Patent Publication Number: US-5523209-A

Title: Methods for identifying inhibitors of integrin activation

Description:
This invention was supported in part by the U.S. Government under grant numbers HL48728, HL28235, and AR27214 awarded by the National Institute of Health. The Government has certain rights in the invention. 
    
    
     BACKGROUND OF THE INVENTION 
     Cells alter their adhesiveness in response to developmental events and environmental cues. These adaptations are often mediated through integrins, adhesion receptors composed of two transmembrane subunits, α and β (Hynes, Cell 69:11-25, 1992). Rapid changes in integrin function are critical in cell migration, cellular aggregation, and leukocyte transmigration during inflammation (Hynes, Cell 69:11-25, 1992; Albelda and Buck, FASEB 4:2868-2880, 1990; Hemler, Annu. Rev. Immunol. 8:365-400, 1990; Dustin et al., J. Immunol. 148:2654-2663, 1992; Springer, Nature 346:425-434, 1990; Ginsberg et al., Curr. Opin. Cell Biol. 4:766-771, 1992; Ruoslahti, J. Clin. Invest. 87:1-5, 1991). A given integrin may also manifest varying adhesive competence depending on its cellular environment (Chan and Hemler, J. Cell. Biol. 120:537-543, 1993; Masumoto and Hemler, J. Biol. Chem. 268:228-234, 1993; Weitzman et al., J. Biolo Chem. 268:8651-8657, 1993; Elices and Hemler, Proc. Natl. Acad. Sci. USA 86:9906-9910, 1989; Kirchofer et al., J. Biol. Chem. 265:18525-18530, 1990), or the state of differentiation of the cell in which it is expressed (Haimovich et al., Cell Regulation 2:271-283, 1991; Neugebauer and Reichardt, Nature 350:68-71, 1991; Adams and Watt, Cell 63:425-435, 1990; Chan and Hemler, J. Cell Biol. 120:537-543, 1993). Such variations in function may be due to changes in ligand binding affinity as occurs with certain β 3  (Bennett and Vilaire, J. Clin. Invest. 64:1393-1401, 1979), β 2  (Altieri et al., J. Cell Biol. 107:1893-1900, 1988), and β 1  (Faull et al., J. Cell Biol. 121:155-162, 1993) integrins. Changes in adhesive function may also occur without changes in ligand binding affinity. For example, phorbol esters stimulate the α 5  β 1  -dependent adhesion of Chinese Hamster ovary cells (Danilov and Juliano, J. Cell. Biol. 108:1925-1933, 1989) to fibronectin (Fn) with no change in Fn binding affinity. Similarly, certain β 3  mutations reduce α IIb  β 3  -dependent cell adhesion to fibrinogen (Fg) without changing Fg binding affinity (Ylanne et al., J. Cell Biol. 122:223-233, 1993). Such affinity-independent changes in integrin function are ascribed to &#34;post receptor occupancy events&#34; (Danilov and Juliano, J. Cell. Biol. 108:1925-1933, 1989). Nevertheless, the host cell governs the capacity of solubilized recombinant α 2  β 1  to bind to collagen sepharose (Chan and Hemler, J. Cell. Biol. 120:537-543, 1993). This last result suggests that some cell type-specific differences in integrin function may be due to differences in ligand binding affinity. 
     A variety of in vitro treatments may alter integrin affinity. When purified α IIb  β 3  is pretreated with RGD peptides, it subsequently binds Fg and PACl (Duet al., Cell 65:409-416, 1991; Smyth et al., J. Biol. Chem. 267:15568-15577, 1992). Certain anti-β 3  antibodies directly increase the Fg binding affinity of α IIb  β 3  (Frelinger et al., J. Biol. Chem. 266:17106-17111, 1991) and certain anti-β 1  antibodies activate α 5  β 1  to bind Fn with high affinity (Faull et al., J. Cell Biolo 121:155-162, 1993). Changes in the divalent cation composition of the extracellular medium, proteolytic digestion, and treatment with reducing agents may also &#34;activate&#34; integrins (Kirchofer et al., J. Biol. Chem. 265:18525-18530, 1990; Gailit and Ruoslahti, J. Biol. Chem. 263:12927-12932, 1988; Altieri, J. Immunol. 147:1891-1898, 1991; Masumoto and Hemler, J. Biol. Chem. 268:228-234, 1993; Weitzman et al., J. Biol. Chem. 268:8651-8657, 1993; Zucker and Nachmias, Arteriosclerosis 5:2-18, 1985; Grant and Zucker, Proc. Soc. Exp. Biol. Med. 165:114-117, 1980). Thus, moieties that interact with the extracellular domain can modulate integrin affinity. Furthermore, lipid environment can alter the ligand binding capacity of an integrin (Smyth et al., J. Biol. Chem. 267:15568-15577, 1992; Conforti et al., J. Biol. Chem. 265:4011-4019, 1990) and an apparently novel lipid, IMF-1, may regulate α M  β 2  (Hermanowski-Vosatka et al., Cell 68:341-352, 1992). Although many treatments may change integrin affinity in vitro, the mechanism(s) of physiological modulation has not been defined. 
     SUMMARY OF THE INVENTION 
     We have shown that the cytoplasmic domain of integrin molecules is involved in modulating the ligand binding activity of the integrin extracellular domain. 
     Accordingly, the invention features, in one aspect, a method for measuring the ability of a candidate compound to inhibit activation of a target integrin. In this method, a cell expressing a chimeric integrin is cultured in the presence of the candidate compound. The cell is then contacted with a ligand that binds to the reporter integrin only when the reporter integrin is activated. The level of ligand bound to the chimeric integrin in the presence of the candidate compound is a measure of the ability of the candidate compound to inhibit activation of the target integrin. In a preferred embodiment, the reporter integrin is α IIb  β 3 . In another preferred embodiment, the target integrin is selected from the group consisting of α v  ≢ 3 , α M  β 2 , α L  β 2 , α 2  β 1 , α 5  β 1 , α 6A  β 1 , α 6B  β 1 , α IIb  β 3 , and α 4  β 1 . 
     &#34;Integrin activation&#34; as used herein, is defined as the process whereby the cytoplasmic domain of the integrin stimulates the ligand binding activity of the extracellular domain. 
     A &#34;chimeric integrin&#34;, as used herein, is defined as an integrin comprising the extracellular and transmembrane domains from a reporter integrin and the cytoplasmic domain from a target integrin. Accordingly, &#34;reporter integrin&#34; is defined as an integrin from which the extracellular and transmembrane domains of a chimeric integrin are derived, while &#34;target integrin&#34; is defined as an integrin from which the cytoplasmic domain of a chimeric integrin is derived. 
     The ligand used in the screening method of the invention can be any molecule, e.g., an antibody, that binds to the reporter integrin only when the reporter is activated. In the case of the α IIb  β 3  reporter integrin, the ligand is preferably the PACl antibody or fibrinogen. 
     The cell used in the screening method of the invention is preferably one in which the target integrin is naturally expressed and activated. Cell types that can be used in the invention include, but are not limited to, leukocytes, fibroblasts, and cancer cells. Specific examples of useful cells include: Jurkat (e.g., Jurkat clone E6-1, which can be obtained from the American Type Culture Collection, Rockville, Md.; ATCC TIB 152), K562 (human erythroleukemia cells; ATCC CCL 243), CHO (Chinese Hamster Ovary cells; ATCC CCL 61), THP-1 (human monocytes; ATCC TIB 202), U937 (human histiocytic lymphoma cells; ATCC CRL 1593), WI-38 (human lung fibroblasts; ATCC CCL 75), and MG63 (human osteosarcoma cells; ATCC CRL 1427) cells. In addition, peripheral blood T cells and blood platelets, both of which can be isolated by standard methods, can be used in the invention. 
     In another aspect, the invention features a chimeric integrin molecule, as defined above. Any integrin can be used as a reporter and/or a target integrin. In a preferred embodiment, the reporter integrin is α IIb  β 3 . Preferred target integrins include, but are not limited to α v  β 3 , α M  β 2 , α L  β 2 , α 2  β 1 , α 5  β 1 , α 6A  β 1 , α 6B  β 1 , α IIb  β 3 , and α 4  β 1 . 
     The invention also features a method of inhibiting the ligand binding activity of an integrin molecule in a cell involving introducing into the cell a compound which inhibits integrin activation. Preferably, the compound used to inhibit integrin activation is a small organic molecule, and the cell in which the compound inhibits integrin activation is a leukocyte, a platelet, or a cancer cell. 
     The inhibitors of the invention can be used to treat mammals, such as humans, who have or are at risk of developing an unwanted immune response, e.g., inflammation, or an immune response resulting from autoimmune disease or the presence of a transplanted organ or tissue. In addition, the inhibitors can be used to treat patients who have, or are at risk of developing cancer, as well as to treat patients who have, or are at risk of developing a thrombus. 
     The invention provides a rapid and facile method for identifying inhibitors of integrin activation in which a large number of compounds can be screened. The use of chimeric integrins allows inhibitors for target integrins to be identified even in cases in where an activation-specific ligand for the target integrin has not been identified. α IIb  β 3  is a particularly useful reporter integrin, as the activation-specific ligands for α IIb  β 3 , PACl and Fg, do not bind to other commonly expressed tissue integrins. 
     Other features and advantages of the invention will become apparent from the following detailed description, and from the claims. 
    
    
     DETAILED DESCRIPTION 
     The drawings are first described. 
     Drawings 
     FIG. 1A is a graph showing the levels of fibronectin bound to CHO cells, K562 cells, and K562 cells in the presence of the &#34;activating&#34; antibody 8A2. The graph also shows the levels of fibronectin bound to the above-listed cells in the presence of an anti-α 5  antibody. 
     FIG. 1B is a graph of results from flow cytometry analysis of K562 and CHO cells stained with an irrelevant mouse IgG (dotted line), an anti-β 1  antibody (solid line), and an anti-α 5  antibody (dashed line). 
     FIG 1C is a graph showing the levels of fibronectin bound to resting CHO cells; CHO cells in the presence of the anti-α 5  antibody (PB1); CHO cells incubated with deoxyglucose and sodium azide (DOG/Az); and CHO cells washed free of deoxyglucose and sodium azide, and returned to glucose medium (Wash+Glc). 
     FIG. 2 is a listing of the amino acid sequences (SEQ ID NOs: 1-12) of wild type and variant integrin cytoplasmic domains. 
     FIG. 3A is a graph of results from flow cytometry analysis of CHO and K562 cells stably transfected with chimeric integrins containing the cytoplasmic domains of α5 and β 1  and the extracellular domains of α IIb  and β 3 . 
     FIG. 3B is an autoradiogram of immunoprecipitates of lysates prepared from surface iodinated wild type K562 cells (None) or stable K562 transfectants expressing the α subunit indicated at the tops of the lanes (α IIb  α 5  =α 5  cytoplasmic domain chimera), fractionated by SDS-PAGE. The immunoprecipitations were carried out with antibodies specific for the specific integrin domains indicated below each of the lanes. 
     FIG. 3C is an illustration of the locations of the 2bsf, 2bcyt and α 5  cyt primers used for PCR analysis. The transmembrane (TM: crosshatched), 3&#39; untranslated (3&#39;UT:stippled), and cytoplasmic and extracellular domain (clear) sequences are indicated. Also shown is a photograph of an agarose gel upon which amplified products were fractionated, with arrows indicating the positions of the 393 and 294 bp bands. The transfectant type is listed above, while the 3&#39; primer used is indicated below. 
     FIG. 4A is a graph of results from flow cytometry analysis of PACl binding to stable CHO transfectants expressing the α 5  and α 1  cytoplasmic domain chimeras in the absence (solid line) and presence (dotted line) of 2 mM GRGDSP (SEQ ID NO: 13) (Inhibitors: deoxyglucose+NAN 3 ). 
     FIG. 4B is a graph showing the levels of Fg bound to stable CHO transfectants expressing the cytoplasmic domains indicated below the graph. 
     FIG. 5A is a graph of results from flow cytometry analysis of PACl binding to CHO cells transiently transfected with subunits comprised of the extracellular and transmembrane domains of α IIb  and β 3  joined to the indicated cytoplasmic domains. Binding was analyzed in the absence (solid line) or presence (dotted line) of GRGDSP (SEQ ID NO: 13) peptide. 
     FIG. 5B is a graph of results from flow cytometry analysis of PACl binding to CHO cells CHO cell lines stably expressing recombinant α IIb  β 3  chimeras containing the indicated cytoplasmic domains. Binding was analyzed in the absence (solid line) or presence (dotted line) of GRGDSP (SEQ ID NO: 13) peptide. 
     FIG. 6A is a graph showing the activation index for CHO cells transiently transfected with chimeric α subunits consisting of extracellular and transmembrane α IIb  with the indicated cytoplasmic domains, and β 3 . 
     FIG. 6B is a graph showing the activation index for CHO cells transiently transfected with chimeric α subunits containing the indicated cytoplasmic sequences and a β 3  subunit in which the cytoplasmic domain was truncated (β 3  Δ724), contained the S 752  →P mutation (S752P), or had been exchanged for the homologous region of β 1 . 
     FIG. 7A is a series of graphs of the results of flow cytometry analysis of PACl binding to stable CHO cell lines co-transfected with α IIb  containing the indicated α cytoplasmic domain with wild type β 3 . 
     FIG. 7B is a series of graphs of the results of flow cytometry analysis of PACl binding to CHO cells transiently transfected with chimeras of the extracellular and transmembrane domains of α IIb  β 3  joined to the indicated cytoplasmic domains. 
     FIG. 8 is an illustration of a model for affinity modulation of integrins. 
    
    
     INHIBITORS 
     We have shown that the integrin cytoplasmic domain plays a role in activating the ligand binding activity of the integrin extracellular domain. Integrin-ligand binding interactions play central roles in a number of physiological processes, including activation of the immune response, inflammation, hemostasis, thrombosis, cell migration, and tumor cell invasion. Thus, inhibiting integrin activation can be useful in modulating these processes. 
     Inhibition of the ligand binding activity of an integrin can be achieved by administering a compound that inhibits integrin activation. Such a compound can be identified by methods ranging from rational drug design to screening of random compounds. The latter method is preferable, as a simple and rapid assay for carrying out this method is available. Small organic molecules are desirable candidate compounds for this analysis as frequently these molecules are capable of passing through the plasma membrane so that they can potentially act on integrin cytoplasmic domains. 
     The screening of small, membrane-permeable organic molecules for the ability to inhibit integrin activation is carried out as follows. First, compounds are tested in cultured cells expressing chimeric integrin molecules. Second, compounds which test positive in the cultured cells are tested in an animal model system. 
     Chimeric integrin molecules used in the cell culture-based screening method contain the extracellular and transmembrane domains of a reporter integrin fused to the intracellular domain of a target integrin. The preferred reporter integrin of the invention is α IIb  β 3 , as its known ligands, PACl (Shattil et al., J. Biol. Chem. 260:11107-11114, 1985) and Fg, bind specifically to activated α IIb  β 3 , and not to inactive α IIb  β 3 , or other integrins. Other integrins may also be used as reporter integrins in the invention, provided that an activation-specific ligand is available. Preferred target integrins include, but are not limited to α v  β 3 , α M  β 2 , α L  β 2 , α 2  β 1 , α 5  β 1 , α 6A  β 1 , α 6B  β 1 , α IIb  β 3  and α 4  β 1 . Chimeric integrins can be generated using standard methods of molecular biology (Sambrook et al., Molecular Cloning: A Laboratory Manual,  2nd, Cold Spring Harbor Laboratory Press, 1989). 
     The cell culture assay for identifying inhibitors of integrin activation involves culturing cells expressing a chimeric integrin in the presence or absence of a candidate compound, and determining the level of reporter integrin activation by contacting the cell with a ligand that binds to the reporter integrin only when the reporter integrin is activated. A compound tests positive in the cell culture assay if the amount of ligand bound to the reporter integrin in the presence of the compound is less than the amount bound in its absence. 
     Any reagent that binds to a reporter integrin specifically when it is activated, e.g., an activation-specific antibody, can be used as the ligand in the screening method of the invention. In the case of the α IIb  β 3  reporter integrin, PACl and Fg are preferable ligands. The ligand can be tagged with a label, e.g., an enzymatic, chromogenic, radioactive, or luminescent label, which can be detected using standard methods in the art, including flow cytometry, direct radio-ligand binding assays, and ELISA. Binding of the ligand to the reporter integrin can also be detected by the use of antibodies which specifically bind to the ligand which can be detected by standard methods. 
     Compounds found to affect integrin activation in the cell culture assay can be further tested in animal model systems. A candidate compound can be administered to an appropriate animal, e.g., an immunocompetent mouse which has a non-MHC matched skin graft, and the effect of the compound can be determined by monitoring the immune response of the mouse. 
     Role of Cytoplasmic Tail in Integrin Activation 
     Cell type-specific and energy-dependent affinity modulation of integrin α 5  β 1 . 
     There is evidence for cell type-specific control of the adhesive function of integrins. To begin to investigate the cell type-specific control of ligand binding affinity, we analyzed the binding of soluble fibronectin (Fn) to cells expressing integrin α 5  β 1 . The cells analyzed fell into two groups: those that bound Fn with only low affinity (Kd&gt;1  μM), e.g., K562, THP1, U937, and peripheral blood T cells, and those that bound with moderate affinity (Kd˜100 nM), e.g., CHO, WI-38, and MG63 cells. The low affinity α 5  β 1  integrin was intrinsically functional since it bound Fn after &#34;activation&#34; with the 8A2 monoclonal antibody (Faull et al., J. Cell Biol. 121:155-162, 1993). Specificity of Fn binding to high affinity α 5  β 1  was verified by inhibition with an anti-α 5  antibody (FIG. 1A;  125  I-Fn (50 nM) was incubated at 22° C. with CHO or K562 cells. After 30 minutes, bound Fn was assessed by centrifugation through a sucrose cushion, as described below. α 5  β 1  -specific binding was established by blocking Fn binding to the CHO cells with PB1, an anti-hamster α 5  antibody. Binding to K562 cells was induced by addition of 20 nM &#34;activating&#34; antibody (8A 2) and was inhibited by the anti-α 5  antibody (BIIG2). The levels of surface expression of α 5  β 1  in the two cell types was also determined (FIG. 1B; CHO and K562 cells were stained with irrelevant mouse IgG (dotted line), an anti-β 1  antibody (K562:SA2, CHO:7E2) (solid line), or an anti-α 5  antibody (K562:BIIG2, CHO:PB1) (dashed line) and then analyzed by flow cytometry as described below). 
     To determine whether spontaneous high affinity Fn binding to α 5  β 1  is an active process, we treated CHO cells with a combination of inhibitors of oxidative phosphorylation (NAN 3 ) and anaerobic glycolysis (2-deoxyglucose). This resulted in loss of specific high affinity Fn binding. This effect was partially reversible since washout of the metabolic inhibitors resulted in restoration of 75% of the high affinity binding (FIG. 1C; the binding of  125  I-Fn to CHO cells (Resting), to cells incubated in medium containing 2 mM deoxyglucose and 0.1% sodium azide (DOG/Az), or to cells washed free of these inhibitors and returned to glucose-containing medium (Wash+Glc) was determined. Specificity of binding to α 5  β 1  was verified by inhibition with the PB1 antibody). Thus, high affinity Fn binding to integrin α 5  β 1  is cell type-specific and an active cellular process. 
     The cytoplasmic domains of α 5  β 1  confer an energy-dependent high affinity state on α IIb  β 3  in some cells but not others. 
     To determine whether the cytoplasmic domains of α 5  β 1  are involved in cell type-specific affinity modulation, we generated chimeras in which the cytoplasmic domains of α IIb  and β 3  were replaced with the corresponding sequences from α 5  and β 1  (FIG. 2; Amino acid sequences of wild type and variant integrin cytoplasmic domains. Single letter amino acid code is used. The arrows underneath the α IIb  (residue 990) and β 3  (residue 727) sequences denote the position at which chimeric cytoplasmic domains were joined to the extracellular and transmembrane domains of α IIb  and β 3 . The position of stop codons producing cytoplasmic truncations are noted by triangles, while the S 752  →P point mutation in β 3  is indicated. The residues deleted in the α L  Δ cytoplasmic domain are overlain by the heavy line). The α and β chimeras were co-transfected into CHO or K562 cells, and the affinity state of the extracellular α IIb β 3  reporter group was assayed by binding of PACl, an antibody specific for the high affinity state of α IIb  β 3  (Shattil et al., J. Biol. Chem. 260:11107-11114, 1985). The double chimera bound PACl when it was expressed in CHO cells. Since wild-type α IIb  β 3  does not bind PACl when expressed in CHO cells (O&#39;Toole et al., Cell Regulation 1:883-893, 1990), it is concluded that the α 5  β 1  cytoplasmic domains conferred the high affinity state on α IIb  β 3 . In sharp contrast, PACl did not bind to the double chimera in K562 cells. However, PACl bound after addition of an activating antibody, anti-LIBS6, confirming that the ligand binding site was intact (FIG. 3A; CHO or K562 cells were stably transfected with chimeras containing the cytoplasmic domains of α 5  and β 1  and the affinity state of the α IIb  β 3  extracellular domain was assayed by its ability to bind PACl in the absence (solid line) or presence (dotted line) of 1 mM GRGDSP (SEQ ID NO: 13). Depicted are flow cytometry histograms. The K562 transfectants specifically bound PACl only after incubation with 6 [M activating antibody, anti-LIBS 6). Thus, the capacity of cell type-specific elements to modulate affinity depends on the integrin cytoplasmic domains. 
     Since K562 cells express endogenous α IIb  under certain conditions (Burger et al., Exp. Cell Res. 202:28-35, 1992), it was necessary to verify that all of the α IIb  expressed in the α chimera transfectants contained the α 5  cytoplasmic domain. Immunoprecipitation of surface iodinated α chimera transfectants with an anti-α 5  cytoplasmic domain antibody isolated polypeptides corresponding to transfected α IIb  and β 3  chimeras and endogenous α IIb  β 1 . In contrast, an anti-α II   b  cytoplasmic domain antibody immunoprecipitated no labeled polypeptides. An anti-α 5  cytoplasmic antibody precipitated only endogenous α 5  β 1  from wild-type α IIb  β 3  transfectants (FIG. 3B; Immunoprecipitation analysis of K562 transfectants. Wild type K562 cells (None) or stable transfectants expressing the α subunit noted in the figure (α IIb  β 5  =α 5  cytoplasmic domain chimera) were surface iodinated, lysed, and immunoprecipitated with polyclonal antibodies specific for the α 5  and α IIb   cytoplasmic domains, or with a monoclonal antibody reactive with the extracellular domain of α IIb  β 3  (2G12). Immunoprecipitates were resolved by SDS-PAGE and constituent polypeptides were visualized by autoradiography). 
     In addition, we confirmed fidelity of expression at the mRNA level. Reverse transcriptase-polymerase chain reaction was performed using a 5&#39; primer specific for the extracellular domain of α IIb  and 3&#39; primers specific for cytoplasmic domains of α IIb  or α 5 . A specific 393 base pair band was observed from α chimera transfectants when primed with the 3&#39; α IIb  oligonucleotide. No bands were observed when inappropriate 3&#39; primers were used (FIG. 3C; Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. RT-PCR was performed as described below with the 5&#39; 2bsf primer and 3&#39; primers specific for α IIb  or α 5  3 &#39; untranslated sequences, and the amplified products were analyzed by agarose gel electrophoresis). 
     As was shown in FIG. 1, high affinity Fn binding to α 5  β 1  depends on active cellular metabolism. We therefore analyzed the effects of NaN 3  and 2-deoxyglucose on the affinity state of the double chimera in CHO cells. These inhibitors blocked both PACl (FIG. 4A; Stable CHO transfectants expressing the α 5  and β 1  cytoplasmic domain chimeras were assayed for PACl binding in the absence (solid line) and presence (dotted line) of 2 mM GRGDSP (SEQ ID NO: 13) by flow cytometry. Transfectants incubated with 2 mg/ml deoxyglucose and 0.1% NaN 3  (Inhibitors), as described below, manifested loss of specific binding. Addition of 6 μM anti-LIBS2 (Inhibitors+Anti-LIBS2) or washout of these inhibitors (Inhibitors+washout) and return to glucose-containing medium reconstituted specific PAC1 binding) and Fg (FIG. 4B; Stable CHO transfectants expressing the cytoplasmic domains noted below the graph were analyzed for Fg binding as described below. Constitutive binding to transfectants expressing the α 5  and β 1  chimeras was inhibited by  2 mg/ml deoxyglucose plus 0.1% NAN 3  ; binding to transfectants expressing the α IIb  Δ991 or α L  Δ variant was not inhibited (see below)) binding. Anti-LIBS2, an activating antibody (Frelinger et al., J. Biol. Chem. 266:17106-17111, 1991), restored high affinity binding. Furthermore, the metabolic blockade was reversible since high affinity ligand binding reappeared after the inhibitors were washed out (FIG. 4A). These results show that α 5  β 1  cytoplasmic sequences confer a cell type-specific, energy-dependent, high affinity state on the extracellular domain of α IIb  β 3 . 
     Both α and β cytoplasmic domains are involved in affinity modulation. 
     To determine which cytoplasmic domain specified the high affinity state in CHO cells, we transfected each subunit chimera with a complementary wild-type subunit. Transfectants expressing both α and β chimeras or expressing the chimeric α but wild-type β 3  subunits bound PACl. In contrast, cells expressing the β chimera with wild-type α IIb  were in a low affinity state and bound PACl only after addition of anti-LIBS2 (FIG. 5A; CHO cells were transiently transfected with subunits comprised of the extracellular and transmembrane domains of α IIb  and β 3  joined to the indicated cytoplasmic domains. The affinity state of the extracellular portion of α IIb  β 3  was assessed by PACl binding. Binding was analyzed in the absence (solid line) or presence (dotted line) of GRGDSP (SEQ ID NO: 13) peptide. Graphs of cells incubated in the presence of 6 μM anti-LIBS2 are depicted in the lower panels. Specific PACl binding is present in both transfectants containing the β 5  cytoplasmic domain irrespective of the presence of either the β 3  or β 1  cytoplasmic domain on the β 3  subunit. In contrast, PACl specifically bound to those transfectants containing the α IIb  cytoplasmic domain only in the presence of the activating antibody, anti-LIBS2). These results show that α  cytoplasmic sequences are involved in specifying affinity state. 
     To find out if the β subunit was also involved in specifying the high affinity state in CHO cells, we constructed two β 3  cytoplasmic variants, β 3  Δ724 and β 3  (S 752  →P). The former is a truncation mutant that ends at D 723 , while the latter contains a single nucleotide alteration resulting in a Ser 752  →Pro substitution (FIG. 2). These β 3  cytoplasmic domain mutants were co-transfected with the α chimera. In contrast to wild-type β 3 , coexpression of either β 3  variant with chimeric α resulted in a receptor that failed to bind PACl constitutively (FIG. 5B; Stable CHO cell lines expressing recombinant α IIb  β 3  chimeras containing the noted cytoplasmic domains were reacted with PACl and bound antibody was detected by flow cytometry as described below. Binding was analyzed in the absence (solid line) or presence (dotted line) of GRGDSP (SEQ ID NO: 13) peptide. The intrinsic functionality of each construct was assessed by PACl binding in the presence of 6 μM anti-LIBS2  (lower panels). A β 3  cytoplasmic truncation (Δ724) and single amino acid substitution(S 752  →P) both abolished the constitutive high affinity state conferred by the cytoplasmic domain of α 5 ). Thus, the cytoplasmic domain of the β subunit as well as the α subunit is involved in affinity modulation. 
     Regulation of integrin affinity by the α subunit cytoplasmic domain is α subunit-specific 
     These data established that the cytoplasmic domains of α IIb  and α 5  specify different affinity states in CHO cells; α IIb  the low and α 5  the high affinity state. To determine whether there are consensus &#34;activation&#34; sequences, we constructed chimeras with the cytoplasmic domains of six additional α subunits and analyzed their affinity state after co-transfection with β 3  into CHO cells. The α cytoplasmic domains of three other β 1  family members (α 2 , α 6  A, α 6  B) conferred PACl binding (FIG. 6A), while those chimeras containing α subunit cytoplasmic domains from β 2  (α M , α L ) or β 3  (α v ) families did not (FIG. 6A; Chimeric α subunits consisting of extracellular and transmembrane α IIb  with the indicated cytoplasmic domain were transiently co-transfected with β 3  into CHO cells. PACl binding was quantified by flow cytometry and the activation index was calculated as: 
     
         100*(F.sub.O -F.sub.R)/F.sub.R 
    
     where: 
     F O  =Mean Fluorescence Intensity in the absence of inhibitor 
     F R  =Mean Fluorescence Intensity in the presence of GRGDSP 
     (SEQ ID NO: 13). Depicted are the Mean±S.D. of at least 3 independent experiments for each α chimera). The same result was obtained with the β chimeras containing cytoplasmic domains of the relevant β subunit partner (β 1  for α 2 , α v , α 6  A, and α 6  B or β 2  for α L  and α M ). Similar to the α 5  chimera, constitutive PACl binding was also dependent upon the β cytoplasmic domain. It was lost when the β 2 , α 6  A, or α 6  B chimeras were co-transfected with β 3  Δ724 or βS752P (FIG. 6B; α subunit chimeras containing the indicated cytoplasmic sequences were co-transfected with a β 3  subunit in which the cytoplasmic domain was truncated (β 3  Δ724), contained the S 752  →P mutation (S752P), or had been exchanged for the homologous region of β 1 . PACl binding was analyzed as described for FIG. 6A. Mean±S.D. of at least 3 independent experiments for each α β pair are depicted). Thus, the α subunit cytoplasmic domain designates integrinspecific affinity differences. The β subunit cytoplasmic domain may be permissive for the high affinity state. 
     Deletion of conserved α cytoplasmic sequences results in high affinity ligand binding that is independent of metabolic energy and the β subunit cytoplasmic domain 
     We previously reported that constitutive ligand binding to α IIb  β 3  results from a truncation of the cytoplasmic domain of α IIb  (O&#39;Toole et al., Science 254:845-847, 1991). To identify the important deleted α IIb  cytoplasmic residues, we generated additional variants. Integrin α subunit cytoplasmic domains contain a highly conserved GFFKR (SEQ ID NO: 14) sequence at their NH 2  -termini (FIG. 2). As previously reported (O&#39;Toole et al., Science 254:845-847, 1991; Ylanne et al., J. Cell Biol. 122:223-233, 1993), the α IIb  Δ911 truncation eliminates this motif and results in constitutive PACl binding whereas a truncation after the GFFKR (SEQ ID NO: 14) (α IIb  Δ996) does not (FIG. 7A). This pinpoints the conserved motif as a regulator of integrin affinity. To test this idea, we removed the LGFFK (SEQ ID NO: 15) residues from the cytoplasmic domain of an α L  cytoplasmic domain chimera (FIG. 2). This chimera was selected because it possesses the longest α cytoplasmic domain. Coexpression of this chimeric internal deletion mutant (α L  Δ) in CHO cells with β 3  resulted in high affinity PACl binding (FIG. 7B). Finally, to further exclude contributions from downstream α sequences, we generated a variant that contains a 24-residue random cytoplasmic sequence (FIG. 2). This construct (α Ra ) also conferred high affinity binding when expressed in CHO cells with wild-type β 3  (FIG. 7A; Stable CHO cell lines were established by co-transfection of α IIb  containing the α cytoplasmic domain indicated in the figure with wild type β 3 . PACl binding in the absence (solid line) and presence (dotted line) of GRGDSP (SEQ ID NO: 13) was assessed by flow cytometry. The α IIb  Δ991 transfectant, which lacks GFFKR (SEQ ID NO: 14), specifically binds PACl. In contrast the α IIb  Δ996 transfectant, which retains GFFKR (SEQ ID NO: 14), binds only after &#34;activation&#34; with anti-LIBS2. Replacement of the α IIb  cytoplasmic domain with random sequence also induces PACl binding (α Ra )). 
     To gain insight into the mechanisms of high affinity binding conferred by the GFFKR (SEQ ID NO: 14) deletion mutants, we examined the requirements for cellular metabolism and β cytoplasmic sequences. In contrast to the constitutively active chimeras, high affinity PACl binding in the GFFKR (SEQ ID NO: 14) deletion variants was maintained when they were coexpressed with the truncated β 3  subunit (FIG. 7B). In addition, in contrast to transfectants expressing constitutively active α chimeras, transfectants expressing the GFFKR (SEQ ID NO: 14) deletion retained high affinity for Fg and PACl (FIG. 7B) when treated with the metabolic inhibitors NaN 3  and 2-deoxyglucose. Finally, the α L  Δ mutant conferred cell-type independent activation, since it was active in K562 (FIG. 7B; CHO cells were transiently transfected with chimeras of the extracellular and transmembrane domains of α IIb  β 3  joined to the cytoplasmic domains indicated in the figure. Specific PACl binding to the population of cells expressing α IIb  β 3  was detected as in FIG. 7A. A GFFKR (SEQ ID NO: 14) &#34;loop out&#34; mutant manifested PACl binding (α L Δ β 3 ) that was maintained in the presence of 0.1% NaN 3  and 2 mM 2-deoxyglucose (inhibitors). This treatment abolished ligand binding to an α IIb  β 3  chimera bearing the cytoplasmic domain of α 5  β 1 . High affinity state was also maintained despite an extensive deletion of the β 3  cytoplasmic domain (α L Δ β.sub. Δ724) that disrupted PACl binding to the α 5  β 1  chimera. Similar results were obtained with α IIb  Δ991 and α Ra  transfectants. A stable K562 cell line bearing the a GFFKR (SEQ ID NO: 14) deletion mutant specifically bound PACl (α L Δ β 3 ), but the α 5  β 1  chimera was not active in these cells) and COS, as well as in CHO cells. Thus, deletions in the highly conserved GFFKR (SEQ ID NO: 14) motif resulted in a cell type-independent high affinity state that was resistant to metabolic inhibitors and truncation of the β subunit. 
     Experimental Procedures: 
     Antibodies and reagents. 
     The anti-α IIb  β 3  antibody D57 was produced using previously described methods (Frelinger et al., J. Biol. Chem. 265:6346-6352, 1990). It binds to Chinese hamster ovary (CHO) cells transfected with α IIb  β 3 , but not α v  β 3 , and does not block Fg binding to α IIb  β 3 . This antibody was biotinylated with biotin-N-hydroxy-succinimide (Sigma Chemical, St. Louis, Mo.) according to manufacturer&#39;s directions. The α IIb  β 3  complex specific antibody, 2G12 (Plow et al., Blood 66:724-727, 1985), was used as dilutions of ascites fluid. The anti-hamster α 5  (PB1) and anti-β 1  (7E2) antibodies, the β 1  activating antibody, 8A2 (Kovach et al., J. Cell Biol. 116:499-509, 1992); a human anti-α antibody, BIIG2 (Werb et al., J. Cell Biol. 109:877-889, 1989); a polyclonal anti-peptide antibody against the cytoplasmic domain of human α 5  (Hynes et al., J. Cell Biol. 109:409-420, 1989); anti-LIBS6, anti-LIBS2, and anti-α IIb  cytoplasmic domain antibodies (Frelinger et al., J. Biol. Chem. 265:6346-6352, 1990; O&#39;Toole et al., Science 254:845-847, 1991); and PACl (Shattil et al., J. Biol. Chem. 260:11107-11114, 1985) have been described previously. Glucose and 2-deoxyglucose were purchased from Sigma and sodium azide was purchased from Fisher Scientific Co. (Pittsburgh, Pa.). The peptide GRGDSP (SEQ ID NO: 13) was obtained from Peninsula Laboratories (Belmont, Calif.). Its purity and composition were verified by high performance liquid chromatography and fast atom bombardment mass spectroscopy. 
     Cell culture and transfection. 
     The human cell lines K562, U937, W1-38, and MG63 were obtained from the American Type Culture Collection (ATCC; Rockville, Md.) and maintained in RPMI 1640 media (Biowhittaker, Walkersville, Md.) containing 10% fetal bovine serum (Biowhittaker, Walkersville, Md.) 1% glutamine (Sigma) and 1% penicillin and streptomycin (Sigma). THP-1 cells (ATCC; Rockville, Md.) were maintained in the same medium with the addition of 10 mM Hepes and 20 mM 2-mercaptoethanol. Chinese hamster ovary (CHO) cells (ATCC; Rockville, Md.) were maintained in DMEM media (Biowhittaker; Walkersville, MD) with 10% fetal calf serum, the above noted antibiotics, and 1% non-essential amino acids (Sigma). Human T lymphocytes were purified from peripheral blood of normal donors by centrifugation on a Ficoll-Paque gradient (Pharmacia Fine Chemicals, Piscataway, N.J.), panning for monocytes on serum-coated dishes, and passage over a nylon wool column. 
     CHO cells were transiently transfected by electroporation. Cells in log phase growth were harvested with trypsin (Irvine Scientific), washed with PBS, and combined with appropriate cDNAs (10 μg of each subunit). 3×10 7  cells in 0.5 ml of growth media were electroporated at 350 volts, 960 μF, in a BTX (BTX, San Diego, Calif.) electroporator. Media were changed after 24 hours and cells analyzed for surface expression, or PACl binding after 48 hours. Stable CHO transfectants were established as above with co-transfection of 0.6 μg of CDNeo. After 48 hours, these cells were selected for 2 weeks in 700 μG418 (Gibco) and clonal lines were established by single cell sorting in a FACStar (Becton Dickinson). Stable K562  transfectants were established by electroporation of 1×10 7  cells in 0.8 ml of PBS at 300 volts and 500 μF. After 48 hours the cells were maintained in media containing 1 mg/ml G418, and clonal lines established by limiting dilution cloning. 
     Flow Cytometry 
     Surface expression of integrins was analyzed by flow cytometry with specific antibodies as described (Loftus et al., Science 249:915-918, 1990; O&#39;Toole et al., Blood 74:14-18, 1989). Briefly, 5×10 5  cells were incubated on ice for 30 minutes with primary antibody, washed, and incubated on ice for 30 minutes with an FITC-conjugated goat anti-mouse (Tago, Burlingame, Calif.) secondary antibody. Cells were pelleted, resuspended, and analyzed on a FACScan (Becton Dickinson). PACl binding was analyzed by two color flow cytometry. Cell staining was carried out in Tyrode&#39;s buffer (Ginsberg et al., Blood 55:661-668, 1980) containing 2 mM MgCl 2  and CaCl 2  and 1 mg/ml BSA (Sigma) and dextrose. Single cell suspensions were obtained by harvesting with 3.5 mM EDTA, incubating for 5 minutes in 1 mg/ml TPCK trypsin (Worthington), and diluting with an equal volume of Tyrode&#39;s buffer containing 10% fetal calf serum and 0.1% soybean trypsin inhibitor (Sigma). After washing, 5×10 5  cells were incubated in a final volume of  50 μl containing 0.1% PACl ascites fluid in the presence or absence of 1 mM GRGDSP (SEQ ID NO: 13) peptide. After a 30 minute incubation at room temperature, cells were washed with cold Tyrode&#39;s buffer and then incubated on ice with biotinylated antibody D57. After 30 minutes cells were washed and then incubated on ice with Tyrode&#39;s buffer containing 10% FITC-conjugated goat anti-mouse IgM (Tago) and 4% phycoerythrin-streptavidin (Molecular Probes Inc., Junction City, Oreg.). Thirty minutes later, cells were diluted to 0.5 ml with Tyrode&#39;s buffer and analyzed on a FACScan (Becton Dickinson) flow cytometer as described (O&#39;Toole et al., Cell Regulation 1:883-893, 1990). PACl binding (FITC staining) was analyzed only on a gated subset of cells positive for α IIb  β 3  expression (phycoerythrin staining). To define affinity state, histograms depicting PACl staining in the absence or presence of 1 mM GRGDSP (SEQ ID NO: 13) were superimposed. Since RGD peptides are inhibitors of PACl binding to α IIb  β 3  (Bennett et al., J. Biol. Chem. 263:12948-12953, 1988), a rightward shift in the histogram in the absence of RGD peptide is indicative of the presence of high affinity α IIb  β 3  integrin. To compare the effects of multiple α subunits, pooling of data involving experiments from different days was required. To do this, a numerical activation index was defined as: 
     
         100*(F.sub.O -F.sub.R)/F.sub.R 
    
     where: 
     F O  =Mean Fluorescence Intensity in the absence of inhibitor, and 
     F R  =Mean Fluorescence Intensity in the presence of GRGDSP (SEQ ID NO: 13). 
     DNA Constructs 
     The generation of CDM8 constructs encoding α IIb , α IIb  Δ991, α IIb  Δ996, β 3 , and β 3  Δ728 has been previously described (O&#39;Toole et al., Blood 74:14-18, 1989; O&#39;Toole et al., Science 254:845-847, 1991; Ylanne et al., J. Cell Biol. 22:223-233, 1993). The β 3  truncation, Δ724, and amino acid substitution, S 752  →p, were first generated in BS3a (O&#39;Toole et al., Blood 74:14-18, 1989) by oligonucleotide-directed mutagenesis (Kunkel, Proc. Natl. Acad. Sci. USA 82:488-492, 1985), digested with HincII to isolate coding sequences, ligated to BstXI linkers (InVitrogen) and subcloned into the BstXI sites of CDM8. The β 3  chimera, containing the β 1  cytoplasmic domain, was constructed by first generating an EcoRI site at bases 2387-2392 of β 1  cDNA sequence. After HindIII digestion, a 400 bp fragment containing the complete β 1  cytoplasmic domain and partial 3&#39; non-coding sequences was isolated and subcloned into the HindIII site of CDM8. This construct was then digested with EcoRI and ligated with a 2.2 kb EcoRI fragment from CD3a (O&#39;Toole et al., Blood 74:14-18, 1989) containing its transmembrane and extracellular domains. β 2  cytoplasmic sequences were first isolated by the polymerase chain reaction (PCR) from a β 2  cDNA and then subcloned into the MluI and XhoI sites of CDM8. The β 2  cytoplasmic domain chimera was then generated by digestion with MluI and HindIII and ligation with a corresponding MluI-HindIII fragment from CD3a containing its extracellular and transmembrane sequences. Chimeric α subunits were generated following a previously described strategy (O&#39;Toole et al., Science 254:845-847, 1991). Cytoplasmic sequences from α v , α M , α 2 , α 6  A, and α 6  B were isolated from the appropriate cDNA clones by PCR (Loftus et al., Science 249:915-918, 1990). Amplified products were digested with HindIII and XbaI and subcloned into HindIII and XbaI cut CDM8. After digesting with HindIII, these constructs were ligated with a HindIII fragment from CD2b (O&#39;Toole et al., Blood 74:14-18, 1989) containing its extracellular and transmembrane domains. PCR oligonucleotides for α L  Δ were designed to omit the VGFFK (SEQ ID NO: 16) sequence. Its construction followed the procedure for other α chimeras. The α Ra  variant was made by first generating a SalI site in CD2b coding sequences corresponding to bases 3061-3066. This vector was then digested with SalI and XbaI and ligated to a SalI-XbaI Bluescript vector sequence (bases 674-731). All constructs were verified by DNA sequencing and purified by CsC1 centrifugation before transfection. Oligonucleotides were synthesized on a Model 391 DNA Synthesizer (Applied Biosystems). 
     Ligand Binding 
     The binding of  125  I-Fg or  125  I-Fn to cultured cells was carried out as described (O&#39;Toole et al., Cell Regulation 1:883-893, 1990; Faull et al., J. Cell Biol. 121:155-162, 1993). Cells were harvested with EDTA and trypsin as described above for flow cytometry analysis, and resuspended in a modified Tyrode&#39;s buffer (150 mM NaCl, 2.5 mM KCl, 2 mM NaHCO 3 , 2 mM MgCl 2 , 2 mM CaCl 2 , 1 mg/ml BSA, and 1 mg/ml dextrose). A typical assay included 120 μl of cells (2×10 6  cells per tube), 40 μl of radiolabelled protein, and 40 μl of inhibitor (GRGDSP (SEQ ID NO: 13) peptide, blocking antibodies) or agonist (activating antibody). After 30 minutes at room temperature 50 μl aliquots were layered in triplicate on 0.3 ml of 20% sucrose and centrifuged for 3 minutes at 12,000 rpm.  125  I-labelled protein associated with the cell pellet was determined by scintillation spectrometry. Non-saturable binding was determined in the presence of 2 mM GRGDSP (SEQ ID NO: 13) peptide. Data were fit to equilibrium binding models by the nonlinear least squares curve-fitting LIGAND program (Munson and Rodbard, Anal. Biochem. 107:220-239, 1980). In binding experiments utilizing metabolic inhibitors, the cells were first incubated with 2 mg/ml 2-deoxyglucose and 0.1% sodium azide for 30 minutes at room temperature before addition of radiolabelled ligand. In washout experiments, cells treated in this way were washed, incubated with Tyrode&#39;s buffer containing 1 mg/ml dextrose for 30 minutes at room temperature, and then analyzed for ligand binding. 
     Immunoprecipitation 
     Transfectants were surface labelled by the Iodogen method according to the manufacturer&#39;s instructions (Pierce Chemical) and solubilized in lysis buffer (10 mM Hepes (pH 7.5), 0.15M NaCl, 50 mM octylglucoside, 1 mM CaCl 2 , 1 mM MgCl 2 , 1 mM phenylmethylsulfonyl fluoride, 0.1 mM leupeptin, and 10 mM N-ethylmaleimide). Cell extracts were immunoprecipitated with polyclonal antiserum directed against the α IIb  or α 5  cytoplasmic domains, and a monoclonal antibody against the α IIb  β 3  complex (2G12). The antibodies were attached to preswollen protein A-Sepharose beads (Pharmacia LKB Biotechnology Inc.) by incubation at 4° C. overnight. The antibody-conjugated Sepharose beads were washed, pelleted by centrifugation, and then incubated with the detergent lysates from the surface labelled cells overnight with shaking. The Sepharose beads were washed extensively in lysis buffer, resuspended in sample buffer (Laemmli, Nature 227:680-685, 1970), and boiled for 5 minutes. After centrifugation, the precipitated proteins were resolved by SDS-PAGE (non-reducing, 7.5% acrylamide gels). Gels were dried and radiolabelled polypeptides were visualized by autoradiography. 
     Polymerase Chain Reaction 
     Total RNA was isolated from 10 6  transfected cells using the RNAzol reagent (Cinna Biotecx). First strand cDNA synthesis from 5 μg of RNA was performed with the cDNA cycle kit (Invitrogen, San Diego, Calif.) using oligo dT as a primer. Coding sequences downstream of the α IIb  transmembrane region were specifically amplified with a 5&#39; primer specific for transmembrane α IIb  (2bsf: CGGGCCTTGGAGGAGAGGGCCATTC (SEQ ID NO: 17)) and 3&#39; primers specific for the cytoplasmic sequences of α IIb  (α IIb  cYt: CTCTGTTGGGAGGGAAACGA (SEQ ID NO: 18); and α 5  α 5  scYt: TGTAAACAAGGGTCCTTCAC (SEQ ID NO: 19)). Amplified products were analyzed by agarose gel electrophoresis. 
     Use of Inhibitors 
     The invention provides methods for identifying compounds which inhibit integrin activation. Integrins are surface adhesive molecules which play roles in a number of physiological processes, including activation of the immune response, inflammation, and thrombosis. Thus, the inhibitors of the invention can be used in methods to modulate the above-listed physiological processes. In addition to playing a role in the migration of normal cells, integrins are also involved in the migration and metastasis of tumor cells. Thus, the inhibitors of the invention may be useful in treating patients with tumors or cancer. 
     The inhibitors can be administered to the patient by any appropriate method suitable for the particular inhibitor, e.g., orally, intravenously, parenterally, transdermally, or transmucosally. Therapeutic doses are determined specifically for each inhibitor, most administered within the range of 0.001-100.0 mg/kg body weight, or within a range that is clinically determined as appropriate by those skilled in the art. 
     Other Embodiments 
     From the above description, one skilled in the art can easily ascertain the essential characteristics of the present invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Other embodiments are in the claims set forth below. 
     
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SEQUENCE LISTING                                                          
(1) GENERAL INFORMATION:                                                  
(iii) NUMBER OF SEQUENCES: 19                                             
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LysValGlyP hePheLysArgAsnArgProProLeuGluGluAspAsp                         
151015                                                                    
GluGluGlyGlu                                                              
20                                                                        
(2) INFORMATION FOR SEQ ID NO: 2:                                         
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(A) LENGTH: 32                                                            
 (B) TYPE: amino acid                                                     
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ArgMetGlyPhePheLysArgValArgProProGlnGluGluGlnGlu                          
151015                                                                    
 ArgGluGlnLeuGlnProHisGluAsnGlyGluGlyAsnSerGluThr                         
202530                                                                    
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(A) LENGTH: 24                                                            
(B) TYPE: amino acid                                                      
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LysLeuGlyPhePheLysArgGlnTyrLysAspMetMetSerGluGly                          
151015                                                                    
GlyProProGlyAlaGluPro Gln                                                 
20                                                                        
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(A) LENGTH: 58                                                            
(B) TYPE: amino acid                                                      
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LysValGlyPhePheLysArgAsnLeuLysGluLysMetGluA laGly                         
151015                                                                    
ArgGlyValProAsnGlyIleProAlaGluAspSerGluGlnLeuAla                          
202530                                                                    
Ser GlyGlnGluAlaGlyAspProGlyCysLeuLysProLeuHisGlu                         
354045                                                                    
LysAspSerGluSerGlyGlyGlyLysAsp                                            
5055                                                                      
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LysValAspGlyIleAspLysLeuAspIleGluPheLeuGlnProGly                          
15 1015                                                                   
GlySerThrSerSerArgGlySerTrp                                               
2025                                                                      
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(A) LENGTH: 27                                                            
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LysLeuGlyPhePheLysArgLysTyrGluLysMetThrLysAsnPro                          
151015                                                                    
AspGluIleAspGluThrThrGluLeuSe rSer                                        
2025                                                                      
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(A) LENGTH: 28                                                            
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LysLeuGlyPhePheLysArgSerLeu ProTyrGlyThrAlaMetGlu                         
151015                                                                    
LysAlaGlnLeuLysProProAlaThrSerAspAla                                      
2025                                                                      
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(A) LENGTH: 36                                                            
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LysCysGlyPhePheLysArgAsnLysLysAspHisTyrAspAlaThr                          
15 1015                                                                   
TyrHisLysAlaGluIleHisAlaGlnProSerAspLysGluArgLeu                          
202530                                                                    
ThrSerAspAla                                                              
35                                                                        
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LysLeuGlyPhePheLysArgSerArgTyrAspAspSerValProArg                          
15 1015                                                                   
TyrHisAlaValArgIleArgLysGluGluArgGluIleLysAspGlu                          
202530                                                                    
LysTyrIleAspAsnLeuGluLysLysGln TrpIleThrLysTrpAsn                         
354045                                                                    
ArgAsnGluSerTyrSer                                                        
50                                                                        
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LysLeuLeuIleThrIleHisAspArgLysGluPheAlaLysPheGlu                          
151015                                                                    
GluGluArgAlaArgAlaLysTrpAsp ThrAlaAsnAsnProLeuTyr                         
202530                                                                    
LysGluAlaThrSerThrPheThrAsnIleThrTyrArgGlyThr                             
3540 45                                                                   
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LysAlaLeuIleHisLeuSerAspLeuArgGluTyrArgArgPheGlu                          
1 51015                                                                   
LysGluLysLeuLysSerGlnTrpAsnAsnAspAsnProLeuPheLys                          
202530                                                                    
SerAlaThrThrThrV alMetAsnProLysPheAlaGluSer                               
354045                                                                    
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LysLeuLeuMetIleIleHisAspArgArgGluPheAlaLysPheGlu                          
151015                                                                    
LysGluLysMetAsnAlaLysTrpAspThrGlyGluAsnProIleT yr                         
202530                                                                    
LysSerAlaValThrThrValValAsnProLysTyrGluGlyLys                             
354045                                                                    
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GlyArgGlyAspSerPro                                                        
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GlyPhePheLysArg                                                           
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(A) LENGTH: 5                                                             
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LeuGlyPhePheLys                                                           
15                                                                        
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(A) LENGTH: 5                                                             
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ValGly PhePheLys                                                          
15                                                                        
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(A) LENGTH: 25                                                            
(B) TYPE: nucleic acid                                                    
(C) STRANDEDNESS: single                                                  
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CGGGCCTTGGAGGAGAGGGCCATTC 25                                              
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(A) LENGTH: 20                                                            
(B) TYPE: nucleic acid                                                    
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CTCTGTTGGGAGGGAAACGA20                                                    
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(A) LENGTH: 20                                                            
(B) TYPE: nucleic acid                                                    
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TGTAAACAAGGGTCCTTCAC20                                                    
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