Patent Publication Number: US-2003236208-A1

Title: Targeted chromosomal genomic alterations in plants using modified single stranded oligonucleotides

Description:
FIELD OF THE INVENTION  
       [0001] The technical field of the invention is oligonucleotide-directed repair or alteration of plant genetic information using novel chemically modified oligonucleotides.  
       BACKGROUND OF THE INVENTION  
       [0002] A number of methods have been developed specifically to alter the genomic information of plants. These methods generally include the use of vectors such as, for example, T-DNA, carrying nucleic acid sequences encoding partial or complete portions of a particular protein which is expressed in a cell or tissue to effect the alteration. The expression of the particular protein then results in the desired phenotype. See, for example, U.S. Pat. No. 4,459,355 which describes a method for transforming plants with a DNA vector and U.S. Pat. No. 5,188,642 which describes cloning or expression vectors containing a transgenic DNA sequence which when expressed in plants confers resistance to the herbicide glyphosate. The use of such transgene-containing vectors adds one or more exogenous copies of a gene in a usually random fashion at one or more integration sites of the plant&#39;s genome at some variable frequency. The introduced gene may be foreign or may be derived from the host plant. Any gene which was originally present in the genome, which may be, for example, a normal allelic variant, mutated, defective, and/or functional copy of the introduced gene, is retained in the genome of the host plant.  
       [0003] These methods of gene alteration are problematic in that complications which can compromise the vigor, productivity, yield, etc. of the plant may result. One such problem is that insertion of exogenous nucleic acid at random location(s) in the genome can have deleterious effects. The random nature of this insertion and/or the use of exogenous promoters can also cause the timing, location or strength of expression of the introduced transgene to be inappropriate or unpredictable. Another problem with such systems includes the addition of unnecessary and unwanted genetic material to the genome of the recipient, including, for example, T-DNA ends or other vector remnants, exogenous control sequences required to allow production of the transgene protein, which control sequences may be exogenous or native to the host plant and/or the transgene, and reporter genes or resistance markers. Such remnants and added sequences may have presently unrecognized consequences, for example, involving genetic rearrangements of the recipient genomes. In addition, concerns have been raised with consumption, especially by humans, of plants containing such exogenous genetic material.  
       [0004] More recently, simpler systems involving poly- or oligo-nucleotides have been described for use in the alteration of genomic DNA. These chimeric RNA-DNA oligonucleotides, requiring contiguous RNA and DNA bases in a double-stranded molecule folded by complementarity into a double hairpin conformation, have been shown to effect single basepair or frameshift alterations, for example, for mutation or repair of plant, animal or fungal genomes. See, for example, WO 99/07865 and U.S. Pat. No. 5,565,350. In the chimeric RNA-DNA oligonucleotide, an uninterrupted stretch of DNA bases within the molecule is required for sequence alteration of the targeted genome while the obligate RNA residues are involved in complex stability. Due to the length, backbone composition, and structural configuration of these chimeric RNA-DNA molecules, they are expensive to synthesize and difficult to purify. Moreover, if the RNA-containing strand of the chimeric RNA-DNA oligonucleotide is designed so as to direct gene alteration, a series of mutagenic reactions resulting in nonspecific base alteration can result. Such a result reduces the utility of such a molecule in methods designed for targeted gene alteration.  
       [0005] Alternatively, other oligo- or poly-nucleotides have been used which require a triplex forming, usually polypurine or polypyrimidine, structural domain which binds to a DNA helical duplex through Hoogsteen interactions between the major groove of the DNA duplex and the oligonucleotide. Such oligonucleotides may have an additional DNA reactive moiety, such as psoralen, covalently linked to the oligonucleotide. These reactive moieties function as effective intercalation agents, stabilize the formation of a triplex and can be mutagenic. Such agents may be required in order to stabilize the triplex forming domain of the oligonucleotide with the DNA double helix if the Hoogsteen interactions from the oligonucleotide/target base composition are insufficient. See, e.g., U.S. Pat. No. 5,422,251. The utility of these oligonucleotides for directing targeted gene alteration is compromised by a high frequency of nonspecific base changes.  
       [0006] In more recent work, the domain for altering a genome is linked or tethered to the triplex forming domain of the bi-functional oligonucleotide, adding an additional linking or tethering functional domain to the oligonucleotide. See, e.g., Culver et al.,  Nature Biotechnology  17: 989-93 (1999). Such chimeric or triplex forming molecules have distinct structural requirements for each of the different domains of the complete poly- or oligo-nucleotide in order to effect the desired genomic alteration in either episomal or chromosomal targets.  
       [0007] Other genes, e.g. CFTR, have been targeted by homologous recombination using duplex fragments having several hundred basepairs. See, e.g., Kunzelmann et al.,  Gene Ther.  3:859-867 (1996). Similar efforts to target genes by homologous recombination in plants using large fragments of DNA had some success. See Kempin et al.,  Nature  389:802-803 (1997). However, the efficiency and reproducibility of the published homologous recombination approach in plants has severely limited the widespread use of this method.  
       [0008] Earlier experiments to mutagenize an antibiotic resistance indicator gene by homologous recombination used an unmodified DNA oligonucleotide rather than larger fragments of DNA, wherein the oligonucleotide had no functional domains other than a region of complementary sequence to the target. See Campbell et al.,  New Biologist  1: 223-227 (1989). These experiments required large concentrations of the oligonucleotide, exhibited a very low frequency of episomal modification of a targeted exogenous plasmid gene not normally found in the cell and have not been reproduced. However, as shown in examples herein, we have observed that an unmodified DNA oligonucleotide can convert a base at low frequency which is detectable using the assay systems described herein.  
       [0009] Oligonucleotides designed for use in the targeted alteration of genetic information are significantly different from oligonucleotides designed for antisense approaches. For example, antisense oligonucleotides are perfectly complementary to and bind an mRNA strand in order to modify expression of a targeted mRNA and are used at high concentration. As a consequence, they are unable to produce a gene conversion event by either mutagenesis or repair of a defect in the chromosomal DNA of a host genome. Furthermore, the backbone chemical composition used in most oligonucleotides designed for use in antisense approaches renders them inactive as substrates for homologous pairing or mismatch repair enzymes and the high concentrations of oligonucleotide required for antisense applications can be toxic with some types of nucleotide modifications. In addition, antisense oligonucleotides must be complementary to the mRNA and therefore, may not be complementary to the other DNA strand or to genomic sequences that span the junction between intron sequence and exon sequence.  
       [0010] Artificial chromosomes can be useful for the screening purposes identified herein. These molecules are man-made linear or circular DNA molecules constructed from essential cis-acting DNA sequence elements that are responsible for the proper replication and partitioning of natural chromosomes (Murray et al., 1983). The essential elements are: (1) Autonomous Replication Sequences (ARS), (2) Centromeres, and (3) Telomeres.  
       [0011] Yeast artificial chromosomes (YACs) allow large segments of genomic DNA to be cloned and modified (Burke et al., Science 236:806; Peterson et al., Trends Genet. 13:61 (1997); Choi, et al., Nat. Genet., 4:117-223 (1993), Davies, et al., Biotechnology 11:911-914 (1993), Matsuura, et al., Hum. Mol. Genet., 5:451-459 (1996), Peterson et al., Proc. Natl. Acad. Sci., 93:6605-6609 (1996); and Schedl, et al., Cell, 86:71-82 (1996)). Other vectors also have been developed for the cloning of large segments of genomic DNA, including cosmids, and bacteriophage P1 (Sternberg et al., Proc. Natl. Acad. Sci. U.S.A., 87:103-107 (1990)). YACs have certain advantages over these alternative large capacity cloning vectors (Burke et al., Science, 236:806-812 (1987)). The maximum insert size is 35-30 kb for cosmids, and 100 kb for bacteriophage P1, both of which are much smaller than the maximal insert size for a YAC.  
       [0012] An alternative to YACs are cloning systems based on the  E. coli  fertility factor that have been developed to construct large genomic DNA insert libraries. They are bacterial artificial chromosomes (BACs) and P-1 derived artificial chromosomes (PACs) (Mejia et al., Genome Res. 7:179-186 (1997); Shizuya et al., Proc. Natl. Acad. Sci. 89:8794-8797 (1992); Ioannou et al., Nat. Genet., 6:84-89 (1994); Hosoda et al., Nucleic Acids Res. 18:3863 (1990)). BACs are based on the  E. coli  fertility plasmid (F factor); and PACs are based on the bacteriophage P1. These vectors propagate at a very low copy number (1-2 per cell) enabling genomic inserts up to 300 kb in size to be stably maintained in recombination deficient hosts. The PACs and BACs are circular DNA molecules that are readily isolated from the host genomic background by classical alkaline lysis (Birnboim et al., Nucleic Acids Res. 7:1513-1523 (1979)). In addition, BACs have been developed for transformation of plants with high-molecular weight DNA using the T-DNA system (Hamilton,  Gene  24:107-116 (1997); Frary &amp; Hamilton,  Transgenic Res.  10: 121-132 (2001)).  
       [0013] A need exists for simple, inexpensive oligonucleotides capable of producing targeted alteration of genetic material such as those described herein as well as methods to identify optimal oligonucleotides that accurately and efficiently alter target DNA.  
       SUMMARY OF THE INVENTION  
       [0014] Novel, modified single-stranded nucleic acid molecules that direct gene alteration in plants are identified and the efficiency of alteration is analyzed both in vitro using a cell-free extract assay and in vivo using a yeast system and a plant system. The alteration in an oligonucleotide of the invention may comprise an insertion, deletion, substitution, as well as any combination of these. Site specific alteration of DNA is not only useful for studying function of proteins in vivo, but it is also useful for creating plants with desired phenotypes, including, for example, environmental stress tolerance, improved nutritional value, herbicide resistance, disease resistance, modified oil production, modified starch production, and altered floral morphology including selective sterility. As described herein, oligonucleotides of the invention target directed specific gene alterations in genomic double-stranded DNA in cells. The target genomic DNA can be nuclear chromosomal DNA as well as plastid or mitochondrial chromosomal DNA. The target DNA can also be a transgene present in the plant cell, including, for example, a previously introduced T-DNA. For screening purposes, the target plant DNA can also be extrachromosomal DNA present in plant or non-plant cells in various forms including, e.g., mammalian artificial chromosomes (MACs), PACs from P-1 vectors, yeast artificial chromosomes (YACs), bacterial artificial chromosomes (BACs), plant artificial chromosomes (PLACs), as well as episomal DNA, including episomal DNA from an exogenous source such as a plasmid or recombinant vector. Many of these artificial chromosome constructs containing plant DNA can be obtained from a variety of sources, including, e.g., the Arabidopsis Biological Resource Center (ABRC) at the Ohio State University, and the Rice Genome Research Program at the MAFF DNA bank in Ibaraki, Japan. The target DNA may be transcriptionally silent or active. In a preferred embodiment, the target DNA to be altered is the non-transcribed strand of a genomic DNA duplex. In a more preferred embodiment, the target DNA to be altered is the non-transcribed strand of a transcribed gene of a genomic DNA duplex.  
       [0015] The low efficiency of targeted gene alteration obtained using unmodified DNA oligonucleotides is believed to be largely the result of degradation by nucleases present in the reaction mixture or the target cell. Although different modifications are known to have different effects on the nuclease resistance of oligonucleotides or stability of duplexes formed by such oligonucleotides (see, e.g., Koshkin et al.,  J. Am. Chem. Soc.,  120:13252-3), we have found that it is not possible to predict which of any particular known modification would be most useful for any given alteration event, including for the construction of gene alteration oligonucleotides, because of the interaction of different as yet unidentified proteins during the gene alteration event. Herein, a variety of nucleic acid analogs have been developed that increase the nuclease resistance of oligonucleotides that contain them, including, e.g., nucleotides containing phosphorothioate linkages or 2′-O-methyl analogs. We recently discovered that single-stranded DNA oligonucleotides modified to contain 2′-O-methyl RNA nucleotides or phosphorothioate linkages can enable specific alteration of genetic information at a higher level than either unmodified single-stranded DNA or a chimeric RNA/DNA molecule. See, for example, copending applications U.S. application Ser. No. 60/208,538, U.S. application Ser. No. 60/244,989, U.S. application Ser. No. 09/818,875, international application no. PCT/US01/09761 and Gamper et al.,  Nucleic Acids Research  28: 4332-4339 (2000), the disclosures of which are incorporated herein in their entirety by reference. We also found that additional nucleic acid analogs which increase the nuclease resistance of oligonucleotides that contain them, including, e.g., “locked nucleic acids” or “LNAs”, xylo-LNAs and L-ribo-LNAs; see, for example, Wengel &amp; Nielsen, WO 99/14226; Wengel, WO 00/56748; Wengel, WO 00/66604; and Jakobsen &amp; Koshkin, WO 01/25478 also allow specific targeted alteration of genetic information.  
       [0016] The assay allows for determining the optimum length of the oligonucleotide, optimum sequence of the oligonucleotide, optimum position of the mismatched base or bases, optimum chemical modification or modifications, optimum strand targeted for identifying and selecting the most efficient oligonucleotide for a particular gene alteration event by comparing to a control oligonucleotide. Control oligonucleotides may include a chimeric RNA-DNA double hairpin oligonucleotide directing the same gene alteration event, an oligonucleotide that matches its target completely, an oligonucleotide in which all linkages are phosphorothiolated, an oligonucleotide fully substituted with 2′-O-methyl analogs or an RNA oligonucleotide. Such control oligonucleotides either fail to direct a targeted alteration or do so at a lower efficiency as compared to the oligonucleotides of the invention. The assay further allows for determining the optimum position of a gene alteration event within an oligonucleotide, optimum concentration of the selected oligonucleotide for maximum alteration efficiency by systematically testing a range of concentrations, as well as optimization of either the source of cell extract by testing different plants or strains, or testing cells derived from different plants or strains, or plant cell lines. Using a series of single-stranded oligonucleotides, comprising all RNA or DNA residues and various mixtures of the two, several new structures are identified as viable molecules in nucleotide conversion to direct or repair a genomic mutagenic event. When extracts from mammalian, plant and fungal cells are used and are analyzed using a genetic readout assay in bacteria, single-stranded oligonucleotides having one of several modifications are found to be more active than a control RNA-DNA double hairpin chimera structure when evaluated using an in vitro gene repair assay. Similar results are also observed in vivo using yeast, mammalian and plant cells. Molecules containing various lengths of modified bases were found to possess greater activity than unmodified single-stranded DNA molecules.  
       DETAILED DESCRIPTION OF THE INVENTION  
       [0017] The present invention provides oligonucleotides having chemically modified, nuclease resistant residues, preferably at or near the termini of the oligonucleotides, and methods for their identification and use in targeted alteration of plant genetic material, including gene mutation, targeted gene repair and gene knockout. The oligonucleotides are preferably used for mismatch repair or alteration by changing at least one nucleic acid base, or for frameshift repair or alteration by addition or deletion of at least one nucleic acid base. The oligonucleotides of the invention direct any such alteration, including gene correction, gene repair or gene mutation and can be used, for example, to introduce a polymorphism or haplotype or to eliminate (“knockout”) a particular protein activity. For example, gene alterations that knockout a particular protein activity can be obtained using oligonucleotides designed to convert a codon in the coding region of the protein to a stop codon, thus prematurely terminating translation of the protein. Oligonucleotides that introduce stop codons in the open-reading-frame of the protein are one embodiment of the invention. Generally, oligonucleotides that introduce stop codons early in the open-reading-frame of the protein are preferred. If the open-reading-frame contains more than one methionine, oligonucleotides that introduce stop codons after the second methionine are preferred. Additionally, if the gene exhibits alternative splice sites, oligonucleotides that introduce stop codons in exons after the alternative splice site are preferred. The following table provides examples of codons that can be converted to stop codons by altering a single oligonucleotide. A skilled artisan could readily identify other codons that can be converted to stop codons by altering one, two or three of the base pairs in a given codon. Similarly, a skilled artisan could readily identify codons that can be converted to stop codons by a frameshift mutations that inserts or deletes one or two base pairs in the open-reading-frame. It is also understood that more than one stop codon can be generated in a single open-reading-frame and that these stop codons can be adjacent in the sequence or separated by intervening codons. Where more than one stop codon is introduced into a single open-reading-frame, such alterations can be generated by a single or multiple oligonucleotides and can be generated simultaneously or by sequential mutagenesis of the target nucleic acid.  
                                       Corresponding       Original codons*   stop codon                      G   GA (glycine),    A   GA (arginine),    C   GA (arginine), T   T   A   TGA       (leucine), T   C   A (serine), TG   T    (cysteine), TG   G           (tryptophan), TG   C    (cysteine)           A   AG (lysine),    G   AG (glutamate),    C   AG (glutamine), T   T   G   TAG       (leucine), T   C   G (serine), T   G   G (tryptophan), TA   T           (cysteine), TA   C    (tyrosine)           A   AA (lysine),    G   AA (glutamate),    C   AA (glutamine), T   T   A   TAA       (leucine), T   C   A (serine), TA   T    (cysteine), TA   C           (tyrosine)                          
 
       [0018] The oligonucleotides of the invention are designed as substrates for homologous pairing and repair enzymes and as such have a unique backbone composition that differs from chimeric RNA-DNA double hairpin oligonucleotides, antisense oligonucleotides, and/or other poly- or oligo-nucleotides used for altering genomic DNA, such as triplex forming oligonucleotides. The single-stranded oligo-nucleotides described herein are inexpensive to synthesize and easy to purify. In side-by-side comparisons, an optimized single-stranded oligonucleotide comprising modified residues as described herein is significantly more efficient than a chimeric RNA-DNA double hairpin oligonucleotide in directing a base substitution or frameshift mutation in a cell-free extract assay.  
       [0019] We have discovered that single-stranded oligonucleotides having a DNA domain surrounding the targeted base, with the domain preferably central to the poly- or oligo-nucleotide, and having at least one modified end, preferably at the 3′ terminal region, are able to alter a target genetic sequence and with an efficiency that is higher than chimeric RNA-DNA double hairpin oligonucleotides disclosed in U.S. Pat. No. 5,565,350. Preferred oligonucleotides of the invention have at least two modified bases on at least one of the termini, preferably the 3′ terminus of the oligonucleotide. Oligonucleotides of the invention can efficiently be used to introduce targeted alterations in a genetic sequence of DNA in the presence of human, animal, plant, fungal (including yeast) proteins and in cells of different types including, for example, plant cells, fungal cells including  S. cerevisiae, Ustillago maydis, Candida albicans,  and mammalian cells. Particularly preferred are cells and cell extracts derived from plants including, for example, experimental model plants such as  Chiamydomonas reinhardtii, Physcomitrella patens,  and  Arabidopsis thaliana  in addition to crop plants such as cauliflower ( Brassica oleracea ), artichoke ( Cynara scolymus ), fruits such as apples ( Malus,  e.g.  domesticus ), mangoes ( Mangifera,  e.g.  indica ), banana ( Musa,  e.g.  acuminata ), berries (such as currant,  Ribes,  e.g.  rubrum ), kiwifruit ( Actinidia , e.g.  chinensis ), grapes ( Vitis,  e.g.  vinifera ), bell peppers ( Capsicum,  e.g.  annuum ), cherries (such as the sweet cherry,  Prunus,  e.g.  avium ), cucumber ( Cucumis,  e.g.  sativus ), melons ( Cucumis,  e.g.  melo ), nuts (such as walnut,  Juglans,  e.g.  regia;  peanut,  Arachis hypogeae ), orange ( Citrus,  e.g.  maxima ), peach ( Prunus,  e.g.  persica ), pear ( Pyra,  e.g.  communis ), plum ( Prunus,  e.g.  domestica ), strawberry ( Fragaria,  e.g.  moschata  or  vesca ), tomato ( Lycopersicon,  e.g.  esculentum ); leaves and forage, such as alfalfa ( Medicago,  e.g.  sativa  or  truncatula ), cabbage (e.g.  Brassica oleracea ), endive ( Cichoreum,  e.g.  endivia ), leek ( Allium,  e.g.  porrum ), lettuce ( Lactuca,  e.g.  sativa ), spinach ( Spinacia,  e.g.  oleraceae ), tobacco ( Nicotiana,  e.g.  tabacum ); roots, such as arrowroot ( Maranta,  e.g.  arundinacea ), beet ( Beta,  e.g.  vulgaris ), carrot ( Daucus,  e.g.  carota ), cassava ( Manihot,  e.g.  esculenta ), turnip ( Brassica,  e.g.  rapa ), radish ( Raphanus,  e.g.  sativus ), yam ( Dioscorea,  e.g.  esculenta ), sweet potato ( Ipomoea batatas ); seeds, including oilseeds, such as beans ( Phaseolus,  e.g.  vulgaris ), pea ( Pisum,  e.g.  sativum ), soybean ( Glycine,  e.g.  max ), cowpea ( Vigna unguiculata ), mothbean ( Vigna aconitifolia ), wheat ( Triticum,  e.g.  aestivum ), sorghum ( Sorghum  e.g.  bicolor ), barley ( Hordeum,  e.g.  vulgare ), corn ( Zea,  e.g.  mays ), rice ( Oryza,  e.g.  sativa ), rapeseed ( Brassica napus ), millet (Panicum sp.), sunflower ( Helianthus annuus ), oats ( Avena sativa ), chickpea ( Cicer,  e.g.  arietinum ); tubers, such as kohlrabi ( Brassica,  e.g.  oleraceae ), potato ( Solanum,  e.g.  tuberosum ) and the like; fiber and wood plants, such as flax ( Linum  e.g.  usitatissimum ), cotton ( Gossypium  e.g.  hirsutum ), pine (Pinus sp.), oak (Quercus sp.), eucalyptus (Eucalyptus sp.), and the like and ornamental plants such as turfgrass ( Lolium,  e.g.  rigidum ), petunia ( Petunia,  e.g.  x hybrida ), hyacinth ( Hyacinthus orientalis ), carnation ( Dianthus  e.g.  caryophyllus ), delphinium ( Delphinium,  e.g.  ajacis ), Job&#39;s tears ( Coix lacryma - jobi ), snapdragon ( Antirrhinum majus ), poppy ( Papaver,  e.g.  nudicaule ), lilac ( Syringa,  e.g.  vulgaris ), hydrangea ( Hydrangea  e.g.  macrophylla ), roses (including Gallicas, Albas, Damasks, Damask Perpetuals, Centifolias, Chinas, Teas and Hybrid Teas) and ornamental goldenrods (e.g. Solidago spp.). Such plant cells can then be used to regenerate whole plants according to methods described herein or any method known in the art. The DNA domain of the oligonucleotides is preferably fully complementary to one strand of the gene target, except for the mismatch base or bases responsible for the gene alteration event(s). On either side of the preferably central DNA domain, the contiguous bases may be either RNA bases or, preferably, are primarily DNA bases. The central DNA domain is generally at least 8 nucleotides in length. The base(s) targeted for alteration in the most preferred embodiments are at least about 8, 9 or 10 bases from one end of the oligonucleotide.  
       [0020] According to certain embodiments, one or both of the termini of the oligonucleotides of the present invention comprise phosphorothioate modifications, LNA backbone (including LNA derivatives and analogs) modifications, or 2′-O-methyl base analogs, or any combination of these modifications. Oligonucleotides comprising 2′-O-methyl or LNA analogs are a mixed DNA/RNA polymer. The oligonucleotides of the invention are, however, single-stranded and are not designed to form a stable internal duplex structure within the oligonucleotide. The efficiency of gene alteration is surprisingly increased with oligonucleotides having internal complementary sequence comprising phosphorothioate modified bases as compared to 2′-O-methyl modifications. This result indicates that specific chemical interactions are involved between the converting oligonucleotide and the proteins involved in the conversion. The effect of other such chemical interactions to produce nuclease resistant termini using modifications other than LNA (including LNA derivatives or analogs), phosphorothioate linkages, or 2′-O-methyl analog incorporation into an oligonucleotide can not yet be predicted because the proteins involved in the alteration process and their particular chemical interaction with the oligonucleotide substituents are not yet known and cannot be predicted.  
       [0021] In the examples, oligonucleotides of defined sequence are provided for alteration of genes in particular plants. Provided the teachings of the instant application, one of skill in the art could readily design oligonucleotides to introduce analogous alterations in homologous genes from any plant. Furthermore, in the tables of these examples, the oligonucleotides of the invention are not limited to the particular sequences disclosed. The oligonucleotides of the invention include extensions of the appropriate sequence of the longer 120 base oligonucleotides which can be added base by base to the smallest disclosed oligonucleotides of 17 bases. Thus the oligonucleotides of the invention include for each correcting change, oligonucleotides of length 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, or 120 with further single-nucleotide additions up to the longest sequence disclosed. In some embodiments, longer nucleic acids of up to 240 bases which comprise the sequences disclosed herein may be used. Moreover, the oligonucleotides of the invention do not require a symmetrical extension on either side of the central DNA domain. Similarly, the oligonucleotides of the invention as disclosed in the various tables for alteration of particular plant genes contain phosphorothioate linkages, 2′-O-methyl analog or LNA (including LNA derivatives and analogs) or any combination of these modifications just as the assay oligonucleotides do.  
       [0022] The present invention, however, is not limited to oligonucleotides that contain any particular nuclease resistant modification. Oligonucleotides of the invention may be altered with any combination of additional LNAs (including LNA derivatives and analogs), phosphorothioate linkages or 2′-O-methyl analogs to maximize conversion efficiency. For oligonucleotides of the invention that are longer than about 17 to about 25 bases in length, internal as well as terminal region segments of the backbone may be altered. Alternatively, simple fold-back structures at each end of a oligonucleotide or appended end groups may be used in addition to a modified backbone for conferring additional nuclease resistance.  
       [0023] The different oligonucleotides of the present invention preferably contain more than one of the aforementioned backbone modifications at each end. In some embodiments, the backbone modifications are adjacent to one another. However, the optimal number and placement of backbone modifications for any individual oligonucleotide will vary with the length of the oligonucleotide and the particular type of backbone modification(s) that are used. If constructs of identical sequence having phosphorothioate linkages are compared, 2, 3, 4, 5, or 6 phosphorothioate linkages at each end are preferred. If constructs of identical sequence having 2′-O-methyl base analogs are compared, 1, 2, 3 or 4 analogs are preferred. The optimal number and type of backbone modifications for any particular oligo-nucleotide useful for altering target DNA may be determined empirically by comparing the alteration efficiency of the oligonucleotide comprising any combination of the modifications to a control molecule of comparable sequence using any of the assays described herein. The optimal position(s) for oligonucleotide modifications for a maximally efficient altering oligonucleotide can be determined by testing the various modifications as compared to control molecule of comparable sequence in one of the assays disclosed herein. In such assays, a control molecule includes, e.g., a completely 2′-O-methyl substituted molecule, a completely complementary oligonucleotide, or a chimeric RNA-DNA double hairpin.  
       [0024] Increasing the number of phosphorothioate linkages, LNAs or 2′-O-methyl bases beyond the preferred number generally decreases the gene repair activity of a 25 nucleotide long oligonucleotide. Based on analysis of the concentration of oligonucleotide present in the extract after different time periods of incubation, it is believed that the terminal modifications impart nuclease resistance to the oligo-nucleotide thereby allowing it to survive within the cellular environment. However, this may not be the only possible mechanism by which such modifications confer greater efficiency of conversion. For example, as disclosed herein, certain modifications to oligonucleotides confer a greater improvement to the efficiency of conversion than other modifications.  
       [0025] Efficiency of conversion is defined herein as the percentage of recovered substrate molecules that have undergone a conversion event. Depending on the nature of the target genetic material, e.g. the genome of a cell, efficiency could be represented as the proportion of cells or clones containing an extrachromosomal element that exhibit a particular phenotype. Alternatively, representative samples of the target genetic material can be sequenced to determine the percentage that have acquired the desire change. The oligonucleotides of the invention in different embodiments can alter DNA two, three, four, five, six, seven, eight, nine, ten, twelve, fifteen, twenty, thirty, and fifty or more fold more than control oligonucleotides. Such control oligonucleotides are oligonucleotides with fully phosphorothiolated linkages, oligonucleotides that are fully substituted with 2′-O-methyl analogs, a perfectly matched oligonucleotide that is fully complementary to a target sequence or a chimeric DNA-RNA double hairpin oligonucleotide such as disclosed in U.S. Pat. No. 5,565,350.  
       [0026] In addition, for a given oligonucleotide length, additional modifications interfere with the ability of the oligonucleotide to act in concert with the cellular recombination or repair enzyme machinery which is necessary and required to mediate a targeted substitution, addition or deletion event in DNA. For example, fully phosphorothiolated or fully 2-O-methylated molecules are inefficient in targeted gene alteration.  
       [0027] The oligonucleotides of the invention as optimized for the purpose of targeted alteration of genetic material, including gene knockout or repair, are different in structure from antisense oligo-nucleotides that may possess a similar mixed chemical composition backbone. The oligonucleotides of the invention differ from such antisense oligonucleotides in chemical composition, structure, sequence, and in their ability to alter genomic DNA. Significantly, antisense oligonucleotides fail to direct targeted gene alteration. The oligonucleotides of the invention may target either strand of DNA and can include any component of the genome including, for example, intron and exon sequences. The preferred embodiment of the invention is a modified oligonucleotide that binds to the non-transcribed strand of a genomic DNA duplex. In other words, the preferred oligonucleotides of the invention target the sense strand of the DNA, i.e. the oligonucleotides of the invention are complementary to the non-transcribed strand of the target duplex DNA. The sequence of the non-transcribed strand of a DNA duplex is found in the mRNA produced from that duplex, given that mRNA uses uracil-containing nucleotides in place of thymine-containing nucleotides.  
       [0028] Moreover, the initial observation that single-stranded oligonucleotides comprising these modifications and lacking any particular triplex forming domain have reproducibly enhanced gene alteration activity in a variety of assay systems as compared to a chimeric RNA-DNA double-stranded hairpin control or single-stranded oligonucleotides comprising other backbone modifications was surprising. The single-stranded molecules of the invention totally lack the complementary RNA binding structure that stabilizes a normal chimeric double-stranded hairpin of the type disclosed in U.S. Pat. No. 5,565,350 yet is more effective in producing targeted base conversion as compared to such a chimeric RNA-DNA double-stranded hairpin. In addition, the molecules of the invention lack any particular triplex forming domain involved in Hoogsteen interactions with the DNA double helix and required by other known oligonucleotides in other oligonucleotide-dependant gene conversion systems. Although the lack of these functional domains was expected to decrease the efficiency of an alteration in a sequence, just the opposite occurs: the efficiency of sequence alteration using the modified oligonucleotides of the invention is higher than the efficiency of sequence alteration using a chimeric RNA-DNA hairpin targeting the same sequence alteration. Moreover, the efficiency of sequence alteration or gene conversion directed by an unmodified oligonucleotide is many times lower as compared to a control chimeric RNA-DNA molecule or the modified oligonucleotides of the invention targeting the same sequence alteration. Similarly, molecules containing at least 3 2′-O-methyl base analogs are about four to five fold less efficient as compared to an oligonucleotide having the same number of phosphorothioate linkages.  
       [0029] The oligonucleotides of the present invention for alteration of a single base are about 17 to about 121 nucleotides in length, preferably about 17 to about 74 nucleotides in length. Most preferably, however, the oligonucleotides of the present invention are at least about 25 bases in length, unless there are self-dimerization structures within the oligonucleotide. If the oligonucleotide has such an unfavorable structure, lengths longer than 35 bases are preferred. Oligonucleotides with modified ends both shorter and longer than certain of the exemplified, modified oligonucleotides herein function as gene repair or gene knockout agents and are within the scope of the present invention.  
       [0030] Once an oligomer is chosen, it can be tested for its tendency to self-dimerize, since self-dimerization may result in reduced efficiency of alteration of genetic information. Checking for self-dimerization tendency can be accomplished manually or, preferably, using a software program. One such program is Oligo Analyzer 2.0, available through Integrated DNA Technologies (Coralville, Iowa 52241) (http://www.idtdna.com); this program is available for use on the world wide web at http://www.idtdna.com/program/oligoanalyzer/oligoanalyzer.asp.  
       [0031] For each oligonucleotide sequence input into the program, Oligo Analyzer 2.0 reports possible self-dimerized duplex forms, which are usually only partially duplexed, along with the free energy change associated with such self-dimerization. Delta G-values that are negative and large in magnitude, indicating strong self-dimerization potential, are automatically flagged by the software as “bad”. Another software program that analyzes oligomers for pair dimer formation is Primer Select from DNASTAR, Inc., 1228 S. Park St., Madison, Wis. 53715, Phone: (608) 258-7420 (http://www.dnastar.com/products/PrimerSelect.html).  
       [0032] If the sequence is subject to significant self-dimerization, the addition of further sequence flanking the “repair” nucleotide can improve gene correction frequency.  
       [0033] Generally, the oligonucleotides of the present invention are identical in sequence to one strand of the target DNA, which can be either strand of the target DNA, with the exception of one or more targeted bases positioned within the DNA domain of the oligonucleotide, and preferably toward the middle between the modified terminal regions. Preferably, the difference in sequence of the oligonucleotide as compared to the targeted genomic DNA is located at about the middle of the oligo-nucleotide sequence. In a preferred embodiment, the oligonucleotides of the invention are complementary to the non-transcribed strand of a duplex. In other words, the preferred oligonucleotides target the sense strand of the DNA, i.e. the oligonucleotides of the invention are preferably complementary to the strand of the target DNA the sequence of which is found in the mRNA.  
       [0034] The oligonucleotides of the invention can include more than a single base change. In an oligonucleotide that is about a 70-mer, with at least one modified residue incorporated on the ends, as disclosed herein, multiple bases can be simultaneously targeted for change. The target bases may be up to 27 nucleotides apart and may not be changed together in all resultant plasmids in all cases. There is a frequency distribution such that the closer the target bases are to each other in the central DNA domain within the oligonucleotides of the invention, the higher the frequency of change in a given cell. Target bases only two nucleotides apart are changed together in every case that has been analyzed. The farther apart the two target bases are, the less frequent the simultaneous change. Thus, oligonucleotides of the invention may be used to repair or alter multiple bases rather than just one single base. For example, in a 74-mer oligonucleotide having a central base targeted for change, a base change event up to about 27 nucleotides away can also be effected. The positions of the altering bases within the oligonucleotide can be optimized using any one of the assays described herein. Preferably, the altering bases are at least about 8 nucleotides from one end of the oligonucleotide.  
       [0035] The oligonucleotides of the present invention can be introduced into cells by any suitable means. According to certain preferred embodiments, the modified oligonucleotides may be used alone. Suitable means, however, include the use of polycations, cationic lipids, liposomes, polyethylenimine (PEI), electroporation, biolistics, microinjection and other methods known in the art to facilitate cellular uptake. For plant cells, biolistic or particle bombardment methods are typically used. According to certain preferred embodiments of the present invention, isolated plant cells are treated in culture according to the methods of the invention, to mutate or repair a target gene. Alternatively, plant target DNA may be modified in vitro or in another cell type, including for example, yeast or bacterial cells and then introduced into a plant cell as, for example, a T-DNA. Plant cells thus modified may be used to regenerate the whole organism as, for example, in a plant having a desired targeted genomic change. In other instances, targeted genomic alteration, including repair or mutagenesis, may take place in vivo following direct administration of the modified, single-stranded oligonucleotides of the invention to a subject.  
       [0036] The single-stranded, modified oligonucleotides of the present invention have numerous applications as gene repair, gene modification, or gene knockout agents. Such oligonucleotides may be advantageously used, for example, to introduce or correct multiple point mutations. Each mutation leads to the addition, deletion or substitution of at least one base pair. The methods of the present invention offer distinct advantages over other methods of altering the genetic makeup of an organism, in that only the individually targeted bases are altered. No additional foreign DNA sequences are added to the genetic complement of the organism. Such agents may, for example, be used to develop plants with improved traits by rationally changing the sequence of selected genes in isolated cells and using these modified cells to regenerate whole plants having the altered gene. See, e.g., U.S. Pat. No. 6,046,380 and U.S. Pat. No. 5,905,185 incorporated herein by reference. Such plants produced using the compositions of the invention lack additional undesirable selectable markers or other foreign DNA sequences. Targeted base pair substitution or frameshift mutations introduced by an oligonucleotide in the presence of a cell-free extract also provides a way to modify the sequence of extrachromosomal elements, including, for example, plasmids, cosmids and artificial chromosomes. The oligonucleotides of the invention also simplify the production of plants having particular modified or inactivated genes. Altered plant model systems such as those produced using the methods and oligonucleotides of the invention are invaluable in determining the function of a gene and in evaluating drugs. The oligonucleotides and methods of the present invention may also be used to introduce molecular markers, including, for example, SNPs, RFLPs, AFLPs and CAPs.  
       [0037] The purified oligonucleotide compositions may be formulated in accordance with routine procedures depending on the target. For example, purified oligonucleotide can be used directly in a standard reaction mixture to introduce alterations into targeted DNA in vitro or where cells are the target as a composition adapted for bathing cells in culture or for microinjection into cells in culture. The purified oligonucleotide compositions may also be provided on coated microbeads for biolistic delivery into plant cells. Where necessary, the composition may also include a solubilizing agent. Generally, the ingredients will be supplied either separately or mixed together in single-use form, for example, as a dry, lyophilized powder or water-free concentrate. In general, dosage required for efficient targeted gene alteration will range from about 0.001 to 50,000 μg/kg target tissue, preferably between 1 to 250 μg/kg, and most preferably at a concentration of between 30 and 60 micromolar.  
       [0038] For cell administration, direct injection into the nucleus, biolistic bombardment, electroporation, liposome transfer and calcium phosphate precipitation may be used. In yeast, lithium acetate or spheroplast transformation may also be used. In a preferred method, the administration is performed with a liposomal transfer compound, e.g., DOTAP (Boehringer-Mannheim) or an equivalent such as lipofectin. The amount of the oligonucleotide used is about 500 nanograms in 3 micrograms of DOTAP per 100,000 cells. For electroporation, between 20 and 2000 nanograms of oligonucleotide per million cells to be electroporated is an appropriate range of dosages which can be increased to improve efficiency of genetic alteration upon review of the appropriate sequence according to the methods described herein. For biolistic delivery, microbeads are generally coated with resuspended oligonucleotides, which range of oligonucleotide to microbead concentration can be similarly adjusted to improve efficiency as determined using one of the assay methods described herein, starting with about 0.05 to 1 microgram of oligonucleotide to 25 microgram of 1.0 micrometer gold beads or similar microcarrier.  
       [0039] Another aspect of the invention is a kit comprising at least one oligonucleotide of the invention. The kit may comprise an additional reagent or article of manufacture. The additional reagent or article of manufacture may comprise a delivery mechanism, cell extract, a cell, or a plasmid, such as one of those disclosed in the Figures herein, for use in an assay of the invention. Alternatively, the invention includes a kit comprising an isogenic set of cells in which each cell in the kit comprises a different altered amino acid for a target protein encoded by a targeted altered gene within the cell produced according to the methods of the invention. 
     
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
     [0040]FIG. 1. Flow diagram for the generation of modified single-stranded oligonucleotides. The upper strands of chimeric oligonucleotides I and II are separated into pathways resulting in the generation of single-stranded oligonucleotides that contain (A) 2′-O-methyl RNA nucleotides or (B) phosphorothioate linkages. Fold changes in repair activity for correction of kan s  in the HUH7 cell-free extract are presented in parenthesis. HUH7 cells are described in Nakabayashi et al., Cancer Research 42: 3858-3863 (1982). Each single-stranded oligonucleotide is 25 bases in length and contains a G residue mismatched to the complementary sequence of the kan s  gene. The numbers 3, 6, 8, 10, 12 and 12.5 respectively indicate how many phosphorothioate linkages (S) or 2′-O-methyl RNA nucleotides (R) are at each end of the molecule. Hence oligo 12S/25G contains an all phosphorothioate backbone, displayed as a dotted line. Smooth lines indicate DNA residues, wavy lines indicate 2′-O-methyl RNA residues and the carat indicates the mismatched base site (G). FIG. 1(C) provides a schematic plasmid indicating the sequence of the kan chimeric double-stranded hairpin oligonucleotide (left; SEQ ID NO: 2673) and the sequence the tet chimeric double-stranded hairpin oligonucleotide used in other experiments (right; SEQ ID NO: 2674). FIG. 1(D) provides a flow chart of a kan experiment in which a chimeric double-stranded hairpin oligonucleotide (SEQ ID NO: 2673) is used. In FIG. 1(D), the Kan mutant sequence corresponds to SEQ ID NO: 2675 and SEQ ID NO: 2676; the Kan converted sequence corresponds to SEQ ID NO: 2677 and SEQ ID NO: 2678; the mutant sequence in the sequence trace corresponds to SEQ ID NO: 2679 and the converted sequences in the sequence trace correspond to SEQ ID NO: 2680.  
     [0041]FIG. 2. Genetic readout system for correction of a point mutation in plasmid pK s m4021. A mutant kanamycin gene harbored in plasmid pK s m4021 is the target for correction by oligonucleotides. The mutant G is converted to a C by the action of the oligo. Corrected plasmids confer resistance to kanamycin in  E.coli  (DH10B) after electroporation leading to the genetic readout and colony counts. The wild type sequence corresponds to SEQ ID NO: 2681.  
     [0042]FIG. 3: Target plasmid and sequence correction of a frameshift mutation by chimeric and single-stranded oligonucleotides. (A) Plasmid pT s Δ208 contains a single base deletion mutation at position 208 rendering it unable to confer tet resistance. The target sequence presented below indicates the insertion of a T directed by the oligonucleotides to re-establish the resistant phenotype. (B) DNA sequence confirming base insertion directed by Tet 3S/25G; the yellow highlight indicates the position of frameshift repair. The wild type sequence corresponds to SEQ ID NO: 2682, the mutant sequence corresponds to SEQ ID NO: 2683 and the converted sequence corresponds to SEQ ID NO: 2684. The control sequence in the sequence trace corresponds to SEQ ID NO: 2685 and the 3S/25A sequence in the sequence trace corresponds to SEQ ID NO: 2686.  
     [0043]FIG. 4. DNA sequences of representative kan r  colonies. Confirmation of sequence alteration directed by the indicated molecule is presented along with a table outlining codon distribution. Note that 10S/25G and 12S/25G elicit both mixed and unfaithful gene repair. The number of clones sequenced is listed in parentheses next to the designation for the single-stranded oligonucleotide. A plus (+) symbol indicates the codon identified while a figure after the (+) symbol indicates the number of colonies with a particular sequence. TAC/TAG indicates a mixed peak. Representative DNA sequences are presented below the table with yellow highlighting altered residues. The sequences in the sequence traces have been assigned numbers as follows: 3S/25G, 6S/25G and 8S/25G correspond to SEQ ID NO: 2687, 10S/25G corresponds to SEQ ID NO: 2688, 25S/25G on the lower left corresponds to SEQ ID NO: 2689 and 25S/25G on the lower right corresponds to SEQ ID NO: 2690.  
     [0044]FIG. 5. Gene correction in HeLa cells. Representative oligonucleotides of the invention are co-transfected with the pCMVneo( − )FIAsH plasmid (shown in FIG. 9) into HeLa cells. Ligand is diffused into cells after co-transfection of plasmid and oligonucleotides. Green fluorescence indicates gene correction of the mutation in the antibiotic resistance gene. Correction of the mutation results in the expression of a fusion protein that carries a marker ligand binding site and when the fusion protein binds the ligand, a green fluorescence is emitted. The ligand is produced by Aurora Biosciences and can readily diffuse into cells enabling a measurement of corrected protein function; the protein must bind the ligand directly to induce fluorescence. Hence cells bearing the corrected plasmid gene appear green while “uncorrected” cells remain colorless.  
     [0045]FIG. 6. Z-series imaging of corrected cells. Serial cross-sections of the HeLa cell represented in FIG. 5 are produced by Zeiss 510 LSM confocal microscope revealing that the fusion protein is contained within the cell.  
     [0046]FIG. 7. Hygromycin-eGFP target plasmids. (A) Plasmid pAURHYG(ins)GFP contains a single base insertion mutation between nucleotides 136 and 137, at codon 46, of the Hygromycin B coding sequence (cds) which is transcribed from the constitutive ADH1 promoter. The target sequence presented below indicates the deletion of an A and the substitution of a C for a T directed by the oligonucleotides to re-establish the resistant phenotype. In FIG. 7A, the sequence of the normal allele corresponds to SEQ ID NO: 2691, the sequence of the targe/existing mutation corresponds to SEQ ID NO: 2692 and the sequence of the desired alteration corresponds to SEQ ID NO: 2693. (B) Plasmid pAURHYG(rep)GFP contains a base substitution mutation introducing a G at nucleotide 137, at codon 46, of the Hygromycin B coding sequence (cds). The target sequence presented below the diagram indicates the amino acid conservative replacement of G with C, restoring gene function. In FIG. 7B, the sequence of the normal allele correspond to SEQ ID NO: 2691, the sequence of the targe/existing mutation corresponds to SEQ ID NO: 2694 and the sequence of the desired alteration corresponds to SEQ ID NO: 2693.  
     [0047]FIG. 8. Oligonucleotides for correction of hygromycin resistance gene. The sequence of the oligonucleotides used in experiments to assay correction of a hygromycin resistance gene are shown. DNA residues are shown in capital letters, RNA residues are shown in lowercase and nucleotides with a phosphorothioate backbone are capitalized and underlined. In FIG. 8, the sequence of HygE3T/25 corresponds to SEQ ID NO: 2695, the sequence of HygE3T/74 corresponds to SEQ ID NO: 2696, the sequence of HygE3T/74a corresponds to SEQ ID NO: 2697, the sequence of HygGG/Rev corresponds to SEQ ID NO: 2698 and the sequence of Kan70T corresponds to SEQ ID NO: 2699.  
     [0048]FIG. 9. pAURNeo(−)FIAsH plasmid. This figure describes the plasmid structure, target sequence, oligonucleotides, and the basis for detection of the gene alteration event by fluorescence. In FIG. 9, the sequence of the Neo/kan target mutant corresponds to SEQ ID NO: 2675 and SEQ ID NO: 2676, the converted sequence corresponds to SEQ ID NO: 2677 and SEQ ID NO: 2678 and the FIAsH peptide sequence corresponds to SEQ ID NO: 2700.  
     [0049]FIG. 10. pYESHyg(x)eGFP plasmid. This plasmid is a construct similar to the pAURHyg(x)eGFP construct shown in FIG. 7, except the promoter is the inducible GAL1 promoter. This promoter is inducible with galactose, leaky in the presence of raffinose, and repressed in the presence of dextrose.  
     [0050]FIG. 11. pBI-HygeGFP plasmid. This plasmid is a construct based on the plasmids pBI101, pBI 101.2, pBI101.3 or pBI 121 available from Clontech in which HygeGFP replaces the beta-glucuronidase gene of the Clontech plasmids. The different Clontech plasmids vary by a reading frame shift relative to the polylinker, or the presence of the Cauliflower mosaic virus promoter. 
    
    
     [0051] The following examples are provided by way of illustration only, and are not intended to limit the scope of the invention disclosed herein.  
     EXAMPLE 1  
     Assay Method for Base Alteration and Preferred Oligonucleotide Selection  
     [0052] In this example, single-stranded and double-hairpin oligonucleotides with chimeric backbones (see FIG. 1 for structures (A and B) and sequences (C and D) of assay oligonucleotides) are used to correct a point mutation in the kanamycin gene of pK s m4021 (FIG. 2) or the tetracycline gene of pT s Δ208 (FIG. 3). All kan oligonucleotides share the same 25 base sequence surrounding the target base identified for change, just as all tet oligonucleotides do. The sequence is given in FIG. 1C and FIG. 1D. Each plasmid contains a functional ampicillin gene. Kanamycin gene function is restored when a G at position 4021 is converted to a C (via a substitution mutation); tetracycline gene function is restored when a deletion at position 208 is replaced by a C (via frameshift mutation). A separate plasmid, pAURNeo(−)FIAsH (FIG. 9), bearing the kan s  gene is used in the cell culture experiments. This plasmid was constructed by inserting a synthetic expression cassette containing a neomycin phosphotransferasea (kanamycin resistance) gene and an extended reading frame that encodes a receptor for the FIAsH ligand into the pAUR123 shuttle vector (Panvera Corp., Madison, Wis.). The resulting construct replicates in  S. cerevisiae  at low copy number, confers resistance to aureobasidinA and constitutively expresses either the Neo+/FIAsH fusion product (after alteration) or the truncated Neo−/FIAsH product (before alteration) from the ADH1 promoter. By extending the reading frame of this gene to code for a unique peptide sequence capable of binding a small ligand to form a fluorescent complex, restoration of expression by correction of the stop codon can be detected in real time using confocal microscopy.  
     [0053] Additional constructs can be made to test additional gene alteration events or for specific use in different expression systems. For example, alternative comparable plant plasmids or integration vectors such as, e.g. those based on T-DNA, can be constructed for stable expression in plant cells according to the disclosures herein. Such constructs would use a plant specific promoter such as, e.g., cauliflower mosaic virus 35S promoter, to replace the promoters directing expression of the neo, hyg or aureobasidinA resistance gene disclosed herein, including for example, in FIGS. 7B, 9 and  10  herein. Moreover, the green fluorescent protein (GFP) sequence used herein may be modified to increase expression in plant cells such as Arabidopsis and the other plants disclosed herein as described in Haseloff et al., Proc. Natl.Acad. Sci. 94(6): 2122-7 (1997), Rouwendal et al. Plant Mol. Biol. 33(6): 989-99 (1997) and Hu et al. FEBS Lett. 369(2-3): 331-4 (1995). Codon usage for optimal expression of GFP in plants results from increasing the frequency of codons with a C or a G in the third position from 32 to about 60%. Specific constructs are disclosed and can be used as follows with such plant specific alterations.  
     [0054] We also construct three mammalian expression vectors, pHyg(rep)eGFP, pHyg(Δ)eGFP, pHyg(ins)eGFP, that contain a substitution mutation at nucleotide 137 of the hygromycin-B coding sequence. (rep) indicates a T1374→G replacement, (Δ) represents a deletion of the G137 and (ins) represents an A insertion between nucleotides 136 and 137. All point mutations create a nonsense termination codon at residue 46. We use pHYGeGFP plasmid (Invitrogen, CA) DNA as a template to introduce the mutations into the hygromycin-eGFP fusion gene by a two step site-directed mutagenesis PCR protocol. First, we generate overlapping 5′ and a 3′ amplicons surrounding the mutation site by PCR for each of the point mutation sites. A 215 bp 5′ amplicon for the (rep), (Δ) or (ins) was generated by polymerization from oligonucleotide primer HygEGFPf (5′-AATACGACTCACTATAGG-3′; SEQ ID NO: 2701) to primer Hygrepr (5′GACCTATCCACGCCCTCC-3′; SEQ ID NO: 2702), HygΔr (5′-GACTATCCACGCCCTCC-3′; SEQ ID NO: 2703), or Hyginsr (5′-GACATTATCCACGCCCTCC-3′; SEQ ID NO: 2704), respectively. We generate a 300 bp 3′ amplicon for the (rep), (Δ) or (ins) by polymerization from oligonucleotide primers Hygrepf (5′-CTGGGATAGGTCCTGCGG-3′; SEQ ID NO: 2705), HygΔf (5′-CGTGGATAGTCCTGCGG-3′; SEQ ID NO: 2706), Hyginsf (5′-CGTGGATAATGTCCTGCGG-3′; SEQ ID NO: 2707), respectively to primer HygEGFPr (5′-AAATCACGCCATGTAGTG-3′; SEQ ID NO: 2708). We mix 20 ng of each of the resultant 5′ and 3′ overlapping amplicon mutation sets and use the mixture as a template to amplify a 523 bp fragment of the Hygromycin gene spanning the KpnI and RsrII restriction endonuclease sites. We use the Expand PCR system (Roche) to generate all amplicons with 25 cycles of denaturing at 94° C. for 10 seconds, annealing at 55° C. for 20 seconds and elongation at 68° C. for 1 minute. We digest 10 μg of vector pHYGeGFP and 5 μg of the resulting fragments for each mutation with KpnI and RsrII (NEB) and gel purify the fragment for enzymatic ligation. We ligate each mutated insert into pHYGeGFP vector at 3:1 molar ratio using T4 DNA ligase (Roche). We screen clones by restriction digest, confirm the mutation by Sanger dideoxy chain termination sequencing and purify the plasmid using a Qiagen maxiprep kit.  
     [0055] Oligonucleotide synthesis and cells. Chimeric oligonucleotides and single-stranded oligonucleotides (including those with the indicated modifications) are synthesized using available phosphoramidites on controlled pore glass supports. After deprotection and detachment from the solid support, each oligonucleotide is gel-purified using, for example, procedures such as those described in Gamper et al.,  Biochem.  39, 5808-5816 (2000) and the concentrations determined spectrophotometrically (33 or 40 μg/ml per A 260  unit of single-stranded or hairpin oligomer). HUH7 cells are grown in DMEM, 10% FBS, 2 mM glutamine, 0.5% pen/strep. The  E.coli  strain, DH10B, is obtained from Life Technologies (Gaithersburg, Md.); DH10B cells contain a mutation in the RECA gene (recA).  
     [0056] Cell-free extracts. Although this portion of this example is directed to mammalian systems, similar extracts from plants can be prepared as disclosed elsewhere in this application and used as disclosed in this example. We prepare cell-free extracts from HUH7 cells or other mammalian cells, as follows. We employ this protocol with essentially any mammalian cell including, for example, H1299 cells (human epithelial carcinoma, non-small cell lung cancer), C127I (immortal murine mammary epithelial cells), MEF (mouse embryonic fibroblasts), HEC-1-A (human uterine carcinoma), HCT15 (human colon cancer), HCT116 (human colon carcinoma), LoVo (human colon adenocarcinoma), and HeLa (human cervical carcinoma). We harvest approximately 2×10 8  cells. We then wash the cells immediately in cold hypotonic buffer (20 mM HEPES, pH7.5; 5 mM KCl; 1.5 mM MgCl 2 ; 1 mM DTT) with 250 mM sucrose. We then resuspend the cells in cold hypotonic buffer without sucrose and after 15 minutes we lyse the cells with 25 strokes of a Dounce homogenizer using a tight fitting pestle. We incubate the lysed cells for 60 minutes on ice and centrifuge the sample for 15 minutes at 12000×g. The cytoplasmic fraction is enriched with nuclear proteins due to the extended co-incubation of the fractions following cell breakage. We then immediately aliquote and freeze the supernatant at −80° C. We determine the protein concentration in the extract by the Bradford assay.  
     [0057] We also perform these experiments with cell-free extracts obtained from fungal cells, including, for example,  S. cerevisiae  (yeast),  Ustilago maydis,  and  Candida albicans.  For example, we grow yeast cells into log phase in 2L YPD medium for 3 days at 30° C. We then centrifuge the cultures at 5000×g, resuspend the pellets in a 10% sucrose, 50 mM Tris, 1 mM EDTA lysis solution and freeze them on dry ice. After thawing, we add KCl, spermidine and lyticase to final concentrations of 0.25 mM, 5 mM and 0.1 mg/ml, respectively. We incubate the suspension on ice for 60 minutes, add PMSF and Triton X100 to final concentrations of 0.1 mM and 0.1% and continue to incubate on ice for 20 minutes. We centrifuge the lysate at 3000×g for 10 minutes to remove larger debris. We then remove the supernatant and clarify it by centrifuging at 30000×g for 15 minutes. We then add glycerol to the clarified extract to a concentration of 10% (v/v) and freeze aliquots at −80° C. We determine the protein concentration of the extract by the Bradford assay.  
     [0058] Reaction mixtures of 50 μl are used, consisting of 10-30 μg protein of cell-free extract, which can be optionally substituted with purified proteins or enriched fractions, about 1.5 μg chimeric double-hairpin oligonucleotide or 0.55 μg single-stranded molecule (3S/25G or 6S/25G, see FIG. 1), and 1 μg of plasmid DNA (see FIGS. 2 and 3) in a reaction buffer of 20 mM Tris, pH 7.4, 15 mM MgCl 2 , 0.4 mM DTT, and 1.0 mM ATP. Reactions are initiated with extract and incubated at 30° C. for 45 min. The reaction is stopped by placing the tubes on ice and then immediately deproteinized by two phenol/chloroform (1:1) extractions. Samples are then ethanol precipitated. The nucleic acid is pelleted at 15,000 r.p.m. at 4° C. for 30 min., is washed with 70% ethanol, resuspended in 50 μl H 2 O, and is stored at −20° C. 5 μl of plasmid from the resuspension (˜100 ng) was transfected in 20 μl of DH10B cells by electroporation (400 V, 300 μF, 4 kΩ) in a Cell-Porator apparatus (Life Technologies). After electroporation, cells are transferred to a 14 ml Falcon snap-cap tube with 2 ml SOC and shaken at 37° C. for 1 h. Enhancement of final kan colony counts is achieved by then adding 3 ml SOC with 10 μg/ml kanamycin and the cell suspension is shaken for a further 2 h at 37° C. Cells are then spun down at 3750×g and the pellet is resuspended in 500 μl SOC. 200 μl is added undiluted to each of two kanamycin (50 μg/ml) agar plates and 200 μl of a 10 5  dilution is added to an ampicillin (100 μg/ml) plate. After overnight 37° C. incubation, bacterial colonies are counted using an Accucount 1000 (Biologics). Gene conversion effectiveness is measured as the ratio of the average of the kan colonies on both plates per amp colonies multiplied by 10 −5  to correct for the amp dilution.  
     [0059] The following procedure can also be used. 5 μl of resuspended reaction mixtures (total volume 50 μl) are used to transform 20 μl aliquots of electro-competent DH10B bacteria using a Cell-Porator apparatus (Life Technologies). The mixtures are allowed to recover in 1 ml SOC at 37° C. for 1 hour at which time 50 μg/ml kanamycin or 12 μg/ml tetracycline is added for an additional 3 hours. Prior to plating, the bacteria are pelleted and resuspended in 200 μl of SOC. 100 μl aliquots are plated onto kan or tet agar plates and 100 μl of a 10 31 4  dilution of the cultures are concurrently plated on agar plates containing 100 μg/ml of ampicillin. Plating is performed in triplicate using sterile Pyrex beads. Colony counts are determined by an Accu-count 1000 plate reader (Biologics). Each plate contains 200-500 ampicillin resistant colonies or 0-500 tetracycline or kanamycin resistant colonies. Resistant colonies are selected for plasmid extraction and DNA sequencing using an ABI Prism kit on an ABI 310 capillary sequencer (PE Biosystems).  
     [0060] Chimeric single-stranded oligonucleotides. In FIG. 1 the upper strands of chimeric oligonucleotides I and II are separated into pathways resulting in the generation of single-stranded oligo-nucleotides that contain (FIG. 1A) 2′-O-methyl RNA nucleotides or (FIG. 1B) phosphorothioate linkages. Fold changes in repair activity for correction of kan s  in the HUH7 cell-free extract are presented in parenthesis. Each single-stranded oligonucleotide is 25 bases in length and contains a G residue mismatched to the complementary sequence of the kan s  gene.  
     [0061] Molecules bearing 3, 6, 8, 10 and 12 phosphorothioate linkages in the terminal regions at each end of a backbone with a total of 24 linkages (25 bases) are tested in the kan s  system. Alternatively, molecules bearing 2, 4, 5, 7, 9 and 11 in the terminal regions at each end are tested. The results of one such experiment, presented in Table 1 and FIG. 1B, illustrate an enhancement of correction activity directed by some of these modified structures. In this illustrative example, the most efficient molecules contained 3 or 6 phosphorothioate linkages at each end of the 25-mer; the activities are approximately equal (molecules IX and X with results of 3.09 and 3.7 respectively). A reduction in alteration activity may be observed as the number of modified linkages in the molecule is further increased. Interestingly, a single-strand molecule containing 24 phosphorothioate linkages is minimally active suggesting that this backbone modification when used throughout the molecule supports only a low level of targeted gene repair or alteration. Such a non-altering, completely modified molecule can provide a baseline control for determining efficiency of correction for a specific oligonucleotide molecule of known sequence in defining the optimum oligonucleotide for a particular alteration event.  
     [0062] The efficiency of gene repair directed by phosphorothioate-modified, single-stranded molecules, in a length dependent fashion, led us to examine the length of the RNA modification used in the original chimera as it relates to correction. Construct III represents the “RNA-containing” strand of chimera I and, as shown in Table 1 and FIG. 2A, it promotes inefficient gene repair. But, as shown in the same figure, reducing the RNA residues on each end from 10 to 3 increases the frequency of repair. At equal levels of modification, however, 25-mers with 2′-O-methyl ribonucleotides were less effective gene repair agents than the same oligomers with phosphorothioate linkages. These results reinforce the fact that an RNA containing oligonucleotide is not as effective in promoting gene repair or alteration as a modified DNA oligonucleotide.  
     [0063] Repair of the kanamycin mutation requires a G→C exchange. To confirm that the specific desired correction alteration was obtained, colonies selected at random from multiple experiments are processed and the isolated plasmid DNA is sequenced. As seen in FIG. 4, colonies generated through the action of the single-stranded molecules 3S/25G (IX), 6S/25G (X) and 8S/25G (XI) respectively contained plasmid molecules harboring the targeted base correction. While a few colonies appeared on plates derived from reaction mixtures containing 25-mers with 10 or 12 thioate linkages on both ends, the sequences of the plasmid molecules from these colonies contain nonspecific base changes. In these illustrative examples, the second base of the codon is changed (see FIG. 3). These results show that modified single-strands can direct gene repair, but that efficiency and specificity are reduced when the 25-mers contain 10 or more phosphorothioate linkages at each end.  
     [0064] In FIG. 1, the numbers 3, 6, 8, 10, 12 and 12.5 respectively indicate how many phosphorothioate linkages (S) or 2′-O-methyl RNA nucleotides (R) are at each end of the examplified molecule although other molecules with 2, 4, 5, 7, 9 and 11 modifications at each end can also be tested. Hence oligo 12S/25G represents a 25-mer oligonucleotide which contains 12 phosphorothioate linkages on each side of the central G target mismatch base producing a fully phosphorothioate linked backbone, displayed as a dotted line. The dots are merely representative of a linkage in the figure and do not depict the actual number of linkages of the oligonucleotide. Smooth lines indicate DNA residues, wavy lines indicate 2′-O-methyl RNA residues and the carat indicates the mismatched base site (G).  
     [0065] Correction of a mutant kanamycin gene in cultured mammalian cells. Although this portion of this example is directed to cultured mammalian cells, comparable methods may be used using cultured plant cells or protoplasts of those cells from the plant species disclosed herein. The experiments are performed using different eukaryotic cells including plant and mammalian cells, including, for example, 293 cells (transformed human primary kidney cells), HeLa cells (human cervical carcinoma), and H1299 (human epithelial carcinoma, non-small cell lung cancer). HeLa cells are grown at 37° C. and 5% CO 2  in a humidified incubator to a density of 2×10 5  cells/ml in an 8 chamber slide (Lab-Tek). After replacing the regular DMEM with Optimem, the cells are co-transfected with 10 μg of plasmid pAURNeo(−) FIAsH and 5 μg of modified single-stranded oligonucleotide (3S/25G) that is previously complexed with 10 μg lipofectamine, according to the manufacturer&#39;s directions (Life Technologies). The cells are treated with the liposome-DNA-oligo mix for 6 hrs at 37° C. Treated cells are washed with PBS and fresh DMEM is added. After a 16-18 hr recovery period, the culture is assayed for gene repair. The same oligonucleotide used in the cell-free extract experiments is used to target transfected plasmid bearing the kan s  gene. Correction of the point mutation in this gene eliminates a stop codon and restores full expression. This expression can be detected by adding a small non-fluorescent ligand that bound to a C-C-R-E-C-C sequence (SEQ ID NO: 2717) in the genetically modified carboxy terminus of the kan protein, to produce a highly fluorescent complex (FIAsH system, Aurora Biosciences Corporation). Following a 60 min incubation at room temperature with the ligand (FIAsH-EDT2), cells expressing full length kan product acquire an intense green fluorescence detectable by fluorescence microscopy using a fluorescein filter set. Similar experiments are performed using the HygeGFP target as described in Example 2 with a variety of mammalian cells, including, for example, COS-1 and COS-7 cells (African green monkey), and CHO-K1 cells (Chinese hamster ovary). The experiments are also performed with PG12 cells (rat pheochromocytoma) and ES cells (human embryonic stem cells).  
     [0066] Summary of experimental results. Tables 1, 2 and 3 respectively provide data on the efficiency of gene repair directed by single-stranded oligonucleotides. Table 1 presents data using a cell-free extract from human liver cells (HUH7) to catalyze repair of the point mutation in plasmid pkan s m4021 (see FIG. 1). Table 2 illustrates that the oligomers are not dependent on MSH2 or MSH3 for optimal gene repair activity. Table 3 illustrates data from the repair of a frameshift mutation (FIG. 3) in the tet gene contained in plasmid pTetΔ208. Table 4 illustrates data from repair of the pkan s m4021 point mutation catalyzed by plant cell extracts prepared from canola and musa (banana). Colony numbers are presented as kan r  or tet r  and fold increases (single strand versus double hairpin) are presented for kan r  in Table 1.  
     [0067]FIG. 5A is a confocal picture of HeLa cells expressing the corrected fusion protein from an episomal target. Gene repair is accomplished by the action of a modified single-stranded oligonucleotide containing 3 phosphorothioate linkages at each end (3S/25G). FIG. 5B represents a “Z-series” of HeLa cells bearing the corrected fusion gene. This series sections the cells from bottom to top and illustrates that the fluorescent signal is “inside the cells”.  
     [0068] Results. In summary, we have designed a novel class of single-stranded oligonucleotides with backbone modifications at the termini and demonstrate gene repair/conversion activity in mammalian and plant cell-free extracts. We confirm that the all DNA strand of the RNA-DNA double-stranded double hairpin chimera is the active component in the process of gene repair. In some cases, the relative frequency of repair by the novel oligonucleotides of the invention is elevated approximately 3-4-fold in certain embodiments when compared to frequencies directed by chimeric RNA-DNA double hairpin oligonucleotides.  
     [0069] This strategy centers around the use of extracts from various sources to correct a mutation in a plasmid using a modified single-stranded or a chimeric RNA-DNA double hairpin oligonucleotide. A mutation is placed inside the coding region of a gene conferring antibiotic resistance in bacteria, here kanamycin or tetracycline. The appearance of resistance is measured by genetic readout in  E.coli  grown in the presence of the specified antibiotic. The importance of this system is that both phenotypic alteration and genetic inheritance can be measured. Plasmid pK s m4021 contains a mutation (T→G) at residue 4021 rendering it unable to confer antibiotic resistance in  E.coli.  This point mutation is targeted for repair by oligonucleotides designed to restore kanamycin resistance. To avoid concerns of plasmid contamination skewing the colony counts, the directed correction is from G→C rather than G→T (wild-type). After isolation, the plasmid is electroporated into the DH10B strain of  E.coli,  which contains inactive RecA protein. The number of kanamycin colonies is counted and normalized by ascertaining the number of ampicillin colonies, a process that controls for the influence of electroporation. The number of colonies generated from three to five independent reactions was averaged and is presented for each experiment. A fold increase number is recorded to aid in comparison.  
     [0070] The original RNA-DNA double hairpin chimera design, e.g., as disclosed in U.S. Pat. No. 5,565,350, consists of two hybridized regions of a single-stranded oligonucleotide folded into a double hairpin configuration. The double-stranded targeting region is made up of a 5 base pair DNA/DNA segment bracketed by 10 base pair RNA/DNA segments. The central base pair is mismatched to the corresponding base pair in the target gene. When a molecule of this design is used to correct the kan s  mutation, gene repair is observed (I in FIG. 1A). Chimera II (FIG. 1B) differs partly from chimera I in that only the DNA strand of the double hairpin is mismatched to the target sequence. When this chimera was used to correct the kan s  mutation, it was twice as active. In the same study, repair function could be further increased by making the targeting region of the chimera a continuous RNA/DNA hybrid.  
     [0071] Frame shift mutations are repaired. By using plasmid pT s Δ208, described in FIG. 1(C) and FIG. 3, the capacity of the modified single-stranded molecules that showed activity in correcting a point mutation, can be tested for repair of a frameshift. To determine efficiency of correction of the mutation, a chimeric oligonucleotide (Tet I), which is designed to insert a T residue at position 208, is used. A modified single-stranded oligonucleotide (Tet IX) directs the insertion of a T residue at this same site. FIG. 3 illustrates the plasmid and target bases designated for change in the experiments. When all reaction components are present (extract, plasmid, oligomer), tetracycline resistant colonies appear. The colony count increases with the amount of oligonucleotide used up to a point beyond which the count falls off (Table 3). No colonies above background are observed in the absence of either extract or oligonucleotide, nor when a modified single-stranded molecule bearing perfect complementarity is used. FIG. 3 represents the sequence surrounding the target site and shows that a T residue is inserted at the correct site. We have isolated plasmids from fifteen colonies obtained in three independent experiments and each analyzed sequence revealed the same precise nucleotide insertion. These data suggest that the single-stranded molecules used initially for point mutation correction can also repair nucleotide deletions.  
     [0072] Comparison of phosphorothioate oligonucleotides to 2′-O-methyl substituted oligonucleotides. From a comparison of molecules VII and XI, it is apparent that gene repair is more subject to inhibition by RNA residues than by phosphorothioate linkages. Thus, even though both of these oligonucleotides contain an equal number of modifications to impart nuclease resistance, XI (with 16 phosphorothioate linkages) has good gene repair activity while VII (with 16 2′-O-methyl RNA residues) is inactive. Hence, the original chimeric double hairpin oligonucleotide enabled correction directed, in large part, by the strand containing a large region of contiguous DNA residues.  
     [0073] Oligonucleotides can target multiple nucleotide alterations within the same template. The ability of individual single-stranded oligonucleotides to correct multiple mutations in a single target template is tested using the plasmid pK s m4021 and the following single-stranded oligonucleotides modified with 3 phosphorothioate linkages at each end (indicated as underlined nucleotides): Oligo1 is a 25-mer with the sequence  TTC GATAAGCCTATGCTGACCC GTG  (SEQ ID NO: 2709) corrects the original mutation present in the kanamycin resistance gene of pK s m4021 as well as directing another alteration 2 basepairs away in the target sequence (both indicated in boldface); Oligo2 is a 70-mer with the 5′-end sequence  TTC GGCTACGACTGGGCACAACAGACAATTGGC (SEQ ID NO: 2710) with the remaining nucleotides being completely complementary to the kanamycin resistance gene and also ending in 3 phosphorothioate linkages at the 3′ end. Oigo2 directs correction of the mutation in pK s m4021 as well as directing another alteration 21 basepairs away in the target sequence (both indicated in boldface).  
     [0074] We also use additional oligonucleotides to assay the ability of individual oligonucleotides to correct multiple mutations in the pK s M4021 plasmid. These include, for example, a second 25-mer that alters two nucleotides that are three nucleotides apart with the sequence 5′-TTGTGCCCAGTC G TA T CCGAATAGC-3′ (SEQ ID NO: 2711); a 70-mer that alters two nucleotides that are 21 nucleotides apart with the sequence 5′-CATCAGAGCAGCC A ATTGTCTGTTGTGCCCAGTC G TAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGA-3′ (SEQ ID NO: 2712); and another 70-mer that alters two nucleotides that are 21 nucleotides apart with the sequence 5′-GCTGACAGCCGGAACACGGCGGCATCAGAGCAGCC A ATTGTCTGTTGTGCCCAGTC G TAGCCGMTAGCCT-3′ (SEQ ID NO: 2713). The nucleotides in the oligonucleotides that direct alteration of the target sequence are underlined and in boldface. These oligonucleotides are modified in the same way as the other oligonucleotides of the invention.  
     [0075] We assay correction of the original mutation in pK s m4021 by monitoring kanamycin resistance (the second alterations which are directed by Oligo2 and Oligo3 are silent with respect to the kanamycin resistance phenotype). In addition, in experiments with Oligo2, we also monitor cleavage of the resulting plasmids using the restriction enzyme Tsp5091 which cuts at a specific site present only when the second alteration has occurred (at ATT in Oligo2). We then sequence these clones to determine whether the additional, silent alteration has also been introduced. The results of an analysis are presented below:  
                                                   Oligo 1 (25-mer)   Oligo 2 (70-mer)                                                Clones with both sites changed   9   7       Clones with a single site changed   0   2       Clones that were not changed   4   1                  
 
     [0076] Nuclease sensitivity of unmodified DNA oligonucleotide. Electrophoretic analysis of nucleic acid recovered from the cell-free extract reactions conducted here confirm that the unmodified single-stranded 25-mer did not survive incubation whereas greater than 90% of the terminally modified oligos did survive (as judged by photo-image analyses of agarose gels).  
     [0077] Plant extracts direct repair. The modified single-stranded constructs can be tested in plant cell extracts. We have observed gene alteration using extracts from multiple plant sources, including, for example, Arabidopsis, tobacco, banana, maize, soybean, canola, wheat, spinach as well as spinach chloroplast extract or extracts made from other plant cells disclosed herein. We prepare the extracts by grinding plant tissue or cultured cells under liquid nitrogen with a mortar and pestle. We extract 3 ml of the ground plant tissue with 1.5 ml of extraction buffer (20 mM HEPES, pH7.5; 5 mM KCl; 1.5 mM MgCl 2 ; 10 mM DTT; and 10% [v/v] glycerol). Some plant cell-free extracts also include about 1% (w/v) PVP. We then homogenize the samples with 15 strokes of a Dounce homogenizer. Following homogenization, we incubate the samples on ice for 1 hour and centrifuge at 3000×g for 5 minutes to remove plant cell debris. We then determine the protein concentration in the supernatants (extracts) by Bradford assay. We dispense 100 μg (protein) aliquots of the extracts which we freeze in a dry ice-ethanol bath and store at −80° C.  
     [0078] We describe experiments using two sources here: a dicot (canola) and a monocot (banana,  Musa acuminata  cv. Rasthali). Each vector directs gene repair of the kanamycin mutation (Table 4); however, the level of correction is elevated 2-3 fold relative to the frequency observed with the chimeric oligonucleotide. These results are similar to those observed in the mammalian system wherein a significant improvement in gene repair occurred when modified single-stranded molecules were used.  
     [0079] Tables are attached hereto.  
               TABLE I                          Gene repair activity is directed by single-stranded oligonucleotides.                                 Oligonucleotide   Plasmid   Extract (ug)   kan r  colonies   Fold increase                                         I   pK S m4021   10   300           I   ↓   20   418    1.0 ×       II   ↓   10   537       II   ↓   20   748    1.78 ×       III   ↓   10   3       III   ↓   20   5    0.01 ×       IV   ↓   10   112       IV   ↓   20   96    0.22 ×       V   ↓   10   217       V   ↓   20   342    0.81 ×       VI   ↓   10   6       VI   ↓   20   39   0.093 ×       VII   ↓   10   0       VII   ↓   20   0      0 ×       VIII   ↓   10   3       VIII   ↓   20   5    0.01 ×       IX   ↓   10   936       IX   ↓   20   1295    3.09 ×       X   ↓   10   1140       X   ↓   20   1588    3.7 ×       XI   ↓   10   480       XI   ↓   20   681    1.6 ×       XII   ↓   10   18       XII   ↓   20   25   0.059 ×       XIII   ↓   10   0       XIII   ↓   20   4   0.009 ×       —   ↓   20   0       I   ↓   —   0                  
 
     [0080] Plasmid pK S m4021 (1 μg), the indicated oligonucleotide (1.5 μg chimeric oligonucleotide or 0.55 μg single-stranded oligonucleotide; molar ratio of oligo to plasmid of 360 to 1) and either 10 or 20 μg of HUH7 cell-free extract were incubated 45 min at 37° C. Isolated plasmid DNA was electroporated into  E. coli  (strain DH10B) and the number of kan r  colonies counted. The data represent the number of kanamycin resistant colonies per 10 6  ampicillin resistant colonies generated from the same reaction and is the average of three experiments (standard deviation usually less than +/−15%). Fold increase is defined relative to 418 kan r  colonies (second reaction) and in all reactions was calculated using the 20 μg sample.  
               TABLE II                          Modified single-stranded oligomers are not dependent on MSH2       or MSH3 for optimal gene repair activity.                                         A.   Oligonucleotide   Plasmid   Extract   kan r  colonies                                                         IX (3S/25G)   ↓   HUH7   637               X (6S/25G)   ↓   HUH7   836               IX   ↓   MEF2 −/−     781               X   ↓   MEF2 −/−     676               IX   ↓   MEF3 −/−     582               X   ↓   MEF3 −/−     530               IX   ↓   MEF +/+     332               X   ↓   MEF +/+     497               —   ↓   MEF2 −/−     10               —   ↓   MEF3 −/−     5               —   ↓   MEF +/+     14                      
 
     [0081] Chimeric oligonucleotide (1.5 μg) or modified single-stranded oligonucleotide (0.55 μg) was incubated with 1 μg of plasmid pK S m4021 and 20 μg of the indicated extracts. MEF represents mouse embryonic fibroblasts with either MSH2 (2 −/− ) or MSH3 (3 −/− ) deleted. MEF +/+  indicates wild-type mouse embryonic fibroblasts. The other reaction components were then added and processed through the bacterial readout system. The data represent the number of kanamycin resistant colonies per 10 6  ampicillin resistant colonies.  
               TABLE III                          Frameshift mutation repair is directed by       single-stranded oligonucleotides                             Oligonucleotide   Plasmid   Extract   tet r  colonies                                         Tet IX (3S/25A; 0.5 μg)   pT S Δ208 (1 μg)       —   0       —   ↓   20 μg       0       Tet IX (0.5 μg)   ↓   ↓       48       Tet IX (1.5 μg)   ↓   ↓       130       Tet IX (2.0 μg)   ↓   ↓       68       Tet I (chimera; 1.5 μg)   ↓   ↓       48                  
 
     [0082] Each reaction mixture contained the indicated amounts of plasmid and oligonucleotide. The extract used for these experiments came from HUH7 cells. The data represent the number of tetracycline resistant colonies per 10 6  ampicillin resistant colonies generated from the same reaction and is the average of 3 independent experiments. Tet I is a chimeric oligonucleotide and Tet IX is a modified single-stranded oligonucleotide that are designed to insert a T residue at position 208 of pT s Δ208. The oligonucleotides are equivalent to structures I and IX in FIG. 2.  
               TABLE IV                          Plant cell-free extracts support gene repair by       single-stranded oligonucleotides                             Oligonucleotide   Plasmid   Extract   kan r  colonies                                         II (chimera)   pK S m402l   30 μg   Canola   337       IX (3S/25G)   ↓       Canola   763       X (6S/25G)   ↓       Canola   882       II   ↓       Musa   203       IX   ↓       Musa   343       X   ↓       Musa   746       —   ↓       Canola   0       —   ↓       Musa   0       IX   ↓   —   Canola   0       X   ↓   —   Musa   0                  
 
     [0083] Canola or Musa cell-free extracts were tested for gene repair activity on the kanamycin-sensitive gene as previously described in (18). Chimeric oligonucleotide II (1.5 μg) and modified single-stranded oligonucleotides IX and X (0.55 μg) were used to correct pK S m4021. Total number of kan r  colonies are present per 10 7  ampicillin resistant colonies and represent an average of four independent experiments.  
               TABLE V                          Gene repair activity in cell-free extracts prepared from yeast       ( Saccharomyces cerevisiae )                                 Cell-type   Plasmid   Chimeric Oligo   SS Oligo   kan r /amp r  × 10 6                                           Wild type   pKan s m4021   1 μg       0.36       Wild type   ↓       1 μg   0.81       ΔRAD52   ↓   1 μg       10.72       ΔRAD52   ↓       1 μg   17.41       ΔPMS1   ↓   1 μg       2.02       ΔPMS1   ↓       1 μg   3.23                          
 
     EXAMPLE 2  
     Yeast Cell Targeting Assay Method for Base Alteration and Preferred Oligonucleotide Selection  
     [0084] In this example, single-stranded oligonucleotides with modified backbones and double-hairpin oligonucleotides with chimeric, RNA-DNA backbones are used to measure gene repair using two episomal targets with a fusion between a hygromycin resistance gene and eGFP as a target for gene repair. These plasmids are pAURHYG(rep)GFP, which contains a point mutation in the hygromycin resistance gene (FIG. 7), pAURHYG(ins)GFP, which contains a single-base insertion in the hygromycin resistance gene (FIG. 7) and pAURHYG(Δ)GFP which has a single base deletion. We also use the plasmid containing a wild-type copy of the hygromycin-eGFP fusion gene, designated pAURHYG(wt)GFP, as a control. These plasmids also contain an aureobasidinA resistance gene. In pAURHYG(rep)GFP, hygromycin resistance gene function and green fluorescence from the eGFP protein are restored when a G at position 137, at codon 46 of the hygromycin B coding sequence, is converted to a C thus removing a premature stop codon in the hygromycin resistance gene coding region. In pAURHYG(ins)GFP, hygromycin resistance gene function and green fluorescence from the eGFP protein are restored when an A inserted between nucleotide positions 136 and 137, at codon 46 of the hygromycin B coding sequence, is deleted and a C is substituted for the T at position 137, thus correcting a frameshift mutation and restoring the reading frame of the hygromycin-eGFP fusion gene.  
     [0085] We synthesize the set of three yeast expression constructs pAURHYG(rep)eGFP, pAURHYG(Δ)eGFP, pAURHYG(ins)eGFP, that contain a point mutation at nucleotide 137 of the hygromycin-B coding sequence as follows. (rep) indicates a T137→G replacement, (Δ) represents a deletion of the G137 and (ins) represents an A insertion between nucleotides 136 and 137. We construct this set of plasmids by excising the respective expression cassettes by restriction digest from pHyg(x)EGFP and ligation into pAUR123 (Panvera, Calif.). We digest 10 μg pAUR123 vector DNA, as well as, 10 μg of each pHyg(x)EGFP construct with KpnI and SaII (NEB). We gel purify each of the DNA fragments and prepare them for enzymatic ligation. We ligate each mutated insert into pHygEGFP vector at 3:1 molar ratio using T4 DNA ligase (Roche). We screen clones by restriction digest, confirm by Sanger dideoxy chain termination sequencing and purify using a Qiagen maxiprep kit.  
     [0086] We use this system to assay the ability of five oligonucleotides (shown in FIG. 8) to support correction under a variety of conditions. The oligonucleotides which direct correction of the mutation in pAURHYG(rep)GFP can also direct correction of the mutation in pAURHYG(ins)GFP. Three of the four oligonucleotides (HygE3T/25, HygE3T/74 and HygGG/Rev) share the same 25-base sequence surrounding the base targeted for alteration. HygGG/Rev is an RNA-DNA chimeric double hairpin oligonucleotide of the type described in the prior art. One of these oligonucleotides, HygE3T/74, is a 74-base oligonucleotide with the 25-base sequence centrally positioned. The fourth oligonucleotide, designated HygE3T/74α, is the reverse complement of HygE3T/74. The fifth oligonucleotide, designated Kan70T, is a non-specific, control oligonucleotide which is not complementary to the target sequence. Alternatively, an oligonucleotide of identical sequence but lacking a mismatch to the target or a completely thioate modified oligonucleotide or a completely 2-O-methylated modified oligonucleotide may be used as a control. Alternatively, oligonucleotides containing one, two, three, four, five, six, eight, ten or more LNA modifications on at least one of the two termini (and preferrably the 3′ terminus) may be used in different embodiments.  
     [0087] Oligonucleotide synthesis and cells. We synthesized and purified the chimeric, double-hairpin oligonucleotides and single-stranded oligonucleotides (including those with the indicated modifications) as described in Example 1. Plasmids used for assay were maintained stably in yeast ( Saccharomyces cerevisiae ) strain LSY678 MAT α at low copy number under aureobasidin selection. Plasmids and oligonucleotides are introduced into yeast cells by electroporation as follows: to prepare electrocompetent yeast cells, we inoculate 10 ml of YPD media from a single colony and grow the cultures overnight with shaking at 300 rpm at 30° C. We then add 30 ml of fresh YPD media to the overnight cultures and continue shaking at 30° C. until the OD 600  was between 0.5 and 1.0 (3-5 hours). We then wash the cells by centrifuging at 4° C. at 3000 rpm for 5 minutes and twice resuspending the cells in 25 ml ice-cold distilled water. We then centrifuge at 4° C. at 3000 rpm for 5 minutes and resuspend in 1 ml ice-cold 1M sorbitol and then finally centrifuge the cells at 4° C. at 5000 rpm for 5 minutes and resuspend the cells in 120 μl 1M sorbitol. To transform electrocompetent cells with plasmids or oligonucleotides, we mix 40 μl of cells with 5 μg of nucleic acid, unless otherwise stated, and incubate on ice for 5 minutes. We then transfer the mixture to a 0.2 cm electroporation cuvette and electroporate with a BIO-RAD Gene Pulser apparatus at 1.5 kV, 25 μF, 200 Ω for one five-second pulse. We then immediately resuspend the cells in 1 ml YPD supplemented with 1M sorbitol and incubate the cultures at 30° C. with shaking at 300 rpm for 6 hours. We then spread 200 μl of this culture on selective plates containing 300 μg/ml hygromycin and spread 200 μl of a 10 5  dilution of this culture on selective plates containing 500 ng/ml aureobasidinA and/or and incubate at 30° C. for 3 days to allow individual yeast colonies to grow. We then count the colonies on the plates and calculate the gene conversion efficiency by determining the number of hygromycin resistance colonies per 10 5  aureobasidinA resistant colonies.  
     [0088] Frameshift mutations are repaired in yeast cells. We test the ability of the oligonucleotides shown in FIG. 8 to correct a frameshift mutation in vivo using LSY678 yeast cells containing the plasmid pAURHYG(ins)GFP. These experiments, presented in Table 6, indicate that these oligonucleotides can support gene correction in yeast cells. These data reinforce the results described in Example 1 indicating that oligonucleotides comprising phosphorothioate linkages facilitate gene correction much more efficiently than control duplex, chimeric RNA-DNA oligonucleotides. This gene correction activity is also specific as transformation of cells with the control oligonucleotide Kan70T produced no hygromycin resistant colonies above background and thus Kan70T did not support gene correction in this system. In addition, we observe that the 74-base oligonucleotide (HygE3T/74) corrects the mutation in pAURHYG(ins)GFP approximately five-fold more efficiently than the 25-base oligonucleotide (HygE3T/25). We also perform control experiments with LSY678 yeast cells containing the plasmid pAURHYG(wt)GFP. With this strain we observed that even without added oligonucleotides, there are too many hygromycin resistant colonies to count.  
     [0089] We also use additional oligonucleotides to assay the ability of individual oligonucleotides to correct multiple mutations in the pAURHYG(x)eGFP plasmid. These include, for example, one that alters two basepairs that are 3 nucleotides apart is a 74-mer with the sequence 5′-CTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGG G TA C GTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTAC-3′ (SEQ ID NO: 2714); a 74-mer that alters two basepairs that are 15 nucleotides apart with the sequence 5′-CTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATA C GTCCTGCGGGTAAA C AGCTGCGCCGATGGTTTCTAC-3′ (SEQ ID NO: 2715); and a 74-mer that alters two basepairs that are 27 nucleotides apart with the sequence 5′-CTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATA C GTCCTGCGGGTAAATAGCTGCGCCGA C GGTTTCTAC (SEQ ID NO: 2716). The nucleotides in these oligonucleotides that direct alteration of the target sequence are underlined and in boldface. These oligonucleotides are modified in the same ways as the other oligonucleotides of the invention.  
     [0090] Oligonucleotides targeting the sense strand direct gene correction more efficiently. We compare the ability of single-stranded oligonucleotides to target each of the two strands of the target sequence of both pAURHYG(ins)GFP and pAURHYG(rep)GFP. These experiments, presented in Tables 7 and 8, indicate that an oligonucleotide, HygE3T/74α, with sequence complementary to the sense strand (i.e. the strand of the target sequence that is identical to the mRNA) of the target sequence facilitates gene correction approximately ten-fold more efficiently than an oligonucleotide, HygE3T/74, with sequence complementary to the non-transcribed strand which serves as the template for the synthesis of RNA. As indicated in Table 7, this effect was observed over a range of oligonucleotide concentrations from 0-3.6 μg, although we did observe some variability in the difference between the two oligonucleotides (indicated in Table 7 as a fold difference between HygE3T/74α and HygE3T/74). Furthermore, as shown in Table 8, we observe increased efficiency of correction by HygE3T/74α relative to HygE3T/74 regardless of whether the oligonucleotides were used to correct the base substitution mutation in pAURHYG(rep)GFP or the insertion mutation in pAURHYG(ins)GFP. The data presented in Table 8 further indicate that the single-stranded oligonucleotides correct a base substitution mutation more efficiently than an insertion mutation. However, this last effect was much less pronounced and the oligonucleotides of the invention are clearly able efficiently to correct both types of mutations in yeast cells. In addition, the role of transcription is investigated using plasmids with inducible promoters such as that described in FIG. 10.  
     [0091] Optimization of oligonucleotide concentration. To determine the optimal concentration of oligonucleotide for the purpose of gene alteration, we test the ability of increasing concentrations of Hyg3T/74α to correct the mutation in pAURHYG(rep)GFP contained in yeast LSY678. We chose this assay system because our previous experiments indicated that it supports the highest level of correction. However, this same approach could be used to determine the optimal concentration of any given oligonucleotide. We test the ability of Hyg3T/74α to correct the mutation in pAURHYG(rep)GFP contained in yeast LSY678 over a range of oligonucleotide concentrations from 0-10.0 μg. As shown in Table 9, we observe that the correction efficiency initially increases with increasing oligonucleotide concentration, but then declines at the highest concentration tested.  
     [0092] Tables are attached hereto.  
               TABLE 6                          Correction of an insertion mutation in pAURHYG(ins)GFP by       HygGG/Rev, HygE3T/25 and HygE3T/74                                 Colonies on   Colonies on   Correction       Oligonucleotide Tested   Hygromycin   Aureobasidin (/10 5 )   Efficiency                                     HygGG/Rev   3   157   0.02       HygE3T/25   64   147   0.44       HygE3T/74   280   174   1.61       Kan70T   0   —   —                  
 
     [0093]               TABLE 7                          An oligonucleotide targeting the sense strand of the target sequence       corrects more efficiently.                             Colonies per               hygromycin plate                         Amount of Oligonucleotide (μg)   HygE3T/74   HygE3T/74α                                 0   0   0       0.6   24   128 (8.4x)*       1.2   69   140 (7.5x)*       2.4   62   167 (3.8x)*       3.6   29   367 (15x)*                             
     [0094]               TABLE 8                          Correction of a base substitution mutation is more efficient than correction       of a frame shift mutation.                         Oligonucleotide   Plasmid tested (contained in LSY678)                             Tested (5 μg)   pAURHYG(ins)GFP   pAURHYG(rep)GFP                                 HygE3T/74   72   277       HygE3T/74α   1464   2248       Kan70T   0   0                    
     [0095]               TABLE 9                          Optimization of oligonucleotide concentration in electroporated yeast cells.                                 Colonies on   Colonies on   Correction       Amount (μg)   hygromycin   aureobasidin (/10 5 )   efficiency                                     0   0   67   0       1.0   5   64   0.08       2.5   47   30   1.57       5.0   199   33   6.08       7.5   383   39   9.79       10.0   191   33   5.79                    
     EXAMPLE 3  
     Cultured Cell Manipulation  
     [0096] Although disclosure in this example is directed to use of stem cells or human blood cells and microinjection, the microinjection procedures may also be used with cultured plant cells or protoplasts using any plant species, including those disclosed herein. Mononuclear cells are isolated from human umbilical cord blood of normal donors using Ficoll Hypaque (Pharmacia Biotech, Uppsala, Sweden) density centrifugation. CD34+ cells are immunomagnetically purified from mononuclear cells using either the progenitor or Multisort Kits (Miltenyi Biotec, Auburn, Calif.). Lin − CD38 −  cells are purified from the mononuclear cells using negative selection with StemSep system according to the manufacturer&#39;s protocol (Stem Cell Technologies, Vancouver, Calif.). Cells used for microinjection are either freshly isolated or cryopreserved and cultured in Stem Medium (S Medium) for 2 to 5 days prior to microinjection. S Medium contains Iscoves&#39; Modified Dulbecc&#39;s Medium without phenol red (IMDM) with 100 μg/ml glutamine/penicillin/streptomycin, 50 mg/ml bovine serum albumin, 50 μg/ml bovine pancreatic insulin, 1 mg/ml human transferrin, and IMDM; Stem Cell Technologies), 40 μg/ml low-density lipoprotein (LDL; Sigma, St. Louis, Mo.), 50 mM HEPEs buffer and 50 μM 2-mercaptoethanol, 20 ng/ml each of thrombopoietin, flt-3 ligand, stem cell factor and human IL-6 (Pepro Tech Inc., Rocky Hill, N.J.). After microinjection, cells are detached and transferred in bulk into wells of 48 well plates for culturing.  
     [0097] 35 mm dishes are coated overnight at 4° C. with 50 μg/ml Fibronectin (FN) fragment CH-296 (Retronectin; TaKaRa Biomedicals, Panvera, Madison, Wis.) in phosphate buffered saline and washed with IMDM containing glutamine/penicillin/streptomycin. 300 to 2000 cells are added to cloning rings and attached to the plates for 45 minutes at 37° C. prior to microinjection. After incubation, cloning rings are removed and 2 ml of S Medium are added to each dish for microinjection. Pulled injection needles with a range of 0.22 μm to 0.3 μm outer tip diameter are used. Cells are visualized with a microscope equipped with a temperature controlled stage set at 37° C. and injected using an electronically interfaced Eppendorf Micromanipulator and Transjector. Successfully injected cells are intact, alive and remain attached to the plate post injection. Molecules that are flourescently labeled allow determination of the amount of oligonucleotide delivered to the cells.  
     [0098] For in vitro erythropoiesis from Lin − CD38 −  cells, the procedure of Malik, 1998 can be used. Cells are cultured in ME Medium for 4 days and then cultured in E Medium for 3 weeks. Erythropoiesis is evident by glycophorin A expression as well as the presence of red color representing the presence of hemoglobin in the cultured cells. The injected cells are able to retain their proliferative capacity and the ability to generate myeloid and erythoid progeny. CD34+ cells can convert a normal A (β A ) to sickle T (β S ) mutation in the β-globin gene or can be altered using any of the oligonucleotides of the invention herein for correction or alteration of a normal gene to a mutant gene. Alternatively, stem cells can be isolated from blood of humans having genetic disease mutations and the oligonucleotides of the invention can be used to correct a defect or to modify genomes within those cells.  
     [0099] Alternatively, non-stem cell populations of cultured cells can be manipulated using any method known to those of skill in the art including, for example, the use of polycations, cationic lipids, liposomes, polyethylenimine (PEI), electroporation, biolistics, calcium phosphate precipitation, or any other method known in the art.  
     [0100] Biolistic delivery of oligonucleotide into plant cells may be accomplished according to the following method. One milliliter of packed cell volume of plant cell suspensions are subcultured onto plates containing solid medium [with Murashige and Skoog salts from Gibco/BRL, 500 mg/liter Mes, 1 mg/liter thiamin, 100 mg/liter myo-inositol, 180 mg/liter KH2PO4, 2.21 mg/liter 2,4-dichlorophenoxyacetic acid (2,4-D), and 30 g/liter sucrose (pH 5.7) and having 8 g/liter agar-agar from Sigma added before autoclaving]. By using a helium-driven particle gun such as that from BioRad and following manufacturers directions, oligonucleotides may be introduced to cells after precipitation onto 1 micrometer or comparable gold microcarriers (Bio-Rad). To precipitate onto microcarriers, 35 microliters of a particle suspension (60 mg of microcarriers per ml of 100% ethanol) is transferred to a 1.5 ml microcentrifuge tube, which is agitated on a vortex mixer. Then 40 microliter of resuspended oligonucleotide (60 ng/microliter water) is added; then 75 microliter of ice-cold 2.5 M CaCl2 is added; then 75 microliter of ice-cold 0.1 M spermidine is added. The tube is mixed vigorously or a vortex mixer for 10 min at room temperature. The particles are allowed to settle for 10 min and are centrifuged at 11,750 g for 30 sec. The supernatant is removed and the particles are resuspended in 50 microliter of 100% ethanol. An aliquot of 10 microliter of the resuspended particles are applied to each macro-projectile which is used to bombard each plate once at 900 psi (1 psi=6.89 kPa) with a gap distance (distance from power source to macroprojectile) of 1 cm and a target distance (distance from microprojectile launch site to target material) of 10 cm.  
     [0101] An alternative method of delivery can be used as follows. Cultured cells are suspended in liquid N6 medium and then plated on a VWR Scientific glass fiber filter. About 0.4 microgram of oligonucleotide are precipitated with 15 microliter of 2.5 mM CaCl2 and 5 microliter of 0.1 M spermidine onto 25 microgram of 1.0 micrometer gold particles. Microprojectile bombardment is performed by using a Bio-Rad PDS-1000 He particle delivery system or comparable machine following manufacturers instructions. Alterations in oligonucleotide concentrations can be employed to determine the optimum concentration of oligonucleotide according to the procedures described herein for any particular oligonucleotide of the invention.  
     [0102] Alternatively, the oligonucleotide of the invention may be delivered to a plant cell by electroporation of a protoplast derived from a plant part. The protoplasts may be formed by enzymatic treatment of a plant part, particularly a leaf, according to techniques such as those in Gallois et al., Methods in Molecular Biology 55: 89-107 by Humana Press. Such conditions for electroporation use about 3×10 5  protoplasts in a total volume of about 0.3 ml with a concentration of oligonucleotide of between 0.6 to 4 microgram per ml.  
     EXAMPLE 4  
     Plant Cells  
     [0103] The oligonucleotides of the invention can also be used to repair or direct a mutagenic event in plants and animal cells. Although little information is available on plant mutations amongst natural cultivars, the oligonucleotides of the invention can be used to produce “knock out” mutations by modification of specific amino acid codons to produce stop codons (e.g., a CAA codon specifying Gln can be modified at a specific site to TAA; a AAG codon specifying Lys can be modified to UAG at a specific site; and a CGA codon for Arg can be modified to a UGA codon at a specific site). Such base pair changes will terminate the reading frame and produce a defective truncated protein, shortened at the site of the stop codon.  
     [0104] Alternatively, frameshift additions or deletions can be directed into the genome at a specific sequence to interrupt the reading frame and produce a garbled downstream protein. Such stop or frameshift mutations can be introduced to determine the effect of knocking out the protein in either plant or animal cells.  
     [0105] For introduction of a T-DNA, including the T-DNA in the plasmid of FIG. 11, into a plant cell,  Agrobacterium tumefaciens  is used. These techniques are routine standard techniques known in the art. For example, one method follows. We transform  A. tumefaciens  is transformed by electroporation (using a BioRad Gene Pulser™). Competent  A. tumefaciens  is prepared using a method similar to that of preparing competent  E. coli  by suspending a freshly grown culture three times in ice-cold water and a final resuspension in 10% glycerol. Electroporation conditions are a 0.2 cm gap cuvette at a setting of 25 μF,200 Ω and2.5 kV.  
     [0106] A. tumefaciens  containing a plasmid with a T-DNA is then used to introduce the T-DNA into a plant cell using routine standard techniques known in the art. For example, we transform Arabidopsis by vacuum infiltration or by dipping flowers in an Agrobacterium solution containing a surfactant, e.g. L-77. Seeds are then collected, grown and screened for presence of the T-DNA. Alternatively, Agrobacterium can be used to transform callus tissue and the callus tissue can then be used to regenerate transformed plants.  
     [0107] All publications and patent applications cited in this specification are herein incorporated by reference as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the appended claims.  
     [0108] Notes on the Tables Presented Below:  
     [0109] Each of the following tables presents, for the specified gene, a plurality of mutations that are known to confer a relevant phenotype and, for each mutation, the oligonucleotides that can be used to correct the respective mutation site-specifically in the genome according to the present invention.  
     [0110] The left-most column identifies each alteration or mutation and the phenotype that the alteration/mutation confers.  
     [0111] For most entries, the mutation/alteration is identified at both the nucleic acid and protein level. At the amino acid level, mutations are presented according to the following standard nomenclature. The centered number identifies the position of the mutated codon in the protein sequence; to the left of the number is the wild type residue and to the right of the number is the mutant codon. Terminator codons are shown as “TERM”. At the nucleic acid level, the entire triplet of the wild type and mutated codons is shown.  
     [0112] The middle column presents, for each mutation, four oligonucleotides capable of repairing the mutation site-specifically in the genome or in cloned DNA including DNA in artificial chromosomes, episomes, plasmids, or other types of vectors. The oligonucleotides of the invention, however, may include any of the oligonucleotides sharing portions of the sequence of the 121 base sequence. Thus, oligonucleotides of the invention for each of the depicted targets may be 18, 19, 20 up to about 121 nucleotides in length. Sequence may be added non-symmetrically.  
     [0113] All oligonucleotides are presented, per convention, in the 5′ to 3′ orientation. The nucleotide that effects the change in the genome is underlined and presented in bold.  
     [0114] The first of the four oligonucleotides for each mutation is a 121 nt oligonucleotide centered about the repair/altering nucleotide. The second oligonucleotide, its reverse complement, targets the opposite strand of the DNA duplex for repair/alteration. The third oligonucleotide is the minimal 17 nt domain of the first oligonucleotide, also centered about the repair/alteration nucleotide. The fourth oligonucleotide is the reverse complement of the third, and thus represents the minimal 17 nt domain of the second.  
     [0115] The third column of each table presents the SEQ ID NO: of the respective repair oligonucleotide.  
     EXAMPLE 5  
     Engineering Herbicide Resistant Plants  
     [0116] Chemical weed control is an important tool of modern agriculture and many herbicides have been developed for this purpose. Their use has resulted in substantial increases in the yields of many crops, including, for example, maize, soybeans, and cotton. Thus while the use of fertilizers and new high-yielding crop varieties have contributed greatly to the “green revolution,” chemical weed control has also been at the forefront of technological achievement.  
     [0117] Herbicides having broad-spectrum activity are particularly useful because they obviate the need for multiple herbicides targeting different classes of weeds. The problem with such herbicides is that they typically also affect crops which are exposed to the herbicide. One way to overcome this is to generate plants which are resistant to one or more broad-spectrum herbicides. Such herbicide-tolerant plants may reduce the need for tillage to control weeds, thereby effectively reducing soil erosion and can reduce the quantity and number of different herbicides applied in the field.  
     [0118] Common herbicides used, for example, include those that inhibit the enzyme 5-enolpyruvyl-3-phosphoshikimic acid synthase (EPSPS), for example N-phosphonomethyl-glycine (e.g. glyphosate), those that inhibit acetolactate synthase (ALS) activity, for example the sulfonylureas and related herbicides, and those that inhibit dihydropteroate synthase, for example methyl[(4-amino-phenyl)sulfonyl]carbamate (e.g. Asulam). Herbicide-tolerant plants can be produced by several methods, including, for example, introducing into the genome of the plant the ability to degrade the herbicide, the capacity to produce a higher level of the targeted enzyme, and/or expressing an herbicide-tolerant allele of the enzyme.  
     [0119] The attached tables disclose exemplary oligonucleotides base sequences which can be used to generate site-specific mutations in plant genes that confer herbicide resistance.  
                   TABLE 10                          Genome-Altering Oligos Conferring Glyphosate Resistance                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:                                     Glyphosate Resistance   AAGCGTCGGAGATTGTACTTCAACCCATTTAGAGAAATCTCCGGTC   1           EPSPS   TTATTAAGCTTCCTGCCTCCAAGTCTCTATCAAATCGGATCCTGC         Arabidopsis thaliana     TTCTCGCTGCTCTGTCTGAGGTATATATCAC       Gly97Ala   GTGATATATACCTCAGACAGAGCAGCGAGAAGCAGGATCCGATT   2       GGC-GCC   TGATAGAGACTTGGAGGCAGGAAGCTTAATAAGACCGGAGATTT           CTCTAATGGGTTGAAGTACAATCTCCGACGCTT           GCTTCCTG   C   CTCCAAGT   3           ACTTGGAG   G   CAGGAAGC   4               Glyphosate Resistance   AAGCTTCAGAGATTGTGCTTCAACCAATCAGAGAAATCTCGGGTC   5       EPSPS   TCATTAAGCTACCCGCATCCAAATCTCTCTCCAATCGGATCCTCC         Brassica napus     TTCTTGCCGCTCTATCTGAGGTACATATACT       Gly93AIa   AGTATATGTACCTCAGATAGAGCGGCAAGAAGGAGGATCCGATT   6       GGA-GCA   GGAGAGAGATTTGGATGCGGGTAGCTTAATGAGACCCGAGATTT           CTCTGATTGGTTGAAGCACAATCTCTGAAGCTT           GCTACCCG   C   ATCCAAAT   7           ATTIGGAT   G   CGGGTAGC   8               Glyphosate Resistance   AGCCCAACGAGATTGTGCTGCAACCCATCAAAGATATATCAGGC   9       EPSPS 1   ACTGTTAAATTGCCTGCTTCTAAATCCCTTTCCAATCGTATTCTCC         Nicotiana tabacum     TTCTTGCTGCCCTTTCTAAGGGAAGGACTGT       Gly95Ala   ACAGTCCTTCCCTTAGAAAGGGCAGCAAGAAGGAGAATACGATT   10       GGT-GCT   GGAAAGGGATTTAGAA   G   CAGGCAATTTAACAGTGCCTGATATATC           TTTGATGGGTTGCAGCACAATCTCGTIGGGCT           ATTGCCTG   C   TTCTAAAT   11           ATTTAGAA   G   CAGGCAAT   12               Glyphosate Resistance   ATTGTTTCCTTGGTACGAAATGTCCTCCTGTTCGAATTGTCAGCA   13       EPSPS 2   AGGGAGGCCTTCCCGCAGGGAAGGTAAAGCTCTCTGGATCAATT         Nicotiana tabacum     AGCAGCCAGTACTTGACTGCTCTGCTTATGGC       Gly62Ala   GCCATAAGCAGAGCAGTCAAGTACTGGCTGCTAATTGATCCAGA   14       GGA-GCA   GAGCTTTACCTTCCCT   G   CGGGAAGGCCTCCCTTGCTGACAATTC           GAACAGGAGGACATTTCGTACCAAGGAAACAAT           CCTTCCCG   C   AGGGAAGG   15           CCTTCCCG   C   GGGAAGG   16               Glyphosate Resistance   ATTGTTTCCTTGGCACTGACTGGCCACCTGTTCGTGTCAATGGAA   17       EPSPS   TCGGAGGGCTACCTG   C   TGGCAAGGTCAAGCTGTCTGGCTCCATC         Zea mays     AGCAGTCAGTACTTGAGTGCCTTGCTGATGGC       Gly168Ala   GCCATCAGCAAGGCACTCAAGTACTGACTGCTGATGGAGCCAGA   18       GGT-GCT   CAGCTTGACCTTGCCA   G   CAGGTAGCCCTCCGATTCCATTGACAC           GAACAGGTGGGCAGTCAGTGCCAAGGAAACAAT           GCTACCTG   C   TGGCAAGG   19           CCTTGCCA   G   CAGGTAGC   20               Glyphosate Resistance   ACTGTTTCCTTGGCACTGAATGCCCACCTGTTCGTGTCAAGGGA   21       EPSPS   ATTGGAGGACTTCCTG   C   TGGCAAGGTTAAGCTCTCTGGTTCCAT         Cryza sativa     CAGCAGTCAGTACTTGAGTGCCTTGCTGATGGC       Gly115Ala   GCCATCAGCAAGGCACTCAAGTACTGACTGCTGATGGAACCAGA   22       GGT-GCT   GAGCTTAACCTTGCCAGCAGGAAGTCCTCCAATTCCCTTGACAC           GAACAGGTGGGCATTCAGTGCCAAGGAAACAGT           ACTTCCTG   C   TGGCAAGG   23           CCTTGCCA   G   CAGGAAGT   24               Glyphosate Resistance   AGCCTTCTGAGATAGTGTTGCAACCCATTAAAGAGATTTCAGGCA   25       EPSPS   CTGTTAAATTGCCTGCCTCTAAATCATTATCTAATAGAATTCTCCT         Petunia x hybrida     TCTTGCTGCCTTATCTGAAGGMCAACTGT       Gly93Ala   ACAGTTGTTCCTTCAGATAAGGCAGCAAGAAGGAGAATTCTATTA   26       GGC-GCC   GATAATGATTTAGAGGCAGGCAATTTAACAGTGCCTGAAATCTCT           TTAATGGGTTGCAACACTATCTCAGAAGGCT           ATTGCCTG   CCTCTAAAT   27           ATTTAGAG   G   CAGGCAAT   28               Glyphosate Resistance   AACCCCATGAGATTGTGCTAGNACCCATCAAAGATATATCTGGTA   29       EPSPS   CTGTTAAATTACCCG   C   TTCGAAATCCCTTTCCAATCGTATTCTCCT         Lycopersicon     TCTTGCTGCCCTTTCTGAGGGAAGGACTGT         esculentum     ACAGTCCTTCCCTCAGAAAGGGCAGCAAGAAGGAGAATACGATT   30       Gly97Ala   GGAAAGGGATTTCGAA   G   CGGGTAATTTAACAGTACCAGATATATC       GGT-GCT   TTTGATGGGTNCTAGCACAATCTGATGGGGTT           ATTACCCG   C   TTCGAAAT   31           ATTTCGAA   G   CGGGTAAT   32               Glyphosate Resistance   ATTGTTTCCTTGGCACTGACTGCCCACCTGTTCGKATCAACGGGA   33       EPSPS   TTGGAGGGCTACCTGCTGGCAAGGTTAAGCTGTCTGGTTCCAIT         Lolium rigidum     AGCAGCCAATACTTGAGTTCCTTGCTGATGGC       Gly107Ala   GCCATCAGCAAGGAACTCAAGTATTGGCTGCTGATGGAACCAGA   34       GGT-GCT   CAGCTTAACCTTGCCA   G   CAGGTAGCCCTCCAATGCCGTTGATCG           AACAGGTGGGCAGTCAGTGCCAAGGAAACAAT           GCTACCTG   C   TGGCAAGG   35           CCTTGCCA   G   CAGGTAGC   36                  
 
     [0120]                   TABLE 11                          Genome-Altering Oligos Conferring Imidazolinone           and Sulfonylurea Herbicide Resistance                             Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:                                     Sulfonylurea   AGCGGATTAGCCGATGCGTTGTTAGATAGTGTTCCTCTTGTAGCA   37           Resistance   ATCACAGGACAAGTC   T   CTCGTCGTATGATTGGTACAGATGCGTTT       ALS   CAAGAGACTCCGATTGTTGAGGTAACGCGTT       Arabidopsis thaliana   AACGCGTTACCTCAACAATCGGAGTCTCTTGAAACGCATCTGTAC   38       Pro197Ser   CAATCATACGACGAG   A   GACTTGTCCTGTGATTGCTACAAGAGGAA       CCT-TCT   CACTATCTAACAACGCATCGGCTAATCCGCT           GACAAGTCTC   T   CGTCGT   39           ACGACGAG   A   GACTTGTC   40               Sulfonylurea   AGCGGATTAGCCGATGCGTTGTTAGATAGTGTTCCTCTTGTAGCA   41       Resistance   ATCACAGGACAAGTCC   AG   CGTCGTATGATTGGTACAGATGCGTTT       ALS   CAAGAGACTCCGATTGTTGAGGTAACGCGTT         Arabidopsis thaliana     AACGCGTTACCTCAACAATCGGAGTCTCTTGAAACGCATCTGTAC   42       Pro197GLN   CAATCATACGACG   C   TGGACTTGTCCTGTGATTGCTACAAGAGGAA       CCT-CAG   CACTATCTAACAACGCATCGGCTAATCCGCT           ACAAGTCC   AG   CGTCGTC   43           TACGACG   CT   GGACTTGT   44               Sulfonylurea   AGCGGATTAGCCGATGCGTTGTTAGATAGTGTTCCTCTTGTAGCA   45       Resistance   ATCACAGGACAAGTCC   AA   CGTCGTATGATTGGTACAGATGCGTTT       ALS   CAAGAGACTCCGATTGTTGAGGTAACGCGTT         Arabidopsis thaliana     AACGCGTTACCTCAACAATCGGAGTCTCTTGAAACGCATCTGTAC   46       Pro197GLN   CAATCATACGACG   TT   GGACTTGTCCTGTGATTGCTACAAGAGGAA       CCT-CAA   CACTATCTAACAACGCATCGGCTAATCCGCT           ACAAGTCC   AA   CGTCGTA   47           TACGACG   TT   GGACTTGT   48               Imidazolinone   GACCTTACCTGTTGGATGTGATTTGTCCGCACCAAGAACATGTGT   49       Resistance   TGCCGATGATCCCGA   AC   GGTGGCACTTTCAACGATGTCATAACGG       ALS   AAGGAGATGGCCGGATTAAATACTGAGAGAT         Arabidopsis thaliana     ATCTCTCAGTATTTAATCCGGCCATCTCCTTCCGTTATGACATCGT   50       Ser653Asn   TGAAAGTGCCACC   GT   TCGGGATCATCGGCAACACATGTTCTTGGT       AGT-AAC   GCGGACAAATCACATCCAACAGGTAAGGTC           GATCCCGA   AC   GGTGGCA   51           TGCCACC   GT   TCGGGATC   52               Imidazolinone   GACCTTACCTGTTGGATGTGATTTGTCCGCACCAAGAACATGTGT   53       Resistance   TGCCGATGATCCCGA   AT   GGTGGCACTTTCAACGATGTCATAACGG       ALS   AAGGAGATGGCCGGATTAAATACTGAGAGAT         Arabidopsis thaliana     ATCTCTCAGTATTTAATCCGGCCATCTCCTTCCGTTATGACATCGT   54       Ser653Asn   TGAAAGTGCCACC   AT   TCGGGATCATCGGCAACACATGTTCTTGGT       AGT-AAT   GCGGACAAATCACATCCAACAGGTAAGGTC           GATCCCGA   AT   GGTGGCA   55           TGCCACC   AT   TCGGGATC   56               Sulfonylurea   TCCGCGCTCGCCGACGCGCTGCTCGACTCCGTCCCGATGGTCGC   57       Resistance   CATCACGGGCCAGGTC   T   CCCGCCGCATGATCGGCACCGACGCCT       ALS   TCCAGGAGACGCCCATAGTCGAGGTCACCCGCT         Oryza saliva     AGCGGGTGACCTCGACTATGGGCGTCTCCTGGAAGGCGTCGGTG   58       Pro171Ser   CCGATCATGCGGCGGG   A   GACCTGGCCCGTGATGGCGACCATCG       CCC-TCC   GGACGGAGTCGAGCAGCGCGTCGGCGAGCGCGGA           GCCAGGTC   T   CCCGCCGC   59           GCGGCGGG   A   GACCTGGC   60               Sulfonylurea   CCGCGCTCGCCGACGCGCTGCTCGACTCCGTCCCGATGGTCGCC   61       Resistance   ATCACGGGCCAGGTCC   AA   CGCCGCATGATCGGCACCGACGCCTT       ALS   CCAGGAGACGCCCATAGTCGAGGTCACCCGCTC         Oryza saliva     GAGCGGGTGACCTCGACTATGGGCGTCTCCTGGAAGGCGTCGGT 62       Pro171Gln   GCCGATCATGCGGCG   TT   GGACCTGGCCCGTGATGGCGACCATCG       CCC-CAA   GGACGGAGTCGAGCAGCGCGTCGGCGAGCGCGG           CCAGGTCC   AA   CGCCGCA   63           TGCGGCG   TT   GGACCTGG   64               Sulfonylurea   CCGCGCTCGCCGACGCGCTGCTCGACTCCGTCCCGATGGTCGCC   65       Resistance   ATCACGGGCCAGGTCC   AG   CGCCGCATGATCGGCACCGACGCCTT       ALS   CCAGGAGACGCCCATAGTCGAGGTCACCCGCTC         Oryza saliva     GAGCGGGTGACCTCGACTATGGGCGTCTCCTGGAAGGCGTCGGT   66       Pro171Gln   GCCGATCATGCGGCG   CT   GGACCTGGCCCGTGATGGCGACCATCG       CCC-CAG   GGACGGAGTCGAGCAGCGCGTCGGCGAGCGCGG           CCAGGTCC   AG   CGCCGCA   67           TGCGGCG   CT   GGACCTGG   68               Imidazolinone   GGCCATACTTGTTGGATATCATCGTCCCGCACCAGGAGCATGTGC   69       Resistance   TGCCTATGATCCCAA   A   TGGGGGCGCATTCAAGGACATGATCCTGG       ALS   ATGGTGATGGCAGGACTGTGTATTAATCTAT         Oryza saliva     ATAGATTAATACACAGTCCTGCGATCACCATCCAGGATCATGTCCT   70       Ilee627Asn   TGAATGCGCCCCCA   T   TTGGGATCATAGGCAGCACATGCTCCTGGT       ATT-AAT   GCGGGACGATGATATCCAACAAGTATGGCC           GATCCCAA   A   TGGGGGCG   71           CGCCCCCA   T   TTGGGATC   72               Sulfonylurea   TCCGCGCTCGCCGACGCGCTGCTCGATTCCGTCCCCATGGTCGC   73       Resistance   CATCACGGGACAGGTG   T   CGCGACGCATGATTGGCACCGACGCCT       ALS   TCCAGGAGACGCCCATCGTCGAGGTCACCCGCT         Zea mays     AGCGGGTGACCTCGACGATGGGCGTCTCCTGGAAGGCGTCGGT   74       Pro165Ser   GCCAATCATGCGTCGCG   A   CACCTGTCCCGTGATGGCGACCATGG       CCG-TCG   GGACGGAATCGAGCAGCGCGTCGGCGAGCGCGGA           GACAGGTG   T   CGCGACGC   75           GCGTCGCG   A   CACCTGTC   76               Sulfonylurea   CCGCGCTCGCCGACGCGCTGCTCGATTCCGTCCCCATGGTCGCC   77       Resistance   ATCACGGGACAGGTGC   A   GCGACGCATGATTGGCACCGACGCCTT       ALS   CCAGGAGACGCCCATCGTCGAGGTCACCCGCTC         Zea mays     GAGCGGGTGACCTCGACGATGGGCGTCTCCTGGAAGGCGTCGG   78       Pro165Gln   TGCCAATCATGCGTCGC   T   GCACCTGTCCCGTGATGGCGACCATG       CCG-CAG   GGGACGGAATCGAGCAGCGCGTCGGCGAGCGCGG           ACAGGTGC   A   GCGACGCA   79           TGCGTCGC   T   GCACCTGT   80               Imidazolinone   GGCCGTACCTCTTGGATATAATCGTCCCACACCAGGAGCATGTGT   81       Resistance   TGCCTATGATCCCTA   AT   GGTGGGGCTTTCAAGGATATGATCCTGG       ALS   ATGGTGATGGCAGGACTGTGTACTGATCTAA         Zea mays     TTAGATCAGTACACAGTCCTGCCATCACCATCCAGGATCATATCCT   82       Ser621Asn   TGAAAGCCCCACC   AT   TAGGGATCATAGGCAACACATGCTCCTGGT       AGT-AAT   GTGGGACGATTATATCCAAGAGGTACGGCC           GATCCCTA   AT   GGTGGGG   83           CCCCACC   AT   TAGGGATC   84               Imidazolinone   GGCCGTACCTCTTGGATATAATCGTCCCACACCAGGAGCATGTGT   85       Resistance   TGCCTATGATCCCTA   AC   GGTGGGGCTTTCAAGGATATGATCCTGG       ALS   ATGGTGATGGCAGGACTGTGTACTGATCTAA         Zea mays     TTAGATCAGTACACAGTCCTGCCATCACCATCCAGGATCATATCCT   86       Ser621Asn   TGAAAGCCCCACC   GT   TAGGGATCATAGGCAACACATGCTCCTGGT       AGT-AAC   GTGGGACGATTATATCCAAGAGGTACGGCC           GATCCCTA   AC   GGTGGGG   87           CCCCACC   GT   TAGGGATC   88               Sulfonylurea   TCCGCGCTCGCCGACGCCGTCCTCGACTCCATCCCCATGGTGGC   89       Resistance   CATCACGGGGCAGGTC   T   CGCGCCGCATGATCGGCACGGACGCCT       ALS   TCCAGGAGACGCCCATCGTCGAGGTCACCCGCT         Lolium multiflorum     AGCGGGTGACCTCGACGATGGGCGTCTCCTGGAAGGCGTCCGTG   90       Pro167Ser   CCGATCATGCGGCGCG   A   GACCTGCCCCGTGATGGCCACCATGG       CCG-TCC   GGATGGAGTVGAGGAGGGCCTCGGCGACCCCCCA           GGCAGGTC   T   CGCGCCGC   91           GCGGCGCG   A   GACCTGCC   92               Sulfonylurea   CCGCGCTCGCCGACGCCCTCCTCGACTCCATCCCCATGGTGGCC   93       Resistance   ATCACGGGGCAGGTCC   A   GCGCCGCATGATCGGCACGGACGCCTT       ALS   CCAGGAGACGCCCATCGTCGAGGTCACCCGCTC         Lolium multiflorum     GAGCGGGTGACCTCGACGATGGGCGTCTCCTGGAAGGCGTCCGT   94       Pro167Gln   GCCGATCATGCGGCGC   T   GGACCTGCCCCGTGATGGCCACCATGG       CCG-CAG   GGATGGAGTCGAGGAGGGCGTCGGCGAGCGCGG           GCAGGTCC   A   GCGCCGCA   95           TGCGGCGC   T   GGACCTGC   96               Imidazolinone   CTGGGCCATACTTGTTGGATATCATCGTCCCTCACCAGGAGCATG   97       Resistance   TGCTGCCTATGATCCCTA   A   CGGTGGTGCTTTCAAGGACATTATCA       ALS   TGGAAGGTGATGGCAGGATTTCGTATTAAAC         Lolium multiflorum     GTTTAATACGAAATCCTGCCATCACCTTCCATGATAATGTCGTTGA   98       Ser623Asn   AAGCACCACCG   T   TAGGGATCATAGGCAGCACATGCTCCTGGTGA       AGC-AAC   GGGACGATGATATCCAACAAGTATGGCCCAG           GATCCCTA   A   CGGTGGTG   99           CACCACCG   T   TAGGGATC   100               Sulfonylurea   TCCGCGCTCGCCGACGGTCTCCTCGACTCCATCGCCATGGTCGC   101       Resistance   CATCACGGGCCAGGTC   T   CACGCCGCATGATCGGCACGGACGCGT       ALS   TCCAGGAGACGCCCATAGTGGAGGTCACGCGCT         Hordeum vulgare     AGCGCGTGACCTCCACTATGGGCGTCTCCTGGAACGCGTCCGTG   102       Pro68Ser   CGGATCATGCGGCGTG   A   GACCTGGCCCGTGATGGCGACCATGG       CCA-TCA   GGATGGAGTCGAGGAGAGCGTCGGCGAGCGCGGA           GCCAGGTC   T   CACGCCGC   103           GCGGCGTG   A   GACCTGGC   104               Sulfonyurea   CCGCGCTCGCCGACGCTCTCCTCGACTCCATCCCCATGGTCGCC   105       Resistance   ATCACGGGCCAGGTCC   A   ACGCCGCATGATCGGCACGGACGCGTT       ALS   CCAGGAGACGCCCATAGTGGAGGTCACGCGCTC         Hordeum vulgare     GAGCGCGTGACCTCCACTATGGGCGTCTCCTGGAACGCGTCCGT   106       Pro68Gln   GCCGATCATGCGGCGT   T   GGACCTGGCCCGTGATGGCGACCATGG       CCA-CAA   GGATGGAGTCGAGGAGAGCGTCGGCGAGCGCGG           CCAGGTCC   A   ACGCCGCA   107           TGCGGCGT   T   GGACCTGG   108               Imidazolinone   CCCAGGGCCGTACCTGCTGGATATCATTGTCCCGCATCAGGAGC   109       Resistance   ACGTGCTGCCTATGATCCCAA   A   CGGTGGTGCTTTCAAGGACATGA       ALS   TCATGGAGGGTGATGGCAGGACCTCGTACTGA         Hordeum vulgare     TCAGTACGAGGTCCTGCCATTCACCCTCCATGATCATGTCCTTGAA   110       Ser524Asn   AGCACCACCG   T   TTGGGATCATAGGCAGCACGTGCTCCTGATGCG       AGC-AAC   GGACAATGATATCCAGCAGGTACGGCCCTGGG           GATCCCAA   A   CGGTGGTG   111           CACCACCG   T   TTGGGATC   112               Sulfonylurea   AGTGGTCTCGCTGATGCAATGCTCGATAGTATCCCTCTCGTGGCG   113       Resistance   ATCACTGGTCAAGTC   T   CTCGTCGGATGATCGGTACCGATGCTTTC       ALS   CAGGAAACTCCAATTGTTGAGGTAACAAGGT         Gossypium hirsutum     ACCTTGTTACCTCAACAATTGGAGTTTCCTGGAAAGCATCGGTAC   114       Pro186Ser   CGATCATCCGACGAG   A   GACTTGACCAGTGATCGCCACGAGAGGG       CCT-TCT   ATACTATCGAGCATTGCATCAGCGAGACCACT           GTCAAGTC   T   CTCGTCGG   115           CCGACGAG   A   GACTTGAC   116               Sulfonylurea   GTGGTCTCGCTGATGCAATGGTCGATAGTATCCCTCTCGTGGCGA   117       Resistance   TCACTGGTCAAGTCC   AA   CGTCGGATGATCGGTACCGATGCTTTCC       ALS   AGGAAACTCCAATTGTTGAGGTAACAAGGTC         Gossypium hirsutum     GACCTTGTTACCTCAACAATTGGAGTTICCTGGAAAGCATCGGTA   118       Pro186Gln   CCGATCATCCGACG   TT   GGACTTGACCAGTGATCGCCACGAGAGG       CCT-CAA   GATACTATCGAGCATTGCATCAGCGAGACCAC           TCAAGTCC   AA   CGTCGGA   119           TCCGACG   TT   GGACTTGA   120               Sulfonylurea   GTGGTCTCGCTGATGCAATGCTCGATAGTATCCCTCTCGTGGCGA   121       Resistance   TCACIGGTCAAGTCC   AG   CGTCGGATGATCGGTACCGATGCTTTCC       ALS   AGGAAACTCCAATTGTTGAGGTAACAAGGTC         Gossypium hirsutum     GACCTTGTTACCTCAACAATTGGAGTTTCCTGGAAAGCATCGGTA   122       Pro186Gln   CCGATCATCCGACG   CT   GGACTTGACCAGTGATCGCCACGAGAGG       CCT-CAG   GATACTATCGAGCATTGCATCAGCGAGACCAC           TCAAGTCC   AG   CGTCGGA   123           TCCGACG   CT   GGACTTGA   124               Imidazolinone   GACCTTACTTGTTGGATGTGATTGTCCCACATCAAGAACATGTCCT   125       Resistance   GCCTATGATCCCCA   A   TGGAGGCGCTTTCAAAGATGTGATCACAGA       ALS   GGGTGATGGAAGAACACAATATTGACCTCA         Gossypium hirsutum     TGAGGTCAATATTGTGTTCTTCCATCACCCTCTGTGATCACATCTT   126       Ser642Asn   TGAAAGCGCCTCCA   T   TGGGGATCATAGGCAGGACATGTTCTTGAT       AGT-AAT   GTGGGACAATCACATCCAACAAGTAAGGTC           GATCCCCA   A   TGGAGGCG   127           CGCCTCCA   T   TGGGGATC   128               Sulfonylurea   TCTGGTCTTGCTGATGCACTTCTTGACTCAGTCCCTCTTGTCGCCA   129       Resistance   TTACTGGGCAAGTT   T   CCCGGCGTATGATTGGTACTGATGCTTTTCA       ALS   AGAGACTCCAATTGTTGAGGTAACTCGAT         Amaranthus     ATCGAGTTACCTCAACAATTGGAGTCTCTTGAAAAGCATCAGTACC   130         retroflexus     AATCATACGCCGGG   A   AACTTGCCCAGTAATGGCGACAAGAGGGA       Pro192Ser   CTGAGTCAAGAAGTGCATCAGCAAGACCAGA       CCC-TCC   GGCAAGTT   T   CCCGGCGT   131           ACGCCGGG   A   AAGTTGCC   132               Sulfonylurea   CTGGTCTTGCTGATGCACTTCTTGACTCAGTCCCTCTTGTCGCCAT   133       Resistance   TACTGGGCAAGTTC   AA   CGGCGTATGATTGGTACTGATGCTTTTCA       ALS   AGAGACTCCAATTGTTGAGGTAACTCGATC         Amaranthus     GATCGAGTTACCTCAACAATTGGAGTCTCTTGAAAAGCATCAGTAC   134         retroflexus     CAATCATACGCCG   TT   GAACTTGCCCAGTAATGGCGACAAGAGGGA       Pro192Gln   CTGAGTCAAGAAGTGCATCAGCAAGACCAG       CCC-CAA   GCAAGTTC   AA   CGGCGTA   135           TACGCCG   TT   GAACTTGC   136               Sulfonylurea   CTGGTCTTGCTGATGCACTTCTTGACTCAGTCCCTCTTGTCGCCAT   137       Resistance   TACTGGGCAAGtTC   AG   CGGCGTATGATTGGTACTGATGCTTTTCA       ALS   AGAGACTCCAATTGTTGAGGTAACTCGATC         Amaranthus     GATCGAGTTACCTCAACAATTGGAGTCTCTTGAAAAGCATCAGTAC   138         retroflexus     CAATCATACGCCG   CT   GAACTTGCCCAGTAATGGCGACAAGAGGG       Pro192Gln   ACTGAGTCAAGAAGTGCATCAGCAAGACCAG       CCC-CAG   GCAAGTTC   AG   CGGCGTA   139           TACGCCG   CT   GAACTTGC   140               Imidazolinone   GACCGTATCTTGCTGGATGTTAATCGTACCACATCAGGAGCATGTGC   141       Resistance   TGCCTAIGATCCCTA   A   CGGTGCCGCCTTCAAGGACACCATAACAG       ALS   AGGGTGATGGAAGAAGGGGTTATTAGTTGGT         Amaranthus     ACCAACTAATAAGCCCTTCTTCCATTCACCCTCTGTTATGGTGTCCT   142         retroflexus     TGAAGGCGGCACCG   T   TAGGGATCATAGGCAGCACATGCTCCTGA       Ser652Asn   TGTGGTACGATTACATCCAGCAGATACGGTC       AGC-AAC   GATCCCTA   A   CGGTGCCG   143           CGGCACCG   T   TAGGGATC   144               Sulfonylurea   AGCGGCCTCGCTGACGCGCTACTGGATAGCGTCCCCATTGTTGC   145       Resistance   TATAACAGGTCAAGTG   T   CACGTAGGATGATAGGTACTGATGCTTTT       ALS 1   CAGGAAACTCCTATTGTITGAGGTAACTAGAT         Nicotiana tabacum     ATCTAGTTACCTCAACAATAGGAGTTTCCTGAAAAGCATCAGTACC   146       Pro194Ser   TATCATCCTACGTG   A   CACTTGACCTGTTATAGCAACAATGGGGAC       CCA-TCA   GCTATCCAGTAGCGCGTCAGCGAGGCCGCT           GTCAAGTG   T   CACGTAGG   147           CCTACGTG   A   CACTTGAC   148               Sulfonylurea   GCGGCCTCGCTGACGCGCTACTGGATAGCGTCCCCATTGTTGCT   149       Resistance   ATAACAGGTCAAGTGC   AA   CGTAGGATGATAGGTACTGATGCTTTT       ALS 1   CAGGAAACTCCTATTGTTGAGGTAACTAGATC         Nicotiana tabacum     GATCTAGTTACCTCAACAATAGGAGTTTCCTGAAAAGCATCAGTAC   150       Pro194Gln   CTATCATCCTACGT   T   GCACTTGACCTGTTATAGCAACAATGGGGA       CCA-CAA   CGCTATCCAGTAGCGCGTCAGCGAGGCCGC           TCAAGTGC   A   ACGTAGGA   151           TCCTACGT   T   GCACTTGA   152               Imidazolinone   GGCCATACTTGTTGGATGTGATTGTACCTCATCAGGAACATGTTTT   153       Resistance   ACCTATGATTCCCA   A   TGGCGGAGCTTTCAAAGATGTGATCACAGA       ALS 1   GGGTGACGGGAGAAGTTCCTATTGAGTTTG         Nicotiana tabacum     CAAACTGAATAGGAACTTCTCCCGTCACCCTCTGTGATCACATCTT   154       Ser650Asn   TGAAAGCTCCGCCA   T   TGGGAATCATAGGTAAAACATGTTCCTGAT       AGT-AAT   GAGGTACAATCACATCCAACAAGTATGGCC           GATTCCCA   A   TGGCGGAG   155           CTCCGCCA   T   TGGGAATC   156               Sulfonylurea   AGTGGCCTCGCGGACGCCCTACTGGATAGCGTCCCCATTGTTGC   157       Resistance   TATAACCGGTCAAGTG   T   CACGTAGGATGATCGGTACTGATGCTTT       ALS 2   TCAGGAAACTCCGATTGTTGAGGTAACTAGAT         Nicotiana tabacum     ATCTAGTTACCTCAACAATCGGAGTTTCCTGAAAAGCATCAGTACC   158       Pro191Ser   GATCATCCTACGTG   A   CACTTGACCGGTTATAGCAACAATGGGGAC       CCA-TCA   GCTATCCAGTAGGGCGTCCGCGAGGCCACT           GICAAGTG   T   CACGTAGG   159           CCTACGTG   A   CACTTGAC   160               Sulfonylurea   GTGGCCTCGCGGACGCCCTACTGGATAGCGTCCCCATTGTTGCT   161       Resistance   ATAACCGGTCAAGTGC   A   ACGTAGGATGATCGGTACTGATGCTTTT       ALS 2   CAGGAAACTCCGATTGTTGAGGTAACTAGATC         Nicotiana tabacum     GATCTAGTTACCTCAACAATCGGAGTTTCCTGAAAAGCATCAGTAC   162       Pro191Gln   CGATCATCCTACGT   T   GCACTTGACCGGTTATAGCAACAATGGGGA       CCA-CAA   CGCTATCCAGTAGGGCGTCCGCGAGGCCAC           TCAAGTGC   A   ACGTAGGA   163           TCCTACGT   T   GCACTTGA   164               Imidazolinone   GGCCATACTTGTTGGATGTGATTGTACCTCATCAGGAACATGTTCT   165       Resistance   ACCTATGATTCCCA   A   TGGCGGGGCTTTCAAAGATGTGATCACAGA       ALS 2   GGGTGACGGGAGAAGTTCCTATTGACTTTG         Nicotiana tabacum     CAAAGTCAATAGGAACTTCTCCCGTCACCCTCTGTGATCACATCTT   166       Ser647Asn   TGAAAGCCCCGCCA   T   TGGGAATCATAGGTAGAACATGTTCCTGAT       AGT-AAT   GAGGTACAATCACATCCAACAAGTATGGCC           GATTCCCA   A   TGGCGGGG   167           CCCCGCCA   T   TGGGAATC   168               Sulfonylurea   AGTGGTCTTGCTGATGCTTTATTAGACAGTGTTCCAATGGTTGCTA   169       Resistance   TTACTGGTCAAGTT   T   CCAGGAGAATGATTGGAACAGATGCGTTTC       ALS   AAGAAACCCCTATTGTTGAGGTAACACGTT       Xanthium spp.   AACGTGTTACCTCAACAATAGGGGTTTCTTGAAACGCATCTGTTCC   170       Pro175Ser   AATCATTCTCCTGG   A   AACTTGACCAGTAATAGCAACCATTGGAACA       CCC-TCC   CTGTCTAATAAAGCATCAGCAAGACCACT           GTCAAGTT   T   CCAGGAGA   171           TCTCCTGG   A   AACTTGAC   172               Sulfonylurea   GTGGTCTTGCTGATGCTTTATTAGACAGTGTTCCAATGGTTGCTAT   173       Resistance   TACTGGTCAAGTTC   AA   AGGAGAATGATTGGAACAGATGCGTTTCA       ALS   AGAAACCCCTATTGTTGAGGTAACACGTTC       Xanthium spp.   GAACGTGTTACCTCAACAATAGGGGTTTCTTGAAACGCATCTGTTC   174       Pro175Gln   CAATCATTCTCCT   TT   GAACTTGACCAGTAATAGCAACCATTGGAAC       CCC-CAA   ACTGTCTAATAAAGCATCAGCAAGACCAC           TCAAGTTC   AA   AGGAGAA   175           TTCTCCT   TT GAACTTGA     176               Sulfonylurea   GTGGTCTTGCTGATGCTTTATTAGACAGTGTTCCAATGGTTGCTAT   177       Resistance   TACTGGTCAAGTTC   AG AGGAGAATGATTGGAACAGATGCGTTTCA         ALS   AGAAACCCCTATTGTTGAGGTAACACGTTC       Xanthium spp.   GAACGTGTTACCTCAACAATAGGGGTTTCTTGAAACGCATCTGTTC 178       Pro175Gln   CAATCATTCTCCT   CT   GAACTTGACCAGTAATAGCAACCATTGGAAC       CCC-CAG   ACTGTCTAATAAAGCATCAGCAAGACCAC           TCAAGTTC   AG   AGGAGAA   179           TTCTCCT   CT   GAACTTGA   180               Imidazolinone   GGGCCTTACTTGTTGGATGTGATCGTGCCCCATCAAGAACATGTG   181       Resistance   TTGCCCATGATCCCG   AA   TGGTGGAGGTTTCATGGATGTGATCACC       ALS   GAAGGCGACGGCAGAATGAAATATTGAGCTT       Xanthium spp.   AAGCTCAATATTTCATTCTGCCGTCGCCTTCGGTGATCACATCCAT   182       Ala631Asn   GAAACCTCCACCA   TT   CGGGATCATGGGCAACACATGTTCTTGATG       GCT-AAT   GGGCACGATCACATCCAACAAGTAAGGCCC           TGATCCCG   AA   TGGTGGA   183           TCCACCA   TT   CGGGATCA   184               Sulfonylurea   TCCGGGTTTGCTGATGCTTTGCTCGATTCCGTTCCACTGGTGGCG   185       Resistance   ATCACGGGGCAGGTG   T   CGCGGCGAATGATTGGGACGGATGCTTT       ALS   TCAGGAGACTCCTATTGTTGAGGTAACACGGT         Bassia scoparia     ACCGTGTTACCTCAACAATAGGAGTCTCCTGAAAAGCATCCGTCC   186       Pro189Ser   CAATCATTCGCCGCG   A   CACCTGCCCCGTGATCGCCACCAGTGGA       CCG-TCG   ACGGAATCGAGCAAAGCATCAGCAAACCCGGA           GGCAGGTG   T   CGCGGCGA   187           TCGCCGCG   A   CACCTGCC   188               Sulfonylurea   CCGGGTTTGGTGATGCTTTGCTCGATTCCGTTCCACTGGTGGCGA   189       Resistance   TCACGGGGCAGGTGC   A   GCGGCGAATGATTGGGACGGATGCTTTT       ALS   CAGGAGACTCCTATTGTTGAGGTAACACGGTC         Bassia scoparia     GACCGTGTTACCTCAACAATAGGAGTCTCCTGAAAAGCATCCGTC   190       Pro189Gln   CCAATCATTCGCCGC   T   GCACCTGCCCCGTGATCGCCACCAGTGG       CCG-CAG   AACGGAATCGAGCAAAGCATCAGCAAACCCGG           GCAGGTGC   A   GCGGCGAA   191           TTCGCCGC   T   GCAGCTGC   192               Imidazolinone   GACCTTACCTGCTTGATGTGATTGTACCTCATCAGGAGCATGTGC   193       Resistance   TGCCTATGATTCCTA   A   TGGTGCAGCCTTCAAGGATATCATTAACGA       ALS   AGGTGATGGAAGAACAAGTTATTGATGTTC         Bassia scoparia     GAACATCAATAACTTGTTCTTCCATCACCTTCGTTAATGATATCCTT   194       Ser649Asn   GAAGGCTGCACCA   T   TAGGAATCATAGGCAGCACATGCTCCTGATG       AGT-AAT   AGGTACAATCACATCAAGCAGGTAAGGTC           GATTCGTA   A   TGGTGCAG   195           CTGCACCA   T   TAGGAATC   196               Sulfonylurea   AGCGGGTTAGCAGACGCGATGCTTGACAGTGTTCCTCTTGTCGCC   197       Resistance   ATTACAGGACAGGTC   T   CTCGCCGGATGATCGGTACTGACGCCTTC       ALS 1   CAAGAGACACCAATCGTTGAGGTAACGAGGT         Brassica napus     ACCTCGTTACCTCAACGATTGGTGTCTCTTGGAAGGCGTCAGTAC   198       Pro182Ser   CGATCATCCGGCGAG   A   GACCTGTCCTGTAATGGCGACAAGAGGA       CCT-TCT   ACACTGTCAAGCATCGCGTCTGCTAACCCGCT           GACAGGTC   T   CTCGCCGG   199           CCGGCGAG   A   GACCTGTC   200               Sulfonylurea   GCGGGTTAGCAGACGCGATGCTTGACAGTGTTCCTCTTGTCGCCA   201       Resistance   TTACAGGACAGGTCC   AA   CGCCGGATGATCGGTACTGACGCCTTC       ALS 1   CAAGAGACACCAATCGTTGAGGTAACGAGGTC         Brassica napus     GACCTCGTTACCTCAACGATTGGTGTCTCTTGGAAGGCGTCAGTA   202       Pro182Gln   CCGATCATCCGGCG   TT   GGACCTGTCCTGTAATGGCGACAAGAGG       CCT-CAA   AACACTGTCAAGCATCGCGTCTGCTAACCCGC           ACAGGTCC   AA   CGCCGGA   203           TCCGGCG   TT   GGACCTGT   204               Sulfonylurea   GCGGGTTAGCAGACGCGATGCTTGACAGTGTTCCTCTTGTCGCCA   205       Resistance   TTACAGGACAGGTCC   AG   CGCCGGATGATCGGTACTGACGCCTTC       ALS 1   CAAGAGACACCAATCGTTGAGGTAACGAGGTC         Brassica napus     GACCTCGTTACCTCAACGATTGGTGTCTCTTGGAAGGCGTCAGTA   206       Pro182Gln   CCGATCATCCGGCG   CT   GGACCTGTCCTGTAATGGCGACAAGAGG       CCT-CAG   AACACTGTCAAGCATCGCGTCTGCTAACCCGC           ACAGGTCC   AG   CGCCGGA   207           TCCGGCG   CT   GGACCTGT   208               Imidazolinone   GACCATACCTGTTGGATGTGATATGTCCGCACCAAGAACATGTGT   209       Resistance   TACCGATGATCCCAA   A   TGGTGGCACTTTCAAAGATGTAATAACAG       ALS 1   AAGGGGATGGTCGCACTAAGTACTGAGAGAT         Brassica napus     ATCTCTCAGTACTTAGTGCGACCATCCCCTTCTGTTATTACATCTTT   210       Ser638Asn   GAAAGTGCCACCA   T   TTGGGATCATCGGTAACACATGTTCTTGGTG       AGT-AAT   CGGACATATCACATCCAACAGGTATGGTC           GATCCCAA   A   TGGTGGCA   211           TGCCACCA   T   TTGGGATC   212               Sulfonylurea   CAGCGGGTTAGCAGACGCGATGCTTGACAGTGTTCCTCTTGTCGC   213       Resistance   CATTACAGGACAGGT   T   CCTCGCCGGATGATCGGTACTGACGCCTT       ALS 2   CCAAGAGACACCAATCGTTGAGGTAACGAGG         Brassica napus     CCTCGTTACCTCAACGATTGGTGTCTCTTGGAAGGCGTCAGTACC   214       Pro126Ser   GATCATCCGGCGAGG   A   ACCTGTCCTGTAATGGCGACAAGAGGAA       CCC-TCC   CACTGTCAAGCATCGCGTCTGCTAACCCGCTG           GGACAGGT   T   CCTCGCCG   215           CGGCGAGG   A   ACCTGTCC   216               Sulfonylurea   AGCGGGTTAGCAGACGCGATGCTTGACAGTGTTCCTCTTGTCGCC   217       Resistance   ATTACAGGACAGGTC   A   CTCGCCGGATGATCGGTACTGACGCCTTC       ALS 2   CAAGAGACACCAATCGTTGAGGTAACGAGGT         Brassica napus     ACCTCGTTACCTCAACGATTGGTGTCTCTTGGAAGGCGTCAGTAC   218       Pro126Gln   CGATCATCCGGCGAG   T   GACCTGTCCTGTAATGGCGACAAGAGGA       CCC-CAG   ACACTGTCAAGCATCGCGTCTGCTAACCCGCT           GACAGGTC   A   CTCGCCGG   219           CCGGCGAG   T   GACCTGTC   220               Imidazolinone   GACCATACCTGTTGGATGTGATATGTCCGCACCAAGAACATGTGT   221       Resistance   TACCGATGATCCCAA   A   TGGTGGCACTTTCAAAGATGTAATAACAG       ALS 2   AAGGGGATGGTCGCACTAAGTACTGAGAGAT         Brassica napus     ATCTCTCAGTACTTAGTGCGACCATCCCCTTCTGTTATTACATCTTT   222       Ser582Asn   GAAAGTGCCACCA   T   TTGGGATCATCGGTAACACATGTTCTTGGTG       AGT-AAT   CGGACATATCACATCCAACAGGTATGGTC           GATCCCAA   A   TGGTGGCA   223           TGCCACCA   T   TTGGGATC   224               Sulfonylurea   AGCGGGTTAGCCGACGCGATGCTTGACAGTGTTCCTCTCGTCGC   225       Resistance   CATCACAGGACAGGTC   T   CTCGCCGGATGATCGGTACTGACGCGT       ALS 3   TCCAAGAGACGCCAATCGTTGAGGTAACGAGGT         Brassica napus     ACCTCGTTACCTCAACGATTGGCGTCTCTTGGAACGCGTCAGTAC   226       Pro179Ser   CGATCATCCGGCGAG   A   GACCTGTCCTGTGATGGCGACGAGAGGA       CCT-TCT   ACACTGTCAAGCATCGCGTCGGCTAACCCGCT           GACAGGTC   T   CTCGCCGG   227           CCGGCGAG   A   GACCTGTC   228               Sulfonylurea   GCGGGTTAGCCGACGCGATGCTTGACAGTGTTCCTCTCGTCGCC   229       Resistance   ATCACAGGACAGGTCC   AA   CGCCGGATGATCGGTACTGACGCGTT       ALS 3   CCAAGAGACGCCAATCGTTGAGGTAACGAGGTC         Brassica napus     GACCTCGTTACCTCAACGATTGGCGTCTCTTGGAACGCGTCAGTA   230       Pro179Gln   CCGATCATCCGGCG   TT   GGACCTGTCCTGTGATGGCGACGAGAGG       CCT-CAA   AACACTGTCAAGCATCGCGTCGGCTAACCCGC           ACAGGTCC   AA ee CGCCGGA     231           TCCGGCG   TT   GGACCTGT   232               Sulfonylurea   GCGGGTTAGCCGACGCGATGCTTGACAGTGTTCCTCTCGTCGCC   233       Resistance   ATCACAGGACAGGTCC   AG   CGCCGGATGATCGGTACTGACGCGTT       ALS 3   CCAAGAGACGCCAATCGTTGAGGTAACGAGGTC         Brassica napus     GACCTCGTTACCTCAACGATTGGCGTCTCTTGGAACGCGTCAGTA   234       Pro179Gln   CCGATCATCCGGCG   CT   GGACCTGTCCTGTGATGGCGACGAGAGG       CCT-CAG   AACACTGTCAAGCATCGCGTCGGCTAACCCGC           ACAGGTCC   AG   CGCCGGA   235           TCCGGCG   CT   GGACCTGT   236               Imidazolinone   GACCGTACCTGTTGGATGTCATCTGTCCGCACCAAGAACATGTGT   237       Resistance   TACOGATGATCCCAA   A   TGGTGGCACTTTCAAAGATGTAATAACCG       ALS 3   AAGGGGATGGTCGCACTAAGTACTGAGAGAT         Brassica napus     ATCTCTCAGTACTTAGTGCGACCATCCCCTTCGGTTATTACATCTT   238       Ser635Asn   TGAAAGTGCCACCA   T   TTGGGATCATCGGTAACACATGTTCTTGGT       AGT-AAT   GCGGACAGATGACATCCAACAGGTACGGTC           GATCCCAA   A   TGGTGGCA   239           TGCCACCA   T   TTGGGATC   240               Sultonylurea   TCCGCGCTCGCCGACGCGCTGCTCGACTCCGTCCCGATGGTCGC   241       Resistance   CATCACGGGCCAGGTC   T   CCCGCCGCATGATCGGCACCGACGCCT       ALS   TCCAGGAGACGCCCATAGTCGAGGTCACCCGCT         Oryza sativa     AGCGGGTGACCTCGACTATGGGCGTCTCCTGGAAGGCGTCGGTG   242       Prol7l Ser   CCGATCATGCGGCGGG   A   GACCTGGCCCGTGATGGCGACCATCG       CCC-TCC   GGACGGAGTCGAGCAGCGCGTCGGCGAGCGCGGA           GCCAGGTC   T   CCCGCCGC   243           GCGGCGGG   A   GACCTGGC   244               Sulfonylurea   CCGCGCTCGCCGACGCGCTGCTCGACTCCGTCCCGATGGTCGCC   245       Resistance   ATCACGGGCCAGGTCC   AA   CGCCGCATGATCGGCACCGACGCCTT       ALS   CCAGGAGACGCCCATAGTCGAGGTCACCCGCTC         Oryza sativa     GAGCGGGTGACCTCGACTATGGGCGTCTCCTGGAAGGCGTCGGT   246       Pro171Gln   GCCGATCATGCGGCG   T ee TGGACCTGGCCCGTGATGGCGACCATCG         CCC-CAA   GGACGGAGTCGAGCAGCGCGTCGGCGAGCGCGG           CCAGGTCC   AA   CGCCGCA   247           TGCGGCG   TT   GGACCTGG   248               Sulfonylurea   CCGCGCTCGCCGACGCGCTGCTCGACTCCGTCCCGATGGTCGCC   249       Resistance   ATCACGGGCCAGGTCC   AG   CGCCGCATGATCGGCACCGACGCCTT       ALS   CCAGGAGACGCCCATAGTCGAGGTCACCCGCTC         Oryza sativa     GAGCGGGTGACCTCGACTATGGGCGTCTCCTGGAAGGCGTCGGT 250       Pro171Gln   GCCGATCATGCGGCG   CT   GGACCTGGCCCGTGATGGGGACCATCG       CCC-CAG   GGACGGAGTCGAGCAGCGCGTCGGCGAGCGCGG           CCAGGTCC   AG   CGCCGCA   251           TGCGGCG   CT   GGACCTGG   252               Imidazolinone   GGCCATACTTGTTGGATATCATCGTCCCGCACCAGGAGCATGTGC   253       Resistance   TGCCTATGATCCCAA   A   TGGGGGCGCATTCAAGGACATGATCCTGG       ALS   ATGGTGATGGCAGGACTGTGTATTAATCTAT         Oryza sativa     ATAGATTAATACACAGTCCTGCCATCACCATCCAGGATCATGTCCT   254       Ser627Asn   TGAATGCGCCCCCA   T   TTGGGATCATAGGCAGCACATGCICCTGGI       AGT-AAT   GCGGGACGATGATATCCAACAAGTATGGCC           GATCCCAA   A   TGGGGGCG   255           CGCCCCGA   T   TTGGGATC   256               Sulfonylurea   TCTGCGCTCGCAGACGCGTTGCTCGACTCCGTCCCCATGGTCGC   257       Resistance   CATCACGGGACAGGTG   T   CGCGACGCATGATTGGCACCGACGCCT       ALS   TTCAGGAGACGCCCATCGTCGAGGTCACCCGCT         Zea mays     AGCGGGTGACCTCGACGATGGGCGTCTCCTGAAAGGCGTCGGTG   258       Pro165Ser   CCAATCATGCGTCGCG   A   CACCTGTCCCGTGATGGCGACCATGGG       CCG-TCG   GACGGAGTCGAGCAACGCGTCTGCGAGCGCAGA           GACAGGTG   T   CGCGACGC   259           GCGTCGCG   A   CACCTGTC   260               Sulfonylurea   CTGCGCTCGCAGACGCGTTGCTCGACTCCGTCCCCATGGTCGCC   261       Resistance   ATCACGGGACAGGTGC   A   GCGACGCATGATTGGCACCGACGCCTT       ALS   TCAGGAGACGCCCATCGTCGAGGTCACCCGCTC         Zea mays     GAGCGGGTGACCTCGACGATGGGCGTCTCCTGAAAGGCGTCGGT 262       Pro165Gln   GCCAATCATGCGTCGC   T   GCACCTGTCCCGTGATGGCGACCATGG       CCG-CAG   GGACGGAGTCGAGCAACGCGTCTGCGAGCGCAG           ACAGGTGC   A   GCGACGCA   263           TGCGTCGC   T   GCACCTGT   264               Imidazolinone   GGCCGTACCTCTTGGATATAATCGTCCCGCACCAGGAGCATGTGT   265       Resistance   TGCCTATGATCCCTA   A   TGGTGGGGCTTTCAAGGATATGATCCTGG       ALS   ATGGTGATGGCAGGACTGTGTATTGATCCGT         Zea mays     ACGGATCAATACACAGTCCTGCCATCACCATCCAGGATCATATCC   266       Ser621Asn   TTGAAAGCCCCACCA   T   TAGGGATCATAGGCAACACATGCTCCTGG       AGT-AAT   TGCGGGACGATTATATCCAAGAGGTACGGCC           GATCCCTA   A   TGGTGGGG   267           CCCCACCA   T   TAGGGATC   268               Sulfonylurea   AGTGGTCTCGCTGATGCAATGCTCGATAGTATCCCTCTCGTGGCG   269       Resistance   ATCACTGGICAAGTC   T   CTCGTCGGATGATCGGTACCGATGCTTTC       ALS   CAGGAAACTCCAATTGTTGAGGTAACAAGGT         Gossypium hirsutum     ACCTTGTTACCTCAACAATTGGAGTTTCCTGGAAAGCATCGGTAC   270       Pro186Ser   CGATCATCCGACGAG   A   GACTTGACCAGTGATCGCCACGAGAGGG       CCT-TCT   ATACTATGGAGCATTGCATCAGCGAGACCACT           GTCAAGTCTC   T   CGTCGG   271           CCGACGAGAG   A   CTTGAC   272               Sulfonylurea   GTGGTCTCGCTGATGCAATGCTCGATAGTATCCCTCTCGTGGCGA   273       Resistance   TCACTGGTCAAGTCC   AA   CGTCGGATGATCGGTACCGATGCTTTCC       ALS   AGGAAACTCCAATTGTTGAGGTAACAAGGTC         Gossypium hirsutum     GACCTTGTTACCTTAACAATTGGAGTTTCCTGGAAAGCATCGGTA   274       Pro186Gln   CCGATCATCCGACG   TT   GGACTTGACCAGTGATCGCCACGAGAGG       CCT-CAA   GATACTATCGAGCATTGCATCAGCGAGACCAC           TCAAGTCC   AA   CGTCGGA   275           TTCCGACG   TT   GGACTTGA   276               Sulfonylurea   GTGGTCTCGCTGATGCAATGCTCGATAGTATCCCTCTCGTGCCGA   277       Resistance   TCACTGGTCAAGTCC   AG   CGTCGGATGATCGGTACCGATGCTTTCC       ALS   AGGAAACTCCAATTGTTGAGGTAACAAGGTC         Gossypium hirsutum     GACCTTGTTACCTCAACAATTGGAGTTTCCTGGAAAGCATCGGTA   278       Pro186Gln   CCGATCATCCGACG   CT   GGACTTGACCAGTGATCGCCACGAGAGG       CCT-CAG   GATACTATCGAGCATTGCATCAGCGAGACCAC           TCAAGTCC   AG   CGTCGGA   279           TCCGACG   CT   GGACTTGA   280               Imidazolinone   GACCTTACTTGTTGGATGTGATTGTCCCACATCAAGAACATGTCCT   281       Resistance   GCCTATGATCCCCA   A   TGGAGGGGCTTTCAAAGATGTGATCACAGA       ALS   GGGTGATGGAAGAACACAATATTGACCTCA         Gossypium hirsutum     TGAGGTCAATATTGTGTTCTTCCATCACCCTCTGTGATCACATCTT   282       Ser642Asn   TGAAAGCCCCTCCA   T   TGGGGATCATAGGCAGGACATGTTCTTGAT       AGT-AAT   GTGGGACAATCACATCCAACAAGTAAGGTC           GATCCCCA   A   TGGAGGGG   283           CCCCTCCA   T ee TGGGGATC     284               Sulfonylurea   TCTGGTCTTGCTGATGCACTTCTTGACTCAGTCCCTCTTGTCGCCA   285       Resistance   TTACTGGGCAAGTT   T   CCCGGCGTATGATTGGTACTGATGCTTTTCA       ALS   AGAGACTCCAATTGTTGAGGTAACTCGAT         Amaranthus powellii     ATCGAGTTACCTCAACAATTGGAGTCTCTTGAAAAGCATCAGTACC   286       Pro192Ser   AATCATACGCCGGG   A   AACTTGCCCAGTAATGGCGACAAGAGGGA       CCC-TCC   CTGAGTCAAGAAGTGCATCAGCAAGACCAGA           GGCAAGTT   T   CCCGGCGT   287           ACGCCGGG   A   AACTTGCC   288               Sulfonymurea   CTGGTCTTGCTGATGCACTTCTTGACTCAGTCCCTCTTGTCGCCAT   289       Resistance   TACTGGGC   AA   GTTCAACGGCGTATGATTGGTACTGATGCTTTTCA       ALS   AGAGACTCCAATTGTTGAGGTAACTCGATC         Amaranthus powellii     GATCGAGTTACCTCAACAATTGGAGTCTCTTGAAAAGCATCAGTAC   290       Pro192Gln   CAATCATACGCCG   TT   GAACTTGCCCAGTAATGGCGACAAGAGGGA       CCC-CAA   CTGAGTCAAGAAGTGCATCAGCAAGACCAG           GCAAGTTC   AA   CGGCGTA   291           TACGCCG   TT   GAACTTGC   292               Sulfonylurea   CTGGTCTTGCTGATGCACTTCTTGACTCAGTCCCTCTTGTCGCCAT   293       Resistance   TACTGGGCAAGTTC   AG   CGGCGTATGATTGGTACTGATGCTTTTCA       ALS   AGAGACTCCAATTGTTGAGGTAACTCGATC         Amaranthus powellii     GATCGAGTTACCTCAACAATTGGAGTCTCTTGAAAAGCATCAGTAC   294       Pro192Gln   CAATCATACGCCG   CT   GAACTTGCCCAGTAATGGCGACAAGAGGG       CCC-CAG   ACTGAGTCAAGAAGTGCATCAGCAAGACCAG           GCAAGTTC   AG   CGGCGTA   295           TACGCCG   CT   GAACTTGC   296               Imidazolinone   GACCGTATCTGCTGGATGTAATCGTACCACATCAGGAGCATGTGC   297       Resistance   TGCCTATGATCCCTA   A   CGGTGCCGCCTTCAAGGACACCATAACAG       ALS   AGGGTGATGGAAGAAGGGCTTATTAGTTGGT         Amaranthus powellii     ACCAACTAATAAGCCCTTCTTCCATCACCCTCTGTTATGGIGTCCT   298       Ser652Asn   TGAAGGCGGCACCG   T   TAGGGATCATAGGCAGCACATGCTCCTGA       AGC-AAC   TGTGGTACGATTACATCCAGCAGATACGGTG           GATCCCTA   A   CGGTGCCG   299           CGGCACCG   T   TAGGGATC   300                    
     [0121]                   TABLE 12                          Genome-Altering Oligos Conferring Porphyric Herbicide Resistance                                 Phenotype, Gene,                   Plant &amp; Targeted        SEQ ID       Alteration   Altering Oligos   NO:               Porphyric Herbicide   TCTTGCGCCCTCTTTCTGAATCTGCTGCAAATGCACTCTCAAAACT   301           Resistant   ATATTACCCACCA   A T G   GCAGCAGTATCTATCTCGTACCCGAAAGA       PPO   AGCAATCCGAACAGAATGTTTGATAGATGG         Arabidopsis thaliana     CCATCTATCAAACATTCTGTTCGGATTGCTTCTTTCGGGTACGAGA   302       Val365Met   TAGATACTGCTG   C   CA   T   TGGTGGGTAATATAGTTTTGAGAGTGCATT       GTT-ATG   TGCAGCAGATTCAGAAAGAGGGCGCAAGA           CCCACCA   A   T   G   GCAGCAG   303           CTGCTGC   C   A   T   TGGTGGG   304               Porphyric Herbicide   TATTACGTCCTCTTTCGGTTGCCGCAGCAGATGCACTTTCAAATTT   305       Resistant   CTACTAICCCCCA   A   T   G   GGAGCAGTCACAATTTCATATCCTCAAGAA       PPO   GCTATTCGTGATGAGCGTCTGGTTGATGG         Nicotiana tabacum     CCATCAACCAGACGCTCATCACGAATAGCTTCTTGAGGATATGAA   306       Val376Met   ATTGTGACTGCTCC   C   A   TT   GGGGGATAGTAGAAATTTGAAAGTGCA       GTT-ATG   TCTGCTGCGGCAACCGAAAGAGGACGTAATA           TCCCCCA   A   T   G GGAGCAG     307           CTGCTCC   C   A   T   TGGGGGA   308               Porphyric Herbicide   TGTTGCGTCCGCTTTCGTTGGGTGCAGCAGATGCATTGTCAAAAT   309       Resistant   TTTATTATCCTCCG   A   T   G   GCAGCTGTATCAATTTCATATCCAAAAGA       PPO   CGGAATTCGTGCTGACCGGCTGATTGATGG         Cichorium intybus     CCATCAATCAGCCGGTCAGCACGAATTGCGTCTTTTGGATATGAA   310       Val383Met   ATTGATACAGCTGC   C   A   T   CGGAGGATAATAAAATTTTGACAATGCAT       GTT-ATG   CTGCTGCACCCAACGAAAGCGGACGCAACA           TCCTCCG   A   T   G GCAGCTG     311           CAGCTGC   C   A   T   CGGAGGA   312               Porphyric Herbicide   TCCTTCGTCCACTTTCAGATGTCGCCGCAGAATCTCTTTCAAAATT   313       Resistant   TCATTATCCACCA   A   T   G   GCAGCTGTGTCACTTTCCTATCCTAAAGAA       PPO   GCAATTAGATCAGAGTGCTTGATTGACGG         Spinacia oleracea     CCGTCAATCAAGCACTCTGATCTAATTGCTTCTTTAGGATAGGAAA   314       Val390Met   GTGACACAGCTGC   C   A   T   TGGTGGATAATGAAATTTTGAAAGAGATT       GTT-ATG   CTGCGGCGACATCTGAAAGTGGACGAAGGA           TCCACCA   A   T   G   GCAGCTG   315           CAGCTGC   C   A   T   TGGTGGA   316               Porphyric Herbicide   TTTTGCGTCCACTTTCAAGCGATGCTGCAGATGCTCTATCAAGATT   317       Resistant   CTATTATCCACCG   A   T   G   GCTGCIGTAACTGTTTCGTATCCAAAGGAA       PPO   GCAATTAGAAAAGAATGCTTAATTGATGG         Zea mays     CGATCAATTAAGCATTCTTTTCTAATTGCTTCCTTTGGATACGAAAC   318       Val363Met   AGTTACAGCAGC   C   A   T   CGGTGGATAATAGAATCTTGATAGAGCATC       GTT-ATG   TGCAGCATCGCTTGAAAGTGGACGCAAAA           TCCACCG   A   T   G   GCTGCTG   319           CAGCAGC   C   A   T   CGGTGGA   320               Porphyric Herbicide   TCTTGCGGCCACTTTCAAGTGATGGAGCAGATGCTCTGTCAATATT   321       Resistant   CTATTATCCACCA   A   T   G   GCTGCTGTAACTGTTTCATATCCAAAAGAA       PPO   GCAATTAGAAAAGAATGCTTAATTGACGG         Oryza sativa     CCGTCAATTAAGCATTCTTTTCTAATTGCTTCTTTTGGATATGAAAC   322       Val364Met   AGTTACAGCAGC   C   A   T   TGGTGGATAATAGAATATTGACAGAGCATC       GTT-ATG   TGCTGCATCACTTGAAAGTGGCCGCAAGA           TCCACCA   A   T   G   GCTGCTG   323           CAGCAGCCA   T   TGGTGGA   324               Porphyric Herbicide   CTGGTCAAGGAGCAGGCGCCCGCCGCCGCCGAGGCCCTGGGCT   325       Resistant   CCTTCGACTACCCGCCG   A   TGGGCGCCGTGACGCTGTCGTACCCG       PPO   CTGAGCGCCGTGCGGGAGGAGCGCAAGGCCTCGG         Chlamydomonas     CCGAGGCCTTGCGCTCCTCCCGCACGGCGCTCAGCGGGTACGAC   326         reinhardtii     AGCGTCACGGCGCCCA   T   CGGCGGGTAGTCGAAGGAGCCCAGGG       Val389Met   CCTCGGCGGCGGCGGGCGCCTGCTCCTTGACCAG       GTG-ATG   ACCCGCCG   A   TGGGCGCC   327           GGCGCCCA   T   CGGGGGGT   328                    
     [0122]                   TABLE 13                          Genome-Altering Oligos Conferring Triazine Resistance                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               Triazine Resistant   AAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGAT   329           D1 Protein   TTTCCAATATGCTA   C   TTTCAACAATTCTCGTTCTTTACATTTCTTCTT         Arabidopsis thaliana     AGCGGCTTGGCCGGTAGTAGGTATTTG       Ser264Thr   CAAATACCTACTACCGGCCAAGCCGCTAAGAAGAAATGTAAAGAA   330       AGT-ACT   CGAGAATIGTTGAAA   G   TAGCATATTGGAAAATCAATCGGCCAAAAT           AACCGTGAGCAGCTACAATGTTGTAAGTTT           ATATGCTA   C   TTTCAACA   331           TGTTGAAA   G   TAGCATAT   332       Triazine Resistant   AAACTTATAACATCGTAGCCGCTCATGGTTATTTTGGCCGATTGAT   333       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTTCGTTACACTTCTTCC         Nicotiana tabacum     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAG   G CCAAGCAGCTAGGAAGAAGTGTAACGAA     334       AGT-ACT   CGAGAGtTGTIGAAA   G   TAGCATATTGGAAGATCAAtCGGCCAAAA           TAACCATGAGCGGCTACGATGTTATAAGTTT           ATATGCTA   C   TTTCAACA   335           TGTTGAAA   G   TAGCATAT   336       Triazine Resistant   AAACTTATAATATCGTAGCCGCTCATGGTTATTTTGGCCGATTGAT   337       D1Protein   CTTCCAATATGCTA   C   TTTTAACAACTCTCGCTCTTTACATTTCTTCT         Populus deltoides     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTAAGAAGAAATGTAAAGAG   338       AGT-ACT   CGAGAGTTGTTAAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCGGCTACGATATTATAAGTTT           ATATGCTA   C   TTTTAACA   339           TGTTAAAA   G   TAGCATAT   340       Triazine Resistant   AAACTTATAATATCGTAGCCGCTCATGGTTATTTTGGCCGATTGAT   341       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTTCGTTACACTTCTTCC         Petunia x hybrida     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTAGGAAGAAGTGTAACGAA   342       AGT-ACT   CGAGAGTTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCGGCTACGATATTATAAGTTT           ATATGCTA   C   TTTCAACA   343           TGTTGAAA   G   TAGCATAT   344       Triazine Resistant   AAACTTATAAIATCGTAGCTGCTCATGGTTATTTTGGCCGATTGAT   345       D1Protein   CTTCCAATATGCTA   C   TTTCAACAATTCTCGTTCTTTACATTTCTTCC         Magnolia pyramidata     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTAGGAAGAAATGTAAAGAA   346       AGT-ACT   CGAGAATTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCAGCTACGATATTATAAGTTT           ATATGCTA   C   TTTCAACA   347           TGTTGAAA   G   TAGCATAT   348       Triazine Resistant   AAACCTATAATATTGTAGCAGCTCATGGTTATTTTGGCCGATTGAT   349       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTTCTTTACATTTCTTCC         Medicago sativa     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTAGGAAGAAATGTAAAGAA   350       AGT-ACT   CGAGAGTTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAAGCATGAGCTGCTACAATATTATAGGTTT           ATATGCTA   C   TTTCAACA   351           TGTTGAAA+E,us  G TAGCATAT   1352       Triazine Resistant   AAACCTATAATATTGTAGCTGCTCATGGTTATTTGGCCGATTGAT   353       D1Protein   CTTCCAATATGCAA   C   TTTCAACAATTCTCGTTCTTTACATTTCTTCT         Glycine max     TAGCTGCTTGGCCTGTAGTAGGTATTTG       Ser264Thr   CAAATACCTACTACAGGCCAAGCAGCTAAGAAGAAATGTAAAGAA   354       AGT-ACT   CGAGAATTGTTGAAA   G   TTGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCAGCTACAATATTATAGGTTT           ATATGCAA   C   TTTCAACA   355           TGTTGAAA   G   TTGCATAT   356       Triazine Resistant   AAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGAT   357       D1Protein   CTTCCAATATGCT   AC   TTTCAACAATTCTCGTTCTTTACATTTCTTCT         Brassica napus     TAGCGGCTTGGCCGGTAGTAGGTATTTG       Gly264Thr   CAAATACCTACTACCGGCCAAGCCGCTAAGAAGAAATGTAAAGAA   358       GGT-ACT   CGAGAAITGTTGAAA   GT   AGCATATTGGAAGATCAATCGGCCAAAA           TAACCGTGAGCAGCTACAATGTTGTAAGTTT           ATATGCT   AC   TTTCAACA   359           TGTTGAAA   GT   AGCATAT   360       Triazine Resistant   AAACTTATAATATTGTGGCCGCTCATGGTTATTTTGGCCGATTAAT   361       D1Protein   CTTCCAATATGCTA   C   TTTTAACAACTCTCGTTCTTTACACTTCTTCT         Oryza sativa     TGGCTGCTTGGCCTGTAGTAGGGATTTG       Ser264Thr   CAAATCCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAA   362       AGT-ACT   CGAGAGTTGTTAAAA   G   TAGCATATTGGAAGATTAATCGGCCAAAAT           AACCATGAGCGGCCACAATATTATAAGTTT           ATATGCTA   C   TTTTAACA   363           TGTTAAAA   G   TAGCATAT   364       Triazine Resistant   AGACTTATAATATTGTGGCTGCTCACGGTTATTTTGGTCGATTAAT   365       D1Protein   CTTCCAATATGCTA   C   TTTCAACAATTCTCGTTCTTTACACTTCTTCT         Zea mays     TGGCTGCTtGGCCTGTAGTAGGGATCtG       Ser264Thr   CAGATCCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAA   366       AGT-ACT   CGAGAATTGTTGAAA   G   TAGCATATTGGAAGATTAATCGACCAAAAT           AACCGTGAGCAGCCACAATATTATAAGTCT           ATATGCTA   C   TTTCAACA   367           TGTTGAAA G TAGCATAT   368       Triazine Resistant   AAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGAT   369       D1Protein   TTTCCAATATGCTA   C   TTTCAACAATTCTCGTTCTTTACATTTCTTCTT         Arabidopsis thaliana     AGCGGCTTGGCCGGTAGTAGGTATTTG       Ser264Thr   CAAATACCTACTACCGGCCAAGCCGCTAAGAAGAAATGTAAAGAA   370       AGT-ACT   CGAGAATTGITGAAA   G   TAGCATATTGGAAAATCAATCGGCCAAAAT           AACCGTGAGCAGCTACAATGTTGTAAGTTT           ATATGCTA   C   TTTCAACA   371           TGTTGAAA   G   TAGCATAT   372       Triazine Resistant   AAACTTATAACATCGTAGCCGCTCATGGTTATTTTGGCCGATTGAT   373       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTTCGTTACACTTCTTCC         Nicotiana tabacum     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTAGGAAGAAGTGTAACGAA   374       AGT-ACT   CGAGAGTTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCGGCTACGATGTTATAAGTTT           ATATGCTA   C   TTTCAACA   375           TGTTGAAA   G TAGCATAT     376       Triazine Resistant   AAACTTATAATATCGTAGCCGCTCATGGTTATTTTGGCCGATTGAT   377       D1Protein   CTTCCAATATGCTA   C   TTTTAACAACTCTCGCTCTTTACATTTCTTCT         Papulus deltoides     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGGTAAGAAGAAATGTAAAGAG   378       AGT-AGT   CGAGAGTTGTTAAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCGGCTACGATATTATAAGTTT           ATATGCTA   C   TTTTAACA   379           TGTTAAAA   G   TAGCATAT   380       Triazine Resistant   AAACTTATAATATCGTAGCCGCTCATGGTTATTTTGGCCGATTGAT   381       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTTCGTTACACTTCTTCC         Petunia x hybrida     TAGCTGGTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTAGGAAGAAGTGTAACGAA   382       AGT-ACT   CGAGAGTTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCGGCTACGATATTATAAGTTT           ATATGCTA   C   TTTCAACA   383           TGTTGAAA   G   TAGCATAT   384       Triazine Resistant   AAACTTATAATATCGTAGCTGCTCATGGTTATTTTGGCCGATTGAT   385       D1Protein   CTTCCAATATGCTA   C   TTTCAACAATTCTCGTTCTTTACATTTCTTCC         Magnolia pyramidata     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTAGGAAGAAATGTAAAGAA   386       AGT-ACT   CGAGAATTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCAGCTACGATATTATAAGTTT           ATATGCTA   C   TTTCAACA   387           TGTTGAAA   G   TAGCATAT   388       Triazine Resistant   AAACCTATAATATTGTAGCAGCTCATGGTTATTTTGGCCGATTGAT   389       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTTCTTTACATTTGTTCC         Medicago sativa     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTAGGAAGAAATGTAAAGAA   390       AGT-ACT   CGAGAGTTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCTGCTACAATATTATAGGTTT           ATATGCTA   C   TTTCAACA   391           TGTTGAAA   G   TAGCATAT   392       Triazine Resistant   AAACCTATAATATTGTAGCTGCTCATGGTTATTTTGGCCGATTGAT   393       D1Protein   CTTCCAATATGCAA   C   TTTCAACAATTCTCGTTCTTTACATTTCTTCT         Glycine max     TAGCTGCTTGGCCTGTAGTAGGTATTTG       Ser264Thr   CAAATACCTACTACAGGCCAAGCAGCTAAGAAGAAATGTAAAGAA   394       AGT-ACT   CGAGAATTGTTGAAA   G   TTGCATATTGGAAGATCAATCGGGCAAAA           TAACCATGAGCAGCTACAATATTATAGGTTT           ATATGCAA   C   TTTCAACA   395           TGTTGAAA   G   TTGCATAT   396       Triazine Resistant   AAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGAT   397       D1Protein   CTTCCAATATGCT   AC   TTTCAACAATTCTCGTTCTTTACATTTCTTCT         Brassica napus     TAGCGGCTTGGCCGGTAGTAGGTATTTG       Gly264Thr   CAAATACCTACTACCGGCCAAGCCGCTAAGAAGAAATGTAAAGAA   398       GGT-ACT   CGAGAATTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCGTGAGCAGCTACAATGTTGTAAGTTT           ATATGCT   AC   TTTCAACA   399           TGTTGAAA   GT   AGCATAT   400       Triazine Resistant   AAACTTATAATATTGTGGCCGCTCATGGTTATTTTGGCCGATTAAT   401       D1Protein   CTTCCAATATGCTA   C   TTTTAACAACTCTCGTTCTTTACACTTCTTCT         Oryza sativa     TGGCTGCTTGGCCTGTAGTAGGGATTTG       Ser264Ihr   CAAATCCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAA   402       AGT-ACT   CGAGAGTTGTTAAAA   G   TAGCATATTGGAAGATTAATCGGCCAAAAT           AACCATGAGCGGCCACAATATTATAAGTTT           ATATGCTA   C   TTTTAACA   403           TGTTAAAA   G   TAGCATAT   404       Triazine Resistant   AGACTTATAATATTGTGGCTGCTCACGGTTATTTTGGTCGATTAAT   405       D1Protein   CTTCCAATATGCTA   C   TTTCAACAATTCTCGTTCTTTACACTTCTTCT         Zea mays     TGGCTGCTTGGCCTGTAGTAGGGATCTG       Ser264Thr   CAGATCCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAA   406       AGT-ACT   CGAGAATTGTTGAAA   G   TAGCATATTGGAAGATTAATCGACCAAAAT           AACCGTGAGCAGCCACAATATTATAAGTCT           ATATGCTA   C   TTTCAACA   407           TGTTGAAA G TAGCATAT   408       Triazine Resistant   AAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGAT   409       D1Protein   TTTCCAATATGCTA   C   TTTCAACAATTCTCGTTCTTTACATTTCTTCTT         Arabidopsis thaliana     AGCGGCTTGGCCGGTAGTAGGTATTTG       Ser264Thr   CAAATACCTACTACCGGCCAAGCCGCTAAGAAGAAATGTAAAGAA   410       AGT-ACT   CGAGAATTGTTGAAA   G   TAGCATATTGGAAAATCAATCGGCCAAAAT           AACCGTGAGCAGCTACAATGTTGTAAGTTT           ATATGCTA   C   TTTCAACA   411           TGTTGAAA   G   TAGCATAT   412       Triazine Resistant   AAACCTACAATATTGTGGCTGCTCACGGTTATTTCGGCCGATTGAT   413       D1Protein   CTTCCAGTATGCTA   C   TTTCAACAACTCCCGTTCTTTACATTTCTTCT         Picea abies     TAGCTGCTTGGCCCGTAGCAGGTATCTG       Ser264Thr   CAGATACCTGCTACGGGCCAAGCAGCTAAGAAGAAATGTAAAGAA   414       AGT-ACT   CGGGAGTTGTTGAAA   G   TAGCATACTGGAAGATCAATCGGCCGAAA           TAACCGTGAGCAGCCACAATATTGTAGGTTT           GTATGCTA   C   TTTCAACA   415           TGTTGAAA   G   TAGCATAC   416       Triazine Resistant   AAACCTATAATATTGTAGCTGCTCACGGTTATTTTGGCCGATTGAT   417       D1Protein   CTTCCAATATGCTA   C   TTTCAACAATTCTCGCTCTTTACATTTCTTCC         Vicia faba     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTAGGAAGAAATGTAAAGAG   418       AGT-ACT   CGAGAATTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCGTGAGCAGCTACAATATTATAGGTTT           ATATGCTA   C   TTTCAACA   419           TGTTGAAA   G   TAGCATAT   420       Triazine Resistant   AGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT   421       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTTCTTTACACTTCTTCT         Hordeum vulgare     TGGCTGCTTGGCCTGTAGTAGGAATCTG       Ser264Thr   CAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAA   422       AGT-ACT   CGAGAGTTGTTGAAAGTAGCATATTGGAAGATTAATCGGCCAAAA           TAACCATGAGCAGCCACAATATTATAAGTCT           ATATGCTACTTTCAACA   423           TGTTGAAAGTAGCATAT   424       Triazine Resistant   AAACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT   425       D1Protein   CTTCCAATATGCTACTTTCAACAACTCTCGTTCTTTACACTTCTTCT         Triticum aestivum     TGGCTGCTTGGCCTGTAGTAGGAATCTG       Ser264Thr   CAGATTCCTACTACAGGCCMGCAGCCAAGAAGAAGTGTAAAGAA   426       AGT-ACT   CGAGAGTTGTTGAAA   G   TAGCATATTGGAAGATTAATCGGCCAAAA           TAACCATGAGCAGCCACAATATTATAAGTTT           ATATGCTA   C   TTTCAACA   427           TGTTGAAA   G +E TAGCATAT     428       Triazine Resistant   AAACTTATAATATTGTAGCTGCTCATGGTTATTTTGGCCGATTAATC   429       D1Protein   TTCCAATATGCAA   C   TTTCMCAATTCTCGTTCTTTACATTTCTTCCT         Vigna unguiculata     AGCTGCTTGGCCTGTAGTAGGTATTTG       Ser264Thr   CAAATACCTACTACAGGCCAAGCAGCTAGGAAGAAATGTAAAGAA   430       AGT-ACT   CGAGAATTGTTGAAA   G   TTGCATATTGGAAGATTAATCGGCCAAAAT           AACCATGAGCAGCTACAATATTATAAGTTT           ATATGCAA   C   TTTCAACA   431           TGTTGAAA   G   TTGCATAT   432       Triazine Resistant   AAACCTATAATATTGTAGCTGCTCACGGTTATTTTGGCCGATTGAT   433       D1Protein   CTTCCAATATGCAA   C   TTTCAACAACTCTCGTTCTTTACACTTCTTCT         Lotus japonicus     TAGCTGCTTGGCCTGTTGTAGGTATCTG       Ser264Thr   CAGATACCTACAACAGGCCAAGCAGCTAAGAAGAAGTGTAAAGAA   434       AGT-ACT   CGAGAGTTGTTGAAA   G   TTGCATATTGGAAGATCAATCGGCCAAAA           TAACCGTGAGCAGCTACAATATTATAGGTTT           ATATGCAA   C   TTTCAACA   435           TGTTGAAA   G   TTGCATAT   436       Triazine Resistant   AAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGAT   437       D1Protein   CTTCCAATATGCTA   C   TTTCAACAATTCTCGTTCTTTACATTTCTTCT         Sinapis alba     TAGCGGCTTGGCCGGTAGTAGGTATTTG       Ser264Thr   CAAATACCTACTACCGGCCAAGCCGCTAAGAAGAAATGTAAAGAA   438       AGT-ACT   CGAGAATTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCGTGAGCAGCTACAATGTTGTAAGTTT           ATATGCTA   C   TTTCAACA   439           TGTTGAAA   G   TAGCATAT   440       Triazine Resistant   AAACCTATAATATTGTAGCTGCTCACGGTTATTTTGGCCGATTGAT   441       D1Protein   CTTCCAATATGCTA   C   TTTCAACAATTCTCGCTCTTTACATTTCTTCC         Pisum sativum     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTIAGGAAGAAATGTAAAGAG   442       AGt-ACT   CGAGAATTGTTGAAAGTAGCAtATTGGAAGATCAATCGGCCAAAA           TAACCGTGAGCAGCTACAATATTATAGGTTT           ATATGCTA   C   TTTCAACA   443           TGTTGAAA   G   TAGCATAT   444       Triazine Resistant   AAACTTATAATATCGTAGGTGCTCATGGTTATTTTGGTCGATTGAT   445       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTTCTTTACACTTCTTCT         Spinacia oleracea     TAGCTGCTTGGCCTGIAGTAGGTATTTG       Ser264Thr   CAAATACCTACTACAGGCCAAGCAGCTAAGAAGAAGTGTAAAGAA   446       AGT-ACT   CGAGAGTTGTTGAAA   G   TAGCATATTGGAAGATCAATCGACCAAAA           TAACCATGAGCAGGTACGATATTATAAGTTT           ATATGCTA   C   TTTCAACA   447           TGTTGAAA   G   TAGCATAT   448       Triazine Resistant   AAACTTATAACATCGTAGCCGCTCATGGTTATTTTGGCCGATTGAT   449       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTTCGTTACACTTCTTCC         Nicotiana debneyi     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGAtACCtACTACAGGCGAAGCAGCtAGGAAGAAGTGTAACGAA   450       AGT-ACT   CGAGAGtTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCGGCTACGATGTTATAAGTTT           ATATGCTA   C   TTTCAACA   451           TGTTGAAA   G   TAGCATAT   452       Triazine Resistant   AAACTTATAATATCGTAGCCGCTCATGGTTATTTTGGCCGATTGAT   453       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTTCGTTACACTTCTTCC         Solanum nigrum     TAGCTGCTTGGCCTGTAGTAGGTATCTG       Ser264Thr   CAGATACCTACTACAGGCCAAGCAGCTAGGAAGAAGTGTAACGAA   454       AGT-ACT   CGAGAGTTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA           TAACCATGAGCGGCTACGATATTATAAGTTT           ATATGCTA   C   TTTCAACA   455           TGTTGAAA   G   TAGCATAT   456       Triazine Resistant   AAACTTATAACATCGTAGCCGCTCATGGTTATTTTGGCCGATTGAT   457       D1Protein   CTTCCAATATGCTA   C   TTTCAACAACTCTCGTICGTTACACTTCTTCC         Nicotiana     TAGCTGCTTGGCCTGTAGTAGGTATCTG         plumbaginifolia     CAGATACCTACTACAGGCCAAGCAGCTAGGAAGAAGTGTAACGAA   458       Ser264Thr   CGAGAGTTGTTGAAA   G   TAGCATATTGGAAGATCAATCGGCCAAAA       AGT-ACT   TAACCATGAGCGGCTACGATGTTATAAGTTT           ATATGCTA   C   TTTCAACA   459           TGTTGAAA   G   TAGCATAT   460                    
     EXAMPLE 6  
     Engineering Male- or Female-Sterile Plants  
     [0123] Flower development in distantly related dicot plant species is increasingly better understood and appears to be regulated by a family of genes which encode regulatory proteins. These genes include, for example, AGAMOUS (AG), APETALA1 (AP1), and APETALA3 (AP3) and PISTILLATA (PI) in  Arabidopsis thaliana,  and DEFICIENS A (DEFA), GLOBOSA (GLO), SQUAMOSA (SQUA), and PLENA (PLE) in  Antirrhinum majus.  Genetic studies have shown that the DEFA, GLO and AP3 genes are essential for petal and stamen development. Sequence analysis of these genes revealed that the gene products contain a conserved MADS box region, a DNA-binding domain. Using these clones as probes, MADS box genes have also been isolated from other species including tomato, tobacco, petunia,  Brassica napus,  and maize.  
     [0124] Altering the expression of these genes results in altered floral morphology. For example, mutations in AP3 and PI result in male-sterile flowers because petals develop in place of stamens.  
     [0125] The attached tables disclose exemplary oligonucleotide base sequences which can be used to generate site-specific mutations that confer altered floral structures in plants.  
                   TABLE 14                          Oligonucleotides to produce male-sterile plants                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               Male-sterile   TTGTCCTCTCCACCAAATCTCTTCAACAAAAAGATTAAACAAAGAG   461           AP3   AGAAGAATATGGCG   T   GAGGGAAGATCCAGATCAAGAGGATAGAGA             Arabidopsis thaliana     ACCAGACAAACAGACAAGTGACGTATTCAA           Arg3Term   TTGAATACGTCACTTGTCTGTTTGTCTGGTTCTCTATCCTCTTGATC   462       AGA-TGA   TGGATCTTCCCTC   A   CGCCATATTCTTCTCTCTTIGTTTAATCTTTTT               GTTGAAGAGATTTGGTGGAGAGGACAA               ATATGGCG   T   GAGGGAAG   463           CTTCCCTC   A   CGCCATAT   464               Male-sterile   TCTCCACCAAATCTCTTCAACAAAAAGATTAAACAAAGAGAGAAGA   465       AP3   ATATGGCGAGAGGG   T   AGATCCAGATCAAGAGGATAGAGAACCAGA             Arabidopsis thaliana     CAAACAGACAAGTGACGTATTCAAAGAGAA           Lys5Term   TTCTCTTTGAATACGTCACTTGTCTGTTTGTCTGGTTCTCTATCCTC   466       AAG-TAG   TTGATCTGGATCT   A   CCCTCTCGCCATATTCTTCTCTCTTTGTTTAAT               CTTTTTGTTGAAGAGATTTGGTGGAGA               CGAGAGGG   T   AGATCCAG   467           CTGGATCT   A   CCGTCTCG   468               Male-sterile   CCAAATCTCTTCAACAAAAAGATTAAACAAAGAGAGAAGAATATGG   469       AP3   CGAGAGGGAAGATC   T   AGATCAAGAGGATAGAGAAGCAGACAAACA             Arabidopsis thaliana     GACAAGTGACGTATTCAAAGAGAAGGAATG           Gln7Term   CATTCCTTCTCTTTGAATACGTCACTTGTCTGTTTGTCTGGTTCTCT   470       CAG-TAG   ATCCTCTTGATCT   A   GATCTTCCCTCTCGCCATATTCTTCTCTCTTTG               TTTAATCTTTTTGTTGAAGAGATTTGG               GGAAGATC   T   AGATCAAG   471           CTTGATCT   A   GATCTTCC   472               Male-sterile   CTCTTCAACAAAAAGATTAAACAAAGAGAGAAGAATATGGCGAGAG   473       AP3   GGAAGATCCAGATC   T   AGAGGATAGAGAACCAGACAAACAGAGAAG             Arabidopsis thaliana     TGACGTATTCAAAGAGAAGGAATGGTTTAT           Lys9Term   ATAAACCATTCGTTCTCTTTGAATACGTCACTTGTCTGTTTGTCTGG   474       AAG-TAG   TTCTCTATCCTCT   A   GATCTGGATCTTCCCTCTCGCCATATTCTTCTC               TCTTTGTTTAATCTTTTTGTTGAAGAG               TCCAGATC   T   AGAGGATA   475           TATCCTCT   A   GATCTGGA   476               Male-sterile   AGAGGGAAGATCGAGATGAAGAGGATAGAGAACGAGAGGAACCG   477       AP3   ACAAGTGACGTATTCT   T   AGAGAAGAAATGGTTTGTTCAAGAAAGCT             Brassica oleracea     CACGAGCTTACAGTTTTATGTGATGCTAGGG           Lys23Term   CCCTAGCATCACATAAAACTGTAAGCTCGTGAGCTTTCTTGAACAA   478       AAG-TAG   ACCATTTCTTCTCT   A   AGAATACGTCACTTGTCGGTTGGTCTGGTTC               TCTATCCTCTTGATCTGGATCTTCCCTCT               CGTATTCT   T   AGAGAAGA   479           TCTTCTCT   A   AGAATACG   480               Male-sterile   GGGAAGATCCAGATCAAGAGGATAGAGAACCAGACCAACCGACAA   481       AP3   GTGACGTATTCTAAG   T   GAAGAAATGGTTTGTTCAAGAAAGCTCACG             Brassica oleracea     AGCTTACAGTTTTATGTGATGCTAGGGTTT           Arg24Term   AAACCCTAGCATCACATAAAACTGTAAGCTCGTGAGCTTTCTTGAA   482       AGA-TGA   CAAACCATTTCTTC   A   CTTAGAATACGTCACTTGTGGGTTGGTCTGG               TTCTCTATCCTCTTGATCTGGATCTTCCC               ATTCTAAG   T   GAAGAAAT   483           ATTTCTTC   A   CTTAGAAT   484               Male-sterile   AAGATCCAGATCAAGAGGATAGAGAACCAGACCAACCGACAAGTG   485       AP3   ACGTATTCTAAGAGA   T   GAAATGGTTTGTTCAAGAAAGCTCACGAGC             Brassica oleracea     TTACAGTTTTATGTGATGCTAGGGTTTCGA           Arg25Term   TCGAAACCCTAGCATCACATAAAACTGTAAGCTCGTGAGCTTTCTT   486       AGA-TGA   GAACAAACCATTTC   A   TCTCTTAGAATACGTCACTTGTCGGTTGGTC               TGGTTCTCTATGCTCTTGATCTGGATCTT               CTAAGAGA   T   GAAATGGT   487           ACCATTTC   A   TCTCTTAG   488               Male-sterile   TCAAGAGGATAGAGAACCAGACCAACCGACAAGTGACGTATTCTA   489       AP3   AGAGAAGAAATGGTT   A   GTTCAAGAAAGCTCACGAGCTTACAGTTTT             Brassica oleracea     ATGTGATGCTAGGGTTTCGATTATCATGTT           Leu28Term   AACATGATAATCGAAACCCTAGCATCACATAAAACTGTAAGCTCGT   490       TTG-TAG   GAGCTTTCTTGAAC   T   AACCATTTCTTCTCTTAGAATACGTCACTTGT               CGGTTGGTCTGGTTCTCTATCCTCTTGA               AAATGGTT   A   GTTCAAGA   491           TCTTGAAC   T   AACCATTT   492               Male-sterile   GGCTCGAGGGAAGATCCAGATTAAGAGGATAGAGAACCAAACAAA   493       AP3   CAGGCAGGTCACCTA   G   TCCAAGAGAAGAAATGGTTTGTTCAAGAA             Brassica napus     AGCACACGAGCTCTCTGTTCTCTGTGATGCT           Tyr21Term   AGCATCACAGAGAACAGAGAGCTCGTGTGCTTTCTTGAACAAACC   494       TAC-TAG   ATTTCTTCTCTTGGA   C   TAGGTGACCTGCCTGTTTGTTTGGTTCTCTA               TCCTCTTAATCTGGATCTTCCCTCGAGCC               GTCACCTA   G   TCCAAGAG   495           CTCTTGGA   C   TAGGTGAC   496               Male-sterile   CGAGGGAAGATCCAGATTAAGAGGATAGAGAACCAAACAAACAGG   497       AP3   CAGGTCACCTACTCC   T   AGAGAAGAAATGGTTTGTTCAAGAAAGCAC             Brassica napus     ACGAGCTCTCTGTTCTCTGTGATGCTAAAG           Lys23Term   CTTTAGCATCACAGAGAACAGAGAGCTCGTGTGCTTTCTTGAACAA   498       AAG-TAG   ACCATTTCTTCTCT   A   GGAGTAGGTGACCTGCCTGTTTGTTTGGTTC               TCTATCCTCTTAATCTGGATCTTCCCTCG               CCTACTCC   T   AGAGAAGA   499           TCTTCTCT   A   GGAGTAGG   500               Male-sterile   GGGAAGATCCAGATTAAGAGGATAGAGAACCAAACAAACAGGCAG   501       AP3   GTCACCTACTCCAAG   T   GAAGAAATGGTTTGTTCAAGAAAGCACACG             Brassica napus     AGCTCTCTGTTCTCTGTGATGCTAAAGTTT           Arg24Term   AAACTTTAGCATCACAGAGAACAGAGAGCTCGTGTGCTTTCTTGAA   502       AGA-TGA   CAAACCATTTGTTC   A   CTTGGAGTAGGTGACCTGCCTGTTTGTTTGG               TTCTCTATCCTCTTAATCTGGATCTTCCC               ACTCCAAG   T   GAAGAAAT   503           ATTTCTTC   A   CTTGGAGT   504               Male-sterile   AAGATCCAGATTAAGAGGATAGAGAACCAAACAAACAGGCAGGTC   505       AP3   ACCTACTCCAAGAGA   T   GAAATGGTTTGTTCAAGAAAGCACACGAG             Brassica napus     CTCTCTGTTCTCTGTGATGCTAAAGTTTCCA           Arg25Term   TGGAAACTTTAGCATCACAGAGAACAGAGAGCTCGTGTGCTTTCTT   506       AGA-TGA   GAACAAACCATTTC   A   TCTCTTGGAGTAGGTGACCTGCCTGTTTGTT               TGGTTCTCTATCCTCTTAATCTGGATCTT               CCAAGAGA   T   GAAATGGT   507           ACCATTTC   A   TCTCTTGG   508               Male-sterile   GGAGAGAAAGGAAAGCTGGAAGAAGAAAACAAGAGCAGTAGTGG   509       DEFA   TAGTGGTTCGATGGCT   T   GAGGGAAGATCCAGATTAAGAGGATAGA             Antirrhinum majus     GAACCAAACAAACAGGCAGGTCACCTACTCCA           Arg3Term   TGGAGTAGGTGACCTGCCTGTTTGTTTGGTTCTCTATCCTCTTAAT   510       CGA-TGA   CTGGATCTTCCCTC   A   AGCCATCGAACCACTACCACTACTGCTCTTG               TTTTCTTCTTCCAGCTTTCCTTTCTCTCC               CGATGGCT   T   GAGGGAAG   511           CTTCCCTC   A   AGCCATCG   512               Male-sterile   AAAGGAAAGCTGGAAGAAGAAAACAAGAGCAGTAGTGGTAGTGGT   513       DEFA   TCCATGGCTCGAGGG   T   AGATCCAGATTAAGAGGATAGAGAACCAA             Antirrhinum majus     ACAAACAGGCAGGTCACCTACTCCAAGAGAA           Lys5Term   TTCTCTTGGAGTAGGTGACCTGCCTGTTTGTTTGGTTCTCTATCCT   514       AAG-TAG   CTTAATCTGGATCT   A   CCCTCGAGCCATCGAACCACTAGCACTACTG               CTCTTGTTTTCTTCTTCCAGCTTTCCTTT               CTCGAGGG   T   AGATCCAG   515           CTGGATCT   A   CCCTCGAG   516               Male-sterile   AAGCTGGAAGAAGAAAACAAGAGCAGTAGTGGTAGTGGTTCGATG   517       DEFA   GCTCGAGGGAAGATC   T   AGATTAAGAGGATAGAGAACCAAACAAAC             Antirrhinum majus     AGGCAGGTCACCTACTCCAAGAGAAGAAATG           Gln7Term   CATTTCTTCTCTTGGAGTAGGTGACCTGCCTGTTTGTTTGGTTCTC   518       CAG-TAG   TATCCTCTTAATCT   A   GATCTTCCCTCGAGCCATCGAACCACTACCA           CTACTGCTCTTGTTTTCTTCTTCCAGCTT               GGAAGATC   T   AGATTAAG   519           CTTAATCT   A   GATCTTCC   520               Male-sterile   GAAGAAGAAAACAAGAGCAGTAGTGGTAGTGGTTCGATGGCTCGA   521       DEFA   GGGAAGATCCAGATT   T   AGAGGATAGAGAACCAAACAAACAGGCAG             Antirrhinum majus     GTCACCTACTCCAAGAGAAGAAATGGTTTGT           Lys9Term   ACAAACCATTTCTTCTCTTGGAGTAGGTGACCTGCCTGTTTGTTTG   522       AAG-TAG   GTTCTCTATCCTCT   A   AATCTGGATCTTCCCTCGAGCCATCGAACCA               CTACCACTACTGCTCTTGTTTTCTTCTTC               TCCAGATT   T   AGAGGATA   523           TATCCTCT   A   AATCTGGA   524               Male-sterile   TCAGTAATTCTTAAGATCTCAAACTTTGAGCAAAAAGAAAAAAAAAC   525       AP3   TATGGCTCGTGGG   T   AGATCCAGATCAAGAGAATAGAGAACCAAAC             Nicotiana tabacum     AAACAGACAAGTCACTTATTCTAAGAGAA           Lys5Term   TTCTCTTAGAATAAGTGACTTGTCTGTTTGTTTGGTTCTCTATTCTC   526       AAG-TAG   TTGATCTGGATCT   A   CCCACGAGCCATAGTTTTTTTTTCTTTTTGCTC               AAAGTTTGAGATCTTAAGAATTACTGA               CTCGTGGG   T   AGATCCAG   527           CTGGATCT   A   CCCACGAG   528               Male-sterile   ATTCTTAAGATCTCAAACTTTGAGCAAAAAGAAAAAAAAACTATGGC   529       AP3   TCGTGGGAAGATC   T   AGATCAAGAGAATAGAGAACCAAACAAACAG             Nicotiana tabacum     ACAAGTCACTTATTCTAAGAGAAGAAATG           Gln7Term   CATTTCTTCTCTTAGAATAAGTGACTTGTCTGTTTGTTTGGTTCTCT   530       CAG-TAG   ATTCTCTTGATCT   A   GATCTTCCCACGAGCCATAGTTTTTTTTTCTTT               TTGCTCAAAGTTTGAGATCTTAAGAAT               GGAAGATC   T   AGATCAAG   531           CTTGATCT   A   GATCTTCC   532               Male-sterile   AAGATCTCAAACTTTGAGCAAAAAGAAAAAAAAACTATGGCTCGTG   533       AP3   GGAAGATCCAGATC   T   AGAGAATAGAGAACCAAACAAACAGACAAG             Nicotiana tabacum     TCACTTATTCTAAGAGAAGAAATGGACTTT           Lys9Term   AAAGTCCATTTCTTCTCTTAGAATAAGTGACTTGTCTGTTTGTTTGG   534       AAG-TAG   TTCTCTATTCTCT   A   GATCTGGATCTTCCCACGAGCCATAGTTTTTTT               TTCTTTTTGCTCAAAGTTTGAGATCTT               TCCAGATC   T   AGAGAATA   535           TATTCTCT+E,un  A GATCTGGA   536               Male-sterile   ATCTCAAACTTTGAGCAAAAAGAAAAAAAAACTATGGCTCGTGGGA   537       AP3   AGATCCAGATCAAG   T   GAATAGAGAACCAAACAAACAGACAAGTCA             Nicotiana tabacum     CTTATTCTAAGAGAAGAAATGGACTTTTCA           Arg10Term   TGAAAAGTCCATTTCTTCTCTTAGAATAAGTGACTTGTCTGTTTGTT   538       AGA-TGA   TGGTTCTCTATTC   A   CTTGATCTGGATCTTCCCACGAGCCATAGTTT               TTTTTTCTTTTTGCTCAAAGTTTGAGAT               AGATCAAG   T   GAATAGAG   539           CTCTATTC   A   CTTGATCT   540               Male-sterile   GGCTCGAGGAAAGATCCAGATCAAGAGAATAGAGAACACAACGAA   541       AP3   CAGACAAGTAACTTA   G   TCAAAACGAAGGGATGGTCTTTTCAAGAAG             Medicago sativa     GCCAATGAGCTCACTGTTCTTTGTGATGCT           Tyr21Term   AGCATCACAAAGAACAGTGAGCTCATTGGCCTTCTTGAAAAGACCA   542       TAC-TAG   TCCCTTCGTTTTGA   C   TAAGTTACTTGTCTGTTCGTTGTGTTCTCTAT               TCTCTTGATCTGGATCTTTCCTCGAGCC               GTAACTTA   G   TCAAAACG   543           CGTTTTGA   C   TAAGTTAC   544               Male-sterile   CTCGAGGAAAGATCCAGATCAAGAGAATAGAGAACACAACGAACA   545       AP3   GACAAGTAACTTACT   G   AAAACGAAGGGATGGTCTTTTCAAGAAGG             Medicago sativa     CCAATGAGCTCACTGTTCTTTGTGATGCTAA           Ser22Term   TTAGCATCACAAAGAACAGTGAGCTCATTGGCCTTCTTGAAAAGAC   546       TCA-TGA   CATCCCTTCGTTTT   C   AGTAAGTTACTTGTCTGTTCGTTGTGTTCTCT               ATTCTCTTGATCTGGATCTTTCCTCGAG               AACTTACT   G   AAAACGAA   547           TTCGTTTT+E,un  C AGTAAGTT   548               Male-sterile   CGAGGAAAGATCCAGATCAAGAGAATAGAGAACACAACGAACAGA   549       AP3   CAAGTAACTTACTCA   T   AACGAAGGGATGGTCTTTTCAAGAAGGCCA             Medicago sativa     ATGAGCTCACTGTTCTTTGTGATGCTAAGG           Lys23Term   CCTTAGCATCACAAAGAACAGTGAGCTCATTGGCCTTCTTGAAAAG   550       AAA-TAA   ACCATCCCTTCGTT   A   TGAGTAAGTTACTTGTCTGTTCGTTGTGTTCT               CTATTCTCTTGATCTGGATCTTTCCTCG               CTTACTCA   T   AACGAAGG   551           CCTTCGTT   A   TGAGTAAG   552               Male-sterile   GGAAAGATCCAGATCAAGAGAATAGAGAACACAACGAACAGACAA   553       AP3   GTAACTTACTCAAAA   T   GAAGGGATGGTCTTTTCAAGAAGGCCAATG             Medicago sativa     AGCTCACTGTTCTTTGTGATGCTAAGGTTT           Arg24Term   AAACCTTAGCATCACAAAGAACAGTGAGCTCATTGGCCTTCTTGAA   554       CGA-TGA   AAGACCATCCCTTC   A   TTTTGAGTAAGTTACTTGTCTGTTCGTTGTGT               TCTCTATTCTCTTGATCTGGATCTTTCC               ACTCAAAA   T   GAAGGGAT   555           ATCCCTTC   A   TTTTGAGT   556               Male-sterile   GGCTCGTGGTAAGATCCAGATCAAGAAAATAGAAAACCAAACAAAT   557       DEF4   AGGCAAGTGACTTA   G   TCAAAGAGAAGAAATGGGCTATTCAAGAAG             Solanum tuberosum     GCTAATGAACTTACAGTTCTTTGTGATGCT           Tyr21Term   AGCATCACAAAGAACTGTAAGTTCATTAGCCTTCTTGAATAGCCCA   558       TAT-TAG   TTTCTTCTCTTTGA   C   TAAGTCACTTGCCTATTTGTTTGGTTTTCTATT               TTCTTGATCTGGATCTTACCACGAGCC               GTGACTTA   G   TCAAAGAG   559           CTCTTTGA   C   TAAGTCAC   560               Male-sterile   CTCGTGGTAAGATCCAGATCAAGAAAATAGAAAACCAAACAAATAG   561       DEF4   GCAAGTGACTTATT   G   AAAGAGAAGAAATGGGCTATTCAAGAAGGC             Solanum tuberosum     TAATGAACTTACAGTTCTTTGTGATGCTAA           Ser22Term   TTAGCATCACAAAGAACTGTAAGTTCATTAGCCTTCTTGAATAGCC   562       TCA-TGA   CATTTCTTCTCTTT   C   AATAAGTCACTTGCCTATTTGTTTGGTTTTCTA               TTTTCTTGATCTGGATCTTACCACGAG               GACTTATT   G   AAAGAGAA   563           TTCTCTTT   C   AATAAGTC   564               Male-sterile   CGTGGTAAGATCCAGATCAAGAAAATAGAAAACCAAACAAATAGG   565       DEF4   CAAGTGACTTATTCA   T   AGAGAAGAAATGGGCTATTCAAGAAGGCTA             Solanum tuberosum     ATGAACTTACAGTTCTTTGTGATGCTAAAG           Lys23Term   CTTTAGCATCACAAAGAACTGTAAGTTCATTAGCCTTCTTGAATAG   566       AAG-TAG   CCCATTTCTTCTCT   A   TGAATAAGTCACTTGCCTATTTGTTTGGTTTT               CTATTTTCTTGATCTGGATCTTACCACG               CTTATTCA   T   AGAGAAGA   567           TCTTCTCT   A   TGAATAAG   568               Male-sterile   GGTAAGATCCAGATCAAGAAAATAGAAAACCAAACAAATAGGCAA   569       DEF4   GTGACTTATTCAAAG   T   GAAGAAATGGGCTATTCAAGAAGGCTAATG             Solanum tuberosum     AACTTACAGTTCTTTGTGATGCTAAAGTTT           Arg24Term   AAACTTTAGCATCACAAAGAACTGTAAGTTCATTAGCCTTCTTGAAT   570       AGA-TGA   AGCCCATTTCTTC   A   CTTTGAATAAGTCACTTGCCTATTTGTTTGGTT               TTCTATTTTCTTGATCTGGATCTTACC               ATTCAAAG   T   GAAGAAAT   571           ATTTCTTC   A   GTTTGAAT   572               Male-sterile   GCTAATGAACTTACTGTTCTTTGTGATGCTAAAGTTTCAATTGTTAT   573       AP3   GATTTCTAGTACT   T   GAAAACTTCATGAGTTTATAAGTCCCTCTATCA             Lycopersicon     CGACCAAACAATTGTTCGATCTGTACC             esculentum     GGTACAGATCGAACAATTGTTTGGTCGTGATAGAGGGACTTATAAA   574       Gly27Term   CTCATGAAGTTTTC   A   AGTACTAGAAATCATAACAATTGAAACTTTAG           GGA-TGA   CATCACAAAGAACAGTAAGTTCATTAGC               CTAGTACT   T   GAAAACTT   575           AAGTTTTC   A   AGTACTAG   576               Male-sterile   AATGAACTTACTGTTCTTTGTGATGCTAAAGTTTCAATTGTTATGAT   577       AP3   TTCTAGTACTGGA   T   AACTTCATGAGTTTATAAGTCCCTCTATCACGA             Lycopersicon     CCAAACAATTGTTCGATCTGTACCAGA             esculentum     TCTGGTACAGATCGAACAATTGTTTGGTCGTGATAGAGGGACTTAT   578       Lys28Term   AAACTCATGAAGTT   A   TCCAGTACTAGAAATCATAACAATTGAAACTT       AAA-TAA   TAGCATCACAAAGAACAGTAAGTTCATT               GTACTGGA   T   AACTTCAT   579           ATGAAGTT   A   TCCAGTAC   580               Male-sterile   ACTGTTCTTTGTGATGCTAAAGTTTCAATTGTTATGATTTCTAGTAC   581       AP3   TGGAAAACTTCAT   T   AGTTTATAAGTCCCTCTATCACGACCAAACAAT         Lycopersicon     TGTTCGATCTGTACCAGAAGACTATTG             esculentum     CAATAGTCTTCTGGTACAGATCGAACAATTGTTTGGTCGTGATAGA   582       Glu31Term   GGGACTTATAAACT   A   ATGAAGTTTTCCAGTACTAGAAATCATAACA           GAG-TAG   ATTGAAACTTTAGCATCACAAAGAACAGT               AACTTCAT   T   AGTTTATA   583           TATAAACT   A   ATGAAGTT   584               Male-sterile   ATTGTTATGATTTCTAGTACTGGAAAACTTCATGAGTTTATAAGTCC   585       AP3   CTCTATCACGACC   T   AACAATTGTTCGATCTGTACCAGAAGACTATT         Lycopersicon     GGAGTTGATATTTGGACTACTCACTATG             esculentum     CATAGTGAGTAGTCCAAATATCAACTCCAATAGTCTTCTGGTACAG   586       Lys40Term   ATCGAACAATTGTT   A   GGTCGTGATAGAGGGACTTATAAACTCATGA           AAA-TAA   AGTTTTCCAGTACTAGAAATCATAACAAT               TCACGACC   T   AACAATTG   587           CAATTGTT   A   GGTCGTGA   588               Male-sterile   GGGGCGGGGGAAGATTGAGATAAAGCGGATCGAGAACGCCACCA   589       AP3   ACAGGCAGGTGACCTA   G   TCCAAGCGCCGGTCGGGGATCATGAAG             Triticum aestivum     AAGGCGCGGGAGCTCACCGTGCTCTGCGACGCC           Tyr21Term   GGCGTCGCAGAGCACGGTGAGCTCCCGCGCCTTCTTCATGATCC   590       TAC-TAG   CCGACCGGCGCTTGGA   C   TAGGTCACCTGCCTGTTGGTGGCGTTCT           CGATCCGCTTTATCTCAATCTTCCCCCGCCCC               GTGACCTA   G   TCCAAGCG   591           CGCTTGGA   C   TAGGTCAC   592               Male-sterile   CGGGGGAAGATTGAGATAAAGCGGATCGAGAACGCCACCAACAG   593       AP3   GCAGGTGACCTACTCC   T   AGCGCCGGTCGGGGATCATGAAGAAGG         Triticum aestivum     CGCGGGAGCTCACCGTGCTCTGCGACGCCCAGG           Lys23Term   CCTGGGCGTCGCAGAGCACGGTGAGCTCCCGCGCCTTCTTCATG   594       AAG-TAG   ATCCCCGACCGGCGCT   A   GGAGTAGGTCACCTGCCTGTTGGTGGC           GTTCTCGATCCGCTTTATCTCAATCTTCCCCCG               CCTACTCC   T   AGCGCCGG   595           CCGGCGCT   A   GGAGTAGG   596               Male-sterile   TTGAGATAAAGCGGATCGAGAACGCCACCAACAGGCAGGTGACCT   597       AP3   ACTCGAAGCGCCGGT   A   GGGGATCATGAAGAAGGCGCGGGAGCTC         Triticum aestivum     ACCGTGCTCTGCGACGCCCAGGTCGCCATCAT           Ser26Term   ATGATGGCGACCTGGGCGTCGCAGAGCACGGTGAGCTCCCGCGC   598       TCG-TAG   CTTCTTCATGATCCCC   T   ACCGGCGCTTGGAGTAGGTCACCTGCCT           GTTGGTGGCGTTGTCGATCCGCTTTATCTCAA               GCGCCGGT   A   GGGGATCA   599           TGATCCCC   T   ACCGGCGC   600               Male-sterile   CGGATCGAGAACGCCACCAACAGGCAGGTGACCTACTCCAAGCG   601       AP3   CCGGTCGGGGATCATG   T   AGAAGGCGCGGGAGCTCACCGTGCTCT         Triticum aestivum     GCGACGCCCAGGTCGCCATCATCATGTTCTCCT           Lys30Term   AGGAGAACATGATGATGGCGACCTGGGCGTCGCAGAGCACGGTG   602       AAG-TAG   AGCTCCCGCGCCTTCT   A   CATGATCCCCGACCGGCGCTTGGAGTAG           GTCACCTGCCTGTTGGTGGCGTTGTCGATCCG               GGATCATG   T   AGAAGGCG   603           CGCCTTCT   A   CATGATCC   604               Male-sterile   GGGGCGCGGCAAGATCGAGATCAAGCGGATCGAGAACGCCACCA   605       Silky1   ACCGCCAGGTGACCTA   G   TCCAAGCGCCGGACGGGGATCATGAAG         Zea mays     AAGGCACGCGAGCTCACCGTGCTCTGCGACGCC           Tyr21Term   GGCGTCGCAGAGCACGGTGAGCTCGCGTGCCTTCTTCATGATCCC   606       TAG-TAG   CGTCCGGCGCTTGGA   C   TAGGTCACCTGGCGGTTGGTGGCGTTCT           CGATCGGCTTGATCTCGATCTTGCCGCGCCCC               GTGACCTA   G   TCCAAGCG   607           CGCTTGGA   C   TAGGTCAC   608               Male-sterile   CGCGGCAAGATCGAGATCAAGCGGATCGAGAACGCCACCAACCG   609       Silky1   CCAGGTGACCTACTCC   T   AGCGCCGGACGGGGATCATGAAGAAGG             Zea mays     CACGCGAGCTCACCGTGCTCTGCGACGCCCAGG           Lys23Term   CCTGGGCGTCGCAGAGCACGGTGAGCTCGCGTGCCTTCTTCATG   610       AAG-TAG   ATCCCCGTCCGGCGCT   A   GGAGTAGGTCACCTGGCGGTTGGTGGC               GTTCTCGATCCGCTTGATCTCGATCTTGCCGCG               CCTACTCC   T   AGCGCCGG   611           CCGGCGCT   A   GGAGTAGG   612               Male-sterile   CGGATCGAGAACGCCACCAACCGCCAGGTGACCTACTCCAAGCG   613       Silky1   CCGGACGGGGATCATG   T   AGAAGGCACGCGAGCTCACCGTGCTCT         Zea mays     GCGACGCCCAGGTCGCCATCATCATGTTCTCCT           Lys30Term   AGGAGAACATGATGATGGCGACCTGGGCGTCGCAGAGCACGGTG   614       AAG-TAG   AGCTCGCGTGCCTTCT   A   CATGATCCCGGTCCGGCGCTTGGAGTAG           GTCACCTGGCGGTTGGTGGCGTTCTCGATCCG               GGATCATG   T   AGAAGGCA   615           TGCCTTCT   A   CATGATCC   616               Male-sterile   ATCGAGAACGCCACCAACCGCCAGGTGACGTACTCCAAGCGCCG   617       Silky1   GACGGGGATCATGAAG   T   AGGCACGCGAGCTCACCGTGCTCTGCG         Zea mays     ACGCCCAGGTCGCCATCATCATGTTCTCCTCCA           Lys31Term   TGGAGGAGAACATGATGATGGCGACCTGGGCGTCGCAGAGCACG   618       AAG-TAG   GTGAGCTCGCGTGCCT   A   CTTCATGATCCCCGTCCGGCGCTTGGAG           TAGGTCACCTGGCGGTTGGTGGCGTTCTCGAT               TCATGAAG   T   AGGCACGC   619           GCGTGCCT   A   CTTCATGA   620               Male-sterile   GCTAGCTGCATTGTCCGGCGAGAGAGATAGCTGCTGCAGGGGGC   621       AP3   GGCCATGGGGAGGGGC   T   AGATCGAGATCAAGCGGATCGAGAACG         Oryza sativa     CGACCAACAGGCAGGTGACCTACTCGAAGCGCC           Lys5Term   GGCGCTTGGAGTAGGTCACCTGCCTGTTGGTCGCGTTCTCGATCC   622       AAG-TAG   GCTTGATCTCGATCT   A   GCCCGTCCCCATGGCGGCCCCCTGCAGCA               GCTATCTCTCTCGCCGGACAATGCAGCTAGC               GGAGGGGC   T   AGATCGAG   623           CTCGATCT   A   GCCCCTCC   624               Male-sterile   TGCATTGTCCGGCGAGAGAGATAGCTGCTGCAGGGGGCGGCCAT   625       AP3   GGGGAGGGGCAAGATC   T   AGATCAAGCGGATCGAGAACGCGACCA         Oryza sativa     ACAGGCAGGTGACCTACTCGAAGCGCCGCACGG           Glu7Term   CCGTGCGGCGCTTCGAGTAGGTCACCTGCCTGTTGGTCGCGTTCT   626       GAG-TAG   CGATCCGCTTGATCT   A   GATCTTGCCCCTCCCCATGGCCGCCCCCT               GCAGCAGCTATCTCTCTCGCCGGACAATGCA               GCAAGATC   T   AGATCAAG   627           CTTGATCT   A   GATCTTGC   628               Male-sterile   GTCCGGCGAGAGAGATAGCTGCTGCAGGGGGCGGCCATGGGGA   629       AP3   GGGGCAAGATCGAGATC   T   AGCGGATCGAGAACGCGACCAACAGG             Oryza sativa     CAGGTGACCTACTCGAAGCGCCGCACGGGGATCA           Lys9Term   TGATCCCCGTGCGGCGCTTCGAGTAGGTCACCTGCCTGTTGGTCG   630       AAG-TAG   CGTTCTCGATCCGCT   A   GATCTCGATCTTGCCCCTCCCCATGGCCG               CCCCCTGCAGCAGCTATCTCTCTCGCCGGAC               TCGAGATC   T   AGCGGATC   631           GATCCGCT   A   GATCTCGA   632               Male-sterile   GAGAGATAGCTGCTGCAGGGGGCGGCCATGGGGAGGGGCAAGA   633       AP3   TCGAGATCAAGCGGATCT   A   GAACGCGACCAACAGGCAGGTGACCT             Oryza sativa     ACTCGAAGCGCCGCACGGGGATCATGAAGAAGG           Glu12Term   CCTTCTTCATGATCCCCGTGCGGCGCTTCGAGTAGGTCACCTGCC   634       GAG-TAG   TGTTGGTCGCGTTCT   A   GATCCGCTTGATCTCGATCTTGCCCCTCCC               CATGGCGGCCCCCTGCAGCAGCTATCTCTC               AGCGGATC   T   AGAACGCG   635           CGCGTTCT   A   GATCCGCT   636                  
 
     [0126]                   TABLE 15                          Oligonucleotides to produce male-sterile plants                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               Male-sterile   TCTGTACTAATCAAATTTTGCCCTAAACGTTTTTGGCTTTGGAGCA   637           AG   GCAATCACGGCGTA   G   CAATCGGAGCTAGGAGGAGATTCCTCTCC             Arabidopsis thaliana     CTTGAGGAAATCTGGGAGAGGAAAGATCGAA           Tyr35Term   TTCGATCTTTCCTCTCCCAGATTTCCTCAAGGGAGAGGAATCTCCT   638       TAG-TAG   CCTAGGTCCGATTG   C   TACGCCGTGATTGCTGCTCCAAAGCCAAAA               ACGTTTAGGGCAAAATTTGATTAGTACAGA               ACGGCGTA   G   CAATCGGA   639           TCCGATTG   C   TACGCCGT   640               Male-sterile   CTGTACTAATCAAATTTTGCCCTAAACGTTTTTGGCTTTGGAGCAG   641       AG   CAATCACGGCGTAC   T   AATCGGAGCTAGGAGGAGATTCCTCTCCCT             Arabidopsis thaliana     TGAGGAAATCTGGGAGAGGAAAGATCGAAA           Gln36Term   TTTCGATCTTTCCTCTCCCAGATTTCCTCAAGGGAGAGGAATCTCC   642       CAA-TAA   TCCTAGCTCCGATT   A   GTACGCCGTGATTGCTGCTCCAAAGCCAAA               AACGTTTAGGGCAAAATTTGATTAGTACAG               CGGCGTAC   T   AATCGGAG   643           CTCCGATT   A   GTACGCCG   644               Male-sterile   ACTAATCAAATTTTGCCCTAAACGTTTTTGGCTTTGGAGCAGCAAT   645       AG   CACGGCGTACCAAT   A   GGAGCTAGGAGGAGATTCCTCTCCCTTGA             Arabidopsis thaliana     GGAAATCTGGGAGAGGAAAGATCGAAATCAA           Ser37Term   TTGATTTCGATCTTTCCTCTCCCAGATTTCCTCAAGGGAGAGGAAT   646       TCG-TAG   CTCCTCCTAGCTCC   T   ATTGGTACGCCGTGATTGCTGCTCCAAAGC               CAAAAACGTTTAGGGCAAAATTTGATTAGT               GTACCAAT   A   GGAGCTAG   647           CTAGCTCC   T   ATTGGTAC   648               Male-sterile   TAATCAAATTTTGCCCTAAACGTTTTTGGCTTTGGAGCAGCAATCA   649       AG   CGGCGTACCAATCG   T   AGCTAGGAGGAGATTCCTCTCCCTTGAGGA         Arabidopsis thalana     AATCTGGGAGAGGAAAGATCGAAATCAAAC           Glu38Term   GTTTGATTTCGATCTTTCCTCTCCCAGATTTCCTCAAGGGAGAGGA   650       GAG-TAG   ATCTCCTCCTAGCT   A   CGATTGGTACGCCGTGATTGCTGCTCCAAA               GCCAAAAACGTTTAGGGCAAAATTTGATTA               ACCAATCG   T   AGCTAGGA   651           TCCTAGCT   A   CGATTGGT   652               Male-sterile   CTCTCCCACTTCTTTTCGGTGGTTTATTCATTTGGTGACGATATCA   653       AG   CAGAAGCAATGGAT   T   AAGGTGGGAGTAGTCACGATGCAGAGAGT             Brassica napus     AGCAAGAAGATAGGTAGAGGGAAGATAGAGA           Glu3Term   TCTCTATCTTCCCTCTACCTATCTTCTTGCTACTCTCTGCATCGTGA   654       GAA-TAA   CTACTCCCACCTT   A   ATCCATTGCTTCTGTGATATCGTCACCAAATG               AATAAACCACCGAAAAGAAGTGGGAGAG               CAATGGAT   T   AAGGTGGG   655           CCCACCTT   A   ATCCATTG   656               Male-sterile   TATTCATTTGGTGACGATATCACAGAAGCAATGGATGAAGGTGGG   657       AG   AGTAGTCACGATGCA   T   AGAGTAGCAAGAAGATAGGTAGAGGGAA             Brassica napus     GATAGAGATAAAGAGGATAGAGAACACAACAA           Glu11Term   TTGTTGTGTTCTCTATCCTCTTTATCTCTATCTTCCCTCTACCTATC   658       GAG-TAG   TTCTTGCTACTCT   A   TGCATCGTGACTACTCCCACCTTCATCCATTG               CTTCTGTGATATCGTCACCAAATGAATA               ACGATGCA   T   AGAGTAGC   659           GCTACTCT   A   TGCATCGT   660               Male-sterile   GGTGACGATATCACAGAAGCAATGGATGAAGGTGGGAGTAGTCA   661       AG   CGATGCAGAGAGTAGC   T   AGAAGATAGGTAGAGGGAAGATAGAGA             Brassica napus     TAAAGAGGATAGAGAACACAACAAATCGTCAAG           Lys14Term   CTTGACGATTTGTTGTGTTCTCTATCCTCTTTATCTCTATCTTCCCT   662       AAG-TAG   GTACCTATCTTCT   A   GCTACTCTCTGCATCGTGACTACTCCCACCTT               CATCCATTGCTTCTGTGATATCGTCACC               AGAGTAGC   T   AGAAGATA   663           TATCTTCT   A   GCTAGTCT   664               Male-sterile   GACGATATCACAGAAGCAATGGATGAAGGTGGGAGTAGTCACGA   665       AG   TGCAGAGAGTAGCAAG   T   AGATAGGTAGAGGGAAGATAGAGATAAA             Brassica napus     GAGGATAGAGAACACAACAAATCGTCAAGTAA           Lys15Term   TTACTTGACGATTTGTTGTGTTCTCTATCCTCTTTATCTCTATCTTC   666       AAG-TAG   CCTCTACCTATCT   A   CTTGCTACTCTCTGCATCGTGACTACTCCCAC               CTTCATCCATTGCTTCTGTGATATCGTC               GTAGCAAG   T   AGATAGGT   667           ACCTATCT   A   CTTGCTAC   668               Male-sterile   CAACCAAAAAACTTAAAAATCTTCTCTTTCCTTTCCTTACAAGGTGA   669       AG   AGTAATGGACTTC   T   AAAGTGATCTAACCAGAGAGATCTCACCACAA             Lycopersicon     AGGAAACTAGGAAGGGGGAAAATTGAGA             esculentum     TCTCAATTTTCCCCCTTCCTAGTTTCCTTTGTGGTGAGATCTCTCT   670       Glu4Term   GGTTAGATCACTTT   A   GAAGTCCATTACTTCACCTTGTAAGGAAAGG           CAA-TAA   AAAGAGAAGATTTTTAAGTTTTTTGGTTG               TGGACTTC+E,unc  T AAAGTGAT   671           ATCACTTT   A   GAAGTCCA   672               Male-sterile   AAAATCTTCTCTTTCCTTTCCTTACAAGGTGAAGTAATGGACTTCC   673       AG   AAAGTGATCTAACC   T   GAGAGATCTCACCACAAAGGAAACTAGGAA             Lycopersicon     GGGGGAAAATTGAGATCAAAAGGATCGAAA             esculentum     TTTCGATCCTTTTGATCTCAATTTTCCCCCTTCCTAGTTTCCTTTGT   674       Arg9Term   GGTGAGATCTCTC   A   GGTTAGATCACTTTGGAAGTCCATTACTTCAC           AGA-TGA   CTTGTAAGGAAAGGAAAGAGAAGATTTT               ATCTAACC   T   GAGAGATC   675           GATCTCTC   A   GGTTAGAT   676               Male-sterile   ATCTTCTCTTTCCTTTCCTTACAAGGTGAAGTAATGGACTTCCAAA   677       AG   GTGATCTAACCAGA   T   AGATCTCACCACAAAGGAAACTAGGAAGGG             Lycopersicon     GGAAAATTGAGATCAAAAGGATCGAAAACA             esculentum     TGTTTTCGATCCTTTTGATCTCAATTTTCCCCCTTCCTAGTTTCCTT   678       Glu10Term   TGTGGTGAGATCT   A   TCTGGTTAGATCACTTTGGAAGTCCATTACTT           GAG-TAG   CACCTTGTAAGGAAAGGAAAGAGAAGAT               TAACCAGA   T   AGATCTCA   679           TGAGATCT   A   TCTGGTTA   680               Male-sterile   CTTTCCTTTCCTTACAAGGTGAAGTAATGGACTTCCAAAGTGATCT   681       AG   AACCAGAGAGATCT   G   ACCACAAAGGAAACTAGGAAGGGGGAAAA             Lycopersicon     TTGAGATCAAAAGGATCGAAAACACGACGAA             esculentum     TTCGTCGTGTTTTCGATCCTTTTGATCTCAATTTTCCCCCTTCCTAG   682       Ser12Term   TTTCCTTTGTGGT   C   AGATCTCTGTGGTTAGATCACTTTGGAAGTCC       TCA-TGA   ATTACTTCACCTTGTAAGGAAAGGAAAG               AGAGATCT   G   ACCACAAA   683           TTTGTGGT   C   AGATCTCT   684               Male-sterile   GTACTCTCTATTTTCATCTTCCAACCCTTTCTTTCCTTACCAGGTGA   685       NAG1   AAGTATGGACTTC   T   AAAGTGATCTAACAAGAGAGATCTCTCCACAA             Nicotiana tabacum     AGGAAACTGGGAAGAGGAAAGATTGAGA           Gln4Term   TCTCAATCTTTCCTCTTCCCAGTTTCCTTTGTGGAGAGATCTCTCTT   686       CAA-TAA   GTTAGATCACTTT   A   GAAGTCCATACTTTCACCTGGTAAGGAAAGAA               AGGGTTGGAAGATGAAAATAGAGAGTAC               TGGACTTC   T   AAAGTGAT   687           ATCACTTT   A   GAAGTCCA   688               Male-sterile   ATCTTCCAACCCTTTCTTTCCTTACCAGGTGAAAGTATGGACTTCC   689       NAG1   AAAGTGATCTAACA   T   GAGAGATCTCTCCACAAAGGAAACTGGGAA             Nicotiana tabacum     GAGGAAAGATTGAGATCAAACGGATCGAAA           Arg9Term   TTTCGATCCGTTTGATCTCAATCTTTCCTCTTCCCAGTTTCCTTTGT   690       AGA-TGA   GGAGAGATCTCTC   A   TGTTAGATCACTTTGGAAGTCCATACTTTCAC               CTGGTAAGGAAAGAAAGGGTTGGAAGAT               ATCTAACA   T   GAGAGATC   691           GATCTCTC   A   TGTTAGAT   692               Male-sterile   TTCCAACCCTTTCTTTCCTTAGCAGGTGAAAGTATGGACTTCCAAA   693       NAG1   GTGATCTAACAAGA   T   AGATCTCTCCACAAAGGAAACTGGGAAGAG             Nicotiana tabacum     GAAAGATTGAGATCAAACGGATCGAAAACA           Glu10Term   TGTTTTCGATCCGTTTGATCTCAATCTTTCCTCTTCCCAGTTTCCTT   694       GAG-TAG   TGTGGAGAGATCT   A   TCTTGTTAGATGACTTTGGAAGTCCATACTTT               CACCTGGTAAGGAAAGAAAGGGTTGGAA               TAACAAGA   T   AGATCTCT   695           AGAGATCT   A   TCTTGTTA   696               Male-sterile   CTTTCCTTACCAGGTGAAAGTATGGACTTCCAAAGTGATCTAACAA   697       NAG1   GAGAGATCTCTCCA   T   AAAGGAAACTGGGAAGAGGAAAGATTGAGA             Nicotiana tabacum     TCAAACGGATCGAAAACACAACGAATCGTC           Gln14Term   GACGATTCGTTGTGTTTTCGATCCGTTTGATCTCAATCTTTCCTCTT   698       CAA-TAA   CCCAGTTTCCTTT   A   TGGAGAGATCTCTCTTGTTAGATCACTTTGGA               AGTCCATACTTTCACCTGGTAAGGAAAG               TCTCTCCA   T   AAAGGAAA   699           TTTCCTTT   A   TGGAGAGA   700               Male-sterile   GCCTATGAAAACAAACCCAACACGGTCCTGGACGCTGATGCCCAA   701       AG   AGAAGATTGGGAAGG   T   GAAAGATCGAGATCAAGCGGATCGAAAA             Rosa hybrida     CACCACCAATCGTCAAGTCACCTTCTGCAAAA           Gly22Term   TTTTGCAGAAGGTGACTTGACGATTGGTGGTGTTTTCGATCCGCT   702       GGA-TGA   TGATCTGGATCTTTC   A   CCTTCCCAATCTTCTTTGGGCATCAGCGTC               CAGGACCGTGTTGGGTTTGTTTTCATAGGC               TGGGAAGG   T   GAAAGATC   703           GATCTTTC   A   CCTTCCCA   704               Male-sterile   TATGAAAACAAACCCAACACGGTCCTGGACGCTGATGCCCAAAGA   705       AG   AGATTGGGAAGGGGA   T   AGATCGAGATCAAGCGGATCGAAAACAC             Rosa hybrida     CACCAATCGTCAAGTCACCTTCTGCAAAAGGC           Lys23Term   GCCTTTTGCAGAAGGTGACTTGACGATTGGTGGTGTTTTCGATCC   706       AAG-TAG   GCTTGATCTCGATCT   A   TCCCCTTCCCAATCTTCTTTGGGCATCAGC               GTCCAGGACCGTGTTGGGTTTGTTTTCATA               GAAGGGGA   T   AGATCGAG   707           CTCGATCT   A   TCCCCTTC   708               Male-sterile   AACAAACCCAACACGGTCCTGGACGCTGATGCCCAAAGAAGATTG   709       AG   GGAAGGGGAAAGATC   T   AGATCAAGCGGATCGAAAACACCACCAA             Rosa hybrida     TCGTCAAGTCACCTTCTGCAAAAGGCGCAATG           Glu25Term   CATTGCGCCTTTTGCAGAAGGTGACTTGACGATTGGTGGTGTTTT   710       GAG-TAG   CGATCCGCTTGATCT   A   GATCTTTCCCCTTCCCAATCTTCTTTGGGC               ATCAGCGTCCAGGACCGTGTTGGGTTTGTT               GAAAGATC   T   AGATCAAG   711           CTTGATCT   A   GATCTTTC   712               Male-sterile   CCCAACACGGTCCTGGACGCTGATGCCCAAAGAAGATTGGGAAG   713       AG   GGGAAAGATCGAGATC   T   AGCGGATCGAAAACACCACCAATCGTCA             Rosa hybrida     AGTCACCTTCTGCAAAAGGCGCAATGGTTTGC           Lys27   GCAAACCATTGCGCCTTTTGCAGAAGGTGACTTGACGATTGGTGG   714       AAG-TAG   TGTTTTCGATCCGCT   A   GATCTCGATCTTTCCCCTTCCCAATCTTCT               TTGGGCATCAGCGTCCAGGACCGTGTTGGG               TCGAGATC   T   AGCGGATC   715           GATCCGCT   A   GATCTCGA   716               Male-sterile   CAATTGCGTGTTTTTATTTTTTTTGTTTTTGACTAAGTAGAAATGGC   717       far   GTCTCTAAGCGAT   T   AATCGACCGAGGTATCGCGCGAGAGGAAAAT             Antirrhinum majus     CGGGAGAGGAAAGATCGAGATCAAACGGA           Gln7Term   TCCGTTTGATCTCGATCTTTCCTCTCCCGATTTTCCTCTCGGGCGA   718       CAA-TAA   TACCTCGGTCGATT   A   ATCGCTTAGAGACGCCATTTCTACTTAGTCA               AAAAGAAAAAAAATAAAAACAGGCAATTG               TAAGCGAT   T   AATCGACC   719           GGTCGATT   A   ATCGCTTA   720               Male-sterile   GTTTTTATTTTTTTTCTTTTTGACTAAGTAGAAATGGCGTCTCTAAG   721       far   CGATCAATCGACC   T   AGGTATCGCCCGAGAGGAAAATCGGGAGAG             Antirrhinum majus     GAAAGATCGAGATCAAACGGATCGAAAACA           Glu10Term   TGTTTTCGATCCGTTTGATCTCGATCTTTCCTCTCCCGATTTTCCTC   722       GAG-TAG   TCGGGCGATACCT   A   GGTCGATTGATCGCTTAGAGACGCCATTTCT               ACTTAGTCAAAAAGAAAAAAAATAAAAAC               AATCGACC   T   AGGTATCG   723           CGATACCT   A   GGTCGATT   724               Male-sterile   TTTCTTTTTGACTAAGTAGAAATGGCGTCTCTAAGCGATCAATCGA   725       far   CCGAGGTATCGCCC   T   AGAGGAAAATCGGGAGAGGAAAGATCGAG             Antirrhinum majus     ATCAAACGGATCGAAAACAAAACAAATCAAC           Glu14Term   GTTGATTTGTTTTGTTTTCGATCCGTTTGATCTCGATCTTTCCTCTC   726       GAG-TAG   CCGATTTTCCTCT   A   GGGCGATACCTCGGTCGATTGATCGCTTAGA               GACGCCATTTCTACTTAGTCAAAAAGAAA               TATCGCCC   T   AGAGGAAA   727           TTTCCTCT   A   GGGCGATA   728               Male-sterile   TTTGACTAAGTAGAAATGGCGTCTCTAAGCGATCAATCGACCGAG   729       far   GTATCGCCCGAGAGG   T   AAATCGGGAGAGGAAAGATCGAGATCAA             Antirrhinum majus     ACGGATCGAAAACAAAACAAATCAACAGGTTA           Lys16Term   TAACCTGTTGATTTGTTTTGTTTTCGATCCGTTTGATCTCGATCTTT   730       AAA-TAA   CCTCTCCCGATTT   A   CCTCTCGGGCGATACCTCGGTCGATTGATCG               CTTAGAGACGCCATTTCTACTTAGTCAAA               CCGAGAGG   T   AAATCGGG   731           CCCGATTT   A   CCTCTCGG   732               Male-sterile   TGTCCAAGCATTATCAGTCACCACTCACAAGAATGATTAAGGAAGA   733       AG   AGGAAAGGGTAAGT   A   GCAAATAAAGGGGATGTTCCAGAATCAAGA             Cucumis sativus     AGAGAAGATGTCAGACTCGCCTCAGAGGAA           Leu21Term   TTCCTCTGAGGCGAGTCTGACATCTTCTCTTCTTGATTCTGGAACA   734       TTG-TAG   TCCCCTTTATTTGC   T   ACTTACCCTTTCCTTCTTCCTTAATCATTCTT               GTGAGTGGTGACTGATAATGCTTGGACA               GGGTAAGT   A   GCAAATAA   735           TTATTTGC   T   ACTTACCC   736               Male-sterile   TCCAAGCATTATCAGTCACCACTCACAAGAATGATTAAGGAAGAA   737       AG   GGAAAGGGTAAGTTG   T   AAATAAAGGGGATGTTCCAGAATCAAGAA             Cucumis sativus     GAGAAGATGTCAGACTCGCCTCAGAGGAAGA           Gln22Term   TCTTCCTCTGAGGCGAGTCTGACATCTTCTCTTCTTGATTCTGGAA   738       CAA-TAA   CATCCCCTTTATTT   A   CAACTTACCCTTTCCTTCTTCCTTAATCATTC               TTGTGAGTGGTGACTGATAATGCTTGGA               GTAAGTTG   T   AAATAAAG   739           CTTTATTT   A   CAACTTAC   740               Male-sterile   CATTATCAGTCACCACTCACAAGAATGATTAAGGAAGAAGGAAAG   741       AG   GGTAAGTTGCAAAT   A   TAGGGGATGTTCCAGAATCAAGAAGAGAAG             Cucumis sativus     ATGTCAGACTCGCCTCAGAGGAAGATGGGAA           Lys24Term   TTCCCATCTTCCTCTGAGGCGAGTCTGACATCTTCTCTTCTTGATT   742       AAG-TAG   CTGGAACATCCCCT   A   TATTTGCAACTTACCCTTTCCTTCTTCCTTAA               TCATTCTTGTGAGTGGTGACTGATAATG               TGCAAATA   T   AGGGGATG   743           CATCCCCT   A   TATTTGCA   744               Male-sterile   CCACTCACAAGAATGATTAAGGAAGAAGGAAAGGGTAAGTTGCAA   745       AG   ATAAAGGGGATGTTC   T   AGAATCAAGAAGAGAAGATGTCAGACTCG             Cucumis sativus     CCTCAGAGGAAGATGGGAAGAGGAAAGATTG           Gln28Term   CAATCTTTCCTCTTCCCATCTTCCTCTGAGGCGAGTCTGACATCTT   746       CAG-TAG   CTCTTCTTGATTCT   A   GAACATCCCCTTTATTTGCAACTTACCCTTTC               CTTCTTCCTTAATCATTCTTGTGAGTGG               GGATGTTC   T   AGAATCAA   747           TTGATTCT   A   GAACATCC   748               Male-sterile   CCACCACCACCACCACCACCACCACCACACCATGCTCAACATGAT   749       AG   GACTGATCTGAGCTG   A   GGGCCGTCGTCCAAGGTCAAGGAGCAGG             Zea mays     TGGCGGCGGCGCCGACGGGCTCCGGCGACAGG           Cys10Term   CCTGTCGCCGGAGCCCGTCGGCGCCGCCGCCACCTGCTCCTTGA   750       TGC-TGA   CCTTGGACGACGGCCC   T   CAGCTCAGATCAGTCATCATGTTGAGCA               TGGTGTGGTGGTGGTGGTGGTGGTGGTGGTGG               CTGAGCTG   A   GGGCCGTC   751           GACGGCCC   T   CAGCTCAG   752               Male-sterile   ACCACCACCACCACCACCACACCATGCTCAACATGATGACTGATC   753       AG   TGAGCTGCGGGCCGT   A   GTCCAAGGTCAAGGAGCAGGTGGCGGC             Zea mays     GGCGCCGACGGGCTCCGGCGACAGGCAGGGGCA           Ser13Term   TGCCCCTGCCTGTCGCCGGAGCCCGTCGGCGCCGCCGCCACCT   754       TCG-TAG   GCTCCTTGACCTTGGAC   T   ACGGCCCGCAGCTCAGATCAGTCATCA               TGTTGAGCATGGTGTGGTGGTGGTGGTGGTGGT               CGGGCCGT   A   GTCCAAGG   755           CCTTGGAC   T   ACGGCCCG   756               Male-sterile   CACCACCACCACCACACCATGCTCAACATGATGACTGATCTGAGC   757       AG   TGCGGGCCGTCGTCC   T   AGGTCAAGGAGCAGGTGGCGGCGGCGC             Zea mays     CGACGGGCTCCGGCGACAGGCAGGGGCAGGGGA           Lys15Term   TCCCCTGCCCCTGCCTGTCGCCGGAGCCCGTCGGCGCCGCCGC   758       AAG-TAG   CACCTGCTCCTTGACCT   A   GGACGACGGCCCGCAGCTCAGATCAG               TCATCATGTTGAGCATGGTGTGGTGGTGGTGGTG               CGTCGTCC   T   AGGTCAAG   759           CTTGACCT   A   GGACGACG   760               Male-sterile   CACCACCACACCATGCTCAACATGATGACTGATCTGAGCTGCGGG   761       AG   CCGTCGTCCAAGGTC   T   AGGAGCAGGTGGCGGCGGCGCCGACGG             Zea mays     GCTCCGGCGACAGGCAGGGGCAGGGGAGAGGCA           Lys17Term   TGCCTCTCCCCTGCCCCTGCCTGTCGCCGGAGCCCGTCGGCGCC   762       AAG-TAG   GCCGCCACCTGCTCCT   A   GACCTTGGACGACGGCCCGCAGCTCAG               ATCAGTCATCATGTTGAGCATGGTGTGGTGGTG               CCAAGGTC   T   AGGAGCAG   763           CTGCTCCT   A   GACCTTGG   764               Male-sterile   TCCTACCTTTTCTCCTTCAGACCTCAAAATCTGTGTGATAGGAACA   765       AG   AGAGCATGCACATC   T   GAGAAGAGGAGGCTACACCATCCACAGTAA             Zea mays     CAGGCATCATGTCGACCCTGACTTCGGCGG           Arg4Term   CCGCCGAAGTCAGGGTCGACATGATGCCTGTTACTGTGGATGGT   766       CGA-TGA   GTAGCCTCCTCTTCTC   A   GATGTGCATGCTCTTGTTCCTATCACACA               GATTTTGAGGTCTGAAGGAGAAAAGGTAGGA               TGCACATC   T   GAGAAGAG   767           CTCTTCTC   A   GATGTGCA   768               Male-sterile   TACCTTTTCTCCTTCAGACCTCAAAATCTGTGTGATAGGAACAAGA   769       AG   GCATGCACATCCGA   T   AAGAGGAGGCTACACCATCCACAGTAACAG             Zea mays     GCATCATGTCGACCCTGACTTCGGCGGGGC           Glu5Term   GCCCCGCCGAAGTCAGGGTCGACATGATGCCTGTTACTGTGGAT   770       GAA-TAA   GGTGTAGCCTCCTCTT   A   TCGGATGTGCATGCTCTTGTTCCTATCAC               ACAGATTTTGAGGTCTGAAGGAGAAAAGGTA               ACATCCGA   T   AAGAGGAG   771           CTCCTCTT   A   TCGGATGT   772               Male-sterile   CTTTTCTCCTTCAGACCTCAAAATCTGTGTGATAGGAACAAGAGCA   773       AG   TGCACATCCGAGAA   T   AGGAGGCTACACCATCCACAGTAACAGGCA             Zea mays     TCATGTCGACCCTGACTTCGGCGGGGCAGC           Glu6Term   GCTGCCCCGCCGAAGTGAGGGTCGACATGATGCCTGTTACTGTG   774       GAG-TAG   GATGGTGTAGCCTCCT   A   TTCTCGGATGTGCATGCTCTTGTTCCTAT               CACACAGATTTTGAGGTCTGAAGGAGAAAAG               TCCGAGAA   T   AGGAGGCT   775           AGCCTCCT   A   TTCTCGGA   776               Male-sterile   TTCTCCTTCAGACCTCAAAATCTGTGTGATAGGAACAAGAGCATG   777       AG   CACATCCGAGAAGAG   T   AGGCTACACCATCCACAGTAACAGGCATC             Zea mays     ATGTCGACCCTGACTTCGGCGGGGCAGCAGA           Glu7Term   TCTGCTGCCCCGCCGAAGTCAGGGTCGACATGATGCCTGTTACT   778       GAG-TAG   GTGGATGGTGTAGCCT   A   CTCTTCTCGGATGTGCATGCTCTTGTTC               CTATCACACAGATTTTGAGGTCTGAAGGAGAA               GAGAAGAG   T   AGGCTACA   779           TGTAGCCT   A   CTCTTCTC   780               Male-sterile   GCTGGGTCAGGATCGTCGGCGGCGGTGGCGGCGGGGAGCAGC   781       AG   GAGAAGATGGGGAGGGGG   T   AGATCGAGATAAAGCGGATCGAGAA             Oryza sativa     CACGACGAACCGGCAGGTGACCTTCTGCAAGCGCC           Lys5Term   GGCGCTTGCAGAAGGTCACCTGCCGGTTCGTCGTGTTCTCGATC   782       AAG-TAG   CGCTTTATCTCGATCT   A   CCCCCTCCCCATCTTCTCGCTGCTCCCC               GCCGCCACCGCCGCCGACGATCCTGACCCAGC               GGAGGGGG   T   AGATCGAG   783           CTCGATCT   A   CCCCCTCC   784               Male-sterile   TCAGGATCGTCGGCGGGGGTGGCGGCGGGGAGCAGCGAGAAGA   785       AG   TGGGGAGGGGGAAGATC   T   AGATAAAGCGGATCGAGAACACGACG             Oryza sativa     AACCGGCAGGTGACCTTCTGCAAGCGCCGCAATG           GTu7Term   CATTGCGGCGCTTGCAGAAGGTCACCTGCCGGTTCGTCGTGTTCT   786       GAG-TAG   CGATCCGCTTTATCT   A   GATCTTCCCCCTCCCCATCTTCTCGCTGCT               CCCCGCCGCCACCGCCGCCGACGATCCTGA               GGAAGATC   T   AGATAAAG   787           CTTTATCT   A   GATCTTCC   788               Male-sterile   TCGTCGGCGGCGGTGGCGGCGGGGAGCAGCGAGAAGATGGGG   789       AG   AGGGGGAAGATCGAGATA   T   AGCGGATCGAGAACACGACGAACCG             Oryza sativa     GCAGGTGACCTTCTGCAAGCGCCGCAATGGCCTCC           Lys9Term   GGAGGCCATTGCGGCGCTTGCAGAAGGTCACCTGCCGGTTCGTC   790       AAG-TAG   GTGTTCTCGATCCGCT   A   TATCTCGATCTTCCCCCTCCCCATCTTCT               CGCTGCTCCCCGCCGCCACCGCCGCCGACGA               TCGAGATA   T   AGCGGATC   791           GATCCGCT   A   TATCTCGA   792               Male-sterile   GCGGTGGCGGCGGGGAGCAGCGAGAAGATGGGGAGGGGGAAG   793       AG   ATCGAGATAAAGCGGATC   T   AGAACACGACGAACCGGCAGGTGAC             Oryza sativa     CTTCTGCAAGCGCCGCAATGGCCTCCTGAAGAAGG           Glu12Term   CCTTCTTCAGGAGGCCATTGCGGCGCTTGCAGAAGGTCACCTGC   794       GAG-TAG   CGGTTCGTCGTGTTCT   A   GATCCGCTTTATCTCGATCTTCCCCCTCC               CCATCTTCTCGCTGCTCCCCGCCGCCACCGC               AGCGGATC   T   AGAACACG   795           CGTGTTCT   A   GATCCGCT   796                    
     [0127]                   TABLE 16                          Oligonucleotides to produce male-sterile plants                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               Male-sterile   GGGAAGAGGGAAAATAGAAATAAAAAGAATAGAGAACTCAAGCAA   797           P1   TAGACAAGTTACATA   G   TCAAAGAGAAGAAATGGTATCATCAAAAAA             Cucumis sativus     GCCAAAGAAATTACTGTTCTTTGCGATGCT           Tyr21Term   AGCATCGCAAAGAACAGTAATTTCTTTGGCTTTTTTGATGATACCAT   798       TAT-TAG   TTCTTCTCTTTGA   C   TATGTAACTTGTCTATTGCTTGAGTTCTCTATTC               TTTTTATTTCTATTTTCCCTCTTCCC               GTTACATA   G   TCAAAGAG   799           CTCTTTGA   C   TATGTAAC   800               Male-sterile   GAAGAGGGAAAATAGAAATAAAAAGAATAGAGAACTCAAGCAATA   801       P1   GACAAGTTACATATT   G   AAAGAGAAGAAATGGTATCATCAAAAAAGC             Cucumis sativus     CAAAGAAATTACTGTTCTTTGCGATGCTCA           Ser22Term   TGAGCATCGCAAAGAACAGTAATTTCTTTGGCTTTTTTGATGATAC   802       TCA-TGA   CATTTCTTCTCTTT   C   AATATGTAACTTGTCTATTGCTTGAGTTCTCTA               TTGTTTTTATTTCTATTTTCCCTCTTC               TACATATT   G   AAAGAGAA   803           TTCTCTTT+E,un  C AATATGTA   804               Male-sterile   AGAGGGAAAATAGAAATAAAAAGAATAGAGAACTCAAGCAATAGAC   805       P1   AAGTTAGATATTCA   T   AGAGAAGAAATGGTATCATCAAAAAAGCCAA             Cucumis sativus     AGAAATTACTGTTCTTTGCGATGCTCAAG           Lys23Term   CTTGAGCATCGCAAAGAACAGTAATTTCTTTGGCTTTTTTGATGATA   806       AAG-TAG   CCATTTCTTCTCT   A   TGAATATGTAACTTGTCTATTGCTTGAGTTCTC               TATTCTTTTTATTTCTATTTTCCCTCT               CATATTCA   T   AGAGAAGA   807           TCTTCTCT   A   TGAATATG   808               Male-sterile   GGGAAAATAGAAATAAAAAGAATAGAGAACTCAAGCAATAGACAAG   809       P1   TTACATATTCAAAG   T   GAAGAAATGGTATCATCAAAAAAGCCAAAGA             Cucumis sativus     AATTACTGTTCTTTGCGATGCTCAAGTTT           Arg24Term   AAACTTGAGCATCGCAAAGAACAGTAATTTCTTTGGCTTTTTTGATG   810       AGA-TGA   ATACCATTTCTTC   A   CTTTGAATATGTAACTTGTCTATTGCTTGAGTT               CTCTATTCTTTTTATTTCTATTTTCCC               ATTCAAAG   T   GAAGAAAT   811           ATTTCTTC   A   CTTTGAAT   812               Male-sterile   GGGACGTGGGAAGGTTGAGATCAAGAGGATTGAGAACTGAAGTAA   813       P1   CAGGCAGGTGACCTA   G   TCCAAGAGGAGGAATGGGATTATCAAGAA             Malus domestica     GGCAAAGGAGATCACTGTTCTATGTGATGCT           Tyr21Term   AGCATCACATAGAACAGTGATCTCCTTTGCCTTCTTGATAATCCCA   814       TAG-TAG   TTCCTCCTCTTGGA   C   TAGGTGACCTGCCTGTTACTTGAGTTCTCAA               TCCTCTTGATCTCAACCTTCCCACGTCGC               GTGACCTA   G   TGCAAGAG   815           CTCTTGGA   C   TAGGTCAC   816               Male-sterile   CGTGGGAAGGTTGAGATCAAGAGGATTGAGAACTCAAGTAACAGG   817       P1   CAGGTGACCTACTCC   T   AGAGGAGGAATGGGATTATCAAGAAGGCA             Malus domestica     AAGGAGATCACTGTTCTATGTGATGCTAAAG           Lys23Term   CTTTAGCATCACATAGAACAGTGATCTCCTTTGCCTTCTTGATAATC   818       AAG-TAG   CCATTCCTCCTCT   A   GGAGTAGGTCACCTGCCTGTTACTTGAGTTCT               CAATCCTCTTGATCTCAACCTTCCCACG               CCTACTCC   T   AGAGGAGG   819           CCTCCTCT   A   GGAGTAGG   820               Male-sterile   AGGATTGAGAAGTCAAGTAACAGGCAGGTGACCTACTCCAAGAGG   821       P1   AGGAATGGGATTATC   T   AGAAGGCAAAGGAGATGACTGTTCTATGT             Malus domestica     GATGCTAAAGTATCTCTTATCATTTATTCTA           Lys30Term   TAGAATAAATGATAAGAGATACTTTAGCATCACATAGAACAGTGAT   822       AAG-TAG   CTCCTTTGCCTTCT   A   GATAATCGCATTCCTCCTCTTGGAGTAGGTC               ACCTGCCTGTTACTTGAGTTCTCAATCCT               GGATTATC   T   AGAAGGCA   823           TGCCTTCT   A   GATAATCC   824               Male-sterile   ATTGAGAACTCAAGTAACAGGCAGGTGACCTACTCCAAGAGGAGG   825       P1   AATGGGATTATCAAG   T   AGGCAAAGGAGATCACTGTTCTATGTGATG             Malus domestica     CTAAAGTATCTCTTATCATTTATTCTAGCT           Lys31Term   AGCTAGAATAAATGATAAGAGATACTTTAGCATCACATAGAACAGT   826       AAG-TAG   GATCTCCTTTGCCT   A   CTTGATAATGCCATTCCTCCTCTTGGAGTAG               GTCACCTGCCTGTTACTTGAGTTCTCAAT               TTATCAAG   T   AGGCAAAG   827           CTTTGCCT   A   CTTGATAA   828               Male-sterile   CATTTTTACAATAGTTATCTGCAAACAAAAACAAGAGAGAAAAACAA   829       globosa   AAACAAAAAAATG   T   GAAGAGGAAAAATTGAGATCAAAAGAATTGAG             Antirrhinum majus     AACTCAAGCAACAGGCAGGTTACTTACT           Gly2Term   AGTAAGTAACCTGCCTGTTGCTTGAGTTCTCAATTCTTTTGATCTCA   830       GGA-TGA   ATTTTTCCTCTTC   A   CATTTTTTTGTTTTTGTTTTTCTCTCTTGTTTTTG               TTTGCAGATAACTATTGTAAAAATG               AAAAAATG   T   GAAGAGGA   831           TCCTCTTC   A   CATTTTTT   832               Male-sterile   TTTTACAATAGTTATCTGCAAACAAAAACAAGAGAGAAAAACAAAAA   833       globosa   CAAAAAAATGGGA   T   GAGGAAAAATTGAGATCAAAAGAATTGAGAAC             Antirrhinum majus     TCAAGCAACAGGCAGGTTACTTACTCAA           Arg3Term   TTGAGTAAGTAACCTGCCTGTTGCTTGAGTTCTCAATTCTTTTGATC   834       AGA-TGA   TCAATTTTTCCTC   A   TCCCATTTTTTTGTTTTTGTTTTTCTCTCTTGTTT               TTGTTTGCAGATAACTATTGTAAAA               AAATGGGA   T   GAGGAAAA   835           TTTTCCTC   A   TCCCATTT   836               Male-sterile   TACAATAGTTATCTGCAAACAAAAACAAGAGAGAAAAACAAAAACA   837       globosa   AAAAAATGGGAAGA   T   GAAAAATTGAGATCAAAAGAATTGAGAACTC             Antirthinum majus     AAGCAACAGGCAGGTTACTTACTCAAAGA           Gly4Term   TCTTTGAGTAAGTAACCTGCCTGTTGCTTGAGTTCTCAATTCTTTTG   838       GGA-TGA   ATCTCAATTTTTC   A   TCTTCCCATTTTTTTGTTTTTGTTTTTCTCTCTTG               TTTTTGTTTGCAGATAACTATTGTA               TGGGAAGA   T   GAAAAATT   839           AATTTTTC   A   TCTTCCCA   840               Male-sterile   AATAGTTATCTGCAAACAAAAACAAGAGAGAAAAACAAAAACAAAA   841       globosa   AAATGGGAAGAGGA   T   AAATTGAGATCAAAAGAATTGAGAACTCAAG             Antirrhinum majus     CAACAGGCAGGTTACTTACTCAAAGAGAA           Lys5Term   TTCTCTTTGAGTAAGTAACCTGCCTGTTGCTTGAGTTCTCAATTGTT   842       AAA-TAA   TTGATCTCAATTT   A   TCCTCTTCCCATTTTTTTGTTTTTGTTTTTCTCT               CTTGTTTTTGTTTGCAGATAACTATT               GAAGAGGA   T   AAATTGAG   843           CTCAATTT   A   TCCTCTTC   844               Male-sterile   GCTGAGCTCTTGCTGCCCTTGGATCTGTTTGGGAGTGGAGAACGC   845       P1   AGTATGGGGCGCGGC   T   AGATCAAGATCAAGAGGATCGAGAACTCT             Zea mays     ACCAACCGGCAGGTGACCTTCTCCAAGCGCC           Lys5Term   GGCGCTTGGAGAAGGTCACCTGCCGGTTGGTAGAGTTCTCGATCC   846       AAG-TAG   TCTTGATCTTGATCT   A   GCCGCGCCCCATACTGCGTTCTCCACTCCC               AAACAGATCCAAGGGCAGCAAGAGCTCAGC               GGCGCGGC   T   AGATGAAG   847           CTTGATCT   A   GCCGCGCC   848               Male-sterile   CTCTTGCTGCCCTTGGATCTGTTTGGGAGTGGAGAACGCAGTATG   849       P1   GGGCGCGGCAAGATC   T   AGATCAAGAGGATCGAGAACTCTACCAAC             Zea mays     CGGCAGGTGACCTTCTCCAAGCGCCGGGCCG           Lys7Term   CGGCCCGGCGCTTGGAGAAGGTCACCTGCCGGTTGGTAGAGTTC   850       AAG-TAG   TCGATCCTCTTGATCT   A   GATCTTGCCGCGCCCCATACTGCGTTCTC               CACTCCCAAACAGATCCAAGGGCAGCAAGAG               GCAAGATC   T   AGATCAAG   851           CTTGATCT   A   GATCTTGC   852               Male-sterile   CTCTTGCTGCCCTTGGATCTGTTTGGGAGTGGAGAACGCAGTATG   853       P1   GGGCGCGGCAAGATC   T   AGATCAAGAGGATCGAGAACTCTACCAAC             Zea mays     CGGCAGGTGACCTTCTCCAAGCGCCGGGCCG           Lys9Term   CGGCCCGGCGCTTGGAGAAGGTCACCTGCCGGTTGGTAGAGTTC   854       AAG-TAG   TCGATCCTCTTGATCT   A   GATCTTGCCGCGCCCCATACTGCGTTCTC               CACTCCCAAACAGATCCAAGGGCAGCAAGAG               GCAAGATC   T   AGATCAAG   855           GTTGATCT   A   GATCTTGC   856               Male-sterile   GATCTGTTTGGGAGTGGAGAACGCAGTATGGGGCGCGGCAAGAT   857       P1   CAAGATCAAGAGGATC   T   AGAACTCTACCAACCGGCAGGTGACCTT             Zea mays     CTCCAAGCGCCGGGCCGGACTGGTCAAGAAGG           Glu12Term   CCTTCTTGACGAGTCCGGCCCGGCGCTTGGAGAAGGTCACCTGC   858       GAG-TAG   CGGTTGGTAGAGTTCT   A   GATCCTCTTGATCTTGATCTTGCCGCGCC               CCATACTGCGTTCTCCACTCCCAAACAGATC               AGAGGATC   T   AGAACTCT   859           AGAGTTCT   A   GATGCTCT   860               Male-sterile   GCTGAGCTCTTGCTGCCCTTGAATCTGTTAGGGAGTGGAGAACGG   861       P1   AGTATGGGGCGCGGC   T   AGATCGAGATCAAGAGGATCGAGAACTCT             Zea mays     ACCAACCGGCAGGTGACCTTCTCCAAGCGCC           Lys5Term   GGCGCTTGGAGAAGGTCACCTGCCGGTTGGTAGAGTTCTCGATCC   862       AAG-TAG   TCTTGATCTCGATCT   A   GCCGCGCCCCATACTCCGTTCTCCACTCCC               TAACAGATTCAAGGGCAGCAAGAGCTCAGC               GGCGCGGC   T   AGATCGAG   863           CTCGATCT   A   GCCGCGCC   864               Male-sterile   CTCTTGCTGCCCTTGAATCTGTTAGGGAGTGGAGAACGGAGTATG   865       P1   GGGCGCGGCAAGATC   T   AGATCAAGAGGATCGAGAACTCTACCAAC             Zea mays     CGGCAGGTGACCTTCTCCAAGCGCCGGGCCG           Glu7Term   CGGCCCGGCGCTTGGAGAAGGTCACCTGCCGGTTGGTAGAGTTC   866       GAG-TAG   TCGATCCTCTTGATCT   A   GATCTTGCCGCGCCCCATACTCCGTTCTC               CACTCCCTAACAGATTCAAGGGCAGCAAGAG               GCAAGATC   T   AGATCAAG   867           CTTGATCT   A   GATCTTGC   868               Male-sterile   CTGCCCTTGAATCTGTTAGGGAGTGGAGAACGGAGTATGGGGCG   869       P1   CGGCAAGATCGAGATC   T   AGAGGATCGAGAACTCTACCAACCGGCA             Zea mays     GGTGACCTTCTCCAAGCGCCGGGCCGGACTGG           Lys9Term   CCAGTCCGGCCCGGCGCTTGGAGAAGGTCACCTGCCGGTTGGTA   870       AAG-TAG   GAGTTCTCGATCCTCT   A   GATCTCGATCTTGCCGCGCCCCATACTC               CGTTCTCCACTCCCTAACAGATTCAAGGGCAG               TCGAGATC   T   AGAGGATC   871           GATCCTCT   A   GATCTCGA   872               Male-sterile   AATCTGTTAGGGAGTGGAGAACGGAGTATGGGGCGCGGCAAGAT   873       P1   GGAGATGAAGAGGATC   T   AGAACTCTACCAACCGGCAGGTGACCTT             Zea mays     CTCCAAGCGCCGGGCCGGACTGGTCAAGAAGG           Glu12Term   CCTTCTTGACCAGTCCGGCCCGGCGCTTGGAGAAGGTCACCTGC   874       GAG-TAG   CGGTTGGTAGAGTTCT   A   GATCCTCTTGATCTCGATCTTGCCGCGC               CCCATACTCCGTTCTCCACTCCCTAACAGATT               AGAGGATC   T   AGAACTCT   875           AGAGTTCT   A   GATCCTCT   876               Male-sterile   TTGCTGCTAAGCTAGCTGGAGGAAGGAGGAGGAGGAGGAGGAGG   877       P1   CGGGATGGGGCGCGGG+E,un  T AGATCGAGATCAAGAGGATCGAGAACT             Oryza sativa     CCACCAACCGCCAGGTGACCTTCTCCAAGCGCA           Lys5Term   TGCGCTTGGAGAAGGTCACCTGGCGGTTGGTGGAGTTCTCGATCC   878       AAG-TAG   TCTTGATGTCGATGT   A   CCCGCGCCCCATCCCGCCTCCTCCTCCTC               CTCCTCCTTCCTCCAGCTAGCTTAGCAGCAA               GGCGCGGG   T   AGATCGAG   879           CTCGATCT   A   CCCGCGCC   880               Male-sterile   CTAAGCTAGCTGGAGGAAGGAGGAGGAGGAGGAGGAGGCGGGA   881       P1   TGGGGCGCGGGAAGATC   T   AGATCAAGAGGATCGAGAACTCCACC             Oryza sativa     AACCGCCAGGTGACCTTCTCCAAGCGCAGGAGCG           Glu7Term   CGCTCCTGCGCTTGGAGAAGGTCACCTGGCGGTTGGTGGAGTTCT   882       GAG-TAG   CGATCCTCTTGATCT   A   GATCTTCCCGCGCCCCATCCCGCCTCCTC               CTCCTCCTCCTCCTTCCTCCAGCTAGCTTAG               GGAAGATC   T   AGATCAAG   883           CTTGATCT   A   GATCTTCC   884               Male-sterile   TAGCTGGAGGAAGGAGGAGGAGGAGGAGGAGGCGGGATGGGGC   885       P1   GCGGGAAGATCGAGATC   T   AGAGGATCGAGAACTCCACCAACCGC             Oryza sativa     CAGGTGACCTTCTCCAAGCGCAGGAGCGGGATCC           Lys9Term   GGATCCCGCTCCTGCGCTTGGAGAAGGTCACCTGGCGGTTGGTG   886       AAG-TAG   GAGTTCTCGATCCTCT   A   GATCTCGATCTTCCCGCGCCCCATCCCG               CCTCCTCCTCCTCCTCCTCCTTCCTCCAGCTA               TCGAGATC   T   AGAGGATC   887           GATCCTCT   A   GATCTCGA   888               Male-sterile   GAAGGAGGAGGAGGAGGAGGAGGCGGGATGGGGCGCGGGAAG   889       P1   ATCGAGATCAAGAGGATC   T   AGAACTCCACCAACCGCCAGGTGACC             Oryza sativa     TTCTCCAAGCGCAGGAGCGGGATCCTCAAGAAGG           Glu12Term   CCTTCTTGAGGATCCCGCTCCTGCGCTTGGAGAAGGTCACCTGGC   890       GAG-TAG   GGTTGGTGGAGTTCT   A   GATCCTCTTGATCTCGATCTTCCCGCGCC               CCATCCCGCCTCCTCCTCCTCCTCCTCCTTC               AGAGGATC   T   AGAACTCC   891           GGAGTTCT   A   GATCCTCT   892                    
     EXAMPLE 7  
     Engineering Plants for Abiotic Stress Tolerance  
     [0128] Environmental stresses, such as drought, increased soil salinity, soil contamination with heavy meals, and extreme temperature, are major factors limiting plant growth and productivity. The worldwide loss in yield of three major cereal crops, rice, maize, and wheat due to water stress (drought) has been estimated to be over ten billion dollars annually and many currently marginal soils could be brought into cultivation if suitable plant varieties were available.  
     [0129] Physiological and biochemical responses to high levels of ionic or nonionic solutes and decreased water potential have been studied in a variety of plants. It is known, for example, that increasing levels of alcohol dehydrogenase can confer enhances flooding resistance in plants. There are also several possible mechanisms to enhance plant salt tolerance. For example, one mechanism underlying the adaptation or tolerance of plants to osmotic stresses is the accumulation of compatible, low molecular weight osmolytes such as sugar alcohols, special amino acids, and glycinebetaine. Such accumulation can be engineered, for example, by removing feedback inhibition on 1-pyrroline-t-carboxylate synthetase, which results in accumulation of proline. Additionally, recent experiments suggest that altering the expression or activity of specific sodium or potassium transporters can confer enhanced salt tolerance.  
     [0130] Plant tolerance of contamination by heavy metals such as lead and aluminum in soils has also been investigated and one mechanism underlying tolerance is the production of dicarboxylic acids such as oxalate and citrate. In addition, individual genes involved in heavy metal sensitivity have been identified.  
     [0131] The attached tables disclose exemplary oligonucleotide base sequences which can be used to generate site-specific mutations that confer stress tolerance in plants.  
                   TABLE 17                          Genome-Altering Oligos Conferring Stress Tolerance                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:                                     Salt Tolerance   CGTCTTTTTGTGTGGTAGTTGGATGTGACGGTTGCTCAAATGCTT   893           P5CS   GTGACCGATAGCAGT   GC   TAGAGATAAGGATTTCAGGAAGCAACTT             Arabidopsis thaliana     AGTGAAACTGTCAAAGCGATGCTGAGGATGA           Phe128Ala   TCATCCTCAGCATCGCTTTGACAGTTTCACTAAGTTGCTTCCTGAA   894       TTT-GCT   ATCCTTATGTCTA   GC   ACTGCTATCGGTCACAAGCATTTGAGCAACC               GTCACATCCAACTACCACACAAAAAGACG               ATAGCAGT   GC   TAGAGAT   895           ATCTCTA   GC   ACTGCTAT   896               Salt Tolerance   GAGAGTATGTTTGACCAGCTGGATGTGACGGCTGCTCAGCTGCTG   897       P5CS 1   GTGAATGACAGTAGT   GC   CAGAGACAAGGAGTTCAGGAAGCAACTT             Brassica napus     AATGAGACAGTGAAGTCCATGCTTGATTTGA           Phe128Ala   TCAAATCAAGCATGGACTTCACTGTCTCATTAAGTTGCTTCCTGAA   898       TTC-GCC   CTCCTTGTCTCTG   GC   ACTACTGTCATTCACCAGCAGCTGAGCAGC               CGTCACATCCAGCTGGTCAAACATAGTGTC               ACAGTAGT   GC   CAGAGAC   899           GTCTCTG   GC   ACTACTGT   900               Salt Tolerance   GAGACTATGTTTGACCAGATGGATGTGACGGTGGCTCAAATGCTG   901       P505 2   GTGACTGATAGCAGT   G   TCAGAGATAAGGATTTCAGGAAGCAACTT             Brassica napus     AGTGAGACAGTCAAAGCTATGCTGAAAATGA           Phe129Ala   TCATTTTCAGCATAGCTTTGACTGTCTCACTAAGTTGCTTCCTGAA   902       TTC-GCC   ATCCTTATCTCTGA   C   ACTGCTATCAGTCACCAGCATTTGAGCCACC               GTCACATCCATCTGGTCAAACATAGTCTC               ATAGCAGT   G   TCAGAGAT   903           ATCTCTGA   C   ACTGCTAT   904               Salt Tolerance   GATATGTTGTTTAACCAACTGGATGTCTCGTCATCTCAACTTCTTG   905       P5GS   TCACCGACAGTGAT   GC   TGAGAACCCAAAGTTCCGGGAGCAACTCA             Oryza sativa     CTGAAACTGTTGAGTCATTATTAGATCTTA           Phe128Ala   TAAGATCTAATAATGACTCAACAGTTTCAGTGAGTTGCTCCCGGAA   906       TTT-GCT   CTTTGGGTTCTCA   GC   ATCACTGTCGGTGACAAGAAGTTGAGATGA               CGAGACATCCAGTTGGTTAAACAACATATC               ACAGTGAT   GC   TGAGAAC   907           GTTCTCA   GC   ATCACTGT   908               Salt Tolerance   GATATTTTGTTTAGTCAGCTGGATGTGACATCTGCTCAGCTTCTTG   909       P5CS   TTACTGACAATGAT   GC   TAGAGACCAAGATTTTAGAAAGCAACTTTC             Medicago sativa     TGAAACTGTGAGATCACTTCTAGCACTAA           Phe128Ala   TTAGTGCTAGAAGTGATCTCACAGTTTCAGAAAGTTGCTTTCTAAA   910       TTT-GCT   ATCTTGGTCTCTA   GC   ATCATTGTCAGTAAGAAGAAGCTGAGCAGAT               GTCACATCCAGCTGACTAAACAAAATATC               ACAATGAT   GC   TAGAGAC   911           GTCTCTA   GC   ATCATTGT   912               Salt Tolerance   GATACATTGTTTAGTCAGCTGGATGTGACATCAGCTCAGCTACTC   913       P5CS   GTTACTGATAATGAT   GC   TAGGGATCCAGAATTCAGGAAGCAACTT             Actinidia deliciosa     ACTGAAACTGTAGAATCACTATTGAATTTGA           Phe128Ala   TCAAATTCAATAGTGATTCTACAGTTTCAGTAAGTTGCTTCCTGAAT   914       TTT-GCT   TCTGGATCCCTA   GC   ATCATTATCAGTAACGAGTAGCTGAGCTGAT               GTCACATCCAGCTGACTAAACAATGTATC               ATAATGAT   GC   TAGGGAT   915           ATCCCTA   GC   ATCATTAT   916               Salt Tolerance   GACACACTCTTCAGTCAACTGGATGTGACATCAGCACAGCTTCTT   917       P5CS   GTAACAGATAATGAC   GC   CAGAAGTCCAGAATTTAGAAAACAACTTA             Cichorium intybus     CTGAAACAGTCGATTCTTTATTATCTTATA           Phe122Ala   TATAAGATAATAAAGAATCGACTGTTTCAGTAAGTTGTTTTCTAAAT   918       TTC-GCC   TCTGGACTTCTG   GC   GTCATTATCTGTTACAAGAAGCTGTGCTGAT               GTCACATCCAGTTGACTGAAGAGTGTGTC               ATAATGAC   GC   CAGAAGT   919           ACTTCTG   GC   GTCATTAT   920               Salt Tolerance   GATTCTTTGTTCAGTCAGTTGGATGTGACATCAGCTCAGCTTCTGG   921       P5CS   TGACTGATAATGAC   GC   TAGAGATCCAGATTTTAGGAGACAACTCA             Lycopersicon     ATGACACAGTAAATTCGTTGCTTTCTCTAA             esculentum     TTAGAGAAAGCAACGAATTTACTGTGTCATTGAGTTGTCTCCTAAA   922       Phe12BAla   ATCTGGATCTCTA   GC   GTCATTATCAGTCACCAGAAGCTGAGCTGA           TTT-GCT   TGTCACATCCAACTGACTGAACAAAGAATC               ATAATGAC   GC   TAGAGAT   923           ATCTCTA   GC   GTCATTAT   924               Salt Tolerance   GATACCATGTTCAGCCAGCTTGATGTGACTTCTTCCCAACTTCTTG   925       P5CS   TGAATGATGGATTT   GC   TAGGGATGCTGGCTTCAGAAAACAACTTT             Vigna unguiculata     CGGACACAGTGAACGCGTTATTAGATTTAA           Phe162Ala   TTAAATCTAATAACGCGTTCACTGTGTCCGAAAGTTGTTTTCTGAA   926       TTT-GCT   GCCAGCATCCCTA   GC   AAATCCATCATTCACAAGAAGTTGGGAAGA               AGTCACATCAAGCTGGCTGAACATGGTATC               ATGGATTT   GC   TAGGGAT   927           ATCCCTA   GC   AAATCCAT   928                Salt Tolerance   GACACCTTGTTTAGTCAGTTGGATCTGACTGCTGCTCAGCTGCTT   929       P5CS   GTGACGGACAACGAC   GC   TAGAGATCCAAGTTTTAGAACACAACTA             Mesembryanthemum     ACTGAAACAGTGTATCAGTTGTTGGATCTAA             crystallinum     TTAGATCCAACAACTGATACACTGTTTCAGTTAGTTGTGTTCTAAA   930       Phe125Ala   ACTTGGATCTCTA   GC   GTCGTTGTCCGTCACAAGCAGCTGAGCAGC           TTT-GCT   AGTCAGATCCAACTGACTAAACAAGGTGTC               ACAACGAC   GC   TAGAGAT   931           ATCTCTA   GC   GTCGTTGT   932               Salt Tolerance   GACACATTATTTAGCCAGCTGGATGTGACATCAGCTCAGCTTCTT   933       P5CS   GTGACTGATAATGAT   GC   TAGGGATGAAGCTTTCCGAAATCAACTTA             Vitis vinifera     CTCAAACAGTGGATTCATTGTTAGCTTTGA           Phe130Ala   TCAAAGCTAACAATGAATCCACTGTTTGAGTAAGTTGATTTCGGAA   934       TTT-GCT   AGCTTCATCCCTA   GC   ATCATTATCAGTCACAAGAAGCTGAGCTGAT               GTCACATCCAGCTGGCTAAATAATGTGTC               ATAATGAT   GC   TAGGGAT   935           ATCCCTA   GC   ATCATTAT   936               Salt Tolerance   GATACGCTGTTCACTCAGCTCGATGTGACATCGGCTCAGCTTCTT   937       P5CS   GTGACGGATAACGAT   GC   TCGAGATAAGGATTTCAGGAAGCAGCTT             Vigna aconitifolia     ACTGAGACTGTGAAGTCGCTGTTGGGGCTGA           Phe129Ala   TCAGCGCCAACAGCGACTTCACAGTCTCAGTAAGCTGCTTCCTGA   938       TTT-GCT   AATCCTTATCTCGA   GC   ATCGTTATCCGTCACAAGAAGCTGAGCCG               ATGTCACATCGAGCTGAGTGAACAGCGTATC               ATAACGAT   GC   TCGAGAT   939           ATCTCGA   GC   ATCGTTAT   940               Salt Tolerance   AGAGATGTTCTTAGTTCCAAAGAAATCTCACCTCTCAGTTTCTCCG   941       HKT1   TCTTCACAACAGTT   GT   CACGTTTGCAAACTGCGGATTTGTCCCCAC             Arabidopsis thaliana     GAATGAGAACATGATCATCTTTCGCAAAA           Ser207Val   TTTTGCGAAAGATGATCATGTTCTCATTCGTGGGGACAAATCCGC   942       TCC-GTC   AGTTTGCAAACGTG   AC   AACTGTTGTGAAGACGGAGAAAGTGAGAG               GTGAGATTTCTTTGGAACTAAGAACATCTCT               CAACAGTT   GT   CACGTTT   943           AAACGTG   AC   AACTGTTG   944               Salt Tolerance   CGAATGAGAACATGATCATCTTTCGCAAAAACTCTGGTCTCATCTG   945       HKT1   GCTCCTAATCCCTC   T   AGTACTGATGGGAAACACTTTGTTCCCTTGC             Arabidopsis thaliana     TTCTTGGTTTTGCTCATATGGGGACTTTA           Gln237Leu   TAAAGTCCCCATATGAGCAAAACCAAGAAGCAAGGGAACAAAGTG   946       CAA-CTA   TTTCCCATCAGTACT   A   GAGGGATTAGGAGCCAGATGAGACCAGAG               TTTTTGCGAAAGATGATCATGTTCTCATTCG               AATCCCTC   T   AGTACTGA   947           TCAGTACT   A   GAGGGATT   948               Salt Tolerance   AGTCTCTAGAAGGAATGAGTTCGTACGAGAAGTTGGTTGGATCGT   949       HKT1   TGTTTCAAGTGGTGA   G   TTCGCGACACACCGGAGAAACTATAGTAG             Arabidopsis thaliana     ACCTCTCTACACTTTCCCCAGCTATCTTGGT           Asn332Ser   ACCAAGATAGCTGGGGAAAGTGTAGAGAGGTCTACTATAGTTTCT   950       AAT-AGT   CCGGTGTGTCGCGAA   C   TCACCACTTGAAACAACGATCCAACCAAC               TTCTCGTACGAACTCATTCCTTCTAGAGACT               AGTGGTGA   G   TTCGCGAC   951           GTCGCGAA   C   TCACCACT   952               Salt Tolerance   AGAGATGTGCTAAAGAAGAAAGGTCTCAAAATGGTGACCTTTTCC   953       HKT1   GTCTTCACCACCGTG   GT   GACCTTTGCCAGTTGTGGGTTTGTCCCG             Eucalyptus     ACCAATGAAAACATGATTATCTTCAGCAAAA             camaldulensis     TTTTGCTGAAGATAATCATGTTTTCATTGGTCGGGACAAACCCACA   954       Ser256Val   ACTGGCAAAGGTC   AC   CACGGTGGTGAAGACGGAAAAGGTCACCA           TCG-GTG   TTTTGAGACCTTTCTTCTTTAGCACATCTCT               CCACCGTG   GT   GACCTTT   955           AAAGGTC   AC   CACGGTGG   956               Salt Tolerance   CCAATGAAAACATGATTATCTTCAGCAAAAACTCTGGCCTCCTCCT   957       HKT1   GATTCTCATCCCTC   T   GGCCCTTCTTGGGAACATGCTGTTCCCATC             Eucalyptus     GAGCCTACGTTTGACGCTTTGGCTCATCGG             camaldulensis     CCGATGAGCCAAAGCGTCAAACGTAGGCTCGATGGGAACAGCAT   958       Gln286Leu   GTTCCCAAGAAGGGCCA   G   AGGGATGAGAATCAGGAGGAGGCCA           CAG-CTG   GAGTTTTTGCTGAAGATAATCATGTTTTCATTGG               CATCCCTC   T   GGCCCTTC   959           GAAGGGCC   A   GAGGGATG   960               Salt Tolerance   AATCGTTGAATGGACTAAGCTCCTGTGAGAAAATCGTGGGCGCGC   961       HKT1   TGTTTCAGTGCGTGA   G   CAGCAGACATACCGGCGAGACGGTCGTC             Eucalyptus     GATCTGTCCACAGTTGCTCCCGCCATCTTGGT             camaldulensis     ACCAAGATGGCGGGAGCAACTGTGGACAGATCGACGACCGTCTC   962       Asn381Ser   GCCGGTATGTCTGCTG   C   TCACGCACTGAAACAGCGCGCCCACGA           AAC-AGC   TTTTCTCACAGGAGCTTAGTCCATTCAACGATT               GTGCGTGA   G   CAGCAGAC   963           GTCTGCTG+E,un  C TCACGCAC   964               Salt Tolerance   AAAGCTCCACTGAAGAAGAAAGGGATCAACATTGCACTCTTCTCA   965       HKT1   TTCTCGGTCACGGTC   GT   CTCGTTTGCGAATGTGGGGCTCGTGCC             Oryza sativa     GACAAATGAGAACATGGCAATCTTCTCCAAGA           Ser238Val   TCTTGGAGAAGATTGCCATGTTCTCATTTGTCGGCACGAGCCCCA   966       TCC-GTC   CATTCGCAAACGAG   AC   GACCGTGACCGAGAATGAGAAGAGTGCA               ATGTTGATCCCTTTCTTCTTCAGTGGAGCTTT               TCACGGTC   GT   CTCGTTT   967           AAACGAG   AC   GACCGTGA   968               Salt Tolerance   CAAATGAGAACATGGCAATCTTCTCCAAGAACCCGGGCCTCCTCC   969       HKT1   TCCTGTTCATCGGCC   T   GATTGTTGCAGGCAATACACTTTACCCTCT             Oryza sativa     CTTCCTAAGGCTATTGATATGGTTCCTGGG           Gln268Leu   CCCAGGAACCATATCAATAGCCTTAGGAAGAGAGGGTAAAGTGTA   970       CAG-CTG   TTGCCTGCAAGAATC   A   GGCCGATGAACAGGAGGAGGAGGCCCGG               GTTCTTGGAGAAGATTGCCATGTTCTCATTTG               CATCGGCC   T   GATTCTTG   971           CAAGAATC   A   GGCCGATG   972               Salt Tolerance   CAGTCTTTGATGGACTCAGCTCTTACCAGAAGATTATCAATGCATT   973       HKT1   GTTCATGGCAGTGA   G   CGCAAGGCACTCGGGGGAGAACTCCATCG             Oryza sativa     ACTGCTCACTCATCGCCCCTGCTGTTCTAGT           Asn363Ser   ACTAGAACAGCAGGGGCGATGAGTGAGCAGTCGATGGAGTTCTC   974       AAC-AGC   CCCCGAGTGCCTTGCG   C   TCACTGCCATGAACAATGCATTGATAAT               CTTCTGGTAAGAGCTGAGTCCATCAAAGACTG               GGCAGTGA   G   CGCAAGGC   975           GCCTTGCG   C   TCACTGCC   976               Salt Tolerance   GTGCCCCACTGAACAAGAAAGGGATCAACATCGTGCTCTTCTCAC   977       HKT1   TATCAGTCACCGTTG   T   CTCCTGTGCGAATGCAGGACTCGTGCCCA             Triticum aestivum     CAAATGAGAACATGGTCATCTTCTCAAAGAA           Ala240Val   TTCTTTGAGAAGATGACCATGTTCTCATTTGTGGGCACGAGTCCT   978       GCC-GTC   GCATTCGCACAGGAG   A   CAACGGTGAGTGATAGTGAGAAGAGCAC               GATGTTGATCCCTTTCTTGTTCAGTGGGGCAC               CACCGTTG   T   CTCCTGTG   979           CACAGGAG   A   CAACGGTG   980               Salt Tolerance   CAAATGAGAACATGGTCATCTTCTCAAAGAATTCAGGCCTCTTGTT   981       HKT1   GCTGCTGAGTGGCC   T   GATGCTCGCAGGCAATACATTGTTCCCTCT             Triticum aestivum     CTTCCTGAGGCTACTGGTGTGGTTCCTGGG           Gln270Leu   CCCAGGAACCACACCAGTAGCCTCAGGAAGAGAGGGAACAATGT   982       CAG-CTG   ATTGCCTGCGAGCATC   A   GGCCACTCAGCAGCAACAAGAGGCCTG               AATTCTTTGAGAAGATGACCATGTTCTCATTTG               GAGTGGCC   T   GATGCTCG   983           CGAGCATC   A   GGCCACTC   984               Salt Tolerance   CAGTCTTTGATGGGCTCAGCTCTTATCAGAAGACTGTCAATGCATT   985       HKT1   CTTCATGGTGGTGA   G   TGCGAGGCACTCAGGGGAGAATTCCATCG             Triticum aestivum     ACTGCTCGCTCATGTCCCCTGCCATTATAGT           Asn365Ser   ACTATAATGGCAGGGGACATGAGCGAGCAGTCGATGGAATTCTCC   986       AAT-AGT   CCTGAGTGCCTCGCA   C   TCACCACCATGAAGAATGCATTGACAGTC               TTCTGATAAGAGCTGAGCCCATCAAAGACTG               GGTGGTGA   G   TGCGAGGC   987           GCCTCGCA   C   TCACCACC   988               Freezing Tolerance   TTTTTTTTGTTTTCGTTTTCAAAAAGAAAATCTTTGAATTTTATGGCA   989       praline oxidase   ACCGGTCTTCTC   T   GAACAAACTTTATCCGGCGATCTTACCGTTTAG           precursor   CCGCTTTTAGCCCGGTGGGTCCTCCCA             Arabidopsis thaliana     TGGGAGGACCCACCGGGCTAAAAGCGGGTAAACGGTAAGATCGC   990       Arg7Term   GGGATAAAGTTTGTTC   A   GAGAAGACGGGTTGCCATAAAATTCAAA           CGA-TGA   GATTTTGTTTTTGAAAACGAAAACAAAAAAAA               GTCTTCTC   T   GAACAAAC   991           GTTTGTTC   A   GAGAAGAC   992               Freezing Tolerance   TCAAAAACAAAATCTTTGAATTTTATGGCAACCCGTCTTCTCAGAA   993       proline oxidase   CAAACTTTATCCGG   T   GATCTTACCGTTTACCGGCTTTTAGCCCGGT           precursor   GGGTCCTCCCACCGTGACTGCTTCCACCG             Arabidopsis thaliana     CGGTGGAAGCAGTCACGGTGGGAGGACCCAGCGGGCTAAAAGC   994       Arg13Term   GGGTAAACGGTAAGATC   A   CCGGATAAAGTTTGTTCTGAGAAGACG           CGA-TGA   GGTTGCCATAAAATTCAAAGATTTTGTTTTTGA               TTATCCGG   T   GATCTTAC   995           GTAAGATC   A   CCGGATAA   996               Freezing Tolerance   AAAATCTTTGAATTTTATGGCAACCCGTCTTCTCCGAACAAACTTT   997       praline oxidase   ATCCGGCGATCTTA   G   CGTTTACCCGCTTTTAGCCCGGTGGGTCCT           precursor   CCCACCGTGACTGCTTCCACCGCCGTCGTC             Arabidopsis thaliana     GACGACGGCGGTGGAAGCAGTCACGGTGGGAGGACCCACCGGG   998       Tyr15Term   CTAAAAGCGGGTAAACG   C   TAAGATCGCCGGATAAAGTTTGTTCGG           TAG-TAG   AGAAGAGGGGTTGCCATAAAATTCAAAGATTTT               CGATCTTA   G   CGTTTACC   999           GGTAAACG   C   TAAGATCG   1000               Freezing Tolerance   CTTTGAATTTTATGGCAACCCGTCTTCTCCGAACAAACTTTATCCG   1001       praline oxidase   GCGATCTTACCGTT   A   ACCCGCTTTTAGCCCGGTGGGTCCTCCCAC           precursor   CGTGACTGCTTCCACCGCCGTCGTCCCGGA             Arabidopsis thaliana     TCCGGGACGACGGCGGTGGAAGCAGTCACGGTGGGAGGACCCA   1002       Leu17Term   CCGGGCTAAAAGCGGGT   T   AACGGTAAGATCGGCGGATAAAGTTT           TTA-TAA   GTTCGGAGAAGACGGGTTGCCATAAAATTCAAAG               TTACCGTT   A   ACCCGCTT   1003           AAGCGGGT   T   AACGGTAA   1004               Freezing Tolerance   CCGGTGGGTCCTCCCACCGTGACTGCTTCCAGCGCCGTGGTCCC   1005       proline oxidase   GGAGATTCTCTCCTTT   T   GACAACAAGCACCGGAACCACCTCTTCA           precursor   CCACCCAAAACCCACCGAGCAATCTCACGATG             Arabidopsis thaliana     CATCGTGAGATTGCTCGGTGGGTTTTGGGTGGTGAAGAGGTGGT   1006       Gly42Term   TCCGGTGCTTGTTGTC   A   AAAGGAGAGAATCTCCGGGACGACGGC           GGA-TGA   GGTGGAAGCAGTCACGGTGGGAGGACCCACCGG               TCTCCTTT   T   GACAACAA   1007           TTGTTGTC   A   AAAGGAGA   1008               Lead Tolerance   ACATGAAGCAGTGAAATCTCTGTTTGTATTGAATCTTATTAGTCTCT   1009       cyclic nucleotide-   AAACTATGAATTTC   T   GACAAGAGAAGTTTGTAAGGTCAGTGTTCCA           regulated ion channel   GATTTGTCTCATTGAATTCTAAGTCGTGA             Arabidopsis thaliana     TCACGACTTAGAATTCAATGAGACAAATCTGGAACACTGACCTTAC   1010       Arg4Term   AAACTTCTCTTGTC   A   GAAATTCATAGTTTGAGACTAATAAGATTCAA           CGA-TGA   TACAAACAGAGATTTCACTGCTTCATGT               TGAATTTC   T   GACAAGAG   1011           CTCTTGTC   A   GAAATTCA   1012               Lead Tolerance   TGAAGCAGTGAAATCTCTGTTTGTATTGAATCTTATTAGTCTCAAA   1013       cyclic nucleotide-   CTATGAATTTCCGA   T   AAGAGAAGTTTGTAAGGTCAGTGTTCCAGAT           regulated ion channel   TTGTCTCATTGAATTCTAAGTCGTGAAGC             Arabidopsis thaliana     GCTTCACGACTTAGAATTCAATGAGACAAATCTGGAACACTGACCT   1014       Gln5Term   TACAAACTTCTCTT   A   TCGGAAATTCATAGTTTGAGACTAATAAGATT           CAA-TAA   CAATACAAACAGAGATTTCACTGCTTCA               ATTTCCGA   T   AAGAGAAG   1015           CTTCTCTT   A   TCGGAAAT   1016               Lead Tolerance   AGCAGTGAAATCTCTGTTTGTATTGAATCTTATTAGTCTCAAACTAT   1017       cyclic nucleotide-   GAATTTCCGACAA   T   AGAAGTTTGTAAGGTCAGTGTTCCAGATTTGT           regulated ion channel   CTCATTGAATTCTAAGTCGTGAAGCTTA             Arabidopsis thaliana     TAAGCTTCACGACTTAGAATTCAATGAGACAAATCTGGAACACTGA   1018       Glu6Term   CCTTACAAACTTCT   A   TTGTCGGAAATTCATAGTTTGAGACTAATAA           GAG-TAG   GATTCAATACAAACAGAGATTTCACTGCT               TCCGACAA   T   AGAAGTTT   1019           AAACTTCT   A   TTGTCGGA   1020               Lead Tolerance   AGTGAAATCTCTGTTTGTATTGAATCTTATTAGTCTCAAACTATGAA   1021       cyclic nucleotide-   TTTCCGACAAGAG   T   AGTTTGTAAGGTCAGTGTTCCAGATTTGTCTC           regulated ion channel   ATTGAATTCTAAGTCGTGAAGCTTAATT             Arabidopsis thaliana     AATTAAGCTTCACGACTTAGAATTCAATGAGACAAATCTGGAACAC   1022       Lys7Term   TGACCTTACAAACT   A   CTCTTGTCGGAAATTCATAGTTTGAGACTAA           AAG-TAG   TAAGATTCAATACAAACAGAGATTTCACT               GACAAGAG   T   AGTTTGTA   1023           TACAAACT   A   CTCTTGTC   1024               Lead Tolerance   CATTGAATTCTAAGTCGTGAAGCTTAATTCGATTCTTCTTCACTTTC   1025       cyclic nucleotide-   TCGGATCAGGTTT   T   AAGATTGGAAGTCGGATAAGACTTCCTCCGA           regulated ion channel   CGTGGAATATTCCGGTAAAAACGAGATTC             Arabidopsis thaliana     GAATCTCGTTTTTACCGGAATATTCCACGTCGGAGGAAGTCTTATC   1026       Gln12Term   CGACTTCCAATCTT   A   AAACCTGATCCGAGAAAGTGAAGAAGAATC           CAA-TAA   GAATTAAGCTTCACGACTTAGAATTCAATG               TCAGGTTT   T   AAGATTGG   1027           CCAATCTT   A   AAACCTGA   1028               Lead Tolerance   TGGAAGTCAATCCCCCACGTTGAGCAGGTTGATGCATTGGGTAAA   1029       cyclic nucleotide-   GTTATGAATCACCGC   T   AAGACGAGTTTGTGAGGTTTCAGGATTGG           gated calmodulin-   AAATCAGAGAGAAGCTCTGAGGGAAATTTTC           binding ion channel   GAAAATTTCCCTCAGAGCTTCTCTCTGATTTCCAATCCTGAAACCT   1030       (CBP4)   CACAAACTCGTCTT   A   GCGGTGATTCATAACTTTAGCCAATGCATCA             Nicotiana Tabacum     ACCTGCTCAACGTGGGGGATTGACTTCCA           Gln5Term   ATCACCGC   T   AAGACGAG   1031       CAA-TAA   CTCGTCTT   A   GCGGTGAT   1032               Lead Tolerance   TCAATCCCCCACGTTGAGCAGGTTGATGCATTGGCTAAAGTTATG   1033       cyclic nucleotide-   AATCACCGCCAAGAC   T   AGTTTGTGAGGTTTCAGGATTGGAAATCA           gated calmodulin-   GAGAGAAGCTCTGAGGGAAATTTTCATGCTA           binding ion channel   TAGCATGAAAATTTCCCTCAGAGCTTCTCTCTGATTTCCAATCCTG   1034       (CBP4)   AAACCTCACAAACT   A   GTCTTGGCGGTGATTCATAACTTTAGCCAAT             Nicotiana Tabacum     GCATCAACCTGCTCAACGTGGGGGATTGA           Gly7Term   GCCAAGAC   T   AGTTTGTG   1035       GAG-TAG   CACAAACT   A   GTCTTGGC   1036               Lead Tolerance   GAGCAGGTTGATGCATTGGCTAAAGTTATGAATCACCGCCAAGAC   1037       cyclic nucleotide-   GAGTTTGTGAGGTTT   T   AGGATTGGAAATCAGAGAGAAGCTCTGAG           gated calmodulin-   GGAAATTTTCATGCTAAAGGTGGAGTCCACC           binding ion channel   GGTGGACTCCACCTTTAGCATGAAAATTTCCCTCAGAGCTTCTCTC   1038       (CBP4)   TGATTTCCAATCCT   A   AAACCTCACAAACTCGTCTTGGCGGTGATTC             Nicotiana Tabacum     ATAACTTTAGCCAATGCATCAACCTGCTC           Gln12Term   TGAGGTTT   T   AGGATTGG   1039       CAG-TAG   CCAATCCT   A   AAACCTCA   1040               Lead Tolerance   TGATGCATTGGCTAAAGTTATGAATCACCGCCAAGACGAGTTTGT   1041       cyclic nucleotide-   GAGGTTTCAGGATTG   T   AAATCAGAGAGAAGCTCTGAGGGAAATTT           gated calmodulin-   TCATGCTAAAGGTGGAGTCCACCGAAGTAAA           binding ion channel   TTTACTTCGGTGGACTCCACCTTTAGCATGAAAATTTCCCTCAGAG   1042       (CBP4)   CTTCTCTCTGATTT   A   CAATCCTGAAACCTCACAAACTCGTCTTGGC             Nicotiana Tabacum     GGTGATTCATAACTTTAGCCAATGCATCA           Trp14Term   CAGGATTG   T   AAATCAGA   1043       TGG-TGA   TCTGATTT   A   CAATCCTG   1044               Lead Tolerance   GATGCATTGGCTAAAGTTATGAATCACCGCCAAGACGAGTTTGTG   1045       cyclic nucleotide-   AGGTTTCAGGATTGG   T   AATCAGAGAGAAGCTGTGAGGGAAATTTT           gated calmoduin-   CATGCTAAAGGTGGAGTCCACCGAAGTAAAG           binding ion channel   CTTTACTTCGGTGGACTCCACCTTTAGCATGAAAATTTCCCTCAGA   1046       (CBP4)   GCTTCTCTCTGATT   A   CCAATCCTGAAACCTCACAAACTCGTCTTGG             Nicotiana Tabacum     CGGTGATTCATAACTTTAGCCAATGCATC           Lys15Term   AGGATTGG   T   AATCAGAG   1047       AAA-TAA   CTCTGATT   A   CCAATCCT   1048               Lead Tolerance   CTTGAAGAATTGATCTACCACTCTTAGCTGCTAACTGTTCGCCTGG   1049       calmoduin binding   TGGAGATAATGATG   T   AAAGAGAGGACAGATATGTTAGATTTCAGG           transport protein   ACTGCAAATCAGAGCAATCTGTTATCTCAG             Hordeum vulgare     CTGAGATAACAGATTGCTCTGATTTGCAGTCCTGAAATGTAACATA   1050       Glu2Term   TCTGTCCTCTCTTT   A   CATCATTATCTCCACCAGGCGAACAGTTAGC           GAA-TAA   AGCTAAGAGTGGTAGATCAATTCTTCAAG               TAATGATG   T   AAAGAGAG   1051           CTCTCTTT   A   CATCATTA   1052               Lead Tolerance   GAAGAATTGATCTACCACTCTTAGCTGCTAACTGTTCGCCTGGTG   1053       calmodulin binding   GAGATAATGATGGAA   T   GAGAGGACAGATATGTTAGATTTCAGGAC           transport protein   TGCAAATCAGAGCAATCTGTTATCTCAGAGA             Hordeum vulgare     TCTCTGAGATAACAGATTGCTCTGATTTGCAGTCCTGAAATCTAAC   1054       Arg3Term   ATATCTGTCCTCTC   A   TTCCATCATTATCTCCACCAGGCGAACAGTT           AGA-TGA   AGCAGCTAAGAGTGGTAGATCAATTCTTC               TGATGGAA   T   GAGAGGAC   1055           GTCCTCTC   A   TTCCATCA   1056               Lead Tolerance   GAATTGATCTACCACTCTTAGCTGCTAACTGTTCGCCTGGTGGAG   1057       calmodulin binding   ATAATGATGGAAAGA   T   AGGACAGATATGTTAGATTTCAGGACTGC           transport protein   AAATCAGAGCAATCTGTTATCTCAGAGAACG             Hordeum vulgare     CGTTCTCTGAGATAACAGATTGCTCTGATTTGCAGTCCTGAAATCT   1058       Glu4Term   AACATATCTGTCCT   A   TCTTTCCATCATTATCTCCACCAGGCGAACA           GAG-TAG   GTTAGCAGCTAAGAGTGGTAGATCAATTC               TGGAAAGA   T   AGGACAGA   1059           TCTGTCCT   A   TCTTTCCA   1060               Lead Tolerance   ATCTACCACTCTTAGCTGCTAACTGTTCGCCTGGTGGAGATAATG   1061       calmodulin binding   ATGGAAAGAGAGGAC   T   GATATGTTAGATTTCAGGACTGCAAATCA           transport protein   GAGCAATCTGTTATCTCAGAGAACGCAGTTT             Hordeum vulgare     AAACTGCGTTCTCTGAGATAACAGATTGCTCTGATTTGCAGTCCTG   1062       Arg6Term   AAATCTAACATATC   A   GTCCTCTCTTTCCATCATTATCTCCACCAGG           AGA-TGA   CGAACAGTTAGCAGCTAAGAGTGGTAGAT               GAGAGGAC   T   GATATGTT   1063           AACATATC   A   GTCCTCTC   1064               Lead Tolerance   CCACTCTTAGCTGCTAACTGTTCGCCTGGTGGAGATAATGATGGA   1065       calmodulin binding   AAGAGAGGACAGATA   G   GTTAGATTTCAGGAGTGCAAATCAGAGCA           transport protein   ATCTGTTATCTCAGAGAACGCAGTTTCACCA             Hordeum vulgare     TGGTGAAACTGCGTTCTCTGAGATAACAGATTGCTCTGATTTGCA   1066       Tyr7Term   GTCCTGAAATCTAAC   C   TATCTGTCCTCTCTTTCCATCATTATCTCCA           TAT-TAG   CCAGGCGAACAGTTAGCAGCTAAGAGTGG               GACAGATA   G   GTTAGATT   1067           AATCTAAC   C   TATCTGTC   1068               2,4-DB resistance   ATCCTTCTCTGAGAAAAAACAACAGATCCGAATTTTATCTTTAATCA   1069       3-ketoacyl-CoA   GCCGGAAAAAATG   T   AGAAAGCGATCGAGAGACAACGCGTTCTTCT           thiolase   TGAGCATCTCCGACCTTCTTCTTCTTCTT             Arabidopsis thaliana     AAGAAGAAGAAGAAGGTCGGAGATGCTCAAGAAGAACGCGTTGT   1070       Glu2Term   CTCTCGATCGCTTTCT   A   CATTTTTTCCGGCTGATTAAAGATAAAATT           GAG-TAG   CGGATCTGTTGTTTTTTCTCAGAGAAGGAT               AAAAAATG   T   AGAAAGCG   1071           CGCTTTCT   A   CATTTTTT   1072               2,4-DB resistance   CTTCTCTGAGAAAAAACAACAGATCCGAATTTTATCTTTAATCAGC   1073       3-ketoacyl-CoA   CGGAAAAAATGGAG   T   AAGCGATCGAGAGACAACGCGTTCTTCTTG           thiolase   AGCATCTCCGACCTTCTTCTTCTTCTTCGC             Arabidopsis thaliana     GCGAAGAAGAAGAAGAAGGTCGGAGATGCTCAAGAAGAACGCGT   1074       Lys3Term   TGTCTCTCGATCGCTT   A   CTCCATTTTTTCCGGCTGATTAAAGATAA           AAA-TAA   AATTCGGATCTGTTGTTTTTTCTCAGAGAAG               AAATGGAG   T   AAGCGATC   1075           GATCGCTT   A   CTCCATTT   1076               2,4-DB resistance   GAAAAAACAACAGATCCGAATTTTATCTTTAATCAGCCGGAAAAAA   1077       3-ketoacyl-CoA   TGGAGAAAGCGATC   T   AGAGACAACGCGTTCTTCTTGAGCATCTCC           thiolase   GACCTTCTTCTTCTTCTTCGCACAATTACG             Arabidopsis thaliana     CGTAATTGTGCGAAGAAGAAGAAGAAGGTCGGAGATGCTCAAGA   1078       Glu6Term   AGAACGCGTTGTCTCT   A   GATCGCTTTCTCCATTTTTTCCGGCTGAT           GAG-TAG   TAAAGATAAAATTCGGATCTGTTGTTTTTTC               AAGCGATC   T   AGAGACAA   1079           TTGTCTCT   A   GATCGCTT   1080               2,4-DB resistance   AAAACAACAGATCCGAATTTTATCTTTAATCAGCCGGAAAAAATGG   1081       3-ketoacyl-CoA   AGAAAGCGATCGAG   T   GACAACGCGTTCTTCTTGAGCATCTCCGAC           thiolase   CTTCTTCTTCTTCTTCGCACAATTACGAGG             Arabidopsis thaliana     CCTCGTAATTGTGGGAAGAAGAAGAAGAAGGTCGGAGATGCTCAA   1082       Arg7Term   GAAGAACGCGTTGTC   A   CTCGATCGCTTTCTCCATTTTTTCCGGCT           AGA-TGA   GATTAAAGATAAAATTCGGATCTGTTGTTTT               CGATCGAG   T   GACAACGC   1083           GCGTTGTC   A   CTCGATCG   1084               2,4-DB resistance   ACAACAGATCCGAATTTTATCTTTAATCAGCCGGAAAAAATGGAGA   1085       3-ketoacyl-CoA   AAGCGATCGAGAGA   T   AACGCGTTCTTCTTGAGCATCTCCGACCTT           thiolase   CTTCTTCTTCTTCGCACAATTACGAGGCTT             Arabidopsis thaliana     AAGCCTCGTAATTGTGCGAAGAAGAAGAAGAAGGTCGGAGATGC   1086       Gln8Term   TCAAGAAGAACGCGTT   A   TCTCTCGATCGCTTTCTCCATTTTTTCCG           CAA-TAA   GCTGATTAAAGATAAAATTCGGATCTGTTGT               TCGAGAGA   T   AACGCGTT   1087           AACGCGTT   A   TCTCTCGA   1088               2,4-DB resistance   GAGAGACAAAGAGTTCTTCTTGAACATCTCCGTCCTTCTTCTTCTT   1089       glyoxysomal beta-   CCTCTCACAGCTTT   T   AAGGCTCTCTCTCTGCTTCAGCTTGCTTGGC           ketoacyol-thiolase   TGGGGACAGTGCTGCGTATCAGAGGACCT           precursor   AGGTCGTCTGATACGCAGCACTGTCCCCAGCCAAGCAAGCTGAA   1090         Brassica napus     GCAGAGAGAGAGCCTT   A   AAAGCTGTGAGAGGAAGAAGAAGAAGG           Glu26Term   ACGGAGATGTTCAAGAAGAACTCTTTGTCTCTC           GAA-TAA   ACAGCTTT   T   AAGGCTCT   1091           AGAGCCTT   A   AAAGCTGT   1092               2,4-DB resistance   TTGAACATCTCCGTCCTTCTTCTTCTTCCTCTCACAGCTTTGAAGG   1093       glyoxysomal beta-   CTCTCTCTCTGCTT   G   AGCTTGCTTGGCTGGGGACAGTGCTGCGTA           ketoacyol-thiolase   TCAGAGGACCTCTCTCTATGGAGATGATGT           precursor   ACATCATCTCCATAGAGAGAGGTCCTCTGATACGCAGCACTGTCC   1094         Brassica napus     CCAGCCAAGCAAGCT   C   AAGCAGAGAGAGAGCCTTCAAAGCTGTG           Ser32Term   AGAGGAAGAAGAAGAAGGACGGAGATGTTCAA           TCA-TGA   CTCTGCTT   G   AGCTTGCT   1095           AGCAAGCT   C   AAGCAGAG   1096               2,4-DB resistance   TCTCCGTCCTTCTTCTTCTTCCTCTCACAGCTTTGAAGGCTCTCTC   1097       glyoxysomal beta-   TCTGCTTCAGCTTG   A   TTGGCTGGGGACAGTGCTGCGTATCAGAG           ketoacyol-thiolase   GACCTCTCTCTATGGAGATGATGTAGTCATT           precursor   AATGACTACATCATCTCCATAGAGAGAGGTCCTCTGATACGCAGC   1098         Brassica napus     ACTGTCCCCAGCCAA   T   CAAGCTGAAGCAGAGAGAGAGCCTTCAAA           Cys34Term   GCTGTGAGAGGAAGAAGAAGAAGGACGGAGA           TGC-TGA   TCAGCTTG   A   TTGGCTGG   1099           CCAGCCAA   T   CAAGCTGA   1100               2,4-DB resistance   TCCGTCCTTCTTCTTGTTCCTCTCACAGCTTTGAAGGCTCTCTCTC   1101       glyoxysomal beta-   TGCTTCAGCTTGCT   A   GGCTGGGGACAGTGCTGCGTATCAGAGGA           ketoacyol-thiolase   CCTCTCTCTATGGAGATGATGTAGTCATTGT           precursor   ACAATGACTACATCATCTCCATAGAGAGAGGTCGTCTGATACGCA   1102         Brassica napus     GCACTGTCCCCAGCC   T   AGCAAGCTGAAGCAGAGAGAGAGCCTTC           Leu35Term   AAAGCTGTGAGAGGAAGAAGAAGAAGGACGGA           TTG-TAG   AGCTTGCT   A   GGCTGGGG   1103           CCCCAGCC   T   AGCAAGCT   1104               2,4-DB resistance   TCACAGCTTTGAAGGCTCTCTCTCTGCTTCAGCTTGCTTGGCTGG   1105       glyoxysomal beta-   GGACAGTGCTGCGTA   G   CAGAGGACCTCTCTCTATGGAGATGATGT           ketoacyol-thiolase   AGTCATTGTTGCGGCACATAGGACTGCACTA           precursor   TAGTGCAGTCCTATGTGCCGCAACAATGACTACATCATCTCCATA   1106         Brassica napus     GAGAGAGGTCGTCTG   C   TACGCAGCACTGTCCCCAGCCAAGCAAG           Tyr42Term   CTGAAGCAGAGAGAGAGCCTTCAAAGCTGTGA           TAT-TAG   GCTGCGTA   G   CAGAGGAC   1107           GTCCTCTG   C   TACGCAGC   1108               2,4-DB resistance   CAACAGACAGGAAGTGTTGCTCCAGCATCTCCGCCCTTCTAATTC   1109       3-ketoacyl-CoA   TTCTTCTCACAATTA   G ee GAGTCCGCTCTTGCCGCATCAGTATGTGCT             thiolase B   GCAGGGGATAGCGCCGCATATCATAGGGCT             Mangifera indica     AGCCCTATGATATGCGGCGCTATCCCCTGCAGCACATACTGATGC   1110       Tyr25Term   GGCAAGAGCGGACTC   C   TAATTGTGAGAAGAAGAATTAGAAGGGC           TAC-TAG   GGAGATGCTGGAGCAACACTTGCTGTCTGTTG               CACAATTA   G   GAGTCCGC   1111           GCGGACTC   C   TAATTGTG   1112               2,4-DB resistance   AACAGACAGCAAGTGTTGCTCCAGCATCTCCGCCCTTCTAATTCTT   1113       3-ketoacyol-CoA   CTTCTCACAATTAC   T   AGTCCGCTCTTGCCGCATCAGTATGTGCTGC           thiolase B   AGGGGATAGCGCCGCATATCATAGGGCTT             Magnifera indica     AAGCCCTATGATATGCGGCGCTATCCCCTGCAGCACATACTGATG   1114       Glu26Term   CGGCAAGAGCGGACT   A   GTAATTGTGAGAAGAAGAATTAGAAGGG           GAG-TAG   CGGAGATGCTGGAGCAACACTTGCTGTCTGTT               ACAATTAC   T   AGTCCGCT   1115           AGCGGACT   A   GTAATTGT   1116               2,4-DB resistance   TCCAGCATCTCCGCCCTTCTAATTCTTCTTCTCACAATTACGAGTC   1117       3-ketoacy\to-CoA   CGCTCTTGCCGCAT   G   AGTATGTGCTGCAGGGGATAGCGCCGCAT           thioblase B   ATCATAGGGCTTCTGTTTATGGAGACGATGT             Mangifera indica     ACATCGTCTCCATAAACAGAAGCCCTATGATATGCGGCGCTATCC   1118       Ser32Term   CCTGCAGCACATACT   C   ATGCGGCAAGAGCGGACTCGTAATTGTGA           TCA-TGA   GAAGAAGAATTAGAAGGGCGGAGATGCTGGA               TGCCGCAT   G   AGTATGTG   1119           CACATACT   C   ATGCGGCA   1120               2,4-DB resistance   TCTCCGCCCTTCTAATTCTTCTTCTCACAATTACGAGTCCGCTCTT   1121       3-ketoacyl-CoA   GCCGCATCAGTATG   A   GCTGCAGGGGATAGCGCCGGATATCATAG           thiolase B   GGCTTCTGTTTATGGAGACGATGTGGTGATT             Mangifera indica     AATCACCACATCGTCTCCATAAACAGAAGCCCTATGATATGCGGC   1122       Cys34Term   GCTATCCCCTGCAGC   T   CATACTGATGCGGCAAGAGCGGACTCGT           TGT-TGA   AATTGTGAGAAGAAGAATTAGAAGGGCGGAGA               TCAGTATG   A   GCTGCAGG   1123           CCTGCAGC   T   CATACTGA   1124               2,4-DB resistance   TCACAATTACGAGTCCGCTCTTGCCGCATCAGTATGTGCTGCAGG   1125       3-ketoacyl-CoA   GGATAGCGCCGCATA   G   CATAGGGCTTGTGTTTATGGAGACGATGT           thiolase B   GGTGATTGTGGCAGGTCATCGTACTGCACTT             Mangifera indica     AAGTGCAGTAGGATGAGCTGCCACAATCACCACATCGTCTCCATA   1126       Tyr42Term   AACAGAAGCCCTATG   C   TATGCGGCGCTATCCCCTGCAGCACATAC           TAT-TAG   TGATGCGGCAAGAGCGGACTCGTAATTGTGA               GCCGCATA   G   CATAGGGC   1127           GCCCTATG   C   TATGCGGC   1128               2,4-DB resistance   GAAGGCGATCAACAGGCAGAGCATTTTGCTACATCATCTCCGGCC   1129       3-ketoacyl-CoA   TTCTTCTTCCGCTTA   G   ACAAATGAATCTTCGCTCTCTGCATCGGTT           thiolase   TGTGCAGCTGGGGATAGTGCTTCGTATCAA             Cucumis sativus     TTGATACGAAGCACTATCCCCAGCTGCACAAACCGATGCAGAGAG   1130       Tyr22Term   CGAAGATTCATTTGT   C   TAAGCGGAAGAAGAAGGCCGGAGATGATG           TAG-TAG   TAGCAAAATGCTCTGGCTGTTGATCGCCTTC               TCCGCTTA   G   ACAAATGA   1131           TCATTTGT   C   TAAGCGGA   1132               2,4-DB resistance   ATCAACAGGCAGAGCATTTTGCTACATCATCTCCGGCCTTCTTCTT   1133       3-ketoacyl-CoA   CCGCTTACACAAAT   T   AATCTTCGCTCTCTGCATCGGTTTGTGCAGC           thiolase   TGGGGATAGTGCTTCGTATCAAAGGACAT             Cucumis sativus     ATGTCCTTTGATACGAAGCAGTATCCCCAGCTGCACAAACCGATG   1134       Glu25Term   CAGAGAGCGAAGATT   A   ATTTGTGTAAGCGGAAGAAGAAGGCCGG           GAA-TAA   AGATGATGTAGCAAAATGCTCTGCCTGTTGAT               ACACAAAT   T   AATCTTCG   1135           CGAAGATT   A   ATTTGTGT   1136               2,4-DB resistance   GGCAGAGCATTTTGCTACATCATCTCCGGCCTTCTTCTTCCGCTTA   1137       3-ketoacyl-CoA   CACAAATGAATCTT   A   GCTCTCTGCATCGGTTTGTGCAGCTGGGGA           thiolase   TAGTGCTTCGTATCAAAGGACATCGGTGTT             Cucumis sativus     AACACCGATGTCCTTTGATACGAAGCACTATCCCCAGCTGCACAA   1138       Ser27Term   ACCGATGCAGAGAGC   T   AAGATTCATTTGTGTAAGCGGAAGAAGAA           TCG-TAG   GGCCGGAGATGATGTAGCAAAATGCTCTGCC               TGAATCTT   A   GCTCTCTG   1139           CAGAGAGC   T   AAGATTCA   1140               2,4-DB resistance   TGCTACATCATCTCCGGCCTTCTTCTTCCGCTTACACAAATGAATC   1141       3-ketoacyl-CoA   TTCGCTCTCTGCAT   A   GGTTTGTGCAGCTGGGGATAGTGCTTCGTA           thiolase   TCAAAGGACATCGGTGTTTGGAGATGATGT             Cucumis sativus     ACATCATCTCCAAACACCGATGTCCTTTGATACGAAGCACTATCCC   1142       Ser31Term   CAGCTGCACAAACC   T   ATGCAGAGAGCGAAGATTCATTTGTGTAAG           TCG-TAG   CGGAAGAAGAAGGCCGGAGATGATGTAGCA               CTCTGCAT   A   GGTTTGTG   1143           CACAAACC   T   ATGCAGAG   1144               2,4-DB resistance   TCATCTCCGGCCTTCTTCTTCCGCTTACACAAATGAATCTTCGCTC   1145       3-ketoacyl-CoA   TCTGCATCGGTTTG   A   GCAGCTGGGGATAGTGCTTCGTATCAAAGG           thiolase   ACATCGGTGTTTGGAGATGATGTCGTGATT             Cucumis sativus     AATCACGACATCATCTCCAAACACCGATGTCCTTTGATACGAAGCA   1146       Cys33Term   CTATCCCCAGCTGC   T   CAAACCGATGCAGAGAGCGAAGATTCATTT           TGT-TGA   GTGTAAGCGGAAGAAGAAGGCCGGAGATGA               TCGGTTTG   A   GCAGCTGG   1147           CCAGCTGC   T   CAAACCGA   1148               2A-DB resistance   GAAGGCAATCAACAGGCAGAGCATTCTGCTACATCATCTCCGGCC   1149       3-ketoacyl-CoA   TTCATCTTCGGCTTA   G   ACCCATGAATCTTCGCTCTCTGCATCGGTT           thiolase   TGTGCAGCTGGGGATAGTGCGTCGTATCAA           Cucurbita sp.   TTGATACGACGCACTATCCCCAGCTGCACAAACCGATGCAGAGAG   1150       Tyr22Term   CGAAGATTCATGGCT   C   TAAGCCGAAGATGAAGGCCGGAGATGAT           TAT-TAG   GTAGCAGAATGCTCTGCCTGTTGATTGCCTTC               TCGGCTTA   G   AGCCATGA   1151           TCATGGCT   C   TAAGCCGA   1152               2,4-DB resistance   ATCAACAGGCAGAGCATTCTGCTACATCATCTCCGGCCTTCATCTT   1153       3-ketoacyl-CoA   CGGCTTATAGCCAT   T   AATCTTCGCTCTCTGCATCGGTTTGTGCAGC           thiolase   TGGGGATAGTGCGTCGTATCAAAGAACGT           Cucurbita sp.   ACGTTCTTTGATACGACGCACTATCCCCAGCTGCACAAACCGATG   1154       Glu25Term   CAGAGAGCGAAGATT   A   ATGGCTATAAGCCGAAGATGAAGGCCGG           GAA-TAA   AGATGATGTAGCAGAATGCTCTGCCTGTTGAT               ATAGCCAT   T   AATCTTCG   1155           CGAAGATT   A   ATGGCTAT   1156               2,4-DB resistance   GGCAGAGCATTCTGCTACATCATCTCCGGCCTTCATCTTCGGCTT   1157       3-ketoacyl-CoA   ATAGCCATGAATCTT   A   GCTCTCTGCATCGGTTTGTGCAGCTGGGG           thiolase   ATAGTGCGTCGTATCAAAGAACGTCGGTGTT           Cucurbita sp.   AACACCGACGTTCTTTGATACGACGCACTATCCCCAGCTGCACAA   1158       Ser27Term   ACCGATGCAGAGAGCTAAGATTCATGGCTATAAGCCGAAGATGAA           TCG-TAG   GGCCGGAGATGATGTAGCAGAATGCTCTGCC               TGAATCTT   A   GCTCTCTG   1159           CAGAGAGC   T   AAGATTCA   1160               2,4-DB resistance   TGCTACATCATCTCCGGCCTTCATCTTCGGCTTATAGCCATGAATC   1161       3-ketoacyl-CoA   TTCGCTCTCTGCAT   A   GGTTTGTGCAGCTGGGGATAGTGCGTCGTA           thiolase   TCAAAGAACGTCGGTGTTTGGAGATGATGT           Cucurbita sp.   ACATCATCTCCAAACACCGACGTTCTTTGATACGACGCACTATCCC   1162       Ser31Term   CAGCTGCACAAACC   T   ATGCAGAGAGCGAAGATTCATGGCTATAAG           TCG-TAG   CCGAAGATGAAGGCCGGAGATGATGTAGCA               CTCTGCAT   A   GGTTTGTG   1163           CACAAACC   T   ATGCAGAG   1164               2,4-DB resistance   TCATCTCCGGCCTTCATCTTCGGCTTATAGCCATGAATCTTCGCTC   1165       3-ketoacyl-CoA   TCTGCATCGGTTTG   A   GCAGCTGGGGATAGTGCGTCGTATCAAAGA           thiolase   ACGTCGGTGTTTGGAGATGATGTCGTGATA           Cucurbita sp.   TATCACGACATCATCTCCAAACACCGACGTTCTTTGATACGACGCA   1166       Cys33Term   CTATCCCCAGCTGC   T   CAAACCGATGCAGAGAGCGAAGATTCATGG           TGT-TGA   CTATAAGCCGAAGATGAAGGCCGGAGATGA               TCGGTTTG   A   GCAGCTGG   1167           CCAGCTGC   T   CAAACCGA   1168               2,4 DB resistance   TCATAGTCTCTTTTGCCGCTTGGATTCTTCCAAGGTTAGTGAGCTG   1169       Pex14   CTATGGCAACTCAT   T   AGCAAACGCAACCTCCTTCCGATTTTCCCGC             Arabidopsis thaliana     TCTTGCCGATGAAAATTCCCAGATTCCAG           Gln5Term   CTGGAATCTGGGAATTTTCATCGGCAAGAGCGGGAAAATCGGAA   1170       CAG-TAG   GGAGGTTGCGTTTGCT   A   ATGAGTTGCCATAGCAGCTCACTAACCT               TGGAAGAATCCAAGCGGCAAAAGAGACTATGA               CAACTCAT   T   AGCAAACG   1171           CGTTTGCT   A   ATGAGTTG   1172               2,4 DB resistance   TAGTCTCTTTTGCCGCTTGGATTCTTCCAAGGTTAGTGAGCTGCTA   1173       Pex14   TGGCAACTCATCAG   T   AAACGCAACCTCCTTCCGATTTTCCCGCTCT             Arabidopsis thaliana     TGCCGATGAAAATTCCCAGATTGCAGGTT           Gln6Term   AACCTGGAATCTGGGAATTTTCATCGGCAAGAGCGGGAAAATCGG   1174       CAA-TAA   AAGGAGGTTGCGTTT   A   CTGATGAGTTGCCATAGCAGCTCACTAAC               CTTGGAAGAATCCAAGCGGCAAAAGAGACTA               CTCATCAG   T   AAACGCAA   1175           TTGCGTTT   A   CTGATGAG   1176               2,4 DB resistance   CTTTTGCCGCTTGGATTCTTCCAAGGTTAGTGAGCTGCTATGGCA   1177       Pex14   ACTCATCAGCAAACG   T   AACCTCCTTCCGATTTTCCCGCTCTTGCCG             Arabidopsis thaliana     ATGAAAATTCCGAGATTCCAGGTTCAATTT           Gln8Term   AAATTGAACCTGGAATCTGGGAATTTTCATCGGCAAGAGCGGGAA   1178       CAA-TAA   AATCGGAAGGAGGTT   A   CGTTTGCTGATGAGTTGCCATAGCAGCTC               ACTAACCTTGGAAGAATCCAAGCGGCAAAAG               AGCAAACG   T   AACCTCCT   1179           AGGAGGTT   A   CGTTTGCT   1180               2,4 DB resistance   GCTGCTATGGCAACTGATGAGCAAACGCAACCTCCTTCCGATTTT   1181       Pex14   CCCGCTCTTGCCGAT   T   AAAATTCCCAGATTCCAGGTTCAATTTACA             Arabidopsis thaliana     CCTTCTAATCATTATTTCTTAATTTTTCTT           Glu19Term   AAGAAAAATTAAGAAATAATGATTAGAAGGTGTAAATTGAACCTGG   1182       GAA-TAA   AATCTGGGAATTTT   A   ATCGGCAAGAGCGGGAAAATCGGAAGGAG               GTTGCGTTTGCTGATGAGTTGCCATAGCAGC               TTGCCGAT   T   AAAATTCC   1183           GGAATTTT   A   ATCGGCAA   1184               2,4 DB resistance   GCAACTCATCAGCAAACGCAACCTCCTTCCGATTTTCCCGCTCTT   1185       Pex14   GCCGATGAAAATTCC   T   AGATTCCAGGTTCAATTTACACCTTCTAAT             Arabidopsis thaliana     CATTATTTCTTAATTTTTCTTTGGTGGATT           Gln22Term   AATCCACCAAAGAAAAATTAAGAAATAATGATTAGAAGGTGTAAAT   1186       CAG-TAG   TGAACCTGGAATCT   A   GGAATTTTCATCGGCAAGAGCGGGAAAATC               GGAAGGAGGTTGCGTTTGCTGATGAGTTGC               AAAATTCC   T   AGATTCCA   1187           TGGAATCT   A   GGAATTTT   1188                  
 
     EXAMPLE 8  
     Production of Albino Mutants for the Analysis of Photosynthetic Processes  
     [0132] Plant productivity is limited by resources available and the ability of plants to harness these resources. The conversion of light to chemical energy, which is then used to synthesize carbohydrates, fatty acids, sugars, amino acids and other compounds, requires a complex system which combines the light harvesting apparatus of pigments and proteins. The value of light energy to the plant can only be realized when it is efficiently converted into chemical energy by photosynthesis and fed into various biochemical processes. Significant effort has therefore been directed at studying photosynthetic processes in plants in order to improve productivity and/or the efficiency of photosynthesis. The analysis of the photosynthetic process is substantially aided by the ability to produce albino plants.  
     [0133] The attached table discloses exemplary oligonucleotide base sequences which can be used to generate site-specific mutations in genes involved in starch metabolism.  
                   TABLE 18                          Oligonucleotides to produce albino plants                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               White leaves   TTCTTTCCTGTGAAATTATCTGCTCAAATCTTTGGTTCCTGACGGAG   1189           Immutans   ATGGCGGCGATTT   G   AGGCATCTCCTCTGGTACGTTGACGATTTCA         Arabidopsis thaliana     CGGCCTTTGGTTACTCTTCGACGCTCTAG       Ser5Term   CTAGAGCGTCGAAGAGTAACCAAAGGCCGTGAAATCGTCAACGTA   1190       TCA-TGA   CCAGAGGAGATGCCT   C   AAATCGCCGCCATCTCCGTCAGGAACCAA           AGATTTGAGCAGATAATTTCACAGGAAAGAA           GGCGATTT   G   AGGCATCT   1191           AGATGCCT   C   AAATCGCC   1192               White leaves   GCTCAAATCTTTGGTTCCTGACGGAGATGGCGGCGATTTCAGGCA   1193       Immutans   TCTCCTCTGGTACGT   A   GACGATTTCACGGCCTTTGGTTACTCTTCG         Arabidopsis thaliana     ACGCTCTAGAGCCGCCGTTTCGTACAGCTC       Leu12Term   GAGCTGTACGAAACGGCGGCTCTAGAGCGTCGAAGAGTAACCAAA 1194       TTG-TAG   GGCCGTGAAATCGTC   T   ACGTACCAGAGGAGATGCCTGAAATCGCC           GCCATCTCCGTCAGGAACCAAAGATTTGAGC           TGGTACGT   A   GACGATTT   1195           AAATCGTC   T   ACGTACCA   1196               White leaves   TTTGGTTCCTGACGGAGATGGCGGCGATTTCAGGCATCTCCTCTG   1197       Immutans   GTACGTTGACGATTTGACGGCCTTTGGTTACTCTTCGACGCTCTAG         Arabidopsis thaliana     AGCCGCCGTTTCGTACAGCTCCTCTCACCG       Ser15Term   CGGTGAGAGGAGCTGTACGAAACGGCGGCTCTAGAGCGTCGAAG   1198       TCA-TGA   AGTAACCAAAGGCCGT   C   AAATCGTCAACGTACCAGAGGAGATGCC           TGAAATCGCCGCCATCTCCGTCAGGAACCAAA           GACGATTT   G   ACGGCCTT   1199           AAGGCCGT   C   AAATCGTC   1200               White leaves   GCGGCGATTTCAGGCATCTCCTCTGGTACGTTGACGATTTCACGG   1201       Immutans   CCTTTGGTTACTCTT   T   GACGCTCTAGAGCCGCCGTTTCGTACAGCT         Arabidopsis thaliana     CCTCTCACCGATTGCTTCATCATCTTCCTC       Arg22Term   GAGGAAGATGATGAAGCAATCGGTGAGAGGAGCTGTACGAAACG   1202       CGA-TGA   GCGGCTCTAGAGCGTC   A   AAGAGTAACCAAAGGCCGTGAAATCGTC           AACGTACCAGAGGAGATGCCTGAAATCGCCGC           TTACTCTT   T   GACGCTCT   1203           AGAGCGTC   A   AAGAGTAA   1204               White leaves   TCAGGCATCTCCTCTGGTACGTTGACGATTTCACGGCCTTTGGTTA   1205       Immutans   CTCTTCGACGCTCT   T   GAGCCGCCGTTTCGTACAGCTCCTCTCACC         Arabidopsis thaliana     GATTGCTTCATCATCTTCCTCTCTCTTCTC       Arg25Term   GAGAAGAGAGAGGAAGATGATGAAGCAATCGGTGAGAGGAGCTG   1206       AGA-TGA   TACGAAACGGCGGCTC   A   AGAGCGTCGAAGAGTAACCAAAGGCCG           TGAAATCGTCAACGTACCAGAGGAGATGCCTGA           GACGCTCT   T   GAGCCGCC   1207           GGCGGCTC   A   AGAGCGTC   1208               White leaves   GATTCTTGTGGGAAGGAAGAAGGATCAAGAATGGCGATTTCGATT   1209       Immutans   TCTGCTATGAGTTTT   T   GAACCTCAGTTTCTTCATATTCTTGTTTTAG         Lycopersicon     AGCTAGGAGTTTTGAGAAGTCATCAGTTT         esculentum     AAACTGATGACTTCTCAAAACTCCTAGCTCTAAAACAAGAATATGA   1210       Gly11Term   AGAAACTGAGGTTC   A   AAAACTCATAGCAGAAATCGAAATCGCCATT       GGA-TGA   CTTGATCCTTCTTCCTTCCCACAAGAATC           TGAGTTTT   T   GAACCTCA   1211           TGAGGTTC   A   AAAACTCA   1212               White leaves   GTGGGAAGGAAGAAGGATCAAGAATGGCGATTTCGATTTCTGCTA   1213       Immutans   TGAGTTTTGGAACCT   G   AGTTTCTTCATATTCTTGTTTTAGAGCTAGG         Lycopersicon     AGTTTTGAGAAGTCATCAGTTTTATGCAA         esculentum     TTGCATAAAACTGATGACTTCTCAAAACTCCTAGCTCTAAAACAAG   1214       Ser13Term   AATATGAAGAAACT   C   AGGTTCCAAAACTCATAGCAGAAATCGAAAT       TCA-TGA   CGCCATTCTTGATCCTTCTTCCTTCCCAC           TGGAACCT   G   AGTTTCTT   1215           AAGAAACT   C   AGGTTCCA   1216               White leaves   AAGAAGGATCAAGAATGGCGATTTCGATTTCTGCTATGAGTTTTGG   1217       Immutans   AACCTCAGTTTCTTGATATTCTTGTTTTAGAGCTAGGAGTTTTGAGA         Lycopersicon     AGTCATCAGTTTTATGCAATTCCCAGAA         esculentum     TTCTGGGAATTGCATAAAACTGATGACTTCTCAAAACTCCTAGCTC   1218       Ser16Term   TAAAACAAGAATAT   C   AAGAAACTGAGGTTCCAAAACTCATAGCAGA       TCA-TGA   AATCGAAATCGCCATTCTTGATCCTTCTT           AGTTTCTT   G   ATATTCTT   1219           AAGAATAT   C   AAGAAACT   1220               White leaves   AGGATCAAGAATGGCGATTTCGATTTCTGCTATGAGTTTTGGAACC   1221       Immutans   TCAGTTTCTTCATA   G   TCTTGTTTTAGAGCTAGGAGTTTTGAGAAGTC         Lycopersicon     ATCAGTTTTATGCAATTCCCAGAACCCA         esculentum     TGGGTTCTGGGAATTGCATAAAACTGATGACTTCTCAAAACTCCTA   1222       Tyr17Term   GCTCTAAAACAAGA   C   TATGAAGAAACTGAGGTTCCAAAACTCATAG       TAT-TAG   CAGAAATCGAAATCGCCATTCTTGATCCT           TCTTCATA   G   TCTTGTTT   1223           AAACAAGA   C   TATGAAGA   1224               White leaves   AAGAATGGCGATTTCGATTTCTGCTATGAGTTTTGGAACCTCAGTT   1225       Immutans   TCTTCATATTCTTG   A   TTTAGAGCTAGGAGTTTTGAGAAGTCATCAGT         Lycopersicon     TTTATGCAATTCCCAGAACCCATGTCGG         esculentum     CCGACATGGGTTCTGGGAATTGCATAAAACTGATGACTTCTCAAAA   1226       Cys19Term   CTCCTAGCTCTAAA   T   CAAGAATATGAAGAAACTGAGGTTCCAAAAC       TGT-TGA   TCATAGCAGAAATCGAAATCGCCATTCTT           TATTCTTG   A   TTTAGAGC   1227           GCTCTAAA   T   CAAGAATA   1228               White leaves   CGCGTCCGATAAAAAAATCAAGAATGGCGATTTCCATATCTGCTAT   1229       Immutans   GAGTTTTCGAACTT   G   AGTTTCTTCTTCATATTCAGCATTTTTGTGCA         Capsicum annuum     ATTCCAAGAACCCATTTTGTTTGAATTC       Ser13Term   GAATTCAAACAAAATGGGTTCTTGGAATTGCACAAAAATGCTGAAT   1230       TCA-TGA   ATGAAGAAGAAACT   C   AAGTTCGAAAACTCATAGCAGATATGGAAAT           CGCCATTCTTGATTTTTTTATCGGACGCG           TCGAACTT   G   AGTTTCTT   1231           AAGAAACT   C   AAGTTCGA   1232               White leaves   AAAAATCAAGAATGGCGATTTCCATATCTGCTATGAGTTTTCGAAC   1233       Immutans   TTCAGTTTCTTCTT   G   ATATTCAGCATTTTTGTGCAATTCCAAGAACC         Capsicum annuum     CATTTTGTTTGAATTCTCTATTTTCACT       Ser17Term   AGTGAAAATAGAGAATTCAAACAAAATGGGTTCTTGGAATTGCACA   1234       TCA-TGA   AAAATGCTGAATAT   C   AAGAAGAAACTGAAGTTCGAAAACTCATAGC           AGATATGGAAATCGCCATTCTTGATTTTT           TTCTTCTT   G   ATATTCAG   1235           CTGAATAT   C   AAGAAGAA   1236               White leaves   CAAGAATGGCGATTTCCATATCTGCTATGAGTTTTCGAACTTCAGT   1237       Immutans   TTCTTCTTCATATT   G   AGCATTTTTGTGCAATTCCAAGAACCCATTTT         Capsicum annuum     GTTTGAATTCTCTATTTTCACTTAGGAA       Ser19Term   TTCCTAAGTGAAAATAGAGAATTCAAACAAAATGGGTTCTTGGAAT   1238       TCA-TGA   TGCACAAAAATGCT   C   AATATGAAGAAGAAACTGAAGTTCGAAAACT           CATAGCAGATATGGAAATCGCCATTCTTG           TTCATATT   G   AGCATTTT   1239           AAAATGCT   C   AATATGAA   1240               White leaves   CGATTTCCATATCTGCTATGAGTTTTCGAACTTCAGTTTCTTCTTCA   1241       Immutans   TATTCAGCATTTT   A   GTGCAATTCCAAGAACCCATTTTGTTTGAATTC         Capsicum annuum     TCTATTTTCACTTAGGAATTCTCATAG       Leu21Term   CTATGAGAATTCCTAAGTGAAAATAGAGAATTCAAACAAAATGGGT   1242       TTG-TAG   TCTTGGAATTGCAC   T   AAAATGCTGAATATGAAGAAGAAACTGAAGT           TCGAAAACTCATAGCAGATATGGAAATCG           AGCATTTT   A   GTGCAATT   1243           AATTGCAC   T   AAAATGCT   1244               White leaves   TTCCATATCTGCTATGAGTTTTCGAACTTCAGTTTCTTCTTCATATT   1245       Immutans   CAGCATTTTTGTG   A   AATTCCAAGAACCCATTTTGTTTGAATTCTCTA         Capsicum annuum     TTTTCACTTAGGAATTCTCATAGAACT       Cys22Term   AGTTCTATGAGAATTCCTAAGTGAAAATAGAGAATTCAAACAAAAT   1246       TGC-TGA   GGGTTCTTGGAATT   T   CACAAAAATGCTGAATATGAAGAAGAAACTG           AAGTTCGAAAACTCATAGCAGATATGGAA           TTTTTGTG   A   AATTCCAA   1247           TTGGAATT   T   CACAAAAA   1248               White leaves   TTCGGCACGAGGGAGAAGGAGCAGACCGAGGTGGCCGTCGAGG   1249       Immutans   AGTCCTTCCCCTTCAGG   T   AGACGGCTCCTCCTGACGAGCCACTGG         Oryza sativa     TCACCGCCGAGGAGAGCTGGGTGGTTAAGCTCG       Glu22Term   CGAGCTTAACCACCCAGCTCTCCTCGGCGGTGACCAGTGGCTCGT   1250       GAG-TAG   CAGGAGGAGCCGTCT   A   CCTGAAGGGGAAGGACTCCTCGACGGCC           ACCTCGGTCTGCTCCTTCTCCCTCGTGCCGAA           CCTTCAGG   T   AGACGGCT   1251           AGCCGTCT   A   CCTGAAGG   1252               White leaves   GAGCAGACCGAGGTGGCCGTCGAGGAGTCCTTCCCCTTCAGGGA   1253       Immutans   GACGGCTCCTCCTGAC   T   AGCCACTGGTCACCGCCGAGGAGAGCT         Oryza sativa     GGGTGGTTAAGCTCGAGCAGTCCGTGAACATTT       Glu28Term   AAATGTTCACGGACTGCTCGAGCTTAACCACCCAGCTCTCCTCGG   1254       CAG-TAG   CGGTGACCAGTGGCT   A   GTCAGGAGGAGCCGTCTCCCTGAAGGGG           AAGGACTCCTCGACGGCCACCTCGGTCTGCTC           CTCCTGAC   T   AGCCACTG   1255           CAGTGGCT   A   GTCAGGAG   1256               White leaves   GTCGAGGAGTCCTTCCCCTTCAGGGAGACGGCTCCTCCTGACGA   1257       Immutans   GCCACTGGTCACCGCC   T   AGGAGAGCTGGGTGGTTAAGCTCGAGC         Oryza sativa     AGTCCGTGAACATTTTCCTCACGGAGTCAGTCA       Glu34Term   TGACTGACTCCGTGAGGAAAATGTTCACGGACTGCTCGAGCTTAA   1258       GAG-TAG   CCACCCAGCTCTCCTAGGCGGTGACCAGTGGCTCGTCAGGAGGA           GCCGTCTCCCTGAAGGGGAAGGACTCCTCGAC           TCACCGCC   T   AGGAGAGC   1259           GCTCTCCT   A   GGCGGTGA   1260               White leaves   GAGGAGTCCTTCCCCTTCAGGGAGACGGCTCCTCCTGACGAGCC   1261       Immutans   ACTGGTCACCGCCGAG   T   AGAGCTGGGTGGTTAAGCTCGAGCAGT         Oryza sativa     CCGTGAACATTTTCCTCACGGAGTCAGTCATCA       Glu35Term   TGATGACTGACTCCGTGAGGAAAATGTTCACGGACTGCTCGAGCT   1262       GAG-TAG   TAACCACCCAGCTCT   A   CTCGGCGGTGACCAGTGGCTCGTCAGGA           GGAGCCGTCTCCCTGAAGGGGAAGGACTCCTC           CCGCCGAG   T   AGAGCTGG   1263           CCAGCTCT   A   CTCGGCGG   1264               White leaves   CTTCCCCTTCAGGGAGACGGCTCCTCCTGACGAGCCACTGGTCAC   1265       Immutans   CGCCGAGGAGAGCTG   A   GTGGTTAAGCTCGAGCAGTCCGTGAACA         Oryza sativa     TTTTCCTCACGGAGTCAGTCATCACGATACTT       Trp37Term   AAGTATCGTGATGACTGACTCCGTGAGGAAAATGTTCACGGACTG   1266       TGG-TGA   CTCGAGCTTAACCAC   T   CAGCTCTCCTCGGCGGTGACCAGTGGCTC           GTCAGGAGGAGCCGTCTCCCTGAAGGGGAAG           GAGAGCTG   A   GTGGTTAA   1267           TTAACCAC   T   CAGCTCTC   1268               White leaves   TCCGGAGGAGGAAGGGGGATTCGACGAGGAGCTCACCCTCGCCG   1269       Immutans   GCGAGGACGGCGACTGAGTCGTCAGATTCGAGCAGTCCTTCAAC         Triticum aestivum     GTATTCCTCACGGATACTGTCATCTTTATACTC       Trp22Term   GAGTATAAAGATGACAGTATCCGTGAGGAATACGTTGAAGGACTG   1270       TGG-TGA   CTCGAATCTGACGAC   T   CAGTCGCCGTCCTCGCCGGCGAGGGTGA           GCTCCTCGTCGAATCCCCCTTCCTCCTCCGGA           GGCGACTG   A   GTCGTCAG   1271           CTGACGAC   T   CAGTCGCC   1272               White leaves   GAGGAAGGGGGATTCGACGAGGAGCTCACCCTCGCCGGCGAGG   1273       Immutans   ACGGCGACTGGGTCGTC   T   GATTCGAGCAGTCCTTCAACGTATTCC         Triticum aestivum     TCACGGATACTGTCATCTTTATACTCGATATTC       Arg25Term   GAATATCGAGTATAAAGATGACAGTATCCGTGAGGAATACGTTGAA   1274       AGA-TGA   GGACTGCTCGAATC   A   GACGACCCAGTCGCCGTCCTCGCCGGCGA           GGGTGAGCTCCTCGTCGAATCCCCCTTCCTC           GGGTCGTC   T   GATTCGAG   1275           CTCGAATC   A   GACGACCC   1276               White leaves   GGGGGATTCGACGAGGAGCTCACCCTCGCCGGCGAGGACGGCG   1277       Immutans   ACTGGGTCGTCAGATTCTAGCAGTCCTTCAACGTATTCCTCACGGA         Triticum aestivum     TACTGTCATCTTTATACTCGATATTCTGTATC       Glu21Term   GATACAGAATATCGAGTATAAAGATGACAGTATCCGTGAGGAATAC   1278       GAG-TAG   GTTGAAGGACTGCT   A   GAATCTGACGACCCAGTCGCCGTCCTCGCC           GGCGAGGGTGAGCTCCTCGTCGAATCCCCC           TCAGATTC   T   AGCAGTCC   1279           GGACTGCT   A   GAATCTGA   1280               White leaves   GGATTCGACGAGGAGCTCACCCTCGCCGGCGAGGACGGCGACTG   1281       Immutans   GGTCGTCAGATTCGAG   T   AGTCCTTCAACGTATTCCTCACGGATACT         Triticum aestivum     GTCATCTTTATACTCGATATTCTGTATCGTG       Gln28Term   CACGATACAGAATATCGAGTATAAAGATGACAGTATCCGTGAGGAA   1282       CAG-TAG   TACGTTGAAGGACTACTCGAATCTGACGACCCAGTCGCCGTCCTC           GCCGGCGAGGGTGAGCTCCTCGTCGAATCC           GATTCGAG   T   AGTCCTTC   1283           GAAGGACT   A   CTCGAATC   1284               White leaves   CGAGCAGTCCTTCAACGTATTCCTCACGGATACTGTCATCTTTATA   1285       Immutans   CTCGATATTCTGTA   G   CGTGACCGCGACTACGCAAGGTTCTTCGTG         Triticum aestivum     CTCGAGACCATCGCCAGGGTGCCCTATTTC       Tyr46Term   GAAATAGGGCACCCTGGCGATGGTCTCGAGCACGAAGAACCTTG   1286       TAT-TAG   CGTAGTCGCGGTCACGCTACAGAATATCGAGTATAAAGATGACAG           TATCCGTGAGGAATACGTTGAAGGACTGCTCG           ATTCTGTA   G   CGTGACCG   1287           CGGTCACG   C   TACAGAAT   1288                  
 
     EXAMPLE 9  
     Altering Amino Acid Content of Plants  
     [0134] Another aim of biotechnology is to generate plants, especially crop plants, with added value traits. An example of such a trait is improved nutritional quality in food crops. For example, lysine, tryptophan and threonine, which are essential amino acids in the diet of humans and many animals, are limiting nutrients in most cereal crops. Consequently, grain-based diets, such as those based on corn, barley, wheat, rice, maize, millet, sorghum, and the like, must be supplemented with more expensive synthetic amino acids or amino-acid-containing oilseed protein meals. Increasing the lysine content of these grains or of any of the feed component crops would result in significant added value.  
     [0135] Naturally occurring mutants of plants that have different levels of particular essential amino acids have been identified. However, these mutants are generally not the result of increased free amino acid, but are instead the result of shifts in the overall protein profile of the grain. For example, in maize, reduced levels of lysine-deficient endosperm proteins (prolamines) are complemented by elevated levels of more lysine-rich proteins (albumins, globulins and glutelins). While nutritionally superior, these mutants are associated with reduced yields and poor grain quality, limiting their agronomic usefulness.  
     [0136] An alternative approach is to generate plants with mutations that render key amino acid biosynthetic enzymes insensitive to feedback inhibition. Many such mutations are known and mutation results in increased free amino acid. The increased production can optionally be coupled to increased expression of an abundant storage protein comprising the chosen amino acid. Alternatively, a normally abundant protein can be engineered to contain more of the target amino acid.  
     [0137] The attached table discloses exemplary oligonucleotide base sequences which can be used to generate site-specific mutations that remove feedback inhibition in plant amino acid biosynthetic enzymes.  
                   TABLE 19                          Genome-Altering Oligos Conferring Amino Acid Overproduction                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               Met Overproduction   TATCCTCCAGGATCTTAAGATTTCCTCCTAATTTCGTCCGTCAGCT   1289           CGS   GAGCATTAAAGCCC   A   TAGAAACTGTAGCAACATCGGTGTTGCACA         Arabidopsis thaliana     GATCGTGGCGGCTAAGTGGTCCAACAACCC       Arg77His   GGGTTGTTGGACCACTTAGCCGCCACGATCTGTGCAACACCGAT   1290       CGT-CAT   GTTGCTACAGTTTCTA   T   GGGCTTTAATGCTCAGCTGACGGACGAA           ATTAGGAGGAAATCTTAAGATCCTGGAGGATA           TAAAGCCC   A   TAGAAACT   1291           AGTTTCTA   T   GGGCTTTA   1292               Met Overproduction   TCTTAAGATTTCCTCCTAATTTCGTCCGTCAGCTGAGCATTAAAGC   1293       CGS   CCGTAGAAACTGTA   A   CAACATCGGTGTTGCACAGATCGTGGCGG         Arabidopsis thaliana     CTAAGTGGTCCAACAACCCATCCTCCGCGTT       Ser81Asn   AACGCGGAGGATGGGTTGTTGGACCACTTAGCCGCCACGATCTG   1294       AGC-AAC   TGCAACACCGATGTTG   T   TACAGTTTCTACGGGCTTTAATGCTCAGC           TGACGGACGAAATTAGGAGGAAATCTTAAGA           AAACTGTA   A   CAACATCG   1295           CGATGTTG   T   TACAGTTT   1296               Met Overproduction   TTTCCTCCTAATTTCGTCCGTCAGCTGAGCATTAAAGCCCGTAGAA   1297       CGS   ACTGTAGCAACATC   A   GTGTTGCACAGATCGTGGCGGCTAAGTGGT         Arabidopsis thaliana     CCAACAACCCATCCTCCGCGTTACCTTCGG       Gly84Ser   CCGAAGGTAACGCGGAGGATGGGTTGTTGGACCACTTAGCCGCC   1298       GGT-AGT   ACGATCTGTGCAACAC   T   GATGTTGCTACAGTTTCTACGGGCTTTAA           TGCTCAGCTGACGGACGAAATTAGGAGGAAA           GCAACATC   A   GTGTTGCA   1299           TGCAACAC   T   GATGTTGC   1300               Met Overproduction   TTCCTCCTAATTTCGTCCGTCAGCTGAGCATTAAAGCCCGTAGAAA   1301       CGS   CTGTAGCAACATCG   A   TGTTGCACAGATCGTGGCGGCTAAGTGGTC         Arabidopsis thaliana     CAACAACCCATCCTCCGCGTTACCTTCGGC       Gly84Asp   GCCGAAGGTAACGCGGAGGATGGGTTGTTGGACCACTTAGCCGC   1302       GGT-GAT   CACGATCTGTGCAACA   T   CGATGTTGCTACAGTTTCTACGGGCTTTA           ATGCTCAGCTGACGGACGAAATTAGGAGGAA           CAACATCG   A   TGTTGCAC   1303           GTGCAACA   T   CGATGTTG   1304               Met Overproduction   TATCGTCACTCATCCTCCGCTTCCCTCCCAACTTCGTCCGCCAGC   1305       CGS   TCAGCACCAAGGCCC   A   CCGCAACTGCAGCAACATCGGCGTCGCG         Fragraria vesca     CAGATCGTCGCGGCTTCGTGGTCCAACAAAGA       Arg73His   TCTTTGTTGGACCACGAAGCCGCGACGATCTGCGCGACGCCGAT   1306       CGC-CAC   GTTGCTGCAGTTGCGG   T   GGGCCTTGGTGCTGAGCTGGCGGACGA           AGTTGGGAGGGAAGCGGAGGATGAGTGACGATA           CAAGGCCC   A   CCGCAACT   1307           AGTTGCGG   T   GGGCCTTG   1308               Met Overproduction   TCCTCCGCTTCCCTCCCAACTTCGTCCGCCAGCTCAGCACCAAGG   1309       CGS   CCCGCCGCAACTGCA   A   CAACATCGGCGTCGCGCAGATCGTCGCG         Fragraria vesca     GCTTCGTGGTCCAACAAAGACTCCGACCTTTC       Ser77Asn   GAAAGGTCGGAGTCTTTGTTGGACCACGAAGCCGCGACGATCTG   1310       AGC-AAC   CGCGACGCCGATGTTG   T   TGCAGTTGCGGCGGGCCTTGGTGCTGA           GCTGGCGGACGAAGTTGGGAGGGAAGCGGAGGA           CAACTGCA   A   CAACATCG   1311           CGATGTTG   T   TGCAGTTG   1312               Met Overproduction   TTCCCTCCCAACTTCGTCCGCCAGCTCAGCACCAAGGCCCGCCG   1313       CGS   CAACTGCAGCAACATC   A   GCGTCGCGCAGATCGTCGCGGCTTCGT         Fragraria vesca     GGTCCAACAAAGACTCCGACCTTTCGGCGGTGC       Gly80Ser   GCACCGCCGAAAGGTCGGAGTCTTTGTTGGACCACGAAGCCGCG   1314       GGC-AGC   ACGATCTGCGCGACGC   T   GATGTTGGTGCAGTTGCGGCGGGCCTT           GGTGCTGAGCTGGCGGACGAAGTTGGGAGGGAA           GCAACATC   A   GCGTCGCG   1315           CGCGACGC   T   GATGTTGC   1316               Met Overproduction   TCCCTCCCAACTTCGTCCGCCAGCTCAGCACCAAGGCCCGCCGC   1317       CGS   AACTGCAGCAACATCG   A   CGTCGCGCAGATCGTCGCGGCTTCGTG         Fragraria vesca     GTCCAACAAAGACTCCGACCTTTCGGCGGTGCC       Gly80Asp   GGCACCGCCGAAAGGTCGGAGTCTTTGTTGGACCACGAAGCCGC   1318       GGC-GAC   GACGATCTGCGCGACG   T   CGATGTTGCTGCAGTTGCGGCGGGCCT           TGGTGCTGAGCTGGCGGACGAAGTTGGGAGGGA           CAACATCG   A   CGTCGCGC   1319           GCGCGACG   T   CGATGTTG   1320               Met Overproduction   TCTCCTCCCTCATCCTCCGCTTCCCTCCCAACTTCCAGCGCCAGC   1321       CGS   TAAGCACCAAGGCG   A   GCCGCAACTGCAGCAACATCGGCGTCGCG         Glycine max     CAAATCGTCGCCGCTTCGTGGTCGAACAACAG       Arg68His   CTGTTGTTCGACCACGAAGCGGCGACGATTTGCGCGACGCCGAT   1322       CGC-CAC   GTTGCTGCAGTTGCGGC   T   CGCCTTGGTGCTTAGCTGGCGCTGGA           AGTTGGGAGGGAAGCGGAGGATGAGGGAGGAGA           CCAAGGCG   A   GCCGCAAC   1323           GTTGCGGC   T   CGCCTTGG   1324               Met Overproduction   TCCTCCGCTTCCCTCCCAACTTCCAGCGCCAGCTAAGCACCAAGG   1325       CGS   CGCGCCGCAACTGCA   A   CAACATCGGCGTCGCGCAAATCGTCGCC         Glycine max     GCTTCGTGGTCGAACAACAGCGACAACTCTCC       Ser72Asn   GGAGAGTTGTCGCTGTTGTTCGACCACGAAGCGGCGACGATTTG   1326       AGC-AAC   CGCGACGCCGATGTTG   T   TGCAGTTGCGGCGCGCCTTGGTGCTTA           GCTGGCGCTGGAAGTTGGGAGGGAAGCGGAGGA           CAACTGCA   A   CAACATCG   1327           CGATGTTG   T   TGCAGTTG   1328               Met Overproduction   TTCCCTCCCAACTTCCAGCGCCAGCTAAGCACCAAGGCGCGCCG   1329       CGS   CAACTGCAGCAACATC   A   GCGTCGCGCAAATCGTCGCCGCTTCGT         Glycine max     GGTCGAACAACAGCGACAACTCTCCGGCCGCCG       Gly75Ser   CGGCGGCCGGAGAGTTGTCGCTGTTGTTCGACCACGAAGCGGCG   1330       GGC-AGC   ACGATTTGCGCGACGC   T   GATGTTGCTGCAGTTGCGGCGCGCCTT           GGTGCTTAGCTGGCGCTGGAAGTTGGGAGGGAA           GCAACATC   A   GCGTCGCG   1331           CGCGACGC   T   GATGTTGC   1332               Met Overproduction   TCCCTCCCAACTTCCAGCGCCAGCTAAGCACCAAGGCGCGCCGC   1333       CGS   AACTGCAGCAACATCG   A   CGTCGCGCAAATCGTCGCCGCTTCGTG         Glycine max     GTCGAACAACAGCGACAACTCTCCGGCCGCCGG       Gly75Asp   CCGGCGGCGGGAGAGTTGTCGCTGTTGTTCGACCACGAAGCGGC   1334       GGC-GAC   GACGATTTGCGCGACG   T   CGATGTTGCTGCAGTTGCGGCGCGCCT           TGGTGCTTAGCTGGCGCTGGAAGTTGGGAGGGA           CAACATCG   A   CGTCGCGC   1335           GCGCGACG   T   CGATGTTG   1336               Met Overproduction   TGTCTTCTCTGATTTTCAGGTTTCCTCCTAATTTCGTGAGGCAGCT   1337       CGS   AAGCATTAAGGCT   CAC   AGGAATTGCAGCAATATTGGCGTGGCTCA         Solanum tuberosum     AGTTGTGGCGGCTTCCTGGTCTAACAACCA       Arg70His   TGGTTGTTAGACCAGGAAGCCGCCACAACTTGAGCCACGCCAATA   1338       AGG-CAC   TTGCTGCAATTCCT   GTG   AGCCTTAATGCTTAGCTGCCTCACGAAAT           TAGGAGGAAACCTGAAAATCAGAGAAGACA           TAAGGCT   CAC   AGGAATT   1339           AATTCCT   GTG   AGCCTTA   1340               Met Overproduction   TTTTCAGGTTTCCTCCTAATTTCGTGAGGCAGCTAAGCATTAAGGC   1341       CGS   TAGGAGGAATTGCA   A   CAATATTGGCGTGGCTCAAGTTGTGGCGG         Solanum tuberosum     CTTCCTGGTCTAACAACCAAGCCGGTCCTGA       Ser74Asn   TCAGGACCGGCTTGGTTGTTAGACCAGGAAGCCGCCACAACTTG   1342       AGC-AAC   AGCCACGCCAATATTGTTGCAATTCCTCCTAGCCTTAATGCTTAGC           TGCCTCACGAAATTAGGAGGAAACCTGAAAA           GAATTGCA   A   CAATATTG   1343           CAATATTG   T   TGCAATTC   1344               Met Overproduction   TTTCCTCCTAATTTCGTGAGGCAGCTAAGCATTAAGGCTAGGAGG   1345       CGS   AATTGCAGCAATATT   A   GCGTGGCTCAAGTTGTGGCGGCTTCCTGG         Solanum tuberosum     TCTAACAACCAAGCCGGTCCTGAATTCACTC       Gly77Ser   GAGTGAATTCAGGACCGGCTTGGTTGTTAGACCAGGAAGCCGCC   1346       GGC-AGC   ACAACTTGAGCCACGC   T   AATATTGCTGCAATTCCTCCTAGCCTTAA           TGCTTAGCTGCCTCACGAAATTAGGAGGAAA           GCAATATT   A   GCGTGGGT   1347           AGCCACGC   T   AATATTGC   1348               Met Overproduction   TTCCTCCTAATTTCGTGAGGCAGCTAAGCATTAAGGCTAGGAGGA   1349       CGS   ATTGCAGCAATATTG   A   CGTGGCTCAAGTTGTGGCGGCTTCCTGGT         Solanum tuberosum     CTAACAACCAAGCCGGTCCTGAATTCACTCC       Gly77Asp   GGAGTGAATTCAGGACCGGCTTGGTTGTTAGACCAGGAAGCCGC   1350       GGC-GAC   CACAACTTGAGCCACG   T   CAATATTGCTGCAATTCCTCCTAGCCTTA           ATGCTTAGCTGCCTCACGAAATTAGGAGGAA           CAATATTG   A   CGTGGCTC   1351           GAGCCACG   T   CAATATTG   1352               Met Overproduction   CTTCCTCTCTTATCCTTCGCTTTCCTCCCAACTTTGTCCGTCAGCT   1353       CGS   CAGCACCAAGGCTCGCC   A   CAACTGCAGCAACATTGGTGTCGCAC         Mesembryanthemum     AGGTCGTCGCTGCCTCCTGGTCCAACAACTC         crystallinum     GAGTTGTTGGACCAGGAGGCAGCGACGACCTGTGCGACACCAAT   1354       Arg73His   GTTGCTGCAGTTG   T   GGCGAGCCTTGGTGCTGAGCTGACGGACAA       CGC-CAC   AGTTGGGAGGAAAGCGAAGGATAAGAGAGGAAG           GGCTCGCC   A   CAACTGCA   1355           TGCAGTTG   T   GGCGAGCC   1356               Met Overproduction   TCCTTCGCTTTCCTCCCAACTTTGTCCGTCAGCTCAGCACCAAGG   1357       CGS   CTCGCCGCAACTGCAACAACATTGGTGTCGCACAGGTCGTCGCT         Mesembryanthemum     GCCTCCTGGTCCAACAACTCCGATGCCGGCGC         crystallinum     GCGCCGGCATCGGAGTTGTTGGACCAGGAGGCAGCGACGACCT   1358       Ser77Asn   GTGCGACACCAATG   T   TGTTGCAGTTGCGGCGAGCCTTGGTGCTG       AGC-AAC   AGCTGACGGACAAAGTTGGGAGGAAAGCGAAGGA           CAACTGCA   A   CAACATTG   1359           CAATGTTG   T   TGCAGTTG   1360               Met Overproduction   TTTCCTCCCAACTTTGTCCGTCAGCTCAGCACCAAGGCTCGCCGC   1361       CGS   AACTGCAGCAACATT   A   GTGTCGCACAGGTCGTCGCTGCCTCCTG         Mesembryanthemum     GTCCAACAACTCCGATGCCGGCGCCACCTCTT         crystallinum     AAGAGGTGGCGCCGGCATCGGAGTTGTTGGACCAGGAGGCAGC   1362       Gly80Ser   GACGACCTGTGCGACAC   T   AATGTTGCTGCAGTTGCGGCGAGCCT       GGT-AGT   TGGTGCTGAGCTGACGGACAAAGTTGGGAGGAAA           GCAACATT   A   GTGTCGCA   1363           TGCGACAC   T   AATGTTGC   1364               Met Overproduction   TTCCTCCCAACTTTGTCCGTCAGCTCAGCACCAAGGCTCGCCGCA   1365       CGS   ACTGCAGCAACATTG   A   TGTCGCACAGGTCGTCGCTGCCTCCTGGT         Mesembryanthemum     CCAACAACTCCGATGCCGGCGCCACCTCTTG         crystallinum     CAAGAGGTGGCGCCGGCATCGGAGTTGTTGGACCAGGAGGCAG   1366       Gly80Asp   CGACGACCTGTGCGACA   T   CAATGTTGCTGCAGTTGCGGCGAGCC       GGT-GAT   TTGGTGCTGAGCTGACGGACAAAGTTGGGAGGAA           CAACATTG   A   TGTCGCAC   1367           GTGCGACA   T   CAATGTTG   1368               Met Overproduction   CCTCTGCTACCATCCTCCGCTTTCCGCCAAACTTTGTCCGCCAGC   1369       CGS   TTAGCACCAAGGCACACGGCAACTGCAGCAACATCGGCGTCGCG         Zea mays     CAGATCGTCGCCGCCGCGTGGTCCGACTGCCC       Arg41His   GGGCAGTCGGACCACGCGGCGGCGACGATCTGCGCGACGCCGA   1370       CGC-CAC   TGTTGCTGCAGTTGCGG   T   GTGCCTTGGTGCTAAGCTGGCGGACA           AAGTTTGGCGGAAAGCGGAGGATGGTAGCAGAGG           CAAGGCAC   A   CCGCAACT   1371           AGTTGCGG   T   GTGCCTTG   1372               Met Overproduction   TCCTCCGCTTTCCGCCAAACTTTGTCCGCCAGCTTAGCACCAAGG   1373       CGS   CACGCCGCAACTGCA   A   CAACATCGGCGTCGCGCAGATCGTCGCC         Zea mays     GCCGCGTGGTCCGACTGCCCCGCCGCTCGCCC       Ser45Asn   GGGCGAGCGGCGGGGCAGTCGGACCACGCGGCGGCGACGATCT   1374       AGC-AAC   GCGCGACGCCGATGTTG   T   TGCAGTTGCGGCGTGCCTTGGTGCTA           AGCTGGCGGACAAAGTTTGGCGGAAAGCGGAGGA           CAACTGCA   A   CAACATCG   1375           CGATGTTG   T   TGCAGTTG   1376               Met Overproduction   TTTCCGCCAAACTTTGTCCGCCAGCTTAGCACCAAGGCACGCCGC   1377       CGS   AACTGCAGCAACATC   A   GCGTCGCGCAGATCGTCGCCGCCGCGTG         Zea mays     GTCCGACTGCCCCGCCGCTCGCCCCCACTTAG       Gly48Ser   CTAAGTGGGGGCGAGCGGCGGGGCAGTCGGACCACGCGGCGG   1378       GGC-AGC   CGACGATCTGCGCGACGC   T   GATGTTGCTGCAGTTGCGGCGTGCC           TTGGTGCTAAGCTGGCGGACAAAGTTTGGCGGAAA           GCAACATC   A   GCGTCGCG   1379           CGCGACGC   T   GATGTTGC   1380               Met Overproduction   TTCCGCCAAACTTTGTCCGCCAGCTTAGCACCAAGGCACGCCGCA   1381       CGS   ACTGCAGCAACATCG   A   CGTCGCGCAGATCGTCGCCGCCGCGTGG         Zea mays     TCCGACTGCCCCGCCGCTCGCCCCCACTTAGG       Gly48Asp   CCTAAGTGGGGGCGAGCGGCGGGGCAGTCGGACCACGCGGCG   1382       GGC-GAC   GCGACGATCTGCGCGACG   T   CGATGTTGCTGCAGTTGCGGCGTGC           CTTGGTGCTAAGCTGGCGGACAAAGTTTGGCGGAA           CAACATCG   A   CGTCGCGG   1383           GCGCGACG   T   CGATGTTG   1384               Met Overproduction   GTATGAATGATCTGTGGGTGAAACACTGTGGGATTAGTCATACAG   1385       TS   GAAGTTTCAAGGATCGTGGAATGACTGTTTTGGTTAGTCAAGTTAA         Arabidopsis thaliana     TCGTCTGAGAAAGATGAAACGACCTGTGGT       Leu205Arg   ACCACAGGTCGTTTCATCTTTCTCAGACGATTAACTTGACTAACCA   1386       CTT-CGT   AAACAGTCATTCCA   C   GATCCTTGAAACTTCCTGTATGACTAATCCC           ACAGTGTTTCACCCACAGATCATTCATAC           CAAGGATC   G   TGGAATGA   1387           TCATTCCA   C   GATCCTTG   1388               Met Overproduction   GCATGACTGATTTGTGGGTCAAACACTGTGGGATTAGCCATACTG   1389       TS   GTAGTTTTAAGGATCGTGGGATGACTGTTTTGGTGAGTCAAGTTAA         Solanum tuberosum     TCGCTTGCGGAAAATGCATAAACCGGTTGT       Leu198Arg   ACAACCGGTTTATGCATTTTCCGCAAGCGATTAACTTGACTCACCA   1390       CTT-CGT   AAACAGTCATCCCACGATCCTTAAAACTACCAGTATGGCTAATCCC           ACAGTGTTTGACCCACAAATCAGTCATGC           TAAGGATC   G   TGGGATGA   1391           TCATCCCA   C   GATCCTTA   1392               Lys Overproduction   TCATTGGGCACACAGTGAACTGCTTTGGCTCTAGAATCAAAGTGA   1393       DHPS   TAGGCAACACAGGAA   A   CAACTCAACCAGAGAAGCCGTCCACGCA         Zea mays     ACAGAACAGGGATTTGCTGTTGGCATGCATGC       Ser157Asn   GCATGCATGCCAACAGCAAATCCCTGTTCTGTTGCGTGGACGGCT   1394       AGC-AAC   TCTCTGGTTGAGTTG   T   TTCCTGTGTTGCCTATCACTTTGATTCTAG           AGCCAAAGCAGTTCACTGTGTGCCCAATGA           CACAGGAA   A   CAACTCAA   1395           TTGAGTTG   T   TTCCTGTG   1396               Lys Overproduction   GCTCTAGAATCAAAGTGATAGGCAACACAGGAAGCAACTCAACCA   1397       DHPS   GAGAAGCCGTCCACG   A   AACAGAACAGGGATTTGCTGTTGGCATG         Zea mays     CATGCGGCTCTCCACATCAATCCTTACTACGG       Ala166Val   CCGTAGTAAGGATTGATGTGGAGAGCCGCATGCATGCCAACAGC   1398       GCA-GAA   AAATCCCTGTTCTGTT   T   CGTGGACGGCTTCTCTGGTTGAGTTGCTT           CCTGTGTTGCCTATCACTTTGATTCTAGAGC           CGTCCACG   A   AACAGAAC   1399           GTTCTGTT   T   CGTGGACG   1400               Lys Overproduction   GGCTCTAGAATCAAAGTGATAGGCAACACAGGAAGCAACTCAACC   1401       DHPS   AGAGAAGCCGTCCAC   A   CAACAGAACAGGGATTTGCTGTTGGCAT         Zea mays     GCATGCGGCTCTCCACATCAATCCTTACTACG       Ala166Thr   CGTAGTAAGGATTGATGTGGAGAGCCGCATGCATGCCAACAGCA   1402       GCA-ACA   AATCCCTGTTCTGTTG   T   GTGGACGGCTTCTCTGGTTGAGTTGCTTC           CTGTGTTGCCTATCACTTTGATTCTAGAGCC           CCGTCCAC   A   CAACAGAA   1403           TTCTGTTG   T   GTGGACGG   1404               Lys Overproduction   TTATTGGGCATACAGTTAACTGCTTTGGCACTAAAATTAAAGTGGT   1405       DHPS   CGGCAACACAGGAA   A   TAACTCAACAAGGGAGGCTATTCACGCAAC         Oryza sativa     TGAGCAGGGATTCGCTGTAGGTATGCACGC       Ser24Asn   GCGTGCATACCTACAGCGAATCCCTGCTCAGTTGCGTGAATAGCC   1406       AGT-AAT   TCCCTTGTTGAGTTA   T   TTCCTGTGTTGCCGACCACTTTAATTTTAGT           GCCAAAGCAGTTAACTGTATGCCCAATAA           CACAGGAA   A   TAACTCAA   1407           TTGAGTTA   T   TTCCTGTG   1408               Lys Overproduction   GCACTAAAATTAAAGTGGTCGGCAACACAGGAAGTAACTCAACAA   1409       DHPS   GGGAGGCTATTCACGTAACTGAGCAGGGATTCGCTGTAGGTATG         Oryza sativa     CACGCGGCTCTCCACATCAATCCTTACTACGG       Ala133Val   CCGTAGTAAGGATTGATGTGGAGAGCCGCGTGCATACCTACAGC   1410       GCA-GTA   GAATCCCTGCTCAGTT   A   CGTGAATAGCCTCCCTTGTTGAGTTACTT           CCTGTGTTGCCGACCACTTTAATTTTAGTGC           TATTCACG   T   AACTGAGC   1411           GCTCAGTT   A   CGTGAATA   1412               Lys Overproduction   GGCACTAAAATTAAAGTGGTCGGCAACACAGGAAGTAACTCAACA   1413       DHPS   AGGGAGGCTATTCACACAACTGAGCAGGGATTCGCTGTAGGTAT         Oryza sativa     GCACGCGGCTCTCCACATCAATCCTTACTACG       Ala133Thr   CGTAGTAAGGATTGATGTGGAGAGCCGCGTGCATACCTACAGCG   1414       GCA-ACA   AATCCCTGCTCAGTTG   T   GTGAATAGCCTCCCTTGTTGAGTTACTTC           CTGTGTTGCCGACCACTTTAATTTTAGTGCC           CTATTCAC   A   CAACTGAG   1415           CTCAGTTG   T   GTGAATAG   1416               Lys Overproduction   TCATCGGGCATACTGTTAACTGCTTTGGAGCCAACATTAAAGTGAT   1417       DHPS 1   AGGCAACACGGGAA   A   TAACTCAACCAGAGAAGCTGTTCACGCGA         Triticum aestivum     CAGAGCAGGGATTTGCTGTTGGCATGCATGC       Ser65Asn   GCATGCATGCCAACAGCAAATCCCTGCTCTGTCGCGTGAACAGCT   1418       AGT-AAT   TCTCTGGTTGAGTTA   T   TTCCCGTGTTGCCTATCACTTTAATGTTGG           CTCCAAAGCAGTTAACAGTATGCCCGATGA           CACGGGAA   A   TAACTCAA   1419           TTGAGTTA   T   TTCCCGTG   1420               Lys Overproduction   GAGCCAACATTAAAGTGATAGGCAACACGGGAAGTAACTCAACCA   1421       DHPS 1   GAGAAGCTGTTCACGTGACAGAGCAGGGATTTGCTGTTGGCATG         Triticum aestivum     CATGCAGCTCTTCATGTCAATCCTTACTACGG       Ala174Val   CCGTAGTAAGGATTGACATGAAGAGCTGCATGCATGCCAACAGCA   1422       GCG-GTG   AATCCCTGCTCTGTC   A   CGTGAACAGCTTCTCTGGTTGAGTTACTTC           CCGTGTTGCCTATCACTTTAATGTTGGCTC           TGTTCACG   T   GACAGAGC   1423           GCTCTGTC   A   CGTGAACA   1424               Lys Overproduction   GGAGCCAACATTAAAGTGATAGGCAACACGGGAAGTAACTCAACC   1425       DHPS 1   AGAGAAGCTGTTCAC   A   CGACAGAGCAGGGATTTGCTGTTGGCAT         Triticum aestivum     GCATGCAGCTCTTCATGTCAATCCTTACTACG       Ala174Thr   CGTAGTAAGGATTGACATGAAGAGCTGCATGCATGCCAACAGCAA   1426       GCG-ACG   ATCCCTGCTCTGTCG   T   GTGAACAGCTTCTCTGGTTGAGTTACTTCC           CGTGTTGCCTATCACTTTAATGTTGGCTCC           CTGTTCAC   A   GGACAGAG   1427           CTCTGTCG   T   GTGAACAG   1428               Lys Overproduction   TCATCGGGCACACTGTTAACTGCTTTGGAACTAACATTAAAGTGAT   1429       DHPS 2   AGGCAACACGGGAA   A   TAACTCAACTAGAGAAGCGATTCACGCTTC         Triticum aestivum     AGAGCAGGGATTTGCTGTTGGCATGCATGC       Ser154Asn   GCATGCATGCCAACAGCAAATCCCTGCTCTGAAGCGTGAATCGCT   1430       AGT-AAT   TCTCTAGTTGAGTTA   T   TTCCCGTGTTGCCTATCACTTTAATGTTAGT           TCCAAAGCAGTTAACAGTGTGCCCGATGA           CACGGGAA   A   TAACTCAA   1431           TTGAGTTA   T   TTCCCGTG   1432               Lys Overproduction   GAACTAACATTAAAGTGATAGGCAACACGGGAAGTAACTCAACTA   1433       DHPS 2   GAGAAGCGATTCACGTTTCAGAGCAGGGATTTGCTGTTGGCATGC         Triticum aestivum     ATGCAGCTCTCCATGTCAATCCTTACTATGG       Ala163Val   CCATAGTAAGGATTGACATGGAGAGCTGCATGCATGCCAACAGCA   1434       GCT-GTT   AATCCCTGCTCTGAA   A   CGTGAATCGCTTCTCTAGTTGAGTTACTTC           CCGTGTTGCCTATCACTTTAATGTTAGTTC           GATTCACG   T   TTCAGAGC   1435           GCTCTGAA   A   CGTGAATC   1436               Lys Overproduction   GGAACTAACATTAAAGTGATAGGCAACACGGGAAGTAACTCAACT   1437       DHPS 2   AGAGAAGCGATTCACACTTCAGAGCAGGGATTTGCTGTTGGCATG         Triticum aestivum     CATGCAGCTCTCCATGTCAATCCTTACTATG       Ala163Thr   CATAGTAAGGATTGACATGGAGAGCTGCATGCATGCCAACAGCAA   1438       GCT-ACT   ATCCCTGCTCTGAAG   T   GTGAATCGCTTCTCTAGTTGAGTTACTTCC           CGTGTTGCCTATCACTTTAATGTTAGTTCC           CGATTCAC   A   CTTCAGAG   1439           CTCTGAAG   T   GTGAATCG   1440               Lys Overproduction   CTCATTGGGCATACTGTGAACTGCTTTGGCTCTAGAATTAAAGTGA   1441       DHPS   TAGGCAACACAGGAA   A   TAACTCAACCAGAGAAGCTGTTCACGCAA         Coix lacryma - jobi     CAGAGCAGGGATTTGCTGTTGGCATGCATG       Ser154Asn   CATGCATGCCAACAGCAAATCCCTGCTCTGTTGCGTGAACAGCTT   1442       AGT-AAT   CTCTGGTTGAGTTA   T   TTCCTGTGTTGCCTATCACTTTAATTCTAGA           GCCAAAGCAGTTCACAGTATGCCCAATGAG           CACAGGAA   A   TAACTCAA   1443           TTGAGTTA   T   TTCCTGTG   1444               Lys Overproduction   GCTCTAGAATTAAAGTGATAGGCAACACAGGAAGTAACTCAACCA   1445       DHPS   GAGAAGCTGTTCACG   T   AACAGAGCAGGGATTTGCTGTTGGCATGC         Coix lacryma - jobi     ATGCAGCTCTCCACATCAATCCTTACTATGG       Ala163Val   CCATAGTAAGGATTGATGTGGAGAGCTGCATGCATGCCAACAGCA   1446       GCA-GTA   AATCCCTGCTCTGTT   A   CGTGAACAGCTTCTCTGGTTGAGTTACTTC           CTGTGTTGCCTATCACTTTAATTCTAGAGC           TGTTCACG   T   AACAGAGC   1447           GCTCTGTT   A   CGTGAACA   1448               Lys Overproduction   GGCTCTAGAATTAAAGTGATAGGCAACACAGGAAGTAACTCAACC   1449       DHPS   AGAGAAGCTGTTCAC   A   CAACAGAGCAGGGATTTGCTGTTGGCATG         Coix lacryma - jobi     CATGCAGCTCTCCACATCAATCCTTACTATG       Ala163Thr   CATAGTAAGGATTGATGTGGAGAGCTGCATGCATGCCAACAGCAA   1450       GCA-ACA   ATCCCTGCTCTGTTG   T   GTGAACAGCTTCTCTGGTTGAGTTACTTCC           TGTGTTGCCTATCACTTTAATTCTAGAGCC           CTGTTCAC   A   CAACAGAG   1451           CTCTGTTG   T   GTGAACAG   1452               Lys Overproduction   TCATTGGTCACACAGTCAATTGTTTTGGAGGGTCCATCAAAGTCAT   1453       DHPS   CGGGAACACTGGAA   A   CAACTCCACAAGGGAAGCAATCCATGCAA         Nicotiana tabacum     CTGAACAGGGATTTGCTGTAGGTATGCATGC       Ser136Asn   GCATGCATACCTACAGCAAATCCCTGTTCAGTTGCATGGATTGCTT   1454       AGC-AAC   CCCTTGTGGAGTTG   T   TTCCAGTGTTCCCGATGACTTTGATGGACC           CTCCAAAACAATTGACTGTGTGACCAATGA           CACTGGAA   A   CAACTCCA   1455           TGGAGTTG   T   TTCCAGTG   1456               Lys Overproduction   GAGGGTCCATCAAAGTCATCGGGAACACTGGAAGCAACTCCACAA   1457       DHPS   GGGAAGCAATCCATG   T   AACTGAACAGGGATTTGCTGTAGGTATGC         Nicotiana tabacum     ATGCAGCTCTTCACATTAATCCCTACTATGG       Ala145Val   CCATAGTAGGGATTAATGTGAAGAGCTGCATGCATACCTACAGCA   1458       GCA-GTA   AATCCCTGTTCAGTT   A   CATGGATTGCTTCCCTTGTGGAGTTGCTTC           CAGTGTTCCCGATGACTTTGATGGACCCTC           AATCCATG   T   AACTGAAC   1459           GTTCAGTT   A   CATGGATT   1460               Lys Overproduction   GGAGGGTCCATCAAAGTCATCGGGAACACTGGAAGCAACTCCAC   1461       DHPS   AAGGGAAGCAATCCAT   A   CAACTGAACAGGGATTTGCTGTAGGTAT         Nicotiana tabacum     GCATGCAGCTCTTCACATTAATCCCTACTATG       Ala145Thr   CATAGTAGGGATTAATGTGAAGAGCTGCATGCATACCTACAGCAA   1462       GCA-ACA   ATCCCTGTTCAGTTG   T   ATGGATTGCTTCCCTTGTGGAGTTGCTTCC           AGTGTTCCCGATGACTTTGATGGACCCTCC           CAATCCAT   A   CAACTGAA   1463           TTCAGTTG   T   ATGGATTG   1464               Lys Overproduction   TTATAGGCCATACCGTTAACTGTTTTGGCGGAAGCATCAAAGTCAT   1465       DHPS   TGGAAACACTGGAA   A   CAATTCGACTAGAGAAGCAATCCACGCGAC         Arabidopsis thaliana     TGAACAAGGATTCGCGGTTGGAATGCATGC       Ser142Asn   GCATGCATTCCAACCGCGAATCCTTGTTCAGTCGCGTGGATTGCT   1466       AGC-AAC   TCTCTAGTCGAATTG   T   TTCCAGTGTTTCCAATGACTTTGATGCTTC           CGCCAAAACAGTTAACGGTATGGCCTATAA           CACTGGAA   A   CAATTCGA   1467           TCGAATTG   T   TTCCAGTG   1468               Lys Overproduction   GCGGAAGCATCAAAGTCATTGGAAACACTGGAAGCAATTCGACTA   1469       DHPS   GAGAAGCAATCCACG   T   GACTGAACAAGGATTCGCGGTTGGAATG         Arabidopsis thaliana     CATGCTGCTCTTCATATAAACCCTTACTATGG       Ala151Val   CCATAGTAAGGGTTTATATGAAGAGCAGCATGCATTCCAACCGCG   1470       GCG-GTG   AATCCTTGTTCAGTC   A   CGTGGATTGCTTCTCTAGTCGAATTGCTTC           CAGTGTTTCCAATGACTTTGATGCTTCCGC           AATCCACG   T   GACTGAAC   1471           GTTCAGTC   A   CGTGGATT   1472               Lys Overproduction   GGCGGAAGCATCAAAGTCATTGGAAACACTGGAAGCAATTCGACT   1473       DHPS   AGAGAAGCAATCCAC   A   CGACTGAACAAGGATTCGCGGTTGGAAT         Arabidopsis thaliana     GCATGCTGCTCTTCATATAAACCCTTACTATG       Ala151Thr   CATAGTAAGGGTTTATATGAAGAGCAGCATGCATTCCAACCGCGA   1474       GCG-ACG   ATCCTTGTTCAGTCG   T   GTGGATTGCTTCTCTAGTCGAATTGCTTCC           AGTGTTTCCAATGACTTTGATGCTTCCGCC           CAATCCAC   A   CGACTGAA   1475           TTCAGTCG   T   GTGGATTG   1476               Lys Overproduction   TTATTGCTCATACAGTCAACTGTTTTGGTGGGAAAATTAAGGTTAT   1477       DHPS   TGGAAATACTGGAA   A   CAACTCCACCAGGGAAGCAATTCATGCCAC         Glycine max     TGAGCAGGGTTTTGCTGTTGGAATGCATGC       Ser103Asn   GCATGCATTCCAACAGCAAAACCCTGCTCAGTGGCATGAATTGCT   1478       AGC-AAC   TCCCTGGTGGAGTTGTTTCCAGTATTTCCAATAACCTTAATTTTCC           CACCAAAACAGTTGACTGTATGAGCAATAA           TACTGGAA   A   CAACTCCA   1479           TGGAGTTG   T   TTCCAGTA   1480               Lys Overproduction   GTGGGAAAATTAAGGTTATTGGAAATACTGGAAGCAACTCCACCA   1481       DHPS   GGGAAGCAATTCATG   T   CACTGAGCAGGGTTTTGCTGTTGGAATGC         Glycine max     ATGCTGCCCTTCACATAAACCCTTACTATGG       Ala112Val   CCATAGTAAGGGTTTATGTGAAGGGCAGCATGCATTCCAACAGCA   1482       GCC-GTC   AAACCCTGCTCAGTG   A   CATGAATTGCTTCCCTGGTGGAGTTGCTT           CCAGTATTTCCAATAACCTTAATTTTCCCAC           AATTCATG   T   CACTGAGC   1483           GCTCAGTG   A   CATGAATT   1484               Lys Overproduction   GGTGGGAAAATTAAGGTTATTGGAAATACTGGAAGCAACTCCACC   1485       DHPS   AGGGAAGCAATTCAT   A   CCACTGAGCAGGGTTTTGCTGTTGGAATG         Glycine max     CATGCTGCCCTTCACATAAACCCTTACTATG       Ala112Thr   CATAGTAAGGGTTTATGTGAAGGGCAGCATGGATTCCAACAGCAA   1486       GCC-ACC   AACCCTGCTCAGTGG   T   ATGAATTGCTTCCCTGGTGGAGTTGCTTC           CAGTATTTCCAATAACCTTAATTTTCCCACC           CAATTCAT   A   CCACTGAG   1487           CTCAGTGG   T   ATGAATTG   1488               Trp Overproduction   CTTGCAGGAGACATATTTCAGATCGTGCTGAGTCAACGTTTTGAG   1489       AS   CGGCGAACATTTGCA   A   ACCCCTTTGAAGTTTATAGAGCACTAAGA         Arabidopsis thaliana     GTTGTGAATCCAAGTCCGTATATGGGTTATT       Asp341Asn   AATAACCCATATACGGACTTGGATTCACAACTCTTAGTGCTCTATA   1490       GAG-AAC   AACTTCAAAGGGGT   T   TGCAAATGTTCGCCGCTCAAAACGTTGACT           CAGCACGATCTGAAATATGTCTCCTGCAAG           CATTTGCA   A   ACCCCTTT   1491           AAAGGGGT   T   TGCAAATG   1492               Trp Overproduction   GCTGCAGGAGACATATTTCAAATCGTTTTAAGTCAACGCTTTGAGA   1493       AS   GAAGAACATTTGCT   A   ACCCATTTGAAGTGTACAGAGCATTAAGAAT         Nicotiana tabacum     TGTGAATCCAAGCCCATATATGACTTACA       Asp326Asn   TGTAAGTCATATATGGGCTTGGATTCACAATTCTTAATGCTCTGTA   1494       GAC-AAC   CACTTCAAATGGGT   T   AGCAAATGTTCTTCTCTCAAAGCGTTGACTT           AAAACGATTTGAAATATGTCTCCTGCAGC           CATTTGCT   A   ACCCATTT   1495           AAATGGGT   T   AGCAAATG   1496               Trp Overproduction   CTAGCTGGTGACATTTTTCAAGTAGTCTTAAGCCAGCGTTTTGAGA   1497       AS   GGCGTACATTTGCT   A   ACCCCTTTGAGGTGTACCGTGCATTGCGTA         Oryza sativa     TTGTCAATCCTAGTCCTTATATGGCCTATC       Asp323Asn   GATAGGCCATATAAGGACTAGGATTGACAATACGCAATGCACGGT   1498       GAC-AAC   ACACCTCAAAGGGGT   T   AGCAAATGTACGCCTCTCAAAACGCTGGC           TTAAGACTACTTGAAAAATGTCACCAGCTAG           CATTTGCT   A   ACCCCTTT   1499           AAAGGGGT   T   AGCAAATG   1500               Trp Overproduction   CTTGCTGGTGACATATTCCAGATCGTACTAAGTCAGCGTTTTGAAA   1501       AS   GGCGAACGTTCGCA   A   ACCCATTTGAAATCTATAGATCACTGAGGA         Ruta graveolens     TTGTTAATCCAAGCCCATATATGACTTATT       Asp354Asn   AATAAGTCATATATGGGCTTGGATTAACAATCCTCAGTGATCTATA   1502       GAC-AAC   GATTTCAAATGGGT   T   TGCGAACGTTCGCCTTTCAAAACGCTGACTT           AGTACGATCTGGAATATGTCACCAGCAAG           CGTTCGCA   A   ACCCATTT   1503           AAATGGGT   T   TGCGAACG   1504               Trp Overproduction   CTGGCTGGGGACATATTCCAGCTTGTCCTAAGTCAGCGTTTTGAA   1505       AS   CGGCGAACATTTGCA   A   ATCCATTTGAAGTCTACCGAGCATTGAGA         Catharanthus roseus     ATTGTCAACCCAAGTCCATATATGACTTATT       Asp354Asn   AATAAGTCATATATGGACTTGGGTTGACAATTCTCAATGCTCGGTA   1506       GAT-AAT   GACTTCAAATGGAT   T   TGCAAATGTTCGCCGTTCAAAACGCTGACTT           AGGACAAGCTGGAATATGTCCCCAGCCAG           CATTTGCA   A   ATCCATTT   1507           AAATGGAT   T   TGCAAATG   1508                  
 
     EXAMPLE 10  
     Production of Modified Starch in Plants  
     [0138] A principal aim of biotechnology is the improvement of crop plants for food value, agriculture, and to produce a range of plant-derived raw materials. Along with oils, fats and proteins, polysaccharides constitute the main raw materials derived from plants, and apart from cellulose, the storage polymer starch is the most important polysaccharide raw material. Starch is derived from a range of plants, but maize is the most important cultivated plant for the production of starch.  
     [0139] The polysaccharide starch is a polymer made up of glucose molecules. However, starch is not a homogeneous raw material and is, in fact, a highly complex mixture of various types of molecules which differ from each other, for example, in their degree of polymerization and in the degree of branching of the glucose chains. For example, amylose-starch is a basically non-branched polymer made up of α-1,4-glycosidically branched glucose molecules, and amylopectin-starch is a complex mixture of variously branched glucose chains. The branching results from additional α-1,6-glycosidic linkages. In plants from which starch is typically isolated, for example maize or potato, the starch is approximately 25% amylose-starch and 75% amylopectin-starch.  
     [0140] In maize, various mutants in starch metabolism are known, for example waxy, sugary, shrunken and opaque-2. In addition to producing a modified starch, these mutations greatly improve grain quality in maize, and thus expand the use of maize not only as the food but also for the important industrial materials in food chemistry. It would therefore be advantageous to be able readily to obtain mutants in these genes in particular maize genotypes as well as other plants. Such plants can be obtained, for example, using traditional breeding methods and through specific genetic modification by means of recombinant DNA techniques.  
     [0141] The attached tables disclose exemplary oligonucleotide base sequences which can be used to generate site-specific mutations in genes involved in starch metabolism.  
                   TABLE 20                          Genome-Altering Oligos Conferring Increased Starch                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               Increased Starch   GAACTTGAGACTGAGAAAAGGGATCCAAGGACAGTTGCTTCCATT   1509           ADPGPP   ATTCTTGGAGGTGGA   AA   AGGAACTCGACTCTTTCCTCTCACAAAA         Arabidopsis thaliana     CGCCGCGCCAAGCCTGCCGTTCCTATCGGGG       Ala99Lys   CCCCGATAGGAACGGCAGGCTTGGCGCGGCGTTTTGTGAGAGGA   1510       GCA-AAA   AAGAGTCGAGTTCCT   TT   TCCACCTCCAAGAATAATGGAAGCAACT           GTCCTTGGATCCCTTTTCTCAGTCTCAAGTTC           GAGGTGGA   AA   AGGAACT   1511           AGTTCCT   TT   TCCACCTC   1512               Increased Starch   CAAAACGCCGCGCCAAGCCTGCCGTTCCTATCGGGGGAGCCTAT   1513       ADPGPP   AGGTTGATAGATGTAC   T   AATGAGCAATTGTATTAACAGCGGAATCA         Arabidopsis thaliana     ACAAAGTCTACATACTCACACAATATAACTC       Pro127Leu   GAGTTATATTGTGTGAGTATGTAGACTTTGTTGATTCCGCTGTTAA   1514       CCA-CTA   TACAATTGCTCATT   A   GTACATCTATCAACCTATAGGCTCCCCCGAT           AGGAACGGCAGGCTTGGCGCGGCGTTTTG           AGATGTAC   T   AATGAGCA   1515           TGCTCATT   A   GTACATCT   1516               Increased Starch   TCACACAATATAACTCAGCATCATTGAACAGGCATTTAGCCCGTGC   1517       ADPGPP   TTACAACTCCAAT   AAT   CTTGGCTTTGGAGATGGCTATGTTGAGGTT         Arabidopsis thaliana     CTTGCGGCCACTCAAACGCCAGGAGAATC       Gly162Asn   GATTCTCCTGGCGTTTGAGTGGCCGCAAGAACCTCAACATAGCCA   1518       GGA-AAT   TCTCCAAAGCCAAG   ATT   ATTGGAGTTGTAAGCACGGGGTAAATGC           CTGTTCAATGATGCTGAGTTATATTGTGTGA           CTCCAAT   AAT   CTTGGCT   1519           AGCCAAG   ATT   ATTGGAG   1520               Increased Starch   TCACACAATATAACTCAGCATCATTGAACAGGCATTTAGCCCGTGC   1521       ADPGPP   TTACAACTCCAAT   AAC   CTTGGCTTTGGAGATGGCTATGTTGAGGTT         Arabidopsis thaliana     CTTGCGGCCACTCAAACGCCAGGAGAATC       Gly162Asn   GATTCTCCTGGCGTTTGAGTGGCCGCAAGAACCTCAACATAGCCA   1522       GGA-AAC   TCTCCAAAGCCAAG   GTT   ATTGGAGTTGTAAGCACGGGCTAAATGC           CTGTTCAATGATGCTGAGTTATATTGTGTGA           CTCCAAT   AAC   CTTGGCT   1523           AGCCAAG   GTT   ATTGGAG   1524               Increased Starch   GTTTGAGAGAAGAAAGGTAGACCCGCAAAATGTGGCTGCAATCAT   1525       ADPGPP   TCTAGGAGGAGGCAA   A   GGAGCTAAACTCTTCCCTCTTACAATGAG         Arabidopsis thaliana     AGCCGCAACACCAGCTGTAAATATTCATCTT       Asn100Lys   AAGATGAATATTTACAGCTGGTGTTGCGGCTCTCATTGTAAGAGG   1526       AAT-AAA   GAAGAGTTTAGCTCC   T   TTGCCTCCTCCTAGAATGATTGCAGCCAC           ATTTTGCGGGTCTACCTTTCTTCTCTCAAAC           GGAGGCAA   A   GGAGCTAA   1527           TTAGCTCC   T   TTGCCTCC   1528               Increased Starch   CTTGTGTCTTCAAATTATGTTAGGTTCCTGTTGGTGGATGCTACAG   1529       ADPGPP   GCTGATCGATATCC   T   GATGAGTAACTGTATTAACAGCTGCATCAAC         Arabidopsis thaliana     AAGATATTTGTGCTGACACAGTTCAACTC       Pro128Leu   GAGTTGAACTGTGTCAGCACAAATATCTTGTTGATGCAGCTGTTAA   1530       CCG-CTG   TACAGTTACTCATC   A   GGATATCGATCAGCCTGTAGCATCCACCAA           CAGGAACCTAACATAATTTGAAGACACAAG           CGATATCC   T   GATGAGTA   1531           TACTCATC   A   GGATATCG   1532               Increased Starch   TGACACAGTTCAACTCAGCTTCCCTTAATCGACATTTAGCACGAAC   1533       ADPGPP   TTATTTTGGGAAT   AAT   ATAAACTTTGGAGGTGGTTTCGTAGAGGTA         Arabidopsis thaliana     CAAACACTATGACAATAATAACTCTCAGC       Gly163Asn   GCTGAGAGTTATTATTGTCATAGTGTTTGTACCTCTACGAAACCAC   1534       GGC-AAT   CTCCAAAGTTTAT   ATT   ATTCCCAAAATAAGTTCGTGCTAAATGTCG           ATTAAGGGAAGCTGAGTTGAACTGTGTCA           TGGGAAT   AAT   ATAAACT   1535           AGTTTAT   ATT   ATTCCCA   1536               Increased Starch   TGACACAGTTCAACTCAGCTTCCCTTAATCGACATTTAGCACGAAC   1537       ADPGPP   TTATTTTGGGAAT   AAC   ATAAACTTTGGAGGTGGTTTCGTAGAGGTA         Arabidopsis thaliana     CAAACACTATGACAATAATAACTCTCAGC       Gly163Asn   GCTGAGAGTTATTATTGTCATAGTGTTTGTACCTCTACGAAACCAC   1538       GGC-AAC   CTCCAAAGTTTAT   GTT   ATTCCCAAAATAAGTTCGTGCTAAATGTCG           ATTAAGGGAAGCTGAGTTGAACTGTGTCA           TGGGAAT   AAC   ATAAACT   1539           AGTTTAT   GTT   ATTCCCA   1540               Increased Starch   TTGAGGAACAACCAACGGCAGATCCAAAAGCTGTTGCCTCTGTCA   1541       ADPGPP   TTCTAGGTGGTGGT   AAA   GGAACTCGTCTTTTTCCTCTTACAAGCA         Lycopersicon     GAAGAGCTAAACCAGCTGTTCCTATTGGTGG         esculentum     CCACCAATAGGAACAGCTGGTTTAGCTCTTCTGCTTGTAAGAGGA   1542       Val94Lys   AAAAGACGAGTTCC   TTT   ACCACCACCTAGAATGACAGAGGCAACA       GTT-AAA   GCTTTTGGATCTGCCGTTGGTTGTTCCTCAA           TGGTGGT   AAA   GGAACTC   1543           GAGTTCC   TTT   ACCACCA   1544               Increased Starch   CAAGCAGAAGAGCTAAACCAGCTGTTCCTATTGGTGGTTGTTACC   1545       ADPGPP   GGCTAATTGATGTAC   A   AATGAGTAACTGCATTAACAGTGGCATAC         Lycopersicon     GGAAAATTTTCATCTTAACACAGTTCAATTC         esculentum     GAATTGAACTGTGTTAAGATGAAAATTTTCCGTATGCCACTGTTAA   1546       Pro122Leu   TGCAGTTACTCATT   T   GTACATCAATTAGCCGGTAACAACCACCAAT       CCA-CAA   AGGAACAGCTGGTTTAGCTCTTCTGGTTG           TGATGTAC   A   AATGAGTA   1547           TACTCATT   T   GTACATCA   1548               Increased Starch   CACAGTTCAATTCCTTTTCCCTCAATCGTCACCTTGCCCGCACGTA   1549       ADPGPP   TAATTTTGGAAAT   AAT   GTGGGTTTTGGAGATGGATTTGTGGAGGTT         Lycopersicon     TTAGCTGCAACCCAGACTCCAGGGGATGC         esculentum     GCATCCCCTGGAGTCTGGGTTGCAGCTAAAACCTCCACAAATCCA   1550       Gly158Asn   TCTCCAAAACCCAC   ATT   ATTTCCAAAATTATACGTGCGGGCAAGGT       GGA-AAT   GACGATTGAGGGAAAAGGAATTGAACTGTG           TGGAAAT   AAT   GTGGGTT   1551           AACCCAC   ATT   ATTTCCA   1552               Increased Starch   CACAGTTCAATTCCTTTTCCCTCAATCGTCACCTTGCCCGCACGTA   1553       ADPGPP   TAATTTTGGAAAT   AAC   GTGGGTTTTGGAGATGGATTTGTGGAGGT         Lycopersicon     TTTAGCTGCAACCCAGACTCCAGGGGATGC         esculentum     GCATCCCCTGGAGTCTGGGTTGCAGCTAAAACCTCCACAAATCCA   1554       Gly158Asn   TCTCCAAAACCCAC   GTT   ATTTCCAAAATTATACGTGCGGGCAAGGT       GGA-AAC   GACGATTGAGGGAAAAGGAATTGAACTGTG           TGGAAAT   AAC   GTGGGTT   1555           AACCCAC   GTT   ATTTCCA   1556               Increased Starch   ACGTAGATTTGGAAAAAAGAGACCCAAGTACAGTTGTAGCAATTAT   1557       ADPGPP   ACTAGGTGGAGGT   AAA   GGAACTCGTCTCTTCCCTCTCACCAAGCG         Cicer arietinum     ACGAGCCAAGCCTGCTGTTCCAATTGGAGG       Ala101Lys   CCTCCAATTGGAACAGCAGGCTTGGCTCGTCGCTTGGTGAGAGG   1558       GCT-AAA   GAAGAGACGAGTTCC   TTT   ACCTCCACCTAGTATAATTGCTACAACT           GTACTTGGGTCTCTTTTTTCCAAATCTACGT           TGGAGGT   AAA   GGAACTC   1559           GAGTTCC   TTT   ACCTCCA   1560               Increased Starch   CCAAGCGACGAGCCAAGCCTGCTGTTCCAATTGGAGGTGCTTATA   1561       ADPGPP   GGCTGATAGATGTAC   T   AATGAGTAACTGCATCAATAGTGGGATCA         Cicer arietinum     ACAAAGTATACATTCTCACTCAATTTAATTC       Pro129Leu   GAATTAAATTGAGTGAGAATGTATACTTTGTTGATCCCACTATTGA 1562       CCA-CTA   TGCAGTTACTCATT   A   GTACATCTATCAGCCTATAAGCACCTCCAAT           TGGAACAGCAGGCTTGGCTCGTCGCTTGG           AGATGTAC   T   AATGAGTA   1563           TACTCATT   A   GTACATCT   1564               Increased Starch   CTCAATTTAATTCAGCCTCACTCAACAGGCATATTGCACGTGCTTA   1565       ADPGPP   TAACTCTGGTACT   AAT   GTCACTTTTGGAGATGGCTATGTTGAGGTT         Cicer arietinum     CTTGCAGCAACTCAAACTCCAGGGGAGCA       Gly165Asn   TGCTCCCGTGGAGTTTGAGTTGCTGCAAGAACCTCAACATAGCCA   1566       GGA-AAT   TCTCCAAAAGTGAC   ATT   AGTACCAGAGTTATAAGCACGTGCAATAT           GCCTGTTGAGTGAGGCTGAATTAAATTGAG           TGGTACT   AAT   GTCACTT   1567           AAGTGAC   ATT   AGTACCA   1568               Increased Starch   CTCAATTTAATTCAGCCTCACTCAACAGGCATATTGCACGTGCTTA   1569       ADPGPP   TAACTCTGGTACT   AAC   GTCACTTTTGGAGATGGCTATGTTGAGGTT         Cicer arietinum     CTTGCAGCAACTCAAACTCCAGGGGAGCA       Gly165Asn   TGCTCCCCTGGAGTTTGAGTTGCTGCAAGAACCTCAACATAGCCA   1570       GGA-AAC   TCTCCAAAAGTGAC   GTT   AGTACCAGAGTTATAAGCACGTGCAATAT           GCCTGTTGAGTGAGGCTGAATTAAATTGAG           TGGTACT   AAC   GTCACTT   1571           AAGTGAC   GTT   AGTACCA   1572               Increased Starch   ATATTGGAGAGGCGTCGGGCAAACCCTAAGAATGTGGCTGCAATC 1573       ADPGPP   ATACTGCCAGGCGGT   AA   AGGGACACACCTATTCCCTCTCACCAAT         Ipomoea batatas     CGAGCTGCAACCCCTGCTGTTCCACTTGGAG       Ala94Lys   CTCCAAGTGGAACAGCAGGGGTTGCAGCTCGATTGGTGAGAGGG   1574       GCA-AAA   AATAGGTGTGTCCCT   TT   ACCGCCTGGCAGTATGATTGCAGCCACA           TTCTTAGGGTTTGCCCGACGCCTCTCCAATAT           CAGGCGGT   AA   AGGGACA   1575           TGTCCCT   TT   ACCGCCTG   1576               Increased Starch   CCAATCGAGCTGCAACCCCTGCTGTTCCACTTGGAGGATGCTATA   1577       ADPGPP   GGTTGATCGACATTC   T   AATGAGCAACTGCATCAACAGCGGGGTTA         Ipomoea batatas     ACAAGATCTTTGTGCTGACCCAGTTCAATTC       Pro122Leu   GAATTGAACTGGGTCAGCACAAAGATCTTGTTAACCCCGCTGTTG   1578       CCA-CTA   ATGCAGTTGCTCATT   A   GAATGTCGATCAACCTATAGCATCCTCCAA           GTGGAACAGCAGGGGTTGCAGCTCGATTGG           CGACATTC   T   AATGAGCA   1579           TGCTCATT   A   GAATGTCG   1580               Increased Starch   TGACCCAGTTCAATTCAGCTTCTCTTAACCGTCACATTTCCCGTAC   1581       ADPGPP   CGTCTTTGGCAAT   AAT   GTGAGCTTCGGAGATGGATTTGTTGAGGT         Ipomoea batatas     GCTGGCTGCAACCCAAACACAAGGGGAAAC       Gly157Asn   GTTTCCCCTTGTGTTTGGGTTGCAGCCAGCACCTCAACAAATCCA   1582       GGT-AAT   TCTCCGAAGCTCAC   ATT   ATTGCCAAAGACGGTACGGGAAATGTGA           CGGTTAAGAGAAGCTGAATTGAACTGGGTCA           TGGCAAT   AAT   GTGAGCT   1583           AGCTCAC   ATT   ATTGCCA   1584               Increased Starch   TGACCCAGTTCAATTCAGCTTCTCTTAACCGTCACATTTCCCGTAC   1585       ADPGPP   CGTCTTTGGCAAT   AAC   GTGAGCTTCGGAGATGGATTTGTTGAGGT         Ipomoea batatas     GCTGGCTGCAACCCAAACACAAGGGGAAAC       Gly157Asn   GTTTCCCCTTGTGTTTGGGTTGCAGCCAGCACCTCAACAAATCCA   1586       GGT-AAC   TCTCCGAAGCTCAC   GTT   ATTGCCAAAGACGGTACGGGAAATGTGA           CGGTTAAGAGAAGCTGAATTGAACTGGGTCA           TGGCAAT   AAC   GTGAGCT   1587           AGCTCAC   GTT   ATTGCCA   1588               Increased Starch   CATTCCGGAGGAACTTTGCGGATCCAAATGAGGTTGCTGCTGTTA   1589       ADPGPP   TATTGGGTGGTGGCA   AA   GGGACTCAACTTTTTCCTCTCACAAGCA         Oryza sativa     CAAGGGCCACGCCTGCTGTTCCTATTGGAGG       Thr96Lys   CCTCCAATAGGAACAGCAGGCGTGGCCCTTGTGCTTGTGAGAGG   1590       ACC-AAA   AAAAAGTTGAGTCCC   TT   TGCCACCACCCAATATAACAGCAGCAAC           CTCATTTGGATCCGCAAAGTTCCTCCGGAATG           TGGTGGCA   AA   GGGACTC   1591           GAGTCCC   TT   TGCCACCA   1592               Increased Starch   CAAGCACAAGGGCCACGCCTGCTGTTCCTATTGGAGGATGCTATA   1593       ADPGPP   GGCTTATCGATATCC   T   CATGAGCAACTGTTTCAACAGTGGCATAAA         Oryza sativa     CAAGATATTCATAATGACTCAATTCAACTC       Pro124Leu   GAGTTGAATTGAGTCATTATGAATATCTTGTTTATGCCACTGTTGA   1594       CCC-CTC   AACAGTTGCTCATG   A   GGATATCGATAAGCCTATAGCATCCTCCAAT           AGGAACAGCAGGCGTGGCCCTTGTGCTTG           CGATATCC   T   CATGAGCA   1595           TGCTCATG   A   GGATATCG   1596               Increased Starch   TGACTCAATTCAACTCAGCATCTCTTAATCGTCACATTCATCGTAC   1597       ADPGPP   GTACCTTGGTGGT   AAT   ATCAACTTTACTGATGGTTCTGTTGAGGTA         Oryza sativa     TTAGCCGCTACACAAATGCCTGGGGAGGC       Gly159Asn   GCCTCCCCAGGCATTTGTGTAGCGGCTAATACCTCAACAGAACCA   1598       GGA-AAT   TCAGTAAAGTTGAT   ATT   ACCACCAAGGTACGTACGATGAATGTGA           CGATTAAGAGATGCTGAGTTGAATTGAGTCA           TGGTGGT   AAT   ATCAACT   1599           AGTTGAT   ATT   ACCACCA   1600               Increased Starch   TGACTCAATTCAACTCAGCATCTCTTAATCGTCACATTCATCGTAC   1601       ADPGPP   GTACCTTGGTGGT   AAC   ATCAACTTTACTGATGGTTCTGTTGAGGTA         Oryza sativa     TTAGCCGCTACACAAATGCCTGGGGAGGC       Gly159Asn   GCCTCCCCAGGCATTTGTGTAGCGGCTAATACCTCAACAGAACCA   1602       GGA-AAC   TCAGTAAAGTTGAT   GTT   ACCACCAAGGTACGTACGATGAATGTGA           CGATTAAGAGATGCTGAGTTGAATTGAGTCA           TGGTGGT   AAC   ATCAACT   1603           AGTTGAT   GTT   ACCACCA   1604               Increased Starch   GTCCTTCAGGAGGATTAAGCGATCCGAACGAGGTTGCGGCCGTC   1605       ADPGPP   ATACTCGGCGGCGGCA   AA   GGGACTCAGCTCTTCCCACTCACGAG         Triticum aestivum     CACAAGGGCCACACCTGCTGTTCCTATTGGAGG       Thr80Lys   CCTCCAATAGGAACAGCAGGTGTGGCCCTTGTGCTCGTGAGTGG   1606       ACC-AAA   GAAGAGCTGAGTCCC   TT   TGCCGCCGCCGAGTATGACGGCCGCAA           CCTCGTTCGGATCGCTTAATCCTCCTGAAGGAC           CGGCGGCA   AA   GGGACTC   1607           GAGTCCC   TT   TGCCGCCG   1608               Increased Starch   CGAGCACAAGGGCCACACCTGCTGTTCCTATTGGAGGATGTTACA   1609       ADPGPP   GGCTCATCGACATTC   T   CATGAGCAACTGCTTCAACAGTGGCATCA         Triticum aestivum     ACAAGATATTCGTCATGACCCAGTTCAACTC       Pro108Leu   GAGTTGAACTGGGTCATGACGAATATCTTGTTGATGCCACTGTTG   1610       CCC-CTC   AAGCAGTTGCTCATG   A   GAATGTCGATGAGCCTGTAACATCCTCCA           ATAGGAACAGCAGGTGTGGCCCTTGTGCTCG           CGACATTC   T   CATGAGCA   1611           TGCTCATG   A   GAATGTCG   1612               Increased Starch   TGACCCAGTTCAACTCGGCCTCCCTTAATCGTCACATTCACCGCA   1613       ADPGPP   CCTACCTCGGCGGG   AAT   ATCAATTTCACTGATGGATCCGTTGAGG         Triticum aestivum     TATTGGCCGCGACGCAAATGCCCGGGGAGGC       Gly143Asn   GCCTCCCCGGGCATTTGCGTCGCGGCCAATACCTCAACGGATCC   1614       GGA-AAT   ATCAGTGAAATTGAT   ATT   CCCGCCGAGGTAGGTGCGGTGAATGTG           ACGATTAAGGGAGGCCGAGTTGAACTGGGTCA           CGGCGGG   AAT   ATCAATT   1615           AATTGAT   ATT   CCCGCCG   1616               Increased Starch   TGACCCAGTTCAACTCGGCCTCCCTTAATCGTCACATTCACCGCA   1617       ADPGPP   CCTACCTCGGCGGG   AAC   ATCAATTTCACTGATGGATCCGTTGAGG         Triticum aestivum     TATTGGCCGCGACGCAAATGCCCGGGGAGGC       Gly143Asn   GCCTCCCCGGGCATTTGCGTCGCGGCCAATACCTCAACGGATCC   1618       GGA-AAC   ATCAGTGAAATTGAT   GTT   CCCGCCGAGGTAGGTGCGGTGAATGTG           ACGATTAAGGGAGGCCGAGTTGAACTGGGTCA           CGGCGGG   AAC   ATCAATT   1619           AATTGAT   GTT   CCCGCCG   1620               Increased Starch   CCTCCCGAAAGAATTATGCTGATGCAAGCCACGTTTCTGCTGTCA   1621       ADPGPP   TTTTGGGTGGAGGCA   AA   GGAGTTCAACTCTTTCCTCTGACAAGCA         Oryza sativa     CAAGGGCTACCCCCGCTGTTCCTGTTGGAGG       Thr95Lys   CCTCCAACAGGAACAGCGGGGGTAGCCCTTGTGCTTGTCAGAGG   1622       ACT-AAA   AAAGAGTTGAACTCC   TT   TGCCTCCACCCAAAATGACAGCAGAAAC           GTGGCTTGCATCAGCATAATTCTTTCGGGAGG           TGGAGGCA   AA   GGAGTTC   1623           GAACTCC   TT   TGCCTCCA   1624               Increased Starch   CAAGCACAAGGGCTACCCCCGCTGTTCCTGTTGGAGGATGTTACA   1625       ADPGPP   GGCTTATTGACATCC   T   TATGAGCAATTGCTTCAATAGCGGAATAAA         Oryza sativa     TAAAATATTTGTGATGACTCAGTTCAATTC       Pro123Leu   GAATTGAACTGAGTCATCACAAATATTTTATTTATTCCGCTATTGAA   1626       CCT-CTT   GCAATTGCTCATA   A   GGATGTCAATAAGCCTGTAACATCCTCCAACA           GGAACAGCGGGGGTAGCCCTTGTGCTTG           TGACATCC   T   TATGAGCA   1627           TGCTCATA   A   GGATGTCA   1628               Increased Starch   TGACTCAGTTCAATTCTGCTTCTCTTAATCGCCATATCCATCATACA   1629       ADPGPP   TACCTTGGTGGG   AAT   ATCAACTTTACTGATGGGTCTGTGCAGGTA         Oryza sativa     TTGGCTGCTACACAAATGCCTGACGAACC       Gly158Asn   GGTTCGTCAGGCATTTGTGTAGCAGCCAATACCTGCACAGACCCA   1630       GGG-AAT   TCAGTAAAGTTGAT   ATT   CCCACCAAGGTATGTATGATGGATATGGC           GATTAAGAGAAGCAGAATTGAACTGAGTCA           TGGTGGG   AAT   ATCAACT   1631           AGTTGATATTCCCACCA   1632               Increased Starch   TGACTCAGTTCAATTCTGCTTCTCTTAATCGCCATATCCATCATACA   1633       ADPGPP   TACCTTGGTGGG   AAC   ATCAACTTTACTGATGGGTCTGTGCAGGTA         Oryza sativa     TTGGCTGCTACACAAATGCCTGACGAACC       Gly158Asn   GGTTCGTCAGGCATTTGTGTAGCAGCCAATACCTGCACAGACCCA   1634       GGG-AAC   TCAGTAAAGTTGAT   GTT   CCCACCAAGGTATGTATGATGGATATGG           CGATTAAGAGAAGCAGAATTGAACTGAGTCA           TGGTGGG   AAC   ATCAACT   1635           AGTTGAT   GTT   CCCACCA   1636               Increased Starch   CCTTCCGCAGGAATTACGCCGATCCGAACGAGGTCGCGGCCGTC   1637       ADPGPP   ATACTCGGCGGTGGCAAAGGGACTCAGCTCTTCCCTCTCACAAG         Triticum pestivum     CACAAGGGCCACACCTGCTGTTCCTATTGGAGG       Thr99Lys   CCTCCAATAGGAACAGCAGGTGTGGCCCTTGTGCTTGTGAGAGG   1638       ACC-AAA   GAAGAGCTGAGTCCC   TT   TGCCACCGCCGAGTATGACGGCCGCGA           CCTCGTTCGGATCGGCGTAATTCCTGCGGAAGG           CGGTGGCA   AA   GGGACTC   1639           GAGTCCC   TT   TGCCACCG   1640               Increased Starch   CAAGCACAAGGGCCACACCTGCTGTTCCTATTGGAGGATGTTACA   1641       ADPGPP   GGCTCATCGATATTC   T   CATGAGCAACTGCTTCAATAGTGGCATCAA         Triticum aestivum     CAAGATATTCGTCATGACGCAGTTCAACTC       Pro127Leu   GAGTTGAACTGCGTCATGACGAATATCTTGTTGATGCCACTATTGA   1642       CCC-CTC   AGCAGTTGCTCATG   A   GAATATCGATGAGCCTGTAACATCCTCCAA           TAGGAACAGCAGGTGTGGCCCTTGTGCTTG           CGATATTC   T   CATGAGCA   1643           TGCTCATG   A   GAATATCG   1644               Increased Starch   TGACGCAGTTCAACTCGGCCTCTCTTAATCGTCACATTCACCGCA   1645       ADPGPP   CCTACCTCGGCGGG   AAT   ATCAATTTCACTGATGGATCTGTTGAGG         Triticum aestivum     TATTGGCCGCGACGCAAATGCCCGGGGAGGC       Gly162Asn   GCCTCCCCGGGCATTTGCGTCGCGGCCAATACCTCAACAGATCC   1646       GGA-AAT   ATCAGTGAAATTGAT   ATT   CCCGCCGAGGTAGGTGCGGTGAATGTG           ACGATTAAGAGAGGCCGAGTTGAACTGCGTCA           CGGCGGG   AAT   ATCAATT   1647           AATTGAT   ATT   CCCGCCG   1648               Increased Starch   TGACGCAGTTCAACTCGGCCTCTCTTAATCGTCACATTCACCGCA   1649       ADPGPP   CCTACCTCGGCGGG   AAC   ATCAATTTCACTGATGGATCTGTTGAGG         Triticum aestivum     TATTGGCCGCGACGCAAATGCCCGGGGAGGC       Gly162Asn   GCCTCCCCGGGCATTTGCGTCGCGGCCAATACCTCAACAGATCC   1650       GGA-AAC   ATCAGTGAAATTGAT   GTT   CCCGCCGAGGTAGGTGCGGTGAATGTG           ACGATTAAGAGAGGCCGAGTTGAACTGCGTCA           CGGCGGG   AAC   ATCAATT   1651           AATTGAT   GTT   CCCGCCG   1652               Increased Starch   CTTTTCGGAGGAATTATGCTGATCCTAATGAAGTCGCTGCCGTCA   1653       ADPGPP   TTTTGGGTGGTGGTA   AA   GGGACTCAGCTTTTCCCTCTCACAAGCA         Zea mays     CAAGGGCCACCCCTGCTGTTCCTATTGGAGG       Thr96Lys   CCTCCAATAGGAACAGCAGGGGTGGCCCTTGTGCTTGTGAGAGG   1654       ACC-AAA   GAAAAGCTGAGTCCC   TT   TACCACCACCCAAAATGACGGCAGCGAG           TTCATTAGGATCAGCATAATTCCTCCGAAAAG           TGGTGGTA   AA   GGGACTC   1655           GAGTCCC   TT   TACCACCA   1656               Increased Starch   CAAGCACAAGGGCCACCCCTGCTGTTCCTATTGGAGGATGTTACA   1657       ADPGPP   GGCTTATTGATATCC   T   CATGAGCAACTGTTTCAACAGTGGCATAAA         Zea mays     CAAGATATTTGTTATGACTCAGTTCAACTC       Pro124Leu   GAGTTGAACTGAGTCATAACAAATATCTTGTTTATGCCACTGTTGA   1658       CCC-CTC   AACAGTTGCTCATG   A   GGATATCAATAAGCCTGTAACATCCTCCAAT           AGGAACAGCAGGGGTGGCCCTTGTGCTTG           TGATATCC   T   CATGAGCA   1659           TGCTCATG   A   GGATATCA   1660               Increased Starch   TGACTCAGTTCAACTCAGCTTCTCTTAACCGTCACATTCATCGTAC   1661       ADPGPP   CTATCTTGGTGGG   AAT   ATCAACTTCACTGATGGATCTGTTGAGGT         Zea mays     GCTGGCTGCAACACAAATGCCTGGGGAGGC       Gly159Asn   GCCTCCCCAGGCATTTGTGTTGCAGCCAGCACCTCAACAGATCCA   1662       GGG-AAT   TCAGTGAAGTTGAT   ATT   CCCACCAAGATAGGTACGATGAATGTGA           CGGTTAAGAGAAGCTGAGTTGAACTGAGTCA           TGGTGGG   AAT   ATCAACT   1663           AGTTGAT   ATT   CCCACCA   1664               Increased Starch   TGACTCAGTTCAACTCAGCTTCTCTTAACCGTCACATTCATCGTAC   1665       ADPGPP   CTATCTTGGTGGG   AAC   ATCAACTTCACTGATGGATCTGTTGAGGT         Zea mays     GCTGGCTGCAACACAAATGCCTGGGGAGGC       Gly159Asn   GCCTCCCCAGGCATTTGTGTTGCAGCCAGCACCTCAACAGATCCA   1666       GGG-AAC   TCAGTGAAGTTGAT   GTT   CCCACCAAGATAGGTACGATGAATGTGA           CGGTTAAGAGAAGCTGAGTTGAACTGAGTCA           TGGTGGG   AAC   ATCAACT   1667           AGTTGAT   GTT   CCCACCA   1668               Increased Starch   CTTGAGAGGCAAAAGAAGGGCGATGCAAGGACAGTAGTAGCAAT   1669       ADPGPP   CATTCTAGGAGGGGGA   AA   GGGAACTCGTCTTTTCCCCCTCACCAA         Solanum tuberosum     ACGTCGTGCTAAGCCTGCCGTTCCAATGGGAG       Ala58Lys   CTCCCATTGGAACGGCAGGCTTAGCACGACGTTTGGTGAGGGGG   1670       GCG-AAG   AAAAGACGAGTTCCC   TT   TCCCCCTCCTAGAATGATTGCTACTACTG           TCCTTGCATCGCCCTTCTTTTGCCTCTCAAG           GAGGGGGA   AA   GGGAACT   1671           AGTTCCC   TT   TCCCCCTC   1672               Increased Starch   CCAAACGTCGTGCTAAGCCTGCCGTTCCAATGGGAGGAGCATATA   1673       ADPGPP   GGCTAATTGATGTAC   T   AATGAGCAACTGTATTAACAGTGGCATCAA         Solanum tuberosum     CAAAGTATACATTCTCACTCAATTCAACTC       Pro86Leu   GAGTTGAATTGAGTGAGAATGTATACTTTGTTGATGCCACTGTTAA   1674       CCA-CTA   TACAGTTGCTCATT   A   GTACATCAATTAGCCTATATGCTCCTCCCAT           TGGAACGGCAGGCTTAGCACGACGTTTGG           TGATGTAC   T   AATGAGCA   1675           TGCTCATT   A   GTACATCA   1676               Increased Starch   CTCAATTCAACTCAGCCTCACTTAACAGGCATATAGCTCGTGCTTA   1677       ADPGPP   CAACTTTGGCAAT   AAT   GTCACATTCGAGAGTGGCTATGTCGAGGT         Solanum tuberosum     CTTAGCAGCAACTCAAACACCAGGTGAATT       Gly122Asn   AATTCACCTGGTGTTTGAGTTGCTGCTAAGACCTCGACATAGCCA   1678       GGG-AAT   CTCTCGAATGTGAC   ATT   ATTGCCAAAGTTGTAAGCACGAGCTATAT           GCCTGTTAAGTGAGGCTGAGTTGAATTGAG           TGGCAAT   AAT   GTCACAT   1679           ATGTGAC   ATT   ATTGCCA   1680               Increased Starch   CTCAATTCAACTCAGCCTCACTTAACAGGCATATAGCTCGTGCTTA   1681       ADPGPP   CAACTTTGGCAAT   AAC   GTCACATTCGAGAGTGGCTATGTCGAGGT         Solanum tuberosum     CTTAGCAGCAACTCAAACACCAGGTGAATT       Gly122Asn   AATTCACCTGGTGTTTGAGTTGCTGCTAAGACCTCGACATAGCCA   1682       GGG-AAC   CTCTCGAATGTGAC   GTT   ATTGCCAAAGTTGTAAGCACGAGCTATAT           GCCTGTTAAGTGAGGCTGAGTTGAATTGAG           TGGCAAT   AAC   GTCACAT   1683           ATGTGACGTTATTGCCA   1684               Increased Starch   TATTTGAATCTCCAAAAGCTGACCCAAAAAATGTGGCTGCAATTGT   1685       ADPGPP   GCTGGGTGGTGGT   AAA   GGGACTCGCCTCTTTCCTCTTACTAGCAG         Beta vulgaris     GAGAGCTAAGCCAGCAGTGCCAATTGGAGG       Ala98Lys   CCTCCAATTGGCACTGCTGGCTTAGCTCTCCTGCTAGTAAGAGGA   1686       GCT-AAA   AAGAGGCGAGTCCC   TTT   ACCACCACCCAGCACAATTGCAGCCACA           TTTTTTGGGTCAGCTTTTGGAGATTCAAATA           TGGTGGT   AAA   GGGACTC   1687           GAGTCCC   TTT   ACCACCA   1688               Increased Starch   TATTTGAATCTCCAAAAGCTGACCCAAAAAATGTGGCTGCAATTGT   1689       ADPGPP   GCTGGGTGGTGGT   AAC   GGGACTCGCCTCTTTCCTCTTACTAGCAG         Beta vulgaris     GAGAGCTAAGCCAGCAGTGCCAATTGGAGG       Ala98Lys   CCTCCAATTGGCACTGCTGGCTTAGCTCTCCTGCTAGTAAGAGGA   1690       GCT-AAC   AAGAGGCGAGTCCC   GTT   ACCACCACCCAGCACAATTGCAGCCAC           ATTTTTTGGGTCAGCTTTTGGAGATTCAAATA           TGGTGGT   AAC   GGGACTC   1691           GAGTCCC   GTT   ACCACCA   1692               Increased Starch   CTAGCAGGAGAGCTAAGCCAGCAGTGCCAATTGGAGGGTGTTAC   1693       ADPGPP   AGGCTGATTGATGTGC   T   TATGAGCAACTGCATCAACAGTGGCATT         Beta vulgaris     AGAAAGATTTTCATTCTTACCCAGTTCAATTC       Pro126Leu   GAATTGAACTGGGTAAGAATGAAAATCTTTCTAATGCCACTGTTGA   1694       CCT-CTT   TGCAGTTGCTCATA   A   GCACATCAATCAGCCTGTAACACCCTCCAA           TTGGCACTGCTGGCTTAGCTCTCCTGCTAG           TGATGTGC   T   TATGAGCA   1695           TGCTCATA   A   GCACATCA   1696               Increased Starch   CCCAGTTCAATTCGTTTTCGCTTAATCGTCATCTTGCTCGAACCTA   1697       ADPGPP   TAATTTTGGAGAT   AAT   GTGAATTTTGGGGATGGCTTTGTGGAGGTT         Beta vulgaris     TTTGCTGCTACACAAACACCTGGAGAATC       Gly162Asn   GATTCTCCAGGTGTTTGTGTAGCAGCAAAAACCTCCACAAAGCCA   1698       GGT-AAT   TCCCCAAAATTCAC   ATT   ATCTCCAAAATTATAGGTTCGAGCAAGAT           GACGATTAAGCGAAAACGAATTGAACTGGG           TGGAGAT   AAT   GTGAATT   1699           AATTCAC   ATT   ATCTCCA   1700               Increased Starch   CCCAGTTCAATTCGTTTTCGCTTAATCGTCATCTTGCTCGAACCTA   1701       ADPGPP   TAATTTTGGAGAT   AAC   GTGAATTTTGGGGATGGCTTTGTGGAGGT         Beta vulgaris     TTTTGCTGCTACACAAACACCTGGAGAATC       Gly162Asn   GATTCTCCAGGTGTTTGTGTAGCAGCAAAAACCTCCACAAAGCCA   1702       GGT-AAC   TCCCCAAAATTCAC   GTT   ATCTCCAAAATTATAGGTTCGAGCAAGAT           GACGATTAAGCGAAAACGAATTGAACTGGG           TGGAGAT   AAC   GTGAATT   1703           AATTCAC   GTT   ATCTCCA   1704                  
 
     [0142]                   TABLE 21                          Oligonucleotides to produce plants with waxy starch                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               Waxy starch   GAATCCAGGTAAACGGGTAGTTCATAATGGCAACTGTGACTGCTT   1705           GBSS   CTTCTAACTTTGTGT   G   AAGAACTTCACTTTTCAACAATCATGGTGCT         Arabidopsis thaliana     TCTTCATGCTCTGATGTCGCTCAGATTAC       Ser12Term   GTAATCTGAGCGACATCAGAGCATGAAGAAGCACCATGATTGTTG   1706       TCA-TGA   AAAAGTGAAGTTCTT   C   ACACAAAGTTAGAAGAAGCAGTCACAGTTG           CCATTATGAACTACCCGTTTACCTGGATTC           CTTTGTGT   G   AAGAACTT   1707           AAGTTCTT   C   ACACAAAG   1708               Waxy starch   ATCCAGGTAAACGGGTAGTTCATAATGGCAACTGTGACTGCTTCTT   1709       GBSS   CTAACTTTGTGTCA   T   GAACTTCACTTTTCAACAATCATGGTGCTTCT         Arabidopsis thaliana     TCATGCTCTGATGTCGCTCAGATTACCT       Arg13Term   AGGTAATCTGAGCGACATCAGAGCATGAAGAAGCACCATGATTGT   1710       AGA-TGA   TGAAAAGTGAAGTTC   A   TGACACAAAGTTAGAAGAAGCAGTCACAGT           TGCCATTATGAACTACCCGTTTACCTGGAT           TTGTGTCA   T   GAACTTCA   1711           TGAAGTTC   A   TGACACAA   1712               Waxy starch   TAAACGGGTAGTTCATAATGGCAACTGTGACTGCTTCTTCTAACTT   1713       GBSS   TGTGTCAAGAACTTGACTTTTCAACAATCATGGTGCTTCTTCATGCT         Arabidopsis thaliana     CTGATGTCGCTCAGATTACCTTAAAAGG       Ser15Term   CCTTTTAAGGTAATCTGAGCGACATCAGAGCATGAAGAAGCACCAT   1714       TCA-TGA   GATTGTTGAAAAGT   C   AAGTTCTTGACACAAAGTTAGAAGAAGCAGT           CACAGTTGCCATTATGAACTACCCGTTTA           AAGAACTT   G   ACTTTTCA   1715           TGAAAAGT   C   AAGTTCTT   1716               Waxy starch   TGACTGCTTCTTCTAACTTTGTGTCAAGAACTTGACTTTTCAACAAT   1717       GBSS   CATGGTGCTTCTT   G   ATGCTCTGATGTCGCTCAGATTACCTTAAAAG         Arabidopsis thaliana     GCCAATCCTTGACTCATTGTGGGTTAAG       Ser24Term   CTTAACCCACAATGAGTCAAGGATTGGCCTTTTAAGGTAATCTGAG   1718       TCA-TGA   CGACATCAGAGCAT   C   AAGAAGCACCATGATTGTTGAAAAGTGAAG           TTCTTGACACAAAGTTAGAAGAAGCAGTCA           TGCTTCTT   G   ATGCTCTG   1719           CAGAGCAT   C   AAGAAGCA   1720               Waxy starch   TGCTTCTTCTAACTTTGTGTCAAGAACTTCACTTTTCAACAATCATG   1721       GBSS   GTGCTTCTTCATG   A   TCTGATGTCGCTCAGATTACCTTAAAAGGCCA         Arabidopsis thaliana     ATCCTTGACTCATTGTGGGTTAAGGTCA       Cys25Term   TGACCTTAACCCACAATGAGTCAAGGATTGGCCTTTTAAGGTAATC   1722       TGC-TGA   TGAGCGACATCAGATCATGAAGAAGCACCATGATTGTTGAAAAGT           GAAGTTCTTGACACAAAGTTAGAAGAAGCA           TCTTCATG   A   TCTGATGT   1723           ACATCAGA   T   CATGAAGA   1724               Waxy starch   GTAACAGCTTCACAGTTGGTGTCACATGTCCATGGTGGAGCAACG   1725       GBSS   TCTTCACCGGATACT   T   AAACAAACTTGGCCCAGGTTGGCCTCAGG         Antirrhinum majus     AACCAGCAATTCACTCACAATGGGTTGAGAT       Lys24Term   ATCTCAAGCCATTGTGAGTGAATTGCTGGTTCGTGAGGCCAACCTG   1726       AAA-TAA   GGCCAAGTTTGTTT   A   AGTATCGGGTGAAGACGTTGCTCCACCATG           GACATGTGACACCAACTGTGAAGGTGTTAC           CGGATACT   T   AAACAAAC   1727           GTTTGTTT   A   AGTATCCG   1728               Waxy starch   CACAGTTGGTGTCACATGTCCATGGTGGAGCAAGGTCTTCACCGG   1729       GBSS   ATAGTAAAACAAACT   A   GGGCGAGGTTGGCCTCAGGAACCAGCAAT         Antirrhinum majus     TCACTCACAATGGGTTGAGATCAATAAACAT       Leu27Term   ATGTTTATTGATCTCAACCCATTGTGAGTGAATTGCTGGTTCCTGA   1730       TTG-TAG   GGCCAACCTGGGCC   T   AGTTTGTTTTAGTATCGGGTGAAGACGTTG           CTCCACCATGGACATGTGACACCAACTGTG           AACAAACT   A   GGCCCAGG   1731           CCTGGGCC   T   AGTTTGTT   1732               Waxy starch   TTGGTGTCACATGTCCATGGTGGAGCAACGTCTTCACCGGATACT   1733       GBSS   AAAACAAACTTGGCC   T   AGGTTGGCCTCAGGAACCAGCAATTCACT         Antirrhinum majus     CACAATGGGTTGAGATCAATAAACATGGTTG       Gln29Term   CAACCATGTTTATTGATCTCAACCCATTGTGAGTGAATTGCTGGTT   1734       GAG-TAG   CCTGAGGCCAACCT   A   GGCCAAGTTTGTTTTAGTATCCGGTGAAGA           CGTTGCTCCACCATGGACATGTGACACCAA           ACTTGGCC   T   AGGTTGGC   1735           GCCAACCT   A   GGCCAAGT   1736               Waxy starch   GGTGGAGCAACGTCTTCACCGGATACTAAAACAAACTTGGCCCAG   1737       GBSS   GTTGGCCTCAGGAACTAGCAATTCACTCACAATGGGTTGAGATCA         Antirrhinum majus     ATAAACATGGTTGATAAGCTTCAAATGAGGA       Gln35Term   TCCTCATTTGAAGCTTATCAACCATGTTTATTGATGTCAACCCATTG   1738       GAG-TAG   TGAGTGAATTGCT   A   GTTCCTGAGGCCAACCTGGGCCAAGTTTGTTT           TAGTATCCGGTGAAGACGTTGCTCCACC           TCAGGAAC   T   AGCAATTC   1739           GAATTGCT   A   GTTCCTGA   1740               Waxy starch   GGAGCAACGTCTTCACCGGATACTAAAACAAACTTGGCCCAGGTT   1741       GBSS   GGCCTCAGGAACCAG   T   AATTCACTCACAATGGGTTGAGATCAATAA         Antirrhinum majus     ACATGGTTGATAAGCTTCAAATGAGGAACA       Gln36Term   TGTTCCTCATTTGAAGCTTATCAACCATGTTTATTGATCTCAACCCA   1742       CAA-TAA   TTGTGAGTGAATT   A   CTGGTTCCTGAGGCCAACCTGGGCCAAGTTT           GTTTTAGTATCCGGTGAAGACGTTGCTCC           GGAACCAG   T   AATTCACT   1743           AGTGAATT   A   CTGGTTCC   1744               Waxy starch   GTGATGGCGACTATAACTGCCTCACACTTTGTTTCTCATGTCTGTG   1745       GBSS   GGGGTGCCACTTCT T GAGAATCAAAAGTGGGGTTGGGTCAATTAG         Ipomoea batatas     CCCTGAGGAGCCAAGCTGTGACTCACAATG       Gly20Term   CATTGTGAGTCACAGCTTGGCTCCTCAGGGCTAATTGACCCAACC   1746       GGA-TGA   CCACTTTTGATTCTC   A   AGAAGTGGCACCCCCACAGACATGAGAAA           CAAAGTGTGAGGCAGTTATAGTCGCCATCAC           CCACTTCT   T   GAGAATCA   1747           TGATTCTC   A   AGAAGTGG   1748               Waxy starch   ATGGCGACTATAACTGCCTCACACTTTGTTTCTCATGTCTGTGGGG   1749       GBSS   GTGCCACTTCTGGA   T   AATCAAAAGTGGGGTTGGGTCAATTAGCCC         Ipomoea batatas     TGAGGAGCCAAGCTGTGACTCACAATGGGT       Glu21Term   ACCCATTGTGAGTCACAGCTTGGCTCCTCAGGGCTAATTGACCCA   1750       GAA-TAA   ACCCCACTTTTGATT   A   TCCAGAAGTGGCACCCCCACAGACATGAG           AAACAAAGTGTGAGGCAGTTATAGTCGCCAT           CTTCTGGA   T   AATCAAAA   1751           TTTTGATT   A   TCCAGAAG   1752               Waxy starch   CGACTATAACTGCCTCACACTTTGTTTCTCATGTCTGTGGGGGTGC   1753       GBSS   CACTTCTGGAGAAT   G   AAAAGTGGGGTTGGGTCAATTAGCCCTGAG         Ipomoea batatas     GAGCCAAGCTGTGACTCACAATGGGTTGAG       Ser22Term   CTCAACCCATTGTGAGTCACAGCTTGGCTCCTCAGGGCTAATTGA   1754       TCA-TGA   CCCAACCCCACTTTT   C   ATTCTCCAGAAGTGGCACCCCCACAGACAT           GAGAAACAAAGTGTGAGGCAGTTATAGTCG           TGGAGAAT   G   AAAAGTGG   1755           CCACTTTT   C   ATTCTCCA   1756               Waxy starch   ACTATAACTGCCTCACACTTTGTTTCTCATGTCTGTGGGGGTGCCA   1757       GBSS   CTTCTGGAGAATCA   T   AAGTGGGGTTGGGTCAATTAGCCCTGAGGA         Ipomoea batatas     GCCAAGCTGTGACTCACAATGGGTTGAGAC       Lys23Term   GTCTCAACCCATTGTGAGTCACAGCTTGGCTCCTCAGGGCTAATT   1758       AAA-TAA   GACCCAACCCCACTT   A   TGATTCTCCAGAAGTGGCACCCCCACAGA           CATGAGAAACAAAGTGTGAGGCAGTTATAGT           GAGAATCA   T   AAGTGGGG   1759           CCCCACTT   A   TGATTCTC   1760               Waxy starch   CCTCACACTTTGTTTCTCATGTCTGTGGGGGTGCCACTTCTGGAGA   1761       G BSS   ATCAAAAGTGGGGT   A   GGGTCAATTAGCCCTGAGGAGCCAAGCTGT         Ipomoea batatas     GACTCACAATGGGTTGAGACCTGTGAACAA       Leu26Term   TTGTTCACAGGTCTCAACCCATTGTGAGTCACAGCTTGGCTCCTCA   1762       TTG-TAG   GGGCTAATTGACCC   T   ACCCCACTTTTGATTCTCCAGAAGTGGCACC           CCCACAGACATGAGAAACAAAGTGTGAGG           AGTGGGGT   A   GGGTCAAT   1763           ATTGACCC   T   ACCCCACT   1764               Waxy starch   CATCGGCGATTGTTGCTCCTTACTGCTCTCTCACAGAATGGCAACG   1765       GBSS   GTGACGGGGTCTTA   G   GTGGTGTCGAGAAGCGCGTGCTTCAATTCC         Astragalus     CAGGGAAGAACAGAAGCCAAAGTGAATTCA         membranaeus     TGAATTCACTTTGGCTTCTGTTCTTCCCTGGGAATTGAAGCACGCG   1766       Tyr8Term   CTTCTCGACACCAC   C   TAAGACCCCGTCACCGTTGCCATTCTGTGA       TAT-TAG   GAGAGCAGTAAGGAGCAACAATCGCCGATG           GGGTCTTA   G   GTGGTGTC   1767           GACACCAC   C   TAAGACCC   1768               Waxy starch   ATTGTTGCTCCTTACTGCTCTCTCACAGAATGGCAACGGTGACGG   1769       GBSS   GGTCTTATGTGGTGT   A   GAGAAGCGCGTGCTTCAATTCCCAGGGAA         Astragalus     GAACAGAAGCCAAAGTGAATTCACCTCAGAA         membranaeus     TTCTGAGGTGAATTCACTTTGGCTTCTGTTCTTCCCTGGGAATTGA   1770       Ser11Term   AGCACGCGCTTCTCTACACCACATAAGACCCCGTCACCGTTGCCA       TCG-TAG   TTCTGTGAGAGAGCAGTAAGGAGCAACAAT           TGTGGTGT   A   GAGAAGCG   1771           CGCTTCTC   T   ACACCACA   1772               Waxy starch   TGTTGCTCCTTACTGCTCTCTCACAGAATGGCAACGGTGACGGGG   1773       GBSS   TCTTATGTGGTGTCGTGAAGCGCGTGCTTCAATTCCCAGGGAAGA         Astragalus     ACAGAAGCCAAAGTGAATTCACCTCAGAAGA         membranaeus     TCTTCTGAGGTGAATTCACTTTGGCTTCTGTTCTTCCCTGGGAATT   1774       Arg12Term   GAAGCACGCGCTTC   A   CGACACCACATAAGACCCCGTCACCGTTGC       AGA-TGA   CATTCTGTGAGAGAGCAGTCAGGAGCAACA           TGGTGTCG   T   GAAGCGCG   1775           CGCGCTTC   A   CGACACCA   1776               Waxy starch   ACTGCTCTCTCACAGAATGGCAACGGTGACGGGGTCTTATGTGGT   1777       GBSS   GTCGAGAAGCGCGTG   A   TTCAATTCCCAGGGAAGAACAGAAGCCAA         Astragalus     AGTGAATTCACCTCAGAAGATAAATCTGAAT         membranaeus     ATTGAGATTTATCTTCTGAGGTGAATTCACTTTGGCTTCTGTTCTTC   1778       Cys15Term   CCTGGGAATTGAATCACGCGCTTCTCGACACCACATAAGACCCCG       TGC-TGA   TCACCGTTGCCATTCTGTGAGAGAGCAGT           AGCGCGTG   A   TTCAATTC   1779           GAATTGAA   T   CACGCGCT   1780               Waxy starch   CACAGAATGGCAACGGTGACGGGGTCTTATGTGGTGTCGAGAAG   1781       GBSS   CGCGTGGTTCAATTCCTAGGGAAGAACAGAAGCCAAAGTGAATTC         Astragalus     ACCTCAGAAGATAAATCTCAATAGCCAAGCAT         membranaeus     ATGCTTGGCTATTGAGATTTATCTTCTGAGGTGAATTCACTTTGGCT   1782       Gln19Term   TCTGTTCTTCCCTAGGAATTGAAGCACGCGCTTCTCGACACCACAT       CAG-TAG   AAGACCCCGTCACCGTTGCCATTCTGTG           TCAATTCC   T   AGGGAAGA   1783           TCTTCCCT   A   GGAATTGA   1784               Waxy starch   TGTAGCTTGGTAGATTCCCCTTTTTGTCGACCACACATCACATGGC   1785       GBSS   AAGCATCACAGCTT   G   ACACCACTTTGTGTCAAGAAGCCAAACTTCA         Solanum tuberosum     CTAGACACCAAATCAACCTTGTCACAGAT       Ser7Term   ATCTGTGACAAGGTTGATTTGGTGTCTAGTGAAGTTTGGCTTCTTG   1786       TCA-TGA   ACACAAAGTGGTGT   C   AAGCTGTGATGCTTGCCATGTGATGTGTGG           TCTACAAAAAGGGGAATCTACCAAGCTACA           CACAGCTT   G   ACACCACT   1787           AGTGGTGT   C   AAGCTGTG   1788               Waxy starch   TCCCCTTTTTGTAGACCACACATCACATGGCAAGCATCACAGCTTC   1789       GBSS   ACACCACTTTGTGT   G   AAGAAGCCAAACTTCACTAGACACCAAATCA         Solanum tuberosum     ACCTTGTCACAGATAGGACTCAGGAACCA       Ser12Term   TGGTTCCTGAGTCCTATCTGTGACAAGGTTGATTTGGTGTCTAGTG   1790       TCA-TGA   AAGTTTGGCTTCTT   C   ACACAAAGTGGTGTGAAGCTGTGATGCTTGC           CATGTGATGTGTGGTCTACAAAAAGGGGA           CTTTGTGT   G   AAGAAGCC   1791           GGCTTCTT   C   ACACAAAG   1792               Waxy starch   CCCTTTTTGTAGACCACACATCACATGGCAAGCATCACAGCTTCAC   1793       GBSS   ACCACTTTGTGTCATGAAGCCAAACTTCACTAGACACCAAATCAAC         Solanum tuberosum     CTTGTCACAGATAGGACTCAGGAACCATA       Arg13Term   TATGGTTCCTGAGTCCTATCTGTGACAAGGTTGATTTGGTGTCTAG   1794       AGA-TGA   TGAAGTTTGGCTTC   A   TGACACAAAGTGGTGTGAAGCTGTGATGCTT           GCCATGTGATGTGTGGTCTACAAAAAGGG           TTGTGTCA   T   GAAGCCAA   1795           TTGGCTTC   A   TGACACAA   1796               Waxy starch   TTGTAGACCACACATCACATGGCAAGCATCACAGCTTCACACCACT   1797       GBSS   TTGTGTCAAGAAGCTAAACTTCACTAGACACCAAATCAACCTTGTC         Solanum tuberosum     ACAGATAGGACTCAGGAACCATACTCTGA       Gln15Term   TCAGAGTATGGTTCCTGAGTCCTATCTGTGACAAGGTTGATTTGGT   1798       CAA-TAA   GTCTAGTGAAGTTT   A   GCTTCTTGACACAAAGTGGTGTGAAGCTGTG           ATGCTTGCCATGTGATGTGTGGTCTACAA           CAAGAAGC   T   AAACTTCA   1799           TGAAGTTT   A   GCTTCTTG   1800               Waxy starch   CCACACATCACATGGCAAGCATCACAGCTTCACACCACTTTGTGTC   1801       GBSS   AAGAAGCCAAACTT   G   ACTAGACACCAAATCAACCTTGTCACAGATA         Solanum tuberosum     GGACTCAGGAACCATACTCTGACTCACAA       Sen17Term   TTGTGAGTCAGAGTCTGGTTCCTGAGTCCTATCTGTGACAAGGTTG   1802       TCA-TGA   ATTTGGTGTCTAGT   C   AAGTTTGGCTTGTTGACACAAAGTGGTGTGA           AGCTGTGATGCTTGCCATGTGATGTGTGG           CCAAACTT   G   ACTAGACA   1803           TGTCTAGT   C   AAGTTTGG   1804               Waxy starch   GTCGATCACTCTTCTCTCACCGCCGAAACAGATTTTGACACAAAAA   1805       GBSS   TGGCAACAATAACG   T   GATCTTCAATGCCGACGAGAACCGCGTGCT         Pisum sativum     TCAATTACCAAGGAAGATCAGCAGAGTCTA       Gly6Term   TAGACTCTGCTGATCTTCCTTGGTCATTGAAGCACGCGGTTCTCGT   1806       GGA-TGA   CGGCATTGAAGATC   A   CGTTATTGTTGCCATTTTTGTGTCAAAATCT           GTTTCGGCGGTGAGAGAAGAGTGATCGAC           CAATAACG   T   GATCTTCA   1807           TGAAGATC   A   CGTTATTG   1808               Waxy starch   ACTCTTCTCTCACCGCCGAAACAGATTTTGACACAAAAATGGCAAC   1809       GBSS   AATAACGGGATCTT   G   AATGCCGACGAGAACCGCGTGCTTCAATTA         Pisum sativum     CCAAGGAAGATCAGCAGAGTCTAAACTGAA       Ser8Term   TTCAGTTTAGACTCTGCTGATCTTCCTTGGTCATTGAAGCACGCGG   1810       TCA-TGA   TTCTCGTCGGCATT   C   AAGATCCCGTTATTGTTGCCATTTTTGTGTCA           AAATCTGTTTCGGCGGTGAGAGAAGAGT           GGGATCTT   G   AATGCCGA   1811           TCGGCATT   C   AAGATCCC   1812               Waxy starch   ACCGCCGAAACAGATTTTGACACAAAAATGGCAACAATAACGGGA   1813       GBSS   TCTTCAATGCCGACG   T   GAACCGCGTGCTTCAATTACCAAGGAAGA         Pisum sativum     TCAGCAGAGTCTAAACTGAATTTGCCTCAGA       Arg12Term   TCTGAGGCAAATTCAGTTTAGACTCTGCTGATCTTCCTTGGTCATT   1814       AGA-TGA   GAAGCACGCGGTTC   A   CGTCGGCATTGAAGATCCCGTTATTGTTGC           CATTTTTGTGTCAAAATCTGTTTCGGCGGT           TGCCGACG   T   GAACCGCG   1815           CGCGGTTC   A   CGTCGGCA   1816               Waxy starch   AGATTTTGACACAAAAATGGCAACAATAACGGGATCTTCAATGCCG   1817       GBSS   ACGAGAACCGCGTG   A   TTCAATTACCAAGGAAGATCAGCAGAGTCT         Pisum sativum     AAACTGAATTTGCCTCAGATACACTTCAAT       Cys15Term   ATTGAAGTGTCTCTGAGGCAAATTCAGTTTAGACTCTGCTGATCTT   1818       TGC-TGA   CCTTGGTCATTGAA   T   CACGCGGTTCTCGTCGGCATTGAAGATCCC           GTTATTGTTGCCATTTTTGTGTCAAAATCT           ACCGCGTG   A   TTCAATTA   1819           TAATTGAA   T   CACGCGGT   1820               Waxy starch   CACAAAAATGGCAACAATAACGGGATCTTCAATGCCGACGAGAAC   1821       GBSS   CGCGTGCTTCAATTA   G   CAAGGAAGATCAGCAGAGTCTAAACTGAA         Pisum sativum     TTTGCCTCAGATACACTTCAATAACAACCAA       Tyr18Term   TTGGTTGTTATTGAAGTGTATCTGAGGCAAATTCAGTTTAGACTCT   1822       TAC-TAG   GCTGATCTTCCTTG   C   TAATTGAAGCACGCGGTTCTCGTCGGCATTG           AAGATCCCGTTATTGTTGCCATTTTTGTG           TTCAATTA   G   CAAGGAAG   1823           CTTCCTTG   C   TAATTGAA   1824               Waxy starch   TCTACACCGGAGAGAGCACCATGGCAACTGTAATAGCTGCACATT   1825       GBSS   TCGTTTCCAGGAGCT   G   ACACTTGAGCATCCATGCATTAGAGACTAA         Manihot esculenta     GGCTAATAATTTGTCTCACACTGGACCCTG       Ser14Term   CAGGGTCCAGTGTGAGACAAATTATTAGCCTTAGTCTCTAATGCAT   1826       TCA-TGA   GGATGCTCAAGTGT   C   AGCTCCTGGAAACGAAATGTGCAGCTATTA           CAGTTGCCATGGTGCTCTCTCCGGTGTAGA           CAGGAGCT   G   ACACTTGA   1827           TCAAGTGT   C   AGCTCCTG   1828               Waxy starch   CCGGAGAGAGCACCATGGCAACTGTAATAGCTGCACATTTCGTTT   1829       GBSS   CCAGGAGCTCACACT   A   GAGCATCCATGCATTAGAGACTAAGGCTA         Manihot esculenta     ATAATTTGTCTCACACTGGACCCTGGACCCA       Leu16Term   TGGGTCCAGGGTCCAGTGTGAGACAAATTATTAGCCTTAGTCTCTA   1830       TTG-TAG   ATGCATGGATGCTC   T   AGTGTGAGCTCCTGGAAACGAAATGTGCAG           CTATTACAGTTGCCATGGTGCTCTCTCCGG           CTCACACT   A   GAGCATCC   1831           GGATGCTC   T   AGTGTGAG   1832               Waxy starch   TGGCAACTGTAATAGCTGCACATTTCGTTTCCAGGAGCTCACACTT   1833       GBSS   GAGCATCCATGCAT   G   AGAGACTAAGGCTAATAATTTGTCTCACACT         Manihot esculenta     GGACCCTGGACCCAAACTATCACTCCCAA       Leu21Term   TTGGGAGTGATAGTTTGGGTCCAGGGTCCAGTGTGAGACAAATTA   1834       TTA-TGA   TTAGCCTTAGTCTCT   C   ATGCATGGATGCTCAAGTGTGAGCTCCTGG           AAACGAAATGTGCAGCTATTACAGTTGCCA           CCATGCAT   G   AGAGACTA   1835           TAGTCTCT   C   ATGCATGG   1836               Waxy starch   GCAACTGTAATAGCTGCACATTTCGTTTCCAGGAGCTCACACTTGA   1837       GBSS   GCATCCATGCATTA   T   AGACTAAGGCTAATAATTTGTCTCACACTGG         Manihot esculenta     ACCCTGGACCCAAACTATCACTCCCAATG       Glu22Term   CATTGGGAGTGATAGTTTGGGTCCAGGGTCCAGTGTGAGACAAAT   1838       GAG-TAG   TATTAGCCTTAGTCT   A   TAATGCATGGATGCTCAAGTGTGAGCTCCT           GGAAACGAAATGTGCAGCTATTACAGTTGC           ATGCATTA   T   AGACTAAG   1839           CTTAGTCT   A   TAATGCAT   1840               Waxy starch   GTCATAGCTGCACATTTCGTTTCCAGGAGCTCACACTTGAGCATCC   1841       GBSS   ATGCATTAGAGACT   T   AGGCTAATAATTTGTCTCACACTGGACCCTG         Manihot esculenta     GACCCAAACTATCACTCCCAATGGTTTAA       Lys24Term   TTAAACCATTGGGAGTGATAGTTTGGGTCCAGGGTCCAGTGTGAG   1842       AAG-TAG   ACAAATTATTAGCCT   A   AGTCTCTAATGCATGGATGCTCAAGTGTGA           GCTCCTGGAAACGAAATGTGCAGCTATTAC           TAGAGACT   T   AGGCTAAT   1843           ATTAGCCT   A   AGTCTCTA   1844               Waxy starch   ACAACTCCTCCGTCACCGGTATAAGCATGGCAACGGTATCGATGG   1845       GBSS   CATCGTGCGTGGCGT   G   AAAAGGCGCGTGGAGTACAGAGACAAAA         Phaseolus vulgaris     GTGAAATCTTCGGGTCAGATGAGCCTGAACCG       Ser12Term   CGGTTCAGGCTCATCTGACCCGAAGATTTCACTTTTGTCTCTGTCC   1846       TCA-TGA   TCCACGCGCCTTTT   C   ACGCCACGCACGATGCCATCGATACCGTTG           CCATGCTTATACCGGTGACGGAGGAGTTGT           CGTGGCGT   G   AAAAGGCG   1847           CGCCTTTT   C   ACGCCACG   1848               Waxy starch   CACCGGTCTAAGCATGGCAACGGTATCGATGGCATCGTGCGTGGC   1849       GBSS   GTCAAAAGGCGCGTG   A   AGTACAGAGACAAAAGTGAAATCTTCGGG         Phaseolus vulgaris     TCAGATGAGCCTGAACCGTCATGAATTGAAA       Trp16Term   TTTCAATTCATGACGGTTCAGGCTCATCTGACCCGAAGATTTCACT   1850       TGG-TGA   TTTGTCTCTGTACT   T   CACGCGCCTTTTGACGCCACGCACGATGCCA           TCGATACCGTTGGCATGCTTATACCGGTG           GGCGCGTG   A   AGTACAGA   1851           TCTGTACT   T   CACGCGCC   1852               Waxy starch   ATAAGCATGGCAACGGTCTCGATGGCATCGTGCGTGGCGTCAAAA   1853       GBSS   GGCGCGTGGAGTACA   T   AGACAAAAGTGAAATCTTCGGGTCAGATG         Phaseolus vulgaris     AGCCTGAACCGTCATGAATTGAAATACGATG       Glu19Term   CATCGTATTTCAATTCATGACGGTTCAGGCTCATCTGACCCGAAGA   1854       GAG-TAG   TTTCACTTTTGTCT   A   TGTACTCCACGCGCCTTTTGACGCCACGCAC           GATGCCATCGATACCGTTGCCATGCTTAT           GGAGTACA   T   AGACAAAA   1855           TTTTGTCT   A   TGTACTCC   1856               Waxy starch   ATGGCAACGGTATCGATGGCATCGTGCGTGGGGTCAAAAGGCGC   1857       GBSS   GTGGAGTACAGAGACA   T   AAGTGAAATCTTCGGGTCAGATGAGCCT         Phaseolus vulgaris     GAACCGTCATGAATTGAAATACGATGGGTTGA       Lys21Term   TCAACCCATCGTATTTCAATTCATGACGGTTCAGGCTCATCTGACC   1858       AAA-TAA   CGAAGATTTCACTT   A   TGTCTCTGTACTCCACGCGCCTTTTGACGCC           ACGCACGATGCCATCGATACCGTTGCCAT           CAGAGACA   T   AAGTGAAA   1859           TTTCACTT   A   TGTCTCTG   1860               Waxy starch   ACGGTATCGATGGCATCGTGCGTGGCGTCAAAAGGCGCGTGGAG   1861       GBSS   TACAGAGACAAAAGTG   T   AATCTTCGGGTCAGATGAGCCTGAACCG         Phaseolus vulgaris     TCATGAATTGAAATACGATGGGTTGAGATCTC       Lys23Term   GAGATCTCAACCCATCGTATTTCAATTCATGACGGTTCAGGCTCAT   1862       AAA-TAA   CTGACCCGAAGATT   A   CACTTTTGTCTCTGTACTCCACGCGCCTTTT           GACGCCACGCACGATGCCATCGATACCGT           CAAAAGTG   T   AATCTTCG   1863           CGAAGATT   A   CACTTTTG   1864               Waxy starch   GCGCCTAGCTCGAAAAGGTCGTCATTGAGAGGCTGCACCAATGG   1865       GBSS   GTTCCATTCCTAATTA   G   TGTTCTTATCAAACAAACAGTGTTGGTTCA         Triticum aestivum     CTGAAACTGTCGCCTCACATCCAATTCCAG       Tyr7Term   CTGGAATTGGATGTGAGGCGACAGTTTCAGTGAACCAACACTGTT   1866       TAT-TAG   TGTTTGATAAGAACA   C   TAATTAGGAATGGAACCCATTGGTGCAGCC           TCTCAATGACGACCTTTTCGAGCTAGGCGC           CCTAATTA   G   TGTTCTTA   1867           TAAGAACA   C   TAATTAGG   1868               Waxy starch   CCTAGCTCGAAAAGGTCGTCATTGAGAGGCTGCACCAATGGGTTC   1869       GBSS   CATTCCTAATTATTG   A   TCTTATCAAACAAACAGTGTTGGTTCACTGA         Triticum aestivum     AACTGTCGCCTCACATCCAATTCCAGCAA       Cys8Term   TTGCTGGAATTGGATGTGAGGCGACAGTTTCAGTGAACCAACACT   1870       TGT-TGA   GTTTGTTTGATAAGA   T   CAATAATTAGGAATGGAACCCATTGGTGCA           GCCTCTCAATGACGACCTTTTCGAGCTAGG           AATTATTG   A   TCTTATCA   1871           TGATAAGA   T   CAATAATT   1872               Waxy starch   TCGAAAAGGTCGTCATTGAGAGGCTGCACCAATGGGTTCCATTCC   1873       GBSS   TAATTATTGTTCTTA   G   CAAACAAACAGTGTTGGTTCACTGAAACTGT         Triticum aestivum     CGCCTCACATCCAATTCCAGCAATCTTGT       Tyr10Term   ACAAGATTGCTGGAATTGGATGTGAGGCGACAGTTTCAGTGAACC   1874       TAT-TAG   AACACTGTTTGTTTG   C   TAAGAACAATAATTAGGAATGGAACCCATT           GGTGCAGCCTCTCAATGACGACCTTTTCGA           TGTTCTTA   G   CAAACAAA   1875           TTTGTTTG   C   TAAGAACA   1876               Waxy starch   CGAAAAGGTCGTCATTGAGAGGCTGCACCAATGGGTTCCATTCCT   1877       GBSS   AATTATTGTTCTTAT   T   AAACAAACAGTGTTGGTTCACTGAAACTGTC         Triticum aestivum     GCCTCACATCCAATTCCAGCAATCTTGTA       Gln11Term   TACAAGATTGCTGGAATTGGATGTGAGGCGACAGTTTCAGTGAAC   1878       CAA-TAA   CAACACTGTTTGTTT   A   ATAAGAACAATAATTAGGAATGGAACCCATT           GGTGCAGCCTCTCAATGACGACCTTTTCG           GTTCTTAT   T   AAACAAAC   1879           GTTTGTTT   A   ATAAGAAC   1880               Waxy starch   AGGCTGCACCAATGGGTTCCATTCCTAATTATTGTTCTTATCAAACA   1881       GBSS   AACAGTGTTGGTT   G   ACTGAAACTGTCGCCTCACATCCAATTCCAGC         Triticum aestivum     AATCTTGTCACAATGAAGTTATGTTCCT       Ser17Term   AGGAACATAACTTCATTGTTACAAGATTGCTGGAATTGGATGTGAG   1882       TCA-TGA   GCGACAGTTTCAGT   C   AACCAACACTGTTTGTTTGATAAGAACAATA           ATTAGGAATGGAACCCATTGGTGCAGCCT           TGTTGGTT   G   ACTGAAAC   1883           GTTTCAGT   C   AACCAACA   1884               Waxy starch   CAGCTCGCCACCTCCGGCACCGTCCTCGGCATCACCGACAGGTT   1885       GBSS   CCGGCGTGCAGGTTTC   T   AGGGCGTGAGGCCCCGGAGCCCGGCG         Triticum aestivum     GATGCGGCTCTCGGCATGAGGACCGTCGGAGCTA       Gln28Term   TAGCTCCGACGGTCCTCATGCCGAGAGCCGCATCCGCCGGGCTC   1886       CAG-TAG   CGGGGCCTCACGCCCT   A   GAAACCTGCACGCCGGAACCTGTCGGT           GATGCCGAGGACGGTGCCGGAGGTGGCGAGCTG           CAGGTTTC   T   AGGGCGTG   1887           CACGCCCT   A   GAAACCTG   1888               Waxy starch   GGTTTCCAGGGCGTGAGGCCCCGGAGCCCGGCGGATGCGGCTCT   1889       GBSS   CGGCATGAGGACCGTC   T   GAGCTAGCGCCGCCCCAACGCAAAGCC         Triticum aestivum     GGAAAGCGCACCGCGGGACCCGGCGGTGCCTCT       Gly46Term   AGAGGCACCGCCGGGTCCCGCGGTGCGCTTTCCGGCTTTGCGTT   1890       GGA-TGA   GGGGCGGCGCTAGCTC   A   GACGGTCCTCATGCCGAGAGCCGCATC           CGCCGGGCTCCGGGGCCTCACGCCCTGGAAACC           GGACCGTC   T   GAGCTAGC   1891           GCTAGCTC   A   GACGGTCC   1892               Waxy starch   CGGAGCCCGGCGGATGCGGCTCTCGGCATGAGGACCGTCGGAG   1893       GBSS   CTAGCGCCGCCCCAACG   T   AAAGCCGGAAAGCGCACCGCGGGACC         Triticum aestivum     CGGCGGTGCCTCTCCATGGTGGTGCGCGCCACCG       Gln53Term   CGGTGGCGCGCACCACCATGGAGAGGCACCGCCGGGTCCCGCG   1894       CAA-TAA   GTGCGCTTTCCGGCTTT   A   CGTTGGGGCGGCGCTAGCTCCGACGG           TCCTCATGCCGAGAGCCGCATCCGCCGGGCTCCG           CCCCAACG   T   AAAGCCGG   1895           CCGGCTTT   A   CGTTGGGG   1896               Waxy starch   GCGGATGCGGCTCTCGGCATGAGGACCGTCGGAGCTAGCGCCGC   1897       GBSS   CCCAACGCAAAGCCGG   T   AAGCGCACCGCGGGACCCGGCGGTGC         Triticum aestivum     CTCTCCATGGTGGTGCGCGCCACCGGCAGCGGCG       Lys56Term   CGCCGCTGCCGGTGGCGCGCACCACCATGGAGAGGCACCGCCG   1898       AAA-TAA   GGTCCCGCGGTGCGCTT   A   CCGGCTTTGCGTTGGGGCGGCGCTAG           CTCCGACGGTCCTCATGCCGAGAGCCGCATCCGC           AAAGCCGG   T   AAGCGCAC   1899           GTGCGCTT   A   CCGGCTTT   1900               Waxy starch   CTCTCCATGGTGGTGCGCGCCACCGGCAGCGGCGGCATGAACCT   1901       GBSS   CGTGTTCGTCGGCGCC   T   AGATGGCGCCCTGGACCAAGACCGGCG         Triticum aestivum     GCCTCGGCGACGTCCTCGGGGGCCTCCCCCCAG       Glu85Term   CTGGGGGGAGGCCCCCGAGGACGTCGCCGAGGCCGCCGGTCTT   1902       GAG-TAG   GCTCCAGGGCGCCATCT   A   GGCGCCGACGAACACGAGGTTCATGC           CGCCGCTGCCGGTGGCGCGCACCACCATGGAGAG           TCGGCGCC   T   AGATGGCG   1903           CGCCATCT   A   GGCGCCGA   1904               Waxy starch   GTGGTCTCTCGCTGCAGGTAGCCACACCCTGCGCGCGCGATGGC   1905       GBSS   GGCTCTGGTCACGTCG   T   AGCTCGCCACCTCCGGCACCGTCCTCG         Triticum aestivum     GCATCACCGACAGGTTCCGGCGTGCAGGTTTTC       Gln8Term   GAAAACCTGCACGCCGGAACCTGTCGGTGATGCCGAGGACGGTG   1906       CAG-TAG   CCGGAGGTGGCGAGCT   A   CGACGTGACCAGAGCCGCCATCGCGC           GCGCAGGGTGTGGCTACCTGCAGCGAGAGACGAC           TCACGTCG   T   AGCTCGCC   1907           GGCGAGCT   A   CGACGTGA   1908               Waxy starch   CAGCTCGCCACCTCCGGCACCGTCCTCGGCATCACCGACAGGTT   1909       GBSS   CCGGCGTGCAGGTTTT   T   AGGGTGTGAGGCCCCGGAGCCCGGCAG         Triticum aestivum     ATGCGCCGCTCGGCATGAGGACTACCGGAGCGA       Gln28Term   TCGCTCCGGTCGTCCTCATGCCGAGCGGCGCATCTGCCGGGCTC   1910       GAG-TAG   CGGGGCCTCACACCCT   A   AAAACCTGCACGCCGGAACCTGTCGGT           GATGCCGAGGACGGTGCCGGAGGTGGCGAGCTG           CAGGTTTT   T   AGGGTGTG   1911           CACACCCT   A   AAAACCTG   1912               Waxy starch   CCCCGGAGCCCGGCAGATGCGCCGCTCGGCATGAGGACTACCGG   1913       GBSS   AGCGAGCGCCGCCCCG   T   AGCAACAAAGCCGGAAAGCGCACCGCG         Triticum aestivum     GGACCCGGCGGTGCCTCTCCATGGTGGTGCGCG       Lys52Term   CGCGCACCACCATGGAGAGGCACCGCCGGGTCCCGCGGTGCGC   1914       AAG-TAG   TTTCCGGCTTTGTTGCT   A   CGGGGCGGCGCTCGCTCCGGTAGTCCT           CATGCCGAGCGGCGCATCTGCCGGGCTCCGGGG           CCGCCCCG   T   AGCAACAA   1915           TTGTTGCT   A   CGGGGCGG   1916               Waxy starch   CGGAGCCCGGCAGATGCGCCGCTCGGCATGAGGACTACCGGAG   1917       GBSS   CGAGCGCCGCCCCGAAG   T   AACAAAGCCGGAAAGCGCACCGCGG         Triticum aestivum     GACCCGGCGGTGCCTCTCCATGGTGGTGCGCGCCA       Gln53Term   TGGCGCGCACCACCATGGAGAGGCACCGCCGGGTCCCGCGGTG   1918       CAA-TAA   CGCTTTCCGGCTTTGTT   A   CTTCGGGGCGGCGCTCGCTCCGGTAGT           CCTCATGCCGAGCGGCGCATCTGCCGGGCTCCG           CCCCGAAG   T   AACAAAGC   1919           GCTTTGTT   A   CTTCGGGG   1920               Waxy starch   AGCCCGGCAGATGCGCCGCTCGGCATGAGGACTACCGGAGCGAG   1921       GBSS   CGCCGCCCCGAAGCAA   T   AAAGCCGGAAAGCGCACCGCGGGACCC         Triticum aestivum     GGCGGTGCCTCTCCATGGTGGTGCGCGCCACGG       Gln54Term   CCGTGGCGCGCACCACCATGGAGAGGCACCGCCGGGTCCCGCG   1922       CAA-TAA   GTGCGCTTTCCGGCTTT   A   TTGCTTCGGGGCGGCGCTCGCTCCGGT           AGTCCTCATGCCGAGCGGCGCATCTGCCGGGCT           CGAAGCAA   T   AAAGCCGG   1923           CCGGCTTT   A   TTGCTTCG   1924               Waxy starch   CAGCTCGCCACCTCCGGCACCGTCCTCGGCATCACCGACAGGTT   1925       GBSS   CCGGCGTGCAGGTTTC   T   AGGGCGTGAGGCCCCGGAACCCGGCG         Triticum durum     GATGCGGCCCTCGTCATGAGGACTATCGGAGCGA       Gln28Term   TCGCTCCGATAGTCCTCATGACGAGGGCCGCATCCGCCGGGTTC   1926       CAG-TAG   CGGGGCCTCACGCCCT   A   GAAACCTGCACGCCGGAACCTGTCGGT           GATGCCGAGGACGGTGCCGGAGGTGGCGAGCTG           CAGGTTTC   T   AGGGCGTG   1927           CACGCCCT   A   GAAACCTG   1928               Waxy starch   CCCCGGAACCCGGCGGATGCGGCCCTCGTCATGAGGACTATCGG   1929       GBSS   AGCGAGCGCCGCCCCG   T   AGCAAAGCCGGAAAGCGCACCGCGGG         Triticum durum     AGCCGGCGGTGCCTCTCCATGGTGGTGCGCGCCA       Lys52Term   TGGCGCGCACCACCATGGAGAGGCACCGCCGGCTCCCGCGGTG   1930       AAG-TAG   CGCTTTCCGGCTTTGCT   A   CGGGGCGGCGCTCGCTCCGATAGTCCT           CATGACGAGGGCCGCATCCGCCGGGTTCCGGGG           CCGCCCCG   T   AGCAAAGC   1931           GCTTTGCT   A   CGGGGCGG   1932               Waxy starch   CGGAACCCGGCGGATGCGGCCCTCGTCATGAGGACTATCGGAGC   1933       GBSS   GAGCGCCGCCCCGAAG   T   AAAGCCGGAAAGCGCACCGCGGGAGC         Triticum durum CGGCGGTGCCTCTCCATGGTGGTGCGCGCCACGG         Gln53Term   CCGTGGCGCGCACCACCATGGAGAGGCACCGCCGGCTCCCGCG   1934       CAA-TAA   GTGCGCTTTCCGGCTTT   A   CTTCGGGGCGGCGCTCGCTCCGATAGT           CCTCATGACGAGGGCCGCATCCGCCGGGTTCCG           CCCCGAAG   T   AAAGCCGG   1935           CCGGCTTT   A   CTTCGGGG   1936               Waxy starch   GCGGATGCGGCCCTCGTCATGAGGACTATCGGAGCGAGCGCCGC   1937       GBSS   CCCGAAGCAAAGCCGG   T   AAGCGCACCGCGGGAGCCGGCGGTGC         Triticum durum     CTCTCCATGGTGGTGCGCGCCACGGGCAGCGGCG       Lys56Term   CGCCGCTGCCCGTGGCGCGCACCACCATGGAGAGGCACCGCCG   1938       AAA-TAA   GCTCCCGCGGTGCGCTT   A   CCGGCTTTGCTTCGGGGCGGGGCTCG           CTCCGATAGTCCTCATGACGAGGGCCGCATCCGC           AAAGCCGG   T   AAGCGCAC   1939           GTGCGCTT   A   CCGGCTTT   1940               Waxy starch   TATCGGAGCGAGCGCCGCCCCGAAGCAAAGCCGGAAAGCGCACC   1941       GBSS   GCGGGAGCCGGCGGTG   A   CTCTCCATGGTGGTGCGCGCCACGGG         Triticum durum     CAGCGGCGGCATGAACCTCGTGTTCGTCGGCGCC       Cys64Term   GGCGCCGACGAACACGAGGTTCATGCCGCCGCTGCCCGTGGCGC   1942       TGC-TGA   GCACCACCATGGAGAG   T   CACCGCCGGCTCCCGCGGTGCGCTTTC           CGGCTTTGCTTCGGGGCGGCGCTCGCTCCGATA           CGGCGGTG   A   CTCTCCAT   1943           ATGGAGAG   T   CACCGCCG   1944               Waxy starch   CAGCTCGCCACCTCCGGCACCGTCCTCGGCATCACCGACAGGTT   1945       GBSS   CCGGCGTGCAGGTTTT   T   AGGGTGTGAGGCCCCGGAGCCCGGCAG         Triticum turgidum     ATGCGCCGCTCGGCATGAGGACTACCGGAGCGA       Gln28Term   TCGCTCCGGTAGTCCTCATGCCGAGCGGCGCATCTGCGGGGCTC   1946       CAG-TAG   CGGGGCCTCACACCCT   A   AAAACGTGCACGCCGGAACCTGTCGGT           GATGCCGAGGACGGTGCCGGAGGTGGCGAGCTG           CAGGTTTT   T   AGGGTGTG   1947           CACACCCT   A   AAAACCTG   1948               Waxy starch   CCCCGGAGCCCGGCAGATGCGCCGCTCGGCATGAGGACTACCGG   1949       GBSS   AGCGAGCGCCGCCCCG   T   AGCAACAAAGCCGGAAAGCGCACCGCG         Triticum turgidum     GGACCCGGCGGTGCCTCTCCATGGTGGTGCGCG       Lys52Term   CGCGCACCACCATGGAGAGGCACCGCCGGGTCCCGCGGTGCGC   1950       AAG-TAG   TTTCCGGCTTTGTTGCT   A   CGGGGCGGCGCTCGCTCCGGTAGTCCT           CATGCCGAGCGGCGCATCTGCCGGGCTCCGGGG           CCGCCCCG   T   AGCAACAA   1951           TTGTTGCT   A   CGGGGCGG   1952               Waxy starch   CGGAGCCCGGCAGATGCGCCGCTCGGCATGAGGACTACCGGAG   1953       GBSS   CGAGCGCCGCCCCGAAG   T   AACAAAGCCGGAAAGCGCACCGCGG         Triticum turgidum     GACCCGGCGGTGCCTCTCCATGGTGGTGCGCGCCA       Gln53Term   TGGCGCGCACCACCATGGAGAGGCACCGCCGGGTCCCGCGGTG   1954       CAA-TAA   CGCTTTCCGGCTTTGTT   A   CTTCGGGGCGGCGCTCGCTCCGGTAGT           CCTCATGCCGAGCGGCGCATCTGCCGGGCTCCG           CCCCGAAG   T   AACAAAGC   1955           GCTTTGTT   A   CTTCGGGG   1956               Waxy starch   AGCCCGGCAGATGCGCCGCTCGGCATGAGGACTACCGGAGCGAG   1957       GBSS   CGCCGCCCCGAAGCAA   T   AAAGCCGGAAAGCGCACCGCGGGACCC         Triticum turgidum     GGCGGTGCCTCTCCATGGTGGTGCGCGCCACGG       Gln54Term   CCGTGGCGCGCACCACCATGGAGAGGCACCGCCGGGTCCCGCG   1958       CAA-TAA   GTGCGCTTTCCGGCTTT   A   TTGCTTCGGGGCGGCGCTCGCTCCGGT           AGTCCTCATGCCGAGCGGCGCATCTGCCGGGCT           CGAAGCAA   T   AAAGCCGG   1959           CCGGCTTT   A   TTGCTTCG   1960               Waxy starch   GATGCGCCGCTCGGCATGAGGACTACCGGAGCGAGCGCCGCCCC   1961       GBSS   GAAGCAACAAAGCCGG   T   AAGCGCACCGCGGGACCCGGCGGTGC         Triticum turgidum     CTCTCCATGGTGGTGCGCGCCACGGGCAGCGCCG       Lys57Term   CGGCGCTGCCCGTGGCGCGCACCACCATGGAGAGGCACCGCCG   1962       AAA-TAA   GGTCCCGCGGTGCGCTT   A   CCGGCTTTGTTGCTTCGGGGCGGCGC           TCGCTCCGGTAGTCCTCATGCCGAGCGGCGCATC           AAAGCCGG   T   AAGCGCAC   1963           GTGCGCTT   A   CCGGCTTT   1964               Waxy starch   CAGCTCGCCACCTCCGCCACCGTCCTCGGCATCACCGACAGGTTC   1965       GBSS   CGCCATGCAGGTTTC   T   AGGGCGTGAGGCCCCGGAGCCCGGCAGA         Aegilops speltoides     TGCGCCGCTCGGCATGAGGACTGTCGGAGCGA       Gln28Term   TCGCTCCGACAGTCCTCATGCCGAGCGGCGCATCTGCCGGGCTC   1966       CAG-TAG   CGGGGCCTCACGCCCT   A   GAAACCTGCATGGCGGAACCTGTCGGT           GATGCCGAGGACGGTGGCGGAGGTGGCGAGCTG           CAGGTTTC   T   AGGGCGTG   1967           CACGCCCT   A   GAAACCTG   1968               Waxy starch   GGTTTCCAGGGCGTGAGGCCCCGGAGCCCGGCAGATGCGCCGCT   1969       GBSS   CGGCATGAGGACTGTC   T   GAGCGAGCGCCGCCCCGAAGCAACAAA         Aegilops speltoides     GCCGGAAAGCGCACCGCGGGACCCGGCGGTGCC       Gly46Term   GGCACCGCCGGGTCCCGCGGTGCGCTTTCCGGCTTTGTTGCTTC   1970       GGA-TGA   GGGGCGGCGCTCGCTC   A   GACAGTCCTCATGCCGAGCGGCGCATC           TGCCGGGCTCCGGGGCCTCACGCCCTGGAAACC           GGACTGTC   T   GAGCGAGC   1971           GCTCGCTC   A   GACAGTCC   1972               Waxy starch   CCCCGGAGCCCGGCAGATGCGCCGCTCGGCATGAGGACTGTCGG   1973       GBSS   AGCGAGCGCCGCCCCG   T   AGCAACAAAGCCGGAAAGCGCACCGCG         Aegilops speltoides     GGACCCGGCGGTGCCTCTCGATGGTGGTGCGCG       Lys52Term   CGCGCACCACCATCGAGAGGCACCGCCGGGTCCCGCGGTGCGCT   1974       AAG-TAG   TTCCGGCTTTGTTGCT   A   CGGGGCGGCGCTCGCTCCGACAGTCCTC           ATGCCGAGCGGCGCATCTGCCGGGCTCCGGGG           CCGCCCCG   T   AGCAACAA   1975           TTGTTGCT   A   CGGGGCGG   1976               Waxy starch   CGGAGCCCGGCAGATGCGCCGCTCGGCATGAGGACTGTCGGAG   1977       GBSS   CGAGCGCCGCCCCGAAG   T   AACAAAGCCGGAAAGCGCACCGCGG         Aegilops speltoides     GACCCGGCGGTGCCTCTCGATGGTGGTGCGCGCCA       Gln53Term   TGGCGCGCACCACCATCGAGAGGCACCGCCGGGTCCCGCGGTG   1978       CAA-TAA   CGCTTTCCGGCTTTGTT   A   CTTCGGGGCGGCGCTCGCTCCGACAGT           CCTCATGCCGAGCGGCGCATCTGCCGGGCTCCG           CCCCGAAG   T   AACAAAGC   1979           GCTTTGTT   A   CTTCGGGG   1980               Waxy starch   AGCCCGGCAGATGCGCCGCTCGGCATGAGGACTGTCGGAGCGAG   1981       GBSS   CGCCGCCCCGAAGCAA   T   AAAGCCGGAAAGCGCACCGCGGGACCC         Aegilops speltoides     GGCGGTGCCTCTCGATGGTGGTGCGCGCCACCG       Gln54Term   CGGTGGCGCGCACCACCATCGAGAGGCACCGCCGGGTCCCGCG   1982       CAA-TAA   GTGCGCTTTCCGGCTTT   A   TTGCTTCGGGGCGGCGCTCGCTCCGAC           AGTCCTCATGCCGAGCGGCGCATCTGCCGGGCT           CGAAGCAA   T   AAAGCCGG   1983           CCGGCTTT   A   TTGCTTCG   1984               Waxy starch   AGTGCAGAGATCTTCCACAGCAACAGCTAGACAACCACCATGTCG   1985       GBSS   GCTCTCACCACGTCC   T   AGCTCGCCACCTCGGCCACCGGCTTCGG         Oryza glaberrima     CATCGCTGACAGGTCGGCGCCGTCGTCGCTGC       Gln8Term   GCAGCGACGACGGCGCCGACCTGTCAGCGATGCCGAAGCCGGT   1986       GAG-TAG   GGCCGAGGTGGCGAGCT   A   GGACGTGGTGAGAGCCGACATGGTG           GTTGTCTAGCTGTTGCTGTGGAAGATCTCTGCACT           CCACGTCC   T   AGCTCGCC   1987           GGCGAGCT   A   GGACGTGG   1988               Waxy starch   TCCACAGCAACAGCTAGACAACCACCATGTCGGCTCTCACCACGT   1989       GBSS   CCCAGCTCGCCACCT   A   GGCCACCGGCTTCGGCATCGCTGACAGG         Oryza glaberrima     TCGGCGCCGTCGTCGCTGCTCCGCCACGGGTT       Ser12Term   AACCCGTGGCGGAGCAGCGACGACGGCGCCGACCTGTCAGCGAT   1990       TCG-TAG   GCCGAAGCCGGTGGCC   T   AGGTGGCGAGCTGGGACGTGGTGAGA           GCCGACATGGTGGTTGTCTAGCTGTTGCTGTGGA           CGCCACCT   A   GGCCACCG   1991           CGGTGGCC   T   AGGTGGCG   1992               Waxy starch   CGGCTCTCACCACGTCCCAGCTCGCCACCTCGGCCACCGGCTTC   1993       GBSS   GGCATCGCTGACAGGT   A   GGCGCCGTCGTCGCTGCTCCGCCACGG         Oryza glaberrima     GTTCCAGGGCCTCAAGCCCCGCAGCCCCGCCGG       Ser22Term   CCGGCGGGGCTGCGGGGCTTGAGGCCCTGGAACCCGTGGCGGA   1994       TCG-TAG   GCAGCGACGACGGCGCC   T   ACCTGTCAGCGATGCCGAAGCCGGTG           GCCGAGGTGGCGAGCTGGGACGTGGTGAGAGCCG           TGACAGGT   A   GGCGCCGT   1995           ACGGCGCC   T   ACCTGTCA   1996               Waxy starch   CCACGTCCCAGCTCGCCACCTCGGCCACCGGCTTCGGCATCGCT   1997       GBSS   GACAGGTCGGCGCCGT   A   GTCGCTGCTCCGCCACGGGTTCCAGGG         Oryza glaberrima     CCTCAAGCCCCGCAGCCCCGCCGGCGGCGACGC       Ser25Term   GCGTCGCCGCCGGCGGGGCTGCGGGGCTTGAGGCCCTGGAACC   1998       TCG-TAG   CGTGGCGGAGCAGCGAC   T   ACGGCGCCGACCTGTCAGCGATGCCG           AAGCCGGTGGCCGAGGTGGCGAGCTGGGACGTGG           GGCGCCGT   A   GTCGCTGC   1999           GCAGCGAC   T   ACGGCGCC   2000               Waxy starch   CGTCCCAGCTCGCCACCTCGGCCACCGGCTTCGGCATCGCTGAC   2001       GBSS   AGGTCGGCGCCGTCGT   A   GCTGCTCCGCCACGGGTTCCAGGGCCT         Oryza glaberrima     CAAGCCCCGCAGCCCCGCCGGCGGCGACGCGAC       Ser26Term   GTCGCGTCGCCGCCGGCGGGGCTGCGGGGCTTGAGGCCCTGGA   2002       TCG-TAG   ACCCGTGGCGGAGCAGC   T   ACGACGGCGCCGACCTGTCAGCGATG           CCGAAGCCGGTGGCCGAGGTGGCGAGCTGGGACG           GCCGTCGT   A   GCTGCTCC   2003           GGAGCAGC   T   ACGACGGC   2004               Waxy starch   TCCACAGCAAGAGCTAAACAGCCGACCGTGTGCACCACCATGTCG   2005       GBSS   GCTGTCACCACGTCC   T   AGCTCGCCACCTCGGCCACCGGCTTCGG         Oryza sativa     CATCGCCGACAGGTCGGCGCCGTCGTCGCTGG       Gln8Term   GCAGCGACGACGGCGCCGACCTGTCGGCGATGCCGAAGCCGGT   2006       CAG-TAG   GGCCGAGGTGGCGAGCT   A   GGACGTGGTGAGAGCCGACATGGTG           GTGCACACGGTCGGCTGTTTAGCTCTTGCTGTGGA           CCACGTCC   T   AGCTCGCC   2007           GGCGAGCT   A   GGACGTGG   2008               Waxy starch   CTAAACAGCCGACCGTGTGCACCACCATGTCGGCTCTCACCACGT   2009       GBSS   CCCAGCTCGCCACCT   A   GGCCACCGGCTTCGGCATCGCCGACAGG         Oryza sativa     TCGGCGCCGTCGTCGCTGCTTCGCCACGGGTT       Ser12Term   AACCCGTGGCGAAGCAGCGACGACGGCGCCGACCTGTCGGCGAT   2010       TCG-TAG   GCCGAAGCCGGTGGCC   T   AGGTGGCGAGCTGGGACGTGGTGAGA           GCCGACATGGTGGTGCACACGGTCGGCTGTTTAG           CGCCACCT   A   GGCCACCG   2011           CGGTGGCC   T   AGGTGGCG   2012               Waxy starch   CGGCTCTCACCACGTCCCAGCTCGCCACCTCGGCCACCGGCTTC   2013       GBSS   GGCATCGCCGACAGGT   A   GGCGCCGTCGTCGCTGCTTCGCCACGG         Oryza sativa     GTTCCAGGGCCTCAAGCCCCGTAGCCCAGCCGG       Ser22Term   CCGGCTGGGCTACGGGGCTTGAGGCCCTGGAACCCGTGGCGAA   2014       TCG-TAG   GGAGCGACGACGGCGCC   T   ACCTGTCGGCGATGCCGAAGCCGGTG           GCCGAGGTGGCGAGCTGGGACGTGGTGAGAGCCG           CGACAGGT   A   GGCGCCGT   2015           ACGGCGCC   T   ACCTGTCG   2016               Waxy starch   CCACGTCCCAGCTCGCCACCTCGGCCACCGGCTTCGGCATCGCC   2017       GBSS   GACAGGTCGGCGCCGT   A   GTCGCTGCTTCGCCACGGGTTCCAGGG         Oryza sativa     CCTCAAGCCCCGTAGCCCAGCCGGCGGGGACGC       Ser25Term   GCGTCCCCGCCGGCTGGGCTACGGGGCTTGAGGCCCTGGAACCC   2018       TCG-TAG   GTGGCGAAGCAGCGAC   T   ACGGCGCCGACCTGTCGGCGATGCCGA           AGCCGGTGGCCGAGGTGGCGAGCTGGGACGTGG           GGCGCCGT   A   GTCGCTGC   2019           GCAGCGAC   T   ACGGCGCC   2020               Waxy starch   CGTCCCAGCTCGCCACCTCGGCCACCGGCTTCGGCATCGCCGAC   2021       GBSS   AGGTCGGCGCCGTCGT   A   GCTGCTTCGCCACGGGTTCCAGGGCCT         Oryza sativa     CAAGCCCCGTAGCCCAGCCGGCGGGGACGCATC       Ser26Term   GATGCGTCCCCGCCGGCTGGGCTACGGGGCTTGAGGCCCTGGAA   2022       TCG-TAG   CCCGTGGCGAAGCAGC   T   ACGACGGCGCCGACCTGTCGGCGATGC           CGAAGCCGGTGGCCGAGGTGGCGAGCTGGGACG           GCCGTCGT   A   GCTGCTTC   2023           GAAGCAGC   T   ACGACGGC   2024               Waxy starch   GTCTCTCACTGCAGGTAGCCACACCCTGTGCGCGGCGCCATGGC   2025       GBSS   GGCTCTGGCCACGTCC   T   AGCTCGCCACCTCCGGCACCGTCCTCG         Hordeum vulgare     GCGTCACCGACAGATTCCGGCGTCCAGGTTTTC       Gln8Term   GAAAACCTGGACGCCGGAATCTGTCGGTGACGCCGAGGACGGTG   2026       GAG-TAG   CCGGAGGTGGCGAGCT   A   GGACGTGGCCAGAGCCGGCATGGCGC           CGCGCACAGGGTGTGGCTACCTGCAGTGAGAGAC           CCACGTCC   T   AGCTCGCC   2027           GGCGAGCT   A   GGACGTGG   2028               Waxy starch   ATGGCGGCTCTGGCCACGTCCCAGCTCGCCACGTCCGGCACCGT   2029       GBSS   CCTCGGCGTCACCGAC   T   GATTCCGGCGTCCAGGTTTTGAGGGCCT         Hordeum vulgare     CAGGCCCCGGAACCCGGCGGATGCGGCGCTTG       Arg21Term   CAAGCGCGGCATCCGCCGGGTTCCGGGGCCTGAGGCCGTGAAAA   2030       AGA-TGA   CCTGGACGCCGGAATC   A   GTCGGTGACGCCGAGGACGGTGCCGG           AGGTGGCGAGCTGGGACGTGGCCAGAGCCGCCAT           TCACCGAC   T   GATTCCGG   2031           CCGGAATC   A   GTCGGTGA   2032               Waxy starch   CAGCTCGCCACCTCCGGCACCGTCCTCGGCGTCACCGACAGATT   2033       GBSS   CCGGCGTCCAGGTTTT   T   AGGGCCTCAGGCCCCGGAACCCGGCGG         Hordeum vulgare     ATGCGGCGCTTGGTCTGAGGACTATCGGAGCAA       Gln28Term   TTGCTCCGATAGTCCTCATACCAAGCGCCGCATCCGCCGGGTTCC   2034       CAG-TAG   GGGGCCTGAGGCCCT   A   AAAACCTGGACGCCGGAATCTGTCGGTG           ACGCCGAGGACGGTGCCGGAGGTGGCGAGCTG           CAGGTTTT   T   AGGGCCTC   2035           GAGGCCCT   A   AAAACCTG   2036               Waxy starch   GGTTTTCAGGGCCTCAGGCCGCGGAACCCGGCGGATGCGGCGCT   2037       GBSS   TGGTATGAGGACTATCTGAGCAAGCGCCGCCCCGAAGCAAAGGC         Hordeum vulgare     GGAAAGCGGACCGCGGGAGCCGGCGGTGCCTCT       Gly46Term   AGAGGCACCGCCGGCTCCCGCGGTGCGCTTTCCGGCTTTGCTTC   2038       GGA-TGA   GGGGCGGCGCTTGCTC   A   GATAGTCCTCATACCAAGCGCCGCATC           CGCCGGGTTCCGGGGCCTGAGGCCCTGAAAACC           GGACTATC   T   GAGCAAGC   2039           GCTTGCTC   A   GATAGTCC   2040               Waxy starch   CCCCGGAACCCGGCGGATGCGGCGCTTGGTATGAGGACTATCGG   2041       GBSS   AGCAAGCGCCGCCCCG   T   AGCAAAGCCGGAAAGCGCACCGCGGG         Hordeum vulgare     AGCCGGCGGTGCCTCTCCGTGGTGGTGAGCGCCA       Lys52Term   TGGCGCTCACCACCACGGAGAGGCACCGCCGGCTCCCGCGGTGC   2042       AAG-TAG   GCTTTGCGGCTTTGCT   A   CGGGGCGGCGCTTGCTCCGATAGTCCTC           ATACCAAGCGCCGCATCCGCCGGGTTCCGGGG           CCGCCCCG   T   AGCAAAGC   2043           GCTTTGCT   A   CGGGGCGG   2044               Waxy starch   ACGTCTTTTCTCTCTCTCCTACGCAGTGGATTAATCGGCATGGCGG   2045       GBSS   CTCTGGCCACGTCG   T   AGCTCGTCGCAACGCGGGCCGGCCTGGGC         Zea mays     GTCCCGGACGCGTCCACGTTCCGCCGCGGCG       Gln8Term   CGCCGCGGCGGAACGTGGACGCGTCCGGGACGCCCAGGCCGGC   2046       GAG-TAG   GCGCGTTGCGACGAGCT   A   CGACGTGGCCAGAGCCGCCATGCCGA           TTAATCCACTGCGTAGGAGAGAGAGAAAAGACGT           CCACGTCG   T   AGCTCGTC   2047           GACGAGCT   A   CGACGTGG   2048               Waxy starch   GTCGCAACGCGCGCCGGCCTGGGCGTCCCGGACGCGTCCACGTT   2049       GBSS   CCGCCGCGGCGCCGCG   T   AGGGCCTGAGGGGGGCCCGGGCGTCG         Zea mays     GCGGGGGCGGACACGCTCAGCATGCGGACCAGCG       Gln30Term   CGCTGGTCCGCATGCTGAGCGTGTCCGCCGCCGCCGACGCCCGG   2050       CAG-TAG   GCCCCCCTCAGGCCCT   A   CGCGGCGCCGCGGCGGAACGTGGACG           CGTCCGGGACGCCCAGGCCGGCGCGCGTTGCGAC           GCGCCGCG   T   AGGGCCTG   2051           CAGGCCCT   A   CGCGGCGC   2052               Waxy starch   TCCCGGACGCGTCCACGTTCCGCCGCGGCGCCGCGCAGGGCCT   2053       GBSS   GAGGGGGGCCCGGGCGT   A   GGCGGCGGCGGACACGCTCAGCATG         Zea mays     CGGACCAGCGCGCGCGCGGCGCCCAGGCACCAGCA       Ser38Term   TGCTGGTGCCTGGGCGCCGCGCGCGCGCTGGTCCGCATGCTGAG   2054       TCG-TAG   CGTGTCCGCCGCCGCC   T   ACGCCCGGGCCCCCCTCAGGCCCTGCG           CGGCGCCGCGGCGGAACGTGGACGCGTCCGGGA           CCGGGCGT   A   GGCGGCGG   2055           CCGCCGCC   T   ACGCCCGG   2056               Waxy starch   GCGTCGGCGGCGGCGGACACGCTCAGCATGCGGACCAGCGCGC   2057       GBSS   GCGCGGCGCCCAGGCAC   T   AGCAGCAGGCGCGCCGCGGGGGCAG         Zea mays     GTTCCCGTCGCTCGTCGTGTGCGCCAGCGCCGGCA       Ser57Term   TGCCGGCGCTGGCGCACACGACGAGCGACGGGAACCTGCCCCC   2058       GAG-TAG   GCGGCGCGCCTGCTGCT   A   GTGCCTGGGCGCCGCGCGCGCGCTG           GTCCGCATGCTGAGCGTGTCCGCCGCCGCCGACGC           CCAGGCAC   T   AGCAGCAG   2059           CTGCTGCT   A   GTGCCTGG   2060               Waxy starch   TCGGCGGCGGCGGACACGCTCAGCATGCGGACCAGCGCGCGCG   2061       GBSS   CGGCGCCCAGGCACCAG   T   AGCAGGCGCGCCGCGGGGGCAGGTT         Zea mays     CCCGTCGCTCGTCGTGTGCGCCAGCGCCGGCATGA       Gln58Term   TCATGCCGGGGCTGGCGCACACGACGAGCGACGGGAACCTGCCC   2062       CAG-TAG   CCGCGGCGCGCCTGCT   A   CTGGTGCCTGGGCGCCGCGCGCGCGC           TGGTCCGCATGCTGAGCGTGTCCGCCGCCGCCGA           GGCACCAG   T   AGCAGGCG   2063           CGCCTGCT   A   CTGGTGCC   2064                    
     EXAMPLE 11  
     Altering Fatty Acid Content of Plants  
     [0143] Improved means to manipulate fatty acid compositions, from biosynthetic or natural plant sources, are needed. For example, oils containing reduced saturated fatty acids are desired for dietary reasons and oils containing increased saturated fatty acids are also needed as alternatives to current sources of highly saturated oil products, such as tropical oils or chemically hydrogenated oils. It would therefore be advantageous to influence directly the production and composition of fatty acids in crop plants.  
     [0144] Higher plants synthesize fatty acids, primarily palmitic, stearic and oleic acids, in the plastids (i.e., chloroplasts, proplastids, or other related organelles) as part of the Fatty Acid Synthase (FAS) complex. Fatty acid synthesis is the result of the three enzymatic activities: acyl-ACP elongase, acyl-ACP desaturase and acyl-ACP thioesterases specific for each of palmitoyl-, stearoyl- and oleoyl-ACP.  
     [0145] A variety of enzymes have been identified that influence the relative levels of saturated vs. unsaturated fatty acids in plants. For example, the enzymes stearoyl-acyl carrier protein (stearoyl-ACP) desaturase, oleoyl desaturase and linoleate desaturase produce unsaturated fatty acids from saturated precursors. Similarly, relative enzymatic activities of the various acyl-ACP thioesterases influences the relative acyl-chain composition of the resultant fatty acids. Consequently a reduction or an increase of the activity of these enzymes can alter the properties of oils produced in a plant. In fact, specific targeting of particular enzymatic activities can results in altered levels of particular fatty acids.  
     [0146] The attached tables disclose exemplary oligonucleotides base sequences which can be used to generate site-specific mutations in plant genes encoding proteins involved in fatty acid biosynthesis.  
                   TABLE 22                          Oligonucleotides to produce plants with reduced palmitate                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               Reduced palmitate   TTTGGTGGCAGTGTCTTTGAACGCTTCATCTCCTCGTCATGGTGGC   2065           Acyl-ACP-thioesterase   CACCTCTGCTACGT   A   GTCATTCTTTCCTGTACCATCTTCTTCACTTG         Arabidopsis thaliana     ATCCTAATGGAAAAGGCAATAAGATTGG       Ser8Term   CCAATCTTATTGCCTTTTCCATTAGGATCAAGTGAAGAAGATGGTA   2066       TCG-TAG   CAGGAAAGAATGAC   T   ACGTCGCAGAGGTGGCCACCATGACGAGG           AGATGAAGCGTTCAAAGACACTGCCACCAAA           TGCTACGT   A   GTCATTCT   2067           AGAATGAC   T   ACGTAGCA   2068               Reduced palmitate   GGTGGCAGTGTCTTTGAACGCTTCATCTCCTCGTCATGGTGGCCA   2069       Acyl-ACP-thioesterase   CCTCTGCTACGTCGT   G   ATTCTTTCCTGTACCATCTTCTTCACTTGAT         Arabidopsis thaliana     CCTAATGGAAAAGGCAATAAGATTGGGTC       Ser9Term   GACCCAATCTTATTGCCTTTTCCATTAGGATCAAGTGAAGAAGATG   2070       TCA-TGA   GTACAGGAAAGAAT   C   ACGACGTAGCAGAGGTGGCCACCATGACG           AGGAGATGAAGCGTTCAAAGACACTGCCACC           TACGTCGT   G   ATTCTTTC   2071           GAAAGAAT   C   ACGACGTA   2072               Reduced palmitate   ATCTCCTCGTCATGGTGGCCACCTCTGCTACGTCGTCATTCTTTCC   2073       Acyl-ACP-thioesterase   TGTACCATCTTCTT   G   ACTTGATCCTAATGGAAAAGGCAATAAGATT         Arabidopsis thaliana     GGGTCTACGAATCTTGCTGGACTCAATTC       Ser17Term   GAATTGAGTCCAGCAAGATTCGTCGACCCAATCTTATTGCCTTTTC   2074       TCA-TGA   CATTAGGATCAAGT   C   AAGAAGATGGTCCAGGAAAGAATGACGACG           TAGCAGAGGTGGCCACCATGACGAGGAGAT           ATCTTCTT   G   ACTTGATC   2075           GATCAAGT   C   AAGAAGAT   2076               Reduced palmitate   GTGGCCACCTCTGCTACGTCGTCATTCTTTCCTGTACCATCTTCTT   2077       Acyl-AGP-thioesterase   CACTTGATCCTAAT   T   GAAAAGGCAATAAGATTGGGTCTACGAATCT         Arabidopsis thaliana     TGCTGGACTCAATTCTGCACCTAACTCTG       Gly22Term   CAGAGTTAGGTGCAGAATTGAGTCCAGCAAGATTCGTCGACCCAA   2078       GGA-TGA   TCTTATTGCCTTTTC   A   ATTAGGATCAAGTGAAGAAGATGGTCCAGG           AAAGAATGACGACGTAGCAGAGGTGGCCAC           ATCCTAAT   T   GAAAAGGC   2079           GCCTTTTC   A   ATTAGGAT   2080               Reduced palmitate   GCTTGAATTTGTGATCTGATTGGTTAATTGTGGCCACAATGGTTGC   2081       Acyl-ACP-thioesterase   TACTGCCGCCACGT   G   ATCATTCTTTCCGTTGACTTCCCCTTCTGGG         Garcinia mangostana     GATGCCAAATCGGGCAATCCCGGAAAAGG       Ser8Term   CCTTTTCCGGGATTGCCCGATTTGGCATCCCCAGAAGGGGAAGTC   2082       TCA-TGA   AACGGAAAGAATGAT   C   ACGTGGCGGCAGTAGCAACCATTGTGGCC           ACAATTAACCAATCAGATCACAAATTCAAGC           CGCCACGT   G   ATCATTCT   2083           AGAATGAT   C   ACGTGGCG   2084               Reduced palmitate   TGAATTTGTGATCTGATTGGTTAATTGTGGCCACAATGGTTGCTAC   2085       Acyl-ACP-thioesterase   TGCCGCCACGTCAT   G   ATTCTTTCCGTTGACTTCCCCTTCTGGGGAT         Garcinia mangostana     GCCAAATCGGGCAATCCCGGAAAAGGGTC       Ser9Term   GACCCTTTTCCGGGATTGCCCGATTTGGCATCCCCAGAAGGGGAA   2086       TCA-TGA   GTCAACGGAAAGAAT   C   ATGACGTGGCGGCAGTAGCAACCATTGTG           GCCACAATTAACCAATCAGATCACAAATTCA           CACGTCAT   G   ATTCTTTC   2087           GAAAGAAT   C   ATGACGTG   2088               Reduced palmitate   CTGATTGGTTAATTGTGGCCACAATGGTTGCTACTGCCGCCACGT   2089       Acyl-ACP-thioesterase   CATCATTCTTTCCGT   A   GACTTCCCCTTCTGGGGATGCCAAATCGGG         Garcinia mangostana     CAATCCCGGAAAAGGGTCGGTGAGTTTTGG       Leu13Term   CCAAAACTCACCGACCCTTTTCCGGGATTGCCCGATTTGGCATCC   2090       TTG-TAG   CCAGAAGGGGAAGTC   T   ACGGAAAGAATGATGACGTGGCGGCAGT           AGCAACCATTGTGGCCACAATTAACCAATCAG           CTTTCCGT   A   GACTTCCC   2091           GGGAAGTC   T   ACGGAAAG   2092               Reduced palmitate   ATGGTTGCTACTGCCGCCACGTCATCATTCTTTCCGTTGACTTCCC   2093       Acyl-ACP-thioesterase   CTTCTGGGGATGCC   T   AATCGGGCAATCCCGGAAAAGGGTCGGTG         Garcinia mangostana     AGTTTTGGGTCAATGAAGTCGAAATCCGCGG       Lys21Term   CCGCGGATTTCGACTTCATTGACCCAAAACTCACCGACCCTTTTCC   2094       AAA-TAA   GGGATTGCCCGATT   A   GGCATCCCCAGAAGGGGAAGTCAACGGAA           AGAATGATGACGTGGCGGCAGTCGCAACCAT           GGGATGCC   T   AATCGGGC   2095           GCCCGATT   A   GGCATCCC   2096               Reduced palmitate   GGGATTTCAGCACGAAATTGAAGTTGTTTTTAAAAACCATGGTTGC   2097       Acyl-ACP-thioesterase   TACTGCTGTGACAT   A   GGCGTTTTTCCCAGTCACTTCTTCACCTGAC         Gossypium hirsutum     TCCTCTGACTCGAAAAACAAGAAGCTCGG       Ser8Term   CCGAGCTTCTTGTTTTTCGAGTCAGAGGAGTCAGGTGAAGAAGTG   2098       TCG-TAG   ACTGGGAAAAACGCC   T   ATGTCACAGCAGTAGCAACCATGGTTTTTA           AAAACAACTTCAATTTCGTGCTGAAATCCC           TGTGACAT   A   GGCGTTTT   2099           AAAACGCC   T   ATGTCACA   2100               Reduced palmitate   TGTTTTTAAAAACCATGGTTGCTACTGCTGTGACATCGGCGTTTTT   2101       Acyl-ACP-thioesterase   CCCAGTCACTTCTT   G   ACCTGACTCCTCTGACTCGAAAAACAAGAAG         Gossypium hirsutum     CTCGGAAGCATCAAGTCGAAGCCATCGGT       Ser16Term   ACCGATGGCTTCGACTTGATGCTTCCGAGCTTCTTGTTTTTCGAGT   2102       TCA-TGA   CAGAGGAGTCAGGT   C   AAGAAGTGACTGGGAAAAACGCCGATGTCA           CAGCAGTAGCAACCATGGTTTTTAAAAACA           CACTTCTT   G   ACCTGACT   2103           AGTCAGGT   C   AAGAAGTG   2104               Reduced palmitate   TTGCTACTGCTGTGACATCGGCGTTTTTCCCAGTCACTTCTTCACC   2105       Acyl-ACP-thioesterase   TGACTCCTCTGACT   A   GAAAAACAAGAAGCTCGGAAGCATCAAGTC         Gossypium hirsutum     GAAGCCATCGGTTTCTTCTGGAAGTTTGCA       Ser22Term   TGCAAACTTCCAGAAGAAACCGATGGCTTCGACTTGATGCTTCCG   2106       TCG-TAG   AGCTTCTTGTTTTTC   T   AGTCAGAGGAGTCAGGTGAAGAAGTGACTG           GGAAAAACGCCGATGTCACAGCAGTCGCAA           CTCTGACT   A   GAAAAACA   2107           TGTTTTTC   T   AGTCAGAG   2108               Reduced palmitate   GCTACTGCTGTGACATCGGCGTTTTTCCCAGTCACTTCTTCACCTG   2109       Acyl-ACP-thioesterase   ACTCCTCTGACTCG   T   AAAACAAGAAGCTCGGAAGCATCAAGTCGA         Gossypium hirsutum     AGCCATCGGTTTGTTCTGGAAGTTTGCAAG       Lys23Term   CTTGCAAACTTCCAGAAGAAACCGATGGCTTCGACTTGATGCTTCC   2110       AAA-TAA   GAGCTTCTTGTTTT   A   CGAGTCAGAGGAGTCAGGTGAAGAAGTGAC           TGGGAAAAACGCCGATGTCACAGCAGTAGC           CTGACTCG   T   AAAACAAG   2111           CTTGTTTT   A   GGAGTCAG   2112               Reduced palmitate   CTCCCGCTCGTTGAAAGACAATGGTGGCTACCGCTGCAAGCTCTG   2113       Acyl-ACP-thioesterase   CATTCTTCCCCGTGT   A   GTCCCCGGTCACCTCCTCTAGACCAGGAA         Cuphea hookeriana     AGCCCGGAAATGGGTCATCGAGCTTCAGCCC       Ser14Term   GGGCTGAAGCTCGATGACCCATTTCCGGGCTTTCCTGGTCTAGAG   2114       TCG-TAG   GAGGTGACCGGGGAC   T   ACACGGGGAAGAATGCAGAGCTTGCAGC           GGTAGCCACCATTGTCTTTCAACGAGCGGGAG           CCCCGTGT   A   GTCCCCGG   2115           CCGGGGAC   T   ACACGGGG   2116               Reduced palmitate   ATGGTGGCTACCGCTGCAAGCTCTGCATTCTTCCCCGTGTCGTCC   2117       Acyl-ACP-thioesterase   CCGGTCACCTCCTCT   T   GACCAGGAAAGCCCGGAAATGGGTCATCG         Cuphea hookeriana     AGCTTCAGCCCCATCAAGCCCAAATTTGTCG       Arg21Term   CGACAAATTTGGGCTTGATGGGGCTGAAGCTCGATGACCCATTTC   2118       AGA-TGA   CGGGCTTTCCTGGTC   A   AGAGGAGGTGACCGGGGACGACACGGG           GAAGAATGCAGAGCTTGCAGCGGTAGCCACCAT           CCTCCTCT   T   GACCAGGA   2119           TCCTGGTC   A   AGAGGAGG   2120               Reduced palmitate   GCTACCGCTGCAAGCTCTGCATTCTTCCCCGTGTCGTCCCCGGTC   2121       Acyl-ACP-thioesterase   ACCTCCTCTAGACCA   T   GAAAGCCCGGAAATGGGTCATCGAGCTTC         Cuphea hookeriana     AGCCCCATCAAGCCCAAATTTGTCGCCAATG       Gly23Term   CATTGGCGACAAATTTGGGCTTGATGGGGCTGAAGCTCGATGACC   2122       GGA-TGA   CATTTCCGGGCTTTC   A   TGGTCTAGAGGAGGTGACCGGGGACGAC           ACGGGGAAGAATGCAGAGCTTGCAGCGGTAGC           CTAGACCA   T   GAAAGCCC   2123           GGGCTTTC   A   TGGTCTAG   2124               Reduced palmitate   ACCGCTGCAAGCTCTGCATTCTTCCCCGTGTCGTCCCCGGTCACC   2125       Acyl-ACP-thioesterase   TCCTCTAGACCAGGA   T   AGCCCGGAAATGGGTCATGGAGCTTCAGC         Cuphea hookeriana     CCCATCAAGCCCAAATTTGTCGCCAATGGCG       Lys24Term   CGCCATTGGCGACAAATTTGGGCTTGATGGGGCTGAAGCTCGATG   2126       AAG-TAG   ACCCATTTCCGGGCT   A   TCCTGGTCTAGAGGAGGTGACCGGGGAC           GACACGGGGAAGAATGCAGAGCTTGCAGCGGT           GACCAGGA   T   AGCCCGGA   2127           TCCGGGCT   A   TCCTGGTC   2128               Reduced palmitate   GCCACCGCTGCAAGTTCTGCATTCTTCCCCCTGCCGTCCCCGGAC   2129       Acyl-ACP-thioesterase   ACCTCCTCTAGGCCG   T   GAAAGCTGGGAAATGGGTCATCGAGCTTG         Cuphea lanceolata     AGCCCCCTCAAGCCCAAATTTGTCGCCAATG       Gly23Term   CATTGGCGACAAATTTGGGCTTGAGGGGGCTCAAGCTCGATGACC   2130       GGA-TGA   CATTTCCGAGCTTTC   A   CGGCCTAGAGGAGGTGTCCGGGGACGGC           AGGGGGAAGAATGCAGAACTTGCAGCGGTGGC           CTAGGCCG   T   GAAAGCTC   2131           GAGCTTTC   A   CGGCCTAG   2132               Reduced palmitate   ACCGCTGCAAGTTCTGCATTCTTCCCCCTGCCGTCCCCGGACACC   2133       Acyl-ACP-thioesterase   TCCTCTAGGCCGGGA   T   AGCTCGGAAATGGGTCATCGAGCTTGAGC         Cuphea lanceolata     CCCCTCAAGCCCAAATTTGTCGCCAATGCCG       Lys24Term   CGGCATTGGCGACAAATTTGGGCTTGAGGGGGCTCAAGCTCGAT   2134       AAG-TAG   GACCCATTTCCGAGCT   A   TCCCGGCCTAGAGGAGGTGTCCGGGGA           CGGCAGGGGGAAGAATGCAGAACTTGCAGCGGT           GGCCGGGA   T   AGCTCGGA   2135           TCCGAGCT   A   TCCCGGCC   2136               Reduced palmitate   GCAAGTTCTGCATTCTTCCCCCTGCCGTCCCCGGACACCTCCTCT   2137       Acyl-ACP-thioesterase   AGGCCGGGAAAGCTC   T   GAAATGGGTCATCGAGCTTGAGCCCCCT         Cuphea lanceolata     CAAGCCCAAATTTGTCGCCAATGCCGGGTTGA       Gly26Term   TCAACCCGGCATTGGCGACAAATTTGGGGTTGAGGGGGCTCAAGC   2138       GGA-TGA   TCGATGACCCATTTC   A   GAGCTTTCCCGGCCTAGAGGAGGTGTCCG           GGGACGGCAGGGGGAAGAATGCAGAACTTGC           GAAAGCTC   T   GAAATGGG   2139           CCCATTTC   A   GAGCTTTC   2140               Reduced palmitate   CATTCTTCCCCCTGCCGTCCCCGGACACCTCCTCTAGGCCGGGAA   2141       Acyl-ACP-thioesterase   AGCTCGGAAATGGGT   G   ATCGAGCTTGAGCCCCCTCAAGCCCAAAT         Cuphea lanceolata     TTGTCGCCAATGCCGGGTTGAAGGTTAAGGC       Ser29Term   GCCTTAACCTTCAACCCGGCATTGGCGACAAATTTGGGCTTGAGG   2142       TCA-TGA   GGGCTCAAGCTCGAT   C   ACCCATTTCCGAGCTTTCCCGGCCTAGAG           GAGGTGTCCGGGGACGGCAGGGGGAAGAATG           AAATGGGT   G   ATCGAGCT   2143           AGCTCGAT   C   ACCCATTT   2144               Reduced palmitate   CGTTTAAGTGGATCGGACATTTAAGTGTTTTAATCATGGTAGCTAT   2145       Acyl-ACP-thioesterase   GAGTGCTACTGCGT   A   GCTGTTTCCGGTTTCTTCCCCAAAACCTCAC         Helianthus annuus     TCTGGAGCCAAGACATCTGATAAGCTTGG       Ser9Term   CCAAGCTTATCAGATGTCTTGGCTCCAGAGTGAGGTTTTGGGGAA   2146       TCG-TAG   GAAACCGGAAACAGC   T   ACGCAGTCGCACTCATAGCTACCATGATT           AAAACACTTAAATGTCCGATCCACTTAAACG           TACTGCGT   A   GCTGTTTC   2147           GAAACAGC   T   ACGCAGTA   2148               Reduced palmitate   AGTGTTTTAATCATGGTCGCTATGAGTGCTACTGCGTCGCTGTTTC   2149       Acyl-ACP-thioesterase   CGGTTTCTTCCCCA   T   AACCTCACTCTGGAGCCAAGACATCTGATAA         Helianthus annuus     GCTTGGAGGTGAACCAGGTAGTGTTGCTG       Lys17Term   CAGCAACACTACCTGGTTCACCTCCAAGCTTATCAGATGTCTTGGC   2150       AAA-TAA   TCCAGAGTGAGGTT   A   TGGGGAAGAAACCGGAAACAGCGACGCAG           TAGCACTCATAGCTACCATGATTAAAACACT           CTTCCCCA   T   AACCTCAC   2151           GTGAGGTT   A   TGGGGAAG   2152               Reduced palmitate   ATGGTAGCTATGAGTGCTACTGCGTCGCTGTTTCCGGTTTCTTCCC   2153       Acyl-ACP-thioesterase   CAAAACCTCACTCT   T   GAGCCAAGACATCTGATAAGCTTGGAGGTG         Helianthus annuus     AACCAGGTAGTGTTGCTGTGCGCGGAATCA       Gly21Term   TGATTCCGCGCACAGCAACACTACCTGGTTCACCTCCAAGCTTATC   2154       GGA-TGA   AGATGTCTTGGCTC   A   AGAGTGAGGTTTTGGGGAAGAAACCGGAAA           CAGCGACGCAGTAGCACTCATAGCTACCAT           CTCACTCT   T   GAGCCAAG   2155           CTTGGCTC   A   AGAGTGAG   2156               Reduced palmitate   GCTATGAGTGCTACTGCGTCGCTGTTTCCGGTTTCTTCCCCAAAAC   2157       Acyl-ACP-thioesterase   CTCACTCTGGAGCCT   A   GACATCTGATAAGCTTGGAGGTGAACCAG         Helianthus annuus     GTAGTGTTGCTGTGCGCGGAATCAAGACAA       Lys23Term   TTGTCTTGATTCCGCGCACAGCAACACTACCTGGTTCACCTCCAAG   2158       AAG-TAG   CTTATCAGATGTCT   A   GGCTCCAGAGTGAGGTTTTGGGGAAGAAAC           CGGAAACAGCGACGCAGTCGCACTCATAGC           CTGGAGCC   T   AGACATCT   2159           AGATGTCT   A   GGCTCCAG   2160               Reduced palmitate   ATGGTGGCTGCTGCAGCAAGTTCTGCATGCTTCCCTGTTCCATCC   2161       Acyl-ACP-thioesterase   CCAGGAGCCTCCCCT   T   AACCTGGGAAGTTAGGCAACTGGTCATCG         Cuphea palustris     AGTTTGAGCCCTTCCTTGAAGCCCAAGTCAA       Lys21Term   TTGACTTGGGCTTCAAGGAAGGGCTCAAACTCGATGACCAGTTGC   2162       AAA-TAA   CTAACTTCCCAGGTT   A   AGGGGAGGCTCCTGGGGATGGAACAGGG           AAGCATGCAGAACTTGCTGCAGCAGCCACCAT           CCTCCCCT   T   AACCTGGG   2163           CCCAGGTT   A   AGGGGAGG   2164               Reduced palmitate   GCTGCAGCAAGTTCTGCATGCTTCCCTGTTCCATCCCCAGGAGCC   2165       Acyl-ACP-thioesterase   TCCCCTAAACCTGGG   T   AGTTAGGCAACTGGTCATCGAGTTTGAGC         Cuphea palustris     CCTTCCTTGAAGCCCAAGTCAATCCCCAATG       Lys24Term   CATTGGGGATTGACTTGGGCTTCAAGGAAGGGCTCAAACTCGATG   2166       AAG-TAG   ACCAGTTGCCTAACT   A   CCCAGGTTTAGGGGAGGCTCCTGGGGATG           GAACAGGGAAGCATGCAGAACTTGCTGCAGC           AACCTGGG   T   AGTTAGGC   2167           GCCTAACT   A   CCCAGGTT   2168               Reduced palmitate   TGCATGCTTCCCTGTTCCATCCCCAGGAGCCTCCCCTAAACCTGG   2169       Acyl-ACP-thioesterase   GAAGTTAGGCAACTG   A   TCATCGAGTTTGAGCCCTTCCTTGAAGCC         Cuphea palustris     CAAGTCAATCCCCAATGGCGGATTTCAGGTT       Trp28Term   AACCTGAAATCCGCCATTGGGGATTGACTTGGGCTTCAAGGAAGG   2170       TGG-TGA   GCTCAAACTCGATGA   T   CAGTTGCCTAACTTCCCAGGTTTAGGGGA           GGCTCCTGGGGATGGAACAGGGAAGCATGCA           GGCAACTG   A   TCATCGAG   2171           CTCGATGA   T   CAGTTGCC   2172               Reduced palmitate   CATGCTTCCCTGTTCCATCCCCAGGAGCCTCCCCTAAACCTGGGA   2173       Acyl-ACP-thioesterase   AGTTAGGCAACTGGT   G   ATCGAGTTTGAGCCCTTCCTTGAAGCCCA         Cuphea palustris     AGTCAATCCCCAATGGCGGATTTCAGGTTAA       Ser29Term   TTAACCTGAAATCCGCCATTGGGGATTGACTTGGGCTTCAAGGAA   2174       TCA-TGA   GGGCTCAAACTCGAT   C   ACCAGTTGCCTAACTTCCCAGGTTTAGGG           GAGGCTCCTGGGGATGGAACAGGGAAGCATG           CAACTGGT   G   ATCGAGTT   2175           AACTCGAT   C   ACCAGTTG   2176               Reduced paimitate   ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACC   2175       Acyl-ACP-thioesterase   CCGGGAATCTCCCCT   T   AACCCGGGAAGTTCGGTAATGGTGGCTTT         Cuphea hookeriana     CAGGTTAAGGCAAACGCCAATGCCCATCCTA        Lys21Term   TAGGATGGGCATTGGCGTTTGCCTTAACCTGAAAGCCACCATTAC   2178       AAA-TAA   CGAACTTCCCGGGTT   A   AGGGGAGATTCCCGGGGTTGGAACGGAG           AAGAATGCAGAACTTGCTGCGGCAGCCACCAT           TCTCCCCT   T   AACCCGGG   2179           CCCGGGTT   A   AGGGGAGA   2180               Reduced palmitate   GCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCCCGGGAATC   2181       Acyl-ACP-thioesterase   TCCCCTAAACCCGGG   T   AGTTCGGTAATGGTGGCTTTCAGGTTAAG         Cuphea hookeriana     GCAAACGCCAATGCCCATCCTAGTCTAAAGT       Lys24Term   ACTTTAGACTAGGATGGGCATTGGCGTTTGCCTTAACCTGAAAGC   2182       AAG-TAG   CACCATTACCGAACT   A   CCCGGGTTTAGGGGAGATTCCCGGGGTTG           GAACGGAGAAGAATGCAGAACTTGCTGCGGC           AACCCGGG   T   AGTTCGGT   2183           ACCGAACT   A   CCCGGGTT   2184               Reduced palmitate   TTCTCCGTTCCAACCCCGGGAATCTCCCCTAAACCCGGGAAGTTC   2185       Acyl-ACP-thioesterase   GGTAATGGTGGCTTT   T   AGGTTAAGGCAAACGCCAATGCCCATCCT         Cuphea hookeriana     AGTCTAAAGTCTGGCAGCCTCGAGACTGAAG       Gln31Term   CTTCAGTCTCGAGGCTGCCAGACTTTAGACTAGGATGGGCATTGG   2186       CAG-TAG   CGTTTGCCTTAACCT   A   AAAGCCACCATTACCGAACTTCCCGGGTTT           AGGGGAGATTCCCGGGGTTGGAACGGAGAA           GTGGCTTT   T   AGGTTAAG   2187           CTTAACCT   A   AAAGCCAC   2188               Reduced palmitate   GTTCCAACCCCGGGAATCTCCCCTAAACCCGGGAAGTTCGGTAAT   2189       Acyl-ACP-thioesterase   GGTGGCTTTCAGGTT   T   AGGCAAACGCCAATGCCCATCCTAGTCTA         Cuphea hookeriana     AAGTCTGGCAGCCTCGAGACTGAAGATGACA       Lys33Term   TGTCATCTTCAGTCTCGAGGCTGCCAGACTTTAGACTAGGATGGG   2190       AAG-TAG   CATTGGCGTTTGCCT   A   AACCTGAAAGCCACCATTACCGAACTTCCC           GGGTTTAGGGGAGATTCCCGGGGTTGGAAC           TTCAGGTT   T   AGGCAAAC   2191           GTTTGCCT   A   AACCTGAA   2192               Reduced palmitate   ATGTTGAAGCTCTCGTGTAATGCGACTGATAAGTTACAGACCCTCT   2193       Acyl-ACP-thioesterase   TCTCGCATTCTCAT   T   AACCGGATCCGGCACACCGGAGAACCGTCT         Brassica rapa     CCTCCGTGTCGTGCTCTCATCTGAGGAAAC       Gln21Term   GTTTCCTCAGATGAGAGCACGACACGGAGGAGACGGTTCTCCGGT   2194       CAA-TAA   GTGCCGGATCCGGTT   A   ATGAGAATGCGAGAAGAGGGTCTGTAACT           TATCAGTCGCATTACACGAGAGCTTCAACAT           ATTCTCAT   T   AACCGGAT   2195           ATCCGGTT   A   ATGAGAAT   2196               Reduced palmitate   GCGACTGATAAGTTACAGACCCTCTTCTCGCATTCTCATCAACCGG   2197       Acyl-ACP-thioesterase   ATCCGGCACACCGG   T   GAACCGTCTCCTCCGTGTCGTGCTCTCATC         Brassica rapa     TGAGGAAACCGGTTCTCGATCCTTTGCGAG       Arg28Term   CTCGCAAAGGATCGAGAACCGGTTTCCTCAGATGAGAGCACGACA   2198       AGA-TGA   CGGAGGAGACGGTTC   A   CCGGTGTGCCGGATCCGGTTGATGAGAA           TGCGAGAAGAGGGTCTGTAACTTATCAGTCGC           CACACCGG   T   GAACCGTC   2199           GACGGTTC   A   CCGGTGTG   2200               Reduced palmitate   CCCTCTTCTCGCATTCTCATCAACCGGATCCGGCACACCGGAGAA   2201       Acyl-ACP-thioesterase   CCGTCTCCTCCGTGT   A   GTGCTCTCATCTGAGGAAACCGGTTCTCG         Brassica rapa     ATCCTTTGCGAGCGATCGTATCTGCTGATCA       Ser24Term   TGATCAGCAGATACGATCGCTCGCAAAGGATCGAGAACCGGTTTC   2202       TCG-TAG   CTCAGATGAGAGCAC   T   ACACGGAGGAGACGGTTCTCCGGTGTGC           CGGATCCGGTTGATGAGAATGCGAGAAGAGGG           CTCCGTGT   A   GTGCTCTC   2203           GAGAGCAC   T   ACACGGAG   2204               Reduced palmitate   CTTCTCGCATTCTCATCAACCGGATCCGGCACACCGGAGAACCGT   2205       Acyl-ACP-thioesterase   CTCCTCCGTGTCGTG   A   TCTCATCTGAGGAAACCGGTTCTCGATCC         Brassica rapa     TTTGCGAGCGATCGTATCTGCTGATCAAGGA       Cys25Term   TCCTTGATCAGCAGATACGATCGCTCGCAAAGGATCGAGAACCGG   2206       TGC-TGA   TTTCCTCAGATGAGA   T   CACGACACGGAGGAGACGGTTCTCCGGTG           TGCCGGATCCGGTTGATGAGAATGCGAGAAG           GTGTCGTG   A   TCTCATCT   2207           AGATGAGA   T   CACGACAC   2208               Reduced palmitate   ATTCTTCTTCTATAAACCAAAACCTCAGGAACCATAAAAAAAAAAGG   2209       Acyl-ACP-thioesterase   GCATCAAAAATGT   A   GAAGCTTTCGTGTAATGTGACTAACAACTTAC         Brassica napus     ACACCTTCTCCTTCTTCTCCGATTCCTC       Leu2Term   GAGGAATCGGAGAAGAAGGAGAAGGTGTGTAAGTTGTTAGTCACA   2210       TTG-TAG   TTACACGAAAGCTTC   T   ACATTTTTGATGCCCTTTTTTTTTTATGGTTC           CTGAGGTTTTGGTTTATAGAAGAAGAAT           AAAAATGT   A   GAAGCTTT   2211           AAAGCTTC   T   ACATTTTT   2212               Reduced palmitate   TCTTCTTCTATAAACCAAAACCTCAGGAACCATAAAAAAAAAAGGG   2213       Acyl-ACP-thioesterase   CATCAAAAATGTTG   T   AGCTTTCGTGTAATGTGACTAACAACTTACAC         Brassica napus     ACCTTCTCCTTCTTCTCCGATTCCTCCC       Lys3Term   GGGAGGAATCGGAGAAGAAGGAGAAGGTGTGTAAGTTGTTAGTCA   2214       AAG-TAG   CATTACACGAAAGCT   A   CAACATTTTTGATGCCCTTTTTTTTTTATGG           TTCCTGAGGTTTTGGTTTATAGAAGAAGA           AAATGTTG   T   AGCTTTCG   2215           CGAAAGCT   A   CAACATTT   2216               Reduced palmitate   CTATAAACCAAAACCTCAGGAACCATAAAAAAAAAAGGGCATCAAA   2217       Acyl-ACP-thioesterase   AATGTTGAAGCTTT   A   GTGTAATGTGACTAACAACTTACACACCTTCT         Brassica napus     CCTTCTTCTCCGATTCCTCCCTTTTCAT       Ser5Term   ATGAAAAGGGAGGAATCGGAGAAGAAGGAGAAGGTGTGTAAGTT   2218       TCG-TAG   GTTAGTCACATTACAC   T   AAAGCTTCAACATTTTTGATGCCCTTTTTT           TTTTATGGTTCCTGAGGTTTTGGTTTATAG           GAAGCTTT   A   GTGTAATG   2219           CATTACAC   T   AAAGCTTC   2220               Reduced palmitate   AAACCAAAACCTCAGGAACCATAAAAAAAAAAGGGCATCAAAAATG   2221       Acyl-ACP-thioesterase   TTGAAGCTTTCGTG   A   AATGTGACTAACAACTTACACACCTTCTCCTT         Brassica napus     CTTCTCCGATTCCTCCCTTTTCATCCCG       Cys6Term   CGGGATGAAAAGGGAGGAATCGGAGAAGAAGGAGAAGGTGTGTA   2222       TGT-TGA   AGTTGTTAGTCACATT   T   CACGAAAGCTTCAACATTTTTGATGCCCTT           TTTTTTTTATGGTTCCTGAGGTTTTGGTTT           CTTTCGTG   A   AATGTGAC   2223           GTCACATT   T   CACGAAAG   2224                  
 
     [0147]                   TABLE 23                          Oligonucleotides to produce plants with increased stearate                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               Increased stearate   GGGAGAGCTCTAGCTCTGTAGAAAAGAAGGATTCATTCATCATATC   2225           stearoyl-ACP   CAGAAATGGCTCTA   T   AGTTTAACCCTTTGGTGGCATCTCAGCCTTA       desaturase   CAAATTCCCTTCCTCGACTCGTCCGCCAA         Arabidopsis thaliana     TTGGCGGACGAGTCGAGGAAGGGAATTTGTAAGGCTGAGATGCC   2226       Lys4 Term   ACCAAAGGGTTAAACT   A   TAGAGCCATTTCTGGATATGATGAATGAA       AAG-TAG   TCCTTCTTTTCTACAGAGCTAGAGCTCTCCC           TGGCTCTA   T   AGTTTAAC   2227           GTTAAACT   A   TAGAGCCA   2228               Increased stearate   CTCTGTAGAAAAGAAGGATTCATTCATCATATCCAGAAATGGCTCT   2229       stearoyl-ACP   AAAGTTTAACCCTT   A   GGTGGCATCTCAGCCTTACAAATTCCCTTCC       desaturase   TCGACTCGTCCGCCAACTCCTCTTTCAG         Arabidopsis thaliana     CTGAAAGAAGGAGTTGGCGGACGAGTCGAGGAAGGGAATTTGTA   2230       Leu8 Term   AGGCTGAGATGCCACC   T   AAGGGTTAAACTTTAGAGCCATTTCTGG       TTG-TAG   ATATGATGAATGAATCCTTCTTTTCTACAGAG           TAACCCTT   A   GGTGGCAT   2231           ATGCCACC   T   AAGGGTTA   2232               Increased stearate   AGAAGGATTCATTCATCATATCCAGAAATGGCTCTAAAGTTTAACC   2233       stearoyl-ACP   CTTTGGTGGCATCT   T   AGCCTTACAAATTCCCTTCCTCGACTCGTCC       desaturase   GCCAACTCCTTCTTTCAGATCTCCCAAGT         Arabidopsis thaliana     ACTTGGGAGATCTGAAAGAAGGAGTTGGCGGACGAGTCGAGGAA   2234       Gln12 Term   GGGAATTTGTAAGGCT   A   AGATGCCACCAAAGGGTTAAACTTTAGA       CAG-TAG   GCCATTTCTGGATATGATGAATGAATCCTTCT           TGGCATCT   T   AGCCTTAC   2235           GTAAGGCT   A   AGATGCCA   2236               Increased stearate   TCATTCATCATATCCAGAAATGGCTCTAAAGTTTAACCCTTTGGTG   2237       stearoyl-ACP   GCATCTCAGCCTTA   G   AAATTCCCTTCCTCGACTCGTCCGCCAACTC       desaturase   CTTCTTTCAGATCTCCCAAGTTCCTCTGC         Arabidopsis thaliana     GCAGAGGAACTTGGGAGATCTGAAAGAAGGAGTTGGCGGACGAG   2238       Phe14 Term   TCGAGGAAGGGAATTT   C   TAAGGCTGAGATGCCACCAAAGGGTTAA       TAC-TAG   ACTTTAGAGCCATTTCTGGATATGATGAATGA           CAGCCTTA   G   AAATTCCC   2239           GGGAATTT   C   TAAGGCTG   2240               Increased stearate   GAGAGCTCGCTCGTGTCTGAAAGAACATCAAACCTCGTATCAAAAA   2241       stearoyl-ACP   AAAGAAAATGGCAT   A   GAAGCTTAACCCTTTGGCATCTCAGCCTTAC       desaturase   AAACTCCCTTCCTCGGCTCGTCCGCCAAT         Brassica napus     ATTGGCGGACGAGCCGAGGAAGGGAGTTTGTAAGGCTGAGATGC   2242       Leu3 Term   CAAAGGGTTAAGCTTC   T   ATGCCATTTTCTTTTTTTTGATACGAGGTT       TTG-TAG   TGATGTTCTTTCAGACACGAGCGAGCTCTC           AATGGCAT   A   GAAGCTTA   2243           TAAGCTTC   T   ATGCCATT   2244               Increased stearate   GAGCTCGCTCGTGTCTGAAAGAACATCAAACCTCGTATCAAAAAAA   2245       stearoyl-ACP   AGAAAATGGCATTG   T   AGCTTAACCCTTTGGCATCTCAGCCTTACAA       desaturase   ACTCCCTTCCTCGGCTCGTCCGCCAATCT         Brassica napus     AGATTGGCGGACGAGCCGAGGAAGGGAGTTTGTAAGGCTGAGAT   2246       Lys4 Term   GCCAAAGGGTTAAGCT   A   CAATGCCATTTTCTTTTTTTTGATACGAG       AAG-TAG   GTTTGATGTTCTTTCAGACACGAGCGAGCTC           TGGCATTG   T   AGCTTAAC   2247           GTTAAGCT   A   CAATGCCA   2248               Increased stearate   TCTGAAAGAACATCAAACCTCGTATCAAAAAAAAGAAAATGGCATT   2249       stearoyl-ACP   GAAGCTTAACCCTT   A   GGCATCTCAGCCTTACAAACTCCCTTCCTCG       desaturase   GCTCGTCCGCCAATCTCTACTCTCAGATC         Brassica napus     GATCTGAGAGTAGAGATTGGCGGACGAGCCGAGGAAGGGAGTTT   2250       Leu8 Term   GTAAGGCTGAGATGCC   T   AAGGGTTAAGCTTCAATGCCATTTTCTTT       TTG-TAG   TTTTTGATACGAGGTTTGATGTTCTTTCAGA           TAACCCTT   A   GGCATCTC   2251           GAGATGCC   T   AAGGGTTA   2252               Increased stearate   AACATCAAACCTCGTATCAAAAAAAAGAAAATGGCATTGAAGCTTA   2253       stearoyl-ACP   ACCCTTTGGCATCT   T   AGCCTTACAAACTCCCTTCCTCGGCTCGTCC       desaturase   GCCAATCTCTACTCTCAGATCTCCCAAGT         Brassica napus     ACTTGGGAGATCTGAGAGTAGAGATTGGCGGACGAGCCGAGGAA   2254       Gln11 Term   GGGAGTTTGTAAGGCT   A   AGATGCCAAAGGGTTAAGCTTCAATGCC       CAG-TAG   ATTTTCTTTTTTTTGATACGAGGTTTGATGTT           TGGCATCT   T   AGCCTTAC   2255           GTAAGGCT   A   AGATGCCA   2256               Increased stearate   AACCAAAAGAAAAGGTAAGAAAAAAAACAATGGCTCTCAAGCTCA   2257       stearoyl-ACP   ATCCTTTCCTTTCT   T   AAACCCAAAAGTTACCTTCTTTCGCTCTTCCA       desaturase   CCAATGGCCAGTACCAGATCTCCTAAGT         Ricinus communis     ACTTAGGAGATCTGGTACTGGCCATTGGTGGAAGAGCGAAAGAAG   2258       Gln27 Term   GTAACTTTTGGGTTT   A   AGAAAGGATTGAGCTTGAGAGCCAT       CAA-TAA   TGTTTTTTTTCTTACCTTTTTCTTTTGGTT           TCCTTTCT   T   AAACCCAA   2259           TTGGGTTT   A   AGAAAGGA   2260               Increased stearate   AAGAAAAAGGTAAGAAAAAAAACAATGGCTCTCAAGCTCAATCCTT   2261       stearoyl-ACP   TCCTTTCTCAAACC   T   AAAAGTTACCTTCTTTCGCTCTTCCACCAATG       desaturase   GCCAGTACCAGATCTCCTAAGTTCTACA         Ricinus communis     TGTAGAACTTAGGAGATCTGGTACTGGCCATTGGTGGAAGAGCGA   2262       Gln29 Term   AAGAAGGTAACTTTT   A   GGTTTGAGAAAGGAAAGGATTGAGCTTGA       CAA-TAA   GAGCCATTGTTTTTTTTCTTACCTTTTTCTT           CTCAAACC   T   AAAAGTTA   2263           TAACTTTT   A   GGTTTGAG   2264               Increased stearate   AAAAAGGTAAGAAAAAAAACAATGGCTCTCAAGCTCAATCCTTTCC   2265       stearoyl-ACP   TTTCTCAAACCCAA   T   AGTTACCTTCTTTCGCTCTTCCACCAATGGCC       desaturase   AGTACCAGATCTCCTAAGTTCTACATGG         Ricinus communis     CCATGTAGAACTTAGGAGATCTGGTACTGGCCATTGGTGGAAGAG   2266       Lys30 TermCGAAAGAAGGTAACT   A   TTGGGTTTGAGAAAGGAAAGGATTGAGCT       AAG-TAG   TGAGAGCCATTGTTTTTTTTCTTACCTTTTT           AAACCCAA   T   AGTTACCT   2267           AGGTAACT   A   TTGGGTTT   2268               Increased stearate   TCTCAAACCCAAAAGTTACCTTCTTTCGCTCTTCCACCAATGGCCA   2269       stearoyl-ACP   GTACCAGATCTCCT   T   AGTTCTACATGGCCTCTACCCTCAAGTCTGG       desaturase   TTCTAAGGAAGTTGAGAATCTCAAGAAGC         Ricinus communis     GCTTCTTGAGATTCTCAACTTCCTTAGAACCAGACTTGAGGGTAGA   2270       Lys46 Term   GGCCATGTAGAACT   A   AGGAGATCTGGTACTGGCCATTGGTGGAG       AAG-TAG   AGCGAAAGAAGGTAACTTTTGGGTTTGAGA           GATCTCCT   T   AGTTCTAC   2271           GTAGAACT   A   AGGAGATC   2272               Increased stearate   TCTTCTGATTCATTTAATCTTTACTCATCAATGGCTCTGAGACTGAA   2273       stearoyl-ACP   CCCTATCCCCACC   T   AAACCTTCTCCCTCCCCCAAATGGCCAGTCTC       desaturase   AGATCTCCCAGGTTCCGCATGGCCTCTA         Glycine max     TAGAGGCCATGCGGAACCTGGGAGATCTGAGACTGGCCATTTGG   2274       Gln11 Term   GGGAGGGAGAAGGTTT   A   GGTGGGGATAGGGTTCAGTCTCAGAGC       CAA-TAA   CATTGATGAGTAAAGATTAAATGAATCAGAAGA           TCCCCACC   T   AAACCTTC   2275           GAAGGTTT   A   GGTGGGGA   2276               Increased stearate   CTTTACTCATCAATGGCTCTGAGACTGAACCCTATCCCCACCCAAA   2277       stearoyl-ACP   CCTTCTCCCTCCCC   T   AAATGGCCAGTCTCAGATCTCCCAGGTTCC       desaturase   GCATGGCCTCTACCCTCCGCTCCGGTTCCA         Glycine max     TGGAACCGGAGCGGAGGGTAGAGGCCATGCGGAACCTGGGAGAT   2278       Gln17 Term   CTGAGACTGGCCATTT   A   GGGGAGGGAGAAGGTTTGGGTGGGGAT       CAA-TAA   AGGGTTCAGTCTCAGAGCCATTGATGAGTAAAG           CCCTCCCC   T   AAATGGCC   2279           GGCCATTT   A   GGGGAGGG   2280               Increased stearate   GCTCTGAGACTGAACCCTATCCCCACCCAAACCTTCTCCCTCCCC   2281       stearoyl-ACP   CAAATGGCCAGTCTC   T   GATCTCCCAGGTTCCGCATGGCCTCTACC       desaturase   CTCCGCTCCGGTTCCAAAGAGGTTGAAAATA         Glycine max     TATTTTCAACCTCTTTGGAACCGGAGCGGAGGGTAGAGGCCATGC   2282       Arg22 Term   GGAACCTGGGAGATC   A   GAGACTGGCCATTTGGGGGAGGGAGAAG       AGA-TGA   GTTTGGGTGGGGATAGGGTTCAGTCTCAGAGC           CCAGTCTC   T   GATCTCCC   2283           GGGAGATC   A   GAGACTGG   2284               Increased stearate   CAAATGGCCAGTCTCAGATCTCCCAGGTTCCGCATGGCCTCTACC   2285       stearoyl-ACP   CTCCGCTCCGGTTCC   T   AAGAGGTTGAAAATATTAAGAAGCCATTCA       desaturase   CTCCTCCCAGAGAAGTGCATGTTCAAGTAA         Glycine max     TTACTTGAACATGCACTTCTCTGGGAGGAGTGAATGGCTTCTTAAT   2286       Lys37 Term   ATTTTCAACCTCTT   A   GGAACCGGAGCGGAGGGTAGAGGCCATGCG       AAA-TAA   GAACCTGGGAGATCTGAGACTGGCCATTTG           CCGGTTCC   T   AAGAGGTT   2287           AACCTCTT   A   GGAACCGG   2288               Increased stearate   CAACAAGCACACACAAGAACAACATCAACAATGGCGATTCGCATC   2289       stearoyl-ACP   AATACGGCGACGTTT   T   AATCAGACCTGTACCGTTCATTCGCGTTTC       desaturase   CTCAACCGAAACCTCTCAGATCTCCCAAAT         Helianthus annuus     ATTTGGGAGATCTGAGAGGTTTCGGTTGAGGAAACGCGAATGAAC   2290       Gln11 Term   GGTACAGGTCTGATT   A   AAACGTCGCCGTATTGATGCGAATCGCCA       CAA-TAA   TTGTTGATGTTGTTCTTGTGTGTGCTTGTTG           CGACGTTT   T   AATCAGAC   2291           GTCTGATT   A   AAACGTCG   2292               Increased stearate   AAGCACACACAAGAAGCAACATCAACAATGGCGATTCGCATCAATAC   2293       stearoyl-ACP   GGCGACGTTTCAAT   G   AGACCTGTACCGTTCATTCGCGTTTCCTCAA       desaturase   CCGAAACCTCTCAGATCTCCCAAATTCGC         Helianthus annuus     GCGAATTTGGGAGATCTGAGAGGTTTCGGTTGAGGAAACGCGAAT   2294       Ser12 Term   GAACGGTACAGGTCT   C   ATTGAAACGTCGCCGTATTGATGCGAATC       TCA-TGA   GCCATTGTTGATGTTGTTCTTGTGTGTGCTT           GTTTCAAT   G   AGACCTGT   2295           ACAGGTCT   C   ATTGAAAC   2296               Increased stearate   AAGAACAACATCAACAATGGCGATTCGCATCAATACGGCGACGTTT   2297       stearoyl-ACP   CAATCAGACCTGTA   G   CGTTCATTCGCGTTTCCTCAACCGAAACCTC       desaturase   TCAGATCTCCCAAATTCGCCATGGCTTCC         Helianthus annuus     GGAAGCCATGGCGAATTTGGGAGATCTGAGAGGTTTCGGTTGAGG   2298       Tyr15 Term   AAACGCGAATGAACG   C   TACAGGTCTGATTGAAACGTCGCCGTATT       TAC-TAG   GATGCGAATCGCCATTGTTGATGTTGTTCTT           GACCTGTA   G   CGTTCATT   2299           AATGAACG   C   TACAGGTC   2300               Increased stearate   CAACATCAACAATGGCGATTCGCATCAATACGGCGACGTTTCAATC   2301       stearoyl-ACP   AGACCTGTACCGTT   G   ATTCGCGTTTCCTCAACCGAAACCTCTCAGA       desaturase   TCTCCCAAATTCGCCATGGCTTCCACCAT         Helianthus annuus     ATGGTGGAAGCCATGGCGAATTTGGGAGATCTGAGAGGTTTCGGT   2302       Ser17 Term   TGAGGAAACGCGAAT   C   AACGGTACAGGTCTGATTGAAACGTCGCC       TCA-TGA   GTATTGATGCGAATCGCCATTGTTGATGTTG           GTACCGTT   G   ATTCGCGT   2303           ACGCGAAT   C   AACGGTAC   2304               Increased stearate   ACACACAACACACACTCAATCACACACACATCATCATCTTCTTCATC   2305       stearoyl-ACP   AACGATGGCGCTT   T   GAATGAGTCCGGTGACGCTTCAACGGGAGAT       desaturase   ATATCCTTCATACACTTTTCATCAATCGA         Helianthus annuus     TCGATTGATGAAAAGTGTATGAAGGATATATCTCCCGTTGAAGCGT   2306       Arg4 Term   CACCGGACTCATTC   A   AAGCGCCATCGTTGATGAAGAAGATGATGA       CGA-TGA   TGTGTGTGTGATTGAGTGTGTGTTGTGTGT           TGGCGCTT   T   GAATGAGT   2307           ACTCATTC   A   AAGCGCCA   2308               Increased stearate   ACACACACATCATCATCTTCTTCATCAACGATGGCGCTTCGAATGA   2309       stearoyl-ACP   GTCCGGTGACGCTT   T   AACGGGAGATATATCCTTCATACACTTTTCA       desaturase   TCAATCGAAAAATCTCAGATCTCCTAAAT         Helianthus annuus     ATTTAGGAGATCTGAGATTTTTCGATTGATGAAAAGTGTATGAAGG   2310       Gln11 Term   ATATATCTCCCGTT   A   AAGCGTCACCGGACTCATTCGAAGCGCCATC       CAA-TAA   GTTGATGAAGAAGATGATGATGTGTGTGT           TGACGCTT   T   AACGGGAG   2311           CTCCCGTT   A   AAGCGTCA   2312               Increased stearate   ACATCATCATCTTCTTCATCAACGATGGCGCTTCGAATGAGTCCGG   2313       stearoyl-ACP   TGACGCTTCAACGG   T   AGATATATCCTTCATACACTTTTCATCAATCG       desaturase   AAAAATCTCAGATCTCCTAAATTCGCGA         Helianthus annuus     TCGCGAATTTAGGAGATCTGAGATTTTTCGATTGATGAAAAGTGTA   2314       Glu13 Term   TGAAGGATATATCT   A   CCGTTGAAGCGTCACCGGACTCATTCGAAG       GAG-TAG   CGCCATCGTTGATGAAGAAGATGATGATGT           TTCAACGG   T   AGATATAT   2315           ATATATCT   A   CCGTTGAA   2316               Increased stearate   ATCTTCTTCATCAACGATGGCGCTTCGAATGAGTCCGGTGACGCTT   2317       stearoyl-ACP   CAACGGGAGATATA   G   CCTTCATACACTTTTCATCAATCGAAAAATC       desaturase   TCAGATCTCCTAAATTCGCGATGGCTTCC         Helianthus annuus     GGAAGCCATCGCGAATTTAGGAGATCTGAGATTTTTCGATTGATGA   2318       Tyr15 Term   AAAGTGTATGAAGG   C   TATATCTCCCGTTGAAGCGTCACCGGACTC       TAT-TAG   ATTCGAAGCGCCATCGTTGATGAAGAAGAT           GAGATATA   G   CCTTCATA   2319           TATGAAGG   C   TATATCTC   2320               Increased stearate   AACTCAGCCAGCTTGCCCCCAAACAACAGCGCAGAAAAACCTTCA   2321       stearoyl-ACP   ACAACAATGGCTCTC   T   AGCTCAACCCAGTCACCACCTTCCCTTCAA       desaturase   CACGCTCCCTCAACAACTTCTCCTCCAGAT         Linum usitatissimum     ATCTGGAGGAGAAGTTGTTGAGGGAGCGTGTTGAAGGGAAGGTG   2322       Lys4 Term   GTGACTGGGTTGAGCT   A   GAGAGCCATTGTTGTTGAAGGTTTTTCT       AAG-TAG   GCGCTGTTGTTTGGGGGCAAGCTGGCTGAGTT           TGGCTCTC   T   AGCTCAAC   2323           GTTGAGCT   A   GAGAGCCA   2324               Increased stearate   GCGCAGAAAAACCTTCAACAACAATGGCTCTCAAGCTCAACCCAG   2325       stearoyl-ACP   TCACCACCTTCCCTT   G   AACACGCTCCCTCAACAACTTCTCCTCCAG       desaturase   ATCTCCTCGCACCTTTCTCATGGCTGCTTC         Linum usitatissimum     GAAGCAGCCATGAGAAAGGTGCGAGGAGATCTGGAGGAGAAGTT   2326       Ser13 Term   GTTGAGGGAGCGTGTT   C   AAGGGAAGGTGGTGACTGGGTTGAGCT       TCA-TGA   TGAGAGCCATTGTTGTTGAAGGTTTTTCTGCGC           CTTCCCTT   G   AACACGCT   2327           AGCGTGTT   C   AAGGGAAG   2328               Increased stearate   CTCAAGCTCAACCCAGTCACCACCTTCCCTTCAACACGCTCCCTCA   2329       stearoyl-ACP   ACAACTTCTCCTCC   T   GATCTCCTCGCACCTTTCTCATGGCTGCTTC       desaturase   CACTTTCAATTCCACCTCCACCAAGTAAG         Linum usitatissimum     CTTACTTGGTGGAGGTGGAATTGAAAGTGGAAGCAGCCATGAGAA   2330       Arg23 Term   AGGTGCGAGGAGATC   A   GGAGGAGAAGTTGTTGAGGGAGCGTGTT       AGA-TGA   GAAGGGAAGGTGGTGACTGGGTTGAGCTTGAG           TCTCCTCC   T   GATCTCCT   2331           AGGAGATC   A   GGAGGAGA   2332               Increased stearate   TCCTCCAGATCTCCTCGCACCTTTCTCATGGCTGCTTCCACTTTCA   2333       stearoyl-ACP   ATTCCACCTCCACC   T   AGTAAGCATCTCCTCCTCCTCGGAATCTCCG       desaturase   CCGATTTCTTTTAAGCGATTGATCGTAGA         Linum usitatissimum     TCTACGATCAATCGCTTAAAAGAAATCGGCGGAGATTCCGAGGAG   2334       Lys411 Term   GAGGAGATGCTTACT   A   GGTGGAGGTGGAATTGAAAGTGGAAGCA       AAG-TAG   GCCATGAGAAAGGTGCGAGGAGATCTGGAGGA           CCTCCACC   T   AGTAAGCA   2335           TGCTTACT   A   GGTGGAGG   2336               Increased stearate   ATGGCACTGAAACTTTGCTTTCCACCCCACAAGATGCCTTCCTTCC   2337       stearoyl-ACP   CCGATGCTCGTATC   T   GATCTCACAGGGTTTTCATGGCTTCAACTAT       desaturase   TCATTCTCCTTCTATGGAGGTCGGAAAAG         Olea europaeap     CTTTCCGACCTCCATAGAAGGAGAATGAATAGTTGAAGCCATGAA   2338       Arg21 Term   AACCCTGTGAGATC   A   GATACGAGCATCGGGGAAGGAAGGCATCTT       AGA-TGA   GTGGGGTGGAAAGCAAAGTTTCAGTGCCAT           CTCGTATC   T   GATCTCAC   2339           GTGAGATC   A   GATACGAG   2340               Increased stearate   CCCACAAGATGCCTTCCTTCCCCGATGCTCGTATCAGATCTCACAG   2341       stearoyl-ACP   GGTTTTCATGGCTT   G   AACTATTCATTCTCCTTCTATGGAGGTCGGA       desaturase   AAAGTTAAAAAGCCTTTCACGCCTCCACG         Olea europaeap     CGTGGAGGCGTGAAAGGCTTTTTAACTTTTCCGACCTCCATAGAA   2342       Ser29 Term   GGAGAATGAATAGTT   C   AAGCCATGAAAACCCTGTGAGATCTGATAC       TCA-TGA   GAGCATCGGGGAAGGAAGGCATCTTGTGGG           CATGGCTT   G   AACTATTC   2343           GAATAGTT   C   AAGCCATG   2344               Increased stearate   GATGCTCGTATCAGATCTCACAGGGTTTTCATGGCTTCAACTATTC   2345       stearoyl-ACP   ATTCTCCTTCTATG   T   AGGTCGGAAAAGTTAAAAAGCCTTTCACGCC       desaturase   TCCACGAGAGGTACATGTTCAAGTAACCC         Olea europaeap     GGGTTACTTGAACATGTACCTCTCGTGGAGGCGTGAAAGGCTTTT   2346       Glu37 Term   TAACTTTTCCGACCT   A   CATGAAGGAGAATGAATAGTTGAAGCCAT       GAG-TAG   GAAAACCCTGTGAGATCTGATACGAGCATC           CTTCTATG   T   AGGTCGGA   2347           TCCGACCT   A   CATAGAAG   2348               Increased stearate   CGTATCAGATCTCACAGGGTTTTCATGGCTTCAACTATTCATTCTC   2349       stearoyl-ACP   CTTCTATGGAGGTC   T   GAAAAGTTAAAAAGCCTTTCACGCCTCCACG       desaturase   AGAGGTACATGTTCAAGTAACCCATTCCT         Olea europaeap     AGGAATGGGTTACTTGAACATGTACCTCTCGTGGAGGCGTGAAAG   2350       Gly39 Term   GCTTTTTAACTTTTC   A   GACCTCCATAGAAGGAGAATGAATAGTTGA       GGA-TGA   AGCCATGAAAACCCTGTGAGATCTGATACG           TGGAGGTC   T   GAAAAGTT   2351           AACTTTTC   A   GACCTCCA   2352               Increased stearate   TTCTCGTTTTTGTCGTCCCCTCTGCTCTCTCTCTCTATCAGGCACG   2353       stearoyl-ACP   GAGAAATGGCACTG   T   AACTCAGTCCAGTCATGTTTCAATCTCAGAA       desaturase   GCTTCCATTTCTTGCCTCCTATCCGCCTT         Persea americana     AAGGCGGATAGGAGGCAAGAAATGGAAGCTTCTGAGATTGAAACA   2354       Lys4 Term   TGACTGGACTGAGTT   A   CAGTGCCATTTCTCCGTGCCTGATAGAGA       AAA-TAA   GAGAGAGCAGAGGGGACGACAAAAACGAGAA           TGGCACTG   T   AACTCAGT   2355           ACTGAGTT   A   CAGTGCCA   2356               Increased stearate   CTGCTCTCTCTCTCTATCAGGCACGGAGAAATGGCACTGAAACTCA   2357       stearoyl-ACP   GTCCAGTCATGTTT   T   AATCTCAGAAGCTTCCATTTCTTGCCTCCTAT       desaturase   CCGCCTTCCAATCTCAGATCTCCGAGGG         Persea americana     CCCTCGGAGATCTGAGATTGGAAGGCGGATAGGAGGCAAGAAAT   2358       Gln11 Term   GGAAGCTTCTGAGATT   A   AAACATGACTGGACTGAGTTTCAGTGCC       CAA-TAA   ATTTCTCCGTGCCTGATAGAGAGAGAGAGCAG           TCATGTTT   T   AATCTCAG   2359           CTGAGATT   A   AAACATGA   2360               Increased stearate   TCTCTCTCTATCAGGCACGGAGAAATGGCACTGAAACTCAGTCCA   2361       stearoyl-ACP   GTCATGTTTCAATCT   T   AGAAGCTTCCATTTCTTGCCTCCTATCCGCC       desaturase   TTCCAATCTCAGATCTCCGAGGGTTTTCA         Persea americana     TGAAAACCCTCGGAGATCTGAGATTGGAAGGCGGATAGGAGGCAA   2362       Gln13 Term   GAAATGGAAGCTTCT   A   AGATTGAAACATGACTGGACTGAGTTTCAG       CAG-TAG   TGCCATTTCTCCGTGCCTGATAGAGAGAGA           TTCAATCT   T   AGAAGCTT   2363           AAGCTTCT   A   AGATTGAA   2364               Increased stearate   CTCTCTATCAGGCACGGAGAAATGGCACTGAAACTCAGTCCAGTC   2365       stearoyl-ACP   ATGTTTCAATCTCAG   T   AGCTTCCATTTCTTGCCTCCTATCCGCCTTC       desaturase   CAATCTCAGATCTCCGAGGGTTTTCATGG         Persea americana     CCATGAAAACCCTCGGAGATCTGAGATTGGAAGGCGGATAGGAG   2366       Lys14 Term   GCAAGAAATGGAAGCT   A   CTGAGATTGAAACATGACTGGACTGAGT       AAG-TAG   TTCAGTGCCATTTCTCCGTGCCTGATAGAGAG           AATCTCAG   T   AGCTTCCA   2367           TGGAAGCT   A   CTGAGATT   2368               Increased stearate   CCCCGAGATCTCGCTGCCGCTGCTCATGGCGTTCGCGGCGTCCC   2369       stearoyl-ACP   ACACCGCATCGCCGTA   G   TCCTGCGGCGGCGTGGCGCAGAGGAG       desaturase   GAGCAATGGGATGTCGAAGATGGTGGCCATGGCC         Oryza sativa     GGCCATGGCCACCATCTTCGACATCCCATTGCTCCTCCTCTGCGC   2370       Tyr12 Term   CACGCCGCCGCAGGA   C   TACGGCGATGCGGTGTGGGACGCCGCG       TAC-TAG   AACGCCATGAGCAGCGGCAGCGAGATCTCGGGG           TCGCCGTA   G   TCCTGCGG   2371           CCGCAGGA   C   TACGGCGA   2372               Increased stearate   CTGCTCATGGCGTTCGCGGCGTCCCACACCGCATCGCCGTACTCC   2373       stearoyl-ACP   TGCGGCGGCGTGGCG   T   AGAGGAGGAGCAATGGGATGTCGAAGAT       desaturase   GGTGGCCATGGCCTCCACCATCAACAGGGTCA         Oryza sativa     TGACCCTGTTGATGGTGGAGGCCATGGCCACCATCTTCGACATCC   2374       Gln19 Term   CATTGCTCCTCCTCT   A   CGCCACGCCGCCGCAGGAGTACGGCGAT       CAG-TAG   GCGGTGTGGGACGCCGCGAACGCCATGAGCAG           GCGTGGCG   T   AGAGGAGG   2375           CCTCCTCT   A   CGCCACGC   2376               Increased stearate   CCCACACCGCATCGCCGTACTCCTGCGGCGGCGTGGCGCAGAGG   2377       stearoyl-ACP   AGGAGCAATGGGATGT   A   GAAGATGGTGGCCATGGCCTCCACCAT       desaturase   CAACAGGGTCAAGACTGCTAAGAAGCCCTACAC         Oryza sativa     GTGTAGGGCTTCTTAGCAGTCTTGACCCTGTTGATGGTGGAGGCC   2378       Ser26 Term   ATGGCCACCATCTTC   T   ACATCCCATTGCTCCTCCTCTGCGCCACGC       TCG-TAG   CGCCGCAGGAGTACGGCGATGCGGTGTGGG           TGGGATGT   A   GAAGATGG   2379           CCATCTTC   T   ACATCCCA   2380               Increased stearate   CACACCGCATCGCCGTACTCCTGCGGCGGCGTGGCGCAGAGGAG   2381       stearoyl-ACP   GAGCAATGGGATGTCG   T   AGATGGTGGCCATGGCCTCCACCATCAA       desaturase   CAGGGTCAAGACTGCTAAGAAGCCCTACACTC         Oryza sativa     GAGTGTAGGGCTTCTTAGCAGTCTTGACCCTGTTGATGGTGGAGG   2382       Lys27 Term   CCATGGCCACCATCT   A   CGACATCCCATTGCTCCTCCTCTGCGCCA       AAG-TAG   CGCCGCCGCAGGAGTACGGCGATGCGGTGTG           GGATGTCG   T   AGATGGTG   2383           CACCATCT   A   CGACATCC   2384               Increased stearate   TTCTCTCTCTAGGTTGAGCGGTTACCAACAGAAGCACTTAGGAGA   2385       stearoyl-ACP   GAGAAGCAATGGCGT   A   GAAGCTTCACCACACGGCCTTCAATCCTT       desaturase   CCATGGCGGTTACCTCTTCGGGACTTCCTCG         Simmondsia chinensis     CGAGGAAGTCCCGAAGAGGTAACCGCCATGGAAGGATTGAAGGC   2386       Leu3 Term   CGTGTGGTGAAGCTTC   T   ACGCCATTGCTTCTCTCTCCTAAGTGCTT       TTG-TAG   CTGTTGGTAACCGCTCAACCTAGAGAGAGAA           AATGGCGT   A   GAAGCTTC   2387           GAAGCTTC   T   ACGCCATT   2388               Increased stearate   CTCTCTCTAGGTTGAGCGGTTACCAACAGAAGCACTTAGGAGAGA   2389       stearoyl-ACP   GAGCAATGGCGTTG   T   AGCTTCACCACACGGCCTTCAATCCTTCC       desaturase   ATGGCGGTTACCTCTTCGGGACTTCCTCGAT         Simmondsia chinensis     ATCGAGGAAGTCCCGAAGAGGTAACCGCCATGGAAGGATTGAAG   2390       Lys4 Term   GCCGTGTGGTGAAGCT   A   CAACGCCATTGCTTCTCTCTCCTAAGTG       AAG-TAG   CTTCTGTTGGTAACCGCTCAACCTAGAGAGAG           TGGCGTTG   T   AGCTTCAC   2391           GTGAAGCT   A   CAACGCCA   2392               Increased stearate   AAGCAATGGCGTTGAAGCTTCACCACACGGCCTTCAATCCTTCCAT   2393       stearoyl-ACP   GGCGGTTACCTCTT   A   GGGACTTCCTCGATCGTATCACCTCAGATCT       desaturase   CACCGCGTTTTCATGGCTTCTTCTACAAT         Simmondsia chinensis     ATTGTAGAAGAAGCCATGAAAACGCGGTGAGATCTGAGGTGATAC   2394       Ser19 Term   GATCGAGGAAGTCCC   T   AAGAGGTAACCGCCATGGAAGGATTGAAG       TCG-TAG   GCCGTGTGGTGAAGCTTCAACGCCATTGCTT           TACCTCTT   A   GGGACTTC   2395           GAAGTCCC   T   AAGAGGTA   2396               Increased stearate   GCAATGGCGTTGAAGCTTCACCACACGGCCTTCAATCCTTCCATG   2397       stearoyl-ACP   GCGGTTACCTCTTCG   T   GACTTCCTCGATCGTATCACCTCAGATCTC       desaturase   ACCGCGTTTTCATGGCTTCTTCTACAATTG         Simmondsia chinensis     CAATTGTAGAAGAAGCCATGAAAACGCGGTGAGATCTGAGGTGAT   2398       Gly20 Term   ACGATCGAGGAAGTC   A   CGAAGAGGTAACCGCCATGGAAGGATTG       GGA-TGA   AAGGCCGTGTGGTGAAGCTTCAACGCCATTGC           CCTCTTCG   T   GACTTCCT   2399           AGGAAGTC   A   CGAAGAGG   2400               Increased stearate   TGGCTCTGAATCTCAACCCCGTTTCCACACCATTTCAGTGTCGTCG   2401       stearoyl-ACP   ATTGCCGTCTTTCT   G   ACCTCGTCAAACGCCTTCTCGCAGATCTCCC       desaturase   AAATTCTTCATGGCTTCCACTCTCAGCAG         Spinacia oleracea     CTGCTGAGAGTGGAAGCCATGAAGAATTTGGGAGATCTGCGAGAA   2402       Ser21 Term   GGCGTTTGACGAGGT   C   AGAAAGACGGCAATCGACGACACTGAAAT       TCA-TGA   GGTGTGGAAACGGGGTTGAGATTCAGAGCCA           GTCTTTCT   G   ACCTCGTC   2403           GACGAGGT   C   AGAAAGAC   2404               Increased stearate   AATCTCAACCCCGTTTCCACACCATTTCAGTGTCGTCGATTGCCGT   2405       stearoyl-ACP   CTTTCTCACCTCGT   T   AAACGCCTTCTCGCAGATCTCCCAAATTCTT       desaturase   CATGGCTTCCACTCTCAGCAGCTCTTCTC         Spinacia oleracea     GAGAAGAGCTGCTGAGAGTGGAAGCCATGAAGAATTTGGGAGATC   2406       Gln24 Term   TGCGAGAAGGCGTTT   A   ACGAGGTGAGAAAGACGGCAATCGACGA       CAA-TAA   CACTGAAATGGTGTGGAAACGGGGTTGAGATT           CACCTCGT   T   AAACGCCT   2407           AGGCGTTT   A   ACGAGGTG   2408               Increased stearate   TCCACACCATTTCAGTGTCGTCGATTGCCGTCTTTCTCACCTCGTC   2409       stearoyl-ACP   AAACGCCTTCTCGC   T   GATCTCCCAAATTCTTCATGGCTTCCACTCT       desaturase   CAGCAGCTCTTCTCCTAAGGAAGCGGAAA         Spinacia oleracea     TTTCCGCTTCCTTAGGAGAAGAGCTGCTGAGAGTGGAAGCCATGA   2410       Arg29 Term   AGAATTTGGGAGATC   A   GCGAGAAGGCGTTTGACGAGGTGAGAAA       AGA-TGA   GACGGCAATCGACGACACTGAAATGGTGTGGA           CTTCTCGC   T   GATCTCCC   2411           GGGAGATC   A   GCGAGAAG   2412               Increased stearate   TTTCAGTGTCGTCGATTGCCGTCTTTCTCACCTCGTCAAACGCCTT   2413       stearoyl-ACP   CTCGCAGATCTCCC   T   AATTCTTCATGGCTTCCACTCTCAGCAGCTC       desaturase   TTCTCCTAAGGAAGCGGAAAGCCTGAAGA         Spinacia oleracea     TCTTCAGGCTTTCCGCTTCCTTAGGAGAAGAGCTGCTGAGAGTGG   2414       Lys32 Term   AAGCCATGAAGAATT   A   GGGAGATCTGCGAGAAGGCGTTTGACGAG       AAA-TAA   GTGAGAAAGACGGCAATCGACGACACTGAAA           GATCTCCC   T   AATTCTTC   2415           GAAGAATT   A   GGGAGATC   2416               Increased stearate   AAATAGTCGAGGTGAAAAACAGAGCATCAACAATGGCACTGAATAT   2417       stearoyl-ACP   CAATGGGGTGTCGT   G   AAAATCTCACAAAATGTTACCATTTCCTTGT       desaturase   TCTTCAGCCAGATCTGAGCGAGTTTTCAT         Solanum tuberosum     ATGAAAACTCGCTCAGATCTGGCTGAAGAACAAGGAAATGGTAAC   2418       Leu10 Term   ATTTTGTGAGATTTT   C   ACGACACCCCATTGATATTCAGTGCCATTGT       TTA-TGA   TGATGCTCTGTTTTTCACCTCGACTATTT           GGTGTCGT   G   AAAATCTC   2419           GAGATTTT   C   ACGACACC   2420               Increased stearate   ATAGTCGAGGTGAAAACAGAGCATCAACAATGGCACTGAATATCA   2421       stearoyl-ACP   ATGGGGTGTCGTTA   T   AATCTCACAAAATGTTACCATTTCCTTGTTCT       desaturase   TCAGCCAGATCTGAGCGAGTTTTCATGG         Solanum tuberosum     CCATGAAAACTCGCTCAGATCTGGCTGAAGAACAAGGAAATGGTA   2422       Lys11 Term   ACATTTTGTGAGATT   A   TAACGACACCCCATTGATATTCAGTGCCATT       AAA-TAA   GTTGATGCTCTGTTTTTCACCTCGACTAT           TGTCGTTA   T   AATCTCAC   2423           GTGAGATT   A   TAACGACA   2424               Increased stearate   GTGAAAAACAGAGCATCAACAATGGCACTGAATATCAATGGGGTG   2425       stearoyl-ACP   TCGTTAAAATCTCAC   T   AAATGTTACCATTTCCTTGTTCTTCAGCCAG       desaturase   ATCTGAGCGAGTTTTCATGGCTTCAACCA         Solanum tuberosum     TGGTTGAAGCCATGAAAACTCGCTCAGATCTGGCTGAAGAACAAG   2426       Lys14 Term   GAAATGGTAACATTT   A   GTGAGATTTTAACGACACCCCATTGATATT       AAA-TAA   CAGTGCCATTGTTGATGCTCTGTTTTTCAC           AATCTCAC   T   AAATGTTA   2427           TAACATTT   A   GTGAGATT   2428               Increased stearate   ACAGAGCATCAACAATGGCACTGAATATCAATGGGGTGTCGTTAAA   2429       stearoyl-ACP   ATCTCACAAAATGT   G   ACCATTTCCTTGTTCTTCAGCCAGATCTGAG       desaturase   CGAGTTTTCATGGCTTCAACCATTCATCG         Solanum tuberosum     CGATGAATGGTTGAAGCCATGAAAACTCGCTCAGATCTGGCTGAA   2430       Leu16 Term   GAACAAGGAAATGGT   C   ACATTTTGTGAGATTTTAACGACACCCCAT       TTA-TGA   TGATATTCAGTGCCATTGTTGATGCTCTGT           CAAAATGT   G   ACCATTTC   2431           GAAATGGT   C   ACATTTTG   2432               Increased stearate   TGGCTCTGAGGCTGAACCCTAACCCTTCACAGAAGCTCTTTCTCTC   2433       stearoyl-ACP   TCCTTCTTCATCAT   G   ATCTTCTTCTTCTTCATCGTTCTCGCTTCCTC       desaturase   AAATGGCTAGCCTCAGATCTCCAAGGTT         Arachis hypogaea     AACCTTGGAGATCTGAGGCTAGCCATTTGAGGAAGCGAGAACGAT   2434       Ser21 Term   GAAGAAGAAGAAGAT   C   ATGATGAAGAAGGAGAGAGAAAGAGCTTC       TCA-TGA   TGTGAAGGGTTAGGGTTCAGCCTCAGAGCCA           TTCATCAT   G   ATCTTCTT   2435           AAGAAGAT   C   ATGATGAA   2436               Increased stearate   ACCCTAACCCTTCACAGAAGCTCTTTCTCTCTCCTTCTTCATCATCA   2437       stearoyl-ACP   TCTTCTTCTTCTT   G   ATCGTTCTCGCTTCCTCAAATGGCTAGCCTCA       desaturase   GTCTCCAAGGTTCCGCATGGCCTCCAC         Arachis hypogaea     GTGGAGGCCATGCGGAACCTTGGAGATCTGAGGCTAGCCATTTGA   2438       Ser26 Term   GGAAGCGAGAACGAT   C   AAGAAGAAGAAGATGATGATGAAGAAGGA       TCA-TGA   GAGAGAAAGAGCTTCTGTGAAGGGTTAGGGT           TTCTTCTT   G   ATCGTTCT   2439           AGAACGAT   C   AAGAAGAA   2440               Increased stearate   CTAACCCTTCACAGAAGCTCTTTCTCTCTCCTTCTTCATCATCATCT   2441       stearoyl-ACP   TCTTCTTCTTCAT   A   GTTCTCGCTTCCTCAAATGGCTAGCCTCAGAT       desaturase   CTCCAAGGTTCCGCATGGCCTCCACCCT         Arachis hypogaea     AGGGTGGAGGCCATGCGGAACCTTGGAGATCTGAGGCTAGCCAT   2442       Ser27 Term   TTGAGGAAGCGAGAAC   T   ATGAAGAAGAAGAAGATGATGATGAAGA       TCG-TAG   AGGAGAGAGAAAGAGCTTCTGTGAAGGGTTAG           TTCTTCAT   A   GTTCTCGC   2443           GCGAGAAC   T   ATGAAGAA   2444               Increased stearate   CTTCACAGAAGCTCTTTCTCTCTCCTTCTTCATCATCATCTTCTTCT   2445       stearoyl-ACP   TCTTCATCGTTCT   A   GCTTCCTCAAATGGCTAGCCTCAGATCTCCAA       desaturase   GGTTCCGCATGGCCTCCACCCTCCGCAC         Arachis hypogaea     GTGCGGAGGGTGGAGGCCATGCGGAACCTTGGAGATCTGAGGCT   2446       Ser29 Term   AGCCATTTGAGGAAGC   T   AGAACGATGAAGAAGAAGAAGATGATGA       TCG-TAG   TGAAGAAGGAGAGAGAAAGAGCTTCTGTGAAG           ATCGTTCT   A   GCTTCCTC   2447           GAGGAAGC   T   AGAACGAT   2448               Increased stearate   AAAGTTAAAAGCCGTCCAAAACCCAAACCAGGAAAGGCAAACGAA   2449       stearoyl-ACP   AAGAAAAAATGGCTT   A   GAATTTTAATGCCATCGCCTCGAAATCTCA       desaturase   GAAGCTCCCTTGCTTTGCTCTTCCACCAAA         Gossypium hirsutum     TTTGGTGGAAGAGCAAAGCAAGGGAGCTTCTGAGATTTCGAGGCG   2450       Leu3 Term   ATGGCATTAAAATTC   T   AAGCCATTTTTTCTTTTCGTTTGCCTTTCCT       TTG-TAG   GGTTTGGGTTTTGGACGGCTTTTAACTTT           AATGGCTT   A   GAATTTTA   2451           TAAAATTC   T   AAGCCATT   2452               Increased stearate   CCCAAACCAGGAAAGGCAAACGAAAAGAAAAAATGGCTTTGAATTT   2453       stearoyl-ACP   TAATGCCATCGCCT   A   GAAATCTCAGAAGCTCCCTTGCTTTGCTCTT       desaturase   CCACCAAAGGCCACCCTTAGATCTCCCAA         Gossypium hirsutum     TTGGGAGATCTAAGGGTGGCCTTTGGTGGAAGAGCAAAGCAAGG   2454       Ser1-Term   GAGCTTCTGAGATTTC   T   AGGCGATGGCATTAAAATTCAAAGCCATT       TCG-TAG   TTTTCTTTTCGTTTGCCTTTCCTGGTTTGGG           CATCGCCT   A   GAAATCTC   2455           GAGATTTC   T   AGGCGATG   2456               Increased stearate   CAAACCAGGAAAGGCAAACGAAAAGAAAAAATGGCTTTGAATTTTA   2457       stearoyl-ACP   ATGCCATCGCCTCG   T   AATCTCAGAAGCTCCCTTGCTTTGCTCTTCC       desaturase   ACCAAAGGCCACCCTTAGATCTCCCAAGT         Gossypium hirsutum     ACTTGGGAGATCTAAGGGTGGCCTTTGGTGGAAGAGCAAAGCAAG   2458       Lys11 Term   GGAGCTTCTGAGATT   A   CGAGGCGATGGCATTAAAATTCAAAGCCAA       AAA-TAA   TTTTTTCTTTTCGTTTGCCTTTCCTGGTTTG           TCGCCTCG   T   AATCTCAG   2459           CTGAGATT   A   CGAGGCGA   2460               Increased stearate   AGGAAAGGCAAACGAAAAGAAAAAATGGCTTTGAATTTTAATGCCA   2461       stearoyl-ACP   TCGCCTCGAAATCT   T   AGAAGCTCCCTTGCTTTGCTCTTCCACCAAA       desaturase   GGCCACCCTTAGATCTCCCAAGTTTTCCA         Gossypium hirsutum     TGGAAAACTTGGGAGATCTAAGGGTGGCCTTTGGTGGAAGAGCAA   2462       Gln13 Term   AGCAAGGGAGCTTCT   A   AGATTTCGAGGCGATGGCATTAAAATTCA       CAG-TAG   AAGCCATTTTTTCTTTTCGTTTGCCTTTCCT           CGAAATCT   T   AGAAGCTC   2463           GAGCTTCT   A   AGATTTCG   2464                    
     [0148]                   TABLE 24                          Oligonucleotides to produce plants with reduced linolenic acid                                 Phenotype, Gene,                   Plant &amp; Targeted       SEQ ID       Alteration   Altering Oligos   NO:               Reducing linolenic acid   AATAGAACGACAGAGACTTTTTCCTCTTTTCTTCTTGGGAAGAGGC   2465           omega-3 fatty acid   TCCAATGGCGAGCT   A   GGTTTTATCAGAATGTGGTTTTAGACCTCTC       desaturase   CCCAGATTCTACCCTAAACACACAACCTC         Arabidopsis thaliana     GAGGTTGTGTGTTTAGGGTAGAATCTGGGGAGAGGTCTAAAACCA   2466       Ser4 Term   CATTCTGATAAAACC   T   AGCTCGCCATTGGAGCCTCTTCCCAAGAAG       TCG-TAG   AAAAGAGGAAAAAGTCTCTGTCGTTCTATT           GGCGAGCT   T   GGTTTTAT   2467           ATAAAACC   A   AGCTCGCC   2468               Reducing linolenic acid   ACGACAGAGACTTTTTCCTCTTTTCTTCTTGGGAAGAGGCTCCAAT   2469       omega-3 fatty acid   GGCGAGCTCGGTTT   G   ATCAGAATGTGGTTTTAGACCTCTCCCCAG       desaturase   ATTCTACCCTAAACACACAACCTCTTTTGC         Arabidopsis thaliana     GCAAAAGAGGTTGTGTGTTTAGGGTAGAATCTGGGGAGAGGTCTA   2470       Leu6 Term   AAACCACATTCTGAT   C   AAACCGAGCTCGCCATTGGAGCCTCTTCCC       TTA-TGA   AAGAAGAAAAGAGGAAAAAGTCTCTGTCGT           CTCGGTTT   G   ATCAGAAT   2471           ATTCTGAT   C   AAACCGAG   2472               Reducing linolenic acid   ACAGAGACTTTTTCCTCTTTTCTTCTTGGGAAGAGGCTCCAATGGC   2473       omega-3 fatty acid   GAGCTCGGTTTTAT   G   AGAATGTGGTTTTAGACCTCTCCCCAGATTC       desaturase   TACCCTAAACACACAACCTCTTTTGCCTC         Arabidopsis thaliana     GAGGCAAAAGAGGTTGTGTGTTTAGGGTAGAATCTGGGGAGAGGT   2474       Ser7 Term   CTAAAACCACATTCT   C   ATAAAACCGAGCTCGCCATTGGAGCCTCTT       TCA-TGA   CCAAGAAGAAAAGAGGAAAAAGTCTCTGT           GGTTTTAT   G   AGAATGTG   2475           CACATTCT   C   ATAAAACC   2476               Reducing linolenic acid   AGAGACTTTTTCCTCTTTTCTTCTTGGGAAGAGGCTCCAATGGCGA   2477       omega-3 fatty acid   GCTCGGTTTTATCA   T   AATGTGGTTTTAGACCTCTCCCCAGATTCTA       desaturase   CCCTAAACACACAACCTCTTTTGCCTCTA         Arabidopsis thaliana     TAGAGGCAAAAGAGGTTGTGTGTTTAGGGTAGAATCTGGGGAGAG   2478       Glu8 Term   GTCTAAAACCACATT   A   TGATAAAACCGAGCTCGCCATTGGAGCCTC       GAA-TAA   TTCCCAAGAAGAAAAGAGGAAAAAGTCTCT           TTTTATCA   T   AATGTGGT   2479           ACCACATT   A   TGATAAAA   2480               Reducing linolenic acid   TCATCATCTTCTTCTTCTGGGGAGAGAGAGAGAGCAAAAGAGCTC   2481       omega-3 fatty acid   TAGCAATGGCGAACT   A   GGTCTTATCCGAATGTGGCATAAGACCTC       desaturase   TCCCCAGAATCTACACCACACCCAGATCCAC         Brassica juncea     GTGGATCTGGGTGTGGTGTAGATTCTGGGGAGAGGTCTTATGCCA   2482       Leu4 Term   CATTCGGATAAGACC   T   AGTTCGCCATTGCTAGAGCTCTTTTGCTCT       TTG-TAG   CTCTCTCTCCCCAGAAGAAGAAGATGATGA           GGCGAACT   A   GGTCTTAT   2483           ATAAGACC   T   AGTTCGCC   2484               Reducing linolenic acid   TCTTCTTCTTCTGGGGAGAGAGAGAGAGCAAAAGAGCTCTAGCAA   2485       omega-3 fatty acid   TGGCGAACTTGGTCT   G   ATCCGAATGTGGCATAAGACCTCTCCCCA       desaturase   GAATCTACACCACACCCAGATCCACTTTCCT         Brassica juncea     AGGAAAGTGGATCTGGGTGTGGTGTAGATTCTGGGGAGAGGTCTT   2486       Leu6 Term   ATGCCACATTCGGAT   C   AGACCAAGTTCGCCATTGCTAGAGCTCTTT       TTA-TGA   TGCTCTCTCTCTCTCCCCAGAAGAAGAAGA           CTTGGTCT   G   ATCCGAAT   2487           ATTCGGAT   C   AGACCAAG   2488               Reducing linolenic acid   TTCTTCTGGGGAGAGAGAGAGAGCAAAAGAGCTCTAGCAATGGCG   2489       omega-3 fatty acid   AACTTGGTCTTATCC   T   AATGTGGCATAAGACCTCTCCCCAGAATCT       desaturase   ACACCACACCCAGATCCACTTTCCTCTCCA         Brassica juncea     TGGAGAGGAAAGTGGATCTGGGTGTGGTGTAGATTCTGGGGAGA   2490       Glu8 Term   GGTCTTATGCCACATT   A   GGATAAGACCAAGTTCGCCATTGCTAGA       GAA-TAA   GCTCTTTTGCTCTCTCTCTCTCCCCAGAAGAA           TCTTATCC   T   AATGTGGC   2491           GCCACATT   A   GGATAAGA   2492               Reducing linolenic acid   CTGGGGAGAGAGAGAGAGCAAAAGAGCTCTAGCAATGGCGAACT   2493       omega-3 fatty acid   TGGTCTTATCCGAATG   A   GGCATAAGACCTCTCCCCAGAATCTACAC       desaturase   CACACCCAGATCCACTTTCCTCTCCAACACC         Brassica juncea     GGTGTTGGAGAGGAAAGTGGATCTGGGTGTGGTGTAGATTCTGG   2494       Cys9 Term   GGAGAGGTCTTATGCC   T   CATTCGGATAAGACCAAGTTCGCCATTG       TGT-TGA   CTAGAGCTCTTTTGCTCTCTCTCTCTCCCCAG           TCCGAATG   A   GGCATAAG   2495           CTTATGCC   T   CATTCGGA   2496               Reducing linolenic acid   ATAACAGAATTGCTGAATTCTTGCATTTTTAGCTTCTGGGTTTTCAA   2497       omega-3 fatty acid   TGGCTGCTGGTTG   A   GTATTATCAGAATGTGGTTTAAGGCCTCTCCC       desaturase   AAGAATCTACTCACGACCCAGAATTGGT         Ricinus communis     ACCAATTCTGGGTCGTGAGTAGATTCTTGGGAGAGGCCTTAAACC   2498       Trp5 Term   ACATTCTGATAATAC   T   CAACCAGCAGCCATTGAAAACCCAGAAGCT       TGG-TGA   AAAAATGCAAGAATTCAGCAATTCTGTTAT           GCTGGTTG   A   GTATTATC   2499           GATAATAC   T   CAACCAGC   2500               Reducing linolenic acid   AGAATTGCTGAATTCTTGCATTTTTAGCTTCTGGGTTTTCAATGGCT   2501       omega-3 fatty acid   GCTGGTTGGGTAT   G   ATCAGAATGTGGTTTAAGGCCTCTCCCAAGA       desaturase   ATCTACTCACGACCCAGAATTGGTTTTAC         Ricinus communis     GTAAAACCAATTCTGGGTCGTGAGTAGATTCTTGGGAGAGGCCTT   2502       Leu7 Term   AAACCACATTCTGAT   C   ATACCCAACCAGCAGCCATTGAAAACCCAG       TTA-TGA   AAGCTAAAAATGCAAGAATTCAGCAATTCT           TTGGGTAT   GATCAGAAT       2503           ATTCTGAT   C   ATACCCAA   2504               Reducing linolenic acid   ATTGCTGAATTCTTGCATTTTTAGCTTCTGGGTTTTCAATGGCTGCT   2505       omega-3 fatty acid   GGTTGGGTATTAT   G   AGAATGTGGTTTAAGGCCTCTCCCAAGAATCT       desaturase   ACTCACGACCCAGAATTGGTTTTACATC         Ricinus communis     GATGTAAAACCAATTCTGGGTCGTGAGTAGATTCTTGGGAGAGGC   2506       Ser8 Term   CTTAAACCACATTCT   C   ATAATACCCAACCAGCAGCCATTGAAAACC       TCA-TGA   CAGAAGCTAAAAATGCAAGAATTCAGCAAT           GGTATTAT   G   AGAATGTG   2507           CACATTCT   C   ATAATACC   2508               Reducing linolenic acid   TGCTGAATTCTTGCATTTTTAGCTTCTGGGTTTTCAATGGCTGCTG   2509       omega-3 fatty acid   GTTGGGTATTATCA   T   AATGTGGTTTAAGGCCTCTCCCAAGAATCTA       desaturase   CTCACGACCCAGAATTGGTTTTACATCGA         Ricinus communis     TCGATGTAAAACCAATTCTGGGTCGTGAGTAGATTCTTGFGGAGAG   2510       Glu9 Term   CGCCTTAAACCACATT   A   TGATAATACCCAACCAGCAGCCATTGAAAA       GAA-TAA   CCCAGAAGCTAAAAATGCAAGAATTCAGCA           TATTATCA   T   AATGTGGT   2511           ACCACATT   A   TGATAATA   2512               Reducing linolenic acid   GCAAGTTGGTTTTATCAGAATGTGGTCTTAGACCACTCCCAAGAA   2513       omega-3 fatty acid   TCTACCCTAAGCCC   T   GAACTGGGGCAGCCACTTCTGCCTCCTCTC       desaturase   ACATTAAGTTGAGAATTTCACGTACAGATC         Nicotiana tabacum     GATCTGTACGTGAAATTCTCAACTTAATGTGAGAGGAGGCAGAAGT   2514       Arg22 Term   GGCTGCCCCAGTTC   A   GGGCTTAGGGTAGFATTCTTGGGAGTGGTCT       AGA-TGA   AAGACCACATTCTGATAAAACCCAACTTGC           CTAAGCCC   T   GAACTGGG   2515           CCCAGTTC   A   GGGCTTAG   2516               Reducing linolenic acid   CTCCCAAGAATCTACCCTAAGCCCAGAACTGGGGCAGCCACTTCT   2517       omega-3 fatty acid   GCCTCCTCTCACATT   T   AGTTGAGAATTTCACGTACAGATCTGAGTG       desaturase   GTTCTGCAATTTCTTTGTCTAATACTAAT         Nicotiana tabacum     TATTAGTATTAGACAAAGAAATTGCAGAACCACTCAGATCTGTACG   2518       Lys34 Term   TGAAATTCTCAACT   A   AATGTGAGAGGAGGCAGAAGTGGCTGCCCC       AAG-TAG   AGTTCTGGGCTTAGGGTAGATTCTTGGGAG           CTCACATT   T   AGTTGAGA   2519           TCTCAACT   A   AATGTGAG   2520               Reducing linolenic acid   CAAGAATCTACCCTAAGCCCAGAACTGGGGCAGCCACTTCTGCCT   2521       omega-3 fatty acid   CCTCTCACATTAAGT   A   GAGAATTTCACGTACAGATCTGAGTGGTTC       desaturase   TGCAATTTCTTTGTCTAATACTAATAAAGA         Nicotiana tabacum     TCTTTATTAGTATTAGACAAAGAAATTGCAGAACCACTCAGATCTGT   2522       Leu35 Term   ACGTGAAATTCTC   T   ACTTAATGTGAGAGGAGGCAGAAGTGGCTGC       TTG-TAG   CCCAGTTCTGGGCTTAGGGTAGATTCTTG           CATTAAGT   A   GAGAATTT   2523           AAATTCTC   T   ACTTAATG   2524               Reducing linolenic acid   AGAATCTACCCTAAGCCCAGAACTGGGGCAGCCACTTCTGCCTCC   2525       omega-3 fatty acid   TCTCACATTAAGTTG   T   GAATTTCACGTACAGATCTGAGTGGTTCTG       desaturase   CAATTTCTTTGTCTAATACTAATAAAGAGA         Nicotiana tabacum     TCTCTTTATTAGTATTAGACAAAGAAATTGCAGAACCACTCAGATCT   2526       Arg36 Term   GTACGTGAAATTC   A   CAACTTAATGTGAGAGGAGGCAGAAGTGGCT       AGA-TGA   GCCCCAGTTCTGGGCTTAGGGTAGATTCT           TTAAGTTG   T   GAATTTCA   2527           TGAAATTC   A   CAACTTAA   2528               Reducing linolenic acid   GCGAGTTGGGTTTTATCAGAATGTGGTCTGAGGCCACTCCCGAGG   2529       omega-3 fatty acid   GTCTATCCTAAGCCA   T   GAACTGGCCACCCTTTGTTGAATTCCAATC       desaturase   CCACAAAGCTGAGATTTTCAAGAACAGATC         Sesamum indicum     GATCTGTTCTTGAAAATCTCAGCTTTGTGGGATTGGAATTCAACAA   2530       Arg22 Term   AGGGTGGCCAGTTC   A   TGGCTTAGGATAGACCCTCGGGAGTGGCC       AGA-TGA   TCAGACCACATTCTGATAAAACCCAACTCGC           CTAAGCCA   T   GAACTGGC   2531           GCCAGTTC   A   TGGCTTAG   2532               Reducing linolenic acid   CAGAATGTGGTCTGAGGCCACTCCCGAGGGTCTATCCTAAGCCAA   2533       omega-3 fatty acid   GAACTGGCCACCCTT   A   GTTGAATTCCAATCCCACAAAGCTGAGATT       desaturase   TTCAAGAACAGATCTTGGAAATGGTTCTTC         Sesamum indicum     GAAGAACCATTTCCAAGATCTGTTCTTGAAAATCTCAGCTTTGTGG   2534       Leu27 Term   GATTGGAATTCAAC   T   AAGGGTGGCCAGTTCTTGGCTTAGGATAGA       TTG-TAG   CCCTCGGGAGTGGCCTCAGACCACATTCTG           CCACCCTT   A   GTTGAATT   2535           AATTCAAC   T   AAGGGTGG   2536               Reducing linolenic acid   AATGTGGTCTGAGGCCACTCCCGAGGGTCTATCCTAAGCCAAGAA   2537       omega-3 fatty acid   CTGGCCACCCTTTGT   A   GAATTCCAATCCCACAAAGCTGAGATTTTC       desaturase   AAGAACAGATCTTGGAAATGGTTCTTCATT         Sesamum indicum     AATGAAGAACCATTTCCAAGATCTGTTCTTGAAAATCTCAGCTTTGT   2538       Leu28 Term   GGGATTGGAATTC   T   ACAAAGGGTGGCCAGTTCTTGGCTTAGGATA       TTG-TAG   GACCCTCGGGAGTGGCCTCAGACCACATT           CCCTTTGT   A   GAATTCCA   2539           TGGAATTC   T   ACAAAGGG   2540               Reducing linolenic acid   CTCCCGAGGGTCTATCCTAAGCCAAGAACTGGCCACCCTTTGTTG   2541       omega-3 fatty acid   AATTCCAATCCCACA   T   AGCTGAGATTTTCAAGAACAGATCTTGGAA       desaturase   ATGGTTCTTCATTCTGTTTGTCGAGTGGGA         Sesamum indicum     TCCCACTCGACAAACAGAATGAAGAACCATTTCCAAGATCTGTTCT   2542       Lys34 Term   TGAAAATCTCAGCT   A   TGTGGGATTGGAATTCAACAAAGGGTGGCC       AAG-TAG   AGTTCTTGGCTTAGGATAGACCCTCGGGAG           ATCCCACA   T   AGCTGAGA   2543           TCTCAGCT   A   TGTGGGAT   2544               Reducing linolenic acid   CATCAGAGCGGCGATACCTAAGCATTGCTGGGTTAAGAATCCATG   2545       omega-3 fatty acid   GAAGTCTATGAGTTA   G   GTCGTCAGAGAGCTAGCCATCGTGTTCGC       desaturase   ACTAGCTGCTGGAGCTGCTTACCTCAACAAT         Brassica napus     ATTGTTGAGGTAAGCAGCTCCAGCAGCTAGTGCGAACACGATGGC   2546       Tyr3 Term   TAGCTCTCTGACGAC   C   TAACTCATAGACTTCCATGGATTCTTAACC       TAC-TAG   CAGCAATGCTTAGGTATCGCCGCTCTGATG           ATGAGTTA   G   GTCGTCAG   2547           CTGACGAC   C   TAACTCAT   2548               Reducing linolenic acid   GCGGCGATACCTAAGCATTGCTGGGTTAAGAATCCATGGAAGTCT   2549       omega-3 fatty acid   ATGAGTTACGTCGTC   T   GAGAGCTAGCCATCGTGTTCGCACTAGCT       desaturase   GCTGGAGCTGCTTACCTCAACAATTGGCTTG         Brassica napus     CAAGCCAATTGTTGAGGTAAGCAGCTCCAGCAGCTAGTGCGAACA   2550       Arg6 Term   CGATGGCTAGCTCTC   A   GACGACGTAACTCATAGACTTCCATGGAT       AGA-TGA   CTTAACCCAGCAATGCTTAGGTATCGCCGC           ACGTCGTC   T   GAGAGCTA   2551           TAGCTCTC   A   GACGACGT   2552               Reducing linolenic acid   GCGATACCTAAGCATTGCTGGGTTAAGAATCCATGGAAGTCTATGA   2553       omega-3 fatty acid   GTTACGTCGTCAGA   T   AGCTAGCCATCGTGTTCGCACTAGCTGCTG       desaturase   GAGCTGCTTACCTCAACAATTGGCTTGTTT         Brassica napus     AAACAAGCCAATTGTTGAGGTAAGCAGCTCCAGCAGCTAGTGCGA   2554       Glu7 Term   ACACGATGGCTAGCT   A   TCTGACGACGTAACTCATAGACTTCCATG       GAG-TAG   GATTCTTAACCCAGCAATGCTTAGGTATCGC           TCGTCAGA   T   AGCTAGCC   2555           GGCTAGCT   A   TCTGACGA   2556               Reducing linolenic acid   CCATGGAAGTCTATGAGTTACGTCGTCAGAGAGCTAGCCATCGTG   2557       omega-3 fatty acid   TTCGCACTAGCTGCT   T   GAGCTGCTTACCTCAACAATTGGCTTGTTT       desaturase   GGCCTCTCTATTGGATTGCTCAAGGAACCA         Brassica napus     TGGTTCCTTGAGCAATCCAATAGAGAGGCCAAACAAGCCAATTGTT   2558       Gly17 Term   GAGGTAAGCAGCTC   A   AGCAGCTAGTGCGAACACGATGGCTAGCT       GGA-TGA   CTCTGACGACGTAACTCATAGACTTCCATGG           TAGCTGCT   T   GAGCTGCT   2559           AGCAGCTC   A   AGCAGCTA   2560               Reducing linolenic acid   GCAAGTTGGGTTCTATCAGAATGTGGTCTTAGACCACTACCAAGAA   2561       omega-3 fatty acid   TATACCCAAAGCCC   T   GAATAGGGTCTTCTTCCGTTTGCGCCACCAA       desaturase   TTTAAATCTGAGAAGAATTTCACCTTCAC         Solanum tuberosum     GTGAAGGTGAAATTCTTCTCAGATTTAAATTGGTGGCGCAAACGGA   2562       Arg22 Term   AGAAGACCCTATTC   A   GGGCTTTGGGTATATTCTTGGTAGTGGTCTA       AGA-TGA   AGACCACATTCTGATAGAACCCAACTTGC           CAAAGCCC   T   GAATAGGG   2563           CCCTATTC   A   GGGCTTTG   2564               Reducing linolenic acid   TGGTCTTAGACCACTACCAAGAATATACCCAAAGCCCAGAATAGG   2565       omega-3 fatty acid   GTCTTCTTCCGTTTG   A   GCCACCAATTTAAATCTGAGAAGAATTTCA       desaturase   CCTTCACCTATACGAACAGATCGGAATTGT         Solanum tuberosum     ACAATTCCGATCTGTTCGTATAGGTGAAGGTGAAATTCTTCTCAGA   2566       Cys29 Term   TTTAAATTGGTGGC   T   CAAACGGAAGAAGACCCTATTCTGGGCTTTG       TGC-TGA   GGTATATTCTTGGTAGTGGTCTAAGACCA           TCCGTTTG   A   GCCACCAA   2567           TTGGTGGC   T   CAAACGGA   2568               Reducing linolenic acid   CACTACCAAGAATATACCCAAAGCCCAGAATAGGGTCTTCTTCCGT   2569       omega-3 fatty acid   TTGCGCCACCAATT   G   AAATCTGAGAAGAATTTCACCTTCACCTATA       desaturase   CGAACAGATCGGAATTGTTGGGCATTGAG         Solanum tuberosum     CTCAATGCCCAACAATTCCGATCTGTTCGTATAGGTGAAGGTGAAA   2570       Leu33 Term   TTCTTCTCAGATTT   C   AATTGGTGGCGCAAACGGAAGAAGACCCTAT       TTA-TGA   TCTGGGTTTGGGTATATTCTTGGTAGTG           CACCAATT   G   AAATCTGA   2571           TCAGATTT   C   AATTGGTG   2572               Reducing linolenic acid   AGAATATACCCAAAGCCCAGAATAGGGTCTTCTTCCGTTTGCGCCA   2573       omega-3 fatty acid   CCAATTTAAATCTG   T   GAAGAATTTCACCTTCACCTATACGAACAGAT       desaturase   CGGAATTGTTGGGCATTGAGGGTAAGTG         Solanum tuberosum     CACTTACCCTCAATGCCCAACAATTCCGATCTGTTCGTATAGGTGA   2574       Arg36 Term   AGGTGAAATTCTTC   A   CAGATTTAAATTGGTGGCGCAAACGGAAGAA       AGA-TGA   GACCCTATTCTGGGCTTTGGGTATATTCT           TAAATCTG   T   GAAGAATT   2575           AATTCTTC   A   CAGATTTA   2576               Reducing linolenic acid   CTCTTTATTATCCTCCTCTTCTTTGTTTTTTTTGAGTTCTGAGTCACC   2577       omega-3 fatty acid   TATGGCAAGTTG   A   GTGATTTCAGAATGTGGGCTAAGGCCACTTCC       desaturase   AAGAATCTATGCCAGGCCCAGAAGTGGA         Petroselinum crispum     TCCACTTCTGGGCCTGGCATAGATTCTTGGAAGTGGCCTTAGCCC   2578       Trp4 Term   ACATTCTGAAATCAC   T   CAACTTGCCATAGGTGACTCAGAACTCAAA       TGG-TGA   AAAAACAAAGAAGAGGAGGATAATAAAGAG           GCAAGTTG   A   GTGATTTC   2579           GAAATCAC   T   CAACTTGC   2580               Reducing linolenic acid   TATCCTCCTCTTCTTTGTTTTTTTTGAGTTCTGAGTCACCTATGGCA   2581       omega-3 fatty acid   AGTTGGGTGATTT   G   AGAATGTGGGCTAAGGCCACTTCCAAGAATC       desaturase   TATGCCAGGCCCAGAAGTGGAGCTTCATG         Petroselinum crispum     CATGAAGCTCCACTTCTGGGCCTGGCATAGATTCTTGGAAGTGGC   2582       Ser7 Term   CTTAGCCCACATTCT   C   AAATCACCCAACTTGCCATAGGTGACTCAG       TCA-TGA   AACTCAAAAAAAACAAAGAAGAGGAGGATA           GGTGATTT   G   AGAATGTG   2583           CACATTCT   C   AAATCACC   2584               Reducing linolenic acid   TCCTCCTCTTCTTTGTTTTTTTTGAGTTCTGAGTCACCTATGGCAAG   2585       omega-3 fatty acid   TTGGGTGATTTCA   T   AATGTGGGCTAAGGCCACTTCCAAGAATCTAT       desaturase   GCCAGGCCCAGAAGTGGAGCTTCATGTT         Petroselinum crispum     AACATGAAGCTCCACTTCTGGGCCTGGCATAGATTCTTGGAAGTG   2586       Glu8 Term   GCCTTAGCCCACATT   A   TGAAATCACCCAACTTGCCATAGGTGACTC       GAA-TAA   AGAACTCAAAAAAAACAAAGAAGAGGAGGA           TGATTTCA   T   AATGTGGG   2587           CCCACATT   A   TGAAATCA   2588               Reducing linolenic acid   CTCTTCTTTGTTTTTTTTGAGTTCTGAGTCACCTATGGCAAGTTGGG   2589       omega-3 fatty acid   TGATTTCAGAAT   G   AGGGCTAAGGCCACTTCCAAGAATCTATGCCA       desaturase   GGCCCAGAAGTGGAGCTTCATGTTTCAAC         Petroselinum crispum     GTTGAAACATGAAGCTCCACTTCTGGGCCTGGCATAGATTCTTGG   2590       Cys9 Term   AAGTGGCCTTAGCCC   T   CATTCTGAAATCACCCAACTTGCCATAGGT       TGT-TGA   GACTCAGAACTCAAAAAAAACAAAGAAGAG           TCAGAATG   A   GGGCTAAG   2591           CTTAGCCC   T   CATTCTGA   2592               Reducing linolenic acid   ATGAAGCAGCAACAGTACAAAGACACCCCAATTCTAAATGGCGTTA   2593       omega-3 fatty acid   ATGGTTTTCATGCT   T   AAGAAGAAGAAGAAGAAGAGGATTTCGACTT       desaturase   AAGCAATCCTCCTCCATTCAATATTGGTC         Vernicia fordii     GACCAATATTGAATGGAGGAGGATTGCTTAAGTCGAAATCCTCTTC   2594       Lys21 Term   TTCTTCTTCTTCTT   A   AGCATGAAAACCATTAACGCCATTTAGAATTG       AAA-TAA   GGGTGTCTTTGTACTGTTGCTGCTTCAT           TTCATGCT   T   AAGAAGAA   2595           TTCTTCTT   A   AGCATGAA   2596               Reducing linolenic acid   AAGCAGCAACAGTACAAAGACACCCCAATTCTAAATGGCGTTAATG   2597       omega-3 fatty acid   GTTTTCATGCTAAA   T   AAGAAGAAGAAGAAGAGGATTTCGACTTAAG       desaturase   CAATCCTCCTCCATTCAATATTGGTCAGA         Vernicia fordii     TCTGACCAATATTGAATGGAGGAGGATTGCTTAAGTCGAAATCCTC   2598       Glu22 Term   TTCTTCTTCTTCTT   A   TTTAGCATGAAAACCATTAACGCCATTTAGAA       GAA-TAA   TTGGGGTGTCTTTGTACTGTTGCTGCTT           ATGCTAAA   T   AAGAAGAA   2599           TTCTTCTT   A   TTTAGCAT   2600               Reducing linolenic acid   CAGCAACAGTACAAAGACACCCCAATTCTAAATGGCGTTAATGGTT   2601       omega-3 fatty acid   TTCATGCTAAAGAA   T   AAGAAGAAGAAGAGGATTTCGACTTAAGCAA       desaturase   TCCTCCTCCATTCAATATTGGTCAGATCC         Vernicia fordii     GGATCTGACCAATATTGAATGGAGGAGGATTGCTTAAGTCGAAATC   2602       Glu23 Term   CTCTTCTTCTTCTT   A   TTCTTTAGCATGAAAACCATTAACGCCATTTA       GAA-TAA   GAATTGGGGTGTCTTTGTACTGTTGCTG           CTAAAGAA   T   AAGAAGAA   2603           TTCTTCTT   A   TTCTTTAG   2604               Reducing linolenic acid   CAGCAACAGTACAAAGACACCCCAATTCTAAATGGCGTTAATGGTT   2605       omega-3 fatty acid   TTCATGCTAAAGAA   T   AAGAAGAAGAAGAGGATTTCGACTTAAGCAA       desaturase   TCCTCCTCCATTCAATATTGGTCAGATCC         Vernicia fordii     GGATCTGACCAATATTGAATGGAGGAGGATTGCTTAAGTCGAAATC   2606       Glu24 Term   CTCTTCTTCTTCTT   A   TTCTTTAGCATGAAAACCATTAACGCCATTTA       GAA-TAA   GAATTGGGGTGTCTTTGTACTGTTGCTG           CTAAAGAA   T   AAGAAGAA   2607           TTCTTCTT   A   TTCTTTAG   2608               Reducing linolenic acid   GGTCCAAGCACAGCCTCTACAACATGTTGGTAATGGTGCAGGGAA   2609       omega-3 fatty acid   AGAAGATCAAGCTTA   G   TTTGATCCAAGTGCTCCACCACCCTTCAAG       desaturase   ATTGCAAATATCAGAGCAGCAATTCCAAAA         Glycine max     TTTTGGAATTGCTGCTCTGATATTTGCAATCTTGAAGGGTGGTGGA   2610       Tyr21 Term   GCACTTGGATCAAA   C   TAAGCTTGATCTTCTTTCCCTGCACCATTAC       TAT-TAG   CAACATGTTGTAGAGGCTGTGCTTGGACC           CAAGCTTA   G   TTTGATCC   2611           GGATCAAA   C   TAAGCCTG   2612               Reducing linolenic acid   GGTAATGGTGCAGGGAAAGAAGATCAAGCTTATTTTGATCCAAGT   2613       omega-3 fatty acid   GCTCCACCACCCTTC   T   AGATTGCAAATATCAGAGCAGCAATTCCAA       desaturase   AACATTGCTGGGAGAAGAACACATTGAGAT         Glycine max     ATCTCAATGTGTTCTTCTCCCAGCAATGTTTTGGAATTGCTGCTCT   2614       Lys31 Term   GATATTTGCAATCT   A   GAAGGGTGGTGGAGCACTTGGATCAAAATAA       AAG-TAG   GCTTGATCTTCTTTCCCTGCACCATTACC           CACCCTTC   T   AGATTGCA   2615           TGCAATCT   A   GAAGGGTG   2616               Reducing linolenic acid   AAAGAAGATCAAGCTTATTTTGATCCAAGTGCTCCACCACCCTTCA   2617       omega-3 fatty acid   AGATTGCAAATATC   T   GAGCAGCAATTCCAAAACATTGCTGGGAGAA       desaturase   GAACACATTGAGATCTCTGAGTTATGTTC         Glycine max     GAACATAACTCAGAGATCTCAATGTGTTCTTCTCCCAGCAATGTTTT   2618       Arg36 Term   GGAATTGCTGCTC   A   GATATTTGCAATCTTGAAGGGTGGTGGAGCA       AGA-TGA   CTTGGATCAAAATAAGCTTGATCTTCTTT           CAAATATC   T   GAGCAGCA   2619           TGCTGCTC   A   GATATTTG   2620               Reducing linolenic acid   TATTTTGATCCAAGTGCTCCACCACCCTTCAAGATTGCAAATATCA   2621       omega-3 fatty acid   GAGCAGCAATTCCA   T   AACATTGCTGGGAGAAGAACACATTGAGAT       desaturase   CTCTGAGTTATGTTCTGAGGGATGTGTTGG         Glycine max     CCAACACATCCCTCAGAACATAACTCAGAGATCTCAATGTGTTCTT   2622       Leu41 Term   CTCCCAGCAATGTT   A   TGGAATTGCTGCTCTGATATTTGCAATCTTG       AAA-TAA   AAGGGTGGTGGAGCACTTGGATCAAAATA           CAATTCCA   T   AACATTGC   2623           GCAATGTT   A   TGGAATTG   2624               Reducing linolenic acid   CATCCACCCGCACCCGCACCCGCCCCGCTGACGGCGGCAATGGC   2625       omega-3 fatty acid   CCGGCTCGTGCTCTCC   T   AGTGCTCGGGCCTCGCGCCCGTCCGCC       desaturase   GCCTGCGCGCCGGCCGGGGCGCCATTGCGGCGC         Zea mays     GCGCCGCAATGGCGCCCCGGCCGGCGCGCAGGCGGCGGACGG   2626       Glu8 Term   GCGCGAGGCCCGAGCACT   A   GGAGAGCACGAGCCGGGCCATTGC       GAG-TAG   CGCCGTCAGCGGGGCGGGTGCGGGTGCGGGTGGATG           TGCTCTCC   T   AGTGCTCG   2627           CGAGCACT   A   GGAGAGCA   2628               Reducing linolenic acid   ACCCGCACCCGCACCCGCCCCGCTGACGGCGGCAATGGCCCGG   2629       omega-3 fatty acid   CTCGTGCTCTCCGAGTG   A   TCGGGCCTCGCGCCCGTCCGCCGCCT       desaturase   GCGCGCCGGCCGGGGCGCCATTGCGGCGCGGTCA         Zea mays     TGACCGCGCCGCAATGGCGCCCCGGCCGGCGCGCAGGCGGCGG   2630       Cys9 Term   ACGGGCGCGAGGCCCGA   T   CACTCGGAGAGCACGAGCCGGGCCA       TGC-TGA   TTGCCGCCGTCAGCGGGGCGGGTGCGGGTGCGGGT           TCCGAGTG   A   TCGGGCCT   2631           AGGCCCGA   T   CACTCGGA   2632               Reducing linolenic acid   CCGCACCCGCACCCGCCCCGCTGACGGCGGCAATGGCCCGGCT   2633       omega-3 fatty acid   CGTGCTCTCCGAGTGCT   A   GGGCCTCGCGCCCGTCCGCCGCCTGC       desaturase   GCGCCGGCCGGGGCGCCATTGCGGCGCGGTCACC         Zea mays     GGTGACCGCGCCGCAATGGCGCCCCGGCCGGCGCGCAGGCGGC   2634       Ser10 Term   GGACGGGCGCGAGGCCC   T   AGCACTCGGAGAGCACGAGCCGGGC       TCG-TAG   CATTGCCGCCGTCAGCGGGGCGGGTGCGGGTGCGG           CGAGTGCT   A   GGGCCTCG   2635           CGAGGCCC   T   AGCACTCG   2636               Reducing linolenic acid   GCTCGGGCCTCGCGCCCGTCCGCCGCCTGCGCGCCGGCCGGGG   2637       omega-3 fatty acid   CGCCATTGCGGCGCGGT   G   ACCCCCCGCGCTCTCCGCGGCGCCG       desaturase   CGCCGTCGTCCCGCGTCCGCGTCCATCCACCGCGA         Zea mays     TCGCGGTGGATGGACGCGGACGCGGGACGACGGCGCGGCGCCG   2638       Ser29 Term   CGGAGAGCGCGGGGGGT   C   ACCGCGCCGCAATGGCGCCCCGGCC       TCA-TGA   GGCGCGCAGGCGGCGGACGGGCGCGAGGCCCGAGC           GGCGCGGT   G   ACCCCCCG   2639           CGGGGGGT   C   ACCGCGCC   2640               Reducing linolenic acid   CCCCCTCCCCCACGCACACGCACAGATCCATCCGCGGCCATGGC   2641       omega-3 fatty acid   CCCCGCAATGAGGCCG   T   AGCAGGAGGCGAGCTGCAAGGCCACCG       desaturase   AGGACCACCGCTCCGAGTTCGACGCCGCCAAGC         Triticum aestivum     GCTTGGCGGCGTCGAACTCGGAGCGGTGGTCCTCGGTGGCCTTG   2642       Glu8 Term   CAGCTCGCCTCCTGCT   A   CGGCCTCATTGCGGGGGCCATGGCCGC       GAG-TAG   GGATGGATCTGTGCGTGTGCGTGGGGGAGGGGG           TGAGGCCG   T   AGCAGGAG   2643           CTCCTGCT   A   CGGCCTCA   2644               Reducing linolenic acid   CCTCCCCCACGCACACGCACAGATCCATCCGCGGCCATGGCCCC   2645       omega-3 fatty acid   CGCAATGAGGCCGGAG   T   AGGAGGCGAGCTGCAAGGCCACCGAG       desaturase   GACCACCGCTCCGAGTTCGACGCCGCCAAGCCGC         Triticum aestivum     GCGGCTTGGCGGCGTCGAACTCGGAGCGGTGGTCCTCGGTGGCC   2646       Gln9 Term   TTGCAGCTCGCCTCCT   A   CTCCGGCCTCATTGCGGGGGCCATGGC       CAG-TAG   CGCGGATGGATCTGTGCGTGTGCGTGGGGGAGG           GGCCGGAG   T   AGGAGGCG   2647           CGCCTCCT   A   CTCCGGCC   2648               Reducing linolenic acid   CCCCCACGCACACGCACAGATCCATCCGCGGCCATGGCCCCCGC   2649       omega-3 fatty acid   AATGAGGCCGGAGCAG   T   AGGCGAGCTGCAAGGCCACCGAGGACC       desaturase   ACCGCTCCGAGTTCGACGCCGCCAAGCCGCCGC         Triticum aestivum     GCGGCGGCTTGGCGGCGTCGAACTCGGAGCGGTGGTCCTCGGT   2650       Glu10 Term   GGCCTTGCAGCTCGCCT   A   CTGCTCCGGCCTCATTGCGGGGGCCA       GAG-TAG   TGGCCGCGGATGGATCTGTGCGTGTGCGTGGGGG           CGGAGCAG   T   AGGCGAGC   2651           GCTCGCCT   A   CTGCTCCG   2652               Reducing linolenic acid   ACGCACAGATCCATCCGCGGCCATGGCCCCCGCAATGAGGCCGG   2653       omega-3 fatty acid   AGCAGGAGGCGAGCTG   A   AAGGCCACCGAGGACCACCGCTCCGA       desaturase   GTTCGACGCCGCCAAGCCGCCGCCCTTCCGCATC         Triticum aestivum     GATGCGGAAGGGCGGCGGCTTGGCGGCGTCGAACTCGGAGCGG   2654       Cys13 TermTGGTCCTCGGTGGCCTT   T   CAGCTCGCCTCCTGCTCCGGCCTCATT       TGC-TGA   GCGGGGGCCATGGCCGCGGATGGATCTGTGCGT           GCGAGCTG   A   AAGGCCAC   2655           GTGGCCTT   T   CAGCTCGC   2656               Reducing linolenic acid   CTTCACAAATCACAAATCGGAATCAGATCCACCACGACACCCCGG   2657       omega-3 fatty acid   CGGCAATGGCGGCGT   A   GGCGACCCAGGAGGCCGACTGCAAGGC       desaturase   TTCCGAGGACGCCCGTCTCTTCTTCGACGCCGC         Oryza sativa     GCGGCGTCGAAGAAGAGACGGGCGTCCTCGGAAGCCTTGCAGTC   2658       Ser4 Term   GGCCTCCTGGGTCGCC   T   ACGCCGCCATTGCCGCCGGGGTGTCGT       TCG-TAG   GGTGGATCTGATTCCGATTTGTGATTTGTGAAG           GGCGGCGT   A   GGCGACCC   2659           GGGTCGCC   T   ACGCCGCC   2660               Reducing linolenic acid   ATCACAAATCGGAATCAGATCCACCACGACACCCCGGCGGCAATG   2661       omega-3 fatty acid   GCGGCGTCGGCGACC   T   AGGAGGCCGACTGCAAGGCTTCCGAGGA       desaturase   CGCCCGTCTCTTCTTCGACGCCGCCAAGCCCC         Oryza sativa     GGGGCTTGGCGGCGTCGAAGAAGAGACGGGCGTCCTCGGAAGC   2662       Gln7 Term   CTTGCAGTCGGCCTCCT   A   GGTCGCCGACGCCGCCATTGCCGCCG       CAG-TAG   GGGTGTCGTGGTGGATCTGATTCCGATTTGTGAT           CGGCGACC   T   AGGAGGCC   2663           GGCCTCCT   A   GGTCGCCG   2664               Reducing linolenic acid   ACAAATCGGAATCAGATCCACCACGACACCCCGGCGGCAATGGC   2665       omega-3 fatty acid   GGCGTCGGCGACCCAG   T   AGGCCGACTGCAAGGCTTCCGAGGACG       desaturase   CCCGTCTCTTCTTCGACGCCGCCAAGCCCCCGC         Oryza sativa     GCGGGGGCTTGGCGGCGTCGAAGAAGAGACGGGCGTCCTCGGA   2666       Glu8 Term   AGCCTTGCAGTCGGCCT   A   CTGGGTCGCCGACGCCGCCATTGCCG       GAG-TAG   CCGGGGTGTCGTGGTGGATCTGATTCCGATTTGT           CGACCCAG   T   AGGCCGAC   2667           GTCGGCCT   A   CTGGGTCG   2668               Reducing linolenic acid   TCAGATCCACCACGACACCCCGGCGGCAATGGCGGCGTCGGCGA   2669       omega-3 fatty acid   CCCAGGAGGCCGACTG   A   AAGGCTTCCGAGGACGCCCGTCTCTTC       desaturase   TTCGACGCCGCCAAGCCCCCGCCCTTCCGCATC         Oryza sativa     GATGCGGAAGGGCGGGGGCTTGGCGGCGTCGAAGAAGAGACGG   2670       Cys10 Term   GCGTCCTCGGAAGCCTT   T   CAGTCGGCCTCCTGGGTCGCCGACGC       TGC-TGA   CGCCATTGCCGCCGGGGTGTCGTGGTGGATCTGA           GCCGACTG   A   AAGGCTTC   2671