Patent Publication Number: US-7720613-B2

Title: Method for sequencing nucleic acids

Description:
FIELD OF THE INVENTION 
   This application claims the benefit of priority under 35 U.S.C. §119 of PCT/EP2002/010235 filed 12 Sep. 2002 and AT Application A 1444/2001 filed 14 Sep. 2001, the contents of which are hereby incorporated by reference. 
   The invention concerns a method for sequencing nucleic acids in which the values of the CID spectrum calculated for an assumed sequence are compared with the measured spectrum of the nucleic acid and the degree of correspondence is calculated. 
   In such methods nucleic acids are firstly ionized, selected in a first spectrometer and then fragmented by collision with gaseous atoms or molecules (referred to as collision induced dissociation (CID)). The fragmentation conforms to known rules which is why the mass spectrographic examination of the fragments allows an inference to be made about the initial ion. 
   DESCRIPTION OF RELATED ART 
   When nucleic acids are fragmented, ions (a-B ions) are formed at the 5′ end and at the 3′ end (w ions). A method for completely elucidating the entire sequence starting from the ends is described in Ni, J.; Pomerantz, S. C.; Rozenski, J.; Zhang, Y.; McCloskey J. A.  Anal. Chem.  1996, 68, 1989-99. Since an increasing number of insurmountable defects occur, this method is limited to nucleic acids of a small length (at most 15 mer). On the other hand a method of the type defined above is described in U.S. Pat. No. 6,017,693 (Yates et al) in which a library of peptides is examined with regard to whether they contain a compound corresponding to the measured spectrum. In this publication it is also suggested that the method be extended to oligonucleotides, but the method is typically restricted to 10 mers and below due to the enormous number of initial values that have to be examined. 
   SUMMARY OF INVENTION 
   The object of the invention is to make the known methods applicable to considerably longer sequences. This is essentially achieved in that the question that is asked is not primarily about the correct sequence but rather whether correspondence with the measured spectrum can be improved by systematically changing an initial sequence. 
   Hence the invention is characterized in that at least one position of the assumed sequence is modified and the degree of correspondence with the measured spectrum is recalculated. 
   Basically the invention allows an increasing approximation to the correct sequence starting with a random initial sequence that is assumed to be favourable by repeatedly applying the method. However, the actual strength of the method is in determining and locating point mutations and omissions or insertions of nucleic acid monomers in a known sequence. Particularly when the number of nucleotides is known, it is advantageous when each of the four possible nucleotides is used at a particular position and in each case the degree of correspondence with the spectrum is calculated. If this procedure is carried out for each position of the sequence and in each case the nucleotide having the best match is placed in the sequence, this finally results in an optimal match for the entire sequence. 
   Within the scope of the invention it is basically possible to use various methods to characterize the degree of correspondence between the calculated and measured spectrum by a numerical value. However, it is particularly simple when an algorithm is used to calculate the correspondence between the calculated and measured spectrum which is based on determining whether a measured value is present or not at the calculated position in the measured spectrum. 
   The m/z values for a certain fragment have to be calculated with high accuracy whereas the intensity of the measured values i.e. the frequency distribution of the various fragments is very difficult to predict. Hence it is appropriate to firstly refrain from predicting the intensities but rather to give the occurrence of a predicted measured value in the measured spectrum a higher rating when the measured intensity is higher. Since the m/z values have to be calculated with a higher degree of accuracy than their measurement, a difference value has to be defined up to which one regards a prediction as being fulfilled. This definition is based on the requirement that the number of false positives and false negatives has to be minimized. 
   Further details of the invention are discussed below on the basis of diagrams and embodiments. 

   
     BRIEF DESCRIPTION OF THE DRAWINGS 
       FIG. 1  elucidates the calculation of a fragmentation spectrum. 
       FIG. 2  concerns the procedure for comparing a calculated and a measured spectrum. 
       FIG. 3  relates to the optimization of the algorithm of  FIG. 2 . 
       FIG. 4  shows the relative importance of the factors of the algorithm of  FIG. 2 . 
       FIG. 5  is the measured spectrum of a 20 mer oligodeoxynucleotide. 
       FIGS. 6   a  and  6   b  show the deduction of a sequence having a maximum correspondence with  FIG. 5 . 
       FIG. 7  concerns the sequencing of a 51 mer oligodeoxynucleotide. 
       FIG. 8  concerns the examination of the sequence of an 80 mer oligodeoxynucleotide. 
   

   DETAILED DESCRIPTION OF THE INVENTION 
   Principle of Sequencing by Means of Tandem Mass Spectrometry 
   Nucleic acids are cleaved in a sequence-specific manner by collision-induced fragmentation whereby fragments of the precursor ion are formed which are designated according to the type of bond break in the phosphodiester group and the remaining charge. Whereas charge fragments having an intact 5′-terminal end are referred to as a, b, c, d fragments, charged fragments with an intact 3′-terminal end are referred to as w, x, y, z fragments ( FIG. 1   a ). The number of deoxyribose residues of a fragment is denoted with subscript numbers and the charge state is denoted with superscript numbers. Thus (a 5 ) 2−  represents a fragment of the a series which consists of five nucleotide units and is twice negatively charged. Fragments in which a base has been additionally cleaved off are designated by adding —B e.g. as (a n -B n ) m− . 
   Although the fragmentation can basically occur at any bond, it has turned out that there is a main fragmentation path for oligonucleotide anions. In this process there is firstly a cleavage of the 3′-C—O bond of the sugar which subsequently leads to a loss of the base and formation of w and complementary (a-B) ions ( FIG. 1   b ). Fragment ions of a particular type belong to a certain ion series where the mass differences within an ion series are determined by the masses of the bases in the sequence. Hence the sequence of a fragmented nucleic acid can be read off on the basis of the mass differences in an ion series ( FIG. 1   c ). 
   Comparative Sequencing Strategy 
   The starting point of the sequencing approach according to the invention is the fact that a complete new determination of the sequence is not necessary for many genome-specific applications. It is usually sufficient to verify a sequence or to find small deviations from a known sequence. In this context sequencing by means of tandem mass spectrometry (MS/MS) is an attractive alternative to the sequencing method of Sanger that is conventionally used. The reason for this is that sequence-specific data can be obtained within seconds by collision-induced fragmentation and subsequent analysis of the fragment ions. 
   The closeness of correspondence between a measured MS/MS spectrum and a set of fragment ions which can be calculated on the basis of an assumed reference, can be described by almost any mathematic algorithm. An algorithm will be elucidated as an example in which the correspondence between the measured spectrum and the calculated fragment ions is characterized by the fitness value (FS). The fitness incorporates the absolute difference A between the measured and calculated m/z values, the relative intensity I % of the fragment ions, the number of assigned fragments K and the number of nucleotide positions M that are not covered by a fragment ion. The value for fitness becomes smaller with an increasing degree of correspondence between the measured spectrum and the calculated m/z values. 
   In order to find the sequence which matches best with the experimental data of an MS/MS spectrum, the first reference sequence is sequentially varied by incorporating all four possible nucleotides A, T, G and C at each position of the sequence. The sequence having the lowest fitness value is then identified as the correct sequence. 
   Development of the Sequencing Algorithm 
     FIG. 2  shows the procedure for the comparative sequencing of nucleic acids. The input parameters are the reference sequence together with the charge state of the precursor ion on the one hand, and a list of experimentally determined m/z values and their relative intensities I % on the other hand. A list of monoisotopic m/z values for the a, a-B, w and w-B ion series taking into consideration all possible charge states of the fragment ions from 1-up to the charge state of the precursor ion, is calculated from the reference sequence (step  1 ,  FIG. 2 ). Subsequently the calculated m/z values and those found experimentally are compared (step  2   a,    FIG. 2 ). The result of this comparison is the number of fragment ions (K) that could be assigned a calculated m/z value and the sum of non-covered a and w positions (M). Non-covered a positions are those positions in the spectrum for which neither an a-B nor an a fragment of any possible charge state could be found. Similarly non-covered w positions are those positions in the spectrum for which neither a w-B nor a w fragment of any possible charge state could be assigned. In order to assign a fragment, the absolute value of the difference between the measured and the calculated m/z value must be smaller or equal to a tolerance value (Δ). Values for K and M are calculated in increasing order for all Δ values between 0.2 and 0.8 in 0.1 steps (step  2   b,    FIG. 2 ). Subsequently the optimal Δ value is selected on the basis that the number of false-positive (Δ too large) and false-negative (Δ too small) assignments is minimized (step  2   c,    FIG. 2 ). The optimum Δ value can be deduced from a plot of M versus Δ as the Δ value just before the curve reaches a constant value ( FIG. 3 ). 
   The next step of the algorithm is to calculate a match factor MF which reflects the quality of the assignment of the m/z values in terms of mass deviation and intensity (step  3 ,  FIG. 2 ). The match factor MF is defined as the sum of all quotients of the absolute mass deviation Δ and the intensity I (since the intensity is entered in %, the value is multiplied by 100) averaged over the total number of assignments K. This results in the following equation MF=1/K·Σ(100·Δ/I %) ( FIG. 2 ). The three parameters, the match factor MF, the number of assigned fragment ions K and sum of non-covered positions M are summarized by the following equation FS=a·MF−b·K+c·M to describe the fitness of the sequence. The value for fitness becomes smaller with smaller mass differences, higher signal intensities, a larger number of assignments and lower sum of non-covered positions. As  FIG. 4  clearly shows, the sequence can only be correctly verified by the interplay of the three individual terms of the equation (match factor MF, number of assigned fragment ions K and sum of non-covered positions M) ( FIG. 4   d ). In contrast other sequences would have been assigned in each case as having a better fit with the MS/MS spectrum if the three individual terms were taken into consideration alone ( FIG. 4   a - c ). Three coefficients a, b and c which weigh the terms of the equation were introduced to combine the match factor MF with the number of assigned fragment ions K and the sum of non-covered positions M. The value for the coefficient a is chosen as 1 by definition, whereas b and c are determined empirically. The coefficient b is selected in such a way that the ratio of the first and second terms of the equation MF:(b·K) is 1:1.3. Thus b=MF/K·1.3 (step  4 ,  FIG. 2 ). A value of 0.1 for the coefficient c has been found to be appropriate for all examples described here. If the coefficients are inserted in the above equation one obtains for FS (step  5 ,  FIG. 2 ): 
   
     
       
         
           FS 
           = 
           
             
               
                 1 
                 / 
                 K 
               
               · 
               Σ 
             
             | 
             
               100 
               · 
               
                 Δ 
                 
                   I 
                   ⁢ 
                   
                       
                   
                   ⁢ 
                   % 
                 
               
             
             | 
             
               
                 
                   - 
                   b 
                 
                 · 
                 K 
               
               + 
               
                 0.1 
                 · 
                 M 
               
             
           
         
       
     
   
   The first calculated value for fitness is a measure of the correspondence of the measured MS/MS spectrum with the set of fragment ions calculated on the basis of the reference sequence. In order to find a sequence that may have a better correspondence or to confirm the identity of the measured sequence with the reference sequence, the reference sequence is systematically varied at every position and subsequently a new fitness is calculated for each of these mutated sequences. This is done by sequentially replacing the base at each position by the three other bases while the remainder of the sequence is kept constant (step  6   a,    FIG. 2 ). Assuming that the sequence contains only one mutation, 3n calculations of FS are carried out for a sequence of n nucleotides (step  6   b - e,    FIG. 2 ). In this connection it is important to note that the weight factor b is kept constant for all varied sequences and corresponds to the value (b=MF/K·1.3) calculated in step  4  of the algorithm from the number of assigned fragment ions K and the sum of non-covered positions (M) of the original reference sequence. The result of the overall calculation is a matrix which includes the fitness of the reference sequence (numbers printed in bold type,  FIG. 2 ) and of the varied sequences at the various positions of the sequence ( FIG. 2 ). For example the FS(T) value in line  1  of the matrix (−5.53) represents the fitness of the sequence TGGC. In contrast FS(A) is the value for the sequence in which the T at position 1 was replaced by an A (15.93). It can be seen that in this example the lowest FS value was obtained for the reference sequence leading to the conclusion that the best correspondence is between the experimental spectrum and the reference sequence. Substitution of a base would be indicated by an FS value for a mutated sequence that is smaller than the FS value of the reference sequence. 
   EXAMPLES 
   Example 1 
   Comparative Sequencing of a 20 mer oligodeoxynucleotide 
   The MS/MS spectrum of a 20 mer having the sequence GACAGGAAAG ACTTTCTGGC (Seq. ID No.: 1) is shown in  FIG. 5 . The experiment was carried out under the following conditions: column: monolithic poly (styrene/divinylbenzene), 60×0.2 mm i.d.; mobile phase: (A) 25 mM TEAB, pH 8.4, (B) 25 mM TEAB, pH 8.4, 20% acetonitrile; linear gradient: 5-50% B in 10 min; flow rate: 3.0 μl/min; temperature: 50° C.; scan: 420-2000 amu; electrospray voltage: 3.40 kV; sheath gas: 40 units; sheath liquid: acetonitrile, 3.0 μl/min; product ions of m/z 1542.5 (charge state: 4-); 4.0 amu isolation width; 35% relative collision energy; sample: 222 ng of the 20 mer. The mass spectroscopic data (m/z, I %) obtained in this manner are then used as an input for the comparative sequencing algorithm. The sequence GACAGGAAAG ACATTCTGGC (Seq. ID No.: 2) which contained an A instead of a T at position 13 was used as the reference sequence. Table 1 summarizes the m/z values of the a, a-B, w and w-B fragment ions for the charge states 1- to 4- that were calculated on the basis of the fragmentation mechanisms described above. The matrix of the FS values was then calculated as outlined in the previous section. In order to make it easier to visualize the result, the matrix was shown in the form of a diagram in which the FS values of the individual bases represented by the letters A, T, G and C were plotted versus the position in the oligodeoxynucleotide sequence.  FIG. 6a  shows that the reference sequence yielded the best correspondence for the first 6 bases but from position 7 onwards the FS values for other bases had a better match. Especially exchanging T for A resulted in significantly lower values for fitness which strongly indicated an A to T mutation. The exact position of the mutation resulted from the minimum of the FS values clearly identifying an A to T mutation at position 13. The base sequence obtained in this manner (GACAGGAAAGACTTTCTGGC) (Seq. ID No.: 1) completely agrees with the theoretical base sequence (GACAGGAAAG ACTTTCTGGC) (Seq. ID No.: 1). If this sequence is now used as a reference sequence in the algorithm together with the mass spectrometric data from  FIG. 5 , this results in  FIG. 6   b.  As expected the reference sequence results in the smallest FS value over the entire sequence. The diagram obviously confirms the identity of the reference sequence and thus the sequence of the examined oligodeoxynucleotide. These two examples demonstrate that this method can be used to check the identity of an unknown sequence with a reference sequence as well as to detect point mutations in comparison to a known reference sequence. 
   Example 2 
   Sequencing of a 51 mer oligodeoxynucleotide 
   An interesting field of application for comparative sequencing is the detection of sequence variants in genomic DNA segments that have been amplified by means of the polymerase chain reaction (PCR). This amplification reaction consists of three steps that are repeated in cycles in which, after denaturation of the DNA double helix into single strands, two different oligodeoxynucleotides of a certain sequence, the so-called primers, are hybridized to specific, complementary sites in order to be subsequently extended to again form the double strands in an enzyme-catalysed polymerization reaction. The segment of the genomic DNA that is to be amplified is delimited by the two primers. Since PCR primers normally have a length of about 20 deoxynucleotides, an amplified PCR product must have a length of more than 40 base pairs in order to contain relevant sequence information. Hence we selected a 51 mer in order to demonstrate that it is possible to check the identity of relatively long DNA molecules. For this a 51 mer (molecular mass: 15580.0) having the sequence AAACCACATT CTGAGCATAC CCCCAAAAAA TTTCATGCCG AAGCTGTGGT C (Seq. ID No.: 3) was fragmented and measured. The experiment was carried out under the following conditions: column monolithic (poly(styrene/divinylbenzene) 60×0.2 mm i.d.; mobile phase: (A) 25 mM butyldimethyl-ammonium bicarbonate, pH 8.4, (B) 25 mM butyldimethylammonium bicarbonate, pH 8.4, 40% acetonitrile; linear gradient: 5-70% B in 10 min: flow rate: 2.0 μl/min; temperature: 50° C., scan: 470-2000 amu; electrospray voltage: 3.40 kV; sheath gas: 40 units; sheath liquid: acetonitrile, 3.0 μl/min; product ions m/z 1729.7 (charge state: 9-); 4.0 amu isolation width; 17% relative collision energy; sample: 635 ng of the 51 mer. The measured MS/MS spectrum was compared with the reference sequence and unequivocally identified as correct on the basis of the fitness values ( FIG. 7 ). 
   Example 3 
   Investigation of the Sequence of an 80 mer oligodeoxynucleotide 
   Another example which is intended to demonstrate the applicability of the algorithm to identifying long oligonucleotides is shown in  FIG. 8 . In this case the correctness of the sequence of an 80 mer was checked by comparison with the MS/MS spectrum (CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG) (Seq. ID No.: 4). The experiment was carried out under the following conditions: column: monolithic poly(styrene/divinylbenzene) 60×0.2 mm i.d.; mobile phase: (A) 25 mM butyldimethyl-ammonium bicarbonate, pH 8.4, (B) 25 mM butyldimethylammonium bicarbonate, pH 8.4, 40% acetonitrile; linear gradient: 10-70% B in 10 min; flow rate: 2.0 μl/min; temperature: 70° C.; scan: 470-2000 amu; electrospray voltage: 5.0 kV; sheath gas: 100 units; sheath liquid: acetonitrile, 3.0 μl/min; product ions of m/z 1065.4 (charge state: 23-); 4.0 amu isolation width; 17% relative collision energy; sample: 478 ng of the 80 mer. It turned out that the FS value for the reference sequence always had the smallest value compared to all mutated sequences. Thus the identity of the assumed sequence was unequivocally proven ( FIG. 8 ). 
   While the foregoing invention has been described in some detail for purposes of clarity and understanding, it will be clear to one skilled in the art from a reading of this disclosure that various changes in form and detail can be made without departing from the true scope of the invention. For example, all the techniques and apparatus described above can be used in various combinations. All publications, patents, patent applications, and/or other documents cited in this application are incorporated by reference in their entirety for all purposes to the same extent as if each individual publication, patent, patent application, and/or other document were individually indicated to be incorporated by reference for all purposes. 
   
     
       
         
             
           
             
               TABLE 1 
             
           
          
             
                 
             
             
               Calculated m/z values of the charge states 1- to 4- of the a, a-B, w and 
             
             
               w-B fragment ions of the reference sequence 
             
             
               GACAGGAAAGACATTCTGGC (Seq. ID NO.: 2). 
             
          
         
         
             
             
             
          
             
                 
               Charge state 
                 
             
          
         
         
             
             
             
             
             
             
          
             
                 
               Typ 
               −1 
               −2 
               −3 
               −4 
             
             
                 
                 
             
          
         
         
             
             
             
             
             
             
          
             
                 
               a1-B1 
               97.04 
               48.02 
               31.68 
               23.51 
             
             
                 
               w1-B20 
               195.01 
               97.01 
               64.34 
               48.00 
             
             
                 
               a1 
               248.09 
               123.55 
               82.03 
               61.27 
             
             
                 
               w1 
               306.06 
               152.53 
               101.35 
               75.77 
             
             
                 
               a2-B2 
               426.09 
               212.55 
               141.36 
               105.77 
             
             
                 
               w2-B19 
               484.06 
               241.53 
               160.69 
               120.27 
             
             
                 
               a2 
               561.14 
               280.07 
               186.38 
               139.54 
             
             
                 
               w2 
               635.11 
               317.06 
               211.04 
               158.03 
             
             
                 
               a3-B3 
               739.15 
               369.08 
               245.72 
               184.04 
             
             
                 
               w3-B18 
               813.11 
               406.06 
               270.37 
               202.53 
             
             
                 
               a3 
               850.19 
               424.60 
               282.73 
               211.80 
             
             
                 
               w3 
               964.16 
               481.58 
               320.72 
               240.29 
             
             
                 
               a4-B4 
               1028.19 
               513.60 
               342.06 
               256.30 
             
             
                 
               w4-B17 
               1142.16 
               570.58 
               380.05 
               284.79 
             
             
                 
               a4 
               1163.25 
               581.13 
               387.08 
               290.06 
             
             
                 
               w4 
               1268.21 
               633.61 
               422.07 
               316.30 
             
             
                 
               a5-B5 
               1341.25 
               670.13 
               446.42 
               334.56 
             
             
                 
               w5-B16 
               1446.21 
               722.61 
               481.40 
               360.80 
             
             
                 
               a5 
               1492.30 
               745.65 
               496.77 
               372.33 
             
             
                 
               w5 
               1557.25 
               778.13 
               518.42 
               388.56 
             
             
                 
               a6-B6 
               1670.30 
               834.65 
               556.10 
               416.83 
             
             
                 
               w6-B15 
               1735.26 
               867.13 
               577.75 
               433.07 
             
             
                 
               a6 
               1821.35 
               910.18 
               606.45 
               454.59 
             
             
                 
               w6 
               1861.30 
               930.15 
               619.77 
               464.58 
             
             
                 
               a7-B7 
               1999.35 
               999.18 
               665.78 
               499.09 
             
             
                 
               w7-B14 
               2039.30 
               1019.15 
               679.10 
               509.08 
             
             
                 
               a7 
               2134.41 
               1066.71 
               710.80 
               532.85 
             
             
                 
               w7 
               2165.34 
               1082.17 
               721.11 
               540.59 
             
             
                 
               a8-B8 
               2312.41 
               1155.71 
               770.14 
               577.35 
             
             
                 
               w8-B13 
               2343.35 
               1171.18 
               780.45 
               585.09 
             
             
                 
               a8 
               2447.47 
               1223.24 
               815.16 
               611.12 
             
             
                 
               w8 
               2478.40 
               1238.70 
               825.47 
               618.85 
             
             
                 
               a9-B9 
               2625.47 
               1312.24 
               874.49 
               655.62 
             
             
                 
               a9-B12 
               2656.40 
               1327.70 
               884.80 
               663.35 
             
             
                 
               a9 
               2760.52 
               1379.76 
               919.51 
               689.38 
             
             
                 
               w9 
               2767.45 
               1383.23 
               921.82 
               691.11 
             
             
                 
               a10-B10 
               2938.53 
               1468.77 
               978.84 
               733.88 
             
             
                 
               w10-B11 
               2945.45 
               1472.23 
               981.15 
               735.61 
             
             
                 
               w10 
               3080.51 
               1539.76 
               1026.17 
               769.38 
             
             
                 
               a10 
               3089.58 
               1544.29 
               1029.19 
               771.65 
             
             
                 
               w11-B10 
               3258.51 
               1628.76 
               1085.50 
               813.88 
             
             
                 
               a11-B11 
               3267.58 
               1633.29 
               1088.53 
               816.15 
             
             
                 
               a11 
               3402.63 
               1700.82 
               1133.54 
               849.91 
             
             
                 
               w11 
               3409.56 
               1704.28 
               1135.85 
               851.64 
             
             
                 
               a12-B12 
               3580.64 
               1789.82 
               1192.88 
               894.41 
             
             
                 
               w12-B9 
               3587.56 
               1793.28 
               1195.19 
               896.14 
             
             
                 
               a12 
               3691.68 
               1845.34 
               1229.89 
               922.17 
             
             
                 
               w12 
               3722.62 
               1860.81 
               1240.21 
               929.91 
             
             
                 
               a13-B13 
               3869.68 
               1934.34 
               1289.23 
               966.67 
             
             
                 
               w13-B8 
               3900.62 
               1949.81 
               1299.54 
               974.41 
             
             
                 
               a13 
               4004.74 
               2001.87 
               1334.25 
               1000.44 
             
             
                 
               w13 
               4035.67 
               2017.34 
               1344.56 
               1008.17 
             
             
                 
               a14-B14 
               4182.74 
               2090.87 
               1393.58 
               1044.94 
             
             
                 
               w14-B7 
               4213.68 
               2106.34 
               1403.89 
               1052.67 
             
             
                 
               a14 
               4308.78 
               2153.89 
               1435.59 
               1076.45 
             
             
                 
               w14 
               4348.73 
               2173.87 
               1448.91 
               1086.43 
             
             
                 
               a15-B15 
               4486.79 
               2242.90 
               1494.93 
               1120.95 
             
             
                 
               w15-B6 
               4526.73 
               2262.87 
               1508.24 
               1130.93 
             
             
                 
               a15 
               4612.83 
               2305.92 
               1536.94 
               1152.46 
             
             
                 
               w15 
               4677.78 
               2338.39 
               1558.59 
               1168.70 
             
             
                 
               a16-B16 
               4790.83 
               2394.92 
               1596.28 
               1196.96 
             
             
                 
               w16-B5 
               4855.79 
               2427.40 
               1617.93 
               1213.20 
             
             
                 
               a16 
               4901.87 
               2450.44 
               1633.29 
               1224.72 
             
             
                 
               w16 
               5006.83 
               2502.92 
               1668.28 
               1250.96 
             
             
                 
               a17-B17 
               5079.88 
               2539.44 
               1692.63 
               1269.22 
             
             
                 
               w17-B4 
               5184.84 
               2591.92 
               1727.61 
               1295.46 
             
             
                 
               a17 
               5205.92 
               2602.46 
               1734.64 
               1300.73 
             
             
                 
               w17 
               5319.89 
               2659.45 
               1772.63 
               1329.22 
             
             
                 
               a18-B18 
               5383.92 
               2691.46 
               1793.97 
               1345.23 
             
             
                 
               w18-B3 
               5497.89 
               2748.45 
               1831.96 
               1373.72 
             
             
                 
               a18 
               5534.97 
               2766.99 
               1844.32 
               1382.99 
             
             
                 
               w18 
               5608.94 
               2803.97 
               1868.98 
               1401.89 
             
             
                 
               a19-B19 
               5712.98 
               2855.99 
               1903.66 
               1427.50 
             
             
                 
               w19-B2 
               5786.94 
               2892.97 
               1928.31 
               1445.99 
             
             
                 
               a19 
               5864.02 
               2931.51 
               1954.01 
               1465.26 
             
             
                 
               w19 
               5922.00 
               2960.50 
               1973.33 
               1479.75 
             
             
                 
               [M] 
               6171.08 
               3085.04 
               2056.36 
               1542.02