Patent Publication Number: US-2021163979-A1

Title: Polynucleotides and polypeptides of plant and bacterial origin for protecting plants from pathogenic fungi

Description:
FIELD OF THE INVENTION 
     The present invention relates to polynucleotides and polypeptides of plant and bacterial origin which are involved in enhancing resistance of plants towards pathogenic fungi and/or Oomycetes, particularly to fungi inducing root rot and stalk rot in plants, and use thereof for controlling plant diseases associated with the fungal pathogens and for producing genetically engineered plants having increased resistance to the pathogenic fungi. 
     BACKGROUND OF THE INVENTION 
     During all developmental stages, plants are exposed to an extremely wide range of biotic and abiotic stress conditions leading to plant diseases. In the production of crop plants, damages caused by biotic stresses, particularly by pathogenic agents, which may be further enhanced under conditions of abiotic stress, pose a major problem and significantly affect the crop yield and profitability. 
     Many plant diseases are caused by plant pathogenic fungi, and damages to both monocotyledonous and dicotyledonous crop plants are of billions of US$ loss in yield in the U.S. only. For example, stalk rot, caused by a complex of  Fusarium  spp. and other fungi is one of the most serious challenges in maize production. At present, the majority of both inbreed and hybrid maize lines are susceptible.  Fusarium graminearum  (Fg) and  Fusarium verticillioides  (Fv) are the two main causal agents of stalk rot caused by  Fusarium  spp. in maize, but more than 10 additional  Fusarium  spp. can cause stalk rot. Natural infection is initiated by a mixture of the local  Fusarium  spp., but typically during the progress of the disease one species predominates.  Gibberella  stalk rot (caused by  Fusarium graminearum  Schwabe) is more prevalent in maize grown in cool regions; while  Fusarium  stalk rot (caused by  Fusarium verticillioides ) is most common in dry, warm regions.  Fusarium graminearum  and other  Fusarium  species are also responsible for  Fusarium  head blight (FHB) of wheat, which is a major disease problem for wheat and barley production worldwide, and for various root rots in wide range of different hosts.  Colletotrichum  spp. infects many grain crops such as barley, wheat, sorghum and corn.  C. graminicola  is one of the other major pathogens causing mainly maize stalk rots and being capable of infecting many other parts of the corn plant. It has been suggested that  C. graminicola  behaves as a wilt fungus which efficiently colonizes and moves through the fiber cells that surround the vascular bundles and underlie the epidermal cells in the stalk rind. Movement through the mostly non-living fibers may allow the fungus to avoid host defenses, providing a base from which it can invade adjacent parenchyma cells. 
     Powdery mildews are fungal diseases caused by different species of fungi in the order Erysiphales that affect a wide range of plants including cereals, grasses, vegetables, ornamentals, weeds, shrubs, fruit trees, broad-leaved shade and forest trees. 
     Traditionally, plant diseases have been controlled by agronomic practices that include crop rotation, the use of agrochemicals, and conventional breeding techniques. The use of chemicals to control plant pathogens, while being effective, increases the production costs, and moreover, is opposed to by the public and government regulators due to the increased awareness to the harmful effects of such chemicals on the ecosystem and animal health. 
     Upon the plant recognition of an agent as a pathogen, an array of biochemical responses is activated by the plant. As of today, it is acknowledged that the initial plant response involves induction of several local responses in the cells immediately surrounding the infection site. In the hypersensitive response, cells contacted by the pathogen, and often neighboring cells, rapidly collapse and dry in a necrotic fleck. Other responses include the deposition of callose, the physical thickening of cell walls by lignification, and the synthesis of various antibiotic small molecules and proteins. Genetic factors in both the host and the pathogen determine the specificity of these local responses, which can be very effective in limiting spread of the infection. 
     Resistance to  Fusarium , as an example, is a polygenic trait and can be seen as consisting of two major components: (1) resistance to initial penetration, and (2) resistance to the spreading of the pathogen in host tissue. Though there is no evidence of complete resistance to  Fusarium  stalk rot in maize, genetic variation for resistance exists within maize germplasm. Resistance to  C. graminicola  is also primarily quantitative, although a few sources of major gene resistance have been described. 
     The constantly growing volume of research regarding the mechanisms involved in plant resistance to pathogens and the genetic basis of such mechanism, together with advances in biotechnology have presented new opportunities for protecting plants against pathogen infection through genetic engineering. 
     Many genes have been identified to participate in the plant defense mechanisms. For example, Sanghyun S et al. (2008. J Exp Bot. 2008:59(9):2371-8) showed that transgenic wheat expressing a barley class II chitinase exhibited enhanced resistance against  F. graminearum  in greenhouse and field conditions. Zhu X et al. (2012. Funct Integr Genomics. 12(3):481-488) described that overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to  Cochliobolus sativus  and  Fusarium graminearum  in transgenic wheat. Perochon A et al. (2015. Plant Physiol 169(4):2895-2906) reported the functional characterization of an orphan gene ( Triticum aestivum  Fusarium Resistance Orphan Gene [TaFROG]) as a component of resistance to  Fusarium  head blight (FHB). Zuo D Y et al. (2016. Phytopatol. 106(6):614-623) showed that a deoxynivalenol-activated methionyl-tRNA synthetase gene from wheat encodes a nuclear localized protein and protects plants against  Fusarium  pathogens infection and mycotoxins. Dowd P F and Johnson E T (2016. J Plant Res. 129(1):13-20) showed that the maize peroxidase Px5, the pericarp expression of which has been shown to be associated with resistance to  Aspergillus flavus  growth and to insects in a set of inbred plant lines has a highly conserved sequence which enhances fungal and insect resistance. 
     Many defense response genes are induced in wheat and other plants during  F. graminearum  infection and may play a role in reducing FHB. These response genes were thus investigated in an attempt to produce resistant wheat lines. Mackintosh C A et al. (2007. Plant Cell Rep 26(4):479-488) examined overexpression of the defense response genes alpha-1-purothionin, thaumatin-like protein 1 (tlp-1), and beta-1,3-glucanase in wheat, and reported that all the genes reduced at least part of the disease symptoms. A beta-1,3-glucanase transgenic line had enhanced resistance, showing lower FHB severity, deoxynivalenol (DON) mycotoxin concentration, and percent of visually scabby kernels (VSK) compared to a control plant. Sasaki K et al. (2016. J Biotechnol 228:3-7) also showed that overexpression of TAD1 ( Triticum aestivum  defensin 1), a protein induced during cold acclimation in winter wheat and encoding a plant defensin with antimicrobial activity, increased resistance against  Fusarium graminearum  in the transformed wheat plants. 
     Various additional genetic manipulation of gene expression for improving resistance to pathogenic fungi has also been reported. For example, Brewer H C et al. (2014. BMC Plant Biol 14(1):317) showed that mutations in the  Arabidopsis  homoserine kinase gene DMR1 confer enhanced resistance to  F. culmorum  and  F. graminearum . Tundo S et al. (2016. Mol Plant Microbe Interact 29(8):629-639) produced transgenic plants with a combination of genes encoding proteins involved in inhibiting the activity of cell wall-degrading enzymes (CWDEs) secreted by pathogens to breach the plant cell-wall barrier. They showed that pyramiding polygalacturonase (PG) inhibiting protein (PGIP2) and TAXI-III, a xylanase inhibitor that controls the activity of xylanases, enhanced resistance against  Fusarium graminearum , while pyramiding PGIP2 and pectin methyl esterase inhibitor (PMEI) did not reach the same effect. Li X et al. (2015. Mol Plant Microbe Interact 28(11):1237-1246) demonstrated that transgenic wheat expressing a barley UDP-glucosyltransferase detoxifies deoxynivalenol and provides high levels of resistance to  Fusarium graminearum.    
     Among others, International Application Publication Nos. WO/2006/085965 and WO 2006/091219 disclose methods for protecting plants from plant pathogenic fungi by enhancing fungal pathogen resistance in a plant using the nucleotide sequences disclosed therein. Further disclosed therein are methods comprising introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antifungal polypeptide as well as transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antifungal polypeptide or variant or fragment thereof. 
     U.S. Pat. No. 9,359,615 discloses plants which overexpress a p33 kD or BURP protein, or an ortholog thereof, and exhibit an increased pre-formed resistance to pathogens, particularly fungal pathogens. 
     U.S. Pat. No. 9,732,354 discloses new gene that is able to provide plants with resistance against pathogens, particularly  Verticillium, Ralstonia  or  Fusarium . The gene is typical for Brassicaceae, but may confer resistance to other plants. Further provided are host cells with a nucleotide construct encoding the protein and methods for enhancing the pathogen resistance of plants. 
     International (PCT) Patent Application Publication No. WO 2018/131037 to the Applicant of the present invention discloses polynucleotides and polypeptides associated with increased resistance of plant to pathogenic fungi, particularly to fungi inducing root rot and stalk rot in plants, and use thereof for controlling plant fungal pathogens and for producing transgenic plants having increased resistance to pathogenic fungi. 
     Intact microorganisms, particularly bacteria, which can survive on or be toxic to fungi, have been used as biocontrol agents. For example, U.S. Pat. No. 9,485,994 discloses methods and compositions for control of pathogenic fungal or Oomycetous infection. Particularly, the patent discloses an antifungal or an anti-Oomycetous composition comprising bacteria of the genus  Collimonas  and bacteria of the genus  Bacillus , together exhibiting a synergistic antifungal or a synergistic anti-Oomycetous effect, and methods of use thereof. However, it is difficult both to apply biological organisms to large areas and to cause such living organisms to remain in the treated area or on the treated plant species for an extended time, and therefore use of intact bacteria as biocontrol agents is still limited. 
     Therefore, attempts were made to identify bacterial proteins and genes encoding same responsible for the biocontrol activity. Targets, mechanism of action and prospective applications of anti-fungal proteins of various organisms, including bacteria are reviewed, for example, by Theis and Stahl (Theis T and Stahl U. 2004 CMLS, Cell. Mol. Life Sci. 61:437-455). Proteomic approach taken to investigate  Bacillus  strains having anti-fungal activity revealed the presence of lytic enzymes, cellulases, proteases, 1,4-β-glucanases and hydrolases, all of which contribute to degradation of the pathogen cell wall. Further proteomic investigations showed that proteins involved in metabolism, protein folding, protein degradation, translation, recognition and signal transduction cascade play an important role in the control of  Fusarium oxysporum  (Baysal O. et al., 2013. Plose One 8(1):e53182). Li et al., (Li Jing et al., J Zhejiang Univ Sci B 10(4):264-272) describe the purification of a particular antifungal protein from  Bacillus subtilis , designated B29I, which exhibited inhibitory activity on mycelial growth of  Fusarium oxysporum, Rhizoctonia solani, Fusarium moniliforme , and  Sclerotinia sclerotiorum . Yadav et al. (Yadav V et al., 2010. Intl J Microbiol, Article ID 196363, doi:10.1155/2010/196363) described the isolation of an antifungal protein from  Escherichia coli  BL21 (PPEBL21), predicted to be alcohol dehydrogenase (ADH). Additional example of enzymes having a significant role in the anti-fungal activity of bacteria includes chitinases (see, e.g. Hjort K et al., 2013. Appl Microbiol Biotechnol DOI 10.1007/s00253-013-5287-x). 
     Song et al. (Song C et al., 2015. BMC Genomics 16:1103) explored the genomic traits of the fungus-feeding bacterial genus  Collimonas , and Haack et al. (Haack F S et al., 2016. Frontiers in Microbiology 7 Article 1668) investigated the molecular keys of the interactions of  Janthinobacterium  and  Duganella  species with the plant pathogenic fungi  Fusarium graminearum.    
     However, as of today, there is a need for further developed methods and compositions for protecting plants from fungal pathogen. 
     SUMMARY OF THE INVENTION 
     The present invention provides isolated polynucleotides, constructs comprising same and isolated polypeptides useful in conferring and/or enhancing resistance of a plant towards pathogenic fungi and/or Oomycetes. The present invention further provides genetically altered plants and plant cells with enhanced resistance to pathogenic fungi and/or Oomycetes and methods for producing and selecting same as well as compositions comprising the polypeptides of the invention useful in protecting plants from the pathogenic fungi and/or Oomycetes. 
     According to one aspect, the present invention provides a method for enhancing the resistance of a plant or a part thereof to at least one pathogenic fungus and/or Oomycete, comprising modulating the expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506 within at least one cell of the plant or part thereof, thereby enhancing the resistance of said plant or part thereof to the at least one pathogenic fungus and/or Oomycete compared to the resistance of a control plant. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the method comprises modulating the expression and/or activity of at least one polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the at least one polypeptide is encoded by a polynucleotide comprising a nucleic acid sequence at least 80% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, 81-301. Each possibility represents a separate embodiment of the present invention. 
     According to other embodiments, the at least one polypeptide is encoded by a polynucleotide comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-305. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, enhancing the resistance of the plant or part thereof to the pathogenic fungus and/or Oomycete comprises enhancing the expression and/or activity of at least one polypeptide compared to its expression and/or activity in the control plant. 
     According to certain exemplary embodiments, the polypeptide the expression and/or activity of which is to be enhanced comprises an amino acid sequence at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-506. Each possibility represents a separate embodiment of the present invention. According to some embodiments, the polypeptide the expression and/or activity of which is to be enhanced comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-508. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the polypeptide the expression and/or activity of which is to be enhanced is an endogenous polypeptide of the at least one cell. According to other embodiments, the polypeptide the expression and/or activity of which is to be enhanced is a polypeptide heterologous to the at least one plant cell. According to certain embodiments, enhancing the expression and/or activity of the polypeptide comprises modulating the expression of an endogenous polynucleotide encoding said polypeptide within the at least one cell of the plant or part thereof. 
     Modulating, according to certain embodiments enhancing, the expression of the endogenous polynucleotide can be affected at the genomic and/or the transcript level using a variety of methods that induce the transcription and/or translation of the polypeptide. 
     According to certain embodiments, enhancing the expression and/or activity of the endogenous polypeptide comprises subjecting the at least one cell of the plant or part thereof to genome editing using artificially engineered nucleases as is known in the art. 
     According to certain embodiments, isolated nucleic acids which serve as promoter or enhancer elements may be introduced in an appropriate position as to enhance transcription of the endogenous polynucleotide. According to some embodiments, the regulatory element is selected from the group consisting of, but not limited to, a promoter and an enhancer. 
     According to certain embodiments, the polypeptide the expression of which is to be enhanced is encoded by a polynucleotide at least 80% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41, 43-61, 63-79, 81, 83-301. According to some embodiments, the polypeptide the expression of which is to be enhanced is encoded by a polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41, 43-61, 63-79, 81, 83-305. 
     According to other embodiments, at least one mutation may be inserted within the endogenous polynucleotide as long as the mutation results in enhanced expression of the encoded polypeptide. Any method for mutagenesis as is known in the art can be used according to the teachings of the present invention including chemical mutagenesis, radio-mutagenesis and site directed mutagenesis, for example using genome editing techniques. 
     According to certain embodiments, enhancing the resistance of the plant to the pathogenic fungus and/or Oomycete comprises reducing the expression of at least one polypeptide compared to its expression and/or activity in the control plant. According to certain exemplary embodiments, the polypeptide the expression and/or activity of which is to be reduced comprises an amino acid sequence at least 80% identical to the amino acid sequence set forth in any one of SEQ ID NO:328, SEQ ID NO:361, and SEQ ID NO:348. According to further exemplary embodiments, the polypeptide the expression and/or activity of which is to be reduced comprises the amino acid sequence set forth in any one of SEQ ID NO:328, SEQ ID NO:361 and SEQ ID NO:348. 
     According to certain embodiments, enhancing the resistance of the plant to the pathogenic fungus and/or Oomycete comprises reducing the expression and/or activity of at least one polynucleotide compared to its expression in the control plant. According to certain exemplary embodiments, the polynucleotide having reduced expression comprises a nucleic acid sequence at least 80% identical to the nucleic acid sequence set forth in any one of SEQ ID NO:42, SEQ ID NO:82, and SEQ ID NO:62. According to certain exemplary embodiments, the polynucleotide having reduced expression comprises the nucleic acid sequence set forth in any one of SEQ ID NO:42, SEQ ID NO:82 and SEQ ID NO:62. 
     Any method as is known in the art for reducing the expression and/or activity of a plant endogenous protein and the polynucleotide encoding same can be used according to the teachings of the resent invention. 
     According to certain embodiments, reducing the expression and/or activity of the polypeptide comprises down-regulating the expression of the endogenous polynucleotide encoding said polypeptide within the at least one cell of the plant or part thereof. 
     According to certain embodiments, reducing the expression and/or activity of the polypeptide comprises modulating the endogenous polynucleotide as to encode a non-functional polypeptide. 
     According to certain embodiments, expression of the polynucleotide is affected at the genomic and/or the transcript level using a variety of molecules that interfere with transcription and/or translation (e.g., antisense, siRNA, Ribozyme, or DNAzyme) of the polynucleotide. Inserting a mutation into the polynucleotide, including deletions, insertions, site specific mutations, mutations mediated by artificially engineered nucleases (including zinc-finger nucleases (ZFNs), transcription-activator like effector nucleases (TALENs) and CRISPR/Cas system) can be also used, as long as the mutations result in down-regulation of the gene expression or in the production of non-functional protein. Alternatively, expression can be inhibited at the protein level using, e.g., antagonists, enzymes that cleave the polypeptide, and the like. 
     According to additional aspect, the present invention provides a method for enhancing the resistance of a plant or a part thereof to at least one pathogenic fungus and/or Oomycete, comprising enhancing the expression and/or activity of at least one polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, an active fragment and/or a variant thereof within at least one cell of the plant or part thereof, thereby enhancing the resistance of said plant or part thereof to the at least one pathogenic fungus and/or Oomycete compared to the resistance of a control plant. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the method comprises enhancing the expression and/or activity of at least one polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, an active fragment and/or a variant thereof. Each possibility represents a separate embodiment of the present invention. 
     According to the certain exemplary embodiments, the fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:4661, 4647-4660, and 4662-4799. 
     According to certain embodiments, the at least one polypeptide is encoded by a polynucleotide comprising a nucleic acid sequence at least 70% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443, a fragment and/or a variant thereof. Each possibility represents a separate embodiment of the present invention. 
     According to other embodiments, the at least one polypeptide is encoded by a polynucleotide comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:783, 592-782, 784-2443, a fragment and/or a variant thereof. Each possibility represents a separate embodiment of the present invention. 
     Enhancing the polypeptide expression can be affected at the genomic and/or the transcript and/or translation level using a variety of methods that induce the transcription and/or translation of the polypeptide. 
     According to certain embodiments, enhancing the expression and/or activity of the polypeptide comprises expressing an exogenous polynucleotide encoding said at least one polypeptide within the at least one cell of the plant or the part thereof. 
     According to certain embodiments, enhancing the expression and/or activity of the polypeptide comprises transforming at least one cell of the plant or part thereof with an exogenous polynucleotide encoding the polypeptide, thereby producing a transgenic plant over-expressing said polypeptide. 
     Any method as is known in the art for introducing an exogenous polynucleotide into a plant cell can be used according to the teachings of the present invention. According to some embodiments, the exogenous polynucleotide is transformed into the plant cell using a suitable vector. 
     According to certain embodiments, genome editing is employed to edit the genome of the at least one cell as to express a heterologous polypeptide of the invention. 
     According to certain exemplary embodiments, the polynucleotide the expression of which is to be enhanced comprises a nucleic acid sequence at least 80% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41, 43-61, 63-79, 81, and 83-301. Each possibility represents a separate embodiment of the present invention. According to some embodiments, the polynucleotide the expression of which is to be enhanced comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41, 43-61, 63-79, 81, and 83-305. 
     According to further exemplary embodiments, the polynucleotide the expression of which is to be enhanced comprises a nucleic acid sequence at least 70% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443 and a fragment thereof. According to some embodiments, the polynucleotide the expression of which is to be enhanced comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443 and a fragment thereof. 
     Gene stacking is often desirable in the field of plant genetic engineering, leading to a desired phenotype and/or improvement of a desired phenotype. According to certain aspects, the present invention provides a method for enhancing the resistance of a plant or a part thereof to at least one pathogenic fungus and/or Oomycete, comprising transforming at least one cell of the plant or part thereof with at least one DNA construct comprising nucleic acid sequence encoding at least two polypeptides, wherein each polypeptide is capable of enhancing resistance to the at least one pathogenic fungus and/or Oomycete, thereby enhancing the resistance of said plant or part thereof to said at least one pathogenic fungus and/or Oomycete compared to the resistance of a control plant. According to certain embodiments, the present invention provides a method for enhancing the resistance of a plant or a part thereof to at least one pathogenic fungus and/or Oomycete, comprising transforming at least one cell of a plant or a part thereof with a DNA construct comprising a first polynucleotide encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:311 and a second polynucleotide encoding a polypeptide designated LFS57 having the amino acid sequence set forth in SEQ ID NO:617 of International Application Publication No. WO 2018/131037, thereby enhancing the resistance of said plant or part thereof to the at least one pathogenic fungus and/or Oomycete compared to the resistance of a control plant. 
     According to certain embodiments, the first polynucleotide comprises the nucleic acid sequence set forth in SEQ ID NO:66 and the second polynucleotide comprises the nucleic acid sequence set forth in any one of SEQ ID NO:101 and SEQ ID NO:165 of WO 2018/131037. 
     According to certain embodiments, the present invention provides a method for enhancing the resistance of a plant or a part thereof to at least one pathogenic fungus and/or Oomycete, comprising transforming at least one cell of a plant or a part thereof with a DNA construct comprising a first polynucleotide encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:310 and a second polynucleotide encoding a polypeptide designated LFS46 having the amino acid sequence set forth in SEQ ID NO:608 of International Application publication No. WO 2018/131037, thereby enhancing the resistance of said plant or part thereof to the at least one pathogenic fungus and/or Oomycete compared to the resistance of a control plant. 
     According to certain embodiments, the first polynucleotide comprises the nucleic acid sequence set forth in SEQ ID NO:65 and the second polynucleotide comprises the nucleic acid sequence set forth in any one of SEQ ID NO:92 and SEQ ID NO:157 of WO 2018/131037. 
     According to some embodiments, the control plant is a plant not manipulated to have modulated expression and/or activity of the polypeptide. According to some embodiments, the control plant is of the same species. According to some embodiments, the control plant comprises the same genetic background. 
     According to another aspect, the present invention provides a method for producing a population of plants each having an enhanced resistance to at least one pathogenic fungus and/or Oomycete, comprising the steps of:
         (a) modulating the expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, and 361-506 within at least one cell of each plant of a plant population as to produce a genetically engineered plant population;   (b) inoculating each plant of the genetically engineered plant population with the at least one pathogenic fungus and/or Oomycete; and   (c) selecting plants showing an enhanced resistance to said at least one pathogenic fungus and/or Oomycete compared to a control plant or to a pre-determined resistance score value;       

     thereby producing a population of genetically engineered plants having enhanced resistance to said at least one pathogenic fungus and/or Oomycete. 
     According to certain embodiments, the method comprises modulating the expression and/or activity of at least one polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508. According to certain embodiments, the method comprises modulating the expression and/or activity of at least one polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs:507-508. According to certain embodiments, the method comprises modulating the expression and/or activity of at least one polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs:507-509. According to other embodiments, the method comprises modulating the expression and/or activity of at least one polypeptide having the amino acid sequence set forth in SEQ ID NO:509. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the method comprises enhancing the expression and/or activity of the at least one polypeptide. According to these embodiments, the method comprises enhancing the expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-506. According to some embodiments, the method comprises enhancing the expression and/or activity of at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-509. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the method comprises reducing the expression and/or activity of the at least one polypeptide. According to these embodiments, the method comprises reducing the expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:328, SEQ ID NO:361 and SEQ ID NO:348. According to some embodiments, the method comprises reducing the expression and/or activity of at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:328, SEQ ID NO:361 and SEQ ID NO:348. 
     According to yet another aspect, the present invention provides a method for producing a population of plants each having an enhanced resistance to at least one pathogenic fungus and/or Oomycete, comprising the steps of:
         (a) enhancing the expression and/or activity of at least one polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, an active fragment and/or variant thereof within at least one cell of each plant of a plant population as to produce a genetically engineered plant population;   (b) inoculating each plant of the genetically engineered plant population with the at least one pathogenic fungus and/or Oomycete; and   (c) selecting plants showing an enhanced resistance to said at least one pathogenic fungus and/or Oomycete compared to a control plant or to a pre-determined resistance score value;       

     thereby producing a population of genetically engineered plants having enhanced resistance to said at least one pathogenic fungus and/or Oomycete. 
     According to certain embodiments, the method comprises modulating the expression and/or activity of at least one polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs:2635, 2444-2634, 2636-4252, an active fragment and/or a variant thereof. 
     According to the certain exemplary embodiments, the polypeptide fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:4661, 4647-4660, and 4662-4799. The methods by which the expression and/or activity of the at least polypeptide can be enhanced or reduced are as is known in the art and as described hereinabove. 
     According to certain embodiments, the method comprises modulating the expression of at least one polynucleotide comprising a nucleic acid sequence at least 80% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, 81-301. According to some embodiments, the method comprises modulating the expression of at least one polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, and 81-305. According to some embodiments, the method comprises modulating the expression of at least one polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:304-305. According to some embodiments, the method comprises modulating the expression of at least one polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:304-306. According to some embodiments, the method comprises modulating the expression of at least one polynucleotide comprising a nucleic acid sequence set forth in SEQ ID NO:306. Each possibility represents a separate embodiment of the present invention. According to some embodiments, modulating the expression of the at least one polynucleotide comprises enhancing its expression. According to these embodiments, the polynucleotide is at least 80% identical to a polynucleotide having the nucleic acid sequence selected from the group consisting of 40, 80, 22-39, 41, 43-61, 63-79, 81, and 83-301. According to some embodiments, the polynucleotide comprises the nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41, 43-61, 63-79, 81, and 83-306. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, enhancing the expression comprises transforming the at least one cell of the plant or part thereof with a polynucleotide at least 80% identical to a polynucleotide having an amino acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, 81, and 83-301. According to certain embodiments, enhancing the expression comprises transforming the at least one cell of the plant or part thereof with a polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, 81, and 83-306. 
     According to some embodiments, modulating the expression of the at least one polynucleotide comprises reducing its expression. According to these embodiments, the polynucleotide is at least 80% identical to a polynucleotide having the nucleic acid sequence selected from the group consisting of SEQ ID NO:42, SEQ ID NO:82 and SEQ ID NO:62. According to some embodiments, the polynucleotide comprises the nucleic acid sequence selected from the group consisting of SEQ ID NO:42, SEQ ID NO:82 and SEQ ID NO:62. 
     According to certain embodiments, the method comprises enhancing the expression of at least one polynucleotide comprising a nucleic acid sequence at least 70% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443, a variant and/or a fragment thereof. According to some embodiments, the method comprises enhancing the expression of at least one polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443, a variant and/or a fragment thereof. 
     The methods by which the expression of the at least one polynucleotide can be enhanced or reduced are as is known in the art and as described hereinabove. 
     According to additional aspect, the present invention provides a method for selecting a plant having an enhanced resistance to at least one pathogenic fungus and/or Oomycete, comprising the steps of:
         (a) providing a plurality of plants each comprising at least one cell with modulated expression and/or activity of a polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506.   (b) inoculating the plurality of plants with the at least one pathogenic fungus and/or Oomycete; and   (c) selecting plants showing an enhanced resistance to said at least one pathogenic fungus and/or Oomycete compared to a control plant or to a pre-determined resistance score value.       

     According to certain embodiments, the method comprises providing a plurality of plants each having a modulated expression and/or activity of at least one polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508. Each possibility represents a separate embodiment of the present invention. According to other embodiments, the method comprises providing a plurality of plants each having a modulated expression and/or activity of a polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs:507-509. Each possibility represents a separate embodiment of the present invention. According to some embodiments, the method comprises providing a plurality of plants each having a modulated expression and/or activity of a polypeptide having the amino acid sequence set forth in SEQ ID NO:509. 
     According to certain embodiments, the method comprises providing a plurality of plants each having modulated expression of at least one polynucleotide comprising a nucleic acid sequence at least 80% identical to a polynucleotide having the nucleic acid sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-301. According to some embodiments, the at least one polynucleotide comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, 81-305. Each possibility represents a separate embodiment of the present invention. According to some embodiments, the at least one polynucleotide comprises a nucleic acid sequence set forth in SEQ ID NO:306. 
     According to certain embodiments, the modulated expression and/or activity is selected from enhanced expression and/or activity and reduced expression and/or activity. Modulating the expression can be performed by any method as is known in the art and as described hereinabove. The polypeptides and polynucleotides the expression of which is to be enhanced or reduced are as described hereinabove. According to yet further aspect, the present invention provides a method for selecting a plant having an enhanced resistance to at least one pathogenic fungus and/or Oomycete, comprising the steps of:
         (a) providing a plurality of plants each comprising at least one cell with enhanced expression and/or activity of a polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, an active fragment and/or a variant thereof;   (b) inoculating the plurality of plants with the at least one pathogenic fungus and/or Oomycete; and   (c) selecting plants showing an enhanced resistance to said at least one pathogenic fungus and/or Oomycete compared to a control plant or to a pre-determined resistance score value.       

     According to certain embodiments, the method comprises providing a plurality of plants each having an enhanced expression and/or activity of at least one polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs:2635, 2444-2634, 2636-4252, an active fragment and/or a variant thereof. Each possibility represents a separate embodiment of the present invention. 
     According to the certain exemplary embodiments, the fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:4661, 4647-4660, and 4662-4799. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the method comprises providing a plurality of plants each having enhanced expression of at least one polynucleotide comprising a nucleic acid sequence at least 70% identical to a polynucleotide having the nucleic acid sequence set forth in any one of SEQ ID NOs:783, 592-782, 784-2443. According to some embodiments, the at least one polynucleotide comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:4783, 592-782, 784-2443. 
     According to certain embodiments, the control plant is a plant not manipulated to have modulated expression and/or activity of the polypeptide. According to some embodiments, the control plant is of the same species. According to some embodiments, the control plant comprises the same genetic background. 
     According to certain embodiments, the pre-determined resistance score value is obtained by a method comprising the steps of inoculating a plurality of corresponding plants susceptible to the at least one pathogenic fungus or Oomycete; scoring the infection degree; and setting an average resistance score value. 
     Modulating (enhancing or reducing) the expression and/or activity of the polypeptide can be achieved as described hereinabove and by any other method as is known in the art. 
     According to certain embodiments, the plant part is selected from the group consisting of seeds, roots, shoots, leaves, flowers and the like. Each possibility represents separate embodiment of the present invention. According to certain exemplary embodiments, the plant part is a root. Tissue cultures comprising cells derived from the plant having a modulated expression and/or activity of a polypeptide of the invention are also encompassed within the scope of the present invention. 
     According to additional aspect, the present invention provides a genetically engineered plant having enhanced resistance to at least one fungus and/or Oomycete compared to a non-engineered control plant, the genetically engineered plant comprising at least one cell having modified expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506 compared to the polypeptide expression and/or activity in the non-engineered control plant. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having enhanced expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-506. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having reduced expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:328, 361 and 348. 
     According to additional aspect, the present invention provides a genetically engineered plant having enhanced resistance to at least one fungus and/or Oomycete compared to a non-engineered control plant, the genetically engineered plant comprising at least one cell having enhanced expression and/or activity of at least one polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, an active fragment or a variant thereof compared to the polypeptide expression and/or activity in the non-engineered control plant. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having modified expression and/or activity of at least one polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the genetically engineered plant comprises at least one cell having modified expression and/or activity of at least one polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NOs:507-508. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having enhanced expression and/or activity of at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-508. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having reduced expression and/or activity of at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:328, 361, and 348. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having enhanced expression and/or activity of at least one polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, an active fragment and/or a variant thereof. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having modified expression of at least one polynucleotide comprising a nucleic acid sequence at least 80% identical to a polynucleotide having the nucleic acid sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-301. According to some embodiments, the at least one polynucleotide comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, 81-305. According to some embodiments, the at least one polynucleotide comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:302-305. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having enhanced expression of at least one polynucleotide comprising a nucleic acid sequence at least 80% identical to a polynucleotide having the nucleic acid sequence set forth in any one of SEQ ID NOs: 40, 80, 22-39, 41, 43-61, 63-79, 81, and 83-301. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having reduced expression of at least one polynucleotide comprising a nucleic acid sequence at least 80% identical to a polynucleotide having the nucleic acid sequence set forth in any one of SEQ ID NOs:42, 82 and 62. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having enhanced expression of at least one polynucleotide comprising a nucleic acid sequence at least 70% identical to a polynucleotide having the nucleic acid sequence set forth in any one of SEQ ID NOs:783, 592-782, 784-2443. According to some embodiments, the at least one polynucleotide comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises a polynucleotide encoding a modified form of the at least one polypeptide, wherein the modified form has reduced or no activity compared to the unmodified form, thereby having an enhanced resistance to the at least one fungus. 
     According to additional aspect, the present invention provides an isolated or recombinant polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, and 362-506, wherein the polypeptide, when expressed in a plant, is capable of modulating the resistance of the plant to at least one pathogenic fungus and/or Oomycete. According to certain embodiments, the polynucleotide encodes a polypeptide comprising the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 362-506. According to certain embodiments, the polynucleotide encodes a polypeptide comprising the amino acid sequence set forth in any one of SEQ ID NOs:507-508. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the polypeptide, when expressed in a plant, is capable of enhancing the resistance of the plant to at least one pathogenic fungus and/or Oomycete, said polypeptide is at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, and 362-506. According to certain embodiments, said polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, and 362-508. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the polynucleotide encoding a polypeptide capable of modulating the resistance of the plant to at least one pathogenic fungus and/or Oomycete when expressed in said plant comprises a nucleic acid sequence at least 80% identical to a nucleic acids sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-301. According to other embodiments, the polynucleotide comprises the nucleic acid sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-301. According to yet further embodiments, the polynucleotide comprises the nucleic acid sequence set forth in any one of SEQ ID NOs:302-305. 
     According to certain embodiments, the polynucleotide encoding a polypeptide capable of enhancing the resistance of the plant to at least one pathogenic fungus and/or Oomycete when expressed in said plant comprises a nucleic acid sequence at least 80% identical to a nucleic acids sequence set forth in any one of SEQ ID NOs: 40, 80, 22-39, 41, 43-61, 63-79, 81, and 83-301. According to other embodiments, the polynucleotide comprises the nucleic acid sequence set forth in any one of SEQ ID NOs: 40, 80, 22-39, 41, 43-61, 63-79, 81, and 83-301. According to yet further embodiments, the polynucleotide comprises the nucleic acid sequence set forth in any one of SEQ ID NOs:302-305. 
     According to yet additional aspect, the present invention provides an isolated or recombinant polynucleotide encoding a polypeptide comprising an amino acid sequence at least 70% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, wherein the polypeptide, when expressed in a plant, is capable of enhancing the resistance of the plant to at least one pathogenic fungus and/or Oomycete. According to certain embodiments, the polynucleotide encodes a polypeptide comprising the amino acid sequence set forth in any one of SEQ ID NOs:2635, 2444-2634, 2636-4252. 
     According to yet another aspect, the present invention provides an isolated or recombinant polynucleotide, a fragment or a variant thereof, the polynucleotide comprising a nucleic acid sequence at least 80% identical to a nucleic acids sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41, 43-61, 63-79, 81, and 83-301, wherein said polynucleotide, when expressed in a plant, is capable of enhancing the resistance of the plant to at least one pathogenic fungus and/or Oomycete. According to certain embodiments, the polynucleotide comprises the nucleic acid sequence set forth in any one if SEQ ID NOs:40, 80, 22-39, 41, 43-61, 63-79, 81, and 83-305. 
     According to yet further aspect, the present invention provides an isolated or recombinant polynucleotide, a fragment or a variant thereof, the polynucleotide comprising a nucleic acid sequence at least 70% identical to a nucleic acids sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443, wherein said polynucleotide, when expressed in a plant, is capable of enhancing the resistance of the plant to at least one pathogenic fungus and/or Oomycete. According to certain embodiments, the polynucleotide comprises the nucleic acid sequence set forth in any one if SEQ ID NOs:783, 592-782, 784-2443. 
     According to additional aspect, the present invention provides a recombinant polynucleotide optimized for polypeptide expression in a bacterial expression system, wherein the polypeptide is capable of inhibiting the growth of at least one pathogenic fungus and/or Oomycete. 
     According to certain exemplary embodiment the bacterium is  Escherichia coli . According to these embodiments, the polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 4468, 4450-4467, and 4469-4646. According to certain embodiments, the recombinant polynucleotide comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:4271, 4253-4270, and 4272-4449. 
     According to certain embodiments, the recombinant polynucleotide optimized for polypeptide expression in the bacterial expression system further comprises said bacterium signal peptide. 
     According to a further aspect, the present invention provides a fungicidal composition comprising an effective amount of at least one polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof, wherein the fungicidal composition is effective in inhibiting the growth and/or development of at least one plant pathogenic fungi and/or Oomycetes. According to certain embodiments, the fungicidal composition comprises at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 4468, 4661, 2444-2634, 2636-4252, 4450-4467, 4469-4660, and 4662-4799. According to some embodiments, the fungicidal composition comprises at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:4468, 4661, 4450-4467, 4469-4660, and 4662-4799. According to certain embodiments, the fungicidal composition further comprises at least one agriculturally compatible agent selected from the group consisting of a carrier, a stabilizer, a diluent, a surfactant, a mineral and an adjuvant. 
     According to yet additional aspect, the present invention provides a fungicidal composition comprising bacteria comprising at least one polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof, wherein the fungicidal composition is effective in inhibiting the growth and/or development of at least one plant pathogenic fungi and/or Oomycetes. According to certain embodiments, the bacteria comprise at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 4468, 4661, 2444-2634, 2636-4252, 4450-4467, 4469-4660, and 4662-4799. According to some embodiments, the bacteria comprise at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:4468, 4661, 4450-4467, 4469-4660, and 4662-4799. Each possibility represents a separate embodiment of the present invention. The bacteria can be of the same species or of different species, with the proviso that the bacteria have no adverse effect on a plant. The bacteria can be alive or dead. According to certain exemplary embodiments, the bacteria are selected from the group consisting of  Bacillus subtilis Lactococcus lacti, Corynebacterium glutamicum ; and  Bacillus brevis.    
     According to certain embodiments, the fungicidal composition further comprises at least one agriculturally compatible agent selected from the group consisting of a carrier, a stabilizer, a diluent, a surfactant, a mineral and an adjuvant. 
     According to additional aspect, the present invention provides a nucleic acid construct comprising a polynucleotide according to some embodiments of the present invention, further comprising at least one regulatory element for directing the expression of the polynucleotide within a plant cell. According to certain embodiment, the regulatory element is a promoter. The promoter can be endogenous or heterologous to the plant comprising the nucleic acid construct. 
     The polypeptides and polynucleotides disclosed herein may be used to confer resistance to a wide variety of fungal and Oomycetous pathogens that cause commercial damage to crop and ornamental plants. 
     According to certain embodiments, the fungal or Oomycetous pathogens can be one or more fungi or Oomycetes from a class selected from the group consisting of Ascomycetes, Plasmodiophoromycetes, Chytridiomycetes, Zygomycetes, Basidiomycetes, Deuteromycetes, and Sordariomycetes. Each possibility represents a separate embodiment of the present invention. 
     According to some embodiments, the fungal pathogens can be one or more fungi from a genus selected from the group consisting of  Fusarium, Colletotrichum, Geotrichum, Aspergillus, Alternaria, Athelia, Botryosphaeria, Botrytis, Cryphonectria, Choanephora, Cercospora, Magnaporthe Monilinia, Mycosphaerella, Melampsora, Puccinia, Phakopsora, Rhizoctonia, Septoria, Uromyces, Ustilago  and  Verticillium.    
     According to some embodiments, the Oomycetous pathogen can be from the class Oomycetes (synonym Peronosporomycetes). In some embodiments, said Oomycetous infection comprises infection by an Oomycete from a genus selected from the group consisting of  Blumeria, Macrophomina, Oidium, Pythium , and  Phytophthora . Each possibility represents a separate embodiment of the present invention. According to some embodiments, the fungal or Oomycetous pathogen is selected from the group consisting of  Botrytis cinerea, Mycosphaerella graminicola, Mycosphaerella fijiensis, Septoria lycopersici, Magnaporthe oryza, Rhizoctonia solani, Ustilago maydis, Sclerotium rolfsii , and  Blumeria  graminis. 
     According to certain exemplary embodiments, the fungus of the genus  Fusarium  is selected from the group consisting of  Fusarium verticilloides  and  Fusarium graminearum . According to additional exemplary embodiments, the  F. verticilloides  is  F. verticillioides  strain A-00149-FGSC 7600. According to further exemplary embodiments, the  F. graminearum  is  F. graminearum  strain CBS 110260. 
     According to other exemplary embodiments, the fungus of the genus  Colletotrichum  is  Colletotrichum graminicola.    
     The polynucleotides and polypeptides of the present invention can be used to confer resistance to any plant type. According to certain embodiments, the plant is a cereal plant. According to some embodiments, the cereal plant is selected from the group consisting of maize, wheat, barley, sorghum, rice, oat, and rye. Each possibility represents a separate embodiment of the present invention. According to certain exemplary embodiments, the cereal plant is maize ( Zea mays ). According to other embodiments, the plant is a field-crop plant. According to some embodiments, the field crop plant is selected from the group consisting of tomato, potato, sweet potato, cassava, beets, ginger, horseradish, radish, ginseng, turnip, any root or tuber crop, pepper, eggplant, ground cherry, tomatillo, okra, other fruiting vegetables, cucumber, cantaloupe, melon, muskmelon, squash, watermelon and other cucurbit plants. 
     Further embodiments and the full scope of applicability of the present invention will become apparent from the detailed description given hereinafter. However, it should be understood that the detailed description and specific examples, while indicating preferred embodiments of the invention, are given by way of illustration only, since various changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description. 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIG. 1  is a schematic illustration of the pQ6sVN plasmid. pQ6sVN used for expression of the isolated polynucleotide sequences of some embodiments of the invention in  Brachypodium. “ 35S(V)”=35S promoter (SEQ ID NO:37); “NOS ter”=nopaline synthase terminator; “Bar_GA”=BAR open reading frame optimized for expression in  Brachypodium  (SEQ ID NO:39); “Hyg”=Hygromycin resistance gene. “Ubi1 promoter”=SEQ ID NO:11; the isolated polynucleotide sequences of some embodiments of the invention were cloned into the Multiple cloning site of the vector (downstream of the “35S(V)” promoter) using one or more of the indicated restriction enzyme sites. 
         FIGS. 2A-H  depict an exemplary design of Homology Directed Repair according to some embodiments of the invention.  FIG. 2A  depicts the sequence of the endogenous 5′-upstream flanking region of the genomic sequence GRMZM2G069095 (SEQ ID NO:45).  FIG. 2B  depicts the sequence of the endogenous 3′-downstream flanking region of the genomic sequence GRMZM2G069095 having the nucleic acid sequence set forth in SEQ ID NO:46.  FIG. 2C  depicts the sequence of the 5′-UTR gRNA (SEQ ID NO:43).  FIG. 2D  depicts the sequence of the 5′-UTR gRNA without NGG nucleotides (SEQ ID NO:47).  FIG. 2E  depicts the sequence of the 3′-UTR gRNA (SEQ ID NO:44).  FIG. 2F  depicts the sequence of the 3′-UTR gRNA after cut (SEQ ID NO:48).  FIG. 2G  depicts the coding sequence (from the “ATG” start codon to the “TGA” termination codon, marked by bold and underlined) of the desired LFS127_H4 sequence (SEQ ID NO:50) encoding the polypeptide set forth by SEQ ID NO:487.  FIG. 2H  depicts the exemplary repair template (SEQ ID NO:49) which includes (1) the upstream flanking region (1 kbp) sequence including part of the gRNA after cutting (SEQ ID NO:47; shown in bold and italics); (2) 5′ UTR of genomic DNA from Cas9 cutting site to ATG; (3) the coding sequence (CDS) of the desired LFS127_H4 sequence (SEQ ID NO:50) marked in lower case with the start (ATG) and the stop (TGA) codons marked in bold and underlined; (4) 3′ UTR of genomic DNA from the stop codon to Cas9 cutting site including the predicted part of the gRNA after cutting (SEQ ID NO:48, shown in bold and underlined and (5) the downstream flanking region (1 kbp) sequence. 
         FIG. 3A-E  depicts an exemplary design of polynucleotide knockout (KO) using CRISPR/CAS system.  FIG. 3A  depicts the sequence of the KO gRNA (SEQ ID NO:51);  FIG. 3B  depicts the sequence of the KO gRNA after cut (SEQ ID NO:52);  FIG. 3C  depicts the coding sequence (from the “ATG” start codon to the “TAG” termination codon, marked by bold and underlined) of the desired LFS132 sequence (SEQ ID NO:53);  FIG. 3D  (targeted region in bold) and  FIG. 3E  depict the anticipated change in the coding sequence of the exemplified KO gene (SEQ ID NO:54). 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The present invention discloses means and methods for conferring and/or enhancing the resistance of a plant to pathogenic fungi and/or Oomycetes. Particularly, the present invention provides isolated polypeptides conferring or enhancing plant resistance to pathogenic fungi and/or Oomycetes, isolated polynucleotides encoding same, nucleic acid constructs comprising the polynucleotides and plant cells transformed with same. The present invention further provides methods for producing and selecting plants having increased resistance to at least one pathogenic fungus and/or Oomycete and plant with enhanced resistance to the at least one pathogenic fungus and/or Oomycete, in which the expression of the polynucleotides and/or polypeptides of the invention is modulated. In additional aspects, the present invention provides fungicidal compositions comprising isolated fungicidal polypeptides of the invention and/or bacteria comprising same. 
     The present invention is based in part on bioinformatics tools that have been used to identify polynucleotides associated with resistance or reduced sensitivity of plants to at least one pathogenic fungus or pathogenic Oomycete, as well as to identify polynucleotides of bacterial origin that encode for proteins with potential antifungal activity. Cereal plants, including maize ( Zea ), wheat ( Triticum ), millet ( Sitaria italica ) and the closely related  Aegilops  and  Brachypodium  species were used as representative genera to identify genes overexpressed or downregulated in plants showing increased resistance to fungal/Oomycetes infection. Genes comprising the nucleic acids sequence set forth in any one of SEQ ID NOs:22-62 and 302-303, encoding polypeptides having the amino acid sequence set forth in any one of SEQ ID NOs:308-348, 507, and 510 were identified. Homologous genes and encoded proteins were also identified in wider genera of plant, as described in details and presented in Table 7 hereinbelow. Bacteria isolated from soils known to contain  Fusaria  species were used for screening and identification of potential anti-fungal bacterial proteins. Genes comprising the nucleic acids sequence set forth in any one of SEQ ID NOs:592-789, encoding polypeptides having the amino acid sequence set forth in any one of SEQ ID NOs:2444-2641 were identified. Homologous genes and encoded proteins were also identified in wider genera of bacteria, as described in details and presented in Table 16 hereinbelow. 
     Polynucleotides of plant origin according to some embodiments of the present invention were cloned into binary vectors, and transformed into plants of the species  Brachypodium distachyon  (Example 6 hereinbelow) to validate the effect of the genes on the resistance of the transformed plants towards the fungi/Oomycetes (Example 7). 
     Polynucleotides of bacterial origin according to some embodiments of the present invention were cloned, transformed into  E. coli , and the expressed proteins were isolated (Examples 14-15). The anti-fungal effect of the isolated proteins was validated by their ability to inhibit the growth of  Fusarium verticillioides  mycelia (Example 16, Table 21). 
     Definitions 
     The terms “comprise”, “comprising”, “includes”, “including”, “having” and their conjugates mean “including but not limited to”. 
     The term “consisting of” means “including and limited to”. 
     The term “consisting essentially of” means that the composition, method or structure may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure. 
     As used herein, the singular form “a”, “an” and “the” include plural references unless the context clearly dictates otherwise. For example, the term “a compound” or “at least one compound” may include a plurality of compounds, including mixtures thereof. 
     Throughout this application, various embodiments of this invention may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible sub ranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed sub ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range. 
     Whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range. The phrases “ranging/ranges between” a first indicate number and a second indicate number and “ranging/ranges from” a first indicate number “to” a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween. 
     As used herein the term “method” refers to manners, means, techniques and procedures for accomplishing a given task including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the agricultural, chemical, pharmacological, biological, biochemical and medical arts. 
     When reference is made to particular sequence listings, such reference is to be understood to also encompass sequences that substantially correspond to its complementary sequence as including minor sequence variations, resulting from, e.g., sequencing errors, cloning errors, or other alterations resulting in base substitution, base deletion or base addition, provided that the frequency of such variations is less than 1 in 50 nucleotides, alternatively, less than 1 in 100 nucleotides, alternatively, less than 1 in 200 nucleotides, alternatively, less than 1 in 500 nucleotides, alternatively, less than 1 in 1000 nucleotides, alternatively, less than 1 in 5,000 nucleotides, alternatively, less than 1 in 10,000 nucleotides. 
     As used herein, the phrase “exogenous polynucleotide” refers to a heterologous nucleic acid sequence which is not naturally expressed within the plant (e.g., a nucleic acid sequence from a different species) or to an endogenous nucleic acid of which overexpression in the plant is desired. The exogenous polynucleotide may be introduced into the plant in a stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule and/or a polypeptide molecule. The term “endogenous” as used herein refers to a polynucleotide or polypeptide which is naturally present and/or naturally expressed within a plant or a cell thereof. 
     The terms “modulating”, “modifying” and “altering” with reference to the expression or activity of a polynucleotide, gene, polypeptide or a protein within a cell or a plurality of cells, particularly plant cell(s), are used herein interchangeably and refer to changing their level of expression within the cell, particularly plant cell. The change can be an increase or a decrease; and it can be measured as compared to any one of the polynucleotide, gene, polypeptide and/or protein level within the same cell(s) before modulation and as compared to the level in a control plant or an average level from a plurality of control plants in which the expression was not modified by man. 
     According to certain embodiments, the control plant is a wild type plant not manipulated to have modulated expression and/or activity of the polynucleotide and/or polypeptide. According to some embodiments, the control plant is of the same species. According to some embodiments, the control plant comprises the same genetic background. 
     According to certain embodiments, the examined plant and the control plant are grown under the same growing conditions. 
     As used herein, the term “resistance” with regard to plants pathogenic fungus and/or Oomycete refers to a plant that is resistant to infection by a fungal or Oomycetous pathogen or resistant to the symptoms of fungal or Oomycetous pathogen infection. For example, a plant resistant to a fungal or Oomycetous pathogen can exhibit a lack of infection, or reduced symptoms of infection, when challenged with a pathogen. As another example, a plant resistant to a fungal or Oomycetous pathogen can be infected by the fungal or Oomycetous pathogen and yet exhibit a reduced number or degree of symptoms of said infection. As yet another example, a plant resistant to a fungal or Oomycetous pathogen can be infected by the pathogen and exhibit one or more symptoms of infection by the pathogen and yet exhibit a reduction in an effect of the infection or symptom thereof. For instance, a plant resistant to a fungal or Oomycetous pathogen can be infected by the pathogen, and exhibit one or more symptoms selected from the group consisting of leaf wilt, leaf or vascular discoloration (e.g., yellowing), spike bleaching etc., and yet not exhibit a reduction in yield loss in comparison to a plant that is not resistant (susceptible) to the fungal or Oomycetous pathogen. 
     Accordingly, “confer resistance to a pathogenic fungus and/or Oomycete” or “enhanced resistance to a pathogenic fungus and/or Oomycete” refer to a phenotype in which a plant has greater health, growth, multiplication, fertility, vigor, strength (e.g., stem strength and resistance), yield, or less severe symptoms associated with infection of the pathogenic fungus or Oomycete during or after a fungal or Oomycete infection than an organism that does not have enhanced resistance to the pathogen. Where a plant is tested for resistance, a control plant is used to assess the degree of the plant resistance. According to certain embodiments of the present invention, the control plant is a plant not manipulated to have modified expression of at least one polynucleotide and/or polypeptide of the present invention. The control plant is typically, but not necessarily of the same species as the examined plant. According to some embodiments the control plant is of the same specifies and has the same genetic background as the examined plant. The enhancement can be manifested as an increase of 0.1%, 0.2%, 0.3%, 0.5%, 0.75%, 1%, 1.5%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 15%, 17%, 20%, 25%, 30%, 35%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, or more in health, growth, multiplication, fertility, vigor, strength, or yield, as compared to a control plant. The enhancement can be a decrease of 0.1%, 0.2%, 0.3%, 0.5%, 0.75%, 1%, 1.5%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 15%, 17%, 20%, 25%, 30%, 35%, 40%, 50%, 60%, 70%, 80%, 90%, or 100% in the symptoms associated with the pathogenic fungus and/or Oomycete as compared to the control plant. According to certain exemplary embodiments, the examined plant and the control plant are grown under the same conditions. 
     According to certain embodiments of the invention, enhancing the resistance of a plant to a pathogenic fungus comprises enhancing the expression and/or activity of a polypeptide of the invention within at least one cell of the plant. As used herein, the expression of a polynucleotide or polypeptide of the invention is “enhanced” or “up-regulated” if the level of the polynucleotide or polypeptide is enhanced by at least 50%, i.e. the polynucleotide or polypeptide level is at least 1.5-fold higher compared to its level in a control plant or compared to a predetermined threshold level. According to some embodiments, the level of the polynucleotide or polypeptide expression is enhanced by at least 60%, 70%, 80%, 90%, 100%, 200%, 300% and more. 
     According to certain embodiments, the pre-determined resistance score value is obtained by inoculating a population of corresponding plants susceptible to the at least one pathogenic fungus, scoring the infection degree and setting an average resistance score value. 
     According to certain embodiments of the invention, enhancing the resistance of a plant to a pathogenic fungus comprises reducing the expression and/or activity of a polynucleotide and/or polypeptide of the invention within at least one cell of the plant. As used herein, the expression of a polynucleotide or polypeptide of the invention is “reduced”, “inhibited”, “down regulated” or “knocked out” or “knocked down” if the level of the polynucleotide or polypeptide is reduced by at least 30% compared to its level in a control plant or compared to a predetermined threshold level. According to certain embodiments, the level of the polynucleotide or polypeptide is reduced by at least 40%, 50%, 60%, 70%, 80%, 90% and more. According to some embodiments, the term “reduced expression” refers to 100% inhibition or “knockout” of a polynucleotide function and/or expression. 
     As used herein the term “polynucleotide” refers to a single or double stranded nucleic acid sequence which is isolated and provided in the form of an RNA sequence, a complementary polynucleotide sequence (cDNA), a genomic polynucleotide sequence and/or a composite polynucleotide sequences (e.g., a combination of the above). 
     As used herein the phrase “complementary polynucleotide sequence” refers to a sequence, which results from reverse transcription of messenger RNA using a reverse transcriptase or any other RNA dependent DNA polymerase. Such a sequence can be subsequently amplified in vivo or in vitro using a DNA dependent DNA polymerase. 
     The term “isolated” refers to at least partially separated from the natural environment e.g., from a plant cell. 
     As used herein the phrase “genomic polynucleotide sequence” refers to a sequence derived (isolated) from a chromosome and thus it represents a contiguous portion of a chromosome. 
     As used herein the phrase “composite polynucleotide sequence” refers to a sequence, which is at least partially complementary and at least partially genomic. A composite sequence can include some exonal sequences required to encode the polypeptide of the present invention, as well as some intronic sequences interposing therebetween. The intronic sequences can be of any source, including of other genes, and typically will include conserved splicing signal sequences. Such intronic sequences may further include cis acting expression regulatory elements. 
     It should be noted that the nucleic acid sequence of a polynucleotide encoding a polypeptide which is provided in the sequence listing as a single strand refers to the sense direction which is equivalent to the mRNA transcribed from the polynucleotide. 
     When reference is made to particular sequence listings, such reference is to be understood to also encompass sequences that substantially correspond to its complementary sequence as including minor sequence variations, resulting from, e.g., sequencing errors, cloning errors, or other alterations resulting in base substitution, base deletion or base addition, provided that the frequency of such variations is less than 1 in 50 nucleotides, alternatively, less than 1 in 100 nucleotides, alternatively, less than 1 in 200 nucleotides, alternatively, less than 1 in 500 nucleotides, alternatively, less than 1 in 1000 nucleotides, alternatively, less than 1 in 5,000 nucleotides, alternatively, less than 1 in 10,000 nucleotides. 
     As used herein, the phrase “exogenous polynucleotide” refers to a heterologous nucleic acid sequence which is not naturally expressed within the plant (e.g., a nucleic acid sequence from a different species) or to an endogenous nucleic acid of which overexpression in the plant is desired. The exogenous polynucleotide may be introduced into the plant in a stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule and/or a polypeptide molecule. 
     The term “endogenous” as used herein refers to a polynucleotide or polypeptide which is naturally present and/or naturally expressed within a plant or a cell thereof. 
     The term “heterologous” as used herein refers to polynucleotide or polypeptide which is not naturally present and/or naturally expressed within a plant or a cell thereof. 
     According to one aspect, the present invention provides a method for enhancing the resistance of a plant or a part thereof to at least one pathogenic fungus and/or Oomycete, comprising modulating the expression and/or activity of at least one polypeptide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506 and 2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof within at least one cell of the plant or part thereof, thereby enhancing the resistance of said plant or part thereof to the at least one pathogenic fungus and/or Oomycetes compared to the resistance of a control plant. Each possibility represents a separate embodiment of the present invention. 
     According to another aspect, the present invention provides a method for enhancing the resistance of a plant or a part thereof to at least one pathogenic fungus and/or Oomycete, comprising modulating the expression of at least one polynucleotide encoding a polypeptide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2380-4175; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:339-537 and 2380-4175, a fragment and/or a variant thereof within at least one cell of the plant or part thereof, thereby enhancing the resistance of said plant or part thereof to the at least one pathogenic fungus and/or Oomycete compared to the resistance of a corresponding control plant. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the polypeptide is 70%-79% homologous to any one of the polypeptides set forth in SEQ ID NOs:2635, 2444-2634, 2636-4252. According to other embodiments, the polypeptide is 75%-79% homologous to any one of the polypeptides set forth in SEQ ID NOs:2635, 2444-2634, 2636-4252. 
     According to certain embodiments, the polypeptide is 80%-99% homologous to any one of the polypeptides set forth in SEQ ID NOs:326, 360, 308-325, 327-359, 361-506, 2635, 2444-2634, and 2636-4252. According to other embodiments, the polypeptide is 85%-95% homologous to any one of the polypeptides set forth in SEQ ID NOs: 326, 360, 308-325, 327-359, 361-506, 2635, 2444-2634, and 2636-4252. According to other embodiments, the polypeptide is 90%-99% homologous to any one of the polypeptides set forth in SEQ ID NOs:326, 360, 308-325, 327-359, 361-506, 2635, 2444-2634, and 2636-4252. According to certain embodiments, the polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 326, 360, 308-325, 327-359, 361-508, 2635, 2444-2634, and 2636-4252. Each possibility represents a separate embodiment of the present invention. According to certain embodiments, the polypeptide consists of the amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508 and 2635, 2444-2634, 2636-4252. Each possibility represents a separate embodiment of the present invention. 
     According to yet additional aspect, the present invention provides a method for enhancing the resistance of a plant or a part thereof to at least one pathogenic fungus and/or Oomycete, comprising modulating the expression of at least one polynucleotide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polynucleotide having a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to a polynucleotide having an nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, 81-301, 783, 592-782, 784-2443 within at least one cell of the plant or part thereof, thereby enhancing the resistance of said plant or part thereof to the at least one pathogenic fungus and/or Oomycetes compared to the resistance of a control plant. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the polynucleotide is 70%-79% homologous to any one of the polynucleotides set forth in SEQ ID NOs:783, 592-782, 784-2443. According to other embodiments, the polynucleotide is 75%-79% homologous to any one of the polynucleotides set forth in SEQ ID NOs:783, 592-782, 784-2443. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the polynucleotide is 80%-99% homologous to any one of the polynucleotides set forth in SEQ ID NOs:40, 80, 22-39, 41-79, 81-301, 783, 592-782, and 784-2443. According to other embodiments, the polynucleotide is 85%-95% homologous to any one of the polynucleotides set forth in SEQ ID NOs:40, 80, 22-39, 41-79, 81-301, 783, 592-782, and 784-2443. According to other embodiments, the polynucleotide is 90%-99% homologous to any one of the polynucleotides set forth in SEQ ID NOs:40, 80, 22-39, 41-79, 81-301, 783, 592-782, and 784-2443. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the polynucleotide comprises a nucleic acids sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-305, 783, 592-782, 784-2443. 
     According to other embodiments, the exogenous polynucleotide consists of a nucleic acids sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-305, 783, 592-782, and 784-2443. Each possibility represents a separate embodiment of the present invention. 
     According to another aspect, the present invention provides a method for producing a population of plants each having an enhanced resistance to at least one pathogenic fungus or Oomycete, comprising the steps of:
         (a) modulating the expression and/or activity of at least one polypeptide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506, 2635, 2444-2634, and 2636-4252, a fragment and/or a variant thereof within at least one cell of each plant of the plant population as to produce a genetically engineered plant population;   (b) inoculating each plant of the genetically engineered plant population with the at least one pathogenic fungus; and   (c) selecting plants showing an enhanced resistance to said at least one pathogenic fungus compared to a control or to a pre-determined resistance score value;       

     thereby producing a population of genetically engineered plants having enhanced resistance to said at least one pathogenic fungus. 
     According to certain embodiments, the method comprises modulating the expression and/or activity of a polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508, 2635, 2444-2634, and 2636-4252. According to other embodiments, the method comprises enhancing the expression and/or activity of a polypeptide having the amino acid sequence set forth in SEQ ID NO:509. According to yet additional embodiments, the method comprises enhancing the expression and/or activity of a polypeptide consisting of the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-509, 2635, 2444-2634, and 2636-4252. 
     According to additional aspect, the present invention provides a method for selecting a plant having an enhanced resistance to at least one pathogenic fungus and/or Oomycete, comprising the steps of:
         (a) providing a plurality of plants each comprising at least one cell with modulated expression and/or activity of a polypeptide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506, 2635, 2444-2634, and 2636-4252, a fragment and/or a variant thereof;   (b) inoculating the plurality of plants with the at least one pathogenic fungus or Oomycete; and   (c) selecting plants showing an enhanced resistance to said at least one pathogenic fungus or Oomycete compared to a control plant or to a pre-determined resistance score value.       

     According to certain embodiments, the method comprises providing a plurality of plants each comprising at least one cell with modulated expression and/or activity of at least one polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508, 2635, 2444-2634, and 2636-4252. Each possibility represents a separate embodiment of the present invention. According to other embodiments, the method comprises providing a plurality of plants each having modulated expression and/or activity of a polypeptide having the amino acid sequence set forth SEQ ID NO:509. According to other embodiments, the method comprises providing a plurality of plants each having modulated expression and/or activity of at least one polypeptide consisting of the amino acid sequence set forth in any one of SEQ ID NOs: 326, 360, 308-325, 327-359, 361-509, 2635, 2444-2634, and 2636-4252. 
     The plurality of plants having modulated expression and/or activity of the polypeptide may include plants having at least one cell with enhanced expression and/or activity of a polypeptide, plants having at least one cell with reduced expression and/or activity of a polypeptide or a combination thereof. Enhancing or reducing the expression and/or activity of the polypeptide can be performed as is known in the Art and as described hereinbelow. 
     According to certain embodiments, the polypeptide the expression and/or activity of which is to be enhanced comprises an amino acid sequence at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:2635, 2444-2634, 2636-4252 or at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-506. According to some embodiments, the polypeptide the expression and/or activity of which is to be enhanced comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-508, 2635, 2444-2634, and 2636-4252. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, enhancing the resistance of the plant to the pathogenic fungus and/or Oomycete comprises reducing the expression and/or activity of at least one polypeptide compared to its expression and/or activity in the control plant. According to certain exemplary embodiments, the polypeptide the expression and/or activity of which is to be reduced comprises an amino acid sequence at least 80% identical to the amino acid sequence set forth in any one of SEQ ID NO:328, SEQ ID NO:361, and SEQ ID NO:348. According to further exemplary embodiments, the polypeptide the expression and/or activity of which is to be reduced comprises the amino acid sequence set forth in any one of SEQ ID NO:328, SEQ ID NO:361 and SEQ ID NO:348. 
     According to another aspect, the present invention provides a method for conferring and/or enhancing the resistance of a grafted plant to at least one pathogenic fungus and/or Oomycete, the method comprising providing a scion and a rootstock, wherein the rootstock exhibits enhanced resistance to the at least one pathogenic fungus and/or Oomycete, said rootstock comprises at least one cell with a modulated expression of a polynucleotide encoding a polypeptide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous, or identical to the amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506, 2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof compared to the scion and grafting said scion onto said rootstock, thereby producing a grafted plant having an enhanced resistance to said at least one pathogenic fungus and/or Oomycete. 
     According to another aspect, the present invention provides a method for conferring and/or enhancing the resistance of a grafted plant to at least one pathogenic fungus and/or Oomycete, the method comprising providing a scion and a rootstock, the scion having an enhances resistance to the at least one pathogenic fungus and/or Oomycete, said scion comprises at least one cell with modulated expression of at least one polynucleotide encoding a polypeptide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous, or identical to the amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506, 2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof compared to the rootstock, and grafting said scion onto said rootstock, thereby producing a grafted plant having an enhanced resistance to the at least one pathogenic fungus and/or Oomycete. 
     According to certain embodiments, the polypeptide expressed in the scion or in the rootstock comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508, 2635, 2444-2634, and 2636-4252. 
     According to certain embodiments, the scion or rootstock having enhanced resistance to the at least one fungus and/or Oomycete comprises at least one cell with enhanced expression and/or activity of at least one polypeptide or the nucleotide encoding same. According to other embodiments, the scion or rootstock having enhanced resistance to the at least one fungus and/or Oomycete comprises at least one cell with reduced expression and/or activity of at least one polypeptide or the nucleotide encoding same. The polypeptides the expression of which is enhanced or reduced are as described hereinabove. 
     According to some embodiments, the at least one polynucleotide is constitutively expressed in the transgenic rootstock. According to some embodiments, the at least one polynucleotide is expressed in the transgenic rootstock in a tissue specific or inducible manner. According to some embodiments, the expression of the at least one polynucleotide is induced by biotic stress, particularly by fungi infection. 
     According to additional aspect, the present invention provides a method of growing a crop plant having enhanced resistance to at least one pathogenic fungus and/or Oomycete comprising the steps of:
         (a) selecting a parent plant having a modulated expression of at least one polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous, or identical to the amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506, 2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof for enhanced resistance to at least one pathogenic fungus and/or Oomycete; and   (b) growing a progeny crop plant of the parent plant, wherein the progeny crop plant having modulated expression of the polynucleotide has an enhanced resistance to the at least one pathogenic fungus and/or Oomycete.       

     According to certain embodiments, the encoded polypeptide comprises the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508, 2635, 2444-2634, and 2636-4252. According to certain embodiments, the encoded polypeptide comprises the amino acid sequence set forth in SEQ ID NOs:5509. According to some embodiments, the encoded polypeptide consists of the amino acid sequence set forth in any one of SEQ ID NOs: 326, 360, 308-325, 327-359, 361-509, 2635, 2444-2634, 2636-4252. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the polynucleotide comprises a nucleic acid sequence at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polynucleotide having a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to a nucleic acid sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-301, 783, 592-782, and 784-2443. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the polynucleotide comprises the nucleic acid sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-305, 783, 592-782, and 784-2443. According to certain embodiments, the polynucleotide comprises the nucleic acid sequence set forth in SEQ ID NO:306. According to some embodiments, the polynucleotide consists of the nucleic acid sequence set forth in any one of SEQ ID NOs: 40, 80, 22-39, 41-79, 81-306, 783, 592-782, 784-2443. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the modulated expression comprises up-regulation of at least one polynucleotide expression. According to certain embodiments, the modulated expression comprises down-regulation of at least one polynucleotide expression. 
     According to certain exemplary embodiments, the gene to be upregulated comprises a nucleic acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to a nucleic acid sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41, 43-61, 63-79, 81, 83-301. According to some embodiments, the gene to be upregulated comprises a polynucleotide selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41, 43-61, 63-79, 81, 83-306. 
     According to certain exemplary embodiments, the gene the expression of which is to be downregulated comprises a nucleic acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to a nucleic acid sequence set forth in any one of SEQ ID NOs:42, 62 and 82. According to certain embodiments, the gene the expression of which is to be downregulated comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:42, 62 and 82. 
     According to certain embodiments, the encoded polypeptide the expression and/or activity of which is to be downregulated is at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to an amino acid sequence set forth in any one of SEQ ID NOs:328, 248 and 361. According to certain embodiments, the polypeptide the expression and/or activity of which is to be downregulated comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:328, 248 and 361. Each possibility represents a separate embodiment of the present invention. 
     According to yet additional aspect, the present invention provides a method of producing seeds of a crop comprising the steps of:
         (a) selecting a parent plant having a modulated expression of at least one polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous, or identical to the amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506, 2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof for enhanced resistance to at least one pathogenic fungus and/or Oomycete;   (b) growing the selected parent plant of step (a) to produce seeds;   (c) harvesting the produced seeds.       

     According to certain embodiments, the encoded polypeptide comprises the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508 and 2635, 2444-2634, 2636-4252. According to certain embodiments, the encoded polypeptide comprises the amino acid sequence set forth in SEQ ID NO:509. According to some embodiments, the encoded polypeptide consists of the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508 and 2635, 2444-2634, 2636-4252. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the modulated expression of the least one polynucleotide comprises up-regulation of a polynucleotide expression as described hereinabove. According to certain embodiments, the modulated expression of the least one polynucleotide comprises down-regulation of a polynucleotide expression as described hereinabove. 
     According to certain embodiments, the seeds produced by the methods of the invention comprise at least one cell having modulated expression of the polynucleotide. According to some embodiments, plants grown from the produced seeds have enhanced resistance to at least one pathogenic fungus and/or Oomycete. 
     The present invention encompasses polynucleotides identified to be associated with resistance to at least one pathogenic fungus and/or Oomycete polypeptides encoded by same and homologs thereto. 
     According to certain embodiments, the exogenous isolated or recombinant polynucleotides employed in the methods of the present invention encode a polypeptide comprising an amino acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to an amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-509 and 2635, 2444-2634, 2636-4252. 
     According to certain embodiments, the exogenous isolated or recombinant polynucleotides employed in the methods of the present invention comprise a nucleic acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous to, or identical to a nucleic acid sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-306 and 783, 592-782, 784-2443. 
     Homologous sequences include both orthologous and paralogous sequences. The term “paralogous” relates to gene-duplications within the genome of a species leading to paralogous genes. The term “orthologous” relates to homologous genes in different organisms due to ancestral relationship. Thus, orthologs are evolutionary counterparts derived from a single ancestral gene in the last common ancestor of given two species (Koonin E V and Galperin M Y 2003. Sequence—Evolution—Function: Computational Approaches in Comparative Genomics. Boston: Kluwer Academic; Chapter 2, Evolutionary Concept in Genetics and Genomics) and therefore have great likelihood of having the same function. 
     One option to identify orthologues in monocot or in dicot plant species is by performing a reciprocal blast search. This may be done by a first blast involving blasting the sequence-of-interest against any sequence database, such as the publicly available NCBI database which may be found at: ncbi.nlm.nih.gov. If orthologues in rice were sought, the sequence-of-interest would be blasted against, for example, the 28,469 full-length cDNA clones from  Oryza sativa  Nipponbare available at NCBI. The blast results may be filtered. The full-length sequences of either the filtered results or the non-filtered results are then blasted back (second blast) against the sequences of the organism from which the sequence-of-interest is derived. The results of the first and second blasts are then compared. An ortholog is identified when the sequence resulting in the highest score (best hit) in the first blast identifies in the second blast the query sequence (the original sequence-of-interest) as the best hit. Using the same rational a paralog (homolog to a gene in the same organism) is found. In case of large sequence families, the ClustalW program may be used (ebi.ac.uk/Tools/clustalw2/index.html), followed by a neighbor-joining tree (Wikipedia.org/wiki/Neighbor-joining) which helps visualizing the clustering. 
     Homology (e.g., percent homology, sequence identity+sequence similarity) can be determined using any homology comparison software computing a pairwise sequence alignment. 
     As used herein, “sequence identity” or “identity” in the context of two nucleic acid or polypeptide sequences includes reference to the residues in the two sequences which are the same when aligned. When percentage of sequence identity is used in reference to proteins it is recognized that residue positions which are not identical often differ by conservative amino acid substitutions, where amino acid residues are substituted for other amino acid residues with similar chemical properties (e.g. charge or hydrophobicity) and therefore do not change the functional properties of the molecule. Where sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Sequences which differ by such conservative substitutions are considered to have “sequence similarity” or “similarity”. Means for making this adjustment are well-known to those of skill in the art. Typically this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated, e.g., according to the algorithm of Henikoff S and Henikoff J G. (Amino acid substitution matrices from protein blocks. Proc. Natl. Acad. Sci. U.S.A. 1992, 89(22): 10915-9). 
     Identity (e.g., percent homology) can be determined using any homology comparison software, including for example, the BlastN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters. 
     According to some embodiments of the invention, the identity is a global identity, i.e., an identity over the entire amino acid or nucleic acid sequences of the invention and not over portions thereof. 
     According to some embodiments of the invention, the term “homology” or “homologous” refers to identity of two or more nucleic acid sequences; or identity of two or more amino acid sequences; or the identity of an amino acid sequence to one or more nucleic acid sequence. 
     According to some embodiments of the invention, the homology is a global homology, i.e., a homology over the entire amino acid or nucleic acid sequences of the invention and not over portions thereof. 
     According to some embodiments of the invention, the homology is a partial homology, i.e. a homology over part of the amino acid or nucleic acid sequences of the invention. According to certain exemplary embodiments, the partial sequence is of polynucleotides and polypeptides of embodiments of the present invention lacking a signal or transit peptide. 
     The degree of homology or identity between two or more sequences can be determined using various known sequence comparison tools. Following is a non-limiting description of such tools which can be used along with some embodiments of the invention. 
     Pairwise global alignment was defined by S. B. Needleman and C. D. Wunsch, “A general method applicable to the search of similarities in the amino acid sequence of two proteins” Journal of Molecular Biology, 1970, pages 443-53, volume 48). 
     For example, when starting from a polypeptide sequence and comparing to other polypeptide sequences, the EMBOSS-6.0.1 Needleman-Wunsch algorithm (available from emboss(dot)sourceforge(dot)net/apps/cvs/emboss/apps/needle(dot)html) can be used to find the optimum alignment (including gaps) of two sequences along their entire length—a “Global alignment”. Default parameters for Needleman-Wunsch algorithm (EMBOSS-6.0.1) include: gapopen=10; gapextend=0.5; datafile=EBLOSUM62; brief=YES. 
     According to some embodiments of the invention, the parameters used with the EMBOSS-6.0.1 tool (for protein-protein comparison) include: gapopen=8; gapextend=2; datafile=EBLOSUM62; brief=YES. 
     According to some embodiments of the invention, the threshold used to determine homology using the EMBOSS-6.0.1 Needleman-Wunsch algorithm is 80%, 81%, 82%, 83%, 84%, 8%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 9%, 96%, 97%, 98%, 99%, or 100%. 
     When starting from a polypeptide sequence and comparing to polynucleotide sequences, the OneModel FramePlus algorithm [Halperin, E., Faigler, S. and Gill-More, R. (1999) —FramePlus: aligning DNA to protein sequences. Bioinformatics, 15, 867-873) (available from biocceleration(dot)com/Products(dot)html] can be used with following default parameters: model=frame+_p2n.model mode=local. 
     According to some embodiments of the invention, the parameters used with the OneModel FramePlus algorithm are model=frame+_p2n.model, mode=qglobal. 
     According to some embodiments of the invention, the threshold used to determine homology using the OneModel FramePlus algorithm is 80%, 81%, 82%, 83%, 84%, 85%, 86%, 8%, 88%, 89%, 90%, 91%, 9%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%. 
     When starting with a polynucleotide sequence and comparing to other polynucleotide sequences the EMBOSS-6.0.1 Needleman-Wunsch algorithm (available from emboss(dot)sourceforge(dot)net/apps/cvs/emboss/apps/needle(dot)html) can be used with the following default parameters: (EMBOSS-6.0.1) gapopen=10; gapextend=0.5; datafile=EDNAFULL; brief=YES. 
     According to some embodiments of the invention, the parameters used with the EMBOSS-6.0.1 Needleman-Wunsch algorithm are gapopen=10; gapextend=0.2; datafile=EDNAFULL; brief=YES. 
     According to some embodiments of the invention, the threshold used to determine homology using the EMBOSS-6.0.1 Needleman-Wunsch algorithm for comparison of polynucleotides with polynucleotides is 80%, 81%, 82%, 83%, 84%, 85%, 86%, 8%, 88%, 89%, 90%, 91%, 9%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%. 
     According to some embodiment, determination of the degree of homology further requires employing the Smith-Waterman algorithm (for protein-protein comparison or nucleotide-nucleotide comparison). 
     Default parameters for GenCore 6.0 Smith-Waterman algorithm include: model=sw.model. 
     According to some embodiments of the invention, the threshold used to determine homology using the Smith-Waterman algorithm is 80%, 81%, 82%, 83%, 84%, 85%, 86%, 8%, 88%, 89%, 90%, 91%, 9%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%. 
     According to some embodiments of the invention, the global homology is performed on sequences which are pre-selected by local homology to the polypeptide or polynucleotide of interest (e.g., 60% identity over 60% of the sequence length), prior to performing the global homology to the polypeptide or polynucleotide of interest (e.g., 80% global homology on the entire sequence). For example, homologous sequences are selected using the BLAST software with the Blastp and tBlastn algorithms as filters for the first stage, and the needle (EMBOSS package) or Frame+ algorithm alignment for the second stage. Local identity (Blast alignments) is defined with a very permissive cutoff—60% Identity on a span of 60% of the sequences lengths because it is used only as a filter for the global alignment stage. In this specific embodiment (when the local identity is used), the default filtering of the Blast package is not utilized (by setting the parameter “-F F”). 
     In the second stage, homologs are defined based on a global identity of at least 80% to the core gene polypeptide sequence. 
     According to some embodiments of the invention, two distinct forms for finding the optimal global alignment for protein or nucleotide sequences are used: 
     1. Between Two Proteins (Following the Blastp Filter): 
     EMBOSS-6.0.1 Needleman-Wunsch algorithm with the following modified parameters: gapopen=8 gapextend=2. The rest of the parameters are unchanged from the default options listed here: 
     Standard (Mandatory) qualifiers: 
     
       
         
           
               
               
               
             
               
                   
               
             
            
               
                 [-asequence] 
                 sequence 
                 filename and optional format, or reference 
               
               
                   
                   
                 (input USA) 
               
               
                 [-bsequence] 
                 seqall 
                 Sequence(s) filename and optional format, or 
               
               
                   
                   
                 reference (input USA) 
               
               
                   
               
            
           
         
       
     
     -gapopen float [10.0 for any sequence]. The gap open penalty is the score taken away when a gap is created. The best value depends on the choice of comparison matrix. The default value assumes you are using the EBLOSUM62 matrix for protein sequences, and the EDNAFULL matrix for nucleotide sequences. (Floating point number from 1.0 to 100.0) 
     -gapextend float [0.5 for any sequence]. The gap extension, penalty is added to the standard gap penalty for each base or residue in the gap. This is how long gaps are penalized. Usually you will expect a few long gaps rather than many short gaps, so the gap extension penalty should be lower than the gap penalty. An exception is where one or both sequences are single reads with possible sequencing errors in which case you would expect many single base gaps. You can get this result by setting the gap open penalty to zero (or very low) and using the gap extension penalty to control gap scoring. (Floating point number from 0.0 to 10.0) 
     [-outfile] align [*needle] Output alignment file name 
     Additional (Optional) qualifiers: 
     -datafile matrixf [EBLOSUM62 for protein, EDNAFULL for DNA]. This is the scoring matrix file used when comparing sequences. By default, it is the file ‘EBLOSUM62’ (for proteins) or the file ‘EDNAFULL’ (for nucleic sequences). These files are found in the ‘data’ directory of the EMBOSS installation. 
     Advanced (Unprompted) qualifiers: 
     -[no]brief boolean [Y] Brief identity and similarity 
     Associated qualifiers: 
     
       
         
           
               
             
               
                   
               
             
            
               
                 “-asequence” associated qualifiers 
               
            
           
           
               
               
               
               
            
               
                   
                 -sbegin1 
                 integer 
                 Start of the sequence to be used 
               
               
                   
                 -send1 
                 integer 
                 End of the sequence to be used 
               
               
                   
                 -sreverse1 
                 boolean 
                 Reverse (if DNA) 
               
               
                   
                 -sask1 
                 boolean 
                 Ask for begin/end/reverse 
               
               
                   
                 -snucleotide1 
                 boolean 
                 Sequence is nucleotide 
               
               
                   
                 -sprotein1 
                 boolean 
                 Sequence is protein 
               
               
                   
                 -slower1 
                 boolean 
                 Make lower case 
               
               
                   
                 -supper1 
                 boolean 
                 Make upper case 
               
               
                   
                 -sformat1 
                 string 
                 Input sequence format 
               
               
                   
                 -sdbname1 
                 string 
                 Database name 
               
               
                   
                 -sid1 
                 string 
                 Entryname 
               
               
                   
                 -ufo1 
                 string 
                 UFO features 
               
               
                   
                 -fformat1 
                 string 
                 Features format 
               
               
                   
                 -fopenfile1 
                 string 
                 Features file name 
               
            
           
           
               
            
               
                 “-bsequence” associated qualifiers 
               
            
           
           
               
               
               
               
            
               
                   
                 -sbegin2 
                 integer 
                 Start of each sequence to be used 
               
               
                   
                 -send2 
                 integer 
                 End of each sequence to be used 
               
               
                   
                 -sreverse2 
                 boolean 
                 Reverse (if DNA) 
               
               
                   
                 -sask2 
                 boolean 
                 Ask for begin/end/reverse 
               
               
                   
                 -snucleotide2 
                 boolean 
                 Sequence is nucleotide 
               
               
                   
                 -sprotein2 
                 boolean 
                 Sequence is protein 
               
               
                   
                 -slower2 
                 boolean 
                 Make lower case 
               
               
                   
                 -supper2 
                 boolean 
                 Make upper case 
               
               
                   
                 -sformat2 
                 string 
                 Input sequence format 
               
               
                   
                 -sdbname2 
                 string 
                 Database name 
               
               
                   
                 -sid2 
                 string 
                 Entryname 
               
               
                   
                 -ufo2 
                 string 
                 UFO features 
               
               
                   
                 -fformat2 
                 string 
                 Features format 
               
               
                   
                 -fopenfile2 
                 string 
                 Features file name 
               
            
           
           
               
            
               
                 “-outfile” associated qualifiers 
               
            
           
           
               
               
               
               
            
               
                   
                 -aformat3 
                 string 
                 Alignment format 
               
               
                   
                 -aextension3 
                 string 
                 File name extension 
               
               
                   
                 -adirectory3 
                 string 
                 Output directory 
               
               
                   
                 -aname3 
                 string 
                 Base file name 
               
               
                   
                 -awidth3 
                 integer 
                 Alignment width 
               
               
                   
                 -aaccshow3 
                 boolean 
                 Show accession number in the header 
               
               
                   
                 -adesshow3 
                 boolean 
                 Show description in the header 
               
               
                   
                 -ausashow3 
                 boolean 
                 Show the full USA in the alignment 
               
               
                   
                 -aglobal3 
                 boolean 
                 Show the full sequence in alignment 
               
               
                   
                   
               
            
           
         
       
     
     General qualifiers: 
     
       
         
           
               
               
               
             
               
                   
               
             
            
               
                 -auto 
                 boolean 
                 Turn off prompts 
               
               
                 -stdout 
                 boolean 
                 Write first file to standard output 
               
               
                 -filter 
                 boolean 
                 Read first file from standard input, write 
               
               
                   
                   
                 first file to standard output 
               
               
                 -options 
                 boolean 
                 Prompt for standard and additional values 
               
               
                 -debug 
                 boolean 
                 Write debug output to program.dbg 
               
               
                 -verbose 
                 boolean 
                 Report some/full command line options 
               
               
                 -help 
                 boolean Re 
                 port command line options. More information 
               
               
                   
                   
                 on associated and general qualifiers can be 
               
               
                   
                   
                 found with -help -verbose 
               
               
                 -warning 
                 boolean 
                 Report warnings 
               
               
                 -error 
                 boolean 
                 Report errors 
               
               
                 -fatal 
                 boolean 
                 Report fatal errors 
               
               
                 -die 
                 boolean 
                 Report dying program messages 
               
               
                   
               
            
           
         
       
     
     2. Between a Protein Sequence and a Nucleotide Sequence (Following the Tblastn Filter): 
     GenCore 6.0 OneModel application utilizing the Frame+ algorithm with the following parameters: model=frame+_p2n.model mode=qglobal -q=protein. sequence -db=nucleotide. sequence. The rest of the parameters are unchanged from the default options: 
     Usage: 
     om -model=&lt;model_fname&gt; [-q=]query [-db=]database [options] 
     -model=&lt;model_fname&gt; Specifies the model that you want to run. All models supplied by Compugen are located in the directory $CGNROOT/models/. 
     Valid command line parameters: 
     -dev=&lt;dev name&gt; Selects the device to be used by the application. 
     Valid devices are: 
     bic—Bioccelerator (valid for SW, XSW, FRAME_N2P, and FRAME_P2N models). 
     xlg—BioXL/G (valid for all models except XSW). 
     xlp—BioXL/P (valid for SW, FRAME+N2P, and 
     FRAME_P2N models). 
     xlh—BioXL/H (valid for SW, FRAME+N2P, and 
     FRAME_P2N models). 
     soft—Software device (for all models). 
     -q=&lt;query&gt; Defines the query set. The query can be a sequence file or a database reference. You can specify a query by its name or by accession number. The format is detected automatically. However, you may specify a format using the -qfmt parameter. If you do not specify a query, the program prompts for one. If the query set is a database reference, an output file is produced for each sequence in the query. 
     -db=&lt;database name&gt; Chooses the database set. The database set can be a sequence file or a database reference. The database format is detected automatically. However, you may specify a format using -dfmt parameter. 
     -qacc Add this parameter to the command line if you specify query using accession numbers. 
     -dacc Add this parameter to the command line if you specify a database using accession numbers. 
     -dfmt/-qfmt=&lt;format_type&gt; Chooses the database/query format type. Possible formats are: 
     fasta—fasta with seq type auto-detected. 
     fastap—fasta protein seq. 
     fastan—fasta nucleic seq. 
     gcg—gcg format, type is auto-detected. 
     gcg9seq—gcg9 format, type is auto-detected. 
     gcg9seqp—gcg9 format protein seq. 
     gcg9seqn—gcg9 format nucleic seq. 
     nbrf—nbrf seq, type is auto-detected. 
     nbrfp—nbrf protein seq. 
     nbrfn—nbrf nucleic seq. 
     embl—embl and swissprot format. 
     genbank—genbank format (nucleic). 
     blast—blast format. 
     nbrf_gcg—nbrf-gcg seq, type is auto-detected. 
     nbrf_gcgp—nbrf-gcg protein seq. 
     nbrf_gcgn—nbrf-gcg nucleic seq. 
     raw—raw ascii sequence, type is auto-detected. 
     rawp—raw ascii protein sequence. 
     rawn—raw ascii nucleic sequence. 
     pir—pir codata format, type is auto-detected. 
     profile—gcg profile (valid only for -qfmt 
     in SW, XSW, FRAME_P2N, and FRAME+_P2N). 
     -out=&lt;out_fname&gt; The name of the output file. 
     -suffix=&lt;name&gt; The output file name suffix. 
     -gapop=&lt;n&gt; Gap open penalty. This parameter is not valid for FRAME+. For FrameSearch the default is 12.0. For other searches the default is 10.0. 
     -gapext=&lt;n&gt; Gap extend penalty. This parameter is not valid for FRAME+. For FrameSearch the default is 4.0. For other models: the default for protein searches is 0.05, and the default for nucleic searches is 1.0. 
     -qgapop=&lt;n&gt; The penalty for opening a gap in the query sequence. The default is 10.0. Valid for XSW. 
     -qgapext=&lt;n&gt; The penalty for extending a gap in the query sequence. The default is 0.05. Valid for XSW. 
     -start=&lt;n&gt; The position in the query sequence to begin the search. 
     -end=&lt;n&gt; The position in the query sequence to stop the search. 
     -qtrans Performs a translated search, relevant for a nucleic query against a protein database. The nucleic query is translated to six reading frames and a result is given for each frame. 
     Valid for SW and XSW. 
     -dtrans Performs a translated search, relevant for a protein query against a DNA database. Each database entry is translated to six reading frames and a result is given for each frame. 
     Valid for SW and XSW. 
     Note: “-qtrans” and “-dtrans” options are mutually exclusive. 
     -matrix=&lt;matrix file&gt; Specifies the comparison matrix to be used in the search. The matrix must be in the BLAST format. If the matrix file is not located in $CGNROOT/tables/matrix, specify the full path as the value of the -matrix parameter. 
     -trans=&lt;transtab_name&gt; Translation table. The default location for the table is $CGNROOT/tables/trans. 
     -onestrand Restricts the search to just the top strand of the query/database nucleic sequence. 
     -list=&lt;n&gt; The maximum size of the output hit list. The default is 50. 
     -docalign=&lt;n&gt; The number of documentation lines preceding each alignment. The default is 10. 
     -thr_score=&lt;score_name&gt; The score that places limits on the display of results. Scores that are smaller than -thr_min value or larger than -thr_max value are not shown. Valid options are: quality. 
     zscore. 
     escore. 
     -thr_max=&lt;n&gt; The score upper threshold. Results that are larger than -thr_max value are not shown. 
     -thr_min=&lt;n&gt; The score lower threshold. Results that are lower than -thr_min value are not shown. 
     -align=&lt;n&gt; The number of alignments reported in the output file. 
     -noalign Do not display alignment. 
     Note: “-align” and “-noalign” parameters are mutually exclusive. 
     -outfmt=&lt;format_name&gt; Specifies the output format type. The default format is PFS. Possible values are: 
     PFS—PFS text format 
     FASTA—FASTA text format 
     BLAST—BLAST text format 
     -nonorm Do not perform score normalization. 
     -norm=&lt;norm_name&gt; Specifies the normalization method. Valid options are: 
     log—logarithm normalization. 
     std—standard normalization. 
     stat—Pearson statistical method. 
     Note: “-nonorm” and “-norm” parameters cannot be used together. 
     Note: Parameters -xgapop, -xgapext, -fgapop, -fgapext, -ygapop, -ygapext, -delop, and -delext apply only to FRAME+. 
     -xgapop=&lt;n&gt; The penalty for opening a gap when inserting a codon (triplet). The default is 12.0. 
     -xgapext=&lt;n&gt; The penalty for extending a gap when inserting a codon (triplet). The default is 4.0. 
     -ygapop=&lt;n&gt; The penalty for opening a gap when deleting an amino acid. The default is 12.0. 
     -ygapext=&lt;n&gt; The penalty for extending a gap when deleting an amino acid. The default is 4.0. 
     -fgapop=&lt;n&gt; The penalty for opening a gap when inserting a DNA base. The default is 6.0. 
     -fgapext=&lt;n&gt; The penalty for extending a gap when inserting a DNA base. The default is 7.0. 
     -delop=&lt;n&gt; The penalty for opening a gap when deleting a DNA base. The default is 6.0. 
     -delext=&lt;n&gt; The penalty for extending a gap when deleting a DNA base. The default is 7.0. 
     -silent No screen output is produced. 
     -host=&lt;host name&gt; The name of the host on which the server runs. By default, the application uses the host specified in the file $CGNROOT/cgnhosts. 
     -wait Do not go to the background when the device is busy. This option is not relevant for the Parseq or Soft pseudo device. 
     -batch Run the job in the background. When this option is specified, the file “$CGNROOT/defaults/batch.defaults” is used for choosing the batch command. If this file does not exist, the command “at now” is used to run the job. 
     Note: “-batch” and “-wait” parameters are mutually exclusive. 
     -version Prints the software version number. 
     -help Displays this help message. To get more specific help type:
         “om -model=&lt;model_fname&gt;-help”.       

     According to some embodiments the homology is a local homology or a local identity. 
     Local alignments tools include, but are not limited to the BlastP, BlastN, BlastX or TBLASTN software of the National Center of Biotechnology Information (NCBI), FASTA, and the Smith-Waterman algorithm. 
     A tblastn search allows the comparison between a protein sequence to the six-frame translations of a nucleotide database. It can be a very productive way of finding homologous protein coding regions in unannotated nucleotide sequences such as expressed sequence tags (ESTs) and draft genome records (HTG), located in the BLAST databases est and htgs, respectively. 
     Default parameters for blastp include: Max target sequences: 100; Expected threshold: e-5; Word size: 3; Max matches in a query range: 0; Scoring parameters: Matrix—BLOSUM62; filters and masking: Filter—low complexity regions. 
     Local alignments tools, which can be used include, but are not limited to, the tBLASTX algorithm, which compares the six-frame conceptual translation products of a nucleotide query sequence (both strands) against a protein sequence database. Default parameters include: Max target sequences: 100; Expected threshold: 10; Word size: 3; Max matches in a query range: 0; Scoring parameters: Matrix—BLOSUM62; filters and masking: Filter—low complexity regions. 
     Modulating the expression and/or activity of the polypeptides of the present invention within a plant cell as to enhance the resistance of the plant to the pathogenic fungi and/or Oomycetes may include enhancing the expression and/or activity of polypeptides identified to positively contribute to the plant defense mechanism against the pathogenic fungi, or reducing the expression and/or activity of those polypeptides found to be associated with susceptibility to the fungus or Oomycete infection. 
     According to certain embodiments, enhancing the resistance of the plant or part thereof to the pathogenic fungus and/or Oomycete comprises enhancing the expression and/or activity of the at least one polypeptide compared to its expression and/or activity in the control plant. 
     According to certain embodiments, enhancing the expression and/or activity of the polypeptide comprises introducing into at least one cell of the plant or part thereof an exogenous polynucleotide encoding said polypeptide, thereby producing a transgenic plant over-expressing said polypeptide compared to the control plant. 
     According to certain embodiments, the exogenous polynucleotide encodes a polypeptide endogenous to the at least one cell. According to other embodiments, the exogenous polynucleotide encodes a polypeptide heterologous to the at least one plant cell. 
     Nucleic acid sequences encoding the polypeptides of the present invention may be optimized for expression. Examples of such sequence modifications include, but are not limited to, an altered G/C content to more closely approach that typically found in the plant species of interest, and the removal of codons atypically found in the plant species commonly referred to as codon optimization. 
     The phrase “codon optimization” refers to the selection of appropriate DNA nucleotides for use within a structural gene or fragment thereof that approaches codon usage within the plant of interest, and/or to a process of modifying a nucleic acid sequence for enhanced expression in the host cells of interest by replacing at least one codon (e.g., about or more than about 1, 2, 3, 4, 5, 10, 15, 20, 25, 50, or more codons) of the native sequence with codons that are more frequently or most frequently used in the genes of that host cell while maintaining the native amino acid sequence. Various species exhibit particular bias for certain codons of a particular amino acid. Codon bias (differences in codon usage between organisms) often correlates with the efficiency of translation of messenger RNA (mRNA), which is in turn believed to be dependent on, among other things, the properties of the codons being translated and the availability of particular transfer RNA (tRNA) molecules. The predominance of selected tRNAs in a cell is generally a reflection of the codons used most frequently in peptide synthesis. Accordingly, genes can be tailored for optimal gene expression in a given organism based on codon optimization. Therefore, an optimized gene or nucleic acid sequence refers to a gene in which the nucleotide sequence of a native or naturally occurring gene has been modified in order to utilize statistically-preferred or statistically-favored codons within the plant. The nucleotide sequence typically is examined at the DNA level and the coding region optimized for expression in the plant species determined using any suitable procedure. For example (see U.S. Pat. No. 7,214,862), the standard deviation of codon usage (SDCU), a measure of codon usage bias, may be calculated by first finding the squared proportional deviation of usage of each codon of the native gene relative to that of highly expressed plant genes, followed by a calculation of the average squared deviation. The formula used is: 
     
       
         
           
             
               ∑ 
               
                 n 
                 = 
                 1 
               
               N 
             
              
             
               
                 [ 
                 
                   
                     ( 
                     
                       
                         X 
                         n 
                       
                       - 
                       
                         Y 
                         n 
                       
                     
                     ) 
                   
                   / 
                   
                     Y 
                     n 
                   
                 
                 ] 
               
                
               
                 2 
                 / 
                 N 
               
             
           
         
       
     
     wherein Xn refers to the frequency of usage of codon n in highly expressed plant genes, where Yn to the frequency of usage of codon n in the gene of interest and N refers to the total number of codons in the gene of interest. A Table of codon usage from highly expressed genes of dicotyledonous plants is compiled using the data of Murray et al. (1989, Nuc Acids Res. 17:477-498). 
     Alternative method of optimizing the nucleic acid sequence in accordance with the preferred codon usage for a particular plant cell type is based on the direct use, without performing any extra statistical calculations, of codon optimization Tables such as those provided on-line at the Codon Usage Database through the NIAS (National Institute of Agrobiological Sciences) DNA bank in Japan (www.kazusa.or.jp/codon/). The Codon Usage Database contains codon usage tables for a number of different species, with each codon usage Table having been statistically determined based on the data present in Genbank. 
     By using the tables described above to determine the most preferred or most favored codons for each amino acid in a particular species (for example, rice), a naturally-occurring nucleotide sequence encoding a protein of interest can be codon optimized for that particular plant species. This is affected by replacing codons that may have a low statistical incidence in the particular species genome with corresponding codons, in regard to an amino acid, that are statistically more favored. However, one or more less-favored codons may be selected to delete existing restriction sites, to create new ones at potentially useful junctions (5′ and 3′ ends to add signal peptide or termination cassettes, internal sites that might be used to cut and splice segments together to produce a correct full-length sequence), or to eliminate nucleotide sequences that may negatively affect mRNA stability or expression. 
     The naturally-occurring encoding nucleotide sequence may already, in advance of any modification, contain a number of codons that correspond to a statistically-favored codon in a particular plant species. Therefore, codon optimization of the native nucleotide sequence may comprise determining which codons, within the native nucleotide sequence, are not statistically-favored with regards to a particular plant, and modifying these codons in accordance with a codon usage table of the particular plant to produce a codon optimized derivative. A modified nucleotide sequence may be fully or partially optimized for plant codon usage provided that the protein encoded by the modified nucleotide sequence is produced at a level higher than the protein encoded by the corresponding naturally occurring or native gene. Construction of synthetic genes by altering the codon usage is described in for example PCT Patent Application Publication No. WO 93/07278. 
     Bacterial genes quite often possess multiple methionine initiation codons in proximity to the start of the open reading frame. Translation initiation at one or more of these start codons often leads to generation of a functional protein, and it is not always predetermined which of these codons are used naturally in the bacterium. These start codons can include ATG codons, but additional codons, such GTG, may be used, for example by  Bacillus  sp. as a start codon, and proteins that initiate translation at GTG codons contain a methionine at the first amino acid. Thus, it is understood that use of one of the alternate methionine codons may also lead to generation of proteins capable of conferring resistance to plants against pathogenic filamentous fungi and/or Oomycetes. These proteins are encompassed within the scope of the present invention. It will be understood that, when expressed in plants, it will be necessary to alter the alternate start codon to ATG for proper translation. In addition, the translation initiator methionine of a polypeptide of the disclosure may be cleaved off post translationally. One skilled in the art understands that the N-terminal translation initiator methionine can be removed by methionine aminopeptidase in many cellular expression systems. 
     As is known to the skilled Artisan, the polynucleotide coding sequence can be modified to add a codon at the position following the methionine start codon to create a restriction enzyme site for recombinant cloning purposes and/or for expression purposes. 
     A “signal peptide” is an amino acid sequence which is translated in conjunction with a protein and directs the protein to the secretory system (Chrispeels, J. J., (1991) Ann. Rev. Plant Phys. Plant Mol. Biol. 42:21-53). A signal peptide may form part of the polypeptides of the invention or may be added as described hereinabove. In plants, the signal peptide may preferably direct the protein to the apoplast, the primary site of fungal colonization. When the antifungal proteins of the invention are of bacterial origin, the native fungal signal peptide should be replaced by a plant signal peptide, for example by the maize β-expansin 1 signal peptide (Fursova O et al., Ann Bot. 2012 110(1):47-56; Wu Y et al., Plant Physiol. 2001 126(1) 222-232). Bacterial-originated anti-fungal protein may also include lipid-anchor sequences, which should be modified and/or replaced for effective expression in plant cells. 
     According to certain embodiments of the present invention, a signal peptide required for expression in specific bacterium or plant species needs to be added or replace the native signal peptide. It is to be explicitly understood that polynucleotides and polypeptides optimized for expression in plant or bacterial cells by modification of their native N-terminus are encompassed within the scope of the present invention, although the global identity of the modified polypeptide to its parent peptide may be less than 70%. A polypeptide that was modified by removal of a native signal peptide thereof is considered herein as a “fragment polypeptide” or a “derived polypeptide”, which includes the amino acid sequence of the mature polypeptide, without the native signal peptide of either a curated or an isolated natural polypeptide. As used herein, the term “optimized polypeptide” refers to a polypeptide encoded by a polynucleotide modified for optimized expression in a desired organism. 
     According to additional aspect, the present invention provides an isolated or recombinant polynucleotide encoding a polypeptide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous, or identical to an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506 and 2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof. According to certain embodiments, the present invention provides an isolated polynucleotide encoding a polypeptide comprising the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508 and 2635, 2444-2634, 2636-4252. According to additional embodiments, the present invention provides an isolated polypeptide consisting of the amino acid sequence set forth in any one of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508 and 2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof. According to certain embodiments, the present invention provides an isolated or recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence set forth in any one of SEQ ID NOs:507-598. According to certain embodiments, the present invention provides an isolated or recombinant polynucleotide encoding a polypeptide consisting of the amino acid sequence set forth in any one of SEQ ID NOs:507-508. Each possibility represents a separate embodiment of the present invention. 
     The invention also encompasses fragments of the above described polynucleotides and polypeptides having mutations, such as deletions, insertions or substitutions of one or more amino acids, either naturally occurring or man induced, either randomly or in a targeted fashion. 
     Expression in plants of polypeptides isolated from bacteria according to some embodiments of the present invention may require modifying the polypeptide sequences for optimized expression. For example, signal peptides forming part of bacterial polypeptides should be typically removed, and optionally replaced with adequate signal peptides which will direct the localization of the polypeptide to a desired plant compartment. 
     According to certain embodiments, the bacterial polypeptide fragment comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:4647-4799. 
     According to additional aspect, the present invention provides an isolated polynucleotide, a fragment or a mutant thereof, the polynucleotide comprising a nucleic acids sequence at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polynucleotide having a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous, or identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, 81-301 and 783, 592-782, 784-2443. According to certain embodiments, the present invention provides an isolated polynucleotide, a fragment or a mutant thereof, the polynucleotide comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-305 and 783, 592-782, 784-2443. According to additional embodiments, the present invention provides an isolated polynucleotide consisting of the nucleic acid sequence set forth in any one of SEQ ID NOs:40, 80, 22-39, 41-79, 81-305 and 783, 592-782, 784-2443. 
     According to certain aspects, the present invention provides a recombinant polynucleotide optimized for polypeptide expression in a bacterial expression system, wherein the polypeptide is capable of inhibiting the growth of at least one pathogenic fungus and/or Oomycete. 
     According to certain exemplary embodiment the bacterium is  Escherichia coli . According to these embodiments, the polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:4468, 4450-4467, and 4469-4646. According to certain embodiments, the recombinant polynucleotide comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:4271, 4253-4270, and 4272-4449. 
     According to certain exemplary embodiments, the recombinant polynucleotide optimized for polypeptide expression in the bacterial expression system further comprises said bacterium signal peptide. 
     The isolated polynucleotides and polypeptides of the present invention and the fragment thereof are associated with conferring and/or increasing the resistance of a plant to at least one pathogenic fungus and/or Oomycete. 
     According to some embodiments of the invention, the exogenous polynucleotide is a non-coding RNA. 
     As used herein the phrase ‘non-coding RNA” refers to an RNA molecule which does not encode an amino acid sequence (a polypeptide). Examples of such non-coding RNA molecules include, but are not limited to, an antisense RNA, a pre-miRNA (precursor of a microRNA), or a precursor of a Piwi-interacting RNA (piRNA). 
     Thus, the invention encompasses nucleic acid sequences described hereinabove; fragments thereof, sequences hybridizable therewith, sequences homologous thereto, sequences encoding similar polypeptides with different codon usage, altered sequences characterized by mutations, such as deletion, insertion or substitution of one or more nucleotides, either naturally occurring or man induced, either randomly or in a targeted fashion. 
     According to additional aspect, the present invention provides a nucleic acid construct comprising the isolated polynucleotide of the invention, further comprising at least one regulatory element for directing transcription of the nucleic acid sequence in a host plant cell. 
     According to some embodiments of the invention, expressing the exogenous polynucleotide of the invention within a plant is affected by transforming one or more cells of the plant with the exogenous polynucleotide, followed by generating a mature plant from the transformed cells and cultivating the mature plant under conditions suitable for expressing the exogenous polynucleotide within the mature plant. 
     According to some embodiments of the invention, the transformation is affected by introducing to the plant cell a nucleic acid construct which includes the exogenous polynucleotide of some embodiments of the invention and at least one promoter for directing transcription of the exogenous polynucleotide in a host cell (a plant cell). Further details of suitable transformation approaches are provided hereinbelow. 
     As mentioned, the nucleic acid construct according to some embodiments of the invention comprises a promoter sequence and the isolated polynucleotide of some embodiments of the invention. 
     According to some embodiments of the invention, the isolated polynucleotide is operably linked to the promoter sequence. 
     A coding nucleic acid sequence is “operably linked” to a regulatory sequence (e.g., promoter) if the regulatory sequence is capable of exerting a regulatory effect on the coding sequence linked thereto. 
     As used herein, the term “promoter” refers to a region of DNA which lies upstream of the transcriptional initiation site of a gene to which RNA polymerase binds to initiate transcription of RNA. The promoter controls where (e.g., which portion of a plant) and/or when (e.g., at which stage or condition in the lifetime of an organism) the gene is expressed. 
     According to some embodiments of the invention, the promoter is heterologous to the isolated polynucleotide and/or to the host cell. 
     As used herein the phrase “heterologous promoter” refers to a promoter from a different species or from the same species but from a different gene locus as of the isolated polynucleotide sequence. 
     According to some embodiments of the invention, the isolated polynucleotide is heterologous to the plant cell (e.g., the polynucleotide is derived from a different plant species when compared to the plant cell, thus the isolated polynucleotide and the plant cell are not from the same plant species). 
     Any suitable promoter sequence can be used by the nucleic acid construct of the present invention. Preferably the promoter is selected from the group consisting of a constitutive promoter, a tissue-specific, or biotic-stress specific promoter, particularly promoters inducible by fungi infection. 
     According to some embodiments of the invention, the promoter is a plant promoter, which is suitable for expression of the exogenous polynucleotide in a plant cell. 
     Suitable promoters for expression in planta include, but are not limited to, Wheat SPA promoter (SEQ ID NO:1; Albani et al, 1997. Plant Cell, 9:171-184); wheat LMW [SEQ ID NO:2 (longer LMW promoter) and SEQ ID NO:3 (LMW promoter)]; HMW glutenin-1 [SEQ ID NO:4; (Wheat HMW glutenin-1 longer promoter); and SEQ ID NO:5 (Wheat HMW glutenin-1 Promoter); Thomas and Flavell, 1990. The Plant Cell 2:1171-1180; Furtado et al., 2009. Plant Biotechnology Journal 7:240-253]; wheat alpha, beta and gamma gliadins [e.g., SEQ ID NO:6 (wheat alpha gliadin, B genome, promoter); SEQ ID NO:7 (wheat gamma gliadin promoter); Rafalski J A et al. 1984. EMBO 3:1409-1415], wheat TdPR60 [SEQ ID NO:8 (wheat TdPR60 longer promoter) or SEQ ID NO:9 (wheat TdPR60 promoter); Kovalchuk et al., 2009. Plant Mol Biol 71:81-98], maize Ub1 Promoter [cultivar Nongda 105 (SEQ ID NO:10); GenBank: DQ141598.1; Taylor et al., 1993. Plant Cell Rep 12: 491-495; and cultivar B73 (SEQ ID NO:11; Christensen, A H et al. 1992. Plant Mol. Biol. 18(4):675-689); rice actin 1 (SEQ ID NO:12; Mc Elroy et al. 1990, The Plant Cell (2):163-171 rice GOS2 [SEQ ID NO:13 (rice GOS2 longer promoter) and SEQ ID NO:14 (rice GOS2 Promoter); De Pater et al. 1992. Plant J. 2: 837-44], arabidopsis Pho1 [SEQ ID NO:15 (arabidopsis Pho1 Promoter); Hamburger et al., Plant Cell. 2002; 14: 889-902], ExpansinB promoters, e.g., rice ExpB5 [SEQ ID NO:16 (rice ExpB5 longer promoter) and SEQ ID NO:17 (rice ExpB5 promoter)] and Barley ExpB1 [SEQ ID NO:18 (barley ExpB1 Promoter); Won et al. Mol Cells. 2010. 30:369-76], barley SS2 (sucrose synthase 2; SEQ ID NO:19; Guerin and Carbonero, 1997. Plant Physiology 114(1):55-62), and rice PG5a (SEQ ID NO:20; U.S. Pat. No. 7,700,835; Nakase et al., 1996. Plant Mol Biol. 32:621-30). 
     Suitable constitutive promoters include, for example, CaMV 35S promoter [SEQ ID NO:21 (CaMV 35S (pQXNc) Promoter); SEQ ID NO:22 (PJJ 35S from Brachypodium); SEQ ID NO:23 (CaMV 35S (OLD) Promoter; Odell et al., Nature 313:810-812, 1985)], Arabidopsis At6669 promoter [SEQ ID NO:24 (Arabidopsis At6669 (OLD) Promoter; see PCT Publication No. WO04081173 or the new At6669 promoter (SEQ ID NO:25 (Arabidopsis At6669 (NEW) Promoter)]; maize Ub1 Promoter [cultivar Nongda 105 (SEQ ID NO:10); and cultivar B73 (SEQ ID NO:11)]; rice actin 1 (SEQ ID NO:12); pEMU (Last et al., 1991. Theor. Appl. Genet. 81:581-588); CaMV 19S (Nilsson et al., 1997. Physiol. Plant 100:456-462); rice GOS2 [SEQ ID NO:13 (rice GOS2 longer Promoter) and SEQ ID NO: 14 (rice GOS2 Promoter); RBCS promoter (SEQ ID NO:26); Rice cyclophilin (Bucholz et al., 1994 Plant Mol Biol. 25(5):837-43); Maize H3 histone (Lepetit et al., 1992 Mol. Gen. Genet. 231: 276-285); Actin 2 (An et al., 1996. Plant J. 10(1); 107-121) and Synthetic Super MAS (Ni et al., 1995. The Plant Journal 7: 661-676). Other constitutive promoters include those in U.S. Pat. Nos. 5,659,026; 5,608,149; 5,608,144; 5,604,121; 5,569,597: 5,466,785; 5,399,680; 5,268,463; and 5,608,142. 
     Suitable tissue-specific promoters include, but are not limited to, leaf-specific promoters [e.g., AT5G06690 (Thioredoxin), high expression, SEQ ID NO:27); AT5G61520 (AtSTP3, low expression, SEQ ID NO:28, described in Buttner et al., 2000. Plant, Cell and Environment 23:175-184); or the promoters described in Yamamoto et al., 1997. Plant J. 12:255-265; Kwon et al., 1994. Plant Physiol. 105:357-67; Yamamoto et al., 1994. Plant Cell Physiol. 35:773-778; Gotor et al., 1993. Plant J. 3:509-18; Orozco et al., Plant Mol. Biol. 1993. 23:1129-1138; and Matsuoka et al., 1993. Proc. Natl. Acad. Sci. USA 90:9586-9590; as well as Arabidopsis STP3 (AT5G61520) promoter (Buttner et al., 2000. Plant, Cell and Environment 23:175-184]; seed-preferred promoters [e.g., Napin (originated from  Brassica napus  which is characterized by a seed specific promoter activity; Stuitje A. R. et. al. 2003. Plant Biotechnology Journal 1(4):301-309; SEQ ID NO:29 ( Brassica napus  NAPIN Promoter) from seed specific genes (Simon, et al., 1985. Plant Mol. Biol. 5:191; Scofield, et al., 1987. J. Biol. Chem. 262:12202; Baszczynski, et al., 1990. Plant Mol. Biol. 14:633), rice PG5a (SEQ ID NO:20; U.S. Pat. No. 7,700,835), early seed development  Arabidopsis  BAN (AT1G61720) (SEQ ID NO:30, US 2009/0031450), late seed development Arabidopsis ABI3 (AT3G24650) (SEQ ID NO:31 (Arabidopsis ABI3 (AT3G24650) longer Promoter) or SEQ ID NO:32 (Arabidopsis ABI3 (AT3G24650) Promoter)) (Ng et al., 2004. Plant Molecular Biology 54: 25-38), Brazil Nut albumin (Pearson&#39; et al., 1992. Plant Mol. Biol. 18: 235-245), legumin (Ellis, et al. 1988. Plant Mol. Biol. 10: 203-214), Glutelin (rice) (Takaiwa et al., 1986. Mol. Gen. Genet. 208:15-22; Takaiwa et al., 1987. FEBS Letts. 221: 43-47), Zein (Matzke et al., 1990. Plant Mol Biol, (143):323-332), napA (Stalberg et al., 1996. Planta 199:515-519); Wheat SPA (SEQ ID NO:1); sunflower oleosin (Cummins et al., 1992. Plant Mol. Biol. 19: 873-876); endosperm specific promoters [e.g., wheat LMW (SEQ ID NO:2; Wheat LMW Longer Promoter), and SEQ ID NO:3 (Wheat LMW Promoter)] and HMW glutenin-1 [(SEQ ID NO:4 (Wheat HMW glutenin-1 longer Promoter); and SEQ ID NO:5 (Wheat HMW glutenin-1 Promoter); Colot et al., Mol Gen Genet 216:81-90, 1989; Olin et al., NAR 17:461-2, 1989), wheat alpha, beta and gamma gliadins (SEQ ID NO:6 (wheat alpha gliadin (B genome) promoter); SEQ ID NO:7 (wheat gamma gliadin promoter); Barley ltrl promoter, barley B1, C, D hordein (Cho et al., Theor Appl Gen 98:1253-62, 1999; Muller et al., Plant J 4:343-55, 1993; Sorenson et al., Mol Gen Genet 250:750-60, 1996), Barley DOF (Mena et al., 1998. The Plant Journal 116(1):53-62), Biz2 (EP99106056.7), Barley SS2 (SEQ ID NO:19), wheat Tarp60 (Kovalchuk et al., 2009. Plant Mol Biol 71:81-98), barley D-hordein (D-Hor) and B-hordein (B-Hor) (Agnelo F et al., 2009. Plant Biotech J 793):240-253)], Synthetic promoter (Vicente-Carbajosa et al., 1998. Plant J. 13: 629-640), rice prolamin NRP33, rice-globulin Glb-1 (Wu et al., 1998. Plant Cell Physiology 39(8) 885-889), rice alpha-globulin REB/OHP-1 (Nakase et al. 1997. Plant Mol. Biol. 33: 513-S22), rice ADP-glucose PP (Russell et al., Trans Res 6:157-68, 1997), maize ESR gene family (Opsahl-Ferstad et al., Plant J 12:235-46, 1997), sorgum gamma-kafirin (DeRose et al., PMB 32:1029-35, 1996)], embryo specific promoters [e.g., rice OSH1 (Sato et al., Proc. Natl. Acad. Sci. USA, 93: 8117-8122, 1996), KNOX (Postma-Haarsma et al., 1999. Plant Mol. Biol. 39:257-71), rice oleosin (Wu et al., 1998. J. Biochem., 123:386], and flower-specific promoters [e.g., AtPRP4, chalene synthase (chsA) (Van der Meer et al., 1990. Plant Mol. Biol. 15, 95-109), LAT52 (Twell et al., 1989. Mol. Gen Genet 217:240-245),  Arabidopsis apetala -3 (Tilly et al., 1998. Development 125:1647-57),  Arabidopsis apetala  1 (AT1G69120, AP1) (SEQ ID NO:33 (Arabidopsis (AT1G69120) APETALA 1)) (Hempel et al., 1997. Development 124:3845-3853)], and root promoters [e.g., the ROOTP promoter [SEQ ID NO:34]; rice ExpB5 [SEQ ID NO:17 (rice ExpB5 Promoter); or SEQ ID NO:16 (rice ExpB5 longer Promoter)] and barley ExpB1 promoters (SEQ ID NO:18) (Won et al. 2010. Mol. Cells 30: 369-376); Arabidopsis ATTPS-CIN (AT3G25820) promoter (SEQ ID NO:35; Chen et al., 2004. Plant Phys 135:1956-66); Arabidopsis Pho1 promoter (SEQ ID NO: 15), which is also slightly induced by stress]. 
     The nucleic acid construct of some embodiments of the invention can further include an appropriate selectable marker and/or an origin of replication. According to some embodiments of the invention, the nucleic acid construct utilized is a shuttle vector, which can propagate both in  E. coli  (wherein the construct comprises an appropriate selectable marker and origin of replication) and be compatible with propagation in cells. The construct according to the present invention can be, for example, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus or an artificial chromosome. 
     The nucleic acid construct of some embodiments of the invention can be utilized to stably or transiently transform plant cells. In stable transformation, the exogenous polynucleotide is integrated into the plant genome and as such it represents a stable and inherited trait. In transient transformation, the exogenous polynucleotide is expressed by the cell transformed but it is not integrated into the genome and as such it represents a transient trait. 
     There are various methods of introducing foreign genes into both monocotyledonous and dicotyledonous plants (Potrykus, I., Annu. Rev. Plant. Physiol. &amp; Plant. Mol. Biol. 1991. 42:205-225; Shimamoto et al., 1989. Nature 338:274-276). 
     The principle methods of causing stable integration of exogenous DNA into plant genomic DNA include two main approaches: 
     (i)  Agrobacterium -mediated gene transfer: Klee et al., (1987) Annu. Rev. Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes, eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds. Kung, S. and Arntzen, C. J., Butterworth Publishers, Boston, Mass. (1989) p. 93-112. 
     (ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 52-68; including methods for direct uptake of DNA into protoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNA uptake induced by brief electric shock of plant cells: Zhang et al. Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986) 319:791-793. DNA injection into plant cells or tissues by particle bombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al. Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990) 79:206-209; by the use of micropipette systems: Neuhaus et al., Theor. Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant. (1990) 79:213-217; glass fibers or silicon carbide whisker transformation of cell cultures, embryos or callus tissue, U.S. Pat. No. 5,464,765 or by the direct incubation of DNA with germinating pollen, DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman, G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p. 197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719. 
     The  Agrobacterium  system includes the use of plasmid vectors that contain defined DNA segments that integrate into the plant genomic DNA. Methods of inoculation of the plant tissue vary depending upon the plant species and the Agrobacterium delivery system. A widely used approach is the leaf disc procedure which can be performed with any tissue explant that provides a good source for initiation of whole plant differentiation. See, e.g., Horsch et al. in Plant Molecular Biology Manual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. A supplementary approach employs the Agrobacterium delivery system in combination with vacuum infiltration. The Agrobacterium system is especially viable in the creation of transgenic dicotyledonous plants. 
     There are various methods of direct DNA transfer into plant cells. In electroporation, the protoplasts are briefly exposed to a strong electric field. In microinjection, the DNA is mechanically injected directly into the cells using very small micropipettes. In microparticle bombardment, the DNA is adsorbed on microprojectiles such as magnesium sulfate crystals or tungsten particles, and the microprojectiles are physically accelerated into cells or plant tissues. 
     Following stable transformation plant propagation is exercised. The most common method of plant propagation is by seed. Regeneration by seed propagation, however, has the deficiency that due to heterozygosity there is a lack of uniformity in the crop, since seeds are produced by plants according to the genetic variances governed by Mendelian rules. Basically, each seed is genetically different and each will grow with its own specific traits. Therefore, it is preferred that the transformed plant be produced such that the regenerated plant has the identical traits and characteristics of the parent transgenic plant. Therefore, it is preferred that the transformed plant be regenerated by micropropagation which provides a rapid, consistent reproduction of the transformed plants. 
     Micropropagation is a process of growing new generation plants from a single piece of tissue that has been excised from a selected parent plant or cultivar. This process permits the mass reproduction of plants having the preferred tissue expressing the fusion protein. The new generation plants which are produced are genetically identical to, and have all of the characteristics of, the original plant. Micropropagation allows mass production of quality plant material in a short period of time and offers a rapid multiplication of selected cultivars in the preservation of the characteristics of the original transgenic or transformed plant. The advantages of cloning plants are the speed of plant multiplication and the quality and uniformity of plants produced. 
     Micropropagation is a multi-stage procedure that requires alteration of culture medium or growth conditions between stages. Thus, the micropropagation process involves four basic stages: Stage one, initial tissue culturing; stage two, tissue culture multiplication; stage three, differentiation and plant formation; and stage four, greenhouse culturing and hardening. During stage one, initial tissue culturing, the tissue culture is established and certified contaminant-free. During stage two, the initial tissue culture is multiplied until a sufficient number of tissue samples are produced from the seedlings to meet production goals. During stage three, the tissue samples grown in stage two are divided and grown into individual plantlets. At stage four, the transformed plantlets are transferred to a greenhouse for hardening where the plants&#39; tolerance to light is gradually increased so that it can be grown in the natural environment. 
     According to some embodiments of the invention, the transgenic plant is generated by transient transformation of leaf cells, meristematic cells or the whole plant. 
     Transient transformation can be performed by any of the direct DNA transfer methods described above or by viral infection using modified plant viruses. 
     Viruses that have been shown to be useful for the transformation of plant hosts include, for example, Cauliflower mosaic virus (CaMV), Tobacco mosaic virus (TMV), brome mosaic virus (BMV) and Bean Common Mosaic Virus (BV or BCMV). Methods for transformation of plants using plant viruses are well known in the art; see, e.g. U.S. Pat. No. 4,855,237; Gluzman, Y. et al., Communications in Molecular Biology: Viral Vectors, Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988); and Mortimer C et al., 2015. Current Opinion in Biotechnology 32:85-92). Pseudovirus particles for use in expressing foreign DNA in many hosts, including plants are described in WO 87/06261. 
     According to some embodiments of the invention, the virus used for transient transformations is avirulent and thus is incapable of causing severe symptoms such as reduced growth rate, mosaic, ring spots, leaf roll, yellowing, streaking, pox formation, tumor formation and pitting. A suitable avirulent virus may be a naturally occurring avirulent virus or an artificially attenuated virus. Virus attenuation may be performed by using methods well known in the art including, but not limited to, sub-lethal heating, chemical treatment or by directed mutagenesis techniques such as described, for example, by Kurihara and Watanabe (Molecular Plant Pathology 4:259-269, 2003), Gal-On et al., J Gener Viriol 73: 2183-87 (1992), Atreya et al. Viriology 191:106-11 (1992) and Huet et al. Viriology 75: 1407-14 (1994). 
     Suitable virus strains can be obtained from available sources such as, for example, the American Type culture Collection (ATCC) or by isolation from infected plants. Isolation of viruses from infected plant tissues can be effected by techniques well known in the art such as described, for example by Foster and Taylor, Eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology Vol 81 Humana Press, 1998). Briefly, tissues of an infected plant believed to contain a high concentration of a suitable virus, preferably young leaves and flower petals, are ground in a buffer solution (e.g., phosphate buffer solution) to produce a virus infected sap which can be used in subsequent inoculations. 
     Construction of plant RNA viruses for the introduction and expression of non-viral exogenous polynucleotide sequences in plants is demonstrated by the above references as well as by Dawson, W. O. et al., 1989. Virology 172:285-292; Takamatsu et al. 1987. EMBO J 6:307-311; French et al. 1986. Science 231:1294-1297; Takamatsu et al. 1990. FEBS Letters 269:73-76; and U.S. Pat. No. 5,316,931. 
     When the virus is a DNA virus, suitable modifications can be made to the virus itself. Alternatively, the virus can first be cloned into a bacterial plasmid for ease of constructing the desired viral vector with the foreign DNA. The virus can then be excised from the plasmid. If the virus is a DNA virus, a bacterial origin of replication can be attached to the viral DNA, which is then replicated by the bacteria. Transcription and translation of this DNA will produce the coat protein which will encapsidate the viral DNA. If the virus is an RNA virus, the virus is generally cloned as a cDNA and inserted into a plasmid. The plasmid is then used to make all of the constructions. The RNA virus is then produced by transcribing the viral sequence of the plasmid and translation of the viral genes to produce the coat protein(s) which encapsidate the viral RNA. 
     In one embodiment, a plant viral polynucleotide is provided in which the native coat protein coding sequence has been deleted from a viral polynucleotide, a non-native plant viral coat protein coding sequence and a non-native promoter, preferably the subgenomic promoter of the non-native coat protein coding sequence, capable of expression in the plant host, packaging of the recombinant plant viral polynucleotide, and ensuring a systemic infection of the host by the recombinant plant viral polynucleotide, has been inserted. Alternatively, the coat protein gene may be inactivated by insertion of the non-native polynucleotide sequence within it, such that a protein is produced. The recombinant plant viral polynucleotide may contain one or more additional non-native subgenomic promoters. Each non-native subgenomic promoter is capable of transcribing or expressing adjacent genes or polynucleotide sequences in the plant host and incapable of recombination with each other and with native subgenomic promoters. Non-native (foreign) polynucleotide sequences may be inserted adjacent the native plant viral subgenomic promoter or the native and a non-native plant viral subgenomic promoters if more than one polynucleotide sequence is included. The non-native polynucleotide sequences are transcribed or expressed in the host plant under control of the subgenomic promoter to produce the desired products. 
     In a second embodiment, a recombinant plant viral polynucleotide is provided as in the first embodiment except that the native coat protein coding sequence is placed adjacent one of the non-native coat protein subgenomic promoters instead of a non-native coat protein coding sequence. 
     In a third embodiment, a recombinant plant viral polynucleotide is provided in which the native coat protein gene is adjacent its subgenomic promoter and one or more non-native subgenomic promoters have been inserted into the viral polynucleotide. The inserted non-native subgenomic promoters are capable of transcribing or expressing adjacent genes in a plant host and are incapable of recombination with each other and with native subgenomic promoters. Non-native polynucleotide sequences may be inserted adjacent the non-native subgenomic plant viral promoters such that the sequences are transcribed or expressed in the host plant under control of the subgenomic promoters to produce the desired product. 
     In a fourth embodiment, a recombinant plant viral polynucleotide is provided as in the third embodiment except that the native coat protein coding sequence is replaced by a non-native coat protein coding sequence. 
     The viral vectors are encapsidated by the coat proteins encoded by the recombinant plant viral polynucleotide to produce a recombinant plant virus. The recombinant plant viral polynucleotide or recombinant plant virus is used to infect appropriate host plants. The recombinant plant viral polynucleotide is capable of replication in the host, systemic spread in the host, and transcription or expression of foreign gene(s) (exogenous polynucleotide) in the host to produce the desired protein. 
     Techniques for inoculation of viruses to plants may be found, for example, in Foster and Taylor, Eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol 81)”, Humana Press, 1998; Maramorosh and Koprowski, Eds. “Methods in Virology” 7 vols, Academic Press, New York 1967-1984; Hill, S. A. “Methods in Plant Virology”, Blackwell, Oxford, 1984; Walkey, D. G. A. “Applied Plant Virology”, Wiley, New York, 1985; and Kado and Agrawa, eds. “Principles and Techniques in Plant Virology”, Van Nostrand-Reinhold, New York. 
     In addition to the above, the polynucleotide of the present invention can also be introduced into a chloroplast genome thereby enabling chloroplast expression. 
     A technique for introducing exogenous polynucleotide sequences to the genome of the chloroplasts is known. This technique involves the following procedures. First, plant cells are chemically treated so as to reduce the number of chloroplasts per cell to about one. Then, the exogenous polynucleotide is introduced via particle bombardment into the cells with the aim of introducing at least one exogenous polynucleotide molecule into the chloroplasts. The exogenous polynucleotides selected such that it is integratable into the chloroplast&#39;s genome via homologous recombination which is readily performed by enzymes inherent to the chloroplast. To this end, the exogenous polynucleotide includes, in addition to a gene of interest, at least one polynucleotide stretch which is derived from the chloroplast&#39;s genome. In addition, the exogenous polynucleotide includes a selectable marker, which serves by sequential selection procedures to ascertain that all or substantially all of the copies of the chloroplast genomes following such selection will include the exogenous polynucleotide. Further details relating to this technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507 which are incorporated herein by reference. A polypeptide can thus be produced by the protein expression system of the chloroplast and become integrated into the chloroplast&#39;s inner membrane. 
     According to some embodiments of the invention, the transformed plant is homozygote to the transgene (i.e., the exogenous polynucleotide of some embodiments of the invention), and accordingly all seeds generated thereby include the transgene. 
     Expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing multiple nucleic acid constructs, each including a different exogenous polynucleotide, into a single plant cell. The transformed cell can then be regenerated into a mature plant using the methods described hereinabove. 
     Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing into a single plant-cell a single nucleic-acid construct including a plurality of different exogenous polynucleotides. Such a construct can be designed with a single promoter sequence which can transcribe a polycistronic messenger RNA including all the different exogenous polynucleotide sequences. To enable co-translation of the different polypeptides encoded by the polycistronic messenger RNA, the polynucleotide sequences can be inter-linked via an internal ribosome entry site (IRES) sequence which facilitates translation of polynucleotide sequences positioned downstream of the IRES sequence. In this case, a transcribed polycistronic RNA molecule encoding the different polypeptides described above will be translated from both the capped 5′ end and the two internal IRES sequences of the polycistronic RNA molecule to thereby produce in the cell all different polypeptides. Alternatively, the construct can include several promoter sequences each linked to a different exogenous polynucleotide sequence. 
     The plant cell transformed with the construct including a plurality of different exogenous polynucleotides can be regenerated into a mature plant, using the methods described hereinabove. 
     Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by introducing different nucleic acid constructs, including different exogenous polynucleotides, into a plurality of plants. The regenerated transformed plants can then be cross-bred and resultant progeny selected for superior abiotic stress tolerance, water use efficiency, fertilizer use efficiency, early flowering, grain filling period, harvest index, plant height, growth, biomass, yield and/or vigor traits, using conventional plant breeding techniques. 
     According to certain embodiments, enhancing the resistance of the plant or part thereof to the pathogenic fungus comprises reducing the expression and/or activity of the at least one polypeptide compared to its expression and/or activity in the control plant. 
     Any method as is known in the art for reducing the expression and/or activity of a plant endogenous protein and the polynucleotide encoding same can be used according to the teachings of the resent invention. 
     According to certain embodiment of the invention, reducing the expression and/or activity of a polypeptide of the invention within a plant cell comprising transforming the plant cell with a polynucleotide that inhibits the expression of said polypeptide. The polynucleotide may inhibit the transcription or translation of a polynucleotide encoding said polypeptide or can encode for an inhibitory polypeptide interfering with the translation or activity of said polypeptide. 
     Polynucleotide-Based Methods 
     According to some embodiments of the present disclosure, a plant is transformed with a polynucleotide that inhibits the expression of a polypeptide of the invention. According to certain exemplary embodiments, the plant cell is transformed within a construct capable of expressing the inhibitory polynucleotide. The term “expression” as used herein refers to the biosynthesis of a gene product, including the transcription and/or translation of said gene product. For example, for the purposes of the present invention, a construct capable of expressing the inhibitory polynucleotide is capable of producing an RNA molecule that inhibits the transcription and/or translation of a polypeptide of the invention. The “expression” or “production” of a protein or polypeptide from a DNA molecule refers to the transcription and translation of the coding sequence to produce the protein or polypeptide, while the “expression” or “production” of a protein or polypeptide from an RNA molecule refers to the translation of the RNA coding sequence to produce the protein or polypeptide. Examples of polynucleotides that inhibit the expression of a CCT polypeptide are given below. 
     Sense Suppression/Co-Suppression 
     According to certain embodiments, inhibition of the expression of a polypeptide of the invention may be obtained by sense suppression or co-suppression. For co-suppression, a construct is designed to express an RNA molecule corresponding to all or part of a messenger RNA encoding the polypeptide in the “sense” orientation. Over-expression of the RNA molecule can result in reduced expression of the native gene. Accordingly, multiple plant lines transformed with the co-suppression constructs are screened to identify those that show the greatest inhibition of the polypeptide expression. 
     The polynucleotide used for co-suppression may correspond to all or part of the sequence encoding the polypeptide of the invention, all or part of the 5′ and/or 3′ untranslated region of said polypeptide transcript or all or part of both the coding sequence and the untranslated regions of a transcript encoding said polypeptide. In some embodiments where the polynucleotide comprises all or part of the coding region for said polypeptide, the construct is designed to eliminate the start codon of the polynucleotide so that no protein product will be translated. 
     Co-suppression may be used to inhibit the expression of plant genes to produce plants having undetectable protein levels for the proteins encoded by these genes. See, for example, Broin, et al., (2002) Plant Cell 14:1417-1432. Co-suppression may also be used to inhibit the expression of multiple proteins in the same plant. See, for example, U.S. Pat. No. 5,942,657. Methods for using co-suppression to inhibit the expression of endogenous genes in plants are described, for example, in Yu, et al., Phytochemistry (2003) 63:753-763 and U.S. Pat. Nos. 5,034,323, 5,283,184 and 5,942,657. The efficiency of co-suppression may be increased by including a poly-dT region in the expression cassette at a position 3′ to the sense sequence and 5′ of the polyadenylation signal. See for example, US Patent Application Publication Number 2002/0048814. Typically, such a nucleotide sequence has substantial sequence identity to the sequence of the transcript of the endogenous gene, optimally greater than about 65% sequence identity, more optimally greater than about 85% sequence identity, most optimally greater than about 95% sequence identity (U.S. Pat. Nos. 5,283,184 and 5,034,323). 
     Antisense Suppression 
     According to some embodiments, inhibition of the expression of a polypeptide of the invention may be obtained by antisense suppression. For antisense suppression, the construct is designed to express an RNA molecule complementary to all or part of a messenger RNA encoding the polypeptide. Over-expression of the antisense RNA molecule can result in reduced expression of the native gene. Accordingly, multiple plant lines transformed with the antisense RNA are screened to identify those that show the greatest inhibition of said polypeptide expression. 
     The polynucleotide for use in antisense suppression may correspond to all or part of the complement of the sequence encoding the polypeptide of the invention, all or part of the complement of the 5′ and/or 3′ untranslated region of its transcript or all or part of the complement of both the coding sequence and the untranslated regions of a transcript encoding said polypeptide. In addition, the antisense polynucleotide may be fully complementary (i.e., 100% identical to the complement of the target sequence) or partially complementary (i.e., less than 100% identical to the complement of the target sequence) to the target sequence. Antisense suppression may be used to inhibit the expression of multiple proteins in the same plant. See, for example, U.S. Pat. No. 5,942,657. Furthermore, portions of the antisense nucleotides may be used to disrupt the expression of the target gene. Generally, sequences of at least 50 nucleotides, 100 nucleotides, 200 nucleotides, 300, 400, 450, 500, 550 or greater may be used. Methods for using antisense suppression to inhibit the expression of endogenous genes in plants are described, for example, in Liu, et al., (2002) Plant Physiol. 129:1732-1743 and U.S. Pat. Nos. 5,759,829 and 5,942,657. Efficiency of antisense suppression may be increased by including a poly-dT region in the expression cassette at a position 3′ to the antisense sequence and 5′ of the polyadenylation signal (see, e.g. US Patent Application Publication Number 2002/0048814). 
     Double-Stranded RNA Interference 
     According to some embodiments, inhibition of the expression of a polypeptide of the invention may be obtained by double-stranded RNA (dsRNA) interference. For dsRNA interference, a sense RNA molecule like that described above for co-suppression and an antisense RNA molecule that is fully or partially complementary to the sense RNA molecule are expressed in the same cell, resulting in inhibition of the expression of the corresponding endogenous messenger RNA. 
     Expression of the sense and antisense molecules can be accomplished by designing the DNA construct to comprise both a sense sequence and an antisense sequence. Alternatively, separate constructs may be used for the sense and antisense sequences. Multiple plant lines transformed with the dsRNA interference construct(s) are then screened to identify plant lines that show the greatest inhibition of the expression of the polypeptide. Methods for using dsRNA interference to inhibit the expression of endogenous plant genes are described in Waterhouse, et al., (1998) Proc. Natl. Acad. Sci. USA 95:13959-13964, Liu, et al., (2002) Plant Physiol. 129:1732-1743 and WO 1999/49029, WO 1999/53050, WO 1999/61631 and WO 2000/49035. 
     Hairpin RNA Interference and Intron-Containing Hairpin RNA Interference 
     According to some embodiments, inhibition of the expression of a polypeptide of the invention may be obtained by hairpin RNA (hpRNA) interference or intron-containing hairpin RNA (ihpRNA) interference. These methods are highly efficient at inhibiting the expression of endogenous genes. See, Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 4:29-38 and the references cited therein. 
     For hpRNA interference, the construct is designed to express an RNA molecule that hybridizes with itself to form a hairpin structure that comprises a single-stranded loop region and a base-paired stem. The base-paired stem region comprises a sense sequence corresponding to all or part of the endogenous messenger RNA encoding the gene whose expression is to be inhibited and an antisense sequence that is fully or partially complementary to the sense sequence. Thus, the base-paired stem region of the molecule generally determines the specificity of the RNA interference. hpRNA molecules are highly efficient at inhibiting the expression of endogenous genes, and the RNA interference they induce is inherited by subsequent generations of plants. See, for example, Chuang and Meyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:4985-4990; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731 and Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 4:29-38. Methods for using hpRNA interference to inhibit or silence the expression of genes are described, for example, in US Patent Application Publication Number 2003/0175965. A transient assay for the efficiency of hpRNA constructs to silence gene expression in vivo has been described by Panstruga, et al., (2003) Mol. Biol. Rep. 30:135-140. 
     For Intron-Containing Hairpin RNA (ihpRNA) interference, the interfering molecules have the same general structure as for hpRNA, but the RNA molecule additionally comprises an intron that is capable of being spliced in the cell in which the ihpRNA is expressed. The use of an intron minimizes the size of the loop in the hairpin RNA molecule following splicing, and this increases the efficiency of interference. See, for example, Smith, et al., (2000) Nature 407:319-320. In fact, Smith, et al., shows 100% suppression of endogenous gene expression using ihpRNA-mediated interference. Methods for using ihpRNA interference to inhibit the expression of endogenous plant genes are described, for example, in US Patent Application Publication Number 2003/0180945. 
     The expression cassette for hpRNA interference may also be designed such that the sense sequence and the antisense sequence do not correspond to an endogenous RNA. In this embodiment, the sense and antisense sequence flank a loop sequence that comprises a nucleotide sequence corresponding to all or part of the endogenous messenger RNA of the target gene. Thus, it is the loop region that determines the specificity of the RNA interference. See, for example, WO 2002/00904. 
     Amplicon-Mediated Interference 
     Amplicon expression cassettes comprise a plant virus-derived sequence that contains all or part of the target gene but generally not all of the genes of the native virus. The viral sequences present in the transcription product of the construct allow the transcription product to direct its own replication. The transcripts produced by the amplicon may be either sense or antisense relative to the target sequence (i.e., the messenger RNA for the polypeptide of the invention). Methods of using amplicons to inhibit the expression of endogenous plant genes are described, for example, in Angell and Baulcombe, (1997) EMBO J. 16:3675-3684, Angell and Baulcombe, (1999) Plant J. 20:357-362. 
     Ribozymes 
     According to some embodiments, the polynucleotide expressed by the construct of the invention is catalytic RNA or has ribozyme activity specific for the messenger RNA of a polypeptide of the invention. Thus, the polynucleotide causes the degradation of the endogenous messenger RNA, resulting in reduced expression of said polypeptide. This method is described, for example, in U.S. Pat. No. 4,987,071. 
     Small Interfering RNA or Micro RNA 
     According to certain embodiments of the invention, inhibition of the expression of a polypeptide of the invention may be obtained by RNA interference by expression of a gene encoding a micro RNA (miRNA). miRNAs are regulatory agents consisting of about 22 ribonucleotides. miRNAs are highly efficient at inhibiting the expression of endogenous genes. See, for example, Palatnikl J F et al., (2003) Nature 425:257-263. 
     For miRNA interference, the construct is designed to express an RNA molecule that is modeled on an endogenous miRNA gene. The miRNA gene encodes an RNA that forms a hairpin structure containing a 22-nucleotide sequence that is complementary to another endogenous gene (target sequence). For suppressing the expression of a polypeptide of the invention, the 22-nucleotide sequence is selected from the polypeptide transcript sequence and contains 22 nucleotides of said transcript sequence in sense orientation and 21 nucleotides of a corresponding antisense sequence that is complementary to the sense sequence. miRNA molecules are highly efficient at inhibiting the expression of endogenous genes and the RNA interference they induce is inherited by subsequent generations of plants. 
     Polypeptide-Based Inhibition of Gene Expression 
     According to certain additional or alternative embodiments, the inhibitory polynucleotide encodes a zinc finger protein that binds to a gene encoding a polypeptide of the invention, resulting in reduced expression of the gene. In particular embodiments, the zinc finger protein binds to a regulatory region of a polypeptide encoding gene. In other embodiments, the zinc finger protein binds to a messenger RNA encoding said polypeptide and prevents its translation. Methods of selecting sites for targeting by zinc finger proteins have been described, for example, in U.S. Pat. No. 6,453,242 and methods for using zinc finger proteins to inhibit the expression of genes in plants are described, for example, in U.S. Pat. No. 7,151,201. 
     Polypeptide-Based Inhibition of Protein Activity 
     According to certain additional or alternative embodiments, the polynucleotide encodes an antibody that binds to a polypeptide of the invention and reduces the activity of the polypeptide. In another embodiment, the binding of the antibody results in increased turnover of the antibody-polypeptide complex by cellular quality control mechanisms. The expression of antibodies in plant cells and the inhibition of molecular pathways by expression and binding of antibodies to proteins in plant cells are well known in the art. See, for example, Conrad and Sonnewald, (2003) Nature Biotech. 21:35-36. 
     According to some embodiments of the invention, up-regulation or down regulation of the expression and/or activity of the polypeptide of the invention is achieved by means of genome editing. 
     Genome editing is a reverse genetics method which uses artificially engineered nucleases to cut and create specific double-stranded breaks at a desired location(s) in the genome, which are then repaired by cellular endogenous processes such as, homology directed repair (HDR) and non-homologous end-joining (NHEJ). NHEJ directly joins the DNA ends in a double-stranded break, while HDR utilizes a homologous sequence as a template for regenerating the missing DNA sequence at the break point. In order to introduce specific nucleotide modifications to the genomic DNA, a DNA repair template containing the desired sequence must be present during HDR. Genome editing cannot be performed using traditional restriction endonucleases since most restriction enzymes recognize a few base pairs on the DNA as their target and the probability is very high that the recognized base pair combination will be found in many locations across the genome resulting in multiple cuts not limited to a desired location. To overcome this challenge and create site-specific single- or double-stranded breaks, several distinct classes of nucleases have been discovered and bioengineered to date. These include the meganucleases, Zinc finger nucleases (ZFNs), transcription-activator like effector nucleases (TALENs) and CRISPR/Cas system. 
     Genome editing is a powerful tool to impact target traits by modifications of the target plant genome sequence. Such modifications can result in new or modified alleles or regulatory elements. 
     In addition, the traces of genome-edited techniques can be used for marker assisted selection (MAS) as is further described hereinunder. Target plants for the mutagenesis/genome editing methods according to the invention are any plants of interest including monocot or dicot plants. 
     Over-expression of a polypeptide by genome editing can be achieved by: (i) replacing an endogenous sequence encoding the polypeptide of interest or a regulatory sequence under which it is placed, and/or (ii) inserting a new gene encoding the polypeptide of interest in a targeted region of the genome, and/or (iii) introducing point mutations which result in up-regulation of the gene encoding the polypeptide of interest (e.g., by altering the regulatory sequences such as promoter, enhancers, 5′-UTR and/or 3′-UTR, or mutations in the coding sequence). 
     Down regulation of the expression of a polypeptide by gnome editing can be achieved by (i) replacing an endogenous sequence encoding a polypeptide negatively affecting a desired plant trait, according to some embodiments of the invention enhancing susceptibility of the plant to pathogenic fungi and/or Oomycete or replacing a regulatory sequence under which the endogenous sequence encoding the polypeptide is placed, and/or (ii) introducing point mutations which result in down-regulation of the gene encoding the polypeptide of interest (e.g., by altering the regulatory sequences such as promoter, enhancers, 5′-UTR and/or 3′-UTR, or mutations in the coding sequence). 
     Genome Editing Systems Overview 
     Several systems have been reported to enable genome editing implementation. Examples detailed herein below: 
     Meganucleases—Meganucleases are commonly grouped into four families: the LAGLIDADG family, the GIY-YIG family, the His-Cys box family and the HNH family. These families are characterized by structural motifs, which affect catalytic activity and recognition sequence. For instance, members of the LAGLIDADG family are characterized by having either one or two copies of the conserved LAGLIDADG motif. The four families of meganucleases are widely separated from one another with respect to conserved structural elements and, consequently, DNA recognition sequence specificity and catalytic activity. Meganucleases are found commonly in microbial species and have the unique property of having very long recognition sequences (&gt;14 bp) thus making them naturally very specific for cutting at a desired location. This can be exploited to make site-specific double-stranded breaks directing modifications in regulatory elements or coding regions upon introduction of the desired sequence. One of skill in the art can use these naturally occurring meganucleases, however the number of such naturally occurring meganucleases is limited. To overcome this challenge, mutagenesis and high throughput screening methods have been used to create meganuclease variants that recognize unique sequences. For example, various meganucleases have been fused to create hybrid enzymes that recognize a new sequence. Alternatively, DNA interacting amino acids of the meganuclease can be altered to design sequence specific meganucleases (see e.g., U.S. Pat. No. 8,021,867). Meganucleases can be designed using the methods described in e.g., Certo, M T et al., 2012. Nature Methods 9:073-975; U.S. Pat. Nos. 8,304,222; 8,021,867; 8,119,381; 8,124,369; 8,129,134; 8,133,697; 8,143,015; 8,143,016; 8,148,098; or 8,163,514. Alternatively, meganucleases with site specific cutting characteristics can be obtained using commercially available technologies e.g., Precision Biosciences&#39; Directed Nuclease Editor™ genome editing technology. 
     ZFNs and TALENs—Two distinct classes of engineered nucleases, zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), have both proven to be effective at producing targeted double-stranded breaks (Christian et al., 2010. Genetics 186:757-761; Kim et al., 1996. Proc. Natl. Acad. Sci. 93:1156-1160; Li et al., 2011. Nucleic Acids Res 39:359-372; Mahfouz et al., 2011. Proc. Natl. Acad. Sci; 108:2623-2628; Miller et al., 2010. Nat Biotechnol. 29:143-148). 
     Basically, ZFNs and TALENs restriction endonuclease technology utilizes a non-specific DNA cutting enzyme which is linked to a specific DNA binding domain (either a series of zinc finger domains or TALE repeats, respectively). Typically, a restriction enzyme whose DNA recognition site and cleaving site are separate from each other is selected. The cleaving portion is separated and then linked to a DNA binding domain, thereby yielding an endonuclease with very high specificity for a desired sequence. An exemplary restriction enzyme with such properties is Fokl. Additionally, Fokl has the advantage of requiring dimerization to have nuclease activity and this means the specificity increases dramatically as each nuclease partner recognizes a unique DNA sequence. To enhance this effect, Fokl nucleases have been engineered that can only function as heterodimers and have increased catalytic activity. The heterodimer functioning nucleases avoid the possibility of unwanted homodimer activity and thus increase specificity of the double-stranded break. 
     Thus, for example to target a specific site, ZFNs and TALENs are constructed as nuclease pairs, with each member of the pair designed to bind adjacent sequences at the targeted site. Upon transient expression in cells, the nucleases bind to their target sites and the Fokl domains heterodimerize to create a double-stranded break. Repair of these double-stranded breaks through the nonhomologous end-joining (NHEJ) pathway most often results in small deletions or small sequence insertions. Since each repair made by NHEJ is unique, the use of a single nuclease pair can produce an allelic series with a range of different deletions at the target site. The deletions typically range anywhere from a few base pairs to a few hundred base pairs in length, but larger deletions have successfully been generated in cell culture by using two pairs of nucleases simultaneously (Carlson et al., 2012. Proc. Natl. Acad. Sci 109:17382-17387; Lee et al., 2010. Genome Res 20:81-89). In addition, when a fragment of DNA with homology to the targeted region is introduced in conjunction with the nuclease pair, the double-stranded break can be repaired via homology directed repair to generate specific modifications (Li et al., 2011, ibid; Miller et al., 2010, ibid; Urnov et al., 2005. Nature, 435:646-651). 
     Although the nuclease portions of both ZFNs and TALENs have similar properties, the difference between these engineered nucleases is in their DNA recognition peptide. ZFNs rely on Cys2-His2 zinc fingers and TALENs on TALEs. Both of these DNA recognizing peptide domains have the characteristic that they are naturally found in combinations in their proteins. Cys2-His2 Zinc fingers typically found in repeats that are 3 bp apart and are found in diverse combinations in a variety of nucleic acid interacting proteins. TALEs on the other hand are found in repeats with a one-to-one recognition ratio between the amino acids and the recognized nucleotide pairs. Because both zinc fingers and TALEs happen in repeated patterns, different combinations can be tried to create a wide variety of sequence specificities. Approaches for making site-specific zinc finger endonucleases include, e.g., modular assembly (where Zinc fingers correlated with a triplet sequence are attached in a row to cover the required sequence), OPEN (low-stringency selection of peptide domains vs. triplet nucleotides followed by high-stringency selections of peptide combination vs. the final target in bacterial systems), and bacterial one-hybrid screening of zinc finger libraries, among others. ZFNs can also be designed and obtained commercially from e.g., Sangamo Biosciences™ (Richmond, Calif.). 
     Method for designing and obtaining TALENs are described in e.g. Reyon et al., 2012. Nature Biotechnology 30(5):460-5; Miller et al., 2011. Nat Biotechnol. 29:143-148; Cermak et al., 2011. Nucleic Acids Research 39 (12):e82 and Zhang et al., 2011 Nature Biotechnology 29 (2):149-53. A recently developed web-based program named Mojo Hand was introduced by Mayo Clinic for designing TAL and TALEN constructs for genome editing applications (can be accessed through www(dot)talendesign(dot)org). TALEN can also be designed and obtained commercially from e.g., Sangamo Biosciences™ (Richmond, Calif.). 
     The ZFN/TALEN system capability for precise targeting can be utilized for directing modifications in regulatory elements and/or coding regions upon introduction of the sequence of interest for trait improvement. 
     CRISPR/Cas9—The CRISPR/Cas system for genome editing contains two distinct components: a gRNA (guide RNA) and an endonuclease e.g. Cas9. 
     The gRNA is typically a 20-nucleotide sequence encoding a combination of the target homologous sequence (crRNA) and the endogenous bacterial RNA that links the crRNA to the Cas9 nuclease (tracrRNA) in a single chimeric transcript. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motif (PAM) sequence immediately following the target sequence. The binding of the gRNA/Cas9 complex localizes the Cas9 to the genomic target sequence so that the Cas9 can cut both strands of the DNA causing a double-strand break. Just as with ZFNs and TALENs, the double-stranded brakes produced by CRISPR/Cas can undergo homologous recombination or NHEJ. 
     The Cas9 nuclease has two functional domains: RuvC and HNH, each cutting a different DNA strand. When both of these domains are active, the Cas9 causes double strand breaks in the genomic DNA. 
     A significant advantage of CRISPR/Cas is that the high efficiency of this system coupled with the ability to easily create synthetic gRNAs enables multiple genes to be targeted simultaneously. In addition, the majority of cells carrying the mutation present biallelic mutations in the targeted genes. 
     However, apparent flexibility in the base-pairing interactions between the gRNA sequence and the genomic DNA target sequence allows imperfect matches to the target sequence to be cut by Cas9. 
     Modified versions of the Cas9 enzyme containing a single inactive catalytic domain, either RuvC- or HNH-, are called ‘nickases’. With only one active nuclease domain, the Cas9 nickase cuts only one strand of the target DNA, creating a single-strand break or ‘nick’. A single-strand break, or nick, is normally quickly repaired through the HDR pathway, using the intact complementary DNA strand as the template. However, two proximal, opposite strand nicks introduced by a Cas9 nickase are treated as a double-strand break, in what is often referred to as a ‘double nick’ CRISPR system. A double-nick can be repaired by either NHEJ or HDR depending on the desired effect on the gene target. Thus, if specificity and reduced off-target effects are crucial, using the Cas9 nickase to create a double-nick by designing two gRNAs with target sequences in close proximity and on opposite strands of the genomic DNA would decrease off-target effect as either gRNA alone will result in nicks that will not change the genomic DNA. 
     Modified versions of the Cas9 enzyme containing two inactive catalytic domains (dead Cas9, or dCas9) have no nuclease activity while still able to bind to DNA based on gRNA specificity. The dCas9 can be utilized as a platform for DNA transcriptional regulators to activate or repress gene expression by fusing the inactive enzyme to known regulatory domains. For example, the binding of dCas9 alone to a target sequence in genomic DNA can interfere with gene transcription. 
     There is a number of publically available tools available to help choose and/or design target sequences as well as lists of bioinformatically determined unique gRNAs for different genes in different species such as the Feng Zhang lab&#39;s Target Finder, the Michael Boutros lab&#39;s Target Finder (E-CRISP), the RGEN Tools: Cas-OFFinder, the CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomes and the CRISPR Optimal Target Finder. 
     In order to use the CRISPR system, both gRNA and Cas9 should be expressed in a target cell. The insertion vector can contain both cassettes on a single plasmid or the cassettes are expressed from two separate plasmids. CRISPR plasmids are commercially available such as the px330 plasmid from Addgene. 
     Recombinant adeno-associated virus (rAAV) platform—this genome-editing platform is based on rAAV vectors which enable insertion, deletion or substitution of DNA sequences in the genomes of live mammalian cells. The rAAV genome is a single-stranded deoxyribonucleic acid (ssDNA) molecule, either positive- or negative-sensed, which is about 4.7 kb long. These single-stranded DNA viral vectors have high transduction rates and have a unique property of stimulating endogenous homologous recombination in the absence of double-strand DNA breaks in the genome. One of skill in the art can design a rAAV vector to target a desired genomic locus and perform both gross and/or subtle endogenous gene alterations in a cell. rAAV genome editing has the advantage in that it targets a single allele and does not result in any off-target genomic alterations. rAAV genome editing technology is commercially available, for example, the rAAV GENESIS™ system from Horizon™ (Cambridge, UK). 
     Methods for qualifying efficacy and detecting sequence alteration are well known in the art and include, but not limited to, DNA sequencing, electrophoresis, an enzyme-based mismatch detection assay and a hybridization assay such as PCR, RT-PCR, RNase protection, in-situ hybridization, primer extension, Southern blot, Northern Blot and dot blot analysis. 
     Sequence alterations in a specific gene can also be determined at the protein level using e.g. chromatography, electrophoretic methods, immunodetection assays such as ELISA and Western blot analysis and immunohistochemistry. 
     In addition, one ordinarily skilled in the art can readily design a knock-in/knock-out construct including positive and/or negative selection markers for efficiently selecting transformed cells that underwent a homologous recombination event with the construct. Positive selection provides a means to enrich the population of clones that have taken up foreign DNA. Non-limiting examples of such positive markers include glutamine synthetase, dihydrofolate reductase (DHFR), markers that confer antibiotic resistance, such as neomycin, hygromycin, puromycin, and blasticidin S resistance cassettes. Negative selection markers are necessary to select against random integrations and/or elimination of a marker sequence (e.g. positive marker). Non-limiting examples of such negative markers include the herpes simplex-thymidine kinase (HSV-TK) which converts ganciclovir (GCV) into a cytotoxic nucleoside analog, hypoxanthine phosphoribosyltransferase (HPRT) and adenine phosphoribosytransferase (ARPT). 
     Recombination Procedures—Common to Different Genome Editing Systems 
     “Hit and run” or “in-out”—involves a two-step recombination procedure. In the first step, an insertion-type vector containing a dual positive/negative selectable marker cassette is used to introduce the desired sequence alteration. The insertion vector contains a single continuous region of homology to the targeted locus and is modified to carry the mutation of interest. This targeting construct is linearized with a restriction enzyme at a one site within the region of homology, electroporated into the cells, and positive selection is performed to isolate homologous recombinants. These homologous recombinants contain a local duplication that is separated by intervening vector sequence, including the selection cassette. In the second step, targeted clones are subjected to negative selection to identify cells that have lost the selection cassette via intrachromosomal recombination between the duplicated sequences. The local recombination event removes the duplication and, depending on the site of recombination, the allele either retains the introduced mutation or reverts to wild type. The end result is the introduction of the desired modification without the retention of any exogenous sequences. 
     The “double-replacement” or “tag and exchange” strategy—involves a two-step selection procedure similar to the hit and run approach, but requires the use of two different targeting constructs. In the first step, a standard targeting vector with 3′ and 5′ homology arms is used to insert a dual positive/negative selectable cassette near the location where the mutation is to be introduced. After electroporation and positive selection, homologous targeted clones are identified. Next, a second targeting vector that contains a region of homology with the desired mutation is electroporated into targeted clones, and negative selection is applied to remove the selection cassette and introduce the mutation. The final allele contains the desired mutation while eliminating unwanted exogenous sequences. 
     Site-Specific Recombinases—The Cre recombinase derived from the P1 bacteriophage and Flp recombinase derived from the yeast  Saccharomyces cerevisiae  are site-specific DNA recombinases each recognizing a unique 34 base pair DNA sequence (termed “Lox” and “FRT”, respectively) and sequences that are flanked with either Lox sites or FRT sites can be readily removed via site-specific recombination upon expression of Cre or Flp recombinase, respectively. For example, the Lox sequence is composed of an asymmetric eight base pair spacer region flanked by 13 base pair inverted repeats. Cre recombines the 34 base pair lox DNA sequence by binding to the 13 base pair inverted repeats and catalyzing strand cleavage and religation within the spacer region. The staggered DNA cuts made by Cre in the spacer region are separated by 6 base pairs to give an overlap region that acts as a homology sensor to ensure that only recombination sites having the same overlap region recombine. Basically, the site-specific recombinase system offers means for the removal of selection cassettes after homologous recombination. This system also allows for the generation of conditional altered alleles that can be inactivated or activated in a temporal or tissue-specific manner. Of note, the Cre and Flp recombinases leave behind a Lox or FRT “scar” of 34 base pairs. The Lox or FRT sites that remain are typically left behind in an intron or 3′ UTR of the modified locus, and current evidence suggests that these sites usually do not interfere significantly with gene function. Thus, Cre/Lox and Flp/FRT recombination involves introduction of a targeting vector with 3′ and 5′ homology arms containing the mutation of interest, two Lox or FRT sequences and typically a selectable cassette placed between the two Lox or FRT sequences. Positive selection is applied and homologous recombinants that contain targeted mutation are identified. Transient expression of Cre or Flp in conjunction with negative selection results in the excision of the selection cassette and selects for cells where the cassette has been lost. The final targeted allele contains the Lox or FRT scar of exogenous sequences. 
     Transposases—As used herein, the term “transposase” refers to an enzyme that binds to the ends of a transposon and catalyzes the movement of the transposon to another part of the genome. 
     As used herein the term “transposon” refers to a mobile genetic element comprising a nucleotide sequence which can move around to different positions within the genome of a single cell. In the process the transposon can cause mutations and/or change the amount of a DNA in the genome of the cell. A number of transposon systems that are able to also transpose in cells e.g. vertebrates have been isolated or designed, such as Sleeping Beauty [Izsvák and Ivics Molecular Therapy (2004) 9: 147-156], piggyBac [Wilson et al. Molecular Therapy (2007) 15: 139-145], Tol2 [Kawakami et al. PNAS (2000) 97 (21): 11403-11408] or Frog Prince [Miskey et al. Nucleic Acids Res. December 1, (2003) 31(23): 6873-6881]. Generally, DNA transposons translocate from one DNA site to another in a simple, cut-and-paste manner. Each of these elements has their own advantages, for example, Sleeping Beauty is particularly useful in region-specific mutagenesis, whereas Tol2 has the highest tendency to integrate into expressed genes. Hyperactive systems are available for Sleeping Beauty and piggyBac. Most importantly, these transposons have distinct target site preferences, and can therefore introduce sequence alterations in overlapping, but distinct sets of genes. Therefore, to achieve the best possible coverage of genes, the use of more than one element is particularly preferred. The basic mechanism is shared between the different transposases, therefore the piggyBac (PB) is described as an example. PB is a 2.5 kb insect transposon originally isolated from the cabbage looper moth,  Trichoplusia ni . The PB transposon consists of asymmetric terminal repeat sequences that flank a transposase, PBase. PBase recognizes the terminal repeats and induces transposition via a “cut-and-paste” based mechanism, and preferentially transposes into the host genome at the tetranucleotide sequence TTAA. Upon insertion, the TTAA target site is duplicated such that the PB transposon is flanked by this tetranucleotide sequence. When mobilized, PB typically excises itself precisely to reestablish a single TTAA site, thereby restoring the host sequence to its pretransposon state. After excision, PB can transpose into a new location or be permanently lost from the genome. Typically, the transposase system offers an alternative means for the removal of selection cassettes after homologous recombination quite similar to the use Cre/Lox or Flp/FRT. Thus, for example, the PB transposase system involves introduction of a targeting vector with 3′ and 5′ homology arms containing the mutation of interest, two PB terminal repeat sequences at the site of an endogenous TTAA sequence and a selection cassette placed between PB terminal repeat sequences. Positive selection is applied and homologous recombinants that contain targeted mutation are identified. Transient expression of PBase removes in conjunction with negative selection results in the excision of the selection cassette and selects for cells where the cassette has been lost. The final targeted allele contains the introduced mutation with no exogenous sequences. 
     For PB to be useful for the introduction of sequence alterations, there must be a native TTAA site in relatively close proximity to the location where a particular mutation is to be inserted. 
     Homology Directed Repair (HDR) Homology Directed Repair (HDR) can be used to generate specific nucleotide changes (also known as gene “edits”) ranging from a single nucleotide change to large insertions. In order to utilize HDR for gene editing, a DNA “repair template” containing the desired sequence must be delivered into the cell type of interest with e.g. the guide RNA [gRNA(s)] and Cas9 or Cas9 nickase or other genome editing method (examples herein below). The repair template must contain the desired edit as well as additional homologous sequence immediately upstream and downstream of the target (termed left and right homology arms). The length and binding position of each homology arm is dependent on the size of the change being introduced. The repair template can be a single stranded oligonucleotide, double-stranded oligonucleotide, or double-stranded DNA plasmid depending on the specific application. 
     The HDR method was successfully used for targeting a specific modification in a coding sequence of a gene in plants [Budhagatapalli Nagaveni et al. (2015) “Targeted Modification of Gene Function Exploiting Homology-Directed Repair of TALEN-Mediated Double-Strand Breaks in Barley”. G3 (Bethesda). 5(9): 1857-1863). Thus, the gfp-specific transcription activator-like effector nucleases were used along with a repair template that, via HDR, facilitates conversion of gfp into yfp, which is associated with a single amino acid exchange in the gene product. The resulting yellow-fluorescent protein accumulation along with sequencing confirmed the success of the genomic editing. 
     Similarly, Zhao Yongping et al. 2016 (An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design. Scientific Reports 6, Article number: 23890 (2016)) describe co-transformation of  Arabidopsis  plants with a combinatory dual-sgRNA/Cas9 vector that successfully deleted miRNA gene regions (MIR169a and MIR827a) and second construct that contains sites homologous to  Arabidopsis  TERMINAL FLOWER 1 (TFL1) for homology-directed repair (HDR) with regions corresponding to the two sgRNAs on the modified construct to provide both targeted deletion and donor repair for targeted gene replacement by HDR. 
     Specific considerations for Homology Directed Repair (HDR) utilizing CRISPR/Cas9 system are described herein: It should be noted that the repair template should not include a sequence that exhibits more than 90% identity to the gRNA designed to the genomic DNA or to the reverse complement sequence of the gRNA which is designed to the genomic sequence, otherwise the repair template becomes a suitable target for Cas9 cleavage. Additionally or alternatively, when using a short repair template (e.g., about 40-200 base pairs) the repair template should preferably lack the Protospacer Adjacent Motif (PAM) sequence. For example, the PAM could be mutated such that it is no longer present, but the coding region of the gene is not affected (i.e. a silent mutation). 
     Introduction of large double stranded DNA as repair template can be performed using plasmids, yet, the plasmid should be linearized before transfection. 
     Activation of Target Genes Using CRISPR/Cas9 System 
     Many bacteria and archaea contain endogenous RNA-based adaptive immune systems that can degrade nucleic acids of invading phages and plasmids. These systems consist of clustered regularly interspaced short palindromic repeat (CRISPR) genes that produce RNA components and CRISPR associated (Cas) genes that encode protein components. The CRISPR RNAs (crRNAs) contain short stretches of homology to specific viruses and plasmids and act as guides to direct Cas nucleases to degrade the complementary nucleic acids of the corresponding pathogen. Studies of the type II CRISPR/Cas system of  Streptococcus pyogenes  have shown that three components form an RNA/protein complex and together are sufficient for sequence-specific nuclease activity: the Cas9 nuclease, a crRNA containing 20 base pairs homologous to the target sequence, and a trans-activating crRNA (tracrRNA) (Jinek et al. Science (2012) 337: 816-821). It was further demonstrated that a synthetic chimeric guide RNA (gRNA) composed of a fusion between crRNA and tracrRNA could direct Cas9 to cleave DNA targets that are complementary to the crRNA in vitro. It was also demonstrated that transient expression of CRISPR-associated endonuclease (Cas9) in conjunction with synthetic gRNAs can be used to produce targeted double-stranded brakes in a variety of different species. 
     The CRISPR/Cas9 system is a remarkably flexible tool for genome manipulation. A unique feature of Cas9 is its ability to bind target DNA independently of its ability to cleave target DNA. Specifically, both RuvC- and HNH-nuclease domains can be rendered inactive by point mutations (D10A and H840A in SpCas9), resulting in a nuclease dead Cas9 (dCas9) molecule that cannot cleave target DNA. The dCas9 molecule retains the ability to bind to target DNA based on the gRNA targeting sequence. The dCas9 can be tagged with transcriptional activators, and targeting these dCas9 fusion proteins to the promoter region results in robust transcription activation of downstream target genes. The simplest dCas9-based activators consist of dCas9 fused directly to a single transcriptional activator. Importantly, unlike the genome modifications induced by Cas9 or Cas9 nickase, dCas9-mediated gene activation is reversible, since it does not permanently modify the genomic DNA. 
     Indeed, genome editing was successfully used to over-express a protein of interest in a plant by, for example, mutating a regulatory sequence, such as a promoter to overexpress the endogenous polynucleotide operably linked to the regulatory sequence. For example, U.S. Patent Application Publication No. 20160102316 to Rubio Munoz, Vicente et al., describes plants with increased expression of an endogenous DDA1 plant nucleic acid sequence wherein the endogenous DDA1 promoter carries a mutation introduced by mutagenesis or genome editing which results in increased expression of the DDA1 gene, using for example, CRISPR. The method involves targeting of Cas9 to the specific genomic locus, in this case DDA1, via a 20-nucleotide guide sequence of the single-guide RNA. An online CRISPR Design Tool can identify suitable target sites (tools.genome-engineering.org; Ran et al. (2013) Nature Protocols, 8911:2281-2308). 
     The CRISPR-Cas system was used for altering (increasing or decreasing) gene expression in plants as described in U.S. Patent Application publication No. 20150067922 to Yang; Yinong et al. The engineered, non-naturally occurring gene editing system comprises two regulatory elements, wherein the first regulatory element (a) operable in a plant cell operably linked to at least one nucleotide sequence encoding a CRISPR-Cas system guide RNA (gRNA) that hybridizes with the target sequence in the plant, and a second regulatory element (b) operable in a plant cell operably linked to a nucleotide sequence encoding a Type-II CRISPR-associated nuclease, wherein components (a) and (b) are located on same or different vectors of the system, whereby the guide RNA targets the target sequence and the CRISPR-associated nuclease cleaves the DNA molecule, thus altering the expression of a gene product in a plant. It should be noted that the CRISPR-associated nuclease and the guide RNA do not naturally occur together. 
     In addition, as described above, point mutations which activate a gene-of-interest and/or which result in over-expression of a polypeptide-of-interest can be also introduced into plants by means of genome editing. Such mutation can be for example, deletions of repressor sequences which result in activation of the gene-of-interest; and/or mutations which insert nucleotides and result in activation of regulatory sequences such as promoters and/or enhancers. 
     According to additional aspect, the present invention provides a genetically engineered plant having enhanced resistance to at least one fungus and/or Oomycete compared to a non-engineered control plant, the genetically engineered plant comprises at least one cell having modified expression and/or activity of at least one polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252 or at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506 and 2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof compared to the polypeptide expression and/or activity in the non-engineered control plant. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having modified expression and/or activity of at least one polypeptide having the amino acid sequences selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508 and 2635, 2444-2634, 2636-4252. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant having enhanced resistance to the at least one fungus and/or Oomycete comprises at least one cell with enhanced expression and/or activity of at least one polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs: 2635, 2444-2634, 2636-4252 or at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-506. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell transformed with an exogenous polynucleotide encoding the at least one polypeptide, the polynucleotide is at least 70% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443 or at least 80% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41, 43-61, 63-79, 81, and 83-301, thereby having an enhanced resistance to the at least one fungus. The exogenous polynucleotide can be endogenous to the plant cell or heterologous to the plant cell. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell edited to express an exogenous polynucleotide encoding the at least one polypeptide, thereby having an enhanced resistance to the at least one fungus. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell edited to over-express an endogenous polynucleotide encoding the at least one polypeptide, thereby having an enhanced resistance to the at least one fungus. 
     According to certain embodiments, the genetically engineered plant having enhanced resistance to the at least one fungus comprises at least one cell with enhanced expression of at least one polynucleotide encoding a polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252 or at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-506. According to certain exemplary embodiments, the polynucleotide expression in the genetically engineered plant is enhanced in comparison to the polynucleotide expression in a control plant. 
     According to certain embodiments, the genetically engineered plant having enhanced resistance to the at least one fungus comprises at least one cell with reduced expression and/or activity of the at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:328, 348 and 361. According to certain exemplary embodiments, the polypeptide expression and/or activity in the genetically engineered plant is reduced in comparison to the polypeptide expression and/or activity in a control plant. 
     According to certain embodiments, the genetically engineered plant having reduced expression and/or activity of the at least one polypeptide comprises at least one cell having reduced expression of a polynucleotide encoding said at least one polypeptide, thereby having an enhanced resistance to the at least one fungus. 
     According to certain embodiments, the genetically engineered plant comprises a polynucleotide encoding a modified form of the at least one polypeptide, wherein the modified form has reduced or no activity compared to the unmodified form, thereby having an enhanced resistance to the at least one fungus. 
     According to additional aspect, the present invention provides a genetically engineered plant having enhanced resistance to at least one fungus and/or Oomycete compared to a control plant, the genetically engineered plant comprises at least one cell having modified expression and/or activity of at least one polypeptide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous, or identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-506 and 2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof compared to the polypeptide expression and/or activity in a control plant. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having modified expression and/or activity of at least one polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327-359, 361-508 and 2635, 2444-2634, 2636-4252. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having modified expression of a polynucleotide encoding the at least one polypeptide. 
     According to additional aspect, the present invention provides a genetically engineered plant having enhanced resistance to at least one fungus and/or Oomycete compared to a control plant, the genetically engineered plant comprises at least one cell having modified expression of at least one polynucleotide at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79% or more homologous to a polynucleotide having a nucleic acid sequence selected from the group consisting of SEQ ID NOs:783, 592-782, 784-2443; or at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% or more homologous, or identical to a polynucleotide having an nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, 81-301 and 783, 592-782, 784-2443 compared to the polynucleotide expression and/or activity in a control plant. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, the genetically engineered plant comprises at least one cell having modified expression of at least one polynucleotide having the nucleic acid sequence selected from the group consisting of SEQ ID NOs:40, 80, 22-39, 41-79, 81-305 and 783, 592-782, 784-2443. Each possibility represents a separate embodiment of the present invention. 
     According to certain embodiments, modified expression/and or activity of a polypeptide or polynucleotide encoding same comprises enhanced expression and/or activity. According to certain embodiments, modified expression/and or activity of a polypeptide or polynucleotide encoding same comprises reduced expression and/or activity. 
     According to certain embodiments, the genetically engineered plant having enhanced resistance to the at least one fungus and/or Oomycete comprises at least one cell with enhanced expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:326, 360, 308-325, 327, 329-347, 349-359, 362-506 and 2635, 2444-2634, 2636-4252. According to certain exemplary embodiments, the polypeptide expression and/or activity in the genetically engineered plant is enhanced in comparison to the polypeptide expression and/or activity in a control plant. 
     According to certain embodiments, the genetically engineered plant having enhanced resistance to the at least one fungus and/or Oomycete comprises at least one cell with reduced expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:328, 361 and 348. According to certain exemplary embodiments, the polypeptide expression and/or activity in the genetically engineered plant is reduced in comparison to the polypeptide expression and/or activity in a control plant. 
     According to certain embodiments, the genetically engineered plant having reduced expression and/or activity of the at least one polypeptide comprises at least one cell having reduced expression of a polynucleotide encoding said at least one polypeptide, thereby having an enhanced resistance to the at least one fungus. 
     According to certain embodiments, the genetically engineered plant comprises a polynucleotide encoding a modified form of the at least one polypeptide, wherein the modified form has reduced or no activity compared to the unmodified form, thereby having an enhanced resistance to the at least one fungus. 
     Once expressed within the plant cell or the entire plant, the level of the polypeptide encoded by the exogenous polynucleotide can be determined by methods well known in the art such as, activity assays, Western blots using antibodies capable of specifically binding the polypeptide, Enzyme-Linked Immuno Sorbent Assay (ELISA), radio-immuno-assays (RIA), immunohistochemistry, immunocytochemistry, immunofluorescence and the like. 
     Methods of determining the level in the plant of the RNA transcribed from the exogenous polynucleotide are well known in the art and include, for example, Northern blot analysis, reverse transcription polymerase chain reaction (RT-PCR) analysis (including quantitative, semi-quantitative or real-time RT-PCR) and RNA-in situ hybridization. 
     The sequence information and annotations uncovered by the present teachings can be harnessed in favor of classical breeding. Thus, sub-sequence data of those polynucleotides described above, can be used as markers for marker assisted selection (MAS), in which a marker is used for indirect selection of a genetic determinant or determinants of a trait of interest (e.g., biomass, growth rate, oil content, yield, abiotic stress tolerance, water use efficiency, nitrogen use efficiency and/or fertilizer use efficiency). Nucleic acid data of the present teachings (DNA or RNA sequence) may contain or be linked to polymorphic sites or genetic markers on the genome such as restriction fragment length polymorphism (RFLP), microsatellites and single nucleotide polymorphism (SNP), DNA fingerprinting (DFP), amplified fragment length polymorphism (AFLP), expression level polymorphism, polymorphism of the encoded polypeptide and any other polymorphism at the DNA or RNA sequence. 
     Examples of marker assisted selections include, but are not limited to, selection for a morphological trait (e.g., a gene that affects form, coloration, male sterility or resistance such as the presence or absence of awn, leaf sheath coloration, height, grain color, aroma of rice); selection for a biochemical trait (e.g., a gene that encodes a protein that can be extracted and observed; for example, isozymes and storage proteins); selection for a biological trait (e.g., pathogen races or insect biotypes based on host pathogen or host parasite interaction can be used as a marker since the genetic constitution of an organism can affect its susceptibility to pathogens or parasites). 
     The polynucleotides and polypeptides described hereinabove can be used in a safe and cost-effective manner in a wide range of economical plants, exemplary species of which are described hereinabove. 
     It will be appreciated that some genes involved in a plant defense mechanism conferring resistance to a particular fungus species may also be involved in resistance to other species, regulated by the same or homologous genes. Of course, the overall defense mechanism is related, not identical, and therefore not all genes involved in resistance to one pathogen will confer resistance to other pathogens. Nonetheless, if a gene confers or enhances resistance to one of the pathogen species, it would be apparent to one skilled in the art to test for resistance to other pathogens, specifically to pathogen of the same genus or that cause similar symptoms. 
     According to certain embodiments, the fungus and/or Oomycete is selected from, but not limited to,  Fusarium verticillioides; Fusarium graminearum; Collotetrichum graminicola; Fusarium avenaceum; Fusarium culmorum; Fusarium oxysporum; Fusarium roseum; Fusarium semitectum; Fusarium solani; Fusarium verticillioides; Fusarium verticillioides  var.  subglutinans; Acremonium strictum; Albugo candida; Albugo tragopogonis; Alternaria alternate; Alternaria brassicae; Alternaria helianthi; Alternaria zinnia; Aphanomyces euteiches; Ascochyta sorghina; Ascochyta tritici; Aspergillus flavus; Bipolaris maydis  O;  Bipolaris sorghicola; Bipolaris sorokiniana; Botrytis cinerea; Cephalosporium acremonium; Cephalosporium gramineum; Cephalosporium maydis; Cercospora kikuchii; Cercospora medicaginis; Cercospora sojina; Cercospora sorghi; Cladosporium herbarum; Clavibacter michiganense  subsp.  Nebraskense; Clavibacter michiganese  subsp.  Insidiosum; Claviceps purpurea; Claviceps sorghi; Cochliobolus heterostrophus; Colletotrichum dematium  ( Colletotichum truncatum );  Colletotrichum trifolii; Colletotrichum sublineolum ; Corn stunt spiroplasma;  Corynespora cassiicola; Curvularia inaequalis; Curvularia lunata; Curvularia pallescens; Diaporthe phaseolorum  var.  caulivora; Diaporthe phaseolorum  var.  sojae  ( Phomopsis sojae );  Diplodia macrospora; Erwinia carotovora; Erwinia carotovorum  pv.  Carotovora; Erwinia chrysanthemi  pv.  Zea; Erwinia stewartii; Erysiphe cichoracearum; Erysiphe graminis  fsp.  tritici; Exserohilum turcicum  I, II &amp; III;  Gaeumannomyces graminis  var.  tritici; Gibberella zeae  ( Fusarium graminearum );  Gloeocercospora sorghi; Glomerella glycines; Helminthosporium carbonum  I, II &amp; III ( Cochliobolus carbonum );  Helminthosporium pedicellatum; Helminthosporium sorghicola; Kabatiella maydis; Leptosphaeria maculans; Leptosphaerulina briosiana; Leptotrichila medicaginis; Macrophomina phaseolina; Microsphaera diffusa; Mycosphaerella brassicicola; Nigrospora oryzae; Penicillium oxalicum; Perconia circinata; Peronosclerospora maydis; Peronosclerospora philippinensis; Peronosclerospora sacchari; Peronosclerospora sorghi; Peronospora manshurica; Peronospora parasitica; Peronospora trifoliorum; Phakopsora pachyrhizi; Phialophora gregata; Phoma insidiosa; Phoma macdonaldii; Phoma medicaginis  var.  medicaginis; Phomopsis helianthi; Phyllachara sacchari; Phyllosticta maydis; Phyllosticta sojicola; Physoderma maydis; Physopella zeae; Phytophthora cryptogea; Phytophthora megasperma; Phytophthora megasperma  fsp.  Glycinea; Plasmopora halstedii; Pseudocercosporella herpotrichoides; Pseudomonas andropogonis; Pseudomonas avenae; Pseudomonas avenae  ( Pseudomonas alboprecipitans );  Pseudomonas syringae  p.v.  atrofaciens; Pseudomonas syringae  p. v.  glycinea; Pseudomonas syringae  p. v.  syringae; Pseudopeziza medicaginis; Puccinia graminis  fsp.  tritici; Puccinia helianthi; Puccinia polysora; Puccinia purpurea; Puccinia recondita  fsp.  tritici; Puccinia sorghi; Puccinia striiformis; Pyrenophora tritici - repentis; Pythium aphanidermatum; Pythium arrhenomanes; Pythium debaryanum; Pythium gramicola; Pythium graminicola; Pythium irregular; Pythium splendens; Pythium ultimum; Ramulispora sorghi; Ramulispora sorghicola; Rhizoctonia cerealis; Rhizoctonia solani; Rhizopus arrhizus; Rhizopus oryzae; Rhizopus stolonifera; Sclerophthona macrospora; Sclerospora graminicola; Sclerotinia sclerotiorum; Sclerotinia trifoliorum; Sclerotium rolfsii; Septoria avenae; Septoria glycines; Septoria helianthi; Septoria nodorum; Septoria tritici; Exserohilum turcicum; Sphacelotheca cruenta; Sporisorium reilianum  ( Sphacelotheca reiliana );  Sporisorium sorghi; Stagonospora meliloti; Stemphylium alfalfa; Stemphylium botryosum; Stemphylium herbarum; Stenocarpella maydi  ( Diplodia maydis );  Tilletia indica; Tilletia laevis; Tilletia tritici; Trichoderma viride; Urocystis agropyri; Uromyces striatus; Ustilago maydis; Ustilago tritici; Verticillium albo - atrum; Verticillium dahlia; Xanthomonas campestris  p.v.  alfalfa; Xanthomonas campestris  p. v.  hokicola; Xanthomonas campestris  p. v.  phaseoli ; and  Xanthomonas campestris  p.v.  translucens . Each possibility represents a separate embodiment of the present invention. 
     Specific pathogenic fungi or Oomycetes are known to cause dramatic crop lose due to disease symptoms which negatively affect the quality of the crop. For example,  Fusarium verticilloides  and  Fusarium graminearum  cause rot in maize (specifically stalk rot), wheat, sweet paper, eggplants and head blight is wheat.  Fusarium oxysporum  causes sudden death syndrome (SDS) in soybeans, yellow spots in sugar beet, Panama disease in Banana, and wilt in tomato, sweet pepper, eggplants, potatoes and various plant of the Cucurbitaceae family.  Colletotrichum  spp. cause stalk rot in maize, anthracnose in sugar beet, tomato and sweet pepper.  Botrytis cinerea  causes gray mold in tomato, sweet pepper, eggplants and potato. Rust is caused by  Puccinia  spp. in maize, wheat and sunflower, by  Uromyces  spp. in sunflower and by  Phakopsora  in soybean.  Phytophthora  causes root rot in soybean, late blight in tomato and potato, blight in eggplant and blight fruit rot in sweet pepper.  Mycosphaerella graminicola  causes leaf blotch in wheat.  Mycosphaerella fijiensis  causes black leaf streak disease (BLSD; aka black Sigatoka leaf spot) in banana.  Septoria lycopersici  causes leaf spots in tomato.  Verticillium  spp. cause wilt disease in canola, sugar beet, tomato, sweet pepper, eggplant and potato.  Magnaporthe oryza  causes rice blast.  Pythium  spp. cause damping off disease in maize, soybean, tomato, sweet pepper, eggplant and potato and black vessels in sugar beet.  Sclerotinia  causes stem rot in soybean and white mold in tomato, sweet pepper, eggplant and potato.  Rhizoctonia solani  causes root crown rot in sugar beet, sheath blight in rice, and damping off disease in tomato, sweet pepper, eggplant and potato. Maize smut is caused by  Ustilago maydis. Alternaria  spp. cause leaf spots in sugar beet and sweet pepper, early blight in tomato and potato, and fruit rot in sweet pepper and eggplants.  Cercospora  causes leaf blight in soybean and leaf spots in sugar beet, sweet pepper, eggplants and potato.  Macrophomina  causes charcoal rot in maize, wheat, soybean, tomato and potato.  Sclerotium rolfsii  causes Southern blight in sweet pepper and eggplants.  Oidium  spp. cause powdery mildew in tomato, sweet pepper, eggplants and potato. Powdery mildew is also caused by  Blumeria graminis.    
     Methods for identifying symptoms caused by various fungi and Oomycetes upon infection of specific plant species, and for measuring the degree of the plant susceptibility/resistance to the infection are well known to those skilled in the art. 
     The term “plant” as used herein encompasses a whole plant, a grafted plant, ancestor(s) and progeny of the plants and plant parts, including seeds, shoots, stems, roots (including tubers), rootstock, scion, and plant cells, tissues and organs. The plant or part thereof may be in any form including suspension cultures, embryos, meristematic regions, callus tissue, leaves, gametophytes, sporophytes, pollen, and microspores. Plants that are particularly useful in the methods of the invention include all plants which belong to the superfamily Viridiplantae, in particular monocotyledonous and dicotyledonous plants including a fodder or forage legume, ornamental plant, food crop, tree, or shrub selected from the list comprising Acacia spp., Acer spp.,  Actinidia  spp.,  Aesculus  spp., Agathis  australis, Albizia amara, Alsophila tricolor, Andropogon  spp.,  Arachis  spp,  Areca catechu, Astelia fragrans, Astragalus cicer, Baikiaea plurijuga, Betula  spp.,  Brassica  spp.,  Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadaba farinosa, Calliandra  spp,  Camellia sinensis, Canna indica, Capsicum  spp.,  Cassia  spp.,  Centroema pubescens, Chacoomeles  spp.,  Cinnamomum cassia, Coffea arabica, Colophospermum mopane, Coronillia varia, Cotoneaster serotina, Crataegus  spp.,  Cucumis  spp.,  Cupressus  spp.,  Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogon  spp.,  Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davallia divaricata, Desmodium  spp.,  Dicksonia squarosa, Dibeteropogon amplectens, Dioclea  spp,  Dolichos  spp.,  Dorycnium rectum, Echinochloa pyramidalis, Ehraffia  spp.,  Eleusine coracana, Eragrestis  spp.,  Erythrina  spp.,  Eucalypfus  spp.,  Euclea schimperi, Eulalia villosa, Pagopyrum  spp.,  Feijoa sellowlana, Fragaria  spp.,  Flemingia  spp,  Freycinetia banksli, Geranium thunbergii, Ginkgo biloba, Glycine javanica, Gliricidia  spp,  Gossypium hirsutum, Grevillea  spp.,  Guibourtia coleosperma, Hedysarum  spp.,  Hemaffhia altissima, Heteropogon contoffus, Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffhelia dissolute, Indigo incamata, Iris  spp.,  Leptarrhena pyrolifolia, Lespediza  spp.,  Lettuca  spp.,  Leucaena leucocephala, Loudetia simplex, Lotonus bainesli, Lotus  spp.,  Macrotyloma axillare, Malus  spp.,  Manihot esculenta, Medicago saliva, Metasequoia glyptostroboides, Musa sapientum, Nicotianum  spp.,  Onobrychis  spp.,  Ornithopus  spp.,  Oryza  spp.,  Peltophorum africanum, Pennisetum  spp.,  Persea gratissima, Petunia  spp.,  Phaseolus  spp.,  Phoenix canariensis, Phormium cookianum, Photinia  spp.,  Picea glauca, Pinus  spp.,  Pisum sativam, Podocarpus totara, Pogonarthria fleckii, Pogonaffhria squarrosa, Populus  spp.,  Prosopis cineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis, Quercus  spp.,  Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhus natalensis, Ribes grossularia, Ribes  spp.,  Robinia pseudoacacia, Rosa  spp.,  Rubus  spp.,  Salix  spp.,  Schyzachyrium sanguineum, Sciadopitys vefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghum bicolor, Spinacia  spp.,  Sporobolus fimbriatus, Stiburus alopecuroides, Stylosanthos humilis, Tadehagi  spp,  Taxodium distichum, Themeda triandra, Trifolium  spp.,  Triticum  spp.,  Tsuga heterophylla, Vaccinium  spp.,  Vicia  spp.,  Vitis vinifera, Watsonia pyramidata, Zantedeschia aethiopica, Zea mays , amaranth, artichoke, asparagus, broccoli, Brussels sprouts, cabbage, canola, carrot, cauliflower, celery, collard greens, flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean, straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize, wheat, barley, rye, oat, peanut, pea, lentil and alfalfa, cotton, rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, a tree, an ornamental plant, a perennial grass and a forage crop. Alternatively, algae and other non-Viridiplantae can be used for the methods of the present invention. 
     According to some embodiments, the plant used according to the teachings of the present invention is a crop plant such as maize, rice, wheat, barley, peanut, potato, sesame, olive tree, palm oil, banana, soybean, sunflower, canola, sugarcane, alfalfa, millet, Leguminosae (bean, pea), flax, lupinus, rapeseed, tobacco, poplar and cotton. According to certain exemplary embodiments, the crop plant is maize ( Zea mays ). 
     According to some embodiment, the plant used according to the teachings of the present invention is a field crop plant selected from the group consisting of tomato, potato, sweet potato, cassava, beets, ginger, horseradish, radish, ginseng, turnip, any root or tuber crop, pepper, eggplant, ground cherry, tomatillo, okra, other fruiting vegetables, cucumber cantaloupe, melon, muskmelon, squash, watermelon and other cucurbit plants. 
     According to some embodiments of the invention the plant is a dicotyledonous plant. 
     According to some embodiments of the invention the plant is a monocotyledonous plant. 
     According to some embodiments the present invention provides a plant cell expressing the exogenous polynucleotide of some embodiments of the invention, the nucleic acid construct comprising the exogenous polynucleotide of some embodiments of the invention and/or the polypeptide of some embodiments of the invention. 
     According to a further aspect, the present invention provides a fungicidal composition comprising an effective amount of at least one polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof, wherein the fungicidal composition is effective in inhibiting the growth and/or development of at least one plant pathogenic fungi and/or oomycetes. According to certain embodiments, the fungicidal composition comprises at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 4468, 4661, 2444-2634, 2636-4252, 4450-4467, 4469-4660, and 4662-4799. According to some embodiments, the fungicidal composition comprises at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:4468, 4661, 4450-4467, 4469-4660, and 4662-4799. 
     According to yet additional aspect, the present invention provides a fungicidal composition comprising bacteria comprising at least one polypeptide at least 70% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 2444-2634, 2636-4252, a fragment and/or a variant thereof, wherein the fungicidal composition is effective in inhibiting the growth and/or development of at least one plant pathogenic fungi and/or oomycetes. According to certain embodiments, the bacteria comprise at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:2635, 4468, 4661, 2444-2634, 2636-4252, 4450-4467, 4469-4660, and 4662-4799. According to some embodiments, the bacteria comprise at least one polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:4468, 4661, 4450-4467, 4469-4660, and 4662-4799. Each possibility represents a separate embodiment of the present invention. The bacteria can be of the same species or of different species, with the proviso that the bacteria have no adverse effect on the plant. The bacteria can be alive or dead. Any bacteria suitable for expression of exogenous genes and not having negative effects on plant can be used (see, e.g. Billman-Jacobe H. “Expression in bacteria other than  E. coli  1996. Curr. Opiniom in Biotech 7:500). According to certain exemplary embodiments, the bacteria are selected from the group consisting of  Bacillus subtilis, Lactococcus lacti, Corynebacterium glutamicum ; and  Bacillus brevis . Particular example is  Bacillus  Expression systems Lonza XS™ ToolBox. 
     According to certain embodiments, the fungicidal composition further comprises at least one agriculturally compatible carrier or diluent. 
     It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination or as suitable in any other described embodiment of the invention. Certain features described in the context of various embodiments are not to be considered essential features of those embodiments, unless the embodiment is inoperative without those elements. 
     The following examples are presented in order to more fully illustrate some embodiments of the invention. They should, in no way be construed, however, as limiting the broad scope of the invention. One skilled in the art can readily devise many variations and modifications of the principles disclosed herein without departing from the scope of the invention. 
     EXAMPLES 
     Reference is now made to the following examples, which together with the above descriptions illustrate some embodiments of the invention in a non-limiting fashion. 
     Generally, the nomenclature used herein and the laboratory procedures utilized in the present invention include molecular, biochemical, microbiological and recombinant DNA techniques. Such techniques are thoroughly explained in the literature. See, for example, “Molecular Cloning: A laboratory Manual” Sambrook et al., (1989); “Current Protocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed. (1994); Ausubel et al., “Current Protocols in Molecular Biology”, John Wiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide to Molecular Cloning”, John Wiley &amp; Sons, New York (1988); Watson et al., “Recombinant DNA”, Scientific American Books, New York; Birren et al. (eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, Cold Spring Harbor Laboratory Press, New York (1998); methodologies as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and 5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis, J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-III Coligan J. E., ed. (1994); Stites et al. (eds), “Basic and Clinical Immunology” (8th Edition), Appleton &amp; Lange, Norwalk, Conn. (1994); Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W. H. Freeman and Co., New York (1980); “Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic Acid Hybridization” Hames, B. D., and Higgins S. J., eds. (1985); “Transcription and Translation” Hames, B. D., and Higgins S. J., Eds. (1984); “A Practical Guide to Molecular Cloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317, Academic Press; “PCR Protocols: A Guide To Methods and Applications”, Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategies for Protein Purification and Characterization—A Laboratory Course Manual” CSHL Press (1996); all of which are incorporated by reference as if fully set forth herein. Other general references are provided throughout this document. The procedures therein are believed to be well known in the art and are provided for the convenience of the reader. All the information contained therein is incorporated herein by reference. 
     Section I: Plant Genes Associated with Fungal Resistance 
     Example 1: Identification of Plant Genes Associated with Fungal Resistance 
     The inventors of the present invention have identified polynucleotides related to resistant to fungal infection, particularly to infection by  Fusarium verticillioides, Fusarium graminearum  or  Colletotrichum graminicola . Modulating the expression of the polynucleotide in plants can increase the plant resistance to fungal infection. 
     The nucleotide sequence datasets used for the polynucleotide identification were originated from publicly available databases as well as from Applicant proprietary sequencing data obtained using the Solexa technology (form e.g. wheat, maize and sorghum). Sequence data from 200 different plant species was introduced into a single, comprehensive database. The information used to build the datasets included gene expression levels, protein annotation, enzymatic activity and involvement in biosynthetic pathways. 
     Major databases used included: 
     Genomic Databases 
       Arabidopsis  genome [TAIR genome version 6 (arabidopsis.org/)]; 
     Rice genome [IRGSP build 4.0 (rgp.dna.affrc.go.jp/IRGSP/)]; 
     Poplar [ Populus trichocarpa  release 1.1 from JGI (assembly release v1.0) (genome.jgi-psf.org/)]; 
       Brachypodium  [JGI 4× assembly, brachpodium.org)]; 
     Soybean [DOE-JGI SCP, version Glyma0 (phytozome.net/)]; 
     Grape [French-Italian Public Consortium for Grapevine Genome Characterization grapevine genome (genoscope.cns.fr/)]; 
     Castorbean [TIGR/J Craig Venter Institute 4× assembly [msc.jcvi.org/r communis]; 
     Sorghum [DOE-JGI SCP, version Sbi1 [phytozome.net/)]; and 
     Maize “B73” [DOE-JGI SCP, version AGPv2 [phytozome.net/)]; 
     Databases of Expressed Sequence Tag (EST) and mRNA Sequences: 
     GenBank ncbi.nlm.nih.gov/dbEST; 
     RefSeq (ncbi.nlm.nih.gov/RefSeq/); 
     TAIR (arabidopsis.org/); 
     Protein and Pathway Databases: 
     Uniprot [uniprot.org/]; 
     AraCyc [arabidopsis.org/biocyc/index.jsp]; 
     ENZYME [expasy.org/enzyme/]; 
     Microarray datasets were downloaded from: GEO (ncbi.nlm.nih.gov/geo/); TAIR (arabidopsis.org/); and Applicant proprietary microarray data as described in PCT Patent Application Publication No. WO 2008/122980. 
     QTL (quantitative trait loci) and SNPs information was retrieved from Gramene [gramene.org/qtl/]; and Panzea [panzea.org/index.html]. 
     Database Assembly 
     Database assembly was performed to build a wide, rich, reliable annotated and easy to analyze database. The assembly comprised data retrieved from publicly available genomic sequences, mRNA sequences, ESTs DNA sequences and QTL data, as well as information regarding gene expression, protein annotation, and involvement in biosynthesis pathway, all in various plant types. The assembly further comprised data retrieved from Applicant proprietary databases produced from various plant types including genomic sequences, mRNA sequences, expression, proteomic and metabolomic data, QTL and GWAS (genome-wide association studies) data. 
     Database assembly is comprised of a toolbox of gene refining, structuring and annotation as well as analysis tools enabling to construct a tailored database for each gene-discovery project. Gene refining and structuring tools enable to reliably detect splice variants and antisense transcripts, and understand various potential phenotypic outcomes of a single gene. The capabilities of the “LEADS” platform of Compugen LTD. for analyzing human genome have been confirmed and accepted by the scientific community [see e.g., “Widespread Antisense Transcription”, Yelin, et al. (2003) Nature Biotechnology 21, 379-85; “Splicing of Alu Sequences”, Lev-Maor, et al. (2003) Science 300 (5623), 1288-91; “Computational analysis of alternative splicing using EST tissue information”, Xie H et al. Genomics 2002], and have been proven most efficient in plant genomics as well. 
     EST Clustering and Gene Assembly 
     For gene clustering and assembly of organisms with available genome sequence data ( Arabidopsis , rice, castorbean, grape,  Brachypodium , poplar, soybean, sorghum, maize) the genomic LEADS version (GANG) was employed. This tool allows most accurate clustering of ESTs and mRNA sequences on genome, and predicts gene structure as well as alternative splicing events and anti sense transcription. 
     For organisms with no available full genome sequence data, “expressed LEADS” clustering software was applied. 
     Gene Annotation 
     Predicted genes and proteins were annotated as follows: BLAST™ search [blast.ncbi.nlm.nih.gov/Blast.cgi] against all plant UniProt [uniprot.org/] sequences was performed. Open reading frames (ORFs) of each putative transcript were analyzed and longest ORF with highest number of homologues was selected as a predicted protein of the transcript. The predicted proteins were analyzed by InterPro [ebi.ac.uk/interpro/]. 
     BLAST™ against proteins from AraCyc and ENZYME databases was used to map the predicted transcripts to AraCyc pathways. 
     Predicted proteins from different species were compared using BLAST™ algorithm (ncbi.nlm.nih.gov/Blast.cgi) to validate the accuracy of the predicted protein sequence, and for efficient detection of orthologs. 
     Gene Expression Profiling 
     Several data sources were exploited for gene expression profiling, namely microarray data and digital expression profile (see below). Genes were analyzed for expression patterns in different plant species and varieties. The analysis was based on differential expression under uninfected and infected conditions, wherein the infection was induced by different pathogens and the gene expression was measured in different plant organs and at different time points along the disease development. Publicly available microarray datasets were downloaded from TAIR and NCBI GEO sites, renormalized, and integrated into the database. Expression profiling is one of the most important resource data for identifying genes related to disease resistance. 
     A digital expression profile summary was compiled for each gene cluster according to all keywords included in the sequence records comprising the cluster. Digital expression, also known as electronic Northern Blot, is a tool that displays virtual expression profile based on the expressed sequence tag (EST) sequences forming the gene cluster. The tool provides the expression profile of a cluster in terms of plant anatomy (e.g., the tissue/organ in which the gene is expressed), developmental stage (the developmental stages at which a gene can be found) and profile of treatment (provides the physiological conditions under which a gene is expressed such as drought, cold, pathogen infection, etc.). Given a random distribution of ESTs in the different clusters, the digital expression provides a probability value that describes the probability of a cluster having a total of N ESTs to contain X ESTs from a certain collection of libraries. For the probability calculations, the following is taken into consideration: a) the number of ESTs in the cluster, b) the number of ESTs of the implicated and related libraries, c) the overall number of ESTs available representing the species. Thereby clusters with low probability values are highly enriched with ESTs from the group of libraries of interest indicating a specialized expression. 
     Recently, the accuracy of this system was demonstrated by Portnoy et al., 2009 (Analysis of The Melon Fruit Transcriptome Based on 454 Pyrosequencing, in: Plant &amp; Animal Genomes XVII Conference, San Diego, Calif.). Transcriptomic analysis, based on relative EST abundance in data was performed by 454 pyrosequencing of cDNA representing mRNA of the melon fruit. Fourteen double strand cDNA samples obtained from two genotypes, two fruit tissues (flesh and rind) and four developmental stages were sequenced. GS FLX pyrosequencing (Roche/454 Life Sciences) of non-normalized and purified cDNA samples yielded 1,150,657 expressed sequence tags that assembled into 67,477 unigenes (32,357 singletons and 35,120 contigs). Analysis of the data obtained against the Cucurbit Genomics Database [icugi.org/] confirmed the accuracy of the sequencing and assembly. Expression patterns of selected genes fitted well their qRT-PCR data. 
     The produced datasets were used for further comparative analyses of gene expression in various plant species and lines in response to fungal infection. 
     Example 2: Production of Plant Transcriptomes for Discovery of Genes Correlating with Resistance to the Fungal Infection 
     Experimental Procedures 
     The association of gene expression with fungal infection was examined in  Brachypodium distachyon, Aegilops  spp.,  Setaria italica, Triticum aestivum  and  Zea mays.    
     The following pathogenic fungi were used:  Fusarium verticillioides  (hereinafter  F. verticillioides  or Fv). A GFP transformant of  F. verticillioides  strain A-00149-FGSC 7600 (Oren et al., 2003, Appl. Environ. Microbiol. 69:1695) was used throughout the data generation and validation experiments; and  Fusarium graminearum  (hereinafter  F. graminearum  or Fg). 
     Fungi Infection 
     Fungal infection of plant roots: this method was used for inoculating plant root with  F. verticillioides  (Fv) and  Fusarium graminearum  (Fg). Sterilized seeds from tolerant and sensitive lines of the examined plant were germinated on water agar supplemented with Ampicillin (100 μg/ml) and inoculated after 6 days with an agar plug (1 cm diameter) covered with one-week old fungal mycelium applied to the root site (1 cm below the seed) for 2 hours four days after germination. Control plants were mock inoculated with an empty plug. 
     RNA extraction was performed using TRIzol Reagent from Invitrogen (invitrogen.com/content.cfm?pageid=469). Approximately 30-50 mg of sample tissue was taken for the analysis. The weighed tissues were ground using pestle and mortar in liquid nitrogen and resuspended in 500 μl of TRIzol Reagent. To the homogenized lysate, 100 μl of chloroform were added followed by precipitation using isopropanol and two washes with 75% ethanol. The RNA was eluted in 30 μl of RNase-free water. RNA samples were cleaned up using Qiagen&#39;s RNeasy minikit clean-up protocol as per the manufacturer&#39;s protocol (QIAGEN Inc, CA USA). 
     RNA was extracted from tissues of the infected and mock plants obtained from each treatment as follows: 
     Direct root infection—Root and basal stem tissues from plants growing under normal or fungal infection conditions were sampled at 6, 24 and 72 hours post infection (hpi) and RNA was extracted as described hereinabove. 
     Identification and Validation of Gene Associated with Fungal Infection 
     In order to discover gene expressed in the examined plant species and plant lines with fungal infection, the present inventors utilized available micro-arrays as described in details hereinbelow for each plant species examined. To define correlations between the levels of RNA expression and fungal resistance, parameters related to plant response to fungal infection were analyzed under normal and infected conditions. From plant identified as encompassing variance in the resistance or susceptibility spectrum, hybrids were selected for further association analysis between fungal infection and gene expression after the plants were challenged with  F. verticillioides  or  F. graminearum  as described hereinabove. 
     Fungal infection was phenotyped as follows: 
     Plants infected via the direct root infection were phenotyped (24 and 72 hpi) for fungal recovery from root and stem tissues by culturing sterilized explants on PDA for 4-5 days and validating the presence of the Fv-GFP strain. For plants infected with Fg, development of necrosis on the roots was monitored along 7 days. 
     Example 2.1: Production of  B. distachyon  Plant Transcriptomes 
     The association of gene expression in  Brachypodium  lines with fungal infection was investigated utilizing a  Brachypodium  oligonucleotide micro-array, produced by Agilent Technologies [chem.agilent.com/Scripts/PDS.asp?lPage=50879]. The array oligonucleotide represents about 27,500  Brachypodium  genes and transcripts. To define correlations between the levels of RNA expression and fungal resistance, parameters related to responses to fungal infection were analyzed in 30 different  Brachypodium  lines under normal and infected conditions as described hereinabove. Among them, 5 lines encompassing susceptibility to  F. graminearum  (designated as “sensitive”, Table 1) were selected for RNA expression analysis after challenge with  F. verticillioides  or  F. graminearum  as described hereinabove. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                   Brachypodium  varieties used for production of transcriptomic 
               
               
                 data and their phenotypic response to  F. graminearum  infection 
               
            
           
           
               
               
               
            
               
                   
                   
                 Response to 
               
               
                   
                 Variety 
                 
                   F. graminearum 
                 
               
               
                   
                   
               
               
                   
                 Bd20-1 
                 Sensitive 
               
               
                   
                 Bd25-1 
                 Sensitive 
               
               
                   
                 PI254868 
                 Sensitive 
               
               
                   
                 Bd9-1 
                 Sensitive 
               
               
                   
                 Bd26-1 
                 Sensitive 
               
               
                   
                   
               
            
           
         
       
     
     Example 2.2: Production of  Setaria italica  Transcriptome for Discovery of Gene Correlating with Resistance to the Fungal Infection 
     The association of gene expression in  Setaria italica  lines with fungal infection was investigated utilizing a  Setaria italica  oligonucleotide micro-array, produced by Agilent Technologies [chem.agilent.com/Scripts/PDS.asp?lPage=50879]. The array oligonucleotide represents about 27,500  Setaria italica  genes and transcripts designed based on data from Public databases (Example 1). To define correlations between the levels of RNA expression and fungal resistance related parameters in responses to fungal infection various plant characteristics of 30 different  Setaria italica  hybrids were analyzed under normal and infected conditions as described hereinabove. Among them, 5 hybrids encompassing medium resistance to  F. graminearum  (designated as “medium resistant”, Table 2) were selected for RNA expression analysis after challenge with  F. verticillioides  or  F. graminearum   
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                   Setaria italica  varieties used for production of transcriptomic 
               
               
                 data and their phenotypic response to  F. graminearum  infection 
               
            
           
           
               
               
               
            
               
                   
                   
                 Response to 
               
               
                   
                 Variety 
                 
                   F. graminearum 
                 
               
               
                   
                   
               
               
                   
                 PI231736 
                 Medium resistant 
               
               
                   
                 PI473596 
                 Medium resistant 
               
               
                   
                 PI253494 
                 Medium resistant 
               
               
                   
                 PI269971 
                 Medium resistant 
               
               
                   
                 Ames27267 
                 Medium resistant 
               
               
                   
                   
               
            
           
         
       
     
     Example 2.3: Production of  Aegilops  Spp. Transcriptome 
     In order to define correlations between the levels of RNA expression with fungal resistance related parameters, responses to fungal infection of 30 different  Aegilops  varieties were analyzed under normal and infected conditions as described hereinabove. Among them, 5 hybrids encompassing resistance to  F. graminearum  (designated resistant”, Table 3) were selected for RNAseq expression analysis (Illumina) after challenge with  F. verticillioides  or  F. graminearum . 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                   Aegilops  varieties used for production of transcriptomic data 
               
               
                 and their phenotypic response to  F. garminearum  infection 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 Response to 
               
               
                   
                 Species 
                 Variety 
                 
                   F. graminearum 
                 
               
               
                   
                   
               
               
                   
                 
                   Aegilops geniculata 
                 
                 Coll3-III 
                 Resistant 
               
               
                   
                 
                   Aegilops geniculata 
                 
                 Coll3-VI 
                 Resistant 
               
               
                   
                 
                   Aegilops tauschii 
                 
                 L84 TQ20 
                 Resistant 
               
               
                   
                 
                   Aegilops tauschii 
                 
                 TQ106 
                 Resistant 
               
               
                   
                 
                   Aegilops sharonensis 
                 
                 6118VI 
                 Resistant 
               
               
                   
                   
               
            
           
         
       
     
     Example 2.4: Production of Maize Transcriptome 
     The association of gene expression in Maize lines with fungal infection was investigated utilizing a Maize oligonucleotide micro-array, produced by Agilent Technologies [chem.agilent.com/Scripts/PDS.asp?lPage=50879]. The array oligonucleotide represents about 60K Maize genes and transcripts designed based on data from Public databases (Example 1). To define correlations between the levels of RNA expression and fungal resistance related parameters responses to fungal infection of various plant characteristics of 30 different Maize hybrids were analyzed under normal and infected conditions as described hereinabove. Among them, 6 hybrids encompassing variance in the resistance spectrum to  F. verticillioides  or  C. graminicola  (designated as “tolerant” and “sensitive”, Table 4) were selected for RNA expression analysis after challenge with  F. verticillioides  or  F. graminearum   
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Maize varieties used for production of transcriptomic data and 
               
               
                 their phenotypic response to  F. verticillioides  infection 
               
            
           
           
               
               
               
            
               
                   
                   
                 Response to 
               
               
                   
                 Variety 
                 
                   F. verticillioides 
                 
               
               
                   
                   
               
               
                   
                 32W86 
                 Tolerant 
               
               
                   
                 Klips 
                 Sensitive 
               
               
                   
                 W182E 
                 Tolerant 
               
               
                   
                 B84 
                 Sensitive 
               
               
                   
                 NC350 
                 Tolerant 
               
               
                   
                 Ky WS4 
                 Sensitive 
               
               
                   
                   
               
            
           
         
       
     
     Example 2.5: Production of Wheat Transcriptome 
     The association of gene expression in wheat lines to fungal infection was investigated utilizing a wheat oligonucleotide micro-array, produced by Agilent Technologies [chem.agilent.com/Scripts/PDS.asp?lPage=50879]. The array oligonucleotide represents about 50,000 wheat genes and transcripts. 
     In order to define correlations between the levels of RNA expression with fungal resistance related parameters, responses to fungal infection of 30 different wheat varieties were analyzed under normal and infected conditions as described hereinabove. Among them, 6 hybrids encompassing variance in the resistance spectrum to  F. verticillioides  (designated as “tolerant” and “sensitive”, Table 5) were selected for RNA expression analysis after challenge with  F. verticillioides  or  F. graminearum . 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Wheat varieties used for production of transcriptomic data and 
               
               
                 their phenotypic response to  F. verticillioides  infection 
               
            
           
           
               
               
               
            
               
                   
                   
                 Response to 
               
               
                   
                 Variety 
                 
                   F. verticillioides 
                 
               
               
                   
                   
               
               
                   
                 Aurora 
                 Sensitive 
               
               
                   
                 Precoce 
                 Tolerant 
               
               
                   
                 Barani 
                 Sensitive 
               
               
                   
                 N46 
                 Tolerant 
               
               
                   
                 Bobwhite 
                 Sensitive 
               
               
                   
                 Thacher 
                 Tolerant 
               
               
                   
                   
               
            
           
         
       
     
     Differential Expression Analysis 
     The analysis was preformed via proprietary differential expression algorithm. 
     The default query parameters used were: &gt;2 fold change, p value&lt;0.01, FDR&lt;0.5 (FDR=false discovery rate). Stringency varied due to specific experimental context. The following queries were performed across species (aggregated through the use of proprietary ortholog determination), germplasm, organs, types of pathogens treated, and time post infection: 
     1. Up regulation upon infection: the gene&#39;s expression level is higher in infected samples than in mock controls (both resistant and susceptible lines are queried). 
     2. Stronger expression induction in resistant lines: the gene&#39;s expression induction is higher in resistant than in susceptible lines upon infection. 
     3. Higher basal expression in resistant lines: the gene&#39;s expression is higher in resistant than in susceptible lines in uninfected samples. 
     No type of query is necessary nor sufficient but overall enrichment of positive indications is considered to identify genes significantly qualifying the above criteria 
     Results 
     The genes identified using the above differential expression analyses and the indications found per gene are described hereinbelow: 
     LFS90 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hours post infection—hpi). 
     Wheat—The wheat gene ortholog was upregulated in the roots and stem in response to Fv at early infection stages (6 and 72 hpi, respectively), and in the inflorescence in response to  Fusarium graminearum  (Fg) at early infection stages (30 and 50 hpi). 
     LFS91 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). 
     Barley—The barley gene ortholog was upregulated at both early and late stages following inflorescence infection with  Fusarium graminearum  (Fg) or challenging assays with the mycotoxin Deoxynivalenol (DON) 1, 2, 3, 4- and 6-days post infection (dpi). 
       Brachypodium —The  Brachypodium  ortholog was upregulated in the roots of several genotypes in response to Fg at early infection stages (48 hpi). 
     Maize—The maize gene ortholog was upregulated in response to  Colletotrichum graminicola  (Cg) at early infection stage (120 hpi). 
       Sorghum —The  sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (6, 24 and 72 hpi). 
     Wheat—The wheat ortholog was upregulated in the inflorescence in response to Fg at early infection stages (50 hpi). QTL—The rice ortholog mapped to a QTL for blast disease resistance. 
     LFS92 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). 
     Wheat—The wheat ortholog was upregulated in the inflorescence in response to  Fusarium graminearum  (Fg) at early infection stages (50 hpi). 
     Maize—The maize ortholog was upregulated in the inflorescence in response to Fv at early infection stages (72 and 96 hpi). In addition, the ortholog was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early infection stages (96 and 120 hpi). 
     QTL—The soybean ortholog mapped to a QTL for fungal disease resistance. 
     LFS93 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). 
     QTL—The maize ortholog mapped to QTLs for  Fusarium  ear rot resistance and for resistance to contamination with the mycotoxin fumonisin. 
     LFS94 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). 
     Wheat—The wheat ortholog was upregulated in the roots of resistant genotypes in response to Fv at early infection stages (1.5 and 2.5 hpi) and in the inflorescence in response to  Fusarium graminearum  (Fg) at early infection stages (30 and 50 hpi) 
     LFS95 
       Brachypodium —The  Brachypodium  ortholog gene was upregulated following spikes inoculation with  Fusarium graminearum  (Fg) (96 hpi). 
     Wheat—The wheat ortholog was upregulated in the roots of resistant genotypes in response to Fv at early infection stages (1.5 and 2.5 hpi) and in the inflorescence in response to  Fusarium graminearum  (Fg) at early infection stages (30 and 50 hpi). 
     Maize—The maize ortholog was upregulated in the inflorescence in response to Fv at early infection stages (96 hpi). In addition, the ortholog was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early infection stages (96 and 120 hpi). 
     LFS96 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv), and in several genotypes also to  Fusarium graminearum  (Fg) at early infection stage (6 and 24 hpi). 
     Maize—The maize ortholog was upregulated in the inflorescence in response to Fv at early infection stages (72 and 96 hpi). In addition, the ortholog was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early infection stages (72, 96 and 120 hpi). 
     QTL—The rice ortholog mapped to a QTL for blast disease resistance. 
     LFS97 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). In addition, an upregulation in several genotypes was observed in the roots in response to  Fusarium graminearum  (Fg) at early infection stages (6 and 24 hpi). 
     Barley—The barley ortholog was upregulated at early and late stages following inflorescence infection with Fg (72, 96 and 144 hpi) or challenging assays with the mycotoxin Deoxynivalenol (DON) (12 and 24 hpi). 
     Wheat—The wheat ortholog was upregulated in the inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
     Maize—The maize ortholog was upregulated in the inflorescence in response to Fv at early infection stage (72 hpi). In addition, the gene was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early infection stage (120 hpi) and was upregulated in the inflorescence following Cg infection (72 hpi). 
       Sorghum —The  sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (2.5 and 6 hpi). 
     QTL—The maize ortholog mapped to a QTL for resistance to the mycotoxin fumonisin contamination. The  Sorghum  ortholog mapped to a QTL for rust resistance. 
     LFS98 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hours post infection—hpi). In addition, an upregulation in several genotypes was observed in the roots, in response to  Fusarium graminearum  (Fg) at early infection stages (24 hpi). 
     Maize—The maize ortholog was upregulated in the roots in response to Fv and Fg at early infection stages (6 hpi and 72 hpi, respectively). In addition, the ortholog was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early infection stage (96 and 120 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (24 hpi). 
     Wheat—The wheat ortholog was upregulated in the roots in response to Fv at late infection stages (10 days post infection—dpi), and in the inflorescence in response to Fg at early infection stages (50 hpi). 
     QTL—The maize ortholog mapped to a QTL for  Fusarium  ear rot resistance. The rice ortholog mapped to a QTL for sheath blight disease resistance. 
     LFS99 
     Barley—The barley ortholog was upregulated at both early and late stages following inflorescence infection with  Fusarium graminearum  (Fg) or challenging assays with the mycotoxin Deoxynivalenol (DON) (1, 2, 3, 4 and 6 dpi). 
     Wheat—The wheat ortholog was upregulated in the roots in response to Fv at early infection stages (6 and 24 hpi). In addition, the ortholog was upregulated in the inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
     LFS100 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hours post infection—hpi). In addition, an upregulation in several genotypes was observed in the roots, in response to  Fusarium graminearum  (Fg) at early infection stages (6 and 24 hpi). 
     Wheat—The wheat ortholog was upregulated in the roots of resistant genotypes in response to Fv at early infection stages (6 and 24 hpi) and in the inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
       Brachypodium —The  Brachypodium  ortholog was upregulated in the roots at early stages of both Fv and Fg infections (6 and 24 hpi). 
     Barley—The barley ortholog was upregulated at both early and late stages following inflorescence infection with Fg or challenging assays with the mycotoxin Deoxynivalenol (DON) (1, 2, 3, 4 and 6 dpi). 
     QTL—The rice ortholog mapped to a QTL for blast disease resistance 
     LFS102 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hours post infection—hpi). 
     Wheat—The wheat ortholog was upregulated in the inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
     Barley—The barley ortholog was upregulated at late stages following inflorescence infection with Fg (3, 4- and 6-days post infection—dpi) or at early stage following challenging assays with the mycotoxin Deoxynivalenol (DON) (2 and 4 dpi). 
     Maize—The maize ortholog was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early and late infection stages (120 hpi) and was upregulated in the inflorescence following Cg infection (4 and 6 dpi). QTL—The maize ortholog mapped to a QTL for fumonisin contamination resistance. 
     LFS104 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). In addition, an upregulation in several genotypes was observed in the roots, in response to  Fusarium graminearum  (Fg) at early infection stages (24 hpi). 
     Wheat—The wheat ortholog was upregulated in the roots in response to Fv at early infection stages (6 and 24 hpi) and in the inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
       Brachypodium —The  Brachypodium  ortholog was moderately upregulated following spikes inoculation with Fg (96 hpi). 
     Barley—The barley ortholog was upregulated at both early and late stages following inflorescence infection with Fg or challenging assays with the mycotoxin Deoxynivalenol (DON) (1, 2, 3, 4- and 6-days post infection—dpi). 
     Maize—The maize ortholog was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early and late infection stages (120 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (6 hpi). 
     QTL—The maize ortholog mapped to QTLs for fumonisin contamination resistance. The rice ortholog mapped to a QTL for blast disease resistance. 
     LFS105 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). 
     Maize—The maize ortholog was upregulated in the inflorescence in response to Fv at early infection stages (72 hpi). In addition, the ortholog was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early infection stage (120 hpi). 
     Wheat—The wheat ortholog was upregulated in the roots in response to Fv at early infection stages (6 and 24 hpi) and in the inflorescence in response to  Fusarium graminearum  (Fg) at early infection stages (30 and 50 hpi). 
     QTL—The rice ortholog mapped to a QTL for blast disease resistance 
     LFS106 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). 
       Brachypodium —The  Brachypodium  ortholog was upregulated in the roots of several genotypes in response to Fv at early infection stage (48 hpi). In addition, the gene was upregulated following spikes inoculation with  Fusarium graminearum  (Fg) (96 hpi). 
     Wheat—The wheat ortholog was upregulated in the inflorescence in response to Fg at early infection stages (50 hpi). 
     Maize—The maize ortholog was upregulated in the inflorescence in response to Fv at early infection stage (72 hpi). In addition, the gene was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early infection stage (120 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (6 and 24 hpi) 
     LFS107 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). In addition, in several genotypes upregulation was observed in the roots in response to  Fusarium graminearum  (Fg) at early infection stages (24 hpi). 
     Foxtail millet ( Setaria italica )—The foxtail millet ortholog was moderately upregulated in the roots in response to Fg at early infection stages (24 and 48 hpi). 
     Wheat—The wheat ortholog was upregulated in the roots in response to Fv at early infection stages (6 hpi) and in the inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
     QTL—The rice ortholog mapped to a QTL for blast disease resistance. 
     LFS109 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi) and upregulated moderately in the roots in response to  Fusarium graminearum  (Fg) at early infection stage (6 and 24 hpi). 
       Brachypodium —The  Brachypodium  ortholog was upregulated in the roots of several genotypes in response to Fg at early infection stages (48 hpi). 
     Maize—The maize ortholog was upregulated in the inflorescence in response to Fv at early infection stages (72 hpi). In addition, the ortholog was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early infection stage (48, 96 and 120 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (6 and 24 hpi). 
     LFS110 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). In addition, an upregulation in several genotypes was observed in the roots, in response to  Fusarium graminearum  (Fg) at early infection stages (24 hpi). 
     Barley—The barley ortholog was upregulated at early infection stages following inflorescence infection with Fg (96 hpi) or challenging assays with the mycotoxin Deoxynivalenol (DON) (1 and 12 hpi). 
     Maize—The maize ortholog was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early infection stages (120 hpi). 
     QTL—The maize ortholog mapped to QTLs for  Fusarium  ear rot resistance. The rice ortholog mapped to a QTL for blast disease resistance. The soybean ortholog mapped to a QTL for fungal disease resistance. 
     LFS111 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). 
     Foxtail millet ( Setaria italica )—The foxtail millet ortholog was moderately upregulated in the roots of several genotypes in response to  Fusarium graminearum  (Fg) at early infection stages (48 hpi). 
       Brachypodium —The  Brachypodium  ortholog was moderately upregulated in the roots of several genotypes in response to Fg at early infection stages (48 hpi). 
     QTL—The maize ortholog mapped to QTLs for resistance to fumonisin contamination. The rice ortholog mapped to a QTL for blast disease resistance. 
     LFS112 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and hpi). 
     Foxtail millet ( Setaria italica )—The foxtail millet ortholog was upregulated in the roots of several genotypes at early infection stages of Fv infection (6 and 24 hpi). 
     Maize—The maize ortholog was upregulated in the inflorescence in response to Fv at early infection stage (72 hpi). In addition, the gene was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early and late infection stages (120 hpi) and was upregulated in the inflorescence following Cg infection (4 and 6 dpi). 
     QTL—The rice ortholog mapped to a QTL for sheath blast disease resistance. The soybean ortholog is mapped to a QTL for a fungal disease resistance 
     LFS113 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). 
     Barley—The barley ortholog was upregulated at both early and late stages following inflorescence infection with  Fusarium graminearum  (Fg) or challenging assays with the mycotoxin Deoxynivalenol (DON) (1, 2, 3, 4 and 6 dpi). 
     Foxtail millet ( Setaria italica )—The foxtail millet ortholog was upregulated in the roots of several genotypes in response to Fg at early infection stages (24 and 48 hpi). 
       Brachypodium —The  Brachypodium  ortholog was upregulated in the roots of several genotypes in response to Fg at early infection stages (48 hpi). 
     Wheat—The wheat ortholog was upregulated in the inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
     QTL—The  Sorghum  ortholog mapped to a QTL for anthracnose resistance. The rice ortholog mapped to a QTL for blast disease resistance. 
     LFS114 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticilloides  (Fv) and  Fusarium graminearum  (Fg) at early infection stage (6 and 24 hpi). 
       Brachypodium —The gene was upregulated in the roots in response to Fg at early infection stage (24 and 48 hpi). 
     Barley—The barley ortholog was upregulated at early stages following inflorescence challenging with the mycotoxin Deoxynivalenol (DON) (1 and 12 hpi). 
     Wheat—The wheat ortholog was upregulated in inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
     Maize—The maize ortholog was upregulated in the roots in response to Fv at early (6 hpi) and late (6 and 14 dpi) infection stages. In addition, the ortholog was upregulated in the inflorescence in response to Fv at early infection stage (72 hpi). The ortholog was also upregulated in the roots in response to Fg at early infection stages (24 and 72 hpi). An upregulation was also observed in the leaves in response to  Colletotrichum graminicola  (Cg) infection (96 and 120 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (6 and 24 hpi) 
     LFS115 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium graminearum  (Fg) at early infection stage (6 and 24 hpi). 
     LFS116 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). 
       Brachypodium —The  Brachypodium  ortholog was upregulated following spikes inoculation with  Fusarium graminearum  (Fg) (96 hpi). 
     Wheat—The wheat ortholog was upregulated in the inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
     QTL—The rice ortholog mapped to a QTL for blast disease resistance. 
     LFS117 
       Aegilops —The gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hours post infection—hpi). 
       Brachypodium —The  Brachypodium  ortholog was upregulated following spikes inoculation with  Fusarium graminearum  (Fg) (96 hpi). 
     Maize—The maize ortholog was upregulated in the roots in response to Fg at early infection stages (72 hpi). 
     QTL—The maize ortholog mapped to a QTL for  Fusarium  ear rot resistance 
     LFS121 
     Foxtail millet ( Setaria italica )—The gene was upregulated in the roots at early stages of  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) infection (6 and 24 hpi). 
       Aegilops —The  Aegilops  ortholog was upregulated in the roots at early infection stages of Fg and Fv infection (6 and 24 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (6 and 24 hpi). 
     Wheat—The wheat ortholog was upregulated in the roots of resistant genotypes in response to Fv at early infection stages (6 hpi) and in the inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
     Maize—The maize ortholog was upregulated in the leaves in response to  Colletotrichum graminicola  (Cg) at early infection stages (120 hpi). 
     QTL—The maize ortholog mapped to a QTL for  Fusarium  ear rot resistance. 
     LFS122 
     Foxtail millet ( Setaria italica )—The gene was upregulated in the roots of several genotypes in response to  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) at early infection stages (6 and 24 hpi). 
       Aegilops —The  Aegilops  ortholog was upregulated in the roots in response to Fv at early infection stage (6 and 24 hpi) 
     LFS123 
     Foxtail millet ( Setaria italica )—The gene was upregulated in the roots at early stages of  Fusarium graminearum  (Fg) infection (24 and 48 hpi). 
     LFS124 
     Foxtail millet ( Setaria italica )—The gene was upregulated in the roots at early infection stages of both  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) infections (6 and 24 hpi). 
       Aegilops —The  Aegilops  ortholog was upregulated in the roots in response to Fv at early infection stage (6 and 24 hpi). In addition, an upregulation in several genotypes was observed in the roots, in response to Fg at early infection stages (24 hpi). 
     Wheat—The wheat ortholog was upregulated in the roots in response to Fv at early infection stages (24 and 72 hpi) and in the inflorescence in response to Fg at early infection stages (50 hpi). 
     Barley—The barley ortholog was upregulated at both early and late stages following inflorescence infection with Fg or challenging assays with the mycotoxin Deoxynivalenol (DON) (1, 2, 3, 4 and 6 dpi). 
     Maize—The maize ortholog was upregulated in the inflorescence in response to Fv at early infection stage (72 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (6 and 24 hpi). 
     QTL—The rice ortholog mapped to a QTL for blast disease resistance. The  Sorghum  ortholog mapped to a QTL for rust resistance. 
     LFS125 
     Foxtail millet ( Setaria italica )—The foxtail millet gene was upregulated in the roots in response to  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) at early infection stages (24 hpi). 
       Aegilops —The  Aegilops  ortholog was upregulated in the roots in response to Fv at early infection stage (6 and 24 hpi). 
     Maize—The maize ortholog was upregulated in the inflorescence of resistant genotype in response to Fv at early infection stages (96 hpi). In addition, the ortholog was upregulated in the leaves of resistant genotype in response to  Colletotrichum graminicola  (Cg) at early infection stage (48 and 120 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (6 hpi). 
     QTL—The maize ortholog mapped to a QTL for fumonisin contamination resistance. 
     LFS126 
     Foxtail millet ( Setaria italica )—The gene was upregulated in the roots at early stages of  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) infection (24 and 48 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stages (72 hpi). 
     QTL—The  Sorghum  ortholog mapped to a QTL for anthracnose resistance. 
     LFS127 
     Foxtail millet ( Setaria italica )—The gene was upregulated in the roots at early stages of  Fusarium graminearum  (Fg) infection (24 and 48—hpi). 
       Brachypodium —The  Brachypodium  ortholog was upregulated in the roots at early stages of Fg and  Fusarium verticillioides  (Fv) infection (48 hpi). 
       Aegilops —The  Aegilops  ortholog was upregulated in the roots of several genotypes at early stages of Fg and Fv infection (24 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to Fv at late infection stages (5 dpi). 
     Maize—The maize ortholog was upregulated in the leaves of a resistant genotype in response to  Colletotrichum graminicola  (Cg) at early infection stage (120 hpi). 
     Wheat—The wheat ortholog was upregulated in the roots in response to Fv at early and late infection stages (6, 24 and hpi, 5 and 10 dpi). QTL—The rice ortholog mapped to a QTL for blast disease resistance. The sorghum ortholog mapped to QTLs for anthracnose resistance and for rust resistance. 
     LFS128 
     Foxtail millet ( Setaria italica )—The gene was upregulated in the roots at early infection stages of  Fusarium graminearum  (Fg) infection (24 and 48 hpi). 
       Aegilops —The  Aegilops  ortholog was moderately upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 hpi). 
       Sorghum —The  Sorghum  ortholog was upregulated in the roots in response to Fv at early infection stages (2.5 hpi) 
     LFS129 
       Aegilops —The  Aegilops  ortholog was upregulated in the roots in response to  Fusarium verticillioides  (Fv) at early infection stage (6 and 24 hpi). 
     Wheat—The wheat ortholog was upregulated in the inflorescence in response to Fg at early infection stages (30 and 50 hpi). 
     Barley—The barley ortholog was upregulated at both early and late stages following inflorescence infection with Fg (72, 96 and 144 hpi). 
       Brachypodium —The  Brachypodium  ortholog was upregulated in the roots of several genotypes in response to Fg at early infection stage (48 hpi). 
     QTL—The rice ortholog mapped to a QTL for sheath blight disease resistance. 
     LFS130 
       Aegilops —Computational evidences indicate up-regulation of the gene mainly at early infection stages of  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) in roots. 
     LFS131 
     Foxtail mille ( Setaria italica )—The gene was upregulated in the roots and stem in response to  Fusarium verticillioides  (Fv) at early infection stages. 
     LFS132 
     Maize—Computational evidences indicate upregulation of the gene in roots of sensitive lines at early infection stages of  Fusarium verticillioides  (Fv). 
     LFS82 
     Maize—Computational evidences indicate upregulation of the gene mainly at early infection stages of  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) in different organs 
     Wheat—The wheat ortholog was upregulated in the roots and stem in response to Fv at early infection stages. 
     LFS83 
     Wheat—Computational evidences indicate upregulation of the gene mainly at early infection stages of  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) in different organs. 
       Aegilops  and Maize—The gene orthologs were upregulated in the roots and stem in response to  Fusarium verticillioides  (Fv) at early infection stages. 
     LFS84 
     Wheat—Computational evidences indicate upregulation of the gene and its  Aegilops  ortholog mainly at early infection stages of  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) in different organs 
     Maize—The gene was up regulated as a result of  Colletotrichum graminicola  (Cg) infection in stem tissue. 
     LFS86 
     Maize—Computational evidences indicate upregulation of the gene and its  Sorghum  ortholog mainly at early infection stages of  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) in different organs. 
     LFS87 
     Maize—Computational evidences indicate upregulation of the gene and its wheat ortholog mainly at early infection stages of  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) in different organs 
     LFS89 
     Wheat—Computational evidences indicate upregulation of the gene at late infection stages of  Fusarium graminearum  (Fg) in heads and its  Aegilops  ortholog mainly at early infection stages of  Fusarium verticillioides  (Fv) and  Fusarium graminearum  (Fg) roots. 
     Maize—Computational evidences indicate a positive correlation of the gene expression to the base diameter, stalk width. 
     The plant genes listed in Table 6 below were identified as candidates to have a major impact on plant resistance to at least one of  Fusarium verticilloides, Fusarium graminearum  and  Colletotrichum graminicola  when expression thereof is modulated in plants. The identified gene name, the plant from which it is derived, and the amino acid and nucleic acid sequences of each gene are summarized in Table 6, hereinbelow. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Genes associated with plant resistance to fungal infection 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 Polyn. 
                 Polyp. 
               
               
                   
                   
                   
                 SEQ ID 
                 SEQ ID 
               
               
                   
                 Gene Name 
                 Organism 
                 NO: 
                 NO: 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 LFS82 
                 
                   Zea mays 
                 
                 22 
                 308 
               
               
                   
                 LFS83 
                 
                   Triticum aestivum 
                 
                 23 
                 309 
               
               
                   
                 LFS84 
                 
                   Triticum aestivum 
                 
                 24 
                 310 
               
               
                   
                 LFS86 
                 
                   Zea mays 
                 
                 25 
                 311 
               
               
                   
                 LFS87 
                 
                   Zea mays 
                 
                 26 
                 312 
               
               
                   
                 LFS89 
                 
                   Triticum aestivum 
                 
                 27 
                 313 
               
               
                   
                 LFS90 
                 
                   Aegilops tauschii 
                 
                 28 
                 314 
               
               
                   
                 LFS91 
                 
                   Aegilops tauschii 
                 
                 29 
                 315 
               
               
                   
                 LFS92 
                 
                   Aegilops tauschii 
                 
                 30 
                 316 
               
               
                   
                 LFS93 
                 
                   Aegilops tauschii 
                 
                 31 
                 317 
               
               
                   
                 LFS94 
                 
                   Aegilops tauschii 
                 
                 32 
                 318 
               
               
                   
                 LFS95 
                 
                   Aegilops tauschii 
                 
                 33 
                 319 
               
               
                   
                 LFS96 
                 
                   Aegilops tauschii 
                 
                 34 
                 320 
               
               
                   
                 LFS97 
                 
                   Aegilops tauschii 
                 
                 35 
                 321 
               
               
                   
                 LFS98 
                 
                   Aegilops tauschii 
                 
                 36 
                 322 
               
               
                   
                 LFS99 
                 
                   Aegilops tauschii 
                 
                 37 
                 323 
               
               
                   
                 LFS100 
                 
                   Aegilops tauschii 
                 
                 38 
                 324 
               
               
                   
                 LFS102 
                 
                   Aegilops tauschii 
                 
                 39 
                 325 
               
               
                   
                 LFS104 
                 
                   Aegilops tauschii 
                 
                 40 
                 326 
               
               
                   
                 LFS105 
                 
                   Aegilops tauschii 
                 
                 302 
                 510 
               
               
                   
                 LFS106 
                 
                   Aegilops tauschii 
                 
                 41 
                 327 
               
               
                   
                 LFS107 
                 
                   Aegilops tauschii 
                 
                 42 
                 328 
               
               
                   
                 LFS109 
                 
                   Aegilops tauschii 
                 
                 43 
                 329 
               
               
                   
                 LFS110 
                 
                   Aegilops tauschii 
                 
                 44 
                 330 
               
               
                   
                 LFS111 
                 
                   Aegilops tauschii 
                 
                 45 
                 331 
               
               
                   
                 LFS112 
                 
                   Aegilops tauschii 
                 
                 303 
                 507 
               
               
                   
                 LFS113 
                 
                   Aegilops tauschii 
                 
                 46 
                 332 
               
               
                   
                 LFS114 
                 
                   Aegilops tauschii 
                 
                 47 
                 333 
               
               
                   
                 LFS115 
                 
                   Aegilops tauschii 
                 
                 48 
                 334 
               
               
                   
                 LFS116 
                 
                   Aegilops tauschii 
                 
                 49 
                 335 
               
               
                   
                 LFS117 
                 
                   Aegilops tauschii 
                 
                 50 
                 336 
               
               
                   
                 LFS121 
                 
                   Setaria italica 
                 
                 51 
                 337 
               
               
                   
                 LFS122 
                 
                   Setaria italica 
                 
                 52 
                 338 
               
               
                   
                 LFS123 
                 
                   Setaria italica 
                 
                 53 
                 339 
               
               
                   
                 LFS124 
                 
                   Setaria italica 
                 
                 54 
                 340 
               
               
                   
                 LFS125 
                 
                   Setaria italica 
                 
                 55 
                 341 
               
               
                   
                 LFS126 
                 
                   Setaria italica 
                 
                 56 
                 342 
               
               
                   
                 LFS127 
                 
                   Setaria italica 
                 
                 57 
                 343 
               
               
                   
                 LFS128 
                 
                   Setaria italica 
                 
                 58 
                 344 
               
               
                   
                 LFS129 
                 
                   Setaria italica 
                 
                 59 
                 345 
               
               
                   
                 LFS130 
                 
                   Aegilops tauschii 
                 
                 60 
                 346 
               
               
                   
                 LFS131 
                 
                   Setaria italica 
                 
                 61 
                 347 
               
               
                   
                 LFS132 
                 
                   Zea mays 
                 
                 62 
                 348 
               
               
                   
                   
               
               
                   
                 “polyn.” = polynucleotide; 
               
               
                   
                 “polyp.” = polypeptide. 
               
            
           
         
       
     
     Example 3: Identification of Homologous Sequences Associated with Fungal Resistance 
     The concepts of orthology and paralogy have recently been applied to functional characterizations and classifications on the scale of whole-genome comparisons. Orthologs and paralogs constitute two major types of homologs: The first evolved from a common ancestor by specialization, and the latter are related by duplication events. It is assumed that paralogs arising from ancient duplication events are likely to have diverged in function while true orthologs are more likely to retain identical function over evolutionary time. 
     To further investigate and identify putative orthologs of the genes identified to affect plant resistance to  Fusarium verticilloides, Fusarium graminearum  or  Colletotrichum graminicola , all sequences were aligned using the BLAST™ (Basic Local Alignment Search Tool). Sequences sufficiently similar were tentatively grouped. These putative orthologs were further organized under a Phylogram—a branching diagram (tree) assumed to be a representation of the evolutionary relationships among the biological taxa. Putative ortholog groups were analyzed as to their agreement with the phylogram and in cases of disagreements these ortholog groups were broken accordingly. 
     Expression data were analyzed and the EST libraries were classified using a fixed vocabulary of custom terms such as developmental stages (e.g., genes showing similar expression profile through development with up-regulation at specific stage, such as at seed germination, time points during seedling growth and at maturity) and/or plant organ (e.g., genes showing similar expression profile across their organs with up-regulation at specific organs such as roots and stems). The annotations from all the ESTs clustered to a gene were analyzed statistically by comparing their frequency in the cluster versus their abundance in the database, allowing the construction of a numeric and graphic expression profile of that gene, which is termed “digital expression”. The rationale of using these two complementary methods with methods of phenotypic association studies of QTLs, SNPs and phenotype expression correlation is based on the assumption that true orthologs are likely to retain identical function over evolutionary time. These methods provide different sets of indications on function similarities between two homologous genes, including similarities in the sequence level—identical amino acids in the protein domains and similarity in expression profiles. 
     The search and identification of homologous genes involves the screening of sequence information available, for example, in public databases such as the DNA Database of Japan (DDBJ), GenBank, and the European Molecular Biology Laboratory Nucleic Acid Sequence Database (EMBL) or versions thereof or the MIPS database. A number of different search algorithms have been developed, including but not limited to the suite of programs referred to as BLAST™ programs. There are five implementations of BLAST™, three designed for nucleotide sequence queries (BLASTN, BLASTX, and TBLASTX) and two designed for protein sequence queries (BLASTP and TBLASTN) (Altschul, S. F et al., (1990) “Basic local alignment search tool.” J. Mol. Biol. 215:403-410). Such methods involve alignment and comparison of sequences. The BLAST™ algorithm calculates percent sequence identity and performs a statistical analysis of the similarity between the two sequences. The software for performing BLAST™ analysis is publicly available through the National Centre for Biotechnology Information. Other such software or algorithms are GAP, BESTFIT, FASTA and TFASTA. GAP uses the algorithm of Needleman and Wunsch (J. Mol. Biol. 48: 443-453, 1970) to find the alignment of two complete sequences that maximizes the number of matches and minimizes the number of gaps. 
     The homologous genes may belong to the same gene family. The analysis of a gene family may be carried out using sequence similarity analysis. To perform this analysis, one may use standard programs for multiple alignments e.g. Clustal W. A neighbor-joining tree of the proteins homologous to the proteins encoded by the genes identified in this invention may be used to provide an overview of structural and ancestral relationships. Sequence identity may be calculated using an alignment program as described above. It is expected that plants other that those examined in the present invention will carry a similar functional gene (ortholog) or a family of similar genes and those genes will provide the same preferred phenotype as the genes presented here. Advantageously, these family members may be useful in the methods of the invention. Example of other plants are included here but not limited to, barley ( Hordeum vulgare ),  Arabidopsis  ( Arabidopsis thaliana ), maize ( Zea mays ), cotton ( Gossypium ), Oilseed rape ( Brassica napus ), Rice ( Oryza sativa ), Sugar cane ( Saccharum officinarum ),  Sorghum  ( Sorghum bicolor ), Soybean ( Glycine max ), Sunflower ( Helianthus annuus ), Tomato ( Lycopersicon esculentum ), and Wheat ( Triticum aestivum ). 
     The above-mentioned analyses for sequence homology can be carried out on a full-length sequence, but may also be based on a comparison of certain regions such as conserved domains. The identification of such domains would also be well within the realm of the person skilled in the art and would involve, for example, a computer readable format of the nucleic acids of the present invention, the use of alignment software programs and the use of publicly available information on protein domains, conserved motifs and boxes. This information is available in the PRODOM (biochem.ucl.ac.uk/bsm/dbbrowser/protocol/prodomqry.html), PIR (pir.Georgetown.edu/) or Pfam (sanger.ac.uk/Software/Pfam/) databases. Sequence analysis programs designed for motif searching may be used for identification of fragments, regions and conserved domains as mentioned above. Preferred computer programs include, but are not limited to, MEME, SIGNALSCAN, and GENESCAN. 
     A person skilled in the art may use the homologous sequences provided herein to find similar sequences in other species and other organisms. Homologues of a protein encompass peptides, oligopeptides, polypeptides, proteins and enzymes having amino acid substitutions, deletions and/or insertions relative to the unmodified protein in question and having similar biological and functional activity as the unmodified protein from which they are derived. To produce such homologues, amino acids of the protein may be replaced by other amino acids having similar properties (conservative changes, such as similar hydrophobicity, hydrophilicity, antigenicity, propensity to form or break a-helical structures or β-sheet structures). Conservative substitution Tables are well known in the art (see for example Creighton T E (1984) Proteins. W.H. Freeman and Company). Homologues of a nucleic acid encompass nucleic acids having nucleotide substitutions, deletions and/or insertions relative to the unmodified nucleic acid in question and having similar biological and functional activity as the unmodified nucleic acid from which they are derived. 
     Polynucleotides and polypeptides with significant homology to the identified genes described in Table 6 (Example 2) were identified from the databases using BLAST™ software with the Blastp and tBlastn algorithms as filters for the first stage, and the needle (EMBOSS package) or Frame+ algorithm alignment for the second stage. Local identity (BLAST™ alignments) was defined with a very permissive cutoff—60% Identity on a span of 60% of the sequences lengths because it is used only as a filter for the global alignment stage. The default filtering of the BLAST™ package was not utilized (by setting the parameter “-F F”). 
     In the second stage, homologs were defined based on a global identity of at least 80% to the core gene polypeptide sequence. Two distinct forms for finding the optimal global alignment for protein or nucleotide sequences were used in this application: 
     1. Between two proteins (following the BLASTP filter): 
     EMBOSS-6.0.1 Needleman-Wunsch algorithm with the following modified parameters: gapopen=8 gapextend=2. The rest of the parameters were unchanged from the default options described hereinabove. 
     2. Between a protein sequence and a nucleotide sequence (following the TBLASTN filter): 
     GenCore 6.0 OneModel application utilizing the Frame+ algorithm with the following parameters: model=frame+_p2n.model mode=qglobal -q=protein. sequence -db=nucleotide.sequence. The rest of the parameters are unchanged from the default options described hereinabove. 
     The query polypeptide sequences were the sequences listed in Table 6 (Example 2). The subject sequences are protein sequences identified in the database based on greater than 80% global identity to the predicted translated sequences of the query nucleotide sequences or to the polypeptide sequences. Homology was calculated as % of identity over the aligned sequences. The identified orthologous and homologous sequences having at least 80% global sequence identity to said sequences are provided in Table 7, below. These homologous genes are expected to increase plant resistance to fungal infection caused by the mentioned pathogens 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Homologues (e.g., orthologues) of genes associated 
               
               
                 with plant resistance to fungal infection 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Polyn. 
                 Hom. to 
                   
                 Polyp. 
                 Hom. to 
                 % 
                   
               
               
                 SEQ ID 
                 Gene 
                   
                 SEQ ID 
                 SEQ ID 
                 glob. 
               
               
                 NO: 
                 Name 
                 Organism 
                 NO: 
                 NO: 
                 Iden. 
                 Algor. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 100 
                 LFS122 
                 
                   Panicum virgatum 
                 
                 — 
                 338 
                 90.76 
                 glotblastn 
               
               
                 101 
                 LFS122 
                 
                   Panicum virgatum 
                 
                 369 
                 338 
                 84.3 
                 globlastp 
               
               
                 102 
                 LFS109 
                 
                   Hordeum vulgare 
                 
                 370 
                 329 
                 81.2 
                 globlastp 
               
               
                 103 
                 LFS86 
                 
                   Sorghum bicolor 
                 
                 371 
                 311 
                 80.3 
                 globlastp 
               
               
                 104 
                 LFS132 
                 
                   Sorghum bicolor 
                 
                 — 
                 348 
                 80.33 
                 glotblastn 
               
               
                 105 
                 LFS107 
                 
                   Triticum aestivum 
                 
                 372 
                 328 
                 90.6 
                 globlastp 
               
               
                 106 
                 LFS116 
                 
                   Triticum aestivum 
                 
                 373 
                 335 
                 99.5 
                 globlastp 
               
               
                 107 
                 LFS116 
                 
                   Hordeum vulgare 
                 
                 374 
                 335 
                 90.9 
                 globlastp 
               
               
                 108 
                 LFS121 
                 
                   Panicum virgatum 
                 
                 375 
                 337 
                 85 
                 globlastp 
               
               
                 109 
                 LFS121 
                 
                   Panicum virgatum 
                 
                 376 
                 337 
                 84.3 
                 globlastp 
               
               
                 110 
                 LFS121 
                 
                   Zea mays 
                 
                 377 
                 337 
                 80.9 
                 globlastp 
               
               
                 111 
                 LFS92 
                 
                   Triticum aestivum 
                 
                 378 
                 316 
                 94.6 
                 globlastp 
               
               
                 112 
                 LFS92 
                 
                   Secale cereale 
                 
                 379 
                 316 
                 87.5 
                 globlastp 
               
               
                 113 
                 LFS95 
                 
                   Aegilops tauschii 
                 
                 380 
                 354 
                 88.7 
                 globlastp 
               
               
                 114 
                 LFS95 
                 
                   Triticum aestivum 
                 
                 381 
                 354 
                 88.3 
                 globlastp 
               
               
                 115 
                 LFS95 
                 
                   Secale cereale 
                 
                 382 
                 354 
                 86 
                 globlastp 
               
               
                 116 
                 LFS95 
                 
                   Secale cereale 
                 
                 383 
                 354 
                 84.8 
                 globlastp 
               
               
                 117 
                 LFS95 
                 
                   Secale cereale 
                 
                 384 
                 354 
                 84.3 
                 globlastp 
               
               
                 118 
                 LFS95 
                 
                   Secale cereale 
                 
                 — 
                 354 
                 83.88 
                 glotblastn 
               
               
                 119 
                 LFS95 
                 
                   Secale cereale 
                 
                 — 
                 354 
                 83.47 
                 glotblastn 
               
               
                 120 
                 LFS95 
                 
                   Triticum aestivum 
                 
                 385 
                 354 
                 83.1 
                 globlastp 
               
               
                 121 
                 LFS95 
                 
                   Triticum aestivum 
                 
                 386 
                 354 
                 80.2 
                 globlastp 
               
               
                 122 
                 LFS106 
                 
                   Secale cereale 
                 
                 387 
                 327 
                 89.2 
                 globlastp 
               
               
                 123 
                 LFS106 
                 
                   Triticum aestivum 
                 
                 388 
                 327 
                 85.5 
                 globlastp 
               
               
                 124 
                 LFS106 
                 
                   Hordeum vulgare 
                 
                 389 
                 327 
                 83.1 
                 globlastp 
               
               
                 125 
                 LFS111 
                 
                   Triticum aestivum 
                 
                 — 
                 331 
                 96.71 
                 glotblastn 
               
               
                 126 
                 LFS111 
                 
                   Hordeum vulgare 
                 
                 390 
                 331 
                 88.6 
                 globlastp 
               
               
                 127 
                 LFS97 
                 
                   Triticum aestivum 
                 
                 391 
                 321 
                 99.8 
                 globlastp 
               
               
                 128 
                 LFS97 
                 
                   Triticum aestivum 
                 
                 392 
                 321 
                 99 
                 globlastp 
               
               
                 129 
                 LFS97 
                 
                   Triticum aestivum 
                 
                 393 
                 321 
                 99 
                 globlastp 
               
               
                 130 
                 LFS97 
                 
                   Hordeum vulgare 
                 
                 394 
                 321 
                 98.1 
                 globlastp 
               
               
                 131 
                 LFS97 
                 
                   Secale cereale 
                 
                 395 
                 321 
                 97.7 
                 globlastp 
               
               
                 132 
                 LFS97 
                 
                   Brachypodium distachyon 
                 
                 396 
                 321 
                 94.7 
                 globlastp 
               
               
                 133 
                 LFS97 
                 
                   Avena sativa 
                 
                 397 
                 321 
                 94.7 
                 globlastp 
               
               
                 134 
                 LFS97 
                 
                   Brachypodium distachyon 
                 
                 398 
                 321 
                 93 
                 globlastp 
               
               
                 135 
                 LFS97 
                 
                   Oryza sativa 
                 
                 399 
                 321 
                 89.1 
                 globlastp 
               
               
                 136 
                 LFS97 
                 
                   Panicum virgatum 
                 
                 400 
                 321 
                 84.4 
                 globlastp 
               
               
                 137 
                 LFS97 
                 
                   Setaria italica 
                 
                 401 
                 321 
                 83.6 
                 globlastp 
               
               
                 138 
                 LFS97 
                 
                   Aegilops tauschii 
                 
                 — 
                 321 
                 82.56 
                 glotblastn 
               
               
                 139 
                 LFS97 
                 
                   Sorghum bicolor 
                 
                 402 
                 321 
                 81.2 
                 globlastp 
               
               
                 140 
                 LFS98 
                 
                   Triticum aestivum 
                 
                 403 
                 357 
                 97.2 
                 globlastp 
               
               
                 141 
                 LFS113 
                 
                   Triticum aestivum 
                 
                 404 
                 332 
                 98.8 
                 globlastp 
               
               
                 142 
                 LFS113 
                 
                   Hordeum vulgare 
                 
                 405 
                 332 
                 97.5 
                 globlastp 
               
               
                 143 
                 LFS113 
                 
                   Brachypodium distachyon 
                 
                 406 
                 332 
                 84.6 
                 globlastp 
               
               
                 144 
                 LFS113 
                 
                   Oryza sativa 
                 
                 407 
                 332 
                 82.7 
                 globlastp 
               
               
                 145 
                 LFS113 
                 
                   Oryza sativa 
                 
                 — 
                 332 
                 81.53 
                 glotblastn 
               
               
                 146 
                 LFS113 
                 
                   Secale cereale 
                 
                 — 
                 363 
                 98.25 
                 glotblastn 
               
               
                 147 
                 LFS113 
                 
                   Secale cereale 
                 
                 408 
                 363 
                 97.9 
                 globlastp 
               
               
                 148 
                 LFS113 
                 
                   Setaria italica 
                 
                 409 
                 363 
                 84 
                 globlastp 
               
               
                 149 
                 LFS113 
                 
                   Sorghum bicolor 
                 
                 410 
                 363 
                 83.1 
                 globlastp 
               
               
                 150 
                 LFS113 
                 
                   Zea mays 
                 
                 411 
                 363 
                 82.2 
                 globlastp 
               
               
                 151 
                 LFS117 
                 
                   Triticum aestivum 
                 
                 — 
                 365 
                 96.87 
                 glotblastn 
               
               
                 152 
                 LFS100 
                 
                   Triticum aestivum 
                 
                 — 
                 359 
                 92.49 
                 glotblastn 
               
               
                 153 
                 LFS100 
                 
                   Aegilops tauschii 
                 
                 — 
                 359 
                 89.67 
                 glotblastn 
               
               
                 154 
                 LFS100 
                 
                   Secale cereale 
                 
                 — 
                 359 
                 80.47 
                 glotblastn 
               
               
                 155 
                 LFS94 
                 
                   Secale cereale 
                 
                 — 
                 318 
                 94.7 
                 glotblastn 
               
               
                 156 
                 LFS94 
                 
                   Secale cereale 
                 
                 — 
                 318 
                 93.32 
                 glotblastn 
               
               
                 157 
                 LFS94 
                 
                   Triticum aestivum 
                 
                 — 
                 318 
                 93.09 
                 glotblastn 
               
               
                 158 
                 LFS94 
                 
                   Hordeum vulgare 
                 
                 412 
                 318 
                 92.2 
                 globlastp 
               
               
                 159 
                 LFS94 
                 
                   Secale cereale 
                 
                 413 
                 318 
                 91.2 
                 globlastp 
               
               
                 160 
                 LFS94 
                 
                   Aegilops tauschii 
                 
                 414 
                 318 
                 90.1 
                 globlastp 
               
               
                 161 
                 LFS94 
                 
                   Brachypodium distachyon 
                 
                 415 
                 318 
                 86.3 
                 globlastp 
               
               
                 162 
                 LFS94 
                 
                   Brachypodium distachyon 
                 
                 416 
                 318 
                 85.8 
                 globlastp 
               
               
                 163 
                 LFS94 
                 
                   Oryza sativa 
                 
                 417 
                 318 
                 83.3 
                 globlastp 
               
               
                 164 
                 LFS94 
                 
                   Secale cereale 
                 
                 418 
                 318 
                 83.1 
                 globlastp 
               
               
                 165 
                 LFS94 
                 
                   Zea mays 
                 
                 419 
                 318 
                 80 
                 globlastp 
               
               
                 166 
                 LFS99 
                 
                   Triticum aestivum 
                 
                 — 
                 358 
                 80 
                 glotblastn 
               
               
                 167 
                 LFS95 
                 
                   Hordeum vulgare 
                 
                 420 
                 319 
                 89 
                 globlastp 
               
               
                 168 
                 LFS95 
                 
                   Aegilops tauschii 
                 
                 421 
                 319 
                 87.9 
                 globlastp 
               
               
                 169 
                 LFS96 
                 
                   Triticum aestivum 
                 
                 422 
                 320 
                 98.1 
                 globlastp 
               
               
                 170 
                 LFS96 
                 
                   Aegilops tauschii 
                 
                 — 
                 320 
                 86.79 
                 glotblastn 
               
               
                 171 
                 LFS130 
                 
                   Triticum aestivum 
                 
                 423 
                 346 
                 98.5 
                 globlastp 
               
               
                 172 
                 LFS130 
                 
                   Hordeum vulgare 
                 
                 424 
                 346 
                 94.8 
                 globlastp 
               
               
                 173 
                 LFS130 
                 
                   Brachypodium distachyon 
                 
                 425 
                 346 
                 83.1 
                 globlastp 
               
               
                 174 
                 LFS82 
                 
                   Panicum virgatum 
                 
                 426 
                 349 
                 94.7 
                 globlastp 
               
               
                 175 
                 LFS82 
                 
                   Panicum virgatum 
                 
                 427 
                 349 
                 93.9 
                 globlastp 
               
               
                 176 
                 LFS82 
                 
                   Oryza sativa 
                 
                 428 
                 349 
                 88.6 
                 globlastp 
               
               
                 177 
                 LFS82 
                 
                   Brachypodium distachyon 
                 
                 429 
                 349 
                 87.1 
                 globlastp 
               
               
                 178 
                 LFS82 
                 
                   Triticum aestivum 
                 
                 430 
                 349 
                 86.4 
                 globlastp 
               
               
                 179 
                 LFS82 
                 
                   Secale cereale 
                 
                 431 
                 349 
                 86.2 
                 globlastp 
               
               
                 180 
                 LFS82 
                 
                   Hordeum vulgare 
                 
                 432 
                 349 
                 86.1 
                 globlastp 
               
               
                 306 
                 LFS112 
                 
                   Triticum aestivum 
                 
                 509 
                 507 
                 99.2 
                 globlastp 
               
               
                 305 
                 LFS112 
                 
                   Hordeum vulgare 
                 
                 508 
                 507 
                 95.6 
                 globlastp 
               
               
                 181 
                 LFS129 
                 
                   Panicum virgatum 
                 
                 433 
                 345 
                 92 
                 globlastp 
               
               
                 182 
                 LFS129 
                 
                   Panicum virgatum 
                 
                 434 
                 345 
                 80.5 
                 globlastp 
               
               
                 183 
                 LFS94 
                 
                   Triticum aestivum 
                 
                 435 
                 353 
                 99.8 
                 globlastp 
               
               
                 184 
                 LFS94 
                 
                   Secale cereale 
                 
                 436 
                 353 
                 96.8 
                 globlastp 
               
               
                 185 
                 LFS94 
                 
                   Secale cereale 
                 
                 437 
                 353 
                 90.8 
                 globlastp 
               
               
                 186 
                 LFS94 
                 
                   Setaria italica 
                 
                 438 
                 353 
                 80.7 
                 globlastp 
               
               
                 187 
                 LFS89 
                 
                   Aegilops tauschii 
                 
                 313 
                 313 
                 100 
                 globlastp 
               
               
                 188 
                 LFS84 
                 
                   Hordeum vulgare 
                 
                 439 
                 310 
                 94.3 
                 globlastp 
               
               
                 189 
                 LFS84 
                 
                   Secale cereale 
                 
                 440 
                 310 
                 90 
                 globlastp 
               
               
                 190 
                 LFS84 
                 
                   Aegilops tauschii 
                 
                 441 
                 310 
                 89.4 
                 globlastp 
               
               
                 191 
                 LFS84 
                 
                   Brachypodium distachyon 
                 
                 442 
                 310 
                 87.4 
                 globlastp 
               
               
                 192 
                 LFS84 
                 
                   Panicum virgatum 
                 
                 443 
                 310 
                 85.8 
                 globlastp 
               
               
                 193 
                 LFS84 
                 
                   Sorghum bicolor 
                 
                 444 
                 310 
                 85.3 
                 globlastp 
               
               
                 194 
                 LFS84 
                 
                   Setaria italica 
                 
                 445 
                 310 
                 84.9 
                 globlastp 
               
               
                 195 
                 LFS84 
                 
                   Zea mays 
                 
                 — 
                 310 
                 83.65 
                 glotblastn 
               
               
                 196 
                 LFS84 
                 
                   Zea mays 
                 
                 446 
                 310 
                 83.2 
                 globlastp 
               
               
                 197 
                 LFS84 
                 
                   Oryza sativa 
                 
                 — 
                 310 
                 80.27 
                 glotblastn 
               
               
                 198 
                 LFS125 
                 
                   Zea mays 
                 
                 447 
                 341 
                 83.8 
                 globlastp 
               
               
                 199 
                 LFS115 
                 
                   Secale cereale 
                 
                 448 
                 334 
                 99.8 
                 globlastp 
               
               
                 200 
                 LFS115 
                 
                   Triticum aestivum 
                 
                 449 
                 334 
                 92.7 
                 globlastp 
               
               
                 201 
                 LFS114 
                 
                   Hordeum vulgare 
                 
                 450 
                 364 
                 92.3 
                 globlastp 
               
               
                 202 
                 LFS114 
                 
                   Secale cereale 
                 
                 451 
                 364 
                 91.4 
                 globlastp 
               
               
                 203 
                 LFS114 
                 
                   Triticum aestivum 
                 
                 452 
                 364 
                 80.8 
                 globlastp 
               
               
                 204 
                 LFS93 
                 
                   Hordeum vulgare 
                 
                 453 
                 317 
                 92.8 
                 globlastp 
               
               
                 205 
                 LFS93 
                 
                   Aegilops tauschii 
                 
                 — 
                 317 
                 89.24 
                 glotblastn 
               
               
                 206 
                 LFS93 
                 
                   Triticum aestivum 
                 
                 — 
                 317 
                 84.39 
                 glotblastn 
               
               
                 207 
                 LFS93 
                 
                   Brachypodium distachyon 
                 
                 — 
                 317 
                 80.38 
                 glotblastn 
               
               
                 208 
                 LFS126 
                 
                   Panicum virgaium 
                 
                 454 
                 342 
                 91.2 
                 globlastp 
               
               
                 209 
                 LFS126 
                 
                   Panicum virgatum 
                 
                 455 
                 342 
                 91.2 
                 globlastp 
               
               
                 210 
                 LFS126 
                 
                   Zea mays 
                 
                 456 
                 342 
                 88 
                 globlastp 
               
               
                 211 
                 LFS126 
                 
                   Sorghum bicolor 
                 
                 457 
                 342 
                 88 
                 globlastp 
               
               
                 212 
                 LFS126 
                 
                   Oryza sativa 
                 
                 458 
                 342 
                 80.5 
                 globlastp 
               
               
                 213 
                 LFS100 
                 
                   Triticum aestivum 
                 
                 459 
                 324 
                 81.9 
                 globlastp 
               
               
                 214 
                 LFS83 
                 
                   Triticum aestivum 
                 
                 460 
                 350 
                 99.7 
                 globlastp 
               
               
                 215 
                 LFS83 
                 
                   Secale cereale 
                 
                 461 
                 350 
                 95.4 
                 globlastp 
               
               
                 216 
                 LFS83 
                 
                   Lolium multiflorum 
                 
                 462 
                 350 
                 87.5 
                 globlastp 
               
               
                 217 
                 LFS83 
                 
                   Lolium multiflorum 
                 
                 463 
                 350 
                 87.5 
                 globlastp 
               
               
                 218 
                 LFS83 
                 
                   Brachypodium distachyon 
                 
                 464 
                 350 
                 83.7 
                 globlastp 
               
               
                 219 
                 LFS83 
                 
                   Setaria italica 
                 
                 465 
                 350 
                 80.5 
                 globlastp 
               
               
                 220 
                 LFS131 
                 
                   Setaria italica 
                 
                 466 
                 347 
                 83.2 
                 globlastp 
               
               
                 221 
                 LFS131 
                 
                   Setaria italica 
                 
                 467 
                 347 
                 81.2 
                 globlastp 
               
               
                 222 
                 LFS131 
                 
                   Sorghum bicolor 
                 
                 468 
                 347 
                 81.2 
                 globlastp 
               
               
                 223 
                 LFS91 
                 
                   Triticum aestivum 
                 
                 315 
                 315 
                 100 
                 globlastp 
               
               
                 224 
                 LFS91 
                 
                   Triticum aestivum 
                 
                 469 
                 315 
                 93.1 
                 globlastp 
               
               
                 225 
                 LFS91 
                 
                   Secale cereale 
                 
                 — 
                 315 
                 91.16 
                 glotblastn 
               
               
                 226 
                 LFS91 
                 
                   Secale cereale 
                 
                 — 
                 315 
                 89.23 
                 glotblastn 
               
               
                 227 
                 LFS91 
                 
                   Secale cereale 
                 
                 470 
                 315 
                 81.4 
                 globlastp 
               
               
                 228 
                 LFS91 
                 
                   Brachypodium distachyon 
                 
                 471 
                 315 
                 80.7 
                 globlastp 
               
               
                 229 
                 LFS83 
                 
                   Secale cereale 
                 
                 472 
                 309 
                 95.6 
                 globlastp 
               
               
                 230 
                 LFS83 
                 
                   Secale cereale 
                 
                 — 
                 309 
                 82.07 
                 glotblastn 
               
               
                 231 
                 LFS83 
                 
                   Aegilops tauschii 
                 
                 473 
                 309 
                 82 
                 globlastp 
               
               
                 232 
                 LFS83 
                 
                   Sorghum bicolor 
                 
                 474 
                 309 
                 80.8 
                 globlastp 
               
               
                 233 
                 LFS83 
                 
                   Triticum aestivum 
                 
                 475 
                 309 
                 80.5 
                 globlastp 
               
               
                 234 
                 LFS102 
                 
                   Triticum aestivum 
                 
                 476 
                 325 
                 96.9 
                 globlastp 
               
               
                 235 
                 LFS102 
                 
                   Hordeum vulgare 
                 
                 477 
                 325 
                 90.2 
                 globlastp 
               
               
                 236 
                 LFS102 
                 
                   Aegilops tauschii 
                 
                 — 
                 325 
                 87.5 
                 glotblastn 
               
               
                 237 
                 LFS104 
                 
                   Hordeum vulgare 
                 
                 478 
                 326 
                 97.8 
                 globlastp 
               
               
                 238 
                 LFS104 
                 
                   Secale cereale 
                 
                 479 
                 326 
                 83.1 
                 globlastp 
               
               
                 239 
                 LFS104 
                 
                   Zea mays 
                 
                 — 
                 326 
                 81.37 
                 glotblastn 
               
               
                 240 
                 LFS99 
                 
                   Avena sativa 
                 
                 — 
                 323 
                 93 
                 glotblastn 
               
               
                 241 
                 LFS99 
                 
                   Triticum aestivum 
                 
                 — 
                 323 
                 87 
                 glotblastn 
               
               
                 242 
                 LFS99 
                 
                   Triticum aestivum 
                 
                 480 
                 323 
                 86 
                 globlastp 
               
               
                 243 
                 LFS99 
                 
                   Triticum aestivum 
                 
                 — 
                 323 
                 82 
                 glotblastn 
               
               
                 244 
                 LFS90 
                 
                   Triticum aestivum 
                 
                 481 
                 314 
                 99.8 
                 globlastp 
               
               
                 245 
                 LFS90 
                 
                   Triticum aestivum 
                 
                 482 
                 314 
                 97.2 
                 globlastp 
               
               
                 246 
                 LFS90 
                 
                   Triticum aestivum 
                 
                 483 
                 314 
                 95.5 
                 globlastp 
               
               
                 247 
                 LFS90 
                 
                   Triticum aestivum 
                 
                 484 
                 314 
                 93.9 
                 globlastp 
               
               
                 248 
                 LFS90 
                 
                   Triticum aestivum 
                 
                 485 
                 314 
                 93.7 
                 globlastp 
               
               
                 249 
                 LFS82 
                 
                   Sorghum bicolor 
                 
                 — 
                 308 
                 97.24 
                 glotblastn 
               
               
                 250 
                 LFS87 
                 
                   Zea mays 
                 
                 486 
                 312 
                 96.9 
                 globlastp 
               
               
                 251 
                 LFS87 
                 
                   Zea mays 
                 
                 486 
                 312 
                 96.9 
                 globlastp 
               
               
                 252 
                 LFS87 
                 
                   Zea mays 
                 
                 — 
                 312 
                 95.92 
                 glotblastn 
               
               
                 253 
                 LFS87 
                 
                   Sorghum bicolor 
                 
                 — 
                 312 
                 95.92 
                 glotblastn 
               
               
                 254 
                 LFS87 
                 
                   Saccharum officinarum 
                 
                 — 
                 312 
                 95.92 
                 glotblastn 
               
               
                 255 
                 LFS87 
                 
                   Cenchrus ciliaris 
                 
                 — 
                 312 
                 94.9 
                 glotblastn 
               
               
                 256 
                 LFS87 
                 
                   Setaria italica 
                 
                 — 
                 312 
                 94.9 
                 glotblastn 
               
               
                 257 
                 LFS87 
                 
                   Zea mays 
                 
                 — 
                 312 
                 94.9 
                 glotblastn 
               
               
                 258 
                 LFS87 
                 
                   Panicum virgatum 
                 
                 — 
                 312 
                 94.9 
                 glotblastn 
               
               
                 259 
                 LFS87 
                 
                   Panicum virgatum 
                 
                 — 
                 312 
                 94.9 
                 glotblastn 
               
               
                 260 
                 LFS87 
                 
                   Zea mays 
                 
                 — 
                 312 
                 93.88 
                 glotblastn 
               
               
                 261 
                 LFS87 
                 
                   Pennisetum glaucum 
                 
                 — 
                 312 
                 93.88 
                 glotblastn 
               
               
                 262 
                 LFS87 
                 
                   Echinochloa colona 
                 
                 — 
                 312 
                 91.84 
                 glotblastn 
               
               
                 263 
                 LFS87 
                 
                   Zea mays 
                 
                 — 
                 312 
                 89.8 
                 glotblastn 
               
               
                 264 
                 LFS87 
                 
                   Oryza sativa 
                 
                 — 
                 312 
                 88.78 
                 glotblastn 
               
               
                 265 
                 LFS87 
                 
                   Oryza sativa 
                 
                 — 
                 312 
                 87.76 
                 glotblastn 
               
               
                 266 
                 LFS87 
                 
                   Hordeum vulgare 
                 
                 — 
                 312 
                 86.73 
                 glotblastn 
               
               
                 267 
                 LFS87 
                 
                   Aegilops tauschii 
                 
                 — 
                 312 
                 85.71 
                 glotblastn 
               
               
                 268 
                 LFS87 
                 
                   Secale cereale 
                 
                 — 
                 312 
                 85.71 
                 glotblastn 
               
               
                 269 
                 LFS87 
                 
                   Secale cereale 
                 
                 — 
                 312 
                 85.71 
                 glotblastn 
               
               
                 270 
                 LFS87 
                 
                   Triticum aestivum 
                 
                 — 
                 312 
                 85.71 
                 glotblastn 
               
               
                 271 
                 LFS87 
                 
                   Triticum aestivum 
                 
                 — 
                 312 
                 85.71 
                 glotblastn 
               
               
                 272 
                 LFS87 
                 
                   Brachypodium distachyon 
                 
                 — 
                 312 
                 84.69 
                 glotblastn 
               
               
                 273 
                 LFS87 
                 
                   Cocos nucifera 
                 
                 — 
                 312 
                 82.65 
                 glotblastn 
               
               
                 274 
                 LFS87 
                 
                   Phalaenopsis aphrodite 
                 
                 — 
                 312 
                 82.65 
                 glotblastn 
               
               
                 275 
                 LFS87 
                 
                   Cocos nucifera 
                 
                 — 
                 312 
                 81.63 
                 glotblastn 
               
               
                 276 
                 LFS87 
                 
                   Cynodon dactylon 
                 
                 — 
                 312 
                 81.63 
                 glotblastn 
               
               
                 277 
                 LFS87 
                 
                   Elaeis guineensis 
                 
                 — 
                 312 
                 81.63 
                 glotblastn 
               
               
                 278 
                 LFS87 
                 
                   Lolium multiflorum 
                 
                 — 
                 312 
                 80.61 
                 glotblastn 
               
               
                 279 
                 LFS87 
                 
                   Avena sativa 
                 
                 — 
                 312 
                 80.61 
                 glotblastn 
               
               
                 280 
                 LFS87 
                 
                   Elaeis guineensis 
                 
                 — 
                 312 
                 80.61 
                 glotblastn 
               
               
                 281 
                 LFS104 
                 
                   Brachypodium distachyon 
                 
                 487 
                 360 
                 92.6 
                 globlastp 
               
               
                 282 
                 LFS104 
                 
                   Setaria italica 
                 
                 488 
                 360 
                 86.7 
                 globlastp 
               
               
                 283 
                 LFS104 
                 
                   Sorghum bicolor 
                 
                 489 
                 360 
                 85.6 
                 globlastp 
               
               
                 284 
                 LFS104 
                 
                   Zea mays 
                 
                 490 
                 360 
                 83.8 
                 globlastp 
               
               
                 285 
                 LFS104 
                 
                   Oryza sativa 
                 
                 491 
                 360 
                 80.9 
                 globlastp 
               
               
                 286 
                 LFS127 
                 
                   Panicum virgatum 
                 
                 492 
                 343 
                 86.2 
                 globlastp 
               
               
                 287 
                 LFS127 
                 
                   Sorghum bicolor 
                 
                 493 
                 343 
                 85.2 
                 globlastp 
               
               
                 288 
                 LFS127 
                 
                   Panicum virgatum 
                 
                 494 
                 343 
                 82.1 
                 globlastp 
               
               
                 289 
                 LFS127 
                 
                   Zea mays 
                 
                 495 
                 343 
                 81.5 
                 globlastp 
               
               
                 290 
                 LFS125 
                 
                   Panicum virgatum 
                 
                 496 
                 367 
                 92.3 
                 globlastp 
               
               
                 291 
                 LFS125 
                 
                   Panicum virgatum 
                 
                 497 
                 367 
                 91.6 
                 globlastp 
               
               
                 292 
                 LFS125 
                 
                   Zea mays 
                 
                 498 
                 367 
                 85.5 
                 globlastp 
               
               
                 293 
                 LFS125 
                 
                   Sorghum bicolor 
                 
                 499 
                 367 
                 84.7 
                 globlastp 
               
               
                 294 
                 LFS125 
                 
                   Oryza sativa 
                 
                 500 
                 367 
                 82.1 
                 globlastp 
               
               
                 295 
                 LFS117 
                 
                   Hordeum vulgare 
                 
                 501 
                 336 
                 94.3 
                 globlastp 
               
               
                 296 
                 LFS117 
                 
                   Secale cereale 
                 
                 — 
                 336 
                 80.97 
                 glotblastn 
               
               
                 297 
                 LFS124 
                 
                   Setaria italica 
                 
                 502 
                 340 
                 90.1 
                 globlastp 
               
               
                 298 
                 LFS124 
                 
                   Sorghum bicolor 
                 
                 503 
                 340 
                 85.7 
                 globlastp 
               
               
                 299 
                 LFS124 
                 
                   Zea mays 
                 
                 504 
                 340 
                 83.3 
                 globlastp 
               
               
                 300 
                 LFS124 
                 
                   Panicum virgatum 
                 
                 505 
                 340 
                 82.3 
                 globlastp 
               
               
                 301 
                 LFS124 
                 
                   Panicum virgatum 
                 
                 506 
                 340 
                 81.8 
                 globlastp 
               
               
                   
               
               
                 “Polyn.” = polynucleotide; “Polyp.” = polypeptide; “Algor.” = algorithm (used for sequence alignment and determination of percent homology); “Hom.”—homology; “iden.”—identity; “glob.”—global. 
               
            
           
         
       
     
     The output of the functional genomics approach described herein is a set of genes highly predicted to improve resistance of a plant to fungal infection by  Fusarium verticilloides, Fusarium graminearum  or  Colletotrichum graminicola  when their expression is modulated (enhanced or downregulated) in said plant. Although each gene is predicted to have its own impact, modifying the mode of expression of more than one gene is expected to provide an additive or synergistic effect on the plant resistance Altering the expression of each gene described herein alone or of a set of genes together increases the overall plant resistance, hence expects to increase agricultural productivity. 
     Example 4: Identification of Domains Comprised within Identified Genes 
     A polypeptide domain refers to a set of conserved amino acids located at specific positions along an alignment of sequences of evolutionarily related proteins. While amino acids at other positions can vary between homologues, amino acids that are highly conserved, and particularly amino acids that are highly conserved at specific positions indicate amino acids that are likely essential in the structure, stability or function of a protein. Identified by their high degree of conservation in aligned sequences of a family of protein homologues, they can be used as identifiers to determine if any polypeptide in question belongs to a previously identified polypeptide family. 
     The Integrated Resource of Protein Families, Domains and Sites (InterPro) database is an integrated interface for the commonly used signature databases for text- and sequence-based searches. The InterPro database combines these databases, which use different methodologies and varying degrees of biological information about well-characterized proteins to derive protein signatures. Collaborating databases include SWISS-PROT, PROSITE, TrEMBL, PRINTS, ProDom and Pfam, Smart and TIGRFAMs. Pfam is a large collection of multiple sequence alignments and hidden Markov models covering many common protein domains and families. Pfam is hosted at the Sanger Institute server in the United Kingdom. 
     Interpro is hosted at the European Bioinformatics Institute in the United Kingdom. InterProScan is the software package that allows sequences (protein and nucleic) to be scanned against InterPro&#39;s signatures. Signatures are predictive models, provided by several different databases that make up the InterPro consortium. 
     InterProScan 5.32-71.0 was used to analyze the polypeptides of the present invention (core and homologues/orthologs) for common domains (Jones P et al., 2014. Bioinformatics, January 2014 (doi:10.1093/bioinformatics/btu031). Briefly, InterProScan is based on scanning methods native to the InterPro member databases. It is distributed with pre-configured method cut-offs recommended by the member database experts and which are believed to report relevant matches. All cut-offs are defined in configuration files of the InterProScan programs. Matches obtained with the fixed cut-off are subject to the following filtering: 
     Pfam filtering: Each Pfam family is represented by two hidden Markov models (HMMs)—ls and fs (full-length and fragment). An HMM model has bit score cut-offs (for each domain match and the total model match) and these are defined in the Gathering threshold (GA) lines of the Pfam database. Initial results are obtained with quite a high common cut-off and then the matches of the signature with a lower score than the family specific cut-offs are dropped. 
     If both the fs and is model for a particular Pfam hits the same region of a sequence, the AM field in the Pfam database is used to determine which model should be chosen—globalfirst(LS); localfirst(FS) or byscore (whichever has the highest e-value). 
     Another type of filtering has been implemented since release of version 4.1. It is based on Clan filtering and nested domains. Further information on Clan filtering can be found in the Pfam website (www.sanger.ac.uk/Pfam) for more information on Clan filtering. 
     TIGRFAMs filtering: Each TIGRFAM HMM model has its own cut-off scores for each domain match and the total model match. These bit score cut-offs are defined in the “trusted cut-offs” (TC) lines of the database. Initial results are obtained with quite a high common cut-off and then the matches (of the signature or some of its domains) with a lower score compared to the family specific cut-offs are dropped. 
     PRINTS filtering: All matches with p-value more than a pre-set minimum value for the signature, are dropped. 
     SMART filtering: The publicly distributed version of InterProScan has a common e-value cut-off corresponding to the reference database size. A more sophisticated scoring model is used on the SMART web server and in the production of pre-calculated InterPro Match data. 
     Exact scoring thresholds for domain assignments are proprietary data. The InterProMatches data production procedure uses these additional smart.thresholds data. It is to be noted that the given cut-offs are e-values (i.e. the number of expected random hits) and therefore are valid only in the context of reference database size and smart.desc data files to filter out results obtained with higher cut-off. 
     It implements the following logic: If the whole sequence E-value of a found match is worse than the ‘cut_low’, the match is dropped. If the domain E-value of a found match is worse than the ‘repeat’ cut-off (where defined) the match is dropped. If a signature is a repeat, the number of significant matches of that signature to a sequence must be greater than the value of ‘repeats’ in order for all matches to be accepted as true (T). 
     If the signature is part of a family (‘family_cut’ is defined), if the domain E-value is worse than the domain cut off (‘cutoff’), the match is dropped. If the signature has “siblings” (because it has a family_cut defined), and they overlap, the preferred sibling is chosen as the true match according to information in the overlaps file. 
     PROSITE patterns CONFIRMation: ScanRegExp is able to verify PROSITE matches using corresponding statistically-significant CONFIRM patterns. The default status of the PROSITE matches is unknown (?) and the true positive (T) status is assigned if the corresponding CONFIRM patterns match as well. The CONFIRM patterns were generated based on the true positive SWISS-PROT PROSITE matches using eMOTIF software with a stringency of 10e-9 P-value. 
     PANTHER filtering: Panther has pre- and post-processing steps. The pre-processing step is intended to speed up the HMM-based searching of the sequence and involves blasting the HMM sequences with the query protein sequence in order to find the most similar models above a given e-value. The resulting HMM hits are then used in the HMM-based search. 
     Panther consists of families and sub-families. When a sequence is found to match a family in the blast run, the sub-families are also scored using HMMER tool (that is, unless there is only 1 sub-family, in which case, the family alone is scored against). 
     Any matches that score below the e-value cut-off are discarded. Any remaining matches are searched to find the HMM with the best score and e-value and the best hit is then reported (including any sub-family hit). 
     GENE3D filtering: Gene3D also employs post-processing of results by using a program called DomainFinder. This program takes the output from searching the Gene3D HMMs against the query sequence and extracts all hits that are more than 10 residues long and have an e-value better than 0.001. If hits overlap at all, the match with the better e-value is chosen. 
     The polypeptides of the invention associated with conferring and/or enhancing the resistance of a plant to at least one fungal pathogen can be characterized by specific amino acid domains. According to certain embodiments, particular domains are conserved within a family of polypeptides as described in Table 8 hereinbelow. Without wishing to be bound by any specific theory or mechanism of action, the conserved domain(s) may indicate common functionally of the polypeptides comprising same. The domains are presented by an identifier (number). Table 9 provides the details of each domain. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Core and homologous polypeptides comprising the same domains 
               
            
           
           
               
               
               
               
            
               
                 Core 
                 Gene 
                   
                 Homologous Polypeptides 
               
               
                 Polypeptide 
                 Name 
                 Characteristic Domains 
                 Comprising the Domains 
               
               
                 (SEQ ID NO) 
                 (Core) 
                 (Domain identifier) 
                 (SEQ ID NOs) 
               
               
                   
               
               
                 308 
                 LFS82 
                 1; 2; 3; 4; 5; 6; 7; 8; 9; 10 in core, 
                 426; 427; 428; 429; 430; 431; 432 
               
               
                   
                   
                 cloned and homologs 
               
               
                 309 
                 LFS83 
                 11; 12; 13; 14; 15; 16 in core, cloned 
                 460; 461; 462; 463; 464; 465; 472; 473; 
               
               
                   
                   
                 and homologs 
                 474; 475 
               
               
                 310 
                 LFS84 
                 2; 17; 18; 5 in core, cloned and 
                 439; 440; 441; 442; 443; 444; 445; 446 
               
               
                   
                   
                 homologs 
               
               
                 311 
                 LFS86 
                 19 in core, cloned and homologs 
                 371 
               
               
                 312 
                 LFS87 
                 20; 21 in core and homologs 
                 486 
               
               
                 313 
                 LFS89 
                 22; 23; 24; 25; 26; 27 in core, cloned 
                 313 
               
               
                   
                   
                 and homologs 
               
               
                 314 
                 LFS90 
                 28; 29; 30 in core, cloned and 
                 481; 482; 483; 484; 485 
               
               
                   
                   
                 homologs 
               
               
                 315 
                 LFS91 
                 31; 32 in core, cloned and 
                 315; 469; 470; 471 
               
               
                   
                   
                 homologs 
               
               
                 316 
                 LFS92 
                 23; 24; 33 in core, cloned and 
                 378; 379 
               
               
                   
                   
                 homologs 
               
               
                 317 
                 LFS93 
                 34 in core, cloned and homologs 
                 453 
               
               
                 318 
                 LFS94 
                 35; 36 in core, cloned and 
                 412; 413; 414; 415; 416; 417; 418; 419; 
               
               
                   
                   
                 homologs 
                 435; 436; 437; 438 
               
               
                 319 
                 LFS95 
                 37 in core, cloned and homologs 
                 380; 381; 382; 383; 384; 385; 386; 420; 
               
               
                   
                   
                   
                 421 
               
               
                 320 
                 LFS96 
                 38; 39 in core, cloned and 
                 422 
               
               
                   
                   
                 homologs 
               
               
                 321 
                 LFS97 
                 40; 41; 42; 43 in core, cloned and 
                 391; 392; 393; 394; 395; 396; 397; 398; 
               
               
                   
                   
                 homologs 
                 399; 400; 401; 402 
               
               
                 322 
                 LFS98 
                 44; 45; 32; 46 in core, cloned and 
                 403 
               
               
                   
                   
                 homologs 
               
               
                 323 
                 LFS99 
                 no domains in core 
               
               
                 324 
                 LFS100 
                 47; 48 in core, cloned and 
                 459 
               
               
                   
                   
                 homologs 
               
               
                 325 
                 LFS102 
                 49; 50 in core, cloned and 
                 476; 477 
               
               
                   
                   
                 homologs 
               
               
                 326 
                 LFS104 
                 51; 52; 53; 54; 55; 56; 57 in core, 
                 478; 479; 487; 488; 489; 490; 491 
               
               
                   
                   
                 cloned and homologs 
               
               
                 327 
                 LFS106 
                 no domains in core 
               
               
                 328 
                 LFS107 
                 35; 36 in core, cloned and 
                 372 
               
               
                   
                   
                 homologs 
               
               
                 329 
                 LFS109 
                 58; 59; 60; 21; 61; 20; 62 in core, 
                 370 
               
               
                   
                   
                 cloned and homologs 
               
               
                 330 
                 LFS110 
                 63 in core and cloned 
               
               
                 331 
                 LFS111 
                 34 in core, cloned and homologs 
                 390 
               
               
                 332 
                 LFS113 
                 65; 66; 67 in core, cloned and 
                 404; 405; 406; 407; 408; 409; 410; 411 
               
               
                   
                   
                 homologs 
               
               
                 333 
                 LFS114 
                 68; 69 in core, cloned and 
                 450; 451; 452 
               
               
                   
                   
                 homologs 
               
               
                 334 
                 LFS115 
                 1; 2; 3; 4; 5; 70; 9; 71; 10 in core, 
                 448; 449 
               
               
                   
                   
                 cloned and homologs 
               
               
                 335 
                 LFS116 
                 20; 72; 21 in core, cloned and 
                 373; 374 
               
               
                   
                   
                 homologs 
               
               
                 336 
                 LFS117 
                 73; 74; 75 in core, cloned and 
                 501 
               
               
                   
                   
                 homologs 
               
               
                 337 
                 LFS121 
                 76; 77; 78 in core, cloned and 
                 375; 376; 377 
               
               
                   
                   
                 homologs 
               
               
                 338 
                 LFS122 
                 79 in core, cloned and homologs 
                 369 
               
               
                 339 
                 LFS123 
                 80; 81; 82; 83; 84 in core and cloned 
               
               
                 340 
                 LFS124 
                 85; 86; 87; 88 in core, cloned and 
                 502; 503; 504; 505; 506 
               
               
                   
                   
                 homologs 
               
               
                 341 
                 LFS125 
                 89 in core, cloned and homologs 
                 447; 496; 497; 498; 499; 500 
               
               
                 342 
                 LFS126 
                 62; 59; 60 in core, cloned and 
                 454; 455; 456; 457; 458 
               
               
                   
                   
                 homologs 
               
               
                 343 
                 LFS127 
                 90; 91; 92; 93; 94 in core, cloned and 
                 492; 493; 494; 495 
               
               
                   
                   
                 homologs 
               
               
                 344 
                 LFS128 
                 95; 96; 97; 98 in core and cloned 
               
               
                 345 
                 LFS129 
                 99; 100; 101; 48; 102; 103; 104; 105; 
                 433; 434 
               
               
                   
                   
                 106; 107 in core, cloned and 
               
               
                   
                   
                 homologs 
               
               
                 346 
                 LFS130 
                 108 in core and homologs 
                 423; 424; 425 
               
               
                 347 
                 LFS131 
                 109; 110; 111; 112 in core and 
                 466; 467; 468 
               
               
                   
                   
                 homologs 
               
               
                 348 
                 LFS132 
                 35; 36 in core 
               
               
                 507 
                 LFS112 
                 64 in core, cloned and homologs 
                 508; 509 
               
               
                 510 
                 LFS105 
                 no domains in core 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Details of identified domains 
               
            
           
           
               
               
               
               
            
               
                 Domain 
                 InterPro 
                   
                   
               
               
                 Identifier 
                 No. 
                 Accession No. 
                 Description 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 1 
                 IPR023298 
                 SSF81665 
                 P-type ATPase, transmembrane domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 2 
                 IPR036412 
                 SSF56784 
                 HAD-like superfamily 
               
               
                 3 
                 IPR023299 
                 G3DSA:3.40.1110.10 
                 P-type ATPase, cytoplasmic domain N 
               
               
                 4 
                 IPR008250 
                 SSF81653 
                 P-type ATPase, A domain superfamily 
               
               
                 5 
                 IPR023214 
                 G3DSA:3.40.50.1000 
                 HAD superfamily 
               
               
                 6 
                 IPR032631 
                 PF16209 
                 Phospholipid-translocating ATPase N-terminal P- 
               
               
                   
                   
                   
                 type ATPase, N-terminal 
               
               
                 7 
                 IPR032630 
                 PF16212 
                 Phospholipid-translocating P-type ATPase C- 
               
               
                   
                   
                   
                 terminal P-type ATPase, C-terminal 
               
               
                 8 
                 IPR006539 
                 TIGR01652 
                 ATPase-Plipid: phospholipid-translocating P-type 
               
               
                   
                   
                   
                 ATPase, flippase P-type ATPase, subfamily IV 
               
               
                 9 
                 IPR001757 
                 TIGR01494 
                 ATPase_P-type: HAD ATPase, P-type, family IC 
               
               
                   
                   
                   
                 P-type ATPase 
               
               
                 10 
                 IPR018303 
                 PS00154 
                 E1-E2 ATPases phosphorylation site. P-type 
               
               
                   
                   
                   
                 ATPase, phosphorylation site 
               
               
                 11 
                 IPR000909 
                 PF00388 
                 Phosphatidylinositol-specific phospholipase C, X 
               
               
                   
                   
                   
                 domain Phosphatidylinositol-specific 
               
               
                   
                   
                   
                 phospholipase C, X domain 
               
               
                 12 
                 IPR017946 
                 G3DSA:3.20.20.190 
                 PLC-like phosphodiesterase, TIM beta/alpha- 
               
               
                   
                   
                   
                 barrel domain superfamily 
               
               
                 13 
                 IPR001192 
                 PR00390 
                 Phospholipase C signature Phosphoinositide 
               
               
                   
                   
                   
                 phospholipase C family 
               
               
                 14 
                 IPR035892 
                 G3DSA:2.60.40.150 
                 C2 domain superfamily 
               
               
                 15 
                 IPR000008 
                 SM00239 
                 C2 domain 
               
               
                 16 
                 IPR001711 
                 PF00387 
                 Phosphatidylinositol-specific phospholipase C, Y 
               
               
                   
                   
                   
                 domain Phospholipase C, phosphatidylinositol- 
               
               
                   
                   
                   
                 specific, Y domain 
               
               
                 17 
                 IPR006379 
                 TIGR01484 
                 HAD-SF-IIB: HAD hydrolase, family IIB HAD- 
               
               
                   
                   
                   
                 superfamily hydrolase, subfamily IIB 
               
               
                 18 
                 IPR003337 
                 PF02358 
                 Trehalose-phosphatase 
               
               
                 19 
                 IPR008889 
                 PF05678 
                 VQ motif 
               
               
                 20 
                 IPR000719 
                 PS50011 
                 Protein kinase domain profile. Protein kinase 
               
               
                   
                   
                   
                 domain 
               
               
                 21 
                 IPR011009 
                 SSF56112 
                 Protein kinase-like domain superfamily 
               
               
                 22 
                 IPR012967 
                 PF08100 
                 Dimerisation domain Plant methyltransferase 
               
               
                 23 
                 IPR036390 
                 SSF46785 
                 Winged helix DNA-binding domain superfamily 
               
               
                 24 
                 IPR036388 
                 G3DSA:1.10.10.10 
                 Winged helix-like DNA-binding domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 25 
                 IPR029063 
                 SSF53335 
                 S-adenosyl-L-methionine-dependent 
               
               
                   
                   
                   
                 methyltransferase 
               
               
                 26 
                 IPR001077 
                 PF00891 
                 O-methyltransferase domain 
               
               
                 27 
                 IPR016461 
                 PIRSF005739 
                 O-methyltransferase COMT-type 
               
               
                 28 
                 IPR008998 
                 PF07468 
                 Agglutinin domain 
               
               
                 29 
                 IPR036242 
                 SSF50382 
                 Agglutinin domain superfamily 
               
               
                 30 
                 IPR005830 
                 PF01117 
                 Aerolysin toxin Aerolysin 
               
               
                 31 
                 IPR001509 
                 PF01370 
                 NAD dependent epimerase/dehydratase family 
               
               
                 32 
                 IPR036291 
                 SSF51735 
                 NAD(P)-binding domain superfamily 
               
               
                 33 
                 IPR000232 
                 PS00434 
                 HSF-type DNA-binding domain signature. Heat 
               
               
                   
                   
                   
                 shock factor (HSF)-type, DNA-binding 
               
               
                 34 
                 IPR005202 
                 PS50985 
                 GRAS family profile. Transcription factor GRAS 
               
               
                 35 
                 IPR003480 
                 PF02458 
                 Transferase family Transferase 
               
               
                 36 
                 IPR023213 
                 G3DSA:3.30.559.10 
                 Chloramphenicol acetyltransferase-like domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 37 
                 IPR007770 
                 PF05078 
                 Protein of unknown function (DUF679) Protein 
               
               
                   
                   
                   
                 DMP 
               
               
                 38 
                 IPR035995 
                 SSF57247 
                 Bowman-Birk type proteinase inhibitor 
               
               
                 39 
                 IPR000877 
                 cd00023 
                 BBI Proteinase inhibitor I12, Bowman-Birk 
               
               
                 40 
                 IPR003593 
                 SM00382 
                 AAA+ ATPase domain 
               
               
                 41 
                 IPR003959 
                 PF00004 
                 ATPase family associated with various cellular 
               
               
                   
                   
                   
                 activities (AAA) ATPase, AAA-type, core 
               
               
                 42 
                 IPR025753 
                 PF14363 
                 Domain associated at C-terminal with AAA 
               
               
                   
                   
                   
                 AAA-type ATPase, N-terminal domain 
               
               
                 43 
                 IPR027417 
                 SSF52540 
                 P-loop containing nucleoside triphosphate 
               
               
                   
                   
                   
                 hydrolase 
               
               
                 44 
                 IPR011032 
                 SSF50129 
                 GroES-like superfamily 
               
               
                 45 
                 IPR020843 
                 SM00829 
                 Polyketide synthase, enoylreductase domain 
               
               
                 46 
                 IPR013149 
                 PF00107 
                 Zinc-binding dehydrogenase Alcohol 
               
               
                   
                   
                   
                 dehydrogenase, C-terminal 
               
               
                 47 
                 IPR003245 
                 PS51485 
                 Phytocyanin domain profile. Phytocyanin domain 
               
               
                 48 
                 IPR008972 
                 SSF49503 
                 Cupredoxin 
               
               
                 49 
                 IPR035897 
                 SSF52200 
                 Toll/interleukin-1 receptor homology (TIR) 
               
               
                   
                   
                   
                 domain superfamily 
               
               
                 50 
                 IPR000157 
                 PF01582 
                 TIR domain Toll/interleukin-1 receptor homology 
               
               
                   
                   
                   
                 (TIR) domain 
               
               
                 51 
                 IPR020833 
                 PS00711 
                 Lipoxygenases iron-binding region signature 1. 
               
               
                   
                   
                   
                 Lipoxygenase, iron binding site 
               
               
                 52 
                 IPR036392 
                 SSF49723 
                 PLAT/LH2 domain superfamily 
               
               
                 53 
                 IPR036226 
                 SSF48484 
                 Lipoxigenase, C-terminal domain superfamily 
               
               
                 54 
                 IPR013819 
                 PR00087 
                 Lipoxygenase signature Lipoxygenase, C-terminal 
               
               
                 55 
                 IPR027433 
                 G3DSA:4.10.372.10 
                 Lipoxygenase, domain 3 
               
               
                 56 
                 IPR020834 
                 PS00081 
                 Lipoxygenases iron-binding region signature 2. 
               
               
                   
                   
                   
                 Lipoxygenase, conserved site 
               
               
                 57 
                 IPR001246 
                 PR00468 
                 Plant lipoxygenase signature Lipoxygenase, plant 
               
               
                 58 
                 IPR017441 
                 PS00107 
                 Protein kinases ATP-binding region signature. 
               
               
                   
                   
                   
                 Protein kinase, ATP binding site 
               
               
                 59 
                 IPR001611 
                 PS51450 
                 Leucine-rich repeat profile. Leucine-rich repeat 
               
               
                 60 
                 IPR032675 
                 G3DSA:3.80.10.10 
                 Leucine-rich repeat domain superfamily 
               
               
                 61 
                 IPR008271 
                 PS00108 
                 Serine/Threonine protein kinases active-site 
               
               
                   
                   
                   
                 signature. 
               
               
                 62 
                 IPR003591 
                 SM00369 
                 Leucine-rich repeat, typical subtype 
               
               
                 63 
                 IPR015425 
                 PS51444 
                 Formin homology-2 (FH2) domain profile. 
               
               
                   
                   
                   
                 Formin, FH2 domain 
               
               
                 64 
                 IPR020864 
                 SM00457 
                 Membrane attack complex component/perforin 
               
               
                   
                   
                   
                 (MACPF) domain 
               
               
                 65 
                 IPR036259 
                 SSF103473 
                 MFS transporter superfamily 
               
               
                 66 
                 IPR020846 
                 cd06174 
                 MFS Major facilitator superfamily domain 
               
               
                 67 
                 IPR000109 
                 PF00854 
                 POT family Proton-dependent oligopeptide 
               
               
                   
                   
                   
                 transporter family 
               
               
                 68 
                 IPR029058 
                 SSF53474 
                 Alpha/Beta hydrolase fold 
               
               
                 69 
                 IPR013094 
                 PF07859 
                 alpha/beta hydrolase fold Alpha/beta hydrolase 
               
               
                   
                   
                   
                 fold-3 
               
               
                 70 
                 IPR004014 
                 PF00690 
                 Cation transporter/ATPase, N-terminus Cation- 
               
               
                   
                   
                   
                 transporting P-type ATPase, N-terminal 
               
               
                 71 
                 IPR006534 
                 TIGR01647 
                 ATPase-IIIA_H: plasma-membrane proton-efflux 
               
               
                   
                   
                   
                 P-type ATPase P-type ATPase, subfamily IIIA 
               
               
                 72 
                 IPR001245 
                 PF07714 
                 Protein tyrosine kinase Serine-threonine/tyrosine- 
               
               
                   
                   
                   
                 protein kinase, catalytic domain 
               
               
                 73 
                 IPR036955 
                 G3DSA:3.30.730.10 
                 AP2/ERF domain superfamily 
               
               
                 74 
                 IPR016177 
                 SSF54171 
                 DNA-binding domain superfamily 
               
               
                 75 
                 IPR001471 
                 PR00367 
                 Ethylene responsive element binding protein 
               
               
                   
                   
                   
                 signature AP2/ERF domain 
               
               
                 76 
                 IPR001005 
                 cd00167 
                 SANT SANT/Myb domain 
               
               
                 77 
                 IPR009057 
                 SSF46689 
                 Homeobox-like domain superfamily 
               
               
                 78 
                 IPR017930 
                 PS51294 
                 Myb-type HTH DNA-binding domain profile. 
               
               
                   
                   
                   
                 Myb domain 
               
               
                 79 
                 IPR025886 
                 PF14299 
                 Phloem protein 2 Phloem protein 2-like 
               
               
                 80 
                 IPR005630 
                 PF03936 
                 Terpene synthase family, metal binding domain 
               
               
                   
                   
                   
                 Terpene synthase, metal-binding domain 
               
               
                 81 
                 IPR036965 
                 G3DSA:1.50.10.130 
                 Terpene synthase, N-terminal domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 82 
                 IPR008949 
                 G3DSA:1.10.600.10 
                 Isoprenoid synthase domain superfamily 
               
               
                 83 
                 IPR008930 
                 SSF48239 
                 Terpenoid cyclases/protein prenyltransferase 
               
               
                   
                   
                   
                 alpha-alpha toroid 
               
               
                 84 
                 IPR001906 
                 PF01397 
                 Terpene synthase, N-terminal domain 
               
               
                 85 
                 IPR016039 
                 SSF53901 
                 Thiolase-like 
               
               
                 86 
                 IPR001099 
                 PF00195 
                 Chalcone and stilbene synthases, N-terminal 
               
               
                   
                   
                   
                 domain Chalcone/stilbene synthase, N-terminal 
               
               
                 87 
                 IPR011141 
                 PIRSF000451 
                 Polyketide synthase, type III 
               
               
                 88 
                 IPR012328 
                 PF02797 
                 Chalcone and stilbene synthases, C-terminal 
               
               
                   
                   
                   
                 domain Chalcone/stilbene synthase, C-terminal 
               
               
                 89 
                 IPR002123 
                 SM00563 
                 Phospholipid/glycerol acyltransferase 
               
               
                 90 
                 IPR011051 
                 SSF51182 
                 RmlC-like cupin domain superfamily 
               
               
                 91 
                 IPR006045 
                 SM00835 
                 Cupin 1 
               
               
                 92 
                 IPR014710 
                 G3DSA:2.60.120.10 
                 RmlC-like jelly roll fold 
               
               
                 93 
                 IPR001929 
                 PR00325 
                 Germin signature Germin 
               
               
                 94 
                 IPR019780 
                 PS00725 
                 Germin family signature. Germin, manganese 
               
               
                   
                   
                   
                 binding site 
               
               
                 95 
                 IPR026961 
                 PF13962 
                 Domain of unknown function PGG domain 
               
               
                 96 
                 IPR036770 
                 G3DSA:1.25.40.20 
                 Ankyrin repeat-containing domain superfamily 
               
               
                 97 
                 IPR020683 
                 cd00204 
                 ANK Ankyrin repeat-containing domain 
               
               
                 98 
                 IPR002110 
                 PS50088 
                 Ankyrin repeat profile. Ankyrin repeat 
               
               
                 99 
                 IPR034289 
                 cd13897 
                 CuRO_3_LCC_plant Laccase, third cupredoxin 
               
               
                   
                   
                   
                 domain 
               
               
                 100 
                 IPR011706 
                 PF07731 
                 Multicopper oxidase Multicopper oxidase, type 2 
               
               
                 101 
                 IPR011707 
                 PF07732 
                 Multicopper oxidase Multicopper oxidase, type 3 
               
               
                 102 
                 IPR033138 
                 PS00079 
                 Multicopper oxidases signature 1. Multicopper 
               
               
                   
                   
                   
                 oxidases, conserved site 
               
               
                 103 
                 IPR002355 
                 PS00080 
                 Multicopper oxidases signature 2. Multicopper 
               
               
                   
                   
                   
                 oxidase, copper-binding site 
               
               
                 104 
                 IPR001117 
                 PF00394 
                 Multicopper oxidase Multicopper oxidase, type 1 
               
               
                 105 
                 IPR017761 
                 TIGR03389 
                 laccase: laccase 
               
               
                 106 
                 IPR034285 
                 cd13875 
                 CuRO_2_LCC_plant Laccase, second cupredoxin 
               
               
                   
                   
                   
                 domain 
               
               
                 107 
                 IPR034288 
                 cd13849 
                 CuRO_1_LCC_plant Laccase, first cupredoxin 
               
               
                   
                   
                   
                 domain 
               
               
                 108 
                 IPR004326 
                 PF03094 
                 Mlo family Mlo-related protein 
               
               
                 109 
                 IPR036396 
                 G3DSA:1.10.630.10 
                 Cytochrome P450 superfamily 
               
               
                 110 
                 IPR001128 
                 PF00067 
                 Cytochrome P450 Cytochrome P450 
               
               
                 111 
                 IPR017972 
                 PS00086 
                 Cytochrome P450 cysteine heme-iron ligand 
               
               
                   
                   
                   
                 signature. Cytochrome P450, conserved site 
               
               
                 112 
                 IPR002401 
                 PR00463 
                 E-class P450 group I signature Cytochrome P450, 
               
               
                   
                   
                   
                 E-class, group I 
               
               
                   
               
            
           
         
       
     
     Example 5: Gene Cloning and Generation of Binary Vectors for Expression in Plants 
     To validate the role of genes identified hereinabove in increasing resistance to fungal infection, selected genes were over-expressed in plants, as follows. 
     Cloning Strategy 
     Selected genes from those presented in Examples 1-4 hereinabove were cloned into binary vectors for the generation of transgenic plants. In addition, to examine the effect of gene stacking, two pairs of genes were also cloned. For cloning, the full-length open reading frames (ORFs) were identified. EST clusters and in some cases mRNA sequences were analyzed to identify the entire open reading frame by comparing the results of several translation algorithms to known proteins from other plant species. 
     In order to clone the full-length cDNAs, reverse transcription (RT) followed by polymerase chain reaction (PCR; RT-PCR) was performed on total RNA extracted from roots or shoots challenged by the relevant pathogen. Total RNA extraction, production of cDNA and PCR amplification was performed using standard protocols described elsewhere (Sambrook J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, New York) which are well known to those skilled in the art. PCR products were purified using PCR purification kit (Qiagen). 
     Typically, 2 sets of primers were prepared for the amplification of each gene, via nested PCR (if required). Both sets of primers were used for amplification on a cDNA. In case no product was obtained, a nested PCR reaction was performed. Nested PCR was performed by amplification of the gene using external primers and then using the produced PCR product as a template for a second PCR reaction, where the internal set of primers was used. Alternatively, one or two of the internal primers were used for gene amplification, both in the first and the second PCR reactions (meaning only 2-3 primers are designed for a gene). To facilitate further cloning of the cDNAs, an 8-12 base pairs (bp) extension was added to the 5′ of each internal primer. The primer extension includes an endonuclease restriction site. The restriction sites were selected using two parameters: (a) the restriction site does not exist in the cDNA sequence; and (b) the restriction sites in the forward and reverse primers were designed such that the digested cDNA was inserted in the sense direction into the binary vector utilized for transformation. 
     PCR products were digested with the restriction endonucleases (New England BioLabs Inc.) according to the sites designed in the primers. Each digested/undigested PCR product was inserted into a high copy vector pUC19 (New England BioLabs Inc.), or into plasmids originating from this vector. In some cases, the undigested PCR product was inserted into pCR-Blunt II-TOPO (Invitrogen) or into pJET1.2 (CloneJET PCR Cloning Kit, Thermo Scientific) or directly into the binary vector. The digested/undigested products and the linearized plasmid vector were ligated using T4 DNA ligase enzyme (Roche, Switzerland or other manufacturers). In cases where pCR-Blunt II-TOPO is used no T4 ligase was needed. 
     Sequencing of the inserted genes was performed using the ABI 377 sequencer (Applied Biosystems). In some cases, after confirming the sequences of the cloned genes, the cloned cDNA was introduced into pQ6sVN plasmid. 
     Several DNA sequences of the selected genes were synthesized by GeneArt™ (Life Technologies, Grand Island, N.Y., USA). Synthetic DNA was designed in silico. Suitable restriction enzyme sites were added to the cloned sequences at the 5′ end and at the 3′ end to enable later cloning into the desired binary vector. 
     Binary vectors—The pPI plasmid vector was constructed by inserting a synthetic poly-(A) signal sequence, originating from pGL3 basic plasmid vector (Promega, GenBank Accession No. U47295; nucleotides 4658-4811) into the HindIII restriction site of the binary vector pBI101.3 (Clontech, GenBank Accession No. U12640). pGI is similar to pPI, but the original gene in the backbone is GUS-Intron and not GUS. 
     The modified pGI vector (e.g., pQFN, pQFNc, pQFNd, pQYN_6669, pQNa_RP, pQFYN, pQXNc or pQ6sVN ( FIG. 1 ) is a modified version of the pGI vector in which the cassette is inverted between the left and right borders so the gene and its corresponding promoter are close to the right border and the hygromycin-resistance conferring gene is close to the left border. 
     In case of  Brachypodium  transformation, after confirming the sequences of the cloned genes, the cloned cDNAs were introduced into pQ6sVN ( FIG. 1 ) containing 35S promoter (SEQ ID NO:37) and the NOS terminator (SEQ ID NO:36) via digestion with appropriate restriction endonucleases. The genes were cloned downstream to the 35S promoter and upstream to the NOS terminator. In the pQ6sVN vector the Hygromycin resistance gene cassette and the Bar_GA resistance gene cassette replaced the NPTII resistance gene cassette. pQ6sVN contains the 35S promoter (SEQ ID NO:37). Bar_GA resistance gene (SEQ ID NO: 39) is an optimized sequence of the BAR gene for expression in Brachypodium plants (ordered from GeneArt™). 
     BAR gene coding sequence is provided in GenBank Accession No. JQ293091.1 (SEQ ID NO:38); further description is provided in Akama K, et al. “Efficient  Agrobacterium -mediated transformation of  Arabidopsis thaliana  using the bar gene as selectable marker”, Plant Cell Rep. 1995, 14(7):450-4; Christiansen P, et al. “A rapid and efficient transformation protocol for the grass  Brachypodium distachyon ”, Plant Cell Rep. 2005 March; 23(10-11):751-8. Epub 2004 Oct. 19; and Păcurar D I, et al. “A high-throughput Agrobacterium-mediated transformation system for the grass model species  Brachypodium distachyon  L”, Transgenic Res. 2008 17(5):965-75; each of which is fully incorporated herein by reference in its entirety. 
     The pQ6sVN vector, in addition to the multiple cloning sites (MCS) between the promoter and terminator, contains additional restriction sites (PmeI, I-SceI, and BsaI) downstream of the expression cassette to allow cloning of a second expression cassette into the vector. 
     Stacking two genes (two expression cassettes) into pQ6sVN was performed as follows: the first gene was cloned into pQ6sVN via the MCS. The second gene was cloned into the vector pUCsVN. Genes cloned into this high copy vector via its MCS are flanked by a 35S promoter and a NOS terminator—the same elements found in the pQ6sVN vector. The expression cassette of pUCsVN is flanked by restriction sites for PmeI, I-SceI and BsaI. The cassette containing the second gene was excised from pUCsVN by digestion with one of the aforementioned enzymes, and ligated into the pQ6sVN plasmid already carrying the first gene, linearized with the same restriction enzyme. This resulted in a single vector harboring two “stacked” cassettes. The sequences and orientations of both cassettes were verified by Sanger sequencing and restriction digests. 
     In case genomic DNA was cloned, the genes were amplified by direct PCR on genomic DNA extracted from leaf tissue using the DNAeasy kit (Qiagen Cat. No. 69104). 
     Table 10 hereinbelow provides a list of the gene cloned, including gene name, the organism from which the gene is derived, the SEQ ID NO. of the primer used and the SEQ ID NO. of the gene polynucleotide and encoded polypeptide. 
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 Cloned genes 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 Polyn. 
                 Polyp. 
               
               
                 Gene 
                   
                 Primers used 
                 SEQ ID 
                 SEQ ID 
               
               
                 Name 
                 Organism 
                 SEQ ID NOs 
                 NO 
                 NO 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 LFS82 
                 
                   Zea mays 
                 
                 535, 561, 574, 588 
                 63 
                 349 
               
               
                 LFS83 
                 
                   Triticum aestivum 
                 
                 536, 562, 575, 589 
                 64 
                 350 
               
               
                 LFS84 
                 
                   Triticum aestivum 
                 
                 — 
                 65 
                 310 
               
               
                 LFS86 
                 
                   Zea mays 
                 
                 — 
                 66 
                 311 
               
               
                 LFS89 
                 
                   Triticum aestivum 
                 
                 — 
                 67 
                 313 
               
               
                 LFS90 
                 
                   Aegilops tauschii 
                 
                 511, 537, 563, 576 
                 68 
                 351 
               
               
                 LFS91 
                 
                   Aegilops tauschii 
                 
                 512, 538 
                 69 
                 315 
               
               
                 LFS92 
                 
                   Aegilops tauschii 
                 
                 513, 539 
                 70 
                 352 
               
               
                 LFS93 
                 
                   Aegilops tauschii 
                 
                 — 
                 71 
                 317 
               
               
                 LFS94 
                 
                   Aegilops tauschii 
                 
                 514, 540, 564 
                 72 
                 353 
               
               
                 LFS95 
                 
                   Aegilops tauschii 
                 
                 515, 541, 565, 577 
                 73 
                 354 
               
               
                 LFS96 
                 
                   Aegilops tauschii 
                 
                 516, 542 
                 74 
                 355 
               
               
                 LFS97 
                 
                   Aegilops tauschii 
                 
                 517, 543, 566, 578 
                 75 
                 356 
               
               
                 LFS98 
                 
                   Aegilops tauschii 
                 
                 518, 544, 567, 579 
                 76 
                 357 
               
               
                 LFS99 
                 
                   Aegilops tauschii 
                 
                 519, 545, 568, 580 
                 77 
                 358 
               
               
                 LFS100 
                 
                   Aegilops tauschii 
                 
                 520, 546, 569, 581 
                 78 
                 359 
               
               
                 LFS102 
                 
                   Aegilops tauschii 
                 
                 521, 547, 582 
                 79 
                 325 
               
               
                 LFS104 
                 
                   Aegilops tauschii 
                 
                 547, 582, 522, 548 
                 80 
                 360 
               
               
                 LFS105 
                 
                   Aegilops tauschii 
                 
                 590, 591 
                 307 
                 510 
               
               
                 LFS106 
                 
                   Aegilops tauschii 
                 
                 523, 549 
                 81 
                 327 
               
               
                 LFS107 
                 
                   Aegilops tauschii 
                 
                 524, 550 
                 82 
                 361 
               
               
                 LFS109 
                 
                   Aegilops tauschii 
                 
                 525, 551 
                 83 
                 362 
               
               
                 LFS110 
                 
                   Aegilops tauschii 
                 
                 — 
                 84 
                 330 
               
               
                 LFS111 
                 
                   Aegilops tauschii 
                 
                 — 
                 85 
                 331 
               
               
                 LFS112 
                 
                   Aegilops tauschii 
                 
                 — 
                 304 
                 507 
               
               
                 LFS113 
                 
                   Aegilops tauschii 
                 
                 526, 552, 570, 583 
                 86 
                 363 
               
               
                 LFS114 
                 
                   Aegilops tauschii 
                 
                 527, 553, 571, 584 
                 87 
                 364 
               
               
                 LFS115 
                 
                   Aegilops tauschii 
                 
                 — 
                 88 
                 334 
               
               
                 LFS116 
                 
                   Aegilops tauschii 
                 
                 528, 554, 572, 585 
                 89 
                 335 
               
               
                 LFS117 
                 
                   Aegilops tauschii 
                 
                 529, 555 
                 90 
                 365 
               
               
                 LFS121 
                 
                   Setaria italica 
                 
                 — 
                 91 
                 337 
               
               
                 LFS122 
                 
                   Setaria italica 
                 
                 530, 556, 573, 586 
                 92 
                 338 
               
               
                 LFS123 
                 
                   Setaria italica 
                 
                 531, 557, 587 
                 93 
                 366 
               
               
                 LFS124 
                 
                   Setaria italica 
                 
                 — 
                 94 
                 340 
               
               
                 LFS125 
                 
                   Setaria italica 
                 
                 532, 558 
                 95 
                 367 
               
               
                 LFS126 
                 
                   Setaria italica 
                 
                 — 
                 96 
                 342 
               
               
                 LFS127 
                 
                   Setaria italica 
                 
                 — 
                 97 
                 343 
               
               
                 LFS128 
                 
                   Setaria italica 
                 
                 533, 559 
                 98 
                 368 
               
               
                 LFS129 
                 
                   Setaria italica 
                 
                 534, 560 
                 99 
                 345 
               
               
                   
               
               
                 “polyn.” = polynucleotide; 
               
               
                 “polyp.” = polypeptide. 
               
            
           
         
       
     
     Example 6: Transformation of  Brachypodium distachyon  Plants with the Polynucleotides of the Invention 
     Similar to the  Arabidopsis  model plant,  Brachypodium distachyon  has several features that recommend it as a model plant for functional genomics studies, especially in the grasses. Traits that make it an ideal model include its small genome (˜160 Mbp for a diploid genome and 355 Mbp for a polyploidy genome), small physical stature, a short lifecycle, and few growth requirements.  Brachypodium  is related to the major cereal grain species but is understood to be more closely related to the Triticeae (wheat, barley) than to the other cereals.  Brachypodium , with its polyploidy accessions, can serve as an ideal model for these grains (whose genomic size and complexity is a major barrier to biotechnological improvement). 
       Brachypodium distachyon  embryogenic calli were transformed using the procedure described by Vogel and Hill (2008. High-efficiency  Agrobacterium -mediated transformation of  Brachypodium distachyon  inbred line Bd21-3. Plant Cell Rep 27:471-478); Vain et al (2008 . Agrobacterium -mediated transformation of the temperate grass  Brachypodium distachyon  (genotype Bd21) for T-DNA insertional mutagenesis. Plant Biotechnology J 6: 236-245), and Vogel J, et al. (2006.  Agrobacterium  mediated transformation and inbred line development in the model grass  Brachypodium distachyon . Plant Cell Tiss Org. Cult. 85:199-211), each of which is fully incorporated herein by reference, with some minor modifications, which are briefly summarized hereinbelow. 
     Callus initiation—Immature spikes (about 2 months after seeding) were harvested at the very beginning of seeds filling. Spikes were then husked and surface sterilized with 3% NaClO containing 0.1% Tween 20, shaken on a gyratory shaker at low speed for 20 minutes. Following three rinses with sterile distilled water, embryos were excised under a dissecting microscope in a laminar flow hood using fine forceps. 
     Excised embryos (size ˜0.3 mm, bell shaped) were placed on callus induction medium (CIM) [LS salts (Linsmaier, E. M. &amp; Skoog, F. 1965. Physiol. Plantarum 18, 100) and vitamins plus 3% sucrose, 6 mg/L CuSO 4 , 2.5 mg/l 2,4-Dichlorophenoxyacetic Acid, pH 5.8 and 0.25% phytagel (Sigma)] with the scutellum side down, 50 or 100 embryos on a plate, and incubated at 28° C. in the dark. One week later, the embryonic calli were cleaned from emerging shoots and somatic calli, and subcultured onto fresh CIM medium. During culture, yellowish embryogenic calli (EC) appear and were further selected (e.g., picked and transferred) for further incubation in the same conditions for additional 2 weeks. Twenty-five pieces of sub-cultured calli were then separately placed on 90×15 mm petri plates, and incubated as before for three additional weeks. 
     Transformation—As described in Vogel and Hill (2008, Supra), Agrobacterium is scraped off 2-day-old MGL plates (plates with the MGL medium which contains: Tryptone 5 gr/L, Yeast Extract 2.5 gr/L, NaCl 5 gr/L, D-Mannitol 5 g/l, MgSO 4 *7H 2 O 0.204 gr/L, K 2 HPO 4  0.25 gr/L, Glutamic Acid 1.2 gr/L, Plant Agar 7.5 gr/L) and resuspended in liquid MS medium supplemented with 200 μM acetosyringone to an optic density (OD) at 600 nm (OD 600 ) of 0.6 to 1.0. Once the desired OD was attained, 1 ml of 10% Synperonic PE/F68 (Sigma) per 100 ml of inoculation medium is added. 
     To begin inoculation, 300 callus pieces were placed in approximately 12 plates (25 callus pieces in each plate) and covered with the Agrobacterium suspension (8-10 ml). The callus was incubated in the Agrobacterium suspension for 5 to 20 minutes. After incubation, the Agrobacterium suspension was aspirated off and the calli were then transferred into co-cultivation plates, prepared by placing a sterile 7-cm diameter filter paper in an empty 90×15 mm petri plate. The calli pieces are then gently distributed on the filter paper. One co-cultivation plate is used for two starting callus plates (50 initial calli pieces). The co-cultivation plates were then sealed with Parafilm M® or a plastic wrap [e.g., Saran™ wrap (Dow Chemical Company)] and incubated at 24° C. in the dark for 3 days. 
     The callus pieces were then individually transferred into CIM medium as described above, which was further supplemented with 200 mg/L Ticarcillin (to kill the Agrobacterium) and Bialaphos (5 mg/L) or Hygromycin B (40 mg/L) (for selection of the transformed resistant embryogenic calli sections), and incubated at 28° C. in the dark for 14 days. 
     The calli pieces were then transferred to shoot induction media (SIM; LS salts and vitamins plus 3% Maltose monohydrate) supplemented with 400 mg/L Ticarcillin, Bialaphos (5 mg/L) or Hygromycin B (40 mg/L), Indol-3-acetic acid (IAA) (0.25 mg/L), and 6-Benzylaminopurine (BAP) (1 mg/L), and were cultivated in conditions as described below. After 10-15 days calli were sub-cultured on the same fresh media for additional 10-15 days (total of 20-30 days). At each sub-culture all the pieces from a single callus were kept together to maintain their independence and were incubated under the following conditions: light to a level of 60 lE m −2  s −1 , a 16-hours light, 8-hours dark photoperiod and a constant 24° C. temperature. During the period of 20 to 30 days from the beginning of cultivation of calli on shoot induction media (SIM) plantlets start to emerge from the transformed calli. 
     When plantlets were large enough to handle without damage, they were transferred to plates containing the above-mentioned shoot induction media (SIM) with Bialaphos or Hygromycin B. Each plantlet is considered as a different event. After two weeks of growth, the plantlets were transferred to 2-cm height Petri plates (De Groot, Catalog No. 60-664160) containing MSnoH media (MS salts 4.4 gr/L, sucrose 30 gr/L, supplemented with Hygromycin B (40 mg/L) and Ticarcillin (400 mg/L). Roots usually appear within 2 weeks. Rooted and non-rooted plants were transferred to a fresh MSnoH media supplemented with Hygromycin B and Ticarcillin as described above. In case roots do not appear in the non-rooted plants after two weeks on the MSnoH media (which is supplemented with Hygromycin B and Ticarcillin), then the non-rooted plants are further transferred to the rooting induction medium [RIM; MS salts and vitamins 4.4 gr/L, sucrose 30 gr/L with Ticarcillin 400 mg/L, Indol-3-acetic acid (IAA) (1 mg/L), and a-Naphthalene acetic acid (NAA) (2 mg/L)]. After additional two weeks of incubation at 24° C., the plantlets are transferred to 0.5 modified RIM medium [MS modified salts 4.4 gr/L, MS vitamins 103 mg/L, sucrose 30 gr/L with α-Tocopherol (2 mg/L), Indol-3-acetic acid (IAA) (1 mg/L), and a-Naphthalene acetic acid (NAA) (2 mg/L)] and are incubated at 28° C. for additional 15-20 days, till the roots appear. 
     If needed, in the tillering stage the plantlets can grow axillary tillers and eventually become bushy on the above-mentioned media (SIM) without Bialaphos or Hygromycin B. Each bush from the same plant (event ID) is then divided to tissue culture boxes (“Humus”) containing “rooting medium” [MS basal salts, 3% sucrose, 3 gr/L phytagel, 2 mg/L α-Naphthalene Acetic Acid (NAA) and 1 mg/L IAA and Ticarcillin 400 mg/L, PH 5.8]. All plants in a “Humus box” are individual plants of the same transformation event. 
     When plantlets establish roots, they are transplanted to the soil and grown in the greenhouse. Before transfer to greenhouse, 20 randomly selected events are tested every month for expression of the BAR_GA gene (SEQ ID NO:39, BAR gene) which is responsible for resistance to Bialaphos, using AgraStrip® LL strip test seed check (Romer labs). Briefly, the expression of the BAR gene is determined as follows: Leaves (about 0.5 cm long leave) are grounded using a pellet pestle in an Eppendorf tube containing 150 μl of water until the water turns green in color. A strip test is then added to the Eppendorf tube and the results are read within 30-60 seconds. Appearance of two pink bands means that the plant is transgenic. On the other hand, appearance of one pink band means that the plant is not transgenic or not expressing BAR gene. 
     To verify the transgenic status of plants containing the gene of interest, Ti plants are subjected to PCR as previously described by Vogel et al. 2006 [ Agrobacterium  mediated transformation and inbred line development in the model grass  Brachypodium distachyon . Plant Cell Tiss Org. Cult. 85:199-211]. 
     Example 7: Validation Assays 
     The transgenic  Brachypodium  plants obtained as described hereinabove were used to validate the effect of the transformed gene(s) on fungal penetration and spreading within the plant by evaluation of fungal penetration and spreading within inoculated seedlings grown under controlled conditions. 
     Each validation assay evaluates the gene performance by quantitative and/or qualitative measure of specific traits as described in Table 11 below. 
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Allocation of fungal parameters to specific traits 
               
            
           
           
               
               
               
               
            
               
                   
                 # 
                 Parameters 
                 Traits 
               
               
                   
                   
               
               
                   
                 1 
                 Fungal biomass in root 
                 Fungal presence in tissue 
               
               
                   
                   
               
            
           
         
       
     
     The validation assay was performed with inoculated transgenic plants grown under controlled conditions till seedling stage (1-2 tillers). 
     Transgenic  Brachypodium  seeds were sown in trays contained sterilized vermiculite soaked with 0.1% BASTA solution for selection of transgenic plants. The trays were placed in the refrigerator at 4° C. for cold treatment for 3 days (stratification step) following by trays placement in controlled growth chamber for germination up to 5 days (26° C.±1, humidity of 50%). 
     The viable transgenic seedlings were inoculated with  Fusarium verticillioides  (Fv 149 strain, GFP-tagged and bar-resistant) spore solution (10 6 /ml) and then transplanted to 180 cm 3  pots filled with sterilized vermiculite. The plants were grown for 7 days in the growth chamber. 
     For fungal biomass recovery, roots externally sterilized and then grinded. Serial dilutions of the extracts from each one of the biological repeats (1 biological repeat is a pool of 6 roots) were plated in Petri dishes and incubated for 5 days at 25° C. Developing colonies were counted to provide Colony Forming Units (CFU) using a florescent binocular and data were transformed to logarithmic scale for further analysis. 
     Fungal Biomass was computed as Log CFU of fungal root extraction 7 days after inoculation 
     Statistical analyses—To identify genes conferring significantly improved tolerance to fungal penetration and fungal spreading, the results obtained from the transgenic plants were compared to those obtained from control plants. To identify outperforming genes and constructs, results from the independent transformation events tested were analyzed separately. Analysis was conducted on the log CFU data using Student&#39;s t-test. The JMP statistics software package was used (Version 5.2.1, SAS Institute Inc., Cary, N.C., USA). Table 12 hereinbelow shows the reduction in CFU in the tested infected plants and its significance compared to control plants. 
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 Validation results (single genes): reduction of fungi burden in 
               
               
                   Brachypodium  plant infected with  Fusarium verticilloides   
               
            
           
           
               
               
               
               
            
               
                 Gene 
                   
                 % change of logCFU relative to control 
                 % change of CFU 
               
               
                 Name 
                 Origin 
                 (p value) 
                 relative to control 
               
               
                   
               
               
                 LFS90 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 13869.1: 11.45% (significant, p_value = 0.00) 
                 Event 13869.1: 179.54% 
               
               
                   
                   
                 Event 13870.1: 6.93% (significant, p_value = 0.02) 
                 Event 13870.1: 86.38% 
               
               
                   
                   
                 Event 13865.1: 9.40% (significant, p_value = 0.00) 
                 Event 13865.1: 132.57% 
               
               
                   
                   
                 Event 13866.1: 18.38% (significant, p_value = 0.00) 
                 Event 13866.1: 420.96% 
               
               
                   
                   
                 Event 13868.1: 10.57% (significant, p_value = 0.00) 
                 Event 13868.1: 158.22% 
               
               
                   
                   
                 Event 13874.1: 9.78% (significant, p_value = 0.00) 
                 Event 13874.1: 140.59% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 13869.1: −1.72% (not significant, p_value = 0.25) 
                 Event 13869.1: -14.02% 
               
               
                   
                   
                 Event 13865.1: −2.28% (not significant, p_value = 0.18) 
                 Event 13865.1: −18.22% 
               
               
                   
                   
                 Event 13866.1: 3.23% (not significant, p_value = 0.11) 
                 Event 13866.1: 32.90% 
               
               
                   
                   
                 Event 13868.1: −3.61% (significant, p_value = 0.08) 
                 Event 13868.1: −27.22% 
               
               
                   
                   
                 Event 13874.1: 0.44% (not significant, p_value = 0.44) 
                 Event 13874.1: 3.95% 
               
               
                 LFS91 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 13884.1: 8.87% (significant, p_value = 0.00) 
                 Event 13884.1: 134.98% 
               
               
                   
                   
                 Event 13883.1: −6.99% (significant, p_value = 0.01) 
                 Event 13883.1: −49.02% 
               
               
                   
                   
                 Event 13885.1: −3.76% (not significant, p_value = 0.10) 
                 Event 13885.1: −30.38% 
               
               
                   
                   
                 Event 13891.1: 0.26% (not significant, p_value = 0.46) 
                 Event 13891.1: 2.55% 
               
               
                   
                   
                 Event 13890.1: 9.90% (significant, p_value = 0.00) 
                 Event 13890.1: 159.62% 
               
               
                   
                   
                 Event 13894.1: 1.62% (not significant, p_value = 0.29) 
                 Event 13894.1: 16.93% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 13883.1: −9.31% (significant, p_value = 0.00) 
                 Event 13883.1: −61.15% 
               
               
                 LFS92 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14188.1: −0.38% (not significant, p_value = 0.44) 
                 Event 14188.1: −3.18% 
               
               
                   
                   
                 Event 14202.1: −4.87% (significant, p_value = 0.08) 
                 Event 14202.1: −33.67% 
               
               
                   
                   
                 Event 14152.1: −5.65% (significant, p_value = 0.04) 
                 Event 14152.1: −37.92% 
               
               
                   
                   
                 Event 14155.1: −1.66% (not significant, p_value = 0.31) 
                 Event 14155.1: −13.09% 
               
               
                   
                   
                 Event 14187.1: −1.17% (not significant, p_value = 0.37) 
                 Event 14187.1: −9.42% 
               
               
                   
                   
                 Event 14201.1: −0.06% (not significant, p_value = 0.50) 
                 Event 14201.1: −0.52% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14202.1: −1.85% (not significant, p_value = 0.29) 
                 Event 14202.1: −16.59% 
               
               
                   
                   
                 Event 14152.1: −11.40% (significant, p_value = 0.00) 
                 Event 14152.1: −67.31% 
               
               
                 LFS93 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14149.1: −2.25% (not significant, p_value = 0.28) 
                 Event 14149.1: −18.91% 
               
               
                   
                   
                 Event 14150.1: 10.52% (significant, p_value = 0.00) 
                 Event 14150.1: 166.28% 
               
               
                   
                   
                 Event 14108.1: −1.06% (not significant, p_value = 0.38) 
                 Event 14108.1: −9.38% 
               
               
                   
                   
                 Event 14147.1: −5.21% (significant, p_value = 0.07) 
                 Event 14147.1: −38.44% 
               
               
                   
                   
                 Event 14156.1: 8.77% (significant, p_value = 0.01) 
                 Event 14156.1: 126.22% 
               
               
                   
                   
                 Event 14157.1: 7.58% (significant, p_value = 0.01) 
                 Event 14157.1: 102.48% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14147.1: −3.07% (not significant, p_value = 0.14) 
                 Event 14147.1: −25.88% 
               
               
                 LFS94 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14165.1: 4.96% (significant, p_value = 0.07) 
                 Event 14165.1: 49.56% 
               
               
                   
                   
                 Event 14164.1: 2.87% (not significant, p_value = 0.19) 
                 Event 14164.1: 26.25% 
               
               
                   
                   
                 Event 14162.1: 0.03% (not significant, p_value = 0.50) 
                 Event 14162.1: 0.23% 
               
               
                   
                   
                 Event 14171.1: 8.46% (significant, p_value = 0.01) 
                 Event 14171.1: 98.73% 
               
               
                   
                   
                 Event 14172.1: 8.30% (significant, p_value = 0.01) 
                 Event 14172.1: 96.09% 
               
               
                   
                   
                 Event 14168.1: 3.84% (not significant, p_value = 0.12) 
                 Event 14168.1: 36.52% 
               
               
                 LFS95 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14213.1: 9.19% (significant, p_value = 0.01) 
                 Event 14213.1: 135.70% 
               
               
                   
                   
                 Event 14212.1: 4.42% (not significant, p_value = 0.12) 
                 Event 14212.1: 51.10% 
               
               
                   
                   
                 Event 14208.1: 5.86% (significant, p_value = 0.05) 
                 Event 14208.1: 72.71% 
               
               
                   
                   
                 Event 14205.1: 0.68% (not significant, p_value = 0.43) 
                 Event 14205.1: 6.50% 
               
               
                   
                   
                 Event 14210.1: 6.97% (significant, p_value = 0.03) 
                 Event 14210.1: 91.54% 
               
               
                   
                   
                 Event 14214.1: −5.60% (significant, p_value = 0.06) 
                 Event 14214.1: −40.68% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14214.1: 6.12% (significant, p_value = 0.04) 
                 Event 14214.1: 82.23% 
               
               
                 LFS96 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14017.34: −10.34% (significant, p_value = 0.00) 
                 Event 14017.34: −63.12% 
               
               
                   
                   
                 Event 14018.29: −7.66% (significant, p_value = 0.01) 
                 Event 14018.29: −52.23% 
               
               
                   
                   
                 Event 14014.38: −18.53% (significant, p_value = 0.00) 
                 Event 14014.38: −83.26% 
               
               
                   
                   
                 Event 14015.40: −2.21% (not significant, p_value = 0.25) 
                 Event 14015.40: −19.17% 
               
               
                   
                   
                 Event 14019.34: −8.21% (significant, p_value = 0.01) 
                 Event 14019.34: −54.70% 
               
               
                   
                   
                 Event 14020.36: −5.72% (significant, p_value = 0.04) 
                 Event 14020.36: −42.40% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14017.34: −1.34% (not significant, p_value = 0.28) 
                 Event 14017.34: −13.44% 
               
               
                   
                   
                 Event 14018.29: 2.48% (not significant, p_value = 0.15) 
                 Event 14018.29: 30.61% 
               
               
                   
                   
                 Event 14014.38: −2.69% (not significant, p_value = 0.13) 
                 Event 14014.38: −25.17% 
               
               
                   
                   
                 Event 14019.34: −2.40% (not significant, p_value = 0.15) 
                 Event 14019.34: −22.82% 
               
               
                   
                   
                 Event 14020.36: −0.85% (not significant, p_value = 0.36) 
                 Event 14020.36: −8.74% 
               
               
                 LFS97 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 13932.1: −1.43% (not significant, p_value = 0.28) 
                 Event 13932.1: −12.81% 
               
               
                   
                   
                 Event 13938.1: −2.60% (not significant, p_value = 0.15) 
                 Event 13938.1: −22.09% 
               
               
                   
                   
                 Event 13936.1: −1.96% (not significant, p_value = 0.22) 
                 Event 13936.1: −17.12% 
               
               
                   
                   
                 Event 13937.1: −5.62% (significant, p_value = 0.01) 
                 Event 13937.1: −41.63% 
               
               
                   
                   
                 Event 13939.1: 1.19% (not significant, p_value = 0.32) 
                 Event 13939.1: 12.11% 
               
               
                   
                   
                 Event 13933.1: −0.78% (not significant, p_value = 0.38) 
                 Event 13933.1: −7.17% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 13937.1: 3.78% (not significant, p_value = 0.13) 
                 Event 13937.1: 41.87% 
               
               
                   
                   
                 Event 13937.1: −6.16% (significant, p_value = 0.00) 
                 Event 13937.1: −48.55% 
               
               
                 LFS98 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 13911.1: −0.83% (not significant, p_value = 0.42) 
                 Event 13911.1: −7.43% 
               
               
                   
                   
                 Event 13917.1: −2.20% (not significant, p_value = 0.22) 
                 Event 13917.1: −18.51% 
               
               
                   
                   
                 Event 13910.1: −5.18% (significant, p_value = 0.03) 
                 Event 13910.1: −38.17% 
               
               
                   
                   
                 Event 13909.1: −0.66% (not significant, p_value = 0.43) 
                 Event 13909.1: −5.97% 
               
               
                   
                   
                 Event 13915.1: 8.77% (significant, p_value = 0.00) 
                 Event 13915.1: 125.80% 
               
               
                   
                   
                 Event 13919.1: 6.10% (significant, p_value = 0.01) 
                 Event 13919.1: 76.17% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 13910.1: −4.21% (not significant, p_value = 0.12) 
                 Event 13910.1: −34.81% 
               
               
                 LFS99 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14138.1: 12.65% (significant, p_value = 0.00) 
                 Event 14138.1: 158.53% 
               
               
                   
                   
                 Event 14137.1: 6.25% (significant, p_value = 0.02) 
                 Event 14137.1: 59.92% 
               
               
                   
                   
                 Event 14140.1: 12.90% (significant, p_value = 0.00) 
                 Event 14140.1: 163.35% 
               
               
                   
                   
                 Event 14134.1: 8.96% (significant, p_value = 0.00) 
                 Event 14134.1: 95.88% 
               
               
                   
                   
                 Event 14135.1: −4.95% (significant, p_value = 0.05) 
                 Event 14135.1: −31.05% 
               
               
                   
                   
                 Event 14142.1: 24.44% (significant, p_value = 0.00) 
                 Event 14142.1: 526.43% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14138.1: −2.38% (not significant, p_value = 0.23) 
                 Event 14138.1: −20.09% 
               
               
                   
                   
                 Event 14137.1: 13.83% (significant, p_value = 0.00) 
                 Event 14137.1: 268.06% 
               
               
                   
                   
                 Event 14140.1: 9.65% (significant, p_value = 0.00) 
                 Event 14140.1: 148.18% 
               
               
                   
                   
                 Event 14135.1: 0.57% (not significant, p_value = 0.46) 
                 Event 14135.1: 5.54% 
               
               
                   
                   
                 Event 14141.1: −0.95% (not significant, p_value = 0.35) 
                 Event 14141.1: −8.60% 
               
               
                   
                   
                 Event 14142.1: 5.81% (significant, p_value = 0.06) 
                 Event 14142.1: 72.84% 
               
               
                 LFS100 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 13984.27: 8.05% (significant, p_value = 0.01) 
                 Event 13984.27: 125.39% 
               
               
                   
                   
                 Event 14070.35: −2.27% (not significant, p_value = 0.27) 
                 Event 14070.35: −20.44% 
               
               
                   
                   
                 Event 14052.30: −3.67% (not significant, p_value = 0.14) 
                 Event 14052.30: −30.97% 
               
               
                   
                   
                 Event 14051.31: −2.02% (not significant, p_value = 0.30) 
                 Event 14051.31: −18.41% 
               
               
                   
                   
                 Event 14077.38: −4.53% (significant, p_value = 0.10) 
                 Event 14077.38: −36.71% 
               
               
                   
                   
                 Event 13987.36: −4.48% (not significant, p_value = 0.10) 
                 Event 13987.36: −36.40% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14077.38: −1.98% (not significant, p_value = 0.27) 
                 Event 14077.38: −18.25% 
               
               
                 LFS104 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 13943.39: −0.15% (not significant, p_value = 0.47) 
                 Event 13943.39: −1.54% 
               
               
                   
                   
                 Event 14068.24: −2.02% (not significant, p_value = 0.29) 
                 Event 14068.24: −18.43% 
               
               
                   
                   
                 Event 14069.22: 2.54% (not significant, p_value = 0.27) 
                 Event 14069.22: 29.23% 
               
               
                   
                   
                 Event 14022.32: −11.15% (significant, p_value = 0.00) 
                 Event 14022.32: −67.60% 
               
               
                   
                   
                 Event 14023.24: −9.31% (significant, p_value = 0.02) 
                 Event 14023.24: −60.99% 
               
               
                   
                   
                 Event 14067.17: 4.44% (not significant, p_value = 0.14) 
                 Event 14067.17: 56.62% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14022.32: −19.32% (significant, p_value = 0.00) 
                 Event 14022.32: −86.85% 
               
               
                   
                   
                 Event 14023.24: −20.99% (significant, p_value = 0.00) 
                 Event 14023.24: −88.96% 
               
               
                 LFS105 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14026.1: −8.60% (significant, p_value = 0.00) 
                 Event 14026.1: −56.52% 
               
               
                   
                   
                 Event 14035.1: −1.32% (not significant, p_value = 0.29) 
                 Event 14035.1: −12.04% 
               
               
                   
                   
                 Event 14031.1: −3.52% (significant, p_value = 0.09) 
                 Event 14031.1: −28.86% 
               
               
                   
                   
                 Event 14025.1: −2.91% (not significant, p_value = 0.12) 
                 Event 14025.1: −24.57% 
               
               
                   
                   
                 Event 14034.1: −2.54% (not significant, p_value = 0.16) 
                 Event 14034.1: −21.80% 
               
               
                   
                   
                 Event 14029.1: −2.34% (not significant, p_value = 0.17) 
                 Event 14029.1: −20.30% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14026.1: −4.00% (significant, p_value = 0.08) 
                 Event 14026.1: −32.37% 
               
               
                   
                   
                 Event 14031.1: 2.04% (not significant, p_value = 0.23) 
                 Event 14031.1: 22.08% 
               
               
                 LFS106 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 13993.33: 6.18% (significant, p_value = 0.02) 
                 Event 13993.33: 80.50% 
               
               
                   
                   
                 Event 13996.23: 8.88% (significant, p_value = 0.00) 
                 Event 13996.23: 133.43% 
               
               
                   
                   
                 Event 14000.41: 7.34% (significant, p_value = 0.01) 
                 Event 14000.41: 101.56% 
               
               
                   
                   
                 Event 14003.37: 2.66% (not significant, p_value = 0.21) 
                 Event 14003.37: 28.96% 
               
               
                   
                   
                 Event 13999.42: 2.15% (not significant, p_value = 0.24) 
                 Event 13999.42: 22.80% 
               
               
                   
                   
                 Event 14001.37: 11.19% (significant, p_value = 0.00) 
                 Event 14001.37: 191.28% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 13993.33: −7.16% (significant, p_value = 0.05) 
                 Event 13993.33: −52.83% 
               
               
                   
                   
                 Event 13996.23: −5.75% (significant, p_value = 0.09) 
                 Event 13996.23: −45.32% 
               
               
                   
                   
                 Event 14000.41: −2.56% (not significant, p_value = 0.27) 
                 Event 14000.41: −23.57% 
               
               
                   
                   
                 Event 14001.37: −4.58% (not significant, p_value = 0.14) 
                 Event 14001.37: −38.15% 
               
               
                 LFS107 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 13951.32: 10.34% (significant, p_value = 0.00) 
                 Event 13951.32: 153.93% 
               
               
                   
                   
                 Event 13948.31: 11.64% (significant, p_value = 0.00) 
                 Event 13948.31: 185.33% 
               
               
                   
                   
                 Event 13946.32: 8.99% (significant, p_value = 0.00) 
                 Event 13946.32: 124.83% 
               
               
                   
                   
                 Event 13956.45: 11.85% (significant, p_value = 0.00) 
                 Event 13956.45: 190.80% 
               
               
                   
                   
                 Event 13960.33: 4.67% (significant, p_value = 0.06) 
                 Event 13960.33: 52.37% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 13951.32: 1.24% (not significant, p_value = 0.34) 
                 Event 13951.32: 11.52% 
               
               
                   
                   
                 Event 13948.31: 3.36% (significant, p_value = 0.09) 
                 Event 13948.31: 34.45% 
               
               
                   
                   
                 Event 13946.32: 1.90% (not significant, p_value = 0.21) 
                 Event 13946.32: 18.24% 
               
               
                   
                   
                 Event 13956.45: 4.76% (significant, p_value = 0.02) 
                 Event 13956.45: 52.17% 
               
               
                 LFS109 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14193.1: −5.39% (significant, p_value = 0.00) 
                 Event 14193.1: −39.83% 
               
               
                   
                   
                 Event 14194.1: 1.98% (not significant, p_value = 0.14) 
                 Event 14194.1: 20.52% 
               
               
                   
                   
                 Event 14197.1: 0.05% (not significant, p_value = 0.48) 
                 Event 14197.1: 0.51% 
               
               
                   
                   
                 Event 14192.1: 0.49% (not significant, p_value = 0.38) 
                 Event 14192.1: 4.67% 
               
               
                   
                   
                 Event 14198.1: −5.18% (significant, p_value = 0.00) 
                 Event 14198.1: −38.60% 
               
               
                   
                   
                 Event 14191.1: −1.98% (not significant, p_value = 0.15) 
                 Event 14191.1: −16.99% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14193.1: 2.69% (not significant, p_value = 0.16) 
                 Event 14193.1: 30.08% 
               
               
                 LFS110 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14254.1: −3.90% (significant, p_value = 0.07) 
                 Event 14254.1: −33.07% 
               
               
                   
                   
                 Event 14283.1: 0.61% (not significant, p_value = 0.40) 
                 Event 14283.1: 6.45% 
               
               
                   
                   
                 Event 14255.1: 4.83% (significant, p_value = 0.03) 
                 Event 14255.1: 64.37% 
               
               
                   
                   
                 Event 14284.1: −6.43% (significant, p_value = 0.01) 
                 Event 14284.1: −48.43% 
               
               
                   
                   
                 Event 14250.1: 0.60% (not significant, p_value = 0.41) 
                 Event 14250.1: 6.40% 
               
               
                   
                   
                 Event 14252.1: 0.17% (not significant, p_value = 0.47) 
                 Event 14252.1: 1.81% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14254.1: 0.22% (not significant, p_value = 0.44) 
                 Event 14254.1: 2.24% 
               
               
                   
                   
                 Event 14284.1: −0.97% (not significant, p_value = 0.32) 
                 Event 14284.1: −9.28% 
               
               
                 LFS111 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14064.1: 17.84% (significant, p_value = 0.00) 
                 Event 14064.1: 433.55% 
               
               
                   
                   
                 Event 14058.1: −9.20% (significant, p_value = 0.00) 
                 Event 14058.1: −57.84% 
               
               
                   
                   
                 Event 14060.1: −8.68% (significant, p_value = 0.00) 
                 Event 14060.1: −55.73% 
               
               
                   
                   
                 Event 14073.1: 10.65% (significant, p_value = 0.00) 
                 Event 14073.1: 171.77% 
               
               
                   
                   
                 Event 14057.1: −5.24% (significant, p_value = 0.03) 
                 Event 14057.1: −38.82% 
               
               
                   
                   
                 Event 14059.1: 13.43% (significant, p_value = 0.00) 
                 Event 14059.1: 252.78% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14058.1: −2.16% (not significant, p_value = 0.20) 
                 Event 14058.1: −17.33% 
               
               
                   
                   
                 Event 14057.1: 3.96% (not significant, p_value = 0.17) 
                 Event 14057.1: 42.13% 
               
               
                   
                   
                 Event 14060.1: −0.35% (not significant, p_value = 0.45) 
                 Event 14060.1: −3.33% 
               
               
                 LFS112 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14307.1: −5.30% (significant, p_value = 0.01) 
                 Event 14307.1: −40.36% 
               
               
                   
                   
                 Event 14309.1: −1.49% (not significant, p_value = 0.25) 
                 Event 14309.1: −13.54% 
               
               
                   
                   
                 Event 14298.1: 0.65% (not significant, p_value = 0.40) 
                 Event 14298.1: 6.53% 
               
               
                   
                   
                 Event 14301.1: −4.38% (significant, p_value = 0.03) 
                 Event 14301.1: −34.78% 
               
               
                   
                   
                 Event 14302.1: −9.07% (significant, p_value = 0.00) 
                 Event 14302.1: −58.75% 
               
               
                   
                   
                 Event 14305.1: −1.56% (not significant, p_value = 0.24) 
                 Event 14305.1: −14.12% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14307.1: 0.90% (not significant, p_value = 0.33) 
                 Event 14307.1: 9.44% 
               
               
                   
                   
                 Event 14301.1: −3.62% (significant, p_value = 0.04) 
                 Event 14301.1: −30.53% 
               
               
                   
                   
                 Event 14302.1: −4.12% (significant, p_value = 0.02) 
                 Event 14302.1: −33.92% 
               
               
                 LFS113 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 13974.29: 4.15% (significant, p_value = 0.05) 
                 Event 13974.29: 44.75% 
               
               
                   
                   
                 Event 13979.31: 4.39% (significant, p_value = 0.04) 
                 Event 13979.31: 47.89% 
               
               
                 LFS114 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14179.1: −14.48% (significant, p_value = 0.00) 
                 Event 14179.1: −75.12% 
               
               
                   
                   
                 Event 14181.1: −4.51% (significant, p_value = 0.03) 
                 Event 14181.1: −35.14% 
               
               
                   
                   
                 Event 14175.1: −8.02% (significant, p_value = 0.00) 
                 Event 14175.1: −53.70% 
               
               
                   
                   
                 Event 14184.1: −7.70% (significant, p_value = 0.00) 
                 Event 14184.1: −52.26% 
               
               
                   
                   
                 Event 14174.1: −20.43% (significant, p_value = 0.00) 
                 Event 14174.1: −85.95% 
               
               
                   
                   
                 Event 14177.1: −9.03% (significant, p_value = 0.00) 
                 Event 14177.1: −58.00% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14179.1: −1.72% (not significant, p_value = 0.28) 
                 Event 14179.1: −15.43% 
               
               
                   
                   
                 Event 14181.1: −1.90% (not significant, p_value = 0.26) 
                 Event 14181.1: −16.90% 
               
               
                   
                   
                 Event 14175.1: 0.26% (not significant, p_value = 0.46) 
                 Event 14175.1: 2.59% 
               
               
                   
                   
                 Event 14184.1: −1.44% (not significant, p_value = 0.31) 
                 Event 14184.1: −13.09% 
               
               
                   
                   
                 Event 14174.1: −3.09% (not significant, p_value = 0.14) 
                 Event 14174.1: −25.97% 
               
               
                   
                   
                 Event 14177.1: −2.13% (not significant, p_value = 0.23) 
                 Event 14177.1: −18.75% 
               
               
                 LFS115 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14260.1: 1.19% (not significant, p_value = 0.34) 
                 Event 14260.1: 12.64% 
               
               
                   
                   
                 Event 14261.1: −0.84% (not significant, p_value = 0.39) 
                 Event 14261.1: −8.09% 
               
               
                   
                   
                 Event 14268.1: 4.73% (significant, p_value = 0.05) 
                 Event 14268.1: 60.69% 
               
               
                   
                   
                 Event 14259.1: 0.10% (not significant, p_value = 0.49) 
                 Event 14259.1: 0.97% 
               
               
                   
                   
                 Event 14263.1: −8.76% (significant, p_value = 0.00) 
                 Event 14263.1: −58.48% 
               
               
                   
                   
                 Event 14262.1: 4.26% (significant, p_value = 0.08) 
                 Event 14262.1: 53.37% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14263.1: −4.66% (significant, p_value = 0.01) 
                 Event 14263.1: −37.40% 
               
               
                 LFS116 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 13962.41: −2.38% (not significant, p_value = 0.30) 
                 Event 13962.41: −20.21% 
               
               
                   
                   
                 Event 13898.1: −2.67% (not significant, p_value = 0.27) 
                 Event 13898.1: −22.39% 
               
               
                   
                   
                 Event 13904.1: −7.55% (significant, p_value = 0.04) 
                 Event 13904.1: −51.20% 
               
               
                   
                   
                 Event 13961.30: −4.26% (not significant, p_value = 0.17) 
                 Event 13961.30: −33.27% 
               
               
                   
                   
                 Event 13902.1: −3.32% (not significant, p_value = 0.23) 
                 Event 13902.1: −27.05% 
               
               
                   
                   
                 Event 13903.1: −7.12% (significant, p_value = 0.06) 
                 Event 13903.1: −49.13% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 13904.1: −1.77% (not significant, p_value = 0.29) 
                 Event 13904.1: −16.48% 
               
               
                   
                   
                 Event 13903.1: −0.56% (not significant, p_value = 0.44) 
                 Event 13903.1: −5.52% 
               
               
                 LFS117 
                 
                   Aegilops 
                 
                 first assay: 
                 first assay: 
               
               
                   
                   
                 Event 14246.1: −5.66% (significant, p_value = 0.05) 
                 Event 14246.1: −42.61% 
               
               
                   
                   
                 Event 14203.1: 3.03% (not significant, p_value = 0.19) 
                 Event 14203.1: 34.55% 
               
               
                   
                   
                 Event 14245.1: −9.05% (significant, p_value = 0.00) 
                 Event 14245.1: −58.81% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14246.1: 3.99% (not significant, p_value = 0.14) 
                 Event 14246.1: 44.59% 
               
               
                   
                   
                 Event 14245.1: −1.48% (not significant, p_value = 0.34) 
                 Event 14245.1: −12.76% 
               
               
                 LFS122 
                 Foxtail 
                 first assay: 
                 first assay: 
               
               
                   
                 millet 
                 Event 13926.1: 10.35% (significant, p_value = 0.00) 
                 Event 13926.1: 160.27% 
               
               
                   
                   
                 Event 13880.1: 14.50% (significant, p_value = 0.00) 
                 Event 13880.1: 281.82% 
               
               
                   
                   
                 Event 13879.1: 12.68% (significant, p_value = 0.00) 
                 Event 13879.1: 222.64% 
               
               
                   
                   
                 Event 13908.1: 6.91% (significant, p_value = 0.01) 
                 Event 13908.1: 89.30% 
               
               
                   
                   
                 Event 13906.1: 0.21% (not significant, p_value = 0.47) 
                 Event 13906.1: 1.99% 
               
               
                   
                   
                 Event 13907.1: −3.63% (significant, p_value = 0.09) 
                 Event 13907.1: −28.52% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 13907.1: −9.24% (significant, p_value = 0.00) 
                 Event 13907.1: −60.88% 
               
               
                 LFS123 
                 Foxtail 
                 first assay: 
                 first assay: 
               
               
                   
                 millet 
                 Event 14127.1: 3.23% (not significant, p_value = 0.11) 
                 Event 14127.1: 37.61% 
               
               
                   
                   
                 Event 14130.1: −0.64% (not significant, p_value = 0.40) 
                 Event 14130.1: −6.18% 
               
               
                   
                   
                 Event 14125.1: −13.66% (significant, p_value = 0.00) 
                 Event 14125.1: −74.11% 
               
               
                   
                   
                 Event 14121.1: −5.44% (significant, p_value = 0.02) 
                 Event 14121.1: −41.63% 
               
               
                   
                   
                 Event 14122.1: 7.25% (significant, p_value = 0.00) 
                 Event 14122.1: 104.81% 
               
               
                   
                   
                 Event 14131.1: 1.34% (not significant, p_value = 0.30) 
                 Event 14131.1: 14.13% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14125.1: 0.49% (not significant, p_value = 0.47) 
                 Event 14125.1: 4.42% 
               
               
                   
                   
                 Event 14121.1: −0.47% (not significant, p_value = 0.39) 
                 Event 14121.1: −4.06% 
               
               
                 LFS125 
                 Foxtail 
                 first assay: 
                 first assay: 
               
               
                   
                 millet 
                 Event 14043.1: 0.01% (not significant, p_value = 0.49) 
                 Event 14043.1: 0.05% 
               
               
                   
                   
                 Event 14056.1: −6.59% (significant, p_value = 0.02) 
                 Event 14056.1: −46.26% 
               
               
                   
                   
                 Event 14044.1: −6.57% (significant, p_value = 0.02) 
                 Event 14044.1: −46.15% 
               
               
                   
                   
                 Event 14048.1: 1.23% (not significant, p_value = 0.34) 
                 Event 14048.1: 12.26% 
               
               
                   
                   
                 Event 14042.1: 1.20% (not significant, p_value = 0.34) 
                 Event 14042.1: 11.95% 
               
               
                   
                   
                 Event 14053.1: −4.57% (significant, p_value = 0.08) 
                 Event 14053.1: −34.98% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14056.1: −6.84% (significant, p_value = 0.02) 
                 Event 14056.1: −50.22% 
               
               
                   
                   
                 Event 14044.1: −6.73% (significant, p_value = 0.02) 
                 Event 14044.1: −49.65% 
               
               
                   
                   
                 Event 14053.1: −3.49% (not significant, p_value = 0.15) 
                 Event 14053.1: −29.92% 
               
               
                 LFS126 
                 Foxtail 
                 first assay: 
                 first assay: 
               
               
                   
                 millet 
                 Event 14081.1: −4.75% (significant, p_value = 0.02) 
                 Event 14081.1: −37.78% 
               
               
                   
                   
                 Event 14089.1: −9.88% (significant, p_value = 0.00) 
                 Event 14089.1: −62.77% 
               
               
                   
                   
                 Event 14085.1: 6.71% (significant, p_value = 0.00) 
                 Event 14085.1: 95.51% 
               
               
                   
                   
                 Event 14082.1: −0.40% (not significant, p_value = 0.43) 
                 Event 14082.1: −3.88% 
               
               
                   
                   
                 Event 14086.1: −10.60% (significant, p_value = 0.00) 
                 Event 14086.1: −65.34% 
               
               
                   
                   
                 Event 14087.1: 3.21% (significant, p_value = 0.08) 
                 Event 14087.1: 37.82% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14081.1: −5.25% (significant, p_value = 0.06) 
                 Event 14081.1: −41.44% 
               
               
                   
                   
                 Event 14089.1: 2.31% (not significant, p_value = 0.23) 
                 Event 14089.1: 26.61% 
               
               
                   
                   
                 Event 14086.1: −4.17% (significant, p_value = 0.10) 
                 Event 14086.1: −34.67% 
               
               
                 LFS127 
                 Foxtail 
                 first assay: 
                 first assay: 
               
               
                   
                 millet 
                 Event 14102.1: −1.42% (not significant, p_value = 0.32) 
                 Event 14102.1: −14.65% 
               
               
                   
                   
                 Event 14104.1: −6.45% (significant, p_value = 0.01) 
                 Event 14104.1: −51.20% 
               
               
                   
                   
                 Event 14101.1: −3.76% (significant, p_value = 0.08) 
                 Event 14101.1: −34.14% 
               
               
                   
                   
                 Event 14096.1: −4.07% (significant, p_value = 0.07) 
                 Event 14096.1: −36.39% 
               
               
                   
                   
                 Event 14098.1: −7.82% (significant, p_value = 0.00) 
                 Event 14098.1: −58.07% 
               
               
                   
                   
                 Event 14100.1: −9.83% (significant, p_value = 0.00) 
                 Event 14100.1: −66.48% 
               
               
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                 Event 14104.1: 2.31% (not significant, p_value = 0.29) 
                 Event 14104.1: 24.66% 
               
               
                   
                   
                 Event 14101.1: −0.33% (not significant, p_value = 0.47) 
                 Event 14101.1: −3.06% 
               
               
                   
                   
                 Event 14096.1: 7.82% (significant, p_value = 0.03) 
                 Event 14096.1: 110.92% 
               
               
                   
                   
                 Event 14098.1: −1.41% (not significant, p_value = 0.37) 
                 Event 14098.1: −12.56% 
               
               
                   
                   
                 Event 14100.1: 2.72% (not significant, p_value = 0.26) 
                 Event 14100.1: 29.66% 
               
               
                 LFS128 
                 Foxtail 
                 first assay: 
                 first assay: 
               
               
                   
                 millet 
                 Event 14222.1: −0.79% (not significant, p_value = 0.40) 
                 Event 14222.1: −7.05% 
               
               
                   
                   
                 Event 14223.1: −2.67% (not significant, p_value = 0.19) 
                 Event 14223.1: −21.93% 
               
               
                   
                   
                 Event 14225.1: −1.43% (not significant, p_value = 0.32) 
                 Event 14225.1: −12.39% 
               
               
                   
                   
                 Event 14226.1: −2.56% (not significant, p_value = 0.17) 
                 Event 14226.1: −21.10% 
               
               
                   
                   
                 Event 14229.1: −0.93% (not significant, p_value = 0.38) 
                 Event 14229.1: −8.23% 
               
               
                   
                   
                 Event 14220.1: 2.35% (not significant, p_value = 0.22) 
                 Event 14220.1: 24.38% 
               
               
                   
               
               
                 “CFU” = Colony Forming Unit 
               
            
           
         
       
     
     An increase in the resistance of plants to fungal diseases may be achieved by two genes stack expression, which are addressing the same or different mode of action. 
     Two different combinations of gene stacking were expressed in  Brachypodium  plants. The combination of LFS57 (disclosed in WO 2018/131037), a gene linked to programmed cell death, and LFSS86 disclosed in the present invention, a gene related to plant defense signaling activation, addresses the effect of stacking two different mode of actions directed to increase resistance. 
     The combination of LFS46 (disclosed in WO 2018/131037) and LFS84 disclosed in the present invention addresses the effect of stacking two genes coding for enzymes processing the same metabolite involved in defense response signaling therefore increasing the efficacy of the reaction. 
     Table 13 hereinbelow summarizes the reduction of fungi burden in the transgenic  Brachypodium  plants comprising the stacked genes infected with  Fusarium verticilloides . 
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 Validation results (stacked genes): reduction of fungi burden in 
               
               
                   Brachypodium  plant infected with  Fusarium verticilloides   
               
            
           
           
               
               
               
               
               
            
               
                 Construct 
                 Origin 
                 Origin 
                 % change of logCFU relative to control 
                 % change of CFU 
               
               
                 Name 
                 gene 1 
                 gene 2 
                 (p-value) 
                 relative to control 
               
               
                   
               
               
                 Stack: LFS57 &amp; LFS86 
                 Sorghum 
                 Maize 
                 first assay: 
                 first assay: 
               
               
                   
                   
                   
                 Event 13342.1: −11.07% (significant, 
                 Event 13342.1: −66.14% 
               
               
                   
                   
                   
                 p_value = 0.00) 
                 Event 13345.1: −41.40% 
               
               
                   
                   
                   
                 Event 13345.1: −5.46% (significant, 
                 Event 13339.1: −74.12% 
               
               
                   
                   
                   
                 p_value = 0.04) 
                 Event 13344.1: 93.66% 
               
               
                   
                   
                   
                 Event 13339.1: −13.82% (significant, 
                 Event 13331.1: 3.85% 
               
               
                   
                   
                   
                 p_value = 0.00) 
                 Event 13340.1: −56.20% 
               
               
                   
                   
                   
                 Event 13344.1: 6.76% (significant, 
               
               
                   
                   
                   
                 p_value = 0.01) 
               
               
                   
                   
                   
                 Event 13331.1: 0.39% (not significant, 
               
               
                   
                   
                   
                 p_value = 0.45) 
               
               
                   
                   
                   
                 Event 13340.1: −8.44% (significant, 
               
               
                   
                   
                   
                 p value = 0.00) 
               
               
                   
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                   
                 Event 13342.1: −2.88% (not significant, 
                 Event 13342.1: −23.99% 
               
               
                   
                   
                   
                 p_value = 0.13) 
                 Event 13345.1: −22.59% 
               
               
                   
                   
                   
                 Event 13345.1: −2.69% (not significant, 
                 Event 13339.1: −30.57% 
               
               
                   
                   
                   
                 p_value = 0.15) 
               
               
                   
                   
                   
                 Event 13339.1: −3.83% (significant, 
               
               
                   
                   
                   
                 p_value = 0.07) 
               
               
                 Stack: LFS46 &amp; LFS84 
                 Sorghum 
                 Wheat 
                 first assay: 
                 first assay: 
               
               
                   
                   
                   
                 Event 13859.1: −7.47% (significant, 
                 Event 13859.1: −52.31% 
               
               
                   
                   
                   
                 p_value = 0.01) 
                 Event 13852.1: −0.79% 
               
               
                   
                   
                   
                 Event 13852.1: −0.08% (not significant, 
                 Event 13848.1: −26 99% 
               
               
                   
                   
                   
                 p_value = 0.49) 
               
               
                   
                   
                   
                 Event 13848.1: −3.18% (not significant, 
               
               
                   
                   
                   
                 p_value = 0.17) 
                 Event 13850.1: −72.23% 
               
               
                   
                   
                   
                 Event 13850.1: −12.93% (significant, 
                 Event 13851.1: 53.34% 
               
               
                   
                   
                   
                 p_value = 0.00) 
               
               
                   
                   
                   
                 Event 13851.1: 4.31% (not significant, 
               
               
                   
                   
                   
                 p_value = 0.11) 
               
               
                   
                   
                   
                 second assay: 
                 second assay: 
               
               
                   
                   
                   
                 Event 13859.1: −4.10% (not significant, 
                 Event 13859.1: −31.75% 
               
               
                   
                   
                   
                 p_value = 0.13) 
                 Event 13850.1: −74.82% 
               
               
                   
                   
                   
                 Event 13850.1: −14.82% (significant, 
               
               
                   
                   
                   
                 p_value = 0.00) 
               
               
                   
               
               
                 “CFU” = Colony Forming Unit 
               
            
           
         
       
     
     Example 8: Overexpression of a Polypeptide by Genome Editing 
     Over-expression of a polypeptide according to certain embodiments of the present invention can be achieved using methods of gene editing. One example of such approach includes editing a selected genomic region as to express the polypeptide of interest. In the current example, the target genomic region is the maize locus GRMZM2G069095 (based on genome version  Zea mays  AGPv3) and the polypeptide to be over-expressed is the maize LFS127_H4 comprising the amino acid sequence set forth in SEQ ID NO:495 encoded by the polynucleotide having the nucleic acid sequence set forth in SEQ ID NO:289. It is to be explicitly understood that other genome loci can be used as targets for genome editing for over-expressing other polypeptides of the invention based on the same principles. 
       FIG. 2A  depicts the sequence of the endogenous 5′ upstream flanking region of the genomic sequence GRMZM2G069095 (SEQ ID NO:12) and  FIG. 2B  depicts the sequence of the endogenous 3′-downstream flanking region of this genomic locus (SEQ ID NO:13).  FIG. 2C  depicts the sequence of the 5′-UTR gRNA (SEQ ID NO:10) and  FIG. 2D  depicts the sequence of the 5′-UTR gRNA without NGG nucleotides following the 3 nucleotides after the Cas9 cutting (SEQ ID NO:14).  FIG. 2E  depicts the sequence of the 3′-UTR gRNA (SEQ ID NO:11) and  FIG. 2F  depicts the sequence of the 3′-UTR gRNA after cut (SEQ ID NO:15).  FIG. 2G  depicts the coding sequence (from the “ATG” start codon to the “TAG” termination codon, marked by bold and underlined) of the desired LFS127_H4 sequence (SEQ ID NO:289) encoding the polypeptide set forth by SEQ ID NO:495. 
     The complete exemplary repair template (SEQ ID NO:16) is depicted in  FIG. 2H . The repair template includes: (1) the upstream flanking region (1 kbp) sequence including part of the gRNA after cutting (SEQ ID NO:14; shown in bold and italics); (2) 5′ UTR of genomic DNA from Cas9 cutting site to ATG; (3) the coding sequence (CDS) of the desired LFS127_H4 sequence (SEQ ID NO:17) marked in lower case with the start (ATG) and the stop (TGA) codons marked in bold and underlined; (4) 3′ UTR of genomic DNA from the stop codon to Cas9 cutting site including the predicted part of the gRNA after cutting (SEQ ID NO:15), shown in bold and underlined and (5) the downstream flanking region (1 kbp) sequence. 
     The repair template is delivered into the cell type of interest along with the 5′ and 3′guide RNA sequences (SEQ ID NO:10 and SEQ ID NO:11, respectively). 
     Example 9: Knock Out of a Polypeptide by Genome Editing 
     Knock-out of a polypeptide according to certain embodiments of the present invention can be achieved using methods of gene editing. 
     In the current example, the target genomic region is the maize ( Zea mays ) LFS132 comprising the amino acid sequence set forth in SEQ ID NO:348 encoded by the polynucleotide having the nucleic acid sequence set forth in SEQ ID NO:62. It is to be explicitly understood that other genome loci can be used as targets for genome editing for over-expressing other polypeptides of the invention based on the same principles. 
     The Crisper/CAS9 or similar systems can generate double stranded breaks (DSBs) at any genomic locus under the guidance of an engineered single-guide RNA when delivered into the cell type of interest. Non-homologous end-joining (NHEJ) in the absence of DNA template accompanied with the modification of target genomic repair the DSBs but tends to be prone to insertion and/or deletion (indel) mutations at the junctional site therefore causing frameshifts mutations that disrupt the targeted gene. ( FIG. 3E ) 
       FIG. 3A  depicts the sequence of the KO gRNA (SEQ ID NO:51);  FIG. 3C  depicts the coding sequence (from the “ATG” start codon to the “TGA” termination codon, marked by bold and underlined) of the desired LFS132 sequence (SEQ ID NO:53);  FIG. 3B  depicts the sequence of the KO gRNA after cut (SEQ ID NO:52);  FIG. 3D  (targeted region in bold) and  FIG. 3E  depict the anticipated change in the coding sequence of the exemplified KO gene (SEQ ID NO:54). 
     Section II: Bacterial Genes Associated with Fungal Resistance 
     Example 10: Isolating Soil Bacteria with Potential Antifungal Activity 
     Bacteria were isolated from soil in five different locations in Israel with agriculture history for  Fusaria  spp. related diseases, by a two-layer functional assay against  Fusarium verticillioides  (Fv). In brief, fungal spores were plated on PDA agar medium with a thin R2A medium (Reasoner D J &amp; Geldreich A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 1985 49:1-7) overlay containing different dilutions of soil washes. Active bacteria were isolated from lytic zones and further characterized for the antifungal activity in dual-culture confrontation assays against Fv on R2A agar plates. Table 14 presents a list of the bacteria isolated. In parenthesis is the percentage of similarity between the isolate and the known genus or species. 
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Bacterial isolates having antifungal activity 
               
            
           
           
               
               
            
               
                 Isolate 
                 Taxa Isolate 
               
               
                 No. 
                 (Percentage of similarity) 
               
               
                   
               
            
           
           
               
               
            
               
                 1 
                   Arthrobacter globiformis  (31.4) 
               
               
                 2 
                   Bacillus  (95.0) 
               
               
                 3 
                   Pseudomonas  (66.2) 
               
               
                 4 
                   Erwinia  (17.8) 
               
               
                 5 
                   Arthrobacter  (33.1) 
               
               
                 6 
                   Bacillus  (75.5) 
               
               
                 7 
                   Bacillus  (94.8) 
               
               
                 8 
                   Bacillus  (94.8) 
               
               
                 9 
                   Pseudomonas  (43.6) |  Massilia  (45.6) 
               
               
                 10 
                   Pseudomonas  (43.6) | Massilia  (45.6) 
               
               
                 11 
                   Pseudomonas  (44.0) 
               
               
                 12 
                   Pseudomonas  (61.4) 
               
               
                 13 
                   Pseudomonas  (61.4) 
               
               
                 14 
                   Pseudomonas  (61.4) 
               
               
                 15 
                   Pseudomonas  (52.7) | Bacillus subtilis  (80.1) 
               
               
                 16 
                   Bacillus  (78.4) 
               
               
                 17 
                   Bacillus  (95.2) 
               
               
                 18 
                   Bacillus  (95.1) 
               
               
                 19 
                   Bacillus  (93.5) 
               
               
                 20 
                   Pseudomonas  (50.6) 
               
               
                 21 
                   Erwinia  (17.9) 
               
               
                 22 
                   Lysobacter   —   capsici  (85.2)  Erwinia  (18.0) 
               
               
                 23 
                   Arthrobacter  (36.1) 
               
               
                 24 
                   Arthrobacter   —   globiformis  (29.2) 
               
               
                 25 
                   Pseudomonas  (63.8)  Lysobacter  (22.5) 
               
               
                 26 
                   Pseudomonas  (63.8)  Lysobacter  (22.5) 
               
               
                 27 
                   Pseudomonas  (59.2) 
               
               
                 28 
                   Pseudomonas  (59.2) 
               
               
                 29 
                   Pseudomonas  (59.2) 
               
               
                 30 
                   Chryseobacterium   —   daeyuense  (28.1) 
               
               
                 31 
                   Chryseobacterium   —   daeyuense  (28.1) 
               
               
                 32 
                   Pseudomonas  (44.0) 
               
               
                 33 
                   Pseudomonas  (44.0) 
               
               
                 34 
                   Pseudomonas  (61.4) 
               
               
                 35 
                   Pseudomonas  (60.9) 
               
               
                 36 
                   Pseudomonas  (60.9) 
               
               
                 37 
                   Pseudomonas  (58.9)  Bacillus  (77.2) 
               
               
                 38 
                   Bacillus  (74.0) 
               
               
                 39 
                   Arthrobacter  (19.8) 
               
               
                 40 
                   Pantoea   —   agglomerans  (35.7) 
               
               
                 41 
                   Curtobaderium _UNCCL17(82.3) |  Pantoea   —   agglomerans   
               
               
                   
                 (91.6) 
               
               
                 42 
                   Pantoea  (83.6) 
               
               
                 43 
                   Pantoea  (83.6) 
               
               
                 44 
                   Pseudomonas alcaligenes  (38.6) 
               
               
                 45 
                   Pseudomonas  (50.2) 
               
               
                 46 
                   Pseudomonas  (41.2) 
               
               
                 47 
                   Pseudomonas  (85.4) 
               
               
                 48 
                   Leifsonia  (11.9) |  Clavibacter michiganensis  (9.3) 
               
               
                 49 
                   Microbacterium  (19.8) 
               
               
                 50 
                   Microbacterium  (19.8) 
               
               
                 51 
                   Pseudomonas  (60.1) 
               
               
                 52 
                   Bacillus  (94.5) 
               
               
                 53 
                   Pseudomonas  s (50.1) 
               
               
                 54 
                   Pseudomonas  (40.5) 
               
               
                 55 
                   Pseudomonas  (40.5) 
               
               
                 56 
                   Pseudomonas  (59.1) 
               
               
                 57 
                   Pseudomonas  s (59.1) 
               
               
                 58 
                   Bacillus  (92.4) 
               
               
                 59 
                   Pseudomonas  (44.0) 
               
               
                 60 
                   Bacillus  (92.4) 
               
               
                 61 
                   Pseudomonas  (44.0) 
               
               
                 62 
                   Pseudomonas  (56.0) 
               
               
                 63 
                   Bacillus  (95.0) 
               
               
                 64 
                   Bacillus  (74.0) 
               
               
                 65 
                   Pseudomonas  (41.1) 
               
               
                 66 
                   Pseudomonas  (41.1) 
               
               
                 67 
                   Pseudomonas  (41.1) 
               
               
                 68 
                   Pseudomonas  (60.1) 
               
               
                 69 
                   Pseudomonas  (60.1) 
               
               
                 70 
                   Pseudomonas  (60.1) 
               
               
                 71 
                   Pantoea  (100); 
               
               
                 72 
                   Bacillus  (100); 
               
               
                 73 
                   Arthrobacter  (100); unclassified; 
               
               
                 74 
                   Paenibacillus  (100); unclassified; 
               
               
                 75 
                   Paenibacillus  (100); unclassified; 
               
               
                 76 
                   Pseudomonas  (100);  Pseudomonas frederiksbergensis  (98) 
               
               
                 77 
                   Erwinia  (91); unclassified; 
               
               
                 78 
                   Bacillus  (100) 
               
               
                 79 
                 
                   Pseudomonas chloraphis 
                 
               
               
                 80 
                 Alcaligenaceae (100); unclassified 
               
               
                 81 
                 
                   Bacillus 
                   — 
                   axarquiensis 
                 
               
               
                 82 
                   Pseudomonas  spp. 
               
               
                 83 
                 
                   Pantoea agglomerans 
                 
               
               
                 84 
                 
                   Chryseobacterium 
                   — 
                   ginsenosidimutans 
                 
               
               
                 85 
                 
                   Bacillus 
                   — 
                   altitudinis 
                 
               
               
                   
               
            
           
         
       
     
     Example 11: Identifying Fungicidal Genes Eligible for Disease Control 
     The present inventors have identified 199 polynucleotides of bacterial origin that encode for proteins with potential antifungal activity. The antifungal activity of successfully purified soluble proteins was tested in an in vitro spore assay against  Fusarium verticilloides . The antifungal activity is further examined by expressing the bacterial genes in planta, and evaluating fungal resistance conferred by the exogenous polynucleotides. 
     The polynucleotides and polypeptides of some embodiments of the invention having the antifungal activity originate from bacterial isolate proprietary genomes as listed in Table 14 and public bacterial genomes that were incorporated into a unified database, also containing gene expression data produced ad hoc, as well as gene phylogeny, protein annotation, enzymatic function and pathway. 
     Genomic and Transcriptomic Profiling of Bacterial Isolates for Gene Discovery 
     Genomic Profiling 
     Genome assembly—genomes of all isolates were assembled using a proprietary pipeline. 
     Gene prediction—gene prediction was performed using Prokaryotic Dynamic Programming Genefinding Algorithm [Prodigal—BMC Bioinformatics. 2010 Mar. 8; 11(1):119]. 
     Gene annotation—Predicted genes and proteins were annotated using BLAST™ search [blast.ncbi.nlm.nih.gov/Blast.cgi] against NCBI nr (non-redundant protein sequence database) and by further analysis by InterPro [ebi.ac.uk/interpro/]. 
     Transcriptome Profiling 
     Eleven (11) bacterial strains (Serial Number 71, 72, 73, 74, 75, 77, 78, 79, 80, 83 of Table 14) were striped on a R2A medium in Petri dishes on the two opposite sides of a  Fusarium verticillioides  strip culture. Bacterial samples were collected after 3 days of co-culture after a clear inhibition zone was defined and the collected bacteria were used for transcriptomics analysis. Total RNA was extracted and submitted to Omega Bioservices contract lab for RNA sequencing. Transcriptomics data were evaluated for identification of highly expressed potential antifungal genes. 
     Differential Expression Data Analysis 
     RNA-seq uses next-generation sequencing (NGS) methods to sequence cDNA that has been derived from an RNA sample, and hence produces millions of short reads. These reads were then mapped onto a reference genome and the number of reads mapping within a genomic feature of interest (such as a gene) was used as a measure of the abundance of the feature in the analyzed sample, resulting in gene expression profile. According to the gene expression profile, a differential analysis was performed to identify genes with higher expression during fungal co-culture compared to pure bacteria cultures that are further associated with antifungal phenotypes. 
     Identification of Antifungal Genes from Bacterial Isolates 
     The 199 genes listed in Table 15 below were identified as potential antifungal and may confer fungal-resistance traits in planta. The Table shows the identified genes, their curated polynucleotide and polypeptide sequences. 
     
       
         
           
               
             
               
                 TABLE 15 
               
             
            
               
                   
               
               
                 Identified antifungal genes from bacterial isolates 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 Polyn. 
                 Polyp. 
               
               
                 Gene 
                   
                   
                 SEQ ID 
                 SEQ ID 
               
               
                 Name 
                 Gene Description 
                 Isolate Name 
                 NO 
                 NO 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 BLFS1 
                 antifungal protein 
                   Streptomyces clavuligerus  ATCC 
                 592 
                 2444 
               
               
                   
                   
                 27064 
               
               
                 BLFS2 
                 Hypothetical protein 
                 15627EM_META_Alkanindiges1| 
                 593 
                 2445 
               
               
                 BLFS3 
                 Hypothetical protein 
                 15627EM_META —   
                 594 
                 2446 
               
               
                   
                   
                   Enterococcus 10|16v1|scaffold580442 
               
               
                   
                   
                 G95 
               
               
                 BLFS4 
                 Hypothetical protein 
                 15627EM_META_ Klebsiella 4 
                 595 
                 2447 
               
               
                 BLFS5 
                 Hypothetical protein 
                   Actinosporangium _NRRL_B_3428 
                 596 
                 2448 
               
               
                 BLFS6 
                 Hypothetical protein 
                 
                   Actinosynnema mirum 
                 
                 597 
                 2449 
               
               
                 BLFS7 
                 peptidase C11 
                   Ammonifex   —   degensii _KC4 
                 598 
                 2450 
               
               
                   
                 clostripain 
               
               
                 BLFS8 
                 Hypothetical protein 
                 
                   Amycolatopsis azurea 
                 
                 599 
                 2451 
               
               
                 BLFS9 
                 oxidoreductase 
                 
                   Amycolatopsis azurea 
                 
                 600 
                 2452 
               
               
                 BLFS10 
                 oxidoreductase 
                   Amycolatopsis azurea  DSM 43854 
                 601 
                 2453 
               
               
                 BLFS11 
                 Hypothetical protein 
                   Arthrobacter  spp. 
                 602 
                 2454 
               
               
                 BLFS12 
                 Hypothetical protein 
                   Arthrobacter  spp. 
                 603 
                 2455 
               
               
                 BLFS13 
                 Hypothetical protein 
                   Arthrobacter  spp. 
                 604 
                 2456 
               
               
                 BLFS14 
                 alpha integrin 
                   Arthrobacter  spp. 
                 605 
                 2457 
               
               
                 BLFS15 
                 alpha integrin 
                   Arthrobacter _161MFSha2_1 
                 606 
                 2458 
               
               
                 BLFS16 
                 lysozyme 
                   Arthrobacter _161MFSha2_1 
                 607 
                 2459 
               
               
                 BLFS17 
                 D-alanyl-D-alanine 
                   Arthrobacter _161MFSha2_1 
                 608 
                 2460 
               
               
                   
                 carboxypeptidase 
               
               
                 BLFS18 
                 Hypothetical protein 
                   Bacillus  spp. 
                 609 
                 2461 
               
               
                 BLFS19 
                 peptidase p60 
                   Bacillus  spp. 
                 610 
                 2462 
               
               
                 BLFS20 
                 Cell division 
                   Bacillus  spp. 
                 611 
                 2463 
               
               
                   
                 suppressor protein 
               
               
                 BLFS21 
                 spoivb peptidase 
                   Bacillus  spp. 
                 612 
                 2464 
               
               
                 BLFS22 
                 Hypothetical protein 
                   Bacillus  spp. 
                 613 
                 2465 
               
               
                 BLFS23 
                 glycosyl hydrolase 
                   Bacillus  spp. 
                 614 
                 2466 
               
               
                   
                 family 18 
               
               
                 BLFS24 
                 Hypothetical protein 
                   Bacillus  spp. 
                 615 
                 2467 
               
               
                 BLFS25 
                 chitooligosaccharide 
                   Bacillus  spp. 
                 616 
                 2468 
               
               
                   
                 deacetylase 
               
               
                 BLFS26 
                 Hypothetical protein 
                   Bacillus  spp. 
                 617 
                 2469 
               
               
                 BLFS27 
                 large protein of pyocin 
                   Bacillus  spp. 
                 618 
                 2470 
               
               
                   
                 ap41 
               
               
                 BLFS29 
                 Hypothetical protein 
                   Bacillus   —   marmarensis _DSM_21297 
                 619 
                 2471 
               
               
                 BLFS30 
                 epsilon-toxin type b 
                   Bacillus   —   thuringiensis _serovar_indiana 
                 620 
                 2472 
               
               
                 BLFS31 
                 Hypothetical protein 
                 
                   Bacillus velezensis 
                 
                 621 
                 2473 
               
               
                 BLFS32 
                 Protease HtpX [htpX]. 
                 
                   Bacillus velezensis 
                 
                 622 
                 2474 
               
               
                 BLFS33 
                 zinc metalloprotease 
                 
                   Bacillus velezensis 
                 
                 623 
                 2475 
               
               
                 BLFS34 
                 Cell division 
                 
                   Bacillus velezensis 
                 
                 624 
                 2476 
               
               
                   
                 suppressor protein 
               
               
                 BLFS35 
                 Hypothetical protein 
                 
                   Bradyrhizobium japonicum 
                 
                 625 
                 2477 
               
               
                 BLFS36 
                 Hypothetical protein 
                 
                   Bradyrhizobium yuanmingense 
                 
                 626 
                 2478 
               
               
                 BLFS37 
                 beta/gamma crystallin 
                 
                   Brevibacillus laterosporus 
                 
                 627 
                 2479 
               
               
                   
                 family protein 
               
               
                 BLFS38 
                 jacalin-like lectin 
                 
                   Brevibacillus laterosporus 
                 
                 628 
                 2480 
               
               
                   
                 domain protein 
               
               
                 BLFS39 
                 toxin ETX 
                 
                   Brevibacillus laterosporus 
                 
                 629 
                 2481 
               
               
                 BLFS41 
                 chitobiase 
                 
                   Burkholderia gladioli 
                 
                 630 
                 2482 
               
               
                 BLFS42 
                 chitin-binding protein 
                 
                   Burkholderia gladioli 
                 
                 631 
                 2483 
               
               
                 BLFS43 
                 carboxypeptidase 
                 
                   Burkholderia gladioli 
                 
                 632 
                 2484 
               
               
                   
                 regulatory-like domain 
               
               
                   
                 protein 
               
               
                 BLFS44 
                 ricin-type beta-trefoil 
                 
                   Burkholderia gladioli 
                 
                 633 
                 2485 
               
               
                   
                 lectin domain protein 
               
               
                 BLFS45 
                 peptidase 
                 
                   Burkholderia gladioli 
                 
                 634 
                 2486 
               
               
                 BLFS46 
                 lysm domain protein 
                 
                   Burkholderia gladioli 
                 
                 635 
                 2487 
               
               
                 BLFS47 
                 Hypothetical protein 
                   Cellulosimicrobium  spp. 
                 636 
                 2488 
               
               
                 BLFS48 
                 peptidase m48 
                 
                   Collimonas arenae 
                 
                 637 
                 2489 
               
               
                 BLFS49 
                 Hypothetical protein 
                 
                   Collimonas arenae 
                 
                 638 
                 2490 
               
               
                 BLFS50 
                 chitin-binding protein 
                 
                   Collimonas 
                   — 
                   arenae 
                 
                 639 
                 2491 
               
               
                 BLFS51 
                 endoglucanase 
                 
                   Collimonas 
                   — 
                   arenae 
                 
                 640 
                 2492 
               
               
                 BLFS52 
                 Ricin B lectin 
                 
                   Dactylosporangium aurantiacum 
                 
                 641 
                 2493 
               
               
                 BLFS53 
                 Etx/MtX1 toxin 
                 
                   Dickeya 
                   — 
                   dadantii 
                 
                 642 
                 2494 
               
               
                 BLFS55 
                 beta/gamma crystallin 
                   Duganella _HH101 
                 643 
                 2495 
               
               
                   
                 family protein 
               
               
                 BLFS56 
                 aminopeptidase 
                   Duganella _HH101 
                 644 
                 2496 
               
               
                 BLFS57 
                 glycoside hydrolase 
                   Duganella _HH101 
                 645 
                 2497 
               
               
                   
                 family 12 
               
               
                 BLFS58 
                 17 kda surface antigen 
                   Duganella _HH101 
                 646 
                 2498 
               
               
                 BLFS59 
                 Hypothetical protein 
                 
                   Duganella 
                   — 
                   violaceinigra 
                 
                 647 
                 2499 
               
               
                 BLFS60 
                 Hypothetical protein 
                   Erwinia  spp. 
                 648 
                 2500 
               
               
                 BLFS61 
                 type i secretion target 
                   Erwinia  spp. 
                 649 
                 2501 
               
               
                 BLFS62 
                 murein hydrolase 
                   Erwinia  spp. 
                 650 
                 2502 
               
               
                   
                 activator nlpd 
               
               
                 BLFS63 
                 n-acetylmuramoyl-1- 
                   Erwinia  spp. 
                 651 
                 2503 
               
               
                   
                 alanine amidase amib 
               
               
                 BLFS64 
                 NLP/P60 protein 
                   Geobacillus  Y4_1MC1 
                 652 
                 2504 
               
               
                 BLFS65 
                 extracellular repeat 
                   Janthinobacterium _HH010 
                 653 
                 2505 
               
               
                   
                 containing protein 
               
               
                   
                 HAF family 
               
               
                 BLFS67 
                 Beta/gamma crystallin 
                   Janthinobacterium _HH103 
                 654 
                 2506 
               
               
                 BLFS68 
                 putative exported 
                   Janthinobacterium  MP5059B 
                 655 
                 2507 
               
               
                   
                 protein 
               
               
                 BLFS69 
                 heme adhesion protein 
                 
                   Janthinobacterium 
                   — 
                   Marseille 
                 
                 656 
                 2508 
               
               
                 BLFS70 
                 Hypothetical protein 
                 
                   Janthinobacterium 
                   — 
                   agaricidamnosum 
                 
                 657 
                 2509 
               
               
                 BLFS72 
                 Carboxypeptidase 
                   Lysobacter  spp. 
                 658 
                 2510 
               
               
                 BLFS73 
                 Hypothetical protein 
                   Paenibacillus  spp. 
                 659 
                 2511 
               
               
                 BLFS74 
                 m1-852 
                   Paenibacillus  spp. 
                 660 
                 2512 
               
               
                 BLFS75 
                 Serine hydrolase 
                   Paenibacillus  spp. 
                 661 
                 2513 
               
               
                 BLFS76 
                 Hypothetical protein 
                   Paenibacillus  spp. 
                 662 
                 2514 
               
               
                 BLFS77 
                 glycoside hydrolase 
                   Paenibacillus  spp. 
                 663 
                 2515 
               
               
                   
                 family 5 
               
               
                 BLFS79 
                 Hypothetical protein 
                   Paenibacillus  spp. 
                 664 
                 2516 
               
               
                 BLFS80 
                 Hypothetical protein 
                   Paenibacillus  spp. 
                 665 
                 2517 
               
               
                 BLFS82 
                 sspe 
                   Paenibacillus  spp. 
                 666 
                 2518 
               
               
                 BLFS83 
                 exoglucanase a 
                   Paenibacillus  spp. 
                 667 
                 2519 
               
               
                 BLFS84 
                 alpha/beta hydrolase 
                   Paenibacillus  spp. 
                 668 
                 2520 
               
               
                 BLFS85 
                 alpha-amylase 
                   Paenibacillus  spp. 
                 669 
                 2521 
               
               
                 BLFS86 
                 hypothetical protein 
                   Paenibacillus  spp. 
                 670 
                 2522 
               
               
                 BLFS87 
                 endoglucanase b 
                   Paenibacillus  spp. 
                 671 
                 2523 
               
               
                 BLFS88 
                 Hypothetical protein 
                   Paenibacillus  spp. 
                 672 
                 2524 
               
               
                 BLFS89 
                 endoglucanase 
                   Paenibacillus  spp. 
                 673 
                 2525 
               
               
                 BLFS90 
                 wgr domain- 
                   Paenibacillus  spp. 
                 674 
                 2526 
               
               
                   
                 containing protein 
               
               
                 BLFS91 
                 Hypothetical protein 
                   Paenibacillus  spp. 
                 675 
                 2527 
               
               
                 BLFS92 
                 spoivb peptidase 
                   Paenibacillus  spp. 
                 676 
                 2528 
               
               
                 BLFS93 
                 arabinogalactan endo- 
                   Paenibacillus  spp. 
                 677 
                 2529 
               
               
                   
                 1,4-beta-galactosidase 
               
               
                 BLFS94 
                 rhamnogalacturonan 
                   Paenibacillus  spp. 
                 678 
                 2530 
               
               
                   
                 endolyase 
               
               
                 BLFS95 
                 Hypothetical protein 
                   Paenibacillus  spp. 
                 679 
                 2531 
               
               
                 BLFS96 
                 Hypothetical protein 
                 
                   Paenibacillus curdlanolyticus 
                 
                 680 
                 2532 
               
               
                 BLFS97 
                 Hypothetical protein 
                 
                   Paenibacillus mucilaginosus 
                 
                 681 
                 2533 
               
               
                 BLFS98 
                 Hypothetical protein 
                   Pantoea  spp. 
                 682 
                 2534 
               
               
                 BLFS99 
                 uncharacterized 
                   Pantoea  spp. 
                 683 
                 2535 
               
               
                   
                 protein yhcn 
               
               
                 BLFS100 
                 uncharacterized 
                   Pantoea  spp. 
                 684 
                 2536 
               
               
                   
                 protein yhcn 
               
               
                 BLFS101 
                 metalloprotease loiP 
                   Pantoea  spp. 
                 685 
                 2537 
               
               
                 BLFS102 
                 Hypothetical protein 
                   Pantoea  spp. 
                 686 
                 2538 
               
               
                 BLFS103 
                 toxin tccc3 
                   Pantoea  spp. 
                 687 
                 2539 
               
               
                 BLFS104 
                 Hypothetical protein 
                   Pantoea  spp. 
                 688 
                 2540 
               
               
                 BLFS105 
                 protein of unknown 
                   Pantoea  spp. 
                 689 
                 2541 
               
               
                   
                 function (DUF1311} 
               
               
                 BLFS106 
                 uncharacterized 
                   Pantoea  spp. 
                 690 
                 2542 
               
               
                   
                 protein yqjd 
               
               
                 BLFS107 
                 polyketide synthase 
                   Pantoea  spp. 
                 691 
                 2543 
               
               
                 BLFS108 
                 toxin relE 
                   Pantoea  spp. 
                 692 
                 2544 
               
               
                 BLFS109 
                 hemolysin 
                   Pantoea  spp. 
                 693 
                 2545 
               
               
                 BLFS110 
                 hypothetical protein 
                   Pedobacter  V48 
                 694 
                 2546 
               
               
                 BLFS111 
                 hypothetical protein 
                 
                   Pseudogulbenkiania 
                   — 
                   ferrooxidans 
                 
                 695 
                 2547 
               
               
                   
                   
                 EGD HP2 
               
               
                 BLFS112 
                 Hypothetical protein 
                   Pseudomonas  spp. 
                 696 
                 2548 
               
               
                 BLFS113 
                 transpeptidase 
                   Pseudomonas  spp 
                 697 
                 2549 
               
               
                 BLFS114 
                 ATP-dependent zinc 
                   Pseudomonas  spp 
                 698 
                 2550 
               
               
                   
                 protease 
               
               
                 BLFS115 
                 probable periplasmic 
                   Pseudomonas  spp 
                 699 
                 2551 
               
               
                   
                 serine endoprotease 
               
               
                   
                 DegP-like 
               
               
                 BLFS116 
                 lysophospholipase 
                   Pseudomonas  spp 
                 700 
                 2552 
               
               
                 BLFS118 
                 peptidoglycan-binding 
                   Pseudomonas  spp 
                 701 
                 2553 
               
               
                   
                 protein 
               
               
                 BLFS119 
                 Hypothetical protein 
                   Pseudomonas  spp 
                 702 
                 2554 
               
               
                 BLFS120 
                 supernuclease toxin 2 
                   Pseudomonas  spp. 
                 703 
                 2555 
               
               
                   
                 family protein 
               
               
                 BLFS121 
                 glutamate 
                   Pseudomonas  spp 
                 704 
                 2556 
               
               
                   
                 carboxypeptidase 
               
               
                 BLFS122 
                 hpr 
                   Pseudomonas  spp 
                 705 
                 2557 
               
               
                 BLFS123 
                 peptidase c1 
                   Pseudomonas  spp 
                 706 
                 2558 
               
               
                 BLFS124 
                 tigr02448 family 
                   Pseudomonas  spp 
                 707 
                 2559 
               
               
                   
                 protein 
               
               
                 BLFS125 
                 peptidase 
                   Pseudomonas  spp 
                 708 
                 2560 
               
               
                 BLFS126 
                 Hypothetical protein 
                   Pseudomonas  spp 
                 709 
                 2561 
               
               
                 BLFS127 
                 Hypothetical protein 
                   Pseudomonas  spp 
                 710 
                 2562 
               
               
                 BLFS128 
                 peptidoglycan 
                   Pseudomonas  spp 
                 711 
                 2563 
               
               
                   
                 hydrolase flgj 
               
               
                 BLFS129 
                 alginate lyase 
                   Pseudomonas  spp 
                 712 
                 2564 
               
               
                 BLFS130 
                 leucine-rich repeat- 
                   Pseudomonas  spp.. 
                 713 
                 2565 
               
               
                   
                 containing protein 
               
               
                 BLFS131 
                 uncharacterized 
                 
                   Pseudomonas chlororaphis 
                 
                 714 
                 2566 
               
               
                   
                 protein ycfj 
               
               
                 BLFS132 
                 Hypothetical protein 
                 
                   Pseudomonas chlororaphis 
                 
                 715 
                 2567 
               
               
                 BLFS133 
                 Hypothetical protein 
                 
                   Pseudomonas chlororaphis 
                 
                 716 
                 2568 
               
               
                 BLFS134 
                 Protein of unknown 
                 
                   Pseudomonas chlororaphis 
                 
                 717 
                 2569 
               
               
                   
                 function DUF2782 
               
               
                 BLFS135 
                 multidrug transporter 
                 
                   Pseudomonas chlororaphis 
                 
                 718 
                 2570 
               
               
                 BLFS136 
                 Hypothetical protein 
                   Pseudoxanthomonas  J31 
                 719 
                 2571 
               
               
                 BLFS137 
                 membrane protein 
                   Rhodococcus  KB6 
                 720 
                 2572 
               
               
                 BLFS138 
                 uncharacterized 
                   Salinibacillus  spp. 
                 721 
                 2573 
               
               
                   
                 peptidase 
               
               
                 BLFS139 
                 peptidoglycan 
                   Salinibacillus  spp. 
                 722 
                 2574 
               
               
                   
                 endopeptidase 
               
               
                 BLFS140 
                 uncharacterized 
                   Salinibacillus  spp. 
                 723 
                 2575 
               
               
                   
                 lipoprotein ybbd 
               
               
                 BLFS141 
                 mannan endo-1,4-beta- 
                   Salinibacillus  spp. 
                 724 
                 2576 
               
               
                   
                 mannosidase 
               
               
                 BLFS142 
                 uncharacterized 
                   Salinibacillus  spp. 
                 725 
                 2577 
               
               
                   
                 protein ykvt 
               
               
                 BLFS143 
                 putative signal peptide 
                   Salinibacillus  spp. 
                 726 
                 2578 
               
               
                   
                 peptidase sppa 
               
               
                 BLFS144 
                 thermolysin 
                   Salinibacillus  spp. 
                 727 
                 2579 
               
               
                 BLFS145 
                 d-alanyl-d-alanine 
                   Salinibacillus  spp. 
                 728 
                 2580 
               
               
                   
                 carboxypeptidase dacb 
               
               
                 BLFS146 
                 1-ala--d-glu 
                   Salinibacillus  spp. 
                 729 
                 2581 
               
               
                   
                 endopeptidase 
               
               
                 BLFS147 
                 beta-n- 
                   Salinibacillus  spp. 
                 730 
                 2582 
               
               
                   
                 acetylglucosaminidase 
               
               
                 BLFS148 
                 metalloprotease loip 
                   Serratia   —   fonticola _AU_P3 
                 731 
                 2583 
               
               
                 BLFS149 
                 chitobiase 
                   Serratia   —   fonticola _LMG_7882 
                 732 
                 2584 
               
               
                 BLFS150 
                 lytic enzyme 
                   Stenotrophomonas   —   maltophilia _ATCC_19867 
                 733 
                 2585 
               
               
                 BLFS151 
                 peptidase S33 family 
                   Stenotrophomonas   —   maltophilia _S028 
                 734 
                 2586 
               
               
                   
                 protein 
               
               
                 BLFS152 
                 lipase 
                   Streptomyces _NRRL_F_4335 
                 735 
                 2587 
               
               
                 BLFS153 
                 peptidase 
                 
                   Streptomyces albus 
                 
                 736 
                 2588 
               
               
                 BLFS154 
                 peptidase s1 
                   Streptomyces   —   clavuligerus _ATCC_27064 
                 737 
                 2589 
               
               
                 BLFS155 
                 mannose-binding 
                 
                   Streptomyces griseorubens 
                 
                 738 
                 2590 
               
               
                   
                 protein 
               
               
                 BLFS156 
                 hypothetical protein 
                   Streptomyces   —   mobaraensis _NBRC_13819 
                 739 
                 2591 
               
               
                 BLFS157 
                 hypothetical protein 
                   Thalassobacter   —   arenae _DSM_19593 
                 740 
                 2592 
               
               
                 BLFS158 
                 bicupin, oxalate 
                   Virgibacillus _Vm_5 
                 741 
                 2593 
               
               
                   
                 decarboxylase family 
               
               
                 BLFS159 
                 large Ala/Gln-rich 
                   Xanthomonas   —   hortorum _pv_carotae_M081 
                 742 
                 2594 
               
               
                   
                 protein 
               
               
                 PUB6 
                 Hypothetical protein 
                 
                   Bacillus 
                   — 
                   weihenstephanensis 
                 
                 743 
                 2595 
               
               
                 PUB23 
                 Hypothetical protein 
                 
                   Bacillus cereus 
                 
                 744 
                 2596 
               
               
                 PUB31 
                 Hypothetical protein 
                   Bacillus   —   thuringiensis _serovar_andalousiensis 
                 745 
                 2597 
               
               
                 PUB65 
                 putative extracellular 
                   Variovorax   —   paradoxus  B4 
                 746 
                 2598 
               
               
                   
                 carbohydrate- 
               
               
                   
                 recognition protein 
               
               
                 ZLFS1 
                 Arthropod defensin 
                   Actinomyces _oral_taxon_171 
                 747 
                 2599 
               
               
                 ZLFS2 
                 peptidase m14 
                   Arthrobacter  spp. 
                 748 
                 2600 
               
               
                 ZLFS3 
                 probable 
                 
                   Bacillus 
                   — 
                   amyloliquefaciens 
                   — 
                   plantarum 
                 
                 749 
                 2601 
               
               
                   
                 peptidoglycan 
               
               
                   
                 endopeptidase lyte 
               
               
                 ZLFS5 
                 Hypothetical protein 
                   Burkholderia _A1 
                 750 
                 2602 
               
               
                 ZLFS6 
                 type vi secretion 
                   Collimonas   —   fungivorans _Ter331 
                 751 
                 2603 
               
               
                   
                 protein 
               
               
                 ZLFS7 
                 glycosyl hydrolase 
                   Collimonas   —   fungivorans _Ter331 
                 752 
                 2604 
               
               
                 ZLFS8 
                 chitin-binding protein 
                   Collimonas   —   fungivorans _Ter331 
                 753 
                 2605 
               
               
                 ZLFS9 
                 polyketide synthase- 
                   Collimonas   —   fungivorans _Ter331 
                 754 
                 2606 
               
               
                   
                 like protein 
               
               
                 ZLFS10 
                 Hypothetical protein 
                   Collimonas   —   fungivorans _Ter331 
                 755 
                 2607 
               
               
                 ZLFS11 
                 patatin 
                   Collimonas   —   fungivorans _Ter331 
                 756 
                 2608 
               
               
                 ZLFS12 
                 pseudomonalisin 
                   Collimonas   —   fungivorans _Ter331 
                 757 
                 2609 
               
               
                 ZLFS13 
                 Hypothetical protein 
                   Collimonas   —   fungivorans _Ter331 
                 758 
                 2610 
               
               
                 ZLFS14 
                 Hypothetical protein 
                   Collimonas   —   fungivorans _Ter331 
                 759 
                 2611 
               
               
                 ZLFS15 
                 Beta-barrel assembly- 
                   Collimonas   —   fungivorans _Ter331 
                 760 
                 2612 
               
               
                   
                 enhancing protease 
               
               
                   
                 [bepA|. 
               
               
                 ZLFS16 
                 d-alanyl-d-alanine 
                   Collimonas   —   fungivorans _Ter331 
                 761 
                 2613 
               
               
                   
                 carboxypeptidase dacd 
               
               
                 ZLFS17 
                 Antimicrobial protein 
                 
                   Herbidospora cretacea 
                 
                 762 
                 2614 
               
               
                   
                 MiAMP1 
               
               
                 ZLFS18 
                 peptidoglycan-binding 
                 
                   Lysobacter enzymogenes 
                 
                 763 
                 2615 
               
               
                   
                 protein 
               
               
                 ZLFS19 
                 lipoprotein nlpd/lppb 
                 
                   Lysobacter gummosus 
                 
                 764 
                 2616 
               
               
                   
                 homolog 
               
               
                 ZLFS21 
                 chitosanase 
                   Paenibacillus  spp. 
                 765 
                 2617 
               
               
                 ZLFS22 
                 Hypothetical protein 
                   Paenibacillus  spp. 
                 766 
                 2618 
               
               
                 ZLFS23 
                 beta-glucanase 
                   Pantoea  spp. 
                 767 
                 2619 
               
               
                 ZLFS24 
                 lipase 1 
                   Pantoea  spp. 
                 768 
                 2620 
               
               
                 ZLFS25 
                 SGNH hydrolase-type 
                   Pantoea  spp. 
                 769 
                 2621 
               
               
                   
                 esterase domain 
               
               
                 ZLFS27 
                 lytic transglycosylase 
                   Pseudomonas _In5 
                 770 
                 2622 
               
               
                 ZLFS28 
                 hydrolase 
                   Pseudomonas _In5 
                 771 
                 2623 
               
               
                 ZLFS29 
                 Beta-barrel assembly- 
                   Pseudomonas _In5 
                 772 
                 2624 
               
               
                   
                 enhancing protease 
               
               
                   
                 [bepA]. 
               
               
                 ZLFS30 
                 dipeptidase 
                   Pseudomonas _In5 
                 773 
                 2625 
               
               
                 ZLFS31 
                 lipase 1 
                   Serratia   —   plymuthica _S13 
                 774 
                 2626 
               
               
                 ZLFS32 
                 endoglucanase 
                   Serratia   —   plymuthica _S13 
                 775 
                 2627 
               
               
                 ZLFS33 
                 chitin-binding protein 
                   Serratia   —   plymuthica _S13 
                 776 
                 2628 
               
               
                 ZLFS34 
                 peptidyl-dipeptidase 
                   Serratia   —   plymuthica _S13 
                 777 
                 2629 
               
               
                   
                 dcp 
               
               
                 ZLFS35 
                 Hypothetical protein 
                   Serratia   —   plymuthica _S13 
                 778 
                 2630 
               
               
                 ZLFS36 
                 Calcium-mediated 
                   Serratia   —   plymuthica _S13 
                 779 
                 2631 
               
               
                   
                 lectin 
               
               
                 ZLFS37 
                 upf0339 protein yegp 
                   Serratia   —   plymuthica _S13 
                 780 
                 2632 
               
               
                 ZLFS38 
                 bacterioferritin 
                   Serratia   —   plymuthica _S13 
                 781 
                 2633 
               
               
                 ZLFS39 
                 Glycogen debranching 
                   Serratia   —   plymuthica _S13 
                 782 
                 2634 
               
               
                   
                 enzyme [glgX]. 
               
               
                 ZLFS42 
                 killer toxin like 
                   Streptomyces _HPH0547 
                 783 
                 2635 
               
               
                 ZLFS44 
                 glycoside hydrolase 
                   Streptomyces _M10 
                 784 
                 2636 
               
               
                 ZLFS45 
                 Oxidoreductase 
                   Streptomyces _M10 
                 785 
                 2637 
               
               
                 ZLFS47 
                 Antimicrobial protein 
                   Streptomyces   —   scopuliridis _RB72 
                 786 
                 2638 
               
               
                   
                 MiAMP1 
               
               
                 ZLFS48 
                 Antimicrobial protein 
                 
                   Streptosporangium 
                   — 
                   amethystogenes 
                 
                 787 
                 2639 
               
               
                   
                 MiAMP1 
               
               
                 ZLFS49 
                 Hypothetical protein 
                   Curtobacterium  spp. 
                 788 
                 2640 
               
               
                 ZLFS51 
                 Glycosyl hydrolase 
                   Bacillus   —   subtilis   —   subtilis _OH_131_1 
                 789 
                 2641 
               
               
                   
               
               
                 “polyn.” = polynucleotide; “polyp.” = polypeptide. 
               
            
           
         
       
     
     Example 12: Identification of Orthologous Sequences of Antifungal Proteins 
     Orthologues and paralogues constitute two major types of homologues: The first evolved from a common ancestor by specialization, and the latter are related by duplication events. It is assumed that paralogues arising from ancient duplication events are likely to have diverged in function while true orthologues are more likely to retain identical function over evolutionary time. Orthologues of the discovered antifungal genes are not only likely to be antifungal by themselves but also may hold improved potency or target different fungi spectra. 
     The search and identification of homologous genes involves the screening of sequence information available, for example, public databases such as the GenBank, and the European Molecular Biology Laboratory Nucleic Acid Sequence Database (EMBL). 
     Polynucleotides and polypeptides with significant homology to the identified genes described in Table 15 (Example 11) were identified from the databases using BLAST™ software with the Blastp and tBlastn algorithms as filters for the first stage, and the needle (EMBOSS package) or Frame+ algorithm alignment for the second stage. Local identity (BLAST™ alignments) was defined with a very permissive cutoff—40% Identity on a span of 40% of the sequences lengths because it is used only as a filter for the global alignment stage. The default filtering of the BLAST™ package was not utilized (by setting the parameter “-F F”). 
     In the second stage, homologs were defined based on a global identity of at least 70% to the core gene polypeptide sequence. Two distinct forms for finding the optimal global alignment for protein or nucleotide sequences were used in this application: 
     1. Between two proteins (following the BLASTP filter): 
     EMBOSS-6.0.1 Needleman-Wunsch algorithm with the following modified parameters: gapopen=8 gapextend=2. The rest of the parameters were unchanged from the default options described hereinabove. 
     2. Between a protein sequence and a nucleotide sequence (following the TBLASTN filter): GenCore 6.0 OneModel application utilizing the Frame+ algorithm with the following parameters: model=frame+_p2n.model mode=qglobal -q=protein. sequence -db=nucleotide. sequence. The rest of the parameters are unchanged from the default options described hereinabove. 
     Homology was calculated as % of identity over the aligned sequences. The query sequences were the polypeptide sequences depicted in Table 15 (Example 11). The subject sequences are protein sequences identified in the database based on greater than 80% global identity to the predicted translated sequences of the query nucleotide sequences or to the polypeptide sequences. 
     The identified orthologous and homologous sequences having at least 70% global sequence identity to the sequences are provided in Table 16, below. 
     
       
         
           
               
             
               
                 TABLE 16 
               
             
            
               
                   
               
               
                 Homologues (e.g., orthologues) of the identified antifungal genes/polypeptides 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Polyn. 
                 Hom. to 
                   
                 Polyp. 
                 Hom. to 
                 % 
                   
               
               
                 SEQ ID 
                 Gene 
                   
                 SEQ ID 
                 SEQ ID 
                 glob. 
               
               
                 NO 
                 Name 
                 Organism 
                 NO 
                 NO 
                 Iden. 
                 Algor. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 1187 
                 BLFS87 
                   Paenibacilhis  sp. 
                 3024 
                 2523 
                 96.2 
                 globlastp 
               
               
                 1188 
                 BLFS87 
                   Paenibacilhis  sp. 
                 3025 
                 2523 
                 95.5 
                 globlastp 
               
               
                 1189 
                 BLFS87 
                   Paenibacilhis  sp. 
                 3026 
                 2523 
                 94.8 
                 globlastp 
               
               
                 1190 
                 BLFS87 
                   Paenibacillus  sp. 
                 3027 
                 2523 
                 94.1 
                 globlastp 
               
               
                 1191 
                 BLFS87 
                   Paenibacillus  sp. 
                 3028 
                 2523 
                 93.5 
                 globlastp 
               
               
                 1192 
                 BLFS87 
                   Paenibacillus  sp. 
                 3029 
                 2523 
                 92 
                 globlastp 
               
               
                 1193 
                 BLFS87 
                   Paenibacillus  sp. 
                 3030 
                 2523 
                 91.1 
                 globlastp 
               
               
                 1194 
                 BLFS87 
                   Paenibacillus  sp. 
                 3031 
                 2523 
                 90.2 
                 globlastp 
               
               
                 1195 
                 BLFS87 
                   Paenibacillus  sp. 
                 3032 
                 2523 
                 89.4 
                 globlastp 
               
               
                 1196 
                 BLFS87 
                   Paenibacillus  sp. 
                 3033 
                 2523 
                 88.3 
                 globlastp 
               
               
                 1197 
                 BLFS87 
                   Paenibacillus  sp. 
                 3034 
                 2523 
                 71 
                 globlastp 
               
               
                 2052 
                 ZLFS3 
                   Bacillus  sp. 
                 3866 
                 2601 
                 98.5 
                 globlastp 
               
               
                 2053 
                 ZLFS3 
                   Bacillus  sp. 
                 3867 
                 2601 
                 97.8 
                 globlastp 
               
               
                 2054 
                 ZLFS3 
                   Bacillus  sp. 
                 3868 
                 2601 
                 97.4 
                 globlastp 
               
               
                 2055 
                 ZLFS3 
                   Bacillus  sp. 
                 3869 
                 2601 
                 94.9 
                 globlastp 
               
               
                 2056 
                 ZLFS3 
                   Bacillus  sp. 
                 3870 
                 2601 
                 94.6 
                 globlastp 
               
               
                 2057 
                 ZLFS3 
                   Bacillus  sp. 
                 3871 
                 2601 
                 94.2 
                 globlastp 
               
               
                 2058 
                 ZLFS3 
                   Bacillus  sp. 
                 3872 
                 2601 
                 93.8 
                 globlastp 
               
               
                 2059 
                 ZLFS3 
                   Bacillus  sp. 
                 3873 
                 2601 
                 84.1 
                 globlastp 
               
               
                 2060 
                 ZLFS3 
                   Bacillus  sp. 
                 3874 
                 2601 
                 83.8 
                 globlastp 
               
               
                 2061 
                 ZLFS3 
                   Bacillus  sp. 
                 3875 
                 2601 
                 76.1 
                 globlastp 
               
               
                 2062 
                 ZLFS3 
                   Bacillus  sp. 
                 3876 
                 2601 
                 75.7 
                 globlastp 
               
               
                 2063 
                 ZLFS3 
                   Bacillus  sp. 
                 3877 
                 2601 
                 75.5 
                 globlastp 
               
               
                 2064 
                 ZLFS3 
                   Bacillus  sp. 
                 3878 
                 2601 
                 75.4 
                 globlastp 
               
               
                 2065 
                 ZLFS3 
                   Bacillus  sp. 
                 3879 
                 2601 
                 75.2 
                 globlastp 
               
               
                 2066 
                 ZLFS3 
                   Bacillus  sp. 
                 3880 
                 2601 
                 74.9 
                 globlastp 
               
               
                 2067 
                 ZLFS3 
                   Bacillus  sp. 
                 3881 
                 2601 
                 74.5 
                 globlastp 
               
               
                 2068 
                 ZLFS3 
                   Bacillus  sp. 
                 3882 
                 2601 
                 74.1 
                 globlastp 
               
               
                 2069 
                 ZLFS3 
                   Bacillus  sp. 
                 3883 
                 2601 
                 73 
                 globlastp 
               
               
                 949 
                 BLFS34 
                   Bacillus  sp. 
                 2799 
                 2476 
                 99 
                 globlastp 
               
               
                 950 
                 BLFS34 
                   Bacillus  sp. 
                 2800 
                 2476 
                 98.1 
                 globlastp 
               
               
                 951 
                 BLFS34 
                   Bacillus  sp. 
                 2801 
                 2476 
                 95.1 
                 globlastp 
               
               
                 1013 
                 BLFS56 
                   Janthinobacterium  sp. 
                 2859 
                 2496 
                 98.7 
                 globlastp 
               
               
                 1014 
                 BLFS56 
                 Oxalobacteraceae sp. 
                 2860 
                 2496 
                 93.2 
                 globlastp 
               
               
                 1015 
                 BLFS56 
                   Janthinobacterium  sp. 
                 2861 
                 2496 
                 72.5 
                 globlastp 
               
               
                 1016 
                 BLFS56 
                   Janthinobacterium  sp. 
                 2862 
                 2496 
                 72.2 
                 globlastp 
               
               
                 1017 
                 BLFS56 
                   Janthinobacterium  sp. 
                 2863 
                 2496 
                 71.6 
                 globlastp 
               
               
                 1018 
                 BLFS56 
                   Massilia  sp. 
                 2864 
                 2496 
                 71.4 
                 globlastp 
               
               
                 1019 
                 BLFS56 
                   Massilia  sp. 
                 — 
                 2496 
                 71.02 
                 glotblastn 
               
               
                 1020 
                 BLFS56 
                   Massilia  sp. 
                 — 
                 2496 
                 70.7 
                 glotblastn 
               
               
                 1021 
                 BLFS56 
                   Duganella  sp. 
                 — 
                 2496 
                 70.04 
                 glotblastn 
               
               
                 1207 
                 BLFS89 
                   Paenibacillus  sp. 
                 3044 
                 2525 
                 99.2 
                 globlastp 
               
               
                 1208 
                 BLFS89 
                   Paenibacillus  sp. 
                 3045 
                 2525 
                 98.7 
                 globlastp 
               
               
                 1209 
                 BLFS89 
                   Paenibacillus  sp. 
                 3046 
                 2525 
                 97 
                 globlastp 
               
               
                 1210 
                 BLFS89 
                   Paenibacillus  sp. 
                 3047 
                 2525 
                 96.7 
                 globlastp 
               
               
                 1211 
                 BLFS89 
                   Paenibacillus  sp. 
                 3048 
                 2525 
                 95 
                 globlastp 
               
               
                 1212 
                 BLFS89 
                   Paenibacillus  sp. 
                 3049 
                 2525 
                 94.2 
                 globlastp 
               
               
                 1213 
                 BLFS89 
                   Paenibacillus  sp. 
                 3050 
                 2525 
                 93.4 
                 globlastp 
               
               
                 1214 
                 BLFS89 
                   Paenibacillus  sp. 
                 3051 
                 2525 
                 92.2 
                 globlastp 
               
               
                 1215 
                 BLFS89 
                   Paenibacillus  sp. 
                 3052 
                 2525 
                 91.9 
                 globlastp 
               
               
                 1216 
                 BLFS89 
                   Paenibacillus  sp. 
                 — 
                 2525 
                 71.86 
                 glotblastn 
               
               
                 1217 
                 BLFS89 
                   Paenibacillus  sp. 
                 — 
                 2525 
                 70.07 
                 glotblastn 
               
               
                 884 
                 BLFS23 
                   Bacillus  sp. 
                 2734 
                 2466 
                 96.4 
                 globlastp 
               
               
                 885 
                 BLFS23 
                   Bacillus  sp. 
                 2735 
                 2466 
                 95.2 
                 globlastp 
               
               
                 886 
                 BLFS23 
                   Bacillus  sp. 
                 2736 
                 2466 
                 94 
                 globlastp 
               
               
                 887 
                 BLFS23 
                   Bacillus  sp. 
                 2737 
                 2466 
                 93.1 
                 globlastp 
               
               
                 888 
                 BLFS23 
                   Bacillus  sp. 
                 2738 
                 2466 
                 93 
                 globlastp 
               
               
                 889 
                 BLFS23 
                   Bacillus  sp. 
                 2739 
                 2466 
                 92.4 
                 globlastp 
               
               
                 890 
                 BLFS23 
                   Bacillus  sp. 
                 2740 
                 2466 
                 92 
                 globlastp 
               
               
                 891 
                 BLFS23 
                   Bacillus  sp. 
                 2741 
                 2466 
                 91.8 
                 globlastp 
               
               
                 892 
                 BLFS23 
                   Bacillus  sp. 
                 2742 
                 2466 
                 91.2 
                 globlastp 
               
               
                 1062 
                 BLFS64 
                   Geobacillus  sp. 
                 2904 
                 2504 
                 98.8 
                 globlastp 
               
               
                 2194 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4008 
                 2623 
                 94.1 
                 globlastp 
               
               
                 2195 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4009 
                 2623 
                 93.8 
                 globlastp 
               
               
                 2196 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4010 
                 2623 
                 92.2 
                 globlastp 
               
               
                 2197 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4011 
                 2623 
                 91.4 
                 globlastp 
               
               
                 2198 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4012 
                 2623 
                 89.5 
                 globlastp 
               
               
                 2199 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4013 
                 2623 
                 88.3 
                 globlastp 
               
               
                 2200 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4014 
                 2623 
                 87.9 
                 globlastp 
               
               
                 2201 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4015 
                 2623 
                 87.5 
                 globlastp 
               
               
                 2202 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4016 
                 2623 
                 86.7 
                 globlastp 
               
               
                 2203 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4017 
                 2623 
                 86.3 
                 globlastp 
               
               
                 2204 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4018 
                 2623 
                 85.9 
                 globlastp 
               
               
                 2205 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4019 
                 2623 
                 85.5 
                 globlastp 
               
               
                 2206 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4020 
                 2623 
                 82.7 
                 globlastp 
               
               
                 2207 
                 ZLFS28 
                   Pseudomonas  sp. 
                 4021 
                 2623 
                 80.1 
                 globlastp 
               
               
                 2208 
                 ZLFS28 
                   Collimonas  sp. 
                 4022 
                 2623 
                 72.1 
                 globlastp 
               
               
                 908 
                 BLFS29 
                   Bacillus  sp. 
                 2758 
                 2471 
                 75.3 
                 globlastp 
               
               
                 2104 
                 ZLFS19 
                 Environmental sample 
                 3918 
                 2616 
                 84.6 
                 globlastp 
               
               
                 2105 
                 ZLFS19 
                   Lysobacter  sp. 
                 3919 
                 2616 
                 84.3 
                 globlastp 
               
               
                 2106 
                 ZLFS19 
                   Lysobacter  sp. 
                 3920 
                 2616 
                 83.9 
                 globlastp 
               
               
                 2107 
                 ZLFS19 
                   Lysobacter  sp. 
                 3921 
                 2616 
                 83.4 
                 globlastp 
               
               
                 2108 
                 ZLFS19 
                   Lysobacter  sp. 
                 3922 
                 2616 
                 82.4 
                 globlastp 
               
               
                 2109 
                 ZLFS19 
                 Environmental sample 
                 3923 
                 2616 
                 81.9 
                 globlastp 
               
               
                 2110 
                 ZLFS19 
                 Environmental sample 
                 3924 
                 2616 
                 81.5 
                 globlastp 
               
               
                 2111 
                 ZLFS19 
                 Environmental sample 
                 3925 
                 2616 
                 80.6 
                 globlastp 
               
               
                 2112 
                 ZLFS19 
                   Lysobacter  sp. 
                 3926 
                 2616 
                 79.7 
                 globlastp 
               
               
                 2113 
                 ZLFS19 
                   Lysobacter  sp. 
                 3927 
                 2616 
                 76.9 
                 globlastp 
               
               
                 845 
                 BLFS18 
                   Bacillus  sp. 
                 2697 
                 2461 
                 95.5 
                 globlastp 
               
               
                 846 
                 BLFS18 
                   Bacillus  sp. 
                 2698 
                 2461 
                 84.6 
                 globlastp 
               
               
                 847 
                 BLFS18 
                   Bacillus  sp. 
                 2699 
                 2461 
                 82.1 
                 globlastp 
               
               
                 848 
                 BLFS18 
                   Bacillus  sp. 
                 2700 
                 2461 
                 81.9 
                 globlastp 
               
               
                 849 
                 BLFS18 
                   Bacillus  sp. 
                 2701 
                 2461 
                 80.1 
                 globlastp 
               
               
                 850 
                 BLFS18 
                   Bacillus  sp. 
                 2702 
                 2461 
                 78.1 
                 globlastp 
               
               
                 851 
                 BLFS18 
                   Bacillus  sp. 
                 2703 
                 2461 
                 77 
                 globlastp 
               
               
                 852 
                 BLFS18 
                   Bacillus  sp. 
                 2704 
                 2461 
                 76.3 
                 globlastp 
               
               
                 853 
                 BLFS18 
                   Bacillus  sp. 
                 2705 
                 2461 
                 75 
                 globlastp 
               
               
                 854 
                 BLFS18 
                   Bacillus  sp. 
                 2706 
                 2461 
                 74.2 
                 globlastp 
               
               
                 855 
                 BLFS18 
                   Bacillus  sp. 
                 2707 
                 2461 
                 73 
                 globlastp 
               
               
                 856 
                 BLFS18 
                   Kurthia  sp. 
                 2708 
                 2461 
                 72 
                 globlastp 
               
               
                 857 
                 BLFS18 
                   Bacillus  sp. 
                 2709 
                 2461 
                 71 
                 globlastp 
               
               
                 858 
                 BLFS18 
                   Anoxybacillus  sp. 
                 2710 
                 2461 
                 70.3 
                 globlastp 
               
               
                 1997 
                 BLFS155 
                   Streptomyces  sp. 
                 3811 
                 2590 
                 88.1 
                 globlastp 
               
               
                 1998 
                 BLFS155 
                   Streptomyces  sp. 
                 3812 
                 2590 
                 70.2 
                 globlastp 
               
               
                 592 
                 BLFS156 
                   Streptomyces  sp. 
                 — 
                 2591 
                 71.07 
                 glotblastn 
               
               
                 1696 
                 BLFS130 
                   Pseudomonas  sp. 
                 3520 
                 2565 
                 98.8 
                 globlastp 
               
               
                 1697 
                 BLFS130 
                   Pseudomonas  sp. 
                 3521 
                 2565 
                 93.9 
                 globlastp 
               
               
                 1698 
                 BLFS130 
                   Pseudomonas  sp. 
                 3522 
                 2565 
                 81.2 
                 globlastp 
               
               
                 1699 
                 BLFS130 
                   Pseudomonas  sp. 
                 3523 
                 2565 
                 72.9 
                 globlastp 
               
               
                 1700 
                 BLFS130 
                   Pseudomonas  sp. 
                 3524 
                 2565 
                 70 
                 globlastp 
               
               
                 2426 
                 ZLFS51 
                   Bacillus  sp. 
                 4235 
                 2641 
                 99.1 
                 globlastp 
               
               
                 2427 
                 ZLFS51 
                   Bacillus  sp. 
                 4236 
                 2641 
                 98.1 
                 globlastp 
               
               
                 2428 
                 ZLFS51 
                   Bacillus  sp. 
                 4237 
                 2641 
                 97.2 
                 globlastp 
               
               
                 2429 
                 ZLFS51 
                   Bacillus  sp. 
                 4238 
                 2641 
                 96 
                 globlastp 
               
               
                 2430 
                 ZLFS51 
                   Salinibacillus  sp. 
                 4239 
                 2641 
                 95.3 
                 globlastp 
               
               
                 2431 
                 ZLFS51 
                   Bacillus  sp. 
                 4240 
                 2641 
                 94.6 
                 globlastp 
               
               
                 2432 
                 ZLFS51 
                   Bacillus  sp. 
                 4241 
                 2641 
                 93.9 
                 globlastp 
               
               
                 2433 
                 ZLFS51 
                   Bacillus  sp. 
                 4242 
                 2641 
                 87.6 
                 globlastp 
               
               
                 2434 
                 ZLFS51 
                   Bacillus  sp. 
                 4243 
                 2641 
                 86.2 
                 globlastp 
               
               
                 2435 
                 ZLFS51 
                   Bacillus  sp. 
                 4244 
                 2641 
                 85.7 
                 globlastp 
               
               
                 2436 
                 ZLFS51 
                   Bacillus  sp. 
                 4245 
                 2641 
                 78 
                 globlastp 
               
               
                 2437 
                 ZLFS51 
                   Bacillus  sp. 
                 4246 
                 2641 
                 77.1 
                 globlastp 
               
               
                 2438 
                 ZLFS51 
                   Bacillus  sp. 
                 4247 
                 2641 
                 76 
                 globlastp 
               
               
                 2439 
                 ZLFS51 
                   Bacillus  sp. 
                 4248 
                 2641 
                 75.2 
                 globlastp 
               
               
                 2440 
                 ZLFS51 
                   Bacillus  sp. 
                 4249 
                 2641 
                 74 
                 globlastp 
               
               
                 2441 
                 ZLFS51 
                   Bacillus  sp. 
                 4250 
                 2641 
                 73.8 
                 globlastp 
               
               
                 2442 
                 ZLFS51 
                   Bacillus  sp. 
                 4251 
                 2641 
                 71.1 
                 globlastp 
               
               
                 2443 
                 ZLFS51 
                   Bacillus  sp. 
                 4252 
                 2641 
                 70 
                 globlastp 
               
               
                 2389 
                 ZLFS42 
                   Streptomyces  sp. 
                 4202 
                 2635 
                 80.3 
                 globlastp 
               
               
                 2390 
                 ZLFS42 
                   Streptomyces  sp. 
                 4203 
                 2635 
                 78.6 
                 globlastp 
               
               
                 2391 
                 ZLFS42 
                   Streptomyces  sp. 
                 4204 
                 2635 
                 78.6 
                 globlastp 
               
               
                 2392 
                 ZLFS42 
                   Streptomyces  sp. 
                 4205 
                 2635 
                 77.8 
                 globlastp 
               
               
                 785 
                 ZLFS42 
                   Streptomyces  sp. 
                 2637 
                 2635 
                 77.8 
                 globlastp 
               
               
                 2393 
                 ZLFS42 
                   Streptomyces  sp. 
                 4206 
                 2635 
                 76.9 
                 globlastp 
               
               
                 2394 
                 ZLFS42 
                   Streptomyces  sp. 
                 4207 
                 2635 
                 76.1 
                 globlastp 
               
               
                 2395 
                 ZLFS42 
                   Streptomyces  sp. 
                 4207 
                 2635 
                 76.1 
                 globlastp 
               
               
                 2396 
                 ZLFS42 
                   Streptomyces  sp. 
                 4208 
                 2635 
                 75.2 
                 globlastp 
               
               
                 2397 
                 ZLFS42 
                   Streptomyces  sp. 
                 4209 
                 2635 
                 73.5 
                 globlastp 
               
               
                 2398 
                 ZLFS42 
                   Streptomyces  sp. 
                 4210 
                 2635 
                 73.5 
                 globlastp 
               
               
                 808 
                 ZLFS42 
                   Streptomyces  sp. 
                 2660 
                 2635 
                 70.1 
                 globlastp 
               
               
                 1792 
                 BLFS136 
                   Pseudoxanthomonas  sp. 
                 3616 
                 2571 
                 81 
                 globlastp 
               
               
                 1163 
                 BLFS84 
                   Paenibacillus  sp. 
                 3000 
                 2520 
                 98.3 
                 globlastp 
               
               
                 1164 
                 BLFS84 
                   Paenibacillus  sp. 
                 3001 
                 2520 
                 95.9 
                 globlastp 
               
               
                 1165 
                 BLFS84 
                   Paenibacillus  sp. 
                 3002 
                 2520 
                 95.1 
                 globlastp 
               
               
                 1166 
                 BLFS84 
                   Paenibacillus  sp. 
                 3003 
                 2520 
                 92.4 
                 globlastp 
               
               
                 1167 
                 BLFS84 
                   Paenibacillus  sp. 
                 3004 
                 2520 
                 89.5 
                 globlastp 
               
               
                 1168 
                 BLFS84 
                   Paenibacillus  sp. 
                 3005 
                 2520 
                 88.1 
                 globlastp 
               
               
                 1169 
                 BLFS84 
                   Paenibacillus  sp. 
                 3006 
                 2520 
                 87.5 
                 globlastp 
               
               
                 1129 
                 BLFS79 
                   Paenibacillus  sp. 
                 2966 
                 2516 
                 99.2 
                 globlastp 
               
               
                 1130 
                 BLFS79 
                   Paenibacillus  sp. 
                 2967 
                 2516 
                 98.5 
                 globlastp 
               
               
                 1131 
                 BLFS79 
                   Paenibacillus  sp. 
                 2968 
                 2516 
                 97 
                 globlastp 
               
               
                 1132 
                 BLFS79 
                   Paenibacillus  sp. 
                 2969 
                 2516 
                 96.6 
                 globlastp 
               
               
                 1133 
                 BLFS79 
                   Paenibacillus  sp. 
                 2970 
                 2516 
                 95.1 
                 globlastp 
               
               
                 1134 
                 BLFS79 
                   Paenibacillus  sp. 
                 2971 
                 2516 
                 94 
                 globlastp 
               
               
                 1135 
                 BLFS79 
                   Paenibacillus  sp. 
                 2972 
                 2516 
                 93.6 
                 globlastp 
               
               
                 1136 
                 BLFS79 
                   Paenibacillus  sp. 
                 2973 
                 2516 
                 93.2 
                 globlastp 
               
               
                 1137 
                 BLFS79 
                   Paenibacillus  sp. 
                 2974 
                 2516 
                 92.5 
                 globlastp 
               
               
                 1138 
                 BLFS79 
                   Paenibacillus  sp. 
                 2975 
                 2516 
                 91.7 
                 globlastp 
               
               
                 2235 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4049 
                 2625 
                 94.6 
                 globlastp 
               
               
                 2236 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4050 
                 2625 
                 90.5 
                 globlastp 
               
               
                 2237 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4051 
                 2625 
                 89.1 
                 globlastp 
               
               
                 2238 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4052 
                 2625 
                 88.3 
                 globlastp 
               
               
                 2239 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4053 
                 2625 
                 87.2 
                 globlastp 
               
               
                 2240 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4054 
                 2625 
                 86 
                 globlastp 
               
               
                 2241 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4055 
                 2625 
                 85 
                 globlastp 
               
               
                 2242 
                 ZLFS30 
                   Erwinia  sp. 
                 4056 
                 2625 
                 84.1 
                 globlastp 
               
               
                 2243 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4057 
                 2625 
                 83.1 
                 globlastp 
               
               
                 2244 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4058 
                 2625 
                 82 
                 globlastp 
               
               
                 2245 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4059 
                 2625 
                 81 
                 globlastp 
               
               
                 2246 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4060 
                 2625 
                 80.1 
                 globlastp 
               
               
                 2247 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4061 
                 2625 
                 79.1 
                 globlastp 
               
               
                 2248 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4062 
                 2625 
                 78.1 
                 globlastp 
               
               
                 2249 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4063 
                 2625 
                 77.2 
                 globlastp 
               
               
                 2250 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4064 
                 2625 
                 76.7 
                 globlastp 
               
               
                 2251 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4065 
                 2625 
                 75.5 
                 globlastp 
               
               
                 2252 
                 ZLFS30 
                 Environmental sample 
                 4066 
                 2625 
                 72 
                 globlastp 
               
               
                 2253 
                 ZLFS30 
                   Pseudomonas  sp. 
                 4067 
                 2625 
                 71 
                 globlastp 
               
               
                 2254 
                 ZLFS30 
                 Environmental sample 
                 4068 
                 2625 
                 70.1 
                 globlastp 
               
               
                 2033 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3847 
                 2600 
                 99.4 
                 globlastp 
               
               
                 2034 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3848 
                 2600 
                 96.3 
                 globlastp 
               
               
                 2035 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3849 
                 2600 
                 94.6 
                 globlastp 
               
               
                 2036 
                 ZLFS2 
                 Environmental sample 
                 3850 
                 2600 
                 92.7 
                 globlastp 
               
               
                 2037 
                 ZLFS2 
                 Environmental sample 
                 3851 
                 2600 
                 92.1 
                 globlastp 
               
               
                 2038 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3852 
                 2600 
                 90.1 
                 globlastp 
               
               
                 2039 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3853 
                 2600 
                 89.8 
                 globlastp 
               
               
                 2040 
                 ZLFS2 
                 Environmental sample 
                 3854 
                 2600 
                 89.3 
                 globlastp 
               
               
                 2041 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3855 
                 2600 
                 88.9 
                 globlastp 
               
               
                 2042 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3856 
                 2600 
                 87.3 
                 globlastp 
               
               
                 2043 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3857 
                 2600 
                 86.2 
                 globlastp 
               
               
                 2044 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3858 
                 2600 
                 73.7 
                 globlastp 
               
               
                 2045 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3859 
                 2600 
                 73.2 
                 globlastp 
               
               
                 2046 
                 ZLFS2 
                   Sporosarcina  sp. 
                 3860 
                 2600 
                 72.6 
                 globlastp 
               
               
                 2047 
                 ZLFS2 
                   Bhargavaea  sp. 
                 3861 
                 2600 
                 72.1 
                 globlastp 
               
               
                 2048 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3862 
                 2600 
                 71.5 
                 globlastp 
               
               
                 2049 
                 ZLFS2 
                   Arthrobacter  sp. 
                 3863 
                 2600 
                 71.2 
                 globlastp 
               
               
                 2050 
                 ZLFS2 
                   Sporosarcina  sp. 
                 3864 
                 2600 
                 70.6 
                 globlastp 
               
               
                 2051 
                 ZLFS2 
                   Planomicrobium  sp. 
                 3865 
                 2600 
                 70.5 
                 globlastp 
               
               
                 1071 
                 BLFS72 
                   Lysobacter  sp. 
                 2913 
                 2510 
                 95 
                 globlastp 
               
               
                 1124 
                 BLFS77 
                   Paenibacillus  sp. 
                 2963 
                 2515 
                 98.1 
                 globlastp 
               
               
                 1125 
                 BLFS77 
                   Paenibacillus  sp. 
                 2964 
                 2515 
                 96.6 
                 globlastp 
               
               
                 1126 
                 BLFS77 
                   Paenibacillus  sp. 
                 2965 
                 2515 
                 81.1 
                 globlastp 
               
               
                 1127 
                 BLFS77 
                   Paenibacillus  sp. 
                 — 
                 2515 
                 73.95 
                 glotblastn 
               
               
                 1128 
                 BLFS77 
                   Paenibacillus  sp. 
                 — 
                 2515 
                 73.24 
                 glotblastn 
               
               
                 1599 
                 BLFS122 
                   Pseudomonas  sp. 
                 3424 
                 2557 
                 97.7 
                 globlastp 
               
               
                 1600 
                 BLFS122 
                   Pseudomonas  sp. 
                 3425 
                 2557 
                 96.9 
                 globlastp 
               
               
                 1601 
                 BLFS122 
                   Pseudomonas  sp. 
                 3426 
                 2557 
                 96.2 
                 globlastp 
               
               
                 1602 
                 BLFS122 
                   Pseudomonas  sp. 
                 3427 
                 2557 
                 93.1 
                 globlastp 
               
               
                 1603 
                 BLFS122 
                   Pseudomonas  sp. 
                 3428 
                 2557 
                 79.4 
                 globlastp 
               
               
                 1604 
                 BLFS122 
                   Pseudomonas  sp. 
                 — 
                 2557 
                 72.18 
                 glotblastn 
               
               
                 1605 
                 BLFS122 
                   Pseudomonas  sp. 
                 3429 
                 2557 
                 71.1 
                 globlastp 
               
               
                 1606 
                 BLFS122 
                   Pseudomonas  sp. 
                 3430 
                 2557 
                 70.9 
                 globlastp 
               
               
                 970 
                 BLFS38 
                   Brevibacillus  sp. 
                 2818 
                 2480 
                 81.2 
                 globlastp 
               
               
                 1139 
                 BLFS82 
                   Paenibacillus  sp. 
                 2976 
                 2518 
                 99 
                 globlastp 
               
               
                 1140 
                 BLFS82 
                   Paenibacillus  sp. 
                 2977 
                 2518 
                 98 
                 globlastp 
               
               
                 1141 
                 BLFS82 
                   Paenibacillus  sp. 
                 2978 
                 2518 
                 97.1 
                 globlastp 
               
               
                 1142 
                 BLFS82 
                   Paenibacillus  sp. 
                 2979 
                 2518 
                 96.1 
                 globlastp 
               
               
                 1143 
                 BLFS82 
                   Paenibacillus  sp. 
                 2980 
                 2518 
                 94.1 
                 globlastp 
               
               
                 1144 
                 BLFS82 
                   Paenibacillus  sp. 
                 2981 
                 2518 
                 93.1 
                 globlastp 
               
               
                 1145 
                 BLFS82 
                   Paenibacillus  sp. 
                 2982 
                 2518 
                 92.2 
                 globlastp 
               
               
                 1146 
                 BLFS82 
                   Paenibacillus  sp. 
                 2983 
                 2518 
                 89.4 
                 globlastp 
               
               
                 1147 
                 BLFS82 
                   Paenibacillus  sp. 
                 2984 
                 2518 
                 88.2 
                 globlastp 
               
               
                 1148 
                 BLFS82 
                   Paenibacillus  sp. 
                 2985 
                 2518 
                 86.3 
                 globlastp 
               
               
                 1149 
                 BLFS82 
                   Paenibacillus  sp. 
                 2986 
                 2518 
                 85.3 
                 globlastp 
               
               
                 1150 
                 BLFS82 
                   Paenibacillus  sp. 
                 2987 
                 2518 
                 84.3 
                 globlastp 
               
               
                 1151 
                 BLFS82 
                   Paenibacillus  sp. 
                 2988 
                 2518 
                 82.4 
                 globlastp 
               
               
                 2075 
                 ZLFS6 
                   Collimonas  sp. 
                 3889 
                 2603 
                 95.7 
                 globlastp 
               
               
                 2076 
                 ZLFS6 
                   Herbaspirillum  sp. 
                 3890 
                 2603 
                 72 
                 globlastp 
               
               
                 2077 
                 ZLFS6 
                   Janthinobacterium  sp. 
                 3891 
                 2603 
                 71.3 
                 globlastp 
               
               
                 2078 
                 ZLFS6 
                   Janthinobacterium  sp. 
                 3892 
                 2603 
                 70.7 
                 globlastp 
               
               
                 2079 
                 ZLFS6 
                 Oxalobacteraceae sp. 
                 3893 
                 2603 
                 70.6 
                 globlastp 
               
               
                 812 
                 BLFS11 
                   Arthrobacter  sp. 
                 2664 
                 2454 
                 96.3 
                 globlastp 
               
               
                 813 
                 BLFS11 
                   Arthrobacter  sp. 
                 2665 
                 2454 
                 80.4 
                 globlastp 
               
               
                 814 
                 BLFS11 
                   Arthrobacter  sp. 
                 2666 
                 2454 
                 79.9 
                 globlastp 
               
               
                 815 
                 BLFS11 
                 Environmental sample 
                 2667 
                 2454 
                 75.7 
                 globlastp 
               
               
                 816 
                 BLFS11 
                   Arthrobacter  sp. 
                 2668 
                 2454 
                 73 
                 globlastp 
               
               
                 817 
                 BLFS11 
                   Arthrobacter  sp. 
                 2669 
                 2454 
                 71.2 
                 globlastp 
               
               
                 818 
                 BLFS11 
                   Arthrobacter  sp. 
                 2670 
                 2454 
                 70.1 
                 globlastp 
               
               
                 2114 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3928 
                 2617 
                 99.1 
                 globlastp 
               
               
                 2115 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3929 
                 2617 
                 96.7 
                 globlastp 
               
               
                 2116 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3930 
                 2617 
                 96.4 
                 globlastp 
               
               
                 2117 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3931 
                 2617 
                 94.5 
                 globlastp 
               
               
                 2118 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3932 
                 2617 
                 94.2 
                 globlastp 
               
               
                 2119 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3933 
                 2617 
                 91.3 
                 globlastp 
               
               
                 2120 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3934 
                 2617 
                 85 
                 globlastp 
               
               
                 2121 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3935 
                 2617 
                 84.5 
                 globlastp 
               
               
                 2122 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3936 
                 2617 
                 84.3 
                 globlastp 
               
               
                 2123 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3937 
                 2617 
                 82.6 
                 globlastp 
               
               
                 2124 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3938 
                 2617 
                 81.9 
                 globlastp 
               
               
                 2125 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3939 
                 2617 
                 81.6 
                 globlastp 
               
               
                 2126 
                 ZLFS21 
                   Paenibacillus  sp. 
                 3940 
                 2617 
                 72.3 
                 globlastp 
               
               
                 839 
                 BLFS17 
                 Multievos46 sp. 
                 2691 
                 2460 
                 96 
                 globlastp 
               
               
                 840 
                 BLFS17 
                 Environmental sample 
                 2692 
                 2460 
                 86.4 
                 globlastp 
               
               
                 841 
                 BLFS17 
                   Arthrobacter  sp. 
                 2693 
                 2460 
                 80 
                 globlastp 
               
               
                 842 
                 BLFS17 
                   Arthrobacter  sp. 
                 2694 
                 2460 
                 79 
                 globlastp 
               
               
                 843 
                 BLFS17 
                   Arthrobacter  sp. 
                 2695 
                 2460 
                 78.6 
                 globlastp 
               
               
                 844 
                 BLFS17 
                   Arthrobacter  sp. 
                 2696 
                 2460 
                 77.5 
                 globlastp 
               
               
                 1450 
                 BLFS113 
                   Pseudomonas  sp. 
                 3276 
                 2549 
                 99.4 
                 globlastp 
               
               
                 1451 
                 BLFS113 
                   Pseudomonas  sp. 
                 3277 
                 2549 
                 98.8 
                 globlastp 
               
               
                 1452 
                 BLFS113 
                   Pseudomonas  sp. 
                 3278 
                 2549 
                 97.6 
                 globlastp 
               
               
                 1453 
                 BLFS113 
                   Pseudomonas  sp. 
                 3279 
                 2549 
                 97 
                 globlastp 
               
               
                 1454 
                 BLFS113 
                   Pseudomonas  sp. 
                 3280 
                 2549 
                 96.4 
                 globlastp 
               
               
                 1455 
                 BLFS113 
                   Pseudomonas  sp. 
                 3281 
                 2549 
                 94.6 
                 globlastp 
               
               
                 1456 
                 BLFS113 
                   Pseudomonas  sp. 
                 3282 
                 2549 
                 92.2 
                 globlastp 
               
               
                 1457 
                 BLFS113 
                   Pseudomonas  sp. 
                 3283 
                 2549 
                 91 
                 globlastp 
               
               
                 1458 
                 BLFS113 
                   Pseudomonas  sp. 
                 3284 
                 2549 
                 90.4 
                 globlastp 
               
               
                 1459 
                 BLFS113 
                   Pseudomonas  sp. 
                 3285 
                 2549 
                 89.2 
                 globlastp 
               
               
                 1460 
                 BLFS113 
                   Pseudomonas  sp. 
                 3286 
                 2549 
                 88 
                 globlastp 
               
               
                 1461 
                 BLFS113 
                   Erwinia  sp. 
                 3287 
                 2549 
                 87.4 
                 globlastp 
               
               
                 1462 
                 BLFS113 
                   Pseudomonas  sp. 
                 3288 
                 2549 
                 86.2 
                 globlastp 
               
               
                 1463 
                 BLFS113 
                   Pseudomonas  sp. 
                 3289 
                 2549 
                 85 
                 globlastp 
               
               
                 1464 
                 BLFS113 
                   Pseudomonas  sp. 
                 3290 
                 2549 
                 84.2 
                 globlastp 
               
               
                 1465 
                 BLFS113 
                   Pseudomonas  sp. 
                 3291 
                 2549 
                 83 
                 globlastp 
               
               
                 1466 
                 BLFS113 
                   Pseudomonas  sp. 
                 3292 
                 2549 
                 82 
                 globlastp 
               
               
                 1467 
                 BLFS113 
                   Pseudomonas  sp. 
                 3293 
                 2549 
                 81.4 
                 globlastp 
               
               
                 1468 
                 BLFS113 
                   Pseudomonas  sp. 
                 3294 
                 2549 
                 80.1 
                 globlastp 
               
               
                 1469 
                 BLFS113 
                   Pseudomonas  sp. 
                 3295 
                 2549 
                 79 
                 globlastp 
               
               
                 1470 
                 BLFS113 
                   Pseudomonas  sp. 
                 3296 
                 2549 
                 78.4 
                 globlastp 
               
               
                 1471 
                 BLFS113 
                   Pseudomonas  sp. 
                 3297 
                 2549 
                 77.8 
                 globlastp 
               
               
                 1472 
                 BLFS113 
                 Environmental sample 
                 3298 
                 2549 
                 76.6 
                 globlastp 
               
               
                 1473 
                 BLFS113 
                   Pseudomonas  sp. 
                 3299 
                 2549 
                 74.9 
                 globlastp 
               
               
                 1474 
                 BLFS113 
                   Pseudomonas  sp. 
                 3300 
                 2549 
                 71.4 
                 globlastp 
               
               
                 817 
                 BLFS13 
                   Arthrobacter  sp. 
                 2669 
                 2456 
                 99.4 
                 globlastp 
               
               
                 815 
                 BLFS13 
                 Environmental sample 
                 2667 
                 2456 
                 73.3 
                 globlastp 
               
               
                 821 
                 BLFS13 
                   Arthrobacter  sp. 
                 2673 
                 2456 
                 71.4 
                 globlastp 
               
               
                 822 
                 BLFS13 
                   Arthrobacter  sp. 
                 2674 
                 2456 
                 70.3 
                 globlastp 
               
               
                 1611 
                 BLFS124 
                   Pseudomonas  sp. 
                 3435 
                 2559 
                 99 
                 globlastp 
               
               
                 1612 
                 BLFS124 
                   Pseudomonas  sp. 
                 3436 
                 2559 
                 93.3 
                 globlastp 
               
               
                 1613 
                 BLFS124 
                   Pseudomonas  sp. 
                 3437 
                 2559 
                 92.4 
                 globlastp 
               
               
                 1614 
                 BLFS124 
                   Pseudomonas  sp. 
                 3438 
                 2559 
                 83.8 
                 globlastp 
               
               
                 1615 
                 BLFS124 
                   Pseudomonas  sp. 
                 3439 
                 2559 
                 82.9 
                 globlastp 
               
               
                 1616 
                 BLFS124 
                   Pseudomonas  sp. 
                 3440 
                 2559 
                 81 
                 globlastp 
               
               
                 1617 
                 BLFS124 
                   Pseudomonas  sp. 
                 3441 
                 2559 
                 80 
                 globlastp 
               
               
                 1618 
                 BLFS124 
                   Pseudomonas  sp. 
                 3442 
                 2559 
                 79 
                 globlastp 
               
               
                 1619 
                 BLFS124 
                   Pseudomonas  sp. 
                 3443 
                 2559 
                 78.1 
                 globlastp 
               
               
                 1620 
                 BLFS124 
                   Pseudomonas  sp. 
                 3444 
                 2559 
                 77.3 
                 globlastp 
               
               
                 1621 
                 BLFS124 
                 Environmental sample 
                 3445 
                 2559 
                 76.2 
                 globlastp 
               
               
                 1622 
                 BLFS124 
                   Pseudomonas  sp. 
                 3446 
                 2559 
                 75.2 
                 globlastp 
               
               
                 1623 
                 BLFS124 
                   Pseudomonas  sp. 
                 3447 
                 2559 
                 74.5 
                 globlastp 
               
               
                 1624 
                 BLFS124 
                 Environmental sample 
                 3448 
                 2559 
                 73.3 
                 globlastp 
               
               
                 1625 
                 BLFS124 
                 Environmental sample 
                 3449 
                 2559 
                 72.4 
                 globlastp 
               
               
                 1626 
                 BLFS124 
                 Environmental sample 
                 3450 
                 2559 
                 71.4 
                 globlastp 
               
               
                 1627 
                 BLFS124 
                   Pseudomonas  sp. 
                 3451 
                 2559 
                 70.5 
                 globlastp 
               
               
                 2147 
                 ZLFS24 
                   Meta  sp. 
                 3961 
                 2620 
                 98.8 
                 globlastp 
               
               
                 2148 
                 ZLFS24 
                 Environmental sample 
                 3962 
                 2620 
                 98.6 
                 globlastp 
               
               
                 2149 
                 ZLFS24 
                 Environmental sample 
                 3963 
                 2620 
                 98.3 
                 globlastp 
               
               
                 2150 
                 ZLFS24 
                   Pantoea  sp. 
                 3964 
                 2620 
                 98.2 
                 globlastp 
               
               
                 2151 
                 ZLFS24 
                 Environmental sample 
                 3965 
                 2620 
                 98 
                 globlastp 
               
               
                 2152 
                 ZLFS24 
                 Environmental sample 
                 3966 
                 2620 
                 97.9 
                 globlastp 
               
               
                 2153 
                 ZLFS24 
                   Pantoea  sp. 
                 3967 
                 2620 
                 93.6 
                 globlastp 
               
               
                 2154 
                 ZLFS24 
                   Pantoea  sp. 
                 3968 
                 2620 
                 93.3 
                 globlastp 
               
               
                 2155 
                 ZLFS24 
                 Environmental sample 
                 3969 
                 2620 
                 92.8 
                 globlastp 
               
               
                 2156 
                 ZLFS24 
                 Environmental sample 
                 3970 
                 2620 
                 92.7 
                 globlastp 
               
               
                 2157 
                 ZLFS24 
                 Environmental sample 
                 3971 
                 2620 
                 88.7 
                 globlastp 
               
               
                 2158 
                 ZLFS24 
                   Pantoea  sp. 
                 3972 
                 2620 
                 88.6 
                 globlastp 
               
               
                 2159 
                 ZLFS24 
                   Pantoea  sp. 
                 3973 
                 2620 
                 87.7 
                 globlastp 
               
               
                 2160 
                 ZLFS24 
                 Environmental sample 
                 3974 
                 2620 
                 80.3 
                 globlastp 
               
               
                 2161 
                 ZLFS24 
                   Pantoea  sp. 
                 3975 
                 2620 
                 80 
                 globlastp 
               
               
                 2162 
                 ZLFS24 
                   Pantoea  sp. 
                 3976 
                 2620 
                 79.4 
                 globlastp 
               
               
                 2084 
                 ZLFS11 
                   Collimonas  sp. 
                 3898 
                 2608 
                 81.5 
                 globlastp 
               
               
                 2085 
                 ZLFS11 
                   Collimonas  sp. 
                 3899 
                 2608 
                 79 
                 globlastp 
               
               
                 825 
                 BLFS15 
                 Multievos46 sp. 
                 2677 
                 2458 
                 93.2 
                 globlastp 
               
               
                 826 
                 BLFS15 
                 Environmental sample 
                 2678 
                 2458 
                 89.2 
                 globlastp 
               
               
                 827 
                 BLFS15 
                   Arthrobacter  sp. 
                 2679 
                 2458 
                 84.4 
                 globlastp 
               
               
                 828 
                 BLFS15 
                   Arthrobacter  sp. 
                 2680 
                 2458 
                 83.6 
                 globlastp 
               
               
                 829 
                 BLFS15 
                   Arthrobacter  sp. 
                 2681 
                 2458 
                 83.4 
                 globlastp 
               
               
                 830 
                 BLFS15 
                   Arthrobacter  sp. 
                 2682 
                 2458 
                 83.2 
                 globlastp 
               
               
                 831 
                 BLFS15 
                   Arthrobacter  sp. 
                 2683 
                 2458 
                 76.9 
                 globlastp 
               
               
                 832 
                 BLFS15 
                   Arthrobacter  sp. 
                 2684 
                 2458 
                 76.7 
                 globlastp 
               
               
                 833 
                 BLFS15 
                   Arthrobacter  sp. 
                 2685 
                 2458 
                 76 
                 globlastp 
               
               
                 1753 
                 BLFS134 
                   Pseudomonas  sp. 
                 3577 
                 2569 
                 98.9 
                 globlastp 
               
               
                 1754 
                 BLFS134 
                   Pseudomonas  sp. 
                 3578 
                 2569 
                 96.8 
                 globlastp 
               
               
                 1755 
                 BLFS134 
                   Pseudomonas  sp. 
                 3579 
                 2569 
                 95.8 
                 globlastp 
               
               
                 1756 
                 BLFS134 
                   Pseudomonas  sp. 
                 3580 
                 2569 
                 93.7 
                 globlastp 
               
               
                 1757 
                 BLFS134 
                   Pseudomonas  sp. 
                 3581 
                 2569 
                 92.6 
                 globlastp 
               
               
                 1758 
                 BLFS134 
                   Pseudomonas  sp. 
                 3582 
                 2569 
                 91.6 
                 globlastp 
               
               
                 1759 
                 BLFS134 
                   Pseudomonas  sp. 
                 3583 
                 2569 
                 90.5 
                 globlastp 
               
               
                 1760 
                 BLFS134 
                   Pseudomonas  sp. 
                 3584 
                 2569 
                 89.5 
                 globlastp 
               
               
                 1761 
                 BLFS134 
                 Environmental sample 
                 3585 
                 2569 
                 88.4 
                 globlastp 
               
               
                 1762 
                 BLFS134 
                 Environmental sample 
                 3586 
                 2569 
                 87.4 
                 globlastp 
               
               
                 1763 
                 BLFS134 
                 Environmental sample 
                 3587 
                 2569 
                 86.3 
                 globlastp 
               
               
                 1764 
                 BLFS134 
                   Pseudomonas  sp. 
                 3588 
                 2569 
                 85.3 
                 globlastp 
               
               
                 1765 
                 BLFS134 
                   Pseudomonas  sp. 
                 3589 
                 2569 
                 84.2 
                 globlastp 
               
               
                 1766 
                 BLFS134 
                   Pseudomonas  sp. 
                 3590 
                 2569 
                 83.2 
                 globlastp 
               
               
                 1767 
                 BLFS134 
                   Pseudomonas  sp. 
                 3591 
                 2569 
                 82.1 
                 globlastp 
               
               
                 1768 
                 BLFS134 
                 Environmental sample 
                 3592 
                 2569 
                 81.1 
                 globlastp 
               
               
                 1769 
                 BLFS134 
                 Environmental sample 
                 3593 
                 2569 
                 80 
                 globlastp 
               
               
                 1770 
                 BLFS134 
                   Pseudomonas  sp. 
                 3594 
                 2569 
                 79.2 
                 globlastp 
               
               
                 1771 
                 BLFS134 
                 Environmental sample 
                 3595 
                 2569 
                 78.1 
                 globlastp 
               
               
                 1772 
                 BLFS134 
                   Pseudomonas  sp. 
                 3596 
                 2569 
                 77.1 
                 globlastp 
               
               
                 1773 
                 BLFS134 
                   Pseudomonas  sp. 
                 3597 
                 2569 
                 76 
                 globlastp 
               
               
                 1774 
                 BLFS134 
                   Pseudomonas  sp. 
                 3598 
                 2569 
                 75 
                 globlastp 
               
               
                 1775 
                 BLFS134 
                   Pseudomonas  sp. 
                 3599 
                 2569 
                 74 
                 globlastp 
               
               
                 1776 
                 BLFS134 
                 Environmental sample 
                 3600 
                 2569 
                 73.7 
                 globlastp 
               
               
                 1777 
                 BLFS134 
                   Pseudomonas  sp. 
                 3601 
                 2569 
                 72.4 
                 globlastp 
               
               
                 1778 
                 BLFS134 
                 Environmental sample 
                 3602 
                 2569 
                 71.6 
                 globlastp 
               
               
                 1779 
                 BLFS134 
                   Azotobacter  sp. 
                 3603 
                 2569 
                 70.5 
                 globlastp 
               
               
                 2163 
                 ZLFS25 
                   Curtobacterium  sp. 
                 3977 
                 2621 
                 95.4 
                 globlastp 
               
               
                 2164 
                 ZLFS25 
                 Environmental sample 
                 3978 
                 2621 
                 91.3 
                 globlastp 
               
               
                 2165 
                 ZLFS25 
                   Curtobacterium  sp. 
                 3979 
                 2621 
                 90.1 
                 globlastp 
               
               
                 2166 
                 ZLFS25 
                   Curtobacterium  sp. 
                 3980 
                 2621 
                 89.9 
                 globlastp 
               
               
                 2167 
                 ZLFS25 
                 Environmental sample 
                 3981 
                 2621 
                 76.6 
                 globlastp 
               
               
                 2168 
                 ZLFS25 
                 Environmental sample 
                 3982 
                 2621 
                 74.4 
                 globlastp 
               
               
                 2169 
                 ZLFS25 
                 Environmental sample 
                 3983 
                 2621 
                 73.8 
                 globlastp 
               
               
                 834 
                 BLFS16 
                 Environmental sample 
                 2686 
                 2459 
                 94.7 
                 globlastp 
               
               
                 835 
                 BLFS16 
                   Arthrobacter  sp. 
                 2687 
                 2459 
                 78.1 
                 globlastp 
               
               
                 836 
                 BLFS16 
                   Arthrobacter  sp. 
                 2688 
                 2459 
                 78 
                 globlastp 
               
               
                 837 
                 BLFS16 
                   Arthrobacter  sp. 
                 2689 
                 2459 
                 77.7 
                 globlastp 
               
               
                 838 
                 BLFS16 
                   Arthrobacter  sp. 
                 2690 
                 2459 
                 77 
                 globlastp 
               
               
                 984 
                 BLFS43 
                   Burkholderia  sp. 
                 2832 
                 2484 
                 99.3 
                 globlastp 
               
               
                 985 
                 BLFS43 
                   Burkholderia  sp. 
                 2833 
                 2484 
                 76.8 
                 globlastp 
               
               
                 986 
                 BLFS43 
                   Burkholderia  sp. 
                 2834 
                 2484 
                 71.4 
                 globlastp 
               
               
                 987 
                 BLFS43 
                   Burkholderia  sp. 
                 2835 
                 2484 
                 70.7 
                 globlastp 
               
               
                 2127 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3941 
                 2618 
                 99.1 
                 globlastp 
               
               
                 2128 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3942 
                 2618 
                 98.2 
                 globlastp 
               
               
                 2129 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3943 
                 2618 
                 97.7 
                 globlastp 
               
               
                 2130 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3944 
                 2618 
                 96.4 
                 globlastp 
               
               
                 2131 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3945 
                 2618 
                 95.9 
                 globlastp 
               
               
                 2132 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3946 
                 2618 
                 95.5 
                 globlastp 
               
               
                 2133 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3947 
                 2618 
                 95 
                 globlastp 
               
               
                 2134 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3948 
                 2618 
                 93.7 
                 globlastp 
               
               
                 2135 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3949 
                 2618 
                 93.2 
                 globlastp 
               
               
                 2136 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3950 
                 2618 
                 91.9 
                 globlastp 
               
               
                 2137 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3951 
                 2618 
                 85.6 
                 globlastp 
               
               
                 2138 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3952 
                 2618 
                 84.7 
                 globlastp 
               
               
                 2139 
                 ZLFS22 
                   Paenibacillus  sp. 
                 3953 
                 2618 
                 83.8 
                 globlastp 
               
               
                 998 
                 BLFS47 
                   Salinibacillus  sp. 
                 2846 
                 2488 
                 90.9 
                 globlastp 
               
               
                 999 
                 BLFS47 
                   Cellulosimicrobium  sp. 
                 2847 
                 2488 
                 77.3 
                 globlastp 
               
               
                 1000 
                 BLFS47 
                   Cellulosimicrobium  sp. 
                 2848 
                 2488 
                 71.8 
                 globlastp 
               
               
                 1001 
                 BLFS47 
                 Multievos46 sp. 
                 2849 
                 2488 
                 70.2 
                 globlastp 
               
               
                 1258 
                 BLFS94 
                   Paenibacillus  sp. 
                 3093 
                 2530 
                 99 
                 globlastp 
               
               
                 1259 
                 BLFS94 
                   Paenibacillus  sp. 
                 3094 
                 2530 
                 98.1 
                 globlastp 
               
               
                 1260 
                 BLFS94 
                   Paenibacillus  sp. 
                 3095 
                 2530 
                 96.3 
                 globlastp 
               
               
                 1261 
                 BLFS94 
                   Paenibacillus  sp. 
                 3096 
                 2530 
                 95 
                 globlastp 
               
               
                 1262 
                 BLFS94 
                   Paenibacillus  sp. 
                 3097 
                 2530 
                 94 
                 globlastp 
               
               
                 1263 
                 BLFS94 
                   Paenibacillus  sp. 
                 3098 
                 2530 
                 93.1 
                 globlastp 
               
               
                 1264 
                 BLFS94 
                   Paenibacillus  sp. 
                 3099 
                 2530 
                 74.6 
                 globlastp 
               
               
                 1265 
                 BLFS94 
                   Paenibacillus  sp. 
                 3100 
                 2530 
                 73.4 
                 globlastp 
               
               
                 1266 
                 BLFS94 
                   Paenibacillus  sp. 
                 3101 
                 2530 
                 71.7 
                 globlastp 
               
               
                 1104 
                 BLFS75 
                   Paenibacillus  sp. 
                 2944 
                 2513 
                 99.4 
                 globlastp 
               
               
                 1105 
                 BLFS75 
                   Paenibacillus  sp. 
                 2945 
                 2513 
                 98.8 
                 globlastp 
               
               
                 1106 
                 BLFS75 
                   Paenibacillus  sp. 
                 2946 
                 2513 
                 98.6 
                 globlastp 
               
               
                 1107 
                 BLFS75 
                   Paenibacillus  sp. 
                 2947 
                 2513 
                 98.3 
                 globlastp 
               
               
                 1108 
                 BLFS75 
                   Paenibacillus  sp. 
                 2948 
                 2513 
                 98 
                 globlastp 
               
               
                 1109 
                 BLFS75 
                   Paenibacillus  sp. 
                 2949 
                 2513 
                 97.7 
                 globlastp 
               
               
                 1110 
                 BLFS75 
                   Paenibacillus  sp. 
                 2950 
                 2513 
                 97.1 
                 globlastp 
               
               
                 1111 
                 BLFS75 
                   Paenibacillus  sp. 
                 2951 
                 2513 
                 92.5 
                 globlastp 
               
               
                 1112 
                 BLFS75 
                   Paenibacillus  sp. 
                 2952 
                 2513 
                 92.2 
                 globlastp 
               
               
                 1113 
                 BLFS75 
                   Paenibacillus  sp. 
                 2953 
                 2513 
                 91.9 
                 globlastp 
               
               
                 1114 
                 BLFS75 
                   Paenibacillus  sp. 
                 2954 
                 2513 
                 88.7 
                 globlastp 
               
               
                 1725 
                 BLFS132 
                   Pseudomonas  sp. 
                 3549 
                 2567 
                 98.5 
                 globlastp 
               
               
                 1726 
                 BLFS132 
                   Pseudomonas  sp. 
                 3550 
                 2567 
                 97.1 
                 globlastp 
               
               
                 1727 
                 BLFS132 
                   Pseudomonas  sp. 
                 3551 
                 2567 
                 95.6 
                 globlastp 
               
               
                 1728 
                 BLFS132 
                   Pseudomonas  sp. 
                 3552 
                 2567 
                 94.1 
                 globlastp 
               
               
                 1729 
                 BLFS132 
                   Pseudomonas  sp. 
                 3553 
                 2567 
                 92.6 
                 globlastp 
               
               
                 1730 
                 BLFS132 
                   Pseudomonas  sp. 
                 3554 
                 2567 
                 91.4 
                 globlastp 
               
               
                 1731 
                 BLFS132 
                   Pseudomonas  sp. 
                 3555 
                 2567 
                 88.6 
                 globlastp 
               
               
                 1732 
                 BLFS132 
                   Pseudomonas  sp. 
                 3556 
                 2567 
                 87 
                 globlastp 
               
               
                 1733 
                 BLFS132 
                   Pseudomonas  sp. 
                 3557 
                 2567 
                 85.5 
                 globlastp 
               
               
                 1734 
                 BLFS132 
                   Pseudomonas  sp. 
                 3558 
                 2567 
                 84.1 
                 globlastp 
               
               
                 1735 
                 BLFS132 
                 Environmental sample 
                 3559 
                 2567 
                 83.1 
                 globlastp 
               
               
                 1736 
                 BLFS132 
                   Pseudomonas  sp. 
                 3560 
                 2567 
                 82.6 
                 globlastp 
               
               
                 1737 
                 BLFS132 
                   Pseudomonas  sp. 
                 3561 
                 2567 
                 81.2 
                 globlastp 
               
               
                 1738 
                 BLFS132 
                   Pseudomonas  sp. 
                 3562 
                 2567 
                 80 
                 globlastp 
               
               
                 1739 
                 BLFS132 
                 Environmental sample 
                 3563 
                 2567 
                 79.4 
                 globlastp 
               
               
                 1740 
                 BLFS132 
                   Pseudomonas  sp. 
                 3564 
                 2567 
                 78.3 
                 globlastp 
               
               
                 1741 
                 BLFS132 
                   Pseudomonas  sp. 
                 3565 
                 2567 
                 77.9 
                 globlastp 
               
               
                 1742 
                 BLFS132 
                   Pseudomonas  sp. 
                 3566 
                 2567 
                 76.5 
                 globlastp 
               
               
                 1743 
                 BLFS132 
                   Pseudomonas  sp. 
                 3567 
                 2567 
                 75 
                 globlastp 
               
               
                 1744 
                 BLFS132 
                   Pseudomonas  sp. 
                 3568 
                 2567 
                 74 
                 globlastp 
               
               
                 1745 
                 BLFS132 
                   Pseudomonas  sp. 
                 3569 
                 2567 
                 73 
                 globlastp 
               
               
                 1746 
                 BLFS132 
                   Pseudomonas  sp. 
                 3570 
                 2567 
                 72 
                 globlastp 
               
               
                 1747 
                 BLFS132 
                   Pseudomonas  sp. 
                 3571 
                 2567 
                 71 
                 globlastp 
               
               
                 1748 
                 BLFS132 
                   Pseudomonas  sp. 
                 3572 
                 2567 
                 70.7 
                 globlastp 
               
               
                 809 
                 BLFS10 
                   Amycolatopsis  sp. 
                 2661 
                 2453 
                 97.4 
                 globlastp 
               
               
                 810 
                 BLFS10 
                   Amycolatopsis  sp. 
                 2662 
                 2453 
                 94.9 
                 globlastp 
               
               
                 804 
                 BLFS10 
                   Amycolatopsis  sp. 
                 2656 
                 2453 
                 94 
                 globlastp 
               
               
                 600 
                 BLFS10 
                   Amycolatopsis  sp. 
                 2452 
                 2453 
                 75.2 
                 globlastp 
               
               
                 803 
                 BLFS10 
                   Amycolatopsis  sp. 
                 2655 
                 2453 
                 74.4 
                 globlastp 
               
               
                 811 
                 BLFS10 
                   Streptomyces  sp. 
                 2663 
                 2453 
                 70.9 
                 globlastp 
               
               
                 2312 
                 ZLFS37 
                   Serratia  sp. 
                 4126 
                 2632 
                 99.1 
                 globlastp 
               
               
                 2313 
                 ZLFS37 
                 Environmental sample 
                 4127 
                 2632 
                 97.3 
                 globlastp 
               
               
                 2314 
                 ZLFS37 
                   Serratia  sp. 
                 4128 
                 2632 
                 96.4 
                 globlastp 
               
               
                 2315 
                 ZLFS37 
                   Serratia  sp. 
                 4129 
                 2632 
                 95.5 
                 globlastp 
               
               
                 2316 
                 ZLFS37 
                   Serratia  sp. 
                 4130 
                 2632 
                 94.6 
                 globlastp 
               
               
                 2317 
                 ZLFS37 
                   Serratia  sp. 
                 4131 
                 2632 
                 93.8 
                 globlastp 
               
               
                 2318 
                 ZLFS37 
                   Serratia  sp. 
                 4132 
                 2632 
                 92.9 
                 globlastp 
               
               
                 2319 
                 ZLFS37 
                   Serratia  sp. 
                 4133 
                 2632 
                 91.1 
                 globlastp 
               
               
                 2320 
                 ZLFS37 
                   Serratia  sp. 
                 4134 
                 2632 
                 84.8 
                 globlastp 
               
               
                 2321 
                 ZLFS37 
                   Serratia  sp. 
                 4135 
                 2632 
                 83.9 
                 globlastp 
               
               
                 2322 
                 ZLFS37 
                   Serratia  sp. 
                 4136 
                 2632 
                 83 
                 globlastp 
               
               
                 2323 
                 ZLFS37 
                   Yersinia  sp. 
                 4137 
                 2632 
                 82.1 
                 globlastp 
               
               
                 2324 
                 ZLFS37 
                   Serratia  sp. 
                 4138 
                 2632 
                 81.6 
                 globlastp 
               
               
                 2325 
                 ZLFS37 
                   Yersinia  sp. 
                 4139 
                 2632 
                 81.2 
                 globlastp 
               
               
                 2326 
                 ZLFS37 
                   Hafnia  sp. 
                 4140 
                 2632 
                 80.5 
                 globlastp 
               
               
                 2327 
                 ZLFS37 
                   Yersinia  sp. 
                 4141 
                 2632 
                 80.4 
                 globlastp 
               
               
                 2328 
                 ZLFS37 
                   Yersinia  sp. 
                 4142 
                 2632 
                 79.5 
                 globlastp 
               
               
                 2329 
                 ZLFS37 
                   Yersinia  sp. 
                 4143 
                 2632 
                 78.6 
                 globlastp 
               
               
                 2330 
                 ZLFS37 
                   Enterobacteriaceae  sp. 
                 4144 
                 2632 
                 77.9 
                 globlastp 
               
               
                 2331 
                 ZLFS37 
                   Rahnella  sp. 
                 4145 
                 2632 
                 77.7 
                 globlastp 
               
               
                 2332 
                 ZLFS37 
                   Ewinyella  sp. 
                 4146 
                 2632 
                 76.8 
                 globlastp 
               
               
                 2333 
                 ZLFS37 
                   Rahnella  sp. 
                 4147 
                 2632 
                 76.5 
                 globlastp 
               
               
                 2334 
                 ZLFS37 
                   Rahnella  sp. 
                 4148 
                 2632 
                 75.7 
                 globlastp 
               
               
                 2335 
                 ZLFS37 
                   Yersinia  sp. 
                 4149 
                 2632 
                 74 
                 globlastp 
               
               
                 2336 
                 ZLFS37 
                   Candidatus  sp. 
                 4150 
                 2632 
                 71.3 
                 globlastp 
               
               
                 2337 
                 ZLFS37 
                   Serratia  sp. 
                 — 
                 2632 
                 70.54 
                 glotblastn 
               
               
                 2338 
                 ZLFS37 
                   Serratia  sp. 
                 4151 
                 2632 
                 70.5 
                 globlastp 
               
               
                 2339 
                 ZLFS37 
                   Candidatus  sp. 
                 4152 
                 2632 
                 70.4 
                 globlastp 
               
               
                 2340 
                 ZLFS37 
                 gamma sp. 
                 4153 
                 2632 
                 70.2 
                 globlastp 
               
               
                 1038 
                 BLFS62 
                   Erwinia  sp. 
                 2881 
                 2502 
                 83.2 
                 globlastp 
               
               
                 1039 
                 BLFS62 
                   Pantoea  sp. 
                 2882 
                 2502 
                 81.5 
                 globlastp 
               
               
                 1040 
                 BLFS62 
                 Environmental sample 
                 2883 
                 2502 
                 79.2 
                 globlastp 
               
               
                 1041 
                 BLFS62 
                 Environmental sample 
                 2884 
                 2502 
                 78.2 
                 globlastp 
               
               
                 1042 
                 BLFS62 
                 Environmental sample 
                 2885 
                 2502 
                 77 
                 globlastp 
               
               
                 1043 
                 BLFS62 
                 Environmental sample 
                 2886 
                 2502 
                 76.2 
                 globlastp 
               
               
                 1044 
                 BLFS62 
                   Pantoea  sp. 
                 2887 
                 2502 
                 75.2 
                 globlastp 
               
               
                 1045 
                 BLFS62 
                   Pantoea  sp. 
                 2888 
                 2502 
                 74.1 
                 globlastp 
               
               
                 1046 
                 BLFS62 
                   Pantoea  sp. 
                 2889 
                 2502 
                 73.5 
                 globlastp 
               
               
                 1047 
                 BLFS62 
                   Pantoea  sp. 
                 2890 
                 2502 
                 71.5 
                 globlastp 
               
               
                 1048 
                 BLFS62 
                   Enterobacter  sp. 
                 — 
                 2502 
                 70.51 
                 glotblastn 
               
               
                 1006 
                 BLFS51 
                   Herbaspirillum  sp. 
                 — 
                 2492 
                 76.51 
                 glotblastn 
               
               
                 1007 
                 BLFS51 
                   Collimonas  sp. 
                 2854 
                 2492 
                 73.7 
                 globlastp 
               
               
                 1008 
                 BLFS51 
                   Collimonas  sp. 
                 — 
                 2492 
                 72.89 
                 glotblastn 
               
               
                 1009 
                 BLFS51 
                   Herbaspirillum  sp. 
                 2855 
                 2492 
                 70.3 
                 globlastp 
               
               
                 1701 
                 BLFS131 
                   Pseudomonas  sp. 
                 3525 
                 2566 
                 98.4 
                 globlastp 
               
               
                 1702 
                 BLFS131 
                   Pseudomonas  sp. 
                 3526 
                 2566 
                 94.5 
                 globlastp 
               
               
                 1703 
                 BLFS131 
                   Pseudomonas  sp. 
                 3527 
                 2566 
                 91.2 
                 globlastp 
               
               
                 1704 
                 BLFS131 
                   Pseudomonas  sp. 
                 3528 
                 2566 
                 90.1 
                 globlastp 
               
               
                 1705 
                 BLFS131 
                   Pseudomonas  sp. 
                 3529 
                 2566 
                 89 
                 globlastp 
               
               
                 1706 
                 BLFS131 
                   Pseudomonas  sp. 
                 3530 
                 2566 
                 88 
                 globlastp 
               
               
                 1707 
                 BLFS131 
                   Pseudomonas  sp. 
                 3531 
                 2566 
                 87 
                 globlastp 
               
               
                 1708 
                 BLFS131 
                   Pseudomonas  sp. 
                 3532 
                 2566 
                 86.3 
                 globlastp 
               
               
                 1709 
                 BLFS131 
                 Environmental sample 
                 3533 
                 2566 
                 85.2 
                 globlastp 
               
               
                 1710 
                 BLFS131 
                 Environmental sample 
                 3534 
                 2566 
                 84.1 
                 globlastp 
               
               
                 1711 
                 BLFS131 
                 Environmental sample 
                 3535 
                 2566 
                 83 
                 globlastp 
               
               
                 1712 
                 BLFS131 
                   Pseudomonas  sp. 
                 3536 
                 2566 
                 82.2 
                 globlastp 
               
               
                 1713 
                 BLFS131 
                   Pseudomonas  sp. 
                 3537 
                 2566 
                 81.1 
                 globlastp 
               
               
                 1714 
                 BLFS131 
                   Pseudomonas  sp. 
                 3538 
                 2566 
                 80 
                 globlastp 
               
               
                 1715 
                 BLFS131 
                 Environmental sample 
                 3539 
                 2566 
                 79.1 
                 globlastp 
               
               
                 1716 
                 BLFS131 
                   Pseudomonas  sp. 
                 3540 
                 2566 
                 78.1 
                 globlastp 
               
               
                 1717 
                 BLFS131 
                   Pseudomonas  sp. 
                 3541 
                 2566 
                 77 
                 globlastp 
               
               
                 1718 
                 BLFS131 
                   Pseudomonas  sp. 
                 3542 
                 2566 
                 76 
                 globlastp 
               
               
                 1719 
                 BLFS131 
                   Pseudomonas  sp. 
                 3543 
                 2566 
                 75.3 
                 globlastp 
               
               
                 1720 
                 BLFS131 
                 Environmental sample 
                 3544 
                 2566 
                 74.2 
                 globlastp 
               
               
                 1721 
                 BLFS131 
                   Pseudomonas  sp. 
                 3545 
                 2566 
                 73.1 
                 globlastp 
               
               
                 1722 
                 BLFS131 
                   Pseudomonas  sp. 
                 3546 
                 2566 
                 72 
                 globlastp 
               
               
                 1723 
                 BLFS131 
                   Pseudomonas  sp. 
                 3547 
                 2566 
                 71.4 
                 globlastp 
               
               
                 1724 
                 BLFS131 
                   Pseudomonas  sp. 
                 3548 
                 2566 
                 70.3 
                 globlastp 
               
               
                 997 
                 BLFS46 
                   Burkholderia  sp. 
                 2845 
                 2487 
                 84.9 
                 globlastp 
               
               
                 1072 
                 BLFS73 
                   Paenibacillus  sp. 
                 2914 
                 2511 
                 99.2 
                 globlastp 
               
               
                 1073 
                 BLFS73 
                   Paenibacillus  sp. 
                 2915 
                 2511 
                 98.4 
                 globlastp 
               
               
                 1074 
                 BLFS73 
                   Paenibacillus  sp. 
                 2916 
                 2511 
                 96.7 
                 globlastp 
               
               
                 1075 
                 BLFS73 
                   Paenibacillus  sp. 
                 2917 
                 2511 
                 95.9 
                 globlastp 
               
               
                 1076 
                 BLFS73 
                   Paenibacillus  sp. 
                 2918 
                 2511 
                 95.1 
                 globlastp 
               
               
                 1077 
                 BLFS73 
                   Paenibacillus  sp. 
                 2919 
                 2511 
                 84.6 
                 globlastp 
               
               
                 1078 
                 BLFS73 
                   Paenibacillus  sp. 
                 — 
                 2511 
                 82.93 
                 glotblastn 
               
               
                 1079 
                 BLFS73 
                   Paenibacillus  sp. 
                 2920 
                 2511 
                 82.1 
                 globlastp 
               
               
                 1080 
                 BLFS73 
                   Paenibacillus  sp. 
                 2921 
                 2511 
                 81.3 
                 globlastp 
               
               
                 1081 
                 BLFS73 
                   Paenibacillus  sp. 
                 2922 
                 2511 
                 78.9 
                 globlastp 
               
               
                 1082 
                 BLFS73 
                   Paenibacillus  sp. 
                 2923 
                 2511 
                 78 
                 globlastp 
               
               
                 1083 
                 BLFS73 
                   Paenibacillus  sp. 
                 2924 
                 2511 
                 77.2 
                 globlastp 
               
               
                 1084 
                 BLFS73 
                 Environmental sample 
                 2925 
                 2511 
                 76.4 
                 globlastp 
               
               
                 1085 
                 BLFS73 
                   Paenibacillus  sp. 
                 2926 
                 2511 
                 75 
                 globlastp 
               
               
                 1086 
                 BLFS73 
                   Paenibacillus  sp. 
                 2927 
                 2511 
                 74 
                 globlastp 
               
               
                 1087 
                 BLFS73 
                   Paenibacillus  sp. 
                 — 
                 2511 
                 73.17 
                 glotblastn 
               
               
                 1088 
                 BLFS73 
                   Paenibacillus  sp. 
                 2928 
                 2511 
                 72.4 
                 globlastp 
               
               
                 1089 
                 BLFS73 
                   Paenibacillus  sp. 
                 2929 
                 2511 
                 71.2 
                 globlastp 
               
               
                 1652 
                 BLFS127 
                   Pseudomonas  sp. 
                 3476 
                 2562 
                 98.3 
                 globlastp 
               
               
                 1653 
                 BLFS127 
                   Pseudomonas  sp. 
                 3477 
                 2562 
                 98.1 
                 globlastp 
               
               
                 1654 
                 BLFS127 
                   Pseudomonas  sp. 
                 3478 
                 2562 
                 97.7 
                 globlastp 
               
               
                 1655 
                 BLFS127 
                   Pseudomonas  sp. 
                 3479 
                 2562 
                 97.4 
                 globlastp 
               
               
                 1656 
                 BLFS127 
                   Pseudomonas  sp. 
                 3480 
                 2562 
                 96.7 
                 globlastp 
               
               
                 1657 
                 BLFS127 
                   Pseudomonas  sp. 
                 3481 
                 2562 
                 96 
                 globlastp 
               
               
                 1658 
                 BLFS127 
                   Pseudomonas  sp. 
                 3482 
                 2562 
                 95.8 
                 globlastp 
               
               
                 1659 
                 BLFS127 
                   Pseudomonas  sp. 
                 3483 
                 2562 
                 95.2 
                 globlastp 
               
               
                 1660 
                 BLFS127 
                   Pseudomonas  sp. 
                 3484 
                 2562 
                 86.2 
                 globlastp 
               
               
                 1661 
                 BLFS127 
                   Pseudomonas  sp. 
                 3485 
                 2562 
                 75.5 
                 globlastp 
               
               
                 1662 
                 BLFS127 
                   Pseudomonas  sp. 
                 3486 
                 2562 
                 75.3 
                 globlastp 
               
               
                 1663 
                 BLFS127 
                   Pseudomonas  sp. 
                 3487 
                 2562 
                 75.2 
                 globlastp 
               
               
                 1664 
                 BLFS127 
                   Pseudomonas  sp. 
                 3488 
                 2562 
                 75.1 
                 globlastp 
               
               
                 1665 
                 BLFS127 
                   Pseudomonas  sp. 
                 3489 
                 2562 
                 75 
                 globlastp 
               
               
                 1666 
                 BLFS127 
                   Pseudomonas  sp. 
                 3490 
                 2562 
                 74.8 
                 globlastp 
               
               
                 1667 
                 BLFS127 
                   Pseudomonas  sp. 
                 3491 
                 2562 
                 73.3 
                 globlastp 
               
               
                 1067 
                 BLFS68 
                   Janthinobacterium  sp. 
                 2909 
                 2507 
                 98.7 
                 globlastp 
               
               
                 1068 
                 BLFS68 
                   Janthinobacterium  sp. 
                 2910 
                 2507 
                 90.3 
                 globlastp 
               
               
                 1069 
                 BLFS68 
                   Janthinobacterium  sp. 
                 2911 
                 2507 
                 89.6 
                 globlastp 
               
               
                 1070 
                 BLFS68 
                   Janthinobacterium  sp. 
                 2912 
                 2507 
                 88.5 
                 globlastp 
               
               
                 1049 
                 BLFS63 
                   Erwinia  sp. 
                 2891 
                 2503 
                 85.7 
                 globlastp 
               
               
                 1050 
                 BLFS63 
                   Erwinia  sp. 
                 2892 
                 2503 
                 83.8 
                 globlastp 
               
               
                 1051 
                 BLFS63 
                 Environmental sample 
                 2893 
                 2503 
                 82.9 
                 globlastp 
               
               
                 1052 
                 BLFS63 
                 Environmental sample 
                 2894 
                 2503 
                 81 
                 globlastp 
               
               
                 1053 
                 BLFS63 
                 Environmental sample 
                 2895 
                 2503 
                 80.3 
                 globlastp 
               
               
                 1054 
                 BLFS63 
                   Erwinia  sp. 
                 2896 
                 2503 
                 79.4 
                 globlastp 
               
               
                 1055 
                 BLFS63 
                   Erwinia  sp. 
                 2897 
                 2503 
                 78 
                 globlastp 
               
               
                 1056 
                 BLFS63 
                   Pantoea  sp. 
                 2898 
                 2503 
                 76.8 
                 globlastp 
               
               
                 1057 
                 BLFS63 
                   Meta  sp. 
                 2899 
                 2503 
                 75 
                 globlastp 
               
               
                 1058 
                 BLFS63 
                   Plautia  sp. 
                 2900 
                 2503 
                 74 
                 globlastp 
               
               
                 1059 
                 BLFS63 
                   Pantoea  sp. 
                 2901 
                 2503 
                 73.2 
                 globlastp 
               
               
                 1060 
                 BLFS63 
                 Environmental sample 
                 2902 
                 2503 
                 72 
                 globlastp 
               
               
                 1061 
                 BLFS63 
                 Environmental sample 
                 2903 
                 2503 
                 71.8 
                 globlastp 
               
               
                 1002 
                 BLFS48 
                   Collimonas  sp. 
                 2850 
                 2489 
                 98.9 
                 globlastp 
               
               
                 1003 
                 BLFS48 
                   Collimonas  sp. 
                 2851 
                 2489 
                 78.9 
                 globlastp 
               
               
                 1004 
                 BLFS48 
                   Collimonas  sp. 
                 2852 
                 2489 
                 76.5 
                 globlastp 
               
               
                 2080 
                 ZLFS7 
                   Collimonas  sp. 
                 3894 
                 2604 
                 70.4 
                 globlastp 
               
               
                 937 
                 BLFS33 
                   Bacillus  sp. 
                 2787 
                 2475 
                 99.1 
                 globlastp 
               
               
                 938 
                 BLFS33 
                   Bacillus  sp. 
                 2788 
                 2475 
                 98.1 
                 globlastp 
               
               
                 939 
                 BLFS33 
                   Bacillus  sp. 
                 2789 
                 2475 
                 96.4 
                 globlastp 
               
               
                 940 
                 BLFS33 
                   Bacillus  sp. 
                 2790 
                 2475 
                 94 
                 globlastp 
               
               
                 941 
                 BLFS33 
                   Bacillus  sp. 
                 2791 
                 2475 
                 93.1 
                 globlastp 
               
               
                 942 
                 BLFS33 
                   Bacillus  sp. 
                 2792 
                 2475 
                 92.1 
                 globlastp 
               
               
                 943 
                 BLFS33 
                   Bacillus  sp. 
                 2793 
                 2475 
                 90.5 
                 globlastp 
               
               
                 944 
                 BLFS33 
                   Bacillus  sp. 
                 2794 
                 2475 
                 80 
                 globlastp 
               
               
                 945 
                 BLFS33 
                   Bacillus  sp. 
                 2795 
                 2475 
                 78.1 
                 globlastp 
               
               
                 946 
                 BLFS33 
                   Bacillus  sp. 
                 2796 
                 2475 
                 76.7 
                 globlastp 
               
               
                 947 
                 BLFS33 
                   Bacillus  sp. 
                 2797 
                 2475 
                 76 
                 globlastp 
               
               
                 948 
                 BLFS33 
                   Bacillus  sp. 
                 2798 
                 2475 
                 75.4 
                 globlastp 
               
               
                 1431 
                 BLFS110 
                 Environmental sample 
                 3257 
                 2546 
                 83.8 
                 globlastp 
               
               
                 1227 
                 BLFS91 
                   Paenibacillus  sp. 
                 3062 
                 2527 
                 99.2 
                 globlastp 
               
               
                 1228 
                 BLFS91 
                   Paenibacillus  sp. 
                 3063 
                 2527 
                 96.8 
                 globlastp 
               
               
                 1229 
                 BLFS91 
                   Paenibacillus  sp. 
                 3064 
                 2527 
                 96 
                 globlastp 
               
               
                 1230 
                 BLFS91 
                   Paenibacillus  sp. 
                 3065 
                 2527 
                 95.2 
                 globlastp 
               
               
                 1231 
                 BLFS91 
                   Paenibacillus  sp. 
                 3066 
                 2527 
                 93.6 
                 globlastp 
               
               
                 1232 
                 BLFS91 
                   Paenibacillus  sp. 
                 3067 
                 2527 
                 88.8 
                 globlastp 
               
               
                 1233 
                 BLFS91 
                   Paenibacillus  sp. 
                 3068 
                 2527 
                 87.2 
                 globlastp 
               
               
                 1234 
                 BLFS91 
                   Paenibacillus  sp. 
                 3069 
                 2527 
                 86.4 
                 globlastp 
               
               
                 1235 
                 BLFS91 
                   Paenibacillus  sp. 
                 3070 
                 2527 
                 84.8 
                 globlastp 
               
               
                 1236 
                 BLFS91 
                   Paenibacillus  sp. 
                 3071 
                 2527 
                 81.6 
                 globlastp 
               
               
                 1237 
                 BLFS91 
                   Paenibacillus  sp. 
                 3072 
                 2527 
                 80.8 
                 globlastp 
               
               
                 2255 
                 ZLFS31 
                   Serratia  sp. 
                 4069 
                 2626 
                 99.2 
                 globlastp 
               
               
                 2256 
                 ZLFS31 
                   Serratia  sp. 
                 4070 
                 2626 
                 98.8 
                 globlastp 
               
               
                 2257 
                 ZLFS31 
                   Serratia  sp. 
                 4071 
                 2626 
                 97 
                 globlastp 
               
               
                 2258 
                 ZLFS31 
                 Multievos46 sp. 
                 4072 
                 2626 
                 92.1 
                 globlastp 
               
               
                 2259 
                 ZLFS31 
                   Serratia  sp. 
                 4073 
                 2626 
                 91.3 
                 globlastp 
               
               
                 2260 
                 ZLFS31 
                   Serratia  sp. 
                 4074 
                 2626 
                 89 
                 globlastp 
               
               
                 2261 
                 ZLFS31 
                   Serratia  sp. 
                 4075 
                 2626 
                 88.2 
                 globlastp 
               
               
                 2262 
                 ZLFS31 
                   Serratia  sp. 
                 4076 
                 2626 
                 83.1 
                 globlastp 
               
               
                 2263 
                 ZLFS31 
                   Serratia  sp. 
                 4077 
                 2626 
                 82.1 
                 globlastp 
               
               
                 2264 
                 ZLFS31 
                   Serratia  sp. 
                 4078 
                 2626 
                 81 
                 globlastp 
               
               
                 2265 
                 ZLFS31 
                   Yersinia  sp. 
                 4079 
                 2626 
                 75.3 
                 globlastp 
               
               
                 2266 
                 ZLFS31 
                   Yersinia  sp. 
                 4080 
                 2626 
                 74.8 
                 globlastp 
               
               
                 2267 
                 ZLFS31 
                   Yersinia  sp. 
                 4081 
                 2626 
                 73 
                 globlastp 
               
               
                 903 
                 BLFS27 
                   Bacillus  sp. 
                 2753 
                 2470 
                 89.4 
                 globlastp 
               
               
                 904 
                 BLFS27 
                   Bacillus  sp. 
                 2754 
                 2470 
                 87.6 
                 globlastp 
               
               
                 905 
                 BLFS27 
                   Bacillus  sp. 
                 2755 
                 2470 
                 86.6 
                 globlastp 
               
               
                 906 
                 BLFS27 
                   Bacillus  sp. 
                 2756 
                 2470 
                 78.6 
                 globlastp 
               
               
                 907 
                 BLFS27 
                   Bacillus  sp. 
                 2757 
                 2470 
                 75.1 
                 globlastp 
               
               
                 1475 
                 BLFS114 
                   Pseudomonas  sp. 
                 3301 
                 2550 
                 93.6 
                 globlastp 
               
               
                 1476 
                 BLFS114 
                   Pseudomonas  sp. 
                 3302 
                 2550 
                 93.1 
                 globlastp 
               
               
                 1477 
                 BLFS114 
                   Pseudomonas  sp. 
                 3303 
                 2550 
                 92.5 
                 globlastp 
               
               
                 1478 
                 BLFS114 
                   Pseudomonas  sp. 
                 3304 
                 2550 
                 91 
                 globlastp 
               
               
                 1479 
                 BLFS114 
                 Environmental sample 
                 3305 
                 2550 
                 90.2 
                 globlastp 
               
               
                 1480 
                 BLFS114 
                 Environmental sample 
                 3306 
                 2550 
                 89 
                 globlastp 
               
               
                 1481 
                 BLFS114 
                 Environmental sample 
                 3307 
                 2550 
                 88.4 
                 globlastp 
               
               
                 1482 
                 BLFS114 
                   Pseudomonas  sp. 
                 3308 
                 2550 
                 87.3 
                 globlastp 
               
               
                 1483 
                 BLFS114 
                   Pseudomonas  sp. 
                 3309 
                 2550 
                 86.2 
                 globlastp 
               
               
                 1484 
                 BLFS114 
                   Pseudomonas  sp. 
                 3310 
                 2550 
                 85 
                 globlastp 
               
               
                 1485 
                 BLFS114 
                   Pseudomonas  sp. 
                 3311 
                 2550 
                 84.4 
                 globlastp 
               
               
                 1486 
                 BLFS114 
                   Erwinia  sp. 
                 3312 
                 2550 
                 83.2 
                 globlastp 
               
               
                 1487 
                 BLFS114 
                   Pseudomonas  sp. 
                 3313 
                 2550 
                 82.1 
                 globlastp 
               
               
                 1488 
                 BLFS114 
                   Pseudomonas  sp. 
                 3314 
                 2550 
                 81 
                 globlastp 
               
               
                 1489 
                 BLFS114 
                   Pseudomonas  sp. 
                 3315 
                 2550 
                 80.3 
                 globlastp 
               
               
                 1490 
                 BLFS114 
                 Environmental sample 
                 3316 
                 2550 
                 79.2 
                 globlastp 
               
               
                 1491 
                 BLFS114 
                 Environmental sample 
                 3317 
                 2550 
                 78 
                 globlastp 
               
               
                 1492 
                 BLFS114 
                   Pseudomonas  sp. 
                 3318 
                 2550 
                 77.3 
                 globlastp 
               
               
                 1493 
                 BLFS114 
                   Pseudomonas  sp. 
                 3319 
                 2550 
                 76 
                 globlastp 
               
               
                 1494 
                 BLFS114 
                 Environmental sample 
                 3320 
                 2550 
                 75.1 
                 globlastp 
               
               
                 1495 
                 BLFS114 
                 Environmental sample 
                 3321 
                 2550 
                 74 
                 globlastp 
               
               
                 1496 
                 BLFS114 
                 Environmental sample 
                 3322 
                 2550 
                 73.4 
                 globlastp 
               
               
                 1497 
                 BLFS114 
                   Pseudomonas  sp. 
                 3323 
                 2550 
                 72 
                 globlastp 
               
               
                 1498 
                 BLFS114 
                 Environmental sample 
                 3324 
                 2550 
                 71.1 
                 globlastp 
               
               
                 1499 
                 BLFS114 
                 Environmental sample 
                 3325 
                 2550 
                 70.5 
                 globlastp 
               
               
                 1030 
                 BLFS61 
                   Pseudomonas  sp. 
                 2873 
                 2501 
                 99 
                 globlastp 
               
               
                 1031 
                 BLFS61 
                   Pseudomonas  sp. 
                 2874 
                 2501 
                 96 
                 globlastp 
               
               
                 1032 
                 BLFS61 
                   Pseudomonas  sp. 
                 2875 
                 2501 
                 95.7 
                 globlastp 
               
               
                 1033 
                 BLFS61 
                 Environmental sample 
                 2876 
                 2501 
                 92.5 
                 globlastp 
               
               
                 1034 
                 BLFS61 
                   Pseudomonas  sp. 
                 2877 
                 2501 
                 85.5 
                 globlastp 
               
               
                 1035 
                 BLFS61 
                 Environmental sample 
                 2878 
                 2501 
                 79.2 
                 globlastp 
               
               
                 1036 
                 BLFS61 
                   Pseudomonas  sp. 
                 2879 
                 2501 
                 78.2 
                 globlastp 
               
               
                 1037 
                 BLFS61 
                   Pseudomonas  sp. 
                 2880 
                 2501 
                 74.5 
                 globlastp 
               
               
                 1577 
                 BLFS121 
                   Pseudomonas  sp. 
                 3402 
                 2556 
                 99 
                 globlastp 
               
               
                 1578 
                 BLFS121 
                   Pseudomonas  sp. 
                 3403 
                 2556 
                 98.8 
                 globlastp 
               
               
                 1579 
                 BLFS121 
                   Pseudomonas  sp. 
                 3404 
                 2556 
                 98.3 
                 globlastp 
               
               
                 1580 
                 BLFS121 
                   Pseudomonas  sp. 
                 3405 
                 2556 
                 97.8 
                 globlastp 
               
               
                 1581 
                 BLFS121 
                   Pseudomonas  sp. 
                 3406 
                 2556 
                 93.2 
                 globlastp 
               
               
                 1582 
                 BLFS121 
                   Pseudomonas  sp. 
                 3407 
                 2556 
                 92.3 
                 globlastp 
               
               
                 1583 
                 BLFS121 
                   Pseudomonas  sp. 
                 3408 
                 2556 
                 87 
                 globlastp 
               
               
                 1584 
                 BLFS121 
                   Pseudomonas  sp. 
                 3409 
                 2556 
                 86 
                 globlastp 
               
               
                 1585 
                 BLFS121 
                   Pseudomonas  sp. 
                 3410 
                 2556 
                 85 
                 globlastp 
               
               
                 1586 
                 BLFS121 
                 Environmental sample 
                 3411 
                 2556 
                 84.1 
                 globlastp 
               
               
                 1587 
                 BLFS121 
                   Pseudomonas  sp. 
                 3412 
                 2556 
                 83.1 
                 globlastp 
               
               
                 1588 
                 BLFS121 
                 Environmental sample 
                 3413 
                 2556 
                 82.1 
                 globlastp 
               
               
                 1589 
                 BLFS121 
                   Pseudomonas  sp. 
                 3414 
                 2556 
                 81.4 
                 globlastp 
               
               
                 1590 
                 BLFS121 
                   Pseudomonas  sp. 
                 3415 
                 2556 
                 80 
                 globlastp 
               
               
                 1591 
                 BLFS121 
                   Pseudomonas  sp. 
                 3416 
                 2556 
                 79.2 
                 globlastp 
               
               
                 1592 
                 BLFS121 
                 Environmental sample 
                 3417 
                 2556 
                 78 
                 globlastp 
               
               
                 1593 
                 BLFS121 
                   Pseudomonas  sp. 
                 3418 
                 2556 
                 77.5 
                 globlastp 
               
               
                 1594 
                 BLFS121 
                   Pseudomonas  sp. 
                 3419 
                 2556 
                 76.1 
                 globlastp 
               
               
                 1595 
                 BLFS121 
                   Polaromonas  sp. 
                 3420 
                 2556 
                 75.7 
                 globlastp 
               
               
                 1596 
                 BLFS121 
                   Pseudomonas  sp. 
                 3421 
                 2556 
                 74.2 
                 globlastp 
               
               
                 1597 
                 BLFS121 
                   Pseudomonas  sp. 
                 3422 
                 2556 
                 72 
                 globlastp 
               
               
                 1598 
                 BLFS121 
                   Pseudomonas  sp. 
                 3423 
                 2556 
                 71 
                 globlastp 
               
               
                 2092 
                 ZLFS15 
                   Collimonas  sp. 
                 3906 
                 2612 
                 89.2 
                 globlastp 
               
               
                 2093 
                 ZLFS15 
                   Collimonas  sp. 
                 3907 
                 2612 
                 87.2 
                 globlastp 
               
               
                 2094 
                 ZLFS15 
                   Collimonas  sp. 
                 3908 
                 2612 
                 85.6 
                 globlastp 
               
               
                 1115 
                 BLFS76 
                   Paenibacillus  sp. 
                 2955 
                 2514 
                 95.4 
                 globlastp 
               
               
                 1116 
                 BLFS76 
                   Paenibacillus  sp. 
                 2956 
                 2514 
                 94.6 
                 globlastp 
               
               
                 1117 
                 BLFS76 
                   Paenibacillus  sp. 
                 2957 
                 2514 
                 93.4 
                 globlastp 
               
               
                 1118 
                 BLFS76 
                   Paenibacillus  sp. 
                 2958 
                 2514 
                 92.7 
                 globlastp 
               
               
                 1119 
                 BLFS76 
                   Paenibacillus  sp. 
                 2959 
                 2514 
                 90 
                 globlastp 
               
               
                 1120 
                 BLFS76 
                   Paenibacillus  sp. 
                 2960 
                 2514 
                 82.6 
                 globlastp 
               
               
                 1121 
                 BLFS76 
                   Paenibacillus  sp. 
                 2961 
                 2514 
                 72.3 
                 globlastp 
               
               
                 1122 
                 BLFS76 
                   Paenibacillus  sp. 
                 — 
                 2514 
                 71.04 
                 glotblastn 
               
               
                 1123 
                 BLFS76 
                   Paenibacillus  sp. 
                 2962 
                 2514 
                 70.1 
                 globlastp 
               
               
                 1530 
                 BLFS116 
                   Pseudomonas  sp. 
                 3355 
                 2552 
                 95.8 
                 globlastp 
               
               
                 1531 
                 BLFS116 
                   Pseudomonas  sp. 
                 3356 
                 2552 
                 90.2 
                 globlastp 
               
               
                 1532 
                 BLFS116 
                   Pseudomonas  sp. 
                 3357 
                 2552 
                 89.2 
                 globlastp 
               
               
                 1533 
                 BLFS116 
                 Environmental sample 
                 3358 
                 2552 
                 88.1 
                 globlastp 
               
               
                 1534 
                 BLFS116 
                   Pseudomonas  sp. 
                 3359 
                 2552 
                 87.8 
                 globlastp 
               
               
                 1535 
                 BLFS116 
                   Pseudomonas  sp. 
                 3360 
                 2552 
                 86.3 
                 globlastp 
               
               
                 1536 
                 BLFS116 
                   Pseudomonas  sp. 
                 3361 
                 2552 
                 85.5 
                 globlastp 
               
               
                 1537 
                 BLFS116 
                   Pseudomonas  sp. 
                 3362 
                 2552 
                 84.8 
                 globlastp 
               
               
                 1538 
                 BLFS116 
                   Pseudomonas  sp. 
                 3363 
                 2552 
                 83 
                 globlastp 
               
               
                 1539 
                 BLFS116 
                   Pseudomonas  sp. 
                 3364 
                 2552 
                 82.1 
                 globlastp 
               
               
                 1540 
                 BLFS116 
                   Pseudomonas  sp. 
                 3365 
                 2552 
                 81 
                 globlastp 
               
               
                 1541 
                 BLFS116 
                   Pseudomonas  sp. 
                 3366 
                 2552 
                 80.1 
                 globlastp 
               
               
                 1542 
                 BLFS116 
                   Pseudomonas  sp. 
                 3367 
                 2552 
                 79.1 
                 globlastp 
               
               
                 1543 
                 BLFS116 
                   Pseudomonas  sp. 
                 3368 
                 2552 
                 78 
                 globlastp 
               
               
                 1544 
                 BLFS116 
                   Pseudomonas  sp. 
                 3369 
                 2552 
                 77 
                 globlastp 
               
               
                 1545 
                 BLFS116 
                   Pseudomonas  sp. 
                 3370 
                 2552 
                 76.4 
                 globlastp 
               
               
                 1546 
                 BLFS116 
                   Pseudomonas  sp. 
                 3371 
                 2552 
                 75.1 
                 globlastp 
               
               
                 1547 
                 BLFS116 
                   Pseudomonas  sp. 
                 3372 
                 2552 
                 74 
                 globlastp 
               
               
                 1548 
                 BLFS116 
                   Pseudomonas  sp. 
                 3373 
                 2552 
                 73.2 
                 globlastp 
               
               
                 1549 
                 BLFS116 
                   Pseudomonas  sp. 
                 3374 
                 2552 
                 72.3 
                 globlastp 
               
               
                 1550 
                 BLFS116 
                   Pseudomonas  sp. 
                 3375 
                 2552 
                 71 
                 globlastp 
               
               
                 1551 
                 BLFS116 
                 Environmental sample 
                 3376 
                 2552 
                 70.2 
                 globlastp 
               
               
                 1344 
                 BLFS103 
                   Pantoea  sp. 
                 3179 
                 2539 
                 94.1 
                 globlastp 
               
               
                 1345 
                 BLFS103 
                 unknown sp. 
                 3180 
                 2539 
                 89.2 
                 globlastp 
               
               
                 1346 
                 BLFS103 
                   Pantoea  sp. 
                 3181 
                 2539 
                 86.5 
                 globlastp 
               
               
                 1347 
                 BLFS103 
                   Photorhabdus  sp. 
                 — 
                 2539 
                 74.65 
                 glotblastn 
               
               
                 1348 
                 BLFS103 
                   Yersinia  sp. 
                 — 
                 2539 
                 74.31 
                 glotblastn 
               
               
                 1349 
                 BLFS103 
                   Serratia  sp. 
                 — 
                 2539 
                 73.96 
                 glotblastn 
               
               
                 1350 
                 BLFS103 
                   Salmonella  sp. 
                 — 
                 2539 
                 73.61 
                 glotblastn 
               
               
                 1351 
                 BLFS103 
                   Salmonella  sp. 
                 — 
                 2539 
                 73.26 
                 glotblastn 
               
               
                 1352 
                 BLFS103 
                   Serratia  sp. 
                 — 
                 2539 
                 73.1 
                 glotblastn 
               
               
                 1353 
                 BLFS103 
                   Yersinia  sp. 
                 3182 
                 2539 
                 71.9 
                 globlastp 
               
               
                 1354 
                 BLFS103 
                   Yersinia  sp. 
                 — 
                 2539 
                 71.53 
                 glotblastn 
               
               
                 1355 
                 BLFS103 
                   Serratia  sp. 
                 — 
                 2539 
                 71.18 
                 glotblastn 
               
               
                 1356 
                 BLFS103 
                   Pantoea  sp. 
                 — 
                 2539 
                 70.14 
                 glotblastn 
               
               
                 1668 
                 BLFS128 
                   Pseudomonas  sp. 
                 3492 
                 2563 
                 99.3 
                 globlastp 
               
               
                 1669 
                 BLFS128 
                   Pseudomonas  sp. 
                 3493 
                 2563 
                 95.2 
                 globlastp 
               
               
                 1670 
                 BLFS128 
                 Environmental sample 
                 3494 
                 2563 
                 94.3 
                 globlastp 
               
               
                 1671 
                 BLFS128 
                   Pseudomonas  sp. 
                 3495 
                 2563 
                 93.3 
                 globlastp 
               
               
                 1672 
                 BLFS128 
                   Pseudomonas  sp. 
                 3496 
                 2563 
                 92.9 
                 globlastp 
               
               
                 1673 
                 BLFS128 
                   Pseudomonas  sp. 
                 3497 
                 2563 
                 91.7 
                 globlastp 
               
               
                 1674 
                 BLFS128 
                   Pseudomonas  sp. 
                 3498 
                 2563 
                 89.2 
                 globlastp 
               
               
                 1675 
                 BLFS128 
                   Pseudomonas  sp. 
                 3499 
                 2563 
                 80.1 
                 globlastp 
               
               
                 1676 
                 BLFS128 
                   Pseudomonas  sp. 
                 3500 
                 2563 
                 79.5 
                 globlastp 
               
               
                 1677 
                 BLFS128 
                   Pseudomonas  sp. 
                 3501 
                 2563 
                 78 
                 globlastp 
               
               
                 1678 
                 BLFS128 
                   Pseudomonas  sp. 
                 3502 
                 2563 
                 77 
                 globlastp 
               
               
                 1679 
                 BLFS128 
                   Pseudomonas  sp. 
                 3503 
                 2563 
                 76 
                 globlastp 
               
               
                 1680 
                 BLFS128 
                   Pseudomonas  sp. 
                 3504 
                 2563 
                 75.7 
                 globlastp 
               
               
                 1681 
                 BLFS128 
                   Erwinia  sp. 
                 3505 
                 2563 
                 73 
                 globlastp 
               
               
                 1682 
                 BLFS128 
                   Pseudomonas  sp. 
                 3506 
                 2563 
                 72 
                 globlastp 
               
               
                 1683 
                 BLFS128 
                   Pseudomonas  sp. 
                 3507 
                 2563 
                 71 
                 globlastp 
               
               
                 1684 
                 BLFS128 
                   Pseudomonas  sp. 
                 3508 
                 2563 
                 70 
                 globlastp 
               
               
                 1937 
                 BLFS148 
                   Serratia  sp. 
                 3760 
                 2583 
                 99.2 
                 globlastp 
               
               
                 1938 
                 BLFS148 
                   Serratia  sp. 
                 3761 
                 2583 
                 98.4 
                 globlastp 
               
               
                 1939 
                 BLFS148 
                   Serratia  sp. 
                 3762 
                 2583 
                 95.2 
                 globlastp 
               
               
                 1940 
                 BLFS148 
                   Serratia  sp. 
                 3763 
                 2583 
                 89.2 
                 globlastp 
               
               
                 1941 
                 BLFS148 
                   Serratia  sp. 
                 3764 
                 2583 
                 87.2 
                 globlastp 
               
               
                 1942 
                 BLFS148 
                   Serratia  sp. 
                 3765 
                 2583 
                 86.4 
                 globlastp 
               
               
                 1943 
                 BLFS148 
                   Serratia  sp. 
                 3766 
                 2583 
                 85.6 
                 globlastp 
               
               
                 1944 
                 BLFS148 
                   Yersinia  sp. 
                 3767 
                 2583 
                 84 
                 globlastp 
               
               
                 1945 
                 BLFS148 
                   Yersinia  sp. 
                 3768 
                 2583 
                 83.2 
                 globlastp 
               
               
                 1946 
                 BLFS148 
                   Pantoea  sp. 
                 3769 
                 2583 
                 82 
                 globlastp 
               
               
                 1947 
                 BLFS148 
                   Yersinia  sp. 
                 3770 
                 2583 
                 81.2 
                 globlastp 
               
               
                 1948 
                 BLFS148 
                   Pectobacterium  sp. 
                 3771 
                 2583 
                 80 
                 globlastp 
               
               
                 1949 
                 BLFS148 
                   Pectobacterium  sp. 
                 3772 
                 2583 
                 79.2 
                 globlastp 
               
               
                 1950 
                 BLFS148 
                   Erwinia  sp. 
                 3773 
                 2583 
                 78 
                 globlastp 
               
               
                 1951 
                 BLFS148 
                 Environmental sample 
                 3774 
                 2583 
                 77.3 
                 globlastp 
               
               
                 1952 
                 BLFS148 
                   Pantoea  sp. 
                 3775 
                 2583 
                 76 
                 globlastp 
               
               
                 1953 
                 BLFS148 
                   Pantoea  sp. 
                 3776 
                 2583 
                 75 
                 globlastp 
               
               
                 1954 
                 BLFS148 
                   Buttiauxella  sp. 
                 3777 
                 2583 
                 74.2 
                 globlastp 
               
               
                 1955 
                 BLFS148 
                   Salmonella  sp. 
                 3778 
                 2583 
                 73 
                 globlastp 
               
               
                 1956 
                 BLFS148 
                   Proteus  sp. 
                 3779 
                 2583 
                 72 
                 globlastp 
               
               
                 1957 
                 BLFS148 
                   Enterobacter.2  sp. 
                 3780 
                 2583 
                 71 
                 globlastp 
               
               
                 1958 
                 BLFS148 
                 Environmental sample 
                 3781 
                 2583 
                 70.2 
                 globlastp 
               
               
                 1959 
                 BLFS148 
                   Citrobacter  sp. 
                 — 
                 2583 
                 70 
                 glotblastn 
               
               
                 859 
                 BLFS19 
                   Bacillus  sp. 
                 2711 
                 2462 
                 96.4 
                 globlastp 
               
               
                 860 
                 BLFS19 
                   Bacillus  sp. 
                 2712 
                 2462 
                 96.1 
                 globlastp 
               
               
                 861 
                 BLFS19 
                   Bacillus  sp. 
                 2713 
                 2462 
                 95.8 
                 globlastp 
               
               
                 862 
                 BLFS19 
                   Bacillus  sp. 
                 2714 
                 2462 
                 95.5 
                 globlastp 
               
               
                 863 
                 BLFS19 
                   Bacillus  sp. 
                 2715 
                 2462 
                 94.7 
                 globlastp 
               
               
                 864 
                 BLFS19 
                   Bacillus  sp. 
                 2716 
                 2462 
                 92 
                 globlastp 
               
               
                 865 
                 BLFS19 
                   Bacillus  sp. 
                 2717 
                 2462 
                 91.7 
                 globlastp 
               
               
                 988 
                 BLFS44 
                   Burkholderia  sp. 
                 2836 
                 2485 
                 99.4 
                 globlastp 
               
               
                 2095 
                 ZLFS16 
                   Collimonas  sp. 
                 3909 
                 2613 
                 94.5 
                 globlastp 
               
               
                 2096 
                 ZLFS16 
                   Collimonas  sp. 
                 3910 
                 2613 
                 85.1 
                 globlastp 
               
               
                 2097 
                 ZLFS16 
                   Herminiimonas  sp. 
                 3911 
                 2613 
                 74 
                 globlastp 
               
               
                 2098 
                 ZLFS16 
                   Duganella  sp. 
                 3912 
                 2613 
                 73 
                 globlastp 
               
               
                 2099 
                 ZLFS16 
                   Janthinobacterium  sp. 
                 3913 
                 2613 
                 72 
                 globlastp 
               
               
                 2100 
                 ZLFS16 
                   Massilia  sp. 
                 3914 
                 2613 
                 71.2 
                 globlastp 
               
               
                 2101 
                 ZLFS16 
                 Oxalobacteraceae sp. 
                 3915 
                 2613 
                 70 
                 globlastp 
               
               
                 1893 
                 BLFS145 
                   Bacillus  sp. 
                 3716 
                 2580 
                 99.2 
                 globlastp 
               
               
                 1894 
                 BLFS145 
                   Bacillus  sp. 
                 3717 
                 2580 
                 97.9 
                 globlastp 
               
               
                 1895 
                 BLFS145 
                   Bacillus  sp. 
                 3718 
                 2580 
                 94 
                 globlastp 
               
               
                 1896 
                 BLFS145 
                   Bacillus  sp. 
                 3719 
                 2580 
                 93.2 
                 globlastp 
               
               
                 1897 
                 BLFS145 
                   Bacillus  sp. 
                 3720 
                 2580 
                 92.7 
                 globlastp 
               
               
                 1898 
                 BLFS145 
                   Bacillus  sp. 
                 3721 
                 2580 
                 92.1 
                 globlastp 
               
               
                 1899 
                 BLFS145 
                   Bacillus  sp. 
                 3722 
                 2580 
                 91.1 
                 globlastp 
               
               
                 1900 
                 BLFS145 
                   Bacillus  sp. 
                 3723 
                 2580 
                 84.3 
                 globlastp 
               
               
                 1901 
                 BLFS145 
                   Bacillus  sp. 
                 3724 
                 2580 
                 78.1 
                 globlastp 
               
               
                 1902 
                 BLFS145 
                   Bacillus  sp. 
                 3725 
                 2580 
                 77.5 
                 globlastp 
               
               
                 1903 
                 BLFS145 
                   Bacillus  sp. 
                 3726 
                 2580 
                 74.2 
                 globlastp 
               
               
                 1904 
                 BLFS145 
                   Bacillus  sp. 
                 3727 
                 2580 
                 74 
                 globlastp 
               
               
                 1905 
                 BLFS145 
                   Bacillus  sp. 
                 3728 
                 2580 
                 73.2 
                 globlastp 
               
               
                 1906 
                 BLFS145 
                   Bacillus  sp. 
                 3729 
                 2580 
                 71 
                 globlastp 
               
               
                 1500 
                 BLFS115 
                   Pseudomonas  sp. 
                 3326 
                 2551 
                 98.5 
                 globlastp 
               
               
                 1501 
                 BLFS115 
                   Pseudomonas  sp. 
                 3327 
                 2551 
                 98.3 
                 globlastp 
               
               
                 1502 
                 BLFS115 
                   Pseudomonas  sp. 
                 3328 
                 2551 
                 98.1 
                 globlastp 
               
               
                 1503 
                 BLFS115 
                   Pseudomonas  sp. 
                 3329 
                 2551 
                 97.7 
                 globlastp 
               
               
                 1504 
                 BLFS115 
                   Pseudomonas  sp. 
                 3330 
                 2551 
                 95.2 
                 globlastp 
               
               
                 1505 
                 BLFS115 
                   Pseudomonas  sp. 
                 3331 
                 2551 
                 94.1 
                 globlastp 
               
               
                 1506 
                 BLFS115 
                   Pseudomonas  sp. 
                 3332 
                 2551 
                 93.1 
                 globlastp 
               
               
                 1507 
                 BLFS115 
                   Pseudomonas  sp. 
                 3333 
                 2551 
                 92 
                 globlastp 
               
               
                 1508 
                 BLFS115 
                 Environmental sample 
                 3334 
                 2551 
                 91 
                 globlastp 
               
               
                 1509 
                 BLFS115 
                   Pseudomonas  sp. 
                 3335 
                 2551 
                 90 
                 globlastp 
               
               
                 1510 
                 BLFS115 
                   Pseudomonas  sp. 
                 3336 
                 2551 
                 89.7 
                 globlastp 
               
               
                 1511 
                 BLFS115 
                   Pseudomonas  sp. 
                 3337 
                 2551 
                 88.1 
                 globlastp 
               
               
                 1512 
                 BLFS115 
                   Pseudomonas  sp. 
                 3338 
                 2551 
                 87.9 
                 globlastp 
               
               
                 1513 
                 BLFS115 
                   Pseudomonas  sp. 
                 3339 
                 2551 
                 86.2 
                 globlastp 
               
               
                 1514 
                 BLFS115 
                   Pseudomonas  sp. 
                 3340 
                 2551 
                 85.2 
                 globlastp 
               
               
                 1515 
                 BLFS115 
                   Pseudomonas  sp. 
                 3341 
                 2551 
                 85.1 
                 globlastp 
               
               
                 1516 
                 BLFS115 
                   Pseudomonas  sp. 
                 3342 
                 2551 
                 84 
                 globlastp 
               
               
                 1517 
                 BLFS115 
                   Pseudomonas  sp. 
                 3343 
                 2551 
                 83.4 
                 globlastp 
               
               
                 1518 
                 BLFS115 
                   Pseudomonas  sp. 
                 3344 
                 2551 
                 82.8 
                 globlastp 
               
               
                 1519 
                 BLFS115 
                 Environmental sample 
                 3345 
                 2551 
                 80 
                 globlastp 
               
               
                 1520 
                 BLFS115 
                 Environmental sample 
                 3346 
                 2551 
                 79 
                 globlastp 
               
               
                 1521 
                 BLFS115 
                   Pseudomonas  sp. 
                 3347 
                 2551 
                 78 
                 globlastp 
               
               
                 1522 
                 BLFS115 
                   Pseudomonas  sp. 
                 3348 
                 2551 
                 77 
                 globlastp 
               
               
                 1523 
                 BLFS115 
                   Pseudomonas  sp. 
                 3349 
                 2551 
                 76 
                 globlastp 
               
               
                 1524 
                 BLFS115 
                   Pseudomonas  sp. 
                 3350 
                 2551 
                 75.2 
                 globlastp 
               
               
                 1525 
                 BLFS115 
                 Environmental sample 
                 3351 
                 2551 
                 74.2 
                 globlastp 
               
               
                 1526 
                 BLFS115 
                 Environmental sample 
                 3352 
                 2551 
                 73 
                 globlastp 
               
               
                 1527 
                 BLFS115 
                   Azotobacter  sp. 
                 3353 
                 2551 
                 72.1 
                 globlastp 
               
               
                 1528 
                 BLFS115 
                   Pseudomonas  sp. 
                 3354 
                 2551 
                 71 
                 globlastp 
               
               
                 1529 
                 BLFS115 
                   Pseudomonas  sp. 
                 — 
                 2551 
                 70.48 
                 glotblastn 
               
               
                 1780 
                 BLFS135 
                   Erwinia  sp. 
                 3604 
                 2570 
                 97.6 
                 globlastp 
               
               
                 1781 
                 BLFS135 
                   Pseudomonas  sp. 
                 3605 
                 2570 
                 97.2 
                 globlastp 
               
               
                 1782 
                 BLFS135 
                   Pseudomonas  sp. 
                 3606 
                 2570 
                 97 
                 globlastp 
               
               
                 1783 
                 BLFS135 
                   Pseudomonas  sp. 
                 3607 
                 2570 
                 96.6 
                 globlastp 
               
               
                 1784 
                 BLFS135 
                   Pseudomonas  sp. 
                 3608 
                 2570 
                 96.4 
                 globlastp 
               
               
                 1785 
                 BLFS135 
                   Pseudomonas  sp. 
                 3609 
                 2570 
                 95.2 
                 globlastp 
               
               
                 1786 
                 BLFS135 
                 Environmental sample 
                 3610 
                 2570 
                 94.6 
                 globlastp 
               
               
                 1787 
                 BLFS135 
                   Pseudomonas  sp. 
                 3611 
                 2570 
                 88.6 
                 globlastp 
               
               
                 1788 
                 BLFS135 
                   Pseudomonas  sp. 
                 3612 
                 2570 
                 87.3 
                 globlastp 
               
               
                 1789 
                 BLFS135 
                   Pseudomonas  sp. 
                 3613 
                 2570 
                 73 
                 globlastp 
               
               
                 1790 
                 BLFS135 
                   Pseudomonas  sp. 
                 3614 
                 2570 
                 72.8 
                 globlastp 
               
               
                 1791 
                 BLFS135 
                   Pseudomonas  sp. 
                 3615 
                 2570 
                 70.9 
                 globlastp 
               
               
                 1882 
                 BLFS144 
                   Salinibacillus  sp. 
                 3705 
                 2579 
                 99.1 
                 globlastp 
               
               
                 1883 
                 BLFS144 
                   Bacillus  sp. 
                 3706 
                 2579 
                 96.9 
                 globlastp 
               
               
                 1884 
                 BLFS144 
                   Bacillus  sp. 
                 3707 
                 2579 
                 95.2 
                 globlastp 
               
               
                 1885 
                 BLFS144 
                   Bacillus  sp. 
                 3708 
                 2579 
                 94.1 
                 globlastp 
               
               
                 1886 
                 BLFS144 
                   Bacillus  sp. 
                 3709 
                 2579 
                 88 
                 globlastp 
               
               
                 1887 
                 BLFS144 
                   Bacillus  sp. 
                 3710 
                 2579 
                 87 
                 globlastp 
               
               
                 1888 
                 BLFS144 
                   Bacillus  sp. 
                 3711 
                 2579 
                 86.7 
                 globlastp 
               
               
                 1889 
                 BLFS144 
                   Bacillus  sp. 
                 3712 
                 2579 
                 85.4 
                 globlastp 
               
               
                 1890 
                 BLFS144 
                   Bacillus  sp. 
                 3713 
                 2579 
                 85 
                 globlastp 
               
               
                 1891 
                 BLFS144 
                   Bacillus  sp. 
                 3714 
                 2579 
                 84.4 
                 globlastp 
               
               
                 1892 
                 BLFS144 
                   Bacillus  sp. 
                 3715 
                 2579 
                 84.1 
                 globlastp 
               
               
                 1063 
                 BLFS65 
                   Duganella  sp. 
                 2905 
                 2505 
                 84.5 
                 globlastp 
               
               
                 2424 
                 ZLFS49 
                   Curtobacterium  sp. 
                 4233 
                 2640 
                 93.2 
                 globlastp 
               
               
                 2425 
                 ZLFS49 
                   Pantoea  sp. 
                 4234 
                 2640 
                 85.7 
                 globlastp 
               
               
                 1811 
                 BLFS139 
                   Salinibacillus  sp. 
                 3634 
                 2574 
                 99.4 
                 globlastp 
               
               
                 1812 
                 BLFS139 
                   Salinibacillus  sp. 
                 3635 
                 2574 
                 98.8 
                 globlastp 
               
               
                 1813 
                 BLFS139 
                   Bacillus  sp. 
                 3636 
                 2574 
                 97.9 
                 globlastp 
               
               
                 1814 
                 BLFS139 
                   Bacillus  sp. 
                 3637 
                 2574 
                 93.8 
                 globlastp 
               
               
                 1815 
                 BLFS139 
                   Bacillus  sp. 
                 3638 
                 2574 
                 89.5 
                 globlastp 
               
               
                 1816 
                 BLFS139 
                   Bacillus  sp. 
                 3639 
                 2574 
                 88 
                 globlastp 
               
               
                 1817 
                 BLFS139 
                   Bacillus  sp. 
                 3640 
                 2574 
                 87.3 
                 globlastp 
               
               
                 1818 
                 BLFS139 
                   Bacillus  sp. 
                 3641 
                 2574 
                 86.4 
                 globlastp 
               
               
                 1819 
                 BLFS139 
                   Bacillus  sp. 
                 3642 
                 2574 
                 85.8 
                 globlastp 
               
               
                 1820 
                 BLFS139 
                   Bacillus  sp. 
                 3643 
                 2574 
                 83.9 
                 globlastp 
               
               
                 1821 
                 BLFS139 
                   Bacillus  sp. 
                 3644 
                 2574 
                 77.3 
                 globlastp 
               
               
                 1822 
                 BLFS139 
                   Bacillus  sp. 
                 3645 
                 2574 
                 76.3 
                 globlastp 
               
               
                 1823 
                 BLFS139 
                   Bacillus  sp. 
                 3646 
                 2574 
                 75.1 
                 globlastp 
               
               
                 823 
                 BLFS14 
                 Environmental sample 
                 2675 
                 2457 
                 95.7 
                 globlastp 
               
               
                 824 
                 BLFS14 
                   Arthrobacter  sp. 
                 2676 
                 2457 
                 84 
                 globlastp 
               
               
                 1628 
                 BLFS125 
                   Pseudomonas  sp. 
                 3452 
                 2560 
                 96.6 
                 globlastp 
               
               
                 1629 
                 BLFS125 
                   Pseudomonas  sp. 
                 3453 
                 2560 
                 87.6 
                 globlastp 
               
               
                 1630 
                 BLFS125 
                   Pseudomonas  sp. 
                 3454 
                 2560 
                 86.5 
                 globlastp 
               
               
                 1631 
                 BLFS125 
                   Pseudomonas  sp. 
                 3455 
                 2560 
                 85.4 
                 globlastp 
               
               
                 1632 
                 BLFS125 
                   Pseudomonas  sp. 
                 3456 
                 2560 
                 84.3 
                 globlastp 
               
               
                 1633 
                 BLFS125 
                 Environmental sample 
                 3457 
                 2560 
                 83.1 
                 globlastp 
               
               
                 1634 
                 BLFS125 
                   Meta  sp. 
                 3458 
                 2560 
                 82 
                 globlastp 
               
               
                 1635 
                 BLFS125 
                   Pseudomonas  sp. 
                 3459 
                 2560 
                 80.9 
                 globlastp 
               
               
                 1636 
                 BLFS125 
                   Pseudomonas  sp. 
                 3460 
                 2560 
                 79.8 
                 globlastp 
               
               
                 1637 
                 BLFS125 
                   Pseudomonas  sp. 
                 3461 
                 2560 
                 78.8 
                 globlastp 
               
               
                 1638 
                 BLFS125 
                   Pseudomonas  sp. 
                 3462 
                 2560 
                 78.7 
                 globlastp 
               
               
                 1639 
                 BLFS125 
                 Environmental sample 
                 3463 
                 2560 
                 77.5 
                 globlastp 
               
               
                 1640 
                 BLFS125 
                 Environmental sample 
                 3464 
                 2560 
                 76.4 
                 globlastp 
               
               
                 1641 
                 BLFS125 
                 Environmental sample 
                 3465 
                 2560 
                 75.3 
                 globlastp 
               
               
                 1642 
                 BLFS125 
                 Environmental sample 
                 3466 
                 2560 
                 74.2 
                 globlastp 
               
               
                 1643 
                 BLFS125 
                 Environmental sample 
                 3467 
                 2560 
                 73 
                 globlastp 
               
               
                 1181 
                 BLFS86 
                   Paenibacillus  sp. 
                 3018 
                 2522 
                 95.1 
                 globlastp 
               
               
                 1182 
                 BLFS86 
                   Paenibacillus  sp. 
                 3019 
                 2522 
                 92.7 
                 globlastp 
               
               
                 1183 
                 BLFS86 
                   Paenibacillus  sp. 
                 3020 
                 2522 
                 89.2 
                 globlastp 
               
               
                 1184 
                 BLFS86 
                   Paenibacillus  sp. 
                 3021 
                 2522 
                 89 
                 globlastp 
               
               
                 1185 
                 BLFS86 
                   Paenibacillus  sp. 
                 3022 
                 2522 
                 87.8 
                 globlastp 
               
               
                 1186 
                 BLFS86 
                   Paenibacillus  sp. 
                 3023 
                 2522 
                 84.7 
                 globlastp 
               
               
                 1336 
                 BLFS102 
                 Environmental sample 
                 3171 
                 2538 
                 98.5 
                 globlastp 
               
               
                 1337 
                 BLFS102 
                 Environmental sample 
                 3172 
                 2538 
                 97.1 
                 globlastp 
               
               
                 1338 
                 BLFS102 
                   Pantoea  sp. 
                 3173 
                 2538 
                 88.2 
                 globlastp 
               
               
                 1339 
                 BLFS102 
                 Environmental sample 
                 3174 
                 2538 
                 86.8 
                 globlastp 
               
               
                 1340 
                 BLFS102 
                   Pantoea  sp. 
                 3175 
                 2538 
                 85.3 
                 globlastp 
               
               
                 1341 
                 BLFS102 
                   Pantoea  sp. 
                 3176 
                 2538 
                 80.9 
                 globlastp 
               
               
                 1342 
                 BLFS102 
                 Environmental sample 
                 3177 
                 2538 
                 76.5 
                 globlastp 
               
               
                 1343 
                 BLFS102 
                   Pantoea  sp. 
                 3178 
                 2538 
                 72.1 
                 globlastp 
               
               
                 909 
                 BLFS31 
                   Bacillus  sp. 
                 2759 
                 2473 
                 99.2 
                 globlastp 
               
               
                 910 
                 BLFS31 
                   Bacillus  sp. 
                 2760 
                 2473 
                 98.4 
                 globlastp 
               
               
                 911 
                 BLFS31 
                   Streptococcus  sp. 
                 2761 
                 2473 
                 97.7 
                 globlastp 
               
               
                 912 
                 BLFS31 
                   Bacillus  sp. 
                 2762 
                 2473 
                 96.9 
                 globlastp 
               
               
                 913 
                 BLFS31 
                   Bacillus  sp. 
                 2763 
                 2473 
                 96.1 
                 globlastp 
               
               
                 914 
                 BLFS31 
                   Bacillus  sp. 
                 2764 
                 2473 
                 95.3 
                 globlastp 
               
               
                 915 
                 BLFS31 
                   Bacillus  sp. 
                 2765 
                 2473 
                 93 
                 globlastp 
               
               
                 916 
                 BLFS31 
                   Bacillus  sp. 
                 2766 
                 2473 
                 89.1 
                 globlastp 
               
               
                 917 
                 BLFS31 
                   Bacillus  sp. 
                 2767 
                 2473 
                 86.9 
                 globlastp 
               
               
                 918 
                 BLFS31 
                   Bacillus  sp. 
                 2768 
                 2473 
                 86.2 
                 globlastp 
               
               
                 919 
                 BLFS31 
                   Bacillus  sp. 
                 2769 
                 2473 
                 85.4 
                 globlastp 
               
               
                 1437 
                 BLFS112 
                   Pseudomonas  sp. 
                 3263 
                 2548 
                 98.8 
                 globlastp 
               
               
                 1438 
                 BLFS112 
                   Pseudomonas  sp. 
                 3264 
                 2548 
                 96.8 
                 globlastp 
               
               
                 1439 
                 BLFS112 
                   Pseudomonas  sp. 
                 3265 
                 2548 
                 94.7 
                 globlastp 
               
               
                 1440 
                 BLFS112 
                   Pseudomonas  sp. 
                 3266 
                 2548 
                 94.3 
                 globlastp 
               
               
                 1441 
                 BLFS112 
                   Pseudomonas  sp. 
                 3267 
                 2548 
                 93.1 
                 globlastp 
               
               
                 1442 
                 BLFS112 
                   Pseudomonas  sp. 
                 3268 
                 2548 
                 91.8 
                 globlastp 
               
               
                 1443 
                 BLFS112 
                   Pseudomonas  sp. 
                 3269 
                 2548 
                 91.4 
                 globlastp 
               
               
                 1444 
                 BLFS112 
                   Pseudomonas  sp. 
                 3270 
                 2548 
                 90.2 
                 globlastp 
               
               
                 1445 
                 BLFS112 
                   Pseudomonas  sp. 
                 3271 
                 2548 
                 89.4 
                 globlastp 
               
               
                 1446 
                 BLFS112 
                   Pseudomonas  sp. 
                 3272 
                 2548 
                 89 
                 globlastp 
               
               
                 1447 
                 BLFS112 
                   Pseudomonas  sp. 
                 3273 
                 2548 
                 74.8 
                 globlastp 
               
               
                 1448 
                 BLFS112 
                   Pseudomonas  sp. 
                 3274 
                 2548 
                 72.9 
                 globlastp 
               
               
                 1449 
                 BLFS112 
                   Pseudomonas  sp. 
                 3275 
                 2548 
                 71.4 
                 globlastp 
               
               
                 2268 
                 ZLFS32 
                   Serratia  sp. 
                 4082 
                 2627 
                 99.2 
                 globlastp 
               
               
                 2269 
                 ZLFS32 
                   Serratia  sp. 
                 4083 
                 2627 
                 97 
                 globlastp 
               
               
                 2270 
                 ZLFS32 
                   Serratia  sp. 
                 4084 
                 2627 
                 96.2 
                 globlastp 
               
               
                 2271 
                 ZLFS32 
                   Serratia  sp. 
                 4085 
                 2627 
                 88.1 
                 globlastp 
               
               
                 2272 
                 ZLFS32 
                   Serratia  sp. 
                 4086 
                 2627 
                 87 
                 globlastp 
               
               
                 2273 
                 ZLFS32 
                   Serratia  sp. 
                 4087 
                 2627 
                 86.1 
                 globlastp 
               
               
                 2274 
                 ZLFS32 
                   Serratia  sp. 
                 4088 
                 2627 
                 85 
                 globlastp 
               
               
                 2275 
                 ZLFS32 
                   Serratia  sp. 
                 4089 
                 2627 
                 84.6 
                 globlastp 
               
               
                 2276 
                 ZLFS32 
                   Serratia  sp. 
                 4090 
                 2627 
                 81.1 
                 globlastp 
               
               
                 2277 
                 ZLFS32 
                   Serratia  sp. 
                 4091 
                 2627 
                 80.9 
                 globlastp 
               
               
                 2278 
                 ZLFS32 
                   Serratia  sp. 
                 4092 
                 2627 
                 79.5 
                 globlastp 
               
               
                 2279 
                 ZLFS32 
                   Serratia  sp. 
                 4093 
                 2627 
                 78.6 
                 globlastp 
               
               
                 2280 
                 ZLFS32 
                   Serratia  sp. 
                 4094 
                 2627 
                 74.1 
                 globlastp 
               
               
                 2281 
                 ZLFS32 
                   Serratia  sp. 
                 4095 
                 2627 
                 73.7 
                 globlastp 
               
               
                 2282 
                 ZLFS32 
                   Yersinia  sp. 
                 4096 
                 2627 
                 72.4 
                 globlastp 
               
               
                 2283 
                 ZLFS32 
                   Pectobacterium  sp. 
                 4097 
                 2627 
                 70.1 
                 globlastp 
               
               
                 1793 
                 BLFS137 
                 Environmental sample 
                 3617 
                 2572 
                 98.2 
                 globlastp 
               
               
                 1794 
                 BLFS137 
                   Rhodococcus  sp. 
                 3618 
                 2572 
                 94.7 
                 globlastp 
               
               
                 1795 
                 BLFS137 
                   Rhodococcus  sp. 
                 3619 
                 2572 
                 94.3 
                 globlastp 
               
               
                 1796 
                 BLFS137 
                 Environmental sample 
                 3620 
                 2572 
                 81.1 
                 globlastp 
               
               
                 1797 
                 BLFS137 
                 Environmental sample 
                 3621 
                 2572 
                 80.8 
                 globlastp 
               
               
                 1292 
                 BLFS99 
                 Environmental sample 
                 3127 
                 2535 
                 98.9 
                 globlastp 
               
               
                 1293 
                 BLFS99 
                   Pantoea  sp. 
                 3128 
                 2535 
                 97.7 
                 globlastp 
               
               
                 1294 
                 BLFS99 
                 Environmental sample 
                 3129 
                 2535 
                 96.6 
                 globlastp 
               
               
                 1295 
                 BLFS99 
                   Pantoea  sp. 
                 3130 
                 2535 
                 93.2 
                 globlastp 
               
               
                 1296 
                 BLFS99 
                   Pantoea  sp. 
                 3131 
                 2535 
                 90.9 
                 globlastp 
               
               
                 1297 
                 BLFS99 
                   Pantoea  sp. 
                 3132 
                 2535 
                 81.1 
                 globlastp 
               
               
                 1298 
                 BLFS99 
                 Environmental sample 
                 3133 
                 2535 
                 80 
                 globlastp 
               
               
                 1299 
                 BLFS99 
                   Pantoea  sp. 
                 3134 
                 2535 
                 75 
                 globlastp 
               
               
                 1300 
                 BLFS99 
                 Environmental sample 
                 3135 
                 2535 
                 73.3 
                 globlastp 
               
               
                 1301 
                 BLFS99 
                   Pantoea  sp. 
                 3136 
                 2535 
                 72.2 
                 globlastp 
               
               
                 1302 
                 BLFS99 
                   Pantoea  sp. 
                 3137 
                 2535 
                 71.6 
                 globlastp 
               
               
                 1303 
                 BLFS99 
                   Pantoea  sp. 
                 3138 
                 2535 
                 70.5 
                 globlastp 
               
               
                 1027 
                 BLFS60 
                   Streptomyces  sp. 
                 2870 
                 2500 
                 75.2 
                 globlastp 
               
               
                 1028 
                 BLFS60 
                   Streptomyces  sp. 
                 2871 
                 2500 
                 72.3 
                 globlastp 
               
               
                 1029 
                 BLFS60 
                   Streptomyces  sp. 
                 2872 
                 2500 
                 71.8 
                 globlastp 
               
               
                 2102 
                 ZLFS17 
                 Environmental sample 
                 3916 
                 2614 
                 73.5 
                 globlastp 
               
               
                 971 
                 BLFS39 
                   Brevibacillus  sp. 
                 2819 
                 2481 
                 98.4 
                 globlastp 
               
               
                 972 
                 BLFS39 
                   Brevibacillus  sp. 
                 2820 
                 2481 
                 98.1 
                 globlastp 
               
               
                 973 
                 BLFS39 
                   Brevibacillus  sp. 
                 2821 
                 2481 
                 97.8 
                 globlastp 
               
               
                 2364 
                 ZLFS39 
                   Serratia  sp. 
                 4177 
                 2634 
                 98.8 
                 globlastp 
               
               
                 2365 
                 ZLFS39 
                   Serratia  sp. 
                 4178 
                 2634 
                 98.5 
                 globlastp 
               
               
                 2366 
                 ZLFS39 
                   Serratia  sp. 
                 4179 
                 2634 
                 98.3 
                 globlastp 
               
               
                 2367 
                 ZLFS39 
                   Serratia  sp. 
                 4180 
                 2634 
                 92.6 
                 globlastp 
               
               
                 2368 
                 ZLFS39 
                   Serratia  sp. 
                 4181 
                 2634 
                 91.4 
                 globlastp 
               
               
                 2369 
                 ZLFS39 
                 Multievos46 sp. 
                 4182 
                 2634 
                 91.2 
                 globlastp 
               
               
                 2370 
                 ZLFS39 
                   Serratia  sp. 
                 4183 
                 2634 
                 91.1 
                 globlastp 
               
               
                 2371 
                 ZLFS39 
                 Environmental sample 
                 4184 
                 2634 
                 90.9 
                 globlastp 
               
               
                 2372 
                 ZLFS39 
                   Serratia  sp. 
                 4185 
                 2634 
                 87.3 
                 globlastp 
               
               
                 2373 
                 ZLFS39 
                   Serratia  sp. 
                 4186 
                 2634 
                 87.1 
                 globlastp 
               
               
                 2374 
                 ZLFS39 
                   Serratia  sp. 
                 4187 
                 2634 
                 87 
                 globlastp 
               
               
                 2375 
                 ZLFS39 
                   Serratia  sp. 
                 4188 
                 2634 
                 86.8 
                 globlastp 
               
               
                 2376 
                 ZLFS39 
                   Serratia  sp. 
                 4189 
                 2634 
                 86.7 
                 globlastp 
               
               
                 2377 
                 ZLFS39 
                   Serratia  sp. 
                 4190 
                 2634 
                 86.5 
                 globlastp 
               
               
                 2378 
                 ZLFS39 
                 Environmental sample 
                 4191 
                 2634 
                 86.4 
                 globlastp 
               
               
                 2379 
                 ZLFS39 
                   Serratia  sp. 
                 4192 
                 2634 
                 86.2 
                 globlastp 
               
               
                 2380 
                 ZLFS39 
                   Serratia  sp. 
                 4193 
                 2634 
                 86.1 
                 globlastp 
               
               
                 2381 
                 ZLFS39 
                   Serratia  sp. 
                 4194 
                 2634 
                 85.8 
                 globlastp 
               
               
                 2382 
                 ZLFS39 
                   Serratia  sp. 
                 4195 
                 2634 
                 85.6 
                 globlastp 
               
               
                 2383 
                 ZLFS39 
                   Serratia  sp. 
                 4196 
                 2634 
                 85.2 
                 globlastp 
               
               
                 2384 
                 ZLFS39 
                   Serratia  sp. 
                 4197 
                 2634 
                 85 
                 globlastp 
               
               
                 2385 
                 ZLFS39 
                   Serratia  sp. 
                 4198 
                 2634 
                 84.9 
                 globlastp 
               
               
                 2386 
                 ZLFS39 
                   Serratia  sp. 
                 4199 
                 2634 
                 84.4 
                 globlastp 
               
               
                 2387 
                 ZLFS39 
                   Serratia  sp. 
                 4200 
                 2634 
                 79.6 
                 globlastp 
               
               
                 2388 
                 ZLFS39 
                   Serratia  sp. 
                 4201 
                 2634 
                 79.2 
                 globlastp 
               
               
                 2209 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4023 
                 2624 
                 95 
                 globlastp 
               
               
                 2210 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4024 
                 2624 
                 94.1 
                 globlastp 
               
               
                 2211 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4025 
                 2624 
                 93.1 
                 globlastp 
               
               
                 2212 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4026 
                 2624 
                 92.2 
                 globlastp 
               
               
                 2213 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4027 
                 2624 
                 91.6 
                 globlastp 
               
               
                 2214 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4028 
                 2624 
                 90.8 
                 globlastp 
               
               
                 2215 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4029 
                 2624 
                 89.1 
                 globlastp 
               
               
                 2216 
                 ZLFS29 
                 Environmental sample 
                 4030 
                 2624 
                 88.1 
                 globlastp 
               
               
                 2217 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4031 
                 2624 
                 87 
                 globlastp 
               
               
                 2218 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4032 
                 2624 
                 86.4 
                 globlastp 
               
               
                 2219 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4033 
                 2624 
                 85.7 
                 globlastp 
               
               
                 2220 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4034 
                 2624 
                 84.1 
                 globlastp 
               
               
                 2221 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4035 
                 2624 
                 83 
                 globlastp 
               
               
                 2222 
                 ZLFS29 
                 Environmental sample 
                 4036 
                 2624 
                 82 
                 globlastp 
               
               
                 2223 
                 ZLFS29 
                 Environmental sample 
                 4037 
                 2624 
                 81 
                 globlastp 
               
               
                 2224 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4038 
                 2624 
                 80.3 
                 globlastp 
               
               
                 2225 
                 ZLFS29 
                 Environmental sample 
                 4039 
                 2624 
                 79.1 
                 globlastp 
               
               
                 2226 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4040 
                 2624 
                 78 
                 globlastp 
               
               
                 2227 
                 ZLFS29 
                 Environmental sample 
                 4041 
                 2624 
                 77.4 
                 globlastp 
               
               
                 2228 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4042 
                 2624 
                 76.1 
                 globlastp 
               
               
                 2229 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4043 
                 2624 
                 75.3 
                 globlastp 
               
               
                 2230 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4044 
                 2624 
                 74.4 
                 globlastp 
               
               
                 2231 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4045 
                 2624 
                 73.4 
                 globlastp 
               
               
                 2232 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4046 
                 2624 
                 72.1 
                 globlastp 
               
               
                 2233 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4047 
                 2624 
                 71.1 
                 globlastp 
               
               
                 2234 
                 ZLFS29 
                   Pseudomonas  sp. 
                 4048 
                 2624 
                 70.2 
                 globlastp 
               
               
                 1986 
                 BLFS153 
                   Streptomyces  sp. 
                 3803 
                 2588 
                 98.6 
                 globlastp 
               
               
                 1987 
                 BLFS153 
                   Streptomyces  sp. 
                 3804 
                 2588 
                 93.3 
                 globlastp 
               
               
                 1988 
                 BLFS153 
                   Streptomyces  sp. 
                 3805 
                 2588 
                 71.7 
                 globlastp 
               
               
                 1989 
                 BLFS153 
                   Streptomyces  sp. 
                 3806 
                 2588 
                 70.9 
                 globlastp 
               
               
                 1990 
                 BLFS153 
                   Streptomyces  sp. 
                 — 
                 2588 
                 70.56 
                 glotblastn 
               
               
                 1991 
                 BLFS153 
                   Streptomyces  sp. 
                 3807 
                 2588 
                 70.4 
                 globlastp 
               
               
                 1992 
                 BLFS153 
                   Streptomyces  sp. 
                 — 
                 2588 
                 70.36 
                 glotblastn 
               
               
                 1993 
                 BLFS153 
                   Streptomyces  sp. 
                 3808 
                 2588 
                 70.1 
                 globlastp 
               
               
                 1994 
                 BLFS153 
                   Streptomyces  sp. 
                 — 
                 2588 
                 70 
                 glotblastn 
               
               
                 2086 
                 ZLFS12 
                   Collimonas  sp. 
                 3900 
                 2609 
                 81.4 
                 globlastp 
               
               
                 2087 
                 ZLFS12 
                   Collimonas  sp. 
                 3901 
                 2609 
                 81 
                 globlastp 
               
               
                 2088 
                 ZLFS12 
                   Collimonas  sp. 
                 3902 
                 2609 
                 73.7 
                 globlastp 
               
               
                 1574 
                 BLFS120 
                   Pseudomonas  sp. 
                 3399 
                 2555 
                 97.2 
                 globlastp 
               
               
                 1575 
                 BLFS120 
                   Pseudomonas  sp. 
                 3400 
                 2555 
                 84.8 
                 globlastp 
               
               
                 1576 
                 BLFS120 
                   Pseudomonas  sp. 
                 3401 
                 2555 
                 75.6 
                 globlastp 
               
               
                 2391 
                 ZLFS45 
                   Streptomyces  sp. 
                 4204 
                 2637 
                 98.2 
                 globlastp 
               
               
                 2392 
                 ZLFS45 
                   Streptomyces  sp. 
                 4205 
                 2637 
                 97.3 
                 globlastp 
               
               
                 2390 
                 ZLFS45 
                   Streptomyces  sp. 
                 4203 
                 2637 
                 96.4 
                 globlastp 
               
               
                 2389 
                 ZLFS45 
                   Streptomyces  sp. 
                 4202 
                 2637 
                 94 
                 globlastp 
               
               
                 2397 
                 ZLFS45 
                   Streptomyces  sp. 
                 4209 
                 2637 
                 90.2 
                 globlastp 
               
               
                 2394 
                 ZLFS45 
                   Streptomyces  sp. 
                 4207 
                 2637 
                 90.2 
                 globlastp 
               
               
                 2395 
                 ZLFS45 
                   Streptomyces  sp. 
                 4207 
                 2637 
                 90.2 
                 globlastp 
               
               
                 2393 
                 ZLFS45 
                   Streptomyces  sp. 
                 4206 
                 2637 
                 80.4 
                 globlastp 
               
               
                 2423 
                 ZLFS45 
                   Streptomyces  sp. 
                 4232 
                 2637 
                 73.2 
                 globlastp 
               
               
                 2398 
                 ZLFS45 
                   Streptomyces  sp. 
                 4210 
                 2637 
                 72.6 
                 globlastp 
               
               
                 2396 
                 ZLFS45 
                   Streptomyces  sp. 
                 4208 
                 2637 
                 72.6 
                 globlastp 
               
               
                 2399 
                 ZLFS44 
                   Streptomyces  sp. 
                 4211 
                 2636 
                 98.7 
                 globlastp 
               
               
                 2400 
                 ZLFS44 
                   Streptomyces  sp. 
                 4212 
                 2636 
                 98.1 
                 globlastp 
               
               
                 2401 
                 ZLFS44 
                   Streptomyces  sp. 
                 4213 
                 2636 
                 96.8 
                 globlastp 
               
               
                 2402 
                 ZLFS44 
                   Streptomyces  sp. 
                 4214 
                 2636 
                 75.8 
                 globlastp 
               
               
                 2403 
                 ZLFS44 
                   Streptomyces  sp. 
                 4215 
                 2636 
                 75.2 
                 globlastp 
               
               
                 2404 
                 ZLFS44 
                   Microtetraspora  sp. 
                 4216 
                 2636 
                 74.7 
                 globlastp 
               
               
                 2405 
                 ZLFS44 
                   Streptomyces  sp. 
                 4217 
                 2636 
                 74.5 
                 globlastp 
               
               
                 2406 
                 ZLFS44 
                   Streptomyces  sp. 
                 4218 
                 2636 
                 74.1 
                 globlastp 
               
               
                 2407 
                 ZLFS44 
                   Streptomyces  sp. 
                 4219 
                 2636 
                 73.9 
                 globlastp 
               
               
                 2408 
                 ZLFS44 
                   Streptomyces  sp. 
                 — 
                 2636 
                 73.89 
                 glotblastn 
               
               
                 2409 
                 ZLFS44 
                   Streptomyces  sp. 
                 4220 
                 2636 
                 73.4 
                 globlastp 
               
               
                 2410 
                 ZLFS44 
                   Streptomyces  sp. 
                 4221 
                 2636 
                 73.2 
                 globlastp 
               
               
                 2411 
                 ZLFS44 
                   Streptomyces  sp. 
                 4222 
                 2636 
                 72.8 
                 globlastp 
               
               
                 2412 
                 ZLFS44 
                   Streptomyces  sp. 
                 4223 
                 2636 
                 72.6 
                 globlastp 
               
               
                 2413 
                 ZLFS44 
                   Streptomyces  sp. 
                 4224 
                 2636 
                 72.2 
                 globlastp 
               
               
                 2414 
                 ZLFS44 
                 Environmental sample 
                 4225 
                 2636 
                 72 
                 globlastp 
               
               
                 2415 
                 ZLFS44 
                   Streptomyces  sp. 
                 — 
                 2636 
                 71.52 
                 glotblastn 
               
               
                 2416 
                 ZLFS44 
                   Streptomyces  sp. 
                 4226 
                 2636 
                 71.5 
                 globlastp 
               
               
                 2417 
                 ZLFS44 
                   Streptomyces  sp. 
                 4227 
                 2636 
                 71.3 
                 globlastp 
               
               
                 2418 
                 ZLFS44 
                   Streptomyces  sp. 
                 4228 
                 2636 
                 71.1 
                 globlastp 
               
               
                 2419 
                 ZLFS44 
                   Streptomyces  sp. 
                 4229 
                 2636 
                 70.8 
                 globlastp 
               
               
                 2420 
                 ZLFS44 
                   Streptomyces  sp. 
                 4230 
                 2636 
                 70.7 
                 globlastp 
               
               
                 2421 
                 ZLFS44 
                   Streptomyces  sp. 
                 4231 
                 2636 
                 70.1 
                 globlastp 
               
               
                 2422 
                 ZLFS44 
                   Streptomyces  sp. 
                 — 
                 2636 
                 70.06 
                 glotblastn 
               
               
                 1022 
                 BLFS57 
                   Duganella  sp. 
                 2865 
                 2497 
                 99 
                 globlastp 
               
               
                 1023 
                 BLFS57 
                   Janthinobacterium  sp. 
                 2866 
                 2497 
                 98 
                 globlastp 
               
               
                 1024 
                 BLFS57 
                 Oxalobacteraceae sp. 
                 2867 
                 2497 
                 85.3 
                 globlastp 
               
               
                 1368 
                 BLFS105 
                   Pantoea  sp. 
                 3194 
                 2541 
                 96.2 
                 globlastp 
               
               
                 1369 
                 BLFS105 
                 Environmental sample 
                 3195 
                 2541 
                 92.4 
                 globlastp 
               
               
                 1370 
                 BLFS105 
                 Environmental sample 
                 3196 
                 2541 
                 91.7 
                 globlastp 
               
               
                 1371 
                 BLFS105 
                 Environmental sample 
                 3197 
                 2541 
                 87.9 
                 globlastp 
               
               
                 1372 
                 BLFS105 
                 Environmental sample 
                 3198 
                 2541 
                 87.1 
                 globlastp 
               
               
                 1373 
                 BLFS105 
                 Environmental sample 
                 3199 
                 2541 
                 86.4 
                 globlastp 
               
               
                 1374 
                 BLFS105 
                 Environmental sample 
                 3200 
                 2541 
                 85.7 
                 globlastp 
               
               
                 1375 
                 BLFS105 
                 Environmental sample 
                 3201 
                 2541 
                 84.8 
                 globlastp 
               
               
                 1376 
                 BLFS105 
                 Environmental sample 
                 3202 
                 2541 
                 84.1 
                 globlastp 
               
               
                 1377 
                 BLFS105 
                   Pantoea  sp. 
                 3203 
                 2541 
                 82.6 
                 globlastp 
               
               
                 1378 
                 BLFS105 
                   Pantoea  sp. 
                 3204 
                 2541 
                 79.5 
                 globlastp 
               
               
                 1379 
                 BLFS105 
                   Erwinia  sp. 
                 3205 
                 2541 
                 75.8 
                 globlastp 
               
               
                 1380 
                 BLFS105 
                   Ewingella  sp. 
                 3206 
                 2541 
                 74.2 
                 globlastp 
               
               
                 1267 
                 BLFS95 
                   Paenibacillus  sp. 
                 3102 
                 2531 
                 97.4 
                 globlastp 
               
               
                 1268 
                 BLFS95 
                   Paenibacillus  sp. 
                 3103 
                 2531 
                 96.1 
                 globlastp 
               
               
                 1269 
                 BLFS95 
                   Paenibacillus  sp. 
                 3104 
                 2531 
                 90.8 
                 globlastp 
               
               
                 1270 
                 BLFS95 
                   Paenibacillus  sp. 
                 3105 
                 2531 
                 90.2 
                 globlastp 
               
               
                 1271 
                 BLFS95 
                   Paenibacillus  sp. 
                 3106 
                 2531 
                 89.5 
                 globlastp 
               
               
                 1272 
                 BLFS95 
                   Paenibacillus  sp. 
                 3107 
                 2531 
                 88.2 
                 globlastp 
               
               
                 1273 
                 BLFS95 
                   Paenibacillus  sp. 
                 3108 
                 2531 
                 78.4 
                 globlastp 
               
               
                 1274 
                 BLFS95 
                   Paenibacillus  sp. 
                 3109 
                 2531 
                 77.8 
                 globlastp 
               
               
                 1275 
                 BLFS95 
                   Paenibacillus  sp. 
                 3110 
                 2531 
                 75.2 
                 globlastp 
               
               
                 967 
                 BLFS37 
                   Brevibacillus  sp. 
                 2816 
                 2479 
                 99.1 
                 globlastp 
               
               
                 968 
                 BLFS37 
                   Brevibacillus  sp. 
                 2817 
                 2479 
                 98.2 
                 globlastp 
               
               
                 969 
                 BLFS37 
                   Brevibacillus  sp. 
                 — 
                 2479 
                 97.35 
                 glotblastn 
               
               
                 1570 
                 BLFS119 
                   Pseudomonas  sp. 
                 3395 
                 2554 
                 96.2 
                 globlastp 
               
               
                 1571 
                 BLFS119 
                   Pseudomonas  sp. 
                 3396 
                 2554 
                 95 
                 globlastp 
               
               
                 1572 
                 BLFS119 
                   Pseudomonas  sp. 
                 3397 
                 2554 
                 92.5 
                 globlastp 
               
               
                 1573 
                 BLFS119 
                   Pseudomonas  sp. 
                 3398 
                 2554 
                 91.2 
                 globlastp 
               
               
                 2284 
                 ZLFS33 
                   Serratia  sp. 
                 4098 
                 2628 
                 99.2 
                 globlastp 
               
               
                 2285 
                 ZLFS33 
                   Serratia  sp. 
                 4099 
                 2628 
                 96.4 
                 globlastp 
               
               
                 1025 
                 BLFS58 
                   Janthinobacterium  sp. 
                 2868 
                 2498 
                 97.4 
                 globlastp 
               
               
                 1026 
                 BLFS58 
                 Oxalobacteraceae sp. 
                 2869 
                 2498 
                 83.5 
                 globlastp 
               
               
                 2002 
                 PUB6 
                   Bacillus  sp. 
                 3816 
                 2595 
                 98.6 
                 globlastp 
               
               
                 2003 
                 PUB6 
                   Bacillus  sp. 
                 3817 
                 2595 
                 98.5 
                 globlastp 
               
               
                 2004 
                 PUB6 
                   Bacillus  sp. 
                 3818 
                 2595 
                 98.4 
                 globlastp 
               
               
                 2005 
                 PUB6 
                   Bacillus  sp. 
                 3819 
                 2595 
                 97.9 
                 globlastp 
               
               
                 2006 
                 PUB6 
                   Bacillus  sp. 
                 3820 
                 2595 
                 96.1 
                 globlastp 
               
               
                 2007 
                 PUB6 
                   Bacillus  sp. 
                 3821 
                 2595 
                 94.7 
                 globlastp 
               
               
                 2008 
                 PUB6 
                   Bacillus  sp. 
                 3822 
                 2595 
                 94.2 
                 globlastp 
               
               
                 2009 
                 PUB6 
                   Bacillus  sp. 
                 3823 
                 2595 
                 93.2 
                 globlastp 
               
               
                 2010 
                 PUB6 
                   Bacillus  sp. 
                 3824 
                 2595 
                 92.6 
                 globlastp 
               
               
                 2011 
                 PUB6 
                   Bacillus  sp. 
                 3825 
                 2595 
                 92.1 
                 globlastp 
               
               
                 792 
                 BLFS5 
                   Streptomyces  sp. 
                 2644 
                 2448 
                 76.4 
                 globlastp 
               
               
                 793 
                 BLFS5 
                   Streptomyces  sp. 
                 2645 
                 2448 
                 75.5 
                 globlastp 
               
               
                 794 
                 BLFS5 
                   Kitasatospora  sp. 
                 2646 
                 2448 
                 74.5 
                 globlastp 
               
               
                 795 
                 BLFS5 
                   Kitasatospora  sp. 
                 2647 
                 2448 
                 73.8 
                 globlastp 
               
               
                 796 
                 BLFS5 
                   Streptomyces  sp. 
                 2648 
                 2448 
                 73.2 
                 globlastp 
               
               
                 797 
                 BLFS5 
                   Kitasatospora  sp. 
                 2649 
                 2448 
                 72 
                 globlastp 
               
               
                 798 
                 BLFS5 
                   Streptomyces  sp. 
                 2650 
                 2448 
                 71.1 
                 globlastp 
               
               
                 799 
                 BLFS5 
                   Streptomyces  sp. 
                 2651 
                 2448 
                 70.1 
                 globlastp 
               
               
                 989 
                 BLFS45 
                   Burkholderia  sp. 
                 2837 
                 2486 
                 97.9 
                 globlastp 
               
               
                 990 
                 BLFS45 
                 Environmental sample 
                 2838 
                 2486 
                 82.3 
                 globlastp 
               
               
                 991 
                 BLFS45 
                   Burkholderia  sp. 
                 2839 
                 2486 
                 81.1 
                 globlastp 
               
               
                 992 
                 BLFS45 
                   Burkholderia  sp. 
                 2840 
                 2486 
                 80.2 
                 globlastp 
               
               
                 993 
                 BLFS45 
                   Burkholderia  sp. 
                 2841 
                 2486 
                 79.9 
                 globlastp 
               
               
                 994 
                 BLFS45 
                   Burkholderia  sp. 
                 2842 
                 2486 
                 76.1 
                 globlastp 
               
               
                 995 
                 BLFS45 
                   Burkholderia  sp. 
                 2843 
                 2486 
                 75.2 
                 globlastp 
               
               
                 996 
                 BLFS45 
                   Burkholderia  sp. 
                 2844 
                 2486 
                 74.6 
                 globlastp 
               
               
                 1304 
                 BLFS100 
                 Environmental sample 
                 3139 
                 2536 
                 98.9 
                 globlastp 
               
               
                 1305 
                 BLFS100 
                 Environmental sample 
                 3140 
                 2536 
                 97.7 
                 globlastp 
               
               
                 1306 
                 BLFS100 
                   Pantoea  sp. 
                 3141 
                 2536 
                 96.6 
                 globlastp 
               
               
                 1307 
                 BLFS100 
                 Environmental sample 
                 3142 
                 2536 
                 95.4 
                 globlastp 
               
               
                 1308 
                 BLFS100 
                   Pantoea  sp. 
                 3143 
                 2536 
                 94.3 
                 globlastp 
               
               
                 1309 
                 BLFS100 
                   Pantoea  sp. 
                 3144 
                 2536 
                 88.5 
                 globlastp 
               
               
                 1310 
                 BLFS100 
                 Environmental sample 
                 3145 
                 2536 
                 87.4 
                 globlastp 
               
               
                 1311 
                 BLFS100 
                   Pantoea  sp. 
                 3146 
                 2536 
                 85.1 
                 globlastp 
               
               
                 1312 
                 BLFS100 
                   Pantoea  sp. 
                 3147 
                 2536 
                 83.9 
                 globlastp 
               
               
                 1313 
                 BLFS100 
                   Pantoea  sp. 
                 3148 
                 2536 
                 81.8 
                 globlastp 
               
               
                 1314 
                 BLFS100 
                   Pantoea  sp. 
                 3149 
                 2536 
                 80.7 
                 globlastp 
               
               
                 1315 
                 BLFS100 
                   Pantoea  sp. 
                 3150 
                 2536 
                 78.4 
                 globlastp 
               
               
                 1316 
                 BLFS100 
                   Pantoea  sp. 
                 3151 
                 2536 
                 76.1 
                 globlastp 
               
               
                 1317 
                 BLFS100 
                   Pantoea  sp. 
                 3152 
                 2536 
                 74.4 
                 globlastp 
               
               
                 1318 
                 BLFS100 
                   Plautia  sp. 
                 3153 
                 2536 
                 71 
                 globlastp 
               
               
                 1319 
                 BLFS100 
                   Erwinia  sp. 
                 3154 
                 2536 
                 70.1 
                 globlastp 
               
               
                 1218 
                 BLFS90 
                   Paenibacillus  sp. 
                 3053 
                 2526 
                 99.3 
                 globlastp 
               
               
                 1219 
                 BLFS90 
                   Paenibacillus  sp. 
                 3054 
                 2526 
                 98.9 
                 globlastp 
               
               
                 1220 
                 BLFS90 
                   Paenibacillus  sp. 
                 3055 
                 2526 
                 96.1 
                 globlastp 
               
               
                 1221 
                 BLFS90 
                   Paenibacillus  sp. 
                 3056 
                 2526 
                 95.4 
                 globlastp 
               
               
                 1222 
                 BLFS90 
                   Paenibacillus  sp. 
                 3057 
                 2526 
                 93.6 
                 globlastp 
               
               
                 1223 
                 BLFS90 
                   Paenibacillus  sp. 
                 3058 
                 2526 
                 92.1 
                 globlastp 
               
               
                 1224 
                 BLFS90 
                   Paenibacillus  sp. 
                 3059 
                 2526 
                 91.4 
                 globlastp 
               
               
                 1225 
                 BLFS90 
                   Paenibacillus  sp. 
                 3060 
                 2526 
                 90.7 
                 globlastp 
               
               
                 1226 
                 BLFS90 
                   Paenibacillus  sp. 
                 3061 
                 2526 
                 72.9 
                 globlastp 
               
               
                 1011 
                 BLFS55 
                   Janthinobacterium  sp. 
                 2857 
                 2495 
                 95.5 
                 globlastp 
               
               
                 1012 
                 BLFS55 
                   Duganella  sp. 
                 2858 
                 2495 
                 78.5 
                 globlastp 
               
               
                 2306 
                 ZLFS35 
                   Serratia  sp. 
                 4120 
                 2630 
                 99 
                 globlastp 
               
               
                 2307 
                 ZLFS35 
                   Serratia  sp. 
                 4121 
                 2630 
                 98.8 
                 globlastp 
               
               
                 2308 
                 ZLFS35 
                   Serratia  sp. 
                 4122 
                 2630 
                 98.1 
                 globlastp 
               
               
                 2309 
                 ZLFS35 
                   Serratia  sp. 
                 4123 
                 2630 
                 88.3 
                 globlastp 
               
               
                 2310 
                 ZLFS35 
                   Serratia  sp. 
                 4124 
                 2630 
                 73.2 
                 globlastp 
               
               
                 1152 
                 BLFS83 
                   Paenibacillus  sp. 
                 2989 
                 2519 
                 99 
                 globlastp 
               
               
                 1153 
                 BLFS83 
                   Paenibacillus  sp. 
                 2990 
                 2519 
                 97.4 
                 globlastp 
               
               
                 1154 
                 BLFS83 
                   Paenibacillus  sp. 
                 2991 
                 2519 
                 97 
                 globlastp 
               
               
                 1155 
                 BLFS83 
                   Paenibacillus  sp. 
                 2992 
                 2519 
                 96.4 
                 globlastp 
               
               
                 1156 
                 BLFS83 
                   Paenibacillus  sp. 
                 2993 
                 2519 
                 95 
                 globlastp 
               
               
                 1157 
                 BLFS83 
                   Paenibacillus  sp. 
                 2994 
                 2519 
                 94.3 
                 globlastp 
               
               
                 1158 
                 BLFS83 
                   Paenibacillus  sp. 
                 2995 
                 2519 
                 93.2 
                 globlastp 
               
               
                 1159 
                 BLFS83 
                   Paenibacillus  sp. 
                 2996 
                 2519 
                 92.3 
                 globlastp 
               
               
                 1160 
                 BLFS83 
                   Paenibacillus  sp. 
                 2997 
                 2519 
                 80.6 
                 globlastp 
               
               
                 1161 
                 BLFS83 
                   Paenibacillus  sp. 
                 2998 
                 2519 
                 80 
                 globlastp 
               
               
                 1162 
                 BLFS83 
                   Paenibacillus  sp. 
                 2999 
                 2519 
                 79.6 
                 globlastp 
               
               
                 870 
                 BLFS21 
                   Bacillus  sp. 
                 2722 
                 2464 
                 99.3 
                 globlastp 
               
               
                 871 
                 BLFS21 
                   Bacillus  sp. 
                 2723 
                 2464 
                 83.1 
                 globlastp 
               
               
                 872 
                 BLFS21 
                   Bacillus  sp. 
                 2724 
                 2464 
                 79.7 
                 globlastp 
               
               
                 873 
                 BLFS21 
                   Bacillus  sp. 
                 2725 
                 2464 
                 71.3 
                 globlastp 
               
               
                 874 
                 BLFS21 
                   Bacillus  sp. 
                 2726 
                 2464 
                 70.5 
                 globlastp 
               
               
                 875 
                 BLFS21 
                   Bacillus  sp. 
                 — 
                 2464 
                 70.23 
                 glotblastn 
               
               
                 876 
                 BLFS21 
                   Bacillus  sp. 
                 2727 
                 2464 
                 70.2 
                 globlastp 
               
               
                 877 
                 BLFS21 
                   Bacillus  sp. 
                 — 
                 2464 
                 70.14 
                 glotblastn 
               
               
                 1685 
                 BLFS129 
                   Pseudomonas  sp. 
                 3509 
                 2564 
                 95.4 
                 globlastp 
               
               
                 1686 
                 BLFS129 
                   Pseudomonas  sp. 
                 3510 
                 2564 
                 95 
                 globlastp 
               
               
                 1687 
                 BLFS129 
                   Pseudomonas  sp. 
                 3511 
                 2564 
                 94.5 
                 globlastp 
               
               
                 1688 
                 BLFS129 
                   Pseudomonas  sp. 
                 3512 
                 2564 
                 93.1 
                 globlastp 
               
               
                 1689 
                 BLFS129 
                   Pseudomonas  sp. 
                 3513 
                 2564 
                 91.3 
                 globlastp 
               
               
                 1690 
                 BLFS129 
                   Pseudomonas  sp. 
                 3514 
                 2564 
                 90.8 
                 globlastp 
               
               
                 1691 
                 BLFS129 
                 Environmental sample 
                 3515 
                 2564 
                 87.6 
                 globlastp 
               
               
                 1692 
                 BLFS129 
                   Pseudomonas  sp. 
                 3516 
                 2564 
                 83.9 
                 globlastp 
               
               
                 1693 
                 BLFS129 
                   Pseudomonas  sp. 
                 3517 
                 2564 
                 79.8 
                 globlastp 
               
               
                 1694 
                 BLFS129 
                   Pseudomonas  sp. 
                 3518 
                 2564 
                 76.6 
                 globlastp 
               
               
                 1695 
                 BLFS129 
                   Pseudomonas  sp. 
                 3519 
                 2564 
                 76.1 
                 globlastp 
               
               
                 920 
                 BLFS32 
                   Bacillus  sp. 
                 2770 
                 2474 
                 99 
                 globlastp 
               
               
                 921 
                 BLFS32 
                   Bacillus  sp. 
                 2771 
                 2474 
                 98 
                 globlastp 
               
               
                 922 
                 BLFS32 
                   Bacillus  sp. 
                 2772 
                 2474 
                 97.6 
                 globlastp 
               
               
                 923 
                 BLFS32 
                   Bacillus  sp. 
                 2773 
                 2474 
                 90.3 
                 globlastp 
               
               
                 924 
                 BLFS32 
                   Bacillus  sp. 
                 2774 
                 2474 
                 89.3 
                 globlastp 
               
               
                 925 
                 BLFS32 
                   Salinibacillus  sp. 
                 2775 
                 2474 
                 88.3 
                 globlastp 
               
               
                 926 
                 BLFS32 
                   Bacillus  sp. 
                 2776 
                 2474 
                 87.6 
                 globlastp 
               
               
                 927 
                 BLFS32 
                   Bacillus  sp. 
                 2777 
                 2474 
                 81.3 
                 globlastp 
               
               
                 928 
                 BLFS32 
                   Bacillus  sp. 
                 2778 
                 2474 
                 80.2 
                 globlastp 
               
               
                 929 
                 BLFS32 
                   Bacillus  sp. 
                 2779 
                 2474 
                 79.5 
                 globlastp 
               
               
                 930 
                 BLFS32 
                   Bacillus  sp. 
                 2780 
                 2474 
                 76.8 
                 globlastp 
               
               
                 931 
                 BLFS32 
                   Bacillus  sp. 
                 2781 
                 2474 
                 75.2 
                 globlastp 
               
               
                 932 
                 BLFS32 
                   Bacillus  sp. 
                 2782 
                 2474 
                 74.7 
                 globlastp 
               
               
                 933 
                 BLFS32 
                   Bacillus  sp. 
                 2783 
                 2474 
                 73.2 
                 globlastp 
               
               
                 934 
                 BLFS32 
                   Cohnella  sp. 
                 2784 
                 2474 
                 72.2 
                 globlastp 
               
               
                 935 
                 BLFS32 
                   Paenibacillus  sp. 
                 2785 
                 2474 
                 71.5 
                 globlastp 
               
               
                 936 
                 BLFS32 
                   Bacillus  sp. 
                 2786 
                 2474 
                 70.1 
                 globlastp 
               
               
                 1798 
                 BLFS138 
                   Bacillus  sp. 
                 3622 
                 2573 
                 98.7 
                 globlastp 
               
               
                 1799 
                 BLFS138 
                   Bacillus  sp. 
                 3623 
                 2573 
                 82.2 
                 globlastp 
               
               
                 1800 
                 BLFS138 
                   Bacillus  sp. 
                 3624 
                 2573 
                 81 
                 globlastp 
               
               
                 1801 
                 BLFS138 
                   Bacillus  sp. 
                 3625 
                 2573 
                 80.1 
                 globlastp 
               
               
                 1802 
                 BLFS138 
                   Bacillus  sp. 
                 3626 
                 2573 
                 79.4 
                 globlastp 
               
               
                 1803 
                 BLFS138 
                   Bacillus  sp. 
                 3627 
                 2573 
                 78.2 
                 globlastp 
               
               
                 1804 
                 BLFS138 
                   Bacillus  sp. 
                 3628 
                 2573 
                 77.1 
                 globlastp 
               
               
                 1805 
                 BLFS138 
                   Bacillus  sp. 
                 3629 
                 2573 
                 76.1 
                 globlastp 
               
               
                 1806 
                 BLFS138 
                   Bacillus  sp. 
                 3630 
                 2573 
                 74.3 
                 globlastp 
               
               
                 1807 
                 BLFS138 
                   Bacillus  sp. 
                 3631 
                 2573 
                 73.6 
                 globlastp 
               
               
                 1808 
                 BLFS138 
                   Bacillus  sp. 
                 3632 
                 2573 
                 72.5 
                 globlastp 
               
               
                 1809 
                 BLFS138 
                   Bacillus  sp. 
                 3633 
                 2573 
                 71.1 
                 globlastp 
               
               
                 1810 
                 BLFS138 
                   Bacillus  sp. 
                 — 
                 2573 
                 70 
                 glotblastn 
               
               
                 2103 
                 ZLFS18 
                 Environmental sample 
                 3917 
                 2615 
                 75.5 
                 globlastp 
               
               
                 1999 
                 BLFS158 
                   Bacillus  sp. 
                 3813 
                 2593 
                 73.8 
                 globlastp 
               
               
                 1857 
                 BLFS142 
                   Bacillus  sp. 
                 3680 
                 2577 
                 98.1 
                 globlastp 
               
               
                 1858 
                 BLFS142 
                   Bacillus  sp. 
                 3681 
                 2577 
                 96.7 
                 globlastp 
               
               
                 1859 
                 BLFS142 
                   Bacillus  sp. 
                 3682 
                 2577 
                 89 
                 globlastp 
               
               
                 1860 
                 BLFS142 
                   Bacillus  sp. 
                 3683 
                 2577 
                 88.5 
                 globlastp 
               
               
                 1861 
                 BLFS142 
                   Bacillus  sp. 
                 3684 
                 2577 
                 85.2 
                 globlastp 
               
               
                 1862 
                 BLFS142 
                   Bacillus  sp. 
                 3685 
                 2577 
                 84.2 
                 globlastp 
               
               
                 1863 
                 BLFS142 
                   Bacillus  sp. 
                 3686 
                 2577 
                 83.3 
                 globlastp 
               
               
                 1864 
                 BLFS142 
                   Bacillus  sp. 
                 3687 
                 2577 
                 82.3 
                 globlastp 
               
               
                 1865 
                 BLFS142 
                   Bacillus  sp. 
                 3688 
                 2577 
                 80.4 
                 globlastp 
               
               
                 1866 
                 BLFS142 
                   Bacillus  sp. 
                 3689 
                 2577 
                 79.3 
                 globlastp 
               
               
                 1867 
                 BLFS142 
                   Bacillus  sp. 
                 3690 
                 2577 
                 77.1 
                 globlastp 
               
               
                 1247 
                 BLFS93 
                   Paenibacillus  sp. 
                 3082 
                 2529 
                 98.9 
                 globlastp 
               
               
                 1248 
                 BLFS93 
                   Paenibacillus  sp. 
                 3083 
                 2529 
                 98.3 
                 globlastp 
               
               
                 1249 
                 BLFS93 
                   Paenibacillus  sp. 
                 3084 
                 2529 
                 97.7 
                 globlastp 
               
               
                 1250 
                 BLFS93 
                   Paenibacillus  sp. 
                 3085 
                 2529 
                 96 
                 globlastp 
               
               
                 1251 
                 BLFS93 
                   Paenibacillus  sp. 
                 3086 
                 2529 
                 95.4 
                 globlastp 
               
               
                 1252 
                 BLFS93 
                   Paenibacillus  sp. 
                 3087 
                 2529 
                 94.9 
                 globlastp 
               
               
                 1253 
                 BLFS93 
                   Paenibacillus  sp. 
                 3088 
                 2529 
                 93.1 
                 globlastp 
               
               
                 1254 
                 BLFS93 
                   Paenibacillus  sp. 
                 3089 
                 2529 
                 77 
                 globlastp 
               
               
                 1255 
                 BLFS93 
                   Paenibacillus  sp. 
                 3090 
                 2529 
                 75.9 
                 globlastp 
               
               
                 1256 
                 BLFS93 
                   Paenibacillus  sp. 
                 3091 
                 2529 
                 74.1 
                 globlastp 
               
               
                 1257 
                 BLFS93 
                   Paenibacillus  sp. 
                 3092 
                 2529 
                 72.6 
                 globlastp 
               
               
                 2341 
                 ZLFS38 
                   Serratia  sp. 
                 4154 
                 2633 
                 98.7 
                 globlastp 
               
               
                 2342 
                 ZLFS38 
                   Serratia  sp. 
                 4155 
                 2633 
                 96.2 
                 globlastp 
               
               
                 2343 
                 ZLFS38 
                   Serratia  sp. 
                 4156 
                 2633 
                 95 
                 globlastp 
               
               
                 2344 
                 ZLFS38 
                   Serratia  sp. 
                 4157 
                 2633 
                 94.3 
                 globlastp 
               
               
                 2345 
                 ZLFS38 
                   Serratia  sp. 
                 4158 
                 2633 
                 93.1 
                 globlastp 
               
               
                 2346 
                 ZLFS38 
                   Serratia  sp. 
                 4159 
                 2633 
                 92.5 
                 globlastp 
               
               
                 2347 
                 ZLFS38 
                   Pectobacterium  sp. 
                 4160 
                 2633 
                 89.3 
                 globlastp 
               
               
                 2348 
                 ZLFS38 
                   Hafnia  sp. 
                 4161 
                 2633 
                 88.1 
                 globlastp 
               
               
                 2349 
                 ZLFS38 
                   Dickeya  sp. 
                 4162 
                 2633 
                 87.4 
                 globlastp 
               
               
                 2350 
                 ZLFS38 
                   Dickeya  sp. 
                 4163 
                 2633 
                 86.2 
                 globlastp 
               
               
                 2351 
                 ZLFS38 
                   Enterobacter  sp. 
                 4164 
                 2633 
                 85 
                 globlastp 
               
               
                 2352 
                 ZLFS38 
                   Achromobacter  sp. 
                 4165 
                 2633 
                 84.3 
                 globlastp 
               
               
                 2353 
                 ZLFS38 
                 Environmental sample 
                 4166 
                 2633 
                 83 
                 globlastp 
               
               
                 2354 
                 ZLFS38 
                   Serratia  sp. 
                 4167 
                 2633 
                 82.2 
                 globlastp 
               
               
                 2355 
                 ZLFS38 
                 Environmental sample 
                 4168 
                 2633 
                 81.1 
                 globlastp 
               
               
                 2356 
                 ZLFS38 
                 Environmental sample 
                 4169 
                 2633 
                 80 
                 globlastp 
               
               
                 2357 
                 ZLFS38 
                   Cronobacter  sp. 
                 4170 
                 2633 
                 79.5 
                 globlastp 
               
               
                 2358 
                 ZLFS38 
                   Enterobacter  sp. 
                 4171 
                 2633 
                 78.1 
                 globlastp 
               
               
                 2359 
                 ZLFS38 
                   Arsenophonus  sp. 
                 4172 
                 2633 
                 77.4 
                 globlastp 
               
               
                 2360 
                 ZLFS38 
                   Pantoea  sp. 
                 4173 
                 2633 
                 76.1 
                 globlastp 
               
               
                 2361 
                 ZLFS38 
                   Vibrio  sp. 
                 4174 
                 2633 
                 72 
                 globlastp 
               
               
                 2362 
                 ZLFS38 
                   Sodalis  sp. 
                 4175 
                 2633 
                 71 
                 globlastp 
               
               
                 2363 
                 ZLFS38 
                   Vibrio  sp. 
                 4176 
                 2633 
                 70 
                 globlastp 
               
               
                 803 
                 BLFS9 
                   Amycolatopsis  sp. 
                 2655 
                 2452 
                 92.3 
                 globlastp 
               
               
                 804 
                 BLFS9 
                   Amycolatopsis  sp. 
                 2656 
                 2452 
                 78.6 
                 globlastp 
               
               
                 805 
                 BLFS9 
                   Streptomyces  sp. 
                 2657 
                 2452 
                 71.8 
                 globlastp 
               
               
                 806 
                 BLFS9 
                   Streptomyces  sp. 
                 2658 
                 2452 
                 71.4 
                 globlastp 
               
               
                 807 
                 BLFS9 
                   Streptomyces  sp. 
                 2659 
                 2452 
                 70.9 
                 globlastp 
               
               
                 808 
                 BLFS9 
                   Streptomyces  sp. 
                 2660 
                 2452 
                 70.1 
                 globlastp 
               
               
                 1090 
                 BLFS74 
                   Paenibacillus  sp. 
                 2930 
                 2512 
                 99.4 
                 globlastp 
               
               
                 1091 
                 BLFS74 
                   Paenibacillus  sp. 
                 2931 
                 2512 
                 99.1 
                 globlastp 
               
               
                 1092 
                 BLFS74 
                   Paenibacillus  sp. 
                 2932 
                 2512 
                 98.6 
                 globlastp 
               
               
                 1093 
                 BLFS74 
                   Paenibacillus  sp. 
                 2933 
                 2512 
                 97.4 
                 globlastp 
               
               
                 1094 
                 BLFS74 
                   Paenibacillus  sp. 
                 2934 
                 2512 
                 97.1 
                 globlastp 
               
               
                 1095 
                 BLFS74 
                   Paenibacillus  sp. 
                 2935 
                 2512 
                 96 
                 globlastp 
               
               
                 1096 
                 BLFS74 
                   Paenibacillus  sp. 
                 2936 
                 2512 
                 95.7 
                 globlastp 
               
               
                 1097 
                 BLFS74 
                   Paenibacillus  sp. 
                 2937 
                 2512 
                 95.4 
                 globlastp 
               
               
                 1098 
                 BLFS74 
                   Paenibacillus  sp. 
                 2938 
                 2512 
                 92.2 
                 globlastp 
               
               
                 1099 
                 BLFS74 
                   Paenibacillus  sp. 
                 2939 
                 2512 
                 91.7 
                 globlastp 
               
               
                 1100 
                 BLFS74 
                   Paenibacillus  sp. 
                 2940 
                 2512 
                 90.5 
                 globlastp 
               
               
                 1101 
                 BLFS74 
                   Paenibacillus  sp. 
                 2941 
                 2512 
                 89.7 
                 globlastp 
               
               
                 1102 
                 BLFS74 
                   Paenibacillus  sp. 
                 2942 
                 2512 
                 89.1 
                 globlastp 
               
               
                 1103 
                 BLFS74 
                   Paenibacillus  sp. 
                 2943 
                 2512 
                 87.1 
                 globlastp 
               
               
                 2140 
                 ZLFS23 
                   Curtobacterium  sp. 
                 3954 
                 2619 
                 95.8 
                 globlastp 
               
               
                 2141 
                 ZLFS23 
                   Curtobacterium  sp. 
                 3955 
                 2619 
                 95.4 
                 globlastp 
               
               
                 2142 
                 ZLFS23 
                   Curtobacterium  sp. 
                 3956 
                 2619 
                 90.5 
                 globlastp 
               
               
                 2143 
                 ZLFS23 
                   Curtobacterium  sp. 
                 3957 
                 2619 
                 80.5 
                 globlastp 
               
               
                 2144 
                 ZLFS23 
                 Environmental sample 
                 3958 
                 2619 
                 76.3 
                 globlastp 
               
               
                 2145 
                 ZLFS23 
                 Environmental sample 
                 3959 
                 2619 
                 74.5 
                 globlastp 
               
               
                 2146 
                 ZLFS23 
                 Environmental sample 
                 3960 
                 2619 
                 71.8 
                 globlastp 
               
               
                 1420 
                 BLFS108 
                   Pantoea  sp. 
                 3246 
                 2544 
                 99.1 
                 globlastp 
               
               
                 1421 
                 BLFS108 
                 Environmental sample 
                 3247 
                 2544 
                 98.3 
                 globlastp 
               
               
                 1422 
                 BLFS108 
                   Klebsiella  sp. 
                 3248 
                 2544 
                 76.9 
                 globlastp 
               
               
                 1423 
                 BLFS108 
                   Citrobacter  sp. 
                 3249 
                 2544 
                 76.1 
                 globlastp 
               
               
                 1424 
                 BLFS108 
                   Meta  sp. 
                 3250 
                 2544 
                 75.2 
                 globlastp 
               
               
                 1425 
                 BLFS108 
                 Environmental sample 
                 3251 
                 2544 
                 74.4 
                 globlastp 
               
               
                 1426 
                 BLFS108 
                   Salmonella  sp. 
                 3252 
                 2544 
                 73.5 
                 globlastp 
               
               
                 1427 
                 BLFS108 
                   Escherichia  sp. 
                 3253 
                 2544 
                 72 
                 globlastp 
               
               
                 1428 
                 BLFS108 
                   Kluyvera  sp. 
                 3254 
                 2544 
                 71.8 
                 globlastp 
               
               
                 2311 
                 ZLFS36 
                   Serratia  sp. 
                 4125 
                 2631 
                 99.2 
                 globlastp 
               
               
                 2170 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3984 
                 2622 
                 93 
                 globlastp 
               
               
                 2171 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3985 
                 2622 
                 92 
                 globlastp 
               
               
                 2172 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3986 
                 2622 
                 91 
                 globlastp 
               
               
                 2173 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3987 
                 2622 
                 90.8 
                 globlastp 
               
               
                 2174 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3988 
                 2622 
                 89 
                 globlastp 
               
               
                 2175 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3989 
                 2622 
                 88 
                 globlastp 
               
               
                 2176 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3990 
                 2622 
                 87.1 
                 globlastp 
               
               
                 2177 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3991 
                 2622 
                 86.1 
                 globlastp 
               
               
                 2178 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3992 
                 2622 
                 85.1 
                 globlastp 
               
               
                 2179 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3993 
                 2622 
                 84.1 
                 globlastp 
               
               
                 2180 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3994 
                 2622 
                 83.1 
                 globlastp 
               
               
                 2181 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3995 
                 2622 
                 82.4 
                 globlastp 
               
               
                 2182 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3996 
                 2622 
                 81.9 
                 globlastp 
               
               
                 2183 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3997 
                 2622 
                 79.1 
                 globlastp 
               
               
                 2184 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3998 
                 2622 
                 78 
                 globlastp 
               
               
                 2185 
                 ZLFS27 
                   Pseudomonas  sp. 
                 3999 
                 2622 
                 77.1 
                 globlastp 
               
               
                 2186 
                 ZLFS27 
                   Pseudomonas  sp. 
                 4000 
                 2622 
                 75.9 
                 globlastp 
               
               
                 2187 
                 ZLFS27 
                   Pseudomonas  sp. 
                 4001 
                 2622 
                 75.3 
                 globlastp 
               
               
                 2188 
                 ZLFS27 
                   Pseudomonas  sp. 
                 4002 
                 2622 
                 74.2 
                 globlastp 
               
               
                 2189 
                 ZLFS27 
                   Pseudomonas  sp. 
                 4003 
                 2622 
                 73.8 
                 globlastp 
               
               
                 2190 
                 ZLFS27 
                 Environmental sample 
                 4004 
                 2622 
                 72 
                 globlastp 
               
               
                 2191 
                 ZLFS27 
                   Pseudomonas  sp. 
                 4005 
                 2622 
                 71.5 
                 globlastp 
               
               
                 2192 
                 ZLFS27 
                   Pseudomonas  sp. 
                 4006 
                 2622 
                 70.3 
                 globlastp 
               
               
                 2193 
                 ZLFS27 
                   Pseudomonas  sp. 
                 4007 
                 2622 
                 70.1 
                 globlastp 
               
               
                 1824 
                 BLFS140 
                   Salinibacillus  sp. 
                 3647 
                 2575 
                 99.4 
                 globlastp 
               
               
                 1825 
                 BLFS140 
                   Bacillus  sp. 
                 3648 
                 2575 
                 98.4 
                 globlastp 
               
               
                 1826 
                 BLFS140 
                   Bacillus  sp. 
                 3649 
                 2575 
                 95.6 
                 globlastp 
               
               
                 1827 
                 BLFS140 
                   Bacillus  sp. 
                 3650 
                 2575 
                 88 
                 globlastp 
               
               
                 1828 
                 BLFS140 
                   Bacillus  sp. 
                 3651 
                 2575 
                 87.1 
                 globlastp 
               
               
                 1829 
                 BLFS140 
                   Bacillus  sp. 
                 3652 
                 2575 
                 86.1 
                 globlastp 
               
               
                 1830 
                 BLFS140 
                   Bacillus  sp. 
                 3653 
                 2575 
                 85.2 
                 globlastp 
               
               
                 1831 
                 BLFS140 
                   Bacillus  sp. 
                 3654 
                 2575 
                 85 
                 globlastp 
               
               
                 1832 
                 BLFS140 
                   Bacillus  sp. 
                 3655 
                 2575 
                 84.9 
                 globlastp 
               
               
                 1833 
                 BLFS140 
                   Bacillus  sp. 
                 3656 
                 2575 
                 84.7 
                 globlastp 
               
               
                 1834 
                 BLFS140 
                   Bacillus  sp. 
                 3657 
                 2575 
                 81.6 
                 globlastp 
               
               
                 1835 
                 BLFS140 
                   Bacillus  sp. 
                 3658 
                 2575 
                 81.5 
                 globlastp 
               
               
                 1836 
                 BLFS140 
                   Bacillus  sp. 
                 3659 
                 2575 
                 74 
                 globlastp 
               
               
                 1837 
                 BLFS140 
                   Bacillus  sp. 
                 3660 
                 2575 
                 73.8 
                 globlastp 
               
               
                 2102 
                 ZLFS48 
                 Environmental sample 
                 3916 
                 2639 
                 79.4 
                 globlastp 
               
               
                 982 
                 BLFS42 
                   Burkholderia  sp. 
                 2830 
                 2483 
                 97.6 
                 globlastp 
               
               
                 983 
                 BLFS42 
                   Burkholderia  sp. 
                 2831 
                 2483 
                 97.2 
                 globlastp 
               
               
                 1927 
                 BLFS147 
                   Salinibacillus  sp. 
                 3750 
                 2582 
                 98.9 
                 globlastp 
               
               
                 1928 
                 BLFS147 
                   Bacillus  sp. 
                 3751 
                 2582 
                 97.7 
                 globlastp 
               
               
                 1929 
                 BLFS147 
                   Bacillus  sp. 
                 3752 
                 2582 
                 95.7 
                 globlastp 
               
               
                 1930 
                 BLFS147 
                   Bacillus  sp. 
                 3753 
                 2582 
                 88 
                 globlastp 
               
               
                 1931 
                 BLFS147 
                   Bacillus  sp. 
                 3754 
                 2582 
                 87.3 
                 globlastp 
               
               
                 1932 
                 BLFS147 
                   Bacillus  sp. 
                 3755 
                 2582 
                 86 
                 globlastp 
               
               
                 1933 
                 BLFS147 
                   Bacillus  sp. 
                 3756 
                 2582 
                 85 
                 globlastp 
               
               
                 1934 
                 BLFS147 
                   Bacillus  sp. 
                 3757 
                 2582 
                 84.8 
                 globlastp 
               
               
                 1935 
                 BLFS147 
                   Bacillus  sp. 
                 3758 
                 2582 
                 74.1 
                 globlastp 
               
               
                 1936 
                 BLFS147 
                   Bacillus  sp. 
                 3759 
                 2582 
                 73.9 
                 globlastp 
               
               
                 1170 
                 BLFS85 
                   Paenibacillus  sp. 
                 3007 
                 2521 
                 99.4 
                 globlastp 
               
               
                 1171 
                 BLFS85 
                   Paenibacillus  sp. 
                 3008 
                 2521 
                 98.3 
                 globlastp 
               
               
                 1172 
                 BLFS85 
                   Paenibacillus  sp. 
                 3009 
                 2521 
                 97.6 
                 globlastp 
               
               
                 1173 
                 BLFS85 
                   Paenibacillus  sp. 
                 3010 
                 2521 
                 97 
                 globlastp 
               
               
                 1174 
                 BLFS85 
                   Paenibacillus  sp. 
                 3011 
                 2521 
                 96.6 
                 globlastp 
               
               
                 1175 
                 BLFS85 
                   Paenibacillus  sp. 
                 3012 
                 2521 
                 95.3 
                 globlastp 
               
               
                 1176 
                 BLFS85 
                   Paenibacillus  sp. 
                 3013 
                 2521 
                 93.2 
                 globlastp 
               
               
                 1177 
                 BLFS85 
                   Paenibacillus  sp. 
                 3014 
                 2521 
                 92.7 
                 globlastp 
               
               
                 1178 
                 BLFS85 
                   Paenibacillus  sp. 
                 3015 
                 2521 
                 91 
                 globlastp 
               
               
                 1179 
                 BLFS85 
                   Paenibacillus  sp. 
                 3016 
                 2521 
                 90.4 
                 globlastp 
               
               
                 1180 
                 BLFS85 
                   Paenibacillus  sp. 
                 3017 
                 2521 
                 89.1 
                 globlastp 
               
               
                 1552 
                 BLFS118 
                   Pseudomonas  sp. 
                 3377 
                 2553 
                 98.8 
                 globlastp 
               
               
                 1553 
                 BLFS118 
                   Pseudomonas  sp. 
                 3378 
                 2553 
                 98.5 
                 globlastp 
               
               
                 1554 
                 BLFS118 
                   Pseudomonas  sp. 
                 3379 
                 2553 
                 98.2 
                 globlastp 
               
               
                 1555 
                 BLFS118 
                   Pseudomonas  sp. 
                 3380 
                 2553 
                 97.7 
                 globlastp 
               
               
                 1556 
                 BLFS118 
                   Pseudomonas  sp. 
                 3381 
                 2553 
                 97.1 
                 globlastp 
               
               
                 1557 
                 BLFS118 
                   Pseudomonas  sp. 
                 3382 
                 2553 
                 96.8 
                 globlastp 
               
               
                 1558 
                 BLFS118 
                   Pseudomonas  sp. 
                 3383 
                 2553 
                 96.2 
                 globlastp 
               
               
                 1559 
                 BLFS118 
                   Pseudomonas  sp. 
                 3384 
                 2553 
                 88 
                 globlastp 
               
               
                 1560 
                 BLFS118 
                   Pseudomonas  sp. 
                 3385 
                 2553 
                 85.9 
                 globlastp 
               
               
                 1561 
                 BLFS118 
                   Pseudomonas  sp. 
                 3386 
                 2553 
                 78 
                 globlastp 
               
               
                 1562 
                 BLFS118 
                   Pseudomonas  sp. 
                 3387 
                 2553 
                 77.1 
                 globlastp 
               
               
                 1563 
                 BLFS118 
                   Pseudomonas  sp. 
                 3388 
                 2553 
                 76 
                 globlastp 
               
               
                 1564 
                 BLFS118 
                 Environmental sample 
                 3389 
                 2553 
                 75.1 
                 globlastp 
               
               
                 1565 
                 BLFS118 
                   Pseudomonas  sp. 
                 3390 
                 2553 
                 74 
                 globlastp 
               
               
                 1566 
                 BLFS118 
                   Pseudomonas  sp. 
                 3391 
                 2553 
                 73 
                 globlastp 
               
               
                 1567 
                 BLFS118 
                   Pseudomonas  sp. 
                 3392 
                 2553 
                 72.1 
                 globlastp 
               
               
                 1568 
                 BLFS118 
                 Environmental sample 
                 3393 
                 2553 
                 71 
                 globlastp 
               
               
                 1569 
                 BLFS118 
                   Pseudomonas  sp. 
                 3394 
                 2553 
                 70.1 
                 globlastp 
               
               
                 1357 
                 BLFS104 
                   Pantoea  sp. 
                 3183 
                 2540 
                 99.2 
                 globlastp 
               
               
                 1358 
                 BLFS104 
                   Pantoea  sp. 
                 3184 
                 2540 
                 98.3 
                 globlastp 
               
               
                 1359 
                 BLFS104 
                 Environmental sample 
                 3185 
                 2540 
                 95 
                 globlastp 
               
               
                 1360 
                 BLFS104 
                   Pantoea  sp. 
                 3186 
                 2540 
                 94.1 
                 globlastp 
               
               
                 1361 
                 BLFS104 
                   Pantoea  sp. 
                 3187 
                 2540 
                 93.3 
                 globlastp 
               
               
                 1362 
                 BLFS104 
                   Pantoea  sp. 
                 3188 
                 2540 
                 90.8 
                 globlastp 
               
               
                 1363 
                 BLFS104 
                 Environmental sample 
                 3189 
                 2540 
                 89.1 
                 globlastp 
               
               
                 1364 
                 BLFS104 
                   Pantoea  sp. 
                 3190 
                 2540 
                 84.9 
                 globlastp 
               
               
                 1365 
                 BLFS104 
                   Pantoea  sp. 
                 3191 
                 2540 
                 78.2 
                 globlastp 
               
               
                 1366 
                 BLFS104 
                 Environmental sample 
                 3192 
                 2540 
                 77.3 
                 globlastp 
               
               
                 1367 
                 BLFS104 
                   Pantoea  sp. 
                 3193 
                 2540 
                 73.9 
                 globlastp 
               
               
                 1381 
                 BLFS106 
                 Environmental sample 
                 3207 
                 2542 
                 99 
                 globlastp 
               
               
                 1382 
                 BLFS106 
                   Pantoea  sp. 
                 3208 
                 2542 
                 98 
                 globlastp 
               
               
                 1383 
                 BLFS106 
                   Pantoea  sp. 
                 3209 
                 2542 
                 97 
                 globlastp 
               
               
                 1384 
                 BLFS106 
                   Pantoea  sp. 
                 3210 
                 2542 
                 94.4 
                 globlastp 
               
               
                 1385 
                 BLFS106 
                 Environmental sample 
                 3211 
                 2542 
                 92.1 
                 globlastp 
               
               
                 1386 
                 BLFS106 
                   Pantoea  sp. 
                 3212 
                 2542 
                 88.1 
                 globlastp 
               
               
                 1387 
                 BLFS106 
                   Pantoea  sp. 
                 3213 
                 2542 
                 86.9 
                 globlastp 
               
               
                 1388 
                 BLFS106 
                   Pantoea  sp. 
                 3214 
                 2542 
                 83.2 
                 globlastp 
               
               
                 1389 
                 BLFS106 
                   Pantoea  sp. 
                 3215 
                 2542 
                 82.2 
                 globlastp 
               
               
                 1390 
                 BLFS106 
                   Pantoea  sp. 
                 3216 
                 2542 
                 80.2 
                 globlastp 
               
               
                 1391 
                 BLFS106 
                 Environmental sample 
                 3217 
                 2542 
                 79.2 
                 globlastp 
               
               
                 1392 
                 BLFS106 
                   Pantoea  sp. 
                 3218 
                 2542 
                 78.2 
                 globlastp 
               
               
                 1393 
                 BLFS106 
                   Plautia  sp. 
                 3219 
                 2542 
                 78.1 
                 globlastp 
               
               
                 1394 
                 BLFS106 
                   Pantoea  sp. 
                 3220 
                 2542 
                 77.2 
                 globlastp 
               
               
                 1395 
                 BLFS106 
                   Cedecea  sp. 
                 3221 
                 2542 
                 76.2 
                 globlastp 
               
               
                 1396 
                 BLFS106 
                   Enterobacter  sp. 
                 3222 
                 2542 
                 75.2 
                 globlastp 
               
               
                 1397 
                 BLFS106 
                 Environmental sample 
                 3223 
                 2542 
                 74.3 
                 globlastp 
               
               
                 1398 
                 BLFS106 
                 Environmental sample 
                 3224 
                 2542 
                 73.3 
                 globlastp 
               
               
                 1399 
                 BLFS106 
                 Environmental sample 
                 3225 
                 2542 
                 72.3 
                 globlastp 
               
               
                 1400 
                 BLFS106 
                   Citrobacter  sp. 
                 3226 
                 2542 
                 71.3 
                 globlastp 
               
               
                 1401 
                 BLFS106 
                   Enterobacter  sp. 
                 3227 
                 2542 
                 70.3 
                 globlastp 
               
               
                 1238 
                 BLFS92 
                   Paenibacillus  sp. 
                 3073 
                 2528 
                 99.2 
                 globlastp 
               
               
                 1239 
                 BLFS92 
                   Paenibacillus  sp. 
                 3074 
                 2528 
                 97.7 
                 globlastp 
               
               
                 1240 
                 BLFS92 
                   Paenibacillus  sp. 
                 3075 
                 2528 
                 97.2 
                 globlastp 
               
               
                 1241 
                 BLFS92 
                   Paenibacillus  sp. 
                 3076 
                 2528 
                 96.6 
                 globlastp 
               
               
                 1242 
                 BLFS92 
                   Paenibacillus  sp. 
                 3077 
                 2528 
                 96.1 
                 globlastp 
               
               
                 1243 
                 BLFS92 
                   Paenibacillus  sp. 
                 3078 
                 2528 
                 94.3 
                 globlastp 
               
               
                 1244 
                 BLFS92 
                   Paenibacillus  sp. 
                 3079 
                 2528 
                 94 
                 globlastp 
               
               
                 1245 
                 BLFS92 
                   Paenibacillus  sp. 
                 3080 
                 2528 
                 93.5 
                 globlastp 
               
               
                 1246 
                 BLFS92 
                   Paenibacillus  sp. 
                 3081 
                 2528 
                 92 
                 globlastp 
               
               
                 1868 
                 BLFS143 
                   Salinibacillus  sp. 
                 3691 
                 2578 
                 99.4 
                 globlastp 
               
               
                 1869 
                 BLFS143 
                   Bacillus  sp. 
                 3692 
                 2578 
                 98.2 
                 globlastp 
               
               
                 1870 
                 BLFS143 
                   Bacillus  sp. 
                 3693 
                 2578 
                 96.1 
                 globlastp 
               
               
                 1871 
                 BLFS143 
                   Bacillus  sp. 
                 3694 
                 2578 
                 95.2 
                 globlastp 
               
               
                 1872 
                 BLFS143 
                   Bacillus  sp. 
                 3695 
                 2578 
                 94.6 
                 globlastp 
               
               
                 1873 
                 BLFS143 
                   Bacillus  sp. 
                 3696 
                 2578 
                 87.5 
                 globlastp 
               
               
                 1874 
                 BLFS143 
                   Bacillus  sp. 
                 3697 
                 2578 
                 86.9 
                 globlastp 
               
               
                 1875 
                 BLFS143 
                   Bacillus  sp. 
                 3698 
                 2578 
                 79.1 
                 globlastp 
               
               
                 1876 
                 BLFS143 
                   Bacillus  sp. 
                 3699 
                 2578 
                 78.5 
                 globlastp 
               
               
                 1877 
                 BLFS143 
                   Bacillus  sp. 
                 3700 
                 2578 
                 74.1 
                 globlastp 
               
               
                 1878 
                 BLFS143 
                   Bacillus  sp. 
                 3701 
                 2578 
                 72.9 
                 globlastp 
               
               
                 1879 
                 BLFS143 
                   Bacillus  sp. 
                 3702 
                 2578 
                 71.3 
                 globlastp 
               
               
                 1880 
                 BLFS143 
                   Bacillus  sp. 
                 3703 
                 2578 
                 70.4 
                 globlastp 
               
               
                 1881 
                 BLFS143 
                   Bacillus  sp. 
                 3704 
                 2578 
                 70.1 
                 globlastp 
               
               
                 800 
                 BLFS8 
                   Amycolatopsis  sp. 
                 2652 
                 2451 
                 98.3 
                 globlastp 
               
               
                 801 
                 BLFS8 
                   Amycolatopsis  sp. 
                 2653 
                 2451 
                 95.7 
                 globlastp 
               
               
                 802 
                 BLFS8 
                   Amycolatopsis  sp. 
                 2654 
                 2451 
                 77.1 
                 globlastp 
               
               
                 961 
                 BLFS36 
                   Bradyrhizobium  sp. 
                 2811 
                 2478 
                 82.9 
                 globlastp 
               
               
                 962 
                 BLFS36 
                   Bradyrhizobium  sp. 
                 2812 
                 2478 
                 78.3 
                 globlastp 
               
               
                 963 
                 BLFS36 
                   Bradyrhizobium  sp. 
                 — 
                 2478 
                 76.83 
                 glotblastn 
               
               
                 964 
                 BLFS36 
                   Bradyrhizobium  sp. 
                 2813 
                 2478 
                 76.8 
                 globlastp 
               
               
                 965 
                 BLFS36 
                   Bradyrhizobium  sp. 
                 2814 
                 2478 
                 75.6 
                 globlastp 
               
               
                 966 
                 BLFS36 
                   Bradyrhizobium  sp. 
                 2815 
                 2478 
                 74.4 
                 globlastp 
               
               
                 1402 
                 BLFS107 
                 Environmental sample 
                 3228 
                 2543 
                 97.9 
                 globlastp 
               
               
                 1403 
                 BLFS107 
                   Pantoea  sp. 
                 3229 
                 2543 
                 97.4 
                 globlastp 
               
               
                 1404 
                 BLFS107 
                   Pantoea  sp. 
                 3230 
                 2543 
                 96.8 
                 globlastp 
               
               
                 1405 
                 BLFS107 
                 Environmental sample 
                 3231 
                 2543 
                 95.8 
                 globlastp 
               
               
                 1406 
                 BLFS107 
                   Pantoea  sp. 
                 3232 
                 2543 
                 94.2 
                 globlastp 
               
               
                 1407 
                 BLFS107 
                   Pantoea  sp. 
                 3233 
                 2543 
                 88.4 
                 globlastp 
               
               
                 1408 
                 BLFS107 
                 Environmental sample 
                 3234 
                 2543 
                 86.3 
                 globlastp 
               
               
                 1409 
                 BLFS107 
                 Environmental sample 
                 3235 
                 2543 
                 85.8 
                 globlastp 
               
               
                 1410 
                 BLFS107 
                 Environmental sample 
                 3236 
                 2543 
                 85.7 
                 globlastp 
               
               
                 1411 
                 BLFS107 
                 Environmental sample 
                 3237 
                 2543 
                 85.3 
                 globlastp 
               
               
                 1412 
                 BLFS107 
                   Pantoea  sp. 
                 3238 
                 2543 
                 84.8 
                 globlastp 
               
               
                 1413 
                 BLFS107 
                   Pantoea  sp. 
                 3239 
                 2543 
                 84.7 
                 globlastp 
               
               
                 1414 
                 BLFS107 
                   Pantoea  sp. 
                 3240 
                 2543 
                 78.3 
                 globlastp 
               
               
                 1415 
                 BLFS107 
                   Erwinia  sp. 
                 3241 
                 2543 
                 74.6 
                 globlastp 
               
               
                 1416 
                 BLFS107 
                   Erwinia  sp. 
                 3242 
                 2543 
                 73.5 
                 globlastp 
               
               
                 1417 
                 BLFS107 
                   Erwinia  sp. 
                 3243 
                 2543 
                 72.3 
                 globlastp 
               
               
                 1418 
                 BLFS107 
                   Pantoea  sp. 
                 3244 
                 2543 
                 72 
                 globlastp 
               
               
                 1419 
                 BLFS107 
                 Environmental sample 
                 3245 
                 2543 
                 70.4 
                 globlastp 
               
               
                 866 
                 BLFS20 
                   Bacillus  sp. 
                 2718 
                 2463 
                 99.1 
                 globlastp 
               
               
                 867 
                 BLFS20 
                   Bacillus  sp. 
                 2719 
                 2463 
                 98.2 
                 globlastp 
               
               
                 868 
                 BLFS20 
                   Bacillus  sp. 
                 2720 
                 2463 
                 97.2 
                 globlastp 
               
               
                 869 
                 BLFS20 
                   Bacillus  sp. 
                 2721 
                 2463 
                 96.3 
                 globlastp 
               
               
                 2012 
                 PUB65 
                   Variovorax  sp. 
                 3826 
                 2598 
                 98.9 
                 globlastp 
               
               
                 2013 
                 PUB65 
                   Variovorax  sp. 
                 3827 
                 2598 
                 94.8 
                 globlastp 
               
               
                 2014 
                 PUB65 
                   Variovorax  sp. 
                 3828 
                 2598 
                 94.5 
                 globlastp 
               
               
                 2015 
                 PUB65 
                   Variovorax  sp. 
                 3829 
                 2598 
                 91.4 
                 globlastp 
               
               
                 2016 
                 PUB65 
                   Variovorax  sp. 
                 3830 
                 2598 
                 91.3 
                 globlastp 
               
               
                 2017 
                 PUB65 
                   Variovorax  sp. 
                 3831 
                 2598 
                 90.6 
                 globlastp 
               
               
                 2018 
                 PUB65 
                   Variovorax  sp. 
                 3832 
                 2598 
                 90.5 
                 globlastp 
               
               
                 2019 
                 PUB65 
                   Variovorax  sp. 
                 3833 
                 2598 
                 90.3 
                 globlastp 
               
               
                 2020 
                 PUB65 
                 Environmental sample 
                 3834 
                 2598 
                 90.1 
                 globlastp 
               
               
                 2021 
                 PUB65 
                 Environmental sample 
                 3835 
                 2598 
                 87.1 
                 globlastp 
               
               
                 2022 
                 PUB65 
                   Variovorax  sp. 
                 3836 
                 2598 
                 84.5 
                 globlastp 
               
               
                 2023 
                 PUB65 
                   Variovorax  sp. 
                 3837 
                 2598 
                 84.3 
                 globlastp 
               
               
                 2024 
                 PUB65 
                 Environmental sample 
                 3838 
                 2598 
                 78.4 
                 globlastp 
               
               
                 2025 
                 PUB65 
                 Environmental sample 
                 3839 
                 2598 
                 77.9 
                 globlastp 
               
               
                 2026 
                 PUB65 
                   Variovorax  sp. 
                 3840 
                 2598 
                 77.4 
                 globlastp 
               
               
                 2027 
                 PUB65 
                   Acidovorax  sp. 
                 3841 
                 2598 
                 75.5 
                 globlastp 
               
               
                 2028 
                 PUB65 
                 Environmental sample 
                 3842 
                 2598 
                 75.4 
                 globlastp 
               
               
                 2029 
                 PUB65 
                   Acidovorax  sp. 
                 3843 
                 2598 
                 75.2 
                 globlastp 
               
               
                 2030 
                 PUB65 
                 Environmental sample 
                 3844 
                 2598 
                 74.5 
                 globlastp 
               
               
                 2031 
                 PUB65 
                 Environmental sample 
                 3845 
                 2598 
                 72.3 
                 globlastp 
               
               
                 790 
                 BLFS1 
                   Streptomyces  sp. 
                 2642 
                 2444 
                 72.8 
                 globlastp 
               
               
                 1976 
                 BLFS150 
                   Pseudomonas  sp. 
                 — 
                 2585 
                 95.9 
                 glotblastn 
               
               
                 1977 
                 BLFS150 
                   Xanthomonas  sp. 
                 — 
                 2585 
                 77.05 
                 glotblastn 
               
               
                 1978 
                 BLFS150 
                   Xanthomonas  sp. 
                 — 
                 2585 
                 75.41 
                 glotblastn 
               
               
                 1979 
                 BLFS150 
                   Xanthomonas  sp. 
                 — 
                 2585 
                 72.95 
                 glotblastn 
               
               
                 1980 
                 BLFS150 
                   Xanthomonas  sp. 
                 — 
                 2585 
                 71.31 
                 glotblastn 
               
               
                 2081 
                 ZLFS8 
                   Collimonas  sp. 
                 3895 
                 2605 
                 78.5 
                 globlastp 
               
               
                 2082 
                 ZLFS8 
                   Collimonas  sp. 
                 3896 
                 2605 
                 78.3 
                 globlastp 
               
               
                 952 
                 BLFS35 
                   Bradyrhizohium  sp. 
                 2802 
                 2477 
                 96.3 
                 globlastp 
               
               
                 953 
                 BLFS35 
                   Mesorhizohium  sp. 
                 2803 
                 2477 
                 89.8 
                 globlastp 
               
               
                 954 
                 BLFS35 
                   Bradyrhizohium  sp. 
                 2804 
                 2477 
                 87 
                 globlastp 
               
               
                 955 
                 BLFS35 
                   Bradyrhizohium  sp. 
                 2805 
                 2477 
                 86 
                 globlastp 
               
               
                 956 
                 BLFS35 
                   Bradyrhizohium  sp. 
                 2806 
                 2477 
                 76.8 
                 globlastp 
               
               
                 957 
                 BLFS35 
                   Bradyrhizohium  sp. 
                 2807 
                 2477 
                 75.5 
                 globlastp 
               
               
                 958 
                 BLFS35 
                   Bradyrhizohium  sp. 
                 2808 
                 2477 
                 75 
                 globlastp 
               
               
                 959 
                 BLFS35 
                   Bradyrhizohium  sp. 
                 2809 
                 2477 
                 71.2 
                 globlastp 
               
               
                 960 
                 BLFS35 
                   Bradyrhizohium  sp. 
                 2810 
                 2477 
                 70.2 
                 globlastp 
               
               
                 1429 
                 BLFS109 
                   Pantoea  sp. 
                 3255 
                 2545 
                 89.2 
                 globlastp 
               
               
                 1430 
                 BLFS109 
                   Pantoea  sp. 
                 3256 
                 2545 
                 76.2 
                 globlastp 
               
               
                 1320 
                 BLFS101 
                 Environmental sample 
                 3155 
                 2537 
                 95.2 
                 globlastp 
               
               
                 1321 
                 BLFS101 
                   Pantoea  sp. 
                 3156 
                 2537 
                 94 
                 globlastp 
               
               
                 1322 
                 BLFS101 
                   Pantoea  sp. 
                 3157 
                 2537 
                 87.3 
                 globlastp 
               
               
                 1323 
                 BLFS101 
                 Environmental sample 
                 3158 
                 2537 
                 86.1 
                 globlastp 
               
               
                 1324 
                 BLFS101 
                 Environmental sample 
                 3159 
                 2537 
                 85.3 
                 globlastp 
               
               
                 1325 
                 BLFS101 
                   Pantoea  sp. 
                 3160 
                 2537 
                 84.1 
                 globlastp 
               
               
                 1326 
                 BLFS101 
                   Erwinia  sp. 
                 3161 
                 2537 
                 79.3 
                 globlastp 
               
               
                 1327 
                 BLFS101 
                   Serratia  sp. 
                 3162 
                 2537 
                 78.1 
                 globlastp 
               
               
                 1328 
                 BLFS101 
                   Erwinia  sp. 
                 3163 
                 2537 
                 77.3 
                 globlastp 
               
               
                 1329 
                 BLFS101 
                 Environmental sample 
                 3164 
                 2537 
                 76.1 
                 globlastp 
               
               
                 1330 
                 BLFS101 
                   Klebsiella  sp. 
                 3165 
                 2537 
                 75 
                 globlastp 
               
               
                 1331 
                 BLFS101 
                   Yersinia  sp. 
                 3166 
                 2537 
                 74.1 
                 globlastp 
               
               
                 1332 
                 BLFS101 
                 Environmental sample 
                 3167 
                 2537 
                 73 
                 globlastp 
               
               
                 1333 
                 BLFS101 
                   Rahnella  sp. 
                 3168 
                 2537 
                 72.1 
                 globlastp 
               
               
                 1334 
                 BLFS101 
                   Enterobacter  sp. 
                 3169 
                 2537 
                 71 
                 globlastp 
               
               
                 1335 
                 BLFS101 
                   Xenorhabdus  sp. 
                 3170 
                 2537 
                 70.1 
                 globlastp 
               
               
                 1607 
                 BLFS123 
                   Pseudomonas  sp. 
                 3431 
                 2558 
                 96.2 
                 globlastp 
               
               
                 1608 
                 BLFS123 
                   Pseudomonas  sp. 
                 3432 
                 2558 
                 95.9 
                 globlastp 
               
               
                 1609 
                 BLFS123 
                   Pseudomonas  sp. 
                 3433 
                 2558 
                 79.8 
                 globlastp 
               
               
                 1610 
                 BLFS123 
                   Pseudomonas  sp. 
                 3434 
                 2558 
                 79.5 
                 globlastp 
               
               
                 974 
                 BLFS41 
                   Burkholderia  sp. 
                 2822 
                 2482 
                 99.2 
                 globlastp 
               
               
                 975 
                 BLFS41 
                   Burkholderia  sp. 
                 2823 
                 2482 
                 98.9 
                 globlastp 
               
               
                 976 
                 BLFS41 
                   Burkholderia  sp. 
                 2824 
                 2482 
                 76.3 
                 globlastp 
               
               
                 977 
                 BLFS41 
                 Environmental sample 
                 2825 
                 2482 
                 75.1 
                 globlastp 
               
               
                 978 
                 BLFS41 
                   Burkholderia  sp. 
                 2826 
                 2482 
                 74 
                 globlastp 
               
               
                 979 
                 BLFS41 
                   Burkholderia  sp. 
                 2827 
                 2482 
                 73.1 
                 globlastp 
               
               
                 980 
                 BLFS41 
                   Burkholderia  sp. 
                 2828 
                 2482 
                 72.9 
                 globlastp 
               
               
                 981 
                 BLFS41 
                   Burkholderia  sp. 
                 2829 
                 2482 
                 71.3 
                 globlastp 
               
               
                 1064 
                 BLFS67 
                   Janthinobacterium  sp. 
                 2906 
                 2506 
                 98.6 
                 globlastp 
               
               
                 1065 
                 BLFS67 
                   Janthinobacterium  sp. 
                 2907 
                 2506 
                 93.8 
                 globlastp 
               
               
                 1066 
                 BLFS67 
                   Janthinobacterium  sp. 
                 2908 
                 2506 
                 92.5 
                 globlastp 
               
               
                 1276 
                 BLFS98 
                   Pantoea  sp. 
                 3111 
                 2534 
                 96.3 
                 globlastp 
               
               
                 1277 
                 BLFS98 
                 Environmental sample 
                 3112 
                 2534 
                 95.3 
                 globlastp 
               
               
                 1278 
                 BLFS98 
                   Pantoea  sp. 
                 3113 
                 2534 
                 93 
                 globlastp 
               
               
                 1279 
                 BLFS98 
                 Environmental sample 
                 3114 
                 2534 
                 91.4 
                 globlastp 
               
               
                 1280 
                 BLFS98 
                 Environmental sample 
                 3115 
                 2534 
                 89.8 
                 globlastp 
               
               
                 1281 
                 BLFS98 
                   Pantoea  sp. 
                 3116 
                 2534 
                 83.3 
                 globlastp 
               
               
                 1282 
                 BLFS98 
                 Environmental sample 
                 3117 
                 2534 
                 82.9 
                 globlastp 
               
               
                 1283 
                 BLFS98 
                   Pantoea  sp. 
                 3118 
                 2534 
                 81.5 
                 globlastp 
               
               
                 1284 
                 BLFS98 
                 Environmental sample 
                 3119 
                 2534 
                 79.4 
                 globlastp 
               
               
                 1285 
                 BLFS98 
                   Pantoea  sp. 
                 3120 
                 2534 
                 77.2 
                 globlastp 
               
               
                 1286 
                 BLFS98 
                   Pantoea  sp. 
                 3121 
                 2534 
                 76.1 
                 globlastp 
               
               
                 1287 
                 BLFS98 
                 Environmental sample 
                 3122 
                 2534 
                 75.3 
                 globlastp 
               
               
                 1288 
                 BLFS98 
                   Pantoea  sp. 
                 3123 
                 2534 
                 74 
                 globlastp 
               
               
                 1289 
                 BLFS98 
                 Environmental sample 
                 3124 
                 2534 
                 72.6 
                 globlastp 
               
               
                 1290 
                 BLFS98 
                   Erwinia  sp. 
                 3125 
                 2534 
                 71.6 
                 globlastp 
               
               
                 1291 
                 BLFS98 
                 Environmental sample 
                 3126 
                 2534 
                 70.2 
                 globlastp 
               
               
                 898 
                 BLFS26 
                   Bacillus  sp. 
                 2748 
                 2469 
                 99 
                 globlastp 
               
               
                 899 
                 BLFS26 
                   Bacillus  sp. 
                 2749 
                 2469 
                 98.4 
                 globlastp 
               
               
                 900 
                 BLFS26 
                   Bacillus  sp. 
                 2750 
                 2469 
                 97.9 
                 globlastp 
               
               
                 901 
                 BLFS26 
                   Bacillus  sp. 
                 2751 
                 2469 
                 96.3 
                 globlastp 
               
               
                 902 
                 BLFS26 
                   Bacillus  sp. 
                 2752 
                 2469 
                 72.3 
                 globlastp 
               
               
                 1838 
                 BLFS141 
                   Salinibacillus  sp. 
                 3661 
                 2576 
                 98.1 
                 globlastp 
               
               
                 1839 
                 BLFS141 
                   Bacillus  sp. 
                 3662 
                 2576 
                 97.5 
                 globlastp 
               
               
                 1840 
                 BLFS141 
                   Bacillus  sp. 
                 3663 
                 2576 
                 95.9 
                 globlastp 
               
               
                 1841 
                 BLFS141 
                   Bacillus  sp. 
                 3664 
                 2576 
                 90.1 
                 globlastp 
               
               
                 1842 
                 BLFS141 
                   Bacillus  sp. 
                 3665 
                 2576 
                 89 
                 globlastp 
               
               
                 1843 
                 BLFS141 
                   Bacillus  sp. 
                 3666 
                 2576 
                 88.1 
                 globlastp 
               
               
                 1844 
                 BLFS141 
                   Bacillus  sp. 
                 3667 
                 2576 
                 87.2 
                 globlastp 
               
               
                 1845 
                 BLFS141 
                   Bacillus  sp. 
                 3668 
                 2576 
                 86.6 
                 globlastp 
               
               
                 1846 
                 BLFS141 
                   Bacillus  sp. 
                 3669 
                 2576 
                 86.4 
                 globlastp 
               
               
                 1847 
                 BLFS141 
                   Bacillus  sp. 
                 3670 
                 2576 
                 83.7 
                 globlastp 
               
               
                 1848 
                 BLFS141 
                   Bacillus  sp. 
                 3671 
                 2576 
                 82.6 
                 globlastp 
               
               
                 1849 
                 BLFS141 
                   Bacillus  sp. 
                 3672 
                 2576 
                 77.1 
                 globlastp 
               
               
                 1850 
                 BLFS141 
                   Bacillus  sp. 
                 3673 
                 2576 
                 76 
                 globlastp 
               
               
                 1851 
                 BLFS141 
                   Bacillus  sp. 
                 3674 
                 2576 
                 75.6 
                 globlastp 
               
               
                 1852 
                 BLFS141 
                   Bacillus  sp. 
                 3675 
                 2576 
                 74 
                 globlastp 
               
               
                 1853 
                 BLFS141 
                   Bacillus  sp. 
                 3676 
                 2576 
                 73.2 
                 globlastp 
               
               
                 1854 
                 BLFS141 
                   Bacillus  sp. 
                 3677 
                 2576 
                 72.4 
                 globlastp 
               
               
                 1855 
                 BLFS141 
                   Bacillus  sp. 
                 3678 
                 2576 
                 71.5 
                 globlastp 
               
               
                 1856 
                 BLFS141 
                   Paenibacillus  sp. 
                 3679 
                 2576 
                 70.4 
                 globlastp 
               
               
                 1198 
                 BLFS88 
                   Paenibacillus  sp. 
                 3035 
                 2524 
                 99.2 
                 globlastp 
               
               
                 1199 
                 BLFS88 
                   Paenibacillus  sp. 
                 3036 
                 2524 
                 96.9 
                 globlastp 
               
               
                 1200 
                 BLFS88 
                   Paenibacillus  sp. 
                 3037 
                 2524 
                 95.3 
                 globlastp 
               
               
                 1201 
                 BLFS88 
                   Paenibacillus  sp. 
                 3038 
                 2524 
                 95 
                 globlastp 
               
               
                 1202 
                 BLFS88 
                   Paenibacillus  sp. 
                 3039 
                 2524 
                 94.4 
                 globlastp 
               
               
                 1203 
                 BLFS88 
                   Paenibacillus  sp. 
                 3040 
                 2524 
                 93.6 
                 globlastp 
               
               
                 1204 
                 BLFS88 
                   Paenibacillus  sp. 
                 3041 
                 2524 
                 92.5 
                 globlastp 
               
               
                 1205 
                 BLFS88 
                   Paenibacillus  sp. 
                 3042 
                 2524 
                 88.3 
                 globlastp 
               
               
                 1206 
                 BLFS88 
                   Paenibacillus  sp. 
                 3043 
                 2524 
                 72.9 
                 globlastp 
               
               
                 1010 
                 BLFS53 
                   Dickeya  sp. 
                 2856 
                 2494 
                 99 
                 globlastp 
               
               
                 878 
                 BLFS22 
                   Bacillus  sp. 
                 2728 
                 2465 
                 99.3 
                 globlastp 
               
               
                 879 
                 BLFS22 
                   Bacillus  sp. 
                 2729 
                 2465 
                 97.8 
                 globlastp 
               
               
                 880 
                 BLFS22 
                   Bacillus  sp. 
                 2730 
                 2465 
                 97.1 
                 globlastp 
               
               
                 881 
                 BLFS22 
                   Bacillus  sp. 
                 2731 
                 2465 
                 96.4 
                 globlastp 
               
               
                 882 
                 BLFS22 
                   Bacillus  sp. 
                 2732 
                 2465 
                 95.6 
                 globlastp 
               
               
                 883 
                 BLFS22 
                   Bacillus  sp. 
                 2733 
                 2465 
                 94.9 
                 globlastp 
               
               
                 819 
                 BLFS12 
                   Arthrobacter  sp. 
                 2671 
                 2455 
                 98.9 
                 globlastp 
               
               
                 820 
                 BLFS12 
                   Arthrobacter  sp. 
                 2672 
                 2455 
                 92.3 
                 globlastp 
               
               
                 1960 
                 BLFS149 
                   Serratia  sp. 
                 3782 
                 2584 
                 98.8 
                 globlastp 
               
               
                 1961 
                 BLFS149 
                   Serratia  sp. 
                 3783 
                 2584 
                 97 
                 globlastp 
               
               
                 1962 
                 BLFS149 
                   Serratia  sp. 
                 3784 
                 2584 
                 93.8 
                 globlastp 
               
               
                 1963 
                 BLFS149 
                   Serratia  sp. 
                 3785 
                 2584 
                 91.7 
                 globlastp 
               
               
                 1964 
                 BLFS149 
                   Serratia  sp. 
                 3786 
                 2584 
                 84 
                 globlastp 
               
               
                 1965 
                 BLFS149 
                   Serratia  sp. 
                 3787 
                 2584 
                 83.8 
                 globlastp 
               
               
                 1966 
                 BLFS149 
                   Serratia  sp. 
                 3788 
                 2584 
                 82 
                 globlastp 
               
               
                 1967 
                 BLFS149 
                   Serratia  sp. 
                 3789 
                 2584 
                 81.3 
                 globlastp 
               
               
                 1968 
                 BLFS149 
                   Serratia  sp. 
                 3790 
                 2584 
                 78 
                 globlastp 
               
               
                 1969 
                 BLFS149 
                   Serratia  sp. 
                 3791 
                 2584 
                 77.8 
                 globlastp 
               
               
                 1970 
                 BLFS149 
                   Yersinia  sp. 
                 3792 
                 2584 
                 76 
                 globlastp 
               
               
                 1971 
                 BLFS149 
                   Yersinia  sp. 
                 3793 
                 2584 
                 75 
                 globlastp 
               
               
                 1972 
                 BLFS149 
                   Yersinia  sp. 
                 3794 
                 2584 
                 74 
                 globlastp 
               
               
                 1973 
                 BLFS149 
                   Yersinia  sp. 
                 3795 
                 2584 
                 73.1 
                 globlastp 
               
               
                 1974 
                 BLFS149 
                   Edwardsiella  sp. 
                 3796 
                 2584 
                 72 
                 globlastp 
               
               
                 1975 
                 BLFS149 
                 Environmental sample 
                 3797 
                 2584 
                 71.2 
                 globlastp 
               
               
                 1984 
                 BLFS152 
                   Streptomyces  sp. 
                 3801 
                 2587 
                 82.9 
                 globlastp 
               
               
                 1985 
                 BLFS152 
                   Streptomyces  sp. 
                 3802 
                 2587 
                 78.9 
                 globlastp 
               
               
                 1995 
                 BLFS154 
                   Streptomyces  sp. 
                 3809 
                 2589 
                 98.2 
                 globlastp 
               
               
                 1996 
                 BLFS154 
                   Streptomyces  sp. 
                 3810 
                 2589 
                 72.2 
                 globlastp 
               
               
                 1432 
                 BLFS111 
                   Chromobacterium  sp. 
                 3258 
                 2547 
                 97 
                 globlastp 
               
               
                 1433 
                 BLFS111 
                   Chromobacterium  sp. 
                 3259 
                 2547 
                 89.9 
                 globlastp 
               
               
                 1434 
                 BLFS111 
                   Chromobacterium  sp. 
                 3260 
                 2547 
                 84.4 
                 globlastp 
               
               
                 1435 
                 BLFS111 
                   Chromobacterium  sp. 
                 3261 
                 2547 
                 83.4 
                 globlastp 
               
               
                 1436 
                 BLFS111 
                   Paludibacterium  sp. 
                 3262 
                 2547 
                 71.4 
                 globlastp 
               
               
                 791 
                 BLFS2 
                   Alkanindiges  sp. 
                 2643 
                 2445 
                 91.7 
                 globlastp 
               
               
                 1644 
                 BLFS126 
                   Pseudomonas  sp. 
                 3468 
                 2561 
                 98.3 
                 globlastp 
               
               
                 1645 
                 BLFS126 
                   Pseudomonas  sp. 
                 3469 
                 2561 
                 96.7 
                 globlastp 
               
               
                 1646 
                 BLFS126 
                   Pseudomonas  sp. 
                 3470 
                 2561 
                 92.6 
                 globlastp 
               
               
                 1647 
                 BLFS126 
                   Pseudomonas  sp. 
                 3471 
                 2561 
                 91.7 
                 globlastp 
               
               
                 1648 
                 BLFS126 
                   Pseudomonas  sp. 
                 3472 
                 2561 
                 90.9 
                 globlastp 
               
               
                 1649 
                 BLFS126 
                   Pseudomonas  sp. 
                 3473 
                 2561 
                 80.3 
                 globlastp 
               
               
                 1650 
                 BLFS126 
                   Pseudomonas  sp. 
                 3474 
                 2561 
                 75.6 
                 globlastp 
               
               
                 1651 
                 BLFS126 
                   Pseudomonas  sp. 
                 3475 
                 2561 
                 73.2 
                 globlastp 
               
               
                 2286 
                 ZLFS34 
                   Serratia  sp. 
                 4100 
                 2629 
                 99 
                 globlastp 
               
               
                 2287 
                 ZLFS34 
                   Serratia  sp. 
                 4101 
                 2629 
                 98.4 
                 globlastp 
               
               
                 2288 
                 ZLFS34 
                 Multievos46 sp. 
                 4102 
                 2629 
                 97 
                 globlastp 
               
               
                 2289 
                 ZLFS34 
                   Serratia  sp. 
                 4103 
                 2629 
                 96 
                 globlastp 
               
               
                 2290 
                 ZLFS34 
                   Serratia  sp. 
                 4104 
                 2629 
                 95.9 
                 globlastp 
               
               
                 2291 
                 ZLFS34 
                   Serratia  sp. 
                 4105 
                 2629 
                 93 
                 globlastp 
               
               
                 2292 
                 ZLFS34 
                   Serratia  sp. 
                 4106 
                 2629 
                 92.1 
                 globlastp 
               
               
                 2293 
                 ZLFS34 
                   Serratia  sp. 
                 4107 
                 2629 
                 91.4 
                 globlastp 
               
               
                 2294 
                 ZLFS34 
                   Serratia  sp. 
                 4108 
                 2629 
                 88.1 
                 globlastp 
               
               
                 2295 
                 ZLFS34 
                   Serratia  sp. 
                 4109 
                 2629 
                 87 
                 globlastp 
               
               
                 2296 
                 ZLFS34 
                   Serratia  sp. 
                 4110 
                 2629 
                 84.3 
                 globlastp 
               
               
                 2297 
                 ZLFS34 
                   Xenorhabdus  sp. 
                 4111 
                 2629 
                 83.5 
                 globlastp 
               
               
                 2298 
                 ZLFS34 
                   Xenorhabdus  sp. 
                 4112 
                 2629 
                 82 
                 globlastp 
               
               
                 2299 
                 ZLFS34 
                   Xenorhabdus  sp. 
                 4113 
                 2629 
                 80.6 
                 globlastp 
               
               
                 2300 
                 ZLFS34 
                   Yersinia  sp. 
                 4114 
                 2629 
                 78.8 
                 globlastp 
               
               
                 2301 
                 ZLFS34 
                   Yersinia  sp. 
                 4115 
                 2629 
                 75 
                 globlastp 
               
               
                 2302 
                 ZLFS34 
                 Environmental sample 
                 4116 
                 2629 
                 74.5 
                 globlastp 
               
               
                 2303 
                 ZLFS34 
                   Rahnella  sp. 
                 4117 
                 2629 
                 73.1 
                 globlastp 
               
               
                 2304 
                 ZLFS34 
                   Ewingella  sp. 
                 4118 
                 2629 
                 71.5 
                 globlastp 
               
               
                 2305 
                 ZLFS34 
                   Rhodanobacter  sp. 
                 4119 
                 2629 
                 70.1 
                 globlastp 
               
               
                 1907 
                 BLFS146 
                   Bacillus  sp. 
                 3730 
                 2581 
                 98.8 
                 globlastp 
               
               
                 1908 
                 BLFS146 
                   Bacillus  sp. 
                 3731 
                 2581 
                 97.2 
                 globlastp 
               
               
                 1909 
                 BLFS146 
                   Bacillus  sp. 
                 3732 
                 2581 
                 96 
                 globlastp 
               
               
                 1910 
                 BLFS146 
                   Bacillus  sp. 
                 3733 
                 2581 
                 95.4 
                 globlastp 
               
               
                 1911 
                 BLFS146 
                   Bacillus  sp. 
                 3734 
                 2581 
                 94 
                 globlastp 
               
               
                 1912 
                 BLFS146 
                   Bacillus  sp. 
                 3735 
                 2581 
                 93.4 
                 globlastp 
               
               
                 1913 
                 BLFS146 
                   Bacillus  sp. 
                 3736 
                 2581 
                 92.9 
                 globlastp 
               
               
                 1914 
                 BLFS146 
                   Bacillus  sp. 
                 3737 
                 2581 
                 91.7 
                 globlastp 
               
               
                 1915 
                 BLFS146 
                   Bacillus  sp. 
                 3738 
                 2581 
                 89.1 
                 globlastp 
               
               
                 1916 
                 BLFS146 
                   Bacillus  sp. 
                 3739 
                 2581 
                 85 
                 globlastp 
               
               
                 1917 
                 BLFS146 
                   Bacillus  sp. 
                 3740 
                 2581 
                 84.7 
                 globlastp 
               
               
                 1918 
                 BLFS146 
                   Bacillus  sp. 
                 3741 
                 2581 
                 84 
                 globlastp 
               
               
                 1919 
                 BLFS146 
                   Bacillus  sp. 
                 3742 
                 2581 
                 81.2 
                 globlastp 
               
               
                 1920 
                 BLFS146 
                   Bacillus  sp. 
                 3743 
                 2581 
                 78.2 
                 globlastp 
               
               
                 1921 
                 BLFS146 
                   Bacillus  sp. 
                 3744 
                 2581 
                 77.9 
                 globlastp 
               
               
                 1922 
                 BLFS146 
                   Bacillus  sp. 
                 3745 
                 2581 
                 76.4 
                 globlastp 
               
               
                 1923 
                 BLFS146 
                   Bacillus  sp. 
                 3746 
                 2581 
                 74 
                 globlastp 
               
               
                 1924 
                 BLFS146 
                   Bacillus  sp. 
                 3747 
                 2581 
                 73.1 
                 globlastp 
               
               
                 1925 
                 BLFS146 
                   Bacillus  sp. 
                 3748 
                 2581 
                 71.1 
                 globlastp 
               
               
                 1926 
                 BLFS146 
                   Bacillus  sp. 
                 3749 
                 2581 
                 70.8 
                 globlastp 
               
               
                 1749 
                 BLFS133 
                   Pseudomonas  sp. 
                 3573 
                 2568 
                 93.2 
                 globlastp 
               
               
                 1750 
                 BLFS133 
                   Pseudomonas  sp. 
                 3574 
                 2568 
                 91.8 
                 globlastp 
               
               
                 1751 
                 BLFS133 
                   Pseudomonas  sp. 
                 3575 
                 2568 
                 79.5 
                 globlastp 
               
               
                 1752 
                 BLFS133 
                   Pseudomonas  sp. 
                 3576 
                 2568 
                 78.1 
                 globlastp 
               
               
                 893 
                 BLFS25 
                   Bacillus  sp. 
                 2743 
                 2468 
                 99.2 
                 globlastp 
               
               
                 894 
                 BLFS25 
                   Bacillus  sp. 
                 2744 
                 2468 
                 98.8 
                 globlastp 
               
               
                 895 
                 BLFS25 
                   Bacillus  sp. 
                 2745 
                 2468 
                 98.4 
                 globlastp 
               
               
                 896 
                 BLFS25 
                   Bacillus  sp. 
                 2746 
                 2468 
                 98 
                 globlastp 
               
               
                 897 
                 BLFS25 
                   Bacillus  sp. 
                 2747 
                 2468 
                 70.8 
                 globlastp 
               
               
                 2070 
                 ZLFS5 
                   Burkholderia  sp. 
                 3884 
                 2602 
                 98.4 
                 globlastp 
               
               
                 2071 
                 ZLFS5 
                   Burkholderia  sp. 
                 3885 
                 2602 
                 97.7 
                 globlastp 
               
               
                 2072 
                 ZLFS5 
                   Burkholderia  sp. 
                 3886 
                 2602 
                 96.1 
                 globlastp 
               
               
                 2073 
                 ZLFS5 
                   Burkholderia  sp. 
                 3887 
                 2602 
                 88.3 
                 globlastp 
               
               
                 2074 
                 ZLFS5 
                   Burkholderia  sp. 
                 3888 
                 2602 
                 75 
                 globlastp 
               
               
                 1981 
                 BLFS151 
                   Stenotrophomonas  sp. 
                 3798 
                 2586 
                 78.1 
                 globlastp 
               
               
                 1982 
                 BLFS151 
                   Stenotrophomonas  sp. 
                 3799 
                 2586 
                 75.7 
                 globlastp 
               
               
                 1983 
                 BLFS151 
                   Stenotrophomonas  sp. 
                 3800 
                 2586 
                 73.5 
                 globlastp 
               
               
                 2000 
                 BLFS159 
                   Xanthomonas  sp. 
                 3814 
                 2594 
                 79.9 
                 globlastp 
               
               
                 2001 
                 BLFS159 
                   Xanthomonas  sp. 
                 3815 
                 2594 
                 76.8 
                 globlastp 
               
               
                 2089 
                 ZLFS13 
                   Collimonas  sp. 
                 3903 
                 2610 
                 96.7 
                 globlastp 
               
               
                 2090 
                 ZLFS13 
                   Collimonas  sp. 
                 3904 
                 2610 
                 80.3 
                 globlastp 
               
               
                 2032 
                 ZLFS1 
                   Actinomyces  sp. 
                 3846 
                 2599 
                 73.5 
                 globlastp 
               
               
                 1005 
                 BLFS50 
                   Collimonas  sp. 
                 2853 
                 2491 
                 85.1 
                 globlastp 
               
               
                 2091 
                 ZLFS14 
                   Collimonas  sp. 
                 3905 
                 2611 
                 75 
                 globlastp 
               
               
                 2083 
                 ZLFS9 
                   Collimonas  sp. 
                 3897 
                 2606 
                 98.6 
                 globlastp 
               
               
                   
               
               
                 “Polyn.” = polynucleotide; “Polyp.” = polypeptide; “Algor.” = algorithm (used for sequence alignment and determination of percent homology); “Hom.”—homology; “iden.”—identity; “glob.”—global. Environmental sample = unidentified organisms obtained from the sample. 
               
            
           
         
       
     
     The output of the functional genomics approach described herein is a set of genes highly predicted to improve plant resistance to fungal infection. 
     Example 13: Identification of Domains Comprised within Polypeptides Encoded by the Identified Genes 
     Polypeptide domains were identified as described in Example 4 hereinabove. The polypeptides of some embodiments of the invention, the expression of which in a may result in fungal control by invoking antifungal effects, can be characterized by specific amino acid domains. According to certain embodiments of the invention, particular domains are conserved within a family of polypeptides as described in Table 17 hereinbelow. Without wishing to be bound by specific theory or mechanism of action, the conserved domain may indicate common function of the polypeptides comprising same. The domains are presented by an arbitrary identifier (*ID). Table 18 provides the details of each domain according to the InterPro Entry. 
     Table 17 summarizes the domains in each of the “core” polypeptides (e.g., the polypeptides from Table 15) identified by the present inventors as being capable of conferring resistant to fungi and/or Oomycetes infection when its expression is modified in a plant (e.g. enhanced or downregulated), wherein each of the listed domains is conserved in the representative homologous polypeptides identified by the present inventors exhibiting at least 70% global identity to the “core” polypeptides (as detailed in Table 16 in Example 12 above). As explained above, each domain received an arbitrary ID number wherein description of these arbitrary domain IDs according to the InterPro database is provided in Table 18 below. In addition, the start and end position of each of the domains with respect to the amino acid sequence of the “core” polypeptide was identified, as well as the E-values for each of the conserved domains as indicated by the domain tool used for analyzing these sequences, as part of interproscan programs, e.g., SMART, prosite scans patterns and profiles (not shown). For example, in the case of the Prosite search, the Prosite profiles report normalized scores instead of E-values, which are defined as the base 10 logarithm of the size (in residues) of the database in which one false positive match is expected to occur by chance. The normalized score is independent of the size of the databases searched. The so-called bit scores reported by other database-search programs have a distinct meaning but are also independent of the size of the database searched 
     For example, for SEQ ID NO:9060, the domain ID “1” appears at amino acid positions 37 through 124. For some domains the e-value was not specified. In these cases, the presence of the domain was verified by ScanRegExp, which is able to verify PROSITE matches using corresponding statistically-significant CONFIRM patterns. The CONFIRM patterns were generated based on the true positive SWISS-PROT PROSITE matches using eMOTIF software with a stringency of 10e-9 P-value. Further details can be found in hypertext transfer protocol://computing.bio.cam.ac.uk/local/doc/iprscan.html. 
     
       
         
           
               
             
               
                 TABLE 17 
               
             
            
               
                   
               
               
                 Core and homologous polypeptides comprising the same domains 
               
            
           
           
               
               
               
               
            
               
                 Core 
                   
                   
                   
               
               
                 Polypeptide 
                 Gene 
                   
                 Homologous Polypeptides 
               
               
                 (SEQ ID 
                 Name 
                 Characteristic Domains 
                 Comprising the Domains 
               
               
                 NO) 
                 (Core) 
                 (Domain identifier) 
                 (SEQ ID NOs) 
               
               
                   
               
               
                 2444 
                 BLFS1 
                 1; 2; 3 in core and homologs 
                 2642 
               
               
                 2445 
                 BLFS2 
                 4; 5; 6 in core and homologs 
                 2643 
               
               
                 2446 
                 BLFS3 
                 7; 8; 9; 10; 11; 12 in core 
               
               
                 2447 
                 BLFS4 
                 6 in core 
               
               
                 2448 
                 BLFS5 
                 13; 14 in core and homologs 
                 2644; 2645; 2646; 2647; 2648; 2649; 2 
               
               
                   
                   
                   
                 650; 2651 
               
               
                 2449 
                 BLFS6 
                 2; 3 in core 
               
               
                 2450 
                 BLFS7 
                 15; 16 in core 
               
               
                 2451 
                 BLFS8 
                 1; 2; 3 in core and homologs 
                 2652; 2653; 2654 
               
               
                 2452 
                 BLFS9 
                 2; 1; 3 in core and homologs 
                 2655; 2656; 2657; 2658; 2659; 2660 
               
               
                 2453 
                 BLFS10 
                 1; 2; 3 in core and homologs 
                 2452; 2655; 2656; 2661; 2662; 2663 
               
               
                 2454 
                 BLFS11 
                 no domains in core 
               
               
                 2455 
                 BLFS12 
                 no domains in core 
               
               
                 2456 
                 BLFS13 
                 no domains in core 
               
               
                 2457 
                 BLFS14 
                 17 in core and homologs 
                 2675; 2676 
               
               
                 2458 
                 BLFS15 
                 18; 19; 20 in core and homologs 
                 2677; 2678; 2679; 2680; 2681; 2682; 2 
               
               
                   
                   
                   
                 683; 2684; 2685 
               
               
                 2459 
                 BLFS16 
                 21; 22; 23; 24 in core and homologs 
                 2686; 2687; 2688; 2689; 2690 
               
               
                 2460 
                 BLFS17 
                 25; 26 in core and homologs 
                 2691; 2692; 2693; 2694; 2695; 2696 
               
               
                 2461 
                 BLFS18 
                 27; 28; 29 in core and homologs 
                 2697; 2698; 2699; 2700; 2701; 2702; 2 
               
               
                   
                   
                   
                 703; 2704; 2705; 2706; 2707; 2708; 27 
               
               
                   
                   
                   
                 09; 2710 
               
               
                 2462 
                 BLFS19 
                 8; 30 in core and homologs 
                 2711; 2712; 2713; 2714; 2715; 2716; 2 
               
               
                   
                   
                   
                 717 
               
               
                 2463 
                 BLFS20 
                 31; 32; 33 in core and homologs 
                 2718; 2719; 2720; 2721 
               
               
                 2464 
                 BLFS21 
                 34; 35; 36; 37; 38 in core and 
                 2722; 2723; 2724; 2725; 2726; 2727 
               
               
                   
                   
                 homologs 
               
               
                 2465 
                 BLFS22 
                 no domains in core 
               
               
                 2466 
                 BLFS23 
                 39; 31; 40; 41; 42; 32; 24 in core and 
                 2734; 2735; 2736; 2737; 2738; 2739; 2 
               
               
                   
                   
                 homologs 
                 740; 2741; 2742 
               
               
                 2467 
                 BLFS24 
                 no domains in core 
               
               
                 2468 
                 BLFS25 
                 43; 44 in core and homologs 
                 2743; 2744; 2745; 2746; 2747 
               
               
                 2469 
                 BLFS26 
                 31; 45; 32 in core and homologs 
                 2748; 2749; 2750; 2751; 2752 
               
               
                 2470 
                 BLFS27 
                 46; 47; 48 in core and homologs 
                 2753; 2754; 2755; 2756; 2757 
               
               
                 2471 
                 BLFS29 
                 8; 49; 30 in core and homologs 
                 2758 
               
               
                 2472 
                 BLFS30 
                 6 in core 
               
               
                 2473 
                 BLFS31 
                 50 in core and homologs 
                 2759; 2760; 2761; 2762; 2763; 2764; 2 
               
               
                   
                   
                   
                 765; 2766; 2767; 2768; 2769 
               
               
                 2474 
                 BLFS32 
                 52 in core and homologs 
                 2770; 2771; 2772; 2773; 2774; 2775; 2 
               
               
                   
                   
                   
                 776; 2777; 2778; 2779; 2780; 2781; 27 
               
               
                   
                   
                   
                 82; 2783; 2784; 2785; 2786 
               
               
                 2475 
                 BLFS33 
                 53; 35; 54 in core and homologs 
                 2787; 2788; 2789; 2790; 2791; 2792; 2 
               
               
                   
                   
                   
                 793; 2794; 2795; 2796; 2797; 2798 
               
               
                 2476 
                 BLFS34 
                 31; 32; 33 in core and homologs 
                 2799; 2800; 2801 
               
               
                 2477 
                 BLFS35 
                 55 in core and homologs 
                 2802; 2803; 2804; 2805; 2806; 2807; 2 
               
               
                   
                   
                   
                 808; 2809; 2810 
               
               
                 2478 
                 BLFS36 
                 no domains in core 
               
               
                 2479 
                 BLFS37 
                 1; 56; 57 in core and homologs 
                 2816; 2817 
               
               
                 2480 
                 BLFS38 
                 58; 59 in core and homologs 
                 2818 
               
               
                 2481 
                 BLFS39 
                 6 in core and homologs 
                 2819; 2820; 2821 
               
               
                 2482 
                 BLFS41 
                 60; 61; 62; 63; 24; 64; 65; 66; 67 in core 
                 2822; 2823; 2824; 2825; 2826; 2827; 2 
               
               
                   
                   
                 and homologs 
                 828; 2829 
               
               
                 2483 
                 BLFS42 
                 60; 68 in core and homologs 
                 2830; 2831 
               
               
                 2484 
                 BLFS43 
                 51 in core and homologs 
                 2832; 2833; 2834; 2835 
               
               
                 2485 
                 BLFS44 
                 69; 70; 71; 7; 10; 24 in core and 
                 2836 
               
               
                   
                   
                 homologs 
               
               
                 2486 
                 BLFS45 
                 73 in core and homologs 
                 2837; 2838; 2839; 2840; 2841; 2842; 2 
               
               
                   
                   
                   
                 843; 2844 
               
               
                 2487 
                 BLFS46 
                 31; 74; 32; 75 in core and homologs 
                 2845 
               
               
                 2488 
                 BLFS47 
                 1 in core and homologs 
                 2846; 2847; 2848; 2849 
               
               
                 2489 
                 BLFS48 
                 35; 52; 37 in core and homologs 
                 2850; 2851; 2852 
               
               
                 2490 
                 BLFS49 
                 7; 10 in core 
               
               
                 2491 
                 BLFS50 
                 60; 68 in core and homologs 
                 2853 
               
               
                 2492 
                 BLFS51 
                 76; 24 in core and homologs 
                 2854; 2855 
               
               
                 2493 
                 BLFS52 
                 7; 10 in core 
               
               
                 2494 
                 BLFS53 
                 no domains in core 
               
               
                 2495 
                 BLFS55 
                 1; 57 in core and homologs 
                 2857; 2858 
               
               
                 2496 
                 BLFS56 
                 77; 17; 79 in core and homologs 
                 2859; 2860; 2861; 2862; 2863; 2864 
               
               
                 2497 
                 BLFS57 
                 80; 81; 7; 10; 82 in core and 
                 2865; 2866; 2867 
               
               
                   
                   
                 homologs 
               
               
                 2498 
                 BLFS58 
                 1; 83; 57 in core and homologs 
                 2868; 2869 
               
               
                 2499 
                 BLFS59 
                 1; 57 in core 
               
               
                 2500 
                 BLFS60 
                 1 in core and homologs 
                 2870; 2871; 2872 
               
               
                 2501 
                 BLFS61 
                 84; 85; 86; 87; 88 in core and 
                 2873; 2874; 2875; 2876; 2877; 2878; 2 
               
               
                   
                   
                 homologs 
                 879; 2880 
               
               
                 2502 
                 BLFS62 
                 31; 74; 32; 75 in core and homologs 
                 2881; 2882; 2883; 2884; 2885; 2886; 2 
               
               
                   
                   
                   
                 887; 2888; 2889; 2890 
               
               
                 2503 
                 BLFS63 
                 89; 90; 31; 32 in core and homologs 
                 2891; 2892; 2893; 2894; 2895; 2896; 2 
               
               
                   
                   
                   
                 897; 2898; 2899; 2900; 2901; 2902; 29 
               
               
                   
                   
                   
                 03 
               
               
                 2504 
                 BLFS64 
                 8; 49; 30 in core and homologs 
                 2904 
               
               
                 2505 
                 BLFS65 
                 91 in core and homologs 
                 2905 
               
               
                 2506 
                 BLFS67 
                 1; 57 in core and homologs 
                 2906; 2907; 2908 
               
               
                 2507 
                 BLFS68 
                 92 in core and homologs 
                 2909; 2910; 2911; 2912 
               
               
                 2508 
                 BLFS69 
                 93; 94; 95; 96; 97 in core 
               
               
                 2509 
                 BLFS70 
                 98 in core 
               
               
                 2510 
                 BLFS72 
                 99; 100; 101; 102 in core and 
                 2913 
               
               
                   
                   
                 homologs 
               
               
                 2511 
                 BLFS73 
                 1 in core and homologs 
                 2914; 2915; 2916; 2917; 2918; 2919; 2 
               
               
                   
                   
                   
                 920; 2921; 2922; 2923; 2924; 2925; 29 
               
               
                   
                   
                   
                 26; 2927; 2928; 2929 
               
               
                 2512 
                 BLFS74 
                 34 in core and homologs 
                 2930; 2931; 2932; 2933; 2934; 2935; 2 
               
               
                   
                   
                   
                 936; 2937; 2938; 2939; 2940; 2941; 29 
               
               
                   
                   
                   
                 42; 2943 
               
               
                 2513 
                 BLFS75 
                 103; 104 in core and homologs 
                 2944; 2945; 2946; 2947; 2948; 2949; 2 
               
               
                   
                   
                   
                 950; 2951; 2952; 2953; 2954 
               
               
                 2514 
                 BLFS76 
                 no domains in core 
               
               
                 2515 
                 BLFS77 
                 17; 7; 78; 79; 10; 24; 76 in core and 
                 2963; 2964; 2965 
               
               
                   
                   
                 homologs 
               
               
                 2516 
                 BLFS79 
                 43; 44; 105 in core and homologs 
                 2966; 2967; 2968; 2969; 2970; 2971; 2 
               
               
                   
                   
                   
                 972; 2973; 2974; 2975 
               
               
                 2517 
                 BLFS80 
                 17; 78; 19 in core 
               
               
                 2518 
                 BLFS82 
                 no domains in core 
               
               
                 2519 
                 BLFS83 
                 106; 79; 107; 108; 17; 66; 78; 109 in 
                 2989; 2990; 2991; 2992; 2993; 2994; 2 
               
               
                   
                   
                 core and homologs 
                 995; 2996; 2997; 2998; 2999 
               
               
                 2520 
                 BLFS84 
                 86; 110 in core and homologs 
                 3000; 3001; 3002; 3003; 3004; 3005; 3 
               
               
                   
                   
                   
                 006 
               
               
                 2521 
                 BLFS85 
                 71; 24; 111 in core and homologs 
                 3007; 3008; 3009; 3010; 3011; 3012; 3 
               
               
                   
                   
                   
                 013; 3014; 3015; 3016; 3017 
               
               
                 2522 
                 BLFS86 
                 no domains in core 
               
               
                 2523 
                 BLFS87 
                 17; 60; 112; 76; 24; 113; 114 in core 
                 3024; 3025; 3026; 3027; 3028; 3029; 3 
               
               
                   
                   
                 and homologs 
                 030; 3031; 3032; 3033; 3034 
               
               
                 2524 
                 BLFS88 
                 no domains in core 
               
               
                 2525 
                 BLFS89 
                 24; 113; 76 in core and homologs 
                 3044; 3045; 3046; 3047; 3048; 3049; 3 
               
               
                   
                   
                   
                 050; 3051; 3052 
               
               
                 2526 
                 BLFS90 
                 115; 116 in core and homologs 
                 3053; 3054; 3055; 3056; 3057; 3058; 3 
               
               
                   
                   
                   
                 059; 3060; 3061 
               
               
                 2527 
                 BLFS91 
                 no domains in core 
               
               
                 2528 
                 BLFS92 
                 34; 35; 36; 37; 38 in core and 
                 3073; 3074; 3075; 3076; 3077; 3078; 3 
               
               
                   
                   
                 homologs 
                 079; 3080; 3081 
               
               
                 2529 
                 BLFS93 
                 117; 24 in core and homologs 
                 3082; 3083; 3084; 3085; 3086; 3087; 3 
               
               
                   
                   
                   
                 088; 3089; 3090; 3091; 3092 
               
               
                 2530 
                 BLFS94 
                 17; 118 in core and homologs 
                 3093; 3094; 3095; 3096; 3097; 3098; 3 
               
               
                   
                   
                   
                 099; 3100; 3101 
               
               
                 2531 
                 BLFS95 
                 no domains in core 
               
               
                 2532 
                 BLFS96 
                 7; 10; 6 in core 
               
               
                 2533 
                 BLFS97 
                 119 in core 
               
               
                 2534 
                 BLFS98 
                 34 in core and homologs 
                 3111; 3112; 3113; 3114; 3115; 3116; 3 
               
               
                   
                   
                   
                 117; 3118; 3119; 3120; 3121; 3122; 31 
               
               
                   
                   
                   
                 23; 3124; 3125; 3126 
               
               
                 2535 
                 BLFS99 
                 120; 121; 122 in core and homologs 
                 3127; 3128; 3129; 3130; 3131; 3132; 3 
               
               
                   
                   
                   
                 133; 3134; 3135; 3136; 3137; 3138 
               
               
                 2536 
                 BLFS100 
                 120; 121; 122 in core and homologs 
                 3139; 3140; 3141; 3142; 3143; 3144; 3 
               
               
                   
                   
                   
                 145; 3146; 3147; 3148; 3149; 315 0; 31 
               
               
                   
                   
                   
                 51; 3152; 3153; 3154 
               
               
                 2537 
                 BLFS101 
                 52 in core and homologs 
                 3155; 3156; 3157; 3158; 3159; 3160; 3 
               
               
                   
                   
                   
                 161; 3162; 3163; 3164; 3165; 3166; 31 
               
               
                   
                   
                   
                 67; 3168; 3169; 3170 
               
               
                 2538 
                 BLFS102 
                 no domains in core 
               
               
                 2539 
                 BLFS103 
                 no domains in core 
               
               
                 2540 
                 BLFS104 
                 no domains in core 
               
               
                 2541 
                 BLFS105 
                 123 in core and homologs 
                 3194; 3195; 3196; 3197; 3198; 3199; 3 
               
               
                   
                   
                   
                 200; 3201; 3202; 3203; 3204; 3205; 32 
               
               
                   
                   
                   
                 06 
               
               
                 2542 
                 BLFS106 
                 124 in core and homologs 
                 3207; 3208; 3209; 3210; 3211; 3212; 3 
               
               
                   
                   
                   
                 213; 3214; 3215; 3216; 3217; 3218; 32 
               
               
                   
                   
                   
                 19; 3220; 3221; 3222; 3223; 3224; 322 
               
               
                   
                   
                   
                 5; 3226; 3227 
               
               
                 2543 
                 BLFS107 
                 125 in core and homologs 
                 3228; 3229; 3230; 3231; 3232; 3233; 3 
               
               
                   
                   
                   
                 234; 3235; 3236; 3237; 3238; 3239; 32 
               
               
                   
                   
                   
                 40; 3241; 3242; 3243; 3244; 3245 
               
               
                 2544 
                 BLFS108 
                 126 in core and homologs 
                 3246; 3247; 3248; 3249; 3250; 3251; 3 
               
               
                   
                   
                   
                 252; 3253; 3254 
               
               
                 2545 
                 BLFS109 
                 127 in core and homologs 
                 3255; 3256 
               
               
                 2546 
                 BLFS110 
                 128 in core and homologs 
                 3257 
               
               
                 2547 
                 BLFS111 
                 129 in core and homologs 
                 3258; 3259; 3260; 3261; 3262 
               
               
                 2548 
                 BLFS112 
                 no domains in core 
               
               
                 2549 
                 BLFS113 
                 130; 131 in core and homologs 
                 3276; 3277; 3278; 3279; 3280; 3281; 3 
               
               
                   
                   
                   
                 282; 3283; 3284; 3285; 3286; 3287; 32 
               
               
                   
                   
                   
                 88; 3289; 3290; 3291; 3292; 3293; 329 
               
               
                   
                   
                   
                 4; 3295; 3296; 3297; 3298; 3299; 3300 
               
               
                 2550 
                 BLFS114 
                 132 in core and homologs 
                 3301; 3302; 3303; 3304; 3305; 3306; 3 
               
               
                   
                   
                   
                 307; 3308; 3309; 3310; 3311; 3312; 33 
               
               
                   
                   
                   
                 13; 3314; 3315; 3316; 3317; 3318; 331 
               
               
                   
                   
                   
                 9; 3320; 3321; 3322; 3323; 3324; 3325 
               
               
                 2551 
                 BLFS115 
                 34; 133; 35; 37 in core and homologs 
                 3326; 3327; 3328; 3329; 3330; 3331; 3 
               
               
                   
                   
                   
                 332; 3333; 3334; 3335; 3336; 3337; 33 
               
               
                   
                   
                   
                 38; 3339; 3340; 3341; 3342; 3343; 334 
               
               
                   
                   
                   
                 4; 3345; 3346; 3347; 3348; 3349; 3350; 
               
               
                   
                   
                   
                 3351; 3352; 3353; 3354 
               
               
                 2552 
                 BLFS116 
                 86; 110 in core and homologs 
                 3355; 3356; 3357; 3358; 3359; 3360; 3 
               
               
                   
                   
                   
                 361; 3362; 3363; 3364; 3365; 3366; 33 
               
               
                   
                   
                   
                 67; 3368; 3369; 3370; 3371; 3372; 337 
               
               
                   
                   
                   
                 3; 3374; 3375; 3376 
               
               
                 2553 
                 BLFS118 
                 31; 32 in core and homologs 
                 3377; 3378; 3379; 3380; 3381; 3382; 3 
               
               
                   
                   
                   
                 383; 3384; 3385; 3386; 3387; 3388; 33 
               
               
                   
                   
                   
                 89; 3390; 3391; 3392; 3393; 3394 
               
               
                 2554 
                 BLFS119 
                 no domains in core 
               
               
                 2555 
                 BLFS120 
                 134; 135 in core and homologs 
                 3399; 3400; 3401 
               
               
                 2556 
                 BLFS121 
                 136; 137; 138; 139; 140 in core and 
                 3402; 3403; 3404; 3405; 3406; 3407; 3 
               
               
                   
                   
                 homologs 
                 408; 3409; 3410; 3411; 3412; 3413; 34 
               
               
                   
                   
                   
                 14; 3415; 3416; 3417; 3418; 3419; 342 
               
               
                   
                   
                   
                 0; 3421; 3422; 3423 
               
               
                 2557 
                 BLFS122 
                 no domains in core 
               
               
                 2558 
                 BLFS123 
                 141; 8 in core and homologs 
                 3431; 3432; 3433; 3434 
               
               
                 2559 
                 BLFS124 
                 142 in core and homologs 
                 3435; 3436; 3437; 3438; 3439; 3440; 3 
               
               
                   
                   
                   
                 441; 3442; 3443; 3444; 3445; 3446; 34 
               
               
                   
                   
                   
                 47; 3448; 3449; 3450; 3451 
               
               
                 2560 
                 BLFS125 
                 72 in core and homologs 
                 3452; 3453; 3454; 3455; 3456; 3457; 3 
               
               
                   
                   
                   
                 458; 3459; 3460; 3461; 3462; 3463; 34 
               
               
                   
                   
                   
                 64; 3465; 3466; 3467 
               
               
                 2561 
                 BLFS126 
                 no domains in core 
               
               
                 2562 
                 BLFS127 
                 24 in core and homologs 
                 3476; 3477; 3478; 3479; 3480; 3481; 3 
               
               
                   
                   
                   
                 482; 3483; 3484; 3485; 3486; 3487; 34 
               
               
                   
                   
                   
                 88; 3489; 3490; 3491 
               
               
                 2563 
                 BLFS128 
                 143; 144; 145 in core and homologs 
                 3492; 3493; 3494; 3495; 3496; 3497; 3 
               
               
                   
                   
                   
                 498; 3499; 3500; 3501; 3502; 3503; 35 
               
               
                   
                   
                   
                 04; 3505; 3506; 3507; 3508 
               
               
                 2564 
                 BLFS129 
                 146; 82 in core and homologs 
                 3509; 3510; 3511; 3512; 3513; 3514; 3 
               
               
                   
                   
                   
                 515; 3516; 3517; 3518; 3519 
               
               
                 2565 
                 BLFS130 
                 147; 148; 115; 116 in core and 
                 3520; 3521; 3522; 3523; 3524 
               
               
                   
                   
                 homologs 
               
               
                 2566 
                 BLFS131 
                 83 in core and homologs 
                 3525; 3526; 3527; 3528; 3529; 3530; 3 
               
               
                   
                   
                   
                 531; 3532; 3533; 3534; 3535; 3536; 35 
               
               
                   
                   
                   
                 37; 3538; 3539; 3540; 3541; 3542; 354 
               
               
                   
                   
                   
                 3; 3544; 3545; 3546; 3547; 3548 
               
               
                 2567 
                 BLFS132 
                 no domains in core 
               
               
                 2568 
                 BLFS133 
                 no domains in core 
               
               
                 2569 
                 BLFS134 
                 149 in core and homologs 
                 3577; 3578; 3579; 3580; 3581; 3582; 3 
               
               
                   
                   
                   
                 583; 3584; 3585; 3586; 3587; 3588; 35 
               
               
                   
                   
                   
                 89; 3590; 3591; 3592; 3593; 3594; 359 
               
               
                   
                   
                   
                 5; 3596; 3597; 3598; 3599; 3600; 3601; 
               
               
                   
                   
                   
                 3602; 3603 
               
               
                 2570 
                 BLFS135 
                 60; 68 in core and homologs 
                 3604; 3605; 3606; 3607; 3608; 3609; 3 
               
               
                   
                   
                   
                 610; 3611; 3612; 3613; 3614; 3615 
               
               
                 2571 
                 BLFS136 
                 150; 151 in core and homologs 
                 3616 
               
               
                 2572 
                 BLFS137 
                 152; 153 in core and homologs 
                 3617; 3618; 3619; 3620; 3621 
               
               
                 2573 
                 BLFS138 
                 154; 155 in core and homologs 
                 3622; &#39;3623; &#39;3624; 3625; :3626; 3627; :3 
               
               
                   
                   
                   
                 628; 3629; 3630; 3631; 3632; 3633 
               
               
                 2574 
                 BLFS139 
                 31; 32; 8; 30 in core and homologs 
                 3634; 3635; 3636; 3637; 3638; 3639; 3 
               
               
                   
                   
                   
                 640; 3641; 3642; 3643; 3644; 3645; 36 
               
               
                   
                   
                   
                 46 
               
               
                 2575 
                 BLFS140 
                 156; 157; 158; 159; 24; 160 in core 
                 3647; 3648; 3649; 3650; 3651; 3652; 3 
               
               
                   
                   
                 and homologs 
                 653; 3654; 3655; 3656; 3657; 3658; 36 
               
               
                   
                   
                   
                 59; 3660 
               
               
                 2576 
                 BLFS141 
                 161; 162; 163; 24 in core and 
                 3661; 3662; 3663; 3664; 3665; 3666; 3 
               
               
                   
                   
                 homologs 
                 667; 3668; 3669; 3670; 3671; 3672; 36 
               
               
                   
                   
                   
                 73; 3674; 3675; 3676; 3677; 3678; 367 
               
               
                   
                   
                   
                 9 
               
               
                 2577 
                 BLFS142 
                 45 in core and homologs 
                 3680; 3681; 3682; 3683; 3684; 3685; 3 
               
               
                   
                   
                   
                 686; 3687; 3688; 3689; 3690 
               
               
                 2578 
                 BLFS143 
                 164; 165; 166 in core and homologs 
                 3691; 3692; 3693; 3694; 3695; 3696; 3 
               
               
                   
                   
                   
                 697; 3698; 3699; 3700; 3701; 3702; 37 
               
               
                   
                   
                   
                 03; 3704 
               
               
                 2579 
                 BLFS144 
                 72; 167; 73; 168; 169; 170 in core and 
                 3705; 3706; 3707; 3708; 3709; 3710; 3 
               
               
                   
                   
                 homologs 
                 711; 3712; 3713; 3714; 3715 
               
               
                 2580 
                 BLFS145 
                 103; 171; 172; 173 in core and 
                 3716; 3717; 3718; 3719; 3720; 3721; 3 
               
               
                   
                   
                 homologs 
                 722; 3723; 3724; 3725; 3726; 3727; 37 
               
               
                   
                   
                   
                 28; 3729 
               
               
                 2581 
                 BLFS146 
                 74; 75 in core and homologs 
                 3730; 3731; 3732; 3733; 3734; 3735; 3 
               
               
                   
                   
                   
                 736; 3737; 3738; 3739; 3740; 3741; 37 
               
               
                   
                   
                   
                 42; 3743; 3744; 3745; 3746; 3747; 374 
               
               
                   
                   
                   
                 8; 3749 
               
               
                 2582 
                 BLFS147 
                 174; 143; 39 in core and homologs 
                 3750; 3751; 3752; 3753; 3754; 3755; 3 
               
               
                   
                   
                   
                 756; 3757; 3758; 3759 
               
               
                 2583 
                 BLFS148 
                 52 in core and homologs 
                 3760; 3761; 3762; 3763; 3764; 3765; 3 
               
               
                   
                   
                   
                 766; 3767; 3768; 3769; 3770; 3771; 37 
               
               
                   
                   
                   
                 72; 3773; 3774; 3775; 3776; 3777; 377 
               
               
                   
                   
                   
                 8; 3779; 3780; 3781 
               
               
                 2584 
                 BLFS149 
                 175; 60; 61; 62; 63; 24; 64; 17; 65; 66; 67 
                 3782; 3783; 3784; 3785; 3786; 3787; 3 
               
               
                   
                   
                 in core and homologs 
                 788; 3789; 3790; 3791; 3792; 3793; 37 
               
               
                   
                   
                   
                 94; 3795; 3796; 3797 
               
               
                 2585 
                 BLFS150 
                 no domains in core 
               
               
                 2586 
                 BLFS151 
                 86; 110 in core and homologs 
                 3798; 3799; 3800 
               
               
                 2587 
                 BLFS152 
                 176; 177 in core and homologs 
                 3801; 3802 
               
               
                 2588 
                 BLFS153 
                 34; 178; 179; 180; 181 in core and 
                 3803; 3804; 3805; 3806; 3807; 3808 
               
               
                   
                   
                 homologs 
               
               
                 2589 
                 BLFS154 
                 182 in core and homologs 
                 3809; 3810 
               
               
                 2590 
                 BLFS155 
                 4; 5 in core and homologs 
                 3811; 3812 
               
               
                 2591 
                 BLFS156 
                 1; 2; 3 in core 
               
               
                 2592 
                 BLFS157 
                 99; 100; 183; 184; 6; 102 in core 
               
               
                 2593 
                 BLFS158 
                 185; 186; 187 in core and homologs 
                 3813 
               
               
                 2594 
                 BLFS159 
                 no domains in core 
               
               
                 2595 
                 PUB6 
                 188; 7; 10; 189 in core and homologs 
                 3816; 3817; 3818; 3819; 3820; 3821; 3 
               
               
                   
                   
                   
                 822; 3823; 3824; 3825 
               
               
                 2596 
                 PUB23 
                 190; 191; 7; 10; 192; 193; 194; 19 in 
               
               
                   
                   
                 core 
               
               
                 2597 
                 PUB31 
                 195; 196; 7; 10; 19 in core 
               
               
                 2598 
                 PUB65 
                 197; 15; 7; 198; 10; 82; 199; 200; 201; 2 
                 3826; 3827; 3828; 3829; 3830; 3831; 3 
               
               
                   
                   
                 02 in core and homologs 
                 832; 3833; 3834; 3835; 3836; 3837; 38 
               
               
                   
                   
                   
                 38; 3839; 3840; 3841; 3842; 3843; 384 
               
               
                   
                   
                   
                 4; 3845 
               
               
                 2599 
                 ZLFS1 
                 203; 204; 205 in core and homologs 
                 3846 
               
               
                 2600 
                 ZLFS2 
                 206 in core and homologs 
                 3847; 3848; 3849; 3850; 3851; 3852; 3 
               
               
                   
                   
                   
                 853; 3854; 3855; 3856; 3857; 3858; 38 
               
               
                   
                   
                   
                 59; 3860; 3861; 3862; 3863; 3864; 386 
               
               
                   
                   
                   
                 5 
               
               
                 2601 
                 ZLFS3 
                 31; 32; 8; 30 in core and homologs 
                 3866; 3867; 3868; 3869; 3870; 3871; 3 
               
               
                   
                   
                   
                 872; 3873; 3874; 3875; 3876; 3877; 38 
               
               
                   
                   
                   
                 78; 3879; 3880; 3881; 3882; 3883 
               
               
                 2602 
                 ZLFS5 
                 123 in core and homologs 
                 3884; 3885; 3886; 3887; 3888 
               
               
                 2603 
                 ZLFS6 
                 207; 208 in core and homologs 
                 3889; 3890; 3891; 3892; 3893 
               
               
                 2604 
                 ZLFS7 
                 209 in core and homologs 
                 3894 
               
               
                 2605 
                 ZLFS8 
                 210; 211 in core and homologs 
                 3895; 3896 
               
               
                 2606 
                 ZLFS9 
                 113; 24 in core and homologs 
                 3897 
               
               
                 2607 
                 ZLFS10 
                 no domains in core 
               
               
                 2608 
                 ZLFS11 
                 212; 213; 214 in core and homologs 
                 3898; 3899 
               
               
                 2609 
                 ZLFS12 
                 215; 216; 217; 218 in core and 
                 3900; 3901; 3902 
               
               
                   
                   
                 homologs 
               
               
                 2610 
                 ZLFS13 
                 no domains in core 
               
               
                 2611 
                 ZLFS14 
                 126 in core and homologs 
                 3905 
               
               
                 2612 
                 ZLFS15 
                 219; 52 in core and homologs 
                 3906; 3907; 3908 
               
               
                 2613 
                 ZLFS16 
                 220; 221; 103; 171; 173; 172 in core 
                 3909; 3910; 3911; 3912; 3913; 3914; 3 
               
               
                   
                   
                 and homologs 
                 915 
               
               
                 2614 
                 ZLFS17 
                 2; 1; 222 in core and homologs 
                 3916 
               
               
                 2615 
                 ZLFS18 
                 99; 100; 102 in core and homologs 
                 3917 
               
               
                 2616 
                 ZLFS19 
                 31; 74; 32; 75 in core and homologs 
                 3918; 3919; 3920; 3921; 3922; 3923; 3 
               
               
                   
                   
                   
                 924; 3925; 3926; 3927 
               
               
                 2617 
                 ZLFS21 
                 223; 101; 224 in core and homologs 
                 3928; 3929; 3930; 3931; 3932; 3933; 3 
               
               
                   
                   
                   
                 934; 3935; 3936; 3937; 3938; 3939; 39 
               
               
                   
                   
                   
                 40 
               
               
                 2618 
                 ZLFS22 
                 225; 31; 32; 226 in core and 
                 3941; 3942; 3943; 3944; 3945; 3946; 3 
               
               
                   
                   
                 homologs 
                 947; 3948; 3949; 3950; 3951; 3952; 39 
               
               
                   
                   
                   
                 53 
               
               
                 2619 
                 ZLFS23 
                 82; 92; 227 in core and homologs 
                 3954; 3955; 3956; 3957; 3958; 3959; 3 
               
               
                   
                   
                   
                 960 
               
               
                 2620 
                 ZLFS24 
                 176; 228; 229; 230; 231 in core and 
                 3961; 3962; 3963; 3964; 3965; 3966; 3 
               
               
                   
                   
                 homologs 
                 967; 3968; 3969; 3970; 3971; 3972; 39 
               
               
                   
                   
                   
                 73; 3974; 3975; 3976 
               
               
                 2621 
                 ZLFS25 
                 176; 232; 177 in core and homologs 
                 3977; 3978; 3979; 3980; 3981; 3982; 3 
               
               
                   
                   
                   
                 983 
               
               
                 2622 
                 ZLFS27 
                 31; 233; 32; 101; 234 in core and 
                 3984; 3985; 3986; 3987; 3988; 3989; 3 
               
               
                   
                   
                 homologs 
                 990; 3991; 3992; 3993; 3994; 3995; 39 
               
               
                   
                   
                   
                 96; 3997; 3998; 3999; 4000; 4001; 400 
               
               
                   
                   
                   
                 2; 4003; 4004; 4005; 4006; 4007 
               
               
                 2623 
                 ZLFS28 
                 86; 110 in core and homologs 
                 4008; 4009; 4010; 4011; 4012; 4013; 4 
               
               
                   
                   
                   
                 014; 4015; 4016; 4017; 4018; 4019; 40 
               
               
                   
                   
                   
                 20; 4021; 4022 
               
               
                 2624 
                 ZLFS29 
                 235; 219; 52 in core and homologs 
                 4023; 4024; 4025; 4026; 4027; 4028; 4 
               
               
                   
                   
                   
                 029; 4030; 4031; 4032; 4033; 4034; 40 
               
               
                   
                   
                   
                 35; 4036; 4037; 4038; 4039; 4040; 404 
               
               
                   
                   
                   
                 l; 4042; 4043; 4044; 4045; 4046; 4047; 
               
               
                   
                   
                   
                 4048 
               
               
                 2625 
                 ZLFS30 
                 136; 140; 236 in core and homologs 
                 4049; 4050; 4051; 4052; 4053; 4054; 4 
               
               
                   
                   
                   
                 055; 4056; 4057; 4058; 4059; 4060; 40 
               
               
                   
                   
                   
                 61; 4062; 4063; 4064; 4065; 4066; 406 
               
               
                   
                   
                   
                 7; 4068 
               
               
                 2626 
                 ZLFS31 
                 228; 230; 229; 231 in core and 
                 4069; 4070; 4071; 4072; 4073; 4074; 4 
               
               
                   
                   
                 homologs 
                 075; 4076; 4077; 4078; 4079; 4080; 40 
               
               
                   
                   
                   
                 81 
               
               
                 2627 
                 ZLFS32 
                 237; 238; 239 in core and homologs 
                 4082; 4083; 4084; 4085; 4086; 4087; 4 
               
               
                   
                   
                   
                 088; 4089; 4090; 4091; 4092; 4093; 40 
               
               
                   
                   
                   
                 94; 4095; 4096; 4097 
               
               
                 2628 
                 ZLFS33 
                 60; 68 in core and homologs 
                 4098; 4099 
               
               
                 2629 
                 ZLFS34 
                 240; 241; 242 in core and homologs 
                 4100; 4101; 4102; 4103; 4104; 4105; 4 
               
               
                   
                   
                   
                 106; 4107; 4108; 4109; 4110; 4111; 41 
               
               
                   
                   
                   
                 12; 4113; 4114; 4115; 4116; 4117; 411 
               
               
                   
                   
                   
                 8; 4119 
               
               
                 2630 
                 ZLFS35 
                 no domains in core 
               
               
                 2631 
                 ZLFS36 
                 no domains in core 
               
               
                 2632 
                 ZLFS37 
                 243; 244 in core and homologs 
                 4126; 4127; 4128; 4129; 4130; 4131; 4 
               
               
                   
                   
                   
                 132; 4133; 4134; 4135; 4136; 4137; 41 
               
               
                   
                   
                   
                 38; 4139; 4140; 4141; 4142; 4143; 414 
               
               
                   
                   
                   
                 4; 4145; 4146; 4147; 4148; 4149; 4150; 
               
               
                   
                   
                   
                 4151; 4152; 4153 
               
               
                 2633 
                 ZLFS38 
                 245; 246; 247; 248; 249 in core and 
                 4154; 4155; 4156; 4157; 4158; 4159; 4 
               
               
                   
                   
                 homologs 
                 160; 4161; 4162; 4163; 4164; 4165; 41 
               
               
                   
                   
                   
                 66; 4167; 4168; 4169; 4170; 4171; 417 
               
               
                   
                   
                   
                 2; 4173; 4174; 4175; 4176 
               
               
                 2634 
                 ZLFS39 
                 250; 60; 251; 24; 71; 17; 252; 111 in 
                 4177; 4178; 4179; 4180; 4181; 4182; 4 
               
               
                   
                   
                 core and homologs 
                 183; 4184; 4185; 4186; 4187; 4188; 41 
               
               
                   
                   
                   
                 89; 4190; 4191; 4192; 4193; 4194; 419 
               
               
                   
                   
                   
                 5; 4196; 4197; 4198; 4199; 4200; 4201 
               
               
                 2635 
                 ZLFS42 
                 1; 2; 3 in core and homologs 
                 2637; 2660; 4202; 4203; 4204; 4205; 4 
               
               
                   
                   
                   
                 206; 4207; 4208; 4209; 4210 
               
               
                 2636 
                 ZLFS44 
                 8; 30 in core and homologs 
                 4211; 4212; 4213; 4214; 4215; 4216; 4 
               
               
                   
                   
                   
                 217; 4218; 4219; 4220; 4221; 4222; 42 
               
               
                   
                   
                   
                 23; 4224; 4225; 4226; 4227; 4228; 422 
               
               
                   
                   
                   
                 9; 4230; 4231 
               
               
                 2637 
                 ZLFS45 
                 2; 1; 3 in core and homologs 
                 4202; 4203; 4204; 4205; 4206; 4207; 4 
               
               
                   
                   
                   
                 208; 4209; 4210; 4232 
               
               
                 2638 
                 ZLFS47 
                 2; 1; 222; 92 in core 
               
               
                 2639 
                 ZLFS48 
                 2; 1; 222 in core and homologs 
                 3916 
               
               
                 2640 
                 ZLFS49 
                 4; 74; 5 in core and homologs 
                 4233; 4234 
               
               
                 2641 
                 ZLFS51 
                 31; 40; 41; 42; 32; 24 in core and 
                 4235; 4236; 4237; 4238; 4239; 4240; 4 
               
               
                   
                   
                 homologs 
                 241; 4242; 4243; 4244; 4245; 4246; 42 
               
               
                   
                   
                   
                 47; 4248; 4249; 4250; 4251; 4252 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 18 
               
             
            
               
                   
               
               
                 Details of identified domains presented in Table 17 
               
            
           
           
               
               
               
               
            
               
                 Domain 
                 InterPro 
                   
                   
               
               
                 Identifier 
                 No. 
                 Accession No. 
                 Description 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 1 
                 IPR011024 
                 SSF49695 
                 Gamma-crystallin-like 
               
               
                 2 
                 IPR015791 
                 G3DSA:2.60.20.30 
                 Antimicrobial/protein inhibitor, gamma- 
               
               
                   
                   
                   
                 crystallin-like 
               
               
                 3 
                 IPR015161 
                 PF09076 
                 Beta/Gamma crystallin  Streptomyces  killer 
               
               
                   
                   
                   
                 toxin-like, beta/gamma crystallin 
               
               
                 4 
                 IPR001480 
                 PS50927 
                 Bulb-type lectin domain profile. Bulb-type lectin 
               
               
                   
                   
                   
                 domain 
               
               
                 5 
                 IPR036426 
                 G3DSA:2.90.10.10 
                 Bulb-type lectin domain superfamily 
               
               
                 6 
                 IPR004991 
                 PF03318 
                 Clostridium epsilon toxin ETX/ Bacillus   
               
               
                   
                   
                   
                 mosquitocidal toxin MTX2 Aerolysin-like toxin 
               
               
                 7 
                 IPR035992 
                 SSF50370 
                 Ricin B-like lectins 
               
               
                 8 
                 IPR038765 
                 SSF54001 
                 Papain-like cysteine peptidase superfamily 
               
               
                 9 
                 IPR013688 
                 PF08481 
                 GBS Bsp-like repeat GBS Bsp-like 
               
               
                 10 
                 IPR000772 
                 cd00161 
                 RICIN Ricin B, lectin domain 
               
               
                 11 
                 IPR008999 
                 SSF50405 
                 Actin-crosslinking 
               
               
                 12 
                 IPR039564 
                 PF13529 
                 Peptidase C39 like family Peptidase C39-like 
               
               
                 13 
                 IPR001322 
                 PF00932 
                 Lamin Tail Domain Lamin tail domain 
               
               
                 14 
                 IPR036415 
                 SSF74853 
                 Lamin tail domain superfamily 
               
               
                 15 
                 IPR032812 
                 PF13205 
                 Bacterial Ig-like domain SbsA, Ig-like domain 
               
               
                 16 
                 IPR005077 
                 PF03415 
                 Clostripain family Peptidase C11, clostripain 
               
               
                 17 
                 IPR013783 
                 G3DSA:2.60.40.10 
                 Immunoglobulin-like fold 
               
               
                 18 
                 IPR003737 
                 PF02585 
                 GlcNAc-PI de-N-acetylase N- 
               
               
                   
                   
                   
                 acetylglucosaminyl phosphatidylinositol 
               
               
                   
                   
                   
                 deacetylase-related 
               
               
                 19 
                 IPR008979 
                 G3DSA:2.60.120.260 
                 Galactose-binding-like domain superfamily 
               
               
                 20 
                 IPR024078 
                 SSF102588 
                 Putative deacetylase LmbE-like domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 21 
                 IPR008270 
                 PS00953 
                 Glycosyl hydrolases family 25 active sites 
               
               
                   
                   
                   
                 signature. Glycoside hydrolase, family 25, active 
               
               
                   
                   
                   
                 site 
               
               
                 22 
                 IPR002053 
                 PF01183 
                 Glycosyl hydrolases family 25 Glycoside 
               
               
                   
                   
                   
                 hydrolase, family 25 
               
               
                 23 
                 IPRO 18077 
                 SM00641 
                 Glycoside hydrolase, family 25 subgroup 
               
               
                 24 
                 IPR017853 
                 SSF51445 
                 Glycoside hydrolase superfamily 
               
               
                 25 
                 IPR009045 
                 G3DSA:3.30.1380.10 
                 Hedgehog signalling/DD-peptidase zinc- 
               
               
                   
                   
                   
                 binding domain superfamily 
               
               
                 26 
                 IPR003709 
                 PF02557 
                 D-alanyl-D-alanine carboxypeptidase Peptidase 
               
               
                   
                   
                   
                 M15B 
               
               
                 27 
                 IPRO 12340 
                 SSF50249 
                 Nucleic acid-binding, OB-fold 
               
               
                 28 
                 IPR003029 
                 PS50126 
                 S1 domain profile. S1 domain 
               
               
                 29 
                 IPR022967 
                 SM00316 
                 RNA-binding domain, S1 
               
               
                 30 
                 IPR000064 
                 PF00877 
                 NlpC/P60 family Endopeptidase, NLPC/P60 
               
               
                   
                   
                   
                 domain 
               
               
                 31 
                 IPRO 18392 
                 SM00257 
                 LysM domain 
               
               
                 32 
                 IPR036779 
                 G3DSA:3.10.350.10 
                 LysM domain superfamily 
               
               
                 33 
                 IPR022887 
                 MF_02014 
                 Cell division suppressor protein YneA [yneA], 
               
               
                   
                   
                   
                 Cell division suppressor protein, YneA 
               
               
                 34 
                 IPR009003 
                 SSF50494 
                 Peptidase S1, PA clan 
               
               
                 35 
                 IPR036034 
                 SSF50156 
                 PDZ superfamily 
               
               
                 36 
                 IPR008763 
                 PF05580 
                 SpoIVB peptidase S55 Peptidase S55, SpoIVB 
               
               
                 37 
                 IPR001478 
                 PS50106 
                 PDZ domain profile. PDZ domain 
               
               
                 38 
                 IPR014219 
                 TIGR02860 
                 spore_IV_B: stage IV sporulation protein B 
               
               
                   
                   
                   
                 Peptidase S55, sporulation stage IV, protein B 
               
               
                 39 
                 IPR003646 
                 PF08239 
                 Bacterial SH3 domain SH3-like domain, 
               
               
                   
                   
                   
                 bacterial-type 
               
               
                 40 
                 IPR001223 
                 PF00704 
                 Glycosyl hydrolases family 18 Glycoside 
               
               
                   
                   
                   
                 hydrolase family 18, catalytic domain 
               
               
                 41 
                 IPR011583 
                 SM00636 
                 Chitinase II 
               
               
                 42 
                 IPR029070 
                 G3DSA:3.10.50.10 
                 Chitinase insertion domain superfamily 
               
               
                 43 
                 IPR011330 
                 SSF88713 
                 Glycoside hydrolase/deacetylase, beta/alpha- 
               
               
                   
                   
                   
                 barrel 
               
               
                 44 
                 IPR002509 
                 PS51677 
                 NodB homology domain profile. NodB 
               
               
                   
                   
                   
                 homology domain 
               
               
                 45 
                 IPR011105 
                 PF07486 
                 Cell Wall Hydrolase Cell wall hydrolase, SleB 
               
               
                 46 
                 IPR010310 
                 PF06013 
                 Proteins of 100 residues with WXG Type VII 
               
               
                   
                   
                   
                 secretion system ESAT-6-like 
               
               
                 47 
                 IPR037146 
                 G3DSA:3.90.540.10 
                 Colicin/pyocin, DNase domain superfamily 
               
               
                 48 
                 IPR036689 
                 SSF140453 
                 ESAT-6-like superfamily 
               
               
                 49 
                 IPR001119 
                 PF00395 
                 S-layer homology domain S-layer homology 
               
               
                   
                   
                   
                 domain 
               
               
                 50 
                 IPR019730 
                 PF10794 
                 Protein of unknown function (DUF2606) Protein 
               
               
                   
                   
                   
                 of unknown function DUF2606 
               
               
                 51 
                 IPR013784 
                 SSF49452 
                 Carbohydrate-binding-like fold 
               
               
                 52 
                 IPR001915 
                 PF01435 
                 Peptidase family M48 Peptidase M48 
               
               
                 53 
                 IPR008915 
                 PF02163 
                 Peptidase family M50 Peptidase M50 
               
               
                 54 
                 IPR004387 
                 TIGR00054 
                 TIGR00054: RIP metalloprotease RseP 
               
               
                   
                   
                   
                 Peptidase M50, putative membrane-associated 
               
               
                   
                   
                   
                 zinc metallopeptidase 
               
               
                 55 
                 IPR025493 
                 PF14326 
                 Domain of unknown function (DUF4384) 
               
               
                   
                   
                   
                 Domain of unknown function DUF4384 
               
               
                 56 
                 IPR015059 
                 PF08964 
                 Beta/Gamma crystallin Calcium-dependent cell 
               
               
                   
                   
                   
                 adhesion molecule, N-terminal 
               
               
                 57 
                 IPR001064 
                 PS50915 
                 Crystallins beta and gamma ‘Greek key’ motif 
               
               
                   
                   
                   
                 profile. Beta/gamma crystallin 
               
               
                 58 
                 IPR036404 
                 G3DSA:2.100.10.30 
                 Jacalin-like lectin domain superfamily 
               
               
                 59 
                 IPR001229 
                 PS51752 
                 Jacalin-type lectin domain profile. Jacalin-like 
               
               
                   
                   
                   
                 lectin domain 
               
               
                 60 
                 IPR014756 
                 SSF81296 
                 Immunoglobulin E-set 
               
               
                 61 
                 IPR015883 
                 PF00728 
                 Glycosyl hydrolase family 20, catalytic domain 
               
               
                   
                   
                   
                 Glycoside hydrolase family 20, catalytic domain 
               
               
                 62 
                 IPR015882 
                 PF02838 
                 Glycosyl hydrolase family 20, domain 2 Beta- 
               
               
                   
                   
                   
                 hexosaminidase, bacterial type, N-terminal 
               
               
                 63 
                 IPR012291 
                 G3DSA:2.60.40.290 
                 CBM2, carbohydrate-binding domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 64 
                 IPR029018 
                 G3DSA:3.30.379.10 
                 Beta-hexosaminidase-like, domain 2 
               
               
                 65 
                 IPR025705 
                 PR00738 
                 Glycosyl hydrolase family 20 signature Beta- 
               
               
                   
                   
                   
                 hexosaminidase 
               
               
                 66 
                 IPR008965 
                 SSF49384 
                 CBM2/CBM3, carbohydrate-binding domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 67 
                 IPR004866 
                 PF03173 
                 Putative carbohydrate binding domain 
               
               
                   
                   
                   
                 Chitobiase/beta-hexosaminidases, N-terminal 
               
               
                   
                   
                   
                 domain 
               
               
                 68 
                 IPR004302 
                 PF03067 
                 Lytic polysaccharide mono-oxygenase, 
               
               
                   
                   
                   
                 cellulose-degrading Cellulose/chitin-binding 
               
               
                   
                   
                   
                 protein, N-terminal 
               
               
                 69 
                 IPR013785 
                 G3DSA:3.20.20.70 
                 Aldolase-type TIM barrel 
               
               
                 70 
                 IPR002241 
                 PF16499 
                 Alpha galactosidase A Glycoside hydrolase, 
               
               
                   
                   
                   
                 family 27 
               
               
                 71 
                 IPR013780 
                 G3DSA:2.60.40.1180 
                 Glycosyl hydrolase, all-beta 
               
               
                 72 
                 IPR025711 
                 PF13670 
                 Peptidase propeptide and YPEB domain PepSY 
               
               
                   
                   
                   
                 domain 
               
               
                 73 
                 IPR011096 
                 PF07504 
                 Fungalysin/Thermolysin Propeptide Motif FTP 
               
               
                   
                   
                   
                 domain 
               
               
                 74 
                 IPR011055 
                 G3DSA:2.70.70.10 
                 Duplicated hybrid motif 
               
               
                 75 
                 IPR016047 
                 PF01551 
                 Peptidase family M23 Peptidase M23 
               
               
                 76 
                 IPR001547 
                 PF00150 
                 Cellulase (glycosyl hydrolase family 5) 
               
               
                   
                   
                   
                 Glycoside hydrolase, family 5 
               
               
                 77 
                 IPR007484 
                 PF04389 
                 Peptidase family M28 Peptidase M28 
               
               
                 78 
                 IPR036116 
                 SSF49265 
                 Fibronectin type III superfamily 
               
               
                 79 
                 IPR003961 
                 cd00063 
                 FN3 Fibronectin type III 
               
               
                 80 
                 IPR002594 
                 PF01670 
                 Glycosyl hydrolase family 12 Glycoside 
               
               
                   
                   
                   
                 hydrolase family 12 
               
               
                 81 
                 IPR013319 
                 G3DSA:2.60.120.180 
                 Glycoside hydrolase family 11/12 
               
               
                 82 
                 IPR013320 
                 SSF49899 
                 Concanavalin A-like lectin/glucanase domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 83 
                 IPR008816 
                 PF05433 
                 Glycine zipper 2TM domain Glycine zipper 
               
               
                   
                   
                   
                 2TM domain 
               
               
                 84 
                 IPR011049 
                 SSF51120 
                 Serralysin-like metalloprotease, C-terminal 
               
               
                 85 
                 IPR001343 
                 PF00353 
                 RTX calcium-binding nonapeptide repeat (4 
               
               
                   
                   
                   
                 copies) RTX calcium-binding nonapeptide 
               
               
                   
                   
                   
                 repeat 
               
               
                 86 
                 IPR029058 
                 G3DSA:3.40.50.1820 
                 Alpha/Beta hydrolase fold 
               
               
                 87 
                 IPR003995 
                 PR01488 
                 Gram-negative bacterial RTX toxin determinant 
               
               
                   
                   
                   
                 A family signature RTX toxin determinant A 
               
               
                 88 
                 IPR018511 
                 PS00330 
                 Hemolysin-type calcium-binding region 
               
               
                   
                   
                   
                 signature. Hemolysin-type calcium-binding 
               
               
                   
                   
                   
                 conserved site 
               
               
                 89 
                 IPR021731 
                 PF11741 
                 AMIN domain AMIN domain 
               
               
                 90 
                 IPR002508 
                 cd02696 
                 MurNAc-LAA N-acetylmuramoyl-L-alanine 
               
               
                   
                   
                   
                 amidase, catalytic domain 
               
               
                 91 
                 IPR014262 
                 TIGR02913 
                 HAF_rpt: probable extracellular repeat, HAF 
               
               
                   
                   
                   
                 family Extracellular HAF 
               
               
                 92 
                 IPR006311 
                 PS51318 
                 Twin arginine translocation (Tat) signal profile. 
               
               
                   
                   
                   
                 Twin-arginine translocation pathway, signal 
               
               
                   
                   
                   
                 sequence 
               
               
                 93 
                 IPR011050 
                 SSF51126 
                 Pectin lyase fold/virulence factor 
               
               
                 94 
                 IPR024973 
                 PF13018 
                 Extended Signal Peptide of Type V secretion 
               
               
                   
                   
                   
                 system ESPR domain 
               
               
                 95 
                 IPR008638 
                 TIGR01901 
                 adhes_NPXG: filamentous hemagglutinin 
               
               
                   
                   
                   
                 family N-terminal domain Filamentous 
               
               
                   
                   
                   
                 haemagglutinin, N-terminal 
               
               
                 96 
                 IPR011493 
                 PF07581 
                 The GLUG motif GLUG 
               
               
                 97 
                 IPR012334 
                 G3DSA:2.160.20.10 
                 Pectin lyase fold 
               
               
                 98 
                 IPR003540 
                 PF03496 
                 ADP-ribosyltransferase exoenzyme ADP 
               
               
                   
                   
                   
                 ribosyltransferase 
               
               
                 99 
                 IPR036365 
                 SSF47090 
                 PGBD-like superfamily 
               
               
                 100 
                 IPR002477 
                 PF01471 
                 Putative peptidoglycan binding domain 
               
               
                   
                   
                   
                 Peptidoglycan binding-like 
               
               
                 101 
                 IPR023346 
                 SSF53955 
                 Lysozyme-like domain superfamily 
               
               
                 102 
                 IPR036366 
                 G3DSA:1.10.101.10 
                 PGBD superfamily 
               
               
                 103 
                 IPR012338 
                 SSF56601 
                 Beta-lactamase/transpeptidase-like 
               
               
                 104 
                 IPR001466 
                 PF00144 
                 Beta-lactamase Beta-lactamase-related 
               
               
                 105 
                 IPR014235 
                 cd10948 
                 CE4_BsPdaA_like Peptidoglycan-N- 
               
               
                   
                   
                   
                 acetylmuramic acid deacetylase PdaA 
               
               
                 106 
                 IPR036434 
                 SSF51989 
                 1,4-beta cellobiohydrolase superfamily 
               
               
                 107 
                 IPR016288 
                 PF01341 
                 Glycosyl hydrolases family 6 1,4-beta 
               
               
                   
                   
                   
                 cellobiohydrolase 
               
               
                 108 
                 IPR036966 
                 G3DSA:2.60.40.710 
                 Carbohydrate-binding module 3 superfamily 
               
               
                 109 
                 IPR001956 
                 PS51172 
                 CBM3 (carbohydrate binding type-3) domain 
               
               
                   
                   
                   
                 profile. Carbohydrate-binding module 3 
               
               
                 110 
                 IPR000073 
                 PF12697 
                 Alpha/beta hydrolase family Alpha/beta 
               
               
                   
                   
                   
                 hydrolase fold-1 
               
               
                 111 
                 IPR006047 
                 SM00642 
                 Glycosyl hydrolase, family 13, catalytic domain 
               
               
                 112 
                 IPR016282 
                 PIRSF001043 
                 Glycoside hydrolase, family 5, endoglucanase B 
               
               
                 113 
                 IPR018087 
                 PS00659 
                 Glycosyl hydrolases family 5 signature. 
               
               
                   
                   
                   
                 Glycoside hydrolase, family 5, conserved site 
               
               
                 114 
                 IPR005102 
                 PF03442 
                 Carbohydrate binding domain X2 Carbohydrate 
               
               
                   
                   
                   
                 binding X2 domain 
               
               
                 115 
                 IPR001611 
                 PS51450 
                 Leucine-rich repeat profile. Leucine-rich repeat 
               
               
                 116 
                 IPR032675 
                 G3DSA:3.80.10.10 
                 Leucine-rich repeat domain superfamily 
               
               
                 117 
                 IPR011683 
                 PF07745 
                 Glycosyl hydrolase family 53 Glycosyl 
               
               
                   
                   
                   
                 hydrolase family 53 
               
               
                 118 
                 IPR034641 
                 cd10318 
                 RGL11 Polysaccharide lyase family 11 
               
               
                 119 
                 IPR020864 
                 PF01823 
                 MAC/Perforin domain Membrane attack 
               
               
                   
                   
                   
                 complex component/perforin (MACPF) domain 
               
               
                 120 
                 IPR010854 
                 PF07338 
                 Protein of unknown function (DUF1471) 
               
               
                   
                   
                   
                 Domain of unknown function DUF1471 
               
               
                 121 
                 IPR036275 
                 SSF159871 
                 YdgH-like superfamily 
               
               
                 122 
                 IPR025543 
                 G3DSA:3.30.1660.10 
                 Dodecin-like 
               
               
                 123 
                 IPR009739 
                 PF07007 
                 Lysozyme inhibitor LprI Lysozyme inhibitor 
               
               
                   
                   
                   
                 LprI, N-terminal 
               
               
                 124 
                 IPR010279 
                 PF05957 
                 Bacterial protein of unknown function 
               
               
                   
                   
                   
                 (DUF883) Inner membrane protein YqjD/ElaB 
               
               
                 125 
                 IPR007893 
                 SM00972 
                 Spore coat protein U 
               
               
                 126 
                 IPR009241 
                 PF05973 
                 Phage derived protein Gp49-like (DUF891) 
               
               
                   
                   
                   
                 Toxin HigB-like 
               
               
                 127 
                 IPR006914 
                 PF04829 
                 Pre-toxin domain with VENN motif VENN 
               
               
                   
                   
                   
                 motif-containing domain 
               
               
                 128 
                 IPR013728 
                 PF08522 
                 Domain of unknown function (DUF1735) 
               
               
                   
                   
                   
                 Domain of unknown function DUF1735 
               
               
                 129 
                 IPR003848 
                 cd06259 
                 YdcF-like Domain of unknown function 
               
               
                   
                   
                   
                 DUF218 
               
               
                 130 
                 IPR005490 
                 PF03734 
                 L,D-transpeptidase catalytic domain L,D- 
               
               
                   
                   
                   
                 transpeptidase catalytic domain 
               
               
                 131 
                 IPR038063 
                 SSF141523 
                 L,D-transpeptidase catalytic domain-like 
               
               
                 132 
                 IPR021109 
                 SSF50630 
                 Aspartic peptidase domain superfamily 
               
               
                 133 
                 IPR001940 
                 PR00834 
                 HtrA/DegQ protease family signature Peptidase 
               
               
                   
                   
                   
                 S1C 
               
               
                 134 
                 IPR025425 
                 PF13665 
                 Domain of unknown function (DUF4150) 
               
               
                   
                   
                   
                 Domain of unknown function DUF4150 
               
               
                 135 
                 IPR028917 
                 PF15635 
                 GHH signature containing HNH/Endo VII 
               
               
                   
                   
                   
                 superfamily nuclease toxin 2 Tox-GHH2 
               
               
                   
                   
                   
                 domain 
               
               
                 136 
                 IPR002933 
                 PF01546 
                 Peptidase family M20/M25/M40 Peptidase M20 
               
               
                 137 
                 IPR001261 
                 PS00758 
                 ArgE/dapE/ACY1/CPG2/yscS family 
               
               
                   
                   
                   
                 signature 1. ArgE/DapE/ACY1/CPG2/YscS. 
               
               
                   
                   
                   
                 conserved site 
               
               
                 138 
                 IPR017150 
                 cd03885 
                 M20_CPDG2 Peptidase M20, glutamate 
               
               
                   
                   
                   
                 carboxypeptidase 
               
               
                 139 
                 IPR011650 
                 PF07687 
                 Peptidase dimerisation domain Peptidase M20, 
               
               
                   
                   
                   
                 dimerisation domain 
               
               
                 140 
                 IPR036264 
                 SSF55031 
                 Bacterial exopeptidase dimerisation domain 
               
               
                 141 
                 IPR000668 
                 PF00112 
                 Papain family cysteine protease Peptidase C1A, 
               
               
                   
                   
                   
                 papain C-terminal 
               
               
                 142 
                 IPR012661 
                 PF09498 
                 Protein of unknown function (DUF2388) 
               
               
                   
                   
                   
                 Conserved hypothetical protein CHP02448 
               
               
                 143 
                 IPR002901 
                 PF01832 
                 Mannosyl-glycoprotein endo-beta-N- 
               
               
                   
                   
                   
                 acetylglucosaminidase Mannosyl-glycoprotein 
               
               
                   
                   
                   
                 endo-beta-N-acetylglucosamidase-like domain 
               
               
                 144 
                 IPR013377 
                 TIGR02541 
                 flagell_FlgJ: flagellar rod assembly 
               
               
                   
                   
                   
                 protein/muramidase FlgJ Peptidoglycan 
               
               
                   
                   
                   
                 hydrolase FlgJ 
               
               
                 145 
                 IPR019301 
                 PF10135 
                 Rod binding protein Flagellar protein FlgJ, N- 
               
               
                   
                   
                   
                 terminal 
               
               
                 146 
                 IPR014895 
                 PF08787 
                 Alginate lyase Alginate lyase 2 
               
               
                 147 
                 IPR029487 
                 PF14496 
                 C-terminal novel E3 ligase, LRR-interacting 
               
               
                   
                   
                   
                 Novel E3 ligase domain 
               
               
                 148 
                 IPR003591 
                 SM00369 
                 Leucine-rich repeat, typical subtype 
               
               
                 149 
                 IPR021357 
                 PF11191 
                 Protein of unknown function (DUF2782) Protein 
               
               
                   
                   
                   
                 of unknown function DUF2782 
               
               
                 150 
                 IPR007055 
                 PS50914 
                 BON domain profile. BON domain 
               
               
                 151 
                 IPR014004 
                 SM00749 
                 Transport-associated and nodulation domain, 
               
               
                   
                   
                   
                 bacteria 
               
               
                 152 
                 IPR015286 
                 PF09203 
                 MspA Porin family, mycobacterial-type 
               
               
                 153 
                 IPR036435 
                 SSF56959 
                 Leukocidin/porin MspA superfamily 
               
               
                 154 
                 IPR005320 
                 cd03146 
                 GAT1_Peptidase_E Peptidase S51 
               
               
                 155 
                 IPR029062 
                 G3DSA:3.40.50.880 
                 Class I glutamine amidotransferase-like 
               
               
                 156 
                 IPR001764 
                 PF00933 
                 Glycosyl hydrolase family 3 N terminal domain 
               
               
                   
                   
                   
                 Glycoside hydrolase, family 3, N-terminal 
               
               
                 157 
                 IPR002772 
                 PF01915 
                 Glycosyl hydrolase family 3 C-terminal domain 
               
               
                   
                   
                   
                 Glycoside hydrolase family 3 C-terminal 
               
               
                   
                   
                   
                 domain 
               
               
                 158 
                 IPR036962 
                 G3DSA:3.20.20.300 
                 Glycoside hydrolase, family 3, N-terminal 
               
               
                   
                   
                   
                 domain superfamily 
               
               
                 159 
                 IPR019800 
                 PS00775 
                 Glycosyl hydrolases family 3 active site. 
               
               
                   
                   
                   
                 Glycoside hydrolase, family 3, active site 
               
               
                 160 
                 IPR036881 
                 SSF52279 
                 Glycoside hydrolase family 3 C-terminal 
               
               
                   
                   
                   
                 domain superfamily 
               
               
                 161 
                 IPR016714 
                 PIRSF018168 
                 Mannan endo-1,4-beta-mannosidase 
               
               
                 162 
                 IPR000805 
                 PR00739 
                 Glycosyl hydrolase family 26 signature 
               
               
                   
                   
                   
                 Glycoside hydrolase family 26 
               
               
                 163 
                 IPR022790 
                 PF02156 
                 Glycosyl hydrolase family 26 Glycosyl 
               
               
                   
                   
                   
                 hydrolase family 26 domain 
               
               
                 164 
                 IPR029045 
                 SSF52096 
                 ClpP/crotonase-like domain superfamily 
               
               
                 165 
                 IPR002142 
                 PF01343 
                 Peptidase family S49 Peptidase S49 
               
               
                 166 
                 IPR004635 
                 TIGR00706 
                 SppA_dom: signal peptide peptidase SppA, 36K 
               
               
                   
                   
                   
                 type Peptidase S49, SppA 
               
               
                 167 
                 IPR027268 
                 G3DSA:1.10.390.10 
                 Peptidase M4/M1, CTD superfamily 
               
               
                 168 
                 IPR001570 
                 PF02868 
                 Thermolysin metallopeptidase, alpha-helical 
               
               
                   
                   
                   
                 domain Peptidase M4, C-terminal 
               
               
                 169 
                 IPR013856 
                 PF01447 
                 Thermolysin metallopeptidase, catalytic domain 
               
               
                   
                   
                   
                 Peptidase M4 domain 
               
               
                 170 
                 IPR023612 
                 PR00730 
                 Thermolysin metalloprotease (M4) family 
               
               
                   
                   
                   
                 signature Peptidase M4 
               
               
                 171 
                 IPR012907 
                 PF07943 
                 Penicillin-binding protein 5, C-terminal domain 
               
               
                   
                   
                   
                 Peptidase S11, D-Ala-D-Alacarboxypeptidase 
               
               
                   
                   
                   
                 A, C-terminal 
               
               
                 172 
                 IPR018044 
                 PR00725 
                 D-Ala-D-Alacarboxypeptidase 1 (S11) family 
               
               
                   
                   
                   
                 signature Peptidase S11, D-alanyl-D-alanine 
               
               
                   
                   
                   
                 carboxypeptidase A 
               
               
                 173 
                 IPR001967 
                 PF00768 
                 D-alanyl-D-alanine carboxypeptidase Peptidase 
               
               
                   
                   
                   
                 S11, D-alanyl-D-alanine carboxypeptidase A, N- 
               
               
                   
                   
                   
                 terminal 
               
               
                 174 
                 IPR007730 
                 PS51724 
                 SPOR domain profile. Sporulation-like domain 
               
               
                 175 
                 IPR004867 
                 cd02847 
                 E_set_Chitobiase_C Chitobiase C-terminal 
               
               
                   
                   
                   
                 domain 
               
               
                 176 
                 IPR036514 
                 G3DSA:3.40.50.1110 
                 SGNH hydrolase superfamily 
               
               
                 177 
                 IPR013830 
                 PF13472 
                 GDSL-like Lipase/Acylhydrolase family SGNH 
               
               
                   
                   
                   
                 hydrolase-type esterase domain 
               
               
                 178 
                 IPR001316 
                 PR00861 
                 Alpha-lytic endopeptidase serine protease (S2A) 
               
               
                   
                   
                   
                 signature Peptidase S1A, streptogrisin 
               
               
                 179 
                 IPR033116 
                 PS00135 
                 Serine proteases, trypsin family, serine active 
               
               
                   
                   
                   
                 site. Serine proteases, trypsin family, serine 
               
               
                   
                   
                   
                 active site 
               
               
                 180 
                 IPR004236 
                 PF02983 
                 Alpha-lytic protease prodomain Peptidase S1A, 
               
               
                   
                   
                   
                 alpha-lytic prodomain 
               
               
                 181 
                 IPR001254 
                 PF00089 
                 Trypsin Serine proteases, trypsin domain 
               
               
                 182 
                 IPR007921 
                 PF05257 
                 CHAP domain CHAP domain 
               
               
                 183 
                 IPR006616 
                 SM00696 
                 DM9 repeat 
               
               
                 184 
                 IPR024518 
                 PF11901 
                 Protein of unknown function (DUF3421) 
               
               
                   
                   
                   
                 Domain of unknown function DUF3421 
               
               
                 185 
                 IPR011051 
                 SSF51182 
                 RmlC-like cupin domain superfamily 
               
               
                 186 
                 IPR006045 
                 PF00190 
                 Cupin Cupin 1 
               
               
                 187 
                 IPR014710 
                 G3DSA:2.60.120.10 
                 RmlC-like jelly roll fold 
               
               
                 188 
                 IPR001434 
                 PF01345 
                 Domain of unknown function DUF11 Domain 
               
               
                   
                   
                   
                 of unknown function DUF11 
               
               
                 189 
                 IPR008966 
                 SSF49401 
                 Adhesion domain superfamily 
               
               
                 190 
                 IPR036399 
                 G3DSA:2.100.10.10 
                 Pesticidal crystal protein, central domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 191 
                 IPR005639 
                 PF03945 
                 delta endotoxin, N-terminal domain Pesticidal 
               
               
                   
                   
                   
                 crystal protein, N-terminal 
               
               
                 192 
                 IPR001178 
                 PF00555 
                 delta endotoxin Pesticidal crystal protein, central 
               
               
                   
                   
                   
                 domain 
               
               
                 193 
                 IPR005638 
                 PF03944 
                 delta endotoxin Pesticidal crystal protein, C- 
               
               
                   
                   
                   
                 terminal 
               
               
                 194 
                 IPR036716 
                 SSF56849 
                 Pesticidal crystal protein, N-terminal domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 195 
                 IPR000909 
                 PF00388 
                 Phosphatidylinositol-specific phospholipase C, 
               
               
                   
                   
                   
                 X domain Phosphatidylinositol-specific 
               
               
                   
                   
                   
                 phospholipase C, X domain 
               
               
                 196 
                 IPR017946 
                 SSF51695 
                 PLC-like phosphodiesterase, TIM beta/alpha- 
               
               
                   
                   
                   
                 barrel domain superfamily 
               
               
                 197 
                 IPR022409 
                 SM00089 
                 PKD/Chitinase domain 
               
               
                 198 
                 IPR000601 
                 PF00801 
                 PKD domain PKD domain 
               
               
                 199 
                 IPR036909 
                 SSF46626 
                 Cytochrome c-like domain superfamily 
               
               
                 200 
                 IPR015943 
                 G3DSA:2.130.10.10 
                 WD40/YVTN repeat-like-containing domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 201 
                 IPR009056 
                 PS51007 
                 Cytochrome c family profile. Cytochrome c-like 
               
               
                   
                   
                   
                 domain 
               
               
                 202 
                 IPR035986 
                 SSF49299 
                 PKD domain superfamily 
               
               
                 203 
                 IPR036574 
                 G3DSA:3.30.30.10 
                 Knottin, scorpion toxin-like superfamily 
               
               
                 204 
                 IPR003614 
                 cd00107 
                 Knot1 Knottin, scorpion toxin-like 
               
               
                 205 
                 IPR001542 
                 PF01097 
                 Arthropod defensin Defensin, 
               
               
                   
                   
                   
                 invertebrate/fungal 
               
               
                 206 
                 IPR000834 
                 PS00132 
                 Zinc carboxypeptidases, zinc-binding region 1 
               
               
                   
                   
                   
                 signature. Peptidase M14, carboxypeptidase A 
               
               
                 207 
                 IPR007048 
                 PF04965 
                 Gene 25-like lysozyme GpW/Gp25/IraD 
               
               
                 208 
                 IPR017737 
                 TIGR03357 
                 VI_zyme: type VI secretion system lysozyme- 
               
               
                   
                   
                   
                 like protein Type VI secretion system-related 
               
               
                   
                   
                   
                 protein 
               
               
                 209 
                 IPR021459 
                 PF11308 
                 Glycosyl hydrolases related to GH101 family, 
               
               
                   
                   
                   
                 GH129 Glycosyl hydrolases related to GH101 
               
               
                   
                   
                   
                 family, GHL1-GHL3 
               
               
                 210 
                 IPR003610 
                 SM00495 
                 Carbohydrate-binding module family 5/12 
               
               
                 211 
                 IPR036573 
                 SSF51055 
                 Carbohydrate-binding module superfamily 5/12 
               
               
                 212 
                 IPR000184 
                 PF01103 
                 Surface antigen Bacterial surface antigen (D15) 
               
               
                 213 
                 IPR016035 
                 SSF52151 
                 Acyl transferase/acyl 
               
               
                   
                   
                   
                 hydrolase/lysophospholipase 
               
               
                 214 
                 IPR002641 
                 PS51635 
                 Patatin-like phospholipase (PNPLA) domain 
               
               
                   
                   
                   
                 profile. Patatin-like phospholipase domain 
               
               
                 215 
                 IPR036852 
                 SSF52743 
                 Peptidase S8/S53 domain superfamily 
               
               
                 216 
                 IPR030400 
                 PS51695 
                 Sedolisin domain profile. Sedolisin domain 
               
               
                 217 
                 IPR023828 
                 PS00138 
                 Serine proteases, subtilase family, serine active 
               
               
                   
                   
                   
                 site. Peptidase S8, subtilisin, Ser-active site 
               
               
                 218 
                 IPR015366 
                 PF09286 
                 Pro-kumamolisin, activation domain Peptidase 
               
               
                   
                   
                   
                 S53, activation domain 
               
               
                 219 
                 IPR011990 
                 SSF48452 
                 Tetratricopeptide-like helical domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 220 
                 IPR037167 
                 G3DSA:2.60.410.10 
                 D-Ala-D-Ala carboxypeptidase, C-terminal 
               
               
                   
                   
                   
                 domain superfamily 
               
               
                 221 
                 IPR015956 
                 SSF69189 
                 Penicillin-binding protein, C-terminal domain 
               
               
                   
                   
                   
                 superfamily 
               
               
                 222 
                 IPR015201 
                 PD068438 
                 ANTIMICROBIAL PEPTIDE FUNGICIDE 
               
               
                   
                   
                   
                 3D-STRUCTURE DEFENSE SEQUENCING 
               
               
                   
                   
                   
                 DIRECT AMP1 PLANT PRECURSOR 
               
               
                   
                   
                   
                 Antimicrobial protein MiAMP1 
               
               
                 223 
                 IPR023099 
                 G3DSA:3.30.386.10 
                 Glycoside hydrolase, family 46, N-terminal 
               
               
                 224 
                 IPR000400 
                 PS60000 
                 Chitosanases families 46 and 80 active sites 
               
               
                   
                   
                   
                 signature. Glycoside hydrolase, family 46 
               
               
                 225 
                 IPR036908 
                 SSF50685 
                 RlpA-like domain superfamily 
               
               
                 226 
                 IPR010611 
                 PF06725 
                 3D domain 3D domain 
               
               
                 227 
                 IPR000757 
                 PF00722 
                 Glycosyl hydrolases family 16 Glycoside 
               
               
                   
                   
                   
                 hydrolase family 16 
               
               
                 228 
                 IPR005546 
                 PS51208 
                 Autotransporter beta-domain profile. 
               
               
                   
                   
                   
                 Autotransporter beta-domain 
               
               
                 229 
                 IPR017186 
                 PIRSF037375 
                 Lipase, autotransporter EstA 
               
               
                 230 
                 IPR036709 
                 SSF103515 
                 Autotransporter beta-domain superfamily 
               
               
                 231 
                 IPR001087 
                 PF00657 
                 GDSL-like Lipase/Acylhydrolase GDSL 
               
               
                   
                   
                   
                 lipase/esterase 
               
               
                 232 
                 IPR037460 
                 cd01823 
                 SEST like  Streptomyces  scabies esterase-like 
               
               
                 233 
                 IPR008258 
                 PF01464 
                 Transglycosylase SLT domain Transglycosylase 
               
               
                   
                   
                   
                 SLT domain 1 
               
               
                 234 
                 IPR000189 
                 PS00922 
                 Prokaryotic transglycosylases signature. 
               
               
                   
                   
                   
                 Prokaryotic transglycosylase, active site 
               
               
                 235 
                 IPR030873 
                 MF_00997 
                 Beta-barrel assembly-enhancing protease 
               
               
                   
                   
                   
                 [bepA]. Beta-barrel assembly-enhancing 
               
               
                   
                   
                   
                 protease 
               
               
                 236 
                 IPR010964 
                 TIGR01887 
                 dipeptidaselike: putative dipeptidase Peptidase 
               
               
                   
                   
                   
                 M20A, peptidase V-related 
               
               
                 237 
                 IPR012341 
                 G3DSA:1.50.10.10 
                 Six-hairpin glycosidase-like superfamily 
               
               
                 238 
                 IPR008928 
                 SSF48208 
                 Six-hairpin glycosidase superfamily 
               
               
                 239 
                 IPR002037 
                 PR00735 
                 Glycosyl hydrolase family 8 signature Glycoside 
               
               
                   
                   
                   
                 hydrolase, family 8 
               
               
                 240 
                 IPR024077 
                 G3DSA:1.10.1370.10 
                 Neurolysin/Thimet oligopeptidase, domain 2 
               
               
                 241 
                 IPR001567 
                 PF01432 
                 Peptidase family M3 Peptidase M3A/M3B 
               
               
                   
                   
                   
                 catalytic domain 
               
               
                 242 
                 IPR034005 
                 cd06456 
                 M3A_DCP Peptidyl-dipeptidase DCP 
               
               
                 243 
                 IPR036913 
                 SSF160113 
                 YegP-like superfamily 
               
               
                 244 
                 IPR010879 
                 PF07411 
                 Domain of unknown function (DUF1508) 
               
               
                   
                   
                   
                 Domain of unknown function DUF1508 
               
               
                 245 
                 IPR012347 
                 G3DSA:1.20.1260.10 
                 Ferritin-like 
               
               
                 246 
                 IPR002024 
                 cd00907 
                 Bacterioferritin Bacterioferritin 
               
               
                 247 
                 IPR009040 
                 PS50905 
                 Ferritin-like diiron domain profile. Ferritin-like 
               
               
                   
                   
                   
                 diiron domain 
               
               
                 248 
                 IPR009078 
                 SSF47240 
                 Ferritin-like superfamily 
               
               
                 249 
                 IPR008331 
                 PF00210 
                 Ferritin-like domain Ferritin/DPS protein 
               
               
                   
                   
                   
                 domain 
               
               
                 250 
                 IPR011837 
                 TIGR02100 
                 glgX_debranch: glycogen debranching enzyme 
               
               
                   
                   
                   
                 GlgX Glycogen debranching enzyme, GlgX type 
               
               
                 251 
                 IPR004193 
                 PF02922 
                 Carbohydrate-binding module 48 (Isoamylase 
               
               
                   
                   
                   
                 N-terminal domain) Glycoside hydrolase, family 
               
               
                   
                   
                   
                 13, N-terminal 
               
               
                 252 
                 IPR022844 
                 MF_01248 
                 Glycogen debranching enzyme [glgX]. 
               
               
                   
                   
                   
                 Glycogen debranching enzyme, bacterial 
               
               
                   
               
            
           
         
       
     
     Example 14: Cloning of Resistance-Conferring Bacterial Genes for Expression in  E. coli    
     The fungicidal effect of isolated bacterial polypeptides was examined by expressing the polypeptides in  E. coli  and purifying the expressed proteins. For expression in  E. coli , selected genes were synthesized by Genscript. The original bacterial sequences were modified such that the codons were optimized for protein expression in  E. coli  (further details are available at genscript.com/tools/codon-frequency-table), and a 6-Histidine sequence tag was inserted at either the 5′ or the 3′ end. 
     In cases where the original bacterial sequences included a nucleic acid sequence encoding a native signal peptide, it was removed up to the native cleavage site separating the encoded mature protein (i.e., the protein not including the signal peptide) from the native signal peptide. The polynucleotide encoding the mature protein was further modified by adding an artificial initiator Methionine codon immediately after the cleavage site. 
     All optimized genes were synthesized with 5′ NcoI and 3′ EcoRI restrictions sites, and in some of the genes as a result of the addition of the restriction site an additional glycine residue codon was added at the 2 nd  position (after the initiator Methionine codon) in order to maintain the reading frame of the coding sequence. 
     Genes lacking an original (native) signal peptide were cloned into pET22bd (a modified version of pET22B+ in which the periplasmic signal peptide PelB SEQ ID NO:5 was removed). 
     Genes having an original (native) signal peptide that was replaced with an artificial signal peptide were cloned into either the pET22bd and/or the pET22B+(purchased from Merck Millipore, merckmillipore. com/INTL/en/product/pET-22b %28%2B %29-DNA---Novagen,EMD_BIO-69744?ReferrerURL=%3A%2F%2Fwww.google.co(dot)il%2F&amp;bd=1#anchor_Description) by digesting the gene and the vector with NcoI and EcoRI. 
     The sequence of each gene was verified by Sanger sequencing in each expression vector. All aforementioned modifications are summarized in Table 19 below. 
     
       
         
           
               
             
               
                 TABLE 19 
               
             
            
               
                   
               
               
                 Sequences synthesized for cloning in  E. coli   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Native 
                 Derived 
                 Synthesized 
                 Synthesized 
                   
               
               
                 Gene 
                 Polyp. 
                 Polyp. 
                 Polyn. 
                 Polyp. 
               
               
                 Name 
                 SEQ ID NO 
                 SEQ ID NO 
                 SEQ ID NO 
                 SEQ ID NO 
                 Modifications 
               
               
                   
               
               
                 BLFS1 
                 2444 
                 4776 
                 4420 
                 4617 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS2 
                 2445 
                 — 
                 4442 
                 4639 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS3 
                 2446 
                 4698 
                 4316 
                 4513 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS4 
                 2447 
                 — 
                 4392 
                 4589 
                 3′ His-tag added 
               
               
                 BLFS5 
                 2448 
                 4744 
                 4373 
                 4570 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS6 
                 2449 
                 4682 
                 4298 
                 4495 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS7 
                 2450 
                 4753 
                 4386 
                 4583 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS8 
                 2451 
                 4771 
                 4414 
                 4611 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS9 
                 2452 
                 4757 
                 4394 
                 4591 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS10 
                 2453 
                 4690 
                 4306 
                 4503 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS11 
                 2454 
                 4669 
                 4284 
                 4481 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS12 
                 2455 
                 4788 
                 4435 
                 4632 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS13 
                 2456 
                 4673 
                 4288 
                 4485 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS14 
                 2457 
                 4722 
                 4344 
                 4541 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS15 
                 2458 
                 4678 
                 4294 
                 4491 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS16 
                 2459 
                 4681 
                 4297 
                 4494 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS17 
                 2460 
                 4671 
                 4286 
                 4483 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS18 
                 2461 
                 — 
                 4265 
                 4462 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS19 
                 2462 
                 4712 
                 4334 
                 4531 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS20 
                 2463 
                 4774 
                 4417 
                 4614 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS21 
                 2464 
                 4751 
                 4382 
                 4579 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS22 
                 2465 
                 4787 
                 4434 
                 4631 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS23 
                 2466 
                 4653 
                 4259 
                 4456 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS24 
                 2467 
                 — 
                 4395 
                 4592 
                 3′ His-tag added 
               
               
                 BLFS25 
                 2468 
                 4799 
                 4449 
                 4646 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS26 
                 2469 
                 4784 
                 4430 
                 4627 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS29 
                 2471 
                 4657 
                 4263 
                 4460 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS30 
                 2472 
                 — 
                 4408 
                 4605 
                 3′ His-tag added 
               
               
                 BLFS31 
                 2473 
                 4726 
                 4348 
                 4545 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS32 
                 2474 
                 4752 
                 4384 
                 4581 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS33 
                 2475 
                 4701 
                 4319 
                 4516 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS34 
                 2476 
                 4650 
                 4256 
                 4453 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS35 
                 2477 
                 4778 
                 4423 
                 4620 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS36 
                 2478 
                 4772 
                 4415 
                 4612 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS37 
                 2479 
                 — 
                 4368 
                 4565 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS38 
                 2480 
                 — 
                 4281 
                 4478 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS39 
                 2481 
                 4732 
                 4356 
                 4553 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS41 
                 2482 
                 4781 
                 4427 
                 4624 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS42 
                 2483 
                 4763 
                 4404 
                 4601 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS43 
                 2484 
                 4683 
                 4299 
                 4496 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS44 
                 2485 
                 4713 
                 4335 
                 4532 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS45 
                 2486 
                 4745 
                 4374 
                 4571 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS46 
                 2487 
                 4694 
                 4311 
                 4508 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS47 
                 2488 
                 4686 
                 4302 
                 4499 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS48 
                 2489 
                 4699 
                 4317 
                 4514 
                 Native lipid anchor removed; 
               
               
                   
                   
                   
                   
                   
                 added MetGly; 3′ His-tag 
               
               
                 BLFS49 
                 2490 
                 4790 
                 4438 
                 4635 
                 Native lipid anchor removed; 
               
               
                   
                   
                   
                   
                   
                 added MetGly; 3′ His-tag 
               
               
                 BLFS50 
                 2491 
                 4754 
                 4388 
                 4585 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS51 
                 2492 
                 4692 
                 4309 
                 4506 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS52 
                 2493 
                 4737 
                 4364 
                 4561 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS53 
                 2494 
                 — 
                 4433 
                 4630 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS55 
                 2495 
                 4748 
                 4378 
                 4575 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS56 
                 2496 
                 4651 
                 4257 
                 4454 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS57 
                 2497 
                 4738 
                 4365 
                 4562 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS58 
                 2498 
                 4743 
                 4371 
                 4568 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS59 
                 2499 
                 4674 
                 4290 
                 4487 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS60 
                 2500 
                 4730 
                 4353 
                 4550 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS61 
                 2501 
                 — 
                 4325 
                 4522 
                 3′ His-tag added 
               
               
                 BLFS62 
                 2502 
                 4691 
                 4308 
                 4505 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS63 
                 2503 
                 4697 
                 4315 
                 4512 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS64 
                 2504 
                 4654 
                 4260 
                 4457 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS65 
                 2505 
                 4719 
                 4341 
                 4538 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS67 
                 2506 
                 4782 
                 4428 
                 4625 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS68 
                 2507 
                 4696 
                 4314 
                 4511 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS69 
                 2508 
                 4655 
                 4261 
                 4458 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS70 
                 2509 
                 — 
                 4269 
                 4466 
                 3′ His-tag added 
               
               
                 BLFS72 
                 2510 
                 — 
                 4278 
                 4475 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS73 
                 2511 
                 — 
                 4312 
                 4509 
                 3′ His-tag added 
               
               
                 BLFS74 
                 2512 
                 4758 
                 4396 
                 4593 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS75 
                 2513 
                 4688 
                 4304 
                 4501 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS76 
                 2514 
                 4710 
                 4329 
                 4526 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS77 
                 2515 
                 4668 
                 4279 
                 4476 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS79 
                 2516 
                 4665 
                 4275 
                 4472 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS80 
                 2517 
                 4709 
                 4328 
                 4525 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS82 
                 2518 
                 — 
                 4282 
                 4479 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS83 
                 2519 
                 4750 
                 4380 
                 4577 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS84 
                 2520 
                 4663 
                 4273 
                 4470 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS85 
                 2521 
                 4765 
                 4406 
                 4603 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS86 
                 2522 
                 4724 
                 4346 
                 4543 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS87 
                 2523 
                 4648 
                 4254 
                 4451 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS88 
                 2524 
                 4786 
                 4432 
                 4629 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS89 
                 2525 
                 4652 
                 4258 
                 4455 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS90 
                 2526 
                 — 
                 4376 
                 4573 
                 3′ His-tag added 
               
               
                 BLFS91 
                 2527 
                 4703 
                 4321 
                 4518 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS92 
                 2528 
                 4769 
                 4412 
                 4609 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS93 
                 2529 
                 4756 
                 4391 
                 4588 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS94 
                 2530 
                 4687 
                 4303 
                 4500 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS95 
                 2531 
                 4740 
                 4367 
                 4564 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS96 
                 2532 
                 — 
                 4419 
                 4616 
                 3′ His-tag added 
               
               
                 BLFS97 
                 2533 
                 — 
                 4443 
                 4640 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS98 
                 2534 
                 4783 
                 4429 
                 4626 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS99 
                 2535 
                 4729 
                 4352 
                 4549 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS100 
                 2536 
                 4746 
                 4375 
                 4572 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS101 
                 2537 
                 4780 
                 4425 
                 4622 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS102 
                 2538 
                 4725 
                 4347 
                 4544 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS103 
                 2539 
                 — 
                 4331 
                 4528 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS104 
                 2540 
                 4768 
                 4410 
                 4607 
                 Native lipid anchor removed; 
               
               
                   
                   
                   
                   
                   
                 added MetGly; 3′ His-tag 
               
               
                 BLFS105 
                 2541 
                 4739 
                 4366 
                 4563 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS106 
                 2542 
                 — 
                 4411 
                 4608 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS107 
                 2543 
                 4773 
                 4416 
                 4613 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS108 
                 2544 
                 — 
                 4399 
                 4596 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS109 
                 2545 
                 4779 
                 4424 
                 4621 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS110 
                 2546 
                 4702 
                 4320 
                 4517 
                 Native lipid anchor removed; 
               
               
                   
                   
                   
                   
                   
                 added MetGly; 3′ His-tag 
               
               
                 BLFS111 
                 2547 
                 4793 
                 4441 
                 4638 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS112 
                 2548 
                 — 
                 4349 
                 4546 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS113 
                 2549 
                 4672 
                 4287 
                 4484 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS114 
                 2550 
                 4705 
                 4323 
                 4520 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS115 
                 2551 
                 4716 
                 4338 
                 4535 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS116 
                 2552 
                 — 
                 4330 
                 4527 
                 3′ His-tag added 
               
               
                 BLFS118 
                 2553 
                 4767 
                 4409 
                 4606 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS119 
                 2554 
                 4741 
                 4369 
                 4566 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS120 
                 2555 
                 — 
                 4361 
                 4558 
                 3′ His-tag added 
               
               
                 BLFS121 
                 2556 
                 4707 
                 4326 
                 4523 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS122 
                 2557 
                 — 
                 4280 
                 4477 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS123 
                 2558 
                 — 
                 4426 
                 4623 
                 3′ His-tag added 
               
               
                 BLFS124 
                 2559 
                 4675 
                 4291 
                 4488 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS125 
                 2560 
                 4723 
                 4345 
                 4542 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS126 
                 2561 
                 4795 
                 4445 
                 4642 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS127 
                 2562 
                 4695 
                 4313 
                 4510 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS128 
                 2563 
                 — 
                 4332 
                 4529 
                 3′ His-tag added 
               
               
                 BLFS129 
                 2564 
                 — 
                 4383 
                 4580 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS130 
                 2565 
                 — 
                 4268 
                 4465 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS131 
                 2566 
                 4693 
                 4310 
                 4507 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS132 
                 2567 
                 4689 
                 4305 
                 4502 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS133 
                 2568 
                 4798 
                 4448 
                 4645 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS134 
                 2569 
                 4679 
                 4295 
                 4492 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS135 
                 2570 
                 4717 
                 4339 
                 4536 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS136 
                 2571 
                 4662 
                 4272 
                 4469 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS137 
                 2572 
                 4728 
                 4351 
                 4548 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS138 
                 2573 
                 — 
                 4385 
                 4582 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS139 
                 2574 
                 4721 
                 4343 
                 4540 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS140 
                 2575 
                 4761 
                 4402 
                 4599 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS141 
                 2576 
                 4785 
                 4431 
                 4628 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS142 
                 2577 
                 4755 
                 4390 
                 4587 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS143 
                 2578 
                 4770 
                 4413 
                 4610 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS144 
                 2579 
                 4718 
                 4340 
                 4537 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS145 
                 2580 
                 4715 
                 4337 
                 4534 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS146 
                 2581 
                 4797 
                 4447 
                 4644 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS147 
                 2582 
                 4764 
                 4405 
                 4602 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS148 
                 2583 
                 4711 
                 4333 
                 4530 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS149 
                 2584 
                 4789 
                 4437 
                 4634 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS150 
                 2585 
                 — 
                 4421 
                 4618 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS151 
                 2586 
                 4664 
                 4274 
                 4471 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS152 
                 2587 
                 4791 
                 4439 
                 4636 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS153 
                 2588 
                 4734 
                 4359 
                 4556 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 BLFS154 
                 2589 
                 4792 
                 4440 
                 4637 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS155 
                 2590 
                 4659 
                 4266 
                 4463 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS156 
                 2591 
                 4660 
                 4267 
                 4464 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS157 
                 2592 
                 4747 
                 4377 
                 4574 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 BLFS158 
                 2593 
                 — 
                 4389 
                 4586 
                 Gly &amp; 3′ His-tag added 
               
               
                 BLFS159 
                 2594 
                 — 
                 4289 
                 4486 
                 Gly &amp; 3′ His-tag added 
               
               
                 PUB6 
                 2595 
                 — 
                 4372 
                 4569 
                 3′ His-tag added 
               
               
                 PUB23 
                 2596 
                 — 
                 4355 
                 4552 
                 Gly &amp; 3′ His-tag added 
               
               
                 PUB31 
                 2597 
                 — 
                 4398 
                 4595 
                 3′ His-tag added 
               
               
                 PUB65 
                 2598 
                 4775 
                 4418 
                 4615 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS1 
                 2599 
                 — 
                 4381 
                 4578 
                 3′ His-tag added 
               
               
                 ZLFS2 
                 2600 
                 4667 
                 4277 
                 4474 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS3 
                 2601 
                 4649 
                 4255 
                 4452 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS5 
                 2602 
                 4647 
                 4253 
                 4450 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS6 
                 2603 
                 — 
                 4283 
                 4480 
                 Gly &amp; 3′ His-tag added 
               
               
                 ZLFS7 
                 2604 
                 4700 
                 4318 
                 4515 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS8 
                 2605 
                 4777 
                 4422 
                 4619 
                 Native lipid anchor removed; 
               
               
                   
                   
                   
                   
                   
                 added MetGly; 3′ His-tag 
               
               
                 ZLFS9 
                 2606 
                 4794 
                 4444 
                 4641 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 ZLFS10 
                 2607 
                 4766 
                 4407 
                 4604 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS11 
                 2608 
                 4677 
                 4293 
                 4490 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS12 
                 2609 
                 — 
                 4360 
                 4557 
                 Gly &amp; 3′ His-tag added 
               
               
                 ZLFS13 
                 2610 
                 4706 
                 4324 
                 4521 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS14 
                 2611 
                 — 
                 4436 
                 4633 
                 Gly &amp; 3′ His-tag added 
               
               
                 ZLFS15 
                 2612 
                 4708 
                 4327 
                 4524 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS16 
                 2613 
                 4714 
                 4336 
                 4533 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 ZLFS17 
                 2614 
                 4731 
                 4354 
                 4551 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 ZLFS18 
                 2615 
                 — 
                 4387 
                 4584 
                 3′ His-tag added 
               
               
                 ZLFS19 
                 2616 
                 4658 
                 4264 
                 4461 
                 Native lipid anchor removed; 
               
               
                   
                   
                   
                   
                   
                 added MetGly; 3′ His-tag 
               
               
                 ZLFS21 
                 2617 
                 4670 
                 4285 
                 4482 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS22 
                 2618 
                 4685 
                 4301 
                 4498 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS23 
                 2619 
                 4759 
                 4397 
                 4594 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS24 
                 2620 
                 4676 
                 4292 
                 4489 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 5&#39; His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 ZLFS25 
                 2621 
                 4680 
                 4296 
                 4493 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS27 
                 2622 
                 4760 
                 4401 
                 4598 
                 Native lipid anchor removed; 
               
               
                   
                   
                   
                   
                   
                 added MetGly; 3′ His-tag 
               
               
                 ZLFS28 
                 2623 
                 4656 
                 4262 
                 4459 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS29 
                 2624 
                 4733 
                 4358 
                 4555 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS30 
                 2625 
                 4666 
                 4276 
                 4473 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 ZLFS31 
                 2626 
                 4704 
                 4322 
                 4519 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 5&#39; His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 ZLFS32 
                 2627 
                 4727 
                 4350 
                 4547 
                 Native lipid anchor removed; 
               
               
                   
                   
                   
                   
                   
                 added Met; 3′ His-tag 
               
               
                 ZLFS33 
                 2628 
                 4742 
                 4370 
                 4567 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS34 
                 2629 
                 4796 
                 4446 
                 4643 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS35 
                 2630 
                 4749 
                 4379 
                 4576 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS36 
                 2631 
                 — 
                 4400 
                 4597 
                 Gly &amp; 3′ His-tag added 
               
               
                 ZLFS37 
                 2632 
                 — 
                 4307 
                 4504 
                 3′ His-tag added 
               
               
                 ZLFS38 
                 2633 
                 — 
                 4393 
                 4590 
                 Gly &amp; 3′ His-tag added 
               
               
                 ZLFS39 
                 2634 
                 — 
                 4357 
                 4554 
                 Gly &amp; 3′ His-tag added 
               
               
                 ZLFS42 
                 2635 
                 4661 
                 4271 
                 4468 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 ZLFS44 
                 2636 
                 4736 
                 4363 
                 4560 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS45 
                 2637 
                 4735 
                 4362 
                 4559 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 ZLFS47 
                 2638 
                 4684 
                 4300 
                 4497 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 ZLFS48 
                 2639 
                 4762 
                 4403 
                 4600 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; MetGly &amp; 3′ His- 
               
               
                   
                   
                   
                   
                   
                 tag added 
               
               
                 ZLFS49 
                 2640 
                 4720 
                 4342 
                 4539 
                 Native signal peptide 
               
               
                   
                   
                   
                   
                   
                 removed; Met &amp; 3′ His-tag 
               
               
                   
                   
                   
                   
                   
                 added 
               
               
                 ZLFS51 
                 2641 
                 — 
                 4270 
                 4467 
                 3′ His-tag added 
               
               
                   
               
               
                 “Polyp.”—Polypeptide. “Polyn.”—Polynucleotide 
               
            
           
         
       
     
     In most cases, following the optimization, the synthesized sequences exhibit at least 70% global identity to the curated sequences from which they were obtained. In several cases replacing the native signal peptide resulted in a polynucleotide and/or polypeptide having less than 70% global identity to their parent sequence. For the genes presented in Table 20, the optimized synthesized polypeptide sequences exhibit less than 70% global identity to the curated sequences from which they were obtained, due to a replacement of relatively long signal sequence. For example, the polypeptide of SEQ ID NO:4502 exhibits only 48.65% global identity to SEQ ID NO:2567 which is the original curated sequence of BLFS132. These polypeptides, however, comprise the core amino acid sequence of the parent polypeptides. 
     
       
         
           
               
             
               
                 TABLE 20 
               
             
            
               
                   
               
               
                 Optimized polypeptides having less that 70% global 
               
               
                 identity to the parent not optimized polypeptide 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Native Polypeptide 
                 Synthesized Polypeptide 
               
               
                   
                 Gene Name 
                 SEQ ID NO. 
                 SEQ ID NO. 
               
               
                   
                   
               
               
                   
                 ZLFS42 
                 2635 
                 4468 
               
               
                   
                 ZLFS47 
                 2638 
                 4497 
               
               
                   
                 BLFS132 
                 2567 
                 4502 
               
               
                   
                 BLFS10 
                 2453 
                 4503 
               
               
                   
                 BLFS86 
                 2522 
                 4543 
               
               
                   
                 BLFS102 
                 2538 
                 4544 
               
               
                   
                 BLFS52 
                 2493 
                 4561 
               
               
                   
                 BLFS119 
                 2554 
                 4566 
               
               
                   
                 BLFS9 
                 2452 
                 4591 
               
               
                   
                 BLFS36 
                 2478 
                 4612 
               
               
                   
                 BLFS20 
                 2463 
                 4614 
               
               
                   
                 BLFS133 
                 2568 
                 4645 
               
               
                   
                   
               
            
           
         
       
     
     Example 15: Purification of Proteins Expressed in Bacterial Cells 
     Transformation of Bacterial Cells with the Polynucleotides Encoding the Polypeptides Having the Ability to Kill or Inhibit the Development of  Fusarium    
     Genes encoding unknown toxin candidate proteins of the present invention were cloned in pET22/T7-lac promoter-based vector and coding DNA sequence was confirmed by sequencing. pET-based expression vectors were transformed into BL21(DE3)  E. coli  host using heat shock method. After overnight growth in Terrific Broth (TB) medium at 37° C. in the presence of Ampicillin (100 μg/mL), 5 mL starter cultures were used to inoculate 100 mL TB culture at OD 600  0.03 in 1 L flat bottom flask. The cultures were allowed to grow until OD 600 ˜0.5 (2-3 hours at 37° C. with 250 rpm). The incubator shaker temperature was reduced to 22° C. and Isopropyl β-D-1-thiogalactopyranoside (IPTG) was added at a final concentration of 1 mM. The cultures were incubated further for 15 to 18 hours for target protein expression and cells were harvested by centrifuging at 4,000 rpm/4° C./10 minutes. The cell pellet was washed with cold water and stored at −80° C. until used for protein purification. 
     Bacterial cell pellet was lysed using B-PER™ Bacterial Protein Extraction Reagent (Thermo Fisher Scientific, catalog number: 78248) at room temperature for 1 hour. The supernatant fraction (containing soluble protein) was clarified by centrifugation at 16000 rcf/4° C./20 minutes. 
     Purification of Expressed Recombinant Polypeptides: 
     Soluble fractions—The supernatant fraction containing soluble protein was incubated with Ni-NTA beads (washed with binding buffer prior to addition of supernatant fraction: 50 mM potassium phosphate pH 8.0, 600 mM NaCl and 20 mM imidazole) for 1 hour at 4° C. on a rotatory shaker with gentle shaker speed. The Ni-NTA-protein bound beads were collected by centrifugation at 800 rcf/4° C./3 minutes. The Ni-NTA-protein bound beads were washed with washing buffer (50 mM potassium phosphate pH 8.0, 600 mM NaCl, 0.5% Triton and 20 mM imidazole) for 3 times. The bound proteins were eluted with elution buffer (50 mM potassium phosphate pH 8.0, 600 mM NaCl and 250 mM imidazole). The salts in the eluted proteins were removed by dialysis. Samples were loaded on a Pur-A+Lyzer tubes (catalog number: PurR35100) and placed in a beaker containing 0.5 liter of sterile dialysis buffer (16.5 mM Potassium phosphate buffer pH 5.0) for 1 night/weekend in the refrigerator at 8° C. SDS-PAGE analysis was used to quantify protein using known concentrations of bovine serum albumin (BSA) as standard. The known concentrations of toxin candidates were used for bioassay. 
     Example 16: Effect of Isolated Proteins on Fungal Growth 
     The spore assay is an in vitro bioassay in a 96 wells plate format, implementing spores from a  Fusarium verticillioides  (Fv) GFP-labeled strain and the candidate protein purified from bacterial cells as described hereinabove. Mycelia growth of fungi treated with the protein was compared to the growth of untreated fungi and growth inhibition was quantified by detection of GFP fluorescence (475 nm excitation, 509 nm emission). Optical Density (OD) of 620 nm is also measured for QA purposes. 
     Fresh spores were prepared, quantified and dispensed in each well (500 spores) in 50 μl 2×PDB and incubated for 2-3 hours at 25° C. before protein addition. 
     Purified clean proteins prepared as described hereinabove were quantified and diluted with buffer phosphate pH 5.0 at the desired concentration and added to each assay well (up to 40 μM) for a final reaction volume of 100 μl. The plates are incubated for 72 hr at 25° C. and responses were measured every 24 hr. 
     Each protein treatment in each plate was compared to the corresponding spore growth in untreated control by t-test α=0.05 one tailed 
     Table 21 summarizes the results of two independent assays using purified proteins from bacterial cells over-expressing the polypeptides of some embodiments of the invention. 
     
       
         
           
               
             
               
                 TABLE 21 
               
             
            
               
                   
               
               
                 Inhibition of  Fusarium  mycelia growth by proteins 
               
               
                 of the invention isolated from bacteria 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 First assay 
                 Second assay 
               
               
                 Gene Name 
                 General annotation 
                 % Inhibition ** 
                 % Inhibition ** 
               
               
                   
               
               
                 ZLFS42 
                 Killer toxin like 
                 90% (72 hr) 
                 86% (72 hr) 
               
               
                   
                   
                 20 μM 
                 25 μM 
               
               
                 ZLFS48 
                 Antimicrobial protein MiAMP1 
                 56% (48 hr) 
                 30% (72 hr) 
               
               
                   
                   
                 25 μM 
                 20 μM 
               
               
                 BLFS129 
                 Alginate lyase 
                 85% (48 hr) 
                 69% (72 hr) 
               
               
                   
                   
                 6.4 μM 
                 4 μM 
               
               
                 BLFS139 
                 Peptidoglycan endopeptidase 
                 72% (48 hr) 
                 64% (48 hr) 
               
               
                   
                   
                 14 μM 
                 6 μM 
               
               
                 BLFS128 
                 Peptidoglycan hydrolase flgj 
                 37% (48 hr) 
                 52% (48 hr) 
               
               
                   
                   
                 14 μM 
                 3 μM 
               
               
                 BLFS70 
                 Hypothetical protein 
                 36% (48 hr) 
                 52% (48 hr) 
               
               
                   
                   
                 2 μM 
                 3 μM 
               
               
                 BLFS111 
                 Hypothetical protein) 
                 20% (72 hr) 
                 63% (48 hr) 
               
               
                   
                   
                 4.5 μM 
                 10 μM 
               
               
                 BLFS157 
                 Hypothetical protein 
                 37% (48 hr) 
                 41% (48 hr) 
               
               
                   
                   
                 12 μM 
                 10 μM 
               
               
                 BLFS101 
                 Metalloprotease loiP 
                 47% (24 hr) 
                 70% (48 hr) 
               
               
                   
                   
                 25 μM 
                 25 ␣M 
               
               
                 BLFS63 
                 n-acetylmuramoyl-1-alanine 
                 44% (48 hr) 
                 55% (48 hr) 
               
               
                   
                 amidase amib 
                 39 μM 
                 2 μM 
               
               
                 ZLFS1 
                 Arthropod defensin 
                 29% (48 hr) 
                 32% (48 hr) 
               
               
                   
                   
                 13 μM 
                 27 μM 
               
               
                 BLFS155 
                 Mannose-binding protein 
                 45% (48 hr) 
                 70% (48 hr) 
               
               
                   
                   
                 25 μM 
                 44 μM 
               
               
                 BLFS7 
                 Peptidase C11 clostripain 
                 65% (48 hr) 
                 36% (48 hr) 
               
               
                   
                   
                 &lt;1 μM 
                 5 μM 
               
               
                 BLFS140 
                 Uncharacterized lipoprotein ybbd 
                 75% (48 hr) 
                 63% (48 hr) 
               
               
                   
                   
                 9 μM 
                 40 μM 
               
               
                 BLFS103 
                 Toxin tccc3 
                 67% (48 hr) 
                 56% (48 hr) 
               
               
                   
                   
                 3 μM 
                 3.75 μM 
               
               
                 BLFS46 
                 Lysm domain protein 
                 46% (48 hr) 
                 32% (48 hr) 
               
               
                   
                   
                 18 μM 
                 40 μM 
               
               
                 BLFS115 
                 Probable periplasmic serine 
                 39% (48 hr) 
                 39% (48 hr) 
               
               
                   
                 endoprotease DegP-like 
                 23 μM 
                 40 μM 
               
               
                 BLFS47 
                 Hypothetical protein 
                 11% (72 hr) 
                 25% (48 hr) 
               
               
                   
                   
                 40 μM 
                 0.5 μM 
               
               
                 BLFS87 
                 Endoglucanase b 
                 25% (48 hr) 
                 27% (48 hr) 
               
               
                   
                   
                 40 μM 
                 40 μM 
               
               
                 BLFS102 
                 Hypothetical protein 
                 30% (48 hr) 
                 44% (48 hr) 
               
               
                   
                   
                 40 μM 
                 40 μM 
               
               
                 BLFS42 
                 Chitin-binding protein 
                 21% (72 hr) 
                 60% (48 hr) 
               
               
                   
                   
                 25 μM 
                 40 μM 
               
               
                 ZLFS29 
                 Beta-barrel assembly-enhancing 
                 36.5% (48 hr) 
                 44% (48 hr) 
               
               
                   
                 protease [bepA]. 
                 40 μM 
                 40 μM 
               
               
                 BLFS154 
                 Peptidase s1 
                 52% (48 hr) 
                 33% (48 hr) 
               
               
                   
                   
                 40 μM 
                 40 μM 
               
               
                 BLFS62 
                 Murein hydrolase activator nlpd 
                 53% (24 hr) 
                 27% (48 hr) 
               
               
                   
                   
                 40 μM 
                 40 μM 
               
               
                 BLFS19 
                 Peptidase p60 
                 31% (48 hr) 
                 29% (48 hr) 
               
               
                   
                   
                 40 μM 
                 40 μM 
               
               
                 ZLFS16 
                 d-alanyl-d-alanine 
                 11% (24 hr) 
                 36% (24 hr) 
               
               
                   
                 carboxypeptidase dacd 
                 41 μM 
                 38 μM 
               
               
                 ZLFS19 
                 Lipoprotein nlpd/lppb homolog 
                 21% (48 hr) 
                 33% (24 hr) 
               
               
                   
                   
                 38 μM 
                 37 μM 
               
               
                 BLFS1 
                 Antifungal protein 
                 57% (48 hr) 
                 31% (48 hr) 
               
               
                   
                   
                 40 μM 
                 40 μM 
               
               
                 BLFS9 
                 Oxidoreductase 
                 21% (48 hr) 
                 15% (48 hr) 
               
               
                   
                   
                 25 μM 
                 18 μM 
               
               
                 BLFS80 
                 Hypothetical protein 
                 33% (48 hr) 
                 43% (48 hr) 
               
               
                   
                   
                 40 μM 
                 40 μM 
               
               
                 BLFS123 
                 Peptidase c1 
                 17% (72 hr) 
                 1% (48 hr) 
               
               
                   
                   
                 20 μM 
                 20 μM 
               
               
                 BLFS113 
                 Transpeptidase 
                 11% (72 hr) 
                 19% (48 hr) 
               
               
                   
                   
                 40 μM 
                 25 μM 
               
               
                 BLFS121 
                 Glutamate carboxypeptidase 
                 26% (48 hr) 
                 14% (24 hr) 
               
               
                   
                   
                 40 μM 
                 25 μM 
               
               
                 BLFS142 
                 Uncharacterized protein ykvt 
                 34% (48 hr) 
                 31% (48 hr) 
               
               
                   
                   
                 21 μM 
                 42 μM 
               
               
                 ZLFS5 
                 Hypothetical protein 
                 29% (48 hr) 
                 15% (48 hr) 
               
               
                   
                   
                 20 μM 
                 40 μM 
               
               
                 ZLFS32 
                 Endoglucanase 
                 29% (48 hr) 
                 16% (48 hr) 
               
               
                   
                   
                 20 μM 
                 40 μM 
               
               
                 ZLFS22 
                 Hypothetical protein 
                 95% (72 hr) 
                 11% (24 hr) 
               
               
                   
                   
                 40 μM 
                 40 μM 
               
               
                 ZLFS51 
                 Glycosyl hydrolase 
                 36% (24 hr) 
                 3% (24 hr) 
               
               
                   
                   
                 40 μM 
                 40 μM 
               
               
                 ZLFS27 
                 Lytic transglycosylase 
                 28% (48 hr) 
                 87% (48 hr) 
               
               
                   
                   
                 40 μM 
                 20 μM 
               
               
                 ZLFS47 
                 Antimicrobial protein MiAMP1 
                 5% (48 hr) 
                 56% (48 hr) 
               
               
                   
                   
                 20 μM 
                 25 μM 
               
               
                 ZLFS45 
                 Oxidoreductase 
                 63% (24 hr) 
                 81% (72 hr) 
               
               
                   
                   
                 20 μM 
                 40 μM 
               
               
                   
               
               
                 ** = Best inhibition value scored at defined time and concentration; α = 0.05 and % inhibition &gt; 10% 
               
            
           
         
       
     
     The foregoing description of the specific embodiments will so fully reveal the general nature of the invention that others can, by applying current knowledge, readily modify and/or adapt for various applications such specific embodiments without undue experimentation and without departing from the generic concept, and, therefore, such adaptations and modifications should and are intended to be comprehended within the meaning and range of equivalents of the disclosed embodiments. It is to be understood that the phraseology or terminology employed herein is for the purpose of description and not of limitation. The means, materials, and steps for carrying out various disclosed functions may take a variety of alternative forms without departing from the invention.