Patent Publication Number: US-11644476-B2

Title: Analysis device

Description:
CROSS-REFERENCE TO RELATED APPLICATION 
     This application is based on and claims priority under 35 USC 119 from Japanese Patent Application No. 2017-209709 filed on Oct. 30, 2017, the entire contents of which is incorporated by reference herein. 
     TECHNICAL FIELD 
     The present application relates to an analysis device. 
     BACKGROUND ART 
     An analysis device for analyzing a component present in a sample is described in Japanese Patent Application Laid-Open (JP-A) No. 2012-093350, where a device in which component analysis is performed by inducing electrophoresis in the sample in a capillary within in a chip, and measuring transmitted light or reflected light from light illuminated onto the sample. 
     In cases in which an analysis kit employed in such an analysis device is an analysis kit including the chip and a cartridge that has been filled in advance which liquid to be supplied to the chip, it is desirable for the chip and the cartridge to be fitted together reliably without positional misalignment therebetween. 
     SUMMARY 
     The present application is directed to an analysis kit provided with a chip and a cartridge in which the chip and the cartridge are fitted together reliably. 
     One aspect of the present application is an analysis device into which is placed an analysis kit including a chip provided with a capillary through which a sample flows and a cartridge superimposed on the chip and provided with a liquid reservoir, and in which a component present in sample can be measured in a state in which the chip and the cartridge have been fitted together. The analysis device includes a placement section, a pressing member, and a measurement member. The analysis kit is placed on the placement section. 
     The pressing member presses the analysis kit placed on the placement section in a direction in which the cartridge is superimposed on the chip to sandwich the analysis kit between the pressing member and the placement section, and to fit the chip and the cartridge together. The measurement member measures the component present in the sample in the analysis kit in which the chip and the cartridge have been fitted together. 
     The aspects enable the chip and the cartridge to be fitted together reliably in an analysis kit provided with the chip and the cartridge. 
    
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         FIG.  1    is a perspective view illustrating external configuration of an analysis device of a first exemplary embodiment. 
         FIG.  2    is a perspective view illustrating an analysis kit employed for analysis in an analysis device. 
         FIG.  3    is an exploded perspective view of the analysis kit illustrated in  FIG.  2   . 
         FIG.  4    is a cross-section of the analysis kit, taken along line  4 - 4  in  FIG.  2   . 
         FIG.  5    is a front view illustrating a guide-in section and the vicinity thereof inside the analysis device of the first exemplary embodiment. 
         FIG.  6    is a plan view illustrating a guide-in section and the vicinity thereof inside the analysis device of the first exemplary embodiment. 
         FIG.  7    is a front view illustrating a guide-in section and the vicinity thereof inside the analysis device of the first exemplary embodiment. 
         FIG.  8    is a plan view illustrating a guide-in section and the vicinity thereof inside the analysis device of the first exemplary embodiment. 
         FIG.  9    is a perspective view illustrating a guide-in section and the vicinity thereof inside the analysis device of the first exemplary embodiment. 
         FIG.  10    is a perspective view illustrating a pressing member of the analysis device of the first exemplary embodiment. 
         FIG.  11    is a cross-section illustrating a state in which a sealing film has been pierced by a piercing pin in the analysis device of the first exemplary embodiment. 
         FIG.  12    is a front view illustrating a guide-in section and the vicinity thereof inside the analysis device of the first exemplary embodiment, and illustrating a state in which an analysis kit is tilted. 
         FIG.  13    is a side view illustrating a guide-in section and the vicinity thereof inside the analysis device of the first exemplary embodiment, and illustrating a state in which an analysis kit is tilted. 
         FIG.  14    is a front view illustrating a state in which an illumination member has been inserted into an analysis kit inside the analysis device of the first exemplary embodiment. 
         FIG.  15    is a front view illustrating a state in which an illumination member has been inserted into an analysis kit inside the analysis device of the first exemplary embodiment. 
         FIG.  16    is a front view illustrating a state in which a pressing member has pressed an analysis kit inside the analysis device of the first exemplary embodiment. 
         FIG.  17    is a diagram illustrating an analysis kit in a state partway through analysis of a sample by an analysis device. 
         FIG.  18    is a diagram illustrating an analysis kit in a state partway through analysis of a sample by an analysis device. 
         FIG.  19    is a diagram illustrating an analysis kit in a state partway through analysis of a sample by an analysis device. 
         FIG.  20    is a front view illustrating a state in which a power supply probe has been inserted into an analysis kit inside the analysis device of the first exemplary embodiment. 
         FIG.  21    is a plan view illustrating a state in which a power supply probe has been inserted into an analysis kit inside the analysis device of the first exemplary embodiment. 
         FIG.  22    is a diagram illustrating an analysis kit in a state partway through analysis of a sample by an analysis device. 
         FIG.  23    is a cross-section illustrating a state in which a sealing film has been pierced by a piercing pin in an analysis device of a second exemplary embodiment. 
         FIG.  24    is a cross-section illustrating a state in which a sealing film has been pierced by a piercing pin in an analysis device of a third exemplary embodiment. 
         FIG.  25    is a cross-section illustrating a state in which a sealing film has been pierced by a piercing pin in an analysis device of a fourth exemplary embodiment. 
         FIG.  26    is a cross-section illustrating a state in which a sealing film has been pierced by a piercing pin in an analysis device of a reference example. 
     
    
    
     DESCRIPTION OF EMBODIMENTS 
     First Exemplary Embodiment 
     Explanation follows regarding an analysis device of a first exemplary embodiment, with reference to the drawings. The analysis device of the first exemplary embodiment is, for example, a device used to analyze the amount of glycated hemoglobin present in blood. Blood is one example of a sample, which is also referred to as a “specimen.” Glycated hemoglobin is one example of an analysis target of the analysis device. 
     External Configuration of the Analysis Device 
     As illustrated in  FIG.  1   , an analysis device  102  includes a casing  104 . In the first exemplary embodiment, the casing  104  is formed in a substantially rectangular box shape. In the following explanation, a vertical direction, a width direction, and a depth direction of the analysis device  102  are respectively indicated by an arrow U, an arrow W, and an arrow D. The arrow W direction, the arrow D direction, and directions with both arrow W direction and arrow D direction components are all horizontal directions. The far side and near side in the depth direction of the analysis device  102  are respectively indicated by an arrow DA and an arrow DB. 
     The casing  104  is provided with a touch panel not illustrated in the drawings. A technician performing an analysis task is able to operate the analysis device  102  by touching the touch panel while referring to information displayed on the touch panel. 
     The casing  104  is also provided with a printer not illustrated in the drawings. The analysis device  102  is capable of printing analysis results for a sample using the printer. 
     A near face  108  of the casing  104  is provided with an opening/closing cover  114 . The opening/closing cover  114  is capable of sliding between a projecting position illustrated by double-dotted dashed lines, in which the opening/closing cover  114  has been moved toward the near side by an opening/closing mechanism  116 , and a loaded position illustrated by a solid line in which the opening/closing cover  114  has been moved toward the far side so as to lie in the same plane as the near face  108 . When the opening/closing cover  114  is in the projecting position, a tray  118  and the opening/closing cover  114  are exposed at the near side of the casing  104 . An analysis kit  42  containing a specimen or sample can be placed on this tray. 
     Analysis Kit Configuration 
     As illustrated in  FIG.  2    to  FIG.  4   , as an example, the analysis kit  42  of the first exemplary embodiment has a structure including a chip  44  and a cartridge  46 . The analysis kit  42  is set on the tray  118  of the analysis device  102  in a state in which the cartridge  46  is superimposed over the chip  44 , with the arrow D 1  side on the far side. In the analysis kit  42 , for convenience, the vertical direction, width direction, and depth direction in a state in which the analysis device  102  has been set in the tray  118  are labeled with the arrow U, arrow W, and arrow D respectively. The far side and the near side of the analysis kit  42  are labeled with the arrow D 1  and the arrow D 2  respectively. 
     The chip  44  is formed by sticking together two plates, an upper plate  44 A and a lower plate  44 B, that have the same external profile as each other in plan view as viewed along the arrow A 1  direction. In a state in which the plates  44 A,  44 B have been stuck to each other, the chip  44  configures a plate shaped member. When the chip  44  is lying in a horizontal state, the plate thickness direction of the chip  44  is aligned with a vertical direction. The cartridge  46  is superimposed on the chip  44  in the vertical direction, namely in the plate thickness direction of the chip  44 . 
     The chip  44  is formed with plural channels  48 . 
     The cross-section profile of the channels  48 , and the layout of the channels  48  in plan view of the chip  44 , are not limited. The channels  48  may bend at one or more locations, or may be straight. 
     The flow path cross-section area of the channels  48  is set such that when pressure is applied to a liquid by a pump  172 , described later with respect to  FIG.  11   , the pressurized liquid flows through the channels  48 . 
     A protrusion  50  that projects toward the cartridge  46  is formed at an end portion of each channel  48 . As described later, the protrusion  50  is an example of a piercing projection that pierces a bottom-face film  58 . 
     The cartridge  46  is formed with plural liquid reservoirs  52 . 
     Each liquid reservoir  52  is a recess formed in an upper portion of the cartridge  46 . Upper faces of the liquid reservoirs  52  are sealed off by a sealing film  54 . In the first exemplary embodiment, as illustrated in  FIG.  4   , the plural liquid reservoirs  52  are sealed off by a single sealing film  54 . However, individual sealing films  54  may be provided for each liquid reservoir  52 . It is sufficient that the material of the sealing film  54  seals the liquid sealed in the liquid reservoirs  52  to prevent evaporation of the liquid, and that the sealing film  54  is pierced by a piercing member provided to the analysis device, described later. One example of such a material is a laminate film with a multi-layered structure, with one or more of the multi-layered structure including, but not limited to, PET. 
     Communication portions  56  that place the liquid reservoirs  52  in communication with the channels of the chip  44  are formed in a lower portion of each of the liquid reservoirs  52 . Liquid LA, such as a diluent or a migration liquid, is encapsulated in some of the plural liquid reservoirs  52 . Lower portions of the communication portions  56  of the liquid reservoirs  52  encapsulating the liquid are each sealed off using the bottom-face film  58 . 
     Note that a filter may be provided upstream of a capillary  68 , described later, in the flow of the liquid in the channels  48  of the chip  44 . Such a filter enables a structure to be achieved in which foreign matter other than the liquid is removed and does not flow into the capillary  68 . 
     The capillary  68  is formed between channels  48  that each correspond to two specific communication portions  56  of two specific liquid reservoirs  52  from out of the plural liquid reservoirs  52 . The flow path cross-section area of the capillary  68  is set such that liquid present in the channels  48  flows through the capillary  68  due to capillary action. Accordingly, the flow path cross-section area of the capillary  68  is smaller than the flow path cross-section area of any of the channels  48 . Electrodes  62  are provided to the communication portions  56  on either side of the capillary  68 . 
     As illustrated in  FIG.  21   , one side face  46 A of the cartridge  46  is formed with side-face holes  64  corresponding to the respective electrodes  62 . The side-face holes  64  are holes that reach from one of two side faces of the analysis kit  42 , such as the one side face  46 A, to the corresponding electrode  62 . As described later, a power supply probe  194 , which is an example of a power supply member, of the analysis device  102  is inserted into each side-face hole  64  in the cartridge  46  and placed in contact with the electrode  62 , enabling a voltage to be applied between the two electrodes  62 . 
     Note that the one side face  46 A and the other side face  46 B of the cartridge  46  are the same faces as one side face  42 A and the other side face  42 B of the analysis kit  42 . 
     In the first exemplary embodiment, the capillary  68  has a structure in which a groove formed in the lower plate  44 B is covered by the upper plate  44 A. Accordingly, in effect, the capillary  68  is formed in the lower plate  44 B of the chip  44 . 
     An insertion hole  70  is formed in the analysis kit  42  (the cartridge  46  and the chip  44 ) from an upper face side, at a location corresponding to an intermediate position of the capillary  68 . In the first exemplary embodiment, part of the insertion hole  70  is also formed in the upper plate  44 A of the chip  44 . 
     As shown in  FIG.  4   , an illumination member  176  of the analysis device  102  is inserted into the insertion hole  70 . The illumination member  176  is a member that illuminates a sample for electrophoresis in the capillary  68  with light from an illumination portion  176 A at the tip of the illumination member  176 . The illumination portion  176 A of the illumination member  176  contacts a bottom  70 B of the insertion hole  70 . 
     As illustrated in  FIG.  3   , the chip  44  is formed with a notch  72  on the same side as the other side face  42 B of the analysis kit  42 . The notch  72  has an isosceles triangle shaped profile in plan view. The notch  72  is an example of a recess  71 . The notch  72  includes two oblique faces  72 A,  72 B that are oblique with respect to a guide-in direction, as represented by arrow D 1  direction toward the far side, of the analysis kit  42 . A positioning pin  140 A, described later, fits into the notch  72 . The oblique faces  72 A,  72 B contact the positioning pin  140 A. 
     The chip  44  is also formed with a notch  73 , serving as a recess  71 , at a separate position to that of the notch  72 . Unlike the notch  72 , the notch  73  has a substantially trapezoidal profile or rectangular profile in plan view. A positioning pin  140 B, described later, fits into the notch  72 . A back face  71 D contacts the positioning pin  140 B. 
     As an alternative configuration to that of the notch  72  and the notch  73  described above, three or more notches may be provided as recesses  71 . Moreover, the profiles of the recesses  71  are not limited to the profiles of the notch  72  or notch  73 . The profiles thereof are not limited as long as they contact or fit together with contact members such as positioning pins of the analysis device in a combination capable of positioning the analysis kit. 
     Note that the other side face  46 B of the cartridge  46  is formed with an escape portion  46 C to avoid contact with the positioning pins  140 A,  140 B. See  FIG.  9   . 
     In the analysis kit  42 , the cartridge  46  is installed above the chip  44 . In this state, claws  74  formed to the cartridge  46  engage with the chip  44 , thereby integrating the chip  44  and the cartridge  46  into a single unit. In this integrated state, the cartridge  46  and the chip  44  can be fitted together by moving the chip  44  and the cartridge  46  relatively toward one another. When the chip  44  and the cartridge  46  are in both the integrated state and the fitted-together state, the external profile of the analysis kit  42  is a substantially rectangular block shape. The fitted-together state of the chip  44  and the cartridge  46  enables analysis of a component in the electrophoresing sample inside the capillary  68  to be performed. 
     An operation to fit the chip  44  and the cartridge  46  together may be performed by an analysis technician, or may be performed in the analysis device  102 , as described later. In the fitted-together state, the bottom-face films  58  are pierced by the respective protrusions  50  located at positions corresponding to the bottom-face films  58 . The protrusions  50  are an example of piercing projections that pierce the bottom-face films  58  configuring bottom faces of the liquid reservoirs  52 . However, the piercing projection may have any profile as long as it is a profile capable of breaking the seal of the bottom-face film  58 . 
     An example has been given in which the analysis kit  42  is configured by the chip  44  and the cartridge  46 . However, any profile may be employed as long as it is a profile configured such that one side face is pushed in by a pusher member  128 , described later, provided to the analysis device  102 , and after being pushed in by the pusher member  128 , the profile contacts a contact member at the other side face on the opposite side to the one side face. For example, there is no limitation to a rectangular block shape, and an oval column shape, or a circular column shape configured with a stepped profile on a given side face, may be employed. Likewise, although the configuration may include a capillary provided inside the analysis kit  42  into which the migration liquid and the sample are introduced with electrophoresis being induced in the sample, any configuration of analysis kit may be employed that includes a sample capable of being introduced into the analysis device  102  of the present application, positioned, and measured. For example, there is no limitation to an analysis kit employed with an electrophoresis method, and other examples of analysis kits include an analysis kit employed in an electrochemical measurement method, a colorimetric measurement method, an immunological measurement method, or the like. Application may be made to any analysis kit for which positioning of the analysis kit in a measurement apparatus is demanded. 
     Internal Configuration of the Analysis Device 
     As illustrated in  FIG.  5    to  FIG.  8   , a guide-in section is provided inside the casing  104  of the analysis device  102  shown in  FIG.  1    at position where the tray  118  is loaded. The tray  118  retracts or moves toward the far side into the casing  104  such that an analysis kit  42  placed on the tray  118  is guided into the guide-in section. The guide-in section is a location into which the analysis kit  42 , provided with the electrodes  62  to induce electrophoresis in the sample in the capillary  68 , is guided. 
     The guide-in section includes a placement section  122 , a pressing member  124  disposed above the placement section  122 , and a measurement member  126 . As described later, the measurement member  126  measures a component contained in the sample in the analysis kit  42  in a state in which the analysis kit  42  has been guided into the guide-in section. More specifically, in the first exemplary embodiment, the measurement member  126  measures a component contained in the sample using a light shone onto the sample flowing through the capillary  68  of the analysis kit  42 . As an example, as illustrated in  FIG.  4   , the measurement member  126  includes an optical absorbance sensor  186  and a measurement section  190  that identifies the type and amount of a component present in the sample based on data from the optical absorbance sensor  186 . 
     The placement section  122  includes a main portion  122 A at a predetermined height as viewed along the depth direction, represented by the arrow D direction, of the analysis device  102 , and a placement mount  122 B that projects upward at a width direction central portion of the main portion  122 A. The analysis kit  42  is placed on the placement mount  122 B of the placement section  122 . An upper face  42 T and the channels  48  of the analysis kit  42  are horizontal in a state in which the analysis kit  42  has been placed on the placement mount  122 B. 
     As illustrated in  FIG.  5    and  FIG.  7   , the guide-in section is provided with the pusher member  128 . 
     A pusher rod  134  is retained in the pusher member  128  by a retention mechanism, not illustrated in the drawings. The pusher rod  134  is capable of approaching and retreating from the one side face  46 A of the analysis kit  42 . A leading end portion of the pusher rod  134  configures a pusher portion  134 P that contacts and pushes the one side face  46 A of the analysis kit  42  inward. 
     A pusher spring not illustrated in the drawings is installed to the pusher rod  134 . A pusher motor not illustrated in the drawings pushes the pusher rod  134  in the arrow P 1  direction through the pusher spring, and the pusher portion  134 P at the leading end of the pusher rod  134  contacts the one side face  46 A of the analysis kit  42 . In this state, the pusher rod  134  continues moving in the direction toward the analysis kit  42 , thus pushing the analysis kit  42  in the arrow P 1  direction. Note that as described later, the chip  44  of the analysis kit  42  contacts the positioning pins  140 A,  140 B, and the pusher spring compresses in a state in which movement of the analysis kit  42  in the arrow P 1  direction is obstructed. This suppresses the pusher rod  134  from being pushing the analysis kit  42  too far. The specific configuration of the pusher member is not limited to that described above, and any configuration may be employed as long as the configuration is capable of contacting the one side face  46 A of the analysis kit  42  so as to push the analysis kit  42  in the arrow P 1  direction. 
     One or plural positioning pins are provided for the analysis kit  42 , so as to project upright at the side of the chip  44  formed with the notch  72  (the other side face  42 B side), namely at the opposite side of the chip  44  to the pusher rod  134 . For example, in the first exemplary embodiment, the two positioning pins  140 A,  140 B are provided with a gap between each other in the depth direction. The positioning pins  140 A,  140 B are examples of protrusions, and are also examples of contact members. Moreover, the pusher member  128  and the contact members, such as positioning pins  140 A,  140 B, configure together an example of a positioning member that positions the analysis kit  42  at a predetermined position. 
     As illustrated in  FIG.  9   , both the positioning pins  140 A,  140 B are formed in circular column shapes with conical leading end portions or upper end portions. Moreover, the heights of the positioning pins  140 A,  140 B reach as high as the lower plate  44 B of the analysis kit  42  when placed on the placement mount  122 B, but do not reach as high as the upper plate  44 A. The upper plate  44 A is formed smaller than the lower plate  44 B in the vicinity of the position of the positioning pins  140 A,  140 B, such that the positioning pins  140 A,  140 B would not contact the upper plate  44 A even if they were to be formed higher due to dimensional tolerances. 
     The positioning pin  140 A on the near side is located at a depth direction, represented by arrow D direction, position corresponding to where the notch  72  is formed on the chip  44 . The positioning pin  140 B on the far side is located at a depth direction, represented by arrow D direction, position where the recess  71  is formed, further toward the far side than the notch  72 . 
     As illustrated in  FIG.  6   , the width direction, represented by arrow W direction, positions of the positioning pins  140 A,  140 B are positions that do not contact the analysis kit  42  in a state in which the analysis kit  42  has simply been guided into the guide-in section. However, when the analysis kit  42  is pushed in the arrow P 1  direction by the pusher rod  134 , the lower plate  44 B of the chip  44  contacts the positioning pins  140 A,  140 B, thereby positioning the analysis kit  42  in the width direction. 
     Note that when either of the two oblique faces  72 A,  72 B of the notch  72  makes contact with the positioning pin  140 A, the analysis kit  42  also moves in the depth direction. Thus, as illustrated in  FIG.  8   , both the oblique faces  72 A,  72 B are thereby positioned at positions contacting the positioning pin  140 A. 
     The configuration of the contact members and protrusions is not limited to that described above, and any configuration may be employed in which contact is made with the side of the analysis kit  42  formed with the recesses  71 , i.e. the other side face  42 B side, when the analysis kit  42  is pushed and moved in the arrow P 1  direction, so as to position the analysis kit  42  in the width direction. In particular, contact members and protrusions may be configured with any profile that corresponds to the profile of the analysis kit, and the contact members are preferably configured by protrusions. Configuring the contact members as protrusions enables a structure to be achieved in which the analysis kit reliably contacts the protruding portions or projecting portions. Contact members configured by protrusions are more preferably pins that project from the placement section on which the analysis kit is placed. The positioning pins  140 A,  140 B are an example of this configuration. The protrusions can be configured by a simple structure in a structure in which the protrusions are pins. 
     As also illustrated in  FIG.  10   , the pressing member  124  is provided above the placement section  122 . The pressing member  124  includes an opposing wall  142  that opposes the upper face of the analysis kit  42  when the analysis kit  42  has been guided into the guide-in section. The opposing wall  142  is moved vertically by a vertical drive mechanism  144 . 
     In the first exemplary embodiment, the vertical drive mechanism  144  includes an elevator motor  148 , driven under the control of a controller  146 . Drive force of the elevator motor  148  acts on the opposing wall  142  through a spring, not illustrated in the drawings, so as to raise and lower the opposing wall  142 . 
     Driving the elevator motor  148  lowers the opposing wall  142  via the spring, such that the opposing wall  142  contacts the upper face  42 T of the analysis kit  42 . This is a structure in which the non-illustrated spring would compress were driving of the elevator motor  148  to be continued after making contact, and the opposing wall  142  would not descend any further. A structure is thereby achieved in which the opposing wall  142  does not press the analysis kit  42  excessively. 
     The opposing wall  142  includes a wall body  160  that has a predetermined rigidity, and a close contact sheet  162  affixed to a lower face of the wall body  160 . The close contact sheet  162  has a lower modulus of elasticity than both the wall body  160  and the cartridge  46  of the analysis kit  42 , and therefore elastically deforms readily when applied with external force. 
     Namely, in a state in which the opposing wall  142  has been pushed in toward the analysis kit  42 , the close contact sheet  162  is elastically compressed in its thickness direction (the vertical direction) to a greater degree than the wall body  160  and the cartridge  46 . Accordingly, as illustrated in  FIG.  16   , when the opposing wall  142  descends, the close contact sheet  162  makes close contact with the upper face  42 T of the analysis kit  42 . The opposing wall  142  is also an example of a close contact portion. 
     In particular, the analysis kit  42  of the first exemplary embodiment includes the plural liquid reservoirs  52 . The single close contact sheet  162  corresponding to the plural liquid reservoirs  52  makes close contact with the upper face  42 T of the analysis kit  42 . The analysis kit  42  can accordingly be pressed and gripped between the opposing wall  142  and the placement section  122  in the height direction of the analysis kit  42 , namely the direction in which the chip  44  and the cartridge  46  are superimposed on each other. 
     The wall body  160  is provided with plural piercing pins  164 , corresponding to each of the respective liquid reservoirs  52 . The piercing pins  164  are an example of piercing members. Each of the piercing pins  164  projects downward further than the close contact sheet  162 . The piercing pins  164  are capable of piercing the sealing film  54  at the corresponding liquid reservoirs  52  when the opposing wall  142  descends. 
     As illustrated in  FIG.  11   , in a state in which the analysis kit  42  has been pressed by the pressing member  124  and the close contact sheet  162  has made close contact with the upper face  42 T of the analysis kit  42 , lower ends of the respective piercing pins  164  are positioned further toward the lower side than the sealing film  54 , namely within the corresponding liquid reservoirs  52 . Gaps GP are formed between holes HP formed by the piercing pins  164  and the piercing pins  164 . 
     As illustrated in  FIG.  10   , for example, bent portions  165  are formed at the leading ends of piercing pins  164  corresponding to the liquid reservoirs  52  in which liquid has been encapsulated in advance out of the plural liquid reservoirs  52 . For example, these are the piercing pins  164 A,  164 E in the example of  FIG.  10   ; however, there is no limitation thereto. At the bent portions  165 , the piercing pins  164  are bent in a direction intersecting the pressing direction of the pressing member  124 , i.e. the downward direction. For example, the piercing pins  164  having the bent portions  165  are intersecting at approximately 90° in the example of  FIG.  10   . The piercing pins  164  formed with the bent portions  165  are able to pierce a larger hole in the sealing film  54  than piercing pins  164  not formed with the bent portions  165 , resulting in a larger gap GP formed between the hole HP and the piercing pin  164 . 
     Spacing recesses  166  indented toward the upper side are formed on the opposing wall  142  (the close contact sheet  162  and the wall body  160 ) with profiles that surround the piercing pins  164 . Namely, the opposing wall  142 , this being an example of a close contact portion, is formed with the spacing recesses  166 . The spacing recesses  166  are portions where the opposing wall  142  is locally indented in a direction away from the liquid reservoirs  52  at the periphery of the piercing pins  164 . Due to the presence of the spacing recesses  166 , airtight spaces  168  are formed between the respective liquid reservoirs  52  and the opposing wall  142 . This configuration, in which the spacing recesses  166  of the analysis device  102  and the upper face of the analysis kit  42  are utilized to form the airtight spaces  168  between the periphery of locations pierced by the piercing members and the analysis kit  42 , results in an airtight member. Of course, the profile of the airtight spaces and method for forming the airtight spaces, as well as the profile of the airtight member, may be modified as appropriate in accordance with the profiles of the analysis device  102  and the analysis kit  42 . 
     The opposing wall  142  is provided with gas introduction tubes  170 , serving as an example of gas introduction members, corresponding to the respective spacing recesses  166 . A lower end of each gas introduction tube  170  configures a gas port  170 A through which a fluid enters and leaves the airtight spaces  168 . In the first exemplary embodiment, the position of the lower end of each of the plural gas introduction tubes  170 , namely the position of each of the gas ports  170 A, is the same position as that of an upper face  166 T of the corresponding spacing recess  166 . A structure is thereby configured in which the gas port  170 A of the gas introduction tube  170  is positioned at the airtight space  168  but does not project into the corresponding spacing recess  166 . 
     The pump  172  is connected to the gas introduction tubes  170 . Driving the pump  172  enables air to be fed into the airtight spaces  168 , or to be sucked out of the airtight spaces  168 . Note that a single pump  172  may be provided with a branching configuration so as to be common to the plural gas introduction tubes  170 . In such cases, the structure may be configured with valves, not illustrated in the drawings, to switch the air flow path to a desired gas introduction tube  170 . 
     As illustrated in  FIG.  11   , the gas introduction tubes  170  and the piercing pins  164  are offset from each other in the horizontal direction, which is at least one direction out of the depth direction or the width direction. Namely, the gas ports  170 A of the gas introduction tubes  170  are at positions offset from the locations, i.e., the holes HP, in the film surface of the sealing film  54  pierced by the piercing pins  164 . 
     The piercing pins  164  described above are one example of a piercing member. The piercing members may have any profile capable of piercing the sealing film  54  at the upper face of the liquid reservoirs  52  in the analysis kit  42 . The gas introduction tubes  170  described above are one example of a gas introduction member, which may have any profile capable of introducing gas to the airtight spaces. Note that in the first exemplary embodiment, the piercing members are separate members to the gas introduction members. Both have profiles capable of suppressing liquid from the liquid reservoirs  52  from adhering to the gas introduction members through which gas enters the liquid reservoirs  52  of the analysis kit  42 , and profiles capable of suppressing liquid from the liquid reservoirs  52  from flowing into the gas ports. 
     As illustrated in  FIG.  10   , the opposing wall  142  is formed with a through hole  174 . An illumination member  176  is inserted through the through hole  174 . Light emitted from a light emitting section, not illustrated in the drawings, is guided to the illumination member  176  through an optical fiber or the like. The illumination member  176  is a member that illuminates this light through an illumination portion  176 A at a leading end thereof. In effect, the illumination member  176  may configure part of the optical fiber. Note that an LED chip that emits light in a predetermined wavelength region, an optical filter, a lens, and the like may be provided, and the light emitting section may have a profile provided with a slit or the like. 
     The illumination member  176  is inserted through the through hole  174 , thereby suppressing horizontal direction misalignment of the illumination member  176  with respect to the opposing wall  142 . 
     The position of the through hole  174  is a position corresponding to the insertion hole  70  in the analysis kit  42  when positioned at the predetermined position in the guide-in section. The external diameter of the illumination member  176  is smaller than the internal diameter of the through hole  174 . This achieves a structure in which the illumination member  176  can be inserted into the insertion hole  70 . A lower side portion of the illumination member  176  configures a projection  176 T that projects to the lower side of the close contact sheet  162 . 
     A limiting plate  178  that is broader than the through hole  174  is attached to an upper portion of the illumination member  176 , namely on the opposite side of the illumination member  176  to the side projecting from the pressing member  124 . The limiting plate  178  is an example of a limiting member that limits the projection amount of the projection  176 T toward the analysis kit to within a specific range. The limiting plate  178  may have any profile as long as it has a limiting function. One or plural support columns  180  project upward from the opposing wall  142 , the support columns  180  penetrating the limiting plate  178 . 
     A leading end of each support column  180  is formed with an opposing plate  182  that is broader than the support column  180 . The limiting plate  178  is positioned between the opposing wall  142  and the opposing plates  182 . The limiting plate  178  is capable of moving vertically while being guided by the support columns  180 , with the range of this vertical movement limited to between the opposing wall  142  and the opposing plate  182 . This thereby enables a projection range of the projection  176 T, being the projection length from the opposing wall  142 , to be limited to within a predetermined range, thus enabling the projection  176 T to be suppressed from projecting excessively from the opposing wall  142 . The opposing wall  142  and the opposing plates  182  form a pair so as to oppose the limiting plate  178  from both sides in an approach/retreat direction of the projection  176 T. Namely, the opposing wall  142  and the opposing plates  182  are an example of a pair of opposing members. The opposing wall  142  also doubles as a portion of an opposing member. Accordingly, the opposing wall  142  is configured from fewer components than in a structure in which an opposing member is configured by a separate member. 
     A spring  184  is interposed between the limiting plate  178  and each opposing plate  182 . Biasing force of the springs  184  biases the illumination member  176  downward via the limiting plate  178 . The springs  184  that apply this biasing force are an example of biasing members that bias the projection  176 T in its direction of projection from the pressing member  124 . Any profile that achieves such a biasing function may be adopted for the biasing member. Accordingly, the projection  176 T of the illumination member  176  can be maintained in a state projecting by a predetermined projection amount from the opposing wall  142 . The range of downward movement of the projection  176 T is limited to a predetermined range due to the limiting plate  178  contacting the opposing wall  142 , as illustrated in  FIG.  14    and  FIG.  15   . 
     As illustrated in  FIG.  14   , in a state in which the limiting plate  178  is in contact with the opposing wall  142 , a maximum value of a projection length TL of the projection  176 T is longer than a depth SD of the insertion hole  70 . 
     Accordingly, when the opposing wall  142  descends and the illumination member  176  is inserted into the insertion hole  70 , the illumination portion  176 A at the leading end of the illumination member  176  contacts the bottom  70 B of the insertion hole  70 . In this manner, the illumination member  176  and the limiting plate  178  descend while maintaining a constant positional relationship with the opposing wall  142  until the illumination portion  176 A contacts the bottom  70 B. 
     If the opposing wall  142  attempts to move further downward when in this state, due to the presence of a gap between the limiting plate  178  and the opposing plates  182 , the illumination member  176  does not move downward even though the opposing wall  142  does move downward (while compressing the springs  184 ) so as to reduce this gap. See  FIG.  16   . 
     In the first exemplary embodiment, the direction in which the analysis kit  42  is pressed by the pressing member  124  (the direction in which the opposing wall  142  approaches the analysis kit  42 ), is the same direction as the direction in which the illumination member  176  approaches the analysis kit  42 . A movement trajectory of the pressing member  124  partially coincides with a movement trajectory of the illumination member, enabling these members to be disposed within a smaller amount of space than in a structure in which these movement trajectories are entirely discrete from one another. 
     The optical absorbance sensor  186  is provided to the guide-in section at a position below the insertion hole  70  when the analysis kit  42  has been set at the predetermined position. Light from the illumination member  176  is illuminated onto the electrophoresing sample in the capillary  68 , and the measurement member  126  takes a measurement based on light transmitted through the sample. For example, the optical absorbance sensor  186  detects optical absorbance based on this transmitted light. As another example, a measurement member may be provided with a photodiode, a photo-IC, or the like. 
     As illustrated in  FIG.  10   , plural tilt detection rods  188 , serving as an example of a tilt detection section, are attached to the opposing wall  142 . The tilt detection section is a member that detects tilt of the analysis kit  42  with respect to the horizontal direction, specifically, whether or not such tilt is present, when the analysis kit  42  has been guided into the guide-in section. Such tilt can be detected both in cases in which tilt is present in the width direction, as illustrated in  FIG.  12   , and in cases in which tilt is present in the depth direction, as illustrated in  FIG.  13   . Each of the tilt detection rods  188  is retained so as to be capable of moving vertically with respect to the opposing wall  142 . 
     As illustrated in  FIG.  5    and  FIG.  7   , the position of a lower end of each tilt detection rod  188  is a position that does not contact the analysis kit  42  in a state in which the analysis kit  42  has been introduced to the guide-in section and is not tilted. However, as illustrated in  FIG.  12    and  FIG.  13   , the positions of the lower ends of the tilt detection rods  188  are set to predetermined positions such that the lower ends of one or more of the tilt detection rods  188  contact the analysis kit  42  in cases in which the analysis kit  42  is tilted. When the opposing wall  142  descends further in a state in which a tilt detection rod  188  has contacted the analysis kit  42 , the tilt detection rod  188  moves upward relative to the opposing wall  142 . When the controller  146  detects such upward movement of the tilt detection rod  188 , the controller  146  determines the analysis kit  42  to be tilted and is able to perform predetermined processing. 
     As illustrated in  FIG.  5    to  FIG.  8   , the power supply probes  194  project into the guide-in section at locations corresponding to the plural side-face holes  64  in the analysis kit  42 . Each power supply probe  194  is driven by a motor, not illustrated in the drawings, controlled by the controller  146 , so as to approach or retreat from the one side face  46 A of the analysis kit  42 . 
     The power supply probes  194  are an example of a power supply member. Each of the plural power supply probes  194  approaches the one side face  46 A of the analysis kit  42  and is inserted into the corresponding side-face hole  64  so as to contact the respective electrode  62 . 
     The respective power supply probes  194  are positioned on one side of the analysis kit  42  that has been retained at the predetermined position in the guide-in section, but are not present anywhere other than this side. Accordingly, a structure is achieved in which various members can be disposed at positions that avoid the power supply probes  194 . For example, the pressing member  124  is disposed above the analysis kit  42 , thereby suppressing interference between the pressing member  124  and the power supply probes  194 . Moreover, the placement section  122  is disposed below the analysis kit  42 , thereby suppressing interference between the power supply probes  194  and the placement section  122 . Note that the profile of the power supply member is not particularly limited as long as power can be supplied to the electrodes  62 . 
     Next, explanation follows regarding operation of the analysis device  102  of the first exemplary embodiment, and a method for analyzing a component contained in a sample in the analysis kit  42 . 
     First, some of the channels  48  of the analysis kit  42  are filled with a sample, such as blood in the first exemplary embodiment. 
     A predetermined input operation is performed using the touch panel of the analysis device  102  so as to move the opening/closing cover  114  toward the near side and to expose the tray  118 , as illustrated by the double-dotted dashed lines in  FIG.  1   . 
     In a state in which an analysis kit  42  containing a sample has been placed on the tray  118 , the tray  118  and the opening/closing cover  114  are pushed (or a predetermined operation is performed using the non-illustrated touch panel of the analysis device  102 ) so as to move the tray  118  toward the far side. Accordingly, as illustrated in  FIG.  5   , the analysis kit  42  is guided into the guide-in section. The analysis kit  42  is placed on the placement mount  122 B of the placement section  122 . 
     The analysis device  102  includes the tilt detection section. In this state, the analysis device  102  detects any tilt of the analysis kit  42  using the tilt detection section. Specifically, the controller  146  drives the elevator motor  148  to cause the opposing wall  142  of the pressing member  124  to descend to a predetermined position. 
     When this is performed, in cases in which the analysis kit  42  is tilted as illustrated in  FIG.  12    or  FIG.  13   , one or more of the plural tilt detection rods  188  contact the analysis kit  42 . In such cases, the controller  146  halts driving of the elevator motor  148 , and performs predetermined processing in response to the fact that the analysis kit  42  is tilted. The “predetermined processing” referred to here includes, for example, processing to temporarily halt component analysis from being performed on the sample, and to notify a technician that the analysis kit  42  is tilted. 
     Providing the plural tilt detection rods  188  enables the tilt detection precision to be suppressed from deteriorating according to the tilt direction or the tilt amount (tilt angle) of the analysis kit  42 . 
     The structure of the tilt detection section used to detect tilt of the analysis kit  42  is not limited to that described above. For example, a structure may be employed in which light is illuminated onto the analysis kit  42  from plural locations in order to detect tilt. 
     In cases in which the analysis kit  42  is not tilted, including cases in which any tilt is within a permissible range, the controller  146  performs positioning of the analysis kit  42  by executing positioning in a positioning direction. Note that the state when positioning is performed is, as illustrated in  FIG.  6   , a state in which the notch  72  of the analysis kit  42  and the positioning pin  140 A are not in contact with each other, and the recess  71  and the positioning pin  140 B are not in contact with each other. 
     The controller  146  drives the pusher motor, not illustrated in the drawings, such that the pusher rod  134  pushes the one side face  46 A of the analysis kit  42 . Accordingly, as illustrated in  FIG.  7    and  FIG.  8   , the chip  44  of the analysis kit  42  contacts the positioning pins  140 A,  140 B. This thereby positions the analysis kit  42  at the predetermined position. 
     In particular, in the first exemplary embodiment, the height of the positioning pin  140 A reaches the height of the lower plate  44 B of the chip  44 , but does not reach the height of the upper plate  44 A. The lower plate  44 B of the chip  44  contacts the positioning pins  140 A,  140 B, thereby positioning the analysis kit  42 . Due to the capillary  68  being formed in the lower plate  44 B of the chip  44 , this in effect enables accurate positioning of the capillary  68 . 
     The chip  44  is formed with the notch  72 . When the analysis kit  42  approaches the positioning pin  140 A, either the oblique face  72 A or the oblique face  72 B contacts the positioning pin  140 A, and as the analysis kit  42  is pushed further in, the analysis kit  42  is also moved in the depth direction. As illustrated in  FIG.  8   , the analysis kit  42  is thus positioned at a position where both the oblique face  72 A and the oblique face  72 B contact the positioning pin  140 A. Namely, the analysis kit  42  is positioned not only in the width direction but also in the depth direction, which is the direction of guiding into the guide-in section  120 . 
     In this state, the positioning pin  140 A fits together with the notch  72 , and the positioning pin  140 B fits together with the notch  73 , thereby suppressing positional misalignment in the depth direction when the analysis kit  42  is in a positioned state. 
     Next, the controller  146  drives the elevator motor  148  to cause the opposing wall  142  to descend. Accordingly, as illustrated in  FIG.  14   , the illumination member  176  also descends so as to approach the analysis kit  42 . The analysis kit  42  has already been positioned at the predetermined position, thereby positioning the insertion hole  70 . The illumination member  176  is thus inserted into the insertion hole  70  without contacting the upper face  42 T of the analysis kit  42 . 
     Then, as illustrated in  FIG.  15   , the illumination portion  176 A at the lower end of the illumination member  176  contacts the bottom  70 B of the insertion hole  70 . Even if the elevator motor  148  continues to be driven in this state, as illustrated in  FIG.  16   , the illumination member  176  and the limiting plate  178  do not descend any further, thereby enabling the illumination portion  176 A of the illumination member  176  to be suppressed from sustaining damage as a result of being pushed hard against the bottom  70 B. 
     When the opposing wall  142  descends, the plural piercing pins  164  pierce the sealing film  54  at the corresponding liquid reservoirs  52 . The close contact sheet  162  then makes close contact with the upper face  42 T of the analysis kit  42 . 
     In this state, as illustrated in  FIG.  11   , the airtight spaces  168  are formed at each of the plural liquid reservoirs  52  between the periphery of the locations pierced by the piercing pins  164  and the analysis kit  42 . The close contact sheet  162  makes close contact in a state of face-against-face contact with the upper face  42 T of the analysis kit  42 , thereby enabling the airtight state of the airtight spaces  168  to be more reliably maintained than, for example, in a structure in which a close contact member makes line contact therewith. 
     Moreover, in this state, the analysis kit  42  is sandwiched from above and below between the pressing member  124  and the placement section  122  on which the analysis kit  42  has been placed, such that the cartridge  46  and the chip  44  are fitted together. Moreover, as illustrated in  FIG.  17   , since the bottom-face films  58  corresponding to the respective protrusions  50  are pierced, liquid is capable of flowing out downward from the liquid reservoirs  52  where the bottom-face film  58  has been pierced. Note that the cartridge  46  and the chip  44  make close contact in the vertical direction, thereby suppressing the formation of gaps between the cartridge  46  and the chip  44  where liquid would leak out from the liquid reservoirs  52  where the bottom-face film  58  has been pierced. 
     The analysis kit  42  is pressed by the pressing member  124  and retained between the placement section  122  and the pressing member  124 , thereby suppressing positional misalignment of the analysis kit  42 . In the first exemplary embodiment, since the analysis kit  42  is positioned by positioning members, the analysis kit  42  can be retained in a positioned state by pressing the analysis kit  42  with the pressing member  124 . 
     Moreover, even in a structure in which a large amount of force is required to fit the cartridge  46  and the chip  44  together, the cartridge  46  and the chip  44  can be reliably fitted together since the analysis kit  42  is sandwiched and pressed between the placement section  122  and the pressing member  124 . Moreover, the analysis kit  42  can be pressed by the pressing member  124  while maintaining an inserted state of the illumination member  176  into the insertion hole  70 . 
     Note that the controller  146  drives the pump  172 , such that gas is supplied into or sucked out from a specific liquid reservoir  52  at a predetermined timing. See  FIG.  11   . The gaps GP are formed between the piercing pins  164  and the sealing film  54  at the locations pierced by the piercing pins  164 , permitting the movement of air passing through the gaps GP between the airtight spaces  168  and the corresponding liquid reservoirs  52 . 
     As an example, first, as illustrated in  FIG.  17   , air is introduced or forced into one of the liquid reservoirs  52 , such as the liquid reservoir  52  on the left side in  FIG.  17   . The sample is thereby diluted and agitated by the liquid LA, and fed to another liquid reservoir  52  through a specific channel  48 . 
     Then, as illustrated in  FIG.  18   , air is, for example, introduced or forced into a different liquid reservoir  52 , such as the liquid reservoir  52  on the right side in  FIG.  18   . The liquid LA in this liquid reservoir  52  thus fills the channel  48  connected to this liquid reservoir  52 . Then, as illustrated in  FIG.  19   , the liquid fills the capillary  68  as a result of capillary action. 
     The piercing pins  164  that pierce the sealing film  54  are separate members to the gas introduction tubes  170  that introduce gas or fluid to the airtight spaces  168 . Moreover, the gas ports  170 A at the lower ends of the gas introduction tubes  170  are positioned within the corresponding airtight spaces  168 . Accordingly, the liquid in the liquid reservoirs  52  is suppressed from entering the interior of the gas introduction tubes  170 . For example, diluted sample from a previous sample analysis is suppressed from remaining inside the gas introduction tubes  170 , thereby suppressing a situation from arising in which such remaining diluted sample mixes with a diluted sample from the current analysis. 
     Moreover, in the first exemplary embodiment, the gas ports  170 A are positioned offset from the locations of the film surface of the sealing film  54  that are pierced by the piercing pins  164 . Accordingly, the liquid in the liquid reservoirs  52  can be suppressed from flowing into the gas ports  170 A even if the liquid were to pass through the gap between a piercing pin  164  and the sealing film  54  and enter the airtight space  168 . 
     Moreover, since the gas ports  170 A are separated from the pierced locations, even were a fragment of a member configuring the sealing film  54  to break off when the sealing film  54  is pierced by the piercing pins  164 , such a fragment can be suppressed from entering a gas port  170 A. 
     Next, the controller  146  drives the pusher motor, not illustrated in the drawings, such that the power supply probes  194  approach the one side face  46 A of the analysis kit  42 , as illustrated in  FIG.  20    and  FIG.  21   . The power supply probes  194  are inserted into the corresponding side-face holes  64 , and contact the electrodes  62 . At this stage, the analysis kit  42  has already been positioned in the depth direction. Namely, the analysis kit  42  has already been positioned in a direction intersecting the direction in which the power supply probes  194  approach the analysis kit  42 , a direction orthogonal thereto in the example of  FIG.  21   . Accordingly, the leading ends of the power supply probes  194  are reliably inserted into the side-face holes  64 , without touching the one side face  46 A of the analysis kit  42 . 
     In this state, the controller  146  applies a predetermined voltage between the electrodes  62  via the power supply probes  194 , as illustrated in  FIG.  22   . This induces electrophoresis in the component present in the sample in the capillary  68 . Moreover, when this is performed, the controller  146  causes light to be illuminated from the illumination portion  176 A of the illumination member  176 . The optical absorbance of the electrophoresing diluted sample is detected by the optical absorbance sensor  186  in order to measure the component present in the sample. 
     The illumination portion  176 A of the illumination member  176  is in contact with the bottom  70 B of the insertion hole  70  when this is being performed. Namely, the illumination portion  176 A is at a position a short distance from the capillary  68 , and this distance is kept constant. This thereby enables light to be illuminated onto the electrophoresing sample within the capillary  68  in a stable manner. 
     Moreover, since the analysis kit  42  is sandwiched between the opposing wall  142  of the pressing member  124  and the placement section  122  so as to be retained at the predetermined position, the position of the analysis kit  42  is stable. Since the capillary  68  is formed in the chip  44  of the analysis kit  42 , the position of the capillary  68  is also stable. This thereby enables analysis of the component present in the sample to be performed more accurately. 
     After completion of analysis of the component present in the sample, the controller  146  withdraws the power supply probes  194  from the side-face holes  64 , and raises the opposing wall  142 , such that the analysis device  102  is no longer pressed, and moreover the illumination member  176  is removed from the insertion hole  70 . Moreover, the pusher rod  134  is retracted and separated from the analysis device  102 . 
     The opening/closing cover  114  and the tray  118  are then moved toward the near side, permitting removal of the analysis kit  42 . The analysis kit  42  is disposable once analysis has been completed. 
     In the first exemplary embodiment, the analysis kit  42  is packaged with the measurement target sample and the liquids necessary for measurement, such as a diluent LA and a migration liquid LA, are housed therein. There is therefore no need to set up the analysis device  102  in advance with liquids required for measurement, and there is no need for a storage section to temporarily store such liquids, nor for a pump or the like to feed these liquids to the measurement site. This thereby enables the analysis device  102  to be simplified in structure and made more compact. 
     In the above explanation, the rod-shaped positioning pins  140 A,  140 B are given as an example of contact members; however, there is no limitation to such rod-shaped pins. For example, contact members may be configured by plate shaped members. Employing rod-shaped pins as the contact members enables the contact members to be disposed in a smaller space than would be possible in the case of plate shaped members. Providing plural of the rod-shaped pins enables the analysis kit  42  contacted by the pins to be better suppressed from rotating than in a configuration in which only a single rod-shaped pin is provided, thereby enabling stable positioning. 
     Second to Fourth Exemplary Embodiments, Reference Example 
     Explanation follows regarding a second to a fourth exemplary embodiment, and a reference example. In the following exemplary embodiments and in the reference example, the overall configuration of the analysis device is similar to that of the first exemplary embodiment, and detailed explanation thereof is therefore omitted. Elements, members, and so on similar to those of the first exemplary embodiment are allocated the same reference numerals, and detailed explanation thereof is omitted. 
     In an analysis device  202  of a second exemplary embodiment, illustrated in  FIG.  23   , the lower end portions of the gas introduction tubes  170  are configured by projections  170 B projecting further downward than upper faces  166 T of spacing recesses  166 . The lower end portions of the gas introduction tubes  170  also configure leading end portions thereof that contain the gas ports  170 A. 
     In the second exemplary embodiment, the lower end portion of each gas introduction tube  170  projects out. Accordingly, even were liquid that had flowed into an airtight space  168  to move toward the gas introduction tube  170  along the upper face  166 T, the liquid would be blocked by the gas introduction tube  170  itself, thereby enabling the liquid to be suppressed from flowing into the gas port  170 A. 
     In an analysis device  302  of a third exemplary embodiment, illustrated in  FIG.  24   , a wall member  196  is formed extending downward from the upper face  166 T of each spacing recess  166 . The wall member  196  is positioned between the piercing pin  164  and the gas introduction tube  170 . The position of a lower end of the wall member  196  is a position that does not contact the sealing film  54 , such that a gap is formed between the lower end of the wall member  196  and the sealing film  54 . 
     In the third exemplary embodiment, due to the presence of such a wall member  196 , even were liquid that had flowed into an airtight space  168  to move toward the gas introduction tube  170  along the upper face  166 T, the liquid would be blocked by the wall member  196 . This thereby enables the liquid to be suppressed from flowing into the gas port  170 A. The profile of the wall member  196  is not limited, as long as the wall member  196  is positioned between a piercing member, for example, the piercing pin  164 , and a gas introduction member, for example, the gas introduction tube  170 . Namely, the movement of liquid along the upper face  166 T toward the gas introduction tube  170  inside the airtight space  168  can be blocked regardless of the profile of the wall member. 
     An analysis device  402  of a fourth exemplary embodiment, illustrated in  FIG.  25   , has a structure in which a structure of the second exemplary embodiment is combined with a structure of the third exemplary embodiment in which formation of the wall members  196 . The configuration in which the projections  170 B are provided at the lower end portions of the gas introduction tubes  170  is shown in  FIG.  25   . Accordingly, in the fourth exemplary embodiment, liquid that has flowed into an airtight space  168  can be even more reliably suppressed from flowing into the gas port  170 A. 
       FIG.  26    illustrates part of an analysis device  502  of the reference example. In the structure illustrated in  FIG.  26   , the gas introduction tubes  170  perform a dual function as the piercing pins  164 , enabling the sealing film  54  to be pierced by the leading ends of the gas introduction tubes  170 . Moreover, the gas ports  170 A are formed in side faces of the gas introduction tubes  170  so as to be positioned above the sealing film  54 , namely within the airtight spaces  168 . 
     The gas ports  170 A are not positioned in the liquid reservoirs  52  even in the structure of the reference example. This enables liquid from the liquid reservoirs  52  to be suppressed from flowing into the gas ports  170 A. 
     In the first aspect, the pressing member presses the analysis kit placed on the placement section to sandwich the analysis kit between the pressing member and the placement section. This thereby enables the chip of the analysis kit and the cartridge superimposed on the chip to be fitted together reliably. Moreover, in a fitted-together state of the chip and the cartridge, the component present in the sample in the analysis kit can be measured by the measurement member. 
     A second aspect is the first aspect, wherein a piercing projection to pierce a bottom-face film configuring a bottom face of the liquid reservoir is provided to the chip in the analysis kit. Moreover, pressing the pressing member against the analysis kit causes the chip and the cartridge to approach each other such that the bottom-face film is pierced by the piercing projection. 
     The pressing member presses the analysis kit, causing the chip and the cartridge to approach each other, such that the bottom-face film of the liquid reservoir in the cartridge is ruptured by the piercing projection of the chip. This thereby enables the bottom-face film to be pierced by the simple operation of pressing the analysis kit with the pressing member. 
     A third aspect is either the first aspect or the second aspect, further including a piercing member to pierce a sealing film configuring an upper face of the liquid reservoir. Moreover, pressing by the pressing member causes the sealing film to be pierced by the piercing member. 
     The sealing film configuring the upper face of the liquid reservoir can be pierced by the piercing member by the simple operation of pressing the analysis kit with the pressing member. 
     A fourth aspect is the third aspect, wherein the pressing member includes an airtight member and a gas introduction member. The airtight member forms an airtight space against the liquid reservoir at the periphery of a location pierced by the piercing member, and the gas introduction member introduces gas into the airtight space. 
     The airtight member forms the airtight space at the periphery of the pierced location. When the gas introduction member introduces gas, for example air, into the airtight space, the gas flows into the liquid reservoir through a through hole at the pierced location of the sealing film. This thereby enables the pressure of the liquid reservoir to be increased or reduced. 
     A fifth aspect is the fourth aspect, wherein the airtight member makes face-to-face contact with the analysis kit. 
     This enables an airtight state of the airtight space to be reliably maintained, enabling air to be suppressed from moving between the interior and the exterior of the airtight space. 
     A sixth aspect is the fifth aspect, wherein the airtight member is formed from a material that has a lower modulus of elasticity than that of the analysis kit. 
     The airtight member is elastically compressed so as to make close contact with the analysis kit, enabling a close contact state to be reliably maintained, and enabling damage to the analysis kit to be suppressed. 
     A seventh aspect is any one of the third aspect to the sixth aspect, further including a bent portion that is provided at a leading end side of the piercing member and that is bent in a direction intersecting a direction in which the analysis kit is pressed. 
     The bent portion at the leading end of the piercing member is bent in a direction intersecting the direction in which the analysis kit is pressed. This thereby enables a hole with a larger opening cross-sectional area to be formed in the sealing film than when employing a piercing member with a structure lacking the bent portion.