Patent Publication Number: US-2005143403-A1

Title: Substituted pyrimidinones and pyrimidinthiones

Description:
FIELD OF THE INVENTION  
      The present invention relates generally to compounds that bind to CRF receptors, and particularly to substituted pyrimidinone and pyrimidinthione derivatives as CRF 1  receptor antagonists and to the use thereof as a treatment for disorders that are associated with CRF or CRF 1  receptors.  
     BACKGROUND OF THE INVENTION  
      Corticotropin releasing factor (CRF) is a 41 amino acid peptide that is the primary physiological regulator of proopiomelanocortin (POMC) derived peptide secretion from the anterior pituitary gland [J. Rivier et al.,  Proc. Natl. Acad. Sci  ( USA ) 80: 4851 (1983); W. Vale et al.,  Science  213: 1394 (1981)]. In addition to its endocrine role at the pituitary gland, CRF is known to have a broad extrahypothalmic distribution in the CNS, contributing therein to a wide spectrum of autonomic behavioral and physiological effects consistent with a neurotransmitter or neuromodulator role in the brain [W. Vale et al.,  Rec. Prog. Horm. Res.  39: 245 (1983); G. F. Koob,  Persp. Behav. Med.  2: 39 (1985); E. B. De Souza et al.,  J. Neurosci.  5: 3189 (1985)]. There is evidence that CRF plays a significant role in integrating the response in the immune system to physiological, psychological, and immunological stressors, in psychiatric disorders and neurological diseases including depression, anxiety-related disorders and feeding disorders, and in the etiology and pathophysiology of Alzheimer&#39;s disease, Parkinson&#39;s disease, Huntington&#39;s disease, progressive supranuclear palsy and amyotrophic lateral sclerosis, as they relate to the dysfunction of CRF neurons in the central nervous system [J. E. Blalock,  Physiological Reviews  69: 1 (1989); J. E. Morley,  Life Sci.  41: 527 (1987); E. B. De Souze,  Hosp. Practice  23: 59 (1988)].  
      It was shown that in individuals afflicted with affective disorder, or major depression, the concentration of CRF in the cerebral spinal fluid (CSF) is significantly increased. [C. B. Nemeroff et al.,  Science  226: 1342 (1984); C. M. Banki et al.,  Am. J. Psychiatry  144: 873 (1987); R. D. France et al.,  Biol. Psychiatry  28: 86 (1988); M. Arato et al.,  Biol. Psychiatry  25: 355 (1989)]. Furthermore, the density of CRF receptors is significantly decreased in the frontal cortex of suicide victims, consistent with a hypersecretion of CRF [C. B. Memeroff et al.,  Arch. Gen. Psychiatry  45: 577 (1988)]. In addition, there is a blunted adrenocorticotropin (ACTH) response to CRF (i.v. administered) observed in depressed patients [P. W. Gold et al.,  Am. J. Psychiatry  141: 619 (1984); F. Holsboer et al.,  Psychoneuroendocrinology  9: 147 (1984); P. W. Gold et al.,  New Engl. J. Med.  314: 1129 (1986)]. Preclinical studies in rats and non-human primates provide additional support for the hypothesis that hypersecretion of CRF may be involved in the symptoms seen in human depression [R. M. Sapolsky,  Arch. Gen. Psychiatry  46: 1047 (1989)]. There is also preliminary evidence that tricyclic antidepressants can alter CRF levels and thus modulate the numbers of receptors in the brain [Grigoriadis et al.,  Neuropsychopharmacology  2: 53 (1989)].  
      CRF has also been implicated in the etiology of anxiety-related disorders. Anxiety disorders are a group of diseases, recognized in the art, that includes phobic disorders, anxiety states, post-traumatic stress disorder and atypical anxiety disorders [The Merck Manual of Diagnosis and Therapy, 16 th  edition (1992)]. Emotional stress is often a precipitating factor in anxiety disorders, and such disorders generally respond to medications that lower response to stress. Excessive levels of CRF are known to produce anxiogenic effects in animal models [see, e.g., Britton et al., 1982; Berridge and Dunn, 1986 and 1987]. Interactions between benzodiazepine/non-benzodiazepine anxiolytics and CRF have been demonstrated in a variety of behavioral anxiety models [D. R. Britton et al.,  Life Sci.  31: 363 (1982); C. W. Berridge and A. J. Dunn,  Regul. Peptides  16: 83 (1986)]. Studies using the putative CRF receptor antagonist α-helical ovine CRF (9-41) in a variety of behavioral paradigms demonstrates that the antagonist produces “anxiolytic-like” effects that are qualitatively similar to the benzodiazepines [C. W. Berridge and A. J. Dunn,  Horm. Behav.  21: 393 (1987),  Brain Research Reviews  15: 71 (1990); G. F. Koob and K. T. Britton, In:  Corticotropin - Releasing Factor: Basic and Clinical Studies of a Neuropeptide , E. B. De Souza and C. B. Nemeroff eds., CRC Press p. 221 (1990)]. Neurochemical, endocrine and receptor binding studies have all demonstrated interactions between CRF and benzodiazepine anxiolytics, providing further evidence for the involvement of CRF in these disorders. Chlordiazepoxide attenuates the “anxiogenic” effects of CRF both in the conflict test [K. T. Britton et al.,  Psychopharmacology  86: 170 (1985); K. T. Britton et al.,  Psychopharmacology  94: 306 (1988)] and in the acoustic startle test [N. R. Swerdlow et al.,  Psychopharmacology  88: 147 (1986)] in rats. The benzodiazepine receptor antagonist Ro 15-1788, which was without behavioral activity alone in the operant conflict test, reversed the effects of CRF in a dose-dependent manner while the benzodiazepine inverse agonist FG 7142 enhanced the actions of CRF [K. T. Britton et al.,  Psychopharmacology  94: 396 (1988)].  
      The use of CRF 1  antagonists for the treatment of Syndrome X has also been described in U.S. patent application Ser. No. 09/696,822, filed Oct. 26, 2000, and European Patent Application No. 003094414, filed Oct. 26, 2000. Methods for using CRF 1  antagonists to treat congestive heart failure are described in U.S. Ser. No. 09/248,073, filed Feb. 10, 1999, now U.S. Pat. No. 6,043,260 (Mar. 28, 2000).  
      It has also been suggested that CRF 1  antagonists are useful for treating arthritis and inflammation disorders [E. L. Webster et al.,  J Rheumatol  29: 1252 (2002); E. P. Murphy et al.,  Arthritis Rheum  44: 782 (2001)]; stress-related gastrointestinal disorders [K. E. Gabry et al.,  Molecular Psychiatry  7: 474 (2002)]; and skin disorders [C. C. Zouboulis et al.,  Proc. Natl. Acad. Sci.  99: 7148 (2002)].  
      It was disclosed recently that, in an animal model, stress-induced exacerbation of chronic contact dermatitis is blocked by a selective CRF 1  antagonist, suggesting that that CRF 1  is involved in the stress-induced exacerbation of chronic contact dermatitis and that CRF 1  antagonist may be useful for treating this disorder. [K. Kaneko et al.,  Exp Dermatol,  12: 47 (2003).  
      WO 02/06242 and WO 01/68614 disclose various compounds that can bind with high affinity and high selectivity to CRF 1  receptors. The compounds are useful for treating CNS-related disorders particularly affective disorders and diseases, and acute and chronic neurological disorders and diseases.  
      It is an object of the invention to provide novel pyrimidinone and pyrimidithione derivatives, which are CRF 1  receptor antagonists.  
      It is another object of the invention to provide novel compounds as treatment of disorders or conditions that are associated with CRF or CRF 1  receptors, such as anxiety disorders, depression, and stress related disorders.  
      It is another object of the invention to provide a method of treating disorders or conditions that are associated with CRF or CRF 1  receptors, such as anxiety disorders, depression, and stress related disorders.  
      It is yet another object of the invention to provide a pharmaceutical composition useful for treating disorders or conditions that are associated with CRF or CRF 1  receptors, such as anxiety disorders, depression, and stress related disorders.  
      There are other objects of the invention which will be evident or apparent from the description of the invention in the specification of the application.  
     SUMMARY OF THE INVENTION  
      The present invention provides a compound of Formula I,  
                 
 
 a stereoisomer thereof, a pharmaceutically acceptable salt thereof, or a prodrug thereof, or a pharmaceutically acceptable salt of a prodrug thereof, wherein: 
          X is selected from NR 3 R 4 , OR 3 , CR 3 R 5 R 5 , C(O)R 3 , S(O) m R 3 , NR 3 C(O)R 4 , NR 3 S(O) m R 4 , cycloalkyl, aryl, heterocycloalkyl, heteroaryl, aryl cycloalkyl, substituted aryl cycloalkyl, heteroaryl cycloalkyl, substituted heteroaryl cycloalkyl, aryl heterocycloalkyl, substituted aryl heterocycloalkyl, heteroaryl heterocycloalkyl, or substituted heteroaryl heterocycloalkyl (point of attachment being either nitrogen or carbon);     Z is selected from —O, —S, and —NR 2 ;     m is 0, 1, or 2;     Ar is selected from aryl, substituted aryl, heteroaryl, substituted heteroaryl; and G;     G is selected from  
                 
 
 wherein each G group may have from 1 to 4 substituents independently selected from halogen, alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, —OR 5 , SR 5 , —NR 5 R 5 , —C(O)R 5 , —C(S)R 5 , —CN, —C(O)NR 5 R 5 , —C(S)NR 5 R 5 , —NR 5 C(O)R 5 , —NR 5 C(S)R 5 , —S(O) 2 NR 5 R 5 , —NR 5 S(O) 2 R 5 , —NO 2 , aryl, substituted aryl, heteroaryl, substituted heteroaryl; 
    n is 0, 1, 2, or 3;     R 1  is selected from halogen, —NO 2 , —CN, —R a , —OR a , —S(O) m R a , —NR a R a , —C(O)NR a R a , —C(S)NR a R a  —S(O) m NR a R a , —NR a S(O) m R a , —NR a C(O)OR a , —NR a C(S)OR a , —OC(O)NR a R a , —OC(S)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)OR a , or —CR a R a Ar;     R 2  is selected from —R a , —S(O) m R a , —C(O)NR a R a , —C(S)NR a R a  —S(O) m NR a R a , —C(O)OR a , or —C(S)OR a ;     R 3 , R 4  and R 5  are independently selected from R a , substituted alkyl, heterocycloalkyl, substituted heterocycloalkyl, substituted heteroaryl, substituted aryl, aryl cycloalkyl, substituted aryl cycloalkyl, heteroaryl cycloalkyl, substituted heteroaryl cycloalkyl, aryl heterocycloalkyl, substituted aryl heterocycloalkyl, heteroaryl heterocycloalkyl, or substituted heteroaryl heterocycloalkyl, wherein R 3  and R 4 , when both present, may together form a monocyclic or bicyclic ring (saturated or unsaturated) optionally substituted with a R s .        

      R s  each is independently selected from substituted alkyl, OR a , —NO 2 , —C(O)NR a R a , —C(S)NR a R a  —S(O) m NR a R a , —NR a S(O) m R a , —NR a R a C(O)OR a , —NR a C(S)OR a , —OC(O)NR a R a , —OC(S)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)R a , —OC(S)R a , or —OC(O)OR a ; 
          R a  each is selected from H, alkyl, cycloalkyl, haloalkyl, aryl, heteroaryl, or heterocycloalkyl, where each instance of R a  may be optionally substituted with 1 to 5 of R t , —OR t , —S(O) m R t , —NR t R t , oxo (═O), thione (═S); and     R t  each is selected from H, halogen, —NO 2 , —NH 2 , —OH, —SH, —CN, —C(O)NH 2 , —C(S)NH 2 , —C(O)—NHalkyl, —C(S)—NHalkyl, —C(O)Nalkylalkyl, —C(S)Nalkylalkyl, —Oalkyl, NHalkyl, Nalkylalkyl, —S(O) m alkyl, SO 2 NH 2 , SO 2 NHalkyl, SO 2 Nalkylalkyl, alkyl, cycloalkyl, haloalkyl, phenyl, benzyl, heteroaryl, or heterocycloalkyl, where phenyl, benzyl, heteroaryl, and heterocycloalkyl may be optionally substituted with alkyl or halogen.        

      In another aspect, the present invention provides a pharmaceutical composition comprising a compound of Formula I, a stereoisomer thereof, a pharmaceutically acceptable salt thereof, or a prodrug thereof, or a pharmaceutically acceptable salt of the prodrug thereof. The compositions can be prepared in any suitable forms such as tablets, pills, powders, lozenges, sachets, cachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols, and ointments.  
      The compounds of the inventions are CRF 1  receptor antagonists and are useful for treating disorders or conditions associated with CRF or CRF 1  receptors, including human CRF 1  receptors.  
      Thus, in another aspect, the present invention provides a method of antagonizing CRF 1  receptors in a warm-blooded animal, comprising administering to the animal a compound of the invention at amount effective to antagonize CRF 1  receptors.  
      In still another aspect, the present invention provides a method for screening for ligands for CRF 1  receptors, which method comprises: a) carrying out a competitive binding assay with CRF 1  receptors, a compound of Formula I which is labeled with a detectable label, and a candidate ligand; and b) determining the ability of said candidate ligand to displace said labeled compound.  
      In still another aspect, the present invention provides a method for detecting CRF, receptors in a tissue comprising: a) contacting a compound of Formula I, which is labeled with a detectable label, with a tissue, under conditions that permit binding of the compound to the tissue; and b) detecting the labeled compound bound to the tissue.  
      In yet another aspect, the present invention provides a method of inhibiting the binding of CRF to CRF 1  receptors in vitro, comprising contacting a compound of the invention with a solution comprising cells expressing the CRF 1  receptor, such as IMR32 cells, wherein the compound is present in the solution at a concentration sufficient to inhibit the binding of CRF to the CRF 1  receptor.  
      Compounds of the invention are useful for treating, in a warm-blooded animal, particularly a mammal, and more particularly a human, various disorders that are associated with CRF or CRF 1  receptors, or disorders the treatment of which can be effected or facilitated by antagonizing CRF 1  receptors. Examples of such disorders include anxiety-related disorders (such as anxiety, generalized anxiety disorder, social anxiety disorder, anxiety with co-morbid depressive illness, panic disorder, and obsessive-compulsive disorder); mood disorders (such as depression, including major depression, single episode depression, recurrent depression, child abuse induced depression, and postpartum depression, and affective disorder); post-traumatic stress disorder; supranuclear palsy; immune suppression; drug or alcohol withdrawal symptoms; substance abuse disorder (e.g., nicotine, cocaine, ethanol, opiates, or other drugs); inflammatory disorders (such as rheumatoid arthritis and osteoarthritis); fertility problems including infertility; pain; asthma; psoriasis and allergies; phobias; sleep disorders induced by stress; pain perception (such as fibromyalgia); dysthemia; bipolar disorders; cyclothymia; fatigue syndrome; stress-induced headache; cancer; human immunodeficiency virus (HIV) infections; neurodegenerative diseases (such as Alzheimer&#39;s disease, Parkinson&#39;s disease and Huntington&#39;s disease); gastrointestinal diseases (such as ulcers, irritable bowel syndrome, Crohn&#39;s disease, spastic colon, diarrhea, and post operative ilius and colonic hypersensitivity associated by psychopathological disturbances or stress); eating disorders (such as anorexia and bulimia nervosa and other feeding disorders); hemorrhagic stress; stress-induced psychotic episodes; euthyroid sick syndrome; syndrome of inappropriate antidiarrhetic hormone (ADH); obesity; head traumas; spinal cord trauma; ischemic neuronal damage (e.g., cerebral ischemia such as cerebral hippocampal ischemia); excitotoxic neuronal damage; epilepsy; cardiovascular and heart related disorders (such as hypertension, tachycardia and congestive heart failure); stroke; immune dysfunctions including stress induced immune dysfunctions (e.g., stress induced fevers, porcine stress syndrome, bovine shipping fever, equine paroxysmal fibrillation, and dysfunctions induced by confinement in chickens, sheering stress in sheep or human-animal interaction related stress in dogs); muscular spasms; urinary incontinence; senile dementia of the Alzheimer&#39;s type; multiinfarct dementia; amyotrophic lateral sclerosis; chemical dependencies and addictions (e.g., dependences on alcohol, cocaine, heroin, benzodiazepines, or other drugs); osteoporosis; psychosocial dwarfism, hypoglycemia, and skin disorders (such as acne, psoriasis, chronic contact demertitis, and stress-exacerbated skin disorders). They are also useful for promoting smoking cessation and hair growth, or treating hair loss.  
      Thus, in yet a further aspect the present invention provides a method of treating a disorder, in warm-blooded animal, the treatment of which disorder can be effected or facilitated by antagonizing CRF 1  receptors, which method comprises administration to a patient in need thereof an effective amount of a compound of Formula I. In a particular embodiment the invention provides a method for the treatment of disorders that manifest hypersecretion of CRF. Examples of disorders that can be treated with the compounds of the invention include generalized anxiety disorder; social anxiety disorder; anxiety; obsessive-compulsive disorder; anxiety with co-morbid depressive illness; panic disorder; and mood disorders such as depression, including major depression, single episode depression, recurrent depression, child abuse induced depression, postpartum depression, hair loss, and contact demertitis. It is preferred that the warm-blooded animal is a mammal, and more preferred that the animal is a human. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION  
      The present invention provides a compound of Formula I described above.  
      Preferred compounds of formula I include compounds of Formula II.  
                 
 
      Preferred compounds of formula II include compounds of Formula III  
                 
 
 wherein in Formula III, p is 1, 2, 3 or 4. 
 
      Preferred compounds of Formula III include compounds of Formula IV  
                 
 
 wherein in Formula IV, q is 0, 1, 2, 3, or 4; 
 
      Preferred compounds of Formula III further include compounds of Formula V  
                 
 
 wherein in Formula V, r is 0, 1, 2, 3, 4 or 5; R w  each is independently selected from substituted alkyl, OR a , —NO 2 , —C(S)NR a R a  —S(O) m NR a R a , —NR a S(O) m R a , —NR a C(O)OR a , —NR a C(S)OR a , —OC(O)NR a R a , —OC(S)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)R a , —OC(S)R a , or —OC(O)OR a ; 
 
      Following are examples of particular compounds of the invention, with each compound being identified by both a chemical name and a structural formula immediately below the chemical name: 
      2-(2,4-Dichlorophenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-dimethylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-methoxyphenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-dimethylpyrimidin-4(3H)-one  
                 
    2-(2-Methyl-4-methoxyphenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-dimethylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-dimethylaminophenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-dimethylpyrimidin-4(3H)-one  
                 
    2-[6-(Dimethylamino)-4-methylpyridin-3-yl]-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-dimethylpyrimidin-4(3H)-one  
                 
    5-[(2S,4R)-4-Methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-2-(6-methoxy-2-methylpyridin-3-yl)-3,6-dimethylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-trifluoromethylphenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-dimethylpyrimidin-4(3H)-one  
                 
    2-(2-Trifluoromethyl-4-dimethylaminophenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-dimethylpyrimidin-4(3H)-one  
                 
    2-(2,4-Dichlorophenyl)-6-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3-methylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-methoxyphenyl)-6-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3-methylpyrimidin-4(3H)-one  
                 
    2-(2-Methyl-4-methoxyphenyl)-6-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3-methylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-dimethylaminophenyl)-6-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3-methylpyrimidin-4(3H)-one  
                 
    2-[6-(Dimethylamino)-4-methylpyridin-3-yl]-6-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrol idin-1-yl]-3-methylpyrimidin-4(3H)-one  
                 
    6-Ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrol idin-1-yl]-2-(6-methoxy-2-methylpyridin-3-yl)-3-methylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-trifluoromethylphenyl)-6-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3-methylpyrimidin-4(3H)-one  
                 
    2-(2-Trifluoromethyl-4-dimethylaminophenyl)-6-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3-methylpyrimidin-4(3H)-one  
                 
    2-(2,4-Dichlorophenyl)-3-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-6-methylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-methoxyphenyl)-3-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-6-methylpyrimidin-4(3H)-one  
                 
    2-(2-Methyl-4-methoxyphenyl)-3-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-6-methylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-dimethylaminophenyl)-3-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-6-methylpyrimidin-4(3H)-one  
                 
    2-[6-(Dimethylamino)-4-methylpyridin-3-yl]-3-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-6-methylpyrimidin-4(3H)-one  
                 
    3-Ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-2-(6-methoxy-2-methylpyridin-3-yl)-6-methylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-trifluoromethylphenyl)-3-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-6-methylpyrimidin-4(3H)-one  
                 
    2-(2-Trifluoromethyl-4-dimethylaminophenyl)-3-ethyl-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-6-methylpyrimidin-4(3H)-one  
                 
    2-(2,4-Dichlorophenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-diethylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-methoxyphenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-diethylpyrimidin-4(3H)-one  
                 
    2-(2-Methyl-4-methoxyphenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-diethylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-dimethylaminophenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-diethylpyrimidin-4(3H)-one  
                 
    2-[6-(Dimethylamino)-4-methylpyridin-3-yl]-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-diethylpyrimidin-4(3H)-one  
                 
    5-[(2S,4R)-4-Methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-2-(6-methoxy-2-methylpyridin-3-yl)-3,6-diethylpyrimidin-4(3H)-one  
                 
    2-(2-Chloro-4-trifluoromethylphenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-diethylpyrimidin-4(3H)-one  
                 
    2-(2-Trifluoromethyl-4-dimethylaminophenyl)-5-[(2S,4R)-4-methoxy-2-(methoxymethyl)pyrrolidin-1-yl]-3,6-diethylpyrimidin-4(3H)-one  
                 
   

      Compounds of the invention can be prepared using the synthetic routes illustrated in Charts A-E indicated below. Starting materials are either commercially available or can be prepared by procedures known to one of ordinary skill in organic chemistry.  
                 
 
      In Chart A, Q=bromo, iodo, or chloro and R 3  and R 4  are as defined for Formula I. Compound such as A-1 can be prepared according to literature procedures (T. L. Cupps et al.,  J. Org. Chem.  1983, 48, 1060). Intermediate A-1 can be converted into A-2 by a transition metal-catalyzed coupling with a metalloaryl reagent (Ar-[M]). Commonly used reagent/catalyst pairs include aryl boronic acid/palladium(0) (N. Miyaura and A. Suzuki,  Chem. Rev.  1995, 95, 2457), aryl trialkylstannane/palladium(0) (T. N. Mitchell,  Synthesis  1992, 803), arylzinc/palladium(0) and aryl Grignard/nickel(II). Palladium(0) represents a catalytic system made of various combinations of metal/ligand pair which includes, but not limited to, tetrakis(triphenylphosphine)palladium(0), palladium(II)acetate/tri(o-tolyl)phosphine, tris(dibenzylideneacetone)dipalladium(0)/tri-tert-butylphosphine, and dichloro[1,1′-bis(diphenylphosphine)ferrocene]palladium(0). Nickel(II) represents a nickel-containing catalyst such as [1,2-bis(diphenylphosphino)ethane]dichloronickel(II) and [1,3-bis(diphenylphosphino)propane]dichloronickel(II). The nitro group present in A-2 may be reduced by a variety of methods known in the art, including hydrogenation with hydrogen and transition metal catalysts or the use of sodium hydrosulfite in aqueous solutions or the use of Zn/CaCl 2  in aqueous ethanol to afford A-3. A-3 may be transformed into A-4 by reductive amination using aldehydes or ketones and reducing agents such as sodium triacetoxyborohydride in inert solvents. The order of the steps in Chart A may be altered depending upon the substitution of the aromatic (Ar) group. For instance, for disubstituted aromatic analogs, A-1 may first be coupled with a boronic acid, the nitro group reduced and the resulting amine alkylated to give compouns of generic structure A-4. Conversion of A-4 into A-5 may be carried out by a number of methods known in the art, including for example the use of hydrochloric acid, boron trichloride, boron tribromide, acetic acid, trimethylsilyl bromide, trimethylsilyl chloride, or aluminum tribromide, in a solvent such as dichloromethane or DMF. N-Alkylation of A-5 to the target compound A-6 may be accomplished using a base such as but not limited to alkali metal hydride or alkali metal alkoxide in inert solvents such as but not limited to THF, DMF, or methyl sulfoxide. Alkylation may be conducted using alkyl halide, suitably bromide, iodide, tosylate or mesylate at temperatures ranging from −78° C. to 100° C.  
                 
 
      The alkylation of the methyl group (or other alkyl group) on position 6 of the pyrimidine (e.g., B-1) may be accomplished using a strong base such as but not limited to alkali metal hydride, alkali metal amide or alkali metal alkoxide in inert solvents such as but not limited to THF, DMF, or methyl sulfoxide. Alkylation may be conducted using alkyl halide, suitably bromide, iodide, tosylate or mesylate at temperatures ranging from −78° C. to 100° C. Using the same methods described in Chart A, compounds of formula B-4 can also be prepared as outlined in Chart B.  
                 
 
      A route for preparing pyrimidinthiones is outlined in Chart C. Compound C-1 can be prepared by the methods outlined in Charts A-B. Using methods well known in the literature, C-1 can be converted into the target compound C-2 using reagents such as but not limited to P 2 S 5  or Lawesson&#39;s reagent (see K. Woerner, et al.,  Helv. Chim. Acta  1999, 82, 2094).  
                 
 
      A route for preparing iminopyrimidines is shown in Chart D. Compound D-1 can be prepared by the methods outlined in Charts A-B. Using methods well known in the literature, D-1 can be converted into the target compound D-2 using reagents such as but not limited to POCl 3 . Treating D-2 with amines in suitable solvents such as but not limited to ethanol or toluene affords D-3 (see D. M. Brown, P. K. T. Lin,  Carbohydr. Res.  1991, 216, 129; H. Paulsen, U. Maass,  Chem. Ber.  1981, 114, 346-351). Using the same methods outlined in Chart A, compounds of the Formula D-4 can also be prepared as outlined in Chart D.  
      The compounds of formula I can also be prepared as illustrated in Chart E as E-5. Compound E-1 can be prepared by the methods described in Chart A. Treating E-1 with (butyl)nitrite and copper halide (for example CuBr) affords the halogen (I, Br, Cl) compounds E-2. Following the protocol of Buchwald et al ( J. Org. Chem.  2000, 1158), the desired amines E-3 can be produced. Using the same methods outlined in Chart A, compounds of the formula E-5 can also be prepared as outlined in Chart E.  
                 
 
      It should be understood that compounds provided herein can have one or more asymmetric centers or planes, and all chiral (enantiomeric and diastereomeric) and racemic forms of the compound are included in the present invention. Many geometric isomers of olefins, C═N double bonds, and the like can also be present in the compounds, and all such stable isomers are contemplated in the present invention. Compounds of the invention are isolated in either the racemic form, or in the optically pure form, for example, by resolution of the racemic form by conventional methods such as crystallization in the presence of a resolving agent, or chromatography, using, for example, a chiral HPLC column, or synthesized by a asymmetric synthesis route enabling the preparation of enantiomerically enriched material. The present invention encompasses all possible tautomers of the compounds represented by Formula I.  
      The present invention also encompasses pharmaceutically acceptable salts of compounds of Formula I. Examples of pharmaceutically acceptable salts are salts prepared from inorganic acids or organic acids, such as inorganic and organic acids of basic residues such as amines, for example, acetic, benzenesulfonic, benzoic, amphorsulfonic, citric, ethenesulfonic, fumaric, gluconic, glutamic, hydrobromic, hydrochloric, isethionic, lactic, maleic, malic, mandelic, methanesulfonic, mucic, nitric, pamoic, pantothenic, phosphoric, succinic, sulfuric, barbaric acid, p-toluenesulfonic and the like; and alkali or organic salts of acidic residues such as carboxylic acids, for example, alkali and alkaline earth metal salts derived from the following bases: sodium hydride, sodium hydroxide, potassium hydroxide, calcium hydroxide, aluminum hydroxide, lithium hydroxide, magnesium hydroxide, zinc hydroxide, ammonia, trimethylammonia, triethylammonia, ethylenediamine, lysine, arginine, ornithine, choline, N,N′-dibenzylethylenediamine, chloroprocaine, diethanolamine, procaine, n-benzylphenethylamine, diethylamine, piperazine, tris(hydroxymethyl)-aminomethane, tetramethylammonium hydroxide, and the like.  
      Pharmaceutically acceptable salts of the compounds of the invention can be prepared by conventional chemical methods. Generally, such salts are, for example, prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, non-aqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred. Lists of suitable salts are found in Remington&#39;s Pharmaceutical Sciences, 17 th  ed., Mack Publishing Company, Easton, Pa., 1985, p. 1418, the disclosure of which is hereby incorporated by reference.  
      In another aspect, the present invention provides a prodrug of a compound of Formula I. The prodrug is prepared with the objective(s) of improved chemical stability, improved patient acceptance and compliance, improved bioavailability, prolonged duration of action, improved organ selectivity (including improved brain penetrance), improved formulation (e.g., increased hydrosolubility), and/or decreased side effects (e.g., toxicity). See e.g. T. Higuchi and V. Stella, “Prodrugs as Novel Delivery Systems”, Vol. 14 of the A.C.S.  
      Symposium Series; Bioreversible Carriers in Drug Design, ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, (1987). Prodrugs include, but are not limited to, compounds derived from compounds of Formula I wherein hydroxy, amine or sulfhydryl groups, if present, are bonded to any group that, when administered to the subject, cleaves to form the free hydroxyl, amino or sulfhydryl group, respectively. Selected examples include, but are not limited to, biohydrolyzable amides and biohydrolyzable esters and biohydrolyzable carbamates, carbonates, acetate, formate and benzoate derivatives of alcohol and amine functional groups.  
      The prodrug can be readily prepared from the compounds of Formula I using methods known in the art. See, e.g. See Notari, R. E., “Theory and Practice of Prodrug Kinetics,” Methods in Enzymology, 112: 309-323 (1985); Bodor, N., “Novel Approaches in Prodrug Design,” Drugs of the Future, 6(3): 165-182 (1981); and Bundgaard, H., “Design of Prodrugs: Bioreversible-Derivatives for Various Functional Groups and Chemical Entities,” in Design of Prodrugs (H. Bundgaard, ed.), Elsevier, N.Y. (1985); Burger&#39;s Medicinal Chemistry and Drug Chemistry, Fifth Ed., Vol. 1, pp. 172-178, 949-982 (1995). For example, the compounds of Formula I can be transformed into prodrugs by converting one or more of the hydroxy or carboxy groups into esters.  
      The invention also includes isotopically-labeled compounds, which are identical to those recited in Formula I, but for the fact that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine, iodine, and chlorine, such as  3 H,  11 C,  14 C,  18 F,  123 I, and  125 I. Compounds of Formula I that contain the aforementioned isotopes and/or other isotopes of other atoms are within the scope of the invention. Isotopically-labeled compounds of the present invention, for example those into which radioactive isotopes such as  3 H and  14 C are incorporated, are useful in drug and/or substrate tissue distribution assays. Tritiated, i.e.,  3 H, and carbon-14, i.e.,  14 C, isotopes are particularly useful in PET (positron emission tomography), and  125 I isotopes are particularly useful in SPECT (single photon emission computed tomography); all useful in brain imaging. Further, substitution with heavier isotopes such as deuterium, i.e.,  2 H, can afford certain therapeutic advantages resulting from greater metabolic stability, for example increased in vivo half-life or reduced dosage requirements and, hence, may be preferred in some circumstances. Isotopically labeled compounds of Formula I of this invention can generally be prepared by carrying out the synthetic procedures by substituting a isotopically labeled reagent for a non-isotopically labeled reagent.  
      The compounds of Formula I are antagonists at the CRF 1  receptor, capable of inhibiting the specific binding of CRF to CRF 1  receptor and antagonizing activities associated with CRF 1  receptor. The effectiveness of a compound as a CRF receptor antagonist may be determined by various assay methods. A compound of Formula I may be assessed for activity as a CRF antagonist by one or more generally accepted assays for this purpose, including (but not limited to) the assays disclosed by DeSouza et al. ( J. Neuroscience  7: 88, 1987) and Battaglia et al. ( Synapse  1: 572, 1987). CRF receptor affinity may be determined by binding studies that measure the ability of a compound to inhibit the binding of a radiolabeled CRF (e.g., [ 125 I]tyrosine-CFR) to its receptor (e.g., receptors prepared from rat cerebral cortex membranes). The radioligand binding assay described by DeSouza et al. (supra, 1987) provides an assay for determining a compound&#39;s affinity for the CRF receptor. Such activity is typically calculated from the IC 50  as the concentration of a compound necessary to displace 50% of the radiolabeled ligand from the receptor, and is reported as a “Ki” value. IC 50  and Ki values are calculated using standard methods known in the art, such as with the non-linear curve-fitting program GraphPad Prism (GraphPad Software, San Diego, Calif.). A compound is considered to be active if it has an Ki of less than about 10 micromolar (μM) for the inhibition of CRF 1  receptors. The binding affinity of the compounds of Formula I expressed as Ki values generally ranges from about 0.5 nanomolar to about 10 micromolar. Preferred compounds of Formula I exhibit Ki value of 1 micromolar or less, more preferred compounds of Formula I exhibit Ki values of less than 100 nanomolar, still more preferred compounds of Formula I exhibit Ki values of less than 10 nanomolar.  
      In addition to inhibiting CRF receptor binding, a compound&#39;s CRF receptor antagonist activity may be established by the ability of the compound to antagonize an activity associated with CRF. For example, CRF is known to stimulate various biochemical processes, including adenylate cyclase activity. Therefore, compounds may be evaluated as CRF antagonists by their ability to antagonize CRF-stimulated adenylate cyclase activity by, for example, measuring cAMP levels. The CRF-stimulated adenylate cyclase activity assay described by Battaglia et al. (supra, 1987) provides an assay for determining a compound&#39;s ability to antagonize CRF activity. Alternatively, adenylate cyclase activity or cAMP production can be assessed in a 96/384-well format utilizing the cAMP competitive ELISA system from Applied Biosystems (Bedford, Mass.) according to the protocols provided. Briefly, a fixed amount of diluted cAMP-alkaline phosphatase conjugate (cAMP-AP) is added to 96 or 386-well plates containing samples from cells that were stimulated with CRF in the presence or absence of inhibitors. Anti-cAMP antibody is added to the mixture and incubated for 1 hr. Following successive wash steps, the chemiluminescent substrate/enhancer solution is added which then produces a light signal that can be detected using a microplate scintillation counter such as the Packard TopCount. cAMP produced by the cells will displace the cAMP-AP conjugate from the antibody yielding a decrease of detectable signal. An example of the CRF-stimulated adenylate cyclase activity assay is provided in Example C below.  
      Thus, in another aspect, the present invention provides a method of antagonizing CRF 1  receptors in a warm-blooded animal, comprising administering to the animal a compound of the invention at amount effective to antagonize CRF 1  receptors. The warm-blooded animal is preferably a mammal, and more preferably a human.  
      In another aspect, the present invention provides a method of treating a disorder in a warm-blooded animal, which disorder manifests hypersecretion of CRF, or the treatment of which disorder can be effected or facilitated by antagonizing CRF 1  receptors, comprising administering to the animal a therapeutically effective amount of a compound of the invention. The warm-blooded animal is preferably a mammal, and more preferably a human.  
      In another aspect, the present invention provides a method for screening for ligands for CRF 1  receptors, which method comprises: a) carrying out a competitive binding assay with CRF 1  receptors, a compound of Formula I which is labeled with a detectable label, and a candidate ligand; and b) determining the ability of said candidate ligand to displace said labeled compound. Assay procedure for competitive binding assay is well known in the art, and is exemplified in Example A.  
      In another aspect, the present invention provides a method for detecting CRF, receptors in tissue comprising: a) contacting a compound of Formula i, which is labeled with a detectable label, with a tissue, under conditions that permit binding of the compound to the tissue; and b) detecting the labeled compound bound to the tissue. Assay procedure for detecting receptors in tissues is well known in the art.  
      In another aspect, the present invention provides a method of inhibiting the binding of CRF to CRF 1  receptors, comprising contacting a compound of the invention with a solution comprising cells expressing the CRF 1  receptor, wherein the compound is present in the solution at a concentration sufficient to inhibit the binding of CRF to the CRF 1  receptor. An example of the cell line that expresses the CRF 1  receptor and can be used in the in vitro assay is IMR32 cells known in the art.  
      Compounds of Formula I, or a stereoisomer, a pharmaceutically acceptable salt, or a prodrug thereof, are useful for the treatment of a disorder in a warm-blooded animal, which disorder manifests hypersecretion of CRF, or the treatment of which disorder can be effected or facilitated by antagonizing CRF 1  receptors. Examples of such disorders are described herein above. They are also useful for promoting smoking cessation or promoting hair growth.  
      Thus, in still another aspect, the present invention provides a method of treating a disorder described herein above, comprising administering to a warm-blooded animal a therapeutically effective amount of a compound of the invention. The warm-blooded animal is preferably a mammal, particularly a human.  
      Particular disorders that can be treated by the method of the invention preferably include the following: anxiety-relatred disorders (such as generalized anxiety disorder; social anxiety disorder; anxiety; anxiety with co-morbid depressive illness, obsessive-compulsive disorder, and panic disorder); mood disorders (such as depression, including major depression, single episode depression, recurrent depression, child abuse induced depression, and postpartum depression); bipolar disorders; post-traumatic stress disorder; substance abuse disorder (e.g., nicotine, cocaine, ethanol, opiates, or other drugs); inflammatory disorders (such as rheumatoid arthritis and osteoarthritis); gastrointestinal diseases (such as irritable bowel syndrome, ulcers, Crohn&#39;s disease, spastic colon, diarrhea, and post operative ilius and colonic hypersensitivity associated by psychopathological disturbances or stress); inflammatory disorder; and skin disorders (such as acne, psoriasis, and chronic contact demertitis).  
      Particular disorders that can be treated by the method of the invention more preferably include the following: anxiety-related disorders; mood disorders; inflammation disorders; and chronic contact demertitis.  
      Particular disorders that can be treated by the method of the invention even more preferably include anxiety-related disorders, particularly generalized anxiety, and mood disorders, particularly major depression.  
      The therapeutically effective amounts of the compounds of the invention for treating the diseases or disorders described above in a warm-blooded animal can be determined in a variety of ways known to those of ordinary skill in the art, e.g., by administering various amounts of a particular agent to an animal afflicted with a particular condition and then determining the effect on the animal. Typically, therapeutically effective amounts of a compound of this invention can be orally administered daily at a dosage of the active ingredient of 0.002 to 200 mg/kg of body weight. Ordinarily, a dose of 0.01 to 10 mg/kg in divided doses one to four times a day, or in sustained release formulation will be effective in obtaining the desired pharmacological effect. It will be understood, however, that the specific dose levels for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drug combination and the severity of the particular disease. Frequency of dosage may also vary depending on the compound used and the particular disease treated. However, for treatment of most CNS disorders, a dosage regimen of four-times daily or less is preferred. For the treatment of stress and depression, a dosage regimen of one or two-times daily is particularly preferred.  
      A compound of this invention can be administered to treat the above disorders by means that produce contact of the active agent with the agent&#39;s site of action in the body of a mammal, such as by oral, topical, dermal, parenteral, or rectal administration, or by inhalation or spray using appripropriate dosage forms. The term “parenteral” as used herein includes subcutaneous injections, intravenous, intramuscular, intrasternal injection or infusion techniques. The compound can be administered alone, but will generally be administered with a pharmaceutically acceptable carrier, diluent, or excipient.  
      Thus in yet another aspect, the present invention provides a pharmaceutical composition comprising a compound of Formula I, a stereoisomer thereof, a pharmaceutically acceptable salt thereof, or a prodrug thereof, or a pharmaceutically acceptable salt of the prodrug thereof. In one embodiment, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier, diluent, or excipient therefore. A “pharmaceutically acceptable carrier, diluent, or excipient” is a medium generally accepted in the art for the delivery of biologically active agents to mammals, e.g., humans. Such carriers are generally formulated according to a number of factors well within the purview of those of ordinary skill in the art to determine and account for. These include, without limitation: the type and nature of the active agent being formulated; the subject to which the agent-containing composition is to be administered; the intended route of administration of the composition; and the therapeutic indication being targeted. Pharmaceutically acceptable carriers and excipients include both aqueous and non-aqueous liquid media, as well as a variety of solid and semi-solid dosage forms. Such carriers can include a number of different ingredients and additives in addition to the active agent, such additional ingredients being included in the formulation for a variety of reasons, e.g., stabilization of the active agent, well known to those of ordinary skill in the art. Descriptions of suitable pharmaceutically acceptable carriers, and factors involved in their selection, are found in a variety of readily available sources, e.g., Remington&#39;s Pharmaceutical Sciences, 17 th  ed., Mack Publishing Company, Easton, Pa., 1985, the contents of which are incorporated herein by reference.  
      Compositions intended for oral use may be in the form of tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion, hard or soft capsules, or syrups, or elixirs, and can be prepared according to methods known to the art. Such compositions may contain one or more agents selected from the group consisting of sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide pharmaceutically elegant and palatable preparations.  
      Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients, which are suitable for the manufacture of tablets. These excipients may be for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid; binding agents, for example starch, gelatin or acacia, and lubricating agents, for example magnesium stearate, stearic acid or talc. The tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and a delay material such as glyceryl monosterate or glyceryl distearate may be employed.  
      Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin or olive oil.  
      Aqueous suspensions contain the active materials in admixture with excipients suitable for the manufacture of aqueous suspensions. Such excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydropropylmethylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally-occurring phosphatide, for example, lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexital such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate. The aqueous suspensions may also contain one or more preservatives, for example ethyl, or n-propyl p-hydroxybenzoate, one or more coloring agents, one or more sweetening agents, such as sucrose or saccharin.  
      Oily suspensions may be formulated by suspending the active ingredients in a vegetable oil, for example arachis oil, olive oil, soybean oil, sesame oil or coconut oil, or in a mineral oil such as liquid paraffin. The oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide palatable oral preparations. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.  
      Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients, for example sweetening, flavoring and coloring agents, may also be present.  
      Pharmaceutical compositions of the invention may also be in the form of oil-in-water emulsions. The oily phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example liquid paraffin or mixtures of these. Suitable emulsifying agents may be naturally-occuring gums, for example gum acacia or gum tragacanth, naturally-occuring phosphatides, for example soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol, anhydrides, for example sorbitan monooleate, and condensation products of the said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monooleate. The emulsions may also contain sweetening and flavoring agents.  
      Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents.  
      Suppositories for rectal administration of a compound of the invention can be prepared by mixing the compound with a suitable non-irritating excipient, which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug. Examples of such materials are cocoa butter and polyethylene glycols.  
      Pharmaceutical compositions may be in the form of a sterile injectable aqueous or oleaginous suspension. This suspension may be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents, which have been mentioned above. The sterile injectable solution or suspension may be formulated in a non-toxic parentally acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringers&#39;s solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.  
      Dosage forms suitable for administration generally contain from about 1 mg to about 100 mg of active ingredient per unit. In these pharmaceutical compositions, the active ingredient will ordinarily be present in an amount of about 0.5 to 95% by weight based on the total weight of the composition. Examples of dosage forms for administration of compounds of the invention includes the following: (1) Capsules. A large number of units capsules are prepared by filling standard two-piece hard gelatin capsules each with 100 mg of powdered active ingredient, 150 mg lactose, 50 mg cellulose, and 6 mg magnesium stearate; (2) Soft Gelatin Capsules. A mixture of active ingredient in a digestible oil such as soybean, cottonseed oil, or olive oil is prepared and injected by means of a positive displacement into gelatin to form soft gelatin capsules containing 100 mg of the active ingredient. The capsules were washed and dried; (3) Tablets. A large number of tablets are prepared by conventional procedures so that the dosage unit was 100 mg active ingredient, 0.2 mg of colloidal silicon dioxide, 5 mg of magnesium stearate, 275 mg of microcrystalline cellulose, 11 mg of starch, and 98.8 mg lactose. Appropriate coatings may be applied to increase palatability or delayed adsorption.  
      In still another aspect, the present invention provides an article of manufacture comprising: a) a packaging material; b) a pharmaceutical agent comprising a compound of the invention contained within said packaging material; and c) a label or package insert which indicates that said pharmaceutical agent can be used for treating a disorder described above.  
     Definitions and Conventions  
      The following definitions are used throughout the application, unless otherwise described.  
      The term “halogen” means a group selected from —F, —Cl, —Br, or —I.  
      The term “alkyl” means both straight- and branched-chain hydrocarbon moieties having from 1-10 carbon atoms optionally containing one or more double or triple bonds;  
      The term “substituted alkyl” means an alkyl moiety having 1-5 substitutents independently selected from halogen, oxo (═O), thione (═S), —NO 2,  —CN, —R a , —OR a , —S(O) m R a , —NR a R a , —C(O)NR a R a , —C(S)NR a R a , —S(O) m NR a R a , —NR a S(O) m R a , —NR a C(O)OR a , —OC(O)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)OR a , OC(O)R a , OC(S)R a , NR a C(S)OR a , and OC(S)NR a R a ;  
      The term “haloalkyl” means an alkyl moiety having 1 to (2v+1) independently selected halogen substituent(s) where v is the number of carbon atoms in the moiety;  
      The term “cycloalkyl” means a a monocyclic, non-aromatic hydrocarbon moiety, having from 3-10 carbon atoms or a bicyclic non-aromatic hydrocarbon moiety, having from 4 to 10 carbon atoms, optionally containing 1 to 2 double bonds;  
      The term “substituted cycloalkyl” means a cycloalkyl moiety having 1-5 substitutents independently selected from halogen, oxo (═O), thione (═S), —NO 2,  —CN, —R a , —OR a , —S(O) m R a , —NR a R a , —C(O)NR a R a , —C(S)NR a R a , —S(O) m NR a R a , —NR a S(O) m R a , —NR a C(O)OR a , —OC(O)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)OR a , OC(O)R a , OC(S)R a , NR a C(S)OR a , and OC(S)NR a R a ;  
      The term “aryl” means either phenyl or naphthyl;  
      The term “substituted aryl” means an aryl group substituted with 1-5 substituents independently selected from halogen, —NO 2,  —CN, —R a , —OR a , —S(O) m R a , —NR a R a , —C(O)NR a R a , —C(S)NR a R a , —S(O) m NR a R a , —NR a S(O) m R a , —NR a C(O)OR a , —NR a C(S)OR a , —OC(O)NR a R a , —OC(S)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)R a , OC(S)R a , —OC(O)OR a , OC(O)R a , OC(S)R a , NR a C(S)OR a , and OC(S)NR a R a ;  
      The term “heteroaryl” means a radical of a monocyclic aromatic ring containing five or six ring atoms consisting of carbon and 1 to 4 heteroatoms each selected from the group consisting of non-peroxide O, S, N, with appropriate bonding to satisfy valence requirements, wherein the attachment may be via a ring carbon or ring N where a N is present. The term “heteroaryl” also includes a radical of a fused bicyclic heteroaromatic ring having eight to ten ring atoms consisting of carbon and 1 to 6 heteroatoms each selected from non-peroxide O, S, N, with appropriate bonding to satisfy valence requirements, wherein the attachment may be via a ring carbon or ring N where a N is present. Examples of heteroaryl include thienyl, benzothienyl, pyridyl, thiazolyl, quinolyl, pyrazinyl, pyrimidyl, imidazolyl, furanyl, benzofuranyl, benzothiazolyl, isothiazolyl, benzisothiazolyl, benzisoxazolyl, benzimidazolyl, indolyl, and benzoxazolyl, pyrazolyl, triazolyl, tetrazolyl, isoxazolyl, oxazolyl, pyrrolyl, isoquinolinyl, cinnolinyl, indazolyl, indolizinyl, phthalazinyl, pydridazinyl, triazinyl, isoindolyl, purinyl, oxadiazolyl, furazanyl, benzofurazanyl, benzothiophenyl, benzothiazolyl, quinazolinyl, quinoxalinyl, naphthridinyl, and furopyridinyl;  
      The term “substituted heteroaryl” means a heteroaryl group having 1-5 substituents independently selected from halogen, —NO 2,  —CN, —R a , —OR a z, S(O) m R a , —NR a R a , —C(O)NR a R a , —C(S)NR a R a , —S(O) m NR a R a , —NR a S(O) m R a , —NR a C(O)OR a , —OC(O)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)OR a , OC(O)R a , OC(S)R a , NR a C(S)OR a , and OC(S)NR a R a ;  
      The term “heterocycloalkyl”, unless otherwise specified, means a 3 to 8 membered monocyclic non-aromatic ring or a 4 to 8 membered bicyclic non-aromatic ring, wherein at least one carbon atom is replaced with a heteroatom selected from oxygen, nitrogen, —NH—, or —S(O) m — wherein m is zero, 1, or 2, optionally containing from one to three double bonds, and wherein the ring attachment can occur at either a carbon or nitrogen atom. Examples of heterocycloalkyl includes tetrahydrofuranyl, tetrahydropyranyl, morpholinyl, pyrrolidinyl, piperidinyl, piperazinyl, [2.2.1]-azabicyclic rings, [2.2.2]-azabicyclic rings, [3.3.1]-azabicyclic rings, quinuclidinyl, azetidinyl, azetidinonyl, oxindolyl, dihydroimidazolyl, and pyrrolidinonyl;  
      The term “substituted heterocycloalkyl” is a heterocycloalkyl group having 1-5 substituents independently selected from halogen, oxo (═O), thione (═S), —NO 2 , —CN, —R a , OR a , —S(O) m R a , —NR a R a , —C(O)NR a R a , —C(S)NR a R a , —S(O) m NR a R a , —NR a S(O) n R a , —NR a C(O)OR a , —OC(O)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)OR a , OC(O)R a , OC(S)R a , NR a C(S)OR a , and OC(S)NR a R a ;  
      The term “aryl cycloalkyl” means a bicyclic ring system containing 8 to 14 carbon atoms wherein one ring is aryl and the other ring is fused to the aryl ring and may be fully or partially saturated in the portion of the ring fused to the aryl ring, wherein either ring may act as a point of attachment;  
      The term “substituted aryl cycloalkyl” means an aryl cycloalkyl group having 1-5 substituents independently selected from halogen, oxo (═O), thione (═S), —NO 2,  —CN, —R a , —OR a , —S(O) m R a , —NR a R a , —C(O)NR a R a , —C(S)NR a R a , S(O) m NR a R a , —NR a S(O) m R a , —NR a C(O)OR a , —OC(O)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)OR a , OC(O)R a , OC(S)R a , NR a C(S)OR a , and OC(S)NR a R a ;  
      The term “heteroaryl cycloalkyl” means a bicyclic ring system containing 8 to 14 atoms, wherein one ring is heteroaryl and the other ring is fused to the heteroaryl ring and may be fully or partially saturated in the portion of the ring fused to the heteroaryl ring, wherein either ring may act as a point of attachment;  
      The term “substituted heteroaryl cycloalkyl” means a heteroaryl cycloalkyl group having 1-5 substituents independently selected from halogen, oxo (═O), thione (═S), —NO 2,  —CN, —R a , —OR a , —S(O) m R a , —NR a R a , —C(O)NR a R a , —C(S)NR a R a , —S(O) m NR a R a , —NR a S(O) m R a , —NR a C(O)OR a , —OC(O)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)OR a , OC(O)R a , OC(S)R a , NR a C(S)OR a , and OC(S)NR a R a ;  
      The term “aryl heterocycloalkyl” means a bicyclic ring system containing 8 to 14 atoms, wherein one ring is aryl and the other ring is heterocycloalkyl, wherein either ring may act as a point of attachment;  
      The term “substituted aryl heterocycloalkyl” means an aryl heterocycloalkyl group having 1-5 substituents independently selected from halogen, oxo (═O), thione (═S), —NO 2,  —CN, —R a , —OR a , S(O) m R a , —NR a R a , —C(O)NR a R a , —C(S)NR a R a , —S(O) m NR a R a , —NR a S(O) m R a , —NR a C(O)OR a , —OC(O)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)OR a , OC(O)R a , OC(S)R a , NR a C(S)OR a , and OC(S)NR a R a ;  
      The term “heteroaryl heterocycloalkyl” means a bicyclic ring system containing 8 to 14 atoms, wherein one ring is heteroaryl and the other ring is heterocycloalkyl, wherein either ring may act as a point of attachment;  
      The term “substituted heteroaryl heterocycloalkyl” means an heteroaryl heterocycloalkyl group having 1-5 substituents independently selected from halogen, oxo (═O), thione (═S), —NO 2,  —CN, —R a , —OR a , —S(O) m R a , —NR a R a , —C(O)NR a R a , —C(S)NR a R a , —S(O) m NR a R a , —NR a S(O) m R a , —NR a C(O)OR a , —OC(O)NR a R a , —NR a C(O)NR a R a , —NR a C(S)NR a R a , —C(O)OR a , —C(S)OR a , —OC(O)OR a , OC(O)R a , OC(S)R a , NR a C(S)OR a , and OC(S)NR a R a ;  
      The term “pharmaceutically acceptable,” unless otherwise described, refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problems or complications, commensurate with a reasonable benefit/risk ratio.  
      The term “pharmaceutically acceptable salt” refers to a salt which retains the biological effectiveness and properties of the compounds of this invention and which is not biologically or otherwise undesirable.  
      The term “stereoisomer” refers to a compound made up of the same atoms bonded by the same bonds but having different three-dimensional structures which are not interchangeable. The three-dimensional structures are called configurations. As used herein, the term “enantiomer” refers to two stereoisomers whose molecules are nonsuperimposable mirror images of one another. The term “chiral center” refers to a carbon atom to which four different groups are attached. As used herein, the term “diastereomers” refers to stereoisomers which are not enantiomers. In addition, two diastereomers which have a different configuration at only one chiral center are referred to herein as “epimers”. The terms “racemate” or “racemic mixture” refer to a mixture of equal parts of enantiomers.  
      The term “prodrug” means compounds that are transformed in vivo to yield a compound of Formula I. The transformation may occur by various mechanisms, such as through hydrolysis in blood.  
      The term “therapeutically effective amount,” “effective amount,” “therapeutic amount,” or “effective dose” is meant that amount sufficient to elicit the desired pharmacological or therapeutic effects, thus resulting in effective prevention or treatment of the disease.  
      The phrases “a compound of the invention,” “a compound of the present invention,” “compounds of the present invention,” or “a compound in accordance with Formula I” and the like, refer to compounds of Formula I, or stereoisomers thereof, pharmaceutically acceptable salts thereof, or prodrugs thereof, or pharmaceutically acceptable salts of a prodrug of compounds of Formula I.  
      The terms “treatment,” “treat,” “treating,” and the like, are meant to include both slowing or reversing the progression of a disorder, as well as curing the disorder. These terms also include alleviating, ameliorating, attenuating, eliminating, or reducing one or more symptoms of a disorder or condition, even if the disorder or condition is not actually eliminated and even if progression of the disorder or condition is not itself slowed or reversed. The term “treatment” and like terms also include preventive (e.g., prophylactic) and palliative treatment. Prevention of the disease is manifested by a prolonging or delaying of the onset of the symptoms of the disease.  
     EXAMPLES  
      Without further elaboration, it is believed that one skilled in the art can, using the preceding description, practice the present invention to its fullest extent. The following examples are provided to illustrate the invention and are not to be construed as limiting the invention in scope or spirit to the specific procedures described in them. Examples A-D are provided to illustrate biological assays that can be used for determining the biological properties of the compounds of the inventions. Those skilled in the art will promptly recognize appropriate variations from the procedures described in the examples.  
     Example A  
      In Vitro CRF 1  Receptor Binding Assay for the Evaluation of Biolocical Activity  
      The following is a description of a standard in vitro binding assay for the evaluation of biological activity of a test compound on CRF 1  receptors. It is based on a modified protocol described by De Souza (De Souza, 1987).  
      The binding assay utilizes brain membranes, commonly from rats. To prepare brain membranes for binding assays, rat frontal cortex is homogenized in 10 mL of ice cold tissue buffer (50 mM HEPES buffer pH 7.0, containing 10 mM MgCl 2 , 2 mM EGTA, 1 μg/mL aprotinin, 1 μg/mL leupeptin and 1 μg/mL pepstatin). The homogenate is centrifuged at 48,000×g for 10 min. and the resulting pellet rehomogenized in 10 mL of tissue buffer. Following an additional centrifugation at 48,000×g for 10 min., the pellet is resuspended to a protein concentration of 300 μg/mL.  
      Binding assays are performed in 96 well plates at a final volume of 300 μL. The assays are initiated by the addition of 150 μL membrane suspension to 150 μL of assay buffer containing  125 I-ovine-CRF (final concentration 150 pM) and various concentrations of inhibitors. The assay buffer is the same as described above for membrane preparation with the addition of 0.1% ovalbumin and 0.15 mM bacitracin. Radioligand binding is terminated after 2 hours at room temperature by filtration through Packard GF/C unifilter plates (presoaked with 0.3% polyethyleneimine) using a Packard cell harvestor. Filters are washed three times with ice cold phosphate buffered saline pH 7.0 containing 0.01% Triton X-100. Filters are assessed for radioactivity in a Packard TopCount.  
      Alternatively, tissues and cells that naturally express CRF receptors, such as IMR-32 human neuroblastoma cells (ATCC; Hogg et al., 1996), can be employed in binding assays analogous to those described above.  
      A compound is considered to be active if it has a Ki value of less than about 10 μM for the inhibition of CRF. Nonspecific binding is determined in the presence of excess (10 μM) α-helical CRF.  
     Example B  
      Ex Vivo CRF 1  Receptor Binding Assay for the Evaluation of Biological Activity  
      The following is a description of a typical ex vivo CRF 1  receptor binding assay for assessing the biological activity of a test compound on CRF 1  receptors.  
      Fasted, male, Harlen-bred, Sprague-Dawley rats (170-210 g) were orally dosed with test compound or vehicle, via gastric lavage between 12:30 and 2:00 PM. Compounds were prepared in vehicle (usually 10% soybean oil, 5% polysorbate 80, in dH2O). Two hours after drug administration, rats were sacrificed by decapitation, frontal cortices were quickly dissected and placed on dry ice, then frozen at −80° C. until assayed; trunk blood was collected in heparinized tubes, plasma separated by centrifugation (2500 RPM&#39;s for 20 minutes), and frozen at −20° C.  
      On the day of the binding assay, tissue samples were weighed and allowed to thaw in ice cold 50 mM Hepes buffer (containing 10 mM MgCl 2 , 2 mM EGTA, 1 μg/mL aprotinin, 1 μg/mL leupeptin hemisulfate, and 1 μg/mL pepstatin A, 0.15 mM bacitracin, and 0.1% ovalalbumin, pH=7.0 at 23° C.) and then homogenized for 30 sec at setting 5 (Polytron by Kinematica). Homogenates were incubated (two hours, 23° C., in the dark) with [ 125 I] CRF (0.15 nM, NEN) in the presence of assay buffer (as described above) or DMP-904 (10 uM). The assay was terminated by filtration (Packard FilterMate, GF/C filter plates); plates were counted in Packard TopCount LSC; total and non-specific fmoles calculated from DPM&#39;s. Data are expressed as % of vehicle controls (specific fmoles bound). Statistical significance was determined using student&#39;s t-test.  
     Example C  
      Inhibition of CRF Stimulated Adenylate Cyclase Activity  
      Inhibition of CRF-stimulated adenylate cyclase activity can be performed as previously described [G. Battaglia et al., Synapse 1: 572 (1987)]. Briefly, assays are carried out at 37° C. for 10 min in 200 mL of buffer containing 100 mM Tris-HCl (pH 7.4 at 37° C.), 10 mM MgCl 2 , 0.4 mM EGTA, 0.1% BSA, 1 mM isobutylmethylxanthine (IBMX), 250 units/mL phosphocreatine kinase, 5 mM creatine phosphate, 100 mM guanosine 5′-triphosphate, 100 nM o-CRF, antagonist peptides (various concentrations) and 0.8 mg original wet weight tissue (approximately 40-60 mg protein). Reactions are initiated by the addition of 1 mM ATP/[ 32 P]ATP (approximately 2-4 mCi/tube) and terminated by the addition of 100 mL of 50 mM Tris-HCl, 45 mM ATP and 2% sodium dodecyl sulfate. In order to monitor the recovery of cAMP, 1 mL of [ 3 H]cAMP (approximately 40,000 dpm) is added to each tube prior to separation. The separation of [ 32 P]cAMP from [ 32 P]ATP is performed by sequential elution over Dowex and alumina columns.  
      Alternatively, adenylate cyclase activity can be assessed in a 96-well format utilizing the Adenylyl Cyclase Activation FlashPlate Assay from NEN Life Sciences according to the protocols provided. Briefly, a fixed amount of radiolabeled cAMP is added to 96-well plates that are precoated with anti-cyclic AMP antibody. Cells or tissues are added and stimulated in the presence or absence of inhibitors. Unlabeled cAMP produced by the cells will displace the radiolabeled cAMP from the antibody. The bound radiolabeled cAMP produces a light signal that can be detected using a microplate scintillation counter such as the Packard TopCount. Increasing amounts of unlabeled cAMP results in a decrease of detectable signal over a set incubation time (2-24 hours).  
     Example D  
      In Vivo Biological Assay  
      The in vivo activity of a compound of the present invention can be assessed using any one of the biological assays available and accepted within the art. Illustrative of these tests include the Acoustic Startle Assay, the Stair Climbing Test, and the Chronic Administration Assay. These and other models useful for the testing of compounds of the present invention have been outlined in C. W. Berridge and A. J. Dunn Brain Research Reviews 15: 71 (1990). A compound may be tested in any species of rodent or small mammal.