Patent Publication Number: US-2020282047-A1

Title: Epstein-barr virus vaccines

Description:
BACKGROUND 
     Epstein-Barr virus (EBV), also referred to as human herpesvirus 4, is one of the most common human viruses worldwide. Ninety five percent of adults are infected with this virus. EBV spreads most commonly through bodily fluids, primarily saliva, and is the primary cause of infectious mononucleosis (“mono”) and other illnesses. Seventy five percent of college students (18-22 years) with primary EBV infection will develop mono. Symptoms of EBV can include fatigue, fever, inflamed throat, swollen lymph nodes in the neck, enlarged spleen, swollen liver, and rash. While many people are infected with EBV in childhood, childhood symptoms are not distinguished from other mild, brief childhood illnesses. Typically, only teenagers and adults exhibit symptoms characteristic of EBV infection, and although recover is about two to four weeks, some people may feel fatigued for several weeks or even months. Following an EBV infection, the virus becomes latent and, in some cases, may be reactivated. Those with weakened immune systems are more likely to develop symptoms if EBV is reactivated. Currently, there is no vaccine to prevent primary infection or disease. 
     SUMMARY 
     Provided herein, in some embodiments, are Epstein-Barr virus (EBV) ribonucleic acid (RNA) (e.g., mRNA) vaccines (e.g., combination vaccines) that elicit potent neutralizing antibodies and robust T cell responses, inhibit the production of viral immunomodulatory factors, and/or prevent viral latency. In some aspects, the EBV vaccines include a RNA having an open reading frame (ORF) encoding an EBV antigen, wherein intramuscular (IM) administration of a therapeutically effective amount of the vaccine to a subject induces in the subject a neutralizing antibody titer and/or a T cell immune response (e.g., a CD4+ and/or a CD8+ T cell immune response). 
     In some embodiments, the neutralizing antibody titer is at least 100 (e.g., at least 500, or at least 1000) NT 50  following, for example, a single dose (e.g., a single 10 μg-200 μg dose) of an EBV RNA vaccine. In some embodiments, the neutralizing antibody titer is at least 100 (e.g., at least 500, or at least 1000) NT 50  following a booster (second) dose of an EBV RNA vaccine. 
     In some embodiments, the neutralizing antibody titer is sufficient to reduce EBV infection of B cells by at least 50% (e.g., by at least 60%, 70%, 80% or 90%), or relative to a neutralizing antibody titer of an unvaccinated control subject or relative to a neutralizing antibody titer of a subject vaccinated with a live attenuated EBV vaccine, an inactivated EBV vaccine, or a protein subunit EBV vaccine. 
     In some embodiments, the neutralizing antibody titer is induced in the subject following fewer than three (one or two) doses of the vaccine. 
     In some embodiments, a single dose of an EBV RNA vaccine is of 10 μg-100 μg. 
     In some embodiments, the neutralizing antibody titer and/or a T cell immune response is sufficient to reduce the rate of symptomatic infectious mononucleosis relative to the neutralizing antibody titer of unvaccinated control subjects. 
     In some embodiments, the neutralizing antibody titer and/or a T cell immune response is sufficient to reduce the rate of asymptomatic EBV infection relative to the neutralizing antibody titer of unvaccinated control subjects. 
     In some embodiments, the neutralizing antibody titer and/or a T cell immune response is sufficient to prevent EBV latency the subject. 
     In some embodiments, the neutralizing antibody titer and/or a T cell immune response is sufficient to reduce chronic fatigue in the subject. 
     In some embodiments, the neutralizing antibody titer is sufficient to block fusion of EBV with epithelial cells and/or B cells of the subject. 
     In some embodiments, the neutralizing antibody titer is induced within 20 days following a single 10-100 μg of the vaccine. In some embodiments, the neutralizing antibody titer is induced within 40 days following a second 10-100 μg dose of the vaccine. 
     In some embodiments, the ability of a vaccine of the present disclosure to induce a neutralizing antibody response can be demonstrated by injecting animals, e.g., mice or non-human primates, with the vaccine and testing the ability of serum from the animal to neutralize the ability of the virus to infect human B cells. 
     In some embodiments, the T cell immune response comprises a CD4 +  T cell immune response. In some embodiments, the T cell immune response comprises a CD8 +  T cell immune response. In some embodiments, the T cell immune response comprises both a CD4 +  T cell immune response and CD8 +  T cell immune response. 
     In some embodiments, after vaccination, the EBV antigen is expressed on the surface of cells of the subject. In some embodiments, the ability of the vaccine to be expressed can be tested in a model system, e.g., a mouse or non-human primate model. In some embodiments, the ability of the vaccine to be expressed can be tested in vitro, e.g., using human cells. 
     In some embodiments, a single 2 μg dose of the vaccine induces in mice NT 50  neutralizing antibody titers of about 100. In some embodiments, a 2 μg booster dose of the vaccine induces in mice NT 50  neutralizing antibody titers of about 1000. 
     In some embodiments, the EBV vaccine comprises a RNA having an ORF encoding two EBV antigens, or two RNAs, each having an ORF encoding an EBV antigen. 
     In some embodiments, the vaccine comprises a RNA having an ORF encoding two (at least two) EBV antigens formulated in a lipid nanoparticle. In some embodiments, the vaccine comprises two (at least two) RNAs, each having an ORF encoding an EBV antigen, wherein the two RNAs are formulated in a single lipid nanoparticle. In some embodiments, the vaccine comprises two RNAs, each having an ORF encoding an EBV antigen, wherein the each RNAs is formulated in a separate lipid nanoparticle. 
     In some embodiments, the EBV vaccines further include at least one (e.g., 2, 3, 4, 5 or more) additional RNA having an ORF encoding at least one (e.g., 2, 3, 4, 5 or more) additional EBV antigen. 
     In some embodiments, the lipid nanoparticle comprises a molar ratio of 20-60% ionizable cationic lipid, 5-25% non-cationic lipid, 25-55% sterol, and 0.5-15% PEG-modified lipid 
     In some embodiments, the EBV antigens are selected from the group consisting of: gp350, gH, gL, gB, gp42, LMP1, LMP2, EBNA1, and EBNA3. 
     In some embodiments, the EBV antigen is a gH-gL fusion, whereby gH is linked to gL through a linker, such as a GGGGS linker. In some embodiments, the GGGGS linker comprises three GGGGS motifs (SEQ ID NO: 224). In some embodiments, the GGGGS linker comprises four GGGGS motifs (SEQ ID NO: 225)). In some embodiments, the EBV RNA comprises the nucleotide sequence of SEQ ID NO: 218. In some embodiments, the EBV RNA comprises the nucleotide sequence of SEQ ID NO: 221. 
     In some embodiments, the EBV antigens include EBV gp350 antigen, EBV gH antigen, and EBV gL antigen. In some embodiments, the EBV antigens further include EBV gp42 antigen and/or gB antigen. 
     In some embodiments, the EBVgp350 antigen is a wild-type EBV gp350 antigen, a mutated EBV gp350 antigen, or a truncated EBV gp350 antigen. 
     In some embodiments, the EBV antigens are selected from the EBV antigens listed in the Sequence Listing. 
     In some embodiments, the EBV antigens (one or more EBV antigens) are fused to a scaffold moiety. In some embodiments, the scaffold moiety is selected from the group consisting of: ferritin, encapsulin, lumazine synthase, hepatitis B surface antigen, and hepatitis B core antigen. 
     In some embodiments, the RNA comprises messenger RNA (mRNA). 
     In some embodiments, the RNA further comprises a 5′ UTR. In some embodiments, the 5′ UTR comprises a sequence identified by SEQ ID NO: 1 or SEQ ID NO: 104. 
     In some embodiments, the RNA further comprises a 3′ UTR. In some embodiments, the 3′ UTR comprises a sequence identified by SEQ ID NO: 3 or SEQ ID NO: 106. 
     In some embodiments, the EBV antigen is fused to a signal peptide. In some embodiments, the signal peptide is a bovine prolactin signal peptide, optionally comprising SEQ ID NO: 115. 
     In some embodiments, the RNA is unmodified. 
     In some embodiments, the RNA comprise at least one modified nucleotide. In some embodiments, at least 80% (e.g., 90% or 100%) of the uracil in the ORF comprise 1-methyl-pseudouridine modification. 
     Also provided herein, in some aspects, are methods that include administering to a subject an EBV vaccine of the present disclosure in a therapeutically effective amount to induce in the subject a neutralizing antibody titer and/or a T cell immune response. 
     In some embodiments, efficacy of the EBV vaccine is at least 80% (e.g., 85%, 90%, 95%, 98% or 100%) relative to unvaccinated control subjects. 
     In some embodiments, detectable levels of EBV antigen are produced in the serum of the subject at 1-72 hours post administration of the vaccine. 
     In some embodiments, a neutralizing antibody titer of at least 100 (e.g., at least 500 or at least 1000) NU/ml is produced in the serum of the subject at 1-72 hours post administration of the vaccine. 
     In some embodiments, the therapeutically effective amount is a total dose of 20 μg-200 μg (e.g., 50 μg-100 μg). 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1A  shows data from a flow cytometry analysis of indel-free codon-optimized glycoprotein 350 (gp350) variant surface expression in HeLa cells using 72A1 antibody.  FIGS. 1B and 1C  are bar graphs showing percent gp350 variant expression (percent 72A1 positive) on the surface of HeLa cells transfected with 1 μg ( FIG. 1B ) or 0.5 μg ( FIG. 1C ) of mRNA. 
         FIG. 2A  shows data from a flow cytometry analysis of expression of gp350 mRNA having one of two different 5′ untranslated region (UTR) sequences (compare UTR A and UTR B).  FIG. 2B  is a bar graph showing percent gp350 expression on the surface of HeLa cells transfected with 0.5 μg of a gp350 mRNA having one of the two different 5′ UTR sequences. 
         FIG. 3  is a graph showing the geometric mean (with 95% confidence interval) of neutralizing antibody titers produced in Balb/c mice following intramuscular (IM) vaccination with mRNA encoding EBV gp350 variants formulated in a lipid nanoparticle. A 2 μg dose was administered on Day 1 and then again on Day 21. Mice were bled on Day 21 and Day 43. NT 50  titers represent the reciprocal serum dilutions to block 50% viral entry. All gp350 variants exhibited comparable neutralizing titers. 
         FIG. 4A  shows data from a flow cytometry analysis of surface expression of the indicated EBV antigens (EBV gp42) and EBV antigen complexes (EBV gH/gL/gp42 with indicated 5′ UTR, or EBV gH/gp42) in HeLa cells using 2D4 antibody.  FIG. 4B  is a bar graph showing percent antigen expression (percent 2D4 positive) on the surface of HeLa cells transfected with 0.25 μg of the mRNA 24 hours post transfection. 
         FIG. 5  is a graph showing the geometric mean (with 95% confidence interval) of neutralizing antibody titers produced in Balb/c mice following IM vaccination with mRNA encoding indicated EBV antigens (EBV gp350) and EBV antigen complexes (EBV gH/gL/gp42 or gH/gL/gp42/gp350) formulated in a lipid nanoparticle. Various indicated doses were administered on Day 1 and then again on Day 29. Mice were bled on Day 28 and Day 57. NT 50  titers represent the reciprocal serum dilutions to block 50% viral entry. These data show that gp350 and gH/gL/gp42 elicit comparable B cell neutralizing titers. There is no interference observed by combining all of the mRNAs in one LNP vaccine. 
         FIG. 6A  shows data from a flow cytometry analysis of surface expression of EBV gH antigen and EBV gH/gL antigen complex in HeLa cells using 2A8 antibody, or surface expression of EBV gH/gL antigen complex in HeLa cells using 2D4 antibody.  FIG. 6B  is a bar graph showing percent gH/gL expression on the surface of HeLa cells transfected with 0.25 μg of a gH mRNA and a gL mRNA, having one of the two different 5′ UTR sequences, 24 hours post transfection. 
         FIG. 7  is a graph showing gH/gL-specific binding antibody titers (log 10 ) produced in Balb/c mice following IM vaccination with mRNA encoding indicated EBV antigen complexes (EBV gH/gL, EBV gH/gL/gB, EBV gH/gL/gp350, or EBV gH/gL/gB/gp350) formulated in a lipid nanoparticle. Various indicated doses were administered. 
         FIG. 8  is a graph showing gB-specific binding antibody titers (log 10 ) produced in Balb/c mice following IM vaccination with mRNA encoding indicated EBV antigen (EBV gB) or EBV antigen complexes (EBV gH/gL/gB, EBV gB/gp350, or EBV gH/gL/gB/gp350) formulated in a lipid nanoparticle. Various indicated doses were administered. 
         FIG. 9  is a graph showing gp350-specific binding antibody titers (log 10 ) produced in Balb/c mice following IM vaccination with mRNA encoding indicated EBV antigen (EBV gp350) or EBV antigen complexes (EBV gH/gL/gp350, EBV gB/gp350, or EBV gH/gL/gB/gp350) formulated in a lipid nanoparticle. Various indicated doses were administered. 
         FIGS. 10A-10D  show antigen-specific CD8 T cell responses to various EBV latent genes in Balb/c mice following IM vaccination with mRNA encoding either LMP1, LMP2, EBNA1 (EBAN1d1-400), EBNA3A alone or a combination (combo) of LMP1, LMP2, EBNA1, EBNA3A and gp350. 
         FIGS. 11A-11D  show antigen-specific CD4 T cell responses to various EBV latent genes in Balb/c mice following IM vaccination with mRNA encoding either LMP1, LMP2, EBNA1 (EBAN1d1-400), EBNA3A alone or a combination (combo) of LMP1, LMP2, EBNA1, EBNA3A and gp350. 
         FIG. 12  shows schematics of EBV gp350 variants of the present disclosure. 
         FIGS. 13A-13B  show data from a flow cytometry analysis for surface expression in HeLa cells transfected with mRNA encoding either a linked glycoprotein L (gL) and glycoprotein H (gH) construct (gL-gH Linker A or gL-gH Linker B) or individual gH and gL that are co-transfected using 2A8 antibody.  FIG. 13B  is a bar graph showing percent gL-gH expression (percent 2A8 positive and percent CL40 positive) on the surface of the HeLa cells transfected with the indicated mRNA. Mean fluorescence intensity (MFI) is also shown. 
         FIG. 14  shows EBNA1-specific polyclonal CD4 and CD8 T cell responses (e.g., IFNγ, TNFα, and IL-2 secretion) in Balb/c mice following IM vaccination with various mRNAs encoding EBV antigens (LMP2, EBNA1, gH, gL, and gp350, with UTR1 or UTR2) or mRNA encoding EBNA1 antigen alone. 
         FIG. 15  shows LMP2-specific polyclonal CD4 and CD8 T cell responses (e.g., IFNγ, TNFα, and IL-2 secretion) in Balb/c mice following IM vaccination with mRNAs encoding various EBV antigens (LMP2, EBNA1, gH, gL, and gp350, with UTR1 or UTR2) or mRNA encoding LMP2 antigen alone. 
         FIG. 16  shows a schematic of the experimental protocol (top) and graphs of the resulting gp350-specific IgG titers (bottom left) and gH/gL-specific IgG titers (bottom right) in immune sera of non human primates (NHPs) vaccinated with a combination EBV mRNA vaccine (gp350, gH, gL, LMP2, and EBNA1) or control. 
         FIG. 17  is a graph showing the neutralizing titers against EBV infection of Raji B cells in immune sera of NHPs vaccinated with the indicated doses and constructs or the neutralizing titers present in EBV+ human sera. 
         FIG. 18  is a graph showing the gp350-specific IgG titers following cell transfection with EBV vaccine constructs generated using different downstream purification processes. 
         FIG. 19  is a graph showing the gH/gL-specific IgG titers following cell transfection with EBV vaccine constructs generated using different downstream purification processes. 
     
    
    
     DETAILED DESCRIPTION 
     The Epstein-Barr virus (EBV) is a double-stranded DNA γ-herpesvirus that infects B cells and epithelial cells, causing infectious mononucleosis, and that has been linked to malignancies, such as Burkitt&#39;s lymphoma, Hodgkin&#39;s lymphoma, and nasopharyngeal carcinoma, in both cell types in vivo. Nearly 95% of the population is infected by EBV by adulthood and carries EBV DNA throughout life. EBV is maintained in a latent state in infected B lymphocytes, with periodic reactivation of lytic replication. 
     EBV, widespread in all human populations, can be isolated in vitro via its ability to transform resting human B cells into permanent lymphoblastoid cell lines (LCLs) expressing the virus-coded antigens EBNA1, 2, 3A, 3B, 3C, and LP and the latent membrane proteins (LMPs) 1, 2A, and 2B. EBV isolates can be categorized as type 1 or type 2 on the basis of marked allelic polymorphisms within the EBNA2, 3A, 3B, and 3C genes and into distinct strains on the basis of more-subtle sequence variations within the EBNA1, EBNA2, and LMP1 genes and certain lytic cycle genes. 
     EBV has three glycoproteins, glycoprotein B (gB), gH, and gL, that form the core membrane fusion machinery mediating viral entry into a cell. The gH and gL proteins associate to form a heterodimeric complex, which is necessary for efficient membrane fusion and is also implicated in direct binding to epithelial cell receptors required for viral entry. EBV uses different pathways for the infection of epithelial cells and B lymphocytes. For both cell types, the minimal viral glycoprotein components that mediate membrane fusion have been identified. As with other herpesviruses, EBV uses the core viral entry glycoproteins, glycoprotein B (gB) and the gH/gL complex. For the infection of B lymphocytes, EBV requires an additional protein, gp42, which binds to host HLA class II molecules, triggering the membrane fusion step. gp42 has multiple functional sites for interaction with gH/gL, HLA class II, and potentially, another unknown binding ligand that could be engaged through a large surface-exposed hydrophobic pocket. The gp42 protein binds to the gH/gL complex with nanomolar affinity through its N-terminal region, and this interaction can be recapitulated with a synthetic peptide of ˜35 aa residues. EBV glycoprotein-mediated membrane fusion with epithelial cells does not require gp42 but only gB and gH/gL. Recent observations indicate that EBV gH/gL engages integrins αvβ6 and/or αvβ8 on epithelial cells to trigger membrane fusion and entry. 
     The EBV gp350 glycoprotein encoded by BLLF1 is important for efficient EBV infection of resting B cells. Gp350 is the most abundant viral protein in the viral envelope. This large protein is heavily glycosylated and localizes to various subcellular compartments (cytoplasm, endoplasmic reticulum, Golgi, and plasma membrane) of replicating cells. EBV binds to primary B cells through its interaction with CD21, the complement receptor 2 (CR2) via gp350. Several gp350 domains appear to be involved in the formation of a stable complex with CD21, one of which has been identified as the receptor-binding site (amino acids [aa] 142 to 161). This glycan-free domain is also recognized by the neutralizing gp350-specific antibody 72A. 
     The present disclosure is not limited by a particular strain of EBV. The strain of EBV used in a vaccine may be any strain of EBV. 
     The present disclosure provides RNA (e.g., mRNA) vaccines against EBV infection—vaccines that elicit potent neutralizing antibodies and robust T cell responses against EBV antigens, inhibit the production of viral immunomodulatory factors, and/or prevent viral latency. 
     In some embodiments, vaccines disclosed herein are used therapeutically, i.e., following infection with EBV (to treat the infection). In some embodiments, the vaccines of the present disclosure can be used to prevent or reduce the frequency of Hodgkin&#39;s lymphoma, Burkitt&#39;s lymphoma, gastric carcinoma, nasopharyngeal carcinoma, post-transplant lymphoproliferative disease, diffuse B cell lymphoma, and/or NK/T cell lymphoma. 
     The EBV RNA vaccines described herein are superior to current vaccines in several ways. For example, the lipid nanoparticle (LNP) delivery system used herein increases the efficacy of RNA vaccines in comparison to other formulations, including a protamine-based approach described in the literature. The use of this LNP delivery system enables the effective delivery of chemically-modified RNA vaccines or unmodified RNA vaccines, without requiring additional adjuvant to produce a therapeutic result (e.g., production neutralizing antibody titer and/or a T cell response). In some embodiments, the EBV RNA vaccines disclosed herein are superior to conventional vaccines by a factor of at least 10 fold, 20, fold, 40, fold, 50 fold, 100 fold, 500 fold, or 1,000 fold when administered intramuscularly (IM) or intradermally (ID). These results can be achieved even when significantly lower doses of the RNA (e.g., mRNA) are administered in comparison with RNA doses used in other classes of lipid based formulations. 
     The LNP used in the studies described herein has been used previously to deliver siRNA in various animal models as well as in humans. In view of the observations made in association with the siRNA delivery of LNP formulations, the fact that LNP is useful in vaccines is quite surprising, particularly when immunity to an antigen has been hard to generate, as in the case of EBV. It has been observed that therapeutic delivery of siRNA formulated in LNP causes an undesirable inflammatory response associated with a transient IgM response, typically leading to a reduction in antigen production and a compromised immune response. In contrast to the findings observed with siRNA, the LNP-mRNA formulations of the present disclosure are demonstrated herein to generate enhanced IgG levels, sufficient for prophylactic and therapeutic methods rather than transient IgM responses. 
     Exemplary Epstein-Barr Virus (EBV) Antigens 
     Antigens are proteins capable of inducing an immune response (e.g., causing an immune system to produce antibodies against the antigens). Herein, use of the term antigen encompasses immunogenic proteins and immunogenic fragments (an immunogenic fragment that induces (or is capable of inducing) an immune response to EBV), unless otherwise stated. It should be understood that the term “protein’ encompasses peptides and the term “antigen” encompasses antigenic fragments. 
     A number of different antigens are associated with EBV. EBV vaccines, as provided herein, comprise at least one (one or more) ribonucleic acid (RNA, e.g., mRNA) having an open reading frame encoding at least one EBV antigen. Non-limiting examples of EBV antigens are provided below. 
     Exemplary EBV antigens are provided in the Sequence Listing elsewhere herein. For example, the antigens may be encoded by (thus the RNA may comprise or consist of) any one of sequences set forth in SEQ ID NO: 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, and/or 210. In some embodiments, the aforementioned sequences may further comprise a 5′ cap (e.g., 7mG(5′)ppp(5′)NlmpNp), a polyA tail, or a 5′ cap and a polyA tail. 
     It should be understood that the EBV vaccines of the present disclosure may comprise any of the RNA open reading frames (ORFs), or encode any of the protein ORFs, described herein, with or without a signal sequence. It should also be understood that the EBV vaccines of the present disclosure may include any 5′ untranslated region (UTR) and/or any 3′ UTR. Exemplary UTR sequences are provided in the Sequence Listing (e.g., SEQ ID NOs: 1, 3, 104 and 106; however, other UTR sequences (e.g., of the prior art) may be used or exchanged for any of the UTR sequences described herein. UTRs may also be omitted from the vaccine constructs provided herein. 
     EBV entry into B cells is initiated by attachment of glycoprotein gp350 to the complement receptor type 2 (CR2). A complex of three glycoproteins, gH, gL, and gp42, is subsequently required for penetration. gp42 binds to HLA class II, which functions as an entry mediator or co-receptor and, by analogy with other herpesviruses, gH is then thought to be involved virus-cell fusion. Entry of virus into epithelial cells is different. It can be initiated by attachment by an unknown glycoprotein in the absence of CR2. There is no interaction between gp42 and HLA class II and instead a distinct complex of only the two glycoproteins gH and gL interacts with a novel entry mediator. 
     EBV gH-gL complex includes of three glycoproteins, gp85, the gH homolog, which is the product of the BXLF2 open reading frame (ORF); gp25, the gL homolog, which is the product of the BKRF2 ORF; and gp42, which is the product of the BZLF2 ORF. The complex behaves in many respects like its counterparts in other herpesviruses. Glycoprotein gH is dependent on gL for authentic processing and transport, and the complex as a whole has been implicated as important to the ability of virus to fuse with the cell membrane and penetrate into the cytoplasm 
     The gp350 glycoprotein encoded by BLLF1 is important for efficient Epstein-Barr virus (EBV) infection of resting B cells. 
     The major EBV glycoprotein gp350 mediates docking of EBV on B cells by binding receptor type 2 (CR2) (Nemerow et al.,  J of Virol . (61):1416-1420 (1987); Szakonyi et al.,  Nat Struct Mol Biol . (13): 996-1001 (2006)). Due to alternative splicing, BLLF1 encodes gp350 and gp220, which are glycosylated and are approximately 350 and 220 kilodaltons in molecular weight, respectively (Beisel et al.,  J Virol . (54):665-674 (1985); Hummel et al.,  J Virol . (49):413-417 (1984)). In some embodiments, the EBV gp350 antigen comprises the sequence identified by SEQ ID NO: 81, 204, 185, 182, 207, or 208. 
     After EBV docking, EBV fuses with the plasma membrane of the host cell using a complex of glycoproteins. The core EBV membrane fusion machinery for entry into B cells and epithelial cells includes glycoprotein B (gB), glycoprotein H (gH) and glycoprotein L (gL) (Hutt-Fletcher et al.,  J Virol . (81): 7825-7832 (2007)). 
     gB is a single pass type 1 membrane protein also referred to as gp110 and is encoded by the BALF4 open reading frame (ORF) (Herrold et al.,  J of Virol . (70):2049-2054 (1996); Haan et al., Virology (290): 106-114 (2001)); McShane et al.,  Proc Natl Acad Sci USA . (101): 17474-17479 (2004)). In some embodiments, the EBV gB antigen comprises the sequence identified by SEQ ID NO: 209. 
     gH (also referred to as gp85) is a type 1 transmembrane protein encoded by the open reading frame (ORF) of the BXLF2 gene (Heineman et al.,  J Virol . (62):1101-1107 (1988)); Oba et al.,  J Virol . (62):1108-1114 (1988)). In some embodiments, the EBV gH antigen comprises the sequence identified by SEQ ID NO: 187. 
     gL (also referred to as gp25) and encoded by BKRF2 ORF is required for proper folding and localization of gH (Li et al.,  J Virol . (69): 3987-3994 (1995); Yaswen et al.,  Virology.  (195): 387-396 (1993)). Therefore, gH and gL often functions as a complex to mediate viral fusion and this complex has been crystallized (Matsurra et al.,  Proc Natl Acad Sci USA . (107):22641-2264 (2010)). In some embodiments, the EBV gL antigen comprises the sequence identified by SEQ ID NO: 188. 
     In addition to the core membrane fusion machinery, EBV entry into B cells requires gp42, which is encoded by a BZLF2 ORF (Kirschner et al.,  J. Virol . (80):9444-54 (2006); Wang et al.,  J. Virol ., (72):5552-5558 (1998); Silva et al.,  J. Virol . (78): 5946-5956 (2004); Li et al.  J. Virol ., (69):3987-3994 (1995). EBV gp42 mediates viral fusion with B cells by binding MHC class II molecules (Mullen et al.,  Molecular Cell . (9):375-385 (2002); Haan et al.  J Virol . (74): 2451-4 (2000)). In some embodiments, the EBV gp42 antigen comprises the sequence identified by SEQ ID NO: 189. 
     Latent membrane protein 1 (LMP1) is a six transmembrane domain protein that promotes immortalization of resting B cells and helps protect EBV-infected B cells from apoptosis (Hennessy et al.,  Proc Natl. Acad. Sci USA . (81): 7207-11 (1984); Kaye et al.,  Proc Natl Acad Sci USA . (90): 9150-9154 (1993); Henderson et al.,  Cell  (65): 1107-1115 (1991)). A number of signaling pathways may be activated by LMP1, including tumor necrosis factor receptor family signaling and DNA synthesis (Peng et al.  Oncogene . (7): 1775-1782; Masialos et al.,  Cell . (80):389-399 (1995); Li et al.,  J Biomed Sci . (10):490-504 (2003)). Furthermore, LMP1 signaling can upregulate expression of the anti-apoptosis Bc1-2 oncogene in B cells (Rowe et al.,  J Virol . (68):5602-12 (1994)). In some embodiments, the EBV LMP1 antigen comprises the sequence identified by SEQ ID NO: 179. 
     Similar to LMP1, Latent membrane protein 2 (LMP2) is an EBV-encoded transmembrane protein that is often expressed in latently infected cells. There are two isoforms of LMP2 (LMP2A and LMP2B) (Laux et al.,  EMBO J . (7):769-74 (1988); Longnecker et al.,  J Virol . (64):2319-26 (1990)). LMP2A is implicated in maintaining EBV latency. For example, LMP2A can exclude B-cell receptor (BCR) from lipid rafts to prevent lytic induction (Dykstra et al.,  Immunity . (14):57-67 (2001)). LMP2A can also activate the phosphatidylinositol 3-kinase (PI3-K)/Akt pathway to promote cell survival (Scholle et al.,  J Virol . (74): 10681-10689 (2000); Swart et al.  J Virol . (74): 10838-10845 (2000); Fukuda et al.,  J. Virol . (78): 1697-16705 (2004)). LMP2B protein generally lacks 119 amino-terminal amino acids compared to LMP2A and is implicated in epithelial cell spreading and motility (Allen et al.,  J Virol . (79):1789-1802 (2005)). In some embodiments, the EBV LMP2 antigen comprises the sequence identified by SEQ ID NO: 181. 
     Epstein-Barr nuclear antigens (ENBAs) that help establish latent infection include EBNA1, EBNA2, EBNA3A and EBNA3C. EBNA1 encoded by BKRF1 promotes viral DNA replication, episomal maintenance and episomal partitioning (Rawlins et al.,  Cell  (42): 859-68 (1985); (Hung et al.,  Proc Natl Acad  Sciences USA (98): 1865-1870 (2001)). In particular, EBNA1 can bind family of repeats and dyad symmetry elements of the latent origin oriP. In some embodiments, the EBV EBNA1 antigen comprises the sequence identified by SEQ ID NO: 178. 
     There are three members of the EBNA3 family: EBNA3A, EBNA3B and EBNA3C. EBNA3s regulate transcription by binding RBPJ, which is a transcriptional regulator in the Notch signaling pathway (Zhao et al.,  J Virol . (70):4228-4236 (1996); Robertson et al.,  J Virol . (69):3108-3116 (1995); Robertson et al.  J Virol . (70):3068-3074 (1996)). In particular, EBNA3A and EBNA3C have been shown to be required for EBV-mediated transformation of B cells (Tomkinson et al.  J Virol . (67):2014-25 (1993)). In some embodiments, the EBV EBNA3A antigen comprises the sequence identified by SEQ ID NO: 177. 
     Nucleic Acids 
     The EBV vaccines of the present disclosure comprise at least one (one or more) ribonucleic acid (RNA) having an open reading frame encoding at least one EBV antigen. In some embodiments, the RNA is a messenger RNA (mRNA) having an open reading frame encoding at least one EBV antigen. In some embodiments, the RNA (e.g., mRNA) further comprises a (at least one) 5′ UTR, 3′ UTR, a polyA tail and/or a 5′ cap. 
     Nucleic acids comprise a polymer of nucleotides (nucleotide monomers), also referred to as polynucleotides. Nucleic acids may be or may include, for example, deoxyribonucleic acids (DNAs), ribonucleic acids (RNAs), threose nucleic acids (TNAs), glycol nucleic acids (GNAs), peptide nucleic acids (PNAs), locked nucleic acids (LNAs, including LNA having a β-D-ribo configuration, α-LNA having an α-L-ribo configuration (a diastereomer of LNA), 2′-amino-LNA having a 2′-amino functionalization, and 2′-amino-α-LNA having a 2′-amino functionalization), ethylene nucleic acids (ENA), cyclohexenyl nucleic acids (CeNA) and/or chimeras and/or combinations thereof. 
     Messenger RNA (mRNA) is any ribonucleic acid that encodes a (at least one) protein (a naturally-occurring, non-naturally-occurring, or modified polymer of amino acids) and can be translated to produce the encoded protein in vitro, in vivo, in situ or ex vivo. The skilled artisan will appreciate that, except where otherwise noted, nucleic acid sequences set forth in the instant application may recite “T”s in a representative DNA sequence but where the sequence represents RNA (e.g., mRNA), the “T”s would be substituted for “U”s. Thus, any of the DNAs disclosed and identified by a particular sequence identification number herein also disclose the corresponding RNA (e.g., mRNA) sequence complementary to the DNA, where each “T” of the DNA sequence is substituted with “U.” 
     An open reading frame (ORF) is a continuous stretch of DNA or RNA beginning with a start codon (e.g., methionine (ATG or AUG)) and ending with a stop codon (e.g., TAA, TAG or TGA, or UAA, UAG or UGA). An ORF typically encodes a protein. It will be understood that the sequences disclosed herein may further comprise additional elements, e.g., 5′ and 3′ UTRs, but that those elements, unlike the ORF, need not necessarily be present in a vaccine of the present disclosure. 
     Variants 
     In some embodiments, an RNA of the present disclosure encodes an EBV antigen variant. Antigen or other polypeptide variants refers to molecules that differ in their amino acid sequence from a wild-type, native or reference sequence. The antigen/polypeptide variants may possess substitutions, deletions, and/or insertions at certain positions within the amino acid sequence, as compared to a native or reference sequence. Ordinarily, variants possess at least 50% identity to a wild-type, native or reference sequence. In some embodiments, variants share at least 80%, or at least 90% identity with a wild-type, native or reference sequence. 
     Variant antigens/polypeptides encoded by nucleic acids of the disclosure may contain amino acid changes that confer any of a number of desirable properties, e.g., that enhance their immunogenicity, enhance their expression, and/or improve their stability or PK/PD properties in a subject. Variant antigens/polypeptides can be made using routine mutagenesis techniques and assayed as appropriate to determine whether they possess the desired property. Assays to determine expression levels and immunogenicity are well known in the art and exemplary such assays are set forth in the Examples section. Similarly, PK/PD properties of a protein variant can be measured using art recognized techniques, e.g., by determining expression of antigens in a vaccinated subject over time and/or by looking at the durability of the induced immune response. The stability of protein(s) encoded by a variant nucleic acid may be measured by assaying thermal stability or stability upon urea denaturation or may be measured using in silico prediction. Methods for such experiments and in silico determinations are known in the art. 
     In some embodiments, an EBV vaccine comprises an mRNA ORF having a nucleotide sequence identified by any one of the sequences provided herein (see e.g., Sequence Listing), or having a nucleotide sequence at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identical to a nucleotide sequence identified by any one of the sequence provided herein. 
     The term “identity” refers to a relationship between the sequences of two or more polypeptides (e.g. antigens) or polynucleotides (nucleic acids), as determined by comparing the sequences. Identity also refers to the degree of sequence relatedness between or among sequences as determined by the number of matches between strings of two or more amino acid residues or nucleic acid residues. Identity measures the percent of identical matches between the smaller of two or more sequences with gap alignments (if any) addressed by a particular mathematical model or computer program (e.g., “algorithms”). Identity of related antigens or nucleic acids can be readily calculated by known methods. “Percent (%) identity” as it applies to polypeptide or polynucleotide sequences is defined as the percentage of residues (amino acid residues or nucleic acid residues) in the candidate amino acid or nucleic acid sequence that are identical with the residues in the amino acid sequence or nucleic acid sequence of a second sequence after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent identity. Methods and computer programs for the alignment are well known in the art. It is understood that identity depends on a calculation of percent identity but may differ in value due to gaps and penalties introduced in the calculation. Generally, variants of a particular polynucleotide or polypeptide (e.g., antigen) have at least 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% but less than 100% sequence identity to that particular reference polynucleotide or polypeptide as determined by sequence alignment programs and parameters described herein and known to those skilled in the art. Such tools for alignment include those of the BLAST suite (Stephen F. Altschul, et al (1997), “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs”,  Nucleic Acids Res.  25:3389-3402). Another popular local alignment technique is based on the Smith-Waterman algorithm (Smith, T. F. &amp; Waterman, M. S. (1981) “Identification of common molecular subsequences.”  J. Mol. Biol.  147:195-197). A general global alignment technique based on dynamic programming is the Needleman-Wunsch algorithm (Needleman, S. B. &amp; Wunsch, C. D. (1970) “A general method applicable to the search for similarities in the amino acid sequences of two proteins.”  J. Mol. Biol.  48:443-453). More recently a Fast Optimal Global Sequence Alignment Algorithm (FOGSAA) has been developed that purportedly produces global alignment of nucleotide and protein sequences faster than other optimal global alignment methods, including the Needleman-Wunsch algorithm. 
     As such, polynucleotides encoding peptides or polypeptides containing substitutions, insertions and/or additions, deletions and covalent modifications with respect to reference sequences, in particular the polypeptide (e.g., antigen) sequences disclosed herein, are included within the scope of this disclosure. For example, sequence tags or amino acids, such as one or more lysines, can be added to peptide sequences (e.g., at the N-terminal or C-terminal ends). Sequence tags can be used for peptide detection, purification or localization. Lysines can be used to increase peptide solubility or to allow for biotinylation. Alternatively, amino acid residues located at the carboxy and amino terminal regions of the amino acid sequence of a peptide or protein may optionally be deleted providing for truncated sequences. Certain amino acids (e.g., C-terminal or N-terminal residues) may alternatively be deleted depending on the use of the sequence, as for example, expression of the sequence as part of a larger sequence which is soluble, or linked to a solid support. In some embodiments, sequences for (or encoding) signal sequences, termination sequences, transmembrane domains, linkers, multimerization domains (such as, e.g., foldon regions) and the like may be substituted with alternative sequences that achieve the same or a similar function. In some embodiments, cavities in the core of proteins can be filled to improve stability, e.g., by introducing larger amino acids. In other embodiments, buried hydrogen bond networks may be replaced with hydrophobic resides to improve stability. In yet other embodiments, glycosylation sites may be removed and replaced with appropriate residues. Such sequences are readily identifiable to one of skill in the art. It should also be understood that some of the sequences provided herein contain sequence tags or terminal peptide sequences (e.g., at the N-terminal or C-terminal ends) that may be deleted, for example, prior to use in the preparation of an RNA (e.g., mRNA) vaccine. 
     As recognized by those skilled in the art, protein fragments, functional protein domains, and homologous proteins are also considered to be within the scope of EBV antigens of interest. For example, provided herein is any protein fragment (meaning a polypeptide sequence at least one amino acid residue shorter than a reference antigen sequence but otherwise identical) of a reference protein, provided that the fragment is immunogenic and confers a protective immune response to the EBV pathogen. In addition to variants that are identical to the reference protein but are truncated, in some embodiments, an antigen includes 2, 3, 4, 5, 6, 7, 8, 9, 10, or more mutations, as shown in any of the sequences provided or referenced herein. Antigens/antigenic polypeptides can range in length from about 4, 6, or 8 amino acids to full length proteins. 
     Stabilizing Elements 
     Naturally-occurring eukaryotic mRNA molecules can contain stabilizing elements, including, but not limited to untranslated regions (UTR) at their 5′-end (5′ UTR) and/or at their 3′-end (3′ UTR), in addition to other structural features, such as a 5′-cap structure or a 3′-poly(A) tail. Both the 5′ UTR and the 3′ UTR are typically transcribed from the genomic DNA and are elements of the premature mRNA. Characteristic structural features of mature mRNA, such as the 5′-cap and the 3′-poly(A) tail are usually added to the transcribed (premature) mRNA during mRNA processing. 
     In some embodiments, a vaccine includes at least one RNA polynucleotide having an open reading frame encoding at least one antigenic polypeptide having at least one modification, at least one 5′ terminal cap, and is formulated within a lipid nanoparticle. 5′-capping of polynucleotides may be completed concomitantly during the in vitro-transcription reaction using the following chemical RNA cap analogs to generate the 5′-guanosine cap structure according to manufacturer protocols: 3′-O-Me-m7G(5′)ppp(5′) G [the ARCA cap]; G(5′)ppp(5′)A; G(5′)ppp(5′)G; m7G(5′)ppp(5′)A; m7G(5′)ppp(5′)G (New England BioLabs, Ipswich, Mass.). 5′-capping of modified RNA may be completed post-transcriptionally using a Vaccinia Virus Capping Enzyme to generate the “Cap 0” structure: m7G(5′)ppp(5′)G (New England BioLabs, Ipswich, Mass.). Cap 1 structure may be generated using both Vaccinia Virus Capping Enzyme and a 2′-O methyl-transferase to generate: m7G(5′)ppp(5′)G-2′-O-methyl. Cap 2 structure may be generated from the Cap 1 structure followed by the 2′-O-methylation of the 5′-antepenultimate nucleotide using a 2′-O methyl-transferase. Cap 3 structure may be generated from the Cap 2 structure followed by the 2′-O-methylation of the 5′-preantepenultimate nucleotide using a 2′-O methyl-transferase. Enzymes may be derived from a recombinant source. 
     The 3′-poly(A) tail is typically a stretch of adenine nucleotides added to the 3′-end of the transcribed mRNA. It can, in some instances, comprise up to about 400 adenine nucleotides. In some embodiments, the length of the 3′-poly(A) tail may be an essential element with respect to the stability of the individual mRNA. 
     In some embodiments, EBV RNA vaccines may include one or more stabilizing elements. Stabilizing elements may include for instance a histone stem-loop. A stem-loop binding protein (SLBP), a 32 kDa protein has been identified. It is associated with the histone stem-loop at the 3′-end of the histone messages in both the nucleus and the cytoplasm. Its expression level is regulated by the cell cycle; it peaks during the S-phase, when histone mRNA levels are also elevated. The protein has been shown to be essential for efficient 3′-end processing of histone pre-mRNA by the U7 snRNP. SLBP continues to be associated with the stem-loop after processing, and then stimulates the translation of mature histone mRNAs into histone proteins in the cytoplasm. The RNA binding domain of SLBP is conserved through metazoa and protozoa; its binding to the histone stem-loop depends on the structure of the loop. The minimum binding site includes at least three nucleotides 5′ and two nucleotides 3′ relative to the stem-loop. 
     In some embodiments, EBV RNA vaccines include a coding region, at least one histone stem-loop, and optionally, a poly(A) sequence or polyadenylation signal. The poly(A) sequence or polyadenylation signal generally should enhance the expression level of the encoded protein. 
     The encoded protein, in some embodiments, is not a histone protein, a reporter protein (e.g. Luciferase, GFP, EGFP, β-Galactosidase, EGFP), or a marker or selection protein (e.g. alpha-Globin, Galactokinase and Xanthine:guanine phosphoribosyl transferase (GPT)). 
     In some embodiments, the combination of a poly(A) sequence or polyadenylation signal and at least one histone stem-loop, even though both represent alternative mechanisms in nature, acts synergistically to increase the protein expression beyond the level observed with either of the individual elements. The synergistic effect of the combination of poly(A) and at least one histone stem-loop does not depend on the order of the elements or the length of the poly(A) sequence. 
     In some embodiments, EBV RNA vaccines do not comprise a histone downstream element (HDE). “Histone downstream element” (HDE) includes a purine-rich polynucleotide stretch of approximately 15 to 20 nucleotides 3′ of naturally occurring stem-loops, representing the binding site for the U7 snRNA, which is involved in processing of histone pre-mRNA into mature histone mRNA. In some embodiments, the nucleic acid does not include an intron. 
     In some embodiments, EBV RNA vaccines may or may not contain an enhancer and/or promoter sequence, which may be modified or unmodified or which may be activated or inactivated. In some embodiments, the histone stem-loop is generally derived from histone genes, and includes an intramolecular base pairing of two neighbored partially or entirely reverse complementary sequences separated by a spacer, consisting of a short sequence, which forms the loop of the structure. The unpaired loop region is typically unable to base pair with either of the stem loop elements. It occurs more often in RNA, as is a key component of many RNA secondary structures, but may be present in single-stranded DNA as well. Stability of the stem-loop structure generally depends on the length, number of mismatches or bulges, and base composition of the paired region. In some embodiments, wobble base pairing (non-Watson-Crick base pairing) may result. In some embodiments, the at least one histone stem-loop sequence comprises a length of 15 to 45 nucleotides. 
     In some embodiments, EBV RNA vaccines may have one or more AU-rich sequences removed. These sequences, sometimes referred to as AURES are destabilizing sequences found in the 3′UTR. The AURES may be removed from the RNA vaccines. Alternatively the AURES may remain in the RNA vaccine. 
     Signal Peptides 
     In some embodiments, an EBV vaccine comprises a RNA having an ORF that encodes a signal peptide fused to the EBV antigen. Signal peptides, comprising the N-terminal 15-60 amino acids of proteins, are typically needed for the translocation across the membrane on the secretory pathway and, thus, universally control the entry of most proteins both in eukaryotes and prokaryotes to the secretory pathway. In eukaryotes, the signal peptide of a nascent precursor protein (pre-protein) directs the ribosome to the rough endoplasmic reticulum (ER) membrane and initiates the transport of the growing peptide chain across it for processing. ER processing produces mature proteins, wherein the signal peptide is cleaved from precursor proteins, typically by a ER-resident signal peptidase of the host cell, or they remain uncleaved and function as a membrane anchor. A signal peptide may also facilitate the targeting of the protein to the cell membrane. 
     A signal peptide may have a length of 15-60 amino acids. For example, a signal peptide may have a length of 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 amino acids. In some embodiments, a signal peptide has a length of 20-60, 25-60, 30-60, 35-60, 40-60, 45-60, 50-60, 55-60, 15-55, 20-55, 25-55, 30-55, 35-55, 40-55, 45-55, 50-55, 15-50, 20-50, 25-50, 30-50, 35-50, 40-50, 45-50, 15-45, 20-45, 25-45, 30-45, 35-45, 40-45, 15-40, 20-40, 25-40, 30-40, 35-40, 15-35, 20-35, 25-35, 30-35, 15-30, 20-30, 25-30, 15-25, 20-25, or 15-20 amino acids. 
     Signal peptides from heterologous genes (which regulate expression of genes other than EBV antigens in nature) are known in the art and can be tested for desired properties and then incorporated into a nucleic acid of the disclosure. In some embodiments, the signal peptide is a bovine prolactin signal peptide. For example, the bovine prolactin signal peptide may comprise sequence MDSKGSSQKGSRLLLLLVVSNLLLPQGVVG (SEQ ID NO: 115). Other signal peptide sequences may also be used. For example, the signal peptide may comprise one of the following sequences: MDWTWILFLVAAATRVHS (SEQ ID NO: 116); METPAQLLFLLLLWLPDTTG (SEQ ID NO: 117); MLGSNSGQRVVFTILLLLVAPAYS (SEQ ID NO: 118); MKCLLYLAFLFIGVNCA (SEQ ID NO: 119); MWLVSLAIVTACAGA (SEQ ID NO: 120). 
     Fusion Proteins 
     In some embodiments, an EBV RNA vaccine of the present disclosure includes an RNA encoding an antigenic fusion protein. Thus, the encoded antigen or antigens may include two or more proteins (e.g., protein and/or protein fragment) joined together. Alternatively, the protein to which a protein antigen is fused does not promote a strong immune response to itself, but rather to the EBV antigen. Antigenic fusion proteins, in some embodiments, retain the functional property from each original protein. 
     Scaffold Moieties 
     The RNA (e.g., mRNA) vaccines as provided herein, in some embodiments, encode fusion proteins which comprise EBV antigens linked to scaffold moieties. In some embodiments, such scaffold moieties impart desired properties to an antigen encoded by a nucleic acid of the disclosure. For example scaffold proteins may improve the immunogenicity of an antigen, e.g., by altering the structure of the antigen, altering the uptake and processing of the antigen, and/or causing the antigen to bind to a binding partner. 
     In some embodiments, the scaffold moiety is protein that can self-assemble into protein nanoparticles that are highly symmetric, stable, and structurally organized, with diameters of 10-150 nm, a highly suitable size range for optimal interactions with various cells of the immune system. In some embodiments, viral proteins or virus-like particles can be used to form stable nanoparticle structures. Examples of such viral proteins are known in the art. For example, in some embodiments, the scaffold moiety is a hepatitis B surface antigen (HBsAg). HBsAg forms spherical particles with an average diameter of ˜22 nm and which lacked nucleic acid and hence are non-infectious (Lopez-Sagaseta, J. et al.  Computational and Structural Biotechnology Journal  14 (2016) 58-68). In some embodiments, the scaffold moiety is a hepatitis B core antigen (HBcAg) self-assembles into particles of 24-31 nm diameter, which resembled the viral cores obtained from HBV-infected human liver. HBcAg produced in self-assembles into two classes of differently sized nanoparticles of 300 Å and 360 Å diameter, corresponding to 180 or 240 protomers. In some embodiments an EBV antigen is fused to HBsAG or HBcAG to facilitate self-assembly of nanoparticles displaying the EBV antigen. 
     In another embodiment, bacterial protein platforms may be used. Non-limiting examples of these self-assembling proteins include ferritin, lumazine and encapsulin. 
     Ferritin is a protein whose main function is intracellular iron storage. Ferritin is made of 24 subunits, each composed of a four-alpha-helix bundle, that self-assemble in a quaternary structure with octahedral symmetry (Cho K. J. et al.  J Mol Biol.  2009; 390:83-98). Several high-resolution structures of ferritin have been determined, confirming that  Helicobacter pylori  ferritin is made of 24 identical protomers, whereas in animals, there are ferritin light and heavy chains that can assemble alone or combine with different ratios into particles of 24 subunits (Granier T. et al.  J Biol Inorg Chem.  2003; 8:105-111; Lawson D. M. et al. Nature. 1991; 349:541-544). Ferritin self-assembles into nanoparticles with robust thermal and chemical stability. Thus, the ferritin nanoparticle is well-suited to carry and expose antigens. 
     Lumazine synthase (LS) is also well-suited as a nanoparticle platform for antigen display. LS, which is responsible for the penultimate catalytic step in the biosynthesis of riboflavin, is an enzyme present in a broad variety of organisms, including archaea, bacteria, fungi, plants, and eubacteria (Weber S. E.  Flavins and Flavoproteins . Methods and Protocols, Series: Methods in Molecular Biology. 2014). The LS monomer is 150 amino acids long, and consists of beta-sheets along with tandem alpha-helices flanking its sides. A number of different quaternary structures have been reported for LS, illustrating its morphological versatility: from homopentamers up to symmetrical assemblies of 12 pentamers forming capsids of 150 Å diameter. Even LS cages of more than 100 subunits have been described (Zhang X. et al.  J Mol Biol.  2006; 362:753-770). 
     Encapsulin, a novel protein cage nanoparticle isolated from thermophile  Thermotoga maritima , may also be used as a platform to present antigens on the surface of self-assembling nanoparticles. Encapsulin is assembled from 60 copies of identical 31 kDa monomers having a thin and icosahedral T=1 symmetric cage structure with interior and exterior diameters of 20 and 24 nm, respectively (Sutter M. et al.  Nat Struct Mol Biol.  2008, 15: 939-947). Although the exact function of encapsulin in  T. maritima  is not clearly understood yet, its crystal structure has been recently solved and its function was postulated as a cellular compartment that encapsulates proteins such as DyP (Dye decolorizing peroxidase) and Flp (Ferritin like protein), which are involved in oxidative stress responses (Rahmanpour R. et al.  FEBS J.  2013, 280: 2097-2104). 
     Linkers and Cleavable Peptides 
     In some embodiments, the mRNAs of the disclosure encode more than one polypeptide, referred to herein as fusion proteins. In some embodiments, the mRNA further encodes a linker located between at least one or each domain of the fusion protein. The linker can be, for example, a cleavable linker or protease-sensitive linker. In some embodiments, the linker is selected from the group consisting of F2A linker, P2A linker, T2A linker, E2A linker, and combinations thereof. This family of self-cleaving peptide linkers, referred to as 2A peptides, has been described in the art (see for example, Kim, J. H. et al. (2011)  PLoS ONE  6:e18556). In some embodiments, the linker is an F2A linker. In some embodiments, the linker is a GGGS linker or a GGGGS linker, for example, including one or more (e.g., 1, 2, 3, 4, or more) repeat GGGS (SEQ ID NO: 226) or GGGGS (SEQ ID NO: 227) sequences (e.g., GGGGS GGGGS GGGGS (SEQ ID NO: 224) and/or GGGGS GGGGS GGGGS GGGGS (SEQ ID NO: 225)). In some embodiments, the fusion protein contains three domains with intervening linkers, having the structure: domain-linker-domain-linker-domain. 
     Cleavable linkers known in the art may be used in connection with the disclosure. Exemplary such linkers include: F2A linkers, T2A linkers, P2A linkers, E2A linkers (See, e.g., WO2017/127750). The skilled artisan will appreciate that other art-recognized linkers may be suitable for use in the constructs of the disclosure (e.g., encoded by the nucleic acids of the disclosure). The skilled artisan will likewise appreciate that other polycistronic constructs (mRNA encoding more than one antigen/polypeptide separately within the same molecule) may be suitable for use as provided herein. 
     Sequence Optimization 
     In some embodiments, an ORF encoding an antigen of the disclosure is codon optimized. Codon optimization methods are known in the art. For example, an ORF of any one or more of the sequences provided herein may be codon optimized. Codon optimization, in some embodiments, may be used to match codon frequencies in target and host organisms to ensure proper folding; bias GC content to increase mRNA stability or reduce secondary structures; minimize tandem repeat codons or base runs that may impair gene construction or expression; customize transcriptional and translational control regions; insert or remove protein trafficking sequences; remove/add post translation modification sites in encoded protein (e.g., glycosylation sites); add, remove or shuffle protein domains; insert or delete restriction sites; modify ribosome binding sites and mRNA degradation sites; adjust translational rates to allow the various domains of the protein to fold properly; or reduce or eliminate problem secondary structures within the polynucleotide. Codon optimization tools, algorithms and services are known in the art—non-limiting examples include services from GeneArt (Life Technologies), DNA2.0 (Menlo Park Calif.) and/or proprietary methods. In some embodiments, the open reading frame (ORF) sequence is optimized using optimization algorithms. 
     In some embodiments, a codon optimized sequence shares less than 95% sequence identity to a naturally-occurring or wild-type sequence ORF (e.g., a naturally-occurring or wild-type mRNA sequence encoding an EBV antigen). In some embodiments, a codon optimized sequence shares less than 90% sequence identity to a naturally-occurring or wild-type sequence (e.g., a naturally-occurring or wild-type mRNA sequence encoding an EBV antigen). In some embodiments, a codon optimized sequence shares less than 85% sequence identity to a naturally-occurring or wild-type sequence (e.g., a naturally-occurring or wild-type mRNA sequence encoding an EBV antigen). In some embodiments, a codon optimized sequence shares less than 80% sequence identity to a naturally-occurring or wild-type sequence (e.g., a naturally-occurring or wild-type mRNA sequence encoding an EBV antigen). In some embodiments, a codon optimized sequence shares less than 75% sequence identity to a naturally-occurring or wild-type sequence (e.g., a naturally-occurring or wild-type mRNA sequence encoding an EBV antigen). 
     In some embodiments, a codon optimized sequence shares between 65% and 85% (e.g., between about 67% and about 85% or between about 67% and about 80%) sequence identity to a naturally-occurring or wild-type sequence (e.g., a naturally-occurring or wild-type mRNA sequence encoding an EBV antigen). In some embodiments, a codon optimized sequence shares between 65% and 75% or about 80% sequence identity to a naturally-occurring or wild-type sequence (e.g., a naturally-occurring or wild-type mRNA sequence encoding an EBV antigen). 
     In some embodiments, a codon-optimized sequence encodes an antigen that is as immunogenic as, or more immunogenic than (e.g., at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 100%, or at least 200% more), than an EBV antigen encoded by a non-codon-optimized sequence. 
     When transfected into mammalian host cells, the modified mRNAs have a stability of between 12-18 hours, or greater than 18 hours, e.g., 24, 36, 48, 60, 72, or greater than 72 hours and are capable of being expressed by the mammalian host cells. 
     In some embodiments, a codon optimized RNA may be one in which the levels of G/C are enhanced. The G/C-content of nucleic acid molecules (e.g., mRNA) may influence the stability of the RNA. RNA having an increased amount of guanine (G) and/or cytosine (C) residues may be functionally more stable than RNA containing a large amount of adenine (A) and thymine (T) or uracil (U) nucleotides. As an example, WO02/098443 discloses a pharmaceutical composition containing an mRNA stabilized by sequence modifications in the translated region. Due to the degeneracy of the genetic code, the modifications work by substituting existing codons for those that promote greater RNA stability without changing the resulting amino acid. The approach is limited to coding regions of the RNA. 
     Chemically Unmodified Nucleotides 
     In some embodiments, at least one RNA (e.g., mRNA) of an EBV vaccines of the present disclosure is not chemically modified and comprises the standard ribonucleotides consisting of adenosine, guanosine, cytosine and uridine. In some embodiments, nucleotides and nucleosides of the present disclosure comprise standard nucleoside residues such as those present in transcribed RNA (e.g. A, G, C, or U). In some embodiments, nucleotides and nucleosides of the present disclosure comprise standard deoxyribonucleosides such as those present in DNA (e.g. dA, dG, dC, or dT). 
     Chemical Modifications 
     EBV RNA vaccines of the present disclosure comprise, in some embodiments, at least one nucleic acid (e.g., RNA) having an open reading frame encoding at least one EBV antigen, wherein the nucleic acid comprises nucleotides and/or nucleosides that can be standard (unmodified) or modified as is known in the art. In some embodiments, nucleotides and nucleosides of the present disclosure comprise modified nucleotides or nucleosides. Such modified nucleotides and nucleosides can be naturally-occurring modified nucleotides and nucleosides or non-naturally occurring modified nucleotides and nucleosides. Such modifications can include those at the sugar, backbone, or nucleobase portion of the nucleotide and/or nucleoside as are recognized in the art. 
     In some embodiments, a naturally-occurring modified nucleotide or nucleotide of the disclosure is one as is generally known or recognized in the art. Non-limiting examples of such naturally occurring modified nucleotides and nucleotides can be found, inter alia, in the widely recognized MODOMICS database. 
     In some embodiments, a non-naturally occurring modified nucleotide or nucleoside of the disclosure is one as is generally known or recognized in the art. Non-limiting examples of such non-naturally occurring modified nucleotides and nucleosides can be found, inter alia, in published US application Nos. PCT/US2012/058519; PCT/US2013/075177; PCT/US2014/058897; PCT/US2014/058891; PCT/US2014/070413; PCT/US2015/36773; PCT/US2015/36759; PCT/US2015/36771; or PCT/IB2017/051367 all of which are incorporated by reference herein. 
     Hence, nucleic acids of the disclosure (e.g., DNA nucleic acids and RNA nucleic acids, such as mRNA nucleic acids) can comprise standard nucleotides and nucleosides, naturally-occurring nucleotides and nucleosides, non-naturally-occurring nucleotides and nucleosides, or any combination thereof. 
     Nucleic acids of the disclosure (e.g., DNA nucleic acids and RNA nucleic acids, such as mRNA nucleic acids), in some embodiments, comprise various (more than one) different types of standard and/or modified nucleotides and nucleosides. In some embodiments, a particular region of a nucleic acid contains one, two or more (optionally different) types of standard and/or modified nucleotides and nucleosides. 
     In some embodiments, a modified RNA nucleic acid (e.g., a modified mRNA nucleic acid), introduced to a cell or organism, exhibits reduced degradation in the cell or organism, respectively, relative to an unmodified nucleic acid comprising standard nucleotides and nucleosides. 
     In some embodiments, a modified RNA nucleic acid (e.g., a modified mRNA nucleic acid), introduced into a cell or organism, may exhibit reduced immunogenicity in the cell or organism, respectively (e.g., a reduced innate response) relative to an unmodified nucleic acid comprising standard nucleotides and nucleosides. 
     Nucleic acids (e.g., RNA nucleic acids, such as mRNA nucleic acids), in some embodiments, comprise non-natural modified nucleotides that are introduced during synthesis or post-synthesis of the nucleic acids to achieve desired functions or properties. The modifications may be present on internucleotide linkages, purine or pyrimidine bases, or sugars. The modification may be introduced with chemical synthesis or with a polymerase enzyme at the terminal of a chain or anywhere else in the chain. Any of the regions of a nucleic acid may be chemically modified. 
     The present disclosure provides for modified nucleosides and nucleotides of a nucleic acid (e.g., RNA nucleic acids, such as mRNA nucleic acids). A “nucleoside” refers to a compound containing a sugar molecule (e.g., a pentose or ribose) or a derivative thereof in combination with an organic base (e.g., a purine or pyrimidine) or a derivative thereof (also referred to herein as “nucleobase”). A “nucleotide” refers to a nucleoside, including a phosphate group. Modified nucleotides may by synthesized by any useful method, such as, for example, chemically, enzymatically, or recombinantly, to include one or more modified or non-natural nucleosides. Nucleic acids can comprise a region or regions of linked nucleosides. Such regions may have variable backbone linkages. The linkages can be standard phosphodiester linkages, in which case the nucleic acids would comprise regions of nucleotides. 
     Modified nucleotide base pairing encompasses not only the standard adenosine-thymine, adenosine-uracil, or guanosine-cytosine base pairs, but also base pairs formed between nucleotides and/or modified nucleotides comprising non-standard or modified bases, wherein the arrangement of hydrogen bond donors and hydrogen bond acceptors permits hydrogen bonding between a non-standard base and a standard base or between two complementary non-standard base structures, such as, for example, in those nucleic acids having at least one chemical modification. One example of such non-standard base pairing is the base pairing between the modified nucleotide inosine and adenine, cytosine or uracil. Any combination of base/sugar or linker may be incorporated into nucleic acids of the present disclosure. 
     In some embodiments, modified nucleobases in nucleic acids (e.g., RNA nucleic acids, such as mRNA nucleic acids) comprise 1-methyl-pseudouridine (m1ψ), 1-ethyl-pseudouridine (e1ψ), 5-methoxy-uridine (mo5U), 5-methyl-cytidine (m5C), and/or pseudouridine (ψ). In some embodiments, modified nucleobases in nucleic acids (e.g., RNA nucleic acids, such as mRNA nucleic acids) comprise 5-methoxymethyl uridine, 5-methylthio uridine, 1-methoxymethyl pseudouridine, 5-methyl cytidine, and/or 5-methoxy cytidine. In some embodiments, the polyribonucleotide includes a combination of at least two (e.g., 2, 3, 4 or more) of any of the aforementioned modified nucleobases, including but not limited to chemical modifications. 
     In some embodiments, a RNA nucleic acid of the disclosure comprises 1-methyl-pseudouridine (m1ψ) substitutions at one or more or all uridine positions of the nucleic acid. 
     In some embodiments, a RNA nucleic acid of the disclosure comprises 1-methyl-pseudouridine (m1ψ) substitutions at one or more or all uridine positions of the nucleic acid and 5-methyl cytidine substitutions at one or more or all cytidine positions of the nucleic acid. 
     In some embodiments, a RNA nucleic acid of the disclosure comprises pseudouridine (ψ) substitutions at one or more or all uridine positions of the nucleic acid. 
     In some embodiments, a RNA nucleic acid of the disclosure comprises pseudouridine (ψ) substitutions at one or more or all uridine positions of the nucleic acid and 5-methyl cytidine substitutions at one or more or all cytidine positions of the nucleic acid. 
     In some embodiments, a RNA nucleic acid of the disclosure comprises uridine at one or more or all uridine positions of the nucleic acid. 
     In some embodiments, nucleic acids (e.g., RNA nucleic acids, such as mRNA nucleic acids) are uniformly modified (e.g., fully modified, modified throughout the entire sequence) for a particular modification. For example, a nucleic acid can be uniformly modified with 1-methyl-pseudouridine, meaning that all uridine residues in the mRNA sequence are replaced with 1-methyl-pseudouridine. Similarly, a nucleic acid can be uniformly modified for any type of nucleoside residue present in the sequence by replacement with a modified residue such as those set forth above. 
     The nucleic acids of the present disclosure may be partially or fully modified along the entire length of the molecule. For example, one or more or all or a given type of nucleotide (e.g., purine or pyrimidine, or any one or more or all of A, G, U, C) may be uniformly modified in a nucleic acid of the disclosure, or in a predetermined sequence region thereof (e.g., in the mRNA including or excluding the polyA tail). In some embodiments, all nucleotides X in a nucleic acid of the present disclosure (or in a sequence region thereof) are modified nucleotides, wherein X may be any one of nucleotides A, G, U, C, or any one of the combinations A+G, A+U, A+C, G+U, G+C, U+C, A+G+U, A+G+C, G+U+C or A+G+C. 
     The nucleic acid may contain from about 1% to about 100% modified nucleotides (either in relation to overall nucleotide content, or in relation to one or more types of nucleotide, i.e., any one or more of A, G, U or C) or any intervening percentage (e.g., from 1% to 20%, from 1% to 25%, from 1% to 50%, from 1% to 60%, from 1% to 70%, from 1% to 80%, from 1% to 90%, from 1% to 95%, from 10% to 20%, from 10% to 25%, from 10% to 50%, from 10% to 60%, from 10% to 70%, from 10% to 80%, from 10% to 90%, from 10% to 95%, from 10% to 100%, from 20% to 25%, from 20% to 50%, from 20% to 60%, from 20% to 70%, from 20% to 80%, from 20% to 90%, from 20% to 95%, from 20% to 100%, from 50% to 60%, from 50% to 70%, from 50% to 80%, from 50% to 90%, from 50% to 95%, from 50% to 100%, from 70% to 80%, from 70% to 90%, from 70% to 95%, from 70% to 100%, from 80% to 90%, from 80% to 95%, from 80% to 100%, from 90% to 95%, from 90% to 100%, and from 95% to 100%). It will be understood that any remaining percentage is accounted for by the presence of unmodified A, G, U, or C. 
     The nucleic acids may contain at a minimum 1% and at maximum 100% modified nucleotides, or any intervening percentage, such as at least 5% modified nucleotides, at least 10% modified nucleotides, at least 25% modified nucleotides, at least 50% modified nucleotides, at least 80% modified nucleotides, or at least 90% modified nucleotides. For example, the nucleic acids may contain a modified pyrimidine such as a modified uracil or cytosine. In some embodiments, at least 5%, at least 10%, at least 25%, at least 50%, at least 80%, at least 90% or 100% of the uracil in the nucleic acid is replaced with a modified uracil (e.g., a 5-substituted uracil). The modified uracil can be replaced by a compound having a single unique structure, or can be replaced by a plurality of compounds having different structures (e.g., 2, 3, 4 or more unique structures). In some embodiments, at least 5%, at least 10%, at least 25%, at least 50%, at least 80%, at least 90% or 100% of the cytosine in the nucleic acid is replaced with a modified cytosine (e.g., a 5-substituted cytosine). The modified cytosine can be replaced by a compound having a single unique structure, or can be replaced by a plurality of compounds having different structures (e.g., 2, 3, 4 or more unique structures). 
     Untranslated Regions (UTRs) 
     The nucleic acids of the present disclosure may comprise one or more regions or parts which act or function as an untranslated region. Where nucleic acids are designed to encode at least one antigen of interest, the nucleic may comprise one or more of these untranslated regions (UTRs). Wild-type untranslated regions of a nucleic acid are transcribed but not translated. In mRNA, the 5′ UTR starts at the transcription start site and continues to the start codon but does not include the start codon; whereas, the 3′ UTR starts immediately following the stop codon and continues until the transcriptional termination signal. There is growing body of evidence about the regulatory roles played by the UTRs in terms of stability of the nucleic acid molecule and translation. The regulatory features of a UTR can be incorporated into the polynucleotides of the present disclosure to, among other things, enhance the stability of the molecule. The specific features can also be incorporated to ensure controlled down-regulation of the transcript in case they are misdirected to undesired organs sites. A variety of 5′UTR and 3′UTR sequences are known and available in the art. 
     A 5′ UTR is region of an mRNA that is directly upstream (5′) from the start codon (the first codon of an mRNA transcript translated by a ribosome). A 5′ UTR does not encode a protein (is non-coding). Natural 5′UTRs have features that play roles in translation initiation. They harbor signatures like Kozak sequences which are commonly known to be involved in the process by which the ribosome initiates translation of many genes. Kozak sequences have the consensus CCR(A/G)CCAUGG (SEQ ID NO: 121), where R is a purine (adenine or guanine) three bases upstream of the start codon (AUG), which is followed by another ‘G’.5′UTR also have been known to form secondary structures which are involved in elongation factor binding. 
     In some embodiments of the disclosure, a 5′ UTR is a heterologous UTR, i.e., is a UTR found in nature associated with a different ORF. In another embodiment, a 5′ UTR is a synthetic UTR, i.e., does not occur in nature. Synthetic UTRs include UTRs that have been mutated to improve their properties, e.g., which increase gene expression as well as those which are completely synthetic. Exemplary 5′ UTRs include  Xenopus  or human derived a-globin or b-globin (U.S. Pat. Nos. 8,278,063; 9,012,219), human cytochrome b-245 a polypeptide, and hydroxysteroid (17b) dehydrogenase, and Tobacco etch virus (U.S. Pat. Nos. 8,278,063, 9,012,219). CMV immediate-early 1 (IE1) gene (US20140206753, WO2013/185069), the sequence GGGAUCCUACC (SEQ ID NO: 122) (WO2014/144196) may also be used. In another embodiment, 5′ UTR of a TOP gene is a 5′ UTR of a TOP gene lacking the 5′ TOP motif (the oligopyrimidine tract) (e.g., WO2015/101414, WO2015/101415, WO2015/062738, WO2015/024667, WO2015/024668; 5′ UTR element derived from ribosomal protein Large 32 (L32) gene (WO2015/101414, WO2015/101415, WO2015/062738), 5′ UTR element derived from the 5′UTR of an hydroxysteroid (1743) dehydrogenase 4 gene (HSD17B4) (WO2015/024667), or a 5′ UTR element derived from the 5′ UTR of ATP5A1 (WO2015/024667) can be used. In some embodiments, an internal ribosome entry site (IRES) is used instead of a 5′ UTR. 
     In some embodiments, a 5′ UTR of the present disclosure comprises a sequence selected from SEQ ID NO: 1 and SEQ ID NO: 104. 
     A 3′ UTR is region of an mRNA that is directly downstream (3′) from the stop codon (the codon of an mRNA transcript that signals a termination of translation). A 3′ UTR does not encode a protein (is non-coding). Natural or wild type 3′ UTRs are known to have stretches of adenosines and uridines embedded in them. These AU rich signatures are particularly prevalent in genes with high rates of turnover. Based on their sequence features and functional properties, the AU rich elements (AREs) can be separated into three classes (Chen et al, 1995): Class I AREs contain several dispersed copies of an AUUUA motif within U-rich regions. C-Myc and MyoD contain class I AREs. Class II AREs possess two or more overlapping UUAUUUA(U/A)(U/A) (SEQ ID NO: 123) nonamers. Molecules containing this type of AREs include GM-CSF and TNF-a. Class III ARES are less well defined. These U rich regions do not contain an AUUUA motif. c-Jun and Myogenin are two well-studied examples of this class. Most proteins binding to the AREs are known to destabilize the messenger, whereas members of the ELAV family, most notably HuR, have been documented to increase the stability of mRNA. HuR binds to AREs of all the three classes. Engineering the HuR specific binding sites into the 3′ UTR of nucleic acid molecules will lead to HuR binding and thus, stabilization of the message in vivo. 
     Introduction, removal or modification of 3′ UTR AU rich elements (AREs) can be used to modulate the stability of nucleic acids (e.g., RNA) of the disclosure. When engineering specific nucleic acids, one or more copies of an ARE can be introduced to make nucleic acids of the disclosure less stable and thereby curtail translation and decrease production of the resultant protein. Likewise, AREs can be identified and removed or mutated to increase the intracellular stability and thus increase translation and production of the resultant protein. Transfection experiments can be conducted in relevant cell lines, using nucleic acids of the disclosure and protein production can be assayed at various time points post-transfection. For example, cells can be transfected with different ARE-engineering molecules and by using an ELISA kit to the relevant protein and assaying protein produced at 6 hour, 12 hour, 24 hour, 48 hour, and 7 days post-transfection. 
     3′ UTRs may be heterologous or synthetic. With respect to 3′ UTRs, globin UTRs, including  Xenopus  β-globin UTRs and human β-globin UTRs are known in the art (U.S. Pat. Nos. 8,278,063, 9,012,219, US20110086907). A modified β-globin construct with enhanced stability in some cell types by cloning two sequential human β-globin 3′UTRs head to tail has been developed and is well known in the art (US2012/0195936, WO2014/071963). In addition a2-globin, a1-globin, UTRs and mutants thereof are also known in the art (WO2015/101415, WO2015/024667). Other 3′ UTRs described in the mRNA constructs in the non-patent literature include CYBA (Ferizi et al., 2015) and albumin (Thess et al., 2015). Other exemplary 3′ UTRs include that of bovine or human growth hormone (wild type or modified) (WO2013/185069, US20140206753, WO2014/152774), rabbit βglobin and hepatitis B virus (HBV), α-globin 3′ UTR and Viral VEEV 3′ UTR sequences are also known in the art. In some embodiments, the sequence UUUGAAUU (WO2014/144196) is used. In some embodiments, 3′ UTRs of human and mouse ribosomal protein are used. Other examples include rps9 3′UTR (WO2015/101414), FIG. 4 (WO2015/101415), and human albumin 7 (WO2015/101415). 
     In some embodiments, a 3′ UTR of the present disclosure comprises a sequence selected from SEQ ID NO: 3 and SEQ ID NO: 106. 
     Those of ordinary skill in the art will understand that 5′UTRs that are heterologous or synthetic may be used with any desired 3′ UTR sequence. For example, a heterologous 5′UTR may be used with a synthetic 3′UTR with a heterologous 3″ UTR. 
     Non-UTR sequences may also be used as regions or subregions within a nucleic acid. For example, introns or portions of introns sequences may be incorporated into regions of nucleic acid of the disclosure. Incorporation of intronic sequences may increase protein production as well as nucleic acid levels. 
     Combinations of features may be included in flanking regions and may be contained within other features. For example, the ORF may be flanked by a 5′ UTR which may contain a strong Kozak translational initiation signal and/or a 3′ UTR which may include an oligo(dT) sequence for templated addition of a poly-A tail. 5′ UTR may comprise a first polynucleotide fragment and a second polynucleotide fragment from the same and/or different genes such as the 5′ UTRs described in US Patent Application Publication No. 20100293625 and PCT/US2014/069155, herein incorporated by reference in its entirety. 
     It should be understood that any UTR from any gene may be incorporated into the regions of a nucleic acid. Furthermore, multiple wild-type UTRs of any known gene may be utilized. It is also within the scope of the present disclosure to provide artificial UTRs which are not variants of wild type regions. These UTRs or portions thereof may be placed in the same orientation as in the transcript from which they were selected or may be altered in orientation or location. Hence a 5′ or 3′ UTR may be inverted, shortened, lengthened, made with one or more other 5′ UTRs or 3′ UTRs. As used herein, the term “altered” as it relates to a UTR sequence, means that the UTR has been changed in some way in relation to a reference sequence. For example, a 3′ UTR or 5′ UTR may be altered relative to a wild-type or native UTR by the change in orientation or location as taught above or may be altered by the inclusion of additional nucleotides, deletion of nucleotides, swapping or transposition of nucleotides. Any of these changes producing an “altered” UTR (whether 3′ or 5′) comprise a variant UTR. 
     In some embodiments, a double, triple or quadruple UTR such as a 5′ UTR or 3′ UTR may be used. As used herein, a “double” UTR is one in which two copies of the same UTR are encoded either in series or substantially in series. For example, a double beta-globin 3′ UTR may be used as described in US Patent publication 20100129877, the contents of which are incorporated herein by reference in its entirety. 
     It is also within the scope of the present disclosure to have patterned UTRs. As used herein “patterned UTRs” are those UTRs which reflect a repeating or alternating pattern, such as ABABAB or AABBAABBAABB or ABCABCABC or variants thereof repeated once, twice, or more than 3 times. In these patterns, each letter, A, B, or C represent a different UTR at the nucleotide level. 
     In some embodiments, flanking regions are selected from a family of transcripts whose proteins share a common function, structure, feature or property. For example, polypeptides of interest may belong to a family of proteins which are expressed in a particular cell, tissue or at some time during development. The UTRs from any of these genes may be swapped for any other UTR of the same or different family of proteins to create a new polynucleotide. As used herein, a “family of proteins” is used in the broadest sense to refer to a group of two or more polypeptides of interest which share at least one function, structure, feature, localization, origin, or expression pattern. 
     The untranslated region may also include translation enhancer elements (TEE). As a non-limiting example, the TEE may include those described in US Application No. 20090226470, herein incorporated by reference in its entirety, and those known in the art. 
     In Vitro Transcription of RNA 
     cDNA encoding the polynucleotides described herein may be transcribed using an in vitro transcription (IVT) system. In vitro transcription of RNA is known in the art and is described in International Publication WO/2014/152027, which is incorporated by reference herein in its entirety. 
     In some embodiments, the RNA transcript is generated using a non-amplified, linearized DNA template in an in vitro transcription reaction to generate the RNA transcript. In some embodiments, the template DNA is isolated DNA. In some embodiments, the template DNA is cDNA. In some embodiments, the cDNA is formed by reverse transcription of a RNA polynucleotide, for example, but not limited to EBV RNA, e.g. EBV mRNA. In some embodiments, cells, e.g., bacterial cells, e.g.,  E. coli , e.g., DH-1 cells are transfected with the plasmid DNA template. In some embodiments, the transfected cells are cultured to replicate the plasmid DNA which is then isolated and purified. In some embodiments, the DNA template includes a RNA polymerase promoter, e.g., a T7 promoter located 5′ to and operably linked to the gene of interest. 
     In some embodiments, an in vitro transcription template encodes a 5′ untranslated (UTR) region, contains an open reading frame, and encodes a 3′ UTR and a polyA tail. The particular nucleic acid sequence composition and length of an in vitro transcription template will depend on the mRNA encoded by the template. 
     A “5′ untranslated region” (UTR) refers to a region of an mRNA that is directly upstream (i.e., 5′) from the start codon (i.e., the first codon of an mRNA transcript translated by a ribosome) that does not encode a polypeptide. When RNA transcripts are being generated, the 5′ UTR may comprise a promoter sequence. Such promoter sequences are known in the art. It should be understood that such promoter sequences will not be present in a vaccine of the disclosure. 
     A “3′ untranslated region” (UTR) refers to a region of an mRNA that is directly downstream (i.e., 3′) from the stop codon (i.e., the codon of an mRNA transcript that signals a termination of translation) that does not encode a polypeptide. 
     An “open reading frame” is a continuous stretch of DNA beginning with a start codon (e.g., methionine (ATG)), and ending with a stop codon (e.g., TAA, TAG or TGA) and encodes a polypeptide. 
     A “polyA tail” is a region of mRNA that is downstream, e.g., directly downstream (i.e., 3′), from the 3′ UTR that contains multiple, consecutive adenosine monophosphates. A polyA tail may contain 10 to 300 adenosine monophosphates. For example, a polyA tail may contain 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290 or 300 adenosine monophosphates. In some embodiments, a polyA tail contains 50 to 250 adenosine monophosphates. In a relevant biological setting (e.g., in cells, in vivo) the poly(A) tail functions to protect mRNA from enzymatic degradation, e.g., in the cytoplasm, and aids in transcription termination, and/or export of the mRNA from the nucleus and translation. 
     In some embodiments, a nucleic acid includes 200 to 3,000 nucleotides. For example, a nucleic acid may include 200 to 500, 200 to 1000, 200 to 1500, 200 to 3000, 500 to 1000, 500 to 1500, 500 to 2000, 500 to 3000, 1000 to 1500, 1000 to 2000, 1000 to 3000, 1500 to 3000, or 2000 to 3000 nucleotides). 
     An in vitro transcription system typically comprises a transcription buffer, nucleotide triphosphates (NTPs), an RNase inhibitor and a polymerase. 
     The NTPs may be manufactured in house, may be selected from a supplier, or may be synthesized as described herein. The NTPs may be selected from, but are not limited to, those described herein including natural and unnatural (modified) NTPs. 
     Any number of RNA polymerases or variants may be used in the method of the present disclosure. The polymerase may be selected from, but is not limited to, a phage RNA polymerase, e.g., a T7 RNA polymerase, a T3 RNA polymerase, a SP6 RNA polymerase, and/or mutant polymerases such as, but not limited to, polymerases able to incorporate modified nucleic acids and/or modified nucleotides, including chemically modified nucleic acids and/or nucleotides. Some embodiments exclude the use of DNase. 
     In some embodiments, the RNA transcript is capped via enzymatic capping. In some embodiments, the RNA comprises 5′ terminal cap, for example, 7mG(5′)ppp(5′)NlmpNp. 
     Chemical Synthesis 
     Solid-Phase Chemical Synthesis. 
     Nucleic acids the present disclosure may be manufactured in whole or in part using solid phase techniques. Solid-phase chemical synthesis of nucleic acids is an automated method wherein molecules are immobilized on a solid support and synthesized step by step in a reactant solution. Solid-phase synthesis is useful in site-specific introduction of chemical modifications in the nucleic acid sequences. 
     Liquid Phase Chemical Synthesis. 
     The synthesis of nucleic acids of the present disclosure by the sequential addition of monomer building blocks may be carried out in a liquid phase. 
     Combination of Synthetic Methods. 
     The synthetic methods discussed above each has its own advantages and limitations. Attempts have been conducted to combine these methods to overcome the limitations. Such combinations of methods are within the scope of the present disclosure. The use of solid-phase or liquid-phase chemical synthesis in combination with enzymatic ligation provides an efficient way to generate long chain nucleic acids that cannot be obtained by chemical synthesis alone. 
     Ligation of Nucleic Acid Regions or Subregions 
     Assembling nucleic acids by a ligase may also be used. DNA or RNA ligases promote intermolecular ligation of the 5′ and 3′ ends of polynucleotide chains through the formation of a phosphodiester bond. Nucleic acids such as chimeric polynucleotides and/or circular nucleic acids may be prepared by ligation of one or more regions or subregions. DNA fragments can be joined by a ligase catalyzed reaction to create recombinant DNA with different functions. Two oligodeoxynucleotides, one with a 5′ phosphoryl group and another with a free 3′ hydroxyl group, serve as substrates for a DNA ligase. 
     Purification 
     Purification of the nucleic acids described herein may include, but is not limited to, nucleic acid clean-up, quality assurance and quality control. Clean-up may be performed by methods known in the arts such as, but not limited to, AGENCOURT® beads (Beckman Coulter Genomics, Danvers, Mass.), poly-T beads, LNATM oligo-T capture probes (EXIQON® Inc, Vedbaek, Denmark) or HPLC based purification methods such as, but not limited to, strong anion exchange HPLC, weak anion exchange HPLC, reverse phase HPLC (RP-HPLC), and hydrophobic interaction HPLC (HIC-HPLC). The term “purified” when used in relation to a nucleic acid such as a “purified nucleic acid” refers to one that is separated from at least one contaminant. A “contaminant” is any substance that makes another unfit, impure or inferior. Thus, a purified nucleic acid (e.g., DNA and RNA) is present in a form or setting different from that in which it is found in nature, or a form or setting different from that which existed prior to subjecting it to a treatment or purification method. 
     A quality assurance and/or quality control check may be conducted using methods such as, but not limited to, gel electrophoresis, UV absorbance, or analytical HPLC. 
     In some embodiments, the nucleic acids may be sequenced by methods including, but not limited to reverse-transcriptase-PCR. 
     Quantification 
     In some embodiments, the nucleic acids of the present invention may be quantified in exosomes or when derived from one or more bodily fluid. Bodily fluids include peripheral blood, serum, plasma, ascites, urine, cerebrospinal fluid (CSF), sputum, saliva, bone marrow, synovial fluid, aqueous humor, amniotic fluid, cerumen, breast milk, broncheoalveolar lavage fluid, semen, prostatic fluid, cowper&#39;s fluid or pre-ejaculatory fluid, sweat, fecal matter, hair, tears, cyst fluid, pleural and peritoneal fluid, pericardial fluid, lymph, chyme, chyle, bile, interstitial fluid, menses, pus, sebum, vomit, vaginal secretions, mucosal secretion, stool water, pancreatic juice, lavage fluids from sinus cavities, bronchopulmonary aspirates, blastocyl cavity fluid, and umbilical cord blood. Alternatively, exosomes may be retrieved from an organ selected from the group consisting of lung, heart, pancreas, stomach, intestine, bladder, kidney, ovary, testis, skin, colon, breast, prostate, brain, esophagus, liver, and placenta. 
     Assays may be performed using construct specific probes, cytometry, qRT-PCR, real-time PCR, PCR, flow cytometry, electrophoresis, mass spectrometry, or combinations thereof while the exosomes may be isolated using immunohistochemical methods such as enzyme linked immunosorbent assay (ELISA) methods. Exosomes may also be isolated by size exclusion chromatography, density gradient centrifugation, differential centrifugation, nanomembrane ultrafiltration, immunoabsorbent capture, affinity purification, microfluidic separation, or combinations thereof. 
     These methods afford the investigator the ability to monitor, in real time, the level of nucleic acids remaining or delivered. This is possible because the nucleic acids of the present disclosure, in some embodiments, differ from the endogenous forms due to the structural or chemical modifications. 
     In some embodiments, the nucleic acid may be quantified using methods such as, but not limited to, ultraviolet visible spectroscopy (UV/Vis). A non-limiting example of a UV/Vis spectrometer is a NANODROP® spectrometer (ThermoFisher, Waltham, Mass.). The quantified nucleic acid may be analyzed in order to determine if the nucleic acid may be of proper size, check that no degradation of the nucleic acid has occurred. Degradation of the nucleic acid may be checked by methods such as, but not limited to, agarose gel electrophoresis, HPLC based purification methods such as, but not limited to, strong anion exchange HPLC, weak anion exchange HPLC, reverse phase HPLC (RP-HPLC), and hydrophobic interaction HPLC (HIC-HPLC), liquid chromatography-mass spectrometry (LCMS), capillary electrophoresis (CE) and capillary gel electrophoresis (CGE). 
     Pharmaceutical Formulations 
     Provided herein are compositions (e.g., pharmaceutical compositions), methods, kits and reagents for prevention or treatment of EBV in humans and other mammals, for example. EBV RNA (e.g., mRNA) vaccines can be used as therapeutic or prophylactic agents. They may be used in medicine to prevent and/or treat infectious disease. 
     In some embodiments, an EBV vaccine containing RNA polynucleotides as described herein can be administered to a subject (e.g., a mammalian subject, such as a human subject), and the RNA polynucleotides are translated in vivo to produce an antigenic polypeptide (antigen). 
     An “effective amount” of an EBV vaccine is based, at least in part, on the target tissue, target cell type, means of administration, physical characteristics of the RNA (e.g., length, nucleotide composition, and/or extent of modified nucleosides), other components of the vaccine, and other determinants, such as age, body weight, height, sex and general health of the subject. Typically, an effective amount of an EBV vaccine provides an induced or boosted immune response as a function of antigen production in the cells of the subject. In some embodiments, an effective amount of the EBV RNA vaccine containing RNA polynucleotides having at least one chemical modifications are more efficient than a composition containing a corresponding unmodified polynucleotide encoding the same antigen or a peptide antigen. Increased antigen production may be demonstrated by increased cell transfection (the percentage of cells transfected with the RNA vaccine), increased protein translation and/or expression from the polynucleotide, decreased nucleic acid degradation (as demonstrated, for example, by increased duration of protein translation from a modified polynucleotide), or altered antigen specific immune response of the host cell. 
     The term “pharmaceutical composition” refers to the combination of an active agent with a carrier, inert or active, making the composition especially suitable for diagnostic or therapeutic use in vivo or ex vivo. A “pharmaceutically acceptable carrier,” after administered to or upon a subject, does not cause undesirable physiological effects. The carrier in the pharmaceutical composition must be “acceptable” also in the sense that it is compatible with the active ingredient and can be capable of stabilizing it. One or more solubilizing agents can be utilized as pharmaceutical carriers for delivery of an active agent. Examples of a pharmaceutically acceptable carrier include, but are not limited to, biocompatible vehicles, adjuvants, additives, and diluents to achieve a composition usable as a dosage form. Examples of other carriers include colloidal silicon oxide, magnesium stearate, cellulose, and sodium lauryl sulfate. Additional suitable pharmaceutical carriers and diluents, as well as pharmaceutical necessities for their use, are described in Remington&#39;s Pharmaceutical Sciences. 
     In some embodiments, RNA vaccines (including polynucleotides and their encoded polypeptides) in accordance with the present disclosure may be used for treatment or prevention of EBV. EBV RNA vaccines may be administered prophylactically or therapeutically as part of an active immunization scheme to healthy individuals or early in infection during the incubation phase or during active infection after onset of symptoms. In some embodiments, the amount of RNA vaccines of the present disclosure provided to a cell, a tissue or a subject may be an amount effective for immune prophylaxis. 
     EBV RNA (e.g., mRNA) vaccines may be administered with other prophylactic or therapeutic compounds. As a non-limiting example, a prophylactic or therapeutic compound may be an adjuvant or a booster. As used herein, when referring to a prophylactic composition, such as a vaccine, the term “booster” refers to an extra administration of the prophylactic (vaccine) composition. A booster (or booster vaccine) may be given after an earlier administration of the prophylactic composition. The time of administration between the initial administration of the prophylactic composition and the booster may be, but is not limited to, 1 minute, 2 minutes, 3 minutes, 4 minutes, 5 minutes, 6 minutes, 7 minutes, 8 minutes, 9 minutes, 10 minutes, 15 minutes, 20 minutes 35 minutes, 40 minutes, 45 minutes, 50 minutes, 55 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, 11 hours, 12 hours, 13 hours, 14 hours, 15 hours, 16 hours, 17 hours, 18 hours, 19 hours, 20 hours, 21 hours, 22 hours, 23 hours, 1 day, 36 hours, 2 days, 3 days, 4 days, 5 days, 6 days, 1 week, 10 days, 2 weeks, 3 weeks, 1 month, 2 months, 3 months, 4 months, 5 months, 6 months, 7 months, 8 months, 9 months, 10 months, 11 months, 1 year, 18 months, 2 years, 3 years, 4 years, 5 years, 6 years, 7 years, 8 years, 9 years, 10 years, 11 years, 12 years, 13 years, 14 years, 15 years, 16 years, 17 years, 18 years, 19 years, 20 years, 25 years, 30 years, 35 years, 40 years, 45 years, 50 years, 55 years, 60 years, 65 years, 70 years, 75 years, 80 years, 85 years, 90 years, 95 years or more than 99 years. In exemplary embodiments, the time of administration between the initial administration of the prophylactic composition and the booster may be, but is not limited to, 1 week, 2 weeks, 3 weeks, 1 month, 2 months, 3 months, 6 months or 1 year. 
     In some embodiments, EBV RNA vaccines may be administered intramuscularly, intranasally or intradermally, similarly to the administration of inactivated vaccines known in the art. 
     The EBV RNA vaccines may be utilized in various settings depending on the prevalence of the infection or the degree or level of unmet medical need. As a non-limiting example, the RNA vaccines may be utilized to treat and/or prevent a variety of infectious disease. RNA vaccines have superior properties in that they produce much larger antibody titers, better neutralizing immunity, produce more durable immune responses, and/or produce responses earlier than commercially available vaccines. 
     Provided herein are pharmaceutical compositions including EBV RNA vaccines and RNA vaccine compositions and/or complexes optionally in combination with one or more pharmaceutically acceptable excipients. 
     EBV RNA (e.g., mRNA) vaccines may be formulated or administered alone or in conjunction with one or more other components. For instance, EBV RNA vaccines (vaccine compositions) may comprise other components including, but not limited to, adjuvants. 
     In some embodiments, EBV RNA vaccines do not include an adjuvant (they are adjuvant free). 
     EBV RNA (e.g., mRNA) vaccines may be formulated or administered in combination with one or more pharmaceutically-acceptable excipients. In some embodiments, vaccine compositions comprise at least one additional active substances, such as, for example, a therapeutically-active substance, a prophylactically-active substance, or a combination of both. Vaccine compositions may be sterile, pyrogen-free or both sterile and pyrogen-free. General considerations in the formulation and/or manufacture of pharmaceutical agents, such as vaccine compositions, may be found, for example, in Remington: The Science and Practice of Pharmacy 21st ed., Lippincott Williams &amp; Wilkins, 2005 (incorporated herein by reference in its entirety). 
     In some embodiments, EBV RNA vaccines are administered to humans, human patients or subjects. For the purposes of the present disclosure, the phrase “active ingredient” generally refers to the RNA vaccines or the polynucleotides contained therein, for example, RNA polynucleotides (e.g., mRNA polynucleotides) encoding antigens. 
     Formulations of the vaccine compositions described herein may be prepared by any method known or hereafter developed in the art of pharmacology. In general, such preparatory methods include the step of bringing the active ingredient (e.g., mRNA polynucleotide) into association with an excipient and/or one or more other accessory ingredients, and then, if necessary and/or desirable, dividing, shaping and/or packaging the product into a desired single- or multi-dose unit. 
     Relative amounts of the active ingredient, the pharmaceutically acceptable excipient, and/or any additional ingredients in a pharmaceutical composition in accordance with the disclosure will vary, depending upon the identity, size, and/or condition of the subject treated and further depending upon the route by which the composition is to be administered. By way of example, the composition may comprise between 0.1% and 100%, e.g., between 0.5 and 50%, between 1-30%, between 5-80%, at least 80% (w/w) active ingredient. 
     In some embodiments, EBV RNA vaccines are formulated using one or more excipients to: (1) increase stability; (2) increase cell transfection; (3) permit the sustained or delayed release (e.g., from a depot formulation); (4) alter the biodistribution (e.g., target to specific tissues or cell types); (5) increase the translation of encoded protein in vivo; and/or (6) alter the release profile of encoded protein (antigen) in vivo. In addition to traditional excipients such as any and all solvents, dispersion media, diluents, or other liquid vehicles, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, excipients can include, without limitation, lipidoids, liposomes, lipid nanoparticles, polymers, lipoplexes, core-shell nanoparticles, peptides, proteins, cells transfected with EBV RNA vaccines (e.g., for transplantation into a subject), hyaluronidase, nanoparticle mimics and combinations thereof. 
     Lipid Nanoparticles (LNPs) 
     In some embodiments, EBV RNA (e.g., mRNA) vaccines of the disclosure are formulated in a lipid nanoparticle (LNP). Lipid nanoparticles typically comprise ionizable cationic lipid, non-cationic lipid, sterol and PEG lipid components along with the nucleic acid cargo of interest. The lipid nanoparticles of the disclosure can be generated using components, compositions, and methods as are generally known in the art, see for example PCT/US2016/052352; PCT/US2016/068300; PCT/US2017/037551; PCT/US2015/027400; PCT/US2016/047406; PCT/US2016000129; PCT/US2016/014280; PCT/US2016/014280; PCT/US2017/038426; PCT/US2014/027077; PCT/US2014/055394; PCT/US2016/52117; PCT/US2012/069610; PCT/US2017/027492; PCT/US2016/059575 and PCT/US2016/069491 all of which are incorporated by reference herein in their entirety. 
     Vaccines of the present disclosure are typically formulated in lipid nanoparticle. In some embodiments, the lipid nanoparticle comprises at least one ionizable cationic lipid, at least one non-cationic lipid, at least one sterol, and/or at least one polyethylene glycol (PEG)-modified lipid. 
     In some embodiments, the lipid nanoparticle comprises a molar ratio of 20-60% ionizable cationic lipid. For example, the lipid nanoparticle may comprise a molar ratio of 20-50%, 20-40%, 20-30%, 30-60%, 30-50%, 30-40%, 40-60%, 40-50%, or 50-60% ionizable cationic lipid. In some embodiments, the lipid nanoparticle comprises a molar ratio of 20%, 30%, 40%, 50, or 60% ionizable cationic lipid. 
     In some embodiments, the lipid nanoparticle comprises a molar ratio of 5-25% non-cationic lipid. For example, the lipid nanoparticle may comprise a molar ratio of 5-20%, 5-15%, 5-10%, 10-25%, 10-20%, 10-25%, 15-25%, 15-20%, or 20-25% non-cationic lipid. In some embodiments, the lipid nanoparticle comprises a molar ratio of 5%, 10%, 15%, 20%, or25% non-cationic lipid. 
     In some embodiments, the lipid nanoparticle comprises a molar ratio of 25-55% sterol. For example, the lipid nanoparticle may comprise a molar ratio of 25-50%, 25-45%, 25-40%, 25-35%, 25-30%, 30-55%, 30-50%, 30-45%, 30-40%, 30-35%, 35-55%, 35-50%, 35-45%, 35-40%, 40-55%, 40-50%, 40-45%, 45-55%, 45-50%, or 50-55% sterol. In some embodiments, the lipid nanoparticle comprises a molar ratio of 25%, 30%, 35%, 40%, 45%, 50%, or 55% sterol. 
     In some embodiments, the lipid nanoparticle comprises a molar ratio of 0.5-15% PEG-modified lipid. For example, the lipid nanoparticle may comprise a molar ratio of 0.5-10%, 0.5-5%, 1-15%, 1-10%, 1-5%, 2-15%, 2-10%, 2-5%, 5-15%, 5-10%, or 10-15%. In some embodiments, the lipid nanoparticle comprises a molar ratio of 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, or 15% PEG-modified lipid. 
     In some embodiments, the lipid nanoparticle comprises a molar ratio of 20-60% ionizable cationic lipid, 5-25% non-cationic lipid, 25-55% sterol, and 0.5-15% PEG-modified lipid. 
     In some embodiments, an ionizable cationic lipid of the disclosure comprises a compound of Formula (I): 
     
       
         
         
             
             
         
       
     
     or a salt or isomer thereof, wherein: 
     R 1  is selected from the group consisting of C 5-30  alkyl, C 5-20  alkenyl, —R*YR″, —YR″, and —R″M′R′; 
     R 2  and R 3  are independently selected from the group consisting of H, C 1-14  alkyl, C 2-14  alkenyl, —R*YR″, —YR″, and —R*OR″, or R 2  and R 3 , together with the atom to which they are attached, form a heterocycle or carbocycle; 
     R 4  is selected from the group consisting of a C 3-6  carbocycle, —(CH 2 ) n Q, —(CH 2 ) n CHQR, —CHQR, —CQ(R) 2 , and unsubstituted C 1-6  alkyl, where Q is selected from a carbocycle, heterocycle, —OR, —O(CH 2 ) n N(R) 2 , —C(O)OR, —OC(O)R, —CX 3 , —CX 2 H, —CXH 2 , —CN, —N(R) 2 , —C(O)N(R) 2 , —N(R)C(O)R, —N(R)S(O) 2 R, —N(R)C(O)N(R) 2 , —N(R)C(S)N(R) 2 , —N(R)R 8 , —O(CH 2 ) n OR, —N(R)C(═NR 9 )N(R) 2 , —N(R)C(═CHR 9 )N(R) 2 , —OC(O)N(R) 2 , —N(R)C(O)OR, —N(OR)C(O)R, —N(OR)S(O) 2 R, —N(OR)C(O)OR, —N(OR)C(O)N(R) 2 , —N(OR)C(S)N(R) 2 , —N(OR)C(═NR 9 )N(R) 2 , —N(OR)C(═CHR 9 )N(R) 2 , —C(═NR 9 )N(R) 2 , —C(═NR 9 )R, —C(O)N(R)OR, and —C(R)N(R) 2 C(O)OR, and each n is independently selected from 1, 2, 3, 4, and 5; 
     each R 5  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R 6  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     M and M′ are independently selected from —C(O)O—, —OC(O)—, —C(O)N(R′)—, —N(R′)C(O)—, —C(O)—, —C(S)—, —C(S)S—, —SC(S)—, —CH(OH)—, —P(O)(OR′)O—, —S(O) 2 —, —S—S—, an aryl group, and a heteroaryl group; 
     R 7  is selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     R 8  is selected from the group consisting of C 3-6  carbocycle and heterocycle; 
     R 9  is selected from the group consisting of H, CN, NO 2 , C 1-6  alkyl, —OR, —S(O) 2 R, —S(O) 2 N(R) 2 , C 2-6  alkenyl, C 3-6  carbocycle and heterocycle; 
     each R is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R′ is independently selected from the group consisting of C 1-18  alkyl, C 2-18  alkenyl, —R*YR″, —YR″, and H; 
     each R″ is independently selected from the group consisting of C 3-14  alkyl and C 3-14  alkenyl; 
     each R* is independently selected from the group consisting of C 1-12  alkyl and C 2-12  alkenyl; 
     each Y is independently a C 3-6  carbocycle; 
     each X is independently selected from the group consisting of F, Cl, Br, and I; and 
     m is selected from 5, 6, 7, 8, 9, 10, 11, 12, and 13. 
     In some embodiments, a subset of compounds of Formula (I) includes those in which when R 4  is —(CH 2 ) n Q, —(CH 2 ) n CHQR, —CHQR, or —CQ(R) 2 , then (i) Q is not —N(R) 2  when n is 1, 2, 3, 4 or 5, or (ii) Q is not 5, 6, or 7-membered heterocycloalkyl when n is 1 or 2. 
     In some embodiments, another subset of compounds of Formula (I) includes those in which 
     R 1  is selected from the group consisting of C 5-30  alkyl, C 5-20  alkenyl, —R*YR″, —YR″, and —R″M′R′; 
     R 2  and R 3  are independently selected from the group consisting of H, C 1-14  alkyl, C 2-14  alkenyl, —R*YR″, —YR″, and —R*OR″, or R 2  and R 3 , together with the atom to which they are attached, form a heterocycle or carbocycle; 
     R 4  is selected from the group consisting of a C 3-6  carbocycle, —(CH 2 ) n Q, —(CH 2 ) n CHQR, —CHQR, —CQ(R) 2 , and unsubstituted C 1-6  alkyl, where Q is selected from a C 3-6  carbocycle, a 5- to 14-membered heteroaryl having one or more heteroatoms selected from N, O, and S, —OR, —(CH 2 ) n N(R) 2 , —C(O)OR, —OC(O)R, —CX 3 , —CX 2 H, —CXH 2 , —CN, —C(O)N(R) 2 , —N(R)C(O)R, —N(R)S(O) 2 R, —N(R)C(O)N(R) 2 , —N(R)C(S)N(R) 2 , —CRN(R) 2 C(O)OR, —N(R)R 8 , —O(CH 2 ) n OR, —N(R)C(═NR 9 )N(R) 2 , —N(R)C(═CHR 9 )N(R) 2 , —OC(O)N(R) 2 , —N(R)C(O)OR, —N(OR)C(O)R, —N(OR)S(O) 2 R, —N(OR)C(O)OR, —N(OR)C(O)N(R) 2 , —N(OR)C(S)N(R) 2 , —N(OR)C(═NR 9 )N(R) 2 , —N(OR)C(═CHR 9 )N(R) 2 , —C(═NR 9 )N(R) 2 , —C(═NR 9 )R, —C(O)N(R)OR, and a 5- to 14-membered heterocycloalkyl having one or more heteroatoms selected from N, O, and S which is substituted with one or more substituents selected from oxo (═O), OH, amino, mono- or di-alkylamino, and C 1-3  alkyl, and each n is independently selected from 1, 2, 3, 4, and 5; 
     each R 5  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R 6  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     M and M′ are independently selected from —C(O)O—, —OC(O)—, —C(O)N(R′)—, —N(R′)C(O)—, —C(O)—, —C(S)—, —C(S)S—, —SC(S)—, —CH(OH)—, —P(O)(OR′)O—, —S(O) 2 —, —S—S—, an aryl group, and a heteroaryl group; 
     R 7  is selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     R 8  is selected from the group consisting of C 3-6  carbocycle and heterocycle; 
     R 9  is selected from the group consisting of H, CN, NO 2 , C 1-6  alkyl, —OR, —S(O) 2 R, —S(O) 2 N(R) 2 , C 2-6  alkenyl, C 3-6  carbocycle and heterocycle; 
     each R is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R′ is independently selected from the group consisting of C 1-18  alkyl, C 2-18  alkenyl, —R*YR″, —YR″, and H; 
     each R″ is independently selected from the group consisting of C 3-14  alkyl and C 3-14  alkenyl; 
     each R* is independently selected from the group consisting of C 1-12  alkyl and C 2-12  alkenyl; 
     each Y is independently a C 3-6  carbocycle; 
     each X is independently selected from the group consisting of F, Cl, Br, and I; and 
     m is selected from 5, 6, 7, 8, 9, 10, 11, 12, and 13, 
     or salts or isomers thereof. 
     In some embodiments, another subset of compounds of Formula (I) includes those in which 
     R 1  is selected from the group consisting of C 5-30  alkyl, C 5-20  alkenyl, —R*YR″, —YR″, and —R″M′R′; 
     R 2  and R 3  are independently selected from the group consisting of H, C 1-14  alkyl, C 2-14  alkenyl, —R*YR″, —YR″, and —R*OR″, or R 2  and R 3 , together with the atom to which they are attached, form a heterocycle or carbocycle; 
     R 4  is selected from the group consisting of a C 3-6  carbocycle, —(CH 2 ) n Q, —(CH 2 ) n CHQR, —CHQR, —CQ(R) 2 , and unsubstituted C 1-6  alkyl, where Q is selected from a C 3-6  carbocycle, a 5- to 14-membered heterocycle having one or more heteroatoms selected from N, O, and S, —OR, —O(CH 2 ) n N(R) 2 , —C(O)OR, —OC(O)R, —CX 3 , —CX 2 H, —CXH 2 , —CN, —C(O)N(R) 2 , —N(R)C(O)R, —N(R)S(O) 2 R, —N(R)C(O)N(R) 2 , —N(R)C(S)N(R) 2 , —CRN(R) 2 C(O)OR, —N(R)R 8 , —O(CH 2 ) n OR, —N(R)C(═NR 9 )N(R) 2 , —N(R)C(═CHR 9 )N(R) 2 , —OC(O)N(R) 2 , —N(R)C(O)OR, —N(OR)C(O)R, —N(OR)S(O) 2 R, —N(OR)C(O)OR, —N(OR)C(O)N(R) 2 , —N(OR)C(S)N(R) 2 , —N(OR)C(═NR 9 )N(R) 2 , —N(OR)C(═CHR 9 )N(R) 2 , —C(═NR 9 )R, —C(O)N(R)OR, and —C(═NR 9 )N(R) 2 , and each n is independently selected from 1, 2, 3, 4, and 5; and when Q is a 5- to 14-membered heterocycle and (i) R 4  is —(CH 2 ) n Q in which n is 1 or 2, or (ii) R 4  is —(CH 2 ) n CHQR in which n is 1, or (iii) R 4  is —CHQR, and —CQ(R) 2 , then Q is either a 5- to 14-membered heteroaryl or 8- to 14-membered heterocycloalkyl; 
     each R 5  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R 6  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     M and M′ are independently selected from —C(O)O—, —OC(O)—, —C(O)N(R′)—, —N(R′)C(O)—, —C(O)—, —C(S)—, —C(S)S—, —SC(S)—, —CH(OH)—, —P(O)(OR′)O—, —S(O) 2 —, —S—S—, an aryl group, and a heteroaryl group; 
     R 7  is selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     R 8  is selected from the group consisting of C 3-6  carbocycle and heterocycle; 
     R 9  is selected from the group consisting of H, CN, NO 2 , C 1-6  alkyl, —OR, —S(O) 2 R, —S(O) 2 N(R) 2 , C 2-6  alkenyl, C 3-6  carbocycle and heterocycle; 
     each R is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R′ is independently selected from the group consisting of C 1-18  alkyl, C 2-18  alkenyl, —R*YR″, —YR″, and H; 
     each R″ is independently selected from the group consisting of C 3-14  alkyl and C 3-14  alkenyl; 
     each R* is independently selected from the group consisting of C 1-12  alkyl and C 2-12  alkenyl; 
     each Y is independently a C 3-6  carbocycle; 
     each X is independently selected from the group consisting of F, Cl, Br, and I; and 
     m is selected from 5, 6, 7, 8, 9, 10, 11, 12, and 13, 
     or salts or isomers thereof. 
     In some embodiments, another subset of compounds of Formula (I) includes those in which 
     R 1  is selected from the group consisting of C 5-30  alkyl, C 5-20  alkenyl, —R*YR″, —YR″, and —R″M′R′; 
     R 2  and R 3  are independently selected from the group consisting of H, C 1-14  alkyl, C 2-14  alkenyl, —R*YR″, —YR″, and —R*OR″, or R 2  and R 3 , together with the atom to which they are attached, form a heterocycle or carbocycle; 
     R 4  is selected from the group consisting of a C 3-6  carbocycle, —(CH 2 ) n Q, —(CH 2 ) n CHQR, —CHQR, —CQ(R) 2 , and unsubstituted C 1-6  alkyl, where Q is selected from a C 3-6  carbocycle, a 5- to 14-membered heteroaryl having one or more heteroatoms selected from N, O, and S, —OR, —O(CH 2 ) n N(R) 2 , —C(O)OR, —OC(O)R, —CX 3 , —CX 2 H, —CXH 2 , —CN, —C(O)N(R) 2 , —N(R)C(O)R, —N(R)S(O) 2 R, —N(R)C(O)N(R) 2 , —N(R)C(S)N(R) 2 , —CRN(R) 2 C(O)OR, —N(R)R 8 , —O(CH 2 ) n OR, —N(R)C(═NR 9 )N(R) 2 , —N(R)C(═CHR 9 )N(R) 2 , —OC(O)N(R) 2 , —N(R)C(O)OR, —N(OR)C(O)R, —N(OR)S(O) 2 R, —N(OR)C(O)OR, —N(OR)C(O)N(R) 2 , —N(OR)C(S)N(R) 2 , —N(OR)C(═NR 9 )N(R) 2 , —N(OR)C(═CHR 9 )N(R) 2 , —C(═NR 9 )R, —C(O)N(R)OR, and —C(═NR 9 )N(R) 2 , and each n is independently selected from 1, 2, 3, 4, and 5; 
     each R 5  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R 6  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     M and M′ are independently selected from —C(O)O—, —OC(O)—, —C(O)N(R′)—, —N(R′)C(O)—, —C(O)—, —C(S)—, —C(S)S—, —SC(S)—, —CH(OH)—, —P(O)(OR′)O—, —S(O) 2 —, —S—S—, an aryl group, and a heteroaryl group; 
     R 7  is selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     R 8  is selected from the group consisting of C 3-6  carbocycle and heterocycle; 
     R 9  is selected from the group consisting of H, CN, NO2, C 1-6  alkyl, —OR, —S(O) 2 R, —S(O) 2 N(R) 2 , C 2-6  alkenyl, C 3-6  carbocycle and heterocycle; 
     each R is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R′ is independently selected from the group consisting of C 1-18  alkyl, C 2-18  alkenyl, —R*YR″, —YR″, and H; 
     each R″ is independently selected from the group consisting of C 3-14  alkyl and C 3-14  alkenyl; 
     each R* is independently selected from the group consisting of C 1-12  alkyl and C 2-12  alkenyl; 
     each Y is independently a C 3-6  carbocycle; 
     each X is independently selected from the group consisting of F, Cl, Br, and I; and 
     m is selected from 5, 6, 7, 8, 9, 10, 11, 12, and 13, 
     or salts or isomers thereof. 
     In some embodiments, another subset of compounds of Formula (I) includes those in which 
     R 1  is selected from the group consisting of C 5-30  alkyl, C 5-20  alkenyl, —R*YR″, —YR″, and —R″M′R′; 
     R 2  and R 3  are independently selected from the group consisting of H, C 2-14  alkyl, C 2-14  alkenyl, —R*YR″, —YR″, and —R*OR″, or R 2  and R 3 , together with the atom to which they are attached, form a heterocycle or carbocycle; 
     R 4  is —(CH 2 ) n Q or —(CH 2 ) n CHQR, where Q is —N(R) 2 , and n is selected from 3, 4, and 5; 
     each R 5  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R 6  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     M and M′ are independently selected from —C(O)O—, —OC(O)—, —C(O)N(R′)—, —N(R′)C(O)—, —C(O)—, —C(S)—, —C(S)S—, —SC(S)—, —CH(OH)—, —P(O)(OR′)O—, —S(O) 2 —, —S—S—, an aryl group, and a heteroaryl group; 
     R 7  is selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R′ is independently selected from the group consisting of C 1-18  alkyl, C 2-18  alkenyl, —R*YR″, —YR″, and H; 
     each R″ is independently selected from the group consisting of C 3-14  alkyl and C 3-14  alkenyl; 
     each R* is independently selected from the group consisting of C 1-12  alkyl and C 1-12  alkenyl; 
     each Y is independently a C 3-6  carbocycle; 
     each X is independently selected from the group consisting of F, Cl, Br, and I; and 
     m is selected from 5, 6, 7, 8, 9, 10, 11, 12, and 13, 
     or salts or isomers thereof. 
     In some embodiments, another subset of compounds of Formula (I) includes those in which 
     R 1  is selected from the group consisting of C 5-30  alkyl, C 5-20  alkenyl, —R*YR″, —YR″, and —R″M′R′; 
     R 2  and R 3  are independently selected from the group consisting of C 1-14  alkyl, C 2-14  alkenyl, —R*YR″, —YR″, and —R*OR″, or R 2  and R 3 , together with the atom to which they are attached, form a heterocycle or carbocycle; 
     R 4  is selected from the group consisting of —(CH 2 ) n Q, —(CH 2 ) n CHQR, —CHQR, and —CQ(R) 2 , where Q is —N(R) 2 , and n is selected from 1, 2, 3, 4, and 5; 
     each R 5  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R 6  is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     M and M′ are independently selected from —C(O)O—, —OC(O)—, —C(O)N(R′)—, —N(R′)C(O)—, —C(O)—, —C(S)—, —C(S)S—, —SC(S)—, —CH(OH)—, —P(O)(OR′)O—, —S(O) 2 —, —S—S—, an aryl group, and a heteroaryl group; 
     R 7  is selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R is independently selected from the group consisting of C 1-3  alkyl, C 2-3  alkenyl, and H; 
     each R′ is independently selected from the group consisting of C 1-18  alkyl, C 2-18  alkenyl, —R*YR″, —YR″, and H; 
     each R″ is independently selected from the group consisting of C 3-14  alkyl and C 3-14  alkenyl; 
     each R* is independently selected from the group consisting of C 1-12  alkyl and C 1-12  alkenyl; 
     each Y is independently a C 3-6  carbocycle; 
     each X is independently selected from the group consisting of F, Cl, Br, and I; and 
     m is selected from 5, 6, 7, 8, 9, 10, 11, 12, and 13, 
     or salts or isomers thereof. 
     In some embodiments, a subset of compounds of Formula (I) includes those of Formula (IA): 
     
       
         
         
             
             
         
       
     
     or a salt or isomer thereof, wherein 1 is selected from 1, 2, 3, 4, and 5; m is selected from 5, 6, 7, 8, and 9; M 1  is a bond or M′; R 4  is unsubstituted C 1-3  alkyl, or —(CH 2 ) n Q, in which Q is OH, —NHC(S)N(R) 2 , —NHC(O)N(R) 2 , —N(R)C(O)R, —N(R)S(O) 2 R, —N(R)R 8 , —NHC(═NR 9 )N(R) 2 , —NHC(═CHR 9 )N(R) 2 , —OC(O)N(R) 2 , —N(R)C(O)OR, heteroaryl or heterocycloalkyl; M and M′ are independently selected from —C(O)O—, —OC(O)—, —C(O)N(R′)—, —P(O)(OR′)O—, —S—S—, an aryl group, and a heteroaryl group; and R 2  and R 3  are independently selected from the group consisting of H, C 1-14  alkyl, and C 2-14  alkenyl. 
     In some embodiments, a subset of compounds of Formula (I) includes those of Formula (II): 
     
       
         
         
             
             
         
       
     
     or a salt or isomer thereof, wherein 1 is selected from 1, 2, 3, 4, and 5; M 1  is a bond or M′; R 4  is unsubstituted C 1-3  alkyl, or —(CH 2 ) n Q, in which n is 2, 3, or 4, and Q is OH, —NHC(S)N(R) 2 , —NHC(O)N(R) 2 , —N(R)C(O)R, —N(R)S(O) 2 R, —N(R)R 8 , —NHC(═NR 9 )N(R) 2 , —NHC(═CHR 9 )N(R) 2 , —OC(O)N(R) 2 , —N(R)C(O)OR, heteroaryl or heterocycloalkyl; M and M′ are independently selected from —C(O)O—, —OC(O)—, —C(O)N(R′)—, —P(O)(OR′)O—, —S—S—, an aryl group, and a heteroaryl group; and R 2  and R 3  are independently selected from the group consisting of H, C 1-14  alkyl, and C 2-14  alkenyl. 
     In some embodiments, a subset of compounds of Formula (I) includes those of Formula (IIa), (IIb), (IIc), or (IIe): 
     
       
         
         
             
             
         
       
     
     or a salt or isomer thereof, wherein R 4  is as described herein. 
     In some embodiments, a subset of compounds of Formula (I) includes those of Formula (IId): 
     
       
         
         
             
             
         
       
     
     or a salt or isomer thereof, wherein n is 2, 3, or 4; and m, R′, R″, and R 2  through R 6  are as described herein. For example, each of R 2  and R 3  may be independently selected from the group consisting of C 5-14  alkyl and C 5-14  alkenyl. 
     In some embodiments, an ionizable cationic lipid of the disclosure comprises a compound having structure: 
     
       
         
         
             
             
         
       
     
     In some embodiments, an ionizable cationic lipid of the disclosure comprises a compound having structure: 
     
       
         
         
             
             
         
       
     
     In some embodiments, a non-cationic lipid of the disclosure comprises 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (DLPC), 1,2-dimyristoyl-sn-gly cero-phosphocholine (DMPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-diundecanoyl-sn-glycero-phosphocholine (DUPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-di-O-octadecenyl-sn-glycero-3-phosphocholine (18:0 Diether PC), 1-oleoyl-2 cholesterylhemisuccinoyl-sn-glycero-3-phosphocholine (OChemsPC), 1-hexadecyl-sn-glycero-3-phosphocholine (C16 Lyso PC), 1,2-dilinolenoyl-sn-glycero-3-phosphocholine, 1,2-diarachidonoyl-sn-glycero-3-phosphocholine, 1,2-didocosahexaenoyl-sn-glycero-3-phosphocholine, 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine (ME 16.0 PE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine, 1,2-dilinoleoyl-sn-glycero-3-phosphoethanolamine, 1,2-dilinolenoyl-sn-glycero-3-phosphoethanolamine, 1,2-diarachidonoyl-sn-glycero-3-phosphoethanolamine, 1,2-didocosahexaenoyl-sn-glycero-3-phosphoethanolamine, 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DOPG), sphingomyelin, and mixtures thereof. 
     In some embodiments, a PEG modified lipid of the disclosure comprises a PEG-modified phosphatidylethanolamine, a PEG-modified phosphatidic acid, a PEG-modified ceramide, a PEG-modified dialkylamine, a PEG-modified diacylglycerol, a PEG-modified dialkylglycerol, and mixtures thereof. In some embodiments, the PEG-modified lipid is PEG-DMG, PEG-c-DOMG (also referred to as PEG-DOMG), PEG-DSG and/or PEG-DPG. 
     In some embodiments, a sterol of the disclosure comprises cholesterol, fecosterol, sitosterol, ergosterol, campesterol, stigmasterol, brassicasterol, tomatidine, ursolic acid, alpha-tocopherol, and mixtures thereof. 
     In some embodiments, a LNP of the disclosure comprises an ionizable cationic lipid of Compound 1, wherein the non-cationic lipid is DSPC, the structural lipid that is cholesterol, and the PEG lipid is PEG-DMG. 
     In some embodiments, a LNP of the disclosure comprises an N:P ratio of from about 2:1 to about 30:1. 
     In some embodiments, a LNP of the disclosure comprises an N:P ratio of about 6:1. 
     In some embodiments, a LNP of the disclosure comprises an N:P ratio of about 3:1. 
     In some embodiments, a LNP of the disclosure comprises a wt/wt ratio of the ionizable cationic lipid component to the RNA of from about 10:1 to about 100:1. 
     In some embodiments, a LNP of the disclosure comprises a wt/wt ratio of the ionizable cationic lipid component to the RNA of about 20:1. 
     In some embodiments, a LNP of the disclosure comprises a wt/wt ratio of the ionizable cationic lipid component to the RNA of about 10:1. 
     In some embodiments, a LNP of the disclosure has a mean diameter from about 50 nm to about 150 nm. 
     In some embodiments, a LNP of the disclosure has a mean diameter from about 70 nm to about 120 nm. 
     Multivalent Vaccines 
     The EBV vaccines, as provided herein, may include an RNA (e.g. mRNA) or multiple RNAs encoding two or more antigens of the same or different EBV species. In some embodiments, an EBV vaccine includes an RNA or multiple RNAs encoding two or more antigens selected from gp350, gH, gL, gB, gp42, LMP1, LMP2, EBNA1 and EBNA3 antigens. In some embodiments, the RNA (at least one RNA) of an EBV vaccine may encode 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or more antigens. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen and a gH antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen and a gL antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen and a gB antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen and a gp42 antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen and a LMP (e.g., LMP1 and/or LMP2) antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gH antigen and a gL antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gH antigen and a gB antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gH antigen and a gp42 antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gH antigen and a LMP (e.g., LMP1 and/or LMP2) antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gH antigen and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gL antigen and a gB antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gL antigen and a gp42 antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gL antigen and a LMP (e.g., LMP1 and/or LMP2) antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gL antigen and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gB antigen and a gp42 antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gB antigen and a LMP (e.g., LMP1 and/or LMP2) antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gB antigen and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gp42 antigen and a LMP (e.g., LMP1 and/or LMP2) antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, gp42 antigen and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp350 antigen, a LMP (e.g., LMP1 and/or LMP2) antigen, and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a gH antigen and a gL antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gH antigen and a gB antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gH antigen and a gp42 antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gH antigen and a LMP (e.g., LMP1 and/or LMP2) antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gH antigen and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a gL antigen and a gB antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gL antigen and a gp42 antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gL antigen and a LMP (e.g., LMP1 and/or LMP2) antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gL antigen and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a gB antigen and a gp42 antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gB antigen and a LMP (e.g., LMP1 and/or LMP2) antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gB antigen and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp42 antigen and a LMP (e.g., LMP1 and/or LMP2) antigen. In some embodiments, an EBV vaccine comprises at least one RNA encoding a gp42 antigen and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, an EBV vaccine comprises at least one RNA encoding a LMP (e.g., LMP1 and/or LMP2) antigen and an EBNA (e.g., EBNA1 and/or EBNA3) antigen. 
     In some embodiments, two or more different RNA (e.g., mRNA) encoding antigens may be formulated in the same lipid nanoparticle. In other embodiments, two or more different RNA encoding antigens may be formulated in separate lipid nanoparticles (each RNA formulated in a single lipid nanoparticle). The lipid nanoparticles may then be combined and administered as a single vaccine composition (e.g., comprising multiple RNA encoding multiple antigens) or may be administered separately. 
     Combination Vaccines 
     The EBV vaccines, as provided herein, may include an RNA or multiple RNAs encoding two or more antigens of the same or different EBV strains. Also provided herein are combination vaccines that include RNA encoding one or more EBV antigen(s) and one or more antigen(s) of a different organisms (e.g., bacterial and/or viral organism). Thus, the vaccines of the present disclosure may be combination vaccines that target one or more antigens of the same strain/species, or one or more antigens of different strains/species, e.g., antigens which induce immunity to organisms which are found in the same geographic areas where the risk of EBV infection is high or organisms to which an individual is likely to be exposed to when exposed to EBV. 
     Dosing/Administration 
     Provided herein are compositions (e.g., pharmaceutical compositions), methods, kits and reagents for prevention and/or treatment of EBV in humans and other mammals. EBV RNA vaccines can be used as therapeutic or prophylactic agents. In some aspects, the RNA vaccines of the disclosure are used to provide prophylactic protection from EBV. In some aspects, the RNA vaccines of the disclosure are used to treat an EBV infection. In some embodiments, the EBV vaccines of the present disclosure are used in the priming of immune effector cells, for example, to activate peripheral blood mononuclear cells (PBMCs) ex vivo, which are then infused (re-infused) into a subject. 
     A subject may be any mammal, including non-human primate and human subjects. Typically, a subject is a human subject. 
     In some embodiments, the EBV vaccines are administered to a subject (e.g., a mammalian subject, such as a human subject) in an effective amount to induce an antigen-specific immune response. The RNA encoding the EBV antigen is expressed and translated in vivo to produce the antigen, which then stimulates an immune response in the subject. 
     Prophylactic protection from EBV can be achieved following administration of an EBV RNA vaccine of the present disclosure. Vaccines can be administered once, twice, three times, four times or more but it is likely sufficient to administer the vaccine once (optionally followed by a single booster). It is possible, although less desirable, to administer the vaccine to an infected individual to achieve a therapeutic response. Dosing may need to be adjusted accordingly. 
     A method of eliciting an immune response in a subject against EBV is provided in aspects of the present disclosure. The method involves administering to the subject an EBV RNA vaccine comprising at least one RNA (e.g., mRNA) having an open reading frame encoding at least one EBV antigen, thereby inducing in the subject an immune response specific to EBV antigen, wherein anti-antigen antibody titer in the subject is increased following vaccination relative to anti-antigen antibody titer in a subject vaccinated with a prophylactically effective dose of a traditional vaccine against the EBV. An “anti-antigen antibody” is a serum antibody the binds specifically to the antigen. 
     A prophylactically effective dose is an effective dose that prevents infection with the virus at a clinically acceptable level. In some embodiments, the effective dose is a dose listed in a package insert for the vaccine. A traditional vaccine, as used herein, refers to a vaccine other than the mRNA vaccines of the present disclosure. For instance, a traditional vaccine includes, but is not limited, to live microorganism vaccines, killed microorganism vaccines, subunit vaccines, protein antigen vaccines, DNA vaccines, virus like particle (VLP) vaccines, etc. In exemplary embodiments, a traditional vaccine is a vaccine that has achieved regulatory approval and/or is registered by a national drug regulatory body, for example the Food and Drug Administration (FDA) in the United States or the European Medicines Agency (EMA). 
     In some embodiments, the anti-antigen antibody titer in the subject is increased 1 log to 10 log following vaccination relative to anti-antigen antibody titer in a subject vaccinated with a prophylactically effective dose of a traditional vaccine against the EBV or an unvaccinated subject. In some embodiments, the anti-antigen antibody titer in the subject is increased 1 log, 2 log, 3 log, 4 log, 5 log, or 10 log following vaccination relative to anti-antigen antibody titer in a subject vaccinated with a prophylactically effective dose of a traditional vaccine against the EBV or an unvaccinated subject. 
     A method of eliciting an immune response in a subject against an EBV is provided in other aspects of the disclosure. The method involves administering to the subject an EBV RNA vaccine comprising at least one RNA polynucleotide having an open reading frame encoding at least one EBV antigen, thereby inducing in the subject an immune response specific to EBV antigen, wherein the immune response in the subject is equivalent to an immune response in a subject vaccinated with a traditional vaccine against the EBV at 2 times to 100 times the dosage level relative to the RNA vaccine. 
     In some embodiments, the immune response in the subject is equivalent to an immune response in a subject vaccinated with a traditional vaccine at twice the dosage level relative to the EBV RNA vaccine. In some embodiments, the immune response in the subject is equivalent to an immune response in a subject vaccinated with a traditional vaccine at three times the dosage level relative to the EBV RNA vaccine. In some embodiments, the immune response in the subject is equivalent to an immune response in a subject vaccinated with a traditional vaccine at 4 times, 5 times, 10 times, 50 times, or 100 times the dosage level relative to the EBV RNA vaccine. In some embodiments, the immune response in the subject is equivalent to an immune response in a subject vaccinated with a traditional vaccine at 10 times to 1000 times the dosage level relative to the EBV RNA vaccine. In some embodiments, the immune response in the subject is equivalent to an immune response in a subject vaccinated with a traditional vaccine at 100 times to 1000 times the dosage level relative to the EBV RNA vaccine. 
     In other embodiments, the immune response is assessed by determining [protein] antibody titer in the subject. In other embodiments, the ability of serum or antibody from an immunized subject is tested for its ability to neutralize viral uptake or reduce EBV transformation of human B lymphocytes. In other embodiments, the ability to promote a robust T cell response(s) is measured using art recognized techniques. 
     Other aspects the disclosure provide methods of eliciting an immune response in a subject against an EBV by administering to the subject an EBV RNA vaccine comprising at least one RNA polynucleotide having an open reading frame encoding at least one EBV antigen, thereby inducing in the subject an immune response specific to EBV antigen, wherein the immune response in the subject is induced 2 days to 10 weeks earlier relative to an immune response induced in a subject vaccinated with a prophylactically effective dose of a traditional vaccine against the EBV. In some embodiments, the immune response in the subject is induced in a subject vaccinated with a prophylactically effective dose of a traditional vaccine at 2 times to 100 times the dosage level relative to the RNA vaccine. 
     In some embodiments, the immune response in the subject is induced 2 days, 3 days, 1 week, 2 weeks, 3 weeks, 5 weeks, or 10 weeks earlier relative to an immune response induced in a subject vaccinated with a prophylactically effective dose of a traditional vaccine. 
     Also provided herein are methods of eliciting an immune response in a subject against an EBV by administering to the subject an EBV RNA vaccine having an open reading frame encoding a first antigen, wherein the RNA polynucleotide does not include a stabilization element, and wherein an adjuvant is not co-formulated or co-administered with the vaccine. 
     EBV RNA (e.g., mRNA) vaccines may be administered by any route which results in a therapeutically effective outcome. These include, but are not limited, to intradermal, intramuscular, intranasal, and/or subcutaneous administration. The present disclosure provides methods comprising administering RNA vaccines to a subject in need thereof. The exact amount required will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the disease, the particular composition, its mode of administration, its mode of activity, and the like. EBV RNA (e.g., mRNA) vaccines compositions are typically formulated in dosage unit form for ease of administration and uniformity of dosage. It will be understood, however, that the total daily usage of EBV RNA (e.g., mRNA)vaccines compositions may be decided by the attending physician within the scope of sound medical judgment. The specific therapeutically effective, prophylactically effective, or appropriate imaging dose level for any particular patient will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed; and like factors well known in the medical arts. 
     The effective amount of an EBV vaccine, as provided herein, may be as low as 20 μg, administered for example as a single dose or as two 10 μg doses. In some embodiments, the effective amount is a total dose of 20 μg-200 μg. For example, the effective amount may be a total dose of 20 μg, 25 μg, 30 μg, 35 μg, 40 μg, 45 μg, 50 μg, 55 μg, 60 μg, 65 μg, 70 μg, 75 μg, 80 μg, 85 μg, 90 μg, 95 μg, 100 μg, 110 μg, 120 μg, 130 μg, 140 μg, 150 μg, 160 μg, 170 μg, 180 μg, 190 μg or 200 μg. In some embodiments, the effective amount is a total dose of 25 μg-200 μg. In some embodiments, the effective amount is a total dose of 50 μg-200 μg. 
     In some embodiments, EBV RNA (e.g., mRNA) vaccines compositions may be administered at dosage levels sufficient to deliver 0.0001 mg/kg to 100 mg/kg, 0.001 mg/kg to 0.05 mg/kg, 0.005 mg/kg to 0.05 mg/kg, 0.001 mg/kg to 0.005 mg/kg, 0.05 mg/kg to 0.5 mg/kg, 0.01 mg/kg to 50 mg/kg, 0.1 mg/kg to 40 mg/kg, 0.5 mg/kg to 30 mg/kg, 0.01 mg/kg to 10 mg/kg, 0.1 mg/kg to 10 mg/kg, or 1 mg/kg to 25 mg/kg, of subject body weight per day, one or more times a day, per week, per month, etc. to obtain the desired therapeutic, diagnostic, prophylactic, or imaging effect (see e.g., the range of unit doses described in International Publication No. WO2013078199, herein incorporated by reference in its entirety). The desired dosage may be delivered three times a day, two times a day, once a day, every other day, every third day, every week, every two weeks, every three weeks, every four weeks, every 2 months, every three months, every 6 months, etc. In certain embodiments, the desired dosage may be delivered using multiple administrations (e.g., two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, or more administrations). When multiple administrations are employed, split dosing regimens such as those described herein may be used. In exemplary embodiments, EBV RNA (e.g., mRNA) vaccines compositions may be administered at dosage levels sufficient to deliver 0.0005 mg/kg to 0.01 mg/kg, e.g., about 0.0005 mg/kg to about 0.0075 mg/kg, e.g., about 0.0005 mg/kg, about 0.001 mg/kg, about 0.002 mg/kg, about 0.003 mg/kg, about 0.004 mg/kg or about 0.005 mg/kg. 
     In some embodiments, EBV RNA (e.g., mRNA) vaccine compositions may be administered once or twice (or more) at dosage levels sufficient to deliver 0.025 mg/kg to 0.250 mg/kg, 0.025 mg/kg to 0.500 mg/kg, 0.025 mg/kg to 0.750 mg/kg, or 0.025 mg/kg to 1.0 mg/kg. 
     In some embodiments, EBV RNA (e.g., mRNA) vaccine compositions may be administered twice (e.g., Day 0 and Day 7, Day 0 and Day 14, Day 0 and Day 21, Day 0 and Day 28, Day 0 and Day 60, Day 0 and Day 90, Day 0 and Day 120, Day 0 and Day 150, Day 0 and Day 180, Day 0 and 3 months later, Day 0 and 6 months later, Day 0 and 9 months later, Day 0 and 12 months later, Day 0 and 18 months later, Day 0 and 2 years later, Day 0 and 5 years later, or Day 0 and 10 years later) at a total dose of or at dosage levels sufficient to deliver a total dose of 0.0100 mg, 0.025 mg, 0.050 mg, 0.075 mg, 0.100 mg, 0.125 mg, 0.150 mg, 0.175 mg, 0.200 mg, 0.225 mg, 0.250 mg, 0.275 mg, 0.300 mg, 0.325 mg, 0.350 mg, 0.375 mg, 0.400 mg, 0.425 mg, 0.450 mg, 0.475 mg, 0.500 mg, 0.525 mg, 0.550 mg, 0.575 mg, 0.600 mg, 0.625 mg, 0.650 mg, 0.675 mg, 0.700 mg, 0.725 mg, 0.750 mg, 0.775 mg, 0.800 mg, 0.825 mg, 0.850 mg, 0.875 mg, 0.900 mg, 0.925 mg, 0.950 mg, 0.975 mg, or 1.0 mg. Higher and lower dosages and frequency of administration are encompassed by the present disclosure. For example, an EBV RNA (e.g., mRNA) vaccine composition may be administered three or four times. 
     In some embodiments, EBV RNA (e.g., mRNA) vaccine compositions may be administered twice (e.g., Day 0 and Day 7, Day 0 and Day 14, Day 0 and Day 21, Day 0 and Day 28, Day 0 and Day 60, Day 0 and Day 90, Day 0 and Day 120, Day 0 and Day 150, Day 0 and Day 180, Day 0 and 3 months later, Day 0 and 6 months later, Day 0 and 9 months later, Day 0 and 12 months later, Day 0 and 18 months later, Day 0 and 2 years later, Day 0 and 5 years later, or Day 0 and 10 years later) at a total dose of or at dosage levels sufficient to deliver a total dose of 0.010 mg, 0.025 mg, 0.100 mg or 0.400 mg. 
     In some embodiments, the EBV RNA (e.g., mRNA) vaccine for use in a method of vaccinating a subject is administered the subject a single dosage of between 10 μg/kg and 400 μg/kg of the nucleic acid vaccine in an effective amount to vaccinate the subject. In some embodiments, the RNA vaccine for use in a method of vaccinating a subject is administered the subject a single dosage of between 10 μg and 400 μg of the nucleic acid vaccine in an effective amount to vaccinate the subject. In some embodiments, an EBV RNA (e.g., mRNA) vaccine for use in a method of vaccinating a subject is administered to the subject as a single dosage of 25-1000 μg (e.g., a single dosage of mRNA encoding an EBV antigen). In some embodiments, an EBV RNA vaccine is administered to the subject as a single dosage of 25, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950 or 1000 μg. For example, an EBV RNA vaccine may be administered to a subject as a single dose of 25-100, 25-500, 50-100, 50-500, 50-1000, 100-500, 100-1000, 250-500, 250-1000, or 500-1000 μg. In some embodiments, an EBV RNA (e.g., mRNA) vaccine for use in a method of vaccinating a subject is administered to the subject as two dosages, the combination of which equals 25-1000 μg of the EBV RNA (e.g., mRNA) vaccine. 
     AN EBV RNA (e.g., mRNA) vaccine pharmaceutical composition described herein can be formulated into a dosage form described herein, such as an intranasal, intratracheal, or injectable (e.g., intravenous, intraocular, intravitreal, intramuscular, intradermal, intracardiac, intraperitoneal, and subcutaneous). 
     Vaccine Efficacy 
     Some aspects of the present disclosure provide formulations of the EBV RNA (e.g., mRNA) vaccine, wherein the EBV RNA vaccine is formulated in an effective amount to produce an antigen specific immune response in a subject (e.g., production of antibodies specific to an anti-EBV antigen). “An effective amount” is a dose of an EBV RNA (e.g., mRNA) vaccine effective to produce an antigen-specific immune response. Also provided herein are methods of inducing an antigen-specific immune response in a subject. 
     As used herein, an immune response to a vaccine or LNP of the present invention is the development in a subject of a humoral and/or a cellular immune response to a (one or more) EBV protein(s) present in the vaccine. For purposes of the present invention, a “humoral” immune response refers to an immune response mediated by antibody molecules, including, e.g., secretory (IgA) or IgG molecules, while a “cellular” immune response is one mediated by T-lymphocytes (e.g., CD4+ helper and/or CD8+ T cells (e.g., CTLs) and/or other white blood cells. One important aspect of cellular immunity involves an antigen-specific response by cytolytic T-cells (CTLs). CTLs have specificity for peptide antigens that are presented in association with proteins encoded by the major histocompatibility complex (MHC) and expressed on the surfaces of cells. CTLs help induce and promote the destruction of intracellular microbes or the lysis of cells infected with such microbes. Another aspect of cellular immunity involves and antigen-specific response by helper T-cells. Helper T-cells act to help stimulate the function, and focus the activity nonspecific effector cells against cells displaying peptide antigens in association with MHC molecules on their surface. A cellular immune response also leads to the production of cytokines, chemokines, and other such molecules produced by activated T-cells and/or other white blood cells including those derived from CD4+ and CD8+ T-cells. 
     In some embodiments, the antigen-specific immune response is characterized by measuring an anti-EBV antigen antibody titer produced in a subject administered an EBV RNA (e.g., mRNA) vaccine as provided herein. An antibody titer is a measurement of the amount of antibodies within a subject, for example, antibodies that are specific to a particular antigen (e.g., an anti-EBV antigen) or epitope of an antigen. Antibody titer is typically expressed as the inverse of the greatest dilution that provides a positive result. Enzyme-linked immunosorbent assay (ELISA) is a common assay for determining antibody titers, for example. 
     In some embodiments, an antibody titer is used to assess whether a subject has had an infection or to determine whether immunizations are required. In some embodiments, an antibody titer is used to determine the strength of an autoimmune response, to determine whether a booster immunization is needed, to determine whether a previous vaccine was effective, and to identify any recent or prior infections. In accordance with the present disclosure, an antibody titer may be used to determine the strength of an immune response induced in a subject by the EBV RNA (e.g., mRNA) vaccine. 
     In some embodiments, an anti-EBV antigen antibody titer produced in a subject is increased by at least 1 log relative to a control. For example, anti-EBV antigen antibody titer produced in a subject may be increased by at least 1.5, at least 2, at least 2.5, or at least 3 log relative to a control. In some embodiments, the anti-EBV antigen antibody titer produced in the subject is increased by 1, 1.5, 2, 2.5 or 3 log relative to a control. In some embodiments, the anti-EBV antigen antibody titer produced in the subject is increased by 1-3 log relative to a control. For example, the anti-EBV antigen antibody titer produced in a subject may be increased by 1-1.5, 1-2, 1-2.5, 1-3, 1.5-2, 1.5-2.5, 1.5-3, 2-2.5, 2-3, or 2.5-3 log relative to a control. 
     In some embodiments, the anti-EBV antigen antibody titer produced in a subject is increased at least 2 times relative to a control. For example, the anti-EBV antigen antibody titer produced in a subject may be increased at least 3 times, at least 4 times, at least 5 times, at least 6 times, at least 7 times, at least 8 times, at least 9 times, or at least 10 times relative to a control. In some embodiments, the anti-EBV antigen antibody titer produced in the subject is increased 2, 3, 4, 5, 6, 7, 8, 9, or 10 times relative to a control. In some embodiments, the anti-EBV antigen antibody titer produced in a subject is increased 2-10 times relative to a control. For example, the anti-EBV antigen antibody titer produced in a subject may be increased 2-10, 2-9, 2-8, 2-7, 2-6, 2-5, 2-4, 2-3, 3-10, 3-9, 3-8, 3-7, 3-6, 3-5, 3-4, 4-10, 4-9, 4-8, 4-7, 4-6, 4-5, 5-10, 5-9, 5-8, 5-7, 5-6, 6-10, 6-9, 6-8, 6-7, 7-10, 7-9, 7-8, 8-10, 8-9, or 9-10 times relative to a control. 
     A control, in some embodiments, is the anti-EBV antigen antibody titer produced in a subject who has not been administered an EBV RNA (e.g., mRNA) vaccine. In some embodiments, a control is an anti-EBV antigen antibody titer produced in a subject administered a recombinant or purified EBV protein vaccine. Recombinant protein vaccines typically include protein antigens that either have been produced in a heterologous expression system (e.g., bacteria or yeast) or purified from large amounts of the pathogenic organism. 
     In some embodiments, the ability of an EBV vaccine to be effective is measured in a murine model. For example, the EBV vaccines may be administered to a murine model and the murine model assayed for induction of neutralizing antibody titers. Viral challenge studies may also be used to assess the efficacy of a vaccine of the present disclosure. For example, the EBV vaccines may be administered to a murine model, the murine model challenged with EBV, and the murine model assayed for survival and/or immune response (e.g., neutralizing antibody response, T cell response (e.g., cytokine response)). 
     In some embodiments, an effective amount of an EBV RNA (e.g., mRNA) vaccine is a dose that is reduced compared to the standard of care dose of a recombinant EBV protein vaccine. A “standard of care,” as provided herein, refers to a medical or psychological treatment guideline and can be general or specific. “Standard of care” specifies appropriate treatment based on scientific evidence and collaboration between medical professionals involved in the treatment of a given condition. It is the diagnostic and treatment process that a physician/clinician should follow for a certain type of patient, illness or clinical circumstance. A “standard of care dose,” as provided herein, refers to the dose of a recombinant or purified EBV protein vaccine, or a live attenuated or inactivated EBV vaccine, or an EBV VLP vaccine, that a physician/clinician or other medical professional would administer to a subject to treat or prevent EBV, or an EBV-related condition, while following the standard of care guideline for treating or preventing EBV, or an EBV-related condition. 
     In some embodiments, the anti-EBV antigen antibody titer produced in a subject administered an effective amount of an EBV RNA vaccine is equivalent to an anti-EBV antigen antibody titer produced in a control subject administered a standard of care dose of a recombinant or purified EBV protein vaccine, or a live attenuated or inactivated EBV vaccine, or an EBV VLP vaccine. 
     In some embodiments, an effective amount of an EBV RNA (e.g., mRNA) vaccine is a dose equivalent to an at least 2-fold reduction in a standard of care dose of a recombinant or purified EBV protein vaccine. For example, an effective amount of an EBV RNA vaccine may be a dose equivalent to an at least 3-fold, at least 4-fold, at least 5-fold, at least 6-fold, at least 7-fold, at least 8-fold, at least 9-fold, or at least 10-fold reduction in a standard of care dose of a recombinant or purified EBV protein vaccine. In some embodiments, an effective amount of an EBV RNA vaccine is a dose equivalent to an at least at least 100-fold, at least 500-fold, or at least 1000-fold reduction in a standard of care dose of a recombinant or purified EBV protein vaccine. In some embodiments, an effective amount of an EBV RNA vaccine is a dose equivalent to a 2-, 3-, 4-, 5-, 6-, 7-, 8-, 9-, 10-, 20-, 50-, 100-, 250-, 500-, or 1000-fold reduction in a standard of care dose of a recombinant or purified EBV protein vaccine. In some embodiments, the anti-EBV antigen antibody titer produced in a subject administered an effective amount of an EBV RNA vaccine is equivalent to an anti-EBV antigen antibody titer produced in a control subject administered the standard of care dose of a recombinant or protein EBV protein vaccine, or a live attenuated or inactivated EBV vaccine, or an EBV VLP vaccine. In some embodiments, an effective amount of an EBV RNA (e.g., mRNA) vaccine is a dose equivalent to a 2-fold to 1000-fold (e.g., 2-fold to 100-fold, 10-fold to 1000-fold) reduction in the standard of care dose of a recombinant or purified EBV protein vaccine, wherein the anti-EBV antigen antibody titer produced in the subject is equivalent to an anti-EBV antigen antibody titer produced in a control subject administered the standard of care dose of a recombinant or purified EBV protein vaccine, or a live attenuated or inactivated EBV vaccine, or an EBV VLP vaccine. 
     In some embodiments, the effective amount of an EBV RNA (e.g., mRNA) vaccine is a dose equivalent to a 2 to 1000-, 2 to 900-, 2 to 800-, 2 to 700-, 2 to 600-, 2 to 500-, 2 to 400-, 2 to 300-, 2 to 200-, 2 to 100-, 2 to 90-, 2 to 80-, 2 to 70-, 2 to 60-, 2 to 50-, 2 to 40-, 2 to 30-, 2 to 20-, 2 to 10-, 2 to 9-, 2 to 8-, 2 to 7-, 2 to 6-, 2 to 5-, 2 to 4-, 2 to 3-, 3 to 1000-, 3 to 900-, 3 to 800-, 3 to 700-, 3 to 600-, 3 to 500-, 3 to 400-, 3 to 3 to 00-, 3 to 200-, 3 to 100-, 3 to 90-, 3 to 80-, 3 to 70-, 3 to 60-, 3 to 50-, 3 to 40-, 3 to 30-, 3 to 20-, 3 to 10-, 3 to 9-, 3 to 8-, 3 to 7-, 3 to 6-, 3 to 5-, 3 to 4-, 4 to 1000-, 4 to 900-, 4 to 800-, 4 to 700-, 4 to 600-, 4 to 500-, 4 to 400-, 4 to 300-, 4 to 200-, 4 to 100-, 4 to 90-, 4 to 80-, 4 to 70-, 4 to 60-, 4 to 50-, 4 to 40-, 4 to 30-, 4 to 20-, 4 to 10-, 4 to 9-, 4 to 8-, 4 to 7-, 4 to 6-, 4 to 5-, 4 to 4-, 5 to 1000-, 5 to 900-, 5 to 800-, 5 to 700-, 5 to 600-, 5 to 500-, 5 to 400-, 5 to 300-, 5 to 200-, 5 to 100-, 5 to 90-, 5 to 80-, 5 to 70-, 5 to 60-, 5 to 50-, 5 to 40-, 5 to 30-, 5 to 20-, 5 to 10-, 5 to 9-, 5 to 8, 5 to 7-, 5 to 6-, 6 to 1000-, 6 to 900-, 6 to 800-, 6 to 700-, 6 to 600-, 6 to 500-, 6 to 400-, 6 to 300-, 6 to 200-, 6 to 100-, 6 to 90-, 6 to 80-, 6 to 70-, 6 to 60-, 6 to 50-, 6 to 40-, 6 to 30-, 6 to 20-, 6 to 10-, 6 to 9-, 6 to 8-, 6 to 7-, 7 to 1000-, 7 to 900-, 7 to 800-, 7 to 700-, 7 to 600-, 7 to 500-, 7 to 400-, 7 to 300-, 7 to 200-, 7 to 100-, 7 to 90-, 7 to 80-, 7 to 70-, 7 to 60-, 7 to 50-, 7 to 40-, 7 to 30-, 7 to 20-, 7 to 10-, 7 to 9-, 7 to 8-, 8 to 1000-, 8 to 900-, 8 to 800-, 8 to 700-, 8 to 600-, 8 to 500-, 8 to 400-, 8 to 300-, 8 to 200-, 8 to 100-, 8 to 90-, 8 to 80-, 8 to 70-, 8 to 60-, 8 to 50-, 8 to 40-, 8 to 30-, 8 to 20-, 8 to 10-, 8 to 9-, 9 to 1000-, 9 to 900-, 9 to 800-, 9 to 700-, 9 to 600-, 9 to 500-, 9 to 400-, 9 to 300-, 9 to 200-, 9 to 100-, 9 to 90-, 9 to 80-, 9 to 70-, 9 to 60-, 9 to 50-, 9 to 40-, 9 to 30-, 9 to 20-, 9 to 10-, 10 to 1000-, 10 to 900-, 10 to 800-, 10 to 700-, 10 to 600-, 10 to 500-, 10 to 400-, 10 to 300-, 10 to 200-, 10 to 100-, 10 to 90-, 10 to 80-, 10 to 70-, 10 to 60-, 10 to 50-, 10 to 40-, 10 to 30-, 10 to 20-, 20 to 1000-, 20 to 900-, 20 to 800-, 20 to 700-, 20 to 600-, 20 to 500-, 20 to 400-, 20 to 300-, 20 to 200-, 20 to 100-, 20 to 90-, 20 to 80-, 20 to 70-, 20 to 60-, 20 to 50-, 20 to 40-, 20 to 30-, 30 to 1000-, 30 to 900-, 30 to 800-, 30 to 700-, 30 to 600-, 30 to 500-, 30 to 400-, 30 to 300-, 30 to 200-, 30 to 100-, 30 to 90-, 30 to 80-, 30 to 70-, 30 to 60-, 30 to 50-, 30 to 40-, 40 to 1000-, 40 to 900-, 40 to 800-, 40 to 700-, 40 to 600-, 40 to 500-, 40 to 400-, 40 to 300-, 40 to 200-, 40 to 100-, 40 to 90-, 40 to 80-, 40 to 70-, 40 to 60-, 40 to 50-, 50 to 1000-, 50 to 900-, 50 to 800-, 50 to 700-, 50 to 600-, 50 to 500-, 50 to 400-, 50 to 300-, 50 to 200-, 50 to 100-, 50 to 90-, 50 to 80-, 50 to 70-, 50 to 60-, 60 to 1000-, 60 to 900-, 60 to 800-, 60 to 700-, 60 to 600-, 60 to 500-, 60 to 400-, 60 to 300-, 60 to 200-, 60 to 100-, 60 to 90-, 60 to 80-, 60 to 70-, 70 to 1000-, 70 to 900-, 70 to 800-, 70 to 700-, 70 to 600-, 70 to 500-, 70 to 400-, 70 to 300-, 70 to 200-, 70 to 100-, 70 to 90-, 70 to 80-, 80 to 1000-, 80 to 900-, 80 to 800-, 80 to 700-, 80 to 600-, 80 to 500-, 80 to 400-, 80 to 300-, 80 to 200-, 80 to 100-, 80 to 90-, 90 to 1000-, 90 to 900-, 90 to 800-, 90 to 700-, 90 to 600-, 90 to 500-, 90 to 400-, 90 to 300-, 90 to 200-, 90 to 100-, 100 to 1000-, 100 to 900-, 100 to 800-, 100 to 700-, 100 to 600-, 100 to 500-, 100 to 400-, 100 to 300-, 100 to 200-, 200 to 1000-, 200 to 900-, 200 to 800-, 200 to 700-, 200 to 600-, 200 to 500-, 200 to 400-, 200 to 300-, 300 to 1000-, 300 to 900-, 300 to 800-, 300 to 700-, 300 to 600-, 300 to 500-, 300 to 400-, 400 to 1000-, 400 to 900-, 400 to 800-, 400 to 700-, 400 to 600-, 400 to 500-, 500 to 1000-, 500 to 900-, 500 to 800-, 500 to 700-, 500 to 600-, 600 to 1000-, 600 to 900-, 600 to 800-, 600 to 700-, 700 to 1000-, 700 to 900-, 700 to 800-, 800 to 1000-, 800 to 900-, or 900 to 1000-fold reduction in the standard of care dose of a recombinant EBV protein vaccine. In some embodiments, such as the foregoing, the anti-EBV antigen antibody titer produced in the subject is equivalent to an anti-EBV antigen antibody titer produced in a control subject administered the standard of care dose of a recombinant or purified EBV protein vaccine, or a live attenuated or inactivated EBV vaccine, or an EBV VLP vaccine. In some embodiments, the effective amount is a dose equivalent to (or equivalent to an at least) 2-, 3-, 4-, 5-, 6-, 7-, 8-, 9-, 10-, 20-, 30-, 40-, 50-, 60-, 70-, 80-, 90-, 100-, 110-, 120-, 130-, 140-, 150-, 160-, 170-, 1280-, 190-, 200-, 210-, 220-, 230-, 240-, 250-, 260-, 270-, 280-, 290-, 300-, 310-, 320-, 330-, 340-, 350-, 360-, 370-, 380-, 390-, 400-, 410-, 420-, 430-, 440-, 450-, 460-, 470-, 480-, 490-, 500-, 510-, 520-, 530-, 540-, 550-, 560-, 570-, 580-, 590-, 600-, 610-, 620-, 630-, 640-, 650-, 660-, 670-, 680-, 690-, 700-, 710-, 720-, 730-, 740-, 750-, 760-, 770-, 780-, 790-, 800-, 810-, 820-, 830-, 840-, 850-, 860-, 870-, 880-, 890-, 900-, 910-, 920-, 930-, 940-, 950-, 960-, 970-, 980-, 990-, or 1000-fold reduction in the standard of care dose of a recombinant EBV protein vaccine. In some embodiments, such as the foregoing, an anti-EBV antigen antibody titer produced in the subject is equivalent to an anti-EBV antigen antibody titer produced in a control subject administered the standard of care dose of a recombinant or purified EBV protein vaccine, or a live attenuated or inactivated EBV vaccine, or an EBV VLP vaccine. 
     In some embodiments, the effective amount of an EBV RNA (e.g., mRNA) vaccine is a total dose of 50-1000 μg. In some embodiments, the effective amount of an EBV RNA (e.g., mRNA) vaccine is a total dose of 50-1000, 50-900, 50-800, 50-700, 50-600, 50-500, 50-400, 50-300, 50-200, 50-100, 50-90, 50-80, 50-70, 50-60, 60-1000, 60-900, 60-800, 60-700, 60-600, 60-500, 60-400, 60-300, 60-200, 60-100, 60-90, 60-80, 60-70, 70-1000, 70-900, 70-800, 70-700, 70-600, 70-500, 70-400, 70-300, 70-200, 70-100, 70-90, 70-80, 80-1000, 80-900, 80-800, 80-700, 80-600, 80-500, 80-400, 80-300, 80-200, 80-100, 80-90, 90-1000, 90-900, 90-800, 90-700, 90-600, 90-500, 90-400, 90-300, 90-200, 90-100, 100-1000, 100-900, 100-800, 100-700, 100-600, 100-500, 100-400, 100-300, 100-200, 200-1000, 200-900, 200-800, 200-700, 200-600, 200-500, 200-400, 200-300, 300-1000, 300-900, 300-800, 300-700, 300-600, 300-500, 300-400, 400-1000, 400-900, 400-800, 400-700, 400-600, 400-500, 500-1000, 500-900, 500-800, 500-700, 500-600, 600-1000, 600-900, 600-900, 600-700, 700-1000, 700-900, 700-800, 800-1000, 800-900, or 900-1000 μg. In some embodiments, the effective amount of an EBV RNA (e.g., mRNA) vaccine is a total dose of 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950 or 1000 μg. In some embodiments, the effective amount is a dose of 25-500 μg administered to the subject a total of two times. In some embodiments, the effective amount of an EBV RNA (e.g., mRNA) vaccine is a dose of 25-500, 25-400, 25-300, 25-200, 25-100, 25-50, 50-500, 50-400, 50-300, 50-200, 50-100, 100-500, 100-400, 100-300, 100-200, 150-500, 150-400, 150-300, 150-200, 200-500, 200-400, 200-300, 250-500, 250-400, 250-300, 300-500, 300-400, 350-500, 350-400, 400-500 or 450-500 μg administered to the subject a total of two times. In some embodiments, the effective amount of an EBV RNA (e.g., mRNA) vaccine is a total dose of 25, 50, 100, 150, 200, 250, 300, 350, 400, 450, or 500 μg administered to the subject a total of two times. 
     Vaccine efficacy may be assessed using standard analyses (see, e.g., Weinberg et al., J Infect Dis. 2010 Jun. 1; 201(11):1607-10). For example, vaccine efficacy may be measured by double-blind, randomized, clinical controlled trials. Vaccine efficacy may be expressed as a proportionate reduction in disease attack rate (AR) between the unvaccinated (ARU) and vaccinated (ARV) study cohorts and can be calculated from the relative risk (RR) of disease among the vaccinated group with use of the following formulas: 
       Efficacy=(ARU−ARV)/ARU×100; and
 
       Efficacy=(1−RR)×100.
 
     Likewise, vaccine effectiveness may be assessed using standard analyses (see, e.g., Weinberg et al., J Infect Dis. 2010 Jun. 1; 201(11):1607-10). Vaccine effectiveness is an assessment of how a vaccine (which may have already proven to have high vaccine efficacy) reduces disease in a population. This measure can assess the net balance of benefits and adverse effects of a vaccination program, not just the vaccine itself, under natural field conditions rather than in a controlled clinical trial. Vaccine effectiveness is proportional to vaccine efficacy (potency) but is also affected by how well target groups in the population are immunized, as well as by other non-vaccine-related factors that influence the ‘real-world’ outcomes of hospitalizations, ambulatory visits, or costs. For example, a retrospective case control analysis may be used, in which the rates of vaccination among a set of infected cases and appropriate controls are compared. Vaccine effectiveness may be expressed as a rate difference, with use of the odds ratio (OR) for developing infection despite vaccination: 
       Effectiveness=(1−OR)×100.
 
     In some embodiments, efficacy of the EBV vaccine is at least 60% relative to unvaccinated control subjects. For example, efficacy of the EBV vaccine may be at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 95%, at least 98%, or 100% relative to unvaccinated control subjects. 
     Sterilizing Immunity. 
     Sterilizing immunity refers to a unique immune status that prevents effective pathogen infection into the host. In some embodiments, the effective amount of an EBV vaccine of the present disclosure is sufficient to provide sterilizing immunity in the subject for at least 1 year. For example, the effective amount of an EBV vaccine of the present disclosure is sufficient to provide sterilizing immunity in the subject for at least 2 years, at least 3 years, at least 4 years, or at least 5 years. In some embodiments, the effective amount of an EBV vaccine of the present disclosure is sufficient to provide sterilizing immunity in the subject at an at least 5-fold lower dose relative to control. For example, the effective amount may be sufficient to provide sterilizing immunity in the subject at an at least 10-fold lower, 15-fold, or 20-fold lower dose relative to a control. 
     Detectable Antigen. 
     In some embodiments, the effective amount of an EBV vaccine of the present disclosure is sufficient to produce detectable levels of EBV antigen as measured in serum of the subject at 1-72 hours post administration. 
     Titer. 
     An antibody titer is a measurement of the amount of antibodies within a subject, for example, antibodies that are specific to a particular antigen (e.g., an anti-EBV antigen). Antibody titer is typically expressed as the inverse of the greatest dilution that provides a positive result. Enzyme-linked immunosorbent assay (ELISA) is a common assay for determining antibody titers, for example. 
     In some embodiments, the effective amount of an EBV vaccine of the present disclosure is sufficient to produce a 1,000-10,000 neutralizing antibody titer produced by neutralizing antibody against the EBV antigen as measured in serum of the subject at 1-72 hours post administration. In some embodiments, the effective amount is sufficient to produce a 1,000-5,000 neutralizing antibody titer produced by neutralizing antibody against the EBV antigen as measured in serum of the subject at 1-72 hours post administration. In some embodiments, the effective amount is sufficient to produce a 5,000-10,000 neutralizing antibody titer produced by neutralizing antibody against the EBV antigen as measured in serum of the subject at 1-72 hours post administration. 
     In some embodiments, the neutralizing antibody titer is at least 100 NT 50 . For example, the neutralizing antibody titer may be at least 200, 300, 400, 500, 600, 700, 800, 900 or 1000 NT 50 . In some embodiments, the neutralizing antibody titer is at least 10,000 NT 50 . 
     In some embodiments, the neutralizing antibody titer is at least 100 neutralizing units per milliliter (NU/mL). For example, the neutralizing antibody titer may be at least 200, 300, 400, 500, 600, 700, 800, 900 or 1000 NU/mL. In some embodiments, the neutralizing antibody titer is at least 10,000 NU/mL. 
     In some embodiments, an anti-EBV antigen antibody titer produced in the subject is increased by at least 1 log relative to a control. For example, an anti-EBV antigen antibody titer produced in the subject may be increased by at least 2, 3, 4, 5, 6, 7, 8, 9 or 10 log relative to a control. 
     In some embodiments, an anti-EBV antigen antibody titer produced in the subject is increased at least 2 times relative to a control. For example, an anti-EBV antigen antibody titer produced in the subject is increased by at least 3, 4, 5, 6, 7, 8, 9 or 10 times relative to a control. 
     In some embodiments, a geometric mean, which is the nth root of the product of n numbers, is generally used to describe proportional growth. Geometric mean, in some embodiments, is used to characterize antibody titer produced in a subject. 
     A control may be, for example, an unvaccinated subject, or a subject administered a live attenuated EBV vaccine, an inactivated EBV vaccine, or a protein subunit EBV vaccine. 
     EXAMPLES 
     Example 1 
     EBV glycoprotein 350 (gp350) variants were produced and their expression tested in HeLa cells. HeLa cells were transiently transfected for 24 hours with mRNA encoding each of EBV glycoprotein 350 (gp350) variants (SEQ ID NOs: 185, 182, 207, and 208). Flow cytometry analyses ( FIG. 1A , 1 μg dose mRNA) and immunoassays ( FIG. 1B , 1 μg dose mRNA;  FIG. 1C , 0.5 μg dose mRNA) using an EBV neutralizing antibody that binds conformational epitopes in gp350 (“72A1”) demonstrate that all EBV gp350 variants tested show equivalent expression at the surface of the transfected HeLa cells. Unless otherwise stated, all mRNA vaccines may be formulated in lipid nanoparticles comprising Compound 1 lipids, e.g., 20-60% ionizable cationic lipid, 5-25% non-cationic lipid, 25-55% sterol, and 0.5-15% PEG-modified lipid. 
     Example 2 
     EBV gp350 mRNA sequences with two different 5′ UTR sequences (SEQ ID NOs: 81 and 204) were produced and their expression tested in HeLa cells. HeLa cells were transiently transfected for 24 hours with a 0.5 μg dose of mRNA encoding the EBV gp350 antigens. Flow cytometry analyses ( FIG. 2A ) and immunoassays ( FIG. 2B ) using the anti-72A1 antibody demonstrate that both EBV gp350 mRNA constructs tested show equivalent expression at the surface of the transfected HeLa cells. 
     Example 3 
     Balb/c mice were vaccinated intramuscularly with EBV vaccines comprising mRNA encoding EBV gp350 variants (SEQ ID NOs: 185, 182, 207, and 208) formulated in a lipid nanoparticle. A 2 μg dose was administered on Day 1 and then again on Day 22. Mice were bled on Day 21 and Day 43. Results demonstrate that all the EBV gp350 vaccines tested induced serum gp350-specific IgG antibody titers at day 21 (3 weeks post prime) and day 43 (3 weeks post boost) following vaccination ( FIG. 3 ). 
     Example 4 
     Additional EBV antigens and antigen complexes were produced and their expression tested in HeLa cells. HeLa cells were transiently transfected for 24 hours with 0.25 μg of (1) mRNA encoding EBV gH (SEQ ID NO: 187), mRNA encoding EBV gL (SEQ ID NO: 188), and mRNA encoding EBV gp42 (SEQ ID NO:189) (EBV gH/gL/gp42 UTR A); (2) mRNA encoding EBV gH (SEQ ID NO: 201), mRNA encoding EBV gL (SEQ ID NO: 202), and mRNA encoding EBV gp42 (SEQ ID NO: 203) (EBV gH/gL/gp42 UTR B); (3) mRNA encoding EBV gH (SEQ ID NO: 187) and mRNA encoding gp42 (SEQ ID NO:189); or (4) mRNA encoding EBV gp42 (SEQ ID NO:189). Flow cytometry analyses ( FIG. 4A ) and immunoassays ( FIG. 4B ) using an anti-gH/gL/gp42 (2D4) antibody demonstrate that all EBV mRNA constructs tested show equivalent expression at the surface of the transfected HeLa cells. 
     Example 5 
     Balb/c mice were vaccinated intramuscularly with EBV vaccines comprising (1) mRNA encoding EBV gH (SEQ ID NO: 187), mRNA encoding EBV gL (SEQ ID NO: 188), and mRNA encoding EBV gp42 (SEQ ID NO: 189); (2) mRNA encoding EBV gp350 (SEQ ID NO: 185); or (3) mRNA encoding EBV gH (SEQ ID NO: 187), mRNA encoding EBV gL (SEQ ID NO: 188), mRNA encoding EBV gp42 (SEQ ID NO: 189), and mRNA encoding EBV gp350 (SEQ ID NO: 185). A 2 μg dose was administered on Day 1 and then again on Day 29. Mice were bled on Day 28 and Day 57. Results demonstrate that all the EBV vaccines tested induced neutralizing antibody titers at day 28 (4 weeks post prime) and day 57 (4 weeks post boost) following vaccination ( FIG. 5 ). 
     Example 6 
     HeLa cells were transiently transfected for 24 hours with 0.5 μg of (1) mRNA encoding EBV gH (SEQ ID NO: 201) and mRNA encoding EBV gL (SEQ ID NO: 202); or (2) mRNA encoding EBV gH only (SEQ ID NO: 201). Flow cytometry analyses ( FIG. 6A ) using an anti-gH/gL (2A8) antibody or an anti-gH/gL/gp42 (2D4) antibody demonstrate that the 2A8 antibody binds specifically to EBV gL expressed on the surface of the HeLa cells (compare to data presented in Example 4). Immunoassays following 24 hour transfection with 0.25 μg of either (1) mRNA encoding EBV mRNA encoding EBV gH (SEQ ID NO: 187) and mRNA encoding EBV gL (SEQ ID NO: 188); or (2) mRNA encoding EBV mRNA encoding EBV gH (SEQ ID NO: 201) and mRNA encoding EBV gL (SEQ ID NO: 202) show comparable expression of both EBV antigen complexes at the surface of HeLa cells. 
     Example 7 
     Three additional immunogenicity studies were performed using various mRNA vaccines of the present disclosure (formulated in lipid nanoparticles) and various antigen-specific antibodies. Balb/c mice were vaccinated intramuscularly with (1) a 5 μg dose or a 1 μg dose of mRNA encoding EBV gH (SEQ ID NO: 187) and mRNA encoding EBV gL (SEQ ID NO: 188); (2) a 7.5 μg dose or a 1.5 μg dose of mRNA encoding EBV gH (SEQ ID NO: 187), mRNA encoding EBV gL (SEQ ID NO: 188), and mRNA encoding EBV gB (SEQ ID NO: 209); (3) a 7.5 μg dose or a 1.5 μg dose of mRNA encoding EBV gH (SEQ ID NO: 187), mRNA encoding EBV gL (SEQ ID NO: 188), and mRNA encoding EBV gp350 (SEQ ID NO: 185); or (4) a 10 μg dose or a 2 μg dose of mRNA encoding EBV gH (SEQ ID NO: 187), mRNA encoding EBV gL (SEQ ID NO: 188), mRNA encoding EBV gB (SEQ ID NO: 209), and mRNA encoding EBV gp350 (SEQ ID NO: 185). Mice were bled on Day 57 Results following detection with anti-gH/gL antibody ( FIG. 7 ), anti-gB antibody ( FIG. 8 ), or anti-gp350 antibody ( FIG. 9 ) demonstrate induction of EBV antigen-specific neutralizing antibodies. 
     Example 8 
     Mice were vaccinated with a 2 μg dose of mRNA encoding one of four EBV latent genes (LMP1 (SEQ ID NO: 179), LMP2 (SEQ ID NO: 181), EBNA1 Δ1-400  (SEQ ID NO: 178) or EBNA3A (SEQ ID NO: 177)) or a combination of all four mRNA vaccines (LMP1 (SEQ ID NO: 179), LMP2 (SEQ ID NO: 181), EBNA1 Δ1-400  (SEQ ID NO: 178) and EBNA3A (SEQ ID NO: 177). Cells were harvested from vaccinated mice and stimulated with LMP1, LMP2, EBNA1 or EBNA3A peptides. All peptide libraries comprise 15mer peptides overlapping by 11 amino acids. CD8 T cell responses are shown in  FIGS. 10A-10D , and CD4 T cell responses are shown in  FIGS. 11A-11D . 
     Example 9 
     mRNA encoding EBV glycoprotein H-glycoprotein L (gH-gL) linked constructs (SEQ ID NO: 218 or 221) were produced and their expression tested in HeLa cells. HeLa cells were transiently transfected for 24 hours with mRNA encoding two EBV gH-gL variants with different linkers (SEQ ID NO: 218 or SEQ ID NO: 221), or with mRNA encoding EBV gH (EBV gH mRNA; SEQ ID NO: 228) and mRNA encoding EBV gL (EBV gL mRNA; SEQ ID NO: 229). Flow cytometry analyses ( FIG. 13A , 0.5 μg dose of EBV gH-gL linked mRNA or 0.25 μg dose of each of EBV gH mRNA and EBV gL mRNA) and immunoassays ( FIG. 13B ; 0.5 μg dose of gH-gL linked construct or 0.25 μg dose of each of gH and gL) using EBV neutralizing antibodies 2A8 and CL40, which binds conformational epitopes in gH, demonstrate that both EBV gH-gL constructs (SEQ ID NO: 218 and 221) tested show expression at the surface of the transfected HeLa cells. 
     Example 10 
     In a further immunogenicity study to test the effect of different untranslated regions (UTRs), mice were vaccinated intramuscularly with lipid nanoparticles (comprising Compound 1 lipids, e.g., 20-60% ionizable cationic lipid, 5-25% non-cationic lipid, 25-55% sterol, and 0.5-15% PEG-modified lipid) comprising (1) 10 μg mRNA encoding gp350 (SEQ ID NO: 185), mRNA encoding gH (SEQ ID NO: 187), mRNA encoding gL (SEQ ID NO: 188), mRNA encoding LMP2 antigen (SEQ ID NO: 181), and mRNA encoding EBNA1 antigen (SEQ ID NO: 178), each of the transcripts comprising UTRA; (2) 10 μg mRNA encoding gp350 (SEQ ID NO: 185), mRNA encoding gH (SEQ ID NO: 187), mRNA encoding gL (SEQ ID NO: 188), mRNA encoding LMP2 antigen (SEQ ID NO: 181), and mRNA encoding EBNA1 antigen (SEQ ID NO: 178), each of the transcripts comprising UTRB; (3) 2 μg mRNA encoding EBNA1 antigen (SEQ ID NO: 178) with UTRB; (4) 2 μg mRNA encoding LMP2 antigen with UTRB; or (5) empty lipid nanoparticles. The mice received one dose on Day 1 and a second dose on Day 29. Blood samples were taken just prior to dosing, and on Day 57. Spleens were collected from a subset of animals on Day 36. Cells were harvested from vaccinated mice and stimulated with peptides from an EBNA1 peptide library ( FIG. 14 ) or an LMP2 library ( FIG. 15 ). The peptide library comprised 15mer peptides overlapping by 11 amino acids. CD4 T cell cytokine responses for the EBV formulations (groups 1 and 2), the EBNA1 formulation (group 3), and the control (empty nanoparticles; group 5) are shown in the top row of  FIG. 14 ; the bottom row of  FIG. 14  shows CD8 T cell responses.  FIG. 15  shows the CD4 T cell responses (top row) and CD8 T cell cytokine responses (bottom row) for EBV formulations (groups 1 and 2), the LMP2 formulation (group 4), and the control (empty nanoparticles; group 5). Similar T cell responses were found between groups regardless of the UTR used. 
     Example 11 
     The constructs were tested in a non-human primate (Rhesus macaque) model. The subjects were vaccinated intramuscularly with lipid nanoparticles comprising (1) 200 μg of mRNA encoding gp350 (SEQ ID NO: 185), mRNA encoding gH (SEQ ID NO: 187), mRNA encoding gL (SEQ ID NO: 188), mRNA encoding LMP2 antigen (SEQ ID NO: 181), and mRNA encoding EBNA1 antigen (SEQ ID NO: 178); (2) 50 μg of mRNA encoding gp350 (SEQ ID NO: 185), mRNA encoding gH (SEQ ID NO: 187), mRNA encoding gL (SEQ ID NO: 188), mRNA encoding LMP2 antigen (SEQ ID NO: 181), and mRNA encoding EBNA1 antigen (SEQ ID NO: 178); or (3) 200 μg of mRNA encoding a control. As shown in  FIG. 16 , the subjects received one dose on day 0 and a second dose on day 28. Blood samples were drawn on days 0, 27, 28, 60, 90, 120, 150, and 180. Results following detection with anti-gp350 titer and anti-gH/gL antibody demonstrate that vaccination with the selected formulations results in increased and durable anti-gp350 and anti-gH/gL antibody titers ( FIG. 16 ). While neutralizing antibody titers were found to be durable at high doses, a significant drop in neutralizing antibody titers at low doses of the EBV vaccine was observed ( FIG. 17 ). 
     Example 12 
     The effect of various downstream processes on EBV vaccine immunogenicity was examined. EBV vaccines comprising gp350 (SEQ ID NO: 185), gH (SEQ ID NO: 187), and gL (SEQ ID NO: 188) were synthesized using different downstream purification processes. The resulting mRNA was used to form doses (10μg, 3μg, and 1 μg doses for each of the four groups; PBS was used as a control group; n=8/group) that were administered to mice on Days 1 and 22. Blood samples were collected on Days 1, 21, 22, 36, 82, and 142. Antibody titers from gp250 ( FIG. 18 ) and for gH/gL ( FIG. 19 ) were measured. 
     EQUIVALENTS 
     All references, patents and patent applications disclosed herein are incorporated by reference with respect to the subject matter for which each is cited, which in some cases may encompass the entirety of the document. 
     The indefinite articles “a” and “an,” as used herein in the specification and in the claims, unless clearly indicated to the contrary, should be understood to mean “at least one.” 
     It should also be understood that, unless clearly indicated to the contrary, in any methods claimed herein that include more than one step or act, the order of the steps or acts of the method is not necessarily limited to the order in which the steps or acts of the method are recited. 
     In the claims, as well as in the specification above, all transitional phrases such as “comprising,” “including,” “carrying,” “having,” “containing,” “involving,” “holding,” “composed of,” and the like are to be understood to be open-ended, i.e., to mean including but not limited to. Only the transitional phrases “consisting of” and “consisting essentially of” shall be closed or semi-closed transitional phrases, respectively, as set forth in the United States Patent Office Manual of Patent Examining Procedures, Section 2111.03. 
     The terms “about” and “substantially” preceding a numerical value mean±10% of the recited numerical value. 
     Where a range of values is provided, each value between the upper and lower ends of the range are specifically contemplated and described herein. 
     The entire contents of International Application Nos. PCT/US2015/02740, PCT/US2016/043348, PCT/US2016/043332, PCT/US2016/058327, PCT/US2016/058324, PCT/US2016/058314, PCT/US2016/058310, PCT/US2016/058321, PCT/US2016/058297, PCT/US2016/058319, and PCT/US2016/058314 are incorporated herein by reference. 
     SEQUENCE LISTING 
     It should be understood that any of the mRNA sequences described herein may include a 5′ UTR and/or a 3′ UTR. The UTR sequences may be selected from the following sequences, or other known UTR sequences may be used. It should also be understood that any of the mRNA constructs described herein may further comprise a polyA tail and/or cap (e.g., 7mG(5′)ppp(5′)NlmpNp). Further, while many of the mRNAs and encoded antigen sequences described herein include a signal peptide and/or a peptide tag (e.g., C-terminal His tag), it should be understood that the indicated signal peptide and/or peptide tag may be substituted for a different signal peptide and/or peptide tag, or the signal peptide and/or peptide tag may be omitted. 
     
       
         
           
               
            
               
                   
               
               
                 5′ UTR: GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUAAGAGCCACC 
               
               
                 (SEQ ID NO: 1) 
               
               
                 5′ UTR: GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUAAGACCCC 
               
               
                 GGCGCCGCCACC (SEQ ID NO: 104) 
               
               
                 3′ UTR: UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCCCCUUGGG 
               
               
                 CCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACCCGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGU 
               
               
                 GGGCGGC (SEQ ID NO: 3) 
               
               
                 3′ UTR: UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCCCCUUGGG 
               
               
                 CCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACCCGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGU 
               
               
                 GGGCGGC (SEQ ID NO: 106) 
               
            
           
           
               
               
               
            
               
                   
                   
                 SEQ 
               
               
                   
                   
                 ID 
               
               
                   
                   
                 NO: 
               
               
                   
               
            
           
           
               
            
               
                 EBNA MO1 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 130 consists of from 5′ end to 3′ end, 
                 130 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 2, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3. 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGCGACGAGGGUCCGGGCACAGGACCCGGUAACGGG 
                   2 
               
               
                 Construct 
                 UUGGGCGAAAAGGGUGACACUAGCGGGCCCGAAGGUUCC 
                   
               
               
                 (excluding 
                 GGCGGAUCUGGCCCGCAACGCAGAGGUGGUGAUAACCAC 
                   
               
               
                 the stop 
                 GGUCGCGGUAGAGGCAGAGGCCGUGGCCGGGGUGGCGGA 
                   
               
               
                 codon) 
                 AGGCCCGGUGCCCCUGGAGGGUCAGGGUCUGGCCCAAGG 
                   
               
               
                   
                 CACCGUGAUGGGGUGCGUCGCCCCCAGAAACGCCCGUCCU 
                   
               
               
                   
                 GUAUCGGCUGCAAGGGCACGCAUGGCGGAACGGGAGGUG 
                   
               
               
                   
                 GCGCAGGAGCCGGAGGUGCAAGAGGUCGCGGGGGGUCAG 
                   
               
               
                   
                 GGGGGAGAGGUAGAGGGGGAUCAGGGGGGCGGAGAGGGC 
                   
               
               
                   
                 GGGGUCGAGAAAGAGCAAGAGGUGGCUCCAGAGAGCGCG 
                   
               
               
                   
                 CUCGGGGUCGCGGGCGAGGCCGGGGUGAAAAACGGCCCC 
                   
               
               
                   
                 GGAGCCCAUCCUCGCAAUCAUCAAGCUCGGGAUCGCCACC 
                   
               
               
                   
                 UCGGCGGCCGCCCCCCGGCAGGCGUCCCUUUUUCCACCCA 
                   
               
               
                   
                 GUGGGCGAAGCCGACUACUUCGAGUACCACCAGGAGGGA 
                   
               
               
                   
                 GGACCGGAUGGUGAGCCGGAUGUUCCUCCCGGCGCCAUC 
                   
               
               
                   
                 GAACAAGGUCCUGCCGAUGAUCCAGGCGAGGGGCCCUCA 
                   
               
               
                   
                 ACAGGCCCCCGAGGGCAGGGGGACGGGGGACGUCGGAAG 
                   
               
               
                   
                 AAAGGAGGCUGGUUUGGCAAGCACCGAGGACAGGGGGGU 
                   
               
               
                   
                 AGCAACCCGAAAUUUGAGAACAUCGCCGAGGGGCUCAGG 
                   
               
               
                   
                 GCUCUCCUAGCCCGUAGCCACGUCGAAAGAACUACCGAU 
                   
               
               
                   
                 GAGGGCACCUGGGUUGCCGGCGUGUUCGUGUACGGGGGU 
                   
               
               
                   
                 AGCAAGACCUCUCUAUACAACCUUCGGAGGGGGACUGCA 
                   
               
               
                   
                 CUUGCUAUCCCCCAAUGCCGCCUCACACCACUGUCUCGCC 
                   
               
               
                   
                 UGCCAUUCGGCAUGGCCCCCGGCCCGGGGCCACAGCCUGG 
                   
               
               
                   
                 GCCCCUACGGGAAUCUAUUGUUUGCUAUUUUAUGGUGUU 
                   
               
               
                   
                 CCUGCAGACCCAUAUCUUCGCAGAAGUCCUGAAAGACGC 
                   
               
               
                   
                 CAUCAAAGACUUAGUUAUGACUAAACCCGCACCCACCUG 
                   
               
               
                   
                 CAACAUUCGCGUGACCGUAUGCAGCUUUGAUGAUGGCGU 
                   
               
               
                   
                 CGAUCUGCCCCCAUGGUUCCCCCCUAUGGUCGAGGGCGCC 
                   
               
               
                   
                 GCCGCCGAAGGGGACGACGGGGAUGACGGGGAUGAAGGC 
                   
               
               
                   
                 GGGGACGGCGAUGAGGGCGAGGAGGGCCAGGAA 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGR 
                   4 
               
               
                 amino acid 
                 GRGRGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCK 
                   
               
               
                 sequence 
                 GTHGGTGGGAGAGGARGRGGSGGRGRGGSGGRRGRGRERA 
                   
               
               
                   
                 RGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPF 
                   
               
               
                   
                 FHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPST 
                   
               
               
                   
                 GPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALL 
                   
               
               
                   
                 ARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQC 
                   
               
               
                   
                 RLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVL 
                   
               
               
                   
                 KDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGA 
                   
               
               
                   
                 AAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_mod_002 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 131 consists of from 5′ end to 3′ end: 
                 131 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 5, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGUGACGAGGGACCUGGGACUGGCCCCGGGAACGGU 
                   5 
               
               
                 Construct 
                 CUGGGUGAGAAAGGUGACACGUCUGGUCCUGAAGGAUCU 
                   
               
               
                 (excluding 
                 GGCGGUUCAGGGCCGCAGCGCAGAGGAGGAGACAACCAC 
                   
               
               
                 the stop 
                 GGUAGAGGACGUGGACGCGGGAGAGGCCGAGGGGGCGGA 
                   
               
               
                 codon) 
                 AGACCUGGUGCACCGGGCGGUUCCGGCUCGGGGCCCCGCC 
                   
               
               
                   
                 ACAGGGAUGGGGUGCGGAGACCACAGAAGCGCCCCUCUU 
                   
               
               
                   
                 GCAUCGGCUGCAAGGGAACCCAUGGGGGUACCGGAGGGG 
                   
               
               
                   
                 GAGCUGGUGCAGGUGGCGCCCGUGGUAGGGGUGGCAGCG 
                   
               
               
                   
                 GGGGGCGUGGAAGAGGUGGUUCAGGCGGACGGAGGGGAC 
                   
               
               
                   
                 GCGGACGUGAACGUGCCCGGGGAGGAUCCCGGGAGAGGG 
                   
               
               
                   
                 CACGGGGCCGCGGCAGAGGCAGAGGGGAGAAGAGACCUA 
                   
               
               
                   
                 GAUCCCCUAGCUCUCAAUCCAGCAGCAGUGGGUCCCCCCC 
                   
               
               
                   
                 GAGGCGACCGCCUCCAGGUAGGCGCCCUUUCUUCCACCCC 
                   
               
               
                   
                 GUUGGCGAGGCAGAUUACUUCGAAUAUCAUCAAGAGGGG 
                   
               
               
                   
                 GGGCCCGAUGGCGAGCCGGACGUACCACCCGGUGCUAUC 
                   
               
               
                   
                 GAGCAAGGUCCGGCUGACGAUCCUGGUGAAGGGCCAAGU 
                   
               
               
                   
                 ACCGGACCUAGGGGGCAGGGCGACGGUGGUAGGCGUAAA 
                   
               
               
                   
                 AAAGGAGGGUGGUUCGGCAAACAUCGCGGCCAGGGAGGG 
                   
               
               
                   
                 AGCAACCCCAAGUUUGAAAAUAUCGCCGAAGGGCUACGA 
                   
               
               
                   
                 GCUUUGCUCGCGAGGUCCCAUGUGGAACGAACUACAGAC 
                   
               
               
                   
                 GAGGGCACCUGGGUGGCUGGUGUCUUCGUUUAUGGCGGG 
                   
               
               
                   
                 AGCAAGACCUCCCUGUAUAAUCUAAGGCGGGGCACAGCC 
                   
               
               
                   
                 CUGGCCAUACCGCAGUGUCGCCUGACACCCCUGUCUCGCC 
                   
               
               
                   
                 UGCCUUUUGGCAUGGCGCCAGGGCCAGGACCACAACCUG 
                   
               
               
                   
                 GGCCCCUGCGCGAAAGUAUUGUGUGUUACUUCAUGGUCU 
                   
               
               
                   
                 UCCUGCAGACACAUAUUUUCGCAGAAGUCCUAAAGGAUG 
                   
               
               
                   
                 CAAUAAAGGAUCUCGUAAUGACGAAGCCCGCCCCAACGU 
                   
               
               
                   
                 GUAAUAUUAGGGUUACGGUCUGUAGUUUCGAUGACGGGG 
                   
               
               
                   
                 UAGAUCUCCCCCCCUGGUUCCCACCUAUGGUCGAGGGUGC 
                   
               
               
                   
                 CGCUGCGGAGGGGGACGAUGGCGACGACGGCGAUGAAGG 
                   
               
               
                   
                 CGGGGAUGGCGACGAGGGGGAGGAGGGACAGGAA 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGR 
                   4 
               
               
                 amino acid 
                 GRGRGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCK 
                   
               
               
                 sequence 
                 GTHGGTGGGAGAGGARGRGGSGGRGRGGSGGRRGRGRERA 
                   
               
               
                   
                 RGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPF 
                   
               
               
                   
                 FHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPST 
                   
               
               
                   
                 GPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALL 
                   
               
               
                   
                 ARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQC 
                   
               
               
                   
                 RLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVL 
                   
               
               
                   
                 KDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGA 
                   
               
               
                   
                 AAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_mod_003 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 132 consists of from 5′ end to 3′ end: 
                 132 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 6, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGUCAGACGAGGGCCCAGGCACGGGCCCUGGAAACGGA 
                   6 
               
               
                 Construct 
                 CUGGGAGAGAAGGGGGAUACCUCAGGGCCAGAGGGUAGU 
                   
               
               
                 (excluding 
                 GGGGGUAGCGGGCCUCAGAGAAGGGGGGGCGACAAUCAC 
                   
               
               
                 the stop 
                 GGUCGGGGAAGAGGGAGAGGUAGGGGUAGAGGAGGAGGC 
                   
               
               
                 codon) 
                 CGUCCCGGGGCUCCUGGCGGUUCCGGGUCCGGCCCUCGCC 
                   
               
               
                   
                 ACAGAGAUGGCGUGAGGAGACCUCAGAAGCGUCCGUCCU 
                   
               
               
                   
                 GUAUUGGGUGCAAGGGGACCCACGGUGGCACCGGUGGCG 
                   
               
               
                   
                 GAGCUGGGGCAGGAGGUGCUAGAGGAAGAGGCGGUUCAG 
                   
               
               
                   
                 GUGGAAGGGGUCGCGGUGGUUCAGGAGGACGGCGCGGCC 
                   
               
               
                   
                 GGGGGAGAGAGCGGGCAAGAGGCGGCAGCAGGGAGCGGG 
                   
               
               
                   
                 CAAGAGGACGAGGGAGGGGCCGAGGCGAGAAGCGACCUA 
                   
               
               
                   
                 GGUCGCCUAGUUCACAGAGCUCCAGUUCUGGGUCGCCCCC 
                   
               
               
                   
                 CCGGCGGCCGCCUCCUGGGCGAAGACCCUUUUUCCACCCC 
                   
               
               
                   
                 GUGGGGGAGGCUGACUACUUCGAGUACCACCAGGAGGGC 
                   
               
               
                   
                 GGCCCAGACGGAGAGCCCGAUGUUCCGCCUGGGGCCAUU 
                   
               
               
                   
                 GAACAAGGCCCUGCAGAUGACCCAGGGGAAGGACCAUCA 
                   
               
               
                   
                 ACCGGCCCUAGGGGACAGGGCGAUGGGGGGAGACGGAAA 
                   
               
               
                   
                 AAGGGAGGGUGGUUCGGGAAGCACCGCGGCCAGGGGGGG 
                   
               
               
                   
                 UCUAAUCCGAAAUUCGAAAACAUUGCCGAGGGCCUCCGU 
                   
               
               
                   
                 GCUUUGUUGGCUAGAUCACACGUGGAAAGAACCACGGAU 
                   
               
               
                   
                 GAAGGCACGUGGGUGGCGGGCGUCUUCGUGUAUGGUGGG 
                   
               
               
                   
                 AGUAAGACCUCCCUGUAUAACCUCAGGCGUGGCACAGCC 
                   
               
               
                   
                 CUUGCCAUCCCACAGUGCAGGCUCACACCCCUUAGCAGGC 
                   
               
               
                   
                 UUCCCUUUGGCAUGGCUCCUGGUCCCGGCCCCCAGCCCGG 
                   
               
               
                   
                 UCCACUGCGAGAAUCCAUUGUAUGUUACUUCAUGGUUUU 
                   
               
               
                   
                 CUUGCAGACACAUAUCUUCGCCGAGGUGCUGAAAGACGC 
                   
               
               
                   
                 CAUUAAAGACCUGGUGAUGACAAAGCCUGCCCCCACGUG 
                   
               
               
                   
                 CAAUAUCCGUGUGACCGUCUGCUCCUUUGAUGACGGAGU 
                   
               
               
                   
                 CGACCUGCCUCCCUGGUUUCCCCCAAUGGUCGAGGGCGCU 
                   
               
               
                   
                 GCGGCGGAAGGGGACGACGGCGAUGAUGGUGACGAGGGC 
                   
               
               
                   
                 GGUGAUGGCGACGAAGGUGAAGAGGGUCAGGAG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGR 
                   4 
               
               
                 amino acid 
                 GRGRGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCK 
                   
               
               
                 sequence 
                 GTHGGTGGGAGAGGARGRGGSGGRGRGGSGGRRGRGRERA 
                   
               
               
                   
                 RGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPF 
                   
               
               
                   
                 FHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPST 
                   
               
               
                   
                 GPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALL 
                   
               
               
                   
                 ARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQC 
                   
               
               
                   
                 RLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVL 
                   
               
               
                   
                 KDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGA 
                   
               
               
                   
                 AAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_mod_004 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 133 consists of from 5′ end to 3′ end: 
                 133 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 7, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGCGACGAGGGUCCAGGCACAGGCCCGGGGAACGGG 
                   7 
               
               
                 Construct 
                 UUGGGAGAAAAAGGGGACACUUCCGGUCCAGAAGGCUCC 
                   
               
               
                 (excluding 
                 GGGGGGAGCGGACCUCAGAGAAGAGGUGGUGAUAAUCAC 
                   
               
               
                 the stop 
                 GGGAGAGGGAGAGGGCGGGGCAGGGGACGUGGGGGCGGU 
                   
               
               
                 codon) 
                 AGACCUGGGGCGCCGGGGGGUUCCGGCUCCGGACCACGG 
                   
               
               
                   
                 CAUAGGGAUGGUGUGCGAAGGCCGCAGAAGCGGCCGUCU 
                   
               
               
                   
                 UGUAUAGGCUGCAAAGGCACUCACGGUGGUACCGGGGGG 
                   
               
               
                   
                 GGAGCAGGAGCAGGAGGCGCUCGAGGUCGGGGCGGGUCC 
                   
               
               
                   
                 GGCGGCAGAGGCCGCGGUGGUUCUGGGGGAAGGAGGGGA 
                   
               
               
                   
                 AGAGGGAGAGAACGCGCCCGCGGAGGCUCUCGCGAACGG 
                   
               
               
                   
                 GCCAGAGGGCGGGGGAGGGGGCGCGGGGAGAAAAGGCCC 
                   
               
               
                   
                 AGGUCGCCAAGUAGUCAGUCCUCCAGUAGCGGCAGCCCA 
                   
               
               
                   
                 CCCCGCAGACCUCCUCCAGGACGACGACCUUUCUUUCAUC 
                   
               
               
                   
                 CCGUCGGCGAGGCCGAUUACUUUGAGUACCAUCAGGAAG 
                   
               
               
                   
                 GGGGGCCUGAUGGAGAACCGGAUGUUCCUCCUGGUGCUA 
                   
               
               
                   
                 UCGAACAGGGCCCCGCCGACGAUCCAGGCGAGGGCCCCAG 
                   
               
               
                   
                 UACGGGCCCUAGAGGCCAGGGAGAUGGAGGUAGAAGGAA 
                   
               
               
                   
                 GAAGGGGGGAUGGUUUGGCAAGCACAGAGGGCAGGGAGG 
                   
               
               
                   
                 UAGUAAUCCCAAAUUUGAGAAUAUUGCCGAGGGUCUGCG 
                   
               
               
                   
                 GGCACUUCUGGCCCGGUCUCAUGUUGAGAGAACGACUGA 
                   
               
               
                   
                 UGAGGGAACAUGGGUGGCUGGCGUCUUUGUGUAUGGCGG 
                   
               
               
                   
                 UAGUAAAACCAGCCUCUACAAUUUACGGCGUGGGACAGC 
                   
               
               
                   
                 GCUAGCUAUUCCGCAAUGCCGGCUGACCCCUUUGUCUAG 
                   
               
               
                   
                 GCUGCCCUUUGGUAUGGCCCCCGGCCCCGGCCCACAGCCU 
                   
               
               
                   
                 GGGCCUUUGCGGGAAAGCAUCGUCUGCUACUUUAUGGUG 
                   
               
               
                   
                 UUCCUCCAGACCCACAUCUUUGCAGAGGUGCUUAAGGAC 
                   
               
               
                   
                 GCCAUAAAGGACCUGGUUAUGACCAAACCCGCCCCUACCU 
                   
               
               
                   
                 GCAAUAUCCGCGUCACGGUGUGCUCCUUCGACGACGGGG 
                   
               
               
                   
                 UUGACUUGCCUCCAUGGUUCCCACCCAUGGUAGAGGGCG 
                   
               
               
                   
                 CUGCUGCCGAGGGGGAUGAUGGAGACGACGGAGACGAGG 
                   
               
               
                   
                 GCGGCGAUGGCGAUGAAGGCGAAGAAGGCCAGGAA 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
               
               
                 Corresponding 
                 MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGR 
                   4 
               
               
                 amino acid 
                 GRGRGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCK 
                   
               
               
                 sequence 
                 GTHGGTGGGAGAGGARGRGGSGGRGRGGSGGRRGRGRERA 
                   
               
               
                   
                 RGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPF 
                   
               
               
                   
                 FHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPST 
                   
               
               
                   
                 GPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALL 
                   
               
               
                   
                 ARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQC 
                   
               
               
                   
                 RLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVL 
                   
               
               
                   
                 KDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGA 
                   
               
               
                   
                 AAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_mod_005 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 134 consists of from 5′ end to 3′ end: 
                 134 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 8, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGUCUGACGAAGGCCCGGGCACAGGCCCAGGCAACGGA 
                   8 
               
               
                 Construct 
                 UUGGGCGAAAAAGGUGAUACAAGCGGGCCAGAAGGGAGC 
                   
               
               
                 (excluding 
                 GGGGGCUCUGGGCCCCAGAGACGGGGGGGUGACAAUCAC 
                   
               
               
                 the stop 
                 GGCCGAGGAAGGGGAAGGGGCAGGGGAAGAGGCGGAGGA 
                   
               
               
                 codon) 
                 CGGCCUGGCGCUCCUGGAGGCUCUGGCUCAGGUCCAAGA 
                   
               
               
                   
                 CAUAGGGAUGGGGUCAGAAGACCACAGAAGAGACCUAGC 
                   
               
               
                   
                 UGUAUUGGAUGCAAGGGUACACACGGUGGCACGGGCGGC 
                   
               
               
                   
                 GGUGCUGGCGCUGGGGGCGCGAGAGGACGUGGAGGUUCA 
                   
               
               
                   
                 GGCGGGAGAGGUCGAGGAGGCUCUGGAGGCCGCAGGGGG 
                   
               
               
                   
                 AGAGGACGAGAGAGGGCCCGGGGGGGGUCCCGGGAAAGA 
                   
               
               
                   
                 GCUAGAGGGAGGGGACGCGGGCGGGGGGAAAAGCGGCCU 
                   
               
               
                   
                 CGCUCUCCUUCUUCUCAAUCUUCCUCCUCUGGUUCUCCGC 
                   
               
               
                   
                 CCCGACGCCCCCCACCGGGCAGGAGGCCAUUUUUUCACCC 
                   
               
               
                   
                 UGUCGGCGAGGCAGACUACUUCGAGUACCACCAAGAAGG 
                   
               
               
                   
                 AGGCCCAGACGGCGAGCCUGAUGUGCCGCCAGGUGCCAU 
                   
               
               
                   
                 CGAGCAGGGGCCAGCUGAUGACCCGGGGGAGGGACCUAG 
                   
               
               
                   
                 CACGGGGCCCCGCGGUCAGGGCGACGGAGGGAGGAGAAA 
                   
               
               
                   
                 AAAAGGUGGGUGGUUUGGCAAGCAUAGAGGGCAGGGUGG 
                   
               
               
                   
                 CUCAAACCCAAAGUUUGAAAACAUCGCAGAAGGUCUUCG 
                   
               
               
                   
                 GGCUCUGCUGGCCCGCUCUCAUGUAGAGCGCACUACUGA 
                   
               
               
                   
                 CGAAGGCACCUGGGUGGCCGGGGUGUUCGUGUACGGAGG 
                   
               
               
                   
                 AUCUAAGACCAGUCUGUACAACCUCCGGAGGGGGACAGC 
                   
               
               
                   
                 UCUCGCUAUCCCCCAGUGUAGGCUCACCCCUUUGAGUCGC 
                   
               
               
                   
                 CUUCCGUUCGGAAUGGCUCCUGGCCCGGGCCCCCAGCCCG 
                   
               
               
                   
                 GGCCACUAAGAGAGAGCAUCGUAUGCUACUUUAUGGUAU 
                   
               
               
                   
                 UUUUGCAAACCCACAUCUUCGCUGAAGUGCUUAAGGACG 
                   
               
               
                   
                 CCAUCAAAGACCUGGUGAUGACAAAACCAGCUCCAACAU 
                   
               
               
                   
                 GCAAUAUUAGGGUAACCGUAUGCUCCUUCGAUGAUGGCG 
                   
               
               
                   
                 UCGACCUUCCACCUUGGUUCCCCCCAAUGGUGGAGGGAG 
                   
               
               
                   
                 CCGCAGCAGAAGGAGACGAUGGUGAUGAUGGAGACGAAG 
                   
               
               
                   
                 GCGGGGAUGGCGACGAAGGAGAGGAGGGACAGGAA 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGR 
                   4 
               
               
                 amino acid 
                 GRGRGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCK 
                   
               
               
                 sequence 
                 GTHGGTGGGAGAGGARGRGGSGGRGRGGSGGRRGRGRERA 
                   
               
               
                   
                 RGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPF 
                   
               
               
                   
                 FHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPST 
                   
               
               
                   
                 GPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALL 
                   
               
               
                   
                 ARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQC 
                   
               
               
                   
                 RLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVL 
                   
               
               
                   
                 KDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGA 
                   
               
               
                   
                 AAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_trunc_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 135 consists of from 5′ end to 3′ end: 
                 135 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 9, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGCAGAAGACCCUUCUUUCACCCCGUGGGAGAAGCC 
                   9 
               
               
                 Construct 
                 GACUAUUUCGAGUACCACCAGGAGGGCGGUCCUGACGGC 
                   
               
               
                 (excluding 
                 GAGCCCGACGUGCCUCCAGGCGCCAUCGAACAGGGCCCCG 
                   
               
               
                 the stop 
                 CUGACGAUCCUGGCGAGGGGCCUUCCACAGGCCCUCGCGG 
                   
               
               
                 codon) 
                 CCAGGGAGAUGGGGGAAGGCGGAAGAAGGGGGGCUGGUU 
                   
               
               
                   
                 UGGCAAGCAUAGGGGCCAGGGCGGCUCCAAUCCCAAGUU 
                   
               
               
                   
                 CGAGAAUAUCGCCGAGGGGCUCCGGGCUCUGCUGGCCCG 
                   
               
               
                   
                 GUCGCACGUCGAACGUACCACUGACGAGGGGACGUGGGU 
                   
               
               
                   
                 GGCCGGCGUCUUUGUGUACGGAGGAUCCAAGACCUCCCU 
                   
               
               
                   
                 GUACAACCUGAGAAGGGGGACCGCCCUUGCCAUCCCUCA 
                   
               
               
                   
                 GUGCAGACUCACCCCCCUGAGCAGGCUCCCCUUUGGGAUG 
                   
               
               
                   
                 GCUCCUGGGCCAGGCCCCCAGCCCGGGCCCCUCAGGGAAA 
                   
               
               
                   
                 GCAUCGUGUGUUACUUUAUGGUGUUUCUGCAGACACACA 
                   
               
               
                   
                 UUUUUGCAGAAGUUCUGAAAGAUGCAAUCAAGGAUCUGG 
                   
               
               
                   
                 UGAUGACCAAGCCUGCACCUACGUGCAAUAUCAGGGUGA 
                   
               
               
                   
                 CAGUAUGCUCCUUCGACGACGGUGUGGACCUCCCCCCCUG 
                   
               
               
                   
                 GUUCCCACCCAUGGUCGAGGGGGCCGCCGCCGAGGGAGA 
                   
               
               
                   
                 CGAUGGCGACGACGGUGAUGAGGGGGGAGACGGAGAUGA 
                   
               
               
                   
                 AGGCGAGGAGGGGCAGGAG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADD 
                  10 
               
               
                 amino acid 
                 PGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIA 
                   
               
               
                 sequence 
                 EGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRG 
                   
               
               
                   
                 TALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQ 
                   
               
               
                   
                 THIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWF 
                   
               
               
                   
                 PPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_trunc_002 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 136 consists of from 5′ end to 3′ end: 
                 136 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 11, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGACGGAGACCAUUCUUCCACCCCGUGGGAGAAGCC 
                  11 
               
               
                 Construct 
                 GACUACUUCGAGUACCACCAGGAGGGCGGUCCAGACGGC 
                   
               
               
                 (excluding 
                 GAACCCGACGUCCCUCCUGGAGCCAUCGAGCAGGGCCCCG 
                   
               
               
                 the stop 
                 CCGACGACCCUGGCGAGGGCCCUAGCACCGGACCUAGAGG 
                   
               
               
                 codon) 
                 ACAAGGCGACGGCGGGAGGAGAAAGAAGGGGGGCUGGUU 
                   
               
               
                   
                 CGGGAAGCACCGCGGGCAAGGCGGCAGCAACCCAAAGUU 
                   
               
               
                   
                 CGAGAACAUCGCCGAGGGGUUGCGGGCCCUGCUCGCCCG 
                   
               
               
                   
                 UUCCCACGUGGAGCGGACAACCGACGAGGGAACCUGGGU 
                   
               
               
                   
                 UGCCGGGGUAUUUGUGUACGGCGGCAGUAAGACCAGCCU 
                   
               
               
                   
                 GUAUAACCUCAGAAGAGGAACAGCCCUGGCCAUCCCACA 
                   
               
               
                   
                 GUGCAGGCUGACACCCCUCUCCCGGCUGCCAUUUGGAAU 
                   
               
               
                   
                 GGCACCCGGGCCUGGACCCCAGCCUGGCCCACUGCGCGAG 
                   
               
               
                   
                 UCUAUCGUGUGUUACUUUAUGGUCUUCCUGCAAACCCAC 
                   
               
               
                   
                 AUCUUCGCCGAGGUUUUAAAGGAUGCCAUCAAAGACCUC 
                   
               
               
                   
                 GUGAUGACAAAGCCCGCUCCCACCUGCAAUAUCAGAGUG 
                   
               
               
                   
                 ACCGUAUGCAGCUUUGACGACGGCGUGGACCUGCCUCCC 
                   
               
               
                   
                 UGGUUCCCUCCUAUGGUGGAGGGCGCCGCCGCAGAAGGG 
                   
               
               
                   
                 GACGACGGUGACGAUGGCGAUGAGGGAGGGGAUGGAGAC 
                   
               
               
                   
                 GAAGGCGAGGAGGGCCAGGAG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADD 
                  10 
               
               
                 amino acid 
                 PGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIA 
                   
               
               
                 sequence 
                 EGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRG 
                   
               
               
                   
                 TALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQ 
                   
               
               
                   
                 THIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWF 
                   
               
               
                   
                 PPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_trunc_003 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 137 consists of from 5′ end to 3′ end: 
                 137 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 12, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGCCGCAGACCCUUCUUUCACCCCGUGGGAGAGGCC 
                  12 
               
               
                 Construct 
                 GACUAUUUUGAGUACCACCAGGAGGGCGGGCCCGACGGC 
                   
               
               
                 (excluding 
                 GAACCUGACGUGCCACCUGGCGCCAUUGAACAAGGCCCA 
                   
               
               
                 the stop 
                 GCCGACGACCCUGGGGAGGGCCCUAGCACAGGGCCCAGG 
                   
               
               
                 codon) 
                 GGCCAGGGAGACGGGGGUAGGCGUAAGAAGGGAGGCUGG 
                   
               
               
                   
                 UUCGGUAAGCACCGCGGCCAGGGUGGCAGCAACCCUAAA 
                   
               
               
                   
                 UUCGAGAAUAUUGCCGAAGGUCUGCGAGCCCUCUUGGCC 
                   
               
               
                   
                 AGAAGCCACGUGGAACGCACUACUGAUGAGGGCACCUGG 
                   
               
               
                   
                 GUCGCCGGAGUGUUCGUGUACGGCGGAAGCAAAACCAGU 
                   
               
               
                   
                 CUGUACAACCUGCGCCGGGGAACCGCUCUUGCCAUCCCCC 
                   
               
               
                   
                 AGUGUCGCCUGACCCCAUUGUCCAGACUGCCAUUUGGCA 
                   
               
               
                   
                 UGGCCCCCGGCCCCGGCCCCCAGCCCGGCCCCCUGAGGGA 
                   
               
               
                   
                 GAGCAUCGUGUGCUACUUCAUGGUGUUCCUGCAGACCCA 
                   
               
               
                   
                 CAUAUUCGCUGAGGUGCUGAAGGACGCCAUUAAGGACCU 
                   
               
               
                   
                 GGUGAUGACCAAGCCCGCCCCUACCUGCAACAUCCGCGUC 
                   
               
               
                   
                 ACCGUCUGUAGUUUCGAUGACGGCGUGGAUCUCCCACCA 
                   
               
               
                   
                 UGGUUUCCUCCAAUGGUGGAGGGCGCUGCUGCCGAGGGG 
                   
               
               
                   
                 GACGACGGAGACGACGGAGACGAGGGCGGGGACGGGGAC 
                   
               
               
                   
                 GAAGGCGAAGAGGGGCAAGAG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADD 
                  10 
               
               
                 amino acid 
                 PGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIA 
                   
               
               
                 sequence 
                 EGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRG 
                   
               
               
                   
                 TALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQ 
                   
               
               
                   
                 THIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWF 
                   
               
               
                   
                 PPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_trunc_004 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 138 consists of from 5′ end to 3′ end: 
                 138 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 13, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGACGGAGACCUUUCUUUCACCCCGUAGGUGAAGCC 
                  13 
               
               
                 Construct 
                 GACUACUUUGAAUACCACCAGGAGGGCGGCCCAGACGGC 
                   
               
               
                 (excluding 
                 GAGCCCGACGUUCCCCCCGGGGCUAUCGAGCAGGGGCCUG 
                   
               
               
                 the stop 
                 CGGACGACCCAGGAGAGGGACCUAGCACUGGACCCCGAG 
                   
               
               
                 codon) 
                 GUCAGGGCGAUGGGGGGCGAAGAAAGAAGGGCGGCUGGU 
                   
               
               
                   
                 UCGGCAAACAUAGGGGCCAGGGCGGGUCUAACCCAAAGU 
                   
               
               
                   
                 UCGAGAACAUAGCCGAGGGCCUGAGAGCCCUGCUGGCAA 
                   
               
               
                   
                 GAAGCCACGUGGAGCGAACCACAGAUGAAGGCACCUGGG 
                   
               
               
                   
                 UGGCCGGCGUGUUCGUGUACGGGGGCAGCAAGACCUCCC 
                   
               
               
                   
                 UGUAUAACCUCAGAAGAGGGACCGCCCUGGCCAUCCCCCA 
                   
               
               
                   
                 GUGCCGACUGACCCCCCUGAGCAGGCUGCCCUUCGGAAUG 
                   
               
               
                   
                 GCCCCUGGUCCCGGUCCCCAACCGGGCCCGCUUCGGGAGU 
                   
               
               
                   
                 CCAUAGUGUGCUACUUCAUGGUGUUCCUGCAGACACACA 
                   
               
               
                   
                 UCUUCGCCGAGGUGCUGAAGGAUGCAAUCAAGGACCUGG 
                   
               
               
                   
                 UGAUGACCAAGCCAGCUCCCACCUGCAACAUCCGGGUGAC 
                   
               
               
                   
                 CGUGUGCAGCUUUGAUGACGGCGUGGACCUGCCACCCUG 
                   
               
               
                   
                 GUUCCCGCCUAUGGUGGAGGGCGCUGCAGCCGAGGGCGA 
                   
               
               
                   
                 CGACGGGGACGACGGCGACGAGGGCGGAGAUGGCGACGA 
                   
               
               
                   
                 GGGCGAGGAGGGCCAGGAG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADD 
                  10 
               
               
                 amino acid 
                 PGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIA 
                   
               
               
                 sequence 
                 EGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRG 
                   
               
               
                   
                 TALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQ 
                   
               
               
                   
                 THIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWF 
                   
               
               
                   
                 PPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_trunc_005 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 139 consists of from 5′ end to 3′ end: 
                 139 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 14, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGCAGACGGCCCUUCUUCCACCCCGUGGGCGAAGCA 
                  14 
               
               
                 Construct 
                 GACUAUUUUGAGUAUCACCAGGAGGGGGGCCCCGACGGC 
                   
               
               
                 (excluding 
                 GAGCCAGACGUCCCACCUGGUGCAAUCGAGCAAGGUCCC 
                   
               
               
                 the stop 
                 GCCGACGACCCUGGGGAGGGCCCCAGCACCGGCCCCAGAG 
                   
               
               
                 codon) 
                 GCCAGGGGGACGGGGGCAGGAGAAAAAAGGGUGGCUGGU 
                   
               
               
                   
                 UCGGCAAGCACAGAGGGCAGGGGGGGAGCAACCCAAAGU 
                   
               
               
                   
                 UCGAGAACAUCGCCGAGGGCUUGAGAGCACUGCUGGCCA 
                   
               
               
                   
                 GAAGCCACGUGGAGAGAACCACCGAUGAGGGAACCUGGG 
                   
               
               
                   
                 UUGCUGGCGUGUUCGUCUACGGCGGCAGCAAGACCAGCC 
                   
               
               
                   
                 UGUACAACCUUAGAAGAGGCACCGCCCUGGCCAUCCCCCA 
                   
               
               
                   
                 GUGCCGCCUGACACCGCUGAGCAGACUCCCCUUCGGCAUG 
                   
               
               
                   
                 GCUCCUGGCCCCGGCCCCCAGCCCGGGCCCCUGAGAGAGA 
                   
               
               
                   
                 GCAUCGUGUGCUACUUCAUGGUGUUUCUUCAGACGCACA 
                   
               
               
                   
                 UCUUCGCUGAGGUCCUGAAAGACGCCAUUAAGGACCUGG 
                   
               
               
                   
                 UGAUGACAAAACCAGCUCCGACCUGCAACAUCAGGGUCA 
                   
               
               
                   
                 CUGUCUGUAGCUUCGACGAUGGCGUGGAUCUCCCUCCCU 
                   
               
               
                   
                 GGUUCCCCCCAAUGGUGGAGGGCGCUGCCGCUGAGGGGG 
                   
               
               
                   
                 ACGACGGCGACGACGGGGAUGAAGGCGGCGACGGCGACG 
                   
               
               
                   
                 AGGGGGAAGAGGGCCAGGAG 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADD 
                  10 
               
               
                 amino acid 
                 PGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIA 
                   
               
               
                 sequence 
                 EGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRG 
                   
               
               
                   
                 TALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQ 
                   
               
               
                   
                 THIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWF 
                   
               
               
                   
                 PPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP1_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 140 consists of from 5′ end to 3′ end: 
                 140 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 15, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGCACGACCUGGAGAGGGGGCCACCAGGCCCCAGG 
                  15 
               
               
                 Construct 
                 CGACCCCCAAGAGGACCACCACUGAGCUCCAGCCUGGGAC 
                   
               
               
                 (excluding 
                 UGGCCCUCCUGCUGUUGCUCCUCGCUUUACUGUUUUGGC 
                   
               
               
                 the stop 
                 UGUAUAUAGUCAUGUCUGACUGGACAGGCGGGGCAUUAU 
                   
               
               
                 codon) 
                 UGGUGCUCUACAGCUUUGCACUGAUGCUCAUUAUCAUUA 
                   
               
               
                   
                 UUCUGAUUAUCUUUAUUUUUAGACGGGACUUGCUCUGUC 
                   
               
               
                   
                 CAUUAGGCGCGCUGUGUAUUUUGCUGCUGAUGAUCACAC 
                   
               
               
                   
                 UGCUGCUGAUUGCGCUGUGGAACCUCCACGGUCAGGCCC 
                   
               
               
                   
                 UUUUUCUCGGCAUCGUGCUGUUUAUCUUUGGGUGCCUCU 
                   
               
               
                   
                 UGGUGCUUGGGAUUUGGAUCUACCUUCUCGAAAUGUUAU 
                   
               
               
                   
                 GGCGGCUCGGCGCUACAAUCUGGCAGCUCCUGGCCUUCU 
                   
               
               
                   
                 UCUUAGCAUUUUUUCUGGAUCUGAUCCUGCUGAUAAUAG 
                   
               
               
                   
                 CCCUGUACCUACAACAGAACUGGUGGACUCUGCUGGUUG 
                   
               
               
                   
                 ACCUGUUAUGGCUGCUCCUGUUCCUCGCCAUUCUCAUUU 
                   
               
               
                   
                 GGAUGUACUACCACGGGCAGCGUCAUUCAGACGAACACC 
                   
               
               
                   
                 ACCACGAUGAUAGCCUGCCGCACCCUCAACAAGCUACGGA 
                   
               
               
                   
                 UGACUCCGGACACGAGUCCGAUAGUAACUCCAACGAGGG 
                   
               
               
                   
                 ACGCCAUCACUUGCUGGUCUCUGGGGCUGGCGACGGGCC 
                   
               
               
                   
                 UCCACUGUGUAGCCAAAAUCUGGGAGCCCCCGGCGGGGG 
                   
               
               
                   
                 CCCUGAUAAUGGGCCUCAGGAUCCUGAUAACACGGAUGA 
                   
               
               
                   
                 UAACGGCCCACAGGAUCCAGAUAAUACCGACGAUAAUGG 
                   
               
               
                   
                 ACCUCAUGAUCCUCUUCCACAGGAUCCCGACAAUACAGA 
                   
               
               
                   
                 UGACAAUGGGCCGCAGGAUCCAGACAAUACUGACGAUAA 
                   
               
               
                   
                 UGGCCCACAUGAUCCCCUGCCACACAGCCCCUCAGACUCA 
                   
               
               
                   
                 GCGGGAAAUGAUGGCGGGCCCCCACAGUUAACCGAGGAA 
                   
               
               
                   
                 GUGGAAAACAAAGGGGGAGAUCAGGGUCCUCCCCUCAUG 
                   
               
               
                   
                 ACAGAUGGCGGGGGCGGCCACAGUCAUGAUAGUGGGCAC 
                   
               
               
                   
                 GGGGGUGGCGAUCCACAUCUCCCCACACUGCUGCUAGGC 
                   
               
               
                   
                 AGCUCCGGUAGCGGGGGAGAUGAUGACGAUCCCCACGGC 
                   
               
               
                   
                 CCUGUGCAGUUGAGCUAUUACGAC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEHDLERGPPGPRRPPRGPPLSSSLGLALLLLLLALLFWLYIV 
                  16 
               
               
                 amino acid 
                 MSDWTGGALLVLYSFALMLIIIILIIFIFRRDLLCPLGALCILLLM 
                   
               
               
                 sequence 
                 ITLLLIALWNLHGQALFLGIVLFIFGCLLVLGIWIYLLEMLWRL 
                   
               
               
                   
                 GATIWQLLAFFLAFFLDLILLIIALYLQQNWWTLLVDLLWLLL 
                   
               
               
                   
                 FLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSN 
                   
               
               
                   
                 SNEGRHHLLVSGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTD 
                   
               
               
                   
                 DNGPQDPDNTDDNGPHDPLPQDPDNTDDNGPQDPDNTDDNG 
                   
               
               
                   
                 PHDPLPHSPSDSAGNDGGPPQLTEEVENKGGDQGPPLMTDGG 
                   
               
               
                   
                 GGHSHDSGHGGGDPHLPTLLLGSSGSGGDDDDPHGPVQLSYY 
                   
               
               
                   
                 D 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP1_002 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 141 consists of from 5′ end to 3′ end: 
                 141 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 17, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGCACGACCUGGAGCGCGGGCCUCCAGGACCCCGAC 
                  17 
               
               
                 Construct 
                 GGCCGCCCAGGGGGCCACCGCUGUCAUCCAGUCUGGGCCU 
                   
               
               
                 (excluding 
                 UGCUCUGUUGCUGUUACUCCUUGCCCUGCUAUUCUGGUU 
                   
               
               
                 the stop 
                 GUACAUCGUCAUGAGCGAUUGGACAGGGGGUGCUCUGCU 
                   
               
               
                 codon) 
                 GGUGUUGUACAGCUUUGCGCUGAUGUUGAUUAUUAUCAU 
                   
               
               
                   
                 CCUGAUUAUCUUUAUAUUUCGAAGGGACCUCCUUUGUCC 
                   
               
               
                   
                 CCUGGGAGCGCUGUGCAUACUGCUCUUAAUGAUCACGUU 
                   
               
               
                   
                 GCUCCUCAUUGCUUUAUGGAAUCUGCACGGACAGGCUCU 
                   
               
               
                   
                 AUUCCUCGGCAUCGUUUUAUUCAUUUUCGGGUGCCUCCU 
                   
               
               
                   
                 UGUGCUGGGAAUCUGGAUCUAUCUGCUAGAGAUGCUGUG 
                   
               
               
                   
                 GCGGCUCGGCGCCACCAUUUGGCAGCUUCUGGCAUUUUU 
                   
               
               
                   
                 CCUAGCGUUUUUUCUGGAUCUCAUCCUACUUAUUAUCGC 
                   
               
               
                   
                 CUUGUAUUUGCAGCAGAACUGGUGGACUCUCCUGGUGGA 
                   
               
               
                   
                 UCUCCUGUGGCUCCUCCUGUUCCUCGCCAUAUUGAUAUG 
                   
               
               
                   
                 GAUGUACUACCACGGACAGCGCCACAGCGACGAGCACCAC 
                   
               
               
                   
                 CACGAUGAUAGUCUGCCUCACCCUCAACAGGCUACAGAU 
                   
               
               
                   
                 GACUCAGGCCACGAAAGCGAUUCCAACUCAAAUGAAGGC 
                   
               
               
                   
                 CGGCACCACUUGUUGGUGUCCGGAGCCGGCGACGGGCCA 
                   
               
               
                   
                 CCACUCUGCAGCCAGAAUCUGGGCGCUCCCGGCGGAGGUC 
                   
               
               
                   
                 CAGACAAUGGGCCACAGGACCCUGAUAACACGGAUGAUA 
                   
               
               
                   
                 ACGGACCGCAGGAUCCUGACAAUACAGAUGACAACGGCC 
                   
               
               
                   
                 CGCAUGAUCCACUGCCUCAGGAUCCUGAUAAUACUGACG 
                   
               
               
                   
                 AUAACGGACCCCAGGAUCCUGAUAACACCGACGACAACG 
                   
               
               
                   
                 GGCCUCACGAUCCCCUACCCCAUUCCCCAUCUGACUCUGC 
                   
               
               
                   
                 UGGCAACGAUGGCGGCCCACCGCAGCUGACCGAAGAGGU 
                   
               
               
                   
                 AGAAAACAAGGGCGGGGAUCAGGGCCCUCCACUGAUGAC 
                   
               
               
                   
                 AGACGGCGGCGGGGGACACUCCCAUGAUUCGGGCCAUGG 
                   
               
               
                   
                 GGGAGGGGAUCCUCACCUUCCCACACUCUUGCUGGGAAG 
                   
               
               
                   
                 UAGCGGGUCCGGCGGGGACGAUGAUGAUCCCCAUGGUCC 
                   
               
               
                   
                 AGUCCAGUUGUCAUAUUACGAU 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEHDLERGPPGPRRPPRGPPLSSSLGLALLLLLLALLFWLYIV 
                  16 
               
               
                 amino acid 
                 MSDWTGGALLVLYSFALMLIIIILIIFIFRRDLLCPLGALCILLLM 
                   
               
               
                 sequence 
                 ITLLLIALWNLHGQALFLGIVLFIFGCLLVLGIWIYLLEMLWRL 
                   
               
               
                   
                 GATIWQLLAFFLAFFLDLILLIIALYLQQNWWTLLVDLLWLLL 
                   
               
               
                   
                 FLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSN 
                   
               
               
                   
                 SNEGRHHLLVSGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTD 
                   
               
               
                   
                 DNGPQDPDNTDDNGPHDPLPQDPDNTDDNGPQDPDNTDDNG 
                   
               
               
                   
                 PHDPLPHSPSDSAGNDGGPPQLTEEVENKGGDQGPPLMTDGG 
                   
               
               
                   
                 GGHSHDSGHGGGDPHLPTLLLGSSGSGGDDDDPHGPVQLSYY 
                   
               
               
                   
                 D 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP1_003 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 142 consists of from 5′ end to 3′ end: 
                 142 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 18, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGCACGACCUUGAGCGGGGUCCACCAGGCCCACGG 
                  18 
               
               
                 Construct 
                 CGCCCUCCGCGCGGGCCUCCUUUGUCAUCCUCCCUGGGAC 
                   
               
               
                 (excluding 
                 UGGCACUGCUGCUGUUGCUUCUGGCCCUGCUGUUCUGGC 
                   
               
               
                 the stop 
                 UGUACAUCGUUAUGUCUGAUUGGACAGGAGGAGCUCUGC 
                   
               
               
                 codon) 
                 UGGUUCUUUACUCUUUCGCGCUGAUGCUGAUCAUCAUCA 
                   
               
               
                   
                 UCUUGAUCAUUUUCAUUUUCAGACGAGACCUCCUUUGUC 
                   
               
               
                   
                 CUCUCGGAGCCCUGUGUAUCCUCCUGCUGAUGAUAACCC 
                   
               
               
                   
                 UUCUGCUGAUUGCUUUGUGGAAUCUGCAUGGGCAAGCUC 
                   
               
               
                   
                 UGUUUCUUGGAAUUGUCUUAUUUAUUUUUGGGUGUCUGC 
                   
               
               
                   
                 UGGUGUUGGGAAUCUGGAUCUAUCUUCUUGAAAUGCUGU 
                   
               
               
                   
                 GGCGGCUGGGCGCCACAAUCUGGCAGCUACUCGCCUUUU 
                   
               
               
                   
                 UCCUAGCUUUCUUUUUGGAUCUGAUUCUGCUGAUUAUUG 
                   
               
               
                   
                 CUCUCUACCUGCAGCAGAAUUGGUGGACCCUCUUAGUCG 
                   
               
               
                   
                 AUCUGCUGUGGCUGUUGCUCUUUCUCGCCAUCCUGAUUU 
                   
               
               
                   
                 GGAUGUACUAUCAUGGGCAGCGCCAUAGUGAUGAACAUC 
                   
               
               
                   
                 AUCACGAUGAUUCCCUGCCACAUCCUCAGCAGGCCACAGA 
                   
               
               
                   
                 UGAUUCAGGCCACGAAUCAGACAGCAAUAGCAAUGAGGG 
                   
               
               
                   
                 CCGCCACCACCUGCUGGUUUCAGGGGCCGGUGACGGCCCU 
                   
               
               
                   
                 CCAUUGUGCAGUCAGAACCUGGGAGCCCCGGGGGGUGGA 
                   
               
               
                   
                 CCUGAUAACGGGCCGCAGGAUCCUGACAAUACUGAUGAC 
                   
               
               
                   
                 AACGGACCCCAGGAUCCAGAUAACACUGACGACAAUGGC 
                   
               
               
                   
                 CCCCAUGACCCCCUACCCCAGGAUCCCGACAAUACAGACG 
                   
               
               
                   
                 ACAACGGACCGCAGGACCCCGACAACACUGAUGAUAAUG 
                   
               
               
                   
                 GGCCACACGAUCCUCUGCCUCACUCUCCAUCAGACUCUGC 
                   
               
               
                   
                 CGGAAAUGACGGUGGACCGCCACAGUUAACUGAAGAAGU 
                   
               
               
                   
                 CGAAAACAAGGGCGGCGAUCAGGGGCCGCCGCUCAUGAC 
                   
               
               
                   
                 AGAUGGCGGGGGAGGUCACAGCCACGAUAGCGGACACGG 
                   
               
               
                   
                 AGGAGGUGACCCUCAUCUGCCUACACUUCUGUUGGGUAG 
                   
               
               
                   
                 CUCUGGGAGUGGGGGGGAUGACGACGACCCUCACGGACC 
                   
               
               
                   
                 UGUUCAGCUCUCAUACUACGAC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEHDLERGPPGPRRPPRGPPLSSSLGLALLLLLLALLFWLYIV 
                  16 
               
               
                 amino acid 
                 MSDWTGGALLVLYSFALMLIIIILIIFIFRRDLLCPLGALCILLLM 
                   
               
               
                 sequence 
                 ITLLLIALWNLHGQALFLGIVLFIFGCLLVLGIWIYLLEMLWRL 
                   
               
               
                   
                 GATIWQLLAFFLAFFLDLILLIIALYLQQNWWTLLVDLLWLLL 
                   
               
               
                   
                 FLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSN 
                   
               
               
                   
                 SNEGRHHLLVSGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTD 
                   
               
               
                   
                 DNGPQDPDNTDDNGPHDPLPQDPDNTDDNGPQDPDNTDDNG 
                   
               
               
                   
                 PHDPLPHSPSDSAGNDGGPPQLTEEVENKGGDQGPPLMTDGG 
                   
               
               
                   
                 GGHSHDSGHGGGDPHLPTLLLGSSGSGGDDDDPHGPVQLSYY 
                   
               
               
                   
                 D 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP1_004 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 143 consists of from 5′ end to 3′ end: 
                 143 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 19, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAACACGAUCUUGAACGGGGGCCGCCGGGACCGAGG 
                  19 
               
               
                 Construct 
                 AGGCCGCCACGGGGGCCCCCCCUGAGCAGUAGCUUGGGGC 
                   
               
               
                 (excluding 
                 UUGCGCUGCUUCUCCUGCUGUUGGCCCUCCUGUUUUGGC 
                   
               
               
                 the stop 
                 UGUAUAUUGUGAUGUCCGACUGGACCGGCGGCGCCCUGC 
                   
               
               
                 codon) 
                 UCGUCCUGUAUAGCUUUGCUUUGAUGCUGAUAAUUAUUA 
                   
               
               
                   
                 UAUUGAUCAUCUUUAUUUUCCGGCGUGAUUUGCUCUGUC 
                   
               
               
                   
                 CUCUUGGUGCCCUUUGUAUCCUGCUGUUAAUGAUCACUC 
                   
               
               
                   
                 UGCUCCUCAUUGCCCUAUGGAACCUCCACGGACAAGCCCU 
                   
               
               
                   
                 GUUUCUUGGCAUCGUUCUCUUUAUAUUCGGAUGCCUCUU 
                   
               
               
                   
                 AGUGCUUGGGAUCUGGAUAUACCUGUUGGAAAUGCUGUG 
                   
               
               
                   
                 GAGGCUUGGAGCCACGAUAUGGCAAUUAUUGGCAUUCUU 
                   
               
               
                   
                 UCUGGCCUUCUUCCUGGAUCUGAUCCUGUUGAUAAUUGC 
                   
               
               
                   
                 UCUGUACCUGCAGCAGAACUGGUGGACACUACUUGUUGA 
                   
               
               
                   
                 CCUGCUCUGGCUACUCCUGUUCCUUGCGAUCCUGAUUUG 
                   
               
               
                   
                 GAUGUACUAUCACGGACAGAGACACUCCGACGAGCAUCA 
                   
               
               
                   
                 UCACGACGAUUCCUUACCACACCCUCAGCAGGCGACAGAU 
                   
               
               
                   
                 GAUUCUGGCCAUGAGAGCGAUUCCAACAGCAACGAGGGC 
                   
               
               
                   
                 CGGCACCAUUUGCUGGUGUCGGGGGCUGGAGACGGGCCU 
                   
               
               
                   
                 CCGUUAUGUAGCCAAAACUUGGGCGCGCCCGGCGGCGGG 
                   
               
               
                   
                 CCCGAUAACGGCCCGCAGGACCCGGACAAUACCGACGAUA 
                   
               
               
                   
                 AUGGGCCUCAAGAUCCCGACAAUACUGAUGACAAUGGAC 
                   
               
               
                   
                 CACACGAUCCUCUGCCACAGGAUCCCGACAACACAGAUGA 
                   
               
               
                   
                 CAAUGGGCCGCAAGACCCCGACAAUACAGACGAUAAUGG 
                   
               
               
                   
                 CCCCCACGACCCACUACCACAUUCUCCCUCUGACAGUGCG 
                   
               
               
                   
                 GGGAAUGAUGGUGGUCCCCCACAACUGACCGAGGAGGUG 
                   
               
               
                   
                 GAGAAUAAGGGGGGGGACCAAGGUCCACCCCUCAUGACU 
                   
               
               
                   
                 GAUGGAGGAGGAGGACACUCGCACGAUUCAGGUCACGGC 
                   
               
               
                   
                 GGGGGGGACCCUCACUUGCCCACUCUGCUGCUGGGCAGC 
                   
               
               
                   
                 AGCGGUUCUGGGGGCGACGAUGAUGAUCCCCACGGCCCG 
                   
               
               
                   
                 GUGCAGCUAAGUUAUUACGAC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEHDLERGPPGPRRPPRGPPLSSSLGLALLLLLLALLFWLYIV 
                  16 
               
               
                 amino acid 
                 MSDWTGGALLVLYSFALMLIIIILIIFIFRRDLLCPLGALCILLLM 
                   
               
               
                 sequence 
                 ITLLLIALWNLHGQALFLGIVLFIFGCLLVLGIWIYLLEMLWRL 
                   
               
               
                   
                 GATIWQLLAFFLAFFLDLILLIIALYLQQNWWTLLVDLLWLLL 
                   
               
               
                   
                 FLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSN 
                   
               
               
                   
                 SNEGRHHLLVSGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTD 
                   
               
               
                   
                 DNGPQDPDNTDDNGPHDPLPQDPDNTDDNGPQDPDNTDDNG 
                   
               
               
                   
                 PHDPLPHSPSDSAGNDGGPPQLTEEVENKGGDQGPPLMTDGG 
                   
               
               
                   
                 GGHSHDSGHGGGDPHLPTLLLGSSGSGGDDDDPHGPVQLSYY 
                   
               
               
                   
                 D 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP1_005 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 144 consists of from 5′ end to 3′ end: 
                 144 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 20, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGCACGAUUUAGAGAGGGGCCCACCUGGCCCCAGG 
                  20 
               
               
                 Construct 
                 CGCCCACCUAGAGGCCCACCCUUGUCGUCGUCAUUAGGGC 
                   
               
               
                 (excluding 
                 UGGCAUUGCUCCUGCUCCUCCUGGCCUUGCUGUUCUGGC 
                   
               
               
                 the stop 
                 UUUACAUCGUGAUGAGCGACUGGACUGGCGGGGCCCUGC 
                   
               
               
                 codon) 
                 UGGUCCUCUAUAGCUUUGCACUGAUGCUGAUUAUAAUCA 
                   
               
               
                   
                 UAUUGAUCAUCUUCAUCUUCCGACGGGAUUUACUUUGCC 
                   
               
               
                   
                 CCCUGGGGGCAUUAUGUAUUCUAUUGCUGAUGAUAACAC 
                   
               
               
                   
                 UUCUGCUUAUUGCUUUAUGGAAUCUACACGGUCAGGCUC 
                   
               
               
                   
                 UUUUUCUGGGAAUCGUGCUCUUUAUCUUUGGAUGCCUUC 
                   
               
               
                   
                 UGGUACUAGGAAUUUGGAUAUACCUCUUAGAAAUGCUGU 
                   
               
               
                   
                 GGCGGCUCGGCGCAACCAUCUGGCAACUCCUUGCGUUUU 
                   
               
               
                   
                 UUCUCGCGUUUUUCCUCGACCUGAUUCUGCUAAUAAUCG 
                   
               
               
                   
                 CCUUAUACCUUCAGCAGAAUUGGUGGACACUCCUGGUGG 
                   
               
               
                   
                 ACCUGCUGUGGCUCCUGCUAUUCCUUGCAAUCCUCAUUU 
                   
               
               
                   
                 GGAUGUAUUACCAUGGCCAGAGGCACUCUGAUGAGCACC 
                   
               
               
                   
                 ACCACGAUGAUAGUCUACCUCAUCCACAGCAGGCUACGG 
                   
               
               
                   
                 ACGAUUCAGGGCAUGAGUCAGAUUCUAAUUCCAACGAAG 
                   
               
               
                   
                 GAAGACACCAUCUGUUGGUGUCAGGGGCCGGAGACGGCC 
                   
               
               
                   
                 CCCCUCUGUGUAGCCAGAACCUCGGGGCACCAGGGGGAG 
                   
               
               
                   
                 GUCCUGACAAUGGGCCGCAAGACCCUGACAAUACUGAUG 
                   
               
               
                   
                 AUAAUGGCCCCCAAGAUCCUGACAAUACCGAUGACAACG 
                   
               
               
                   
                 GCCCGCACGACCCCCUGCCGCAGGAUCCGGAUAACACCGA 
                   
               
               
                   
                 CGACAAUGGACCCCAGGACCCCGAUAACACGGACGAUAA 
                   
               
               
                   
                 CGGCCCUCACGAUCCCUUACCCCAUUCUCCGUCCGACUCU 
                   
               
               
                   
                 GCAGGGAACGACGGGGGCCCUCCUCAGUUAACCGAAGAG 
                   
               
               
                   
                 GUCGAAAAUAAGGGGGGAGACCAGGGCCCCCCAUUAAUG 
                   
               
               
                   
                 ACAGACGGGGGCGGCGGUCACAGUCACGACUCAGGUCAC 
                   
               
               
                   
                 GGCGGCGGGGACCCCCAUUUGCCUACACUGCUGUUAGGG 
                   
               
               
                   
                 UCUUCUGGCUCAGGCGGGGACGAUGAUGAUCCACACGGU 
                   
               
               
                   
                 CCGGUGCAGCUGUCAUAUUAUGAU 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEHDLERGPPGPRRPPRGPPLSSSLGLALLLLLLALLFWLYIV 
                  16 
               
               
                 amino acid 
                 MSDWTGGALLVLYSFALMLIIIILIIFIFRRDLLCPLGALCILLLM 
                   
               
               
                 sequence 
                 ITLLLIALWNLHGQALFLGIVLFIFGCLLVLGIWIYLLEMLWRL 
                   
               
               
                   
                 GATIWQLLAFFLAFFLDLILLIIALYLQQNWWTLLVDLLWLLL 
                   
               
               
                   
                 FLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSN 
                   
               
               
                   
                 SNEGRHHLLVSGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTD 
                   
               
               
                   
                 DNGPQDPDNTDDNGPHDPLPQDPDNTDDNGPQDPDNTDDNG 
                   
               
               
                   
                 PHDPLPHSPSDSAGNDGGPPQLTEEVENKGGDQGPPLMTDGG 
                   
               
               
                   
                 GGHSHDSGHGGGDPHLPTLLLGSSGSGGDDDDPHGPVQLSYY 
                   
               
               
                   
                 D 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP2_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 145 consists of from 5′ end to 3′ end: 
                 145 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 21, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGUAGUCUUGAGAUGGUACCAAUGGGCGCAGGUCCA 
                  21 
               
               
                 Construct 
                 CCGAGCCCUGGAGGCGAUCCUGACGGGUACGACGGGGGA 
                   
               
               
                 (excluding 
                 AAUAACUCGCAAUAUCCCUCAGCCUCAGGCUCAUCGGGU 
                   
               
               
                 the stop 
                 AAUACUCCGACCCCUCCAAACGACGAGGAAAGGGAAUCC 
                   
               
               
                 codon) 
                 AAUGAGGAACCGCCUCCACCAUAUGAGGAUCCGUACUGG 
                   
               
               
                   
                 GGGAACGGUGAUAGGCACAGUGACUACCAGCCACUUGGG 
                   
               
               
                   
                 ACACAAGACCAAAGCCUGUACCUCGGACUUCAGCACGAU 
                   
               
               
                   
                 GGUAACGACGGUUUGCCGCCUCCCCCUUAUAGCCCUAGA 
                   
               
               
                   
                 GAUGAUAGCUCCCAGCAUAUAUACGAGGAGGCUGGUAGG 
                   
               
               
                   
                 GGCUCCAUGAAUCCAGUAUGCUUGCCCGUUAUCGUAGCC 
                   
               
               
                   
                 CCCUACCUCUUCUGGCUUGCGGCGAUCGCAGCUAGCUGU 
                   
               
               
                   
                 UUUACAGCUUCCGUGAGCACAGUCGUAACCGCGACAGGA 
                   
               
               
                   
                 CUGGCACUGUCUCUACUCCUCUUGGCAGCGGUUGCCAGC 
                   
               
               
                   
                 AGCUACGCCGCCGCUCAGCGGAAACUGCUCACCCCUGUUA 
                   
               
               
                   
                 CAGUCUUAACUGCAGUUGUGACCUUCUUCGCCAUUUGUU 
                   
               
               
                   
                 UGACAUGGAGGAUCGAAGAUCCUCCAUUUAAUAGUCUCC 
                   
               
               
                   
                 UGUUUGCACUGCUGGCCGCUGCGGGCGGGCUACAAGGGA 
                   
               
               
                   
                 UAUAUGUGCUAGUAAUGCUCGUCCUACUUAUUCUGGCCU 
                   
               
               
                   
                 AUAGGCGGAGGUGGCGGCGCCUGACAGUGUGCGGAGGGA 
                   
               
               
                   
                 UAAUGUUCCUAGCCUGUGUGCUCGUGCUCAUUGUGGACG 
                   
               
               
                   
                 CUGUUCUACAGUUGUCUCCUCUGCUGGGUGCAGUGACGG 
                   
               
               
                   
                 UGGUCUCAAUGACCCUGCUGUUACUUGCCUUCGUGCUUU 
                   
               
               
                   
                 GGUUGUCAUCUCCAGGGGGACUGGGCACACUGGGCGCCG 
                   
               
               
                   
                 CCCUGUUAACCCUGGCGGCCGCUCUGGCACUCCUGGCCUC 
                   
               
               
                   
                 ACUGAUUCUGGGAACUCUGAAUCUCACCACUAUGUUUCU 
                   
               
               
                   
                 UCUGAUGUUAUUGUGGACUUUGGUGGUGUUGCUGAUUUG 
                   
               
               
                   
                 CAGCUCCUGCAGUUCUUGCCCCCUAUCCAAAAUAUUACU 
                   
               
               
                   
                 GGCGCGGCUGUUCCUUUAUGCCCUCGCCUUACUGCUGCUC 
                   
               
               
                   
                 GCUAGCGCCUUAAUUGCUGGGGGCUCCAUUCUUCAGACC 
                   
               
               
                   
                 AACUUUAAAUCCCUGUCCAGCACUGAAUUCAUCCCCAACC 
                   
               
               
                   
                 UUUUUUGCAUGCUGCUCCUAAUCGUGGCUGGGAUUCUGU 
                   
               
               
                   
                 UUAUUCUGGCCAUUUUGACCGAGUGGGGCAGUGGCAACA 
                   
               
               
                   
                 GGACCUACGGCCCUGUGUUCAUGUGUCUGGGGGGGCUCU 
                   
               
               
                   
                 UAACGAUGGUCGCUGGCGCUGUCUGGCUCACGGUGAUGA 
                   
               
               
                   
                 GUAACACUUUGCUCAGCGCGUGGAUCCUGACAGCCGGCU 
                   
               
               
                   
                 UUCUCAUUUUUCUGAUUGGGUUCGCUUUGUUUGGAGUCA 
                   
               
               
                   
                 UUCGGUGUUGCCGCUAUUGCUGUUACUAUUGUUUGACAU 
                   
               
               
                   
                 UGGAGUCCGAGGAGAGGCCACCCACCCCCUACCGAAAUAC 
                   
               
               
                   
                 GGUA 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNT 
                  22 
               
               
                 amino acid 
                 PTPPNDEERESNEEPPPPYEDPYWGNGDRHSDYQPLGTQDQSL 
                   
               
               
                 sequence 
                 YLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLP 
                   
               
               
                   
                 VIVAPYLFWLAAIAASCFTASVSTVVTATGLALSLLLLAAVAS 
                   
               
               
                   
                 SYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLFALL 
                   
               
               
                   
                 AAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVL 
                   
               
               
                   
                 VLIVDAVLQLSPLLGAVTVVSMTLLLLAFVLWLSSPGGLGTL 
                   
               
               
                   
                 GAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICS 
                   
               
               
                   
                 SCSSCPLSKILLARLFLYALALLLLASALIAGGSILQTNFKSLSS 
                   
               
               
                   
                 TEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTYGPVFMCLGG 
                   
               
               
                   
                 LLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGVIRC 
                   
               
               
                   
                 CRYCCYYCLTLESEERPPTPYRNTV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP2_002 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 146 consists of from 5′ end to 3′ end: 
                 146 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 23, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGAUCCUUGGAAAUGGUGCCCAUGGGAGCCGGACCC 
                  23 
               
               
                 Construct 
                 CCCUCACCAGGGGGAGACCCAGACGGAUACGACGGGGGA 
                   
               
               
                 (excluding 
                 AACAAUUCUCAGUAUCCGAGCGCGUCCGGCAGCUCAGGA 
                   
               
               
                 the stop 
                 AACACACCCACCCCCCCCAACGACGAGGAGAGAGAAUCCA 
                   
               
               
                 codon) 
                 ACGAGGAGCCUCCUCCCCCAUACGAAGACCCUUACUGGGG 
                   
               
               
                   
                 GAAUGGAGACCGUCAUUCUGACUAUCAACCUCUAGGCAC 
                   
               
               
                   
                 UCAGGACCAGUCUCUUUACCUAGGGUUACAACACGAUGG 
                   
               
               
                   
                 UAAUGAUGGGUUGCCCCCGCCGCCCUAUAGUCCCCGGGAC 
                   
               
               
                   
                 GACAGUAGUCAGCAUAUAUAUGAGGAAGCCGGCAGAGGU 
                   
               
               
                   
                 UCUAUGAAUCCCGUUUGUCUACCGGUCAUUGUCGCCCCC 
                   
               
               
                   
                 UAUUUAUUCUGGCUUGCAGCCAUCGCCGCUUCGUGCUUC 
                   
               
               
                   
                 ACCGCCUCCGUGUCCACGGUGGUGACAGCUACAGGGCUG 
                   
               
               
                   
                 GCACUCUCUCUGCUACUCCUGGCUGCUGUGGCCAGCUCCU 
                   
               
               
                   
                 ACGCAGCGGCUCAGCGGAAGCUUCUCACUCCUGUAACUG 
                   
               
               
                   
                 UCCUCACCGCGGUAGUGACCUUUUUUGCCAUAUGCCUGA 
                   
               
               
                   
                 CAUGGAGGAUUGAGGACCCACCCUUUAAUUCUCUGCUGU 
                   
               
               
                   
                 UUGCGCUUUUGGCCGCUGCCGGAGGACUGCAAGGUAUUU 
                   
               
               
                   
                 AUGUGCUGGUAAUGUUGGUUCUACUUAUUCUCGCAUACC 
                   
               
               
                   
                 GGCGACGGUGGCGGAGGCUGACUGUAUGCGGGGGGAUUA 
                   
               
               
                   
                 UGUUCCUGGCGUGUGUUCUGGUUUUGAUCGUCGAUGCCG 
                   
               
               
                   
                 UGCUGCAGUUGUCACCGCUGCUCGGGGCGGUCACCGUUG 
                   
               
               
                   
                 UGAGUAUGACACUGCUUCUGCUGGCUUUCGUGCUCUGGC 
                   
               
               
                   
                 UAUCUUCCCCCGGGGGGCUGGGGACCUUGGGCGCUGCGC 
                   
               
               
                   
                 UUCUGACUCUGGCAGCUGCUCUGGCCCUGCUGGCCUCUCU 
                   
               
               
                   
                 UAUUCUUGGCACACUGAAUUUGACUACCAUGUUCCUCUU 
                   
               
               
                   
                 GAUGUUGCUGUGGACCUUAGUUGUGCUGCUGAUCUGUUC 
                   
               
               
                   
                 UUCCUGUAGCAGCUGUCCACUGUCCAAGAUUCUCCUGGC 
                   
               
               
                   
                 CAGGUUGUUUCUGUAUGCCCUAGCGCUGCUGCUGUUAGC 
                   
               
               
                   
                 UUCUGCUCUGAUAGCCGGCGGCAGUAUCCUGCAAACCAA 
                   
               
               
                   
                 CUUCAAGAGCCUCUCCAGCACAGAAUUCAUCCCUAAUCUC 
                   
               
               
                   
                 UUUUGCAUGCUACUGCUCAUCGUGGCAGGCAUACUGUUC 
                   
               
               
                   
                 AUUCUUGCUAUCCUGACUGAAUGGGGCUCUGGCAAUAGA 
                   
               
               
                   
                 ACAUACGGGCCAGUGUUCAUGUGCUUGGGCGGCCUGUUG 
                   
               
               
                   
                 ACAAUGGUUGCUGGAGCCGUGUGGCUGACGGUAAUGUCC 
                   
               
               
                   
                 AACACUCUGCUGAGCGCAUGGAUCUUAACAGCCGGAUUC 
                   
               
               
                   
                 CUGAUCUUUUUAAUCGGAUUCGCACUCUUCGGAGUGAUC 
                   
               
               
                   
                 CGGUGCUGUAGGUAUUGUUGUUAUUACUGCCUGACACUC 
                   
               
               
                   
                 GAGUCGGAAGAGAGGCCCCCUACACCUUACCGGAAUACA 
                   
               
               
                   
                 GUU 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNT 
                  22 
               
               
                 amino acid 
                 PTPPNDEERESNEEPPPPYEDPYWGNGDRHSDYQPLGTQDQSL 
                   
               
               
                 sequence 
                 YLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLP 
                   
               
               
                   
                 VIVAPYLFWLAAIAASCFTASVSTVVTATGLALSLLLLAAVAS 
                   
               
               
                   
                 SYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLFALL 
                   
               
               
                   
                 AAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVL 
                   
               
               
                   
                 VLIVDAVLQLSPLLGAVTVVSMTLLLLAFVLWLSSPGGLGTL 
                   
               
               
                   
                 GAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICS 
                   
               
               
                   
                 SCSSCPLSKILLARLFLYALALLLLASALIAGGSILQTNFKSLSS 
                   
               
               
                   
                 TEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTYGPVFMCLGG 
                   
               
               
                   
                 LLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGVIRC 
                   
               
               
                   
                 CRYCCYYCLTLESEERPPTPYRNTV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP2_003 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 147 consists of from 5′ end to 3′ end: 
                 147 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 24, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGGUCGUUGGAGAUGGUCCCCAUGGGCGCUGGUCCU 
                  24 
               
               
                 Construct 
                 CCAAGCCCCGGCGGGGACCCGGACGGAUACGACGGGGGC 
                   
               
               
                 (excluding 
                 AAUAAUAGUCAGUAUCCGAGUGCAAGCGGAAGCAGUGGG 
                   
               
               
                 the stop 
                 AAUACGCCUACACCACCAAACGACGAGGAGCGGGAGUCU 
                   
               
               
                 codon) 
                 AACGAGGAGCCUCCACCCCCUUACGAGGAUCCUUACUGG 
                   
               
               
                   
                 GGGAACGGAGAUCGACAUUCUGACUACCAGCCUUUAGGG 
                   
               
               
                   
                 ACACAGGAUCAGUCUCUCUAUCUCGGACUGCAGCAUGAU 
                   
               
               
                   
                 GGCAACGACGGACUGCCCCCACCACCUUAUUCUCCACGCG 
                   
               
               
                   
                 ACGACAGCUCACAGCACAUAUACGAAGAGGCCGGGCGCG 
                   
               
               
                   
                 GGUCUAUGAACCCGGUUUGCCUGCCAGUCAUCGUCGCAC 
                   
               
               
                   
                 CCUACCUCUUUUGGCUGGCGGCCAUUGCCGCGUCCUGUU 
                   
               
               
                   
                 UCACAGCCUCAGUGUCGACUGUGGUAACCGCAACAGGGC 
                   
               
               
                   
                 UCGCACUCAGCCUACUGCUGCUCGCUGCCGUCGCGUCCUC 
                   
               
               
                   
                 UUAUGCUGCUGCCCAGCGCAAACUGCUCACCCCAGUUACG 
                   
               
               
                   
                 GUGCUCACUGCUGUUGUUACCUUUUUCGCUAUUUGUCUC 
                   
               
               
                   
                 ACCUGGAGAAUCGAAGACCCGCCGUUCAACAGUCUGUUA 
                   
               
               
                   
                 UUUGCCCUCUUGGCUGCAGCAGGCGGCCUUCAGGGCAUC 
                   
               
               
                   
                 UAUGUCCUGGUGAUGCUGGUCCUGCUCAUCCUGGCUUAC 
                   
               
               
                   
                 AGGAGAAGAUGGCGUCGGCUGACCGUGUGUGGUGGCAUC 
                   
               
               
                   
                 AUGUUUCUCGCGUGUGUUCUGGUGCUCAUCGUCGAUGCC 
                   
               
               
                   
                 GUGCUACAGCUGAGCCCACUGCUUGGAGCCGUGACAGUC 
                   
               
               
                   
                 GUUAGCAUGACACUCCUGCUAUUGGCUUUCGUGCUCUGG 
                   
               
               
                   
                 CUGAGUAGCCCAGGUGGGCUCGGCACACUUGGUGCCGCG 
                   
               
               
                   
                 CUUUUAACACUGGCCGCGGCCCUCGCACUGCUGGCCUCUC 
                   
               
               
                   
                 UAAUUCUCGGAACAUUGAACCUGACAACCAUGUUCCUCC 
                   
               
               
                   
                 UGAUGCUCCUCUGGACACUGGUGGUACUGUUAAUCUGUA 
                   
               
               
                   
                 GCUCUUGCUCAUCAUGCCCACUGAGCAAGAUACUCCUGG 
                   
               
               
                   
                 CGAGACUUUUCCUAUACGCCCUGGCCCUGCUGCUGUUGG 
                   
               
               
                   
                 CAUCGGCUCUGAUCGCUGGGGGAAGCAUCCUUCAAACAA 
                   
               
               
                   
                 AUUUCAAGAGCCUUAGCAGUACUGAGUUUAUACCUAACC 
                   
               
               
                   
                 UGUUCUGUAUGCUUCUGCUGAUAGUCGCUGGGAUACUUU 
                   
               
               
                   
                 UUAUUCUGGCAAUCCUAACAGAGUGGGGGUCAGGUAACA 
                   
               
               
                   
                 GGACCUAUGGGCCUGUUUUUAUGUGCCUCGGAGGCCUCC 
                   
               
               
                   
                 UCACCAUGGUUGCCGGCGCAGUUUGGUUGACAGUGAUGU 
                   
               
               
                   
                 CCAACACCCUACUGAGUGCUUGGAUACUGACGGCCGGGU 
                   
               
               
                   
                 UCCUGAUUUUCCUAAUAGGAUUUGCCCUGUUUGGGGUUA 
                   
               
               
                   
                 UCCGGUGCUGCCGUUACUGCUGCUACUAUUGUUUGACCC 
                   
               
               
                   
                 UUGAGUCUGAAGAAAGGCCGCCCACCCCCUACAGAAACA 
                   
               
               
                   
                 CUGUA 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNT 
                  22 
               
               
                 amino acid 
                 PTPPNDEERESNEEPPPPYEDPYWGNGDRHSDYQPLGTQDQSL 
                   
               
               
                 sequence 
                 YLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLP 
                   
               
               
                   
                 VIVAPYLFWLAAIAASCFTASVSTVVTATGLALSLLLLAAVAS 
                   
               
               
                   
                 SYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLFALL 
                   
               
               
                   
                 AAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVL 
                   
               
               
                   
                 VLIVDAVLQLSPLLGAVTVVSMTLLLLAFVLWLSSPGGLGTL 
                   
               
               
                   
                 GAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICS 
                   
               
               
                   
                 SCSSCPLSKILLARLFLYALALLLLASALIAGGSILQTNFKSLSS 
                   
               
               
                   
                 TEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTYGPVFMCLGG 
                   
               
               
                   
                 LLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGVIRC 
                   
               
               
                   
                 CRYCCYYCLTLESEERPPTPYRNTV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP2_004 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 148 consists of from 5′ end to 3′ end: 
                 148 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 25, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGGUCAUUGGAGAUGGUGCCUAUGGGGGCCGGCCCA 
                  25 
               
               
                 Construct 
                 CCCAGCCCAGGGGGCGAUCCUGACGGAUACGACGGGGGC 
                   
               
               
                 (excluding 
                 AAUAAUUCACAAUAUCCUAGCGCCAGCGGAUCUUCUGGG 
                   
               
               
                 the stop 
                 AAUACCCCCACACCACCUAACGACGAGGAACGGGAGUCA 
                   
               
               
                 codon) 
                 AACGAGGAACCCCCACCGCCGUACGAGGACCCAUACUGGG 
                   
               
               
                   
                 GGAACGGGGAUCGACACUCAGACUACCAGCCUCUCGGAA 
                   
               
               
                   
                 CGCAGGAUCAAAGCCUGUACCUGGGACUGCAGCACGAUG 
                   
               
               
                   
                 GUAACGAUGGACUGCCACCACCUCCUUACAGUCCAAGGG 
                   
               
               
                   
                 AUGACUCCAGCCAGCACAUUUAUGAAGAAGCCGGCCGAG 
                   
               
               
                   
                 GUAGUAUGAAUCCAGUGUGUCUGCCCGUGAUUGUCGCCC 
                   
               
               
                   
                 CUUAUCUCUUUUGGCUGGCCGCCAUUGCCGCCAGCUGUU 
                   
               
               
                   
                 UCACCGCGUCGGUUUCAACCGUAGUGACCGCCACAGGCCU 
                   
               
               
                   
                 GGCUCUCAGCUUGUUACUGUUGGCCGCUGUGGCGAGCUC 
                   
               
               
                   
                 CUACGCCGCCGCCCAGCGCAAGCUACUUACCCCUGUGACA 
                   
               
               
                   
                 GUGCUGACGGCGGUGGUUACAUUCUUCGCCAUUUGCCUG 
                   
               
               
                   
                 ACCUGGCGAAUUGAGGAUCCACCAUUCAAUUCCUUAUUG 
                   
               
               
                   
                 UUUGCCCUCCUCGCCGCUGCUGGAGGGCUCCAGGGUAUC 
                   
               
               
                   
                 UACGUGCUGGUGAUGCUCGUUUUGCUGAUAUUGGCUUAC 
                   
               
               
                   
                 CGGCGGCGCUGGCGCCGCCUUACCGUCUGCGGAGGAAUC 
                   
               
               
                   
                 AUGUUUUUGGCAUGCGUGCUCGUUCUCAUUGUGGAUGCU 
                   
               
               
                   
                 GUUUUGCAGCUGUCACCCCUGUUGGGUGCCGUGACAGUU 
                   
               
               
                   
                 GUAAGUAUGACCCUGCUCCUGCUCGCAUUCGUGCUCUGG 
                   
               
               
                   
                 CUGAGCUCUCCCGGGGGGCUGGGCACUCUCGGAGCAGCU 
                   
               
               
                   
                 CUGCUGACACUAGCCGCAGCACUAGCCCUUCUGGCCAGUC 
                   
               
               
                   
                 UUAUCCUCGGAACGCUGAACCUGACGACCAUGUUCCUCC 
                   
               
               
                   
                 UGAUGUUGCUCUGGACCCUCGUAGUGCUGUUAAUAUGCU 
                   
               
               
                   
                 CUAGCUGUAGCUCCUGCCCUCUGAGCAAGAUUCUCCUUG 
                   
               
               
                   
                 CUAGGCUGUUCCUAUACGCCCUCGCUCUCCUAUUGCUGGC 
                   
               
               
                   
                 GAGCGCUCUCAUCGCCGGUGGGAGCAUCCUGCAGACUAA 
                   
               
               
                   
                 CUUCAAGUCCCUGAGUUCAACAGAAUUCAUUCCCAACCU 
                   
               
               
                   
                 CUUUUGUAUGCUGCUGCUUAUCGUGGCCGGCAUCCUCUU 
                   
               
               
                   
                 UAUCCUCGCCAUCUUGACUGAGUGGGGGUCCGGAAAUCG 
                   
               
               
                   
                 CACAUAUGGCCCAGUUUUCAUGUGUCUGGGGGGCCUGCU 
                   
               
               
                   
                 CACGAUGGUGGCCGGCGCAGUGUGGCUGACGGUAAUGUC 
                   
               
               
                   
                 AAACACUUUGCUCUCUGCGUGGAUCUUGACCGCAGGCUU 
                   
               
               
                   
                 CCUGAUCUUUUUGAUCGGCUUCGCCCUGUUCGGUGUCAU 
                   
               
               
                   
                 CCGGUGCUGCAGAUACUGCUGCUACUAUUGCCUCACACU 
                   
               
               
                   
                 CGAAUCCGAAGAGAGGCCUCCCACACCUUACCGAAAUAC 
                   
               
               
                   
                 AGUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNT 
                  22 
               
               
                 amino acid 
                 PTPPNDEERESNEEPPPPYEDPYWGNGDRHSDYQPLGTQDQSL 
                   
               
               
                 sequence 
                 YLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLP 
                   
               
               
                   
                 VIVAPYLFWLAAIAASCFTASVSTVVTATGLALSLLLLAAVAS 
                   
               
               
                   
                 SYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLFALL 
                   
               
               
                   
                 AAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVL 
                   
               
               
                   
                 VLIVDAVLQLSPLLGAVTVVSMTLLLLAFVLWLSSPGGLGTL 
                   
               
               
                   
                 GAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICS 
                   
               
               
                   
                 SCSSCPLSKILLARLFLYALALLLLASALIAGGSILQTNFKSLSS 
                   
               
               
                   
                 TEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTYGPVFMCLGG 
                   
               
               
                   
                 LLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGVIRC 
                   
               
               
                   
                 CRYCCYYCLTLESEERPPTPYRNTV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP2_005 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 149 consists of from 5′ end to 3′ end: 
                 149 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 26, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGGUCUUUAGAAAUGGUCCCCAUGGGUGCCGGCCCA 
                  26 
               
               
                 Construct 
                 CCCUCGCCAGGAGGCGAUCCAGACGGCUACGACGGGGGA 
                   
               
               
                 (excluding 
                 AACAACAGCCAGUACCCCAGCGCCAGCGGUAGCAGCGGCA 
                   
               
               
                 the stop 
                 ACACCCCGACCCCACCAAACGACGAAGAGCGGGAGAGCAA 
                   
               
               
                 codon) 
                 UGAGGAGCCCCCACCCCCCUAUGAGGACCCUUACUGGGGA 
                   
               
               
                   
                 AAUGGCGAUCGACACAGCGAUUACCAGCCCCUGGGCACA 
                   
               
               
                   
                 CAGGAUCAGAGCCUGUACCUGGGCUUGCAGCAUGAUGGU 
                   
               
               
                   
                 AACGACGGGCUUCCGCCCCCACCAUACUCACCCAGAGACG 
                   
               
               
                   
                 AUUCAAGUCAGCACAUCUACGAGGAGGCUGGUAGGGGUA 
                   
               
               
                   
                 GUAUGAAUCCUGUGUGUCUCCCUGUGAUCGUCGCCCCCU 
                   
               
               
                   
                 ACCUAUUUUGGUUAGCUGCGAUCGCUGCCUCCUGCUUCA 
                   
               
               
                   
                 CCGCUAGCGUGUCAACAGUGGUUACGGCAACCGGGCUGG 
                   
               
               
                   
                 CGUUAAGUCUGCUUCUGCUUGCGGCAGUGGCAUCUUCUU 
                   
               
               
                   
                 AUGCAGCGGCCCAGCGGAAGCUUUUGACCCCUGUGACAG 
                   
               
               
                   
                 UACUGACCGCAGUGGUUACGUUUUUUGCGAUUUGCUUGA 
                   
               
               
                   
                 CGUGGAGAAUAGAGGAUCCCCCUUUUAACUCACUGCUCU 
                   
               
               
                   
                 UUGCACUGCUCGCGGCUGCUGGGGGCCUGCAGGGGAUUU 
                   
               
               
                   
                 ACGUCCUCGUUAUGCUGGUGCUCCUGAUCUUAGCCUACA 
                   
               
               
                   
                 GAAGGAGAUGGCGCCGACUGACAGUAUGCGGGGGGAUUA 
                   
               
               
                   
                 UGUUUUUGGCAUGUGUACUCGUAUUGAUCGUGGAUGCCG 
                   
               
               
                   
                 UCUUGCAGCUUUCCCCCCUGCUGGGCGCAGUCACCGUCGU 
                   
               
               
                   
                 GAGUAUGACUCUGCUGUUACUGGCCUUCGUACUGUGGCU 
                   
               
               
                   
                 UAGCAGCCCUGGAGGACUAGGCACGCUGGGCGCCGCUUU 
                   
               
               
                   
                 AUUGACCCUGGCAGCCGCCCUGGCUCUGCUGGCCUCCCUG 
                   
               
               
                   
                 AUACUGGGGACAUUAAAUUUAACAACUAUGUUUCUGCUG 
                   
               
               
                   
                 AUGCUGCUGUGGACUCUAGUUGUGCUCUUGAUAUGUUCC 
                   
               
               
                   
                 UCCUGUUCCAGCUGCCCUCUGAGCAAGAUCCUGUUGGCU 
                   
               
               
                   
                 CGGCUAUUCCUGUACGCGCUGGCUCUCCUCCUGCUAGCUU 
                   
               
               
                   
                 CAGCGCUAAUUGCAGGCGGGUCUAUUCUCCAGACGAAUU 
                   
               
               
                   
                 UUAAGAGCCUCAGUAGCACUGAGUUUAUCCCGAAUCUGU 
                   
               
               
                   
                 UCUGCAUGUUGCUAUUGAUCGUCGCAGGCAUUCUCUUCA 
                   
               
               
                   
                 UUCUGGCCAUACUUACAGAGUGGGGUAGCGGCAACCGGA 
                   
               
               
                   
                 CUUAUGGCCCUGUGUUCAUGUGCUUGGGGGGCCUUCUCA 
                   
               
               
                   
                 CAAUGGUGGCAGGGGCGGUAUGGCUGACCGUGAUGUCUA 
                   
               
               
                   
                 ACACCUUGUUAUCAGCCUGGAUCCUUACCGCCGGCUUCCU 
                   
               
               
                   
                 AAUUUUUCUGAUCGGCUUCGCCCUCUUCGGAGUAAUACG 
                   
               
               
                   
                 AUGCUGUCGGUACUGCUGCUACUAUUGCCUUACUUUGGA 
                   
               
               
                   
                 GAGCGAAGAGCGGCCUCCCACUCCCUAUAGAAACACCGU 
                   
               
               
                   
                 U 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNT 
                  22 
               
               
                 amino acid 
                 PTPPNDEERESNEEPPPPYEDPYWGNGDRHSDYQPLGTQDQSL 
                   
               
               
                 sequence 
                 YLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLP 
                   
               
               
                   
                 VIVAPYLFWLAAIAASCFTASVSTVVTATGLALSLLLLAAVAS 
                   
               
               
                   
                 SYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLFALL 
                   
               
               
                   
                 AAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVL 
                   
               
               
                   
                 VLIVDAVLQLSPLLGAVTVVSMTLLLLAFVLWLSSPGGLGTL 
                   
               
               
                   
                 GAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICS 
                   
               
               
                   
                 SCSSCPLSKILLARLFLYALALLLLASALIAGGSILQTNFKSLSS 
                   
               
               
                   
                 TEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTYGPVFMCLGG 
                   
               
               
                   
                 LLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGVIRC 
                   
               
               
                   
                 CRYCCYYCLTLESEERPPTPYRNTV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 gp350_mod_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 150 consists of from 5′ end to 3′ end: 
                 150 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 27, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  27 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UUUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUU 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACCCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUUUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAAACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACCCCCGCAGGUACAACCUCAGGCG 
                   
               
               
                   
                 CCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUGG 
                   
               
               
                   
                 AACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCAG 
                   
               
               
                   
                 CCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAACC 
                   
               
               
                   
                 CCAGCUGUGACGACCCCCACCCCGAACGCGACAUCUCCCA 
                   
               
               
                   
                 CACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCCC 
                   
               
               
                   
                 UACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAAC 
                   
               
               
                   
                 CCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGACU 
                   
               
               
                   
                 UCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGGG 
                   
               
               
                   
                 ACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAACG 
                   
               
               
                   
                 CUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCAG 
                   
               
               
                   
                 CUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAAA 
                   
               
               
                   
                 UCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUACU 
                   
               
               
                   
                 UCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGCG 
                   
               
               
                   
                 GCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCCA 
                   
               
               
                   
                 CCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAGG 
                   
               
               
                   
                 GACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUAC 
                   
               
               
                   
                 AAGCACAAAACCAGGCGAAGUUAACGUGACAAAAGGAAC 
                   
               
               
                   
                 GCCCCCCCAGAACGCAACCAGUCCUCAGGCCCCCAGCGGG 
                   
               
               
                   
                 CAGAAAACUGCGGUGCCAACUGUGACCAGCACCGGUGGC 
                   
               
               
                   
                 AAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGGG 
                   
               
               
                   
                 CACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUACG 
                   
               
               
                   
                 GAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCCA 
                   
               
               
                   
                 CCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGCC 
                   
               
               
                   
                 ACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCAA 
                   
               
               
                   
                 GCGACGGUGCCAGUGCCCCCUACAAGCCAGCCACGCUUCU 
                   
               
               
                   
                 CCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUUG 
                   
               
               
                   
                 CUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAUU 
                   
               
               
                   
                 GCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUACA 
                   
               
               
                   
                 CCACGCCCCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  28 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 gp350_mod_002 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 151 consists of from 5′ end to 3′ end: 
                 151 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 29, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCUGCUCUAUUGGUGUGCCAAUACACCAUUCAG 
                  29 
               
               
                 Construct 
                 UCGCUGAUCCACUUGACUGGGGAGGAUCCCGGAUUUUUC 
                   
               
               
                 (excluding 
                 AACGUGGAGAUUCCGGAAUUCCCUUUUUACCCCACCUGC 
                   
               
               
                 the stop 
                 AACGUGUGCACUGCCGACGUAAACGUGACCAUAAACUUU 
                   
               
               
                 codon) 
                 GACGUCGGUGGGAAGAAGCACCAGCUGGACCUGGACUUC 
                   
               
               
                   
                 GGGCAGUUAACACCCCACACAAAGGCAGUUUACCAGCCC 
                   
               
               
                   
                 AGAGGAGCCUUCGGCGGAUCGGAGAAUGCAACAAAUCUU 
                   
               
               
                   
                 UUCCUGCUAGAACUGCUCGGCGCCGGCGAGUUGGCCCUG 
                   
               
               
                   
                 ACAAUGAGAAGCAAGAAACUUCCUAUCAACGUAACCACA 
                   
               
               
                   
                 GGGGAGGAGCAGCAGGUCAGCCUCGAAAGUGUUGACGUC 
                   
               
               
                   
                 UAUUUCCAGGAUGUCUUUGGGACUAUGUGGUGCCAUCAC 
                   
               
               
                   
                 GCCGAAAUGCAGAAUCCCGUGUACCUCAUUCCCGAGACA 
                   
               
               
                   
                 GUACCCUAUAUCAAGUGGGACAACUGCAACUCUACUAAU 
                   
               
               
                   
                 AUUACAGCCGUGGUGCGCGCUCAGGGACUUGAUGUAACA 
                   
               
               
                   
                 UUGCCUUUGUCUUUGCCCACAAGCGCUCAGGAUUCUAAU 
                   
               
               
                   
                 UUCUCUGUAAAGACGGAGAUGCUGGGGAAUGAAAUCGAC 
                   
               
               
                   
                 AUAGAAUGUAUUAUGGAAGACGGUGAAAUCAGUCAGGUG 
                   
               
               
                   
                 CUACCGGGAGACAACAAAUUCAAUAUUACAUGUUCUGGC 
                   
               
               
                   
                 UAUGAGAGCCACGUGCCGAGCGGGGGCAUCCUGACUUCG 
                   
               
               
                   
                 ACUUCUCCCGUCGCCACACCCAUACCAGGCACAGGCUACG 
                   
               
               
                   
                 CUUAUAGUCUGAGGCUGACUCCCAGGCCAGUAUCCCGAU 
                   
               
               
                   
                 UUCUGGGUAAUAAUUCCAUCCUUUAUGUGUUUUACUCCG 
                   
               
               
                   
                 GUAACGGCCCCAAAGCUUCGGGGGGCGACUAUUGUAUCC 
                   
               
               
                   
                 AGUCUAAUAUAGUGUUCUCCGAUGAGAUUCCCGCGAGUC 
                   
               
               
                   
                 AGGAUAUGCCCACCAACACCACCGACAUCACGUAUGUUG 
                   
               
               
                   
                 GGGAUAACGCUACCUAUAGUGUGCCGAUGGUGACAUCCG 
                   
               
               
                   
                 AGGACGCAAACUCUCCUAACGUAACCGUGACCGCCUUUU 
                   
               
               
                   
                 GGGCAUGGCCAAAUAAUACCGAAACCGAUUUUAAAUGCA 
                   
               
               
                   
                 AGUGGACCCUGACAUCCGGGACUCCCUCCGGAUGCGAAA 
                   
               
               
                   
                 AUAUUUCUGGAGCUUUUGCCAGCAAUCGGACCUUUGAUA 
                   
               
               
                   
                 UCACUGUGAGUGGUUUAGGCACCGCCCCAAAAACACUGA 
                   
               
               
                   
                 UCAUAACCAGGACAGCCACAAAUGCGACCACAACUACAC 
                   
               
               
                   
                 ACAAAGUUAUAUUUAGUAAAGCACCUGAGUCCACCACAA 
                   
               
               
                   
                 CUUCUCCGACCCUCAACACUACCGGCUUCGCUGAUCCGAA 
                   
               
               
                   
                 UACCACCACAGGUCUUCCAUCUUCCACCCACGUUCCAACU 
                   
               
               
                   
                 AAUCUUACGGCGCCUGCUAGCACCGGUCCAACCGUGUCCA 
                   
               
               
                   
                 CCGCCGAUGUGACUAGCCCCACGCCGGCUGGAACAACAUC 
                   
               
               
                   
                 AGGGGCCAGCCCUGUAACCCCAAGCCCUUCACCCUGGGAC 
                   
               
               
                   
                 AAUGGUACCGAGAGUAAGGCGCCCGACAUGACCUCAUCA 
                   
               
               
                   
                 ACUUCUCCUGUUACAACUCCAACACCAAAUGCCACUAGUC 
                   
               
               
                   
                 CUACCCCCGCAGUCACUACCCCAACGCCUAAUGCAACGUC 
                   
               
               
                   
                 ACCAACUCCUGCAGUGACCACACCAACACCCAAUGCUACU 
                   
               
               
                   
                 UCGCCGACCCUGGGCAAGACCAGCCCAACCAGUGCCGUGA 
                   
               
               
                   
                 CGACACCGACUCCAAAUGCGACCGGACCUACUGUCGGUG 
                   
               
               
                   
                 AGACAAGCCCCCAGGCAAACGCGACCAACCACACCCUCGG 
                   
               
               
                   
                 CGGGACUUCUCCAACACCCGUCGUAACGUCCCAACCAAAA 
                   
               
               
                   
                 AAUGCUACAUCGGCCGUGACUACCGGACAGCAUAAUAUC 
                   
               
               
                   
                 ACCUCAAGCUCCACGUCGUCAAUGAGCCUGCGGCCGUCAA 
                   
               
               
                   
                 GUAAUCCAGAGACUCUGUCGCCAUCUACCUCAGAUAACU 
                   
               
               
                   
                 CAACUAGCCACAUGCCUCUACUCACUAGUGCCCACCCCAC 
                   
               
               
                   
                 CGGCGGGGAGAACAUCACGCAGGUAACACCAGCGUCUAU 
                   
               
               
                   
                 CAGUACUCACCAUGUGAGUACCAGCUCCCCCGCUCCUCGA 
                   
               
               
                   
                 CCGGGAACCACGAGCCAGGCCAGCGGACCGGGUAAUAGU 
                   
               
               
                   
                 AGCACUUCUACUAAACCGGGAGAGGUCAAUGUGACUAAG 
                   
               
               
                   
                 GGGACUCCCCCCCAAAACGCUACCAGCCCACAGGCUCCCU 
                   
               
               
                   
                 CAGGGCAGAAGACCGCUGUUCCAACAGUUACAUCUACUG 
                   
               
               
                   
                 GGGGCAAGGCAAAUAGCACAACCGGUGGCAAGCAUACUA 
                   
               
               
                   
                 CUGGGCACGGUGCCCGCACAAGCACCGAGCCUACAACGGA 
                   
               
               
                   
                 CUAUGGGGGGGAUUCUACCACACCACGUCCCCGGUAUAA 
                   
               
               
                   
                 CGCCACCACGUAUCUUCCGCCUAGCACAUCAAGCAAACUC 
                   
               
               
                   
                 AGACCUCGCUGGACAUUUACAUCACCCCCAGUUACCACUG 
                   
               
               
                   
                 CUCAGGCAACCGUCCCGGUGCCUCCUACUUCACAACCACG 
                   
               
               
                   
                 UUUCAGCAACUUGAGCAUGCUGGUCCUGCAGUGGGCUUC 
                   
               
               
                   
                 CCUCGCCGUGCUGACAUUACUGUUAUUGCUGGUGAUGGC 
                   
               
               
                   
                 CGAUUGCGCGUUUCGCAGGAACCUGUCUACCAGCCACAC 
                   
               
               
                   
                 AUACACAACGCCCCCGUAUGACGACGCAGAGACUUAUGU 
                   
               
               
                   
                 G 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  28 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 gp350_mod_003 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 152 consists of from 5′ end to 3′ end: 
                 152 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 30, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAAGCCGCUCUUCUGGUCUGCCAGUAUACAAUCCAG 
                  30 
               
               
                 Construct 
                 UCGCUGAUUCAUCUCACCGGCGAGGAUCCAGGUUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAGAUUCCAGAAUUCCCUUUCUAUCCAACUUGU 
                   
               
               
                 the stop 
                 AACGUGUGUACUGCCGACGUUAACGUUACUAUUAACUUU 
                   
               
               
                 codon) 
                 GACGUGGGCGGAAAGAAGCACCAGCUGGAUCUUGAUUUC 
                   
               
               
                   
                 GGCCAGCUAACACCUCAUACCAAGGCCGUCUACCAACCAC 
                   
               
               
                   
                 GCGGGGCAUUUGGCGGUUCUGAGAAUGCUACAAACCUGU 
                   
               
               
                   
                 UCCUUUUGGAACUCCUCGGAGCAGGGGAACUGGCACUUA 
                   
               
               
                   
                 CAAUGAGGAGUAAGAAGCUGCCCAUCAACGUCACCACGG 
                   
               
               
                   
                 GGGAAGAACAGCAGGUGUCCCUGGAGUCCGUUGAUGUGU 
                   
               
               
                   
                 AUUUCCAGGACGUUUUCGGGACAAUGUGGUGUCAUCAUG 
                   
               
               
                   
                 CCGAAAUGCAGAAUCCGGUGUAUCUCAUUCCUGAGACCG 
                   
               
               
                   
                 UGCCCUAUAUUAAAUGGGAUAACUGUAAUUCGACAAACA 
                   
               
               
                   
                 UAACUGCCGUAGUGCGUGCCCAGGGUCUGGAUGUAACUU 
                   
               
               
                   
                 UGCCUCUCUCCCUUCCCACUUCUGCCCAGGACUCCAAUUU 
                   
               
               
                   
                 UUCAGUGAAGACCGAAAUGCUUGGGAACGAGAUCGACAU 
                   
               
               
                   
                 CGAGUGUAUCAUGGAGGAUGGGGAGAUCAGUCAGGUGUU 
                   
               
               
                   
                 GCCUGGUGACAAUAAGUUUAAUAUAACAUGUUCCGGCUA 
                   
               
               
                   
                 UGAAUCACAUGUUCCAAGCGGAGGUAUUCUCACAAGUAC 
                   
               
               
                   
                 AUCUCCCGUAGCCACGCCGAUUCCGGGAACCGGAUACGCC 
                   
               
               
                   
                 UAUUCACUUCGGCUGACUCCUCGGCCAGUGUCACGAUUC 
                   
               
               
                   
                 CUGGGCAAUAACUCAAUUCUGUACGUCUUUUACAGCGGC 
                   
               
               
                   
                 AACGGCCCAAAGGCCAGUGGAGGCGAUUACUGUAUCCAG 
                   
               
               
                   
                 UCUAACAUCGUAUUUUCAGAUGAGAUUCCAGCAAGCCAA 
                   
               
               
                   
                 GACAUGCCAACUAAUACUACAGACAUUACAUACGUCGGU 
                   
               
               
                   
                 GAUAACGCCACUUAUUCAGUCCCUAUGGUCACAUCCGAG 
                   
               
               
                   
                 GACGCGAACUCACCCAAUGUUACCGUUACCGCAUUCUGG 
                   
               
               
                   
                 GCAUGGCCCAAUAAUACGGAAACAGACUUCAAAUGUAAG 
                   
               
               
                   
                 UGGACCCUGACCAGUGGCACCCCUUCUGGCUGCGAAAAU 
                   
               
               
                   
                 AUAAGCGGGGCAUUUGCUAGCAACAGAACAUUCGAUAUU 
                   
               
               
                   
                 ACUGUGUCUGGCCUGGGGACCGCCCCCAAAACACUCAUU 
                   
               
               
                   
                 AUAACGCGAACAGCUACGAACGCUACCACAACCACACAU 
                   
               
               
                   
                 AAAGUGAUUUUCAGCAAGGCUCCAGAAUCAACUACAACC 
                   
               
               
                   
                 AGUCCGACCCUUAAUACCACAGGCUUCGCAGACCCGAACA 
                   
               
               
                   
                 CAACGACGGGACUGCCCUCCUCAACGCAUGUGCCGACUAA 
                   
               
               
                   
                 UCUGACUGCUCCCGCAAGUACCGGCCCAACAGUCUCCACA 
                   
               
               
                   
                 GCAGACGUCACCUCUCCGACUCCCGCCGGCACUACAUCUG 
                   
               
               
                   
                 GAGCCUCACCGGUGACUCCUAGUCCUUCCCCCUGGGACAA 
                   
               
               
                   
                 UGGGACCGAGUCUAAAGCCCCGGACAUGACGAGUUCUAC 
                   
               
               
                   
                 CUCACCAGUUACAACCCCAACACCUAAUGCCACAAGCCCU 
                   
               
               
                   
                 ACGCCAGCCGUGACCACCCCUACCCCCAAUGCCACCAGCC 
                   
               
               
                   
                 CAACACCCGCUGUUACAACGCCAACCCCCAACGCUACUUC 
                   
               
               
                   
                 UCCAACGCUAGGAAAAACAUCACCCACCUCAGCUGUAACC 
                   
               
               
                   
                 ACCCCCACACCAAACGCUACCGGGCCAACCGUUGGCGAAA 
                   
               
               
                   
                 CCUCCCCCCAGGCCAAUGCGACAAACCACACCCUUGGGGG 
                   
               
               
                   
                 GACUUCUCCCACUCCUGUGGUGACUUCACAACCGAAGAA 
                   
               
               
                   
                 CGCGACGUCGGCCGUUACGACCGGGCAGCACAAUAUCACC 
                   
               
               
                   
                 UCGUCCUCCACGUCGUCCAUGUCCUUGCGGCCUUCCAGUA 
                   
               
               
                   
                 ACCCAGAGACACUGUCUCCUAGUACCAGUGACAACAGCA 
                   
               
               
                   
                 CCUCUCACAUGCCAUUACUGACUUCCGCCCACCCAACCGG 
                   
               
               
                   
                 CGGAGAGAACAUCACUCAGGUGACCCCCGCAUCUAUUUC 
                   
               
               
                   
                 UACCCACCACGUGAGCACUAGCAGCCCGGCACCAAGACCA 
                   
               
               
                   
                 GGGACCACCUCCCAGGCUAGCGGCCCGGGCAACUCCUCCA 
                   
               
               
                   
                 CUAGCACGAAACCAGGAGAGGUGAACGUGACCAAGGGCA 
                   
               
               
                   
                 CACCGCCACAGAAUGCAACUUCCCCACAGGCCCCCUCCGG 
                   
               
               
                   
                 CCAGAAAACUGCUGUACCUACGGUCACUAGCACUGGCGG 
                   
               
               
                   
                 AAAAGCGAAUUCAACCACCGGUGGUAAGCAUACCACCGG 
                   
               
               
                   
                 ACACGGCGCUCGGACUAGCACAGAGCCAACCACUGAUUA 
                   
               
               
                   
                 CGGGGGUGACUCAACGACCCCGAGGCCCAGGUACAACGC 
                   
               
               
                   
                 AACCACCUAUCUCCCACCAUCCACAUCUUCCAAGCUCAGG 
                   
               
               
                   
                 CCUCGUUGGACUUUCACCUCCCCGCCUGUGACUACAGCGC 
                   
               
               
                   
                 AGGCCACAGUGCCAGUGCCUCCAACGUCACAGCCGCGGUU 
                   
               
               
                   
                 UAGCAAUCUCUCCAUGCUGGUCCUGCAGUGGGCAUCUCU 
                   
               
               
                   
                 GGCAGUCCUGACACUGCUUCUUUUGCUCGUGAUGGCCGA 
                   
               
               
                   
                 UUGUGCGUUUAGACGCAACCUUUCAACAUCCCACACAUA 
                   
               
               
                   
                 UACAACUCCACCCUAUGAUGACGCAGAGACUUAUGUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  28 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 gp350_mod_004 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 153 consists of from 5′ end to 3′ end: 
                 153 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 31, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAAGCCGCCCUAUUAGUCUGCCAGUACACCAUUCAA 
                  31 
               
               
                 Construct 
                 AGCCUCAUCCACUUGACUGGGGAGGAUCCUGGGUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCGGAAUUCCCUUUCUACCCAACUUGC 
                   
               
               
                 the stop 
                 AACGUGUGCACGGCUGACGUCAACGUGACUAUCAACUUC 
                   
               
               
                 codon) 
                 GAUGUGGGGGGGAAGAAACAUCAACUUGAUCUGGACUUU 
                   
               
               
                   
                 GGCCAGCUUACUCCCCACACCAAGGCCGUGUACCAGCCCC 
                   
               
               
                   
                 GCGGCGCAUUCGGUGGGAGCGAGAACGCGACAAAUCUAU 
                   
               
               
                   
                 UCUUGCUGGAACUGCUGGGCGCAGGAGAGCUCGCCCUGA 
                   
               
               
                   
                 CGAUGCGAUCUAAAAAGCUUCCCAUCAACGUUACCACUG 
                   
               
               
                   
                 GCGAGGAGCAGCAGGUGUCCCUCGAAAGCGUCGACGUCU 
                   
               
               
                   
                 AUUUCCAAGACGUCUUCGGAACAAUGUGGUGCCACCACG 
                   
               
               
                   
                 CUGAGAUGCAGAACCCCGUAUAUCUUAUUCCCGAAACCG 
                   
               
               
                   
                 UGCCAUACAUCAAGUGGGACAACUGCAACUCAACUAACA 
                   
               
               
                   
                 UCACUGCAGUAGUGCGCGCCCAGGGACUGGACGUCACUU 
                   
               
               
                   
                 UGCCACUGUCACUGCCCACAUCAGCCCAAGACAGCAACUU 
                   
               
               
                   
                 CAGCGUCAAGACAGAAAUGCUGGGCAACGAAAUCGACAU 
                   
               
               
                   
                 CGAAUGCAUAAUGGAGGAUGGGGAAAUUUCCCAGGUCCU 
                   
               
               
                   
                 CCCCGGUGAUAACAAGUUUAAUAUUACUUGUUCUGGAUA 
                   
               
               
                   
                 UGAAUCCCAUGUGCCGUCUGGUGGUAUACUGACGAGUAC 
                   
               
               
                   
                 AUCGCCAGUGGCUACCCCAAUCCCCGGGACAGGCUAUGCC 
                   
               
               
                   
                 UACAGCCUGAGACUGACGCCAAGACCAGUUUCCAGGUUU 
                   
               
               
                   
                 CUGGGGAAUAACUCUAUCCUGUAUGUGUUUUAUUCUGGU 
                   
               
               
                   
                 AACGGACCCAAGGCAUCCGGCGGUGAUUAUUGUAUCCAG 
                   
               
               
                   
                 AGCAAUAUAGUGUUCUCUGAUGAGAUUCCGGCCUCCCAG 
                   
               
               
                   
                 GAUAUGCCAACCAACACGACAGAUAUCACCUAUGUCGGG 
                   
               
               
                   
                 GACAACGCCACAUAUAGCGUGCCAAUGGUGACCAGCGAA 
                   
               
               
                   
                 GAUGCCAAUUCUCCCAACGUCACAGUGACAGCCUUUUGG 
                   
               
               
                   
                 GCGUGGCCUAAUAACACAGAAACUGACUUUAAAUGCAAA 
                   
               
               
                   
                 UGGACUCUAACCUCAGGCACCCCAUCUGGCUGCGAGAAC 
                   
               
               
                   
                 AUAAGCGGCGCGUUUGCAUCGAACAGAACUUUUGAUAUU 
                   
               
               
                   
                 ACAGUAUCUGGGUUGGGCACUGCCCCCAAAACUCUAAUA 
                   
               
               
                   
                 AUUACACGGACGGCCACUAACGCCACCACGACGACUCACA 
                   
               
               
                   
                 AGGUGAUCUUCAGCAAAGCUCCCGAAUCUACUACUACCU 
                   
               
               
                   
                 CGCCGACACUGAAUACGACAGGAUUCGCCGACCCAAAUA 
                   
               
               
                   
                 CUACGACCGGCCUUCCCAGCUCGACACAUGUGCCUACAAA 
                   
               
               
                   
                 CUUAACGGCCCCCGCCAGUACCGGCCCCACCGUGAGCACC 
                   
               
               
                   
                 GCUGACGUCACCUCUCCUACCCCUGCCGGGACUACAAGUG 
                   
               
               
                   
                 GUGCUUCCCCAGUUACUCCCAGCCCUAGCCCUUGGGACAA 
                   
               
               
                   
                 CGGCACUGAGAGCAAAGCUCCGGACAUGACCAGCAGUAC 
                   
               
               
                   
                 CAGUCCAGUGACAACUCCGACACCCAAUGCGACGUCCCCA 
                   
               
               
                   
                 ACUCCCGCCGUGACCACGCCAACACCAAAUGCCACGUCAC 
                   
               
               
                   
                 CUACUCCAGCGGUCACCACCCCAACGCCAAACGCUACUAG 
                   
               
               
                   
                 UCCCACUCUAGGCAAGACAUCUCCCACCUCCGCUGUCACC 
                   
               
               
                   
                 ACCCCUACACCCAAUGCUACCGGUCCCACGGUGGGUGAGA 
                   
               
               
                   
                 CUAGCCCACAGGCCAACGCAACUAAUCAUACAUUGGGGG 
                   
               
               
                   
                 GCACAAGCCCAACCCCCGUCGUCACUAGCCAGCCUAAAAA 
                   
               
               
                   
                 CGCCACAAGCGCUGUAACUACCGGCCAGCACAAUAUAAC 
                   
               
               
                   
                 AAGUUCCAGUACUAGCAGUAUGUCUCUGAGACCCAGCUC 
                   
               
               
                   
                 AAAUCCUGAAACCCUCUCCCCCUCUACGUCGGAUAACUCC 
                   
               
               
                   
                 ACGUCCCACAUGCCUCUACUCACAAGCGCGCACCCUACCG 
                   
               
               
                   
                 GGGGAGAAAAUAUCACUCAGGUUACCCCAGCUUCUAUCU 
                   
               
               
                   
                 CGACUCACCAUGUCUCUACUUCUUCCCCAGCGCCACGGCC 
                   
               
               
                   
                 CGGCACUACCAGCCAGGCUAGCGGGCCAGGUAAUUCCUCC 
                   
               
               
                   
                 ACUAGCACUAAACCUGGCGAAGUCAACGUUACCAAGGGA 
                   
               
               
                   
                 ACACCCCCCCAGAAUGCAACCUCCCCCCAAGCUCCCUCUG 
                   
               
               
                   
                 GACAGAAAACAGCCGUCCCUACCGUCACAAGCACCGGUG 
                   
               
               
                   
                 GGAAGGCGAAUUCUACGACAGGCGGGAAGCACACUACCG 
                   
               
               
                   
                 GACACGGAGCAAGGACAUCCACUGAGCCCACGACCGACU 
                   
               
               
                   
                 ACGGUGGCGACUCCACCACCCCCAGACCCAGGUACAAUGC 
                   
               
               
                   
                 GACUACCUACCUGCCCCCUUCCACUUCCAGCAAACUGAGG 
                   
               
               
                   
                 CCUCGUUGGACUUUCACUUCUCCCCCAGUCACCACCGCUC 
                   
               
               
                   
                 AGGCUACCGUGCCUGUCCCGCCGACCUCUCAGCCCCGGUU 
                   
               
               
                   
                 UUCAAAUUUGAGUAUGCUCGUGCUGCAGUGGGCCAGCCU 
                   
               
               
                   
                 GGCAGUGCUUACCCUCCUGCUCUUGCUGGUUAUGGCUGA 
                   
               
               
                   
                 CUGCGCCUUCCGACGUAACUUAUCCACCAGUCAUACUUAC 
                   
               
               
                   
                 ACUACACCCCCUUAUGACGACGCGGAGACCUACGUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  28 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 gp350_mod_005 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 154 consists of from 5′ end to 3′ end: 
                 154 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 32, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAAGCCGCCUUACUGGUCUGCCAGUACACUAUUCAG 
                  32 
               
               
                 Construct 
                 UCCCUCAUCCAUCUCACAGGCGAGGACCCGGGGUUCUUCA 
                   
               
               
                 (excluding 
                 ACGUCGAAAUCCCCGAAUUCCCAUUCUAUCCCACCUGUAA 
                   
               
               
                 the stop 
                 CGUCUGCACAGCCGACGUUAACGUUACAAUCAAUUUCGA 
                   
               
               
                 codon) 
                 CGUGGGCGGGAAAAAACACCAGCUGGACCUCGACUUUGG 
                   
               
               
                   
                 GCAGCUGACGCCCCACACUAAAGCCGUUUACCAGCCCAGG 
                   
               
               
                   
                 GGUGCAUUCGGUGGAUCUGAAAAUGCUACAAACCUCUUU 
                   
               
               
                   
                 CUGCUUGAGCUGCUUGGUGCUGGAGAGCUGGCCCUGACA 
                   
               
               
                   
                 AUGCGCAGCAAAAAGUUGCCAAUCAAUGUGACCACAGGU 
                   
               
               
                   
                 GAAGAACAGCAGGUGUCCCUAGAAUCCGUGGAUGUGUAC 
                   
               
               
                   
                 UUCCAGGACGUUUUCGGCACAAUGUGGUGCCAUCACGCA 
                   
               
               
                   
                 GAGAUGCAAAAUCCGGUGUACCUUAUCCCCGAAACAGUG 
                   
               
               
                   
                 CCGUACAUUAAAUGGGAUAAUUGUAACUCCACAAACAUC 
                   
               
               
                   
                 ACGGCAGUGGUGCGCGCUCAGGGGCUGGACGUGACACUC 
                   
               
               
                   
                 CCCCUUUCCUUACCAACAAGUGCUCAAGAUUCCAACUUU 
                   
               
               
                   
                 UCUGUGAAAACCGAGAUGCUGGGCAACGAGAUUGAUAUU 
                   
               
               
                   
                 GAAUGCAUCAUGGAGGAUGGCGAGAUUAGCCAGGUGUUG 
                   
               
               
                   
                 CCCGGCGACAAUAAAUUUAAUAUUACCUGUUCCGGCUAU 
                   
               
               
                   
                 GAGUCACACGUGCCAAGUGGAGGCAUUCUCACGAGCACC 
                   
               
               
                   
                 UCUCCCGUUGCAACACCCAUUCCUGGGACAGGGUACGCA 
                   
               
               
                   
                 UAUAGUCUCAGGCUGACACCGAGACCGGUAUCGCGCUUC 
                   
               
               
                   
                 UUAGGUAACAAUUCCAUCUUGUAUGUGUUUUACAGCGGA 
                   
               
               
                   
                 AACGGGCCAAAGGCCUCAGGAGGAGACUACUGCAUCCAG 
                   
               
               
                   
                 AGCAAUAUAGUUUUUAGCGACGAGAUCCCUGCCUCACAA 
                   
               
               
                   
                 GACAUGCCCACCAACACCACCGACAUCACGUACGUCGGUG 
                   
               
               
                   
                 AUAAUGCCACUUACAGCGUGCCUAUGGUGACCUCUGAAG 
                   
               
               
                   
                 ACGCAAAUUCACCCAAUGUCACGGUUACCGCAUUCUGGG 
                   
               
               
                   
                 CUUGGCCAAAUAACACUGAAACCGACUUCAAGUGUAAAU 
                   
               
               
                   
                 GGACCCUAACCAGCGGCACCCCAUCAGGGUGCGAGAAUA 
                   
               
               
                   
                 UCAGCGGGGCCUUCGCCAGCAACCGGACAUUCGACAUCAC 
                   
               
               
                   
                 CGUGUCAGGACUAGGGACUGCUCCAAAAACAUUAAUUAU 
                   
               
               
                   
                 UACACGGACAGCAACUAACGCUACGACUACGACUCACAA 
                   
               
               
                   
                 AGUGAUAUUUAGCAAAGCCCCCGAAUCGACUACUACGAG 
                   
               
               
                   
                 CCCGACCCUAAACACCACCGGUUUCGCCGAUCCCAACACA 
                   
               
               
                   
                 ACUACUGGUCUCCCAUCUUCGACCCAUGUUCCAACGAAUC 
                   
               
               
                   
                 UGACAGCUCCAGCAAGUACGGGGCCCACUGUGAGUACAG 
                   
               
               
                   
                 CCGACGUUACUAGCCCAACUCCCGCGGGUACGACUAGUG 
                   
               
               
                   
                 GCGCGUCUCCAGUGACCCCUUCGCCUUCUCCGUGGGAUAA 
                   
               
               
                   
                 UGGAACCGAGUCAAAGGCUCCUGAUAUGACCAGCUCCAC 
                   
               
               
                   
                 CAGUCCCGUUACAACGCCAACCCCCAAUGCAACGAGUCCA 
                   
               
               
                   
                 ACUCCGGCGGUUACCACUCCGACACCAAACGCCACCUCCC 
                   
               
               
                   
                 CCACGCCUGCAGUUACUACGCCCACCCCUAAUGCUACGUC 
                   
               
               
                   
                 ACCCACUCUGGGAAAGACCUCACCAACCUCUGCCGUGACU 
                   
               
               
                   
                 ACUCCUACACCUAAUGCCACAGGACCCACAGUUGGGGAA 
                   
               
               
                   
                 ACCAGCCCACAGGCUAACGCAACUAAUCAUACGCUUGGG 
                   
               
               
                   
                 GGGACCUCUCCCACGCCCGUGGUCACUUCACAGCCUAAGA 
                   
               
               
                   
                 AUGCCACAAGCGCUGUGACUACAGGCCAGCAUAAUAUAA 
                   
               
               
                   
                 CCAGCAGUUCAACCAGUAGCAUGUCCCUGCGUCCUUCAUC 
                   
               
               
                   
                 CAAUCCUGAGACUCUGAGCCCCAGCACUAGUGACAACUC 
                   
               
               
                   
                 AACUUCUCACAUGCCGUUGCUGACCUCCGCGCAUCCCACG 
                   
               
               
                   
                 GGCGGUGAGAAUAUCACACAGGUGACACCCGCGUCUAUU 
                   
               
               
                   
                 AGCACACAUCACGUGAGUACGUCCUCCCCCGCACCCCGCC 
                   
               
               
                   
                 CAGGCACAACCUCACAAGCAUCAGGUCCGGGGAACAGCA 
                   
               
               
                   
                 GCACAAGUACCAAGCCUGGCGAAGUGAAUGUAACCAAGG 
                   
               
               
                   
                 GCACGCCCCCGCAGAACGCCACAUCCCCUCAAGCCCCAUC 
                   
               
               
                   
                 CGGCCAGAAGACCGCUGUACCCACCGUGACAUCAACUGG 
                   
               
               
                   
                 GGGGAAAGCGAACUCAACUACCGGGGGGAAGCAUACAAC 
                   
               
               
                   
                 AGGGCACGGUGCCAGAACUUCUACAGAGCCAACCACGGA 
                   
               
               
                   
                 CUACGGAGGGGACAGCACAACGCCCAGACCGCGGUACAA 
                   
               
               
                   
                 UGCGACCACAUACCUGCCUCCCUCAACUUCGAGUAAGCUG 
                   
               
               
                   
                 CGACCCAGAUGGACCUUUACUUCUCCCCCUGUCACCACCG 
                   
               
               
                   
                 CUCAAGCCACCGUCCCGGUUCCACCUACUUCCCAGCCUAG 
                   
               
               
                   
                 AUUCUCCAAUCUGAGCAUGCUUGUCCUACAGUGGGCAUC 
                   
               
               
                   
                 ACUCGCGGUACUAACCUUGCUGCUACUGUUGGUGAUGGC 
                   
               
               
                   
                 GGACUGCGCGUUUCGCCGCAACUUAUCCACCUCCCACACG 
                   
               
               
                   
                 UACACAACACCUCCCUAUGAUGACGCUGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  28 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp42 (B95-8)_DX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 155 consists of from 5′ end to 3′ end: 
                 155 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 33, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGUGAGCUUCAAGCAGGUGAGAGUGCCCCUGUUCACC 
                  33 
               
               
                 Construct 
                 GCCAUCGCCCUGGUGAUCGUGCUGCUGCUGGCCUACUUCC 
                   
               
               
                 (excluding 
                 UGCCCCCCAGAGUGAGAGGCGGCGGCAGAGUGGCCGCCG 
                   
               
               
                 the stop 
                 CCGCCAUCACCUGGGUGCCCAAGCCCAACGUGGAGGUGU 
                   
               
               
                 codon) 
                 GGCCCGUGGACCCCCCACCCCCCGUGAACUUCAACAAGAC 
                   
               
               
                   
                 CGCCGAGCAGGAGUACGGCGACAAGGAGGUGAAGCUGCC 
                   
               
               
                   
                 CCACUGGACCCCCACCCUGCACACCUUCCAGGUGCCCCAG 
                   
               
               
                   
                 AACUACACCAAGGCCAACUGCACCUACUGCAACACCAGAG 
                   
               
               
                   
                 AGUACACCUUCAGCUACAAGGGCUGCUGCUUCUACUUCA 
                   
               
               
                   
                 CCAAGAAGAAGCACACCUGGAACGGCUGCUUCCAGGCCU 
                   
               
               
                   
                 GCGCCGAGCUGUACCCCUGCACCUACUUCUACGGCCCCAC 
                   
               
               
                   
                 CCCCGACAUCCUGCCCGUGGUGACCAGAAACCUGAACGCC 
                   
               
               
                   
                 AUCGAGAGCCUGUGGGUGGGCGUGUACAGAGUGGGCGAG 
                   
               
               
                   
                 GGCAACUGGACCAGCCUGGACGGCGGCACCUUCAAGGUG 
                   
               
               
                   
                 UACCAGAUCUUCGGCAGCCACUGCACCUACGUGAGCAAG 
                   
               
               
                   
                 UUCAGCACCGUGCCCGUGAGCCACCACGAGUGCAGCUUCC 
                   
               
               
                   
                 UGAAGCCCUGCCUGUGCGUGAGCCAGAGAAGCAACAGC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MVSFKQVRVPLFTAIALVIVLLLAYFLPPRVRGGGRVAAAAIT 
                  34 
               
               
                 amino acid 
                 WVPKPNVEVWPVDPPPPVNFNKTAEQEYGDKEVKLPHWTPT 
                   
               
               
                 sequence 
                 LHTFQVPQNYTKANCTYCNTREYTFSYKGCCFYFTKKKHTW 
                   
               
               
                   
                 NGCFQACAELYPCTYFYGPTPDILPVVTRNLNAIESLWVGVYR 
                   
               
               
                   
                 VGEGNWTSLDGGTFKVYQIFGSHCTYVSKFSTVPVSHHECSFL 
                   
               
               
                   
                 KPCLCVSQRSNS 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gL (B95-8) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 156 consists of from 5′ end to 3′ end: 
                 156 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 35, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGAGCCGUGGGCGUGUUCCUGGCCAUCUGCCUGGUG 
                  35 
               
               
                 Construct 
                 ACCAUCUUCGUGCUGCCCACCUGGGGCAACUGGGCCUACC 
                   
               
               
                 (excluding 
                 CCUGCUGCCACGUGACCCAGCUGAGAGCCCAGCACCUGCU 
                   
               
               
                 the stop 
                 GGCCCUGGAGAACAUCAGCGACAUCUACCUGGUGAGCAA 
                   
               
               
                 codon) 
                 CCAGACCUGCGACGGCUUCAGCCUGGCCAGCCUGAACAGC 
                   
               
               
                   
                 CCCAAGAACGGCAGCAACCAGCUGGUGAUCAGCAGAUGC 
                   
               
               
                   
                 GCCAACGGCCUGAACGUGGUGAGCUUCUUCAUCAGCAUC 
                   
               
               
                   
                 CUGAAGAGAAGCAGCAGCGCCCUGACCGGCCACCUGAGA 
                   
               
               
                   
                 GAGCUGCUGACCACCCUGGAGACCCUGUACGGCAGCUUC 
                   
               
               
                   
                 AGCGUGGAGGACCUGUUCGGCGCCAACCUGAACAGAUAC 
                   
               
               
                   
                 GCCUGGCACAGAGGCGGC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MRAVGVFLAICLVTIFVLPTWGNWAYPCCHVTQLRAQHLLA 
                  36 
               
               
                 amino acid 
                 LENISDIYLVSNQTCDGFSLASLNSPKNGSNQLVISRCANGLNV 
                   
               
               
                 sequence 
                 VSFFISILKRSSSALTGHLRELLTTLETLYGSFSVEDLFGANLNR 
                   
               
               
                   
                 YAWHRGG 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gH (BXLF2)_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 157 consists of from 5′ end to 3′ end: 
                 157 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 37, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGCAGUUGUUGUGCGUGUUCUGCCUCGUGUUACUCUGG 
                  37 
               
               
                 Construct 
                 GAGGUGGGCGCCGCCAGCCUUAGCGAGGUGAAGCUCCAC 
                   
               
               
                 (excluding 
                 UUGGACAUCGAGGGCCACGCCAGCCACUACACCAUCCCCU 
                   
               
               
                 the stop 
                 GGACCGAGCUCAUGGCCAAGGUGCCCGGCCUUAGCCCCGA 
                   
               
               
                 codon) 
                 GGCCCUGUGGCGGGAGGCCAACGUGACCGAGGACCUGGC 
                   
               
               
                   
                 CAGCAUGCUGAACCGGUACAAGCUGAUCUACAAGACCAG 
                   
               
               
                   
                 CGGCACCCUGGGCAUCGCCCUGGCCGAGCCCGUGGACAUC 
                   
               
               
                   
                 CCCGCCGUUAGCGAAGGCAGCAUGCAGGUGGACGCCAGC 
                   
               
               
                   
                 AAGGUGCACCCCGGCGUGAUCAGCGGCCUGAACAGCCCCG 
                   
               
               
                   
                 CCUGUAUGUUGAGCGCCCCACUGGAGAAGCAGCUGUUCU 
                   
               
               
                   
                 ACUACAUCGGCACCAUGCUGCCCAACACCCGGCCCCACAG 
                   
               
               
                   
                 CUACGUGUUCUACCAGCUGCGGUGCCACCUGAGCUACGU 
                   
               
               
                   
                 UGCCCUGAGCAUCAACGGCGACAAGUUCCAGUACACCGG 
                   
               
               
                   
                 CGCCAUGACCAGCAAGUUCCUGAUGGGCACCUACAAGCG 
                   
               
               
                   
                 GGUCACCGAGAAGGGCGACGAGCACGUGCUGUCACUGGU 
                   
               
               
                   
                 GUUCGGCAAGACCAAGGACCUGCCCGACCUGCGGGGCCCC 
                   
               
               
                   
                 UUCAGCUACCCUAGUUUGACCAGCGCCCAGAGCGGCGAC 
                   
               
               
                   
                 UACAGCUUGGUGAUCGUGACCACCUUCGUGCACUACGCC 
                   
               
               
                   
                 AACUUCCACAACUACUUCGUGCCCAACCUGAAGGACAUG 
                   
               
               
                   
                 UUCAGCCGGGCCGUGACCAUGACUGCCGCUUCUUACGCCC 
                   
               
               
                   
                 GGUACGUGCUGCAGAAGCUGGUCCUGCUGGAGAUGAAGG 
                   
               
               
                   
                 GCGGCUGCCGGGAGCCCGAGCUGGACACCGAAACACUGA 
                   
               
               
                   
                 CCACCAUGUUCGAGGUGAGCGUGGCCUUCUUCAAGGUGG 
                   
               
               
                   
                 GUCACGCGGUGGGCGAAACCGGCAACGGCUGCGUGGACU 
                   
               
               
                   
                 UACGCUGGCUGGCCAAGAGCUUCUUCGAGCUGACCGUGC 
                   
               
               
                   
                 UGAAGGAUAUCAUCGGCAUCUGCUACGGCGCCACCGUGA 
                   
               
               
                   
                 AGGGCAUGCAGAGCUACGGCCUGGAGCGGCUGGCCGCCA 
                   
               
               
                   
                 UGCUUAUGGCAACAGUGAAGAUGGAGGAGCUGGGACACC 
                   
               
               
                   
                 UGACAACAGAGAAGCAGGAGUACGCCCUGAGACUGGCCA 
                   
               
               
                   
                 CAGUGGGCUACCCAAAGGCCGGCGUGUACAGUGGACUGA 
                   
               
               
                   
                 UCGGCGGCGCAACCAGCGUGCUGCUAUCCGCUUACAACCG 
                   
               
               
                   
                 GCACCCGCUGUUCCAGCCCCUGCACACCGUGAUGCGGGAA 
                   
               
               
                   
                 ACCCUGUUCAUCGGAAGCCACGUCGUGCUGCGGGAGCUG 
                   
               
               
                   
                 AGGCUGAACGUAACCACCCAGGGCCCUAAUCUGGCCCUG 
                   
               
               
                   
                 UAUCAGCUCCUCAGUACCGCCCUGUGCAGCGCCCUUGAGA 
                   
               
               
                   
                 UCGGCGAGGUGCUCAGAGGCCUGGCCCUCGGUACCGAGA 
                   
               
               
                   
                 GCGGCCUCUUCAGCCCAUGCUACUUAAGCCUGCGGUUCG 
                   
               
               
                   
                 ACCUGACCCGGGACAAGUUGCUGAGCAUGGCCCCGCAGG 
                   
               
               
                   
                 AGGCCACACUGGACCAGGCAGCUGUAUCCAACGCCGUGG 
                   
               
               
                   
                 ACGGCUUCCUGGGCAGACUGUCCCUGGAACGGGAGGACC 
                   
               
               
                   
                 GGGACGCCUGGCACCUGCCUGCCUACAAGUGUGUGGAUC 
                   
               
               
                   
                 GGCUGGACAAGGUGCUGAUGAUCAUCCCUCUGAUUAAUG 
                   
               
               
                   
                 UCACCUUCAUCAUCAGCAGCGACCGGGAGGUGCGGGGAU 
                   
               
               
                   
                 CCGCCCUCUACGAGGCCAGCACCACCUAUCUGAGCAGCAG 
                   
               
               
                   
                 CCUGUUCCUGUCUCCUGUGAUCAUGAACAAGUGCAGCCA 
                   
               
               
                   
                 GGGCGCCGUGGCCGGCGAGCCCCGGCAGAUCCCCAAGAUC 
                   
               
               
                   
                 CAGAACUUCACCCGGACCCAGAAGUCUUGCAUCUUCUGC 
                   
               
               
                   
                 GGCUUCGCCCUUUUGUCCUACGACGAGAAGGAGGGCUUG 
                   
               
               
                   
                 GAGACUACAACCUACAUCACCAGCCAGGAGGUGCAGAAC 
                   
               
               
                   
                 AGCAUCCUGUCAUCUAAUUACUUCGACUUCGACAACCUG 
                   
               
               
                   
                 CACGUUCAUUACCUGCUCCUCACCACCAACGGUACCGUCA 
                   
               
               
                   
                 UGGAAAUCGCCGGACUGUACGAGGAGCGGGCCCAUGUUG 
                   
               
               
                   
                 UGCUGGCCAUCAUCCUGUACUUCAUCGCUUUCGCACUUG 
                   
               
               
                   
                 GCAUCUUCCUGGUGCACAAGAUCGUGAUGUUCUUCCUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MQLLCVFCLVLLWEVGAASLSEVKLHLDIEGHASHYTIPWTE 
                  38 
               
               
                 amino acid 
                 LMAKVPGLSPEALWREANVTEDLASMLNRYKLIYKTSGTLGI 
                   
               
               
                 sequence 
                 ALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSPACMLSAPLE 
                   
               
               
                   
                 KQLFYYIGTMLPNTRPHSYVFYQLRCHLSYVALSINGDKFQYT 
                   
               
               
                   
                 GAMTSKFLMGTYKRVTEKGDEHVLSLVFGKTKDLPDLRGPFS 
                   
               
               
                   
                 YPSLTSAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMFSRAV 
                   
               
               
                   
                 TMTAASYARYVLQKLVLLEMKGGCREPELDTETLTTMFEVS 
                   
               
               
                   
                 VAFFKVGHAVGETGNGCVDLRWLAKSFFELTVLKDIIGICYG 
                   
               
               
                   
                 ATVKGMQSYGLERLAAMLMATVKMEELGHLTTEKQEYALR 
                   
               
               
                   
                 LATVGYPKAGVYSGLIGGATSVLLSAYNRHPLFQPLHTVMRE 
                   
               
               
                   
                 TLFIGSHVVLRELRLNVTTQGPNLALYQLLSTALCSALEIGEVL 
                   
               
               
                   
                 RGLALGTESGLFSPCYLSLRFDLTRDKLLSMAPQEATLDQAA 
                   
               
               
                   
                 VSNAVDGFLGRLSLEREDRDAWHLPAYKCVDRLDKVLMIIPL 
                   
               
               
                   
                 INVTFIISSDREVRGSALYEASTTYLSSSLFLSPVIMNKCSQGAV 
                   
               
               
                   
                 AGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKEGLETTTYITSQ 
                   
               
               
                   
                 EVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEIAGLYEERAH 
                   
               
               
                   
                 VVLAIILYFIAFALGIFLVHKIVMFFL 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV LMP1_DX (modified) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 158 consists of from 5′ end to 3′ end: 
                 158 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 39, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAACACGACCUUGAGAGGGGCCCACCGGGCCCGCGA 
                  39 
               
               
                 Construct 
                 CGGCCACCUCGAGGACCUCCCCUCUCCUCUUCCCUAGGCC 
                   
               
               
                 (excluding 
                 UUGCUCUCCUUCUCCUCCUCUUGGCGCUACUGUUUUGGC 
                   
               
               
                 the stop 
                 UGUACAUCGUUAUGAGUGACUGGACUGGAGGAGCCCUCC 
                   
               
               
                 codon) 
                 UUGUCCUCUAUUCCUUUGCUCUCAUGCUUAUAAUUAUAA 
                   
               
               
                   
                 UUUUGAUCAUCUUUAUCUUCAGAAGAGACCUUCUCUGUC 
                   
               
               
                   
                 CACUUGGAGCCCUUUGUAUACUCCUACUGAUGAUCACCC 
                   
               
               
                   
                 UCCUGCUCAUCGCUCUCUGGAAUUUGCACGGACAGGCAU 
                   
               
               
                   
                 UGUUCCUUGGAAUUGUGCUGUUCAUCUUCGGGUGCUUAC 
                   
               
               
                   
                 UUGUCUUAGGUAUCUGGAUCUACUUAUUGGAGAUGCUCU 
                   
               
               
                   
                 GGCGACUUGGUGCCACCAUCUGGCAGCUUUUGGCCUUCU 
                   
               
               
                   
                 UCCUAGCCUUCUUCCUAGACCUCAUCCUGCUCAUUAUUGC 
                   
               
               
                   
                 UCUCUAUCUACAACAGAACUGGUGGACUCUAUUGGUUGA 
                   
               
               
                   
                 UCUCCUUUGGCUCCUCCUGUUUCUGGCGAUUUUAAUCUG 
                   
               
               
                   
                 GAUGUAUUACCAUGGACAACGACACAGUGAUGAACACCA 
                   
               
               
                   
                 CCACGAUGACUCCCUCCCGCACCCUCAACAAGCUACCGAU 
                   
               
               
                   
                 GAUUCUGGCCAUGAAUCUGACUCUAACUCCAACGAGGGC 
                   
               
               
                   
                 AGACACCACCUGCUCGUGAGUGGAGCCGGCGACGGACCA 
                   
               
               
                   
                 CCACUCUGCUCUCAGAACCUAGGCGCACCUGGAGGUGGU 
                   
               
               
                   
                 CCUGACAAUGGCCCACAGGACCCUGACAACACGGAUGAU 
                   
               
               
                   
                 AAUGGCCCACAGGACCCUGAUAACACUGAUGACAAUGGC 
                   
               
               
                   
                 CCACAUGACCCGCUGCCUCAAGACCCAGACAAUACUGAUG 
                   
               
               
                   
                 ACAAUGGCCCACAGGACCCUGACAACACUGAUGACAAUG 
                   
               
               
                   
                 GCCCGCACGACCCGUUACCUCAUAGCCCUAGCGACUCUGC 
                   
               
               
                   
                 UGGAAAUGAUGGAGGCCCUCCACAAUUGACGGAAGAGGU 
                   
               
               
                   
                 UGAGAACAAAGGAGGUGACCAGGGCCCGCCUUUGAUGAC 
                   
               
               
                   
                 AGACGGAGGCGGCGGUCAUAGUCAUGAUUCCGGCCAUGG 
                   
               
               
                   
                 CGGCGGUGAUCCACACCUUCCUACGCUGCUGUUGGGUUC 
                   
               
               
                   
                 UUCUGGUUCCGGUGGAGAUGAUGACGACCCGCACGGCCC 
                   
               
               
                   
                 AGUUCAGCUAAGCUACUAUGAC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEHDLERGPPGPRRPPRGPPLSSSLGLALLLLLLALLFWLYIV 
                  16 
               
               
                 amino acid 
                 MSDWTGGALLVLYSFALMLIIIILIIFIFRRDLLCPLGALCILLLM 
                   
               
               
                 sequence 
                 ITLLLIALWNLHGQALFLGIVLFIFGCLLVLGIWIYLLEMLWRL 
                   
               
               
                   
                 GATIWQLLAFFLAFFLDLILLIIALYLQQNWWTLLVDLLWLLL 
                   
               
               
                   
                 FLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSN 
                   
               
               
                   
                 SNEGRHHLLVSGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTD 
                   
               
               
                   
                 DNGPQDPDNTDDNGPHDPLPQDPDNTDDNGPQDPDNTDDNG 
                   
               
               
                   
                 PHDPLPHSPSDSAGNDGGPPQLTEEVENKGGDQGPPLMTDGG 
                   
               
               
                   
                 GGHSHDSGHGGGDPHLPTLLLGSSGSGGDDDDPHGPVQLSYY 
                   
               
               
                   
                 D 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV LMP2 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 159 consists of from 5′ end to 3′ end: 
                 159 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 40, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGGUCCCUAGAAAUGGUGCCAAUGGGCGCGGGUCCU 
                  40 
               
               
                 Construct 
                 CCUAGCCCCGGCGGGGAUCCGGAUGGGUACGAUGGCGGA 
                   
               
               
                 (excluding 
                 AACAACUCCCAAUAUCCAUCUGCUUCUGGCUCUUCUGGG 
                   
               
               
                 the stop 
                 AACACGCCCACCCCACCGAACGAUGAGGAACGUGAAUCU 
                   
               
               
                 codon) 
                 AAUGAAGAGCCACCACCGCCUUAUGAGGACCCAUAUUGG 
                   
               
               
                   
                 GGCAAUGGCGACCGUCACUCGGACUAUCAACCACUAGGA 
                   
               
               
                   
                 ACCCAAGAUCAAAGUCUGUACUUGGGAUUGCAACACGAC 
                   
               
               
                   
                 GGGAAUGACGGGCUCCCUCCACCUCCCUACUCUCCACGGG 
                   
               
               
                   
                 AUGACUCAUCUCAACACAUAUACGAAGAAGCGGGCAGAG 
                   
               
               
                   
                 GAAGUAUGAAUCCAGUAUGCCUGCCUGUAAUUGUUGCGC 
                   
               
               
                   
                 CCUACCUCUUUUGGCUGGCGGCUAUUGCCGCCUCGUGUU 
                   
               
               
                   
                 UCACGGCCUCAGUUAGUACCGUUGUGACCGCCACCGGCU 
                   
               
               
                   
                 UGGCCCUCUCACUUCUACUCUUGGCAGCAGUGGCCAGCUC 
                   
               
               
                   
                 AUAUGCCGCUGCACAAAGGAAACUGCUGACACCGGUGAC 
                   
               
               
                   
                 AGUGCUUACUGCGGUUGUCACUUUCUUUGCAAUUUGCCU 
                   
               
               
                   
                 AACAUGGAGGAUUGAGGACCCACCUUUUAAUUCUCUUCU 
                   
               
               
                   
                 GUUUGCAUUGCUGGCCGCAGCUGGCGGACUACAAGGCAU 
                   
               
               
                   
                 UUACGUUCUGGUGAUGCUUGUGCUCCUGAUACUAGCGUA 
                   
               
               
                   
                 CAGAAGGAGAUGGCGCCGUUUGACUGUUUGUGGCGGCAU 
                   
               
               
                   
                 CAUGUUCUUGGCAUGUGUACUUGUCCUCAUCGUCGACGC 
                   
               
               
                   
                 UGUUUUGCAGCUGAGUCCCCUCCUUGGAGCUGUAACUGU 
                   
               
               
                   
                 GGUUUCCAUGACGCUGCUGCUACUGGCUUUCGUCCUCUG 
                   
               
               
                   
                 GCUCUCUUCGCCAGGUGGCCUAGGUACUCUUGGUGCAGC 
                   
               
               
                   
                 CCUUUUAACAUUGGCAGCAGCUCUGGCACUGCUAGCGUC 
                   
               
               
                   
                 ACUGAUUUUGGGCACACUUAACUUGACUACAAUGUUCCU 
                   
               
               
                   
                 UCUCAUGCUCCUAUGGACACUUGUGGUUCUCCUGAUUUG 
                   
               
               
                   
                 CUCUUCGUGCUCUUCAUGUCCACUGAGCAAGAUCCUUCU 
                   
               
               
                   
                 GGCACGACUGUUCCUAUAUGCUCUCGCACUCUUGUUGCU 
                   
               
               
                   
                 AGCCUCCGCGCUAAUCGCUGGUGGCAGUAUUUUGCAAAC 
                   
               
               
                   
                 AAACUUCAAGAGUUUAAGCAGCACUGAAUUUAUACCCAA 
                   
               
               
                   
                 UUUGUUCUGCAUGUUAUUACUGAUUGUCGCUGGCAUACU 
                   
               
               
                   
                 CUUCAUUCUUGCUAUCCUGACCGAAUGGGGCAGUGGAAA 
                   
               
               
                   
                 UAGAACAUACGGUCCAGUCUUUAUGUGCCUCGGUGGCCU 
                   
               
               
                   
                 GCUCACCAUGGUAGCCGGCGCUGUGUGGCUGACGGUGAU 
                   
               
               
                   
                 GUCUAACACGCUUUUGUCUGCCUGGAUUCUUACAGCAGG 
                   
               
               
                   
                 AUUCCUGAUUUUCCUCAUUGGCUUUGCCCUCUUUGGGGU 
                   
               
               
                   
                 CAUUAGAUGCUGCCGCUACUGCUGCUACUACUGCCUUAC 
                   
               
               
                   
                 ACUGGAAAGUGAGGAGCGCCCACCGACCCCAUAUCGCAA 
                   
               
               
                   
                 CACUGUA 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNT 
                  22 
               
               
                 amino acid 
                 PTPPNDEERESNEEPPPPYEDPYWGNGDRHSDYQPLGTQDQSL 
                   
               
               
                 sequence 
                 YLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLP 
                   
               
               
                   
                 VIVAPYLFWLAAIAASCFTASVSTVVTATGLALSLLLLAAVAS 
                   
               
               
                   
                 SYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLFALL 
                   
               
               
                   
                 AAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVL 
                   
               
               
                   
                 VLIVDAVLQLSPLLGAVTVVSMTLLLLAFVLWLSSPGGLGTL 
                   
               
               
                   
                 GAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICS 
                   
               
               
                   
                 SCSSCPLSKILLARLFLYALALLLLASALIAGGSILQTNFKSLSS 
                   
               
               
                   
                 TEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTYGPVFMCLGG 
                   
               
               
                   
                 LLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGVIRC 
                   
               
               
                   
                 CRYCCYYCLTLESEERPPTPYRNTV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_trunc_001 (modified) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 160 consists of from 5′ end to 3′ end: 
                 160 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 41, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGCAGAAGACCCUUCUUUCACCCCGUGGGAGAAGCC 
                  41 
               
               
                 Construct 
                 GACUAUUUCGAGUACCACCAGGAGGGCGGUCCUGACGGC 
                   
               
               
                 (excluding 
                 GAGCCCGACGUGCCUCCAGGCGCCAUCGAACAGGGCCCCG 
                   
               
               
                 the stop 
                 CUGACGAUCCUGGCGAGGGGCCUUCCACAGGCCCUCGCGG 
                   
               
               
                 codon) 
                 CCAGGGAGAUGGCGGAAGGCGGAAGAAGGGCGGCUGGUU 
                   
               
               
                   
                 UGGCAAGCAUAGGGGCCAGGGCGGCUCCAAUCCCAAGUU 
                   
               
               
                   
                 CGAGAAUAUCGCCGAGGGGCUCCGGGCUCUGCUGGCCCG 
                   
               
               
                   
                 GUCGCACGUCGAACGUACCACUGACGAGGGGACGUGGGU 
                   
               
               
                   
                 GGCCGGCGUCUUUGUGUACGGAGGAUCCAAGACCUCCCU 
                   
               
               
                   
                 GUACAACCUGAGAAGGGGCACCGCCCUUGCCAUCCCUCAG 
                   
               
               
                   
                 UGCAGACUCACGCCCCUGAGCAGGCUCCCCUUUGGGAUG 
                   
               
               
                   
                 GCUCCUGGGCCAGGCCCGCAGCCCGGGCCCCUCAGGGAAA 
                   
               
               
                   
                 GCAUCGUGUGUUACUUUAUGGUGUUUCUGCAGACACACA 
                   
               
               
                   
                 UCUUUGCAGAAGUUCUGAAAGAUGCAAUCAAGGAUCUGG 
                   
               
               
                   
                 UGAUGACCAAGCCUGCACCUACGUGCAAUAUCAGGGUGA 
                   
               
               
                   
                 CAGUAUGCUCCUUCGACGACGGUGUGGACCUCCCGCCCUG 
                   
               
               
                   
                 GUUCCCACCCAUGGUCGAGGGUGCCGCCGCCGAGGGAGA 
                   
               
               
                   
                 CGAUGGCGACGACGGUGAUGAGGGUGGAGACGGAGAUGA 
                   
               
               
                   
                 AGGCGAGGAGGGGCAGGAG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADD 
                  10 
               
               
                 amino acid 
                 PGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIA 
                   
               
               
                 sequence 
                 EGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRG 
                   
               
               
                   
                 TALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQ 
                   
               
               
                   
                 THIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWF 
                   
               
               
                   
                 PPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV EBNA3 (B95-8)_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 161 consists of from 5′ end to 3′ end: 
                 161 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 42, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGACAAGGACAGGCCCGGCCCUCCCGCCUUGGACGACA 
                  42 
               
               
                 Construct 
                 ACAUGGAGGAGGAGGUGCCCAGCACCAGCGUGGUGCAGG 
                   
               
               
                 (excluding 
                 AGCAGGUGAGCGCCGGCGACUGGGAGAACGUGUUAAUCG 
                   
               
               
                 the stop 
                 AGUUGAGCGACAGCAGCAGCGAGAAGGAGGCCGAGGACG 
                   
               
               
                 codon) 
                 CCCACCUGGAGCCCGCCCAGAAGGGCACCAAGAGGAAGA 
                   
               
               
                   
                 GGGUGGACCACGACGCUGGCGGAUCUGCCCCUGCCAGGCC 
                   
               
               
                   
                 CAUGCUGCCACCCCAGCCCGACCUGCCCGGCAGGGAGGCC 
                   
               
               
                   
                 AUCCUGAGGAGGUUCCCACUGGACCUGAGGACCCUGCUG 
                   
               
               
                   
                 CAGGCCAUCGGCGCCGCCGCCACCAGGAUCGACACCAGGG 
                   
               
               
                   
                 CCAUCGACCAGUUCUUCGGCAGCCAGAUCAGCAACACCGA 
                   
               
               
                   
                 GAUGUACAUCAUGUACGCCAUGGCCAUCAGACAAGCAAU 
                   
               
               
                   
                 UAGGGAUCGACGCAGAAAUCCUGCCAGCAGGAGGGACCA 
                   
               
               
                   
                 GGCCAAGUGGAGGCUGCAGACCCUGGCCGCCGGCUGGCCC 
                   
               
               
                   
                 AUGGGCUACCAGGCCUACAGCAGCUGGAUGUACAGCUAC 
                   
               
               
                   
                 ACCGACCACCAGACCACUCCGACCUUCGUGCACCUCCAGG 
                   
               
               
                   
                 CUACCCUGGGCUGCACUGGCGGUCGCAGGUGCCACGUGA 
                   
               
               
                   
                 CCUUCUCAGCGGGCACCUUCAAGCUGCCCAGGUGCACACC 
                   
               
               
                   
                 UGGCGACAGGCAGUGGCUGUACGUGCAGAGCAGCGUGGG 
                   
               
               
                   
                 CAACAUUGUUCAGAGUUGCAACCCCAGGUACAGCAUCUU 
                   
               
               
                   
                 CUUCGACUACAUGGCGAUUCACAGGAGCCUGACCAAGAU 
                   
               
               
                   
                 CUGGGAGGAAGUCCUGACCCCUGACCAGAGGGUGAGCUU 
                   
               
               
                   
                 CAUGGAGUUCCUGGGCUUCCUGCAGAGGACCGACCUGAG 
                   
               
               
                   
                 CUACAUCAAGAGCUUCGUGAGCGACGCCCUGGGCACCACC 
                   
               
               
                   
                 AGCAUCCAGACUCCUUGGAUCGACGAUAACCCUAGCACC 
                   
               
               
                   
                 GAAACCGCCCAGGCCUGGAACGCCGGUUUCCUGAGGGGC 
                   
               
               
                   
                 AGGGCCUACGGCAUCGACCUGCUCCGGACCGAGGGCGAG 
                   
               
               
                   
                 CACGUGGAGGGCGCCACCGGCGAGACUAGGGAGGAGAGC 
                   
               
               
                   
                 GAGGACACUGAGAGCGACGGCGACGACGAGGAUCUGCCG 
                   
               
               
                   
                 UGCAUCGUGAGCAGGGGCGGCCCCAAGGUGAAGAGGCCU 
                   
               
               
                   
                 CCCAUCUUCAUCCGGAGGCUUCACAGGCUGCUGCUGAUG 
                   
               
               
                   
                 AGGGCCGGCAAGCGUACUGAACAGGGCAAGGAGGUACUG 
                   
               
               
                   
                 GAGAAGGCCAGGGGCAGCACAUAUGGCACCCCUAGACCG 
                   
               
               
                   
                 CCUGUGCCCAAGCCGCGUCCUGAAGUCCCGCAGAGUGAU 
                   
               
               
                   
                 GAGACAGCGACUAGCCAUGGCAGUGCUCAGGUGCCCGAG 
                   
               
               
                   
                 CCACCCACCAUCCAUCUCGCCGCACAGGGCAUGGCCUACC 
                   
               
               
                   
                 CACUGCACGAGCAGCACGGCAUGGCGCCCUGCCCCGUGGC 
                   
               
               
                   
                 CCAGGCUCCUCCAACCCCGCUUCCUCCAGUAUCCCCUGGU 
                   
               
               
                   
                 GACCAGUUGCCAGGCGUGUUCAGCGACGGUAGGGUGGCC 
                   
               
               
                   
                 UGCGCGCCAGUUCCUGCACCAGCAGGCCCAAUUGUGAGG 
                   
               
               
                   
                 CCCUGGGAGCCCUCCCUAACCCAGGCGGCAGGUCAGGCCU 
                   
               
               
                   
                 UCGCUCCAGUUAGACCACAGCACAUGCCCGUGGAACCGG 
                   
               
               
                   
                 UGCCAGUGCCUACGGUGGCGCUGGAGAGGCCCGUGUACC 
                   
               
               
                   
                 CAAAGCCUGUGCGACCUGCGCCUCCUAAGAUCGCCAUGCA 
                   
               
               
                   
                 AGGCCCGGGCGAGACUAGCGGUAUCAGGCGGGCCAGGGA 
                   
               
               
                   
                 GAGGUGGCGCCCGGCACCGUGGACUCCCAACCCACCAAGG 
                   
               
               
                   
                 UCACCUAGUCAGAUGAGCGUGCGUGAUAGGCUGGCACGG 
                   
               
               
                   
                 CUUCGGGCAGAGGCUCAGGUAAAGCAGGCCAGUGUGGAG 
                   
               
               
                   
                 GUGCAGCCACCACAGCUGACUCAGGUGUCCCCGCAGCAGC 
                   
               
               
                   
                 CCAUGGAGGGCCCACUGGUGCCGGAACAGCAGAUGUUCC 
                   
               
               
                   
                 CCGGCGCCCCGUUCAGCCAGGUGGCCGACGUGGUUAGGG 
                   
               
               
                   
                 CUCCUGGCGUGCCUGCUAUGCAGCCUCAGUACUUCGACCU 
                   
               
               
                   
                 ACCGCUGAUCCAGCCCAUCAGUCAAGGUGCCCCAGUGGCC 
                   
               
               
                   
                 CCACUGAGGGCUAGCAUGGGCCCGGUCCCUCCUGUUCCAG 
                   
               
               
                   
                 CCACCCAGCCUCAAUAUUUCGACAUUCCUCUGACCGAGCC 
                   
               
               
                   
                 UAUAAACCAGGGCGCGUCUGCGGCCCACUUCCUGCCUCAA 
                   
               
               
                   
                 CAGCCAAUGGAAGGCCCGCUGGUCCCAGAACAGUGGAUG 
                   
               
               
                   
                 UUCCCUGGAGCUGCCCUGAGCCAAUCAGUGAGGCCGGGA 
                   
               
               
                   
                 GUUGCCCAGAGCCAGUAUUUCGAUCUCCCUUUGACACAG 
                   
               
               
                   
                 CCUAUCAAUCACGGAGCACCUGCCGCUCACUUCCUUCACC 
                   
               
               
                   
                 AGCCUCCGAUGGAAGGUCCUUGGGUUCCAGAGCAAUGGA 
                   
               
               
                   
                 UGUUCCAAGGUGCGCCACCAUCUCAGGGCACAGACGUGG 
                   
               
               
                   
                 UCCAGCAUCAGCUGGACGCGCUUGGCUACACUCUACACG 
                   
               
               
                   
                 GCCUGAACCACCCGGGCGUUCCAGUGAGUCCAGCCGUGA 
                   
               
               
                   
                 ACCAGUACCAUCUCUCUCAGGCUGCCUUCGGCCUGCCCAU 
                   
               
               
                   
                 UGACGAGGACGAGAGCGGCGAGGGCAGCGAUACCAGCGA 
                   
               
               
                   
                 GCCCUGCGAAGCCCUGGAUCUUUCCAUCCACGGCCGACCU 
                   
               
               
                   
                 UGCCCACAGGCUCCAGAGUGGCCCGUACAGGAAGAAGGC 
                   
               
               
                   
                 GGCCAGGACGCCACCGAGGUCCUGGACUUGUCCAUACAU 
                   
               
               
                   
                 GGCAGACCGCGCCCUAGGACCCCGGAGUGGCCAGUUCAG 
                   
               
               
                   
                 GGUGAAGGUGGUCAGAACGUGACCGGCCCGGAAACCCGA 
                   
               
               
                   
                 AGGGUGGUCGUGUCCGCUGUUGUCCACAUGUGUCAGGAU 
                   
               
               
                   
                 GACGAAUUCCCAGAUCUGCAAGAUCCACCCGACGAGGCC 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MDKDRPGPPALDDNMEEEVPSTSVVQEQVSAGDWENVLIELS 
                  43 
               
               
                 amino acid 
                 DSSSEKEAEDAHLEPAQKGTKRKRVDHDAGGSAPARPMLPPQ 
                   
               
               
                 sequence 
                 PDLPGREAILRRFPLDLRTLLQAIGAAATRIDTRAIDQFFGSQIS 
                   
               
               
                   
                 NTEMYIMYAMAIRQAIRDRRRNPASRRDQAKWRLQTLAAGW 
                   
               
               
                   
                 PMGYQAYSSWMYSYTDHQTTPTFVHLQATLGCTGGRRCHVT 
                   
               
               
                   
                 FSAGTFKLPRCTPGDRQWLYVQSSVGNIVQSCNPRYSIFFDYM 
                   
               
               
                   
                 AIHRSLTKIWEEVLTPDQRVSFMEFLGFLQRTDLSYIKSFVSDA 
                   
               
               
                   
                 LGTTSIQTPWIDDNPSTETAQAWNAGFLRGRAYGIDLLRTEGE 
                   
               
               
                   
                 HVEGATGETREESEDTESDGDDEDLPCIVSRGGPKVKRPPIFIR 
                   
               
               
                   
                 RLHRLLLMRAGKRTEQGKEVLEKARGSTYGTPRPPVPKPRPE 
                   
               
               
                   
                 VPQSDETATSHGSAQVPEPPTIHLAAQGMAYPLHEQHGMAPC 
                   
               
               
                   
                 PVAQAPPTPLPPVSPGDQLPGVFSDGRVACAPVPAPAGPIVRP 
                   
               
               
                   
                 WEPSLTQAAGQAFAPVRPQHMPVEPVPVPTVALERPVYPKPV 
                   
               
               
                   
                 RPAPPKIAMQGPGETSGIRRARERWRPAPWTPNPPRSPSQMSV 
                   
               
               
                   
                 RDRLARLRAEAQVKQASVEVQPPQLTQVSPQQPMEGPLVPEQ 
                   
               
               
                   
                 QMFPGAPFSQVADVVRAPGVPAMQPQYFDLPLIQPISQGAPV 
                   
               
               
                   
                 APLRASMGPVPPVPATQPQYFDIPLTEPINQGASAAHFLPQQP 
                   
               
               
                   
                 MEGPLVPEQWMFPGAALSQSVRPGVAQSQYFDLPLTQPINHG 
                   
               
               
                   
                 APAAHFLHQPPMEGPWVPEQWMFQGAPPSQGTDVVQHQLD 
                   
               
               
                   
                 ALGYTLHGLNHPGVPVSPAVNQYHLSQAAFGLPIDEDESGEG 
                   
               
               
                   
                 SDTSEPCEALDLSIHGRPCPQAPEWPVQEEGGQDATEVLDLSI 
                   
               
               
                   
                 HGRPRPRTPEWPVQGEGGQNVTGPETRRVVVSAVVHMCQDD 
                   
               
               
                   
                 EFPDLQDPPDEA 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350 A44Y V10 Stab 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 162 consists of from 5′ end to 3′ end: 
                 162 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 44, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  44 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUUACGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTY 
                  45 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350 1150F V11 Stab 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 163 consists of from 5′ end to 3′ end: 
                 163 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 46, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  46 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAUUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  47 
               
               
                 amino acid 
                 DVNVTFNFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350 M2021 V12 Stab 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 164 consists of from 5′ end to 3′ end: 
                 164 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 48, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  48 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUACUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  49 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEILGNEIDIECIME 
                   
               
               
                   
                 DGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGTG 
                   
               
               
                   
                 YAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQSNI 
                   
               
               
                   
                 VFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSPNV 
                   
               
               
                   
                 TVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASNRT 
                   
               
               
                   
                 FDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTSPT 
                   
               
               
                   
                 LNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTSPT 
                   
               
               
                   
                 PAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPNA 
                   
               
               
                   
                 TSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVTTP 
                   
               
               
                   
                 TPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATSAV 
                   
               
               
                   
                 TTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTSAH 
                   
               
               
                   
                 PTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSSTST 
                   
               
               
                   
                 KPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKANST 
                   
               
               
                   
                 TGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPPSTS 
                   
               
               
                   
                 SKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQWAS 
                   
               
               
                   
                 LAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350 11417W V13 Stab 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 165 consists of from 5′ end to 3′ end: 
                 165 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 50, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  50 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCUGGAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  51 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTWKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350 V14 Stab4 mut 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 166 consists of from 5′ end to 3′ end: 
                 166 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 52, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  52 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUUACGACGUGAACGUGACAUUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUACUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCUGGAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTY 
                  53 
               
               
                 amino acid 
                 DVNVTFNFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEILGNEIDIECIME 
                   
               
               
                   
                 DGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGTG 
                   
               
               
                   
                 YAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQSNI 
                   
               
               
                   
                 VFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSPNV 
                   
               
               
                   
                 TVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASNRT 
                   
               
               
                   
                 FDITVSGLGTAPKTLIITRTATNATTTTWKVIFSKAPESTTTSPT 
                   
               
               
                   
                 LNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTSPT 
                   
               
               
                   
                 PAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPNA 
                   
               
               
                   
                 TSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVTTP 
                   
               
               
                   
                 TPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATSAV 
                   
               
               
                   
                 TTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTSAH 
                   
               
               
                   
                 PTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSSTST 
                   
               
               
                   
                 KPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKANST 
                   
               
               
                   
                 TGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPPSTS 
                   
               
               
                   
                 SKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQWAS 
                   
               
               
                   
                 LAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350 V15 Stab4_NGM6 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 167 consists of from 5′ end to 3′ end: 
                 167 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 54, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  54 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUUACGACGUGAACGUGACAUUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUGCGAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAGCGAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 GCGGUCAAGACCGAGAUACUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCGCGUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUGCGGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCGCGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCUGG 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTY 
                  55 
               
               
                 amino acid 
                 DVNVTFNFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENAANLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSAN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFAVKTEILGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNIACSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVAVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNAATTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTWDVT 
                   
               
               
                   
                 SPTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTP 
                   
               
               
                   
                 NATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAV 
                   
               
               
                   
                 TTPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNAT 
                   
               
               
                   
                 SAVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLT 
                   
               
               
                   
                 SAHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNS 
                   
               
               
                   
                 STSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGK 
                   
               
               
                   
                 ANSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYL 
                   
               
               
                   
                 PPSTSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVL 
                   
               
               
                   
                 QWASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETY 
                   
               
               
                   
                 V 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V1_NGM 36 mut_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 168 consists of from 5′ end to 3′ end: 
                 168 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 56, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  56 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGGCGAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUGCGAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUGCGACCG 
                   
               
               
                   
                 GGGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGU 
                   
               
               
                   
                 ACUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACG 
                   
               
               
                   
                 CUGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUG 
                   
               
               
                   
                 UACCCUACAUCAAGUGGGAUAAUUGCAAUUCAGCGAAUA 
                   
               
               
                   
                 UUGCGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUC 
                   
               
               
                   
                 UGCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUU 
                   
               
               
                   
                 CGCGGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAU 
                   
               
               
                   
                 CGAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCU 
                   
               
               
                   
                 GCCCGGCGACAACAAGUUCAAUAUCGCGUGUUCUGGCUA 
                   
               
               
                   
                 CGAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUAC 
                   
               
               
                   
                 UUCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCC 
                   
               
               
                   
                 UACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCC 
                   
               
               
                   
                 UGGGUAACAAUGCGAUCUUAUACGUGUUUUAUAGCGGAA 
                   
               
               
                   
                 ACGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGA 
                   
               
               
                   
                 GUAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGG 
                   
               
               
                   
                 ACAUGCCGACAAACACAGCGGAUAUUACCUACGUGGGCG 
                   
               
               
                   
                 ACAAUGCCGCGUAUUCAGUGCCCAUGGUCACGAGCGAGG 
                   
               
               
                   
                 ACGCCAAUUCACCAAAUGUUGCGGUAACAGCUUUCUGGG 
                   
               
               
                   
                 CCUGGCCAAAUAACGCGGAGACUGACUUCAAAUGUAAGU 
                   
               
               
                   
                 GGACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUA 
                   
               
               
                   
                 UCGCGGGGGCCUUUGCUUCCAACAGGGCGUUCGACAUCA 
                   
               
               
                   
                 CUGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCA 
                   
               
               
                   
                 UAACACGGACCGCCACCAACGCCGCGACUACAACCCAUAA 
                   
               
               
                   
                 GGUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCC 
                   
               
               
                   
                 CCGACUCUUAACACUGCGGGCUUCGCUGAUCCCAACACCG 
                   
               
               
                   
                 CGACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACC 
                   
               
               
                   
                 UGGCGGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGC 
                   
               
               
                   
                 CGAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAGCGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAGCGAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGGCGUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCGCGAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAGCGGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGGCGAAUCACGCGUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUGCGUCCGCUGUCACUACCGGACAGCACAACAUCGCGA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUGC 
                   
               
               
                   
                 GUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCGCGCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCGCGA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGGCGAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAGCGAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAGCGACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 GCGACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUGCGAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCGCGACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  57 
               
               
                 amino acid 
                 DVNVAINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENAANLFLLELLGAGELALTMRSKKLPINVATGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSAN 
                   
               
               
                   
                 IAAVVRAQGLDVTLPLSLPTSAQDSNFAVKTEMLGNEIDIECI 
                   
               
               
                   
                 MEDGEISQVLPGDNKFNIACSGYESHVPSGGILTSTSPVATPIP 
                   
               
               
                   
                 GTGYAYSLRLTPRPVSRFLGNNAILYVFYSGNGPKASGGDYCI 
                   
               
               
                   
                 QSNIVFSDEIPASQDMPTNTADITYVGDNAAYSVPMVTSEDAN 
                   
               
               
                   
                 SPNVAVTAFWAWPNNAETDFKCKWTLTSGTPSGCENIAGAF 
                   
               
               
                   
                 ASNRAFDITVSGLGTAPKTLIITRTATNAATTTHKVIFSKAPEST 
                   
               
               
                   
                 TTSPTLNTAGFADPNTATGLPSSTHVPTNLAAPASTGPTVSTA 
                   
               
               
                   
                 DVTSPTPAGTTSGASPVTPSPSPWDNGAESKAPDMTSSTSPVT 
                   
               
               
                   
                 TPTPNAASPTPAVTTPTPNAASPTPAVTTPTPNAASPTLGKTSP 
                   
               
               
                   
                 TSAVTTPTPNAAGPTVGETSPQANAANHALGGTSPTPVVTSQP 
                   
               
               
                   
                 KNAASAVTTGQHNIASSSTSSMSLRPSSNPETLSPSTSDNSASH 
                   
               
               
                   
                 MPLLTSAHPTGGENIAQVTPASISTHHVSTSSPAPRPGTTSQAS 
                   
               
               
                   
                 GPGNSATSTKPGEVNVAKGTPPQNAASPQAPSGQKTAVPTVT 
                   
               
               
                   
                 STGGKANSATGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYN 
                   
               
               
                   
                 AATYLPPSTSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNL 
                   
               
               
                   
                 AMLVLQWASLAVLTLLLLLVMADCAFRRNLATSHTYTTPPY 
                   
               
               
                   
                 DDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V2_NGM 18 mut_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 169 consists of from 5′ end to 3′ end: 
                 169 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 58, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  58 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGGCGAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUGCGAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUGCGACCG 
                   
               
               
                   
                 GGGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGU 
                   
               
               
                   
                 ACUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACG 
                   
               
               
                   
                 CUGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUG 
                   
               
               
                   
                 UACCCUACAUCAAGUGGGAUAAUUGCAAUUCAGCGAAUA 
                   
               
               
                   
                 UUGCGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUC 
                   
               
               
                   
                 UGCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUU 
                   
               
               
                   
                 CGCGGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAU 
                   
               
               
                   
                 CGAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCU 
                   
               
               
                   
                 GCCCGGCGACAACAAGUUCAAUAUCGCGUGUUCUGGCUA 
                   
               
               
                   
                 CGAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUAC 
                   
               
               
                   
                 UUCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCC 
                   
               
               
                   
                 UACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCC 
                   
               
               
                   
                 UGGGUAACAAUGCGAUCUUAUACGUGUUUUAUAGCGGAA 
                   
               
               
                   
                 ACGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGA 
                   
               
               
                   
                 GUAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGG 
                   
               
               
                   
                 ACAUGCCGACAAACACAGCGGAUAUUACCUACGUGGGCG 
                   
               
               
                   
                 ACAAUGCCGCGUAUUCAGUGCCCAUGGUCACGAGCGAGG 
                   
               
               
                   
                 ACGCCAAUUCACCAAAUGUUGCGGUAACAGCUUUCUGGG 
                   
               
               
                   
                 CCUGGCCAAAUAACGCGGAGACUGACUUCAAAUGUAAGU 
                   
               
               
                   
                 GGACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUA 
                   
               
               
                   
                 UCGCGGGGGCCUUUGCUUCCAACAGGGCGUUCGACAUCA 
                   
               
               
                   
                 CUGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCA 
                   
               
               
                   
                 UAACACGGACCGCCACCAACGCCGCGACUACAACCCAUAA 
                   
               
               
                   
                 GGUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCC 
                   
               
               
                   
                 CCGACUCUUAACACUGCGGGCUUCGCUGAUCCCAACACCG 
                   
               
               
                   
                 CGACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACC 
                   
               
               
                   
                 UGGCGGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGC 
                   
               
               
                   
                 CGAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  59 
               
               
                 amino acid 
                 DVNVAINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENAANLFLLELLGAGELALTMRSKKLPINVATGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSAN 
                   
               
               
                   
                 IAAVVRAQGLDVTLPLSLPTSAQDSNFAVKTEMLGNEIDIECI 
                   
               
               
                   
                 MEDGEISQVLPGDNKFNIACSGYESHVPSGGILTSTSPVATPIP 
                   
               
               
                   
                 GTGYAYSLRLTPRPVSRFLGNNAILYVFYSGNGPKASGGDYCI 
                   
               
               
                   
                 QSNIVFSDEIPASQDMPTNTADITYVGDNAAYSVPMVTSEDAN 
                   
               
               
                   
                 SPNVAVTAFWAWPNNAETDFKCKWTLTSGTPSGCENIAGAF 
                   
               
               
                   
                 ASNRAFDITVSGLGTAPKTLIITRTATNAATTTHKVIFSKAPEST 
                   
               
               
                   
                 TTSPTLNTAGFADPNTATGLPSSTHVPTNLAAPASTGPTVSTA 
                   
               
               
                   
                 DVTSPTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVT 
                   
               
               
                   
                 TPTPNATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPT 
                   
               
               
                   
                 SAVTTPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPK 
                   
               
               
                   
                 NATSAVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHM 
                   
               
               
                   
                 PLLTSAHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGP 
                   
               
               
                   
                 GNSSTSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTG 
                   
               
               
                   
                 GKANSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATT 
                   
               
               
                   
                 YLPPSTSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSML 
                   
               
               
                   
                 VLQWASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAE 
                   
               
               
                   
                 TYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V4_NGM N87_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 170 consists of from 5′ end to 3′ end: 
                 170 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 60, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  60 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUGCGAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  61 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENAANLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V5_NGM N166_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 171 consists of from 5′ end to 3′ end: 
                 171 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 62, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  62 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAGCGAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  63 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSAN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V6_NGM N195_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 172 consists of from 5′ end to 3′ end: 
                 172 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 64, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  64 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 GCGGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  65 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFAVKTEMLGNEIDIECI 
                   
               
               
                   
                 MEDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPG 
                   
               
               
                   
                 TGYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQ 
                   
               
               
                   
                 SNIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANS 
                   
               
               
                   
                 PNVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFAS 
                   
               
               
                   
                 NRTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTT 
                   
               
               
                   
                 SPTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVT 
                   
               
               
                   
                 SPTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTP 
                   
               
               
                   
                 NATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAV 
                   
               
               
                   
                 TTPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNAT 
                   
               
               
                   
                 SAVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLT 
                   
               
               
                   
                 SAHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNS 
                   
               
               
                   
                 STSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGK 
                   
               
               
                   
                 ANSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYL 
                   
               
               
                   
                 PPSTSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVL 
                   
               
               
                   
                 QWASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETY 
                   
               
               
                   
                 V 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V7_NGM N229_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 173 consists of from 5′ end to 3′ end: 
                 173 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 66, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  66 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCGCGUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  67 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNIACSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V8_NGM N345_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 174 consists of from 5′ end to 3′ end: 
                 174 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 68, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  68 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUGCGGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                 114 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVAVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V9_NGM N411_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 175 consists of from 5′ end to 3′ end: 
                 175 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 69, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  69 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCGCGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  70 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNAATTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp42 (B95-8)_DX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 176 consists of from 5′ end to 3′ end: 
                 176 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 70, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGUGAGCUUCAAGCAGGUGAGAGUGCCCCUGUUCACC 
                  71 
               
               
                 Construct 
                 GCCAUCGCCCUGGUGAUCGUGCUGCUGCUGGCCUACUUCC 
                   
               
               
                 (excluding 
                 UGCCCCCCAGAGUGAGAGGCGGCGGCAGAGUGGCCGCCG 
                   
               
               
                 the stop 
                 CCGCCAUCACCUGGGUGCCCAAGCCCAACGUGGAGGUGU 
                   
               
               
                 codon) 
                 GGCCCGUGGACCCCCCACCCCCCGUGAACUUCAACAAGAC 
                   
               
               
                   
                 CGCCGAGCAGGAGUACGGCGACAAGGAGGUGAAGCUGCC 
                   
               
               
                   
                 CCACUGGACCCCCACCCUGCACACCUUCCAGGUGCCCCAG 
                   
               
               
                   
                 AACUACACCAAGGCCAACUGCACCUACUGCAACACCAGAG 
                   
               
               
                   
                 AGUACACCUUCAGCUACAAGGGCUGCUGCUUCUACUUCA 
                   
               
               
                   
                 CCAAGAAGAAGCACACCUGGAACGGCUGCUUCCAGGCCU 
                   
               
               
                   
                 GCGCCGAGCUGUACCCCUGCACCUACUUCUACGGCCCCAC 
                   
               
               
                   
                 CCCCGACAUCCUGCCCGUGGUGACCAGAAACCUGAACGCC 
                   
               
               
                   
                 AUCGAGAGCCUGUGGGUGGGCGUGUACAGAGUGGGCGAG 
                   
               
               
                   
                 GGCAACUGGACCAGCCUGGACGGCGGCACCUUCAAGGUG 
                   
               
               
                   
                 UACCAGAUCUUCGGCAGCCACUGCACCUACGUGAGCAAG 
                   
               
               
                   
                 UUCAGCACCGUGCCCGUGAGCCACCACGAGUGCAGCUUCC 
                   
               
               
                   
                 UGAAGCCCUGCCUGUGCGUGAGCCAGAGAAGCAACAGC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MVSFKQVRVPLFTAIALVIVLLLAYFLPPRVRGGGRVAAAAIT 
                  34 
               
               
                 amino acid 
                 WVPKPNVEVWPVDPPPPVNFNKTAEQEYGDKEVKLPHWTPT 
                   
               
               
                 sequence 
                 LHTFQVPQNYTKANCTYCNTREYTFSYKGCCFYFTKKKHTW 
                   
               
               
                   
                 NGCFQACAELYPCTYFYGPTPDILPVVTRNLNAIESLWVGVYR 
                   
               
               
                   
                 VGEGNWTSLDGGTFKVYQIFGSHCTYVSKFSTVPVSHHECSFL 
                   
               
               
                   
                 KPCLCVSQRSNS 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV EBNA3 (B95-8)_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 177 consists of from 5′ end to 3′ end: 
                 177 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 72, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGACAAGGACAGGCCCGGCCCUCCCGCCUUGGACGACA 
                  72 
               
               
                 Construct 
                 ACAUGGAGGAGGAGGUGCCCAGCACCAGCGUGGUGCAGG 
                   
               
               
                 (excluding 
                 AGCAGGUGAGCGCCGGCGACUGGGAGAACGUGUUAAUCG 
                   
               
               
                 the stop 
                 AGUUGAGCGACAGCAGCAGCGAGAAGGAGGCCGAGGACG 
                   
               
               
                 codon) 
                 CCCACCUGGAGCCCGCCCAGAAGGGCACCAAGAGGAAGA 
                   
               
               
                   
                 GGGUGGACCACGACGCUGGCGGAUCUGCCCCUGCCAGGCC 
                   
               
               
                   
                 CAUGCUGCCACCCCAGCCCGACCUGCCCGGCAGGGAGGCC 
                   
               
               
                   
                 AUCCUGAGGAGGUUCCCACUGGACCUGAGGACCCUGCUG 
                   
               
               
                   
                 CAGGCCAUCGGCGCCGCCGCCACCAGGAUCGACACCAGGG 
                   
               
               
                   
                 CCAUCGACCAGUUCUUCGGCAGCCAGAUCAGCAACACCGA 
                   
               
               
                   
                 GAUGUACAUCAUGUACGCCAUGGCCAUCAGACAAGCAAU 
                   
               
               
                   
                 UAGGGAUCGACGCAGAAAUCCUGCCAGCAGGAGGGACCA 
                   
               
               
                   
                 GGCCAAGUGGAGGCUGCAGACCCUGGCCGCCGGCUGGCCC 
                   
               
               
                   
                 AUGGGCUACCAGGCCUACAGCAGCUGGAUGUACAGCUAC 
                   
               
               
                   
                 ACCGACCACCAGACCACUCCGACCUUCGUGCACCUCCAGG 
                   
               
               
                   
                 CUACCCUGGGCUGCACUGGCGGUCGCAGGUGCCACGUGA 
                   
               
               
                   
                 CCUUCUCAGCGGGCACCUUCAAGCUGCCCAGGUGCACACC 
                   
               
               
                   
                 UGGCGACAGGCAGUGGCUGUACGUGCAGAGCAGCGUGGG 
                   
               
               
                   
                 CAACAUUGUUCAGAGUUGCAACCCCAGGUACAGCAUCUU 
                   
               
               
                   
                 CUUCGACUACAUGGCGAUUCACAGGAGCCUGACCAAGAU 
                   
               
               
                   
                 CUGGGAGGAAGUCCUGACCCCUGACCAGAGGGUGAGCUU 
                   
               
               
                   
                 CAUGGAGUUCCUGGGCUUCCUGCAGAGGACCGACCUGAG 
                   
               
               
                   
                 CUACAUCAAGAGCUUCGUGAGCGACGCCCUGGGCACCACC 
                   
               
               
                   
                 AGCAUCCAGACUCCUUGGAUCGACGAUAACCCUAGCACC 
                   
               
               
                   
                 GAAACCGCCCAGGCCUGGAACGCCGGUUUCCUGAGGGGC 
                   
               
               
                   
                 AGGGCCUACGGCAUCGACCUGCUCCGGACCGAGGGCGAG 
                   
               
               
                   
                 CACGUGGAGGGCGCCACCGGCGAGACUAGGGAGGAGAGC 
                   
               
               
                   
                 GAGGACACUGAGAGCGACGGCGACGACGAGGAUCUGCCG 
                   
               
               
                   
                 UGCAUCGUGAGCAGGGGCGGCCCCAAGGUGAAGAGGCCU 
                   
               
               
                   
                 CCCAUCUUCAUCCGGAGGCUUCACAGGCUGCUGCUGAUG 
                   
               
               
                   
                 AGGGCCGGCAAGCGUACUGAACAGGGCAAGGAGGUACUG 
                   
               
               
                   
                 GAGAAGGCCAGGGGCAGCACAUAUGGCACCCCUAGACCG 
                   
               
               
                   
                 CCUGUGCCCAAGCCGCGUCCUGAAGUCCCGCAGAGUGAU 
                   
               
               
                   
                 GAGACAGCGACUAGCCAUGGCAGUGCUCAGGUGCCCGAG 
                   
               
               
                   
                 CCACCCACCAUCCAUCUCGCCGCACAGGGCAUGGCCUACC 
                   
               
               
                   
                 CACUGCACGAGCAGCACGGCAUGGCGCCCUGCCCCGUGGC 
                   
               
               
                   
                 CCAGGCUCCUCCAACCCCGCUUCCUCCAGUAUCCCCUGGU 
                   
               
               
                   
                 GACCAGUUGCCAGGCGUGUUCAGCGACGGUAGGGUGGCC 
                   
               
               
                   
                 UGCGCGCCAGUUCCUGCACCAGCAGGCCCAAUUGUGAGG 
                   
               
               
                   
                 CCCUGGGAGCCCUCCCUAACCCAGGCGGCAGGUCAGGCCU 
                   
               
               
                   
                 UCGCUCCAGUUAGACCACAGCACAUGCCCGUGGAACCGG 
                   
               
               
                   
                 UGCCAGUGCCUACGGUGGCGCUGGAGAGGCCCGUGUACC 
                   
               
               
                   
                 CAAAGCCUGUGCGACCUGCGCCUCCUAAGAUCGCCAUGCA 
                   
               
               
                   
                 AGGCCCGGGCGAGACUAGCGGUAUCAGGCGGGCCAGGGA 
                   
               
               
                   
                 GAGGUGGCGCCCGGCACCGUGGACUCCCAACCCACCAAGG 
                   
               
               
                   
                 UCACCUAGUCAGAUGAGCGUGCGUGAUAGGCUGGCACGG 
                   
               
               
                   
                 CUUCGGGCAGAGGCUCAGGUAAAGCAGGCCAGUGUGGAG 
                   
               
               
                   
                 GUGCAGCCACCACAGCUGACUCAGGUGUCCCCGCAGCAGC 
                   
               
               
                   
                 CCAUGGAGGGCCCACUGGUGCCGGAACAGCAGAUGUUCC 
                   
               
               
                   
                 CCGGCGCCCCGUUCAGCCAGGUGGCCGACGUGGUUAGGG 
                   
               
               
                   
                 CUCCUGGCGUGCCUGCUAUGCAGCCUCAGUACUUCGACCU 
                   
               
               
                   
                 ACCGCUGAUCCAGCCCAUCAGUCAAGGUGCCCCAGUGGCC 
                   
               
               
                   
                 CCACUGAGGGCUAGCAUGGGCCCGGUCCCUCCUGUUCCAG 
                   
               
               
                   
                 CCACCCAGCCUCAAUAUUUCGACAUUCCUCUGACCGAGCC 
                   
               
               
                   
                 UAUAAACCAGGGCGCGUCUGCGGCCCACUUCCUGCCUCAA 
                   
               
               
                   
                 CAGCCAAUGGAAGGCCCGCUGGUCCCAGAACAGUGGAUG 
                   
               
               
                   
                 UUCCCUGGAGCUGCCCUGAGCCAAUCAGUGAGGCCGGGA 
                   
               
               
                   
                 GUUGCCCAGAGCCAGUAUUUCGAUCUCCCUUUGACACAG 
                   
               
               
                   
                 CCUAUCAAUCACGGAGCACCUGCCGCUCACUUCCUUCACC 
                   
               
               
                   
                 AGCCUCCGAUGGAAGGUCCUUGGGUUCCAGAGCAAUGGA 
                   
               
               
                   
                 UGUUCCAAGGUGCGCCACCAUCUCAGGGCACAGACGUGG 
                   
               
               
                   
                 UCCAGCAUCAGCUGGACGCGCUUGGCUACACUCUACACG 
                   
               
               
                   
                 GCCUGAACCACCCGGGCGUUCCAGUGAGUCCAGCCGUGA 
                   
               
               
                   
                 ACCAGUACCAUCUCUCUCAGGCUGCCUUCGGCCUGCCCAU 
                   
               
               
                   
                 UGACGAGGACGAGAGCGGCGAGGGCAGCGAUACCAGCGA 
                   
               
               
                   
                 GCCCUGCGAAGCCCUGGAUCUUUCCAUCCACGGCCGACCU 
                   
               
               
                   
                 UGCCCACAGGCUCCAGAGUGGCCCGUACAGGAAGAAGGC 
                   
               
               
                   
                 GGCCAGGACGCCACCGAGGUCCUGGACUUGUCCAUACAU 
                   
               
               
                   
                 GGCAGACCGCGCCCUAGGACCCCGGAGUGGCCAGUUCAG 
                   
               
               
                   
                 GGUGAAGGUGGUCAGAACGUGACCGGCCCGGAAACCCGA 
                   
               
               
                   
                 AGGGUGGUCGUGUCCGCUGUUGUCCACAUGUGUCAGGAU 
                   
               
               
                   
                 GACGAAUUCCCAGAUCUGCAAGAUCCACCCGACGAGGCC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MDKDRPGPPALDDNMEEEVPSTSVVQEQVSAGDWENVLIELS 
                  43 
               
               
                 amino acid 
                 DSSSEKEAEDAHLEPAQKGTKRKRVDHDAGGSAPARPMLPPQ 
                   
               
               
                 sequence 
                 PDLPGREAILRRFPLDLRTLLQAIGAAATRIDTRAIDQFFGSQIS 
                   
               
               
                   
                 NTEMYIMYAMAIRQAIRDRRRNPASRRDQAKWRLQTLAAGW 
                   
               
               
                   
                 PMGYQAYSSWMYSYTDHQTTPTFVHLQATLGCTGGRRCHVT 
                   
               
               
                   
                 FSAGTFKLPRCTPGDRQWLYVQSSVGNIVQSCNPRYSIFFDYM 
                   
               
               
                   
                 AIHRSLTKIWEEVLTPDQRVSFMEFLGFLQRTDLSYIKSFVSDA 
                   
               
               
                   
                 LGTTSIQTPWIDDNPSTETAQAWNAGFLRGRAYGIDLLRTEGE 
                   
               
               
                   
                 HVEGATGETREESEDTESDGDDEDLPCIVSRGGPKVKRPPIFIR 
                   
               
               
                   
                 RLHRLLLMRAGKRTEQGKEVLEKARGSTYGTPRPPVPKPRPE 
                   
               
               
                   
                 VPQSDETATSHGSAQVPEPPTIHLAAQGMAYPLHEQHGMAPC 
                   
               
               
                   
                 PVAQAPPTPLPPVSPGDQLPGVFSDGRVACAPVPAPAGPIVRP 
                   
               
               
                   
                 WEPSLTQAAGQAFAPVRPQHMPVEPVPVPTVALERPVYPKPV 
                   
               
               
                   
                 RPAPPKIAMQGPGETSGIRRARERWRPAPWTPNPPRSPSQMSV 
                   
               
               
                   
                 RDRLARLRAEAQVKQASVEVQPPQLTQVSPQQPMEGPLVPEQ 
                   
               
               
                   
                 QMFPGAPFSQVADVVRAPGVPAMQPQYFDLPLIQPISQGAPV 
                   
               
               
                   
                 APLRASMGPVPPVPATQPQYFDIPLTEPINQGASAAHFLPQQP 
                   
               
               
                   
                 MEGPLVPEQWMFPGAALSQSVRPGVAQSQYFDLPLTQPINHG 
                   
               
               
                   
                 APAAHFLHQPPMEGPWVPEQWMFQGAPPSQGTDVVQHQLD 
                   
               
               
                   
                 ALGYTLHGLNHPGVPVSPAVNQYHLSQAAFGLPIDEDESGEG 
                   
               
               
                   
                 SDTSEPCEALDLSIHGRPCPQAPEWPVQEEGGQDATEVLDLSI 
                   
               
               
                   
                 HGRPRPRTPEWPVQGEGGQNVTGPETRRVVVSAVVHMCQDD 
                   
               
               
                   
                 EFPDLQDPPDEA 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBNA1_trunc_001 (modified) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 178 consists of from 5′ end to 3′ end: 
                 178 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 73, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGCAGAAGACCCUUCUUUCACCCCGUGGGAGAAGCC 
                  73 
               
               
                 Construct 
                 GACUAUUUCGAGUACCACCAGGAGGGCGGUCCUGACGGC 
                   
               
               
                 (excluding 
                 GAGCCCGACGUGCCUCCAGGCGCCAUCGAACAGGGCCCCG 
                   
               
               
                 the stop 
                 CUGACGAUCCUGGCGAGGGGCCUUCCACAGGCCCUCGCGG 
                   
               
               
                 codon) 
                 CCAGGGAGAUGGCGGAAGGCGGAAGAAGGGCGGCUGGUU 
                   
               
               
                   
                 UGGCAAGCAUAGGGGCCAGGGCGGCUCCAAUCCCAAGUU 
                   
               
               
                   
                 CGAGAAUAUCGCCGAGGGGCUCCGGGCUCUGCUGGCCCG 
                   
               
               
                   
                 GUCGCACGUCGAACGUACCACUGACGAGGGGACGUGGGU 
                   
               
               
                   
                 GGCCGGCGUCUUUGUGUACGGAGGAUCCAAGACCUCCCU 
                   
               
               
                   
                 GUACAACCUGAGAAGGGGCACCGCCCUUGCCAUCCCUCAG 
                   
               
               
                   
                 UGCAGACUCACGCCCCUGAGCAGGCUCCCCUUUGGGAUG 
                   
               
               
                   
                 GCUCCUGGGCCAGGCCCGCAGCCCGGGCCCCUCAGGGAAA 
                   
               
               
                   
                 GCAUCGUGUGUUACUUUAUGGUGUUUCUGCAGACACACA 
                   
               
               
                   
                 UCUUUGCAGAAGUUCUGAAAGAUGCAAUCAAGGAUCUGG 
                   
               
               
                   
                 UGAUGACCAAGCCUGCACCUACGUGCAAUAUCAGGGUGA 
                   
               
               
                   
                 CAGUAUGCUCCUUCGACGACGGUGUGGACCUCCCGCCCUG 
                   
               
               
                   
                 GUUCCCACCCAUGGUCGAGGGUGCCGCCGCCGAGGGAGA 
                   
               
               
                   
                 CGAUGGCGACGACGGUGAUGAGGGUGGAGACGGAGAUGA 
                   
               
               
                   
                 AGGCGAGGAGGGGCAGGAG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADD 
                  10 
               
               
                 amino acid 
                 PGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIA 
                   
               
               
                 sequence 
                 EGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRG 
                   
               
               
                   
                 TALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQ 
                   
               
               
                   
                 THIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWF 
                   
               
               
                   
                 PPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV LMP1_DX_(modified)_DX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 179 consists of from 5′ end to 3′ end: 
                 179 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 74, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAACACGACCUUGAGAGGGGCCCACCGGGCCCGCGA 
                  74 
               
               
                 Construct 
                 CGGCCACCUCGAGGACCUCCCCUCUCCUCUUCCCUAGGCC 
                   
               
               
                 (excluding 
                 UUGCUCUCCUUCUCCUCCUCUUGGCGCUACUGUUUUGGC 
                   
               
               
                 the stop 
                 UGUACAUCGUUAUGAGUGACUGGACUGGAGGAGCCCUCC 
                   
               
               
                 codon) 
                 UUGUCCUCUAUUCCUUUGCUCUCAUGCUUAUAAUUAUAA 
                   
               
               
                   
                 UUUUGAUCAUCUUUAUCUUCAGAAGAGACCUUCUCUGUC 
                   
               
               
                   
                 CACUUGGAGCCCUUUGUAUACUCCUACUGAUGAUCACCC 
                   
               
               
                   
                 UCCUGCUCAUCGCUCUCUGGAAUUUGCACGGACAGGCAU 
                   
               
               
                   
                 UGUUCCUUGGAAUUGUGCUGUUCAUCUUCGGGUGCUUAC 
                   
               
               
                   
                 UUGUCUUAGGUAUCUGGAUCUACUUAUUGGAGAUGCUCU 
                   
               
               
                   
                 GGCGACUUGGUGCCACCAUCUGGCAGCUUUUGGCCUUCU 
                   
               
               
                   
                 UCCUAGCCUUCUUCCUAGACCUCAUCCUGCUCAUUAUUGC 
                   
               
               
                   
                 UCUCUAUCUACAACAGAACUGGUGGACUCUAUUGGUUGA 
                   
               
               
                   
                 UCUCCUUUGGCUCCUCCUGUUUCUGGCGAUUUUAAUCUG 
                   
               
               
                   
                 GAUGUAUUACCAUGGACAACGACACAGUGAUGAACACCA 
                   
               
               
                   
                 CCACGAUGACUCCCUCCCGCACCCUCAACAAGCUACCGAU 
                   
               
               
                   
                 GAUUCUGGCCAUGAAUCUGACUCUAACUCCAACGAGGGC 
                   
               
               
                   
                 AGACACCACCUGCUCGUGAGUGGAGCCGGCGACGGACCA 
                   
               
               
                   
                 CCACUCUGCUCUCAGAACCUAGGCGCACCUGGAGGUGGU 
                   
               
               
                   
                 CCUGACAAUGGCCCACAGGACCCUGACAACACGGAUGAU 
                   
               
               
                   
                 AAUGGCCCACAGGACCCUGAUAACACUGAUGACAAUGGC 
                   
               
               
                   
                 CCACAUGACCCGCUGCCUCAAGACCCAGACAAUACUGAUG 
                   
               
               
                   
                 ACAAUGGCCCACAGGACCCUGACAACACUGAUGACAAUG 
                   
               
               
                   
                 GCCCGCACGACCCGUUACCUCAUAGCCCUAGCGACUCUGC 
                   
               
               
                   
                 UGGAAAUGAUGGAGGCCCUCCACAAUUGACGGAAGAGGU 
                   
               
               
                   
                 UGAGAACAAAGGAGGUGACCAGGGCCCGCCUUUGAUGAC 
                   
               
               
                   
                 AGACGGAGGCGGCGGUCAUAGUCAUGAUUCCGGCCAUGG 
                   
               
               
                   
                 CGGCGGUGAUCCACACCUUCCUACGCUGCUGUUGGGUUC 
                   
               
               
                   
                 UUCUGGUUCCGGUGGAGAUGAUGACGACCCGCACGGCCC 
                   
               
               
                   
                 AGUUCAGCUAAGCUACUAUGAC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEHDLERGPPGPRRPPRGPPLSSSLGLALLLLLLALLFWLYIV 
                  16 
               
               
                 amino acid 
                 MSDWTGGALLVLYSFALMLIIIILIIFIFRRDLLCPLGALCILLLM 
                   
               
               
                 sequence 
                 ITLLLIALWNLHGQALFLGIVLFIFGCLLVLGIWIYLLEMLWRL 
                   
               
               
                   
                 GATIWQLLAFFLAFFLDLILLIIALYLQQNWWTLLVDLLWLLL 
                   
               
               
                   
                 FLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSN 
                   
               
               
                   
                 SNEGRHHLLVSGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTD 
                   
               
               
                   
                 DNGPQDPDNTDDNGPHDPLPQDPDNTDDNGPQDPDNTDDNG 
                   
               
               
                   
                 PHDPLPHSPSDSAGNDGGPPQLTEEVENKGGDQGPPLMTDGG 
                   
               
               
                   
                 GGHSHDSGHGGGDPHLPTLLLGSSGSGGDDDDPHGPVQLSYY 
                   
               
               
                   
                 D 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV LMP2 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 180 consists of from 5′ end to 3′ end: 
                 180 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 75, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGGUCCCUAGAAAUGGUGCCAAUGGGCGCGGGUCCU 
                  75 
               
               
                 Construct 
                 CCUAGCCCCGGCGGGGAUCCGGAUGGGUACGAUGGCGGA 
                   
               
               
                 (excluding 
                 AACAACUCCCAAUAUCCAUCUGCUUCUGGCUCUUCUGGG 
                   
               
               
                 the stop 
                 AACACGCCCACCCCACCGAACGAUGAGGAACGUGAAUCU 
                   
               
               
                 codon) 
                 AAUGAAGAGCCACCACCGCCUUAUGAGGACCCAUAUUGG 
                   
               
               
                   
                 GGCAAUGGCGACCGUCACUCGGACUAUCAACCACUAGGA 
                   
               
               
                   
                 ACCCAAGAUCAAAGUCUGUACUUGGGAUUGCAACACGAC 
                   
               
               
                   
                 GGGAAUGACGGGCUCCCUCCACCUCCCUACUCUCCACGGG 
                   
               
               
                   
                 AUGACUCAUCUCAACACAUAUACGAAGAAGCGGGCAGAG 
                   
               
               
                   
                 GAAGUAUGAAUCCAGUAUGCCUGCCUGUAAUUGUUGCGC 
                   
               
               
                   
                 CCUACCUCUUUUGGCUGGCGGCUAUUGCCGCCUCGUGUU 
                   
               
               
                   
                 UCACGGCCUCAGUUAGUACCGUUGUGACCGCCACCGGCU 
                   
               
               
                   
                 UGGCCCUCUCACUUCUACUCUUGGCAGCAGUGGCCAGCUC 
                   
               
               
                   
                 AUAUGCCGCUGCACAAAGGAAACUGCUGACACCGGUGAC 
                   
               
               
                   
                 AGUGCUUACUGCGGUUGUCACUUUCUUUGCAAUUUGCCU 
                   
               
               
                   
                 AACAUGGAGGAUUGAGGACCCACCUUUUAAUUCUCUUCU 
                   
               
               
                   
                 GUUUGCAUUGCUGGCCGCAGCUGGCGGACUACAAGGCAU 
                   
               
               
                   
                 UUACGUUCUGGUGAUGCUUGUGCUCCUGAUACUAGCGUA 
                   
               
               
                   
                 CAGAAGGAGAUGGCGCCGUUUGACUGUUUGUGGCGGCAU 
                   
               
               
                   
                 CAUGUUCUUGGCAUGUGUACUUGUCCUCAUCGUCGACGC 
                   
               
               
                   
                 UGUUUUGCAGCUGAGUCCCCUCCUUGGAGCUGUAACUGU 
                   
               
               
                   
                 GGUUUCCAUGACGCUGCUGCUACUGGCUUUCGUCCUCUG 
                   
               
               
                   
                 GCUCUCUUCGCCAGGUGGCCUAGGUACUCUUGGUGCAGC 
                   
               
               
                   
                 CCUUUUAACAUUGGCAGCAGCUCUGGCACUGCUAGCGUC 
                   
               
               
                   
                 ACUGAUUUUGGGCACACUUAACUUGACUACAAUGUUCCU 
                   
               
               
                   
                 UCUCAUGCUCCUAUGGACACUUGUGGUUCUCCUGAUUUG 
                   
               
               
                   
                 CUCUUCGUGCUCUUCAUGUCCACUGAGCAAGAUCCUUCU 
                   
               
               
                   
                 GGCACGACUGUUCCUAUAUGCUCUCGCACUCUUGUUGCU 
                   
               
               
                   
                 AGCCUCCGCGCUAAUCGCUGGUGGCAGUAUUUUGCAAAC 
                   
               
               
                   
                 AAACUUCAAGAGUUUAAGCAGCACUGAAUUUAUACCCAA 
                   
               
               
                   
                 UUUGUUCUGCAUGUUAUUACUGAUUGUCGCUGGCAUACU 
                   
               
               
                   
                 CUUCAUUCUUGCUAUCCUGACCGAAUGGGGCAGUGGAAA 
                   
               
               
                   
                 UAGAACAUACGGUCCAGUCUUUAUGUGCCUCGGUGGCCU 
                   
               
               
                   
                 GCUCACCAUGGUAGCCGGCGCUGUGUGGCUGACGGUGAU 
                   
               
               
                   
                 GUCUAACACGCUUUUGUCUGCCUGGAUUCUUACAGCAGG 
                   
               
               
                   
                 AUUCCUGAUUUUCCUCAUUGGCUUUGCCCUCUUUGGGGU 
                   
               
               
                   
                 CAUUAGAUGCUGCCGCUACUGCUGCUACUACUGCCUUAC 
                   
               
               
                   
                 ACUGGAAAGUGAGGAGCGCCCACCGACCCCAUAUCGCAA 
                   
               
               
                   
                 CACUGUA 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNT 
                  22 
               
               
                 amino acid 
                 PTPPNDEERESNEEPPPPYEDPYWGNGDRHSDYQPLGTQDQSL 
                   
               
               
                 sequence 
                 YLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLP 
                   
               
               
                   
                 VIVAPYLFWLAAIAASCFTASVSTVVTATGLALSLLLLAAVAS 
                   
               
               
                   
                 SYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLFALL 
                   
               
               
                   
                 AAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVL 
                   
               
               
                   
                 VLIVDAVLQLSPLLGAVTVVSMTLLLLAFVLWLSSPGGLGTL 
                   
               
               
                   
                 GAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICS 
                   
               
               
                   
                 SCSSCPLSKILLARLFLYALALLLLASALIAGGSILQTNFKSLSS 
                   
               
               
                   
                 TEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTYGPVFMCLGG 
                   
               
               
                   
                 LLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGVIRC 
                   
               
               
                   
                 CRYCCYYCLTLESEERPPTPYRNTV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV LMP2 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 181 consists of from 5′ end to 3′ end: 
                 181 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 76, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGGGUCCCUAGAAAUGGUGCCAAUGGGCGCGGGUCCU 
                  76 
               
               
                 Construct 
                 CCUAGCCCCGGCGGGGAUCCGGAUGGGUACGAUGGCGGA 
                   
               
               
                 (excluding  
                 AACAACUCCCAAUAUCCAUCUGCUUCUGGCUCUUCUGGG 
                   
               
               
                 the stop 
                 AACACGCCCACCCCACCGAACGAUGAGGAACGUGAAUCU 
                   
               
               
                 codon) 
                 AAUGAAGAGCCACCACCGCCUUAUGAGGACCCAUAUUGG 
                   
               
               
                   
                 GGCAAUGGCGACCGUCACUCGGACUAUCAACCACUAGGA 
                   
               
               
                   
                 ACCCAAGAUCAAAGUCUGUACUUGGGAUUGCAACACGAC 
                   
               
               
                   
                 GGGAAUGACGGGCUCCCUCCACCUCCCUACUCUCCACGGG 
                   
               
               
                   
                 AUGACUCAUCUCAACACAUAUACGAAGAAGCGGGCAGAG 
                   
               
               
                   
                 GAAGUAUGAAUCCAGUAUGCCUGCCUGUAAUUGUUGCGC 
                   
               
               
                   
                 CCUACCUCUUUUGGCUGGCGGCUAUUGCCGCCUCGUGUU 
                   
               
               
                   
                 UCACGGCCUCAGUUAGUACCGUUGUGACCGCCACCGGCU 
                   
               
               
                   
                 UGGCCCUCUCACUUCUACUCUUGGCAGCAGUGGCCAGCUC 
                   
               
               
                   
                 AUAUGCCGCUGCACAAAGGAAACUGCUGACACCGGUGAC 
                   
               
               
                   
                 AGUGCUUACUGCGGUUGUCACUUUCUUUGCAAUUUGCCU 
                   
               
               
                   
                 AACAUGGAGGAUUGAGGACCCACCUUUUAAUUCUCUUCU 
                   
               
               
                   
                 GUUUGCAUUGCUGGCCGCAGCUGGCGGACUACAAGGCAU 
                   
               
               
                   
                 UUACGUUCUGGUGAUGCUUGUGCUCCUGAUACUAGCGUA 
                   
               
               
                   
                 CAGAAGGAGAUGGCGCCGUUUGACUGUUUGUGGCGGCAU 
                   
               
               
                   
                 CAUGUUCUUGGCAUGUGUACUUGUCCUCAUCGUCGACGC 
                   
               
               
                   
                 UGUUUUGCAGCUGAGUCCCCUCCUUGGAGCUGUAACUGU 
                   
               
               
                   
                 GGUUUCCAUGACGCUGCUGCUACUGGCUUUCGUCCUCUG 
                   
               
               
                   
                 GCUCUCUUCGCCAGGUGGCCUAGGUACUCUUGGUGCAGC 
                   
               
               
                   
                 CCUUUUAACAUUGGCAGCAGCUCUGGCACUGCUAGCGUC 
                   
               
               
                   
                 ACUGAUUUUGGGCACACUUAACUUGACUACAAUGUUCCU 
                   
               
               
                   
                 UCUCAUGCUCCUAUGGACACUUGUGGUUCUCCUGAUUUG 
                   
               
               
                   
                 CUCUUCGUGCUCUUCAUGUCCACUGAGCAAGAUCCUUCU 
                   
               
               
                   
                 GGCACGACUGUUCCUAUAUGCUCUCGCACUCUUGUUGCU 
                   
               
               
                   
                 AGCCUCCGCGCUAAUCGCUGGUGGCAGUAUUUUGCAAAC 
                   
               
               
                   
                 AAACUUCAAGAGUUUAAGCAGCACUGAAUUUAUACCCAA 
                   
               
               
                   
                 UUUGUUCUGCAUGUUAUUACUGAUUGUCGCUGGCAUACU 
                   
               
               
                   
                 CUUCAUUCUUGCUAUCCUGACCGAAUGGGGCAGUGGAAA 
                   
               
               
                   
                 UAGAACAUACGGUCCAGUCUUUAUGUGCCUCGGUGGCCU 
                   
               
               
                   
                 GCUCACCAUGGUAGCCGGCGCUGUGUGGCUGACGGUGAU 
                   
               
               
                   
                 GUCUAACACGCUUUUGUCUGCCUGGAUUCUUACAGCAGG 
                   
               
               
                   
                 AUUCCUGAUUUUCCUCAUUGGCUUUGCCCUCUUUGGGGU 
                   
               
               
                   
                 CAUUAGAUGCUGCCGCUACUGCUGCUACUACUGCCUUAC 
                   
               
               
                   
                 ACUGGAAAGUGAGGAGCGCCCACCGACCCCAUAUCGCAA 
                   
               
               
                   
                 CACUGUA 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNT 
                  22 
               
               
                 amino acid 
                 PTPPNDEERESNEEPPPPYEDPYWGNGDRHSDYQPLGTQDQSL 
                   
               
               
                 sequence 
                 YLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLP 
                   
               
               
                   
                 VIVAPYLFWLAAIAASCFTASVSTVVTATGLALSLLLLAAVAS 
                   
               
               
                   
                 SYAAAQRKLLTPVTVLTAVVTFFAICLTWRIEDPPFNSLLFALL 
                   
               
               
                   
                 AAAGGLQGIYVLVMLVLLILAYRRRWRRLTVCGGIMFLACVL 
                   
               
               
                   
                 VLIVDAVLQLSPLLGAVTVVSMTLLLLAFVLWLSSPGGLGTL 
                   
               
               
                   
                 GAALLTLAAALALLASLILGTLNLTTMFLLMLLWTLVVLLICS 
                   
               
               
                   
                 SCSSCPLSKILLARLFLYALALLLLASALIAGGSILQTNFKSLSS 
                   
               
               
                   
                 TEFIPNLFCMLLLIVAGILFILAILTEWGSGNRTYGPVFMCLGG 
                   
               
               
                   
                 LLTMVAGAVWLTVMSNTLLSAWILTAGFLIFLIGFALFGVIRC 
                   
               
               
                   
                 CRYCCYYCLTLESEERPPTPYRNTV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350_003 (modified) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 182 consists of from 5′ end to 3′ end: 
                 182 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 77, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAAGCCGCUCUUCUGGUCUGCCAGUAUACAAUCCAG 
                  77 
               
               
                 Construct 
                 UCGCUGAUUCAUCUCACCGGCGAGGAUCCAGGUUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAGAUUCCAGAAUUCCCUUUCUAUCCAACUUGU 
                   
               
               
                 the stop 
                 AACGUGUGUACUGCCGACGUUAACGUUACUAUUAACUUU 
                   
               
               
                 codon) 
                 GACGUGGGCGGAAAGAAGCACCAGCUGGAUCUUGAUUUC 
                   
               
               
                   
                 GGCCAGCUAACACCUCAUACCAAGGCCGUCUACCAACCAC 
                   
               
               
                   
                 GCGGGGCAUUUGGCGGUUCUGAGAAUGCUACAAACCUGU 
                   
               
               
                   
                 UCCUUUUGGAACUCCUCGGAGCAGGGGAACUGGCACUUA 
                   
               
               
                   
                 CAAUGAGGAGUAAGAAGCUGCCCAUCAACGUCACCACGG 
                   
               
               
                   
                 GUGAAGAACAGCAGGUGUCCCUGGAGUCCGUUGAUGUGU 
                   
               
               
                   
                 AUUUCCAGGACGUUUUCGGGACAAUGUGGUGUCAUCAUG 
                   
               
               
                   
                 CCGAAAUGCAGAAUCCGGUGUAUCUCAUUCCUGAGACCG 
                   
               
               
                   
                 UGCCCUAUAUUAAAUGGGAUAACUGUAAUUCGACAAACA 
                   
               
               
                   
                 UAACUGCCGUAGUGCGUGCCCAGGGUCUGGAUGUAACUU 
                   
               
               
                   
                 UGCCUCUCUCCCUUCCCACUUCUGCCCAGGACUCCAAUUU 
                   
               
               
                   
                 CUCAGUGAAGACCGAAAUGCUUGGGAACGAGAUCGACAU 
                   
               
               
                   
                 CGAGUGUAUCAUGGAGGAUGGGGAGAUCAGUCAGGUGUU 
                   
               
               
                   
                 GCCUGGUGACAAUAAGUUUAAUAUAACAUGUUCCGGCUA 
                   
               
               
                   
                 UGAAUCACAUGUUCCAAGCGGAGGUAUUCUCACAAGUAC 
                   
               
               
                   
                 AUCUCCCGUAGCCACGCCGAUUCCGGGAACCGGAUACGCC 
                   
               
               
                   
                 UAUUCACUUCGGCUGACUCCUCGGCCAGUGUCACGAUUC 
                   
               
               
                   
                 CUGGGCAAUAACUCAAUUCUGUACGUCUUUUACAGCGGC 
                   
               
               
                   
                 AACGGCCCAAAGGCCAGUGGAGGCGAUUACUGUAUCCAG 
                   
               
               
                   
                 UCUAACAUCGUAUUUUCAGAUGAGAUUCCAGCAAGCCAA 
                   
               
               
                   
                 GACAUGCCAACUAAUACUACAGACAUUACAUACGUCGGU 
                   
               
               
                   
                 GAUAACGCCACUUAUUCAGUCCCUAUGGUCACAUCCGAG 
                   
               
               
                   
                 GACGCGAACUCACCCAAUGUUACCGUUACCGCAUUCUGG 
                   
               
               
                   
                 GCAUGGCCCAAUAAUACGGAAACAGACUUCAAAUGUAAG 
                   
               
               
                   
                 UGGACCCUGACCAGUGGCACCCCUUCUGGCUGCGAGAAU 
                   
               
               
                   
                 AUAAGCGGGGCAUUUGCUAGCAACAGAACAUUCGAUAUU 
                   
               
               
                   
                 ACUGUGUCUGGCCUGGGGACCGCGCCCAAGACACUCAUU 
                   
               
               
                   
                 AUAACGCGAACAGCUACGAACGCUACCACAACCACACAU 
                   
               
               
                   
                 AAAGUGAUUUUCAGCAAGGCUCCAGAAUCAACUACAACC 
                   
               
               
                   
                 AGUCCGACCCUUAAUACCACAGGCUUCGCAGACCCGAACA 
                   
               
               
                   
                 CAACGACGGGACUGCCCUCCUCAACGCAUGUGCCGACUAA 
                   
               
               
                   
                 UCUGACUGCUCCCGCAAGUACCGGCCCAACAGUCUCCACA 
                   
               
               
                   
                 GCAGACGUCACCUCUCCGACUCCCGCCGGCACUACAUCUG 
                   
               
               
                   
                 GAGCCUCACCGGUGACUCCUAGUCCUUCGCCCUGGGACAA 
                   
               
               
                   
                 UGGGACCGAGUCUAAAGCCCCGGACAUGACGAGUUCUAC 
                   
               
               
                   
                 CUCACCAGUUACAACCCCAACACCUAAUGCCACAAGCCCU 
                   
               
               
                   
                 ACGCCAGCCGUGACCACCCCUACGCCCAAUGCCACCAGCC 
                   
               
               
                   
                 CAACACCCGCUGUUACAACGCCAACGCCCAACGCUACUUC 
                   
               
               
                   
                 UCCAACGCUAGGAAAGACAUCACCCACCUCAGCUGUAACC 
                   
               
               
                   
                 ACGCCCACACCAAACGCUACCGGGCCAACCGUUGGCGAAA 
                   
               
               
                   
                 CCUCGCCCCAGGCCAAUGCGACAAACCACACCCUUGGCGG 
                   
               
               
                   
                 GACUUCUCCCACUCCUGUGGUGACUUCACAACCGAAGAA 
                   
               
               
                   
                 CGCGACGUCGGCCGUUACGACCGGGCAGCACAAUAUCACC 
                   
               
               
                   
                 UCGUCCUCCACGUCGUCCAUGUCCUUGCGGCCUUCCAGUA 
                   
               
               
                   
                 ACCCAGAGACACUGUCUCCUAGUACCAGUGACAACAGCA 
                   
               
               
                   
                 CCUCUCACAUGCCAUUACUGACUUCCGCCCACCCAACCGG 
                   
               
               
                   
                 CGGAGAGAACAUCACUCAGGUGACGCCCGCAUCUAUUUC 
                   
               
               
                   
                 UACCCACCACGUGAGCACUAGCAGCCCGGCACCAAGACCA 
                   
               
               
                   
                 GGGACCACCUCCCAGGCUAGCGGCCCGGGCAACUCCUCCA 
                   
               
               
                   
                 CUAGCACGAAACCAGGAGAGGUGAACGUGACCAAGGGCA 
                   
               
               
                   
                 CACCGCCACAGAAUGCAACUUCCCCACAGGCGCCCUCCGG 
                   
               
               
                   
                 CCAGAAGACUGCUGUACCUACGGUCACUAGCACUGGCGG 
                   
               
               
                   
                 AAAGGCGAAUUCAACCACCGGUGGUAAGCAUACCACCGG 
                   
               
               
                   
                 ACACGGCGCUCGGACUAGCACAGAGCCAACCACUGAUUA 
                   
               
               
                   
                 CGGAGGUGACUCAACGACCCCGAGGCCCAGGUACAACGC 
                   
               
               
                   
                 AACCACCUAUCUCCCACCAUCCACAUCUUCCAAGCUCAGG 
                   
               
               
                   
                 CCUCGUUGGACUUUCACCUCCCCGCCUGUGACUACAGCGC 
                   
               
               
                   
                 AGGCCACAGUGCCAGUGCCUCCAACGUCACAGCCGCGGUU 
                   
               
               
                   
                 UAGCAAUCUCUCCAUGCUGGUCCUGCAGUGGGCAUCUCU 
                   
               
               
                   
                 GGCAGUCCUGACACUGCUUCUUUUGCUCGUGAUGGCCGA 
                   
               
               
                   
                 UUGUGCGUUUAGACGCAACCUUUCAACAUCCCACACAUA 
                   
               
               
                   
                 UACAACUCCACCCUAUGAUGACGCAGAGACUUAUGUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  78 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gH (BXLF2)_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 183 consists of from 5′ end to 3′ end: 
                 183 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 79, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGCAGUUGUUGUGCGUGUUCUGCCUCGUGUUACUCUGG 
                  79 
               
               
                 Construct 
                 GAGGUGGGCGCCGCCAGCCUUAGCGAGGUGAAGCUCCAC 
                   
               
               
                 (excluding 
                 UUGGACAUCGAGGGCCACGCCAGCCACUACACCAUCCCCU 
                   
               
               
                 the stop 
                 GGACCGAGCUCAUGGCCAAGGUGCCCGGCCUUAGCCCCGA 
                   
               
               
                 codon) 
                 GGCCCUGUGGCGGGAGGCCAACGUGACCGAGGACCUGGC 
                   
               
               
                   
                 CAGCAUGCUGAACCGGUACAAGCUGAUCUACAAGACCAG 
                   
               
               
                   
                 CGGCACCCUGGGCAUCGCCCUGGCCGAGCCCGUGGACAUC 
                   
               
               
                   
                 CCCGCCGUUAGCGAAGGCAGCAUGCAGGUGGACGCCAGC 
                   
               
               
                   
                 AAGGUGCACCCCGGCGUGAUCAGCGGCCUGAACAGCCCCG 
                   
               
               
                   
                 CCUGUAUGUUGAGCGCCCCACUGGAGAAGCAGCUGUUCU 
                   
               
               
                   
                 ACUACAUCGGCACCAUGCUGCCCAACACCCGGCCCCACAG 
                   
               
               
                   
                 CUACGUGUUCUACCAGCUGCGGUGCCACCUGAGCUACGU 
                   
               
               
                   
                 UGCCCUGAGCAUCAACGGCGACAAGUUCCAGUACACCGG 
                   
               
               
                   
                 CGCCAUGACCAGCAAGUUCCUGAUGGGCACCUACAAGCG 
                   
               
               
                   
                 GGUCACCGAGAAGGGCGACGAGCACGUGCUGUCACUGGU 
                   
               
               
                   
                 GUUCGGCAAGACCAAGGACCUGCCCGACCUGCGGGGCCCC 
                   
               
               
                   
                 UUCAGCUACCCUAGUUUGACCAGCGCCCAGAGCGGCGAC 
                   
               
               
                   
                 UACAGCUUGGUGAUCGUGACCACCUUCGUGCACUACGCC 
                   
               
               
                   
                 AACUUCCACAACUACUUCGUGCCCAACCUGAAGGACAUG 
                   
               
               
                   
                 UUCAGCCGGGCCGUGACCAUGACUGCCGCUUCUUACGCCC 
                   
               
               
                   
                 GGUACGUGCUGCAGAAGCUGGUCCUGCUGGAGAUGAAGG 
                   
               
               
                   
                 GCGGCUGCCGGGAGCCCGAGCUGGACACCGAAACACUGA 
                   
               
               
                   
                 CCACCAUGUUCGAGGUGAGCGUGGCCUUCUUCAAGGUGG 
                   
               
               
                   
                 GUCACGCGGUGGGCGAAACCGGCAACGGCUGCGUGGACU 
                   
               
               
                   
                 UACGCUGGCUGGCCAAGAGCUUCUUCGAGCUGACCGUGC 
                   
               
               
                   
                 UGAAGGAUAUCAUCGGCAUCUGCUACGGCGCCACCGUGA 
                   
               
               
                   
                 AGGGCAUGCAGAGCUACGGCCUGGAGCGGCUGGCCGCCA 
                   
               
               
                   
                 UGCUUAUGGCAACAGUGAAGAUGGAGGAGCUGGGACACC 
                   
               
               
                   
                 UGACAACAGAGAAGCAGGAGUACGCCCUGAGACUGGCCA 
                   
               
               
                   
                 CAGUGGGCUACCCAAAGGCCGGCGUGUACAGUGGACUGA 
                   
               
               
                   
                 UCGGCGGCGCAACCAGCGUGCUGCUAUCCGCUUACAACCG 
                   
               
               
                   
                 GCACCCGCUGUUCCAGCCCCUGCACACCGUGAUGCGGGAA 
                   
               
               
                   
                 ACCCUGUUCAUCGGAAGCCACGUCGUGCUGCGGGAGCUG 
                   
               
               
                   
                 AGGCUGAACGUAACCACCCAGGGCCCUAAUCUGGCCCUG 
                   
               
               
                   
                 UAUCAGCUCCUCAGUACCGCCCUGUGCAGCGCCCUUGAGA 
                   
               
               
                   
                 UCGGCGAGGUGCUCAGAGGCCUGGCCCUCGGUACCGAGA 
                   
               
               
                   
                 GCGGCCUCUUCAGCCCAUGCUACUUAAGCCUGCGGUUCG 
                   
               
               
                   
                 ACCUGACCCGGGACAAGUUGCUGAGCAUGGCCCCGCAGG 
                   
               
               
                   
                 AGGCCACACUGGACCAGGCAGCUGUAUCCAACGCCGUGG 
                   
               
               
                   
                 ACGGCUUCCUGGGCAGACUGUCCCUGGAACGGGAGGACC 
                   
               
               
                   
                 GGGACGCCUGGCACCUGCCUGCCUACAAGUGUGUGGAUC 
                   
               
               
                   
                 GGCUGGACAAGGUGCUGAUGAUCAUCCCUCUGAUUAAUG 
                   
               
               
                   
                 UCACCUUCAUCAUCAGCAGCGACCGGGAGGUGCGGGGAU 
                   
               
               
                   
                 CCGCCCUCUACGAGGCCAGCACCACCUAUCUGAGCAGCAG 
                   
               
               
                   
                 CCUGUUCCUGUCUCCUGUGAUCAUGAACAAGUGCAGCCA 
                   
               
               
                   
                 GGGCGCCGUGGCCGGCGAGCCCCGGCAGAUCCCCAAGAUC 
                   
               
               
                   
                 CAGAACUUCACCCGGACCCAGAAGUCUUGCAUCUUCUGC 
                   
               
               
                   
                 GGCUUCGCCCUUUUGUCCUACGACGAGAAGGAGGGCUUG 
                   
               
               
                   
                 GAGACUACAACCUACAUCACCAGCCAGGAGGUGCAGAAC 
                   
               
               
                   
                 AGCAUCCUGUCAUCUAAUUACUUCGACUUCGACAACCUG 
                   
               
               
                   
                 CACGUUCAUUACCUGCUCCUCACCACCAACGGUACCGUCA 
                   
               
               
                   
                 UGGAAAUCGCCGGACUGUACGAGGAGCGGGCCCAUGUUG 
                   
               
               
                   
                 UGCUGGCCAUCAUCCUGUACUUCAUCGCUUUCGCACUUG 
                   
               
               
                   
                 GCAUCUUCCUGGUGCACAAGAUCGUGAUGUUCUUCCUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MQLLCVFCLVLLWEVGAASLSEVKLHLDIEGHASHYTIPWTE 
                  38 
               
               
                 amino acid 
                 LMAKVPGLSPEALWREANVTEDLASMLNRYKLIYKTSGTLGI 
                   
               
               
                 sequence 
                 ALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSPACMLSAPLE 
                   
               
               
                   
                 KQLFYYIGTMLPNTRPHSYVFYQLRCHLSYVALSINGDKFQYT 
                   
               
               
                   
                 GAMTSKFLMGTYKRVTEKGDEHVLSLVFGKTKDLPDLRGPFS 
                   
               
               
                   
                 YPSLTSAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMFSRAV 
                   
               
               
                   
                 TMTAASYARYVLQKLVLLEMKGGCREPELDTETLTTMFEVS 
                   
               
               
                   
                 VAFFKVGHAVGETGNGCVDLRWLAKSFFELTVLKDIIGICYG 
                   
               
               
                   
                 ATVKGMQSYGLERLAAMLMATVKMEELGHLTTEKQEYALR 
                   
               
               
                   
                 LATVGYPKAGVYSGLIGGATSVLLSAYNRHPLFQPLHTVMRE 
                   
               
               
                   
                 TLFIGSHVVLRELRLNVTTQGPNLALYQLLSTALCSALEIGEVL 
                   
               
               
                   
                 RGLALGTESGLFSPCYLSLRFDLTRDKLLSMAPQEATLDQAA 
                   
               
               
                   
                 VSNAVDGFLGRLSLEREDRDAWHLPAYKCVDRLDKVLMIIPL 
                   
               
               
                   
                 INVTFIISSDREVRGSALYEASTTYLSSSLFLSPVIMNKCSQGAV 
                   
               
               
                   
                 AGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKEGLETTTYITSQ 
                   
               
               
                   
                 EVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEIAGLYEERAH 
                   
               
               
                   
                 VVLAIILYFIAFALGIFLVHKIVMFFL 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gL (B95-8) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 184 consists of from 5′ end to 3′ end: 
                 184 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 80, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGAGCCGUGGGCGUGUUCCUGGCCAUCUGCCUGGUG 
                  80 
               
               
                 Construct 
                 ACCAUCUUCGUGCUGCCCACCUGGGGCAACUGGGCCUACC 
                   
               
               
                 (excluding 
                 CCUGCUGCCACGUGACCCAGCUGAGAGCCCAGCACCUGCU 
                   
               
               
                 the stop 
                 GGCCCUGGAGAACAUCAGCGACAUCUACCUGGUGAGCAA 
                   
               
               
                 codon) 
                 CCAGACCUGCGACGGCUUCAGCCUGGCCAGCCUGAACAGC 
                   
               
               
                   
                 CCCAAGAACGGCAGCAACCAGCUGGUGAUCAGCAGAUGC 
                   
               
               
                   
                 GCCAACGGCCUGAACGUGGUGAGCUUCUUCAUCAGCAUC 
                   
               
               
                   
                 CUGAAGAGAAGCAGCAGCGCCCUGACCGGCCACCUGAGA 
                   
               
               
                   
                 GAGCUGCUGACCACCCUGGAGACCCUGUACGGCAGCUUC 
                   
               
               
                   
                 AGCGUGGAGGACCUGUUCGGCGCCAACCUGAACAGAUAC 
                   
               
               
                   
                 GCCUGGCACAGAGGCGGC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MRAVGVFLAICLVTIFVLPTWGNWAYPCCHVTQLRAQHLLA 
                  36 
               
               
                 amino acid 
                 LENISDIYLVSNQTCDGFSLASLNSPKNGSNQLVISRCANGLNV 
                   
               
               
                 sequence 
                 VSFFISILKRSSSALTGHLRELLTTLETLYGSFSVEDLFGANLNR 
                   
               
               
                   
                 YAWHRGG 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350_001 (modified) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 185 consists of from 5′ end to 3′ end: 
                 185 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 81, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  81 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  28 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 LMP1 del 1-44_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 186 consists of from 5′ end to 3′ end: 
                 186 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 82, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGUGACUGGACAGGAGGAGCCCUCCUUGUCCUCUAU 
                  82 
               
               
                 Construct 
                 UCCUUUGCUCUCAUGCUUAUAAUUAUCAUCCUGAUAAUC 
                   
               
               
                 (excluding 
                 UUUAUCUUCAGACGGGACCUUCUCUGUCCACUUGGAGCC 
                   
               
               
                 the stop 
                 CUUUGUAUACUCCUAUUGAUGAUCACCCUCCUCCUCAUC 
                   
               
               
                 codon) 
                 GCUCUCUGGAAUUUGCACGGACAGGCAUUGUUCCUUGGA 
                   
               
               
                   
                 AUUGUGCUGUUCAUCUUCGGGUGCUUACUUGUCUUAGGU 
                   
               
               
                   
                 AUCUGGAUCUACUUAUUGGAGAUGCUCUGGCGACUUGGU 
                   
               
               
                   
                 GCCACCAUCUGGCAGCUUUUGGCCUUCUUCCUAGCGUUC 
                   
               
               
                   
                 UUCCUCGACCUCAUUCUACUCAUUAUUGCUCUCUAUCUA 
                   
               
               
                   
                 CAACAGAACUGGUGGACUCUAUUGGUUGAUCUCCUUUGG 
                   
               
               
                   
                 CUCCUCCUGUUUCUGGCGAUUUUAAUCUGGAUGUAUUAC 
                   
               
               
                   
                 CAUGGACAACGACACAGUGAUGAACACCACCACGAUGAC 
                   
               
               
                   
                 UCCCUCCCGCACCCUCAACAAGCUACCGAUGAUUCUGGCC 
                   
               
               
                   
                 AUGAAUCUGACUCUAACUCCAACGAGGGCAGACACCACC 
                   
               
               
                   
                 UGCUCGUGAGUGGAGCCGGCGACGGACCACCACUCUGCU 
                   
               
               
                   
                 CUCAGAACCUAGGCGCACCUGGAGGUGGUCCUGACAAUG 
                   
               
               
                   
                 GCCCACAGGACCCUGACAACACGGAUGAUAACGGUCCAC 
                   
               
               
                   
                 AAGACCCAGAUAACACUGACGACAAUGGACCGCAUGACC 
                   
               
               
                   
                 CGCUGCCUCAGGAUCCUGAUAACACAGACGACAACGGCCC 
                   
               
               
                   
                 ACAAGAUCCGGACAAUACCGACGAUAACGGACCGCACGA 
                   
               
               
                   
                 CCCGUUACCUCAUAGCCCUAGCGACUCUGCUGGAAAUGA 
                   
               
               
                   
                 UGGAGGCCCUCCACAAUUGACGGAAGAGGUUGAGAACAA 
                   
               
               
                   
                 AGGAGGUGACCAGGGCCCGCCUUUGAUGACAGACGGAGG 
                   
               
               
                   
                 CGGCGGUCAUAGUCAUGAUUCCGGCCAUGGCGGAGGAGA 
                   
               
               
                   
                 UCCACACCUUCCUACGCUGCUGUUGGGUUCUUCUGGUUC 
                   
               
               
                   
                 CGGUGGAGAUGAUGACGACCCGCACGGCCCAGUUCAGCU 
                   
               
               
                   
                 AAGCUACUAUGAC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MSDWTGGALLVLYSFALMLIIIILIIFIFRRDLLCPLGALCILLLM 
                  83 
               
               
                 amino acid 
                 ITLLLIALWNLHGQALFLGIVLFIFGCLLVLGIWIYLLEMLWRL 
                   
               
               
                 sequence 
                 GATIWQLLAFFLAFFLDLILLIIALYLQQNWWTLLVDLLWLLL 
                   
               
               
                   
                 FLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSN 
                   
               
               
                   
                 SNEGRHHLLVSGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTD 
                   
               
               
                   
                 DNGPQDPDNTDDNGPHDPLPQDPDNTDDNGPQDPDNTDDNG 
                   
               
               
                   
                 PHDPLPHSPSDSAGNDGGPPQLTEEVENKGGDQGPPLMTDGG 
                   
               
               
                   
                 GGHSHDSGHGGGDPHLPTLLLGSSGSGGDDDDPHGPVQLSYY 
                   
               
               
                   
                 D 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gH (BXLF2)_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 187 consists of from 5′ end to 3′ end: 
                 187 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 84, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGCAGUUGUUGUGCGUGUUCUGCCUCGUGUUACUCUGG 
                  84 
               
               
                 Construct 
                 GAGGUGGGCGCCGCCAGCCUUAGCGAGGUGAAGCUCCAC 
                   
               
               
                 (excluding 
                 UUGGACAUCGAGGGCCACGCCAGCCACUACACCAUCCCCU 
                   
               
               
                 the stop 
                 GGACCGAGCUCAUGGCCAAGGUGCCCGGCCUUAGCCCCGA 
                   
               
               
                 codon) 
                 GGCCCUGUGGCGGGAGGCCAACGUGACCGAGGACCUGGC 
                   
               
               
                   
                 CAGCAUGCUGAACCGGUACAAGCUGAUCUACAAGACCAG 
                   
               
               
                   
                 CGGCACCCUGGGCAUCGCCCUGGCCGAGCCCGUGGACAUC 
                   
               
               
                   
                 CCCGCCGUUAGCGAAGGCAGCAUGCAGGUGGACGCCAGC 
                   
               
               
                   
                 AAGGUGCACCCCGGCGUGAUCAGCGGCCUGAACAGCCCCG 
                   
               
               
                   
                 CCUGUAUGUUGAGCGCCCCACUGGAGAAGCAGCUGUUCU 
                   
               
               
                   
                 ACUACAUCGGCACCAUGCUGCCCAACACCCGGCCCCACAG 
                   
               
               
                   
                 CUACGUGUUCUACCAGCUGCGGUGCCACCUGAGCUACGU 
                   
               
               
                   
                 UGCCCUGAGCAUCAACGGCGACAAGUUCCAGUACACCGG 
                   
               
               
                   
                 CGCCAUGACCAGCAAGUUCCUGAUGGGCACCUACAAGCG 
                   
               
               
                   
                 GGUCACCGAGAAGGGCGACGAGCACGUGCUGUCACUGGU 
                   
               
               
                   
                 GUUCGGCAAGACCAAGGACCUGCCCGACCUGCGGGGCCCC 
                   
               
               
                   
                 UUCAGCUACCCUAGUUUGACCAGCGCCCAGAGCGGCGAC 
                   
               
               
                   
                 UACAGCUUGGUGAUCGUGACCACCUUCGUGCACUACGCC 
                   
               
               
                   
                 AACUUCCACAACUACUUCGUGCCCAACCUGAAGGACAUG 
                   
               
               
                   
                 UUCAGCCGGGCCGUGACCAUGACUGCCGCUUCUUACGCCC 
                   
               
               
                   
                 GGUACGUGCUGCAGAAGCUGGUCCUGCUGGAGAUGAAGG 
                   
               
               
                   
                 GCGGCUGCCGGGAGCCCGAGCUGGACACCGAAACACUGA 
                   
               
               
                   
                 CCACCAUGUUCGAGGUGAGCGUGGCCUUCUUCAAGGUGG 
                   
               
               
                   
                 GUCACGCGGUGGGCGAAACCGGCAACGGCUGCGUGGACU 
                   
               
               
                   
                 UACGCUGGCUGGCCAAGAGCUUCUUCGAGCUGACCGUGC 
                   
               
               
                   
                 UGAAGGAUAUCAUCGGCAUCUGCUACGGCGCCACCGUGA 
                   
               
               
                   
                 AGGGCAUGCAGAGCUACGGCCUGGAGCGGCUGGCCGCCA 
                   
               
               
                   
                 UGCUUAUGGCAACAGUGAAGAUGGAGGAGCUGGGACACC 
                   
               
               
                   
                 UGACAACAGAGAAGCAGGAGUACGCCCUGAGACUGGCCA 
                   
               
               
                   
                 CAGUGGGCUACCCAAAGGCCGGCGUGUACAGUGGACUGA 
                   
               
               
                   
                 UCGGCGGCGCAACCAGCGUGCUGCUAUCCGCUUACAACCG 
                   
               
               
                   
                 GCACCCGCUGUUCCAGCCCCUGCACACCGUGAUGCGGGAA 
                   
               
               
                   
                 ACCCUGUUCAUCGGAAGCCACGUCGUGCUGCGGGAGCUG 
                   
               
               
                   
                 AGGCUGAACGUAACCACCCAGGGCCCUAAUCUGGCCCUG 
                   
               
               
                   
                 UAUCAGCUCCUCAGUACCGCCCUGUGCAGCGCCCUUGAGA 
                   
               
               
                   
                 UCGGCGAGGUGCUCAGAGGCCUGGCCCUCGGUACCGAGA 
                   
               
               
                   
                 GCGGCCUCUUCAGCCCAUGCUACUUAAGCCUGCGGUUCG 
                   
               
               
                   
                 ACCUGACCCGGGACAAGUUGCUGAGCAUGGCCCCGCAGG 
                   
               
               
                   
                 AGGCCACACUGGACCAGGCAGCUGUAUCCAACGCCGUGG 
                   
               
               
                   
                 ACGGCUUCCUGGGCAGACUGUCCCUGGAACGGGAGGACC 
                   
               
               
                   
                 GGGACGCCUGGCACCUGCCUGCCUACAAGUGUGUGGAUC 
                   
               
               
                   
                 GGCUGGACAAGGUGCUGAUGAUCAUCCCUCUGAUUAAUG 
                   
               
               
                   
                 UCACCUUCAUCAUCAGCAGCGACCGGGAGGUGCGGGGAU 
                   
               
               
                   
                 CCGCCCUCUACGAGGCCAGCACCACCUAUCUGAGCAGCAG 
                   
               
               
                   
                 CCUGUUCCUGUCUCCUGUGAUCAUGAACAAGUGCAGCCA 
                   
               
               
                   
                 GGGCGCCGUGGCCGGCGAGCCCCGGCAGAUCCCCAAGAUC 
                   
               
               
                   
                 CAGAACUUCACCCGGACCCAGAAGUCUUGCAUCUUCUGC 
                   
               
               
                   
                 GGCUUCGCCCUUUUGUCCUACGACGAGAAGGAGGGCUUG 
                   
               
               
                   
                 GAGACUACAACCUACAUCACCAGCCAGGAGGUGCAGAAC 
                   
               
               
                   
                 AGCAUCCUGUCAUCUAAUUACUUCGACUUCGACAACCUG 
                   
               
               
                   
                 CACGUUCAUUACCUGCUCCUCACCACCAACGGUACCGUCA 
                   
               
               
                   
                 UGGAAAUCGCCGGACUGUACGAGGAGCGGGCCCAUGUUG 
                   
               
               
                   
                 UGCUGGCCAUCAUCCUGUACUUCAUCGCUUUCGCACUUG 
                   
               
               
                   
                 GCAUCUUCCUGGUGCACAAGAUCGUGAUGUUCUUCCUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MQLLCVFCLVLLWEVGAASLSEVKLHLDIEGHASHYTIPWTE 
                  38 
               
               
                 amino acid 
                 LMAKVPGLSPEALWREANVTEDLASMLNRYKLIYKTSGTLGI 
                   
               
               
                 sequence 
                 ALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSPACMLSAPLE 
                   
               
               
                   
                 KQLFYYIGTMLPNTRPHSYVFYQLRCHLSYVALSINGDKFQYT 
                   
               
               
                   
                 GAMTSKFLMGTYKRVTEKGDEHVLSLVFGKTKDLPDLRGPFS 
                   
               
               
                   
                 YPSLTSAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMFSRAV 
                   
               
               
                   
                 TMTAASYARYVLQKLVLLEMKGGCREPELDTETLTTMFEVS 
                   
               
               
                   
                 VAFFKVGHAVGETGNGCVDLRWLAKSFFELTVLKDIIGICYG 
                   
               
               
                   
                 ATVKGMQSYGLERLAAMLMATVKMEELGHLTTEKQEYALR 
                   
               
               
                   
                 LATVGYPKAGVYSGLIGGATSVLLSAYNRHPLFQPLHTVMRE 
                   
               
               
                   
                 TLFIGSHVVLRELRLNVTTQGPNLALYQLLSTALCSALEIGEVL 
                   
               
               
                   
                 RGLALGTESGLFSPCYLSLRFDLTRDKLLSMAPQEATLDQAA 
                   
               
               
                   
                 VSNAVDGFLGRLSLEREDRDAWHLPAYKCVDRLDKVLMIIPL 
                   
               
               
                   
                 INVTFIISSDREVRGSALYEASTTYLSSSLFLSPVIMNKCSQGAV 
                   
               
               
                   
                 AGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKEGLETTTYITSQ 
                   
               
               
                   
                 EVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEIAGLYEERAH 
                   
               
               
                   
                 VVLAIILYFIAFALGIFLVHKIVMFFL 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gL (B95-8) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 188 consists of from 5′ end to 3′ end: 
                 188 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 85, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGAGCCGUGGGCGUGUUCCUGGCCAUCUGCCUGGUG 
                  85 
               
               
                 Construct 
                 ACCAUCUUCGUGCUGCCCACCUGGGGCAACUGGGCCUACC 
                   
               
               
                 (excluding 
                 CCUGCUGCCACGUGACCCAGCUGAGAGCCCAGCACCUGCU 
                   
               
               
                 the stop 
                 GGCCCUGGAGAACAUCAGCGACAUCUACCUGGUGAGCAA 
                   
               
               
                 codon) 
                 CCAGACCUGCGACGGCUUCAGCCUGGCCAGCCUGAACAGC 
                   
               
               
                   
                 CCCAAGAACGGCAGCAACCAGCUGGUGAUCAGCAGAUGC 
                   
               
               
                   
                 GCCAACGGCCUGAACGUGGUGAGCUUCUUCAUCAGCAUC 
                   
               
               
                   
                 CUGAAGAGAAGCAGCAGCGCCCUGACCGGCCACCUGAGA 
                   
               
               
                   
                 GAGCUGCUGACCACCCUGGAGACCCUGUACGGCAGCUUC 
                   
               
               
                   
                 AGCGUGGAGGACCUGUUCGGCGCCAACCUGAACAGAUAC 
                   
               
               
                   
                 GCCUGGCACAGAGGCGGC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MRAVGVFLAICLVTIFVLPTWGNWAYPCCHVTQLRAQHLLA 
                  36 
               
               
                 amino acid 
                 LENISDIYLVSNQTCDGFSLASLNSPKNGSNQLVISRCANGLNV 
                   
               
               
                 sequence 
                 VSFFISILKRSSSALTGHLRELLTTLETLYGSFSVEDLFGANLNR 
                   
               
               
                   
                 YAWHRGG 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp42 (B95-8)_DX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 189 consists of from 5′ end to 3′ end: 
                 189 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 86, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGUGAGCUUCAAGCAGGUGAGAGUGCCCCUGUUCACC 
                  86 
               
               
                 Construct 
                 GCCAUCGCCCUGGUGAUCGUGCUGCUGCUGGCCUACUUCC 
                   
               
               
                 (excluding 
                 UGCCCCCCAGAGUGAGAGGCGGCGGCAGAGUGGCCGCCG 
                   
               
               
                 the stop 
                 CCGCCAUCACCUGGGUGCCCAAGCCCAACGUGGAGGUGU 
                   
               
               
                 codon) 
                 GGCCCGUGGACCCCCCACCCCCCGUGAACUUCAACAAGAC 
                   
               
               
                   
                 CGCCGAGCAGGAGUACGGCGACAAGGAGGUGAAGCUGCC 
                   
               
               
                   
                 CCACUGGACCCCCACCCUGCACACCUUCCAGGUGCCCCAG 
                   
               
               
                   
                 AACUACACCAAGGCCAACUGCACCUACUGCAACACCAGAG 
                   
               
               
                   
                 AGUACACCUUCAGCUACAAGGGCUGCUGCUUCUACUUCA 
                   
               
               
                   
                 CCAAGAAGAAGCACACCUGGAACGGCUGCUUCCAGGCCU 
                   
               
               
                   
                 GCGCCGAGCUGUACCCCUGCACCUACUUCUACGGCCCCAC 
                   
               
               
                   
                 CCCCGACAUCCUGCCCGUGGUGACCAGAAACCUGAACGCC 
                   
               
               
                   
                 AUCGAGAGCCUGUGGGUGGGCGUGUACAGAGUGGGCGAG 
                   
               
               
                   
                 GGCAACUGGACCAGCCUGGACGGCGGCACCUUCAAGGUG 
                   
               
               
                   
                 UACCAGAUCUUCGGCAGCCACUGCACCUACGUGAGCAAG 
                   
               
               
                   
                 UUCAGCACCGUGCCCGUGAGCCACCACGAGUGCAGCUUCC 
                   
               
               
                   
                 UGAAGCCCUGCCUGUGCGUGAGCCAGAGAAGCAACAGC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MVSFKQVRVPLFTAIALVIVLLLAYFLPPRVRGGGRVAAAAIT 
                  34 
               
               
                 amino acid 
                 WVPKPNVEVWPVDPPPPVNFNKTAEQEYGDKEVKLPHWTPT 
                   
               
               
                 sequence 
                 LHTFQVPQNYTKANCTYCNTREYTFSYKGCCFYFTKKKHTW 
                   
               
               
                   
                 NGCFQACAELYPCTYFYGPTPDILPVVTRNLNAIESLWVGVYR 
                   
               
               
                   
                 VGEGNWTSLDGGTFKVYQIFGSHCTYVSKFSTVPVSHHECSFL 
                   
               
               
                   
                 KPCLCVSQRSNS 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 gp350 D123_LS_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 190 consists of from 5′ end to 3′ end: 
                 190 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 87, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGACAGCAAGGGCAGCAGCCAGAAGGGAAGCAGGUUG 
                  87 
               
               
                 Construct 
                 UUAUUGCUUUUAGUGGUGAGCAACUUACUCUUACCCCAG 
                   
               
               
                 (excluding 
                 AUCCAGUCGCUCAUUCAUUUGACAGGCGAGGACCCCGGC 
                   
               
               
                 the stop 
                 UUCUUUAACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCA 
                   
               
               
                 codon) 
                 ACCUGUAACGUUUGCACAGCCGACGUGAACGUGACAAUC 
                   
               
               
                   
                 AAUUUCGACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUG 
                   
               
               
                   
                 GAUUUCGGCCAACUGACGCCACACACCAAAGCGGUGUAC 
                   
               
               
                   
                 CAACCAAGGGGUGCAUUUGGAGGGAGCGAGAAUGCUACA 
                   
               
               
                   
                 AAUCUGUUCUUACUGGAACUGCUUGGUGCAGGAGAGCUG 
                   
               
               
                   
                 GCACUAACCAUGCGAAGCAAGAAGCUCCCCAUCAACGUU 
                   
               
               
                   
                 ACCACCGGGGAAGAACAGCAAGUCAGCCUUGAAUCCGUG 
                   
               
               
                   
                 GAUGUGUACUUUCAGGAUGUUUUCGGCACCAUGUGGUGU 
                   
               
               
                   
                 CACCACGCUGAGAUGCAGAAUCCCGUGUAUCUCAUACCC 
                   
               
               
                   
                 GAGACUGUACCCUACAUCAAGUGGGAUAAUUGCAAUUCA 
                   
               
               
                   
                 ACUAAUAUUACGGCUGUGGUGAGGGCCCAGGGCUUGGAC 
                   
               
               
                   
                 GUGACUCUGCCCUUAUCUCUACCUACUUCUGCCCAAGACU 
                   
               
               
                   
                 CCAAUUUCUCUGUCAAGACCGAGAUGCUCGGGAAUGAGA 
                   
               
               
                   
                 UCGAUAUCGAGUGCAUCAUGGAGGACGGUGAGAUAAGCC 
                   
               
               
                   
                 AGGUUCUGCCCGGCGACAACAAGUUCAAUAUCACUUGUU 
                   
               
               
                   
                 CUGGCUACGAGUCCCAUGUGCCUAGUGGUGGCAUACUCA 
                   
               
               
                   
                 CAAGUACUUCUCCCGUAGCCACGCCCAUUCCCGGAACCGG 
                   
               
               
                   
                 AUACGCCUACAGUCUGCGUCUGACCCCACGGCCUGUGUCC 
                   
               
               
                   
                 AGAUUCCUGGGUAACAAUAGUAUCUUAUACGUGUUUUAU 
                   
               
               
                   
                 AGCGGAAACGGCCCUAAAGCGUCCGGAGGGGACUAUUGU 
                   
               
               
                   
                 AUUCAGAGUAAUAUCGUUUUCUCUGAUGAGAUCCCUGCC 
                   
               
               
                   
                 AGUCAGGACAUGCCGACAAACACAACUGAUAUUACCUAC 
                   
               
               
                   
                 GUGGGCGACAAUGCCACGUAUUCAGUGCCCAUGGUCACG 
                   
               
               
                   
                 AGCGAGGACGCCAAUUCACCAAAUGUUACUGUAACAGCU 
                   
               
               
                   
                 UUCUGGGCCUGGCCAAAUAACACUGAGACUGACUUCAAA 
                   
               
               
                   
                 UGUAAGUGGACUUUGACCUCUGGAACUCCGUCGGGUUGC 
                   
               
               
                   
                 GAGAAUAUCAGCGGGGCCUUUGCUUCCAACAGGACUUUC 
                   
               
               
                   
                 GACAUCACUGUCUCAGGGCUGGGGACAGCACCGAAGACA 
                   
               
               
                   
                 UUAAUCAUAACACGGACCGCCACCAACGCCACGACUACAA 
                   
               
               
                   
                 CCCAUAAGGUGAUCUUUUCCAAGGCACCUGGCAGCGGCA 
                   
               
               
                   
                 GCGCAGGCUCUGCAAGCGGCGGCGCCUCCAGCGGCUCCGG 
                   
               
               
                   
                 AGGUGCCAGCAUGCAGAUCUACGAGGGCAAGCUGACCGC 
                   
               
               
                   
                 CGAGGGCCUGAGGUUCGGCAUCGUGGCCAGCAGGUUCAA 
                   
               
               
                   
                 CCACGCCCUGGUGGACAGGCUGGUGGAAGGCGCCAUCGA 
                   
               
               
                   
                 CGCCAUCGUGAGGCACGGCGGCAGGGAGGAGGACAUCAC 
                   
               
               
                   
                 CCUGGUGCGGGUGCCCGGCAGCUGGGAGAUCCCCGUGGC 
                   
               
               
                   
                 CGCCGGCGAGCUGGCCAGGAAGGAAGAUAUUGAUGCAGU 
                   
               
               
                   
                 GAUCGCCAUCGGCGUGCUGAUCAGGGGCGCCACCCCACAC 
                   
               
               
                   
                 UUCGACUACAUCGCCAGCGAAGUGAGCAAGGGACUGGCG 
                   
               
               
                   
                 GACCUGAGCCUGGAGCUGAGGAAGCCCAUCACCUUCGGC 
                   
               
               
                   
                 GUGAUCACCGCCGACACCCUGGAGCAGGCCAUCGAGAGG 
                   
               
               
                   
                 GCCGGCACCAAGCACGGCAACAAGGGAUGGGAGGCCGCC 
                   
               
               
                   
                 CUGAGCGCUAUCGAAAUGGCCAACCUCUUCAAGAGCCUG 
                   
               
               
                   
                 AGG 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MDSKGSSQKGSRLLLLLVVSNLLLPQGVVEAALLVCQYTIQS 
                  88 
               
               
                 amino acid 
                 LIHLTGEDPGFFNVEIPEFPFYPTCNVCTADVNVTINFDVGGKK 
                   
               
               
                 sequence 
                 HQLDLDFGQLTPHTKAVYQPRGAFGGSENATNLFLLELLGAG 
                   
               
               
                   
                 ELALTMRSKKLPINVTTGEEQQVSLESVDVYFQDVFGTMWCH 
                   
               
               
                   
                 HAEMQNPVYLIPETVPYIKWDNCNSTNITAVVRAQGLDVTLP 
                   
               
               
                   
                 LSLPTSAQDSNFSVKTEMLGNEIDIECIMEDGEISQVLPGDNKF 
                   
               
               
                   
                 NITCSGYESHVPSGGILTSTSPVATPIPGTGYAYSLRLTPRPVSR 
                   
               
               
                   
                 FLGNNSILYVFYSGNGPKASGGDYCIQSNIVFSDEIPASQDMPT 
                   
               
               
                   
                 NTTDITYVGDNATYSVPMVTSEDANSPNVTVTAFWAWPNNT 
                   
               
               
                   
                 ETDFKCKWTLTSGTPSGCENISGAFASNRTFDITVSGLGTAPKT 
                   
               
               
                   
                 LIITRTATNATTTTHKVIFSKAPGSGSAGSASGGASSGSGGASM 
                   
               
               
                   
                 QIYEGKLTAEGLRFGIVASRFNHALVDRLVEGAIDAIVRHGGR 
                   
               
               
                   
                 EEDITLVRVPGSWEIPVAAGELARKEDIDAVIAIGVLIRGATPH 
                   
               
               
                   
                 FDYIASEVSKGLADLSLELRKPITFGVITADTLEQAIERAGTKH 
                   
               
               
                   
                 GNKGWEAALSAIEMANLFKSLR 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 gp350_D123_HBsAg_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 191 consists of from 5′ end to 3′ end: 
                 191 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 89, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGACAGCAAGGGCAGCAGCCAGAAGGGCAGUAGGCUC 
                  89 
               
               
                 Construct 
                 CUUUUGCUACUUGUGGUGAGCAACCUCUUGUUACCCCAG 
                   
               
               
                 (excluding 
                 GGCGUGGUGGAGGCAGCACUCCUCGUCUGCCAGUAUACA 
                   
               
               
                 the stop 
                 AUCCAGUCGCUCAUUCAUUUGACAGGCGAGGACCCCGGC 
                   
               
               
                 codon) 
                 UUCUUUAACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCA 
                   
               
               
                   
                 ACCUGUAACGUUUGCACGGCAGACGUGAACGUGACAAUC 
                   
               
               
                   
                 AAUUUCGACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUG 
                   
               
               
                   
                 GAUUUCGGCCAACUGACGCCACACACCAAAGCGGUGUAC 
                   
               
               
                   
                 CAACCAAGGGGUGCAUUUGGAGGGAGCGAGAAUGCUACA 
                   
               
               
                   
                 AAUCUGUUCUUACUGGAACUGCUUGGUGCAGGAGAGCUG 
                   
               
               
                   
                 GCACUAACCAUGCGAAGCAAGAAGCUCCCCAUCAACGUU 
                   
               
               
                   
                 ACCACCGGGGAAGAACAGCAAGUCAGCCUUGAAUCCGUG 
                   
               
               
                   
                 GAUGUGUACUUUCAGGAUGUUUUCGGCACCAUGUGGUGU 
                   
               
               
                   
                 CACCACGCUGAGAUGCAGAAUCCCGUGUAUCUCAUACCC 
                   
               
               
                   
                 GAGACUGUACCCUACAUCAAGUGGGAUAAUUGCAAUUCA 
                   
               
               
                   
                 ACUAAUAUUACGGCUGUAGUGAGGGCCCAGGGCUUGGAC 
                   
               
               
                   
                 GUGACUCUGCCCUUAUCUCUACCUACUUCUGCCCAAGACU 
                   
               
               
                   
                 CCAAUUUCUCUGUCAAGACCGAGAUGCUCGGGAAUGAGA 
                   
               
               
                   
                 UCGAUAUCGAGUGCAUCAUGGAGGACGGUGAGAUAAGCC 
                   
               
               
                   
                 AGGUUCUGCCCGGCGACAACAAGUUCAAUAUCACUUGUU 
                   
               
               
                   
                 CUGGCUACGAGUCCCAUGUGCCUAGUGGUGGCAUACUCA 
                   
               
               
                   
                 CAAGUACUUCUCCCGUAGCCACGCCCAUUCCCGGAACCGG 
                   
               
               
                   
                 AUACGCCUACAGUCUGCGUCUGACCCCACGGCCUGUGUCC 
                   
               
               
                   
                 AGAUUCCUGGGUAACAAUAGUAUCUUAUACGUGUUUUAU 
                   
               
               
                   
                 AGCGGAAACGGCCCUAAAGCGUCCGGAGGGGACUAUUGU 
                   
               
               
                   
                 AUUCAGAGUAAUAUCGUUUUCUCUGAUGAGAUUCCAGCC 
                   
               
               
                   
                 AGUCAGGACAUGCCGACAAACACAACUGAUAUUACCUAC 
                   
               
               
                   
                 GUGGGCGACAAUGCCACGUAUUCAGUGCCCAUGGUCACG 
                   
               
               
                   
                 AGCGAGGACGCCAAUUCACCAAAUGUUACUGUAACAGCU 
                   
               
               
                   
                 UUCUGGGCCUGGCCAAAUAACACUGAGACUGACUUCAAA 
                   
               
               
                   
                 UGUAAGUGGACUUUGACCUCUGGAACUCCGUCGGGUUGC 
                   
               
               
                   
                 GAGAAUAUCAGCGGGGCCUUUGCUUCCAACAGGACUUUC 
                   
               
               
                   
                 GACAUCACUGUCUCAGGGCUGGGGACAGCACCGAAGACA 
                   
               
               
                   
                 UUAAUCAUAACACGGACCGCCACCAACGCCACGACUACAA 
                   
               
               
                   
                 CCCAUAAGGUGAUCUUUUCCAAGGCACCUGGCAGCGGCA 
                   
               
               
                   
                 GCGCUGGCUCCGCUAGCGGCGGCGCCUCUAGCGGAUCAG 
                   
               
               
                   
                 GAGGCGCAUCCAUGGAGAACAUUGCAUCUGGCCUGCUGG 
                   
               
               
                   
                 GACCUCUACUGGUGCUGCAGGCCGGAUUCUUCCUGCUGA 
                   
               
               
                   
                 CCAAGAUCCUGACCAUCCCUCAGAGCCUGGACAGCUGGU 
                   
               
               
                   
                 GGACCAGCCUGAACUUCCUGGGCGGCACCCCAGUGUGCCU 
                   
               
               
                   
                 GGGCCAGAACAGCCAGAGCCAGAUCUCCUCCCACAGCCCC 
                   
               
               
                   
                 ACCUGCUGUCCGCCCAUCUGCCCCGGCUACCGUUGGAUGU 
                   
               
               
                   
                 GCCUGAGGAGGUUCAUCAUCUUCCUGUGCAUACUCCUGU 
                   
               
               
                   
                 UGUGUCUAAUUUUCCUGCUCGUUCUGCUCGACUACCAGG 
                   
               
               
                   
                 GCAUGCUGCCGGUGUGUCCUCUGAUCCCCGGCUCCUCCAC 
                   
               
               
                   
                 CACCAGCACCGGCCCCUGCAGGACCUGCACCACACCAGCA 
                   
               
               
                   
                 CAAGGUACCAGCAUGUUCCCCAGCUGUUGCUGUACCAAG 
                   
               
               
                   
                 CCCACCGACGGCAACUGCACCUGCAUCCCCAUCCCCAGCA 
                   
               
               
                   
                 GCUGGGCCUUCGCCAAGUACCUGUGGGAGUGGGCCAGCG 
                   
               
               
                   
                 UGAGGUUCAGCUGGCUGUCACUUCUUGCGCCAUUCGUGC 
                   
               
               
                   
                 AGUGGUUCGUGGGCCUGAGCCCUACUGUGUGGCUGUCGG 
                   
               
               
                   
                 UAAUCUGGAUGAUGUGGUUCUGGGGCCCCAGCCUGUACA 
                   
               
               
                   
                 ACAUCCUGUCACCGUUCAUGCCUCUCCUACCGCUGUUCUU 
                   
               
               
                   
                 CUGCCUGUGGGUGUACAUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MDSKGSSQKGSRLLLLLVVSNLLLPQGVVEAALLVCQYTIQS 
                  90 
               
               
                 amino acid 
                 LIHLTGEDPGFFNVEIPEFPFYPTCNVCTADVNVTINFDVGGKK 
                   
               
               
                 sequence 
                 HQLDLDFGQLTPHTKAVYQPRGAFGGSENATNLFLLELLGAG 
                   
               
               
                   
                 ELALTMRSKKLPINVTTGEEQQVSLESVDVYFQDVFGTMWCH 
                   
               
               
                   
                 HAEMQNPVYLIPETVPYIKWDNCNSTNITAVVRAQGLDVTLP 
                   
               
               
                   
                 LSLPTSAQDSNFSVKTEMLGNEIDIECIMEDGEISQVLPGDNKF 
                   
               
               
                   
                 NITCSGYESHVPSGGILTSTSPVATPIPGTGYAYSLRLTPRPVSR 
                   
               
               
                   
                 FLGNNSILYVFYSGNGPKASGGDYCIQSNIVFSDEIPASQDMPT 
                   
               
               
                   
                 NTTDITYVGDNATYSVPMVTSEDANSPNVTVTAFWAWPNNT 
                   
               
               
                   
                 ETDFKCKWTLTSGTPSGCENISGAFASNRTFDITVSGLGTAPKT 
                   
               
               
                   
                 LIITRTATNATTTTHKVIFSKAPGSGSAGSASGGASSGSGGASM 
                   
               
               
                   
                 ENIASGLLGPLLVLQAGFFLLTKILTIPQSLDSWWTSLNFLGGT 
                   
               
               
                   
                 PVCLGQNSQSQISSHSPTCCPPICPGYRWMCLRRFIIFLCILLLC 
                   
               
               
                   
                 LIFLLVLLDYQGMLPVCPLIPGSSTTSTGPCRTCTTPAQGTSMF 
                   
               
               
                   
                 PSCCCTKPTDGNCTCIPIPSSWAFAKYLWEWASVRFSWLSLLA 
                   
               
               
                   
                 PFVQWFVGLSPTVWLSVIWMMWFWGPSLYNILSPFMPLLPLF 
                   
               
               
                   
                 FCLWVYI 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 gp350 D123_HBcAg_heterotandem_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 192 consists of from 5′ end to 3′ end: 
                 192 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 91, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGACAGCAAGGGCAGCAGCCAGAAGGGAUCCAGGCUA 
                  91 
               
               
                 Construct 
                 UUGUUACUAUUAGUGGUGAGCAACCUCCUCUUGCCCCAG 
                   
               
               
                 (excluding 
                 GGCGUGGUGGACAUCGACCCCUACAAGGAGUUCGGCGCC 
                   
               
               
                 the stop 
                 ACCGUGGAGUUGUUAAGCUUCCUCCCCAGCGACUUCUUC 
                   
               
               
                 codon) 
                 CCCAGCGUGAGGGACCUGCUGGACACCGCCGCCGCCCUGU 
                   
               
               
                   
                 ACAGGGAGGCCCUGGAGAGCCCCGAGCACUGCUCGCCUCA 
                   
               
               
                   
                 CCACACCGCCCUGAGGCAGGCCAUCCUGUGCUGGGGCGAG 
                   
               
               
                   
                 CUGAUGACCCUGGCCACCUGGGUGGGCAACAACCUGGAG 
                   
               
               
                   
                 GACCCCGCCAGCCGCGACCUCGUUGUGAACUACGUGAACA 
                   
               
               
                   
                 CCAACAUGGGCCUGAAGAUCAGGCAGCUGCUGUGGUUCC 
                   
               
               
                   
                 ACAUCAGCUGCCUGACCUUCGGCAGGGAGACAGUGCUGG 
                   
               
               
                   
                 AGUACCUGGUGAGCUUCGGCGUGUGGAUCAGGACUCCAC 
                   
               
               
                   
                 CCGCCUACAGGCCUCCCAACGCGCCAAUCCUGAGCACCCU 
                   
               
               
                   
                 GCCCGAGACAACAGUGGUGGGCAGCGGCAGCGCAGGUUC 
                   
               
               
                   
                 CGCCAGCGGCGGCGCAUCCAGUGGCUCUGGAGGAGCUUC 
                   
               
               
                   
                 UGACAUUGACCCAUAUAAGGAAUUCGGUGCUACAGUAGA 
                   
               
               
                   
                 GCUACUGUCUUUCUUGCCUAGUGAUUUCUUCCCGAGUGU 
                   
               
               
                   
                 AAGGGAUCUCCUAGAUACAGCCGCUGCUCUAUAUAGAGA 
                   
               
               
                   
                 GGCACUGGAAUCUCCAGAGCACUGUUCCCCACACCAUACC 
                   
               
               
                   
                 GCUCUCCGGCAGGCAAUCCUGUGUUGGGGUGAACUUAUG 
                   
               
               
                   
                 ACACUGGCAACCUGGGUCGGUAACAACGGAUCAGGUGAG 
                   
               
               
                   
                 GCGGCUCUGCUGGUCUGCCAGUAUACAAUCCAGUCGCUC 
                   
               
               
                   
                 AUUCAUCUGACAGGCGAAGAUCCAGGUUUCUUUAACGUG 
                   
               
               
                   
                 GAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGUAACGUU 
                   
               
               
                   
                 UGUACAGCUGACGUGAACGUGACAAUCAAUUUCGACGUG 
                   
               
               
                   
                 GGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUCGGCCAA 
                   
               
               
                   
                 CUGACGCCACACACCAAAGCGGUGUACCAACCAAGGGGU 
                   
               
               
                   
                 GCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGUUCUUA 
                   
               
               
                   
                 CUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAACCAUG 
                   
               
               
                   
                 CGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGGGGAA 
                   
               
               
                   
                 GAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUACUUU 
                   
               
               
                   
                 CAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGCUGAG 
                   
               
               
                   
                 AUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGUACCC 
                   
               
               
                   
                 UACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAUUACG 
                   
               
               
                   
                 GCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCUGCCC 
                   
               
               
                   
                 UUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUCUCU 
                   
               
               
                   
                 GUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUCGAG 
                   
               
               
                   
                 UGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUGCCC 
                   
               
               
                   
                 GGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUACGAG 
                   
               
               
                   
                 UCCCAUGUGCCUUCUGGUGGCAUACUCACAAGUACUUCU 
                   
               
               
                   
                 CCCGUAGCCACGCCCAUUCCCGGAACCGGAUAUGCGUAUA 
                   
               
               
                   
                 GUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCUGG 
                   
               
               
                   
                 GUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAACG 
                   
               
               
                   
                 GCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAGUA 
                   
               
               
                   
                 AUAUCGUUUUCUCUGAUGAAAUUCCUGCCAGUCAGGACA 
                   
               
               
                   
                 UGCCGACAAACACAACUGAUAUUACCUACGUGGGCGACA 
                   
               
               
                   
                 AUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGACG 
                   
               
               
                   
                 CCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGCCU 
                   
               
               
                   
                 GGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUGGA 
                   
               
               
                   
                 CUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAUCA 
                   
               
               
                   
                 GCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCACUG 
                   
               
               
                   
                 UCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAUAA 
                   
               
               
                   
                 CACGGACCGCCACCAACGCCACGACUACAACCCAUAAGGU 
                   
               
               
                   
                 GAUCUUUUCCAAGGCACCUGGAUCCGGACUGGUGGUCAA 
                   
               
               
                   
                 CUAUGUGAAUACAAAUAUGGGCCUCAAGAUUAGGCAGCU 
                   
               
               
                   
                 CCUAUGGUUCCAUAUUUCCUGCUUAACUUUCGGUAGGGA 
                   
               
               
                   
                 AACUGUCCUGGAAUAUCUCGUUAGCUUCGGAGUGUGGAU 
                   
               
               
                   
                 UCGGACUCCUCCUGCCUACAGACCACCUAACGCUCCAAUU 
                   
               
               
                   
                 CUGUCAACUCUCCCAGAGACGACUGUGGUCAGAAGGAGG 
                   
               
               
                   
                 GGCAGGAGCCCCAGACGGCGGACACCAAGCCCUCGCCGAA 
                   
               
               
                   
                 GAAGAAGCCAAAGCCCGCGGCGCCGGAGAUCUCAGUCCA 
                   
               
               
                   
                 GAGAGAGCCAGUGC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MDSKGSSQKGSRLLLLLVVSNLLLPQGVVDIDPYKEFGATVE 
                  92 
               
               
                 amino acid 
                 LLSFLPSDFFPSVRDLLDTAAALYREALESPEHCSPHHTALRQ 
                   
               
               
                 sequence 
                 AILCWGELMTLATWVGNNLEDPASRDLVVNYVNTNMGLKIR 
                   
               
               
                   
                 QLLWFHISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNAPILST 
                   
               
               
                   
                 LPETTVVGSGSAGSASGGASSGSGGASDIDPYKEFGATVELLS 
                   
               
               
                   
                 FLPSDFFPSVRDLLDTAAALYREALESPEHCSPHHTALRQAILC 
                   
               
               
                   
                 WGELMTLATWVGNNGSGEAALLVCQYTIQSLIHLTGEDPGFF 
                   
               
               
                   
                 NVEIPEFPFYPTCNVCTADVNVTINFDVGGKKHQLDLDFGQLT 
                   
               
               
                   
                 PHTKAVYQPRGAFGGSENATNLFLLELLGAGELALTMRSKKL 
                   
               
               
                   
                 PINVTTGEEQQVSLESVDVYFQDVFGTMWCHHAEMQNPVYLI 
                   
               
               
                   
                 PETVPYIKWDNCNSTNITAVVRAQGLDVTLPLSLPTSAQDSNF 
                   
               
               
                   
                 SVKTEMLGNEIDIECIMEDGEISQVLPGDNKFNITCSGYESHVP 
                   
               
               
                   
                 SGGILTSTSPVATPIPGTGYAYSLRLTPRPVSRFLGNNSILYVFY 
                   
               
               
                   
                 SGNGPKASGGDYCIQSNIVFSDEIPASQDMPTNTTDITYVGDN 
                   
               
               
                   
                 ATYSVPMVTSEDANSPNVTVTAFWAWPNNTETDFKCKWTLT 
                   
               
               
                   
                 SGTPSGCENISGAFASNRTFDITVSGLGTAPKTLIITRTATNATT 
                   
               
               
                   
                 TTHKVIFSKAPGSGLVVNYVNTNMGLKIRQLLWFHISCLTFGR 
                   
               
               
                   
                 ETVLEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVVRRRGRSP 
                   
               
               
                   
                 RRRTPSPRRRRSQSPRRRRSQSRESQC 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350 Ecto_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 193 consists of from 5′ end to 3′ end: 
                 193 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 93, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORFofmRNA 
                 AUGGACAGCAAGGGCAGCAGCCAGAAGGGUAGCAGGCUA 
                  93 
               
               
                 Construct 
                 CUCUUGCUCCUAGUGGUGAGCAACUUGCUUCUUCCCCAG 
                   
               
               
                 (excluding 
                 GGCGUGGUGGAGGCAGCAUUGCUUGUCUGCCAGUAUACA 
                   
               
               
                 the stop 
                 AUCCAGUCGCUCAUUCAUCUAACAGGCGAGGACCCCGGC 
                   
               
               
                 codon) 
                 UUCUUUAACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCA 
                   
               
               
                   
                 ACCUGUAACGUUUGCACUGCCGACGUGAACGUGACAAUC 
                   
               
               
                   
                 AAUUUCGACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUG 
                   
               
               
                   
                 GAUUUCGGCCAACUGACGCCACACACCAAAGCGGUGUAC 
                   
               
               
                   
                 CAACCAAGGGGUGCAUUUGGAGGGAGCGAGAAUGCUACA 
                   
               
               
                   
                 AAUCUGUUCUUACUGGAACUGCUUGGUGCAGGAGAGCUG 
                   
               
               
                   
                 GCACUAACCAUGCGAAGCAAGAAGCUCCCCAUCAACGUU 
                   
               
               
                   
                 ACCACCGGGGAAGAACAGCAAGUCAGCCUUGAAUCCGUG 
                   
               
               
                   
                 GAUGUGUACUUUCAGGAUGUUUUCGGCACCAUGUGGUGU 
                   
               
               
                   
                 CACCACGCUGAGAUGCAGAAUCCCGUGUAUCUCAUACCC 
                   
               
               
                   
                 GAGACUGUACCCUACAUCAAGUGGGAUAAUUGCAAUUCA 
                   
               
               
                   
                 ACUAAUAUUACGGCUGUGGUGAGGGCCCAGGGCUUGGAC 
                   
               
               
                   
                 GUGACUCUGCCCUUAUCUCUACCUACUUCUGCCCAAGACU 
                   
               
               
                   
                 CCAAUUUCUCUGUCAAGACCGAGAUGCUCGGGAAUGAGA 
                   
               
               
                   
                 UCGAUAUCGAGUGCAUCAUGGAGGACGGUGAGAUAAGCC 
                   
               
               
                   
                 AGGUUCUGCCCGGCGACAACAAGUUCAAUAUCACUUGUU 
                   
               
               
                   
                 CUGGCUACGAGUCCCAUGUGCCUUCCGGCGGCAUACUCAC 
                   
               
               
                   
                 AAGUACUUCUCCCGUAGCCACGCCCAUUCCCGGAACCGGA 
                   
               
               
                   
                 UACGCCUACAGUCUGCGUCUGACCCCACGGCCUGUGUCCA 
                   
               
               
                   
                 GAUUCCUGGGUAACAAUAGUAUCUUAUACGUGUUUUAUA 
                   
               
               
                   
                 GCGGAAACGGCCCUAAAGCGUCCGGAGGGGACUAUUGUA 
                   
               
               
                   
                 UUCAGAGUAAUAUCGUUUUCUCUGAUGAGAUUCCAGCCA 
                   
               
               
                   
                 GUCAGGACAUGCCGACAAACACAACUGAUAUUACCUACG 
                   
               
               
                   
                 UGGGCGACAAUGCCACGUAUUCAGUGCCCAUGGUCACGA 
                   
               
               
                   
                 GCGAGGACGCCAAUUCACCAAAUGUUACUGUAACAGCUU 
                   
               
               
                   
                 UCUGGGCCUGGCCAAAUAACACUGAGACUGACUUCAAAU 
                   
               
               
                   
                 GUAAGUGGACUUUGACCUCUGGAACUCCGUCGGGUUGCG 
                   
               
               
                   
                 AGAAUAUCAGCGGGGCCUUUGCUUCCAACAGGACUUUCG 
                   
               
               
                   
                 ACAUCACUGUCUCAGGGCUGGGGACAGCACCGAAGACAU 
                   
               
               
                   
                 UAAUCAUAACACGGACCGCCACCAACGCCACGACUACAAC 
                   
               
               
                   
                 CCAUAAGGUGAUCUUUUCCAAGGCACCUGAGUCCACCAC 
                   
               
               
                   
                 UACCUCCCCGACUCUUAACACUACGGGCUUCGCUGAUCCC 
                   
               
               
                   
                 AAUACUACUACUGGGUUGCCUAGCUCGACACAUGUGCCG 
                   
               
               
                   
                 ACGAACCUGACUGCCCCUGCAUCGACCGGGCCCACAGUUU 
                   
               
               
                   
                 CGACCGCCGAUGUGACAAGCCCUACGCCCGCAGGUACAAC 
                   
               
               
                   
                 CUCAGGCGCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGG 
                   
               
               
                   
                 GACAACGGAACGGAGUCCAAGGCUCCUGAUAUGACUUCC 
                   
               
               
                   
                 UCUACCAGCCCCGUGACUACACCCACUCCCAACGCAACUA 
                   
               
               
                   
                 GCCCAACCCCAGCUGUGACGACGCCCACCCCGAACGCGAC 
                   
               
               
                   
                 AUCUCCCACACCUGCUGUGACAACCCCUACCCCUAACGCC 
                   
               
               
                   
                 ACUAGCCCUACCCUAGGUAAGACCAGUCCGACUAGCGCCG 
                   
               
               
                   
                 UUACUACACCGACCCCUAAUGCAACCGGCCCGACUGUCGG 
                   
               
               
                   
                 CGAGACUUCCCCGCAAGCCAAUGCGACAAAUCACACAUU 
                   
               
               
                   
                 GGGCGGGACCUCUCCUACACCAGUCGUUACAUCUCAGCCU 
                   
               
               
                   
                 AAGAACGCUACCUCCGCUGUCACUACCGGCCAGCACAACA 
                   
               
               
                   
                 UCACAAGCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGA 
                   
               
               
                   
                 GCUCAAAUCCCGAGACACUAUCACCAUCCACAUCAGACAA 
                   
               
               
                   
                 CAGUACUUCACACAUGCCACUCUUGACGAGCGCUCACCCC 
                   
               
               
                   
                 ACCGGCGGCGAGAACAUUACCCAGGUGACUCCGGCGUCU 
                   
               
               
                   
                 AUUUCCACCCACCACGUCAGCACGUCGUCUCCCGCACCAC 
                   
               
               
                   
                 GGCCAGGGACGACUUCUCAGGCCAGCGGCCCAGGAAACU 
                   
               
               
                   
                 CCUCUACAAGCACAAAGCCAGGCGAAGUUAACGUCACCA 
                   
               
               
                   
                 AGGGAACGCCUCCCCAGAACGCAACCUCCCCACAGGCUCC 
                   
               
               
                   
                 CAGCGGGCAGAAGACUGCGGUGCCAACUGUGACCAGCAC 
                   
               
               
                   
                 CGGUGGCAAGGCCAACUCAACAACUGGAGGCAAGCAUAC 
                   
               
               
                   
                 GACGGGGCACGGCGCCCGGACCUCCACUGAACCCACGACC 
                   
               
               
                   
                 GAUUACGGAGGUGAUAGUACCACCCCGCGGCCACGAUAU 
                   
               
               
                   
                 AAUGCCACCACUUAUCUGCCACCUAGCACUUCCUCCAAGC 
                   
               
               
                   
                 UCCGGCCACGGUGGACCUUCACAAGCCCACCCGUGACGAC 
                   
               
               
                   
                 AGCCCAGGCGACGGUGCCAGUCCCACCUACAAGCCAGCCA 
                   
               
               
                   
                 CGCUUCUCCAACCUUAGU 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MDSKGSSQKGSRLLLLLVVSNLLLPQGVVEAALLVCQYTIQS 
                  94 
               
               
                 amino acid 
                 LIHLTGEDPGFFNVEIPEFPFYPTCNVCTADVNVTINFDVGGKK 
                   
               
               
                 sequence 
                 HQLDLDFGQLTPHTKAVYQPRGAFGGSENATNLFLLELLGAG 
                   
               
               
                   
                 ELALTMRSKKLPINVTTGEEQQVSLESVDVYFQDVFGTMWCH 
                   
               
               
                   
                 HAEMQNPVYLIPETVPYIKWDNCNSTNITAVVRAQGLDVTLP 
                   
               
               
                   
                 LSLPTSAQDSNFSVKTEMLGNEIDIECIMEDGEISQVLPGDNKF 
                   
               
               
                   
                 NITCSGYESHVPSGGILTSTSPVATPIPGTGYAYSLRLTPRPVSR 
                   
               
               
                   
                 FLGNNSILYVFYSGNGPKASGGDYCIQSNIVFSDEIPASQDMPT 
                   
               
               
                   
                 NTTDITYVGDNATYSVPMVTSEDANSPNVTVTAFWAWPNNT 
                   
               
               
                   
                 ETDFKCKWTLTSGTPSGCENISGAFASNRTFDITVSGLGTAPKT 
                   
               
               
                   
                 LIITRTATNATTTTHKVIFSKAPESTTTSPTLNTTGFADPNTTTG 
                   
               
               
                   
                 LPSSTHVPTNLTAPASTGPTVSTADVTSPTPAGTTSGASPVTPS 
                   
               
               
                   
                 PSPWDNGTESKAPDMTSSTSPVTTPTPNATSPTPAVTTPTPNAT 
                   
               
               
                   
                 SPTPAVTTPTPNATSPTLGKTSPTSAVTTPTPNATGPTVGETSP 
                   
               
               
                   
                 QANATNHTLGGTSPTPVVTSQPKNATSAVTTGQHNITSSSTSS 
                   
               
               
                   
                 MSLRPSSNPETLSPSTSDNSTSHMPLLTSAHPTGGENITQVTPA 
                   
               
               
                   
                 SISTHHVSTSSPAPRPGTTSQASGPGNSSTSTKPGEVNVTKGTP 
                   
               
               
                   
                 PQNATSPQAPSGQKTAVPTVTSTGGKANSTTGGKHTTGHGAR 
                   
               
               
                   
                 TSTEPTTDYGGDSTTPRPRYNATTYLPPSTSSKLRPRWTFTSPP 
                   
               
               
                   
                 VTTAQATVPVPPTSQPRFSNLS 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gL-gH_LS_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 194 consists of from 5′ end to 3′ end: 
                 194 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 95, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGACUCGAAGGGAUCUAGUCAGAAAGGCUCUAGGCUC 
                  95 
               
               
                 Construct 
                 CUACUCCUCUUGGUGGUGAGCAACUUACUCCUUCCCCAG 
                   
               
               
                 (excluding 
                 GGCGUGGUGAGAGCAGUGGGCGUUUUCUUAGCAAUCUGC 
                   
               
               
                 the stop 
                 CUCGUGACAAUCUUUGUGUUGCCGACCUGGGGCAACUGG 
                   
               
               
                 codon) 
                 GCCUACCCCUGCUGCCACGUGACCCAGCUGAGAGCUCAAC 
                   
               
               
                   
                 ACCUGCUUGCCCUGGAGAACAUCAGCGACAUCUACCUUG 
                   
               
               
                   
                 UAAGCAACCAGACAUGCGACGGCUUCAGUCUGGCCAGCC 
                   
               
               
                   
                 UGAACAGCCCCAAGAACGGCUCAAACCAGCUAGUGAUCA 
                   
               
               
                   
                 GCCGGUGCGCUAACGGUCUGAACGUUGUGAGCUUCUUCA 
                   
               
               
                   
                 UCAGCAUCUUGAAGAGAAGUAGUAGCGCGCUGACCGGCC 
                   
               
               
                   
                 ACCUGAGAGAGCUGCUGACCACUCUCGAGACACUCUAUG 
                   
               
               
                   
                 GCAGCUUCAGCGUGGAGGACCUGUUCGGCGCAAACCUGA 
                   
               
               
                   
                 ACCGCUACGCCUGGCACAGAGGCGGCGGAAGCGGUAGCG 
                   
               
               
                   
                 CUGGCAGCGCCAGCGGAGGCGCUUCAUCCGGAUCCGGCG 
                   
               
               
                   
                 GCGCAAGCAGCCUUUCUGAGGUGAAGCUCCACUUGGACA 
                   
               
               
                   
                 UCGAAGGCCACGCCAGCCAUUACACCAUACCAUGGACCGA 
                   
               
               
                   
                 GCUGAUGGCAAAGGUGCCCGGCCUUAGCCCAGAAGCCCU 
                   
               
               
                   
                 GUGGCGAGAGGCCAACGUUACCGAAGACUUGGCUAGUAU 
                   
               
               
                   
                 GUUAAACAGGUACAAACUUAUCUACAAGACUAGCGGCAC 
                   
               
               
                   
                 CCUGGGCAUCGCAUUAGCCGAGCCCGUGGACAUCCCCGCC 
                   
               
               
                   
                 GUUAGCGAGGGCAGCAUGCAGGUGGACGCCAGCAAGGUU 
                   
               
               
                   
                 CACCCUGGUGUAAUUAGUGGCCUCAACAGCCCAGCCUGU 
                   
               
               
                   
                 AUGUUGAGCGCCCCACUGGAGAAGCAGCUGUUUUACUAU 
                   
               
               
                   
                 AUCGGGACCAUGUUGCCCAAUACCCGGCCACACAGUUAC 
                   
               
               
                   
                 GUGUUCUACCAGCUGCGGUGUCACCUGAGCUACGUGGCC 
                   
               
               
                   
                 CUGUCUAUCAACGGCGACAAGUUCCAGUACACCGGCGCC 
                   
               
               
                   
                 AUGACCAGCAAGUUCCUGAUGGGCACCUACAAGCGGGUC 
                   
               
               
                   
                 ACCGAGAAGGGCGACGAGCACGUGCUGUCACUUGUGUUC 
                   
               
               
                   
                 GGUAAGACCAAGGACCUGCCCGACCUUCGGGGCCCCUUU 
                   
               
               
                   
                 UCAUACCCUAGUUUGACCAGCGCCCAAAGUGGCGACUAC 
                   
               
               
                   
                 AGCCUUGUGAUAGUGACCACCUUCGUGCAUUACGCCAAC 
                   
               
               
                   
                 UUCCACAACUACUUCGUGCCCAACCUGAAGGACAUGUUC 
                   
               
               
                   
                 AGCCGGGCGGUAACCAUGACGGCCGCUUCUUAUGCCCGG 
                   
               
               
                   
                 UAUGUGCUGCAGAAGCUGGUCCUGCUGGAGAUGAAGGGC 
                   
               
               
                   
                 GGGUGCCGGGAGCCCGAGUUGGACACCGAAACCCUGACC 
                   
               
               
                   
                 ACCAUGUUCGAGGUUAGCGUGGCCUUCUUCAAGGUGGGU 
                   
               
               
                   
                 CACGCGGUGGGCGAAACCGGCAAUGGAUGCGUGGACUUA 
                   
               
               
                   
                 CGCUGGCUGGCCAAGAGUUUCUUUGAGCUUACCGUGCUG 
                   
               
               
                   
                 AAGGAUAUCAUCGGCAUCUGCUACGGCGCCACCGUAAAG 
                   
               
               
                   
                 GGAAUGCAGAGCUAUGGGCUGGAGAGACUUGCCGCCAUG 
                   
               
               
                   
                 CUCAUGGCUACCGUGAAGAUGGAGGAGCUGGGACACCUG 
                   
               
               
                   
                 ACAACAGAGAAGCAGGAGUACGCCCUGAGACUGGCCACC 
                   
               
               
                   
                 GUGGGCUACCCUAAGGCCGGCGUGUACAGUGGACUCAUA 
                   
               
               
                   
                 GGCGGAGCUACCAGCGUGCUCCUAUCCGCUUAUAACAGA 
                   
               
               
                   
                 CACCCCUUAUUUCAGCCCCUGCACACAGUGAUGCGGGAG 
                   
               
               
                   
                 ACUUUAUUCAUCGGAUCUCACGUCGUACUGCGGGAGUUG 
                   
               
               
                   
                 CGGCUGAACGUAACCACCCAGGGCCCUAAUUUAGCACUU 
                   
               
               
                   
                 UAUCAGCUGCUCAGUACUGCCCUGUGCAGCGCACUUGAG 
                   
               
               
                   
                 AUCGGCGAGGUACUCAGAGGCCUGGCCCUCGGUACGGAG 
                   
               
               
                   
                 AGUGGCCUGUUUUCUCCAUGCUACCUUAGCCUGCGGUUU 
                   
               
               
                   
                 GAUCUGACCAGAGACAAGUUGCUGAGCAUGGCUCCUCAA 
                   
               
               
                   
                 GAGGCGACCCUUGACCAGGCAGCCGUGUCAAACGCCGUG 
                   
               
               
                   
                 GACGGAUUCCUGGGCAGACUGUCUCUGGAACGGGAAGAC 
                   
               
               
                   
                 CGGGAUGCUUGGCACCUUCCUGCCUAUAAGUGUGUGGAU 
                   
               
               
                   
                 CGGCUGGAUAAGGUCCUGAUGAUAAUCCCUCUGAUUAAC 
                   
               
               
                   
                 GUUACUUUCAUCAUCUCUAGCGAUCGGGAGGUCCGAGGG 
                   
               
               
                   
                 UCCGCCCUCUACGAGGCCAGCACCACCUAUCUGAGCAGCA 
                   
               
               
                   
                 GCCUCUUCUUGUCUCCUGUGAUUAUGAACAAGUGCAGCC 
                   
               
               
                   
                 AGGGUGCAGUAGCCGGCGAGCCCCGGCAGAUCCCGAAGA 
                   
               
               
                   
                 GCGGCUUCGCCCUUCUGAGUUACGACGAGAAGGAGGGCC 
                   
               
               
                   
                 UGGAGACUACAACUUACAUCACAAGCCAGGAGGUGCAGA 
                   
               
               
                   
                 ACAGCAUCCUGUCAUCUAAUUACUUUGACUUCGACAACC 
                   
               
               
                   
                 UGCACGUUCAUUAUCUACUGCUAACAACUAACGGUACCG 
                   
               
               
                   
                 UCAUGGAAAUCGCAGGACUGUAUGAGGAGCGGGCCGGCA 
                   
               
               
                   
                 GCGGGAGCGCGGGCUCUGCCAGCGGUGGUGCCAGCAGCG 
                   
               
               
                   
                 GCAGUGGAGGUGCCUCCAUGCAGAUUUACGAAGGAAAGC 
                   
               
               
                   
                 UGACCGCCGAAGGCCUGAGGUUCGGCAUCGUGGCUUCCA 
                   
               
               
                   
                 GAUUCAACCACGCACUGGUGGACAGGCUGGUGGAGGGCG 
                   
               
               
                   
                 CCAUCGACGCAAUCGUGCGCCACGGCGGCAGGGAGGAGG 
                   
               
               
                   
                 ACAUCACCCUCGUAAGAGUCCCUGGUAGCUGGGAAAUUC 
                   
               
               
                   
                 CCGUGGCCGCGGGCGAGCUGGCUAGGAAGGAGGAUAUCG 
                   
               
               
                   
                 ACGCCGUCAUCGCCAUCGGCGUUCUCAUCCGGGGUGCCAC 
                   
               
               
                   
                 ACCACAUUUCGACUACAUCGCAAGCGAGGUUUCAAAGGG 
                   
               
               
                   
                 ACUCGCCGACUUAAGUCUGGAGCUGAGGAAGCCCAUCAC 
                   
               
               
                   
                 CUUUGGCGUGAUCACCGCCGACACCCUGGAGCAGGCCAUC 
                   
               
               
                   
                 GAGCGCGCCGGCACCAAGCACGGGAACAAGGGCUGGGAG 
                   
               
               
                   
                 GCAGCCUUAUCAGCCAUCGAAAUGGCGAAUCUGUUCAAG 
                   
               
               
                   
                 AGCCUGCGG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MDSKGSSQKGSRLLLLLVVSNLLLPQGVVRAVGVFLAICLVTI 
                  96 
               
               
                 amino acid 
                 FVLPTWGNWAYPCCHVTQLRAQHLLALENISDIYLVSNQTCD 
                   
               
               
                 sequence 
                 GFSLASLNSPKNGSNQLVISRCANGLNVVSFFISILKRSSSALTG 
                   
               
               
                   
                 HLRELLTTLETLYGSFSVEDLFGANLNRYAWHRGGGSGSAGS 
                   
               
               
                   
                 ASGGASSGSGGASSLSEVKLHLDIEGHASHYTIPWTELMAKVP 
                   
               
               
                   
                 GLSPEALWREANVTEDLASMLNRYKLIYKTSGTLGIALAEPV 
                   
               
               
                   
                 DIPAVSEGSMQVDASKVHPGVISGLNSPACMLSAPLEKQLFYY 
                   
               
               
                   
                 IGTMLPNTRPHSYVFYQLRCHLSYVALSINGDKFQYTGAMTS 
                   
               
               
                   
                 KFLMGTYKRVTEKGDEHVLSLVFGKTKDLPDLRGPFSYPSLT 
                   
               
               
                   
                 SAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMFSRAVTMTAA 
                   
               
               
                   
                 SYARYVLQKLVLLEMKGGCREPELDTETLTTMFEVSVAFFKV 
                   
               
               
                   
                 GHAVGETGNGCVDLRWLAKSFFELTVLKDIIGICYGATVKGM 
                   
               
               
                   
                 QSYGLERLAAMLMATVKMEELGHLTTEKQEYALRLATVGYP 
                   
               
               
                   
                 KAGVYSGLIGGATSVLLSAYNRHPLFQPLHTVMRETLFIGSHV 
                   
               
               
                   
                 VLRELRLNVTTQGPNLALYQLLSTALCSALEIGEVLRGLALGT 
                   
               
               
                   
                 ESGLFSPCYLSLRFDLTRDKLLSMAPQEATLDQAAVSNAVDG 
                   
               
               
                   
                 FLGRLSLEREDRDAWHLPAYKCVDRLDKVLMIIPLINVTFIISS 
                   
               
               
                   
                 DREVRGSALYEASTTYLSSSLFLSPVIMNKCSQGAVAGEPRQI 
                   
               
               
                   
                 PKIQNFTRTQKSCIFCGFALLSYDEKEGLETTTYITSQEVQNSIL 
                   
               
               
                   
                 SSNYFDFDNLHVHYLLLTTNGTVMEIAGLYEERAGSGSAGSA 
                   
               
               
                   
                 SGGASSGSGGASMQIYEGKLTAEGLRFGIVASRFNHALVDRL 
                   
               
               
                   
                 VEGAIDAIVRHGGREEDITLVRVPGSWEIPVAAGELARKEDID 
                   
               
               
                   
                 AVIAIGVLIRGATPHFDYIASEVSKGLADLSLELRKPITFGVITA 
                   
               
               
                   
                 DTLEQAIERAGTKHGNKGWEAALSAIEMANLFKSLR 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gBEcto_LS_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 195 consists of from 5′ end to 3′ end: 
                 195 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 97, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGACUCUAAGGGCAGCAGCCAGAAGGGCAGUAGGCUC 
                  97 
               
               
                 Construct 
                 CUACUUCUCCUCGUGGUGAGCAACUUAUUGCUUCCCCAG 
                   
               
               
                 (excluding 
                 GGAGUGGUGCAGACUCCGGAACAGCCCGCUCCGCCCGCUA 
                   
               
               
                 the stop 
                 CCACCGUCCAGCCUACCGCCACGCGCCAGCAGACUUCCUU 
                   
               
               
                 codon) 
                 CCCGUUUCGCGUCUGCGAGCUCUCCUCUCACGGCGAUCUC 
                   
               
               
                   
                 UUCCGCUUCUCCUCCGAUAUUCAGUGCCCCUCCUUCGGCA 
                   
               
               
                   
                 CCCGCGAGAACCAUACCGAGGGCCUCCUCAUGGUCUUUA 
                   
               
               
                   
                 AGGACAACAUCAUCCCUUACUCCUUCAAGGUACGCUCCU 
                   
               
               
                   
                 ACACAAAGAUAGUCACCAACAUCCUCAUCUAUAAUGGAU 
                   
               
               
                   
                 GGUACGCUGACUCUGUUACCAAUCGCCACGAGGAGAAGU 
                   
               
               
                   
                 UCUCCGUUGAUUCCUACGAGACUGACCAGAUGGAUACCA 
                   
               
               
                   
                 UCUACCAGUGCUACAAUGCCGUCAAGAUGACCAAGGACG 
                   
               
               
                   
                 GCCUGACCCGCGUCUAUGUCGACCGCGACGGCGUUAACA 
                   
               
               
                   
                 UAACUGUCAACCUCAAGCCUACAGGCGGCCUGGCCAAUG 
                   
               
               
                   
                 GCGUCAGACGCUACGCCUCCCAGACCGAGCUCUACGACGC 
                   
               
               
                   
                 UCCUGGCUGGCUGAUCUGGACCUACCGGACCCGCACCACU 
                   
               
               
                   
                 GUCAAUUGCCUCAUUACCGACAUGAUGGCCAAGUCCAAU 
                   
               
               
                   
                 AGCCCAUUCGAUUUCUUCGUAACCACCACCGGCCAGACCG 
                   
               
               
                   
                 UCGAGAUGUCGCCAUUCUAUGACGGCAAGAAUAAGGAAA 
                   
               
               
                   
                 CUUUCCACGAGAGAGCCGAUAGCUUCCACGUCCGCACGA 
                   
               
               
                   
                 ACUACAAGAUUGUCGACUACGACAAUCGCGGCACCAAUC 
                   
               
               
                   
                 CACAGGGCGAGCGCCGCGCCUUCCUGGACAAGGGCACCUA 
                   
               
               
                   
                 CACCCUCUCCUGGAAACUCGAGAACAGAACCGCCUACUGU 
                   
               
               
                   
                 CCCCUCCAGCACUGGCAGACUUUUGAUUCCACCAUCGCCA 
                   
               
               
                   
                 CUGAGACUGGCAAGAGUAUCCAUUUCGUCACCGACGAAG 
                   
               
               
                   
                 GAACAUCUUCAUUCGUCACAAAUACCACUGUGGGCAUUG 
                   
               
               
                   
                 AGCUCCCCGACGCCUUCAAGUGCAUCGAGGAACAGGUCA 
                   
               
               
                   
                 ACAAGACAAUGCACGAGAAGUACGAGGCCGUGCAGGACC 
                   
               
               
                   
                 GGUAUACCAAGGGCCAGGAGGCCAUUACAUACUUCAUCA 
                   
               
               
                   
                 CCAGCGGUGGAUUGCUUCUCGCCUGGCUUCCACUCACACC 
                   
               
               
                   
                 UCGCAGUCUGGCCACAGUUAAGAACCUCACUGAGUUGAC 
                   
               
               
                   
                 CACUCCAACAUCGUCACCACCUUCUUCACCUAGCCCUCCU 
                   
               
               
                   
                 GCUCCUUCCGCCGCCCGCGGCUCCACUCCUGCCGCUGUCU 
                   
               
               
                   
                 UAAGGGGCUCGGGCAGCGCCGGCAGCGCAUCGGGAGGGG 
                   
               
               
                   
                 CGAGUUCAGGGUCAGGCGGUGCCUCCGCAGGCAAUGCUA 
                   
               
               
                   
                 CCACACCAGUGCCACCAACCGCCCCUGGCAAGUCCUUAGG 
                   
               
               
                   
                 UACCCUGAACAAUCCAGCGACAGUUCAGAUCCAGUUCGC 
                   
               
               
                   
                 UUACGAUUCACUCAGGCGCCAGAUCAACCGCAUGCUUGG 
                   
               
               
                   
                 UGACCUUGCCCGGGCCUGGUGCCUCGAACAGAAGCGCCA 
                   
               
               
                   
                 GAACAUGGUACUUAGGGAACUUACCAAGAUUAAUCCUAC 
                   
               
               
                   
                 UACAGUCAUGUCCUCUAUCUACGGCAAGGCCGUUGCUGC 
                   
               
               
                   
                 CAAGCGCCUGGGCGACGUCAUCAGCGUGUCGCAGUGCGU 
                   
               
               
                   
                 CCCAGUCAACCAGGCUACCGUUACCCUCAGGAAGUCCAUG 
                   
               
               
                   
                 AGAGUGCCAGGCUCCGAAACAAUGUGCUAUUCCCGCCCU 
                   
               
               
                   
                 CUGGUUUCAUUUUCCUUCAUCAACGACACAAAGACCUAC 
                   
               
               
                   
                 GAGGGCCAGUUAGGAACUGACAAUGAGAUAUUCUUGACU 
                   
               
               
                   
                 AAGAAGAUGACUGAGGUAUGCCAGGCAACUUCUCAAUAC 
                   
               
               
                   
                 UACUUCCAGUCUGGAAAUGAAAUCCACGUAUAUAACGAC 
                   
               
               
                   
                 UAUCAUCACUUUAAGACUAUCGAACUCGACGGAAUUGCU 
                   
               
               
                   
                 ACACUCCAAACAUUCAUCUCACUGAAUACCUCCCUUAUCG 
                   
               
               
                   
                 AGAACAUCGACUUCGCAUCCCUCGAGCUGUAUAGCAGAG 
                   
               
               
                   
                 ACGAGCAGAGAGCCAGCAACGUGUUCGACCUGGAGGGUA 
                   
               
               
                   
                 UCUUUCGCGAGUACAACUUCCAGGCACAGAAUAUAGCCG 
                   
               
               
                   
                 GCCUCCGUAAGGACCUGGAUAACGCCGUGUCCAAUGGCC 
                   
               
               
                   
                 GCAACCAGUUCGUCGACGGUCUUGGCGAACUCAUGGACU 
                   
               
               
                   
                 CUCUGGGAUCCGUGGGGCAAUCCAUCACAAACUUAGUCU 
                   
               
               
                   
                 CUACUGUCGGUGGACUAUUCAGCAGCCUGGUUAGCGGAU 
                   
               
               
                   
                 UUAUCUCUUUCUUUAAGAACCCAGGCAGUGGUUCUGCCG 
                   
               
               
                   
                 GCUCAGCCAGCGGCGGCGCUAGUAGCGGCAGCGGAGGCG 
                   
               
               
                   
                 CCUCAAUGCAGAUCUAUGAGGGUAAGUUGACAGCCGAAG 
                   
               
               
                   
                 GCUUGAGGUUUGGCAUCGUAGCCAGCCGGUUCAACCACG 
                   
               
               
                   
                 CCCUGGUGGACAGGCUGGUUGAGGGUGCCAUUGACGCCA 
                   
               
               
                   
                 UUGUGAGGCACGGCGGCAGGGAGGAGGACAUUACCCUGG 
                   
               
               
                   
                 UCAGAGUCCCCGGAUCAUGGGAGAUCCCCGUGGCCGCCG 
                   
               
               
                   
                 GGGAGCUGGCCAGGAAGGAAGACAUCGACGCUGUGAUCG 
                   
               
               
                   
                 CCAUCGGCGUGCUGAUCCGAGGAGCCACACCCCAUUUCGA 
                   
               
               
                   
                 CUACAUCGCCAGUGAGGUUAGCAAAGGCCUAGCGGACCU 
                   
               
               
                   
                 GAGCCUGGAGCUGCGCAAGCCCAUCACCUUCGGCGUCAU 
                   
               
               
                   
                 UACUGCCGACACCUUGGAACAGGCCAUCGAAAGGGCCGG 
                   
               
               
                   
                 UACAAAGCACGGCAACAAGGGCUGGGAGGCAGCCCUUAG 
                   
               
               
                   
                 CGCCAUCGAGAUGGCAAACCUGUUUAAGUCCUUGAGG 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MDSKGSSQKGSRLLLLLVVSNLLLPQGVVQTPEQPAPPATTV 
                  98 
               
               
                 amino acid 
                 QPTATRQQTSFPFRVCELSSHGDLFRFSSDIQCPSFGTRENHTE 
                   
               
               
                 sequence 
                 GLLMVFKDNIIPYSFKVRSYTKIVTNILIYNGWYADSVTNRHE 
                   
               
               
                   
                 EKFSVDSYETDQMDTIYQCYNAVKMTKDGLTRVYVDRDGV 
                   
               
               
                   
                 NITVNLKPTGGLANGVRRYASQTELYDAPGWLIWTYRTRTTV 
                   
               
               
                   
                 NCLITDMMAKSNSPFDFFVTTTGQTVEMSPFYDGKNKETFHE 
                   
               
               
                   
                 RADSFHVRTNYKIVDYDNRGTNPQGERRAFLDKGTYTLSWK 
                   
               
               
                   
                 LENRTAYCPLQHWQTFDSTIATETGKSIHFVTDEGTSSFVTNT 
                   
               
               
                   
                 TVGIELPDAFKCIEEQVNKTMHEKYEAVQDRYTKGQEAITYFI 
                   
               
               
                   
                 TSGGLLLAWLPLTPRSLATVKNLTELTTPTSSPPSSPSPPAPSAA 
                   
               
               
                   
                 RGSTPAAVLRGSGSAGSASGGASSGSGGASAGNATTPVPPTAP 
                   
               
               
                   
                 GKSLGTLNNPATVQIQFAYDSLRRQINRMLGDLARAWCLEQK 
                   
               
               
                   
                 RQNMVLRELTKINPTTVMSSIYGKAVAAKRLGDVISVSQCVP 
                   
               
               
                   
                 VNQATVTLRKSMRVPGSETMCYSRPLVSFSFINDTKTYEGQL 
                   
               
               
                   
                 GTDNEIFLTKKMTEVCQATSQYYFQSGNEIHVYNDYHHFKTIE 
                   
               
               
                   
                 LDGIATLQTFISLNTSLIENIDFASLELYSRDEQRASNVFDLEGI 
                   
               
               
                   
                 FREYNFQAQNIAGLRKDLDNAVSNGRNQFVDGLGELMDSLG 
                   
               
               
                   
                 SVGQSITNLVSTVGGLFSSLVSGFISFFKNPGSGSAGSASGGAS 
                   
               
               
                   
                 SGSGGASMQIYEGKLTAEGLRFGIVASRFNHALVDRLVEGAID 
                   
               
               
                   
                 AIVRHGGREEDITLVRVPGSWEIPVAAGELARKEDIDAVIAIGV 
                   
               
               
                   
                 LIRGATPHFDYIASEVSKGLADLSLELRKPITFGVITADTLEQAI 
                   
               
               
                   
                 ERAGTKHGNKGWEAALSAIEMANLFKSLR 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350 A44Y V10 Stab 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 196 consists of from 5′ end to 3′ end: 
                 196 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 99, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                  99 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUUACGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTY 
                  45 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350 V15 Stab4_NGM6 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 197 consists of from 5′ end to 3′ end: 
                 197 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 100, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                 100 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUUACGACGUGAACGUGACAUUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUGCGAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAGCGAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 GCGGUCAAGACCGAGAUACUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCGCGUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUGCGGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCGCGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCUGG 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTY 
                  55 
               
               
                 amino acid 
                 DVNVTFNFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENAANLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSAN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFAVKTEILGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNIACSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVAVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNAATTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTWDVT 
                   
               
               
                   
                 SPTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTP 
                   
               
               
                   
                 NATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAV 
                   
               
               
                   
                 TTPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNAT 
                   
               
               
                   
                 SAVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLT 
                   
               
               
                   
                 SAHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNS 
                   
               
               
                   
                 STSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGK 
                   
               
               
                   
                 ANSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYL 
                   
               
               
                   
                 PPSTSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVL 
                   
               
               
                   
                 QWASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETY 
                   
               
               
                   
                 V 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V6_NGM N195_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 198 consists of from 5′ end to 3′ end: 
                 198 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 101, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                 101 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 GCGGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  65 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFAVKTEMLGNEIDIECI 
                   
               
               
                   
                 MEDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPG 
                   
               
               
                   
                 TGYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQ 
                   
               
               
                   
                 SNIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANS 
                   
               
               
                   
                 PNVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFAS 
                   
               
               
                   
                 NRTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTT 
                   
               
               
                   
                 SPTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVT 
                   
               
               
                   
                 SPTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTP 
                   
               
               
                   
                 NATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAV 
                   
               
               
                   
                 TTPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNAT 
                   
               
               
                   
                 SAVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLT 
                   
               
               
                   
                 SAHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNS 
                   
               
               
                   
                 STSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGK 
                   
               
               
                   
                 ANSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYL 
                   
               
               
                   
                 PPSTSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVL 
                   
               
               
                   
                 QWASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETY 
                   
               
               
                   
                 V 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V8_NGM N345_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 199 consists of from 5′ end to 3′ end: 
                 199 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 102, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                 102 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUGCGGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                 114 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVAVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V9_NGM N411_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 200 consists of from 5′ end to 3′ end: 
                 200 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 103, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                 103 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCGCGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  70 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNAATTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gH (BXLF2) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 201 consists of from 5′ end to 3′ end: 
                 201 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 106, and 
                   
               
               
                 3′ UTR SEQ ID NO: 106 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGCAGUUGUUGUGCGUGUUCUGCCUCGUGUUACUCUGG 
                 105 
               
               
                 Construct 
                 GAGGUGGGCGCCGCCAGCCUUAGCGAGGUGAAGCUCCAC 
                   
               
               
                 (excluding 
                 UUGGACAUCGAGGGCCACGCCAGCCACUACACCAUCCCCU 
                   
               
               
                 the stop 
                 GGACCGAGCUCAUGGCCAAGGUGCCCGGCCUUAGCCCCGA 
                   
               
               
                 codon) 
                 GGCCCUGUGGCGGGAGGCCAACGUGACCGAGGACCUGGC 
                   
               
               
                   
                 CAGCAUGCUGAACCGGUACAAGCUGAUCUACAAGACCAG 
                   
               
               
                   
                 CGGCACCCUGGGCAUCGCCCUGGCCGAGCCCGUGGACAUC 
                   
               
               
                   
                 CCCGCCGUUAGCGAAGGCAGCAUGCAGGUGGACGCCAGC 
                   
               
               
                   
                 AAGGUGCACCCCGGCGUGAUCAGCGGCCUGAACAGCCCCG 
                   
               
               
                   
                 CCUGUAUGUUGAGCGCCCCACUGGAGAAGCAGCUGUUCU 
                   
               
               
                   
                 ACUACAUCGGCACCAUGCUGCCCAACACCCGGCCCCACAG 
                   
               
               
                   
                 CUACGUGUUCUACCAGCUGCGGUGCCACCUGAGCUACGU 
                   
               
               
                   
                 UGCCCUGAGCAUCAACGGCGACAAGUUCCAGUACACCGG 
                   
               
               
                   
                 CGCCAUGACCAGCAAGUUCCUGAUGGGCACCUACAAGCG 
                   
               
               
                   
                 GGUCACCGAGAAGGGCGACGAGCACGUGCUGUCACUGGU 
                   
               
               
                   
                 GUUCGGCAAGACCAAGGACCUGCCCGACCUGCGGGGCCCC 
                   
               
               
                   
                 UUCAGCUACCCUAGUUUGACCAGCGCCCAGAGCGGCGAC 
                   
               
               
                   
                 UACAGCUUGGUGAUCGUGACCACCUUCGUGCACUACGCC 
                   
               
               
                   
                 AACUUCCACAACUACUUCGUGCCCAACCUGAAGGACAUG 
                   
               
               
                   
                 UUCAGCCGGGCCGUGACCAUGACUGCCGCUUCUUACGCCC 
                   
               
               
                   
                 GGUACGUGCUGCAGAAGCUGGUCCUGCUGGAGAUGAAGG 
                   
               
               
                   
                 GCGGCUGCCGGGAGCCCGAGCUGGACACCGAAACACUGA 
                   
               
               
                   
                 CCACCAUGUUCGAGGUGAGCGUGGCCUUCUUCAAGGUGG 
                   
               
               
                   
                 GUCACGCGGUGGGCGAAACCGGCAACGGCUGCGUGGACU 
                   
               
               
                   
                 UACGCUGGCUGGCCAAGAGCUUCUUCGAGCUGACCGUGC 
                   
               
               
                   
                 UGAAGGAUAUCAUCGGCAUCUGCUACGGCGCCACCGUGA 
                   
               
               
                   
                 AGGGCAUGCAGAGCUACGGCCUGGAGCGGCUGGCCGCCA 
                   
               
               
                   
                 UGCUUAUGGCAACAGUGAAGAUGGAGGAGCUGGGACACC 
                   
               
               
                   
                 UGACAACAGAGAAGCAGGAGUACGCCCUGAGACUGGCCA 
                   
               
               
                   
                 CAGUGGGCUACCCAAAGGCCGGCGUGUACAGUGGACUGA 
                   
               
               
                   
                 UCGGCGGCGCAACCAGCGUGCUGCUAUCCGCUUACAACCG 
                   
               
               
                   
                 GCACCCGCUGUUCCAGCCCCUGCACACCGUGAUGCGGGAA 
                   
               
               
                   
                 ACCCUGUUCAUCGGAAGCCACGUCGUGCUGCGGGAGCUG 
                   
               
               
                   
                 AGGCUGAACGUAACCACCCAGGGCCCUAAUCUGGCCCUG 
                   
               
               
                   
                 UAUCAGCUCCUCAGUACCGCCCUGUGCAGCGCCCUUGAGA 
                   
               
               
                   
                 UCGGCGAGGUGCUCAGAGGCCUGGCCCUCGGUACCGAGA 
                   
               
               
                   
                 GCGGCCUCUUCAGCCCAUGCUACUUAAGCCUGCGGUUCG 
                   
               
               
                   
                 ACCUGACCCGGGACAAGUUGCUGAGCAUGGCCCCGCAGG 
                   
               
               
                   
                 AGGCCACACUGGACCAGGCAGCUGUAUCCAACGCCGUGG 
                   
               
               
                   
                 ACGGCUUCCUGGGCAGACUGUCCCUGGAACGGGAGGACC 
                   
               
               
                   
                 GGGACGCCUGGCACCUGCCUGCCUACAAGUGUGUGGAUC 
                   
               
               
                   
                 GGCUGGACAAGGUGCUGAUGAUCAUCCCUCUGAUUAAUG 
                   
               
               
                   
                 UCACCUUCAUCAUCAGCAGCGACCGGGAGGUGCGGGGAU 
                   
               
               
                   
                 CCGCCCUCUACGAGGCCAGCACCACCUAUCUGAGCAGCAG 
                   
               
               
                   
                 CCUGUUCCUGUCUCCUGUGAUCAUGAACAAGUGCAGCCA 
                   
               
               
                   
                 GGGCGCCGUGGCCGGCGAGCCCCGGCAGAUCCCCAAGAUC 
                   
               
               
                   
                 CAGAACUUCACCCGGACCCAGAAGUCUUGCAUCUUCUGC 
                   
               
               
                   
                 GGCUUCGCCCUUUUGUCCUACGACGAGAAGGAGGGCUUG 
                   
               
               
                   
                 GAGACUACAACCUACAUCACCAGCCAGGAGGUGCAGAAC 
                   
               
               
                   
                 AGCAUCCUGUCAUCUAAUUACUUCGACUUCGACAACCUG 
                   
               
               
                   
                 CACGUUCAUUACCUGCUCCUCACCACCAACGGUACCGUCA 
                   
               
               
                   
                 UGGAAAUCGCCGGACUGUACGAGGAGCGGGCCCAUGUUG 
                   
               
               
                   
                 UGCUGGCCAUCAUCCUGUACUUCAUCGCUUUCGCACUUG 
                   
               
               
                   
                 GCAUCUUCCUGGUGCACAAGAUCGUGAUGUUCUUCCUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCC 
                 106 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MQLLCVFCLVLLWEVGAASLSEVKLHLDIEGHASHYTIPWTE 
                  38 
               
               
                 amino acid 
                 LMAKVPGLSPEALWREANVTEDLASMLNRYKLIYKTSGTLGI 
                   
               
               
                 sequence 
                 ALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSPACMLSAPLE 
                   
               
               
                   
                 KQLFYYIGTMLPNTRPHSYVFYQLRCHLSYVALSINGDKFQYT 
                   
               
               
                   
                 GAMTSKFLMGTYKRVTEKGDEHVLSLVFGKTKDLPDLRGPFS 
                   
               
               
                   
                 YPSLTSAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMFSRAV 
                   
               
               
                   
                 TMTAASYARYVLQKLVLLEMKGGCREPELDTETLTTMFEVS 
                   
               
               
                   
                 VAFFKVGHAVGETGNGCVDLRWLAKSFFELTVLKDIIGICYG 
                   
               
               
                   
                 ATVKGMQSYGLERLAAMLMATVKMEELGHLTTEKQEYALR 
                   
               
               
                   
                 LATVGYPKAGVYSGLIGGATSVLLSAYNRHPLFQPLHTVMRE 
                   
               
               
                   
                 TLFIGSHVVLRELRLNVTTQGPNLALYQLLSTALCSALEIGEVL 
                   
               
               
                   
                 RGLALGTESGLFSPCYLSLRFDLTRDKLLSMAPQEATLDQAA 
                   
               
               
                   
                 VSNAVDGFLGRLSLEREDRDAWHLPAYKCVDRLDKVLMIIPL 
                   
               
               
                   
                 INVTFIISSDREVRGSALYEASTTYLSSSLFLSPVIMNKCSQGAV 
                   
               
               
                   
                 AGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKEGLETTTYITSQ 
                   
               
               
                   
                 EVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEIAGLYEERAH 
                   
               
               
                   
                 VVLAIILYFIAFALGIFLVHKIVMFFL 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gL (B95-8) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 202 consists of from 5′ end to 3′ end: 
                 202 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 107, and 
                   
               
               
                 3′ UTR SEQ ID NO: 106 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGAGCCGUGGGCGUGUUCCUGGCCAUCUGCCUGGUG 
                 107 
               
               
                 Construct 
                 ACCAUCUUCGUGCUGCCCACCUGGGGCAACUGGGCCUACC 
                   
               
               
                 (excluding 
                 CCUGCUGCCACGUGACCCAGCUGAGAGCCCAGCACCUGCU 
                   
               
               
                 the stop 
                 GGCCCUGGAGAACAUCAGCGACAUCUACCUGGUGAGCAA 
                   
               
               
                 codon) 
                 CCAGACCUGCGACGGCUUCAGCCUGGCCAGCCUGAACAGC 
                   
               
               
                   
                 CCCAAGAACGGCAGCAACCAGCUGGUGAUCAGCAGAUGC 
                   
               
               
                   
                 GCCAACGGCCUGAACGUGGUGAGCUUCUUCAUCAGCAUC 
                   
               
               
                   
                 CUGAAGAGAAGCAGCAGCGCCCUGACCGGCCACCUGAGA 
                   
               
               
                   
                 GAGCUGCUGACCACCCUGGAGACCCUGUACGGCAGCUUC 
                   
               
               
                   
                 AGCGUGGAGGACCUGUUCGGCGCCAACCUGAACAGAUAC 
                   
               
               
                   
                 GCCUGGCACAGAGGCGGC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCC 
                 106 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MRAVGVFLAICLVTIFVLPTWGNWAYPCCHVTQLRAQHLLA 
                  36 
               
               
                 amino acid 
                 LENISDIYLVSNQTCDGFSLASLNSPKNGSNQLVISRCANGLNV 
                   
               
               
                 sequence 
                 VSFFISILKRSSSALTGHLRELLTTLETLYGSFSVEDLFGANLNR 
                   
               
               
                   
                 YAWHRGG 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp42 (B95-8) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 203 consists of from 5′ end to 3′ end: 
                 203 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 108, and 
                   
               
               
                 3′ UTR SEQ ID NO: 1-6 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGUGAGCUUCAAGCAGGUGAGAGUGCCCCUGUUCACC 
                 108 
               
               
                 Construct 
                 GCCAUCGCCCUGGUGAUCGUGCUGCUGCUGGCCUACUUCC 
                   
               
               
                 (excluding 
                 UGCCACCCAGAGUGAGAGGCGGCGGCAGAGUGGCCGCCG 
                   
               
               
                 the stop 
                 CCGCCAUCACCUGGGUGCCCAAGCCCAACGUGGAGGUGU 
                   
               
               
                 codon) 
                 GGCCCGUGGACCCGCCACCUCCCGUGAACUUCAACAAGAC 
                   
               
               
                   
                 CGCCGAGCAGGAGUACGGCGACAAGGAGGUGAAGCUGCC 
                   
               
               
                   
                 CCACUGGACGCCGACCCUGCACACCUUCCAGGUGCCCCAG 
                   
               
               
                   
                 AACUACACCAAGGCCAACUGCACCUACUGCAACACCAGAG 
                   
               
               
                   
                 AGUACACCUUCAGCUACAAGGGCUGCUGCUUCUACUUCA 
                   
               
               
                   
                 CCAAGAAGAAGCACACCUGGAACGGCUGCUUCCAGGCCU 
                   
               
               
                   
                 GCGCCGAGCUGUACCCCUGCACCUACUUCUACGGCCCCAC 
                   
               
               
                   
                 CCCAGACAUCCUGCCCGUGGUGACCAGAAACCUGAACGCC 
                   
               
               
                   
                 AUCGAGAGCCUGUGGGUGGGCGUGUACAGAGUGGGCGAG 
                   
               
               
                   
                 GGCAACUGGACCAGCCUGGACGGCGGCACCUUCAAGGUG 
                   
               
               
                   
                 UACCAGAUCUUCGGCAGCCACUGCACCUACGUGAGCAAG 
                   
               
               
                   
                 UUCAGCACCGUGCCCGUGAGCCACCACGAGUGCAGCUUCC 
                   
               
               
                   
                 UGAAGCCCUGCCUGUGCGUGAGCCAGAGAAGCAACAGC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCC 
                 106 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MVSFKQVRVPLFTAIALVIVLLLAYFLPPRVRGGGRVAAAAIT 
                  34 
               
               
                 amino acid 
                 WVPKPNVEVWPVDPPPPVNFNKTAEQEYGDKEVKLPHWTPT 
                   
               
               
                 sequence 
                 LHTFQVPQNYTKANCTYCNTREYTFSYKGCCFYFTKKKHTW 
                   
               
               
                   
                 NGCFQACAELYPCTYFYGPTPDILPVVTRNLNAIESLWVGVYR 
                   
               
               
                   
                 VGEGNWTSLDGGTFKVYQIFGSHCTYVSKFSTVPVSHHECSFL 
                   
               
               
                   
                 KPCLCVSQRSNS 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350_001 (modified) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 204 consists of from 5′ end to 3′ end: 
                 204 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 109, and 
                   
               
               
                 3′ UTR SEQ ID NO: 106 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                 109 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUACAAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAACUAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 UCUGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCACUUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUACUGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCACGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCC 
                 106 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  28 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV D123_Ferritin_RX 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 205 consists of from 5′ end to 3′ end: 
                 205 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 110, and 
                   
               
               
                 3′ UTR SEQ ID NO: 106 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGACAGCAAGGGCAGCAGCCAGAAGGGAUCGAGGCUA 
                 110 
               
               
                 Construct 
                 UUAUUGUUACUUGUGGUGAGCAACUUGCUCCUGCCUCAG 
                   
               
               
                 (excluding 
                 GGCGUGGUGGGCGAGGCCGCCUUGUUGGUGUGCCAGUAC 
                   
               
               
                 the stop 
                 ACCAUCCAGAGCCUCAUCCACCUCACCGGCGAGGACCCCG 
                   
               
               
                 codon) 
                 GCUUCUUCAACGUGGAGAUCCCCGAGUUCCCCUUCUACCC 
                   
               
               
                   
                 CACCUGCAACGUGUGCACCGCCGACGUGAACGUGACCAUC 
                   
               
               
                   
                 AACUUCGACGUGGGCGGCAAGAAGCACCAGCUGGACCUG 
                   
               
               
                   
                 GACUUCGGCCAGCUGACCCCGCACACCAAGGCCGUGUACC 
                   
               
               
                   
                 AGCCCAGGGGCGCCUUCGGCGGCAGCGAGAACGCCACCAA 
                   
               
               
                   
                 CCUGUUCCUGCUGGAGCUGCUGGGCGCCGGCGAGCUGGC 
                   
               
               
                   
                 CCUGACCAUGAGGUCCAAGAAGCUGCCCAUCAAUGUGAC 
                   
               
               
                   
                 CACAGGAGAGGAGCAGCAGGUGAGCCUGGAGAGCGUGGA 
                   
               
               
                   
                 CGUGUACUUCCAGGACGUGUUCGGCACCAUGUGGUGCCA 
                   
               
               
                   
                 CCACGCCGAGAUGCAGAACCCAGUUUACUUGAUUCCUGA 
                   
               
               
                   
                 GACAGUGCCCUACAUCAAGUGGGACAACUGCAACAGCAC 
                   
               
               
                   
                 CAACAUCACCGCCGUGGUGAGGGCCCAGGGCCUGGACGU 
                   
               
               
                   
                 GACCCUGCCCCUGAGCCUGCCCACCAGCGCCCAGGAUAGC 
                   
               
               
                   
                 AACUUCAGCGUGAAGACCGAAAUGCUGGGCAACGAGAUC 
                   
               
               
                   
                 GACAUCGAGUGCAUCAUGGAGGACGGCGAGAUCAGCCAG 
                   
               
               
                   
                 GUGCUGCCCGGCGACAACAAGUUCAACAUUACCUGCAGC 
                   
               
               
                   
                 GGCUACGAGAGCCACGUGCCCAGCGGCGGCAUCCUGACCU 
                   
               
               
                   
                 CAACUUCACCCGUGGCCACUCCGAUCCCCGGCACCGGCUA 
                   
               
               
                   
                 CGCCUACAGCCUGCGUCUGACACCUAGGCCCGUGAGCAGG 
                   
               
               
                   
                 UUCCUGGGCAAUAACAGCAUCCUGUACGUGUUCUACAGC 
                   
               
               
                   
                 GGCAACGGCCCCAAGGCUUCCGGCGGCGACUACUGUAUU 
                   
               
               
                   
                 CAAUCAAACAUCGUGUUCAGCGACGAGAUACCAGCCAGC 
                   
               
               
                   
                 CAGGACAUGCCUACUAACACCACCGACAUCACCUAUGUG 
                   
               
               
                   
                 GGAGACAAUGCUACUUACAGCGUGCCCAUGGUGACCAGC 
                   
               
               
                   
                 GAGGACGCCAACAGCCCAAAUGUCACCGUGACCGCCUUCU 
                   
               
               
                   
                 GGGCCUGGCCCAACAACACUGAGACAGACUUCAAGUGCA 
                   
               
               
                   
                 AGUGGACCCUCACAUCCGGCACCCCUAGCGGAUGCGAGA 
                   
               
               
                   
                 ACAUCAGCGGAGCGUUCGCCAGCAACAGGACCUUCGAUA 
                   
               
               
                   
                 UAACAGUGAGCGGCCUGGGCACCGCUCCUAAGACCCUGA 
                   
               
               
                   
                 UCAUCACCAGGACGGCCACAAAUGCAACUACUACUACAC 
                   
               
               
                   
                 ACAAGGUGAUCUUCAGCAAGGCUCCGGAAUCACAAGUGA 
                   
               
               
                   
                 GGCAGCAGUUCUCAAAGGAUAUCGAGAAGCUUCUGAACG 
                   
               
               
                   
                 AGCAAGUUAACAAGGAAAUGCAGAGCAGUAAUCUCUACA 
                   
               
               
                   
                 UGAGCAUGAGCAGCUGGUGCUACACCCACUCCCUGGACG 
                   
               
               
                   
                 GAGCAGGCCUCUUCCUGUUCGACCACGCAGCCGAGGAGU 
                   
               
               
                   
                 ACGAGCACGCUAAGAAGUUGAUCAUUUUCUUGAACGAGA 
                   
               
               
                   
                 ACAACGUGCCCGUGCAGCUAACGUCAAUCAGCGCACCUG 
                   
               
               
                   
                 AGCACAAGUUCGAGGGCCUGACCCAGAUCUUCCAGAAGG 
                   
               
               
                   
                 CCUACGAACACGAACAGCACAUCUCCGAGAGCAUCAACA 
                   
               
               
                   
                 AUAUUGUGGAUCACGCUAUCAAGUCCAAGGACCACGCUA 
                   
               
               
                   
                 CCUUCAACUUCCUGCAGUGGUACGUGGCCGAGCAACAUG 
                   
               
               
                   
                 AGGAGGAGGUGCUGUUCAAGGACAUCCUGGACAAGAUCG 
                   
               
               
                   
                 AGCUGAUCGGUAAUGAGAAUCACGGCCUGUACCUGGCCG 
                   
               
               
                   
                 ACCAGUACGUGAAGGGCAUCGCCAAGAGCCGGAAGUCA 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCC 
                 106 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MDSKGSSQKGSRLLLLLVVSNLLLPQGVVGEAALLVCQYTIQ 
                 111 
               
               
                 amino acid 
                 SLIHLTGEDPGFFNVEIPEFPFYPTCNVCTADVNVTINFDVGGK 
                   
               
               
                 sequence 
                 KHQLDLDFGQLTPHTKAVYQPRGAFGGSENATNLFLLELLGA 
                   
               
               
                   
                 GELALTMRSKKLPINVTTGEEQQVSLESVDVYFQDVFGTMWC 
                   
               
               
                   
                 HHAEMQNPVYLIPETVPYIKWDNCNSTNITAVVRAQGLDVTL 
                   
               
               
                   
                 PLSLPTSAQDSNFSVKTEMLGNEIDIECIMEDGEISQVLPGDNK 
                   
               
               
                   
                 FNITCSGYESHVPSGGILTSTSPVATPIPGTGYAYSLRLTPRPVS 
                   
               
               
                   
                 RFLGNNSILYVFYSGNGPKASGGDYCIQSNIVFSDEIPASQDMP 
                   
               
               
                   
                 TNTTDITYVGDNATYSVPMVTSEDANSPNVTVTAFWAWPNN 
                   
               
               
                   
                 TETDFKCKWTLTSGTPSGCENISGAFASNRTFDITVSGLGTAPK 
                   
               
               
                   
                 TLIITRTATNATTTTHKVIFSKAPESQVRQQFSKDIEKLLNEQV 
                   
               
               
                   
                 NKEMQSSNLYMSMSSWCYTHSLDGAGLFLFDHAAEEYEHAK 
                   
               
               
                   
                 KLIIFLNENNVPVQLTSISAPEHKFEGLTQIFQKAYEHEQHISESI 
                   
               
               
                   
                 NNIVDHAIKSKDHATFNFLQWYVAEQHEEEVLFKDILDKIELI 
                   
               
               
                   
                 GNENHGLYLADQYVKGIAKSRKS 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV D123_LS 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 206 consists of from 5′ end to 3′ end: 
                 206 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 112, and 
                   
               
               
                 3′ UTR SEQ ID NO: 106 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGACAGCAAGGGCAGCAGCCAGAAGGGCUCCCGGUUG 
                 112 
               
               
                 Construct 
                 UUGCUCUUGCUCGUGGUGAGCAACUUGUUGUUGCCUCAG 
                   
               
               
                 (excluding 
                 GGCGUGGUGGGCGAGGCCGCCUUGUUGGUGUGCCAGUAC 
                   
               
               
                 the stop 
                 ACCAUCCAGAGCCUCAUCCACCUCACCGGCGAGGACCCUG 
                   
               
               
                 codon) 
                 GCUUCUUCAACGUGGAGAUCCCUGAGUUCCCUUUCUACC 
                   
               
               
                   
                 CUACCUGCAACGUGUGCACCGCCGACGUGAACGUGACCA 
                   
               
               
                   
                 UCAACUUCGACGUGGGCGGCAAGAAGCACCAGCUGGACC 
                   
               
               
                   
                 UGGACUUCGGCCAGCUGACCCCUCACACCAAGGCCGUGUA 
                   
               
               
                   
                 CCAGCCUAGAGGCGCCUUCGGCGGCAGCGAGAACGCCACC 
                   
               
               
                   
                 AACCUGUUCCUGCUGGAGCUGCUGGGCGCCGGCGAGCUG 
                   
               
               
                   
                 GCCCUGACCAUGAGAAGUAAGAAGCUGCCUAUCAACGUC 
                   
               
               
                   
                 ACUACUGGCGAGGAGCAGCAGGUGAGCCUGGAGAGCGUG 
                   
               
               
                   
                 GACGUGUACUUCCAGGACGUGUUCGGCACCAUGUGGUGC 
                   
               
               
                   
                 CACCACGCCGAGAUGCAGAACCCUGUGUACCUGAUCCCAG 
                   
               
               
                   
                 AAACCGUGCCUUACAUCAAGUGGGACAACUGCAACAGCA 
                   
               
               
                   
                 CCAACAUCACCGCUGUGGUGAGAGCCCAGGGCCUGGACG 
                   
               
               
                   
                 UGACCCUGCCUCUGAGCCUGCCUACCAGCGCCCAAGACAG 
                   
               
               
                   
                 UAACUUCAGCGUGAAGACGGAGAUGCUGGGCAACGAGAU 
                   
               
               
                   
                 CGACAUCGAGUGCAUCAUGGAGGACGGCGAGAUCAGCCA 
                   
               
               
                   
                 GGUGCUGCCUGGCGACAACAAGUUCAACAUAACAUGCAG 
                   
               
               
                   
                 CGGCUACGAGAGCCACGUGCCUAGCGGCGGCAUCCUGACC 
                   
               
               
                   
                 UCCACCAGCCCUGUGGCCACCCCUAUCCCUGGCACCGGCU 
                   
               
               
                   
                 ACGCCUACAGCCUGAGACUCACGCCAAGACCUGUGAGCA 
                   
               
               
                   
                 GAUUCUUAGGAAACAACAGCAUCCUGUACGUGUUCUACA 
                   
               
               
                   
                 GCGGCAACGGCCCUAAGGCCUCCGGCGGUGACUACUGUA 
                   
               
               
                   
                 UCCAGUCUAACAUCGUGUUCAGCGACGAAAUCCCAGCCA 
                   
               
               
                   
                 GCCAGGACAUGCCUACAAACACCACCGACAUCACUUAUG 
                   
               
               
                   
                 UCGGCGACAAUGCGACAUACAGCGUGCCUAUGGUGACCA 
                   
               
               
                   
                 GCGAGGACGCCAACAGCCCUAACGUAACCGUGACCGCCUU 
                   
               
               
                   
                 CUGGGCCUGGCCUAACAACACCGAAACCGACUUCAAGUG 
                   
               
               
                   
                 CAAGUGGACUUUAACUAGUGGCACCCCUUCUGGCUGCGA 
                   
               
               
                   
                 GAACAUCAGCGGCGCAUUCGCCAGCAACAGAACCUUCGA 
                   
               
               
                   
                 UAUCACCGUGAGCGGCCUGGGCACCGCCCCUAAGACCCUG 
                   
               
               
                   
                 AUCAUCACCAGAACCGCAACUAAUGCAACUACAACAACCC 
                   
               
               
                   
                 ACAAGGUGAUCUUCAGCAAGGCCCCUGGCUCCGGCUCUG 
                   
               
               
                   
                 CCGGCAGUGGCAGCGCAGGCAGUGGAUCCGCCGGUUCAG 
                   
               
               
                   
                 GUUCCGCCAUGCAGAUCUACGAGGGCAAGCUGACCGCCG 
                   
               
               
                   
                 AGGGCCUGAGAUUCGGCAUCGUGGCCAGCAGGUUCAACC 
                   
               
               
                   
                 ACGCCCUGGUGGACAGACUGGUGGAGGGCGCCAUCGACG 
                   
               
               
                   
                 CCAUCGUGAGACACGGAGGAAGAGAGGAGGAUAUAACAC 
                   
               
               
                   
                 UGGUCAGAGUGCCUGGAUCAUGGGAAAUCCCUGUCGCCG 
                   
               
               
                   
                 CUGGUGAGCUGGCAAGAAAGGAGGACAUCGAUGCGGUGA 
                   
               
               
                   
                 UCGCCAUCGGCGUGCUGAUCCGCGGUGCAACUCCACACUU 
                   
               
               
                   
                 CGACUACAUCGCCUCUGAAGUGUCAAAGGGCCUUGCGGA 
                   
               
               
                   
                 CCUGUCCCUCGAACUCAGAAAGCCUAUCACAUUCGGAGU 
                   
               
               
                   
                 GAUUACUGCAGAUACCCUGGAGCAGGCCAUCGAGAGAGC 
                   
               
               
                   
                 CGGCACCAAGCACGGCAACAAGGGCUGGGAAGCCGCGCU 
                   
               
               
                   
                 GUCUGCCAUAGAAAUGGCAAACCUUUUCAAGUCCCUUAG 
                   
               
               
                   
                 A 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCC 
                 106 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MDSKGSSQKGSRLLLLLVVSNLLLPQGVVGEAALLVCQYTIQ 
                 113 
               
               
                 amino acid 
                 SLIHLTGEDPGFFNVEIPEFPFYPTCNVCTADVNVTINFDVGGK 
                   
               
               
                 sequence 
                 KHQLDLDFGQLTPHTKAVYQPRGAFGGSENATNLFLLELLGA 
                   
               
               
                   
                 GELALTMRSKKLPINVTTGEEQQVSLESVDVYFQDVFGTMWC 
                   
               
               
                   
                 HHAEMQNPVYLIPETVPYIKWDNCNSTNITAVVRAQGLDVTL 
                   
               
               
                   
                 PLSLPTSAQDSNFSVKTEMLGNEIDIECIMEDGEISQVLPGDNK 
                   
               
               
                   
                 FNITCSGYESHVPSGGILTSTSPVATPIPGTGYAYSLRLTPRPVS 
                   
               
               
                   
                 RFLGNNSILYVFYSGNGPKASGGDYCIQSNIVFSDEIPASQDMP 
                   
               
               
                   
                 TNTTDITYVGDNATYSVPMVTSEDANSPNVTVTAFWAWPNN 
                   
               
               
                   
                 TETDFKCKWTLTSGTPSGCENISGAFASNRTFDITVSGLGTAPK 
                   
               
               
                   
                 TLIITRTATNATTTTHKVIFSKAPGSGSAGSGSAGSGSAGSGSA 
                   
               
               
                   
                 MQIYEGKLTAEGLRFGIVASRFNHALVDRLVEGAIDAIVRHGG 
                   
               
               
                   
                 REEDITLVRVPGSWEIPVAAGELARKEDIDAVIAIGVLIRGATP 
                   
               
               
                   
                 HFDYIASEVSKGLADLSLELRKPITFGVITADTLEQAIERAGTK 
                   
               
               
                   
                 HGNKGWEAALSAIEMANLFKSLR 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350_004 (modified) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 207 consists of from 5′ end to 3′ end: 
                 207 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 124, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAAGCCGCCCUAUUAGUCUGCCAGUACACCAUUCAA 
                 124 
               
               
                 Construct 
                 AGCCUCAUCCACUUGACUGGGGAGGAUCCUGGGUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCGGAAUUCCCUUUCUACCCAACUUGC 
                   
               
               
                 the stop 
                 AACGUGUGCACGGCUGACGUCAACGUGACUAUCAACUUC 
                   
               
               
                 codon) 
                 GAUGUGGGAGGGAAGAAACAUCAACUUGAUCUGGACUUU 
                   
               
               
                   
                 GGCCAGCUUACUCCCCACACCAAGGCCGUGUACCAGCCCC 
                   
               
               
                   
                 GCGGCGCAUUCGGUGGGAGCGAGAACGCGACAAAUCUAU 
                   
               
               
                   
                 UCUUGCUGGAACUGCUGGGCGCAGGAGAGCUCGCCCUGA 
                   
               
               
                   
                 CGAUGCGAUCUAAGAAGCUUCCCAUCAACGUUACCACUG 
                   
               
               
                   
                 GCGAGGAGCAGCAGGUGUCCCUCGAAAGCGUCGACGUCU 
                   
               
               
                   
                 AUUUCCAAGACGUCUUCGGAACAAUGUGGUGCCACCACG 
                   
               
               
                   
                 CUGAGAUGCAGAACCCCGUAUAUCUUAUUCCCGAAACCG 
                   
               
               
                   
                 UGCCAUACAUCAAGUGGGACAACUGCAACUCAACUAACA 
                   
               
               
                   
                 UCACUGCAGUAGUGCGCGCCCAGGGACUGGACGUCACUU 
                   
               
               
                   
                 UGCCACUGUCACUGCCCACAUCAGCCCAAGACAGCAACUU 
                   
               
               
                   
                 CAGCGUCAAGACAGAAAUGCUGGGCAACGAAAUCGACAU 
                   
               
               
                   
                 CGAAUGCAUAAUGGAGGAUGGGGAAAUUUCCCAGGUCCU 
                   
               
               
                   
                 CCCCGGUGAUAACAAGUUUAAUAUUACUUGUUCUGGAUA 
                   
               
               
                   
                 UGAAUCCCAUGUGCCGUCUGGUGGUAUACUGACGAGUAC 
                   
               
               
                   
                 AUCGCCAGUGGCUACCCCAAUCCCCGGGACAGGCUAUGCC 
                   
               
               
                   
                 UACAGCCUGAGACUGACGCCAAGACCAGUUUCCAGGUUU 
                   
               
               
                   
                 CUGGGGAAUAACUCUAUCCUGUAUGUGUUUUAUUCUGGU 
                   
               
               
                   
                 AACGGACCCAAGGCAUCCGGCGGUGAUUAUUGUAUCCAG 
                   
               
               
                   
                 AGCAAUAUAGUGUUCUCUGAUGAGAUUCCGGCCUCCCAG 
                   
               
               
                   
                 GAUAUGCCAACCAACACGACAGAUAUCACCUAUGUCGGG 
                   
               
               
                   
                 GACAACGCCACAUAUAGCGUGCCAAUGGUGACCAGCGAA 
                   
               
               
                   
                 GAUGCCAAUUCUCCCAACGUCACAGUGACAGCCUUUUGG 
                   
               
               
                   
                 GCGUGGCCUAAUAACACAGAAACUGACUUUAAAUGCAAA 
                   
               
               
                   
                 UGGACUCUAACCUCAGGCACCCCAUCUGGCUGCGAGAAC 
                   
               
               
                   
                 AUAAGCGGCGCGUUUGCAUCGAACAGAACUUUUGAUAUU 
                   
               
               
                   
                 ACAGUAUCUGGGUUGGGCACUGCGCCCAAGACUCUAAUA 
                   
               
               
                   
                 AUUACACGGACGGCCACUAACGCCACCACGACGACUCACA 
                   
               
               
                   
                 AGGUGAUCUUCAGCAAAGCUCCCGAAUCUACUACUACCU 
                   
               
               
                   
                 CGCCGACACUGAAUACGACAGGAUUCGCCGACCCAAAUA 
                   
               
               
                   
                 CUACGACCGGCCUUCCCAGCUCGACACAUGUGCCUACAAA 
                   
               
               
                   
                 CUUAACGGCGCCCGCCAGUACCGGCCCCACCGUGAGCACC 
                   
               
               
                   
                 GCUGACGUCACCUCUCCUACCCCUGCCGGGACUACAAGUG 
                   
               
               
                   
                 GUGCUUCCCCAGUUACUCCCAGCCCUAGCCCUUGGGACAA 
                   
               
               
                   
                 CGGCACUGAGAGCAAAGCUCCGGACAUGACCAGCAGUAC 
                   
               
               
                   
                 CAGUCCAGUGACAACUCCGACACCCAAUGCGACGUCCCCA 
                   
               
               
                   
                 ACUCCCGCCGUGACCACGCCAACACCAAAUGCCACGUCAC 
                   
               
               
                   
                 CUACUCCAGCGGUCACCACCCCAACGCCAAACGCUACUAG 
                   
               
               
                   
                 UCCCACUCUAGGCAAGACAUCUCCCACCUCCGCUGUCACC 
                   
               
               
                   
                 ACCCCUACACCCAAUGCUACCGGUCCCACGGUGGGUGAGA 
                   
               
               
                   
                 CUAGCCCACAGGCCAACGCAACUAAUCAUACAUUGGGUG 
                   
               
               
                   
                 GCACAAGCCCAACGCCCGUCGUCACUAGCCAGCCUAAGAA 
                   
               
               
                   
                 CGCCACAAGCGCUGUAACUACCGGCCAGCACAAUAUAAC 
                   
               
               
                   
                 AAGUUCCAGUACUAGCAGUAUGUCUCUGAGACCCAGCUC 
                   
               
               
                   
                 AAAUCCUGAAACCCUCUCGCCCUCUACGUCGGAUAACUCC 
                   
               
               
                   
                 ACGUCCCACAUGCCUCUACUCACAAGCGCGCACCCUACCG 
                   
               
               
                   
                 GAGGAGAGAAUAUCACUCAGGUUACCCCAGCUUCUAUCU 
                   
               
               
                   
                 CGACUCACCAUGUCUCUACUUCUUCCCCAGCGCCACGGCC 
                   
               
               
                   
                 CGGCACUACCAGCCAGGCUAGCGGGCCAGGUAAUUCCUCC 
                   
               
               
                   
                 ACUAGCACUAAACCUGGCGAAGUCAACGUUACCAAGGGA 
                   
               
               
                   
                 ACACCUCCCCAGAAUGCAACCUCGCCCCAAGCUCCCUCUG 
                   
               
               
                   
                 GACAGAAGACAGCCGUCCCUACCGUCACAAGCACCGGUG 
                   
               
               
                   
                 GGAAGGCGAAUUCUACGACAGGCGGGAAGCACACUACCG 
                   
               
               
                   
                 GACACGGAGCAAGGACAUCCACUGAGCCCACGACCGACU 
                   
               
               
                   
                 ACGGUGGCGACUCCACCACGCCCAGACCCAGGUACAAUGC 
                   
               
               
                   
                 GACUACCUACCUGCCGCCUUCCACUUCCAGCAAACUGAGG 
                   
               
               
                   
                 CCUCGUUGGACUUUCACUUCUCCACCAGUCACCACCGCUC 
                   
               
               
                   
                 AGGCUACCGUGCCUGUCCCGCCGACCUCUCAGCCCCGGUU 
                   
               
               
                   
                 UUCAAAUUUGAGUAUGCUCGUGCUGCAGUGGGCCAGCCU 
                   
               
               
                   
                 GGCAGUGCUUACCCUCCUGCUCUUGCUGGUUAUGGCUGA 
                   
               
               
                   
                 CUGCGCCUUCCGACGUAACUUAUCCACCAGUCAUACUUAC 
                   
               
               
                   
                 ACUACACCUCCUUAUGACGACGCGGAGACCUACGUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                 125 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gp350_005 (modified) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 208 consists of from 5′ end to 3′ end: 
                 208 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 126, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAAGCCGCCUUACUGGUCUGCCAGUACACUAUUCAG 
                 126 
               
               
                 Construct 
                 UCCCUCAUCCAUCUCACAGGCGAGGACCCGGGGUUCUUCA 
                   
               
               
                 (excluding 
                 ACGUCGAAAUCCCCGAAUUCCCAUUCUAUCCCACCUGUAA 
                   
               
               
                 the stop 
                 CGUCUGCACAGCCGACGUUAACGUUACAAUCAAUUUCGA 
                   
               
               
                 codon) 
                 CGUGGGCGGGAAGAAACACCAGCUGGACCUCGACUUUGG 
                   
               
               
                   
                 GCAGCUGACGCCCCACACUAAAGCCGUUUACCAGCCCAGG 
                   
               
               
                   
                 GGUGCAUUCGGUGGAUCUGAGAAUGCUACAAACCUCUUU 
                   
               
               
                   
                 CUGCUUGAGCUGCUUGGUGCUGGAGAGCUGGCCCUGACA 
                   
               
               
                   
                 AUGCGCAGCAAGAAGUUGCCAAUCAAUGUGACCACAGGU 
                   
               
               
                   
                 GAAGAACAGCAGGUGUCCCUAGAAUCCGUGGAUGUGUAC 
                   
               
               
                   
                 UUCCAGGACGUUUUCGGCACAAUGUGGUGCCAUCACGCA 
                   
               
               
                   
                 GAGAUGCAGAAUCCGGUGUACCUUAUCCCCGAAACAGUG 
                   
               
               
                   
                 CCGUACAUUAAAUGGGAUAAUUGUAACUCCACAAACAUC 
                   
               
               
                   
                 ACGGCAGUGGUGCGCGCUCAGGGGCUGGACGUGACACUC 
                   
               
               
                   
                 CCGCUUUCCUUACCAACAAGUGCUCAAGAUUCCAACUUU 
                   
               
               
                   
                 UCUGUGAAGACCGAGAUGCUGGGCAACGAGAUUGAUAUU 
                   
               
               
                   
                 GAAUGCAUCAUGGAGGAUGGCGAGAUUAGCCAGGUGUUG 
                   
               
               
                   
                 CCCGGCGACAAUAAAUUUAAUAUUACCUGUUCCGGCUAU 
                   
               
               
                   
                 GAGUCACACGUGCCAAGUGGAGGCAUUCUCACGAGCACC 
                   
               
               
                   
                 UCUCCCGUUGCAACACCCAUUCCUGGGACAGGGUACGCA 
                   
               
               
                   
                 UAUAGUCUCAGGCUGACACCGAGACCGGUAUCGCGCUUC 
                   
               
               
                   
                 UUAGGUAACAAUUCCAUCUUGUAUGUGUUUUACAGCGGA 
                   
               
               
                   
                 AACGGGCCAAAGGCCUCAGGAGGAGACUACUGCAUCCAG 
                   
               
               
                   
                 AGCAAUAUAGUCUUUAGCGACGAGAUCCCUGCCUCACAA 
                   
               
               
                   
                 GACAUGCCCACCAACACCACCGACAUCACGUACGUCGGUG 
                   
               
               
                   
                 AUAAUGCCACUUACAGCGUGCCUAUGGUGACCUCUGAAG 
                   
               
               
                   
                 ACGCAAAUUCACCCAAUGUCACGGUUACCGCAUUCUGGG 
                   
               
               
                   
                 CUUGGCCAAAUAACACUGAAACCGACUUCAAGUGUAAAU 
                   
               
               
                   
                 GGACCCUAACCAGCGGCACCCCAUCAGGGUGCGAGAAUA 
                   
               
               
                   
                 UCAGCGGGGCCUUCGCCAGCAACCGGACAUUCGACAUCAC 
                   
               
               
                   
                 CGUGUCAGGACUAGGGACUGCUCCAAAGACAUUAAUUAU 
                   
               
               
                   
                 UACACGGACAGCAACUAACGCUACGACUACGACUCACAA 
                   
               
               
                   
                 AGUGAUAUUUAGCAAAGCGCCCGAAUCGACUACUACGAG 
                   
               
               
                   
                 CCCGACCCUAAACACCACCGGUUUCGCCGAUCCCAACACA 
                   
               
               
                   
                 ACUACUGGUCUCCCAUCUUCGACCCAUGUUCCAACGAAUC 
                   
               
               
                   
                 UGACAGCUCCAGCAAGUACGGGGCCCACUGUGAGUACAG 
                   
               
               
                   
                 CCGACGUUACUAGCCCAACUCCCGCGGGUACGACUAGUG 
                   
               
               
                   
                 GCGCGUCUCCAGUGACCCCUUCGCCUUCUCCGUGGGAUAA 
                   
               
               
                   
                 UGGAACCGAGUCAAAGGCUCCUGAUAUGACCAGCUCCAC 
                   
               
               
                   
                 CAGUCCCGUUACAACGCCAACGCCCAAUGCAACGAGUCCA 
                   
               
               
                   
                 ACUCCGGCGGUUACCACUCCGACACCAAACGCCACCUCGC 
                   
               
               
                   
                 CCACGCCUGCAGUUACUACGCCCACCCCUAAUGCUACGUC 
                   
               
               
                   
                 ACCCACUCUGGGAAAGACCUCACCAACCUCUGCCGUGACU 
                   
               
               
                   
                 ACUCCUACACCUAAUGCCACAGGACCCACAGUUGGGGAA 
                   
               
               
                   
                 ACCAGCCCACAGGCUAACGCAACUAAUCAUACGCUUGGC 
                   
               
               
                   
                 GGGACCUCUCCCACGCCCGUGGUCACUUCACAGCCUAAGA 
                   
               
               
                   
                 AUGCCACAAGCGCUGUGACUACAGGCCAGCAUAAUAUAA 
                   
               
               
                   
                 CCAGCAGUUCAACCAGUAGCAUGUCCCUGCGUCCUUCAUC 
                   
               
               
                   
                 CAAUCCUGAGACUCUGAGCCCCAGCACUAGUGACAACUC 
                   
               
               
                   
                 AACUUCUCACAUGCCGUUGCUGACCUCCGCGCAUCCCACG 
                   
               
               
                   
                 GGCGGUGAGAAUAUCACACAGGUGACACCCGCGUCUAUU 
                   
               
               
                   
                 AGCACACAUCACGUGAGUACGUCCUCGCCCGCACCCCGCC 
                   
               
               
                   
                 CAGGCACAACCUCACAAGCAUCAGGUCCGGGGAAUAGCA 
                   
               
               
                   
                 GCACAAGUACCAAGCCUGGCGAAGUGAAUGUAACCAAGG 
                   
               
               
                   
                 GCACGCCACCGCAGAACGCCACAUCCCCUCAAGCCCCAUC 
                   
               
               
                   
                 CGGCCAGAAGACCGCUGUACCCACCGUGACAUCAACUGG 
                   
               
               
                   
                 GGGGAAAGCGAACUCAACUACCGGUGGGAAGCAUACAAC 
                   
               
               
                   
                 AGGGCACGGUGCCAGAACUUCUACAGAGCCAACCACGGA 
                   
               
               
                   
                 CUACGGAGGGGACAGCACAACGCCCAGACCGCGGUACAA 
                   
               
               
                   
                 UGCGACCACAUACCUGCCUCCCUCAACUUCGAGUAAGCUG 
                   
               
               
                   
                 CGACCCAGAUGGACCUUUACUUCUCCUCCUGUCACCACCG 
                   
               
               
                   
                 CUCAAGCCACCGUCCCGGUUCCACCUACUUCCCAGCCUAG 
                   
               
               
                   
                 AUUCUCCAAUCUGAGCAUGCUUGUCCUACAGUGGGCAUC 
                   
               
               
                   
                 ACUCGCGGUACUAACCUUGCUGCUACUGUUGGUGAUGGC 
                   
               
               
                   
                 GGACUGCGCGUUUCGCCGCAACUUAUCCACCUCCCACACG 
                   
               
               
                   
                 UACACAACACCUCCCUAUGAUGACGCUGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                 127 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENATNLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSTN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFSVKTEMLGNEIDIECIM 
                   
               
               
                   
                 EDGEISQVLPGDNKFNITCSGYESHVPSGGILTSTSPVATPIPGT 
                   
               
               
                   
                 GYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCIQS 
                   
               
               
                   
                 NIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDANSP 
                   
               
               
                   
                 NVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASN 
                   
               
               
                   
                 RTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTS 
                   
               
               
                   
                 PTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADVTS 
                   
               
               
                   
                 PTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPTPN 
                   
               
               
                   
                 ATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSAVT 
                   
               
               
                   
                 TPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNATS 
                   
               
               
                   
                 AVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLLTS 
                   
               
               
                   
                 AHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGNSS 
                   
               
               
                   
                 TSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGKA 
                   
               
               
                   
                 NSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYLPP 
                   
               
               
                   
                 STSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVLQ 
                   
               
               
                   
                 WASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETYV 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 SE_EBV gB_019 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 209 consists of from 5′ end to 3′ end: 
                 209 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 128, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGACCCGGCGGCGGGUGUUAAGCGUGGUGGUGCUCUUA 
                 128 
               
               
                 Construct 
                 GCCGCCUUAGCCUGCCGGCUUGGCGCCCAGACUCCGGAGC 
                   
               
               
                 (excluding 
                 AGCCCGCUCCUCCCGCCACCACCGUCCAGCCCACCGCCAC 
                   
               
               
                 the stop 
                 CCGCCAGCAGACCUCCUUCCCCUUCCGCGUCUGCGAGCUC 
                   
               
               
                 codon) 
                 UCCUCCCACGGCGACCUCUUCCGCUUCUCCUCCGACAUCC 
                   
               
               
                   
                 AGUGCCCCUCCUUCGGCACCCGCGAGAACCACACCGAGGG 
                   
               
               
                   
                 CCUCCUCAUGGUCUUCAAGGACAACAUCAUCCCCUACUCC 
                   
               
               
                   
                 UUCAAGGUCCGCUCCUACACCAAGAUCGUCACCAACAUCC 
                   
               
               
                   
                 UCAUCUACAACGGCUGGUACGCCGACUCUGUGACUAAUC 
                   
               
               
                   
                 GCCACGAGGAGAAGUUCUCCGUCGACUCCUACGAGACUG 
                   
               
               
                   
                 ACCAGAUGGACACCAUCUACCAGUGCUACAACGCCGUCA 
                   
               
               
                   
                 AGAUGACCAAGGACGGCCUCACCCGCGUCUACGUCGACCG 
                   
               
               
                   
                 CGACGGCGUCAACAUCACCGUCAACCUCAAGCCUACAGGC 
                   
               
               
                   
                 GGCCUCGCCAACGGCGUCCGCCGCUACGCCUCCCAGACCG 
                   
               
               
                   
                 AGCUCUACGACGCCCCUGGCUGGCUCAUCUGGACCUACCG 
                   
               
               
                   
                 CACCAGGACCACUGUCAAUUGCCUCAUCACCGACAUGAU 
                   
               
               
                   
                 GGCCAAGUCCAACUCCCCUUUCGACUUCUUCGUGACCACC 
                   
               
               
                   
                 ACAGGCCAGACCGUCGAGAUGUCGCCAUUCUAUGACGGC 
                   
               
               
                   
                 AAGAACAAGGAGACUUUCCACGAGAGAGCGGACUCUUUC 
                   
               
               
                   
                 CAUGUCCGCACCAACUACAAGAUUGUCGACUACGACAAC 
                   
               
               
                   
                 CGCGGCACCAAUCCACAGGGCGAGCGCCGCGCCUUCCUCG 
                   
               
               
                   
                 ACAAGGGCACCUACACCCUCUCCUGGAAGCUCGAGAACCG 
                   
               
               
                   
                 CACCGCCUACUGUCCUCUCCAGCACUGGCAGACCUUCGAU 
                   
               
               
                   
                 UCCACCAUCGCCACCGAGACUGGCAAGUCCAUCCAUUUCG 
                   
               
               
                   
                 UCACCGACGAAGGAACAUCUUCAUUCGUCACAAAUACAA 
                   
               
               
                   
                 CCGUGGGCAUCGAGCUCCCCGACGCCUUCAAGUGCAUCGA 
                   
               
               
                   
                 GGAGCAGGUCAACAAGACCAUGCACGAGAAGUACGAGGC 
                   
               
               
                   
                 CGUCCAGGACCGGUAUACAAAGGGCCAGGAGGCCAUCAC 
                   
               
               
                   
                 CUACUUCAUCACCAGCGGAGGUCUGCUUCUCGCCUGGCU 
                   
               
               
                   
                 UCCACUCACACCUCGCUCCCUGGCCACAGUGAAGAACCUC 
                   
               
               
                   
                 ACUGAGUUGACCACCCCAACAAGUUCCCCACCUAGUAGUC 
                   
               
               
                   
                 CUAGCCCUCCUGCUCCUUCCGCCGCCCGCGGCUCCACGCC 
                   
               
               
                   
                 UGCCGCCGUCCUGCGGAGACGGAGAAGGGACGCCGGCAA 
                   
               
               
                   
                 CGCUACCACUCCUGUGCCGCCGACAGCCCCUGGAAAGUCC 
                   
               
               
                   
                 CUAGGUACCCUCAACAACCCAGCGACAGUUCAGAUCCAG 
                   
               
               
                   
                 UUCGCCUACGACUCUCUCAGGCGCCAGAUCAACCGCAUGC 
                   
               
               
                   
                 UUGGUGACCUUGCCCGCGCCUGGUGCCUCGAGCAGAAGC 
                   
               
               
                   
                 GCCAGAACAUGGUGCUUCGUGAACUAACAAAGAUUAAUC 
                   
               
               
                   
                 CUACUACAGUGAUGUCCUCCAUCUACGGCAAGGCCGUCG 
                   
               
               
                   
                 CCGCCAAGCGCCUCGGCGACGUCAUCUCCGUCUCCCAGUG 
                   
               
               
                   
                 CGUCCCAGUGAACCAGGCUACCGUGACCCUCAGGAAGUCC 
                   
               
               
                   
                 AUGAGAGUGCCAGGCUCCGAGACAAUGUGCUACUCCCGC 
                   
               
               
                   
                 CCACUCGUCUCCUUCUCCUUCAUCAACGACACAAAGACCU 
                   
               
               
                   
                 ACGAGGGCCAGUUAGGCACUGACAACGAGAUCUUCUUGA 
                   
               
               
                   
                 CUAAGAAGAUGACUGAGGUAUGCCAGGCAACUUCUCAAU 
                   
               
               
                   
                 ACUACUUCCAGUCUGGAAAUGAGAUCCACGUAUAUAACG 
                   
               
               
                   
                 ACUACCACCACUUCAAGACUAUUGAACUCGACGGAAUUG 
                   
               
               
                   
                 CCACCCUCCAAACAUUCAUCUCACUGAAUACCUCCCUCAU 
                   
               
               
                   
                 CGAGAACAUCGACUUCGCCUCCCUCGAGCUGUAUAGCAG 
                   
               
               
                   
                 AGACGAGCAGCGCGCCUCCAACGUCUUCGACCUCGAGGGC 
                   
               
               
                   
                 AUCUUCCGCGAGUACAACUUCCAGGCACAGAACAUAGCC 
                   
               
               
                   
                 GGCCUCCGUAAGGAUCUGGACAAUGCCGUGUCCAACGGC 
                   
               
               
                   
                 CGCAACCAGUUCGUCGACGGUUUGGGUGAACUCAUGGAC 
                   
               
               
                   
                 UCUCUGGGCUCCGUCGGCCAGUCCAUAACUAACUUAGUC 
                   
               
               
                   
                 UCUACGGUGGGAGGCCUAUUCAGCAGCCUGGUGAGCGGC 
                   
               
               
                   
                 UUCAUCUCUUUCUUCAAGAACCCCUUCGGCGGCAUGCUC 
                   
               
               
                   
                 AUCCUCGUCCUCGUCGCCGGCGUCGUCAUACUGGUGAUC 
                   
               
               
                   
                 UCACUCACAAGGAGGACGCGCCAAAUGUCCCAGCAGCCA 
                   
               
               
                   
                 GUGCAGAUGCUCUACCCAGGCAUAGACGAGCUCGCUCAG 
                   
               
               
                   
                 CAGCACGCGUCGGGUGAGGGACCAGGCAUCAAUCCUAUC 
                   
               
               
                   
                 UCCAAGACUGAGCUGCAAGCCAUUAUGCUCGCCCUCCACG 
                   
               
               
                   
                 AGCAGAACCAGGAACAGAAGCGGGCCGCCCAGCGAGCUG 
                   
               
               
                   
                 CCGGCCCCUCCGUCGCCAGUAGGGCACUACAAGCCGCCCG 
                   
               
               
                   
                 GGACCGCUUCCCAGGCCUGAGAAGGAGGAGAUACCACGA 
                   
               
               
                   
                 CCCAGAGACAGCCGCUGCCCUCCUUGGCGAAGCAGAAACC 
                   
               
               
                   
                 GAGUUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MTRRRVLSVVVLLAALACRLGAQTPEQPAPPATTVQPTATRQ 
                 129 
               
               
                 amino acid 
                 QTSFPFRVCELSSHGDLFRFSSDIQCPSFGTRENHTEGLLMVFK 
                   
               
               
                 sequence 
                 DNIIPYSFKVRSYTKIVTNILIYNGWYADSVTNRHEEKFSVDSY 
                   
               
               
                   
                 ETDQMDTIYQCYNAVKMTKDGLTRVYVDRDGVNITVNLKPT 
                   
               
               
                   
                 GGLANGVRRYASQTELYDAPGWLIWTYRTRTTVNCLITDMM 
                   
               
               
                   
                 AKSNSPFDFFVTTTGQTVEMSPFYDGKNKETFHERADSFHVR 
                   
               
               
                   
                 TNYKIVDYDNRGTNPQGERRAFLDKGTYTLSWKLENRTAYC 
                   
               
               
                   
                 PLQHWQTFDSTIATETGKSIHFVTDEGTSSFVTNTTVGIELPDA 
                   
               
               
                   
                 FKCIEEQVNKTMHEKYEAVQDRYTKGQEAITYFITSGGLLLA 
                   
               
               
                   
                 WLPLTPRSLATVKNLTELTTPTSSPPSSPSPPAPSAARGSTPAA 
                   
               
               
                   
                 VLRRRRRDAGNATTPVPPTAPGKSLGTLNNPATVQIQFAYDSL 
                   
               
               
                   
                 RRQINRMLGDLARAWCLEQKRQNMVLRELTKINPTTVMSSIY 
                   
               
               
                   
                 GKAVAAKRLGDVISVSQCVPVNQATVTLRKSMRVPGSETMC 
                   
               
               
                   
                 YSRPLVSFSFINDTKTYEGQLGTDNEIFLTKKMTEVCQATSQY 
                   
               
               
                   
                 YFQSGNEIHVYNDYHHFKTIELDGIATLQTFISLNTSLIENIDFA 
                   
               
               
                   
                 SLELYSRDEQRASNVFDLEGIFREYNFQAQNIAGLRKDLDNAV 
                   
               
               
                   
                 SNGRNQFVDGLGELMDSLGSVGQSITNLVSTVGGLFSSLVSGF 
                   
               
               
                   
                 ISFFKNPFGGMLILVLVAGVVILVISLTRRTRQMSQQPVQMLY 
                   
               
               
                   
                 PGIDELAQQHASGEGPGINPISKTELQAIMLALHEQNQEQKRA 
                   
               
               
                   
                 AQRAAGPSVASRALQAARDRFPGLRRRRYHDPETAAALLGE 
                   
               
               
                   
                 AETEF 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 V3_NGM 6mut CR2_EBV_gp350_001 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 210 consists of from 5′ end to 3′ end: 
                 210 
               
               
                 5′ UTR SEQ ID NO: 1, mRNA ORF SEQ ID NO: 211, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3. 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                   1 
               
               
                   
                 AGAGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGAGGCAGCACUGCUGGUCUGCCAGUAUACAAUCCAG 
                 211 
               
               
                 Construct 
                 UCGCUCAUUCAUCUGACAGGCGAGGACCCCGGCUUCUUU 
                   
               
               
                 (excluding 
                 AACGUGGAAAUCCCAGAGUUCCCUUUCUAUCCAACCUGU 
                   
               
               
                 the stop 
                 AACGUUUGCACUGCUGACGUGAACGUGACAAUCAAUUUC 
                   
               
               
                 codon) 
                 GACGUGGGAGGAAAGAAGCAUCAGCUUGAUCUGGAUUUC 
                   
               
               
                   
                 GGCCAACUGACGCCACACACCAAAGCGGUGUACCAACCAA 
                   
               
               
                   
                 GGGGUGCAUUUGGAGGGAGCGAGAAUGCUGCGAAUCUGU 
                   
               
               
                   
                 UCUUACUGGAACUGCUUGGUGCAGGAGAGCUGGCACUAA 
                   
               
               
                   
                 CCAUGCGAAGCAAGAAGCUCCCCAUCAACGUUACCACCGG 
                   
               
               
                   
                 GGAAGAACAGCAAGUCAGCCUUGAAUCCGUGGAUGUGUA 
                   
               
               
                   
                 CUUUCAGGAUGUUUUCGGCACCAUGUGGUGUCACCACGC 
                   
               
               
                   
                 UGAGAUGCAGAAUCCCGUGUAUCUCAUACCCGAGACUGU 
                   
               
               
                   
                 ACCCUACAUCAAGUGGGAUAAUUGCAAUUCAGCGAAUAU 
                   
               
               
                   
                 UACGGCUGUGGUGAGGGCCCAGGGCUUGGACGUGACUCU 
                   
               
               
                   
                 GCCCUUAUCUCUACCUACUUCUGCCCAAGACUCCAAUUUC 
                   
               
               
                   
                 GCGGUCAAGACCGAGAUGCUCGGGAAUGAGAUCGAUAUC 
                   
               
               
                   
                 GAGUGCAUCAUGGAGGACGGUGAGAUAAGCCAGGUUCUG 
                   
               
               
                   
                 CCCGGCGACAACAAGUUCAAUAUCGCGUGUUCUGGCUAC 
                   
               
               
                   
                 GAGUCCCAUGUGCCUAGUGGUGGCAUACUCACAAGUACU 
                   
               
               
                   
                 UCUCCCGUAGCCACGCCCAUUCCCGGAACCGGAUACGCCU 
                   
               
               
                   
                 ACAGUCUGCGUCUGACCCCACGGCCUGUGUCCAGAUUCCU 
                   
               
               
                   
                 GGGUAACAAUAGUAUCUUAUACGUGUUUUAUAGCGGAAA 
                   
               
               
                   
                 CGGCCCUAAAGCGUCCGGAGGGGACUAUUGUAUUCAGAG 
                   
               
               
                   
                 UAAUAUCGUUUUCUCUGAUGAGAUUCCUGCCAGUCAGGA 
                   
               
               
                   
                 CAUGCCGACAAACACAACUGAUAUUACCUACGUGGGCGA 
                   
               
               
                   
                 CAAUGCCACGUAUUCAGUGCCCAUGGUCACGAGCGAGGA 
                   
               
               
                   
                 CGCCAAUUCACCAAAUGUUGCGGUAACAGCUUUCUGGGC 
                   
               
               
                   
                 CUGGCCAAAUAACACUGAGACUGACUUCAAAUGUAAGUG 
                   
               
               
                   
                 GACUUUGACCUCUGGAACUCCGUCGGGUUGCGAGAAUAU 
                   
               
               
                   
                 CAGCGGGGCCUUUGCUUCCAACAGGACUUUCGACAUCAC 
                   
               
               
                   
                 UGUCUCAGGGCUGGGGACAGCACCGAAGACAUUAAUCAU 
                   
               
               
                   
                 AACACGGACCGCCACCAACGCCGCGACUACAACCCAUAAG 
                   
               
               
                   
                 GUGAUCUUUUCCAAGGCACCUGAGUCCACCACUACCUCCC 
                   
               
               
                   
                 CGACUCUUAACACUACGGGCUUCGCUGAUCCCAACACCAC 
                   
               
               
                   
                 UACGGGGUUGCCUAGCUCGACACAUGUGCCGACGAACCU 
                   
               
               
                   
                 GACUGCCCCUGCAUCGACCGGGCCCACAGUUUCGACCGCC 
                   
               
               
                   
                 GAUGUGACAUCACCAACGCCCGCAGGUACAACCUCAGGC 
                   
               
               
                   
                 GCCAGCCCAGUGACCCCUUCCCCAAGCCCCUGGGAUAAUG 
                   
               
               
                   
                 GAACGGAGUCCAAGGCUCCUGAUAUGACUUCCUCUACCA 
                   
               
               
                   
                 GCCCCGUGACUACACCCACUCCCAACGCAACUAGCCCAAC 
                   
               
               
                   
                 CCCAGCUGUGACGACGCCCACCCCGAACGCGACAUCUCCC 
                   
               
               
                   
                 ACACCUGCUGUGACAACCCCAACCCCUAACGCCACUAGCC 
                   
               
               
                   
                 CUACCCUAGGUAAGACCAGUCCGACUAGCGCCGUUACAA 
                   
               
               
                   
                 CCCCUACCCCUAACGCAACCGGCCCGACCGUGGGCGAGAC 
                   
               
               
                   
                 UUCCCCGCAAGCCAAUGCGACAAAUCACACAUUGGGCGG 
                   
               
               
                   
                 GACCUCUCCUACACCAGUCGUUACAUCUCAGCCUAAGAAC 
                   
               
               
                   
                 GCUACCUCCGCUGUCACUACCGGACAGCACAACAUCACCA 
                   
               
               
                   
                 GCUCAAGCACGAGUUCCAUGAGCUUGCGGCCGAGCUCAA 
                   
               
               
                   
                 AUCCCGAGACCCUAUCACCAUCCACAUCAGACAACAGUAC 
                   
               
               
                   
                 UUCACACAUGCCACUCUUGACGAGCGCUCACCCCACCGGC 
                   
               
               
                   
                 GGCGAGAACAUCACCCAGGUGACUCCGGCGUCUAUUUCC 
                   
               
               
                   
                 ACCCACCACGUCAGCACGUCGUCUCCCGCACCGAGACCAG 
                   
               
               
                   
                 GGACGACUUCUCAGGCCAGUGGGCCUGGCAACUCCUCUA 
                   
               
               
                   
                 CAAGCACAAAGCCAGGCGAAGUUAACGUGACAAAGGGAA 
                   
               
               
                   
                 CGCCUCCCCAGAACGCAACCAGUCCUCAGGCGCCCAGCGG 
                   
               
               
                   
                 GCAGAAGACUGCGGUGCCAACUGUGACCAGCACCGGUGG 
                   
               
               
                   
                 CAAGGCCAACUCAACAACUGGAGGCAAGCAUACGACGGG 
                   
               
               
                   
                 GCACGGCGCCCGGACCUCCACUGAACCCACGACCGAUUAC 
                   
               
               
                   
                 GGAGGUGACAGCACAACACCGCGGCCACGAUAUAAUGCC 
                   
               
               
                   
                 ACCACUUAUCUGCCACCAUCCACAAGCUCCAAGCUGCGGC 
                   
               
               
                   
                 CACGGUGGACCUUCACAAGCCCACCCGUGACGACUGCCCA 
                   
               
               
                   
                 AGCGACGGUGCCAGUGCCACCUACAAGCCAGCCACGCUUC 
                   
               
               
                   
                 UCCAACCUUAGUAUGCUCGUUCUCCAGUGGGCCAGCCUU 
                   
               
               
                   
                 GCUGUUCUGACCCUCCUCCUGCUGCUCGUGAUGGCCGAU 
                   
               
               
                   
                 UGCGCCUUUAGGAGAAACCUCAGCACUAGCCACACGUAC 
                   
               
               
                   
                 ACCACGCCGCCCUACGAUGACGCCGAGACUUACGUC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MEAALLVCQYTIQSLIHLTGEDPGFFNVEIPEFPFYPTCNVCTA 
                  70 
               
               
                 amino acid 
                 DVNVTINFDVGGKKHQLDLDFGQLTPHTKAVYQPRGAFGGS 
                   
               
               
                 sequence 
                 ENAANLFLLELLGAGELALTMRSKKLPINVTTGEEQQVSLESV 
                   
               
               
                   
                 DVYFQDVFGTMWCHHAEMQNPVYLIPETVPYIKWDNCNSAN 
                   
               
               
                   
                 ITAVVRAQGLDVTLPLSLPTSAQDSNFAVKTEMLGNEIDIECI 
                   
               
               
                   
                 MEDGEISQVLPGDNKFNIACSGYESHVPSGGILTSTSPVATPIP 
                   
               
               
                   
                 GTGYAYSLRLTPRPVSRFLGNNSILYVFYSGNGPKASGGDYCI 
                   
               
               
                   
                 QSNIVFSDEIPASQDMPTNTTDITYVGDNATYSVPMVTSEDAN 
                   
               
               
                   
                 SPNVAVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFA 
                   
               
               
                   
                 SNRTFDITVSGLGTAPKTLIITRTATNAATTTHKVIFSKAPESTT 
                   
               
               
                   
                 TSPTLNTTGFADPNTTTGLPSSTHVPTNLTAPASTGPTVSTADV 
                   
               
               
                   
                 TSPTPAGTTSGASPVTPSPSPWDNGTESKAPDMTSSTSPVTTPT 
                   
               
               
                   
                 PNATSPTPAVTTPTPNATSPTPAVTTPTPNATSPTLGKTSPTSA 
                   
               
               
                   
                 VTTPTPNATGPTVGETSPQANATNHTLGGTSPTPVVTSQPKNA 
                   
               
               
                   
                 TSAVTTGQHNITSSSTSSMSLRPSSNPETLSPSTSDNSTSHMPLL 
                   
               
               
                   
                 TSAHPTGGENITQVTPASISTHHVSTSSPAPRPGTTSQASGPGN 
                   
               
               
                   
                 SSTSTKPGEVNVTKGTPPQNATSPQAPSGQKTAVPTVTSTGGK 
                   
               
               
                   
                 ANSTTGGKHTTGHGARTSTEPTTDYGGDSTTPRPRYNATTYL 
                   
               
               
                   
                 PPSTSSKLRPRWTFTSPPVTTAQATVPVPPTSQPRFSNLSMLVL 
                   
               
               
                   
                 QWASLAVLTLLLLLVMADCAFRRNLSTSHTYTTPPYDDAETY 
                   
               
               
                   
                 V 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV Linked Construct 1 (1723942) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 212 consists of from 5′ end to 3′ end: 
                 212 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 213, and 
                   
               
               
                 3′ UTR SEQ ID NO: 106. 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGUGAGCUUCAAGCAGGUGAGAGUGCCCCUGUUCACC 
                 213 
               
               
                 Construct 
                 GCCAUCGCCCUGGUGAUCGUGCUGCUGCUGGCCUACUUCC 
                   
               
               
                 (excluding 
                 UGCCACCCAGAGUGAGAGGCGGCGGCAGAGUGGCCGCCG 
                   
               
               
                 the stop 
                 CCGCCAUCACCUGGGUGCCCAAGCCCAACGUGGAGGUGU 
                   
               
               
                 codon) 
                 GGCCCGUGGACCCGCCACCUCCCGUGAACUUCAACAAGAC 
                   
               
               
                   
                 CGCCGAGCAGGAGUACGGCGACAAGGAGGUGAAGCUGCC 
                   
               
               
                   
                 CCACUGGACGCCGACCCUGCACACCUUCCAGGUGCCCCAG 
                   
               
               
                   
                 AACUACACCAAGGCCAACUGCACCUACUGCAACACCAGAG 
                   
               
               
                   
                 AGUACACCUUCAGCUACAAGGGCUGCUGCUUCUACUUCA 
                   
               
               
                   
                 CCAAGAAGAAGCACACCUGGAACGGCUGCUUCCAGGCCU 
                   
               
               
                   
                 GCGCCGAGCUGUACCCCUGCACCUACUUCUACGGCCCCAC 
                   
               
               
                   
                 CCCAGACAUCCUGCCCGUGGUGACCAGAAACCUGAACGCC 
                   
               
               
                   
                 AUCGAGAGCCUGUGGGUGGGCGUGUACAGAGUGGGCGAG 
                   
               
               
                   
                 GGCAACUGGACCAGCCUGGACGGCGGCACCUUCAAGGUG 
                   
               
               
                   
                 UACCAGAUCUUCGGCAGCCACUGCACCUACGUGAGCAAG 
                   
               
               
                   
                 UUCAGCACCGUGCCCGUGAGCCACCACGAGUGCAGCUUCC 
                   
               
               
                   
                 UGAAGCCCUGCCUGUGCGUGAGCCAGAGAAGCAACAGCG 
                   
               
               
                   
                 GCACUAGCGGCUCUAGCGGAUCAGGCAGCGGAGGGAGUG 
                   
               
               
                   
                 GAGGCUCAGGAGGGACCAGCGGCAGCAGUGGCUCCGGAA 
                   
               
               
                   
                 GCGGAGGCAGCCCCUGCUGCCACGUGACCCAGCUGAGAGC 
                   
               
               
                   
                 CCAGCACCUGCUGGCCCUGGAGAACAUCAGCGACAUCUAC 
                   
               
               
                   
                 CUGGUGAGCAACCAGACCUGCGACGGCUUCAGCCUGGCC 
                   
               
               
                   
                 AGCCUGAACAGCCCCAAGAACGGCAGCAACCAGCUGGUG 
                   
               
               
                   
                 AUCAGCAGAUGCGCCAACGGCCUGAACGUGGUGAGCUUC 
                   
               
               
                   
                 UUCAUCAGCAUCCUGAAGAGAAGCAGCAGCGCCCUGACC 
                   
               
               
                   
                 GGCCACCUGAGAGAGCUGCUGACCACCCUGGAGACCCUG 
                   
               
               
                   
                 UACGGCAGCUUCAGCGUGGAGGACCUGUUCGGCGCCAAC 
                   
               
               
                   
                 CUGAACAGAUACGCCUGGCACAGAGGCGGCGGAGGAGGU 
                   
               
               
                   
                 GGCUCCGGUGGAGGUGGCAGCGGAGGAGGAGGCAGUGGU 
                   
               
               
                   
                 GGCGGAGGCAGCCUUAGCGAGGUGAAGCUCCACUUGGAC 
                   
               
               
                   
                 AUCGAGGGCCACGCCAGCCACUACACCAUCCCCUGGACCG 
                   
               
               
                   
                 AGCUCAUGGCCAAGGUGCCCGGCCUUAGCCCCGAGGCCCU 
                   
               
               
                   
                 GUGGCGGGAGGCCAACGUGACCGAGGACCUGGCCAGCAU 
                   
               
               
                   
                 GCUGAACCGGUACAAGCUGAUCUACAAGACCAGCGGCAC 
                   
               
               
                   
                 CCUGGGCAUCGCCCUGGCCGAGCCCGUGGACAUCCCCGCC 
                   
               
               
                   
                 GUUAGCGAAGGCAGCAUGCAGGUGGACGCCAGCAAGGUG 
                   
               
               
                   
                 CACCCCGGCGUGAUCAGCGGCCUGAACAGCCCCGCCUGUA 
                   
               
               
                   
                 UGUUGAGCGCCCCACUGGAGAAGCAGCUGUUCUACUACA 
                   
               
               
                   
                 UCGGCACCAUGCUGCCCAACACCCGGCCCCACAGCUACGU 
                   
               
               
                   
                 GUUCUACCAGCUGCGGUGCCACCUGAGCUACGUUGCCCU 
                   
               
               
                   
                 GAGCAUCAACGGCGACAAGUUCCAGUACACCGGCGCCAU 
                   
               
               
                   
                 GACCAGCAAGUUCCUGAUGGGCACCUACAAGCGGGUCAC 
                   
               
               
                   
                 CGAGAAGGGCGACGAGCACGUGCUGUCACUGGUGUUCGG 
                   
               
               
                   
                 CAAGACCAAGGACCUGCCCGACCUGCGGGGCCCCUUCAGC 
                   
               
               
                   
                 UACCCUAGUUUGACCAGCGCCCAGAGCGGCGACUACAGC 
                   
               
               
                   
                 UUGGUGAUCGUGACCACCUUCGUGCACUACGCCAACUUC 
                   
               
               
                   
                 CACAACUACUUCGUGCCCAACCUGAAGGACAUGUUCAGC 
                   
               
               
                   
                 CGGGCCGUGACCAUGACUGCCGCUUCUUACGCCCGGUACG 
                   
               
               
                   
                 UGCUGCAGAAGCUGGUCCUGCUGGAGAUGAAGGGCGGCU 
                   
               
               
                   
                 GCCGGGAGCCCGAGCUGGACACCGAAACACUGACCACCAU 
                   
               
               
                   
                 GUUCGAGGUGAGCGUGGCCUUCUUCAAGGUGGGUCACGC 
                   
               
               
                   
                 GGUGGGCGAAACCGGCAACGGCUGCGUGGACUUACGCUG 
                   
               
               
                   
                 GCUGGCCAAGAGCUUCUUCGAGCUGACCGUGCUGAAGGA 
                   
               
               
                   
                 UAUCAUCGGCAUCUGCUACGGCGCCACCGUGAAGGGCAU 
                   
               
               
                   
                 GCAGAGCUACGGCCUGGAGCGGCUGGCCGCCAUGCUUAU 
                   
               
               
                   
                 GGCAACAGUGAAGAUGGAGGAGCUGGGACACCUGACAAC 
                   
               
               
                   
                 AGAGAAGCAGGAGUACGCCCUGAGACUGGCCACAGUGGG 
                   
               
               
                   
                 CUACCCAAAGGCCGGCGUGUACAGUGGACUGAUCGGCGG 
                   
               
               
                   
                 CGCAACCAGCGUGCUGCUAUCCGCUUACAACCGGCACCCG 
                   
               
               
                   
                 CUGUUCCAGCCCCUGCACACCGUGAUGCGGGAAACCCUGU 
                   
               
               
                   
                 UCAUCGGAAGCCACGUCGUGCUGCGGGAGCUGAGGCUGA 
                   
               
               
                   
                 ACGUAACCACCCAGGGCCCUAAUCUGGCCCUGUAUCAGCU 
                   
               
               
                   
                 CCUCAGUACCGCCCUGUGCAGCGCCCUUGAGAUCGGCGAG 
                   
               
               
                   
                 GUGCUCAGAGGCCUGGCCCUCGGUACCGAGAGCGGCCUC 
                   
               
               
                   
                 UUCAGCCCAUGCUACUUAAGCCUGCGGUUCGACCUGACCC 
                   
               
               
                   
                 GGGACAAGUUGCUGAGCAUGGCCCCGCAGGAGGCCACAC 
                   
               
               
                   
                 UGGACCAGGCAGCUGUAUCCAACGCCGUGGACGGCUUCC 
                   
               
               
                   
                 UGGGCAGACUGUCCCUGGAACGGGAGGACCGGGACGCCU 
                   
               
               
                   
                 GGCACCUGCCUGCCUACAAGUGUGUGGAUCGGCUGGACA 
                   
               
               
                   
                 AGGUGCUGAUGAUCAUCCCUCUGAUUAAUGUCACCUUCA 
                   
               
               
                   
                 UCAUCAGCAGCGACCGGGAGGUGCGGGGAUCCGCCCUCU 
                   
               
               
                   
                 ACGAGGCCAGCACCACCUAUCUGAGCAGCAGCCUGUUCCU 
                   
               
               
                   
                 GUCUCCUGUGAUCAUGAACAAGUGCAGCCAGGGCGCCGU 
                   
               
               
                   
                 GGCCGGCGAGCCCCGGCAGAUCCCCAAGAUCCAGAACUUC 
                   
               
               
                   
                 ACCCGGACCCAGAAGUCUUGCAUCUUCUGCGGCUUCGCCC 
                   
               
               
                   
                 UUUUGUCCUACGACGAGAAGGAGGGCUUGGAGACUACAA 
                   
               
               
                   
                 CCUACAUCACCAGCCAGGAGGUGCAGAACAGCAUCCUGU 
                   
               
               
                   
                 CAUCUAAUUACUUCGACUUCGACAACCUGCACGUUCAUU 
                   
               
               
                   
                 ACCUGCUCCUCACCACCAACGGUACCGUCAUGGAAAUCGC 
                   
               
               
                   
                 CGGACUGUACGAGGAGCGGGCCCAUGUUGUGCUGGCCAU 
                   
               
               
                   
                 CAUCCUGUACUUCAUCGCUUUCGCACUUGGCAUCUUCCU 
                   
               
               
                   
                 GGUGCACAAGAUCGUGAUGUUCUUCCUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCC 
                 106 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MVSFKQVRVPLFTAIALVIVLLLAYFLPPRVRGGGRVAAAAIT 
                 214 
               
               
                 amino acid 
                 WVPKPNVEVWPVDPPPPVNFNKTAEQEYGDKEVKLPHWTPT 
                   
               
               
                 sequence 
                 LHTFQVPQNYTKANCTYCNTREYTFSYKGCCFYFTKKKHTW 
                   
               
               
                   
                 NGCFQACAELYPCTYFYGPTPDILPVVTRNLNAIESLWVGVYR 
                   
               
               
                   
                 VGEGNWTSLDGGTFKVYQIFGSHCTYVSKFSTVPVSHHECSFL 
                   
               
               
                   
                 KPCLCVSQRSNSGTSGSSGSGSGGSGGSGGTSGSSGSGSGGSP 
                   
               
               
                   
                 CCHVTQLRAQHLLALENISDIYLVSNQTCDGFSLASLNSPKNG 
                   
               
               
                   
                 SNQLVISRCANGLNVVSFFISILKRSSSALTGHLRELLTTLETLY 
                   
               
               
                   
                 GSFSVEDLFGANLNRYAWHRGGGGGGSGGGGSGGGGSGGG 
                   
               
               
                   
                 GSLSEVKLHLDIEGHASHYTIPWTELMAKVPGLSPEALWREA 
                   
               
               
                   
                 NVTEDLASMLNRYKLIYKTSGTLGIALAEPVDIPAVSEGSMQV 
                   
               
               
                   
                 DASKVHPGVISGLNSPACMLSAPLEKQLFYYIGTMLPNTRPHS 
                   
               
               
                   
                 YVFYQLRCHLSYVALSINGDKFQYTGAMTSKFLMGTYKRVT 
                   
               
               
                   
                 EKGDEHVLSLVFGKTKDLPDLRGPFSYPSLTSAQSGDYSLVIV 
                   
               
               
                   
                 TTFVHYANFHNYFVPNLKDMFSRAVTMTAASYARYVLQKLV 
                   
               
               
                   
                 LLEMKGGCREPELDTETLTTMFEVSVAFFKVGHAVGETGNGC 
                   
               
               
                   
                 VDLRWLAKSFFELTVLKDIIGICYGATVKGMQSYGLERLAAM 
                   
               
               
                   
                 LMATVKMEELGHLTTEKQEYALRLATVGYPKAGVYSGLIGG 
                   
               
               
                   
                 ATSVLLSAYNRHPLFQPLHTVMRETLFIGSHVVLRELRLNVTT 
                   
               
               
                   
                 QGPNLALYQLLSTALCSALEIGEVLRGLALGTESGLFSPCYLSL 
                   
               
               
                   
                 RFDLTRDKLLSMAPQEATLDQAAVSNAVDGFLGRLSLEREDR 
                   
               
               
                   
                 DAWHLPAYKCVDRLDKVLMIIPLINVTFIISSDREVRGSALYE 
                   
               
               
                   
                 ASTTYLSSSLFLSPVIMNKCSQGAVAGEPRQIPKIQNFTRTQKS 
                   
               
               
                   
                 CIFCGFALLSYDEKEGLETTTYITSQEVQNSILSSNYFDFDNLH 
                   
               
               
                   
                 VHYLLLTTNGTVMEIAGLYEERAHVVLAIILYFIAFALGIFLVH 
                   
               
               
                   
                 KIVMFFL 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV Linked Construct 2 (1723941) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 215 consists of from 5′ end to 3′ end: 
                 215 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 216, and 
                   
               
               
                 3′ UTR SEQ ID NO: 106. 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGGUGAGCUUCAAGCAGGUGAGAGUGCCCCUGUUCACC 
                 216 
               
               
                 Construct 
                 GCCAUCGCCCUGGUGAUCGUGCUGCUGCUGGCCUACUUCC 
                   
               
               
                 (excluding 
                 UGCCACCCAGAGUGAGAGGCGGCGGCAGAGUGGCCGCCG 
                   
               
               
                 the stop 
                 CCGCCAUCACCUGGGUGCCCAAGCCCAACGUGGAGGUGU 
                   
               
               
                 codon) 
                 GGCCCGUGGACCCGCCACCUCCCGUGAACUUCAACAAGAC 
                   
               
               
                   
                 CGCCGAGCAGGAGUACGGCGACAAGGAGGUGAAGCUGCC 
                   
               
               
                   
                 CCACUGGACGCCGACCCUGCACACCUUCCAGGUGCCCCAG 
                   
               
               
                   
                 AACUACACCAAGGCCAACUGCACCUACUGCAACACCAGAG 
                   
               
               
                   
                 AGUACACCUUCAGCUACAAGGGCUGCUGCUUCUACUUCA 
                   
               
               
                   
                 CCAAGAAGAAGCACACCUGGAACGGCUGCUUCCAGGCCU 
                   
               
               
                   
                 GCGCCGAGCUGUACCCCUGCACCUACUUCUACGGCCCCAC 
                   
               
               
                   
                 CCCAGACAUCCUGCCCGUGGUGACCAGAAACCUGAACGCC 
                   
               
               
                   
                 AUCGAGAGCCUGUGGGUGGGCGUGUACAGAGUGGGCGAG 
                   
               
               
                   
                 GGCAACUGGACCAGCCUGGACGGCGGCACCUUCAAGGUG 
                   
               
               
                   
                 UACCAGAUCUUCGGCAGCCACUGCACCUACGUGAGCAAG 
                   
               
               
                   
                 UUCAGCACCGUGCCCGUGAGCCACCACGAGUGCAGCUUCC 
                   
               
               
                   
                 UGAAGCCCUGCCUGUGCGUGAGCCAGAGAAGCAACAGCG 
                   
               
               
                   
                 GAGGAGGAGGAUCUGGAGGCGGCGGAAGUGGUGGAGGAG 
                   
               
               
                   
                 GAAGCGGAGGAGGUGGCAGCGGAGGUGGUGGAAGUGGCG 
                   
               
               
                   
                 GCGGAGGAAGCCCCUGCUGCCACGUGACCCAGCUGAGAG 
                   
               
               
                   
                 CCCAGCACCUGCUGGCCCUGGAGAACAUCAGCGACAUCUA 
                   
               
               
                   
                 CCUGGUGAGCAACCAGACCUGCGACGGCUUCAGCCUGGCC 
                   
               
               
                   
                 AGCCUGAACAGCCCCAAGAACGGCAGCAACCAGCUGGUG 
                   
               
               
                   
                 AUCAGCAGAUGCGCCAACGGCCUGAACGUGGUGAGCUUC 
                   
               
               
                   
                 UUCAUCAGCAUCCUGAAGAGAAGCAGCAGCGCCCUGACC 
                   
               
               
                   
                 GGCCACCUGAGAGAGCUGCUGACCACCCUGGAGACCCUG 
                   
               
               
                   
                 UACGGCAGCUUCAGCGUGGAGGACCUGUUCGGCGCCAAC 
                   
               
               
                   
                 CUGAACAGAUACGCCUGGCACAGAGGCGGCGGAGGAGGU 
                   
               
               
                   
                 GGCUCCGGUGGAGGUGGCAGCGGAGGAGGAGGCAGUGGU 
                   
               
               
                   
                 GGCGGAGGCAGCCUUAGCGAGGUGAAGCUCCACUUGGAC 
                   
               
               
                   
                 AUCGAGGGCCACGCCAGCCACUACACCAUCCCCUGGACCG 
                   
               
               
                   
                 AGCUCAUGGCCAAGGUGCCCGGCCUUAGCCCCGAGGCCCU 
                   
               
               
                   
                 GUGGCGGGAGGCCAACGUGACCGAGGACCUGGCCAGCAU 
                   
               
               
                   
                 GCUGAACCGGUACAAGCUGAUCUACAAGACCAGCGGCAC 
                   
               
               
                   
                 CCUGGGCAUCGCCCUGGCCGAGCCCGUGGACAUCCCCGCC 
                   
               
               
                   
                 GUUAGCGAAGGCAGCAUGCAGGUGGACGCCAGCAAGGUG 
                   
               
               
                   
                 CACCCCGGCGUGAUCAGCGGCCUGAACAGCCCCGCCUGUA 
                   
               
               
                   
                 UGUUGAGCGCCCCACUGGAGAAGCAGCUGUUCUACUACA 
                   
               
               
                   
                 UCGGCACCAUGCUGCCCAACACCCGGCCCCACAGCUACGU 
                   
               
               
                   
                 GUUCUACCAGCUGCGGUGCCACCUGAGCUACGUUGCCCU 
                   
               
               
                   
                 GAGCAUCAACGGCGACAAGUUCCAGUACACCGGCGCCAU 
                   
               
               
                   
                 GACCAGCAAGUUCCUGAUGGGCACCUACAAGCGGGUCAC 
                   
               
               
                   
                 CGAGAAGGGCGACGAGCACGUGCUGUCACUGGUGUUCGG 
                   
               
               
                   
                 CAAGACCAAGGACCUGCCCGACCUGCGGGGCCCCUUCAGC 
                   
               
               
                   
                 UACCCUAGUUUGACCAGCGCCCAGAGCGGCGACUACAGC 
                   
               
               
                   
                 UUGGUGAUCGUGACCACCUUCGUGCACUACGCCAACUUC 
                   
               
               
                   
                 CACAACUACUUCGUGCCCAACCUGAAGGACAUGUUCAGC 
                   
               
               
                   
                 CGGGCCGUGACCAUGACUGCCGCUUCUUACGCCCGGUACG 
                   
               
               
                   
                 UGCUGCAGAAGCUGGUCCUGCUGGAGAUGAAGGGCGGCU 
                   
               
               
                   
                 GCCGGGAGCCCGAGCUGGACACCGAAACACUGACCACCAU 
                   
               
               
                   
                 GUUCGAGGUGAGCGUGGCCUUCUUCAAGGUGGGUCACGC 
                   
               
               
                   
                 GGUGGGCGAAACCGGCAACGGCUGCGUGGACUUACGCUG 
                   
               
               
                   
                 GCUGGCCAAGAGCUUCUUCGAGCUGACCGUGCUGAAGGA 
                   
               
               
                   
                 UAUCAUCGGCAUCUGCUACGGCGCCACCGUGAAGGGCAU 
                   
               
               
                   
                 GCAGAGCUACGGCCUGGAGCGGCUGGCCGCCAUGCUUAU 
                   
               
               
                   
                 GGCAACAGUGAAGAUGGAGGAGCUGGGACACCUGACAAC 
                   
               
               
                   
                 AGAGAAGCAGGAGUACGCCCUGAGACUGGCCACAGUGGG 
                   
               
               
                   
                 CUACCCAAAGGCCGGCGUGUACAGUGGACUGAUCGGCGG 
                   
               
               
                   
                 CGCAACCAGCGUGCUGCUAUCCGCUUACAACCGGCACCCG 
                   
               
               
                   
                 CUGUUCCAGCCCCUGCACACCGUGAUGCGGGAAACCCUGU 
                   
               
               
                   
                 UCAUCGGAAGCCACGUCGUGCUGCGGGAGCUGAGGCUGA 
                   
               
               
                   
                 ACGUAACCACCCAGGGCCCUAAUCUGGCCCUGUAUCAGCU 
                   
               
               
                   
                 CCUCAGUACCGCCCUGUGCAGCGCCCUUGAGAUCGGCGAG 
                   
               
               
                   
                 GUGCUCAGAGGCCUGGCCCUCGGUACCGAGAGCGGCCUC 
                   
               
               
                   
                 UUCAGCCCAUGCUACUUAAGCCUGCGGUUCGACCUGACCC 
                   
               
               
                   
                 GGGACAAGUUGCUGAGCAUGGCCCCGCAGGAGGCCACAC 
                   
               
               
                   
                 UGGACCAGGCAGCUGUAUCCAACGCCGUGGACGGCUUCC 
                   
               
               
                   
                 UGGGCAGACUGUCCCUGGAACGGGAGGACCGGGACGCCU 
                   
               
               
                   
                 GGCACCUGCCUGCCUACAAGUGUGUGGAUCGGCUGGACA 
                   
               
               
                   
                 AGGUGCUGAUGAUCAUCCCUCUGAUUAAUGUCACCUUCA 
                   
               
               
                   
                 UCAUCAGCAGCGACCGGGAGGUGCGGGGAUCCGCCCUCU 
                   
               
               
                   
                 ACGAGGCCAGCACCACCUAUCUGAGCAGCAGCCUGUUCCU 
                   
               
               
                   
                 GUCUCCUGUGAUCAUGAACAAGUGCAGCCAGGGCGCCGU 
                   
               
               
                   
                 GGCCGGCGAGCCCCGGCAGAUCCCCAAGAUCCAGAACUUC 
                   
               
               
                   
                 ACCCGGACCCAGAAGUCUUGCAUCUUCUGCGGCUUCGCCC 
                   
               
               
                   
                 UUUUGUCCUACGACGAGAAGGAGGGCUUGGAGACUACAA 
                   
               
               
                   
                 CCUACAUCACCAGCCAGGAGGUGCAGAACAGCAUCCUGU 
                   
               
               
                   
                 CAUCUAAUUACUUCGACUUCGACAACCUGCACGUUCAUU 
                   
               
               
                   
                 ACCUGCUCCUCACCACCAACGGUACCGUCAUGGAAAUCGC 
                   
               
               
                   
                 CGGACUGUACGAGGAGCGGGCCCAUGUUGUGCUGGCCAU 
                   
               
               
                   
                 CAUCCUGUACUUCAUCGCUUUCGCACUUGGCAUCUUCCU 
                   
               
               
                   
                 GGUGCACAAGAUCGUGAUGUUCUUCCUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCC 
                 106 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MVSFKQVRVPLFTAIALVIVLLLAYFLPPRVRGGGRVAAAAIT 
                 217 
               
               
                 amino acid 
                 WVPKPNVEVWPVDPPPPVNFNKTAEQEYGDKEVKLPHWTPT 
                   
               
               
                 sequence 
                 LHTFQVPQNYTKANCTYCNTREYTFSYKGCCFYFTKKKHTW 
                   
               
               
                   
                 NGCFQACAELYPCTYFYGPTPDILPVVTRNLNAIESLWVGVYR 
                   
               
               
                   
                 VGEGNWTSLDGGTFKVYQIFGSHCTYVSKFSTVPVSHHECSFL 
                   
               
               
                   
                 KPCLCVSQRSNSGGGGSGGGGSGGGGSGGGGSGGGGSGGGG 
                   
               
               
                   
                 SPCCHVTQLRAQHLLALENISDIYLVSNQTCDGFSLASLNSPK 
                   
               
               
                   
                 NGSNQLVISRCANGLNVVSFFISILKRSSSALTGHLRELLTTLET 
                   
               
               
                   
                 LYGSFSVEDLFGANLNRYAWHRGGGGGGSGGGGSGGGGSG 
                   
               
               
                   
                 GGGSLSEVKLHLDIEGHASHYTIPWTELMAKVPGLSPEALWR 
                   
               
               
                   
                 EANVTEDLASMLNRYKLIYKTSGTLGIALAEPVDIPAVSEGSM 
                   
               
               
                   
                 QVDASKVHPGVISGLNSPACMLSAPLEKQLFYYIGTMLPNTRP 
                   
               
               
                   
                 HSYVFYQLRCHLSYVALSINGDKFQYTGAMTSKFLMGTYKR 
                   
               
               
                   
                 VTEKGDEHVLSLVFGKTKDLPDLRGPFSYPSLTSAQSGDYSLV 
                   
               
               
                   
                 IVTTFVHYANFHNYFVPNLKDMFSRAVTMTAASYARYVLQK 
                   
               
               
                   
                 LVLLEMKGGCREPELDTETLTTMFEVSVAFFKVGHAVGETGN 
                   
               
               
                   
                 GCVDLRWLAKSFFELTVLKDIIGICYGATVKGMQSYGLERLA 
                   
               
               
                   
                 AMLMATVKMEELGHLTTEKQEYALRLATVGYPKAGVYSGLI 
                   
               
               
                   
                 GGATSVLLSAYNRHPLFQPLHTVMRETLFIGSHVVLRELRLNV 
                   
               
               
                   
                 TTQGPNLALYQLLSTALCSALEIGEVLRGLALGTESGLFSPCYL 
                   
               
               
                   
                 SLRFDLTRDKLLSMAPQEATLDQAAVSNAVDGFLGRLSLERE 
                   
               
               
                   
                 DRDAWHLPAYKCVDRLDKVLMIIPLINVTFIISSDREVRGSAL 
                   
               
               
                   
                 YEASTTYLSSSLFLSPVIMNKCSQGAVAGEPRQIPKIQNFTRTQ 
                   
               
               
                   
                 KSCIFCGFALLSYDEKEGLETTTYITSQEVQNSILSSNYFDFDN 
                   
               
               
                   
                 LHVHYLLLTTNGTVMEIAGLYEERAHVVLAIILYFIAFALGIFL 
                   
               
               
                   
                 VHKIVMFFL 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV Linked Construct 3 (1723940) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 218 consists of from 5′ end to 3′ end: 
                 218 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 219, and 
                   
               
               
                 3′ UTR SEQ ID NO: 106. 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGAGCCGUGGGCGUGUUCCUGGCCAUCUGCCUGGUG 
                 219 
               
               
                 Construct 
                 ACCAUCUUCGUGCUGCCCACCUGGGGCAACUGGGCCUACC 
                   
               
               
                 (excluding 
                 CCUGCUGCCACGUGACCCAGCUGAGAGCCCAGCACCUGCU 
                   
               
               
                 the stop 
                 GGCCCUGGAGAACAUCAGCGACAUCUACCUGGUGAGCAA 
                   
               
               
                 codon) 
                 CCAGACCUGCGACGGCUUCAGCCUGGCCAGCCUGAACAGC 
                   
               
               
                   
                 CCCAAGAACGGCAGCAACCAGCUGGUGAUCAGCAGAUGC 
                   
               
               
                   
                 GCCAACGGCCUGAACGUGGUGAGCUUCUUCAUCAGCAUC 
                   
               
               
                   
                 CUGAAGAGAAGCAGCAGCGCCCUGACCGGCCACCUGAGA 
                   
               
               
                   
                 GAGCUGCUGACCACCCUGGAGACCCUGUACGGCAGCUUC 
                   
               
               
                   
                 AGCGUGGAGGACCUGUUCGGCGCCAACCUGAACAGAUAC 
                   
               
               
                   
                 GCCUGGCACAGAGGCGGCGGAGGAGGUGGCAGCGGAGGA 
                   
               
               
                   
                 GGAGGGUCUGGAGGUGGCGGCAGCCUUAGCGAGGUGAAG 
                   
               
               
                   
                 CUCCACUUGGACAUCGAGGGCCACGCCAGCCACUACACCA 
                   
               
               
                   
                 UCCCCUGGACCGAGCUCAUGGCCAAGGUGCCCGGCCUUAG 
                   
               
               
                   
                 CCCCGAGGCCCUGUGGCGGGAGGCCAACGUGACCGAGGA 
                   
               
               
                   
                 CCUGGCCAGCAUGCUGAACCGGUACAAGCUGAUCUACAA 
                   
               
               
                   
                 GACCAGCGGCACCCUGGGCAUCGCCCUGGCCGAGCCCGUG 
                   
               
               
                   
                 GACAUCCCCGCCGUUAGCGAAGGCAGCAUGCAGGUGGAC 
                   
               
               
                   
                 GCCAGCAAGGUGCACCCCGGCGUGAUCAGCGGCCUGAAC 
                   
               
               
                   
                 AGCCCCGCCUGUAUGUUGAGCGCCCCACUGGAGAAGCAG 
                   
               
               
                   
                 CUGUUCUACUACAUCGGCACCAUGCUGCCCAACACCCGGC 
                   
               
               
                   
                 CCCACAGCUACGUGUUCUACCAGCUGCGGUGCCACCUGAG 
                   
               
               
                   
                 CUACGUUGCCCUGAGCAUCAACGGCGACAAGUUCCAGUA 
                   
               
               
                   
                 CACCGGCGCCAUGACCAGCAAGUUCCUGAUGGGCACCUAC 
                   
               
               
                   
                 AAGCGGGUCACCGAGAAGGGCGACGAGCACGUGCUGUCA 
                   
               
               
                   
                 CUGGUGUUCGGCAAGACCAAGGACCUGCCCGACCUGCGG 
                   
               
               
                   
                 GGCCCCUUCAGCUACCCUAGUUUGACCAGCGCCCAGAGCG 
                   
               
               
                   
                 GCGACUACAGCUUGGUGAUCGUGACCACCUUCGUGCACU 
                   
               
               
                   
                 ACGCCAACUUCCACAACUACUUCGUGCCCAACCUGAAGGA 
                   
               
               
                   
                 CAUGUUCAGCCGGGCCGUGACCAUGACUGCCGCUUCUUA 
                   
               
               
                   
                 CGCCCGGUACGUGCUGCAGAAGCUGGUCCUGCUGGAGAU 
                   
               
               
                   
                 GAAGGGCGGCUGCCGGGAGCCCGAGCUGGACACCGAAAC 
                   
               
               
                   
                 ACUGACCACCAUGUUCGAGGUGAGCGUGGCCUUCUUCAA 
                   
               
               
                   
                 GGUGGGUCACGCGGUGGGCGAAACCGGCAACGGCUGCGU 
                   
               
               
                   
                 GGACUUACGCUGGCUGGCCAAGAGCUUCUUCGAGCUGAC 
                   
               
               
                   
                 CGUGCUGAAGGAUAUCAUCGGCAUCUGCUACGGCGCCAC 
                   
               
               
                   
                 CGUGAAGGGCAUGCAGAGCUACGGCCUGGAGCGGCUGGC 
                   
               
               
                   
                 CGCCAUGCUUAUGGCAACAGUGAAGAUGGAGGAGCUGGG 
                   
               
               
                   
                 ACACCUGACAACAGAGAAGCAGGAGUACGCCCUGAGACU 
                   
               
               
                   
                 GGCCACAGUGGGCUACCCAAAGGCCGGCGUGUACAGUGG 
                   
               
               
                   
                 ACUGAUCGGCGGCGCAACCAGCGUGCUGCUAUCCGCUUA 
                   
               
               
                   
                 CAACCGGCACCCGCUGUUCCAGCCCCUGCACACCGUGAUG 
                   
               
               
                   
                 CGGGAAACCCUGUUCAUCGGAAGCCACGUCGUGCUGCGG 
                   
               
               
                   
                 GAGCUGAGGCUGAACGUAACCACCCAGGGCCCUAAUCUG 
                   
               
               
                   
                 GCCCUGUAUCAGCUCCUCAGUACCGCCCUGUGCAGCGCCC 
                   
               
               
                   
                 UUGAGAUCGGCGAGGUGCUCAGAGGCCUGGCCCUCGGUA 
                   
               
               
                   
                 CCGAGAGCGGCCUCUUCAGCCCAUGCUACUUAAGCCUGCG 
                   
               
               
                   
                 GUUCGACCUGACCCGGGACAAGUUGCUGAGCAUGGCCCC 
                   
               
               
                   
                 GCAGGAGGCCACACUGGACCAGGCAGCUGUAUCCAACGC 
                   
               
               
                   
                 CGUGGACGGCUUCCUGGGCAGACUGUCCCUGGAACGGGA 
                   
               
               
                   
                 GGACCGGGACGCCUGGCACCUGCCUGCCUACAAGUGUGU 
                   
               
               
                   
                 GGAUCGGCUGGACAAGGUGCUGAUGAUCAUCCCUCUGAU 
                   
               
               
                   
                 UAAUGUCACCUUCAUCAUCAGCAGCGACCGGGAGGUGCG 
                   
               
               
                   
                 GGGAUCCGCCCUCUACGAGGCCAGCACCACCUAUCUGAGC 
                   
               
               
                   
                 AGCAGCCUGUUCCUGUCUCCUGUGAUCAUGAACAAGUGC 
                   
               
               
                   
                 AGCCAGGGCGCCGUGGCCGGCGAGCCCCGGCAGAUCCCCA 
                   
               
               
                   
                 AGAUCCAGAACUUCACCCGGACCCAGAAGUCUUGCAUCU 
                   
               
               
                   
                 UCUGCGGCUUCGCCCUUUUGUCCUACGACGAGAAGGAGG 
                   
               
               
                   
                 GCUUGGAGACUACAACCUACAUCACCAGCCAGGAGGUGC 
                   
               
               
                   
                 AGAACAGCAUCCUGUCAUCUAAUUACUUCGACUUCGACA 
                   
               
               
                   
                 ACCUGCACGUUCAUUACCUGCUCCUCACCACCAACGGUAC 
                   
               
               
                   
                 CGUCAUGGAAAUCGCCGGACUGUACGAGGAGCGGGCCCA 
                   
               
               
                   
                 UGUUGUGCUGGCCAUCAUCCUGUACUUCAUCGCUUUCGC 
                   
               
               
                   
                 ACUUGGCAUCUUCCUGGUGCACAAGAUCGUGAUGUUCUU 
                   
               
               
                   
                 CCUG 
                   
               
               
                   
               
               
                 3′UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCC 
                 106 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MRAVGVFLAICLVTIFVLPTWGNWAYPCCHVTQLRAQHLLA 
                 220 
               
               
                 amino acid 
                 LENISDIYLVSNQTCDGFSLASLNSPKNGSNQLVISRCANGLNV 
                   
               
               
                 sequence 
                 VSFFISILKRSSSALTGHLRELLTTLETLYGSFSVEDLFGANLNR 
                   
               
               
                   
                 YAWHRGGGGGGSGGGGSGGGGSLSEVKLHLDIEGHASHYTIP 
                   
               
               
                   
                 WTELMAKVPGLSPEALWREANVTEDLASMLNRYKLIYKTSG 
                   
               
               
                   
                 TLGIALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSPACMLS 
                   
               
               
                   
                 APLEKQLFYYIGTMLPNTRPHSYVFYQLRCHLSYVALSINGDK 
                   
               
               
                   
                 FQYTGAMTSKFLMGTYKRVTEKGDEHVLSLVFGKTKDLPDL 
                   
               
               
                   
                 RGPFSYPSLTSAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMF 
                   
               
               
                   
                 SRAVTMTAASYARYVLQKLVLLEMKGGCREPELDTETLTTM 
                   
               
               
                   
                 FEVSVAFFKVGHAVGETGNGCVDLRWLAKSFFELTVLKDIIGI 
                   
               
               
                   
                 CYGATVKGMQSYGLERLAAMLMATVKMEELGHLTTEKQEY 
                   
               
               
                   
                 ALRLATVGYPKAGVYSGLIGGATSVLLSAYNRHPLFQPLHTV 
                   
               
               
                   
                 MRETLFIGSHVVLRELRLNVTTQGPNLALYQLLSTALCSALEI 
                   
               
               
                   
                 GEVLRGLALGTESGLFSPCYLSLRFDLTRDKLLSMAPQEATLD 
                   
               
               
                   
                 QAAVSNAVDGFLGRLSLEREDRDAWHLPAYKCVDRLDKVL 
                   
               
               
                   
                 MIIPLINVTFIISSDREVRGSALYEASTTYLSSSLFLSPVIMNKCS 
                   
               
               
                   
                 QGAVAGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKEGLETTT 
                   
               
               
                   
                 YITSQEVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEIAGLYE 
                   
               
               
                   
                 ERAHVVLAIILYFIAFALGIFLVHKIVMFFL 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV Linked Construct 4 (1723939) 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 221 consists of from 5′ end to 3′ end: 
                 221 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 222, and 
                   
               
               
                 3′ UTR SEQ ID NO: 106. 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGAGCCGUGGGCGUGUUCCUGGCCAUCUGCCUGGUG 
                 222 
               
               
                 Construct 
                 ACCAUCUUCGUGCUGCCCACCUGGGGCAACUGGGCCUACC 
                   
               
               
                 (excluding 
                 CCUGCUGCCACGUGACCCAGCUGAGAGCCCAGCACCUGCU 
                   
               
               
                 the stop 
                 GGCCCUGGAGAACAUCAGCGACAUCUACCUGGUGAGCAA 
                   
               
               
                 codon) 
                 CCAGACCUGCGACGGCUUCAGCCUGGCCAGCCUGAACAGC 
                   
               
               
                   
                 CCCAAGAACGGCAGCAACCAGCUGGUGAUCAGCAGAUGC 
                   
               
               
                   
                 GCCAACGGCCUGAACGUGGUGAGCUUCUUCAUCAGCAUC 
                   
               
               
                   
                 CUGAAGAGAAGCAGCAGCGCCCUGACCGGCCACCUGAGA 
                   
               
               
                   
                 GAGCUGCUGACCACCCUGGAGACCCUGUACGGCAGCUUC 
                   
               
               
                   
                 AGCGUGGAGGACCUGUUCGGCGCCAACCUGAACAGAUAC 
                   
               
               
                   
                 GCCUGGCACAGAGGCGGCGGAGGAGGUGGCUCCGGUGGA 
                   
               
               
                   
                 GGUGGCAGCGGAGGAGGAGGCAGUGGUGGCGGAGGCAGC 
                   
               
               
                   
                 CUUAGCGAGGUGAAGCUCCACUUGGACAUCGAGGGCCAC 
                   
               
               
                   
                 GCCAGCCACUACACCAUCCCCUGGACCGAGCUCAUGGCCA 
                   
               
               
                   
                 AGGUGCCCGGCCUUAGCCCCGAGGCCCUGUGGCGGGAGG 
                   
               
               
                   
                 CCAACGUGACCGAGGACCUGGCCAGCAUGCUGAACCGGU 
                   
               
               
                   
                 ACAAGCUGAUCUACAAGACCAGCGGCACCCUGGGCAUCG 
                   
               
               
                   
                 CCCUGGCCGAGCCCGUGGACAUCCCCGCCGUUAGCGAAGG 
                   
               
               
                   
                 CAGCAUGCAGGUGGACGCCAGCAAGGUGCACCCCGGCGU 
                   
               
               
                   
                 GAUCAGCGGCCUGAACAGCCCCGCCUGUAUGUUGAGCGC 
                   
               
               
                   
                 CCCACUGGAGAAGCAGCUGUUCUACUACAUCGGCACCAU 
                   
               
               
                   
                 GCUGCCCAACACCCGGCCCCACAGCUACGUGUUCUACCAG 
                   
               
               
                   
                 CUGCGGUGCCACCUGAGCUACGUUGCCCUGAGCAUCAAC 
                   
               
               
                   
                 GGCGACAAGUUCCAGUACACCGGCGCCAUGACCAGCAAG 
                   
               
               
                   
                 UUCCUGAUGGGCACCUACAAGCGGGUCACCGAGAAGGGC 
                   
               
               
                   
                 GACGAGCACGUGCUGUCACUGGUGUUCGGCAAGACCAAG 
                   
               
               
                   
                 GACCUGCCCGACCUGCGGGGCCCCUUCAGCUACCCUAGUU 
                   
               
               
                   
                 UGACCAGCGCCCAGAGCGGCGACUACAGCUUGGUGAUCG 
                   
               
               
                   
                 UGACCACCUUCGUGCACUACGCCAACUUCCACAACUACUU 
                   
               
               
                   
                 CGUGCCCAACCUGAAGGACAUGUUCAGCCGGGCCGUGAC 
                   
               
               
                   
                 CAUGACUGCCGCUUCUUACGCCCGGUACGUGCUGCAGAA 
                   
               
               
                   
                 GCUGGUCCUGCUGGAGAUGAAGGGCGGCUGCCGGGAGCC 
                   
               
               
                   
                 CGAGCUGGACACCGAAACACUGACCACCAUGUUCGAGGU 
                   
               
               
                   
                 GAGCGUGGCCUUCUUCAAGGUGGGUCACGCGGUGGGCGA 
                   
               
               
                   
                 AACCGGCAACGGCUGCGUGGACUUACGCUGGCUGGCCAA 
                   
               
               
                   
                 GAGCUUCUUCGAGCUGACCGUGCUGAAGGAUAUCAUCGG 
                   
               
               
                   
                 CAUCUGCUACGGCGCCACCGUGAAGGGCAUGCAGAGCUA 
                   
               
               
                   
                 CGGCCUGGAGCGGCUGGCCGCCAUGCUUAUGGCAACAGU 
                   
               
               
                   
                 GAAGAUGGAGGAGCUGGGACACCUGACAACAGAGAAGCA 
                   
               
               
                   
                 GGAGUACGCCCUGAGACUGGCCACAGUGGGCUACCCAAA 
                   
               
               
                   
                 GGCCGGCGUGUACAGUGGACUGAUCGGCGGCGCAACCAG 
                   
               
               
                   
                 CGUGCUGCUAUCCGCUUACAACCGGCACCCGCUGUUCCAG 
                   
               
               
                   
                 CCCCUGCACACCGUGAUGCGGGAAACCCUGUUCAUCGGA 
                   
               
               
                   
                 AGCCACGUCGUGCUGCGGGAGCUGAGGCUGAACGUAACC 
                   
               
               
                   
                 ACCCAGGGCCCUAAUCUGGCCCUGUAUCAGCUCCUCAGUA 
                   
               
               
                   
                 CCGCCCUGUGCAGCGCCCUUGAGAUCGGCGAGGUGCUCA 
                   
               
               
                   
                 GAGGCCUGGCCCUCGGUACCGAGAGCGGCCUCUUCAGCCC 
                   
               
               
                   
                 AUGCUACUUAAGCCUGCGGUUCGACCUGACCCGGGACAA 
                   
               
               
                   
                 GUUGCUGAGCAUGGCCCCGCAGGAGGCCACACUGGACCA 
                   
               
               
                   
                 GGCAGCUGUAUCCAACGCCGUGGACGGCUUCCUGGGCAG 
                   
               
               
                   
                 ACUGUCCCUGGAACGGGAGGACCGGGACGCCUGGCACCU 
                   
               
               
                   
                 GCCUGCCUACAAGUGUGUGGAUCGGCUGGACAAGGUGCU 
                   
               
               
                   
                 GAUGAUCAUCCCUCUGAUUAAUGUCACCUUCAUCAUCAG 
                   
               
               
                   
                 CAGCGACCGGGAGGUGCGGGGAUCCGCCCUCUACGAGGC 
                   
               
               
                   
                 CAGCACCACCUAUCUGAGCAGCAGCCUGUUCCUGUCUCCU 
                   
               
               
                   
                 GUGAUCAUGAACAAGUGCAGCCAGGGCGCCGUGGCCGGC 
                   
               
               
                   
                 GAGCCCCGGCAGAUCCCCAAGAUCCAGAACUUCACCCGGA 
                   
               
               
                   
                 CCCAGAAGUCUUGCAUCUUCUGCGGCUUCGCCCUUUUGU 
                   
               
               
                   
                 CCUACGACGAGAAGGAGGGCUUGGAGACUACAACCUACA 
                   
               
               
                   
                 UCACCAGCCAGGAGGUGCAGAACAGCAUCCUGUCAUCUA 
                   
               
               
                   
                 AUUACUUCGACUUCGACAACCUGCACGUUCAUUACCUGC 
                   
               
               
                   
                 UCCUCACCACCAACGGUACCGUCAUGGAAAUCGCCGGACU 
                   
               
               
                   
                 GUACGAGGAGCGGGCCCAUGUUGUGCUGGCCAUCAUCCU 
                   
               
               
                   
                 GUACUUCAUCGCUUUCGCACUUGGCAUCUUCCUGGUGCA 
                   
               
               
                   
                 CAAGAUCGUGAUGUUCUUCCUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCUAGCUUCUUGCC 
                 106 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MRAVGVFLAICLVTIFVLPTWGNWAYPCCHVTQLRAQHLLA 
                 223 
               
               
                 amino acid 
                 LENISDIYLVSNQTCDGFSLASLNSPKNGSNQLVISRCANGLNV 
                   
               
               
                 sequence 
                 VSFFISILKRSSSALTGHLRELLTTLETLYGSFSVEDLFGANLNR 
                   
               
               
                   
                 YAWHRGGGGGGSGGGGSGGGGSGGGGSLSEVKLHLDIEGHA 
                   
               
               
                   
                 SHYTIPWTELMAKVPGLSPEALWREANVTEDLASMLNRYKLI 
                   
               
               
                   
                 YKTSGTLGIALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSP 
                   
               
               
                   
                 ACMLSAPLEKQLFYYIGTMLPNTRPHSYVFYQLRCHLSYVAL 
                   
               
               
                   
                 SINGDKFQYTGAMTSKFLMGTYKRVTEKGDEHVLSLVFGKT 
                   
               
               
                   
                 KDLPDLRGPFSYPSLTSAQSGDYSLVIVTTFVHYANFHNYFVP 
                   
               
               
                   
                 NLKDMFSRAVTMTAASYARYVLQKLVLLEMKGGCREPELDT 
                   
               
               
                   
                 ETLTTMFEVSVAFFKVGHAVGETGNGCVDLRWLAKSFFELTV 
                   
               
               
                   
                 LKDIIGICYGATVKGMQSYGLERLAAMLMATVKMEELGHLT 
                   
               
               
                   
                 TEKQEYALRLATVGYPKAGVYSGLIGGATSVLLSAYNRHPLF 
                   
               
               
                   
                 QPLHTVMRETLFIGSHVVLRELRLNVTTQGPNLALYQLLSTAL 
                   
               
               
                   
                 CSALEIGEVLRGLALGTESGLFSPCYLSLRFDLTRDKLLSMAP 
                   
               
               
                   
                 QEATLDQAAVSNAVDGFLGRLSLEREDRDAWHLPAYKCVDR 
                   
               
               
                   
                 LDKVLMIIPLINVTFIISSDREVRGSALYEASTTYLSSSLFLSPVI 
                   
               
               
                   
                 MNKCSQGAVAGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKE 
                   
               
               
                   
                 GLETTTYITSQEVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEI 
                   
               
               
                   
                 AGLYEERAHVVLAIILYFIAFALGIFLVHKIVMFFL 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gH (BXLF2)_RX-UTRB 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 228 consists of from 5′ end to 3′ end: 
                 228 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 84, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGCAGUUGUUGUGCGUGUUCUGCCUCGUGUUACUCUGG 
                  84 
               
               
                 Construct 
                 GAGGUGGGCGCCGCCAGCCUUAGCGAGGUGAAGCUCCAC 
                   
               
               
                 (excluding 
                 UUGGACAUCGAGGGCCACGCCAGCCACUACACCAUCCCCU 
                   
               
               
                 the stop 
                 GGACCGAGCUCAUGGCCAAGGUGCCCGGCCUUAGCCCCGA 
                   
               
               
                 codon) 
                 GGCCCUGUGGCGGGAGGCCAACGUGACCGAGGACCUGGC 
                   
               
               
                   
                 CAGCAUGCUGAACCGGUACAAGCUGAUCUACAAGACCAG 
                   
               
               
                   
                 CGGCACCCUGGGCAUCGCCCUGGCCGAGCCCGUGGACAUC 
                   
               
               
                   
                 CCCGCCGUUAGCGAAGGCAGCAUGCAGGUGGACGCCAGC 
                   
               
               
                   
                 AAGGUGCACCCCGGCGUGAUCAGCGGCCUGAACAGCCCCG 
                   
               
               
                   
                 CCUGUAUGUUGAGCGCCCCACUGGAGAAGCAGCUGUUCU 
                   
               
               
                   
                 ACUACAUCGGCACCAUGCUGCCCAACACCCGGCCCCACAG 
                   
               
               
                   
                 CUACGUGUUCUACCAGCUGCGGUGCCACCUGAGCUACGU 
                   
               
               
                   
                 UGCCCUGAGCAUCAACGGCGACAAGUUCCAGUACACCGG 
                   
               
               
                   
                 CGCCAUGACCAGCAAGUUCCUGAUGGGCACCUACAAGCG 
                   
               
               
                   
                 GGUCACCGAGAAGGGCGACGAGCACGUGCUGUCACUGGU 
                   
               
               
                   
                 GUUCGGCAAGACCAAGGACCUGCCCGACCUGCGGGGCCCC 
                   
               
               
                   
                 UUCAGCUACCCUAGUUUGACCAGCGCCCAGAGCGGCGAC 
                   
               
               
                   
                 UACAGCUUGGUGAUCGUGACCACCUUCGUGCACUACGCC 
                   
               
               
                   
                 AACUUCCACAACUACUUCGUGCCCAACCUGAAGGACAUG 
                   
               
               
                   
                 UUCAGCCGGGCCGUGACCAUGACUGCCGCUUCUUACGCCC 
                   
               
               
                   
                 GGUACGUGCUGCAGAAGCUGGUCCUGCUGGAGAUGAAGG 
                   
               
               
                   
                 GCGGCUGCCGGGAGCCCGAGCUGGACACCGAAACACUGA 
                   
               
               
                   
                 CCACCAUGUUCGAGGUGAGCGUGGCCUUCUUCAAGGUGG 
                   
               
               
                   
                 GUCACGCGGUGGGCGAAACCGGCAACGGCUGCGUGGACU 
                   
               
               
                   
                 UACGCUGGCUGGCCAAGAGCUUCUUCGAGCUGACCGUGC 
                   
               
               
                   
                 UGAAGGAUAUCAUCGGCAUCUGCUACGGCGCCACCGUGA 
                   
               
               
                   
                 AGGGCAUGCAGAGCUACGGCCUGGAGCGGCUGGCCGCCA 
                   
               
               
                   
                 UGCUUAUGGCAACAGUGAAGAUGGAGGAGCUGGGACACC 
                   
               
               
                   
                 UGACAACAGAGAAGCAGGAGUACGCCCUGAGACUGGCCA 
                   
               
               
                   
                 CAGUGGGCUACCCAAAGGCCGGCGUGUACAGUGGACUGA 
                   
               
               
                   
                 UCGGCGGCGCAACCAGCGUGCUGCUAUCCGCUUACAACCG 
                   
               
               
                   
                 GCACCCGCUGUUCCAGCCCCUGCACACCGUGAUGCGGGAA 
                   
               
               
                   
                 ACCCUGUUCAUCGGAAGCCACGUCGUGCUGCGGGAGCUG 
                   
               
               
                   
                 AGGCUGAACGUAACCACCCAGGGCCCUAAUCUGGCCCUG 
                   
               
               
                   
                 UAUCAGCUCCUCAGUACCGCCCUGUGCAGCGCCCUUGAGA 
                   
               
               
                   
                 UCGGCGAGGUGCUCAGAGGCCUGGCCCUCGGUACCGAGA 
                   
               
               
                   
                 GCGGCCUCUUCAGCCCAUGCUACUUAAGCCUGCGGUUCG 
                   
               
               
                   
                 ACCUGACCCGGGACAAGUUGCUGAGCAUGGCCCCGCAGG 
                   
               
               
                   
                 AGGCCACACUGGACCAGGCAGCUGUAUCCAACGCCGUGG 
                   
               
               
                   
                 ACGGCUUCCUGGGCAGACUGUCCCUGGAACGGGAGGACC 
                   
               
               
                   
                 GGGACGCCUGGCACCUGCCUGCCUACAAGUGUGUGGAUC 
                   
               
               
                   
                 GGCUGGACAAGGUGCUGAUGAUCAUCCCUCUGAUUAAUG 
                   
               
               
                   
                 UCACCUUCAUCAUCAGCAGCGACCGGGAGGUGCGGGGAU 
                   
               
               
                   
                 CCGCCCUCUACGAGGCCAGCACCACCUAUCUGAGCAGCAG 
                   
               
               
                   
                 CCUGUUCCUGUCUCCUGUGAUCAUGAACAAGUGCAGCCA 
                   
               
               
                   
                 GGGCGCCGUGGCCGGCGAGCCCCGGCAGAUCCCCAAGAUC 
                   
               
               
                   
                 CAGAACUUCACCCGGACCCAGAAGUCUUGCAUCUUCUGC 
                   
               
               
                   
                 GGCUUCGCCCUUUUGUCCUACGACGAGAAGGAGGGCUUG 
                   
               
               
                   
                 GAGACUACAACCUACAUCACCAGCCAGGAGGUGCAGAAC 
                   
               
               
                   
                 AGCAUCCUGUCAUCUAAUUACUUCGACUUCGACAACCUG 
                   
               
               
                   
                 CACGUUCAUUACCUGCUCCUCACCACCAACGGUACCGUCA 
                   
               
               
                   
                 UGGAAAUCGCCGGACUGUACGAGGAGCGGGCCCAUGUUG 
                   
               
               
                   
                 UGCUGGCCAUCAUCCUGUACUUCAUCGCUUUCGCACUUG 
                   
               
               
                   
                 GCAUCUUCCUGGUGCACAAGAUCGUGAUGUUCUUCCUG 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MQLLCVFCLVLLWEVGAASLSEVKLHLDIEGHASHYTIPWTE 
                  38 
               
               
                 amino acid 
                 LMAKVPGLSPEALWREANVTEDLASMLNRYKLIYKTSGTLGI 
                   
               
               
                 sequence 
                 ALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSPACMLSAPLE 
                   
               
               
                   
                 KQLFYYIGTMLPNTRPHSYVFYQLRCHLSYVALSINGDKFQYT 
                   
               
               
                   
                 GAMTSKFLMGTYKRVTEKGDEHVLSLVFGKTKDLPDLRGPFS 
                   
               
               
                   
                 YPSLTSAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMFSRAV 
                   
               
               
                   
                 TMTAASYARYVLQKLVLLEMKGGCREPELDTETLTTMFEVS 
                   
               
               
                   
                 VAFFKVGHAVGETGNGCVDLRWLAKSFFELTVLKDIIGICYG 
                   
               
               
                   
                 ATVKGMQSYGLERLAAMLMATVKMEELGHLTTEKQEYALR 
                   
               
               
                   
                 LATVGYPKAGVYSGLIGGATSVLLSAYNRHPLFQPLHTVMRE 
                   
               
               
                   
                 TLFIGSHVVLRELRLNVTTQGPNLALYQLLSTALCSALEIGEVL 
                   
               
               
                   
                 RGLALGTESGLFSPCYLSLRFDLTRDKLLSMAPQEATLDQAA 
                   
               
               
                   
                 VSNAVDGFLGRLSLEREDRDAWHLPAYKCVDRLDKVLMIIPL 
                   
               
               
                   
                 INVTFIISSDREVRGSALYEASTTYLSSSLFLSPVIMNKCSQGAV 
                   
               
               
                   
                 AGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKEGLETTTYITSQ 
                   
               
               
                   
                 EVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEIAGLYEERAH 
                   
               
               
                   
                 VVLAIILYFIAFALGIFLVHKIVMFFL 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt 
                   
               
               
                   
               
            
           
           
               
            
               
                 EBV gL (B95-8)-UTRB 
               
            
           
           
               
               
            
               
                 SEQ ID NO: 229 consists of from 5′ end to 3′ end: 
                 229 
               
               
                 5′ UTR SEQ ID NO: 104, mRNA ORF SEQ ID NO: 85, and 
                   
               
               
                 3′ UTR SEQ ID NO: 3 
                   
               
               
                   
               
            
           
           
               
               
               
            
               
                 Chemistry 
                 1-methylpseudouridine 
                   
               
               
                   
               
               
                 Cap 
                 7mG(5′)ppp(5′)NlmpNp 
                   
               
               
                   
               
               
                 5′ UTR 
                 GGGAAAUAAGAGAGAAAAGAAGAGUAAGAAGAAAUAUA 
                 104 
               
               
                   
                 AGACCCCGGCGCCGCCACC 
                   
               
               
                   
               
               
                 ORF of mRNA 
                 AUGAGAGCCGUGGGCGUGUUCCUGGCCAUCUGCCUGGUG 
                  85 
               
               
                 Construct 
                 ACCAUCUUCGUGCUGCCCACCUGGGGCAACUGGGCCUACC 
                   
               
               
                 (excluding 
                 CCUGCUGCCACGUGACCCAGCUGAGAGCCCAGCACCUGCU 
                   
               
               
                 the stop 
                 GGCCCUGGAGAACAUCAGCGACAUCUACCUGGUGAGCAA 
                   
               
               
                 codon) 
                 CCAGACCUGCGACGGCUUCAGCCUGGCCAGCCUGAACAGC 
                   
               
               
                   
                 CCCAAGAACGGCAGCAACCAGCUGGUGAUCAGCAGAUGC 
                   
               
               
                   
                 GCCAACGGCCUGAACGUGGUGAGCUUCUUCAUCAGCAUC 
                   
               
               
                   
                 CUGAAGAGAAGCAGCAGCGCCCUGACCGGCCACCUGAGA 
                   
               
               
                   
                 GAGCUGCUGACCACCCUGGAGACCCUGUACGGCAGCUUC 
                   
               
               
                   
                 AGCGUGGAGGACCUGUUCGGCGCCAACCUGAACAGAUAC 
                   
               
               
                   
                 GCCUGGCACAGAGGCGGC 
                   
               
               
                   
               
               
                 3′ UTR 
                 UGAUAAUAGGCUGGAGCCUCGGUGGCCAUGCUUCUUGCC 
                   3 
               
               
                   
                 CCUUGGGCCUCCCCCCAGCCCCUCCUCCCCUUCCUGCACC 
                   
               
               
                   
                 CGUACCCCCGUGGUCUUUGAAUAAAGUCUGAGUGGGCGG 
                   
               
               
                   
                 C 
                   
               
               
                   
               
               
                 Corresponding 
                 MRAVGVFLAICLVTIFVLPTWGNWAYPCCHVTQLRAQHLLA 
                  36 
               
               
                 amino acid 
                 LENISDIYLVSNQTCDGFSLASLNSPKNGSNQLVISRCANGLNV 
                   
               
               
                 sequence 
                 VSFFISILKRSSSALTGHLRELLTTLETLYGSFSVEDLFGANLNR 
                   
               
               
                   
                 YAWHRGG 
                   
               
               
                   
               
               
                 PolyA tail 
                 100 nt