Patent Publication Number: US-6670130-B1

Title: Oligonucleotide for detection and identification of Mycobacteria

Description:
BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The present invention relates to oligonucleotides that can be used for detection and identification of mycobacteria. More particularly, the present invention identifies the nucleotide sequence of ITS (Internal Transcribed Spacer region) of non-tuberculosis mycobacteria,  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi , and using the nucleotide sequences, it provides oligonucleotide primers or probes used for detection and identification of mycobacteria. 
     2. Description of the Related Art 
     A Even though the number of patients of tuberculosis has steadily decreased in these days, about 8 million patients have come out and about 3 million patients died of tuberculosis in a year. Moreover, in underdeveloped countries, inadequate treatment and lack of drugs for tuberculosis increase chronic carriers of drug-resistant bacteria. In 1980&#39;s, spread of AIDS has increased patients of tuberculosis even in advanced countries. In this condition, it is expected that about twelve million patents of tuberculosis would newly come out in the year 2000 (J. P. Natain, M. C. Raviglione, and A. Kochi,  Tubercle and Lung Disease , 73: 311-321, 1992; Murray C J L. And Lopez A D. The global burden of disease.  Global burden of disease and injury series. Vol.  1. Cambridge, Mass.: Harvard University Press, 1996, p349-350; Global Tuberculosis Programme: Anti-tuberculosis drug resistance, WHO Report 1997: World Health Organization. 1997). 
     In 1950&#39;s, it was reported that non-tuberculosis mycobacteria (NTM) has been able to cause diseases in human. After the report that  M. avium  complex (MAC) would bring about systemic disease in the patients of AIDS in 1980, non-tuberculosis mycobacteria have taken an interest. Diseases caused by non-tuberculosis mycobacteria are similar to tuberculosis in clinical condition and general pathological view. Non-tuberculosis mycobacteria are distributed in a wide range of living environment, and it is difficult to judge whether they have pathogenicity or not in clinical test sample. Further, since they have resistance to a number of drugs for tuberculosis, the infection is hard to treat and the recurrence rate is high. The infection of non-tuberculosis mycobacteria should be treated by other means than for tuberculosis, and therefore, accurate and fast method of detecting and identifying non-tuberculosis mycobacteria is required. The accurate and fast method of detecting and identifying both TB complex and NTM is also needed for effective treatment and management of tuberculosis. 
     Many a method has been developed to diagnose mycobacterial infection and to detect and identify mycobacteria strains. Among them, the following methods are used at present; 
     The first is a microbiological method, that is, smearing, staining and culturing test. However, this method is not suitable for mycobacteria, since they have long generation term and need long culturing time. Further, such pathogenic microorganism as mycobacteria is dangerous to infect the personnel in culture room; 
     The second is a PCR (Polymerase Chain Reaction) method. It is highly sensitive and specific to the mycobacteria and very useful to detect mycobacteria which have a long culturing time. Especially, it does not require a culturing process but uses a small amount of DNA to be amplified, therefore, only a small amount of pathogens in test sample is enough to detect and identify mycobacteria. Many a PCR process has been introduced with different target DNAs each other, and IS6110 and 16S rRNA are often used as the target (Bauer J, Andersen A B, Kremer K, and Miorner H, Usefulness of spoligotyping to discriminate IS6110 low-copy-number  Mycobacterium tuberculosis  complex strains cultured in Denmark, 1999 , J. Clin. Microbiol . 37: 2602-2606; Troesch, A., H. Nguyen, C. G. Miyada, S. Desvarenne, T. R. Gingeras, P. M. Kaplan, P. Cros and C. Mabilat. 1999, Mycobacterium species identification and rifampin resistance testing with high-density DNA probe arrays,  J. Clin. Microbiol . 37: 49-55); 
     The third is a physico-chemical process, in which lipid component in mycobacteria has been detected by HPLC, GC or mass spectrophotometer. This method is very specific but rquires expensive equipments; 
     The fourth is a method of detecting mycobacteria composition by serological method. This method uses a coagulation reaction of latex particles or blood corpuscles adsorbed with antibody to mycobacterial antigen or enzyme-linked immunological method in which enzyme is linked with antibody. It is, however, very sensitive only to be proceeded within a limited place. Further, it is difficult for this method to distinguish present infection from previous infection; 
     The next method to detect mycobacteria consists of infecting mycobacteria with mycobacteriophage L5 inserted with luciferase gene, and inspecting luminescence by luciferin in medium (W. R. Jacobs, R. G. Barletta, R. Udani, J. Chan, G. Kalkut, G. Sosne, T. Kieser, G. J. Sarkis, G. F. Hatful, and B. R. Bloom. 1993 , Science  260: 819-822); and 
     The last is a method of detecting and identifying mycobacteria by hybridization of oligonucleotide (A. Troesch, H. Nguyen, C. G. Miyada, S. Desvarenne, T. R. Gingeras, P. M. Kaplan, P. Cros and C. Mabilat. 1999 . J. Clin. Microbiol  37: 49-55). 
     Besides  Mycobacierium avium  complex (MAC) described above,  M. Fortuitum, M. chelonae  complex,  M. terrae  and  M. vaccae  are also known as non-tuberculosis mycobacteria. Among them,  M. chelonae  complex are classified into  M. chelonae  and  M. abscessus , and there is no means to distinguish one from the other at present. 
     SUMMARY OF THE INVENTION 
     To solve the problems in the prior method of detection and identification of mycobacteria, it is an objective of the present invention to provide specific oligonucleotides as probes or primers for PCR which can be used to detect mycobacteria, to distinguish TB complex from NTM, and to identify species of mycobacteria with an accuracy and effectiveness. 
     To accomplish the above objective, the present invention provides a DNA of ITS (Internal Transcribed Spacer region) of  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi  genes set forth in SEQ ID NOs: 1 to 9. 
     Further, the present invention provides, as a primer for PCR or a probe for hybridization, an oligonucleotide for detection of mycobacteria set in forth in one of SEQ ID NOs: 10 to 14; 
     an oligonucleotide for distinction of TB complex from NTB among mycobacteria set in forth in one of SEQ ID NOs: 15 to 23; 
     an oligonucleotide for detection and identification of MAC ( Mycobacterium avium  and  Mycobacterium intracellulare ) set in forth in one of SEQ ID NOs: 24 to 27; 
     an oligonucleotide for detection and identification of  Mycobacterium fortuitum  set in forth in one of SEQ ID NOs: 28 to 38; 
     an oligonucleotide for detection and identification of  Mycobacterium chelonae  set in forth in one of SEQ ID NOs: 39 to 46; 
     an oligonucleotide for detection and identification of  Mycobacterium abscessus  set in forth in one of SEQ ID NOs: 47 to 52; 
     an oligonucleotide for detection and identification of  Mycobacterium vaccae  set in forth in one of SEQ ID NOs: 53 to 64; 
     an oligonucleotide for detection and identification of  Mycobacterium flavescens  set in forth in one of SEQ ID NOs: 65 to 72; 
     an oligonucleotide for detection and identification of  Mycobacterium gordonae  set in forth in one of SEQ ID NOs: 73 to 77; 
     an oligonucleotide for detection and identification of  Mycobacterium terrae  set in forth in one of SEQ ID NOs: 78 to 100; 
     an oligonucleotide for detection and identification of  Mycobacterium scrofulaceum  set in forth in one of SEQ ID NOs: 101 to 108; 
     an oligonucleotide for detection and identification of  Mycobacterium kansasii  set in forth in one of SEQ ID NOs: 109 to 112; 
     an oligonucleotide for detection and identification of  Mycobacterium szulgai  set in forth in one of SEQ ID NOs: 113 to 116; 
     an oligonucleotide for detection and identification of  Mycobacterium marinum  and  Mycobacterium ulcerans  set in forth in one of SEQ ID NOs: 117 to 119; 
     an oligonucleotide for detection and identification of  Mycobacterium gastri  set in forth in one of SEQ ID NOs: 120 to 123; 
     an oligonucleotide for detection and identification of  Mycobacterium xenopi  set in forth in one of SEQ ID NOs: 124 to 133; 
     an oligonucleotide for detection and identification of  Mycobacterium genavense  set in forth in one of SEQ ID NOs: 134 to 141; 
     an oligonucleotide for detection and identification of  Mycobacterium malmoense  set in forth in one of SEQ ID NOs: 142 to 146; 
     an oligonucleotide for detection and identification of  Mycobacterium simiae  set in forth in one of SEQ ID NOs: 147 to 153; 
     an oligonucleotide for detection and identification of  Mycobacterium smegmatis  set in forth in one of SEQ ID NOs: 154 to 165; 
     an oligonucleotide for detection and identification of  Mycobacterium shimoidei  set in forth in one of SEQ ID NOs: 166 to 172; 
     an oligonucleotide for detection and identification of  Mycobacterium habana  set in forth in one of SEQ ID NOs: 173 to 180; 
     an oligonucleotide for detection and identification of  Mycobacterium farcinogen  set in forth in one of SEQ ID NOs: 181 to 189; 
     an oligonucleotide for detection and identification of  Mycobacterium asiaticum  set in forth in one of SEQ ID NOs: 190 to 193; 
     an oligonucleotide for detection and identification of  Mycobacterium porcinum  set in forth in one of SEQ ID NOs: 194 to 205; 
     an oligonucleotide for detection and identification of  Mycobacterium acapulcensis  set in forth in one of SEQ ID NOs: 206 to 215; 
     an oligonucleotide for detection and identification of  Mycobacterium diernhoferi  set in forth in one of SEQ ID NOs: 216 to 227; 
     an oligonucleotide for detection and identification of  Mycobacterium paratuberculosis  set in forth in one of SEQ ID NOs: 228 to 240; and 
     an oligonucleotide for detection of Mycobacteria sp. set in forth in SEQ ID NO: 241. 
     In the prior method of detecting and identifying mycobacteria using PCR, only one or two strains can be detected. According to the present invention, however, almost all of mycobacteria strains can be detected and identified, since primers and probes of the present invention have been designed form DNA sequences of ITS of mycobacteria. ITS has more polymorphic region than 16S rRNA has and ITS also has conserved region, therefore, it is highly effective as a target DNA for distinction of genotype (Gurtler, V., and V. A. Stanisich, 1996, New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region.  Microbiol . 142: 3-16). 
     The inventors identified DNA sequences of ITS of non-tuberculosis mycobacteria whose DNA had not yet been sequenced, such as  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi . Using the DNA sequences, oligonucleotides for primers or probes have been designed for detecting and identifying the mycobacteria. Further, referring to the information on DNA sequence of other mycobacteria disclosed in GenBank, and analyzing the information with multi-alignment and blast, distinctive regions of polymorphism were selected to design oligonucleotides for primers or probes to detect and identify mycobacteria. The oligonucleotides have been confirmed to detect and identify mycobacteria by specific hybridization and amplification with species-specific and genus-specific primers of PCR. 
     That is, the oligonucleotide probes of the present invention, attached to solid substrate, are hybridized only with nucleotide sequence in ITS of specific mycobacteria, and therefore, they can detect and identify the specific mycobacteria sensitively. Further, the oligonucleotide primers of identical nucleotide sequence with the above probes can also detect and identify the specific mycobacteria by amplification in PCR. Using the oligonucleotide primers or probes made from ITS of mycobacteria, it is possible to detect mycobacteria, distinguish TB complex from NTM, and identify mycobacteria species accurately and effectively. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     The above and other objects, features, and advantages of the present invention will be apparent from the following detailed description of the preferred embodiments of the invention in conjunction with the accompanying drawings, in which: 
     FIG. 1 is a schematic diagram showing ITS of mycobacteria and primers used to amplify the ITS by PCR; 
     FIG. 2 is a photograph showing the result of electrophoresis after PCR using several mycobacteria strains and a pair of primers for ITS amplification including a part of 16S rRNA and 23S rRNA of mycobacteria; 
     FIG. 3 is a photograph showing the result of electrophoresis after PCR using several mycobacteria strains and a pair of primers (ITSF and MYC2) for detecting mycobacteria; 
     FIG. 4 is a schematic diagram of multiplex PCR for detecting mycobacteria and simultaneously distinguishing TB complex from NTM; 
     FIG. 5 is a photograph showing the result of electrophoresis after multiplex PCR using a pair of primers (ITSF and MYC2) for detecting mycobacteria and a pair of primers (MTB2 and MYC2) for distinguishing TB complex from NTM; 
     FIG. 6 is a photograph showing the result of electrophoresis after PCR using each mycobacteria and each pair of species-specific primers designed from nucleotide sequence of polymorphic region of each NTM; and 
     FIG. 7 is a photograph showing the result of electrophoresis after PCR using several mycobacteria and each pair of species-specific primers designed from nucleotide sequence of polymorphic region of each NTM. 
    
    
     BEST MODE FOR CARRYING OUT THE INVENTION 
     Now, preferred embodiments of the present invention will be described with reference to the accompanying drawings. 
     EXAMPLE 1 
     Culture of Mycobacteria and Separation of Genome DNA 
     Standard strains of mycobacteria were obtained from KCTC (Korean Collection for Type Culture) and ATCC (American Type Culture Collection), clinical strains were obtained from The Korean National Tuberculosis Association, National Masan Tuberculosis Hospital and Pusan National University Hospital. The strains were stored and cultured under the management of clinical microbiology specialist in Pusan National University Hospital. The mycobacteria and pathogens used in the examples are shown in Table 8. 
     Mycobacteria DNA was extracted by the following processes: Mycobacteria were cultured on Ogawa medium. A loopful of cultured strain was put in eppendorf tube, mixed with 200 μl of InstaGene matrix (Bio-Rad Co.) and incubated at 56° C. for 30 minutes. After 10 minutes&#39; vortex mixing, the mixture was placed at 100° C. for 8 minutes. After another 10 minutes&#39; vortex mixing, the mixture was centrifuged at 12,000 rpm for 3 minutes. The supematant was moved to another tube and stored at −20° C. 2 μl of the DNA solution of each strain was used in the succeeding PCR. 
     EXAMPLE 2 
     Manufacture of Primers for Amplifying ITS of Mycobacteria 
     The ITS of mycobacteria and primers used to amplify the ITS by PCR were illustrated in FIG.  1 . The primers for amplifying ITS of mycobacteria were constructed from several section of conserved regions of 16S rRNA and 23S rRNA of mycobacteria. DNA sequences of 16S and 23S rRNA of mycobacteria disclosed in GenBank were analyzed with multialignment and blast search. The primers were designed to amplify selectively about 500 bp of ITS region with a part of 16S rRNA and 23S rRNA and have sequences set in forth in SEQ ID NOs: 242 (ITSF) and 243 (ITSR). All the primers used in Examples were manufactured by the concentration of 50 nmol with Perkin-Elmer DNA Synthesizer by BioBasic (Canada). 
     EXAMPLE 3 
     PCR and Identification of Products 
     2 μl of the DNA solution obtained in Example 1 was used in PCR. Reaction solution included: 500 mM KCl, 100 mM Tris HCl (pH 9.0), 1% Triton X-100, 0.2 mM dNTP (dATP, dGTP, dTTP and dCTP), 1.5 mM MgCl 2 , 1 pmol of primer, 1 U of Taq DNA polymerase (Bio Basic Inc.). After denaturated at 94° C. for 5 minutes, the solution was reacted 30 cycles of denaturation at 94° C. for 1 minute, annealing at 60° C. for 1 minute, and elongation at 72° C. for 1 minute. The samples were incubated further for 10 minutes at 72° C. for complete elongation. After the reaction, the PCR products were identified by electrophoresis on 1.5% agarose gel. As expected from the information of GenBank, the ITS amplified using the conserved primers of 16S rRNA and 23S rRNA was about 500 bp. 
     FIG. 2 is a photograph showing the result of electrophoresis after PCR using several mycobacteria strains and a pair of primers for ITS amplification including a part of 16S rRNA and 23S rRNA of mycobacteria. In this figure, M means molecular weight mark having intervals of 100 bp, C is a control group, lanes 1 to 9 indicate, in turn,  M. fortuitum, M. chelonae, M. intracellularae, M. avium, M. tuberculosis, M. agri, M. kansasii, M. gordonae , and  M. tuberculosis  H37Rv. It can be noted that all mycobacteria of lanes 1 to 9 have amplification of ITS gene except control group C. 
     EXAMPLE 4 
     Determination of DNA Sequence of Mycobacteria ITS 
     After the identification of PCR products, the reactants of  M. fortuitum, M. chelonae, M. abscessus, M. vaccae, M. flavescens, M. asiaticum, M. porcinum, M. acapulcensis and M. diernhoferi , whose DNA sequences of ITS have not yet been determined, were amplified by PCR. The PCR products were used directly in determining DNA sequence of ITS. 
     DNA was used by the concentration of 200 μmol. DNA sequence was determined by dye terminator method using universal primer M13 with DNA auto sequencer (Perkin-Elmer, ABI prim 377 sequencer). 
     SEQ ID NOs 1 to 9 indicate DNA sequences of ITS of  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacierium abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi , in turn, 
     EXAMPLE 5 
     Design and Synthesis of Oligonucleotide Primer 
     Each primer of about 20 bp was designed from DNA sequences of ITS of  Mycobacterium fortuitum, Mycobacterium cheloniae, Mycobacierizim abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi , obtained in Example 4, and compared with DNA sequences of ITS of other mycobacteria obtained from GenBank by multialignment and blast. 
     Based on the result, conservative DNA sequences of mycobacteria ITS were designed as primers or probes for detection of mycobacteria, and DNA sequences of high polymorphism were designed as species-specific primers or probes. Tables 1 to 7 depict the designed primers or probes with their position and SEQ ID Nos. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 Target Strain 
                 Probe Name 
                 Position in ITS 
                 SEQ ID NO. 
               
               
                   
               
             
            
               
                 Mycobacteria 
                 MYC1 
                 Variabie to the 
                 10 
               
               
                   
                 MYC2 
                 species 
                 11 
               
               
                   
                 MYC3 
                   
                 12 
               
               
                   
                 MYC4 
                   
                 13 
               
               
                   
                 MYC5 
                   
                 14 
               
               
                 TB complex 
                 MTB1 
                 166-185 
                 15 
               
               
                   
                 MTB2 
                 65-84 
                 16 
               
               
                   
                 MTB3 
                 20-39 
                 17 
               
               
                   
                 MTB4 
                 41-60 
                 18 
               
               
                   
                 MTB5 
                 60-79 
                 19 
               
               
                   
                 MTB6 
                  81-100 
                 20 
               
               
                   
                 MTB7 
                 125-144 
                 21 
               
               
                   
                 MTB8 
                 139-158 
                 22 
               
               
                   
                 MTB9 
                 203-222 
                 23 
               
               
                   M. avium  -  M.   
                 MAC1 
                 241-260 
                 24 
               
               
                 
                   intracellularae 
                 
                 MAC2 
                 142-161 
                 25 
               
               
                 (MAC) 
                 MAC3 
                  92-111 
                 26 
               
               
                   
                 MAC4 
                 117-136 
                 27 
               
               
                 
                   M. fortuitum 
                 
                 FOR1 
                 40-59 
                 28 
               
               
                   
                 FOR2 
                 44-63 
                 29 
               
               
                   
                 FOR3 
                 64-83 
                 30 
               
               
                   
                 FOR4 
                 78-97 
                 31 
               
               
                   
                 FOR5 
                  89-108 
                 32 
               
               
                   
                 FOR6 
                 109-128 
                 33 
               
               
                   
                 FOR7 
                 114-133 
                 34 
               
               
                   
                 FOR8 
                 134-153 
                 35 
               
               
                   
                 FOR9 
                 157-176 
                 36 
               
               
                   
                 FOR10 
                 246-265 
                 37 
               
               
                   
                 FOR11 
                 289-308 
                 38 
               
               
                 
                   M. chelonae 
                 
                 CHE1 
                 11-30 
                 39 
               
               
                   
                 CHE2 
                 29-48 
                 40 
               
               
                   
                 CHE3 
                 58-77 
                 41 
               
               
                   
                 CHE4 
                 78-97 
                 42 
               
               
                   
                 CHE5 
                 109-128 
                 43 
               
               
                   
                 CHE6 
                 132-151 
                 44 
               
               
                   
                 CHE7 
                 171-190 
                 45 
               
               
                   
                 CHE8 
                 246-265 
                 46 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
               
                 Target Strain 
                 Probe Name 
                 Position in ITS 
                 SEQ ID NO. 
               
               
                   
               
             
            
               
                 
                   M. abscessus 
                 
                 ABC1 
                 37-56 
                 47 
               
               
                   
                 ABC2 
                 55-74 
                 48 
               
               
                   
                 ABC3 
                 247-266 
                 49 
               
               
                   
                 ABC4 
                 263-282 
                 50 
               
               
                   
                 ABC5 
                 270-289 
                 51 
               
               
                   
                 ABC6 
                 261-280 
                 52 
               
               
                 
                   M. vaccae 
                 
                 VAC1 
                 18-37 
                 53 
               
               
                   
                 VAC2 
                 38-57 
                 54 
               
               
                   
                 VAC3 
                 58-77 
                 55 
               
               
                   
                 VAC4 
                 118-137 
                 56 
               
               
                   
                 VAC5 
                 138-157 
                 57 
               
               
                   
                 VAC6 
                 158-177 
                 58 
               
               
                   
                 VAC7 
                 178-197 
                 59 
               
               
                   
                 VAC8 
                 199-218 
                 60 
               
               
                   
                 VAC9 
                 219-238 
                 61 
               
               
                   
                 VAC10 
                 265-284 
                 62 
               
               
                   
                 VAC11 
                 298-317 
                 63 
               
               
                   
                 VAC12 
                 321-340 
                 64 
               
               
                 
                   M. flavescens 
                 
                 FLA1 
                 12-31 
                 65 
               
               
                   
                 FLA2 
                 32-51 
                 66 
               
               
                   
                 FLA3 
                 52-71 
                 67 
               
               
                   
                 FLA4 
                 72-91 
                 68 
               
               
                   
                 FLA5 
                 105-124 
                 69 
               
               
                   
                 FLA6 
                 125-144 
                 70 
               
               
                   
                 FLA7 
                 173-192 
                 71 
               
               
                   
                 FLA8 
                 278-297 
                 72 
               
               
                 
                   M. gordonae 
                 
                 GOR1 
                  84-103 
                 73 
               
               
                   
                 GOR2 
                 249-268 
                 74 
               
               
                   
                 GOR3 
                 216-235 
                 75 
               
               
                   
                 GOR4 
                 201-220 
                 76 
               
               
                   
                 GOR5 
                 223-242 
                 77 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                 TABLE 3 
               
               
                   
               
               
                 Target Strain 
                 Probe Name 
                 Position in ITS 
                 SEQ ID NO. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 
                   M. terrae 
                 
                 TER1 
                 178-197 
                 78 
               
               
                   
                 TER2 
                 237-256 
                 79 
               
               
                   
                 TER3 
                 24-43 
                 80 
               
               
                   
                 TER4 
                 70-89 
                 81 
               
               
                   
                 TER5 
                  89-108 
                 82 
               
               
                   
                 TER6 
                 102-121 
                 83 
               
               
                   
                 TER7 
                 122-141 
                 84 
               
               
                   
                 TER8 
                 142-161 
                 85 
               
               
                   
                 TER9 
                 162-181 
                 86 
               
               
                   
                 TER10 
                 182-201 
                 87 
               
               
                   
                 TER11 
                 202-221 
                 88 
               
               
                   
                 TER12 
                 222-241 
                 89 
               
               
                   
                 TER13 
                 238-257 
                 90 
               
               
                   
                 TER14 
                 307-326 
                 91 
               
               
                   
                 TER15 
                 322-341 
                 92 
               
               
                   
                 TER16 
                 342-361 
                 93 
               
               
                   
                 TER17 
                 362-381 
                 94 
               
               
                   
                 TER18 
                 382-401 
                 95 
               
               
                   
                 TER19 
                 12-21 
                 96 
               
               
                   
                 TER20 
                 31-50 
                 97 
               
               
                   
                 TER21 
                 52-71 
                 98 
               
               
                   
                 TER22 
                 72-91 
                 99 
               
               
                   
                 TER23 
                  82-101 
                 100 
               
               
                 
                   M. scrofulaceum 
                 
                 SCO1 
                 118-137 
                 101 
               
               
                   
                 SCO2 
                 131-150 
                 102 
               
               
                   
                 SCO3 
                 210-229 
                 103 
               
               
                   
                 SCO4 
                  84-103 
                 104 
               
               
                   
                 SCO5 
                 152-171 
                 105 
               
               
                   
                 SCO6 
                 200-219 
                 106 
               
               
                   
                 SCO7 
                 221-240 
                 107 
               
               
                   
                 SCO8 
                 241-260 
                 108 
               
               
                 M. kansasii 
                 KAN1 
                 35-54 
                 109 
               
               
                   
                 KAN2 
                 238-257 
                 110 
               
               
                   
                 KAN3 
                  83-102 
                 111 
               
               
                   
                 KAN4 
                 214-233 
                 112 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                 TABLE 4 
               
               
                   
               
               
                 Target Strain 
                 Probe Name 
                 Position in ITS 
                 SEQ ID NO. 
               
               
                   
               
             
            
               
                 
                   M. szulgai 
                 
                 SZU1 
                 124-143 
                 113 
               
               
                   
                 SZU2 
                 209-228 
                 114 
               
               
                   
                 SZU3 
                 227-246 
                 115 
               
               
                   
                 SZU4 
                 247-166 
                 116 
               
               
                   M. marinum  and 
                 MAR-ULC1 
                  85-104 
                 117 
               
               
                 
                   M. ulcerans 
                 
                 MAR-ULC2 
                 128-147 
                 118 
               
               
                   
                 MAR-ULC3 
                 224-243 
                 119 
               
               
                 
                   M. gastri 
                 
                 GAS1 
                  85-104 
                 120 
               
               
                   
                 GAS2 
                 145-164 
                 121 
               
               
                   
                 GAS3 
                 133-152 
                 122 
               
               
                   
                 GAS4 
                 239-258 
                 123 
               
               
                 
                   M. xenopi 
                 
                 XEN1 
                 190-209 
                 124 
               
               
                   
                 XEN2 
                  1-20 
                 125 
               
               
                   
                 XEN3 
                 21-40 
                 126 
               
               
                   
                 XEN4 
                 41-60 
                 127 
               
               
                   
                 XEN5 
                 61-80 
                 128 
               
               
                   
                 XEN6 
                  81-100 
                 129 
               
               
                   
                 XEN7 
                 121-140 
                 130 
               
               
                   
                 XEN8 
                 141-160 
                 131 
               
               
                   
                 XEN9 
                 201-220 
                 132 
               
               
                   
                 XEN10 
                 221-240 
                 133 
               
               
                 
                   M. genavense 
                 
                 GEN1 
                 190-209 
                 134 
               
               
                   
                 GEN2 
                  85-104 
                 135 
               
               
                   
                 GEN3 
                 131-150 
                 136 
               
               
                   
                 GEN4 
                 147-166 
                 137 
               
               
                   
                 GEN5 
                 186-205 
                 138 
               
               
                   
                 GEN6 
                 206-225 
                 139 
               
               
                   
                 GEN7 
                 226-245 
                 140 
               
               
                   
                 GEN8 
                 240-265 
                 141 
               
               
                 
                   M. malmoense 
                 
                 MAL1 
                 203-222 
                 142 
               
               
                   
                 MAL2 
                 29-48 
                 143 
               
               
                   
                 MAL3 
                 136-155 
                 144 
               
               
                   
                 MAL4 
                 222-241 
                 145 
               
               
                   
                 MAL5 
                 242-261 
                 146 
               
               
                 
                   M. simiae 
                 
                 SIM1 
                  83-102 
                 147 
               
               
                   
                 SIM2 
                 129-148 
                 148 
               
               
                   
                 SIM3 
                 208-227 
                 149 
               
               
                   
                 SIM4 
                 22-41 
                 150 
               
               
                   
                 SIM5 
                 80-99 
                 151 
               
               
                   
                 SIM6 
                 136-155 
                 152 
               
               
                   
                 SIM7 
                 241-260 
                 153 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                 TABLE 5 
               
               
                   
               
               
                 Target Strain 
                 Probe Name 
                 Position in ITS 
                 SEQ ID NO. 
               
               
                   
               
             
            
               
                 
                   M. smegmatis 
                 
                 SMEG1 
                 17-36 
                 154 
               
               
                   
                 SMEG2 
                 37-56 
                 155 
               
               
                   
                 SMEG3 
                 57-76 
                 156 
               
               
                   
                 SMEG4 
                 77-96 
                 157 
               
               
                   
                 SMEG5 
                  97-116 
                 158 
               
               
                   
                 SMEG6 
                 117-136 
                 159 
               
               
                   
                 SMEG7 
                 137-156 
                 160 
               
               
                   
                 SMEG8 
                 157-176 
                 161 
               
               
                   
                 SMEG9 
                 177-196 
                 162 
               
               
                   
                 SMEG10 
                 193-212 
                 163 
               
               
                   
                 SMEG11 
                 61-80 
                 164 
               
               
                   
                 SMEG12 
                 112-131 
                 165 
               
               
                 
                   M. shimoidei 
                 
                 SHI1 
                  89-108 
                 166 
               
               
                   
                 SHI2 
                 20-39 
                 167 
               
               
                   
                 SHI3 
                 70-89 
                 168 
               
               
                   
                 SH14 
                  97-116 
                 169 
               
               
                   
                 SHI5 
                 135-154 
                 170 
               
               
                   
                 SHI6 
                 224-243 
                 171 
               
               
                   
                 SHI7 
                 244-263 
                 172 
               
               
                 
                   M. habana 
                 
                 HAB1 
                  86-105 
                 173 
               
               
                   
                 HAB2 
                 17-36 
                 174 
               
               
                   
                 HAB3 
                 51-70 
                 175 
               
               
                   
                 HAB4 
                  81-100 
                 176 
               
               
                   
                 HAB5 
                 134-153 
                 177 
               
               
                   
                 HAB6 
                 175-194 
                 178 
               
               
                   
                 HAB7 
                 200-219 
                 179 
               
               
                   
                 HAB8 
                 242-261 
                 180 
               
               
                 
                   M. farcinogen 
                 
                 FAR1 
                 122-141 
                 181 
               
               
                   
                 FAR2 
                 111-130 
                 182 
               
               
                   
                 FAR3 
                 22-41 
                 183 
               
               
                   
                 FAR4 
                 48-67 
                 184 
               
               
                   
                 FAR5 
                 76-95 
                 185 
               
               
                   
                 FAR6 
                 108-127 
                 186 
               
               
                   
                 FAR7 
                 114-133 
                 187 
               
               
                   
                 FAR8 
                 275-294 
                 188 
               
               
                   
                 FAR9 
                 295-314 
                 189 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                 TABLE 6 
               
               
                   
               
               
                 Target Strain 
                 Probe Name 
                 Position in ITS 
                 SEQ ID NO. 
               
               
                   
               
             
            
               
                 
                   M. asiaticum 
                 
                 ASI1 
                  82-101 
                 190 
               
               
                   
                 ASI2 
                 145-164 
                 191 
               
               
                   
                 ASI3 
                 189-208 
                 192 
               
               
                   
                 ASI4 
                 274-293 
                 193 
               
               
                 
                   M. porcinum 
                 
                 POR1 
                 45-64 
                 194 
               
               
                   
                 POR2 
                 13-32 
                 195 
               
               
                   
                 POR3 
                 67-86 
                 196 
               
               
                   
                 POR4 
                  91-110 
                 197 
               
               
                   
                 POR5 
                 115-134 
                 198 
               
               
                   
                 POR6 
                 137-156 
                 199 
               
               
                   
                 POR7 
                 164-183 
                 200 
               
               
                   
                 POR8 
                 194-213 
                 201 
               
               
                   
                 POR9 
                 221-240 
                 202 
               
               
                   
                 POR10 
                 273-292 
                 203 
               
               
                   
                 POR11 
                 298-317 
                 204 
               
               
                   
                 POR12 
                 325-344 
                 205 
               
               
                 
                   M. acapulcensis 
                 
                 ACA1 
                 66-85 
                 206 
               
               
                   
                 ACA2 
                 112-131 
                 207 
               
               
                   
                 ACA3 
                 132-151 
                 208 
               
               
                   
                 ACA4 
                 178-197 
                 209 
               
               
                   
                 ACA5 
                 198-217 
                 210 
               
               
                   
                 ACA6 
                 219-238 
                 211 
               
               
                   
                 ACA7 
                 242-261 
                 212 
               
               
                   
                 ACA8 
                 262-281 
                 213 
               
               
                   
                 ACA9 
                 318-337 
                 214 
               
               
                   
                 ACA10 
                 350-369 
                 215 
               
               
                 
                   M. diernhoferi 
                 
                 DIE1 
                 16-35 
                 216 
               
               
                   
                 DIE2 
                 36-55 
                 217 
               
               
                   
                 DIE3 
                 62-81 
                 218 
               
               
                   
                 DIE4 
                 103-122 
                 219 
               
               
                   
                 DIE5 
                 154-173 
                 220 
               
               
                   
                 DIE6 
                 175-194 
                 221 
               
               
                   
                 DIE7 
                 195-214 
                 222 
               
               
                   
                 DIE8 
                 232-251 
                 223 
               
               
                   
                 DIE9 
                 261-280 
                 224 
               
               
                   
                 DIE10 
                 282-301 
                 225 
               
               
                   
                 DIE11 
                 304-323 
                 226 
               
               
                   
                 DIE12 
                 344-363 
                 227 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
             
               
                   
                 TABLE 7 
               
               
                   
                   
               
               
                   
                 Target Strain 
                 Probe Name 
                 Position in ITS 
                 SEQ ID NO. 
               
               
                   
                   
               
             
            
               
                   
                 
                   M. para- 
                 
                 PARA1 
                  7-26 
                 228 
               
               
                   
                 
                   tuberculosis 
                 
                 PARA2 
                 30-49 
                 229 
               
               
                   
                   
                 PARA3 
                 40-59 
                 230 
               
               
                   
                   
                 PARA4 
                 50-69 
                 231 
               
               
                   
                   
                 PARA5 
                 71-90 
                 232 
               
               
                   
                   
                 PARA6 
                  83-102 
                 233 
               
               
                   
                   
                 PARA7 
                 103-122 
                 234 
               
               
                   
                   
                 PARA8 
                 135-154 
                 235 
               
               
                   
                   
                 PARA9 
                 157-176 
                 236 
               
               
                   
                   
                 PARA10 
                 178-197 
                 237 
               
               
                   
                   
                 PARA11 
                 198-217 
                 238 
               
               
                   
                   
                 PARA12 
                 219-238 
                 239 
               
               
                   
                   
                 PARA13 
                 241-260 
                 240 
               
               
                   
                 M. sp 
                 SP1 
                 225-244 
                 241 
               
               
                   
                   
               
            
           
         
       
     
     EXAMPLE 6 
     Result of PCR Using Primers for Detecting Mycobacteria 
     Genus-specific primers, designed from conserved DNA sequence in mycobacteria were used for detecting mycobacteria. Among the primers manufactured from the ITS sequence of 270-350 bp, a pair of primers, ITSF (SEQ ID NO. 242) and MYC2 (SEQ ID NO. 11) were used to proceed PCR. As a result, amplified nucleotides of about 350 bp were obtained in mycobacteria strains, while no amplification was occurred in  Staphylococcus aureus, Enterococcus faecium  and  Serratia marcescens . Therefore, it is understood that the primers could be used for detecting mycobacteria. 
     FIG. 3 is a photograph showing the result of electrophoresis after PCR using several mycobacteria strains and a pair of primers (ITSF and MYC2) for detecting mycobacteria. In this figure, M indicates a size marker of 100 bp ladder; C indicates a negative control; lane 1 indicates  M. abscessus  ATCC 19977; lane 2 , M. agri  ATCC 27406; lane 3 , M. asiaticum  ATCC 25276; lane 4 , M. austroafricanum  ATCC 33464; lane 5 , M. avium  ATCC 25291; lane 6 , M. bovis  ATCC 19210; lane 7 , M. chelonae  ATCC 35752; lane 8 , M. flavescens  ATCC 14474; lane 9 , M. fortuitum  ATCC 6841; lane 10 , M. gordonae  ATCC 14470; lane 11 , M. intracellularae  ATCC 13950; lane 12 , M. kansasii  ATCC 12478; lane 13 , M. phlei  ATCC 354; lane 14 , M. scrofulaceutm  ATCC 19981; lane 15 , M. smegmatis  ATCC 21701; lane 16 , M. szulgai  ATCC 35799; lane 17 , M. terrae  ATCC 15755; lane 18 , M. triviale  ATCC 23292; lane 19 , M. tuberculosis  H37Rv; and lane 20 , M. vaccae  ATCC 15483. It can be seen that all mycobacteria of lanes 1 to 20 show ITS amplification except negative control C. 
     Table 8 shows the result of PCR using a pair of primers (ITSF and MYC2) for detecting mycobacteria. In this table, + indicates amplification occurred and − indicates no amplification. It can be seen that no amplification has occurred in other strains than mycobacteria. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 8 
               
               
                   
               
               
                 Name of strain 
                 result 
                 Name of strain 
                 result 
               
               
                   
               
             
            
               
                   M. tuberculosis  H37Rv 
                 + 
                   M. phlei  ATCC 354 
                 + 
               
               
                   M. bovis  ATCC 19210 
                 + 
                 
                   Aeromonas hydrophila 
                 
                 − 
               
               
                   M. avium  ATCC 25291 
                 + 
                 
                   Burkholderia cepacia 
                 
                 − 
               
               
                   M. intracellulare  ATCC 
                 + 
                 
                   Candida albicans 
                 
                 − 
               
               
                 13950 
               
               
                   M. abscessus  ATCC 19977 
                 + 
                 
                   Citrobacter freundii 
                 
                 − 
               
               
                   M. chelonae  ATCC 35752 
                 + 
                 
                   Enterobacter aerogenes 
                 
                 − 
               
               
                   M. flavescens  ATCC 14474 
                 + 
                 
                   Enterobacter cloacae 
                 
                 − 
               
               
                   M. fortuitum  ATCC 6841 
                 + 
                 
                   Enterococcus faecalis 
                 
                 − 
               
               
                   M. gastri  ATCC 15754 
                 + 
                 
                   Enterococcus faecium 
                 
                 − 
               
               
                   M. genavense  ATCC 51233 
                 + 
                 
                   Enterococcus raffinosis 
                 
                 − 
               
               
                   M. gordonae  ATCC 14470 
                 + 
                 
                   Escherichia coli 
                 
                 − 
               
               
                   M. kansasii  ATCC 12478 
                 + 
                 
                   Klebsiella pneumoniae 
                 
                 − 
               
               
                   M. malmoense  ATCC 29571 
                 + 
                 
                   Plesiomonas shigelloides 
                 
                 − 
               
               
                   M. scrofulaceum  ATCC 
                 + 
                 
                   Proteus mirabilis 
                 
                 − 
               
               
                 19981 
               
               
                   M. simiae  ATCC 25275 
                 + 
                 
                   Proteus vulgaris 
                 
                 − 
               
               
                   M. smegmatis  ATCC 21701 
                 + 
                 
                   Providencia rettgeri 
                 
                 − 
               
               
                   M. szulgai  ATCC 35799 
                 + 
                 
                   Pseudomonas aeruginosa 
                 
                 − 
               
               
                   M. terrae  ATCC 15755 
                 + 
                 
                   Rahnella aquatilis 
                 
                 − 
               
               
                   M. vaccae  ATCC 15483 
                 + 
                 Salmonella spp. 
                 − 
               
               
                   M. xenopi  ATCC 19250 
                 + 
                 
                   Serratia marcescens 
                 
                 − 
               
               
                   M. marinum  ATCC 927 
                 + 
                 
                   Shewanella putrefaciens 
                 
                 − 
               
               
                   M. ulcerance  ATCC 19423 
                 + 
                 
                   Shigella flexneri 
                 
                 − 
               
               
                   M. porcinum  ATCC 33776 
                 + 
                 
                   Shigella sonnei 
                 
                 − 
               
               
                   M. asiaticum  ATCC 25276 
                 + 
                 
                   Staphylococcus epidermidis 
                 
                 − 
               
               
                   M. acapulcensis  ATCC 14473 
                 + 
                 
                   Staphylococcus aureus 
                 
                 − 
               
               
                   M. diernhoferi  ATCC 19340 
                 + 
                 
                   Streptococcus agalactiae 
                 
                 − 
               
               
                   M. agri  ATCC 27406 
                 + 
                 
                   Streptococcus intermidius 
                 
                 − 
               
               
                   M. austroafricanum  ATCC 
                 + 
                 
                   Streptococcus pneumoniae 
                 
                 − 
               
               
                 33464 
               
               
                   M. triviale  ATCC 23292 
                 + 
                 
                   Vibrio parahemolyticus 
                 
                 − 
               
               
                   
               
            
           
         
       
     
     EXAMPLE 7 
     Result of PCR Using Primers for TB Complex 
     Test for identifying each strain using the oligonucleotide primers manufactured in the previous Examples was confirmed by amplification in PCR. For identifying TB complex, multiplex PCR was carried using a pair of primers (ITSF and MYC2) for detecting mycobacteria and a pair of primers (MTB2 and MYC2; SEQ ID Nos. 16 and 11) for distinguishing TB complex from NTM. 
     FIG. 4 is a schematic diagram of multiplex PCR for detecting mycobacteria and simultaneously distinguishing TB complex from NTM. 
     FIG. 5 is a photograph showing the result of electrophoresis after multiplex PCR using a pair of primers (ITSF and MYC2) for detecting mycobacteria and a pair of primers (MTB2 and MYC2) for distinguishing TB complex from NTM. In this figure, M indicates a size marker of 100 bp ladder; C indicates a negative control; lanes 1 and 2 indicate TB complex,  M. tuberculosis  H37Rv and  M. bovis , respectively; and lanes 3 to 10 indicate  M. avium, M. intracellularae, M. fortuitum, M. chelonae, M. gordonae, M. szulgai, M. terrae  and  M. scrofulaceum  ATCC 19981, in turn. In lanes 1 and 2 of TB complex, two bands are formed by double amplification due to primers for detecting mycobacteria and primers for TB complex, while in the other lanes of NTM strains, only one band is formed, which confirms single amplification. 
     EXAMPLE 8 
     Result of PCR Using Primers for Identifying NTM Strains 
     PCR was carried out using species-specific primers manufactured from DNA sequence of polymorphic site of each strain for identifying NTM species. 
     Specifically, after selecting SEQ ID NOs. 16 and 21 for  M. tuberculosis  H37Rv and  M. bovis , SEQ ID NOs. 24 and 27 for  M. avium  and  M. intracellularae , SEQ ID NOs. 29 and 37 for  M. fortuitum , SEQ ID NOs. 41 and 44 for  M. chelonae , SEQ ID NOs. 48 and 49 for  M. abscessus , SEQ ID NOs. 55 and 63 for  M. vaccae , SEQ ID NOs. 66 and 72 for  M. flavescens , SEQ ID NOs. 73 and 75 for  M. gordonae , SEQ ID NOs. 88 and 96 for  M. terrae , SEQ ID NOs. 102 and 103 for  M. scrofulaceum , SEQ ID NOs. 109 and 110 for  M. kansasii , SEQ ID NOs. 113 and 114 for  M. szulgai , SEQ ID NOs. 117 and 119 for  M. marinum  and  M. ulcerans , SEQ ID NOs. 120 and 123 for  M. gastri , SEQ ID NOs. 128 and 132 for  M. xenopi , SEQ ID NOs. 135 and 141 for  M. genavense , SEQ ID NOs. 143 and 145 for  M. malmoense , SEQ ID NOs. 147 and 149 for  M. simiae , and SEQ ID NOs. 154 and 159 for  M. smegmalis , each mycobacteria was carried out PCR using each pair of primers of which the first has been sense strand and the second has been antisense strand. After the reaction, each resultant was treated by electrophoresis. 
     FIG. 6 is a photograph showing the result of electrophoresis after PCR using each mycobacteria and each pair of species-specific primers designed from nucleotide sequence of polymorphic region of each NTM. In this figure, M indicates a size marker of 100 bp ladder; C indicates a negative control; lanes 1 and 2 indicate TB complex,  M. tuberculosis  H37Rv and  M. bovis  ATCC 19210, respectively; lanes 3 and 4 indicate  M. avium  ATCC 25291 and  M. intracellularae  ATCC 13950, respectively; lane 5 indicates  M. fortuitum  ATCC 6841; lane 6 , M. chelonae  ATCC 35752; lane 7 , M. abscessus  ATCC 19977; lane 8 , M. vaccae  ATCC 15483; lane 9 , M. flavescens  ATCC 14474; lane 10 , M. gordonae  ATCC 14470; lane 11 , M. terrae  ATCC 15755; lane 12 , M. scrofulaceum  ATCC 19981; lane 13 , M. kansasii  ATCC 12478; lane 14 , M. szulgai  ATCC 35799; lane 15 , M. marinum  ATCC 927; lane 16 , M. ulcerans  ATCC 19423; lane 17 , M. gastri  ATCC 15754; lane 18 , M. xenopi  ATCC 19250; lane 19 , M. genavense  ATCC 1233; lane 20 , M. malmoense  ATCC 29571; lane 21 , M. simiae  ATCC 25275; and lane 22 indicates  M. smegmatis  ATCC 21701. Species-specific amplifications can be seen in lanes 1 to 22. 
     FIG. 7 is a photograph showing the result of electrophoresis after PCR using several mycobacteria and each pair of species-specific primers designed from nucleotide sequence of polymorphic region of each NTM. In this figure, the first photograph using the pair of primers (ITSF and MYC2) specific for TB complex shows amplifications in lanes 1 and 2 of  M. tuberculosis  H37Rv and  M. bovis ; the second using the pair of primers (MAC1 and MAC4) specific for  M. avium  and  M. intracellularae  shows amplifications in lanes 3 and 4 of  M. avium  and  M. intracellularae ; the third using the pair of primers (FOR2 and FOR10) specific for  M. fortuitum  shows amplification in lane 5 of  M. fortuitum ; the fourth using the pair of primers (CHE3 and CHE6) specific for  M. chelotiae  shows amplification in lane 6 of  M. chelonae ; the fifth using the pair of primers (GOR1 and GOR2) specific for  M. gordonae  shows amplification in lane 7 of  M. gordonae; the sixth using the pair of primers (SZU 1 and SZU2) specific for  M. szulgai  shows amplification in lane 8 of  M. szulgai; and the seventh using the pair of primers (SCO 1 and SCO2) specific for  M. scrofulaceum  shows amplification in lane 10 of  M. scrofulaceum . Lane 9 indicates  M. terrae , which shows no amplification with the above species—specific primer pairs. 
     Therefore, PCR using each pair of species-specific primers can detect and identify specifically each species of mycobacteria. 
     INDUSTRIAL APPLICABILITY 
     As described above, identifying DNA sequences of ITS (Internal Transcribed Spacer region) of non-tuberculosis mycobacteria,  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinur, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi , and using the DNA sequences, oligonucleotide primers or probes can been designed for detecting and identifying mycobacteria. Using the primers for PCR or probes for hybridization, it is possible to detect mycobacteria, distinguish TB complex from NTM. and identify mycobacteria species with rapidity and effectiveness. Such detection and identification method makes the diagnosis of complex infection effective, and therefore, it is possible to treat accurately mycobacterial infection including tuberculosis. 
     While this invention has been described in connection with what is presently considered to be the most practical and preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiment, but, on the contrary, it is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. 
     
       
         
           
               
            
               
                   
               
               
                                                SEQUENCE LISTING 
               
               
                   
               
               
                   
               
               
                 &lt;160&gt; NUMBER OF SEQ ID NOS: 243 
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 1 
               
               
                 &lt;211&gt; LENGTH: 386 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 1 
               
               
                   
               
               
                 aggagcacca cgaaaagggt tgagacactg ggtcttaccc gagccgtgag gaaccggttg     60 
               
               
                   
               
               
                 cctgtagtgg gcacggtttg gtgcacaaca aacttttttg actgccagac acactattgg    120 
               
               
                   
               
               
                 gctttgagac aacaggcccg tgcccctttt ggggggtggc atccggttgc gggtgtcggc    180 
               
               
                   
               
               
                 gtgttgttgc ctcactttgg tggtggggtg tggtgtttga tttgtggata gtggttgcga    240 
               
               
                   
               
               
                 gcatctagca cgcagaatcg tgtggtctca ctccttgtgg gtggggctgg ttttgtgtgt    300 
               
               
                   
               
               
                 tgatgtgcaa tttcttttga aactcatttt tggtttttgt gttgtaagtg tttaagggcg    360 
               
               
                   
               
               
                 catggtggat gccttggcag gatcca                                         386 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 2 
               
               
                 &lt;211&gt; LENGTH: 338 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium chelonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 2 
               
               
                   
               
               
                 aggagcacca tttcccagcc gaatgagctt gggaacataa agcgagtttc tgtagtggtt     60 
               
               
                   
               
               
                 actcgcttgg tgaatatgtt ttataaatcc tgtccacccc gtggataggt agtcggcaaa    120 
               
               
                   
               
               
                 acgtcggact gtcaatagaa ttgaaacgct ggcacactgt tgggtcctga ggcaacacat    180 
               
               
                   
               
               
                 tgtgttgtca ccctgcttgg tggtggggtg tggtctttga cttatggata gtggttgcga    240 
               
               
                   
               
               
                 gcatctaaca aacctcgctc gtttacgagt gaggttagtt tttgcaattt attagctaag    300 
               
               
                   
               
               
                 ttcttaaggg cacatggtgg atgccttggc aggatcca                            338 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 3 
               
               
                 &lt;211&gt; LENGTH: 317 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium abscessus 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 3 
               
               
                   
               
               
                 aaggagcacc atttcccagt cgaatgaact agggaacata aagtaggcat ctgtagtgga     60 
               
               
                   
               
               
                 tatctacttg gtgaatatgt tttgtaaatc ctgtccaccc cgtggatggg tagtcggcaa    120 
               
               
                   
               
               
                 aacgtcggac tgtcataaga attgaaacgc tggcacactg ttgggtcccg aggcaacacg    180 
               
               
                   
               
               
                 ttgtgttgtc accctgcttg gtggtggggt gtggactttg acttctgaat agtggttgcg    240 
               
               
                   
               
               
                 agcatctaaa catagcctcg ctcgttttcg agtggggctg gtttttgcaa ttttattagc    300 
               
               
                   
               
               
                 taagttctta agggcgc                                                   317 
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 4 
               
               
                 &lt;211&gt; LENGTH: 429 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 4 
               
               
                   
               
               
                 aaggagcacc acgagaatca ggcccgccca catcgtgtgg gggttcggtg atctgatcga     60 
               
               
                   
               
               
                 ttcgttggat ggcctttcat ctgtagtgga tgggggtctg gtgcacatga caaacttggc    120 
               
               
                   
               
               
                 cgagccggta gggaatgccg gcgagggaaa tcatcagaca cactattggg ctttgagaca    180 
               
               
                   
               
               
                 acaagcccgt gccccttttt ttgggggtgg ctctgcgttg gcagggtcgg cgtgttgttg    240 
               
               
                   
               
               
                 ccccgctttg gtggtggggt gtggtgtttg attcgtggat agtggttgcg agcatctgaa    300 
               
               
                   
               
               
                 tgcacagcgc ttgtggtgtt gtgtgttcgg tgtaatgcaa atttttctga tactcgcatg    360 
               
               
                   
               
               
                 cagtcccttt ttgggggtgt gtgtgggtga ctcatttttt ggttttgtgt tgtaagtgtt    420 
               
               
                   
               
               
                 taagggcgc                                                            429 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 5 
               
               
                 &lt;211&gt; LENGTH: 379 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium flavescens 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 5 
               
               
                   
               
               
                 aaggagcacc atttattgtt cccccgtccc cacgtgtgtg ggatcagtgc ggttgggaga     60 
               
               
                   
               
               
                 tcagtgccgg gcctgtagtg ggtttccggt gggtgcacaa caaacgtgag aagtggtgtg    120 
               
               
                   
               
               
                 ggaacactgc tttgaggaat catcagacac actattgggc tttgaggcaa caggcccgt     180 
               
               
                   
               
               
                 gtttccctgg ccactgtgtg tggtgggggg tctggtgtcg ccctgtcttt ggtggtgggg    240 
               
               
                   
               
               
                 tgtggtgttt gattcgtgga tagtggttgc gagcatctga acaggtggct cccttttggg    300 
               
               
                   
               
               
                 ggttgcttgt tttgataatg caatttttta ttcttccgag aatatttttc tgttttgtgt    360 
               
               
                   
               
               
                 tgtaagtgtt taagggcgc                                                 379 
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 6 
               
               
                 &lt;211&gt; LENGTH: 364 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium asiaticum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 6 
               
               
                   
               
               
                 tggatccgac gaagtcgtaa caaggtagcc gtaccggaag gtgcggctgg atcacctcct     60 
               
               
                   
               
               
                 ttctaaggag caccacgaaa gcatcccaat tggtgggatg caggccgtgt ggagttctcg    120 
               
               
                   
               
               
                 tctgtagtgg acgagggctg ggtgcacaac aacaagcaag ccagacacac tattgggtcc    180 
               
               
                   
               
               
                 tgagacaaca ctcgggcgct agcacgaagt gttgtccctc catcttggtg gtggggtgtg    240 
               
               
                   
               
               
                 gtgtttgaga actggatagt ggttgcgagc atcaactgat cgcgtcgccg ttcgcggtgg    300 
               
               
                   
               
               
                 cgtgttcttt tgtgcaattt taaattcttt ggtttttgta gtgtttgtaa gtgtctaagg    360 
               
               
                   
               
               
                 gcgc                                                                 364 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 7 
               
               
                 &lt;211&gt; LENGTH: 402 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 7 
               
               
                   
               
               
                 aaggagcacc gattcgattc ccccgccgtc cgcgtagtcg tgggcagtag tgcggttggg     60 
               
               
                   
               
               
                 atatttcagc caggacctgt agtgggtgtc tggtgggtgc aaatgacaaa cgttgagccg    120 
               
               
                   
               
               
                 gcgcgggaaa gcgttggtga tggaactgct gaacacacta ttgggctttg agacaacagg    180 
               
               
                   
               
               
                 cccgtgcccc tttcgggggg tggcattccg ttgggagtgt cggcgtgttg ttgctccgct    240 
               
               
                   
               
               
                 ttggtggtgg ggtgtggtgt ttgatttgtg gatagtggtt gcgagcatct agcacgcagt    300 
               
               
                   
               
               
                 gtggctgggg gccttcgggt ttccggtctt gttgtgtgtt gatgtgcaat ttcttttgaa    360 
               
               
                   
               
               
                 actcattttt ggtttttgtg ttgtaagtgt ttaagggcgc at                       402 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 8 
               
               
                 &lt;211&gt; LENGTH: 459 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 8 
               
               
                   
               
               
                 aaggagcacc agttattttc ccccgccccc acgcctgtgg gatcagttgc ggttgggata     60 
               
               
                   
               
               
                 tttagtgcca gcacctgtag tgggtgtttc ggccggtgca cagcaaacgt tgatcgtctg    120 
               
               
                   
               
               
                 gtggggaaag ccgggcgtga aagattgcca gacacactat tgggctttga gacaacaagc    180 
               
               
                   
               
               
                 ccgtcgcctc tttgtcccga gtgtgggata tcggagaagg agcaccacga gacctgttgc    240 
               
               
                   
               
               
                 ccgcccacat cgtgtgggag ttcggtgact caggcgattc gggggttggt tgtggttgtc    300 
               
               
                   
               
               
                 gccctgcttt ggtggtgggg tgtggtgttt gatttgtgga tagtggttgc gagcatctga    360 
               
               
                   
               
               
                 acgcagagac ctgtgtgggt ttttgtgttc tgataatgca atttttattc ttccgagaat    420 
               
               
                   
               
               
                 attttttgat ctgttttgtg tgtaagtgtt taagggcgc                           459 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 9 
               
               
                 &lt;211&gt; LENGTH: 418 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 9 
               
               
                   
               
               
                 aaggagcacc acgagacctg ttgcccgccc acatcgtgtg ggagttcggt gactcaggcg     60 
               
               
                   
               
               
                 attcgttgga tggcctcaca cctgtagtgg gtgggggtct ggtgcacaac aaactttgag    120 
               
               
                   
               
               
                 aaactgccag acacactatt gggctttgag acaacaggcc ctgcggtgcc ggactcgttg    180 
               
               
                   
               
               
                 gagtcctggt tgccggccgc gagtcccgga agcgattctg gttcggacgg tgtctgttgt    240 
               
               
                   
               
               
                 tgctccatct ttggtggtgg ggtgtggtgt ttgatttgtg gatagtggtt gcgagcatct    300 
               
               
                   
               
               
                 agcacgcaag aggagtctgg gtttccttcg ggagcccggg tttttgttgt gtgtgtttga    360 
               
               
                   
               
               
                 tgtgcaattt ttttcttcta tttggtttta tctgtgttgt aagtgtttaa gggcgcat      418 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 10 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacteria 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 10 
               
               
                   
               
               
                 tggtggggtg tggtgtttga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 11 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacteria 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 11 
               
               
                   
               
               
                 tggatagtgg ttgcgagcat                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 12 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacteria 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 12 
               
               
                   
               
               
                 ccatcttggt ggtggggtgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 13 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacteria 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 13 
               
               
                   
               
               
                 cacactattg ggccctgagg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 14 
               
               
                 &lt;211&gt; LENGTH: 10 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacteria 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 14 
               
               
                   
               
               
                 aaggagcacc                                                            10 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 15 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting TB 
               
               
                       complex 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 15 
               
               
                   
               
               
                 cactcggact tgttccaggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 16 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting TB 
               
               
                       complex 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 16 
               
               
                   
               
               
                 tggtggggcg taggccgtga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 17 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting TB 
               
               
                       complex 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 17 
               
               
                   
               
               
                 ccccaactgg tggggcgtag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 18 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting TB 
               
               
                       complex 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 18 
               
               
                   
               
               
                 ccgtgagggg ttcttgtctg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 19 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting TB 
               
               
                       complex 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 19 
               
               
                   
               
               
                 gtagtgggcg agagccgggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 20 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting TB 
               
               
                       complex 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 20 
               
               
                   
               
               
                 catgacaaca aagttggcca                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 21 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting TB 
               
               
                       complex 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 21 
               
               
                   
               
               
                 caacactcgg acttgttcca                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 22 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting TB 
               
               
                       complex 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 22 
               
               
                   
               
               
                 gttccaggtg ttgtcccacc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 23 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting TB 
               
               
                       complex 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 23 
               
               
                   
               
               
                 cgagcatcaa tggatacgct                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 24 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium avium complex(MAC) 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 24 
               
               
                   
               
               
                 gttcatcgaa atgtgtaatt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 25 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium avium complex(MAC) 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 25 
               
               
                   
               
               
                 gtgtggagtc cctccatctt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 26 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium avium complex(MAC) 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 26 
               
               
                   
               
               
                 aaatgattgc cagacacact                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 27 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium avium complex(MAC) 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 27 
               
               
                   
               
               
                 ccctgagaca acactcggtc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 28 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 28 
               
               
                   
               
               
                 cgagccgtga ggaaccggtt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 29 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 29 
               
               
                   
               
               
                 ccgtgaggaa ccggttgcct                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 30 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 30 
               
               
                   
               
               
                 gtagtgggca cggtttggtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 31 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 31 
               
               
                   
               
               
                 ttggtgcaca acaaactttt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 32 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 32 
               
               
                   
               
               
                 caaacttttt tgactgccag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 33 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 33 
               
               
                   
               
               
                 acacactatt gggctttgag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 34 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 34 
               
               
                   
               
               
                 ctattgggct ttgagacaac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 35 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 35 
               
               
                   
               
               
                 aggcccgtgc cccttttggg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 36 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 36 
               
               
                   
               
               
                 tggcatccgg ttgcgggtgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 37 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 37 
               
               
                   
               
               
                 tagcacgcag aatcgtgtgg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 38 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium fortuitum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 38 
               
               
                   
               
               
                 ggttttgtgt gttgatgtgc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 39 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium chelonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 39 
               
               
                   
               
               
                 tttcccagcc gaatgagctt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 40 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium chelonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 40 
               
               
                   
               
               
                 ttgggaacat aaagcgagtt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 41 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium chelonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 41 
               
               
                   
               
               
                 gttactcgct tggtgaatat                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 42 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium chelonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 42 
               
               
                   
               
               
                 gttttataaa tcctgtccac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 43 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium chelonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 43 
               
               
                   
               
               
                 gtagtcggca aaacgtcgga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 44 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium chelonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 44 
               
               
                   
               
               
                 tcaatagaat tgaaacgctg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 45 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium chelonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 45 
               
               
                   
               
               
                 ggcaacacat tgtgttgtca                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 46 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium chelonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 46 
               
               
                   
               
               
                 taacaaacct cgctcgttta                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 47 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium abscessus 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 47 
               
               
                   
               
               
                 cataaagtag gcatctgtag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 48 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium abscessus 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 48 
               
               
                   
               
               
                 agtggatatc tacttggtga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 49 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium abscessus 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 49 
               
               
                   
               
               
                 taaacatagc ctcgctcgtt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 50 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium abscessus 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 50 
               
               
                   
               
               
                 cgttttcgag tggggctggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 51 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium abscessus 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 51 
               
               
                   
               
               
                 gagtggggct ggtttttgca                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 52 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium abscessus 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 52 
               
               
                   
               
               
                 ctcgttttcg agtggggctg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 53 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 53 
               
               
                   
               
               
                 tcaggcccgc ccacatcgtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 54 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 54 
               
               
                   
               
               
                 tgggggttcg gtgatctgat                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 55 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 55 
               
               
                   
               
               
                 cgattcgttg gatggccttt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 56 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 56 
               
               
                   
               
               
                 ggccgagccg gtagggaatg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 57 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 57 
               
               
                   
               
               
                 ccggcgaggg aaatcatcag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 58 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 58 
               
               
                   
               
               
                 acacactatt gggctttgag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 59 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 59 
               
               
                   
               
               
                 acaacaagcc cgtgcccctt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 60 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 60 
               
               
                   
               
               
                 ttttgggggt ggctctgcgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 61 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 61 
               
               
                   
               
               
                 tggcagggtc ggcgtgttgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 62 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 62 
               
               
                   
               
               
                 tgtttgattc gtggatagtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 63 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 63 
               
               
                   
               
               
                 gaatgcacag cgcttgtggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 64 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium vaccae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 64 
               
               
                   
               
               
                 gtgtgttcgg tgtaatgcaa                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 65 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium flavescens 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 65 
               
               
                   
               
               
                 tttattgttc ccccgtcccc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 66 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium flavescens 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 66 
               
               
                   
               
               
                 acgtgtgtgg gatcagtgcg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 67 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium flavescens 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 67 
               
               
                   
               
               
                 gttgggagat cagtgccggg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 68 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium flavescens 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 68 
               
               
                   
               
               
                 cctgtagtgg gtttccggtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 69 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium flavescens 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 69 
               
               
                   
               
               
                 cgtgagaagt ggtgtgggaa                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 70 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium flavescens 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 70 
               
               
                   
               
               
                 cactgctttg aggaatcatc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 71 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium flavescens 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 71 
               
               
                   
               
               
                 ggcccgttgt ttccctggcc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 72 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium flavescens 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 72 
               
               
                   
               
               
                 tgaacaggtg gctccctttt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 73 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium gordonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 73 
               
               
                   
               
               
                 cgacaacaag ctaagccaga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 74 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium gordonae 
               
               
                 &lt;400&gt; SEQUENCE: 74 
               
               
                   
               
               
                 tgttcttttt gtgcaatttt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 75 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium gordonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 75 
               
               
                   
               
               
                 gcatcaaaat gtatgcgttg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 76 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium gordonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 76 
               
               
                   
               
               
                 aaaatgtatg cgttgtcgtt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 77 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium gordonae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 77 
               
               
                   
               
               
                 cggcaacgtg ttctttttgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 78 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 78 
               
               
                   
               
               
                 ttgagttgtg gatagtggtt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 79 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 79 
               
               
                   
               
               
                 gtcgagtgtt tagagagtaa                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 80 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 80 
               
               
                   
               
               
                 cgcactgggc gcattccgag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 81 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 81 
               
               
                   
               
               
                 gtgggggctg ggtgcacaac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 82 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 82 
               
               
                   
               
               
                 caacgttgaa aacaagatcg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 83 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 83 
               
               
                   
               
               
                 aattacgaac aacaacaagc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 84 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 84 
               
               
                   
               
               
                 ttgcgagatc atcaactgcc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 85 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 85 
               
               
                   
               
               
                 aggagtcctt gggggtttct                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 86 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 86 
               
               
                   
               
               
                 ggtggccggc ttttgtgctg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 87 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 87 
               
               
                   
               
               
                 ggcacactgt tgggtcctga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 88 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 88 
               
               
                   
               
               
                 ggcaacaggc ccgtttgtgc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 89 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 89 
               
               
                   
               
               
                 ccccgggtgg gggtgggtgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 90 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 90 
               
               
                   
               
               
                 gtgttgttgt cgcctcacac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 91 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 91 
               
               
                   
               
               
                 taacaagcag atttttggtc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 92 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 92 
               
               
                   
               
               
                 tggtctgttt gttttgcaat                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 93 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 93 
               
               
                   
               
               
                 ttttgtttct tggtttttgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 94 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 94 
               
               
                   
               
               
                 gtttgtaagt gtttaagggc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 95 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 95 
               
               
                   
               
               
                 gcatggtgga tgccttggca                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 96 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 96 
               
               
                   
               
               
                 ctttttcccc cgtgcctcac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 97 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 97 
               
               
                   
               
               
                 atgggtgagg gtttttgcgg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 98 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 98 
               
               
                   
               
               
                 ttgggacagt gtttgccggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 99 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 99 
               
               
                   
               
               
                 gcctgtagtg ggtggccggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 100 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium terrae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 100 
               
               
                   
               
               
                 ggtggccggt ggtgcagagg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 101 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium scrofluceum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 101 
               
               
                   
               
               
                 cctgaggcaa cactcggctc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 102 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium scrofluceum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 102 
               
               
                   
               
               
                 tcggctcgtt ctgagtggtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 103 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium scrofluceum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 103 
               
               
                   
               
               
                 taaacggatg cgtggccgaa                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 104 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium scrofluceum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 104 
               
               
                   
               
               
                 caacagcaaa tgattgccag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 105 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium scrofluceum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 105 
               
               
                   
               
               
                 ccctccatct tggtggtggg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 106 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium scrofluceum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 106 
               
               
                   
               
               
                 tgcgagcatc taaacggatg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 107 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium scrofluceum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 107 
               
               
                   
               
               
                 gtggccgaac ggtggcgtgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 108 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium scrofluceum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 108 
               
               
                   
               
               
                 tcgttgaaat gtgtaatttc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 109 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium ka 
               
               
                 &lt;400&gt; SEQUENCE: 109 
               
               
                   
               
               
                 gtggggtgca agccgtgagg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 110 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium kansasii 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 110 
               
               
                   
               
               
                 gcgtgttctt ttgtgcaatt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 111 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium kansasii 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 111 
               
               
                   
               
               
                 gcaactgtaa atgaatcacc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 112 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium kansasii 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 112 
               
               
                   
               
               
                 ctggatgcgc tgccgttcgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 113 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                        Mycobacterium szulgai 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 113 
               
               
                   
               
               
                 aacactcagg cttggccaga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 114 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium szulgai 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 114 
               
               
                   
               
               
                 caattggatg cgctgccctc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 115 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium szulgai 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 115 
               
               
                   
               
               
                 tcgtggtggc gtgttctttt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 116 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium szulgai 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 116 
               
               
                   
               
               
                 gtgcaatttt aattctttgg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 117 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium marinum 
               
               
                       and Mycobacterium ulcerans 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 117 
               
               
                   
               
               
                 aacaacaagc aagccagaca                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 118 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium marinum and Mycobacterium ulcerans 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 118 
               
               
                   
               
               
                 atctctgttg gtttcgggat                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 119 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium marinum and Mycobacterium ulcerans 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 119 
               
               
                   
               
               
                 ccttttggtg gcgtgttctg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 120 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium gastri 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 120 
               
               
                   
               
               
                 aacagcaagc aagccagaca                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 121 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium gastri 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 121 
               
               
                   
               
               
                 ctcgtccaag agtgttgtcc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 122 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium gastri 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 122 
               
               
                   
               
               
                 gcttgtcttg gactcgtcca                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 123 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium gastri 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 123 
               
               
                   
               
               
                 cagggtagcg tgttcttttg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 124 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium xenopi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 124 
               
               
                   
               
               
                 catctggcaa agactgtggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 125 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium xenopi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 125 
               
               
                   
               
               
                 agggagcacc gtaaacgcat                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 126 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium xenopi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 126 
               
               
                   
               
               
                 cccgcgtggg gtggggtgtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 127 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium xenopi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 127 
               
               
                   
               
               
                 ggttcggcgt gttgtggcgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 128 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium xenopi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 128 
               
               
                   
               
               
                 cgggccgagg tgttgggcag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 129 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium xenopi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 129 
               
               
                   
               
               
                 caggcagtaa ccgccggcac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 130 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium xenopi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 130 
               
               
                   
               
               
                 caggcagtaa ccgccggcac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 131 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium xenopi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 131 
               
               
                   
               
               
                 tccgcgtggt ggcggggtgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 132 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium xenopi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 132 
               
               
                   
               
               
                 agactgtggt aagcggtttt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 133 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium xenopi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 133 
               
               
                   
               
               
                 tgttgagtgt tttctggtgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 134 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium genavense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 134 
               
               
                   
               
               
                 cattgaatag tggttgcgag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 135 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium genavense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 135 
               
               
                   
               
               
                 aacaacaggc aatcgccgga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 136 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium genavense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 136 
               
               
                   
               
               
                 tcggccgact gaggtcgacg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 137 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium genavense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 137 
               
               
                   
               
               
                 gacgtggtgt ccctccatct                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 138 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium genavense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 138 
               
               
                   
               
               
                 ttgagcattg aatagtggtt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 139 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium genavense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 139 
               
               
                   
               
               
                 gcgagcatct agacggatgc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 140 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium genavense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 140 
               
               
                   
               
               
                 gttccccagt ggtgcgcgtt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 141 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium genavense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 141 
               
               
                   
               
               
                 cgtcaaaaat gtgtaatttt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 142 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium malmoense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 142 
               
               
                   
               
               
                 agcatctaaa cggatgcgct                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 143 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium malmoense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 143 
               
               
                   
               
               
                 tggtggggtg caagccgtga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 144 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium malmoense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 144 
               
               
                   
               
               
                 ccagtccgcg tggtgtcccc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 145 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium malmoense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 145 
               
               
                   
               
               
                 tgcccgtaga cgcgtattcg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 146 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium malmoense 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 146 
               
               
                   
               
               
                 tttgtgtaat ttcttctttg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 147 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium simiae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 147 
               
               
                   
               
               
                 acaacaacag gcaatcgcca                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 148 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium simiae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 148 
               
               
                   
               
               
                 actcggccga cttcggttga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 149 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium simiae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 149 
               
               
                   
               
               
                 gagcatctaa atgaacgcgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 150 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium simiae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 150 
               
               
                   
               
               
                 tccaattggt ggggtgtgag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 151 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium simiae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 151 
               
               
                   
               
               
                 tgcacaacaa caggcaatcg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 152 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium simiae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 152 
               
               
                   
               
               
                 cgacttcggt tgaagtggtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 153 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium simiae 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 153 
               
               
                   
               
               
                 tacgtgttcg ttttgtgtaa                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 154 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 154 
               
               
                   
               
               
                 gacactctcc gttggggagg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 155 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 155 
               
               
                   
               
               
                 gtgtgagccg tgaggagctg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 156 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 156 
               
               
                   
               
               
                 gagcgctgta gtggcgccgg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 157 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 157 
               
               
                   
               
               
                 cttggtgcac agcaaacgtt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 158 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 158 
               
               
                   
               
               
                 gagatgcggt gtgggaaacg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 159 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 159 
               
               
                   
               
               
                 ctgtttcgat ggactgccag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 160 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 160 
               
               
                   
               
               
                 acacactatt gggccctgag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 161 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 161 
               
               
                   
               
               
                 acaacaggcc cgcgttcccg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 162 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 162 
               
               
                   
               
               
                 tcccgttggg ggcggggggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 163 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 163 
               
               
                   
               
               
                 gggtgtgttg ttgccctgct                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 164 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 164 
               
               
                   
               
               
                 gctgtagtgg cgccggcttg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 165 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium smegmatis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 165 
               
               
                   
               
               
                 aaacgctgtt tcgatggact                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 166 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium shimoidei 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 166 
               
               
                   
               
               
                 aacaacaagc gagaagccga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 167 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium shimoidei 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 167 
               
               
                   
               
               
                 ggcttccgca gtgggcggaa                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 168 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium shimoidei 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 168 
               
               
                   
               
               
                 gggcgcgggc tgggtgcaca                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 169 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium shimoidei 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 169 
               
               
                   
               
               
                 gcgagaagcc gagcacactg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 170 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium shimoidei 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 170 
               
               
                   
               
               
                 cccgggccct ttggggttgg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 171 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium shimoidei 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 171 
               
               
                   
               
               
                 aagcaaaact tggttgtttt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 172 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium shimoidei 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 172 
               
               
                   
               
               
                 gtttgtcgag ttgttttctt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 173 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium habana 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 173 
               
               
                   
               
               
                 aacaacaggc aatcgccaga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 174 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium habana 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 174 
               
               
                   
               
               
                 aacactccaa ttgggtgggg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 175 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium habana 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 175 
               
               
                   
               
               
                 ggttctcgtc tgtagtggac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 176 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium habana 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 176 
               
               
                   
               
               
                 tgcacaacaa caggcaatcg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 177 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium habana 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 177 
               
               
                   
               
               
                 ggccgacttc ggttgaagtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 178 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium habana 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 178 
               
               
                   
               
               
                 ggggtgtggt gtttgagtat                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 179 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium habana 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 179 
               
               
                   
               
               
                 gagcatctaa atgaacgcgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 180 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium habana 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 180 
               
               
                   
               
               
                 tacgtgttcg ttttgtgtaa                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 181 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium farcinogen 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 181 
               
               
                   
               
               
                 gttggtgatg gactgccaga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 182 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium farcinogen 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 182 
               
               
                   
               
               
                 cgcgggaaag cgttggtgat                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 183 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium farcinogen 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 183 
               
               
                   
               
               
                 cgatgtcgtg ggcagtagtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 184 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium farcinogen 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 184 
               
               
                   
               
               
                 ggatatttca gccagcatct                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 185 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium farcinogen 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 185 
               
               
                   
               
               
                 tgtctggtgg gtgcaaatga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 186 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium farcinogen 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 186 
               
               
                   
               
               
                 cggcgcggga aagcgttggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 187 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium farcinogen 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 187 
               
               
                   
               
               
                 gggaaagcgt tggtgatgga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 188 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium farcinogen 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 188 
               
               
                   
               
               
                 tctagcacgc agagtgtggc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 189 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium farcinogen 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 189 
               
               
                   
               
               
                 tgggggcctt cgggtttctt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 190 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium asiaticum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 190 
               
               
                   
               
               
                 catcccaatt ggtgggatgc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 191 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium asiaticum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 191 
               
               
                   
               
               
                 cacaacaaca agcaagccag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 192 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium asiaticum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 192 
               
               
                   
               
               
                 cactcgggcg ctagcacgaa                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 193 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium asiaticum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 193 
               
               
                   
               
               
                 aactgatcgc gtcgccgttc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 194 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 194 
               
               
                   
               
               
                 cagtagtgcg gttgggatat                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 195 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 195 
               
               
                   
               
               
                 ttcgattccc ccgccgtccg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 196 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 196 
               
               
                   
               
               
                 cagccaggac ctgtagtggg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 197 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 197 
               
               
                   
               
               
                 tggtgggtgc aaatgacaaa                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 198 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 198 
               
               
                   
               
               
                 gagccggcgc gggaaagcgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 199 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 199 
               
               
                   
               
               
                 gtgatggaac tgctgaacac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 200 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 200 
               
               
                   
               
               
                 ggctttgaga caacaggccc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 201 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 201 
               
               
                   
               
               
                 cggggggtgg cattccgttg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 202 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 202 
               
               
                   
               
               
                 cggcgtgttg ttgctccgct                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 203 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 203 
               
               
                   
               
               
                 tagtggttgc gagcatctag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 204 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 204 
               
               
                   
               
               
                 agtgtggctg ggggccttcg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 205 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium porcinum 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 205 
               
               
                   
               
               
                 ggtcttgttg tgtgttgatg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 206 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 206 
               
               
                   
               
               
                 tgccagcacc tgtagtgggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 207 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 207 
               
               
                   
               
               
                 gatcgtctgg tggggaaagc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 208 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 208 
               
               
                   
               
               
                 cgggcgtgaa agattgccag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 209 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 209 
               
               
                   
               
               
                 agcccgtcgc ctctttgtcc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 210 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 210 
               
               
                   
               
               
                 cgagtgtggg atatcggaga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 211 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 211 
               
               
                   
               
               
                 ggagcaccac gagacctgtt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 212 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 212 
               
               
                   
               
               
                 cgcccacatc gtgtgggagt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 213 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 213 
               
               
                   
               
               
                 tcggtgactc aggcgattcg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 214 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 214 
               
               
                   
               
               
                 gggtgtggtg tttgatttgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 215 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium acapulcensis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 215 
               
               
                   
               
               
                 cgagcatctg aacgcagaga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 216 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 216 
               
               
                   
               
               
                 acctgttgcc cgcccacatc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 217 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 217 
               
               
                   
               
               
                 gtgtgggagt tcggtgactc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 218 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 218 
               
               
                   
               
               
                 ttcgttggat ggcctcacac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 219 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 219 
               
               
                   
               
               
                 tgcacaacaa actttgagaa                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 220 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 220 
               
               
                   
               
               
                 acaggccctg cggtgccgga                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 221 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 221 
               
               
                   
               
               
                 tcgttggagt cctggttgcc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 222 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 222 
               
               
                   
               
               
                 ggccgcgagt cccggaagcg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 223 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 223 
               
               
                   
               
               
                 gtctgttgtt gctccatctt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 224 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 224 
               
               
                   
               
               
                 ggtgtggtgt ttgatttgtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 225 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 225 
               
               
                   
               
               
                 atagtggttg cgagcatcta                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 226 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 226 
               
               
                   
               
               
                 acgcaagagg agtctgggtt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 227 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium diernhoferi 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 227 
               
               
                   
               
               
                 ttgttgtgtg tgtttgatgt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 228 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 228 
               
               
                   
               
               
                 gagcaccacg aaaagcaccc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 229 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 229 
               
               
                   
               
               
                 ctggtggggt gcgagccgtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 230 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 230 
               
               
                   
               
               
                 gcgagccgtg aggggttccc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 231 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 231 
               
               
                   
               
               
                 aggggttccc gtctgtagtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 232 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 232 
               
               
                   
               
               
                 acgggggccg ggtgcgcaac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 233 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 233 
               
               
                   
               
               
                 caaatgattg ccagacacac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 234 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 234 
               
               
                   
               
               
                 tattgggcct gagacaacac                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 235 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 235 
               
               
                   
               
               
                 cggtccgtcc gtgtggagtc                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 236 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 236 
               
               
                   
               
               
                 tccatcttgg tggtggggtg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 237 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 237 
               
               
                   
               
               
                 ggtgtttgag tattggatag                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 238 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 238 
               
               
                   
               
               
                 tggttgcgag catctagatg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 239 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 239 
               
               
                   
               
               
                 gcgcatggtc tccgtggccg                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 240 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacterium paratuberculosis 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 240 
               
               
                   
               
               
                 gttcatcgaa atgtgtaatt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 241 
               
               
                 &lt;211&gt; LENGTH: 20 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting 
               
               
                       Mycobacteria sp. 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 241 
               
               
                   
               
               
                 aaaagctgtt gtttgacggt                                                 20 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 242 
               
               
                 &lt;211&gt; LENGTH: 25 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of primer for amplifying Mycobacteria 
               
               
                       ITS selected from the sequence of 16S rRNA in Mycobacteria 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 242 
               
               
                   
               
               
                 tggatccgac gaagtcgtaa caagg                                           25 
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 243 
               
               
                 &lt;211&gt; LENGTH: 25 
               
               
                 &lt;212&gt; TYPE: DNA 
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence 
               
               
                 &lt;220&gt; FEATURE: 
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of primer for amplifying Mycobacteria 
               
               
                       ITS selected from the sequence of 23S rRNA in Mycobacteria 
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 243 
               
               
                   
               
               
                 tggatcctgc caaggcatcc accat                                           25