Patent Publication Number: US-2022221460-A1

Title: Method of quantifying her2 in breast cancer sample by mass spectrometry and scoring her2 status using the same

Description:
STATEMENT OF GOVERNMENT SUPPORT 
     The invention was made with government support under grant number 20000134 “the Industrial Strategic Technology Development Program” awarded by the Ministry of Trade, Industry, and Energy (MOTIE, Korea) and under grant number HI19C1132 “the Korea Health Industry Development Institute” awarded by the Ministry of Health &amp; Welfare, Republic of Korea. 
    
    
     SEQUENCE LISTING 
     The Sequence Listing submitted in text format (.txt) filed on Sep. 8, 2021, named “SequenceListing.txt”, created on Aug. 26, 2021 (6.19 KB), is incorporated herein by reference. 
     BACKGROUND OF THE INVENTION 
     Field of the Invention 
     The preset disclosure is related to method of quantifying HER2 protein in breast cancer tissue sample by mass spectrometry and its use in companion diagnostics. 
     Description of the Related Art 
     Breast cancer ranks second in the female cancer incidence rate in Korea (14.8%), and the breast cancer incidence rate is 52.1 per 100,000 female population, ranking first among Asian countries. According to the statistics of the Review and Assessment Service, the number of breast cancer patients in Korea increased by about 40,000 over the past five years, and the number of breast cancer-related claims and medical care benefit costs increased by 123.2% and 151.4% in 2017 compared to 2013, respectively. 
     HER2-receptor-positive breast cancer patients account for 15-20% of all breast cancer patients. HER2-receptor-positive breast cancer is more aggressive than other types of breast cancer, and the prognosis is poor due to the high probability of recurrence, and thus the survival rate is low. Therefore, accurately measuring the expression level of HER2 protein in breast cancer patients is very important in determining the prognosis and treatment strategy of HER2 receptor-positive breast cancer patients. 
     However, there is no universal gold standard that can accurately measure the expression level of HER2 protein so far. Analysis and evaluation of HER2 protein and expression using Immunohistochemistry (IHC) and evaluation of HER2 gene amplification using Fluorescent in situ Hybridization (FISH) are FDA-approved methods and the most important technique that has been used so far to determine HER2-positive and negative breast cancers. 
     However, the IHC technique cannot quantitatively measure the expression level of HER2 protein, and depends on the pathologist who determines visually the level of immunostaining of the HER2 protein. Thus, the subjectivity of the pathologist may be involved resulting in the false-positive or false-negative determination leading to a low reproducibility of the determination. When it is found to be HER2 2+(equivocal) through IHC test, the degree of overexpression of HER2 protein is re-evaluated in more detail using FISH, but the FISH technique is difficult and expensive. 
     Therefore, it is necessary to develop a technology to accurately quantify HER2 protein expression levels. 
     US Patent Application Publication No. US 2013/0302328 relates to a method for quantifying full-length HER2 and truncated HER 
     Korean Patent Application Publication No. 2018-0036653 relates to quantification of HER2 protein for optimal cancer treatment. However, the above documents do not provide a method for correcting the differences in the level of HER2 protein expression by patient or patient samples, and the peptide sequence of the epithelial cell-specific protein and the precursor charge status corresponding to the sequence, etc. are not disclosed. 
     SUMMARY OF THE INVENTION 
     The present disclosure is to provide a method for accurately determining the level of HER2 expression in a breast cancer sample using mass spectrometry, and determining a HER2 score based on the expression level. 
     In one aspect of the present disclosure, there is provided method of measuring an expression level of HER2 protein in vitro in a breast cancer sample to provide an information for determining a HER2 score, the method comprising steps of: quantifying or determining an amount of a specific HER2 peptide fragment prepared from the breast cancer sample digested with a protease; and as a normalization factor, determining an amount of any one of the peptide fragments of the protein expressed on epithelial cells and/or a number of tumor cells present in an area of the breast cancer sample; and normalizing the amount of the specific HER2 peptide with the normalization factor to provide a normalized amount of the HER2 peptide; wherein the specific HER2 peptide fragment is VLQGLPR (SEQ ID NO: 1) or FVVIQNEDLGPASPLDSTFYR (SEQ ID NO: 2), wherein the peptide fragments of the protein expressed on the epithelial cells are selected from the Table 1; and wherein the amount of the peptide fragment is determined by a mass spectrometry. 
     In one embodiment of the present disclosure, the breast cancer sample is a FFPE (Formalin-Fixed, Paraffin-Embedded) sample. 
     In other embodiment of the present disclosure, the mass spectrometry includes a tandem mass spectrometry, an ion trap mass spectrometry, a triple quadrupole mass spectrometry, a hybrid ion trap/quadruple mass spectrometry or a time-of-flight mass spectrometry. 
     In still other embodiment of the present disclosure, the mode used for the mass spectrometry is SRM (Selected Reaction Monitoring) or MRM (Multiple Reaction Monitoring). 
     In still other embodiment of the present disclosure, wherein the protease is a trypsin. 
     In still other embodiment of the present disclosure, the method further comprises the step of fractionating the sample digested with a protease or trypsin before it is used for determining the amount of a specific HER2 peptide fragment and the amount of any one of the peptide fragments of the protein expressing on epithelial cells. 
     In still other embodiment of the present disclosure, the step of fractionation is performed by a liquid chromatography. 
     In still other embodiment of the present disclosure, the HER2 peptide is VLQGLPR (SEQ ID NO: 1) or FVVIQNEDLGPASPLDSTFYR (SEQ ID NO: 2), and the normalization factor used is a peptide of VTFLPTGITFK (SEQ ID NO: 3) from JAM1. 
     In still other embodiment of the present disclosure, the method is performed in an MRM mode, and the amount of the normalized HER2 peptide is determined by dividing the peak area ratio of three product ions (y6, 683.4199+; y5, 570.3358+; y4, 442.2772+) fragmented from the peptide VLQGLPR having an intrinsic mass of 391.7478++ by the peak area ratio of three product ions (y9, 1023.5873+; y8, 876.5189+; y7, 763.4349+) fragmented from the JAM1 peptide VTFLPTGITFK having an intrinsic mass of 612.3554++. 
     In still other embodiment of the present disclosure, the method is performed in an MRM mode, and the amount of the normalized HER2 peptide is determined by dividing the peak area ratio of three product ions (y9, 1085.5262+; y8, 998.4942+; b10, 1115.5732+ fragmented from the peptide FVVIQNEDLGPASPLDSTFYR having the intrinsic mass of 790.0655+++ by the peak area ratio of three product ions (y9, 1023.5873+; y8, 876.5189+; y7, 763.4349+) fragmented from the JAM1 peptide VTFLPTGITFK having the intrinsic mass of 612.3554++. 
     In other aspect of the present disclosure, there is provided a method of determining a HER2 score of a breast cancer sample in need thereof using a method according to the present methods disclosed herein, the method comprising the step of correlating the normalized amount of HER2 peptide with a HER2 score: 0, 1+, 2+FISH−, 2+FISH+, or 3+. 
     In one embodiment of the present disclosure, the correlation step includes a) as a control, determining a normalized amount of HER2 peptide of SEQ ID NO:1 or NO:2 from breast cancer samples representing each of the HER2 scores determined by IHC (Immunohistochemistry) and FISH (Fluorescent In Situ Hybridization) to set a threshold value for each the HER2 score; and b) comparing the normalized HER2 peptide amount from the breast cancer sample in need thereof with the threshold value determined in a) to assign the score. 
     In other embodiment of the present disclosure, the threshold value is determined using an Youden index (sensitivity+specificity −1) based on the specificity and sensitivity determined by a ROC curve analysis using the normalized amount of HER2 peptide of a) 
     In other aspect of the present disclosure, there is provided a method of determining a HER2 score of a breast cancer sample in need thereof using a method according to the present methods disclosed herein, the method comprising the step of correlating the normalized amount of HER2 peptide with a HER2 score: 0, 1+, 2+FISH−, 2+FISH+, or 3+. 
     In other embodiment of the present disclosure, the correlation step includes comparing the normalized amount of HER2 peptide with a threshold value determined for each of the HER2 score, wherein the HER2 peptide is the peptide with SEQ ID NO: 1, and the amount of the HER2 peptide is normalized by a normalization factor with SEQ ID NO: 3, wherein the threshold value determined for each of the HER2 score is: 0 for less than −3.4690, 1+ for −3.4690 or more to less than −2.3603, 2+FISH− for −2.3603 or more to less than −1.9241, 2+FISH+ for −1.9241 or more to less than −0.2897, and 3+ for −0.2897 or more, wherein the normalized amount of the HER2 peptide is a value of log 2 of 
     
       
         
           
             
               Peak 
               ⁢ 
               
                   
               
               ⁢ 
               area 
               ⁢ 
               
                   
               
               ⁢ 
               ratio 
               ⁢ 
               
                   
               
               ⁢ 
               of 
               ⁢ 
               
                   
               
               ⁢ 
               HER 
               ⁢ 
               
                   
               
               ⁢ 
               2 
               ⁢ 
               
                   
               
               ⁢ 
               peptide 
               ⁢ 
               
                   
               
               ⁢ 
               
                 ( 
                 
                   SEQ 
                   ⁢ 
                   
                       
                   
                   ⁢ 
                   ID 
                   ⁢ 
                   
                       
                   
                   ⁢ 
                   
                     NO:1 
                   
                 
                 ) 
               
             
             
               Peak 
               ⁢ 
               
                   
               
               ⁢ 
               area 
               ⁢ 
               
                   
               
               ⁢ 
               ratio 
               ⁢ 
               
                   
               
               ⁢ 
               of 
               ⁢ 
               
                   
               
               ⁢ 
               JAM 
               ⁢ 
               
                   
               
               ⁢ 
               1 
               ⁢ 
               
                   
               
               ⁢ 
               
                 peptide 
                 ⁡ 
                 
                   ( 
                   VTFLPTGITFK 
                   ) 
                 
               
             
           
         
       
     
     in which the peak area ratio of the product ion of the HER2 peptide is divided by the peak area ratio of the product ion of the normalization factor. 
     In still other embodiment of the present disclosure, wherein the correlation step includes comparing the normalized amount of HER2 peptide with a threshold value determined for each of HER2 score, wherein the HER2 peptide is the peptide of SEQ ID NO: 2, and the amount of the HER2 peptide is normalized by a normalization factor with SEQ ID NO: 3, wherein the threshold value determined for each of HER2 score is: 0 for less than −1.8208, 1+ for −1.8208 or more to less than −1.2673, 2+FISH− for −1.2673 or more to less than −0.5299, 2+FISH+ for −0.5299 or more to less than 0.9543, and 3+ for −0.9543 or more, wherein the normalized amount of the HER2 peptide is a value of log 2 of 
     
       
         
           
             
               Peak 
               ⁢ 
               
                   
               
               ⁢ 
               area 
               ⁢ 
               
                   
               
               ⁢ 
               ratio 
               ⁢ 
               
                   
               
               ⁢ 
               of 
               ⁢ 
               
                   
               
               ⁢ 
               HER 
               ⁢ 
               
                   
               
               ⁢ 
               2 
               ⁢ 
               
                   
               
               ⁢ 
               peptide 
               ⁢ 
               
                   
               
               ⁢ 
               
                 ( 
                 
                   SEQ 
                   ⁢ 
                   
                       
                   
                   ⁢ 
                   ID 
                   ⁢ 
                   
                       
                   
                   ⁢ 
                   
                     NO:2 
                   
                 
                 ) 
               
             
             
               Peak 
               ⁢ 
               
                   
               
               ⁢ 
               area 
               ⁢ 
               
                   
               
               ⁢ 
               ratio 
               ⁢ 
               
                   
               
               ⁢ 
               of 
               ⁢ 
               
                   
               
               ⁢ 
               JAM 
               ⁢ 
               
                   
               
               ⁢ 
               1 
               ⁢ 
               
                   
               
               ⁢ 
               
                 peptide 
                 ⁡ 
                 
                   ( 
                   VTFLPTGITFK 
                   ) 
                 
               
             
           
         
       
     
     in which the peak area ratio of the product ion of the HER2 peptide is divided by the peak area ratio of the product ion of the normalization factor. 
     Advantageous Effects 
     The method according to the present invention can determine HER2 scores (0, 1+, 2+FISH−, 2+FISH+, 3+) of the breast cancer sample more accurately and reproducibly than conventional methods through accurate quantification of HER2 protein from Formalin-Fixed, Paraffin-Embedded (FFPE) tissue sample of breast cancer. In particular, the present method can distinguish 2+FISH− and 2+FISH+, which so far can only be distinguished by FISH. This makes possible the simple and more accurate breast cancer diagnosis leading to a customized therapy which can extend the lifespan of patients and dramatically reduce overall socio-economic costs. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a schematic diagram of MRM analysis. 
         FIG. 2  is a schematic diagram of a process of classifying HER2 status through quantification of HER2 and JAM1 peptides used as normalization factor from breast cancer FFPE (Formalin-Fixed Paraffin-Embedded) tissue sections using MRM-MS analysis according to one embodiment of the present disclosure. 
         FIG. 3  shows a reverse calibration curve for HER2 (VLQGLPR, y5) and JAM1 (VTFLPTGITFK, y7) according to one embodiment of the present disclosure. Product ions with a wide dynamic range were selected through the reverse calibration curve for the target peptides, and the detection and quantitation limits (LOD, LOQ) and other variables (Linearity, LLOQ, ULOQ) for the product ions were determined. 
         FIG. 4  is a graph showing the peak area ratio of the HER2 peptide product ion according to the reaction time with trypsin in one embodiment of the present disclosure. Since the results of MRM-MS analysis of HER2 product ions may differs depending on the reaction time with trypsin, the reaction time that optimizes the peak area ratio of the HER2 product ions was determined. The optimal reaction time with trypsin was determined as 4 hours. 
         FIG. 5  is a standard curve for the amount of protein extracted per cell number in one embodiment of the disclosure. Because the size of the tumor region in a sample varies from sample to sample, the number of FFPE tissue slides that must be used to extract the same amount of protein also varies from sample to sample. In addition, the greater the difference in the amount of protein extracted from each sample, the greater the difference in peptide yield between the samples. Therefore, in order to ensure that the similar or same amount of protein is extracted from different samples, for a sample with a relatively small tumor region, it is necessary to extract proteins from several consecutive tissue sections. In this context, in order to extract a similar amount of protein from each sample, it is necessary to objectively determine how many slides to use for each sample. Here this was solved by using a standard curve representing the correlation between the number of cells present in the tumor region and the amount of protein extracted from the tumor region. 
         FIG. 6  shows Spearman rank correlation-1 between six HER2 peptides according to one embodiment of the present disclosure. After performing MRM-MS analysis using 210 individual samples and the Agilent 6400 series, correlations between the 6 peptides were determined through Spearman rank correlation. As a result, it was confirmed that there was a positive correlation between the 6 HER2 peptides. 
         FIGS. 7A and 7B  show Spearman rank correlation-2 between six HER2 peptides according to one embodiment of the present disclosure. After performing MRM-MS analysis using 210 individual samples and the Agilent 6400 series, correlations between the 6 peptides were determined through Spearman rank correlation. As a result, it was confirmed that there was a positive correlation between the 6 HER2 peptides. 
         FIG. 8  is a ROC curve showing that HER2 2+FISH− group and the HER2 2+FISH+ group can be distinguished when HER2 quantitative analysis values was normalized with the five normalization factors according to one embodiment of the present disclosure. The HER2 quantitative analysis values was divided by the number of cells in the tumor region, the amount of peptide, the amount of protein, the area of the tumor tissue, or the quantitative analysis value of the epithelial cell-specific peptide or housekeeping peptide to select a normalization factor which is best in distinguishing between HER2 2+FISH− group and the HER2 2+FISH+ group. When the HER2 quantitative analysis values were normalized using the quantitative analysis values of the JAM1 peptide among epithelial cell-specific peptides, it was found that the distinction between the two groups was the best with AUC value of 0.908. 
         FIG. 9  shows the result of distinguishing between HER2 2+FISH− group (61 cases) and the HER2 2+FISH+ group (59 cases) using the HEE2 peptide (VLQGLPR) quantitative analysis value normalized with JAM1 peptide (VTFLPTGITFK) quantitative analysis value. The peak area ratio of the three product ions (y6, 683.4199+; y5, 570.3358+; y4, 442.2772+) fragmented from the HER2 protein peptide (VLQGLPR, 391.7478++) in the range of collision energy of 3.1 to 23.1 normalized by the peak area ratio of the three product ions (y9, 1023.5873+; y8, 876.5189; y7, 763.4349+) fragmented from the epithelial cell-specific protein JAM1 peptide (VTFLPTGITFK, 612.3554++) in the range of collision energy of 10 to 30 was found to be the best in distinguishing HER2 2+FISH−/+ groups with accuracy of 88.33% and AUC of 0.908. This is a result showing that the two groups that cannot be distinguished by a conventional IHC now can be distinguished by the present MRM-MS analysis. 
         FIG. 10  is a result of determining the score over the entire HER2 status using HER2 peptide quantitative analysis values noramlized by JAM1 peptide quantitative analysis values in one embodiment of the present disclosure. For this, the peak area ratio of the three product ions (y6, 683.4199+; y5, 570.3358+; y4, 442.2772+) fragmented from the HER2 protein peptide (VLQGLPR, 391.7478++) in the range of collision energy of 3.1 to 23.1 normalized by the peak area ratio of the three product ions (y9, 1023.5873+; y8, 876.5189; y7, 763.4349+) fragmented from the epithelial cell-specific protein JAM1 peptide (VTFLPTGITFK, 612.3554++) in the range of collision energy of 10 to 30 was used. It was confirmed that all five HER2 status, i.e., HER2 0, 1+, 2+FISH−, 2+FISH+, and 3+, can be distinguished with a significance of p-value &lt;0.01 or p-value &lt;0.001. Based on the normalized HER2 peptide quantitative analysis value, the cutoff value required when HER2 scoring is performed was obtained by converting the normalized HER2 peptide quantitative analysis value to a binomial log (log 2), and then generating an ROC curve to distinguish adjacent HER2 scores and determining the maximum Youden-index (J-index) (J-index=sensitivity+specificity−1). As a result, samples with a binomial logarithmic value of the normalized HER2 peptide quantitative analysis value less than −3.4690 were classified as HER2 0, samples with −3.4690 or more and less than −2.3603 as HER2 1+, samples with −2.3603 or more and less than −1.9241 as HER2 2+FISH−, samples with −1.9241 or more and less than −0.2897 as HER2 2+FISH+, and samples with −0.2897 or more as HER2 3+. 
         FIG. 11  is a result of distinguishing HER2-negative breast cancer group (121 cases) and HER2-positive breast cancer group (89 cases) using HER2 peptide (VLQGLPR) quantitative analysis result normalized by the quantitative analysis result of JAM1 peptide (VTFLPTGITFK) according to one embodiment of the present disclosure. For this, the peak area ratio of the three product ions (y6, 683.4199+; y5, 570.3358+; y4, 442.2772+) fragmented from the HER2 protein peptide (VLQGLPR, 391.7478++) in the range of collision energy of 3.1 to 23.1 normalized by the peak area ratio of the three product ions (y9, 1023.5873+; y8, 876.5189; y7, 763.4349+) fragmented from the epithelial cell-specific protein JAM1 peptide (VTFLPTGITFK, 612.3554++) in the range of collision energy of 10 to 30 was used. This is a significant result with accuracy of 92.9% and AUC of 0.960 in distinguishing HER2-negative and positive breast cancer group. 
         FIG. 12  schematically shows a method of classifying HER2 status through MRM-MS analysis according to the present disclosure. There is a difference between the conventional method using IHC and the present method based on MRM-MS analysis in determining HER2 status. The method of the present invention can distinguish the HER2 2+FISH− group and the HER2 2+FISH+ group, which cannot be distinguished by the conventional method IHC, thus significantly reducing the number of equivocal HER2 2+ groups. Further this can lead to saving the cost and time required for FISH by reducing the absolute number of samples that must be performed, and thus it is expected that the overall cost and time required for HER2 status classification can be saved. 
         FIG. 13  is a result performed to correct the SIS peptide concentration value according to the purity of the SIS peptide which were used for the reverse calibration curve for the VLQGLPR peptide of the HER2 protein and the VTFLPTGITFK peptide of the JAM1 protein of  FIG. 3 . This is to determine the purity of the HER2 (VLQGLPR) and JAM1 (VTFLPTGITFK) SIS peptides used in this study. For HER2 (VLQGLPR) peptide and JAM1 (VTFLPTGITFK) peptide, MRM-MS assay was performed using equal amounts (200 fmol) of unpurified synthetic peptide (heavy, labeled form) and purified synthetic peptides (light, unlabeled form), respectively. Then the purity of the SIS peptide (unpurified synthetic peptide) used in the reverse calibration curve was calculated: 
       
         
           
             
               Purity 
               = 
               
                 
                   
                     Peak 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     area 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     ratio 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     of 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     unpurified 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     synthetic 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     peptide 
                   
                   
                     Peak 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     area 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     ratio 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     of 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     purified 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     synthetic 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     peptide 
                   
                 
                 . 
               
             
           
         
       
       The purity of the HER2 (VLQGLPR) SIS peptide was 44.17%, and the purity of the JAM1 (VTFLPTGITFK) SIS peptide was 44.15%. 
         FIG. 14  is a result of the experiment to evaluate the representativeness of the 10 samples used in  FIG. 5  (standard curve for the amount of protein extracted per cell number) for the entire 210 samples used in this study. There was no statistical difference (P&gt;0.05) found in 5 histopathological features except for the necrosis area (%) between the 10 samples and 210 samples. As to the necrosis area (%), the difference was also found to be negligible with P-value=0.045. Therefore, it can be determined that the 10 samples can represent the entire 210 samples, and it can be considered that the 10 samples are suitable for producing a standard curve for the amount of protein extracted per cell number. 
         FIG. 15  is a ROC curve showing that the present method can distinguish HER2 2+FISH+ and − groups using the peak area ratio of the HER2 peptide normalized by the peak area ratio of the JAM1 (VTFLPTGITFK) peptide, which was selected as an optimal normalization factor in one embodiment of the present disclosure. Each of the peak area ratio of a single HER2 peptide (VLQGLPR), the averaged value of the peak area ratios of the two HER2 peptides (VLQGLPR, GLQSLPTHDPSPLQR) with the highest correlation, and the averaged value of the peak area ratios of the 6 HER2 peptides was normalized by the peak area ratio of the JAM1 (VTFLPTGITFK) peptide, the value of which was then used for ROC curve generation. It was found that the use of the peak area ratio of a single HER2 peptide (VLQGLPR) gave the best result in distinguishing HER2 2+FISH+ and − groups with AUC=0.908 compared to the use of the averaged value of the peak area ratios of several HER2 peptides. 
         FIG. 16  is a result of measuring the stability of the HER2 (VLQGLPR) peptide and the JAM1 (VTFLPTGITFK) peptide in accordance with the Clinical Proteomic Tumor Analysis Consortium (CPTAC) guideline. Peak area ratios of HER2 (VLQGLPR) peptide and JAM1 (VTFLPTGITFK) peptide were determined in low-quality control (QC) and medium-QC samples under six different conditions. (A) The coefficient of variation (CV) value of the peak area ratios of the HER2 (VLQGLPR) peptide and the JAM1 (VTFLPTGITFK) peptide in 6 different conditions in low-QC and medium-QC samples was found to be small with less than 7%, which is below the CV standard of 20%. (B) When it was tested the peak area ratio variability of HER2 (VLQGLPR) peptide and JAM1 (VTFLPTGITFK) peptide in the remaining 5 conditions based on 0 hours in low-QC and medium-QC samples, the recovery was found to be 80%˜120% which is within the standard of the guideline. 
         FIG. 17  is a result of testing the reproducibility of the present HER2 (VLQGLPR) peptide and the JAM1 (VTFLPTGITFK) peptide in accordance with the Clinical Proteomic Tumor Analysis Consortium (CPTAC) guideline. A total of 6 samples of HER2 2+FISH− (n=3) and HER2 2+FISH+(n=3) were tested each day from sample preparation to MRM-MS analysis for 5 consecutive days, and then the variability of the peak area ratios of HER2 (VLQGLPR) and JAM1 (VTFLPTGITFK) peptides were measured. The variability over the 5 days of testing in the HER2 (VLQGLPR) peptide (A) and JAM1 (VTFLPTGITFK) peptide (B) peak area ratios in the 6 samples was found to be small with CV&lt;20%. The values of the HER2 (VLQGLPR) peptide normalized by the JAM1 (VTFLPTGITFK) peptide also showed small variability with a CV&lt;20% in the 5 days of testing. Also, it was found that the normalized value was higher in the HER2 2+FISH+ samples than the three HER2 2+FISH− samples (C). 
     
    
    
     DETAILED DESCRIPTION OF THE EMBODIMENTS 
     In one aspect of the present disclosure, there is provided a method for determining the expression level or the amount of HER2 protein using mass spectrometry in a breast cancer sample, and a method of scoring HER2 status using the same. 
     In one embodiment, the present method relates to measuring the expression level of HER2 of a breast cancer sample in need thereof in vitro for example to provide an information for HER2 status scoring, or for determining the HER2 score of the breast cancer sample based thereon, the method comprising the steps of: determining an amount of a HER2 peptide fragment from a protein preparations prepared from the breast cancer sample; and as a normalization factor, determining an amount of any one of the peptide fragments of the protein expressing on epithelial cells and/or a number of breast cancer cells or tumor cells present in a region of the breast cancer sample; and normalizing the amount of the specific HER2 peptide with the normalization factor to provide a normalized amount of the HER2 peptide, wherein the HER2 peptide fragment is VLQGLPR (SEQ ID NO: 1) or FVVIQNEDLGPASPLDSTFYR (SEQ ID NO: 2), and wherein the peptide fragments of the protein expressing on epithelial cells are selected from the Table 1 below; and wherein the amount of the peptide fragments of HER2 and normalization factor is determined by a mass spectrometry. 
     The mass spectrometry which may be used in the method according to the present disclosure may include tandem mass spectrometry, ion trap mass spectrometry, triple quadrupole mass spectrometry, hybrid ion trap/quadruple mass spectrometry and/or time-of-flight mass spectrometry. In this case, the mass spectrometry mode used may be, for example, Selected Reaction Monitoring (SRM) or Multiple Reaction Monitoring (MRM). 
     In one embodiment, in particular an MRM mode is used. The principle of MRM mass spectrometry is to select a peptide (precursor ion, MS1) having a particular mass to charge (m/z) specific for each target protein after hydrolysis of all selected target proteins into peptides. From the fragments generated when the peptides collide (Quadruple 2, Q2), the fragments (fragmentation ion, MS2) having a particular mass with a specific m/z are selected. Each precursor ion/fragment ion pair obtained from MS1/MS2 is called a transition specific for a target protein (mass fingerprint specific for target protein), and when these transitions are measured for all target proteins (over 300 proteins), the amount of all target proteins present in a sample can be simultaneously quantified relatively or absolutely. For relative or absolute quantification, SIS (having the same amino acid sequence as the corresponding target only with isotopic substitutions) peptide is used as a standard material. In this case, since the amount of the input standard substance (SIS peptide) corresponding to the sample (target) to be measured is known, the amount of target peptide can be proportionally calculated. The transition that passes through MS2 is converted into a digital signal at the detector and converted into a peak chromatogram, and thus by calculating the peak area, the relative and absolute quantitative analysis can be performed. 
     HER2 is a signal receptor protein expressed on epithelial cells, and in general, HER2 receptor plays a role in the control of the growth, division and repair of healthy cells. However, in some cancers, HER2 protein is overexpressed, which also involves the amplification of HER2 gene. When HER2 is overexpressed in certain cancers, it is important to determine the exact expression status of HER2 because a therapeutic agent targeted to HER2 may be considered. The method currently used for determining the HER2 status is IHC (Immunohistochemistry) and FISH (Fluorescent in situ Hybridization), but the process is complicated and particularly time-consuming and expensive. First, the HER2 score is indicated as 0 (negative), 1+(negative), 2+(borderline), or 3+(positive-Her2 protein overexpression) depending on the IHC test results. FISH is used to test whether the HER2 gene is amplified or not, and indicated as amplification positive or amplification negative. FISH is also performed on the borderline samples classified as 2+ to further classify the borderline sample as 2+FISH+ or 2+FISH−. The samples with FISH positive (2+FISH+), and 3+ respond to anti-HER2 therapy. 
     The method according to the present disclosure can accurately determine the status of HER2 without performing FISH. Herein, it was found that 2+FISH− and 2+FISH+, which before only can be distinguished by FISH, can be distinguished accurately by the present method particularly using VLQGLPR (SEQ ID NO: 1) or FVVIQNEDLGPASPLDSTFYR (SEQ ID NO: 2) peptide from the HER2 protein and the peptide from the protein as listed in Table 1 as a standard peptide for normalization with high sensitivity and specificity. The proteins listed in Table 1 can be found by searching UniProt DB (www.uniprot.org) with the ID listed in Table 1. 
     The following peptides and their corresponding m/z, product ions and their corresponding m/z, and ranges of collision energies may be referred to the Examples herein. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 Uniprot 
                 Uniprot 
                   
                   
               
               
                 ID 
                 name 
                 Peptide sequence 
                 
                   
                 
               
               
                   
               
             
            
               
                 Q9Y624 
                 JAM1 
                 VTFLPTGITFK 
                  3 
               
               
                   
               
               
                 P07437 
                 TBB5 
                 ALTVPELTQQVFDAK 
                  4 
               
               
                   
               
               
                 P07437 
                 TBB5 
                 ISVYYNEATGGK 
                  5 
               
               
                   
               
               
                 P18206 
                 VINC 
                 SLGEISALTSK 
                  6 
               
               
                   
               
               
                 P18206 
                 VINC 
                 ELTPQVVSAAR 
                  7 
               
               
                   
               
               
                 P18206 
                 VINC 
                 AIPDLTAPVAAVQAAVSNLVR 
                  8 
               
               
                   
               
               
                 P18206 
                 VINC 
                 AQQVSQGLDVLTAK 
                  9 
               
               
                   
               
               
                 O60716 
                 CTND1 
                 GYELLFQPEVVR 
                 10 
               
               
                   
               
               
                 P51149 
                 RAB7A 
                 VIILGDSGVGK 
                 11 
               
               
                   
               
               
                 P51149 
                 RAB7A 
                 EAINVEQAFQTIAR 
                 12 
               
               
                   
               
               
                 Q15084 
                 PDIA6 
                 TGEAIVDAALSALR 
                 13 
               
               
                   
               
               
                 Q15084 
                 PDIA6 
                 ELSFGR 
                 14 
               
               
                   
               
               
                 P62888 
                 RL30 
                 SLESINSR 
                 15 
               
               
                   
               
               
                 P62888 
                 RL30 
                 LVILANNCPALR 
                 16 
               
               
                   
               
               
                 P62826 
                 RAN 
                 FNVWDTAGQEK 
                 17 
               
               
                   
               
               
                 PO6396 
                 GELS 
                 HVVPNEVVVQR 
                 18 
               
               
                   
               
               
                 P06396 
                 GELS 
                 TGAQELLR 
                 19 
               
               
                   
               
               
                 P55072 
                 TERA 
                 WALSQSNPSALR 
                 20 
               
               
                   
               
               
                 P08708 
                 RS17 
                 VCEEIAIIPSK 
                 21 
               
               
                   
               
               
                 P23528 
                 COF1 
                 NIILEEGK 
                 22 
               
               
                   
               
               
                 P35268 
                 RL22 
                 AGNLGGGVVTIER 
                 23 
               
               
                   
               
               
                 P62277 
                 RS13 
                 LILIESR 
                 24 
               
               
                   
               
               
                 P04406 
                 G3P 
                 GALQNIIPASTGAAK 
                 25 
               
               
                   
               
               
                 P00338 
                 LDHA 
                 VTLTSEEEAR 
                 26 
               
               
                   
               
               
                 P15924 
                 DESP 
                 AELIVQPELK 
                 27 
               
               
                   
               
               
                 P62910 
                 RL32 
                 AAQLAIR 
                 28 
               
               
                   
               
            
           
         
       
     
     In one embodiment, through MRM-MS analysis using FFPE tissue samples from breast cancer patient and Agilent 6400 series, the expression level of HER2 protein may be determined based on the quatitative analysis of the peaks and the peak area ratio of the two proteins, HER2 and JAM1 as follows: the peak of the 3 product ion (y6, 683.4199+; y5, 570.3358+; y4, 442.2772+) fragmented from the HER2 peptide (VLQGLPR, 391.7478++) in collision energy range of 3.1 to 23.1, and the peak of the 3 product ion (y9, 1023.5873+; y8, 876.5189+; y7, 763.4349+) fragmented from JAM1 peptide (VTFLPTGITFK, 612.3554++) of the epithelial cell-specific protein in collision energy range of 10 to 30 to be used as a normalization factor representing the tumor content. Through this, the HER2 status can be determined from 0 to 3+, and the HER2 2+FISH− group and the HER2 2+FISH+ group, which cannot be distinguished by traditional methods, can also be distinguished. Also, based on the HER2 quantitative analysis results, the breast cancer can be classified as either HER2-positive or HER2-negative, which can be used to determine whether to use Herceptin (traszumab) in therapy. 
     In other embodiment, through MRM-MS analysis using FFPE tissue samples from breast cancer patient and Agilent 6400 series, the expression level of HER2 protein may be determined based on the quatitative analysis of the peaks and the peak area ratio of the two proteins, HER2 and JAM1 as follows: the peak of the 3 product ion (y9, 1085.5262+; y8, 998.4942+; b10, 1115.5732+) fragmented from the HER2 peptide (FVVIQNEDLGPASPLDSTFYR, 790.0655+++) in collision energy range of 3.6 to 23.6, and the peak of the 3 product ion (y9, 1023.5873+; y8, 876.5189+; y7, 763.4349+) fragmented from JAM1 peptide (VTFLPTGITFK, 612.3554++) of the epithelial cell-specific protein in collision energy range of 10 to 30 to be used as a normalization factor representing the tumor content. 
     Through this, the HER2 status can be determined from 0 to 3+, and the HER2 2+FISH− group and the HER2 2+FISH+ group, which cannot be distinguished by traditional methods, can also be distinguished. Also, based on the HER2 quantitative analysis results, the breast cancer can be classified as either HER2-positive or HER2-negative, which can be used to determine whether to use Herceptin (traszumab) in therapy. 
     In addition, as a normlization factor, the number of tumor cells contained in the predetermined area of a breast cancer sample was used. For this, in order to correct the differences among the breast cancer samples in the tumor area and tumor cells contained therein, a standard curve is prepared for the amount of extracted protein per tumor cell number, and assign the number of tumor cells in the tumor area into the standard curve to estimate the amount of protein extracted from a predetermined tumor area. Then the number of slides of the breast cancer sample used for analysis is determined in a way to have the similar amount of protein extracted among different samples, enabling more accurate quantitative analysis. 
     In other aspect, the present disclosure is also directed to a method of determining a HER2 score of a breast cancer sample: 0, 1+, 2+FISH−, 2+FISH+, or 3+ using the assay results as described above. 
     According to one embodiment, the method comprises correlating the normalized amount of HER2 peptide with any one of the HER2 score of the breast cancer: 0, 1+, 2+FISH−, 2+FISH+, or 3+, wherein the correlation step, to be used as a control, includes the steps of a) determining a normalized amount of HER2 peptide from breast cancer samples representing each of the HER2 scores for which the HER2 score has been determined by IHC (Immunohistochemistry) and FISH (Fluorescent In Situ Hybridization) so as to set a threshold value for each the HER2 scores; and b) comparing the normalized HER2 peptide amount from the breast cancer sample in need thereof with the threshold value determined in a) to assign the score to the sample in need thereof. 
     In one embodiment, as shown in  FIG. 10 , the threshold value is determined as the value at which the Youden index (Ruopp et al. Biom J. 2008 June; 50(3): 419-430) (J index) becomes maximum (J index=sensitivity+specificity−1) in which the normalized HER2 peptide quantitative analysis value is converted to a binomial log (log 2), and then an ROC curve is generated that distinguishes adjacent HER2 scores to obtain specificity and sensitivity. 
     In one embodiment, when the sequence of SEQ ID NO: 1 is used as a HER2 peptide and a binomial log value of the quantitative analysis values is used, samples having a binomial log value of less than −3.4690 is classified as HER2 0, −3.4690 or more and less than −2.3603 as HER2 1+, −2.3603 or more and less than −1.9241 as HER2 2+FISH−, −1.9241 or more and less than −0.2897 as HER2 2+FISH+, −0.2897 or more as HER2 3+. Further, when the present invention is applied in clinical settings, in addition to the detailed HER2 scoring, it is important to distinguish between a group that requires Herceptin (traszumab) targeted therapy (HER2-positive breast cancer group) and a group that does not (HER2-negative breast cancer group). As shown in  FIG. 11 , based on −1.9241 which is the binary log value of the normalized quantitative analysis value of HER2 peptide, the samples having a value less than −1.9241 can be classified as HER2-negative breast cancer and the sample having a value 1.9241 or more can be classified as HER2-positive breast cancer. 
     In other embodiment, when the sequence of SEQ ID NO: 2 is used as a HER2 peptide and a binomial log value of the quantitative analysis values is used, samples having a binomial log value of less than −1.8208 is classified as HER2 0, −1.8208 or more and less than −1.2673 as HER2 1+, −1.2673 or more and less than −0.5299 as HER2 2+FISH−, −0.5299 or more and less than 0.9543 as HER2 2+FISH+, and 0.9543 or more as HER2 3+. 
     Accordingly, those skilled in the art would be able to perform MRM-MS analysis-based quantitative analysis of the peptides such as HER2 peptides and JAM1 and other normalization peptides disclosed in the present invention, and then determine a threshold value such as shown in  FIG. 11  and determine whether the target based Herceptin (traszumab) therapy is needed. The threshold value may be different depending on the type of HER2 peptides and normalizatoin peptides employed. As mentioned above, the threshold value is determined as the value at which the Youden index (Ruopp et al. Biom J. 2008 June; 50(3): 419-430) index) becomes maximum (J index=sensitivity+specificity—1) in which the normalized HER2 peptide quantitative analysis value is converted to a binomial log (log 2), and then an ROC curve is generated that distinguishes adjacent HER2 scores to obtain specificity and sensitivity. 
     In one embodiment, the sample used in the method according to the present disclosure is FFPE (Formalin-Fixed, Paraffin-Embedded). FFPE tissue samples are the most frequently used clinical samples to diagnose breast cancer and determine whether to apply targeted chemotherapy, and samples in various stages can be stored and easily available at low cost. Therefore, through quatification of HER2 peptided and epithelial cell-specific peptided as a normalization factor by MRM-MS analysis using FFPE tissue samples from breast cancer patients, HER2 scoring can be measured more accurately and reproducibly than conventional methods. 
     In one embodiment, the protein for use in mass spectrometry in the method according to the present invention is digested with a trypsin. In one embodiment, when the FFPE tissue sample used in the method according to the present disclosure, the sample is treated with trypisin particulary for about 4 hours. 
     The present disclosure is further explained in more detail with reference to the following examples. These examples, however, should not be interpreted as limiting the scope of the present invention in any manner. 
     EXAMPLES 
     Materials and Methods 
     1. Clinical Tissue Samples and Characteristics Thereof 
     The FFPE tissue samples used in the present disclosure were collected from a total of 210 breast cancer patients who underwent mastectomy, and the written informed consent was obtained from all the patients. The 210 samples were classified based on their HER2 status as follows: HER2 0 (30 cases), HER2 1+ (30 cases), HER2 2+FISH− (61 cases), HER2 2+FISH+ (59 cases), HER2 3+ (30 cases), and the clinical pathological information for each sample is shown in Table 2. 
     
       
         
           
               
               
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                   
                   
                 HER2 status 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 HER2 2+/ 
                 HER2 2+/ 
                   
               
               
                   
                   
                 HER2 0 
                 HER2 1 
                 (n = 61) 
                 (n = 59) 
                 HER2 3+ 
               
               
                 Group 
                   
                 (n = 30) 
                 (n = 30) 
                 FISH-negative 
                 FISH-positive 
                 (n = 30) 
               
               
                   
               
               
                 Age (years) 
                   
                 53.80 ± 9.57 
                 53.60 ± 11.39 
                 43.62 ± 9.86 
                 54.22 ± 10.95 
                 50.57 ± 11.66 
               
               
                 FISH status 
                   
                   
                   
                   
                   
                   
               
               
                   
                 Negative 
                 0 
                 2 
                 61 
                 0 
                 0 
               
               
                   
                 Positive 
                 0 
                 0 
                 0 
                 59 
                 20 
               
               
                   
                 NA 
                 30 
                 28 
                 0 
                 0 
                 10 
               
               
                 Estrogen Receptor 
                   
                   
                   
                   
                   
                   
               
               
                   
                 Negative 
                 18 
                 7 
                 4 
                 20 
                 20 
               
               
                   
                 Positive 
                 12 
                 23 
                 57 
                 39 
                 10 
               
               
                 Progesterone Receptor 
                   
                   
                   
                   
                   
                   
               
               
                   
                 Negative 
                 21 
                 7 
                 11 
                 30 
                 24 
               
               
                   
                 Positive 
                 9 
                 23 
                 50 
                 29 
                 6 
               
               
                 Subtype 
                   
                   
                   
                   
                   
                   
               
               
                   
                 HER2 
                 0 
                 0 
                 0 
                 21 
                 20 
               
               
                   
                 Luminal A 
                 10 
                 23 
                 53 
                 0 
                 0 
               
               
                   
                 Luminal B 
                 3 
                 0 
                 4 
                 38 
                 10 
               
               
                   
                 TNBC 
                 17 
                 7 
                 4 
                 0 
                 0 
               
               
                 Nuclear grade 
                   
                   
                   
                   
                   
                   
               
               
                   
                 1 
                 0 
                 1 
                 0 
                 1 
                 0 
               
               
                   
                 2 
                 5 
                 15 
                 38 
                 13 
                 5 
               
               
                   
                 3 
                 25 
                 14 
                 72 
                 45 
                 25 
               
               
                   
                 NA 
                 0 
                 9 
                 1 
                 0 
                 0 
               
               
                 Histological grade 
                   
                   
                   
                   
                   
                   
               
               
                   
                 I 
                 1 
                 3 
                 6 
                 1 
                 0 
               
               
                   
                 II 
                 4 
                 16 
                 37 
                 25 
                 8 
               
               
                   
                 III 
                 25 
                 11 
                 17 
                 33 
                 22 
               
               
                   
                 NA 
                 0 
                 0 
                 1 
                 0 
                 0 
               
               
                 Tumor size 
                   
                   
                   
                   
                   
                   
               
               
                   
                 &lt;2.0 cm. 
                 7 
                 12 
                 28 
                 30 
                 19 
               
               
                   
                 2.0-4.9 cm 
                 21 
                 17 
                 32 
                 27 
                 10 
               
               
                   
                 ≥5.0 cm 
                 2 
                 1 
                 1 
                 2 
                 1 
               
               
                   
               
               
                 FISH, fluorescence in situ hybridization 
               
            
           
         
       
     
     2. Treatment of Breast Cancer Tissue Samples and Preparation of FFPE Block. 
     The breast cancer lesion tissues collected from the mastectomy were fixed by placing them in a formalin solution to prevent tissue damage and store for long periods. Before use, the formalin solution on the surface of the tissues were removed by washing the fixed tissues under running water. Subsequently, the tissues were dehydrated by immersing them in water and ethanol in the following order: water→50% ethanol→70% ethanol→85% ethanol→100% ethanol. Next, the tissues were treated with Xylene to remove the dehydrating agent used to facilitate paraffin penetration. Then the tissues were embedeed in paraffin wax. Through this process, the paraffin wax was infiltrated into the tissue and hardened to protect the tissue and the tissue can be stored for long periods. 
     (1) Preparation of Breast Cancer FFPE Tissue Section and H&amp;E Staining 
     FFPE tissue sections were obtained by sectioning FFPE tissue blocks to a thickness of 10 μm using a microtome, and 6 sections were prepared for each sample. One of 6 FFPE tissue sections was used for nuclear and cytoplasmic staining of the cells by H&amp;E (Hematoxylin &amp; Eosin) agent. Then the pathologist examined the H&amp;E-stained FFPE tissue sections and marked the tumor formation site. 
     (2) Deparaffinization, Rehydration and Protein Denaturation of the Breast Cancer FFPE 
     One mg of Rapigest was dissolved in 2104, of 30 mM Tris-Cl (pH 8.5) solution to prepare 0.5% Rapigest solution. The paraffin was removed from the breast cancer FFPE tissue section and rehydrated by immersing the section for 3 mins in the respective solution as follows: Xylene→Xylene→100% ethanol→100% ethanol→85% ethanol→70% ethanol→50% ethanol→water. Then the FFPE tissue sections were treated with 200 μL of 30 mM Tris-Cl (pH 8.5) solution to prevent the tissue from drying out. Then the breast cancer tissue was scraped from the breast cancer FFPE tissue section using a scraper and put into 100 μL of 0.5% Rapigest in an Eppendorf® tube followed by sonication for 10 seconds. Then, it was heated (1,000 rpm, 95° C.) for 30 minutes in a ThermoMixer®. After cool down to room temperature, it was spin-down to move all the solutions to the bottom of the tube. Then the tissues were disrupted using a probe sonicator (Amplitude: 30%, pulses ON and OFF, repeated 20 times for 4 seconds each) and centrifuged at 15,000 rpm for 30 minutes (maintained at 20° C.). After centrifugation, 80 μL of the supernatant was transferred to a new Eppendorf® tube, and the remaining supernatant was transferred to separate Eppendorr tube for BCA analysis. 
     (3) RapiGest™ In-Solution Digeston of Breast Cancer FFPE Tissue Samples 
     Twenty microliter of 200 mM DTT were added to the tube containing 80 μL of the supernatant prepared as above, which was then vortexed to mix the denatured protein with DTT and was spinned down. Then, the tube was heated (1,000 rpm, 60° C.) for 1 hour in a ThermoMixer®. Then 20 μL of 240 mM IAA were added thereto and mixed by vortexing followed by spin-down. Then it was left at room temperature for 1 hour by blocking the light by wrapping it with aluminum foil. Then 60 μL of 0.067 μg/μL trypsin (trypsin:protein=1:50) were added thereto, vortexted and spinned down, after which it was heat treated in ThermoMixer® for 4 hours (1,000 rpm, 37° C.). 
     (4) Remove of RapiGest™ and Preparation of Sample for MRM-MS Analysis 
     Twenty microliter of 30% formic acid were added to the trypsin digested sample as above and vortexed gently, and the acidity was measured using CR paper. Then the sample was incubated in a ThermoMixer® (1,000 rpm, 37° C.) for 30 minutes, and centrifuged for 1 hour (15,000 rpm, 4° C.) to obtain 200 μL of supernatant. Then 100 μL of the supernatant was transferred to a new Eppendorf® tube for MRM-MS analysis, and the remaining 100 μL was transferred to a separate tube for peptide yield measurement by tryptophan fluorescence analysis. 
     For MRM-MS analysis, 90 μL out of 100 μL of the supernatant was transferred into a glass vial, and 10 μL of 150 fmol/μL SIS peptide mixture was added thereto to make a total of 100 μL mixture. The mixture was then vortexed for about 1 minute, and spinned down. 
     3. Capillary Liquid Chromatography for Peptide Separation 
     Prior to MRM-MS analysis, 6 HER2 peptides, 19 epithelial cell-specific peptides and 30 housekeeping peptides were separated by liquid chromatography (1260 Capillary-flow liquid chromatography system; Agilent Technologies, Santa Clara, Calif., USA). 
     For this, impurities (residual RapiGest™ and salt) contained in the sample were removed using a guard column (2.1×15.0 mm, 1.8 μm, 80 Å), and then an analytical column (0.5×35.0 mm, 3.5 μm, 80 Å) was used to perform peptide separation. Solvent A (0.1% formic acid in water) and solvent B (0.1% formic acid in acetonitrile) were used on an LC (liquid chromatography) system consisting of two columns. While flowing 3% solvent B at a flow rate of 40 μL/min for 10 minutes, 40 μL of the sample was injected into the guard column during which the impurities contained in the sample were removed. After switching the valve, the peptides attached to the guard column moved to the analytical column and attached thereto. By flowing solvent B from 3% to 50% gradient at a flow rate of 40 μL/min, the peptides attached to the analytical column were eluted. After washing the column by flowing 60% solvent B at a flow rate of 40 μL/min for 2 minutes, solvent B was flowed from 60% to 3% gradient at a flow rate of 40 μL/min for 1 minute to readjust the column to equilibrium. After equilibration for 4 minutes, the valve was changed to the original position and reconditioning of the guard column and the analytical column occurred simultaneously for 10 minutes. Total sample analysis time was 70 minutes. Then, after injecting the sample into the LC system, the autosampler injector needle and tube were washed with 50% acetonitrile aqueous solution. 
     4. Online Desalting and MRM-MS Analysis 
     MRM-MS quantitative analysis was performed using an Agilent 6400 series. Here, the ionized peptides flowed into Quadrupole 1 (Q1), and a precursor ions with a specific m/z (mass to charge ratio) were selected in Q1. The precursor ions that passed through the Q1 filter were decomposed into product ions due to the fragmentation of the ions by electrical energy in the quadrupole 2 (Q2). The product ions flowed into Quadrupole 3 (Q3), where product ions with a specific m/z were selected, moved to a detector, and converted into a digital signal to appear as a peak chromatogram. Relative and absolute quantitative analysis were then performed by calculating the peak area ( FIG. 1 ). 
     5. Post-Processing of Raw Files and Statistical Analysis 
     The raw file was introduced into the skyline software for post-processing of the raw file derived from LC-MS/MS analysis. In order to calculate quantitative analysis values for 6 HER2 peptides, 19 epithelial cell-specific peptides, and 30 housekeeping peptides, the peak integration for the corresponding peptide peaks was performed (designating the peak corresponding to the retention time of each peptide). The peak area ratio of the SIS peptide (Heavy peptide) peak and the endogenous peptide (Light peptide) peak within the retention time was calculated. For statistical analysis, Mann-Whitney test, area under the receiver operating curve, correlation analysis, etc. were performed using IBM SPSS and MedCalc software and visualized using Prism software. 
     6. Selection of Normalization Factors that can Noramlize Quantitative Analysis Values of HER2 Protein 
     To normalize quantitative data of HER2 peptide, Five normalization factors (tumor tissue area, number of cells in tumor tissue area, total protein amount, total peptide amount, 19 epithelial cell-specific peptides and 30 housekeeping peptides) that can represent tumor contnes were selected. 
     Two of these normalization factors (tumor tissue area and the number of cells in the tumor tissue area) were measured by embedding H&amp;E staining tissue section images in Aperio ImageScope, ver. 12.3.0.5056 (Leica Biosystems, Buffalo Grove, Ill., USA). In Aperio software, after the tumor area was annotated using a pen tool, the area and total cell count of the annotated region were measured with the nuclear counting algorithm. 
     The third normalization factor was the total amount of protein extracted from the tumor region of the 10 μm-thick FFPE tissue section, and the total amount of protein was detemubed by BCA analysis. 
     The fourth normalization factor was the amount of peptide, which is the final product of trypsinization, and the total amount of peptides was determined by tryptophan fluorescence analysis. 
     As a final normalization factor, the peak area ratio (area of light peptide/area of heavy peptide) of 19 epithelial cell-specific peptides and 30 housekeeping peptides quantified by MRM-MS analysis was determined. 
     The normalized HER2 peptide quantitative value is the value obtained by dividing the peak area ratio of the HER2 peptide by the normalization factor, and this value was compared with the IHC and FISH results. 
     The accuracy and AUC values when the (HER2 FISH−) group and the (HER2 FISH+) group can be discriminated were determined using the HER2 peptide quantitative analysis values normalized by five standardization factors. Among them, the normalizaton factor with the highest Accuracy and AUC values was selected as the optimal standardization factor. Then It was used to test that whether the overall HER2 status can be significantly (p&lt;0.05) distinguished using the HER2 peptide level normalized with the optimal standardization factor. 
     Example 1: Flowchart for HER2 Status Classification in MRM-MS Analysis of Clinical Samples 
     For HER2 scoring through MRM-MS analysis, FFPE tissue sections from a total of 210 breast cancer patients were used. HER2 status consists of a total of 5 types (0, 1+, 2+FISH−, 2+FISH+, 3+), and the number of individual samples according to HER2 status was varied (see Table 3). One hundred twenty samples classified as HER2 2+(HER2 equivocal) by IHC were finally determined to be HER2 negative or positive through FISH analysis. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Breast Cancer FFPE tissue (210) 
               
            
           
           
               
               
               
               
               
            
               
                 HER2 (0) 
                 HER2 (1+) 
                 HER2 (2+FISH−) 
                 HER2 (2+FISH+) 
                 HER2 (3+) 
               
               
                   
               
               
                 30 
                 30 
                 61 
                 59 
                 30 
               
               
                   
               
            
           
         
       
     
     Then, the tissue samples collected from the breast cancer patients were made into FFPE blocks, sectioned into consecutive sections with a thickness of 10 μm. One of the FFPE tissue sections was subjected to H&amp;E staining and the image was used for counting the number of cells present in the tumor region using the nuclear counting algorithm of Aperio software. Next, the expected amount of extracted protein was determined by assign the number of cells above to the equation for the standard curve for the amount of protein extracted per numbers of the cells. Based on the calculated amount of protein, the number of FFPE tissue slides to obtain 150 μg of protein was determined. The FFPE tissue sections were deparaffinized and rehydrated, and the proteins were extracted and digested with trypsin to obtain peptides. Then, HER2 peptides and normalization peptides were measured by MRM-MS analysis to finally distinguish the HER2 status (refer to the flowchart of  FIG. 2 ). 
     Example 2: Screening of HER2 Peptides, Epithelial Cell-Specific Peptides and Housekeeping Peptides that can be Detectable from the Breast Cancer FFPE Tissue Samples 
     Through a literature search to select epithelial cell-specific proteins and housekeeping proteins detectable from breast cancer FFPE tissue samples, 46 proteins were selected as a possible normalization proteins from breast cancer FFPE tissue samples. 
     Here it was confirmed through matching analysis with the MS/MS library obtained with the National Institute of Standards and Technology (NIST) that 123 peptides corresponding to a total of 47 proteins including HER2 protein and 46 normalization proteins were detectable by mass spectrometry. 
     Next, in order to select the peptides that are reproducibly detected in MRM-MS analysis, semi-quantitative MRM-MS analysis was performed using a pooled sample of 10 breast cancer FFPE tissues corresponding to HER2 3+. As a results, 37 proteins (62 peptides) were selected as detectable peptides. 
     Based on this, 62 SIS (unpurified stable-isotope-labeled standard) peptides were synthesized, and a mixture of the SIS peptides was prepared. 
     Then, the SIS peptide mixture was spiked into the pooled breast cancer FFPE tissue samples, and MRM-MS was repeated three times to perform the analysis, and using the result, AUDIT (Automated detection of inaccurate and imprecise transitions analysis) was performed to remove erroneously detected ions, and a maximum of three transitions detected with reproducibly high intensity in each peptide were selected. 
     As a result, finally 31 proteins (55 peptides) were selected as final detectable targets through MRM-MS analysis in breast cancer FFPE tissue samples. Among them, 30 normalization proteins (49 peptides) were included (Table 4). 
     
       
         
           
               
               
               
             
               
                 TABLE 4 
               
               
                   
               
               
                 Uniprot 
                 Uniprot 
                   
               
               
                 ID 
                 name 
                 Peptide sequence 
               
               
                   
               
             
            
               
                 P27797 
                 CALR 
                 FVLSSGK 
               
               
                   
                   
                 FYALSASFEPFSNK 
               
               
                   
               
               
                 P23526 
                 COF1 
                 NIILEEGK 
               
               
                   
               
               
                 O60716 
                 CTND1 
                 GYELLFQPEVVR 
               
               
                   
               
               
                 O00571 
                 DDX3X 
                 SFLLDLLNATGK 
               
               
                   
               
               
                 P15924 
                 DESP 
                 AELIVQPELK 
               
               
                   
               
               
                 Q9Y624 
                 JAM1 
                 VTFLPTGITFK 
               
               
                   
               
               
                 P04406 
                 G3P 
                 GALQNIIPASTGAAK 
               
               
                   
                   
                 VPTANVSVVDLTCR 
               
               
                   
               
               
                 P06396 
                 GELS 
                 HVVPNEVVVQR 
               
               
                   
                   
                 TGAQELLR 
               
               
                   
               
               
                 P05783 
                 K1C18 
                 STFSTNYR 
               
               
                   
                   
                 VIDDTNITR 
               
               
                   
                   
                 ASLENSLR 
               
               
                   
               
               
                 P08727 
                 K1C19 
                 ILGATIENSR 
               
               
                   
                   
                 AALEDTLAETEAR 
               
               
                   
               
               
                 P08729 
                 K2C7 
                 SAYGGPVGAGIR 
               
               
                   
                   
                 EVTINQSLLAPLR 
               
               
                   
                   
                 LPDIFEAQIAGLR 
               
               
                   
               
               
                 P05787 
                 K2C8 
                 ISSSSFSR 
               
               
                   
                   
                 ASLEAAIADAEQR 
               
               
                   
               
               
                 P00338 
                 LDHA 
                 VTLTSEEEAR 
               
               
                   
               
               
                 Q96NV8 
                 NECT4 
                 YEEELTLTR 
               
               
                   
               
               
                 P13667 
                 PDIA4 
                 FDVSGYPTLK 
               
               
                   
                   
                 ELSFGR 
               
               
                   
               
               
                 P23264 
                 PPIB 
                 IGDEDVGR 
               
               
                   
               
               
                 P51149 
                 RAB7A 
                 VIILGDSGVGK 
               
               
                   
                   
                 EAINVEQAFQTIAR 
               
               
                   
               
               
                 P62826 
                 RAN 
                 FNVWDTAGQEK 
               
               
                   
               
               
                 P35268 
                 RL22 
                 AGNLGGGVVTIER 
               
               
                   
                   
                 ITVTSEVPFSK 
               
               
                   
               
               
                 P62888 
                 RL30 
                 SLESINSR 
               
               
                   
                   
                 LVILANNCPALR 
               
               
                   
               
               
                 P62910 
                 RL32 
                 AAQLAIR 
               
               
                   
               
               
                 P32969 
                 RL9 
                 TILSNQTVDIPENVDITLK 
               
               
                   
                   
                 GVTLGFR 
               
               
                   
               
               
                 P46783 
                 RS10 
                 IAIYELLFK 
               
               
                   
               
               
                 P62277 
                 RS13 
                 GLTPSQIGVILR 
               
               
                   
                   
                 LILIESR 
               
               
                   
               
               
                 P62249 
                 RS16 
                 GGGHVAQIYAIR 
               
               
                   
               
               
                 P08708 
                 RS17 
                 VCEEIAIIPSK 
               
               
                   
               
               
                 P07437 
                 TBB5 
                 ISVYYNEATGGK 
               
               
                   
                   
                 ALTVPELTQQVFDAK 
               
               
                   
               
               
                 P55072 
                 TERA 
                 WALSQSNPSALR 
               
               
                   
               
               
                 P18206 
                 VINC 
                 AIPDLTAPVAAVQAAVSNLVR 
               
               
                   
                   
                 AQQVSQGLDVLTAK 
               
               
                   
                   
                 SLGEISALTSK 
               
               
                   
                   
                 ELTPQVVSAAR 
               
               
                   
               
            
           
         
       
     
     Example 3: Development (of MRM Analysis) 
     A reverse calibration curves was generated using the pooled 18 HER2 3+ FFPE tissue samples to determine the detection and quantitation limits (LOD, LOQ) and other parameters (Linearity, LLOQ, ULOQ) of the peptides for MRM-MS analysis ( FIG. 3 ). Each point on the calibration curve was generated by performing 3 independent MRM-MS analysis. Linearity, LOD (Limit of detection), LOQ (Limit of quantification), LLOQ (Lower limit of quantification) and ULOQ (Upper limit of quantification were derived from the reverse calibration curve for the y5 product ion of the VLQGLPR peptide of the HER2 protein and the y7 product ion of the VTFLPTGITFK peptide of the JAM1 protein as follows ( FIG. 3 ). 
     The SIS peptide concentration points of the reverse calibration curve for the VLQGLPR peptide of the HER2 protein and the VTFLPTGITFK peptide of the JAM1 protein were adjusted based on the purity of the spiked SIS peptide ( FIG. 13 ), and the values for the y5 product ion of the VLQGLPR peptide of the HER2 protein was as follows: LOD=0.399 fmol, LOQ=0.985 fmol, LLOQ=0.647 fmol, ULOQ=1325 fmol, linear regression value (R2)=0.999 of the equation Y=1.029X−4.732 for the inverse calibration curve. 
     The values for y7 product ion of the VTFLPTGITFK peptide of the JAM1 protein was as follows: LOD=2.212 fmol, LOQ=5.043 fmol, LLOQ=2.587 fmol, ULOQ=1325 fmol, linear regression value (R2)=0.998 of the equation Y=1.113X−3.772 for the inverse calibration curve. 
     The CV range of the 3 independent analysis on the inverse calibration curve of the y5 product ion of the VLQGLPR peptide of the HER2 protein was 4.02% to 19.21%, and the CV range of the 3 independent analysis on the inverse calibration curve of the y7 product ion of the VTFLPTGITFK peptide of the JAM1 protein was 4.91% to 23.45% (Table 5). 
     
       
         
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
             
               
                 TABLE 5 
               
               
                   
               
               
                   
               
               
                 Protein 
                   
                   
                 Cali- 
                 Cali- 
                 Cali- 
                 Cali- 
                 Cali- 
                 Cali- 
                 Cali- 
                 Cali- 
                 Cali- 
                 Cali- 
                 Cali- 
                 Cali- 
               
               
                 Peptide 
                   
                 Blank 
                 brator 
                 brator 
                 brator 
                 brator 
                 brator 
                 brator 
                 brator 
                 brator 
                 brator 
                 brator 
                 brator 
                 brator 
               
               
                 Transition 
                 Measurements 
                 sample 
                 1 
                 2 
                 3 
                 4 
                 5 
                 6 
                 7 
                 8 
                 9 
                 10 
                 11 
                 12 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 HER2 
                 Concentration 
                 N/A 
                 
                   
                 
                 1.29 
                 2.59 
                 5.18 
                 10.36 
                 20.70 
                 41.41 
                 82.82 
                 166.64 
                 331.28 
                 
                   
                 
                 1325.10 
               
               
                 VLQGLPR 
                 point (fmol) 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 y5 
                 Mean Area 
                 0.00 
                 0.03 
                 
                   
                 
                 0.10 
                 0.20 
                 0.40 
                 0.88 
                 
                   
                 
                 3.64 
                 7.03 
                 16.99 
                 28.36 
                 
                   
                 
               
               
                   
                 ratio 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 (Heavy/Light) 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 SD 
                 0.00 
                 0.00 
                 0.01 
                 0.01 
                 0.03 
                 0.02 
                 0.09 
                 0.08 
                 0.44 
                 
                   
                 
                 
                   
                 
                 1.71 
                 5.03 
               
               
                   
                 CV (%) 
                 64.27 
                 
                   
                 
                 18.30 
                 
                   
                 
                 16.92 
                 4.02 
                 10.22 
                 5.22 
                 11.9   
                 19.21 
                 4.46 
                 6.02 
                 7.80 
               
               
                 JAM1 
                 Concentration 
                 N/A 
                 
                   
                 
                 5.17 
                 10.35 
                 20.70 
                 41.39 
                 
                   
                 
                 165.56 
                 331.13 
                 662.25 
                 1324.50 
                 N/A 
                 N/A 
               
               
                 VTFLPTGITFK 
                 point (fmol) 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 y7 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 N/A 
                 N/A 
               
               
                   
                 Mean Area 
                 
                   
                 
                 0.23 
                 0.49 
                 0.80 
                 1.94 
                 5.09 
                 11.46 
                 
                   
                 
                 44.51 
                 113.44 
                 204.75 
                 N/A 
                 N/A 
               
               
                   
                 ratio 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 (Heavy/Light) 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 SD 
                 0.04 
                 
                   
                 
                 0.11 
                 0.18 
                 
                   
                 
                 0.60 
                 1.48 
                 4.03 
                 
                   
                 
                 5.57 
                 33.24 
                 N/A 
                 N/A 
               
               
                   
                 CV (%) 
                 57.75 
                 
                   
                 
                 23.45 
                 21.92 
                 17.55 
                 11.88 
                 12.95 
                 19.13 
                 
                   
                 
                 4.91 
                 
                   
                 
                 N/A 
                 N/A 
               
               
                   
               
               
                 SD, standard deviation; 
               
               
                 CV, coefficient of variation; 
               
               
                 N/A, Not available. 
               
               
                     indicates data missing or illegible when filed 
               
            
           
         
       
     
     Example 4: Determination of Optimal Incubation Time with Trypsin 
     Since the MRM-MS analysis result of the HER2 peptides may vary depending on the reaction time with trypsin, the trypsin reaction time test was performed as follows to select an optimal reaction time with trypsin that can produce optimal strength of the HER2 peptide detected in MRM-MS analysis. 
     The trypsin reaction time for the experiment was set to 8 points (1, 2, 4, 8, 12, 16, 20, 24 h), and because at least 100 μg of protein is required for each point, one HER2 3+ FFPE tissue sample with a relatively large tumor tissue area (from which about 200 μg of protein may be extracted) from each of 6 cases was treated with 0.5% RapiGest™ in solution digestion. After digestion, 10 μL of each sample was pooled, and the concentration was measured through BCA analysis. After sequential reaction with DTT and IAA using 80 μL of the supernatant of each of the 6 samples, 60 μL of 0.067 μg/μL trypsin was added (trypsin:protein=1:50) to each tube, vortexed and spin-down was performed. Six samples were then pooled into one tube and then aliquoted in the same volume to eight separate tubes for digestion with trypsin. The trypsin reaction time (1, 2, 4, 8, 12, 16, 20, 24 hours) was set differently for each of the 8 tubes, and the trypsin reaction was stopped at the end of each time set, and then formic acid was added to a final concentration of 3% to remove RapiGest™. Eight samples were mixed with SIS peptide (90 μL of sample+10 μL of SIS peptide), and 10 μL of each was pooled to prepare a sample. This is to determine the retention time through analysis of the pooled sample, and to analyze the sample according to the trypsin reaction time by scheduled MRM-MS. 
     The pooled samples were analyzed once by unscheduled MRM-MS. After inserting the raw file into Skyline, peak integration was performed to determine the retention time for each peptide. After preparing the method file for scheduled MRM-MS analysis, 8 individual samples according to the trypsin reaction time were subjected to scheduled MRM-MS analysis (analyzed twice). Among the product ions of the six HER2 peptides, one or two product ions with good detection for each peptide were selected and the peak area ratio (L/H) value was calculated. After comparing the results of each trypsin reaction time, the final optimal reaction time with trypsin was determined to be 4 hours ( FIG. 4 ). 
     Example 5: Generation of a Standard Curve for the Amount of Extracted Protein Per Number of Cells 
     Because the size of the tumor site varies from sample to sample, the number of FFPE tissue slides that need to be used to extract the same amount of protein from different samples also varies from sample to sample. In addition, the greater the difference in the amount of protein extracted from each sample, the greater the difference in peptide yield between the samples. Therefore, in order to ensure that the same/similar amount of proteins are extracted from each samples, it is necessary to extract proteins from several consecutive tissue sections for the samples with a relatively small tumor area. 
     In this context, in order to extract a similar/same amount of protein from each sample, it is necessary to objectively determine how many slides to use for each sample for the assay. This was solved by using a standard curve indicating the correlation between the number of cells present in the tumor region and the protein extracted from the tumor region. 
     Based on the hypothesis that the amount of extracted protein is proportional to the number of cells, proteins was extracted from each of 10 individual samples with different cell numbers in the tumor area in which 3 replicates of FFPE tissue slides were used for each sample. The number of cells in the tumor area of individual FFPE tissue samples was determined by applying H&amp;E staining tissue section images in Aperio software, in which the tumor area was marked using the pen tool, and then the Nuclear counting algorithm was used to determine the number of cells in the tumor tissue area. 
     Then, the amount of protein in a total of 30 samples was measured by BCA analysis. As a result, it was confirmed that the amount of extracted protein per cell number was linear (R2=0.9916), and the CV between the three replicates at 10 points was all less than 20% (Table 6,  FIG. 5 ). 
     The 10 individual samples used to generate a standard curve for the amount of protein extracted per number of cells was found to be representative of the total 210 samples used in this study. This was confirmed by that there were no statistically significant differences between the 10 samples and 210 samples in the six histopathological characteristics—tumor area (μm), cell number, intratumoral fat content (%), necrosis area (%), tumor content (%), and tumor-infiltrating lymphocyte (%) ( FIG. 14 ). 
     
       
         
           
               
               
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                   
                   
                 Extracted proteins (μg) 
               
            
           
           
               
               
               
               
               
            
               
                 No. 
                 Cell counts 
                 Average 
                 SD 
                 CV (%) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 1 
                 48968 
                 9.00 
                 1.30 
                 14.49 
               
               
                 2 
                 69469 
                 12.49 
                 0.41 
                 3.25 
               
               
                 3 
                 120075 
                 25.34 
                 2.92 
                 11.54 
               
               
                 4 
                 149723 
                 31.59 
                 0.23 
                 0.72 
               
               
                 5 
                 185695 
                 34.12 
                 3.07 
                 9.00 
               
               
                 6 
                 228840 
                 68.83 
                 6.44 
                 9.84 
               
               
                 7 
                 379759 
                 92.50 
                 7.46 
                 8.07 
               
               
                 8 
                 552041 
                 148.54 
                 6.80 
                 4.58 
               
               
                 9 
                 736601 
                 165.51 
                 3.89 
                 2.35 
               
               
                 10 
                 1915542 
                 406.13 
                 25.28 
                 6.23 
               
               
                   
               
               
                 SD, Standard deviation; 
               
               
                 CV, Coefficient of variation 
               
            
           
         
       
     
     Example 6: Results of MRM-MS Analysis of HER2 Peptides, Epithelial Cell-Specific Peptides, and Housekeeping Peptides in 210 Individual Samples 
     Six HER2 peptides, 19 epithelial cell-specific peptides and 30 housekeeping peptides selected in the previous Example were quantitatively analyzed by MRM-MS analysis of 210 individual samples using Agilent 6400 series. 
     As a result of determining the correlation between the six HER2 peptides through Spearman rank correlation, it was confirmed that there was a positive correlation between the six HER2 peptides ( FIGS. 6, 7A and 7B ). The peptide LLDIDETEYHADGGK and the peptide ELVSEFSR showed the lowest Spearman correlation coefficient (ρ) of 0.596. The peptides VLQGLPR, GLQSLPTHDPSPLQR and FVVIQNEDLGPASPLDSTFYR showed a high Spearman correlation coefficient (ρ) of 0.8 or more. All six peptides are from the same HER2 protein, and thus theoretically, the results of MRM-MS quantitative analysis from the peptides should be similar to each other. In this context, the correlation between the quantitative analysis results of six HER2 peptides of the present invention was expressed as a Spearman correlation coefficient, and since all correlations had positive values, it can be seen that the quantitative analysis results from the peptides are similar to each other. The above results showed the correlation results between HER2 peptides quantified in 210 samples analyzed here. Theoretically, the correlation coefficient between the six peptides should be close to 1.000, but since the characteristics of each peptide are different, the correlation between the quantified values by MRM-MS analysis may not be the theoretically expected 1.000. Referring to  FIG. 6 , in the case of the ELVSEFSR peptide, the correlation with the other five peptides was found to be relatively low. This means that the yield of the ELVSEFSR peptide was different from that of the other 5 peptides during the sample preparation process (for example, the ELVSEFSR peptide produced a yield lower than the other 5 peptides), or the ELVSEFSR peptide has a different degree of ionization than that of other peptides in MRM-MS analysis. For example, this means that the ELVSEFSR peptide has a lower peptide yield during sample pretreatment than the other five peptides, and the ionization is not good during MRM-MS analysis thus lowering the detection efficiency during analysis. For this reason, it was determined that the ELVSEFSR peptide is not suitable for HER2 scoring in HER2 quantitative analysis. On the other hand, the VLQGLPR peptide showed a high correlation coefficient (ρ) of 0.8 or more with GLQSLPTHDPSPLQR and FVVIQNEDLGPASPLDSTFYR, and also showed a relatively high Spearman correlation coefficient (ρ) with the other three peptides and thus selected as HER2 analysis peptide. 
     Example 7: Agreement Between MRM-MS Data and IHC/FISH Data 
     Including four normalization factors that can represent tumor content and peak area ratio (L/H) of the peptides from MRM-MS analysis (peak area ratio of epithelial cell-specific peptides and housekeeping peptides (L/H)), the quantitative values of the six HER2 peptides were divided by a total of five normalization factors to normalize the quantitative values of the six HER2 peptides. 
     Then, using the normalized quantitative values of the six HER2 peptides, a statistical analysis was performed to determine Accuracy and AUC value of the present invention in which the statistical analysis was performed as to distinguish between the HER2 2+FISH− group and the HER2 2+FISH+ group, which cannot be distinguished by the conventional method (HER2 status scoring based on IHC). 
     Each of the MRM-MS quantitative analysis values of 6 HER2 peptides (MRM analysis values of the peptides) was divided by 53 normalization factors (number of cells in tumor area, tumor tissue area, protein amount, peptide amount, 49 epithelial cell-specific peptides and housekeeping peptide), and the value was used to determine the Accuracy and AUC values for a total of 318 combinations. Among these 318 combinations, the top 30 combinations with an AUC value of 0.800 or higher are shown in Table 7. 
     AUC values for the 318 combinations ranged from 0.617 to 0.908. When the quantitative analysis result of HER2 peptide was normalized by various normalization factors, it was found that the ability to distinguish between the HER2 2+FISH− group and the HER2 2+FISH+ group was found to be excellent in the order of epithelial cell-specific peptide and housekeeping peptide, the number of cells in the tumor area, peptide amount, protein amount, and tumor tissue area ( FIG. 8 ). 
     Among them, it was found that the normalized value, in which the peak area ratio of the three product ions (y6, 683.4199+; y5, 570.3358+; y4, 442.2772+) produced from the fragmentation of the HER2 peptide (VLQGLPR, intrinsic mass value 391.7478++) in a collision energy range of 3.1˜23.1 was normalized by the peak area ratio of the three product ions (y9, 1023.5873+; y8, 876.5189+; y7, 763.4349+) produced from the fragmentation of the epithelial cell-specific protein JAM1 peptide (VTFLPTGITFK, intrinsic mass value 612.3554++) in a collision energy range of 10-30, was the most accurate in distinguishing the HER2 2+FISH− group and the HER2 2+FISH+ group as an Accuracy of 88.33% and AUC of 0.908 ( FIG. 9 ). Among 61 HER2 2+FISH− samples, 54 (88.52%) were correctly classified as HER2 negative, and among 59 HER2 2+FISH+ samples, 52 (88.14%) were correctly classified as HER2 positive. Overall, among 120 equivocal HER2 samples, 113 (88.33%) were correctly classified (Table 8). 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 7 
               
               
                   
               
               
                   
                 HER2 Peptide 
                   
                 AUROC 
                   
               
               
                 Number 
                 sequence 
                 Normalization factor 
                 value 
                 95% Cl 
               
               
                   
               
             
            
               
                  1 
                 VLQGLPR 
                 *PAR of JAM1_ VTFLPTGITFK 
                 0.908 
                 0.842-0.953 
               
               
                   
               
               
                  2 
                 VLQGLPR 
                 PAR of TBB5_ ALTVPELTQQVFDAK 
                 0.867 
                 0.793-0.922 
               
               
                   
               
               
                  3 
                 VLQGLPR 
                 PAR of VINC_ SLGEISALTSK 
                 0.854 
                 0.778-0.912 
               
               
                   
               
               
                  4 
                 VLQGLPR 
                 PAR of VINC_ ELTPQVVSAAR 
                 0.853 
                 0.777-0.911 
               
               
                   
               
               
                  5 
                 VLQGLPR 
                 PAR of CTND1_ GYELLFQPEVVR 
                 0.852 
                 0.775-0.910 
               
               
                   
               
               
                  6 
                 VLQGLPR 
                 PAR of VINC_ AIPDLTAPVAAVQAAVSNLVR 
                 0.848 
                 0.771-0.907 
               
               
                   
               
               
                  7 
                 VLQGLPR 
                 PAR of TBB5_ ISVYYNEATGGK 
                 0.844 
                 0.767-0.904 
               
               
                   
               
               
                  8 
                 VLQGLPR 
                 PAR of VINC_ AQQVSQGLDVLTAK 
                 0.842 
                 0.754-0.902 
               
               
                   
               
               
                  9 
                 VLQGLPR 
                 PAR of RAB7A_ VIILGDSGVGK 
                 0.836 
                 0.757-0.897 
               
               
                   
               
               
                 10 
                 VLQGLPR 
                 PAR of PDIA6_ TGEAIVDALSALR 
                 0.832 
                 0.752-0.894 
               
               
                   
               
               
                 11 
                 VLQGLPR 
                 PAR of RAB7A_ EAINVEQAFQTIAR 
                 0.831 
                 0.752-0.894 
               
               
                   
               
               
                 12 
                 VLQGLPR 
                 PAR of RL30_ SLESINSR 
                 0.831 
                 0.752-0.893 
               
               
                   
               
               
                 13 
                 VLQGLPR 
                 PAR of RAN_ FNVWDTAGQEK 
                 0.830 
                 0.750-0.892 
               
               
                   
               
               
                 14 
                 VLQGLPR 
                 PAR of GELS_ HVVPNEVVVQR 
                 0.828 
                 0.749-0.891 
               
               
                   
               
               
                 15 
                 VLQGLPR 
                 PAR of PDIA6_ ELSFGR 
                 0.827 
                 0.747-0.890 
               
               
                   
               
               
                 18 
                 VLQGLPR 
                 PAR of TERA_ WALSQSNPSALR 
                 0.826 
                 0.745-0.889 
               
               
                   
               
               
                 17 
                 VLQGLPR 
                 PAR of RS17_ VCEEIAIIPSK 
                 0.824 
                 0.744-0.888 
               
               
                   
               
               
                 18 
                 VLQGLPR 
                 PAR of COF1_NIILEEGK 
                 0.822 
                 0.742-0.888 
               
               
                   
               
               
                 19 
                 VLQGLPR 
                 PAR of RL22_ AGNLGGGVVTIER 
                 0.817 
                 0.736-0.882 
               
               
                   
               
               
                 20 
                 FVVIQNEDLGPALDSTFYR 
                 PAR of JAM1_ VTFLPTGITFK 
                 0.816 
                 0.735-0.881 
               
               
                   
               
               
                 21 
                 VLQGLPR 
                 PAR of RS13_ LILIESR 
                 0.814 
                 0.733-0.880 
               
               
                   
               
               
                 22 
                 VLQGLPR 
                 PAR of G3P_ GALQNIIPASTGAAK 
                 0.813 
                 0.732-0.878 
               
               
                   
               
               
                 23 
                 VLQGLPR 
                 PAR of LDHA_ VTLTSEEEAR 
                 0.813 
                 0.731-0.878 
               
               
                   
               
               
                 24 
                 VLQGLPR 
                 Total cell counts 
                 0.810 
                 0.728-0.875 
               
               
                   
               
               
                 25 
                 VLQGLPR 
                 PAR of RL30_ LVILANNCPALR 
                 0.809 
                 0.727-0.875 
               
               
                   
               
               
                 26 
                 VLQGLPR 
                 PAR of GELS_ TGAQELLR 
                 0.805 
                 0.722-0.871 
               
               
                   
               
               
                 27 
                 FVVIQNEDLGPALDSTFYR 
                 PAR of CTND1_ GYELLFQPEVVR 
                 0.804 
                 0.722-0.871 
               
               
                   
               
               
                 28 
                 VLQGLPR 
                 PAR of DESP_ AELIVQPELK 
                 0.803 
                 0.721-0.870 
               
               
                   
               
               
                 29 
                 VLQGLPR 
                 PAR of RL32_ AAQLAIR 
                 0.808 
                 0.720-0.870 
               
               
                   
               
               
                 30 
                 VLQGLPR 
                 Total peptide amount (μg) 
                 0.802 
                 0.719-0.869 
               
               
                   
               
               
                 *PAR = Peak area ratio 
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                   
                 TABLE 8 
               
             
            
               
                   
                   
               
               
                   
                 Equivocal 
                 Gold standard (IHC/FISH) 
               
            
           
           
               
               
               
               
            
               
                   
                 HER2 cases 
                 Positive 
                 Negative 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 MRM- 
                 Positive 
                 52 
                 7 
               
               
                   
                 MS 
                 Negative 
                 7 
                 54 
               
               
                   
                   
               
               
                   
                 Percent of cases correctly classified : 88.33% 
               
            
           
         
       
     
     Example 8: Classification of HER2 Status Using Normalized HER2 Peptide Quantitative Analysis Values Determined by Mass Spectrometry 
     The normalized value in which the peak area ratio of the three product ions (y6, 683.4199+; y5, 570.3358+; y4, 442.2772+) produced by fragmentation of the HER2 protein peptide (VLQGLPR, 391.7478++) in a collision energy range of 3.1 to 23.1 divided by the peak area ratio of three product ions (y9, 1023.5873+; y8, 876.5189+; y7, 763.4349+) produced by fragmentation of the epithelial cell specific JAM1 peptide (VTFLPTGITFK, 612.3554++) in a collision energy range of 10 to 30 was used to classify the HER2 status of all samples through Mann Whitney U test statistical analysis. 
     When HER2 scoring is performed based on the normalized HER2 peptide quantitative analysis value, the cutoff value that can be used as a criterion to distinguish the status was determined as follows: first the normalized HER2 peptide quantitative analysis value was converted to a binomial log (log 2), and then an ROC curve was generated that distinguishes adjacent HER2 scores to obtain specificity and sensitivity, and the Youden&#39;s J statistic (Ruopp et al. Biom J. 2008 June; 50(3):419-430) was used to calculate the value at which the Youden index (J index) becomes the maximum (J index=sensitivity+specificity−1). As a result, samples with a binomial log value of the normalized HER2 peptide quantitative analysis value of less than −3.4690 was classified as HER2 0, −3.4690 or more and less than −2.3603 as HER2 1+, and −2.3603 or more and less than −1.9241 as HER2 2+FISH. −1.9241 or more and less than −0.2897 as HER2 2+FISH+, and −0.2897 or more as HER2 3+. 
     As a result, it was confirmed in the present disclosure that a total of five HER2 status, i.e., HER2 0, 1+, 2+FISH−, 2+FISH+, 3+ can be distinguished with a significance of p-value &lt;0.05 ( FIG. 10 ). Each of 210 samples used herein were separately scored as HER2 0, 1+, 2+FISH, 2+FISH+, or 3+ through IHC and FISH analysis. It was found that the score determined by the conventional methods were in perfect agreement with the status determined by the present method. 
     In addition, when the present invention is applied in clinical settings, together with a detailed HER2 scoring, it is also important to distinguish between a group that requires Herceptin (traszumab) targeted therapy (HER2-positive breast cancer group) and a group that does not (HER2-negative breast cancer group). It was found that the normalized HER2 quantitative analysis values of the present method can distinguish between the HER2 negative group (HER2 0, 1+, 2+FISH−) and the HER2 positive group (HER2 2+FISH+, 3+) with statistical significance of p-value &lt;0.05 ( FIG. 11 ). As shown in  FIG. 11 , the binomial log value −1.9241 of the normalized quantitative analysis value of HER2 peptide can be used as a criterion to effectively distinguish HER2-negative (less than −1.9241) and positive (−1.9241 or more) breast cancers. 
     These results indicates that the present method can be effectively used to determine whether to use Herceptin (traszumab) targeted therapy in clinical settings by performing MRM-MS analysis-based quantitative analysis of HER2 peptide and JAM1 peptide followed by calculation of the cutoff values as shown in  FIGS. 10 and 11  to determine HER2 score. 
     The present method has an advantage over the conventional method in HER2 scoring. The present invention can accurately discriminate between HER2 2+FISH− and HER2 2+FISH+, which cannot be distinguished by the conventional IHC, thereby remarkably reducing the number of HER2 2+ equivocals. This also can lead to the reduction of the cost and time required for FISH by reducing the absolute number of samples that must be tested by FISH, and overall cost and time reduction for HER2 status classification can be achieved ( FIG. 12 ). 
     Example 9: Comparison of Single HER2 Peptide Peak Area Ratio and HER2 Multi-Peptide Peak Area Ratio Average Value in Distinguishing Equivocal HER2 Group, and Comparison of Single-Marker Analysis and Multi-Marker Analysis 
     The peak area ratio of the HER2 peptide normalized by the peak area ratio of the JAM1 (VTFLPTGITFK) peptide as an optimal normalization factor was tested as to distinguishing between the HER2 2+FISH− group and the HER2 2+FISH+ group. For this, a total of three peak area ratios of the HER2 peptide were used as follows: (1) the peak area ratio of a single HER2 (VLQGLPR) peptide, and (2) the average peak area ratio of the two HER2 peptides (VLQGLPR, GLQSLPTHDPSPLQR) with the highest correlation with a Spearman correlation coefficient (ρ) of 0.858, and (3) the average peak area ratio of six HER2 peptides (VLQGLPR, GIWIPDGENVK, LLDIDETEYHADGGK, ELVSEFSR, FVVIQNEDLGPASPLDSTFYR, GLQSLPTHDPSPLQR). As a result, the HER2 2+FISH− group and the HER2 2+FISH+ group was distinguished as follows: (1) AUC value 0.908 (95% CI, 0.842-0.953) when the peak area ratio of a single HER2 (VLQGLPR) peptide was used having the best result in distinguishing the HER2 2+FISH− and HER2 2+FISH+ groups, and (2) AUC value was 0.858 (95% CI, 0.788-0.928) when the average peak area ratio of the two HER2 peptides (VLQGLPR, GLQSLPTHDPSPLQR) with the highest correlation with a Spearman correlation coefficient (ρ) of 0.858 having the next best result in distinguishing the HER2 2+FISH− and HER2 2+FISH+ groups. And, (3) AUC value of 0.827 (95% CI, 0.752-0.903) when the average peak area ratio of 6 HER2 peptides (VLQGLPR, GIWIPDGENVK, LLDIDETEYHADGGK, ELVSEFSR, FVVIQNEDLGPASPLDSTFYR, GLQSLPTHDPSPLQR) was used in distinguishing HER2 2+FISH− and HER2 2+FISH+ group. Taken together, this indicates that the single HER2 peptide (VLQGLPR) can distinguish between the HER2 2+FISH− and HER2 2+FISH+ groups with AUC value=0.908, better than using the average value of the peak area ratios of several HER2 peptides ( FIG. 15 ). 
     In distinguishing between the HER2 2+FISH− group and the HER2 2+FISH+ group using the normalized peak area ratio of the HER2 peptide, the diagnostic performance of the peak area ratio of the HER2 peptide normalized by classical statistical analysis was tested. Among 120 equivocal HER2 samples, a total of 60 randomly selected samples (30 HER2 2+FISH−, 30 HER2 2+FISH+) were used for the training set. Single-marker analysis and multi-marker analyses were performed by logistic regression analysis to determine the best predictive model from each. AUC value was generated by 5-fold cross validation in the training set. 
     In the single-marker analysis, the peak area ratio of the three product ions (y6, 683.4199+; y5, 570.3358+; y4, 442.2772+) fragmented from the HER2 protein peptide (VLQGLPR, intrinsic mass 391.7478++) in the range of collision energy of 3.1˜23.1 normalized by the peak are ratio of the three product ions (y9, 1023.5873+; y8, 876.5189; y7, 763.4349+) fragmented from the epithelial cell-specific protein JAM1 peptide (VTFLPTGITFK, intrinsic mass 612.3554++) in collision energy in the range of 10 to 30 was found to be the best predictive model. In the training set, it showed that AUC=0.950, sensitivity=93.3%, specificity=93.3%, and in the test set, it showed that AUC=0.891, sensitivity=82.8%, specificity=90.3% (Table 9). 
     In multimarker analysis, the following three marker combination was found to be the best predictive model: (1) the peak area ratio of the three product ions (y6, 683.4199+; y5, 570.3358+; y4, 442.2772) fragmented from HER2 protein peptide (VLQGLPR, intrinsic mass value 391.7478++) in collision energy in the range of 3.1 to 23.1 normalized by the peak area ratio of the three product ions (y9, 1023.5873+; y8, 876.5189+; y7, 763.4349+) fragmented from the epithelial cell-specific protein JAM1 peptide (VTFLPTGITFK, intrinsic mass value 612.3554++) in collision energy in the range of 10 to 30, (2) Normalized peak area ratio of HER2 protein peptide (ELVSEFSR) with the peak area ratio of RS16 protein peptide (GGGHVAQIYAIR), and (3) Normalized the peak area ratio of the HER2 protein peptide (VLQGLPR) with the peak area ratio of the VINC protein peptide (SLGEISALTSK). In the training set, it showed AUC=0.969, sensitivity=93.3%, specificity=93.3%, and in the test set, it showed AUROC=0.899, sensitivity=76.7%, specificity=96.7% (Table 9). 
     
       
         
           
               
               
               
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                   
                   
                 Single-marker analysis 
                 Multi-marker analysis 
               
            
           
           
               
               
               
               
               
               
            
               
                 Group 
                   
                 Training set 
                 Test set 
                 Training set 
                 Test set 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 HER2 2+FISH− 
                 AUROC 
                 0.950 
                 0.891 
                 0.969 
                 0.899 
               
               
                 vs 
                 Sensitvity 
                 0.933 
                 0.828 
                 0.933 
                 0.767 
               
               
                 HER2 2+FISH+ 
                 Specificity 
                 0.933 
                 0.903 
                 0.933 
                 0.967 
               
               
                   
               
               
                 AUROC, area under the reciever operating curve. 
               
               
                 Single marker, the light-to-heavy peptide peak area ratio (PAR) for the HER2 surrogate 
               
               
                 peptide (VLQGLPR), normalized by that for the JAM1 surrogate peptide (VTFLPTGITFK). 
               
               
                 Multi-marker; (1) the light-to-heavy peptde PAR for the HER2 surrogate peptide (VLQGLPR), normalized by that for the JAM1 surrogate peptide (VTFLPTGITFK), 
               
               
                 (2) the light-to-heavy peptide PAR for the HER2 surrogate peptide (ELVSEFSR). normalized by that for the RS16 surrogate peptide (GGGHVAQIYAIR), and 
               
               
                 (3) the light-to-heavy peptide PAR for the HER2 surrogate peptide (VLQGLPR), normalized by that for the VINC surrogate peptide (SLGEISALTSK). 
               
            
           
         
       
     
     Example 10: Evaluation of Stability and Reproducibility According to CPTAC Guideline of HER2 (VLQGLPR) Peptide and JAM1 (VTFLPTGITFK) Peptide 
     The results are shown in  FIGS. 16 and 17 , Tables 10 and 11 (stability, reproducibility). 
     The stability and reproducibility of the three product ions (y6, 683.4199+; y5, 570.3358+; y4, 442.2772+) fragmented from HER2 protein peptide (VLQGLPR, intrinsic mass 391.7478++) in the collision energy in the range of 3.1 to 23.1 and the three product ions (y9, 1023.5873+; y8, 876.5189+; y7, 763.4349+) fragmented from JAM1 peptide (VTFLPTGITFK, intrinsic mass 612.3554++) in the collision energy in the range of 10 to 30 were evaluated according to the Clinical Proteomic Tumor Analysis Consortium (CPTAC) guidelines. 
     For the stability evaluation of the HER2 (VLQGLPR) peptide and JAM1 (VTFLPTGITFK) peptide according to the CPTAC guideline, the low-quality control (QC) samples and the medium-QC samples each in 6 different storage conditions were analyzed in MRM-MS analysis and then tested whether the target peptides can be stably analyzed in MRM-MS analysis. MRM-MS analysis under 6 conditions: (1) MRM-MS analysis at 4° C. immediately after sample preparation (0 hours), (2) MRM-MS analysis after storing the sample at 4° C. for 6 hours, (3) MRM-MS analysis after storing the sample at 4° C. for 24 hours, (4) MRM-MS analysis by freezing and thawing the sample at 80° C. (Batch 2), (5) MRM-MS analysis by freezing and thawing the sample twice at 80° C. (Batch 3), (6) MRM-MS analysis after thawing the sample stored at 80° C. for 4 weeks (Batch 4). 
     The variability in the peak area ratio of the HER2 (VLQGLPR) and JAM1 (VTFLPTGITFK) peptides of the low-QC sample and the medium-QC sample under six storage conditions was small and all within 7%, which falls within the CPTAC CV % guideline of 20% (A of  FIG. 16 , Table 10). The variability in the peak area ratio of the HER2 (VLQGLPR) peptide and the JAM1 (VTFLPTGITFK) peptide under the remaining five storage conditions at 0 hour in the low-QC sample and the medium-QC sample is 80% to 120% in the recovery which falls within the CPTAC guideline (&lt;±20% (B of  FIG. 16 , Table 10). 
     The reproducibility evaluation of HER2 (VLQGLPR) peptide and JAM1 (VTFLPTGITFK) peptide according to the CPTAC guideline is to evaluate the reproducibility of the entire MRM-MS assay workflow. For the HER2 2+FISH− (n=3), and HER2 2+FISH+(n=3) samples, the tests from sample preps to MRM-MS analysis were performed everyday for 5 days and the variability of the peak area ratio of the HER2 (VLQGLPR) peptide and the JAM1 (VTFLPTGITFK) peptide were measured. The variability over 5 days of the HER2 (VLQGLPR) peptide ( FIG. 17 , A, Table 11) and JAM1 (VTFLPTGITFK) peptide ( FIG. 17 , B, Table 11) peak area ratios in 6 samples was small as CV&lt;20%. The values of the HER2 (VLQGLPR) peptide normalized to the JAM1 (VTFLPTGITFK) peptide also showed small variability with a CV&lt;20% for 5 days. The normalized value was found to be higher in the HER2 2+FISH+ sample than in the HER2 2+FISH− sample ( FIG. 17 , C, Table 11), indicating that the present method can accurately determine the expression level of HER2 protein. 
     
       
         
           
               
               
               
               
               
               
               
               
               
               
               
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 Target Information 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 Uniprot 
                   
                 Quanti- 
                   
                   
                   
                 Comparing with 
               
               
                 Gene 
                 Accession 
                 Peptide 
                 fication 
                 Batch 1 
                 CV (%) 
                 Batch 1 
                 Time Zero (%) 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 Symbol 
                 Number 
                 Sequence 
                 ion a   
                 0 h 
                 6 h 
                 24 h 
                 Batch 2 
                 Batch 3 
                 Batch 4 
                 6 h 
                 24 h 
                 Batch 2 
                 Batch 3 
                 Batch 4 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Low QC 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 ERBB2 
                 P04626 
                 VLQGLPR 
                 2.y5.1 
                 1.41 
                 2.04 
                 2.12 
                 1.91 
                 2.38 
                 2.06 
                 107.60 
                 106.67 
                 107.33 
                 105.02 
                 109.25 
               
               
                 F11R 
                 Q9Y624 
                 VTFLPTGITFK 
                 2.y7.1 
                 4.10 
                 2.37 
                 4.41 
                 1.83 
                 3.84 
                 3.30 
                 106.03 
                 101.14 
                  93.05 
                  93.26 
                 103.45 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Medium QC 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 ERBB2 
                 P04626 
                 VLQGLPR 
                 2.y5.1 
                 6.96 
                 4.46 
                 5.00 
                 4.17 
                 5.32 
                 5.54 
                  98.76 
                 
                   
                 
                  98.62 
                 102.93 
                 109.03 
               
               
                 F11R 
                 Q9Y624 
                 VTFLPTGITFK 
                 2.y7.1 
                 4.25 
                 4.37 
                 4.50 
                 2.52 
                 3.01 
                 3.77 
                 102.2   
                 101.34 
                  90.8   
                 
                   
                 
                  96.02 
               
               
                   
               
               
                 QC, quality control; 
               
               
                 CV, coefficient of variation. 
               
               
                   a The quantification ion column presents the following quanitities separated by periods precursor ion charge, production ion type, and product ion charge. 
               
               
                     indicates data missing or illegible when filed 
               
            
           
         
       
     
     
       
         
           
               
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Target information 
                 Inter-day CV (%) 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Uniprot 
                   
                   
                   
                 Quanti- 
                 Samples 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Gene 
                 Accession 
                 Uniprot 
                   
                 Peptide 
                 fication 
                 HER2 2+FISH− 
                 HER2 2+FISH+ 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 Symbol 
                 Number 
                 ID 
                 Protein Name 
                 Sequence 
                 ion a   
                 1 
                 2 
                 3 
                 1 
                 2 
                 3 
               
               
                   
               
               
                 ERBB2 
                 P04626 
                 ERB32 
                 Receptor tyrosine-protein kinase erbB-2  
                 VLQGLPR 
                 2.y5.1 
                 1.14 
                 2.20 
                 3.42 
                 4.72 
                 1.89 
                 1.61 
               
               
                 F11R 
                 Q9Y624  
                 JAM1 
                 Junctional adhesion molecule A 
                 VTRPTGHTK  
                 2.y7.1 
                 3.11 
                 11.17 
                 5.11 
                 12.93 
                 6.09 
                 0.47 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Normalized HER2 level (PAR of VLQGLPR.y5 / PAR of VTFLPTGITFK.y7) 
                 3.1    
                 10.41  
                    .30 
                 8.27 
                 5.00 
                 1.86 
               
               
                   
               
               
                 QC, quality control; 
               
               
                 CV, coefficient of variation. 
               
               
                   a The quantification ion column presents the following quanitities separated by periods precursor ion charge, production ion type, and product ion charge. 
               
               
                     indicates data missing or illegible when filed 
               
            
           
         
       
     
     Example 11: Comparison of Time and Cost of MRM-MS Assay and IHC/FISH Method when Measuring HER2 Status for 30 Samples 
     Here we compared objectively how superior the MRM-MS assay developed in the present invention is in terms of time and cost saved compared to IHC/FISH, a traditional method for evaluating HER2 status. Assuming that the HER2 status of 30 samples was measured, it was confirmed that the MRM-MS assay took 3.4 times less time and 4.6 times less costly than IHC/FISH. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                   
                 MRM-MS assay 
                 IHC 
                 FISH 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Parameter 
                 Time (h) 
                 Cost (USD) 
                 Time (h) 
                 Cost (USD) 
                 Time (h) 
                 Cost USD) 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 Assay format 
                 Liquid chromatearaphy-mass 
                 Antibody-based 
                 Gene amplification assay 
               
            
           
           
               
               
               
               
               
            
               
                   
                 spectrometry 
                 immunochistochemical assay 
                   
                   
               
            
           
           
               
               
               
               
            
               
                 Specimen type 
                 FFPE tissue 
                 FFPE tissue 
                 FFFE tissue 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 The number of FFPE 
                   
                   
                   
                   
                   
                   
               
            
           
           
               
               
               
               
            
               
                 tissue sections for 30 
                 80 
                 30 
                 30 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 samples 
                   
                   
                   
                   
                   
                   
               
               
                 Sample preparation 
                 15.5 
                 1053.54 
                 4.15 
                 356.13 
                 52 
                 5,007.51 
               
               
                 Consumable items 
                 NA 
                 78.58 
                 NA 
                 23.97 
                 NA 
                 35.83 
               
               
                 Analytical run (or 
                 6 (12 min/1 
                 51.81 (1.73/1 
                 2.5 (5 min/1 
                 NA  
                 15 (30 min/1 
                 NA 
               
               
                 IHC/FISH imaging) 
                 analytical run) 
                 analytical run)  
                 interpretation) 
                   
                 interpretation) 
                   
               
               
                 Total 
                 21.5 
                 1183.93 
                 6.65 
                 380.10 
                 67 
                 5,043.34 
               
               
                   
               
               
                 MRM-MS, multiple reaction monitoring-mass spectrometry; 
               
               
                 Et-OH. ethanol; 
               
               
                 DTT, dithiothreitol; 
               
               
                 IAA, iodoacetamide; 
               
               
                 FA formic acid; 
               
               
                 LC, liquid chromatography; 
               
               
                 Ab, antibody; 
               
               
                 DAB, 3.3&#39;-Diaminobenzidine; 
               
               
                 PBS, phosphate-buffered saline; 
               
               
                 SSC, saline sodium citrate buffer. 
               
               
                 The estimated time and costs are based an the analysis of 30 samples, The IHC and FISH procedures are based on the protocol used in actual clinical practice. Because only 15 samples can be performed simultaneously using FISH, the estimated time corresponding to each step is 2-fold that required for 15 samples. The cost units are provided a USD, based on the exchange rate of KRW to USD in February of 2020. The prices for other consumables or reagents that did not significantly impact the total experimental costs were excluded. The labor costs and the operating costs were also excluded, due to variability depending on the tape of instrument. 
               
            
           
         
       
     
     The various singular/plural permutations may be expressly set forth herein for sake of clarity. Although a few embodiments of the present disclosure have been shown and described, it would be appreciated by those skilled in the art that changes may be made in this embodiment without departing from the principles and sprit of the invention, the scope of which is defined in the claims and their equivalents. 
     Unless defined or interpreted otherwise, all technical and scientific terms and any acronyms used herein have the same meanings as commonly understood by one of ordinary skill in the art in the field of the invention. The contents of all publications disclosed as references herein are incorporated herein by reference.