Patent Publication Number: US-2023139282-A1

Title: Antimalarial agents

Description:
FIELD OF THE INVENTION 
     The present invention relates to compounds of Formula (I), or pharmaceutically acceptable salts thereof, useful for the treatment of  Plasmodium  infections. More specifically, the present invention relates to compounds of Formula (I), or pharmaceutically acceptable salts thereof, useful for the treatment of  Plasmodium  infections, more particularly for the treatment of malaria. 
     BACKGROUND OF THE INVENTION 
     Malaria is a major disease in humans with several hundred million infections and over 450,000 deaths each year. The most lethal form of malaria is caused by  Plasmodium falciparum . This protozoan parasite is responsible for almost all malarial deaths with most occurring in Africa.  P. falciparum  has a complex life cycle starting in the  Anopheles  mosquito vector when sporozoite forms are injected into the human host during a blood feed. These sporozoites migrate to the liver and invade hepatocytes in which they develop to form thousands of liver merozoites that egress into the blood where they invade erythrocytes to commence the asexual cycle of the parasite responsible for the symptoms of malaria. The parasite develops within the protected niche of the red cell to form 16-32 merozoites that, once mature, egress from the host cell to invade new red blood cells. Some of these parasites differentiate to form gametocytes, the sexual form of the parasite. These can be taken up by the mosquito where male and female gametes form, fuse and differentiate into oocysts on the mosquito midgut extracellular matrix. Sporozoites form within the oocyst and upon egress migrate to the salivary gland for delivery to the next host during blood feeding for perpetuation and survival of the parasite. 
     Other forms of malaria include a relapsing form of malaria caused by  P. vivax  which is responsible for significant morbidity, can cause virulent forms of this disease with some deaths and is mainly a problem outside Africa.  P. knowlesi  is found in South East Asia and is a zoonotic parasite that normally infects long-tailed macaques but has been shown to infect humans in Malaysian Borneo. 
     Artemisinin combined with partner drugs have become a mainstay in the treatment and control of malaria. However, due to the increasing threat of artemisinin-based combination therapy (ACT) drug resistance, the development of new antimalarials with novel targets that inhibit multiple steps in the parasite life cycle is an urgent priority for the malaria control field. Such novel antimalarials, as monotherapies or ACT partner drugs, could make strides towards malaria elimination as there is a reduced likelihood of parasites with preexisting resistance mutations being present in the parasite population. 
     Currently, aspartic acid proteases are prime targets for drug development: the HIV aspartic acid protease has been successfully targeted with a drug in clinical use; inhibitors that target human renin, BACE1 and gamma-secretase have been or are in clinical development. In the antimalarial drug space,  P. falciparum  aspartic acid proteases plasmepsin X and IX (PMX and PMIX) have been identified as potential targets since inhibitors block parasite egress and invasion of the host cell and prevent maturation of some rhoptry and micronemal proteins required for this process (Pino P, Caldelari R, Mukherjee B, Vahokoski J, Klages N, Maco B, et al. A multistage antimalarial targets the plasmepsins IX and X essential for invasion and egress. Science. 2017; 358(6362):522-8.) 
     SUMMARY OF THE INVENTION 
     The present invention is directed to compounds of Formula (I): 
     
       
         
         
             
             
         
       
     
     wherein are described below. 
     Also described herein are methods of treatment of  Plasmodium  infections comprising administering to a subject in need thereof a compound of Formula (I), or a pharmaceutically acceptable salt thereof. Also described herein are methods of treatment of  Plasmodium  infections comprising administering to a subject in need thereof a compound of Formula (I), or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier. 
     Also described herein are methods of treatment of malaria comprising administering to a subject in need thereof a compound of Formula (I), or a pharmaceutically acceptable salt thereof. 
     The present invention further provides the use of compositions, including pharmaceutical compositions, comprising one or more compounds of the invention (e.g., one compound of the invention), or a tautomer thereof, or a pharmaceutically acceptable salt or solvate of said compound(s) and/or said tautomer(s), optionally together with one or more additional therapeutic agents, optionally in an acceptable (e.g., pharmaceutically acceptable) carrier or diluent, for the treatment of malaria. 
     Moreover, the present invention provides methods for the use of pharmaceutical compositions comprising one or more of said compounds in the free form or in pharmaceutically acceptable salt form, together with one or more customary pharmaceutical excipient(s), for the treatment of  Plasmodium  infections, the treatment of malaria, the inhibition of plasmepsin X, or the dual inhibition of plasmepsin X and plasmepsin IX. Methods for the use of combinations of the compounds or salts of the invention together with one or more additional pharmaceutically active agents are also provided. 
     The present invention further provides methods for the inhibition of plasmepsin X, or the dual inhibition of plasmepsin X and plasmepsin IX activity and of treatment, prevention, amelioration and/or delaying onset of diseases or disorders in which the inhibition of plasmepsin X and/or plasmepsin IX has or may have a therapeutic effect, e.g. malaria. 
     The present invention further provides methods for the inhibition of  P. falciparum  aspartic acid proteases. The present invention further provides methods for blocking  P. falciparum  growth by inhibiting plasmepsin X. The present invention further provides methods for blocking  P. falciparum  growth by inhibiting both PMX and Plasmepsin IX. 
     The present invention further provides methods for the treatment of malaria by inhibiting plasmepsin X. The present invention further provides methods for the treatment of malaria by inhibiting both PMX and Plasmepsin IX. 
     These and other embodiments of the invention, which are described in detail below or will become clear to those of ordinary skill in the art, are included within the scope of the invention. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Described herein are compounds having the structural Formula (I): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein:
 
U is N or CH, wherein when U is N, X, Y and Z are CH;
 
X is N or CH, wherein when X is N, U, Y and Z are CH;
 
Y is N or CH, wherein when Y is N, X, U and Z are CH;
 
Z is N or CH, wherein when Z is N, X, Y and U are CH;
 
R 1  is a heterocycloalkyl or C 3 -C 12 cycloalkyl, wherein the heterocycloalkyl or C 3 -C 12 cycloalkyl is unsubstituted or substituted with 1 to 5 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6  alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, oxo, COOC 1 -C 6 alkyl, phenyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) and C 1 -C 6 alkylN(R 7 )(R 8 );
 
R 2  is hydrogen, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl or C 1 -C 6 alkylOH;
 
R 3  is halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) or C 1 -C 6 alkylN(R 7 )(R 8 );
 
R 4  is hydrogen, halogen, CN, C 1 -C 6 alkylCN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylOhaloC 1 -C 6 alkyl, C 1 -C 6 alkylOC 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, C 1 -C 6 alkylSO 2 C 1 -C 6 alkyl, C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, C 1 -C 6 alkenyl, C 1 -C 6 alkynyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ), C 1 -C 6 alkyl(OCH 2 CH 2 ) m N 3 , or C 1 -C 6 alkylN(R 7 )(R 8 ), wherein the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl, C 1 -C 6 alkylOH, C 1 -C 6 alkylOC 1 -C 6 alkyl or C 3 -C 6 cycloalkyl is unsubstituted or substituted with 1 to 3 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, oxo, COOC 1 -C 6 alkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, SO 2 C 1 -C 6 alkyl, C 1 -C 6 alkySO 2 C 1 -C 6 alkyl, OSO 2 F, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) and C 1 -C 6 alkylN(R 7 )(R 8 );
 
R 5  is hydrogen, halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, phenyl, C 1 -C 6 alkylC 3 -C 6 cycloalkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) or C 1 -C 6 alkylN(R 7 )(R 8 ) or when taken with R 6  forms a C 3 -C 6 cycloalkyl or C 3 -C 6 heterocycloalkyl or when R 5  is C 1 -C 6 alkyl and R 1  is a heterocycloalkyl or C 3 -C 12 cycloalkyl, R 5  optionally bonds to R 1  to form a macrocycle;
 
R 6  is hydrogen, halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, phenyl, C 1 -C 6 alkylC 3 -C 6 cycloalkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) or C 1 -C 6 alkylN(R 7 )(R 8 ) or when taken with R 5  forms a C 3 -C 6 cycloalkyl or C 3 -C 6 heterocycloalkyl;
 
R 7  is hydrogen, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl or C 1 -C 6 alkylOH;
 
R 8  is hydrogen, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl or C 1 -C 6 alkylOH;
 
R 9  is hydrogen, halogen, or C 1 -C 6 alkyl;
 
m is 1, 2 or 3; and
 
n is 0, 1, 2 or 3.
 
     Also, described herein are compounds having the structural Formula (I): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein:
 
U is N or CH, wherein when U is N, X, Y and Z are CH;
 
X is N or CH, wherein when X is N, U, Y and Z are CH;
 
Y is N or CH, wherein when Y is N, X, U and Z are CH;
 
Z is N or CH, wherein when Z is N, X, Y and U are CH;
 
R 1  is a heterocycloalkyl or C 3 -C 12 cycloalkyl, wherein the heterocycloalkyl or C 3 -C 12 cycloalkyl is unsubstituted or substituted with 1 to 5 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, oxo, COOC 1 -C 6 alkyl, phenyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) and C 1 -C 6 alkylN(R 7 )(R 8 );
 
R 2  is hydrogen, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl or C 1 -C 6 alkylOH;
 
R 3  is halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) or C 1 -C 6 alkylN(R 7 )(R 8 );
 
R 4  is hydrogen, halogen, CN, C 1 -C 6 alkylCN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylOhaloC 1 -C 6 alkyl, C 1 -C 6 alkylOC 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, C 1 -C 6 alkylSO 2 C 1 -C 6 alkyl, C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, C 1 -C 6 alkenyl, C 1 -C 6 alkynyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ), C 1 -C 6 alkyl(OCH 2 CH 2 ) m N 3 , or C 1 -C 6 alkylN(R 7 )(R 8 ), wherein the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is unsubstituted or substituted with 1 to 3 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, oxo, COOC 1 -C 6 alkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, SO 2 C 1 -C 6 alkyl, C 1 -C 6 alkySO 2 C 1 -C 6 alkyl, OSO 2 F, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) and C 1 -C 6 alkylN(R 7 )(R 8 );
 
R 5  is hydrogen, halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, phenyl, C 1 -C 6 alkylC 3 -C 6 cycloalkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) or C 1 -C 6 alkylN(R 7 )(R 8 ) or when taken with R 6  forms a C 3 -C 6 cycloalkyl or C 3 -C 6 heterocycloalkyl or when R 5  is C 1 -C 6 alkyl and R 1  is a heterocycloalkyl or C 3 -C 12 cycloalkyl, R 5  optionally bonds to R 1  to form a macrocycle;
 
R 6  is hydrogen, halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, phenyl, C 1 -C 6 alkylC 3 -C 6 cycloalkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) or C 1 -C 6 alkylN(R 7 )(R 8 ) or when taken with R 5  forms a C 3 -C 6 cycloalkyl or C 3 -C 6 heterocycloalkyl;
 
R 7  is hydrogen, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl or C 1 -C 6 alkylOH;
 
R 8  is hydrogen, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl or C 1 -C 6 alkylOH;
 
R 9  is hydrogen, halogen, or C 1 -C 6 alkyl;
 
m is 1, 2 or 3; and
 
n is 0, 1, 2 or 3.
 
     In certain embodiments described herein, U is N or CH. In certain embodiments, U is N. In certain embodiments, U is CH. In certain embodiments, U is N, and X, Y and Z are CH. In certain embodiments described herein, X is N or CH. In certain embodiments, X is N. In certain embodiments, X is CH. In certain embodiments, X is N, and U, Y and Z are CH. In certain embodiments described herein, Y is N or CH. In certain embodiments, Y is N. In certain embodiments, Y is CH. In certain embodiments, Y is N, and X, U and Z are CH. In certain embodiments described herein, Z is N or CH. In certain embodiments, Z is N. In certain embodiments, Z is CH. In certain embodiments, Z is N, and X, Y and U are CH. In certain embodiments, each of U, Z, Y and Z is CH. 
     With regard to the compounds described herein, R 1  is a heterocycloalkyl or C 3 -C 12 cycloalkyl, wherein the heterocycloalkyl or C 3 -C 12 cycloalkyl is unsubstituted or substituted with 1 to 5 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, oxo, COOC 1 -C 6 alkyl, phenyl, C 3 -C 6 cycloalkyl, spiroC 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) and C 1 -C 6 alkylN(R 7 )(R 8 ). 
     In certain embodiments, R 1  is a bicyclic ring. In certain embodiments, R 1  is a bicyclic heterocycloalkyl or bicyclic cycloalkyl. 
     In certain embodiments, R 1  is a heterocycloalkyl. Non-limiting examples of monocyclic heterocycloalkyl groups include piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof. Non-limiting examples of bicyclic heterocycloalkyl groups include, but are not limited to, or. 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 1  is a C 3 -C 12 cycloalkyl. In certain embodiments, the cycloalkyl is a monocyclic cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, the cycloalkyl is a bicyclic cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to: 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 1  is unsubstituted. In other embodiments, R 1  is substituted with 1 to 5 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, oxo, COOC 1 -C 6 alkyl, phenyl, C 3 -C 6 cycloalkyl, spiroC 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) and C 1 -C 6 alkylN(R 7 )(R 8 ). In certain embodiments, R 1  is substituted with 1 substituent. 
     In certain embodiments, R 1  is substituted with 2 substituents. In certain embodiments, R 1  is substituted with 3 substituents. In certain embodiments, R 1  is substituted with 4 substituents. In certain embodiments, R 1  is substituted with 5 substituents. In other embodiments, R 1  is unsubstituted or substituted with 1 to 3 substituents. 
     In certain embodiments, R 1  is substituted with halogen. Examples of suitable halogens include, but are not limited to, chlorine, bromine, fluorine and iodine. In certain embodiments, R 1  is substituted with CN. In certain embodiments, R 1  is substituted with OH. In certain embodiments, R 1  is substituted with an oxo group. In certain embodiments, R 1  is substituted with alkoxy. Suitable alkoxys include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. In certain embodiments, R 1  is substituted with C 1 -C 6 alkylOC 1 -C 6 alkyl. In certain embodiments, R 1  is substituted with C 1 -C 6 alkylCOOH. In certain embodiments, R 1  is substituted with COOH. In certain embodiments, R 1  is substituted with C 1 -C 6 alkylCOOC 1 -C 6 alkyl. In certain embodiments R 1  is substituted with phenyl. In certain embodiments, R 1  is substituted with C 3 -C 6 cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R 1  is substituted with C 1 -C 6 alkyl. Examples of C 1 -C 6 alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R 1  is substituted with haloC 1 -C 6 alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R 1  is substituted with C 1 -C 6 alkylOH. Examples of suitable alcohols, include, but are not limited to, hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl and iso-hydroxybutyl. In certain embodiments, R 1  is substituted with CON(R 7 )(R 8 ). In certain embodiments, R 1  is substituted with N(R 7 )(R 8 ). In certain embodiments, R 1  is substituted with C 1 -C 6 alkylN(R 7 )(R 8 ), wherein R 7  and R 8  will be described in detail below. 
     In certain embodiments, R 1  is substituted with 1 to 4 substituents selected independently from the group consisting of halogen, OH, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylOH, and phenyl. 
     In certain embodiments, R 1  is substituted with 1 to 4 substituents selected independently from the group consisting of phenyl, bromine, fluorine, chlorine, methyl, OH, cyclopropyl, spirocyclobutyl, cyclobutyl, CN, hydroxymethyl, oxo, methoxymethyl, COOCH 2 CH 3  and trifluoromethyl. 
     In certain embodiments, R 1  is 
     
       
         
         
             
             
         
       
     
     wherein each occurrence of R 1  is independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, oxo, COOC 1 -C 6 alkyl, phenyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) and C 1 -C 6 alkylN(R 7 )(R 8 ); and wherein q is 0, 1, 2, 3 or 4. In certain embodiments, each occurrence of R 1  is independently is selected independently from the group consisting of halogen, OH, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylOH, and phenyl. In certain embodiments, each occurrence of R 1  is independently selected independently from the group consisting of phenyl, bromine, fluorine, chlorine, methyl, OH, cyclopropyl, cyclobutyl, spirocyclobutyl, halogen, CN, hydroxymethyl, oxo, methoxymethyl, COOCH 2 CH 3  and trifluoromethyl. 
     In certain embodiments described herein, R 2  is hydrogen, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl or C 1 -C 6 alkylOH. 
     In certain embodiments, R 2  is hydrogen. In certain embodiments, R 2  is C 1 -C 6 alkylCOOH. In certain embodiments, R 2  is COOH. In certain embodiments, R 2  is C 3 -C 6 cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R 2  is C 1 -C 6 alkyl. Examples of C 1 -C 6 alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. 
     In certain embodiments, R 2  is haloC 1 -C 6 alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R 2  is C 1 -C 6 alkylOH. Examples of suitable alcohols substituents, include, but are not limited to, hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl and iso-hydroxybutyl. 
     With regard to the compounds described herein, R 3  is halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) or C 1 -C 6 alkylN(R 7 )(R 8 ). In certain embodiments, R 3  is halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, R 3  is CN. In certain embodiments, R 3  is OH. 
     In certain embodiments, R 3  is alkoxy. Suitable alkoxys include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. In certain embodiments, R 3  is C 1 -C 6 alkylOC 1 -C 6 alkyl. In certain embodiments, R 3  is COOH. In certain embodiments, R 3  is C 1 -C 6 alkylCOOH. In certain embodiments, R 3  is C 3 -C 6 cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R 3  is C 1 -C 6 alkyl. Examples of C 1 -C 6 alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R 3  is haloC 1 -C 6 alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R 3  is trifluoromethyl. In certain embodiments, R 3  is C 1 -C 6 alkylOH. Examples of suitable alcohols include, but are not limited to, hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl and iso-hydroxybutyl. In certain embodiments, R 3  is CON(R 7 )(R 1 ). In certain embodiments, R 3  is N(R 7 )(R 1 ). In certain embodiments, R 3  is C 1 -C 6 alkylN(R 7 )(R 8 ). R 7  and R 8  will be discussed in detail below. 
     In certain embodiments, R 3  is trifluoromethyl, fluorine or CN. 
     In certain embodiments, R 3  is fluorine or CN. 
     With regard to the compounds described here, any of the substitutable hydrogens on the ring containing U, X, Y and Z can be replaced by R 3 . In certain embodiments, n is 0, meaning the ring containing U, X, Y and Z is unsubstituted. In certain embodiments, n is 1, meaning a single substitutable hydrogen on the ring containing U, X, Y and Z is replaced by R 3 . In certain embodiments, n is 2, meaning two substitutable hydrogens on the ring containing U, X, Y and Z are replaced by R 3 . In certain embodiments, n is 3, meaning three substitutable hydrogens on the ring containing U, X, Y and Z are replaced by R 3 . 
     With regard to the compounds described herein, R 4  is hydrogen, halogen, CN, C 1 -C 6 alkylCN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylOhaloC 1 -C 6 alkyl, C 1 -C 6 alkylOC 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, C 1 -C 6 alkylSO 2 C 1 -C 6 alkyl, C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, C 1 -C 6 alkenyl, C 1 -C 6 alkynyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ), C 1 -C 6 alkyl(OCH 2 CH 2 ) m N 3 , or C 1 -C 6 alkylN(R 7 )(R 8 ), wherein the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl, C 1 -C 6 alkylOH, C 1 -C 6 alkylOC 1 -C 6 alkyl or C 3 -C 6 cycloalkyl is unsubstituted or substituted with 1 to 3 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, oxo, COOC 1 -C 6 alkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, SO 2 C 1 -C 6 alkyl, C 1 -C 6 alkySO 2 C 1 -C 6 alkyl, OSO 2 F, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) and C 1 -C 6 alkylN(R 7 )(R 8 ). 
     In certain embodiments, R 4  is hydrogen, halogen, CN, C 1 -C 6 alkylCN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylOhaloC 1 -C 6 alkyl, C 1 -C 6 alkylOC 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, C 1 -C 6 alkylSO 2 C 1 -C 6 alkyl, C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, C 1 -C 6 alkenyl, C 1 -C 6 alkynyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ), C 1 -C 6 alkyl(OCH 2 CH 2 ) m N 3 , or C 1 -C 6 alkylN(R 7 )(R 8 ), wherein the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is unsubstituted or substituted with 1 to 3 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, oxo, COOC 1 -C 6 alkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, SO 2 C 1 -C 6 alkyl, C 1 -C 6 alkySO 2 C 1 -C 6 alkyl, OSO 2 F, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) and C 1 -C 6 alkylN(R 7 )(R 8 ). 
     In certain embodiments, R 4  is hydrogen. In certain embodiments, R 4  is halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, R 4  is CN. In certain embodiments, R 4  is C 1 -C 6 alkylCN. Suitable C 1 -C 6 alkylCN include but are not limited to, CH 2 CN or CH 2 CH 2 CN. In certain embodiments, R 4  is OH. 
     In certain embodiments, R 4  is alkoxy. Suitable alkoxys include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. In certain embodiments, R 4  is C 1 -C 6 alkylOC 1 -C 6 alkyl. In certain embodiments, R 4  is C 1 -C 6 alkylOhaloC 1 -C 6 alkyl. In certain embodiments, R 4  is C 1 -C 6 alkylOC 1 -C 6 alkylOC 1 -C 6 alkyl. In certain embodiments, R 4  is COOH. In certain embodiments, R 4  is C 1 -C 6 alkylCOOH. In certain embodiments, R 4  is C 1 -C 6 alkylSO 2 C 1 -C 6 alkyl. 
     In certain embodiments, R 4  is phenyl. In certain embodiments, R 4  is C 1 -C 6 alkylphenyl. In certain embodiments, R 4  is a C 1 -C 6 alkylheterocycloalkyl. In certain embodiments, R 4  is a heterocycloalkyl. Non-limiting examples of heterocycloalkyl groups include piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof. 
     In certain embodiments, R 4  is C 3 -C 6 cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and (bicyclo[1.1.1]pentane). In certain embodiments, R 4  is C 1 -C 6 alkyl. Examples of C 1 -C 6 alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R 4  is haloC 1 -C 6 alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R 4  is C 1 -C 6 alkenyl. Suitable alkenyls include, but are not limited to, ethenyl, propenyl, butenyl, and hexenyl. In certain embodiments, R 4  is C 1 -C 6 alkynyl. Suitable alkynyls include, but are not limited to, ethynyl, propynyl, butynyl, and hexynyl. 
     In certain embodiments, R 4  is C 1 -C 6 alkylOH. Examples of suitable alcohols include, but are not limited to, hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl and iso-hydroxybutyl. In certain embodiments, R 4  is C 1 -C 6 alkyl(OCH 2 CH 2 ) m N 3 . In certain embodiments, R 4  is CON(R 7 )(R 8 ). In certain embodiments, R 4  is N(R 7 )(R 8 ). In certain embodiments, R 4  is C 1 -C 6 alkylN(R 7 )(R 8 ). R 7  and R 8  will be discussed in detail below. 
     In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is unsubstituted. 
     In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with 1 to 3 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, oxo, COOC 1 -C 6 alkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, SO 2 C 1 -C 6 alkyl, C 1 -C 6 alkySO 2 C 1 -C 6 alkyl, OSO 2 F, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) and C 1 -C 6 alkylN(R 7 )(R 8 ). 
     In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with CN. In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with OH. 
     In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with alkoxy. Suitable alkoxys include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with C 1 -C 6 alkylOC 1 -C 6 alkyl. 
     In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with COOH. In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with C 1 -C 6 alkylCOOH. In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with oxo. In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with COOC 1 -C 6 alkyl. 
     In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with C 1 -C 6 alkyl. Examples of C 1 -C 6 alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with haloC 1 -C 6 alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with C 1 -C 6 alkylOH. Examples of suitable alcohols include, but are not limited to, hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl and iso-hydroxybutyl. 
     In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with SO 2 C 1 -C 6 alkyl. In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with C 1 -C 6 alkylSO 2 C 1 -C 6 alkyl. In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with OSO 2 F. 
     In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with CON(R 7 )(R 8 ). In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with N(R 7 )(R 8 ). In certain embodiments, wherein R 4  is C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl, the C 1 -C 6 alkylphenyl, phenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, heteroaryl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is substituted with C 1 -C 6 alkylN(R 7 )(R 8 ). R 7  and R 8  will be discussed in detail below. 
     In certain embodiments, R 4  is C 1 -C 6 alkylOH or C 1 -C 6 alkylOC 1 -C 6 alkyl, wherein the C 1 -C 6 alkylOH or C 1 -C 6 alkylOC 1 -C 6 alkyl is substituted with haloC 1 -C 6 alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R 4  is C 1 -C 6 alkylOH or C 1 -C 6 alkylOC 1 -C 6 alkyl, wherein the C 1 -C 6 alkylOH or C 1 -C 6 alkylOC 1 -C 6 alkyl is substituted with trifluoromethyl. In certain embodiments, R 4  is 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 4  is hydrogen, phenyl, halogen, C 1 -C 6 alkyl, heteroaryl, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylOhaloC 1 -C 6 alkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkylphenyl, C 1 -C 6 alkenyl, C 1 -C 6 alkynyl, C 1 -C 6 alkylheterocycloalkyl, C 1 -C 6 alkylOH, C 1 -C 6 alkylCN, C 1 -C 6 alkylheteroaryl, C 1 -C 6 alkylOC 1 -C 6 alkylOC 1 -C 6 alkyl, heterocycloalkyl, C 1 -C 6 alkylSO 2 C 1 -C 6 alkyl, or C 1 -C 6 alkyl(OCH 2 CH 2 ) m N 3 , wherein the phenyl, C 1 -C 6 alkylphenyl, heterocycloalkyl, C 1 -C 6 alkylheterocycloalkyl, C 1 -C 6 alkylheteroaryl or C 3 -C 6 cycloalkyl is unsubstituted or substituted with 1 to 3 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, SO 2 C 1 -C 6 alkyl, C 1 -C 6 alkySO 2 C 1 -C 6 alkyl, and —OSO 2 F. 
     In certain embodiments R 4  is hydrogen, fluorine, methyl, ethyl, propyl, methoxymethyl, methoxypropyl, phenyl, pyridine, n-butyl, isobutyl, methoxyethyl, bicyclopentanyl, butenyl, butynyl, hydroxypropyl, cyanopropyl, hexynyl, methylbutadienyl, thiazole, pentynyl, CH 2 CH 2 OCH 3 , CH 2 CH 2 OCH 2 CH 3 , CH 2 OCH 2 CH 2 OCH 3 , CH 2 CH 2 CN, SO 2 CH 3 , CH 2 SO 2 CH 3 , CH 2 CH 2 SO 2 CH 3 , pyrimidine, 
     
       
         
         
             
             
         
       
     
     In certain embodiments R 4  is hydrogen, fluorine, methyl, ethyl, propyl, methoxymethyl, methoxypropyl, phenyl, pyridine, n-butyl, isobutyl, methoxyethyl, bicyclopentanyl, butenyl, butynyl, hydroxypropyl, cyanopropyl, hexynyl, methylbutadienyl, thiazole, pentynyl, CH 2 CH 2 OCH 3 , CH 2 CH 2 OCH 2 CH 3 , CH 2 OCH 2 CH 2 OCH 3 , CH 2 CH 2 CN, SO 2 CH 3 , CH 2 SO 2 CH 3 , CH 2 CH 2 SO 2 CH 3 , pyrimidine, 
     
       
         
         
             
             
         
       
     
     In certain embodiments R 4  is pyridine substituted with fluorine. In certain embodiments R 4  is isopropyl substituted with pyridine. In certain embodiments R 4  is phenyl substituted with SO 2 CH 3 . In certain embodiments R 4  is phenyl substituted with OSO 2 F. 
     With regard to the compounds described herein, R 5  is hydrogen, halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, phenyl, C 1 -C 6 alkylC 3 -C 6 cycloalkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) or C 1 -C 6 alkylN(R 7 )(R 8 ) or when R 5  is C 1 -C 6 alkyl and R 1  is a heterocycloalkyl or C 3 -C 12 cycloalkyl, R 5  optionally bonds to R 1  to form a macrocycle. In certain embodiments, R 5  is hydrogen. In certain embodiments, R 5  is halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, R 5  is CN. In certain embodiments, R 5  is OH. 
     In certain embodiments, R 5  is alkoxy. Suitable alkoxys include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. In certain embodiments, R 5  is C 1 -C 6 alkylOC 1 -C 6 alkyl. In certain embodiments, R 5  is COOH. In certain embodiments, R 5  is C 1 -C 6 alkylCOOH. In certain embodiments, R 5  is phenyl. In certain embodiments, R 5  is C 1 -C 6 alkylC 3 -C 6 cycloalkyl. In certain embodiments, R 5  is C 3 -C 6 cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R 5  is C 1 -C 6 alkyl. Examples of C 1 -C 6 alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl, n-heptyl and n-octyl. 
     In certain embodiments, R 5  is haloC 1 -C 6 alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R 5  is C 1 -C 6 alkylOH. Examples of suitable alcohols include, but are not limited to, hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl and iso-hydroxybutyl. In certain embodiments, R 5  is CON(R 7 )(R 8 ). In certain embodiments, R 5  is N(R 7 )(R 8 ). In certain embodiments, R 5  is C 1 -C 6 alkylN(R 7 )(R 8 ). R 7  and R 8  will be discussed in detail below. 
     In certain embodiments, R 5  is taken with R 6  and forms a C 3 -C 6 cycloalkyl or C 3 -C 6 heterocycloalkyl. In certain embodiments, R 5  is taken with R 6  and forms a C 3 -C 6 cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R 5  is taken with R 6  and forms a C 3 -C 6 heterocycloalkyl. Suitable examples of heterocycloalkyls include, but are not limited to, piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof. 
     In certain embodiments, when R 5  is C 1 -C 6 alkyl and R 1  is a heterocycloalkyl or C 3 -C 12 cycloalkyl, R 5  optionally bonds to R 1  to form a macrocycle. In certain embodiments, when R 5  is C 6 -C 7 alkyl, and R 1  is a heterocycloalkyl or C 3 -C 12 cycloalkyl, R 5  optionally bonds to R 1  to form a macrocycle. In certain embodiments, when R 5  is C 7 alkyl, and R 1  is a heterocycloalkyl or C 3 -C 12 cycloalkyl, R 5  optionally bonds to R 1  to form a macrocycle, as shown in Formula II 
     
       
         
         
             
             
         
       
     
     In certain embodiments, R 5  is trifluoromethyl, methoxymethyl, methyl, ethyl, C 6 -C 7 alkyl, isopropyl, 
     
       
         
         
             
             
         
       
     
     or t-butyl. 
     With regard to the compounds described herein, R 6  is hydrogen, halogen, CN, OH, alkoxy, C 1 -C 6 alkylOC 1 -C 6 alkyl, C 1 -C 6 alkylCOOH, COOH, phenyl, C 1 -C 6 alkylC 3 -C 6 cycloalkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl, C 1 -C 6 alkylOH, CON(R 7 )(R 8 ), N(R 7 )(R 8 ) or C 1 -C 6 alkylN(R 7 )(R 8 ) or when taken with R 5  forms a C 3 -C 6 cycloalkyl or C 3 -C 6 heterocycloalkyl. In certain embodiments, R 6  is hydrogen. In certain embodiments, R 6  is halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, R 6  is CN. In certain embodiments, R 6  is OH. 
     In certain embodiments, R 6  is alkoxy. Suitable alkoxys include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. In certain embodiments, R 6  is C 1 -C 6 alkylalkoxy. In certain embodiments, R 6  is COOH. In certain embodiments, R 6  is C 1 -C 6 alkylCOOH. In certain embodiments, R 6  is phenyl. In certain embodiments, R 6  is C 1 -C 6 alkylC 3 -C 6 cycloalkyl. In certain embodiments, R 6  is C 3 -C 6 cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R 6  is C 1 -C 6 alkyl. Examples of C 1 -C 6 alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R 6  is haloC 1 -C 6 alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R 6  is C 1 -C 6 alkylOH. Examples of suitable alcohols include, but are not limited to, hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl and iso-hydroxybutyl. In certain embodiments, R 6  is CON(R 7 )(R 8 ). In certain embodiments, R 6  is N(R 7 )(R 8 ). In certain embodiments, R 6  is C 1 -C 6 alkylN(R 7 )(R 8 ). R 7  and R 8  will be discussed in detail below. 
     In certain embodiments, R 6  is taken with R 5  and forms a C 3 -C 6 cycloalkyl or C 3 -C 6 heterocycloalkyl. In certain embodiments, R 6  is taken with R 5  and forms a C 3 -C 6 cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R 6  is taken with R 5  and forms a C 3 -C 6 heterocycloalkyl. Suitable examples of heterocycloalkyls include, but are not limited to, piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof. 
     In certain embodiments, R 6  is methyl, phenyl, ethyl, isopropyl or t-butyl. 
     With regard to the compounds described herein, R 7  is hydrogen, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl or C 1 -C 6 alkylOH. 
     In certain embodiments, R 7  is hydrogen. In certain embodiments, R 7  is C 1 -C 6 alkylCOOH. In certain embodiments, R 7  is COOH. In certain embodiments, R 7  is C 3 -C 6 cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R 7  is C 1 -C 6 alkyl. Examples of C 1 -C 6 alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R 7  is haloC 1 -C 6 alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R 7  is C 1 -C 6 alkylOH. Examples of suitable alcohols include, but are not limited to, hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl and iso-hydroxybutyl. 
     With regard to the compounds described herein, R 8  is hydrogen, C 1 -C 6 alkylCOOH, COOH, C 3 -C 6 cycloalkyl, C 1 -C 6 alkyl, haloC 1 -C 6 alkyl or C 1 -C 6 alkylOH. 
     In certain embodiments, R 8  is hydrogen. In certain embodiments, R 8  is C 1 -C 6 alkylCOOH. In certain embodiments, R 8  is COOH. In certain embodiments, R 8  is C 3 -C 6 cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R 8  is C 1 -C 6 alkyl. Examples of C 1 -C 6 alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R 8  is haloC 1 -C 6 alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R 8  is C 1 -C 6 alkylOH. Examples of suitable alcohols include, but are not limited to, hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl and iso-hydroxybutyl. 
     With regard to the compounds described herein, R 9  is hydrogen, halogen, and C 1 -C 6 alkyl. In certain embodiments, R 9  is hydrogen. In certain embodiments, R 9  is halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, R 9  is C 1 -C 6 alkyl. Examples of C 1 -C 6 alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R 9  is hydrogen, fluorine or methyl. 
     With regard to the compounds described herein, m is 1, 2 or 3. In certain embodiments, m is 1. In certain embodiments, m is 2. In certain embodiments, m is 3. 
     In each of the various embodiments of the invention, in the compounds used in the methods herein, for each variable it shall be understood that each variable is to be selected independently of the others unless otherwise indicated. 
     In each of the various embodiments of the invention, the compounds described herein, including those in Formula (I) and the various embodiments thereof, may exist in different forms of the compounds such as, for example, any solvates, hydrates, stereoisomers, and tautomers of said compounds and of any pharmaceutically acceptable salts thereof. 
     In certain embodiments, compounds described herein include: 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     An asterisk (*) may be used in a chemical structure drawing that indicates the location of a chiral center. 
     In other embodiments described herein are the following compounds 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     or pharmaceutically acceptable salts thereof. 
     Definitions and Abbreviations 
     The terms used herein have their ordinary meaning and the meaning of such terms is independent at each occurrence thereof. That notwithstanding and except where stated otherwise, the following definitions apply throughout the specification and claims. Chemical names, common names and chemical structures may be used interchangeably to describe that same structure. These definitions apply regardless of whether a term is used by itself or in combination with other terms, unless otherwise indicated. Hence the definition of “alkyl” applies to “alkyl” as well as the “alkyl” portion of “hydroxyalkyl”, “haloalkyl”, arylalkyl-, alkylaryl-, “alkoxy” etc. 
     It shall be understood that, in the various embodiments of the invention described herein, any variable not explicitly defined in the context of the embodiment is as defined in Formula (I). 
     In the various embodiments described herein, each variable is selected independently of the others unless otherwise indicated. 
     “Drug resistant” means, in connection with a  Plasmodium  parasite strain, a  Plasmodium  species which is no longer susceptible to at least one previously effective drug; which has developed the ability to withstand attack by at least one previously effective drug. A drug resistant strain may relay that ability to withstand to its progeny. Said resistance may be due to random genetic mutations in the bacterial cell that alter its sensitivity to a single drug or to different drugs. 
     “Patient” includes both human and non-human animals. Non-human animals include those research animals and companion animals such as mice, rats, primates, monkeys, chimpanzees, great apes, dogs, and house cats. 
     “Pharmaceutical composition” (or “pharmaceutically acceptable composition”) means a composition suitable for administration to a patient. Such compositions may contain the neat compound (or compounds) of the invention or mixtures thereof, or salts, solvates, prodrugs, isomers, or tautomers thereof, and one or more pharmaceutically acceptable carriers or diluents. The term “pharmaceutical composition” is also intended to encompass both the bulk composition and individual dosage units comprised of one or more (e.g., two) pharmaceutically active agents such as, for example, a compound of the present invention and an additional agent selected from the lists of the additional agents described herein, along with any pharmaceutically inactive excipients. The bulk composition and each individual dosage unit can contain fixed amounts of the afore-said “more than one pharmaceutically active agents”. The bulk composition is material that has not yet been formed into individual dosage units. An illustrative dosage unit is an oral dosage unit such as tablets, pills and the like. Similarly, the herein-described method of treating a patient by administering a pharmaceutical composition of the present invention is also intended to encompass the administration of the afore-said bulk composition and individual dosage units. 
     “Halogen” and “halo” mean fluorine, chlorine, bromine, or iodine. Preferred are fluorine, chlorine and bromine. 
     “Alkyl” means an aliphatic hydrocarbon group which may be straight or branched and comprising about 1 to about 20 carbon atoms in the chain. Preferred alkyl groups contain about 1 to about 12 carbon atoms in the chain. More preferred alkyl groups contain about 1 to about 6 carbon atoms in the chain. Branched means that one or more lower alkyl groups such as methyl, ethyl or propyl, are attached to a linear alkyl chain. “Lower alkyl” means a group having about 1 to about 6 carbon atoms in the chain which may be straight or branched. Non-limiting examples of suitable alkyl groups include methyl, ethyl, n-propyl, isopropyl and t-butyl. 
     “Haloalkyl” means an alkyl as defined above wherein one or more hydrogen atoms on the alkyl is replaced by a halo group defined above. 
     “Aryl” means an aromatic monocyclic or multicyclic ring system comprising about 6 to about 14 carbon atoms, preferably about 6 to about 10 carbon atoms. The aryl group can be optionally substituted with one or more “ring system substituents” which may be the same or different, and are as defined herein. Non-limiting examples of suitable aryl groups include phenyl and naphthyl. “Monocyclic aryl” means phenyl. 
     “Cycloalkyl” means a non-aromatic mono- or multicyclic ring or bridged system comprising about 3 to about 12 carbon atoms, preferably about 3 to about 10 carbon atoms. Preferred cycloalkyl rings contain about 5 to about 10 ring atoms. The cycloalkyl can be optionally substituted with one or more substituents, which may be the same or different, as described herein. Monocyclic cycloalkyl refers to monocyclic versions of the cycloalkyl moieties described herein. Non-limiting examples of suitable monocyclic cycloalkyls include cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl and the like. Multicyclic cycloalkyls refers to multicyclic, including bicyclic, rings that include a non-aromatic ring. Non-limiting examples of suitable multicyclic cycloalkyls include 1-decalinyl, norbornyl, adamantyl and the like. In certain embodiments, a non-aromatic ring is fused to an aromatic ring. Further non-limiting examples of cycloalkyl include the following: bicyclo[1.1.1]pentanyl, 
     
       
         
         
             
             
         
       
     
     “Heterocycloalkyl” (or “heterocyclyl”) means a non-aromatic, saturated or partially saturated monocyclic or multicyclic ring system comprising about 3 to about 10 ring atoms, preferably about 5 to about 10 ring atoms, in which one or more of the atoms in the ring system is an element other than carbon, for example nitrogen, oxygen or sulfur, alone or in combination. There are no adjacent oxygen and/or sulfur atoms present in the ring system. Preferred heterocyclyls contain about 5 to about 6 ring atoms. The prefix aza, oxa or thia before the heterocyclyl root name means that at least a nitrogen, oxygen or sulfur atom respectively is present as a ring atom. Any —NH in a heterocyclyl ring may exist protected such as, for example, as an —N(Boc), —N(CBz), —N(Tos) group and the like; such protections are also considered part of this invention. The heterocyclyl can be optionally substituted by one or more substituents, which may be the same or different, as described herein. The nitrogen or sulfur atom of the heterocyclyl can be optionally oxidized to the corresponding N-oxide, S-oxide or S,S-dioxide. Thus, the term “oxide,” when it appears in a definition of a variable in a general structure described herein, refers to the corresponding N-oxide, S-oxide, or S,S-dioxide. “Heterocyclyl” also includes rings wherein ═O replaces two available hydrogens on the same carbon atom (i.e., heterocyclyl includes rings having a carbonyl group in the ring). Such ═O groups may be referred to herein as “oxo.” An example of such a moiety is pyrrolidinone (or pyrrolidone): 
     
       
         
         
             
             
         
       
     
     As used herein, the term “monocyclic heterocycloalkyl” refers monocyclic versions of the heterocycloalkyl moieties described herein and include a 4- to 7-membered monocyclic heterocycloalkyl groups comprising from 1 to 4 ring heteroatoms, said ring heteroatoms being independently selected from the group consisting of N, N-oxide, O, S, S-oxide, S(O), and S(O) 2 . The point of attachment to the parent moiety is to any available ring carbon or ring heteroatom. Non-limiting examples of monocyclic heterocycloalkyl groups include piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof. A non-limiting example of a monocyclic heterocycloalkyl group include the moiety: 
     
       
         
         
             
             
         
       
     
     Non-limiting examples of multicyclic heterocycloalkyl groups include, bicyclic heterocycloalkyl groups. Specific examples include, but are not limited to, 
     
       
         
         
             
             
         
       
     
     “Alkoxy” means an alkyl-O— group in which the alkyl group is as previously described. Non-limiting examples of suitable alkoxy groups include methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. The bond to the parent moiety is through the ether oxygen. 
     The term “substituted” means that one or more hydrogens on the designated atom is replaced with a selection from the indicated group, provided that the designated atom&#39;s normal valency under the existing circumstances is not exceeded, and that the substitution results in a stable compound. Combinations of substituents and/or variables are permissible only if such combinations result in stable compounds. By “stable compound” or “stable structure” is meant a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and formulation into an efficacious therapeutic agent. 
     The term “optionally substituted” means optional substitution with the specified groups, radicals or moieties. 
     When a variable appears more than once in a group, e.g., R 8  in —N(R 8 ) 2 , or a variable appears more than once in a structure presented herein, the variables can be the same or different. 
     A solid line  , as a bond generally indicates a mixture of, or either of, the possible isomers, e.g., containing (R)- and (S)-stereochemistry. For example: 
     
       
         
         
             
             
         
       
     
     means containing either one of or both 
     
       
         
         
             
             
         
       
     
     The wavy line  , as used herein shown crossing a line representing a chemical bond, indicates a point of attachment to the rest of the compound. Lines drawn into the ring systems, such as, for example 
     
       
         
         
             
             
         
       
     
     indicates that the indicated line (bond) may be attached to any of the substitutable ring atoms. 
     “Oxo” is defined as an oxygen atom that is double bonded to a ring carbon in a cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, or another ring described herein, e.g., 
     
       
         
         
             
             
         
       
     
     In this specification, where there are multiple oxygen and/or sulfur atoms in a ring system, there cannot be any adjacent oxygen and/or sulfur present in said ring system. 
     As well known in the art, a bond drawn from a particular atom wherein no moiety is depicted at the terminal end of the bond indicates a methyl group bound through that bond to the atom, unless stated otherwise. For example: 
     
       
         
         
             
             
         
       
     
     represents 
     
       
         
         
             
             
         
       
     
     In another embodiment, the compounds useful in the methods of the invention, and/or compositions comprising them useful in said methods, are present in isolated and/or purified form. The term “purified”, “in purified form” or “in isolated and purified form” for a compound refers to the physical state of said compound after being isolated from a synthetic process (e.g., from a reaction mixture), or natural source or combination thereof. Thus, the term “purified”, “in purified form” or “in isolated and purified form” for a compound refers to the physical state of said compound (or a tautomer or stereoisomer thereof, or pharmaceutically acceptable salt or solvate of said compound, said stereoisomer, or said tautomer) after being obtained from a purification process or processes described herein or well known to the skilled artisan (e.g., chromatography, recrystallization and the like), in sufficient purity to be suitable for in vivo or medicinal use and/or characterizable by standard analytical techniques described herein or well known to the skilled artisan. 
     It shall be understood that any carbon as well as heteroatom with unsatisfied valences in the text, schemes, examples and tables herein is assumed to have the sufficient number of hydrogen atom(s) to satisfy the valences. 
     When a functional group in a compound is termed “protected”, this means that the group is in modified form to preclude undesired side reactions at the protected site when the compound is subjected to a reaction. Suitable protecting groups will be recognized by those with ordinary skill in the art as well as by reference to standard textbooks such as, for example, T. W. Greene et al,  Protective Groups in Organic Synthesis  (1991), Wiley, New York. 
     Another embodiment provides prodrugs and/or solvates of the compounds of the invention. A discussion of prodrugs is provided in T. Higuchi and V. Stella,  Pro - drugs as Novel Delivery Systems  (1987) 14 of the A.C.S. Symposium Series, and in  Bioreversible Carriers in Drug Design , (1987) Edward B. Roche, ed., American Pharmaceutical Association and Pergamon Press. The term “prodrug” means a compound (e.g., a drug precursor) that is transformed in vivo to yield a compound of the invention or a pharmaceutically acceptable salt, hydrate or solvate of the compound. The transformation may occur by various mechanisms (e.g., by metabolic or chemical processes), such as, for example, through hydrolysis in blood. A discussion of the use of prodrugs is provided by T. Higuchi and W. Stella, “Pro-drugs as Novel Delivery Systems,” Vol. 14 of the A.C.S. Symposium Series, and in Bioreversible Carriers in Drug Design, ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, 1987. 
     For example, if a compound useful in the methods of the invention or a pharmaceutically acceptable salt thereof, contains a carboxylic acid functional group, a prodrug can comprise an ester formed by the replacement of the hydrogen atom of the acid group with a group such as, for example, (C 1 -C 8 )alkyl, (C 2 -C 12 )alkanoyloxymethyl, 1-(alkanoyloxy)ethyl having from 4 to 9 carbon atoms, 1-methyl-1-(alkanoyloxy)-ethyl having from 5 to 10 carbon atoms, alkoxycarbonyloxymethyl having from 3 to 6 carbon atoms, 1-(alkoxycarbonyloxy)ethyl having from 4 to 7 carbon atoms, 1-methyl-1-(alkoxycarbonyloxy)ethyl having from 5 to 8 carbon atoms, N-(alkoxycarbonyl)aminomethyl having from 3 to 9 carbon atoms, 1-(N-(alkoxycarbonyl)amino)ethyl having from 4 to 10 carbon atoms, 3-phthalidyl, 4-crotonolactonyl, gamma-butyrolacton-4-yl, di-N,N—(C 1 -C 2 )alkylamino(C 2 -C 3 )alkyl (such as β-dimethylaminoethyl), carbamoyl-(C 1 -C 2 )alkyl, N,N-di (C 1 -C 2 )alkylcarbamoyl-(C 1 -C 2 )alkyl and piperidino-, pyrrolidino- or morpholino(C 2 -C 3 )alkyl, and the like. 
     Similarly, if a compound used in the methods of the invention contains an alcohol functional group, a prodrug can be formed by the replacement of the hydrogen atom of the alcohol group with a group such as, for example, (C 1 -C 6 )alkanoyloxymethyl, 1-((C 1 -C 6 )alkanoyloxy)ethyl, 1-methyl-1-((C 1 -C 6 )alkanoyloxy)ethyl, (C 1 -C 6 )alkoxycarbonyloxymethyl, N—(C 1 -C 6 )alkoxycarbonylaminomethyl, succinoyl, (C 1 -C 6 )alkanoyl, α-amino(C 1 -C 4 )alkanyl, arylacyl and α-aminoacyl, or α-aminoacyl-α-aminoacyl, where each α-aminoacyl group is independently selected from the naturally occurring L-amino acids, P(O)(OH) 2 , —P(O)(O(C 1 -C 6 )alkyl) 2  or glycosyl (the radical resulting from the removal of a hydroxyl group of the hemiacetal form of a carbohydrate), and the like. 
     If a compound used in the methods of the invention incorporates an amine functional group, a prodrug can be formed by the replacement of a hydrogen atom in the amine group with a group such as, for example, R-carbonyl, RO-carbonyl, NRR′-carbonyl where R and R′ are each independently (C 1 -C 10 )alkyl, (C 3 -C 7 ) cycloalkyl, benzyl, or R-carbonyl is a natural α-aminoacyl or natural α-aminoacyl, —C(OH)C(O)OY 1  wherein Y 1  is H, (C 1 -C 6 )alkyl or benzyl, —C(OY 2 )Y 3  wherein Y 2  is (C 1 -C 4 ) alkyl and Y 3  is (C 1 -C 6 )alkyl, carboxy (C 1 -C 6 )alkyl, amino(C 1 -C 4 )alkyl or mono-N- or di-N,N—(C 1 -C 6 )alkylaminoalkyl, —C(Y 4 )Y 5  wherein Y 4  is H or methyl and Y 5  is mono-N- or di-N,N—(C 1 -C 6 )alkylamino morpholino, piperidin-1-yl or pyrrolidin-1-yl, and the like. 
     One or more compounds used in the methods of the invention may exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like, and it is intended that the invention embrace both solvated and unsolvated forms. “Solvate” means a physical association of a compound of the invention with one or more solvent molecules. This physical association involves varying degrees of ionic and covalent bonding, including hydrogen bonding. In certain instances, the solvate will be capable of isolation, for example when one or more solvent molecules are incorporated in the crystal lattice of the crystalline solid. “Solvate” encompasses both solution-phase and isolatable solvates. Non-limiting examples of suitable solvates include ethanolates, methanolates, and the like. “Hydrate” is a solvate wherein the solvent molecule is H 2 O. 
     One or more compounds used in the methods of the invention may optionally be converted to a solvate. Preparation of solvates is generally known. Thus, for example M. Caira et al,  J. Pharmaceutical Sci.,  1993, 3, 601-611, describe the preparation of the solvates of the antifungal fluconazole in ethyl acetate as well as from water. Similar preparations of solvates, hemisolvate, hydrates and the like are described by E. C. van Tonder et al,  AAPS PharmSciTech.,  5(1), article 12 (2004); and A. L. Bingham et al,  Chem. Commun.,  603-604 (2001). A typical, non-limiting, process involves dissolving the inventive compound in desired amounts of the desired solvent (organic or water or mixtures thereof) at a higher than ambient temperature, and cooling the solution at a rate sufficient to form crystals which are then isolated by standard methods. Analytical techniques such as, for example I. R. spectroscopy, show the presence of the solvent (or water) in the crystals as a solvate (or hydrate). 
     “Effective amount” or “therapeutically effective amount” is meant to describe an amount of compound or a composition used in the methods of the present invention effective in inhibiting the above-noted diseases or enzyme activity and thus producing the desired therapeutic, ameliorative, inhibitory or preventative effect. 
     Another embodiment provides pharmaceutically acceptable salts of the compounds to be used in the methods of the invention. Thus, reference to a compound used in the methods of the invention herein is understood to include reference to salts thereof, unless otherwise indicated. The term “salt(s)”, as employed herein, denotes acidic salts formed with inorganic and/or organic acids, as well as basic salts formed with inorganic and/or organic bases. In addition, when a compound of the invention contains both a basic moiety, such as, but not limited to a pyridine or imidazole, and an acidic moiety, such as, but not limited to a carboxylic acid, zwitterions (“inner salts”) may be formed and are included within the term “salt(s)” as used herein. Pharmaceutically acceptable (i.e., non-toxic, physiologically acceptable) salts are preferred, although other salts are also useful. Salts of the compounds used in the methods of the invention may be formed, for example, by reacting a compound of the invention with an amount of acid or base, such as an equivalent amount, in a medium such as one in which the salt precipitates or in an aqueous medium followed by lyophilization. 
     Exemplary acid addition salts include acetates, ascorbates, benzoates, benzenesulfonates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, fumarates, hydrochlorides, hydrobromides, hydroiodides, lactates, maleates, methanesulfonates, naphthalenesulfonates, nitrates, oxalates, phosphates, propionates, salicylates, succinates, sulfates, tartarates, thiocyanates, toluenesulfonates (also known as tosylates,) and the like. 
     Additionally, acids which are generally considered suitable for the formation of pharmaceutically useful salts from basic pharmaceutical compounds are discussed, for example, by P. Stahl et al, Camille G. (eds.)  Handbook of Pharmaceutical Salts. Properties, Selection and Use . (2002) Zurich: Wiley-VCH; S. Berge et al,  Journal of Pharmaceutical Sciences  (1977) 66(1) 1-19; P. Gould,  International J. of Pharmaceutics  (1986) 33 201-217; Anderson et al,  The Practice of Medicinal Chemistry  (1996), Academic Press, New York; and in  The Orange Book  (Food &amp; Drug Administration, Washington, D.C. on their website). These disclosures are incorporated herein by reference thereto. 
     Exemplary basic salts include ammonium salts, alkali metal salts such as sodium, lithium, and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, salts with organic bases (for example, organic amines) such as dicyclohexylamines, t-butyl amines, and salts with amino acids such as arginine, lysine and the like. Basic nitrogen-containing groups may be quarternized with agents such as lower alkyl halides (e.g. methyl, ethyl, and butyl chlorides, bromides and iodides), dialkyl sulfates (e.g. dimethyl, diethyl, and dibutyl sulfates), long chain halides (e.g. decyl, lauryl, and stearyl chlorides, bromides and iodides), aralkyl halides (e.g. benzyl and phenethyl bromides), and others. 
     All such acid salts and base salts are intended to be pharmaceutically acceptable salts within the scope of the invention and all acid and base salts are considered equivalent to the free forms of the corresponding compounds for purposes of the invention. 
     Another embodiment provides pharmaceutically acceptable esters of the compounds used in the methods of the invention. Such esters include the following groups: (1) carboxylic acid esters obtained by esterification of the hydroxy groups, in which the non-carbonyl moiety of the carboxylic acid portion of the ester grouping is selected from straight or branched chain alkyl (for example, acetyl, n-propyl, t-butyl, or n-butyl), alkoxyalkyl (for example, methoxymethyl), aralkyl (for example, benzyl), aryloxyalkyl (for example, phenoxymethyl), aryl (for example, phenyl optionally substituted with, for example, halogen, C 1-4 alkyl, or C 1-4 alkoxy or amino); (2) sulfonate esters, such as alkyl- or aralkylsulfonyl (for example, methanesulfonyl); (3) amino acid esters (for example, L-valyl or L-isoleucyl); (4) phosphonate esters and (5) mono-, di- or triphosphate esters. The phosphate esters may be further esterified by, for example, a C 1-20  alcohol or reactive derivative thereof, or by a 2,3-di (C 6-24 )acyl glycerol. 
     As mentioned herein, another embodiment provides tautomers of the compounds of the invention to be used in the methods herein, and salts, solvates, esters and prodrugs of said tautomers. It shall be understood that all tautomeric forms of such compounds are within the scope of the compounds used in the methods of the invention. For example, all keto-enol and imine-enamine forms of the compounds, when present, are included in the invention. 
     The compounds used in the methods of the invention may contain asymmetric or chiral centers, and, therefore, exist in different stereoisomeric forms. It is intended that all stereoisomeric forms of the compounds used in the methods of the invention as well as mixtures thereof, including racemic mixtures, form part of the present invention. In addition, the present invention embraces use of all geometric and positional isomers. For example, if a compound used in the methods of the invention incorporates a double bond or a fused ring, both the cis- and trans-forms, as well as mixtures, are embraced within the scope of the invention. 
     Another embodiment provides for diastereomeric mixtures and individual enantiomers of the compounds used in the methods of the invention. Diastereomeric mixtures can be separated into their individual diastereomers based on their physical chemical differences by methods well known to those skilled in the art, such as, for example, by chromatography and/or fractional crystallization. Enantiomers can be separated by converting the enantiomeric mixture into a diastereomeric mixture by reaction with an appropriate optically active compound (e.g., chiral auxiliary such as a chiral alcohol or Mosher&#39;s acid chloride), separating the diastereomers and converting (e.g., hydrolyzing) the individual diastereomers to the corresponding pure enantiomers. Also, some of the compounds used in the methods of the invention may be atropisomers (e.g., substituted biaryls) and are considered as part of this invention. Enantiomers can also be separated by use of chiral HPLC column. 
     All stereoisomers (for example, geometric isomers, optical isomers and the like) of the compounds used in the methods of the invention (including those of the salts, solvates, esters and prodrugs of the compounds as well as the salts, solvates and esters of the prodrugs), such as those which may exist due to asymmetric carbons on various substituents, including enantiomeric forms (which may exist even in the absence of asymmetric carbons), rotameric forms, atropisomers, and diastereomeric forms, are contemplated as embodiments within the scope of this invention, as are positional isomers (such as, for example, 4-pyridyl and 3-pyridyl). (For example, if a compound of the invention incorporates a double bond or a fused ring, both the cis- and trans-forms, as well as mixtures, are embraced within the scope of the invention. Also, for example, all keto-enol and imine-enamine forms of the compounds are included compounds and methods of using such compounds described herein). 
     Individual stereoisomers of the compounds of the invention may, for example, be substantially free of other isomers, or may be admixed, for example, as racemates or with all other, or other selected, stereoisomers. The chiral centers of the present invention can have the S or R configuration as defined by the IUPAC 1974 Recommendations. The use of the terms “salt”, “solvate”, “ester”, “prodrug” and the like, is intended to equally apply to the salt, solvate, ester and prodrug of enantiomers, stereoisomers, rotamers, tautomers, positional isomers, racemates or prodrugs of the inventive compounds. 
     Another embodiment provides isotopically-labelled compounds to be used in the methods the invention. Such compounds are identical to those recited herein, but for the fact that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, fluorine and chlorine, such as  2 H,  3 H,  13 C,  14 C,  15 N,  18 O,  17 O  31 P,  32 P,  35 S,  18 F, and  36 Cl, respectively. 
     Certain isotopically-labelled compounds of the invention (e.g., those labeled with  3 H and  14 C) are useful in compound and/or substrate tissue distribution assays. Tritiated (i.e.,  3 H) and carbon-14 (i.e.,  14 C) isotopes are particularly preferred for their ease of preparation and detectability. Further, substitution with heavier isotopes such as deuterium (i.e.,  2 H) may afford certain therapeutic advantages resulting from greater metabolic stability (e.g., increased in vivo half-life or reduced dosage requirements) and hence may be preferred in some circumstances. Isotopically labelled compounds of the invention can generally be prepared by following procedures analogous to those disclosed in the Schemes and/or in the Examples hereinbelow, by substituting an appropriate isotopically labelled reagent for a non-isotopically labelled reagent. 
     In the compounds used in the methods of the invention, the atoms may exhibit their natural isotopic abundances, or one or more of the atoms may be artificially enriched in a particular isotope having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number predominantly found in nature. The present invention is meant to include all suitable isotopic variations of the compounds of the invention. For example, different isotopic forms of hydrogen (H) include protium ( 1 H) and deuterium ( 2 H). The presence of deuterium in the compounds of the invention is indicated by “D”. Protium is the predominant hydrogen isotope found in nature. Enriching for deuterium may afford certain therapeutic advantages, such as increasing in vivo half-life or reducing dosage requirements, or may provide a compound useful as a standard for characterization of biological samples. Isotopically-enriched compounds of the invention can be prepared without undue experimentation by conventional techniques well known to those skilled in the art or by processes analogous to those described in the schemes and examples herein using appropriate isotopically-enriched reagents and/or intermediates. 
     Polymorphic forms of the compounds used in the methods of the invention, and of the salts, solvates, esters and prodrugs of the compounds of the invention, are intended to be included in the present invention. 
     Methods of Treatment 
     The present invention is directed to methods of treatment of  Plasmodium  infections comprising administering to a subject in need thereof a compound described herein, or a pharmaceutically acceptable salt thereof. More specifically, the methods of the invention comprise administration of a compound of Formula (I), or a pharmaceutically acceptable salt thereof. In certain embodiments, the compounds of Formula (I), or a pharmaceutically acceptable salt thereof, are administered in the form of a pharmaceutical composition, further comprising a pharmaceutically acceptable carrier or excipient. 
     The present invention provides a method for treating a  Plasmodium  infection, or for treating malaria, or for inhibiting plasmepsin X which comprises administering to a subject in need of such treatment a therapeutically effective amount of a compound, or a pharmaceutically acceptable salt thereof, said compound having the structural Formula (I) described in the Summary of the Invention. In some embodiments, the compounds of Formula (I), or pharmaceutically acceptable salts thereof, are administered with a pharmaceutically acceptable carrier, as a pharmaceutical composition. Also provided herein are various embodiments of these methods, as described, infra. 
     The invention also relates to the use of a compound of Formula (I) or a pharmaceutically acceptable salt thereof for inhibiting plasmepsin X activity, for treating a  Plasmodium  infection, or for treating malaria. The invention further relates to the use of a compound of Formula (I) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for inhibiting plasmepsin X activity, for treating a  Plasmodium  infection, or for treating malaria. The compounds of Formula (I) or pharmaceutically acceptable salts thereof described in any of the embodiments of the invention herein are useful for any of the uses above. 
     The present invention provides a method for treating a  Plasmodium  infection, or for treating malaria, or for inhibiting plasmepsin IX which comprises administering to a subject in need of such treatment a therapeutically effective amount of a compound, or a pharmaceutically acceptable salt thereof, said compound having the structural Formula (I) described in the Summary of the Invention. In some embodiments, the compounds of Formula (I), or pharmaceutically acceptable salts thereof, are administered with a pharmaceutically acceptable carrier, as a pharmaceutical composition. Also provided herein are various embodiments of these methods, as described, infra. 
     The invention also relates to the use of a compound of Formula (I) or a pharmaceutically acceptable salt thereof for inhibiting plasmepsin IX activity, for treating a  Plasmodium  infection, or for treating malaria. The invention further relates to the use of a compound of Formula (I) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for inhibiting plasmepsin IX activity, for treating a  Plasmodium  infection, or for treating malaria. The compounds of Formula (I) or pharmaceutically acceptable salts thereof described in any of the embodiments of the invention herein are useful for any of the uses above. 
     The present invention provides a method for treating a  Plasmodium  infection, or for treating malaria, or for inhibiting plasmepsin X and plasmepsin IX which comprises administering to a subject in need of such treatment a therapeutically effective amount of a compound, or a pharmaceutically acceptable salt thereof, said compound having the structural Formula (I) described in the Summary of the Invention. In some embodiments, the compounds of Formula (I), or pharmaceutically acceptable salts thereof, are administered with a pharmaceutically acceptable carrier, as a pharmaceutical composition. Also provided herein are various embodiments of these methods, as described, infra. 
     The invention also relates to the use of a compound of Formula (I) or a pharmaceutically acceptable salt thereof for inhibiting plasmepsin X and plasmepsin IX activity, for treating a  Plasmodium  infection, or for treating malaria. The invention further relates to the use of a compound of Formula (I) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for inhibiting plasmepsin X and plasmepsin IX activity, for treating a  Plasmodium  infection, or for treating malaria. The compounds of Formula (I) or pharmaceutically acceptable salts thereof described in any of the embodiments of the invention herein are useful for any of the uses above. 
     The methods of the present invention are useful for treating malaria in that they inhibit the onset, growth, or progression of the condition, ameliorate the symptoms of the condition, cause regression of the condition, cure the condition, or otherwise improve the general well-being of a subject afflicted with, or at risk of, contracting the condition. Thus, in accordance with the presently disclosed subject matter, the terms “treat”, “treating”, and grammatical variations thereof, as well as the phrase “method of treating”, are meant to encompass any desired therapeutic intervention, including but not limited to a method for treating an existing infection in a subject of infection, such as in a subject that has been exposed to a parasite as disclosed herein. 
     Embodiments of the invention also include one or more of the compounds of Formula (I) or a pharmaceutically acceptable salt thereof (i) for use in, (ii) for use as a medicament or composition for, or (iii) for use in the preparation of a medicament for: (a) therapy (e.g., of the human body); (b) medicine; (c) inhibition of parasite/ Plasmodium  growth, (d) treatment or prophylaxis of infection by  Plasmodium  species; (e) reduction of the progression, onset or severity of pathological symptoms associated with  Plasmodium  infection and/or reduction of the likelihood of severe  Plasmodium  infection or, (f) treatment, prophylaxis of, or delay in the onset, severity, or progression of  Plasmodium -associated disease(s), including, but not limited to: malaria. 
     Accordingly, another embodiment provides methods for the treatment of malaria or for the treatment of  Plasmodium  infection, comprising administration of combinations comprising an amount of at least one compound of Formula (I), or a pharmaceutically acceptable salt, solvate, ester or prodrug thereof, and an effective amount of one or more additional agents described below. In certain embodiments, described herein are methods for the treatment of malaria or for the treatment of  Plasmodium  infection, comprising administration of combinations comprising an amount of at least one compound of Formula (I), or a pharmaceutically acceptable salt, solvate, ester or prodrug thereof, and an effective amount of one or more additional anti-malarial agents. In certain embodiments, described herein are methods for the treatment of malaria by inhibition of plasmepsin X, IX and at least one other mechanism, comprising administration of combinations comprising an amount of at least one compound of Formula (I), or a pharmaceutically acceptable salt, solvate, ester or prodrug thereof, and an effective amount of one or more additional anti-malarial agents, wherein the additional anti-malarial agents act through a different mechanism than inhibiting plasmepsin IX or plasmepsin X. The pharmacological properties of the compounds of Formula (I), or a pharmaceutically acceptable salt thereof may be confirmed by several pharmacological assays. Certain assays are exemplified herein. 
     Dosage and Administration 
     Another embodiment provides suitable dosages and dosage forms of the compounds used in the methods of the invention. Suitable doses for administering compounds used in the methods of the invention to patients may readily be determined by those skilled in the art, e.g., by an attending physician, pharmacist, or other skilled worker, and may vary according to patient health, age, weight, frequency of administration, use with other active ingredients, and/or indication for which the compounds are administered. Doses may range from about 0.001 to 500 mg/kg of body weight/day of the compound of the invention. In one embodiment, the dosage is from about 0.01 to about 25 mg/kg of body weight/day of a compound of the invention, or a pharmaceutically acceptable salt or solvate of said compound. In another embodiment, the quantity of active compound in a unit dose of preparation may be varied or adjusted from about 1 mg to about 100 mg, in specific embodiments from about 1 mg to about 50 mg, in specific embodiments from about 1 mg to about 25 mg, according to the particular application. In another embodiment, a typical recommended daily dosage regimen for oral administration can range from about 1 mg/day to about 500 mg/day, in specific embodiments 1 mg/day to 200 mg/day, in two to four divided doses. 
     As discussed above, the amount and frequency of administration of the compounds of the invention and/or the pharmaceutically acceptable salts thereof will be regulated according to the judgment of the attending clinician considering such factors as age, condition and size of the patient as well as severity of the symptoms being treated. 
     Liquid form preparations include solutions, suspensions and emulsions. As an example, may be mentioned water or water-propylene glycol solutions for parenteral injection or addition of sweeteners and opacifiers for oral solutions, suspensions and emulsions. Liquid form preparations may also include solutions for intranasal administration. 
     Aerosol preparations suitable for inhalation may include solutions and solids in powder form, which may be in combination with a pharmaceutically acceptable carrier, such as an inert compressed gas, e.g. nitrogen. 
     Also included are solid form preparations that are intended to be converted, shortly before use, to liquid form preparations for either oral or parenteral administration. Such liquid forms include solutions, suspensions and emulsions. 
     Another embodiment provides for use of compositions comprising a compound of Formula (I), or a pharmaceutically acceptable salt thereof formulated for transdermal delivery. The transdermal compositions can take the form of creams, lotions, aerosols and/or emulsions and can be included in a transdermal patch of the matrix or reservoir type as are conventional in the art for this purpose. 
     Another embodiment provides for use of compositions comprising a compound of Formula (I), or a pharmaceutically acceptable salt thereof formulated for subcutaneous delivery. Another embodiment provides for use of compositions suitable for oral delivery. In some embodiments, it may be advantageous for the pharmaceutical preparation comprising one or more compounds of Formula (I), or a pharmaceutically acceptable salt thereof to be prepared in a unit dosage form. In such forms, the preparation is subdivided into suitably sized unit doses containing appropriate quantities of the active component, e.g., an effective amount to achieve the desired purpose. Each of the foregoing alternatives is considered as included in the various embodiments of the invention. 
     When used in combination with one or more additional therapeutic agents (“combination therapy”), the compounds used in the methods of this invention, i.e., the compounds of Formula (I), may be administered together or sequentially. When administered sequentially, compounds of the invention may be administered before or after the one or more additional therapeutic agents, as determined by those skilled in the art or patient preference. 
     If formulated as a fixed dose, such combination products employ the compounds of Formula (I), or a pharmaceutically acceptable salt thereof within the dosage range described herein and the other pharmaceutically active agent or treatment within its dosage range. 
     Combination Therapy 
     Another embodiment provides for methods of treatment using pharmaceutically acceptable compositions comprising a compound of the invention, either as the neat chemical or optionally further comprising additional ingredients. Such compositions are contemplated for preparation and use alone or in combination therapy. For preparing pharmaceutical compositions from the compounds of the invention, inert, pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include powders, tablets, dispersible granules, capsules, cachets and suppositories. The powders and tablets may be comprised of from about 5 to about 95 percent active ingredient. Suitable solid carriers are known in the art, e.g., magnesium carbonate, magnesium stearate, talc, sugar or lactose. Tablets, powders, cachets and capsules can be used as solid dosage forms suitable for oral administration. Examples of pharmaceutically acceptable carriers and methods of manufacture for various compositions may be found in A. Gennaro (ed.),  Remington&#39;s Pharmaceutical Sciences,  18 th  Edition, (1990), Mack Publishing Co., Easton, Pa. 
     Non-limiting examples of additional drugs and active agents useful in combination therapies for the treatment of malaria, include the following: Coartem® (Novartis International AG, Basel, Switzerland; artemether+lumefantrine), Eurartesim® (Sigma-Tau Pharmaceuticals, Inc., Rome, Italy; dihydroartemisinin-piperaquine), Pyramax® (Shin Poong Pharmaceutical Co., Ltd., Seoul, Korea; pyronaridine-artesunate), ASAQ Winthrop® (Sanofi SA (Gentilly, France)/DNDi (Geneva, Switzerland); artesunate+amodiaquine), ASMQ (Cipla Limited (Mumbai, India)/DNDi, artesunate+mefloquine), SPAQ-CO™ (Guilin Pharmaceutical Co., Ltd. (Shanghai), amodiaquine+sulfadoxine, pyrimethamine), Artesun® (Guilin Pharmaceutical, artesunate), artemether, artesunate, dihydroartemisinin, lumefantrine, amodiaquine, mefloquine, piperaquine, quinine, chloroquine, atovaquone and proguanil and sulfadoxine-pyrimethamine, Tafenoquine (Glaxosmithkline), OZ439/PQP (Sanofi), OZ439/FQ (Sanofi), KAE609 (Novartis), KAF156 (Novartis), DSM265 (NIH/Takeda), and MK-4815 (Merck &amp; Co., Inc., Powles et al.,  Antimicrobial Agents and Chemotherapy  56(5): 2414-2419(2012)). Selection of such additional active ingredients will be according to the diseases or disorders present for which treatment is desired, as determined by the attending physician or other health care provider. 
     Thus, the invention also provides methods of using the compounds of Formula (I), or a pharmaceutically acceptable salt thereof to inhibit plasmepsin X, plasmepsin IX or plasmepsin X and IX, to treat  Plasmodium  infection or treat malaria wherein the method further comprises administering to a subject in need thereof, one or more additional anti-malarial agents. In some embodiments, the one or more additional anti-malarial agents are selected from the group consisting of: artemether, lumefantrine, dihydroartemisinin, piperaquine, pyronaridine, artesunate, amodiaquine, mefloquine, sulfadoxine, pyrimethamine, lumefantrine, quinine, chloroquine, atovaquone, and proguanil. 
     Combinations can be administered concurrently or sequentially. Additionally, combinations can be administered in a single dose or multiple doses. 
     EXAMPLES 
     ACN=acetonitrile
 
AcOEt=ethylacetate
 
DCM=dichloromethane
 
DIAD=Diisopropyl azodicarboxylate
 
DIEA=N, N-Diisopropylethylamine, or Hunig&#39;s base
 
     DMF=N,N-Dimethylformamide 
     EDIC=EDC=1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
 
EtOAc=ethyl acetate
 
h=hrs.=hours
 
IPA=isopropyl alcohol
 
Hep=heptane
 
HOBt=hydroxybenzotriazole
 
K 2 CO 3 =potassium carbonate
 
LCMS=Liquid chromatography-mass spectrometry
 
LHMDS=LiHMDS=lithium bis(trimethylsilyl)amide
 
LiAlH 4 =lithium aluminum hydride
 
min=minutes
 
Me=methyl
 
MeOH═CH 3 OH=methanol
 
N 2 =nitrogen
 
NaBH 4 =sodium borohydride
 
Na 2 SO 4 =sodium sulfate
 
NH 4 Cl=Ammonium chloride
 
Pd(dppf)Cl 2 =[1,1′-Bis(diphenylphosphino)ferrocene]palladium(II) dichloride
 
     SFC=Supercritical Fluid Chromatography 
     SiO 2 =silicon dioxide
 
TFA=trifluoroacetic acid
 
THF=tetrahydrofuran
 
     TMS=Trimethylsilyl 
     CDCl 3 =heavy chloroform
 
CD 3 OD=heavy methanol
 
1 Standard atmosphere [atm]=101325 pascal [Pa]=14.6959488 psi
 
     The meanings of the abbreviations in the nuclear magnetic resonance spectra are shown below: s=singlet, d=doublet, dd=double doublet, dt=double triplet, ddd=double double doublet, sept=septet, t=triplet, m=multiplet, br=broad, brs=broad singlet, q=quartet, J=coupling constant and Hz=hertz. 
     Methods for preparing the compounds of this disclosure are described in the following Scheme and Examples. Starting materials and intermediates were purchased commercially from common catalog sources or were made using known procedures, or as otherwise illustrated. Some frequently applied routes to the compounds of Formula I are described in in the Schemes that follow. In some cases, the order of carrying out the reaction steps in the schemes may be varied to facilitate the reaction or to avoid unwanted reaction products. An asterisk (*) may be used in a chemical structure drawing that indicates the location of a chiral center. 
     
       
         
         
             
             
         
       
     
     Intermediate compounds of Formula S-3 were prepared by addition of organometallic reagents from halides S-1 onto aldehydes S-2. Alcohol mixtures S-3 were separated by SFC and the resulting chiral alcohols S-4 were subjected to Mitsunobu or nucleophilic substitution conditions with Boc-protected iminopyrimidones S-5 to give intermediates S-6. The ester intermediates S-6 were hydrolyzed to acids S-7. Finally intermediates S-7 were coupled with amines S-8 to provide final products of Formula S-9 after iminopyrimidone deprotection conditions using acids such as TFA or Zn. 
     Reactions sensitive to moisture or air were performed inside a glove-box or under nitrogen or argon using anhydrous solvents and reagents. The progress of reactions was determined by either analytical thin layer chromatography (TLC) usually performed with pre-coated TLC plates, silica gel 60F-254, layer thickness 0.25 mm or liquid chromatography-mass spectrometry (LC/MS). 
     Typically, the analytical LC-MS system used consisted of a Waters ZQ™ platform with electrospray ionization in positive ion detection mode with an Agilent 1100 series HPLC with autosampler. The column was a Waters Xterra MS C18, 3.0×50 mm, 5 μm or a Waters Acquity UPLC® BEH C18 1.0×50 mm, 1.7 μm. The flow rate was 1 mL/min, and the injection volume was 10 μL. UV detection was in the range 210-400 nm. The mobile phase consisted of solvent A (water plus 0.05% TFA) and solvent B (MeCN plus 0.05% TFA) with a gradient of 100% solvent A for 0.7 min changing to 100% solvent B over 3.75 min, maintained for 1.1 min, then reverting to 100% solvent A over 0.2 min. 
     Preparative HPLC purifications were usually performed using either a mass spectrometry directed system or a non-mass guided system. Usually they were performed on a Waters Chromatography Workstation configured with LC-MS System consisting of: Waters ZQ™ single quad MS system with Electrospray Ionization, Waters 2525 Gradient Pump, Waters 2767 Injecto/Collector, Waters 996 PDA Detector, the MS Conditions of: 150-750 amu, Positive Electrospray, Collection Triggered by MS, and a Waters SUNFIRE® C-18 5-micron, 30 mm (id)×100 mm column. The mobile phases consisted of mixtures of acetonitrile (10-100%) in water containing 0.1% TFA. Flow rates were maintained at 50 mL/min, the injection volume was 1800 μL, and the UV detection range was 210-400 nm. An alternate preparative HPLC system used was a Gilson Workstation consisting of: Gilson GX-281 Injector/Collector, Gilson UV/VIS-155 Detector, Gilson 333 and 334 Pumps, and either a Phenomenex Gemini-NX C-18 5-micron, 50 mm (id)×250 mm column or a Waters XBridge™ C-18 5-micron OBD™, 30 mm (id)×250 mm column. The mobile phases consisted of mixtures of acetonitrile (0-75%) in water containing 5 mmol (NH 4 )HCO 3 . Flow rates were maintained at 50 mL/min for the Waters Xbridge™ column and 90 mL/min for the Phenomenex Gemini column. The injection volume ranged from 1000-8000 μL, and the UV detection range was 210-400 nm. Mobile phase gradients were optimized for the individual compounds. Reactions performed using microwave irradiation were normally carried out using an Emrys Optimizer manufactured by Personal Chemistry, or an Initiator manufactured by Biotage. Concentration of solutions was carried out on a rotary evaporator under reduced pressure. Flash chromatography was usually performed using either a Biotage® Flash Chromatography apparatus (Dyax Corp.), an ISCO CombiFlash® Rf apparatus, or an ISCO CombiFlash® Companion XL on silica gel (32-63 μM, 60 Å pore size) in pre-packed cartridges of the size noted.  1 H NMR spectra were acquired at 500 MHz spectrometers in CDCl 3  solutions unless otherwise noted. Chemical shifts were reported in parts per million (ppm). Tetramethylsilane (TMS) was used as internal reference in CDCl 3  solutions, and residual CH 3 OH peak or TMS was used as internal reference in CD 3 OD solutions. Coupling constants (J) were reported in hertz (Hz). Chiral analytical chromatography was most commonly performed on one of CHIRALPAK® AS, CHIRALPAK® AD, CHIRALCEL© OD, CHIRALCEL©IA, or CHIRALCEL© OJ columns (250×4.6 mm) (Daicel Chemical Industries, Ltd.) with noted percentage of either ethanol in hexane (% Et/Hex) or isopropanol in heptane (% IPA/Hep) as isocratic solvent systems. Chiral preparative chromatography was conducted on one of CHIRALPAK AS, of CHIRALPAK AD, CHIRALCEL©OD, CHIRALCEL©IA, CHIRALCEL© OJ columns (20×250 mm) (Daicel Chemical Industries, Ltd.) with desired isocratic solvent systems identified on chiral analytical chromatography or by supercritical fluid (SFC) conditions. 
     It is understood that a chiral center in a compound may exist in the “S” or “R” stereo-configuration, or as a mixture of both. Within a molecule, each bond drawn as a straight line from a chiral center includes both the (R) and (S) stereoisomers as well as mixtures thereof. 
     Example 1 
     
       
         
         
             
             
         
       
     
     Step 1: 
     
       
         
         
             
             
         
       
     
     NaBH 4  (5.19 g, 137 mmol) was added to a solution of methyl 3-formylbenzoate (15 g, 91 mmol) in MeOH (150 mL), stirring at −40° C. After addition, the mixture was stirred at −40° C. for 20 min. The mixture was quenched with water (30 mL), and extracted with EtOAc (50 mL*3). The organic layers were washed with brine (60 mL), dried over anhydrous Na 2 SO 4 , filtered and concentrated. The residue was purified by column chromatography (SiO 2 , Pet. ether/EtOAc=20:1-5:1) to afford product methyl 3-(hydroxymethyl)benzoate. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ=8.05 (s, 1H), 7.98 (d, J=7.6 Hz, 1H), 7.59 (d, J=7.6 Hz, 1H), 7.50-7.41 (m, 1H), 4.77 (br s, 2H), 3.93 (s, 3H). 
     Step 2: 
     
       
         
         
             
             
         
       
     
     (E)-diisopropyl diazene-1,2-dicarboxylate (30.8 mL, 156 mmol) was added dropwise to a solution of tert-butyl (4,4-diethyl-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate (21.07 g, 78 mmol), methyl 3-(hydroxymethyl)benzoate (13 g, 78 mmol) and triphenylphosphine (41.0 g, 156 mmol) in THF (200 mL) at 0° C. under N 2  atmosphere. The mixture was stirred at 15° C. for 16 hours and then concentrated in vacuo. The residue was purified by Prep-TLC (Pet. ether/EtOAc=1:1) to afford methyl 3-((2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)methyl)benzoate. 
     MS (ESI) m z: 418.3 (M+H + ) 
       1 H NMR (500 MHz, CHLOROFORM-d) δ=9.85 (s, 1H), 8.08 (s, 1H), 7.95-7.89 (m, 1H), 7.61 (d, J=7.5 Hz, 1H), 7.37 (t, J=8.0 Hz, 1H), 5.20 (s, 2H), 3.91 (s, 3H), 2.62 (s, 2H), 1.61-1.56 (m, 4H), 1.51 (s, 9H), 0.89 (t, J=7.5 Hz, 6H). 
     
       
         
         
             
             
         
       
     
     Step 3: 
     To a solution of methyl 3-((2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)methyl)benzoate (3.5 g, 8.38 mmol) in THF (80 mL) was added potassium trimethylsilanolate (3.23 g, 25.1 mmol). The mixture was stirred at 25° C. for 2 hrs. TLC and LCMS showed no SM. The mixture was used for next step directly without purification. 
     MS (ESI) m z: 404.2 (M+H + ) 
     Step 4: 
     
       
         
         
             
             
         
       
     
     DIEA (5.85 mL, 33.5 mmol) was added to a solution of 3-((2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)methyl)benzoic acid (3.38 g, 8.38 mmol), EDCI (3.21 g, 16.75 mmol), HOBt (2.264 g, 16.75 mmol) and (3S,4R)-4-amino-2,2-dimethylchroman-3-ol (2.104 g, 10.89 mmol) in THF (80 mL). The reaction was stirred at 30° C. for 16 h. The mixture was quenched with water (20 mL), and extracted with EtOAc (20 mL*3). The organic layers were washed with brine (30 mL), dried over Na 2 SO 4 , filtered, and concentrated in vacuo to afford crude product which was purified by column chromatography (SiO 2 , Pet.ether:EtOAc=5:1 to 0:1) tert-butyl (4,4-diethyl-1-(3-(((3S,4R)-3-hydroxy-2,2-dimethylchroman-4-yl)carbamoyl)benzyl)-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate. 
     MS (ESI) m z: 579.3 (M+H + ) 
       1 H NMR (400 MHz, CHLOROFORM-d) δ 9.82 (s, 1H), 7.85 (d, J=1.2 Hz, 1H), 7.73 (dd, J=1.2, 7.6 Hz, 1H), 7.56-7.63 (m, 1H), 7.35-7.44 (m, 1H), 7.27 (d, J=8.0 Hz, 1H), 7.21 (t, J=7.6 Hz, 1H), 6.91-7.00 (m, 1H), 6.91-7.00 (m, 1H), 6.82-6.89 (m, 1H), 6.46 (d, J=7.2 Hz, 1H), 5.22 (t, J=8.0 Hz, 1H), 5.15 (s, 2H), 4.51 (dd, J=1.2, 2.4 Hz, 1H), 3.73-3.80 (m, 1H), 2.58 (s, 2H), 1.51-1.57 (m, 4H), 1.49 (s, 3H), 1.42 (d, J=1.2 Hz, 9H), 1.29 (s, 3H), 0.83-0.90 (m, 6H). 
     Step 5: 
     
       
         
         
             
             
         
       
     
     A solution of tert-butyl (4,4-diethyl-1-(3-(((3S,4R)-3-hydroxy-2,2-dimethylchroman-4-yl)carbamoyl)benzyl)-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate (4 g, 6.91 mmol) in 4N HCl-dioxane (80 mL) was stirred at 15° C. for 24 h. The mixture was concentrated and purified by HPLC (Instrument CL; Method Column YMC-Actus Triart C18 150*30 mm*5 um; Condition water (0.05% HCl)-ACN Begin B 17; End B 57 Gradient Time (min) 10; 100% B Hold Time (min) 2 FlowRate (mL/min) 30; Injections 30) and (Instrument eb; Method Column Phenomenex Synergi C18 150*30 mm*4 um; Condition water (0.05% HCl)-ACN Begin B 22; End B 42 Gradient Time (min) 10; 100% B Hold Time (min) 2 FlowRate (mL/min) 25; Injections 30) to afford 3-((4,4-diethyl-2-imino-6-oxotetrahydropyrimidin-1(2H)-yl)methyl)-N-((3S,4R)-3-hydroxy-2,2-dimethylchroman-4-yl)benzamide (Example 1). 
     MS (ESI) m z: 479.3 (M+H + ) 
       1 H NMR (500 MHz, METHANOL-d 4 ) δ 7.84-7.88 (m, 2H), 7.50 (d, J=5.0 Hz, 2H), 7.11-7.16 (m, 2H), 6.84-6.89 (m, 1H), 6.77 (d, J=8.0 Hz, 1H), 5.26 (d, J=10.0 Hz, 1H), 5.18 (s, 2H), 3.82 (d, J=10.0 Hz, 1H), 2.90-2.97 (m, 2H), 1.65-1.74 (m, 4H), 1.46-1.52 (m, 3H), 1.25-1.30 (m, 3H), 0.90-0.98 (m, 6H). 
     Example 2 
     
       
         
         
             
             
         
       
     
     Step 1: 
     
       
         
         
             
             
         
       
     
     Isopropylmagnesium lithium chloride (68.1 mL, 89 mmol) was added dropwise to a solution of methyl 3-iodobenzoate (23.20 g, 89 mmol) in THF (200 mL) at −40° C. under N 2  atmosphere. The mixture was stirred at −40° C. for 1 hour. To the mixture, 3-methoxypropanal (6.5 g, 73.8 mmol) in THF (20 mL) was added dropwise. The mixture was stirred at −40° C. for another 1 h. The mixture was quenched with saturated aqueous NH 4 Cl (100 mL), and extracted with EtOAc (300 mL*3). The combined organic layers were washed with brine (100 mL), dried over Na 2 SO 4 , filtered, concentrated in vacuo. The residue was purified by flash column (Pet. ether/EtOAc=10:1 to 5:1) to afford methyl 3-(1-hydroxy-3-methoxypropyl)benzoate. 
     Step 2: 
     
       
         
         
             
             
         
       
     
     Methyl 3-(1-hydroxy-3-methoxypropyl)benzoate (12.8 g, 57.1 mmol) was separated by supercritical fluid chromatography (SFC) (Instrument SFC-9 Method Column DAICEL CHIRALPAK AD (250 mm*50 mm, 10 um) Condition 0.1% NH 3 H 2 O ETOH Begin B 25% End B 25% Gradient Time (min) 100% B Hold Time (min) FlowRate (mL/min) 200 Injections 280) to afford product (R)-methyl 3-(1-hydroxy-3-methoxypropyl)benzoate (p1, Rt=2.928) and (S)-methyl 3-(1-hydroxy-3-methoxypropyl)benzoate (p2, Rt=3.178). 
     3A:  1 H NMR (500 MHz, CHLOROFORM-d) δ 8.04 (s, 1H), 7.93-7.95 (m, 1H), 7.60 (d, J=8.0 Hz, 1H), 7.43 (t, J=8.0 Hz, 1H), 4.94-5.04 (m, 1H), 3.92 (s, 3H), 3.58-3.63 (m, 2H), 3.55 (d, J=3.0 Hz, 1H), 3.39 (s, 3H), 1.91-2.19 (m, 2H). 
     3B:  1 H NMR (500 MHz, CHLOROFORM-d) δ 8.03 (s, 1H), 7.89-8.00 (m, 1H), 7.60 (d, J=7.5 Hz, 1H), 7.43 (t, J=7.5 Hz, 1H), 4.89-5.05 (m, 1H), 3.92 (s, 3H), 3.58-3.66 (m, 2H), 3.49-3.57 (m, 1H), 3.39 (s, 3H), 1.94-2.09 (m, 2H). 
     Step 3: 
     
       
         
         
             
             
         
       
     
     4-nitrobenzoic acid (2.98 g, 17.84 mmol), triphenylphosphine (7.02 g, 26.8 mmol) and (E)-diisopropyl diazene-1,2-dicarboxylate (7.21 g, 35.7 mmol) were added to a solution of (R)-methyl 3-(1-hydroxy-3-methoxypropyl)benzoate (4 g, 17.84 mmol) in THF (40 mL) at 15° C. under N 2  atmosphere. The mixture was stirred at 15° C. for 16 h. The mixture was cooled, and solvent was evaporated under reduced pressure to give the crude product. The crude product was purified by flash silica gel chromatography (ISCO®; 120 g SepaFlash® Silica Flash Column, Eluent of 30% EA/PE gradient at 40 mL/min) to give (S)-methyl 3-(3-methoxy-1-((4-nitrobenzoyl)oxy)propyl)benzoate. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ 8.25-8.30 (m, 2H), 8.18-8.24 (m, 2H), 8.09 (s, 1H), 7.98 (d, J=7.2 Hz, 1H), 7.61 (d, J=7.6 Hz, 1H), 7.44 (t, J=7.6 Hz, 1H), 6.16-6.20 (m, 1H), 3.90 (s, 3H), 3.43-3.52 (m, 1H), 3.34-3.36 (m, 1H), 3.29 (s, 3H), 2.33-2.47 (m, 1H), 2.08-2.22 (m, 1H). 
     Step 4: 
     
       
         
         
             
             
         
       
     
     K 2 CO 3  (8.33 g, 60.3 mmol) was added to a solution of (S)-methyl 3-(3-methoxy-1-((4-nitrobenzoyl)oxy)propyl)benzoate (7.5 g, 20.09 mmol) in MeOH (70 mL) at 15° C. under N 2  atmosphere. The mixture was stirred at 15° C. for 3 h. The mixture was cooled, diluted with water (50 mL), extracted with EtOAc (3×50 mL), dried over Na 2 SO 4 , filtered and the solvent was evaporated under reduced pressure to give the crude product. The crude product was purified by flash silica gel chromatography (ISCO®; 40 g SepaFlash® Silica Flash Column, Eluent of 10% EA/PE gradient at 40 mL/min) to give (S)-methyl 3-(1-hydroxy-3-methoxypropyl)benzoate.  1 H NMR (400 MHz, CHLOROFORM-d) δ 8.03 (s, 1H), 7.94 (d, J=8.0 Hz, 1H), 7.59 (d, J=8.0 Hz, 1H), 7.39-7.47 (m, 1H), 4.96-4.99 (m, 1H), 3.92 (s, 3H), 3.57-3.61 (m, 2H), 3.38 (s, 3H), 1.94-2.05 (m, 2H). 
     Step 5: 
     
       
         
         
             
             
         
       
     
     (E)-diisopropyl diazene-1,2-dicarboxylate (2.64 mL, 13.38 mmol) was added dropwise to a solution of tert-butyl (4,4-diethyl-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate (1.982 g, 7.36 mmol), (R)-methyl 3-(1-hydroxy-3-methoxypropyl)benzoate (1.5 g, 6.69 mmol) and triphenylphosphine (3.51 g, 13.38 mmol) in THF (25 mL) at 0° C. under N 2  atmosphere. Then the mixture was stirred at 20° C. for 16 h. The mixture was concentrated in vacuo and purified by Prep-TLC (Pet. ether/EtOAc=2:1) to afford (R)-methyl 3-(1-(2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)benzoate. 
     MS (ESI) m z 476.2 (M+H + ) 
       1 H NMR (500 MHz, CHLOROFORM-d) δ 8.12 (s, 1H), 7.90 (d, J=8.0, 1H), 7.65 (d, J=7.5 Hz, 1H), 7.33-7.39 (m, 1H), 5.03-5.09 (m, 1H), 4.93-5.02 (m, 1H), 3.78-4.00 (m, 3H), 3.42-3.55 (m, 2H), 3.19-3.37 (m, 3H), 2.49-2.59 (m, 2H), 1.56-1.69 (m, 4H), 1.44-1.53 (m, 9H), 1.32 (d, J=6.0 Hz, 4H), 1.19-1.31 (m, 8H), 0.90 (td, J=7.5, 11.5 Hz, 6H). 
     Step 6: 
     
       
         
         
             
             
         
       
     
     Potassium trimethylsilanolate (2.217 g, 17.28 mmol) was added to a solution of (R)-methyl 3-(1-(2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)benzoate (2.74 g, 5.76 mmol) in THF (60 mL). The mixture was stirred at 25° C. for 1 h. The mixture was used for next step directly without purification. 
     MS (ESI) m z 462.3 (M+H + ) 
     
       
         
         
             
             
         
       
     
     Step 7: 
     DIEA (4.03 mL, 23.05 mmol) was added to a solution of (R)-3-(1-(2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)benzoic acid (2.66 g, 5.76 mmol), EDCI (2.210 g, 11.53 mmol), HOBt (1.557 g, 11.53 mmol) and (1R,2R)-1-amino-2,3-dihydro-1H-inden-2-ol (1.290 g, 8.64 mmol) in THF (80 mL). The reaction was stirred at 30° C. for 16 h. The mixture was quenched with water (30 mL), and extracted with EtOAc (50 mL*3). The organic layers were washed with brine (50 mL), dried over Na 2 SO 4 , filtered, and concentrated in vacuo to afford crude product which was purified by column chromatography (SiO 2 , Pet.ether:EtOAc=5:1 to 0:1) tert-butyl (4,4-diethyl-1-((R)-1-(3-(((1R,2R)-2-hydroxy-2,3-dihydro-1H-inden-1-yl)carbamoyl)phenyl)-3-methoxypropyl)-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate. 
     MS (ESI) m z 593.3 (M+H + ) 
       1 H NMR (400 MHz, CHLOROFORM-d) δ 9.90 (s, 1H), 7.94 (s, 1H), 7.63-7.70 (m, 1H), 7.39 (t, J=7.83 Hz, 1H), 7.30 (br s, 3H), 6.62 (br s, 1H), 6.42 (br s, 1H), 5.32 (t, J=6.0 Hz, 1H), 4.80 (s, 1H), 4.50-4.59 (m, 1H), 3.44-3.52 (m, 2H), 3.37 (dd, J=8.0, 16.0 Hz, 1H), 3.29 (s, 3H), 3.01 (dd, J=8.4, 16.0 Hz, 1H), 2.81 (s, 1H), 2.45-2.56 (m, 3H), 1.62 (br d, J=7.6 Hz, 2H), 1.52-1.56 (m, 2H), 1.46 (s, 9H), 0.86-0.92 (m, 6H). 
     Step 8: 
     
       
         
         
             
             
         
       
     
     A solution of tert-butyl (4,4-diethyl-1-((R)-1-(3-(((1R,2R)-2-hydroxy-2,3-dihydro-1H-inden-1-yl)carbamoyl)phenyl)-3-methoxypropyl)-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate (3 g, 5.06 mmol) in 4N HCl-dioxane (80 mL) was stirred at 15° C. for 24 h. The mixture was concentrated and purified by HPLC (Instrument CL Method Column YMC-Actus Triart C18 150*30 mm*5 um Condition water (0.05% HCl)-ACN Begin B 15 End B 55 Gradient Time (min) 10 100% B Hold Time (min) 2 FlowRate (mL/min) 30 Injections 14) to afford 3-((R)-1-(4,4-diethyl-2-imino-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)-N-((1R,2R)-2-hydroxy-2,3-dihydro-1H-inden-1-yl)benzamide (Example 2). 
     MS (ESI) m z 493.3 (M+H + ) 
       1 H NMR (500 MHz, METHANOL-d 4 ) δ 7.93 (s, 1H), 7.85 (br d, J=7.5 Hz, 1H), 7.60 (br d, J=7.5 Hz, 1H), 7.47-7.53 (m, 1H), 7.23 (br s, 2H), 7.17-7.22 (m, 2H), 5.62 (br s, 1H), 5.45 (d, J=6.5 Hz, 1H), 4.51 (q, J=7.0 Hz, 1H), 3.55-3.67 (m, 2H), 3.38 (s, 3H), 3.27-3.29 (m, 1H), 2.82-2.91 (m, 3H), 2.59-2.70 (m, 2H), 1.71-1.74 (m, 2H), 1.57 (q, J=7.5 Hz, 2H), 0.89-0.98 (in, 6H). 
     The following Examples were made according to the Scheme described above using appropriate starting materials. 
     
       
         
           
               
               
               
               
             
               
                   
               
               
                 Ex. 
                   
                   
                 LC/MS 
               
               
                 No. 
                 Structure 
                 Name 
                 (M + 1)+ 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                  3 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]methyl]-4- isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 435.1 
               
               
                   
               
               
                  4A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-phenyl- methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 511.1 
               
               
                   
               
               
                  4B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4R)-chroman-4- yl]carbamoyl]phenyl]-phenyl- methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 511 
               
               
                   
               
               
                  5A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4- (cyclopropylmethyl)-6-oxo-4- phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 527 
               
               
                   
               
               
                  5B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-1-[[3-[[(4R)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4- (cyclopropylmethyl)-6-oxo-4- phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 527 
               
               
                   
               
               
                  6A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]methyl]-4- (cyclopropylmethyl)-6-oxo-4- phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 509 
               
               
                   
               
               
                  6B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-1-[[3-[[(4R)-chroman-4- yl]carbamoyl]phenyl]methyl]-4- (cyclopropylmethyl)-6-oxo-4- phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 509 
               
               
                   
               
               
                  7 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]ethyl]-4- (cyclopropylmethyl)-6-oxo-4- phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 523.2 
               
               
                   
               
               
                  8 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-pyridin-1- ium-3-yl-methyl]-4- (cyclopropylmethyl)-6-oxo-4- phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 586.2 
               
               
                   
               
               
                  9A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[(R)-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-phenyl- methyl]-4-isopropyl-4-methyl-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 511 
               
               
                   
               
               
                  9B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[(R)-[3-[[(4R)-chroman-4- yl]carbamoyl]phenyl]-phenyl- methyl]-4-isopropyl-4-methyl-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 511.1 
               
               
                   
               
               
                  10 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-4- isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 435.1 
               
               
                   
               
               
                  11 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 435 
               
               
                   
               
               
                  12 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 453 
               
               
                   
               
               
                  13A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]butyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 477.1 
               
               
                   
               
               
                  13B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]butyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 477.1 
               
               
                   
               
               
                  14A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-3-methyl- butyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 491.1 
               
               
                   
               
               
                  14B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-3-methyl- butyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 491.2 
               
               
                   
               
               
                  15 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 435.1 
               
               
                   
               
               
                  16 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-phenyl- methyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 511.1 
               
               
                   
               
               
                  17 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-4-(cyclopropylmethyl)-1-[[3- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-6- oxo-4-phenyl-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 509 
               
               
                   
               
               
                  18 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[[(1R,2S)-2- hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-4- isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 435.1 
               
               
                   
               
               
                  19 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-2-methoxy- ethyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 479.1 
               
               
                   
               
               
                  20 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-2-fluoro- phenyl]methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 453 
               
               
                   
               
               
                  21 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-4-fluoro- phenyl]methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 453.1 
               
               
                   
               
               
                  22 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[2-[[(4S)-chroman-4- yl]carbamoyl]-4-pyridyl]methyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 436.1 
               
               
                   
               
               
                  23 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[4-[[(4S)-chroman-4- yl]carbamoyl]-2-pyridyl]methyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 436.1 
               
               
                   
               
               
                  24 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[5-[[(4S)-chroman-4- yl]carbamoyl]-3-pyridyl]methyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 436.1 
               
               
                   
               
               
                  25 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]ethyl]-4- isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 449.2 
               
               
                   
               
               
                  26 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[1-bicyclo[1.1.1]pentanyl- [3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]methyl]-4- isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 501.2 
               
               
                   
               
               
                  27 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]ethyl]-4- isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 449.1 
               
               
                   
               
               
                  28 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[1-bicyclo[1.1.1]pentanyl- [3-[[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-4- isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 501.2 
               
               
                   
               
               
                  29 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4-isopropyl-4- methyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 453 
               
               
                   
               
               
                  30 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-fluoro-5-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-4- isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 453 
               
               
                   
               
               
                  31 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]ethyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 449.1 
               
               
                   
               
               
                  32 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-bicyclo[1.1.1]pentanyl-[3- [[(4S)-chroman-4- yl]carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 501.2 
               
               
                   
               
               
                  33 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]ethyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 449.2 
               
               
                   
               
               
                  34 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-bicyclo[1.1.1]pentanyl-[3- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 501.2 
               
               
                   
               
               
                  35 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-fluoro-5- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 453.2 
               
               
                   
               
               
                  36 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[2-[[(1R,2R)-2- hydroxyindan-1-yl]carbamoyl]-4- pyridyl]methyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 436.1 
               
               
                   
               
               
                  37 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[6-[[(1R,2R)-2- hydroxyindan-1-yl]carbamoyl]-2- pyridyl]methyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 436.1 
               
               
                   
               
               
                  38 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[4-[[(1R,2R)-2- hydroxyindan-1-yl]carbamoyl]-2- pyridyl]methyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 436.1 
               
               
                   
               
               
                  39 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[5-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]pyridin-1-ium-3- yl]methyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 436.1 
               
               
                   
               
               
                  40 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-2-phenyl- ethyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 525.1 
               
               
                   
               
               
                  41 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]but-3-enyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 475 
               
               
                   
               
               
                  42 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4,4-diethyl-5- methyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 467 
               
               
                   
               
               
                  43 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4,4-diethyl-5- methyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 467 
               
               
                   
               
               
                  44 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4-ethyl-4-isopropyl- 6-oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 467.1 
               
               
                   
               
               
                  45 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-4-ethyl-1-[[3-fluoro-5- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-4- isopropyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 467.1 
               
               
                   
               
               
                  46 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4-ethyl-4-isobutyl- 6-oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 481.1 
               
               
                   
               
               
                  47 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-4-ethyl-1-[[3-fluoro-5- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-4- isobutyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 481.1 
               
               
                   
               
               
                  48 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-fluoro-5- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-5- methyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 467 
               
               
                   
               
               
                  49A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[4-[[(4S)-chroman-4- yl]carbamoyl]pyridin-1-ium-2- yl]butyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 478.1 
               
               
                   
               
               
                  49B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[4-[[(4S)-chroman-4- yl]carbamoyl]pyridin-1-ium-2- yl]butyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 478.1 
               
               
                   
               
               
                  50 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[5-[[(4S)-chroman-4- yl]carbamoyl]pyridin-1-ium-3- yl]butyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 478.1 
               
               
                   
               
               
                  51A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[(2,2-dimethylchroman-4- yl)carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 463.2 
               
               
                   
               
               
                  51B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[(2,2-dimethylchroman-4- yl)carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 463.2 
               
               
                   
               
               
                  52 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4-ethyl-6-oxo-4- phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 501 
               
               
                   
               
               
                  53 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-4-ethyl-1-[[3-fluoro-5- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-6- oxo-4-phenyl-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 501.1 
               
               
                   
               
               
                  54 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-3-morpholin- 4-ium-4-yl-propyl]-4,4-diethyl-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 548.1 
               
               
                   
               
               
                  55 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-3-morpholin- 4-ium-4-yl-propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 548.2 
               
               
                   
               
               
                  56 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-3-methoxy- propyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 493.2 
               
               
                   
               
               
                  57 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[5-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]pyridin-1-ium-3- yl]butyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 478.1 
               
               
                   
               
               
                  58 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-3-pyrrolidin- 1-ium-1-yl-propyl]-4,4-diethyl-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 532.3 
               
               
                   
               
               
                  59 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-3-hydroxy- propyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 479.1 
               
               
                   
               
               
                  60 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4-ethyl-4-isopropyl- 6-oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 467 
               
               
                   
               
               
                  61 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-4-ethyl-1-[[3-fluoro-5- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-4- isopropyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 467 
               
               
                   
               
               
                  62A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[(2,2-dimethylchroman-4- yl)carbamoyl]phenyl]butyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 505.1 
               
               
                   
               
               
                  62B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[(2,2-dimethylchroman-4- yl)carbamoyl]phenyl]butyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 505.1 
               
               
                   
               
               
                  63 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-fluoro- phenyl]methyl]-4-ethyl-6-oxo-4- (trifluoromethyl)hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 493.2 
               
               
                   
               
               
                  64 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-1-[(1R)-1-[3-[[(4S)-chroman- 4-yl]carbamoyl]phenyl]butyl]-4- (cyclopropylmethyl)-6-oxo-4- phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 551.1 
               
               
                   
               
               
                  65 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-4-(cyclopropylmethyl)-1- [(1R)-1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]butyl]-6-oxo- 4-phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 551 
               
               
                   
               
               
                  66 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-1-[(1R)-1-[3-[[(4S)-chroman- 4-yl]carbamoyl]phenyl]butyl]-4- ethyl-6-oxo-4-phenyl- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 525.2 
               
               
                   
               
               
                  67 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4R)-4-ethyl-1-[(1R)-1-[3- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]butyl]-6-oxo- 4-phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 525.2 
               
               
                   
               
               
                  68 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[(1R)-1-[3-[[(4S)-chroman- 4-yl]carbamoyl]phenyl]butyl]-4- (cyclopropylmethyl)-6-oxo-4- phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 551.3 
               
               
                   
               
               
                  69 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-4-(cyclopropylmethyl)-1- [(1R)-1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]butyl]-6-oxo- 4-phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 551.2 
               
               
                   
               
               
                  70 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[(1R)-1-[3-[[(4S)-chroman- 4-yl]carbamoyl]phenyl]butyl]-4- ethyl-6-oxo-4-phenyl- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 525.2 
               
               
                   
               
               
                  71 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-4-ethyl-1-[(1R)-1-[3- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]butyl]-6-oxo- 4-phenyl-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 525.3 
               
               
                   
               
               
                  72A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[(2,2-dimethylchroman-4- yl)carbamoyl]phenyl]-3-methoxy- propyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 521.1 
               
               
                   
               
               
                  72B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[(2,2-dimethylchroman-4- yl)carbamoyl]phenyl]-3-methoxy- propyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 521.1 
               
               
                   
               
               
                  73A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-3-cyano- propyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 488.3 
               
               
                   
               
               
                  74 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]hex-4-ynyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 501.2 
               
               
                   
               
               
                  75 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[[(1S)-indan-1- yl]carbamoyl]phenyl]methyl]-4- isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 419.2 
               
               
                   
               
               
                  76 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[[(1S)-indan-1- yl]carbamoyl]phenyl]butyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 461.3 
               
               
                   
               
               
                  77 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[[(1S)-indan-1- yl]carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 419.3 
               
               
                   
               
               
                  78 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-2-methyl- buta-2,3-dienyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 487.2 
               
               
                   
               
               
                  79 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[(2,2-dimethylchroman- 4-yl)carbamoyl]phenyl]methyl]-4- isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 463.2 
               
               
                   
               
               
                  80 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[4-[(2,2-dimethylchroman-4- yl)carbamoyl]pyridin-1-ium-2- yl]methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 464 
               
               
                   
               
               
                  81 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[5-[(2,2-dimethylchroman-4- yl)carbamoyl]pyridin-1-ium-3- yl]methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 464.2 
               
               
                   
               
               
                  82 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[5-[(2,2-dimethylchroman-4- yl)carbamoyl]pyridin-1-ium-3- yl]methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 464.2 
               
               
                   
               
               
                  83A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[(2,2-dimethylchroman-4- yl)carbamoyl]phenyl]hex-4-ynyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 529.3 
               
               
                   
               
               
                  83B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[(2,2-dimethylchroman-4- yl)carbamoyl]phenyl]hex-4-ynyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 529.3 
               
               
                   
               
               
                  83C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[(2,2-dimethylchroman-4- yl)carbamoyl]phenyl]hex-4-ynyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 529.4 
               
               
                   
               
               
                  84 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]-5-cyano- phenyl]methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 460.3 
               
               
                   
               
               
                  85A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-[(4S)-chroman-4-yl]-3-[(4,4- diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-thiazol-5- yl-methyl]benzamide 
                 518.2 
               
               
                   
               
               
                  85B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-thiazol-5-yl- methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 518.2 
               
               
                   
               
               
                  86 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]methyl]-4- ethyl-4-(methoxymethyl)-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 451.3 
               
               
                   
               
               
                  87 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[(3- fluorochroman-4- yl)carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 453.2 
               
               
                   
               
               
                  88 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[(6-fluoro-2,2- dimethyl-chroman-4- yl)carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 481.2 
               
               
                   
               
               
                  89A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-cyano-5-[(2,2- dimethylchroman-4- yl)carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 488.3 
               
               
                   
               
               
                  89B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-cyano-5-[(2,2- dimethylchroman-4- yl)carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 488.2 
               
               
                   
               
               
                  90A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-thiazol-5- yl-methyl]-N-(2,2- dimethylchroman-4-yl)benzamide 
                 546.2 
               
               
                   
               
               
                  90B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-thiazol-5- yl-methyl]-N-(2,2- dimethylchroman-4-yl)benzamide 
                 546.2 
               
               
                   
               
               
                  90C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-thiazol-5- yl-methyl]-N-(2,2- dimethylchroman-4-yl)benzamide 
                 546.2 
               
               
                   
               
               
                  91A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[[3-[[2- (trifluoromethyl)chroman-4- yl]carbamoyl]phenyl]methyl]hexa- hydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 503.2 
               
               
                   
               
               
                  91B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[[3-[[2- (trifluoromethyl)chroman-4- yl]carbamoyl]phenyl]methyl]hexa- hydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 503.2 
               
               
                   
               
               
                  92A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)- pyrimidin-5-yl-methyl]-N-(2,2- dimethylchroman-4-yl)benzamide 
                 541.2 
               
               
                   
               
               
                  92B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)- pyrimidin-5-yl-methyl]-N-(2,2- dimethylchroman-4-yl)benzamide 
                 541.1 
               
               
                   
               
               
                  92C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)- pyrimidin-5-yl-methyl]-N-(2,2- dimethylchroman-4-yl)benzamide 
                 541.3 
               
               
                   
               
               
                  93 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[(7-fluoro-2,2- dimethyl-chroman-4- yl)carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 481.2 
               
               
                   
               
               
                  94A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[(5-fluoro-2,2- dimethyl-chroman-4- yl)carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 481.3 
               
               
                   
               
               
                  94B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[(5-fluoro-2,2- dimethyl-chroman-4- yl)carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 481.3 
               
               
                   
               
               
                  95 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4-ethyl-1-[[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]methyl]-4- (methoxymethyl)-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 451.3 
               
               
                   
               
               
                  96 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]phenyl]-2-pyridin-1- ium-3-yl-propyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidenelammonium; 2,2,2- trifluoroacetate 
                 568.2 
               
               
                   
               
               
                  97 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[[3-[[2- (trifluoromethyl)chroman-4- yl]carbamoyl]phenyl]methyl]hexa- hydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 503.2 
               
               
                   
               
               
                  98A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]phenyl]methyl]-4- ethyl-4-(methoxymethyl)-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 479.3 
               
               
                   
               
               
                  98B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]phenyl]methyl]-4- ethyl-4-(methoxymethyl)-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 479.3 
               
               
                   
               
               
                  99A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]phenyl]-(5- fluoropyridin-1-ium-3-yl)methyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 558.3 
               
               
                   
               
               
                  99B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]phenyl]-(5- fluoropyridin-1-ium-3-yl)methyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 558.3 
               
               
                   
               
               
                 100A  
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]phenyl]-pyridin-1- ium-3-yl-methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 540.3 
               
               
                   
               
               
                 100B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]phenyl]-pyridin-1- ium-3-yl-methyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 540.3 
               
               
                   
               
               
                 101A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]phenyl]-pyridin-1- ium-3-yl-methyl]-4-isopropyl-4- methyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 540.3 
               
               
                   
               
               
                 101B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[[3-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]phenyl]-pyridin-1- ium-3-yl-methyl]-4-isopropyl-4- methyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 540.3 
               
               
                   
               
               
                 102 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[(1R,2R)- 2-hydroxyindan-1- yl]carbamoyl]phenyl]butyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 477.3 
               
               
                   
               
               
                 103 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[5-I(4S)-2,2- dimethylchroman-4- yl]carbamoyl]pyridin-1-ium-3-yl]- 3-methoxy-propyl]-4,4-diethyl-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 522.4 
               
               
                   
               
               
                 104 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[1-[5-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]pyridin-1-ium-3-yl]- 3-methoxy-propyl]-4-isopropyl-4- methyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 522.4 
               
               
                   
               
               
                 105A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[5-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]pyridin-1-ium-3- yl]-thiazol-5-yl-methyl]-4,4-diethyl- 6-oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 547.3 
               
               
                   
               
               
                 105B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[5-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]pyridin-1-ium-3- yl]-thiazol-5-yl-methyl]-4,4-diethyl- 6-oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 547.3 
               
               
                   
               
               
                 106A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-(5- fluoropyridin-1-ium-3-yl)methyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 530.2 
               
               
                   
               
               
                 106B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-(5- fluoropyridin-1-ium-3-yl)methyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 530.2 
               
               
                   
               
               
                 107 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[3-cyano-1-[3-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]phenyl]propyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 516.2 
               
               
                   
               
               
                 108A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[1-[3-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]phenyl]pent-4-ynyl]- 4-isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 515.3 
               
               
                   
               
               
                 108B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[1-[3-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]phenyl]pent-4-ynyl]- 4-isopropyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 515.3 
               
               
                   
               
               
                 109A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-pyridin-1- ium-3-yl-methyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 512.3 
               
               
                   
               
               
                 109B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-pyridin-1- ium-3-yl-methyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 512.3 
               
               
                   
               
               
                 110A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4,-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)- pyrimidin-5-yl-methyl]-N-[(1R,2R)- 2-hydroxyindan-1-yl]benzamide 
                 513.3 
               
               
                   
               
               
                 110B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)- pyrimidin-5-yl-methyl]-N-[(1R,2R)- 2-hydroxyindan-1-yl]benzamide 
                 513.2 
               
               
                   
               
               
                 111 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[5-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]pyridin-1-ium-3- yl]hex-4-ynyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 530.3 
               
               
                   
               
               
                 112A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-2,2- dimethylchroman-4- 6-oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 551.3 
               
               
                   
               
               
                 112B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]phenyl]-2-(2- methoxyethoxy)ethyl]-4,4-diethyl- 6-oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 551.3 
               
               
                   
               
               
                 113 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)methyl]- N-(4-methylchroman-4- yl)benzamide 
                 449.2 
               
               
                   
               
               
                 114A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[1-[5-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]pyridin-1-ium-3- yl]pent-4-ynyl]-4-isopropyl-4- methyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 516.2 
               
               
                   
               
               
                 114B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[1-[5-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]pyridin-1-ium-3- yl]pent-4-ynyl]-4-isopropyl-4- methyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 516.2 
               
               
                   
               
               
                 115A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]phenyl]-(4- methylsulfonylphenyl)methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 617.2 
               
               
                   
               
               
                 115B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]phenyl]-(4- methylsulfonylphenyl)methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 617.2 
               
               
                   
               
               
                 116 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-(4- methylsulfonylphenyl)methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; chloride 
                 589.2 
               
               
                   
               
               
                 117 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[(7,8-difluoro-2,2-dimethyl- chroman-4- yl)carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 499.2 
               
               
                   
               
               
                 118 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 (26-ethyl-8,29-dioxo-17-oxa-1,9,27- triazapentacyclo[24.2.2.13,7.110,18. 011,16]dotriaconta- 3,5,7(32),11(16),12,14-hexaen-28- ylidene)ammonium; 2,2,2- trifluoroacetate 
                 503.3 
               
               
                   
               
               
                 119A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[(3- hydroxy-2,2-dimethyl-chroman-4- yl)carbamoyl]phenyl]butyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 521.3 
               
               
                   
               
               
                 119B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[(3- hydroxy-2,2-dimethyl-chroman-4- yl)carbamoyl]phenyl]butyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 521.3 
               
               
                   
               
               
                 120A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 (26-ethyl-8,29-dioxo-17-oxa-1,9,27- triazapentacyclo[24.2.2.13,7.010,19. 011,16]hentriaconta- 3,5,7(31),11(16),12,14-hexaen-28- ylidene)ammonium; 2,2,2- trifluoroacetate 
                 489.3 
               
               
                   
               
               
                 120B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 (26-ethyl-8,29-dioxo-17-oxa-1,9,27- triazapentacyclo[24.2.2.13,7.010,19. 011,16]hentriaconta- 3,5,7(31),11(16),12,14-hexaen-28- ylidene)ammonium; 2,2,2- trifluoroacetate 
                 489.3 
               
               
                   
               
               
                 120C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 (26-ethyl-8,29-dioxo-17-oxa-1,9,27- triazapentacyclo[24.2.2.13,7.010,19. 011,16]hentriaconta- 3,5,7(31),11(16),12,14-hexaen-28- ylidene)ammonium; 2,2,2- trifluoroacetate 
                 489.3 
               
               
                   
               
               
                 120D 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 (26-ethyl-8,29-dioxo-17-oxa-1,9,27- triazapentacyclo[24.2.2.13,7.010,19. 011,16]hentriaconta- 3,5,7(31),11(16),12,14-hexaen-28- ylidene)ammonium; 2,2,2- trifluoroacetate 
                 489.3 
               
               
                   
               
               
                 121 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)methyl]- N-(2-hydroxy-2-methyl-indan-1- yl)benzamide 
                 449.3 
               
               
                   
               
               
                 122A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)butyl]-N-(2-hydroxy-2-methyl- indan-1-yl)benzamide 
                 491.3 
               
               
                   
               
               
                 122B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)butyl]-N-(2-hydroxy-2-methyl- indan-1-yl)benzamide 
                 491.3 
               
               
                   
               
               
                 122C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)butyl]-N-(2-hydroxy-2-methyl- indan-1-yl)benzamide 
                 491.3 
               
               
                   
               
               
                 122D 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)butyl]-N-(2-hydroxy-2-methyl- indan-1-yl)benzamide 
                 491.3 
               
               
                   
               
               
                 123A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-(3- methylsulfonylphenyl)methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 589.3 
               
               
                   
               
               
                 123B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-(3- methylsulfonylphenyl)methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 589.3 
               
               
                   
               
               
                 124A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-(3- methylsulfonylphenyl)methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 589.2 
               
               
                   
               
               
                 124B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-(3- methylsulfonylphenyl)methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 589.2 
               
               
                   
               
               
                 125 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[5-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]pyridin-1-ium-3-yl]- 3-methoxy-propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 494.3 
               
               
                   
               
               
                 126A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)methyl]- N-(3-hydroxychroman-4- yl)benzamide 
                 451.3 
               
               
                   
               
               
                 126B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)methyl]- N-(3-hydroxychroman-4- yl)benzamide 
                 451.4 
               
               
                   
               
               
                 127A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)butyl]-N-(3-hydroxychroman-4- yl)benzamide 
                 493.4 
               
               
                   
               
               
                 127B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)butyl]-N-(3-hydroxychroman-4- yl)benzamide 
                 493.4 
               
               
                   
               
               
                 127C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)butyl]-N-(3-hydroxychroman-4- yl)benzamide 
                 493.3 
               
               
                   
               
               
                 127D 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)butyl]-N-(3-hydroxychroman-4- yl)benzamide 
                 493.3 
               
               
                   
               
               
                 128 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[(3-hydroxy- 2,2-dimethyl-chroman-4- yl)carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 537.3 
               
               
                   
               
               
                 129A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-fluoro-5-[(3- hydroxy-2,2-dimethyl-chroman-4- yl)carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 555.3 
               
               
                   
               
               
                 129B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-fluoro-5-[(3- hydroxy-2,2-dimethyl-chroman-4- yl)carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 555.2 
               
               
                   
               
               
                 130 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[1-[5-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]pyridin-1-ium-3-yl]- 3-methoxy-propyl]-4-isopropyl-4- methyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 494.3 
               
               
                   
               
               
                 131A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)methyl]- N-(3-hydroxy-3-methyl-chroman-4- yl)benzamide 
                 465.3 
               
               
                   
               
               
                 131B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)methyl]- N-(3-hydroxy-3-methyl-chroman-4- yl)benzamide 
                 465.1 
               
               
                   
               
               
                 132A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [[(2R,4S)-2- (trifluoromethyl)chroman-4- yl]carbamoyl]phenyl]propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 561.3 
               
               
                   
               
               
                 132B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[3-methoxy-1-[3- [[(2R,4S)-2- (trifluoromethyl)chroman-4- yl]carbamoyl]phenyl]propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 561.3 
               
               
                   
               
               
                 133 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[4-[(3-hydroxy-2,2- dimethyl-chroman-4- yl)carbamoyl]pyridin-1-ium-2- yl]methyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 480.3 
               
               
                   
               
               
                 134 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[[4-[[(2R,4S)- 2-(trifluoromethyl)chroman-4- yl]carbamoyl]pyridin-1-ium-2- yl]methyl]hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 504.3 
               
               
                   
               
               
                 135 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]phenyl]-3-morpholin- 4-ium-4-yl-propyl]-4,4-diethyl-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 576.4 
               
               
                   
               
               
                 136 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[3-morpholin-4-ium- 4-yl-1-[3-[[(2R,4S)-2- (trifluoromethyl)chroman-4- yl]carbamoyl]phenyl]propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 616.3 
               
               
                   
               
               
                 137 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[(6-fluoro-2,2- dimethyl-chroman-4- yl)carbamoyl]phenyl]-3-morpholin- 4-ium-4-yl-propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 594.4 
               
               
                   
               
               
                 138 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-fluoro-5- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 511.3 
               
               
                   
               
               
                 139 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1S)-1-[3-[[(3S,4R)- 3-hydroxy-2,2-dimethyl-chroman-4- yl]carbamoyl]phenyl]-2-(2- methoxyethoxy)ethyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 567.3 
               
               
                   
               
               
                 140 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1S)-1-[3-[[(1R,2R)- 2-hydroxyindan-1- yl]carbamoyl]phenyl]-2-(2- methoxyethoxy)ethyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 523.3 
               
               
                   
               
               
                 141 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-fluoro-5- [[(3S,4R)-3-hydroxy-2,2-dimethyl- chroman-4- yl]carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 497.3 
               
               
                   
               
               
                 142A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-(2-hydroxy-2- methyl-indan-1-yl)benzamide 
                 507.2 
               
               
                   
               
               
                 142B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-(2-hydroxy-2- methyl-indan-1-yl)benzamide 
                 507.2 
               
               
                   
               
               
                 143A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-(3-hydroxy-3- methyl-chroman-4-yl)benzamide 
                 523.3 
               
               
                   
               
               
                 143B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-(3-hydroxy-3- methyl-chroman-4-yl)benzamide 
                 523.3 
               
               
                   
               
               
                 144A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-5-fluoro-N-(2- hydroxy-2-methyl-indan-1- yl)benzamide 
                 525.3 
               
               
                   
               
               
                 144B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-5-fluoro-N-(2- hydroxy-2-methyl-indan-1- yl)benzamide 
                 525.3 
               
               
                   
               
               
                 144C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-5-fluoro-N-(2- hydroxy-2-methyl-indan-1- yl)benzamide 
                 525.3 
               
               
                   
               
               
                 144D 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-5-fluoro-N-(2- hydroxy-2-methyl-indan-1- yl)benzamide 
                 525.3 
               
               
                   
               
               
                 145 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- hydroxy-3-methyl-butyl]-N-[(4S)- 2,2-dimethylchroman-4- yl]benzamide 
                 535.3 
               
               
                   
               
               
                 146A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1S)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1-yl)-2-(2- methoxyethoxy)ethyl]-N-(2- hydroxy-2-methyl-indan-1- yl)benzamide 
                 537.3 
               
               
                   
               
               
                 146B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1S)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1-yl)-2-(2- methoxyethoxy)ethyl]-N-(2- hydroxy-2-methyl-indan-1- yl)benzamide 
                 537.3 
               
               
                   
               
               
                 147 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[(6-fluoro-3- hydroxy-2,2-dimethyl-chroman-4- yl)carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 497.3 
               
               
                   
               
               
                 148 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-fluoro-5-[(6- fluoro-3-hydroxy-2,2-dimethyl- chroman-4- yl)carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 515.3 
               
               
                   
               
               
                 149A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-(3- fluorosulfonyloxyphenyl)methyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 609.3 
               
               
                   
               
               
                 149B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-(3- fluorosulfonyloxyphenyl)methyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 609.2 
               
               
                   
               
               
                 150 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-(4- fluorosulfonyloxyphenyl)methyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 609.2 
               
               
                   
               
               
                 151 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[3- hydroxy-2- (trifluoromethyl)chroman-4- yl]carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 577.3 
               
               
                   
               
               
                 152A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[(3R,4R)- 3-hydroxychroman-4- yl]carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 509.2 
               
               
                   
               
               
                 152B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[(3S,4R)- 3-hydroxychroman-4- yl]carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 509.2 
               
               
                   
               
               
                 153 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-3- methylsulfonyl-propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 541.3 
               
               
                   
               
               
                 154A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methylsulfonyl-propyl]-N-[(1R,2R)- 2-hydroxy-2-methyl-indan-1- yl]benzamide 
                 555.3 
               
               
                   
               
               
                 154B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methylsulfonyl-propyl]-N-[(1R,2S)- 2-hydroxy-2-methyl-indan-1- yl]benzamide 
                 555.2 
               
               
                   
               
               
                 155 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[[(3S,4R)-3- hydroxy-2,2-dimethyl-chroman-4- yl]carbamoyl]phenyl]-3- methylsulfonyl-propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 585.3 
               
               
                   
               
               
                 156 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]phenyl]-3- methylsulfonyl-propyl]-4,4-diethyl- 6-oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 569.3 
               
               
                   
               
               
                 157 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[3-(difluoromethoxy)-1-[3- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]propyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 529.3 
               
               
                   
               
               
                 158A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- (difluoromethoxy)propyl]-N- [(1R,2R)-2-hydroxy-2-methyl- indan-1-yl]benzamide 
                 543.4 
               
               
                   
               
               
                 158B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- (difluoromethoxy)propyl]-N- [(1R,2S)-2-hydroxy-2-methyl- indan-1-yl]benzamide 
                 543.3 
               
               
                   
               
               
                 159 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[3-(difluoromethoxy)-1-[3- [[(3S,4R)-3-hydroxy-2,2-dimethyl- chroman-4- yl]carbamoyl]phenyl]propyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 573.3 
               
               
                   
               
               
                 160 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[3-(difluoromethoxy)-1-[3- [[(4S)-2,2-dimethylchroman-4- yl]carbamoyl]phenyl]propyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 557.3 
               
               
                   
               
               
                 161 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-3- methylsulfonyl-propyl]-4,4-diethyl- 6-oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 541.3 
               
               
                   
               
               
                 162 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[3-methylsulfonyl-1- [3-[[(2R,4S)-2- (trifluoromethyl)chroman-4- yl]carbamoyl]phenyl]propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 609.3 
               
               
                   
               
               
                 163A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)butyl]-N-[(3S,4R)-3-hydroxy-3- methyl-chroman-4-yl]benzamide 
                 507.2 
               
               
                   
               
               
                 163B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)butyl]-N-[(3R,4R)-3-hydroxy-3- methyl-chroman-4-yl]benzamide 
                 507.4 
               
               
                   
               
               
                 164 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]-3- (difluoromethoxy)propyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 529.2 
               
               
                   
               
               
                 165A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[(3R,4S)- 6-fluoro-3-hydroxy-2,2-dimethyl- chroman-4-yl]carbamoyl]phenyl]-3- methoxy-propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 555.3 
               
               
                   
               
               
                 165B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[(3S,4R)- 6-fluoro-3-hydroxy-2,2-dimethyl- chroman-4-yl]carbamoyl]phenyl]-3- methoxy-propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 555.4 
               
               
                   
               
               
                 166A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-[(1R,2R)-2-hydroxy-2-methyl- indan-1-yl]-3-[(1R)-1-(2-imino-4,4- dimethyl-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]benzamide 
                 479.3 
               
               
                   
               
               
                 166B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-[(1R,2S)-2-hydroxy-2-methyl- indan-1-yl]-3-[(1R)-1-(2-imino-4,4- dimethyl-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]benzamide 
                 479.3 
               
               
                   
               
               
                 167 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-1-[3-[(2′- hydroxyspiro[cyclopropane-1,3′- indane]-1′-yl)carbamoyl]phenyl]-3- methoxy-propyl]-4,4-dimethyl-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 491.3 
               
               
                   
               
               
                 168 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4-ethyl-1-[(1R)-1-[3-[(2′- hydroxyspiro[cyclopropane-1,3′- indane]-1′-yl)carbamoyl]phenyl]-3- methoxy-propyl]-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 505.2 
               
               
                   
               
               
                 169 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-fluoro-5-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-3-methoxy- propyl]-4,4-dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 483.3 
               
               
                   
               
               
                 170 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[(6-fluoro- 2-hydroxy-indan-1- yl)carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 511.3 
               
               
                   
               
               
                 171 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[3-ethoxy-1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]propyl]-4,4- dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 479.3 
               
               
                   
               
               
                 172 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [(4S)-1-[3-(difluoromethoxy)-1-[3- [[(1R,2R)-2-hydroxyindan-1- yl]carbamoyl]phenyl]propyl]-4- ethyl-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 515.3 
               
               
                   
               
               
                 173 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-2,2- dimethylchroman-4-yl]carbamoyl]- 5-fluoro-phenyl]-3-methoxy- propyl]-4,4-dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 511.3 
               
               
                   
               
               
                 174 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-fluoro-5-[[(3S,4R)-3- hydroxy-2,2-dimethyl-chroman-4- yl]carbamoyl]phenyl]-3-methoxy- propyl]-4,4-dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 527.3 
               
               
                   
               
               
                 175A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-fluoro-N-[(1R,2R)-2-hydroxy-2- methyl-indan-1-yl]-5-[1-(2-imino- 4,4-dimethyl-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]benzamide 
                 497.3 
               
               
                   
               
               
                 175B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-fluoro-N-[(1R,2S)-2-hydroxy-2- methyl-indan-1-yl]-5-[1-(2-imino- 4,4-dimethyl-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]benzamide 
                 497.3 
               
               
                   
               
               
                 176A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-fluoro-5- [[2-(methoxymethyl)-2-methyl-3H- benzofuran-3- yl]carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 555.3 
               
               
                   
               
               
                 176B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-fluoro-5- [[2-(methoxymethyl)-2-methyl-3H- benzofuran-3- yl]carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 555.3 
               
               
                   
               
               
                 176C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-fluoro-5- [[2-(methoxymethyl)-2-methyl-3H- benzofuran-3- yl]carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 555.3 
               
               
                   
               
               
                 177 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-1-[3-[[(4S)-2,2- dimethylchroman-4- yl]carbamoyl]phenyl]-3-methoxy- propyl]-4,4-dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 493.3 
               
               
                   
               
               
                 178 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-1-[3-[[(3S,4R)-3-hydroxy- 2,2-dimethyl-chroman-4- yl]carbamoyl]phenyl]-3-methoxy- propyl]-4,4-dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 509.3 
               
               
                   
               
               
                 179 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-3-methoxy- propyl]-4,4-dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 465.3 
               
               
                   
               
               
                 180A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[2- (hydroxymethyl)-2-methyl-3H- benzofuran-3- yl]carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 523.3 
               
               
                   
               
               
                 180B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[2- (hydroxymethyl)-2-methyl-3H- benzofuran-3- yl]carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 523.3 
               
               
                   
               
               
                 181A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-1-[3-[(2,2-dimethyl-3H- benzofuran-3- yl)carbamoyl]phenyl]-3-methoxy- propyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 507.3 
               
               
                   
               
               
                 181B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-1-[3-[(2,2-dimethyl-3H- benzofuran-3- yl)carbamoyl]phenyl]-3-methoxy- propyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 507.3 
               
               
                   
               
               
                 182 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(3S,4R)-6-fluoro-3- hydroxy-2,2-dimethyl-chroman-4- yl]carbamoyl]phenyl]methyl]-4,4- dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 469.3 
               
               
                   
               
               
                 183 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-fluoro-5-[[(3S,4R)-3- hydroxy-2,2-dimethyl-chroman-4- yl]carbamoyl]phenyl]methyl]-4,4- dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 469.2 
               
               
                   
               
               
                 184 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)methyl]- N-(3-hydroxy-2,2,3-trimethyl- chroman-4-yl)benzamide 
                 493.3 
               
               
                   
               
               
                 185 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-(3-hydroxy-2,2,3-trimethyl- chroman-4-yl)-3-[(2-imino-4,4- dimethyl-6-oxo- hexahydropyrimidin-1- yl)methyl]benzamide 
                 465.3 
               
               
                   
               
               
                 186 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[(2,2-dimethyl-3H- benzofuran-3- yl)carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 449.3 
               
               
                   
               
               
                 187A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[[2- (hydroxymethyl)-2-methyl-3H- benzofuran-3- yl]carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 465.3 
               
               
                   
               
               
                 187B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[[2- (hydroxymethyl)-2-methyl-3H- benzofuran-3- yl]carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 465.3 
               
               
                   
               
               
                 188 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-1-[3-[[2-hydroxy-2- (trifluoromethyl)indan-1- yl]carbamoyl]phenyl]-3-methoxy- propyl]-4,4-dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 533.2 
               
               
                   
               
               
                 189A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-1-[3-[(3,3-difluoro-2- hydroxy-indan-1- yl)carbamoyl]phenyl]-3-methoxy- propyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 529.1 
               
               
                   
               
               
                 189B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-1-[3-[(3,3-difluoro-2- hydroxy-indan-1- yl)carbamoyl]phenyl]-3-methoxy- propyl]-4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; chloride 
                 529.1 
               
               
                   
               
               
                 190 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[(3,3-difluoro-2-hydroxy- indan-1- yl)carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 471.2 
               
               
                   
               
               
                 191A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[(2′- hydroxyspiro[cyclopropane-1,3′- indane]-1′-yl)carbamoyl]phenyl]-3- methoxy-propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 519.2 
               
               
                   
               
               
                 191B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[(2′- hydroxyspiro[cyclopropane-1,3′- indane]-1′-yl)carbamoyl]phenyl]-3- methoxy-propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 519.2 
               
               
                   
               
               
                 192A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)ethyl]-N-[(1R,2R)-2-hydroxy-2- methyl-indan-1-yl]benzamide 
                 463.3 
               
               
                   
               
               
                 192B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)ethyl]-N-[(1R,2S)-2-hydroxy-2- methyl-indan-1-yl]benzamide 
                 463.3 
               
               
                   
               
               
                 193 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[(3S,4R)- 3-hydroxy-2,2-dimethyl-chroman-4- yl]carbamoyl]phenyl]ethyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 493.3 
               
               
                   
               
               
                 194 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(3S,4R)-3-hydroxy-2,2- dimethyl-chroman-4- yl]carbamoyl]phenyl]methyl]-4,4- dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 451.3 
               
               
                   
               
               
                 195A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4-ethyl-1-[(1R)-1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-3-methoxy- propyl]-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 479.3 
               
               
                   
               
               
                 195B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4-ethyl-1-[(1R)-1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]-3-methoxy- propyl]-4-methyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 479.2 
               
               
                   
               
               
                 196A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4-ethyl-2-imino-4- methyl-6-oxo-hexahydropyrimidin- 1-yl)-3-methoxy-propyl]-N- [(1R,2S)-2-hydroxy-2-methyl- indan-1-yl]benzamide 
                 493.3 
               
               
                   
               
               
                 196B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4-ethyl-2-imino-4- methyl-6-oxo-hexahydropyrimidin- 1-yl)-3-methoxy-propyl]-N- [(1R,2S)-2-hydroxy-2-methyl- indan-1-yl]benzamide 
                 493.2 
               
               
                   
               
               
                 196C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4-ethyl-2-imino-4- methyl-6-oxo-hexahydropyrimidin- 1-yl)-3-methoxy-propyl]-N- [(1R,2R)-2-hydroxy-2-methyl- indan-1-yl]benzamide 
                 493.3 
               
               
                   
               
               
                 196D 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4-ethyl-2-imino-4- methyl-6-oxo-hexahydropyrimidin- l-yl)-3-methoxy-propyl]-N- [(1R,2R)-2-hydroxy-2-methyl- indan-1-yl]benzamide 
                 493.2 
               
               
                   
               
               
                 197 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-fluoro-5-[(6-fluoro-3- hydroxy-2,2-dimethyl-chroman-4- yl)carbamoyl]phenyl]methyl]-4,4- dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 487.3 
               
               
                   
               
               
                 198 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[[(4S)-2,2-dimethylchroman- 4-yl]carbamoyl]phenyl]methyl]-4,4- dimethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 435.3 
               
               
                   
               
               
                 199 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[[3-[(6-fluoro-2- hydroxy-indan-1- yl)carbamoyl]phenyl]methyl]-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 453.2 
               
               
                   
               
               
                 200 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-1-[3-[(6-fluoro-2-hydroxy- indan-1-yl)carbamoyl]phenyl]-3- methoxy-propyl]-4,4-dimethyl-6- oxo-hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 483.3 
               
               
                   
               
               
                 201 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[(1R)-3-[2-[2-(2- azidoethoxy)ethoxy]ethoxy]-1-[3- [[(4S)-2,2-dimethylchroman-4- yl]carbamoyl]phenyl]propyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 664.4 
               
               
                   
               
               
                 202 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[(1R,2S)- 2-hydroxyindan-1- yl]carbamoyl]phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 493.3 
               
               
                   
               
               
                 203 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-(3-hydroxy- 2,2,3-trimethyl-chroman-4- yl)benzamide 
                 551.4 
               
               
                   
               
               
                 204A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[2-hydroxy-2- (trifluoromethyl)indan-1- yl]benzamide 
                 561.3 
               
               
                   
               
               
                 204B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[2-hydroxy-2- (trifluoromethyl)indan-1- yl]benzamide 
                 561.3 
               
               
                   
               
               
                 205A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[(3S,4R)- 3-hydroxy-3-methyl-chroman-4- yl]carbamoyl]phenyl]ethyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 479.3 
               
               
                   
               
               
                 205B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[(1R)-1-[3-[[(3R,4R)- 3-hydroxy-3-methyl-chroman-4- yl]carbamoyl]phenyl]ethyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 479.3 
               
               
                   
               
               
                 206A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)propyl]- N-[(3S,4R)-3-hydroxy-3-methyl- chroman-4-yl]benzamide 
                 493.3 
               
               
                   
               
               
                 206B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[[(3R,4R)-3- hydroxy-3-methyl-chroman-4- yl]carbamoyl]phenyl]propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 493.3 
               
               
                   
               
               
                 207A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[[(3S,4R)-3- hydroxy-2,2-dimethyl-chroman-4- yl]carbamoyl]phenyl]propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 507.3 
               
               
                   
               
               
                 207B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[[(3S,4R)-3- hydroxy-2,2-dimethyl-chroman-4- yl]carbamoyl]phenyl]propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 507.3 
               
               
                   
               
               
                 208 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-[[(1R,2R)-2- hydroxyindan-1- yl]carbamoyl]phenyl]propyl]-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 463.3 
               
               
                   
               
               
                 209 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-LL(4R)-chroman-4- yl]carbamoyl]phenyl]pent-4-ynyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 487.6 
               
               
                   
               
               
                 210A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]hex-5-ynyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 501.6 
               
               
                   
               
               
                 210B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4R)-chroman-4- yl]carbamoyl]phenyl]hex-5-ynyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 501.6 
               
               
                   
               
               
                 210C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[1-[3-[[(4S)-chroman-4- yl]carbamoyl]phenyl]hex-5-ynyl]- 4,4-diethyl-6-oxo- hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 501.6 
               
               
                   
               
               
                 211A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[(1R)-1-[3- [[(4S)-spiro[chromane-2,1′- cyclobutane]-4- yl]carbamoyl]phenyl]pent-4- ynyl]hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 527.4 
               
               
                   
               
               
                 211B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[(1R)-1-[3- [[(4R)-spiro[chromane-2,1′- cyclobutane]-4- yl]carbamoyl]phenyl]pent-4- ynyl]hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 527.4 
               
               
                   
               
               
                 212A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[(1R)-1-[3- [[(4S)-spiro[chromane-2,1′- cyclobutane]-4- yl]carbamoyl]phenyl]butyl]hexahyd ropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 517.4 
               
               
                   
               
               
                 212B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[(1R)-1-[3- [[(4R)-spiro[chromane-2,1′- cyclobutane]-4- yl]carbamoyl]phenyl]butyl]hexahyd ropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 517.4 
               
               
                   
               
               
                 213 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[(S)-1-[3- [[(4R)-spiro[chromane-2,1′- cyclobutane]-4- yl]carbamoyl]phenyl]pent-4- ynyl]hexahydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 527.4 
               
               
                   
               
               
                 214A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1S)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1-yl)pent- 4-ynyl]-N-(2,2-dimethylchroman-4- yl)benzamide 
                 515.5 
               
               
                   
               
               
                 214B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1-yl)pent- 4-ynyl]-N-(2,2-dimethylchroman-4- yl)benzamide 
                 515.6 
               
               
                   
               
               
                 214C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1-yl)pent- 4-ynyl]-N-(2,2-dimethylchroman-4- yl)benzamide 
                 515.5 
               
               
                   
               
               
                 215A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-(6-chlorochroman-4-yl)-3-[(4,4- diethyl-2-imino-6-oxo- hexahydropyrimidin-1- yl)methyl]benzamide 
                 469.5 
               
               
                   
               
               
                 215B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-(6-chlorochroman-4-yl)-3-[(4,4- diethyl-2-imino-6-oxo- hexahydropyrimidin-1- yl)methyl]benzamide 
                 469.5 
               
               
                   
               
               
                 216 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)methyl]- N-(3,3-dimethylchroman-4- yl)benzamide 
                 463.6 
               
               
                   
               
               
                 217 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)methyl]- N-(6-methylchroman-4- yl)benzamide 
                 449.6 
               
               
                   
               
               
                 218A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[[3-[(2- phenylchroman-4- yl)carbamoyl]phenyl]methyl]hexa- hydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 511.7 
               
               
                   
               
               
                 218B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-6-oxo-1-[[3-[(2- phenylchroman-4- yl)carbamoyl]phenyl]methyl]hexa- hydropyrimidin-2- ylidene]ammonium; 2,2,2- trifluoroacetate 
                 511.6 
               
               
                   
               
               
                 219 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [1-[[3-[(6-bromochroman-4- yl)carbamoyl]phenyl]methyl]-4,4- diethyl-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                  513.5,  515.5 
               
               
                   
               
               
                 220 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 [4,4-diethyl-1-[1-[3-fluoro-5- (2,3,4,5-tetrahydro-1-benzoxepin-5- ylcarbamoyl)phenyl]-3-methoxy- propyl]-6-oxo-hexahydropyrimidin- 2-ylidene]ammonium; 2,2,2- trifluoroacetate 
                 525.4 
               
               
                   
               
               
                 221 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1S)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1-yl)pent- 4-ynyl]-N-[(1R,2R)-2- hydroxyindan-1-yl]benzamide 
                 487.5 
               
               
                   
               
            
           
         
       
     
     The following Examples were made according to the Scheme described above using appropriate starting materials 
     
       
         
           
               
               
               
               
             
               
                   
               
               
                 Ex. 
                   
                   
                 LC/MS 
               
               
                 No. 
                 Structure 
                 Name 
                 (M + 1)+ 
               
               
                   
               
             
            
               
                 222 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-(3-hydroxy-2,2,3-trimethyl- chroman-4-yl)-3-[(1R)-1-(2- imino-4,4-dimethyl-6-oxo- hexahydropyrimidin-1- yl)ethyl]benzamide 
                 479.2 
               
               
                   
               
               
                 223 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-2- methoxy-ethyl]-N-[(1R,2R)-2- hydroxyindan-1-yl]benzamide 
                 479.2 
               
               
                   
               
               
                 224 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-[(1R,2R)-2-hydroxyindan-1- yl]-3-[3,3,3-trifluoro-1-(2-imino- 4,4-dimethyl-6-oxo- hexahydropyrimidin-1- yl)propyl]benzamide 
                 489.3 
               
               
                   
               
               
                 225 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-[(4R)-4-ethyl-2-imino- 4-methyl-6-oxo- hexahydropyrimidin-1-yl]ethyl]- N-(3-hydroxy-2,2,3-trimethyl- chroman-4-yl)benzamide 
                 493.2 
               
               
                   
               
               
                 226 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-(2- hydroxytetralin-1-yl)benzamide 
                 507.4 
               
               
                   
               
               
                 227A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-5- methyl-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[(1R,2R)-2- hydroxyindan-1-yl]benzamide 
                 507.3 
               
               
                   
               
               
                 227B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-5- methyl-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[(1R,2R)-2- hydroxyindan-1-yl]benzamide 
                 507.3 
               
               
                   
               
               
                 228 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-(4,4-diethyl-2-imino-6- oxo-hexahydropyrimidin-1- yl)ethyl]-N-[(3S,4R)-3-hydroxy- 2,2,3-trimethyl-chroman-4- yl]benzamide 
                 507.1 
               
               
                   
               
               
                 229 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-2- methoxy-ethyl]-N-[(3R,4R)-3- hydroxy-3-methyl-chroman-4- yl]benzamide 
                 509.2 
               
               
                   
               
               
                 230 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[l-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-2- methoxy-ethyl]-N-[(3S,4R)-3- hydroxy-3-methyl-chroman-4- yl]benzamide 
                 509.2 
               
               
                   
               
               
                 231 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-[(3S,4R)-3-hydroxy-2,2,3- trimethyl-chroman-4-yl]-3-[(1R)- 1-(2-imino-4,4-dimethyl-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]benzamide 
                 523 
               
               
                   
               
               
                 232 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[l-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-2- methoxy-ethyl]-N-[(3S,4R)-3- hydroxy-2,2-dimethyl-chroman-4- yl]benzamide 
                 523.3 
               
               
                   
               
               
                 233 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-[(3S,4R)-3-hydroxy-2,2- dimethyl-chroman-4-yl]-3-[3,3,3- trifluoro-1-(2-imino-4,4-dimethyl- 6-oxo-hexahydropyrimidin-1- yl)propyl]benzamide 
                 533.3 
               
               
                   
               
               
                 234A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-[(4R)-4-ethyl-2-imino- 4-methyl-6-oxo- hexahydropyrimidin-1-yl]-3- methoxy-propyl]-N-(3-hydroxy- 2,2,3-trimethyl-chroman-4- yl)benzamide 
                 537.3 
               
               
                   
               
               
                 234B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-[(4R)-4-ethyl-2-imino- 4-methyl-6-oxo- hexahydropyrimidin-1-yl]-3- methoxy-propyl]-N-(3-hydroxy- 2,2,3-trimethyl-chroman-4- yl)benzamide 
                 537.3 
               
               
                   
               
               
                 235 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-(6-fluoro-3-hydroxy-2,2,3- trimethyl-chroman-4-yl)-3-[(1R)- 1-(2-imino-4,4-dimethyl-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]benzamide 
                 541.3 
               
               
                   
               
               
                 236A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-4,4,4- trifluoro-3-hydroxy-butyl]-N- [(1R,2R)-2-hydroxyindan-1- yl]benzamide 
                 547.3 
               
               
                   
               
               
                 236B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[l-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-4,4,4- trifluoro-3-hydroxy-butyl]-N- [(1R,2R)-2-hydroxyindan-1- yl]benzamide 
                 547.3 
               
               
                   
               
               
                 236C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[l-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-4,4,4- trifluoro-3-hydroxy-butyl]-N- [(1R,2R)-2-hydroxyindan-1- yl]benzamide 
                 547.3 
               
               
                   
               
               
                 237A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-[(4R)-4-ethyl-2-imino- 4-methyl-6-oxo- hexahydropyrimidin-1-yl]-3- methoxy-propyl]-N-(6-fluoro-3- hydroxy-2,2,3-trimethyl-chroman- 4-yl)benzamide 
                 555.4 
               
               
                   
               
               
                 237B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[(1R)-1-[(4R)-4-ethyl-2-imino- 4-methyl-6-oxo- hexahydropyrimidin-1-yl]-3- methoxy-propyl]-N-(6-fluoro-3- hydroxy-2,2,3-trimethyl-chroman- 4-yl)benzamide 
                 555.4 
               
               
                   
               
               
                 238A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-4,4,4- trifluoro-3-methoxy-butyl]-N- [(1R,2R)-2-hydroxyindan-1- yl]benzamide 
                 561.3 
               
               
                   
               
               
                 238B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-4,4,4- trifluoro-3-methoxy-butyl]-N- [(1R,2R)-2-hydroxyindan-1- yl]benzamide 
                 561.3 
               
               
                   
               
               
                 238C 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-4,4,4- trifluoro-3-methoxy-butyl]-N- [(1R,2R)-2-hydroxyindan-1- yl]benzamide 
                 561.3 
               
               
                   
               
               
                 238D 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-4,4,4- trifluoro-3-methoxy-butyl]-N- [(1R,2R)-2-hydroxyindan-1- yl]benzamide 
                 561.3 
               
               
                   
               
               
                 239 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[(1R,2R)-2- hydroxyindan-1-yl]-2- (trifluoromethyl)benzamide 
                 561.3 
               
               
                   
               
               
                 240A 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[3-(difluoromethoxy)-1-[(4R)- 4-ethyl-2-imino-4-methyl-6-oxo- hexahydropyrimidin-1-yl]propyl]- N-(3-hydroxy-2,2,3-trimethyl- chroman-4-yl)benzamide 
                 573.3 
               
               
                   
               
               
                 240B 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-[3-(difluoromethoxy)-1-[(4R)- 4-ethyl-2-imino-4-methyl-6-oxo- hexahydropyrimidin-1-yl]propyl]- N-(3-hydroxy-2,2,3-trimethyl- chroman-4-yl)benzamide 
                 573.3 
               
               
                   
               
               
                 241 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[(1R,2R)-2- hydroxy-2-methyl-indan-1-yl]-2- (trifluoromethyl)benzamide 
                 575.3 
               
               
                   
               
               
                 242 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[(1R,2S)-2- hydroxy-2-methyl-indan-1-yl]-2- (trifluoromethyl)benzamide 
                 575.4 
               
               
                   
               
               
                 243 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[(3S,4R)-3- hydroxychroman-4-yl]-2- (trifluoromethyl)benzamide 
                 577.3 
               
               
                   
               
               
                 244 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[(3S,4R)-3- hydroxy-3-methyl-chroman-4-yl]- 2-(trifluoromethyl)benzamide 
                 591.3 
               
               
                   
               
               
                 245 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[(3R,4R)-3- hydroxy-3-methyl-chroman-4-yl]- 2-(trifluoromethyl)benzamide 
                 591.3 
               
               
                   
               
               
                 246 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5-[1-(4,4-diethyl-2-imino-6-oxo- hexahydropyrimidin-1-yl)-3- methoxy-propyl]-N-[(3S,4R)-3- hydroxy-2,2-dimethyl-chroman-4- yl]-2-(trifluoromethyl)benzamide 
                 605.4 
               
               
                   
               
            
           
         
       
     
     Assay 
     The parasite stock was maintained at 4% haematocrit in RPMI-Hepes media buffered with sodium bicarbonate and supplemented with 5% heat inactivated human serum and 0.5% albumax. 
     Approximately 42 hours prior to the potency assay being set up, parasites were synchronized with 5% sorbitol to select for ring stage parasites. On the day of assay set up, a blood smear of the parasite culture was Giemsa stained and counted. The parasitemia was adjusted to 0.7% rings and the haematocrit was diluted to 2% in RPMI-Hepes media buffered with sodium bicarbonate and supplemented with 5% heat inactivated human serum and 0.5% albumax. 30 ul of diluted parasites are then added into 10 ul of media+compound in pre-prepared Greiner TC assay plates. Parasite assay plates were placed in gassed humidified boxes in single layer and allowed to incubate at 37° C. for 72 hours. After 72 hours growth, assay plates are sealed with parafilm and frozen flat, in single file at −80° C. overnight. On the following day, assay plates are allowed to thaw at room temperature for 4 hours to which an LDH assay is performed to measure parasite growth. 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 Example No. 
                 EC50 (nM) 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                  1 
                 0.8 
               
               
                   
                  2 
                 1.3 
               
               
                   
                  3 
                 6.2 
               
               
                   
                  4A 
                 0.4 
               
               
                   
                  4B 
                 35.4 
               
               
                   
                  5A 
                 16.2 
               
               
                   
                  5B 
                 57.4 
               
               
                   
                  6A 
                 58.8 
               
               
                   
                  6B 
                 83.9 
               
               
                   
                  7 
                 39.2 
               
               
                   
                  8 
                 4.2 
               
               
                   
                  9A 
                 0.5 
               
               
                   
                  9B 
                 19.2 
               
               
                   
                  10 
                 7.9 
               
               
                   
                  11 
                 1.5 
               
               
                   
                  12 
                 1.4 
               
               
                   
                  13A 
                 0.3 
               
               
                   
                  13B 
                 43.8 
               
               
                   
                  14A 
                 0.2 
               
               
                   
                  14B 
                 55 
               
               
                   
                  15 
                 2.6 
               
               
                   
                  16 
                 0.3 
               
               
                   
                  17 
                 3.7 
               
               
                   
                  18 
                 21.5 
               
               
                   
                  19 
                 0.6 
               
               
                   
                  20 
                 10.9 
               
               
                   
                  21 
                 3.5 
               
               
                   
                  22 
                 17.2 
               
               
                   
                  23 
                 11.5 
               
               
                   
                  24 
                 10.1 
               
               
                   
                  25 
                 4.4 
               
               
                   
                  26 
                 8.9 
               
               
                   
                  27 
                 3.2 
               
               
                   
                  28 
                 1.1 
               
               
                   
                  29 
                 7.7 
               
               
                   
                  30 
                 3.1 
               
               
                   
                  31 
                 0.8 
               
               
                   
                  32 
                 2 
               
               
                   
                  33 
                 2.8 
               
               
                   
                  34 
                 0.5 
               
               
                   
                  35 
                 2 
               
               
                   
                  36 
                 16.8 
               
               
                   
                  37 
                 14.4 
               
               
                   
                  38 
                 31.3 
               
               
                   
                  39 
                 30.4 
               
               
                   
                  40 
                 9.7 
               
               
                   
                  41 
                 0.9 
               
               
                   
                  42 
                 10.3 
               
               
                   
                  43 
                 7.2 
               
               
                   
                  44 
                 2.6 
               
               
                   
                  45 
                 2.6 
               
               
                   
                  46 
                 10.4 
               
               
                   
                  47 
                 6.5 
               
               
                   
                  48 
                 5.2 
               
               
                   
                  49A 
                 0.7 
               
               
                   
                  49B 
                 85 
               
               
                   
                  50 
                 0.4 
               
               
                   
                  51A 
                 91.9 
               
               
                   
                  51B 
                 0.5 
               
               
                   
                  52 
                 20.1 
               
               
                   
                  53 
                 25.3 
               
               
                   
                  54 
                 5.6 
               
               
                   
                  55 
                 9.8 
               
               
                   
                  56 
                 0.5 
               
               
                   
                  57 
                 0.6 
               
               
                   
                  58 
                 1.3 
               
               
                   
                  59 
                 4.7 
               
               
                   
                  60 
                 5.8 
               
               
                   
                  61 
                 6.1 
               
               
                   
                  62A 
                 0.4 
               
               
                   
                  62B 
                 7.5 
               
               
                   
                  63 
                 80.2 
               
               
                   
                  64 
                 75.4 
               
               
                   
                  65 
                 43.9 
               
               
                   
                  66 
                 7.5 
               
               
                   
                  67 
                 2.7 
               
               
                   
                  68 
                 72.2 
               
               
                   
                  69 
                 29.3 
               
               
                   
                  70 
                 76 
               
               
                   
                  71 
                 9.3 
               
               
                   
                  72A 
                 25.5 
               
               
                   
                  72B 
                 0.3 
               
               
                   
                  73A 
                 0.7 
               
               
                   
                  74 
                 0.9 
               
               
                   
                  75 
                 66.5 
               
               
                   
                  76 
                 0.9 
               
               
                   
                  77 
                 14.7 
               
               
                   
                  78 
                 3.1 
               
               
                   
                  79 
                 2.1 
               
               
                   
                  80 
                 1.7 
               
               
                   
                  81 
                 1.3 
               
               
                   
                  82 
                 39.4 
               
               
                   
                  83A 
                 62.9 
               
               
                   
                  83B 
                 0.4 
               
               
                   
                  83C 
                 50.2 
               
               
                   
                  84 
                 8.3 
               
               
                   
                  85A 
                 1 
               
               
                   
                  85B 
                 2.1 
               
               
                   
                  86 
                 49.6 
               
               
                   
                  87 
                 7.3 
               
               
                   
                  88 
                 0.4 
               
               
                   
                  89A 
                 9.8 
               
               
                   
                  89B 
                 1.6 
               
               
                   
                  90A 
                 3.2 
               
               
                   
                  90B 
                 0.5 
               
               
                   
                  90C 
                 12 
               
               
                   
                  91A 
                 1.9 
               
               
                   
                  91B 
                 63.7 
               
               
                   
                  92A 
                 25.5 
               
               
                   
                  92B 
                 0.9 
               
               
                   
                  92C 
                 2.8 
               
               
                   
                  93 
                 1.8 
               
               
                   
                  94A 
                 0.4 
               
               
                   
                  94B 
                 72.5 
               
               
                   
                  95 
                 43.2 
               
               
                   
                  96 
                 19.9 
               
               
                   
                  97 
                 10.6 
               
               
                   
                  98A 
                 15.5 
               
               
                   
                  98B 
                 8.1 
               
               
                   
                  99A 
                 0.6 
               
               
                   
                  99B 
                 2 
               
               
                   
                 100A 
                 0.4 
               
               
                   
                 100B 
                 4.8 
               
               
                   
                 101A 
                 0.3 
               
               
                   
                 101B 
                 1.5 
               
               
                   
                 102 
                 0.5 
               
               
                   
                 103 
                 0.5 
               
               
                   
                 104 
                 0.9 
               
               
                   
                 105A 
                 0.5 
               
               
                   
                 105B 
                 0.6 
               
               
                   
                 106A 
                 0.5 
               
               
                   
                 106B 
                 3.1 
               
               
                   
                 107 
                 0.2 
               
               
                   
                 108A 
                 0.4 
               
               
                   
                 108B 
                 23.6 
               
               
                   
                 109A 
                 1.6 
               
               
                   
                 109B 
                 22.9 
               
               
                   
                 110A 
                 4 
               
               
                   
                 110B 
                 8.3 
               
               
                   
                 111 
                 0.4 
               
               
                   
                 112 A 
                 0.3 
               
               
                   
                 112B 
                 59.6 
               
               
                   
                 113 
                 96.1 
               
               
                   
                 114 A 
                 0.3 
               
               
                   
                 114B 
                 18.4 
               
               
                   
                 115A 
                 1 
               
               
                   
                 115B 
                 4.4 
               
               
                   
                 116 
                 6.8 
               
               
                   
                 117 
                 1.5 
               
               
                   
                 118 
                 56.5 
               
               
                   
                 119 A 
                 0.5 
               
               
                   
                 119B 
                 34 
               
               
                   
                 120A 
                 70.3 
               
               
                   
                 120B 
                 35.5 
               
               
                   
                 120C 
                 21.9 
               
               
                   
                 120D 
                 27.6 
               
               
                   
                 121 
                 8.6 
               
               
                   
                 122A 
                 1.2 
               
               
                   
                 122B 
                 17.7 
               
               
                   
                 122C 
                 57.3 
               
               
                   
                 122D 
                 0.6 
               
               
                   
                 123A 
                 1.9 
               
               
                   
                 123B 
                 9.3 
               
               
                   
                 124A 
                 9.9 
               
               
                   
                 124B 
                 53 
               
               
                   
                 125 
                 2.7 
               
               
                   
                 126A 
                 19.4 
               
               
                   
                 126B 
                 19.4 
               
               
                   
                 127A 
                 97.4 
               
               
                   
                 127B 
                 1.2 
               
               
                   
                 127C 
                 53.8 
               
               
                   
                 127D 
                 1.2 
               
               
                   
                 128 
                 1.1 
               
               
                   
                 129A 
                 0.8 
               
               
                   
                 129B 
                 87 
               
               
                   
                 130 
                 18.8 
               
               
                   
                 131A 
                 6.6 
               
               
                   
                 131B 
                 20.2 
               
               
                   
                 132A 
                 59 
               
               
                   
                 132B 
                 0.9 
               
               
                   
                 133 
                 3.3 
               
               
                   
                 134 
                 8.6 
               
               
                   
                 135 
                 1 
               
               
                   
                 136 
                 1.3 
               
               
                   
                 137 
                 0.8 
               
               
                   
                 138 
                 2.7 
               
               
                   
                 139 
                 1.5 
               
               
                   
                 140 
                 3.2 
               
               
                   
                 141 
                 0.8 
               
               
                   
                 142A 
                 2.1 
               
               
                   
                 142B 
                 1.4 
               
               
                   
                 143A 
                 2.2 
               
               
                   
                 143B 
                 1.8 
               
               
                   
                 144A 
                 81.6 
               
               
                   
                 144B 
                 1.9 
               
               
                   
                 144C 
                 99.5 
               
               
                   
                 144D 
                 0.9 
               
               
                   
                 145 
                 0.7 
               
               
                   
                 146A 
                 5.3 
               
               
                   
                 146B 
                 6.4 
               
               
                   
                 147 
                 1 
               
               
                   
                 148 
                 1 
               
               
                   
                 149A 
                 1.5 
               
               
                   
                 149B 
                 57.6 
               
               
                   
                 150 
                 2.8 
               
               
                   
                 151 
                 1.1 
               
               
                   
                 152A 
                 8.8 
               
               
                   
                 152B 
                 2 
               
               
                   
                 153 
                 77.8 
               
               
                   
                 154A 
                 54 
               
               
                   
                 154B 
                 69 
               
               
                   
                 155 
                 17.2 
               
               
                   
                 156 
                 5.7 
               
               
                   
                 157 
                 1.8 
               
               
                   
                 158A 
                 2.6 
               
               
                   
                 158B 
                 2.4 
               
               
                   
                 159 
                 0.8 
               
               
                   
                 160 
                 0.5 
               
               
                   
                 161 
                 23 
               
               
                   
                 162 
                 5.2 
               
               
                   
                 163A 
                 0.3 
               
               
                   
                 163B 
                 0.4 
               
               
                   
                 164 
                 0.9 
               
               
                   
                 165A 
                 41.4 
               
               
                   
                 165B 
                 0.4 
               
               
                   
                 166A 
                 52.1 
               
               
                   
                 166B 
                 17.6 
               
               
                   
                 167 
                 3.1 
               
               
                   
                 168 
                 1.4 
               
               
                   
                 169 
                 4.7 
               
               
                   
                 170 
                 0.7 
               
               
                   
                 171 
                 5.2 
               
               
                   
                 172 
                 1.7 
               
               
                   
                 173 
                 1 
               
               
                   
                 174 
                 0.9 
               
               
                   
                 175A 
                 37.1 
               
               
                   
                 175B 
                 9.2 
               
               
                   
                 176A 
                 21.8 
               
               
                   
                 176B 
                 0.9 
               
               
                   
                 176C 
                 98.9 
               
               
                   
                 177 
                 2.1 
               
               
                   
                 178 
                 2.4 
               
               
                   
                 179 
                 8.3 
               
               
                   
                 180A 
                 1.5 
               
               
                   
                 180B 
                 15.3 
               
               
                   
                 181A 
                 3 
               
               
                   
                 181B 
                 71.2 
               
               
                   
                 182 
                 5.4 
               
               
                   
                 183 
                 9.1 
               
               
                   
                 184 
                 1.5 
               
               
                   
                 185 
                 62.5 
               
               
                   
                 186 
                 10.9 
               
               
                   
                 187A 
                 54.2 
               
               
                   
                 187B 
                 19.3 
               
               
                   
                 188 
                 7.3 
               
               
                   
                 189A 
                 0.8 
               
               
                   
                 189B 
                 292 
               
               
                   
                 190 
                 6.6 
               
               
                   
                 191A 
                 0.7 
               
               
                   
                 191B 
                 90.2 
               
               
                   
                 192A 
                 2.1 
               
               
                   
                 192B 
                 2.7 
               
               
                   
                 193 
                 0.6 
               
               
                   
                 194 
                 11.6 
               
               
                   
                 195A 
                 3.3 
               
               
                   
                 195B 
                 2.7 
               
               
                   
                 196A 
                 6.5 
               
               
                   
                 196B 
                 3.1 
               
               
                   
                 196C 
                 7.4 
               
               
                   
                 196D 
                 6.8 
               
               
                   
                 197 
                 3.4 
               
               
                   
                 198 
                 17 
               
               
                   
                 199 
                 6.2 
               
               
                   
                 200 
                 7.1 
               
               
                   
                 201 
                 1.2 
               
               
                   
                 202 
                 5.3 
               
               
                   
                 203 
                 0.3 
               
               
                   
                 204A 
                 21 
               
               
                   
                 204B 
                 8.5 
               
               
                   
                 205A 
                 1.1 
               
               
                   
                 205B 
                 1.9 
               
               
                   
                 206A 
                 1 
               
               
                   
                 206B 
                 0.7 
               
               
                   
                 207A 
                 0.3 
               
               
                   
                 207B 
                 72 
               
               
                   
                 208 
                 0.7 
               
               
                   
                 209 
                 8.9 
               
               
                   
                 210A 
                 0.7 
               
               
                   
                 210B 
                 2.3 
               
               
                   
                 210C 
                 6.9 
               
               
                   
                 211A 
                 25 
               
               
                   
                 211B 
                 0.5 
               
               
                   
                 212A 
                 8 
               
               
                   
                 212B 
                 0.9 
               
               
                   
                 213 
                 28.6 
               
               
                   
                 214A 
                 26.3 
               
               
                   
                 214B 
                 31.2 
               
               
                   
                 214C 
                 0.4 
               
               
                   
                 215A 
                 70 
               
               
                   
                 215B 
                 3.7 
               
               
                   
                 216 
                 0.5 
               
               
                   
                 217 
                 10 
               
               
                   
                 218A 
                 55 
               
               
                   
                 218B 
                 1.1 
               
               
                   
                 219 
                 6.6 
               
               
                   
                 220 
                 2.4 
               
               
                   
                 221 
                 35.4 
               
               
                   
                 222 
                 2.28 
               
               
                   
                 223 
                 2.30 
               
               
                   
                 224 
                 15.49 
               
               
                   
                 225 
                 0.32 
               
               
                   
                 226 
                 2.20 
               
               
                   
                 227A 
                 1.25 
               
               
                   
                 227B 
                 1.16 
               
               
                   
                 228 
                 0.34 
               
               
                   
                 229 
                 2.20 
               
               
                   
                 230 
                 0.84 
               
               
                   
                 231 
                 2.90 
               
               
                   
                 232 
                 0.32 
               
               
                   
                 233 
                 1.40 
               
               
                   
                 234A 
                 0.43 
               
               
                   
                 234B 
                 74.84 
               
               
                   
                 235 
                 0.32 
               
               
                   
                 236A 
                 55.00 
               
               
                   
                 236B 
                 0.96 
               
               
                   
                 236C 
                 2.30 
               
               
                   
                 237A 
                 54.65 
               
               
                   
                 237B 
                 0.19 
               
               
                   
                 238A 
                 1.94 
               
               
                   
                 238B 
                 67.48 
               
               
                   
                 238C 
                 2.32 
               
               
                   
                 238D 
                 2.25 
               
               
                   
                 239 
                 3.60 
               
               
                   
                 240A 
                 91.58 
               
               
                   
                 240B 
                 0.25 
               
               
                   
                 241 
                 9.84 
               
               
                   
                 242 
                 2.28 
               
               
                   
                 243 
                 6.40 
               
               
                   
                 244 
                 6.13 
               
               
                   
                 245 
                 1.60 
               
               
                   
                 246 
                 1.74