the segments with the laser L. 
     The processing apparatus  200  of the present embodiment can easily control a region to which cells can adhere in a cell culture tool including a cell culture base layer and a photothermal conversion layer by performing laser irradiation in a controlled manner. That is, the processing apparatus  200  of the present embodiment can easily control the shape of the cell adhesion region  11   a  of the culture tool  100  by controlling the irradiation region. Also, the processing apparatus of the present embodiment can divide the irradiation region into segments based on the irradiation width of the laser L and irradiate the segments with the laser L. The irradiation width of the laser L can be adjusted to any desired width. For example, a narrower irradiation width of the laser allows more precise control of the shape, and a broader irradiation width of the laser allows a larger area to be irradiated with the laser L in a shorter time. Accordingly, the processing apparatus of the present embodiment is superior in terms of formability of the cell adhesion region  11   a , for example. 
     Although the control unit  22  of the processing apparatus directly controls the laser irradiation unit  21  in the present embodiment, control of the laser irradiation unit  21  in the processing apparatus of the present invention is not limited thereto. As described above, when the processing apparatus includes a control unit for the laser irradiation unit  21  independently from the control unit  22 , the position information acquisition section  225  writes, to this control unit for the laser irradiation unit  21 , the segments L 1  to L 13  of the irradiation region and the coordinates of the corresponding endpoints and the laser ON/OFF switching positions associated with the segments L 1  to L 13 . Then, based on the written information, the control unit for the laser irradiation unit  21  controls the laser irradiation unit  21  so as to apply the laser L to the photothermal conversion layer  13  of the culture tool  100 . 
     In the present embodiment, the irradiation position of the laser (a portion to be irradiated in the cell culture tool) is moved (scanned) at a substantially constant speed for both the irradiation region R i  and the non-irradiation region R n . However, the present invention is not limited thereto, and the irradiation control section  222  may change the moving speed of the irradiation position such that the moving speed differs between the irradiation region R i  and the non-irradiation region R n . Specifically, as shown in  FIGS.  21 A and  21 B , the irradiation control section  22  may move the irradiation position of the laser at a substantially constant speed in the irradiation region R i , whereas the irradiation control section  22  may increase and/or decrease the moving speed of the irradiation position in the non-irradiation region R n . In the case where the irradiation control section  222  changes the moving speed of the irradiated position of the laser such that it differs between the irradiation region R i  and the non-irradiation region R n , processing can be performed more quickly while maintaining the formability of the cell adhesion region  11   a . Also, the irradiation control section  222  may increase and/or decrease the moving speed of the irradiation position of the laser in regions outside the cell culture tool  100 . 
     Although the dividing section  224  divides the irradiation region R i  and the non-irradiation region R n  into strip-shaped segments in the present embodiment, the present invention is not limited thereto and the dividing section  224  may divide the irradiation region R i  into segments with any desired shape. As a specific example, as shown in  FIG.  22 A , the dividing section  224  may divide the irradiation region R i  into segments with an approximately circular (e.g., oval, circular, or perfectly circular) shape or with a spiral shape. By dividing the irradiation region R i  in such a manner, the processing apparatus  200  can reduce the scanning distance of the laser and thus can perform processing more quickly. Although the dividing section  224  divides the irradiation region R i  and the non-irradiation region R n  altogether in the present embodiment, the present invention is not limited thereto and only the irradiation region R i  may be divided. As specific examples, as shown in  FIGS.  22 A and  22 B , the dividing section  224  may divide only the irradiation region R i  into strip-shaped segments or approximately circular segments. By dividing the irradiation region R i  in such a manner, the processing apparatus  200  can perform processing more quickly, for example, in the case where an indistinct region is caused by the meniscus as described above. 
     Fourth Embodiment 
     The present embodiment relates to still another example of the processing apparatus.  FIG.  10    shows the structure of a processing apparatus  300  according to the present embodiment.  FIG.  10    shows schematic views showing the structure of the processing apparatus  300  of the present embodiment.  FIG.  10 A  is a perspective view showing an example of the structure of the processing apparatus  300  of the present embodiment.  FIG.  10 B  is a block diagram showing an example of a control unit  22 . As shown in  FIG.  10 A , the processing apparatus  300  of the present embodiment includes a displacement meter  23  as a displacement measurement section, in addition to the structural components of the processing apparatus  200  of the second embodiment. The displacement meter  23  is attached to a laser emission section  21   a . Also, as shown in  FIG.  10 B , in the processing apparatus  300  of the present embodiment, the control unit  22  further includes a displacement adjustment section  226 , in addition to the structural components of the control unit  22  of the processing apparatus  200  of the second embodiment. Except for the above, the structure of the processing apparatus  300  of the present embodiment is the same as the structure of the processing apparatus  200  of the second embodiment, and the above description regarding the processing apparatus  200  also applies to the processing apparatus  300 . 
     The displacement meter  23  can measure the distance to the culture tool  100 . The measurement system employed by the displacement meter  23  may be, for example, optical, eddy-current, ultrasonic, or laser force measurement system. When the displacement meter  23  and the displacement adjustment section  226  are provided as in the processing apparatus  300  of the present embodiment, the displacement adjustment section  226  moves the position of the laser emission section  21   a  based on the distance (displacement) measured by the displacement meter  23 , whereby the laser L can be controlled so as to be focused on the photothermal conversion layer  13 . With this structure, the processing apparatus  300  of the present embodiment can reduce strain and distortion of the culture tool  100  and thus can apply a desired light energy to the photothermal conversion layer  13 . 
     The displacement meter  23  need only be capable of measuring the distance to the culture tool  100  as described above, and may be, for example, an optical observation device such as an optical microscope. In this case, the displacement meter  23  can measure the distance utilizing the focusing function on the bottom surface  12   a  of the culture tool  100 . Specifically, the displacement meter  23  can back-calculate the distance from the optical observation device to the bottom surface  12   a  of the culture tool  100  based on the set value of the optical system when the optical observation device is focused on the bottom surface  12   a . The displacement meter  23  measures the length in the height direction (the length in the direction orthogonal to the bottom surface  12   a ) to the culture tool  100 , for example. In the present embodiment, the displacement meter  23  is attached to the laser emission section  21   a  and moves together with the laser emission section  21 . However, the present invention is not limited thereto, and the displacement meter  23  may be arranged so as not to move together with the components of the laser irradiation unit  21 , such as the laser emission section  21   a . In this case, the displacement meter  23  is preferably arranged at a position where the displacement meter  23  does not moves together with the laser irradiation unit  21  in the height direction whereas it moves together with the laser irradiation unit  21  in a region where the position in the height direction does not change or in the X- and Y-axis directions. With this structure, the displacement meter  23  can measure the height to the culture tool  100  from a fixed position in the height direction regardless of the position of the laser irradiation unit  21 . 
     Next, a processing method using the processing apparatus  300  of the present embodiment will be described with reference to the flowchart of  FIG.  11   .  FIG.  11    is a flowchart illustrating an example of processing (S 1 , S 2 , S 6 , and S 7 ) performed by the processing apparatus  300 . 
     First, in the step S 6 , the laser emission section  21   a  and the displacement meter  23  are placed below the bottom surface  12   a  of the culture tool  100 . Preferably, they are placed below a central portion of the culture tool  100 . Then, in the step S 6 , the distance, specifically the distance in the height direction, to the bottom surface  12   a  of the culture tool  100  is measured using the displacement meter  23 . The displacement meter  23  is attached to the laser emission section  21   a . Thus, in the step S 6 , taking the positional relationship between the laser emission section  21   a  and the displacement meter  23  into consideration, the height from the laser emission section  21   a  to the bottom surface  12   a  of the culture tool  100  is calculated based on the distance measured by the displacement meter  23 . 
     Next, the step S 1  is performed in the same manner as the step S 1  in the second embodiment. 
     In the step S 7 , based on the distance acquired in the step S 6 , the displacement adjustment section  226  adjusts the position, specifically, the position in the height direction, of the laser irradiation unit  21 . Specifically, the displacement adjustment section  226  adjusts the position of the laser irradiation unit  21  by controlling the above-described laser moving unit such that the laser L is focused on the photothermal conversion layer  13  when the laser irradiation unit  21  applies the laser L to the photothermal conversion layer  13 . In the case where a reference value is set for the position of the laser irradiation unit  21  in the height direction, the displacement adjustment section  226  may adjust the position of the laser irradiation unit  21  in the height direction based on the reference value in the height direction and the distance acquired in the step S 6 . 
     Then, the step S 2  is performed in the same manner as the step S 2  in the second embodiment. 
     In the case where the photothermal conversion layer  13  converts the light energy of the laser L into thermal energy, it is preferable that the laser L is focused on the photothermal conversion layer  13 . The position of the bottom surface  12   a  varies depending on the manufacturing lot of the culture tool  100 , and the culture tool  100  may have a tilted or distorted bottom surface  12   a . In this case, the position of the photothermal conversion layer  13  in the height direction is misaligned from the focal point of the laser L unless the height of the laser irradiation unit  21  is adjusted. As a result, the efficiency of converting the light energy of the laser L into thermal energy is reduced. According to the processing apparatus  300  of the present embodiment, the distance to the culture tool  100  is measured and the position of the laser irradiation unit  21  can be adjusted based on the thus-measured distance. This allows the laser L to be focused on the photothermal conversion layer  13 , whereby the culture tool  100  can be processed efficiently. 
     Fifth Embodiment 
     The present embodiment relates to still another example of the processing apparatus.  FIGS.  12  to  20    show an example of the structure of the processing apparatus according to the present embodiment.  FIG.  12    is a perspective view showing an example of the structure of the processing apparatus of the present embodiment.  FIG.  13    is a perspective view showing an example of the structure of a first region in the processing apparatus of the present embodiment.  FIG.  14    is a cross-sectional view of the first region as viewed along arrows I-I in  FIG.  12   .  FIG.  15 A  is an exploded perspective view showing an example of a tool placement portion in the processing apparatus of the present embodiment and  FIG.  15 B  is a cross-sectional view as viewed along arrows III-III in  FIG.  15 A .  FIG.  16    is a perspective view of the first region and a circulation unit in a state where an outer wall of the first region is removed.  FIG.  17    is a cross-sectional view of an upper part of the first region and the circulation unit as viewed along arrows II-II in  FIG.  12   .  FIG.  18 A  is a perspective view showing an example of the structure of a second region in the processing apparatus of the present embodiment and  FIG.  18 B  is a perspective view showing another example of the structure of the second region.  FIG.  19    is a block diagram showing an example of a control section of the processing apparatus of the present embodiment.  FIG.  20    is a perspective view showing another example of the structure of the processing apparatus of the present embodiment. 
     As shown in  FIG.  12   , a processing apparatus  400  of the present embodiment includes a first region  4 , a second region  5 , a third region  6 , and a circulation unit  7 , and the first region  4 , the second region  3 , and the third region  6  are arranged in succession in this order from the top toward the bottom. Although the processing apparatus  400  of the present embodiment includes the circulation unit  7 , the circulation unit  7  is an optional component and may or may not be present. The positional relationship among the first region  4 , the second region  5 , and the third region  6  need only be such that the first region  4  and the second region  5  are arranged in succession (adjacent to each other), and the third region  6  may be arranged at any position. For example, as shown in  FIG.  20   , the third region  6  may be arranged spaced apart from the first region  4  and the second region  5 . In the case where the third region  6  is arranged spaced apart from the first region  4  and the second region  5  as shown in  FIG.  20   , the processing apparatus  400  can also be referred to as a processing system, for example. The processing system may be a tabletop system, for example. The first region  4  is preferably arranged on the top of the second region  5 . When laser irradiation is performed from above a culture tool  100  using a laser irradiation unit  53  to be described below, it is necessary to place an emission aperture of a laser emission section  532  in a solution contained in the culture tool  100  in order to stabilize the focal point of the laser irradiation unit  53 . However, performing laser irradiation in this state causes a problem such as sticking or baking of components of the solution to the emission aperture of the laser emission section  532 , resulting in contamination of the emission aperture of the laser emission section  532 . Thus, by arranging the laser irradiation unit  53  as in the processing apparatus  400  of the present embodiment, it is possible to suppress the occurrence of contamination of the laser emission aperture of the laser irradiation unit  53  at the time of irradiating the photothermal conversion layer  13  in the culture tool  100  with the laser L using the laser irradiation unit  53  to be described below, for example. Accordingly, the processing apparatus  400  of the present invention can stabilize the output power of the laser emitted from the laser irradiation unit  53  and thus can efficiently process the culture tool  100 , for example. The material for forming each region is not limited to particular materials, and each region may be formed of, for example, a stainless steel plate, a rust-proof iron plate, and a resin plate that can be molded by vacuum molding, injection molding, compressed-air molding, or the like. The material for forming each region is preferably a non-light transmitting material because this allow a second imaging unit to be described below to capture clearer images of the inside of the culture tool  100 . The term “non-light transmitting” means that, for example, transmission of light having a wavelength that affects image capturing by the second imaging unit is suppressed. When the second imaging unit is a fluorescence microscope, the wavelength of the above-described light may be a wavelength corresponding to fluorescence to be detected, for example. Specific examples of the non-light transmitting material include the above-described materials for forming each region. The size and shape of each region are not limited to particular sizes and shapes, and can be set as appropriate according to the size and shape of each member (unit) to be placed in each region. In the processing apparatus  400  of the present embodiment, the first region  4  and the second region  5  are constituted by different housings, and the housings constituting the first region  4  and the second region  5  are arranged adjacent to each other. However, the present invention is not limited thereto, and the first region  4  and the second region  5  may be constituted by a single housing with the inner space of this housing being partitioned to provide the first region  4  and the second region  5 . By using different housings to constitute the first region  1  and the second region  5  as in the processing apparatus  400  of the present embodiment, maintenance of each member of the processing apparatus  400  can be performed easily and the processing apparatus  400  can be assembled easily, for example. 
     The first region  4  includes an opening  41   a  for operations on its front surface (on the front side in  FIG.  12   ) and an opening  41   b  for enabling maintenance on its side surface. The opening  41   a  is an opening for operations relating to processing of the culture tool  100  in a processing chamber in the first region  4 . The opening  41   b  is an opening for enabling maintenance of the processing chamber. The opening  41   a  preferably has a smaller opening area than the opening  41   b  because this allows the maintenance operations to be performed more easily, for example. There is no particular limitation on the size and the number of openings  41   a  and  41   b , and reference can be made to, for example, the size and the number of openings for operations and openings for enabling maintenance in safety cabinets. As a specific example, for the size and the number of openings  41   a  and  41   b , reference can be made to, for example, the standards for safety cabinets as specified in EN standards, namely, EN 12469:2000. The number of openings  41   b  is not limited and can be set freely. It is preferable to provide two or more openings  41   b  because, for example, maintenance can be performed more easily. The positions at which the openings  41   a  and  41   b  are arranged in the first region  4  are not limited to particular positions and can be set freely. Preferably, the openings  41   a  and  41   b  are arranged at different positions (e.g., on different side surfaces) in the first region  4 . Although the opening  41   b  is primarily intended to facilitate the maintenance in the processing apparatus  400  in the present embodiment, the opening  41   b  may also be used for other purposes. In the processing apparatus  400  of the present embodiment, for example, movement and the like of each member arranged inside can be observed through the opening  41   b . This allows a defective area to be observed directly when a trouble occurs in the processing apparatus  400 , and a user thus can consider countermeasures. 
     The front wall of the first region  4  is a double wall having an outer wall and an inner wall, and the opening  41   a  is opened/closed by moving a door  42   a  up/down along rails provided in a space between the outer wall and the inner wall. The opening  41   b  can be opened/closed by detaching/attaching a door  42   b  that covers the opening. The opening  41   b  is preferably sealed with the door  42   b  when processing the culture tool  100  in the processing chamber, for example. This can prevent, for example, the gas outside the processing apparatus  400  and the dust contained therein from flowing into the processing chamber. In the processing apparatus  400  of the present embodiment, the opening  41   a  and the door  42   a  thereof and the opening  41   b  and the door  42   b  thereof are optional components, and the processing apparatus  400  may or may not include them or may include either one of the openings and the door thereof. The wall of the first region  4  may be either a double wall or a single wall, and preferably is the former because the size of the processing apparatus  400  can be reduced by arranging other members inside the double wall. When the wall of the first region  1  is a single wall, the door  42   a  is arranged, for example, outside the first region  4 , as with the door  42   b . The opening and closing system of each door is not limited to particular systems. For example, the door may be a liftable door like the door  42   a , an externally attached door like the door  42   b , or any other type of door. The other type of door is, for example, a hinged double door, accordion door, or sliding door. The material for forming each door is not limited to particular materials. Examples thereof include the above-described materials for forming each region, and non-light transmitting materials are preferable. 
     As shown in  FIG.  13   , the inner space of the first region  4  of the processing apparatus  400  of the present embodiment is the processing chamber in which the culture tool  100  is processed. The processing chamber can be closed by closing the doors  42   a  and  42   b . In other words, the processing chamber is openable and closable. The processing chamber includes: an XY stage  43   a  and an arm  43   b  that collectively constitutes a suction/discharge moving unit; a suction/discharge unit  44 ; a light source  45 ; a drainage container placement portion  46   a ; a storage container placement portion  47   a ; a tool placement portion  48 ; and a collection container placement portion  49   a . Although the processing chamber includes the XY stage  43   a , the arm  43   b , the suction/discharge unit  44 , the light source  45 , the drainage container placement portion  46   a , the storage container placement portion  47   a , and the collection container placement portion  49   a  in the present embodiment, they are all optional components and may or may not be present. Alternatively, the processing chamber may include any one of them or two or more of them. The XY stage  43   a  is arranged on the bottom surface of the processing chamber so as to be movable in the directions of arrows X and Y The arm  43   b  including a pair of arms is arranged on the top of the XY stage  43   a . At the tip of one of the arms in the arm  43   b , the suction/discharge unit  44  is arranged with its suction/discharge port directed downward. At the tip of the other arm in the arm  43   b , the light source  45  is arranged so as to be capable of projecting (emitting) light downward. The drainage container placement portion  46   a , the storage container placement portion  47   a , the tool placement portion  48 , and the collection container placement portion  49   a  are arranged on the bottom surface of the processing chamber in this order along the moving direction of the XY stage  43   a , which is the direction of arrow X. A drainage container  46   b  including a tip member detachment unit  46   c  is placed in the drainage container placement portion  46   a , a storage container  47   b  is placed in the storage container placement portion  47   a , and a collection container  49   b  is placed in the collection container placement portion  49   a.    
     Although the XY stage  43   a  and the arm  43   b  are provided as the suction/discharge moving unit in the processing apparatus  400  of the present embodiment, the suction/discharge moving unit is not limited thereto. The suction/discharge moving unit need only be capable of moving the suction/discharge unit  44 , and a known moving unit can be used, for example. The moving direction of the suction/discharge moving unit is not limited to particular directions, and the suction/discharge moving unit may be, for example, movable in one direction (e.g., the direction of arrow Y), movable in two directions (e.g., the directions of arrows X and Y), or movable in three directions (e.g., the directions of arrows X, Y, and Z). In the case of two moving directions, the first direction need only be nonparallel to the second direction and is preferably orthogonal or substantially orthogonal to the second direction. In this case, a plane including the first direction and the second direction is preferably substantially parallel to the surface on which the tool placement portion  48  is arranged. In the case of three moving directions, the third direction need only intersect with a plane including the first direction and the second direction, for example, and is preferably orthogonal or substantially orthogonal to the plane including the first direction and the second direction. In the present embodiment, the XY stage  43   a  is a known stage capable of moving an object at high speed and precisely along the directions of arrows X and Y via, for example, a linear motor carriage. The arm  43   b  is extendable in the vertical direction (the direction of arrow Z). The arm  43   b , however, may be fixed. In the latter case, the suction/discharge moving unit is capable of moving the suction/discharge unit  44  only in a plane substantially parallel to the bottom surface of the processing chamber, i.e., only in the directions of arrows X and Y in  FIG.  13   . 
     The suction/discharge unit  44  sucks and discharges a solution, such as a culture medium, and cells in the culture tool  100 , for example. The suction/discharge unit  44  is used, for example, with a tip member to be described below attached to the suction/discharge port side thereof. The suction/discharge unit  44  is not limited to particular types of units, and a known suction/discharge unit can be used, for example. Specific examples thereof include electric pipette and electric syringe pumps. 
     The light source  45  emits light toward the tool placement portion  48  from above the tool placement portion  48 , for example. For example, when an optical microscope such as a phase-contrast microscope is used as a second imaging unit to be described below, it is preferable to use the light source  45  in combination. Light emitted by the light source  45  is visible light, for example. The light source  45  is not limited to particular types of light sources, and may be, for example, a known light source such as a xenon light source, a light emitting diode (LED) lighting device, or a laser diode (LD). In the present embodiment, the light source  45  is arranged in the arm  43   b  of the suction/discharge moving unit and moves synchronously with the suction/discharge unit  44 . However, the light source  45  may move asynchronously with the suction/discharge unit  44 . As a specific example, the light source  45  may be arranged in, for example, a light source moving unit that is different from the suction/discharge moving unit and capable of moving the light source  45 . In this case, a control unit  61  to be described below may include a light source movement control unit that controls the movement of the light source moving unit. For example, the above description regarding the moving direction of the suction/discharge unit also applies to the moving direction of the light source moving unit. 
     The drainage container placement portion  46   a  is a portion in which a drainage container  46   b  for draining a liquid sucked by the suction/discharge unit  44  can be placed. Although the drainage container  46   b  is placed in the drainage container placement portion  46   a  in the present embodiment, the drainage container  46   b  is an optional component and may or may not be present. In the present embodiment, the drainage container  46   b  is an open-topped box that has a wall extending upward on the storage container placement portion  47   a  side, and at the upper end of this wall, a wall (upper surface) including a tip member detachment unit  46   c  formed as a semicircular recess (notch) extends substantially parallel to the bottom surface of the processing chamber. Since the drainage container  46   b  can collect a tip member detached from the suction/discharge unit  44 , the drainage container  46   b  can also be referred to as, for example, a tip member collection container, and the drainage container placement portion  46   a  can also be referred to as a tip member collection container placement portion. Although the tip member detachment unit  46   c  is formed in the drainage container  46   b , it may be provided separately from the drainage container  46   b . The tip member detachment unit  46   c  may be arranged near the suction/discharge unit  44 , specifically, in the suction/discharge moving unit in which the suction/discharge unit  44  is arranged. 
     The storage container placement portion  47   a  is a portion in which the storage container  47   b  containing the tip member detachable from the suction/discharge unit  44  can be placed. Although the storage container  47   b  is placed in the storage container placement portion  47   a  in the present embodiment, the storage container  47   b  is an optional component and may or may not be present. The tip member is not limited to particular members and need only be capable of storing a liquid sucked by the suction/discharge unit  44  therein. For example, when the suction/discharge unit  44  is a pipette, the tip member may be a tip. The storage container  47   b  is, for example, a rack in which the tips are stored. The processing apparatus  400  of the present embodiment includes the tip member detachment unit  46   c  and the storage container placement portion  47   a , and with this structure, the movement at the time of sucking and discharging a solution, such as a culture medium, cells, and the like in the culture tool  100  can be simplified (shortened). 
     The collection container placement portion  49   a  is a portion in which the collection container  49   b  for collecting the sucked liquid containing cells collected by the suction/discharge unit  44  can be placed. Although the collection container  49   b  is placed in the collection container placement portion  49   a  in the present embodiment, the collection container  49   b  is an optional component and may or may not be present. The collection container  49   b  may be, for example, a culture vessel such as a known dish or a known flask. 
     In the present embodiment, the drainage container placement portion  46   a , the storage container placement portion  47   a , the tool placement portion  48 , and the collection container placement portion  49   a  are arranged in this order on the bottom surface of the processing chamber along the moving direction of the XY stage  43   a , which is the long axis direction (the direction of arrow X) in a plane substantially parallel to the surface on which the tool placement portion  48  is arranged, i.e., the bottom surface of the processing chamber. However, the respective placement portions need not be arranged along the long axis direction and need not be arranged in this order. In the present embodiment, the drainage container placement portion  46   a , the storage container placement portion  47   a , the tool placement portion  48 , and the collection container placement portion  49   a  are arranged in the above-described order. With this structure, for example, the suction/discharge unit  44  can move linearly, and the movement at the time of sucking and discharging a solution, such as a culture medium, cells, and the like in the culture tool  100  can be simplified (shortened). 
     As shown in  FIG.  14   , a first camera  80 , illumination lamps  81   a  and  81   b , and a germicidal lamp  82  are arranged above the opening  41   a  on the front wall of the processing chamber of the processing apparatus  400  of the present embodiment. The illumination lamps  81   a  and  81   b  are arranged on both sides of the first camera  80  in the direction of arrow X, and the germicidal lamp  82  is arranged above the first camera  80 . 
     Although the camera  80  is provided as a first imaging unit in the present embodiment, the first imaging unit is an optional component and may or may not be present. The first imaging unit is not limited to a camera and need only be capable of capturing images of the inside of the processing chamber. The first imaging unit is not limited to particular types of imaging units, and may be a known imaging unit such as a microscope or a camera, which may be used in combination with a solid-state imaging element (image sensor) such as a CCD or a complementary MOS (CMOS). Although the camera  80  is arranged on the front wall inside the processing chamber in the present embodiment, the position of the camera  80  is not limited to particular positions and can be set freely. The camera  80  is preferably arranged such that it can capture images of a wide range of area in the processing chamber. Specifically, in the case where the XY stage  43   a  and the arm  43   b , which collectively constitute the suction/discharge moving unit, and the suction/discharge unit  44  are arranged on the back side (the upper left side in  FIG.  13   ) of the tool placement portion  48  in the processing chamber as in the processing apparatus  400  of the present embodiment, it is preferable to arrange the camera  80  on the front side (the lower right side in  FIG.  13   ) of the processing chamber because this allows the camera  80  to capture images of a wide range of area in the processing chamber. The first imaging unit is preferably capable of capturing images at a plurality of magnifications (e.g., different magnifications), but may be capable of capturing images at one magnification. The magnification means an imaging magnification, for example. As a specific example, the camera  80  includes, for example, lenses with a plurality of magnifications (e.g., different magnifications). The first imaging unit may be capable of, for example, optical zooming or digital zooming. The processing apparatus  400  of the present embodiment includes the camera  80 , and this allows, for example, operations in the processing chamber to be checked, whereby the reliability of the operations is improved. The number of first imaging units arranged in the processing chamber is not limited, and may be one or more. 
     Although the illumination lamps  81   a  and  81   b  are provided as illumination units in the present embodiment, the illumination units are optional components and may or may not be present. The illumination unit is not limited to an illumination lamp and need only be capable of projecting light to (illuminating) the processing chamber. The illumination unit is not limited to particular types of illumination units, and for example, a known illuminating device such as a fluorescent lamp or a LED lamp can be used. Although the illumination lamps  81   a  and  81   b  are arranged on the front wall inside the processing chamber in the present embodiment, the positions of the illumination lamps  81   a  and  81   b  are not limited to particular positions and can be set freely. The illumination lamps  81   a  and  81   b  are preferably arranged such that they can project light to a wide range of area in the processing chamber, i.e., formation of shadows in the processing chamber is avoided as much as possible. Specifically, in the case where the XY stage  43   a  and the arm  43   b , which collectively constitute the suction/discharge moving unit, and the suction/discharge unit  44  are arranged on the back side (the upper left side in  FIG.  13   ) of the tool placement portion  48  in the processing chamber as in the processing apparatus  400  of the present embodiment, it is preferable to arrange the illumination lamps  81   a  and  81   b  on the front side (the lower right side in  FIG.  13   ) of the processing chamber because this allows the illumination lamps  81   a  and  81   b  to project light to a wide range of area in the processing chamber. The processing apparatus  400  of the present embodiment includes the illumination lamps  81   a  and  81   b , and this allows, for example, operations in the processing chamber to be checked, whereby the reliability of the operations is improved. The number of the illumination units arranged in the processing chamber is not limited, and may be one or more. 
     Although the germicidal lamp  82  is provided as a germicidal unit in the present embodiment, the germicidal unit is an optional component and may or may not be present. The germicidal unit is not limited to a germicidal lamp and need only be capable of disinfecting the inside of the processing chamber, especially a portion around the tool placement portion  48 . The germicidal unit is not limited to particular types of germicidal units, and for example, a known germicidal unit such as a germicidal lamp or an ultraviolet LED lamp can be used. Although the germicidal lamp  82  is arranged on the front wall inside the processing chamber in the present embodiment, the position of the germicidal lamp  82  is not limited to particular positions and can be set freely. For example, dust and the like outside the processing apparatus  400  flows in via the openings  41   a  and  41   b . Thus, the germicidal lamp  82  is preferably arranged such that it can disinfect areas near the openings  41   a  and  41   b . Specifically, in the case where the front wall of the processing chamber has the opening  41   a  as in the processing apparatus  400  of the present embodiment, the germicidal unit is preferably arranged above the opening  41   a  on the front wall of the processing chamber. Also, in the case where the side wall of the processing chamber has the opening  41   b  as in the processing apparatus  400  of the present embodiment, the germicidal unit is preferably arranged above the opening  41   b  on the side wall of the processing chamber. Moreover, in the case where the processing apparatus  400  includes the illumination unit and the germicidal unit, both of these units are preferably arranged on the same wall of the processing chamber, e.g., on the wall with the opening  41   a . In this case, it is preferable to arrange the germicidal unit above the illumination unit. The processing apparatus  400  of the present embodiment includes the germicidal lamp  82 , and this improves the cleanliness inside the processing chamber, for example. The number of germicidal units arranged in the processing chamber is not limited, and may be one or more. 
     For the size, shape, structure, and the like of the processing chamber in the first region  4  of the present embodiment, reference can be made to, for example, the size, shape, structure, and the like of safety cabinets, and as a specific example, reference can be made to the standards for safety cabinets as specified in EN 12469:2000 as described above. 
     As shown in  FIG.  15   , the tool placement portion  48  in the processing apparatus  400  of the present embodiment includes an upper lid  481  and a bottom portion  482 , and the upper lid  481  is detachably attached to the bottom portion  482 . In the present embodiment, the tool placement portion  48  is a box including the upper lid  481  and the bottom portion  482 , and the culture tool  100  is placed inside the box. However, the tool placement portion  48  is not limited thereto, and need only be configured such that: the culture tool  100  can be placed therein; the tool placement portion  48  is arranged in the processing chamber so as to be adjacent to the second region  5 ; and the adjacent portion (a bottom plate  486  in  FIG.  15   ) of the tool placement portion  48  to the second region  5  is capable of transmitting light. “Transmitting light” described above means, for example, allowing a laser emitted from by the laser irradiation unit  53  arranged in the second region  5  to pass therethrough. In the case where the second region  5  includes a second imaging unit to be described below, “transmitting light” means that the second imaging unit can capture images through the bottom plate  486 . The upper lid  481  has a light transmitting region  483  to allow the culture tool  100  to be irradiated with light from the light source  45 . The light transmitting region  483  is formed of a transparent glass plate or a transparent acrylic plate, for example. The bottom portion  482  includes a bottom wall  485  and the light-transmitting bottom plate  486 . The light-transmitting bottom plate  486  is formed of a transparent glass plate or a transparent acrylic plate, for example. The bottom plate  486  is adjacent to the second region  5 . Thus, it can also be said that the adjacent portion of the tool placement portion  48  to the second region  5 , i.e., the bottom plate  486 , forms a part of the wall of the processing chamber. The contact portion between the bottom plate  486  and the wall of the processing chamber is preferably sealed with a sealing member such as a gasket or a sealing material, for example. This can prevent, for example, the gas in the second region  5  and dust and the like contained therein from flowing into the tool placement portion  48  and the processing chamber. The bottom wall  485  includes four recesses  487  in which four culture tools  100  can be placed, respectively, and the side surface of each recess  487  has an inversely tapered shape that narrows from the inside toward the outside of the processing chamber (from the top toward the bottom in  FIG.  15 B ). Each recess  487  includes a protruding portion  488  protruding inward at its end on the bottom plate  486  side. The bottom end of the culture tool  100  comes in contact with the protruding portion  488 . Although the bottom wall  485  has four recesses  487  in the processing apparatus  400  of the present embodiment, the number of recesses  487  provided in the bottom wall  485  is not limited thereto, and can be set as appropriate according to the number of culture tools  100  to be placed. The size of each recess  487  can be set as appropriate according to the size of the culture tool  100  to be placed therein. With the above-described structure of the recess  487  in the tool placement portion  48  of the present embodiment, it becomes possible to place the culture tool  100  in the tool placement portion  48  regardless of the shape of the side surface of the culture tool  100 , for example. In the processing apparatus  400  of the present embodiment, the bottom wall  485  is formed in one piece by integrally forming its bottom surface wall and side walls. However, the bottom wall  485  is not limited thereto, and its bottom surface wall and side walls may be provided as separate members. When the bottom wall  485  is constituted by separate members, two or more types of members that differ from each other in, for example, the number and the size of recesses  487  provided therein can be prepared as members constituting the bottom surface wall of the bottom wall  485 . Thus, for example, according to the size and the number of culture tools  100 , the bottom surface wall of the bottom wall  485  can be replaced with the member with the size and the number of recesses suitable for the placement of the culture tools  100 , thereby allowing the culture tools  100  to be placed suitably. 
     The tool placement portion  48  may further include, for example, a temperature adjustment unit for adjusting the temperature of the culture tool  100 . The temperature adjustment unit allows culture conditions during culture of cells in the culture tool  100  to be kept constant, whereby damage to the cells during the cell culture can be reduced, for example. The temperature adjustment unit may be, for example, a heating unit such as a heater. 
     The tool placement portion  48  may further include, for example, a pH adjustment unit for adjusting the pH of a solution, such as a culture medium, in the culture tool  100 . When the tool placement portion  48  includes the pH adjustment unit, culture conditions during culture of cells in the culture tool  100  can be kept constant, whereby damage to the cells during the cell culture can be reduced, for example. The pH adjustment unit may be, for example, a carbon dioxide concentration adjustment unit, which specifically may be, for example, a carbon dioxide cylinder or a connector for connection with a carbon dioxide supply unit provided outside the processing apparatus  400 . 
     As shown in  FIGS.  16  and  17   , in the processing apparatus  400  of the present embodiment, the circulation unit  7  includes an intake section  71 , a circulation path  72 , a gas supply section  73 , and an exhaust section  74 . With this structure, the circulation unit  7  circulates the gas inside the processing chamber. 
     The intake section  71  sucks the gas in the processing chamber. The intake section  71  may suck gas outside the processing apparatus  400  instead of or in addition to the gas inside the processing chamber. In the present embodiment, the intake section  71  is arranged near (e.g., immediately below) the opening  41   a  of the processing chamber. Specifically, the intake section  71  has a plurality of openings (not shown, e.g., slits) formed on its upper surface and is arranged below the opening  41   a  such that these openings are in communication with the opening  41   a . By arranging the intake section  71  near the opening  41   a  of the processing chamber as described above, it becomes possible to prevent, for example, gas outside the processing apparatus  400  and dust and the like contained therein from flowing into the processing chamber when an operator opens the door  42   a  and performs operations in the processing chamber. The intake section  71  may be arranged at a position near the opening  41   b , instead of or in addition to a position near the opening  41   a . The intake section  71  may suck the gas inside the processing chamber using an air blowing unit such as a fan, for example. 
     The circulation path  72  connects the intake section  71  to the gas supply section  73  and the exhaust section  74 . In the present embodiment, the circulation path  72  is arranged in a space between the outer wall and the inner wall and on the top of the first region  4 . The circulation path  72  is a hollow tube, for example. One end of the circulation path  72  is in communication with the intake section  71 , and the other end of the circulation path  72  is in communication with the gas supply section  73  and the exhaust section  74 . By arranging the circulation path  72  in the space between the outer wall and the inner wall as in the processing apparatus  400  of the present embodiment, the size of the processing apparatus  400  can be reduced, for example. Although the circulation unit  7  includes the circulation path  72  in the present embodiment, the circulation path  72  may or may not be present. In the latter case, the intake section  71  is directly connected to the gas supply section  73  and the exhaust section  74 , for example. The circulation path  72  may feed the gas sucked by the intake section  71  to the gas supply section  73  and the exhaust section  74  using the air blowing unit such as a fan, for example. 
     When the circulation path  72  includes the air blowing unit, the air blowing unit may be arranged near the intake section  71 , the gas supply section  73 , or the exhaust section  74 , or may be arranged at any other position such as a central portion of any of these sections. However, it is preferable to arrange the air blowing unit near the intake section  71  because this improves suction by the intake section  71 , whereby the dust and the like can be more effectively prevented from flowing into the processing chamber, for example, as compared with the downflow caused by the gas supply section  73  to be described below. In the case where air blowing unit is arranged near the intake section  71 , the air blowing unit is preferably arranged in, for example, the second region  5  or the third region  6 . As a specific example, when the circulation path  72  further includes the air blowing unit in the processing apparatus  400  of the present embodiment, the air blowing unit is arranged on the front side (the lower left side in  FIG.  12   ), i.e., below the intake section  71 , in the second region  5  or the third region  6 . In this case, the circulation path  72  connects the intake section  71  to the intake side of the air blowing unit and also connects the blowing side of the air blowing unit to the gas supply section  73  and the exhaust section  74 . That is, the circulation path  72  is arranged so as to extend in the second region  5  or in the second region  5  and the third region  6 , in the space between the outer wall and the inner wall, and above the first region  4 . 
     The gas supply section  73  supplies part of the gas sucked by the intake section  71  into the processing chamber. In the present embodiment, the gas supply section  73  is in communication with the upper end of the first region  4  such that the gas sucked by the intake section  71  can be supplied into the processing chamber. The gas supply section  73  may supply gas into the processing chamber using the air blowing unit such as a fan, for example. The gas supply section  73  may also include, for example, a gas purification unit. In this case, gas supplied from the gas supply section  73  into the processing chamber passes through the gas purification unit. When the gas supply unit  73  includes the gas purification unit, it is possible to prevent the dust and the like from flowing into the processing chamber, for example. The gas purification unit may be, for example, a filter for collecting fine particulates, such as a high efficiency particulate air filter (HEPA filter) or an ultra-low penetration air filter (ULPA filter). In the processing apparatus  400  of the present embodiment, the upper part of the processing chamber is connected to the gas supply section  73 . With this structure, for example, downflow is caused by the gas blown from the gas supply section  73 , whereby dust and the like can be more effectively prevented from flowing into the processing chamber from the opening  41   a.    
     The exhaust section  74  discharges the remainder of the gas sucked by the intake section  71  to the outside of the processing chamber, specifically, to the outside of the processing apparatus  400 . In the present embodiment, the exhaust section  74  is placed at an upper end (the uppermost part) of the processing apparatus  400  such that the gas sucked by the intake section  71  can be discharged to the outside of the processing apparatus  400 . When the exhaust section  74  is provided in the uppermost part of the processing apparatus  400  as described above, the size of the processing apparatus  400  can be reduced and dust stirred up by the discharged gas can be prevented from flowing into the processing chamber, for example. The exhaust section  74  may discharge gas to the outside of the processing apparatus  400  using the air blowing unit such as a fan, for example. The exhaust section  74  may also include the gas purification unit, for example. In this case, gas discharged to the outside of the processing apparatus  400  from the exhaust section  74  passes through the gas purification unit. When the exhaust section  74  includes the gas purification unit, fine particles and the like generated in the processing chamber can be prevented from flowing out to the outside of the processing apparatus  400 , for example. 
     For the size, shape, structure, and the like of each component of the circulation unit  7  of the present embodiment, reference can be made to, for example, the size, shape, structure, and the like of safety cabinets, and as a specific example, reference can be made to the standards for safety cabinets as specified in EN 12469:2000 as described above. 
     As shown in  FIG.  18 A , in the processing apparatus  400  of the present embodiment, the second region  5  includes a second XY stage  51 , a microscope  52  having objective lenses  521   a  to  521   c  with three different magnifications, and a laser irradiation unit  53 . Although the processing apparatus  400  of the present embodiment includes the XY stage  51  and the microscope  52 , the XY stage  51  and the microscope  52  are optional components and the processing apparatus  400  may or may not include them or may include either one of them. The XY stage  51  is arranged on the bottom surface of the second region  5 , which is substantially parallel to the surface on which the tool placement portion  48  is arranged, i.e., the bottom surface of the processing chamber. In the XY stage  51 , two rails extending in the direction of arrow X are arranged on a common rail (moving path) extending in the direction of arrow Y so as to be movable on the common rail. Carriages  511   a  and  511   b  are arranged on the two rails extending in the direction of arrow X, respectively, so as to be movable on the rails. The laser irradiation unit  53  includes a laser source  531 , a laser emission section  532 , and an optical fiber  533 . On the top of the XY stage  51 , the microscope  52  is arranged on the carriage  511   b  with the objective lenses  521   a  to  521   c  facing upward (the direction of arrow Z), and the laser emission section  532  of the laser irradiation unit  53  is arranged on the carriage  511   a  with a laser emission aperture facing upward (the direction of arrow Z). The carriage  511   a  is movable up and down in the vertical direction (the direction of arrow Z). In the second region  5 , the laser source  531  is arranged on the bottom surface of a portion of the second region  5  that does not overlap the movable range of the XY stage  51 . One end of the optical fiber  533  is connected to the laser source  531 , and the other end is connected to the laser emission section  532 . 
     Although the XY stage  51  is provided as a laser moving unit and a second imaging moving unit in the processing apparatus  400  of the present embodiment, the laser moving unit and the second imaging moving unit are not limited thereto. The laser moving unit and the second imaging moving unit need only be capable of moving the laser irradiation unit  53  and a second imaging unit to be described below, respectively, and known moving units can be used, for example. In the present embodiment, the laser moving unit and the second imaging moving unit share the rail extending in the direction of arrow X (first direction). However, the laser moving unit and the second imaging moving unit may be independent from each other. As a specific example, as shown in  FIG.  18 B , the laser moving unit may be arranged as, for example, an XY stage  51   a  and the second imaging moving unit may be arranged as an XY stage  51   b  on the bottom surface of the second region  5 . The moving directions of the laser moving unit and the second imaging moving unit are not limited to particular directions, and these units may be, for example, movable in one direction (e.g., the direction of arrow Y), movable in two directions (e.g., the directions of arrows X and Y), or movable in three directions (e.g., the directions of arrows X, Y, and Z). In the case of two moving directions, the first direction need only be nonparallel to the second direction and is preferably orthogonal or substantially orthogonal to the second direction. In this case, a plane including the first direction and the second direction is preferably substantially parallel to the surface on which the tool placement portion  48  is arranged. In the case of three moving directions, the third direction need only intersect with a plane including the first direction and the second direction, for example, and is preferably orthogonal or substantially orthogonal to the plane including the first direction and the second direction. In the case where the laser moving unit is capable of moving the laser irradiation unit  53  in the direction substantially orthogonal to the surface on which the tool placement portion  48  is arranged, i.e., the bottom surface of the culture tool  100 , the laser moving unit can adjust the spot diameter to be described below, for example. In this case, the laser moving unit also serves as a spot diameter adjustment unit to be described below, for example. In the present embodiment, the XY stage  51  is a known stage capable of moving an object at high speed and precisely along the directions of arrows X and Y via, for example, a linear motor carriage. 
     Preferably, the laser moving unit and the second imaging moving unit are capable of moving the laser irradiation unit  53  and the second imaging moving unit, respectively, in the first direction (e.g., the direction of arrow Y in  FIG.  18 A ) in a plane substantially parallel to the surface on which the tool placement portion  48  is arranged, and the movement of the laser irradiation unit  53  by the laser moving unit in the first direction and the movement of the second imaging unit by the second imaging moving unit in the first direction are on the same straight line, as with the XY stage  51  according to the present embodiment. When the laser irradiation unit  53  and the second imaging unit move on the same straight line as described above, it is possible to reduce the number of times each unit is moved during processing such as, for example, capturing an image of the inside of the culture tool  100  by the second imaging unit and then processing the culture tool  100  by the laser irradiation unit  53 , whereby the time required for the processing can be reduced. Further, as with the XY stage  51  according to the present embodiment, it is preferable that: the laser moving unit includes the carriage  511   a  on which the laser irradiation unit  53  is arranged and the moving path (rail) that is arranged along the first direction and on which the carriage  511   a  moves; the second imaging moving unit includes the carriage  511   b  on which the second imaging unit is arranged and the moving path (rail) that is arranged along the first direction and on which the carriage  511   b  moves; and the moving path of the laser moving unit and the moving path of the second imaging unit are the same. With such a structure, it is possible to further reduce the number of times each unit is moved during the processing such as the image capturing by the second imaging unit and the processing performed thereafter by the laser irradiation unit  53 , whereby the time required for the processing can be further reduced. 
     Although the microscope  52  having the objective lenses  521   a  to  521   c  with three different magnifications is provided as the second imaging unit in the processing apparatus  400  of the present embodiment, the second imaging unit is not limited thereto and need only be capable of capturing images of the inside of the culture tool  100  placed in the tool placement portion  48 . The second imaging unit is not limited to particular types of imaging units, and may be a known imaging unit such as a microscope or a camera, which may be used in combination with a solid-state imaging element (image sensor) such as a CCD or a complementary MOS (CMOS). The microscope may be an optical microscope such as a phase-contrast microscope or a fluorescence microscope. The microscope may have functions of both the phase-contrast microscope and the fluorescence microscope, for example. The second imaging unit is preferably capable of capturing images at a plurality of magnifications, but may be capable of capturing images at one magnification. As a specific example, when the second imaging unit is a microscope, the microscope preferably includes objective lenses with a plurality of magnifications (e.g., different magnifications). In the present embodiment, the magnifications of the objective lenses  521   a  to  521   c  are, for example, 2, 4, and 8 times, respectively. The second imaging unit may be capable of, for example, optical zooming or digital zooming. When the first imaging unit and the second imaging unit are included as in the processing apparatus  400  of the present embodiment, the magnification of the second imaging unit is preferably higher than the magnification of the first imaging unit because this allows capturing of clearer images of the inside of the culture tool  100 . 
     In the processing apparatus  400  of the present embodiment, the laser irradiation unit  53  includes the laser source  531 , the laser emission section  532 , and the optical fiber  533 . However, the laser irradiation unit  53  is not limited thereto and need only be capable of applying a laser to the culture tool  100  placed in the tool placement portion  48 . The laser irradiation unit  53  may include the laser source  531 , for example, and the laser source  531  may directly apply a laser to the culture tool  100 . In the case where a laser from the laser source  531  is guided to the laser emission section  532 , the laser may be guided using, instead of the optical fiber  533 , a light guide unit such as a mirror or a micro electro mechanical system (MEMS). However, the optical fiber  533  is preferable because this allows the laser source  531  to be arranged at any desired position in the second region  5 , and for example, by arranging the laser source  531  in a portion in which other units such as the laser moving unit, the second imaging unit, and the second imaging moving unit are not arranged and that does not overlap the movable ranges of the other units, the size of the processing apparatus  400  can be reduced, and the weight of the processing apparatus  400  can be reduced as compared with the case of using other light guide units. 
     The laser source  531  is, for example, a device that emits a continuous-wave laser or a pulsed laser. The laser source  531  may emit, for example, a high-frequency laser that has a long pulse width and approximates to a continuous wave. The output power of a laser emitted by the laser source  531  is not limited to particular values, and can be determined as appropriate according to, for example, the photothermal conversion molecules in the photothermal conversion layer  13 . The wavelength of a laser emitted by the laser source  531  is not limited to particular values, and the laser may be, for example, a laser with a wavelength of 405 nm, 450 nm, 520 nm, 532 nm, or 808 nm, such as a visible-light laser or an infrared laser. When the culture tool  100  includes a laser absorption layer as described above, the laser source  531  generates, for example, a laser with a wavelength that can be absorbed by the laser absorption layer. As a specific example, the laser source  531  may be a continuous-wave diode laser having a maximum output power of 5 W and a wavelength in the vicinity of 405 nm. 
     When the laser irradiation unit  53  includes the laser emission section  532 , it is preferable that the laser moving unit moves the laser emission section  532 . When the laser moving unit moves the laser emission section  532  in the vertical direction (the direction of arrow Z in  FIG.  18   ), the laser emission section  532  is preferably moved in such a manner that the laser emission aperture of the laser emission section  532  does not come into contact with the bottom surface of the processing chamber, preferably the bottom surface of the tool placement portion  48 . As a specific example, the laser moving unit preferably moves the laser emission section  532  in such a manner that the laser emission aperture of the laser emission section  532  does not approach within 1 mm from the bottom surface of the tool placement portion  48 . When the laser moving unit moves the laser emission section  532  within such a range, it is possible to prevent, for example, swaying of a solution such as a culture medium in the culture tool  100  placed in the tool placement portion  48  caused by the contact between the laser emission section  532  and the bottom surface of the tool placement portion  48 . 
     In the present embodiment, the microscope  52  as the second imaging unit is arranged on the front side (the lower left side in  FIG.  18   ), and the laser irradiation unit  53  is arranged on the back side (the upper right side in  FIG.  18   ). However, the positional relationship between the second imaging unit and the laser irradiation unit  53  is not limited thereto, and the second imaging unit may be arranged on the back side and the laser irradiation unit  53  may be arranged on the front side, for example. In general, the second imaging unit such as a microscope has a larger volume than the laser irradiation unit  53 . Thus, when the tool placement portion  48  is arranged on the front side in the first region  4 , the size of the processing apparatus  400  can be reduced by arranging the second imaging unit on the back side and the laser irradiation unit  53  on the front side. 
     The processing apparatus  400  of the present embodiment may further include a spot diameter adjustment unit for adjusting the diameter of a spot formed in a portion to be irradiated with the laser in an object to be irradiated. The spot diameter means the beam diameter of a laser at a contact portion between the laser and the object to be irradiated. The spot diameter can be adjusted by, for example, switching at least one of a laser focusing lens and a collimator lens (collimation lens) of the laser irradiation unit  53  or changing the distance between the laser irradiation unit  53  and the object to be irradiated. In the former case, it is preferable that the laser irradiation unit  53  includes, for example, a plurality of lenses and that the spot diameter adjustment unit adjust the spot diameter through switching among the lenses. The plurality of lenses may be, for example, a plurality of focusing lenses, a plurality of collimator lenses, or a combination of at least one focusing lens and at least one collimator lens. The plurality of focusing lenses have focal lengths that differ from each other, for example. The plurality of collimator lenses have focal lengths that differ from each other, for example. Switching of the lenses may be performed, for example, manually or by a spot diameter adjustment control section to be described below. In the latter case, for example, a lens switching unit is provided, and switching of the lenses is performed by the lens switching unit. In the case where the spot diameter adjustment unit changes the distance, it is preferable that the spot diameter adjustment unit adjusts the spot diameter by adjusting the distance between the laser irradiation unit  53  and the object to be irradiated. The distance between the laser irradiation unit  53  and the object to be irradiated means, for example, the distance as measured in the direction substantially orthogonal to the surface on which the tool placement portion  48  is arranged, i.e., the bottom surface of the culture tool  100 . In the case where the laser irradiation unit  53  includes the laser emission section  532 , the distance between the laser irradiation unit  53  and the object to be irradiated means the distance between the laser emission section  532  and the object to be irradiated. The distance between the laser irradiation unit  53  and the object to be irradiated can be adjusted by, for example, the laser moving unit. As a specific example, by moving the laser irradiation unit  53  in the direction of arrow Z by the laser moving unit, the distance to the bottom of the culture tool  100  as the object to be irradiated can be adjusted. In the processing apparatus  400  of the present embodiment, the carriage  511   a  of the XY stage  51  as the laser moving unit can be moved up and down in the vertical direction (the direction of arrow Z). Thus, the laser moving unit in the present embodiment can also be referred to as, for example, a spot diameter adjustment unit. The spot diameter adjustment unit adjusts the spot diameter to, for example, make it smaller for processing in which a small spot diameter is preferable, for example. The spot diameter adjustment unit adjusts the spot diameter to make it larger for processing in which a large spot diameter is preferable, for example. The spot diameter is not limited to particular diameters, and can be set as appropriate according to the type of processing. When the processing apparatus  400  of the present embodiment includes the spot diameter adjustment unit, the spot diameter can be adjusted to an appropriate size at the time of processing the culture tool  100 , thereby enabling rapid processing, for example. Moreover, since the spot diameter can be adjusted to an appropriate size, the cell adhesion region  11   a  in the culture tool  100  can be formed with high formability, for example. 
     When the processing apparatus  400  of the present embodiment includes the spot diameter adjustment unit, it is preferable that a control section to be described below includes a spot diameter adjustment control section for controlling the adjustment of the spot diameter by the spot diameter adjustment unit. 
     In the processing apparatus  400  of the present embodiment, it is preferable that movement of gas is reduced between the processing chamber and the second region  5 . The movement of gas can be reduced by, for example, sealing the adjacent portion of the processing chamber to the second region  5  using the above-described sealing member such as a gasket or a sealant. By reducing the movement of gas as described above, it is possible to prevent, for example, dust contained in the gas from flowing into the processing chamber. 
     In the processing apparatus  400  of the present embodiment, the third region  6  includes a control unit  61  and a power supply section  62 . As shown in  FIG.  19   , the control unit  61  includes structural components similar to those of a personal computer, a server computer, a workstation, or the like. As shown in  FIG.  19   , the control unit  61  includes a central processing unit (CPU)  61   a , a main memory  61   b , an auxiliary storage device  61   c , a video codec  61   d , an I/O interface  61   e , and other components, and they are controlled by a controller (system controller, I/O controller, or the like)  61   f  and operate in cooperation with each other. Examples of the auxiliary storage device  61   c  include storage units such as a flash memory and a hard disk drive. The video codec  61   d  includes: a graphics processing unit (GPU) configured to generate a screen to be displayed based on a drawing instruction received from the CPU  61   a  and to transmit the screen signals to, for example, a display device or the like provided outside the processing apparatus  400 ; and a video memory for temporarily storing data concerning the screen and the image. The input-output (I/O) interface  61   e  is a device that is communicably connected to and controls the first XY stage  43   a  and the arm  43   b  (suction/discharge moving unit), the suction/discharge unit  44 , the camera  80  (first imaging unit), the second XY stage  61  (laser moving unit and second imaging moving unit), the microscope  52  (second imaging unit), the laser irradiation unit  53 , and the like. The I/O interface  61   e  may include a servo driver (servo controller). The I/O interface  61   e  may be connected to, for example, an input device provided outside the processing apparatus  400 . Examples of the display device include monitors that output images (e.g., various image display devices such as a liquid crystal display (LCD) and a cathode ray tube (CRT) display). Examples of the input device  8  include pointing devices such as a touch panel, a trackpad, and a mouse, a keyboard, and a push button, each operable by an operator with his/her fingers. 
     The program executed by the control unit  61  is stored in the auxiliary storage device  61   c . At the time of executing the program, the program is read into the main memory  61   b  and decoded by the CPU  61   a . Then, the control unit  61  controls each member according to the program. 
     The control unit  61  in the present embodiment includes, in addition to the structural components of the control unit  22  in the second embodiment, an irradiation control section, a suction/discharge control section, a first imaging control section, and a second imaging control section. However, the irradiation control suction, the suction/discharge control suction, the first imaging control suction, and the second imaging control suction are optional structural components and may or may not be present. In the processing apparatus  400  of the present embodiment, the control unit  61  has the functions of the irradiation control section, the suction/discharge control section, the first imaging control section, the second imaging control section, and the like. Thus, it is not necessary to provide separate control units for the respective members, thereby allowing the size reduction of the processing apparatus. It is to be noted, however, that the present invention is not limited thereto. For example, in order to reduce the load on the control unit  61 , each member may be provided with a control section, and the control unit  61  and the control sections of the respective members may work together to control the respective members. As specific examples, the laser emission and the like may be controlled by, for example, the control section provided in each member, and the movement of the laser irradiation unit  53  may be controlled by, for example, the control unit  61 . The control unit  61  may be composed of one semiconductor element, may be a chip in which a plurality of semiconductor elements are formed in one package, or may include a plurality of semiconductor elements provided on a substrate. 
     In the present embodiment, the irradiation control section controls laser irradiation performed by the laser irradiation unit  53  and the movement of the laser emission section  532  of the laser irradiation unit  53  performed by the laser moving unit, namely, the XY stage  51  and the carriage  511 . However, the laser control section may control either one of them. 
     In the present embodiment, the suction/discharge control section controls suction and discharge performed by the suction/discharge unit  44  and the movement of the suction/discharge unit  44  performed by the suction/discharge moving unit, namely, the XY stage  43   a  and the arm  43   b . However, the suction/discharge control unit may control either one of them. 
     In the present embodiment, the first imaging control section controls image capturing of the inside of the processing chamber performed by the first imaging unit, namely, the camera  80 . 
     In the present embodiment, the second imaging control section controls image capturing performed by the second imaging unit, namely, the microscope  52  and the movement of the microscope  52  performed by the second imaging moving unit, namely, the XY stage  51  and the carriage  511   b . However, the second imaging control section may control either one of them. 
     The power supply section  62  is not limited to particular power supplies, and a known power supply can be used. The power supply section  62  supplies electric power to, for example, members (units) powered by electricity, such as the laser irradiation unit  53 , the laser moving unit, the first imaging unit, the second imaging unit, the second imaging moving unit, the suction/discharge unit  44 , the suction/discharge moving unit, the circulation unit  7 , the illumination unit, the germicidal unit, and the control unit  61 . Thus, the power supply section  62  is electrically connected to, for example, the members (units) powered by electricity. The power supply section  62  supplies electric power at a voltage of 100 V, for example. This allows the processing apparatus  400  to be used in a typical electric power environment, for example. In the processing apparatus  400  of the present embodiment, the power supply section  62  is responsible for the entire power supply. Thus, it is not necessary to provide separate power supply sections for the respective members, thereby allowing the size reduction and weight reduction of the processing apparatus  400 , for example. It is to be noted, however, that the present invention is not limited thereto, and, for example, a dedicated power supply section may be provided for at least one of these units. 
     In the processing apparatus  400  of the present embodiment, a communication section (not shown) may be further provided in the third region  6 . The communication section has a function of transmitting/receiving data to/from external devices such as a personal computer and a mobile communication device or a function of connecting to the Internet or the like through, for example, wired or wireless means. The communication section may be an existing communication module, for example. By providing the communication section as described above, it becomes possible to connect the processing apparatus  400  to external devices, and this allows the processing apparatus  400  to be operated from the outside or to receive data from the outside, for example. Also, it becomes possible to browse data stored in the processing apparatus  400  through connection to the processing apparatus  400  from the outside, for example. 
     Next, processing of a culture tool using the processing apparatus  400  of the present embodiment will be described with reference to an illustrative example. 
     First, the germicidal lamp  82  is turned off, and the illumination lamps  81   a  and  81   b  are turned on. The first imaging control section activates the camera  80  to start capturing of an image of the inside of the processing chamber. The image of the inside of the processing chamber captured by the camera  80  is output to the display device via, for example, the control unit  61 . Next, the circulation unit  7  is activated to circulate the gas inside the processing chamber. Further, a user opens the door  42   a  of the opening  41   a , and places a culture tool  100  in the tool placement portion  48 . After placing the culture tool  100 , the operator closes the door  42   a  of the opening  41   a.    
     Next, the XY stage  51  and the carriage  511   b  are moved under the control of the second imaging control section, whereby the microscope  52  is moved to be located below the bottom surface of the culture tool  100 . Also, the XY stage  43   a  is moved under the control of the suction/discharge control section, whereby the light source  45  is moved to be located above the upper surface of the culture tool  100 , i.e., above the tool placement portion  48 . Then, focusing of the microscope  52  is performed such that the microscope  52  is focused on the bottom surface  12   a  of the culture tool  100 , and the distance to the culture tool  100  is measured. Focusing of the microscope  52  may be performed a plurality of times using, for example, the objective lenses  521   a  to  521   c  that differ from each other in magnification. The microscope  52  may capture images over time. In this case, the images captured over time by the microscope  52  may be, for example, phase-contrast microscope images captured by a phase-contrast microscope or fluorescence microscope images captured by a fluorescence microscope. The captured images are output to the display device via, for example, the control unit  61 . 
     When the user inputs, for example, information on a user-specified irradiation region using the input device, the control unit  61  sets an irradiation region based on the input information on the irradiation region in the same manner as the control unit in the second embodiment. Subsequently, the control unit  61  controls the laser irradiation unit  53  based on the irradiation region such that the laser irradiation unit  53  applies the laser L to a region of the photothermal conversion layer  13  corresponding to the irradiation region. 
     Thereafter, the user opens the door  42   a  of the opening  41   a  and takes out the culture tool  100  from the tool placement portion  48 . Thus, the culture tool  100  processed by the processing apparatus  400  of the present embodiment can be collected. 
     The processing apparatus  400  of the present embodiment can easily control a region to which cells can adhere in a cell culture tool including a cell culture base layer and a photothermal conversion layer in, for example, an aseptic condition or a clean space. 
     While the present invention has been described above with reference to exemplary embodiments, the present invention is by no means limited these embodiments. Various changes and modifications that may become apparent to those skilled in the art may be made in the configuration and specifics of the present invention without departing from the scope of the present invention. 
     This application claims priority from Japanese Patent Application No. 2020-044841 filed on Mar. 14, 2020. The entire disclosure of this Japanese patent application is incorporated herein by reference. 
     &lt;Supplementary Notes&gt; 
     Part or the whole of the exemplary embodiments and examples disclosed above can be described as in the following supplementary notes. It is to be noted, however, that the present invention is by no means limited thereto. 
     (Supplementary Note 1) 
     A processing apparatus for a cell culture tool, including: 
     a laser irradiation unit capable of applying a laser to a photothermal conversion layer of a cell culture tool including a cell culture base layer and the photothermal conversion layer; and 
     a control unit for controlling the laser irradiation unit, wherein 
     the control unit includes a setting section and an irradiation control section, 
     the setting section sets an irradiation region to be irradiated with the laser in the cell culture tool, and 
     the irradiation control section controls the laser irradiation unit based on the irradiation region such that the laser irradiation unit apples the laser to a corresponding region of the photothermal conversion layer. 
     (Supplementary Note 2) 
     The processing apparatus according to Supplementary Note 1, wherein 
     the control unit includes a dividing section, 
     the dividing section divides the irradiation region into segments based on an irradiation width of the laser, and 
     the irradiation control section controls the laser irradiation unit based on the respective segments of the irradiation region such that the laser irradiation unit applies the laser to corresponding regions of the photothermal conversion layer. 
     (Supplementary Note 3) 
     The processing apparatus according to Supplementary Note 2, wherein 
     the laser irradiation unit includes a position acquisition section, 
     the position acquisition section acquires positions of endpoints of the respective segments of the irradiation region and associates the positions with the respective segments, and 
     the irradiation control section controls the laser irradiation unit based on the respective segments and the positions of the endpoints of the respective segments such that the laser irradiation unit applies the laser to the corresponding regions of the photothermal conversion layer by moving the laser in a direction from one endpoint toward the other endpoint in each segment. 
     (Supplementary Note 4) 
     The processing apparatus according to Supplementary Note 3, wherein 
     the irradiation control section controls the laser irradiation unit based on the respective segments and the positions of the endpoints of the respective segments such that the laser irradiation unit applies the laser to the corresponding regions of the photothermal conversion layer by moving the laser in a direction from one endpoint toward the other endpoint in each segment, 
     a direction in which the laser is moved in a preceding segment is changed in a subsequent segment such that the laser is moved in a direction from the other endpoint toward the one endpoint of the preceding segment, and 
     the control in this manner is performed with respect to the entire irradiation region. 
     (Supplementary Note 5) 
     The processing apparatus according to Supplementary Note 3 or 4, wherein 
     the position acquisition section acquires laser ON/OFF switching positions for the respective segments of the irradiation region, and 
     the irradiation control section controls the laser irradiation unit such that: 
     based on the respective segments and the positions of the endpoints of the respective segments, the laser irradiation unit applies the laser to the corresponding regions of the photothermal conversion layer by moving the laser in a direction from one endpoint toward the other endpoint in each segment; and 
     based on the laser ON/OFF switching positions, the laser is turned on or turned off. 
     (Supplementary Note 6) 
     The processing apparatus according to any one of Supplementary Notes 2 to 5, wherein 
     the dividing section divides the irradiation region into approximately circular segments or spiral segments based on the irradiation width of the laser. 
     (Supplementary Note 7) 
     The processing apparatus according to any one of Supplementary Notes 1 to 6, wherein 
     the control unit includes an acquisition section, 
     the acquisition section acquires irradiation region information in which the irradiation region is specified, and 
     the setting section sets the irradiation region based on the irradiation region information. 
     (Supplementary Note 8) 
     The processing apparatus according to Supplementary Note 7, wherein 
     the irradiation region information includes an image in which the irradiation region is specified, and 
     the setting section sets the irradiation region based on a luminance value of the image in which the irradiation region is specified. 
     (Supplementary Note 9) 
     The processing apparatus according to Supplementary Note 8, wherein 
     the irradiation region information includes information on a user-specified irradiation region, and 
     the setting section sets the irradiation region based on the information on the user-specified irradiation region. 
     (Supplementary Note 10) 
     The processing apparatus according to any one of Supplementary Notes 1 to 6, wherein 
     the control unit includes an acquisition section, 
     the acquisition section acquires an image including the cell culture tool, and acquires irradiation region information in which the irradiation region is specified by extracting the irradiation region from the image, and 
     the setting section sets the irradiation region based on the irradiation region information. 
     (Supplementary Note 11) 
     The processing apparatus according to any one of Supplementary Notes 1 to 6, wherein 
     the control unit includes an acquisition section, 
     the acquisition section identifies the cell culture tool in the image, and acquires irradiation region information associated with the cell culture tool based on the thus-acquired identification information for the cell culture tool, and 
     the setting section sets the irradiation region based on the irradiation region information. 
     (Supplementary Note 12) 
     The processing apparatus according to any one of Supplementary Notes 1 to 6, wherein 
     the control unit includes an identification information acquisition section and an acquisition section, 
     the identification information acquisition section acquires identification information for the cell culture tool, 
     the acquisition section acquires irradiation region information associated with the cell culture tool based on the identification information for the cell culture tool, and 
     the setting section sets the irradiation region based on the irradiation region information. 
     (Supplementary Note 13) 
     The processing apparatus according to any one of Supplementary Notes 1 to 12, further including a displacement measurement section, wherein 
     the displacement measurement section is capable of measuring a distance to the cell culture tool. 
     (Supplementary Note 14) 
     The processing apparatus according to Supplementary Note 13, wherein 
     the control unit includes a displacement adjustment section, and 
     the displacement adjustment section adjusts a position of the laser irradiation unit based on the distance to the cell culture tool. 
     (Supplementary Note 15) 
     The processing apparatus according to any one of Supplementary Notes 1 to 14, further including a first region, a second region, and a third region, wherein 
     the first region and the second region are arranged in succession, 
     the first region is a processing chamber for processing a cell culture tool, 
     the processing chamber can be closed from the outside of the processing chamber and includes a tool placement portion for placing the cell culture tool, 
     the second region includes the laser irradiation unit, and the laser irradiation unit can apply a laser to the cell culture tool placed in the tool placement portion, 
     the third region includes the control unit, 
     the tool placement portion is arranged in the processing chamber so as to be adjacent to the second region, and 
     an adjacent portion of the tool placement portion to the second region is capable of transmitting light. 
     (Supplementary Note 16) 
     The processing apparatus according to Supplementary Note 15, wherein the processing chamber includes an opening and a door capable of opening and closing the opening. 
     (Supplementary Note 17) 
     The processing apparatus according to Supplementary Note 16, wherein the door does not transmit light. 
     (Supplementary Note 18) 
     The processing apparatus according to Supplementary Note 16 or 17, wherein 
     the processing chamber includes an opening for operations relating to processing of the cell culture tool in the processing chamber and an opening for enabling maintenance of the processing chamber, and 
     the opening for operations and the opening for enabling maintenance are provided at different positions in the processing chamber. 
     (Supplementary Note 19) 
     The processing apparatus according to Supplementary Note 18, wherein the opening for operations has a smaller opening area than the opening for enabling maintenance. 
     (Supplementary Note 20) 
     The processing apparatus according to Supplementary Note 18 or 19, wherein 
     the processing chamber further includes a germicidal unit capable of disinfecting the inside of the processing chamber, and 
     the germicidal unit is arranged on a side closer to the opening for operations in the processing chamber. 
     (Supplementary Note 21) 
     The processing apparatus according to any one of Supplementary Notes 15 to 20, further including a circulation unit for circulating gas in the processing chamber, wherein 
     the circulation unit includes:
         an intake section for sucking the gas inside the processing chamber;   a gas supply section for supplying part of the sucked gas into the processing chamber; and   an exhaust section for discharging the remainder of the sucked gas to the outside of the processing chamber.       

     (Supplementary Note 22) 
     The processing apparatus according to Supplementary Note 21, wherein the exhaust section is arranged in an uppermost part of the processing apparatus. 
     (Supplementary Note 23) 
     The processing apparatus according to Supplementary Note 21 or 22, wherein, in the processing apparatus according to any one of Supplementary Notes 16 to 20, the intake section is arranged near the opening of the processing chamber. 
     (Supplementary Note 24) 
     The processing apparatus according to any one of Supplementary Notes 21 to 23, wherein 
     the processing chamber includes an outer wall and an inner wall, and 
     a circulation path that connects the intake section to the gas supply section and to the exhaust section is arranged between the outer wall and the inner wall. 
     (Supplementary Note 25) 
     The processing apparatus according to any one of Supplementary Notes 15 to 24, wherein the processing chamber further includes an illumination unit capable of projecting light to the processing chamber. 
     (Supplementary Note 26) 
     The processing apparatus according to any one of Supplementary Notes 15 to 25, wherein movement of gas is reduced between the processing chamber and the second region. 
     (Supplementary Note 27) 
     The processing apparatus according to any one of Supplementary Notes 15 to 26, wherein 
     the processing chamber further includes a suction/discharge unit and a suction/discharge moving unit for moving the suction/discharge unit, and 
     the control unit includes a suction/discharge control section for controlling suction and discharge performed by the suction/discharge unit and movement of the suction/discharge unit performed by the suction/discharge moving unit. 
     (Supplementary Note 28) 
     The processing apparatus according to Supplementary Note 27, wherein 
     the processing chamber includes a drainage container placement portion in which a drainage container for draining a liquid sucked by the suction/discharge unit can be placed, and 
     the tool placement portion and the drainage container placement portion are arranged along a moving direction of the suction/discharge moving unit in a plane substantially parallel to a surface on which the cell culture tool is placed. 
     (Supplementary Note 29) 
     The processing apparatus according to Supplementary Note 27 or 28, wherein 
     the processing chamber includes: 
     a storage container placement portion in which a tip member storage container containing a tip member detachable from the suction/discharge unit can be placed, and 
     a tip member detachment unit for detaching the tip member from the suction/discharge unit. 
     (Supplementary Note 30) 
     The processing apparatus according to any one of Supplementary Notes 15 to 29, wherein 
     the processing chamber includes a first imaging unit capable of capturing images of the inside of the processing chamber, and 
     the control unit includes a first imaging control section for controlling image capturing of the inside of the processing chamber by the first imaging unit. 
     (Supplementary Note 31) 
     The processing apparatus according to any one of Supplementary Notes 15 to 30, wherein 
     the second region includes a second imaging unit capable of capturing images of the inside of the culture tool placed in the tool placement portion, and 
     the control unit includes a second imaging control section for controlling image capturing performed by the second imaging unit, and 
     the second imaging unit is capable of capturing images at a plurality of magnifications. 
     (Supplementary Note 32) 
     The processing apparatus according to any one of Supplementary Notes 15 to 31, wherein 
     the laser irradiation unit includes a laser source and a laser emission section, and 
     the laser source is arranged in a portion of the second region in which other units are not arranged. 
     (Supplementary Note 33) 
     The processing apparatus according to any one of Supplementary Notes 15 to 32, wherein 
     the second region includes:
         a second imaging unit capable of capturing images of the cell culture tool placed in the tool placement portion;   a laser moving unit for moving the laser irradiation unit; and   a second imaging moving unit for moving the second imaging unit,       

     the control unit includes:
         an irradiation control section for controlling laser irradiation performed by the laser irradiation unit and movement of the laser irradiation unit performed by the laser moving unit; and   a second imaging control section for controlling image capturing performed by the second imaging unit and movement of the second imaging unit performed by the second imaging moving unit,       

     the laser moving unit is capable of moving the laser irradiation unit in a first direction in a plane substantially parallel to the surface on which the tool placement portion is arranged, 
     the second imaging moving unit is capable of moving the second imaging unit in the first direction in the plane substantially parallel to the surface on which the tool placement portion is arranged, and 
     movement of the laser irradiation unit in the first direction by the laser moving unit and movement of the second imaging unit in the first direction by the second imaging moving unit are on the same straight line. 
     (Supplementary Note 34) 
     The processing apparatus according to Supplementary Note 33, wherein 
     the laser moving unit includes a carriage on which the laser irradiation unit is arranged and a moving path that is arranged along the first direction and on which the carriage moves, 
     the second imaging moving unit includes a carriage on which the second imaging unit is arranged and a moving path that is arranged along the first direction and on which the carriage moves, and 
     the moving path for the laser moving unit and the moving path for the second imaging moving unit are the same. 
     (Supplementary Note 35) 
     The processing apparatus according to Supplementary Note 33 or 34, wherein the laser moving unit is capable of moving the laser irradiation unit further in a direction substantially orthogonal to the surface on which the tool placement portion is arranged. 
     (Supplementary Note 36) 
     The processing apparatus according to any one of Supplementary Notes 33 to 35, wherein the laser moving unit is capable of moving the laser irradiation unit in a second direction substantially orthogonal to the first direction in the plane substantially parallel to the surface on which the tool placement portion is arranged. 
     (Supplementary Note 37) 
     The processing apparatus according to any one of Supplementary Notes 15 to 36, wherein 
     a bottom surface of the tool placement portion includes a recess for placing the cell culture tool, 
     the recess includes a protruding portion that protrudes inward at its end on a side closer to the second region, and 
     a side surface of the recess has an inversely tapered shape that narrows from the inside toward the outside of the processing chamber. 
     (Supplementary Note 38) 
     The processing apparatus according to any one of Supplementary Notes 1 to 37, further including a monitoring (imaging) unit capable of monitoring (imaging) the inside of the cell culture tool. 
     INDUSTRIAL APPLICABILITY 
     The processing apparatus according to the present invention can control a region to which cells can adhere in a cell culture tool including a cell culture base layer and a photothermal conversion layer. Therefore, the present invention is very useful, for example, in the fields of regenerative medicine and drug discovery.